LOCUS       DAOPKE010000001        91455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_210_length_91455_cov_11.878315, whole genome shotgun sequence.
ACCESSION   DAOPKE010000001 DAOPKE010000000
VERSION     DAOPKE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 91455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 91455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_91455_cov_11.878315"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3865)
                     /locus_tag="PKY63_00005"
     CDS             complement(<1..3865)
                     /locus_tag="PKY63_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPE99020.1"
                     /translation="MSDKNILSPRKALNKAFLKMKPLRSDITLFKNNLVQLLDDCNDS
                     ESEEFHKNLLSTFLYKTYYSDKHFINTKGRNDLVIHNGKDAKSPVGVIVETKKPGNKT
                     EMLRPGNVNTKAMQELVLYYLRERITLNNLQLKYLIATDIFEWYIFDAQLFEKLFAQN
                     KKLVQLFNDFEAGRLGGNKTEFFYKEVAQPAIEAVKENIVFTHFDISEYEKPLRNSEI
                     SDDNKLIALQKIFSPEHLLKKQFSNDSNSLNKAFYNELLHIIGLTEIKDGSKKIITRK
                     KEQERNAGSLLENAISQIDNLDKFSVIPNPSRFGETSEERYFNLGLELCITWINRILF
                     LKLLEAQLLSYHKGNSEFRFMSIHKISSFDELNQLFFSVLAKLPDERNARVKARFAKV
                     PYLNSSLFEQSEIEQQSIFISNLSDEEMLPLIANSVLRRSDKYSKAKDVSSLEYLFEF
                     LDAYDFSSDGSGEIQEDNKNLINASVLGLIFEKINGYRDGSFFTPGFITMYMCHETIR
                     RAVVQKFNETKGWSCNTLDELYNKIEDSAEANRIVNSIKICDPAVGSGHFLVSALNEL
                     ISIKSELRILCDREGRRLKEYNVEIQNDELILTDEDGEIFEYKPGNKESQRVQEALFH
                     EKQTLIENCLFGVDINPNSVKICRLRLWIELLKNAYYKSSGNPKNVTLSGVEGSFAGL
                     TELETLPNIDINIKCGNSLISRYALDADIKQALKKSQWTIDAYKVAIQSYREASSKET
                     KREMEKLINQIKSDFESEIAINDKRLKQLNLLKGELVSLTTEVTMFDRSAKEKAAWNK
                     KVEKLTGEITSIEKDLEEIKNNKIYDNAFEWRFEFPEVLNDNGDFIGFDVVIGNPPYI
                     RQEDFSVIKPYLQSHFETFAGTADLYVYFVERGLQITKQNGSFIYILPNKWMRAGYGN
                     ALRQWVQQYTIGFIHDFGDLPVFEEATTYPCLWNIIKQKPTQNTFISSNIQTLDFTDR
                     LESYIQTIAFKVNSEMLSDSGWTLVDDSKQKLLEKIKSKGIPLGEYVNGKIFRGVLTG
                     LNEAFVIDEETKSRMIAENPSSAEIIKPFLAGRDIKRYQQPKSDKYLILIPKGFTIKS
                     FLQIDSFKANEPPPRYGNMEYDAAWEWFSKKYPAVANHLLPFKSKATVRTDKGDFWWE
                     LRACDYYEEFEKGKIIYPNICKQPEFVYDTSKQYTNQKCFIIPEADKYLLALLNSRVT
                     YFLFQNLLPKLRGDFYEPSYLYFKDFPIVESENRSEFITIADQIIDAKSFNAAADTTA
                     LESEIDRLVYELYGLSEEEIGIVEGE"
     gene            complement(4109..4528)
                     /locus_tag="PKY63_00010"
     CDS             complement(4109..4528)
                     /locus_tag="PKY63_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99021.1"
                     /translation="MKPLSLLFLSLLLLSYCGFSQQASLTIINKSDRVMTTKVIKGTE
                     RTAELYKTLTIAPKGKETIYFNTTGRYFTKTMAVLISKDTLTLNDTLYSKGTPFEVIA
                     DSKRGYSNITMKFIVKESKKPILDGSFPITRKEYDDD"
     gene            complement(4600..5568)
                     /locus_tag="PKY63_00015"
     CDS             complement(4600..5568)
                     /locus_tag="PKY63_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99022.1"
                     /translation="MKTLLYTFIILCIVTTASAQHHFPTPAEDPFWMLEHGQLFQHEC
                     TPGCGYYTSCMLPVYYKSDTVINATNYHRLYKRSVCHSILTSPSPYCIFSCDFNIPEE
                     LYAIIRIDSATNSVWQYSEWDNNEHLLFDFNLSVGQVYPPTINNPYISSTDTLMVLSE
                     DSVLLGNTYHKKWNLGVKISGSIHDSAFVSIIEGVGSTFGLLGELYVPFESSDQMYCF
                     SMADTVLFPNSSQVCDQTIGLSEIQTIPSFEIYPNPAQNIIQLNLIYIENEINVTLIN
                     TRGQKISGQIISGNAATIDVSMLTPGLYFLRIGSGRNTGMKGFLKE"
     gene            complement(5638..8289)
                     /locus_tag="PKY63_00020"
     CDS             complement(5638..8289)
                     /locus_tag="PKY63_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE99023.1"
                     /translation="MTSKNITESKLAIDKLKQEHDHMVDVLESMSDAFVSIDFNWCYT
                     YMNKKAGEIFERNPADIIGKHIWTEFPEGIGQPFQLNYEKVMTERIPISLEEYYPPYD
                     RWFENHIYPTENGISVFFHDITNTKKNELQLKESELRYRAIFENTGTAAVIIEEDTTI
                     SLANSKFEELSGYSKDEIENRKTWKEFVVWEDLVRMEEQHMLRRIRQESALKSYEFRF
                     VGKNGVQKYILLTVDIIPGTKRSVASLLDITDIKTTEHKLRESEASYRLLFEQNPLPM
                     LIYERGSLEVISVNESFLKHYGYSVKDISNMHLQDFYPPELRNGIVDLARKIQGHSYT
                     GEWKHLKADGSLIDILVTSNDIKYNNRNARIAVINDITERKRIEQTVLQSEKQLKEAQ
                     HVAKLGNWEYCFTNSEIKWSDEMYIIFEINKNTEKDLFKSFIERIHPEDRIKAKKEFD
                     KSANNKDGIQFEHRLLMPDGRVKYVFVNCIPHVSKGEKMQKSAGTMQDITGRKIIEIE
                     LQRNRTMLRSMIDSLPMWLAAVDLDGKYFIANQYYSKTFKLPLNNIENHNFNEFFKPD
                     LYKRHKAFLDKCRKTQKAVEVSDQVEFENGKITHIYGAYTPLFDTSGELFGFSAAIMD
                     ISKQKAIEAEIIQLNSTLEEKVKQRTNELVKTNESLTVEINERLKAEEQIKHQLQEKE
                     VLLQEIHHRVKNNMQVIVSIINLQLSTVTDEGIRRILRDSQARIKTMALVHEKLYETT
                     DFSNIDLIDYIKNLFEFLTSIYKSPDENIQCKISGKSYKVNIDAIIAIGLITNEIITN
                     SFKYAFEPGSKGLIEVSLTKKDEKNLIYSIKDNGKGLPEDFDCYNTKSLGLQLVCILT
                     EQINGSLNVNSSSKGTEFVITFPAKVQ"
     gene            complement(8347..8994)
                     /locus_tag="PKY63_00025"
     CDS             complement(8347..8994)
                     /locus_tag="PKY63_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE99024.1"
                     /translation="MSNMFSAESIQRMLDSIYDGIMVLDVDSGKYVYANQCACELYGC
                     SPEEIKELNPADLSVISSAEDEALIIENNKTAKQTGRCVFEWLAKRVDGTTFWAEVAL
                     TYAEINGKPNYIAVVRNITHKKTIEAEKNLTTAQFRAILDSSPDALAIVDYDGCFIDG
                     NKAFLSRWGKKPHELIGHSAMEILPKHIFDSRLEKVRESIQTKKPLNFIDSYNNK"
     gene            9258..12413
                     /locus_tag="PKY63_00030"
     CDS             9258..12413
                     /locus_tag="PKY63_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPE99025.1"
                     /translation="MSRFWKYLFFAVSVLIICNVRAQSAGEVTFDSYSSEIYSYRKGL
                     SQNTVQCIMQDSRGFVWIGTWDGLNRFDGYKFQIFRPDYTNPKGKLSCESINALAEDK
                     NGNLWIGTDKGLNLYRYKADTFQTFYYSFYNSNSPSSDSITSLVIDKSGMLWIGGNKG
                     LCSYDIENKKFTRYYYNSRKDASIPSDLIYALHVDDENRLWIGTNKGMALMDIRSKKI
                     FRLDQLTCKNGQKPLVTSFAEAGNVIWVGTYCGVYVFDKRKLNEYPNNCFKDAGLSAE
                     LTSQPISRLLCDTRGRFWIATPDNGVYVYYPESCELKHIYSDFNSRYGLSNNSMLSLA
                     VSQGGEVWIGTWHGLNMYSDYSYKLPHFRIIGSEFDYSNNLIWSFQQLQSGNLLIGTH
                     GGLIEFDVATRNFSFFSEQEFGKIPVRALTILPDGNIWVGTYMNGLVLMNQQGHVLKR
                     FSEDSPDKIAGNIIWEIVPDNKNNIWVAAYGGLTRINLSTFETRMWKYPGYESKGYIS
                     SSSVHHVMVDKSGIVWISTINGLNRLDPAKDKIYVFHHDQGNPVSISNDRILSTFEDS
                     KGVMWVGTFGGGLCRYDRKLGVFRRFSRAEGFPDNVIYDIIEDRQGFLWLTSNVGVIR
                     FNPRDFSNVKYDVNDGVQSHEFNLGASYISPEGMIAIGGMNGFNYFNTREIKVNRQIP
                     KIVITEFNVFGKSYGSYIEDGDTIVLEPNNNFFSISFSALDYTNPSKNKYAYILKNYD
                     KDWIQTDAARRSAEYTDVKPGTYQFMVKGSNSDGIWNEQGLSFTVIVKPPFYKTWYFR
                     FLLIALALFITWLFIRGRVRKVRQQNEVEVRMLKIEKEMFDLEQKALRLQMNPHFIFN
                     SLNSIQSFIISNDSENAINYLAKFSQLMRLILITSREAYIPLSQEISLLKHYLDLENM
                     RFNNRFQYEIRVESGMDTEYMGIPPMIIQPYIENAIIHGFMNKKTGECRLLVQMWEEE
                     DYILCVIEDNGIGREKAMEIKQKSGLIQKSQGIIITKERLDILNRQLKNKISVEITDL
                     KDDAGNATGTRVRLVIPFIDL"
     gene            12463..13203
                     /locus_tag="PKY63_00035"
     CDS             12463..13203
                     /locus_tag="PKY63_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPE99026.1"
                     /translation="MIRAIIIDDEDASRITLKALLGRYVPDVQIIAEAVDVESGLATV
                     KQFTPDLVFLDIQMPDGSGFRLLEMLPEIDFDVIFTTAFDQYAIKAIKFSALDYLLKP
                     IVSDDLKKAVEKHRQKISAPDRNISYRTLLENLNPEPRKIVLHTFEGMHVVETADIIR
                     CQSDDCYTNFYLNGGKRIIVSKTLKEIEEQLTGKDFIRPHKSHLINLQYIKTVVRNDG
                     GYIVMKDGTEIPISRRKKEMVMDIINKL"
     gene            13309..13827
                     /locus_tag="PKY63_00040"
     CDS             13309..13827
                     /locus_tag="PKY63_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99027.1"
                     /translation="MEQFIGLIIFFVAWIIISAMKKTAKKQQEAAKQMNKNAQMQNAQ
                     AAKPASTADEINKLIEMFTGEYVAPKPASVTTPVEQSYSDSIETIEAINEVGGIETAA
                     PSYRSVDEYVPYSSSIDDFSSEETLQSSFAGQTENKNVFNRSKNLPNPFLTDFDLKKA
                     VVYSVILEPKYF"
     gene            13937..17566
                     /locus_tag="PKY63_00045"
     CDS             13937..17566
                     /locus_tag="PKY63_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPE99028.1"
                     /translation="MFRKLLSALGLTLFVSSMVLAQSSGTLKGKLVDKETKEPIPFAN
                     IIAETGGKQMGGSTTDFDGNYTIKPIPPGTYDVKATYVGYKPLMVQGVVVKDGIITFL
                     DIEMESTAVTLTTFEVVDYKVPLIEKDGGSSGGTVTSEEIEKMAGRDATSVAVTVGGV
                     FSQDGEMGSIRGGREDGTVMYIDGVRVRGSSSLPKSALEQVTVITGGLPANYGDATGG
                     VVNITTKGASRTFGMGAELVTSKFLDAYDYNLLGLFMQGPLLKGRDSTQNTALLGYFL
                     AGEFSYEKDNYPFFDGLYKAKDDYLAQVEQNPLRETGLGYGTYQNLQFVTMDNLEKIK
                     AKIECPSYAANLSLKLDVRTTSTTNLTFGGSLNYSNGLAYVHSYSMFNYKQYPQVISS
                     TWRVFGRFTQRFNADTASKSIVKNVFYNIQADYSKYNYLEQDPDHKDNLFKYGHWGEF
                     NTYKAKSYELGDIDSLGYYNVYLLNNYYDTLVTFEPGSNNPITSNYTSQYYDLYDLHS
                     GYYSNYTSIQNAGALLNGQQPESVYGLWSAPGTIYNSYNKSDATQIGVNANASADIGN
                     HAFQFGIQYEQRKDCAIGYAPVSLWTRMRQLANKHIEQLDLSNPDPQTDMYGVFTDTV
                     EYYRLYDANSQSTFDKNLRESLGLPVDGTDWIDVDSYDPSTYNIEWFSADELLNEGNS
                     YVSYYGYDYTGKKLTSKPSFDDFFTKKDANGNYTREIGAFEPIYMAGYIMDKFAFKDL
                     IFNIGLRVDRFDANQMVLEDKYLFYEAKTADQVTNLGVHPGNIPGSATVYVDNINNPT
                     QILGYRDGDVWYNAQGTQITDPSTISTSSGIAPYLVDPSLTKPTSAAFKDYKPQVTYM
                     PRISFSFPISDEALFFAHYDVTSKRPTSGLRLDPTQYYFIQTNAQSFINNPDLKPEKT
                     VDYELGFQQLLSIKSSLKFSAYYREMRDLVQAYRFYGGYPIDYYSYNNIDFGTVKGMT
                     IAYDLRRSANVWLKASYTLQFADGTGSNSSSGMNLVRTGQPNLRTLNPLDYDRRHAFT
                     LVLDYRFGSGKNYNGPKITKKVGDGKVKVTPLLQNTGVNFTFTGGSGTPYSKSSNIIS
                     SQLGGGSYQLQGMINGSRLPWSFRVDSRIDRDIDVKIGKKDMTLNVYLEILNVLNSKN
                     IMSVYRSTGNPDDDGYLAAAEYQAGIEAQVNEEAFRTLYSILVNSPYNYSLPRRIRFG
                     VAFGF"
     gene            17587..21636
                     /locus_tag="PKY63_00050"
     CDS             17587..21636
                     /locus_tag="PKY63_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99029.1"
                     /translation="MKKNIITSLLFIGLVGLAMGSYARKVPIAENRSQQEGKQIRLKS
                     TAEACLPGAVSTELKINNVRARINSGGDMWWDLADVSEYEVPYGSKMTSMFAGALWIG
                     GTDINGQLKLAAHRYRQVGNDFFPGPLTVDGTASVSAETCIKYDKMNVIYRKDVDEFL
                     AWQASPSDYPDYVMPSYFSTYPAMGDELLGQSRYLAPFFDANEDLFYNPLDGDYPYYD
                     IDNDLCHQPDSTLETIEGIVSGGILADQVLKGDQTIWWVFNDKGNVHTETQGAAIGLE
                     IRAQAFAFSTNDEINNMTFYSFEIINRSTYTLQETYMSLWTDPDLGYSHDDYVGCDVL
                     RGLGYAYNGDQYDEDGNGAFGYGDQPPAVGIDFFQGPYMDPDGIDNPAFVKTTDSSGA
                     IVIENCNEAVNGVNFGNGIIDDERYGMRRFVYHNNSGTVPPYMTDPDIAIEYYNFLRG
                     YWKDNTRMLYGGNAHVSSGAYGPECDFMFPDETDPCDWGTGGVPPAGPRKWTEETAGN
                     QPYDRRFMQSAGPFTLKPGALNYITVGLPWARATSGGPFASVELLRRTDDKCQRLFDN
                     CFKVVDGPDAPDLTLQELDKEIILFITNSGSSNNYLEQYEEGDPSIVSPEGEHYDSLY
                     RFEGYQVFQVKDISVSATDLYDTDKARLIAQCDVENDVSRLINFYYSDDLGAVVPREE
                     VNGANEGIVHSFRVLEDQFATGDKRLVNNKKYYFMVIAYAFNEYEKYSQDPAYQIPGI
                     ASLYGQQKPYLAGRKNIETYVGIPHMPSPENSGTLIQSNYGDGPKITRIEGQGNGGMI
                     LEMTEASRNAALINNSILTPTYENGFGPLDIKVIDPLNVKEADYVLRFDTLSTIPDSL
                     HLARWILEEYDLSGNLVASYLSDHSIETKFEQIFVDLGISVTLAQALAPGDQTHPTNG
                     FLQADIIYEDPTRMWLSGVPDIDGPSVYNWIRSGTTDSDDNDQDDFIINGVFGDAGQY
                     YEKLLGGTWAPFRLTSFSTDNPGIAESSVQAWISNTNKLSNLASIDVVFTPDKSKWTR
                     CPVLEMCEDKTLSEGGAVKHSLRKHASVDKNGNTGTPEATYDGYAMGMGWFPGYVINI
                     ETGERLNVAFGEDSWLIGENGRDMLFNPTANYEALTQTLFGGKHFLYIFGHKGDNVND
                     CPAYDGGKWLADKYNLNTTVAWRHIYSCVMYTSIPLSVPNEDWLSNEARVRIRVSKPY
                     AINYSTYGSATAQNKNYPMYSFNTSDIATKTNDLETAKTALDLINVVPNPYYANSGYE
                     ETQLDNKVKITNLPSKCTVSIYTVDGMLIRRYTRDDPNSTYIDWDLKNSANIPISGGL
                     YLIHVDAPGVGERTIKWFGSLRPIDLNSF"
     gene            21662..22720
                     /locus_tag="PKY63_00055"
     CDS             21662..22720
                     /locus_tag="PKY63_00055"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPE99030.1"
                     /translation="MKNIVKNLFAIMLLVALTIPVNPVFAGNEDRAGESGAGELLIMP
                     FARSSGWGMSNTSCIRGLEGMYLNVAGTAFTKSTEVMFARTEWLKGSGISINSFGLTQ
                     KVGTSGVIGLGVMTMGFGDIMITTVDLPEGGIGTFKPSFMNIGISYAKAFSNAIYGGL
                     VLKVVSESIADNRAQGVAIDAGIQYLTGDNENIHFGIALRNIGPTMKFTGDGLSFRGN
                     MNNSDVLMTVEQRSVAFQLPALLNIGAAYDFIINDAHTITFAGSFTSMSFGKDVYAGG
                     LQYNFNNYLMLRGGMQYEKGLFDSDERTSAYTGPTAGITIQVPLNKDKGSTFSLDYSY
                     RTSNPFQGTHCIGARINL"
     gene            22826..23296
                     /gene="greA"
                     /locus_tag="PKY63_00060"
     CDS             22826..23296
                     /gene="greA"
                     /locus_tag="PKY63_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPE99031.1"
                     /translation="MAKQTYITKEGLEKLKAELDQLMNVERPKISKQIAEARDKGDLS
                     ENAEYDAAKDAQGMLELKISQMQEIVSNSRIIDESKLDNSKARILSTVTIENKKTKQK
                     MTYTLVPDQEADLKAGKLSVNSPIAQALLGKKVGDSADVKVPAGVISLKLLDIK"
     gene            23301..23696
                     /locus_tag="PKY63_00065"
     CDS             23301..23696
                     /locus_tag="PKY63_00065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPE99032.1"
                     /translation="MASVFSKIIAREIPAHIVAESACCMAFLDISPLNYGHTLVVPKT
                     EVDYLFDLEPQMLSDLMVFAQRVSRAIDQCGWGKRVGVAVIGLEVPHAHIHLVPINGL
                     HDLEFSNEKLKLSNEELTAIAEQIRSKFI"
     gene            complement(23689..24255)
                     /gene="ruvC"
                     /locus_tag="PKY63_00070"
     CDS             complement(23689..24255)
                     /gene="ruvC"
                     /locus_tag="PKY63_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPE99033.1"
                     /translation="MNSEQLILGIDPGTNIMGYCLLKYDGHNYAIVTIGCLRLSKYET
                     HIDKLRGIVEFTSNLLNTHKPQVMAIEEPFFGKNVQSMLKLGRAQGSVMSVAMQHGLE
                     IVEITPRRVKQAITGKGSASKEQVAAMVANITGYDISKLNTDATDAAAIAISYTLQKI
                     KTTSAPTRKKNSNSWDQFLRENPNKILK"
     gene            24335..25324
                     /locus_tag="PKY63_00075"
     CDS             24335..25324
                     /locus_tag="PKY63_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99034.1"
                     /translation="MNKKAINIVLSIIITSLILVFIGYLIHDRLQSIDIGYSLSILFE
                     SGRSVVLLVLVFLLMPLNLFCEAFKWKTLADSIERISLMRAFSGILAGITAGLLLPNR
                     VGEFAGKALSLSLSQFWKGSVLAILTSMAQLMITLIMGFLAIIYFGPQLNYFFGIRIS
                     FLPVAISVAGIALLLFLFFNIQAVALLFKRWEKLHTIVAVLSSSGLSIKLLILFVSAL
                     RYMVFAGQNLLLLALFEVNIPWVDSFFLIALMYFTLSAIPTFVLTDFPARSSVMLLLF
                     IAWFDISGLTIPYALEAKIIVTSFLIWFINLIIPAIPGLFFLNRFSVLRKANR"
     gene            25321..26430
                     /locus_tag="PKY63_00080"
     CDS             25321..26430
                     /locus_tag="PKY63_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99035.1"
                     /translation="MIVIVVTIGFIAFFAFIEALFAIGIMADSNALLRKKNIEYSVVV
                     AVRNEEEWIESCIQSLFNQSVQPSAIIVVDDHSDDKTFCIVHQMIAQNPLLVLLSCDH
                     DETGKRAALMKGISAVQTEVVLTIDGDCMARRDWALNMLSKFSENKKLVSGPVITLSK
                     RCLNMPEYAESLFLMAVGAGAASQGLMFQASGANMLFLKSDFVDFYKSASGAEFRCGD
                     DVFFLQYIQKQYGRKASGFCSNSGAVVETHSSAGIKEWIFQRVRWASKSRGYSDWIPF
                     AAGILVLFSSSALVAAFILLIAQPEFSFFLLPVILVKVMGDVLLLFSSAFAWKAPVRV
                     LSFFMLPLFYPLFLMSVGSAMIFQKNKIWKGRAIS"
     gene            complement(26423..27100)
                     /locus_tag="PKY63_00085"
     CDS             complement(26423..27100)
                     /locus_tag="PKY63_00085"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPE99036.1"
                     /translation="MPTPFHIIVVAGGSGKRMNSAIPKQFLKINETPVIAVTINRLYK
                     IVPNSRITIVIAERDLPHWKECHKFIPFHDSINIAFGGPERFHSVKSGLAFVHADEVI
                     AIHDAVRPLFTANVIHQGFMVAEKSGSAIPVIPLSESIREINGALSKAANREQFRLCQ
                     TPQFFQATLLLDAYRQSYIQSFTDDASVVEAAGYPIRLIEGNPENIKITTPADLKYAE
                     ALLPHIN"
     gene            complement(27100..28149)
                     /gene="queA"
                     /locus_tag="PKY63_00090"
     CDS             complement(27100..28149)
                     /gene="queA"
                     /locus_tag="PKY63_00090"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPE99037.1"
                     /translation="MKLSDFKFDLPQDLIANFPSKNRDESRLMVLDRKAGTIQHKKFN
                     DILKYFGDGDLMIINNTKVFPARLYGRKEKTGAVIEVFLLRELNAESKLWDVLVDPAR
                     KIRIGNKLYFGENDELVAEVIDNTTSRGRTLRFLTDMEHDKFKELLKNMGEPPLPKLI
                     NRPVIEDDKDRYQTIYAKHEGAVAAPTAGLHFSRELMKRLEIKGVDFAEITLHAGLGN
                     FRAIEVEDLSKHKMDSEEFFIDERTADKVNEVKERKGKICAVGTTSMRAIESSVTING
                     YLKPFKGWTNKFIFPPYDFSVADAMVTNFHLPMSSLMMMVAAFAGYPLLMDAYKTAVK
                     EKYKFFTYGDAMLII"
     gene            28339..29229
                     /locus_tag="PKY63_00095"
     CDS             28339..29229
                     /locus_tag="PKY63_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPE99038.1"
                     /translation="MAYITKEKFLSAEWFRVYLLILIGSFIMAAGFVLFISPYKLAPG
                     GVYGIAIVLHYLINTPIGLIGIAIDIPLTIIAIKILGPKFGVKTVAGFVLLSGWISLL
                     EFTWGYKPLVEGDPLLSSIFGGLLIGFGLGLIFKSKASSGGSDIIAMILNKYTKIPVG
                     QLLIAVDAAIVMISLVAFDDWKIPLYSWIVIFITGKVIDAVIQGVSYDKTCMIITDKP
                     EEVSNKILVDLKRGGTFIRAKGMYSGQEKDMIYTVVNRREVAILQDYIRQIDPNAFMS
                     VIDANEIVGNGFKPFSEKGQ"
     gene            complement(29238..31586)
                     /locus_tag="PKY63_00100"
     CDS             complement(29238..31586)
                     /locus_tag="PKY63_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPE99039.1"
                     /translation="MKTFFIAILILFISAAASAQQKSPGSDSIDVIRYSINIDTLDIS
                     AKQLYGYTDIYFQPKVNNLQYIPLDLLELNVDQVYLESTLNTTWTYNDTLLMIQSPVA
                     LAGNDTFHLKIFYHGSPVVDPSGWGGFYFQSPYAYNLGVGFEDNPHNYGRVWFPCIDD
                     FTDKAFYEIIVRTDTNQMAVCGGMLTDSTHIENADSIAWTWTMAEPIPTYLASIAVGP
                     YVCVTDTFNSINGNIPIDIYVSSSYVSKVPGTFIHLKDILATFENRFGPYGWERVGYV
                     GVPFNSGAMEHTTNIALPNATITGNTSYESLIYHELSHHWFGDLVTCATAEEMWINEG
                     WAVFCEFITEESLYGTAAMQDYIRTMLASVLRFAHVDDGGFYPVGNVPHHITYGTTVY
                     DKGALVAHTLRNYIGDSLFFPALKQYFDSLKYGNATIIQMRDILSAETGMDLTDFFDA
                     WVLREGFPHFDVDSMHVVPNGTGSTVTVFLKQKLYGTTQYANSNRLELTFLDSLWNKH
                     TVTTSFSGQNGTDVFNVPFIPTLVITDLNEKTADAIVSYNSVISAPATVSFDYAYSDI
                     FVNNLTDSIFVRAEHHKVAPDPVVSNPDIFRISESRYWRISGIVPAGTDYQLRFDYNR
                     TNIFFDSGLLPQNASTDSLILVYRPGPGYDWQLFPFTKTGNDFSGFLKTPYGLLGEYA
                     LAVGQPNQSGIAEPGSESDLLIFPNPCSDLLQIANIPQNCTEITICDMSGKIMLRQHL
                     SNHETSTTIDIRSIAAGEYIVNVIGRNGQMKAKSTLTVIGTK"
     gene            31924..33342
                     /locus_tag="PKY63_00105"
     CDS             31924..33342
                     /locus_tag="PKY63_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE99040.1"
                     /translation="MKRLLFFILLLAAVAAEGQTVWTLESCIQYARENNLTIRLQKLN
                     SEIAKLSLTRDKAAFLPGVSAGATHSYNYGRTVDMYTNEFATDRVRSNNFYIGANITL
                     FNGFNLINSYKKSQLEAEAAILDAQQTSDDISLSIATAYLQVLYSMELVNNAQSQLDI
                     TLLQVDKNRKMYAAGSVSQGALLTIEAQAASEESQLVSAQNQLEMSYLTLAQMLDLKD
                     MSGFQIAVPDISTLDTSSFLLMNPADVYAFAEKNQPGLMAAQYRVQIAERNLGIARAG
                     RYPQLTLNGSLGTGYSGASRRMTGYNVNGVDTIGFTSESLTEYVMMPSLDYTYETTPF
                     QDQINDNINKSIGLQLTIPIFSRLNTTLAVRQAKIGIESATINFEQEKLNLQKTIQQA
                     YADASGAYRKYSATKKQVAALEESFKYMDQRFSVGLATPIDYSDAKNKLATARSEMLQ
                     AKYEFIFRTKILDYYLGREIRL"
     gene            33361..34698
                     /locus_tag="PKY63_00110"
     CDS             33361..34698
                     /locus_tag="PKY63_00110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE99041.1"
                     /translation="MALKKKNKRIIIWSIVGLAVLLIILRKAGVIGGVETIQVAVENV
                     TQRDITETVSASGKIQPVREVKLSPDVSGEIIELLVKEGDRVKEGQVLARVKPDIYQA
                     NYEQLAANVNSQKANLSNSEARLAQTEAQFVNTEASYNRNKTLFDQGVISKAEFDAAQ
                     AQYDVAKAEVQASKQTVNAARFNVYSAQASMSEASKNLSRTTIVAPVDGTISTLNVEV
                     GERVAGASQFSSGTEILRIADMAEMEVMASVNENEVVRLSLGDTAIVDVDAYPNKKFK
                     GIVTEIGNSANVSGISADQVSNFDVTIQILSSSYADMIPKGAVDYSPFRPGMSASVEI
                     QTEKVKQVLSVPVQSVTTRDESELMVVDDKKKTDEKDNHDGITEFVFVIENGIAKRRT
                     VKTGVQDNEYIQILEGLKKDEVVIVAPYSAIAKKLQGEEKVEVVDRKNLFEVN"
     gene            34695..35390
                     /gene="tsaB"
                     /locus_tag="PKY63_00115"
     CDS             34695..35390
                     /gene="tsaB"
                     /locus_tag="PKY63_00115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPE99042.1"
                     /translation="MSLILHIETATQVCSAALSHDGMLMAEAVAETEMSHASTLMPLI
                     DELFKKQNIDLKDLCAVSVSSGPGSYTGLRIGASTAKGICYSLGIPLIAVGSLHSAAS
                     GMKEISNAKGTDLLIPMIDARRMEAFAAIYDASLKELRAPHPWILDQSSLDEFSGRKI
                     FLAGTGADKLKALYSSFSNVVFLNENVHRASFACSIAFDKFQHSAFEDTAYFEPDYGK
                     DFVPGKPVIKGLK"
     gene            35403..36386
                     /locus_tag="PKY63_00120"
     CDS             35403..36386
                     /locus_tag="PKY63_00120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99043.1"
                     /translation="MTPYFSVIVPVYNRPDEVDELLDSLVKQTNKDFEVLIVEDGSTL
                     KCEDVCNKYVDKLDLKYLFKPNSGRSETRNYGMERANGEYFIIFDSDVILPSNYFEIV
                     RKNLEADYIDCYGGPDSADDSFSIVQKAINYSMTSIMTTGGIRGATKNVDKFSPRSFN
                     MGMSKEVFQKVGGYKNMIGEDIDLSFRIKQAGFKTRLFRDAYVFHKRRVSFKKFYRQV
                     NTFGKGRVLLSKIHPGTLKAVHLLPMFFVLGNLALAALAAIFLNPWFLLPLGVYVIAV
                     FFESLIKNKKLSIAILSIVASYWQLFGYGTGFMGELITGKAGKSSQEKLYK"
     gene            complement(36388..37164)
                     /locus_tag="PKY63_00125"
     CDS             complement(36388..37164)
                     /locus_tag="PKY63_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPE99044.1"
                     /translation="MAVVSIITITYNAEKVLEKTMQSVLQQKNGSVEYVIVDGNSKDK
                     TVEIIQRFEKEIAEGKYPAVKSSDFRYISEPDKGLYDAMNKGMQMATGDFVWFINAGD
                     KIFDEHTVEKIENALRLNPSSDVVYGQSLMIGENDQPLGERHKIAPANLKKKHLLRGL
                     VVCHQSILVRKSIAPLYDLQYRISADYDWTAKVLAVSKHNLFIDDYISRFMISGLSAQ
                     HRKKSWKERFCIMKKHFGLCRTLWAHFIIIMKYPFTRKYN"
     gene            complement(37167..38093)
                     /locus_tag="PKY63_00130"
     CDS             complement(37167..38093)
                     /locus_tag="PKY63_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPE99045.1"
                     /translation="MMELNEKIYVAGHTGLVGSAIVRSLRKHGFNDIIHRDITELDLR
                     NQKAVNDFFASEKPDYVILAAAKVGGIHANNTYPAEFIYDNMMIEFNVIHAAYLNQVK
                     KLLFLGSSCIYPKMAPQPIREEFLLTGALEPTNEAYAIAKISGLELCKFYRRQYGCDF
                     ISAMPTNLYGINDNFDLNSSHVLPAMIRKFHEAKIENMPEVVLWGTGKPLREFLYVDD
                     LADALVHLMQHYSEEIHVNIGTGEDLSINELALTVKSVVGYEGKMVYDTTRPDGTPRK
                     LLDVSRLHASGWKHKTTLKEGIEKVYQWYLNK"
     gene            complement(38095..39159)
                     /gene="gmd"
                     /locus_tag="PKY63_00135"
     CDS             complement(38095..39159)
                     /gene="gmd"
                     /locus_tag="PKY63_00135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPE99046.1"
                     /translation="MKKTALITGITGQDGSYLAEFLLEKGYDVHGLMRRASYFNTGRI
                     EHLYFDEWIEDMKHQKKIELHYGDLTDSSSIIRIIQSVQPDEIYNLAAQSHVKVSFEV
                     PEYTAETDAIGTLRVLEAVRILGLEKKTKIYQASTSELYGKVQEVPQSENTPFYPRSP
                     YGVAKLYGFWITKNYREAYNIFAANGILFNHESERRGETFVTRKITLAAARITHGLQK
                     KLYLGNLNAMRDWGYAKDYIECMWLILQHNTAEDFVIATGQMHSVREFCTLAFAEAGI
                     ELRWEGENENEKGIDVKTGNTLVEVDPKYYRPTEVEQLQGDPSKARKTLGWNPTKTSF
                     EELVRIMVKYDLEFVKKNLE"
     gene            complement(39164..41647)
                     /locus_tag="PKY63_00140"
     CDS             complement(39164..41647)
                     /locus_tag="PKY63_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99047.1"
                     /translation="MKRINIRLWFPHLIAVLIFIALSVAYFSPVVFDNKDLPQGDVAS
                     AQAWGKDIRDHYEKTGEYAYWSNTMFGGMPANYTYAPPMTNIFKDLSIILRLNLPGLH
                     VGIVFLYLIGFYILLMAFNVKPWLAIVGSIAYAFASYNLIIIDAGHVNKGLAMATMAP
                     VIGGIILTYQKKYLIGILTTLIFTGIHIYYNHQQISYYLLLIIAIMFVTYFIYAIKEK
                     AIKHFLKVSAILLIVAGIAVLPSIGRMITIMDYTKETMRGGAVLKNNPKGQKEGSGLD
                     IDYAYQWSYGIGETWTLLIPNLYGASSHYNIGNESECYKTLKANGVGGAQGKNFCKYA
                     PTYWGAQPFTSGPVYAGAIVCFLFVLGLILVKGKEKWWLLAATILSVMLAWGKNLGGF
                     NQFIFDNLPMYNKFRTPAMALTIAGVTMAILAILAIKEFIEQYKDENKKEALLKKLYI
                     AGGITGGLTLIFAVLGGGLFDFSSASDANYPDWLIDSLQADRKSMMVNDAWRSFAFIF
                     LAFGLMWAFLKYRFKNNYLIIGLGALILIDLWVVDRRFLGNDNFVPKRKAKTFQMTEA
                     DKIILEDKDPNYRVLNLASSTFNESNTSYYHKSIGGYSPAKLRRYQDIIDYHLSEKIN
                     VNVLNMLNTRYVIVPNRETGGTGVQKNPDALGNAWFVDTLKWVNGPDEEIVAITDFNP
                     RTTAVIDQVWKDKLSSPEALQTTADSSAAIVMMDYSPGAISYKTTNTKNQLAVFSEVF
                     YKTWKATIDGKDVPIIRVNYILRGLQVPAGNHVIQFTCKDELYIKGQKISLWGSVIAG
                     LALISIIGLLIWKWIRNNNKADNSTESIE"
     gene            complement(41844..42503)
                     /locus_tag="PKY63_00145"
     CDS             complement(41844..42503)
                     /locus_tag="PKY63_00145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HPE99048.1"
                     /translation="MFGKIKDLFTTFFEFMRFRENNVFLSYIRSGSKRIKKGKHVKIA
                     IPGHIRDAEVGDYTYISMNSWINNTKIGKFCSIGPNLLCGWGVHPTNGISTAPMFYST
                     ARQNGISLCTTDKLNERKTITIGNDVFIGANVTILDGVSIGDGAIIGAGAVVSKDIPP
                     YAIAVGVPVQIVKYRFTEEQIQNLQKIQWWNFDPSRLLEIEQHFFNIEEFIESNLNKQ
                     G"
     gene            complement(42513..43370)
                     /locus_tag="PKY63_00150"
     CDS             complement(42513..43370)
                     /locus_tag="PKY63_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99049.1"
                     /translation="MYPKQLQWFYNSIDAFFSLFRLLWISRFTKTKKINKKHSHCIVL
                     GNGPSLNNTIKSLNHISETHDLIAINFMGTTDIFAELKPETYVLCDPAFWFDVKQEKQ
                     KEKVLDFYQHLTSQVTWEMQLYLPWQACKEKEINQILKKNNNITICYYNKTKVEGFRF
                     LKKFAYRKQLGMPRAQNVLVAALMLAIYSGYSEIYLIGSENDWLKNLWVDEKNKLRLN
                     DFHFYDKEDAAKNARTMEFDLATMLSFLYFMFKGYHEIEQYSKTTKSTVFNATPESFI
                     DAFKRKSVS"
     gene            complement(43508..44134)
                     /locus_tag="PKY63_00155"
     CDS             complement(43508..44134)
                     /locus_tag="PKY63_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99050.1"
                     /translation="MKKLLPAALVLAVALIFTGCKKDDPEPDNNTPTTPTSSVFTFFK
                     TGATWTYDTYDSDAGSPHFNQTFTINSINAQKYANVTWNVAGMYTITQEWYADDTKFS
                     MLCSQGGGTMLVFCDANPAVDEFWAETWTDTSGTVSDSCRIVALNETVTVPAGTFTNC
                     IKILETTSEDPVYYKYIWFNLSTGVIKTEGTTTEDYPTIIYEDLSSHN"
     gene            complement(44156..45463)
                     /locus_tag="PKY63_00160"
     CDS             complement(44156..45463)
                     /locus_tag="PKY63_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99051.1"
                     /translation="MKQFLILLTALLISSISFAQIDIGGKIKNKAQEKLEQKTDESID
                     KGFDKTEEGINGVFKKKNKDKDKNAEDGETEENIEGENTEESENVEENSSSENGENNV
                     KLESYSVYDFIPGDKILFFEDFSQDAIGDFPALWTSNSSGEVKTVNIAPGKWFHLNGE
                     DATYCFSKDIAFPDNYIFEFDIIPDADYSRGIVLSLYQTDPENVREIEDALYPGLYGL
                     RITLAPDGWESKGYGESGKDWLEGNASKNPIVKEKVNHVIIWVQKRRVRIYHQGAKVL
                     DVPTNIYAEGKFNRFRFFGWDRNSHPFVTNLKITTASPDMRSKLLTEGKLISYGIYFD
                     SGKDVVKSESQGSLNEIANILKENPDVRIKIVGHTDSDGDDAMNLDLSKRRAANVKTK
                     LVNDFGIDASRIETDGLGESQPIGPNDNPENKSKNRRVEFIKL"
     gene            45795..47120
                     /locus_tag="PKY63_00165"
     CDS             45795..47120
                     /locus_tag="PKY63_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99052.1"
                     /translation="MNTKFLVLLLGIGFLMFSCGDDKKAQIRKVMKPVASVDLGTQVF
                     IIDPTKDTLLNMESGSSVFIPANSLVGADNKPVKGKAELKYEEYLNQAEVILSGIPMT
                     YDSAGETHYFESAGMFTIDANTPAGEKLKIADGKSVTVSQVSPWADLTSGYNFYQFDT
                     VAGKWRYLTTQIAEPAASEPPVIETGKDADVKVKKSVRTVDDFVFDINVNYTVYKELA
                     DMKDIMWKYSGNKEYPDPEKEQWIFDYNWPETQILRDESQPGAYVIVLKSTQKSFKTS
                     VVPVPLEAMEEEDVIARADKSMEKLGEQTAMMVENSNSYRRTMEVSSLGICNWDLIYR
                     FIVPKRLKIRFFADGDIFKEDVRIYQVNIDRNVTIECLPENAHYNCILSSGEKIMYVA
                     VSPEGQAYVNKETAILMNASEDEVIDFDLHKTAKTINSKKDLNELIAGV"
     gene            47132..50689
                     /locus_tag="PKY63_00170"
     CDS             47132..50689
                     /locus_tag="PKY63_00170"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPE99053.1"
                     /translation="MRLLQVFLLILLFNAGFCQESGPLFIVRQDQLYGLIDSSGSVVL
                     EPQFTAISYDPQFRIFIVTGQSGKAAFASSQNAFITGFYDEVQAVSLYYFIVTRNGLC
                     GVVDRSGKMIVPIQYSSIRRSDSDRFFCSNNASVAVYNNNGSLVVNIPSEEIIEYCGY
                     YIFKNRDYRFGVVSVSGEKMIEDVYSNFLTGDGLIELRSEDSTAILHTSSGRVVHGAA
                     GCSFRFIKDASRNNLATPFYLLANGSSRQWHSVYSHKNLDVDLSEAVIYATALSDLYL
                     VNTTFEWLIDNNGVSVTDEKYEDIFYFRDSLFVIENNGLRGLFKPGTGVILQPIYKSF
                     RSLTDTITGNRWTLALSNPDGYRFLDQNFREINDQLYFAVNLVHGRGIIVNISLSEVI
                     LLDTDGRRVGNAVYSAIQPLSDGFWKVYRNGFCGVMDSQAKLLTEICYDDITMHNSRL
                     KCVREDRIEYYRWNGSSFEPLINFSNFRQLDVTGTQLLSLIGTRSSQLALVWRRDSVL
                     KKFGLYDVFNKRFQVSPVYDYAKMDQLRDISLTGIFGDTSYFRLGPLTFMGRIRFGVI
                     DHKTGQVIAPNEFMFIYFNQFQRHFSESNSDLVTPYYNSDRNFAVAIDTDGNWRTISF
                     TGTTYNPDVQFIDVSSGTRKVWRFDSCYVSNKRKRITDKKICNVNAAFAPLLSFLKPA
                     DAYTEAAMKDKRLSVFAPDADSLVLQKGLASRLDEGLDDIPLVYFRPLFVYKYHYPSI
                     LEYRNRLFCNVLIFESERRFFCIDSTGYVITDTAYSRIWPFSEDLAMVKMGSRYGFID
                     PHGALIIENKYRKASSFSEGKAAVSIKGGRYGYIGTDGAWIIEPAYREALAFSEGLAP
                     VKPGNLFGYVDESGKMILKPAWLRANSFINGTAVVFNKRGFGLIDKSGNELLKCRYNR
                     ITPPDTNGNRMIVRKASQLVNASGDIITKKSRIISDAGNGYYSVKKVVGFSLIRSDGS
                     EVAHYNSDRPLLVGDEKVIVTKRNRLYYYDLAANQLLGPYYKAAPFVMNRALVSIRDE
                     TMVIDSSGKILKILYRKRMSFVTSFDENGLAVVRAGNTFMLIDETGAEFYNGTIKPRY
                     AGNGVYILFHDGYFLYNHRIRTSYPFSKWSLISMPSCGRFVTGVNGKFGFSDVYGQYH
                     VRPQYTRIERVSEGIYRVYYGDKTGYVRYDGEIIWELSR"
     gene            complement(50737..51678)
                     /locus_tag="PKY63_00175"
     CDS             complement(50737..51678)
                     /locus_tag="PKY63_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPE99054.1"
                     /translation="MKLPQKRFICIGETVYDILFKEDKPVEGTPGGAMLNTSVSLGRL
                     GENVVLVGDYAKDAIGRLIEKFLVRNQVSLDYIKSYNNGLSRIAIAVLNDNNDAEYSF
                     YKIRKEGQAELIYPEPDKNDLVMFGSFYGIKEEIREGLTAFLHVCRNVGSIVLYDPNF
                     RKNHMKIKDQVMPMISENMRLAHITKGSDEDFNNILGTSDPKIVYDFVQTQGGHVLIM
                     TANKHGVTLCTPQIQKHYPVKDIPVISTIGAGDTFNAGLLYALSHTHISPADLETLPE
                     KNWDKIIRTAIQFAEHVCMSYENYLSWDFAREVANGH"
     gene            complement(51691..52428)
                     /locus_tag="PKY63_00180"
     CDS             complement(51691..52428)
                     /locus_tag="PKY63_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPE99055.1"
                     /translation="MKIKTAIPNIFTAGNMLAGLCAIVATLGSWYYIEYALYCILIAA
                     AFDLMDGMVARMLNAQSSFGKEFDSLCDVVSFGVAPAVVTIRTMQQYSEPCNDLFCNQ
                     TMFFAFPLLFALAAAIRLAWFNTDERQTKSFLGLPSPAAGMALACISLYWAELYGIDN
                     AIILLSIASFTFAALMLIPFPLLSLKLHDQKSIALGIILLMIAVPLFIFLDLAAIAPL
                     IIVYIFISPLIGYIAKIFAKYNSTNPS"
     gene            52566..54341
                     /locus_tag="PKY63_00185"
     CDS             52566..54341
                     /locus_tag="PKY63_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99056.1"
                     /translation="MGKFFILVILLLALMPAEGFTQCGTTDHNGADWIITSSDTLGGT
                     HINIGKFEVAIGICVSVNPACKFFAVEADTILIYGSIDGDAKGDAGGNGGTGGLYCNG
                     SGVPGSGGHAGLAGYGTGGGIPGAAAGTGGTIEQICGGFLCAGNQDGFNGGGGGAGGG
                     SGGSYGASGGAGGYGAYGSGFTGAEGGDYGSGGTTSSAYGTADGYDIAWGSGGAGGGG
                     GGGGWATGSAGGNGGRGGGMVSLKAAHKLIMSGNISCNGSDGGNGGNGGGESDDNGFD
                     CSTTGYNACGVCSESVYDAAGGAGGAAGGGSGGGIYLESNGTATITGTITAIGGAGGT
                     AGIPSSSMGTCYDNARGGGGGSGGRVKILLNPCITNLISPVCNISGGTGGNGVVTGYV
                     GTSGTFEDDLIAQEYIALAGGSIADIDTTFCDFGDVPLISSVAAASGGMGTYNYQWQY
                     STTDSLSGFTNYPGQTSLTMDPSLISVTTWFRRKVSSGSCIEYSNVIKAEVVDCSGFE
                     DNSGLLFSVYPVPNAGAFSVQLPLVMQKRAEGYIYNSRGELVSEFSITAGEQKTDIQL
                     NVAPGLYLIVLYTDQNIGLKKITIE"
     gene            complement(54408..55736)
                     /locus_tag="PKY63_00190"
     CDS             complement(54408..55736)
                     /locus_tag="PKY63_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPE99057.1"
                     /translation="MAKTTQKQKKIFVLDTSVILYNHSAIYSFEDNDVAMPISVLEEL
                     DTFKKGNDTKNYEAREFIRILDKMSDNQPIKDWIKIERAKGMFTVIMDETSNPDATKL
                     YHSDKVDHRIINVAIKLSQKYKDRKVILVSKDINLRLKAKALNIIAEDFETGKVKDVE
                     GLYTGMTLVEGLTKDIIDSIYQNGFCLPGDVGIKKPIPNHYFILRNTSTSVLAYYNPI
                     TGNIEKLEKKPAYRIMPRNAEQVFALHAITAPDIKLVTLQGVAGTGKTLLALAGALEQ
                     KKLFKQVYLARPIVPLSNKDIGFLPGDIKSKLNPYMEPLFDNLKFIKNQYGDKEATQI
                     DELLEKEKLVITPLAYIRGRSLSNICFIVDEAQNLTPHEVKTIITRAGEGTKIIFTGD
                     IHQIDTPYLDSQSNGLSYLIDKIKHHALYAHVKLEKGERSELANLANDLL"
     gene            55916..56926
                     /locus_tag="PKY63_00195"
     CDS             55916..56926
                     /locus_tag="PKY63_00195"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPE99058.1"
                     /translation="MKKIKVGINGYGRIGKMVFRILLNHPEIEIAGINDPMPENSLLY
                     LMKYDTVFGILNETITAGDDSVMVGKRHIPVSRVVSPAEIPWKKWGVEVVIDSSGRNK
                     DRESLQQHIVAGAQKVILTCPAVSPVDKTVIIGVNEHTLAAGDHIVSNASCTANCVAP
                     LLKIISDSFGIETGFLNTVHPYTNNQQLIDSPHKDLRRGRNAGVNIIPTSSTAIKCVL
                     EVLPELGDRFSGIATRVPVPDGALTELVLRLKSIVTITELNIAVKHSSKTSMKGIVSY
                     INDPVVSSDIIGNPHSCVFDSLATKVINGDTVQLLSWYDNEFGYSNRVVDLIGLMAKF
                     IR"
     gene            56985..61088
                     /locus_tag="PKY63_00200"
     CDS             56985..61088
                     /locus_tag="PKY63_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPE99059.1"
                     /translation="MDKKFYSKALEVNLNQTKQGFIDLPADHRWLISLSEKHWGINKY
                     TTEFFNELHHRYPNYEHLSNELRKIALGDFWLYSGHNESLRALGTINSVLSELMVKPV
                     SGKILEQLVQTRVDFCASVAALPEPDAGLIRESIQSLQNLLSDHVEIVLSNSVYIRQK
                     WNLLADLKVVSKEYFDFLKVLCRRNLDYWHVTMKMDQWLNARRNSFSEFSNDVLSEWG
                     STAYENYSKRIDAAKSVQDLSDNVPLYSDFANHFRRLADRLETPQDRFQFIVFLLSTP
                     GMSHLSHFLLVDLNVVLRTIFDNPDDEQMIPFLTEIFSLFGEIMDNNPSTVLDCIKTI
                     GLKLSSVDNPKLLKIFQRKCLELGFIRPGGQYMTSEWRMNINPYHVKNIRVWLELFES
                     APEKFQTLLAGLTANLKLGGIFIFDTDLFQKDVSQLLNSDIKSTFKLSKQLCRIFPVY
                     FNEIGAEGDLRDVTTQMDEISHRQDRLIHFLRKQIHIESNNTHIALTVNIFRFWIHKD
                     KTQLRDFLPEDVYLAVEPEGQFVKHLHAMIMEISEQHNTDPYQLIHQDPELLSQWIDA
                     CGDFPVNEKKRLKFISRLNFILEEKYSFNTVDICKYLRRFNFLASTDIDILEDLLQNG
                     RKEQAVKQIYKFMEQLNGIILSDKKSEGWENIYYKRHVAFGIPSMYGEYHERRFESLG
                     LIFKLERLAAELTEELRQDLNLHVITANSLHKISKILELYRQGIKLDGISIKGFDLNL
                     KMFIFSLQSQSFSLQQHMNLFRFMSESIREIITRYFFRPYDEPLKIVIPQIFDCNELN
                     ESKRMQFIHARSEEFYRGMLSSAFLLQSLDGFVAEVSDAMHKLADNNPPQMVRDIMSL
                     NTDMMLSPFYSRTPAMDNQVFLGSKAYFLKTLYLNGFPVPPGFVITTEVFRRIDTILQ
                     HDALTAAVDNLILQNVRKLEKITGKKFGSKSNPLLLSVRSGTAISMPGAMNTFLNVGI
                     NEEIAEGLSKQENYAWTSWDCYRRFLQTWGMSYGIDRDVFDQIMIDFKTRYHVQQKVQ
                     FKPEQMREMAICYKELILKQGIPIEEDPVKQLKQTILNVFNSWSSPKAVVYRKILQIA
                     DEWGTAVIVQKMVLGNLNYTSGTGVLFTHAPHVSKPGIFLFGDYSLVSQGEDIVGGLV
                     NPRALSSRQPNDDNPALSLQVLFPDIFERLLKLATHMIEDNGFSHQEIEFTFESQNPD
                     DLYILQTRDQDLTSQSKPDVFFEPVAEEHLLGRGIGIGGGAMNGIVVFDDNDIRHFRK
                     SRPDCNLILVRPDTVPEDIGLIFETNGLVTARGGATSHAAVTAVRLGKICVVNVASLV
                     VNDRKKQFSVGSVVVSAGDEIAIDGTIGCVYKGNFKLDVFKNQSLLN"
     gene            61108..62358
                     /locus_tag="PKY63_00205"
     CDS             61108..62358
                     /locus_tag="PKY63_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPE99060.1"
                     /translation="MEISVKNKRLLGINSLGRIGKLTLWNQLATRNFDGIVVNVGREV
                     GKTTDDLLQVLETDTTYGQLGKFLHGVAGKPLVSKVVDEANMLFEIDGMPVKVLRSER
                     NPSGIPWRDEGVQLVIDCTGQFLDPVVPPDAPKGSLRGHLVGGARKVIASAPFKIKDK
                     SARMPEDALMMVFGVNHLNYNPSVHHVISAASCTTTGLSHMMKPLLEDAETSKILTAS
                     MSTVHSATNTQSVLDAVPKAAASDLRKSRSVFNNIILSTTGAAKALESILPDIQKIGF
                     MADSVRIPTTTVSLITLNITFNSGLDESGNPLINRNYINRIYKNAATGAQKDLLIFSE
                     RQNVSADMMGFRAAAVIEGCETHTRTGFVNLPASMFEAYGMKNASDINMPVTHAKIFG
                     WYDNEFGSYVTMLSKLAAFINSKL"
     gene            complement(62409..63737)
                     /gene="miaB"
                     /locus_tag="PKY63_00210"
     CDS             complement(62409..63737)
                     /gene="miaB"
                     /locus_tag="PKY63_00210"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPE99061.1"
                     /translation="MTEKNKKLYIETYGCQMNVADSEVVASILGEEGYDICNDATEAD
                     LILVNTCSIRENAETKVLARGRELQTLKKKKPGLRIGIIGCMAERMAESIFEKLPGID
                     LLAGPDSYKKLPDLLRETETHKKASDVILSTTETYEDIDPEHYQTGEITAFVSIMRGC
                     NNFCSYCVVPYTRGRERSRPVQSILHETELLHEKKFREITLLGQNVNSYHFTEKSIEY
                     HFPKLLDKVASEFPSMRVRFATSHPKDLSENLVKVMAKHDNICKSIHLPVQSGSDAVL
                     QRMNRKYTAADYLKKIELLRQYMPGIAISTDIITGFCGETDEDHQATLNMMKAADYYY
                     AFMFKYSRREGTSAAKIPDDVPEKTKSQRLTEIINLQQELSLHHNQRDIGKTFEVLVE
                     GFSKRSDKHMFGRTGQNKVVVFDAENILPGTLVNTIITDCTAATLKGKMV"
     gene            63915..66362
                     /gene="topA"
                     /locus_tag="PKY63_00215"
     CDS             63915..66362
                     /gene="topA"
                     /locus_tag="PKY63_00215"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPE99062.1"
                     /translation="MGKNLVIVESPAKAKTIEKFLGKDYQVLSSYGHVRDLQKEDLSI
                     DVNNGFKPLYIILPDKQKIISELKKLSKQAETTWLATDEDREGEAIAWHLAEALNLDV
                     KKTKRIVFHEITKTAILEAIENPRNLDLNLVDAQQARRVLDRLVGYRLSPLLWKKVKP
                     ALSAGRVQSVAVRLIVEREDEINQFTSTSEFRVNGVFEAGKDSVNAELNERFENERDS
                     MMFLDKIKDSIFSIGKIEVKPGTRNPAPPFTTSTLQQEAARKLGFSVSQTMTIAQQLY
                     EAGLITYMRTDSVNLSKLAIGSIKNEIEKSFGKEYSKPRQFATKSKGAQEAHEAIRPT
                     YAENREIEGPAAQKKLYDLIWKRTVASQMGEAKIERTSVQIDISGVPQYFVARGEVIV
                     FDGFLKVYEESRDDDASDDDKGQLPKLAEGQKLSQQKIIAEQRFSQQPPRYSEASLVK
                     KLEDLGIGRPSTYAPTISTIQRREYVIKESRQPVERKYISLLLAKGAIKRTVETEKFG
                     SEKNKLFPTDLGIIVTKFLVEHFENIVDYNFTATIEKDFDEIADGKKKWNALIANFYK
                     DFDPQVINIDKTTGKYHGERLLGIDPESKKNVYVKLGPFGPMAQIGESTDEEKPRFAG
                     LLRGQTIDGVTLEEALLLFRFPKIVGEHEGKEVVVSVGRFGPYVRFDGKFYSIPRDIN
                     PTQVNLASALEIIANKQEKDRNSVLKIFKEEPELQIVKGRFGPYIKFKGKNYPLGKGR
                     KYDALTVTEILEIVKEHPEKPSRKQKTVKAAKTKKTAATEKAEPKKKATAKTTKAKTA
                     TKKTTAKKTSVSKSKKK"
     gene            66366..67535
                     /locus_tag="PKY63_00220"
     CDS             66366..67535
                     /locus_tag="PKY63_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPE99063.1"
                     /translation="MNELQLYFDKVDTALFPENFMPGQIGAAVSFNTGDNFPDTENIQ
                     IALLGVPENRNSQSSRDASFAADAIRPYFYTLQSHFPNVAIADLGNLKTGMSVDDTYA
                     AVAEVVNILLKDNIFPFILGGSQDLTYANYLSYEKRGRIINILSVDSRFDIGKSDETF
                     HNESWLNKIIFRDPNFLFNYSNLGYQTYLVEQEAIALMKKLFFDSTRLGQAKADMTET
                     EPLIRNADLLSFDMSAIRMSDCPAASFPSPNGFNGEDACQIMRYAGLSEKLSSAGIFE
                     LCPANDRDGMSAHLAAQMLWYFLDGFAGRKGDDPHMNKSALMKYIVSMHETQSDISFY
                     RSPLSDRWWMEVPCPNNLFSKFERHYMVPCSYRDYEEAMQENLPDRWWQTFQKFM"
     gene            67917..68918
                     /locus_tag="PKY63_00225"
     CDS             67917..68918
                     /locus_tag="PKY63_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPE99064.1"
                     /translation="MRKNVLFLFLVILASGAVNAQQDAQFTQNMFNHMAINPGYAGSH
                     DAICATALFRQQWMGFTETVHDNTNKVAPQTMLFSIHSNVDAIRGGLGLVVYKDKLGY
                     EDNIGVKFGYAYRMPLQGGTLGIGLMAGFLNKKMDFSQFNPQTAGDPLLASQAVESDM
                     VFDLSFGAYYNKPGVFYAGLSSSQLMQSQTDLASTVTTLGSPQLKRHYYLIGGYQYQI
                     PSNPQFELRPSLLIKSDFASTQFDINALMYYNSQFWGGLSYRTQDAIAILLGAQPFIN
                     SGNRSLETLGIGYSYDVTTSAMGGGGRSSGSHEILLNYCFKIVITIPPDSYRNVRFL"
     gene            68991..70391
                     /locus_tag="PKY63_00230"
     CDS             68991..70391
                     /locus_tag="PKY63_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPE99065.1"
                     /translation="MKSNLQSNMKYLTALVLLVLVITSCDTSDRGNLVGVQDREPWYQ
                     PDPYGMLFIPMGSYVMGQNDQDVPYSQLQTAKTVSVQSFYMDETEITNNEYRQFVYWV
                     RDSIARQLLGEQFEDYLIMTDEYGQDLEPPYLNWKTRLRYDDDDETVREILSQMYMPE
                     HEQFYRRKEFDARKLNFQYYWIDFKAAARKDFRNPLTDAQVKYEEDAASLRNRPQGYN
                     DRSVFIKSDIINVYPDTLCWVMDFTYSFNEPMTQNYFWHPAYDHYPVVGVNWKQARAF
                     SIWRTQYLHNYLRSIGQAIVNDFRLPTESEWEWAARGGLELSPYPWGGPYIRNSNGCF
                     LGNYKPLRGNYPDDGGFHTLIVGHYAPNDFGLYDMAGNVAEWSSNAFDESAYQFAHDL
                     NMDYSYEAKDTDPEVLKRKVIRGGSWKDIGFFMQVSSRWYEFQDTGKSYIGFRNVQTY
                     LGRMKGDGPNSSNVYR"
     gene            70443..71495
                     /gene="gldL"
                     /locus_tag="PKY63_00235"
     CDS             70443..71495
                     /gene="gldL"
                     /locus_tag="PKY63_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPE99066.1"
                     /translation="MGIKQIVESKGYKNFMSKLYGWGASLVILGALFKIMHYPGAGVM
                     LLIGMGTEALIFFASAFEPMHVEPDWSLVYPELWGIYHPEEAEKLGWEPQKSVATGKT
                     VTQELDKMLADAKIGPELIASLGEGMKNLSENANKLSGVADAASATDGFVTNLGAAAS
                     SVSGLKEVYDKTANSLSKTVGVNDEFVETVKAAANNANETAGSFKAVANAINNDVNAT
                     EEYVTSIKGATTAAHALAQKYTQSAESLTKSAEAIDFSAVDGKSYGDQIQRITKNLSA
                     LNAVYELQLQGANSQVEKANAMQESLGAFVDNVNQTMTALNSYKDQANVLSKNLAALN
                     TVYGNMLSAMNVNLNK"
     gene            71537..73180
                     /gene="gldM"
                     /locus_tag="PKY63_00240"
     CDS             71537..73180
                     /gene="gldM"
                     /locus_tag="PKY63_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HPE99067.1"
                     /translation="MGAKNCPETPRQRMISMMYLVLTAMLALNVSKDILQAFTVVNDS
                     IVETNRIFKAKVDGNYNMFKSAYALTPGKVKENYEKAQTVQKEADALVAFIQQVKWEI
                     ISKVEGKTPEEVKAMEEAAAKEGKSFLADIDGKDNYDVPHHYFFGDSEDGGSGTKVFE
                     LKDKIIAFKKKMTDLLGPKYAKSVNLGLEVEKDYPTVSGEGTANWQVTNFYHTILAAD
                     VVILNKTILEVRNAEADVVAQLYNMVDAEGFSFDKVEAKVIAKSNYVLTGSEYEAEIF
                     VAAYDSKQQPTILVGSGVDSTTLEVSGNVETIEGKDGVGQYKVGAGSIGEQKFGGVIE
                     VTSRNGVKTKYPFNASYFVAQPSATVSADKMNVFYIGVDNPVTISVPGVANEKVRASI
                     SNGSLSPTGGGHYNVRVTGGAEAVINVSADMDGSSRPMGSTKFRVKPIPTPIPKVANK
                     ISGNFTKAEILGSPYVLAVLENFDFDLRYNVVSFKFTYKNPAGDLIDIPGQGYLLNQQ
                     MKNLIQNSRRGDRFWVEDIIAAGPTGNKQIGSVTIRITQ"
     gene            73191..74033
                     /gene="gldN"
                     /locus_tag="PKY63_00245"
     CDS             73191..74033
                     /gene="gldN"
                     /locus_tag="PKY63_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPE99068.1"
                     /translation="MKTLFVSVVVMLGFTMASLAQVFDNPPDRVWDKEYYPDRVAVPY
                     PHLRKADVFFAKRIWRVIDLREKINHPLYYPIQPTNGRISLMSTILKGIEENSIRAYS
                     AIDDEFKTPMSPEEVKGLLVKIDTLKDRFDPFTGAQLPDTVITTPFDVASVTMFRLKE
                     DWVFDRQRSVLECRILGICPLREEYDIDGNLKGTRADFWIYFPEARNVLVKNEVFNRF
                     NDAQRLTYDDWFFKRLFGSYVIKETNVYDRYIVSYATNLDALLESERIENDTFVFEHD
                     LWEL"
     gene            complement(74085..75623)
                     /locus_tag="PKY63_00250"
     CDS             complement(74085..75623)
                     /locus_tag="PKY63_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPE99069.1"
                     /translation="MLVKTFCCAIQGIEAYTVSVEVDIQSGTKTFIAGLPDNAVKESI
                     ERIRSALVHNGFRYPGRRVVINLAPADMKKEGSAYDLAISIAVLAASHQIESERLSEI
                     MIVGELSLDGTIAPVKGVLSAAIEARKNNFKGIIVPAENAHEASVVDGLQVFPLHDIR
                     QVVQFLEGTTEMDPCPSGYSISEEKGSPYPVDFSDVKGQENVKRVMEIAAAGGHNAIM
                     IGPPGSGKTMLARRLPTILPPMSLDEALETTKIHSVSGKLPQGTGLLKNRPFRAPHHT
                     CSDVALIGGGTYPQPGEISLAHNGVLFLDELPEFKRSALEVMRQPLEDRIVTISRARF
                     TVDYPASVMLIAAMNPCPCGFYNHPEKECTCAPGAIRRYLSRISGPLLDRIDIHIEVT
                     PVPFSSLTDKKKSESSQAVRQRVIATRMIQQERYKEHSQISCNAQLTPALMKKYCQLK
                     PEGEQLIQKAMDMLKLSARAYDRILKVARTIADIEQSADIENHHLAEAIQYRSLDRET
                     WGMV"
     gene            75720..76142
                     /locus_tag="PKY63_00255"
     CDS             75720..76142
                     /locus_tag="PKY63_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99070.1"
                     /translation="MKRFFNIFLIALMSATLLVSCQPDEDDDDISNDPRSDYQGVWVC
                     SEIGGQSYTVNISIDTATQSQITLFNFHHQGFEEKINAIVSGTSLTINPQTMCMGTLT
                     VEGTATMQSNKTNMNFYYTVNNGVDMDTIQANYTKQSS"
     gene            complement(76567..76929)
                     /locus_tag="PKY63_00260"
     CDS             complement(76567..76929)
                     /locus_tag="PKY63_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPE99071.1"
                     /translation="MYKKLIFILFVLLLAVACSSKKETSEISPDSVFTQDSMKTLLID
                     FYLTEASIRQIERTGKDVSVHATHYYQLMLEKYHCDTSKITRSYRYWAHQPEKLKQIS
                     NQALDSLIILETMLEKDQ"
     gene            complement(77247..79352)
                     /gene="recG"
                     /locus_tag="PKY63_00265"
     CDS             complement(77247..79352)
                     /gene="recG"
                     /locus_tag="PKY63_00265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPE99072.1"
                     /translation="MSDSILNNKIEYLKGVGPARAEILNKELSVFEFQDLLNYFPFRY
                     IDRTRITPIATLSGDDNFVQISGFFDEFVKVGKTGHSRLEAIFHDDTAAIKIIWFQNF
                     SWVTDKYKTGTRYYIFGRVGLFSKQLYMAHPEILTPDEFKRLPFSGRFQPIYQTSEKL
                     KAKGIDSKAISMFVAAALNKTSGQMNETLPEKLLKSLQLPGINEAYQNIHIPQDENIL
                     DRSLKRFRFEEALLMQLRHKINADNRKKTSTGIIFKEVGKYFNAFYNQSLSFALTAAQ
                     KRVTREIRADLGSGYRMNRLLQGDVGSGKTVVAMMAALLAIDNGYQACLMAPTEILAQ
                     QHFATISKWITDLGLRVELLTGSTKNKGRKEILKSLADGSIHIVVGTHALIEDPVEFS
                     NLGLAIIDEQHRFGVMQRAKLHSKGSEMVHTLVMTATPIPRTLALTFYGDLDVSIIDE
                     LPPGRKEIITKFYYDDQRYYVIEMMRKQIKNGRQIYIVYPLIKESEKLDLVALENGYE
                     GIIRDFPMPEYEVGIMHGRMPQDEKDFVMKRFVRGLTNILVSTTVIEVGVDVPNASVI
                     IIENAERFGLSQLHQLRGRVGRGKFQSYCILIGGKNLSNDAKTRLKAMIETTDGFKIA
                     EADLKLRGPGDLMGTRQSGEFEFIHLDVVRDDHIITFARQTAEQICTDDPTLSKAENA
                     CLKTALKERFKGLSLSDIA"
     gene            79435..80475
                     /locus_tag="PKY63_00270"
     CDS             79435..80475
                     /locus_tag="PKY63_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99073.1"
                     /translation="MCIIKVASNVIVLMKYPGRLFLMWFFSVTMLHAFAQKEKLVVQT
                     PSTDSLMAASMAMYNGKTDMARYEANEKFIQLLKKDLREENAFDYVFDSIRILSVIYP
                     DDRKFKVFSWNLPLSNFKNENYGLMMVFSEKSHNYKLIELRDVSNDIINPEKEILKKG
                     LWYGAVYYQLIEKKFDGKKVYTIIGWHPTNGLYQQKIIDVITFDRNDDPVFGKLMFRG
                     KGYTGSKRIVFRYSDKVAMKLRFEEATYNIVKKKEKKKYNSRINKNNDRALRKPKKKT
                     IVKTKMEEMIVFDMLFPTRPELQGQYQYYYPLSETGNGFYFNDGRWVHRSLEPAEDTL
                     LVKPINNGLMPD"
     gene            complement(80488..81453)
                     /locus_tag="PKY63_00275"
     CDS             complement(80488..81453)
                     /locus_tag="PKY63_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99074.1"
                     /translation="MKSYTNDVEATEGLRTLYNQLKSEVAKVIVGQDEVVDKILISIL
                     CKGHALLIGVPGLAKTLMITTIAKTLGLSFSRIQFTPDLMPSDIIGTEILGEDRQFRY
                     IKGPVFAHIVLADEINRTPPKTQSALLEAMQEREVSVAGQTHQLPDPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFSIWLDYPSMDEEIEVVKRTTVEKMPEANVVTTQEELVMFQN
                     LVRRVPVNDSVLKYAVNLTAKTRPGTASVSPIADQYIAWGAGPRSSQYLILGAKCHAL
                     LHGKYSPDIEDVQAVSSNVLQHRIVKNYKAEAEGLNSREIIGKIL"
     gene            complement(81456..82796)
                     /locus_tag="PKY63_00280"
     CDS             complement(81456..82796)
                     /locus_tag="PKY63_00280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE99075.1"
                     /translation="MKQLILPLLFALMLPTAAMAQPSGETIDKIVAIVGEKIIMLSDV
                     ETQYWQLVMQGEKQTDELRCSILEELLLQKMLLTQAEIDSLVVTDDQVQAEIDRRLRY
                     FIQQLGSPEALEKYYNKSIPEIKDEFQDLIKDQLMVQQMQGKITENVSISPSEVKKFF
                     NGLSADSIPFVESELEIGQIVVIPDPSSADKQFIQQKISDLRARILKGESFEALARLY
                     SEDPGSAKNGGELGMFSRGQMYPEFEGAAFSLKNPGDVSEVIETPAGFHVLQLIDRKG
                     DYINVRHILLMVKASPTDLYAGKLKLDTLYNQIVAGTISFAEASDKYNPEEYRSNGGL
                     ILNPYSGSTKFKPSEVDASVFFTVDKLEAGSISKPVLFQSDDGKEGYRILFLKSRSIP
                     HKATLSEDYNLITQFALDAKKRQALEKWIASKKSSTYIKIIDDFQSCLFKYKWE"
     gene            complement(82801..83655)
                     /locus_tag="PKY63_00285"
     CDS             complement(82801..83655)
                     /locus_tag="PKY63_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99076.1"
                     /translation="MIIRCQILSVVGLSLLFMFACNRKQSSDTLLAEVDHEQLYISDI
                     YFPENISAEDSVSFLKNMVQSWIVEQLMYIEANTKLCEQEMDEIEKQMERAKKLMIIS
                     ALEEKLVSDSLKIMVSDEEINKYYRENQDEFLLKENIVKVQYLKLQKEATDIAKIKTL
                     LKSDNAVEKAELMSIAREKALNYFLEDNVWLYFNDILKEIPIKAENQAEFLKTNTFYE
                     LSNDSVVYLMRFKGYMLTESPAPLVLVRDRIESVLIARKKNILLDDYRNKLYENALKE
                     KKVTLHID"
     gene            complement(83655..85637)
                     /locus_tag="PKY63_00290"
     CDS             complement(83655..85637)
                     /locus_tag="PKY63_00290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE99077.1"
                     /translation="MKSVLFSAFLFFLAFSGIVAQNDPVLLTIENEKVTKSEFLAVYN
                     KNNVKEEPLNAENVKEYLELFINYKLKVKEAESLKLDTSKSFINELAGYRRQLAQPYL
                     SNREVTDQLLLEAYDRMKWDIRASHILIKVSPNASPADTLKAYNRIMDIRKKLVAGGK
                     FDEIARKYSEDESAKDRPGRDGQSMMKGNGGDLGYFSVLNLYYEFETAAYNLKTGEIS
                     NPVRTPVGYHLILVNDRKPALGKVQAGHILIKDVPGKEDSIKARIDEAYKQILDGVDF
                     ETVVKNYSDDKGTVAKGGILPWFGSFRMVPPFVLPLYDMKPGEISKPVQTIYGWHIIK
                     LVDKKPIGTYDESKSTLKTQISRDKRAFIARDKLVDKLKTEYKFKADENVLKELVPLI
                     NDSIYMASWHVPAAPSLSKPVCTIAGNPYLLGDFADYLMHKQSQIKEGDDIYTFVKTI
                     FNQYSEEMIIEHENTKLEEKYPEFKALMKEYRDGILLFDLMDKNVWSKAVKDTSGLKK
                     FYETINQNYLYGPRAEAAIYTCKGETELKALQKLLKKAPKKGYTPEKISSILNKDSIP
                     AITYQIVKVEKGVNPLVDQTPWVTNQNSVIRQNNEIKLVSILNLLEPQPKPLQEVKGL
                     VTAEYQNYLEQQWVSELRNKYTWKVDEKVLDSIYQK"
     gene            complement(85666..87135)
                     /gene="guaB"
                     /locus_tag="PKY63_00295"
     CDS             complement(85666..87135)
                     /gene="guaB"
                     /locus_tag="PKY63_00295"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPE99078.1"
                     /translation="MSPKDQKFLPEGLTYDDVLLVPAYSEVLPRETSLVSRFTRNIQI
                     NVPFVSAAMDTVTESAMAIAMAQEGGIGVIHKNMTIEQQAIEVTKVKRAENGMIINPI
                     TITENKTVADALALMKEHKIGGIPVIGSDQKLVGIVTNRDLRFEKKLSKLIKSVMTYE
                     NLVTVREFTNFEKAEEILQKHKIEKLPVVDKNYRLIGLITYKDIIKIKARPNACKDKL
                     GRLRVAAAAGIAANTPERIDALVAAGVDAIVIDTAHGHSRGVLNMVKTIRKKFTGIDI
                     VVGNIATAEAALALAKLDIDAVKVGIGPGSICTTRIIAGVGVPQLTAVHEVAKALKKT
                     GIPVIADGGIRYTGDITKAIAAGAHSVMAGSLFAGVEESPGETIIFEGRKFKTYRGMG
                     SIEAMQHGSKDRYFQDTEDDIKKLVPEGIVGRLPYKGTLSEVMHQLIGGLRAGMGYCG
                     SPDIAALQNAQFVRITNAGIIESHPHDVTISQEAPNYSR"
     gene            complement(87241..88662)
                     /locus_tag="PKY63_00300"
     CDS             complement(87241..88662)
                     /locus_tag="PKY63_00300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPE99079.1"
                     /translation="MKLNRTKQTARYVVCDLLGAAAAWAALFSFRKYQGGMQFEELWD
                     VVSADPNFFLGIFIIPLSWLLFYFITGFYRNIFRRSRLRELGQTAMQTLMGVTVIFFV
                     FLLDDRVNSFVNYYESYLFLLGTHFIATYIGRFIITSATARKIHNGIWGFRTLIVGNN
                     GNAVRIYEDITNQAQSAGNIFTGYIPVLENSNDKLSKYLPRLGSVSELEQIIEEEQIE
                     EVIVAVEPSEHKVITDILSILDSTSVDIKIIPDYKDILAGNVKMNSIFHVPLIYVSRE
                     LMPHWQRVIKRGFDIVVSVLVMTVLSPLYLITAAIVKFGSKGPVIYSQERIGLHGKPF
                     VMHKFRSMYLNAEEDGPALSNKADSRITPFGRFMRKYRLDELPQFYNVLIGNMSLVGP
                     RPERKFFIDKIVQQAPYYKLLHKIKPGITSWGQVKYGYAENVDQMIERLKYDILYIEN
                     ISLAMDFKILIYTVLIVFQGRGK"
     gene            complement(88659..89513)
                     /locus_tag="PKY63_00305"
     CDS             complement(88659..89513)
                     /locus_tag="PKY63_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99080.1"
                     /translation="MRLFAIFFLTAVIMTSCNWINPSEETPSFIKVESISFSTESTEG
                     TENQNFTDAWVYINGEKTGAFEMPLTFPVLKEGPVTIQIYPGIILNGISNTRAIYPFV
                     KPWEATINLVKDSVSIIQPSSTYYDNLEFRVVEGFEDAGMTINSTTLSDTIMLRTSEP
                     SEVFEGSFSGMLAIDTQRDTIDVRSNASYVLPQTGDYIFLELNFKTQAPLVVGVIANK
                     GGYSVYHPLIILNETNTWKKVYINLSPVVSREYDADSFFFFFRMELPEGMNEAKAFID
                     NIKLIHAE"
     gene            complement(89574..90566)
                     /locus_tag="PKY63_00310"
     CDS             complement(89574..90566)
                     /locus_tag="PKY63_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPE99081.1"
                     /translation="MLRIGVLGAGHLGKIHIKCLKELENYELLGFHDIDAERAKEVEK
                     EFGIRHFENREELIDAVDAVDIVTPTISHYEIAALALKKFKHVFIEKPIVTTLNEASS
                     LVNLSREANVKIQVGHVERFNPAFLAAQPYINKPVFIETHRLAQFNPRGTDVPVVLDL
                     MIHDIDIVLSVVKSNVRKVSASGVPVISNTPDIANARIEFDNGTVANLTASRISMKNM
                     RKSRFFQKDAYISVDFLEKKTEIIRMSDVIDESKLPPFAMVVDPGNGAIKKQIHIENP
                     PIETINAIKMELTKFHDSIVNNEPTPVGILDGYTALEVAYKIIEEINETLYFFS"
     gene            90669..91340
                     /locus_tag="PKY63_00315"
     CDS             90669..91340
                     /locus_tag="PKY63_00315"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPE99082.1"
                     /translation="MITYKGRVLEDNYRHQGLRKRLVAELEQKGINDPRVLAAIGKIP
                     RHFFMDSSFVEFAYVDKAFPIGEDQTISQPYTVAFQSQLLQIAPRMKVMEIGTGSGYQ
                     AIVLMELGAKVFSIERVKALYDRTREFLPAIGYLPKLFYGDGYKGLPTYAPFDRIIVT
                     AAAPVIPDALLQQLKPGGILVIPVGDSAGVQTMKTITRVDEKTFTEKDHGLFRFVPMV
                     ENKRK"
BASE COUNT        24774 a        18065 c        20520 g        28096 t
ORIGIN      
        1 tctccccctc cacaatccca atctcctcct cactcaaacc atacaactca tacaccaacc
       61 ggtcaatctc cgattcgagg gcggtggtgt cggcggctgc attgaaactt tttgcatcaa
      121 ttatttgatc tgcaattgta atgaactcgc ttctgttttc agactcaaca ataggaaaat
      181 ctttaaaata aagatagctt ggttcataaa aatcacctct caatttcggc aataaattct
      241 gaaatagaaa ataagttacc cggctattta ataatgcgag taaatattta tcagcttccg
      301 gaatgatgaa gcacttttga tttgtatatt gcttggaagt atcatacaca aattccggtt
      361 gtttgcatat gttaggataa atgatttttc ctttctcaaa ctcttcataa taatcgcatg
      421 cccttaattc ccaccagaaa tcgcctttat cggtgcgtac tgttgcttta cttttgaaag
      481 ggagcaagtg attggcaact gctgggtatt tttttgaaaa ccattcccat gcagcatcat
      541 attccatatt tccatatcga ggaggtggtt catttgcttt aaatgaatcg atttgcagaa
      601 aactctttat tgtaaatcct tttggaataa gaattaaata tttgtcgctt tttggttgct
      661 gataccgctt aatatcgcgg ccggctaaaa acggtttaat aatttcggca ctagacggat
      721 tttcagcaat catgcgactt ttagtttctt cgtcaattac gaaagcttca ttcaatccgg
      781 ttaatacacc tctgaaaatt ttcccgttta catattcacc aagcggaatt cctttgcttt
      841 taattttttc aagaagtttt tgcttgctgt cgtcaaccag ggtccatcct gaatcggaaa
      901 gcatttcaga atttactttg aacgcaatgg tttgaatgta gctctccagt ctatcggtaa
      961 aatcgagcgt ttgaatattg gaagaaataa aagtgttttg agttggcttt tgtttgataa
     1021 tgttccaaag gcaaggatag gtggttgctt cttcaaacac tggcaggtcg ccaaagtcgt
     1081 gaataaaacc aattgtgtat tgctgaaccc attgtctcag agcattgccg tatccagctc
     1141 tcatccactt gttgggcaga atgtaaataa aacttccatt ttgttttgta atctgtaaac
     1201 cacgctcaac aaagtaaaca tataaatctg ctgtgcctgc aaaagtttca aagtggctct
     1261 gcaaatatgg tttaataaca gaaaaatcct cctgtcggat atatggtgga tttccaatta
     1321 ctacatcaaa tccaataaaa tctccattgt cattaagcac ttctggaaat tcaaaacgcc
     1381 attcaaaagc attgtcgtaa atcttgttgt tttttatttc ttcaaggtct ttttcaatcg
     1441 aggtgatttc gccggtgagc ttttcaactt ttttattcca ggcggctttt tctttcgcgc
     1501 tgcggtcgaa catggtcact tctgttgtaa gcgaaaccag ctcgcctttc agcaaattca
     1561 gttgtttcag acgtttgtcg ttaatggcaa tttcgctttc aaaatcgctt ttgatctgat
     1621 tgatcagctt ctccatttcg cgcttggtct ctttgcttga ggcttcgcga tatgattgaa
     1681 tggctacttt gtaagcatca atagtccact ggcttttttt cagagcctgc ttaatatctg
     1741 catccagtgc atagcggctg atgagcgagt taccgcattt gatgttgata tctatgtttg
     1801 gcaatgtctc taattctgtc aaccctgcga atgacccttc gactccgctc agggtgacat
     1861 tcttcgggtt gccagagctt ttgtagtagg cgtttttaag caattcgatc caaaggcgca
     1921 ggcggcatat ttttacagag tttggattga tgtcaacgcc aaacaaacaa ttttcaatca
     1981 gcgtttgctt ttcgtgaaac aaggcttcct gcacgcgctg gctttctttg ttgccgggtt
     2041 tatattcaaa tatttcaccg tcttcgtcgg tcagaatgag ttcgtcgttc tgaatttcga
     2101 cattgtattc tttcagccga cgcccttcgc ggtcgcacag aatgcgtaat tcgctcttga
     2161 tggaaatcag ttcattcaat gccgaaacca gaaaatgtcc ggagccgact gccggatcac
     2221 agattttaat gctgtttaca atccggtttg cttcggcact gtcttcaatt ttgttgtaga
     2281 gctcatccag cgtattgcag ctccagcctt tggtttcgtt gaacttttgc accaccgccc
     2341 tgcgaatggt ttcgtggcac atgtacatgg ttatgaatcc gggcgtaaaa aacgatccat
     2401 cgcgatagcc gtttatcttt tcgaagatga gaccaagaac agatgcgtta atcaggtttt
     2461 tgttgtcttc ctgaatttcg cccgatccat cgctactgaa atcataagcg tcgagaaatt
     2521 caaatagata ttcaagtgag cttacatcct ttgctttgga atatttgtcg ctgcgcctca
     2581 gtactgagtt tgcaatcaga ggcaacattt cttcgtcgct cagattgctg ataaaaatcg
     2641 attgctgctc aatttcggat tgttcaaaca aggaactatt caaataagga accttcgcaa
     2701 aacgcgcttt aactcttgcg ttgcgctcat cgggcaattt agcgagcaca ctgaaaaata
     2761 actgattcag ttcatcgaag ctgctgattt tatgaatact cataaacctg aattcagaat
     2821 tgcctttgtg ataagacaat aactgcgctt cgagcagttt cagaaacaat atccggttga
     2881 tccacgtaat gcagagctca agcccgagat tgaaataccg ctcttcgctg gtttctccaa
     2941 aacgtgatgg atttggaata acactgaatt tatcaagatt atcaatttgc gaaatggcgt
     3001 tttcgagcag tgacccggca ttacgctcct gttccttttt gcgggtaatg atttttttgc
     3061 ttccatcttt tatttcggtc aatccgatga tgtgaagcaa ttcgttataa aaagccttgt
     3121 tcagactgtt actgtcgttc gagaattgtt ttttgagcaa atgctccggc gaaaatattt
     3181 tctgtagtgc aatcagttta ttgtcgtccg aaatttcgga gtttcgcagc ggcttttcat
     3241 actccgaaat atcgaaatgt gtgaaaacga tattttcttt tacggcttca atagcgggtt
     3301 gtgctacttc cttgtagaaa aattcagttt tgtttcctcc cagacgtcca gcttcaaagt
     3361 cattgaaaag ttgtaccagt tttttattct gggcaaacaa cttttcgaaa agttgcgcgt
     3421 cgaaaatata ccattcaaaa atatctgttg caataagata cttcagttgc agattgttta
     3481 gtgtaatgcg ttctctcaga taatacaaca ccagctcctg catagccttg gtgtttacat
     3541 tgcctggccg aagcatctct gttttatttc cgggcttttt ggtttcaaca ataacgccga
     3601 cgggactttt ggcatctttc ccattgtgta tcaccagatc gttgcgccct ttggtgttta
     3661 tgaagtgctt gtctgaataa taggttttgt acagaaaagt gctcaaaagg ttcttgtgaa
     3721 actcttcaga ttccgagtca ttacagtcat cgagcagctg aaccagattg tttttgaaca
     3781 gggtaatatc gctgcgaagc ggtttcattt tcagaaacgc cttattcaaa gctttgcggg
     3841 gagaaagtat atttttatcg gacatgattt gatgttaatt caatcaatat ttcttcccgc
     3901 aaatatactt tttttcagga taaactgaaa ggaaatttaa acataatgat tgaatgcctg
     3961 atttatcatt taagcaaata cgcaggtttt cttgaggaat aaaggaatac tgaaatgaga
     4021 aaattaatta cttgttcaaa acccgcttaa tgtctgcttt gcaggtgtgg cttatgacgc
     4081 tggagtaata agaaatatgt ttcagaaatc aatcatcatc atattctttc cttgtaatcg
     4141 gaaagctgcc gtcgaggatt ggtttttttg attctttcac aatgaatttc atggtgatat
     4201 tgctgtagcc gcgtttgctg tcggcaatga cttcaaaggg tgtacccttg ctatacaatg
     4261 tgtcgttcag ggtcagtgtg tctttggaaa tcagcactgc cattgtcttt gtgaagtaac
     4321 ggccggtagt gttgaaatat atcgtttctt ttccttttgg ggcaattgta agggtcttgt
     4381 aaagttcagc agtcctttcg gtgcctttaa tcactttagt ggtcatcacc cggtccgatt
     4441 tattgatgat tgtaagactt gcctgttgcg aaaatccgca ataagacaaa agaagcaatg
     4501 aaaggaataa taatgacaga ggtttcatta gtgccttatg ttaagtataa cgttttgaca
     4561 aaggtattct ttttttaata aaatcagatt aaaaagtcat cactctttca aaaatccttt
     4621 catccctgtg ttgcggccac tgccgatgcg gaggaaatac aatccaggag taagcattga
     4681 tacatcgatt gttgctgcat ttccgctgat gatttgtccc gaaatttttt gtccgcgagt
     4741 attaataagc gttacgttaa tttcgttttc gatgtaaatt aaattcagct gaattatgtt
     4801 ttgtgcagga ttcggatata tctcaaacga tggtatggtc tgtatttccg ataggccgat
     4861 ggtttggtcg cagacttgtg agctgttggg aaacagtact gtgtctgcca ttgagaaaca
     4921 atacatttgg tctgaacttt cgaaaggaac gtagagctcg cccagtaatc caaaagtgct
     4981 ccctacgcct tcgatgatgg aaacgaaggc cgaatcgtgg attgagcccg atatttttac
     5041 tccgagattc cattttttgt gataggtgtt tccgagtaaa acagaatctt cggacagcac
     5101 catcagtgta tcggttgacg agatgtacgg attgttgatg gtgggcggat acacctgacc
     5161 aacactcagg ttgaagtcga acaacagatg ttcattgtta tcccattcgg aatattgcca
     5221 aacggaattt gttgcagaat caattcttat aatggcatac agttcttcgg gtatgttgaa
     5281 atcgcagcta aaaatgcagt atggcgacgg gctggtgagt attgaatgac agaccgatcg
     5341 cttgtaaagt ctgtgataat tggttgcgtt gatgactgta tcggatttgt aataaaccgg
     5401 gagcatacaa cttgtgtaat agccgcatcc gggtgtacat tcgtgttgaa acagctgtcc
     5461 gtgttccagc atccagaacg ggtcttcggc cggagtagga aagtgatgtt gtgcacttgc
     5521 agtagtgact atgcagagaa tgatgaatgt gtacaggagc gttttcatgg acgaattgtt
     5581 tgtatcagta aagatatgtt ttttcaatga aagttgtatg gattgtggtg tttgttttta
     5641 ctgaactttt gcagggaatg taatgacaaa ttcagtccct tttgaagagc tgtttacatt
     5701 gagagatccg ttgatttgtt cggtgagaat acataccagt tgcagcccca gactcttggt
     5761 gttgtaacag tcaaaatctt caggcaggcc tttcccattg tctttgattg agtagataag
     5821 atttttttca tctttttttg taagactgac ttcaatgagt cctttggaac caggctcgaa
     5881 tgcgtacttg aacgaatttg ttataatttc attggtaata aggccaatgg caataatggc
     5941 atcaatattt actttatatg atttaccgct gattttgcat tgtatgtttt cgtcgggcga
     6001 tttgtaaatg gatgtaagaa actcaaaaag atttttgatg taatcaatca ggtcaatgtt
     6061 tgaaaaatcg gtcgtttcgt atagtttttc gtgtaccaga gccattgttt taatgcgtgc
     6121 ctgactatcg cgcagaatgc gtcttatacc ctcatctgtt actgttgaaa gttgcagatt
     6181 gataatgctg acaatcacct gcatgttgtt ttttacacgg tgatgaattt cctgcagcaa
     6241 tacttctttt tcctgtaact gatgcttgat ttgttcttct gctttcaggc gttcattgat
     6301 ttcaacagta agactctcat ttgttttgac cagctcgttt gttctttgtt ttactttttc
     6361 ttcgagggtt gaattgagtt gtatgatttc tgcttctatg gccttttgtt tgctgatgtc
     6421 catgatcgca gcactgaatc cgaacaattc gccacttgtg tcaaacaaag gagtataggc
     6481 cccataaata tgggtgattt tgccattttc aaattccacc tgatctgaaa cttcaacagc
     6541 tttttgagtt ttgcggcatt tgtcgagaaa tgctttgtga cgtttgtata aatcgggctt
     6601 gaaaaattcg ttgaagttgt ggttttcgat attgttcagc ggcagtttga aagttttgga
     6661 gtaatattga ttggcgataa agtattttcc atccagatcg actgcagcaa gccacatggg
     6721 tagcgaatca atcatcgatc gcagcatggt acggttgcgc tgaagttcaa tttctataat
     6781 ttttcttcca gtaatatcct gcatggttcc tgccgatttc tgcattttct ccccttttga
     6841 tacatggggg atgcaattga caaaaacata ttttactctt ccgtcgggca tgagcagccg
     6901 gtgttcaaac tgaattccgt ctttgttgtt tgccgatttg tcaaattctt ttttcgcttt
     6961 tattctgtct tcaggatgga tgcgttcgat aaacgacttg aaaagatctt tttccgtgtt
     7021 tttattaatt tcaaatatta tatacatttc atcggaccac ttaatctcac tattggtgaa
     7081 acaatattcc caattgccca gcttggccac atgttgtgct tctttcagtt gcttttcgct
     7141 ttgcagaacc gtctgctcaa ttcgtttgcg ctcagtgatg tcatttatta ctgcaattcg
     7201 tgcatttctg ttgttgtatt taatgtcatt cgatgtgacc agaatatcaa tcagcgagcc
     7261 gtcggctttc aggtgtttcc attctccggt ataggagtgg ccttgaattt ttcgagccag
     7321 gtcaacaatc ccatttcgca gttcaggcgg ataaaaatcc tgcagatgca tgtttgaaat
     7381 atctttgacc gaatatccat aatgtttcag aaaagattcg tttactgaaa taacctcaag
     7441 acttcctctt tcataaatca gcattgggag cggattttgt tcaaataaaa gacgataact
     7501 ggcttctgac tcacgaagct tatgttctgt ggtttttatg tcggttatat cgagtaatga
     7561 agctacactt ctttttgtcc cgggaataat atcaacggta agaaggatgt atttctgcac
     7621 tccgtttttt ccaacaaatc tgaattcgta tgatttcaat gcagactctt gtctgatgcg
     7681 tcgcagcata tgttgttctt ccattcgcac cagatcttcc cagacaacaa attctttcca
     7741 ggttttgcgg ttttcaattt catcttttga atatccggac aattcttcaa atttggaatt
     7801 ggccagcgaa atggtcgtgt cttcttctat aataactgct gcagtaccag tattctcaaa
     7861 aatggctctg tagcgcagtt ccgattcttt caactgcagt tcatttttct tggtattggt
     7921 tatatcatga aagaaaactg aaattccgtt ttcagtcgga tagatatgat tttcaaacca
     7981 tcggtcgtac ggtggataat attcttccaa gctgatgggg atccgctctg tcattacttt
     8041 ttcataattc agctgaaagg gttgtccaat gccttctgga aattcagtcc agatatgttt
     8101 gccaattata tctgcagggt ttctttcaaa aatttcaccg gcttttttat tcatataagt
     8161 gtaacaccag ttgaaatcga ttgacacaaa tgcatctgac atggattcga gaacatcgac
     8221 catgtgatcg tgttcctgtt taagtttgtc aatggccagt ttgctttcag taatattttt
     8281 cgaagtcatg gctatgcttt ttacgctgcc atcaggttcc agtaccggag aaatgtgtat
     8341 ttcaaatcat ttattattgt atgaatcgat aaagtttaat ggttttttgg tttgtatgga
     8401 ttctcttact ttttcgaggc gtgagtcaaa gatgtgtttg ggcaaaattt ccatggccga
     8461 atgtccaatc agttcatgcg gcttttttcc ccagcgcgat aaaaacgctt tattgccgtc
     8521 gataaaacat ccatcataat caacaatggc aagcgcatcg ggcgaagagt cgagaatggc
     8581 tctgaattgt gctgtggtca gatttttttc ggcctcgatt gtttttttgt gggtgatgtt
     8641 tcgtacaacg gctatataat tgggctttcc gtttatttcg gcataagtta atgccacttc
     8701 ggcccagaat gttgttccat ctactctttt tgccagccat tcaaaaacgc atcgccctgt
     8761 ttgttttgca gttttgttgt tttcaataat aagagcttca tcttcggccg atgaaatgac
     8821 actcagatca gccggattta gttcttttat ttcctcgggg gaacacccgt aaagctcgca
     8881 tgcacattga ttggcataga catatttacc tgaatcaaca tcaagaacca taatgccgtc
     8941 ataaattgaa tcaagcatcc tttgaattga ttcggccgag aacatgtttg acatagcgat
     9001 aatttattgg tggcaacaaa aaacatcctt ctttttttca gcaacaacgt ttttttgttg
     9061 ctgaggaatt attacacagc tgctgaaaag cttaattaat ttaacgaaga taatatcaaa
     9121 acttaaaaaa tgcaaataac aaattaaatt tcctgatgcc gccttttttc ttcaaactga
     9181 ttcaaactga cctttttctg accgatatag gcatatttga aaatatctta tacatttgtg
     9241 aggaataaaa ccaacccttg agccgttttt ggaaatatct gttttttgca gtttctgttt
     9301 tgataatctg caatgtgcga gcacaaagtg ccggtgaagt tacttttgac agttattcgt
     9361 ctgaaattta cagctatcga aaaggattgt cgcaaaacac ggtgcaatgc attatgcagg
     9421 attcacgtgg ttttgtctgg attggcacct gggatggatt gaaccgtttc gacgggtata
     9481 aatttcagat ttttcgcccg gattacacaa atcccaaagg taaattaagt tgcgaaagca
     9541 tcaatgccct ggctgaggat aaaaatggta acctgtggat tggtactgat aaagggctta
     9601 atctataccg ttataaagcc gatacttttc agacttttta ttatagcttc tataattcaa
     9661 acagtccttc atcggacagt attacctcgt tggtcataga taaaagcgga atgctttgga
     9721 ttggtggcaa taaaggtctt tgcagttatg atattgaaaa taaaaagttc acacgttact
     9781 attataatag ccgcaaagat gcttccattc cttccgacct gatatatgca cttcatgtcg
     9841 atgatgaaaa ccggctctgg attggcacca acaaaggtat ggcgttgatg gatattcgtt
     9901 cgaaaaaaat tttcagactg gatcagctga cttgcaaaaa tggtcaaaag ccccttgtga
     9961 cttcttttgc cgaagctggt aatgttatct gggttggtac ttattgtggc gtttatgttt
    10021 tcgataagcg caaactgaat gaatacccaa acaattgttt taaggatgcc ggattgtccg
    10081 cagagctgac ttcgcagccc ataagccgtt tgctgtgcga tacccgcggg cgtttttgga
    10141 ttgctactcc tgataacggc gtgtatgttt attatcccga atcttgcgaa cttaagcata
    10201 tttacagcga tttcaacagc cgatatggat taagcaataa ttcaatgctc agtctggctg
    10261 tttcgcaggg gggcgaagtc tggatcggta cgtggcatgg actaaatatg tattcggatt
    10321 attcttataa attgccgcat tttcgcatta ttggttctga atttgattac agcaacaacc
    10381 tgatttggtc gtttcagcag ttgcaaagcg ggaatctgtt gataggaacc catggtggac
    10441 tgattgagtt cgatgttgca acacgaaatt tctctttttt ttctgagcag gagtttggaa
    10501 aaatcccggt cagggcattg accattcttc ctgatggtaa tatctgggtt ggaacatata
    10561 tgaatggtct ggttctgatg aatcagcagg gtcatgtatt gaaaagattt tcggaggata
    10621 gtccggataa aatagctggt aatattatct gggaaattgt gcccgataat aagaacaata
    10681 tttgggttgc agcttatggt ggactgaccc gaatcaattt atcaaccttt gagacccgaa
    10741 tgtggaagta tccgggttat gaaagtaagg gatatatatc gagtagctcg gttcatcatg
    10801 ttatggttga taaatctgga atcgtatgga tttcaaccat caatgggctg aaccggcttg
    10861 acccggccaa ggataagatt tacgtatttc atcacgatca gggtaatcct gtaagtattt
    10921 cgaacgaccg gatattatcc acttttgaag actcgaaagg agtaatgtgg gttggcactt
    10981 ttggtggggg attgtgtcgg tatgaccgta aactgggcgt tttcagacgt ttcagccgtg
    11041 ccgaaggttt ccccgataat gtcatttatg atataattga agacaggcag gggtttttat
    11101 ggctaaccag caatgttggt gtgattcgtt ttaatccgcg tgatttttcc aatgtaaaat
    11161 atgacgtgaa tgacggagtt caaagtcatg agtttaatct tggtgcctcg tatatttctc
    11221 ctgaagggat gattgccatt ggagggatga atgggtttaa ttatttcaat acacgcgaga
    11281 ttaaagtgaa tcgccagatt ccaaaaattg taattacaga attcaatgta tttgggaaat
    11341 catatggaag ttatatcgaa gatggtgata ccattgttct tgagccgaat aataactttt
    11401 tttccatttc tttttcggcg cttgattata ccaatccttc aaaaaataaa tatgcctata
    11461 ttctgaagaa ttacgacaaa gattggattc agactgatgc tgcgcggcgt tcggctgaat
    11521 acaccgatgt gaagcccgga acatatcagt ttatggtaaa ggggtctaac agcgacggaa
    11581 tatggaatga gcagggatta tcctttactg ttattgtgaa accgccgttt tataaaacct
    11641 ggtatttcag gtttttactc attgctctgg cattgttcat tacctggttg tttatcagag
    11701 gtcgtgtaag aaaagtcagg cagcaaaatg aagttgaagt acgcatgctt aaaatagaaa
    11761 aagaaatgtt cgatctggaa cagaaagctt tgcgtttgca gatgaatcct cattttattt
    11821 tcaattcgct caattcaatc cagagcttca ttatttccaa tgattcagaa aatgctataa
    11881 actatctggc caaattcagt cagttgatgc ggcttatttt aataaccagc agagaggctt
    11941 atattccgct ttcgcaggaa atatcgttgt tgaagcacta tctcgacctt gaaaatatgc
    12001 gcttcaataa tcgttttcaa tacgaaatcc gtgtggaatc tggcatggat acagagtaca
    12061 tgggtattcc gcctatgatt attcagccct acattgagaa tgccatcatt catgggttca
    12121 tgaataaaaa aaccggcgaa tgccgtttgc tggtgcaaat gtgggaggaa gaagattata
    12181 tcctctgtgt aattgaagat aatggaatcg ggcgtgaaaa agctatggaa atcaagcaga
    12241 aaagcggtct gatacaaaag tcgcagggca ttataataac aaaagaacgt cttgacatct
    12301 taaacagaca gcttaaaaat aaaatttcag ttgaaatcac tgatttgaag gatgatgccg
    12361 gaaatgccac cgggacaaga gtgcggctgg tgattccatt cattgatctt taaacatttt
    12421 tgttgggaaa attcttgtat ttttgaacca aattatagag ccatgatacg cgcaataatt
    12481 atagatgatg aagatgcgtc gagaataaca cttaaagctt tgcttggtcg ttatgtgccc
    12541 gatgttcaga taattgcaga agctgtagat gttgaatccg gtctggcaac cgtaaaacag
    12601 tttactccgg atctggtatt tctggatatt caaatgcccg acgggagtgg atttcgtttg
    12661 ctggaaatgt tgccggaaat tgactttgat gtgattttta ccacagcatt tgaccaatat
    12721 gcaatcaagg caatcaaatt cagtgcactt gattatttgc ttaagcccat tgtgtctgat
    12781 gacctaaaaa aagcagtgga aaaacaccgg caaaaaatat cggcacctga cagaaatatc
    12841 agttacagga ctttgcttga gaatctgaat cccgagccac gtaaaatagt gcttcatact
    12901 tttgagggca tgcatgtggt cgaaacagcc gatatcatcc gatgtcagag tgatgattgt
    12961 tataccaatt tctatttaaa cggaggcaaa agaattattg tgtccaaaac ccttaaggaa
    13021 attgaagaac aactgaccgg taaggatttt atccgaccgc acaaatcgca tctgatcaat
    13081 ctccagtaca tcaaaaccgt tgtccgaaac gatggcggtt atatagtcat gaaagacggc
    13141 acagagatcc cgatttcacg acgaaagaaa gaaatggtca tggacattat caacaaactt
    13201 tgaaccgata acttttcttc cgttgccgga ttctgattcg tggcaaatgc tgatatgatg
    13261 aagatttact tttaaactgc aaatttcgaa ccaaccaaca attccgttat ggaacaattt
    13321 ataggcctga ttattttctt tgttgcatgg attattattt ctgcaatgaa aaaaactgct
    13381 aaaaaacagc aggaagcagc taagcaaatg aacaagaatg ctcagatgca gaatgcccag
    13441 gcagccaaac ctgcatcaac agccgatgaa atcaacaaac tgattgaaat gtttaccggc
    13501 gagtatgtcg cgcctaaacc agcatcagta acaacccccg ttgaacaatc gtattccgac
    13561 agcattgaaa ccattgaggc aatcaacgag gtcggtggca ttgaaacggc tgcaccatct
    13621 taccggtcgg tggatgaata tgtaccgtac tcttcttcaa tcgatgattt ttcttccgaa
    13681 gaaactcttc agtcgtcttt tgccggccag actgaaaata aaaatgtttt taaccggagc
    13741 aaaaatctgc ctaatccttt tttaactgat tttgacctta aaaaagccgt tgtttactcg
    13801 gtgattttgg aacccaaata tttctgacgt tgaaaaatct tttcgacaat ctgaaaaaaa
    13861 atcagcttaa aaaagtaaat ctttataact ttacccgctc aaatttaaag ctttcaaaaa
    13921 aaataaacca acccatatgt ttcgaaaact actctctgcc ttaggattga cattgtttgt
    13981 gagctcaatg gtgcttgcac agtcatccgg aacgttgaaa ggaaaattgg tggacaagga
    14041 aaccaaagaa cccatcccgt ttgcaaacat tatcgccgaa accggtggta agcaaatggg
    14101 cggatctacc actgatttcg acggaaatta caccattaaa ccgattcccc cgggaaccta
    14161 tgacgtgaaa gccacctatg tgggctacaa accgctgatg gttcagggtg ttgttgtaaa
    14221 agacggaatt atcaccttcc tcgacattga aatggagtca acagctgtta cgctgactac
    14281 tttcgaggta gttgattata aagttcctct cattgagaag gacggtggct cttccggtgg
    14341 aacggtcact tccgaagaaa ttgagaaaat ggccggccgc gatgcaactt cggttgctgt
    14401 taccgtgggt ggcgtgtttt cgcaggatgg tgaaatggga tctattcgtg gaggtcgtga
    14461 agatggtacg gtaatgtaca tcgacggcgt ccgcgttcgc ggttcttctt ctctgccgaa
    14521 atctgctctg gagcaggtaa ccgttatcac cggtggtctg cccgccaact atggcgatgc
    14581 aaccggtggg gttgttaata tcacaaccaa gggtgccagc cgtacttttg gcatgggcgc
    14641 cgaacttgtt acatcgaagt tccttgatgc atacgattat aatttgcttg gtcttttcat
    14701 gcagggtccc ctgctgaaag gaagagattc aacacagaat accgcgctgc ttggttattt
    14761 ccttgctggc gagttttcat atgaaaaaga caattatcct ttctttgatg gtctctacaa
    14821 agccaaggat gactatcttg ctcaggttga acaaaatccg ctccgcgaaa ccggacttgg
    14881 ttatggtaca taccagaatc tgcagtttgt taccatggac aaccttgaaa aaatcaaggc
    14941 aaaaatcgaa tgtccttcat acgctgccaa cttgtcacta aaacttgatg tcaggacaac
    15001 atccacaaca aacctcactt ttggtggttc gctcaactac agcaatgggc tggcttatgt
    15061 ccacagctac tcaatgttta attacaaaca gtatcctcag gtaatcagca gcacctggcg
    15121 cgttttcggt cgttttacac aacgctttaa tgctgataca gctagcaaat ccattgtgaa
    15181 aaacgttttt tataatattc aggccgacta ttccaaatat aattatctcg agcaggatcc
    15241 tgaccataag gacaatctgt ttaaatatgg ccattgggga gaattcaata cctataaggc
    15301 aaaatcatat gaattgggtg acattgattc tctgggatat tataatgtat atcttttgaa
    15361 caactattac gataccctcg taacatttga gccaggttca aataacccta tcacttcaaa
    15421 ttacacatcg cagtattatg atttgtatga tttgcatagt ggttattact ccaactatac
    15481 ttctattcag aatgccggtg cattgctgaa cggtcagcaa ccagaatctg tttatggatt
    15541 gtggtcggct cccggaacca tttacaacag ctacaacaaa tctgacgcaa cacagattgg
    15601 ggttaatgcc aatgcttcag ctgatattgg aaaccatgca ttccagtttg gtattcaata
    15661 tgagcagcgt aaagactgcg cgattggata tgcgcctgtg agcctctgga cccgtatgcg
    15721 tcagctggcc aacaagcata ttgaacagct tgacctcagc aatcctgatc cacagacaga
    15781 catgtatggt gttttcaccg acacggttga gtattatcgt ttgtatgatg ccaacagtca
    15841 gagcacattt gacaaaaatc ttcgcgaatc tctcggtttg ccggtagatg gtaccgattg
    15901 gattgatgtg gattcctatg atccttcaac ttacaacatt gaatggttct cggctgacga
    15961 acttctgaat gaaggaaaca gttatgtttc ttattatggc tatgattata ccggtaagaa
    16021 actgaccagt aaacccagtt tcgacgattt ctttaccaag aaagatgcga atggaaatta
    16081 tacccgcgaa atcggagcat ttgagccgat ttacatggcc ggttatatca tggacaaatt
    16141 tgctttcaaa gatctgattt tcaacatagg tttgcgcgtt gaccgtttcg atgccaacca
    16201 gatggttctt gaagacaaat atctgttcta tgaagccaaa acggccgatc aggttaccaa
    16261 ccttggagtt catcctggca atattccagg cagtgctaca gtttatgttg acaacataaa
    16321 caacccgaca caaattcttg gctaccgtga tggtgatgtg tggtacaacg cacaaggtac
    16381 tcagattact gacccgtcca cgatttcaac ttccagcggt attgccccgt atctggttga
    16441 tccttcactg acaaaaccga cttcagctgc gtttaaagat tacaaaccgc aggtgaccta
    16501 tatgcctcgt atttcattct cattcccgat ttctgacgaa gcattattct ttgcgcacta
    16561 tgatgtcact tcaaaacgtc cgacctcagg tctgcgtctt gatccgactc aatattactt
    16621 cattcagaca aatgctcagt ctttcatcaa taatcctgat ctgaaacctg agaaaacagt
    16681 tgactatgaa ttaggttttc agcagttgct gagcatcaag tcctcactga aattctcagc
    16741 ttactatcgc gaaatgcgtg acctggtgca ggcctatcgt ttctacggtg gatatccgat
    16801 tgattattat tcctataata atattgactt tggtactgta aaaggtatga ccattgctta
    16861 tgaccttcgc cgcagcgcta acgtctggtt aaaggccagc tatacactgc agtttgccga
    16921 tggtaccgga tcgaactcat cttcaggtat gaaccttgtt cgtacaggtc agccaaacct
    16981 gcgtacgctg aacccactcg attatgaccg tcgtcatgct ttcacccttg ttcttgacta
    17041 ccgtttcggt tcaggaaaga attacaacgg accgaaaatc accaaaaagg taggtgatgg
    17101 aaaagtcaaa gttactccgc tgctccaaaa cactggtgtg aactttactt tcacaggtgg
    17161 ttccggaact ccctacagca aatcaagcaa catcatttcg agtcagcttg gtggcggaag
    17221 ttatcagctg cagggtatga tcaacggcag ccgcctccca tggtcgttcc gcgttgattc
    17281 acgaattgac cgcgacattg atgttaaaat cggaaagaaa gacatgacat tgaatgtgta
    17341 cctggaaatt ctcaatgtcc tgaacagcaa gaatataatg agcgtttatc gttcaaccgg
    17401 taacccggat gatgacggat atcttgctgc tgctgaatat caggcaggta tcgaagcaca
    17461 ggtaaatgaa gaagctttcc gcactttgta ttctattctg gttaacagtc cgtacaatta
    17521 cagcctgccc agacgaatca gatttggtgt tgctttcggt ttctaatcaa gtaaaaaaat
    17581 tgaagtatga aaaagaatat aatcacatcg ttattgttta tcggccttgt tgggctggcg
    17641 atgggctcat atgccagaaa agtgccgata gcagaaaacc gttcccagca ggaaggaaaa
    17701 cagatcagac ttaagtctac tgcagaggcc tgtctgcctg gagcggtgag tacagaactg
    17761 aaaatcaaca atgtccgcgc ccgaatcaat tctggcggtg acatgtggtg ggacctcgcc
    17821 gatgtttccg aatatgaagt tccttatggc tcaaagatga cttccatgtt tgcaggtgct
    17881 ttgtggattg gtggtaccga catcaatggt cagcttaaac tggctgctca ccggtatcgt
    17941 caggttggaa atgacttttt ccctggtcca ctcactgttg atggaacagc atctgttagt
    18001 gccgaaacct gtattaaata cgacaaaatg aatgtcattt atcgcaagga tgttgatgaa
    18061 tttcttgcat ggcaggccag cccttcggat tatccggatt atgtcatgcc atcttatttc
    18121 tcaacatatc cggccatggg tgatgaactt ttgggtcagt cacgttatct ggcgccattt
    18181 tttgatgcca acgaagacct tttctacaat ccgctcgatg gtgattatcc gtattatgat
    18241 attgataatg atctctgtca tcagccggat tcgactctcg aaaccattga gggtattgtg
    18301 tcaggtggaa ttctcgccga tcaggtttta aaaggcgacc agaccatttg gtgggttttc
    18361 aatgataaag gaaatgttca cactgaaact cagggtgcag ccattggtct tgaaatacgt
    18421 gcgcaggcat ttgctttttc aaccaacgat gaaatcaata atatgacttt ctattcattt
    18481 gaaatcatta accgttcgac ctataccttg caggaaacct acatgtcatt gtggactgac
    18541 ccggatctgg gttactcgca tgacgattat gttggttgcg atgttttacg tggtcttgga
    18601 tatgcctaca atggtgacca gtatgatgaa gacggaaacg gtgcttttgg atatggcgac
    18661 cagccaccgg ccgtaggtat tgactttttt cagggaccgt atatggatcc ggatggaatt
    18721 gacaatcccg cttttgtaaa aaccacagat tcttcaggcg caattgtaat tgagaactgt
    18781 aatgaggccg ttaacggtgt aaactttggt aacggaatca tcgatgatga acgttatggt
    18841 atgcgtcgtt tcgtatatca caataattcg ggaacagttc ctccttacat gactgaccct
    18901 gatattgcca ttgagtatta taactttctt cgtggttatt ggaaagacaa tacccgcatg
    18961 ttgtatggcg gcaacgccca cgtatcatcg ggtgcatatg gccctgagtg tgattttatg
    19021 ttcccagacg aaaccgaccc ctgtgattgg ggtacaggcg gtgttccacc tgccggacct
    19081 agaaaatgga ccgaagaaac agctggcaac cagccctatg accgtcgttt catgcagtct
    19141 gccggacctt ttacattgaa accgggtgct cttaactata ttactgttgg tttgccctgg
    19201 gctcgtgcca catcgggtgg cccatttgca tctgttgagt tgctgcgccg taccgacgat
    19261 aaatgtcagc gcttatttga caactgtttt aaagtagttg atgggcctga tgctccggat
    19321 cttactctgc aagaacttga caaggaaatc attctgttta ttaccaacag tggttccagc
    19381 aacaattatc ttgaacagta tgaggaaggt gatccttcca ttgtttctcc tgaaggtgag
    19441 cattatgatt cattgtatcg ttttgaagga tatcaggtat ttcaggttaa ggatatatct
    19501 gtttctgcaa cagatttgta tgatactgac aaagcccgat tgattgctca atgtgatgtt
    19561 gaaaacgatg tttcgcgatt gattaatttt tattattcag atgaccttgg tgctgttgta
    19621 ccacgtgaag aggttaatgg tgccaatgaa ggaattgttc attcattccg cgtgctggaa
    19681 gatcagtttg ctacaggcga taagcgtttg gtgaataaca agaagtatta tttcatggtg
    19741 attgcatatg cttttaatga gtatgaaaag tattcacagg atcctgctta tcagattcct
    19801 ggcatagcat ctctttatgg acagcaaaaa ccgtatctgg ctggtcgtaa gaatatcgaa
    19861 acatatgtgg gaattcccca catgccttca ccggaaaaca gcggaacttt aattcaatcc
    19921 aattatggcg atggtccgaa aatcactcgg attgaagggc aaggcaatgg cggaatgatt
    19981 cttgaaatga ccgaagccag ccgaaatgct gctttaatta ataattcgat tttgactcca
    20041 acgtatgaaa acggatttgg tccgcttgac attaaagtta ttgatccatt gaatgtaaaa
    20101 gaagcggact atgttcttcg cttcgatact ttgagtacta ttccggacag cttgcatctt
    20161 gcccgttgga ttcttgaaga atatgacctg agtggaaatc tggttgccag ttatttgtca
    20221 gatcattcca ttgaaacaaa gtttgaacag atttttgttg accttggaat ttcagttact
    20281 cttgcacagg cgcttgctcc tggtgatcag actcatccga ccaatggatt cctgcaggcc
    20341 gacattattt atgaagatcc tacacgcatg tggcttagtg gtgttcctga tattgatgga
    20401 ccgtcggttt ataactggat tcgttccgga acgacagact cagatgacaa tgatcaggac
    20461 gactttatca tcaacggagt atttggtgat gccggacagt attacgaaaa actgctgggt
    20521 ggcacctggg caccattccg cttgacttca ttctcgactg ataatcctgg tatcgccgaa
    20581 tcatctgtgc aggcatggat ttcgaacacc aataagttga gcaatcttgc cagcattgat
    20641 gttgtattca ctcctgacaa gagcaaatgg acccgttgtc ctgttcttga aatgtgtgaa
    20701 gataaaacgc tttcagaagg cggagctgta aaacactcat tgcgtaagca tgcttctgtt
    20761 gataaaaacg gaaataccgg cacccctgaa gcaacctacg atggttatgc aatgggtatg
    20821 ggatggttcc ctggttatgt catcaatatt gaaacaggtg aacgtttgaa tgttgcattt
    20881 ggtgaagata gctggctgat aggtgaaaac ggtcgtgata tgctgtttaa tccgacggca
    20941 aattacgaag cacttaccca gactcttttc ggtggaaaac acttcctgta tatctttggc
    21001 cataagggtg ataatgtgaa tgattgtcct gcttatgatg gtggtaaatg gcttgctgac
    21061 aagtataacc tgaatacaac tgtagcatgg cgccatattt attcctgcgt tatgtacact
    21121 tctattccgc tgtctgttcc aaacgaagat tggctcagca acgaagcacg tgttcgtatc
    21181 agggtttcaa agccatatgc aataaattat tctacatatg gaagtgcaac tgcacaaaac
    21241 aagaattatc cgatgtatag tttcaacaca tcagatattg ctaccaaaac caatgacctt
    21301 gaaacagcaa aaacagcact tgatctgatt aatgttgttc cgaatccata ttatgcaaac
    21361 agcggttacg aagagactca gcttgataac aaggtgaaga tcacaaatct tccttcgaaa
    21421 tgtaccgtga gtatttacac tgttgatggc atgttgatac gcagatacac tcgtgacgat
    21481 ccgaattcaa cctatatcga ctgggatctg aagaattctg ccaacattcc aatttcggga
    21541 ggtttgtatc tgattcatgt tgatgcaccg ggtgttggtg aacgcaccat caagtggttt
    21601 ggttcattac gtccgattga cttgaattca ttctaatgtt aactactaaa gagagaaagc
    21661 aatgaaaaat atagtaaaaa atctgtttgc aataatgctc cttgttgcat tgacaattcc
    21721 ggtcaatccg gtgtttgccg gcaacgaaga ccgagctgga gaatccggcg caggcgaatt
    21781 gctcataatg ccatttgccc gcagttcagg atggggcatg tccaatacct catgcatccg
    21841 cggtctcgaa ggcatgtatc ttaatgttgc cggtacagca tttaccaaat ctaccgaagt
    21901 gatgtttgcc cgcactgaat ggctgaaggg ttcaggaatc agcatcaatt catttgggtt
    21961 gacacaaaag gtgggtacat cgggtgtgat tggtcttggt gttatgacca tgggcttcgg
    22021 tgacataatg atcaccaccg ttgatctccc tgaaggtggc attggtacat tcaaaccatc
    22081 ctttatgaat attggcattt catacgccaa agcgttttca aatgcaattt atggtggatt
    22141 ggttctgaag gttgtttcag aatccatcgc agataaccgt gcacagggtg ttgctattga
    22201 tgccggcatt cagtatctga ccggtgataa tgaaaacatt cattttggta tagctttgcg
    22261 caatatcgga ccaacgatga aatttaccgg tgatggttta tccttccgtg gtaatatgaa
    22321 taattcagat gttctgatga cggttgagca gcgttcagtg gcattccagt tgccggcttt
    22381 gctcaatatc ggagctgcct atgatttcat tatcaacgat gctcatacca tcacttttgc
    22441 cggtagtttc acttccatgt cttttggcaa ggatgtttat gccggtggat tgcagtacaa
    22501 ctttaacaat tacctcatgc ttcgcggtgg catgcagtac gaaaagggtt tgttcgactc
    22561 tgatgaacgc acctcggcct ataccggccc gactgcaggt atcacaattc aggttcctct
    22621 gaacaaggat aaaggttcaa cattttctct ggattattct tacagaacat ccaatccttt
    22681 ccagggaaca cattgtatcg gagccagaat taacctgtaa gcgctaaagt aaaaggtaat
    22741 taggaaataa ttcgtaaatt tgtcgagcaa gagggcctgt cataaggtct tcttgttcgt
    22801 tttatacaac ctaagagaaa gcaatatggc aaagcaaacg tacattacca aggaagggct
    22861 ggaaaaattg aaggccgaac tcgaccaatt gatgaatgtt gaacggccta agatttccaa
    22921 acaaattgct gaagcacgtg acaaaggcga tttgtcggaa aatgctgaat atgatgcagc
    22981 caaggatgct cagggaatgc ttgaactgaa aatttcgcaa atgcaggaaa ttgtcagcaa
    23041 tagtagaatt atcgatgagt ctaaacttga taattccaaa gcccgtattc tttctacggt
    23101 gactattgaa aacaaaaaga caaagcaaaa aatgacctac actctggttc ccgatcagga
    23161 agctgatttg aaggccggga aattatctgt taatagtccg attgcgcagg cattgcttgg
    23221 gaaaaaggtg ggtgattcgg ctgatgttaa ggttccggcc ggtgttatca gtcttaaatt
    23281 actcgatata aaataagaaa atggcttctg ttttttcaaa gattatcgcc cgcgaaatcc
    23341 ctgctcatat tgttgcagaa agtgcttgct gcatggcatt tctcgacatc agtccgctga
    23401 attacgggca tacgcttgtt gttcctaaaa ctgaggtcga ttatttgttc gatctcgaac
    23461 cgcagatgct ttcggattta atggtatttg cacaacgtgt ttccagagcc atcgaccagt
    23521 gtggatgggg aaaacgtgtg ggtgtggctg ttattggtct cgaagtccct catgctcata
    23581 ttcaccttgt tccaatcaat ggattgcatg atctcgagtt ttcaaatgaa aagcttaagc
    23641 tgtcaaacga ggaattgact gcaattgcgg aacagatccg aagcaaattt atttaagaat
    23701 tttgttgggg ttttcccgca gaaattgatc ccatgagttg ctattctttt ttctggtggg
    23761 agccgatgtt gttttgattt tttgcaatgt gtatgaaatg gctatagcag cggcatcggt
    23821 ggcgtcggtg ttgagttttg aaatgtcgta accggttatg tttgctacca tggcagctac
    23881 ctgttctttt gatgcagagc cctttccggt aatggcttgt tttacgcggc gtggcgtaat
    23941 ctcaacgatt tcaagtccat gttgcatcgc aaccgacata acagaaccct gagcgcgtcc
    24001 cagttttagc atggactgca cattctttcc aaaaaaaggt tcttcgattg ccattacctg
    24061 aggtttgtga gtgttgagca ggttacttgt gaattcaacg attccgcgaa gcttatcaat
    24121 gtgcgtttca tattttgaaa gtctcagaca accgatggta acaatggcat aattgtgacc
    24181 gtcgtatttc agcaaacagt atcccataat gtttgtgccg gggtcgatgc caagaatcag
    24241 ttgttcggaa ttcatatcca aaagtagtat aaaattcaaa agtgaaggcc gatacattgc
    24301 aggattcatt acatttgtgc ctatatttca tgttttgaac aaaaaagcca ttaatattgt
    24361 tctttctatc attattactt cacttatttt agtgtttatc ggttatttga ttcacgaccg
    24421 ccttcaatcc atcgacattg ggtattcact ttccattctg tttgaatctg gcagatctgt
    24481 agtgctttta gtgttggttt ttttgctgat gcctttgaat cttttttgtg aggcattcaa
    24541 gtggaaaacg cttgcagaca gcattgagcg aatctctcta atgcgtgctt tcagtggtat
    24601 tcttgccggt attaccgccg gattgctttt gccaaacaga gtgggcgagt ttgccggaaa
    24661 agcactctca ctatcgcttt cgcagttctg gaagggttct gtgcttgcaa tattgacttc
    24721 gatggcgcaa cttatgatta ctttgattat ggggtttttg gccattattt attttgggcc
    24781 tcaactgaat tattttttcg gtatcagaat ttcattttta cctgtggcca tttctgtggc
    24841 gggaatcgca ttactgctgt ttctgttttt taatattcag gcggtcgcat tattatttaa
    24901 gcgatgggaa aaattacata caattgttgc tgtgctttct tcttcgggat taagtattaa
    24961 attgctgatt ttgtttgtca gtgctctgcg ttatatggtt tttgctggcc aaaatctgct
    25021 tttgcttgcg cttttcgaag tgaatattcc atgggttgat tctttttttc tgattgcgct
    25081 gatgtatttt accttgtctg ctattcccac ttttgttttg acagattttc cggcccgaag
    25141 ctcagttatg ctcttgcttt ttattgcctg gttcgatatt tcaggattga caattcctta
    25201 tgcattggaa gccaaaatca ttgttacgtc ttttctgata tggtttataa atcttattat
    25261 tccggccatt cccggcttgt tctttttaaa cagattttca gtattacgta aagcaaacag
    25321 atgattgtta ttgttgtcac cataggattt attgcgtttt ttgcatttat cgaagcgctc
    25381 tttgccattg gtattatggc cgattcaaat gctttgttgc gtaagaaaaa tattgaatat
    25441 tcggttgttg ttgcagtccg caacgaagaa gaatggattg aatcatgcat tcaatctttg
    25501 tttaaccaaa gtgttcaacc ttcagccatt atagtggttg atgatcattc ggatgataag
    25561 acgttttgca ttgttcatca gatgattgct caaaatccgc ttttggtttt gctttcgtgc
    25621 gatcacgacg aaaccgggaa acgagccgcc ttgatgaaag gaatatcagc tgtccaaact
    25681 gaagttgttc ttacaattga tggtgattgc atggctcgtc gagactgggc tttaaatatg
    25741 ctttcaaaat tcagtgaaaa taaaaaactt gtttcaggtc cggtgattac tctatccaag
    25801 cgatgcctga atatgcctga atatgcggaa tctttgtttc tgatggctgt gggcgccggt
    25861 gcagcttcac agggactgat gtttcaggca tcgggtgcaa atatgctttt tttaaaaagt
    25921 gattttgtcg atttttacaa atcggcttct ggtgcggagt ttagatgtgg tgatgatgtt
    25981 ttctttttgc aatatattca aaaacaatac ggacgaaaag caagtggttt ttgcagcaat
    26041 tccggtgctg ttgttgaaac gcattcttca gcgggcatta aagaatggat atttcaacga
    26101 gttcgttggg cctcgaaatc acgtggttat tccgattgga ttccatttgc agcaggcatt
    26161 ttggttttat tttcaagcag tgcactggtt gcggctttca tattattgat tgcacaacct
    26221 gagttcagtt tttttctact gcctgtcatt cttgtaaaag tgatgggcga tgttcttctg
    26281 ttgttttcat cggcttttgc atggaaagct ccggtaagag tgctttcatt ttttatgctg
    26341 cctttgtttt accctttgtt tctgatgtca gtcggtagcg caatgatatt tcagaaaaac
    26401 aagatttgga aaggcagagc aatcagttga tgtgtggcaa tagcgcttct gcatatttta
    26461 aatcggcagg tgtagtaatt tttatgtttt ccggattacc ttcaatcagt cgtatcgggt
    26521 atccggctgc ttcaaccaca gaggcatcgt cggtgaaaga ctgtatgtat gattgtctgt
    26581 aagcatcgag tagcagggtt gcctgaaaaa attgtggagt ctggcagagg cggaactgtt
    26641 cgcggttggc ggcttttgag agggctccat tgatttcacg gattgactcg cttaaaggaa
    26701 tgaccggtat ggcagagccg gatttttcgg ctaccatgaa gccctgatgt atgacattag
    26761 ctgtaaagag cggtctgact gcatcgtgga tggcgatcac ttcgtcagcg tgtacaaaag
    26821 caagaccgct tttcacagag tggaagcgtt cgggtccgcc aaaagcgatg tttattgaat
    26881 catgaaacgg gataaatttg tgacattctt tccaatgtgg cagatcgcgt tcggcaataa
    26941 caattgtgat gcggctgttg ggtacaattt tataaagccg gttgatggtt acagcaataa
    27001 caggggtttc attaatcttc aggaattgct tggggatagc ggaattcatt cgtttcccgc
    27061 tgccaccggc aacaacgatg atatgaaaag gtgttggcat caaataatca gcatggcatc
    27121 accgtaggtg aaaaatttgt atttttcctt gacggcggtt ttgtacgcat ccatcagtag
    27181 gggatagccg gcaaatgcag ccaccatcat catcagcgac gacatgggga gatgaaaatt
    27241 ggtaaccatg gcatcggcaa cgctgaaatc atagggtgga aaaatgaatt tgttggtcca
    27301 tcccttaaag ggtttcaggt acccgttgat ggtgacactg gattcaatgg ctcgcatgga
    27361 tgttgtgcca actgcgcata ttttcccttt gcgttctttc acttcattta ctttatcggc
    27421 tgttctttcg tcgataaaaa attcttcaga atccattttg tgtttggata ggtcttcaac
    27481 ctcaatggct ctgaaatttc cgagaccggc atgaagtgtt atttctgcaa aatcgactcc
    27541 ctttatttcg agacgtttca tgagttcgcg cgaaaagtgc agcccggctg taggtgcagc
    27601 tactgcgcct tcatgtttgg cgtaaatggt ctgatatcgg tctttatcat cttcaattac
    27661 cggccggttg atgagtttgg gcagaggcgg ctcacccata tttttgagca attctttgaa
    27721 tttatcatgt tccatgtcgg tcaggaacct gagtgtgcgt ccgcgggaag tggtattgtc
    27781 aatgacttcg gccaccagtt cgtcgttttc gccaaagtag agtttgttgc cgattctgat
    27841 ttttcgggct gggtcaacca gaacatccca tagttttgat tcggcattga gttcacgaag
    27901 caaaaatact tcaataacag caccggtttt ttctttacgg ccatacagac gcgctggaaa
    27961 cacttttgtg ttattgataa tcatcaggtc tccatcgccg aagtacttga gaatgtcgtt
    28021 gaattttttg tgctggattg taccggcttt acggtctaaa accatgagtc ggctctcatc
    28081 tctgtttttt gaaggaaagt tggcaattaa atcctgcggt aagtcaaatt tgaagtcaga
    28141 aagtttcatt tagtaatctg aattttttac aaagatacat tcgcaaaaag cgaaagtcaa
    28201 tgaataacga aaaaaagccc aaaatagtat ataaacgccc ctgaatgtca gagaaatgcc
    28261 gagtcgggaa tttcgtcaat cgttaaatca tttgtccaaa cgggtaaatc ggactaattt
    28321 tgcaaaaaaa ttcctgtcat ggcttatatt accaaagaaa agtttctttc agcggagtgg
    28381 ttccgtgttt atttgttgat tttgatcggc tctttcatta tggctgccgg ttttgttttg
    28441 tttatttcgc cctacaaact ggctcctggc ggtgtgtatg gaatagccat tgttttgcat
    28501 tatttaatta atactcccat tggacttatt ggaattgcca tagacattcc gttaacaatt
    28561 attgcaataa aaatactggg gccgaaattc ggcgtaaaaa cagtggctgg ttttgttctt
    28621 ttgtcgggat ggatatcgtt acttgaattt acatggggtt ataagccact ggtagaaggt
    28681 gatccgcttt tatcttctat ttttggtggc ttgctgattg gttttgggtt ggggttaatc
    28741 ttcaaatcga aagccagttc tggcggcagc gatattattg ccatgattct gaataaatac
    28801 acaaaaattc cggttggcca attgctgatt gcagttgatg cagccattgt aatgatcagt
    28861 ctggttgcct tcgacgactg gaaaatccct ctgtacagct ggattgtcat ttttataacg
    28921 ggtaaggtta tagacgctgt cattcaggga gttagttatg acaaaacctg catgatcatt
    28981 accgacaagc cggaggaagt cagcaacaag attctggttg acctgaagcg gggcggcaca
    29041 tttatccggg ccaaagggat gtattcgggg caggaaaaag atatgatcta tactgttgta
    29101 aacaggcgag aagtagccat tttacaagat tatatccgtc agatagaccc caatgcattc
    29161 atgtcggtta tcgatgccaa cgaaattgtt ggaaacggct ttaaaccttt ttccgaaaag
    29221 gggcagtagc cactgtgcta ttttgttccg attactgtta gtgttgattt cgccttcatt
    29281 tggccatttc ttccaatgac attcactatg tattctcctg ccgcaatgct gcggatatcg
    29341 atagttgtac ttgtttcatg attgctcagg tgttgtctca gcatgatttt accgctcata
    29401 tcgcaaatgg taatctctgt gcagttttgc ggaatattgg cgatctgaag caaatcggag
    29461 cagggatttg gaaatatcag gaggtcagat tctgaccccg gctcagcaat gccgctttga
    29521 ttcggttgcc ctacagccag agcgtattct cccagcaaac cataaggcgt tttgagaaag
    29581 ccggaaaaat cgttgcctgt ttttgtaaaa gggaatagct gccagtcata gcccggacct
    29641 ggcctgtaga cgagaataag ggaatcggtg ctggcattct gagggagcaa cccgctgtcg
    29701 aaaaagatgt tggtacggtt ataatcgaac cgcagctgat aatccgtacc ggcaggtaca
    29761 attcctgata tgcgccagta gcggctttcg gaaatccgga aaatatccgg atttgaaaca
    29821 acggggtccg gagccacttt gtgatgttca gcacgcacaa aaatgctgtc ggtgagattg
    29881 tttacaaaaa tatcggaata ggcatagtcg aacgaaacgg ttgcaggcgc cgatatcacc
    29941 gagttgtagc ttacaatggc atcggctgtt ttttcattca aatcagtaat taccaaggtt
    30001 ggtataaaag ggacgttgaa aacatctgta ccgttttgtc ctgaaaagct ggttgtaaca
    30061 gtatgtttgt tccataatga atccaaaaag gtcagttcaa gacggttgct gttggcgtat
    30121 tgagtggtac cgtataattt ttgtttcaga aatactgtta ctgttgatcc tgttccattg
    30181 gggacaacat gcattgaatc tacatcgaag tgcggaaatc cttcgcgcag aacccatgcg
    30241 tcaaagaaat cagtgaggtc cattcctgtt tctgcactca gaatatcgcg catttgaata
    30301 atggtggcgt tgccgtattt cagactatcg aaatattgtt tcaatgcagg aaagaacaga
    30361 gagtcgccaa tataatttct tagggtatgc gctaccagag cacctttgtc ataaacagtt
    30421 gttccatagg taatatgatg tggtacgttg ccgacgggat aaaacccgcc atcgtcaaca
    30481 tgagcaaaac gaagaacaga agcaagcatg gtgcgaatgt aatcttgcat ggctgctgtt
    30541 ccgtaaagac tctcctcggt gataaattca cagaaaacag cccagccctc gttgatccac
    30601 atttcttcgg ctgttgcaca ggttaccaga tcgccgaacc aatgatgcga taattcatga
    30661 taaatcagtg attcatacga agtgttgcca gtgatggtag cattgggcaa agcaatattg
    30721 gtggtgtgtt ccatggcccc tgaattaaat ggaactccta cataaccgac tcgttcccat
    30781 ccataaggcc cgaagcggtt ttcgaacgta gcaaggatat ctttgagatg aatgaatgtg
    30841 cccggaactt ttgacacata cgatgatgaa acataaatat caatcggaat atttccgttg
    30901 atgctgttga aggtgtctgt aacgcacacg tagggcccga cagcgattga tgccagataa
    30961 gtagggatgg gctctgccat tgtccatgtc catgcaatgg aatctgcatt ttcgatatgg
    31021 gtggaatcgg tgagcatgcc accgcaaacg gccatctgat tggtgtcggt gcggacaata
    31081 atttcgtaaa aagccttatc ggtaaaatcg tcaatgcatg gaaaccagac gcggccgtag
    31141 ttgtgagggt tgtcttcgaa gcctacgccc agattgtagg catatggact ctggaaataa
    31201 aatccgcccc agccgctggg atcaacaaca ggagacccgt ggtaaaatat tttcagatgg
    31261 aaagtatcgt tgccggccaa tgcaaccggg ctctgaatca tgagcagcgt gtcgttgtag
    31321 gtccaggttg tattcagcgt tgattccaga taaacctgat caacattcag ctccagcagg
    31381 tcgagcggaa tatattgcag attgttcacc ttaggctgaa agtatatgtc ggtatagcca
    31441 tacagctgtt ttgccgaaat atccaaagta tcaatgttta tggaatatct gattacatca
    31501 atgctgtcgc tgccgggtga tttttgttgc gcggatgcag cagcagaaat gaaaagaatc
    31561 agaatcgcaa tgaaaaaagt tttcatagtt catattttgc aaaagcaaat gtaaggaaat
    31621 tccgtggttg tgtgatgtgt ctatccaaat cagcgcccta cggacgacag aatgctgggt
    31681 ttgtcttatc tctccgactc ttccaggtcc tccatttcat ttcggactct ggaaggtcgg
    31741 aagcgagttt cagagcgagt gaacagtaca agaacgcagc taaatcatgc tcaatgctcc
    31801 atgcgttgtg caatgtgcga gcattttggt ctgccttcag cccgattttg tataattatt
    31861 ttgaaacata attgaatcat cgcacaaatt cgtacttttg ttacacttaa cactcaatta
    31921 ctgatgaaaa gacttttgtt ttttattctg ctgctggctg ctgttgcagc agaaggacag
    31981 acggtctgga cgcttgaatc ctgcattcag tatgcaaggg aaaacaacct tactattcgc
    32041 ttgcagaagc tgaattccga aattgcaaag ttgtcgctga cccgcgacaa ggcggccttt
    32101 ctgcccggtg tttcggccgg agccacgcat tcctacaact atggccgtac cgttgacatg
    32161 tacaccaacg agtttgcaac cgaccgtgtc cgctcgaaca atttctatat tggcgccaac
    32221 attacgcttt tcaacggctt caatctgatc aattcataca aaaaaagtca gctcgaagca
    32281 gaagccgcta tccttgatgc acagcagact tcggatgata tttcgctttc cattgcaacg
    32341 gcctatctgc aggttttgta ttccatggaa ctggtaaaca atgcgcagag ccaacttgac
    32401 atcacattgc ttcaggttga taaaaaccga aaaatgtatg cagccggatc agtctcacag
    32461 ggcgctttac ttacaatcga agcacaggct gcctcggaag aaagtcagtt ggtgagcgcg
    32521 cagaatcagc tcgaaatgtc gtacctcact ttggcgcaaa tgctggatct gaaggacatg
    32581 agcggttttc agattgctgt accggatatc agcacgcttg acaccagttc gtttttactg
    32641 atgaatccgg ccgatgtgta tgcatttgcc gaaaaaaatc agccaggact tatggctgct
    32701 caataccgtg ttcagattgc agaaaggaac ctgggtattg ccagagccgg ccgctatcct
    32761 cagcttacgc tcaacggatc gcttggtacc ggatattcgg gcgccagcag aagaatgaca
    32821 ggatacaatg taaacggagt tgataccatc ggtttcacaa gcgaatcact gacggaatat
    32881 gtgatgatgc cttcgcttga ttacacatac gaaacaacgc cttttcagga tcagatcaac
    32941 gacaacatca acaaatcaat cggactgcaa ttgacgatac cgattttcag tcgtctgaat
    33001 actacactgg cagtgcgtca ggcgaaaatc ggcattgaat cggccaccat taattttgag
    33061 caggaaaaac tgaatctgca gaaaacaatt cagcaggcct atgctgatgc atcgggtgca
    33121 tacagaaaat attcggctac caaaaagcag gtggctgcgc tcgaagaatc tttcaaatac
    33181 atggatcagc gcttcagtgt gggccttgcc acacccattg attacagtga tgcaaaaaac
    33241 aaactggcca cagcaaggtc ggaaatgctg caggcgaaat acgaatttat tttccgcacc
    33301 aaaatactcg actactattt aggcagagaa atcaggttgt aactcaaaaa caacggaatc
    33361 atggcactga aaaaaaagaa caaacgaatt atcatctgga gcattgtagg ccttgcggtt
    33421 ttactcatca ttctgcgtaa ggcaggtgta atcggaggcg ttgaaaccat tcaggttgca
    33481 gttgaaaacg tgactcagcg cgacattact gaaaccgttt cggcgagcgg aaaaattcag
    33541 ccagtgcgtg aagtcaagtt gagtcccgat gtttcaggtg aaattatcga gctgcttgta
    33601 aaagaaggcg accgtgttaa ggaaggtcag gtgctggccc gcgtaaagcc ggacatatac
    33661 caggcaaatt atgagcagct cgctgccaac gtgaattcgc agaaagcaaa tctctcaaat
    33721 tccgaagcgc gattggcaca gaccgaagca caatttgtga acactgaagc atcgtacaac
    33781 cgcaacaaga cattgtttga tcagggtgtt atttcgaagg ccgaattcga tgcagcacag
    33841 gcgcaatacg atgttgcaaa ggcggaagtg caggcatcga aacaaacggt caatgcagct
    33901 cgctttaatg tgtacagcgc tcaggccagc atgagtgagg catcgaaaaa cctgtcgcgg
    33961 acaaccattg ttgctccggt cgatggtacc ataagtacac tcaatgttga agtaggggag
    34021 cgcgttgcag gcgcatcgca attcagttcg ggtaccgaaa ttctgcgcat tgccgacatg
    34081 gcagaaatgg aagtgatggc cagtgtaaat gaaaacgaag ttgtgcggtt gtctctgggc
    34141 gatacggcca ttgttgatgt tgatgcatat ccgaataaaa agttcaaggg catagtaaca
    34201 gaaatcggca attcggccaa cgtgagcggt atcagtgccg atcaggtgag taacttcgat
    34261 gtgactatac aaattctttc atcgtcttat gccgacatga ttccgaaagg cgctgttgac
    34321 tattcccctt tcaggccagg aatgtcggct tcagtagaaa ttcaaaccga aaaagtaaaa
    34381 caggttttat cggttccggt gcaatcggtc acaacccgcg atgaatctga gcttatggtt
    34441 gttgatgata agaaaaaaac tgatgaaaaa gataatcacg atggcattac cgaatttgtt
    34501 tttgtgattg aaaatggtat tgcaaagcgc agaacggtga aaaccggcgt tcaggataat
    34561 gaatacattc agattctcga agggctcaaa aaagatgagg ttgttattgt tgcgccgtac
    34621 agcgccatag caaagaaact tcagggagaa gagaaagtgg aagttgtgga tcgcaaaaat
    34681 ctttttgaag taaattgagt ttgattcttc atatagaaac agctacgcag gtttgttcag
    34741 ccgcgctgag ccacgatggc atgttgatgg ctgaagctgt tgctgaaact gaaatgtcgc
    34801 atgcatctac gctcatgcct ttgattgatg agcttttcaa aaaacagaat attgatctga
    34861 aagatctctg cgcagtatct gttagctcgg gacctggctc ctatactggg ctgcgaatag
    34921 gtgccagcac ggcaaaaggc atttgctatt cactcgggat tcctctgatt gctgtcggat
    34981 cgctgcattc agcagcatca ggtatgaaag aaatttcaaa tgcaaaagga accgatctgc
    35041 tgattccgat gatagatgcg cgccgtatgg aagcgtttgc ggctatttac gatgcttcgc
    35101 tcaaggaact cagagcgccg catccgtgga tccttgatca gtcaagtctc gatgagtttt
    35161 cgggcagaaa aatatttctt gccggaacag gagctgataa attgaaagcg ttgtattcat
    35221 cattcagtaa tgttgttttt ctgaatgaaa atgtgcatcg ggcttcattt gcatgcagca
    35281 ttgcttttga caaatttcag cattcagcat ttgaagacac ggcctatttt gagcccgatt
    35341 acgggaagga tttcgttccg ggcaagccgg taataaaagg attgaaataa attttgtatt
    35401 gtatgactcc gtatttttca gtaatagtgc cagtttacaa tcgtccggat gaagtggatg
    35461 aattgctgga cagtcttgtg aaacaaacga acaaagattt tgaagtattg atagtggagg
    35521 atggctccac attgaaatgc gaagatgtct gcaataagta tgttgacaag ctcgacctga
    35581 aatatttatt caaaccaaac agtggccgca gcgaaacccg caactacggc atggagcgcg
    35641 caaatggtga gtattttatc atcttcgatt cggatgtgat tctgccatca aattattttg
    35701 aaatagtgag aaaaaatctg gaggcggatt acattgattg ttacggaggc cctgacagcg
    35761 ctgatgattc attttcaatt gttcagaagg ccatcaatta ttcgatgaca tccatcatga
    35821 ctactggcgg aatccgcggt gcgacgaaga atgtggacaa attttctcca cgttcattca
    35881 atatgggaat gtcgaaagaa gttttccaaa aagtgggcgg atacaaaaac atgatcggcg
    35941 aagacattga tttgagtttt cgcatcaagc aagctggctt taagacaaga ctattccgtg
    36001 atgcatatgt ttttcataaa cgtcgagtga gttttaaaaa attttatcgc caggtaaata
    36061 cttttggaaa agggcgtgta ttgttgtcta aaatacatcc gggcacactc aaagcggttc
    36121 acttgttgcc catgtttttt gtattgggta accttgcgct tgctgctctg gcagccattt
    36181 tcctgaatcc gtggtttttg cttccgctgg gagtttatgt gattgctgtg ttttttgaat
    36241 cgctgataaa aaataaaaaa ctttcgattg cgattctttc aatcgttgca agctactggc
    36301 agctttttgg ctatgggaca gggtttatgg gcgagctcat caccggaaaa gccggtaaaa
    36361 gttctcagga aaaattgtac aaataattca attgtatttt ctggtgaacg gatatttcat
    36421 gatgattatg aaatgtgccc atagtgtgcg gcacagaccg aaatgttttt tcataatgca
    36481 aaaacgctcc ttccacgatt tcttgcgatg ttgtgccgac aggcccgaaa tcatgaacct
    36541 ggaaatgtaa tcgtcaatga acaggttgtg ctttgacacg gcaagtactt ttgctgtcca
    36601 gtcataatct gccgaaattc tgtattgcaa atcatataac ggagcaatgg atttccgcac
    36661 caggattgac tgatggcaca ctaccaatcc acggagcaaa tgcttttttt tcagattggc
    36721 cggtgcaatt ttatggcgct ctcccaatgg ctgatcattt tctccaatca tgagcgattg
    36781 tccgtaaacc acatccgaag acggattcag tcttaatgca ttttctattt tttcaacagt
    36841 atgctcatca aatattttat ctccggcgtt gatgaaccat acaaaatcgc cggtagccat
    36901 ttgcatgcct ttgttcatcg catcatacag acctttgtcg ggctcgctta tgtagcggaa
    36961 atccgatgat ttcaccgctg gatattttcc ttctgcaatt tctttttcaa accgttggat
    37021 tatttcaact gttttatctt ttgaatttcc atcaacaata acatactcaa cgctgccatt
    37081 tttttgctgc agcacgctct gcatggtctt ttcgagaacc ttctctgcgt tgtaggtgat
    37141 ggtgatgatt gaaactacag ccatttttat ttattcagat accactgata cactttttca
    37201 atgccttctt tcaacgttgt tttgtgtttc caccccgatg cgtgcagacg gctcacatca
    37261 agcagtttgc gcggtgtgcc gtcgggcctg gtggtatcat ataccatttt gccttcgtat
    37321 cccacaaccg atttcaccgt taatgcaagt tcattgatgg acaaatcttc accggttccg
    37381 atgtttacat gaatctcttc ggaataatgc tgcatcagat gcaccaaagc atcggcaaga
    37441 tcatccacat acagaaattc gcgcagcggt ttgcctgtgc cccacaaaac aacttccggc
    37501 atattctcaa ttttcgcttc atggaatttc cggatcattg caggaagtac atgcgatgaa
    37561 ttgaggtcga agttgtcgtt gatgccgtag agatttgtgg gcatggctga aatgaaatcg
    37621 catccgtact gtcggcggta gaatttgcac aattcaagtc ccgaaatttt tgcaatggca
    37681 tacgcttcat tggttggttc cagtgctcct gtgagcagga actcttcgcg aatgggttgt
    37741 ggagccattt tcggatagat gcatgagctt ccgagaaaca aaagtttttt cacctgattc
    37801 agatatgctg catgaatcac gttgaattca atcatcatgt tgtcatagat gaattctgca
    37861 ggatatgtgt tgttggcatg aataccgccc acttttgcag ctgcgagaat aacgtaatcc
    37921 ggtttttccg aagcaaaaaa atcgttgacg gctttctgat tgcggaggtc gagctcagtt
    37981 atatcgcgat gaataatatc attgaatccg tgttttctga gagatctgac tatggctgag
    38041 ccgacaagtc cggtatgacc tgccacataa attttttcat ttaattccat gatgctattc
    38101 caggtttttt tttacaaatt caagatcata tttcaccatg atccgaacga gttcctcaaa
    38161 tgatgttttg gtgggattcc agcccagtgt tttccgggct tttgatggat cgccctgtaa
    38221 ttgttcaact tcagtcggac ggtaatattt gggatcaact tcgacaagag tatttcctgt
    38281 tttgacatcg attccttttt cgttttcatt ctcaccttcc cagcgaagtt caatgccggc
    38341 ttcggcaaat gcgagcgtgc agaattcgcg cacagaatgc atctgcccgg tagcaatgac
    38401 aaaatcctca gctgtattat gctgaagaat cagccacatg cattcgatgt agtcttttgc
    38461 atagccccag tctcgcatgg cgttcagatt gccgagatat agttttttct gcaatccatg
    38521 tgtaatgcgg gccgctgcca gtgtgatttt gcgtgttaca aaagtttcac cgcgccgttc
    38581 gctttcgtga ttgaaaagaa tgccgttggc tgcaaaaata ttgtaagctt cgcgataatt
    38641 ttttgtaatc cagaatccat acaactttgc aactccgtag ggtgagcgcg gataaaacgg
    38701 tgtgttttcc gattgaggga cttcctgtac tttcccatac aattcagaag tggaggcctg
    38761 atatattttt gttttctttt ccaggccaag tattcgcaca gcctcaagta cacgaagggt
    38821 tccaatggca tcggtttctg cagtgtattc aggaacttcg aatgaaactt ttacatgtga
    38881 ttgtgctgcc agattgtata tttcgtccgg ctgtaccgat tgaatgattc tgatgatgga
    38941 agatgaatcg gtcagatcgc cgtaatgaag ctcgattttt ttctgatgct tcatgtcttc
    39001 aatccattca tcaaaataca aatgttcgat acgtccggta ttgaagtagg atgcgcggcg
    39061 catgagccca tgaacatcat atcccttttc gagcagaaat tcagccagat aagacccgtc
    39121 ctgacctgtg atacctgtaa tcagtgctgt ttttttcatt attttattca attgattcag
    39181 tagaattatc agctttatta ttgttgcgaa tccatttcca gatcaagaga ccaatgattg
    39241 aaatcagcgc cagacctgcg attacagacc cccataatga tattttctgt ccttttatat
    39301 acagttcatc tttgcaggta aactgaatta catgattgcc tgcaggcacc tgtaatccgc
    39361 gcagaatgta gtttacgcga ataatgggca cgtcttttcc atcaatggtg gctttccatg
    39421 ttttataaaa tacttctgag aaaacggcca gctgattctt tgtgtttgtt gttttatagc
    39481 tgattgcgcc tggagaataa tccatcatta cgattgctgc ggagctatca gctgttgttt
    39541 gtaaagcttc aggactactg agtttatctt tccaaacctg atcgataact gcggtcgtgc
    39601 gcgggttgaa atcagttatg gccacaattt cctcatcggg tccatttacc catttcaatg
    39661 tatcgacaaa ccaggcgttt ccgagagcat cgggattttt ctgcactccg gttccaccgg
    39721 tttccctgtt gggaacaata acatagcggg tattcagcat gttcagaaca ttcacattga
    39781 ttttttcgga aaggtgataa tcgatgatat cctgatagcg tctgagttta gcggggctgt
    39841 agccgccaat ggatttgtgg tagtaagaag tattggattc gttgaaagtg ctgctggcaa
    39901 gattaagtac ccggtaattc gggtctttgt cttcaagaat gattttatcg gcctcggtca
    39961 tctgaaatgt ttttgctttg cgtttgggaa caaaattgtc atttccaaga aaacggcggt
    40021 caacgaccca caggtcaatc agaatgagtg cgccaagccc gatgatgaga taattgtttt
    40081 tgaatcggta tttcagaaat gcccacatga ggccgaaggc aaggaagata aaagcaaatg
    40141 atcgccaagc atcgttgacc atcattgatt ttcggtcggc ctgcagtgag tcaatgagcc
    40201 aatccggata gtttgcatca gatgcagaag aaaagtcaaa caaaccaccg ccaagcactg
    40261 cgaaaataag cgtaaggcct ccggtaattc ctccggcgat atatagtttt ttcagcagcg
    40321 cttctttttt attttcgtct ttgtattgct caataaattc tttgatggct aatatggcga
    40381 gaatggccat ggtaacacca gcgatagtca gagccatggc cggagttctg aatttattgt
    40441 acatcggcag attatcgaaa atgaactgat tgaatcctcc gagattttta ccccaggcca
    40501 gcatgacaga aaggatggtt gcggcaagca accaccattt ttcttttcct ttcacgagaa
    40561 tcaatcctaa aacaaaaagg aaacaaacaa tggctccggc atagactggt cccgatgtaa
    40621 agggctgtgc gccccaatag gtgggggcat atttgcagaa attcttgccc tgagcaccac
    40681 caacaccatt tgccttcaat gttttgtaac attccgattc attgccgata ttgtagtggg
    40741 agctggcgcc atagagattt gggatcagca aggtccatgt ttcaccaatg ccataactcc
    40801 attgatatgc atagtcaata tcaagccctg acccttcttt ttgacctttg ggattatttt
    40861 taagaaccgc accgccacgc atggtttctt tggtgtagtc catgatggta atcattcttc
    40921 cgatggatgg cagaacggcg attcccgcaa cgattaacag aatagcagaa accttgagaa
    40981 aatgttttat tgccttttct tttattgcat agatgaaata ggtaacaaac ataatggcaa
    41041 taatcagcaa gagatagtag ctgatttgct gatgattgta ataaatatga ataccggtaa
    41101 agataagggt tgtcagaatg ccgatcagat attttttttg ataagtgaga atgattccac
    41161 caatgaccgg cgccatagta gccatggcca gtcctttgtt tacatgaccg gcatcgataa
    41221 taatcagatt ataggatgca aaagcataag caatagaccc aacaattgca agccatggtt
    41281 tgacattaaa agccatcagc aggatataaa agccaataag atacagaaag acaataccca
    41341 catgcagccc gggaagattg agccgcagaa taatgctcag atctttgaaa atattggtca
    41401 tgggtggggc ataggtgtaa ttggccggca tgccgccaaa cattgtgttt gaccaatatg
    41461 cgtattctcc ggttttttca taatgatccc tgatatcttt cccccaagcc tgggcacttg
    41521 caacatcgcc ctgtggcagg tctttgttgt caaaaacaac aggagaaaaa tatgcaacgg
    41581 ataaagcaat aaaaatcaaa acagcaatga gatgaggaaa ccaaagcctg atattaattc
    41641 ttttcattga ggtgcgtttt acaaaaatga attgcaaaaa taaggttttt tattgttgct
    41701 ttggtttagt ttgtttatgg tgtatgtttt atagcaatga ttgaaataaa taaatgattg
    41761 aatcattcac agaatgctaa tttcggcata aacattcttt ggtgccctgc ttcgataatc
    41821 atgaaaaatc aataagaaaa acactaacct tgtttgttca ggtttgattc gatgaattct
    41881 tcaatattaa aaaagtgttg ttcaatctcg agtagtcttg aaggatcgaa attccaccat
    41941 tgaatctttt gaaggttctg aatttgttct tctgtgaaac gatactttac aatctgaaca
    42001 gggactccca ctgcaatggc ataaggagga atatcttttg aaaccacggc tcctgcacca
    42061 ataatggctc catcgccaat gcttactccg tcaagtattg taacattggc acctataaac
    42121 acatcgtttc caatggtgat ggtttttctt tcattgagtt tatcggtggt gcacaaactg
    42181 attccattct gcctggcagt tgaataaaac ataggtgcag tgctgatgcc attggttgga
    42241 tggactcccc atccgcataa aagattggga ccaatagaac agaatttccc gatttttgta
    42301 ttgttgatcc atgaattcat tgaaatgtag gtataatctc ctacttctgc atcccggata
    42361 tgtccgggaa tagctatttt gacgtgtttt ccctttttga ttctttttga gccggatctg
    42421 atataactca gaaagacatt gttttctctg aatcgcataa attcaaagaa agtagtgaaa
    42481 aggtctttta ttttaccgaa catattattg atttaagaca ctgatttacg tttaaatgca
    42541 tcgatgaagg attctggagt ggcattgaaa actgtacttt ttgtggtttt tgaatattgc
    42601 tctatttcat gataaccctt aaacatgaaa tagagaaatg aaagcatggt tgccagatca
    42661 aattccattg tccgtgcatt ttttgccgcg tcttctttgt cgtagaagtg aaaatcattc
    42721 agacgaagct tgtttttttc atcaacccac aggtttttca gccagtcgtt ttcgcttccg
    42781 atgaggtata tttcgctata tcccgaatat atagccagca tgagcgccgc aaccaaaaca
    42841 ttctgtgcgc gtggcattcc gagttgtttt ctgtatgcga attttttcag aaaacgaaaa
    42901 ccttctactt ttgttttatt gtagtaacat attgtgatgt tgttgttttt tttcagaatt
    42961 tggttgattt ctttttcctt gcatgcctgc catggcagat aaagttgcat ttcccatgtt
    43021 acttgagaag tcagatgctg ataaaagtcc aacacttttt ccttttgttt ttcttgtttt
    43081 acatcaaacc agaaagccgg atcgcatagg acatatgttt ctggtttgag ttcggcaaat
    43141 atgtctgttg ttcccataaa attaatagca atcagatcat gggtttctga tatgtggttt
    43201 aatgatttta ttgtattgtt caatgatgga ccatttccta aaacgatgca atgcgaatgt
    43261 tttttattta ttttttttgt ttttgtgaaa cgactgatcc acaacaatcg gaataatgaa
    43321 aaaaaagcat caatggaatt gtaaaaccat tggagttgtt tcgggtacat acctgatgtt
    43381 tgtttcttgc aaatgtacaa aatatcggat tattcaatgt tgtattgttt agggtatata
    43441 atcatacatg atttcataaa aaaagcgcct taataaagcg ctttttttga attcttttat
    43501 caagtcatca gttatgcgaa ctcagatcct cataaatgat agtgggataa tcttcggtgg
    43561 tggtgccttc ggttttgatt accccggtac tcagattgaa ccagatatac ttgtaataca
    43621 ccgggtcctc actggtggtt tcaagtatct tgatgcagtt tgtaaaagta cctgcaggaa
    43681 cagtaacggt ttcgttcagt gcaacaattc tgcatgaatc agaaaccgta cccgaagtat
    43741 cggtccatgt ctcggcccag aactcgtcta ccgcaggatt ggcatcacaa aacacaagca
    43801 ttgttccgcc gccttgcgag cagagcattg agaatttggt atcatcggca taccattcct
    43861 gcgtaattgt atacattcct gcaacgttcc atgttacatt tgcatacttc tgagcattga
    43921 tgctattgat ggtaaatgtc tgattgaaat ggggagaccc ggcatccgaa tcatatgtgt
    43981 catatgtcca ggtggcgccg gttttgaaga atgtgaaaac gctgctggtt ggagttgttg
    44041 gtgtattgtt gtctggttcg ggatcatctt ttttgcaccc tgtaaagatg agtgcaacag
    44101 caagcacaag tgctgcgggc agtagttttt tcatatacct gaaattaatg tttgtttaca
    44161 gtttgatgaa ttcgacgcgg cggttttttg atttgttttc cgggttgtca ttgggtccga
    44221 tgggttgact ttcgcccaat ccatccgttt cgatgcggct ggcatcgata ccaaaatcgt
    44281 tgaccagttt tgtttttaca ttggcagcgc gtcgttttga aaggtcaaga ttcatggcat
    44341 catcgccgtc gctatcggtg tgtccaacta tttttatccg tacatctggg ttttccttca
    44401 gaatattggc aatttcgttg agcgatccct gtgattctga ttttacaaca tcttttccgc
    44461 tgtcgaaata aataccataa ctgatcagtt tgccttcggt cagtaatttg ctgcgcatat
    44521 caggcgaagc tgtggtaatt ttcagattgg taacaaaggg gtggctgttg cgatcccagc
    44581 caaaaaagcg gaaacggttg aatttccctt cagcataaat attggttgga acatcaagta
    44641 ctttggcacc ctgatgataa atacgaacac gacgtttctg aacccaaatg ataacatgat
    44701 ttactttttc ttttactatt ggatttttcg atgcatttcc ttcaagccaa tccttcccac
    44761 tttcgccata acctttagat tcccagccat cgggagctaa tgtgatgcga agcccatata
    44821 atccaggata cagggcgtcc tcaatttcgc gcacgttttc cggatctgtt tggtataagg
    44881 aaagaacaat acctctggag taatctgcat cgggtatgat atcaaactca aaaatgtagt
    44941 tgtctggaaa tgcaatgtct tttgaaaagc agtaagttgc atcttctccg ttcaaatgaa
    45001 accatttgcc tggagctata ttgacggttt ttacttctcc actgctgttc gatgtccata
    45061 atgcaggaaa gtcgccgatg gcatcttgtg aaaaatcctc gaagaaaaga attttgtcgc
    45121 ccggaatgaa atcgtaaacc gaataacttt ccagtttcac attgttttct ccattttccg
    45181 aacttgagtt ttcctcaaca ttttcgctct cttcagtgtt ttcgccttca atgttttctt
    45241 cagtttctcc atcttcggca tttttatcct tgtctttgtt ctttttctta aagaccccat
    45301 taatgccctc ttcggttttg tcaaaacctt tgtcaatgga ttcatctgtt ttttgctcca
    45361 gtttttcctg tgccttgttt ttgattttgc ctccaatatc aatctgagca aaagaaatac
    45421 tgctgatcaa taaagcagta agaaggatta agaattgttt catggtcgtt ttttttacaa
    45481 aaatagttaa ttcttggcga aaataatcgt tttgttttaa tttgccgatt attttcgcgt
    45541 gccgctaatt aaaaatcgtg ccaatgtcta tttttctttt ttttgacgaa caatgcccag
    45601 gagtgtaaaa ttcggcataa ttttgttaaa taaatccctg tgtgttaaat tttaaaatat
    45661 tggaaatcag taggtattga aaaataatca aaaaaatggc gaaaatattt aaaaaaaatt
    45721 aagaatgatt gacaaatgca aaataaaggt ttaattttat gctttcaaat acgaacaata
    45781 aaaaccggga agtcatgaat acaaagtttt tagtattatt attgggaata ggatttctga
    45841 tgttttcctg cggcgatgac aagaaagccc agatacgtaa agtaatgaaa ccagtggcca
    45901 gtgttgatct gggaacccag gtttttatta ttgatcctac aaaagacaca ttattaaata
    45961 tggaaagcgg aagttccgtt tttattccgg ccaattcgtt ggtgggagct gataacaagc
    46021 ccgtgaaagg caaagccgag cttaagtatg aagaatacct caatcaggcc gaggttattc
    46081 tcagcggcat tccaatgact tacgacagcg cgggcgaaac tcattatttc gagtcggcgg
    46141 gcatgtttac cattgatgcc aacacaccgg caggcgaaaa actgaaaatt gccgatggca
    46201 agtcggttac tgtttcgcag gtgtcgccat gggctgatct tacttcgggt tacaactttt
    46261 atcagttcga taccgttgcc ggcaaatggc gttatctgac aacccagatt gctgagccgg
    46321 cagccagcga gccccctgtt atcgaaaccg gaaaggatgc cgacgtgaag gtgaaaaaaa
    46381 gtgttcgtac tgtcgatgat tttgttttcg atatcaatgt gaattatact gtttataaag
    46441 agcttgccga catgaaagat atcatgtgga aatatagcgg caacaaggaa tatcccgatc
    46501 ctgaaaaaga acaatggata tttgattata attggcccga aacacagata ttgcgcgatg
    46561 agagccagcc cggggcttat gtgattgtgc ttaaaagcac gcagaagtct ttcaaaactt
    46621 cggtcgttcc tgttcccctt gaagctatgg aagaagagga tgttattgcc cgtgccgaca
    46681 agagtatgga aaaactggga gaacaaacgg cgatgatggt tgaaaatagt aattcttacc
    46741 gtcgcacgat ggaagtaagt tctttgggaa tctgcaactg ggatctgatt tatagattta
    46801 ttgttcccaa aagacttaaa atccgttttt ttgctgatgg tgatattttt aaagaagatg
    46861 tcagaattta tcaggtaaat attgaccgca atgtaacgat tgagtgtttg ccggagaatg
    46921 cacattacaa ttgcatttta tcttcagggg aaaaaataat gtatgtagct gtcagccccg
    46981 aaggtcaggc atatgtaaat aaagaaaccg caattcttat gaatgcatct gaggacgaag
    47041 taatcgattt tgatttgcat aagacggcca aaacgattaa ctccaagaag gatctgaatg
    47101 aattgattgc cggcgtctga ttctcattta tatgagattg ctgcaggtat ttttgttgat
    47161 tttgcttttc aatgccggat tctgtcagga atccggccct ttgtttattg tcaggcaaga
    47221 ccagctttat ggattgatag attcttcggg cagcgtggtg cttgaaccac agtttactgc
    47281 cattagctat gatcctcaat ttagaatttt tattgttacc ggacagtcgg gcaaggcagc
    47341 ttttgcatct tcgcaaaatg cctttataac cggtttttat gacgaagttc aagctgtttc
    47401 gctgtactat tttattgtta caaggaatgg tctgtgtggg gtggttgacc gtagtggaaa
    47461 gatgattgtt ccgattcagt attcctcaat ccgcagatca gacagcgaca ggtttttttg
    47521 ttctaataac gcatcagttg ctgtttacaa caacaatggt tctttggtag taaacatccc
    47581 atccgaagaa attattgaat attgcgggta ttatattttt aaaaacaggg attatcgttt
    47641 tggggttgtt tctgtttcag gtgaaaaaat gattgaggat gtttattcga attttctgac
    47701 gggggatggt cttattgaac ttcgaagcga agacagcact gctattcttc atacatcttc
    47761 gggtcgggtg gtacatggtg ctgctggttg cagcttcagg tttattaagg atgcttcaag
    47821 aaacaacctt gcaacacctt tttatttgct tgccaatgga agcagccgtc aatggcactc
    47881 ggtttacagt cataaaaatc tcgatgttga tttgtctgag gcagttattt atgctacagc
    47941 tcttagtgat ttgtatctcg taaatactac attcgagtgg ttgattgaca ataatggagt
    48001 ttcggttaca gatgaaaagt atgaggatat tttttatttc cgcgatagtt tatttgtcat
    48061 tgaaaacaat ggattgcgtg gtttattcaa acctggaacc ggcgttattc ttcagccgat
    48121 atataaaagt ttcaggtctt tgacggatac aataaccgga aatcgctgga cattggcatt
    48181 atctaaccct gatggataca gatttcttga tcagaatttc agggaaataa acgaccagtt
    48241 atatttcgcg gtcaatcttg ttcatggtag gggaattatt gtcaatatca gtctttcaga
    48301 ggtaattttg cttgatactg atggccgacg ggttggtaat gctgtttatt ctgccattca
    48361 accattgtct gatggtttct ggaaagtgta tcgaaatggt ttttgcggtg tgatggattc
    48421 tcaggcaaaa cttttgacgg aaatatgcta tgatgatatt acgatgcata attcgcgcct
    48481 gaagtgtgtg agagaggacc gcattgaata ttatagatgg aatggttcat cgtttgagcc
    48541 cttgataaat ttcagcaatt tcagacagct tgatgtaacg ggtacacagt tattgtcttt
    48601 gattggaacc cgcagttcgc aactggcttt ggtgtggagg cgcgattcgg ttttgaaaaa
    48661 attcgggctg tatgatgtgt tcaataagag gtttcaggtg tctccggtgt atgattatgc
    48721 aaaaatggat cagttgcgcg acatctcttt gacaggaatt tttggcgaca ccagttattt
    48781 ccgcttgggt cccttaactt ttatggggag gattcgtttt ggggtgattg atcataaaac
    48841 agggcaggta attgctccga atgaattcat gtttatttat ttcaatcagt ttcagcggca
    48901 cttttctgag agcaacagtg atctggtaac tccttactat aattcagacc gcaattttgc
    48961 tgttgccatt gatacggatg gaaactggcg tactatttca tttaccggaa caacatataa
    49021 tccggatgtt cagtttattg atgtcagtag tgggaccaga aaagtctggc gttttgattc
    49081 ttgctatgtt tcaaataaac gcaaaagaat tacagataaa aaaatttgca atgttaatgc
    49141 tgcctttgct cctctcttgt cgtttcttaa acctgccgat gcttatacag aggcagcaat
    49201 gaaggacaaa agattatcgg tttttgcacc cgatgcagat tcgctggttt tgcaaaaagg
    49261 ccttgccagt cgtcttgatg agggtcttga tgatattcct ctggtgtatt tccgcccatt
    49321 gtttgtttac aaatatcatt atccgtcgat tctggagtat cgtaaccgtt tattttgcaa
    49381 tgtactgata tttgaaagcg agcgtcgttt cttttgcatc gattcaactg gttatgtcat
    49441 tacagatacg gcttattcgc gcatatggcc gttttcggag gatttggcga tggttaaaat
    49501 gggttcgcga tatggtttta ttgacccgca tggggcgctt attattgaaa acaagtacag
    49561 aaaagcatct tctttttctg aaggaaaagc agcagttagc atcaaggggg gacgctatgg
    49621 ctatatcggt accgacggcg cgtggataat cgaaccggcc taccgcgagg cattggcttt
    49681 cagcgaaggg ctggctccgg tgaagccggg aaatttattt ggttatgtgg atgaatcagg
    49741 aaagatgatt ctcaaacctg cctggttgcg tgccaattct tttatcaatg gaactgccgt
    49801 tgtttttaat aaaaggggat tcggattgat tgacaagtca ggcaatgagt tgcttaaatg
    49861 ccgatacaac cgtataacac cacctgacac caatggcaat cggatgattg ttaggaaggc
    49921 gtcgcaactt gtaaatgctt cgggtgatat aattacaaaa aaatcgcgga ttatttccga
    49981 tgccggcaat ggttattata gtgtgaaaaa agtggtggga ttcagtctta ttcgttccga
    50041 tggttccgaa gtggcgcatt ataattccga caggcctttg cttgttggtg atgaaaaagt
    50101 gatagtaaca aagagaaaca ggttgtatta ttatgatctt gctgcaaatc agcttctggg
    50161 gccttattat aaagcagccc cgtttgttat gaatcgcgca ttggtttcaa tccgcgatga
    50221 aacgatggtt attgatagta gcggaaaaat acttaaaatt ctttatcgga agcggatgtc
    50281 ttttgtaacg tcttttgatg aaaacgggct tgctgtcgtt cgcgcaggca atacttttat
    50341 gctgattgat gaaaccggtg ccgagtttta taatggcacc atcaagccac gctatgccgg
    50401 caacggtgta tatattttat ttcatgacgg ttattttttg tataatcaca gaatcaggac
    50461 ttcgtatcca ttcagtaaat ggagtcttat ttccatgcca agctgcggaa ggtttgttac
    50521 gggggtgaac ggaaaattcg ggttttcgga tgtttatggt cagtaccatg tcaggccgca
    50581 atatacaagg attgaaagag tcagtgaagg tatatacagg gtgtattacg gcgataaaac
    50641 cggctatgtg cgttacgatg gcgaaataat ctgggaattg agccgatagc aacaaataag
    50701 cactcgaaga gtttcaaacc catagaggtt tagagcttaa tgcccattag caacttcgcg
    50761 tgcaaaatcc catgaaagat aattttcgta gctcatgcat acatgctcgg caaactgaat
    50821 ggcggtgcgg ataattttgt cccagttttt ttctggcaat gtttccaggt cagccggaga
    50881 aatatgagtg tggctcaatg cataaagcag accggcattg aaagtgtctc ctgcgccaat
    50941 ggtgctgata accggaatgt cttttacagg atagtgcttt tgaatttgcg gtgtgcagag
    51001 tgttacgccg tgcttgttgg ctgtcattat cagcacatgg ccgccttgtg tctgaacaaa
    51061 atcatatacg atttttggat cggatgtgcc aagaatatta ttgaaatctt catcagaacc
    51121 tttggtaata tgtgccagac gcatgttttc gctgatcatg ggcataacct gatccttaat
    51181 tttcatgtga tttttacgga aattcggatc gtacaatacg atgcttccga catttcggca
    51241 tacatgaaga aaagcggtaa ggccttcgcg gatttcttct ttaatgccat aaaaactacc
    51301 aaacatgacc agatcgtttt tatcgggctc cggataaatc agttcggctt gtccttcttt
    51361 gcgtattttg taaaaagaat attccgcatc gttgttgtcg tttaaaactg caatggcaat
    51421 gcgagaaaga ccgttgttgt aacttttgat gtagtcgagt gaaacctgat tgcgaacaag
    51481 aaatttttca atcaatctgc ctatggcatc ttttgcataa tcgccaacca gtacaacgtt
    51541 ttcgccaaga cgtcccaagg aaacagaggt gttaagcatg gctccaccgg gggtcccttc
    51601 aacgggctta tcttctttga ataatatatc gtaaacggtt tcaccgatgc aaataaaacg
    51661 tttctggggc aatttcatac ggaaaagatt tcaggaagga tttgtactgt tgtattttgc
    51721 aaagattttt gcaatgtatc caataagcgg agaaatgaaa atgtaaacaa taatcagtgg
    51781 cgctatggcg gccagatcaa gaaagatgaa cagcggaaca gcaatcatca ataagatgat
    51841 gcctaatgct atgctttttt ggtcgtggag tttgagcgat aacaagggaa atgggatcag
    51901 catcaatgct gcaaatgtaa atgatgctat ggagagcaga atgatagcat tgtcaatccc
    51961 atacaattcg gcccagtaaa gactgatgca ggcaagtgcc atgccagcag ccggcgaggg
    52021 gagtcccaga aaactttttg tttgtcgttc gtcggtatta aaccatgcta gtcggattgc
    52081 ggcagcaaga gcaaagagca gcggaaaagc aaaaaacatg gtttgattgc aaaacaaatc
    52141 attgcatggt tcgctgtatt gctgcatggt gcgtatggta acaactgctg gcgccacgcc
    52201 gaacgaaaca acatcgcaga gagaatcaaa ttcttttccg aacgacgatt gtgcattcag
    52261 cattcgtgca accatgccat ccatgaggtc aaatgctgct gcaatcagaa tgcagtaaag
    52321 tgcatattca atgtagtacc atgatcccag tgtcgcaaca atagcgcaaa gtcctgccag
    52381 catgttgcct gctgtgaaaa tattgggtat ggctgttttg attttcaagc aacaaactta
    52441 caattttttt gattttccga aacgaattaa accgatggca tatgcaatcg tctaaagttt
    52501 gtattttggt aaatttcatg tttttgtcga aaaaatattg cagtgtactt taaaaatcaa
    52561 ttgttatggg gaaatttttt attcttgtga tactcttgct tgcattgatg ccggctgagg
    52621 gttttacgca gtgcggaact actgatcaca acggagctga ctggattata acctcttcgg
    52681 atacattggg tggaacgcat atcaatattg gtaaattcga ggttgctatt ggtatttgtg
    52741 tttctgtcaa tccggcatgt aagttttttg cagtcgaagc tgacaccatt ttgatttatg
    52801 gcagcatcga cggtgatgcc aaaggtgatg ccggtggaaa tggcggaact ggtggtttgt
    52861 attgcaatgg tagtggtgtt cccggttccg gaggccatgc aggtttggcc ggatatggga
    52921 cagggggcgg tattccgggt gctgctgcgg gaaccggtgg aactattgaa caaatctgtg
    52981 gtggcttttt gtgtgccgga aaccaggatg gattcaatgg tggtggtggt ggcgccggcg
    53041 gcggctcagg tggttcttat ggagcttctg gtggtgccgg cgggtatggt gcttatggca
    53101 gcggtttcac cggtgctgaa ggtggagatt atggctcagg tggtacaacg tcttctgcat
    53161 atggaacggc tgatggctac gatattgcat ggggatcagg tggcgctggt ggtggcggcg
    53221 gcggcggtgg ctgggctacg ggttctgccg gcggcaatgg tggccggggt ggtggcatgg
    53281 tatcgttgaa ggccgctcac aaacttatta tgtcggggaa tatcagttgc aatggttctg
    53341 atggtggcaa tggaggcaat ggtggaggcg aatccgatga taatggtttt gattgttcta
    53401 ctactggata caatgcctgt ggagtttgtt cggaatctgt ttatgatgct gcaggtggtg
    53461 cgggtggcgc tgcgggtggc ggatcaggcg gtggaattta tcttgaaagc aatggcactg
    53521 caaccattac cggaaccatc accgcgattg gtggtgcggg tggtacggct ggcatacctt
    53581 caagtagcat gggcacttgt tacgacaatg cccggggcgg cggtggtggc agcggtggca
    53641 gagtcaaaat cctgctgaat ccctgtatta caaatcttat ttcgccggtt tgcaatattt
    53701 ctggcggcac aggcggcaat ggtgtcgtta ccggatatgt tggtacgtca ggtactttcg
    53761 aagatgattt gattgctcag gaatatattg cacttgctgg tggctctatt gctgatatcg
    53821 atacaacatt ttgtgatttt ggcgatgtgc cgcttatttc atctgttgct gcggcttccg
    53881 gtggcatggg aacctataat tatcagtggc aatacagcac aactgacagt ctttccggtt
    53941 ttacaaatta tcccgggcag accagtctga ccatggatcc atctttaatt tcggtgacta
    54001 cctggttccg tcgtaaggtt agttcgggca gttgtattga atacagcaat gttattaaag
    54061 ccgaagttgt tgattgttca gggtttgaag ataattccgg gctccttttc agtgtctatc
    54121 ctgtgccgaa tgccggagcg ttttctgttc agcttccgtt ggttatgcag aaacgggcag
    54181 aaggttatat ttataattcc agaggagaac tggtaagcga attcagcatt acagccggag
    54241 aacaaaaaac cgatattcag ctcaatgtgg caccaggact ttatcttata gttttatata
    54301 cagatcagaa tattggattg aaaaaaatca caatcgagta gtgttgtcac aataaaaaag
    54361 ctgccgcaat aattttgcga cagctttttt tatttattat gggtttttta caacaaatcg
    54421 ttggccagat tggccagttc gcttcgttct cctttttcga gttttacatg agcataaagc
    54481 gcatggtgtt taattttgtc aatcagatag cttagtccgt tgctttgtga atcgaggtag
    54541 ggcgtgtcaa tctgatgaat gtcgccggtg aatatgattt tggttccttc gccggcacgt
    54601 gtaataattg ttttaacttc atgcggagta agattctggg cttcatcgac aatgaagcag
    54661 atattgctca gactgcgtcc gcggatatat gccagtgggg tgatgaccag tttttctttt
    54721 tccagaagtt cgtcaatttg ggtggcttct ttgtcgccgt attggttttt tataaatttc
    54781 aggttgtcga aaagtggctc catataaggg tttagcttac ttttgatatc gccgggcaaa
    54841 aaacctatat ctttattgct gagcggaaca atggggcgag cgagatagac ctgtttgaaa
    54901 agttttttct gttccaatgc tccggccagc gccaatagcg tttttccggt accggcaaca
    54961 ccttgcaggg tcaccagttt tatatcaggt gctgtgatgg catgcaaagc aaacacttgt
    55021 tcagcgtttc ggggcataat gcggtaagct ggcttcttct caagcttttc aatattccct
    55081 gttattggat tataatatgc gagaactgaa gtgctggtat ttcgcaaaat gaaataatga
    55141 ttcggaatgg gttttttaat gccaacatcg ccgggtaaac aaaatccatt ctggtaaatt
    55201 gaatcaatga tatccttggt tagtccttcc accagcgtca tcccggtata aagtccttca
    55261 acatctttca ctttgccggt ttcaaagtct tcggcaataa tgttcaatgc tttggctttc
    55321 aaccgcagat tgatgtcttt tgaaacaaga atgactttgc ggtctttgta tttctgcgat
    55381 agctttattg caacattaat gatccggtga tcgactttgt cgctgtgata caactttgtt
    55441 gcatccggat tgctggtttc atccataata acagtgaaca tgcctttcgc ccgttcaatt
    55501 ttaatccagt ccttgatggg ctgattgtcc gacattttat caagaatgcg aatgaattca
    55561 cgggcttcgt aattcttggt gtcattccct tttttaaaag tatccagttc ttcaagtacc
    55621 gatataggca tcgcaacgtc gttgtcttca aagctgtaaa tcgcgctgtg attatacaga
    55681 atgaccgatg tatcaagaac gaaaatcttt ttttgttttt gggttgtttt tgccatacta
    55741 atttgatttt ttacaaataa aacattttat ttttgtcagt attgcaccat tcggtattac
    55801 tttttaacaa ccgatggttt atttgcttgt attgagggat ttgctattat tgaagtcaag
    55861 ttttcgtttt ccattgtcaa taaaacatat acttttgcat agcgattaaa atgtgatgaa
    55921 aaaaatcaag gtggggataa acggatatgg gcggatcggg aaaatggttt tccgtattct
    55981 gttgaatcat cctgaaattg aaatagccgg gatcaatgat cccatgcccg aaaattcttt
    56041 gctgtattta atgaaatacg acactgtttt cggaatattg aatgaaacta ttactgctgg
    56101 cgatgattct gtaatggttg gtaaacgcca tattccggtc agccgcgttg tttcgcctgc
    56161 agaaattcca tggaaaaagt ggggtgttga ggtggttatt gattccagcg gacgcaataa
    56221 agaccgtgaa tcgttgcagc aacacatcgt tgcaggtgct caaaaagtta ttctgacttg
    56281 ccctgctgtt tctccggttg ataaaactgt tattattggt gtgaatgagc atacgttggc
    56341 cgccggtgac catatagttt ccaatgcgtc atgcacagcc aattgtgtgg ctcctttgct
    56401 gaaaattatc agtgatagtt ttggaataga aaccggtttt ctcaatacag ttcatcctta
    56461 caccaataat cagcaattga ttgattcgcc gcacaaagac ctgcgaaggg gtcgcaatgc
    56521 aggggtcaat atcattccca cttcgtcaac ggccattaaa tgtgttctcg aagttttgcc
    56581 tgaattaggc gatcgtttca gtggaattgc tacacgcgtt cccgttcctg atggtgctct
    56641 taccgaactg gtgctgcgtc tcaagtcgat tgttaccatc actgagctga atattgcagt
    56701 gaagcacagc agcaaaacat cgatgaaagg aattgtcagt tacatcaacg atccggttgt
    56761 ttcgagcgat attatcggaa atccacattc atgcgttttt gattcactgg caaccaaggt
    56821 tatcaatggc gatactgtgc aacttttgtc gtggtatgac aatgagtttg gttattcaaa
    56881 tcgtgtcgtt gatttgattg gattaatggc gaaattcatc aggtaaatat gtttattctt
    56941 ttttcatata aaacatgctg taagcatctt caattcaggt aatcatggat aaaaagtttt
    57001 attccaaagc gctggaagtc aatcttaatc aaacaaaaca gggatttata gacttgcctg
    57061 ccgatcatag atggctcatt tctttaagcg aaaagcactg gggaataaat aaatatacta
    57121 ccgaattttt taacgaatta caccatcgtt atcccaatta tgagcatctg agtaatgaac
    57181 tccgcaaaat tgcattgggc gatttctggc tctattcagg tcataatgaa tcattgcggg
    57241 cactcggcac tatcaattct gttctttctg aattgatggt gaaacctgtt tccggaaaaa
    57301 tacttgaaca gcttgttcag acgcgtgttg atttttgtgc ttctgttgct gctttgcctg
    57361 aacccgatgc tggcttgata cgagagtcga ttcaaagtct tcaaaatctg ctttcggatc
    57421 atgttgaaat tgttttatcg aattcagttt atattcgtca gaaatggaac ttgcttgctg
    57481 atttgaaagt ggtgagtaaa gagtattttg attttctgaa agttctttgt cgcagaaatc
    57541 tggattactg gcatgtaact atgaaaatgg atcagtggtt gaatgctcgc cgaaactcat
    57601 tttcggaatt cagcaatgat gttttgagtg aatggggaag tactgcttat gaaaattaca
    57661 gcaagcgcat tgatgccgca aagtcggtgc aggatttaag cgataatgtt cccttgtatt
    57721 ctgattttgc aaaccatttt cgccggctgg ccgatcggct tgaaacgccg caggatcgtt
    57781 tccagtttat tgtgtttttg ttaagcactc ctggaatgag ccatctgagc cattttctgt
    57841 tggttgatct gaatgtggtg cttcgaacta tttttgataa tcccgatgat gaacagatga
    57901 ttccgtttct cactgaaatt ttcagtctgt ttggcgaaat catggataac aatccgtcca
    57961 ctgttcttga ttgtataaaa accatcgggc tgaaattgtc atcggtcgat aacccgaaat
    58021 tgttgaaaat ctttcagcga aaatgtcttg aattgggttt tatacgaccc ggagggcagt
    58081 atatgacttc ggagtggagg atgaatatta atccgtatca tgtgaagaat atcagagtct
    58141 ggcttgaact atttgagtct gctcctgaaa aattccagac tttgctggct gggttaacgg
    58201 ccaatctgaa actgggtggt atttttattt ttgataccga tctgtttcag aaagatgttt
    58261 ctcaattgtt gaattctgat ataaaaagca catttaaact atcgaaacag ttgtgccgga
    58321 ttttcccggt gtattttaat gaaatcggcg ctgagggcga tttacgcgat gttacaactc
    58381 agatggatga aatcagtcac cgacaggacc ggcttattca ttttttaaga aagcagattc
    58441 acatcgaaag caacaacacc catattgcgc ttactgttaa tattttccgc ttctggattc
    58501 ataaagataa aacccagctc agggattttc ttcctgaaga tgtgtatctg gcagttgagc
    58561 ccgaaggcca gtttgtcaaa catcttcatg ccatgattat ggaaatttcg gaacaacata
    58621 ataccgatcc ctatcagctg attcatcagg atccggagtt gttgtcgcaa tggattgatg
    58681 catgtggcga ttttccggtc aatgaaaaga agcggttgaa gtttattagt cgcttgaatt
    58741 tcatccttga agaaaaatat tcgttcaata ctgtagatat ttgtaagtac ctccgtcgat
    58801 tcaattttct ggcttcaacc gatatcgata ttctcgaaga tttgttgcag aatggtcgta
    58861 aagagcaggc cgtgaaacag atttataagt tcatggagca gttgaatggc atcatactta
    58921 gtgataaaaa atcggagggt tgggaaaata tttattataa gcggcatgtt gcatttggta
    58981 ttccatccat gtatggcgag tatcacgaac gtcggtttga atcattggga ttgatattca
    59041 aactggaaag actcgcagct gagcttactg aagaattgcg ccaggatctg aatttgcatg
    59101 tgattacagc caattcctta cacaaaattt caaagattct tgaactttac cgacagggaa
    59161 tcaagcttga tggaatatcg ataaaaggtt ttgatctgaa tctgaaaatg tttattttta
    59221 gtttacagtc acagagtttc agtttgcagc aacatatgaa tctgttcagg tttatgagtg
    59281 aaagcatcag ggaaattatt acccgttatt tcttcaggcc ctacgatgaa ccccttaaaa
    59341 ttgtcattcc gcagattttc gattgcaacg aactgaacga atccaagcgg atgcagttta
    59401 ttcatgccag gtctgaggaa ttttaccggg gcatgctgtc atcggcattt ttactacaaa
    59461 gtctcgatgg ttttgttgca gaagtgagcg atgctatgca taaactggca gataataatc
    59521 cgccgcagat ggttcgcgac atcatgtcgc tcaacactga tatgatgttg agtccctttt
    59581 attcgcgcac accagccatg gacaatcagg tttttcttgg atcaaaagcg tattttctga
    59641 aaacccttta tttaaatggt tttccggtgc cgcctggttt tgtcattaca acagaggttt
    59701 tcagaagaat tgatacaata ctgcaacacg atgctttaac ggcagctgta gataatctta
    59761 ttttgcaaaa tgtgcgcaaa ctggagaaaa taaccgggaa aaaattcgga agcaaatcca
    59821 atccgctttt gctttcggtg cgttccggta cggccatttc gatgcccggc gccatgaata
    59881 cttttttgaa tgtaggcatt aatgaagaaa ttgcagaagg gttgagtaag caggaaaatt
    59941 atgcatggac ttcgtgggat tgctatcgca gatttttaca aacatggggg atgtcttacg
    60001 gtatcgaccg cgatgtcttt gatcagatta tgattgattt taaaacgcgc taccatgttc
    60061 agcaaaaggt tcagtttaaa ccagagcaaa tgcgtgaaat ggcgatttgt tataaagagc
    60121 tgattctgaa acagggtatt cccatcgaag aagatcccgt aaaacaattg aagcaaacta
    60181 ttttaaatgt gtttaattct tggtcgtctc ccaaggcggt ggtttatcgc aaaattcttc
    60241 agattgccga tgagtgggga actgcggtca ttgttcagaa aatggttttg ggaaatctga
    60301 attatacttc cggaacgggt gtgctgttta ctcatgcacc gcatgtgagc aagccaggta
    60361 tttttctttt tggcgattat tcgctggtta gtcagggcga agatattgtg ggtggattgg
    60421 tgaatccgcg tgcgctttct tcgcgacagc ccaacgatga taaccctgca ttatcccttc
    60481 aggtcttgtt tcccgatatt tttgaacgtt tgttgaaatt ggctactcac atgatcgaag
    60541 ataatgggtt tagtcatcag gaaattgaat ttactttcga gtcacagaat ccggatgatt
    60601 tgtatatcct tcagactcgt gatcaggacc tcacttcgca atcgaaacca gatgtgtttt
    60661 tcgagcccgt tgctgaggaa catttgcttg gccggggaat tggtattggt ggtggggcga
    60721 tgaatggtat tgttgttttt gacgataacg atattcgtca tttcagaaaa tcaaggccgg
    60781 attgcaacct cattcttgta cgacccgata cggttcccga agatatcgga cttatttttg
    60841 aaaccaatgg attggttaca gcacgtggtg gagccacttc gcatgctgct gtaactgctg
    60901 tccggcttgg aaaaatttgt gtggtgaatg tggctagtct tgttgtcaat gacagaaaaa
    60961 aacagtttag tgtcggatct gttgttgtta gtgctggtga tgaaatcgct atcgatggca
    61021 ccattggttg tgtctataaa ggcaatttca aacttgatgt tttcaaaaat cagtctttgt
    61081 tgaattgaaa tttaaaagta aataattatg gaaatcagtg taaagaacaa aaggttactc
    61141 ggcattaaca gtctgggtcg aattgggaaa ctcacccttt ggaatcagct tgccaccagg
    61201 aattttgacg gtattgttgt aaatgttggt cgcgaagtag gcaagaccac cgatgattta
    61261 ttgcaggtgc ttgaaactga tacaacctat ggtcagttgg gaaaattttt gcatggtgtt
    61321 gccggaaaac cgcttgtttc aaaagttgtc gatgaagcaa atatgctttt tgaaattgat
    61381 ggaatgcctg tgaaagtttt acgtagcgag cgtaatccat caggtattcc gtggcgtgac
    61441 gaaggtgtgc aacttgtaat tgactgcacc ggacaatttc tcgatcctgt tgttccgccc
    61501 gatgcaccca agggcagttt gcggggtcat ttggttggtg gcgcgcgtaa agttattgcc
    61561 agcgcgcctt tcaaaatcaa ggacaaatcg gcccgtatgc ccgaagatgc actgatgatg
    61621 gtgtttgggg ttaaccatct gaattataat ccatcggtac atcatgtgat atctgctgca
    61681 agctgcacca caaccggcct ttcgcatatg atgaaaccat tgctcgagga tgctgaaact
    61741 tcaaaaattc ttacagcttc catgtcaaca gtgcattctg cgactaatac ccagtcggtg
    61801 ctcgatgctg ttccaaaagc agctgccagc gatttgcgca aaagtcgctc ggtattcaat
    61861 aacattattc tttcaacgac cggtgcagct aaagcgcttg aatccattct gccggatatt
    61921 caaaaaattg gctttatggc cgattcagtg cgaattccta caacaacagt gtcgttgatt
    61981 acactcaata ttacttttaa ttcaggtctc gatgaaagcg gaaatccact gattaaccga
    62041 aattacatca accggattta caaaaatgca gcaactggtg cacaaaaaga tttgttgatt
    62101 ttcagcgaac ggcaaaacgt gtcggccgat atgatgggtt ttcgcgctgc agctgtgata
    62161 gaaggatgtg aaacgcatac cagaacgggt tttgtgaatt tgcccgcttc tatgttcgag
    62221 gcctatggaa tgaaaaatgc cagcgatatc aatatgcccg taacacatgc aaaaatcttt
    62281 ggttggtacg acaacgaatt tggaagttat gtaaccatgc tttcaaagct ggccgccttt
    62341 attaattcaa aattgtaaaa gggtttttta aactgtaata atggtatgac ggaatcagca
    62401 tggttgcgtt ataccatttt ccctttcagg gtggcagctg tgcagtctgt gataattgta
    62461 tttacaagcg tgccgggaag aatattttcc gcatcaaaaa caacgacttt gttttgtccg
    62521 gtgcggccaa acatatgttt gtccgagcgt tttgaaaatc cttccaccag cacttcaaag
    62581 gtctttccaa tgtcgcgctg attatggtgt agcgaaagct cctgttgcag attaatgatt
    62641 tcggtgagtc gttgcgattt ggttttttcc ggaacgtcgt ctggtatttt tgctgcagat
    62701 gttccttcgc gacgactgta tttgaacatg aatgcataat aataatcggc ggctttcatc
    62761 atgttcagtg tggcctgatg gtcttcatcg gtttcaccgc aaaatcctgt tattatgtcg
    62821 gtgctgatag caatgccggg catatattgg cgcaaaagtt caattttttt cagatagtct
    62881 gcagccgtat atttccggtt catgcgttga agaacggcat cagatcccga ttgtacagga
    62941 aggtgaatcg atttgcaaat gttatcgtgc ttagccatta cttttacaag attttcggac
    63001 aaatcttttg gatgacttgt tgcaaagcgc actcgcatag aaggaaattc cgaggccact
    63061 ttatcgagga gcttcggaaa atggtattct attgattttt cagtgaaatg ataagagttt
    63121 acattttgac caaggagggt gatttctctg aatttttttt catgcaataa ttctgtttca
    63181 tgaagaatgg actgaaccgg ccggcttcgt tcgcggccgc gtgtatatgg tacgacacag
    63241 tagctgcaaa aattgttgca gccgcgcata atggatacaa aggcagtgat ttctccggtc
    63301 tggtagtgct ctggatcaat atcttcgtat gtttcggtgg tgcttaaaat cacgtccgag
    63361 gcctttttat gcgtttctgt ttcccgcaat aaatcaggaa gttttttata actgtcggga
    63421 cctgccagca aatcaatgcc cggtagcttt tcaaaaatac tttcagccat gcgttcagcc
    63481 atacagccaa tgattccgat tcgtaatcct ggttttttct ttttcagtgt ttgcagctca
    63541 cgtccccgtg caagaacttt tgtttcagca ttttcgcgta tgctgcacgt gttcaccaga
    63601 atcagatcgg cttctgttgc gtcgttgcag atatcatagc cctcttcacc aagaatcgat
    63661 gccactacct cgctgtcggc cacattcatc tggcacccat atgtttcgat gtatagtttt
    63721 ttgttttttt ctgtcattgg aatgcaaagt tactaatctg aaatattcct gataacttaa
    63781 tttctccctg ttgtttttat acctttaatt gttatgatgc aattaattga taatcaattg
    63841 ataagtggat tcgatgaaaa attttattga catatcccat ttttattcat ttaatttgtg
    63901 gaaaatttga gagaatgggg aagaatttag ttatcgttga gtcaccggca aaagccaaaa
    63961 ccattgagaa atttctgggc aaggattatc aggtattatc gagctatggt catgtgcgtg
    64021 atctgcagaa agaagacctg agtattgatg tgaataatgg atttaaacca ttgtacatca
    64081 ttttgccgga taagcagaaa atcatcagtg agttgaaaaa actgagcaaa caggccgaaa
    64141 ccacatggct tgcaactgat gaagaccgtg aaggagaagc catagcatgg catcttgccg
    64201 aagcgttgaa tctggatgtt aaaaagacca agcgcatcgt ttttcacgaa atcacaaaaa
    64261 cggctattct tgaggccatt gaaaatccca gaaatctcga tcttaatctt gtcgatgctc
    64321 agcaggcacg gcgcgtgctt gaccgtctgg tgggttacag actttcgcca ctgctttgga
    64381 aaaaagtaaa accagctttg tcggcaggcc gggttcagtc ggttgctgtc cggctgattg
    64441 ttgaacgtga agatgaaatc aatcagttta cgagtacatc ggaatttcgt gtcaatggtg
    64501 tttttgaagc aggtaaggat tcagtaaatg ctgagttgaa tgagcgtttt gaaaatgagc
    64561 gcgattcgat gatgtttctc gataaaatca aggacagtat tttttctata ggaaaaattg
    64621 aagtgaagcc gggtacacga aatccggccc cgccttttac cacttctacg ctgcagcagg
    64681 aagctgccag gaaactggga ttttcggttt cgcaaaccat gaccatagca cagcaattgt
    64741 atgaggcggg gttaattact tatatgcgta ccgatagtgt aaatctttcg aagcttgcaa
    64801 tcggtagcat taaaaacgag atagagaaat cctttggtaa agagtattcc aagccacgac
    64861 agtttgcaac aaaaagcaaa ggtgcgcagg aagctcacga agccattcgc ccgacctatg
    64921 ccgaaaacag ggagatagaa ggtcctgcag ctcaaaagaa attatatgat ctgatctgga
    64981 aacgtacagt ggcttcgcag atgggggaag caaaaattga acgtacctct gttcagattg
    65041 atatttcagg tgtgccgcaa tattttgttg ctcggggtga ggtgattgtt tttgatgggt
    65101 ttttaaaggt ttatgaggaa tcgcgcgatg acgatgcatc ggatgatgat aaaggtcaat
    65161 tgccaaaact tgccgaaggg cagaaacttt cacagcagaa aattattgct gaacaacgtt
    65221 tttcgcagca accacccaga tacagcgaag catcgcttgt gaaaaaactc gaagatcttg
    65281 gaattggtcg cccaagtact tatgctccga ctatttctac tattcagcgc agagaatatg
    65341 tgatcaaaga aagccggcag ccggttgaaa gaaaatatat ctctcttctc ttggcaaagg
    65401 gtgctatcaa aagaacggtt gaaaccgaaa agtttggaag tgaaaaaaat aaattgttcc
    65461 caaccgatct tgggatcatt gttacaaaat ttttggtgga acatttcgaa aatattgttg
    65521 attataattt cacagctacc attgaaaagg attttgacga aattgccgat ggaaagaaaa
    65581 agtggaatgc tttgattgcc aatttttaca aggattttga tccacaggta atcaatattg
    65641 acaaaacaac cggcaaatac catggcgaac gtttgttggg tatcgatccg gaatcaaaga
    65701 aaaatgtgta tgtaaaactt ggcccatttg gccccatggc tcagattggt gaatcaaccg
    65761 atgaggaaaa accgcgcttt gccggcttgc ttcgtggtca gaccatcgat ggggtaacgc
    65821 tcgaagaagc tcttttgctt ttcagattcc ccaaaatagt tggtgaacac gaaggcaaag
    65881 aggttgttgt ttctgttgga cgctttggac cttatgtccg tttcgacggt aagttctatt
    65941 ctattccgcg cgatatcaat ccgactcagg ttaatctggc ctctgctctc gaaatcattg
    66001 ccaacaagca ggagaaagac agaaacagtg tgctcaaaat ctttaaagaa gagccggaat
    66061 tgcaaattgt caaaggtcgc tttggcccat atatcaaatt taaaggaaaa aactatcctt
    66121 tgggcaaagg tcgcaaatat gatgcattga ctgtgaccga aatccttgaa attgttaagg
    66181 agcatcctga aaaacccagc cggaagcaga aaacggttaa agctgccaaa acaaagaaaa
    66241 cggctgccac agaaaaggca gaacccaaaa agaaagcgac agctaaaacc acaaaagcaa
    66301 aaacagcaac aaaaaaaacc actgcgaaaa aaacatctgt ttcaaaatct aaaaagaaat
    66361 agtccatgaa cgagctgcag ttgtattttg ataaggttga cactgcgctg tttcccgaaa
    66421 atttcatgcc ggggcagatt ggtgcagcgg tctcgtttaa tacgggtgat aatttcccgg
    66481 atactgaaaa tattcagatt gccttattgg gtgttcctga aaaccgcaat tcgcaaagtt
    66541 cccgcgatgc ttcttttgcc gctgatgcca tcagacctta tttttataca cttcagagtc
    66601 attttcccaa tgttgctatt gccgatcttg gcaatctgaa aaccggcatg tcggtcgatg
    66661 atacctatgc tgctgttgct gaagtagtga atatcttgtt aaaagataat attttcccgt
    66721 ttattcttgg tggaagtcag gatcttacct atgccaatta tctttcatat gaaaaaagag
    66781 gacggattat caatattctg tctgtcgatt cgcgttttga tattggcaag tccgatgaga
    66841 ccttccataa tgaatcatgg ctaaataaaa tcattttcag agatccgaat ttccttttta
    66901 attattccaa tctgggttat cagacctatt tagttgaaca agaggcaatt gctttgatga
    66961 aaaagctttt ttttgatagc acccgtcttg gtcaggcaaa ggccgatatg actgaaactg
    67021 agccattgat aagaaatgcc gatctgcttt cgtttgatat gtcggctatt cggatgagtg
    67081 attgtccggc tgcttcattt ccatcgccca atggtttcaa tggcgaagat gcctgtcaga
    67141 ttatgcgcta tgccgggttg agtgaaaagc tttcttcggc cggcattttc gaattatgtc
    67201 ccgccaatga tcgcgacggc atgtcggcgc atttggctgc tcaaatgttg tggtattttc
    67261 tcgatggctt tgctggccgt aaaggcgatg acccgcatat gaacaaatcg gcactgatga
    67321 aatacattgt ttcgatgcat gaaacacaat ccgatatttc attctatcga agcccgctca
    67381 gcgaccgctg gtggatggag gtgccatgtc ccaacaatct tttttcaaaa ttcgaaagac
    67441 attatatggt gccgtgtagt tacagagatt atgaagaagc catgcaggaa aatctgcccg
    67501 accgatggtg gcagactttc cagaaattta tgtagaaatg gactggtgtg ttaaatttta
    67561 cttcagcttt tttgcaatct gaaaaatttg gcataagcta tcggatatgg ctctattaaa
    67621 cactttcatg agtgccaatg cttgtaattc cttttctcag gcatttcccc gaatcgttga
    67681 tctctcatgc tgatttaatg catgtttgat tgcatattcc gaagaaaatg aaaaatttat
    67741 ttcttaaatg aaaccaaaaa aaaattcaac agtataaaat atccgtgaat cttttggagt
    67801 tattttcaag aaagaacgaa aacttttcaa aaaatatgaa cattttgttg gcaatatcag
    67861 aaaatcttcg ttactttatg cactttttta aggagagtca atctaaaatt ttgattatga
    67921 ggaaaaatgt acttttcctg tttttggtaa ttttggcttc tggtgcggta aacgcgcagc
    67981 aggatgctca atttactcag aacatgttta atcacatggc catcaatcct ggctatgccg
    68041 gcagccatga tgctatttgt gctacagctc ttttccgtca gcaatggatg ggatttactg
    68101 aaacagtgca cgataacaca aacaaagtgg ctccacaaac catgttattc tccattcaca
    68161 gcaatgttga tgcgatccgc ggtggactgg gtctggtggt ttacaaggat aaacttggtt
    68221 atgaagacaa tattggtgtg aaattcggct atgcctatcg tatgcctttg cagggtggta
    68281 cattgggaat tggccttatg gctggtttcc tgaataagaa aatggatttt tcccaattca
    68341 atcctcagac tgccggcgat ccgctgcttg caagtcaggc agtagagagc gatatggtat
    68401 ttgacctttc tttcggagct tattacaaca aacctggtgt tttttatgcc ggcttatcat
    68461 ccagtcagct tatgcagagt cagactgatc ttgcctcgac ggttacaaca ctgggtagcc
    68521 cgcagcttaa acgacattat tatcttattg gcggttatca atatcagatt ccaagcaatc
    68581 ctcagtttga actcagacct tccttgctca taaaatctga ctttgcaagt acccaattcg
    68641 atattaatgc tctgatgtat tataacagtc agttctgggg cggtctgagc tatcgtaccc
    68701 aggatgccat cgccattttg cttggagcgc agccatttat caattccgga aacagaagtc
    68761 ttgaaacact cggaattggt tatagctacg atgttaccac ttctgccatg ggcggaggag
    68821 gtcggagcag cggtagtcat gagattctgc tgaactattg ttttaaaatc gtcataacaa
    68881 ttccgccaga cagctacaga aatgtgcgtt tcctctaaaa aaatcagttt gtgaaacttc
    68941 gtcgataaaa actgcgttaa aaagagatag gttttattaa atttgcaccc atgaagtcca
    69001 acctccaatc aaatatgaag tacctgacag ctttagtgtt gcttgttctg gtcatcacca
    69061 gctgcgacac atctgatcgt ggcaacctgg tcggtgtcca ggatcgcgaa ccctggtatc
    69121 aacccgatcc ttacgggatg ctgttcattc ccatgggcag ttatgtgatg ggtcagaacg
    69181 accaggatgt accgtatagt caactgcaga ctgctaaaac agtgtctgtt cagtcgtttt
    69241 atatggacga aacagaaatt accaacaacg agtatcgcca gtttgtgtat tgggtgcgtg
    69301 actcaattgc ccgtcagtta cttggcgagc agtttgagga ttacctgatt atgaccgatg
    69361 aatacggtca ggatttggaa ccgccttatc tcaattggaa aaccagactg aggtatgacg
    69421 atgatgatga aaccgttcgt gaaattcttt cgcagatgta tatgccggaa cacgagcaat
    69481 tttatcgtcg taaggaattt gatgcacgta agctgaattt ccaatattat tggattgatt
    69541 ttaaagctgc tgcacggaag gacttccgta atccactcac ggacgctcag gtgaagtatg
    69601 aagaagacgc tgcatcactg cgcaacagac ctcagggtta caacgaccgt tctgtgttta
    69661 ttaaaagtga tattattaat gtatatcccg atactttgtg ctgggtgatg gattttacgt
    69721 attcattcaa cgaaccaatg actcagaatt atttctggca tccggcatac gatcattatc
    69781 ctgtagtcgg tgtaaactgg aagcaggccc gtgctttctc gatctggaga acccagtatc
    69841 tgcacaatta cctgagaagt attggtcagg ccatagtcaa tgacttcaga ttaccgaccg
    69901 aaagtgaatg ggaatgggct gcccgtggag gtcttgaact gagtccttat ccctggggag
    69961 gtccgtatat ccgcaactcc aatggttgtt tccttggtaa ttacaaacca ttgcgcggaa
    70021 attatccgga tgatggtgga ttccatacat tgattgtagg tcactatgct ccaaacgatt
    70081 ttggtttgta tgacatggct ggcaacgtgg ctgaatggtc aagcaatgct tttgatgaat
    70141 cagcttatca gtttgcacac gatcttaata tggattattc atacgaagcg aaagatactg
    70201 atcctgaagt tcttaaaaga aaagttattc gcggcggctc atggaaagac attggtttct
    70261 tcatgcaggt cagttcgcgt tggtatgaat tccaggatac cggaaaatca tatatcggat
    70321 ttagaaacgt tcagacatac ctgggccgca tgaagggtga tggtcctaac tcatctaacg
    70381 tttacagata aaatcgaaag ggtcaaaaaa acacaacaag tcaaattttt aaacaaaagg
    70441 ttatgggtat taaacaaatt gttgaaagta aagggtacaa aaactttatg tctaagctgt
    70501 atggctgggg cgcatctctt gtaatccttg gtgctctgtt caaaatcatg cactatccgg
    70561 gtgctggtgt gatgcttctg attggtatgg gaactgaagc tctcatcttt ttcgcttccg
    70621 cttttgaacc aatgcatgtt gaaccagact ggagtctcgt ttatcctgaa ctctggggaa
    70681 tttatcaccc tgaagaagct gaaaagcttg gatgggaacc gcaaaaatct gttgctacag
    70741 gtaaaacagt tactcaggaa ctcgacaaaa tgcttgccga tgccaaaata ggacccgagc
    70801 ttattgccag tttgggtgaa ggaatgaaaa atctgagcga aaatgccaac aaactttcag
    70861 gtgttgctga tgcagcttct gctaccgatg gttttgtaac caatctcggt gctgcggctt
    70921 ccagtgtttc aggtttgaaa gaagtttatg acaaaacagc caactcactg agtaagacgg
    70981 ttggagttaa tgatgaattt gttgaaaccg ttaaagctgc ggccaacaat gccaatgaaa
    71041 ccgctggttc attcaaggcg gttgccaatg ctatcaataa cgatgttaat gctactgaag
    71101 aatatgtgac cagtattaaa ggtgctacta ccgctgctca tgctcttgcc cagaaatata
    71161 cccaaagtgc cgaatctctt accaaatctg ctgaagctat tgatttcagc gccgttgacg
    71221 gaaaatccta tggtgatcag attcagcgta ttactaagaa cctttcagct ctgaatgcag
    71281 tatatgaact gcagctgcag ggtgcaaatt cgcaggttga aaaagccaat gccatgcagg
    71341 aaagtcttgg tgcttttgtt gataatgtca atcaaaccat gacagccctg aattcttata
    71401 aagatcaggc aaacgttctg tcgaaaaatc ttgctgccct caacactgtt tatggcaaca
    71461 tgctttctgc aatgaatgtc aacctcaata agtaatataa ggtgtattgt ctaatcattt
    71521 aaagaaaggg taatttatgg gagcaaagaa ttgtccggaa accccgcgtc agcgaatgat
    71581 ttcgatgatg tatctggtgt tgaccgctat gcttgccctc aacgtatcaa aagatatttt
    71641 gcaggcattc actgtggtga acgacagtat cgttgaaacc aaccgcattt ttaaagcgaa
    71701 ggttgatgga aactacaata tgtttaaatc tgcctatgca ttgactcctg gtaaggtaaa
    71761 ggagaattat gaaaaggcac aaactgttca gaaagaagcc gatgcacttg tggcttttat
    71821 tcagcaggtg aaatgggaaa ttatttccaa ggtggaaggt aaaacccccg aagaagtaaa
    71881 agccatggaa gaagctgcag ccaaagaagg caaaagtttt ctggccgata tcgacggaaa
    71941 agacaactat gatgttcctc accattattt ctttggcgat tccgaagatg gtggaagtgg
    72001 aaccaaagtt tttgaactga aagacaaaat catcgcgttc aagaaaaaaa tgactgattt
    72061 gcttgggcca aaatatgcca agagtgtaaa cctggggctt gaagtggaaa aggattatcc
    72121 taccgtttcg ggcgaaggca cagcaaactg gcaggttacc aatttctatc ataccattct
    72181 tgctgctgat gttgttattc tcaacaaaac aatcctcgag gtgcgtaatg ccgaagctga
    72241 cgttgttgct cagctgtaca acatggtgga tgccgaaggt ttttcttttg acaaggtaga
    72301 agccaaagtt attgcaaaaa gcaattatgt gcttaccggt agtgaatacg aagctgaaat
    72361 ttttgttgca gcttacgact caaaacagca gccaaccatt cttgttggtt ctggtgttga
    72421 ttccactacg cttgaagttt cgggaaatgt cgaaactatt gaaggtaaag atggtgttgg
    72481 ccagtacaaa gttggagccg gatctattgg tgaacagaag tttggtggtg tcattgaagt
    72541 aacctcgcgt aatggtgtga aaacaaaata tccgttcaac gcttcttact tcgttgcgca
    72601 gccatctgcc actgtttctg ccgataaaat gaatgtcttt tacattggcg ttgataaccc
    72661 tgtaactatt tcggttcctg gcgttgccaa tgaaaaggtt cgtgcaagca tttcaaatgg
    72721 ttcactcagc ccaaccggcg gtggacatta caatgtgcgt gttaccggtg gtgccgaagc
    72781 tgtcatcaac gtttctgccg atatggatgg aagttcacgt ccgatgggtt ctaccaagtt
    72841 ccgtgtgaaa cccatcccaa ctcctattcc gaaagtagcc aacaaaatct ctgggaactt
    72901 cacgaaagct gaaattcttg gatctccata tgtgttagca gttctcgaaa attttgattt
    72961 tgacttgagg tataatgtgg tatctttcaa attcacttac aagaatcctg ctggtgacct
    73021 catcgatatc cctggtcagg gatacttact gaatcaacaa atgaaaaatc ttatccagaa
    73081 ttcgcgccgt ggcgatcgtt tctgggtaga agatatcatt gctgccggtc ctaccggtaa
    73141 caaacagatt ggatctgtta caattagaat tactcaataa agcttgaatt atgaaaacgc
    73201 tgtttgtctc tgtggttgta atgcttggtt ttaccatggc atccctggct caggtatttg
    73261 ataatccacc tgacagagtc tgggacaaag aatactatcc cgaccgtgta gcggtaccct
    73321 atccgcattt gcgtaaggcc gatgttttct ttgcaaaacg aatctggcgc gttattgatt
    73381 tgcgcgaaaa aatcaatcat ccgctgtatt atcccattca acctaccaat ggacgaatca
    73441 gtcttatgag tacaattctt aaaggaattg aagaaaactc tatcagagct tacagcgcca
    73501 tcgatgatga gttcaaaact cccatgtcgc ctgaagaggt gaaaggtctt cttgttaaaa
    73561 ttgacacgct gaaggataga ttcgatccgt ttacaggtgc tcagctgcct gatacggtga
    73621 ttacaactcc tttcgatgtg gcttcggtga ctatgttccg actgaaagag gattgggttt
    73681 tcgacagaca gcgttcagtt cttgaatgcc gtattttggg aatctgtcct ttgcgcgagg
    73741 aatatgacat cgatggaaac ctcaagggta cacgtgccga tttttggatt tattttcccg
    73801 aagccagaaa tgtgcttgtt aaaaacgagg tctttaaccg tttcaacgat gctcagcgtc
    73861 tgacttatga tgactggttc ttcaaacgct tgtttggcag ctatgttatc aaggaaacaa
    73921 atgtgtatga ccgttatatc gtttcatatg caaccaacct tgacgctttg cttgaaagtg
    73981 aacgaataga aaacgacact ttcgtgtttg agcacgattt atgggaattg taacaaacag
    74041 attttgttta tcattggctg ctctcttcgg agggcagctt ttttttatac catgccccag
    74101 gtttcgcggt cgagactgcg gtattggatg gcttcggcca gatggtgatt ttcaatgtct
    74161 gcagattgct ctatatctgc aatggtgcgc gccactttta aaatccggtc gtaggcgcgt
    74221 gccgaaagtt tcagcatatc catggccttt tgaatcagtt gctcgccttc aggtttcaac
    74281 tgacagtatt ttttcattaa agctggtgtt agctgggcat tgcaggaaat ctggctatgt
    74341 tccttgtatc tttcctgttg tatcattctg gttgcgatga cccgctgacg gactgcctgc
    74401 gaagattcgc ttttcttttt gtcggtgaga ctactgaatg gtaccggtgt aacttcaata
    74461 tgaatatcaa tccggtcgag cagcggccct gaaatgcggc ttaaatagcg gcgtatggct
    74521 ccgggtgcgc aggtgcattc tttttcaggg tgattgtaga atccgcatgg acaggggttc
    74581 atggcagcaa taagcatgac cgatgccggg taatcaacag taaaacgtgc ccgcgaaatg
    74641 gttacaatac ggtcttcgag tggctgacgc attacctcaa gagcactgcg tttaaattcg
    74701 ggcagttcgt cgagaaataa aaccccgttg tgagccagac tgatttcgcc cggttgagga
    74761 taggtgcctc caccaatcag tgccacatcg ctgcatgtgt gatggggtgc gcgaaaaggt
    74821 cggtttttaa gcagtccggt gccttgcggg agtttgcctg ataccgaatg gattttggta
    74881 gtctccagtg cttcatcgag cgacattgga ggcaatatgg taggtaatcg acgggccagc
    74941 atggttttgc cacttcccgg cggtccaatc ataattgcgt tatgtccgcc agctgcagca
    75001 atttccatca cgcgttttac attttcctga cctttcacat cgctgaaatc gaccggatat
    75061 ggacttcctt tttcttcgct gattgaatat cctgaagggc agggatccat ttcggtagtg
    75121 ccttcgagaa attgaactac ctgacggata tcgtgcaggg gaaaaacctg aaggccatct
    75181 actactgagg cctcatgggc attttcggca gggacaataa ttcccttgaa attatttttg
    75241 cgggcttcga tggcggcaga aagaactcct tttaccggtg ctattgttcc atctagtgaa
    75301 agttccccaa ctatcatgat ttctgacaga cgttcgcttt ctatttggtg tgaagcagcg
    75361 agtacggcaa ttgagatggc aaggtcgtag gccgaacctt cttttttcat atctgctggc
    75421 gccagattga tgaccactct gcgtccgggg tagcggaaac cgttgtgaac aagcgcagaa
    75481 cgtattctct ctatgctttc tttcactgca ttgtcaggta agccggcaat aaaagtcttt
    75541 gttccggatt gaatatcgac ttctaccgaa acggtataag cttcgattcc ctgaatggca
    75601 cagcaaaatg ttttaacaag catatgaatt attctgagat cggttaacaa aaataatcaa
    75661 atactttgtt gcttatctga caaaatggaa ttatttttgt agaaaacagc gaaaactcta
    75721 tgaaacgctt tttcaatatt tttttgattg ccctgatgag cgcaacttta ctggtttctt
    75781 gtcagccaga cgaagatgat gatgatattt cgaatgatcc gcgcagcgat tatcaaggtg
    75841 tatgggtgtg ttccgaaatt ggcggacagt catacactgt gaatatttca attgatacgg
    75901 ccacacaatc gcagattaca ttgttcaatt ttcatcatca gggatttgaa gaaaaaatca
    75961 atgcaattgt atccggaact tcgctgacca tcaaccccca gaccatgtgt atgggaaccc
    76021 tcactgttga aggaaccgca accatgcaga gtaataaaac gaatatgaat ttttattaca
    76081 cggtaaacaa cggcgttgat atggacacta ttcaggccaa ttataccaaa cagtcgtcct
    76141 gaagggacta aacggtctta ttgaataaac catttcgtac ctgacggcac gggttggcgt
    76201 tcttttttga gctaccaata ttttgtccct atgggacaat tgcagctatg tctgctgttt
    76261 cccttgggct ccgaattccg ggacaaatcc gcgccgctcc taaagaactg aaataacacc
    76321 aaagcgtttt tttaatcggt gcaatcattt ttggaagact ccggaggagt cacatatctg
    76381 tagcccataa atacacaaaa tcacgactcc ggaggagtcg catataaaga gctgcgtcct
    76441 ctgacaatgc ctgtatatat gcgcttttgc agaggtagtg gttgttaagc cggtttgaaa
    76501 atactttact gattatgctg ttattttgtg aaaaatgaca gacgtaaaaa agatgatcag
    76561 atttgtttat tgatcttttt ctaacattgt ttccagtata atcagactat cgagcgcctg
    76621 attgcttatt tgcttcagtt tttccggttg gtgcgcccag tatctgtagg aacgtgttat
    76681 ttttgaagtg tcgcaatgat atttttcaag cattaactga tagtaatggg tggcatgcac
    76741 cgatacatct ttgcccgttc gctcaatttg ccggatggat gcttctgtga gataaaaatc
    76801 aataagcagt gtcttcatgg agtcttgtgt aaacactgaa tcagggctga tttcagatgt
    76861 ttcttttttt gaggaacaag ctacagcaag caataaaaca aataatatga agatcagctt
    76921 tttgtacatg ctgcaaagat acaaaagagt agtgagttaa gccctgaaat cctgtgagca
    76981 acccaagcct caggatcaag ttaaacaact gttcggaaaa cataacttct ttttactact
    77041 aaagtagtac tttccattaa attggtggta gaaccacctt tttcaggaac tgacaactga
    77101 caaattcata acaactttcg ccttattcgt attgtcattg ttacttttct ttttacatcg
    77161 atgactgtgt aatagattgt catagcttgc cacgatgcaa gtggttccac gtgattttat
    77221 gtgagggtgt tatattttcg caacttttat gcgatatcag acagggataa tcctttgaaa
    77281 cgttctttca gagccgtttt taaacatgcg ttttcggctt ttgaaagagt tggatcgtct
    77341 gtgcagattt gttcagcagt ttgtcttgca aaggtgatga tatgatcatc acgaacgaca
    77401 tcgaggtgaa taaattcaaa ttctccgctt tgccgggttc ccatcaggtc gccgggaccg
    77461 cgtagtttta gatcggcttc ggcaatttta aaaccatcgg ttgtttctat catggctttc
    77521 agtcttgttt tggcgtcgtt gctcaggttt ttacctccaa ttaaaatgca ataggattgg
    77581 aattttccgc gaccaactct tccgcgcaac tgatgcaatt gcgacagtcc aaagcgttct
    77641 gcattttcaa tgattattac cgatgcattt gggacatcaa cgccaacttc gatgaccgtt
    77701 gttgagacaa gaatgtttgt caatccgcgg acaaagcgtt tcattacaaa atctttttcg
    77761 tcctgaggca tgcgtccatg cataatccca acctcgtatt ccggcatggg gaagtcgcgg
    77821 ataatacctt catagccatt ttcgagagct acaagatcca gcttttccga ttcttttatc
    77881 agtgggtaaa caatataaat ctgccgacca ttttttattt gttttcgcat catttcgatt
    77941 acataatagc gctggtcatc gtagtagaat ttagtaataa tttcttttcg gccgggtggc
    78001 aattcatcaa taatactgac atccaggtcg ccataaaagg tgagtgcgag tgtacgagga
    78061 ataggtgttg cagtcataac cagcgtgtga accatttcag aacctttgga gtggagcttt
    78121 gcgcgctgca tgaccccaaa gcggtgttgt tcgtcaataa tggcgagtcc caggtttgaa
    78181 aattcaacag gatcctcaat cagagcatgc gttccgacaa caatatgaat agacccatcg
    78241 gccagcgatt tcagaatttc cttacgtcct ttgttttttg tgctgccggt gagcaattca
    78301 acgcgcagac ccaaatcagt tatccatttc gaaatggttg caaaatgttg ctgagccaat
    78361 atttcagtag gcgccatcag gcaggcctga tatccgttgt cgatggccag cagggctgcc
    78421 atcatggcaa cgacggtttt cccgctgcca acatcgccct gcaacaatct gttcatgcgg
    78481 tatccactac caaggtcggc tctgatttcg cgcgtaactc ttttctgagc cgctgtcaga
    78541 gcgaaactga gcgattgatt gtaaaaagca ttgaaatatt ttccaacttc tttgaatata
    78601 attccagttg aggttttctt tcggttgtcg gcgtttattt tgtgccggag ctgcatcaac
    78661 agtgcttctt cgaagcggaa tcttttgaga ctacggtcaa gaatgttttc atcctgtggg
    78721 atatgaatgt tttgatatgc ttcattgata ccgggcagct gaagcgattt cagcagtttt
    78781 tcgggcaacg tttcattcat ttgcccgctt gttttgttaa gtgcagcagc cacaaacatg
    78841 gatatggcct tcgagtcgat gccttttgct ttcagctttt ccgaagtctg ataaatgggt
    78901 tgaaaacgac cggaaaatgg caatcgcttg aattcatccg gtgtgaggat ctcgggatgc
    78961 gccatgtata gttgtttgct gaaaagccca acacgtccga aaatataata tcgtgttccg
    79021 gttttgtact tgtcggtaac ccatgagaaa ttctgaaacc agatgatttt aatggcggct
    79081 gtatcatcat ggaaaatggc ttctaaacgc gaatgacctg ttttacccac tttgacgaat
    79141 tcatcaaaaa aaccggatat ctgcacaaaa ttgtcatcgc ccgaaagtgt ggcaatagga
    79201 gtaatacgtg tccggtcgat gtagcggaac ggaaaataat tgagcaaatc ctgaaattca
    79261 aaaaccgata attccttgtt cagaatttca gcccgtgccg ggccgactcc tttcagatat
    79321 tcgattttat tattcagaat tgaatcagac attgagcaaa tataataaat atgcgcacat
    79381 gactttcttg taagccatga aattttgtgc tgtcggaaaa aagcaattat ctttgtgtgt
    79441 ataataaaag ttgcttcaaa cgttattgta ttgatgaaat atcccgggcg cctgttttta
    79501 atgtggtttt tttctgtgac aatgctacat gcatttgccc agaaagaaaa gcttgtggta
    79561 cagactcctt caacagatag tcttatggct gcatccatgg ccatgtacaa tggtaaaaca
    79621 gatatggccc ggtacgaggc aaatgagaaa tttatacaac ttctgaaaaa agacctcagg
    79681 gaagaaaacg cttttgatta tgtttttgat agtattcgta tcctgtctgt tatttatcct
    79741 gacgacagaa agtttaaggt cttcagttgg aatttgcctc tcagtaattt caaaaatgaa
    79801 aattatggat tgatgatggt tttttctgaa aagtcgcata attacaagct gattgaattg
    79861 cgtgatgttt ctaatgacat tattaatcct gaaaaggaaa tcttaaagaa aggattgtgg
    79921 tatggtgcgg tatattatca attgatagag aagaaatttg atggtaaaaa agtttatacc
    79981 atcatcggat ggcatcccac caatggctta tatcagcaga aaataatcga tgtaattact
    80041 tttgaccgta acgatgatcc ggtttttggc aagctgatgt tcagaggaaa gggttataca
    80101 gggagtaaaa gaattgtttt tcgatacagt gataaggttg ctatgaagct tcgttttgaa
    80161 gaagcgacat ataatattgt aaaaaagaag gaaaagaaaa aatacaacag ccggatcaac
    80221 aaaaacaatg accgggcttt gcgtaaaccg aaaaagaaaa ccatagttaa aacaaaaatg
    80281 gaagagatga ttgtttttga tatgcttttt cccaccaggc ctgaactgca gggacaatac
    80341 cagtattatt atccgctgtc ggaaacgggt aacggtttct attttaatga tggcagatgg
    80401 gttcaccggt cgcttgagcc agccgaagac actttgctgg taaaaccgat caacaatggc
    80461 ctgatgcctg attgacagag agcagaatca gagaattttt ccaataattt cgcgtgaatt
    80521 gagcccttcg gcttcggctt tgtaattttt tacaatgcgg tgctgcagca cattggatga
    80581 aacggcctgg acgtcttcga tatcgggaga atacttacca tgaagcaatg catgacattt
    80641 ggctccgagt ataagatatt gtgatgatcg cggccctgcg ccccatgcaa tatattgatc
    80701 ggcaatggga gaaacggatg ctgttcctgg tcttgttttg gctgttagat taactgcgta
    80761 ttttaaaaca ctgtcgttga ccggaacccg gcgtaccaga ttttgaaaca tgactaattc
    80821 ttcctgcgta gttaccacat tggcttccgg cattttttca accgtggttc gttttaccac
    80881 ttcaatttcc tcgtccatgg atggataatc gagccaaatg ctgaacataa accggtcgag
    80941 ctgtgcttcg ggcaggggat aggtcccttc ctgctcaatg gggttttgtg tggccagcac
    81001 aaaaaatggg tcgggcaatt gatgggtttg tccggctacc gaaacttcgc gctcctgcat
    81061 ggcttcgagt aatgctgatt gtgttttggg cggtgtgcgg ttgatttcat cagccaatac
    81121 aatatgagca aacacaggcc ctttgatgta tctgaactgg cggtcttcgc caagtatttc
    81181 tgttccgata atatcactgg gcatcaggtc gggggtaaat tgtatccttg aaaacgataa
    81241 tcccaatgtt ttggcaatgg ttgttatcat cagtgttttt gccagtcctg gcactccaat
    81301 aagcagtgca tgacccttgc ataaaatgct gatgagtatt ttgtcaacaa cctcatcctg
    81361 tcctacaata acttttgcaa cttcagattt aagctgattg tataacgttc ttagtccttc
    81421 ggtggcttca acgtcgtttg tataactttt catgtttatt cccatttata tttgaaaaga
    81481 caactttgga agtcatcgat aatttttata taggttgaac tttttttgga agcaatccat
    81541 ttttccaatg cctgtctttt tttggcgtca agcgcaaatt gtgttatcag gttgtaatct
    81601 tcgctcaatg tggctttatg cggaatgcta cggcttttga gaaagagaat cctgtaacct
    81661 tctttgccgt cgtcgctttg aaataagacg ggtttgctta tgctgccggc ttcgagtttg
    81721 tcaacagtaa aaaacacgga tgcatcaact tcggaaggtt tgaattttgt gctgccgcta
    81781 tatggattca aaatcaatcc gccattgctc cggtattctt cgggattgta tttgtccgaa
    81841 gcttcggcaa atgagattgt gccggctaca atctgattat acagggtatc cagtttcagt
    81901 tttcctgcat ataaatcggt gggtgatgct ttaaccatga gcagaatatg acgcacattg
    81961 atataatcac ctttacgatc gatgagttga agaacatgaa aaccggccgg agtttcaatg
    82021 acttctgaca catcacccgg gtttttgagg gaaaaggcag ctccttcgaa ttccggatac
    82081 atttgtccac ggctaaacat gcctaattcg cctccgtttt tggcactgcc gggatcttcg
    82141 ctgtataaac gggcaagtgc ttcaaaactc tcgcctttta aaatgcgggc tcgcaaatca
    82201 gatatttttt gttgtataaa ttgtttgtcg gcagatgatg gatccggaat gacaacaatc
    82261 tgacctatct ccagctctga ctcaacaaag ggaatgctgt cggcactaag gccattgaaa
    82321 aactttttta cttctgatgg tgaaatgctt acattttcag taatctttcc ctgcatttgt
    82381 tgtaccatca actgatcttt aatcagatcc tgaaattcgt ccttgatttc agggattgat
    82441 ttattgtagt atttttcaag cgcttcagga cttccaagtt gctgaatgaa atagcgcaaa
    82501 cgtcggtcaa tttcggcctg aacctgatcg tcggtaacaa ccagtgagtc tatttcggct
    82561 tgagtgagca acattttctg cagcaataat tcttcaagta tagagcaccg aagttcatcg
    82621 gtttgttttt caccctgcat cacgagctgc cagtattgag tttcgacatc ggagagcatg
    82681 ataatttttt cgccgacaat ggcgacaatt ttatcgattg tttcgccgga gggttgagcc
    82741 atggcggctg ttggcagcat taatgcaaaa agcagtggta gaatcagttg tttcatcagt
    82801 tcagtcaatg tgtagagtta cttttttttc tttcaacgca ttttcgtaca atttattacg
    82861 atagtcatcg agcagaatgt ttttcttgcg cgcaatcaga acagattcaa tgcggtcgcg
    82921 taccagtacc agcggagcag gactttcggt aagcatgtat cctttgaagc gcatcagata
    82981 aacaacagaa tcgtttgata attcgtaaaa tgtgttggtt ttcaggaatt cggcctggtt
    83041 ttcggctttg atgggaattt ctttcaggat atcgttgaag tataaccaga cattatcttc
    83101 aagaaaataa ttcagtgctt tttcgcgtgc gatgctcatc agttctgctt tttcgacagc
    83161 gttgtcggac ttgagcaatg tttttatttt cgcaatatcg gttgcttctt tttgcaattt
    83221 aagatattga acttttacaa tgttttcttt cagtaaaaat tcatcctgat tttcgcgata
    83281 gtatttattg atttcttcgt cagaaaccat tattttcaag gagtcagaaa ccagtttttc
    83341 ttcgagtgcc gaaataatca tcagcttttt tgcccgctcc atctgttttt caatttcatc
    83401 catttcctgc tcacagagct ttgtattggc ctcgatgtac atcagttgct caacaatcca
    83461 gctttgtacc atgtttttga gaaagctgac actgtcttcg gccgatatgt tttcaggaaa
    83521 ataaatgtcg gaaatataca attgttcatg atctacctca gccagcaagg tgtctgacga
    83581 ttgtttacga ttgcaggcaa acataaacaa tagcgataaa ccaacaacag ataatatttg
    83641 gcagcggatt atcattattt ttggtaaata ctatcgagaa ctttttcatc aactttccag
    83701 gtgtatttgt tgcggagttc gctcacccat tgctgttcga ggtaattctg atattcggcg
    83761 gttaccagtc ctttgacttc ctgtagcggt ttgggttggg gttcaagcag attgagaatg
    83821 gataccagtt ttatttcatt gttttgacga attaccgaat tttgattagt cacccaaggt
    83881 gtctgatcaa ccagtggatt tacgcctttt tcaactttta caatctgata agttatggca
    83941 ggaatactat ccttattgag tatagagctt attttttcag gcgtatatcc ttttttgggt
    84001 gcttttttca gcagtttctg caatgctttc agctcagttt cgcctttgca ggtgtaaata
    84061 gctgcttctg ctctgggacc atacaggtaa ttctggttga ttgtttcata gaattttttg
    84121 agaccagaag tgtctttcac agcttttgac caaacatttt tgtccatcag gtcaaagagc
    84181 agaatgccat cgcggtattc tttcatcagc gctttgaatt cggggtattt ttcttcgagt
    84241 tttgtgtttt catgctcgat gatcatttct tcggaatact gattgaatat agttttgaca
    84301 aaggtatata tatcgtcacc ttctttgatt tgggattgtt tgtgcatgag ataatcagcg
    84361 aaatcgccca gtaagtaagg atttcctgca atagtgcaca cgggctttga cagggatgga
    84421 gctgccggta catgccagct tgccatgtaa atgctgtcgt tgatcagtgg gaccaattct
    84481 ttcagcacat tttcgtcggc tttgaatttg tattcggttt ttagtttatc aacgagttta
    84541 tcgcgggcaa taaaagctcg tttgtcgcgg ctgatctgtg ttttcagtgt gcttttggat
    84601 tcatcataag tgcctatggg ttttttatcc accagtttga tgatatgcca gccataaatg
    84661 gtttgaacag gttttgatat ttcaccgggt ttcatgtcgt agagtggcag gacaaaaggt
    84721 ggtaccatgc ggaaacttcc aaaccatggc agaataccgc ctttggcaac ggttcctttg
    84781 tcatcggaat aattcttaac aactgtttca aaatccaccc cgtcgagaat ttgtttatat
    84841 gcttcatcaa tgcgggcttt gattgaatct tccttgccgg gaacatcttt gatgaggata
    84901 tgtccggcct gtactttacc caatgctggt ttgcggtcgt ttacaagtat caggtgatag
    84961 ccgacaggag tgcgtacagg attgcttatt tctccggttt tcagattata ggcagcagtt
    85021 tcaaattcat agtaaaggtt taaaacgctg aaatagccca aatcaccgcc attgcctttc
    85081 atcatagatt gaccatcgcg gccgggacgg tctttggcgc tttcatcttc ggagtatttg
    85141 cgtgcaattt catcaaattt acctccggca acaagctttt tccggatatc cattattctg
    85201 ttgtacgctt tgagggtatc ggctggtgaa gcattgggcg acaccttaat caggatatgg
    85261 gatgctctga tatcccattt catacggtcg tatgcttcaa gcagcaactg atcggttact
    85321 tcgcggtttg aaaggtatgg ctgggccagt tgacggcggt atccggccag ttcattgata
    85381 aatgatttcg aagtatcaag ttttaaggat tcagcttcct ttactttgag tttgtagtta
    85441 atgaaaagtt ccagatactc tttaacgttt tctgcgttca gtggttcttc cttgacattg
    85501 tttttgttgt aaacagctaa aaattccgat tttgtcacct tttcattttc aatggtcagg
    85561 agcacagggt cgttttgtgc aacaatgcct gaaaaggcaa gaaaaaaaag aaatgctgag
    85621 aataatactg atttcataag cgtgaaagat tttggttcag aaaatttaac gactatagtt
    85681 gggtgcctcc tggctgatgg taacatcatg gggatggctt tcgatgattc ctgcatttgt
    85741 aattctgaca aactgtgcgt tttgcagcgc ggcaatatca ggagatccgc agtaacccat
    85801 tccggcgcgg agtccaccaa taagctggtg catcacttcg ctcagtgtgc ctttgtatgg
    85861 caaacgccct acaattcctt ctgggacaag ttttttgata tcatcttcgg tgtcctgaaa
    85921 atagcggtct tttgatccat gctgcatggc ttcgatgctg cccatgccgc ggtaggtctt
    85981 gaatttgcgg ccttcgaaga tgatggtttc tcccgggctt tcttcaacgc cggcaaaaag
    86041 cgaaccggcc ataactgagt gtgctcccgc agcaatggct ttggtaatat cgccggtgta
    86101 tcgaatgccg ccgtcggcga tgaccggaat tccggttttt ttcaaagctt tggcaacctc
    86161 gtgcactgcg gtgagttgag gcactccgac gccggcaata atgcgtgtgg tgcagattga
    86221 accgggtccg ataccgactt tgactgcatc gatatcgagt tttgccaatg ccagtgcagc
    86281 ttcggctgtt gctatattgc caacaacaat gtcgattccg gtgaattttt ttcggatggt
    86341 ctttaccata ttcagcactc cgcgcgaatg tccgtgtgca gtatcaataa caatggcatc
    86401 gaccccggct gccacaaggg cgtcgatccg ctcgggagtg ttggcggcaa tgccggctgc
    86461 tgcagctacg cgcaatcgtc ccagtttgtc tttgcatgca ttgggacggg ctttgatttt
    86521 tatgatatct ttatatgtga taagacctat taaccggtaa tttttgtcaa caaccggaag
    86581 tttttcaatt ttatgttttt gtagaatttc ttcagctttt tcaaaattgg tgaattcgcg
    86641 gacagtaacc aggttttcgt aggtcattac cgatttgatc agctttgaca gttttttttc
    86701 gaaacgcagg tcacggttgg taacaattcc tacaagtttt tgatcggatc cgataaccgg
    86761 aataccgccg attttatgtt ctttcatcaa cgccagtgca tcggcaactg ttttgttttc
    86821 ggtgatggtg atgggattga tgatcattcc gttttctgcc cgcttcactt tggttacttc
    86881 aatggcctgt tgttcaatgg tcatgttttt atgaatgact ccgattccgc cttcctgagc
    86941 catggcaatg gccatagccg attcggtaac ggtatccata gccgctgaaa caaaggggac
    87001 atttatttga atgttgcgcg taaacctgga tacaagcgat gtttcgcgtg gcaaaacttc
    87061 actatatgca ggaaccagaa gaacatcgtc gtatgtaagg ccttccggaa ggaatttttg
    87121 gtcttttgga ctcataggta tttctttgtg caaaattaaa aatttatcgc tttccaaagc
    87181 gtgcgttttc catagttttt ggaaatgtag caatatctat gtctgcagac cggactccgg
    87241 ttatttgcca cgcccctgga aaacaatcag tactgtgtat atcagtattt tgaaatccat
    87301 ggccagtgaa atattttcaa tgtaaagaat atcatatttc agacgttcta tcatttggtc
    87361 aacattttcg gcataaccgt atttcacttg tccccaactg gtgatacctg gttttatttt
    87421 atgcaacagc ttgtaatagg gggcctgctg tacaattttg tcaataaaga actttcgttc
    87481 agggcgtggt cctacaagcg acatattgcc aatcagaacg ttgtagaact gtggaagctc
    87541 gtcgagccgg tattttcgca taaaccgccc gaagggtgta atccggctgt cagctttgtt
    87601 tgacagggcc ggtccatctt cttcggcatt cagatacatt gaacggaatt tatgcatgac
    87661 aaagggtttc ccatgaagtc cgatgcgctc ctgactgtag atgaccggtc ctttggatcc
    87721 gaatttcact atcgcagcag taatgaggta caagggcgat aaaacagtca tgaccagcac
    87781 cgatacaaca atgtcaaatc cacgtttgat aacccgctgc cagtgcggca taagctcgcg
    87841 tgaaacgtag attagcggaa catggaaaat ggaattcatt tttacgttgc ccgcaagaat
    87901 gtctttgtaa tcgggaatga ttttaatatc gacagaagta ctgtcaagta tgctcagtat
    87961 gtctgtgatt actttatgtt ccgatggttc aacggcaaca atgacttcct cgatttgctc
    88021 ctcttcgatg atttgttcaa gttcgcttac agagccaaga cgaggcagat attttgaaag
    88081 tttgtcgttg gagttttcga gaacaggaat atagccggtg aaaatatttc cggcactttg
    88141 tgcctgattg gttatgtctt cgtaaatgcg aacggcattg ccgttattgc caacaatgag
    88201 tgtgcggaaa ccccagattc cattgtggat tttgcgtgca gtggcacttg ttattataaa
    88261 ccggccaata taagtcgcta taaaatgtgt ccccaacaaa aacagatagc tttcgtaata
    88321 gttcacaaac gaattcacgc ggtcgtcgag aagaaaaaca aaaaaaatca cagtaacacc
    88381 cataagggtt tgcatggccg tttgacccag ttcacgcaga cgtgaacgac ggaaaatatt
    88441 gcggtaaaaa ccagtgataa aataaaaaag cagccacgaa agcggaataa tgaaaatccc
    88501 gagaaagaaa ttcggatcag ccgaaactac atcccataat tcttcaaatt gcattcctcc
    88561 ttgatatttc ctgaacgaaa acagcgcagc ccaggccgct gcagctccca aaaggtcgca
    88621 gacaacataa cgggcggttt gtttggtccg gttcagtttc attcggcgtg gatcaacttg
    88681 atattgtcga taaaggcctt ggcctcattc atcccctctg gcaattccat ccggaagaag
    88741 aaaaagaagc tgtcggcatc atattccctc gaaacaacag gtgaaagatt gatgtacact
    88801 tttttccatg tattggtttc gttcagaata atcagtggat gataaaccga gtagccgcct
    88861 ttgttggcta ttactcccac aaccaggggg gcctgtgttt tgaagtttaa ttcaagaaat
    88921 atgtagtcgc ctgtttgagg tagtacgtat gatgcatttg atcgaacatc gattgtatcg
    88981 cgctgtgtgt cgatggcgag cattccgctg aaagatcctt caaagacttc gcttggttcc
    89041 gatgtccgga gcatgatggt atcactcagt gttgtgctgt ttatggtcat acctgcatcc
    89101 tcaaaacctt caactactct gaattcaaga ttgtcgtaat acgtcgatga tggttggatg
    89161 atgcttacgg agtccttaac cagattaatg gtggcttccc atggtttaac aaaaggatag
    89221 atggctctgg tattggaaat gccgtttaaa ataatacccg ggtaaatttg aattgtaaca
    89281 ggaccttctt taaggactgg aaaggtaagc ggcatttcaa aagcgcctgt tttttcgcca
    89341 ttgatataca cccaggcatc ggtgaagttc tggttttcgg ttccttctgt actttctgta
    89401 ctgaaagaaa tggactcaac cttgataaat gaaggagttt cttcggaagg gttaatccag
    89461 ttgcacgaag tcattatgac tgctgtcaga aaaaaaatgg caaatagtct catgtggtaa
    89521 agtaaatgca gaaacggcca aaaatgcaga ttagtatgta ctgttaaaat tttttatgaa
    89581 aaaaagtaca gcgtttcatt aatttcttcg ataattttat aggcaacctc caatgcggtg
    89641 tatccatcaa ggattcctac tggtgttggt tcgttgttca ctatcgaatc atggaatttg
    89701 gttaattcca ttttaatggc gttgatggtt tctattggtg gattctcaat atgaatctgt
    89761 ttttttatag caccgttgcc cggatctacc accatggcaa atggcggcaa tttgctttcg
    89821 tcaatcacat cgctcatgcg gatgatttcg gtttttttct ccagaaaatc cacactgatg
    89881 taagcatctt tttgaaagaa acggctcttg cgcatgtttt tcattgatat gcggctggct
    89941 gtcaggttgg ctactgttcc gttgtcaaat tcaatgcggg cattggcaat gtcgggcgta
    90001 ttgctgataa ccggaactcc gctggcactc acttttctga catttgattt tactacactc
    90061 aaaacaatgt caatgtcatg gatcatcaaa tcaagcacta ccggcacatc ggtgccgcgt
    90121 gggttgaatt gtgccagacg gtgcgtttcg ataaaaacag gtttgttgat gtatggctgt
    90181 gctgccagaa atgcggggtt gaagcgttcc acatgcccaa cctgaatttt tacattggct
    90241 tcacggctca gatttaccag tgatgaggcc tcattcagtg ttgttacaat cggcttctct
    90301 atgaaaacat gtttgaattt tttcaatgcc aatgctgcaa tttcataatg cgaaatggtc
    90361 ggagtcacta tatcaacggc atcaaccgca tctatcagtt cctcgcggtt ttcgaagtgc
    90421 cggatgccaa attctttttc aacttccttt gccctttctg catcaatatc atgaaatcca
    90481 agcagttcat aattttcaag ttctttcaga catttgatgt ggattttgcc aagatgtccg
    90541 gctcccagta cccctatacg cagcattgtt cgtttttttg tttaacaatg cgcaaaagta
    90601 gcgataattg ccggatgatt ttattaactt cgcgtgattt ttaaccatta acctgttaat
    90661 tacgtcccgt gatcacatat aaaggaagag tgctggaaga taattaccgt caccagggcc
    90721 tgcggaaacg gctggtggcc gaacttgaac aaaaaggcat caacgacccc cgcgtgctgg
    90781 ctgccattgg aaaaatccca agacattttt tcatggacag cagttttgtt gagtttgcct
    90841 atgtcgataa ggcatttccg ataggtgaag atcaaactat cagtcagcca tatacagtgg
    90901 cctttcagag tcagttgctg cagattgccc caagaatgaa agtaatggaa atcggcacgg
    90961 gatcgggtta tcaggccatt gtgctgatgg aattgggggc taaggttttt tcgatagaac
    91021 gtgtaaaagc gctctacgac cgcacccgcg aatttctgcc ggctatcggt tatttaccaa
    91081 aacttttcta cggcgacggt tacaaaggat tgccgaccta tgcgccattc gaccgcatca
    91141 ttgttactgc agctgcgcct gttattcccg acgcgctttt acaacagctc aaacccggag
    91201 gcattctggt tattccggta ggtgattcgg ccggtgtgca aaccatgaaa accattacgc
    91261 gtgttgatga aaagaccttt accgaaaaag accatggtct gttccgcttt gtgccgatgg
    91321 tggaaaataa acggaaatag ttttcagttt tcagtggtca gtggtcagtg gtgagtggcc
    91381 agtggccagt tatgtcaagg ttgaggttaa ggttttttta tcatagctga cagcttcagc
    91441 tgtcagacca aatag
//
LOCUS       DAOPKE010000002        89841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_220_length_89841_cov_13.720881, whole genome shotgun sequence.
ACCESSION   DAOPKE010000002 DAOPKE010000000
VERSION     DAOPKE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 89841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 89841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220_length_89841_cov_13.720881"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..1475)
                     /locus_tag="PKY63_00320"
     CDS             complement(357..1475)
                     /locus_tag="PKY63_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99083.1"
                     /translation="MKKFPAVCITGPRQCGKTTLAKQLCSATAKDFIYLDLEKYEDLM
                     RIKDAGLFFRDNRDKLIVLDEIQRKPELFAEIRAEIDSFKRSGRFLILGSASPDIIKN
                     TSETLAGRVRYIHLHPFILSELNNPALWKDLMFWGGFPDVVLDNDERFRKEWLDNFIY
                     TYLERDLPMLGLKADPVQIRRLWEMLAWQAGSLLNSSNLGRSLGVSHHTVNNYLDYLE
                     GAFMIHRIEPFDANIGKRIVKSKKIFIADTGLLHRLLRLSSYDQLMGNAMAGHSWENF
                     VLNQLLPLLKNDWNIWFYRTQTGVETDLVLGKGSKPMVSIEIKFSSSPAMTRGFATAI
                     ADLGTLKNFIIIPEGEKFRVREDVFVYGLRQFLSELPF"
     gene            1926..2474
                     /locus_tag="PKY63_00325"
     CDS             1926..2474
                     /locus_tag="PKY63_00325"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HPE99084.1"
                     /translation="MKPGSKFNIDDFIKENINSSDVLSQFKGTISEKKSDRILDNMEG
                     LFGEQETRIRKRILLAASEILQNVSMHNEYPLYPAVFLATRTRQGFKIDCGNAVSITR
                     RTVVEKRIREANRKAGGSMKDNVRQAEAEKPTIADISNGSSGLGLLEIRRKAGMPLDF
                     AFTIIDNETFFFHLIVKFNMGE"
     gene            2545..3036
                     /locus_tag="PKY63_00330"
     CDS             2545..3036
                     /locus_tag="PKY63_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99085.1"
                     /translation="MNSEQSVHSDGSRGTRKCEGRFDLLNISGDTIIKFSTAEMKRVF
                     NLEDRLAVYSATVLKLCNLLPKTYAADVIAKQLTRAGVSVALNYGEAQSAESRKDFIH
                     KVKISLKEARESYVCLKIINLSDITDQNDLFSSLLKEANEIISILVKSVETAKKNMEN
                     NKE"
     gene            3182..4465
                     /gene="purD"
                     /locus_tag="PKY63_00335"
     CDS             3182..4465
                     /gene="purD"
                     /locus_tag="PKY63_00335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPE99086.1"
                     /translation="MKILITGSGGREHALAWRLARDIGKENIFIAPGNAGTVSCGTNL
                     PCSYNDFEKLQEYIISLSIDMVLVGPEEPLVNGFTDLLKKDPRFSKLMIIGPSAQGAM
                     LEGSKDFAKQFMMRHGIPTAAYATFTAEKKAEAQSYIRSHSGPYVMKADGLAAGKGVV
                     ITESADEAVKHIASVFEDNIFGEAGKTLVIEQFLEGIEMSVFALTDGKNYVLLPGAKD
                     YKRIGDGNTGPNTGGMGTVSPVPFATEEFMHKVVTRIVEPTISGLQKDEIDYCGFIFF
                     GLMNVKGDPYVIEYNVRMGDPETQVVMPRIGGSFADMLKAAATGTLTDVKTETLPQTT
                     LSIVLASEGYPGSYQKGKTISLPEGNHLIFHAGTTQSNNQITTSGGRVLAVVGTGNTI
                     SEARNSAYTLAEKVHFEGKTNRSDIGLDLMQYENT"
     gene            4452..5432
                     /locus_tag="PKY63_00340"
     CDS             4452..5432
                     /locus_tag="PKY63_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99087.1"
                     /translation="MKTLNAERTINSQLKFIFILFWLATLFLMLQRGEATTIIRLHPW
                     LGGITETTLDLNFSWIIILRVIFIAVSTILFWLISIRNLELPKKDYMVFFILPVIHFT
                     ITDWAMGLMVSVYWTTFLFILFQLLPGNNSRAVLRKTLTAGVFGGLLLLNGAFALLFF
                     LAGIITMIISQNISFRSTIVWITGFLIPGFFLFLWYFVFDKTNIIFGNSDAFMLEEEM
                     LKFAFPLTFYGCPALLIILSLFAHSRMSESKISVRRSYSAILFSLIALAPAMISSLFN
                     TYILFSIYGLATVFYWTRLMYNTRRKASFIILWLMPFAFPVLYYYLLNYK"
     gene            5438..6040
                     /locus_tag="PKY63_00345"
     CDS             5438..6040
                     /locus_tag="PKY63_00345"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPE99088.1"
                     /translation="MAALLHHFSESASEAGCDEAGRGCLAGPVTAAAVILPQNFFHPL
                     LDDSKKLNEKERNLLREIIEEQALDWAVAFVDHVEIDKINILNASIMAMHLALSKLKT
                     KPAHIIVDGNRFKPFKKIPHTTIVKGDGKYMSIAAASILAKTHRDEFMEQLHKEFPQY
                     SWNENKGYPSPGHKKAIALHGITPYHRKSFRLNEQLKMKF"
     gene            6102..6518
                     /locus_tag="PKY63_00350"
     CDS             6102..6518
                     /locus_tag="PKY63_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPE99089.1"
                     /translation="MRFLIFILITMSCTAFASAQGRVNILGDKGVETLVAKHISFNEN
                     VKGFPGYRIQIYFESGNYSKNKAFGEKGKFMARFPEIKAYVVFQEPYYKVRVGNFRNK
                     LDAEAFKQRIKEQWPEAYIIKDDIELPEIFAPKETD"
     gene            6546..7775
                     /locus_tag="PKY63_00355"
     CDS             6546..7775
                     /locus_tag="PKY63_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPE99090.1"
                     /translation="MRHTRQSMNFPELDIRDYNYHLPEENIAQFPPEKRGGSKILLAH
                     QQPFSIVGFNELKHHIPKNALLIFNETKVIPARLIFNKSTGARIEIFLLQPEKNTDYQ
                     LALSSKQSCSWNVLIGNAAKWKSGDLSIGFQLTGNTTGILTASRTSDNTVLFSWNADI
                     EFSEVLSLLARVPLPPYIRRDTVIEDRDRYQTVFARNEGSVAAPTAGLHFTTEQLNEL
                     NQNGIMQIALTLHVGLGTFRPVKGNIADHEMHTEPFFVSSEQLNQLTENADRPWIVVG
                     TTTLRALESLYIFAEKIKSGESTASAEFNIAQWDKWNSGNTLSRNEAFSILKQLNAGK
                     NTGLFGNTSLFITKGKPVKTADYLITNFHQPQSTLLMLVDAFASEQWQKAYEFAIQNQ
                     MRFLSYGDACLFKNRFL"
     gene            7851..9308
                     /locus_tag="PKY63_00360"
     CDS             7851..9308
                     /locus_tag="PKY63_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPE99091.1"
                     /translation="MDTSTLKLENLKSLMPAEFTPGQIAEAQTIFLKKLSELSHRHYG
                     GKIQTVPKAPVPGFNWFNVWYTPGVSSVSTGIRDNNDLSFELSNRGNLVAVISDSTRV
                     LGDGDVTPSGGLGVMEGKAYLMKYLGGVDGVALCVDSKGKDGKNDPDKIIEFVKMVQH
                     SFGAVNLEDISQPNCFKVLDVLREECDIPVWHDDAQGTASVTLAGVINALKLAGKKMS
                     DARFVLFGAGASNTTIARLLITDGANPANIIIFDSKGSLNKTRKDIESDTRFYRKWEL
                     CCSTNPQCTSTLEEAMNGADVLISLSTPGPETIKQDWIRRMAPKSIVFVCANPVPEIY
                     PYAAKEAGAFIVATGRGDFPNQVNNSIGFPGILKGALMVRARKITDNMAIAAAHSLAG
                     YAEKRGIDPENIVPTMDEADVFPFEAADVAMQAIKDGVARKPLSHEEAYQWAKKDIEY
                     SRNLVKSMSDGGFLPDPPAAVIEQALEFAINKVKK"
     gene            9310..9891
                     /gene="ruvA"
                     /locus_tag="PKY63_00365"
     CDS             9310..9891
                     /gene="ruvA"
                     /locus_tag="PKY63_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPE99092.1"
                     /translation="MIHFLEGKVAEINPAYVVINCNGIGYFVSISLNTYEKIQGQQNV
                     KLLTHHIFREDGQFLFGFHSEEERVVFRLLLGVSGVGANTARVILSAMNPSGVVEAVS
                     SNNVALLQKVKGIGQKTAERIIVDLRGKIDKGAYISENISPQHNTIRQEALSALAQLG
                     FARQAAEKAVDQALKSGDLTSVEDVIRLALKYL"
     gene            9911..>11343
                     /gene="sprA"
                     /locus_tag="PKY63_00370"
     CDS             9911..>11343
                     /gene="sprA"
                     /locus_tag="PKY63_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPE99093.1"
                     /translation="MKGLVYFPVVTTIALVAVQMFTPAPQKRKISFEPRQVITMPADS
                     DSLAYPFQDDPFYSTQDNNHPLFLNDPKNIKDTVEFDPEKNEYIFKRQIGSIDYRPSF
                     RMDQDEYFKYDFDKAIRDYWHDRAMASGKRNGAGGISINIPGMGGESIFGSNTIDIRP
                     SGTVELIFGVNANRRDDPALNVKQRRTANFDFQEKIQMNVQAKIGDKVDFGVKYNTES
                     SFEFENKMKLQYEGKEDEIIKSIEAGDVTLPLNTTLINGSQSLFGIKSKLQFGKVTVT
                     SVISQQKSQTSNITVSGGAQTSEFELYCDEYEEKKHFLIGHYFRNNYNQWLENLPIVG
                     SPINITKIEVWVTNIGAATQENRNIVALMDLGEQTPYNPVVPLSMNPDAEFPDNESNG
                     MFDIIDVNQVRDINSVNSYMNSTGLVAGTDYEKIENARLLSPTEYTYNSKLGFISLNS
                     TLNDDQVLAVAFQYTILGYDSVFQVGE"
     assembly_gap    11344..11443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11444..16936
                     /gene="sprA"
                     /locus_tag="PKY63_00375"
     CDS             <11444..16936
                     /gene="sprA"
                     /locus_tag="PKY63_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPE99094.1"
                     /translation="FFDFIDNAATNGGTINSKNGRIFFPLLEPFGEDLRELLGDAALA
                     DKYCYDSLYTMTKTGARQFPNKNRFTIAGAFKSSVGNEISLNAMNIPQGSVKVTAGGI
                     LLAENVDYTVDYTLGRLKIINEGILNSGAPINISLESNSLFSIQTKTFLGTHIDYDVN
                     KNFLVGATIMNLTERPYTYKVNIGEEPISNTMWGLNLSYQTESRLITKLVDFLPMIET
                     KAPSRVTIDAEVANLIPGHARAIGKTGTAYIDDFEGSKSSIDIKNIGTWFHASIPQGQ
                     PSFFPEASNDSTLTSGFNRARLAWYVIDPLFFRDNNLTPDHIANDPAMLSNHYMREVL
                     ETEVFPKKENVNNIVTNIAVLDMAYYPTEKGPYNFDVNPTAVSAGIDADGYLLDPSTR
                     WAGMMRKIETPDFEETNVEYIEFWMMDPFIYNPAHTGGKLIFNLGDISEDVLRDHRKS
                     YENGLPTSSVIENVDTTQWGRVPILQALVNSFDNDPTSRQYQDIGLDGLSDADEASFY
                     ATAYLDAIASVFGTTSQAYSKAADDPSNDNFHYFRGSDYDADELNILERYKMYNGVDG
                     NSPTSDQSPEDYPTSSSTLPNVEDINRDNTLNESERYFQYVIDMQPSKMVVGQNYITD
                     VFNASANLADGTTANVKWYQFKIPVKDPDQVIGDIYDFKSIRFIRILMREFSEPIVLR
                     FATLELARSQWRKYENALLYPGEYIPGDEQNGTSFDISAVNIEENGSREPIPYVLPPS
                     IEREVGWGSNTMYEMNEQALVLKTCNLIDGDARAVYKTCEFDIRQYKTLKMFIHSELS
                     QPGAYTLNDGDLSVFIRLGTDFTDNFYEYEIPLHPTDLNVSSQNADINHLVWADSLDL
                     NLDSLIELKKARNIAMRQNNSGVTLLTPYYKYDGLNKMTIVGIPNLSDVRTIMIGIRN
                     PKRNPVNLSDDGQAKCAEVWVNELRLTDFDEDGGWAATTRITADLADIGNIAVVGNMS
                     TAGFGSIEKKVSERQKENIMSYDLATNIELGKFLPEKTGLKIPMHFDVSETFSNPQYN
                     PLNPDVLLTEDLNTYSDPVERDSIMQLVQSYTRRKSLNFVNVRKEKTGTSTKNRIYDL
                     SNFDLTYAYNEVFIRNIDVEYNLTKTYRGALGYNYTSTPKNYIPFKKVKFLNKKALRF
                     FGDFNFYLLPKSLSFRTDLDRGYEENLLRNKSEALILIEPTYLKTFSWNRTYSLKYDL
                     TKSIKIDYIANANARIDEPPGSIDKADSDYEAKMDSIWQNIKSLGRVTNYNQSLKINY
                     ALPINKLPMLDWISSSVQYGGDFKWTAAPLYKDTLGKYAESPYGNTIENNRSISANIN
                     GNLVNLYNKIPYLKKLNQASTKPKQNVNSRAEAMLNDTTGNAKKDTIQLFKKIADNVL
                     KLMMMTKNASLSYTLAEGTYLPGYRGGMDILGMDLDNQAPGWGFVFGDQRDIRPSLVQ
                     HNLLSKDSLFNNAYAKRKNENLNARVSIEPVKNMKIEITAMRNFTENHSEYFKFDEAS
                     NAFRSYSGIDNGSFSLSIVTWSTAFIQDYDDYSNKTFEYFKTIRPEIAAQVAQNSPIW
                     DGTYVTDSTTGQIYPNGYGPTSQDVLIPAFLAAYTGNNPEKYISSKFPRIPLPNWRLT
                     YNGLTQIPWVKKRFKSATISHAYRCTYNIGGYSSNVLFSDSDADGYTTVKDALGNYLP
                     KFEIAQVSITEQFSPLMNIDLTWKNSLISKIEYKKSRNLAVSFANNQMTEIISGEITL
                     GFGYRFKDVEFEISGKDFKSDLSLKADFSIRNNKTVLRKIVEDVDQISAGQKVISINL
                     SAEYMLSQKFTIRAFFDKVINNPYISSQFLNSNTNAGISLRFSLAQ"
     gene            17004..17384
                     /gene="gcvH"
                     /locus_tag="PKY63_00380"
     CDS             17004..17384
                     /gene="gcvH"
                     /locus_tag="PKY63_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPE99095.1"
                     /translation="MNIPENLLYTKSHEWIRVESETGFIGVSDFAQSELGDVVFVEVE
                     TVGETLDREEALGTIEAVKTVTDVYMPVGGEVLEFNEKVKETPDLINKDPYGEGWIVK
                     IKISNPTELNDLMNAESYKGHTGH"
     gene            17516..18199
                     /locus_tag="PKY63_00385"
     CDS             17516..18199
                     /locus_tag="PKY63_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPE99096.1"
                     /translation="MDIKKNPKYDLERYKGLFLEAGLVIALGLMLMAFEWRKYDNSND
                     GFEQMQVVEIPEEMVEITRQEEPEPPKPEPQTTLLEIVDDDIEVQDELDINAEDDQDT
                     EVGDFVAPVDDGEEVEIVEAEIFTIVEEQPGFPGGESALMDYLRNNIKYPQLARESNI
                     QGTVFVTFVVEPNGAITNVKVLRGIGGGCDDEATRVVKNMPAWTPGKQRGKPVRVQFN
                     LPIRFILAG"
     gene            18344..19021
                     /locus_tag="PKY63_00390"
     CDS             18344..19021
                     /locus_tag="PKY63_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPE99097.1"
                     /translation="MDTKKNKSKNLENFKSMFFETGLVVSLFLVLAAFEWKTSPSVNS
                     TLTETPYVYIDTDTVIKFKTIEKTPPPKVITEIKVTKKEVETTPEIFNTEWDDKASEK
                     VFTEIKKDETEPQVEGDEIITVPPLMPQFPGGDEALMDYMMKNTKYPMQDREIGLQGK
                     VYVSFVVEKSGEVSQVKLERGIGGQCDGEAMRVVKNMPSWVPGKNSLGRPVRVKLTLP
                     VSFILIN"
     gene            complement(19018..20253)
                     /locus_tag="PKY63_00395"
     CDS             complement(19018..20253)
                     /locus_tag="PKY63_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPE99098.1"
                     /translation="MKIEITAPVGSYESLRAALEAGADSVYFGIGNLNMRSSSAVNFG
                     QEDLANIVRLCRESGVRAYLALNTVVFDNEADEVNSILQSAANAGIDAVIATDVSVLE
                     RSRELGLTVHMSTQSNITNAGAVRFFSRWADVMVLSRELSLEKVKQLHAKIIQQNICG
                     PSGNLVRLEAFAHGALCMAISGKCYLSLDNLNRSANRGQCAQICRRPYLVRDLEGGTE
                     LLIDNEYIMSPRDLCTLGFLDKLIAAGISVLKIEGRGRPPEYVSAVVRVYSEALKAIE
                     NNTYTDAAIASWMERLSKVYNRGFWEGYYMGKTIGEWTDRHGSSATERREYIGKVTNY
                     FSKLGVAEVRAETGPVFPGTTVTITGPTTGLYEGEIDKIHLDRGEAPMAPQGALFAFS
                     VSRMVRRGDKVFRIVQVES"
     gene            20365..20865
                     /locus_tag="PKY63_00400"
     CDS             20365..20865
                     /locus_tag="PKY63_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99099.1"
                     /translation="MKKIFLLLGIAFAATMISCGPTTEDAIKHNDNIVADQKEMLDLE
                     TDLLNDIVDGQEAADIKKTFKKYLGFLEEMEKKYNEMDEFDEKDTFRKAMINLIKEFK
                     SVAENEYDEMIEIYSKEPDALTDADFEKWEDLTEIIDEKEGKANDDFLDAQKEFSKEY
                     NFDLKK"
     gene            20998..21567
                     /locus_tag="PKY63_00405"
     CDS             20998..21567
                     /locus_tag="PKY63_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99100.1"
                     /translation="MKKLIVITAFIIAAFSSCGPSEQKRQDANDVYSETLGIINEVNT
                     HDSEYVYCMQYLLREIQSPDLKKNKKKLKSITDSISLLDAACDSLAIVIAEAKTKTEK
                     LNVEKPDMELTEAADSLLSKYNTITQNIYQDINQKMKKISLPVKDSEYTALLRLSYRA
                     DSVLNAAVTNFNTESAAFYEKYGLKDLKK"
     gene            complement(21595..23346)
                     /gene="aspS"
                     /locus_tag="PKY63_00410"
     CDS             complement(21595..23346)
                     /gene="aspS"
                     /locus_tag="PKY63_00410"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPE99101.1"
                     /translation="MYRTHTCGELRQENIEQTVVLSGWVQRIRDLGGMTFIDLRDRYG
                     ITQLIFDLDANKDLCETARSLGREYVIRVTGIVRERSNKNKNINTGDIEIEVQKLEVL
                     NKSQVPPFTIETDTDGGEELRMKYRYLDLRRAPLQFNIQLRHRMALEVRNYLSENNFM
                     EIETPYLIRSTPEGARDFIVPSRMNTNQFYALPQSPQTFKQLLMVSGFDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFVEREDVLNVFEGLTRQLFRKIKNTELPEFIRMPYSE
                     AIRLYGSDKPDLRFGMQIQDISEIAKGKGFQVFDSADHVLALVVPGAAEYTRKQLDEL
                     TEWVKRPQIGATGLIWAKFSSDGTAKSSVDKFFTADDLNAWKSSCAAGEGDLMLIVAG
                     NKKKIFGIAGDLRLELGNRLGFRDSSTYAALWVVDFPLLEWDDDAQRFFAMHHPFTSP
                     VAEDASMLESDPGSVRANAYDLVINGVEIGGGSIRIHDRGLQMRMFRALGFTEEEAAD
                     QFGFLLGAFEFGAPPHGGIAFGFDRFAAMMAGQNNIRDFIAFPKNNSGRDLMADSPSF
                     VDQKQLDELSIRLVKPE"
     gene            23419..24594
                     /locus_tag="PKY63_00415"
     CDS             23419..24594
                     /locus_tag="PKY63_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99102.1"
                     /translation="MADRILLFFLFLLSIVVANSQENLIYNGDFETYRNCPEDYTHYL
                     EGITELAPGWFISNTSTPDYFNRCSIKSKVGIPANFAGNMEPHSGNAYVGMILRADSS
                     RYPYSRGYNEHLQTVITMPLERNKLYCLEFYYVLSNNSGIASNGLSVYLSNAKPVFDE
                     AAEKFPFVPQLEIRRDSVLNERNQWNLFSGVFRASGEEKYLVIGNFTDYTETKYVPIV
                     EQATTQIHSFAYYLFDDFRLYRIDNEDECLCNVIQNQTDSFPPIQFAKEDSVFMEDIE
                     IGETIILRNVYFEFDKTELLPYSFAELNKIYNILIQYPAMTIEISGHTDNIGTERYNQ
                     ALSEERAKAVLEYLYNKGIELKRMRYAGNGSRLPIADNNLPEGRQMNRRVEIKILSK"
     gene            24594..25034
                     /locus_tag="PKY63_00420"
     CDS             24594..25034
                     /locus_tag="PKY63_00420"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPE99103.1"
                     /translation="MENQDEKFMQEALKEAFKAFANNEVPIGAVLVHNNRIVARAYNQ
                     TELLKDVTAHAEILLITAYSADSGMKYLEDFTLYVTLEPCAMCAGAMKWARIGRLVYA
                     ADDLKFGYTTLSENILHPKTNITKGVFQKESAQLMKDFFAKKRL"
     gene            25135..>26528
                     /locus_tag="PKY63_00425"
     CDS             25135..>26528
                     /locus_tag="PKY63_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99104.1"
                     /translation="MSRNIFLTFLLVFTAVSLMSQPIDDIAKMQNDKKYRDAYDKAEA
                     ALLDFNYEEALEIYLKWDSVYPNNANLNFKIGYCYANMSSDKPRAIPYLEKAASSVVR
                     EYVGDYIESNAPVDLFMLLGIVYHIDYKFNKAVENFDKFIKYAEFDDPELVDSVKVLK
                     EKSYTAIRVINNPLSIKIENLGELVNTNYPEYSPVITSDMKTLYFTSRREGSTGDKKD
                     AKGLFFEDVYYSSLGSDNRWSTAKHIGGKINTVGHEATISISPDGKTILLYRDDKGDG
                     NIYFSELLENNEWSKPEKLPEPINSKSWETHACFSPDMNFIYFVSNRPGGYGGRDIWV
                     SEKIGGHWTEAENLGPTINTPYDEDSPYMLPDGMTLYFSSQGHENMGGFDIFISTISE
                     DGFWSTPENMGYPVNTTEDDVFYIPTPDEKHAYYSSSKNYGIGGQDLYYMTIVKAKKK
                     TIALRGRVADANSY"
     assembly_gap    26529..26628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26629..26898
                     /locus_tag="PKY63_00430"
     CDS             <26629..26898
                     /locus_tag="PKY63_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99105.1"
                     /translation="LNRLVKLMTDLPTLKIEIMSHTDNIGSDAYNLDLSNRRAESVVS
                     FLIKSGISKDRLVAKGYGESQPVATNTTDEGRQANRRTEFRILSK"
     gene            26951..29131
                     /locus_tag="PKY63_00435"
     CDS             26951..29131
                     /locus_tag="PKY63_00435"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPE99106.1"
                     /translation="MKKLFFVFLAAILSISKVSADEGMWLPLLVDRLNYTDMQKMGLQ
                     LTAEEIYSINHASLKDAIVSMNDGMCTAFMVSKEGLMMTNHHCGLEYITENSIKYNSD
                     YVQNGFWALDKKQELYNPNLKATFLVRVEDVSQKVLSQLKETMTPAEREAKVNEISAK
                     LVSDAVNGSHYEGEVKSFYKGNEFYLFIYEVFRDVRLVGAPPMALGNFGADADNWLWP
                     RHTADFAFFRIYMGPDGKPSPYDKRKNIPYVPKYYIPVSTQGVNEGDFTMVLGYPGTT
                     DRTMTSSGLNLTLETINPSIIKIRDSKLRLMKEDMDRDPSVQLMYRSKYNRSSNYWKY
                     YIGQIEVLKRLKAIDQKKQMEADFTKWVNADASRKSIYGNALAAIDLATTEIGKYRKA
                     VIYYRECVFRGPEIISFANKFDSLYYYLNKTDIPKEQKAAKVADLSTKLKYYASNYYF
                     NGYNIDVDKKIFIALLRFMKDDVGAEFYPTVFEEVDKKFKDNFSMYANFVFEKSIFSS
                     KEMVYEFLREPNAKVLEKDPGFIAMLSFNEKYDQIKEQIAVIEQKLENSERLYMHGLM
                     KMLEGKKKFYPDANSTMRITYGKVTNYTPTDGVEKPFYTTLSELIAREDAKNPDFIVP
                     EKLKDLYNRKEYTNYANAAGDVPVCFLTDCDVTGGNSGAPILNAKGELVGIVFDINWE
                     ATASSLSYVPDQTRTISTDIRYILFLIEKYAEATNIIQELDIRK"
     gene            complement(29168..29683)
                     /locus_tag="PKY63_00440"
     CDS             complement(29168..29683)
                     /locus_tag="PKY63_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99107.1"
                     /translation="MLFDSCRATADYSANVLLKKPELLQIFETMAFSDEYPFDMRASN
                     VIEKADDLRPGFARQLIPKIIRHLTVFKNDGPRRQFLRMLSRYVDEMDENDTGLLYDV
                     CFAFACDKAQPVAVRHNALLVIESICRRYPDLKNEICSALRLRINEESGAFGNWVKAF
                     TNADFNAIVKK"
     gene            complement(29715..32162)
                     /gene="priA"
                     /locus_tag="PKY63_00445"
     CDS             complement(29715..32162)
                     /gene="priA"
                     /locus_tag="PKY63_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPE99108.1"
                     /translation="MFAEIILPLPLKGYFTYQIPDHLKVQVEIGMRVVVNFGAKKFFT
                     GIVARITDQKPGEEMNIKEISDVLDVFPVVNPLQLQFWEWMSSYYMCPQGDVMNAAIP
                     AGLKIESNTIYVIHPDFNDDFSQLSDDEYMIAEAALKAEKLTSVDIQAITGRQAVHHL
                     IKSLIDKKVLHPVEELEERYTPKKQKFVAISAEYENEGVLNALMVKLEKKSENQFRAL
                     LTYFNLARGQNVVSFTELNKVAGAAAVTALIKKEILVQSERIVSRLDEKSDNGPAPVF
                     QLSEAQDNAYSDVKVALDQNKPVLLFGVTGSGKTEIYIRFISDMLAAGKQVLYLLPEI
                     ALTDFMVDRLRAYFGKDLLVYHSRFNAQEKVEIWNEVLEKSEPKLILGARSALFMPFS
                     NLGLIIVDEEHDSSYKQQDPAPRYHARDCAVMLSHMTGAGIILGSATPSLETWVNAAN
                     GVYARVELSERFGVAVYPEVHVIDIRQATFRKKMLGPLSPELYEAVKKAVMHKRQVIL
                     FQNRRGYSTWIECKECGWTPVCRNCDVSMTYHKNVELLKCHYCGYTDRVPSKCPACKS
                     SQIVMKGMGTQRIEDELQILFPDYKIARLDMDTTRGKKAMSEMFKGFDEGRFNIMVGT
                     QMITKGLDFSRVDVVGIMNADNLHAFPDFRSHEHAFQTMMQVAGRAGRRDAGGKVFIQ
                     VYDKDNPIVNFVAEGNYVGFMERETQSRMNYLYPPFVRLIRLSVMHKDKPVADKASAM
                     MKALLKKQFNEKQLLGPEFPPVARIKNFYIKNILIKLPRNKELGSYKKWISAQIAELL
                     AHPDNKGVRVITDVDPL"
     gene            complement(32172..33155)
                     /gene="pfkA"
                     /locus_tag="PKY63_00450"
     CDS             complement(32172..33155)
                     /gene="pfkA"
                     /locus_tag="PKY63_00450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPE99109.1"
                     /translation="MNEIKRIGVLTSGGDAPGMNAAIRAVTRTAIFHGLHVTGIRRGY
                     QGMVEGDFIELETKSVANIIQRGGTMLKTARSKDFMTKEGMDRAYNNLRHAGIDALVV
                     IGGDGTFRGAYEFTAQYKDIKVIGIAGTIDNDLAGTDNCIGYDTALNTVVEAVDKIRD
                     TADSHERLFFIEVMGRDAGFIALRSGIAVGAEDILIPETSSSIDELIDKLENGRRRNK
                     TSGIIMIAEGDESGGAFVVAEQVKKRLPKYDVRVTVLGHIQRGGSPSCMDRVYASQYG
                     YHAVKALLAGKSGVMVGIIKRDVVETPFGEAVKHTNKVRTDLKEMIHILSV"
     gene            complement(33237..34238)
                     /locus_tag="PKY63_00455"
     CDS             complement(33237..34238)
                     /locus_tag="PKY63_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPE99110.1"
                     /translation="MNLKRIIISRTDAIGDVILTLPLAVFLKQRFQDCTVIFLGRNYT
                     SAVVQCCSEVDEFVSWDDMSDNPAESLRKLNADAIIHVFPRKEIAKAALKAGIQLRVG
                     TSHRIFHWRNCNRKVSFTRKNSDLHEAQLNFELLKGIGISHVPSLDELNAMQLLLPNS
                     ILRVDLNELIDKSKFNLILHPGSRGSAREWGIDRFAELMKLLPADKFKIFLTGTEKEG
                     ALFRSELAEPFPQVTDLSGKLSLSELIVFVQHCNALLATSTGPLHIAAALGIHAIGLY
                     PPIRPMHPGRWAPIGKNTKVFVMDKECSDCRKTAHCACMREISAAEVAGYIHAVVAG"
     gene            complement(34582..35040)
                     /locus_tag="PKY63_00460"
     CDS             complement(34582..35040)
                     /locus_tag="PKY63_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99111.1"
                     /translation="MKSLKTLRIIHIAILIEALVFFVIVFFVNIDKDLIWMNDENPDL
                     FLIGLILVVTSAMGSFIVPRIMLNSVRSKESREERMKAYLSVAIVRLAFIDAAMTISI
                     VLFFVEKAWIYALMLALLIVVYLGMYPTQNAVERDTELSTGDFSEDEFND"
     gene            complement(35051..36616)
                     /locus_tag="PKY63_00465"
     CDS             complement(35051..36616)
                     /locus_tag="PKY63_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPE99112.1"
                     /translation="MKIRILVLCIFAAIVASAQTTVPTQLNVFKNGTYFIMREGSASI
                     SNYEASVMVPPGPLLSTYWISTTKDVKIEQIRIVNDTLKNKRNFFSYNELLKANVGKK
                     LRVVYMLDEKNIRELSGTLSDFNTSASTLKVKGTDNRTTFISSSFIKEISFDENPIEK
                     AEVDSIVRRGFVTFSKQSGSVPVRVSYMQSGIQWTPCYNIKILNDKEVQLELKATVEN
                     NMEDDVVNTDLVLTVGSPNFYYGTQLDPIATSYLTGYGWGAGGTAYNQFSNARFDNYQ
                     VQTDDMTMSESTAGGVDYNNYYNYNTGGEKSNDLYFYKCGKVTLPKNTKTSVMIFSQN
                     TTYKDIYEVSIGDLVNYQGNRYVMDTEKNRQTVFHSLRLTNATTQPFTTGPVFVLDEN
                     LMPLAQDQLKYTPVKGETSVQLARSSDIVVTYTEEEQSRTDNYTTINRTKYAKVVIKG
                     EIKIENLQEKVINLKVDKTIYAAVTESSDAGVSKKTGSYYGVNPLSEIKWDLSIKGGE
                     KKVLTYTYEVLVP"
     gene            36663..36845
                     /locus_tag="PKY63_00470"
     CDS             36663..36845
                     /locus_tag="PKY63_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99113.1"
                     /translation="MKIIIEPEILQKKELPDHGVFQTRPISVKATIIKILTRIQQYKV
                     MARAKEKSSDTVLRFS"
     gene            complement(36831..37946)
                     /locus_tag="PKY63_00475"
     CDS             complement(36831..37946)
                     /locus_tag="PKY63_00475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPE99114.1"
                     /translation="MAELNINIDRITGNINSISHFLKSRNLMWSLITKVFAGERELLE
                     HILSKERIENIHSVGDSRISSLRNIKEVCSDAKTIYIKPPATAYAEDVVRYADISLNS
                     SLRTIVKLNDFAKKMKKRHQVIIMVELGELREGVLRDNLVDFYNRVFNLDNIEVIGLG
                     TNLGCMYGVEPTYDKLLQLSLYRQVLELRFNKSLPVLSGGSSITLPLVDAGKMPADIN
                     HLRIGEAAFFGTSPLNNELFRDLNGNTFEYYANIIELAEKDRVPDGIISDGNVGHTID
                     FDSSGRSRKTIKAILDFGLIDVDMNSLKPIDKSVKFVGITSDMTVVDVGTNRDKKGSK
                     KYHVGDTIVFTPDYMGVARLLNSRFTGKRIQAESAES"
     gene            complement(37948..38391)
                     /locus_tag="PKY63_00480"
     CDS             complement(37948..38391)
                     /locus_tag="PKY63_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE99115.1"
                     /translation="METRIIKQMSDFTEPFSRKDFSDFLFTYLQPFGDPIEDIEKCID
                     YALLDNDKAGGFAISVIENNKIIGGLVVNRTGMKGYIPENIIVYVAVDSQMRGKGIGK
                     MMIEKAIDLCDGDVKLHVEYDNPAKRLYERIGFKNKYADMRFKKS"
     gene            complement(38798..39163)
                     /locus_tag="PKY63_00485"
     CDS             complement(38798..39163)
                     /locus_tag="PKY63_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPE99116.1"
                     /translation="MRIDKFLWDVRIYKTRTLATEACRNGRVSIKDQEVKPARDVAVG
                     DIIVIKLHAFTRTIQIVDFPKSRVGAKLLPNYILELTPEEEFKKLDMVRERLVLQRPK
                     GTGRPTKKDRRELDDWMEE"
     gene            complement(39457..40761)
                     /gene="der"
                     /locus_tag="PKY63_00490"
     CDS             complement(39457..40761)
                     /gene="der"
                     /locus_tag="PKY63_00490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPE99117.1"
                     /translation="MSNILAIVGRPNVGKSTFFNRLVGERAAIEDPTSGVTRDRHYGK
                     SDWNGVHFSVIDTGGYVNNSDDIFEEEIKKQVHLAISEADVILFLTDVREGFTPMDED
                     VADMLRKCKKPVVLAVNKADNNAQALNIGEFYSSGLSEIFAISAVNGAGTGELLDEVV
                     KHFNPMEAEPDPGVARIAIVGRPNAGKSSILNTLIGEERSIVTPLAGTTRDSIFTEYN
                     KFGFHFMLVDTAGIRKKAKVSDNVEFYSTVRAIRSIENSDICLLVVDATRGLEAQDLH
                     ILNIINENHKGIVIAMNKWDLVPKDHKTMEEYKENLIARLMPQTDVPVIFTSATEKTR
                     VIKMLEALQMVHENRGRRIPTSKLNEVLLPILTETPPPMYKGKRVKIKYITQLQLAYP
                     TFVFFCNLPQYVKEPYRRFVERKLRENFDLKGCQIQLFFREK"
     gene            complement(40763..41635)
                     /gene="era"
                     /locus_tag="PKY63_00495"
     CDS             complement(40763..41635)
                     /gene="era"
                     /locus_tag="PKY63_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPE99118.1"
                     /translation="MSHKSGFVNIIGNPNVGKSTLINALTGEKISIVSPKAQTTRQRV
                     LGFLSTEDYQIVFSDTPGYVENPAYELHRKMLGYINAAFEDADIVILLIEPGTRELNP
                     NLLLRLQNLTVPLIVCINKIDLSQQEKMMELMKQYEVVFPKADVMLISALHNFNVDVL
                     LGRVIELLPEHPAYYPKDVLSDRHLRFFVSEMIREQIFYLFEKEIPYHCEVIIDYYKE
                     DENIPKIGATIFVSRESQKRILIGQGGSAVKKLGTLSRKHIEEFLGQHVFLDLNVKVK
                     DWRDDETTLKQMGF"
     gene            41714..41785
                     /locus_tag="PKY63_00500"
     tRNA            41714..41785
                     /locus_tag="PKY63_00500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41746..41748,aa:Met,seq:cat)
     gene            42630..44213
                     /locus_tag="PKY63_00505"
     CDS             42630..44213
                     /locus_tag="PKY63_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPE99119.1"
                     /translation="MKTFLLLSAMMFVFIASMAQKSESDKDCWYNKDMEKNKVPGVSS
                     DRAYDELLQGKTPKPVIVAIIDGGTDFNHEDLKEVIWTNEDEIPGNGIDDDKNGYIDD
                     IHGWNFLGNPSGENVDNDNLEVTRVYRLLKPKYENADSAKMASDEEFQLYLKCKKEVE
                     TKYAASTKALANVTTLYQNLKVADSVFTAYFGTNKYSANQIKTIKDERMQPVVDFMSY
                     WMKKDVDTTIVKGWIDHYESQALYRYNVNFDSRTIVGDDWTQNNNPYYGNNDVKGPRA
                     GHGTMVAGNVAAVRGNGIGPDGVTSNAQLMILLVVPDGDERDKDVANAILYAVNNGAS
                     IINMSFGKGYSPQKHFVDSALRVANKHDVLIIHAAGNDGENVDVEIHYPSNFDENGNI
                     INDKFIVVGASTNSAGKKLPASFSNYGKKSVDIFSPGQDIYSCAPGNKYELASGTSMA
                     SPVATGVATIIRAYFPELTAKEVKEILLQSAVKYDRKVLLPGGSKKKVSFSELSATGG
                     VINAYNAVKMAQERVNAKH"
     gene            complement(44436..44999)
                     /locus_tag="PKY63_00510"
     CDS             complement(44436..44999)
                     /locus_tag="PKY63_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPE99120.1"
                     /translation="MAKNDDRSSALEYSDESAATRIYCIRGRKVMLDFDLAILYQVST
                     KALKQAVRRNIDIFPDNFMIELSKEEFENLRSQTVTSSWGGTRYLPFAFSEYGILQLA
                     NVLRSDIARKMSIRIIEVFVKMREMINVQQEILKRIEMLDVKANGHDQQLALIFEYLD
                     QLEKAKEIDSDYQNRKRIGFRTSLQDE"
     gene            complement(45178..45690)
                     /gene="tnpA"
                     /locus_tag="PKY63_00515"
     CDS             complement(45178..45690)
                     /gene="tnpA"
                     /locus_tag="PKY63_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPE99121.1"
                     /translation="MSQSFRKLYVHIVFSTKVRISFIDDQIEQQLFAYLGGIAKNMEC
                     PPVKIGGYRNHVHILCILSQNVLLMKLVEKIKSGSSQWIKTQGRKYQHFYWQSGYAAF
                     SVSPGDVQGIKDYIDSQKEHHRRITYQEEFLDFLKLNQMEYDERYICTSVSPLQGSAY
                     FSFSRPFGPG"
     gene            complement(45785..46201)
                     /locus_tag="PKY63_00520"
     CDS             complement(45785..46201)
                     /locus_tag="PKY63_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE99122.1"
                     /translation="MELQFESVNANDEKARMLVLTGEEEPKKKRNPEKNIVYLEGVVT
                     SEPFPKQFANGNKLALFTMSYFNDYRTRTGEQVRITEWFQVVSWNKVADVVMSTVHKG
                     ARVRLKGKLRSSAWKDDAGVSHRRVQIVATEMKKAA"
     gene            complement(46589..47173)
                     /locus_tag="PKY63_00525"
     CDS             complement(46589..47173)
                     /locus_tag="PKY63_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPE99123.1"
                     /translation="MSKIFNPEKHESLSSAERRKLIPPEKILDMMHLQKGDVMLDAGA
                     GKGYFAIPALEYVGDTGNVIAADISQQMLDLLMSDAPGSLNLKTLLCEKDNIQLPEKS
                     VDKLLMAIVLHEVDDAVAYLKMLRQILKADGKLFIVEWAPVESPTGPPLHERLSRETI
                     EKYFSDSGFEMLRFENVNDFQYFCEAKKTIDGQS"
     gene            complement(47184..47654)
                     /locus_tag="PKY63_00530"
     CDS             complement(47184..47654)
                     /locus_tag="PKY63_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPE99124.1"
                     /translation="MLRSIFKFFTSLFVPLALGYIAGQYTSEVVPGWFASLNKPVFNP
                     PGWVFGPVCTLLYILMGLSFFYIWIMPRTKERTVAKIIYLIQLALNFAWSFLFFYYQN
                     IALALIDIIVLDVVVVIMLLNFYRLKPIAAYLNIPYLLWILFATALNAAFYFLN"
     gene            complement(48045..50633)
                     /locus_tag="PKY63_00535"
     CDS             complement(48045..50633)
                     /locus_tag="PKY63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPE99125.1"
                     /translation="MEQPATETKTRKKRKWYGIALRIFLWLIGSVVFLILLAWLLVFI
                     FQNKIEQKVIGQINKELNSEIKVKDVDVTLFHHFPMVSVVFNEVSASDATQQKTGNLF
                     SARWVSLEFNLIDMITGDYTIRQIGMDRGSVWLRVFKDGSDNFHILKPTTDTTTSSES
                     TAFHLERIYLSDVRGGYFDERFDQQYTFFFHQARASGSFYEDDYNVKLSAALRVDTIH
                     VSGMDFIPGTDALLNGVIDVNTSTSNVSFNSVEIQIADVVLLAAGKVDYDKSRETVDI
                     TVKSDNTDLNEFISLMPQEVRKFSDGLEHRGTISIESHIKGKYSDGKMPSVDVSLLMQ
                     DGYIERPENNIALSNIRFNGKFTTPDFAAQEKYFLSMKNFSATLDGKTISGSLQIMNL
                     KNPQIKLSLKGDMSLGKIQEWGQWESVKEMAGNVKFDVQYEGTIDDFSNLQAKDFIDS
                     KSQGTLLIEDAKLQTEGMSKVAAVKKLDAIFSNRDLEIKEMNVAYGSSDFSGNAILKD
                     ILPFLFIDDNELKLSADLHSKNIDVTELFPEEENTQKTKETEIKFVLPKNVRAVVEYS
                     ADRLSYNKFGASNVKATVHVGENKVLAEDVSLKTLGGSIRGSGSCELKNKDVKVSLDA
                     NLKNLDVREAFIVFNNFGQKDLTYDNLRGRTDVSLQMKATFSETLEVDPASIVAGAEI
                     EIRDGQLVGYAPINDLEKIIKGRDFSDIQFEKMVSTISISDKTIKIPKTEIRSNVMNL
                     KVTGSHTFENNIEYHLEVKFSDVKKGGDKTRPEDEYGYVVDDGVGDPTIFILITGTVE
                     NPQYKQLDKKAMQEKVKQDLKAEKQNLKKILNEEFGLFKKDTSIKKVEPAPKENTKFQ
                     IEWEEE"
     gene            complement(50665..51726)
                     /gene="thiL"
                     /locus_tag="PKY63_00540"
     CDS             complement(50665..51726)
                     /gene="thiL"
                     /locus_tag="PKY63_00540"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPE99126.1"
                     /translation="MLEDKKPAHTDISTLGEFGLIHHLTKDFPLVNKSSRKGIGDDAA
                     VIEYDAEEQTLLSTDLLLEGVHFDLSWMPLRHLGYKSIVVNLSDIAAMNGVAQQVLVS
                     IGISNRFSVEALDELFAGMKMACDKYKVDLVGGDTSSSAQGLVISVTVVGKAKKEDIV
                     YRNGAQVNDLICVSGDLGAAYAGLLILQREKKTFEANPNFQPDLQGFEYVIERQLKPE
                     ARFDVMEKLRKVGIKPTSMMDISDGLSSELHHICSQSGTGCSIYEEKIPVDIETGRVA
                     EEFNVTDITMALHGGEDYELLFTVPLAEYEKLKDRDDILVIGHITDKNEGMNLITKAG
                     QSISLKAQGWDSFRSEKSM"
     gene            complement(52071..52664)
                     /locus_tag="PKY63_00545"
     CDS             complement(52071..52664)
                     /locus_tag="PKY63_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPE99127.1"
                     /translation="MKPVSATFFVQNYNRQLYLFLHDAATRLNTEIYFQRNSKHRLGT
                     FMVKGNDIPARVYVRRELPLGHFMIILCHELAHAMVWREYGHRAQPHGEEWKFAFKML
                     LRIVMSLYEFAPEWKTEALYQLEKPRVTYENSDAEIEIQEGETRVKDLPDGAEFIWKE
                     KTYRKLSLQRTRVLCQRLDDRKKYLFIGKAVVKTNNP"
     gene            complement(52685..54472)
                     /gene="lepA"
                     /locus_tag="PKY63_00550"
     CDS             complement(52685..54472)
                     /gene="lepA"
                     /locus_tag="PKY63_00550"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPE99128.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRLLEFTGTVSEREFQNQVLDDM
                     DLEREKGITIKSHAIQMRCERGGQKYKLNLIDTPGHVDFSYEVSRAIAACEGALLVID
                     ATQGIQAQTISNLYQAIDHNLEIIPILNKMDMASAMPEEVKDQIVELLGCPREDIIEA
                     SARTGMGIEEILDAIINKVPAPKGDPEAPLQALIFDSVYNSFRGIITYFRIKNGTVKT
                     NDLVKLISTGKQYRADEVGTLGMKLEPRSELSAGDVGYIISGIKVAREVRVGDTITKV
                     DNPCTTGIEGFKEAKPMVFAGLYPVDADDYEELRESIEKLQLNDASLTFEPESSAALG
                     FGFRCGFLGLLHMEIIQERLDREFNMDVITTVPNVSYKVYTLKKELVEVHNPSGLPDI
                     TKIDYIEEPYITANVITKSEYQGAIMKLCIDKRGVMKNQVFLSTDRVEVTFEMPLAEI
                     VFDFYDKLKSISKGYASFDYHECGFHEADLVRLDILLNGEQVDALSTLIHKDNSYSFG
                     RRMCEKLKDLIPRQQFDIAIQAAIGAKIIARETIKAYRKDVTAKCYGGDISRKRKLLE
                     KQKKGKRRMRQVGNIEVPQSAFLAVLKLD"
     gene            complement(54662..55384)
                     /locus_tag="PKY63_00555"
     CDS             complement(54662..55384)
                     /locus_tag="PKY63_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99129.1"
                     /translation="MKKRTAFLLSLLFFAGIGSHGQTALFTFGYSFEQYENTAPIDLI
                     VSDFNNHYSEPLFAPDNSLGSPGFSRGIILGCKVNNGWSSYGFDLHLHRFKSKGSGLD
                     SLDAEYFRKVKISHDGFSFFYNLNLIHGEGFRAGPGFSFNMDQFFVKYKIGNGPYDKY
                     LQAVDKFFLSGSVRFMLSFGKEDGFSFDLIPYYTLPFWNVNIAGYNNEINAGFANTYT
                     KQQMTFDLTNMGLIVSLNFFLK"
     gene            complement(55399..55863)
                     /locus_tag="PKY63_00560"
     CDS             complement(55399..55863)
                     /locus_tag="PKY63_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99130.1"
                     /translation="MESTDSKIPQEENTGSSKIWLIIGIILMIGLIGVLIYFNMTANQ
                     RHQKAMADMQLKYQQEITRLTMANRTIDSLSTIANHLIKYRGLVEAGYTRDSSRIVIP
                     HKIGDIVKLKFDSSDVVITDIIVGGGQFEYYVRYRVMHSNRKTEEIAPEMVF"
     gene            56401..57696
                     /locus_tag="PKY63_00565"
     CDS             56401..57696
                     /locus_tag="PKY63_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPE99131.1"
                     /translation="MNSFNNLPLLQQADLKGKVVLVRVDHNVVKKGLIHDPYRIDATL
                     GTLYYIVSRGGKLILMTHVGRPKDKKAGTINIGPESSVEPIVQYFENKLHVKFAIPEF
                     LPDGDKGYRGIETSVNHLIRDLREDRIDGIYLPNTRWFSGEEAKGEDADRFAYQLAGL
                     ADIFVNDAFGSWQPHASTVGITKYLPSYAGFLLQKEMQNLDRIFAPQKPFVAVVAGSK
                     FDTKIDSLFTLLEKSDYLVLGGVIYNAYLCAKYGFEIKGLEADDIKHANRFAEFAAKH
                     PGKLVELPFIVESDTLEGRKEGQFRTHDIRALKQGTKLNYVLDVARESFSEQSVLDIF
                     HGAHTIFVNAVMGFTPHFNEGTIALDELIDKNIDAVKLYGGGDTMQELKRLLPGLYIM
                     AIDNPKYYIFTGGGAVLKAIEQGTAFGLEPVKALMSGER"
     gene            57860..58717
                     /locus_tag="PKY63_00570"
     CDS             57860..58717
                     /locus_tag="PKY63_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99132.1"
                     /translation="MLLKKLTGFVLVLFMLVSCGMAQKQGVETFSIKIDSLYDDNNQL
                     NAAGNALVEKIAAKMNSFFSSCLNINHSCIHESDTICYQKLKTFVNAIIVKVPAGQFF
                     GPVSEPAHMSCEDCFELIISECPEISYDGNSPIIIIRDDEDTTYSPPVIIKESEDTVG
                     IKILDVLSKNRKIARLYKDTVMVKNNPYRIVYFIDTTEVHGINKNYSGDNFCVITQEK
                     DLPIYNRYQVFHVSYFRHGNYFADVSLLNRVRKKIIAVDCELKGDGADNWKVHRIKIK
                     KRNIYKIKR"
     gene            complement(58737..59720)
                     /locus_tag="PKY63_00575"
     CDS             complement(58737..59720)
                     /locus_tag="PKY63_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99133.1"
                     /translation="MSDQFSVQENFIRLLRNIVPSGVQLVAEVGTALSLSNDSVYRRL
                     RGETEFSLSEMVKLCNHFHISFDGFCESHRGSAYFQYQWPVDSIELFQQYLCRIRDEM
                     MILKNAKEREIMYVAADIPFFILFAFPQLAWFKCYYWMRSVMNVPEYQNSFFEDEVAP
                     EELMSIGNEILESYRIVPSTEIWSEQTLNSILKQIDFYSESGFFKDSETTQKLYADLL
                     SLMSEIRSMTEPGTKRKDHAGDKNYTFYRCDIEIGNNCIYTSIGKNKKVFLRHSTFNT
                     LETADEDFCAATRDWLTGLIRKSTQLSGMAEKQRNKFFQNLLAMIKNRMRV"
     gene            60118..67071
                     /locus_tag="PKY63_00580"
     CDS             60118..67071
                     /locus_tag="PKY63_00580"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPE99134.1"
                     /translation="MRNIYALLIFVVFLAFSSHTEAQTLKENTQQLQTEQTSNVIYPV
                     RAWVTKPLRELTQENPWREPDFTTIPWVSPDRKNRPRQTFIYSAADGPEYGNDSSIIQ
                     TKMGTRDATQSTKALIKNWAGIQSSSYPPDPTGAVGPNHYIQSVNATTVRIFDKNGNT
                     LESFLMGTIFGETSNAGDPIIMYDRFADRWFVSQFGDNNEIFIAISQTADPTGSYYAY
                     EYVSPQFPDYLKFSIWHDGYYMTSNQSTDKVYVFERDLMLTGNSGARAIYQTFTTGSV
                     SAFFVPLPADAADNHTLPAAGTPLPFFAYYDNAWGGGTDGIKIWTATTNWSAGTLTIS
                     TTPTQVNTSAFDASYDSNWDDVPQPNGVELDGIGGVLMYRAPWRSWSGYNNVILTWGV
                     KISESPRQRAIMWCELRQISGTWSVYQQGIYAPDTHTRWMSSATMDDNGSIGLCYAKS
                     STTIYPGLYYTGRLASDPLGTMTLTETQVVAGTGSQSTYNRFGDYSQTSLDPDGVTFW
                     HTGEYVTTTSGQETRVYSFQIPAGPLAPVANFTADATAPFCSGTVQFTDMSSNAPTSW
                     LWNFGDGQTSTQQNPSHTYTASGTYTVTLTSTNTLGNDSEVKTSYITIDIPATASATN
                     AERCGTGTVTLSASGTNTLNWYDAASGGNLLGTGTSFTTPSISTTTTYYVENNIPSAP
                     ANVGPAVGGASRNIAAILYFDVYQPMTLISVQARAQTTGVKTITLLDNTGATVYTANV
                     TVNNSVTTITLNWVIQPGSGYQLLTLENSALYRISSGVSYPYTAPGLCSITGCDIGSA
                     YYYSWFNWNVQGLGCGSTRVPAVATINPTVTPSVAVTPSATNICSGTGVTFTATPSNG
                     GTPSYQWKVNGNNVGSNSPTFTSSALSDGDVITCTMTSTESCASPATATSSGITMTVN
                     PVVTPAVSIAASASTICDGNSVTFTATPSNGGTPTYQWKLNGNNVGTGGTTYTNSGLN
                     DGDIVSCVMTSTEVCATTGTANSNPVTMVVNPNVIPSVAVSESQNNICDGTNVTFTAS
                     PSNGGTPAYQWYLNSNPVGSNSATYSNAALNDGDEITCVMTSTAACASPSSVSSTPVT
                     MTVNSAVTPSVSVNASSTSICDGTNVTFTASPVNGGTPTYQWLLNGNNVGSGGTTYSN
                     ASLNDGDVITCIMTSTETCATPTSVTSLPVSMSVSAPVTPSVAVSASETNICSGTSVT
                     FNATPTNGGTPTYQWQVNGSNVGTGASYTNASLNDGDEITCIMTSTAACATPETVTSA
                     PVNMTVNTTLTPSVMVSASATTICSGSSVTFSATPTNGGTPSYQWYLNGSPTGINSAT
                     YSNNALNDGDVITCEMTSSEICASPVLVVSSPVTITVSSSVTPSVSVNASSTNICSGT
                     AVTFTATPTNGGTPAYQWYLNGSPTGINSATYNNAALNNGDEIICVMTSTESCASPTF
                     ATSAPVTMTVSSTLTPAVSVAASATSICSGNNVTFTANPVNGGTPAYQWYLNGSPVGG
                     NSATYNDAALNDGDEITCVMTSTELCASPATATSAPVIITVNSTLTSAVSVAASATSI
                     CSGTDVAFTANPVNGGTPTYQWYLNGSPVGGNSATYNNSGLNNGDEITCVMTSTETCA
                     SPTTATSAPVTMIVNTSVTPSVAINASANNICSGTSVTFTATPTNGGTPVYQWYLNSN
                     PVGSSSATYSNASLADGDVVSCMITSSETCATPSTATSAPVVMTVNANVTPSVSITPS
                     ATNICSGSEITFTANPVNGGTPSYQWLLNGSVTGTNSPVYSSSTLNNGDIVTCTMTST
                     ETCADPVTAVSSQVAITVSTSVTPSVAINASATSICEGTSVLFTATPVNGGTPAYQWK
                     INGNNVGGNSATFNSTSLNNGDVITCEMTSTETCANPASVTSGSITMTVVPSVTPLVS
                     IAASTNDICYGTPVTFTATPTNGGTPTYQWILNGANVGSNSPNYSSSMLQNGDIISCI
                     MISNVSCATATTVNSNDLTMIVNPSVSPSVSIVSSENGVCEGTPITFTAAPVNGGTPS
                     YQWLVNGANVGSNMSTFTSTTLIDGDVVSCVISSTAPCVSPVMATSNTIVMNLYPNLT
                     PSVDIASQPAGTICPGTTKEFTAVATNGGTAPTFEWFVDGVSVGTGSVLNGVYSNGQV
                     ISCTMTSTQPCVMPAIVDANPITVSTFNVTPVTISDNGGTLTSSAAYGNQWYEVSSGV
                     VVGAIGQTFAPLYEGVYYVVVTDANGCTTTSNQIEFILESIDENGAATVTFFPNPTSG
                     NLAITFNTAFNNGTLKIENAIGELVFEKNITQSAGSVFTIDFSAYAPGSYFITVKDSE
                     NEIKKQVIYEK"
     gene            67246..68451
                     /locus_tag="PKY63_00585"
     CDS             67246..68451
                     /locus_tag="PKY63_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPE99135.1"
                     /translation="MKKIFFPFMLITLVLAFASCQKDKEGDNTDLNFGYVKGNVYTPS
                     GVPVPAASIFIDVEGEIYFARTDKNGYFYLRAPEGERVLNLQGGDGSIFRSQYNVEIK
                     ADAVTAFPDNTLKISQAEELAYIPGAFDHIETILIDSLGYTADMLSVADLDNLTLLES
                     YGAIFLNCGKTGNMDSVKYENLEQYVMNGGNIYASDWAVEYLTGDGNFLANSSQPFVM
                     HEHKLTHASNPKSGCLSPEVGGFIDDATLCTDKDGPSTMLYQADIVDTLIQAFLGKTK
                     IDIEYDLGAWEVIKDLTIPWEVLIQDPVTYGPLAVRMNFPTQAATRSQLDQGWVTICH
                     FPPGNPSNMQTITISINALPAHLAHGDYIGSCVGGSGTIFFTTFHNHVQGNIGPDTYQ
                     MLQYFIMNL"
     gene            complement(68802..70181)
                     /gene="hisS"
                     /locus_tag="PKY63_00590"
     CDS             complement(68802..70181)
                     /gene="hisS"
                     /locus_tag="PKY63_00590"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPE99136.1"
                     /translation="MAKTVLSVPKGTRDFSPAQMARRNYIFSILRSVFEKHGFQPIET
                     PSMEQLSTLMGKYGEEGDRLIFRVLNSGDFMNSVPAEKEHTSSEVIKHISEKGLRYDL
                     TVPFARFVVMNQNEISFPFRRYQMQPVWRADRPQKGRYREFYQCDVDVIGTRSQMEEA
                     GLMDILDTAFQKLGVNVMLKINNRKILQGIADALGVSDRFVEFTVSLDKLDKTGWEGV
                     GKELIERGFPTDVPQKLMDIMQPVSSNEAMLTQLKKYLVSEAGMKGIAEMEELLGFID
                     ALELRSQVKFDVSLARGLSYYTGAIFEVSACDVAMGSICGGGRYDDLTGIFGLPGVSG
                     VGVSFGADRIYDVMMELNLFPENISRTADILLINFSEDSAADLMKMAKRLRENGIACV
                     VYPEAAKMKKQFSYADAYAFPYTLIQGPDEQAAGIVTLKQMSSGKQVQVSETALFSFS
                     LNDMSALFE"
     gene            complement(70678..72147)
                     /locus_tag="PKY63_00595"
     CDS             complement(70678..72147)
                     /locus_tag="PKY63_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPE99137.1"
                     /translation="MAGQTAVYGISSIVGRLLNYFLVPMYSRVFLPEESAVYVEMYAY
                     VAFFIVILTYGMETAFFNFNQKEENKNRVYSTTITSLFTTSALFIAAALIFAQPLAAM
                     LRYGDHPEYIQYFALIVGLDAFTSIPFARLRAENKAMRFAIIKLIGIFVNIGLNLILL
                     LLVPYLYKNYSWGPEMLSWICDGEPVVGHIFVSNLVASVVVFILLIPDIFKIKFGISF
                     SLLKRMLSYAAPLLILGLAGIANETVDRILLKYLLPEDVAMYQLGIYGMCFKIAIVMS
                     IFVQAFRYAAEPFFFARQKDADAKQTYADVMKYFVIIMTAMFLAIILYMDIVKYFVGE
                     EYYSGLKIVPILLYSHVFLGIFYNLSIWYKLTGQTRFGAYISLIGTAISVGLNIALIP
                     VIGYMGSAWANFACYAVMMVISYFLGQKYYRVNYDLKRILSYMMISAGLYFVSVYTTQ
                     HLFADNVAQRLAFNTLLLMVFVIYISFSEPRLKRIFVRK"
     gene            complement(72464..73327)
                     /locus_tag="PKY63_00600"
     CDS             complement(72464..73327)
                     /locus_tag="PKY63_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPE99138.1"
                     /translation="MRQLKISKSITNRETASLDKYLQEIGREELITAEEEVQLAQRIK
                     QGDQIALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKEASRLEQRYERPPSAEEIAT
                     SLDLPEDKVMETMRLSGRHVSVDAPIVSDDTTSMLDVMVNYDAPRADQNLISESLSRE
                     IERSLATLSEKERDIINLYFGIGVNHSWTLEEIGVHFDLTRERVRQIKEKALRRLKHN
                     SKSKLLKTYLG"
     gene            complement(73443..74837)
                     /locus_tag="PKY63_00605"
     CDS             complement(73443..74837)
                     /locus_tag="PKY63_00605"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPE99139.1"
                     /translation="MKKREIVFRMMAGVFFLIFSTEIMLAEPMMKPVPGDPVDFTMAA
                     DKSVPAVVHIKTSFTRKNAYYDDFFAPFYDFFNVQPFSQQYPINATGSGVIVSDDGYI
                     VTNNHVVQDAEQISVTLNDKREFVATVVGLDPSTDLALIKIDEKNLPFLIFGNSDQVK
                     IGEWVLAVGNPFNLTNTVTAGIVSAKARNINILGGGSSVESFIQTDAAVNPGNSGGAL
                     VNTKGELIGINAAIASNTGSYSGYSFAIPSNIASKVVSDLKEYGMVQRAYLGADIVDI
                     TAATAKQYGLSSMKGVYVSAVSDGGAAIEAGIEAGDVILSVGGEEVNSSSRLLELIAE
                     KHPGDKLLLEIERGNKTISKPVTLLNRRGEKVLLKKEDPALSTFMGATFSEPDQQTMS
                     RLRLINGMQISELNEGALKNAGIKESFIVTKIDQTIIYSVEDIENALSGKSGGVLIEG
                     VYPNGMKAYYGFGL"
     gene            75025..75762
                     /locus_tag="PKY63_00610"
     CDS             75025..75762
                     /locus_tag="PKY63_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99140.1"
                     /translation="MTGDSSKHHRKHLNRSFITGMIMGAALLLGAYFTYLLITNDEQE
                     SISRKTATVKNDSIPPLYNLTGFPRILFDSSLQNIDSSYYINKWNQLIEMNGYREMDS
                     LFIDSVIRKMYFDSIRAVSDVSADTIILQNDRLIATKNINVRIQKKLLLNEDTTGSDY
                     IYSDAKFIIEFWESPIKFRGFKRTTNYVVLFGLNPEKTTTFLQIDNILYIREKQIWYA
                     IPCSPEFSGLAPVTSPQLLSKINAANK"
     gene            75773..76540
                     /gene="dapF"
                     /locus_tag="PKY63_00615"
     CDS             75773..76540
                     /gene="dapF"
                     /locus_tag="PKY63_00615"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPE99141.1"
                     /translation="MNTAYKFHGAGNDFILIDCFEKKELFSEKEIEALCNRHTGIGAD
                     GLILIEKTNKASFKMIYFNSDGKEAEMCGNGARCAFAFAFSKKYCTINDSFEASDGLH
                     MGSIIQNNTHDWIVRITLNVNIEPREIEDGSFFANTGVPHNVRITKNVSGIDVKTEGL
                     KLRYNKKLFPDGANINFVEIKDNELYIRTYERGVEEETLACGTGITASALVANAFFGL
                     KFPITIHAIGGLLRVEKQNGWLWLEGPASHVFTSNYF"
     gene            76492..77076
                     /locus_tag="PKY63_00620"
     CDS             76492..77076
                     /locus_tag="PKY63_00620"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPE99142.1"
                     /translation="MARRPSQSCIHIKLFLIFMLNGSVAFLRTPDISDVQFMLDIENN
                     ERYWHLSGVTSPYSKKDIEQFINESSSNLKTDCQLRLVIIDISTKKRAGLLDVFEYEP
                     LHKRAGIGIFLLEEFRGHGLASDALRVLIQYLFKIMKLHQLWCNILTENTESMKLFNG
                     AGFNQTAIKKDWIFHNGKFHDEAIFQLINPNHRV"
     gene            77073..78122
                     /gene="mltG"
                     /locus_tag="PKY63_00625"
     CDS             77073..78122
                     /gene="mltG"
                     /locus_tag="PKY63_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPE99143.1"
                     /translation="MKKKLIFIVLALLMVVGITGAWYVYTKAFSDNINLKAKTKKIIF
                     LPTGGNIQNLYDTLKKHEILKSFSSFKMLAEYKKLDKKYKPGRYQIAAGMSNNALVNM
                     FASGRQTPVKITFNNIRTRKDLAERIGTQLECGEEALLKILNDEKTAEKYGLNKDNFL
                     VLFIPNTYEFYWNTSADQFIDRMHSEWKKFWTAERLQKAENIGLTPAEVSVLASIVKG
                     ETNKSDEMDKIAGVYINRLKDGWKLQADPTVVFGIGDFTITRVLKKHLEYDSPYNTYM
                     YEGLPPGPINLPDATVIDKVLNYTHHNYMYFCAREDFSGYHNFAVTSSQHSANARKYQ
                     QALNAWEKKKKDSGK"
     gene            complement(78156..78860)
                     /gene="rnc"
                     /locus_tag="PKY63_00630"
     CDS             complement(78156..78860)
                     /gene="rnc"
                     /locus_tag="PKY63_00630"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPE99144.1"
                     /translation="MGIFRKKHADELSKSIKNIFGFRPGNIFLYELAFMHKSAHVERN
                     GHEISNERLEYLGDAVISVIVADYLFKKFPFADEGVLTEMRSRVVSRNSLNKIAEKLG
                     VGRLVTMSRVPANNAYTGNALEAFFGAVYLDKGYGFAQNLLVSFIFGRRIDADAIIST
                     ETNFKSKLIEFCQKERLNLEFSIVDEIQTGRKRQYVCEARIDGEARSRGIDYSIKGAE
                     QNAAEKALESLRNGAE"
     gene            complement(78860..80110)
                     /gene="fabF"
                     /locus_tag="PKY63_00635"
     CDS             complement(78860..80110)
                     /gene="fabF"
                     /locus_tag="PKY63_00635"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPE99145.1"
                     /translation="MNKRRVVVTGLGAITPIGNTVPEFWDSLLNGVSGCGTITRFDAS
                     KFKTQIACEVKNYDSTQFFDRKEVRKYDLVSQFGIISANEAIKDSGLNLEQEDCSRIG
                     VVWASGIGGLITFYEEVSEFAKGDGTPRFNPFFIPKMIADITAGHISMINGLKGPNYC
                     TVSACASSANAIADAIMLVRHGYADAMVAGGSEAAINQGGIGGFNAMHAISTRNDDPK
                     TASRPFDKDRDGFVLGEGGAGLVLEEYEHAVKRGAKIYAEVVGFGLSADAYHLTAPHP
                     EGEGARTAMQTAIKDAGITPNDVDHINTHGTSTPVGDIAEPKAIVSLFGEHAPKISIN
                     STKSMHGHLLGAAGAIEAIAVVLSLKNGIIPATINHFTDDPEIDSSLDFTYDKVKERN
                     ITYALSNTFGFGGHNVCLAFKKFE"
     gene            complement(80123..80359)
                     /locus_tag="PKY63_00640"
     CDS             complement(80123..80359)
                     /locus_tag="PKY63_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPE99146.1"
                     /translation="MSEIAEKVKAIIVDKLGVDAAEVKPEASFTNDLGADSLDQVELI
                     MEFEKAFELQIPDDQAEKIGTVGEAIKYIEENKK"
     gene            complement(80562..83831)
                     /locus_tag="PKY63_00645"
     CDS             complement(80562..83831)
                     /locus_tag="PKY63_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HPE99147.1"
                     /translation="MKVHILPLLLSFCFAFSGLSLKAQNESKSRPNNSESGFVYASSF
                     AVTRPLSELIDEQPYVEPDFTTIPWVSNDRKNRPAQTFVYSAEDGPEYGNDPAIVQTS
                     MGKRTVGTKATLQNWAGVTSSAYPPDPTGAVGPNHYVQMVNATTVRIFNKTGVEQESF
                     QLGTLWGSSSNAGDPIVMYDKFADRWFLAQFGSSTQIYIAVSQTADPTGSYYTWTFNS
                     TEFPDYLKFSIWQDGYYMTANQSSDKIHVFERSAMLTGSSSARMTYSNFSTGSVSSFF
                     IPLPADAADDDALPASGTPLPFFSYFDNAWGTGNDAVKIWTATTSWGTTPSVTVSGPT
                     SVNTNSFDASYQSSWNDVPQPNGQYLDGIGGVLMYRAPWRSWSGYNSVVLTWGVLISN
                     SPRVRAIYWCELRQTSGTWSMYQQGIYQPDSDTRWMSSAAMDDNGSIALCYAKSSTTT
                     NPGLYYTGRLASDPLGTMTFAESTAIAGNASQSGVNRFGDYSQTSLDPDGVTFWHTGQ
                     YCASTSGQETRIYSFQLPAGTPTDVSNFDATAVSSSEIELSWLLNSNSNPVLVAWNTT
                     NSFGTPVDGTAYVAGNTITGGGNVIYYGSATTFNHTGLNPSTTYYYKAWTNTGSYTWS
                     TGVTDNATTFATGGSTYPFTLDFESSTDYTTDFAPWTTADQDGLATYSSGDATFTGEG
                     TAFAYMCMNPSLSGWTAAQGDVAHGGSRCGMAICPSDQSTSDDWFISPAMALGTNSSF
                     SLWVLSPKPSTWGNNSYQIMISTTDNQPASFTALSAVVEAPATWTQHTYSLSAYDNQT
                     IYLAIRHVSTDMFMFWMDDLEFNSTVAAGTAAAVVTDPVSTSACPGTSVSFTVTASGD
                     PTITYQWQKNGVNIAGATMSTYTIASVSASDAATYRCLVSNAFGSELSSGAVLMVNAA
                     TLITSQPASVNVFEGDDVSLTVGASGGSLTYQWKKNNVNISNGGNISGATSATLQILN
                     VTSADFGTYSCVVTGTCGSVTSNGAVLTVLTGVDESGSIKPDIFPNPSSGIFNIQLGD
                     TPASGEYYVYDTEGKLILWKKYSEQRDISIDLHGRAQGAYLFRIDANGKTFFNRLILK
                     N"
     gene            84034..85539
                     /locus_tag="PKY63_00650"
     CDS             84034..85539
                     /locus_tag="PKY63_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE99148.1"
                     /translation="MDIYRRKRFWKWGLFIIGLLIVNASVMFSNNLVKKIATDERKKV
                     KIWANAIQQKANLVNYTNDFFKKIKEEERKRVEIWAEATKRLINAGINEDLSFYTQII
                     AGNTTIPVVLTDEEKNIIAAKNVDFSTDTVNKLEGKRLNEFSKIEPIVVSYGEFVNYL
                     YFKESLLFSELRNVLNDLTESFFSEVVINSASVPVLILDSARSKVYSSGNINQEILKN
                     PDELKELIKTMETQNPPIIIDFEGQGKKYIYYKDSYLLTQLRYYPFVQLFVVGLFLLA
                     VYILFNTTRKAEQNKVWVGMAKETAHQLGTPISSMLAWLDFIETDEKNKVYIDELRND
                     IRRLQDVSARFSNIGSAPDLPMTLLSESVYNSVRYIRARSSKKVSINILFRENDQLML
                     PLNENLFGWVMENLLKNAIDAMEGEGSITISLHNASRHIMLDVTDTGKGFPKSQYKSV
                     FRPGFTTKKRGWGLGLTLSKRIIEEYHKGKLFVHSSVPGKGTTFRIVLPLK"
     gene            85545..85943
                     /locus_tag="PKY63_00655"
     CDS             85545..85943
                     /locus_tag="PKY63_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HPE99149.1"
                     /translation="MKKPLAFLLIIVMAASCRFLGMEKKNSPEYVAKKFLTHFQKLEF
                     DEASQYGTENTKMILRLFKSLSAMVSDSTKSQNNFSDAVIQKCTVEKNTALCRYTANG
                     KEETIDLIRQDGKWLVDLKKEQKNPFNPGK"
     gene            complement(85953..86780)
                     /locus_tag="PKY63_00660"
     CDS             complement(85953..86780)
                     /locus_tag="PKY63_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPE99150.1"
                     /translation="MEKAKILYVSQEITPYLRDSQMGNVCRHLPQGTQEKGKEIRTFM
                     PRFGCINERRHQLHEVIRLSGMNIIINDSDHPLIIKVASIQQARMQVYFIDSEDYFHR
                     KFILHDKDNRFYNDNDARSVFFNRGVLETVKKLGWAPDIIHCHGWMSALAAAYVKKVY
                     KKHPIFSESKIVISLYDDHFSEYFSEQVIANIVMDDLTAVGLPQLKKDRNYVGWMKTA
                     IDYADGVIVGSENVNPDVLEYAKKSKLPMLEYQGEENYIDAFNKFYDRVLINEEVAC"
     gene            86878..87717
                     /gene="panC"
                     /locus_tag="PKY63_00665"
     CDS             86878..87717
                     /gene="panC"
                     /locus_tag="PKY63_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPE99151.1"
                     /translation="MNIFRTIKEYKDWQESLTDKTSVGFVPTMGFLHQGHISLVSASM
                     AENDVTIVSIFVNPTQFNNPEDLEKYPRNEERDLQMLEMAGTTAVLIPSVSEMYPEPV
                     TRTWTFPGLDDVMEGAKRPGHFNGVAMVVSELFRIIQPNIAYFGKKDYQQLMIIRQLA
                     ANDFPQITIKGMPIVREVDGLALSSRNSRLTPEEREMAPLIFEVLKRARQLIHEFSPE
                     DVCIHCERMLAGSGMIQPEYFIIADAQTLKPLSKKEDATHATAFVAAWLGKVRLIDNF
                     ELF"
     gene            87730..88074
                     /locus_tag="PKY63_00670"
     CDS             87730..88074
                     /locus_tag="PKY63_00670"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPE99152.1"
                     /translation="MNIQVLKSKIHKATVTEADLYYTGSITIDENLMDAADLIEFEKV
                     HVLNINNGERLETYVIKGARDSGTICMNGPAARKVAPGDTVIIVSYCTLEYNEAKKFK
                     PSIIFPGKNNKL"
     gene            88078..89100
                     /locus_tag="PKY63_00675"
     CDS             88078..89100
                     /locus_tag="PKY63_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPE99153.1"
                     /translation="MKKKIFKIVQTLAFIGLGIFFIFFFWGKLDTEQQEKVIDNFLKA
                     NYFWVLLALSAGILSHILRAARWNLLIDTIEKAPPLRQSFWSLMAGYLANLAVPRLGE
                     ITRAILLSKQSKISFDKLFGTVVAERAFDFFFYLALFFAAIAIFWDNVKTYVTERIMG
                     GFAEKTSVFSGWKIWLILGILCVLFLLFLWILKRFRHKPLIQKINRIIQNLLSGLLSI
                     FKLRKTGLFFVYTILIWVMYWLMVYFMYFSIPSTSGLSAESAFMVLVFGTIGIIVIQG
                     GIGIYPLIVSEVLMIYGADITDGFTIGWLSWTVQTIMILVMGLIAFTLFGFQKNKKDG
                     LQKTDQ"
     gene            89075..89566
                     /gene="rfaE2"
                     /locus_tag="PKY63_00680"
     CDS             89075..89566
                     /gene="rfaE2"
                     /locus_tag="PKY63_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPE99154.1"
                     /translation="MDCRKLISEKIMSREQLIQRVNTWRTERQTIVFSNGCFDIIHPG
                     HAQYLAAAAALGSRLVIGVNTDDSVKRLKGAARPVISENERCLLLASFQFVDAVCLFD
                     EDTPLELIEAIIPDVLVKGKDYTIDQIVGADVVLQHGGKVETIELVEGFSTSSLIEKI
                     KRI"
BASE COUNT        26771 a        20482 c        18106 g        24282 t
ORIGIN      
        1 ccatgttctt cgtggatttt atactgcagg gcagtacttc gaactatgga atgttagaca
       61 acactagtcg attactggca ttgttaagga tgatcatact gaaatttatt tctttgacaa
      121 aagtaacacg accttcaata gtccgaaata caatggagga catggaagag tcggttgcag
      181 agatctctat ccaactattc catgttcttc gtggatttta tactgcaggg cagtacttcg
      241 aactatggaa tgttagacaa cactagtcga ttactggcat tgttaaggat gatcataaaa
      301 aatattgcat tgaaaaacag ctacagtcgt tactttataa cgcttaacac atgactttaa
      361 aaaggcaatt cggaaagaaa ctgtcgaagg ccatagacaa aaacatcttc ccgtaccctg
      421 aatttttcgc cttcagggat aatgataaaa ttcttcagag tgccaagatc tgcaatcgct
      481 gttgcaaagc cgcgtgtcat tgcgggagac gaagaaaatt ttatttcaat gctaaccatg
      541 ggtttcgatc ctttcccaag aacaagatcg gtttcaacac cggtttgtgt gcggtagaac
      601 caaatattcc agtcgttttt taatagcggc agcagctgat ttaatacaaa gttttcccag
      661 gaatgacctg ccatcgcatt acccataagc tgatcatagg aactaagacg taacaagcgg
      721 tgtaaaagtc ctgtatcagc aataaagatt tttttagact tcacgattct tttgccgata
      781 ttggcatcga aaggttcaat gcgatgaatc ataaaagccc cttcaagata atcgaggtaa
      841 ttatttacag tatggtgtga aacgccgaga gagcggccaa gatttgagga attaagaagg
      901 cttccggcct gccacgcaag catttcccac aatcggcgaa tttgcacagg atcagctttc
      961 agaccgagca taggcaaatc gcgttcgaga taggtgtaaa taaaattatc gagccattcc
     1021 tttctgaatc tctcatcatt atcgagcaca acatcgggaa atccgcccca aaacatcaaa
     1081 tccttccaca acgcaggatt attcaattcg ctcagaatga aaggatgcaa atggatatag
     1141 cggactctgc cagcaagtgt ttcagatgtg tttttaataa tatcggggct ggccgatcca
     1201 agaatcagaa agcggccgga gcgtttgaat gaatcaattt cggctctgat ttcagcaaaa
     1261 agctcaggtt ttcgttgaat ttcgtccaaa acaataagtt tgtccctgtt atcacgaaaa
     1321 aaaaggcctg catctttaat gcgcatcaga tcttcatatt tctcaagatc gagatagatg
     1381 aaatctttgg cagtggcaga acacaattgt tttgcaagcg ttgtctttcc acattggcgt
     1441 ggtcccgtga tgcagacggc cggaaacttt ttcatggacc aggttagctc tggatataaa
     1501 aaccgtgtaa tcatggattc attttctggt agtaactact tgtattttac aaaggtaata
     1561 aataacgttg tattttggaa tttttgtttc catatttcaa ccttttttag taattatcgt
     1621 tagatgttac agggtaacag ggtgacaagg gcggcagcac aaagcacagg gcacaacgcc
     1681 cgggcttatg gcttaaggct taaagaatat ttagaatcac gaatttttaa gcattaaatt
     1741 ttgaagcatt aagctttgcg ttgtgcgatg cacaaagccg gcagttccat tgtcattttt
     1801 caaattccgg aaaattagta ccagaaataa taaaaatatt tatacctttg gaaaggatga
     1861 attcattcta aagctacaaa gtcatccagc cacaaaaaaa gaacgcgaca acaaaatgcc
     1921 cttctatgaa accaggctca aaattcaaca ttgacgattt catcaaggaa aatatcaatt
     1981 cttccgacgt gctttcgcaa tttaaaggaa ctatttccga aaaaaaatca gaccggattc
     2041 ttgacaatat ggaggggttg ttcggagagc aggaaacccg catccgaaaa cgcattttgc
     2101 ttgctgcaag cgaaattctg caaaatgttt ccatgcacaa tgaatatccc ttatacccgg
     2161 ctgtttttct ggcaacacgc acccggcaag ggttcaagat agactgcgga aatgccgtta
     2221 gtatcacaag gcgtacagtg gttgaaaaac gcatccgcga agccaaccgc aaagcaggcg
     2281 gcagcatgaa agacaatgtg aggcaggccg aagccgaaaa accgaccatc gccgatattt
     2341 caaacggcag cagcggactc ggattacttg aaatccgccg caaagctggc atgccgctcg
     2401 attttgcttt caccattata gataatgaaa cgtttttctt tcatctgatt gtgaagttca
     2461 atatgggaga gtagaactct gaacaaggaa cctgtctgca acaggcaggc aaggaaggca
     2521 gaaggcagaa ggaattcttg attaatgaac tctgaacaat ctgtgcattc tgacggatca
     2581 agagggacaa ggaaatgtga aggaagattc gacttgttga atatttcagg tgatactata
     2641 ataaaatttt caactgcaga aatgaaaagg gtttttaatt tagaggatag actagctgtt
     2701 tatagtgcaa ctgtcctaaa actatgcaac ttgcttccta aaacttatgc tgctgatgtt
     2761 attgccaaac aactaacgag agcgggcgtt tctgtcgctt taaactatgg tgaagctcaa
     2821 agtgcagaat cgagaaaaga ctttatccat aaagtaaaaa taagtcttaa agaggccaga
     2881 gagtcctatg tttgtttaaa aatcatcaat ctatctgaca ttacagacca gaacgatcta
     2941 ttcagcagcc tgcttaagga agcaaatgaa ataatctcaa tactcgtaaa atccgttgaa
     3001 actgccaaaa agaatatgga aaacaataag gaataaatat ctcccattta caataattaa
     3061 tacaatttca ttattattcc ctcatccttt ctcctaatta aatattcatc ggcatcactt
     3121 ctaacttctt acatccttgt tccttgttca ttattcatta cctttgcagc aaagttgatt
     3181 tatgaaaatt ctcatcacag gttcgggcgg acgtgagcat gcgctggcat ggcgactggc
     3241 acgcgatatc ggaaaggaaa atattttcat tgcacccgga aatgcgggca cagtctcttg
     3301 cggcaccaat ctcccctgct catacaatga ctttgaaaag ctgcaggaat acatcatttc
     3361 cctctccatc gacatggtgt tggttggtcc cgaagagccg ctggtaaacg ggtttaccga
     3421 tttactgaaa aaagatccgc gcttcagtaa actcatgatt atcgggcctt cggctcaagg
     3481 agccatgctc gaaggcagca aggattttgc aaaacaattc atgatgcgtc atggcatacc
     3541 cacagcggca tacgctactt tcactgctga gaagaaagcc gaagcacaat cgtatatccg
     3601 cagccacagc ggcccctatg ttatgaaagc cgatggactt gccgccggaa aaggcgttgt
     3661 aattaccgaa tcagccgatg aagccgtaaa acacattgca tccgttttcg aagacaatat
     3721 ttttggcgaa gccggaaaaa cattagtcat agaacaattt ttagaaggaa ttgaaatgtc
     3781 ggtttttgcc ttgacggatg ggaaaaacta tgtattgttg cctggcgcca aagattacaa
     3841 gcgcatcggc gatggcaaca caggccccaa tacaggcggc atgggaacag tttctccagt
     3901 gccttttgcc acggaagaat tcatgcataa agtggtaaca cgcattgtag agcccaccat
     3961 cagcggatta cagaaagacg agattgatta ttgcggattt attttcttcg gactgatgaa
     4021 tgtaaaaggc gacccatatg tgattgaata caatgtacgc atgggcgatc ccgagaccca
     4081 ggttgtgatg ccgcgcatcg gcggatcttt tgcagacatg ctgaaagccg cagcaacagg
     4141 cactttgact gatgtaaaaa cagaaacatt accacagacc acattatcca ttgtacttgc
     4201 atcggaaggc tatccgggca gttaccaaaa aggcaaaacc atttcacttc ccgaaggcaa
     4261 tcatttgatt tttcatgcag gaacaacaca aagcaacaat caaattacaa catccggggg
     4321 gcgagtattg gctgttgtcg gcactggaaa caccatcagc gaagcacgca actcggctta
     4381 cacactggca gaaaaagtac attttgaagg aaaaacaaac cgcagcgata tcggactcga
     4441 tctgatgcaa tatgaaaaca cttaatgccg aacgtaccat caattcgcag ctgaaattta
     4501 ttttcatcct gttttggctt gcaactcttt ttctaatgct ccaaagaggc gaggccacta
     4561 ccatcatccg gctgcatcca tggcttggtg gcatcacaga aaccacactg gatctcaatt
     4621 tcagttggat tatcattctc cgggttatat tcattgctgt atcaacaatt cttttttggc
     4681 tgatttccat ccgaaatctc gaattgccaa aaaaagacta tatggtgttt tttattctgc
     4741 cggtcattca ttttacgatt actgactggg caatgggact catggtatct gtttactgga
     4801 ccacttttct gtttattctg tttcagttat tacccggcaa caattcaaga gctgtacttc
     4861 gaaaaacttt aacagcaggc gttttcggcg gcttactact tctcaacggc gcctttgccc
     4921 tgctgttttt tcttgccgga ataataacaa tgattatcag ccagaacatt tcattccgta
     4981 gcacaattgt atggataacc ggatttctga ttcctggatt tttccttttt ctttggtatt
     5041 tcgtgttcga taaaaccaat attatttttg gcaattctga cgcgttcatg ctcgaagaag
     5101 aaatgctgaa atttgcattc ccacttactt tctacggatg tccggctctt ttgattattc
     5161 tttcattgtt tgctcattca agaatgagtg aatcgaaaat ttcagttcga agaagctatt
     5221 ccgccatttt gttttcattg attgcacttg ctcccgccat gatttcatca ctattcaata
     5281 cttatatttt attttcaatt tacggtctgg caactgtatt ttactggact cgtctgatgt
     5341 acaacacccg acggaaagcc agcttcatca tactctggct catgcctttt gcattccctg
     5401 tattgtatta ttatcttctg aattacaaat aaacgacatg gcggccttgc tacatcattt
     5461 ttcagaatcg gcttccgagg ctggttgcga cgaagccggg cgcggatgtc tggctggacc
     5521 ggtaacagca gcagccgtaa tattaccgca aaattttttt catcctttgc tcgatgattc
     5581 aaaaaaactg aatgaaaaag aacgcaatct gctcagagaa atcatcgaag aacaagcact
     5641 tgactgggct gtagcatttg tcgatcatgt ggaaattgat aaaataaata ttctcaatgc
     5701 ttccattatg gccatgcatc tggctttgag taaactaaag acaaaacctg cacatattat
     5761 tgttgacggc aacagattca aaccatttaa aaaaattccg catacaacca ttgtgaaagg
     5821 cgatgggaaa tacatgagta ttgccgctgc aagtattctt gcgaaaacgc accgcgacga
     5881 attcatggag caactgcata aagaatttcc acaatattca tggaacgaaa acaaaggata
     5941 tccatcgccc ggacacaaaa aagccatagc attacatggc attacgccat accaccgcaa
     6001 gagtttcaga ttgaatgaac aattgaaaat gaaattttga accggtatgg tttatgatag
     6061 cgcatgcata aaaaaatact aactttgcaa aaaatcaaaa catgcgtttc ctgattttta
     6121 ttttaataac aatgtcatgc acagcttttg ccagtgcaca aggccgggtt aatattctcg
     6181 gagataaagg cgtagaaaca cttgtagcca aacacatttc gttcaatgaa aatgtaaaag
     6241 gatttcccgg ttacagaatt cagatctatt ttgaatccgg caattactca aaaaacaagg
     6301 cctttggcga aaaaggcaaa tttatggccc gctttcccga aataaaggcc tatgttgtgt
     6361 ttcaggaacc ttattataaa gtcagagttg gcaacttcag aaacaaactc gatgctgaag
     6421 cattcaaaca acgcataaaa gaacaatggc ccgaagcata tatcatcaaa gacgatatcg
     6481 aacttcctga aatttttgca ccaaaggaaa ccgactaagc ccataccatc aaaaaaccgc
     6541 attcaatgcg gcacacaaga caaagcatga attttccgga actcgacatc agggattaca
     6601 attatcattt acctgaagaa aacatcgctc aatttccccc cgaaaaacga ggtggctcaa
     6661 aaatacttct ggcgcatcag caaccgtttt cgattgttgg attcaatgaa ttgaaacacc
     6721 atatcccaaa aaacgcactt ctcattttca atgaaacaaa agtcattcct gcacggctta
     6781 ttttcaataa aagcactggt gcacgaattg aaatttttct gctgcagccc gaaaaaaaca
     6841 cagattacca gctggcactc tcatcaaaac aatcatgcag ttggaacgta ctgattggca
     6901 atgcagccaa atggaaaagc ggcgatctct ccatcggatt tcagctaaca ggcaacacaa
     6961 cagggatcct gactgcaagc cgtacatcag acaatacagt attgttcagc tggaacgccg
     7021 atattgaatt ttctgaagta ttgtcgcttc tggcgcgcgt cccgcttcca ccctatattc
     7081 gcagagatac ggttatagaa gaccgcgacc gctatcaaac cgtgtttgcg cgaaacgaag
     7141 gatctgttgc cgctccgact gccggactgc atttcacaac agaacaactg aatgaactca
     7201 accaaaatgg catcatgcag attgcactga cactgcatgt aggacttggc acattcagac
     7261 cggttaaagg aaatatcgcc gatcatgaaa tgcacaccga accatttttt gtttcatcag
     7321 aacaactaaa tcaactaacc gaaaatgcag acagaccttg gatagtagta ggcaccacca
     7381 ctctgcgggc acttgaaagt ctgtatattt ttgcagaaaa aataaaatcc ggagaaagca
     7441 ctgcctctgc ggaattcaat attgcacaat gggataaatg gaacagcggc aacaccctgt
     7501 cccgaaacga agctttcagc attttgaaac aattaaatgc aggaaaaaac acaggacttt
     7561 tcggaaacac atccctgttt atcaccaagg gaaaacctgt aaaaacggct gattatctta
     7621 tcaccaattt ccaccagcca caaagcacct tgctcatgct ggtcgatgcc tttgcctcag
     7681 agcaatggca aaaggcctac gaatttgcca ttcaaaatca aatgcgattt ctcagctatg
     7741 gcgatgcctg cctttttaaa aacaggtttc tgtaaatcag tctgttaata ttttatctcc
     7801 atttattcag ttttgattac ttttgttcct ttaattaaaa aagacaaaag atggacacgt
     7861 ccacgctcaa actggaaaat ctgaaaagcc tgatgcctgc ggaattcact cccggacaaa
     7921 ttgccgaagc tcaaacaata tttctaaaaa aactatccga actctctcat cgtcattacg
     7981 gaggaaaaat ccagaccgtt ccgaaagccc ctgtgcccgg cttcaactgg tttaatgtat
     8041 ggtacactcc gggtgtttcg tcggtttcaa ccggcatacg cgacaacaat gatctctcgt
     8101 ttgaactttc aaaccgcggc aatctggttg ccgttatcag cgacagtaca cgggttctgg
     8161 gcgatggcga tgtaactcca tccggtgggc ttggcgtgat ggaaggaaag gcctatctga
     8221 tgaaatatct gggcggagtt gatggggttg cactgtgcgt tgacagcaag ggaaaagacg
     8281 gaaaaaacga tcctgacaaa atcattgaat ttgttaaaat ggtacagcac agttttggag
     8341 ctgtaaacct cgaagacatc agtcaaccga attgttttaa agtattggat gtccttcgtg
     8401 aagaatgcga tataccagta tggcacgacg atgcacaggg cacagccagt gtaacgctgg
     8461 ccggagtaat aaatgcgctg aaactggccg gaaaaaaaat gagtgatgcc cgctttgttc
     8521 ttttcggtgc cggagcatcc aataccacca ttgcacggct gcttatcacc gacggagcca
     8581 atcccgccaa tatcatcatt tttgacagca aaggttcttt aaataaaacc cgaaaggata
     8641 ttgagtccga cacccgtttt taccgcaaat gggaattgtg ttgcagcacc aatccgcaat
     8701 gcacctccac cttggaagaa gcaatgaacg gtgccgatgt gcttatatct ctttcaacgc
     8761 cggggcccga aaccattaaa caagactgga tcagacgaat ggcgccaaaa tccattgttt
     8821 ttgtatgtgc caatccggtg cctgaaatat atccctatgc agccaaagaa gcaggagctt
     8881 ttattgttgc tacaggtcgc ggtgattttc caaaccaggt caacaattcc atcggttttc
     8941 cgggaattct gaaaggagca ttgatggtca gagcccgtaa aatcactgac aacatggcca
     9001 ttgcagcagc ccattcgctg gccggttatg ccgaaaaacg cggcatcgac cccgaaaaca
     9061 ttgtaccaac catggatgaa gccgatgttt ttccattcga ggcagccgat gttgccatgc
     9121 aggccatcaa agacggtgtt gcacgaaaac ctctttcgca cgaagaagct tatcaatggg
     9181 caaaaaaaga cattgaatat tcgcgaaatc tcgtgaaatc aatgtcagat ggcggctttt
     9241 tgcccgatcc acccgccgct gtcattgaac aagctctgga atttgcaatc aataaggtta
     9301 aaaaataata tgattcattt tctcgaaggc aaagttgctg aaataaaccc tgcatacgtt
     9361 gttattaatt gcaacggaat cggatatttt gtgagtattt cgcttaacac ctatgaaaaa
     9421 attcaaggtc agcaaaatgt aaaacttctc acacatcaca tttttcgcga agacggacaa
     9481 tttctttttg gatttcattc agaagaagaa cgggttgttt tcagattgct gctgggtgtt
     9541 tcgggtgttg gagccaatac ggccagggtt attctttcgg ccatgaatcc ttccggagtg
     9601 gttgaagctg tgagtagcaa caacgtggcc ctgcttcaaa aagtgaaagg aatcggacag
     9661 aaaacagcag aaagaatcat tgtcgattta cgcggaaaaa tagacaaagg cgcatatatt
     9721 tcagaaaata tttcgccaca gcacaataca atacgacaag aagcgttatc tgcattggct
     9781 cagcttggct ttgcacgtca ggccgctgaa aaagctgtag atcaggcatt aaagtccggc
     9841 gacctgactt cagtagaaga tgttattcgt ctggcattga aatatttgta gaacaaacaa
     9901 gaaggaatag gtgaaaggtc ttgtatattt tcctgttgtc acaacgattg ctctggttgc
     9961 agtgcagatg tttacgcctg caccacaaaa aagaaaaatc agtttcgaac cccggcaggt
    10021 cattactatg cctgctgact ccgactcact ggcctatcct tttcaggatg atccttttta
    10081 ttcaactcag gacaacaatc acccgctttt tctcaacgat ccgaaaaata taaaagatac
    10141 cgtagaattt gaccctgaga aaaatgaata cattttcaaa cgtcagattg gaagcattga
    10201 ttacaggccc tctttcagaa tggatcagga tgaatatttc aaatacgact tcgacaaagc
    10261 catcagagat tactggcatg atagggcaat ggcctcagga aaaagaaatg gagcaggcgg
    10321 catttccatc aatattccag gcatgggtgg cgaaagcatt tttggttcaa acaccatcga
    10381 catacgtccg tcgggaactg ttgaactcat tttcggagta aatgcaaacc ggcgtgatga
    10441 ccctgctctc aacgttaaac aaagacgtac agccaatttc gactttcagg aaaaaattca
    10501 gatgaatgtt caggccaaaa tcggcgacaa agtcgatttt ggagttaaat acaatacaga
    10561 atcttccttt gaattcgaaa ataaaatgaa acttcagtac gaaggcaagg aagatgaaat
    10621 cattaaaagc atcgaagcag gagacgttac cctgccactc aatacaaccc tcatcaacgg
    10681 aagccagagt ctttttggta tcaaatcgaa actgcagttt ggtaaggtta ccgtaacttc
    10741 ggttatttcg caacaaaaaa gccagacttc aaacattaca gtttcaggtg gagcacaaac
    10801 cagcgaattt gaactctatt gcgacgaata cgaggaaaaa aagcacttcc tgataggcca
    10861 ttatttcaga aacaactaca accaatggct tgaaaacctt cccattgttg gctcaccaat
    10921 caatattaca aaaattgaag tctgggtaac caacatcggt gcagcaactc aggaaaaccg
    10981 taacattgtt gcgctcatgg atcttggcga acaaactcca tacaatcccg tggttccgct
    11041 cagcatgaat ccagatgcag aattccctga caatgagtca aacggtatgt ttgacattat
    11101 cgacgtcaat caggtacgtg acatcaacag tgttaattca tacatgaaca gcaccggact
    11161 tgttgcaggc accgattatg aaaaaattga aaacgcccgg ctactgtcgc ctaccgaata
    11221 cacctacaac tcaaaacttg gcttcatttc actcaattcg accctcaatg atgatcaggt
    11281 actggctgtt gcatttcaat ataccattct tggttatgac agtgtttttc aggtagggga
    11341 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttttcg attttatcga
    11461 caatgccgcc accaatggtg gaaccattaa ttcaaaaaac ggacgaatct ttttcccgtt
    11521 actggaacct tttggagaag acctgcgcga actcttgggc gatgcagcac tggccgataa
    11581 atattgctat gactcgctat ataccatgac caaaaccggg gcgcggcaat tcccaaataa
    11641 aaaccgcttt accattgccg gggcattcaa atcatccgtc ggaaatgaaa tttccctcaa
    11701 tgcaatgaat ataccgcagg gaagtgtaaa agttactgcc ggcggaattc ttcttgccga
    11761 aaatgtcgat tatacggttg actacactct gggtcgcctg aaaatcatta acgaaggaat
    11821 tctcaatagc ggagccccta tcaatatcag tcttgaaagc aattctcttt tttcaattca
    11881 gacaaaaaca tttcttggaa cacatatcga ctacgatgtc aacaaaaatt tcctggttgg
    11941 cgccaccatt atgaatctga ccgaaaggcc ctatacttat aaagtgaaca taggcgaaga
    12001 acctatttca aacaccatgt gggggctcaa cctgtcgtat cagaccgaat cgcgtctgat
    12061 taccaaactg gttgattttt taccaatgat tgaaacaaaa gccccgtcga gagtaaccat
    12121 cgatgccgaa gtagctaacc tgattccagg tcatgccaga gccatcggca aaacaggcac
    12181 cgcctacatc gacgactttg aaggtagtaa atcatcgatt gatattaaaa acattggcac
    12241 ctggtttcat gcttccattc ctcagggaca gccgtctttc tttcctgaag cttccaatga
    12301 cagcacgctc accagtggtt tcaaccgcgc ccgccttgca tggtatgtga ttgatccgct
    12361 tttcttccgc gacaacaacc tcactcctga ccatattgcc aacgatccgg ccatgctgtc
    12421 gaatcattac atgcgcgaag tgctcgagac cgaggttttt ccaaagaagg aaaacgtcaa
    12481 caatattgtc accaacattg cggtgcttga catggcctac tatcctactg aaaaaggacc
    12541 atacaacttc gatgtgaatc cgactgctgt gtctgccggc atcgatgccg acggttatct
    12601 gctcgaccca tctactcgct gggccggaat gatgcgcaaa atcgaaacac ccgactttga
    12661 agaaaccaat gttgaataca tcgaattctg gatgatggat ccgttcatct acaatcctgc
    12721 acatacaggc ggtaaactga ttttcaacct tggagacatt tcggaagacg tgctgcgcga
    12781 ccaccgcaag agttacgaaa acggacttcc aacatccagt gtaatcgaaa atgttgacac
    12841 cactcaatgg ggacgtgttc cgattttgca ggccctggtc aattcttttg acaatgatcc
    12901 cacctcaaga caatatcagg atattggact cgacgggctt tccgatgcag acgaagcatc
    12961 gttctacgcc actgcttatc tcgatgcaat tgcctctgtc ttcggtacaa cttcacaggc
    13021 atacagcaaa gccgccgatg atccttcgaa cgacaatttc cattatttcc gcggatcgga
    13081 ttatgatgcc gatgaactca atatccttga aagatataaa atgtacaacg gtgttgacgg
    13141 caacagtccc accagcgacc aatcgcccga agactacccg acctcatcat caacactacc
    13201 caacgttgaa gacatcaacc gcgacaacac cctcaacgaa tccgaaagat attttcaata
    13261 tgtgattgac atgcagccat ccaaaatggt ggttggtcaa aattacatta ccgatgtttt
    13321 caatgcttcg gccaaccttg ccgacggaac aacagccaat gtgaaatggt atcagttcaa
    13381 aattccggta aaagacccgg atcaggttat cggagatatt tatgatttta aatccattcg
    13441 ctttatccgc attctgatgc gtgaattttc ggaaccgatt gttttacgat ttgccacact
    13501 ggaactggca cgcagccaat ggagaaaata cgaaaatgca ctgctctatc cgggcgaata
    13561 tattccgggc gatgagcaaa acggaacttc atttgacatt tcagctgtaa atattgaaga
    13621 aaacggcagc cgcgaaccca tcccttatgt tttaccacca agtattgaac gcgaagttgg
    13681 ctggggatca aataccatgt atgaaatgaa cgagcaggcg cttgttctca aaacatgtaa
    13741 cctcattgat ggcgatgcac gtgcagtgta taaaacatgc gaattcgaca ttcgacagta
    13801 caaaacactg aaaatgttca tccactccga gctttcacaa cccggtgcat ataccctcaa
    13861 tgatggcgac ctctctgttt ttatacgttt gggaaccgac tttacggata acttctatga
    13921 gtatgaaata ccgctgcacc caaccgatct caatgtcagt tcacaaaacg cagacatcaa
    13981 tcatctggtt tgggccgact cacttgatct gaatcttgac agtctcattg aattgaaaaa
    14041 agcacgcaat atagctatgc gacaaaacaa ttcaggcgta actcttctga caccatacta
    14101 taaatacgac ggactgaaca aaatgaccat tgttggaatt cccaatctaa gcgatgtcag
    14161 aaccatcatg attggtatcc gcaatccaaa acgcaaccct gtcaatctct ctgatgacgg
    14221 tcaggccaaa tgcgccgaag tctgggtcaa cgaattgcgt ctgaccgatt ttgacgaaga
    14281 tggcggatgg gctgcaacca cccgcatcac tgccgatttg gccgatattg gtaatatagc
    14341 cgtagtaggc aatatgtcaa ccgccggctt cggaagcatt gagaaaaaag tttctgaacg
    14401 ccagaaagaa aatattatgt cttatgatct ggctaccaac attgaacttg gaaaattttt
    14461 acccgaaaaa acaggactca aaatccctat gcatttcgat gtgagcgaaa cattcagcaa
    14521 cccgcaatat aatccattaa accccgacgt actgctcacc gaagacctga acacctacag
    14581 cgatccggtc gaaagagact ccatcatgca attggtacaa agctataccc ggcgcaagag
    14641 tcttaacttt gtcaatgtaa gaaaagaaaa aaccggcacc agtaccaaaa acaggattta
    14701 tgacctgtca aattttgacc tgacttatgc atacaacgaa gtattcatac gaaatattga
    14761 tgttgaatac aacctgacaa agacataccg tggagctctt ggctataatt acacatccac
    14821 acccaaaaat tatattcctt tcaaaaaagt gaaatttctc aataaaaaag cgctcagatt
    14881 tttcggagac ttcaatttct atctgctgcc caagtcttta tcgttcagaa cagatctgga
    14941 tcggggctac gaagagaatc tgctccgaaa taaatcagaa gcactcattt taattgaacc
    15001 aacttacctg aaaacttttt cttggaaccg gacttattcg ctcaaatacg accttaccaa
    15061 aagcattaaa atagactata ttgccaacgc caatgcccgc attgacgagc cacccggaag
    15121 cattgataaa gccgacagcg actatgaagc aaaaatggat tcaatctggc agaatatcaa
    15181 atccctgggg cgtgttacca attacaatca atcgctcaaa ataaattatg cattgcccat
    15241 caacaaactt ccaatgctcg actggatcag ttcttcggtg caatatggcg gcgatttcaa
    15301 gtggacagct gcacccctct acaaagatac attgggcaaa tatgccgaaa gcccatatgg
    15361 aaataccatc gaaaacaacc gttccatttc ggccaatatc aatggtaatc tggttaacct
    15421 ctacaacaaa atcccttatc tcaaaaaact caaccaggcc tcgaccaaac caaagcaaaa
    15481 tgtaaactcc cgtgccgaag ccatgttgaa tgacactacg ggcaacgcaa aaaaagacac
    15541 catacaactg tttaagaaaa ttgccgataa tgttcttaaa ctgatgatga tgaccaaaaa
    15601 cgcttccctt tcctacacat tggccgaagg aacctacctg cctggctatc gcggaggcat
    15661 ggacattctg ggaatggacc ttgacaatca ggcacccggt tggggatttg tttttggcga
    15721 tcagcgcgac atcagaccat cgctggttca acacaatctg ctctctaagg actccctttt
    15781 caacaatgcc tatgctaaac gcaaaaatga aaatctgaat gcgcgcgttt cgattgaacc
    15841 agtgaagaat atgaaaattg aaattaccgc tatgcggaat ttcaccgaaa accatagtga
    15901 atatttcaaa ttcgacgaag ccagcaacgc tttcagatct tactccggaa ttgacaatgg
    15961 cagtttcagc ttatcaattg ttacctggag caccgcattt attcaggact acgacgatta
    16021 ttcaaacaaa acattcgaat atttcaaaac cataagaccc gaaattgccg ctcaggttgc
    16081 ccagaacagt cctatttggg atggaaccta cgttaccgac tccactaccg ggcagattta
    16141 tcccaacgga tatggcccca cttcgcagga tgtgcttatt ccagcttttc ttgcggccta
    16201 caccggcaac aatcccgaaa aatacataag ttcaaaattt ccacgaattc cgctccccaa
    16261 ctggcgtttg acctacaacg gactgacaca gatcccctgg gtgaaaaaaa gatttaaaag
    16321 tgccaccatt tctcacgctt accgctgcac atacaacatt ggcggatact cttccaatgt
    16381 gcttttctcc gattccgacg ctgacggcta cactactgta aaagatgcgc tgggcaatta
    16441 tctgccaaaa tttgaaattg cacaggtaag catcaccgaa cagttcagcc cgcttatgaa
    16501 cattgatttg acctggaaaa acagtcttat cagcaaaatt gaatacaaaa aatcacgcaa
    16561 tctcgctgta agttttgcca ataaccaaat gaccgaaatc atttcaggtg aaataacgct
    16621 gggttttggg tacagattta aagacgtaga atttgaaatc tccggaaaag attttaaaag
    16681 cgacctgagt ctgaaagccg atttttcgat ccgaaacaac aaaaccgttc ttcgcaaaat
    16741 tgttgaagat gttgaccaga taagtgccgg acaaaaagtt atttccatca acctctcggc
    16801 agagtacatg ctaagccaga aatttaccat aagggccttt ttcgacaaag taatcaacaa
    16861 cccctacatt tcatcacaat tcctgaacag caacaccaac gctggtatca gtctccggtt
    16921 ttcactggct caataatttt tcaatctggc attgccggat ttaaaaaaat agtgtaattt
    16981 tgggcattaa aaacaaattg gcaatgaata ttcctgaaaa cttactctac accaaaagtc
    17041 atgaatggat tcgcgttgaa agcgaaaccg gttttatcgg cgtcagtgat tttgcacagt
    17101 cagaactcgg cgatgtcgtt tttgtagaag ttgaaaccgt tggcgaaact ctcgaccgcg
    17161 aagaagcgct tggaaccatc gaagcagtga aaactgtaac cgacgtttac atgcctgttg
    17221 gcggcgaagt gctcgaattc aacgaaaaag taaaagaaac acccgacctg atcaataaag
    17281 atccttacgg cgaaggatgg attgttaaaa ttaaaatttc aaacccaacc gagcttaacg
    17341 atcttatgaa tgccgaaagc tacaaaggac acacgggcca ctaattttat cacagaatat
    17401 cagctaattc ctgttttcag gccataaact tatctttacc ggcctttttc tgaaataata
    17461 tttgtggaat tgctgcttta tttcatcttt gcttagaatc ttaaaattat ttaccatgga
    17521 tatcaagaaa aacccgaaat acgaccttga aagatacaag ggtcttttcc tcgaagctgg
    17581 gctggttata gcactgggtt taatgctgat ggctttcgag tggagaaaat acgacaacag
    17641 caatgatggt tttgaacaaa tgcaggttgt cgaaattcct gaagaaatgg ttgaaatcac
    17701 ccgtcaggaa gaaccagaac cacccaaacc ggaaccacag acaacccttc tcgaaattgt
    17761 tgatgacgac attgaagttc aggacgaact cgatatcaat gccgaggatg atcaggatac
    17821 tgaagtgggc gattttgtcg ctccggttga tgatggcgag gaagttgaaa ttgttgaagc
    17881 agaaattttt accattgttg aagaacaacc cggttttccc ggtggcgaat ctgccttaat
    17941 ggactatctc cgaaacaaca ttaagtatcc gcaactggca cgtgaaagta atattcaggg
    18001 aacagtattc gtgacttttg ttgtcgagcc caacggcgcc attacaaacg tcaaggttct
    18061 tcgtggtatt ggtggtggtt gcgatgatga ggccacacgc gttgtaaaaa acatgcccgc
    18121 ctggacacct gggaaacagc gtggaaaacc tgttcgcgtt caattcaacc tgcctatcag
    18181 atttattctt gcaggttaat caataatgat ctcttttaaa aaccggcctt tgccggtttt
    18241 ttttatgcct tttacttttc agaatagcat ctacactgag caggcattgc aaaacacgct
    18301 atgcagccat aacttcaatc aattaatctt taaaatcaaa gttatggaca caaaaaagaa
    18361 caaatcgaaa aaccttgaaa acttcaaaag tatgtttttc gaaaccggtt tggttgtttc
    18421 actgtttctg gtgcttgcag ccttcgaatg gaaaacctct ccttcggtaa attcaacact
    18481 gaccgaaaca ccctatgtgt atattgatac cgacacggtt attaaattca aaaccataga
    18541 aaaaactcct ccgcccaaag taatcaccga aataaaagtt acaaaaaaag aagttgaaac
    18601 aacaccggaa attttcaaca ccgaatggga tgataaagcg tcggaaaaag ttttcactga
    18661 aatcaaaaaa gatgaaaccg aacctcaggt agaaggcgat gaaattatta ctgttccgcc
    18721 gcttatgccc cagtttccgg gtggtgatga ggccctgatg gattatatga tgaaaaacac
    18781 caaatatccg atgcaggatc gtgaaattgg tttgcaaggc aaagtctatg tttcctttgt
    18841 tgttgaaaaa agcggcgaag tcagtcaggt aaaattggaa cgcggcattg gaggacaatg
    18901 cgacggagaa gccatgagag tagtaaaaaa catgccttcg tgggtacccg gaaaaaacag
    18961 tctgggcaga ccggtcaggg ttaaactgac tttgccggta agctttattc tgataaacta
    19021 agactcaacc tgaacaatac ggaaaacctt gtcgccacga cgaaccatcc gggatacaga
    19081 aaaggcaaac aacgcgccct gaggcgccat gggcgcttct ccacggtcaa gatgaatttt
    19141 atcgatttct ccctcataca ggcccgttgt cgggcctgtt atggtaactg ttgttcccgg
    19201 aaataccggc cctgtttcgg cacgcacttc ggcaacacca agttttgaaa aataattagt
    19261 gactttgccg atatattcac gacgttcggt agccgaagac ccatgccggt cggtccattc
    19321 gccaatggtt tttcccatat agtacccttc ccagaaacca cggttataaa ctttggaaag
    19381 tcgttccatc catgatgcaa tagccgcatc ggtataagta ttgttttcaa tggctttcaa
    19441 tgcttcggaa tacactctta caacagccga tacatattcc ggcggacggc cgcgcccctc
    19501 aattttgagc accgaaatac cggctgcgat aagcttatca agaaagccaa gcgtacacaa
    19561 atcgcgtggc gacatgatgt actcattatc aatcaacaat tctgttcctc cctcaagatc
    19621 cctgaccaga taaggtctgc gacatatctg cgcacattgg ccccggttgg cgctccggtt
    19681 cagattatcg agactcagat aacatttgcc actgatggcc atacacaaag ccccatgggc
    19741 aaaagcttcg agccgcacca gattgcccga agggccacag atattttgct gaataatctt
    19801 tgcatgcaac tgcttcactt tttcaagcga caactccctt gataaaacca tcacatcggc
    19861 ccagcgcgaa aaaaaacgca ctgcaccagc attggttatg tttgactgtg ttgacatatg
    19921 cacagttaat cctaactctc tggaacgctc aagaacgctg acatcggtgg caataacagc
    19981 atcaattcct gcatttgcag cactctgtaa aatactattg acttcgtcgg cttcattgtc
    20041 aaaaacaaca gtattcaaag caagataagc cctgacgcca ctttcgcggc ataatcgcac
    20101 aatatttgca agatcttcct gcccaaaatt tacagccgat gaagatctca tgttcagatt
    20161 tcctattcca aaatacacag aatcagctcc ggcctcaagc gcagcgcgca gcgattcata
    20221 agatccaacc ggtgcagtaa tttcaatttt catagaacaa agatagagaa agaatagttt
    20281 aaattttttg cgaaataatc atcaaaccga tatattttaa tatctttgaa acaacaaacc
    20341 tttgaaacaa ctaaaaccta ccctatgaaa aagatttttt tactgctcgg aattgcgttt
    20401 gctgctacaa tgatttcatg cggtccaacc accgaagatg ccatcaaaca caatgacaac
    20461 attgttgctg accagaaaga aatgcttgat cttgaaaccg atttgttaaa cgacatcgtt
    20521 gacggacaag aagcggcaga catcaaaaaa accttcaaga aatatctcgg ttttctcgaa
    20581 gaaatggaaa agaaatataa cgaaatggat gagtttgatg aaaaagacac attcagaaaa
    20641 gccatgatca atcttatcaa ggaattcaaa agtgttgcag aaaatgaata cgatgaaatg
    20701 attgagattt actcaaaaga accagatgct ttaaccgatg cggattttga gaaatgggaa
    20761 gacctcaccg aaatcattga tgaaaaagaa ggcaaagcca atgatgattt tctggatgct
    20821 caaaaagaat tctccaaaga atacaatttt gatcttaaaa aataattgaa aaacattcta
    20881 tttgaaaggg ctgtcatcat atggcggcct tttcttttta ctttctgaaa aaaacagaca
    20941 tctgcccgct gtattttatt ctataccttt gttgcgcaaa tgcaatacct tttttcaatg
    21001 aaaaaactga ttgtcatcac agctttcatc attgctgctt tttcatcctg cggtccttct
    21061 gaacaaaaac gacaggatgc caatgatgta tattctgaaa cactcggcat tataaacgaa
    21121 gtcaacacac atgacagcga atatgtttac tgtatgcaat atttgttgcg tgaaatacag
    21181 tcaccagacc tgaaaaaaaa taaaaagaaa ctgaaatcaa ttacagatag tatttcatta
    21241 ctcgatgcag cctgcgattc actagctatt gtcattgcag aggcaaaaac aaagaccgaa
    21301 aaactgaatg tcgaaaaacc agatatggaa ttgactgaag cagccgacag tctgctaagc
    21361 aaatacaata caattacaca aaatatttat caggatatca accaaaaaat gaaaaagatt
    21421 tcgctaccgg taaaagacag cgaatacacc gctttgcttc gcctgtcata cagagccgac
    21481 tccgtgctta atgctgcagt tacaaatttc aatactgaaa gtgcagcatt ttacgaaaaa
    21541 tacggactaa aagatttaaa aaaataattt ttcggagttt tctgagaaga aaaattattc
    21601 tggctttacc agacggatac tgagttcgtc aagttgtttc tgatcaacaa acgaaggcga
    21661 atcagccatc aaatcgcgac ctgagttgtt tttcggaaat gcaataaaat cgcggatatt
    21721 gttctgcccg gccatcattg cagcaaatct gtcgaatccg aaagcgattc caccgtgcgg
    21781 aggcgcacca aattcgaaag ctccaagaag aaaaccaaac tgatcagcag cttcttcttc
    21841 ggtaaaacca agagctctga acatgcgcat ctgcaagccg cggtcatgaa ttctgatact
    21901 gcctccgcca atttcgacgc cattaataac aagatcatat gcattggcac gaaccgaacc
    21961 cgggtcagat tcaagcattg atgcatcttc ggccaccggc gaagtaaacg gatggtgcat
    22021 ggcaaaaaag cgttgagcat catcatccca ctccagcaac ggaaaatcaa caacccaaag
    22081 tgcagcgtac gttgatgaat cgcgaaatcc aagacggttt ccaagctcaa gacggagatc
    22141 gccggcaatc ccaaaaattt tcttcttatt accggcaaca atcagcatta aatcgccttc
    22201 accggcagca caagaacttt tccaggcatt caggtcatca gccgtaaaaa atttatcaac
    22261 cgatgatttt gcagttccat cgcttgaaaa ttttgcccaa atcagacccg tagcaccaat
    22321 ctgcggcctt ttcacccact cggtaagttc atccagctgc ttacgtgtat attctgcggc
    22381 acccggcacc acaagcgcca gcacatgatc ggccgaatca aacacctgaa atccttttcc
    22441 cttcgcaatt tcagaaatat cctgaatttg cattccaaaa cgaagatccg gtttatcact
    22501 gccataaagc cgtatagctt cactataagg catacggata aattcaggga gctccgtatt
    22561 tttaattttt ctaaacaact gtctggtcag accttcaaac acattgagca catcttcacg
    22621 ttcaacaaaa gacatttcac agtcaatctg tgtgaattcc ggctggcggt cggcccgcaa
    22681 atcttcgtca cgaaaacaac ggacaatctg aaaataacga tcgaaacccg acaccatgag
    22741 caattgctta aaagtttgcg gggactgcgg cagagcataa aactgatttg tattcatgcg
    22801 cgaagggaca atgaaatcgc gcgctccttc aggtgttgag cgaatcaaat acggcgtttc
    22861 aatttccatg aaattatttt cactcagata atttctaacc tcgagagcca tacgatgacg
    22921 cagctgtata ttaaactgca gcggagcccg gcgtaaatcg agatagcggt atttcatacg
    22981 aagttcttca ccaccatcag tatctgtttc aatggtaaaa ggcggaactt gtgatttatt
    23041 gagcacttca agcttctgaa cctcaatctc aatatcaccg gtattaatat tcttgttttt
    23101 attggagcgt tccctgacaa tgcctgtcac cctgattaca tattcgcgtc ccagactgcg
    23161 tgccgtttca cacaaatcct tgttggcatc aaggtcaaat atcaactgag taataccata
    23221 acgatcgcgc agatcaataa aagtcattcc gccgaggtca cggattcgtt gaacccagcc
    23281 actgagtaca actgtctgtt caatattttc ctgacgcaat tcgccgcaag tatgagttct
    23341 gtacatagag aaaaattttt tgcgaaatta caataatcaa tcgttttaat agtttaattt
    23401 tacatccctg agataactat ggctgacaga attctactat tttttctttt tttattatcg
    23461 attgttgtag ccaacagtca ggaaaacctt atttataacg gagattttga gacctaccga
    23521 aactgtccgg aagattatac ccactatctc gaaggaatta cagaactagc tcccggctgg
    23581 tttatcagca acacatcgac tcctgattat tttaaccgat gcagcataaa gagcaaggtt
    23641 ggcatcccgg ccaattttgc aggcaacatg gaaccccatt cgggcaatgc ctatgttgga
    23701 atgattctgc gcgccgattc atcaagatat ccatactccc gggggtacaa cgagcatttg
    23761 caaaccgtta ttaccatgcc tttggaacga aacaaattat attgtctcga attttattat
    23821 gtcttatcaa ataatagtgg catagcttcc aatggcttat cggtatatct gagcaacgca
    23881 aaacctgtat ttgacgaagc cgccgaaaaa tttccctttg tgcctcaact tgaaatcaga
    23941 cgcgatagcg tgctgaatga gcgcaaccaa tggaatctgt tttcaggtgt tttcagagcc
    24001 agtggagaag agaaatacct ggtgatcgga aactttaccg attataccga aacaaaatat
    24061 gttcctattg ttgaacaagc cactacgcag attcattcat ttgcctatta tcttttcgac
    24121 gatttcagat tataccggat tgataatgaa gatgaatgct tatgcaatgt gatacaaaac
    24181 cagaccgatt catttccgcc catacaattt gcaaaagaag attctgtttt catggaagat
    24241 attgaaatag gcgaaaccat tattctccgc aacgtttatt tcgaattcga caaaaccgaa
    24301 ttgctcccct actcttttgc agaactcaat aaaatttaca atatcctgat tcagtatcct
    24361 gcaatgacga ttgaaatctc aggacacacc gacaatatag gcacagaacg ctataatcag
    24421 gcgttgagcg aggaaagggc aaaagctgtt ctggaatacc tgtacaacaa aggcatagag
    24481 cttaagcgaa tgaggtatgc tggaaacgga agccggcttc ccattgccga caacaattta
    24541 cctgaagggc gacagatgaa ccgacgcgtt gaaataaaaa tactaagcaa ataatggaga
    24601 atcaggatga aaaatttatg caggaagctt tgaaggaagc atttaaagca tttgcaaaca
    24661 acgaagtacc tattggtgcc gtgctggtgc acaacaaccg cattgtggcg cgcgcataca
    24721 atcagaccga actgctcaaa gatgtaaccg cacatgctga aatactactg atcaccgcat
    24781 atagtgccga ttcgggaatg aaatacctgg aagatttcac cctgtatgtt accctcgaac
    24841 cctgtgccat gtgtgcggga gcgatgaaat gggcccgaat cggccggctt gtttatgctg
    24901 ctgacgattt aaaattcgga tatacaacac tttccgagaa cattcttcat ccaaaaacca
    24961 atattaccaa aggcgtcttt caaaaagaat cggctcaact gatgaaagac ttcttcgcca
    25021 aaaaacgtct ttaatgacaa atttgttagc ttttagaaac aaatacgtct atttgttctt
    25081 gaatatctga gattaattat tacttttgca cattaaataa aaaataatta actcatgagc
    25141 agaaatatct ttctgacctt tctgttggtt tttacagctg tatcgttgat gtcgcaaccc
    25201 atcgatgata ttgcaaaaat gcagaatgac aagaaataca gagatgcata tgataaggca
    25261 gaagctgcat tgctggattt taattacgaa gaagccctgg aaatttatct gaaatgggat
    25321 tccgtttacc ccaacaatgc caatctcaat ttcaaaatcg gctattgcta tgccaatatg
    25381 agcagcgata aaccccgcgc cattccttat cttgagaaag cggcatcctc tgtggtacgt
    25441 gaatatgtgg gcgattatat tgagagcaat gctcctgtcg atttattcat gcttcttgga
    25501 attgtttatc acatcgatta taaattcaac aaagctgttg aaaattttga caaattcatc
    25561 aaatacgctg agttcgacga tcccgagctg gttgattcgg ttaaagtcct caaggaaaaa
    25621 tcatacacag ccatccgtgt catcaacaac ccactgagca ttaaaatcga aaacctgggg
    25681 gaacttgtaa acacaaatta tccggaatac agtccggtca ttacatcgga tatgaaaacg
    25741 ctgtatttta ccagccgaag ggaaggaagc accggagaca aaaaagatgc aaaaggactt
    25801 tttttcgaag acgtatatta ttcttcgctg ggctctgata accgctggtc aacggcaaaa
    25861 cacatcggag ggaaaatcaa taccgttggt cacgaagcaa ccatcagcat ttcgcccgat
    25921 ggcaaaacca tccttctgta tcgcgacgac aagggcgatg gcaacattta tttctccgaa
    25981 ttgcttgaaa acaatgaatg gagcaaaccg gaaaaacttc cggaacccat caattccaaa
    26041 tcatgggaaa cacatgcctg tttttcaccg gacatgaatt tcatttattt cgtttccaac
    26101 cgccccggtg gttatggtgg tcgtgacatc tgggtttccg aaaaaatcgg agggcactgg
    26161 acagaggcgg aaaatctcgg acccacaatc aatactcctt atgacgaaga ctccccctat
    26221 atgcttcctg atggaatgac attgtatttt tcttcacagg gacatgaaaa catgggtgga
    26281 ttcgatattt tcatcagcac catttcggaa gatggtttct ggtcaactcc agaaaacatg
    26341 ggctatccgg taaacactac cgaagatgat gtattttaca ttccaacacc agatgaaaaa
    26401 cacgcttatt actcatcctc caaaaattac ggaattggtg gtcaggattt gtattatatg
    26461 accattgtca aagccaagaa aaaaaccatt gcattgcgag gtcgcgttgc tgatgccaat
    26521 tcatacaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct caaccgtctt
    26641 gttaaactta tgactgacct gcctactctt aaaatcgaaa tcatgagtca caccgacaat
    26701 atcggatccg atgcatataa tctggatctt tccaatcgtc gcgccgaatc agtggtatcg
    26761 tttttgataa agtccggcat ttcaaaagac cgattggtag ccaaaggtta tggtgaatca
    26821 cagcctgtag ctacaaacac caccgatgaa ggacgtcagg ccaatcgccg tactgaattc
    26881 cggattctta gcaaataact gatttgctga aaaaatgtat ttttgtcaaa cgaaagaaaa
    26941 taaagctgtt atgaaaaagt tgttttttgt tttcctggcc gccattttat ccatctcaaa
    27001 ggtctctgcc gatgaaggaa tgtggcttcc gctgctggtg gaccggttga actacaccga
    27061 tatgcagaaa atggggcttc agctgactgc tgaagagatt tacagcataa accacgcaag
    27121 cctcaaggat gccattgttt cgatgaacga tggcatgtgc accgcattca tggtttcgaa
    27181 ggaaggtctc atgatgacca atcaccattg cggtctggag tatatcactg aaaattccat
    27241 caagtacaat tctgattatg ttcagaatgg cttctgggca ttggataaaa aacaggagtt
    27301 gtacaacccc aacctgaaag ccactttcct ggtgcgcgtc gaagatgtaa gccagaaagt
    27361 gctgtctcag ctcaaggaaa caatgactcc ggctgaacgt gaagccaaag taaatgaaat
    27421 ttcagccaaa ctggtaagcg atgcagtgaa tggatcacac tacgaaggcg aagtgaaatc
    27481 cttttacaaa ggcaacgagt tttatctctt tatctatgag gttttcagag atgtacgcct
    27541 ggttggtgca ccaccaatgg cacttgggaa ttttggggcc gacgccgaca actggctctg
    27601 gccacgtcat acagcagact ttgctttttt ccgcatatac atgggtcccg atggaaaacc
    27661 atcaccgtat gacaaaagaa aaaacattcc ctatgtgccc aaatattata ttcctgtttc
    27721 tacacagggt gtgaacgagg gtgatttcac catggtactc ggatacccgg gaacaacaga
    27781 ccgcaccatg acttcgagcg gattgaacct aacactcgaa accatcaatc cgtccatcat
    27841 caaaatccgt gattcaaaat tgcgccttat gaaggaagat atggaccgtg acccatctgt
    27901 tcagcttatg tatcgttcca agtacaaccg ttcttcgaat tactggaaat attatattgg
    27961 tcaaattgaa gtcttgaaac gactcaaagc cattgaccag aaaaaacaaa tggaagcaga
    28021 cttcaccaaa tgggtgaatg ccgacgcttc acgcaaatca atatatggta atgccctggc
    28081 cgcaattgat ctggcaacga ccgaaattgg caaataccgc aaagcagtta tctattacag
    28141 ggaatgtgtt ttcagaggtc ctgaaatcat ttcttttgcc aataaattcg actcgctcta
    28201 ttactatctc aacaaaaccg atattcccaa agaacagaaa gccgccaagg tggccgactt
    28261 aagcaccaaa ttgaaatatt atgcatccaa ttattatttc aatggttaca atattgatgt
    28321 tgacaaaaaa atattcattg ccttgcttcg atttatgaaa gatgatgttg gcgctgaatt
    28381 ttatccgact gtttttgagg aagtagataa aaaattcaag gacaattttt caatgtacgc
    28441 gaattttgta tttgaaaaat caattttttc aagcaaagaa atggtgtatg aatttctcag
    28501 agaacccaat gccaaagtcc ttgaaaaaga cccgggtttt atagccatgc tttcattcaa
    28561 cgaaaaatac gatcagataa aagaacagat tgccgtaatt gagcaaaaac ttgaaaacag
    28621 cgaacgttta tacatgcatg gactgatgaa aatgctcgaa ggaaagaaaa aattctatcc
    28681 cgatgccaat agcaccatgc gcatcactta tggaaaagtg accaactata ctccaaccga
    28741 tggtgttgaa aaacctttct acactacact ttcggaactt attgcccgcg aggatgccaa
    28801 aaatccggac tttatcgttc ctgaaaaact gaaagacctt tacaacagaa aagaatatac
    28861 gaattacgcc aatgctgcgg gtgatgtacc tgtttgtttt ctcaccgact gtgatgtaac
    28921 cggcggaaat tcgggtgcac ccattctcaa tgccaaaggc gaactggttg gtattgtatt
    28981 cgatattaac tgggaggcaa cagcatcaag tttaagctat gtgccggatc agacccgaac
    29041 catttctaca gatatccgtt acattctttt cctgattgaa aaatatgccg aggccacaaa
    29101 catcattcag gaacttgaca tcagaaaata gaaaatatta attccgaata gctgcacttc
    29161 gttgcagcta tttttttaca atggcattaa aatccgcatt ggtaaatgct ttcacccaat
    29221 taccaaatgc ccctgattct tcgtttatgc gaagccgaag cgctgaacat atttcatttt
    29281 ttaaatccgg atagcgcctg catatacttt caatcaccaa caatgcattg tgacgaacgg
    29341 ccacgggttg cgccttatcg caggcaaaag caaaacaaac atcgtataac aacccggtat
    29401 cattttcatc catttcgtca acatatcgcg aaagcatgcg caaaaactga cggcgaggac
    29461 catcattttt aaaaaccgta agatgccgga tgattttcgg aatcaactgc cgggcaaagc
    29521 caggtcttaa atcatctgct ttttcaataa catttgaagc acgcatatca aaaggatatt
    29581 catccgaaaa ggccattgtt tcaaaaatct gcaacaactc aggttttttc aacaatacat
    29641 tggcagaata atctgctgtt gccctgcaac tatcaaaaag catttttttt ataacttcaa
    29701 tctgctcatt catttcacaa aggatcaaca tctgtaataa cacgcacacc cttattatcg
    29761 ggatgcgcca gcagttcggc aatctgggct gaaatccatt tcttgtatga acctaattcc
    29821 ttattgcgcg gaagtttgat gagaatattt ttaatgtaaa aatttttgat tctggctacc
    29881 ggcggaaact cagggcccaa cagttgtttt tcattaaact gttttttcaa aagcgctttc
    29941 atcatcgccg aggccttatc agcaactggc ttatccttat gcatcacaga aagacgaatg
    30001 agacgtacaa acggcggata cagataattc atgcgcgatt gcgtttcccg ttccataaaa
    30061 ccaacataat ttccttcggc aacaaaattc acaatcggat tatccttgtc gtaaacctgt
    30121 atgaaaacct tgccgccggc atcacgccgt cccgctctgc ctgccacctg catcatggtc
    30181 tgaaaagcat gctcatgtga tctgaaatca ggaaaggcat gcaaattatc agcattcata
    30241 atgccaacca catcaacgcg actgaaatcg agaccttttg taatcatctg cgtaccaacc
    30301 attatattaa accgcccctc atcaaacccc ttaaacatct ccgacatggc ctttttcccc
    30361 cgggtggtat ccatgtccaa tcgggcaatc ttataatcgg gaaacaaaat ctgcaattca
    30421 tcttcgatgc gctgtgtacc cattcctttc atgacaatct gcgagctttt gcatgcagga
    30481 catttggatg gaaccctgtc ggtatagcca caataatggc atttaagtaa ttccacattt
    30541 ttatgatatg tcatcgagac atcgcaattg cgacaaaccg gtgtccatcc acattcctta
    30601 cattcaatcc aggtgctgta tccacgccgg ttttgaaaaa gaataacctg ccgcttatgc
    30661 atgacggcct tcttgacagc ttcgtacaat tctggactga gtggcccaag catttttttg
    30721 cggaaagttg cctgccgaat atcaataaca tgaacttcag gataaacagc aacaccaaaa
    30781 cgctccgaaa gctcaacacg cgcataaact ccattggcag cattcaccca tgtttcgagt
    30841 gaaggcgtag cagaccccag aataatgccg gcaccagtca tatgtgaaag cataacggcg
    30901 caatcacgtg catgatagcg aggtgcggga tcctgttgct tataactgga atcatgttct
    30961 tcatcaacaa taataagtcc gagattgctg aatggcatga acaaagcaga acgcgcaccc
    31021 agaatgagtt tcggctcact tttttcaagc acttcgttcc atatctctac tttttcctgc
    31081 gcattgaagc gcgaatgata aaccaaaaga tctttgccaa aataagcacg caagcggtcc
    31141 accataaaat cagtcagtgc aatttcgggc aacaaataca atacttgttt tcccgctgcc
    31201 agcatatcgc tgatgaagcg aatataaatt tcagttttcc cactgcctgt aacaccaaac
    31261 agcaacactg gtttattctg atccaatgca accttaacgt cggagtatgc attatcctgc
    31321 gcttcggaaa gctgaaacac aggcgcaggg ccattatcag atttttcatc aagacgcgat
    31381 acgatgcgtt cggactgaac aagaatttct tttttgatta aagccgtaac agcagcagcg
    31441 cctgccactt tattcaattc tgtgaacgaa actacgtttt gtccacgagc cagattgaaa
    31501 tacgtgagca atgcccgaaa ctgattttct gatttttttt caagcttcac catcaaagca
    31561 ttcagtacgc cttcgttttc atattcagca gaaatggcaa caaatttttg cttctttggc
    31621 gtataacgtt cctcgagttc ttcgaccgga tgcagcactt ttttatcgat gagcgacttg
    31681 attaaatgat gcacagcctg acgaccagta atggcttgaa tatcaacgga tgtgagcttt
    31741 tcggctttca gagcggcttc cgcaatcatg tattcatcat cggacaactg actgaaatca
    31801 tcattaaaat ccggatgaat gacataaatg gtattgcttt ctatcttaag tccggctgga
    31861 atagcagcat tcatcacatc gccctgcgga cacatataat acgaagacat ccactcccaa
    31921 aactgcagct ggagaggatt tacaacaggg aacacatcaa gtacatcgga aatttcctta
    31981 atattcattt cctctcccgg cttctgatcg gtaatgcggg cgacaatacc tgtaaaaaat
    32041 ttctttgcgc caaaattcac cacaacacgc ataccaatct ctacctgaac tttcagatga
    32101 tctggtatct gataagtaaa ataaccttta aggggtaatg gcaaaataat ttcggcaaac
    32161 acgtaatgaa attaaacaga taatatatga atcatttctt tcagatcggt acgcacttta
    32221 ttggtatgct ttacagcttc accaaacggt gtttcgacta catcgcgttt tatgatacca
    32281 accataacac ccgatttgcc tgccaggagt gcttttacag catgatagcc atactggctg
    32341 gcatataccc gatccatgca cgaaggcgaa cctccgcgct gaatatgacc caaaacggtt
    32401 acacggacat catatttggg taatcgtttt ttcacctgct ctgcaaccac aaaggcgcca
    32461 ccgctttcat caccttcagc aatcatgatg attccactgg ttttatttct acgacgccca
    32521 ttttcgagtt tatcaatcaa ttcatcaata gacgaagatg tttcaggaat cagaatatct
    32581 tcagcaccaa cagcaatacc actgcgcaaa gcgatgaaac cagcatcacg tcccattact
    32641 tcgataaaga aaagccgttc gtgcgaatca gcggtatcgc gtattttatc aacagcttcg
    32701 acaaccgtat tcaatgcggt atcgtaacca atacaattgt cggttccggc taagtcgttg
    32761 tcgatggttc cggcaattcc aatcacttta atgtctttgt attgtgccgt aaattcatat
    32821 gcaccccgga atgttccatc accgcctata acaaccaatg catcaatgcc agcatggcgc
    32881 aaattattgt acgcgcgatc catgccttct ttggtcataa aatccttact gcgtgcggtt
    32941 ttcaacatag taccaccgcg ctggataatg ttggcaacgc tttttgtttc gagttcaatg
    33001 aaatcgcctt ccaccattcc ctgataccca cgccgaatgc cggttacatg aagaccatga
    33061 aaaatagctg tacgtgttac cgcacggata gcggcattca tgcccggcgc atcgccgcct
    33121 gaagtgagga ctccgattcg tttaatctca ttcattcctt ttctgttttg ctaactacaa
    33181 aggtaacaaa agcgaaggga ataaagaaat cggattgtag aaaacagctg atgtttttat
    33241 ccggcaacga cagcatgaat ataccccgcc acctcagcag cggaaatttc tcgcatacac
    33301 gcacaatgag cggttttgcg gcagtccgaa cattctttat ccataacaaa tactttcgtg
    33361 ttttttccaa tgggcgccca acgaccggga tgcatggggc ggatgggtgg atacaggccg
    33421 atggcatgaa tgccgagtgc agctgcaata tgcagtggcc ctgtactggt agctaaaagc
    33481 gcattgcaat gctgaacaaa aacaatcagt tcacttaaag acaatttacc ggataaatcg
    33541 gtcacctgtg gaaatggctc tgcaagctcg ctacgaaaca aagctccttc tttttctgta
    33601 ccggtcagaa agattttgaa tttatcggct ggcagcagtt tcatcagctc cgcgaagcga
    33661 tcgattcccc attcgcgcgc gctgcctctg gagcccggat gaagaatcag attgaattta
    33721 cttttatcaa tgagttcatt caaatcaact ctcagtatgc tatttggcaa caacaactgc
    33781 attgcgttca attcatccaa tgaagggaca tgagaaatac caatgccttt cagcaattca
    33841 aaattcaatt gcgcttcgtg taaatcggaa ttctttcggg tgaaactgac ttttcggttg
    33901 cagttgcgcc agtgaaaaat acggtgagat gttcccacac gcagctgtat tccggctttg
    33961 agtgcggcct ttgcgatttc cttccgcgga aaaacatgaa taatggcatc ggcattcaac
    34021 ttccggagcg attctgcagg attatccgac atatcatccc agctaacaaa ctcatcaacc
    34081 tcagaacagc actgcacaac agcactggta taattacgtc cgagaaaaat aacagtacaa
    34141 tcctgaaaac gttgtttcag aaaaacagcc agcggcaatg tgagaattac atcgccaatg
    34201 gcatcggtgc ggctgattat gattcttttc aggttcataa acggaaactc aaaagtaatc
    34261 aaataactta aattccgaaa tgtctttgcg aaatgaacgc attaaaattc accaagcacc
    34321 tctgaaatcg cagttccacg gagtaaacta tgcaaaaacc gaaataggaa aacacaactg
    34381 cattgtgttt catcaaatac agcaaattgg tttctgaaag ctcgctttca aactcacttt
    34441 gctgtatttg atatcggctt ttgcatcgcc aataacactt tgtgcaactt cgtgagcact
    34501 taatgtaact ttgtgctaca ggctgtttga ggcattcgca acgctgagag gtcgctgcgc
    34561 gacgcactca ggttgcgtga gtcaatcatt gaattcgtct tcggaaaaat cgccggtact
    34621 taattcagta tctctttcaa ccgcattttg ggtcgggtac attccaagat aaaccacaat
    34681 cagtaacgca agcatgagtg cataaatcca tgctttttca acaaagaata acacgatgct
    34741 tatagtcatt gcggcatcga taaaagccag tctgacaata gcaacggata aatatgcctt
    34801 cattcgttct tctcttgatt ctttggagcg gacactgttg agcattatgc gtggaacaat
    34861 gaaagagccc atggcagagg taacaaccaa aatcaatccg atcagaaaca aatccggatt
    34921 ttcatcattc atccagatca ggtctttgtc gatgtttaca aaaaacacga tgacaaaaaa
    34981 aacaagtgcc tcaataagta tggcaatgtg gataatccgc agcgttttga gcgatttcat
    35041 tacggcagga ttatggcaca agaacttcgt aagtatatgt cagcaccttt ttctcgccgc
    35101 ctttaatgct cagatcccat ttgatttcgg aaagcggatt taccccataa tacgaaccgg
    35161 tttttttact cacgcctgca tccgaagact ctgtaacggc agcatatatg gttttgtcga
    35221 ctttcagatt tatgactttt tcctgaagat tctcaatttt aatttcgcct ttaatgacca
    35281 cttttgcata tttggttctg ttgattgttg tatagttatc agtgcgtgat tgttcctctt
    35341 cggtgtatgt gaccacaatg tcgctcgaac gtgcaagctg cacgctggtt tcgcctttca
    35401 ccggcgtgta tttaagctga tcctgcgcaa gtggcatcag gttttcatcg agcacaaaaa
    35461 caggacctgt tgtaaatggc tgtgttgttg catttgtcag gcgcaagcta tgaaagacgg
    35521 tctgacgatt cttctcggtg tccatcacat agcggtttcc ctgataattc accagatcgc
    35581 cgatgctcac ttcgtaaata tccttgtacg tagtattctg cgaaaaaatc atcaccgatg
    35641 tcttggtgtt tttcggcaac gtcacttttc cgcatttgta aaaatacagg tcattcgatt
    35701 tttcaccacc ggtgttgtag ttgtaataat tgttgtaatc aacgccaccc gctgttgatt
    35761 ccgacatggt catatcgtcg gtctgaacct gatagttgtc gaaccgggca ttcgaaaact
    35821 gattataagc tgtgccacct gcaccccagc catatccggt caggtatgat gttgcaatgg
    35881 gatcgagctg agtaccataa taaaaattcg gactacctac agtcagcacc agatcggtgt
    35941 ttacaacatc atcttccata ttgttttcaa ccgttgcttt taattcaagc tgcacttcct
    36001 tatcgttcag aattttgatg ttgtagcaag gagtccactg aataccgctc tgcatatacg
    36061 atacccgcac aggaactgat ccggactgtt tgctgaatgt tacaaatccc ctacgcacaa
    36121 tgctgtcaac ttctgctttt tcaatcggat tttcatcaaa agaaatttct ttgataaacg
    36181 acgaagaaat aaatgtagtg cggttatcag tgcctttcac tttcagcgtt gatgccgatg
    36241 tattgaagtc agataaagtc cctgacagtt ctcggatgtt cttttcatca agcatgtaaa
    36301 ccacgcggag tttcttaccc acattggctt tgagaagttc attgtatgaa aagaaatttc
    36361 gtttgttttt cagtgtgtcg tttacaatac ggatttgttc aattttcaca tccttggtgg
    36421 tcgatatcca gtaagtgctg agcagcggac caggcggcac cattacggat gcttcgtaat
    36481 tcgaaatgct ggcactgcct tcgcgcatga taaagtaagt accattttta aacacgttga
    36541 gctgcgtggg cacagtagtt tgtgcgcttg caacaatggc tgcaaaaatg cacaggacaa
    36601 gaatacggat tttcatgatt tttattttat gctaagataa taaatcaaac aacttctata
    36661 aaatgaaaat cataatagaa ccagaaattt tacagaaaaa agaattgcct gaccatggcg
    36721 tatttcagac aagaccaata tccgtgaaag caacaatcat caaaatcctg acaagaattc
    36781 agcaatacaa agtcatggca agagccaaag aaaaatcgtc ggataccgtt ttaagattca
    36841 gctgattccg cctgaattcg tttccctgta aaccgggaat tcagcaatcg cgcaacaccc
    36901 atataatcag gtgtaaaaac aattgtatca ccgacgtgat attttttaga gcccttttta
    36961 tcacggttgg tgcccacatc aacaacagtc atatccgacg ttatgccaac aaatttcaca
    37021 gatttatcaa taggtttcag actattcata tcaacatcaa taagtccgaa atccagaatt
    37081 gccttaatgg ttttgcggct tctgccggaa gaatcaaaat caatggtatg tccaacattt
    37141 ccatcagaga taataccatc cggcacccga tctttctctg ccagttcaat aatatttgca
    37201 tagtattcaa acgtattgcc attaagatcg cgaaaaagct cattatttaa agggctggtg
    37261 ccaaaaaatg cagcttcacc aatccgaaga tgattgatat cagcaggcat tttgccagca
    37321 tcgaccaaag gcaaagtaat tgagctacca cctgaaagga ccggtaagga tttgttgaag
    37381 cgcaattcga gaacctgccg atacaatgac aactgaagta gtttatcata agttggctcc
    37441 actccataca tacagcccag attggttccc aacccaatga cttcaatatt gtcaagatta
    37501 aaaacgcggt tatagaaatc aaccagatta tcacgcaaaa caccttcgcg gagctccccc
    37561 aattcaacca tgataatgac ctgatggcgt ttcttcattt tttttgcaaa atcatttaac
    37621 ttaacaatag tgcgcaacga agaattcaaa ctaatgtcgg cataacgaac cacgtcttcg
    37681 gcataagcag ttgcaggagg cttgatataa attgttttgg catcggaaca aacctcttta
    37741 atgtttctca gggacgaaat tcttgaatca ccgacagaat ggatgttttc aatccgctct
    37801 ttcgacaaaa tatgttcaag caattcccgt tcgccggcaa aaaccttggt aataagactc
    37861 cacataaggt tgcgcgactt cagaaaatga ctgattgaat tgatgtttcc agttatcctg
    37921 tcaatattaa tatttaattc tgccatatca actcttttta aaacgcatat ctgcatattt
    37981 atttttaaaa ccaattcttt cgtacaagcg tttggcgggg ttatcatact ccacatgtag
    38041 tttcacatcg ccatcacaca aatcaattgc tttctcaatc atcattttac caataccctt
    38101 tccacgcatt tgagaatcaa ctgctacata aacaataata ttctccggga tataaccttt
    38161 catacctgtg cgattaacaa ccaaaccccc gataatttta ttgttttcaa tcacagaaat
    38221 tgcaaatccc ccagctttat cattgtcaag caaagcgtaa tcaatgcatt tttcaatatc
    38281 ttcaatggga tctccaaatg gctgcaaata agtaaaaaga aaatcgctga aatctttcct
    38341 tgaaaaaggt tcagtaaaat cagacatttg tttgataatt cttgtttcca tattacaaaa
    38401 atttattgaa cacgtgtaaa ctgtgcacac atattcatgg ttaataaaaa tcaaggatca
    38461 aacagcaaaa aaagctataa acgtgatccc tgtacaacaa aacggatgca aaacatcaca
    38521 tttttaaaaa atcggcaaaa caccgcatct acaaatatcg gaaaaacatc ggaattatac
    38581 aaatttttaa cacttgtcta acaagtccga ttgtgaattc caatgacagt tataacacct
    38641 ccacactgca taaaatcagc actttcggga agcacagatc aaaaacctca aattacccga
    38701 tcgacattct taaatcatca cgtttcttct tacttcgaaa ttccttgcct gcctgactgc
    38761 ctgttgcagt taaactggta gacaggttcg gtgttcatca ctcttccatc caatcatcca
    38821 actctctccg gtcttttttg gtggggcgac cagtaccttt tggacgttgc agcacgaggc
    38881 gttcgcggac catgtcgagt tttttgaatt cttcttccgg tgttaattca agaatataat
    38941 tcggcagcaa cttggctccc acacgcgatt tcggaaagtc aacaatctga atggtgcgtg
    39001 tgaatgcatg cagctttatt acaataatat ctccgacagc cacatcccgt gcgggtttca
    39061 cttcctgatc cttaatactt acacgaccgt tgcgacaggc ctccgtagcc agtgtgcggg
    39121 ttttataaat tcgaacgtcc cagagaaatt tatcgatgcg cattgtatag aatatgttgt
    39181 tagctttctt tagtcgttac acgttacaga cgttacaatc gttacgcgtt acaatcgtta
    39241 cagtcgttac aagggtaaca aggtaacagg gtaacagggt aacaacgtta cgcgttacaa
    39301 tcgtttcaaa gcttcactca taacccataa ctcataactt ataaatcacc aataactgtt
    39361 caccgctcac tgttcaccgc tcactgctca ccgctcactg ctcactgctc actactcacc
    39421 gcttagtagc agcctgcctt gcacactcag ccctttttat ttttcgcgaa aaaacagctg
    39481 aatctggcaa cctttcaggt caaaattttc gcgcagtttt ctttcaacaa atcgtctgta
    39541 cggctctttg acatactgtg gcagattgca gaaaaacaca aaagttggat aggccagctg
    39601 caactgtgtg atgtatttaa tcttcacacg cttcccttta tacatgggtg gcggagtttc
    39661 ggtaagaatc ggcagcaaca cctcattaag cttcgatgtt ggaattcgcc tgccgcggtt
    39721 ttcatgaacc atctgcaatg cttcgagcat ttttatcacg cgcgtttttt ctgtggcgct
    39781 ggtaaaaata acaggcacat cggtctgcgg catcaggcgc gcaatcagat tttccttgta
    39841 ttcttccatc gtcttatgat ctttcggcac cagatcccat ttattcatgg caatcacaat
    39901 tcccttgtga ttttcgttga tgatattgag aatatgcaga tcctgtgctt cgaggcctct
    39961 tgtagcatca actaccaaca gacagatgtc gctgttttca atggagcgga tggctcgaac
    40021 ggttgaataa aattcaacat tatcgctcac ctttgctttt ttgcggattc cggcggtatc
    40081 aaccagcata aaatgaaatc cgaacttgtt gtactctgta aaaatgctat cgcgtgttgt
    40141 acctgcaagc ggcgtcacaa ttgacctttc ttcgccgata agcgtgttca ggattgagct
    40201 ctttcctgca ttcggtctgc cgacaatggc tatgcgagcg acccccggat ccggctctgc
    40261 ttccatcgga ttgaaatgtt ttacaacctc atccaacaat tcgcctgtac cagcgccatt
    40321 tacagcacta attgcaaaaa tttcgctgag tccactggaa taaaactcgc caatattcaa
    40381 tgcctgagca ttgttgtctg ctttatttac agcaagcaca accggctttt tgcatttgcg
    40441 cagcatgtcg gctacatctt cgtccatcgg tgtgaatcct tcgcgcacat cggtcagaaa
    40501 caaaatcaca tcggcttcgg aaatggccag atgcacctgc tttttgattt cctcttcaaa
    40561 aatatcatcg ctgttattca cgtatcctcc ggtgtcgatc accgaaaaat gcacaccgtt
    40621 ccagtcgctc ttaccgtaat ggcggtcgcg tgtcacgccg ctggtcggat cttcgatggc
    40681 tgcgcgctca ccgacaagcc ggttgaaaaa agtcgatttg cccacattcg gacgccctac
    40741 tatggcaagt atgttactca tatcaaaatc ccatttgttt taaagttgtt tcatcatcgc
    40801 gccagtcctt caccttcaca ttcaaatcga ggaacacatg ttgtccaaga aattcctcaa
    40861 tatgtttgcg cgaaagtgtt ccaagttttt tcacagcact tccaccctgc ccgatcagaa
    40921 tccgcttctg gctttcgcgg cttacaaaaa tcgtggcgcc gattttcgga atgttttcgt
    40981 cttccttata ataatcaatg attacctcgc agtgatacgg aatttctttc tcaaacagat
    41041 aaaaaatctg ctcgcggatc atttcggaaa cgaagaaccg cagatgtctg tcgctcagca
    41101 catccttcgg ataataagcc ggatgctcgg gtaacaattc aatgacacgt cccagcagca
    41161 catcgacatt gaaattgtga agcgccgaaa tcagcatcac atctgctttt ggaaagacca
    41221 cttcgtattg tttcatcagc tccatcatct tttcctgctg actcagatca attttgttga
    41281 tacagacaat gagcggaact gtcaggttct gcaggcgcag cagcagattc ggattgagtt
    41341 cgcgtgtgcc cggttcaatt aaaagaataa cgatatcggc atcctcaaac gcagcattga
    41401 tatatcccaa catcttgcgg tggagctcat aggccggatt ttcgacatag ccgggggtat
    41461 cggaaaaaac gatctgataa tcctcggtgc tcagaaaacc gagtacacgc tggcgtgtgg
    41521 tctgcgcttt tggcgagacg atggaaattt tttcacccgt caaagcgttg atcagcgttg
    41581 acttacctac gtttgggttt ccgattatat tgacaaatcc gcttttgtgt gacatttttt
    41641 tgccgggtat taaaattgtt gtatctttgc agtcccgaat tgcaaaggta agcaaaaaga
    41701 actaaagata tattgcgggg tggagcagtg gcagctcgtc gggctcataa cccgaaggcc
    41761 agaggttcga gtcctctccc cgctactaaa aacagagtga gaatcagagc gaaagcgaag
    41821 attctcattc tgtttttctg ctcactcact ctcatactct cactaaatgc atggaatcag
    41881 agcgaaagcg aagattctca ttctgttttt ctgctcactc actctcatac tctcactaaa
    41941 tgcatggaat cagagcgaaa gcgaagattc tcactctgtt tttctgttca ctcgctctca
    42001 cactcacact aaatgcatgg aatcagagcg aaagctcatg agtgccgctg aaaatgatat
    42061 aatgaacgaa taatctgcgg ataaagaaaa gggtgatgct tcgaaggttt tccaagagat
    42121 cccggcacag tcagcaaagc tgtgcctcag tcagcgaccg ggaactcagt ggcccatgtt
    42181 gaatatgatt tttgtacaac ctgaatcagt ctttaccgct ggcctgcctg actgcctgtt
    42241 gcagttgaaa ttgcagacag gtgaaggcta attcgcggaa gaaagattgg agatgcggcg
    42301 aaggttttcg gtgagatttg tgaatccatg tcacggatga catatccgcg ccatggttgg
    42361 ggtaaacatg tccgactgcc ggcggattcg cgaaagaata attgaacatg cttcgaaggt
    42421 tttccaggag atcccggcac agtcagcaaa gcggtgtctc aatcagtgac cgggaactca
    42481 gcgcgccatg ggagatttct gcggagaatt tcacccgcaa gccgtagatc atttcagccg
    42541 ggacatcagc aattttgaat tgtttatata agattcgcgg ttgtaaaatg aatattcact
    42601 aattttaccg caatattata atttgcaaaa tgaaaacttt tcttctttta tctgcaatga
    42661 tgtttgtctt cattgcatcg atggctcaga aatcggaatc cgacaaagac tgctggtaca
    42721 acaaggacat ggagaaaaac aaggtcccgg gcgtttcttc ggatcgcgcg tatgatgagt
    42781 tgttacaggg caaaacaccc aaaccggtga ttgttgccat tattgacggt ggaaccgatt
    42841 tcaatcacga agacctgaaa gaagtgatct ggaccaacga agatgaaatt cccggcaacg
    42901 gaatcgatga tgacaagaac ggatacattg atgacattca cggctggaat tttcttggaa
    42961 atccgagtgg cgaaaatgtg gacaacgaca accttgaagt gacgcgcgtt taccgcctgc
    43021 tgaaaccgaa atacgaaaat gccgactcgg cgaaaatggc cagtgacgaa gaatttcagc
    43081 tatatctgaa atgcaaaaag gaagttgaaa ctaaatatgc tgcttcaaca aaagccctcg
    43141 ccaatgtaac tacactgtat cagaacctga aagtagcaga cagtgtattt actgcctatt
    43201 tcggaacaaa taaatattcg gcaaaccaga tcaagaccat caaagatgaa aggatgcagc
    43261 cagtggttga tttcatgagc tattggatga aaaaagatgt tgacacaaca atcgtaaaag
    43321 ggtggattga tcattatgaa agtcaggcgc tgtaccgcta caacgttaat ttcgattcac
    43381 gtaccattgt aggcgacgac tggactcaaa acaacaatcc gtattacggc aacaacgatg
    43441 ttaaaggccc gcgtgcaggc cacggaacca tggttgcagg caatgtggcg gcagtacgag
    43501 gcaacggtat tggcccggat ggagtgacat cgaatgcgca gttgatgatt ttgctggtgg
    43561 ttcccgatgg cgacgaacgc gacaaagatg ttgcaaatgc tatattatat gcagtaaaca
    43621 acggtgcgtc gattatcaac atgagtttcg gaaaaggata ttccccgcag aaacattttg
    43681 tggattccgc tctgcgtgtg gccaacaaac acgatgtact gattattcat gcggcaggaa
    43741 acgatggcga aaacgtggat gttgaaattc attatcccag caatttcgat gagaacggca
    43801 acataatcaa cgacaaattc attgttgtcg gagccagcac caattcagcc ggaaagaaac
    43861 ttcccgcatc gttttcgaac tacggaaaaa agagtgttga catattttca ccgggtcagg
    43921 atatttacag ttgtgcaccc ggcaacaaat atgaactggc ttcgggaacc tcgatggcaa
    43981 gcccggttgc aacaggtgtg gccaccatca tccgcgcata ttttcccgag ctgaccgcaa
    44041 aggaagtgaa ggaaattttg ttgcaaagcg ccgtaaaata cgacagaaaa gtcctgttac
    44101 caggcggcag caagaaaaaa gtttctttct cagaattatc agcaaccggc ggagttatca
    44161 atgcgtacaa cgccgtgaaa atggcacagg aacgcgtgaa tgcaaagcat taatcccggt
    44221 cttccgcgaa tgacgccaat ctccgcgaag aatctgatta attggctcac cttcggcttg
    44281 catatgaacg cgaatagtta taaaaaactg cttcgcaatt ttttataaca aagccgtcaa
    44341 gatgctttga tatttttttc acgacaagat aacacgtttg gacatcaagt ttttgcaata
    44401 tgcagcagga tgtaaaacct tcacaaaaac cagtattatt catcctgcaa tgaagttctg
    44461 aaaccaattc tctttctgtt ctggtaatct gaatcaattt ccttcgcttt ttccagttga
    44521 tcaaggtatt caaaaatcaa cgcgagttgt tgatcatgac cgttagcttt tacatcaagc
    44581 atttcgatcc ttttcagaat ttcttgctgc acattaatca tttcgcgcat tttaacgaac
    44641 acttcaataa tcctgatact cattttgcga gcaatatcac tccgaagcac atttgccaac
    44701 tgcaatatgc catattctga aaaggcaaat ggcaaatatc tggtgccgcc ccaacttgag
    44761 gtcacagttt gtgacctcaa gttttcgaat tcctctttgc taagttctat catgaaatta
    44821 tccgggaaga tatcaatatt tctcctgact gcttgtttta gagcttttgt ggagacctga
    44881 tacaaaatgg ccagatcaaa atccaacata acttttcttc ctctgataca ataaattctt
    44941 gtagcagcgg attcatctga atattctaaa gctgaacttc tgtcatcatt tttagccata
    45001 acgctatctg tttagttgtg acacataatt tatttattaa taccggtctt ccgcgaatta
    45061 cacgaatctc cgcgaaaact ttaattaatc cacttgcctt cggcatgcat atggacgcta
    45121 attgatattc tttttcagat aaaacaggat tcaagagccc cgaaggggcg cactacgtta
    45181 gccaggcccg aagggtctgg aaaaagaaaa atatgctgag ccctgtaggg gcgaaacgct
    45241 agtacaaata tagcgttcat catattccat ttgatttagt ttcagaaaat caagaaactc
    45301 ctcctgatag gtaattcttc tatgatgctc cttttgcgag tcgatataat cttttattcc
    45361 ctgcacatct cccggactaa cagaaaatgc agcataccct gattgccaat aaaaatgctg
    45421 atacttcctc ccctgggttt ttatccattg cgatgaacca gattttattt tttcaactaa
    45481 cttcatcagc aagacattct gtgaaagaat gcaaagaata tgcacatggt tcctgtatcc
    45541 gccaatcttc acaggagggc attccatgtt ttttgcaata cctcccaaat acgcaaataa
    45601 ttgctgctct atctgatcat caataaacga gatgcggact tttgtgctaa agacaatatg
    45661 cacataaagc tttctgaatg attgcgacat aatatgaaaa tttaatgcag aggccgcaca
    45721 tttttcaggc atagtgtttc gcactttgca aatatatgca gcccctgcat ggcatttgga
    45781 aattctaagc ggcctttttc atttcagtgg ccacgatctg cacgcggcgg tgagatacgc
    45841 cggcatcgtc cttccaggcg gagctgcgga gtttcccttt cagacgcaca cgagcgcctt
    45901 tgtgtacggt actcataaca acatcggcta ccttattcca tgagacaacc tgaaaccatt
    45961 ccgtgatgcg cacctgctcg cctgtcctgg tgcgataatc attaaaatat gacatcgtga
    46021 aaagcgccag cttgttgcca ttcgcaaatt gcttcggaaa cggctcagag gtcactacgc
    46081 cttcgagata aacaatattc ttttcaggat ttcttttctt cttcggttct tcttcacctg
    46141 taagcaccaa catgcgggct ttttcatcat ttgcgttcac gctttcaaac tgtaattcca
    46201 taatcgtttt aatttttagt tcaactgaag ttcgccgttt tcatcaacga caatgcaaag
    46261 gaacaaccgc cggaatccat tagctggtta ttaagcattt actttcgact ataaatgttt
    46321 gtaaacgttt gttgtcggct acaatatgtt cctgattaca taatcaggaa cagcgtcatt
    46381 tcacattatc agtgcagaca ataatttttt tcaacaacaa gtacaaaaag cctcatttct
    46441 acgtacaacc cgaccttcca gagtccacaa tgcaatggag aacctggaag agtcggaagc
    46501 tgtgaactct tctgcacccg acgactccat atcctctgcc tacttcgtcg ccatcgggag
    46561 tttatcccgc cacggcggga atgcaatatt atgattgccc gtcaatcgtt ttcttcgctt
    46621 cgcagaaata ctgaaaatca ttcacgtttt cgaaacgcag catttcaaag cccgaatcgg
    46681 aaaaatattt ttcaatggtc tctctgctca gccgctcatg caacggaggt ccagtgggcg
    46741 attcgacagg cgcccattca acaatgaaca attttccatc ggctttcaga atttgccgga
    46801 gcattttcag ataggcaacc gcatcgtcca cttcgtgaag cacaatagcc atcagtaatt
    46861 tatcaacaga cttttcggga agttgaatgt tatccttttc gcacaacagt gttttcaaat
    46921 taagcgatcc gggagcatca gacataagca aatccagcat ctgctgcgag atatcagcgg
    46981 ctatcacatt cccggtatcg ccaacatatt ccaatgccgg aatggcaaag tatccttttc
    47041 cggcgcctgc atcgagcatc acatcgcctt tttgcaaatg catcatgtcg agaattttct
    47101 caggcggtat gagcttacgg cgctcagcag aacttagact ttcatgcttt tcaggattga
    47161 atattttcga cattccaagc gttttagttt agaaaataaa atgcagcatt cagtgcagtt
    47221 gcaaacagaa tccatagtaa atacgggata ttcagatagg cagcgatggg tttcaatcga
    47281 tagaaattca acaacatgat aacaacaaca acatcaagca caatgatatc gataagtgcc
    47341 agcgcgatat tctgataata gaaaaacaaa aaagaccatg caaaattcaa cgccagctga
    47401 atgagataaa taattttggc aacagtcctt tctttagtcc tgggcatgat ccagatataa
    47461 aaaaacgaaa gtcccatcaa gatgtagagc agtgtacaaa caggtccaaa aacccaacct
    47521 ggcggattaa agactggttt attcaatgaa gcaaaccatc cgggaaccac ttcggatgtg
    47581 tattgtccgg caatatagcc cagtgccagc gggacaaaca gcgaggtgaa aaatttaaaa
    47641 atacttcgga gcatacagtg agatattttt aggtcaaggt taaggtcaag gttaaggtcg
    47701 aggttaagta agactttcgt tacacgttac agacgttaca atcgttacaa tgttaagttt
    47761 gaggttaagg ttaaggttaa ggttaaggtt aaggttaagg ttaaggttaa gcacctttgg
    47821 tgaatctggc atgttttcaa aaaaaatctc tttctacatt caacagcaat ttccaatttc
    47881 agtgttcatg attcaatgtt cggcatgtaa tactcccttc atccttcctt gttcctcttg
    47941 atccgtcaga atgcacagat tgttcagtgt tcattattta tcattacttc ttccttcact
    48001 tcgaaaatca aaaatctcaa atctgatatc gtatatcctt cggctcactc ttcttcccat
    48061 tcaatctgga atttcgtgtt ttctttcggg gcgggttcaa cctttttgat gcttgtgtct
    48121 tttttgaaaa ggccaaattc ttcattcaga atttttttca gattctgctt ttcggctttc
    48181 agatcctgtt tcactttttc ctgcatggcc tttttgtcaa gctgcttgta ctgcgggttt
    48241 tcgacggttc ctgtaatcag aataaaaata gtcggatcgc ccactccatc atcaacaaca
    48301 taaccatatt catcttcggg acgggtttta tctccgcctt ttttcacatc cgaaaacttc
    48361 acttcgagat gatattcaat gttattttca aatgtatgcg atccggtaac cttcagattc
    48421 attacgtttg agcgaatttc cgttttcgga attttaatgg ttttgtcgct gatggaaatg
    48481 gtgctgacca ttttttcaaa ctgaatatcc gaaaaatctc ttcctttaat aatcttctcg
    48541 agatcgttga tgggcgcgta tcctacaagc tgtccatctc tgatttcaat ctcagcacct
    48601 gcaacaatcg atgccggatc aacttcgagc gtttcggaaa aagttgcttt catctgcagc
    48661 gaaacatctg tgcggccacg cagattgtca taagtcagat ctttctgtcc gaaattattg
    48721 aacacaataa atgcttcacg cacgtcaagg tttttaagat ttgcatcaag cgaaacctta
    48781 acatccttgt ttttaagctc acaggagccg cttcctcgaa tggatccacc caatgtcttc
    48841 agactcacat cttcggccag aactttgttt tcgcccacat gcaccgtggc ttttacattg
    48901 cttgcaccga atttattata cgacaaacgg tcggccgaat actctacaac cgctcggaca
    48961 ttttttggta aaacaaattt tatttctgtt tcttttgtct tctgcgtatt ttcttcttca
    49021 gggaaaagct ctgtgacatc aatattcttc gaatgcagat cagcactcag tttcagctcg
    49081 ttgtcatcaa taaacagaaa tggcagaatg tcctttagaa tggcatttcc gctgaaatca
    49141 ctggaaccat atgcaacatt catttccttt atttcaaggt cacggtttga aaaaatggca
    49201 tccagttttt tcacagcagc cactttgctc attccttcgg tctgcagctt tgcatcttcg
    49261 atcagaagcg ttccctgact tttgctgtca ataaaatcct ttgcctgcaa attggaaaaa
    49321 tcgtcaatgg ttccttcata ctgaacatca aatttcacgt tgcccgccat ctctttcacg
    49381 ctctcccact ggccccattc ctgaattttt cccaaagaca tatcgccttt caacgacaat
    49441 tttatctgcg gatttttcag gttcataatt tgcagacttc ccgaaatggt cttcccgtcc
    49501 agtgttgcac tgaagttttt cattgacagg aaatactttt cctgcgcagc aaaatcgggt
    49561 gttgtaaatt ttccgttgaa gcgaatgttg ctcagtgcaa tattgttttc tggtctttca
    49621 atatagccat cctgcatcag cagtgacaca tcgactgagg gcattttccc gtcgctgtac
    49681 tttcctttga tgtgcgactc gattgaaata gtaccgcgat gctcgagtcc atccgagaat
    49741 ttccggactt cctgcggcat cagactgatg aattcattca gatcggtgtt gtccgatttc
    49801 actgtgatat caacggtttc gcgcgattta tcataatcca ccttccctgc tgccagcaga
    49861 accacatcgg caatctgaat ctccacgcta ttgaatgaaa cattcgaagt cgatgtgttt
    49921 acatcaatca caccattgag cagggcgtct gttccgggaa tgaagtccat gcctgaaaca
    49981 tggatagtgt caacacgcaa tgcagcagac agtttcacgt tgtaatcatc ttcgtaaaag
    50041 ctgcccgatg ctctggcctg atgaaaaaag aatgtgtatt gctgatcgaa acgctcatca
    50101 aaatatccac cacgcacatc ggaaagatag attctttcaa gatgaaacgc tgtactttcc
    50161 gaagaagtgg ttgtatctgt tgttggtttc agaatatgaa aattatcaga accatctttg
    50221 aaaacccgca gccagactga tccgcgatcc atcccaatct ggcggatggt gtagtcacca
    50281 gtaatcatat ctatcagatt gaactcaagc gaaacccaac gtgctgaaaa aagatttcct
    50341 gttttctgct gtgtggcatc gctggccgac acttcgttga acactaccga caccatggga
    50401 aaatgatgaa acaaagtcac atcaacatcc tttaccttga tttcggaatt gagttccttg
    50461 ttgatctgac caattacttt ctgctcaatt ttattctgaa aaataaacac cagcagccat
    50521 gccagcagaa tcagaaacac cacggatccg atcagccaaa ggaaaatccg cagtgcgatt
    50581 ccgtaccatt tccgcttttt tctggtcttt gtttctgttg ccggttgttc catctgtgta
    50641 ataacgttgt tacagctgta aaaattacat tgacttttcc gacctgaaac tgtcccagcc
    50701 ctgcgccttg agcgaaatac tctggccggc tttggtgatc agattcatgc cttcgttttt
    50761 gtcggtgatg tgaccaatca ccagaatgtc atcgcggtct ttcagctttt cgtattctgc
    50821 cagcggcacc gtgaaaagta gctcgtagtc ttcgcctccg tgcaatgcca tggtgatatc
    50881 ggtcacattg aattcttcgg ctactctgcc tgtttcaata tccaccggaa ttttttcctc
    50941 gtatatactg catcctgtgc cgctctgcga gcagatgtgg tgaagctccg acgaaagtcc
    51001 gtcggaaata tccatcatcg aagtcggttt aattccaacc ttgcgtagtt tctccatcac
    51061 gtcgaaacgc gcttcaggtt tgagctggcg ttctatcaca tattcaaatc cctgcaggtc
    51121 gggctggaaa ttcggatttg cctcgaaagt ttttttctcg cgctgcaaaa tgagcagtcc
    51181 tgcgtaggcc gctccaagat cgccactcac gcagatcagg tcattcacct gagcaccgtt
    51241 gcgataaaca atgtcttctt tttttgcttt gccgacaacg gtaaccgaaa tcaccagtcc
    51301 ctgagcgctg ctgctggtgt cgcctccaac gagatcgacc ttgtatttgt cacaggccat
    51361 tttcattccg gcaaacaatt catccagtgc ttcaaccgaa aaacggtttg atatacctat
    51421 actcaccagc acctgttgcg ctactccgtt catggccgca atgtcgctca gattcacaac
    51481 aatcgatttg tatcccagat gacgcagcgg catccacgac agatcgaaat gcacaccttc
    51541 gagcagcaga tcggttgaca ggagggtttg ttcttcagcg tcgtattcaa tgacagccgc
    51601 atcatcaccg atgcctttgc gggagctttt gttcaccagc ggaaaatctt tggtgaggtg
    51661 atgaatgaga ccaaattcac ccaaggtgga aatatcggtg tgggccggct ttttatcttc
    51721 gagcatagta ttcctttttc tgcaaaatta atcaatcctt tacctttata aaagggagag
    51781 aggagatgtt attaaccgca gaggtttgaa aagcgcggtg ctaattggat tgttttgaga
    51841 gtcaagcatg ctgaatttat tcgtcagcat actataacag agattttggc ctccgagccg
    51901 ggatctcatc gaaaaccttc tatgccaatg ctggattgta cgccgccaaa actgtttcgc
    51961 agccgacgat tccatgtcag caagctttgc tgttaatccc cttcttgctg acgatagaat
    52021 gtcggcgagg ctgcatctca gccaacgctg gaatgttctc cttcgtcgga ctatggattg
    52081 ttggttttca caacagcttt gccgatgaac aaatattttt tgcggtcgtc gagacgctga
    52141 cataaaacgc gcgtgcgctg cagactaagt ttgcgataag ttttttcttt ccagatgaat
    52201 tcggcgccgt cgggtaaatc ttttacgcgg gtttcacctt cctgaatttc gatttcagca
    52261 tcactgtttt cgtaggtcac gcgcggcttt tcgagctgat acagcgcttc ggttttccat
    52321 tcgggtgcaa attcatacaa cgacatgaca atacgcagca acattttgaa ggcgaatttc
    52381 cattcctcgc cgtgcggctg agcacggtgt ccgtattcgc gccacaccat ggcatgcgcc
    52441 agttcgtgac acagaataat cataaaatgt ccaagtggca attcacgtcg cacatacacc
    52501 cgcgccggaa tatcatttcc tttcaccatg aaagtgccaa gccggtgttt tgagttgcgt
    52561 tgaaaataaa tttcggtatt gagccgtgtt gcagcatcgt gcaaaaacag gtacaactgc
    52621 cggttgtaat tttgtacaaa aaaagttgct gaaaccggtt tcatattccc tccggaaaac
    52681 ggaattaatc aagcttaaga acagccagga atgcgctctg cggtacttcg atgttaccca
    52741 cctgacgcat gcgtctcttt ccttttttct gtttttcgag caatttgcgt ttacgcgaaa
    52801 tatcaccgcc gtagcatttt gcagtaacgt ctttgcgata tgccttgatg gtttcgcgcg
    52861 caatgatttt ggctccgata gcggcctgta tggcaatgtc gaactgctgt cgcggaatca
    52921 ggtctttcag tttttcgcac atgcggcggc cgaaagagta tgaattatct ttgtgaatga
    52981 gcgttgacaa ggcatcgacc tgctcaccat taagcaaaat atcgaggcgc accagatccg
    53041 cttcgtggaa accacattca tgataatcga acgaggcata tcctttcgaa atgcttttca
    53101 gtttatcata gaagtcgaaa acgatctctg caagcggcat ttcgaatgtt acctccacac
    53161 gatccgttga aagaaatacc tgatttttca ttacgcctcg cttgtcaata caaagcttca
    53221 tgatcgcacc ctggtattcg gacttggtaa taacgtttgc agtgatatag ggctcttcga
    53281 tgtaatcaat tttggtgata tccggaagtc cgctcgggtt gtgcacttca acaagttcct
    53341 tcttcagcgt gtacactttg tacgatacat tgggaaccgt cgtaatgaca tccatgttga
    53401 attcgcggtc gaggcgttcc tgaataattt ccatatgcag gagtcccaga aaaccgcagc
    53461 ggaagccgaa tccgagagca gcagaagatt ccggttcaaa tgtcagcgaa gcatcgttga
    53521 gctgcagctt ttcgatggat tcgcgcaatt cttcgtaatc atcggcatcg accggataga
    53581 ggccggcaaa gaccatcggt ttagcttcct tgaatccttc aataccagtt gtgcaagggt
    53641 tatcgacttt ggtgatggtg tcaccaaccc tgacttcgcg ggcgaccttg atgcctgaga
    53701 tgatatagcc aacatcgccg gccgagagtt cagatctggg ttccagcttc attccaagtg
    53761 taccgacttc atcggcgcga tattgttttc cggtgctgat aagcttcacc aggtcattcg
    53821 ttttaacagt gccgttttta atccggaaat aggtaataat tccgcggaaa gaattataca
    53881 cactgtcgaa aatcagcgcc tggagcggag cttcgggatc gcctttggga gcaggaactt
    53941 tattgatgat ggcatcaaga atttcttcga tacccatacc ggtacgggcg ctggcttcga
    54001 tgatgtcttc gcgcgggcag cccagcagtt caacaatctg atccttcact tcttcgggca
    54061 tggcgctggc catatccatt ttattgagga ttggaatgat ttcgagattg tgatcgatgg
    54121 cctgatagag gttggaaatg gtctgggcct ggattccctg cgtggcgtcg atgaccagca
    54181 aagcaccttc gcaggcggca atggcgcgtg agacttcata ggaaaaatca acgtgacccg
    54241 gagtgtcgat caggttcagc ttgtatttct gaccaccgcg ctcacagcgc atctgaatag
    54301 catgactctt gatggtaatg cctttttcgc gctccagatc catgtcgtcg aggacctggt
    54361 tctgaaactc gcgctcactc accgttccgg tgaattccaa taaacgatca gccagcgttg
    54421 atttaccgtg gtcaatatgt gcaataatgc agaagttgcg tatgttattc ataatcgggt
    54481 gcaaaagtaa taaaatacga gatacgaaat acgaaatcag aaatacgaaa tgatgtggaa
    54541 atgtgtcgct tcgctcatgt gacacctgcg gcgtatgtgc aaatatgaga ttctggaaag
    54601 atttgtaacg attgtaacgt gtaacgtctg taacgactgt aacgcgtaac gattaacttg
    54661 actatttcag aaaaaagttc aaagaaacga tcaaccccat attggtcagg tcaaaagtca
    54721 tttgctgctt tgtgtaggtg ttggcaaagc cggcattaat ttcattgttg tatccggcaa
    54781 tatttacatt ccagaatggc agcgtgtaat acggaatcag atcgaaactg aaaccgtctt
    54841 cctttccgaa tgagagcatg aaacgtacac ttccggacag aaaaaattta tcgactgcct
    54901 gcaaatattt atcgtagggg ccatttccga ttttatattt tacgaagaac tgatccatgt
    54961 tgaaactgaa tccgggacca gctctgaagc cctcaccatg aatcagattc agattgtaga
    55021 aaaagctgaa accatcatgc gaaattttta cttttctaaa atattcagcg tcgagtgaat
    55081 ccaaaccgct tcctttgctt ttgaagcgat gcagatgcaa gtcgaatccg taagagctcc
    55141 aaccattgtt tactttgcaa ccaagtataa ttcctcttga aaaaccagga gacccaagag
    55201 aattgtcggg agcgaataac ggctctgaat aatggttatt gaaatcggac acaatcagat
    55261 caatcggagc agtattctca tattgctcaa agctgtatcc gaaggtgaac aaagcggtct
    55321 gaccatgaga accgatgccg gcgaagaata acagggacaa taaaaaagct gttcttttct
    55381 tcatgttttc aaatattatc agaacaccat ttccggcgca atttcttccg ttttacggtt
    55441 ggaatgcatt acccgataac gcacataata ttcaaactga cctccaccaa caatgatatc
    55501 ggtaatcacc acatcgctgc tgtcgaattt cagtttaacg atatcgccga ttttatgtgg
    55561 aatcacaatg cgtgaagagt cgcgcgtata tccggcttcg acaagcccgc gatattttat
    55621 aagatgattg gcaattgtac tcagactgtc aatggttctg tttgccattg tcaaccgggt
    55681 aatttcctgc tgatatttca attgcatgtc ggccatggct ttctggtggc gctgattggc
    55741 agtcatgttg aaataaatga gcacgccaat caatcctatc attaaaataa ttccaatgat
    55801 tagccatatt ttagagctgc ctgtattttc ttcttgtgga atttttgaat ctgtactttc
    55861 cataaatatt attatttgaa caaatatata aatattttcc gtttcaaatt cttatttgaa
    55921 aatcatgatt ttctaatgag tttctacagg tttttgtttg tagtacttcg caacgcttga
    55981 tggcaaactg caaaaacaaa gtcggtcaga gttaccggtc aaagttttga atcatgaaca
    56041 aagaacaagg aaactcgaag tgggaattaa gtatgatgta aatttcctta atcattctca
    56101 cttccttgcc tgcctggcgt cagacaggtt cgttgttcag agttcgtggg gtgccgggat
    56161 ctcgtcaaaa acctacacag cccatgctta gacatcgacc acgtgaaacg tggccagaga
    56221 agagatcccg ggacaagccc gggaattcag cggtgatttc cgggctgtca gcaagaaata
    56281 ctcattcgcg tgatatctgt ctttgattta attaaaaatc gtttttgata ataattaatt
    56341 ttaattgcac aaaaaacttg catccaaatt tattaccttt acaaatcaat taaaaaaaac
    56401 atgaacagtt tcaataatct cccattgctg cagcaagccg acctgaaggg aaaagtggta
    56461 ctggtacgcg ttgaccacaa cgttgtaaaa aaagggctga ttcacgatcc gtatcgcatt
    56521 gatgcgactc tcggaaccct gtattatatt gtatcacgcg gcggcaaact gattctcatg
    56581 acacatgtcg gacgtccgaa agacaaaaag gcaggcacca ttaacatcgg cccggagagc
    56641 tcggttgaac ccatcgtgca gtatttcgaa aacaagctgc atgtgaaatt tgccattccg
    56701 gaatttttac ccgatggtga taaaggttac cgcggaattg aaacatcggt gaatcatctg
    56761 attcgcgatt tgcgcgaaga ccgcatcgac ggaatctatc tgcccaacac acgctggttt
    56821 tcgggcgaag aagccaaagg agaagatgcc gatcgctttg cctatcaact cgccggactc
    56881 gccgacattt ttgtcaacga tgcattcgga tcgtggcagc cacacgcctc cacagttggt
    56941 atcaccaaat atctgccgtc gtacgccgga tttttactgc aaaaggaaat gcagaatctc
    57001 gaccgcattt ttgcaccaca aaagccattt gtggctgtgg tagcgggctc caagttcgac
    57061 actaaaatcg acagtttgtt taccttgctc gaaaaatcgg attatctggt tttaggcggc
    57121 gtgatttaca atgcctatct ctgcgccaag tacggttttg aaatcaaagg gctcgaagcc
    57181 gatgatatca aacacgcaaa tcgttttgca gaatttgcag ctaaacatcc gggaaaactg
    57241 gtagagctgc ctttcattgt ggagtctgat acactcgaag gccgcaagga aggccagttt
    57301 cgcacgcacg atattcgcgc gctgaaacaa ggaacaaagc tcaattatgt actcgatgtt
    57361 gcgcgtgaat cattcagcga acaaagtgtt ctggacattt ttcatggcgc tcacactatt
    57421 tttgtcaacg ctgtaatggg cttcactcca cattttaacg aaggcactat tgcgttggat
    57481 gagttgattg ataaaaatat agatgccgta aaactctatg gcggcggcga caccatgcag
    57541 gaactgaaac gattgctgcc cggactttat atcatggcaa ttgacaatcc gaaatactat
    57601 atttttacag gaggcggcgc agtgttgaaa gccattgaac aaggcacggc atttgggctg
    57661 gaaccggtga aagccttgat gagcggtgaa cggtgagcgg cgaacggtga gcggttattg
    57721 gttaatggtt cttggtgagt tataagctat gagttataag tgatgagtta tgcatgaaat
    57781 tttgcaacaa ttgttacgcg taacgaaata atgtcgaaat gtaaaaaagt gtttctgatt
    57841 aaaatttcaa ttgaaaaaaa tgctgttgaa aaagttgacg ggttttgtgt tggttttgtt
    57901 catgctggtt tcatgcggaa tggcgcagaa acagggcgtt gaaacatttt ctattaaaat
    57961 cgattcgttg tatgatgaca acaatcagct gaatgctgca gggaatgcgc tggttgaaaa
    58021 aattgccgcg aaaatgaatt cgtttttcag cagttgtctg aatattaatc atagttgtat
    58081 ccatgaatct gacaccattt gctatcagaa gttaaagaca tttgtaaacg ctataattgt
    58141 gaaggttcct gctggccagt tttttggtcc tgtatcagaa cccgctcata tgagttgtga
    58201 agactgcttt gaattaataa taagcgaatg tccggagata agctatgatg gaaattcgcc
    58261 aattatcatt atcagggacg acgaagatac tacttatagt ccgccagtaa taattaaaga
    58321 atccgaagat accgttggaa taaaaatcct tgacgtgctt tcaaaaaaca gaaaaatcgc
    58381 ccgcctttac aaagatacag tgatggtgaa aaacaatcct tatcgcattg tgtatttcat
    58441 tgatacaact gaggttcacg gaataaataa aaattattcc ggagacaact tttgcgttat
    58501 cactcaggaa aaggatttac cgatatataa tcgttatcag gtgtttcatg tatcctattt
    58561 cagacacgga aactattttg cagatgtatc tttactgaac agagtccgga aaaagataat
    58621 cgctgttgat tgcgagctga aaggcgacgg ggcagacaat tggaaagttc accgtataaa
    58681 aattaaaaaa agaaatattt ataaaattaa aagatgaaat cctgaaacga acaatgctac
    58741 actctcattc tatttttaat catcgccaaa agattctgaa aaaatttatt cctctgtttt
    58801 tcagccatac cactcagctg cgtcgattta cggatgaggc cggtgagcca gtcgcgtgtg
    58861 gcagcacaga aatcttcgtc ggcagtttca agtgtattga atgttgagtg gcgcagaaaa
    58921 acttttttat ttttcccaat cgaagtataa atacaattgt tgccgatttc gatatcgcag
    58981 cgataaaatg tataattttt gtcgcccgca tgatcttttc gcttggtccc gggctcagtc
    59041 atgctgcgga tttcggacat tagtgataat aaatctgcat ataatttctg cgttgtttcg
    59101 ctgtctttaa aaaagccact ttccgaataa aaatcgattt gtttgagaat gctgttcaat
    59161 gtctgttcac tccatatctc ggttgaaggc actatgcgat acgattcaag gatttcattg
    59221 ccgatgctca tcaattcttc aggtgctact tcatcctcga aaaagctatt ctgatattcc
    59281 ggaacattca tcaccgagcg catccagtaa tagcatttaa accaggccag ctgcggaaaa
    59341 gcgaataaga taaaaaaagg aatatcggcg gccacataca tgatttcacg ttctttggca
    59401 ttttttaaaa tcatcatttc atcgcggata cgacaaagat attgttgaaa aagctcaatg
    59461 gaatcgaccg gccattgata ttgaaaataa gcagaaccac gatgcgactc acaaaagcca
    59521 tcgaacgaaa tatgaaaatg gttacaaagt ttcaccattt cactgagcga aaattcagtt
    59581 tctccgcgca atcggcgata gacactatcg ttactgagcg acaaagctgt tccaacctca
    59641 gcaacaagtt gcaccccgga aggcacaata ttccgaagca atctgataaa attttcctgc
    59701 actgaaaact gatcactcat acgatacaat atttttcaca tgcaaatatg cattttaaag
    59761 atgagaaata catatgaaaa tcaaaaaaaa caaaatatcc taaaaaacat taaaaaaacg
    59821 ccaccaactg aaaaatcaca aatagtagcc atatacatat tttttacaca tattcagctg
    59881 atatttatag tttaaataaa atatttaaaa caatttgctc tttcatttgt ttcaagtcta
    59941 aaattaaaga ataattgctt taaaacaaaa atcataagat tttatgccga ttgaaaacaa
    60001 aacatattta ccaatacact atacgttaaa aatttcccaa gcaagataat ttaaaaatca
    60061 aatgcaacta tataatgtat taagttgtat tattgttgat taaataaatt aaaccccatg
    60121 agaaacattt acgcattatt aatttttgtt gtttttcttg ccttcagttc ccatactgaa
    60181 gctcagacgc tgaaagaaaa cacccaacaa ttacagaccg aacagacctc aaatgtaatt
    60241 tatccggtac gtgcctgggt aacaaaacct ctgcgtgaac tgacacaaga gaatccctgg
    60301 cgcgagcccg atttcacaac aatcccatgg gtatcacccg accggaaaaa caggccgcgt
    60361 cagactttta tttattcagc tgccgatggt cccgaatatg gcaatgactc ttcaatcatt
    60421 caaacaaaaa tgggaacccg cgatgcgaca caatcgacaa aagcacttat taaaaactgg
    60481 gcgggaattc aaagttcatc atatccacca gatcccacag gagcggtagg acccaaccat
    60541 tatatccaaa gcgtcaatgc aactactgtt cgcatttttg ataaaaacgg aaacacattg
    60601 gaaagctttc tcatggggac tattttcgga gaaacatcca atgccggtga cccgattatc
    60661 atgtacgacc gatttgccga tcgctggttt gtaagccagt ttggagacaa taatgaaata
    60721 tttattgcca tttcacaaac agcagatccg accggatcct actatgctta cgaatatgtt
    60781 tcaccacagt ttcctgatta cctgaaattt tccatatggc acgatggcta ttacatgaca
    60841 tcgaaccaaa gcactgataa agtctatgtt tttgagcgcg acttaatgct aaccggaaac
    60901 tccggagcca gagccattta tcagacattt accaccggtt cagtatcggc cttttttgtt
    60961 ccgctgccag ccgatgcagc cgacaatcat acattaccag ctgccggcac accactgcct
    61021 ttctttgctt attacgataa cgcctggggt gggggtaccg atggaataaa aatctggact
    61081 gccaccacca attggtcagc gggcactcta accatttcga ccacacctac acaggtaaac
    61141 acaagtgcat ttgatgcctc atacgattcc aactgggacg atgttcccca accaaacggc
    61201 gttgaacttg acggaatcgg gggcgtactg atgtaccggg ctccgtggcg ttcatggagt
    61261 ggctataaca atgttatttt aacatgggga gtaaaaattt cagaatcacc aaggcagcga
    61321 gccatcatgt ggtgtgaact tcgccagatt tcaggcacat ggagtgttta tcagcagggc
    61381 atctatgcac ccgacacgca tacccgctgg atgagcagcg ccaccatgga tgataacggc
    61441 agtattggat tatgctatgc caaatcttcc accaccattt atccgggttt atattacacc
    61501 ggacgtcttg caagcgatcc gctaggcacc atgacgttaa ccgaaaccca ggttgtggcc
    61561 ggaaccggtt cgcaatccac atacaaccgg ttcggagatt attcacagac atcgcttgat
    61621 ccggatggag ttacattctg gcataccgga gaatatgtaa ccacgacctc cgggcaggaa
    61681 acccgggtat attctttcca gattcctgcc ggtccgctcg caccagtagc caatttcaca
    61741 gctgatgcaa ccgcgccgtt ttgttcaggg acggttcaat ttaccgacat gtcgtccaac
    61801 gctccgacat cctggttatg gaatttcggt gacggacaga catcgacaca acaaaacccc
    61861 tctcacactt atacagcaag tggcacctat actgttacac ttacatccac caatacattg
    61921 ggcaatgatt cggaagtaaa gaccagttac attaccattg acattccggc tacagcatcg
    61981 gccacaaatg cagaacgctg tggaacaggt acagtaacac tgtctgcatc gggaacaaac
    62041 accctgaact ggtatgatgc agcaagcggc ggaaatttac tcggcactgg aaccagtttt
    62101 accactccca gtatcagcac aacaacaact tattacgttg agaacaatat tccttcagct
    62161 cctgcaaatg ttggacccgc agtcggaggt gcttcacgca acattgcagc cattttgtat
    62221 ttcgacgtgt accaaccgat gactttaatt tcagtacaag ccagagcaca aacaacaggc
    62281 gtaaaaacaa tcacattgct cgataatacc ggagccaccg tttacacggc caatgtaacc
    62341 gtaaataact cagtaacgac cattacattg aattgggtta ttcagccagg tagcggatat
    62401 cagttactga ctctggaaaa ttcagcatta taccgtataa gttcaggtgt atcctatcct
    62461 tacactgcac cagggttgtg tagtattacc ggatgcgata ttggcagtgc ttattattat
    62521 tcatggttta actggaatgt ccagggatta ggatgcggaa gcacccgtgt tccggctgtt
    62581 gcaacaatca atccaaccgt aactccctca gtagccgtta caccttcagc aacaaatatc
    62641 tgctcgggta caggtgttac atttaccgca acgccaagca atggcggaac accttcgtac
    62701 caatggaagg tgaatggcaa caatgttggc agcaacagcc caaccttcac atccagtgca
    62761 ttatctgacg gagatgtaat tacatgtacc atgacttcaa ctgaatcatg tgcatcacct
    62821 gctacagcaa cttcgtccgg tataaccatg actgtaaatc ctgttgttac tcctgctgtt
    62881 tcgattgctg cctctgcttc aaccatctgc gatggaaaca gtgttacttt cacagcaaca
    62941 ccaagcaatg gcggaacgcc tacttatcag tggaaactca atggaaacaa tgtgggaaca
    63001 ggtggaacaa cctataccaa ttcaggactc aatgacggcg atatcgtatc ctgtgtcatg
    63061 acatcaacag aagtatgtgc taccacaggc actgccaatt ccaatccggt tacaatggtt
    63121 gtcaacccca atgtaatccc gtctgtagct gtttcagaat ctcaaaacaa tatctgtgat
    63181 ggcaccaatg ttacatttac cgcttcgcca agcaatggcg gtacaccggc ttatcagtgg
    63241 tatctcaaca gcaatcctgt cggatcaaac agcgcaacct acagcaatgc agctctcaat
    63301 gacggcgacg aaatcacttg tgttatgaca tcgacagcag catgcgcttc cccttcgtct
    63361 gtttcttcca cccctgtaac catgacagtg aattccgctg taacaccatc tgtttcagta
    63421 aatgcatctt ctacaagcat ctgcgatgga accaatgtta catttacagc ttcgcctgtt
    63481 aacggcggta ctccgactta tcagtggtta ctcaacggaa acaatgttgg ctctggcggc
    63541 acaacctatt caaatgcatc cttaaatgat ggagatgtta ttacttgtat catgacatcc
    63601 actgaaacct gtgctacacc aacatcagta acttcattac ctgtttccat gagtgtaagc
    63661 gctccggtaa ctccttctgt agctgtttct gcttcggaaa cgaatatctg ctcaggcacc
    63721 agtgttactt tcaacgcaac accaacaaac ggcggaacac caacctatca atggcaagtc
    63781 aatggcagta atgtcggaac cggagccagt tataccaatg cttcactgaa cgatggagat
    63841 gaaatcacct gcatcatgac ttcgacagcc gcctgtgcaa cccctgaaac agttacatcg
    63901 gcgccggtaa acatgaccgt caacacaacg cttacacctt cggttatggt atctgcatcg
    63961 gcaacaacca tttgttccgg ttccagtgta accttctctg caacgcccac caatggcgga
    64021 acaccgtcct accagtggta tctgaacgga tcgccaacag gaataaacag tgcaacctat
    64081 tccaacaacg cattaaatga tggagacgtc attacctgtg aaatgacctc ttcagaaatc
    64141 tgtgcctctc ctgtccttgt tgtttcatca ccagtaacta taactgtcag ctcaagcgtt
    64201 actccatcgg tttcagtcaa cgcttcatca acgaatatct gctcagggac tgccgttact
    64261 tttacggcaa cgcccaccaa tggcggaaca ccggcctacc aatggtacct caacggatcg
    64321 ccaacaggaa taaacagtgc aacttacaac aatgcagcgc tcaataacgg cgacgaaatc
    64381 atctgcgtga tgacatcgac agaatcatgc gcctcgccaa cttttgctac ttcagcacca
    64441 gttacaatga ccgtgagcag cacattaacc ccggccgttt cggttgctgc atcagccacc
    64501 agcatttgtt cagggaacaa tgttactttt accgcaaatc ccgtgaatgg cggaacacca
    64561 gcatatcagt ggtatctgaa cggatcgccg gtaggaggaa acagtgcaac ttacaatgat
    64621 gcagcactca atgatggcga cgaaatcacc tgtgtcatga cttcgacaga attatgcgcc
    64681 tcgccggcta cagccacttc agcaccggtt ataataacgg taaacagcac actgacttct
    64741 gccgtttcgg ttgctgcatc cgccaccagc atctgttcag gaaccgatgt tgcatttacc
    64801 gcaaatcctg ttaatggcgg aacaccgaca tatcagtggt acctcaacgg atcgccggta
    64861 ggaggaaaca gtgcaactta caataattca ggactcaata acggcgacga aatcacctgt
    64921 gttatgactt cgacagaaac atgtgcttca ccaactacgg caacatctgc tccggtcacc
    64981 atgattgtca atacaagtgt aacaccctct gtcgcaatca atgcatcagc aaacaatatc
    65041 tgctccggaa ccagtgttac atttactgca acacccacca atggcggaac accggtttat
    65101 cagtggtatc tcaacagcaa tcctgtcgga tcaagcagcg caacctacag caatgcatcg
    65161 cttgctgatg gcgatgttgt cagttgcatg ataacttcat ccgaaacatg tgcaactcct
    65221 tccacagcaa cctcagcacc tgtagtcatg acggtaaatg ccaatgtaac accatcggtt
    65281 tccataaccc cttcagcaac caatatctgc tcgggcagcg aaatcacatt tacagcaaat
    65341 ccggtaaatg gaggaactcc ttcatatcaa tggctactca acggatccgt tacaggaacg
    65401 aatagtccag tgtattccag ttcgacatta aacaacggcg acattgttac ctgcaccatg
    65461 acatcgacag aaacgtgtgc agacccggtt acagcagttt cctctcaggt agcaatcacg
    65521 gtaagcacat cggtaacgcc atcggttgcc atcaatgcct cggcaaccag tatttgtgaa
    65581 ggcacctcgg tactgtttac ggcaacaccg gttaatggcg gaacgccagc atatcaatgg
    65641 aaaataaacg gcaacaacgt tggcggcaat agtgcaacat tcaatagcac atcactcaac
    65701 aacggcgatg taattacctg cgaaatgact tcaacggaaa cctgcgccaa tcctgcgtca
    65761 gttacatcgg gaagcatcac catgaccgtt gtgccatcag taacaccgct tgtttctatt
    65821 gctgcatcaa ccaatgatat ctgttatgga acaccggtta catttacagc aactccaacc
    65881 aatggcggaa cgccaacgta tcaatggata ttgaatggag ccaatgttgg cagcaacagc
    65941 ccgaattaca gcagctcgat gttgcaaaac ggcgatatta tttcctgcat catgatatca
    66001 aatgtcagtt gtgcaacagc cactacagtt aattcaaacg acctgacgat gattgtgaat
    66061 ccatctgttt caccttcggt ttcgattgtt tcatcagaga atggagtttg tgaaggaacg
    66121 cccattacct ttaccgctgc gccggtcaat gggggcactc catcatatca gtggctggtc
    66181 aacggtgcca atgttggcag caacatgagt acttttacat caacaacact aattgacggc
    66241 gatgttgtta gctgtgtgat ttcttcaaca gcaccatgtg tttcgcccgt gatggccact
    66301 tcaaacacga ttgttatgaa tctttatccc aatctgacac cgtctgttga cattgcatca
    66361 caaccagccg gcaccatctg tccgggcaca accaaagaat tcacggcagt agccaccaat
    66421 ggtggaacag caccgacttt tgaatggttt gttgacggcg tttccgttgg aaccggatca
    66481 gttctgaatg gagtatattc caatggtcag gttatttcat gtaccatgac atcgacacaa
    66541 ccctgtgtaa tgccagccat tgttgatgca aacccaatca cagtaagcac attcaatgtt
    66601 actccggtta cgataagtga taatggcggg acactaactt catctgccgc atacggaaat
    66661 caatggtatg aagtcagcag cggtgttgtt gttggtgcca tcggacaaac atttgctcca
    66721 ctctacgaag gtgtttatta tgtcgttgta accgatgcca atggttgcac aacaacatca
    66781 aaccaaatag aattcatcct agaaagcatt gatgaaaacg gagctgcaac cgttacattc
    66841 tttccaaatc caaccagtgg aaatttagct attacattca acacggcctt caacaacggt
    66901 acgctgaaaa tagaaaacgc cattggagaa ctggtctttg aaaagaatat tacacagagc
    66961 gccggaagcg tgtttacgat tgatttcagc gcttatgcac ctggttctta tttcatcacc
    67021 gtaaaagatt ctgaaaacga aataaaaaaa caggtgattt acgaaaaata aaaattgcat
    67081 ttataccaaa aaggagtctc tttccgggac tcctttttta tttttattaa ctctttatta
    67141 acgccacgct tttcactatt tattcaatct aaaaacaaat tgttgtattt ttacaattca
    67201 ggttgtaaat tgcggggtta aaaagtcaaa caaaaaaacc accatatgaa aaaaattttc
    67261 tttcccttca tgctcataac gctggttctt gcttttgcat cgtgccagaa agacaaagaa
    67321 ggagacaaca ccgatttgaa tttcgggtat gtaaaaggaa atgtttacac tcccagcgga
    67381 gttcctgttc ctgcagccag catatttatt gatgttgaag gcgaaattta ttttgcccgc
    67441 accgataaaa acggatattt ctatcttcgc gccccagagg gcgaacgtgt gttgaatctg
    67501 caaggcggtg atggaagcat tttccgctca caatacaatg ttgaaataaa agctgatgcc
    67561 gttactgcat ttcccgacaa tacgctaaaa atttcacagg cagaagaact ggcctatata
    67621 ccaggagctt ttgaccatat tgaaacaata ctcatcgact cactcggata tactgcagac
    67681 atgctttcgg tagccgatct tgacaatctg acattgcttg aatcgtatgg cgccatcttt
    67741 ctcaactgcg gaaaaaccgg aaacatggat agtgtcaagt atgaaaatct tgaacaatat
    67801 gttatgaatg gtggaaacat atatgcatcc gactgggccg tagaatatct caccggcgat
    67861 ggcaactttc ttgcaaattc ctcacaacca tttgtcatgc atgaacacaa actcactcat
    67921 gcttccaatc cgaaatcagg ctgcctgagt cccgaagtgg gtggattcat tgacgacgca
    67981 accctttgta ccgacaaaga cggcccctcc accatgctat atcaggccga cattgtcgat
    68041 acccttattc aggccttttt gggtaaaacc aaaatcgata ttgaatatga tctgggcgca
    68101 tgggaagtta ttaaagacct taccattcct tgggaagtac ttattcagga ccctgtaaca
    68161 tatgggccac tggctgttcg catgaacttt ccaacacagg cagccacacg cagtcaactc
    68221 gaccagggct gggtaacaat ttgccacttc cctccgggaa atccctccaa tatgcaaacc
    68281 attaccattt caatcaatgc attgccagcc catcttgccc acggtgatta tataggcagt
    68341 tgtgtcggcg gatcgggaac cattttcttc actacattcc acaatcacgt acaaggcaat
    68401 atcggaccgg atacctatca gatgttgcaa tactttatca tgaatttgta gtccttacaa
    68461 atatttttaa aaggcttcct tttggaggcc ttttttattg ctgatctatt agtacggttt
    68521 tgaaaagctt atgcccgctg tcatttagag atcaccagca aaacgtgttt tgcaacagaa
    68581 atacgaaatg acaatgcgac aaacataata ttccatcgtc ctatggcgac gaaggagtca
    68641 gaggatatgg aattccacca ttaggcggac aggctgttat gtttgctcca aaaccgacga
    68701 ttccatattc tgcgattctc tccgggagtt tatcacgccg tggcgtgaat ggaatatcgg
    68761 ttttgctgct tctttggttt gagaaatatg ttgtgcttag cctattcaaa caaagcactc
    68821 atatcattca gcgaaaagct aaacaacgca gtctcgctca cctgcacctg tttgccgctg
    68881 ctcatctgct ttagagtaac aataccggca gcttgttcat cgggaccctg aatgagcgtg
    68941 tatggaaatg catatgcatc ggcataggaa aattgttttt tcattttggc tgcttcggga
    69001 tacaccacgc atgcaatgcc gttttcgcgg agtctcttcg ccattttcat tagatctgcc
    69061 gcactgtctt cgctgaaatt aatcagcaga atatcagcgg tgcgactgat gttttcgggg
    69121 aacagattca actccatcat cacgtcataa atgcggtcgg cgccaaacga aacgcctacg
    69181 cccgaaacac ccggcaagcc gaaaatgcct gtcagatcat catatcgacc acctccgcaa
    69241 atgctgccca tggctacatc gcaggccgac acttcgaaaa tggcaccggt ataatagctg
    69301 agtccgcgcg ctagactgac atcgaatttc acctgtgacc tgagttcaag cgcatcgata
    69361 aaaccaagca gttcctccat ttcagcaatc cccttcatgc cggcttccga aacaaggtat
    69421 ttcttcaact gcgtcaacat ggcttcgttg ctgctgaccg gctgcatgat gtccatgagc
    69481 ttctgcggaa catcagtcgg aaaaccgcgc tcaatcagct ctttgccgac accttcccat
    69541 ccggttttat ccagcttgtc gagcgaaacc gtgaattcaa caaagcgatc gctcacaccc
    69601 agtgcatcgg caatgccctg cagaattttg cggttgttga ttttcaacat tacgtttacg
    69661 cctaatttct gaaacgccgt atcaagaatg tccatcagac ctgcttcctc catctggctg
    69721 cgagtgccga ttacatccac atcgcactga tagaattcgc ggtaacggcc tttctgtggg
    69781 cgatcggcgc gccacacggg ctgcatctga tagcggcgga acggaaaaga aatttcgttc
    69841 tgattcatca ccacaaaacg tgcaaacggc acagtgagat cgtagcgcaa acctttttcg
    69901 gaaatatgtt tgatgacttc gctgcttgta tgttcttttt cggccggaac cgaattcatg
    69961 aaatcgcctg aattgagtac gcggaaaatg aggcggtcgc cttcttcgcc gtatttgccc
    70021 atcagcgtgg aaagctgctc catcgatggt gtttcgatgg gctgaaatcc atgcttttcg
    70081 aacaccgaac gcagaatgga gaaaatataa ttcctgcgcg ccatctgcgc gggagaaaaa
    70141 tcgcgtgtgc ctttcggaac ggacagaaca gtctttgcca ttgaaaagtt ttttgcgaaa
    70201 ttagtgaaaa taagggaaac ccaagtaggt aagggcgagg gcgaggcatc gaaagcgtga
    70261 aataactgtg aagcacaatt gatggaggat ggtcacgcgt caggagacgc gcgcccatgt
    70321 cgggaggtat ttattcatta tgtattacga aaacatgtcc attccaggga taacaacttc
    70381 ctctaccgac tgtaaaacct ctaatggaaa atataaccga gaaaagaata ataacagaaa
    70441 acataaccaa caatattctt gagattttct ttctctttga aaaaataata aacattacca
    70501 acaaaaatat tgaccaggca atttgaaaat aaaatggaat taagtctgca aggcaccctc
    70561 catacgtaat tttcccagcc atcattaaga taattaccag tacaaaaaaa cctatcgctg
    70621 acaatatgat tgtattaaat actcttgtca tcactgatat aattaaattt tccgaactca
    70681 tttcctcaca aaaatcctct tcagccgcgg ctctgaaaaa gaaatgtata tgacaaacac
    70741 catcagcagc agtgtgttga aagccagtcg ctgtgcaaca ttgtcggcga agagatgttg
    70801 tgtggtatat accgaaacaa aatacaatcc tgcactaatc atcatgtaag ataatatgcg
    70861 ctttaaatcg taattgacgc gatagtattt ttgtccgagg aaataggaaa tgaccatcat
    70921 cactgcatag caggcaaagt tggcccaggc gctgcccatg tagccgataa ccggaatcag
    70981 tgcgatgttc agaccaactg aaatggcagt tccaatcaaa ctaatgtaag caccaaaacg
    71041 tgtttgtccg gtgagtttgt accaaatgct aaggttataa aaaatgccca gaaaaacatg
    71101 gctgtagagc agaatcggaa caattttcaa tccgctgtaa tactcttcgc cgacaaaata
    71161 ttttacaatg tccatgtaca gaatgatggc cagaaacata gctgtcatga tgatgacaaa
    71221 atatttcatc acatcggcat aggtttgttt ggcatccgca tctttttgcc gtgcaaagaa
    71281 aaagggctcg gctgcatagc ggaatgcctg aacgaaaatg gacatcacaa tagcgatttt
    71341 aaaacacatt ccatagattc caagctggta catggccaca tcttcgggca aaagatattt
    71401 caataaaata cggtcaacgg tttcgttggc aattccggcc agcccaagaa taagcaatgg
    71461 agcggcgtac gacaacatcc gtttcagcag tgaaaaagaa atgccaaatt tgattttgaa
    71521 tatatcagga ataagtaaaa taaaaacaac aaccgaagca acaaggttgg aaacaaaaat
    71581 atgtcccaca accggctctc catcgcagat ccacgacaac atctctggtc cccacgaata
    71641 atttttgtac agataaggca ccagtaaaag caaaatcaga ttcagcccaa tgtttacaaa
    71701 aatgccgatc agtttaatga tggcaaagcg catggctttg ttttcggcgc gcagccttgc
    71761 aaacggaatg cttgtaaaag catcgagacc gacaatcagc gcgaagtatt gaatgtattc
    71821 aggatggtcg ccatagcgaa gcattgcggc aagaggctgc gcaaaaatca gcgcagctgc
    71881 gataaacaat gcactcgttg tgaaaagcga agtaattgtt gtagaataga cacgattttt
    71941 attctcttct ttctgattga aattgaaaaa ggccgtctcc attccatacg tgagaatgac
    72001 gatgaagaat gcgacatacg catacatttc gacatatacg gccgattctt ccggaagaaa
    72061 aacgcggctg tacatgggca ccagaaaata attaagcagg cggcccacaa tgctgctgat
    72121 gccatatacg gctgtttgtc ctgccaaact acggagattt cccacaatga aatattatgc
    72181 ggtaaaatta cattatttcg cggtacgaag ccaatccatt tttttcttcc gcgacgggtg
    72241 cgaatgttcg cgaaattttt atccgacctg cagcgcaggt caaacaataa tagcccggcg
    72301 aagccgggtt aaaaatcacg gtatattgga ccctggcagg gtccaattat tgcctgctat
    72361 aatacatccg gcttcgccgg acgctattat tgttcgacat cctccggacg tcgggaaaat
    72421 ttttcacaaa aacatgtagt accaaagcta cacatcaagt gccttacccc agataagtct
    72481 tgagcagttt gctttttgaa ttgtgcttca ggcgacgcaa tgctttttcc ttgatctgac
    72541 gcacgcgttc acgggtgaga tcgaaatgca cgccgatttc ttcgagagtc cagctgtgat
    72601 taacaccaat gccaaaatac agattgatga tatcgcgttc tttttcactc agtgtcgcca
    72661 gcgaacgttc aatttcgcgt gaaagacttt cgctaataag attctgatcg gcacgtggag
    72721 catcgtaatt gaccatgaca tcaagcatgc tggtagtatc atcgctgaca atgggcgcat
    72781 cgaccgaaac atgacgtccg gacaaacgca tggtttccat cactttgtct tcaggaagat
    72841 caagactggt tgcaatttct tctgcagaag gcgggcgctc atagcgctgt tcaagtctcg
    72901 aagcttcctt cgaaatttta ttgagcgatc ccacctgatt gagaggcaga cgtacgatac
    72961 gtgactgttc ggccaaggcc tgaaggatgg actgacggat ccaccaaacg gcgtaggaaa
    73021 taaatttgaa tccgcgggtt tcatcgaaac gctgagcagc tttgataaga ccaagattac
    73081 cttcatttat caaatcaggc agactaagtc cctgattctg atattgtttg gccaccgaaa
    73141 cgacaaagcg caggttggcg cgggttaatt tctcaagagc aatctgatca ccttgtttga
    73201 tacgctgagc cagctgaact tcttcttcgg ccgttatcaa ttcttctctc cctatttcct
    73261 gcaggtattt atcaagtgat gcggtttcac gattggtaat ggattttgat attttgagtt
    73321 gtctcatagt ctgattttgt taaaatgctg attagcagcg ttttgcgcaa gttttacaaa
    73381 tagtgtacaa aggtacgctt ttttaagcca aaaaccaagc tattttagct gtatttaaca
    73441 gcttataatc caaaaccata gtatgctttc attccattgg gataaacgcc ttcaatcagg
    73501 acgccaccgc tttttcctga caatgcattt tctatatctt ccacggaata aataatggtt
    73561 tgatcgattt tggtaacgat aaaactttct tttattccgg catttttcaa tgcgccttcg
    73621 ttcaattcag aaatctgcat accatttata agccgcaaac gactcattgt ttgctgatct
    73681 ggttctgaaa aggttgcccc cataaaagta gataaagcag gatcttcttt ttttagcaac
    73741 actttttcac ctctacggtt taataaagta accggtttgg aaatggtttt atttcccctt
    73801 tcaatttcca acaacagttt atcccccgga tgcttttcag cgatcagctc caggagccgg
    73861 cttgagctat tcacctcttc accgccgact gacaggatga catcgcccgc ttcgattccg
    73921 gcctcaatag ccgcccctcc atcactcacc gccgaaacat atacaccttt catactcgat
    73981 aagccatact gtttggcagt agcagcagta atatcaacaa tatcagcacc cagataagct
    74041 ctttgcacca taccgtattc cttcagatca gaaaccactt tactggcaat atttgaaggg
    74101 atggcaaatg aatatcctga ataagagccc gtattgcttg ctatggccgc attgatgcca
    74161 atcagctctc ctttggtgtt tacaagtgca cccccgctgt ttccgggatt gacagcagca
    74221 tcggtctgaa taaaagattc aaccgaacta cctccgccaa ggatgtttat gtttctggct
    74281 ttggcgctca caatcccggc tgtcacggta tttgtcagat tgaatggatt tccaacagcc
    74341 agcacccatt caccgatttt cacctggtcg gaattaccga aaatcagaaa cgggagattt
    74401 ttctcatcaa ttttgatcaa tgccagatca gtcgatggat caagcccaac aacagtggcc
    74461 acaaattctc ttttatcatt cagcgttact gatatctgtt cagcatcctg aacaacatga
    74521 ttgttggtta caatatatcc atcatcgctt acgataacac cacttccggt tgcattaatc
    74581 ggatattgct gcgaaaaggg ctgtacatta aaaaaatcat aaaaaggtgc aaaaaaatca
    74641 tcataatacg catttttacg cgtgaaagaa gtcttgatat gaaccactgc cggcaccgat
    74701 ttatcagccg ccattgtaaa atcaacaggg tcaccgggca caggtttcat catcggttca
    74761 gccagcatta tttctgtcga aaaaatcaaa aaaaacacac cggccatcat ccggaacact
    74821 atttctctct ttttcataat caatctgttt attttctctt tccaaatttt gttcctttca
    74881 gaatcatcac agataacgcc gataagtcct ttttattgta ttcatgggct ttccagacta
    74941 tccaaaaaat aatcaacttc ggaaatattt atgtactttt gcaatgcaaa gtgacaattt
    75001 gtcactgcaa atattattca ttcaatgaca ggggacagca gcaaacatca tcgaaaacat
    75061 ctgaaccgct cattcatcac cggaatgatt atgggagcag cattgttgct gggcgcttac
    75121 ttcacatatc tgcttattac caacgacgag caggaaagca tcagcagaaa aacagcgacc
    75181 gtcaaaaacg attcaattcc cccgctctac aatctgaccg gatttccccg aatcctgttc
    75241 gacagctcat tacaaaatat cgacagttca tattatatca acaaatggaa tcagctgatt
    75301 gaaatgaacg gataccgcga aatggactcc ctctttatcg attctgtcat ccgcaaaatg
    75361 tatttcgact ccatccgggc ggtttccgat gtatctgctg ataccatcat tctgcaaaac
    75421 gaccgcttga tagcaacaaa aaacatcaat gtcagaattc aaaaaaaact gctgctgaat
    75481 gaagatacaa caggttcaga ctacatatat tccgacgcga aatttatcat tgaattctgg
    75541 gaatcaccca ttaaattcag aggatttaaa agaacaacaa attatgtggt tttatttggc
    75601 ctgaatccgg aaaaaacgac aacatttcta caaatcgaca acatcctgta tatccgcgaa
    75661 aagcaaatct ggtacgctat tccttgttct cccgaatttt cgggactggc acctgttacg
    75721 tcacctcaac tgctttcaaa aatcaatgct gccaacaaat aaaaacctgc gtatgaacac
    75781 agcatataaa tttcacggcg ccggcaatga ttttatcctg atcgattgtt ttgaaaaaaa
    75841 agagttgttt tctgaaaaag agattgaagc gttatgtaac agacacaccg gaatcggagc
    75901 cgacgggctc attctgatag aaaaaacaaa caaagcttct ttcaaaatga tttatttcaa
    75961 ctccgacggc aaagaagctg agatgtgtgg aaacggcgca cgatgcgctt ttgcatttgc
    76021 tttctcgaaa aaatactgca ccatcaatga ttctttcgaa gccagtgacg gtttgcatat
    76081 gggaagcatt atccaaaaca atactcacga ctggattgtt cgtatcacat tgaatgtaaa
    76141 cattgaaccc cgtgaaatag aagatggctc tttctttgcc aataccggcg ttccgcacaa
    76201 tgtaagaata acaaaaaacg tcagcggcat tgatgtaaaa accgaaggat taaaactgcg
    76261 atacaacaaa aaactattcc ccgacggagc caatatcaac tttgttgaaa tcaaagacaa
    76321 cgaattatac atccgcacat acgaacgcgg cgtggaagaa gaaacactgg cctgtggcac
    76381 cggaataacg gcctccgctt tggtggcaaa tgcatttttc gggcttaagt ttcctattac
    76441 aattcatgca atagggggac ttcttcgcgt tgaaaaacaa aacggatggc tatggctcga
    76501 aggcccagcc agtcatgtat tcacatcaaa ttatttctga tttttatgct gaatggatcc
    76561 gttgctttct tgcgaacacc cgacatatca gatgttcaat tcatgcttga cattgaaaac
    76621 aacgagcgtt actggcatct gagcggcgtc acttcgcctt acagcaaaaa agatattgaa
    76681 caatttatta atgaatcatc atcgaatctc aaaacagact gtcaactgcg ccttgtcatc
    76741 attgatattt caacaaaaaa gcgggccgga ctactcgatg tatttgaata tgagccattg
    76801 cacaaacgcg ccggaatcgg catattcctg ttggaagaat tccgtggaca cgggcttgca
    76861 agcgatgccc tgcgtgttct gatacaatat cttttcaaaa tcatgaaatt gcatcaattg
    76921 tggtgcaaca ttcttactga aaatacagaa agcatgaaat tgtttaatgg cgcaggtttt
    76981 aatcaaacag ccattaaaaa ggactggata ttccataatg gcaaatttca cgatgaagcc
    77041 atatttcaat tgataaatcc gaatcacagg gtatgaaaaa aaaactgatt ttcattgttc
    77101 ttgcattatt gatggttgtt ggtattaccg gggcctggta tgtttataca aaagctttta
    77161 gtgacaatat aaacctgaaa gcaaaaacca aaaaaataat ttttctacca accggcggaa
    77221 atattcaaaa cctatacgac accttaaaaa aacacgaaat cctgaaaagt ttttcttctt
    77281 ttaaaatgct ggcagaatac aaaaagctcg ataaaaaata taagcccggg cgttatcaga
    77341 ttgccgcagg catgagcaat aatgcactgg tgaacatgtt tgcgagcgga cggcaaacac
    77401 ctgttaaaat cactttcaac aatatccgaa cacgcaaaga tttggctgag cgaataggaa
    77461 cacagctgga atgtggtgaa gaggcattgc tgaaaatatt aaacgatgag aaaacagctg
    77521 aaaaatacgg attaaataaa gacaattttc ttgtattgtt catccccaac acctacgaat
    77581 tttactggaa cacgagtgct gatcagttta tcgaccgcat gcactcggaa tggaaaaaat
    77641 tctggacagc tgaacgcctg caaaaagccg aaaacattgg gctcactcct gccgaagttt
    77701 cagtgctggc ttcgattgta aaaggcgaaa caaacaaaag cgacgagatg gataaaattg
    77761 ccggagtgta tatcaatcga ctgaaagatg gatggaagtt gcaggccgat ccgactgttg
    77821 tttttggcat cggcgatttc accattaccc gtgtgttgaa aaaacatctg gaatacgatt
    77881 cgccttacaa cacttacatg tacgaaggac tgccgccggg acccatcaac ttacccgatg
    77941 caacagtgat tgacaaagtg ttgaattaca cacaccacaa ctacatgtat ttttgtgcca
    78001 gagaagactt ttcgggatat cacaattttg ctgttacatc aagccagcat agtgccaatg
    78061 cgcgcaaata tcagcaggca ttgaatgcat gggaaaagaa gaaaaaggac tcgggaaaat
    78121 aattcgaaat aatttatcag agctgtcctg ataacttatt ctgcgccatt tcgcaatgat
    78181 tcaagtgctt tttcggcagc attttgctcg gcacctttta tgctatagtc aataccccga
    78241 ctccgggctt cgccatcaat acgggcttcg cacacatact gtcgtttacg tcctgtctga
    78301 atttcatcga caatactgaa ttccagattc agtcgttctt tttgacaaaa ttcaatcagt
    78361 ttgcttttaa aattggtctc ggtactgata atagcatccg catctattct tcgtccaaaa
    78421 ataaaactga ccaataaatt ctgcgcaaaa ccatagccct tatcgagata aacagcgcca
    78481 aaaaaagctt ccagtgcatt tcccgtataa gcattattgg cgggtacacg gctcatcgta
    78541 accaaacgtc caacaccaag tttttcggcg attttattaa gtgaattgcg actcaccaca
    78601 cgcgaacgca tttcggtcaa aacaccctcg tcggcaaatg gaaatttctt aaataaataa
    78661 tcagcaacaa taacactaat cacagcatcg cccagatatt cgagccgttc attggaaatt
    78721 tcatgaccat ttcgttccac atgcgctgat ttatgcataa aagcaagttc gtataaaaaa
    78781 atatttccgg gccggaaccc gaaaatattt tttatgcttt ttgaaagttc gtcagcgtgt
    78841 ttctttctga aaattcccac tattcaaatt ttttgaaagc aaggcaaaca ttgtgcccac
    78901 caaagccaaa agtattactc aaagcatagg tgatattgcg ttcttttacc ttgtcatagg
    78961 tgaaatcgag agaagaatca atttccggat catcggtaaa atgattgatg gttgccggaa
    79021 taattccgtt tttcaaagaa agaacaacgg caatagcttc gatggcaccg gcagcgccca
    79081 acaaatggcc gtgcattgat ttggtggagt tgattgaaat tttcggtgca tgttcaccaa
    79141 aaagagagac gattgctttt ggttcagcaa tatcgcctac cggagtcgaa gttccatgcg
    79201 tgttgatatg atcaacatcg ttgggagtga ttcctgcatc tttaatggcc gtttgcatag
    79261 ccgtgcgggc accttctcct tcgggatgcg gagcagtaag atgataagca tcggcagaaa
    79321 gaccaaatcc cactacttca gcatatattt ttgcaccacg tttcaccgca tgctcatact
    79381 cttcaaggac cagacctgca ccgccttcac ccagaacaaa accatcacga tctttatcga
    79441 aaggacgtga agccgtttta ggatcatcgt tacgcgtaga gattgcatgc atagcattga
    79501 aaccgccaat accgccctga ttaatggcgg cttcggagcc accggcaacc atggcatcag
    79561 catagccgtg ccgtacaagc ataatggcat ccgcaatggc attggccgaa gatgcgcaag
    79621 ctgaaacagt acagtaatta gggcctttca ggccattaat catgctgata tgaccggcag
    79681 taatgtcggc aatcatttta ggaataaaaa aaggattgaa gcggggtgtt ccatcgcctt
    79741 tggcaaattc ggagacctct tcgtaaaaag tgatcaatcc gccaatacca ctggcccaaa
    79801 ctacaccaat gcgtgaacaa tcctcctgct caaggtttaa acctgagtct ttaatggcct
    79861 cattggcact tatgattccg aactgagaaa ccaagtcata ttttctgacc tctttccggt
    79921 caaaaaactg ggttgaatca taattcttta cttcacaggc aatctgtgtc ttgaattttg
    79981 atgcatcaaa cctggtaatc gttccacaac cacttactcc gttgagtaaa ctgtcccaga
    80041 attcagggac agtgttacca atcggagtaa ttgcgccaag gccagttact acaactcgcc
    80101 ttttattcat caaatacagt ttttactttt tgttttcttc aatgtacttg atcgcttcac
    80161 ctacggtgcc gatcttttct gcctggtcgt ccggaatctg gagttcgaaa gctttttcga
    80221 attccatgat cagttcaacc tggtcgagtg aatcagcgcc cagatcattt gtgaagctgg
    80281 cttctggttt aacttcagct gcgtcgacac ctaatttgtc aacgataatg gcttttactt
    80341 tttctgcaat ttctgacatg ttttcctgat tttagtaaaa caaggcgcaa agtaacaact
    80401 tttaaacatc atggcaaaat ttaggttgag aaataatact taaattgcct ctatcttatt
    80461 ataaatctgc atcttacaca tatagcatca aaactctacc ttataattac taaaaaaggc
    80521 gaattgttgt caatccgcct tttttttatc cgatattact attaattctt aagaatcaat
    80581 ctgttgaaaa atgtttttcc atttgcatca atcctgaaaa gatacgctcc ttgtgcccgg
    80641 ccatgcaggt caatgctgat atcccgttgc tctgaatatt ttttccataa tatcagcttt
    80701 ccttcagtat cataaacata atactcgcct gatgcaggtg tatcacccaa ttggatatta
    80761 aatatacccg atgatggatt cggaaaaata tccggtttta ttgacccact ttcatcgacg
    80821 ccagtcagca ctgtcaatac tgcaccattg cttgttacac taccacaagt gcccgtaaca
    80881 acacagctat aagtgccaaa atcggcagaa gtaacattca aaatctgcaa tgtagcactc
    80941 gttgctccag aaatattgcc tccattcgaa atatttacat tgtttttctt ccactgatat
    81001 gttagggagc cacccgaagc accaactgta agcgaaacat catctccttc aaatacattg
    81061 acactggctg gctgcgaggt tattaacgtg gctgcattca ccatcaatac tgcgccacta
    81121 ctcaattcgc tgccaaatgc attggacacc aggcaacggt acgttgcggc atcactggcc
    81181 gataccgatg caatggtata ggttgacata gtagcaccag caatatttac cccattcttt
    81241 tgccactgat aagtaattgt tggatcaccc gatgcagtaa cagtaaatga aaccgacgtt
    81301 cccggacagg cagaagtgct taccggatca gtcactactg cagccgctgt accggccgcc
    81361 actgtggaat taaattccag atcatccatc cagaacataa acatatcggt tgaaacatgc
    81421 cttatagcaa gataaatggt ttgattatca tatgccgaca aactgtaggt atgctgtgtc
    81481 catgtggcag gagcttcaac aacagcggac agagcagtga aactggccgg ctgattatcg
    81541 gttgttgata tcataatctg atatgaatta tttccccagg tactgggttt gggactaagt
    81601 acccaaagcg agaagcttga attggtacct aaagccatgg ccggagatat aaaccagtcg
    81661 tcgctggtag attgatcaga gggacaaata gccattccgc accgtgaacc accatgtgca
    81721 acatcgccct gagctgcagt ccagccagag agtgatggat tcatgcacat ataagcaaac
    81781 gcagtaccct cgccagtaaa tgttgcatcg ccacttgaat aggtagcaag tccgtcttga
    81841 tctgcagtag tccacggagc aaaatcagta gtataatcgg ttgaagattc gaaatcaagt
    81901 gtaaacggat aggttgagcc acctgttgca aaagttgtcg cattgtccgt tacgccggta
    81961 ctccaggtat aacttccggt attggtccat gccttgtaat agtaagttgt tgaaggattc
    82021 aaccctgtat ggttaaatgt tgttgctgac ccataataaa tcacatttcc accaccggta
    82081 atggtattcc ccgccacata agcagtgcca tcaaccggag ttccaaaaga atttgtagtg
    82141 ttccatgcta ccaaaacggg attgctgtta ctgttgagca accagctcaa ttcaatttct
    82201 gaagaagaaa ctgcagttgc atcaaaattg cttacgtcag tgggtgttcc ggcaggcaac
    82261 tgaaatgaat aaatacgagt ttcctggcca ctggtcgaag cacaatactg accggtatgc
    82321 caaaacgtaa cgccatccgg atcgagcgaa gtttgcgaat aatcgccaaa acgattaact
    82381 ccgctttgtg aagcattacc ggcaatagcc gtactttcgg caaaagtcat agttcccagg
    82441 ggatccgatg ccaggcgacc cgtataatac aaacccggat tggttgtggt tgaagatttg
    82501 gcataacaaa gcgcaatgct gccattatca tccatagctg cactgctcat ccagcgggta
    82561 tcgctgtcgg gctgataaat tccctgttga tacatgctcc atgttccgga agtctgccgt
    82621 aactcgcacc aatatatggc tctcacgcga gggctgtttg aaatcagcac accccaggta
    82681 agcacaacac tattatatcc gctccaggag cgccatggag ccctatacat caaaacgccg
    82741 ccaataccgt caagatattg cccattgggc tgcggaacat cgttccagct tgactgataa
    82801 gaagcatcga atgaatttgt gttgacagaa gtggggccgc ttactgtaac tgaaggtgtt
    82861 gtaccccagc ttgtggttgc agtccagatt ttcacagcat cgtttccggt tccccatgca
    82921 ttatcaaagt agctgaaaaa tggcagcggt gttccacttg ccggcaaggc atcgtcgtct
    82981 gccgcatcgg caggcagcgg aatgaaaaaa gaagaaactg aaccagttga gaaattcgaa
    83041 tatgtcattc ttgccgacga acttcctgta agcatggcgc ttctttcaaa aacatggatt
    83101 ttgtctgaag actgattggc agtcatataa tagccgtcct gccagatgga aaatttcagg
    83161 taatcaggaa attcggtgga attaaatgtc catgtataat aagaaccggt gggatcggca
    83221 gtttgagaaa cagcaatgta aatttgtgta cttgatccaa attgagcaag gaaccagcga
    83281 tcggcaaatt tatcgtacat cacaatcggg tcaccggcat tggatgatga accccataag
    83341 gtacccaatt ggaatgattc ctgctccact ccggttttgt tgaaaatcct gacagttgta
    83401 gcatttacca tttgtacata atgattcggg ccaacagcac cggtgggatc cggtggatat
    83461 gccgaactgg ttacaccagc ccagttttgc aatgtggctt tggttccgac tgtccttttt
    83521 cccattgagg tctgaactat cgccggatca ttcccgtatt caggaccatc ctcggccgaa
    83581 taaacaaatg tttgcgccgg tctgtttttc ctgtcattcg aaacccaggg tatggttgta
    83641 aaatcgggct ccacataggg ttgctcatca atcagttcgc tcaaaggcct ggttacagca
    83701 aacgatgatg catacacaaa acccgactcg ctattgtttg gccgggattt ggattcgttc
    83761 tgcgctttca gcgacaatcc tgaaaaagca aaacagaaag agagaagtaa tggtaaaatg
    83821 tgaactttca tggggctttg tttttttctt gacttgttag acagattttt tcggttttgg
    83881 ttggcactat ggtttgtaaa tttttctgaa atagttaacc aactgtttct attattgcta
    83941 tttttgttct tcttataata attgaccccg ctttgtgtta tttgaaaacc gccaaggtca
    84001 ttattgtcaa ctaaccgttc actctttttg ttcatggata tttatcgtcg aaaaagattc
    84061 tggaagtggg gactgttcat cattgggctg ctgatagtga atgcatcggt catgttcagt
    84121 aataatctgg taaagaaaat tgccacagat gaacgtaaaa aagtgaaaat atgggccaat
    84181 gccattcagc agaaagccaa tctggtgaat tataccaatg actttttcaa aaaaataaaa
    84241 gaggaagaac gcaagcgggt tgaaatatgg gccgaagcaa ccaagcggct tatcaatgcg
    84301 ggcatcaacg aagacctcag cttctataca cagatcattg ccggtaacac tactattcca
    84361 gtggtgctca ccgatgaaga aaaaaatatt attgctgcca aaaatgtcga tttttctacc
    84421 gataccgtaa ataaactgga aggaaaaaga ttaaatgaat tttcgaaaat cgaacccatt
    84481 gttgtcagct atggcgaatt tgtaaactat ctctatttca aggaatcgct tcttttcagc
    84541 gaactgcgca atgtactcaa tgacctcaca gaatcattct tttccgaagt tgttattaac
    84601 agcgcaagcg ttccggtgct tattctggat tcggcccgtt caaaggtgta ttccagcggc
    84661 aatataaatc aggaaattct gaaaaatcca gatgagctta aagaactgat aaaaacaatg
    84721 gagacccaaa acccgcctat catcatcgat ttcgaggggc agggcaaaaa atatatttac
    84781 tacaaggatt cgtatctcct tacacaatta agatattatc cctttgtgca attatttgtg
    84841 gttggccttt ttcttctggc agtatacatt ttattcaata caacgcgaaa agccgagcag
    84901 aacaaagtgt gggtgggcat ggccaaagaa acggcacatc agcttggaac acccatttca
    84961 tccatgcttg cctggctcga tttcatcgaa accgacgaaa aaaacaaagt ctatattgat
    85021 gaattgcgaa atgatatcag gcggctgcag gatgtgtcgg cccgcttcag caatatcggc
    85081 tctgcacctg atctaccaat gactttgctg tctgaatctg tgtacaactc tgtcagatac
    85141 atccgcgccc gttcttcaaa aaaagtcagc ataaatatcc ttttcagaga aaatgaccaa
    85201 ctgatgctcc cgctcaacga aaatcttttt ggctgggtga tggaaaatct gctgaaaaac
    85261 gccattgatg ccatggaggg tgaaggcagc ataacaatct ctctccacaa tgcttcacgt
    85321 cacatcatgc ttgatgtaac cgataccgga aaggggttcc caaaatcgca gtacaaatcc
    85381 gtttttcgtc ccggattcac aactaaaaaa cgcggatggg ggcttggact cacactttca
    85441 aagcgcatca ttgaagaata tcacaaaggg aaattatttg ttcactcatc tgtacccggc
    85501 aaaggaacta cctttcgcat agttctgcct ttgaaataaa ctatatgaaa aaaccgcttg
    85561 cttttctgtt gataatagtc atggctgcat cctgccggtt tttgggaatg gagaaaaaga
    85621 attccccgga atacgtcgca aaaaaatttc tgacacattt tcagaagctg gaatttgacg
    85681 aggcttccca gtatggcaca gaaaatacga aaatgattct tcgcctgttc aagagtttat
    85741 cagccatggt atccgattca accaaaagcc agaacaattt cagcgatgca gttattcaga
    85801 aatgcacggt ggaaaaaaac accgcgttat gcagatatac tgccaatgga aaagaagaaa
    85861 caattgatct gatcagacag gatggaaaat ggcttgtcga tctgaaaaaa gagcagaaaa
    85921 atccgtttaa tcctgggaaa taatctgctg ctctaacagg ctacttcctc attgattaaa
    85981 acccggtcgt agaatttatt gaaagcgtca atgtaattct cttcgccctg atattcaagc
    86041 atcggaagtt tgcttttctt tgcatattca agcacgtcgg gattcacgtt ttcagagcct
    86101 acgataacac catccgcata atcgatggcg gttttcatcc agccaacata gttcctgtct
    86161 tttttaagtt ggggtaatcc gactgccgtg agatcatcca taacaatatt tgcaataacc
    86221 tgttcggaaa aatattctga aaaatgatca tcatagagcg aaattacaat tttgctctca
    86281 gaaaaaatcg gatgcttttt atagactttc tttacgtatg cagctgcaag agccgacatc
    86341 cagccatgac aatggatgat atcaggagcc catccaagtt ttttcaccgt ttcgagaaca
    86401 ccacgattga aaaataccga tcgtgcatcg ttgtcgttgt aaaaacgatt gtccttatca
    86461 tgaagaatga acttgcgatg aaaataatcc tcgctgtcga tgaagtaaac ctgcattctt
    86521 gcctgctgaa tacttgcaac cttaataatg agcggatgat cggagtcgtt aatgataata
    86581 ttcattccgg aaaggcgaat cacctcgtgg agctggtgac gacgctcatt gatacatccg
    86641 aaacggggca taaatgtacg gatctctttt cctttttcct gggttccctg cggcaggtgc
    86701 ctgcaaacat ttcccatttg actgtcccta aggtacgggg ttatttcctg agatacatac
    86761 agaatttttg ctttctccat aacaatgcaa gggctgataa ataatatgca aagttacgat
    86821 tttttttcca tatatatcaa aaaaagagtt tagttttgta ctttttttcg actgattatg
    86881 aatattttcc gtacaattaa ggaatacaaa gactggcaag aaagtttaac agataaaacg
    86941 tcagttggct ttgttccaac catgggattc ctgcatcagg gccacatttc gcttgtttct
    87001 gcatcaatgg cagaaaacga tgtgacgatt gtcagcatat ttgtgaaccc tacacagttt
    87061 aacaatcctg aagacctcga aaaatatccc agaaacgagg aacgcgatct gcaaatgctt
    87121 gaaatggccg gaaccacagc tgttttaatt ccttctgtaa gtgaaatgta ccctgagccg
    87181 gttacacgta catggacatt ccccggactt gatgatgtga tggaaggtgc aaaacgtccg
    87241 gggcacttta acggtgttgc catggttgtt tctgaattgt ttcgaattat tcagcctaac
    87301 atcgcttatt tcggcaagaa agattatcag cagctcatga ttatcagaca gcttgccgca
    87361 aatgactttc cgcaaatcac cattaaagga atgccaatcg ttcgtgaagt agacggactg
    87421 gccttaagtt cacgcaattc aagactgaca ccggaagagc gggaaatggc gccacttatt
    87481 ttcgaagtac ttaaacgtgc ccggcaactc atacatgaat tttcgcctga agatgtctgt
    87541 atacactgtg agcgtatgct cgccggtagc ggaatgattc agccggaata tttcatcatt
    87601 gctgatgcac agaccctgaa accactttca aaaaaggaag atgcaaccca tgcaacagcg
    87661 tttgtagccg catggcttgg taaagtgaga ctcatcgaca atttcgaatt attttagtaa
    87721 ttttgctgca tgaatataca ggtgttgaaa agcaaaatcc acaaggcaac ggtgaccgaa
    87781 gccgatctgt attatacagg gagcatcacc attgatgaaa acctgatgga cgctgctgat
    87841 ttgattgagt ttgaaaaagt tcatgtactg aacatcaaca acggagaacg gctcgaaacc
    87901 tacgttatca aaggggcacg cgacagcggt actatctgca tgaacggtcc ggctgcacgc
    87961 aaagtggctc caggagatac cgtgatcatt gtttcctatt gtacactgga atacaatgaa
    88021 gcaaaaaaat tcaagccatc catcattttt cccggcaaaa acaacaaact ctgagaattg
    88081 aaaaagaaaa tatttaaaat tgtccagacg cttgccttta ttggtctggg catatttttc
    88141 attttctttt tctggggaaa actcgataca gaacaacagg aaaaagtcat cgataatttt
    88201 ctgaaagcaa attatttctg ggtgctactg gccctttcgg ctggtattct gagccatatt
    88261 ctgcgagctg caagatggaa tttactcatt gatactatag aaaaagcacc accgctcagg
    88321 caatcattct ggtcactcat ggctggttat cttgccaatc ttgcagtgcc acgactgggc
    88381 gaaatcactc gcgcaatact gctttcaaag caatcgaaaa tatcattcga caaactattc
    88441 gggaccgttg ttgctgaacg tgcctttgat ttcttcttct atcttgcttt gttttttgca
    88501 gccattgcca ttttctggga caatgtcaaa acatatgtta cagagcggat catgggtggt
    88561 tttgccgaaa aaacaagtgt gttttccgga tggaaaatat ggctgattct cggaattctc
    88621 tgcgtactgt ttcttctctt tctttggatc ctgaaaagat tcagacacaa accgcttatt
    88681 cagaaaataa acagaataat ccagaacctg ctgagcggac ttctctccat tttcaaactc
    88741 agaaaaacag gattgttttt cgtttataca attctcatct gggtaatgta ctggctcatg
    88801 gtttatttca tgtatttcag tattccgtca acatcaggcc tctcagctga atcagcgttc
    88861 atggttcttg ttttcggaac catcggtatc atcgttatcc agggcggcat cggaatttat
    88921 ccattgattg tttcagaagt acttatgatt tatggagccg acattacgga tggtttcacc
    88981 atcggatggc tgtcatggac cgtgcagacc attatgattc ttgtaatggg attgattgct
    89041 ttcaccttat ttggctttca aaaaaacaaa aaagatggac tgcagaaaac tgatcagtga
    89101 gaaaatcatg tcgcgcgaac aactgattca gcgcgtaaat acatggagaa cagaacgtca
    89161 gaccatcgtt ttttcaaatg gctgtttcga catcattcat ccggggcatg cgcagtatct
    89221 ggcggcagct gcggctctcg gcagccgact ggtgatcggt gttaataccg atgattcggt
    89281 aaaaagactt aaaggcgcgg cacggccggt catttccgaa aacgaacggt gcctgctgct
    89341 cgcatcattt cagtttgtcg acgctgtctg cctgtttgat gaagacaccc cgctggagct
    89401 gattgaagca atcattcccg atgttctggt gaaaggaaag gattatacga ttgaccagat
    89461 tgtcggcgca gatgtcgttt tgcaacatgg cggaaaagtc gaaaccatcg aacttgtgga
    89521 aggtttcagc acttcgtcgc tgatagaaaa aatcaaacgg atttagcagc taactctcag
    89581 aaggagtccg aagtaattgg tgagcagtga tcagcaaaca gtgagctttt gctgtacaca
    89641 caacgaagcg ttgtttgcat ttcagattta aacctcatgt taatatagaa tttcgctgaa
    89701 tattcatcag ccaacgctca ccgctcactg ctcaccgctg aaaatagttt cgtatttaaa
    89761 taaacctcac cagcgtgatc ccctcgcctc ccatttgcag cggtgcatca gtgaattcga
    89821 gcatatcctt gtattgccgc a
//
LOCUS       DAOPKE010000003        66570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_425_length_66570_cov_11.882162, whole genome shotgun sequence.
ACCESSION   DAOPKE010000003 DAOPKE010000000
VERSION     DAOPKE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 66570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_66570_cov_11.882162"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1349)
                     /locus_tag="PKY63_00685"
     CDS             complement(405..1349)
                     /locus_tag="PKY63_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99155.1"
                     /translation="MQDGTAVVSTSGGTAPITITWDTSPVQTGTSASGLSTGNYTVIA
                     TDASGCSDTLIVFIGLSTNTYLTVSTTPEHCGHADGTATVVVHNNVGSYTISWNTTPA
                     QTSETISNLTPGAYTVTMTDSIGTYSNVAYVTLAPGPNASFYANPNPATIGGGPIQFI
                     NSSSGASNYEWSFGDGTTSVEQYPSHLYGGVGEYLVTLLATDNFGCRDSFQLTIIIND
                     LFAFYIPNAFSPNGDGHNDFFMPYGISVDLDYYEMRIYNRWGNLVFNTTQANIPWDGN
                     INGKIVKEKLQDTYTYEIFIKDASNIIHKYRGKVTLLY"
     gene            complement(1737..2720)
                     /locus_tag="PKY63_00690"
     CDS             complement(1737..2720)
                     /locus_tag="PKY63_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPE99156.1"
                     /translation="MNKIFSIFFLLLPLFVLSQTPTNQDCLGAIPVCQYTYTQSNSYS
                     GTGNYTDEISTSGCPGSCLLSGEKNDVWYIFTVQTSGNLNFNITPNSSSDDYDWAVYN
                     LTNANCDDIYSNPSLQVSCNYSATPGTTGANGGSSLNCADAMDGNDNAVIPVTAGQTY
                     ALNISNYSSTQYGYTLDFGPSSASIFDNSPPVFQSVNLPVACGATQITFNFSENVMCS
                     SVLASDFQVTGPGATYTSVSISSAACSSGADMDNTFTLTVSPAMTTSGNYQICMVSTV
                     TDACGNAAALGCFSFVVSGVSTNITTVDANCGPNGSATVTPYGGSGTYTYL"
     gene            complement(2734..3144)
                     /locus_tag="PKY63_00695"
     CDS             complement(2734..3144)
                     /locus_tag="PKY63_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99157.1"
                     /translation="MKTILKSLAVLLIVFCGSNLFGQSQNYIAFFDNTEQTVYRDNKD
                     RLVMSYKIEGPQTEAEIESLKRLYLLYNMFEEFEIKPTGEPNVWEIHEITKPAVKIKE
                     HRKLFVVAGIYTVFVDNEPYPVEGFRMKMLKNKN"
     gene            complement(3319..4323)
                     /locus_tag="PKY63_00700"
     CDS             complement(3319..4323)
                     /locus_tag="PKY63_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99158.1"
                     /translation="MKTAVVILNWNGKGWLEKFMPGVIASLPATAELIVADNASTDGS
                     LELLKEKFSSIRLIEFEVNHGFAGGYNEAFKLIDAEYFVLLNSDVETKPGWVEPLESF
                     MDAHSEYAACQPKLKWYGHDNMFEYAGASGGFLDKNAYPFCRGRIFSTLEEDKGQYDD
                     IIDVLWATGACLFIRKSDYDAIGGLDEKFFAHMEEIDLCWRLWNAGRKIAVVPQSQAW
                     HVGGGSLPRSSARKTYLNFRNNLLLIYKNYPASQLSKVLRRRFFLDFIALGMFILQGH
                     FADAWAVIKARRHFSSMKKQFVKSTTGSFPATIYAGNILSDYYFRKKKHYTSLTNQSK
                     "
     gene            4604..4996
                     /locus_tag="PKY63_00705"
     CDS             4604..4996
                     /locus_tag="PKY63_00705"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPE99159.1"
                     /translation="MAELKTKPENVDVQSFIDELPDEQREDSNYLLKTMQKISGEKPE
                     FWTGGMIGFGRYTYQGKSCSGEWFPLGFSPRKNYLSIYLLGYIDESDPLLGKLGKFKA
                     GKGCLNIKRLSDIDKNVLEQIIKNNKKK"
     gene            5004..5882
                     /locus_tag="PKY63_00710"
     CDS             5004..5882
                     /locus_tag="PKY63_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99160.1"
                     /translation="MNPGDILQVNLKKHIELSETEQYWVAETVDGQRFLIDYFQYVHY
                     GFTTGHSIQVQVDKINCSGKVFVEPNHPVYEREKTYVFSIDSVLSKNENYFSLIVKDV
                     FDNNISVQFHAKGLDRIPESVELLVIGLRKGLPVLLDPMLREQTPFKENEVYSFMVVD
                     KIKMNHEEYFQLEDEFHRIHLLPARFYEKYPIVIGKPLQCYVVRIDQNAELKLEPVHP
                     LFHLGHELDLYFVGEYHGDEYIGQRHKLYLLHDDEGNEFFMSVRFMEGREIPKRLRCR
                     IEKLKKGQPLVEPVDV"
     gene            5988..8147
                     /gene="rnr"
                     /locus_tag="PKY63_00715"
     CDS             5988..8147
                     /gene="rnr"
                     /locus_tag="PKY63_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPE99161.1"
                     /translation="MSKKKTNNTNKNQEDNIIAMNILAIFRQNPYMLFNYKQIAGRLG
                     INRGPEREKMCAAIDRLVSQELLLVRNRGKYVLNPEAIEVDENASEIEGVIDITANGK
                     GFLLMPDKDDIAIAQGDLNQALTGDTVLVRLFPRRKNRRPEGKVIRVVKRKRQQYVGI
                     IQVGAKMSFFVPDDRKIHIDFLVPRENLNNAKNGEKVIVEITDWPPQSRNPFANIVTV
                     LGQPGVNEVEIQSILANYEFPLCFPKHVEKEAERIPVEISAEEIKKRKDFRTTFTFTC
                     DPADAKDFDDALSLKKLSNGNWEVGVHIADVSHYVKTGSEIDKEAFDRATSIYMVDRV
                     IPMLPEVLSNNVCSLRPNEDKLCHSVVFEMNEEAKVLKYWIGHTVINSDIRYNYEEVQ
                     EIIETGKGKYSEEILLLHKLATIMREERNKKGSINFKSTEVKFKLDDDGKPLEVYVKE
                     QKESNMLIEDFMLLANKTVAAHIGMKTEKNKNPKTFVYRIHDEPSPEKLANFSEFLGK
                     LGYSINITSKQNISYSLNNLFNKVSGKAEETMIETIAVRTMAKAIYSTNNIGHYGLAF
                     DYYTHFTSPIRRYPDLMVHRLLDWYAASKPSVNEAEYEDMCDHCSDMEKKAADAERES
                     VKFKQMEFMADQVGKTFDGVISGVSKWGIFVEISYSHAEGLVRLEDMKDDFYYLDEDN
                     YMVIGHRTKTTYRIGDKVNVLVKKIDIGRKQMDLAIV"
     gene            8397..9629
                     /locus_tag="PKY63_00720"
     CDS             8397..9629
                     /locus_tag="PKY63_00720"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPE99162.1"
                     /translation="MREYKIFHGKTLGILSGGGDTPAINSSIENIRNRAQLLGYKVFG
                     IRHGWKGLLGDGDVVDLTNQPYDGIYGGTALRSSRTNPFPSKKNPESRVPQILKNIER
                     YKLDVLVTIGGDDTNGAAKRLYETEGIPVIGFPKTIDNDLRTRTVHEYSNKKIEAVLC
                     PGYPSALKGVMEFTRRIKTTAESHSRILVLEIMGRDAGWLTGGAAYGQADIILVPEYA
                     MDKSRKDFFFEFVKEAFMRSPKKSLIIAVSEGLRWYDDQSGKTDVVYASTEVDEYGHP
                     RLGGVSGVIASEISNKLGIEARAQTVGYYARSGECRLYDRKLTATLADKVVDLLLRED
                     YGKMPVISKMVSYPELEEFNTGCIDMGNVGNKPLPEEYYDVDEFRFTDAYYDFMHHIL
                     GDRTFIDFKYNFPVVIPE"
     gene            9629..11041
                     /locus_tag="PKY63_00725"
     CDS             9629..11041
                     /locus_tag="PKY63_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE99163.1"
                     /translation="MKIFFWILLFPVVLQAQTLSVSTDSLMKHVNYFASPALNGRLPG
                     TDGFLKAVQYSEYYFEQHELTQVLNDSWQQNIPIETNVIDSARVMIETGRDMLELIPG
                     VDFSVRGYSGSGNVTAPVVFCGYGFDSEKYSDYKDISDKDLDVKGCIVMVFKSNPPFI
                     ESLPDYSIRKVADIAYKHGAVGVIFVSPPNQKNPQKPIGSMMHGPGAMHADLPQIQMS
                     VEKADYVAGWHSLLELQKRIDTLQKPLSMGCQRHVNIQVNATYNANGNTTNIIGMLPG
                     SDPVLKDEYIILGAHLDHVGAIGNTVYYPGANDNASGSAAVLELARIFSADRSKLKRS
                     VIFVLFGSEEKGLDGSTCFVNNLPVPKEKIVAVFNMDCIGCGDSLMAGNGKSCPDLWN
                     MAIGIAQQNKWAVSQRTWDGGGADLKPFYDAGIPGLYFVNTNAHQQLHLPSDTPEILN
                     QPQFSRMVMWVEAVTRKLLM"
     gene            complement(11070..11891)
                     /locus_tag="PKY63_00730"
     CDS             complement(11070..11891)
                     /locus_tag="PKY63_00730"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPE99164.1"
                     /translation="MKTRNLISAAAFIAFFFAFIAANAQYRTVQNTAYKRGEKLTYII
                     YFDSFLTGKIKAAYGTFEVLPETETVGGRTCNHIVASGATFKKYNWAIYVNDRFDSYV
                     DEQGQFPLLFLRRAHEGNFTANQDIIFNHSKNLAQFKDNKKEKTINYYIPTYAQDMIS
                     AGYYARNLDLTKFKAGENITIPYVFEDSLFSTTLVYDGIANLKMGLGTFSCIKLKPKV
                     LVGTVFGEAYPLTIYVTNDKNRIPLYAKSSILIGTVKFELISYSGLKNPLDSKKK"
     gene            complement(11888..12136)
                     /locus_tag="PKY63_00735"
     CDS             complement(11888..12136)
                     /locus_tag="PKY63_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HPE99165.1"
                     /translation="MLTTCSYMKKLIRNIINLPSIIGMMVGAGAGYLYYYLVGCNSGS
                     CPITSNPWGSLAWGLIMGWLVGSMFYKDKDSNTKNSQS"
     gene            complement(12174..12584)
                     /gene="trxA"
                     /locus_tag="PKY63_00740"
     CDS             complement(12174..12584)
                     /gene="trxA"
                     /locus_tag="PKY63_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPE99166.1"
                     /translation="MKNKLLILAGTVAAFVFVGLNNAQAQTEEKKTHTLTITTENFDE
                     TVKKGVVLVDFWATWCRPCVMQGPIIDHLADSLHGKVKIGKVDTDKNRALSQRFGIQY
                     IPTTIIFVDGVAVDKASGLQSKEVLIKRLTPYLK"
     gene            complement(12696..15065)
                     /locus_tag="PKY63_00745"
     CDS             complement(12696..15065)
                     /locus_tag="PKY63_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99167.1"
                     /translation="MKKVVVFLFFFTMLFMANSQKTTLSGTVFDKSTGKTLPGANVVI
                     SGTLNGTFSASDGSFSVSTRTSFPLTVIVSYMGYNTDSISLQSVPESSLKITLTPAAV
                     ISDEIVIKAIRINNQDAAACSNLDPVDIAKINTGTDIPVMLDALPSVTTSSDAGAGIG
                     YTAFRLRGTDQSRINVTINGVPYNDPESHNVYWVDIPDIASSSESIQVQRGVGTSTNG
                     SSSFGGSINISTHSYKPDPFATLNFKAGSFGTYGSTALFGTGLLNGQWTIDGRASIMH
                     SDGYIDRASADIQSLQTNVAWYGEKTLVRMMLMHGKEKTYQAWEGVPKDSLETNRTFN
                     PYWYENEIDLYTQNHFHLVISNQHSQKWTSSATAFLITGNGYYEQYKDEESFSDYLLP
                     DLIIGGDTITETNLIRRKMMDNYFYGAVFSANYDNRKKLQINLGGNLSRYDGDHIGNV
                     IWMQYSGDSDINHEYYLNNGLKDDISGYAKLRYSIKKHTTIIADMQIRNVRYQVEGDE
                     DDKKNLSIDKSFLFANPKASILHAFGKHSVYGYFGVAGREPNRYMMVDADSGKMPVHE
                     ILFDYEAGYKYTADKWNINLNLFYMNYRNQLVQTGEINNIGSAIYVNVPESYRAGIEG
                     IWEWKVNEKFDFSGNATYSVNKIKDLLVFIDDWDTWSQHTEFYKTSDISFSPAIIGSA
                     RFGYNPIKNLRFTLSGKYVSRQYIDNSSSIERSLDPYKIFDLGAEYVLKPKFVKEVAF
                     RLTLRNIFSEEYESNAWVYRYYYGGEHYVMDGYFPQAYINWLGSVSVKF"
     regulatory      complement(15113..15209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15340..17397)
                     /locus_tag="PKY63_00750"
     CDS             complement(15340..17397)
                     /locus_tag="PKY63_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPE99168.1"
                     /translation="MTKVLTFALLLLLTTSLTSAPVSLHNAWMAAINVMKHYNKVPDN
                     LMMEHVAFDSQSQNPEFYIFNANEFGFVIIAGDDAVSPILGYSTESSFTGENMPPSLV
                     ALLNAYSQQIRHIRENNIKADHSINEQWTELIYGIIEKDKGYSVNQLLTCQWDQGVPY
                     NALCPYDPAGPGDRVYAGCVATAMAMTMYYYRYPVQPSGYHSYFSDYGQLSVDFSQSH
                     YNYEQMPYRLTSANYDAAKIQYDCGVAVDMMYSPNGSGAYMDDALNAMKDHFGYNPSA
                     TLEYKNDYSDTDWITLLKSQINAGHPMPYAGYDVSSGHAFVCDGYSDDMFHFNWGWSG
                     SYNGYFLISNLNPGYNFSSGQQAFINCYPAAVTYPASCGNYQMTASSGSLEVGHGLSD
                     YSNDQNCSWLIQPSDSIQYITIEFRYLRTEAGNDVVTIYQGSDESAPVTGTYSGISGD
                     FSVQVPGNRAFITFQSNNTITDDGFHADYYTYTPAFCSILDIRNDQAGTVNDGSNNYS
                     YNNNSVCRWRIEPPGAGGIMIDFTEFSLEPASDVLYFYQYPSYILVDSLTGNAIPQSF
                     FINSPKTMVVFKSNEANADDGFTFNYHGVATGMDDFQNSSMYISVNENSFPVLTVNQF
                     PAGTYSIELCDITGRLLYSTTKNISGLKEQTEIPYMPENAGLYLITLKGNNISQTLKY
                     FAR"
     gene            complement(17431..19503)
                     /locus_tag="PKY63_00755"
     CDS             complement(17431..19503)
                     /locus_tag="PKY63_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPE99169.1"
                     /translation="MKKISTTFLFFLTFCSVIQLSAQVVTEKTARQAAFSILSTKNAI
                     MPATAAELILISVPGTSVVNPEIFIYNNPNGGFAIISGDKSATPVIGYSTRNSVPQTA
                     WKSNFAWWIQNAIEQIQYYRQHATKSTSEIDEEWGFLLSGNTTNNPFHSSKAVLPFLR
                     STWGQDGVFNDQCPEDANGEALVGCVATAMAQVIYYYQFPQTGYGSHSYNAYGYGTQS
                     VNFGASTYNYSEMSGDPGFPMPEVAELSYHCGVAVDMGYGYDGSGAYTWDVPDALTTY
                     FRYESANYRSRMTYNSTTWATMMRNNLDLLRPVIYSGSGDGGGHAFVMDGYEGTDFFH
                     FDWGWDGYANDYFYLNALNPAGSDFNDSHQCVESIYPPSASYPYGCTGTKTLTSTFGL
                     IEDGSGPTENYEANCNCSWLISPGPQCDYFKITFEELDLATDDSVKIYAGADATATVA
                     GAFSGSTVPAYFNVNSSQVFIQFVSNSTNHSKGFLLNYVGHIPLGCSGITSMTNPTDT
                     FDDGSGTNTYGNSSFCRWNIQPAGATSISVHFIDFDMADEFDLVKVTDAITSTVLGEF
                     HKGDNPTIVSAATSKIQVLFQSNTSITGDGFKVFYTTTTDISERPEGYSLLVFPNPAS
                     NVLNIIPGNLADGASVVIYDFSGRCVFNQKTDGSTMMIPIDNLSSGLYSVGLISENGN
                     IWQKVVIE"
     gene            19622..20884
                     /gene="hutI"
                     /locus_tag="PKY63_00760"
     CDS             19622..20884
                     /gene="hutI"
                     /locus_tag="PKY63_00760"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPE99170.1"
                     /translation="MKLIFNAAAVAVIENAPSPNPVAGNLMKQTNVIENAYLLIDNNR
                     IHSFGKMSDLKSVRQQFSISEEIDASGGFVLPGFCDSHTHIVFAGSREMEFVDKINGL
                     SYEDIAKRGGGILNSAEKLRHTSEDELFEAARKRVIEVISYGTTALEIKSGYGLTPES
                     EIKILRVIKRLKEHFPIPVKSTFLGAHAIPKHYRDNRQDYIREIIDVMLPQIADENLA
                     DYIDVFCDEGFFTIEETSAILKSAAAYGLKPKIHANEMANSGGVQIGIKYHAVSVDHL
                     ERIENAEIDALLASNTMPVALPGASFFLRIPYAPVRQMIDSGLPVVLASDFNPGSSPS
                     GNMQFIMSLACIYGRMLPEEALHAITINGAFAMEIQQDYGSICPGKMANLIITKEIPS
                     LAFMPYYYGFNKIDRVIINGELFDPSKY"
     gene            20902..22596
                     /gene="ftcD"
                     /locus_tag="PKY63_00765"
     CDS             20902..22596
                     /gene="ftcD"
                     /locus_tag="PKY63_00765"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPE99171.1"
                     /translation="MKQIIECVPNFSEGRDLTVIKQITDVIESCEGVRLLDVDPGAAT
                     NRTVVTFVGTPDEVIEAAFQAVKKAALLIDMSKHSGAHPRFGATDVCPLVPVANITME
                     EVTEYARKLGKRIGEELNIPVYCYEDAAFTKERRNLAFCRSGEYEGLKEKIVKPEWKP
                     DFGPAEFNCRCGATAVGARDFLVAINYNLNTTSTRRANAIAFDVREKGRPKREGNPVT
                     GKVIKDEKGNDVIIPGTLKATKAIGWFIDEYGIAQVSMNITNISTTPVHVAFDEVCRK
                     AADRGVRVTGSEIVGLVPKKVLIDAGKHYLAKQQRSLGVSESELVKIAVKSMGLDDLK
                     PFNPQEKIIEYMLEAESKEKKLVDMTCVGFADETASESPAPGGGSISAYMAVLGASLG
                     TMVANLSSHKPGWDERWEEFSRYAETGQKLKDELMHLVDEDTRAFNKIMNALGLPKGT
                     PEEKASRKAAMQAATQYATEVPFKTMQKAAEVLPLCKAMAETGNPNSVSDAGVGALAA
                     TAGVRGAFLNVKINAGGIEDKAWAADIIAKAQKVHDDALTLEAEIMTQVEKVIAKA"
     gene            23317..24936
                     /locus_tag="PKY63_00770"
     CDS             23317..24936
                     /locus_tag="PKY63_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE99172.1"
                     /translation="MIAASNISIRYGKRVLFDEVSVKFTPGNCYGIIGANGAGKSTFL
                     KILSGEIDAPTGQISITPGERMSVLKQDHHEFDNFTVLETVIMGNKQLWKIMQQKDIL
                     YAKPDFSDADGVKAAELETKFAEMDGWNAESDAASLLSALGVKEEDHDKRMKHMPAKE
                     KVRVLLANALFGNPDILLLDEPTNDLDVATISWLEEFLINFRNTVIVVSHDRHFLDAV
                     CTHVADIDFGKITLYTGNYSFWYESSQLALRQRADQNKKTEDRRKELQDFVNRFSANA
                     SKSRQATSRKKLLEKLVIDEIKPSTRRYPGIIFKADRECGDQILHADNLSKQSSEGLS
                     LFSNVSFTLNRDDKVAVLSRNHHAVSAFFNILMELETADRGAFQWGSTISKSYLPANN
                     DPYFKNPMTLMDWLQQYSTVEDDDSYIRGFLGKMLFSGEEYLKKTSVLSGGEKVRCMV
                     SRMMLTNANCLILDEPTNHLDMETITAFNNALKDWKHVALFSSRDHEFTQTVANRIIE
                     LTPRGIIDKLMTYDEYLEDKSVKALQEKMYS"
     gene            25153..26370
                     /locus_tag="PKY63_00775"
     CDS             25153..26370
                     /locus_tag="PKY63_00775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPE99173.1"
                     /translation="MRLVKGSDAHIGSYSYPLELKNETGFKMPVDSARCKTGYFVINP
                     LLAVFENRNSSDSARKVFENILSYIQMDTLQFVTNALITNRIYFCSWIDGDIKKVVVD
                     TDCDSSFRNEEILTYNLSNGLYDFGDSNLVFSDCDLTLRYDSLNIHKQKTIPIRVCLN
                     ENIDGKLMFDYDSLEVYISINACQEGRFIFEGDTVDIVMNPNCFDQFYPQNSIYGVYS
                     YRYLGYDYLNYITNDEIRLFNKLFVIEKYDYFTRVLKVRFAGSDSVGAYQGNIFPVTP
                     GLEKFDYYSLVFFTGSWCGPCKHLLPGLKELHKNNPEFEIFNVNNERDSIDYFKYLSQ
                     YGIDWPVIYDQDLANVDSYYWQSYNINSIPTLFLINPNRKILDVQTGLHNCLQLLKNL
                     KENGIEYYETTGP"
     gene            complement(26524..26919)
                     /locus_tag="PKY63_00780"
     CDS             complement(26524..26919)
                     /locus_tag="PKY63_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99174.1"
                     /translation="MKKFLLLIALTLTFSFVFTACEDDTDDPSTTDYAIATSSWGKLT
                     LTLVETGGPTNEWYFASTSSSGMAALTGVDDFSFTYSKKGINSSELTFDVGGSDKYVM
                     TWTSASAGTFQESFNGTAGNPGSFTIELD"
     gene            complement(26982..27530)
                     /locus_tag="PKY63_00785"
     CDS             complement(26982..27530)
                     /locus_tag="PKY63_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99175.1"
                     /translation="MPKPDKTFISHIRQLSTAELQEIVLKLITKDKFAYDFVSSQYIN
                     KDFGETELFEKTKKELDLVFTKSHKGFSFQLQLANMLAECIRKINEFTKVSKNKVLEA
                     ELVLFVIEVPFSNPDKLLGTCFTTLDSKVAMLLNRLINLVTKKMHEDYRIEYVGKINR
                     YLEILHSTSNHLDSVYKMPKKI"
     gene            complement(27857..29815)
                     /locus_tag="PKY63_00790"
     CDS             complement(27857..29815)
                     /locus_tag="PKY63_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99176.1"
                     /translation="MFCLFFIASSNFNGFSQVDTSRSQNDTIQKINLDSVIISAQVAP
                     TVQSELLRVVHVIRAKEIQLAPVSDLGALLEYVRGIDIRQRGITGAQSDISLRGGTFD
                     QVLVLLNGINISDPQTGHHNMDIPVDITAIERIEVLQGSGARIFGPNAFAGAINIITS
                     SPQKALPSVSLRGGLYGTGELSVNLPFRTKKMYHMVSLNGSTTDGYTENTDFKKAGAY
                     YRMNATIGKTKTDVQAGYINKGFGANSFYSPKYPNQYEATSTLFASAGLTDVNAQKWK
                     ANVYWRRHYDRFELFRNNPPAWYKNHNYHLSDVAGASVNKTFSWKKHKTSLGADFRME
                     HIFSNVLGTAMNSPIAVAGTDSAYYTKSAQRNGFSIMGEHRYATKKFSISGGALVYVS
                     NQLEHDLSLYPGIDLGFQISKPLRIFATANRSLRLPNFTNLYYSSPTNIGNPLLKPEE
                     AISIEGGFKWDRKNILAEVAGFQRYGINMIDWVRDDTATVWECKNLTKVNVSGIEAGF
                     TMLPEFFGKKFFLQRVSVNYTFLVSNKSSEGFVSQYVLDYMKHKLDIKATHRIFENAG
                     LTWQLGWQDRAGGYTPFSEGVYLAEIPYQPVWLANAGVFYQWKQLRFTVNATNLFNTE
                     IIDHANVSQPGFMLMGGISWKYERRNTK"
     gene            complement(30006..30398)
                     /locus_tag="PKY63_00795"
     CDS             complement(30006..30398)
                     /locus_tag="PKY63_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99177.1"
                     /translation="MKILFIAIVYIFIFVPYGTTQRNQFRCDIVLSVEQGRIVKAQNN
                     GAESMTFRFTYITEGKNQSGIIVSSKKECSDYIELKPKETKQILTAPIDPQNKIIYSF
                     RHVIITECSATKPPVNSRGINTRMNGGN"
     gene            31025..32800
                     /locus_tag="PKY63_00800"
     CDS             31025..32800
                     /locus_tag="PKY63_00800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPE99178.1"
                     /translation="MSGKTFAEKIFNAPAGSIVFAKPDIVLSHDNTASIAGTFKKMNG
                     EKVAYPDQILIVLDHNAPPTTSGLANDYQTIRNIVKEQGIKKFHDVGKGICHQMMALH
                     ARPKMLVVGSDSHTCTAGAFNSFAAGIDRTETAGLWKAGETWFRVPDSVKITLTGKFN
                     QGVYAKDLALWIIGMIGSAGADYMSIEYHGEGVKTLSVADRMVLANLASEMGAKNAVF
                     PADEVLEKYFGQKFEGLWADVDAKYIREINIDLTTIFPVVSAPHNVDNVKAVSEVQGT
                     ELQQAVIGTCTNGRIEDLREAAAILKGNRVPDFFQLLIIPASQEIYSQALHEGLIEIF
                     MAAGANILTPSCGPCLGTGQGIPADGFNVISTANRNFLGRMGNKNASIYLASPATVAA
                     SALAGKITDPRGIHGNDKFPYSIEQSPTVSIPEGENRLLGNTWNYTDVNNLNTDQMFA
                     GNLTYNIKSSEGDKIMPYLFKGFDEAFAEKVGAGQLIMAGANFGCGSSREHPAVGLAF
                     ADVKAVICKSVNRIFYRSSINQGLPIIVLPDAVDAYKAGDAVELDFAAGKLTVGDKVF
                     TFNPLPEKLMNIFANKGLVNYYKKN"
     gene            32932..33564
                     /locus_tag="PKY63_00805"
     CDS             32932..33564
                     /locus_tag="PKY63_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPE99179.1"
                     /translation="MHYYDIHTHHESQSPLVTGILNVIPGQSVPESFFSAGIHPWYAK
                     DASTANLEQLSTNPYCVAIGECGLDSVKSAISFDEQKILFRAHLELARQFPKPVVLHV
                     VQSTNELFQLVKEFPELVYIWHGFTGNEEIVKQFLRFNMYFSFGQRGLKKPESWLSMP
                     EGRLLCETDDSDIPIEQMYAELAAIRNESVEQLSKIIENNVFRIFGHELE"
     gene            33551..34276
                     /locus_tag="PKY63_00810"
     CDS             33551..34276
                     /locus_tag="PKY63_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPE99180.1"
                     /translation="MSWNERTELLLGSDAVEKLVQSHVLIAGLGGVGGMVAEMLVRAG
                     IGEITIIDADTIHESNRNRQLIALSSNDGHRKAEEWHKRLLDINPELKVHVIAEFIRD
                     KAIPELLENKFDFVVDAIDTLAPKVFLIYHTMQKGYPLVSSLGSGSKMAPEQIRICDI
                     AESYNCKLGFYVRKRLHKLGIRSGFDVVFSPEPQKENSIIPDESDEAIKSTIGTISYM
                     PNAFGTFLASVVIRRLLGIEINK"
     gene            complement(34284..34838)
                     /locus_tag="PKY63_00815"
     CDS             complement(34284..34838)
                     /locus_tag="PKY63_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99181.1"
                     /translation="MQQGRAQFFLEKVATSFYTDYIVTDTSGMKISLPYEVQKCLFSP
                     RFVSLCDSNFLYYSDADTVFEYDISHRSKREILIVRNGPHTLCGIAWSPDSSLALVLS
                     ITPSDAENNSCDGLLFLITADGSLPPRKLKAPVNYFVIKECESIPGRDFYFIDDNTVV
                     YKKHLESLTEPGKMVTVNISEFRY"
     gene            complement(34886..39226)
                     /locus_tag="PKY63_00820"
     CDS             complement(34886..39226)
                     /locus_tag="PKY63_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99182.1"
                     /translation="MKKQYAVFVTMIFSLVCFSSFAQQDYSLSTFAEPSQNYYCFGSP
                     VYNHVILADSGGTGISAGALNIHWTVTNGTSGLLNGPATAISPDEKDTLELDTFIFAR
                     PGAYLLTVYLDSADTNKSNDTLRTWIHISLNGLYTVDASQPATGNNFQSIAAACDTLS
                     DGGVCGPVIIEIADGQYTEQARIDRVYGVSPINNIVLRSASHDSAAVKITHMPTDASD
                     NYTLSINATSFLTIEDLTLASSGNTSFSRVLTIDSTCAYNHYRNLHLTAPAATVNSNA
                     LAVIYSNPGSMTNDSISIFENNRIENGAYGMYLYGASSSNTEDSLVIRKNNIINPYVS
                     GIFMYNQSNTIIDSNVVVTNAVTSNFSGFYLRYGKGNVVVTRNTIMADGAAGDMLYMR
                     DFNGTSANPVLIANNFIYQQNNTATLRGIYPYNCNYVDVAFNTIRIQSGSTTAGRAIY
                     INSSTGYGNIRILNNIFENNGGGYGIEISSTSITNNYVSQCDYNNYNVSGSYLGKYGS
                     TNCTDIAAWRTAATAAGFDTHSIEIDPLFAGATSPEFTNAALNNLGLPMTTVTEDIMG
                     TARNLTNPDMGAYEFELLDHDMVILSIDAPVSGCGLGSTETVTATFFNNGSMPESNVV
                     AKFSINGGSTWSSPENLPAISSGDTITYSFTNSADLTTPGLYNFQVQINPLSDENPNN
                     NIDSVLISSLTTINIFPYVESFESGTGSWVSGGTNNSWQLGEPENTIIDTASAGLNCW
                     VTNLTGTHNLNELSYVESPCFDLGSMSDPWLEMDAWYEAEGGWDGAQVQYSTDGGNSW
                     QTLGNYGDANWYNDYDIDGIANNAPGWTGADVNGSNGWITMQHSMFNLTNYSGIRLRV
                     LFGAGSFNNDEGFAFDNIRISDRGNDVEISIITAPESNCGLGINVVSFDAINHGTVTV
                     TSLPVRISADGGATWVNDTIATNITSGTTTNVTTNQTFDFSAAGPHTIIVKSMLPLDL
                     NTANDSAAKTIISQPLISAYPYFDDAESIQVWSTQDTLWTKGVPAGTTINTAFSGSNA
                     YATCNQGPFSGTPTSIIESPCFDMTNMSLPHVEFKYFANSDTLFDGTTFQYTTDGITW
                     NIIGNAGDTLNWYNCEHISALATLADSTGWTGESQSTWKLARHDLSMLTGQPWVRFRF
                     VFSAMNGGNDGFAFDDFSVYENPVYDIAMLSADTLSDACEHGIDSITIRLQNTNFANT
                     IPAGDTIFVALNEGWLNVVMDTIVLSAPFLPQQIITHTFSAPVDMTVNPYTYYIEVQL
                     YNSHDADSSNNAIMQIIESFGYPTVTLAADTTFCGDGSILLDAGSGADTYLWSNGSTT
                     STLLVDTALTNGYGVFEYSVIVTTNGCASSDTTSITFIDCTGLIENEDPIKVYPNPAQ
                     DNLYIVLPATANQALLTISDMSGKTVLQKAINCEFAGNINTSILAEGVYLLRIISATS
                     NHTQQIIIQR"
     assembly_gap    39279..39378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39379..39830)
                     /locus_tag="PKY63_00825"
     CDS             complement(<39379..39830)
                     /locus_tag="PKY63_00825"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPE99183.1"
                     /translation="MIGGIQKAAQLIRESQKPAVFTGAGISAESGIPTFRGEGGVWTK
                     YNPEVLELNYFYSHPAESWKVIKDLFYSFLQNTKPNAAHYALAEIEKIKKKLLVITQN
                     IDHFHHMAGNTDVIEFHGTTAQLLCEKCHAKYPADEKMLDKIPPVCPS"
     gene            complement(39880..40119)
                     /locus_tag="PKY63_00830"
     CDS             complement(39880..40119)
                     /locus_tag="PKY63_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99184.1"
                     /translation="MNEEINWVFLRSFVWPHEYYMLQAFLESNNIRINMCDEMTINVD
                     PLLSNAIGGIKMYVAEEDFEKATGLMTEFDKNRAS"
     gene            complement(40188..40850)
                     /locus_tag="PKY63_00835"
     CDS             complement(40188..40850)
                     /locus_tag="PKY63_00835"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPE99185.1"
                     /translation="MKIFVVIFALLLFFGTTAQTHVFKPLPYAYDALEPYIDAKTMEI
                     HYTKHHRGYYDKFMAAIKGNHHLESLSLPEIMARMNEFPAAVRNNGGGFWNHEFFWES
                     MTPGGSAIPEGALKNRINAQFGSMEKFIEEFKNAGLSIFGSGWVWLIQEKGALKIVTT
                     PNQDNPLMNVPAGTVNPLLALDVWEHAYYLKHQNKRAAYIDDFFKVIDWIAVSKRFNE
                     KL"
     gene            complement(40858..41508)
                     /locus_tag="PKY63_00840"
     CDS             complement(40858..41508)
                     /locus_tag="PKY63_00840"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPE99186.1"
                     /translation="MKKLEILPEFRLDFPYNLPTLDYEYSVLEPHIDARTMEIHHTKH
                     HQAYVDNLNKALGDDALISKPLLHLFDKADKLAPAVRNNGGGHYNHSLFWKILKINNG
                     SKPEGDLMKHITQSFGGFEEFRDEFGKAAASRFGSGWAWLSIDEENNLFISSTPNQDN
                     PLMNVAEKRGYPILGLDVWEHAYYLHYQNRRPDYIKAFWDIVNWSEVEKRYLSIIK"
     gene            41925..42488
                     /locus_tag="PKY63_00845"
     CDS             41925..42488
                     /locus_tag="PKY63_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99187.1"
                     /translation="MRLFFKSALLVAILVSTSFTAQAQHVDTTDFTPGYPIVLRGIVI
                     DKVTHDSVPLAIVELLINSKGIHATRSDFDGGFVLSLCSGRLTKGLLRIQVTVKGYQQ
                     DIFEFKVEGDTSVRIELTPDLEGKITEQEVWMKFDDVIEAKYDIWGGYEKFRHCDGRI
                     KTYDEIIESGEKWLGWEPYDFDIFDPE"
     gene            42639..43841
                     /locus_tag="PKY63_00850"
     CDS             42639..43841
                     /locus_tag="PKY63_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE99188.1"
                     /translation="MKRFLSEAIQLGHAKYEQSVSRQQVIYKKSEDIRTDFSRDYNRI
                     LHCNAFRRLKHKTQVFYATMNDHICTRIEHVHHVEAVSRTICEELGLNAELGTAIALG
                     HDLGHAPFGHEGEKVISKLSKEFLQHSFWHERNSLRVVDFIESLPDLQGVEQPLNLTY
                     AVRDGIISHCGEVDQNGLKPRTEAIDLHSIRYPNEYPPYTWEGCVVKIADKIAYLGRD
                     IEDAQRLNILTNEQVRELNDLIRQKYPSVTESVFTTSLMHNFIIDLCENSSLNDGLML
                     DSDTFGLMRSIKDFNYQYIYNHPRIRRYNRYVELIISTLFQFMLDLYDGENTADNIEK
                     ECEQYPELQNSFGEWIRKYTKQNGNHSAFAIFDLTSKESYIDSVLTYLSLTTDQYAMR
                     LFGEVIHF"
     gene            complement(44319..46643)
                     /locus_tag="PKY63_00855"
     CDS             complement(44319..46643)
                     /locus_tag="PKY63_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99189.1"
                     /translation="MKYLIFVFIFIGFINTSSAQFNSSFKRLYEMADGYIYAENYIDA
                     LPCFQKLDSMAPNNPNIQFYLGMCYLKSFDEKDKASKYLELASQNISTDYYGNFNEKT
                     APVFTYYFLGKAYFYEQKFDLALENFDKFRYYLTPEDDELIKDVNRQIALTYNARNLI
                     ANPIQVRIENLGKIINSEYPDYAPVMSPDQHFFIFTSRRKGSTGGKKDQTGKYFEDIY
                     IAKCDENNKTFSNMQMLPGNVNTSGHEASISMSWDGSTLFIYRDDKGDGNIYMSKTED
                     GQWSAAEKLGGEINTKYYENHACLSPDGETLYFVSSRPGGVGGKDIWMSKRIGHNKWG
                     KAVNLGPSVNSEYNEDSPMILSDGKTLYFSSEGHECMGGYDIFYSILENGEWSFPRNI
                     GYPLNTVNDDVFFIPTLDGKQAYYASEFGECYGDLDIYRVMVLGQTEQIAILNGLVKD
                     TVMNVPLQSRVTVFDAVTNEIYLQTESDKESGNFSATLKSGHKYKVVITTAGGMQIQD
                     LLDIPYSGNDELVFYKPYYFGHTVAIEPDTILERINVGQRMGDRFVLRNVYFDFDKST
                     LRPESKTELDRLVSLLIALPDLKIEISGHTDNYGSRSYNKNLSENRAMAVVQYLVSAG
                     IDAQRLTYMGYGFDQPIATNETDAGRQLNRRTEFRIAGSVSDGMTLGHYLTEANLNLT
                     NNISANRYNDVLIDYNPRWYIIGGSFMFLKNAEKFRDELRAGGYPSAEIVGQNSTGSY
                     RVSYQSFSSKDDALKTLEKMKEEACNEGLWILQK"
     gene            46833..47069
                     /locus_tag="PKY63_00860"
     CDS             46833..47069
                     /locus_tag="PKY63_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99190.1"
                     /translation="MKLRFVYTPKPRQFTYKPQFFKPEEDGKKIFSTEGPGKMTHEAY
                     RRYRRDTSKSKQKRNQSVLIYVAIILLLLYFIFF"
     gene            47077..48882
                     /gene="mutL"
                     /locus_tag="PKY63_00865"
     CDS             47077..48882
                     /gene="mutL"
                     /locus_tag="PKY63_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPE99191.1"
                     /translation="MSDIIKLLPESVANQIAAGEVVQRPASVVKELLENAVDSGASSI
                     DLFVKDSGKTLIQVVDNGRGMSVADARMAFERHATSKICRAEDLFAIHSFGFRGEALA
                     SIASVARVEMKTRDEVSEAGISIVIEGSKFISQEICAAPKGTNIQVKDLFFNIPARRQ
                     FLKSNNAEMHHIWDEIHRVAIPNHHIRFRIFEGSRILSTHEPSNLQQRIVSLFGKQYQ
                     QRLMSVSQDTQYVRVSGVVIRPEFSKKTRGEQFLFVNNRFIRSNYINAAIKNAYQGLI
                     PADAFPGYFIFIEVDPAEIDINIHPTKTEIKFRDERMIATMITSTIRRSLGTGNAMPS
                     IDFEVEQGIDFSLNPDREVKIPQIKINPEFNPFRNSVQPIDYTSAERSRLHWENDTIP
                     SPDPLPEDTLPDQFVPQNETFNTESYIQAGRRFIISNVKSGLLLVDQHQASLRIVFDQ
                     LVNSGHHESKNARLLMFPENISFGAADSELMAAISDELNNCGFVISQLGRDMFSVSAV
                     PDDVELNGISVKQLLEGVLEDYKSAGALRTDRREAVILSMARKLSVRHGQFLNSEMMK
                     LMISKLFASSNPEISPEGKNIFTIVSADDLAKWLH"
     gene            48896..49720
                     /locus_tag="PKY63_00870"
     CDS             48896..49720
                     /locus_tag="PKY63_00870"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPE99192.1"
                     /translation="MNDYYRPGGFSMMPPAVKNLLIINVLFFVATWILRDKGIDLYQI
                     LGLSWVQSENFRPWQFISYMFMHADFTHLFFNMFALWMFGYLIENFWGAKRFLTFYFV
                     TGIGAALVQLGINWFEFSHLHNVLEIYASNPNITDFKEIVQQHFASRENVDAANNLLR
                     NWSMRPDDTLFIGQSMEQLRLMMEGRMAIPMVGASGAVFGILLAFGMMFPNQRIYIYF
                     LFPIKAKYFVIIYGAIEFFFGISGAQSGVAHFAHLGGMLFGFLLIMYWRNKHKFRR"
     gene            49723..50640
                     /locus_tag="PKY63_00875"
     CDS             49723..50640
                     /locus_tag="PKY63_00875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPE99193.1"
                     /translation="MFYHHPQQGGFLNRFFSFMFSKQAVSRLILFNTIVFILVLLNGL
                     NDFLFKLKNVYPDALSALAYYLALPADINQLAARPWTAFTAMFLHSSFWHFLLNMLML
                     YFSGVIFLSVLNNRKLWISYFIGGLSGAVAFVAAYNFFPVFENIIPISYAIGASAAVM
                     AVLFTVSAYAPNMSLRLFLFGQIQLKYLALIFVLIDVLSITASNPGGHIAHLGGALFG
                     VIYGLWLKSSSVGGFSMNARLKKMFKRKPKMKARVNNHNRPESDDEYRNRKAADNKRV
                     DAILDKISKGGYDSLTKEEKDFLFRHGRK"
     gene            50730..51929
                     /locus_tag="PKY63_00880"
     CDS             50730..51929
                     /locus_tag="PKY63_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HPE99194.1"
                     /translation="MGRVLIIGAGGVGTVVAHKCAQLPEVFSEIMLASRTKSKCDAIA
                     AKIGGNRIQTAAVDADNVEELTKLLKEYKPQLVINVALPYQDLHIMDACLNAGVNYLD
                     TANYEPIDEAKFEYSWQWAYQDRFKEAGLTAILGCGFDPGVTSIYTAFAKKHYFDEMH
                     YLDIVDCNAGDHGKYFATNFNPEINIREITQDGLYWENGKWIETKPLEIHKPIEYPNI
                     GPRESYVMHHEELESLVKNYPHFKRARFWMTFGQRYITALEVLQEVGLTSIKPIKYQG
                     VDIVPLQFLKALLPDPGSLGENYKGETSIGCQIRGIKDGKERTYYVWNNCSHEQAYKE
                     TGAQGVSYTTGVPAMIGAKMFLEGKWSGAGVFNVEEFDPDPFMADLNKYGLPWQEEID
                     KPLPVEK"
     gene            52058..52420
                     /locus_tag="PKY63_00885"
     CDS             52058..52420
                     /locus_tag="PKY63_00885"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99195.1"
                     /translation="MNIEKFEDIIAWQRARELTKLVNCNFGSSRDSNFRDQICSASLS
                     IMNNIAEGFERKSNNELRQFLFYSKGSCGEVRSMLYVAFDLLYISKERFLLLMELCLE
                     TSRLLSGFIKSLNTTNKV"
     gene            52483..53613
                     /gene="nspC"
                     /locus_tag="PKY63_00890"
     CDS             52483..53613
                     /gene="nspC"
                     /locus_tag="PKY63_00890"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPE99196.1"
                     /translation="MHNSIPSPCFVIEEKKLLANLQLMQKVREEAGVDILVALKGMSF
                     WHYFPLMKQYLSGACASSVNEARLIFEEMGVKAHTYAPAYEENDFEQLLQLSQTITFN
                     SLYQWNKYKDKMLQHPEIHAGLRINPGYSEIETDIYNPAVPGSRLGIPLEQLPATLPE
                     GISGLHFHVMCEQDSYVLERVLKVVEEKFGHLLKQAQWFNMGGGHHITRADYKPEHLI
                     ALLKDFKSRYPNLKIILEPGEAVGWRTGFLLATVLDIVESGGIKTAILDVSFSAHMPD
                     TLEMPYKPIIVGAGEPNEFSFNYRMGGSTCLAGDFYGDYSFPNELKPGDRIEFEDMIH
                     YTMVKTTLFNGVRHPHIGKIDCDGKFTLLRSVGYEEYKKRLS"
     gene            complement(53853..54371)
                     /locus_tag="PKY63_00895"
     CDS             complement(53853..54371)
                     /locus_tag="PKY63_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99197.1"
                     /translation="MKKSIVFYLLIAGLLISIIVFLLCILHKKTNQIELLELKIEADS
                     IKYSRQISENNNAFNFKQIAVNAFSENPTVVVGDSLHAQVNISAFDMIKNGNHMGIPY
                     LVVGNALDTINLKMIGKCDTIFQNSWEAKLSLKTGKIGPNTIVGLYCIPDTISNETLH
                     FPFRLKFNVIDK"
     gene            complement(54531..55898)
                     /locus_tag="PKY63_00900"
     CDS             complement(54531..55898)
                     /locus_tag="PKY63_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE99198.1"
                     /translation="MRNQRLLLLSIIISSVLSPFLSAAMNVALPQLSRDLGMNAAELS
                     WVNMSFLLASAAFCIPLGKMADRIGRRKMFIAGNYLVMLTSVACIFAQETFFFLALRF
                     IQGIGSSMLFVTGTAIITSAFPKEKRGKFLGYNVTAVYIGLSAAPVLGGFLIEFINWQ
                     SLFYVSIVNSALVIGLLMFYSGEDWKTGRNTPFDWIGSIIYVVFMSMLMYGFANIIQP
                     EAQLLTAVGAAGIIAFIIYEYRILHPVFDVKFFVSSRRYAFSNMAALINYAATFAVGF
                     LLSLYFQYTRGLSPAATGAILMIQPVVMAISATISGRMSDKYDSRILASLGMLLSAIG
                     VGMLVFATAETPHEFFYAALAILGLGFGFFSTPNTHSVMNSVEPPQFGTASATLSTMR
                     ISGQMISMGMAALFISWLIGAHRVNAANALRLVDVMQYVFGIFFFLLLLGTWFSWARG
                     KHTAI"
     gene            complement(55962..56234)
                     /locus_tag="PKY63_00905"
     CDS             complement(55962..56234)
                     /locus_tag="PKY63_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE99199.1"
                     /translation="MNKAELVDAIAAEAKLTKVDSKKAIEAFIKVVKGSLKKKNNVVL
                     VGFGTFKVKKMAARNGKNPRTGKTIKIAAKNVVRFKAGTELAKKVK"
     gene            complement(56795..57067)
                     /locus_tag="PKY63_00910"
     CDS             complement(56795..57067)
                     /locus_tag="PKY63_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE99200.1"
                     /translation="MNKAELIDAIAAASKMTKADSKKALDAFMTVTGKALKKGDRISL
                     VGFGSFSVAKRSAREGVNPRTGKKIKIAAKKVVKFKAGAALATSVK"
     gene            complement(57243..58199)
                     /gene="fmt"
                     /locus_tag="PKY63_00915"
     CDS             complement(57243..58199)
                     /gene="fmt"
                     /locus_tag="PKY63_00915"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPE99201.1"
                     /translation="MLKIVYMGTPDFAVGPLEAIFNAGHEIAAVVTAPDKPAGRGRKL
                     TASEVKKFADAHDLPLLQPVNLKDSAFLNIIKEINPDLIVVIAFRMLPEAVWRIPSKG
                     TINLHASLLPQYRGAAPINWALINGETQTGLTTFFINETIDTGDIIMQEAVEISENDN
                     FGTLHDRLKEMGKSIILKTLDTVESGTAKAIAQNNIQKDSKPAPKLNKENTRIDWHQA
                     TKDIFNKIRGLSPFPGAWTMLMSENGTEQLLKISDAGSGMTETSSQSPGTIISDGKTY
                     IHVVCGQGMLQVNKLQLSGRSMMTTAEFLRGYAGLLNGAILK"
     gene            complement(58212..58640)
                     /gene="ribH"
                     /locus_tag="PKY63_00920"
     CDS             complement(58212..58640)
                     /gene="ribH"
                     /locus_tag="PKY63_00920"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPE99202.1"
                     /translation="MRFAIVVSDWNSEVTNALLSGANGLLLQCGAEESNIQTIHVPGS
                     FELCVAAEWMARKNCFDAIICLGCVIQGETRHFDFISQAVASGIAQVGINHAIPVIFG
                     VLTTNNMEQALARAGGKHGNKGVEAAATAILMVSQKKLLR"
     gene            complement(58705..59403)
                     /locus_tag="PKY63_00925"
     CDS             complement(58705..59403)
                     /locus_tag="PKY63_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99203.1"
                     /translation="MAKQTTPSGDERLENIGSTLSKAEQFIEKNKNIITYVIAGIALV
                     VLGIWGYNNFIHGPKVTKAENEIYMAQMYYEMDSLNLALNGDGNHAGFLEIIDNFGGT
                     PSGNLAHYYVGMIYLKQGLFEDAIEELEKFDGDDDIIGPMATGCIGDAYMELGNQEEA
                     LEYYLDASEQSENSFTSPMYLMRAAWVYEAMGDWDNALELYTKIRNDYRKSFEGSEIN
                     KYIARATAMKNSAQ"
     gene            complement(59510..62074)
                     /locus_tag="PKY63_00930"
     CDS             complement(59510..62074)
                     /locus_tag="PKY63_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPE99204.1"
                     /translation="MEAKFSQRVMDVLGFSKEEALRLNSPYVGVEHLFLGMLREGDGM
                     AIHLLRNAGVNLGEIRKSIEKAANQTDGPAIMPETDIPLVRQVEQILKLTYLVAKDLK
                     SELIETEHLLLAILKENKNYVSTLLGRTGATYDLMLSELRNITGNDTHYSERIKDELP
                     SDDDDADERSSFGSSSKKTGAESKSKTPVLDNFGRDLTKAAEEGRLDPIVGRDKELER
                     IAQILSRRKKNNPVLIGEPGVGKSAIAEGLALRIAARKVPRTLHNKRVVMLDIASLVA
                     GTKYRGQFEERMKALLNELEKNRDVILFIDEIHTIVGAGGASGSLDASNMFKPALARG
                     EIQCIGATTLDEYRNYIEKDGALERRFQKILVDATSIEETKIILQNIKERYEDHHLVR
                     YTDDAIEACVHLTERYISDRALPDKSIDALDEAGSRVHLKHLNVPQEILDLEKQIDEA
                     RNNKTAAIKMQKYEEAAKHRDQEKNLLDQLEKARLRWDEEADKHREVVTDEDVAEVVA
                     MMTGVPVTRVAQNESDRLLKIENELGDKVIGQNEAIKKIAKSIRRNRVGLKDPNRPIG
                     SFIFLGPTGVGKTYLAKVLSKYLFDTDDALIRIDMSEYMEKFAVSRLIGAPPGYVGYE
                     EGGQLTEKVRRKPFSVVLLDEIEKAHPDVFHLLLQVLDDGQLTDSLGRKVDFKNTIII
                     MTSNIGTRQLKEFGSGVGFATSSKESSKAGYAKSVLENALKKAFAPEFLNRIDDVVIF
                     NNLERNEIHKIIDIELSGVLKRLQEKGILLKLTDEARDFIVEKGWDPNYGARPLKRAI
                     QKYIEDPLAEELIKHNFEAPTDIIMDYFNENDELTITSSSATKNKKNEADQKNLTE"
     gene            62356..64905
                     /gene="gyrA"
                     /locus_tag="PKY63_00935"
     CDS             62356..64905
                     /gene="gyrA"
                     /locus_tag="PKY63_00935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPE99205.1"
                     /translation="MSEFENSGERIVQVNIEDQMKSAYIDYSMSVIVSRALPDVRDGM
                     KPVHRRVLFGMHELGVFSNRPTKKSARIVGEVLGKYHPHGDTSVYDTMVRMAQEWSLR
                     YPLVEGQGNFGSIDGDSAAAMRYTEARLRKIAEEMLADIEKETVDMQLNFDDSLEEPT
                     VLPAKVPNLLLNGASGIAVGMATNMAPHNLKEVVDGIVAYIDDNEITIEGLMKFIKAP
                     DFPTGGIIYGYDGVKEAYETGRGRIVMRGEANVEDGDKHTQIIIKSIPYQVNKSELLK
                     KFADLVNEKVIDGVTDLRDESDRDGMRIVLDLRRDAVPNVIINQLYQHSQLQSAFNVN
                     NIALVHGRPMMLNLKDLIKHFVDHRHEVVVRRTQFELREAEKRAHILEGLLIALDNID
                     EVVEILRSSKSVDEARERLMERFSLSDIQSRAIVDMRLRSLIGLEREKLRAEYEELMK
                     RIAWLKSVLEDFALRMQIIKDELLEIKEKFGDEPRTDIVYQGKNFRIEDTIANDSVVI
                     TISRMGYIKRTLLSEYRTQNRGGKGLKASNVRDEDILENLYIASNHDYLLAFTKKGKV
                     FWLRVYEIPEGARASKGRPIQNLLAIENDDKIQTVINVADLTNKEYVENNFIILCTEH
                     GVIKKTSLEAYSRPRQNGINAIGIREGDTLIQASLTNGKNEVLIALKSGRAIRFPEEK
                     VRPMGRNASGVRGVTLAGADDKVVGMVCIQDKLEQILVVSEKGFGKRSDIEDYRVTNR
                     GGKGVKTINVTEKTGKLVAAMSVKDGDELMIINVSGIAIRMSVSDLRVVGRATQGVKL
                     ISLSGKDSIASVAKIASDFVEIEEIVEENVEENGSDLAGNESSETPDETKE"
     gene            64975..66171
                     /locus_tag="PKY63_00940"
     CDS             64975..66171
                     /locus_tag="PKY63_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99206.1"
                     /translation="MKKLALFVVALVFSGVAFSQNPAVVSAFNWLKDNKLDKALAAIE
                     PATTHPQTMSSAKTWLYRGNIYLNIALTDNESFKALCSNPLDSAYNSYQKSIMLDKDY
                     IAPTANPNSAKLGLFYLGDQYFNVGANHYNNKDWAEARKYFERTKQIKNTFGEKDSLA
                     TFYATNCALQMGDNETAMKYLKELINMDYQKPEIYSMLGNLYKDAGDTTKMLNTIAMG
                     RKRFPADLGLIISETNYYLGKGELAKAQDLLKVAVEKDPNNPILHFTIGSNYDRLSLD
                     TNVAEAEKESMLAEAEKAYLKAIELKADFFDAYYNLGALFFNKGVEVFELANSIPPDQ
                     FDAYEKAKTKYMEYWNKSVPYLEKASELMPDDLPTLQALKSLYARLNMPEKLKTVTDK
                     INTLKQ"
     gene            complement(66387..>66570)
                     /locus_tag="PKY63_00945"
     CDS             complement(66387..>66570)
                     /locus_tag="PKY63_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99207.1"
                     /translation="KVKVKVKVKVKVKVKVEVKVKVVVKVEWLPVCRSGSEISSQFQA
                     WPSDRFNTKEKIVVHV"
BASE COUNT        18535 a        13665 c        14714 g        19556 t
ORIGIN      
        1 tttgatgaat atgtttctaa ttttgtgagt gttaagtgtt tttcagcagg ccctaggtaa
       61 tgattctgat tgataaaatt gggtcaatat agtttgtaac tattgtttag actggcttgt
      121 aaaataccgg tttgttttaa gtctcataaa taatgggttt tgtttaagta atatgtttgt
      181 acatgagtat tattgaacat tttgtgaaaa tgatttttgt aaaagctgta tcaatcacat
      241 tatcaggagg tgttttttat ctatgcgata tttgcagaac tggatataaa atgtcaaatg
      301 gaatgacaat cattgatgtt tatttgtttt tttcaatgaa tgaaatggca gtaatcaaca
      361 tcgtggataa ggatgttgtt ttacataaaa gtatgatttt ggaatcaata cagcaatgtt
      421 acctttcccc tgtacttatg aatgatattt gaagcatctt taataaagat ttcataagtg
      481 taggtgtcct gtaatttttc ttttacgatt ttgccgttga tgtttccatc ccagggaata
      541 ttggcttgtg ttgtattgaa caccagatta ccccaccggt tgtagatgcg catttcgtaa
      601 taatccagat caactgagat gccatagggc ataaagaaat cgttgtgtcc gtcaccattg
      661 ggcgaaaagg cgttggggat gtagaaagca aacaggtcat taatgataat ggtgagttga
      721 aatgaatcgc ggcagccaaa attatcggta gcaagcaatg taacgagata ttcgccaact
      781 ccgccataca gatgcgaggg gtattgttca accgatgtgg ttccatcgcc gaaactccat
      841 tcatagtttg atgcaccgga gcttgagttg atgaattgaa tcggccctcc accaatggtt
      901 gcaggatttg ggttggcata gaaagatgca ttggggccgg gcgccagtgt tacatatgcc
      961 acattggaat aagtccctat tgaatcggtc attgttactg tatatgcgcc aggggtgagg
     1021 ttgctgattg tttcgctcgt ttgtgcaggg gttgtgttcc acgaaatggt gtaacttcca
     1081 acattattgt gaaccacaac ggtggctgtt ccatcggcat gtccgcaatg ctctggtgtt
     1141 gttgaaactg tcagatatgt attggttgaa agaccaatga aaacaatgag tgtatctgaa
     1201 catccgctgg catctgttgc tattacggtg taattaccgg tggatagtcc gctggcgctg
     1261 gttccagtct gcaccggcga tgtgtcccat gttattgtta ttggcgctgt tccgcccgaa
     1321 gtactaacaa cggctgtccc gtcctgcatt ccgtaatttt cgggggttga acttaccagt
     1381 gacagagcgg gccctgctac gttcccaacg gtatatgaag ctacgcttgg acacagacct
     1441 ccgtcatcca cagttactga atatgttcct gtaataagtc cgctgatatt tgaagttgta
     1501 gcaccattgc tccaaaaata ggtatatgtt cctgatccgc ccgatgctga aacgctggct
     1561 gtgccgagcc cctgattgca ataatcggtt gttccgttta ttgttagttg agggcctcca
     1621 ttatcaatga ttgttgctgt atctatggct gagcatgcac cgtccgaaac ggttacgtaa
     1681 taaattccgg cagcgaggcc tgtggctgtg gcagttgttt gtgcaggtgt ggtacttcaa
     1741 agataagtat aggttcctga tcctccgtat ggcgttactg tcgcactgcc gttggggccg
     1801 cagttggcat cgactgtagt gatattggtt gaaacgcctg aaactacaaa gctgaaacat
     1861 cccagagccg cagcattccc acaggcgtcg gtaacagtac tcaccatgca gatctgataa
     1921 tttccgctgg tggtcattgc aggagacact gtaagggtaa aagtgttgtc catgtctgct
     1981 cccgatgaac aagcagcact tgaaatagaa acagaagtgt aagttgcacc gggtccggtc
     2041 acttgaaaat ctgaagcaag aacagatgaa cacattacat tctcactgaa attaaaagtg
     2101 atttgtgttg ctccgcaggc aacaggcaga ttgactgatt gaaaaaccgg aggactatta
     2161 tcgaatatcg atgcagatga cgggccaaag tcaagtgtgt atccatattg agttgatgaa
     2221 taattggaaa tattcaaagc gtatgtttga cccgcagtaa ccggaataac agcgttgtca
     2281 ttaccatcca ttgcatctgc acagttgagc gatgagccgc cgtttgctcc ggttgtgcca
     2341 ggggtggcag agtagttaca acttacctgc aaagatgggt ttgaatagat atcatcgcag
     2401 ttggcattgg taagattata cacagcccag tcgtaatcgt cagaactact gtttggcgta
     2461 atattaaagt tcagatttcc tgaagtttga acagtgaaaa tataccagac atcatttttt
     2521 tctcctgata gcagacaact gccaggacag ccactggtgc tgatttcgtc cgtatagttt
     2581 ccagttcctg aatatgaatt actttgtgta taggtgtatt gacatactgg aatagccccc
     2641 agacaatcct ggttggtagg agtctgcgat agaacaaaca aaggcaagag aagaaaaaag
     2701 atggaaaata ttttattcat agccccctga ttattaattt ttgttcttca acattttcat
     2761 tctgaaccct tcaaccggat acggttcatt atcgacaaaa accgtgtaaa ttccggcaac
     2821 aacaaacagt ttcctatgct ctttaatttt tactgctggt tttgtaattt catgaatttc
     2881 ccaaacattg ggttcgcccg ttggtttgat ttcaaattcc tcaaacatgt tgtataaaag
     2941 atataatcgc ttcaatgatt caatttcggc ttccgtttgc ggtccttcaa ttttgtaact
     3001 catcaccagc cggtctttgt tgtcgcgata cacagtctgc tcagtattat cgaaaaacgc
     3061 aatgtaattc tgcgattgac caaaaaggtt ggacccacaa aacactataa gcagaacagc
     3121 cagcgatttc agtattgttt tcataacaga tgttttaacc cccaaagcaa ttgatttgta
     3181 cgtaaaaaaa taatctttgt ttagacgaac attttacagc taagttaaat atctatttta
     3241 taataagtaa attattttca caaaatcatg cgtttgtgaa gacgaaattt cttaaatcgc
     3301 agatatacag tgttgtagct atttgctttg gttggtcaga ctggtgtaat gttttttctt
     3361 tctgaagtaa taatcagata aaatattgcc tgcataaata gttgccggaa atgaaccggt
     3421 ggtggattta acaaattgct ttttcattga cgaaaaatga cgccgcgcct tgattaccgc
     3481 ccaggcatcg gcaaaatgtc cctgtagaat gaacattccg agcgcaataa aatcaaggaa
     3541 aaagcggcgg cgtaaaactt tgctcagttg gctggccgga taatttttat aaatcagcaa
     3601 cagattgttg cggaaattca gataagtctt tcgcgccgaa gagcggggca gactaccgcc
     3661 accaacatgc caggcctgac tttgcggaac taccgctatt ttgcggccgg cattccacaa
     3721 acgccagcag aggtcaattt cttccatgtg ggcaaagaat ttttcatcca gtccgccaat
     3781 ggcgtcatag tcgcttttgc ggatgaataa acaggcgccg gtagcccaca gcacatcaat
     3841 aatgtcgtcg tattgtcctt tgtcttcttc aagggtcgaa aaaatgcggc cgcggcaaaa
     3901 tggataagcg tttttgtcga gaaatccgcc gctggcaccg gcatattcaa acatgttgtc
     3961 atgtccgtac catttcagct tgggctggca ggcagcatat tcggaatgcg cgtccataaa
     4021 ggattccagt ggttccaccc atccgggttt tgtttcaaca tcggaattga gtagaacaaa
     4081 atattctgca tcaatcaact tgaatgcttc gttgtatccg ccggcaaagc cgtgattcac
     4141 ttcgaattca atcaggcgaa tgctgctgaa tttttcttta agcagttcca gactgccgtc
     4201 ggtagaggcg ttatctgcga caattaattc ggctgttgct ggcaacgatg caataacgcc
     4261 gggcataaat ttctcaagcc agccctttcc gttccagttt aatataacta ctgctgtttt
     4321 tataacgttt tattcgatgc acaaatgtac aattttttgc acaacgcaca acgcctgatg
     4381 cctgacgcat atcgcctgat actttacgtc tcaccgattt atcacgtttc acgtgaaggt
     4441 gcttaacctc aacctcaacc tcaacctcaa ccttaacctc aaccttaacc tcaaccttaa
     4501 ccttaacctc aaccttaacc tcaacctcaa cctcaacctc aacctcaacc tcaatcttaa
     4561 cctaaccatt aaccattttt attatctttg aaaaaaaaac agcatggccg aactcaagac
     4621 caaacctgaa aatgtggatg tgcagtcatt cattgatgag cttcccgatg aacagcgtga
     4681 agacagtaac tatctgctta agacgatgca gaaaatctca ggtgaaaaac cagaattctg
     4741 gaccggtggt atgattggat tcggccgtta tacctatcag ggaaaaagct gcagcggcga
     4801 atggttcccg cttggttttt caccccgcaa aaattatctg agtatttatc tgctgggtta
     4861 tattgacgaa agtgatcctt tgttgggaaa actggggaaa tttaaagcag gcaaaggttg
     4921 tctgaatatt aagagacttt ccgacattga taagaatgtt ctggaacaaa tcatcaaaaa
     4981 caataaaaag aaatagctga atagtgaatc ccggagacat tttacaggtt aacctgaaaa
     5041 aacacataga actttccgaa accgaacaat actgggttgc tgaaacagtt gacggacagc
     5101 ggtttctgat tgattatttc cagtatgtgc attatggttt tactaccggg catagcattc
     5161 aggtgcaggt tgataaaatt aattgcagcg gaaaagtttt tgtagaacct aatcatccgg
     5221 tgtatgaacg ggaaaagact tacgtatttt caattgattc tgtgttatca aaaaacgaaa
     5281 actatttttc attgattgta aaagatgttt tcgacaataa tatctcggtt cagtttcatg
     5341 caaaggggtt ggatagaatt cccgaatcgg ttgaattgct ggtcattggt ttaagaaaag
     5401 gattgcccgt tttacttgat ccaatgctga gagaacaaac accgtttaaa gaaaatgaag
     5461 tctattcttt tatggttgtt gataaaatca aaatgaatca tgaagaatat tttcaactcg
     5521 aagatgagtt tcaccggatt catttattac cagcaaggtt ttacgaaaag tatccgattg
     5581 tgattgggaa acctctccaa tgctacgttg tacgcatcga ccaaaatgca gagctgaagc
     5641 tcgaaccggt gcatccattg tttcatctgg gacatgaact cgatttgtat tttgtaggtg
     5701 aataccatgg cgatgaatat attggtcaac gacacaaatt atatttgttg catgatgatg
     5761 aaggcaatga attttttatg tcggtgcgtt ttatggaagg ccgcgaaatt ccaaaacgtc
     5821 tgcgttgccg aatcgaaaaa ctcaaaaaag gacaaccgtt ggttgaacct gtagatgtgt
     5881 aacagtgcgg taacttaaaa tattattaac gtgcaggtaa tattgaaaat tcaggggtaa
     5941 gtgtatttaa tgtacctttg ttttgaaata ttaaataata aaatgatatg agtaaaaaga
     6001 aaaccaacaa cacaaacaaa aatcaggaag acaacattat tgccatgaat attctggcca
     6061 ttttccgtca gaatccatac atgctgttca actataaaca aattgccgga agactcggaa
     6121 tcaatcgtgg ccccgaacgt gagaaaatgt gcgcagccat tgaccgtttg gtttcacagg
     6181 agctgcttct ggtgcggaat cgcggcaagt atgttctcaa tcccgaggcc atcgaagtgg
     6241 atgaaaatgc cagcgaaatt gaaggtgtta ttgatattac tgccaacggc aaaggttttt
     6301 tgctgatgcc ggataaagac gatattgcca ttgctcaggg cgatttgaat caggcactca
     6361 ccggagatac cgttctggtg cggcttttcc cgcgaagaaa aaatcgccgt cctgaaggca
     6421 aagtcattcg tgttgtaaaa cgcaaacgtc agcaatatgt tggaatcatt caggttggag
     6481 caaagatgtc tttcttcgtt cccgatgatc gcaaaattca tattgacttt cttgttccgc
     6541 gcgaaaatct gaataatgca aaaaacggcg aaaaagtaat tgtggaaatt actgattggc
     6601 cgccgcaatc acgcaaccct tttgccaaca tcgttacggt gctgggacag cccggtgtga
     6661 atgaagttga gattcagagt atccttgcga actatgaatt tccattgtgt ttcccaaaac
     6721 atgttgagaa agaagctgaa cgtattccgg tcgaaatcag tgccgaagaa atcaaaaaac
     6781 gcaaggattt tcgcactact tttacgttta catgcgatcc ggccgatgca aaagatttcg
     6841 atgatgcctt gtcgctgaaa aaactcagta atggtaattg ggaagttggg gtgcacattg
     6901 ccgatgtttc gcactatgtg aaaaccggct ctgaaattga taaagaagca tttgaccggg
     6961 caacttccat ttatatggtc gatcgagtta ttccaatgtt gccggaggta ttatcaaata
     7021 atgtgtgttc gctgcggcca aacgaagaca aactttgtca ttcggtggtg ttcgaaatga
     7081 atgaagaagc gaaagtcctg aaatactgga tcgggcacac ggtcatcaat tccgatattc
     7141 gctacaacta tgaggaggtg caggaaatta ttgaaaccgg caaaggaaaa tacagcgagg
     7201 aaattttgtt gctgcacaag ctggcgacca tcatgcgcga agaacgcaat aaaaaaggca
     7261 gcatcaattt taaaagcacc gaagtaaaat tcaaactcga tgatgacgga aaaccgcttg
     7321 aggtttacgt taaagagcag aaagaatcga acatgctgat tgaggatttc atgctgctgg
     7381 ccaataaaac agtggcagcg cacatcggta tgaaaaccga gaaaaataaa aatccaaaaa
     7441 cctttgtata tcgtattcac gatgaaccaa gtcccgaaaa acttgcgaat ttttctgaat
     7501 tcctgggcaa acttggatac agcatcaaca ttacttcgaa gcagaatatc tcgtactcgc
     7561 tgaataatct gttcaacaag gtgtcgggca aagctgaaga gacaatgatt gaaaccattg
     7621 cggtgcgcac catggcaaaa gccatttaca gcacgaacaa catcggacat tacggactcg
     7681 ctttcgatta ttatacgcat ttcacttcgc cgattcgccg ttatcctgat ctcatggtgc
     7741 atcgtctgct cgattggtat gccgccagca aaccttcggt gaatgaagcc gaatacgaag
     7801 atatgtgcga tcattgcagc gacatggaaa agaaagctgc cgacgccgag cgcgaatcgg
     7861 tcaagttcaa gcaaatggaa tttatggccg atcaggtagg aaaaacattt gacggtgtaa
     7921 tttccggcgt gagcaaatgg ggcatctttg tcgaaatttc atacagccac gccgaaggtc
     7981 tggtgcggct cgaggatatg aaagacgatt tctactacct cgacgaagac aactacatgg
     8041 tcatcggaca ccgcaccaaa accacttatc gcatcggcga caaagtaaat gtgctggtca
     8101 aaaaaatcga tatcggccgc aagcaaatgg atctggcgat tgtgtagtac gctcagagct
     8161 caacgcataa cgcacagggc atggcttctt tttcaaattc tctgcgatgc gaagcgatct
     8221 gtcacgatta ctgtcgtgag cgttgacata accctgtatc cctgttaccc tattaccttg
     8281 tcaccctgtt accttgtcac gattgtaacg actgtcacgc gtaacgacat attcaaacaa
     8341 acagacaaaa aatatcaaaa acaattagta ttttcgcata ttaaatattc agaaaaatga
     8401 gagaatataa aatcttccac ggtaaaacac tgggaattct ttccggcggt ggtgatactc
     8461 ctgccataaa ttcaagcatc gaaaacattc gtaatcgtgc acagctactt ggttataaag
     8521 tatttggtat tcgtcatgga tggaaaggtt tgttgggtga tggtgatgtg gtcgatctga
     8581 ccaatcagcc ctatgatgga atttatggag gaactgcact gcgctcaagt cgcaccaatc
     8641 cttttccttc aaagaaaaat ccggaaagcc gtgttcctca gattctgaaa aacattgaac
     8701 gctataaact ggatgtgctc gttaccatcg gtggtgatga cacaaacggt gcggccaaac
     8761 gtctctacga aaccgaagga attccggtta ttggttttcc gaaaacgatt gacaacgatt
     8821 tgcgtacgcg caccgtacat gaatattcca ataaaaaaat cgaagcggta ctttgtcccg
     8881 gctatcccag cgcgctcaaa ggtgtgatgg aatttacccg tcgaattaaa accacagccg
     8941 aatcacattc ccgcattctt gtactcgaaa tcatgggtcg tgatgccgga tggcttacag
     9001 gcggcgcagc atatggtcag gccgacatca ttcttgttcc ggaatatgct atggataaaa
     9061 gccgcaagga tttctttttc gaatttgtga aggaagcttt tatgcgtagt ccgaaaaaat
     9121 cgcttattat tgctgtttca gaaggtttgc gctggtacga cgaccagtcc ggtaaaaccg
     9181 atgtggtgta tgcttctacc gaagtcgatg aatacggaca cccaagactt ggcggtgtga
     9241 gcggcgtgat tgccagcgaa atatcaaata aacttggcat cgaagcccgt gcacagactg
     9301 tcggatatta tgcccgctcg ggcgaatgtc gcttatatga ccgcaagctg accgcaactc
     9361 tggccgacaa ggtggttgat ttactgctac gcgaagatta tggcaaaatg ccggttatca
     9421 gcaaaatggt cagttatccc gaactcgagg agtttaatac aggttgcatc gatatgggga
     9481 atgtgggaaa taaaccactg cctgaagaat attatgatgt ggatgaattc cgctttaccg
     9541 atgcttacta cgatttcatg caccatattc ttggtgaccg cacatttatt gatttcaaat
     9601 acaatttccc ggtcgttatt cctgaataat gaagattttt ttctggattc ttttatttcc
     9661 tgtcgtattg caggcgcaga cattgtcagt cagcaccgac agtttgatga agcatgtgaa
     9721 ttattttgct tctcccgcac tgaatggtcg tttgccgggc accgatggat ttctgaaagc
     9781 tgttcagtat tctgaatatt attttgaaca gcatgagctg acgcaggttt tgaatgattc
     9841 ctggcagcag aatattccga ttgaaacgaa tgtgattgat tccgcccgtg taatgataga
     9901 aacaggtcgc gatatgctgg agctgattcc gggagtcgat ttttcggtcc gcggatattc
     9961 cggcagcggc aatgtgactg caccggttgt attctgtggc tacgggtttg attcagaaaa
    10021 atactcggat tacaaagata tttctgacaa agatttagat gtcaaaggtt gcattgtgat
    10081 ggttttcaaa tctaatcctc cttttattga aagccttcct gattattcaa tccgaaaagt
    10141 tgctgacatt gcatataagc atggagcagt tggagtaatt tttgtctcac cgccaaatca
    10201 gaaaaacccg caaaagccta tcggcagcat gatgcatggt cctggtgcga tgcacgcgga
    10261 tttacctcaa attcaaatgt cggttgagaa agcagactat gttgctggat ggcattcttt
    10321 actcgaattg caaaagagga ttgatacgct tcaaaagcca ttaagtatgg gctgtcaaag
    10381 acatgtcaac attcaagtga atgctactta caatgcaaac gggaatacaa ccaacataat
    10441 tggaatgtta cctggctccg atccggtgct gaaagatgaa tatattattc tgggtgcaca
    10501 tctcgatcat gttggtgcaa tcggtaatac agtgtattat ccgggtgcaa acgacaatgc
    10561 ttctggcagt gcggctgtac tggaacttgc ccggattttt tcggcagaca gaagcaaact
    10621 gaaacggtca gttatttttg ttctctttgg cagcgaagaa aaagggctcg atggctcgac
    10681 ctgttttgta aataatctgc cggtgccgaa agaaaaaatt gtcgctgttt tcaatatgga
    10741 ctgcattggc tgcggagatt cactgatggc aggaaatgga aaaagttgtc cggatctctg
    10801 gaatatggcc attggaattg ctcagcagaa caaatgggca gtgtcgcaac gcacctggga
    10861 tggcggtggc gccgatctga aaccctttta tgatgccgga atcccgggat tgtattttgt
    10921 aaacaccaat gcacaccagc aactgcattt accgtccgac acacctgaaa tcctcaatca
    10981 gccacagttt tcgcgaatgg taatgtgggt tgaggcggtg acgaggaagc tgttgatgta
    11041 aaaaaaggcc tcaaacgagg cctctgaatt tatttctttt tcgagtcgag cggatttttc
    11101 agtccggagt aggagatcag ttcaaatttc accgtgccga tgaggattga gcttttggca
    11161 tagagcggaa tccggttttt gtcgttggta acatagatgg taagcggata agcttcgccg
    11221 aaaacagttc cgacaagtac ttttggtttc agtttgatac agctgaatgt accgagtccc
    11281 attttaagat tggcaattcc gtcataaacc aatgtggttg aaaaaagact atcttcaaag
    11341 acataaggaa tggttatatt ttctccggct ttgaatttgg tcaggtcgag attgcgtgca
    11401 taatagccgg ccgaaatcat atcctgcgca taagttggga tgtaataatt gatggttttt
    11461 tcttttttat tgtctttgaa ttgtgccagg tttttgctgt gattaaaaat aatatcctga
    11521 ttggcggtaa aatttccttc gtgagcgcgg cgcaggaaca gaagcgggaa ttgaccctgt
    11581 tcatcaacat acgaatcgaa gcgatcgttt acataaatgg cccagttgta ttttttaaag
    11641 gtggcacccg atgccacaat gtggttgcat gttcgcccgc caaccgtttc tgtttcaggg
    11701 agaacctcaa aagtgccgta agctgctttg attttgccag tcaaaaatga atcaaaatat
    11761 atgatgtagg ttagtttttc gcctctttta taagcagtgt tttgtacagt gcggtattgt
    11821 gcattagccg caataaaggc gaaaaagaat gcaataaaag ctgcggctga aattaaattc
    11881 ctggttttca tgactgtgag ttttttgtgt ttgaatcctt gtctttatag aacatacttc
    11941 ctacgagcca gcccataatc aatccccatg ccaaagaccc ccacggattg gaggtgatgg
    12001 ggcatgaacc gctgttgcaa ccgactaaat agtaatacaa atagccagca cctgctccca
    12061 ccatcatacc tattatagat ggaagattaa tgatattcct gatgagtttt ttcatatatg
    12121 aacaggtcgt gagcaaacac aaaattatac ttttttacaa tcaatgctga aatttatttc
    12181 agataaggtg tcaatcgttt aataaggact tctttgcttt gcagtcctga tgctttgtca
    12241 actgcaacgc catcgacaaa aatgattgtt gttggaatat actgtatgcc aaagcgttgt
    12301 gaaagagctc tgtttttatc agtatcgact ttgccgattt ttactttgcc atgtaacgaa
    12361 tcggccaggt ggtcgataat gggtccctgc atcacacagg ggcggcacca ggtggcccag
    12421 aaatcaacca gcacaacgcc ttttttaacg gtctcatcaa aattttccgt tgtgattgtc
    12481 agggtatggg tctttttttc ttcggtttgg gcctgagcgt tgttgagtcc tacaaaaaca
    12541 aaggcggcaa cagttccggc aagaataaga agtttgtttt tcatgcgtgt ttttttgcgg
    12601 ggcgcgattt tcaaattttg taccaaaagt atagcataat ccgtaatgat gattattgca
    12661 gattatgcta tttatcagtt attgaaagaa aatgttcaga atttcacact cacacttcca
    12721 agccagttta tgtaggcctg cgggaagtag ccatccatca cataatgctc cccgccgtaa
    12781 tagtagcgat acacccaggc attcgattca tattcttctg aaaaaatatt acgcagcgtc
    12841 agacggaaag cgacttcttt gacaaatttg ggtttcagca catattctgc tccaaggtcg
    12901 aaaatcttgt atggatccag cgatcgttca atcgaagatg aattgtcgat gtactgcctg
    12961 cttacatatt ttccgcttag tgtaaaacgt agatttttaa tcgggttgta gccgaaacgt
    13021 gcagatccga ttatagctgg cgaaaagctg atgtcgcttg ttttgtaaaa ctcagtgtgc
    13081 tgcgaccagg tgtcccagtc atcaataaag actagtagat cttttatttt attgacagag
    13141 taagttgcat ttcctgaaaa atcgaatttc tcattgactt tccattccca gataccttcg
    13201 atgccagcgc ggtagctttc aggtacattt acataaatgg ctgaacctat attgtttatt
    13261 tcaccggttt gtacaagctg attgcgatag ttcatataaa acagattcag attgatattc
    13321 cacttatcgg ccgtgtattt gtatccggct tcgtaatcaa aaagaatttc gtgtaccggc
    13381 atttttccgc tgtcggcatc taccatcatg tatcggtttg gctctcggcc ggcaacacca
    13441 aaatagccat atactgaatg ttttccgaaa gcatgtaaaa tacttgcttt tggattggca
    13501 aacagaaatg atttgtcaat gctcagattt tttttatcat cttcatcgcc ttcaacctga
    13561 tagcgtacat tccggatttg catatcagcg atgattgttg tgtgtttttt tattgaatag
    13621 cgcaattttg cataacccga aatgtcgtct ttaagcccat tgttcaagta gtattcatga
    13681 ttaatatccg agtctccgct atactgcatc cagattacat ttccaatgtg gtcaccatcg
    13741 tagcgactca gatttccgcc cagatttatc tgcagttttt ttcgattatc atagttggct
    13801 gaaaataccg caccataaaa ataattatcc atcatttttc gtcggatcag attggtttca
    13861 gtgattgtat ctccgccaat aatcaggtcg gggagaagat aatcggaaaa tgattcttcg
    13921 tctttatatt gctcatagta tccgtttcct gtaatcagaa aagctgttgc actcgatgtc
    13981 catttctgtg agtgctgatt tgaaattacc agatgaaaat gattttgcgt gtataaatca
    14041 atttcgtttt cataccaata gggattaaat gtcctgttgg tttcgagtga atctttcgga
    14101 acaccttccc aggcctgata ggttttttct tttccgtgca taagcatcat acgcaccagt
    14161 gttttctcgc cataccatgc aacattggtc tgcagcgatt gaatatcggc cgaagcccgg
    14221 tcgatgtagc catcactgtg cattatagat gcgcggccgt cgatggtcca ttgaccattt
    14281 aataaccctg ttccgaaaag tgctgttgaa ccgtaggtgc cgaaagagcc ggctttgaaa
    14341 ttcagtgtgg caaacggatc tggcttatag ctgtgggtag aaatgtttat ggatcctcca
    14401 aaagaagaag agccgtttgt tgatgtacca acacctcgct gaacctgaat gctttcgctc
    14461 gatgaggcaa tatccggaat atcaacccaa tacacattat gcgattcggg atcattgtac
    14521 ggaacgccgt taatggtaac attgatgcgg ctttgatctg ttccgcgcag gcggaaagcc
    14581 gtgtagccaa ttcctgctcc cgcatcgctc gaagtggtta cggatggcaa tgcatcgagc
    14641 atgaccggaa tatcggttcc ggtgttgatt tttgcaatat caacagggtc taaattactg
    14701 caggcagcgg catcctgatt attgatgcgg atggctttga tgacgatttc gtcgctgatg
    14761 actgccgctg gtgtcagcgt tattttcaat gaagattccg gcactgattg taaagaaata
    14821 ctgtcagtat tgtagcccat ataggataca atgacggtga gtgggaaact ggttcgtgtt
    14881 gaaacggaaa aagaaccgtc gctggcgctg aaagtgccgt tgagtgttcc tgaaattaca
    14941 acgttggccc cgggcagtgt tttgcccgtg cttttgtcaa aaactgttcc gctgagtgtt
    15001 gttttttgac tattggccat aaacaacatg gtgaaaaaaa ataaaaagac aactaccttt
    15061 ttcattttgt aacattttaa attacaaatg gccaaggggt ggaaaaccat ttaaaatatt
    15121 gaataaacct ccctgcgccg gcattatccg tatcaggtga taagggtttg ttctcagccc
    15181 ctgctaaagg agcacccctg ttgtgaacgc tgcaaagata cacttttttt aaacgcgcaa
    15241 gacataacgc tcaaaatatg agacatgcgg ctcaattcgt aacgcacaat gcatgaggta
    15301 tcgggataat ctccatcgac aagccgtact gatttgattt tatctggcga aatatttcaa
    15361 agtctgactg atgttgtttc cttttaatgt aatcagatac agaccggcat tttcgggcat
    15421 ataaggtatt tcagtttgtt ccttcaggcc ggaaatgttt tttgtggttg aatacaaaag
    15481 ccggccggtg atatcgcaga gttcgatgga ataagtgccg gccgggaatt gatttacagt
    15541 cagaaccgga aaactgtttt cgtttaccga tatgtacatt gaagaattct gaaaatcatc
    15601 catgcctgta gccactccat gataattgaa tgtaaatcca tcatcggcat tggcttcgtt
    15661 gcttttaaaa acaaccatgg tttttggcga attgataaaa aatgattgtg gaattgcatt
    15721 accagtgagc gaatcaacca gaatataaga aggatactgg taaaaataca gcacgtcgct
    15781 agctggttca agactgaatt ctgtaaaatc aatcattatt ccacctgcac cgggtggttc
    15841 gattctccat cggcagaccg aattgttgtt ataagaataa ttattactgc catcatttac
    15901 ggtgcccgcc tggtcattgc ggatatcaag aatactgcag aatgcaggtg tgtaggtgta
    15961 ataatcagcg tgaaaaccgt catcggtgat tgtgttgttc gattgaaatg taatgaaggc
    16021 acggtttccc ggaacctgta cggaaaaatc gccggaaata ccgctgtaag ttcctgttac
    16081 aggagcagat tcatcagatc cctgataaat ggtaacgaca tcgttgcctg cttctgttct
    16141 taaatatctg aattcgattg taatgtattg aatgctgtcg ctgggctgaa taagccagct
    16201 gcaattctga tcgttactat agtccgaaag cccgtgaccc acttcgagac tgccactgct
    16261 ggcggtcatc tgataattgc cacacgaggc aggataggtt acagcagccg gataacaatt
    16321 gataaaggcc tgttgtccac tggagaaatt atatcccgga ttcagattgc tgattaagaa
    16381 ataaccattg taagagccgc tccaacccca gttgaaatgg aacatatcgt cgctgtagcc
    16441 atcacagaca aaagcatgac ccgagctgac atcgtaaccg gcatatggca taggatgtcc
    16501 tgcattgatt tgggatttca acagggttat ccagtcagta tccgaataat cgtttttata
    16561 ttcaagtgtt gctgaaggat tgtatccgaa atggtccttc attgcattga gcgcatcgtc
    16621 catgtaagca ccagatccgt ttggactgta catcatatca acagcgacac cgcaatcata
    16681 ctgaattttg gcagcatcgt agtttgccga tgtcagacgg tatggcatct gttcgtagtt
    16741 gtaatgactc tgcgaaaaat cgacactcag ttgaccgtaa tccgaaaaat agctgtgata
    16801 gccgcttggt tgaaccgggt atctgtaata atacatggtc atggccatgg ctgtagctac
    16861 gcatcctgca tatacacgat ctccggggcc agccggatcg taggggcaaa gtgcattgta
    16921 aggtacaccc tgatcccatt gacaggtgag cagctgattg acagagtatc ctttatcttt
    16981 ttcaatgatg ccataaatca gttcagtcca ttgttcatta atggaatgat cggctttaat
    17041 gttgttttcg cgaatatgac gtatctgttg ggaataagca ttcaataaag cgacaagtga
    17101 cggaggcata ttttctccgg taaaggaaga ttcagttgaa tagccgagta ttgggctaac
    17161 ggcatcatcg cctgcaataa tgacaaatcc gaactcgttt gcgttgaaaa tataaaattc
    17221 tggattttgg gattgagagt caaatgcaac gtgttccatc attagattat ccggaacctt
    17281 attgtagtgt ttcatcacgt tgatggctgc catccaggca ttgtgcaatg aaaccggggc
    17341 tgaagtcagt gatgttgtta acaacaacaa cagagcaaaa gtcaatactt tggtcattca
    17401 ataaaaattg gaatgtaaag ataacgaaaa ttattcaatc acaacttttt gccagatgtt
    17461 gccgttttcc gaaatcaagc ccacagaata caaacctgag gaaagattat cgatgggaat
    17521 catcatggta ctgccgtcgg ttttttgatt aaagacacag cgtccactga aatcatagat
    17581 gactaccgat gcaccatcgg ctaggttgcc gggaatgatg ttgagcacat tgctggcagg
    17641 atttggaaag acaagcagtg aatacccctc aggacgttcc gaaatatcag tggttgtagt
    17701 atagaatact ttaaatccat cgccggtaat tgatgtattt gattgaaaca aaacctgaat
    17761 tttcgatgtt gccgcgctta caatagttgg gttgtcacct ttgtgaaatt ctccaagaac
    17821 ggttgatgtt attgcgtccg ttactttaac cagatcaaat tcgtcggcca tgtcaaaatc
    17881 aatgaaatga actgaaatgg acgttgctcc tgctggctga atattccagc ggcagaaact
    17941 cgaattgccg taggtatttg ttccgctgcc atcgtcgaat gtgtctgttg gattagtcat
    18001 ggaggttata ccagagcaac cgagtggaat atgtccaaca taattgagca ggaatccttt
    18061 gctgtgattg gtagagttgg aaacaaactg tataaacacc tgactgctgt ttacgttgaa
    18121 ataggccggt accgtgctac ccgaaaaagc gcctgcaact gttgcagttg catcggctcc
    18181 tgcatatatt ttcacggaat cgtcggtggc cagatccagt tcttcaaatg tgattttgaa
    18241 ataatcgcat tgagggcccg ggctgatgag ccaggagcaa ttgcaattgg cttcataatt
    18301 ttcagtgggt ccgctgccat cttcaatcag tccgaaagtg gatgtcagtg ttttagttcc
    18361 tgtgcagcca tacggatacg aagcagaagg aggatatatg ctctcgacac attggtggct
    18421 atcgttgaaa tcgcttccgg ccgggtttaa tgcgttcaga taaaaataat cattggcata
    18481 tccgtcccag ccccaatcaa aatgaaaaaa atctgtacct tcataaccat ccatcacaaa
    18541 agcatgacca ccaccgtcac ctgaacctga ataaatgaca ggtctgagca aatcaaggtt
    18601 attacgcatc atggttgccc aggtagttga attgtaagtc attcggcttc tgtagttcgc
    18661 actttcgtag cggaaataag tggtcaatgc atcgggtaca tcccaggtgt aggctcctga
    18721 tccgtcatag ccatagccca tatcaacggc aacgccgcaa tggtacgaca gttcagccac
    18781 ttcgggcatg ggaaatcccg gatcacctga catttcagaa taattgtagg ttgaagcacc
    18841 aaaattgaca ctttgtgttc catagccata ggcattataa gaatgagacc catagccggt
    18901 ttgcgggaat tgataataat aaattacctg agccatagcc gttgctacgc agccaaccag
    18961 tgcttcgcca ttggcatctt cggggcactg atcattgaaa actccatcct ggccccaggt
    19021 tgaacgtaaa aaaggcagta ccgctttgct tgaatgaaat ggattgttgg ttgtatttcc
    19081 tgaaagtaaa aatccccatt cttcgtcaat ttctgaagtc gattttgttg catgctggcg
    19141 atagtactga atctgctcaa tggcattctg tatccaccag gcaaaatttg atttccaagc
    19201 agtctgaggc acagaattgc gggtgctgta accaatgact ggagtggctg atttgtcgcc
    19261 cgaaatgatg gcaaatccac cgtttggatt attgtagatg aaaatttccg gatttacgac
    19321 agatgtgcca ggaacggaga tcaaaataag ctctgcagct gtggccggca tgattgcatt
    19381 tttggtgctt agaatactga atgcagcttg ccgtgcggtt ttttcagtaa caacctgtgc
    19441 agaaagctga atcaccgaac aaaaggtcag aaaaaaaaga aatgttgtgc tgattttttt
    19501 catgtaaagt attttagcgg ggggttactt agttttattg acaaaaataa ttaagaattg
    19561 ttgcagccga gggtaaaaaa taattacttt cgcaaaaaaa taacatccag agccataaac
    19621 aatgaaactg atttttaatg ccgccgctgt tgctgttatt gaaaatgcgc catcaccaaa
    19681 tccagttgcc ggtaatctga tgaagcagac aaatgttatt gagaatgcat atttattaat
    19741 cgataacaac agaattcatt catttgggaa aatgtctgat ttgaaatctg tgcggcagca
    19801 gttctcaata agtgaagaaa ttgatgcgtc cgggggtttt gtattgccgg gtttttgcga
    19861 tagccacaca catatagtat ttgccggttc gcgcgaaatg gaatttgtcg ataaaataaa
    19921 tggtttgtcc tatgaagata tagccaaacg cggcggaggt attctcaata gtgcggaaaa
    19981 actgcgtcat accagcgaag acgagttatt tgaagcagcc cgtaaacgtg ttatcgaagt
    20041 tatttcgtat ggtacaactg ctcttgaaat aaaaagcgga tatggactga ctccggaaag
    20101 cgaaatcaag attctgcgcg ttatcaaacg tctgaaagag cattttccga ttccggttaa
    20161 aagcacattt ctcggtgctc atgctatacc aaaacattat cgtgacaacc gtcaggacta
    20221 tatccgtgaa atcattgacg ttatgttgcc tcagatcgct gatgaaaatc tggccgatta
    20281 tattgatgtt ttttgtgatg aagggttttt taccatcgaa gaaacgtctg ccattcttaa
    20341 atctgctgct gcttatggtt tgaaaccaaa aatccatgcg aacgaaatgg ctaattccgg
    20401 cggtgttcag attggaatta agtatcatgc agtttctgtc gatcatctcg aacgcattga
    20461 aaatgccgaa attgatgctt tgctggccag caatacaatg ccggttgctc ttccgggtgc
    20521 ttcgtttttt ttgcggattc cctatgctcc ggtgcgtcag atgattgatt ctgggcttcc
    20581 ggtggtgctg gcatctgatt ttaatccggg ctcaagtccg agtggcaata tgcagttcat
    20641 catgtcgctg gcctgcatct atggccgtat gctgcccgaa gaagcactcc atgctattac
    20701 aatcaacggg gcttttgcca tggaaattca gcaggattac ggaagtatct gcccgggaaa
    20761 aatggctaat cttattatta ccaaagaaat tccgtcgctg gcatttatgc cctattacta
    20821 tggtttcaac aaaatcgacc gggttatcat caatggtgaa ttattcgacc cgtccaaata
    20881 ctaaacaata aaatttccga aatgaaacag atcattgaat gtgttccgaa tttcagcgaa
    20941 ggccgcgatc tgacggtcat aaaacaaatc accgatgtta ttgaatcatg cgaaggcgtc
    21001 cgtctgctcg atgttgaccc gggtgcagcc accaaccgaa cggtggtcac ttttgttggc
    21061 acccccgacg aagtgattga ggctgctttt caggcagtaa aaaaagcggc attgcttatc
    21121 gatatgtcga aacattccgg tgctcatccg cgctttggtg ctaccgatgt atgtccgctg
    21181 gttccggtgg ccaatattac catggaagaa gtgacagaat atgcccgcaa acttggaaaa
    21241 cgcattggcg aagaattaaa tattccggtg tattgctacg aagatgctgc tttcaccaaa
    21301 gagcgtcgta atctggcttt ttgccgttca ggtgaatacg aaggcctgaa agaaaaaatt
    21361 gtgaaacctg agtggaagcc cgatttcggt ccggctgaat tcaattgtcg atgtggtgca
    21421 accgctgttg gtgcgcgcga ttttcttgtt gccatcaatt acaatctgaa tactaccagc
    21481 acccgtcgtg ccaatgccat tgcattcgat gtgcgcgaaa aaggtcggcc gaagcgtgaa
    21541 ggaaatcctg ttacaggtaa agtgataaaa gacgaaaaag gtaatgatgt gattatccct
    21601 ggtacactca aagctaccaa agccattggc tggtttatcg atgaatatgg gattgcgcag
    21661 gtgagcatga acattaccaa catttcgaca acaccagtgc atgtggcttt tgatgaggtt
    21721 tgcagaaaag cggcagatcg tggcgttcgt gtaacaggta gcgaaattgt tggtctcgtt
    21781 ccgaaaaaag tgctgattga tgcgggtaag cattatctcg ccaaacagca gcgctcgtta
    21841 ggcgtttctg aaagcgaact cgttaaaatc gccgtgaaaa gcatgggtct cgatgacctc
    21901 aagccattca atccgcagga aaaaatcatc gagtatatgc tcgaagccga atcgaaggaa
    21961 aagaaactgg tggatatgac ctgtgttggt tttgccgacg aaaccgccag cgagtcaccc
    22021 gcacctggcg gaggctcgat ttcggcttac atggccgttc ttggtgcatc gctcggaacc
    22081 atggtggcca acctgagttc gcacaaaccc ggatgggatg agcgctggga agaattcagc
    22141 cgttatgctg aaaccggaca gaaactcaaa gacgagctga tgcatctggt tgatgaggat
    22201 acacgtgctt tcaataaaat catgaatgca cttggattac ccaaaggcac tcccgaagaa
    22261 aaagcttcac gtaaagccgc catgcaggcc gccacgcaat atgccaccga agttcctttc
    22321 aaaaccatgc agaaagctgc cgaagtgtta ccgctttgta aagccatggc agaaaccggc
    22381 aatccgaatt cggtatcgga tgcaggtgtt ggcgcattgg ctgccactgc cggagtacgc
    22441 ggtgcattcc tgaatgtgaa aatcaatgcc ggcggaatcg aggacaaggc ctgggctgca
    22501 gacatcattg ccaaagctca gaaagtgcat gatgacgcat tgacgctcga agctgagatc
    22561 atgactcagg tggagaaggt tattgcaaaa gcatgatgca tgcggtataa agaataggtc
    22621 tacggattac actgattgca cggaaatatt tttgcctcag cggtttcctc gcgatgacac
    22681 tgttgtcaac aattattact gtgtaataat cattgactgg agaatgcgta caacctatct
    22741 gtggaaatgt cttcagtcgc aggctgaaag acatttcatc cgttcaaatc ttaagacctg
    22801 ctgagggtat ttgaatcagt ggaatcagtg gacaagagaa acatgggcgg cgttgaatta
    22861 tttagcaaaa cgcacaagtt tacaacattt tggcgtccgc agacgatgat ttttgcaagg
    22921 gagcggtccc ggaattccgc cagcgggcgg acaggctgtt gggtgctgaa gatgccaaat
    22981 gcccgacgtc tgccttgcca gtcagataaa attccaggtc agcgatgctt tgatgttaac
    23041 ccaatcaagc tgacgctgaa atatcgggga caacccaaca ttctgtcgtt cgcctgacaa
    23101 tgatttgcga acaggagcgg acacggaatc gttgggtgat ccatctcttc ccgacgattc
    23161 cacttcagca atgatttgtt gttaaccact tcttgctgac ggtaaaatgt cgggcgaagc
    23221 acaattcaca cccgatatga aaatgttatt ttatcagcga ttgcgggttg ctgcattaaa
    23281 aaaccgtagc tttgcatctt tttaaaaaaa tccgaaatga ttgcagcttc aaatatttcc
    23341 attcgctatg gaaaacgtgt tttatttgac gaggtaagtg taaagtttac tccgggcaat
    23401 tgctatggca ttattggcgc caatggcgct ggcaaatcca ctttcctgaa aatactttca
    23461 ggcgagattg atgcaccaac gggacaaatt tccattacgc cgggtgagcg aatgagtgtt
    23521 cttaaacagg atcatcacga gtttgacaac ttcactgtgc tcgaaacggt gattatgggt
    23581 aataaacagt tgtggaaaat catgcagcaa aaagacatcc tgtatgccaa gcccgatttc
    23641 agcgatgccg atggtgtaaa ggccgcagag cttgaaacca aatttgcgga gatggacggc
    23701 tggaatgccg aaagtgatgc agccagcttg ctgagcgctt tgggcgtgaa ggaagaagat
    23761 catgacaagc gcatgaagca tatgccggcg aaagaaaagg tgcgtgtgct gcttgccaat
    23821 gctttgtttg gaaatcctga tattttatta cttgatgagc ctaccaacga tctggatgtg
    23881 gcgaccatca gctggctcga agaattcctg attaatttca gaaacaccgt gattgttgtt
    23941 tctcacgaca gacatttcct tgatgccgtt tgtacacatg tagctgatat cgactttgga
    24001 aaaatcactt tgtacacagg gaattattct ttctggtacg aatcgagtca gctggcattg
    24061 cggcagcggg ccgaccagaa taaaaagacc gaagaccgtc gtaaggaatt gcaggacttt
    24121 gttaaccgct tcagcgccaa tgcttcgaaa tcgaggcagg ccaccagccg gaaaaagctt
    24181 cttgaaaagc tggtaatcga tgagataaag cccagtacgc gccggtatcc gggaattatt
    24241 tttaaagccg atcgcgaatg tggcgaccag attttgcatg cagataattt gtcaaagcaa
    24301 tcaagcgaag gattgtcgct gttttcgaat gtttcgttta cgctgaaccg cgacgacaaa
    24361 gtggctgttt tatcgcgcaa tcaccacgct gtttccgctt ttttcaacat tctgatggag
    24421 cttgaaacag cggacagagg cgcgtttcag tggggtagca ccatttcaaa atcatattta
    24481 ccagccaaca acgaccctta ttttaaaaat ccgatgacgc tgatggactg gctgcagcag
    24541 tattcgaccg tcgaagacga tgactcatat atccgcggat ttctcggtaa aatgctgttc
    24601 tcgggagaag aatatctgaa gaaaacctcc gttttgtcag gaggtgaaaa agtgcgctgc
    24661 atggtttcgc gcatgatgct caccaatgca aactgcctta tacttgatga gcccaccaac
    24721 catctcgata tggagaccat cacggcattt aacaatgctt tgaaggactg gaagcacgtt
    24781 gctttattca gctctcgcga tcatgagttt acacagaccg ttgccaaccg aatcattgag
    24841 ctcactccca gaggtattat tgacaagctt atgacgtatg atgaatacct tgaggataaa
    24901 agcgtgaaag cgctccagga gaagatgtat tcgtagcaga agattgttca tgtgaatcgg
    24961 taacaggaaa gatttatgaa ccaggcaagt aatgtttcga aaacctttga cagtgaattt
    25021 gattttaaga aaatcatatt tgtttcattt ataagcagta aaatgaaaaa catcagattt
    25081 gttgtattcc ttacggtaat cacgtctgtc cagcagctca attttgctca gcaaaatact
    25141 tttgatgttc agatgagatt agttaaaggc agtgatgccc atataggcag ctattcctat
    25201 ccattggagc tgaaaaatga gacaggtttt aaaatgccgg ttgatagtgc tcgatgtaag
    25261 accggatatt ttgtaataaa ccctttgctt gcagtttttg aaaaccgcaa cagcagcgat
    25321 tccgccagaa aagtttttga gaatatttta tcgtatattc aaatggacac gctgcaattt
    25381 gttactaatg cattaataac taaccgaatt tatttctgct cctggataga cggagacatc
    25441 aaaaaagttg tcgtcgacac tgattgcgac agctcatttc ggaatgaaga aatcctcact
    25501 tataatctca gcaatggatt gtatgacttt ggagacagta acctggtatt ttccgattgc
    25561 gatctgacat taagatacga ttccctgaat atacacaagc agaaaacgat tcccatccgg
    25621 gtttgtctga atgagaatat tgatgggaaa ttaatgtttg actatgactc gctggaggtc
    25681 tatatctcaa tcaatgcatg tcaggaaggc cgtttcatct ttgaaggaga tactgtggat
    25741 attgtaatga acccaaattg tttcgatcag ttctatcctc agaatagtat ttatggtgtt
    25801 tattcctata gatatcttgg ctatgactat ttaaattata ttactaatga tgagataaga
    25861 ttgttcaaca agttgttcgt cattgaaaaa tatgactatt tcacccgggt gcttaaggtc
    25921 agattcgcag gttctgacag tgtcggcgca taccaaggaa atatttttcc tgtgactccg
    25981 ggtcttgaaa agttcgatta ttattctctg gtattcttta ccggttcatg gtgtggcccc
    26041 tgtaaacatc tgctccccgg actaaaggaa cttcacaaaa acaatccaga atttgaaata
    26101 ttcaatgtta ataatgaaag ggattcaatt gattatttta aatacctcag tcaatacggg
    26161 attgattggc cggttattta tgaccaggat ctggcgaatg tggattcata ttactggcag
    26221 tcatataata tcaattcaat accaacctta tttctcatta acccaaacag gaaaattctt
    26281 gatgtgcaaa cagggttaca taactgtctg cagttattga agaatctgaa agaaaatggc
    26341 atcgagtatt atgaaactac cggaccgtga atgaaaaaca gtgactcata ttggggtatg
    26401 ctgaaaaaca gacacattca gattataaca gtaatacttc tcagctattg atctgcaata
    26461 agtgacagta ttgcgagttt gtctcttgtt gaatatcaaa tgtatttccc caatcttaca
    26521 ttgtcaatcc aactcaatcg taaacgatcc gggatttcct gcggtgccat tgaatgattc
    26581 ctgaaaggtt cctgcggagg cgctggtcca ggtcatcacg tatttgtccg atccaccgac
    26641 atcgaatgta agctctgacg aattgatgcc ttttttcgaa taagtgaaag agaagtcatc
    26701 cacgccggtg agggcagcca tgccactgct gctggtcgat gcaaaatacc attcgttggt
    26761 cggaccgccg gtttcaacca gtgtcagcgt cagctttccc cacgatgaag ttgctattgc
    26821 ataatccgtt gttgatggat cgtcagtgtc atcttcacat gcggtgaata caaatgaaaa
    26881 agtcagggtc aaggcgatta agagaagaaa ttttttcata ggctggcttt ttttgcaaat
    26941 ataaaaagtt ttcggaatcg ctttaaagcg gatgtgttat tctaaatctt ctttggcatt
    27001 ttgtacaccg aatcaagatg attggatgtg ctatgcaaaa tttcaagata ccggttaatt
    27061 tttccaacat attcaatccg ataatcttca tgcatttttt tagttacaag gttgattaat
    27121 ctgtttaaaa gcatggccac ttttgaatca agcgttgtaa aacaagtccc caacagtttg
    27181 tctggattgg agaatggaac ttcgataaca aacagcacta actcggcttc cagcacttta
    27241 tttttcgata ctttggtgaa ttcgttaatt ttccggatgc attctgccag catattggca
    27301 agttgcaatt gaaaagaaaa tcctttatgg ctttttgtaa ataccaggtc caattccttc
    27361 ttcgtttttt cgaacagttc agtctcgcca aagtctttat tgatgtattg tgatgaaaca
    27421 aaatcatagg cgaatttatc tttcgtgatc agttttagta caatttcctg cagttcagca
    27481 gtcgataatt gcctgatatg cgatataaat gttttgtcag gtttgggcat actttcattt
    27541 ttttaaccgg tgtctgtttt gcaaaaatag gagatttgga ttttattggg caatagattt
    27601 ccgtattttt attgagaaca acataaaaat tcagttgttt aattttcgaa tcagcaagtt
    27661 agaagatcat cgcatcatcg gctaagtatt aatcacctct tatttctcat ttcgtgtttt
    27721 ttcacgtctt tcacacttgt catggcttgc ccggttttac gcagttttac gtgattccca
    27781 catacgacga tttatcacgt tttacgtgaa gtcgtcacat atgcgaagcg tcatatcatt
    27841 taatatctcc tatttctcat ttcgtatttc gtctctcgta tttccagcta atcccaccca
    27901 tcagcataaa cccgggctgc gacacattcg catgatcaat gatttcggtg ttgaacaggt
    27961 ttgttgcatt caccgtgaaa cgcagctgct tccactgata aaacacgccg gcattggcca
    28021 gccagactgg ctgatagggg atttcggcca gatagactcc ttccgaaaac ggcgtgtagc
    28081 cgccggcgcg atcctgccag cccaattgcc aggtgagtcc tgcattttca aaaatgcggt
    28141 gtgtagcttt gatatcgagt ttgtgtttca tgtagtcgag cacatactgc gacacaaatc
    28201 cttcgcttga cttgttgctg acaagaaatg tgtaattcac actgacgcgt tgcagaaaaa
    28261 atttctttcc gaaaaattcc ggcaacatcg taaaccctgc ttcaatgccg cttacattca
    28321 ctttcgtcag gttcttgcat tcccatacag tggcggtatc atcgcgaacc caatcgatca
    28381 tattgatacc gtagcgctga aatccagcca cttcggccaa aatatttttt cggtcccatt
    28441 taaaaccgcc ttcaatactg attgcttctt ccggcttcag gagcggattt ccaatgtttg
    28501 tcggcgatga ataatacaaa ttggtgaagt tcggaagccg caaactgcgg ttggctgttg
    28561 caaaaatccg caagggtttt gaaatctgaa aaccaagatc aattccagga taaaggctca
    28621 gatcatgttc caattgatta cttacatata ccagtgcacc acctgaaatg ctgaattttt
    28681 ttgttgcata gcgatgttcg cccataatgc tgaagccgtt tcgctgtgcc gattttgtgt
    28741 agtatgcgct atctgttccg gcaacggcaa tgggagaatt catcgctgtc ccgagtacat
    28801 tgctgaatat gtgttccatg cggaaatcgg cacccagcga tgttttgtgc tttttccatg
    28861 aaaaagtctt gttcaccgag gctcctgcaa catccgataa atgatagttg tggtttttgt
    28921 accaggcagg cggattgttg cggaacagtt cgaagcggtc gtagtgcctg cgccagtaaa
    28981 cattggcctt ccatttttgt gcatttacat cagtcagccc tgccgatgca aaaagggtgg
    29041 aagttgcttc gtactgattg ggatatttcg gcgaataaaa gctgttggcg ccgaaccctt
    29101 tgttgatata tccggcctgt acatcggtct ttgttttgcc aatcgttgca ttcatacggt
    29161 aatatgcacc tgcttttttg aaatcggtgt tttcggtgta gccatcagta gtgctgccgt
    29221 ttaatgacac catgtgatac attttttttg ttctgaaagg caggttgact gacagttcgc
    29281 ccgttccata caatccgccg cgcagcgaaa ccgaaggcaa tgctttttgt ggtgatgatg
    29341 taataatatt gatggcgcct gcaaatgcat tcggcccgaa tatgcgcgcc ccggaaccct
    29401 gcagaacttc aattctttct attgcggtga tatcgacggg aatatccatg ttgtgatgtc
    29461 ccgtttgcgg atcgctgatg ttgattccat tgaggagtac cagtacctga tcaaacgttc
    29521 cgcccctcag cgaaatatcg gattgagcac cggtaatacc gcgctgacgt atgtcgatac
    29581 cccgcacata ttcaagcagg gcgcccagat cgctgaccgg agccaattgt atttcttttg
    29641 cacggataac atgtacaact ctgagtaatt cagactgtac ggttggcgct acctgagcac
    29701 tgataataac cgagtcaaga tttattttct gaatggtatc attttgtgaa cggctggtat
    29761 caacctggct gaacccgttg aaatttgacg aagcaatgaa gaatagacaa aacaaaaagg
    29821 aagctgttct gaaatgcgca gaccgtgaaa gttttgtaaa gcgaaccatg tttggggctt
    29881 tgaaattttg cgcaaaggta ggaatagaga agaattttgt tgcagtttca aattgttaaa
    29941 atctacatgc ttgcaattca actggtaacg attgtcattt atagcaacac attgttataa
    30001 tggctttagt tgcctccgtt catccgggtg ttaatacctc ttgaatttac tggtggtttt
    30061 gtagctgagc actctgttat gatcacatgt ctgaaggagt agataatctt gttctgcgga
    30121 tctatgggag ctgtcagtat ctgttttgtt tctttgggtt tcagttcaat atagtcagaa
    30181 cattcttttt tactgcttac aataattcca ctttgatttt tcccttcagt aatatacgtg
    30241 aagcggaatg tcatgctttc agcaccattg ttttgtgctt taacaatacg tccctgttca
    30301 acagataaca ctatatcgca gcgaaattga tttctctgtg tagtaccata tggcacaaag
    30361 ataaatatgt aaacaatcgc gataaataat attttcatgt cgattaaaaa atttatttca
    30421 aagataaaac atgttgtatt taatatcaaa tacagaatgt taatttattt ctgggttttt
    30481 gttatttagg aatgccggat ttttaaaaat aagacagctt tgtcacaaac ccattgattt
    30541 tcgctgccga cgcttcgacg gagtttaccc cgctgttcgt gcttcgacgg agtttatcct
    30601 gctgttgact cttcgacgaa gtttatcctg cgggacgcag ggctcagcat gacagcaggc
    30661 agggctcagc atgacagcgg cggggctcag cgtgacagcc tcttttttgt cattttttat
    30721 ttaggttgca aaacgcgttt tggtggtgct tgtcgcgtaa tgtcagcatg actgcacgcg
    30781 ggtctcagga tgacagcgag ggaatggtgt tttctaaagg attgcagaga gatttttttc
    30841 ttcattttag ttgtaaaata ctccaagcag agtgtttaaa attgactaaa atccggatta
    30901 ttcttgtgat gatacggcag tattccaaca aacaattctc tgaataatat tttaagttcc
    30961 aatgcagtcc catatcctaa aaaatcagat atttgcatca ctttaataac caaaatatat
    31021 aatcatgtcc ggaaaaactt ttgccgagaa gattttcaat gctcctgcgg ggagcatcgt
    31081 ttttgcgaaa cctgatattg tgctgtctca tgacaacacc gccagcattg caggcacttt
    31141 taaaaaaatg aatggtgaga aggttgctta tcccgaccag attctcatag tgcttgacca
    31201 taatgcaccg cccactacat cggggctggc aaacgattat cagactatcc gcaatattgt
    31261 gaaagaacag ggaattaaaa aattccacga tgttgggaaa ggaatctgtc atcagatgat
    31321 ggcattgcac gcacgtccca aaatgcttgt agttggttct gacagtcata catgtacggc
    31381 cggggcattc aattcttttg ctgccggaat cgaccgtacc gaaacagctg gcctctggaa
    31441 agccggcgaa acatggttcc gtgtgcccga ttcagtgaaa ataacattga caggaaaatt
    31501 taatcaaggc gtgtatgcca aagatctcgc tctctggatt attggtatga ttggcagtgc
    31561 aggagccgat tacatgtcga tagaatatca tggcgaaggg gtaaaaacac tttctgttgc
    31621 cgatcgcatg gtccttgcca atctggcttc ggaaatgggc gccaaaaacg ctgttttccc
    31681 ggctgatgaa gtgcttgaaa aatatttcgg acaaaaattc gaaggcctct gggccgatgt
    31741 tgatgctaaa tatatccgcg agatcaatat tgatctcacc accatttttc cggtggtttc
    31801 tgctccgcac aatgttgaca atgtaaaggc tgtgagcgaa gttcagggca ccgaactgca
    31861 acaggctgtt atcggaacct gcaccaacgg tcgtattgaa gacctgcgtg aagccgcagc
    31921 tattctcaaa ggaaacagag tccctgattt cttccaattg ctgattatcc ctgcgtcgca
    31981 ggaaatttat tcgcaggcat tgcacgaagg attgattgaa atttttatgg ctgccggcgc
    32041 caatattctt acgccttcat gcggtccgtg tcttggcacc ggacaaggaa ttccggccga
    32101 cggattcaat gttatttcaa cggccaatcg caatttcctg gggcgtatgg ggaataaaaa
    32161 cgccagcatt tatctggcct ctcctgcaac tgttgctgcg tcggcactgg ccggaaaaat
    32221 cacggatccg cgcggtatcc atggcaacga taaatttccg tacagcatcg agcaaagtcc
    32281 gacggtttcg attcctgaag gcgaaaatcg cttgcttgga aacacctgga attacaccga
    32341 tgtgaacaac ctgaatacgg accagatgtt tgccggtaac ctgacttaca atatcaagag
    32401 cagcgaaggc gataagatta tgccgtatct gttcaaaggt tttgacgagg catttgccga
    32461 aaaagtcggg gcagggcagc tcatcatggc cggagccaat ttcggttgcg gaagctcgcg
    32521 cgaacacccg gctgtaggtc ttgcttttgc tgatgtgaaa gcagtgatct gcaaatcggt
    32581 caaccgcata ttctaccgtt catccatcaa tcagggactg ccgatcatcg tgctgcccga
    32641 tgcagtggat gcatacaagg ccggagatgc tgttgaactc gatttcgctg caggcaaact
    32701 cactgtgggc gacaaagtgt tcactttcaa tccgcttcct gaaaagctga tgaacatttt
    32761 cgccaacaaa ggtctggtga attattacaa gaaaaattag gtcttaataa aaccttgaag
    32821 acttgtcaaa taaaacggtg ctgtcatgct gagcggagtc gaagcataga tagcaccgtc
    32881 ttctcttcga agcaatttaa cgtagttgtt tccttttaat gatttgcacc aatgcactac
    32941 tacgacatcc atacacatca cgaatcgcag tctccattgg tgacaggcat attgaatgtg
    33001 attccgggac agtccgttcc ggaatcattt ttttcagcag gaatacatcc ctggtatgcg
    33061 aaagacgctt ccacagcgaa tttggaacaa ctaagcacaa atccttattg tgttgccatt
    33121 ggtgaatgtg ggttggattc agtaaaatcg gccatttcat ttgatgaaca aaaaatactt
    33181 ttccgtgctc acctggagct ggcccggcaa tttccgaaac cagttgtttt gcatgttgtg
    33241 cagagcacca atgaattgtt tcagctcgtg aaagaatttc ctgagctggt gtatatctgg
    33301 catggattca ctggaaacga agaaatagtt aaacaatttc ttcgtttcaa catgtatttt
    33361 tcttttggtc agcgaggatt gaaaaaacct gaaagctggc tttcaatgcc ggaaggcaga
    33421 ttgctttgtg aaacagatga ttcggacatt ccgattgaac aaatgtatgc agaacttgct
    33481 gccatccgaa atgagtcggt ggagcagctt tcgaaaataa ttgaaaacaa cgtattcaga
    33541 atatttggac atgagttgga atgaaaggac tgaactgtta ttgggcagcg atgctgttga
    33601 aaaactcgta caatcccatg tgttgattgc tggactgggc ggggtaggcg gcatggtggc
    33661 cgaaatgctg gtgcgtgctg gcattggcga aatcaccatc atcgatgctg acaccattca
    33721 tgaatcaaac cgaaaccgtc agctgattgc gcttagcagc aacgacggac accgcaaagc
    33781 ggaagaatgg cacaagcgtt tactcgacat caatccggag ctgaaagtgc atgtgattgc
    33841 tgaattcatc cgcgacaaag ccattcccga attgctggaa aacaaatttg attttgtggt
    33901 tgacgccatt gatacactgg caccgaaagt gtttctgatt tatcacacca tgcagaaggg
    33961 ttatccgctt gtgtcgagcc ttggaagcgg aagtaagatg gctccggaac aaatcaggat
    34021 atgcgatatt gcagaatcat ataattgcaa actcggattt tatgtccgca aacgactgca
    34081 taaactcggc atccgcagcg gctttgatgt ggtattctca cctgagccgc aaaaagaaaa
    34141 ctccatcatt ccggatgaga gtgacgaagc catcaaatcg accatcggca ccatttcata
    34201 catgcccaat gcgttcggaa cttttctcgc aagtgttgtg atacggcgct tgctgggaat
    34261 tgaaatcaat aaatagaagc atttcaataa cgaaactcac ttatattcac ggttaccatt
    34321 tttccgggct cagtcagact ctccagatgc ttcttgtaaa caacggtgtt gtcgtctata
    34381 aaataaaagt ctcttcctgg aatggattca cactctttaa tcacgaaata attgacgggt
    34441 gcttttagtt ttctgggagg aagagatcca tcggcggtaa tcagaaataa cagtccatcg
    34501 caggaattgt tttcggcatc ggaaggtgta atactcagaa caagagcgag agaagagtcg
    34561 ggcgaccaag ctatgccgca tagagtatgg ggaccgtttc gcacaatcag aatttcgcgt
    34621 tttgaacgat ggcttatatc atattcaaac actgtgtcag catcgctgta atacaggaaa
    34681 ttgctgtcgc agagcgaaac aaagcggggc gaaaacaagc atttctgtac ttcgtatggc
    34741 agcgatattt tcatgcccga agtatcggtg actatgtaat cggtataaaa tgacgttgcg
    34801 actttttcaa gaaaaaactg cgcccggcct tgttgcatgc aaagcaataa aaacacaaat
    34861 aacaatgctg tgcgcagctt gctgtttaac gttgaatgat tatttgctgt gtgtggttcg
    34921 atgttgcaga aattatacgt agcaaatata caccttctgc cagaattgaa gtgttaatat
    34981 tacctgcaaa ttcgcaatta attgcttttt gcagaacagt ttttccactc atatcactga
    35041 tcgtcagcag ggcttgattg gcagtagcag gaagaacaat gtacagatta tcctgtgccg
    35101 gatttggata taccttaatg gggtcttcgt tttcaatcaa tccggtgcaa tcaataaaag
    35161 taatagatgt tgtatctgat gaagcgcaac cattggttgt aacaatgacg gaatattcaa
    35221 atacgccata accatttgta agagctgtat ccaccaacaa agtgctggta gttgagccgt
    35281 ttgaccagag ataagtatcg gcgcctgatc ctgcatcaag cagaatactg ccgtcaccgc
    35341 agaaggtggt gtcggctgcc agtgttactg ttggatatcc gaacgattca atgatctgca
    35401 tgatggcatt gttgcttgaa tctgcatcgt gcgaattgta gagttgtact tcgatgtagt
    35461 aagtgtatgg attgacggtc atatcaaccg gagcactgaa tgtgtgtgta ataatctgct
    35521 gcggcagaaa cggtgctgac agtacaatgg tgtccatcac tacattgagc cagccctcat
    35581 ttaatgcaac gaatatggta tcacctgcag gaattgtatt tgcaaagttg gtattctgca
    35641 gacgaatggt aatactgtca atgccgtgtt cacatgcatc agataatgtg tcagccgata
    35701 acatggcgat atcgtaaacg ggattttcat acactgaaaa atcatcaaaa gcgaagccat
    35761 cattgcctcc attcattgct gaaaaaacaa aacggaatct gacccatggc tgacctgtga
    35821 gcatcgaaag atcgtggcgc gccagtttcc atgtgctctg actttcaccg gtccatcctg
    35881 ttgaatcggc cagtgtagcc aaagccgata tgtgttcgca gttgtaccag ttgagcgtgt
    35941 cgcctgcatt tccaatgatg ttccatgtta ttccatctgt tgtgtattga aaagttgttc
    36001 cgtcaaataa tgtatctgaa tttgcaaagt atttgaattc gacatgaggg agcgacatgt
    36061 tggtcatatc gaagcatgga ctttcaatga tgcttgtcgg agttccgctg aagggcccct
    36121 gattgcaggt agcatatgca tttgaaccac tgaatgctgt gttgatagtg gttccggccg
    36181 gtacaccctt tgtccagagt gtgtcctgtg ttgaccaaac ctgaatggat tcagcatcat
    36241 caaaataggg ataagcagaa atcagtggct ggctgatgat ggtttttgca gctgagtcat
    36301 tggctgtatt aagatcgagg ggcagcattg attttacaat aattgtgtgt ggcccggctg
    36361 ccgaaaaatc aaaagtctga ttggttgtaa catttgttgt tgtacctgat gtaatatttg
    36421 ttgcaatggt atcattcacc caggtggcac cgccatcggc tgaaatccgc acagggagcg
    36481 atgttacagt aacagttcca tgattgatgg cgtcgaatga aactacattt atccctaatc
    36541 cgcaattgct ttcgggtgct gttatgattg aaatttccac atcgtttccg cggtccgaaa
    36601 tacggatatt gtcaaatgca aatccttcat cgttattaaa actgccggca ccgaaaagaa
    36661 cacgcagacg aattccggaa tagtttgtaa gattaaacat actgtgctgc atggtaatcc
    36721 aaccattgct tccattcaca tcggcgccgg tccagccggg agcattgttg gcaatgccgt
    36781 ctatatcgta atcgttgtac cagttggcat cgccatagtt gcccagtgtt tgccaactgt
    36841 ttccaccgtc ggtcgaatac tgcacttgtg cgccgtccca gcccccttcg gcttcgtacc
    36901 atgcatccat ttcgagccat ggatcgctca tggagcccaa atcaaaacaa ggactttcaa
    36961 cataactcag ttcattcagg ttgtgagtgc cggttagatt ggtcacccag cagtttaatc
    37021 ctgccgatgc tgtatcaata attgtgtttt cgggctcacc caattgccag gaattgttgg
    37081 tgccccctga aacccagctt ccagtgccag attcaaagct ttcgacgtaa ggaaaaatat
    37141 taattgttgt gagtgaactg atgagaacag aatcgatgtt attgtttgga ttttcatcgg
    37201 aaagtggatt tatctgaacc tgaaaattgt atagtccggg tgtagttaaa tctgcagaat
    37261 ttgtaaagct gtaagtgatt gtatctcctg aggaaatggc gggcaaattt tcaggtgagc
    37321 tccaggttga tccgccattg attgaaaatt tcgcaacaac gttggattca ggcattgaac
    37381 cgttgttgaa aaaggtggct gttactgttt cagtgcttcc aagcccgcat cccgaaacag
    37441 gggcgtcgat tgacaatata accatatcgt gatcaagcaa ttcaaattca taggcgccca
    37501 tatccggatt tgtcagattc cgtgcggttc ccatgatatc ttcagtcact gttgtcatcg
    37561 gcagtcccag attattgaga gctgcattgg tgaattccgg cgatgttgct cctgcaaata
    37621 atggatcaat ttcaatggag tgcgtgtcga aacctgcagc tgtagcggct gtacgccatg
    37681 ctgcaatatc agtgcagttg gtcgaaccgt atttgccgag ataactgccg ctgacattat
    37741 agttgttgta gtcgcattga gatacataat tattggtgat ggaagttgat gaaatctcaa
    37801 tgccatagcc gcctccgttg ttctcgaaaa tattattcag gatgcggata ttgccgtatc
    37861 ctgtggatga attgatgtaa attgctcgtc cggctgtagt gcttccgctt tgaatgcgta
    37921 tggtgttgaa ggcgacatca acataattgc agttgtaagg ataaataccg cgtaatgtgg
    37981 cagtattgtt ttgttgataa ataaaattgt tggcaatcag aaccgggttt gcacttgtac
    38041 cattgaaatc gcgcatatag agcatgtctc cggcagctcc atcggccata atggtgttgc
    38101 gggttactac aacatttcct tttccatatc gcagataaaa tccactgaaa ttcgaagtaa
    38161 cagcattggt gactactaca ttgctgtcaa tgatggtgtt gctttgattg tacatgaaaa
    38221 ttccggaaac ataagggttg atgatgttgt tttttctgat aacaagcgag tcctccgtat
    38281 tggatgatga tgcgccatac agatacattc cgtatgcacc gttttcaatt ctgttgtttt
    38341 caaaaatact gatggaatca tttgtcatgc ttcctgggtt ggagtaaata actgcgagag
    38401 catttgagtt tactgttgct gcaggcgctg tcagatgcag attgcgataa tgattgtacg
    38461 cacaggtgct gtcgatggta agtacacgcg aaaaagaagt atttcctgaa cttgcaagag
    38521 tgaggtcttc tatggtgaga aaagaggtcg catttattga tagtgtatag ttatccgatg
    38581 catcggttgg catatgtgtt attttaacgg cagcactgtc atgcgatgcg gatcggagga
    38641 caatgttgtt gattggcgaa acgccataaa cacggtctat tctggcttgt tcagtgtatt
    38701 gaccgtcggc tatttcaatt atgaccggac cacagacacc gccatcagaa agcgtatcac
    38761 atgcggcggc aatggattga aaattatttc ctgttgctgg ctgtgaagca tcaacggtgt
    38821 acaatccgtt caatgaaata tgaatccagg tgcgtagtgt atcattgctt ttgttggtgt
    38881 cggccgaatc cagataaact gtcagcagat acgcaccggg ccttgcaaat ataaatgtgt
    38941 caagctccag tgtgtctttt tcgtcaggcg atatggctgt tgcagggcca ttcagcaaac
    39001 ctgaggtacc attggttact gtccagtgaa tattgagggc tccggcagaa attcctgttc
    39061 cgcctgaatc agccagaatc acatgattat acaccggtga tccgaaacag tagtaattct
    39121 gcgatggttc ggcaaatgtg ctcaatgaat aatcctgctg tgcaaatgag ctgaaacata
    39181 caagactgaa aatcattgtc acgaaaacag catactgctt tttcatatta attgttttta
    39241 agttcttcaa ctatttttgt gagaatttca cctgctttnn nnnnnnnnnn nnnnnnnnnn
    39301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39361 nnnnnnnnnn nnnnnnnnca tgaaggacag actggtggaa ttttgtccag cattttttca
    39421 tcggccggat attttgcatg gcatttttcg cacagaagtt gcgcggtcgt tccatgaaac
    39481 tcaatcacat cggtgtttcc ggccatatga tgaaaatggt cgatattctg ggtaatgacc
    39541 agaagtttct tttttatttt ttcaatttcg gccagtgcat aatgtgccgc attgggcttt
    39601 gtgttctgca gaaaactgta gaacaaatct tttattactt tccatgactc agccggatgg
    39661 ctgtagaagt aattcaattc aagcacttcg ggattgtatt ttgtccacac tccgccttca
    39721 ccgcgaaaag tcggtattcc gctttcggcc gatatgcccg cacctgtgaa tacggccggc
    39781 ttctgcgact cacggattaa ctgagcagct ttttgaattc cccctatcat gattcaaatg
    39841 tatgattttt atttgaatca tttttatttt attttttctt caggaagctc tgtttttgtc
    39901 aaattctgtc attaagcccg tggccttttc aaaatcttct tcggcaacat acatctttat
    39961 tccgccgatg gcatttgata acagcgggtc aacgttgatg gtcatctcat cacacatgtt
    40021 tatacgaatg ttattcgatt ccagaaatgc ctgcaacatg tagtattcat gcggccagac
    40081 aaagcttcgc aaaaacaccc agttaatttc ttcgttcatg ttttttttgt aaaaataaaa
    40141 aaatccggcg aaccggattc cttttcaaat taaaactgaa tgttgcgtca caacttctca
    40201 ttaaatcgct tgctcactgc tatccagtca atcactttaa agaagtcgtc tatatacgca
    40261 gctcttttat tctggtgttt cagataatag gcatgctccc atacatcgag cgccagcagc
    40321 ggatttacag tgcctgccgg aacattcatc aatgggttgt cctgattggg ggtagtgaca
    40381 attttcaatg ctcctttttc ctgaatcagc cacacccatc cactgccaaa tattgataaa
    40441 cctgcatttt tgaattcctc aataaatttt tccatgcttc caaattgtgc attgatgcgg
    40501 ttcttcaatg ctccctcagg aattgcactt ccaccgggtg tcatcgattc ccagaaaaat
    40561 tcatggttcc agaaaccgcc gccgttgttg cggaccgctg ccggaaattc attcatgcgt
    40621 gccattatct caggcagtga aagcgattcg agatggtggt ttcctttgat ggctgccatg
    40681 aatttatcgt agtatccccg atgatgtttt gtataatgaa tctccatagt ttttgcatct
    40741 atgtatggtt ccagcgcatc gtaggcatag ggcaggggct tgaatacatg agtttgtgcg
    40801 gtggttccga agaacagcag caatgcgaaa atgacaacaa atattttcat gctttagtta
    40861 tttaatgatg gacaaatatc ttttttcaac ttcggaccag ttcactatat cccagaaagc
    40921 tttgatgtaa tcgggacggc ggttttggta atgcagataa tatgcgtgtt cccacacatc
    40981 aagtccaaga ataggataac ctcgtttttc ggctacattc atcagcggat tgtcctgatt
    41041 cggagttgaa cttataaaca gattattttc ctcatcaatt gataaccatg cccaaccgct
    41101 cccaaagcgt gatgctgcag ctttgccaaa ttcgtccctg aattcttcaa aaccaccaaa
    41161 actttgtgtt atgtgtttca ttaaatcgcc ttctggttta gaaccattgt ttatttttaa
    41221 aattttccag aagagtgagt gattgtagtg tccgcctccg ttgttgcgta cagccggcgc
    41281 cagtttatcg gctttgtcaa ataaatgaag caagggtttg cttatcaatg catcgtcgcc
    41341 cagcgctttg ttgaggttat ctacataggc ctgatggtgt tttgtgtgat gtatttccat
    41401 cgtgcgtgca tcgatatgtg gctccagtac tgaatattcg taatccagtg tgggaagatt
    41461 gtatggaaaa tcaagacgaa attctggtaa tatttcaagc tttttcatga cgtttatttt
    41521 ttgttattta gaacaattca aaattacaac agaatctgtc ctgcccgaca taaattccgg
    41581 agacaaaaat tgatattaat catgccatgg tcaacgcaat aaatattaca agaacgcaag
    41641 ctcatcgacc ttcaagagtc cgcaatgcaa tgaaggacct ggaagagtcg gatgctgaga
    41701 cctcatacca actattccat gttctccgat tctctgcgaa ctatggattg ttggattgcc
    41761 cgattattat aagatatgct gtaattgaga tcggcttttg ccatacaaaa tcaactctgt
    41821 gcggcttaaa tggattatat ttgtagtcca tgattgtttc agtgacacaa gcgtgggagt
    41881 aattgtctgt gaaatgtttt tcgtctgatt agtttttcca tactatgaga ctatttttta
    41941 aatccgcatt acttgttgca atccttgttt caacttcgtt tactgcgcag gcacaacacg
    42001 tcgatacaac tgatttcaca cctggatatc ctattgtgtt acgtggaata gtaatcgaca
    42061 aagtgacaca tgattcagtg ccgctggcca ttgtcgaatt gctgattaat tctaagggaa
    42121 ttcatgccac aaggagtgat tttgatggcg gattcgttct cagtctttgt tcgggacgtc
    42181 tgaccaaagg attgcttcgt attcaggtga ccgtcaaagg ctaccagcag gatatttttg
    42241 agtttaaagt cgagggagat acttcggtac gcatagaatt gacgcctgat ctggaaggga
    42301 agattactga gcaggaggtg tggatgaaat tcgatgatgt gattgaggcg aaatatgata
    42361 tctggggcgg atacgagaaa ttcaggcatt gtgacggacg aatcaaaaca tatgatgaga
    42421 ttattgaatc aggtgaaaaa tggctaggtt gggagccgta tgattttgat atttttgatc
    42481 cggaataata tgattatcta tccgtggaaa tggcttcagt cgcaggctga agctatttca
    42541 tccgttcaaa atccaaagat ttgccgaggg ttgtgaaaca gtgaaatccg tgtaatcagt
    42601 ggacaaaaaa atgtacattt gctataagta tcatactcat gaaacgtttt ttatcagaag
    42661 ccatacagct gggccacgcg aaatacgaac aatccgtttc gcggcagcag gtaatttata
    42721 aaaagagtga agacatccgc accgattttt cgcgcgacta caaccgcatt ctgcattgca
    42781 atgctttcag gcgtctgaaa cacaaaacgc aggtgtttta tgcaaccatg aacgaccata
    42841 tttgtacgcg catcgagcat gtgcatcatg tcgaagctgt gtcgcgcact atttgcgaag
    42901 agttgggttt gaatgcagaa ctcggcacgg ccattgcgct ggggcacgat ctgggtcatg
    42961 ctccgtttgg ccatgaagga gaaaaagtaa tttcaaaatt atcgaaggag tttttgcaac
    43021 attctttttg gcacgaacgc aacagtttgc gcgtggttga ttttattgag agtttgcccg
    43081 atttgcaggg tgtagaacag ccgctgaatc tgacatatgc tgtgcgcgac ggtattatat
    43141 ctcattgtgg cgaagttgat cagaatgggc tgaaacccag aaccgaagcc attgatctgc
    43201 attctattcg ttatcctaat gaatatccgc cttacacctg ggaaggttgt gttgtgaaaa
    43261 ttgccgataa aattgcttat ctcggccgag acatcgaaga tgcccagcga ttgaatatac
    43321 taacgaacga gcaggtgaga gagcttaacg atctgattcg acagaagtat ccgtcagtta
    43381 ccgagagcgt ttttacgaca tcgctgatgc ataattttat tattgatctg tgcgaaaaca
    43441 gctcgctaaa cgatggactg atgctagatt cagatacatt tggtttgatg agaagcatta
    43501 aagatttcaa ttaccagtac atttataatc atccacgtat ccggcgctac aacaggtatg
    43561 tggaactcat tatatccacc ctttttcagt ttatgctcga tttgtatgat ggagaaaata
    43621 cagccgataa tattgaaaag gaatgtgaac aatatccgga attacagaat tcttttggcg
    43681 aatggattcg caaatacact aagcaaaacg gcaaccacag cgcgtttgct attttcgatc
    43741 tgacatcaaa ggaatcttat attgattctg ttctcacata tttgtcgctt acaaccgatc
    43801 agtatgctat gcgtttgttt ggcgaagtga ttcatttttg agtctggtca taaatagaag
    43861 tcaattctta gcggtgagct gcgagcagtg agtagtaatc ggctttcagc caggtcaaga
    43921 acgaggtcaa ggacaagcag cttccagtca tcagttgtca gctttcagct ctgagacggg
    43981 gcttagggta tagcatgcgg catgcggcta gcggcatgcg gagcacaacg ctttcgttct
    44041 gaattctcac ggagccctgc ctgctgtcac actgagccct gcgtcccgca ggataaactt
    44101 cgtcgaagtg tcagcagctg gataaacagt gtcgaagcac gaacaacggg ataaactcca
    44161 tcgaagattc gctctgtaat tggcatgcgg tgcacaatgc acgaaatgct caattcggca
    44221 ctcacttcaa aaatcaaaga tcaaaaatcc ttgcctgcct ggcagtcaga caggttctta
    44281 aatcaatcct tccttgttcc ctgttcagag ttcaatgatt atttctgcag aatccacaat
    44341 ccttcattgc aagcttcttc cttcattttt tcaagtgttt tcaaagcatc gtctttgctc
    44401 gaaaagctct gatatgatac ccggtaggat ccggttgaat tctgaccaac aatttcagca
    44461 gatggataac cgccggcgcg aagttcatcg cggaattttt ctgcgttttt caagaacata
    44521 aaactaccgc caatgatgta ccagcgcggg ttgtagtcaa tcaatacatc attatatctg
    44581 tttgcactga tgttattggt gagattcaga ttggcttcgg tcagataatg ccccaacgtc
    44641 ataccatcac tgactgagcc ggcaatgcgg aattctgtgc ggcggttcaa ctgacgtcca
    44701 gcatcggttt cattggtggc aatgggctga tcaaatccat atcccatgta ggtgagtctc
    44761 tgtgcatcaa taccagctga tacaagatac tgaacaacag ccatggcgcg gttttctgaa
    44821 aggtttttat tgtaagaccg cgagccataa ttgtcggtat gccccgaaat ttcaattttc
    44881 aaatcgggta atgcaatcag gagacttaca agccggtcga gttctgtttt cgactcaggt
    44941 cgcaaggttg atttatcaaa atcaaagtaa acatttcgta atacaaaacg gtcgcccatg
    45001 cgctgtccaa cattgatgcg ttcaagaatg gtgtcaggtt caatggctac ggtgtgtccg
    45061 aaataatagg gtttataaaa aaccagttca tcatttcctg aatacggaat atcaagtaaa
    45121 tcctgaattt gcataccacc agcagtagta attaccactt tgtatttatg cccgcttttt
    45181 agtgtggcac tgaaattccc gctttccttg tccgattcgg tttgcaaata gatttcattg
    45241 gttactgcat caaatacggt aactcttgat tgaagcggaa cattcattac ggtatctttt
    45301 accaatccgt tcagaatggc aatttgttct gtttgtccca ataccataac cctgtaaata
    45361 tccaggtcac cataacactc tccaaattcc gatgcataat atgcctgttt cccatcgagg
    45421 gtcggtataa aaaaaacatc atcattgact gtgttgagcg ggtaaccgat gtttcttgga
    45481 aaactccatt cgccattttc aagaatggaa taaaaaatat cgtaaccgcc catgcattca
    45541 tgcccttcgg agctgaagta caaggtcttt ccatccgata aaatcatggg ggaatcttcg
    45601 ttatactccg aatttacaga tgggccgagg ttgacagctt tgccccattt attgtgtcct
    45661 attcgtttcg acatccagat gtcttttccg ccaacacctc ctggccgcga agaaacaaag
    45721 tagagtgttt ctccatcagg actcagacag gcgtggtttt cgtagtattt tgtattgatt
    45781 tctccaccca gtttttcagc agcagaccat tgtccatctt ctgttttgct catgtaaata
    45841 ttcccgtctc ctttgtcgtc gcggtagata aacagtgtag atccatccca cgacatgctg
    45901 atgcttgctt cgtgtccgct tgtatttaca ttgccgggca acatttgcat gttgctgaat
    45961 gttttgttgt tttcgtcgca tttggcaata taaatgtctt caaaatattt ccctgtctga
    46021 tccttttttc cgcccgttga acctttacgg cgtgaagtga atataaaaaa atgctgatcg
    46081 ggtgacatta ccggcgcata atccggatat tcagaattga tgattttacc aaggttttca
    46141 atccttacct gaatcgggtt ggcaatcaga ttgcgtgcat tatatgtcaa tgcgatctgt
    46201 cggtttacat cttttatcaa ctcatcgtct tcgggcgtga ggtagtatct gaatttatcg
    46261 aaattttcca atgccaggtc aaatttctgc tcgtaaaaat atgctttgcc gagaaagtaa
    46321 taagtgaata ccggcgctgt cttctcattg aaatttccgt agtagtccgt tgaaatattt
    46381 tgcgatgcca gttccagata tttcgacgct ttgtcctttt cgtcaaatga ttttaaataa
    46441 cacatcccaa gataaaactg aatgttggga ttgttggggg ccatgctgtc gagtttttga
    46501 aaacaaggaa gggcgtcaat atagttttcg gcataaatat aaccatcggc catttcatac
    46561 aagcgcttaa aactgctgtt gaattgagct gaacttgtat tgatgaaacc aataaaaatg
    46621 aatacgaaaa ttagatattt catgatggat agagttttgt ctgtaacaaa attacgcaaa
    46681 aatcatggcc gctcaaagct atttaataca tgttgtcaac aaagaagtgt taagtaaatg
    46741 gataaagtaa actgattccg ccatgccttt agctgattat attggtaaaa aaagttattt
    46801 tttgtatctt tgtgaagata actgaaaatg tcatgaaact acgttttgta tataccccga
    46861 agccgcggca gtttacctat aaaccgcaat ttttcaaacc ggaagaagat ggaaaaaaaa
    46921 tattttcaac cgaaggtccc ggtaaaatga cacacgaggc ttatcgccgc tacagaagag
    46981 atacgtcaaa aagcaaacag aaaaggaatc agagtgtgtt gatttatgta gcaattattc
    47041 tgctactttt gtactttatt ttcttctgat ttttctatgt ccgatatcat aaagcttttg
    47101 cccgaatcag ttgccaatca gattgcagct ggtgaagttg ttcaacgtcc tgcttcggtt
    47161 gtgaaggagt tgctcgaaaa tgcagttgat tcaggggctt cgtcaataga cctttttgtg
    47221 aaggactccg gaaaaacatt gattcaggtt gttgataatg gccgtggaat gagtgttgcc
    47281 gatgcccgta tggcctttga acggcatgca acttcaaaga tatgccgcgc cgaagatctt
    47341 tttgctattc attcgtttgg atttcggggc gaagcgcttg cttctattgc ctctgttgcc
    47401 agagtcgaaa tgaaaacccg cgacgaagtt tccgaggccg gtatcagtat tgtaattgaa
    47461 ggttcaaaat ttatttccca ggaaatctgt gcagcaccca aaggcaccaa tattcaggta
    47521 aaagacctgt ttttcaatat ccctgcgcga cggcagtttc tcaaaagcaa taatgcagaa
    47581 atgcatcata tctgggatga aattcacagg gttgccattc ccaatcatca tatccggttc
    47641 agaatatttg aaggtagccg cattctttca acccatgagc catccaatct gcagcaacgg
    47701 attgtttcac tttttggaaa acaatatcag cagcggctta tgtctgtttc gcaggacacg
    47761 cagtatgtcc gtgtcagtgg tgttgttatt cgtcctgaat tcagtaaaaa aacacgtggt
    47821 gaacaattcc tttttgtgaa caaccggttt atccgcagca attatatcaa tgctgccatc
    47881 aaaaatgctt atcaggggct gattccggcc gatgcatttc cgggctattt tatctttatt
    47941 gaggttgatc ctgctgaaat tgacatcaat attcatccga ctaagactga aatcaaattc
    48001 agggatgaac gaatgatagc caccatgatt acaagcacta tccgccggtc gcttggcact
    48061 gggaatgcca tgccttccat cgattttgaa gtagaacagg gaatcgattt ttcgcttaat
    48121 cctgatcgtg aagtgaaaat tccccaaata aaaatcaatc ccgagttcaa tccattccgc
    48181 aattctgttc aacccatcga ttatacaagt gccgaacgct cccggctgca ttgggaaaac
    48241 gacaccattc cttcgccgga tccattacca gaggacacat tgccagatca gtttgtgcct
    48301 caaaacgaaa ctttcaatac agaatcttat attcaggcag gacgccgctt tattatcagc
    48361 aatgtgaaat cgggattatt gcttgtcgat cagcatcagg ccagtctgcg tatcgttttc
    48421 gaccaacttg tcaattccgg tcatcatgaa tctaaaaatg cgcgcctgct tatgtttccc
    48481 gaaaatattt cctttggagc tgctgatagt gaattgatgg ctgctatcag tgatgaactc
    48541 aataactgcg gttttgtcat ttcgcagttg ggccgcgaca tgttcagtgt cagtgcggtt
    48601 ccggacgatg tggaattgaa tggaatttct gtaaagcaac tattggaggg tgttctggaa
    48661 gattataaat cggcaggggc tttgcgcacc gatcggcgtg aagctgtcat tttgtcaatg
    48721 gcaagaaagt tgtcagtgcg tcatggccaa tttctcaaca gcgaaatgat gaagttgatg
    48781 atttcaaagc tttttgcttc atcaaatcct gaaatatcac cggaagggaa aaatattttc
    48841 accattgttt ccgccgatga tcttgcgaaa tggcttcact aaaggatttg aataaatgaa
    48901 tgattattac cgccccggcg gattttcgat gatgccaccg gcagtgaaaa atctgctgat
    48961 tataaatgtt ttgtttttcg ttgctacctg gattttgcgt gataaaggta ttgacctgta
    49021 tcagatattg ggtcttagct gggttcaatc cgaaaacttc agaccgtggc agtttatttc
    49081 ttatatgttc atgcatgccg attttacgca tcttttcttt aatatgtttg cgttgtggat
    49141 gtttggatat ctgatcgaaa atttctgggg cgcaaaacgt tttctcactt tttatttcgt
    49201 aacgggtatt ggtgcagctt tggttcagct tggcatcaac tggtttgaat tcagccacct
    49261 gcacaacgtt cttgaaattt acgcatccaa tcccaatatt actgatttta aggaaattgt
    49321 tcagcagcat tttgcctccc gcgaaaatgt cgatgcagcc aataacctac tgcgcaattg
    49381 gagtatgagg cccgatgata ctctgtttat tggtcaaagc atggaacaac tacgtctgat
    49441 gatggaaggt agaatggcca ttcctatggt tggcgcatca ggagctgttt ttggaatttt
    49501 acttgccttt ggaatgatgt tcccgaatca gcgtatatat atttattttt tgtttccaat
    49561 caaggccaag tattttgtaa tcatttatgg agctattgaa ttcttttttg gtatttcagg
    49621 cgctcagagt ggtgtggctc attttgcaca tctcggtggt atgttgttcg gttttctgct
    49681 gatcatgtac tggcgcaata aacataaatt cagaaggtag ccatgtttta tcatcatccg
    49741 cagcagggag gttttctcaa tcgctttttc agtttcatgt tttccaaaca ggccgtttct
    49801 cgtctgattc tgttcaatac cattgttttt attcttgttt tgctgaatgg cttgaatgat
    49861 tttttgttca aactgaaaaa tgtatatccc gatgctttgt ccgcattggc ttattatctg
    49921 gctttacctg ccgatataaa tcagcttgca gcccgcccct ggactgcttt tacagctatg
    49981 ttcctgcatt cttcgttctg gcattttctg cttaatatgc tgatgctgta ttttagcggg
    50041 gttatctttc tttctgtttt gaataaccgg aaactctgga tttcatattt cattggtggt
    50101 ttatctggag ccgtagcatt tgttgccgct tacaattttt ttcctgtctt tgaaaatatc
    50161 attcccatta gttatgctat cggagccagt gctgcggtga tggcggtttt gtttactgtg
    50221 tctgcctatg cgcccaatat gtcgttacga ctttttcttt ttggtcagat tcaattgaaa
    50281 tatctcgcgc tgatatttgt gctgatcgat gtattgagca tcactgcttc gaacccgggc
    50341 gggcatattg ctcatctggg gggtgctttg tttggcgtta tttatggatt atggcttaaa
    50401 tcgagctctg taggtggttt ttcgatgaat gctcgattga aaaaaatgtt taagcgcaaa
    50461 cctaaaatga aagcgcgggt gaataatcac aacagacctg aatccgatga tgaataccgc
    50521 aatcgcaaag ctgccgataa caaaagagtg gatgctattc ttgataaaat atcaaaaggc
    50581 ggttatgatt cattaaccaa ggaagaaaag gattttttgt ttcgacacgg gagaaaataa
    50641 tttctgcttt attctggcga tttaatatat tgtttattct gaacaggatt cggatttttc
    50701 tgctattttt gtaaaaaaac aaattcatca tgggaagagt acttatcatc ggcgcaggtg
    50761 gcgtcggaac cgttgtagca cataaatgtg cgcagttgcc tgaagtgttc agcgaaatta
    50821 tgctggccag ccgcaccaaa tcaaaatgcg atgccattgc tgcaaaaatt ggcggaaacc
    50881 gcatccagac tgctgctgtc gatgccgata atgttgaaga acttacaaag ttattgaaag
    50941 aatataaacc tcaactggtc attaatgtag cacttccgta tcaggatttg catattatgg
    51001 atgcttgtct gaatgcgggc gtcaactatc tcgatactgc caattacgaa cccatcgacg
    51061 aagctaaatt tgaatacagt tggcagtggg cctatcagga tcgctttaaa gaagccggtc
    51121 tcacagccat tctcggttgt ggattcgacc ccggtgtaac cagcatctac acggcatttg
    51181 caaaaaagca ttatttcgat gaaatgcatt atctcgacat cgttgactgc aatgccggtg
    51241 atcacggaaa atattttgca acgaatttca atcccgaaat caacatccgc gaaataacac
    51301 aggatggcct ttattgggaa aatgggaaat ggattgagac caaaccgctc gaaattcata
    51361 aacccattga atatccgaat attggaccgc gcgaatcata cgtgatgcat cacgaagagc
    51421 ttgaatcgtt ggtgaaaaac tatccgcatt tcaaacgtgc acgtttctgg atgaccttcg
    51481 gacagcgcta tatcactgcg cttgaggtgt tgcaggaagt tggtctgacc tccatcaagc
    51541 ccatcaaata ccagggagta gatattgttc cgctgcagtt cctgaaagca ttgctgcccg
    51601 atcctggaag tcttggtgag aactacaaag gtgaaaccag cattggatgc cagattcgcg
    51661 gaatcaaaga tggaaaagaa cgcacttatt acgtatggaa taactgcagt catgaacagg
    51721 catacaaaga aaccggcgca cagggcgtaa gctacacaac cggcgtaccg gctatgattg
    51781 gtgctaaaat gttccttgaa ggaaaatggt cgggtgccgg tgttttcaat gtcgaagaat
    51841 ttgatccgga tccgtttatg gctgacttga acaaatatgg tctgccttgg caggaagaga
    51901 ttgataaacc gcttccggtc gagaaatagc agtttataaa gttcttaaag ttcataaagt
    51961 ggaaaggcaa cgtattaagt tgataaccag gaagactcca tttgtgaata ttgttctgaa
    52021 atgttttaac tcatgaagtc gttttaatta tttttaaatg aacattgaaa aatttgaaga
    52081 cattattgca tggcagcgtg caagagaatt gactaaactg gtcaactgca attttggaag
    52141 ttcacgagat tcgaattttc gcgaccaaat atgtagtgca tcgctttcta ttatgaacaa
    52201 tattgcggaa ggatttgaac gaaagtcaaa caatgaacta aggcaatttc tgttttattc
    52261 aaaaggttcc tgcggcgaag tgcgttcaat gttgtatgta gcattcgact tactatatat
    52321 atcgaaagaa agatttctgt tactaatgga attatgcctc gaaacatcca gattgctttc
    52381 tggatttatc aaatccctga atacaacaaa taaagtgtaa aaattgcttt atgtgccttt
    52441 ataaactttt cactttataa actttacaaa cttgaaagta aaatgcataa ttcaattcca
    52501 agcccatgct tcgtaatcga agaaaaaaaa ctgcttgcaa acctgcagtt gatgcagaaa
    52561 gtaagagaag aggccggcgt tgatattctg gttgcgctta aaggaatgtc gttctggcat
    52621 tactttccac tcatgaagca atatcttagt ggcgcttgtg ccagctcggt gaatgaagcc
    52681 cgtcttattt ttgaagaaat gggtgtaaag gcgcatacct atgctccggc atacgaggaa
    52741 aatgattttg aacaactgct gcaactgagt cagaccatca ctttcaactc gctttatcag
    52801 tggaataagt ataaagataa aatgctgcaa catcccgaaa tacatgcagg attgcgcatc
    52861 aatccgggct attcggaaat cgaaaccgat atttacaatc cggcggttcc gggctcacgt
    52921 cttggaattc cgcttgagca gctgcctgca acattgcccg aaggtatttc gggtttgcac
    52981 tttcatgtga tgtgtgagca ggattcgtat gtgttggagc gtgttttaaa agtggtggaa
    53041 gaaaaattcg gacatctgtt gaaacaggcg cagtggttca atatgggtgg tggacatcat
    53101 atcacccgtg ccgattacaa accggaacat ttgattgcat tgttgaaaga ttttaaatcg
    53161 cgctatccga atctgaaaat tattcttgaa cctggcgaag ctgttggctg gcgcacggga
    53221 ttcctgcttg ctacggtgct cgatatagtt gaaagcggcg gaatcaaaac cgccatcctc
    53281 gatgtttcat tcagcgcgca catgcccgat acgctcgaaa tgccctacaa acctattatt
    53341 gtaggggcag gggagccaaa tgaattttct tttaattacc gaatgggtgg ctccacctgt
    53401 cttgccggtg atttttatgg tgactacagt tttccaaacg agctgaaacc cggcgacagg
    53461 attgagtttg aagacatgat tcattacacg atggtcaaga ctacactatt caacggcgtt
    53521 cgtcatccgc atattggaaa gattgactgt gacgggaaat tcaccttgct gcgctcagta
    53581 ggctacgaag aatataagaa aagactttcg tagtttgcgt tacagtcgtg acacgttaca
    53641 aacgttacag tcgttacgcg tggaatttat cacgatgaaa gcgggaatcg ttacgaaatt
    53701 gctttcaata gacagccgtc agcagtaggt gccgaccgaa gcgtcggtgc tctgagaggg
    53761 agctttgaag aaggcttgcg gcatgaagca tgcggcatac ggttcttcgc gacacaaatc
    53821 ttaacgttga gaaaaatcat ggacaaaggc gattacttat caatcacatt aaatttcaac
    53881 cgaaaaggga aatgcaaagt ttcattgctt attgtatcag ggatgcagta taaacctacg
    53941 attgtgtttg gaccaatttt gcctgtttta agtgatagtt ttgcttccca gctgttttga
    54001 aaaatagtgt cacattttcc aatcattttt agattaatag tatcaagtgc atttcctaca
    54061 accaaatatg gtattcccat gtgattccca tttttaatca tatcaaaggc tgaaatatta
    54121 acttgagcgt gaagtgaatc tccaactact acagttgggt tttcagaaaa tgcatttact
    54181 gcaatttgtt taaaattaaa tgcattatta ttttctgaaa tttgtcggct atattttatt
    54241 gagtcagcct caattttcaa ctccaacaat tctatttgat tcgttttttt atgcagaata
    54301 cacaatagaa atacaattat tgaaatcagc aatcctgcga ttaaaagata aaacacgata
    54361 gatttcttca tgactataat ttgaaaacaa agataagaaa cgtcaaaaaa ccgcaaccat
    54421 actggttcaa atcagattgt cttcagtgta attgctaagc cataaaatcg tattaatcca
    54481 aattcgcaaa tcagatcaaa aatctcaaat caacaatcgg atatcttaaa tcatatggcc
    54541 gtatgcttcc ctctggccca cgaaaaccag gttccgagca gcagtaaaaa aaagaaaatt
    54601 ccaaacacgt attgcatcac atcgaccagc cggagcgcgt ttgcagcatt cactctgtga
    54661 gcaccgatga gccagctgat gaaaagtgca gccattccca tactgatcat ttgtcccgaa
    54721 atgcgcatgg tgcttagtgt tgccgatgct gttccgaatt gcggcggttc aactgaattc
    54781 atgacggaat gtgtattggg tgttgagaaa aaaccaaagc caagcccaag aatggcaagc
    54841 gctgcataga agaattcatg cggagtttca gcagttgcaa acacaagcat tccaacgccg
    54901 atggctgaca gtaacatgcc caatgatgca agaatacgcg aatcgtactt gtcgctcatg
    54961 cgcccgctga tggttgcgga aatggccatc acaaccggtt gaatcataag aatggctccg
    55021 gttgcggcag gactgaggcc gcgcgtgtac tgaaaatata gactcagcag aaaaccaacg
    55081 gcaaaagtag ctgcataatt gataagtgca gccatgttgc tgaatgcata gcgtctgctt
    55141 gatacgaaaa atttcacatc gaaaaccgga tgaagtatgc gatattcata aatgataaat
    55201 gcaataatgc cggcagcccc aacagcagtt aaaagttgtg cttctggttg tatgatgttg
    55261 gcaaaaccat acattaacat gctcatgaaa acaacataaa tgattgatcc gatccaatcg
    55321 aagggtgtat tgcgacccgt tttccagtct tcgcctgaat aaaacatcag caatccaata
    55381 acaagagctg agttgacaat gctgacataa aacagtgatt gccagttgat aaattcaatc
    55441 agaaatccgc ccagaacggg ggctgcagat aatccgatat agacagcggt tacattataa
    55501 ccaagaaatt ttccacgttt ttcttttgga aaagcagaag taataatggc ggtaccggtt
    55561 acaaaaagca tagatgaacc aattccctga atgaatctga gagccagaaa gaaaaaagtt
    55621 tcctgtgcaa aaatacatgc tacagaagtc aacatgacca gataatttcc tgcaataaac
    55681 atttttcgtc gcccgatgcg gtcagccatt tttcccagcg ggatgcagaa tgctgcagat
    55741 gcaagaagga atgacatatt gacccagctg agttcggcag cattcattcc cagatcgcgg
    55801 cttaattgag gcagcgccac attcatggct gccgaaagaa aaggactcag cacgctgctg
    55861 atgatgattg aaagtagcag aagtcgttga ttacgcatgc gcaaaaatac aaaaaaggcc
    55921 gcctgtgttg cagacgacct ttctctgatg ctttgcgtaa attattttac ttttttggcc
    55981 agttctgtac cggctttgaa gcgtacaaca ttttttgcag cgattttgat ggttttgccg
    56041 gtgcgcgggt ttttaccgtt gcgggcagcc attttcttca ctttgaatgt tccgaaacca
    56101 acgagtacaa cgttgttttt ctttttcaga gagcccttga caacttttat aaaagcttcg
    56161 atggctttct ttgaatcaac tttcgttaat tttgcttctg ctgcaatagc atcaactaat
    56221 tctgctttgt tcattttaat tgggttttaa atgattaaca atgcaaatat agcactatta
    56281 ttcaattagc caatgttaaa tgtttgagaa atgtgatgaa atcacaaatg tataaaactg
    56341 catatgtctt tattacctct atcagctatc tctatgtttt taatatatcg cataatgttg
    56401 ctctgtttat cttatagaga aatccatatt caatcgatat attttttatt cgatggttgc
    56461 ttgaaacgcg cattcagtgc ggattttaat attattctct ttaatgacgg attttaaaac
    56521 tctctgattt gaagatggtt atatggagtt tttttcttga attgattctt gttgtttttg
    56581 atgtcttgcc ttgaatagaa aaaatcgtaa agtaatttat cagtgtttca tgtttggatt
    56641 tccatatcct ttaatcgcgt ttttagctta tatgatgaat ttttcctggt caatccgacc
    56701 atgtggtcag gttgcatcag attgttttgc atatcgcgaa taaaaaaaag accgatcctt
    56761 gaggaacggc cttttcttcg aattagcgaa ctaattactt aacgctggta gctaaagctg
    56821 cacctgcttt gaatttaacg actttcttgg cagcgatttt gattttcttt ccagtgcgtg
    56881 ggttaacgcc ttcacgagcg cttcttttgg caactgagaa tgaaccgaat cctacgagtg
    56941 aaattctgtc accttttttc agagcttttc ctgtaactgt catgaaagca tcgagagctt
    57001 tttttgaatc agctttggtc attttgcttg cagctgcaat tgcgtcaatt aattctgctt
    57061 tgttcatttt tctttgggtt ttggttaata ataaagtttt gaacacgcaa atataaggcg
    57121 taaaaacgat ttgtcaagac ttttttttca aaaaccgcat ttttgttgat aaaatggcta
    57181 tttggttaat aactcctcga taaattcaag ggattttata gacaaacaac acgtttatac
    57241 gattatttta aaatggcgcc gtttaataaa cctgcgtaac ctcttagaaa ttcggcagtt
    57301 gtcatcatac tgcgcccgga tagttggagt ttattcactt gcagcattcc ttgcccacaa
    57361 acaacatgaa tgtatgtttt accatctgaa atgattgttc cagggctctg tgagctggtt
    57421 tcagtcatgc cggaaccggc atctgaaatc ttgagaagtt gctctgtgcc attttcactc
    57481 atcagcatcg tccaggcgcc tggaaatgga gataaacctc gtattttatt aaatatatcc
    57541 tttgtcgcct gatgccagtc aatgcgcgta ttttccttgt ttaatttggg tgccggtttg
    57601 ctgtcttttt gaatattgtt ttgtgctatc gcttttgcag tgccggattc aactgtatcc
    57661 agtgttttga gaataattga tttacccatc tctttcaaac gatcatgcag ggtgccgaaa
    57721 ttatcatttt ctgaaatttc aacggcctct tgcatgatta tatcgccggt atctatggtt
    57781 tcgttgatga aaaatgttgt aagtcctgtt tgtgtttctc cgttgatcag tgcccagtta
    57841 atcggagcag ctcctctgta ttgcggcagt aatgatgcat gcagattgat ggtgcctttt
    57901 gatggaatgc gccatacggc ttccggcaac atacggaatg cgattaccac aataaggtcg
    57961 ggatttattt cctttattat attaaggaat gcagaatctt tcagattaac aggttgcagc
    58021 agcggtaaat catgtgcatc ggcaaatttc tttacttccg aagcagtgag ttttctgccg
    58081 cgacctgcag gtttatctgg cgcagtaaca acagctgcaa tttcgtgccc agcattaaaa
    58141 atagcttcca gcgggccaac cgcgaaatcg ggtgttccca tgtacacaat tttcagcata
    58201 aaaaatttgt tttaacggag aagttttttt tgcgaaacca tcaaaatggc tgttgcagcg
    58261 gcttctacgc ctttgttacc atgttttcca cctgcgcgtg ccaatgcctg ttccatattg
    58321 tttgtagtaa gaacgccaaa tattacagga atggcatgat tgattcccac ctgagcaatt
    58381 ccactggcca cggcctgact gatgaaatca aaatgtctgg tttccccttg tataacacag
    58441 ccgaggcaaa taattgcatc gaagcagttt tttcgggcca tccactcagc ggcaacgcag
    58501 agttcgaaac tgcccggaac atgaatggtt tgtatattgc tttcttcagc tccgcactgt
    58561 agcaaaagtc cgttggctcc gctcagcaag gcatttgtca cttcgctgtt ccagtccgaa
    58621 acaacgatgg caaatctcat tttttttgcc gatggcagag ttgtactgac atcggacaga
    58681 ttttttttct taaccgccat tttattattg agcagagttc ttcattgccg tagctcgggc
    58741 aatatattta ttgatttcgc tgccttcgaa tgattttcga tagtcgtttc tgattttggt
    58801 gtaaagttca agggcattgt cccagtcgcc catggcttca tatacccatg ctgcacgcat
    58861 taaatacatg ggtgaggtga atgagttttc agattgctcc gatgcgtcca gatagtattc
    58921 cagagcttct tcctgattac cgagttccat gtaggcatct ccgatgcagc cggtggccat
    58981 tggcccgatg atatcatcgt cgccatcgaa tttttcgagc tcctcaatag catcttcaaa
    59041 caaaccttgc ttgagataaa tcattccgac ataataatgt gcaagatttc cggaaggtgt
    59101 tccgccgaaa ttatcaataa tctcgagaaa tccggcgtga ttaccgtcgc cgttcagagc
    59161 cagattcagt gaatccattt cataatacat ctgagccatg tatatttcat tttcagcttt
    59221 ggtcactttc ggaccatgaa tgaaattatt gtatccccag attcccaaaa caacaagtgc
    59281 aatacctgca atcacatatg tgatgatatt cttgttcttt tcaatgaact gttctgcctt
    59341 tgaaagggtt gatccgatgt tttcgagtct ttcgtcacct gaaggtgtgg tttgcttggc
    59401 catattctta aattcaatat ttcaatgttc ggggtgcaaa attaatggtt tttttcaaac
    59461 aacagtcatt ttgaaacagt tgcctcttta ttactgttcc tgattgctct tattcagtca
    59521 gatttttttg atcggcctca tttttcttgt ttttcgttgc tgaagaagat gtaattgtaa
    59581 gctcatcatt ttcattgaag taatccataa taatatccgt aggtgcttca aaattatgtt
    59641 tgatgagctc ttcggccagc gggtcttcaa tgtatttttg aatggcacgt ttcaacggac
    59701 gggctccgta attgggatcc catccttttt ctacaataaa gtcacgggct tcatcagtca
    59761 gcttgagcaa gatacctttt tcctgcaaac gtttcaaaac gcctgaaagc tcgatatcga
    59821 tgattttatg aatttcattt ctttccagat tattgaaaat caccacgtca tcaatcctgt
    59881 ttagaaactc aggtgcaaaa gcttttttta atgcattttc aagaacactt tttgcgtagc
    59941 cggctttgga agactctttt gagctggttg caaatccgac tcctgacccg aattctttca
    60001 attgacgcgt cccgatattt gaggtcatga taatgatggt gtttttgaaa tcgactttgc
    60061 gtccaagact atcggtcaac tgtccatcgt cgagaacctg cagcaataaa tgaaatacat
    60121 cgggatgtgc tttttcaatt tcatcgagca ataccacgct gaatggcttg cggcgcactt
    60181 tttcagtaag ctgtcctcct tcttcatagc ccacgtaacc cggcggtgcc ccgattagac
    60241 gtgataccgc gaatttttcc atgtattcgc tcatgtcgat acggattaat gcatcatcgg
    60301 tatcgaaaag atattttgaa agaacttttg ccaggtatgt ttttccaaca ccggtcggtc
    60361 cgagaaaaat aaagctcccg atcggacggt tcggatcttt gagtccgaca cggttacgtc
    60421 ggatgctttt ggcaattttc ttaatggcct cattttgtcc gatgactttg tcacccaatt
    60481 cattttcgat cttgagcaat cggtcgcttt cattctgagc aacacgtgta accggcacac
    60541 cggtcatcat ggcaacaact tcggcaacat cttcatcagt tacaacttcg cggtgtttgt
    60601 cagcttcttc atcccatctc aaacgtgctt tttcaagctg atcgagaaga tttttctcct
    60661 gatctcggtg ttttgcagct tcctcgtatt tctgcatttt tattgctgca gttttattgt
    60721 tccttgcttc atcaatctgt ttttcaagat caagaatttc ctgcggtaca ttgaggtgct
    60781 tcagatgtac tcgcgatccg gcttcatcca atgcatcaat cgatttatcg ggtagtgcgc
    60841 ggtcgctgat ataacgctca gtcagatgaa cacaggcctc aatagcatca tcggtatagc
    60901 gcaccagatg atgatcttcg tatctttcct taatattttg cagaataatt ttcgtttctt
    60961 caatcgaagt tgcatcaaca agaatctttt gaaaacgacg ttccagtgcc ccatcttttt
    61021 caatataatt gcgatattca tcgagggtgg ttgcaccaat gcactgaatt tcaccacgtg
    61081 ccaaagctgg tttgaacatg ttggaagcat cgagtgagcc actggctccg cctgctccga
    61141 caatggtgtg aatttcatca ataaaaagaa taacatcccg gtttttttca agctcattta
    61201 gcaatgcttt catacgctcc tcgaattgtc cgcggtattt tgttccggct accagcgatg
    61261 ctatatcgag catgacaaca cgtttgttgt ggagtgtgcg gggaactttg cgtgctgcaa
    61321 tccgcagggc aagtccttcg gctatggctg atttgccaac acctggttcg ccaatcaata
    61381 caggattgtt tttcttacgt cggctcagaa tctgagcaat acgttccaat tccttgtcac
    61441 gacctacaat cgggtccaga cgaccttctt cggccgcttt ggtcaggtcg cgtccgaaat
    61501 tatccagtac gggcgttttt gatttggatt cagcccccgt ttttttagaa gagcttccaa
    61561 aactgctgcg ctcatcagcg tcgtcatcgt ccgacggcag ttcgtcttta atcctttcag
    61621 aataatgggt gtcgttgcct gtaatattgc gaagctcact gagcatcaga tcgtaggtgg
    61681 caccggttct gccaagcagg gttgaaacat agtttttgtt ttctttcaga atggcgagca
    61741 gcaaatgttc tgtttcaatc agttcgcttt tcagatcttt agcaaccaga taggtcagtt
    61801 tcagaatctg ctcaacctgt cttacaagtg gaatgtcggt ttcgggcata atggcaggac
    61861 cgtcggtttg attggctgcc ttttcaatgg attttcttat ttcaccgagg tttactccgg
    61921 catttcgcag caggtgaata gccatgccat ccccttcgcg cagcatgccc aaaaataagt
    61981 gttctacacc tacataggga ctgttgagac gcaatgcttc ctctttgctg aatcccagca
    62041 catccattac acgttgtgaa aatttagctt ccatatctga gttctgaaat tgatcacaaa
    62101 attacgatta atggttcaaa gataccagta ttctttgagc cggaaaatga tatttatccg
    62161 tttttttaaa cagtggagtg ttattaattg catttgaagt gaacttttta taaatcaaac
    62221 aattgactca cgaatgagct ttttgtcagt cattgtggaa aactattttc atcctttcag
    62281 tatttttttg taatttcgcg tgctattaaa aaagtgtgac gatattgtca aaatagttga
    62341 taaagagaat tttacatgag cgaatttgag aactccggag aaagaattgt acaggttaat
    62401 atcgaagacc agatgaaatc ggcctacatt gactattcaa tgtcggttat tgtttccagg
    62461 gctttgcctg atgtcagaga cggaatgaaa ccggttcacc gccgcgtatt atttggaatg
    62521 catgagttag gcgttttttc gaatcgccct acaaagaaat ctgctcgtat tgtcggggaa
    62581 gtgcttggta agtatcatcc gcacggcgat acatctgttt acgacaccat ggtgcgtatg
    62641 gcgcaggaat ggtctcttcg ttatcccttg gttgaaggac agggaaactt cggttctatc
    62701 gatggtgaca gcgcggctgc catgcgttat accgaagccc gtttgagaaa aattgccgaa
    62761 gaaatgctgg ctgatatcga aaaggaaact gttgatatgc agctcaactt tgacgattcg
    62821 cttgaagaac ctaccgtatt accggccaaa gtaccaaacc ttttgctcaa cggcgccagt
    62881 ggcattgccg ttggtatggc taccaacatg gctccgcaca acctgaaaga ggttgttgat
    62941 ggtattgttg cttatatcga tgataatgaa atcaccatcg aaggtctgat gaaatttatc
    63001 aaagcgcccg attttcctac cggtggcatc atttatggct acgatggagt taaggaagct
    63061 tatgaaaccg gccgcggacg tattgtaatg cgtggtgaag caaatgttga agatggtgac
    63121 aaacacacac aaatcattat caaatcaatc ccttatcagg tcaataaatc tgaactgctc
    63181 aagaaattcg ccgatctcgt caatgaaaaa gttattgatg gagtgaccga tctgcgcgat
    63241 gagtctgatc gtgacggcat gcgcatcgtg cttgatcttc gacgcgatgc agtgcctaat
    63301 gtaattatca atcagcttta tcagcactcg cagttgcaga gcgcttttaa tgtcaataat
    63361 attgcactgg ttcatggacg tccgatgatg cttaatctga aagatctcat taagcatttt
    63421 gttgatcatc gtcacgaagt cgttgtccgg cgtacacaat ttgaattgcg cgaagccgaa
    63481 aaaagagctc atattcttga aggattgctg attgctcttg ataatataga tgaagttgtt
    63541 gaaattctcc gttccagtaa atcagtcgat gaggcccgtg agcggctgat ggagcgcttt
    63601 tctttgtccg atattcaatc cagggctatt gtcgatatgc gtctgcgttc tcttattggt
    63661 ctcgaaagag aaaaactgag agccgaatac gaagagttga tgaagcgtat tgcctggtta
    63721 aagagtgtac ttgaagattt tgctctccgc atgcaaatca tcaaggatga attactcgaa
    63781 atcaaggaga aattcggtga tgaaccacga accgatattg tttatcaggg aaaaaatttc
    63841 agaattgaag ataccattgc caatgacagt gttgttatta cgataagccg aatgggttac
    63901 atcaaacgca ctctcttgtc ggaatatcgc actcagaacc gtggcggaaa aggtcttaag
    63961 gccagtaatg tccgtgatga agatattctc gaaaatctct atatcgcatc aaatcacgat
    64021 tatctgcttg cctttacaaa aaaaggaaaa gttttctggc ttcgcgttta cgaaattccg
    64081 gaaggggcaa gagccagcaa aggacgtccc attcagaatt tgcttgctat cgaaaatgat
    64141 gataagattc aaacggttat taatgtagct gatttgacca ataaggaata tgtcgagaat
    64201 aatttcatca ttctttgcac agagcatggc gtaataaaga aaaccagtct tgaggcctac
    64261 agccgtccac gtcagaatgg catcaatgcc attggtatcc gtgaaggtga cactcttatt
    64321 caggccagtc tgacgaatgg taaaaacgag gttcttattg cactgaaatc gggtcgggcc
    64381 attcgttttc cagaagaaaa agtacgtcct atgggccgta atgccagcgg agtgcgtggt
    64441 gtaacactgg ccggagccga cgataaagtc gttggaatgg tatgtattca ggataaattg
    64501 gaacagattc tggttgtatc tgaaaaagga tttggcaagc gttccgatat tgaggattat
    64561 cgcgttacca atcgtggcgg caaaggggtt aagaccatca atgttaccga aaaaaccggt
    64621 aaacttgtag cagccatgtc ggtaaaagac ggcgatgagc ttatgattat caatgtgagc
    64681 ggcattgcca tccgtatgtc ggtgagcgat ttacgcgtag ttggacgtgc cactcaaggt
    64741 gtcaagctga ttagtctgtc gggcaaagac agcattgctt cagttgccaa gattgcatcc
    64801 gattttgttg aaattgaaga aatagtggaa gaaaatgtgg aagaaaacgg atcggatctt
    64861 gccggaaacg aatcttctga aacaccagac gaaaccaagg agtaaatttt tggcaatgtc
    64921 tttgtaaaac tcatcgaaat cgtacttttg taaactcttt taaaaattca tatcatgaag
    64981 aagttagcat tgtttgttgt tgcacttgtt ttttcaggag tagctttttc ccagaacccc
    65041 gctgttgtta gtgcctttaa ctggctcaaa gacaataaac tcgataaagc tcttgctgcc
    65101 attgaaccgg caacaacaca tccacagacc atgtcgagtg ccaaaacctg gctttacagg
    65161 ggaaacattt acctgaatat tgctctgacc gacaacgaaa gttttaaggc attgtgttcc
    65221 aatccgcttg attctgccta taattcctat cagaaatcca tcatgctgga taaggattac
    65281 attgctccaa cagccaatcc caacagcgct aagctcggac ttttttactt gggcgaccag
    65341 tatttcaatg taggtgccaa ccattacaat aacaaggatt gggctgaagc tcgtaaatat
    65401 ttcgaacgta ccaaacaaat caaaaatacc tttggtgaaa aagactcact ggctactttc
    65461 tatgcaacaa attgcgcgct gcaaatgggc gacaatgaaa ctgccatgaa gtatctgaaa
    65521 gagcttatca acatggatta tcagaaaccc gagatttatt ccatgcttgg caacctgtat
    65581 aaagatgctg gtgatacaac taaaatgctc aacaccattg caatggggcg taaacgtttt
    65641 cctgccgatc tgggccttat catttccgaa acaaactatt atcttggaaa gggtgaattg
    65701 gccaaagctc aggatttgct taaagtagcc gttgaaaaag atccgaacaa cccgatcctg
    65761 cattttacta ttggaagcaa ctatgaccgc ctttcgctcg acacaaatgt ggccgaagca
    65821 gaaaaagaat caatgctggc tgaagctgaa aaagcttatc tgaaagccat tgaacttaaa
    65881 gctgatttct ttgatgctta ttacaatctc ggcgctttat tctttaataa aggtgttgaa
    65941 gtttttgaat tggccaattc tattccaccg gatcagtttg atgcatacga aaaagctaag
    66001 acgaaatata tggaatactg gaacaaatca gttccttatc ttgaaaaagc aagtgaactg
    66061 atgccggacg atcttccaac gcttcaggct ttgaaatctc tttatgcacg tttgaatatg
    66121 cctgaaaaac tgaaaactgt tacagataaa atcaatacgc tgaaacagta ataaatattt
    66181 tacttgtaaa atccggattc tgaaaaggtc cggatttttt ttgcactatg cttaatgcat
    66241 caagcatgtg gcatgcggtt aatcgctcga tatcagcggc ctcgcatgca aacgagtatt
    66301 aagaagcttc attacagtcg ttttgagacg cccaagtgta attcttattt ttttttacta
    66361 cagaaatgtt actcatccgg agtctatcaa acatggacta ctattttctc tttggtatta
    66421 aatctatcag agggccaagc ctgaaactga gagctgatct ctgatccgct gcggcagact
    66481 ggcagccact caaccttaac cacaacctta accttaacct caaccttaac cttaacctta
    66541 accttaacct taaccttaac cttaacctta
//
LOCUS       DAOPKE010000004        63453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_467_length_63453_cov_12.045080, whole genome shotgun sequence.
ACCESSION   DAOPKE010000004 DAOPKE010000000
VERSION     DAOPKE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 63453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_63453_cov_12.045080"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2873
                     /locus_tag="PKY63_00950"
     CDS             <1..2873
                     /locus_tag="PKY63_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99208.1"
                     /translation="IWAVDSVCYGETIDIYATGSETYTWNTGSTNDSIQITVVSDTAI
                     SVVATYLTCIERDTIYIKVKPLPVITFTGDSSVCLNDSMHLGVDGGINYIWENGFTTD
                     SISFLPVASETLTVTVTNSYGCTAQDSIQTTVYPLPVPLITGDNTPCLHDTTSLSASG
                     GVSFEWSNAATTPTIDIAWNSTGTFDYNVIATDLNGCSDTAFFTATVTNLPEFWLGND
                     TIICDLTNITLDPGIAGASYNWSTGAVTQTLDVSSANTYWLDLNDANNCHFRDTIDIA
                     VQALPDISFSGTDTTCFESTITITATAPSGVNYFWSNGMNTSTITFTPAASGLDTISV
                     TVTDALTCSKTDSLEFMVYPLPLPVIAGNPTPCLHDTTTLTASGGESYIWSSGATTTS
                     LDIPFDSMGTYEYFTIATDIHGCSDTAFYTASVLPLPVFDLGADTTICESTSISLHPG
                     ITGAIYDWSTSETTQSINVSLANTYHLEITGMNGCRYSDSITVAVQPYPTITFGGDTT
                     VCYSHPITITASGGVSYVWENSLSGDSIVFTPLSDDTLSVLVTDIYGCSSTDSISFEV
                     LPLPTPNISGDTEICQFDTTTLTASGGITYQWSTGQNTSTASILPSVAGINQIYVIAT
                     GANMCYDTAFHQITVHALPAINLGPDTTICEGTSLLLDAANPGSTYSWNTGASSQTLT
                     ITAAGTYFVSVADANTCENTDSIVISTQPYADASITDISYVCLNGSPFAFVAAQGGGS
                     WSGDGITNASSGEFNPAVAGVGYSIIVYTISGMCGDKDSTTIEVADIPFIDYTLSDET
                     CPGYNDGTLILEISGGTSPYFYDLSGTTITDTTINLAPDGYLLTVTDSRGCMTSDSVI
                     IHAEDTPCGEVELFIPNIFSPNGDLENDVLFVRSNFIETMAWYIYDRYGEKVFESKDI
                     NLGWDGEFEGQKVQNGVYFWHIKATMIDGSVIEREGNVTVVR"
     gene            3004..3630
                     /locus_tag="PKY63_00955"
     CDS             3004..3630
                     /locus_tag="PKY63_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPE99209.1"
                     /translation="MNSELSKTDGRMLPVMEEFPSLQGEGVNTGKAAWFVRLGGCDVG
                     CSFCDVKESWNPDIHPLSPADGIVENVVKSKIGAVVLTGGEPMKWNLDYLTGELHKKS
                     ITIFLETSGSEKPSGTFEWICISPKKGTIVDSYWFEHANELKVIIQNENDMVFAEELS
                     NRVNAGCIIQLQPEWSVRNQIMPYLVQYVIDHPKWRLSVQTHKYIKIP"
     gene            3635..5563
                     /locus_tag="PKY63_00960"
     CDS             3635..5563
                     /locus_tag="PKY63_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99210.1"
                     /translation="MKNILIFGLFLLMTVCMQAQDRPLTSKNKKAIKEYYAGVDFLDR
                     FVYDDAISSFEKAILYDPEFIEPYILLGTLYEEKKEYTKAIEYYEKSLTLDPEFFPSN
                     FYNVGLLEIKTGQYEKALNNFQTFLKYNPKPGPKKQAEQSIRICQFAIEEIKNPKPFE
                     PKPLGTGVNTENSEYMPALTADESTIIFTRLIKSTSAESYSEYQEDFFTSKLIDGQWS
                     TAIPLSKKINSPLNEGAHCISPDGKILYFTACGRNNNKSCDIYMSKLRHGEWDYPLIV
                     PKINTEAWESQPSISADGKTMYFVSGRYNKNYDLFVSTYNDSTGYWGEPERLPDNINS
                     SGVEQTPFIHHDGITLYFTSNGHIGMGGQDIFYTKKIDDTTWSDPVNIGYPINTHADE
                     ACLHISASGETAYFATGNLDVEKQEVWNMDIYSFDLYREARPIPVTFLKGIVSDINTK
                     QTLQASFELTDLKSGKIVAKSTSDENGAFLLCIPTGMDYMLNVSAEKYLFYSDFFSLS
                     GTHTKTDPLIKNISLQPIGSDAVVVLNNIFFDTDQYDLKQESLIELDKVKKMMLSNPK
                     MKIEIRGHTDNTGNKQHNLTLSENRAKAVVMYLCSQGIAKERLVYKGYGDSMPRATNE
                     TAEGRALNRRTEFKVISN"
     gene            5568..6275
                     /locus_tag="PKY63_00965"
     CDS             5568..6275
                     /locus_tag="PKY63_00965"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPE99211.1"
                     /translation="MINKQKIEKWNQETAKLNPEQIIDYAFRNIDGPVTMATSLSIED
                     QYLLYLILRNGHECSFFTLDTGRLPQETYDTIAATEQHFGIRIHSYFPDRHEVEAFVN
                     QNGVNCFYESYELRKTCCRIRKTIPMDRALAGKQAWFTGLRREQSVTRINIPIFDLNE
                     NTGIIKISPLADMFEKEVWTEIEENNIPFNPLQKKGYRSLGCLPCTRPISDDDDFRAG
                     RWWWENPENKECGIHKK"
     gene            6609..8063
                     /locus_tag="PKY63_00970"
     CDS             6609..8063
                     /locus_tag="PKY63_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPE99212.1"
                     /translation="MNPNLILLVFVIYTLLLFFITWLTSRKANNETYFIGNRRSIWYV
                     VAYGMIGASLSGVTFMSVPGMVEQHGFAYFMVVIGYFIGYQVVAFVLLPLYYKLNLTS
                     IYTYLDQRFGNSSYKTGSLLFILSRSLGASMRMFLVVLILQNFLFDSLGIPFALTAII
                     FISLVLLYTFQGGIKTIVWTDTLQTTFMLGALIFTLFAIASQLNISIGDLFSTIADSK
                     YSNVIVNDFNSKDHWLKQLLSGIFITITMTGLDQEMMQKNLSCRNLRDAQKNMVTFSF
                     IVVGVTLLFLVLGAALYIYTDTLNIVRPALADDLFATVSFNYLPAIAGFVFLIGLISA
                     AYPSADGAITSLTTSFSLDILRINRKEYSEKKQKRIRLLVHFSFALLLLGLIILFSTI
                     NDRSIIDKLFTIAGYTYGPLLGLFAFGLYTSRPIRDKLVFIPVIVSPVLCYLLAANSK
                     EWFGGYQVGFELLIINGALTFVGLWGLSRGSVRS"
     gene            8266..9567
                     /locus_tag="PKY63_00975"
     CDS             8266..9567
                     /locus_tag="PKY63_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99213.1"
                     /translation="MERKVYKKLLDWKKTRKRKPLLLQGARQVGKTYLLKEFGKKEYS
                     KVFYLNFESDKRLDSLFRTSIEPAAIISKLRIFLNEDFDVDTTLLIFDEIQSSERALS
                     SLKYFNEDENEYHIAAAGSLLGIAMNRKEYSFPVGQTQMMHVFPMDFEEFLMAVGKSK
                     LVEEIRKCYENDAFCAFHEMALSLFHDYLITGGMPEVVAEFVKNNDHLKAARIQQQIL
                     DSYIADMAKYATPEEVSKIIASYNSLPAQLAKENRKFQYKVVQKGGYAGMFGAAIDWL
                     SSAGLVYKCNRVTVPNEPLSVYSDMTGFKLYMNDAGLLVKKSGMPLREILIENKHTGS
                     FIGSVVENFVAAAFQSLGIPLFYWDSGNTAEVDFLIQGQSGVIPVEVKSVTNTRSRSI
                     DVFRKKYNSSKIIRLSEKNFGFDNQIKSVPLYAAFCMDSEL"
     gene            9648..11405
                     /locus_tag="PKY63_00980"
     CDS             9648..11405
                     /locus_tag="PKY63_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPE99214.1"
                     /translation="MEIKRLFDLLDRYKEKFNKPVLFGGKVDGIWQRYSTQQHIVNST
                     NISYGLMQLGIQPGDKIISITTNRPEWNFLDMGIMMCGAVHVPIYPNISSGEYKHIIN
                     HSEAKMVFVAGEDMYSRIKDILAECKNIESVYTFRNLHGIRHLNELMEMGRQNPQSEK
                     LQSIKDSIKEDDVATIIYTSGTTGIPKGVMLTHKNLLSNVEAIYTIPAQNPALVTLSF
                     LPLCHVLERIMNYMYQYNGYTVYYVDNIALISDAMRETNPSIVCCVPRVLESIHDKIM
                     ATGRKLTGYERFFFYWAVNIGYRYRIEGNNIFYRMQLWLLRKLVFKKWKQALGGKLDI
                     IVSGGAALQERLARIFWAAGFRVLEGYGLTETSPVISVSNFEKNGIEFGTVGPPLKNV
                     EVRIADDGEIQCKGPNVMKGYFKDAGQTKEVLSEDGWFKTGDIGFITQHGHVKITDRK
                     KEIFKTAAGKYISPQLIENKLKESPFIDNAMVVGENQKYPAAIISLNFFHVRSWCEIK
                     KIPFTTNEDILQNPVIKKRIAVEIEKVNLKLGEHERIVKFEFTGNTWSVDTGELTPTL
                     KLRRKIISEKYSSLLSKLF"
     gene            11416..12441
                     /locus_tag="PKY63_00985"
     CDS             11416..12441
                     /locus_tag="PKY63_00985"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99215.1"
                     /translation="MKKFILLLSIISVVMLNSCKKDQNAEIKVLTEHIEYDVLINNDG
                     KMDPVMNHVNEDVRVEFVHFLFEQLKAGKAFNDSGASIDSKAVLMLIREMFPNADTSV
                     IDPEVYYKINTAKINKLRFREKWIYNSENFKIEKTVLAVAPLVELADTLGYVFKAEPL
                     FWVQCDTAKELKEVTVLSTNIISDAFVHNQLEMILYMDSTPADYYCNLNDSAKTAYFD
                     ELVKSVIDKKINAYNFFFNPLEEADMKVLKGYADTLSDFDENNNPVKTVIEHKISAKE
                     FGRIKFAERWEYSAYPFVFRKTVMALNPSVIVIDPQYDVVKGFKPLFWTVYDEKYLQE
                     MKGKILQ"
     gene            complement(12509..14827)
                     /locus_tag="PKY63_00990"
     CDS             complement(12509..14827)
                     /locus_tag="PKY63_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPE99216.1"
                     /translation="MKTGIILFLFVIMAWTANSQEIYQRAKVSGSADFLNTIGSLGIP
                     VEGEIKKGEYLICDFSQTQIQKMRDAGLTVEVIISDVQQHYIKQNQSMNPRNMRNTGC
                     FEIPDYAVPAHFNHGSMGGFLTLAELYAELDEMRAVFPNLISARQIVDTIHTIGGRQL
                     YYVKISDNPDVDETEPEVMFLALNHAREPMAMQQEIFFMWYLMENYATNPEIQYLLQN
                     TEIYFIPCVNPDGYEYNRTTNPSGGGMWRKNRRNNGDGSYGVDLNRNYGYQWGFDDIG
                     SSPFGTDETYRGDSAFSEAETQAVRNFTLAHDIQLIQDYHTYSNVILYSWGYADLPTP
                     DDILYRTYSSYLASENSFAYGTPMQCIGYNANGGSFDWYYGEQTEKDKIIAWGPEVGD
                     PADGFWPAEALIDDICKLYVSENLYMVRFACKYAEVKDLSELCSPWIIPFSIQRLGMD
                     TPAVYTVSLTALTPNVMVMTSPITFTDLALLETRLDTFDISLAIPYAQGDEIRLELSV
                     NNGVYTTRDTLVKYSGMSSVLFSDNATNTTNWAGGWSTTGSDFVSSPYSFTDSPGGMY
                     SNNTNSSMTLTLPLGSVLAEKAWIEFYAKWDIEEGYDYCVCEISTDNGATWIPQCGQY
                     TTRGSADQLQDEPLYDGTQSTWVKEYIDLTDYLYADRKLRFRLVSDSYEQRDGFYFDD
                     FTLNIIQAVGIDETELNILKLHPNPATNELLLSNVAQGDYFISDISGNIVQQGICEGG
                     ALNISNLAQGLYFITVIENGTMLRAKFVKAEY"
     gene            complement(14940..16085)
                     /locus_tag="PKY63_00995"
     CDS             complement(14940..16085)
                     /locus_tag="PKY63_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPE99217.1"
                     /translation="MYIHTDIFAAQSPKKAGQPCGDAWGVYRNENATTLVLADGLGSG
                     IKANIAANLCVSRILTLIKMGLSIRETFNRVSATMDSAWGTVDPFSVFTIVQILNNGQ
                     TTILGYEMPPPLIISRAYAQVLHDRVYTREKAVVHESSCMIEKGEGLMLMSDGITQAG
                     IGKLFTNGWESEGVCSFLQSHLTGKRISGNDLAQLVHNQARSYWPQNKGDDCSVVVAV
                     NRRGVVVNLLSGPPLHATDDENFVHAFYNEPGIHIISGGSTSKMAARVTGHSIDVVDD
                     GDALTPPSFKMKGTDLITEGMVTLNQVCNLLDEDLAALPTGSPAYEMTYFLKMADKVN
                     IWMGMADNVSEDRIEFRQQGLLSRGSIIKRISEKLLKQGKLVVMNSL"
     gene            complement(16114..17835)
                     /locus_tag="PKY63_01000"
     CDS             complement(16114..17835)
                     /locus_tag="PKY63_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPE99218.1"
                     /translation="MNNNPMQTDNISVVYTSRALCRDCYRCVRVCPVNAIRMKDGQAY
                     VVAERCISCGTCITVCPQKAKTYRTEYGRVLQMMEDGERLAISMAPSFAAYYPEWRRK
                     RLPSALRMLGFSVVGETATGAWHTAQATAEFIEHNPDQPVICTACPAAVNYITSKMPD
                     LMPYLAPVASPMVAHARMIKKTYPGIKFVFAGPCIAKKDEAKWKSTENDIDAVITFEE
                     LDELFRLRNISLDACEESTFNDPVNGLARVFPLESGLLKTGNIADSMFGRVVMALSGA
                     DEIRELLDDLHRNPTNCVIEPLFCKNGCVNGPAMQHNCNSFEARRMVLEYASAYPGEE
                     AEKSSLFKDTDVVFSLASGNDHPVFSEEQIREVLSKTGKLIPADELNCTACGYKSCRD
                     RAIAVLEGMAEPEMCMPYMRRLAEQKADTIIERDPNGFVLLNENLEIIRMNQAFKHMF
                     SCSDSLVGRKISYLIDPKQFENLIAGKEDATREYNHYPNYNLMCHTVAYRLPEEEQMV
                     GLFVDVTDSKSSKDKLNALRSETVDRAQELIDHQIDMAQELARFLGENTARGEVLMKN
                     LIDSINK"
     gene            complement(17944..19995)
                     /locus_tag="PKY63_01005"
     CDS             complement(17944..19995)
                     /locus_tag="PKY63_01005"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPE99219.1"
                     /translation="METKGYITIDGTDVPVENEKNLLEVIRKAGIEMPTFCYHSHLSV
                     YGACRLCIADIDGMGIQATCSIKPKPGMKVQTNTAEVRKIRKVNLELLLANHKIDCPS
                     CSRSNNCKLQDLARRLNVNEIRFKKTEMDLPVDRESHSIIRDPNKCVLCGDCVRACKE
                     MQSVGAIDFINRGARTIVGPAFDKSLNDVECVFCGQCVSACPVGALVPKSEVEAVWDV
                     IHDEKKVVVAQIAPAVRVALGEGFGMEAGAISTGQIVAALKHIGFNQVYDTSFAADLT
                     VLEEATEFIARKLKGEKLPQFTSCCPGWVKFAEQYYPQLLPNLSSCKSPQQMFGSVAK
                     EILPGILGVKPENLVVVSIMPCVAKKFEARREEFIHDGMQDVDYVLSTDELGRMIHET
                     GINFRTIKPESFDLPLGFKTGAGVIFGNTGGVSEAVLRFAYEKLTGETLIDTDIRQVR
                     GEDGIRTVEMTLGKIKLKLGIAHTLGKARQICDKVVAGDSGYDLIEVMACPGGCIAGG
                     GQPVSFDPDFRQKRTQGLYNADKQLELHKSQDNPYIKELYKSFLGEPGSDKAHHLLHT
                     HYKSRKRINDQVINIRDTEHPKIEVYVCVGTNCYMKGAHKLLHQLADYVSENKLEDIV
                     GFEGHEEMVDVKATFCFERCQNGPVMRVNDKILEHATFEKAKQLLDQEIKRMGLQLQN
                     E"
     gene            complement(20016..21923)
                     /locus_tag="PKY63_01010"
     CDS             complement(20016..21923)
                     /locus_tag="PKY63_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPE99220.1"
                     /translation="MKTMNKATLEKIRDEYKSASGVVSKRIIVCAGTGCIANGSLAVY
                     DALKEAVETLGASLFVELELDRHDCKDKVHLSHSGCQGFCAQGPLVNILPDETMYVKV
                     KPEDALEIVQKTVLANEVIDRLLYVNPANGEKSRGQLDIPFYKLQNRLVLGECGHVDP
                     ENIREYISHSGYFAAEKAYNSMTDEQVCDNILESGLRGRGGGGFPTGRKWHLTRVVES
                     DKKYVICNGDEGDPGAFMDRSLMEGNPHRVIEGMMIAARAINADEGYVYVRLEYPLAV
                     KRMRKAIDDALEAGILGDNVFGSGRSMYIHVMEGAGAFVCGEETALMASIEGKRGMPM
                     PKPPFPAECGLFEKPTVINNVETLAAVPLIIRNGAAWFNQYGTATSKGTKTFALTGHV
                     ANTGLIEVPFGTTLREIVYNIGGGVMDNDGKISSKGFKAVQIGGPSGGCLTAEDIDLP
                     LDYDNLIAKGAMVGSGGLVVMNQESCMVQIARFFMNFTQNESCGKCLPCREGTKQMLA
                     LLDDIIEGNATMKTMDLLLEVGHVVKKASLCGLGKTAPSPVLSTIAKFRDEYVEHIER
                     KHCPTNNCKALMDITIDPDKCVGCTACARKCPVNAISGEKKKAHSIDPALCIKCGECH
                     KACKFNAVMGF"
     gene            complement(21946..22452)
                     /locus_tag="PKY63_01015"
     CDS             complement(21946..22452)
                     /locus_tag="PKY63_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPE99221.1"
                     /translation="MKTEPNKSLRQFQQVHEILEANGNNKARLIPILQQVQDAYRFLP
                     PQVLTYIATALGMPIAEVYGVATFYAHFTLEAKGKYVVKACDGTACHVKGSSKLLDTL
                     YDTLKVTPQKNTTDDMMFTVEAVSCLGACGLAPVMMVNEKVYGEVTPDRCREILKAIM
                     EEESVVKE"
     gene            complement(22460..23143)
                     /locus_tag="PKY63_01020"
     CDS             complement(22460..23143)
                     /locus_tag="PKY63_01020"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPE99222.1"
                     /translation="MEAKSINQKNVPQGIPEPTVRRMPQYLNLIREVSRNGEPNISAP
                     VIGRVLRIDPTQVVKDLAYTGITGKPRVGYVVEELLFALERFLGYHRQHEAFLVGAGF
                     LGSALIQYPGFREAGVNIVAAFDTDPAKIGTEISGIKVYNMNKFSDLVQRLHIPIGII
                     TTPAEAAQSVAEQMVVWGIKAIWNFAPVALRIPENIILQDTHFYANLAVIVNKLEQSE
                     PQTKKGSKK"
     gene            23409..23789
                     /locus_tag="PKY63_01025"
     CDS             23409..23789
                     /locus_tag="PKY63_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPE99223.1"
                     /translation="MDIRDFIQNDRFAKLLGIELVEAAPGHAITQMQITDEHLNAVNI
                     AQGGVLFTLADLALAAASNAHGPVAVSIQSEIKYFKPAGKGLLTAEASEISCSSKLAT
                     YSIDIRNKDGELVAAFQGMVYRNE"
     gene            24005..24727
                     /locus_tag="PKY63_01030"
     CDS             24005..24727
                     /locus_tag="PKY63_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPE99224.1"
                     /translation="MNSIEICFTPALYGHIMTEPPFTVAIVDILRATTSICAAFDNQV
                     KEIIPVGTTEEALEYKNKGYLIAAERNGIKLDFADFGNSPDNFTRDHVRGRSIVYSTT
                     NGTQTIKKITNGNPVVIASFINLKAVAKYIVAKNQNLVIVCAGWKNKFNLEDSVYAGA
                     LTAELLNMGMTMNCDSAQAAVDLWTIAKDNPVAYIQKAMHRERLKKLNLDSILEYCFT
                     PDITNAVPVFDGYKILNATLNE"
     gene            24720..25706
                     /locus_tag="PKY63_01035"
     CDS             24720..25706
                     /locus_tag="PKY63_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HPE99225.1"
                     /translation="MNKFLKSITVIIVLFCLPLGANAWGFFCHRLINKHAIFILPPEM
                     VGFYKKHMDYIVQHSVDPDKRSHAVEGEAEKHYIDIDHYGDKPFEIMPRQWKQAVEKY
                     SEDTLHEYGILPWNIEWMMYRLTDAFKEGDVDKIVNVSANFGHYIGDCHVPLHTTQYY
                     DGRKPYQKGVHALWETRIPELLAINYDFFIGRAGYISKPLDKAWELVEYSHNEMDSVI
                     NMYDSLFLNYDQSSIFVMEERGSVVSKQYSMVFCKLMEKEMEQMVYRKLVMSIYYVAS
                     FWYTAWVNAGQPDLDKLMSKEVSKEHQKEMDELDYLWKNGKPKGRPNPEDEE"
     gene            complement(26210..26614)
                     /gene="mscL"
                     /locus_tag="PKY63_01040"
     CDS             complement(26210..26614)
                     /gene="mscL"
                     /locus_tag="PKY63_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPE99226.1"
                     /translation="MKMFKEFRDFISKGNVLDLAVAVIIGAAFGKIVTSFVNDILMPP
                     IGILMGGVDFKDLSVVLKDAVGDVPAVTLNYGMFVQNVIDFLIIAFCVFVIVKVANNL
                     SKKNEEAPAAPPAPTKDQELLTEIRDLLKERK"
     gene            complement(26628..27581)
                     /locus_tag="PKY63_01045"
     CDS             complement(26628..27581)
                     /locus_tag="PKY63_01045"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPE99227.1"
                     /translation="MKHFFIVAFMLISATMFAQVTDKESDLRKLNADTTEGWKKGGMF
                     TVAFSQVSLSNWAAGGENSFSGNSIVNSFANYKKGKISWDNTLDLGYGIMKQGEQEVR
                     KTDDKIDFSSKFGRKLSSNSFAAALLNFKTQFMPGYNYPNDSVAISRIMAPGYLLFAA
                     GYDFKKGDWLSLFLAPVTGKMTFVLDETLANAGAFGVEPAVINPLDSTILTAGKHMRT
                     EFGGYFKGAFTKDIMKNVKLSSKLELFTNYLKNPQNIDVYWETNIGMKVNKYITVTIG
                     TTLIYDDDIDIEIKDEAGIVIGKGPRTQFKEVFGLGFSYKF"
     gene            complement(27655..29115)
                     /locus_tag="PKY63_01050"
     CDS             complement(27655..29115)
                     /locus_tag="PKY63_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99228.1"
                     /translation="MKQVTGLLLISLLFFQMMSAQTVENKWSIGFFGGKNEYTGDYGN
                     AIGDFSKAFYGFGAMQLNRYLSPSFDLGLSASYGDYGYFKSSAERFLGKKLDPALLLS
                     LKLNNDILFPAKWKFSPSLIAGAGLAYYNGNCISVSGVDFIVPLGLGLKYQISPKIAV
                     QYIGTYQLTFNDDRDFAVADMNDCYVKHSVGIVIGFGAPCDSDKDGVKDKFDKCLNSP
                     AGSVVNAEGCPADSDGDGVMDNVDECPDVAGLSNLAGCPDRDKDGIADKNDKCPDVAG
                     ITKFEGCPDTDNDGIQDSEDNCPSVAGIEKFQGCPDTDNDGIVDSEDRCPKVAGTAEF
                     KGCPDRDGDKVADIDDKCPDVPGVVANKGCPEVKEEVKKIFTQALTGIQFETGKDIIR
                     PTSFTILNNVVKVMQDNPEYLLEINGHTDNVGDDAANLDLSQRRANAVKKYLADKGID
                     AARMTATGYGETVPVADNATVAGRAKNRRVEFKVNF"
     gene            complement(30418..31275)
                     /locus_tag="PKY63_01055"
                     /pseudo
     CDS             complement(30418..31275)
                     /locus_tag="PKY63_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(31327..32421)
                     /locus_tag="PKY63_01060"
     CDS             complement(31327..32421)
                     /locus_tag="PKY63_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99229.1"
                     /translation="MSAILLNAQEQKPLRMHLVDGTSHKISWVVRNGHWVKVVTTEGK
                     HSFGAIKLHSENLFLVGNDSINPAEVFTLKYCTPGFIALCSTPLLLIQYQFFYITLGA
                     FIYEEFTTRVKGDDFVVINRMRKKETTALLQSDSIRQYLRQDTNKTPEKKENAKKLAK
                     DSIRFEKRLLRSEDPSEFCFIGTDLEKLPVPNFTFNFDNYFRPSWGIALQAGYKPLSP
                     NGYVYSVRRVNEPDCISSHIMFRLNPVFTFTNRSDSSCFYFIGPSLYFKQIMGDSIKV
                     YQSNYISTQSRPSTYWHDKTVTGIGLRMGYSSKRRVGLSFSCELSLRQFSEHNDVIRY
                     DESTFQSDDGGMYFNIDFSVALRLKLRKHH"
     gene            complement(32521..32889)
                     /locus_tag="PKY63_01065"
     CDS             complement(32521..32889)
                     /locus_tag="PKY63_01065"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPE99230.1"
                     /translation="MELSGKIIQVLEAVSGQGQNGTWKRQDFILEVPGQYPKKICITI
                     FGDKVEQFNVKQGEEVRVEADIESREYNGRWYTSVKAWKVEKAGGDMQDRMPDYDVAQ
                     NAPTSGGIDLSVNEGDDLPF"
     gene            complement(32995..34254)
                     /locus_tag="PKY63_01070"
     CDS             complement(32995..34254)
                     /locus_tag="PKY63_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPE99231.1"
                     /translation="MENLIFILIIAFLLFDSILDTWLSVLNSRNWPQSVPERIASVFT
                     QEKFLKARSYSMDRSRLSHIVSFFQLAIILAMLFAGGFGWIDNLAAGIWNHSIGHSII
                     FFGILLLSADILTLPFQWYSVFTIEQKYGFNKSTPAIFIGDKFKSWIMSAILGGGIFW
                     LLAEFYYSISDYFWLLAWGVLTLFSLLMTMFYSNIIVPLFNKQIPLKAGELRDAIEFF
                     AKTNGFNLKNIYVINGSKRSTKSNAYFTGIGPKKRIVLYDTLIEKHTSEEIVAVLAHE
                     IGHYKMKHVTTGFFMSTVLNLGIMYLLNLFLSKPEFAGALGMEPSLHASLLVFALLFS
                     PVSTIMGIVSNVISRRHEYAADRFAWEHTHSPALADALIKLSVDNLSNPTPHPYYVFM
                     NYSHPPVLERLKALDGYTVTDCKSAPE"
     gene            complement(34266..34955)
                     /locus_tag="PKY63_01075"
     CDS             complement(34266..34955)
                     /locus_tag="PKY63_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99232.1"
                     /translation="MKNLLILSMLSLFFFVSCGNKPDQEKVASMLDSLKEITAVAPSD
                     SDQFGFKTGVIVYTSSTMGITQEITMWFDDFGRKTLSEIKSTVLGQKVHQLNIVKDSM
                     VYNIDMVLKVGSRMTMEQDSSEANYNYRTLSADDMKRYNIVFEGSEKFLEKECKIYNQ
                     KQNVNGQEIDMKVWIWEGIPLKSVSSVSGIEVSMEAVKMKVNVEIPSQKFDVPAGVQI
                     EEAADTTSLPS"
     gene            complement(34968..35558)
                     /locus_tag="PKY63_01080"
     CDS             complement(34968..35558)
                     /locus_tag="PKY63_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPE99233.1"
                     /translation="MNYLFHLVLSGDNPDIIIGNYIADHIRGTIIMAELPNPVLKGVL
                     LHRAIDAYSDMHPAVRHSVALLKPSHARYATVAVDILFDHFLIKNWKQLVKTPMIKKI
                     NAFYALLHEKMDTLPPKSQKVSHKLMQDNWFGKYETFDGLNEIFIGVTKRTHFANALP
                     DTVEYLVKYYNEMDSDFKIFARDIFSFVSLHTKYRL"
     gene            35645..37252
                     /locus_tag="PKY63_01085"
     CDS             35645..37252
                     /locus_tag="PKY63_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE99234.1"
                     /translation="MQKQLLLGDEAIAVGAIDAGISGIYAYPGTPSTEITEYVHSSKE
                     AQQKGILASWGANEKTAMETALGMSYAGKRAMVCMKHVGLNVAADPFMNAAITGVNGG
                     LIVVSADDPSMHSSQNEQDSRIYGKFANVPILEPSNQQEAYDMVHYGFELSEKFKVPV
                     LMRITTRLAHSRAGVGRIEGRKQNDMSLPSDLRQFVLLPAIARKRYNMLLGNQSGFRS
                     ESASAGFNQLIDGPDKSMGIIACGIAYNYLMENFPQGCPFPVLKIGQYPLPVNLVENL
                     ADTTEQIMVLEDGYPMVEELLAGLLQRGIKVSGRFDERIPRTGELNPNIVARAFGMAD
                     THGLPSPEIVVGRPPSMCVGCPHIDSYNAMNEALLDYSKGRVFSDIGCYTLGALPPFE
                     SINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFCHSGMTGLLDAVIAKSPITVVIL
                     DNSTTGMTGGQNSAAFGRVKDIVTGLGVEPEHIRILKPLKKNHEENTQVFKEELAYNG
                     VSVIIPTRECIQTLNRRMREKFKNKAN"
     gene            37265..37852
                     /locus_tag="PKY63_01090"
     CDS             37265..37852
                     /locus_tag="PKY63_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPE99235.1"
                     /translation="MKKDIILSGVGGQGILSIAATIGLAAVDKGWYLKQAEVHGMSQR
                     GGDVQSHLRVSENVIHSDLIPMGQADMILSVEPMESLRYLPYLHKDGWLVTNSKSFIN
                     ISNYPDIEKVFAEIKKIKNHILIDADEIAAGLNAPKSANMVMLGAAFAFLGIERNYFE
                     NAIRNLFGRKGEEVVNININAFNAGVEFAQQYIKK"
     gene            38084..38515
                     /locus_tag="PKY63_01095"
     CDS             38084..38515
                     /locus_tag="PKY63_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE99236.1"
                     /translation="MKKNNEKKMVELNEELENIEVMQETADKNENSGHREMNVVELCG
                     YVGFDPEVQDFASGKKKIRFKLATHRTLRTDGEGEGQKVTNWFPVVVWNKTADEALTW
                     LRKGNKVKIKGYLNYRTWEDKDGNKRNLYETVATELSAIPA"
     gene            38601..39452
                     /gene="dinD"
                     /locus_tag="PKY63_01100"
     CDS             38601..39452
                     /gene="dinD"
                     /locus_tag="PKY63_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HPE99237.1"
                     /translation="MKKEEITELFNKFENACYVYQDIECWSARELMEILGYTKWQNFE
                     KIIDKAIQSLENAGETALDHFTDVSNMVQIGSGGVRALSDIALTRYACYVVAQNGDPN
                     KPEIAFAQTYFAVQTRKQELVEQRLLDVARVNARAKLTKSEKKLSEILFSKGIDEKGF
                     AHIRSQGDKALFGGKATLEMKKKLLVPDSRPMADFLPTLLIKAKDFATELTSHNVVEK
                     DLEECCAITEEHITNNTSVRKILLKRGVKPENLPPGEDIKKVQRRLDTETKKVCANTK
                     KIKPRKK"
     gene            complement(39498..40802)
                     /locus_tag="PKY63_01105"
     CDS             complement(39498..40802)
                     /locus_tag="PKY63_01105"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE99238.1"
                     /translation="MILPWSEIKNYLFRATFTFRALRSKNYAIYLSGMLFSVLGTWIQ
                     IVATGWLVYRLTDSAFMLGIVTFAGQIPSLIVTPIAGVYADRMNRRKVILFTQYASMF
                     LSLTLAILVLSDHIEVWHIIALAALSGIVNSIDTPFRHAFIRDLVEDTSQMSNAIALN
                     SMLFNTARFVGPMIGGILIAIIGEGWCFAINAVSFTAVIASLYAVRVGFIPKENNTNT
                     IIEDLKDGIKYSFGFAPIRYMLILVISTGFFILPFQSFLPVFARDVLNGDSSLYGVLT
                     GSYGAGALIGAFFLASRKSLKSFPVYIMIAAFVFSGGLALFSCSTVTALSMALLVLCG
                     WAMIAQYVSVNTLLQTIAKPDMTGRVISFYGMSFMTVTPLGAILIGTLANSFDIQDIF
                     ITFSLLSLIAGLIYSSQKSRIQKELSRVLKAESEVGNSNDHI"
     gene            complement(41137..43095)
                     /locus_tag="PKY63_01110"
     CDS             complement(41137..43095)
                     /locus_tag="PKY63_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HPE99239.1"
                     /translation="MTENIRNIAILAHADAGKTSITELMLHHSGAIKNPGSVDDGTTH
                     TDFLAVERERGISVRSAHTTFLWKDIRINLVDTPGHVDFSADVERILIAVDGAVLVIS
                     AAEGIQAHTGTLWNALRARNIPVILFLNKLDRLGADIENVLMDIRKEWHLEPLCMQSV
                     QNIASTDVVITDLFSKDHIAENIAETIVAGDEKLMDAYLEGVMPDFEILEKQLADMVS
                     RSEIVPMLFGSARLNIGIDQLLDVMIKFLPAPNENAVDGLRARVFGISYDKTMGKVAH
                     VRLFGGSISNRELIRNATQSVDEKVVQLRRMSGMRFEDLQQVVAGDIAGVCGFSAASV
                     GDWLGESVLDTSNLKPLQMPLLTVRVKALNEKDYSPLSAALQQLYSEDPSLNFEWLRD
                     EKEMNISVMGWIQMEVLERILLDRFGFAATFEQPTVIYKETPSSIAEGFVRYWMPKPC
                     WAILKFRIEPGEPGSGVVYRSEVRVDDVAQKYQNEVEQTIPAALKQGIKGWEVTDLRI
                     TLIEGEDHEIHSRPGDFIIATPMGIMNGLVNSGTTLLEPFVTFRIKATEDLLGLITSD
                     ITKMRGTFDSPEMDNGKITLTGFLPVSTSHDYAVKLSSRSGGKAEISTQFAGYRPCAD
                     ELGVVRPYKGISPLDEAKWILKARGAMQ"
     gene            complement(43092..44273)
                     /locus_tag="PKY63_01115"
     CDS             complement(43092..44273)
                     /locus_tag="PKY63_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99240.1"
                     /translation="MKSTPFIYGTTVSINSFTDREKDAEKLYSNLTEGVNTSIISPRR
                     WGKSSLVEKVLHVIKTKNRNTITVQIDLFSASSAEDFLELFAREVIMASSTKWQDWVK
                     SAKEFFSRLIPQITIGVDPNTDFRIGFNWTELNKFPDEVLNLPERIAVKQGKQYIICL
                     DEFQNLASFAGFASFEKKMRSVWQRQKNVTYCLYGSKRHMMTEIFNNPSKPFYRFGDI
                     MLLNKIPRKNWVTFIVNGFKKSGKSISAEMAEKIPFLMNDHSWYVQQLSHYTWQKTEK
                     SAGIEELSAALTELINANSPLYQKEVESISVTQLNFLKAVVCNETQMTSASVMQKYRL
                     GTPRNVQKNRTILVENDIIDLENGKHVFLDPAFELWFRMQYFGEKYLNNPALTTQKTK
                     R"
     gene            45002..46501
                     /gene="hutH"
                     /locus_tag="PKY63_01120"
     CDS             45002..46501
                     /gene="hutH"
                     /locus_tag="PKY63_01120"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPE99241.1"
                     /translation="MKKQTLIIKSEIRFADIEPIMLGHVQVELSAAALKKINICRQYL
                     NQKIEKSDAPVYGINTGFGSLCNTSVGKKDLSKLQENLVLSHACGLGNEVRPEIVRLM
                     MVLKIQALSLGHSGVQTDTVQLLADMYNNDILPVVYEQGSLGASGDLAPLAHMCLPLI
                     GKGDVIYQGNRMSSKQAFKTVGLKTVQLQSKEGLALLNGTQFMSAHGAYALVMSKNLM
                     QWANHTAALSLEAFDGRTDPFHPMIQSVRPHPGQMQVANAINKILKGSKIAARKKQQV
                     QDPYSFRCIPQVHGATWDTIQYVNEVFKREINSVTDNPTVFPDTDLIISAGNFHGQPL
                     ALAFDFLGIAMSELANISERRIYQLINGKHGLPNYLVANPGLNSGFMIPQYTAASIVS
                     QNKQLATPASVDSIESSQGQEDHVSMGANAATKTIRILENVEKVLAIELMAAAQALEF
                     RRPETSSSYMEKLIAEYRKEVPFIQQDDYMSVHMHRSIEFMRTFKLKAE"
     assembly_gap    46534..46633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(47794..48396)
                     /locus_tag="PKY63_01125"
     CDS             complement(47794..48396)
                     /locus_tag="PKY63_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99242.1"
                     /translation="MRNLLILLLLLSGFSALQAQVTEISIADDVCDSLSAIDFSKSKE
                     EIIRDSKPLLTLSLFSHAEDIISIGKNYKAQNPDLSETDAIKHVNQEIIFQLMKKCVP
                     FQRMTMFNCDSVPELSETTVLVGEAFTAMLIEKTKNQPIDQNIVDNCVVDVIDNYETK
                     IIDSFGEKFHPDFLAEFNAYLLTRCEPYMHWTAELISLQY"
     gene            complement(48428..49774)
                     /locus_tag="PKY63_01130"
     CDS             complement(48428..49774)
                     /locus_tag="PKY63_01130"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPE99243.1"
                     /translation="MESYHIFNARIVNESRIFEGEVFISGGLIKKINEGKNSKTPAGY
                     TAIDAKGQYLLPGVIDDQVHFRQPGMEEKATIYSESRAAVAGGVTSYMEMPNTKPPTL
                     TQDLLEDKYKIASKDSLANYSFYMGVSNDNIEEVLKTPLDTVCGVKIFLGSSTGNLLV
                     DNPKTIEQLFAETPHIVAAHCEDDEIIRRNAESFKDFFGDEISASVHPSIRSAEACFK
                     SSSYAVALARKKKTRLHVFHVSTAKELELFDAGMPLEKKRITAEVCVHHLWFTNDYYR
                     THDNLVKWNPAIKTAEDRKALREGLKAGLLDVIATDHAPHLLAEKMKPYFDAPSGGPM
                     VQHSLQAMIAMSESGLWSLADVVKYMCHNPAILFNVSRRGFIREGYYADLVLVDTSKK
                     YKITKSNILYKCGWSPLEGETLPSTIAKTFVNGHLVWDNGKIVGTKVGKRLGFERD"
     gene            complement(49774..50517)
                     /locus_tag="PKY63_01135"
     CDS             complement(49774..50517)
                     /locus_tag="PKY63_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPE99244.1"
                     /translation="MNAVSDSLVIIPTYNEKENIENIIRKVFSLEKDFHILIIEDNSP
                     DGTADIVKGLIPEFNDRLHIEERKGKLGLGTAYIHGFKWALAHGYEYIFEMDADFSHN
                     PDDLIRLYKACHDDGYDMSVGSRYITGVNVVNWPMGRVLLSYYASAYVRFITRMKVRD
                     TTAGFVCYTRKVLDAIRFEKISFLGYAFQIEMKYTVHKLGFSIKEVPIIFTDRTLGQS
                     KMTKGIIREAFFGVIQLRLRNTKRRHKKQ"
     gene            50601..51674
                     /gene="lpxK"
                     /locus_tag="PKY63_01140"
     CDS             50601..51674
                     /gene="lpxK"
                     /locus_tag="PKY63_01140"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPE99245.1"
                     /translation="MGLRFLLFPFQWGYILLISLRKLLYAVGIKRRRTFNMPIICIGN
                     LNAGGSGKTPHTKYFAQLLQRNHYKIAIILRGYKRSTRGFIHCNTDHTVAEIGDEAYE
                     YVRNLDEKTNIFVCERRRKGIKEIMNKFPETDVILMDDGFQHLAVKAGMYILLTDYLQ
                     PYWKDHVLPIGKLRECKAAKKRAHVIMVTKTPKVFSPLVKRDLIEKIRPRYHQHVAFS
                     YLDYGNPVPVYAEGDQRFTAPENIYSAFLLCGIANPYPLEEFLKRKCIELYSHKYPDH
                     HAYKLKEIEKLAFDFNNHMMKNKVIFTTEKDVSRLMLPEIKEKLVDLPFFYIPVTIGI
                     HESKEFNLDKLIIDYVGKNCKNS"
     gene            51646..52458
                     /locus_tag="PKY63_01145"
     CDS             51646..52458
                     /locus_tag="PKY63_01145"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPE99246.1"
                     /translation="MLEKIAKTADFIRSKTKLRPQIAIVLGTGLGGLVNEIEIIDSIS
                     YSEIPNFPVSTVKGHQGRLIFGRLSGKDVIAMQGRFHFYEGYTMQEVTFPFRVFKNLG
                     IETLLLSNAAGGMNKNFKVGDIMFINDHINLMGTNPLIGKNYDELGPRFPDFSEPYDH
                     KLLETALGAAKDLSIPVHQGVYVAMSGPTFETPAEYRYVRIIGGDAVGMSTVPEAIVA
                     RHSSMKVFAVSIITDLGGMDEVVAVSHEEVIEAANKAEPKMTAIIKELLKRI"
     gene            complement(52505..52993)
                     /locus_tag="PKY63_01150"
     CDS             complement(52505..52993)
                     /locus_tag="PKY63_01150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPE99247.1"
                     /translation="MEAIEHIVFDWGDTLMRDFPGTPGPMYQWERIEVFPNAAEVLEK
                     LSAKYVLSVASNAGESDTADMRRALVMGKIDHYFKNHYTSKDLGVCKPDPEFFLRICM
                     EADFCIDKSVLIGNDYKKDIVGAKTAGMKTILVNHAGAEGPFELADYVVSELKELLKI
                     LL"
     gene            complement(53002..54129)
                     /gene="amrS"
                     /locus_tag="PKY63_01155"
     CDS             complement(53002..54129)
                     /gene="amrS"
                     /locus_tag="PKY63_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPE99248.1"
                     /translation="MEKRDFIKNLFVMGVAAVAMPRLYASESMSNFKSIRHNGMIKAR
                     YFEELPDGSVKCRLCPHACILKNGQSGICRTRINVGGVLYTMAYGNPVAVHIDPIEKK
                     PLYHFLPSSKVLSFGTAGCNFRCLNCQNADISQAAPNDVSALDYSPEKVVKTAREHSV
                     DGIAFTYTEPTVFFEYMYDTAFLAQKAGIKTLMISNGYINQEPLKDLIGVMDAFNIDL
                     KSFNDSTYKTLCNGSLQPVLDTIKTIRDSGKWLEITNLMVTAYTDKTDEFSQMTSWLI
                     ENDFAEVPLHISRFFPAHKLLDSHPTNPAVIEQAFHMATSAGMKYVYTGNIRNEAHEN
                     TQCPDCGTLLVKREGYITKVSGMTENKCNKCGRIIPGVWIK"
     gene            54195..55685
                     /gene="amrB"
                     /locus_tag="PKY63_01160"
     CDS             54195..55685
                     /gene="amrB"
                     /locus_tag="PKY63_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPE99249.1"
                     /translation="MMKRMLILLTIMSIFSDTGAQKNLPIRKAAVAGQFYPADAEALE
                     KYFEKEFNQITIAPNDKKVRAIIVPHAGYMYSGRTAAWAFATLREKNTYKNVFIIGSS
                     HIAAFDGASVFHGAAFETPLGQAEINTEITGTLQQNNIFKFPQQYQTEDHVLEVEIPF
                     LQYILEPGFKIIPITIGTKSTYALDKITDALKPYFTPDNLFVISTDLSHYPSYSDAVK
                     ADSAVVQSILSGNPQTFDAVVRSTENAGIKNYVTAICGYSAVYILMSLADDKDGFSYE
                     KLKYSNSGDVSGDFDRVVGYAAIAVRECDLKETLQKKDTFTISENDKKIMFNIARNSI
                     ESKLNDNVHTVSDTLSDMLNKKCGVFVTLHLNNKLRGCIGTFRQDRELAINIHEMALA
                     AAFSDPRFDPLTKEEYKNIELEISVLTPMQKIDSVNQIVLGKHGIYIKRGNMTGTFLP
                     QVATETGWTLEEFLGHCSRDKAGIGWEGWKDEETEIFIYEAIILNE"
     gene            complement(55741..56391)
                     /locus_tag="PKY63_01165"
     CDS             complement(55741..56391)
                     /locus_tag="PKY63_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99250.1"
                     /translation="MNQTFFSLVFLMFFFIGCSSTTKKDNEKSIEKVVVNEATIMLHQ
                     NSEGEKNFPLKDTLRIPLTDVTTSSGSSTSLILKFEENDGGRVNSLDLFLPAAPQLKW
                     HTNHDGSFELYETKLPYSLKGDNYVPYKDALRDSEIKASAYLQSIIKSPEGSAFLYFR
                     FDEISILSFDFKDEKANMEMNLKLVSEQLTDPVYGSYNADVHFVIKDFASSMMMVD"
     gene            complement(56398..58980)
                     /locus_tag="PKY63_01170"
     CDS             complement(56398..58980)
                     /locus_tag="PKY63_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPE99251.1"
                     /translation="MKQCPNCHAQLNDSAKFCTNCGSKIVIAEKLPKKYVLTKCACGA
                     DLKPGAKFCTICGKKIELSAKNSEVIHENRCSCGAELKPGSKFCTVCGKKVEDVADKP
                     AEIKENLCSCGAELKPGAKFCTVCGKAVIAAAPVHEPVSVRVQPKKAVEFPAPQSTPV
                     APQKKKRIFLKVAAAVLIVAVLGGGGYWAYNEFFGGVRKKLLVEQKIEVSDKDQTVKY
                     EDEIAVTVPFGLIDKEQTLSIYSVKGMPEEEGIKQLGAYDVSMSETNEFDGYMEVTIK
                     YNPADLPAGADPSKDLFCMYLDEASNEWKGLPYTVNAADNTMTIYTNHFTTFAPWAVS
                     EKVAPGPMMTVQKVQFPGGAFMSSDEVVSTMEEFAAAAPKSDGAVRAGWDKVNEWFGI
                     SAAVGSFAENALEMGALKGINEVATEVGLGFALVQCAIDVADGKTGKATLELSKNLYN
                     YWALKLINTSAINLAFVGVFVIDWSLNKFIQEAISGRVNIYQKAYDLYYKEKREKEKI
                     NSVYWYKKLKKVMKSVKNPATASEEVIKVIHDYVWEFWKDESVVGEYQDRVMKNTGAT
                     GGGYLNEQLKKDISDEHFASIIKTLNETNIFERIVKEIRLDMQGKLYDKLCYIQKELN
                     KVNKIRVIVKIDPECEEYADVDVSGLAVQFNVASDVHKKLWTGTTDKDGEFLFNCTTL
                     GFLDAGSPQEVIVTVAGPAGKDEEFTGELNLEGNGKTTVVEIMIGSPKLEGTWKLDAT
                     VTKMTMDASLQYIDNMADMYGSGDEYYKEREKVEQEMKGKKAQLPDLRLDGIEYMMDV
                     KKEGQYYVIQSKRFDDPEALGVVQYKIRFTGRNSFEGKYVGINHLRGKENRTEMDIKG
                     TRIK"
     gene            59108..59833
                     /locus_tag="PKY63_01175"
     CDS             59108..59833
                     /locus_tag="PKY63_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99252.1"
                     /translation="MIRNKYRIIANRSAVFFGAIALLLFLLPSITGTDGMNGGFALIV
                     FAIVFLITGLITFYFYNRNARVFDSLLQPQKILAHWVVKQNEYAQLQQLNAKEISGNF
                     MLMRWVVIIITFIVCVFLAIAGLEFSFLLLFFAGLSAFIWIVSAIAIHSQKRHLLPTD
                     AQIILTNEGGFINDTMHVWAKLNNRLEDAAIKKIKSGIHYLEINYSVPSRGARVETTV
                     RFPIPDGKLTEAQYILGEIYNPQ"
     gene            59842..60663
                     /locus_tag="PKY63_01180"
     CDS             59842..60663
                     /locus_tag="PKY63_01180"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPE99253.1"
                     /translation="MKNNNIKTLIEAAWDNRELLNNTETRDAIRAVIDLLDKGEIRVA
                     EPKGDKWQVNEWIKKAVILYFPISEMIVSEAGPLEFYDKIPLKKGYKKAGIRVVPHAV
                     ARHGAYIASGVIMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVL
                     EPVQAAPVIVEDGCFIGSRCIVVEGAHIGQEAVLGANTVITGSTKIIDVSTEKTIEYK
                     GSIPPRSVVIPGSYTKKFPGGEFNVNCALIIGQRKESTDRKTSLNDALRNFNVDV"
     gene            60708..63428
                     /locus_tag="PKY63_01185"
     CDS             60708..63428
                     /locus_tag="PKY63_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99254.1"
                     /translation="MTKTFSKIASILVFISISLCSWGQRTTILNNEFSYTSTYQYVST
                     PGAREFYSNFIIQEIARSIPKRAEYTSFTINYIMNQQLIKLDSAHYIFKPELKNFATT
                     GDVLYKGINISKKLIPGEIDYTVKIYRKQSGTMNIGGQQNGILVFQKNVTGQQLNDNL
                     GYFPLPELAFSDTVNDAQYTLAVENQHLYFNEKSKNIFLNQVAYINDYYASDALIGAQ
                     MDKLHAIQPDNIDLISFYDIELKEVEQSIASLDSRQFPQNLGLHENDPMQFMAKMHTL
                     VTETSQMRATLNQRLSVLDQLFCQKGNDLLAEGKLQDAATYYNKAIQINPYYAPAQYQ
                     LARMEFNAANYDTSAYMVNHALLKMSPDPSTQQLLVQLGNNIYTTLLGNGDKYNSEQK
                     FNEAISVFDRAKWLCTTTPGLVCTEQVQKGLAQAKYGIYRSYLQVAEKAIQNGRLDIA
                     GNYINQSMEYQKQNATEIISNAESVQWMGILCKEYVMLGNKLNQQKKYDQAIIEFNKC
                     DSISQVYPSIGEVKGCQTGLCGARNGIYNNYLEDARDRLGKDQLDAAEEKCLTAMAYQ
                     KQYKDDIFSDRDATEVMALIKEKRYHIAIDNGKNYLNGNNHSEAAKFFLEANGYQQNY
                     NFRRNDSLGIYIRTAGKPLVAAIVEEGKVQAWGNKLAEARNSYQKANDDIKLYGLEKD
                     KELEASMNELKNKIFSQECANAEQSYLSFLDKSKKLMRDGDYALAYLTLSQAIDITSK
                     NVLCNIDNSTAYKEQNRIKPAMEYQNLMFKVDEYSAQGNYTECLKQINNCGIFYTTNG
                     IDSFGLKHPTPQEYILRGGDVNFVYFGADHFYGLKDYESAFKMLQELKSRNYPVKFTK
                     TIQTQLGGKMAIRDKINGVTNYKIQILKYTEGEKWYAVFSKAYKKSWKKN"
BASE COUNT        18593 a        14463 c        13035 g        17262 t
ORIGIN      
        1 ccatctgggc agtggattct gtctgctacg gcgagaccat tgatatttat gcaaccggct
       61 ctgaaaccta tacatggaac accggatcga ccaatgattc cattcagata accgttgttt
      121 cggataccgc catcagcgtg gtggccacct atctcacctg cattgaacgc gacaccattt
      181 acatcaaagt caagccattg cccgtcatca cattcaccgg cgactcgtcg gtttgtctca
      241 acgattccat gcatctgggt gttgatggcg gtatcaacta catctgggaa aatggtttta
      301 caaccgatag catttcgttt ttgccagttg ccagcgaaac actgacggtt actgttacaa
      361 acagctacgg ttgtacagct caggacagca ttcagactac ggtgtatcca ttgccggtgc
      421 cactgatcac gggcgacaac acgccctgtc tgcatgatac aaccagtctt tcggccagcg
      481 gcggagtatc gtttgaatgg tctaacgcag ccacaactcc cacaatcgat attgcgtgga
      541 acagcaccgg aacttttgac tacaatgtaa tcgccaccga tttgaacgga tgttcggaca
      601 ctgcattttt cacagctacg gttacaaacc tgccagaatt ctggcttggc aacgatacca
      661 tcatttgcga cctgacaaac attacgcttg atccaggtat tgcaggtgcc agttacaact
      721 ggagcaccgg agctgtgacg caaacgttgg atgtttcgtc ggccaacacc tactggctcg
      781 atctgaacga tgccaacaac tgtcacttcc gtgacaccat cgatattgca gttcaggcgc
      841 tgcccgacat cagtttcagc ggaaccgaca ccacctgttt tgagtcaacg attaccataa
      901 ccgccacagc tccaagcgga gtaaactatt tctggagcaa tggtatgaat acgtcaacca
      961 ttacctttac gcctgctgca agcggtcttg acactatcag cgtaaccgta actgacgcat
     1021 tgacatgttc aaaaaccgat agtcttgaat tcatggttta tccattgcct ttaccggtaa
     1081 ttgcaggcaa ccccactccc tgtctgcatg atacaaccac ccttacagcc agcggcggtg
     1141 aaagttatat ctggtcaagc ggagcaacga caacatcgtt ggacattccg tttgacagca
     1201 tgggaacata tgagtatttt acaattgcaa cagatattca cggatgcagt gatacagcgt
     1261 tctatacagc aagcgttctg ccactgccgg tatttgatct tggcgcagac actacaattt
     1321 gtgaatccac atcaattagt ctgcatccag gtatcacagg agccatatat gactggagca
     1381 caagtgaaac aacccaaagc atcaatgtaa gtctggcgaa tacatatcat cttgaaatta
     1441 ccggaatgaa cggatgcaga tattcagatt ccataaccgt ggcagttcaa ccctatccaa
     1501 ccattacctt tggaggcgac acaacggtct gctacagtca tccgattacc attacagctt
     1561 caggaggcgt aagctatgtt tgggaaaaca gtctgtcagg cgacagcatt gtattcacgc
     1621 ccctttcgga tgatacctta tcagtactcg ttacagacat atatggttgt tccagcactg
     1681 attccatcag ttttgaagta ttacccctgc caacaccaaa catttcaggc gacacagaaa
     1741 tatgccaatt cgacacaacc acactgacag catcgggcgg cattacgtat caatggtcaa
     1801 caggacaaaa cacgtcaaca gccagcattc ttccttcggt tgccggaatc aatcagattt
     1861 atgttattgc gacaggagcc aatatgtgtt atgatactgc attccatcag ataacggttc
     1921 atgcattgcc ggccataaat cttggaccgg acaccaccat ctgtgaagga acatcgctat
     1981 tgctggatgc agcaaaccca ggttcaacat acagctggaa cacaggtgca tcatcacaaa
     2041 cccttacaat cactgcagca ggaacatatt ttgtttcggt tgcagatgcg aacacctgcg
     2101 aaaacaccga ttccattgtc atatcaacac agccctatgc cgatgccagc atcacagata
     2161 taagctatgt atgcctcaat ggctcaccct ttgcattcgt tgcggcacaa ggcggaggaa
     2221 gctggagcgg tgatggcata acaaacgctt caagcggaga attcaatccg gctgtagcag
     2281 gagtaggtta ttcaatcatt gtttacacca tatccggaat gtgcggagat aaggattcca
     2341 cgaccattga ggtagccgac attccattta tcgattatac attaagcgat gaaacctgtc
     2401 cgggatacaa tgacggaacg ctgattttgg aaatttcggg tggaacctcg ccctatttct
     2461 atgatttaag tggaacaaca attacagaca caaccattaa tcttgcaccc gatggatatt
     2521 tgctgacagt aaccgatagc cgcggatgta tgacttcgga cagtgtaatc attcatgcag
     2581 aagacactcc ctgcggcgaa gtagaactct ttattccaaa cattttcagt ccaaacggtg
     2641 atcttgaaaa tgatgtactt tttgtccgca gcaattttat tgaaaccatg gcctggtata
     2701 tctatgaccg ttatggtgaa aaggtttttg aaagcaagga tatcaatctt ggctgggatg
     2761 gcgagtttga aggacaaaag gttcagaatg gagtttattt ctggcacatc aaagccacaa
     2821 tgattgacgg ttctgtcatt gagcgcgaag gcaatgtaac agttgtccga taaaacagtt
     2881 ttttttcata ctgtgctgtt tttttctgat ttcacctgac atcgtgcatg gcaagcatga
     2941 gcaagggaag gttctgtcca tttccatcag gatgagattg ttaaaaatat tacctttgta
     3001 caaatgaatt cagaattaag taaaactgat ggcaggatgc tgcctgtgat ggaagaattc
     3061 ccaagtttgc agggagaagg tgtgaatacc ggaaaagccg catggtttgt acgtttaggt
     3121 ggatgcgatg ttggatgtag tttttgtgat gtaaaagaat cctggaatcc cgacatacat
     3181 ccgttgtcac cagccgatgg catagtcgaa aatgtggtaa aatcaaaaat tggtgctgtt
     3241 gttttaaccg gtggagaacc tatgaaatgg aatcttgatt acctgaccgg cgaactgcat
     3301 aagaaaagca ttaccatttt tctcgaaacc tctggcagtg aaaaacccag cggaacattt
     3361 gaatggatat gcatcagccc aaaaaaagga accatcgttg attcttattg gtttgaacat
     3421 gccaacgaac tcaaggtaat tattcaaaac gaaaatgaca tggtttttgc agaagaactt
     3481 tcaaatcgtg tcaatgccgg ctgtattatt caattacaac ccgaatggag tgtcagaaac
     3541 caaatcatgc cgtaccttgt tcagtatgtc atagaccatc ctaaatggcg cttatcggtg
     3601 cagacacaca aatacataaa aatcccctga tgccatgaaa aacattttaa tttttggcct
     3661 ttttttactg atgacagtat gtatgcaggc acaggacaga cctttgacct cgaaaaacaa
     3721 aaaagcaatc aaagagtatt atgcaggagt tgatttcctg gacagatttg tttacgatga
     3781 tgccataagt tcgtttgaaa aagccattct gtatgatccg gaatttatag aaccctatat
     3841 cctgctgggg actctgtacg aagaaaaaaa agaatacacc aaagccattg aatactacga
     3901 aaaaagtctg acgctggatc ctgaattttt cccatcaaac ttttacaatg tcggattgct
     3961 tgaaataaaa accggacaat atgaaaaagc gcttaataat ttccagacat ttctgaaata
     4021 caatcccaaa ccagggccaa aaaaacaggc agaacagagc atccgaattt gtcaatttgc
     4081 cattgaagag attaagaatc caaaaccgtt tgaaccaaaa ccgcttggaa caggcgttaa
     4141 tacagagaat tcggaataca tgccagcact tacggccgat gaatcgacca tcattttcac
     4201 acgactgatt aaaagcacat cggcagagag ctacagtgaa tatcaggaag actttttcac
     4261 cagcaagctg attgatggtc aatggagtac cgccattccg ctgagcaaaa aaataaacag
     4321 tcctttaaac gaaggcgccc attgcatttc acctgacggg aaaatactgt attttactgc
     4381 ttgtgggcgc aataacaata aaagttgtga tatctacatg agcaagctgc gccatggaga
     4441 atgggattat ccgctcatag ttccgaaaat caacacagaa gcatgggaaa gtcagccaag
     4501 catcagtgcc gacggcaaaa ccatgtattt cgtttccgga agatataata agaattatga
     4561 tttgtttgta agcacctata atgattcaac cggctactgg ggagaaccgg aaagacttcc
     4621 ggacaacatc aattcgagcg gtgttgaaca aactccattc attcatcatg atggtattac
     4681 actgtatttc acatccaacg gtcatatcgg catgggcggt caggatattt tttacacaaa
     4741 aaaaatagat gacacaacgt ggagcgaccc ggtcaacatc ggttatccca taaacacaca
     4801 tgccgatgaa gcatgcctgc atatttccgc atctggggaa acagcatatt ttgcaacagg
     4861 caatctggac gtggaaaaac aggaagtatg gaatatggac atttacagct ttgatttata
     4921 ccgcgaagca cgccccattc cggttacatt tctcaaaggg attgtatcgg acatcaacac
     4981 caaacaaaca ctgcaggcct cctttgaact tacggatctg aagagcggaa agattgtggc
     5041 caaatccact tcggatgaaa acggtgcctt tctgctgtgc attcctaccg gcatggatta
     5101 tatgttgaat gtttctgctg aaaaatatct tttttattct gattttttca gtctttccgg
     5161 aacacacacc aaaactgatc ctctcattaa aaatataagt ctgcagccca ttggaagtga
     5221 tgccgtggtt gtgctcaaca atatattttt cgataccgat caatacgatt taaaacagga
     5281 atcattgatt gaactggata aagtgaaaaa aatgatgctg tccaatccga aaatgaaaat
     5341 tgaaatccgc ggccacaccg acaatacagg aaacaagcaa cacaacctga ccttgtcgga
     5401 aaacagagca aaagccgtgg tcatgtatct ttgttcacag ggtattgcca aagagcgttt
     5461 ggtttataaa ggttatggcg acagcatgcc acgcgccacc aacgaaaccg ctgaaggccg
     5521 cgctctgaac cgcagaacag aattcaaagt aataagcaac taaaaaaatg attaacaaac
     5581 aaaaaattga aaagtggaat caggaaacag ccaaactcaa tcctgagcaa attattgatt
     5641 atgcattccg caacatcgat ggccctgtta ccatggcaac cagtctgagt attgaagatc
     5701 aatatttgtt atacctgatt ctgagaaatg gccatgaatg cagctttttt acgctcgaca
     5761 caggtcgcct gcctcaggaa acatacgata ctattgctgc aacagaacaa catttcggta
     5821 tcagaattca ttcgtatttt ccagacagac atgaagtgga agcttttgta aaccaaaacg
     5881 gagtcaattg tttttatgaa agctatgaac tacgcaaaac atgctgcagg attcgtaaaa
     5941 caattccaat ggacagggca ctggcaggaa aacaggcgtg gttcaccgga ctacgacgcg
     6001 aacaatcggt aacacgcatc aatattccca tctttgattt gaacgaaaat accggaatca
     6061 taaaaatcag tccattggcc gatatgtttg aaaaagaagt ctggaccgaa attgaggaaa
     6121 acaatattcc tttcaatcca ttgcagaaaa aaggataccg cagtctgggc tgtcttccct
     6181 gcacacgccc gatcagcgac gatgatgatt ttcgtgcagg ccgctggtgg tgggaaaatc
     6241 cagagaacaa ggagtgtgga atccataaga aataagattc aagatcaagg tcggggtcag
     6301 gcagtaatca gctctgagac ggggctgaag cgttacaaac gttactgtcg ttacagtcgt
     6361 tacgcattac aatcgttacg ggtggaattt ataacactga aagcgggatt cgatacaaat
     6421 agctctcagc cgtcagtttt cagtaatcag ctctgagaag gagctggttt tttttgaaat
     6481 tcaaatcagc gcataaaatt gtatttttgc ggcatcttgt attttcacat ctccgcattt
     6541 ccacatttcc acatactttc gtatttctga tctcgtattt cgtatttcgt atttcaaaga
     6601 gtctgttcat gaatccaaat ctcattctac tcgtttttgt tatttacacg ctgctgttgt
     6661 ttttcatcac ctggctgacc agccgcaagg caaacaatga aacatacttt atcggaaacc
     6721 gtcgctcaat ctggtatgta gtggcctatg gaatgatcgg cgcttcgttg tcgggggtta
     6781 ctttcatgtc ggtgccgggc atggtggaac aacacgggtt tgcgtatttc atggtagtca
     6841 tcggatattt tatcggatat caggtagtgg cttttgtgtt gctgccgctg tattacaaac
     6901 tgaatctcac atccatatac acttacctcg atcaacgttt cggcaactcc tcctacaaaa
     6961 cgggctcgct gctgttcatt ctttcacgct cgctgggcgc ctcgatgcgc atgtttcttg
     7021 tagtgctgat cttgcagaat tttctgttcg acagtcttgg aatacccttc gcattgactg
     7081 ccatcatttt catttcgctt gtattgctct atacttttca aggtggcatc aaaaccattg
     7141 tctggactga tacactgcag accacattta tgctcggtgc tctgatattc accttatttg
     7201 ccatagcatc acagctgaat ataagcatcg gtgatctttt ttccaccatt gccgacagca
     7261 aatattccaa cgtcattgtg aatgatttca attcgaagga tcactggctg aaacaactgt
     7321 taagcggcat tttcatcacc atcaccatga ccggcctcga tcaggaaatg atgcagaaaa
     7381 atctgagctg ccgaaatctg cgcgatgcgc agaaaaacat ggtaactttt agttttattg
     7441 ttgttggtgt cacattattg tttctggttc tgggagccgc tttgtacatt tacactgaca
     7501 cgttgaatat tgtacgtccc gcgctggcag acgatttatt tgcaaccgta agtttcaatt
     7561 atctgccggc catagccgga tttgtatttc tgattggctt gatttcggcg gcctatccca
     7621 gtgccgatgg cgccatcacg tcgctgacta cctcattttc gctcgatatt ctgcgcatca
     7681 accgcaaaga atacagcgag aaaaaacaaa agcgcatccg gctactggtt cacttctcgt
     7741 ttgcattgct gctacttggg ctcatcattt tattcagcac catcaacgat cgcagcatca
     7801 tcgataaatt attcaccatt gccggataca cctacggccc gctgctgggg ctgtttgcgt
     7861 ttggtttgta caccagtcgg ccaatccgcg acaagctggt tttcattccg gtcattgtgt
     7921 cgccggtact ttgttatctg ctggccgcca acagcaaaga atggtttggc ggctatcagg
     7981 ttggttttga actgctgatt atcaacggag cattgacgtt tgttgggctg tgggggctgt
     8041 cgaggggttc ggtgcgaagt taggaaactt cgcacagcgt agtgttggaa tcttcgctcg
     8101 gacgaagttt cgctaaaatc aaggctcaga aatacttttg gagtttttta ccgcattttc
     8161 aaagactttt aagtgggttt ttatcgcgat ttgcaaattt ttaagtgggt atttatttgt
     8221 tttttttgat tttttcccag ataaatgttt atctttgtac ttaatatgga aagaaaagta
     8281 tataaaaagt tgctcgactg gaagaaaacc agaaaacgca aaccgctttt gttgcaaggt
     8341 gcaaggcagg ttggaaaaac gtatttgtta aaagaattcg gcaaaaaaga gtacagtaaa
     8401 gtattttatc tgaacttcga atccgacaaa cgcctggaca gtttgttcag aacaagtatt
     8461 gagccggcgg cgatcatttc gaaacttcga atctttttaa atgaagactt tgatgttgat
     8521 acaaccttat tgatttttga tgaaattcag tcatcggagc gggcgttatc gtctcttaaa
     8581 tattttaatg aagacgaaaa cgaataccac attgctgcgg cggggagttt gctggggata
     8641 gccatgaaca gaaaagaata ttcgtttccg gttggccaaa cgcaaatgat gcatgtattt
     8701 cccatggact ttgaggagtt tttaatggct gttggtaagt ccaaacttgt tgaggaaatc
     8761 agaaagtgtt atgaaaacga tgcattttgc gcatttcatg aaatggcatt atcattattt
     8821 cacgattatc tgataaccgg cggaatgccg gaagttgttg ctgaattcgt aaagaacaat
     8881 gatcatctga aagctgcccg tatacagcag caaattcttg attcatacat tgccgatatg
     8941 gcaaaatatg ctactcccga agaagtgagc aaaataatcg ccagctacaa ctcacttccg
     9001 gcacaacttg caaaagaaaa ccggaaattt caatataagg ttgttcagaa aggcggttat
     9061 gcgggaatgt ttggtgcagc cattgattgg ctttcttcag ccggacttgt gtacaaatgt
     9121 aaccgggtaa cggttcccaa tgaaccatta tcagtctatt ccgatatgac tgggttcaaa
     9181 ctatacatga acgatgcagg attattggtg aagaaatccg gaatgccgct cagagagata
     9241 ctgattgaaa ataagcacac gggctctttt ataggatcag ttgtagaaaa ttttgtggca
     9301 gcggcttttc aatctcttgg tataccgctt ttttactggg attcaggcaa caccgccgag
     9361 gtcgattttc tgattcaggg gcaatctggt gtaattccgg ttgaagtgaa atctgtcacc
     9421 aacacccgat cgcgaagcat tgatgttttt agaaaaaaat acaactcatc aaaaatcata
     9481 agattgtctg aaaagaattt tggatttgac aatcagataa aatcggttcc attatatgct
     9541 gctttttgca tggatagcga attgtagttc tgtagcgctg ctcaaaatct gagtcttcga
     9601 acaagttcaa tttattttgc tatttttgat tttcggaaac aacgcccatg gaaataaaac
     9661 gactcttcga tttactcgat cgctacaagg agaaattcaa caagcctgtg ctttttggtg
     9721 gaaaagtcga tggcatctgg cagcgctaca gcacgcagca acatatcgta aactcaacga
     9781 acatcagcta cggcttgatg cagcttggca ttcagcccgg tgataaaatc atttccatca
     9841 ccaccaaccg gcctgaatgg aattttctcg acatgggtat tatgatgtgt ggagccgttc
     9901 atgttccaat atatccaaac atcagctcag gcgaatacaa acatattatc aatcattccg
     9961 aagcgaaaat ggtttttgtt gccggtgaag acatgtactc ccgcatcaag gatattttgg
    10021 cggaatgtaa aaacattgaa tcagtttaca cattccggaa cctgcatggg ataaggcatt
    10081 taaacgaact catggaaatg ggcaggcaaa acccgcaatc agaaaagctg caatccatca
    10141 aagactccat taaagaagat gatgttgcca ccatcattta tacgagcggc accaccggaa
    10201 ttcccaaagg ggttatgctg acacacaaaa atctattgag caatgtggag gccatttaca
    10261 ccatcccggc acaaaatccg gcactggtaa cgctgagctt tttgccactt tgccatgtgc
    10321 tggaacgcat catgaattat atgtatcaat acaatggata tacagtgtat tatgtagata
    10381 atatagcact gatatccgat gccatgcgcg aaaccaatcc ttctattgta tgctgtgtgc
    10441 ctcgtgtact cgaaagcatt catgacaaaa ttatggcgac agggcgcaaa ctcacaggat
    10501 acgaaagatt ctttttttat tgggccgtaa atatcggtta tcgctatcgc atcgaaggca
    10561 ataatatttt ctaccgtatg cagctctggc tgctacgtaa gctggttttt aaaaaatgga
    10621 aacaagccct tggtggcaaa cttgatatta ttgttagtgg tggagccgcg cttcaggagc
    10681 ggcttgcacg cattttttgg gcagcaggat tccgtgtgct tgaagggtat ggactcaccg
    10741 aaaccagtcc tgtaatatct gtcagcaact ttgaaaaaaa cggaattgaa ttcggaacag
    10801 tgggtccgcc gttaaaaaat gtagaggttc gcattgctga tgacggcgaa attcaatgca
    10861 aagggcctaa tgtgatgaag ggctatttta aggatgccgg acaaacaaaa gaagtgctga
    10921 gcgaagacgg atggttcaaa accggggata tcgggtttat tacccaacat ggtcatgtaa
    10981 aaatcactga ccgcaaaaaa gagattttca aaacagcagc aggtaaatat atttcaccac
    11041 aactgataga aaataaactg aaggaatcac cgtttatcga caatgccatg gtggttggag
    11101 aaaatcagaa atatccggca gccatcatat cgctcaactt ttttcatgta cgctcctggt
    11161 gtgaaattaa aaaaatccca ttcacaacca acgaagacat tctgcaaaac ccagttatca
    11221 aaaaacggat tgcagttgaa attgaaaagg tgaatttaaa actcggagag catgaaagaa
    11281 tagtaaaatt cgagttcacg ggcaatacat ggtcggtcga taccggtgag ctgactccaa
    11341 ctttaaagct tcggcgtaaa ataatttcag aaaaatacag ttctttatta agcaaattat
    11401 tttaaaacca accttatgaa aaaattcatt ctcttgctgt caatcatttc agttgtaatg
    11461 ctgaattcat gtaaaaagga ccaaaatgct gaaatcaagg tcctgactga acatatcgaa
    11521 tatgatgttc tgatcaataa tgacggaaaa atggatcccg tcatgaatca tgtaaacgaa
    11581 gatgttcgtg ttgaatttgt tcatttcctt tttgaacagc tcaaagcggg aaaagcattt
    11641 aacgacagcg gggcttcaat tgattcaaaa gcagtgctga tgctgatccg cgaaatgttt
    11701 cccaatgccg acacaagtgt aatcgatccc gaagtgtact acaaaatcaa tactgccaaa
    11761 atcaacaaac tcagattccg tgaaaaatgg atttacaatt cagaaaactt caaaattgaa
    11821 aaaacagtgc tggctgtagc accactggtt gaactggccg atacactcgg atatgtattc
    11881 aaagctgaac ctttattttg ggttcaatgc gatacagcta aagaactgaa agaagttacc
    11941 gtattgtcaa ccaatatcat cagcgatgct tttgttcaca atcaacttga aatgattctt
    12001 tacatggact cgactccggc cgattattat tgtaatctga atgattctgc caaaacagca
    12061 tactttgatg aactggtgaa atcagtgatc gataaaaaaa taaacgctta caatttcttc
    12121 ttcaacccat tggaagaagc cgatatgaaa gttctgaaag gatatgccga cacattgtcc
    12181 gactttgatg aaaacaacaa tccggttaaa acggtcatcg aacataaaat atctgccaaa
    12241 gaattcggtc gcatcaaatt tgccgaacga tgggaatact ccgcttatcc ttttgttttc
    12301 cgcaaaacgg tgatggctct caatccgtcc gtgattgtca tcgatccgca atatgatgtt
    12361 gttaagggct tcaaaccctt gttctggaca gtatatgacg aaaaatacct acaggaaatg
    12421 aaagggaaaa ttttgcagta aaaagtcatc agttatcagt gtgttttcac tcataactca
    12481 tagcttatca ctcataaccc aacactccct aatattccgc tttcacgaac tttgcacgca
    12541 gcatagttcc gttttcaatg accgttataa aatacagtcc ctgtgcgaga tttgagatat
    12601 tcaatgcgcc gccttcgcaa ataccctgct gcacaatatt gcccgaaata tcagaaataa
    12661 aataatcgcc ctgtgctaca ttgctcagca aaagctcatt ggtggccgga ttcggatgca
    12721 gcttcagtat attcagttcc gtttcatcga tgccaaccgc ctgaataata ttcagtgtga
    12781 agtcgtcgaa ataaaatccg tcgcgctgct catagctgtc actcaccagg cggaatctga
    12841 gtttgcggtc tgcgtacaga taatctgtca ggtctatgta ttccttcacc caggtgctct
    12901 gcgtgccgtc gtaaagcggt tcatcctgca gctgatcggc gcttccgcgg gtggtatatt
    12961 gtccgcattg cggaatccag gtggcaccgt tgtcggtcga aatttcgcaa acgcaatagt
    13021 catatccttc ttcaatatcc cattttgcat aaaactcaat ccacgccttt tcggccagca
    13081 cactgccaag cggcaaggtc aacgtcatgg atgaatttgt attgttgctg tacattccgc
    13141 cgggactatc ggtaaaagaa tagggcgatg aaacaaaatc ggaaccggtt gtcgaccagc
    13201 caccagccca gtttgtagtg tttgtggcat tgtcgctgaa cagaacagaa gacatcccgg
    13261 aatatttcac caatgtgtct cttgttgtat acactccgtt gtttacgctg agttcaagcc
    13321 ggatttcatc gccctgcgca taaggaattg cgagtgaaat atcaaaagtg tccagtcgtg
    13381 tttcaagcaa agccaggtcc gtaaacgtaa taggggatgt cattaccatt acattcggag
    13441 tcagtgccgt gagcgaaacc gtataaacag ccggtgtatc cattcccagc cgttgaatac
    13501 tgaatggaat aatccaggga gagcaaagct ccgataaatc cttcacttcg gcatatttgc
    13561 aggcaaaacg gaccatgtac agattttcgg acacgtacaa tttgcagata tcgtcaatca
    13621 acgcttcggc aggccagaag ccgtctgcag gatcccctac ctcggggccc catgcaatga
    13681 ttttgtcttt ctcggtctgc tcgccataat accagtcgaa tgaaccacca ttggcattgt
    13741 agccgataca ctgcatcggc gttccatagg caaacgaatt ttccgaagcc agatatgacg
    13801 aatacgtgcg atacaaaata tcatcaggcg ttggtaaatc ggcatacccc cacgaataca
    13861 gaatcacatt ggaataggtg tgataatcct gtatcagttg aatgtcgtga gccagcgtaa
    13921 aattgcgcac agcctgcgtt tcggcttcgg aaaaagcaga atctccacga taggtttcat
    13981 ctgttccgaa tggtgatgaa ccaatatcgt cgaatcccca ctggtagcca taattccggt
    14041 tgagatcgac gccataactt ccatcgccgt tgtttctccg gttcttgcgc cacatgccgc
    14101 ctccggaagg attggtggtg cggttgtact cgtagccgtc aggattcaca cagggaataa
    14161 aataaatttc ggtattctgc agcaaatact gaatctcagg atttgtcgca taattttcca
    14221 tcagatacca catgaaaaat atttcctgct gcatggccat gggctcgcgc gcgtgattca
    14281 gcgccagaaa catcacttcg ggttcggttt catccacatc gggattgtca gagattttta
    14341 cataatacaa ctgacgtccg ccaatggtgt gaatggtatc gacaatctgc cgcgccgaaa
    14401 tgaggtttgg aaacaccgct cgcatttcat cgagctcggc atataattca gcaagtgtca
    14461 gaaacccgcc catggagcca tggttgaaat gtgccggcac ggcataatcc ggaatttcga
    14521 aacagccggt attgcgcata tttcgtggat tcatcgattg gttctgcttt atataatgtt
    14581 gctgcacatc agaaataatg acttcaactg tcaggccggc atcgcgcatt ttctgaatct
    14641 gtgtttgcga aaagtcgcag atcagatatt cgcctttctt gatttcacct tccaccggaa
    14701 tgcccagcga tccgatggta ttcaggaaat cagcgctgcc actgactttt gcgcgttgat
    14761 aaatttcctg actgttggca gtccaggcca taataacaaa taagaacaga ataataccag
    14821 ttttcataca agaggttttg gaataagaca atacattgaa aattatgttg caatgtacga
    14881 aatgaaaatg gattggagtt ttaaccttcg aagggtttga gacgcttcaa tgattataat
    14941 cataatgaat tcatcaccac caatttccct tgcttcaaca atttttcgct tatgcgttta
    15001 atgatgctgc cgcggctcag taagccctgt tgacggaatt caatacggtc ttcggaaaca
    15061 ttgtcggcca tgcccatcca gatattgacc ttgtcggcca ttttcagaaa ataggtcatt
    15121 tcgtatgccg gcgatcctgt cggaagcgcg gccagatctt cgtcgagcag attgcaaacc
    15181 tgattcagtg tcaccattcc ttcggtgatc agatcggttc ccttcatttt aaatgaaggc
    15241 ggagtcaggg catcgccgtc atcaaccaca tcgatggaat gacctgtaac gcgtgctgcc
    15301 attttcgatg tggacccgcc actgataata tgaatgccag gttcattata aaatgcgtgc
    15361 acgaaatttt catcatcagt agcatgcaac ggaggaccac tgagcagatt caccaccacc
    15421 ccacgacgat tgacagccac caccacggaa caatcatcgc ctttgttctg cggccagtag
    15481 cttcgtgcct gattgtgtac cagctgagcc agatcgtttc cgctgatacg ttttccggtc
    15541 aggtgagatt gcaaaaaact gcacacaccc tccgattccc agccattggt gaatagtttc
    15601 cctataccgg cctgtgttat cccatcggac atcagcatca gaccttcgcc tttttcaatc
    15661 atgcagcttg attcatgcac cactgccttc tcgcgcgtat agacacgatc gtgcagtacc
    15721 tgcgcatagg cgcggctgat aatcaatggc ggcggcattt catatcccag aatggttgtt
    15781 tgtccgttat tcagaatctg cacaatcgta aaaaccgaaa atggatcaac ggttccccag
    15841 gcgctgtcca tggtggccga tacgcggtta aaagtttcac gaatggaaag gcccattttt
    15901 atcaaagtga gtatgcgcga cacacacaga ttggctgcaa tattggcttt gatgcccgaa
    15961 cccagtccat cagccaaaac caacgtggta gcattttcat tgcggtaaac gccccatgcg
    16021 tctccgcaag gctggccggc cttcttgggc gattgggcag caaatatatc ggtgtgaatg
    16081 tacatgggtt tgacaattaa tttgaatgaa acatcactta ttgattgaat caatcaggtt
    16141 tttcatcaat acttcacctc gtgccgtatt ctcaccgaga aagcgcgcga gttcctgcgc
    16201 catatcaatc tgatgatcga tgagttcctg agcgcgatct accgtttcgc tccgcagtgc
    16261 attcagctta tctttgctcg atttgctgtc agtgacatcg acaaacaaac ccaccatctg
    16321 ttcttcttcc ggaagccggt aagccacggt atgacacatc agattataat tcggatagtg
    16381 attgtattcc cgcgtggcat cttcctttcc tgcaatcagg ttttcgaatt gtttgggatc
    16441 aatcaggtaa ctgattttac ggcctaccag cgaatcggaa catgaaaaca tgtgtttgaa
    16501 cgcctgattc atgcgaatga tttcaagatt ctcgttcaac aaaacaaatc cattcggatc
    16561 acgctcaata atggtatcgg ccttctgctc ggccagacgg cgcatatatg gcatacacat
    16621 ttcgggctct gccattcctt caagcacagc aattgcgcgg tcgcggcacg atttgtaacc
    16681 gcaggcggta caattcaatt catcggccgg aatcagctta cctgttttac tcagtacttc
    16741 acgaatctgc tcttctgaga aaacaggatg atcattgccc gacgccagcg agaaaacaac
    16801 atccgtatcc ttgaacagtg atgatttttc agcttcttcg cccggataag ccgatgcata
    16861 ttccagcacc atgcggcggg cttcaaaact gttgcagttg tgctgcatgg cgggaccatt
    16921 cacacaaccg tttttacaaa acaaaggttc aatcacacaa ttggttgggt tgcggtgtaa
    16981 atcatccagc aattccctga tttcatcggc accactcaat gccattacca cacgtccgaa
    17041 catggaatct gcaatgtttc cggttttcag caaaccactt tccagcggaa acacgcgtgc
    17101 aagtccgttg accggatcat tgaaagtact ctcttcgcac gcatccagac tgatgttgcg
    17161 gagtcggaac aattcatcca gttcttcaaa agtaatcacg gcatcgatat cattctctgt
    17221 ggatttccac ttagcttcat ctttttttgc gatgcaggga ccggcaaaaa caaatttaat
    17281 tcccggatat gtttttttta tcatgcgggc gtgagccacc atgggcgagg caacaggcgc
    17341 cagatacggc atcagatcag gcattttgct tgtaatataa tttactgccg ccggacacgc
    17401 ggtacagatg acaggttgat cgggattatg ttcaatgaat tcagctgtag cctgcgctgt
    17461 gtgccatgct ccggtggctg tttcaccaac tacggaaaaa ccgagcatgc gcaaagccga
    17521 tggcaaacgc ttgcgccgcc actcgggata gtacgctgca aacgaaggtg ccatgctgat
    17581 tgccaaacgt tcgccatctt ccatcatttg caatacacga ccatattcgg tgcggtatgt
    17641 cttggctttt tgcggacaca cggtaatgca agtaccgcac gaaatgcagc gttcagccac
    17701 cacatacgcc tgcccgtctt tcatgcggat ggcattcacc ggacaaactc gcacacagcg
    17761 gtaacaatcg cggcacaaag ccctcgaggt gtacaccacc gaaatattat ccgtttgcat
    17821 cgggttgtta ttcatagcca tttcttttat gttacaaaag tacattttag atacagaagt
    17881 cccggcagta acaaaaagct actgtcgggt acatctgttt gagtcctgat tcaaacatac
    17941 aggtcattca ttttgcaatt gcagacccat tctctttatt tcctgatcga gcagctgctt
    18001 tgctttttcg aaagtcgcat gttcgagtat cttgtcgttc acccgcatca ctggcccgtt
    18061 ctgacaacgt tcgaaacaaa aagtcgcttt tacatccacc atttcttcgt ggccttcgaa
    18121 gcccacaatg tcttcgagtt tattctcact gacataatcg gccagctgat gaagcagttt
    18181 gtgagcgcct ttcatgtagc aattggttcc gacacaaaca tacacttcga ttttcggatg
    18241 ctcggtgtcg cgaatattaa tgacctgatc gttgatgcgc ttgcggctct tgtagtgcgt
    18301 atgcagcaga tggtgcgctt tatcgctgcc cggctcgccg aggaacgatt tatacaattc
    18361 cttgatgtaa ggattgtcct gcgatttatg caattcgagc tgtttatcgg cattgtacaa
    18421 cccctgtgtt cttttctgac gaaaatcggg atcaaagctg accggctgtc caccacctgc
    18481 aatgcagcct ccgggacatg ccatcacttc aatcagatcg tagccgctat cgcctgcaac
    18541 aactttatcg cagatctggc gcgcctttcc aagagtatgt gctataccaa gctttagttt
    18601 tattttacca agcgtcattt ccacagtacg gatgccatct tcgccgcgaa cctgacgaat
    18661 gtcagtatcg atcagggttt caccggtcag tttttcataa gcaaaacgaa gaaccgcttc
    18721 gctcacacca ccggtattgc caaagataac accggctccg gtcttgaatc caagcggtaa
    18781 atcaaacgat tcgggcttga tggttctgaa attgattccg gtttcatgaa tcatgcggcc
    18841 cagttcgtcg gtcgataaaa catagtccac atcctgcatt ccatcatgaa taaattcttc
    18901 gcgacgcgct tcgaattttt tcgccacaca aggcatgata gaaaccacta ccagattttc
    18961 aggctttacg ccaagaatgc cgggcaggat ttctttagct acgctgccaa acatctgctg
    19021 cggcgatttg catgaagaca gattaggtaa taattgcgga tagtattgtt cggcgaactt
    19081 cacccagccc gggcagcacg atgtgaattg cgggagtttt tcacccttga gttttcgtgc
    19141 aatgaattcc gttgcttctt ccaatacagt cagatcagct gcaaacgaag tgtcatacac
    19201 ctgattgaag ccaatgtgtt tgagcgctgc gacaatctgt ccggtcgaaa tggcgccggc
    19261 ttccatgccg aaaccttcgc ccaaagccac tcgtacagca ggagcaattt gcgcaaccac
    19321 tacttttttc tcatcatgaa taacgtccca cactgcttca acttcggatt tcggaaccag
    19381 tgctcccacc gggcatgccg atacacactg accgcagaaa acacattcaa catcgttgag
    19441 acttttgtca aaggcagggc cgacaatcgt gcgcgcaccc cggttaatga aatcaatagc
    19501 acctactgac tgcatttcct tacaggcacg cacacaatcg ccgcacaaca cacatttgtt
    19561 agggtcgcgg ataatactgt gagattcccg atcgaccggc aaatccattt cggttttttt
    19621 gaaacggatt tcgtttacat tcagtcgccg cgccagatcc tgcaatttgc agttgttgga
    19681 acgcgaacaa ctcgggcaat cgattttatg attggcaagc aataattcaa gattgacttt
    19741 acggatttta cggacctcgg ctgtattggt ctgcaccttc attccaggtt taggtttgat
    19801 ggagcaggta gcctgaatgc ccataccgtc gatatcggca atgcaaagac gacaggcacc
    19861 atacacactc agatgcgagt gatagcaaaa ggtcggcatt tctattcccg ctttgcggat
    19921 gacctcgagc aggtttttct cgttttcaac aggaacatcg gttccgtcga ttgttatata
    19981 tcctttggtt tccattgtag ttttgtttta aatcgttaaa aacccatcac cgcattaaac
    20041 ttacacgcct tgtgacattc accgcacttg atgcacagtg caggatcgat gctgtgcgct
    20101 tttttctttt cgcctgaaat ggcattcacc ggacatttgc gggcacaggc tgtacaacca
    20161 acacacttgt ccggatcaat tgtaatgtcc atgagtgctt tgcaattatt ggtcggacaa
    20221 tgcttacgtt caatatgttc gacatattca tcgcggaatt ttgcaatagt cgataatacc
    20281 ggactgggag ctgttttgcc gagaccacat agcgaggctt ttttcaccac atgaccaact
    20341 tccaaaagca gatccatggt tttcatggtt gcatttcctt caatgatatc atcgagcaac
    20401 gcaagcatct gtttggttcc ttcacggcat gggagacatt ttccacaact ttcgttctgc
    20461 gtgaaattca tgaaaaagcg tgcaatctga accatgcagg attcctgatt catgaccacc
    20521 agaccaccgg atccaaccat ggcgcctttg gcaatcagat tgtcgtaatc gagcggcagg
    20581 tctatgtctt ctgcagtcag acagccaccc gaggggccac caatctgcac ggctttaaat
    20641 ccttttgaag aaatttttcc gtcattatcc attacaccac cgccaatgtt gtacacaatt
    20701 tcgcgcaagg ttgtaccaaa cggaacttcg ataagcccgg tgttggccac atgtccggtg
    20761 agtgcaaatg tttttgttcc tttcgaagta gctgttccgt attgattgaa ccatgcagca
    20821 ccattgcgga tgataagcgg cacagcagcc agtgtttcaa cattattgat gacagttggt
    20881 ttttcgaaca atccgcattc ggccgggaat ggtggtttgg gcataggcat acctcgtttc
    20941 ccttcaatcg aagccatgag tgcggtttcc tcaccacaaa caaaagcacc cgcaccttcc
    21001 atcacatgaa tgtacatgct gcgtcccgag ccgaaaacat tatcgcccag aattccggct
    21061 tcgagtgcat cgtcgatggc tttgcgcata cgtttcacgg caagcggata ttcgagacgc
    21121 acataaacat agccttcatc agcattgatg gcacgggcag caatcatcat tccttcgata
    21181 acgcggtggg gattgccttc cataagactg cggtccataa aagcaccggg gtcaccttcg
    21241 tcgccattgc agataacata ttttttatcg gattctacaa cccgcgtcag atgccattta
    21301 cggccggtcg ggaaaccgcc gccgccacga ccgcgaagtc ctgattcaag aatgttgtcg
    21361 cagacctgtt cgtcagtcat tgaattatag gccttttcag cagcgaaata tccgctgtgt
    21421 gaaatatatt cgcgaatatt ttctggatca acatgtccgc attcgcctaa tacaagccgg
    21481 ttctgtagtt tataaaaagg aatatcgagc tgaccacggc tcttttcgcc attggccgga
    21541 ttgacatata aaagacgatc gataacttcg ttggccagaa cagtcttctg gacaatttca
    21601 agtgcgtctt caggtttcac ttttacatac atggtttcat cgggcagaat gtttaccagc
    21661 ggtccctgcg cgcaaaaacc ctgacagcca ctgtgactca gatgtacctt gtctttacag
    21721 tcatggcggt cgagttcaag ttcaacaaac agcgaagcac caagtgtttc gacagcttct
    21781 ttcaaagcat cgtacacggc cagtgagcca ttggcaatac agccggtacc ggcacataca
    21841 atgatgcgtt tggacaccac accggaagcc gatttatact cgtctctgat tttttccagc
    21901 gttgctttat tcatcgtttt catgattgtt tcagattttc ggacattatt ctttcaccac
    21961 agattcttct tccataatcg ctttcaggat ttcgcggcaa cgatcgggcg tgacttcgcc
    22021 atagaccttt tcgttgacca tcatcacggg agccagacca caggcgccaa gacaactgac
    22081 agcttcgacc gtaaacatca tgtcgtcggt ggtgtttttc tgaggagtca ctttcaatgt
    22141 atcatacaaa gtatcgagca gtttcgatga gcctttcaca tgacaggccg taccgtcaca
    22201 tgctttgaca acatattttc ctttggcttc gagcgtaaag tgcgcataaa aagtagccac
    22261 gccgtacact tcggcaatcg gcattccaag cgcggttgca atataggtta aaacctgggg
    22321 aggtaaaaag cgatatgcat cctgcacctg ttgcaggatg ggaatcagcc gggctttgtt
    22381 gttgccgttg gcttcgagaa tttcatgcac ctgctgaaat tgtctcagac ttttatttgg
    22441 ttcggttttc atggttgtat tatttttttg aacctttttt cgtttggggt tctgattgtt
    22501 cgagtttgtt tacaatgacg gccagattgg cgtagaaatg cgtgtcctgc agaataatgt
    22561 tttcagggat gcggagcgct accggagcga aattccagat ggctttgatc ccccacacca
    22621 ccatttgctc ggccaccgat tgtgccgctt cggccggtgt ggtaataata ccaatgggaa
    22681 tatgcagccg ctgcaccagg tccgaaaact tattcatgtt gtacacttta ataccggaaa
    22741 tttcggttcc gattttagct ggatcggtat cgaaagccgc aacaatattg actcccgctt
    22801 cacggaaacc cggatactga atgagtgcac tgcccagaaa tcctgcaccg accagaaaag
    22861 cttcgtgctg gcggtgatat cccagaaagc gctcaagtgc gaaaagcaat tcttctacaa
    22921 catagcccac gcgcggcttt ccggtgatgc ctgtataagc caggtctttc accacctgtg
    22981 tcgggtcaat gcgcaaaaca cggccaatga ccggcgctga aatattgggc tcaccattgc
    23041 ggctgacttc tctgattaaa ttcaggtact gcggcatacg tctaaccgta ggttccggta
    23101 tgccttgcgg aacgtttttc tgattgatgc tttttgcttc catagtgttc cttttttgat
    23161 gacacaaagg tatattcatt tttcttatta aagatatttt tctttattta tttttcgaga
    23221 attctatcaa gacatctgaa atgtgtaaca aaaaggcctt tttgtgtata tttttaatat
    23281 tttcgtacac aaaaaacgaa aagaaaaaaa tatttattta ttgacatttt tctttattaa
    23341 aaacgactgt acattatata atggcgaaaa gaaaatacat caactaaata ttgtactttt
    23401 gaaatcatat ggacatcagg gactttatac agaacgatcg ctttgcaaaa ctgctgggca
    23461 ttgagcttgt tgaagcagcg cccggacatg cgattacaca aatgcaaatc accgacgaac
    23521 atctgaatgc cgtgaacata gcgcagggcg gcgtgttgtt taccctggcc gatctggctc
    23581 tggctgcggc ttcgaatgca catggcccgg tggcggtttc cattcagtcc gaaataaaat
    23641 atttcaaacc tgccggaaaa gggctgctga cggccgaagc cagcgaaata tcatgcagca
    23701 gcaaattggc aacttattcg atagacatcc gtaataaaga cggagagctg gttgcagctt
    23761 ttcaggggat ggtgtatcgg aacgaataaa tgagtttacc tgattatcgt ttttttcacg
    23821 atagctatcg tgataggttg caaaacgcgc ataactgttt atttcagaat gacagtactt
    23881 ttagtatttc ctttggcatt ttgccattaa aatttgtata tttacttctc cgattccagt
    23941 gcatactttc aaataacgct ttaaccggaa ataatttatt atttttacac cgtaactgta
    24001 ccctatgaac agcattgaaa tctgctttac tccggccctg tacgggcata tcatgactga
    24061 gccgccgttt acagtggcca ttgttgacat tttgcgcgcc accacttcca tttgtgctgc
    24121 cttcgacaat caggtaaagg aaattattcc ggttggcact accgaggaag cgcttgaata
    24181 caaaaataaa ggatatctga tagcagccga gcgcaatggg attaagcttg attttgccga
    24241 ttttggaaat tcgcccgaca atttcaccag agatcatgtg cgcggacgca gcattgtata
    24301 tagcaccacc aatggaacac aaacaattaa aaaaattacc aacggcaatc cggttgttat
    24361 tgcttctttc ataaacttaa aagctgttgc aaagtatata gtcgctaaaa accagaatct
    24421 ggtcattgtt tgtgcgggct ggaaaaacaa attcaatctc gaagactctg tttatgcggg
    24481 tgccttaacg gcagaattac tcaacatggg aatgaccatg aactgtgatt cggcgcaggc
    24541 cgcagttgat ttatggacta ttgcaaaaga taatcccgtt gcctatatac aaaaggccat
    24601 gcaccgcgaa cgattaaaaa agctaaatct cgactcaatt cttgaatact gcttcactcc
    24661 tgatattaca aatgctgtcc cggtttttga tggttacaaa attttaaatg ccacgttgaa
    24721 tgaataaatt tctgaaatcc attaccgtca ttatcgtcct gttttgtctc cctctgggag
    24781 ccaatgcatg gggatttttc tgtcaccgtt tgattaacaa acacgccata tttattctgc
    24841 cgcccgaaat ggtcggattc tacaaaaaac acatggacta cattgttcaa cattctgttg
    24901 atcccgataa acgcagccat gcggtggaag gcgaagctga aaagcactac atcgatatcg
    24961 atcattatgg ggataaacca tttgaaatca tgccgcgtca atggaaacaa gccgttgaga
    25021 aatacagtga ggatacttta cacgaatatg gaattcttcc ctggaatatt gaatggatga
    25081 tgtatcggct caccgatgca tttaaagaag gagatgtgga taaaatagtt aatgtttcag
    25141 ccaattttgg tcattatata ggcgattgcc atgttccgct tcataccact cagtattacg
    25201 acggtcgcaa accttatcag aaaggggtac atgcattgtg ggaaacacgc ataccggaac
    25261 tccttgcaat aaattatgac ttcttcattg gtcgtgccgg atatatcagc aaaccgctgg
    25321 ataaagcatg ggaactggtt gaatacagtc acaacgaaat ggatagtgtc atcaacatgt
    25381 atgactctct gtttctgaat tatgatcaga gcagcatttt tgtgatggaa gaacgtggga
    25441 gtgtcgtttc aaaacaatac agcatggttt tttgcaaact catggaaaaa gaaatggagc
    25501 aaatggttta tcgcaaactt gtgatgtcaa tctattatgt agcttcgttc tggtacactg
    25561 catgggtcaa tgccggccaa ccggatcttg ataaactgat gagcaaagaa gtttcaaaag
    25621 aacatcagaa agaaatggat gaactcgact atctgtggaa aaacggcaaa ccaaaaggcc
    25681 gtccaaatcc ggaagacgag gagtgaaaac actgaacact gaacatcaaa cactgaacac
    25741 cgaacatcga atatcgaata tcgaacactg gacatctaat atcatacact gaacctgtct
    25801 gacggtataa ctgcaacagg cagtcaggca ggcaaggaac atggaaatat gaaggaagaa
    25861 gtaaagtatg aattagcatt aaccgatcac cataaaccaa taaccctttc gaacaccgaa
    25921 caaggaacct gtctgcaaca ggcaggcaag gaaggtagaa ggcagcagtg aggtttctga
    25981 tttaagaaca aggatttttg atctatgatt tttgatcgga aggacgattc gaagactgga
    26041 caaggaacaa ggaaatttga aatgacaaat gccgctctac tgcgtattaa gcaaaagctt
    26101 gattttcact gctcaccgtt caccgctgta gatgcagcca ggtttggcgc acaagataat
    26161 cgtcactgat aaataaaaaa aaccggactt tcatccggtc ttaagtgtat tattttcttt
    26221 ctttcagcaa gtctctgatt tcggtcagca gttcctgatc ttttgttggt gcaggcggag
    26281 cggcaggggc ttcttcattt ttctttgaga gattattggc cactttcaca atcacaaaca
    26341 cacagaaggc aattatcaga aaatctatta cattctgaac aaacatccca tagttcaaag
    26401 taactgccgg cacatcacca acagcatctt taagaactac agaaaggtct ttgaaatcga
    26461 ccccacccat caatattcca atcggaggca tcagaatatc attgacaaaa gaggtaacaa
    26521 tctttccgaa agcagcccca ataattacgg ctacagcaag gtcaagtaca ttgcctttgc
    26581 tgatgaaatc acgaaattcc ttaaacattt tcataggttt ttatttttta gaatttatac
    26641 gaaaatccaa gtccgaaaac ttctttaaac tgtgtgcgcg ggcctttacc aatgacgatt
    26701 ccggcttcat cttttatttc aatatcaatg tcatcgtcgt atatcaatgt tgttccaatg
    26761 gtaacagtga tgtatttatt cactttcata ccaatattgg tttcccagta aacatcgata
    26821 ttctgcgggt ttttaagata attggtaaac aactcaagct tggatgacag ctttacattt
    26881 ttcattatat cctttgtaaa tgcgcctttg aaataacctc caaactcggt ccgcatatgt
    26941 ttgccggctg taagaatagt gctatcgagt gggtttatca ctgcgggctc aacaccgaat
    27001 gcacctgcat tggccagcgt ttcatccaga acaaaagtca ttttgccggt aacaggagca
    27061 agaaacaaac tgagccaatc accttttttg aaatcataac cagcagcaaa caaaaggtag
    27121 ccaggtgcca taatacgtga aatagcaaca gaatcgttcg gataattata tcccggcata
    27181 aactgcgttt taaagttcag caaagctgca gcaaaactat tggacgataa tttacgtccg
    27241 aatttcgacg aaaaatctat tttatcatct gttttccgaa cttcctgttc tccctgtttc
    27301 ataattccat agccaagatc gagcgtatta tcccacgaaa tctttccttt tttataattt
    27361 gcaaaagaat tgacaattga attgccactg aaggaattct cgcccccggc agcccaattg
    27421 ctgagagaga cctgactaaa agcaacggta aacattccac cttttttcca gccctcggtc
    27481 gtgtcggcat tcaactttcg gagatcggac tccttatcag taacctgagc aaacatggtt
    27541 gctgaaatca acataaaagc aacaataaaa aaatgtttca taagcgtctt tttttttaaa
    27601 gtgaaacggc tacccttgat aattcaaaga tagccgtttt tgaaaaactg tttatcagaa
    27661 attgactttg aattcaaccc ggcggttttt ggcacgaccg gcaacagtgg cattatccgc
    27721 taccggaacc gtttcgccat aaccggttgc agtcatccgg gcggcatcaa tgcctttatc
    27781 ggctaaatac ttcttcacag cattggctct gcgttgcgaa agatcgaggt tagcagcatc
    27841 atcgccaacg ttatccgtat gaccattgat ttccaacagg tattccggat tatcctgcat
    27901 taccttaact acattattta aaatagtgaa cgaagtagga cggataatgt cttttcctgt
    27961 ttcgaactga ataccggtaa gggcctgagt gaaaatcttc ttcacttctt ctttcacttc
    28021 agggcaacct ttattggcca caacacccgg tacatccgga catttatcat caatatcagc
    28081 cactttatct ccatcgcggt caggacatcc tttaaactca gcagtaccag cgactttcgg
    28141 acagcggtct tcactatcaa caataccatc attatctgta tcagggcaac cctggaattt
    28201 ctcaataccg gcaactgaag gacaattatc ctcgctgtcc tgaattccgt cattatcggt
    28261 gtcggggcat ccctcaaact tagtaatacc agccacatcg gggcatttat cattcttgtc
    28321 ggcaatacca tccttatctc tatcgggaca gcccgccaga ttgcttaaac ctgccacgtc
    28381 aggacattca tccacattgt ccatcactcc gtcaccatca ctgtcggcag gacaaccttc
    28441 ggcattaact acagaaccag ccggagaatt gagacatttg tcaaacttat ctttaacacc
    28501 atctttgtcg ctatcgcatg gagcaccaaa gccaattaca attcccaccg aatgtttcac
    28561 atagcaatcg ttcatatcgg caacggcaaa atccctgtca tcattaaagg taagctgata
    28621 agttccgata tattgaacag ctattttggg actgatctga tacttgagtc ccaatccaag
    28681 aggcacgata aaatcaacgc cggaaactga gatgcaattc ccattgtaat aagcaagtcc
    28741 tgcaccggca atcagcgatg gagaaaattt ccatttggcc ggaaacaaaa tatcattgtt
    28801 caatttcaaa gacaacaaca aagcaggatc aagtttcttt ccaagaaatc tttctgccga
    28861 agatttaaaa tagccataat cgccataact ggcagaaagg ccgagatcga atgaagggct
    28921 gagataacgg ttcaactgca tagcgccgaa tccgtaaaat gctttgctga aatcaccgat
    28981 ggcatttcca tagtcgccgg tatattcatt tttgccccca aaaaaaccaa ttgaccattt
    29041 gttttctaca gtctgagcac tcatcatctg aaaaaacaaa agacttatca ataataatcc
    29101 tgtaacctgt ttcataatat ttcaatttta atatttatga caaagatagt gcttaacgga
    29161 ggtatattgc aactacattt ttgttaaata tagccatctg taatatattt gaagtaattt
    29221 acataaagta ccaggaaatt tgtctgattt ttaagactta agcagtagaa acatatgaca
    29281 aaatcctata tttatttttc taaaaatcat acatcaacgg cgccccagca tacgcacgcc
    29341 tctgacaata atctgaacat cgttccaggc cttataatcg cgggcataca cgatgttcag
    29401 attgtcgata gtagccggat tatcaatcgg tttccgtgca taatcagccg gattgagaac
    29461 cccttttttg atggccggaa aacgatggct cagcggaatg gaaagattgt atccaaccca
    29521 gcttttacaa ccaaaaaaga ccgaaaagag attggcaatg aaaccaatgg gcgattgaat
    29581 aaaaaacacg agcactggtg aaaaaatcag taaaacaaaa gctccaccaa ggtcaacaag
    29641 tcttttacgc cttaaatttt caggtttgga aatattgtta atgttgtaaa cataataatc
    29701 acctggctga ttgatattat tgctaccaat aatatacaag ctttcagggg gagcaatttt
    29761 aaaatcaatc gatttttggg aaaagcccga cataagactg atgatatcct gcgatgaaat
    29821 attttttgca caaaaaatca cctcattgat gcgataaata tgcaccagct cggtcaatgg
    29881 cgaaataaaa ctgctgccgg cattttgccc aagcacatct gccggattta caaagtggat
    29941 atagtctatc tccactccgg atttttcgag tatggaacta accctatgca cctcctcatc
    30001 ttccccaacg ataacaacac gctttgactg actacccgac aatgtgaatc catctgtttt
    30061 tataaaatga aaaaacaaac ggctcagcat ataataaatc attgtccagg cggcacccag
    30121 caaaataatg gcacgagaaa aacgatattc ttccggcaat aatgaatatc caattaggat
    30181 taagccagta ccaataatag ctccctgagc cattttgcca agtttaaccg gtttatcata
    30241 ccccccattg agaaagactg aaagcatcca tagaaaagta taaacggcta ttcctatcgt
    30301 caataattca actggaaaag cacccccgtc agggaaaatc acgttttttt gccagaactc
    30361 actgatcaga tacagccccg aaccaattaa agcagcatcc agcagtggga ggaaaatttt
    30421 tctgataaag cgcgccatga gtgccaaacc agctctgaac catatagcca tattaataat
    30481 ggcaataaac aaacgcagtt tttcgcgtgc ataatgcttc cgcgcaaaaa taatcatcgc
    30541 attataaaat acaaatacat aattaacact gctttttttg gtgctctcgc ctttataatg
    30601 aataatgcgt gtttcaggga aataatagtt tttatacccg gcttttaaaa tgcgatacga
    30661 caaatcgatg tcctctccat acataaaaaa agtctcatcc agcaagccgg ttttatccag
    30721 tgtttcgctc cgcaacaaca taaatgcacc actcagaacc tctacctggt gaattttctg
    30781 tttgtcgaga taggacagat gatatttgcc aaaccgcttt gagcgcggaa aaagcgatga
    30841 tagtccgaaa attttataaa aggagacttc cggtgtgggt agtccccgct ttgattccgg
    30901 aagaaagttg cctttgccat ccaccatcat cacacccagt ccgccggcat cgggtgtgcg
    30961 atccataaaa tcgatcacct tgatgaatgt ggtttcctcc actactgtat cgggattcaa
    31021 caacaaaaca tattctcccc tgctctgcct gattgcctga ttgttggcct ttgcaaatcc
    31081 cagattctct ttgttgtcaa tcaaaataaa atcgggatat ttattcttca ccatctgcac
    31141 cgagccatca acactgttgt tgtcaacaac aaacacttca ccgtcaatgc ctttcatggc
    31201 cagtctgact gaagccagac actgctcaag aaaatgctct acattgtaat tgactataac
    31261 aaccgaaagt ttcatcagag caaaaatact gataaaactt taatgcggaa taaaacaaac
    31321 aatttatcag tgatgtttac gcaacttcag gcgcagtgcc accgaaaaat caatattgaa
    31381 atacattccg ccgtcatcgc tttgaaacgt tgactcatcg taacgaatga catcattatg
    31441 ctctgagaat tgacgcagac tcagttcgca cgaaaacgac aaaccaactc tgcgcttgga
    31501 tgaataaccc atcctcaaac caatcccagt cactgttttg tcatgccaat aagtcgaagg
    31561 tctgctttgt gtcgaaatat aattggactg atatactttg atgctatcgc ccataatctg
    31621 tttgaaataa agcgaagggc cgatgaaata gaaacagctg gaatcgctcc ggttggtgaa
    31681 tgtgaaaacc ggattcagac ggaacataat gtggctgctg atgcagtcgg gctcattcac
    31741 acggcggacc gaataaacat atccattcgg cgacagcggc ttgtagccgg cctgcagcgc
    31801 aattccccag ctgggtctga aataattgtc gaagttgaac gtgaaatttg gaaccggcag
    31861 cttttcgaga tcagtcccga taaagcaaaa ctccgatgga tcctcactcc gaagtaaacg
    31921 tttttcaaac cgaatgctat cttttgccag tttcttcgcg ttttcttttt tttcaggtgt
    31981 tttgtttgtg tcttgtctta gatactgcct tattgaatca ctttgcagca aagccgttgt
    32041 ttctttcttt ctcatccgat tgataacaac aaaatcatcg cctttgactc tggtggtaaa
    32101 ttcctcataa ataaaagcac ccaatgtaat ataaaaaaac tgatactgaa taagcagcaa
    32161 gggcgtactg cacaaagcaa taaaacccgg agtacaatac ttcaatgtga aaacttctgc
    32221 cggattaatg ctgtcattac caaccaaaaa caaattttca gaatgtagtt tgatagcacc
    32281 aaaagagtgc ttgccttcag tcgttacaac tttcacccag tgtccgttgc ggactaccca
    32341 actgattttg tgggaggtgc cgtcaacaag gtgcattctc agcggttttt gctcctgagc
    32401 attcagcagt attgctgaca taaaaaacaa ataaaccaat agaaaacgca taattactta
    32461 tacttgaaca aatgcaaata taagcagtta tttaactaaa aaatacattc gcgaaaataa
    32521 ttaaaacggc aggtcgtcgc cttcgtttac tgaaaggtca atgccacccg aagttggtgc
    32581 attctgcgcc acatcgtaat cgggcatacg gtcctgcata tcgccaccgg ctttttcaac
    32641 tttccaggcc ttcacgctgg tgtaccagcg accattgtat tcgcggcttt cgatgtcagc
    32701 ttccacccgg acttcttcgc cctgtttcac attgaactgt tccactttat cgccaaaaat
    32761 ggtgatgcat atttttttgg gatactgtcc cggtacttca agaatgaaat cctgacgctt
    32821 ccatgtgcca ttctgtccct gaccgctcac tgcctcgagc acctgaataa tttttcctga
    32881 taattccatt atagtttctt ttgaacaaaa atagtaaaaa tcgtttttcg gattacaaca
    32941 aatgtgtttt tttattcaca attgaaggtc ccttactttg gcgcgaccca tgcttcactc
    33001 tggcgcggat ttgcaatccg tgacagtgta gccatccaac gccttcaacc gctccagcac
    33061 aggcgggtgg ctgtaattca taaacacata atacggatgc ggagtcgggt tgctcaggtt
    33121 gtcaaccgac agttttatca aggcatctgc cagcgcaggt gagtgcgtat gctcccatgc
    33181 aaagcggtcg gccgcatatt cgtgcctgcg cgaaatcaca ttggagacaa ttcccatgat
    33241 ggtcgaaacc ggactgaata ataaggcgaa aaccagcaga gatgcgtgca gcgacggctc
    33301 cattccaagc gcgccggcaa actccggttt ggatagaaac agattgagca gatacatgat
    33361 cccgagattc agcactgtgc tcatgaaaaa accggtggtc acatgtttca ttttgtaatg
    33421 accaatttca tgcgccagca cggcgacaat ttcttcggat gtgtgttttt caatcagcgt
    33481 gtcatacagc acaatgcgtt tcttcgggcc gatgcccgtg aaataggcat tcgattttgt
    33541 agagcgcttg ctgccgttga tgacgtaaat atttttcaga ttaaagccgt ttgttttagc
    33601 gaaaaattca atggcatcgc gcaattcgcc ggctttaagc ggtatttgtt tgttgaacag
    33661 cggcacaatg atgttgctgt aaaacatggt catcagcagc gaaaacaacg tgagcacacc
    33721 ccaggccagc agccagaaat aatcagagat tgaataataa aactcagcca acaaccaaaa
    33781 aatgccacca cctaaaatgg ctgacatgat ccatgatttg aatttatcgc cgataaaaat
    33841 ggccggcgtt gatttattga agccgtattt ctgctcaatg gtaaaaacag aataccattg
    33901 aaaaggcaag gtcagaatat cggcactcaa aagcagaatt ccaaaaaata tgattgaatg
    33961 cccgattgaa tgattccata ttcccgcagc cagattatcg atccaaccaa aaccaccggc
    34021 aaaaagcata gcgagaataa ttgccagttg aaaaaatgaa acaatgtgtg aaagtctgct
    34081 gcggtccatt gagtaactgc gggctttcag aaatttttcc tgagtaaaaa cagaagcgat
    34141 tctttcagga acagattgtg gccagtttct ggaattcagc actgaaagcc aggtatcaag
    34201 gatactgtca aacagaagaa atgcaattat cagaataaaa atcagatttt ccatgtaaaa
    34261 accaatcagg atgggagact tgtagtatca gccgcttctt ctatttgaac gcctgcagga
    34321 acatcaaatt tttgtgaagg aatttcaaca tttactttca tcttcactgc ttccatagag
    34381 acttcaatgc ctgaaaccga cgaaaccgac tttaaaggaa taccttccca tatccagact
    34441 ttcatgtcta tttcctgtcc attcacattt tgtttctggt tgtagatttt acattctttt
    34501 tcaagaaatt tttcagatcc ttcaaaaaca atattgtaac gtttcatatc atctgcagaa
    34561 agggttctat agttgtagtt agcttccgag ctgtcctgtt ccattgtcat ccgagagcca
    34621 actttcagaa ccatatcaat attataaacc attgaatcct tcacaatatt caattgatga
    34681 accttctgac caaggactgt ggatttgatt tcgctaagtg ttttacggcc aaaatcgtcg
    34741 aaccacatgg taatttcctg cgtgataccc atcgtagatg aagtgtaaac aataacgcct
    34801 gttttaaagc caaactggtc ggaatcggaa ggagccacag cggtaatttc cttaagagaa
    34861 tcgagcatgg acgccacttt ttcctgatcc ggtttattgc cacaagaaac aaaaaagaac
    34921 agagagagca ttgagagaat caaaagattt ttcatattca gtttaaatta aagtcggtat
    34981 ttggtgtgca gcgaaacaaa agaaaagata tcgcgagcaa atattttaaa atccgaatcc
    35041 atctcattat aatatttcac aagatattca acggtatcgg gcaaagcatt ggcaaaatga
    35101 gtccgcttgg tcactccaat aaaaatttca ttaagaccat caaaagtttc atacttaccg
    35161 aaccaattgt cctgcattag tttatgagac actttctgac ttttaggcgg caaagtatcc
    35221 attttttcat gcaacaatgc ataaaaagca ttgatttttt taatcattgg cgttttaaca
    35281 agctgcttcc agtttttaat cagaaaatga tcaaataaaa tatcgacagc cacggtagca
    35341 taacgggcat gagagggctt aagcaatgca acgctgtgac gcacagccgg atgcatatcg
    35401 ctatatgcat caatagccct atgcagcaac acacctttta aaacagggtt tggcaattcg
    35461 gccataatta tggttcccct gatatggtcg gcaatataat ttccaatgat aatatccgga
    35521 ttatcaccac ttaaaaccag atgaaacaga taattcatga tgtaaaaata attcaaaaat
    35581 gttttccttc gccaaaagaa aaaaaataat tattttcgtt gcagaataaa aagaaaagca
    35641 aaatatgcaa aaacagttat tattaggcga tgaagccatt gccgtaggag cgatagatgc
    35701 gggtatttcg ggcatatatg cctatcccgg cacgccctct accgaaatca cagaatatgt
    35761 tcactcaagt aaggaagcac aacagaaagg aattctggct tcgtggggtg ccaacgaaaa
    35821 aacagccatg gaaactgctc tgggaatgtc atatgcagga aaacgtgcca tggtatgtat
    35881 gaaacatgtt ggattaaacg tagctgccga tcctttcatg aatgcagcca ttaccggggt
    35941 caatggaggt cttatagtgg tctcggccga cgatccatcc atgcattcat ctcaaaatga
    36001 gcaggatagc cgcatttatg gaaaatttgc caatgtgccc attcttgagc catcgaatca
    36061 acaagaagca tacgacatgg tgcattatgg atttgagctg agtgaaaagt ttaaagttcc
    36121 tgttctgatg cgcattacca cccgtctggc acacagccgc gcaggcgtag gccgcattga
    36181 aggccgcaaa caaaacgata tgtctttgcc ttccgattta agacaatttg tactactgcc
    36241 tgccattgca cgcaaaagat acaatatgct cttaggcaat caatccggat tccgaagcga
    36301 aagtgcctct gccggcttca atcaactgat cgacggcccc gataaatcaa tgggtatcat
    36361 tgcctgtggt attgcctaca attacctgat ggaaaatttt ccgcagggat gtcctttccc
    36421 tgttctaaaa atcggtcagt atcccctgcc tgttaatctg gtggaaaatc tggctgatac
    36481 tactgagcaa atcatggtgc ttgaagacgg atacccaatg gttgaagaac tccttgcagg
    36541 gctcctccag cgtggcatca aggtttcggg ccggttcgac gaacgtattc cccgcacagg
    36601 cgaactaaac ccaaatatcg tcgcacgggc ttttggaatg gccgatacac acggattacc
    36661 ctcacccgaa atcgttgttg gccgcccacc ttccatgtgt gttggttgtc cgcatatcga
    36721 cagctataac gccatgaatg aagcactgct tgattattca aaaggtcgtg tattcagcga
    36781 tataggatgc tacactcttg gtgcacttcc tccattcgag tccatcaata gctgcgttga
    36841 tatgggcgct tccatcacca tggccaaagg cgcagccgat gcaggacttg ttcctgccgt
    36901 agctgtaatc ggcgactcaa cattctgcca cagcggaatg accggtctgc tcgatgccgt
    36961 tattgcaaaa tctcccatca ctgttgtgat tctggataac tcaacgacag gaatgaccgg
    37021 tggtcaaaat tcagctgcat tcggccgagt aaaagacatc gtaaccggcc ttggcgttga
    37081 accagagcat atccggattt tgaaacccct gaagaaaaat cacgaggaaa acacgcaggt
    37141 tttcaaagaa gaacttgcat acaacggcgt ttcagtgatt atcccaaccc gcgaatgcat
    37201 tcagacactc aaccgcagaa tgcgcgaaaa attcaagaat aaagcaaact aatcagaaac
    37261 aacaatgaaa aaagacatca tattatcagg cgttggcgga cagggaatcc tctctatagc
    37321 tgccaccatt ggtcttgcag cagtcgataa gggctggtat ctcaaacagg ccgaagttca
    37381 cggaatgagc cagcgtggcg gcgatgtgca aagccatctc cgggtttcgg aaaatgtaat
    37441 tcattcagat ctgattccca tgggtcaggc cgatatgatt ttatcagtcg aaccaatgga
    37501 atcactgcgc tacctgcctt atctgcacaa agacggatgg cttgtaacca attcaaaatc
    37561 ctttatcaat atttcgaatt acccggatat agaaaaagtg tttgccgaaa ttaaaaaaat
    37621 taaaaatcat atcctcattg atgcagatga aattgcagcc gggctgaatg cacccaagtc
    37681 ggccaatatg gtcatgctcg gagccgcatt tgcctttctt ggaattgaaa gaaattattt
    37741 cgaaaatgca atccgtaatc ttttcggacg taaaggcgaa gaagtggtga atatcaatat
    37801 caatgctttt aatgcaggcg ttgaatttgc ccaacaatac attaaaaagt aaattccaat
    37861 acttttcaat cttttttttc atcaaggcac gctttcgggc gtgctttttt atttattgat
    37921 tgtcaacaac atatccgaca acaaacaatt acaaacattt atagtcgaaa gtaagtactt
    37981 attaaccgac tcgtacaaaa aggtcaccgt tactttgcac cgtcggaagt caatcagtcc
    38041 ggcgaactca aaaaactttt taaacaactt aaaacttaca cgtatgaaaa agaacaatga
    38101 aaaaaagatg gttgaactca acgaagaact tgagaacatc gaagtaatgc aggaaactgc
    38161 agacaaaaac gaaaactcag gacatcgcga gatgaatgta gtggaattgt gcggatatgt
    38221 tggctttgat ccggaggtgc aggatttcgc cagcggtaaa aagaaaatca gatttaaact
    38281 tgccacacac cgcaccctcc gcacagatgg cgaaggcgaa ggtcagaaag tgaccaactg
    38341 gtttccggta gtggtatgga ataaaactgc cgatgaggct ttgacatggc tgcgcaaagg
    38401 aaacaaagtg aaaatcaaag gttatctgaa ctaccgcacc tgggaagaca aggacggcaa
    38461 caaacgcaat ctgtacgaaa ctgtagcaac tgagctgtct gccattcctg cataattgct
    38521 tgctttaatt gggtttaagc gctgttccgt aaaaggggcg gcgctttttt aaaccataaa
    38581 aatgcttaaa aaactgtttt atgaaaaaag aagaaatcac agaactcttc aataaatttg
    38641 aaaatgcctg ttatgtatat caggatattg agtgctggag cgccagagag cttatggaaa
    38701 tactcggata tactaaatgg caaaacttcg aaaagataat tgataaggca atccaatctc
    38761 tggaaaacgc gggtgagact gccctagacc attttactga cgtcagtaat atggtccaaa
    38821 taggctctgg gggcgtaaga gcgctttcag atattgcgct aacccgctat gcatgctatg
    38881 ttgtggcgca gaatggcgat cccaataaac ccgaaatagc attcgcacaa acctatttcg
    38941 ctgttcaaac aagaaagcag gaattggttg aacaacgatt attagatgta gccagagtta
    39001 atgcaagagc aaaattgact aagtcggaaa agaagctttc cgaaattcta ttcagtaaag
    39061 gaatagatga aaaagggttt gctcatatcc gttctcaggg tgacaaagcc ctgtttggtg
    39121 gaaaggccac tcttgaaatg aagaagaagc tacttgtacc tgattccagg cctatggcag
    39181 actttttacc aaccttgctg atcaaagcaa aagattttgc aacagaactc accagtcaca
    39241 acgttgtaga gaaagacctg gaagaatgct gcgctatcac tgaagaacac atcaccaaca
    39301 acacatctgt cagaaaaatc ctgctgaaac gtggcgtaaa gcctgagaat ttgcctccgg
    39361 gagaggatat taaaaaagtc cagcgacgac ttgacactga aacaaaaaaa gtctgcgcca
    39421 atacgaaaaa aattaaacct cgaaaaaaat aatcagctct tatttcaaaa ggcctttccg
    39481 gaagaggaag ggctttttta tatatgatca tttgaattcc ccacttcaga ctccgctttg
    39541 agtactcttg acaactcttt ttgaatcctt gacttctgtg atgaatagat cagtccggca
    39601 attaaagaca acagtgaaaa tgttataaaa atatcctgaa tgtcgaatga gttggccagt
    39661 gtaccaatga gaatagctcc cagtggtgtt accgtcatga acgacattcc ataaaatgaa
    39721 atcactctgc cggtcatatc gggcttggca attgtttgca acaaggtatt gacagaaaca
    39781 tactgagcaa tcattgccca gccacacaaa acaagcaaag ccatactcaa tgccgtaaca
    39841 gtcgaacagg aaaaaagagc aagccctcct gaaaacacaa aagcagctat cattatgtac
    39901 acaggaaaac ttttcagaga cttccgtgat gcaagaaaaa acgcgccaat cagtgcgcct
    39961 gcgccataag aaccagtcaa tacgccataa agactactgt caccattaag cacatctctt
    40021 gcaaataccg gcagaaaact ttgaaacgga aggatgaaaa aaccggtgga aataaccaat
    40081 atcagcatgt acctgatcgg agcaaaacca aatgaatatt ttattccatc ctttaaatcc
    40141 tcaataattg tatttgtatt gttttccttt ggaataaaac caactctcac tgcatataat
    40201 gacgcaataa ccgctgtaaa actgactgca tttatggcaa aacaccatcc ttctccgata
    40261 atcgcaataa gaattccgcc aatcataggg ccgacaaatc tcgccgtatt gaaaagcatc
    40321 gaattcaacg caatggcatt cgacatctgc gatgtgtctt ccaccagatc gcggatgaag
    40381 gcatggcgaa atggcgtgtc gatggaattg acaatgcccg aaagtgctgc caacgcgatg
    40441 atatgccaca cttcaatgtg atcagaaaga acaagtatag ccagtgtcag tgacagaaac
    40501 atggacgcat actgtgtgaa taaaattacc ttgcgccggt tcatgcgatc ggcataaaca
    40561 cctgctatcg gagttacaat aagcgatggt atctgacctg caaaagtcac aatccccagc
    40621 ataaaagcac tgtctgtcaa tcggtacacc agccagccgg tggcaacaat ctgaatccag
    40681 gtgccgagca ccgaaaacaa cattcccgaa agatatatgg catagttttt cgaccgcagc
    40741 gcacgaaatg tgaacgttgc cctgaacaga taattcttta tttcggacca ggggaggatc
    40801 atgccgcaaa agtaataaag ttgaaagtac ggacggttga attaatgcaa aatcatttta
    40861 tgcaaaaatg aaatatataa cagtttaatt tcattggatg actccggagg agtcacatgt
    40921 ctgtagaaat acatctgaaa aatcaacgac tccggaggag tcgaatgttt tgaagctaat
    40981 tgatgatgcg atgtctttta cattcgacct ctctgaggtc gttgctagta tgtgcatatt
    41041 tctacagaca tgcgatgcct gcctgttttg tcaggtaaac ctctggcatc aatctacact
    41101 caaacacatt ggcataaaat atatttcaca atggtattac tgcatcgctc cgcgcgcttt
    41161 cagaatccat tttgcttcgt cgagcggact gattcctttg tagggtctta ctactccaag
    41221 ttcatcggca cagggacgat acccggcaaa ttgtgtcgaa atctccgctt ttccaccact
    41281 gcgcgaacta agttttacag catagtcatg cgatgtggaa acaggcagaa aaccggtgag
    41341 cgtgattttc ccattatcca tttcaggact gtcgaaggta ccacgcattt ttgtaatgtc
    41401 gcttgttatc agcccgagaa gatcttctgt ggctttgatt ctgaaagtga caaatggctc
    41461 cagtaatgtc gttccggaat tcaccaatcc gttcatgatg cccatgggcg ttgcaataat
    41521 aaaatctccc ggacgcgaat gtatttcatg atcctcgcct tcaatcagtg taatgcgcaa
    41581 atcggtcact tcccagccct tgataccctg tttcagtgcg gccggaatgg tttgttccac
    41641 ttcgttctga tatttctgcg ccacatcatc aaccctaact tcactccggt aaacaacgcc
    41701 gcttcccggc tctccgggct caatgcggaa tttcagaatg gcccagcagg gtttgggcat
    41761 ccagtagcgg acaaatcctt ctgcaatgga tgaaggcgtt tctttatata tgaccgttgg
    41821 ctgttcaaac gtggccgcaa agccgaagcg atcaagcaga atccgctcca gcacttccat
    41881 ctgaatccag cccatcacac tgatgttcat ttctttttca tcgcgaagcc actcaaaatt
    41941 cagtgacggg tcttcgctgt aaagctgttg taatgcggcc gacagcggac tgtaatcttt
    42001 ttcattcaat gctttcacac gaacagtcag caaaggcatt tgcaatggtt taagattgga
    42061 tgtgtctaaa acgctctctc caagccagtc tccaaccgaa gctgcagaaa atccgcacac
    42121 gcctgcaatg tcgcctgcaa caacctgctg caaatcttcg aaacgcattc cactcatacg
    42181 acgcagctga acaacttttt catcaactga ttgcgtggca ttccggatca gttcgcggtt
    42241 actgatgctt ccgccaaaca agcgcacatg cgccactttt cccatcgttt tatcgtagct
    42301 gatgccaaaa actctggcgc gcaaaccgtc gacggcattt tcatttgggg caggaagaaa
    42361 tttaatcatg acatcgagca gctgatcaat tccaatattc agccgtgctg aaccaaacag
    42421 catggggaca atctcgcttc gtgaaaccat gtctgcaagc tgtttttcaa gtatttcaaa
    42481 atccggcatc actccttcga ggtaagcgtc catcagtttt tcatcacccg caacaatagt
    42541 ctcagcaata ttttccgcaa tatggtcttt tgaaaataaa tccgtaatga caacatctgt
    42601 gctggcaatg ttctgaaccg actgcatgca aagcggctcc agatgccatt ctttgcgtat
    42661 atccatcaaa acattttcaa tgtcggcgcc caagcggtca agcttattca aaaataaaat
    42721 cacagggata ttgcgcgcac gcaaagcatt ccacaaagta ccggtatgtg cctgaatgcc
    42781 ttcggctgca gaaatcacga gtacagcacc gtcaacggct atcagaatcc gctccacatc
    42841 ggccgagaaa tcaacgtgcc cgggcgtatc gaccagattt atcctaatgt ctttccataa
    42901 aaaagtagtg tgagccgaac ggaccgatat gcctctctcc cgctccactg cgagaaaatc
    42961 agtatgtgtc gtgccatcat ccacgctgcc gggatttttg atggcaccgc tgtgatgcag
    43021 catcaactct gtaatactgg tctttcctgc atctgcatgg gcaaggatgg ctatgtttct
    43081 gatgttttct gtcatcgttt cgttttttgt gtggttaatg ccggattgtt caaatacttt
    43141 tcgccgaaat actgcatacg gaaccacagt tcaaatgcgg gatccagaaa aacatgtttg
    43201 ccgttttcca gatcaatgat gtcgttttca acaagaatgg ttctgttttt ctgaacattg
    43261 cgcggagtgc cgagccggta tttctgcatc accgaggcac tggtcatttg tgtttcgtta
    43321 cagacaacag ctttgaggaa attcagctgc gtaacactga tggattcaac ttccttttga
    43381 tataacggcg aattggcgtt tatgagttct gtcaatgcgg ccgacaattc ctcaattcca
    43441 gccgacttct ccgttttctg ccatgtgtaa tgcgacagtt gctgcacata ccatgaatga
    43501 tcattcatta aaaacggaat tttttccgcc atttccgcag aaatggattt tcctgatttt
    43561 ttaaacccgt tcacaataaa agtaacccag ttttttcgcg gaattttatt cagcagcata
    43621 atgtcgccaa agcgatagaa aggttttgac ggattattaa atatttcagt catcatgtga
    43681 cgtttgctgc catacagaca ataggtcaca tttttctgac gctgccagac cgatctcatt
    43741 tttttctcaa agcttgcaaa gccagcaaat gaagccaggt tctggaattc atccagacaa
    43801 atgatgtatt gttttccttg ctttactgca atcctttccg gcaaattcag cacctcatca
    43861 ggaaatttat tcaactcggt ccagttaaaa ccaattctga aatcagtgtt tggatcaact
    43921 ccaatggtta tctgaggaat caaccgggaa aagaattctt tggctgattt cacccagtcc
    43981 tgccactttg ttgatgaagc catgatgact tcccgcgcaa ataactcgag aaaatcctcg
    44041 gctgatgaag ccgaaaacaa atcaatctgt actgtaattg tatttctgtt ttttgtttta
    44101 ataacatgca gcactttctc aaccagactg gatttccccc agcgccgcgg agaaatgatg
    44161 gacgtgttga ctccttccgt cagattgctg tagagctttt ccgcatcctt ttcacggtca
    44221 gtgaaagaat ttatactgac ggttgttcca taaataaaag gtgtgctttt cataggctga
    44281 agtttttact aaaaggtatt atactattag atattatact aataggtata atactaatag
    44341 gtataataca caaaggtata aattattttg aattcgtatg atgcaggtaa tggttatcgg
    44401 ttaatggtta ttggttatcg aatgggtttt acttctcact tccttgcctg cctgactgcc
    44461 tgatgcagtt aaaccggcag acaggttcct tgttcatgat tcgatagagg gtattggtta
    44521 tcgggtaatt ggttaatggt tatcagggag ttataagtcc gccgcggcgg attcgtattt
    44581 cgtatctcat atttcatatc tcacatttct tatctcgtat ttcgtgtttc atctctcgca
    44641 tttcaaacgt atctcatatt tccacatgcg ccgaacctgt cacgtttcaa gtgagcgcca
    44701 catccgttcg tatttcatat ttcgtatttc atatttcgta tttcgtattt tgtatttcgt
    44761 attttgtatt ttgtatttcg tatttttcac cccttcacac ttccttattc cttgttcatt
    44821 attcttatta tctttgcatc aaattacaag gcatgtccaa ctggatattg atttttcttg
    44881 tgcaactcaa ccgcaccggc ctatttgcct gaggcctcct gattccattt cacatcttaa
    44941 taaaaaacgc agcaccggca tgaccgatgt tgttgacatt tgcaaatcaa acaaccaaaa
    45001 aatgaaaaaa caaacactga tcataaaatc cgaaattcgt tttgctgaca tcgaacctat
    45061 catgctgggg catgttcaag ttgaactttc tgctgccgcg ctgaaaaaaa taaatatctg
    45121 ccggcaatat ctgaatcaga aaattgaaaa atcagatgca cctgtgtatg gcatcaacac
    45181 tggattcggc tcattgtgca ataccagtgt tggcaaaaaa gacctgtcca aactgcagga
    45241 aaacctggtg ctttcacatg catgcggtct gggcaatgaa gtccgaccgg aaattgtgcg
    45301 ccttatgatg gtgctcaaaa ttcaggcatt gtcgctcgga cattcgggtg tacaaaccga
    45361 taccgtacag ctgctggccg atatgtacaa caacgatatc ctgcctgttg tttacgaaca
    45421 gggctcactg ggtgcgtcgg gcgatctggc cccgctggca cacatgtgcc tgccgctcat
    45481 tggcaaaggc gatgtaattt atcagggaaa ccgcatgtcg tcgaaacagg cttttaaaac
    45541 ggttggtctc aaaacggtcc agcttcagtc aaaagaagga ctggcgctgc tcaacggaac
    45601 tcagttcatg tcggcgcatg gggcatatgc gcttgtaatg agcaaaaacc tgatgcaatg
    45661 ggctaatcat actgctgccc tgtcgctcga agccttcgac ggccgcaccg atcctttcca
    45721 tccgatgatt cagtccgtcc gcccgcatcc gggacaaatg caggttgcca acgccattaa
    45781 taaaatcctg aaaggaagta aaattgctgc ccgtaaaaaa cagcaggtgc aggatcccta
    45841 ttcatttcgc tgcattcctc aggttcacgg cgctacctgg gacaccattc aatatgtcaa
    45901 tgaggttttc aaacgcgaaa tcaacagcgt gaccgacaat ccgactgtgt ttcccgatac
    45961 cgacctgatt atttctgccg gaaactttca cgggcagcca ctcgcactgg ctttcgactt
    46021 tctgggcatc gccatgtccg aactggccaa tatcagcgaa cgcagaattt atcagttgat
    46081 aaatggtaaa cacgggcttc caaattatct ggttgcaaac ccgggcctga acagcggttt
    46141 catgattccg caatacacag ctgcttcgat tgtgagccag aacaaacaac ttgcaacacc
    46201 cgccagtgtc gatagcattg aatcgagcca gggacaggaa gatcacgtga gcatgggcgc
    46261 caatgcagct accaagacca tccgcattct ggaaaatgtg gagaaagtac tggccatcga
    46321 actcatggcc gccgcacagg cacttgaatt ccgcagacct gaaacatcat cgtcttacat
    46381 ggaaaaactg attgccgaat accgcaaaga agttcctttc attcaacagg atgactacat
    46441 gagcgtccat atgcacagga gcattgaatt tatgcggaca ttcaaattaa aggctgagta
    46501 aagaacactg aacaaggaac actgaacaag gaannnnnnn nnnnnnnnnn nnnnnnnnnn
    46561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46621 nnnnnnnnnn nnngaacact gaacaaggaa cactgaacaa ggaagtgtga agtgtggagt
    46681 gaatactgaa tcatgaacac tgaacgattt tagaatatcg atcggtgccc ttcgacaagc
    46741 tcgactacaa atttttgatt tttgatcgga agggcgaagt aaagacaaga gtatttgaag
    46801 taccgttcta cttgatgaac attaagaact tgatgaactc tattcgaaca ttgaacactg
    46861 acggacgaag gaatggtcgg tttaagtccg ccgcgaccga cgaaggacga agttgagaag
    46921 tgagcattga atgatgaatc gcttattcaa tttttgtatt ttacattata cattttctaa
    46981 tcatttttgt atttcgtaaa atacattttc cacccatttt tgtattttct aaaatacaat
    47041 ttaccccaga cggcatccca acacgctttc gacacgaaat gccactttga ctgtttgggt
    47101 ttttgtattg attataccaa aataaaatat ataatagtcc gatttgcaat ggtcgactcc
    47161 ggaggagtca tatatctgta gattatcatc acaacaaaca acgactccgg aggagtcgaa
    47221 tgtaatggag tagtgtcttc aaataatgcc tgaatatatg cgacccgcca gccatgtgct
    47281 gatcctgacg aagcaacgtg gatcggtcat caatcttcac tgaaacacat ctgccggaaa
    47341 tatatttcaa aatggtataa aataaatagc tctgcaattt tctgtatggt gaatttctca
    47401 atttcgatac agaatggttt gtatcattta tcagtcacaa aatattagca gcacctgttt
    47461 ccgcctaacc cgcccgactc atacaaccaa gtcccccgta cgatatcgtc agtggggtct
    47521 cctgaaatgt agattcagca agggctatga cggttttccg ggagatcccg gcaccgaagc
    47581 aagagctttt tctcatacac tgaccgggaa ttttctgcgc ctttgattta gcagctttcg
    47641 tttgacaaag gccaagtggc cgttgacgaa ggttttggcg atggtggccg gcagggtttc
    47701 gccttcgagg ggcgaccagc aaatcatcac atttatcagc atcgttcaaa cgctggttat
    47761 cctgaaaatt taagattaat actttttggc gttttaatac tgtagagaaa taagttcagc
    47821 agtccaatgc atgtaaggtt cacagcgggt taataaatat gcattgaatt cagcaagaaa
    47881 atcaggatga aatttttcac caaatgagtc aataattttt gtttcataat tatcaattac
    47941 atcgaccacg cagttgtcaa cgatattctg atcaataggc tgattctttg ttttctcaat
    48001 caacatggct gtgaatgctt ctccaaccag cacggttgtt tctgagagtt ccggaacaga
    48061 atcacaatta aacatcgtca ttctttgaaa cggaacacat tttttcatca gctgaaaaat
    48121 gatttcctga ttcacatgct ttattgcatc agtctcagat aaatccggat tttgtgcttt
    48181 gtaattctta cctattgaaa taatgtcttc tgcatgtgaa aaaagactaa gtgtcagcaa
    48241 aggttttgaa tcacggatta tttcctcctt tgattttgaa aaatcaatag cagacaagct
    48301 gtcacaaaca tcatcggcaa tggaaatttc agtaacctgc gcctgcaaag ccgaaaagcc
    48361 ggataacaac aataatagta ttaaaagatt tctcatacgt gagagccaaa aaatatttta
    48421 ccaagcatta atccctctca aaccccaacc gcttccccac tttcgtaccg acaatttttc
    48481 cgttgtccca gaccaggtgg ccgttgacga aggttttggc gatggtggag ggcagggttt
    48541 cgccttcgag gggcgaccag ccgcatttgt agaggatatt ggatttggtg attttgtatt
    48601 tctttgaagt gtcgaccagc accagatcgg cgtaatagcc ctcgcggatg aagccacgtc
    48661 tggagacatt gaacagaatg gccgggttgt gacacatgta tttcaccaca tcggccagcg
    48721 accacaaacc cgattcgctc atggcaatca tggcctgcag actgtgctgc accataggtc
    48781 cgcccgaagg cgcatcaaaa taaggtttca ttttttcggc taataaatgc ggcgcatgat
    48841 cagtagcgat cacatcgagc aaacccgctt tcagtccttc gcgcagcgcc ttgcggtctt
    48901 cggcggtttt gatggccggg ttccatttca ccagattgtc gtgcgtgcgg taataatcgt
    48961 tggtaaacca caggtgatgc acacagactt cggcggtgat gcgttttttt tcaagcggca
    49021 ttccggcatc gaaaagttcc agttcctttg ccgttgacac atgaaaaaca tggagccggg
    49081 ttttcttttt gcgcgccagt gccaccgcgt acgacgaaga tttgaagcag gcttcggcac
    49141 tgcggatgga aggatgcacc gatgcactga tttcgtcgcc gaaaaagtct ttaaaggatt
    49201 ctgcattccg gcggataatt tcatcgtcct cgcaatgcgc agcaacgata tgcggcgtct
    49261 cagcgaaaag ttgttcaatg gttttcggat tatcgaccaa cagatttccg gtagatgaac
    49321 ctaaaaatat tttcacgccg caaacggtat ccagtggcgt tttcagcact tcttcgatgt
    49381 tgtcgttcga cacacccatg tagaaactgt aattggccag cgaatctttc gaagcaattt
    49441 tatatttgtc ttcgagcaga tcctgcgtga gtgtgggcgg tttggtgttg ggcatttcca
    49501 tgtacgatgt cacgccaccg gcaacagcag cgcggctttc ggagtatatg gtggcttttt
    49561 cttccatgcc cggctggcgg aaatgcacct gatcgtcgat gacacccggc aacagatatt
    49621 gtcctttggc atcgatggcc gtatagccgg cgggagtttt ggaatttttt ccttcgttga
    49681 tttttttaat caatccaccg gaaataaaaa cttcgccctc aaagatcctg ctttcgttga
    49741 cgatccgggc gttgaagatg tgataagatt ccattactgt tttttatgac ggcgctttgt
    49801 gttgcgcaaa cgtagctgaa taacaccaaa aaaagcttct cgtataattc ccttagtcat
    49861 tttcgactga cccaatgtgc ggtcggtaaa gatgatcggc acttccttga tggagaaacc
    49921 aagtttatgc accgtatatt tcatttcaat ctggaaggcg taaccgagga aagaaatttt
    49981 ttcgaagcgg atggcatcga gtactttgcg tgtgtagcaa acaaaacctg cggtagtgtc
    50041 gcgcactttc atgcgggtaa taaaacgcac ataggccgaa gcgtagtagc tgagcagcac
    50101 acggcccatc ggccagttga cgacattcac gccggtgata tagcgcgagc cgaccgacat
    50161 atcgtagccg tcgtcgtggc aggctttata taagcgaatc aggtcatcgg ggttgtggct
    50221 gaagtcggca tccatttcaa aaatatattc atagccatga gccagggccc atttgaaacc
    50281 gtgaatataa gcagtgccca gcccgagttt acctttgcgt tcttcgatat gcaggcggtc
    50341 attgaattca gggattaaac cttttacaat atcggcggtt ccatcggggg aattatcctc
    50401 gatgatgaga atatgaaagt ctttttcgag agaaaaaacc tttcggataa tattttcaat
    50461 attctccttc tcgttgtagg ttggaatgat aactaatgaa tcggacacgg cattgatttg
    50521 cgcaaatgta aaaaaaaaac ctgattttcg ttaaaaaaaa attgacattt atgtaaataa
    50581 gttatatatt tacccccatt atggggttaa gatttctact attccccttt caatggggtt
    50641 acatattgtt aatttctctc agaaaactgc tgtatgccgt tgggattaaa cgacggcgca
    50701 cattcaatat gcccataatc tgcattggga atctgaatgc aggcggatcg ggcaaaacac
    50761 cgcatacgaa atattttgct caattactcc agcgcaacca ttacaaaatt gccattatac
    50821 tgagaggata caaacgcagc acgcgtggtt tcattcactg caacacagat cacactgtag
    50881 ctgaaatagg tgatgaagca tatgaatatg taagaaattt agacgaaaaa acaaacatct
    50941 tcgtttgtga gcgacgcaga aaaggcatta aggaaatcat gaataaattt cctgaaacag
    51001 atgtaatatt aatggacgat gggtttcagc atctggctgt taaagcaggg atgtatattt
    51061 tattgaccga ttacctgcag ccgtattgga aagatcatgt tttacccatt ggaaaactcc
    51121 gggaatgcaa ggcggcaaaa aagcgcgccc atgtcatcat ggttaccaaa acgccaaaag
    51181 tattttcacc gctggtcaag cgcgacctta ttgaaaaaat ccgtccacgc tatcatcaac
    51241 atgtcgcatt ttcgtatctt gattacggaa accctgtccc tgtttatgcc gaaggagatc
    51301 agcgcttcac cgcccccgaa aacatttatt ctgcctttct gctttgcggc attgccaatc
    51361 cttatcccct tgaagaattt ctaaaacgta aatgcatcga gctgtattcg cataaatacc
    51421 ctgatcatca cgcatataaa ttaaaagaaa ttgaaaagct cgccttcgat ttcaataatc
    51481 acatgatgaa aaataaagta atttttacaa cagaaaaaga tgtttcgaga ttaatgctgc
    51541 ctgagattaa agaaaaattg gtagatttgc cattctttta cattccggta actatcggaa
    51601 ttcatgaaag caaagaattt aatcttgaca aacttataat agactatgtt ggaaaaaatt
    51661 gcaaaaacag ctgacttcat ccgcagcaaa accaagctca gacctcagat tgcaatcgtt
    51721 ctcggaacag gattgggagg tttggttaat gaaatagaaa taatcgacag catctcgtat
    51781 agcgaaattc ccaatttccc ggtttcaacc gtgaaaggac atcaggggcg actgattttc
    51841 ggccgtctga gcggcaaaga cgttatagcc atgcagggtc gtttccattt ctacgagggt
    51901 tataccatgc aagaggttac atttcctttc cgggtcttta aaaatcttgg aattgaaacg
    51961 ctgctgttat caaacgctgc cggaggcatg aataaaaact tcaaagtcgg cgacatcatg
    52021 ttcatcaacg atcacatcaa tctgatggga accaatcccc tgattggaaa aaactacgat
    52081 gagctggggc cccgctttcc cgatttcagc gaaccatacg accacaaact tctggaaacc
    52141 gcattgggtg cagcaaaaga cctgagtatt ccggtgcatc agggcgtata tgtagccatg
    52201 agcggcccga ctttcgaaac ccccgccgaa taccgctatg tgcgcattat cggtggcgat
    52261 gccgttggaa tgtcaactgt tcccgaagcc attgttgcac gccattcttc gatgaaagta
    52321 tttgccgttt ccatcatcac agacctgggc ggcatggacg aagttgtcgc tgtcagccac
    52381 gaagaagtga ttgaagccgc caacaaggca gagccgaaaa tgacagccat catcaaagag
    52441 ctactgaaaa gaatttaagc ctaaaccctc gaagggttca aaacccttcg aggtttattg
    52501 gcacctacag caaaattttc aacaattctt tgagctcgct gacaacataa tcagccagct
    52561 caaacggacc ttcagccccg gcatgattca ccagaatggt tttcatccct gctgttttag
    52621 cacctacaat atctttttta taatcattac caatcagcac cgatttgtcg atgcagaaat
    52681 cagcttccat acaaatgcgc agaaaaaact caggatcggg cttacacacc cccagatctt
    52741 tggatgtata gtgatttttg aaataatgat cgatttttcc cattaccagc gctctgcgca
    52801 tatcggccgt atcagactca cccgcattgg atgccacaga caaaacatat tttgcggaca
    52861 gtttttcaag aacctcggcg gcatttggaa aaacttcaat acgctcccat tgatacatag
    52921 gtcccggcgt tccgggaaag tcacgcatca gtgtgtcgcc ccaatcaaat acaatgtgtt
    52981 caattgcttc cataaactgt tttattttat ccagacgccc ggaatgatac gtccgcattt
    53041 attgcattta ttctctgtca tgccgctcac cttagtgatg tatccttcgc gcttcacaag
    53101 cagggttccg caatcagggc attgcgtatt ttcatgtgct tcgttgcgaa tattaccggt
    53161 ataaacatat ttcattccag cgctggtggc catatgaaat gcttgctcaa taacagccgg
    53221 atttgtcgga tgagaatcca ataatttatg cgccggaaaa aatcttgaaa tatgcaatgg
    53281 aacttccgca aaatcatttt caatcagcca ggaagtcatt tgcgaaaatt catcagtttt
    53341 atcggtataa gcggtcacca tcagatttgt tatttcaagc catttccccg aatcccgaat
    53401 ggttttgatt gtatccaaaa ccggctgcag agagccattg cagagagtct tgtatgtaga
    53461 atcattaaaa gatttcagat caatattaaa ggcatccatt accccgatca gatcttttaa
    53521 tggctcctga ttgatgtacc cgttcgaaat cattaaagtt ttaattcccg ctttctgagc
    53581 cagaaaggca gtatcataca tgtattcaaa aaaaacggtg ggctcggtat aagtaaatgc
    53641 aataccatcg acactatgtt cacgcgctgt tttcaccacc ttttcaggtg aataatccag
    53701 tgcagaaaca tcattgggag ctgcctgcga aatatcggca ttctgacaat tgaggcatcg
    53761 gaaattacaa ccggccgtgc caaaggataa aacttttgaa gacggcagaa aatgatacaa
    53821 tggttttttt tcaatcggat cgatatgaac agcaaccgga ttgccgtagg ccatggtata
    53881 taaaacaccg cccacattaa ttctggtacg acagataccg gactgcccat ttttgagaat
    53941 acaggcatgc ggacacaacc ggcatttaac cgaaccatcg ggcagctctt cgaaataccg
    54001 ggcttttatc attccattat gtcttattga tttaaaatta ctcatggatt cacttgcata
    54061 aagacgcggc atagcaactg cagcaacacc cataacaaaa agattcttta tgaaatctct
    54121 tttctccatg acaatatctc tttcaacaaa agtaacaatt aacgtttgat aaaaaaaaat
    54181 tctatcttta caatatgatg aaacgcatgt taattttgct taccattatg agtatttttt
    54241 cagacacagg agcccagaaa aatctgccga tacgtaaagc agccgttgcc ggacaattct
    54301 atccggcaga tgccgaagcg ctggaaaaat attttgaaaa ggaattcaat cagattacta
    54361 tcgcgcccaa cgataaaaaa gtcagagcca tcattgttcc gcatgcagga tacatgtaca
    54421 gcggacgcac tgcggcctgg gcatttgcca cccttcgcga aaaaaacaca tataaaaacg
    54481 tgtttatcat cggttccagt catatcgcag ctttcgacgg tgcaagcgtt tttcatggcg
    54541 ctgcgtttga aactccgctg ggacaagccg aaataaatac tgaaataacc ggcactctcc
    54601 aacaaaacaa tattttcaaa tttccacaac aataccaaac cgaagatcat gtactcgagg
    54661 ttgaaatacc ttttttacag tacatcctcg aacccggatt taaaattatc cccatcacca
    54721 tcggaaccaa aagcacttat gcattggaca aaattaccga cgcactgaaa ccctatttta
    54781 cacccgataa tctctttgtt atcagcactg atttatcgca ctatccctca tattccgatg
    54841 cagttaaagc cgacagtgct gttgtacaat ctattctgag cggtaatccg caaacatttg
    54901 atgccgttgt gcgatcaact gaaaatgccg gaataaaaaa ttatgttact gcgatttgcg
    54961 gatacagcgc agtttacata ctgatgtctt tagctgacga caaagatgga ttttcttatg
    55021 aaaaactgaa atattcaaat tcaggcgatg tctcaggcga tttcgaccgg gtagtcggtt
    55081 atgcagctat agctgtcaga gagtgtgacc tgaaagaaac cctacaaaaa aaagatacat
    55141 ttactatttc tgaaaatgat aaaaaaatca tgttcaatat tgcacgaaac agtattgaat
    55201 cgaaattaaa cgataacgta cacacagtgt ccgacacact ttccgacatg ctcaataaaa
    55261 aatgcggtgt atttgtaacg ctgcatctga ataataaact gcgcggctgc atcggtacat
    55321 tccggcaaga tcgggaactc gccatcaata ttcatgaaat ggctttggca gcggcattca
    55381 gcgacccacg atttgatccg cttacaaaag aagaatataa aaatattgaa cttgaaattt
    55441 cggttctgac cccaatgcag aaaattgatt cagtgaatca aattgtttta gggaaacacg
    55501 gtatttacat caagcgcggc aacatgactg gaacttttct gccacaggtt gctaccgaaa
    55561 ccggatggac ccttgaagaa tttctgggac attgcagccg cgacaaagcc ggcatcggat
    55621 gggaaggatg gaaggatgaa gaaactgaaa tttttattta cgaagccatt attctgaacg
    55681 aataatgcat gttttggtct tgcagatcta ataatccccg catttataat tattgtcgct
    55741 tcagtcaacc atcatcatcg acgatgcgaa atccttaatt acaaaatgca catcggcatt
    55801 gtaactgcca tagaccggat cggttaattg ttctgaaacg agtttcaggt tcatttccat
    55861 attggctttt tcgtccttga aatcaaaaga gaggatcgat atttcatcaa acctgaaata
    55921 caaaaaagca gaaccttctg gtgatttgat tattgattgt aaatatgcac tggccttgat
    55981 ttccgaatca cgaagcgcat ccttatatgg aacataatta tcgcccttaa gtgaatacgg
    56041 gagttttgtt tcatacaatt caaaactacc atcatggttt gtatgccact tcagttgcgg
    56101 tgctgccggc aaaaacaaat cgagtgaatt tacccgtcct ccgtcatttt cctcaaattt
    56161 caaaatcaaa gacgtgctgc tccctgagct ggttgtaaca tctgtcagcg gaatccggag
    56221 tgtgtctttc aaaggaaagt ttttttctcc ttccgagttt tgatgaagca taattgttgc
    56281 ctcattaaca acaacttttt caatggattt ttcattatcc ttttttgttg ttgatgaaca
    56341 tccaatgaaa aaaaacatta aaaaaacaag tgaaaaaaaa gtttggttca tattgagtta
    56401 ttttatcctc gttcctttaa tatccatttc cgtacgattt tcctttcctc taagatgatt
    56461 gatgcctaca tatttgccct caaaggaatt gcggccggta aatctgattt tgtactgtac
    56521 aacaccaaga gcttcaggat catcaaaacg cttggattgt ataacataat actgtccttc
    56581 ttttttcaca tccatcatgt attcaatgcc atccagtctc agatcgggca actgagcttt
    56641 ctttcccttc atttcttgtt caactttttc acgttcctta taatactcat cgcccgaacc
    56701 atacatgtcg gccatattat caatatactg caacgaagca tccatggtca ttttggtaac
    56761 agtagcatcg agtttccagg tgccttcaag ttttggagac ccaatcataa tttcaaccac
    56821 cgtggttttc ccgtttcctt caagattcaa ttcgccggta aattcttcat cttttccagc
    56881 gggaccagcc accgtaacaa tcacctcctg cgggctgcct gcatcaagaa aaccgagcgt
    56941 tgtacaattg aacaaaaatt cgccatcctt atcggtagtg cctgtccata gttttttatg
    57001 aacatcggat gcaacattaa attgcacagc caatccgctg acatcaacat cagcgtattc
    57061 ttcacattcc ggatcaatct ttacaataac acggattttg ttcactttat ttaattcctt
    57121 ctgtatataa cacaatttat catacagttt accctgcata tcaagacgga tttcctttac
    57181 aatgcgttcg aaaatattcg tttcattcaa cgttttgatg atggaagcaa aatgctcatc
    57241 cgaaatatcc tttttcagct gctcattcag ataaccccca ccagtagcgc ctgtattctt
    57301 catcacgcga tcctgatact ctcccacaac gctttcatct ttccagaatt cccagacata
    57361 atcatgaata acctttataa cttcttcgga tgctgtagca ggattcttaa ccgacttcat
    57421 cacctttttg agtttcttgt accaatacac ggaattgatt ttctcctttt ctctcttttc
    57481 tttatagtac aagtcatatg ctttctgata aatattgacc ctgccactga tggcttcctg
    57541 aatgaattta ttcaaagacc aatcaataac aaaaacgcca acaaaagcaa gattgatggc
    57601 cgaagtatta atcaatttca aagcccagta gttgtacagg ttttttgata attccagcgt
    57661 tgccttacca gtctttccat ctgcaacatc aatggcacat tgcaccaaag caaagcccaa
    57721 cccaacttcg gtagcgactt cgttaatacc tttcaaagcc cccatttcaa gcgcgttttc
    57781 agcaaaactt cccacagctg cgctgattcc aaaccattca ttcaccttat cccaaccagc
    57841 tctgacagcc ccatcggact ttggtgcagc agcagcaaat tcctccatgg ttgaaacaac
    57901 ctcgtcggat gacataaacg caccacccgg aaactgcact ttttgtactg tcatcatcgg
    57961 acccggagca actttttcag atacagccca gggagcaaac gtagtgaaat gattggtgta
    58021 aatagtcatt gtgttatcag ccgcatttac tgtataggga agtcccttcc actcattgct
    58081 ggcttcatcg agatacatgc aaaaaagatc tttcgacgga tcagcacctg ccggcagatc
    58141 cgcaggattg tatttaatgg tgacttccat gtacccatca aattcattcg tctccgacat
    58201 acttacatcg taggccccaa gctgttttat gccttcttct tcgggcattc ctttcactga
    58261 ataaatactc aatgtttgct ctttatcaat caatccaaat ggcacagtca cggcaatctc
    58321 atcctcgtac ttcacagtct gatctttatc agaaacttca attttctgct ctaccagcag
    58381 tttttttcta acccctccga aaaattcatt ataggcccaa taacccccac cacccaatac
    58441 agcaacgatc agaaccgctg ctgcgacttt cagaaaaata cgtttcttct tttgtggcgc
    58501 aacaggtgtg gactgaggtg cgggaaactc aacggccttt ttgggctgca cacgaactga
    58561 aaccggctcg tgaaccggcg ctgcagcaat caccgctttg ccacacactg tgcagaattt
    58621 ggcacccggc ttcaactctg caccacacga acaaaggttt tctttgattt cggccggctt
    58681 gtccgcaaca tcctcaactt ttttgccgca gacagtacaa aacttcgagc ccggcttcaa
    58741 ctctgcgcca caggaacaac ggttttcatg aatgacttca ctgtttttcg ctgacaattc
    58801 aattttcttt ccacaaatag tgcaaaactt cgcgcctggt tttaaatccg ctccacaagc
    58861 gcatttcgtt aaaacatact tcttgggtaa tttttctgca ataacaattt tacttccaca
    58921 atttgtacag aatttggcgc tgtcattcag ctgtgcatgg cagttcggac attgtttcat
    58981 attgaattga tgttgttgtt ttacttttct ttccaaatat acaaataaac aaacatatag
    59041 aaacattgca aattttacac aatcagcaga attaatttta gtaatattgt atcaccaaat
    59101 tacccttatg atccgaaaca aatacagaat aattgcaaac cgaagtgctg tcttttttgg
    59161 ggctattgca cttctgctct ttctgcttcc atcgattacc ggcacagatg ggatgaatgg
    59221 tggctttgcc ttgattgttt ttgccattgt ttttttgata acaggtctca ttacatttta
    59281 tttctacaac agaaatgcac gcgtttttga ttcgctgctg caacctcaga aaatccttgc
    59341 tcattgggtt gtgaaacaaa acgaatatgc acaattgcag caactcaacg ccaaagaaat
    59401 atcaggtaat tttatgctga tgcgatgggt agtcattatc attactttta tcgtttgtgt
    59461 ttttctcgcc attgctggac ttgaattcag ttttctttta ttgttttttg caggactcag
    59521 cgcttttatc tggattgtaa gtgccattgc cattcattct caaaaaaggc atctccttcc
    59581 aaccgatgcc caaatcattc ttacaaacga gggcggattc atcaacgaca cgatgcatgt
    59641 ctgggcaaaa ctcaacaacc gccttgaaga tgcagccata aaaaaaataa aatccggcat
    59701 acactatctt gaaataaact attccgtacc aagcagaggt gcacgcgttg aaaccaccgt
    59761 ccgcttcccc atccctgatg gcaagctgac tgaggcgcag tatattctgg gcgaaattta
    59821 caacccccaa taattacaaa tatgaaaaac aacaacataa aaacactcat tgaagctgca
    59881 tgggataacc gcgagctgct caacaatacc gaaacccgcg atgccatccg cgctgtgatc
    59941 gatctgctcg ataaaggcga aattcgtgtt gcagagccaa agggagacaa gtggcaggtg
    60001 aacgaatgga tcaaaaaagc cgtcatatta tatttcccga tttcggaaat gattgtaagc
    60061 gaagccggcc cacttgaatt ttatgataaa attccgctga aaaaaggtta taaaaaagcc
    60121 ggaatacgcg tggtgcctca cgctgtagcc cgccatggag catacattgc aagcggtgtc
    60181 atcatgatgc cgtcgtatgt gaatattggc gcttatgtcg atagcggcac catggtcgat
    60241 acatgggcta cggttggctc ctgtgcgcag atcgggaaaa atgtccatct cagcggtggc
    60301 gtcggtattg gtggtgtgct tgaacccgta caggccgcac cggtcattgt cgaagacgga
    60361 tgctttatcg gctcacgctg cattgtggtc gaaggcgccc acatcggtca ggaagccgtt
    60421 ctcggagcca ataccgttat taccggcagc acaaaaatta ttgatgtctc cactgaaaag
    60481 accattgaat acaagggctc tataccaccc cgctctgtgg tgattccggg atcttacacc
    60541 aaaaaatttc cgggtggcga attcaatgtc aactgtgcac tcatcatcgg acagcgcaaa
    60601 gaaagcaccg accgcaaaac ctcgctcaac gacgcgctgc ggaatttcaa cgtggatgtg
    60661 taactcgccg aatttgacta tttttgtaca tcaaagaatt tttctgcatg acaaagacat
    60721 tctctaaaat tgcatccatt ctggttttca tctcaatttc cctctgttca tggggacaaa
    60781 gaacaaccat cctcaataat gaattctctt atacatcaac atatcaatac gtatcaaccc
    60841 ctggcgccag agaattttac agcaatttca tcattcagga aattgctcgt tccattccca
    60901 aaagagcaga atatacttcg tttacaatta attatataat gaatcaacaa ctgataaaac
    60961 tcgattcagc tcactatatt ttcaaaccgg aactgaaaaa tttcgcaacc accggcgatg
    61021 ttttatacaa aggaataaac atctccaaaa aactgattcc gggcgagata gactataccg
    61081 taaaaatcta tcgcaaacaa agcggtacga tgaacatcgg cggtcaacaa aatggcattc
    61141 ttgtttttca aaaaaatgtt accggtcagc aactcaatga taaccttggt tattttccgc
    61201 tccctgagct cgctttcagt gataccgtca atgacgctca atacacactt gccgtcgaaa
    61261 atcaacatct ttatttcaac gaaaaatcaa aaaatatttt tttgaatcag gttgcctaca
    61321 tcaacgacta ctatgcaagc gacgcactga taggagccca gatggacaaa ctgcacgcca
    61381 tacagcccga caatattgat ctgatttctt tttacgatat cgaattgaaa gaagttgaac
    61441 aaagcatcgc ttcgctcgac tcaaggcaat ttccgcaaaa tcttggctta cacgaaaacg
    61501 atccgatgca gtttatggcc aaaatgcaca ctttggtaac agaaacatca cagatgcgag
    61561 ccacgctcaa tcaacggctg tcggtactcg atcagctttt ttgtcagaaa ggaaatgatt
    61621 tgctggctga agggaaatta caagacgcag caacatatta caacaaagcc atccagatca
    61681 atccctatta tgcacccgca caatatcagc tggcacgcat ggaatttaat gctgcaaact
    61741 atgatacttc ggcatacatg gtcaatcatg ccttgctgaa aatgagcccg gatccttcaa
    61801 cacagcaact gcttgttcaa cttggcaaca atatctacac aacgctgctt ggtaatggcg
    61861 acaaatataa ttcggaacaa aaattcaacg aggccatttc tgttttcgat cgagcgaaat
    61921 ggctctgtac cacaactcca ggacttgtgt gtaccgaaca ggttcaaaaa ggactggctc
    61981 aggccaaata cggcatttac cgcagctatc ttcaggtggc cgaaaaagcc atacaaaacg
    62041 gacggctcga tattgccgga aattacatta atcagagcat ggaatatcag aagcaaaatg
    62101 ccactgaaat tatcagcaac gctgaaagtg ttcaatggat gggcattctg tgcaaagaat
    62161 atgtcatgct gggaaacaaa ctcaatcagc agaaaaaata cgatcaggcc attattgaat
    62221 ttaataaatg cgacagcatc agtcaggtct atccttccat cggcgaagtg aaaggttgcc
    62281 agacaggact ctgtggcgca cgcaatggca tttacaataa ctatctcgaa gatgcccggg
    62341 atcggcttgg caaagatcag ctcgatgctg ctgaagagaa atgtttaacc gccatggctt
    62401 atcagaaaca atacaaagac gacatttttt cggatcgtga tgcaacagaa gtaatggctc
    62461 tgataaaaga aaaacgctac catattgcca ttgataacgg taagaattat ctgaacggaa
    62521 acaatcattc tgaggccgct aaattttttc tcgaagcaaa cggatatcag cagaactaca
    62581 atttccggcg caacgacagt ctcggtattt atatccgcac cgccggaaaa ccgctggtgg
    62641 ctgccattgt agaagaaggc aaagtacagg catggggcaa taaacttgcc gaagcccgca
    62701 attcgtatca aaaagcaaac gatgatatca aactctatgg cctcgaaaaa gacaaggaac
    62761 tcgaagcatc gatgaacgaa ctgaaaaata aaatattctc gcaggaatgt gccaatgccg
    62821 aacaaagcta tctctcattc cttgataaat cgaaaaaact catgcgcgac ggcgactatg
    62881 ccctcgccta ccttacactt tcgcaagcca ttgatattac cagcaaaaat gttctctgca
    62941 acatcgacaa cagtacggca tacaaagagc agaaccgcat caaaccggcc atggaatatc
    63001 agaacctgat gttcaaggtg gatgaatatt cggctcaggg caattacacg gagtgtctga
    63061 aacaaataaa caactgcgga attttttaca ccaccaatgg cattgattca ttcggactga
    63121 agcacccgac accgcaggaa tacattctgc gcggcggcga tgtgaatttt gtctatttcg
    63181 gtgctgatca tttctatggt ctgaaagatt atgaatcggc attcaaaatg ttgcaggaac
    63241 tgaaaagccg caattatccg gttaaattca caaaaacaat tcaaacgcaa ctcggcggta
    63301 aaatggccat cagagacaaa ataaacgggg taaccaatta taagatccag attctgaaat
    63361 acaccgaagg cgaaaaatgg tatgccgttt tcagcaaagc gtataagaag agctggaaga
    63421 aaaattgatg tgatgatgtg ctgatttgaa aat
//
LOCUS       DAOPKE010000005        62799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_479_length_62799_cov_13.223750, whole genome shotgun sequence.
ACCESSION   DAOPKE010000005 DAOPKE010000000
VERSION     DAOPKE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62799
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_62799_cov_13.223750"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..1086
                     /gene="nadD"
                     /locus_tag="PKY63_01190"
     CDS             490..1086
                     /gene="nadD"
                     /locus_tag="PKY63_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPE99255.1"
                     /translation="MTSKKQIGLFFGSFNPPHIGHMAIANYLLAFGNIDELWFVVSPQ
                     NPLKEKKSLIQGRTRLEMVRRAIGNYPGMRVSDIEFGLPTPNYTINTLVHLEEKFPDY
                     AFWLIMGMDNIQTFHKWKSWETILEQNKILVYPRPGFDGNPHVKHPNVVLINAPLMEI
                     SSSFIRKAIGDRKNMRYFLPETIFEMIEKEGLYKKQEI"
     gene            1282..1369
                     /locus_tag="PKY63_01195"
     tRNA            1282..1369
                     /locus_tag="PKY63_01195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1316..1318,aa:Ser,seq:gga)
     gene            1429..2250
                     /locus_tag="PKY63_01200"
     CDS             1429..2250
                     /locus_tag="PKY63_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPE99256.1"
                     /translation="MKKLISLVAIMGVLFFSTTQVSFAQEANTDTPTTEVVDSTASNT
                     TGTQVTPAGGEEVVADKSFHQILKEKFIEGGATWMTPILICLILGLALVIERIVYLNM
                     SRTNTEKLLQKVEESLQANDIEGAKEICRNTRGPVASVFYQGLDRFNEGIESVEKAIV
                     AYGGVQMGRLESNLSWIGLFIALAPMLGFLGTVIGMVQAFDDIEAAGDISPTVVAGGM
                     KVALITTVFGLIVAIILQIFYNFVLSLIEKLVNDMEDTSISFMDIVFRANNTQKQ"
     gene            2255..2650
                     /locus_tag="PKY63_01205"
     CDS             2255..2650
                     /locus_tag="PKY63_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99257.1"
                     /translation="MKSVKSIAIIAILILLAAITLFAGIDLRLYVTYAIFAIAILALV
                     AFTAYSAATSGKKSMTTIILGAALVGIVLLFYFITPTDDVPVALFEKTGTSMGWSAII
                     GAGLFSIYALLGIFVALLVFFGVKNIVNK"
     gene            2661..3233
                     /locus_tag="PKY63_01210"
     CDS             2661..3233
                     /locus_tag="PKY63_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPE99258.1"
                     /translation="MARKVPELNTGSMADISFIILIFFLLVSNIDTDLGLARKLPPMP
                     DPDAEVPEVNERNVFVVLVNAYDMLLVEGKPGNINTLRKEAKKFFLNPTNDKNLSGKE
                     MVNIPFIGPYEKSKGVISLQNDRGTSYEMYVKVQNELAAAINEMRDDLAKSKFGKPFS
                     ELSEDYSKAVSKAIPVAISEAEPKDIGGKK"
     gene            3233..3700
                     /locus_tag="PKY63_01215"
     CDS             3233..3700
                     /locus_tag="PKY63_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPE99259.1"
                     /translation="MARFKKSGNKSVPGINTGSTSDIVFMFLFFFMVITTIRQATLFV
                     RVSAPNATEVQKLERKNLVSYIYIGNPINAKTYGSEPRIQLNDQFATSDDVAEFIEKE
                     RAARNEADRQFLTTSLKVDRKVKMGVVTDVKQELRKVGAFKINYSTKKGKVTD"
     gene            3832..6795
                     /locus_tag="PKY63_01220"
     CDS             3832..6795
                     /locus_tag="PKY63_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99260.1"
                     /translation="MKSISLQEHINQSRIAAAANRAKGNRHGQILSMLYSDPYHFVEE
                     LIQNAEDAIARRKNESVSGFVKIIISETGISFFHNGDPFSETDLMAITTFASTTKKGL
                     PGINQIGKFGIGFRSVYGITDNPEIHSGDFHYRISDFEVLEECAPRHAEAFTTFIYLP
                     FKPSVSADFRKLLQNRICALNPTFLLFLKQIQQIEIIAENQSVVINAATELCEKKITR
                     KTIVSSNNGIRNELHFLLMQRNSGHNHKAAIALQINESGHFIPIRNPQVSVYFPTQFH
                     ITHSIIVHGSFTTTPNRENIPFSEEWTPENYAVLSVISDLMRILLRSLLNKKIITADF
                     WSLFSWNSSSEDPVSAVISECLNKFIAQEKCLPDQSGKLQSVSGLCVSEDDNLIQLIT
                     AKDIMNVYGRSGFLHYDITDNEIFVSFLRKSHKLKVADIDSFAFHIASQKEFLQKKTL
                     TYFPSLYRFLALHPRLWDHTHKGRYYNLRHKAIIPDKGKHLTAPFSDNDKPQIFIGKA
                     ASGFSVVHPELSSNADCMNFFSMLGIPDFAPGLSEANNLFRRFKEKTASVWWHNLYEI
                     YASSEASVKDHIREKTNELNCLHCIDSQSGNKIFVQPQQAYLADEHLTAFLSGQQSFF
                     VNPQLIAFMSDQNIAASSFGHFLTELGVNAALRIVETESQLDDNHKSALRAGYEQFSI
                     VHETITDWTIEGLDSFLISPKPASAAALWHLLGKVDEKHRKACYTFESYVRSETIQFS
                     PTYIEKLKTNAWLFDEHLNAVSPQGFQMERLHESFNQSKPASLWMAAELGMITENISS
                     EEKEILSLIRTHRLNTELLQQLLDDTKNDVEFFQYLPMSEPASIAKNTMNFSTADLSA
                     IIQSNPFNANNSQSWDQYLFEEKTQILKNHFLNTVLSASKQSKPYIAKPGHLQIKQNE
                     IIKAHYFTGIRPDAGEEILLPSSFANHFKNESEKKDTGLFVFNLHDKTCIEVSPDNLA
                     EFLNSHSIAFIKTTK"
     gene            6802..8127
                     /gene="tilS"
                     /locus_tag="PKY63_01225"
     CDS             6802..8127
                     /gene="tilS"
                     /locus_tag="PKY63_01225"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPE99261.1"
                     /translation="MTELDSFTKNLSTLCPDFRQKKYLLGISGGIDSMVMLHLFQRAG
                     LDCSIAHVNFQLRDSESDRDRNFVFEEAAKAGIHILEKTCNTKHFAQKNRMNIQDAAR
                     KIRYDFFTETADINNFDFICTAHNLDDVVETLIMGLNRRGSLNTLSGIREMENRILRP
                     LLHFSRSDIESYAAGNMVRYVEDSSNSSDKYLRNKIRHHLLPLFEEIMPGFSKRAAGS
                     VSFLREYQKFVASAAAAELLKLGNAQKEGIDLLQLQNHPSGKLLLMQFLLSHGFFPAD
                     AAEIFRIDNYSEPKEFLSNDKKLIVHRGKIILSEIKTFEDNQIWYIDEDMNTDHLPVL
                     LQMEYCQINRKEELICKKDTALINPVEINFPLLVRKWIPGDRFIPFGMTQFKKVGDFF
                     TDAKTTVAERNSTYVLCSGPHIIWIIGYRTDQRFMINDIPATALRIKTR"
     gene            8170..10254
                     /locus_tag="PKY63_01230"
     CDS             8170..10254
                     /locus_tag="PKY63_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPE99262.1"
                     /translation="MILRRFPLFFAMLLLFSAVQSQIIKPVKWNSRVDKVSDTEANIV
                     LTASIDQGWHLYSQYNPEGGSLPLVFTISPSNAFALVGKVAEYPKPTEHYEEVFKITE
                     KFWAGKAYFTQKIKVLTDKPFDFEISLSGQACQDDGYCVQIEDDLVVKVDGSQYNKEN
                     PVDTTVIAVVDTGKIKPDTSKTETATLSPDIEDAEKAGSGIWGVIIEAILWGFVALLT
                     PCVFPMVPMTVSFFLKGSENKAKSRFNASVFGISIVALYTIPIAILIGVTYFVGGQSI
                     TTDIFNFLSTHWIPNVIFFLVFIIFAASFFGAFEIVLPSWMTSKADSKADRGGILGSF
                     FMALTLVLVSFSCTGPIVGTIIVKSTQGEIWEPIITMLAFSAAFALPFTLLAFFPSWM
                     KNLPKSGGWLNSVKVVLGFIEVALGFKFLSVADQVYHWGILDRETYLAIWIVTFTLLG
                     FYLLGKLKFKHDSEVKYLSVGRLGMAIAVFAFVVYMIPGMWGAPLRVLSGYLPPQQTL
                     DFDMPRIVREAAMLNGGGSGEPTKLCEKPKFEDILPSLPHGLEGYHDFDQALKCAKEQ
                     NKPLFVDFTGHGCVNCREMEANVWSAPEVLKRLKNDYIVVALYVDDKTELPESEWITS
                     TYDGKVKKTIGRKFADLQVTRYEQNAQPYYCLLDTAGKDLVKPRAYDLDVDAFVQFLD
                     NGLKEFKARQGK"
     gene            10661..10993
                     /locus_tag="PKY63_01235"
     CDS             10661..10993
                     /locus_tag="PKY63_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99263.1"
                     /translation="MNITVEIIIAGMLALFVLFETILINIFKKDKNALLTGMVSLLSV
                     LFGLYLCMKNGLILDYGTEQETIKSHAFTMYVLGVANFCIGLLFIGISFIKYIIWQTR
                     KVKNKSRK"
     gene            11125..11361
                     /locus_tag="PKY63_01240"
     CDS             11125..11361
                     /locus_tag="PKY63_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99264.1"
                     /translation="MSTVELRHTIIEMLSKIDDTSFLKAIKTIVESKANEDVYKLSDF
                     QKKRIKAGREQVKLGQTISNDALQKEIKQWLSTK"
     gene            11343..11657
                     /locus_tag="PKY63_01245"
     CDS             11343..11657
                     /locus_tag="PKY63_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE99265.1"
                     /translation="MAKYKIVWSIEARLDLLDILGFYIERNKSVSYSTKLNDKINKSL
                     NQIDQNPLIGIKTEIESVRALITGHYQIIYEIQEQTIVVVMIWDCRRDPEDMVIDLRT
                     NQ"
     gene            complement(11698..12309)
                     /locus_tag="PKY63_01250"
     CDS             complement(11698..12309)
                     /locus_tag="PKY63_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPE99266.1"
                     /translation="MNEKKDIRTVAIFCGSSAGADKIYADGAEELIRVLHKHNVSIIT
                     GAGCTGLMGIVAEEALRLKMHITGVVPSFFMSENVVHKGLSELIVTDSMATRKTTILE
                     KADACIALPGGFGTFDELFEVATAVQLGLQMKPIGLLNTKNYYNPLLEMLQNAVREKF
                     VREDHLKAIVSDVSPEALFDKMLHIDYQAPANWIEKLIERNSF"
     gene            12394..12469
                     /locus_tag="PKY63_01255"
     tRNA            12394..12469
                     /locus_tag="PKY63_01255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12427..12429,aa:Lys,seq:ctt)
     gene            complement(12479..12622)
                     /locus_tag="PKY63_01260"
     CDS             complement(12479..12622)
                     /locus_tag="PKY63_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99267.1"
                     /translation="MHGISLMRAIFFVAIDSKYYYTSSSCRLKSGAPQTFSAGPKKKA
                     TPE"
     gene            complement(12716..13336)
                     /locus_tag="PKY63_01265"
     CDS             complement(12716..13336)
                     /locus_tag="PKY63_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPE99268.1"
                     /translation="MRGEKVICLSCLSKIAKTDFHTYADNPVAQLFFGKLPIENATAM
                     CFYDKGGLIQHMMHQLKYKNRPEVGEYFGRRLGKILSEAALYANIDAVIPIPLHPKKL
                     KIRGYNQSKVIADGFIQGYPTEIIEDVLVRVEFTETQTRKNRWDRYQNVKDMFAVRNP
                     DKIKGKHVLLVDDVITTGATIEACAKHLLAVEGVRVSIAGIASPAK"
     gene            13476..14381
                     /locus_tag="PKY63_01270"
     CDS             13476..14381
                     /locus_tag="PKY63_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99269.1"
                     /translation="MENNDSQQKKNRTYKIIIGLQLIIIAILAVLLFTTKSSVDTVVL
                     EKEKSVQMSEELQVELDSLMAEHDKIKKEYGYLSDQLTTKDSTILAQAKEIETLINSQ
                     ADYRRIKKKLDALRQITQGYVNQIDSLYRVNQSLTDENTKIKGDLDNANKKADELIKE
                     KDDLSTKINDAAYLKGYNINATGYNIRSGSKESVTDRARKVELIRVCLTIGENPLVSP
                     GQKDVYVRIARPDNIILTQGSYSFIYKGQRIQYSAKTVINYSQKASTICIDYPRENIE
                     LPVGKYHIAVFADDHELGQCSITLR"
     gene            complement(14793..15284)
                     /locus_tag="PKY63_01275"
     CDS             complement(14793..15284)
                     /locus_tag="PKY63_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPE99270.1"
                     /translation="MESNIPGFLMNQIIPKLEKYCAFQERCISEVKLKLSRMNVSREY
                     WDSLLNYLIQNGFVNEERFAELFVRSKIRQKGWGPVKIKMELRKKSIPDLLIDRYISA
                     FQVSDQEELLMGWLQKKLKTIEKDEPLKLREKLIRFGISKGFETGKVFQAVNKLIKAQ
                     PED"
     gene            complement(15294..16841)
                     /locus_tag="PKY63_01280"
     CDS             complement(15294..16841)
                     /locus_tag="PKY63_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPE99271.1"
                     /translation="MNKELIIDAASGEVQIALIENKRLVELHAERGTHDFGVGDFYLG
                     KIKKIISGLNAAFVDVGYEKDAFLHYLDLGPQIKTLIKYQKEVISGKQVSVETFPLEQ
                     DIDKTGKITNVISSGASILVQVAKEPISSKGPRITTELSMPGRYLVLVPFSKRISISH
                     KIKSLNEKSRLKKIASSLQPVNFGIIVRTVAENRSFDELKADMDELIGRWNSMLQSLR
                     SAKPPVKILGELDRSFTIMRDLLNASFNSIVVNDNTMYSELRNYLSSIAPDQIDIVKL
                     HKGKTSVFEATGVDKQIKSLFGQKVTLKSGAYLIIERTEAFHVIDVNSGYKADSGKDQ
                     EQNALAANLEMAEEVARQLRLRDLGGIIVVDFIDMHQGANRRKLYEKLVEEMSHDRAK
                     HTILPPSKFGLVQITRQRVRPETHVEVLEKCPVCNGTGQIKAHSLIIDEIENTLAFLA
                     NEQNEKVLVLYTSPFIYAYLTKGFFKNIAKTWKKKLRCKLKIEPASNYHVLEYHVFNA
                     RGEEITI"
     gene            complement(16998..17330)
                     /locus_tag="PKY63_01285"
     CDS             complement(16998..17330)
                     /locus_tag="PKY63_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE99272.1"
                     /translation="MHPQTPTKALILNNIIMTKADIVAEISNRTGIEKITAQSAVETF
                     MDIVKNAMENGDNVYLRGFGSFIIKHRKQKTGRNISKKVSIIIPAHNIPAFKPAKTFV
                     ESVKRGKK"
     gene            17391..18461
                     /gene="mutY"
                     /locus_tag="PKY63_01290"
     CDS             17391..18461
                     /gene="mutY"
                     /locus_tag="PKY63_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPE99273.1"
                     /translation="MNPRPDISLFKHLINWYRQNKRPLPWRKYADPYHIYISEIVFQQ
                     TRIDQGISYFNNIIGKYPDFISLSKAHEKDFLNVWQGLGYYSRAHNILKAASIIAEKY
                     DGELPSEYDKLLSLPGIGPYTAAAISSIAFGLPIPVVDGNVKRVISRIRCIGIPTDKI
                     ELTNEIQDFLNQHIHHFDPSDFNQSLMELGALVCTPANPSCPDCPVEKFCGALKSNSV
                     EKYPVKAAKKTKPAVSINFYIIFCNDKKDAFYMVRRDSEKIWKGLYEFPCIETDESEA
                     SVNSTEQQFPAGFPLDHAQPVFRTNHVLTHRLIRASFFETVYKKKVPAHWIKVPVSEA
                     HTMPVHRLIQEYLEQMHDRKKQ"
     gene            18495..18938
                     /gene="ssb"
                     /locus_tag="PKY63_01295"
     CDS             18495..18938
                     /gene="ssb"
                     /locus_tag="PKY63_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE99274.1"
                     /translation="MKSINKVILVGHVGKDPLVTVFEGGRKKASFSLATTERYRDKTG
                     ADQSVTEWHNIVVWGPQAEVVEKYVRSGNPLYIEGRIKYREYTDKDGVKKRITEIYLE
                     NFSMLTSRSASDSGTEITGPDHTVQPSSNDPLSTPLDNANDDLPF"
     gene            18980..20257
                     /locus_tag="PKY63_01300"
     CDS             18980..20257
                     /locus_tag="PKY63_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPE99275.1"
                     /translation="MDPILLLIIASLVILSLFAFLRIAESAFIRISSEFLDEIESPTP
                     SDHRTDDFLDKPAPVLVAFSIMKYSLLILLTIAVFIATEICLPHTSFFVRSLCVLAVI
                     FVLIWIFGEIIPGFFNSCNERKTFQSCFFILIPATAIVSMLKGVLGFSFARHESRIAR
                     KDMISISDISDAIENSEVEDEEIMEKQLIQGIVKFGDLEVKEIMRARIDVASIRFDTT
                     FTDVMDKMLEAGYSRYPAYGNSLDDIKGIIYIKDLLPAFRTGEKDYNWQQHIRQAMFV
                     PENMKISQLLVDFQTHKMHMAVVVDEYGGTSGIASLEDVLEEIVGEINDEHDADSDQI
                     LARQIAENTFVFDAKIPIHDLTRIAGIDSDLFDKFEGEVETLGGIILSICGNFPSKNQ
                     NITYKNIEFTVLSMSKHRIGNVKVKINESNQES"
     gene            20235..20834
                     /locus_tag="PKY63_01305"
     CDS             20235..20834
                     /locus_tag="PKY63_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99276.1"
                     /translation="MNPIRNLSIVLFVAFAAAIVSCGGGNETYYPKPRGFYRLDLPDK
                     KYTNFDSVYPFSCRIPQYAHVESIESNEPGSVWFNLIFPAYNGSVNFSYKTINNNLYK
                     LTEDARDFANKHIAKANEIKEIRVSHAENHVYGLIYDIEGTNSASPYQFYLTDSTKHY
                     LRGAVYFNHIPNNDSIAPIIQRVKEDIDTLIGSLKWKNQ"
     gene            complement(20887..21402)
                     /locus_tag="PKY63_01310"
     CDS             complement(20887..21402)
                     /locus_tag="PKY63_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPE99277.1"
                     /translation="MKNVFVLFISLLLFVNVQSLQAQVKLKLGHINSTDLMEVMPGRD
                     SAQKVLQEFAKSLDEQLTLMNNEFQTKYQDYMTNEATFLEPIKQMKQKELLDLQTRID
                     DFKAQAQDLLSQKESELVQPLIDKAKKAIDEVAVEKGYNYIFDTGTGALIYYQDSDDI
                     MPFVKAKLGIQ"
     gene            complement(21433..21969)
                     /locus_tag="PKY63_01315"
     CDS             complement(21433..21969)
                     /locus_tag="PKY63_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPE99278.1"
                     /translation="MKTRIFIFAALLLASMFVSAQKFAYVDTQYILENIPDYQTAQNT
                     LDELSMEWQKELEVKFAEIDKLYKSYQSESFLLSEDMKTKRQNEIIAKEKEAKDLQKK
                     YFGTEGDLYKKRQELIKPIQEKVYNAIEELAEEGTFAVIFDRAGNTTLIYANEKYDKS
                     DEVLAKLGYKAGGLNTNE"
     gene            complement(22005..24521)
                     /gene="bamA"
                     /locus_tag="PKY63_01320"
     CDS             complement(22005..24521)
                     /gene="bamA"
                     /locus_tag="PKY63_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPE99279.1"
                     /translation="MFKSLWCCLILVLLHVAVSAQTDKIIGVDYRNPRPYEIAGITVS
                     GEFFASPDNVISVSGLRIGQSIKVPGDEISNAVEKVYSNGMFEQVIISVTAVQGTFIY
                     LDIYLQDRPRLSKYSISGISKSEADNVREKLKLVRGDVVSQHTLMNAKRLIRSHFVDK
                     GFFNAQVEVAQKPDTTAGNMVTLDFIIHRGKKMKIAEIAVEGNTLVEEKVIRKSMKET
                     KQKKIYHLFKASKYIPSEFEKDKAAIIAKYKELGYRDISIVSDSVSVLNEKELSLRIK
                     IEEGLKYYFGDITWVGNSKYSDKQLNEILGVKRGDVYNQSLLESNLFYNLSGRDISSL
                     YMDDGYLFFSVTPVEKSVVGDTINLEIQIYEGKQATINKVTLVGNTRTNDHVILREIR
                     TKPGQLFNRSDIIRTQRELAQLRYFNNEKLAVNPKPNPADGTVDIEYVVEETSTDQFE
                     LSAGWGLGRLVGTLGVSFNNFSIRNIFNGDSYRPLPTGDGQKLSIRGQSNGTHYQSYN
                     MSFTEPWLGGKKPNALSFSLFHSVQTNGVSKADDGTYSTSGTLLERQVMNISGASIGL
                     GKRLKWPDDYFMEYLTLSYQYYNFDEYFQTYSFTSGNANNVNFGFALSRSSSYDPIFP
                     KSGSEISASVTFTPPYSYFNDRDYTTATDQEKFKWLEYHKWKFNATLYTRLFGDMVLM
                     SRTKFGFLGMWNKEVGLSPFERFYLGGDGLSGFALDGREIIGMRGYTNSVLTPRDGSN
                     QSIGGTIYSKYTMELRYPISTNPMATIYLLSFVEAGNTWLKFTEFDPFAVKRSAGLGV
                     RIYLPMFGLLGLDYGIPFDDVPWYPAWKGQFHFSINSSID"
     gene            complement(24524..25276)
                     /locus_tag="PKY63_01325"
     CDS             complement(24524..25276)
                     /locus_tag="PKY63_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPE99280.1"
                     /translation="MTKPLQQDIPQHIAIIMDGNGRWAKARGLDRTEGHRQGAETVKI
                     IVRAAAKAGVKYLTLYAFSTENWKRPKEEVDAIMDLLVFMLERETEELNRNGVRLMTI
                     GDISRLSEKAQESLRKSIEFTSKNEVLTLVIALNYGARNEILRATSNIAKDVANNIIS
                     PDQIDEKLFESYLYTAEMPDPDLLIRTSGEQRISNFLLWQLAYTELHFTPVTWPDFNE
                     EEFNKALVTYSSRERRYGMTGEQIITNQDKKD"
     gene            complement(25288..26163)
                     /locus_tag="PKY63_01330"
     CDS             complement(25288..26163)
                     /locus_tag="PKY63_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPE99281.1"
                     /translation="MRRVFFTFLIVIAGFTGFSQQHELGLMGGVSYYIGDLNPYGHFL
                     TSQPAGGIMYRYNMNPRMAVRLHVLFGSLQAADSVILYNEDRNLSFRSPLMEFGGQYE
                     LSYYKFKIGHDKDFFTPYLFVGASFFKFNPQGYYKGDWYNLQSLGTEGQGTTAYPDRS
                     PYNLAGFAIPFGLGFKVGIGSSTVLGIEWGLRKTFTDYIDDVSTTYADPTVLMAQNTE
                     VAAALADPAQSAETEKTGFQRGNSSTKDWYSFAGLTLTFKIKDKSDSCPGGSRRRDSF
                     KSKYLRIMKSESSIF"
     gene            complement(26279..27175)
                     /locus_tag="PKY63_01335"
     CDS             complement(26279..27175)
                     /locus_tag="PKY63_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99282.1"
                     /translation="MKITWPILFLLAFLIVDTASAQQWRRERKEYVFGLGASNFLGDL
                     GGANQIGTNGFRDLDWPATRPGTSMGYRYRTGKQHCVKGMMHFAYLNGDDKKTEELYR
                     HNRNLNFRTPIIEINGQFEWMLTRQREGHIYKLRGVQGWRNINIESYIFVGFGLMWFN
                     PQGNYNGDWIALKPLSTEGQGLVETRKPYHRVQPVIPIGFGFKYAISREWSIGLEYGI
                     RKTWTDYIDDCSTTYFNNDEIRDEKGDIAAFMADPSLGEVAGQTAPNMQRGDPTDHDT
                     YMFAEITFYYKIPRGGFSIPKF"
     gene            complement(27261..28142)
                     /locus_tag="PKY63_01340"
     CDS             complement(27261..28142)
                     /locus_tag="PKY63_01340"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPE99283.1"
                     /translation="MRICVFSRSYSPDFNDEIQAVLDVVLQTGGQAIFCGHLQAQISS
                     AFSLPPNTRFLTEGETVGGIADLVVSMGGDGTLLETVNLIRESEVPVMGVHFGRLGFL
                     SNIRKDEFRKALNEFIKGNYTLSARTVLEIDDCESLFGKKIFALNEVSVHKSSASSMI
                     SINTWINGSFMNSYWSDGIILATPTGSTAYSLSCGGPILVPEAENLILTPIAPHNLSV
                     RPLVISDKDVITFVPDGRTEDFILSIDSSQIVIGRGQKVTIRKAPFTLNFVRRPADSF
                     FGVIREKLMWGEDMRNK"
     gene            complement(28155..29528)
                     /locus_tag="PKY63_01345"
     CDS             complement(28155..29528)
                     /locus_tag="PKY63_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99284.1"
                     /translation="MNIRFFFLIFLISVVFSVSAADSLYFIKHISIAGNGVTKESVIL
                     REMPFGIGDTVLMSDTAELALRAEQNLVNTSLFHFASVVFSDSGNVCVGVTERWYIWP
                     MPFLNIEERNFNVWLRDMSFEKATYGLYVYHENFRGRKEYLKLLFKTGYNQIYGVSYS
                     KPYIDKNQNWGISAALGFEASHSLSYLVLNHEPKTLKLPDEYVYSGLFTSVATTRRAG
                     MYQLHKISLLFESTRYADSLFQLNPDYAPADLWRRLTLNYQYRYDFRDVKNYPLKGWY
                     VDAELNLGNIAGFSEKNDKKFYLKSTSRKYFNLAGPVYFATGATMYVSLAGGNNFATA
                     GFMGYGNDFVRGYQYRVIPVKHFFIQRNNLKYNILQQRIFKILFVRTSKFNRVPVSVF
                     VNAFFDQGWSDRGLFSGANNLSGDYLAAFGLGIDLVTYYDKVFRVEFTMNRFREKGIF
                     LHFTAPV"
     gene            complement(29533..30222)
                     /locus_tag="PKY63_01350"
     CDS             complement(29533..30222)
                     /locus_tag="PKY63_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPE99285.1"
                     /translation="MSHILFLMSLFAADITPSAIPSVKTSTAASEALSLMEEYKITHL
                     AIVNNEEYLGLVCEDDLLGLNDFSQPIGNIKLMIQGASVDENAHIFDILRTIYQHKLS
                     LVPVTGKNNKLSAVILSDSVLRVVAELLSVNNPGGIIIIELNVIDYSLTQISNIVESN
                     DALIIASFVQTIPNSTRMQVVLKVNNSDIGKILQTFTRYEYNIVASFGEDMIDDILQD
                     RYELLMRYLNM"
     gene            30274..30993
                     /locus_tag="PKY63_01355"
     CDS             30274..30993
                     /locus_tag="PKY63_01355"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPE99286.1"
                     /translation="MTRLSVNINKIATLRNARGGNAPDVLKAAVDCQRFGAQGITVHP
                     RPDERHIRYADVYALKPLVTTEFNIEGYPSDNFIKLVLDVVPNQVTLVPDPPEALTSN
                     AGWRVTENMAFLKKVIEDFHSKGIRVSIFIDTDTQNIAAAADTGTDRVELYTEPYATH
                     FPHDRQLAVAEFSNAARAAIKAGLGLNAGHDLNLDNLRYFAENVPGLHEVSIGHALIS
                     DALYFGLENTIQMYLRQLQPA"
     gene            30990..31787
                     /locus_tag="PKY63_01360"
     CDS             30990..31787
                     /locus_tag="PKY63_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPE99287.1"
                     /translation="MKLFFREYGQGQPLVILHGLLGMSDHWIPLAKQFAEQFHVVVPD
                     LRNHGQSPHAEKFSYKHMQADIEELLSDLQIKSCNLIGHSMGGKLAMHLALNHSEMVD
                     KLIVADISPIDYPIDEDNEMVQAINRMNLSSVNNREELRQMIQWATNDPMLQGLILKN
                     ISSKGLSGFSWKPDLENITKNLQEIYDFRCHEFQIRASESIDKASFNKETLFLKGDNS
                     QYISENHYPKIAALFPQNKITTIENAGHWLHAEKPAEFYEVCMNFLL"
     gene            31984..36033
                     /locus_tag="PKY63_01365"
     CDS             31984..36033
                     /locus_tag="PKY63_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99288.1"
                     /translation="MIKLLPALLILITMLFSNTDAYCSNKFINQPGSYSGKTQYLKTV
                     AAGCLPASASAELNIGGVRARINNGGDMWWDLADLAEYEVPKGSGRTSMFAGAFWISG
                     QDINNNIKVAAHRYRANGNDFWSGPLTIDGTASTEAAICQDYNFISTIYRQDVTDFVN
                     YYNNPAAYPGYNIPSYFYTYPAHGDPSLGHSFYLAPFYDNDDDGVYDPQSGDYPLYDI
                     NNRYCHQPDPSAEDMNGLTNGGVLFDQMLKGDQTLWWVFNDKGNTHTETQGTSIGIEV
                     RAQAFAFSTIDEVNNMTFYSFEVINRSTETLYETYLGFFTDPDNGYGNDDLIGCDVPR
                     GLGYCYNGDDYDESGNGAYGYGSQPAAVGVDILKGPYMDADGIDNGAYIQHVDSLGNL
                     WFENCNAAVNGSNFGNGIIDDECYGLTNFLNFRGSGAIPPYGTDPDVAAEYFNFMRSI
                     WKDNTHMIYGGNGHIISGGYGPECNFMFPGESDVCDYGTGGIPPNGPKNWTEETAGNQ
                     PYDRRIVQSSGPFTMHPGEVNYMTIGIPWARANSGGAAEALELLKCADDKCQALFDNC
                     FQLLDGPDAPDIMVQELDREVILYLGNSNFSNNYQDAYAEADPTIVAPSGYNYDSLYR
                     FEGYQVFQVIDPNVTAADIADPDKARLVAQCDVENGAATLVNYVYSQQVGTYVPQIEV
                     EGANEGIVHSFRIIEDMFASGQNTHLVNHKKYYYMVLAYGYNEYEKYSSDPAMQFPGT
                     ISSNGQKRPYLAGRKNISVYTAIPHAPAPESGGTLINSAYGAQPVITRIEGQGNGGNM
                     LELNAASRNKILEDGFSAELTYEAGFGPVTVKVIDPLNVREANYILKLDSSSAAWAGE
                     INSCKWVLEEYDMSNHFVAAYSSDHDISSPFEQIFTNLGIAVCFVDGKNPGDLTHESN
                     GLIYSDIIYSNPSIQWLGGLSDADGSSAFNWIRSGTQDSENNMYNDFIYSGQYMDPEE
                     YYEKIIGGSWAPYRLASYENDNPALKSIATSIQTTNKMSNLASVDIVLTPDKSKWSRC
                     PVLETCDDPLLAQGGAGKLTLRHHASVDKNGDTLTTEAIYDGNEQGMGWFPGYAINLE
                     TGERLNIAFGEDSWLGSENGRDMLFNPTSDLETTLGQPLFGGKHFVYVFGHLSESAND
                     CPAYDEGKWLYDMFSQETITALRYIFTSAMWCSIPLSIDGEQWLNNECHIRIRVSKAY
                     NKNYSTFGSAAPQNNNYPMYSFNTSTLMTVTNDQTTAMNALDMINVVPNPYYAVDDYE
                     ENIYDNKIKITNVPSKCTISIFNLSGTLVRKFKNDNPHVTDIDWDLRNSAGKLVSGGV
                     YIIHVYAPGIGERSIRWFGSMKTVVENEF"
     gene            complement(36379..36735)
                     /locus_tag="PKY63_01370"
     CDS             complement(36379..36735)
                     /locus_tag="PKY63_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPE99289.1"
                     /translation="MNIESFREYCLLKPGVTEEFPFDETTLVFKVGGKMFALTDIEGV
                     FIIALKCDPEYSIELQERYSAITPAYHMSKTHWIDVNEATSFDEKLLMQLIDGSYDLV
                     FKGLTKKVRDQIYNRV"
     gene            complement(36736..37989)
                     /locus_tag="PKY63_01375"
     CDS             complement(36736..37989)
                     /locus_tag="PKY63_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPE99290.1"
                     /translation="MKYGSVFLRLIFIYAVFAVFGMNNLCAQSNKKKQLQNQKANIEK
                     EISNTSKLIKETQKNSRKTTQQIKLIESQVRNYESLISKTGAEMALLEREIERKKLTV
                     AKLKRELNTLKSQYARMVYFAYLTRKPANRLVYLFSSRSVMMAWRRMNYFREYSAIRK
                     NQVKRISQTSSDIELSLSELEGMRNEKLSLKSDQEKQKNQLASQKKEKDKVVKDLKKQ
                     ESKLRANLKKKQREATEIQRKIQKMIEAEIEASKKKANTVKANNKTSNSKPNAYQMTP
                     EEARISNTFAGNKGKIPWPVAQGAITDRFGEHPHPVLHDIKVKNNGIDISAPKGSSAR
                     AVYEGTVSAVITAPNGTTMIMIRHGEYLSVYSNIKTAAVKKGSSISSKQNLGPIAVDE
                     EGATVLHFEIWKEKVPQNPEYWIAK"
     gene            complement(37989..38831)
                     /locus_tag="PKY63_01380"
     CDS             complement(37989..38831)
                     /locus_tag="PKY63_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPE99291.1"
                     /translation="MSKFLLPVLLIAIAFTSACKTGREAKKEIKETVKQDTGVELIFS
                     KMKDAQFDFRTMNVKFQAKVESEKNNLGFGGSMRIIKDRTIWLSLSAMVGIEAFRIFI
                     TPDSVKMINRLNKTYFKGDYQLINELLKTPFDFDMLQALVTGNDFSYYENNIFKVGED
                     ARSYKISTPGRKKLKNYVANQSDMDRVLVQDLWISPENYKIIRQQIKEISKENSKLVV
                     DYSAFVVFDDQLLAHQIDVNVEAEQKMKVVIDFEKVSVDEEIMVPFVIPESFVPMDQQ
                     QKNK"
     gene            complement(38824..40545)
                     /locus_tag="PKY63_01385"
     CDS             complement(38824..40545)
                     /locus_tag="PKY63_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99292.1"
                     /translation="MSKVLAIYLLTLLLFSTACNSFHKAQRKHNKLSDRELLMISSAF
                     IEATRYKILGDAANAMGLYQTIIEQDPLHDASLYELARLKMLNGETDIAVDLLKTAIR
                     VDPDNIWYKLAYAGAFEKSGKYSEAAIQYKNLRNKYPEKYELYEYEAQCYVQNNEFQK
                     AIDVYNEIELQTGITEQIGIQKYYIYQNWSKTDKAMNEVQKLADHFPENPQYSMALVE
                     YYMRTGRAGLALNSINDVLTVDPENALAHAYLADYYHMSGNDSLAVKEIFYIIGSVST
                     GIDEKISLLMNVYRAGNVYGDTTVVYPMLDTLVSVHADEAKAWAMYADFLNADDRMDE
                     AMDMWKKSLEFDKSVFTIWNLMMNVFYDNQQYDSLLTYASEAAELFPEQSSVWFFLGL
                     AQYHAAMFEKAVVSLEFASDLRLSNKEMKNESLFLLADTYAKTGRNDDSDAMFEKLIG
                     EDPDNVVAKNAYAYSLALRKKNVDKAQQLIAEALTGNEDKAEFQHTYGLVLFRLGKYL
                     EAAEAYRKAIELGGDDDGALLEQYADVLFFQNKTEDALRFWLMARELGGAGKMIDQKI
                     TDKKYYE"
     gene            complement(40542..41546)
                     /locus_tag="PKY63_01390"
     CDS             complement(40542..41546)
                     /locus_tag="PKY63_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPE99293.1"
                     /translation="MKIIIPMAGMGKRMRPHTLTIPKPLINIAGKPIVQRLVEDLMEL
                     VDEKVEEIAYIIGDFGAEVEAHLLGIADSVGSKGKIYYQNQALGTAHAISCAAESISG
                     KVIVAFADTLFRTTQKMDTSREGIIWVHKVEDPSAFGVVKTDNKDIVTKFIEKPTSPV
                     SNQAIVGVYYFREGEVLRAEIEKLLAAKVLVNGEYQLTDVLENMMQRGCSFSVYNVEE
                     WLDCGNKNATIHTNRRILDFAKNGSLIANNLNQKNSIIIQPCYIGKNVTITGCIVGPH
                     VSIGEGSVISNCIISNSIIQSHCKISNANIDNSMVGNFVEYSEKYKELNIGDFTVIVQ
                     "
     gene            complement(41562..41996)
                     /gene="dut"
                     /locus_tag="PKY63_01395"
     CDS             complement(41562..41996)
                     /gene="dut"
                     /locus_tag="PKY63_01395"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPE99294.1"
                     /translation="MKINIVNTSNNPLPAYETEYSAGVDLRAFLDDEIVLKPMERKLV
                     PTGLFISLPAGFEAQVRPRSGLAIKSGVTVLNTPGTIDSDYRGEIKVILINFSDTDFV
                     IKNGDRIAQMIIAQHERAEWKLVSELDSTQRGAGGFGHTGKQ"
     gene            complement(42121..45348)
                     /locus_tag="PKY63_01400"
     CDS             complement(42121..45348)
                     /locus_tag="PKY63_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE99295.1"
                     /translation="MSKIDKHPILPVPECEKHIFLYNGVKVEGDKGFTIAAALHNAGF
                     PVHSHSLQDRNRSLECGIGKCGACEMLVDGVIRRICITLVDGVKEVKEVPKDYMPDEK
                     RNEQTQTKVYRTDVVIVGAGPAGLASREILNQHGVSNIVIDNNAKIGGQFNMQTHQFF
                     FFEKEKKYGGMRGFEIASALAGDNHEGIFLNCTVWDLLEGKRIAVKNIKTEEIFYIEC
                     NHLIVASGAVPFMPTFENDDVPGVYTAAVVQKMMNTEFTLLGKKILTVGAGNIGYLTS
                     YQLMQAGAQVVAIIEAQGKEGGFPVQANRVRRLGIPIMLNHILVSAIPNKDHTGIVGA
                     VIAEAENFKPVPGTERIIEGIDAINICTGLVPDDQLLILGNEIFGRNCHGVGDAIRIG
                     EGTSAVLRGKQAAYEVLDMMSVKYKYDDYLAVSKEYIDSQQHPVRVIEESALPVGERR
                     KKPFVQIDCLYGFACNPCTFACPHGAITKTSTSTVPVVDFDKCIGCMQCVYQCPGLAI
                     FGYNTEKDQLFLPIEYEANENEAVFLVDNNGKVLGEGVIEKILKKPNKTNVARVRAES
                     LHGEDIIQVRGFIVKSNYPEKPELQTTSYKEEGATYVCHCDDVTLDEIMDRVGDRKFI
                     SIDEIKHTTRLGMGACRGKRCIRRLKSTIASKGITVVGDATPRGPLSNQVLIGELYPG
                     KENELIVPFTPGKKTERRKVKSLVAGGGIGGSALFRYLAEAGFSPVLVNYDRGASWRN
                     IAGGRPAFSVPELSDIAIHNRDIFVELQKIKNVDYKPIRYVSFAHDDATFKALDASRA
                     WSDAYMVDKKDFQKEISPYFNKNLETYQSALITHDCWQATPGRVIDLVRSIGIEKGGT
                     VMEDCELIDLRREDASYIVLVRDHNKQYIEFETEVFINALGPTADKFARKLNIETGLY
                     PVRHQAFITRRLPLMGKDGDALDMLIDRRKYKSFSSVYGQQLKETGQIIGCASPLTDP
                     KETDKNLKINSKEFLEIVSEIFCDWIPQLSATGFQAVWSGYYVEPRYIVDPSLGLFVG
                     LRGHGFMLSQYLAKVYVDKLNGKPVPEYFEKLALSGPGLSESAFK"
     gene            45515..46459
                     /locus_tag="PKY63_01405"
     CDS             45515..46459
                     /locus_tag="PKY63_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE99296.1"
                     /translation="MGRITTFLSICIAFTIMACNGGNKDETKNNPQKSTVNNNLEAGK
                     ITIGATEESYDLAQRIASNYVAQNTNLIVEVIPCKINEAKQMIAENKVQLIVTGGNSN
                     PFPEYESTIIATDLLLLTVNFNNPALQYLVMRGLDLKDLSGIFCTGTINNWNQVDKKS
                     ESTPLKPLAGPDSSSSNNMVRSFLKSNLAPTVTSSMSENELTSSVSASPGSIVFMSHR
                     MTYNQNSGVRANGIYIIPVDFDGNNVANDSELIYDNLNMLKKAYNKGGFPKSLVRNHY
                     FIANGKAERSDIVKHFTHYAQKEASNVISGAGYFQPLK"
     gene            46468..47187
                     /locus_tag="PKY63_01410"
     CDS             46468..47187
                     /locus_tag="PKY63_01410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPE99297.1"
                     /translation="MKKILVILIIAAGLVSCHSTKKANTDSSAKMPQATIMTNFKDTV
                     SYIIGTDIAKSFSQNSIDINLDLLFEGIKDGYNGTDSLFTKQQVQEIMGKFQKEMQQK
                     QQLESAKQATLNKEKGKLYMDANAKNEGVMQTASGLQYKILRDATGPKPTATNTVKVN
                     YEGRLIDGTIFDSSYERGEPIEFGLGQVIKGWTEGLQLMSVGSMYELVIPSDLGYGDR
                     PMQSIPAGSTLIFKVELLDFK"
     gene            47204..47641
                     /locus_tag="PKY63_01415"
     CDS             47204..47641
                     /locus_tag="PKY63_01415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPE99298.1"
                     /translation="MKKLIMLLIAAAVVTACSTSKKDKETTASTENQTQEYITTASGL
                     QYKILRDAAGPKPGPTSRVTVHYEGKLLDGTIFDSSYDRKKPNTFGLDEVIPGWTEGV
                     QLMSAGSMYEFIIPPALAYGSRELPGIPANSTLIFKVELLEIK"
     gene            complement(48031..49173)
                     /locus_tag="PKY63_01420"
     CDS             complement(48031..49173)
                     /locus_tag="PKY63_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPE99299.1"
                     /translation="MASCFTWFYVSNKFPHLISYFKFLISYFVFYYLRISMVTQNKIS
                     FLKYFSTWRILIPIALGLSISAYMIGRSFDANMLSQLDWGGKTIFWIFIAVLCLLLRD
                     FMNMLRLRILTQKKLSWNQSFQNIMLWEFGNTVMPAFIGGAGVALITIHKTGVPLGRS
                     TAVVMISSLLDELYFIILIPLLLLVGYIAGIYGNSLFAVDLKPDWIVWGAYIYLASLA
                     FFLSVSIFFLPTFFAKILRRIFSLKFLRRWKGRANRMGSDLKASSREMRGKSFGFWIQ
                     LFFVTLIAWVSRFFIINCLIMAFMPGVDHFELYYRQLYMWVIMLASPTPGASGIAEWI
                     FSDYLGMFVIVGLAPVLALIWRLLTFYYYLLVGSLVMPVWIRRVFK"
     gene            49482..50120
                     /locus_tag="PKY63_01425"
     CDS             49482..50120
                     /locus_tag="PKY63_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPE99300.1"
                     /translation="MPIRKVLLILLVPLMFSSCTGLREHIRQRQLETHGPPKSIRDNR
                     NDRTERKDKTRRDTLSNITERNAPTGIYAKYGEKWKVELSGTEDVKFLEEIDSWLGTP
                     YVYGGEDKKGTDCSGMIMTMYKTLYNLSLNRSANDMQKNVRFIDIDKAKLGDVLFFKI
                     NGDRVSHVALYLGDRRFIHATTSKGVIISSLDEDYYSKRYFKCGRIIAMERQ"
     gene            complement(50554..51009)
                     /locus_tag="PKY63_01430"
     CDS             complement(50554..51009)
                     /locus_tag="PKY63_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE99301.1"
                     /translation="MEKFESMDHILDFAMNQEQSAVDFYTKLTAKSRNDEMKQIFEEF
                     AREEIGHKAKIAQIKEEQNFDLPKEKVMALNIADYLAYPDVDENITYAEALKVAMARE
                     KAAFKLYTKLASEASNSAIRNIFQYLAQEESKHKLRFELEYDEFVLRDN"
     assembly_gap    51207..51306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<51307..54308)
                     /locus_tag="PKY63_01435"
     CDS             complement(<51307..54308)
                     /locus_tag="PKY63_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE99302.1"
                     /translation="MDTINVVLNGKNVQAKQGETILELATRYGYHIPTLCHDPRLEPF
                     SSCFVCVVHVEKMKGLQPSCSTKVTEGMVIDTEREDVKKSRKMALELLLSNHYADCIG
                     PCKETCPANVDVQGYISLIEKGLYSEAIGLIKERNPLPAICGRVCVRPCELACRRNLL
                     DENNGVGIDYLKRFAADQDLKSDNHYRPEVAPATGKSVAVIGGGPGGLSAAYWLAQKG
                     HQVDIFEAMPHAGGWLRYGIPEYRLPNAVIDQEISTITELGVNIYTEKKLGENISYKE
                     IKEKYNATILTIGSQKGTLVGVEGEDADGVFSGIDFLKNMEATGQRYDFKGKKIVVVG
                     GGNTAMDCCRTSRRCGSEEVYVVYRRTEAEMPANPIEIHESKLEGVNYMFLTAPVKIL
                     KDENGKLKSMVCQKMELGEPDASGRRRPVPVEGSDFEIECDYILAAIGQKTDVNFIND
                     INEFATGGQLKITKWGDIEANKETLETGIPGVFAAGDGVTGPATAIAAIAQAKLAVHS
                     CEQYLNGDAIVPPAKEFFSRKENFRKQEKPEYVGKFKHQLREEMPVLDPKDRMNFSEV
                     ELGYASEEIAKNETARCLECGCGALYTCDLKKYASEYNANQVHYSGSFNEYKIDFSHP
                     YIEIDNNKCILCGRCIRICKEVVGAEALGFVNRGFETYVAPAMGMSLKETKCESCGMC
                     VSTCPTGAMSENKLFKPGPVKTESFKTICNYCSVGCELEVQHRGGFVYGVKGSKGQVN
                     QGGNLCNSGRFGYQYMNDENRLLKPRVKENGSWKDIEWSEAFEIIAKQLKSSKPSESA
                     FTVGARLSNEEQYYIQKIARAGVKTNNISSFHYMERGKGYTENSMASVPFNELKFARA
                     IWVLNPEMIKSNGVAGFAVQVARNKSEIPVTLIIEGDNHLMDHKADRIIAVKSRYAFI
                     KAMNHYLLTSGKQNALFINGRTEGFDEYKQTLLKESYASLIAASGLAQDALESLADEF
                     NHIAETVLIYTEAGTSSNCALEINNLML"
     gene            complement(54327..56105)
                     /locus_tag="PKY63_01440"
     CDS             complement(54327..56105)
                     /locus_tag="PKY63_01440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPE99303.1"
                     /translation="MAQTLVRVGMASCGIAAGARKVFDTIKGLQNTDHLKFDLQQTGC
                     IGMCFREPLVEVIDENGSYLYGEVDAEKALEILNKHVRNGEPVREYIVRTDLFVSKDD
                     SFWKGQVKIALRNCGVIDPENMDDYIRQGGYEAVKKIFSSSISREDVIQMIIDSGLRG
                     RGGGGFSTGMKWKFAYNSKNETKYIICNADEGDPGAFMDRSLIEGDPHAIIEGMIIGA
                     YAIGASDGVIYCRAEYPLAIKRLNIAIGQAHERGYLGRNIFGIEGFNFDLYIKEGAGA
                     FVCGEETALMASIEGERGMPRRRPPFPAVSGLFKKPTNINNVETWANVSWIILNGPEK
                     YAAYGTEKSKGTKVFALAGKINHGGLVEVPMGIPLRDVIFKLGGGIQNNKKFKAVQMG
                     GPSGGCIPEHLLDTIVDYDSVTATGAIMGSGGLVVMDENTCMVDMARFFLDFTQKESC
                     GKCTFCRVGTKRMLEILTRITEGEGRDGDIELLEELCEQIKTSSLCGLGQTAPNPVLT
                     TLKYFRHEYEAHIYDKKCPAHSCKKLLTYTIDSDKCTGCTVCAKNCPVNAISGERKLP
                     HLIDQSLCIKCGVCFSKCKFDAIILS"
     gene            complement(56114..56635)
                     /gene="nuoE"
                     /locus_tag="PKY63_01445"
     CDS             complement(56114..56635)
                     /gene="nuoE"
                     /locus_tag="PKY63_01445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE99304.1"
                     /translation="MRIERVPVAHVETKAPEVDLSLIDSILHHYQNHKGAVIPVLQKI
                     QDVYGYLPSVAIVKAAETLHIPVSDIFGVATFYSQFRLHPVGKHIIKVCHGTACHVQN
                     ATAISDAIEEALEIKDGQTTEDGLFTLESVACLGCCSLAPVMMISGETYGKLTGKSAV
                     KIVNDIRMQEKQK"
     gene            complement(56995..58332)
                     /gene="gdhA"
                     /locus_tag="PKY63_01450"
     CDS             complement(56995..58332)
                     /gene="gdhA"
                     /locus_tag="PKY63_01450"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPE99305.1"
                     /translation="MNVKQIMQDLEQRNPGQVEYLQAVREVLETIEEVVNENPQFDKA
                     GIIERLVEPDRTLMFKVPWVGDDGKVNVNRGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGAKGGSDFNPKGKSDGEVMRFCQSFMLELWKLIGPETDV
                     PAGDIGVGGREIGFMYGMYRKLARENTGVLTGKGINWGGSLIRPEATGFGGIYFVEQM
                     LKTMGTDFKGKTVSLSGFGNVAWGAALKATELGAKVVTVSGPDGYIYDEAGISGEKIN
                     YLLDLRASNQDIVKPYADKFPGSKFFAGKRPWEVKVDIALPCATQNELNGEEAKMLIA
                     NGVKIVAEISNMGCTPEAVEALHAAKIPFGPGKAVNAGGVATSGLEMTQNSMKLSWNA
                     KEVDERLHSIMNNIHAACVKNGKEADGYINYAKGANVAGFLKVANAMLDQGVV"
     gene            58537..59643
                     /locus_tag="PKY63_01455"
     CDS             58537..59643
                     /locus_tag="PKY63_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPE99306.1"
                     /translation="MNTLGLHDRAKELNCLYKVDEVLQFIDKEVPQAISELLDVIPSG
                     WRYSDLCEGEIILGDHIFSRPGLQRTELKQSALIKAYGEIIGEIRVYYTKPVKSEKGI
                     FLAEEMQMLHSIAEKIAMFVTLRKLKPSHLEEEASEDEAEILKNLQHDFPEMMDWLKN
                     MGLKKREAVQILRNPLNFRKGETIGKQGAFTSYFILLAKGATKSYIEGPGSKSYSFML
                     TLPFEFIGMSSLFGNHYYFSTVALIPSTVFLIEKEKVIKLINENPKFNKSIVAWLCDS
                     YKILFHKMSCLSLKQTIGRMAEVILYLSNDVFKNEVIPHYISRKDLAELSGMSNENAV
                     RILSDFKNENILRDTPEGLQILNKSLLQTISITG"
     gene            complement(59840..>60540)
                     /locus_tag="PKY63_01460"
     CDS             complement(59840..>60540)
                     /locus_tag="PKY63_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPE99307.1"
                     /translation="RLPGRQEEMIHKIAETGKQVIVVLVGGGPVVVTDFIDDIDGLVE
                     VWYPGDLGGTAVADVLFGAYNPAGRLPISFPVDEAQLPYLYYHKPTGRGDDYLDMTGK
                     PMFPFGFGLSYTTFEYSDLKFSKDSFSSNENTMVSFKLKNSGKVDGDEVVQLYIRDEL
                     SSVARPMIELKGFKRVHLKAGESKNIAFEISPEMLSMLNEHMQRVVEPGSFRIMIGAS
                     SNDIRLRGILTVED"
     assembly_gap    60541..60640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<60641..61842)
                     /locus_tag="PKY63_01465"
     CDS             complement(<60641..61842)
                     /locus_tag="PKY63_01465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPE99308.1"
                     /translation="MRSFFLIVILFFAVSEIPLRAQDALPYKNPRLSTEARVADLLNR
                     MTLEEKFWQLYMIPAALDVDLEKYPHGIFGFQFAAVGQAGNGAEQMMNYSGVGPVETV
                     KAINRAQKYFVEETRLGIPIIPFDEALHGLVRKGATSFPQSIALAATFDTALMADVAE
                     QIAFEVKERGIRMILSPVLNVAVDARWGRVEETYGEDPFLVTQMALAYISEFEKQGII
                     TTPKHFIANSGDGGRDSYPVQYSERMMEEVFYPPFKAVIEKGLATSIMTAYNSFNGKP
                     CSQSHFLLTEKLKNEWGFRGFVISDAGATGGANVLHFTAQDYADATKQSIEAGLDVIF
                     QTSYDHYTLFYEAFQKNLIDPKAIDSAVARVLRVKFELGLFENPYVEERILRDVPLPD
                     LPLNAARES"
     gene            complement(61849..>62799)
                     /locus_tag="PKY63_01470"
     CDS             complement(61849..>62799)
                     /locus_tag="PKY63_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPE99309.1"
                     /translation="EAFRNKGFMVGAYFSKPDWHCPAYWTPLLATPDRNCNYDTRKYP
                     GRWKEFQNYTYNQLEELTTEYGKLDILWLDGGWVRPDSTINEEVRSWGYNIPKYEQDI
                     DMPRIAAMARENQPGILIVDRTVHGPFEDYRTPEQSIPDSILPYPFETCMTMTDNWGF
                     VPNARFKSAETIIRSMVDVVSKGGNFLLNVGPTPKGTFEEKAISTLNEIGKWMSVNGE
                     AIYGSRSWRHFKDGDAIRFTQSKDGKYLYVFALTWPGKSLTLSCITEKKNYKVQMLGS
                     KEKIKTSFSADGYTLRMPDSMQDLAKRPTAYISVFRITLK"
BASE COUNT        18600 a        14225 c        12402 g        17372 t
ORIGIN      
        1 cagccatcaa tctgcactta gacacattgg aataaaatat atttcacgat ggtattattc
       61 atactgtggc gttaatgcga ttatgcgaaa tttgatttgt cgcatcatcg ccacatcggg
      121 tcatcgccac acatatattc tgttttcaca ctctttctgt tggattatga gtcaatcact
      181 gacattatta aggatgatca gatttatgaa taaggatttt tgatttttga ggtgtttatt
      241 ttgtaaatac aagattttca atagttatga gttatgagcg atgagttata agttataagt
      301 gaaaatttgt aacgattgca tcgttcgtaa cgcgtaaagg aacagcttct gctcagagca
      361 ccgacgcttt ggtcggcacc tactgctggc ggctgattat cccatgcctt gcagacgtga
      421 cttcgctgac tactgagaac ttattggcta attccggaaa tattgtattt ttgaacaaga
      481 aaacagtgca tgacttcaaa aaaacagatt ggactttttt tcggatcatt caatccgccg
      541 cacatcgggc atatggctat tgcaaattat ctgcttgctt tcggaaacat cgatgagctc
      601 tggtttgtag tttccccgca gaatccgctg aaagagaaaa agtcgcttat tcagggacgc
      661 acacgacttg agatggttcg acgagccatt ggaaattatc ctggtatgcg tgtcagcgat
      721 atagaatttg gcctgccaac accgaattac acaataaata cactggttca tctggaggaa
      781 aaattccctg attatgcatt ctggcttatt atggggatgg acaatatcca gactttccat
      841 aaatggaaat cgtgggaaac cattctggaa caaaacaaaa ttctggtgta tccacgtcct
      901 ggctttgatg ggaacccaca tgtaaaacat ccgaatgttg tattgatcaa tgcaccactt
      961 atggaaataa gttccagttt tattcgcaaa gcaatcggag accgaaagaa tatgcggtat
     1021 tttttaccag aaacaatttt tgaaatgata gaaaaagaag gattgtacaa aaaacaagaa
     1081 atatagcatt caaattccaa tattcaagaa aaggaaaaga ctgccaaatc tgacaaaagc
     1141 cacgggcata aacaataaaa agtgacaatc tgccgtgttt tcaataaaaa aggtcatttg
     1201 aatcatttaa cggcaagtca aaatattttt tagggtcgtc tctgtttcat tttttttgta
     1261 ttttagcagc cggtttcagt aggagagatg ctcgagcggt tgaagaggca cgcctggaaa
     1321 gcgtgtagtc gcctaaagtg gctcgtgggt tcgaatccca ctctctccgc aaaccaacaa
     1381 gaaattgaaa acaaacaaat aacaaactaa acgaaaatca aatccattat gaaaaagttg
     1441 atttcactgg ttgccattat gggagtactc tttttcagta ctacccaggt ttcatttgcc
     1501 caggaagcaa acaccgacac cccaacgaca gaagttgttg actcaacagc atcaaataca
     1561 accggaacac aggtaacacc tgccggcggc gaagaagttg ttgcagacaa aagtttccat
     1621 cagatattaa aagaaaaatt catcgaaggt ggtgccacct ggatgacccc gattcttatc
     1681 tgtcttatcc ttggtcttgc actcgttatt gaacgcattg tttatctcaa catgagccgc
     1741 accaacacag aaaagcttct gcagaaagta gaagaatcac ttcaggccaa tgatattgaa
     1801 ggtgcaaaag aaatctgccg caacacccgc ggtccagtcg ccagcgtttt ctatcaggga
     1861 ctcgaccgat tcaacgaagg aatagaaagc gtagaaaaag ctattgtagc ttatggtggt
     1921 gtgcagatgg gccgtcttga gtcgaatctt tcatggattg gacttttcat tgctctggct
     1981 cccatgctcg gattccttgg aacagttatc ggtatggttc aggcattcga cgatatcgaa
     2041 gctgccggcg acatcagccc aaccgttgtt gcaggcggta tgaaggttgc tcttatcacc
     2101 actgtgtttg gtcttattgt agctattatc cttcagatct tctacaactt tgttctttca
     2161 ctcatcgaaa aactcgttaa tgatatggaa gatacttcca tctcatttat ggatatcgtt
     2221 ttccgcgcaa acaacactca aaaacagtag tctcatgaaa tcagtaaaaa gtatagcaat
     2281 cattgcaata ttgattctgc ttgctgctat cacgcttttt gcaggaattg acctgagact
     2341 atatgtcact tatgcaattt ttgcaattgc catcctcgct ttggttgctt tcactgccta
     2401 ctcggcagca accagcggca aaaaaagcat gacaaccatc attctgggtg cagctttggt
     2461 cggaattgta ttgttgttct attttatcac accaaccgac gatgtacctg tcgcattatt
     2521 tgaaaaaaca ggcacatcaa tgggctggag tgcaattatc ggagccggat tattttcaat
     2581 ctacgccctg ctcggtattt ttgttgctct gctcgttttc ttcggtgtga aaaacatcgt
     2641 taataaataa tcaccaaatt atggcacgaa aagtccctga actcaatacc ggatccatgg
     2701 cggatatatc atttattatt ttgatattct ttctgcttgt gtccaatatt gatacagatt
     2761 tgggattagc gagaaagttg ccaccgatgc cggatccgga tgccgaagta cccgaagtca
     2821 acgaaagaaa cgtttttgtt gtacttgtca acgcatacga catgctattg gtagaaggta
     2881 agcctggtaa catcaacact ctccgcaagg aagcaaaaaa attcttcctc aatcccacaa
     2941 atgataaaaa cctctccggt aaagaaatgg taaacattcc tttcataggt ccctacgaaa
     3001 aatccaaagg cgttatctca ctccagaacg accggggcac ttcctatgaa atgtatgtaa
     3061 aagtacagaa tgaactggct gcagccatca acgaaatgcg cgacgatctt gccaaaagta
     3121 aatttggtaa acctttctcc gagctttctg aagattattc caaagctgtt tccaaagcca
     3181 ttcctgttgc catttccgaa gctgaaccta aagatatagg aggcaaaaaa taatggcaag
     3241 atttaaaaaa tcaggcaata aaagtgttcc aggtattaat accggatcaa cttcagacat
     3301 cgtttttatg ttcctgtttt tcttcatggt tattacaacc attcggcagg ccactctttt
     3361 tgtacgtgtt tcggcaccta atgccactga agttcaaaaa ctcgaacgaa aaaacctggt
     3421 ctcttatatc tacatcggaa accccataaa tgcgaaaacc tatggctctg aacctcgtat
     3481 ccagctcaac gatcagtttg caacatcaga cgatgtagcc gaatttatcg aaaaagaacg
     3541 ggctgcacga aacgaagctg accgtcaatt cctcactact tcgctcaaag ttgacagaaa
     3601 agtcaaaatg ggcgttgtta ctgatgtcaa acaggaattg cgaaaagtgg gtgcttttaa
     3661 aatcaactac tctaccaaaa aaggaaaagt gactgactaa gtcaccgact tatttgaatt
     3721 cagaaacagc tgtcatcatt catggcagct gtttttattt aatgactcag ctatcggagg
     3781 gttcaaaccc ttcgatggat ttacgaggtt tttcgctaac tttgtatctt aatgaaaagc
     3841 atttcccttc aggaacatat caatcaaagc cgcattgctg ctgccgcaaa ccgcgccaaa
     3901 ggaaaccggc acggacaaat actttccatg ctgtattccg atccttatca ttttgttgaa
     3961 gaactgatac aaaatgctga agatgctatc gcacgccgca aaaatgaatc tgtttcaggt
     4021 tttgtgaaaa taattatttc tgaaaccggc atttcatttt tccataacgg cgatccattc
     4081 agcgaaacag acctgatggc catcacaaca tttgcttcaa caactaaaaa aggattgccc
     4141 ggaatcaatc agatcggaaa atttggtatt ggcttccgct ctgtgtatgg cattaccgac
     4201 aatcccgaaa ttcacagcgg agattttcat tatcgcatca gcgatttcga agtcctcgaa
     4261 gaatgtgctc cgcgccatgc agaagcgttt acaacattca tctatctgcc tttcaaacca
     4321 tctgtcagtg cggacttccg aaaactactc caaaacagaa tctgcgcgct caaccctacc
     4381 tttctgctgt ttctgaaaca aattcagcaa atagaaatta ttgcagaaaa ccaatcggta
     4441 gttattaatg cagcaaccga actatgcgaa aaaaaaatca cccgcaaaac catcgtttcc
     4501 agcaataacg gaataagaaa cgaattacac tttctgttga tgcagcgaaa cagcggtcac
     4561 aaccacaaag cagccatcgc tttacaaata aatgaatccg gtcattttat accaataaga
     4621 aaccctcagg tctcggttta tttcccgaca caattccaca taacacattc catcatcgtt
     4681 catggcagtt ttacaactac gccgaatcgc gaaaatattc cattctccga agaatggact
     4741 cctgaaaatt atgcagtttt atctgttata tccgatttaa tgcggatttt actacgatca
     4801 ttgctgaata agaaaataat taccgctgat ttctggtcac tcttttcatg gaacagctct
     4861 tccgaagatc ctgtctcagc cgtcatttcc gagtgtctga ataaattcat cgctcaagaa
     4921 aaatgcctgc ccgatcaatc agggaaacta caatcagtgt caggactgtg tgtcagtgaa
     4981 gatgataatc tgattcagct tattacggca aaagacatta tgaatgttta tggccgctct
     5041 ggattcctac attatgatat cactgacaat gaaatctttg tgtccttttt gcgcaaatcg
     5101 cataaactaa aagtggccga cattgattca ttcgcttttc atattgcaag tcaaaaagaa
     5161 tttttgcaga aaaagacact tacatatttt ccttcattgt atcgttttct cgcactgcat
     5221 ccacgtttat gggaccatac acacaaaggg cgctactaca atcttcgaca caaagccatc
     5281 attcccgaca aaggaaagca tcttacagcg ccattttccg acaatgataa acctcagatt
     5341 ttcattggaa aagcagccag cggattttcg gttgtacatc cggaattgtc ttccaatgct
     5401 gactgcatga actttttctc catgcttggc atcccggatt ttgctccggg actttccgaa
     5461 gccaataatc tgttccggcg tttcaaagaa aagaccgcct cagtctggtg gcacaatctg
     5521 tatgaaattt atgcttcatc cgaagccagt gtaaaagacc atatccgcga aaaaacaaac
     5581 gaacttaact gcctgcattg cattgattcg caaagtggaa ataaaatatt cgtgcagccg
     5641 caacaggcat acctcgctga tgagcacctt actgcatttc tatcaggtca acaatctttt
     5701 tttgttaatc cgcaactgat cgcatttatg tcggatcaaa acattgcagc ttccagcttc
     5761 ggtcattttc tgacagagct gggagtcaat gctgcactcc gaattgttga aacagagtca
     5821 caactggatg acaatcacaa atcggcacta agagccggct atgaacagtt ttccattgtt
     5881 cacgaaacaa tcacagattg gacaattgaa ggacttgact cgtttctcat ttcgccaaaa
     5941 ccagcatcag cagccgcttt gtggcatcta ctggggaaag tggatgaaaa acaccgcaaa
     6001 gcctgctata catttgaatc ctacgtacga agcgaaacca tacaatttag cccaacttat
     6061 attgaaaaat taaaaacaaa tgcctggtta tttgacgagc atttgaatgc cgtttcaccc
     6121 caaggttttc agatggaacg acttcatgaa agctttaacc aatcgaagcc ggcatcgttg
     6181 tggatggcag cagaactggg aatgattact gaaaatattt caagtgaaga aaaagaaata
     6241 ttgtctctta taagaacaca cagactgaat acagaattgc tgcaacaatt acttgacgac
     6301 acaaaaaacg atgtggaatt ttttcaatac ctgcctatgt cagaacccgc atcaattgca
     6361 aaaaacacga tgaatttttc aaccgcagat ttgtcagcaa tcattcagtc aaatcctttt
     6421 aacgcaaaca attcgcaaag ctgggaccag tatctgtttg aagagaaaac acaaatcctt
     6481 aaaaatcatt ttctaaacac agtgctttct gcatcaaagc aatcgaagcc atatattgca
     6541 aaacccggac atctgcaaat taaacaaaac gaaattataa aagctcatta tttcacagga
     6601 atcaggcctg atgctggcga agaaattctg ctgccgtcat catttgcaaa tcattttaaa
     6661 aatgagtctg aaaaaaaaga tacaggatta tttgttttca acttgcatga caaaacctgc
     6721 attgaagtat cacccgataa tctcgcagaa tttctgaatt cacattccat cgcattcatc
     6781 aaaacaacaa aataaaatcg gatgacagag cttgattcat ttacaaaaaa cctaagcact
     6841 ctatgcccgg atttcaggca gaaaaaatac ttactgggaa tcagcggtgg aattgacagc
     6901 atggttatgc tgcatctgtt tcaaagagca ggattggatt gcagcattgc acatgtcaac
     6961 tttcagctgc gggattcgga aagcgaccgc gatagaaatt tcgtttttga agaagccgca
     7021 aaagcaggaa tccacattct ggaaaaaaca tgcaacacaa agcactttgc acaaaaaaac
     7081 cgaatgaata ttcaggatgc tgcccgaaaa atcaggtatg atttttttac cgaaactgcc
     7141 gatataaaca atttcgattt tatctgcaca gcacacaacc tcgacgatgt ggttgaaaca
     7201 ctcattatgg gtctgaaccg gcgcggaagt ctgaacacac tatcaggcat ccgcgaaatg
     7261 gaaaaccgga ttttgcggcc attgctgcat ttttcgcgca gcgacattga atcatacgca
     7321 gccggaaaca tggttcgata tgttgaagac agctccaact caagcgacaa atatctccga
     7381 aacaaaatcc gccatcattt gctcccattg tttgaagaaa ttatgccagg attcagcaag
     7441 cgggcagcag ggtccgtttc attccttcgc gaatatcaaa aatttgttgc ctcggcggct
     7501 gcagctgaat tactcaaact gggcaatgcc caaaaagaag ggattgattt attgcaattg
     7561 cagaatcatc cctcaggcaa attgctgctg atgcaattct tgctaagtca tggttttttt
     7621 cctgccgatg ctgcagaaat tttcagaatc gacaattatt ccgagccaaa agaatttctt
     7681 tccaatgata aaaaactcat tgttcatcga ggaaaaataa ttctttcaga aataaaaaca
     7741 ttcgaagaca accagatatg gtacatcgat gaagatatga acacagatca tctgccggtt
     7801 ttgctgcaga tggaatactg tcagatcaac agaaaagaag agctgatttg caaaaaagac
     7861 actgctctga taaaccccgt tgaaataaat tttccgctat tggtcagaaa atggattcca
     7921 ggcgatcggt ttatcccttt tggaatgacc cagttcaaaa aagtcgggga tttctttaca
     7981 gatgcaaaaa ccacagtcgc agagcgcaat tcaacatatg ttctatgcag cggtcctcac
     8041 attatttgga ttatcggata cagaaccgac caacgtttta tgataaatga cattccagcc
     8101 accgctttaa gaataaaaac gcgttgaaca ttttcttatt tttgctgctg aaaatttaag
     8161 tctaactcta tgattttacg ccgatttcct cttttctttg ccatgctgct gttattttca
     8221 gcagttcaaa gtcagattat taaaccggta aaatggaaca gccgggtcga taaagtttcg
     8281 gataccgaag ccaatatcgt tcttactgca agtattgatc agggatggca tctttattca
     8341 caatacaatc ctgaaggcgg ctcactgcct ttggttttta caatctcacc ctcaaatgcc
     8401 tttgctcttg tcggtaaggt agccgaatat ccaaaaccaa cagagcacta cgaagaagta
     8461 tttaaaatca cagaaaaatt ctgggccggg aaggcatatt ttacacaaaa aataaaggtt
     8521 ttaacagaca agccctttga cttcgaaatc agtctcagcg gtcaggcctg tcaggacgac
     8581 ggatactgcg tgcagattga agacgatctg gtggttaaag ttgatggcag ccagtacaac
     8641 aaagaaaatc cggttgatac aacggttatt gcagtcgttg ataccggcaa aataaaacct
     8701 gacaccagca aaacggaaac agcaaccctg tcgcccgata tagaagatgc tgaaaaagct
     8761 ggcagcggaa tatggggcgt tatcatcgaa gctattctct ggggttttgt tgcactgctc
     8821 actccatgcg tgtttccaat ggttccaatg accgtttcct tctttctgaa aggcagcgaa
     8881 aataaagcta aaagccgctt caacgccagt gttttcggca tcagtatcgt ggctttatac
     8941 accattccaa ttgccatttt gattggagta acctattttg tcggagggca aagtataaca
     9001 actgatatct ttaatttttt aagcactcac tggattccaa acgttatatt ttttctggtt
     9061 ttcatcattt ttgcagcatc tttctttggt gcctttgaaa ttgttttgcc ttcgtggatg
     9121 acgtccaaag ctgattcaaa agccgatcgc ggtggtattc tcggatcatt tttcatggct
     9181 cttacgttgg tattggtttc gttcagctgc acaggaccta ttgttggaac cattatcgtt
     9241 aaatccacac agggcgaaat ctgggaaccc attatcacca tgctggcatt ctcggcggca
     9301 ttcgcactgc cttttacctt attggctttt ttcccaagtt ggatgaaaaa tctgccaaaa
     9361 tcaggcggct ggctcaactc agtgaaggta gtgcttggtt ttattgaagt tgcgctaggt
     9421 ttcaaattcc tcagtgtggc cgatcaggtc tatcattggg gtattctcga ccgcgaaacc
     9481 tatctcgcca tctggattgt aactttcacg ctgcttggtt tctatctgct tggaaaactc
     9541 aaattcaaac acgacagcga agtgaaatat ctcagcgtgg gccggcttgg aatggctata
     9601 gccgtgtttg cttttgttgt ttacatgatt ccgggtatgt ggggtgcacc tttacgtgtc
     9661 ctgtcgggat atctgcctcc gcaacagacg ctcgattttg atatgccacg cattgtgcgt
     9721 gaagcggcta tgctgaatgg cggcggaagt ggtgaaccca caaagctttg cgaaaaacca
     9781 aaatttgaag acattctacc ttctctgcca catgggctcg aaggttatca cgatttcgat
     9841 caggctctca aatgtgcaaa ggaacaaaac aagcccctgt ttgtcgattt cacgggccat
     9901 ggctgcgtca actgccgcga aatggaagcc aatgtctgga gcgcgccaga agtgcttaaa
     9961 cggcttaaaa acgattacat tgttgtggca ttatatgttg atgacaaaac cgaacttccg
    10021 gaatcggaat ggatcacttc gacctacgac ggcaaagtga aaaaaaccat cggacgtaaa
    10081 tttgccgatc tgcaggtaac ccgctacgaa caaaatgcac agccctatta ctgtctgctc
    10141 gatacagcag gaaaagacct ggtgaagcca cgtgcatacg atctcgacgt agacgcattt
    10201 gtacagttcc tcgacaacgg cctgaaagaa ttcaaggcgc ggcaggggaa ataaaaacaa
    10261 tcgaaatact attctgaagc aggctgcggc ctgctttttt tattttagga tgtttaatgc
    10321 caaaaggaaa tatacaactg tccaaagatt tggatgactc cggaggagtc gcatgtctat
    10381 agaaaacaat aagaataaaa cacgactcca gcggagtcga atgtttttca agctgtttcg
    10441 cacaggtagc cgtttacatt cgacctctct gaggtcgtga ctggtatata catttctcta
    10501 tagacatgcg atgcctctgg catcggccat caaattcgca tacatacatc ggcctaaaat
    10561 atatttcaca acggtataaa ttaaaatcag caaataaaac tatttgatat atttgtgttg
    10621 caggtgcccg acgtttactg cataatatcc caaatgtctc atgaatataa ctgttgaaat
    10681 tattattgca ggaatgcttg ctctgtttgt tttatttgag acaatcctca ttaatatttt
    10741 caaaaaagat aaaaatgcat tattaacagg aatggtaagt ttgctatccg ttttgtttgg
    10801 gctgtattta tgtatgaaaa atggattaat tcttgactat gggacagagc aagaaacaat
    10861 aaaatcgcat gccttcacaa tgtacgtttt aggagttgca aacttttgca tcggattact
    10921 atttattgga atttcattta ttaaatatat catttggcaa acgagaaagg tcaaaaataa
    10981 aagcagaaaa taagaaggta cactttgatt aatcaatatc tcaaagcaga agttcctcag
    11041 aaactattcc tctcgatcaa tttctcaatc cagttgataa tagatattgc agtcaaaaaa
    11101 ttgtaacttt gtaaaaaaac aatcatgagt actgtcgaat taaggcatac tataattgaa
    11161 atgctatcaa aaattgatga cacctcgttt ctgaaagcca ttaagactat tgtagaatca
    11221 aaggcaaatg aggatgtcta caaattatcc gatttccaga aaaaacgtat taaagctggc
    11281 cgcgaacagg ttaagttggg acagaccatc tcaaatgatg ctttacaaaa ggagatcaaa
    11341 caatggctaa gtacaaaata gtttggtcaa ttgaggccag acttgacttg cttgatatac
    11401 tcggcttcta cattgaaaga aataaatctg tttcctacag caccaaatta aacgataaaa
    11461 taaataaaag tcttaatcag attgatcaaa atcccttaat tggtattaaa acggaaattg
    11521 aatcagtaag agctttgata accggacact atcagattat ttatgaaata caagaacaaa
    11581 caatcgtagt agtaatgatc tgggattgcc gccgcgatcc tgaagatatg gttatcgacc
    11641 tgcgcaccaa tcaataaaaa agtaagaatt cagaaaagaa ttttagttat acacctctca
    11701 gaaactattc cgctcaatca gtttctcaat ccagttggcc ggtgcctgat aatcaatgtg
    11761 gagcatcttg tcgaaaagcg cttcaggact gacatctgac actatcgcct tcaggtgatc
    11821 ctcgcgcaca aatttttctc tgactgcatt ctgcagcatt tcgagcagcg gattgtagta
    11881 gttttttgta ttcagcaatc cgatgggctt catctgcaaa ccaagctgca cggcggtcgc
    11941 cacttcgaac aattcatcaa aggttccgaa accacccggc aaagcaatgc aggcatcagc
    12001 tttttcgaga atcgtggttt ttcgcgttgc catcgagtcc gttacaatca gctctgagag
    12061 ccctttgtgg acaacatttt ccgacataaa aaaagaagga accactccgg tgatatgcat
    12121 cttcaggcga agtgcctcct cggcaacaat gcccatcaga cctgtacagc cggcgccggt
    12181 aatgatggaa acattgtgtt tgtgcagaac ccggatgagt tcttcggcac catcagcata
    12241 tattttgtca gctccggccg atgagccgca gaaaatagct actgtccgga tatctttctt
    12301 ttcattcatg ctgcaaagat atggattgca gaaaaaaaaa cctgcaaaaa cacgacgtta
    12361 tcgccatttt atatttattt ttgcactcac aaactcttgg tagctcagct ggtagagcaa
    12421 ttgactctta atcaatgggt cgggggttcg aatccccccc gggagaccaa agccactctt
    12481 attcgggagt ggcttttttt ttagggcctg ctgaaaaagt ctgaggtgcg ccagatttga
    12541 ggcggcaaga cgaagatgta tagtaatact tcgagtcgat ggcaacgaag aagatggcgc
    12601 gcatcagact aataccgtgc atatgaaata agcattgaaa cggcataagc aaacactcac
    12661 cgcaatagtt atgatattat tgaaattgtg agtgtcgcgt gtttttcagc aggccctact
    12721 tcgccggtga tgcaatacct gcaatgctca cccgcacgcc ttcgaccgcc agcagatgct
    12781 ttgcacaggc ctcaatggta gcgccggttg taatcacatc atcgaccagc agcacatgtt
    12841 ttcccttgat tttatcagga ttccgcacag cgaacatgtc tttcacattc tgatagcggt
    12901 cccagcggtt tttacgtgtc tgcgtttcgg tgaattcaac ccgcactaaa acatcctcaa
    12961 taatttcagt cggatatccc tgaataaagc catcagcaat gactttactc tgattgtagc
    13021 cccggatttt cagttttttc ggatgcaatg gaataggtat aaccgcatca atattggcat
    13081 acaaagccgc ctctgaaagt atttttccga gccggcgccc aaaatactcg ccaacctccg
    13141 gcctgttctt gtattttaac tgatgcatca tgtgttgaat caatccacct ttatcataaa
    13201 agcacatggc agtggcattt tcgatgggca actttccgaa aaagagctgt gcaacaggat
    13261 tatcggcata agtatgaaaa tctgtttttg caattttgga caggcaggac aggcaaatga
    13321 ctttttctcc ccgcatgaga ctttcgccac aaccgcagca taaatgagga taaaacaact
    13381 gtaacaatga atcaaaaatc atcaatcaaa taaatttgcc tgtttttcgt tcaaaagtaa
    13441 taaatttgca gttcatttca caagtacaat gttttatgga aaacaacgat tcccagcaga
    13501 aaaaaaacag aacctataaa atcatcatag gcctgcagct gattatcatt gcaattcttg
    13561 cagttcttct tttcaccacc aaatcaagtg ttgatacggt tgttcttgaa aaggaaaaat
    13621 cagttcagat gtccgaagaa ctacaggttg agcttgactc actgatggct gaacacgaca
    13681 aaatcaagaa agaatacggc tatctgagcg atcagcttac tactaaagac agtaccattc
    13741 tggcccaggc caaggaaatt gaaacgttga taaattcgca ggcagactac cgtcgaatca
    13801 agaaaaaact ggatgctctc agacagatta cgcagggtta tgtcaatcag attgacagtc
    13861 tgtaccgtgt gaaccaatcg ctcactgacg aaaacacaaa aatcaagggt gatctggaca
    13921 acgccaacaa aaaagcagat gagctcataa aggaaaaaga cgacctttcg accaaaatca
    13981 acgatgctgc ttacctgaaa ggatacaaca tcaatgcaac aggttacaat atcagatcgg
    14041 gatccaaaga atccgttaca gaccgcgcac gcaaagttga acttattcgt gtttgtctca
    14101 caattggtga aaatccactt gtgtcaccag gacaaaaaga cgtttatgtg cgtattgcac
    14161 gccccgacaa catcattctg actcaaggca gctattcatt catttataaa ggacaacgaa
    14221 ttcagtattc ggcaaaaaca gtcatcaact actctcagaa agcatcaaca atttgcattg
    14281 attatccgcg ggaaaacatt gaacttcctg ttggcaaata ccatatagca gtctttgctg
    14341 atgatcacga acttggccag tgttcaatca cactccggta aaatattttc aagctttaca
    14401 aagggctcaa tcttctggtt gggccttttt tattttaatc atgcatgatt tcataaggta
    14461 aatttttccg ttgctgaata gctcaaattt tagcacaatc tgaatctgtc ttcatcgcag
    14521 gcctgcctga ctgcctgttg cagttgaaat tgcagacagg tgaagtttga ttcgaacttg
    14581 tccgcccatc atgtcacgac agtgactgat gccggcgggt tagcgtcaat aagacctgct
    14641 gaggtaaatt agaatcttgg cgggaattcg agaaaccgaa gtgcctggaa ggcactgagc
    14701 tcatgaacac ctttgaaaat gatataatgg gtgaataatt cgcggacaag aagttggccc
    14761 agctgcggaa gttttcgggg atcgggtttt actcaatctt ccggttgggc tttaattaat
    14821 ttattgaccg cctgaaaaac cttcccggtt tcaaatcctt ttgaaatacc aaatcgtata
    14881 agtttttcac gcagtttcag cggctcatct ttctcaatgg ttttcagttt tttctgaagc
    14941 cagcccatca acaattcttc ctgatcacta acctgaaacg ctgaaatata gcggtcaatc
    15001 aacaaatcgg gaatactttt tttacggagc tccattttaa tttttaccgg gccccagccc
    15061 ttctgtctga ttttgctacg aacaaacaac tctgcaaatc tttcttcatt cacaaaacca
    15121 ttttgtatca gataattcaa taatgaatcc cagtattcac gggaaacatt catccgactc
    15181 agttttaatt tcacttccga aatgcaacgc tcctgaaaag cacaatactt ttcaagtttc
    15241 ggtataatct gattcatcag aaatccggga atattgcttt ccacaataaa aaattaaatg
    15301 gtaatctctt cgcctctggc attaaaaaca tgatactcca gcacatgata attgctggct
    15361 ggttctattt tcaatttgca gcggagtttc tttttccagg tctttgcaat atttttaaaa
    15421 aagcccttgg tgagatatgc ataaataaac ggagaagtgt ataaaaccag caccttttca
    15481 ttctgctcat tggcaaggaa agccaatgta ttttcaattt catcaataat gagcgaatga
    15541 gcttttatct gacccgtacc attgcaaacc ggacatttct ccagcacttc aacatgcgtt
    15601 tcaggcctta cgcgctgccg tgttatctgc accaacccaa atttactggg aggcagaatg
    15661 gtgtgcttgg ctctgtcgtg cgacatctct tcaacaagct tttcgtaaag cttccggcgg
    15721 ttagcaccct gatgcatatc gatgaagtca acaacgataa taccaccaag atcgcgcagc
    15781 cgaagctgac gtgcaacctc ctcggccatt tcaaggttcg ccgcaagcgc attctgttcc
    15841 tgatcctttc cgctatcggc cttgtaacca ctgttcacat caatcacatg aaaagcttcg
    15901 gtacgttcaa taatcagata cgcacccgat tttaatgtta ccttctgacc aaacaacgat
    15961 ttaatctgtt tatcaacgcc agttgcttca aaaacagacg ttttgccttt atgcaactta
    16021 acaatatcaa tctgatcagg tgcaatactt gacaggtaat tccgaagctc agagtacatc
    16081 gtattatcat tgacaacaat gctgttgaac gaagcattga gcaagtcgcg cataatggtg
    16141 aatgaacgat caagttcgcc gagaattttt accggaggtt tggcactgcg aagcgattgc
    16201 agcatgctgt tccagcgtcc gataagttca tccatatcgg cttttagctc gtcaaaactg
    16261 cgattttcgg ctactgttct gacaataatg ccaaaattaa ccggctgcaa actcgacgca
    16321 atttttttca gccgcgattt ttcattcaga cttttaatct tgtgactgat ggaaattctt
    16381 ttggaaaaag gcaccaaaac cagataacga ccaggcatgc taagctccgt cgtaattctg
    16441 gggcctttag acgaaatggg ttccttggca acctgaacaa gaatgctggc tccggaacta
    16501 atcacattgg taatttttcc ggtcttatca atgtcctgct ccagaggaaa agtttcaacc
    16561 gatacctgtt tgccggaaat aacctctttc tgatatttta tcagagtctt tatctgagga
    16621 ccaagatcca gataatgcaa aaaagcatct ttttcgtaac cgacatcaac aaaagcagca
    16681 tttaatcctg aaataatctt tttgattttt cccagataaa aatcaccgac accaaaatca
    16741 tgggtgccgc gttcggcatg caattctacc aggcgcttat tttcaataag agcaatctgc
    16801 acttctcccg aagcggcatc aatgattaat tccttgttca tacatgaaac tacttatgcg
    16861 gaatacccgc aaaagaaagc cggaagacta ttttctcttt ttgtgcctgt ttttacgcag
    16921 tcttttcttc cgtttgtgtg tcgccatttt gtgacgtttt ctcttctttc cacttggcat
    16981 agtgcagtat ttttaaatta tttttttcct ctttttacac tctccacaaa agtcttggca
    17041 ggcttaaagg cagggatatt atgagccggg atgatgattg aaactttctt ggaaatattt
    17101 cttcctgttt tctgcttgcg atgcttgata atgaaactgc cgaaacctct cagatatacg
    17161 ttatctccat tttccattgc attcttcaca atgtccataa aagtttcaac tgcactctgt
    17221 gcagtgattt tttcaattcc tgtccgattc gagatttcag caacaatgtc agcctttgtc
    17281 ataattatgt tgtttaaaat taacgcctta gtaggtgttt gggggtgcaa atatagaatt
    17341 ttttttgcac atgcaagttt tattttccgg gatttctcaa atttgcagtc atgaatccac
    17401 gccctgatat tagtcttttc aaacacctca taaattggta ccggcagaac aaacgaccgt
    17461 tgccatggcg aaaatatgcc gatccgtacc atatttacat atcggaaatc gtctttcagc
    17521 aaacaagaat tgatcagggt atcagctact ttaataatat tattgggaaa taccccgatt
    17581 ttatctcact gtcaaaagcg cacgaaaaag actttctcaa cgtctggcag ggactgggtt
    17641 actactccag agctcacaac attcttaaag ctgcttccat aattgccgaa aaatacgatg
    17701 gcgagctgcc ttccgaatac gacaaacttc tctcgctgcc cggaatcggg ccctacaccg
    17761 ccgcagcaat cagttccatt gcctttggcc tgcccattcc ggtggtcgat ggcaatgtga
    17821 aacgcgtaat cagccgcatc cgctgcatcg gaattcccac agacaagatc gagctgacca
    17881 acgaaattca ggatttcctc aatcagcata tccaccattt cgacccgtcc gatttcaatc
    17941 agtccctgat ggagctgggg gctctggtct gcactcctgc aaatccttcc tgcccagatt
    18001 gcccggttga aaaattctgc ggtgcattaa aatcaaacag tgtcgaaaaa tatccggtca
    18061 aagctgccaa aaaaacaaag ccggctgtgt ccattaactt ttacatcatt ttttgcaatg
    18121 ataaaaaaga tgctttctac atggttcggc gtgacagtga aaaaatatgg aagggtctgt
    18181 acgaatttcc ctgcattgaa acagatgagt ccgaagcatc ggtcaatagc acggaacagc
    18241 agtttccggc tggctttccc ctcgatcatg cacaacctgt cttccggaca aatcatgtgc
    18301 tgactcaccg tctcatcagg gcatcgtttt ttgagaccgt ttataaaaaa aaggttccgg
    18361 cacactggat aaaggttccg gtctccgaag ctcatacaat gccggtgcac cggcttattc
    18421 aggagtatct tgaacaaatg catgaccgga aaaaacaata accagctatt ttgtatcttt
    18481 gtaaaaaaag taagatgaaa agtatcaata aagttattct ggtcggccat gtgggcaaag
    18541 atcctcttgt aactgtattc gaaggcgggc gcaaaaaagc ttctttttcg ctggcaacca
    18601 ccgaaagata ccgcgacaaa accggtgccg accagtctgt aaccgaatgg cacaatatag
    18661 tcgtctgggg accacaggcc gaagtggttg aaaaatatgt ccgttccggt aatccactct
    18721 atatcgaagg ccgcattaaa tatcgcgaat acaccgataa ggatggcgtg aaaaaaagaa
    18781 tcaccgaaat ctatctggag aatttctcaa tgctcacatc ccgctctgca tcggatagcg
    18841 gcacagagat taccggcccc gaccatacgg ttcagccctc atcaaacgat ccccttagta
    18901 ctcctttgga caacgcaaac gatgaccttc ctttctgata tcggctcaaa gccggtgttc
    18961 tgaacttttc acctgaacaa tggaccctat ccttctttta atcattgcat ctctggtaat
    19021 tctcagcctc tttgcatttc tgagaattgc tgaatcggct ttcataagaa tctcctccga
    19081 atttctcgac gaaatcgaat cccctactcc atccgatcac cggactgatg actttctgga
    19141 taaaccggcc cccgttctgg tggctttttc cattatgaaa tattctttgc tgatactgct
    19201 gacaatagcc gttttcattg caacagagat ttgtcttcct cacacttcat ttttcgtcag
    19261 gtctttgtgt gttctggctg tcatctttgt tctgatctgg attttcggag aaattattcc
    19321 cggttttttc aatagctgca acgaacgaaa aactttccaa tcctgcttct tcattttgat
    19381 tccggcgaca gccattgttt ccatgctgaa aggagtgctt ggattttctt tcgcacgcca
    19441 cgaaagccgg attgcccgca aagacatgat ctccatcagc gatatttccg atgccatcga
    19501 aaattccgaa gtcgaagatg aggaaatcat ggaaaaacaa ctgattcagg gcattgttaa
    19561 attcggagat ctcgaagtaa aggaaatcat gcgggcccgc attgatgttg cttccatacg
    19621 cttcgacact acatttacgg atgtcatgga taaaatgctc gaagccggtt attcgcgcta
    19681 ccccgcttat ggcaacagtc tcgacgatat taaaggaatc atttacatca aagacctgct
    19741 gccggctttc cgcacaggtg aaaaagacta caactggcag cagcatatcc gccaggccat
    19801 gtttgttccc gaaaacatga aaatctcaca actactcgtt gattttcaaa cccacaaaat
    19861 gcacatggcg gttgttgttg acgaatatgg cggtaccagt ggcattgctt cgctcgaaga
    19921 tgtacttgaa gaaattgttg gtgaaataaa tgatgaacac gatgccgaca gcgaccagat
    19981 actggcccgg caaatagccg aaaacacctt tgttttcgat gccaaaatcc caattcacga
    20041 ccttacccgt atagcgggta tagacagcga cctgtttgat aaatttgaag gcgaagtgga
    20101 aacactgggc ggtattattt taagcatctg cggaaacttc ccttcaaaaa accagaatat
    20161 cacatacaaa aacattgaat ttaccgtttt atcgatgagt aaacaccgca tcggaaacgt
    20221 aaaagtgaaa ataaatgaat ccaatcagga atcttagcat cgtgttgttt gtggcttttg
    20281 ctgctgctat tgtctcttgc ggaggcggaa acgaaactta ctatccaaaa ccacgtgggt
    20341 tttatcgtct cgacctgccc gacaaaaaat atacgaactt cgattccgtt tatcctttct
    20401 cctgccgaat tccgcaatac gcgcatgtcg aatccatcga aagcaacgaa ccgggctcag
    20461 tctggttcaa tctgattttt cccgcataca atggttcggt taatttcagc tacaaaacaa
    20521 tcaacaacaa cctgtataaa ctgaccgaag atgcacgcga ttttgccaat aaacatattg
    20581 caaaggccaa tgaaataaaa gaaatacggg tatctcatgc cgaaaaccat gtttatggtc
    20641 ttatttacga tattgaggga accaactcag cttcgcctta tcaattttat ctgaccgaca
    20701 gtacaaagca ttacctgcgg ggtgcagtgt atttcaacca cattccaaac aacgactcca
    20761 tcgcacccat tattcaacgt gtgaaagaag atatcgacac gctgataggg agtctgaaat
    20821 ggaaaaatca atagccaaaa aaaagactgc cggtaaagac agtcttcagt ttttaaatcc
    20881 ggattattat tgaattccga gttttgcttt cacaaaaggc ataatgtcgt cagagtcctg
    20941 ataataaatc agcgcacccg ttccggtatc gaaaatgtaa ttataacctt tttcaacagc
    21001 aacttcatca atggctttct tggccttgtc aatcaatggc tgaacaagtt ctgattcttt
    21061 ctgagataaa agatcctgag cctgagcttt gaagtcatca atgcgtgtct gaagatcgag
    21121 caattctttc tgcttcatct gtttgatcgg ctcaagaaaa gtggcctcgt tggtcatata
    21181 atcctgatat tttgtctgaa actcattgtt cataagagtc agctgctcat caaggctttt
    21241 tgcaaattcc tgcagcactt tctgggctga atcacgaccg ggcatgactt ccatgaggtc
    21301 ggttgaattg atatggccaa gtttgagttt aacctgagcc tgaagcgact gaacatttac
    21361 gaacaggagc aatgagatga acaaaacaaa tacgtttttc ataaaatgga attttgtgca
    21421 aagttaaaag aattattcat ttgtattcag cccgcctgct ttatagccaa gcttggccaa
    21481 aacttcgtcg ctcttgtcgt acttttcatt cgcataaatg agcgtggtat tgccggcgcg
    21541 gtcaaaaata acagcaaatg ttccttcttc ggcaagttct tcaatggcat tgtacacttt
    21601 ttcctgaatg ggcttgatga gttcctgacg ttttttgtac aggtcgcctt cggttccgaa
    21661 atattttttc tgcagatcct tggcttcctt ttccttggca atgatttcat tctgccgctt
    21721 ggttttcatg tcttccgaga gcaaaaacga ctcggactga tatgatttgt acaacttgtc
    21781 gatttcggca aacttgacct caagctcctt ctgccattcc atgctcagct cgtccagtgt
    21841 gttctgggcg gtctgataat ccggaatgtt ttcaagtata tactgggtgt caacgtaagc
    21901 gaacttttgg gcactgacaa acatcgatgc aagtaacaga gccgcaaaaa taaagattct
    21961 ggttttcata gtgagttcct ccgactttta tttgaacgtt ttttttaatc gattgatgag
    22021 ttgattgaga aatggaattg tcccttccat gcgggatacc agggcacgtc gtcaaaaggt
    22081 atgccataat cgagacccag taagccaaac atcggcagat aaatcctgac tccaagtccg
    22141 gcactgcgtt tcacagcaaa aggatcgaat tcggtaaatt taagccaggt gttaccggct
    22201 tcgacaaacg aaagcagata aatggtcgcc atgggattgg ttgaaatggg atagcgcagc
    22261 tccatggtgt atttgctgta tatggttcct cctatcgact ggtttgatcc atcacgcggt
    22321 gtcagcaccg aatttgtgta accacgcata ccaatgattt cgcggccatc aagggcaaag
    22381 cccgaaagac cgtcaccgcc aagatagaaa cgttcgaaag gcgacaaccc gacttctttg
    22441 ttccacatgc ccaggaaacc gaatttagtt cttgacatca acaccatatc gccaaaaaga
    22501 cgagtgtata aggttgcatt gaatttccat ttgtgatatt ccagccattt gaatttctcc
    22561 tgatcagtgg ctgtagtata atccctgtcg ttgaaatagg aatagggcgg cgtaaaagtc
    22621 accgaagcag aaatctcgga cccggatttc ggaaaaatag gatcgtacga agaactgcga
    22681 ctcaatgcaa atccaaaatt cacattattg gcattccctg atgtgaatga atatgtctgg
    22741 aaatactcat caaaattgta gtactgataa gaaagcgtaa gatattccat gaaataatcg
    22801 tcgggccatt tcaatctttt gcctaaacca attgaagcac cggaaatatt catcacctga
    22861 cgttccagca gtgttccgct tgtactatag gttccatcgt cagcttttga aacaccgttt
    22921 gtctgaacgg aatggaaaag tgaaaagctg agggcattgg gtttcttgcc gcccagccac
    22981 ggttcggtga acgacatatt gtatgactga taatgggttc cattcgattg cccgcggatg
    23041 ctgagtttct gtccgtcacc ggttgggagc ggcctatagc tgtcgccatt gaaaatgttg
    23101 cggattgaaa aattattgaa agaaacgccg agtgttccca ccagacgtcc caaaccccat
    23161 ccggctgaaa gttcaaactg atcggttgaa gtttcctcca ccacatattc gatatccacg
    23221 gtgccatcag cgggattcgg ttttggatta acagccagct tttcgttgtt gaaataacgc
    23281 agctgggcaa gttcgcgctg tgttcgaata atatcggaac gattgaaaag ctgccccggc
    23341 tttgtcctga tctcgcgcag gatcacatgg tcgttggttc tcgtattgcc aacaagagtg
    23401 actttattga tggttgcctg tttgccttcg taaatctgta tttcgagatt gatggtgtcg
    23461 ccgacaacgc ttttctcaac gggtgtcacc gagaaaaaca aatagccatc atccatatac
    23521 agcgaagaaa tatcgcggcc gctcagatta taaaacagat tgctttcaag caaagactga
    23581 ttgtacacat cgccccgttt cactcctaaa atttcattca actgcttgtc ggagtatttg
    23641 ctattaccta cccatgtgat atcgccgaaa taatacttca atccttcttc gattttgatt
    23701 ctgagcgaca attccttttc attcaaaaca gaaaccgaat cgctgactat tgagatgtcg
    23761 cgataaccaa gctccttata ttttgcaata atggctgcct tatctttttc aaattcagaa
    23821 gggatgtatt tcgacgcctt gaataaatga taaatcttct tctgcttggt ttccttcatg
    23881 gatttacgaa tcaccttttc ttcaacgagg gtattgcctt caacagcaat ttcggcaatt
    23941 ttcattttct tgccccgatg aataataaaa tcgagggtca ccatatttcc ggcagtagta
    24001 tcgggttttt gggcaacttc gacctgggca ttgaagaaac ccttatcgac aaaatgagag
    24061 cggataagac gtttggcatt catcaatgta tgctgtgaaa ccacatcgcc acgaacaagt
    24121 ttcagttttt cacgcacatt gtctgcttcg cttttactga tacctgaaat gctgtatttt
    24181 gataaacggg gccgatcctg cagataaata tcaagataga taaaagtacc ctgcactgca
    24241 gtaaccgaaa taataacctg ttcaaacatg ccgtttgaat aaactttttc aacggcattg
    24301 ctgatttcat caccaggcac cttgatggat tgacctatgc gcaggccgga aacagagatg
    24361 acattgtcgg ggcttgcaaa aaactcaccg ctgacagtaa tgcccgcaat ttcatacgga
    24421 cgcgggtttc gataatcgac accgatgatt ttatcggttt gggcgcttac agcaacatgc
    24481 agtaaaacca gtatcagaca gcaccacaga gatttgaaca tgttcagtct tttttatctt
    24541 gattcgtaat aatttgctct cccgtcattc cataacgacg ttcacgtgac gaataggtta
    24601 caagtgcctt gttgaattct tcttcattga aatcgggcca ggtaacaggt gtaaaatgca
    24661 attcggtata ggccagctgc cacaacagaa aattcgaaat ccgctgctcg cccgaagtcc
    24721 gtataagcag gtcagggtca ggcatttcag cagtgtacag ataactttca aacagctttt
    24781 cgtcaatctg atcaggagaa ataatgttgt tggctacatc cttcgcaatg ttggatgttg
    24841 ccctgagaat ctcgtttctt gcgccataat tgagcgcaat gacaagtgta agcacttcgt
    24901 ttttcgaggt aaattcaatc gatttgcgaa gtgattcctg cgctttttcc gacaaccggc
    24961 tgatatcgcc aatggtcatc aaacgtacgc cattgcggtt cagttcttcc gtttcgcgct
    25021 ccagcataaa aacaagcaaa tccataatgg catccacttc ttctttagga cgtttccagt
    25081 tttcggtaga aaacgcataa agtgtcagat acttcacccc cgctttggca gcagctctga
    25141 caataatttt aactgtttca gctccctgcc tgtggccttc agtcctgtca agaccacgtg
    25201 ccttagccca acgcccattc ccatccatga taatggcaat atgctgagga atatcttgtt
    25261 gtaatggttt tgtcacaaaa gtctgattta aaaaatactg gattcgcttt tcataatgcg
    25321 cagatacttc gatttaaaac tatcacgacg cctgctacca ccaggacagg aatccgactt
    25381 atccttgatt ttaaaagtaa gcgtgagtcc tgcaaatgaa taccaatctt tggtggatga
    25441 attaccacgt tgaaatcctg ttttttcggt ttcagcactt tgtgcaggat ctgccaatgc
    25501 tgcagcaact tcggtgtttt gagccattaa tacagtggga tcggcgtacg ttgtgctcac
    25561 atcatcgata taatcggtaa aggttttacg tagtccccac tcaataccaa gaaccgttga
    25621 agacccgatg ccaaccttaa acccaagtcc aaacgggatg gcaaaaccag ccagattgta
    25681 tgggcttcgg tcgggatatg ccgtagtacc ctgtccttcg gttcccagtg actgaagatt
    25741 ataccagtca cctttgtaat aaccttgcgg gttgaacttg aaaaaagaag caccgacaaa
    25801 gaggtagggc gtaaaaaaat ccttatcgtg tccgatttta aatttgtaat aagacaactc
    25861 gtattgtccg ccaaactcca tcaagggcga gcgaaaacta agattacgat cttcgttgta
    25921 aagaatcacg ctgtcagcgg cctgaagaga accaaacaaa acatgcagac gcacagccat
    25981 ccgcggattc atattatacc gatacatgat gccaccggcc ggttgcgagg ttagaaaatg
    26041 gccataagga ttcaaatcgc cgatgtaata agaaacgcca cccatcaagc ccagctcgtg
    26101 ttgctgcgaa aagccggtga agcccgcaat aacaataaga aaagtaaaaa aaacacgtct
    26161 catttctgct ggaataacgt taatctgcag catttagtat ataaatgagc aacccattct
    26221 gaacaagaat gggcggctgc aaaattaggt aaaattatgc ttgactgttt gttcaaaatc
    26281 agaatttcgg gattgaaaaa ccgccacgcg gaattttgta atagaaagtg atttcggcaa
    26341 acatataggt atcatgatcg gtaggatcgc cccgctgcat attgggtgcc gtttggccag
    26401 ccacttcacc aagactggga tcggccataa aagcggcaat gtcacctttt tcatcacgaa
    26461 tttcatcatt attgaagtaa gtggtgctgc aatcatcgat ataatctgtc caggttttgc
    26521 gaataccata ttcaagacca atggaccatt ctcggctgat ggcatactta aatccaaacc
    26581 cgataggaat aacgggttgc acacgatgat agggcttgcg ggtttcaaca agaccctgcc
    26641 cttcggtact cagtggctta agtgcaatcc aatcaccatt gtagttgcct tggggattga
    26701 accacataag accaaatccc acaaaaatat aagattcgat attaatattt ctccaaccct
    26761 gaacaccgcg caacttgtag atatgacctt cgcgctggcg ggtaagcatc cactcaaact
    26821 ggccattgat ttcaataatc ggggtgcgga aattcagatt gcggttatga cggtacaatt
    26881 cttctgtttt tttatcatcg ccattcagat aggcaaaatg catcatacct ttgacacagt
    26941 gctgcttgcc cgttctgtag cgatagccca tactggtacc cggacgtgtt gcaggccagt
    27001 cgagatcgcg aaaaccattg gtgccaatct gattggcgcc accaagatcg cccagaaagt
    27061 tggaagcacc caaaccaaag acgtattctt ttcgttcacg gcgccattgc tgggctgaag
    27121 ctgtatcaac tatcagaaat gccaataaaa ataatatggg ccaggtgatt ttcataacga
    27181 atagtttaat cccaattaag tggcaaagat aataaaaaaa cggttgaaaa taagaaaata
    27241 tttctttgaa ttaaaaaaac ttatttattg cgcatatctt caccccacat cagtttttca
    27301 cggatcacac caaaaaagct gtcggcaggc ctgcgtacaa agttgagggt aaaaggcgcc
    27361 ttgcgtattg ttaccttttg accccgtcca ataacaatct gactcgaatc aatggaaaga
    27421 ataaaatctt cggttcggcc atccggaaca aaagtgatta catctttatc agaaatcaca
    27481 agaggacgaa ctgacagatt atgaggtgca ataggcgtta aaatcagatt ttcggcttcc
    27541 ggaacaagaa tgggacctcc gcaactgagc gaataggccg ttgagccggt tggggttgcc
    27601 agaataatac catcggacca ataactattc ataaatgagc catttatcca tgtgttaatg
    27661 gaaatcatcg aactggcact gcttttatga acagatacct cattcaatgc aaatattttt
    27721 tttccaaata aagattcaca atcatcaatt tcgagcacag tgcgtgcaga taaagtataa
    27781 ttgcctttga tgaattcatt caatgctttt cgaaactcat ccttacggat atttgaaaga
    27841 aaaccaagtc tgccaaaatg aacccccatt accgggactt cactttcgcg gatcaggttg
    27901 accgtttcga gcaaagtccc atcaccgccc atgctgacaa caagatctgc tattccgccc
    27961 actgtttctc cttcggttaa aaaacgggta ttgggaggta atgaaaaagc gctgctgatc
    28021 tgtgcttgca aatgaccaca aaaaatggcc tgaccccctg tttgtaatac aacatcaagc
    28081 acagcctgaa tttcatcatt gaaatcaggg gagtaacttc ttgaaaaaac acaaatccgc
    28141 atcctgaaaa tattttatac cggtgctgtg aaatgcaaaa atataccttt ttcacgaaac
    28201 cggttcatgg taaattcgac tctgaatact ttgtcataat atgttaccag atctattccc
    28261 agcccaaaag cagcaagata atcgcccgac agattattgg cacccgaaaa caaaccccga
    28321 tccgaccaac cctgatcgaa aaaagcattg acaaaaacag aaacaggaac acgattaaac
    28381 ttgctggttc tgacaaaaag aattttaaat attctttgct ggagtatatt atatttcaag
    28441 ttattgcgtt gaatgaaaaa atgttttacc ggtataaccc ggtattgata gcccctgaca
    28501 aaatcatttc catagcccat aaatccagca gttgcaaaat tgttccctcc agccagtgaa
    28561 acatacatgg ttgctccggt agcaaaatat acagggccag ccagattaaa atatttgcgt
    28621 gaagtgcttt tcagataaaa ctttttgtca tttttttcag aaaaacctgc aatattaccg
    28681 agattcaact ccgcatcaac ataccaacct tttaaaggat aattcttcac gtcacgaaaa
    28741 tcatacctgt actgataatt caacgtaaga cgccgccata agtcagccgg tgcataatca
    28801 ggattgagct ggaataaaga atcggcatat ctggttgatt caaataacaa agaaatctta
    28861 tgcaattgat acatgccggc tctgcgcgtg gttgcaacag aagtaaacaa cccggaataa
    28921 acatattcat cgggaagttt caatgtcttt ggctcgtgat tcaaaacaag atatgacaaa
    28981 gaatgagatg cctcgaaccc caatgctgct gaaattcccc aattctggtt tttatcaatg
    29041 tacggttttg aatacgatac gccataaatc tgattgtatc ctgttttaaa caatagtttc
    29101 aggtattctt ttcttcctct gaagttttcg tgataaacat acaaaccata ggttgccttt
    29161 tcaaatgaca tatcgcgcaa ccatacattg aaatttcttt cttcgatatt caaaaatggc
    29221 atgggccata tataccagcg ttcagtaacc cccacacaaa catttccact gtcgctgaaa
    29281 acaactgaag caaaatgaaa aagcgatgta tttacaagat tttgttctgc ccgcaaagcc
    29341 aattctgctg tatcactcat caaaacagta tcacctatgc caaaaggcat ttcgcgcaga
    29401 atcacacttt cctttgttac accatttccg gcaatcgaaa tatgtttaat gaaatataat
    29461 gaatcagctg cacttacaga aaatacaaca gagattaaaa atataagaaa gaaaaaacgg
    29521 atattcaaaa tactacatat tgaggtatcg catcagcaat tcatatcggt cctgcagaat
    29581 atcatcaatc atatcttcgc caaaagaagc aacaatatta tattcgtaac gggtgaacgt
    29641 ttgaaggatt tttccgatat cggaattgtt cactttcaga acaacctgca tgcgagtgct
    29701 gtttggaatg gtttgcacaa acgatgcaat aattagagcg tcatttgatt caacaatatt
    29761 tgatatctga gtcaaggaat aatctatcac attgagttca ataataataa ttccgccggg
    29821 attattcaca gaaagcaact cagctaccac ccgcaaaacc gaatcggaca aaattacggc
    29881 agacagcttg ttgtttttgc cggtaactgg aaccagactg agtttatgct gatagatggt
    29941 gcgcagaatg tcgaaaatat gagcattttc atcaaccgaa gcgccctgaa tcatcagttt
    30001 tatgttgcca atgggctgcg aaaaatcatt caagccaagc aaatcgtctt cgcagaccag
    30061 accaagatac tcttcattat tcacaatagc caggtgtgta attttatatt cttccatcag
    30121 cgataatgct tcggaagcag cagtactggt tttaacactg ggaatggcag aaggggttat
    30181 atcggcagca aaaagactca tcagaaaaag tatatgcgac aaaaatataa tttttcaggc
    30241 agcaaagtat taaatttgca aaaaaaacct gatatgacac gtctcagtgt aaatataaac
    30301 aaaatagcaa cgctgcgaaa tgcacgtggt ggcaatgcac ctgatgtact taaagcagcc
    30361 gttgattgtc aacgcttcgg agcacaagga attacagtac atccgagacc cgacgaacgt
    30421 catatccgct atgccgatgt ttatgcacta aaaccactag taacaactga attcaacatt
    30481 gagggctatc cctccgacaa tttcataaaa cttgttcttg acgttgttcc caaccaggta
    30541 acattggttc ccgatccacc cgaagcactt acatccaatg ccgggtggcg cgtaacagaa
    30601 aacatggctt ttctgaaaaa agtgattgaa gactttcatt ctaaaggaat ccgggtttcc
    30661 atctttattg ataccgatac tcaaaacatt gcagccgcag ctgacaccgg caccgaccgg
    30721 gttgaacttt atacagaacc atacgccact cattttcctc atgaccgtca gcttgccgta
    30781 gctgaatttt caaatgcggc tcgtgctgcc atcaaagccg gtctgggact caatgccgga
    30841 catgatctga atctcgacaa cctccgctat tttgccgaaa acgtccccgg tcttcacgaa
    30901 gtttctatcg gtcacgcact catcagcgac gctttatact tcggactgga aaataccatt
    30961 cagatgtatc tgcgtcaatt gcagccagca tgaaattatt ttttcgcgaa tacggtcagg
    31021 ggcaacccct cgtaattctg cacgggcttc tgggcatgag cgatcattgg attccgctgg
    31081 caaaacaatt tgcagaacaa tttcatgttg ttgttcctga tttgcgcaat cacggccaat
    31141 caccgcatgc tgaaaaattt tcatacaaac acatgcaggc cgatattgaa gaacttctga
    31201 gcgatcttca gataaaatca tgcaatctca ttggtcacag catgggcgga aaactggcca
    31261 tgcatctggc tttgaaccac tccgaaatgg tggacaaact tattgttgcc gacatcagcc
    31321 ctattgatta tccaatcgac gaagacaatg aaatggtgca ggccatcaac agaatgaatc
    31381 tttcatcggt aaataaccgc gaagaacttc gtcaaatgat acaatgggca acaaacgacc
    31441 ccatgctgca gggattgatt ctgaaaaaca tttcttcaaa aggcctttct gggttttcat
    31501 ggaaaccgga tctggaaaac atcacaaaga atctgcagga gatttatgat tttcgctgtc
    31561 acgaatttca gatccgcgcc agtgaaagca ttgataaagc cagtttcaac aaagaaacat
    31621 tgtttttaaa aggtgacaac agtcaatata tttcggaaaa tcattatccg aaaatcgcag
    31681 cattgttccc gcaaaataaa ataacaacca ttgaaaacgc aggccattgg ctacatgcag
    31741 agaagcctgc ggagttttat gaggtctgca tgaactttct cctttgaaat gtatcccttc
    31801 gatgggtttg aaccccttcg agagatttta tcacgtcctc accgcaaact gacaaaaaca
    31861 aacctccggc ggattaaaaa tggacttttt ctttttgaac atgtaaattg tcacgttaat
    31921 tttgcggaag tttttgtatt tttccccagt tttttaacat tgtcatttta taaaattgtt
    31981 gtcatgatta aacttttacc cgccttactg atacttatta caatgctgtt ttccaatacg
    32041 gatgcctact gcagcaataa attcatcaat caaccgggat cttattccgg caaaacccaa
    32101 tatcttaaaa ctgttgcagc cggctgtttg cctgcatcgg catcggccga gctgaacatc
    32161 ggcggcgtgc gtgcccgcat caacaatggc ggagacatgt ggtgggatct ggctgactta
    32221 gctgaatacg aggttccaaa aggcagcgga agaacatcaa tgtttgccgg agcattctgg
    32281 ataagcggac aagacattaa caacaacatc aaagtggcgg ctcatcgtta cagggcaaat
    32341 ggcaatgact tctggtcagg ccctctcacc atcgacggga cagcatctac cgaagccgcc
    32401 atctgtcagg attacaattt catcagcacc atctatcggc aggatgttac cgactttgtg
    32461 aattattata acaacccggc tgcctatccc ggttacaata ttccctcata tttctacacc
    32521 tatcctgcac atggtgatcc gtcacttggc cattcctttt atctcgctcc cttttatgac
    32581 aacgacgatg atggcgttta cgatccccaa agtggcgatt atccgcttta cgatatcaac
    32641 aacagatact gtcatcaacc agatccttca gcagaagata tgaatgggct tacgaatggt
    32701 ggcgttcttt tcgaccagat gctgaaaggc gatcagactt tgtggtgggt gttcaatgac
    32761 aagggaaata cccataccga aacgcaggga acatccattg gcatcgaagt gagagcacag
    32821 gcatttgcat tttcaacaat tgatgaagtg aacaacatga ccttctattc attcgaagtc
    32881 attaaccgat caactgaaac attatacgag acgtatcttg ggtttttcac tgaccccgat
    32941 aatggttacg gcaacgatga tttaattgga tgcgatgtgc ctcgcgggct tggctattgc
    33001 tacaatggtg atgattacga tgaaagcgga aatggcgcat atggctatgg gagccagccg
    33061 gcagcagtag gcgttgatat tctgaaaggg ccctacatgg atgctgacgg aattgataac
    33121 ggggcctata ttcagcatgt tgattcactg ggaaacctct ggtttgaaaa ctgcaatgcg
    33181 gctgttaatg gcagcaattt cggaaacgga atcattgatg acgaatgtta tggattgaca
    33241 aattttttga atttcagagg aagtggcgca atacccccgt atggcacaga ccctgatgtg
    33301 gcagcagagt atttcaattt tatgcgcagc atctggaaag acaacacgca catgatttat
    33361 ggcggaaacg gtcacataat aagcggtgga tacggccctg aatgcaattt tatgtttccg
    33421 ggtgaaagcg atgtctgcga ttacggaacc ggaggaatcc ctcctaatgg tcccaaaaac
    33481 tggacagaag aaacagccgg aaatcagcca tatgacaggc gcattgttca atcatccggg
    33541 cctttcacca tgcacccggg cgaagtgaat tacatgacca ttgggattcc gtgggccaga
    33601 gccaattctg gcggagctgc agaagcgctt gaacttttaa aatgcgcaga tgacaaatgc
    33661 caggcgcttt tcgacaattg cttccagctg ctcgatggtc ccgatgcgcc cgatataatg
    33721 gttcaggaac tcgatcgtga agtaatttta tatctcggaa attcaaattt cagcaataat
    33781 tatcaggatg catacgcaga agctgacccg accatcgtag caccatcggg ctacaactat
    33841 gattcactct accgtttcga agggtatcag gtttttcagg taatcgatcc gaatgttact
    33901 gctgccgaca ttgctgatcc cgacaaggcc agattggtag cccaatgcga tgttgaaaac
    33961 ggagccgcca cgctggtgaa ttacgtttat tcgcaacagg tcgggaccta tgtcccgcaa
    34021 atcgaggtgg aaggcgccaa tgaaggaatc gttcattctt tccgcatcat cgaagatatg
    34081 tttgcaagcg gtcaaaacac ccatctggtc aatcataaaa aatattatta catggtgctg
    34141 gcctacggat acaacgagta tgaaaaatac agctcagatc cggcgatgca gttccccgga
    34201 accatttcat ccaacggtca gaaaaggcca tatcttgcag gccggaaaaa catcagtgtt
    34261 tacacagcaa taccgcatgc gccagctccc gaatcgggcg ggacactcat caacagcgca
    34321 tacggcgcac agcccgttat cacacgcatt gaaggacagg gcaacggcgg gaacatgctt
    34381 gaactcaatg ccgcatccag aaataaaatc ctcgaagatg gcttttccgc tgagctcacc
    34441 tacgaagccg gatttggccc tgtgaccgtg aaagtgattg atccactcaa tgtaagagaa
    34501 gccaattaca ttctgaaact ggactcatca agcgcagcat gggcaggaga aatcaattca
    34561 tgcaaatggg tattggaaga atatgacatg agcaatcatt ttgttgcagc atattcgtcc
    34621 gaccatgaca tcagcagtcc gtttgaacag attttcacca atcttggaat tgccgtttgt
    34681 tttgtcgatg gaaaaaatcc aggagacctt acacatgaaa gcaatggatt gatttactcc
    34741 gacatcatat attcaaaccc atcaatacaa tggctgggag gtctttccga tgccgacggc
    34801 tcatcagcat ttaattggat acgaagcgga acacaggatt cagaaaacaa catgtataac
    34861 gactttatat attccggcca atatatggat ccggaagagt attatgaaaa aataatcgga
    34921 ggtagctggg cgccataccg ccttgcatca tacgaaaatg acaatccggc attaaaatcc
    34981 attgcgacaa gtatccaaac aacaaacaag atgtcgaatc tggcaagtgt tgatattgtc
    35041 ctcacgcccg ataaaagcaa atggtcgcgt tgtccggtac ttgaaacatg cgatgatcca
    35101 ttgctggcac agggaggagc cggaaaactc acgctgcgcc atcatgcatc ggtggataaa
    35161 aacggagaca cactcacgac tgaggcaatc tatgacggta atgaacaagg catgggctgg
    35221 tttcccggat atgccatcaa tttggaaacg ggtgaacgac tgaacattgc ttttggagaa
    35281 gacagctggc tgggcagcga aaatgggcga gacatgctgt tcaatccgac atcggatctt
    35341 gaaacgacgc tcggtcagcc acttttcgga gggaaacact ttgtttatgt atttgggcat
    35401 ctgagcgaaa gcgccaacga ctgccccgcc tacgatgagg gaaaatggct ctatgacatg
    35461 ttttcacaag aaacaattac agcgttgcgc tatattttca ccagcgccat gtggtgttcc
    35521 attccgctct caatcgatgg agagcaatgg ttgaataatg aatgccacat ccgtatccga
    35581 gtatcaaaag catataataa aaactattct acattcggat ctgcagcacc acaaaacaac
    35641 aattacccaa tgtacagctt caacacgtca acactgatga ctgtaaccaa tgatcaaaca
    35701 acagctatga atgcacttga catgattaat gtagtgccca acccatacta cgccgttgat
    35761 gattatgaag agaacatcta cgacaataaa ataaaaatca cgaatgtgcc gtcgaaatgc
    35821 acaatcagca ttttcaatct cagtggtacg ctggtaagaa aattcaaaaa cgataatccc
    35881 catgtaacag acattgactg ggatctgcgg aattcagccg ggaagctggt ttcaggtggc
    35941 gtttacatca ttcatgtgta tgcaccggga atcggagaac gaagcatacg ctggttcgga
    36001 tcgatgaaaa ccgttgtgga aaatgagttt taaaaatacg agatacgaaa cacaaaatca
    36061 gaaatacgaa ataaaatgtg gaaatgtgcg gctcacgtga agcgtgacag gttcgcgcat
    36121 gtgaaaatat gagcgactaa aaacactttt gcgatgatgc gatgggtgcg atgggttcac
    36181 tgtcacggat aacatatccg cgccagtgga tgaaaatgca caaaagaaaa caattcagct
    36241 tcgactcaga gcaccgacgc ttcggtcggc acatattgct gattgctgat acctgaaagc
    36301 tgattgcttc aaggtaacga ttcccgcttt cagcgtgata aattccacgt ctgtaacgat
    36361 tgcaacgaaa gaaggcattt aaacacgatt ataaatctga tctctgactt tttttgtaag
    36421 ccctttaaag accaaatcat aactaccatc aatcaattgc atcaaaagtt tttcatcgaa
    36481 agaagttgct tcgttcacat caatccaatg agttttactc atgtgataag ccggagttat
    36541 ggcggaatag cgctcctgaa gctctattga atactccgga tcacatttca gagcaattat
    36601 aaagacgcct tcaatatctg taagtgcaaa cattttcccg ccgactttaa agacaagagt
    36661 tgtttcatcg aaaggaaatt cctcggtaac accgggcttc aacaagcagt attcgcgaaa
    36721 actttcaata ttcatttatt tggcaatcca atattccgga ttttgcggaa ctttttcttt
    36781 ccatatctca aaatgcagta cagtagcacc ttcttcatca actgcgattg gcccaagatt
    36841 ttgcttcgaa gaaatactgc tgcctttttt tactgcagca gttttaatgt ttgaataaac
    36901 agacaggtat tctccatgac ggatcataat catggttgta ccattgggag cagtaataac
    36961 agcacttact gttccttcgt aaacagcgcg ggcactactg ccttttgggg cactaatatc
    37021 aattccgttg ttttttacct taatgtcgtg caacacagga tgcggatgtt ctccaaacct
    37081 atcggtaata gcgccctgcg caacgggcca tggaattttc cctttgttac ctgcaaaagt
    37141 gtttgaaata cgagcttctt caggtgtcat ctggtatgca ttgggcttag aattacttgt
    37201 tttattgttg gctttcaccg tattggcctt cttcttgctg gcttcgattt cagcttcaat
    37261 cattttttga attttgcgct gaatttcggt cgcttcccgt tgcttcttct tcagattggc
    37321 ccgcaatttt gattcttgtt ttttcagatc ctttacaact ttatccttct cttttttctg
    37381 cgaagcaagt tggttttttt gtttttcctg atccgattta agacttagtt tttcattgcg
    37441 cattccttca agttcggaca gcgacaactc aatatcagag ctggtttgcg aaattctttt
    37501 cacctggttt ttcctgatag ccgaatactc cctgaaataa ttcatccgtc gccaggccat
    37561 cataacactg cgagagctaa aaagataaac aagtctgttg gccggtttac gggtaaggta
    37621 tgcaaaatat accattcgtg catactgtga tttcaatgta ttcaattctc gcttcagttt
    37681 ggcaacagtc aacttctttc tttctatttc tcgctccaac aaagccattt cggcacctgt
    37741 cttcgaaata agactttcgt aattccgcac ctggctttcg ataagtttaa tctgttgagt
    37801 cgtctttctg ctgtttttct gtgtttcctt aatcagcttt gaggtgttgg agatttcttt
    37861 ttcaatattc gctttctggt tctgaagctg tttcttttta tttgactgcg cgcaaagatt
    37921 gttcatgccg aacacagcaa aaactgcata aataaaaatt aaccgcaaaa ataccgaacc
    37981 atatttcatc acttattttt ttgttgctga tccattggaa caaaactctc agggatgaca
    38041 aaaggaacca ttatttcctc atcaacacta actttttcaa aatcaatgac gaccttcatt
    38101 ttctgttcgg cttcgacatt tacatctatc tgatgcgcca gcaactgatc atcgaaaacc
    38161 acaaaagcag aataatcaac aaccaatttg ctgttttcct ttgaaatctc ctttatttgt
    38221 tgtctgataa ttttataatt ttcgggcgag atccacaaat cctgcaccaa tacacgatcc
    38281 atatcgctct ggttggccac atagtttttc agtttcttcc gccccggcgt tgatatcttg
    38341 taggacctgg catcttcacc cactttgaat atgttgtttt cgtaatagga aaaatcgttt
    38401 ccggtaacca atgcctgtaa catatcaaaa tcgaaaggtg ttttaagcaa ttcattgatc
    38461 agctgataat cgcctttaaa atatgtttta ttaagtcggt taatcatttt tacactgtcg
    38521 ggtgtaataa atatccggaa cgcctctatt ccaaccatgg ccgataacga caaccatatt
    38581 gtcctgtcct tgataatacg catgctgccg ccaaacccaa gattgttttt ttcagattcc
    38641 actttggcct gaaacttcac attcatcgtg cgaaaatcga actgggcatc cttcattttc
    38701 gaaaaaataa gttcaactcc agtatcctgc ttcacagttt cctttatttc cttttttgct
    38761 tcacgcccgg ttttacaagc ggatgtaaac gcaattgcaa ttaacagtac cggcaaaaga
    38821 aatttactca taatattttt tgtcagtaat tttttgatca atcatcttac ctgcaccccc
    38881 caattcccgc gccatcagcc aaaagcgtaa ggcatcttct gttttattct gaaaaaacaa
    38941 cacatcggca tactgttcca gcaaagcacc atcatcatcg cctccaagtt caatcgcttt
    39001 acgatacgct tctgccgctt ccagatactt tcccaaacga aacaaaacaa gtccatatgt
    39061 atgctgaaat tcagctttat cttcgtttcc cgtaagtgct tcagcaatca gctgctgtgc
    39121 cttgtcaaca tttttctttc gcaacgccag cgagtaagca tatgcatttt tcgcaaccac
    39181 attgtccgga tcttcaccaa tgagtttttc aaacatggca tcagaatcgt cattgcgtcc
    39241 ggtttttgcg taagtgtcgg ccaacagaaa caaagattcg tttttcattt ccttatttga
    39301 caaacgaaga tccgaggcaa actcgagaga aacgacggcc ttttcaaaca tagcagcatg
    39361 atattgagcc aatccaagaa aaaaccagac agagctttgt tccgggaaca attcagcggc
    39421 ctcggaagca tatgtcaata acgaatcata ctgctgatta tcgtaaaaca cattcatcat
    39481 cagattccag atggtaaaaa cacttttatc aaattccagc gattttttcc acatgtccat
    39541 ggcttcatcc atcctgtcat cagcgttcaa aaaatcggca tacattgccc aggccttggc
    39601 ttcatcggca tgaacactaa caagcgtatc aagcatcgga taaactaccg tcgtgtcgcc
    39661 atacacattg ccagccctat atacattcat aagcagactg attttctcat caattccagt
    39721 agataccgat ccgataatat aaaaaatctc cttcactgca agcgaatcgt taccagacat
    39781 atgatagtaa tctgccagat aggcatgagc aagtgcattt tcaggatcaa cagtcaaaac
    39841 atcattgata ctatttaaag caagccccgc ccgtccagtg cgcatataat attcaaccag
    39901 agccatagaa tactgcggat tttcgggaaa atgatcagca agtttctgaa cttcattcat
    39961 ggccttatca gtctttgacc aattctgata aatataatac ttttgaattc ctatctgctc
    40021 tgttatacca gtctgcaact cgatttcatt gtaaacatca atggccttct gaaattcatt
    40081 attctgaaca tagcactggg cctcatattc atacaattca tatttttcag gatacttgtt
    40141 gcggagattt ttatactgaa tcgccgcttc actgtatttt cccgattttt caaatgcccc
    40201 tgcataggcc agtttatacc agatattgtc aggatccaca cgtatagccg ttttcagaag
    40261 atcaacggct atatctgttt caccgttaag cattttcaag cgggccaatt catacaatga
    40321 tgcatcgtgg agtggatcct gctcaataat tgtttgatac aaacccatgg cattggcagc
    40381 atcgcccaaa attttgtatc gtgtggcttc aataaaagca ctgcttatca tcagcagttc
    40441 acgatcactg agcttgttgt gtttgcgctg tgccttatga aaagaattac atgccgttga
    40501 aaacaacagc aatgtaagca gatatatagc cagcacttta ctcattgaac aatcacagta
    40561 aaatcaccaa tattaagttc cttgtatttc tcactatatt caacgaaatt acccaccata
    40621 ctattgtcaa tattggcatt tgaaattttg caatgcgact gaataatcga atttgaaatg
    40681 atgcagttgg aaatcaccga tccttctccg attgaaacgt gaggtccgac aatgcaacca
    40741 gttattgtta catttttgcc tatatagcaa ggttgaatga taatggaatt tttctggttc
    40801 agattgttgg caattaaact gccgtttttt gcaaaatcaa gaatgcgtcg gtttgtatga
    40861 atggttgcat tcttattacc gcaatcaagc cattcttcaa cattataaac agaaaagctg
    40921 catccgcgct gcatcatatt ttcgagcaca tcggtcaact gatattcgcc attaacaagt
    40981 acttttgcag caagcagttt ttcaatttcc gccctcagca cttccccttc acgaaaataa
    41041 tacaccccaa caatggcctg atttgacaca ggtgaagttg gtttttcaat aaattttgta
    41101 acgatgtcct tgttgtcagt cttcactact ccaaatgctg aaggatcttc caccttatga
    41161 acccagataa ttccttcgcg agaagtatcc attttttgtg ttgtccgaaa caatgtatcg
    41221 gcaaatgcaa cgataacctt tccagagatg gattcggctg cacaagagat ggcatgcgca
    41281 gttccaagtg cctgattctg atagtatatt ttccctttag atccaacgct atctgcaatt
    41341 ccaagcaaat gtgcttctac ttctgcacca aaatccccaa tgatgtatgc aatttcttca
    41401 actttttcat caaccaattc catcaaatct tcaacaaggc gctgtacaat gggtttaccg
    41461 gcaatattta tgagcggttt gggaatggtc aaagtatgcg gtctcatacg cttacccata
    41521 cctgccattg gaataataat tttcataata atctttttga attattgttt acctgtatgt
    41581 ccaaatccac cggcacctct ctgagttgaa tcaagctctg aaaccagctt ccattcggca
    41641 cgttcgtgtt gtgcaataat catttgcgca atacgatcac cattttttat tacaaaatca
    41701 gtatcagaaa aattaatcag aatcacttta atttcaccac gataatcgct gtcgatggtg
    41761 ccaggtgtat tcaaaacagt aacaccgctt ttaatggcca acccactgcg cggacgaacc
    41821 tgtgcctcaa atccggccgg gagtgatata aaaagacccg taggcaccag cttgcgttcc
    41881 attggtttta aaacaatttc atcatccaag aaggcgcgca aatcaactcc ggccgaatat
    41941 tcagtttcgt aggccggcaa tggattattg cttgtgttaa caatattgat tttcatcctg
    42001 agcttatata aaggtgcaaa tgtaaacatt tttgcgattc cgggaaagtc agtatgaatt
    42061 caaccttcga agaattaaga actcttcgaa ggttgcaaga acacgttgaa aaaaacaatt
    42121 ttatttgaaa gcgctttcgc ttagtccggg gccgctcagg gcaagctttt cgaaatactc
    42181 cggaactggc ttgccattga gtttgtcaac atacactttt gccagatact gactcagcat
    42241 aaaaccatga ccacgcaaac caacaaataa tccaagcgat ggatcgacga tatagcgtgg
    42301 ttctacgtaa taaccactcc acacggcctg aaatccggta gcagataatt gcggaatcca
    42361 atcacagaaa atttcactca ctatttcaag aaattcctta ctgttgattt taaggttttt
    42421 atcagtctct ttcgggtctg tgagtggcga agcgcaaccg ataatctgac cggtttcctt
    42481 cagttgctgt ccatacaccg agctgaacga tttgtatttc ctacgatcga taagcatatc
    42541 aagagcatct ccatctttgc ccatcaatgg cagtctgcgg gtaataaatg cctgatgacg
    42601 cacaggatac aaccctgttt cgatattcag tttacgggca aatttgtcgg ctgttggtcc
    42661 aagagcattt ataaaaactt ctgtttcaaa ttcaatatat tgtttgttgt gatcgcgtac
    42721 caacacaata taacttgcgt cttccctacg aagatcaatc agttcacaat cttccataac
    42781 agttccacct ttctcaatac caatgctgcg tacaagatca ataacgcggc caggagtggc
    42841 ttgccagcaa tcatgtgtaa tcagggcaga ttgataagtc tccaggtttt tattgaaata
    42901 cgggctgatt tctttttgga aatccttttt atcgaccata taggcatcgg accaggcacg
    42961 tgaggcatcg agggccttaa acgttgcgtc atcgtgagca aaactcacat aacgtattgg
    43021 tttgtaatca acatttttga ttttctgcag ttcaacaaaa atatcacggt tatgaatagc
    43081 aatatctgaa agctccggaa cgctgaatgc tggccgtcca ccagcaatgt ttcgccacga
    43141 agcaccgcgg tcgtaattta ccagaacagg actgaatccg gcctcggcaa gataacggaa
    43201 cagagcgctg ccaccgattc caccaccggc aacgagagat ttcaccttgc gacgttcggt
    43261 ctttttaccg ggagtaaaag gaacgataag ttcgttttcc ttgccgggat acaactcgcc
    43321 gatcagcacc tgatttgaca atgggccacg tggagtagca tcgccaacaa cagtaatacc
    43381 tttcgacgcg atggttgatt tcaatcgtcg gatgcagcgt ttaccacggc aggcgcccat
    43441 tccaagacga gtagtgtgtt taatctcatc aattgagata aatttccggt cacccactct
    43501 gtccattatc tcatcaagag ttacatcatc acaatggcaa acataggtgg ccccttcttc
    43561 tttataactt gttgtctgaa gttcaggttt ttcaggataa ttacttttaa caataaagcc
    43621 acgaacctga atgatatctt cgccatgaag cgattcagcc ctgacccgtg caacattggt
    43681 tttgtttggt tttttgagga ttttttcaat cacaccttca ccaagaactt ttccattgtt
    43741 atcaaccagg aatacggctt cattttcatt tgcttcgtat tcaatcggaa gaaagagctg
    43801 atctttttcc gtattatagc caaaaatggc cagtccaggg cactgataca cacactgcat
    43861 gcagccgatg catttatcaa aatcaacaac gggaaccgta ctggttgaag tttttgtgat
    43921 agctccgtgt ggacaagcaa aagtacatgg attacaagca aaaccataca aacaatcgat
    43981 ttgaacaaat ggtttctttc gcctttcgcc cacaggcaat gccgattctt caattacgcg
    44041 caccggatgt tgctgcgaat cgatatattc ttttgaaaca gccaggtaat catcatattt
    44101 atacttcacc gacatcatat caagcacctc gtaagcagct tgttttccac gaagcacagc
    44161 agatgttccc tcccctattc gaatggcatc accgacacca tgacaattcc gtccgaaaat
    44221 ttcattgcca agaatcaaca actgatcgtc gggaacaagt cctgtacaga tattaatggc
    44281 atcaataccc tcaataattc tttcagtccc gggaacaggt ttaaaatttt cagcttcggc
    44341 aataacagca ccaacaatac ctgtatggtc tttgtttgga atagcgctta caagaatatg
    44401 attaagcatg ataggaatgc caagacgtcg gacacgattg gcctgaaccg gaaatccacc
    44461 ttctttaccc tgagcttcta tgattgcaac gacctgagca cctgcctgca ttaactgata
    44521 agaagtaaga tatccgatat taccagcacc taccgtaaga atttttttac caaggagcgt
    44581 aaattcagta ttcatcattt tctgcaccac agcagcagtg taaacacccg gtacatcatc
    44641 attttcgaat gtaggcataa acggaactgc accacttgca acaatcagat gattacattc
    44701 gatgtagaaa atctcttcgg ttttaatatt tttaactgca atgcgtttgc cttcgagcaa
    44761 atcccaaacc gtgcaattga gaaaaatacc ttcatgattg tcgcctgcca gcgccgaagc
    44821 aatttcaaaa ccacgcatcc caccatattt cttttccttt tcgaaaaaga aaaactgatg
    44881 ggtttgcata ttaaactgtc cgccaatttt cgcattatta tcaataacga tatttgaaac
    44941 gccatgctga ttgagtatct cacgtgaggc aagtcctgca ggtccggcgc caacaattac
    45001 aacatcggtt ctatatactt tggtttgcgt ttgttcattt cgcttctcat ctggcatgta
    45061 atcttttgga acttctttca cttccttcac gccatcgaca agtgtgatac agatacgacg
    45121 tataacgcca tcaacaagca tttcgcaggc accgcatttg ccaataccgc actcaaggga
    45181 tcggtttcga tcctgaagac tgtgactgtg taccggaaag cctgcattat gcaaagctgc
    45241 agcaatggtg aaacccttat cgccttctac tttaacgcca ttatacaaaa aaatgtgttt
    45301 ttcacattcc ggaacaggca ggatggggtg tttatcaatt tttgacatag tgctgaataa
    45361 taatttacga ctgcaaaatt agagcttaat tcaaaacaag catgctcaga actttgattt
    45421 ttgatagacg gttttatgag ataatgcaat gactcccatt catgagtttg aaattcatct
    45481 gaaaaaatgt aactttgcgc aaaagattga ccatatgggc agaattacca cctttctgag
    45541 catctgcatt gctttcacca ttatggcctg caatggcgga aacaaagatg aaacaaaaaa
    45601 caacccgcaa aaaagcacgg taaacaacaa ccttgaagcc gggaaaataa caattggcgc
    45661 taccgaagaa agttatgacc tggctcagcg gatagcttcg aattatgttg cacaaaacac
    45721 caacctgatt gttgaggtaa taccttgcaa aatcaatgaa gcaaaacaaa tgattgcaga
    45781 aaacaaagtg cagctgattg taaccggcgg caactccaac cctttcccag aatacgaatc
    45841 cacaattatt gccacagacc ttctgctact gacagttaat ttcaataacc cggcactgca
    45901 atatcttgtt atgcgcggac ttgatctgaa agatctttca ggaatttttt gtacaggaac
    45961 gattaacaac tggaaccagg tggataaaaa atcggaatca acgccattga aaccactggc
    46021 ggggccagac agctcaagtt caaataatat ggtccgttcc tttctaaaat cgaatctggc
    46081 tccgacagta acatcaagca tgagtgaaaa tgaactgaca agttctgttt cggccagtcc
    46141 cggatccata gtctttatga gtcaccggat gacgtataat cagaattctg gagtcagggc
    46201 aaacggcatt tacatcattc cggttgattt cgacggcaac aatgttgcca acgactcaga
    46261 actgatttat gacaatctga acatgctgaa aaaagcatac aacaaaggtg gattcccaaa
    46321 atctcttgtc cgcaatcatt atttcattgc aaacggcaaa gcagagcgtt cagatattgt
    46381 aaaacatttc acccattacg cacaaaaaga agcttcgaat gttatttccg gagccgggta
    46441 ttttcaacca cttaaataaa aaacaatatg aaaaaaattc tcgtcattct cattattgca
    46501 gctggactgg tgtcatgtca ttctacaaaa aaagcaaaca ctgattcttc cgccaaaatg
    46561 ccacaggcaa caataatgac caatttcaag gatacagtca gctacattat cggaactgac
    46621 attgccaaga gtttctctca aaacagcatt gacattaatc ttgatttgct tttcgaagga
    46681 attaaagacg gatacaatgg aacagactca ttattcacca aacagcaggt gcaggaaatc
    46741 atgggaaaat tccagaaaga aatgcagcaa aaacaacagt tggaatctgc aaaacaagca
    46801 acactcaaca aagaaaaagg gaaactatac atggatgcca atgcgaaaaa tgaaggcgta
    46861 atgcaaacag catctggact tcaatataaa attctgcgcg atgcgacagg tccaaaacca
    46921 acagccacca ataccgtaaa agtaaactat gaaggacgtc tgattgacgg tactattttc
    46981 gacagcagct atgaacgcgg agaacctatt gaatttggac tgggtcaggt tattaaagga
    47041 tggaccgaag gtctgcaact gatgagtgtc ggctcgatgt atgaattggt tattcccagc
    47101 gacctgggat atggcgatcg tccgatgcaa tcaattcctg ccggttcaac actgattttc
    47161 aaagttgaat tacttgattt caaataaacg caaaaccaat cgaatgaaaa aactaataat
    47221 gcttctgata gccgcagctg ttgttactgc ctgcagcacc agcaaaaaag acaaagaaac
    47281 cactgcttca actgaaaacc aaacgcagga atacattaca acagccagtg gtctgcaata
    47341 caaaattctg cgcgatgctg ctggcccgaa acctggccct acaagccgtg ttacagtaca
    47401 ctatgagggg aaactactcg acggaactat tttcgatagc agctacgacc gaaaaaaacc
    47461 aaatacattc ggtctcgacg aagtgatccc gggatggact gaaggtgtgc aactgatgag
    47521 tgccggctca atgtacgaat tcattattcc gccagctctt gcctatggga gccgtgaatt
    47581 gccgggcatt cctgccaact caaccctgat tttcaaagtt gaattacttg aaatcaaata
    47641 aaatttttca ctttctgaag cccgctctca gcgggctttt ttaatgatta atgttcagct
    47701 ttagtttgta aagttgaaag ttcataaagt ttataaagat actttaaaaa atctatccgc
    47761 gctctttgcg cgatactttt gtggaatctc tctgcgtccc tgcgccctct gcgcgatgct
    47821 tttgtggaat ctctctgcgt ctccgcgcga cccaactgaa tcagtcttca tcgcagatga
    47881 agggtgattc aattagtgtt aatcgaaagg gcttattaag taatgaaaca gaatttcgcg
    47941 tagattcgcg tcattcgcgg aagacttgca tgaggttcct ctgcgtctcc gcgttctctg
    48001 cgcgatactt ttgtggaatc tctctgcgtg ctatttgaag actctgcgta tccagacagg
    48061 cataaccaga cttcccacca acagataata atagaaagtc agcaagcgcc atataagtgc
    48121 caaaaccggc gccagaccta caatcacaaa cattccgaga taatcagaaa aaatccattc
    48181 ggcaataccg ctggctccgg gcgtggggct agccagcata ataacccaca tatagagctg
    48241 acgataatac agttcaaaat gatcaactcc cggcataaat gccataatga ggcaattaat
    48301 aataaaaaag cgagagaccc atgcaatcag agtaacaaaa aacaattgaa tccagaagcc
    48361 gaatgattta ccacgcattt ccctgctact tgccttcaaa tcacttccca tccggttggc
    48421 acggcctttc cagcgacgca aaaatttaag actaaaaata cgacgtagga tttttgcaaa
    48481 aaacgttgga agaaagaata tcgatactga cagaaaaaag gccaaagatg caagataaat
    48541 atatgcgccc cagacaatcc aatccggctt caaatcaaca gcaaataagg aatttccata
    48601 tatgcctgcg atgtaaccaa ccaataacag aagcggaatc aaaataataa aatacaactc
    48661 atcaagcagc gacgaaatca tcacaacagc agtactgcgt cccaatggga ctccggtttt
    48721 atgaatagta atcaatgcta cgccagctcc gccaataaat gcaggcataa cggtgtttcc
    48781 gaattcccag agcataatgt tttgaaaaga ttgattccaa ctgagtttct tttgtgtgag
    48841 aatccggagc cggagcatat tcataaagtc gcgcagcaac aggcacagca cagcaataaa
    48901 aatccagaaa atggtcttgc cgccccaatc gagctgcgac aacatattcg catcaaagct
    48961 gcggccgatc atataagccg aaatacttag tcccagcgca atcggaataa ggatacgcca
    49021 ggtggagaaa tatttcagga aggaaatttt gttttgagtc accatggaaa tacgaaggta
    49081 gtaaaatacg aaatacgaaa tcagaaattt gaaatacgaa ataagatgtg gaaatttgtt
    49141 gctcacgtaa aaccacgtaa aacagcttgc cacagcttgc cgggttttac gcggtttcac
    49201 gtggtggcaa gctgtgacag attcgcgaat gtgagacttc gcttgatatg aaaatgcgtg
    49261 aagagggata gtgatgaaat ccgtaacgat tcccgctttc atcgggataa attccacttt
    49321 gtaacgtgta acgaaagaat gtttaaatat ggaaatgaaa tcaaaacaaa aattcgaatg
    49381 tggcaatcta tctttcatca acaattcccc gttgatataa tctgctgcac atacgaatta
    49441 caaagccgtt ttcgttgtat ttttgaccta tgcgtaatat tatgcccatc cggaaagtcc
    49501 ttctaatcct gctcgtgcca ctgatgtttt catcgtgcac gggactgcgt gagcatatac
    49561 ggcagcgaca actggagaca catggtccac cgaaaagcat tagagacaat cgcaacgacc
    49621 gcacagagcg gaaagacaaa acccggcgcg acaccctttc caacatcaca gagagaaatg
    49681 ctccgacagg catttatgcc aaatacggag agaaatggaa agtagaatta agcggaacag
    49741 aagatgtgaa gttccttgaa gaaattgatt catggctggg cacgccctat gtgtacggcg
    49801 gcgaagacaa aaaaggcacc gactgcagcg gcatgatcat gaccatgtat aaaacgttgt
    49861 ataatctatc gctcaaccgc agcgccaacg acatgcagaa aaatgtgcgc ttcatcgata
    49921 ttgacaaagc caaactgggc gatgtgttgt ttttcaaaat caacggtgat cgcgtaagcc
    49981 atgtagcttt gtatctgggc gaccgccgat tcattcacgc caccaccagc aaaggcgtca
    50041 ttatcagcag tcttgacgaa gattactatt cgaagcgata tttcaaatgc ggccggataa
    50101 tagcgatgga aagacaatag cgcggaatac gcgcctattg taagtttgta aagttataaa
    50161 gttgaaagtt tataaagacg acgttaagca tgagaacgag accatggtta agatcaagat
    50221 tgagatcaag atgtttcatc atagctgaca gcttcagctg tcagaattca gaatgattag
    50281 agccgaaggc ataaaagaag ctgcgacttg tcacgtttta cgtgaagcac attaataatt
    50341 cgccgggagg cgataaatat taatagcccg catttatcac gtttcacgtg atgcccttta
    50401 gggctacggg gacaagcaag caatgattca gtggccagta ttcagtagtc agtaaaattt
    50461 tcgctcacaa ctgaccacta tataaaactc gctactgact ccgccgcggc ggatcacaac
    50521 tgacaactgg ctactcacaa ctggctacta agtctagttg tccctcagaa caaattcatc
    50581 atattccagt tcaaagcgca gtttgtgttt cgattcttcc tgagcaagat actgaaaaat
    50641 attgcggatg gcgctgtttg atgcttctga agccagtttc gtgtaaagct taaaagctgc
    50701 tttctcgcgc gccatggcca ctttcagcgc ttcggcataa gtgatgtttt catccacatc
    50761 gggataggca agataatcgg caatgttcaa tgccatcact ttttccttcg gcaggtcgaa
    50821 gttttgctct tctttaatct gagcgatttt tgccttgtga ccgatttctt cacgcgcaaa
    50881 ctcttcgaaa atctgcttca tctcatcgtt ccggcttttg gcggtcagtt tggtgtagaa
    50941 gtcaactgcg ctttgctcct gattcattgc aaagtcgaga atgtgatcca tcgattcgaa
    51001 tttttccatg gcctgtcgtt ttaagtgatt acacaattat tttctcatca agtctgcaac
    51061 ctgcttctga tgccgtaaaa caagattgtc ggcaccaaat acaaacatgt tgcgcggatt
    51121 atcgttgtca gtattattga aagaaaacag ctcttccttc tgagcaggaa gcaatgaggc
    51181 gaattcaaga aatatttcat ctgttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51301 nnnnnnaaca gcatcagatt attgatttcc aaagcacaat tcgacgatgt acctgcttcg
    51361 gtataaatca atacagtttc tgcaatatga ttgaactcat cagctaaaga ctcgagtgca
    51421 tcctgagcca gtcctgatgc ggcaatcagc gatgcatagc tttcctttaa taaagtttgt
    51481 ttgtactcgt caaatccttc cgtgcggcca ttaatgaata acgcattctg ttttcctgaa
    51541 gtcagcagat aatgattcat cgctttgatg aatgcatatc gcgatttcac ggctatgatg
    51601 cggtcagcct tatgatccat caggtggtta tcaccttcaa taattaaagt cacaggaatt
    51661 tcagatttat ttcttgccac ctgtaccgca aaacccgcga caccattcga tttgatcatc
    51721 tccggattca gcacccagat ggctcttgca aatttcagtt cattgaacgg aacgcttgcc
    51781 atactgtttt cggtataccc ttttccacgt tccatataat ggaaggaaga aatattattt
    51841 gtttttacac cggctctggc aatcttctga atataatatt gctcctcgtt cgaaagtcgc
    51901 gctccgacag tgaatgcaga ttccgatggt ttcgaagatt taagttgttt cgcaatgatt
    51961 tcaaacgctt cgctccactc aatgtctttc caggagccgt tttctttcac gcgtggttta
    52021 agaagacgat tttcatcatt catgtactga tatccgaaac gaccgctgtt acacagattt
    52081 ccgccctgat tcacctgacc tttcgaacct ttcacgccat atacaaatcc tccgcgatgc
    52141 tgtacttcaa gctcgcagcc aaccgagcaa taattgcaaa tggttttaaa cgattctgtt
    52201 ttcaccggac ccggcttaaa cagcttgttt tccgacatgg ctccagtcgg gcatgttgaa
    52261 acacacatac cgcagctttc acatttagtt tctttaaggc tcatgcccat tgcaggagca
    52321 acatatgttt caaacccgcg attcacaaaa cccaatgctt cagcgccgac cacttccttg
    52381 cagatgcgga tgcaacgtcc gcacaaaatg catttattat tgtcgatttc aatatatgga
    52441 tgcgaaaaat caattttata ttcattaaat gatcctgaat aatgcacctg attggcgttg
    52501 tattccgatg catacttttt caggtcgcag gtgtagagcg cgccacatcc acattcaaga
    52561 caacgggctg tttcattttt tgcaatctct tccgaagcat atccaagttc gacttctgaa
    52621 aaattcatgc ggtcttttgg atcaagcacc ggcatttctt cacgcagctg atgtttgaat
    52681 ttaccaacat attcgggctt ttcctgtttg cgaaaatttt ccttacgact gaaaaattct
    52741 ttggccggcg gcacaatggc atctccattc agatattgct cacaactatg aaccgcaagt
    52801 ttcgcctgtg caatggcagc aatggcagtg gcagggccgg ttacaccatc acctgctgca
    52861 aacacgcccg gaataccggt ttccaacgtt tctttattgg cttcaatgtc gccccatttg
    52921 gtaattttca attgaccccc tgtagcaaat tcattgatat cattaatgaa attgacatcg
    52981 gttttttgcc cgatggcagc cagaatataa tcgcattcaa tttcaaaatc cgacccttcc
    53041 accggaacag ggcgtctgcg gcccgatgca tcgggttcgc ccaattccat tttctgacaa
    53101 accatggatt tcaatttccc gttttcgtct ttcagaattt ttacaggcgc agtgagaaac
    53161 atgtagttca ctccttcgag tttcgactca tgaatttcaa tcggattggc aggcatttca
    53221 gcttcggtac gacgatagac aacatatact tcttccgatc cacagcggcg cgatgtgcgg
    53281 caacaatcca tggcagtgtt tccgccgcca acgacaacaa ttttctttcc tttaaaatcg
    53341 tagcgctgac cagtagcttc catgtttttc agaaaatcga ttccgctgaa aacgccgtcg
    53401 gcatcttcgc cttcaacgcc taccaatgta cctttctgcg atcctatagt aagaattgtt
    53461 gcattgtatt tttctttaat ttccttgtac gaaatatttt ctcccagttt tttttcggta
    53521 taaatattaa cgccaagttc cgttattgtc gaaatttcct gatcaatcac cgcattcggt
    53581 aaacggtatt cgggaattcc ataacgcaac caaccacctg catgtggcat tgcttcaaag
    53641 atatcgacct gatgtccttt ctgagcaagc caatatgccg ccgataatcc accgggacct
    53701 ccaccaatca cggcaacact cttccccgtt gcaggcgcta cttccggacg gtaatgatta
    53761 tctgatttca gatcctgatc ggctgcaaac cgtttaagat aatcgattcc gacaccattg
    53821 ttttcatcga gcaaattgcg tcggcaagcc agttcgcagg ggcgcacgca cacgcggcca
    53881 caaatagcgg gcagtggatt acgttccttg ataagtccaa tagcttcgct gtaaagccct
    53941 ttttcaatca atgaaatata accctgcaca tcaacattgg caggacaagt ttccttacaa
    54001 ggtccgatac aatccgcata gtggttactc aaaagtaatt ccagcgccat tttgcgactc
    54061 ttcttcacat cttcgcgttc tgtatcaatc accattcctt cggtaacttt tgttgaacat
    54121 gaaggttgca aacccttcat tttttcgaca tgaaccacac acacaaagca cgatgaaaac
    54181 ggctccagcc ggggatcatg acaaagtgtt ggtatgtgat atccataacg cgttgccaat
    54241 tccagaatgg tttcgccttg ttttgcctga acgttttttc cgtttaaaac gacattgatc
    54301 gtatccatga ttttcttttt accagattaa gacaaaataa ttgcatcaaa cttacatttg
    54361 ctgaagcaca caccgcactt gatacagagc gactgatcga tcaggtgtgg caacttgcgt
    54421 tcacccgaaa tggcattaac cggacagttt ttagcacaca cggtacaacc agtacattta
    54481 tcagaatcga ttgtataggt aagcaatttt ttacaactat gcgccggaca ttttttatca
    54541 taaatatgcg cttcgtactc gtgtctgaaa tacttgagtg ttgtaagcac gggattggga
    54601 gccgtttgcc ccaatccaca caaactgctg gtttttatct gttcgcacaa ttcttcgagc
    54661 aattcaatgt ccccatcgcg accttcaccc tcggtaatgc gggtaaggat ttcaagcata
    54721 cgttttgtcc ccacgcgaca gaaagtacat tttccgcaac tttctttttg tgtaaaatcg
    54781 agaaaaaagc gcgccatgtc aaccatacag gtgttttcat ccataaccac caacccacct
    54841 gagcccatga tggctcctgt agctgttacg gaatcataat ccacaatggt atcaagtaaa
    54901 tgttccggaa tacagccacc cgaagggcca cccatctgca cggccttgaa ctttttgttg
    54961 ttctgaatac cgccacccag tttaaaaatt acatcacgca acggaatacc catcggcact
    55021 tcgacaaggc caccgtggtt gatttttccg gctaatgcaa acactttggt acccttgctt
    55081 ttttcggtgc catatgccgc atatttttca ggaccgttta aaataatcca ggaaacatta
    55141 gcccatgttt caacgttgtt gatgttggtt ggttttttaa ataaacccga cacagccgga
    55201 aacggtggtc tgcggcgcgg cattccgcgt tcaccttcaa tcgaagccat gagtgcggtt
    55261 tcttcaccac aaacaaaagc accagcacct tcttttatat acaaatcaaa attaaatcct
    55321 tcgataccaa aaatattcct tccgagatat cctctttcgt gcgcctgacc aatagcgatg
    55381 ttcagacgtt tgatggcaag aggatattca gcgcggcaat aaatcacacc atcgctggcg
    55441 ccaatagcat aggcgccgat aatcattcct tcgataatgg catgtggatc gccctcaatg
    55501 agactccgat ccataaaagc accagggtca ccttcgtcgg cattgcagat aatatatttg
    55561 gtttcgtttt tactgttgta ggcaaacttc catttcatac cagtgctgaa accaccgcca
    55621 ccgcggcccc ggaggccaga gtcgatgatc atctgaataa cgtcttcgcg cgaaatactg
    55681 ctgctgaaaa ttttctttac cgcttcgtat ccaccctggc gaatataatc gtccatgttt
    55741 tcaggatcaa tcacgccgca gtttcgcagg gcaattttca cctgtccttt ccaaaaagaa
    55801 tcatcctttg aaacaaacaa atcggtacgt acaatgtatt cgcgcactgg ttcgccattg
    55861 cgaacatgtt tattcaaaat ttcgagcgct ttttccgcat ctacttcacc ataaagataa
    55921 cttccgtttt catcaattac ctctacaagc ggctcgcgga aacacattcc gatgcaaccg
    55981 gtttgttgca gatcaaattt caggtgatcg gtgttttgta atcctttgat tgtatcaaaa
    56041 actttgcgtg ccccagcggc aataccacaa ctggccattc ccacgcgaac taatgtctga
    56101 gccataacaa tgtctatttt tgtttttcct gcattctgat atcattcaca attttaacag
    56161 cagacttacc cgtaagtttt ccataggttt ctccactaat catcattacg ggggccagcg
    56221 aacaacaacc cagacaagcc acactctcaa gtgtaaaaag tccatcttcc gttgtttgac
    56281 catctttgat ttcaagtgcc tcttcaatag catcgctgat ggcagtagca ttctgcacat
    56341 ggcaggcagt accatgacag actttgataa tatgtttgcc gacaggatgc aaccggaatt
    56401 gagaataaaa agttgctacg ccaaagatat cactaacagg gatatgtaat gtttcagcag
    56461 ctttaacaat agcaacagat ggcagatagc cgtaaacatc ctgaattttc tgcaagacag
    56521 gaataacagc gcctttatga ttctgataat ggtgaaggat ggagtcaata agcgaaagat
    56581 caacttcagg ggctttggtt tcaacatgtg caaccggcac gcgttcaatt ctcataaaac
    56641 aaagatttat gcacaaaatt agttattata tacaattgta aaaatgattt tttgatcatc
    56701 cgcaataata ccagaaaaag cttcagagac tgtttgaaat ttatttcttt gacaaaagta
    56761 acacgacctt caatagtccg aaatgcaatg gaggacatgg aagagtcggt tgcagagatc
    56821 tctgcccaac tattccatgt tcttcgtgga ttttatactg cagggcagta ctgcgaacta
    56881 tggaatgttg gactacctga gtcgatcact ggcattatta aggatgatca ataatgattt
    56941 taaaaaaaag ccccggaaaa accagggctc tttataataa ggcaggacag gatactaaac
    57001 aacaccctga tcgagcatag cattggcaac tttgaggaag ccggctacgt ttgcaccctt
    57061 agcataattg atatatccat cagcttcttt accgttttta acgcaagcag catggatatt
    57121 attcataatt gaatgcagac gttcatcaac ttctttggca ttccaggaaa gtttcattga
    57181 gttttgggtc atttcgagac ctgaggtagc aacaccaccg gcgttaacgg ctttaccagg
    57241 gccaaaagga atcttggcag cgtggagagc ttcaacagct tcgggagtac aacccatgtt
    57301 ggaaatttca gcaacaatct taacaccatt ggcaataagc atttttgctt cttcaccatt
    57361 gagttcgttc tgggttgcac aaggcagagc gatgtcaact ttaacttccc acggacgttt
    57421 accggcgaag aattttgaac ccgggaattt atcagcataa ggtttaacga tatcctggtt
    57481 gctggcgcgg agatcgagca ggtaattgat tttttcacca ctgattccgg cttcgtcata
    57541 aatgtatcca tcaggaccac tgacagtaac aactttagcg ccgagttcgg tagctttcaa
    57601 agctgcgccc catgctacat taccaaaacc tgagagagaa acggttttac ctttgaaatc
    57661 ggtacccatg gttttcagca tctgctcaac gaaataaatt ccgccgaaac ctgttgcttc
    57721 aggacgaatg agtgaaccac cccagttgat gcctttacct gtgagaacgc cggtattttc
    57781 gcgggcgagt tttctgtaca taccgtacat aaaaccaatt tcgcggccac caacgccaat
    57841 atcaccagcg ggaacatctg tttccgggcc gataagtttc cataattcga gcataaagct
    57901 ctggcagaaa cgcataactt caccgtctga ttttcctttt ggattgaaat cagaaccacc
    57961 tttggcaccg cccataggca gggttgtcag actgtttttg aaaatctgct caaaacctaa
    58021 aaatttcagg attgaaaggt taacacttgg gtgaaaacgc aaaccacctt tgtaaggtcc
    58081 gatagcattg ttgaactgaa cgcggtatcc gcggttaaca ttcactttac catcatcacc
    58141 tacccatgga actttgaaca tgagagttct gtcgggttca acaaggcgtt caatgatgcc
    58201 tgctttgtca aattgcgggt tttcgtttac aacttcttcg attgtttcga gtacttcacg
    58261 tactgcctgc aggtattcaa cctggcctgg atttctctgt tccagatcct gcatgatttg
    58321 tttaacgttc atattgtaag aattaagttt ctgatgcaaa tgtaagtgct attcattcat
    58381 tgccatcaaa taccatcatt caaaatacac caacaaaaaa tgtaataaat caccccaggt
    58441 cttgacaaaa aacaaatttt gccatgatat attttatagt acctttgcaa actattatta
    58501 ggtaacaatc ctttatttcc acatagtcca ttgataatga acacacttgg attacacgac
    58561 cgcgcaaagg aactcaattg cctgtacaag gttgacgaag ttctgcaatt tatcgacaaa
    58621 gaagtaccac aggccattag tgaattattg gatgttatcc catccggatg gcgatattct
    58681 gatttatgcg aaggtgaaat tatccttggt gatcatattt tttcacgtcc cgggctacaa
    58741 cgaaccgaac ttaaacaaag cgcactcata aaagcttatg gcgaaatcat tggagaaatc
    58801 agagtgtatt ataccaaacc ggttaaatcg gaaaaaggaa tttttctggc agaagaaatg
    58861 caaatgctgc attccattgc tgaaaaaata gccatgtttg ttactttgcg caaactcaaa
    58921 cccagtcatc ttgaagaaga agccagtgaa gatgaagctg aaattctcaa aaaccttcaa
    58981 catgatttcc ctgaaatgat ggactggctc aaaaacatgg gactcaaaaa acgcgaagcc
    59041 gttcaaattt tacgaaatcc tctcaatttc cgtaaaggcg aaaccatcgg caaacagggt
    59101 gctttcactt cctattttat actactcgcc aaaggcgcaa caaaatcgta catcgaaggt
    59161 ccgggaagca aaagctattc atttatgctc acgctgccat ttgaattcat tggaatgtca
    59221 tcacttttcg gaaatcatta ctatttttca acggttgctc tcattccatc aacagttttt
    59281 ctgattgaaa aagaaaaggt gattaaactc atcaacgaaa atccgaaatt caataagagc
    59341 attgttgcgt ggctctgcga cagctataaa attcttttcc acaagatgag ctgcctcagt
    59401 ctcaaacaga cgatcggacg tatggccgaa gtgattctgt acctgtccaa cgatgttttc
    59461 aaaaacgagg taattcctca ctatatttca agaaaagacc tggcagaact cagcggcatg
    59521 agcaacgaaa atgctgtgcg tattctcagc gattttaaaa acgaaaatat tctgcgcgac
    59581 acgccagaag ggctgcagat tttgaataaa tcattgctgc aaacaatcag cattaccgga
    59641 taacgcatag ggcatcacgt aaaacgtgac aagtgcggca tgcggcatag agcatgcggc
    59701 gcttagcaag ttgaaactta tgatgttata aaagtgacag aaatgcatgc agcgttgagg
    59761 cataatgctt aatgcacaag gcacaatgca ggctttgcaa agtctttcac ttttatcttt
    59821 tgtcttttta cttttatctt taatcttcca ccgtaagaat tcctctcaag cggatatcgt
    59881 tggatgaagc accgatcatg atgcggaaac taccgggctc aaccacgcgc tgcatatgct
    59941 cattcagcat actgagcatt tcgggagata tttcaaatgc aatgtttttg ctttcaccgg
    60001 ctttcagatg cacgcgctta aatcctttca gctcaatcat agggcgagca acggaggaca
    60061 gttcgtctct gatgtaaagt tgcacaacct cgtctccatc tacctttccg gaatttttca
    60121 atttaaagga aaccattgtg ttttcattgg agctgaaact atctttactg aatttcaaat
    60181 ctgaatattc aaatgttgta taactcaacc caaacccaaa aggaaacatg ggctttccgg
    60241 tcatatcaag ataatcgtcg ccacggccag ttggtttgtg ataatacaaa tacggcaatt
    60301 gtgcttcatc gaccggaaag ctgattggga gccggccggc gggattgtaa gcgccgaata
    60361 aaacatcagc caccgcagta ccacccagat cgccgggata ccagacttcg accagtccat
    60421 caatgtcatc aataaaatca gtcactacaa caggcccacc acctacaaga acaacaatga
    60481 cctgtttccc ggtttcagca attttatgaa tcatttcttc ctgccggcca ggtaaacgca
    60541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gattcacggg cagcgttcag
    60661 aggaagatcg ggtaatggaa catcgcgcag aatcctttct tccacatacg gattttcaaa
    60721 caatcccaat tcaaacttca cgcgcaatac tcgcgcaacc gcgctatcaa tggctttcgg
    60781 gtcgatcaga tttttttgaa acgcttcata aaacaatgta taatgatcgt acgatgtctg
    60841 gaaaatgaca tccagtccgg cttctatcga ttgctttgtt gcgtcggcat aatcctgtgc
    60901 agtaaaatgc aacacattgg ctccgcctgt tgcgccagca tcgctgatga caaaaccgcg
    60961 gaatccccat tcatttttca gtttttctgt caacagaaaa tggctctgcg agcagggttt
    61021 accattaaat gaattgtaag cagtcatgat tgaagtcgca agacctttct caatgaccgc
    61081 tttaaatggc ggataaaaaa cttcttccat catccgctca ctgtattgca ccggatagct
    61141 gtcgcggccg ccatcaccag agttggcaat aaaatgtttg ggtgtagtaa tgattccctg
    61201 tttttcaaac tcgctgatat aagccagcgc catttgtgtg accagaaacg ggtcttcgcc
    61261 gtaagtttcc tcaaccctgc cccaacgcgc atcaacagct acattcagca ccggcgacaa
    61321 aatcatgcgg atgccacgtt cttttacttc aaatgcaatt tgctctgcga cgtcagccat
    61381 cagtgcggta tcgaaagtag cagccagcgc aatggattgc ggaaaagaag tagccccttt
    61441 tcgcaccagt ccgtgcaaag cctcatcaaa tggtattatc ggaataccca gtcgtgtttc
    61501 ttcaacaaaa tatttttgcg cgcggttgat ggcttttacg gtttcgaccg gaccaacacc
    61561 tgaataattc atcatttgct ccgctccatt gccggcttga ccaacagcag caaactgaaa
    61621 tccgaaaata ccatgcggat acttttccaa atctacatcc aatgcagcag gaatcatata
    61681 aagctgccag aatttttctt ccaaagtcat gcggttcaat aaatctgcca cacgagcttc
    61741 ggtgctcagc cgcgggtttt tgtaaggcaa tgcatcctgc gccctgagcg ggatttcaga
    61801 tacagcaaaa aacaaaataa ctatcagaaa aaaagaacgc atgaccattt atttaagcgt
    61861 gatgcggaat accgaaatat aagcagtcgg acgctttgct aaatcctgca tcgaatcagg
    61921 catgcgcaat gtatagccat cagcactgaa agatgttttg attttctcct ttgaaccaag
    61981 catctgcact ttgtagtttt tcttttctgt gatgcatgac aatgtcaacg atttcccggg
    62041 ccatgttaaa gcaaaaacat acagatattt tccatctttt gattgagtaa aacggattgc
    62101 atcaccatct ttaaaatgtc tccaggagcg gcttccgtaa attgcttcgc cattgaccga
    62161 catccatttt ccaatttcat ttaatgttga aatagctttt tcctcaaatg tgcccttcgg
    62221 tgtcgggccc acattgagta agaaatttcc acctttagaa acaacatcaa ccatagacct
    62281 gataatggtt tcagctgatt tgaatctggc atttggtacg aagccccagt tatcagtcat
    62341 ggtcatgcag gtctcgaacg gataaggtaa aatggaatca ggtatgcttt gctcaggggt
    62401 gcggtaatct tcgaaaggcc cgtgaacagt gcggtccacg ataagaatac ctggttgatt
    62461 ttcgcgagcc atggcagcaa tgcgcggcat atcaatgtcc tgttcgtact taggaatgtt
    62521 gtagccccat gagcgcacct cttcgttgat tgttgaatca ggacgcaccc agccaccgtc
    62581 gagccaaagg atatcaagct ttccgtattc cgtagtcagc tcttcaagct gattgtaggt
    62641 ataattctga aattctttcc agcggcccgg atattttcgt gtgtcgtaat tgcagtttct
    62701 gtcgggcgtg gccagcagcg gcgtccagta agccggacaa tgccagtcgg gtttcgaaaa
    62761 ataggcgccc accataaaac ccttgttgcg gaaagcttc
//
LOCUS       DAOPKE010000006        62643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_480_length_62643_cov_12.137055, whole genome shotgun sequence.
ACCESSION   DAOPKE010000006 DAOPKE010000000
VERSION     DAOPKE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_62643_cov_12.137055"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PKY63_01475"
     CDS             complement(<1..950)
                     /locus_tag="PKY63_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPE99310.1"
                     /translation="MKKSNQDIILWIFRILVSGLFLLSAYGKFFPLESALHVFAKQIV
                     GLGIADWCIAPLLARAIVGFEIFLGIAILQNNFLRNVIVPSTFLLLLVFCIHLSWVFY
                     TSGNVGSCGCFGELIPMPPLEAIVKNLLTMAMLVYIFLKVKKKKNDNFLVPAVIFLIV
                     YLVILILQPPKFNCNTVEASQEIVSDTIVAEVDSVLVADTVIDESGNEIITEMKKVVE
                     NQNVIEGRKKVNSIFTKYTKFNTGAVDLNEGKKIVCLYSFDCEHCQESSKLLLELKNN
                     HNNFPPVYMLAFGEESQSTDFFKGAGGHIPYVVVEPQEFF"
     gene            complement(990..1208)
                     /locus_tag="PKY63_01480"
     CDS             complement(990..1208)
                     /locus_tag="PKY63_01480"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HPE99311.1"
                     /translation="MNSKKPMLFFLSLMILIMTMVVSGMGTLMNFGFASGFFLHWMKA
                     WAVSYITALPVALIIAPVIRSFVLRKVQ"
     gene            complement(1216..1629)
                     /locus_tag="PKY63_01485"
     CDS             complement(1216..1629)
                     /locus_tag="PKY63_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPE99312.1"
                     /translation="MNIIKIALFCLLLFVCYNQAVSQALQQKTSYDVMQMLPELDSAS
                     SVIIDGRDSAKFCNGHLKNAVCLDAFSAEASELLSQYLNFGTIVVYCTTKNRTETIVE
                     MLQKLSYGGTIICMMDGLTGWIENGFPVEKEVKNQ"
     gene            complement(1632..2975)
                     /locus_tag="PKY63_01490"
     CDS             complement(1632..2975)
                     /locus_tag="PKY63_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPE99313.1"
                     /translation="MEKKKELKILFWIAVVFAAAFFLPIENTRFNTAIAATLDLVKWY
                     AREHVVLCLLPAFFIAGVIAVFVSQGSVLKYFGANAKKWLAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARTIGAVLFSVI
                     IGVSMSLIYRKEEKLKREEQMNIQLPPEKRPMWQTSFHFFVLVLILVFANWGKPSADD
                     TSSTWFYIWSYKWWITGGFSILLMYSLIRILKIHWMWVIAGGILTALSGFLAFEFIEN
                     AKLAPLVPMLIGIAALSIMTLFDKRDQDNKEWTYSTWGFAKQILPLLAVGVITAGFLL
                     GSTHDDVSIAGVIPNEWISSLVGGNSVFSNFFASIVGAFMYFSTLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVIGTQKTVVYVLLVIAMATLSGLIYGFFF"
     gene            complement(2986..3693)
                     /locus_tag="PKY63_01495"
     CDS             complement(2986..3693)
                     /locus_tag="PKY63_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPE99314.1"
                     /translation="MEFLQTWADNAQYPIFAAFALGLMTAISPCPLATNITAIGYISR
                     EISSGRKIFYNGLLYTFGRLVSYSGLGIIIYFGASTFKVAKLFSQYGEKFLGPLLIVI
                     GVLMLNVIKIRISKNGGSLSEKVEQKAAGGSSWWAFMLGVLFALAFCPSSGVFYFLML
                     IPMTVASPDGLLLPPVFGVATALPVILISWLLAFSMSGVGAFYNRIKLFEKWFRLVAA
                     VLFIAAGLYFVIVLWII"
     assembly_gap    3919..4018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4129..4665)
                     /locus_tag="PKY63_01500"
     CDS             complement(4129..4665)
                     /locus_tag="PKY63_01500"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPE99315.1"
                     /translation="MQRKIIFLFAVIALLMLASCGGNKQETAGNIDSVQTETIDSVVS
                     DSVISDSGKQVSGPENNGAFMPETPSVTVMLFHATRKCASCIAITDCVNAVLKESFSA
                     ETQIKKVRLNEINADDEKNEAICEKYEAFGTALFVTRTFKGKETITDLTAQAFKLARS
                     KPDDFKKLLREQIQKDLK"
     gene            complement(4650..4985)
                     /locus_tag="PKY63_01505"
     CDS             complement(4650..4985)
                     /locus_tag="PKY63_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPE99316.1"
                     /translation="MKKKVEFKPEHKELAMYAKALSHPARVYIMERLIQENRCDGVGC
                     CTSGEMITEVPIARSTLSQHLKELRNAGLIQGEIELPTIRYCVNKKNWEKAKKLFEHF
                     LNPTTCKGK"
     gene            complement(4991..5230)
                     /locus_tag="PKY63_01510"
     CDS             complement(4991..5230)
                     /locus_tag="PKY63_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPE99317.1"
                     /translation="MEVKVLGTGCANCKNLEAAARKAVEELGIEANVVKEEDIMKIMG
                     YGILRTPGLVIDEKVVLSGRVPSVSEIKEIIANAK"
     gene            complement(5424..5744)
                     /locus_tag="PKY63_01515"
     CDS             complement(5424..5744)
                     /locus_tag="PKY63_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPE99318.1"
                     /translation="MLDVRDPTLTAFKQFGVPEFISCHHIKLQEFASHLPDKRVLIVA
                     DSVGLFSHEAMIVLKDLGFENIINLAGGLVEWERDGMPVVVDTNERLSGSCVCQLRPR
                     ERKG"
     gene            complement(6011..6868)
                     /locus_tag="PKY63_01520"
     CDS             complement(6011..6868)
                     /locus_tag="PKY63_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HPE99319.1"
                     /translation="MLPVNRKYFRKVFAAVSLLVLVYSCGNQSSGQVNNSVINKEGLT
                     IQERFGAPDGFVRPDADSTTFGFYLRSLPLKPGGEKVKLFDGREKSPDNVYCAVVDLP
                     IGFKNLHQCADAVIRLRAEYLWQQKKFSDIHFNFTSGFRADYSKWMSGKRISVDGNNC
                     SWVARSAASNTHDDLWNYMETVFMYAGTLSLNKELNTVNWDDMWPGDVLIQGGSPGHA
                     VIVVDMIVNETTGEKRFLLAQSYMPAQEIQILINPSNSEISPWYSLDCSNDIHTPEWD
                     FKKADLKRF"
     gene            complement(6852..7952)
                     /locus_tag="PKY63_01525"
     CDS             complement(6852..7952)
                     /locus_tag="PKY63_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPE99320.1"
                     /translation="MGRLRRQFPLKKRMVFNIHARYVRVQAKLHDLSYFFWECTSRCN
                     LACLHCGSDCKIIENAPDMPAEDFLNVCRQVAERYNPNKVMIVVSGGEPLVRKDLEMV
                     GTELKKMGFPWGMVSNGYAMTPERFRSLREAGLRSATISLDGLKASHDWLRGREGSFE
                     KAVSAVKLLVAEPGMVYDVVTCVNQRNLSELDEIRDFLIEMGVKRWRIFMIDPIGRAA
                     VNPELMLKNDQYRYVMDFILRTRAEGRIKAATGCDGFLGDYEGLVRDGVFFCRAGIHV
                     GSVLANGDIGACPNIDRGFVQGNIYKDDFLDVWDNRFKEYRDRTPFKTGMCKDCDLWK
                     WCRGDGMHLHEPGNPNPLECNFKKLYPDVASK"
     gene            complement(7961..8278)
                     /locus_tag="PKY63_01530"
     CDS             complement(7961..8278)
                     /locus_tag="PKY63_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99321.1"
                     /translation="MKILLHNIRKVWAWILCRSLILSGLGMVVLVSCDSKSDQSQAAL
                     KKEKNIDVLLSRQDSAMITEVEKIAVQKVEKPLVPDYNPAPIQPTGYGAMPAYELELP
                     EKN"
     gene            complement(8311..8721)
                     /locus_tag="PKY63_01535"
     CDS             complement(8311..8721)
                     /locus_tag="PKY63_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99322.1"
                     /translation="MSHKIRYRLLLLNARLLRNMLVIIGLAGFLSACSGSDKKDSQQN
                     PDSLNKVKEQQRLDSIAKVREDSLSQVHADSLAKAREDSIKKAKEQQGQYSSSTVTGV
                     PNLNGNKPITKYGVPVDDDKPFQTKYGPPTDFDM"
     gene            complement(8793..9095)
                     /locus_tag="PKY63_01540"
     CDS             complement(8793..9095)
                     /locus_tag="PKY63_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99323.1"
                     /translation="MNINSKFFLYQLNASLLKRLLILLSLPVVAVSCKSAEKQLSEPD
                     PQMPDSAIVAPADTTKPNYYNPEIQTDYGVIQITPKYDTARPVPLYGVKVPEVPKQ"
     gene            complement(9099..9497)
                     /locus_tag="PKY63_01545"
     CDS             complement(9099..9497)
                     /locus_tag="PKY63_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99324.1"
                     /translation="MNLRIAYRIYRLRARILRNMLAMLSFSALISSCNGVSEKQTAIN
                     KDSLKLVELNQQLDSVLNENETLKLRLDSIIEKEKQDSIADAKKQVSVYVAPVIPDDP
                     GPICEYGVPPVYEFEISPEQIQTKYGVPRN"
     assembly_gap    9652..9751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9815..10312)
                     /locus_tag="PKY63_01550"
     CDS             complement(9815..10312)
                     /locus_tag="PKY63_01550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPE99325.1"
                     /translation="MDFTLRPWRKSDLKYLLKYADNPRIAMNLTDMFPHPYTKQSGKR
                     FLKMARSADPVRIFAIEINDEAVGSIGLFPLSDIRRNNAEIGYWLAEPFWGKGIMTEA
                     VKQVVEYGFKTFDINRIYAGTFGRNTGSQRVLEKAGFTLEARFEKTYVKNGELEDEVV
                     YAIRR"
     gene            complement(10742..11831)
                     /gene="prfB"
                     /locus_tag="PKY63_01555"
     CDS             complement(join(10742..11767,11769..11831))
                     /gene="prfB"
                     /locus_tag="PKY63_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137305346.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPE99326.1"
                     /translation="MAGKDDVKTLKERLDALRRFLDIDRRRSDFREEEKKSLAPDFWD
                     NPKEAEKFLKSLSEKKSWITRYDKVSLAVDELDVALEFFAGGDMTQEEFDQTYSVAET
                     AIEDLEFFNMLSGEEDKLSAIITINSGAGGTESQDWAEMLMRMYVRWAERNNYKVTEL
                     DLQEGDVAGIKSCSLEIEGDYAFGYLKSESGVHRLVRLSPYDANERRHTSFASVYAYP
                     VVDENIDIVLNPADLDWDTFRSSGPGGQNVNKVETAVRVRHAPSGIVVECQVTRSQLQ
                     NREKAIQMLKSQLYELELRKKREAIDAIESSKKKIEWGSQIRSYVLHPYKMVKDNRTN
                     HETSNTQAVLDGDINDFIKAYLMQFGRA"
     gene            12045..13085
                     /locus_tag="PKY63_01560"
     CDS             12045..13085
                     /locus_tag="PKY63_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPE99327.1"
                     /translation="MDNFEEYLRQGEPDKAEKAKVWKTAVGLQDVDGLKPSDYLIETA
                     RQNIEGDITIDEVKQRIDTYYKQHPQKTDESRTEEADKVSARIAEILSEKTFTFSPAE
                     YLSIHRRLFTDVYKFAGKIRDYNITKQEWVLDGETVLYGSAESLKATLEYDFEQEKKF
                     SYKGLSQHEVIEHIARFISYLWQIHVFGEGNTRATAVFLIKYLRTFGFVVNNDLFAEH
                     SWYFRNALVRSIYKNHSRKVYATNEFLLRFLENLLLGENHVLRNREMHIQLADPANDP
                     VKSKNDLVNDPVKSSILNHLKQNPKANYIELAENIGCSTATIKRHIQELKKKGLIERI
                     GSDKTGYWRLVE"
     gene            complement(13163..13726)
                     /locus_tag="PKY63_01565"
     CDS             complement(13163..13726)
                     /locus_tag="PKY63_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPE99328.1"
                     /translation="MIIRAIAIGYAPRGTSGRNTGKQIADTINTTGLYSVVRHPLYLG
                     NFLMWLGLIIYVGSWEFLIFAVFFFWIYYERIMFAEERFIGGKFGQAFEDWAAKTPAF
                     FPKCSGYVKTGRRFNWRSVMRREYHGFFATILSFAIINFLKHLFYTKESRIDIEWMIG
                     LGAGLLIYLFVRFVVKATRWLEVKPKS"
     gene            complement(13727..13903)
                     /locus_tag="PKY63_01570"
     CDS             complement(13727..13903)
                     /locus_tag="PKY63_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99329.1"
                     /translation="MSMIREFEKSGNRLFKYRSFIPLVLYVFAALAIWLDKDEFIPYQ
                     EYWWSLICLGVSAI"
     gene            complement(13918..15210)
                     /gene="eno"
                     /locus_tag="PKY63_01575"
     CDS             complement(13918..15210)
                     /gene="eno"
                     /locus_tag="PKY63_01575"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPE99330.1"
                     /translation="MATIANVYARQILDSRGNPTVEVELVTSNGNFARASVPSGASTG
                     IHEAHELRDGDKKMYNGKSVLKAVNNVNKVIAPEIQGLDVTDQNQIDRLMIQLDGTEN
                     KSSLGANAILGVSLAVAHAAAAETNQLLYRYLGGVNANTLPIPMMNIINGGSHADNSI
                     DFQEFMIMPLNAETFNQAIRMGTEVFHSLKDVLKKRGLSTNVGDEGGFAPNLKSNEEA
                     LQVIIEAIEKAKYKPGVDVAIALDVAASEFYNTTDKKYHFKKSTGDKLSSKEMASFLA
                     DWTKKYPIYSIEDGMAEDDWDGWKIITDKIGANVQLVGDDLFVTNVNRLADGIEKGVA
                     NSILIKLNQIGTVSETIQAINMAHRYGYTSIISHRSGETEDTTIADLAVAMNTGLIKT
                     GSACRSERIAKYNQLMRIEDMLGGQSYYLGTDFAFYPY"
     gene            complement(15290..15841)
                     /gene="rplQ"
                     /locus_tag="PKY63_01580"
     CDS             complement(15290..15841)
                     /gene="rplQ"
                     /locus_tag="PKY63_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPE99331.1"
                     /translation="MRHKNGFNKLGRKTAHREAMLSNMASSLILHKRITTTLQKAKAL
                     RSFVEPVITLSKEDSMHHRRMAFKYLQDKEAVKELFREVSNKIADRPGGYTRILKTDM
                     RAGDNAEMCMMELVDYNEVMLGTKEAKKTTTGRTRRSKAKKSDETPAAETKLPKAEDV
                     KAVDVPEEKPADEQAPETDGAEK"
     gene            complement(15847..16839)
                     /locus_tag="PKY63_01585"
     CDS             complement(15847..16839)
                     /locus_tag="PKY63_01585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPE99332.1"
                     /translation="MAILNFQKPDKVIQLESDDRKGVFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFSITGVKFDNVLHEFSTIQGVVEDVVDIVLNLKQVRLKRQIEELNSEHVNA
                     VFQGQEVVTAGDLNKFLSGFQVLNPDLVICHLDPTVKLSFELNIEKGRGYVAAEDLKP
                     ATENIGYIAMDATFTPITNVNYNIENYRVEQKTDYEKLVLTIQTDGSILPKDALKDAS
                     AILIQHFALFSDDKIDIEPTEKAVVDDFDEDYLHMRHLLKTKLSDMDLSVRALNCLKA
                     ADVETLGELVSYNKNDLLKFRNFGKKSLAELEELVKSKNLHFGMNVSKYKLDKD"
     gene            complement(16858..17463)
                     /gene="rpsD"
                     /locus_tag="PKY63_01590"
     CDS             complement(16858..17463)
                     /gene="rpsD"
                     /locus_tag="PKY63_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPE99333.1"
                     /translation="MARYIGPKSKIARKFQEPIYGHDKFLEKKKYPPGMHGQSKRRRK
                     VSEYGVQLAEKQKAKYTYGILERQFKKLFLLASTKKGVTGEKLLQFIESRLDNTVYRL
                     GIAKTRDGARQLVSHKHIEVNGKVVNVPSFLLRVGDVVSVREKSKNLEIITESLSGHN
                     NNFSWLEWDGEKMAGKFLNYPEREAIPEAINEQLIVELYNK"
     gene            complement(17487..17876)
                     /gene="rpsK"
                     /locus_tag="PKY63_01595"
     CDS             complement(17487..17876)
                     /gene="rpsK"
                     /locus_tag="PKY63_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPE99334.1"
                     /translation="MAKQTKTVRKRKVKVEPFGNAYVKASFNNIIISLTNEDGQVISW
                     SSAGKMGFRGSKKNTPYAAQVSAEDCAKVAFDAGLRKVKVYVCGPGSGRESAIRSIHG
                     AGIEVTEIVDVTPIPHNGCRPPKRRRV"
     gene            complement(17899..18279)
                     /gene="rpsM"
                     /locus_tag="PKY63_01600"
     CDS             complement(17899..18279)
                     /gene="rpsM"
                     /locus_tag="PKY63_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPE99335.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYIYGIGRSTSRMILTKAKVDFDKK
                     VQDWDDNDLTAIRTVISDEIKTEGALRSEVQMNIKRLMDIGCYRGIRHRLGLPLRGQS
                     TKNNARTRKGKKKTVANKKKAPKG"
     gene            complement(18304..18420)
                     /gene="ykgO"
                     /locus_tag="PKY63_01605"
     CDS             complement(18304..18420)
                     /gene="ykgO"
                     /locus_tag="PKY63_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HPE99336.1"
                     /translation="MKVRASVKKRTPDCKIVRRKGRVYIINKKNPKFKQRQG"
     gene            complement(18437..18655)
                     /gene="infA"
                     /locus_tag="PKY63_01610"
     CDS             complement(18437..18655)
                     /gene="infA"
                     /locus_tag="PKY63_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPE99337.1"
                     /translation="MAKQGAIEQDGTVVESLGNAMFRVELENGHVILAHISGKMRMHY
                     IKILPGDRVRLEISPYDLTKGRITFRYK"
     gene            complement(18664..19425)
                     /gene="map"
                     /locus_tag="PKY63_01615"
     CDS             complement(18664..19425)
                     /gene="map"
                     /locus_tag="PKY63_01615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPE99338.1"
                     /translation="MLQKSDEEIELLRKSSLLVGKTLGVVSRLLKPGVETRTLDRAAE
                     EFIRDNGGVPAFKGYNGFPATLCISINDQVVHGIPGSRVIKDGDVVSVDCGAVLNGYV
                     GDYAYTFIVGETDRKTMDLVRVTHESLLKGIAEAVPGKCVGDIGHAVQLHVERNGFHV
                     VRDLVGHGIGQHLHEKPEVPNYGRRGHGMPLVENMVICIEPMINLGTWRVYQEADGWT
                     IRTRDGSNSAHFEHMVVVRDTPEVISTYEYIENRV"
     gene            complement(19431..20795)
                     /gene="secY"
                     /locus_tag="PKY63_01620"
     CDS             complement(19431..20795)
                     /gene="secY"
                     /locus_tag="PKY63_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPE99339.1"
                     /translation="MSRLVNTFKNINRIEDLKKRILYTLLIILIYRFGSYVVLPGIDP
                     NSLDALQNQGQDGLLGLINMFSGGAFSNASVFALGIMPYISASIVVQLLGIAVPYFQR
                     LQREGESGRKKMNQITRYLTVIITAAQAQGYIANLASQIPAGGIYPFDAAISQVPFLF
                     RLSSTIILTAGTLFVMWLGERITDKGLGNGISLIIMIGIIARLPFALLAEFASRMEVA
                     GGGLVVFIVELVILVLVVVMTILLVQGTRKIPVQFAKRVVGNKQYGGVRQYVPLKVNA
                     AGVMPIIFAQAIMFIPITLAGFANSESLTGVIAAFTNPNGFWYNFVFAILIILFTYFY
                     TAITINPNQMAEDLKKNGGFIPGIKPGKRTAEFIDTVMSRITLPGSMFLALVAIMPAI
                     VSLFGVNSQFAQFYGGTSLLILVGVVLDTLQQVESHLLMRHYDGLTKSGRIKGRTSTG
                     MGGI"
     gene            complement(20801..21247)
                     /gene="rplO"
                     /locus_tag="PKY63_01625"
     CDS             complement(20801..21247)
                     /gene="rplO"
                     /locus_tag="PKY63_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPE99340.1"
                     /translation="MDLSNLKPAAGATKREKRIARGQGSGHGGTSTRGHKGAQSRSGY
                     SRKIGFEGGQMPLQRRVPKFGFKNINRVEYRALNIDMLQELAQQNNLQEITPEVLRAH
                     GLISKNDIVKVLGRGKLTLKLDVKAHAFSTAAVKAIEEQGGQAIKI"
     gene            complement(21267..21446)
                     /gene="rpmD"
                     /locus_tag="PKY63_01630"
     CDS             complement(21267..21446)
                     /gene="rpmD"
                     /locus_tag="PKY63_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPE99341.1"
                     /translation="MKKVRITLIQSLIDRPAIQRKNVQALGLGKMHSSVEHVATPQIL
                     GMIRKVNHLVKVEEI"
     gene            complement(21458..21967)
                     /gene="rpsE"
                     /locus_tag="PKY63_01635"
     CDS             complement(21458..21967)
                     /gene="rpsE"
                     /locus_tag="PKY63_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPE99342.1"
                     /translation="MSINRVKSSDIEFKDRLVSIQRVTKVTKGGRTFSFSAIVVVGNE
                     DGVVGYGLGKAKEVTSAISKGIEDAKKNLVRVPILKGTLPHQQEGKFGGATVFLKPAA
                     PGTGVIAGGAMRAVLESVGVKNVLAKSKGSSNPHNVVKATVDALMNLKDPRMIAQLRG
                     VSIDKVFNG"
     gene            complement(21982..22344)
                     /gene="rplR"
                     /locus_tag="PKY63_01640"
     CDS             complement(21982..22344)
                     /gene="rplR"
                     /locus_tag="PKY63_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPE99343.1"
                     /translation="MAIKSRKEFSRKRIKMRIRKVVSGTGECPRLSVYRSCKEIYAQI
                     IDDKSGKTLASASSRVKGVAEQKVTKTDKAKLVGKLVAERAIEAGVQSVVFDRNGYQY
                     HGRVKALADAAREGGLKF"
     gene            complement(22359..22916)
                     /gene="rplF"
                     /locus_tag="PKY63_01645"
     CDS             complement(22359..22916)
                     /gene="rplF"
                     /locus_tag="PKY63_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPE99344.1"
                     /translation="MSRIGKKQIDIPSGVQVNVAADNIVTVKGPKGELKQQVAPELKI
                     NIEGSVLHLNRSNDERQVRSFHGLYRSLLANMVKGVSEGFTTTMELVGVGYKCQANGQ
                     MLDLSLGHSHNILFELPKEVQIETITEKGKNPTIILKSHDKQLIGQVAAKIRSFRKPE
                     PYKGKGVRFQNEVLRRKAGKAAAAK"
     gene            complement(22938..23336)
                     /gene="rpsH"
                     /locus_tag="PKY63_01650"
     CDS             complement(22938..23336)
                     /gene="rpsH"
                     /locus_tag="PKY63_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPE99345.1"
                     /translation="MVTDSIADFLTRVRNAIKANHRIVEVPSNKMLVELAKLLHENGY
                     ILNYKVEESTMPRTLKVALKYNPINKLSAITEIKRVSRPGLRKYSPSDELPKVMNGLG
                     IAIISTSKGLMTDKQARKENVGGEVICFVS"
     gene            complement(23355..23624)
                     /gene="rpsN"
                     /locus_tag="PKY63_01655"
     CDS             complement(23355..23624)
                     /gene="rpsN"
                     /locus_tag="PKY63_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPE99346.1"
                     /translation="MAKESMKAREVKRLQMVEKYAAKKEALKAAGDFTALQRIPKNAS
                     KIRLHNRCKLTGRPKGYMRMFGISRINFRQMALNGEIPGVKKASW"
     gene            complement(23628..24179)
                     /gene="rplE"
                     /locus_tag="PKY63_01660"
     CDS             complement(23628..24179)
                     /gene="rplE"
                     /locus_tag="PKY63_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPE99347.1"
                     /translation="MAYIPRLREKYDKEIVPALMQEFQFKTVMQVPKLLKISVNQGVG
                     GAVTDKKLIDFAVDEMTNITGQKAVQTKSRKDISNFKVRKGMPVGVRVTLRGNNMYEF
                     LDRLISVSIPRVRDFRGISDKGFDGRGNFTFGVTEQIIFPEINIDKINKITGMDITFV
                     TSAANDKEAHALLKSFGIPFKKH"
     gene            complement(24179..24499)
                     /gene="rplX"
                     /locus_tag="PKY63_01665"
     CDS             complement(24179..24499)
                     /gene="rplX"
                     /locus_tag="PKY63_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPE99348.1"
                     /translation="MKVKIKIKKGDTVKVISGNDRGTEGRVLEVFPDSNTAIVEGVKI
                     VSKHSRPSAKDSKGGIVKKEAPINISNLMLVDKSGKPTRVGRQVKEDGTIVRYSKKSK
                     EEIK"
     gene            complement(24511..24879)
                     /gene="rplN"
                     /locus_tag="PKY63_01670"
     CDS             complement(24511..24879)
                     /gene="rplN"
                     /locus_tag="PKY63_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPE99349.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGGTKRRYASVGDKIIVSVK
                     DAIPSGNIKKGTVSKAVVVRTKKEIRRPDGSYIRFDDNAVVLLTASDELRGTRIFGPV
                     PRELRDKQYMKIVSLAPEVL"
     gene            complement(24882..25136)
                     /gene="rpsQ"
                     /locus_tag="PKY63_01675"
     CDS             complement(24882..25136)
                     /gene="rpsQ"
                     /locus_tag="PKY63_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPE99350.1"
                     /translation="MERNLRKERIGVVTSNKMDKSAVVLVERRLMHPKYGKFIKKTTK
                     FVAHDEKNECTIGDKVRIMETRPLSKTKRWRLVEIIEKAK"
     gene            complement(25149..25346)
                     /gene="rpmC"
                     /locus_tag="PKY63_01680"
     CDS             complement(25149..25346)
                     /gene="rpmC"
                     /locus_tag="PKY63_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPE99351.1"
                     /translation="MKSEVIKEMTTEELKERLTEEKKQVAKLQMNHAVNPLDNPGVIG
                     QTKKTIARIQTELRKRELEIK"
     gene            complement(25368..25790)
                     /gene="rplP"
                     /locus_tag="PKY63_01685"
     CDS             complement(25368..25790)
                     /gene="rplP"
                     /locus_tag="PKY63_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPE99352.1"
                     /translation="MLQPKKTKFRRVQKDKMKGNAKRGHQLAFGSFGLKTMEEAWITG
                     RQIEAARQALTRYMKREGQIWIRIFPDKPVTKKPAEVRMGKGKGAPEYFVAPITPGRI
                     MFEAEGVSLEVAKEALRLAAQKLPVKTKFIVRRDYSEA"
     gene            complement(25803..26573)
                     /gene="rpsC"
                     /locus_tag="PKY63_01690"
     CDS             complement(25803..26573)
                     /gene="rpsC"
                     /locus_tag="PKY63_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPE99353.1"
                     /translation="MGQKTNPIGNRLGITRGWDSIWYGGKTFGEKLVEDDKVRKYLNV
                     RLAKANVSKIFIERSVKLVTVTIHTAKPGFIIGQKGKDVDELREELKKITQKEVQINI
                     SEIKRPEVEAVLVANNIARQIEGRMSHRKAIKTAVASTMRMGAEGMKVTISGRIGGAE
                     MSRTEHYKEGRTPLHTLRADIDYARSEAHTTYGRLGIKVWICKGEMFGKPDFYGIDTP
                     KTDKKQRRGDRPDRGSDRGGQRPGGFNKPRGKKNNSNQ"
     gene            complement(26582..27010)
                     /gene="rplV"
                     /locus_tag="PKY63_01695"
     CDS             complement(26582..27010)
                     /gene="rplV"
                     /locus_tag="PKY63_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPE99354.1"
                     /translation="MGARKRISADKRKEARKEQFTAKLTGCPITPRKMRPVANLVRGK
                     NADIALAILKTSSNSSSDYLYKLLTSAINNWQQKTENKIRMEEAGLYVKSINVDGGRM
                     LKRIQPAPQGRAHRIKKRSNHVTIELGSRTEEVTNVETQE"
     gene            complement(27030..27299)
                     /gene="rpsS"
                     /locus_tag="PKY63_01700"
     CDS             complement(27030..27299)
                     /gene="rpsS"
                     /locus_tag="PKY63_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPE99355.1"
                     /translation="MSRSLKKGPYVHHKLEKRVIEAQKSSKKVTINTWSRASVITPDF
                     VGATIGVHNGNKFIPVYVTENMVGHKLGEFSPTRIFRGHAGQNKK"
     gene            complement(27312..28133)
                     /gene="rplB"
                     /locus_tag="PKY63_01705"
     CDS             complement(27312..28133)
                     /gene="rplB"
                     /locus_tag="PKY63_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPE99356.1"
                     /translation="MGLKKFKPTTPGQRFKLVSSFEEITAAAPEKSLVVPNRKSGGRN
                     NTGKRTMRYIGGGHKKMYRLIDFKREKEGIPATVKTIEYDPNRSARIALLFYKDGEKR
                     YIIAPQGLKVGQEIFSGKGVAPEIGNTMYLSEIPFGTVIHNIELNPGQGGKLARSAGS
                     YAQLLSRDGKYAVVKMPSGETRLILQSCRATVGTVSNAEHNLEVSGKAGRSRWQGRRP
                     RNRGVAMNPVDHPMGGGEGRQSGGHPRSRKGIPAKGYKTRRKKASDNLIIERRKK"
     gene            complement(28149..28439)
                     /gene="rplW"
                     /locus_tag="PKY63_01710"
     CDS             complement(28149..28439)
                     /gene="rplW"
                     /locus_tag="PKY63_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPE99357.1"
                     /translation="MDVLIRPLVTEKMTAKNTELNQFGFIVAKRANKIQIKEAVESLY
                     SVKVLEVNTMVYAGKRKFRGTRRGYVQGRTNAFKKAIVTLKEGEKIDFYSNV"
     gene            complement(28473..29111)
                     /gene="rplD"
                     /locus_tag="PKY63_01715"
     CDS             complement(28473..29111)
                     /gene="rplD"
                     /locus_tag="PKY63_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPE99358.1"
                     /translation="MEVKVYNTNGKESSKTVVLKDEIFAIQPNDHAIYLDVKRIQADK
                     RQGTADSTERSTLSGSTRKLFRQKGTGGARRGDIKSPLLHGGARVFGPHPRDYNFKVN
                     KKVQQLARKSALSYKVNEGAIRVIENFDFEAPKTSQMLEILKNFETSEKKSLIVLDES
                     KKNVYLSSRNLKKVKVINASKLNTYDILNAQIVLISEASLPQLDTIFLGEGE"
     gene            complement(29102..29731)
                     /gene="rplC"
                     /locus_tag="PKY63_01720"
     CDS             complement(29102..29731)
                     /gene="rplC"
                     /locus_tag="PKY63_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPE99359.1"
                     /translation="MSGLIGKKIGMTSFHEASGKIVPCTVIEAGPCVVTQVKTKQTDG
                     YDAIQLAYDERKEKNTSSAMKGHFKKAGTTPKKILMEFSRFEEGHQKNLGELVKADVF
                     IEGEYIDVVGFSKGKGFQGVVKRHGFKGVNQQTHGQHNRGRAPGSIGASSYPSRVFKG
                     MRMAGHDGNGRVKMINLQIMKIMPEQNLIIIKGSVPGPNGSYVILERWK"
     gene            complement(29754..30062)
                     /gene="rpsJ"
                     /locus_tag="PKY63_01725"
     CDS             complement(29754..30062)
                     /gene="rpsJ"
                     /locus_tag="PKY63_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPE99360.1"
                     /translation="MSQKIRIKLKSYDHYLVDKSAEKIVKIVKATNAVVNGPIPLPTD
                     KKFFTVNRSTFVNKKAREQFQLFTYKRLIDIYYNQQSKTIDELMKLELPRGVEIEIKV
                     "
     gene            complement(30093..32192)
                     /gene="fusA"
                     /locus_tag="PKY63_01730"
     CDS             complement(30093..32192)
                     /gene="fusA"
                     /locus_tag="PKY63_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPE99361.1"
                     /translation="MTDLNQIRNIGIMAHIDAGKTTTTERILYYSGINYEIGEVDDGA
                     ATMDWMIQEQERGITITSAATTVYWVSNNEKYQVNVIDTPGHVDFTVEVERSLRVLDG
                     AIAIFCAVGAVQPQSETVWKQANHYRVPRICYVNKMDRQGADFLRVVAQIREKLRANP
                     IVVQLPIGVEDSFIGVVDLIENKAFVWDDDSLGKEFSEIPVPDDMLSIVEEYRLKLIE
                     GAAEEDPELLERYMDNPDSISANDIRKQLRKATLESRAFPVFCGSSFKNKGVQTLLDG
                     VIHYLPSPVEMEEIEGINPKTEAKESRSNSVNAPFCALVFKIANDPYVGRLAFTRVYS
                     GSLQSGVMVYNSNTEQKERVSRLIRMHSNKQNPLESVEAGDICAIIGLRNIHTGDTLC
                     DEKQPIVLESMDFPEPVVSIAIEPKTQNDLDKLNASLQKIAEEDPTFTIRQDDETGQT
                     IISGMGELHLDIIVDRLKRESKVECTLGKPQVAYREAVGGTITHRETYRKQASGRGRF
                     AEIEIVVSPAPIDQKGLQFVNSTKASVLSKEFVAAVEKGIKAAMGTGVMAGYPVYCAK
                     VELVDAVMSSESDALSFEICGTIAFKEATRKIRCNLLEPIMKLEVDTPEEYVGDVTGD
                     INRRRGEITGIEAQVYSTAVKAIIPLGETFGYITSLRSLTSGRANMSMEFSHYSITPN
                     ELAQDIIFKMKGIRVTL"
     gene            complement(32201..32668)
                     /gene="rpsG"
                     /locus_tag="PKY63_01735"
     CDS             complement(32201..32668)
                     /gene="rpsG"
                     /locus_tag="PKY63_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPE99362.1"
                     /translation="MRKSKPKKRIILPDPKFGDQLVTKFVNNLMYSGKKNASYQIFYD
                     AIDQIADKSKENGLEVFKKALENVTPSVEVRSRRIGGATFQIPSEIRPERKSSIGMKN
                     LIAFARKRHEKSMASKLAAEVLAAYKEEGSAYKRKEDIHRMAEANKAFSHFRF"
     gene            complement(32689..33069)
                     /gene="rpsL"
                     /locus_tag="PKY63_01740"
     CDS             complement(32689..33069)
                     /gene="rpsL"
                     /locus_tag="PKY63_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPE99363.1"
                     /translation="MPTIQQLVRKGRKELEYKSKSPALASCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNQFEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DTAGVEGRLQRRSKYGAKRPKKKA"
     gene            complement(33361..38415)
                     /locus_tag="PKY63_01745"
     CDS             complement(33361..38415)
                     /locus_tag="PKY63_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPE99364.1"
                     /translation="MNKNLLFLFFLIGLFCCNDVKAQNYGNEWINYSQPYFKIKVAQD
                     GIYRISYSTLASSGVPIASFDPRQFQLFAFGEEQYIYVHGENDGFFNSGDYIEFYGKR
                     NRGDFDADVYDNPADMPNPDYSLFNDTTIYFLTWNSSITNRRVSIETDRNFSGYTSES
                     YVWFNSRTNYTARYFNGKTDQNGITDPRYTNCEGWFDTGFDINSSLIYTRSVPTPSVF
                     ASGPDASVDLMLLGASDYINAYPDHHVMMNFAGLTIDTIFEGYVPVKIVRSRPAGSFT
                     SGSTVFNFTLPDDLGSVADFTTLSYIDIRYPRQLNLNSSGSLLFESNDGAGAKTYFAF
                     TGLTAPTTDTVVVHDLTNHRRILTSRAGASVNFLVPNSGGLKQMYLAAESNVVYVSSM
                     QPVNSSGTSGYFINYGDAAYSGTDYLIVTHRSLLAKANNYASYRSTTGYNPLVADVDL
                     LYDEFAYGINKHPLGITNFTRFARAYFADTIHGLFLIGKGYRAGDTQYNYRKNVTYNG
                     LTLVPSFGNPPSDNLFTSELGDNNYAQHIPTGRLAARNSTAVEWYLEKMQDFETEQNQ
                     PFNPANPFANQWMKRVLHFAGGSDYGQMMTLLNYLNHYRDTLQAPYFGADVKTFSKSS
                     TAPIQQNQSDSLKDLINEGVAILNFFGHAAGIGFDISIDNPQDYSNYKRYPFLIANSC
                     FAGDLYQPTNSSAEEFVLIENKGTIGYLGSITKSLMTSLDSYSSELIRRISKQDYNKP
                     IGYIMMETVKTLQSYTTSSYIRDVCLEMTLHGDPVMRIGGQPQPDLMLTTGSIYFNPS
                     NVTSDLDSFAVNVITHNVGMAVSDSVILELTRIYPDNTTEVYQKMIKSPAFADTIVFM
                     LPVSPLTGVGLNSFSAMVDAFDALSEANETNNSTNTTLFIISNDVNPVYPYEFEVLPD
                     TFVTLVASTGYAMASTGTYVFQIDTTDSFDSPFLVQSNPITQSGGIIEWALPFSMLSM
                     PDSTVYFWRISSTSNSNWRESSFQYITGKRGWGQAHFFQFKKDNFNYVSYNKPSRILE
                     FINTIVNIQAQTGYYPYILWSEEWYKINNVLKGQWSCTNYNGDGMKFAIFDTLSISPM
                     ANTDPDLDGLGPFDALNCRSYVYYDFDFFTYGEPWYSRMYNMIDSVPDGYYVLAFSHR
                     NHNAQNWPDSLYEAFESIGSNVIRSLPNNVPYIIFGRKGYLNMADERVGAGLTSIISG
                     SWDITTNWKEGYIESTTIGPAAEWGSLHWRVKSFEPGIWTDTVRLSVIGIKQDGTEDV
                     VIADLPPIPDSLDILNLSSRIDASVYPYLRLHMNMSDDSLRTPAQLERWQVLFDPVPE
                     TAIDPVSYYTFYNDTLQEGEMVNLAIATRNVGPVDFPDSLSVAYWLIDNERNIHEIAW
                     KKLRLHPVGDILVDSISFSSEGFAGSNSLWVEFNPVDSATGFYDQMEQYHFNNIAEIK
                     FTVGKDLINPMLDVTFDGIHILDGDIVSAQPLIEILLKDENKYLMLDDTSAFRIYLLK
                     PGATDLERVFFTEDGQENMRFYSSSSTLNNVARIEFPAGAFSDGVYKLVVQARDKSGN
                     ESGSIDYSINFEVINKPAITDVLNWPNPFSTKTHFVFTLTGSVVPEYFKIQVMTVTGK
                     VVREIDKSELGPIHIGRNITEYAWDGRDEFGDQLANGVYLYRVIVKLDGLQMEKIETP
                     AGQYFTKEFGKMMLIR"
     gene            complement(38419..39519)
                     /locus_tag="PKY63_01750"
     CDS             complement(38419..39519)
                     /locus_tag="PKY63_01750"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPE99365.1"
                     /translation="MNKFIAVLVLFFLVISVFAQAPKYSNEFLAIGVGGRALGMSNSV
                     VASVGDASAGYWNPAGLVDCEAPRQLFLMHSEYFAGIAKYDNISLAAVTSDSSALAFS
                     FIRFGVDDIPNTTELIDAQGNIDYDRITTFSAIDNAFIVSYSRKLPIKNLQVGGNLKI
                     IRRTVGDFGGSWGFGIDAGAQYRKDNWRFGAVLRDVTSTFNAWSFTLDDATREVFTLT
                     GNEIPENSLELTMPRLLLGAGRKFDISNSFSIYPEAGMDITFDGKRNVILKTGFASAD
                     LHAGFELNYKNVAYLRGGFGNIQQETNFDGEKVTTVQPNMGVGIRIKDIMSIDYALTD
                     LGDVSVALYSHVFSLVFNIRPKGTGSSINPSI"
     gene            complement(39583..40134)
                     /locus_tag="PKY63_01755"
     CDS             complement(39583..40134)
                     /locus_tag="PKY63_01755"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE99366.1"
                     /translation="MEGKIYTKKGDSGTTSLIGGKNVSKSCIRVESYGMVDELNSFTG
                     CLYEKVEAGDTRNFLFEVMNRLFLIESHLAVDPVSGSKKYFPELLASDTEAIEKEIDR
                     LTAALPPLANFILPAGCEKAAMAHICRTVCRRAERTILRLYETEPVHPEILKYINRLS
                     DYFFVLARSLAHSCGSGDVVWKI"
     gene            complement(40149..40883)
                     /locus_tag="PKY63_01760"
     CDS             complement(40149..40883)
                     /locus_tag="PKY63_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE99367.1"
                     /translation="MPEVIRLENISRHYKVGTVLVKALNSVSFNIERGEYVALMGPSG
                     SGKSTLMNILGCLDTPTGGTYILNNQDVSQLDDNQLAEIRNKEIGFVFQTFNLLPRYT
                     ALDNVALPLIYAGIRKEARMERAEKSLTDVGLSDRMEHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDTRTSVEIMGLLDELHKTGNTIIVVTHEEDIARYAHRIVRLRD
                     GLVETDEKNPKVTSYQEVLESLKEHE"
     gene            complement(40951..41721)
                     /locus_tag="PKY63_01765"
     CDS             complement(40951..41721)
                     /locus_tag="PKY63_01765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPE99368.1"
                     /translation="MSRIIAAMRHFFQARGLRSVHSPWLYEIYHSLCYPKALKESAIT
                     KLAFLHGQLSVDKTPLVFNELGSREGRLETTVFEVFKRTSTSIGEAEMIAELAAHVNG
                     KTILEMGTAFGTTTLAMHFAAHKSRIITLEGVPEIAAIAKEHFEKYEAENVILKTGRF
                     SETMPQIVAEEKDLGLVFIDGHHCRKPTLEYLEMILPALSEKAVVVIDDIYYSREMSQ
                     CWSELQQHPAFQVKMDFFRFGLLIKNADLSVECFRLRV"
     gene            complement(41731..42783)
                     /locus_tag="PKY63_01770"
     CDS             complement(41731..42783)
                     /locus_tag="PKY63_01770"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPE99369.1"
                     /translation="MSAPGLKMIVAGPCSAESEKLCLDVAAGLARTGYVDLFRGGVWK
                     PRTLRGMYEGPGNDGLKYLLAVRDQYKLPVVTEAATTAHVEAVLKAGLDAIWVGARTV
                     TDPFSVQELANALQGSNIRILVKNPLVPDVRLWAGAVDRLLNKGLNVEALIHRGFVTY
                     NESKFRNEPLWVLMAEMRTRYPEIKIIADPSHMAGNSFYVAGLAAQAKEMEADGLMIE
                     VHADPPSALSDANQQITPAEFVFLIDSLCGKQSTSLHDASLEALRREIDETDERLLRI
                     LAQRIDIVKRMGIVKRDAGMTIMQGGRWKSLVEDRLKRASALDLNDEFIRELLQVIHD
                     ESLRIQLSILDKDENS"
     gene            complement(42912..45899)
                     /locus_tag="PKY63_01775"
     CDS             complement(42912..45899)
                     /locus_tag="PKY63_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPE99370.1"
                     /translation="MTKQHNKPDLNQAQFQDTLFEHLMERRIEHILLICSTYDAFILE
                     EDGRINEAIFQEYVSLNLRYPPKFFKAHSAEHALEILENDRVDLVINMLSISDQNPFE
                     FSKVIKQRFANIPVVVLTPFSREVSQRLAVQDLSGVDYVFSWLGNTNLLLAIIKLIED
                     KMNTDFDVGEVGVQCILLVEDSVRYYSSYLPNMYRIVIQQSRAFMSEGLNEHQRTLRL
                     RGRPKILLATNFEQALSLYEKYKSNMLGMISDISFNRNNIKDKNAGIELSRIVQADDP
                     YFPVLLQSSDVITQDLINDIKVKFVHKFSKTLLHELTDFLLDYLAFGDFRIVNPANGE
                     EVARARDLQSLQEQIYSIPDESLKYHIDRNHFSKWLRARALFSLGKLFRQANSKDFAS
                     LDEVRDYLYKGMAKYRGVRGRGVIATFDRNKYNEYILFSRVGEGSIGGKARGLAFIDN
                     ILKRNQLAHRFGSVELTIPRTVVLATDVFSEFMERNDLYDFAIASGNDEEILSRFLEA
                     ELNYETILDLKSILRVMKKPLAVRSSSLLEDSHYQPFAGIYSTYMIPNTGDDRDRLKM
                     LMQCIKAVYASVYYQESKAYMTSTSNLIDEEKMAIILQEVCGLNEGDYYFPVLSGVAR
                     SVNFYPISPEKAEDGVVSIAFGLGKYIVDGGMSLRFSPRFPEKAIQLSSTEMMLKDTQ
                     KEFFAINLKRNLFTPKVDDNAHIERFSVSDGDQFKTFRMAASTYVYHDDRVVDGISQK
                     GMRIITFNHFLKHNVFPLAEMMTDILETSSREMGKPVEIEFAVNMDGLGHHKVFNLLQ
                     IRPIVDNKENLTINPEHIRHDACIIISESALGNGVYDNICDVVYVVPDKFNAADTLHL
                     AQVIDQLNKSLSNEKRNYLLIGPGRWGSSDPWLGIPVKWSQISAARVIVEAGMNHFHI
                     EPSQGTHFFQNLTSFRVAYFTVNDFIKQGMYNTAFLNAQEALFDNGVIRHVRFDQPME
                     ILVDGKKHLGIVSMPGMKAISGI"
     gene            46101..46811
                     /gene="radC"
                     /locus_tag="PKY63_01780"
     CDS             46101..46811
                     /gene="radC"
                     /locus_tag="PKY63_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPE99371.1"
                     /translation="MEKNKKKSIKEWAADDRPREKMLAKGPEALSNAELFAILIGSGT
                     TKKSALELAREMLENADNSLKKLSTLTIHEIKKIHGIGPAKAVTVAAALEIARRREGE
                     SIDIEELVLSSRDAQKALLPMLCDKQHEEFCIMGLNRRNKIIFKERISMGSMVATQVD
                     IRKIIKMALDTRSLSLILGHNHPSGNLNPSDSDINLTRQIYEASALFDIRLLDHIIIS
                     QDSYYSFMDEGILNTFKK"
     gene            46808..47974
                     /gene="wecB"
                     /locus_tag="PKY63_01785"
     CDS             46808..47974
                     /gene="wecB"
                     /locus_tag="PKY63_01785"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPE99372.1"
                     /translation="MKIVTIVGARPQIIKAAALSREIQNHFNDEIKEFIIHTGQHYDD
                     NMSEVFFRELSIPAPNVNLNIGSSSHGEQTGRMMEGIEKVLTENKPDALVIYGDTNST
                     LAGAVAAAKMHIPVFHIEAGLRSFNKKMPEEINRIVADHCSTLMFCPTKTAIQNLENE
                     GFPAGLMSPYSMDKPGVFHAGDIMFDNSVFFAQIAEKQSKIIEDQNLKDIPFILATVH
                     RNDNTDNEDRLRMIFDALCLASINTKLPVVFPLHPRTKKMMDAWNIGSNIPAGADVRI
                     IPPASFLDMILLEKNCRLVLTDSGGVQKEAYFFSRPSIILRPETEWVEIVDLRAAKLA
                     DHSAPEIAAQAEAMINYPPTEFPPVFGDGHAAEFICEKMLEFYQSLLRKNAIKK"
     gene            47974..49275
                     /locus_tag="PKY63_01790"
     CDS             47974..49275
                     /locus_tag="PKY63_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE99373.1"
                     /translation="MSITILTSFDNAKLRYTLDYIFDERLGCGYVLNPENIKNNTAIV
                     SYGTNHNQGLLISDSNFLHPKGVSAQKAEVLIDDKNNICLFPAEESADFSFDVFAAIF
                     FMLSRYEEYTSLERDQHGRFDLQHSLAYKNNFHLRAVVDEWIIILRSRLLKRFSSVTF
                     KKESPSFAITVDVDMLFSYRTKGFLRNMAGWARDLVKGKFQALFERPLVLLGVRKDPF
                     DTFDIIDKISREFHVPILFFVLASQQRTNFDKNGNLHHQRAVKQLKKISRESLIGLHP
                     SYYCLDDDNKLIKEKQNLEKTIGTSVTAARRHFLRMNLPQSYRQLIKAGITNDYTMGF
                     ASGHGFRAGTTRPFRFFDIESDIVLPLIVHPFCIMDGSLKDYQQMTQEESHREMLHLA
                     EYIQSINGHFEMLLHNETLSESGRWKGWTQIIKETIRQFQS"
     gene            49281..50216
                     /locus_tag="PKY63_01795"
     CDS             49281..50216
                     /locus_tag="PKY63_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99374.1"
                     /translation="MIRFIKNESISRDLWDKAIMQSPQPMMYAMSWYLDLVTPGWDGL
                     IEDNYQAVMPLIGAKKFGIHYLYQPPFCQQLGVFGKNTSTDVVKSFLLAIPRKYRFAE
                     IMMNESNKIDLTNAEMLTNITLQLGCAIENIRTGYNENTRRNIRKAEQNKLSVYKGFD
                     IENIISLFKTNKGEALGKNESWYSTLKTLSYQLRHLSLANTFCAMNQHNEIIAGILLV
                     EFNGRAVLLFSGSGSEARETGAMHFLVDSILKDICTSHQIFDFEGSNNENLAKFYLGF
                     GGKKSSYPFVRINRLPFPVRWMKGKGLDHSQISAK"
     gene            50213..50725
                     /locus_tag="PKY63_01800"
     CDS             50213..50725
                     /locus_tag="PKY63_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99375.1"
                     /translation="MKLLDVFDKDPDNQTGCSNILMKCLFSFVITYLILLIFSLVSNN
                     KESQQWLPIILLIVNPIICIINSIYMFLLFALGFDKGGISGMLFILIESIFLIAIYSG
                     ISLVVTKLSVNVLYEIENGSMLRRNVFSEHFQLLFTYFLLTIFLVALWKILKKLPECR
                     CQSIALLLSS"
     gene            50958..51191
                     /locus_tag="PKY63_01805"
     CDS             50958..51191
                     /locus_tag="PKY63_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99376.1"
                     /translation="MKTLSLKLDENIFLETEKVLSEIKKPRNRYINEALKFYNALQKR
                     RLLSKKLKAESGLVAEESMNILYEFENFEDDED"
     gene            51181..51531
                     /locus_tag="PKY63_01810"
     CDS             51181..51531
                     /locus_tag="PKY63_01810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPE99377.1"
                     /translation="MKIKQFDIWIANLDPRFGTEAGKSRPVLIVQSNLLNDIHPSTLI
                     CPITTNVQPKAEILRVHLKKGHFGLKQNCDVMIDQIRAIDNKRLMKRVGRISDSLIEK
                     VKENSAIVLDLTKT"
     gene            complement(51598..53760)
                     /locus_tag="PKY63_01815"
     CDS             complement(51598..53760)
                     /locus_tag="PKY63_01815"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPE99378.1"
                     /translation="MKNRIKIAGVLLFVSLFFQSRADEGMWLPMFIDRLNYTDMQKMG
                     LHLTPEEIYSVNNGSLKDAIVIFGGGCTGEIVSDQGLIFTNHHCGYASIASLSTVDND
                     ILTNGFYASTMADELFPASNLNVRFLARIEDVTKQILDSIPYEATEMERDEIVGRISK
                     RLEKNAVSESYQEANVKPFFYGNEYYLFVYEVYSDVRLVLNPPSSIGNFGGDTDNWMW
                     PRHTGDFSVFRVYTGPDGKPAKYSKNNIPMKPKHFLPVSIKPKTENDFAMVFGYPGRT
                     SRYITSEEVASNYLYVNPSIVKIREKKLALMDANMKASDEVRLRYASKYATTANYWKY
                     YIGQNKGIERNNVIERKKILEDDFETWVNNDDIPKFGYYKNALIKTHNSIEKLSSLRK
                     VQYYISEAFFRGSDVISFAGKFRSLMAELSSDKPNLTKIDDMVSYLKRVSENHFVTFD
                     FKTEMQLFSAMTAMYAADVPAEYHPAFFSIISNKFKNDYALYSDFAFLVSVFSNKDKM
                     DAFLENPTKDVLLKDPLYEAAIQVYEIYNKLVGEINPLNYQLNQGMRLYVQGLREMHP
                     EKHFYPDANSTLRLTYGKVLSYSASDAIDYDYVTTLNGVMEKENPDNDEFIVPARLKE
                     LYEKKDFGQWGENGKMITCFLTNNDITGGNSGSPVLNGNGELIGLAFDANWEAMSGDI
                     VFEPELQRCICVDIRYVLFYIDKYAGGTRLIEELEIRK"
     gene            complement(53980..57387)
                     /locus_tag="PKY63_01820"
     CDS             complement(53980..57387)
                     /locus_tag="PKY63_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPE99379.1"
                     /translation="MKTVIFSFLMMVLMGSMSSQTYHSLNNDAGKPLYTVISSTPDET
                     VIEYNFQGFYMSDVQTSQGVKQKVSMDGASPLLTAGEPALCRFSNSLIVPDAADMKVK
                     VLSADYVDFSNVEIIPSKGSLSRDIDPSSVAYTWGRTYQQNGSFPGAFASLSDPYIMR
                     DYRGVALHVYPFRYDHAVRSLRVVKTIRIVVYKDSDNSALNAFNRTKTLSKVDESFDE
                     LYNRHFINYSQVKYTPITERGRILVVCKPEYMANMAPYVQWKNRIGFPTEIVSTTTTG
                     TTSTAIKTYVTNYYNTNGLTFLLFVGDGGDIPPITSGVAGHSDNAYAYQTGSDHYPEF
                     YVGRFSAESTGDVDVQVLRTLTYEQNPNTSNNWLNRVLGIASSQGPGDEGEYDYEHIR
                     NLQTQCDNYEYETKYELFDGSQGGLDAAGDPTAANVSTVLNAGAGLLLYTGHGSDISF
                     STTDFNNTDVGNLTNTTQWPFIISVACVNGNFVGQTCFAEAWLRARNGTTPKGAVATI
                     MSTINQDWAPPMEGQDEMVNVLCETSAGNIKHTFGGIVMSGCMKMNDSYGSDGYEMTD
                     TWTIFGDPSLMIRTDTPKTMTVTHLATVPIGTAQLVVNCNTEDAFVTVTLNDGIIGTG
                     IVSGGVATISFTALSSIDTLVVTATKYNYFPYTGEVPVIAATGPYVIYSTFSINDASE
                     NNNSQADFGEDILLNVTLNNVGVANAANVIATISTTNPAINITDNTETYGAINAGATK
                     PMNNAFAVSVGSDVVDQTSVLFGLTTVDGADTWNSSFGMTINAPSLSIGGLVIDDAAG
                     NNNGKLDPGETVNILIASLNNGHAMYPAATGVLSSVSSGISIVNGTDNIGDMIPALTH
                     DAGFSISVDPGATLGSAVSLTYTLGTGFYQVEKVFTMKVGIVDEDYETGDLTQYAWTT
                     GGNAPWFVSTNNPYEGVYCSQSGDIANEQTSTMSVEWDVASNDTISFYYKVSCENGSL
                     YSQKWDYLEFLIDASSKDWWDGEQGWSRAAYPVTSGQHTFTWKYLKDYTAADGDDAAW
                     VDYIVFPALANHTGLDEPTFSFNVYPNPAVESMFVDIELGTADNVSIELLDNTGRLLE
                     VLFSGTMPKGFSRTAIDAGVLSGGVYYIRVKTSEGSISRMIMTAGAE"
     gene            complement(57399..60848)
                     /locus_tag="PKY63_01825"
     CDS             complement(57399..60848)
                     /locus_tag="PKY63_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPE99380.1"
                     /translation="MKRSLVLILAIMLTYAGFGQSRVLDAEAVKVLNMSSAGLQIEVT
                     APQFEIAVIKTEAGMFNSMVVQGMVARTEAGMPFLPAISRLIQVPENAQVLVDIDYSH
                     YTDYDLSSISGNAYQLMPYQPGVSKSHTGEIPFYYNNAAYKINDFCQNERVAVDIHGN
                     LRNIRMASLDIHLFDYNPVSNTLRVYDKLILNISFPGGDAVLTNQLSEKYADVCFAGP
                     VARLINHEAFIHQRDTASHYPIKMIIVADTMFHDALQPFIAWKTKKGFIVDEAYTSNP
                     AVGTTTTSISNYLQAKYDNATTADPAPTFILIVGDVAQIPAFSGTAGSHYTDMYYAEF
                     TGDMIADAYFGRFSATNVDQLLPQIDKTLEYEQYLFPSESWLDTVVMIAGADASWAPT
                     HANGQINYGTTYYFNATNGIYSWTHLYPASASDDAIIRTEIGNGVALANYTAHGYSDG
                     WGDPSFDKTDIPSMNNAHQYPLMIGNACSTNTFYESECFGEALLRTPMKGAIGYIGGS
                     NSTYWDEDFYWGVGVRTTINANPVYDANNLGAYDRTWHITGEPFSEWFVSQGQMILAG
                     NLAVLEGSPSSFDYYSEIYHLMGDPSLMVYLSVPSQLSVTHPALVPLGQNTITVSTEP
                     YAYVGVSQNGVWHGAGMADATGNVVIEIIPFTTPGTADIVGTKQFRKPFVSSFVVQTP
                     SGPYVLMENKSIDDAAANNNQQADIMETVGLNVRLKNYGLQNDSTVYAILSSSDPYVE
                     IVDSIGLWGLIASNDTLTLNNAFTVKIDTLVPDQYMAPFQISIRDSSGHSWSSDFNLK
                     INAPLLQILNLTINDAVGGNGNNKLDAGETVAMNIQVVNNGHSHAWDASALLSSVSPY
                     LTITSAAWQHDSLFTGIAETGTYTVEVSSSAPMGTAASVTLNLSALSVYQASQTFNRS
                     IGQIDEDWESGDLTQYEWVTGGGAPWIITSGSPYEGSFAAQSGNITDNQQSDLSITID
                     VLSDDSLSFYKKVSSEQGWDFLKFFVNGTSMGEWSGELAWSQERFWITAGTKTLKWSY
                     IKDYSYYDGDDAAWIDYIVFPPMYVVTSDIELPSAENDLLVYPNPATNGFVTVTAPVI
                     SGDVQLTIVASDGRLVYSENYHNISGRLYLPVSVYEWEPGIYTVHLNGDQVNFSRSFA
                     VIK"
     gene            60996..61799
                     /locus_tag="PKY63_01830"
     CDS             60996..61799
                     /locus_tag="PKY63_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99381.1"
                     /translation="MRLSLLTILFFVSLASQAKPQNNNVDRLTINGYYFGKNLVVINP
                     LNGDRFAVESVEVNGNPTTDEINSSVFEIDFAAMALNQGDPVSVSINYYVGAENPIVF
                     NPEALEAASNFSFKSSFLEKKTEKIAWTIAGSPGTEMFEIEQYRWEKWVRVGTVNSKD
                     SISLNTYSCKIIPHYGKNLFRVKLVDPKGNITYSNPVKLQSKTPEVMLVKTTVETTIE
                     FTGETLYQIYDEKGIRLLNGSGSNVDVSSLMVGKYWVNYDNKTELIKKK"
     gene            61814..62584
                     /locus_tag="PKY63_01835"
     CDS             61814..62584
                     /locus_tag="PKY63_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99382.1"
                     /translation="MSNPGPAPIPLFTDRIPDSVLQSQVLARITNPALIDQGYTNLCG
                     MAVCAMTLAKYDAIGYRKIMTGLNDSNKAEYNQYKLKVPIRITKTDENFFRKGKLPEA
                     DWLLLATMRNKCNYILPYGGRMCKWYEKIAGSNFPSEVRRTLKKMGFEKISDNMNGFW
                     PIRKPALQTLQTLDSVFTQGHTPIILINTRMYHKGTFSVKSNHFVIYNGNLSFDNETD
                     KVTFNVWTFGYGAGKTITCSMEAFRNNYFGCIVVKKPG"
BASE COUNT        17660 a        14453 c        12714 g        17616 t
ORIGIN      
        1 tgaaaaaatt cctgcggttc aacaacaaca taaggaatat gtcccccagc acctttaaaa
       61 aaatcagttg attggctttc ttcaccaaat gcaagcatat aaaccggagg gaaattgttg
      121 tgattgtttt tcaactcaag aagcaatttt gaagattcct gacagtgttc gcaatcaaaa
      181 ctatataaac aaacaatctt tttgccctca ttcaggtcaa ccgctccggt attgaatttg
      241 gtgtactttg tgaaaatcga attcactttt tttcttcctt caataacatt ctgattttcg
      301 acaacctttt tcatttcagt aattatttca ttgccggatt cgtcaataac agtatcggca
      361 acaagaaccg aatcaacctc agcaacaata gtgtcagaga caatttcctg cgatgcctca
      421 acggtattgc aattgaattt tggtggttgc aaaatgagta ttacaagata aacaatcaga
      481 aaaataacag caggcaccaa aaaattatca ttcttctttt tctttacttt cagaaaaata
      541 tataccagca ttgccattgt aagaagattt ttaacgatag cctccaacgg tggcatagga
      601 atcaactctc cgaaacaccc gcagctaccc acgtttccgg aggtataaaa aacccacgac
      661 aaatgaatgc agaataccag gagcaacaaa aaagtgctcg gaacaattac atttcgcaga
      721 aaattatttt gcagaattgc aatgcccaga aaaatttcaa acccaacaat ggcccgggca
      781 agcaaaggcg caatgcacca atcggctatt cccagcccga caatttgttt ggcaaaaaca
      841 tgcaatgctg actctagtgg aaaaaacttc ccgtatgccg acaataaaaa cagtccgctc
      901 acaaggattc tgaatatcca cagaataatg tcctgattac tttttttcat ttctttaaat
      961 ctgatttatg ctttgaaatg tgaggcaaat tactgaactt ttcttaaaac aaagcttctg
     1021 atgacaggtg caataatcag tgcaaccggc aaagccgtga tataagaaac ggcccatgct
     1081 ttcatccaat gcaggaaaaa cccgcttgca aagccgaaat tcataagagt gcccatacca
     1141 gagactacca ttgtcatgat caggatcatt agagaaagaa agaataacat tggttttttt
     1201 gagttcataa aaagatcatt ggttttttac ttctttttcg acaggaaacc cattttcaat
     1261 ccagccggtc aggccatcca tcatacagat gatagtgccg ccatagctca gcttttgcag
     1321 catttccaca attgtttctg tacggttttt cgttgtgcaa tacactacga tggttccaaa
     1381 attaaggtat tgcgaaagca attcgctggc ttcagcagaa aaagcgtcga gacaaaccgc
     1441 atttttcaaa tgaccgttgc aaaatttagc actatcccgt ccatcaataa ttaccgaaga
     1501 tgcggaatcc aattcaggca gcatctgcat tacatcataa gacgtttttt gctgtaatgc
     1561 ctgcgatacc gcctgattgt aacaaacaaa cagcaacaaa caaaacaaag ctattttgat
     1621 tatattcatt cttaaaagaa aaatccgtaa atcaatcccg aaagtgttgc cattgcaatt
     1681 accagcagaa cgtaaaccac agttttctgc gttccgatta cgccacggat aacaagcata
     1741 ttaggcaacg acaaagatgg acccgctaac aaaagagcca gtgcagggcc tttccccatg
     1801 cctgaagcga ttaaaccctg cagaatcggc acttcggtga gggtggaaaa atacataaat
     1861 gcaccgacaa ttgaagcaaa gaaattggag aataccgagt ttccgccaac cagagaagaa
     1921 atccattcgt tgggaattac accggcaatg ctaacgtcgt cgtgggttga gcccaacaga
     1981 aagcccgctg taataacacc aactgcaagc agtggcaaaa tctgttttgc aaaaccccat
     2041 gtcgaatagg tccactcttt attgtcctga tcacgtttat cgaagagggt cataatgctg
     2101 agtgcagcaa ttccaatcag cataggaacc agcggagcca gttttgcatt ttcaataaat
     2161 tcaaatgcga gaaatccgga taaagctgtc agaatgccgc cggcaatcac ccacatccaa
     2221 tgtattttca gaatgcgaat gagcgaatac atcagcaaaa tggaaaagcc gcctgtaatc
     2281 caccatttgt aactccagat gtaaaaccat gtgctggaag tgtcatcagc agacggtttg
     2341 ccccagtttg caaaaacaag aatgagcaca agtacaaaga aatgaaacga agtttgccac
     2401 ataggtcgct tttcgggggg caactgaatg ttcatctgtt cttcgcgctt tagtttttct
     2461 tctttgcggt aaatgagcga catggatacg ccaataatca cactgaacaa gacagcccca
     2521 attgttcggg ccacgcccat ttcgaatcca agaatgcgag cagtgagaat aatggccagg
     2581 atattaatgg ccgggccgga atacaaaaaa gcgatggccg gccctagccc tgcgccacgt
     2641 ttgtggatac ttgaaaacaa aggtaaaatt gtgcaactgc acaccgccag aatagttccg
     2701 gatacagctg cgaccgtgta ggcaagccat ttttttgcat tcgcaccaaa gtatttcagc
     2761 acagatccct ggctgacaaa aacagcaatc acaccagcaa taaaaaacgc tggcagcaaa
     2821 cataaaacaa catgttctcg ggcataccat ttcaccagat ccaatgtagc tgcaatggcc
     2881 gtattaaaac gtgtattttc aattggtaaa aaaaatgccg ctgcaaaaac cacagcaatc
     2941 caaaacagga tttttaattc ttttttcttt tccatttcaa tcaattcaaa taatccataa
     3001 aacaatcacg aaatacagtc ctgctgcaat aaagaggaca gctgccacta gacgaaacca
     3061 cttctcgaaa agtttgattc tgttgtaaaa agcaccaaca cccgacatgc taaaagcaag
     3121 aagccaggaa atgagaatta caggaagtgc tgtcgccacg ccaaataccg gaggaagtaa
     3181 aagtccgtct ggactggcaa cagtcattgg aatgagcatg agaaaataaa acacaccgct
     3241 gctcgggcaa aatgccaatg caaacaaaac gcctaacata aacgcccacc aggagctgcc
     3301 tccggctgcc ttctgttcaa ctttttctga cagacttccg ccatttttcg aaatgcgtat
     3361 tttaattaca ttgagcatca gcacaccgat aacaatgagc aaaggcccca gaaatttttc
     3421 gccatactgg ctgaaaagtt ttgcaacttt gaatgtgctt gcgccgaaat aaatgatgat
     3481 gcccagccct gaatagctga ccagacgccc gaaagtgtac aacaatccgt tgtaaaagat
     3541 ttttctgcct gacgaaattt cacggctgat ataaccgata gctgttatgt tggttgccaa
     3601 cggacaggga ctgatggctg tcatcaaccc caatgcaaat gcagcaaata tgggatattg
     3661 cgcgttgtcg gcccaggttt gtaagaattc tatcatttga gagtttgtaa agtttttaaa
     3721 gtttataaag tggaaaggca ctttttgagt tatgagttag gagttatgag tgataagttt
     3781 gttagagtag ttagacaggc gtaaggcata agaacgttgt gcattgtgcg ttgtgcctta
     3841 ttgcagcaac atcatttcat caattttctc gcggagcttt tcgacaaaga gttctggctt
     3901 gccgattttc gaaaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     4021 gatgtgaaaa tcatacacca gcagttttgt gttttcgttc tgcgccagca tggatacaga
     4081 aaccagcagc aatgcggcaa atgacaagat cgatcttttc attttgaatt attttaggtc
     4141 tttctgaatt tgctccctga gtaatttttt aaagtcatct ggtttgcttc tcgccagttt
     4201 gaatgcctgc gcggtcagat ccgtaatggt ttctttccct ttgaaagtgc gtgtaacaaa
     4261 caaagctgtc ccaaatgcct cgtatttttc gcagattgct tcgttttttt catcgtcggc
     4321 attgatttca ttgagccgaa ctttttttat ttgggtttca gcactgaagg attctttcaa
     4381 gacagcattt acacaatccg tgatagcaat acacgaagcg cattttcggg ttgcatgaaa
     4441 aagcatgact gtcaccgaag gtgtttccgg cataaaggct ccgttgtttt ccggcccgga
     4501 gacttgcttg ccggaatctg agattacaga atctgaaaca acagaatcaa tggtttccgt
     4561 ctgaactgaa tctatatttc cagcagtttc ttgtttattg cctccgcacg aagccagcat
     4621 cagcagagca ataacagcga ataaaaaaat tattttcctt tgcatgtggt tggatttaaa
     4681 aagtgttcga ataatttttt tgctttttcc caattctttt tatttacaca ataacgtata
     4741 gtcgggagtt caatttcgcc ctgaatcaga cctgcatttc gaagttcttt cagatgttgc
     4801 gataatgttg agcgggcaat ggggacttcg gtaatcattt caccgctggt gcaacagccg
     4861 acgccgtcgc agcggttttc ctgaatcagc ctttccatta tatatacgcg cgcgggatga
     4921 ctcaatgcct tggcgtacat tgccaattct ttgtgctccg gtttaaattc taccttcttt
     4981 ttcattcaaa ctattttgcg tttgcaatga tttctttaat ctcacttact gaaggcacgc
     5041 ggcctgaaag gacgaccttt tcgtcaatca ccagacccgg ggtacgcaaa atgccgtaac
     5101 ccatgatttt catgatgtct tcctctttta caacattggc ttcaattccc aactcctcaa
     5161 ccgctttgcg tgcagctgcc tcaagatttt tgcaattggc acaaccagtg cccagaactt
     5221 tgacttccat aataattcat ttttatattt cgcaaaaata cgaaatgttt tttatatttc
     5281 gcaaattgac gaaatgtttt tttacccgga catcctgtcc gcccactggc gcaattccag
     5341 gtcagcaaga ctgtaacaat ccaacgcaga gagattgctt cgcaatgctc tcaggttgga
     5401 ttgacgcaaa atatcgggga aaattaccct tttctttctc ttgggcgcag ctggcacacg
     5461 caactgccgc tcaggcgctc gtttgtatcg accaccacgg gcataccgtc gcgctcccat
     5521 tctaccagac cgccggccag attgatgatg ttttcaaatc ccagatcctt caaaacaatc
     5581 attgcttcgt ggctgaataa accaaccgaa tcagccacaa tgagtacacg cttatcgggc
     5641 agatgcgagg caaattcctg taatttaata tggtgacacg aaataaattc gggtactcca
     5701 aactgtttga aagccgtgag tgtcggatcg cgtacatcga gcaacagcgc gccattcaga
     5761 catgcctctt atgcatcttt cggagtcaaa ttcaaaaatc cggctgatgt gaaagttttt
     5821 ttaaaaatat tgtccgggat agagttcatt ttttcagatt cacaaaaaca acctcaaata
     5881 tacaatgcat ttaatgtgat tacaaaaaag cagaggatca ttttgacatt ttcctcaaat
     5941 attataattt ttgaggattt aattccttat atattatagt ttttgaggat ttgaattgaa
     6001 caaatcaata ttaaaaccgc ttcagatcag cttttttgaa atcccactcc ggtgtatgaa
     6061 tgtcatttga acaatcgaga ctataccagg ggctgatttc gctatttgag ggattgatca
     6121 gaatctgaat ttcctgagcc ggcatgtagc tctgagcaag cagaaagcgc ttctcaccgg
     6181 ttgtttcgtt tacaatcata tcaaccacaa taaccgcatg accgggtgaa ccaccctgga
     6241 tcaatacatc gccaggccac atgtcgtccc aatttacagt gttcagttct ttattgagcg
     6301 aaagtgtccc tgcatacata aatactgttt ccatataatt ccagagatca tcatgtgtat
     6361 tcgaagccgc gctccgtgca acccagctgc aattatttcc atcaacggaa atccgtttcc
     6421 cgctcatcca ttttgaataa tcggcgcgaa aaccgctggt gaaattgaaa tgaatgtcgc
     6481 tgaatttttt ctgctgccac aaatattccg cacgcagccg gataactgca tcggcgcact
     6541 gatgcaggtt tttgaatcct atgggtaaat caacaacagc acaatacacg ttgtcgggtg
     6601 acttttcgcg accatcgaaa agcttgactt tttcgccgcc gggcttcaac ggaagcgacc
     6661 gcagataaaa gccaaaggtt gtggaatcag catctggtct cacaaatcca tcaggtgccc
     6721 caaagcgttc ctgaatggtc agaccttctt tgttgattac tgaattattt acctgcccgg
     6781 atgattggtt gccgcatgaa taaaccagca ccagtaatga aactgccgca aaaacttttc
     6841 ggaaatactt tctatttact ggcaacatcg ggatacaact ttttgaaatt gcattccagc
     6901 gggtttggat ttccgggttc gtgcagatgc atgccatccc cgcggcacca cttccaaagg
     6961 tcgcaatctt tacacatacc agttttaaac ggagtacggt cgcgatattc tttgaaacgg
     7021 ttgtcccata catcgaggaa atcgtctttg taaatatttc cctgcacaaa acccctatca
     7081 atattgggac acgcgccaat atcaccgttg gctaaaactg atccgacatg aattcctgct
     7141 cggcagaaga aaacgccgtc gcgcacgagg ccttcgtaat cgccgagaaa gccgtcgcag
     7201 ccggtcgcag ccttgattct gccttcggcg cgggtacgca aaataaaatc catgacataa
     7261 cggtactgat catttttgag catcaactct ggattcacag ccgcacggcc aatcggatct
     7321 atcataaaaa tacgccagcg cttcacgccc atttcaatca gaaaatccct gatttcatca
     7381 agctccgaaa ggttgcgttg attcacacag gttacaacat catacaccat ccctggctcg
     7441 gcgaccagca attttacagc gctcacggct ttttcaaacg acccttccct gccccgcagc
     7501 caatcgtgac tggctttcag tccgtcgaga ctgatggtgg cagaccttaa tccggcttcg
     7561 cgcagcgagc ggaaacgttc tggagtcatg gcgtaaccat tgctaaccat gccccaggga
     7621 aatcccattt ttttcagttc tgttccaacc atttcgagat ccttgcgtac aagcggctct
     7681 ccgcctgaga caacaatcat cactttattc ggattgtatc tttccgccac ctgacgacaa
     7741 acgttcagaa agtcttcggc cggcatgtca ggcgcgtttt caatgatttt gcaatcgctg
     7801 ccgcaatgca aacaagcaag gttgcagcgc gatgtacatt cccagaaaaa ataactcagg
     7861 tcgtgtaatt tggcttgcac acgcacatag cgcgcatgaa tattaaaaac cattcgtttt
     7921 ttcagcggaa attgtcttct cagtcttccc ataattcgtg tcagtttttt tctggtaatt
     7981 caagctcata agcaggcata gctccataac ctgtcggctg tatcggcgcc ggattataat
     8041 cgggtacaag cggtttttct actttctgaa cggcaatttt ttctacttca gtaatcattg
     8101 ccgagtcttg cctggacaat aatacgtcta tgtttttctc tttcttcaat gcagcctgcg
     8161 attgatcaga ttttgagtca cacgaaacca atacaaccat gccaagtcct gataaaataa
     8221 gactgcggca cagtatccat gcccagacct ttctgatatt atgcagtaat attttcatcg
     8281 tttttattaa gaacctggtc aaatattttt ttacatgtca aaatctgtcg gagggccgta
     8341 tttagtttga aatggcttgt catcatcaac cggaacaccg tatttagtga taggtttatt
     8401 tccgttcagg ttaggaacgc ccgtaacagt cgatgatgag tactgacctt gctgctcttt
     8461 tgctttttta atactgtctt cacgggcttt ggccaacgaa tcggcatgca cctgactcag
     8521 actatcttct ctcactttcg caatagagtc gagacgttgc tgctctttca ctttgtttaa
     8581 agaatccgga ttttgctgag aatctttttt atcacttccg gagcaggccg acagaaaacc
     8641 ggccagacca ataatgacaa gcatgttccg caacaggcgg gcattgagta acaacaaacg
     8701 gtaacggatt ttgtggctca tgtgatttgg ttttggatag tcaaagatag caaaatgctg
     8761 gtgtgcgaag taaaaatact tcacacgact ttttattgtt ttggaacttc cggaactttc
     8821 actccataga gcggaaccgg tcgggctgta tcgtatttgg gcgtaatctg aatcacaccg
     8881 tagtcggtct ggatttcggg attgtaataa ttgggctttg ttgtatcggc cggtgcaaca
     8941 atggctgaat ccggcatttg tggatctggc tctgagagct gcttttcagc agatttgcaa
     9001 ctgaccgcaa cgaccggcaa gctcaataaa atcagcaatc gctttaacag tgaagcattc
     9061 agttgatata aaaaaaattt actgtttatg ttcatacatt aattgcgcgg tacgccgtat
     9121 ttggtctgaa tctgctctgg tgatatttca aattcataaa ccggtggcac accatattcg
     9181 cagattggcc ctggatcatc aggaattaca ggagctacat aaacgcttac ctgttttttt
     9241 gcatcagcaa tactatcctg cttttctttt tcaattatag aatccaatct taatttgagt
     9301 gtttcgtttt catttaaaac ggagtcaagc tgttgattca attcaaccag ttttaaagaa
     9361 tctttgttaa tagcagtctg tttttcagaa acaccattac acgaagaaat aagggcagaa
     9421 aagctgagca ttgcgagcat gtttctcagg attctggctc tgagacggta aatccgataa
     9481 gcaatccgaa ggttcatgac agtgattttt ggaatttagt gcaagataag atgaattttg
     9541 gagaaaagtt cataaacaac agtgaacggt gagcggcgag cagtaagcta aatacgaaat
     9601 cagctttcag taatcagtat tcagtcgtca gccgtcgcct tatgcctgac gnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaatcagct ttcagtaatc agtattcagt
     9781 cgtcagccgt cgccttatgc ctgacgccta tcgactaacg ccttattgca tacaccacct
     9841 catcctcgag ctcgccgttt ttcacgtagg ttttttcgaa gcgggcttcg agggtgaaac
     9901 cggctttttc gagtacgcgc tgcgagccgg tgttgcggcc gaacgtgccg gcataaatgc
     9961 ggttgatgtc gaatgttttg aaaccatatt ccaccacttg ttttacggct tcggtcataa
    10021 tgcccttgcc ccagaacggc tctgcgagcc agtatccgat ttcagcgttg ttgcgcctga
    10081 tgtccgacag cgggaacagg ccaatcgagc cgacagcttc atcgttgatt tcaatcgcaa
    10141 atattcgtac gggatctgcg ctgcgggcca ttttcagaaa ccgctttccc gattgttttg
    10201 tgtaggggtg cggaaacatg tcggttagat tcattgctat gcgcgggttg tcggcatatt
    10261 tgagcagata tttcagatcc gattttctcc agggacgaag cgtgaagtcc atagcggcag
    10321 tttttacgac attcaaagat agcggaattt tgccacgggg gcacaaagac acgaaatata
    10381 tcttcctcta agaacgcaaa tgagcgctaa attactgctc tgcctcagca tggcttattg
    10441 acgcgaattc gaatcagcct tcatctgcga tgaagacagt atcggggcgg cgcacggaga
    10501 tcgctgagac gcagagagtt tcgatagtta tcggaattgc cacgagggca taaagacacg
    10561 aaacaattaa ccactaaaaa cccggtcaat gttttgacaa ggcttctgca accaaaccgg
    10621 gatctccccg aaaaccgact cagcatatcc actttttata cacgaattaa aatcatccgc
    10681 gtaaacctga ctgcaatttc aactgcaaca ggcagtcagg cagatccgcg tccaatcaac
    10741 gctacgccct accaaactgc atcaggtacg ccttgataaa gtcgttgatg tcgccgtcga
    10801 ggacggcctg ggtgttggat gtttcgtgat tcgtgcggtt gtctttcacc atcttgtagg
    10861 gatgcagcac atagctgcgg atttgtgaac cccactcaat tttcttttta ctgctttcga
    10921 tggcgtcaat ggcttcgcgt tttttgcgca actccagttc gtacaactgc gatttcagca
    10981 tctgaatggc cttctcgcgg ttctgaagct gagagcgcgt tacctgacat tcgaccacaa
    11041 tgcccgaagg cgcatggcgc acacgcactg cggtttcaac cttattcacg ttctgtccgc
    11101 cggggcccga gctgcgaaag gtatcccagt cgagatcggc agggttaaga acaatatcaa
    11161 tattttcgtc cacaacagga tatgcataca ccgaagcaaa cgaagtgtga cggcgctcat
    11221 tggcatcgta gggcgaaagc cgcaccagac ggtgcacacc gctctcgctc ttgagatagc
    11281 cgaatgcata gtcgccttcg atttcgagcg agcatgattt gattccggcc acatcgccct
    11341 cctgcaggtc gagttcagta actttgtaat tattccgttc ggcccaacgc acatacatgc
    11401 gcatgagcat ttcagcccag tcctggctct cggtgccgcc tgcgcccgag ttgatggtga
    11461 tgatggcact gagtttatct tcttcgccgc tgagcatatt gaaaaactca aggtcttcaa
    11521 tcgctgtctc agccacggaa tacgtctgat caaactcttc ctgggtcata tcgccgcctg
    11581 cgaaaaattc cagggctaca tcaagctcgt caacagcaag tgaaaccttg tcgtatcggg
    11641 taatccagga ttttttttca ctcagggatt tcagaaactt ttcagcttct ttgggattgt
    11701 cccagaaatc gggggccaat gatttttttt cttcttctct gaaatcggat cttctgcgat
    11761 cgatgtcaaa ggaacctcct cagagcgtcg aggcgctcct tcaatgtttt tacgtcgtct
    11821 tttcctgcca tggtggtaga attttgtgca aagataagaa atagtggcca gtggtgagtg
    11881 gtcagttttg agtgaaaatc aaactgtctt ctggcgagtg gcgagtgatg agttatgaat
    11941 tatgagttat aagctgatat acatttataa tttgttgtgt aaaagaaaat cgcgtgatgg
    12001 ccattggtga aaaagtagta tttttgattt gagttcaaga atcaatggat aatttcgaag
    12061 aatatttgcg acagggtgag ccggacaaag ctgaaaaagc aaaagtctgg aaaacggctg
    12121 tcggcttgca ggatgttgac ggactaaagc cgtctgacta tctcattgaa actgcccggc
    12181 aaaatatcga gggcgacatt actattgatg aagtcaagca gcgcattgac acttactata
    12241 agcagcatcc acaaaaaaca gacgaaagcc gtaccgagga agcagataaa gtttcggccc
    12301 ggattgcgga aattctgagt gaaaaaacat ttacattttc gccggcagaa tacctaagca
    12361 ttcatcgcag gttatttact gatgtttata aatttgcagg caaaatacgt gattacaaca
    12421 tcaccaaaca ggagtgggtg ctggatggag aaactgtttt atacggaagt gcggaaagct
    12481 tgaaagccac acttgaatac gattttgaac aggagaaaaa attcagttat aaaggattaa
    12541 gtcagcatga agttattgag cacattgctc gttttatttc ctatctctgg caaattcatg
    12601 tatttggaga aggtaacaca cgtgcaaccg ccgtttttct gataaaatat ttacgcactt
    12661 ttggcttcgt ggtcaataat gatttgtttg cagagcattc atggtatttt cgaaatgcac
    12721 tggtaagaag catttataaa aaccattcaa ggaaagttta cgcaaccaat gaatttctgc
    12781 ttcgttttct tgaaaacctg ctgctgggcg aaaatcatgt tttaagaaac cgtgaaatgc
    12841 acattcagct tgccgaccct gcaaatgacc ctgtaaaatc aaaaaatgac cttgtaaatg
    12901 accctgtaaa aagcagtatt ttaaatcatc tgaaacaaaa tcccaaagcc aattacattg
    12961 aacttgctga aaatattggg tgttcaacag ccaccattaa acggcacatt caggagctta
    13021 aaaagaaggg actcatagaa cgcatcggca gcgacaaaac ggggtattgg cgattggtgg
    13081 aatgacaaca gctattatcg taaaatcaaa atagtattgc tgaggaataa aaaagggaca
    13141 aaattgtgtc cctttttcaa tatcagcttt tcggttttac ttcaagccat cgtgtggctt
    13201 ttacaacaaa gcgaacaaag aggtatatca gcaagcccgc tcccagtcca atcatccatt
    13261 caatatcgat cctcgattct ttggtgtaga aaagatgttt gagaaaatta atgatggcaa
    13321 atgacagaat ggtggcgaaa aatccgtgat attcgcgtcg catcacactg cgccagttga
    13381 atctgcgacc ggtttttaca taaccgctgc atttcggaaa aaacgccggt gttttagcgg
    13441 cccagtcttc gaatgcctga ccgaattttc cgccaataaa gcgctcttcg gcaaacatga
    13501 tacgttcgta gtaaatccag aaaaagaata ccgcaaaaat gagaaattcc cagctgccca
    13561 cataaatgat cagcccgagc cacatgagaa aattgcccaa gtacagcgga tggcgtacca
    13621 ccgagtataa accggtcgta ttgatggtgt cggcaatctg ctttccggtg ttgcggcccg
    13681 atgttccgcg cggcgcataa ccaatggcaa tggcacggat tatcattcag atggcggaaa
    13741 caccaaggca gataagactc caccagtatt cctgatacgg gataaattca tccttgtcga
    13801 gccagatggc caaggcagca aaaacatata gaacaagcgg tataaaactt ctgtatttga
    13861 aaaggcggtt gccgctcttc tcaaattccc tgatcattga cataggcggg acttatttca
    13921 gtaaggatag aaagcgaaat cggttccgag atagtatgac tggccaccca gcatatcttc
    13981 gatgcgcatg agctgattgt attttgcaat acgctcgctg cggcatgcgc tgccggtttt
    14041 aatcaggcct gtattcatgg caacagccag atcggcaatg gttgtatctt cggtttcacc
    14101 gctgcggtgg ctgatgatgg aagtgtagcc atatcgatgc gccatgttga ttgcctgaat
    14161 ggtttcggaa acggttccga tctgattcag ctttatcaga atagagttgg caacaccttt
    14221 ttcgatgcca tcggccaaac ggttaacgtt ggtcacaaac aagtcatcac caacaagctg
    14281 aacattcgca ccaattttat cagtaataat tttccagcca tcccaatcgt cttcggccat
    14341 gccgtcttcg atggagtaaa tcggatattt ttttgtccaa tctgcaagga acgaagccat
    14401 ttctttggag ctgagcttat caccggtcga tttcttgaaa tgatattttt tatcggtagt
    14461 gttgtaaaat tcgctggcag ccacatcaag tgcgatggct acatcaacgc ccggtttata
    14521 tttggctttt tcaatggctt caatgatgac ctgcaatgct tcttcgttcg atttcaggtt
    14581 gggtgcaaag ccaccttcat cgccaacatt ggtagagaga ccacgttttt tcaaaacatc
    14641 tttcaatgaa tggaaaactt cggtacccat gcggatggcc tgattgaagg tttctgcatt
    14701 cagcggcatg atcataaact cttgaaaatc gatgctattg tcggcatgcg agccaccgtt
    14761 gatgatgttc atcataggaa tcggaagtgt gttggcattt acaccgccga gataacgata
    14821 gagcaattgg tttgtttcag cagcagcagc atgagccaca gcaagcgaca caccaaggat
    14881 tgcattagct ccaagacttg atttgttttc cgttccgtca agctgaatca tcaggcgatc
    14941 aatctggttc tgatcagtga catcgagacc ctgaatttcg ggagcaatga ctttattaac
    15001 attattaacg gctttgagca cgctcttacc gttgtacatt tttttatcgc cgtcgcgaag
    15061 ttcatgagct tcgtgaattc cggtagaagc gccagaaggc accgaagcac gggcaaagtt
    15121 gccatttgag gtaactaatt caacttctac cgtaggattt ccacgggaat cgagaatctg
    15181 tcgggcgtaa acatttgcaa ttgttgccat aatattttgg tttaaaaatg aaagacagga
    15241 tatccgaaaa tatcctgtcc aaaagtatga aaaaattcag tacgtcaaat tatttctcag
    15301 caccgtcagt ttccggagcc tgctcatcgg caggtttttc ttcgggaaca tcaacagctt
    15361 tcacatcttc cgctttaggc agttttgttt cagcggcagg agtttcgtct gatttttttg
    15421 ctttcgagcg acgagtacgt ccagtagttg ttttcttggc ttctttggta ccgagcatca
    15481 cttcgttgta atccaccagt tccatcatac acatttcggc attatcgccg gcacgcatat
    15541 ctgttttcag aattctggtg tacccaccgg gacgatctgc aattttatta gaaacttcgc
    15601 gaaagagttc tttcacagct tctttatcct gcaggtattt gaaagccata cggcgatggt
    15661 gcattgagtc ttctttggaa agagtaataa ccggttctac aaaacttcgc aatgctttgg
    15721 ctttttgcag agtagtggtt attcgtttat gcaggatcag cgaagatgcc atgtttgaca
    15781 acatagcttc gcggtgagcc gtttttcttc caagcttatt aaatccgttt ttgtgtctca
    15841 tctcggttaa tccttatcca gtttatattt tgatacattc attccgaagt gcaggttttt
    15901 cgatttcact agttcttcaa gttcggccag tgatttcttt ccaaaattcc tgaatttcag
    15961 aaggtcattt ttattgtagg agacaagttc gcccagtgtt tcgacatcag cggccttgag
    16021 gcagttgaga gcacgaacac tgagatccat atccgacaat ttggttttga gcagatggcg
    16081 catgtggaga taatcttcgt caaaatcatc aacaacggct ttctctgttg gttcgatatc
    16141 aattttatcg tcggagaaga gcgcaaaatg ctgaatgaga atagcagaag catctttaag
    16201 agcatctttg gggagaattg atccatcggt ttgaatcgtc agaacgagtt tttcgtagtc
    16261 tgttttctgt tcaacgcggt agttctcgat gttgtaattt acattggtaa ttggtgtgaa
    16321 tgtagcatcc atagcaatat aaccaatgtt ttcggttgca ggtttaaggt cttcggcagc
    16381 aacataacca cggcctttct ctatattcag ctcaaagctc aacttcacag tcgggtcgag
    16441 gtgacagatc acgagatccg gattcagcac ctgaaatccc gagaggaatt tgttaaggtc
    16501 tccggcagtg actacttcct ggccttgaaa aacagcgttt acatgttcag aattgagttc
    16561 ctcaatctgg cgtttcaggc ggacctgctt gagattaaga acgatatcca ccacgtcttc
    16621 cacaacaccc tgaatggtgg agaattcatg aaggacattg tcaaatttca caccggtaat
    16681 gctaaagcct tcgagcgaag agagcaaaat tcgacgaagg gcattgccaa tggtaatgcc
    16741 gtagcccggt tcgagaggtc tgaattcaaa cactcctttg cggtcgtcgg attcgagctg
    16801 aatgacttta tccggtttct gaaaattaag tatggccata gtttgaattt atggttgtta
    16861 tttgttgtac aattcaacga tcaactgttc attgatggct tcaggaatgg cttcacgttc
    16921 cggataattc aggaatttac cagccatttt ttcgccgtcc cattcgagcc agctgaaatt
    16981 attattgtgt ccactcaatg attcagtaat gatctcgagg tttttcgatt tttcacgaac
    17041 tgaaaccaca tccccgacgc gaagaaggaa agaaggcaca ttgaccactt tgccattcac
    17101 ttcgatgtgc ttgtggctaa caagctgacg tgctccatcg cgggtcttgg caataccaag
    17161 gcgatacacg gtattatcga gacgtgactc gataaactgc aggagttttt caccagtaac
    17221 accttttttg gtagaagcca acagaaagag ttttttaaac tggcgctcaa gaatgccata
    17281 agtgtatttg gctttctgtt tttctgcgag ctgaacacca tattccgaaa cttttcttct
    17341 gcgtttcgac tgaccgtgca ttcccggtgg atactttttc ttttctaaaa atttgtcgtg
    17401 accgtaaata ggttcctgga acttacgggc aatttttgac tttggtccga tatatcttgc
    17461 catatcttgc tattttcaac agttaattaa acccttctgc gtttgggagg acggcagccg
    17521 ttgtgaggaa taggagtaac atcaacgatt tcggttactt cgataccagc accatgaatg
    17581 ctgcggatgg cactttcgcg tcctgatccc ggaccgcaaa catacacctt cactttacgc
    17641 aagccagcat cgaaagcgac ttttgcgcaa tcttcggctg aaacctgtgc agcataaggc
    17701 gtgtttttct tgcttcccct gaagcccatt tttccggctg acgaccagga aataacctgc
    17761 ccgtcttcgt tggtcaacga aataatgata ttgttgaagg atgccttcac atatgcattt
    17821 ccgaaaggtt caaccttaac cttacgtttt ctgaccgttt tagtttgttt tgccatatct
    17881 ggatattttc aaattttatc aacccttagg tgccttcttc ttgttggcaa ctgtcttttt
    17941 ctttcctttg cgagtacggg cattgttctt ggtactctgt ccgcgcaacg gaagaccgag
    18001 acgatgacgg atgccacgat agcaacctat gtccatcaag cgtttgatgt tcatctgtac
    18061 ttcactacgc aaagcgcctt cagtcttaat ttcatcactg atgactgtac ggatagcggt
    18121 cagatcgtta tcatcccaat cctgtacttt tttgtcaaaa tccactttgg cttttgtcaa
    18181 aatcatgcgg gaagtcgaac ggcctatgcc ataaatgtag gtcagcccta tttcgccccg
    18241 tttgtttttt ggtaaatcaa ttcctgcaat acgtgccata tattgttttt ttcaaattcg
    18301 ttatcagccc tggcgttgtt taaacttagg gttctttttg ttgatgatgt acacacggcc
    18361 tttacggcgt acgattttac aatccggtgt gcgtttcttg actgaagctc tcactttcat
    18421 agcgctgtcc tgttttttat ttgtatctga aggtaattcg tcctttggtc aaatcatagg
    18481 gtgaaatttc gagtcgaacc ctgtcgccgg gcagaatctt gatataatgc atgcgcatct
    18541 tgccggaaat gtgggccaga ataacatgcc cgttttccag ttcaacgcgg aacattgcat
    18601 tgcccaagct ttcgacaacg gttccgtctt gttctattgc accttgtttt gccatatttt
    18661 gttttacact ctgttttcta tgtactcata tgttgaaatc acttccggag tgtccctcac
    18721 caccaccatg tgctcgaaat gcgcactatt gctcccatct cgtgtgcgga ttgtccatcc
    18781 gtctgcctcc tgataaacgc gccaggttcc cagattaatc attggttcaa tacagattac
    18841 catattttca accagtggca taccatgacc acgtctgcca taattcggca cttctggttt
    18901 ttcatgtaaa tgctgaccaa ttccatggcc aacaagatcg cgaactacat gaaatccatt
    18961 acgctcaaca tgaagctgaa cagcatggcc aatatctccc acacattttc ccggcactgc
    19021 ctcggctatt cctttcagca atgattcatg cgtaacacgc accagatcca ttgttttccg
    19081 atcggtttca ccaacgataa aggtgtaggc ataatcgccc acatatccgt tcagaactgc
    19141 accacaatca actgaaacaa catctccatc ctttatcacc cggcttccgg gaattccatg
    19201 aaccacctga tcattaatag aaatgcacag tgtggcagga aatccattat agcccttgaa
    19261 ggcagggaca ccgccattat cgcggataaa ttcctctgca gccctgtcaa gggttcgtgt
    19321 ttccactccc ggtttcagca ggcgggatac tactcccaaa gtttttccaa caagcaaaga
    19381 actctttctc agtaattcta tttcttcatc acttttctgt aacatggcta tcagatacct
    19441 cccattccgg tcgaagtacg gcctttaata cgacccgact tggtcaaacc gtcataatgg
    19501 cgcatcagta aatgactttc gacctgttgc agtgtatcga gcactacacc aacgagaatc
    19561 aacaaagaag taccaccata gaactgggca aactgactat tcacaccaaa caagctaaca
    19621 atagctggca taattgcaac caaagcaagg aacatcgaac ccggcaaagt aatacgcgac
    19681 ataacggtat caataaattc cgctgtgcgt tttccaggtt tgatgcccgg aataaaaccg
    19741 ccgtttttct tcaggtcctc agccatctga ttgggattga tggtgatagc agtgtagaaa
    19801 taagtgaaaa gtataataag aattgcaaag acaaaattgt accagaatcc gtttggattg
    19861 gtaaatgccg caataacacc agtgagagat tcactgtttg caaaaccggc aagagtaata
    19921 ggaataaaca taatcgcctg agcgaaaatg ataggcatta caccggcagc attaaccttt
    19981 aacggaacat attgccggac cccaccatac tgtttgtttc ctaccacacg ttttgcaaat
    20041 tgcactggaa tttttcttgt tccctgcacc agcaaaatag tcataacaac aacaagaaca
    20101 agaataacca gttcaacgat aaacaccaca agaccgccac cagcaacttc catacgagaa
    20161 gcaaactcgg caagcaaagc aaatggcaga cgagcaatga taccaatcat aatgattaat
    20221 gaaataccat tacccaaacc tttatcagta atacgctcac caagccacat aacaaacaat
    20281 gttccggcgg tgagaataat ggttgaagac agtcggaaaa ggaaaggcac ctgcgaaatg
    20341 gcagcatcga aaggataaat accaccggca ggaatctgcg aagcgagatt agcaatataa
    20401 ccctgtgcct gagcagcagt aataataact gtcaggtaac gggtaatctg attcattttc
    20461 tttcttccgc tttcaccttc acgctggagg cgctgaaaat aaggaacagc aataccaagc
    20521 agctgaacaa cgatagaagc agaaatataa ggcataattc caagagcaaa cacagaagca
    20581 ttggaaaaag caccaccgga aaacatattg ataagtccga gcagaccgtc ctggccctga
    20641 ttctgcaaag catccagaga attgggatca ataccaggca aaaccacgta ggatccgaaa
    20701 cgataaatca aaatgatcag caacgtgtag aggattcttt ttttaagatc ctcaattctg
    20761 ttgatattct tgaaggtatt gacgagacga ctcattaaac ttagattttg attgcttgac
    20821 caccttgttc ttcaattgcc ttaacggcag cagttgagaa agcatgtgct ttcacatcga
    20881 gtttaagggt gagttttcct ctgccgagga ctttcacgat atcatttttc gaaatcagac
    20941 catgagcgcg aagcacttcg ggggtgattt cctgaaggtt attttgctga gcaagttcct
    21001 gcagcatgtc gatattgagc gcgcggtatt caacgcgatt gatatttttg aaaccgaatt
    21061 tcggaacacg acgctgcaga ggcatctgac cgccttcgaa tccaatctta cggctgtaac
    21121 cactacgtga ctgggcacct ttatgaccac gggttgatgt tccaccgtga cctgatccct
    21181 ggcctctagc gatgcgtttt tctctttttg ttgctccggc tgccggtttt aaattactta
    21241 aatccattgt tgtcagtttt ttaaggtcag atttcttcaa ctttcaccag gtgattcact
    21301 ttcctgatca tacccaaaat ttgtggggta gccacgtgct caacgcttga atgcattttc
    21361 ccaagaccca atgcctgaac atttttgcgc tgaatggccg gacggtcaat cagactttga
    21421 attaatgtaa tgcgaacttt tttcatattt ctccggatta accgttaaac actttatcaa
    21481 ttgaaacgcc acgaagctgg gcaatcatgc ggggatcttt cagattcatc aacgcatcta
    21541 cagtagcttt tactacatta tgaggattgg atgaaccttt tgatttggcc aacacatttt
    21601 ttacgccaac actttcgagc acggcgcgca tagcaccacc agcgatgact ccggtaccag
    21661 gcgctgctgg tttaaggaat acggtagctc cgccaaattt tccctcctgc tggtgaggca
    21721 aagttccttt caggatgggc acacggacaa gattcttttt ggcatcttca attccttttg
    21781 agatggctga ggttacttct ttggcttttc caaggccata tcctacaaca ccgtcttcgt
    21841 ttccaacaac cacaatggct gagaaactaa aggtgcgacc gcccttggtc actttggtaa
    21901 ccctttgaat gcttacgaga cgatccttga attcaatgtc gctcgatttt actctgttta
    21961 tactgatatt agccatatta ctcagaattt taatccacct tctctggctg catcggccaa
    22021 agcttttact ctaccgtgat actgataacc gttacgatcg aaaaccactg actgaacgcc
    22081 agcttcgatt gcacgttctg caaccagttt gccaaccagt ttcgccttat cggtttttgt
    22141 aactttctgt tcggcaacgc ctttcacgcg tgatgatgcg gatgccaatg ttttgccaga
    22201 tttgtcatcg ataatctggg cataaatttc cttgcagctg cggtacactg acaaacgcgg
    22261 acattcgccg gttcccgaaa ccactttgcg gatacgcatc ttgatgcgtt ttctgctgaa
    22321 ctcttttctg ctcttgatag ccatagttgc ctcttttatt atttagccgc agcagcttta
    22381 ccagctttac gacgtaaaac ttcattttga aatctaacac cttttccttt gtacggctca
    22441 ggtttgcgga acgaacggat tttggctgca acctgaccaa ttaactgctt gtcgtgcgac
    22501 ttgagtatga tagtcgggtt tttacctttt tccgttatgg tttcaatctg aacttcctta
    22561 ggtaattcga acaaaatatt gtgcgaatga cccaaactaa gatccagcat ttgaccattg
    22621 gcctggcatt tgtaaccaac accgacaagc tccatggttg tggtgaatcc ttcagagacc
    22681 cctttaacca tgttggccag caatgaacga tacaagccat ggaacgatct cacctgacgt
    22741 tcatcattag aacgattaag atgaagaaca gaaccttcaa tgttaatttt cagttccgga
    22801 gccacctgct gtttcaattc gcctttggga ccttttacgg taacgatatt atcggcagcc
    22861 acgttcactt gaacgcctga gggtatatca atctgttttt ttccgatcct tgacatttgg
    22921 aatcctcctg ttattaatta tgacacaaaa caaatgactt cgccgccaac gttttctttg
    22981 cgggcctgtt tgtcagtcat gagccccttc gaagtactga taatggcaat gccaagaccg
    23041 ttcatgactt taggaagttc atcacttgga gagtacttac gcaaacctgg acggctcacg
    23101 cgttttatct cggtaatggc cgacagttta ttaatggggt tgtacttgag ggccacttta
    23161 agggtccgcg gcatagtact ttcctcaact ttataattga gaatatagcc gttctcgtgt
    23221 aacaacttgg ccagttcaac cagcattttg ttggaaggaa cctccacaat acggtggttg
    23281 gctttgatgg cgtttcttac gcgggtcaga aaatctgcaa tggaatccgt taccattcta
    23341 tattgtttta tattttacca acttgctttt tttactccgg gaatttctcc gttcagggcc
    23401 atttgcctga aattgatgcg cgaaattcca aacatgcgca tatatccttt tggacgtccg
    23461 gtcagtttgc aacgattatg caatctgatt ttcgaagcgt tcttgggaat tctctgcaga
    23521 gcagtgaaat caccggcggc tttcaaagct tctttctttg cagcgtattt ttcaaccatt
    23581 tgcaaccgct tcacttcgcg ggctttcatt gattcttttg ccatacctta atgtttttta
    23641 aagggtattc cgaatgattt taacaatgca tgagcttctt tatcgttagc agctgaagtc
    23701 acaaaagtga tatccattcc ggtaatcttg ttgattttat caatgttgat ttccgggaaa
    23761 atgatctgtt cagttactcc gaatgtgaaa ttaccacggc catcgaatcc tttgtcactg
    23821 attcctctga agtcgcgaac gcggggaata gaaacagaaa tcaaacgatc gaggaattcg
    23881 tacatgttgt ttccacggag ggtaacacga acaccaaccg gcattccctt gcgcaccttg
    23941 aagtttgaaa tatcttttct cgatttggtt tgaacagctt tctgaccggt aatattggtc
    24001 atttcgtcca ctgcaaaatc aatgagtttt ttgtcggtaa cagcaccacc aacaccctgg
    24061 ttaacagata ttttgaggag cttaggcacc tgcataactg ttttgaactg gaattcctgc
    24121 atgagagcgg gaacaatttc cttgtcgtat ttttctctta atcgcggaat gtaagccatt
    24181 acttaatttc ctcctttgat tttttggaat atcttacgat tgtaccatct tctttcacct
    24241 gacgacccac gcgggtaggt tttcctgatt tatcaaccag catcagatta gagatattga
    24301 tgggagcttc ctttttaaca ataccacctt ttgagtcttt ggcggaaggg cggctatgct
    24361 tggaaacgat tttcacgcct tcaacaatgg cagtgtttga atccggaaaa acttccagta
    24421 cacgtccttc agttccgcga tcgtttccgg aaatcacctt cacagtgtcg ccttttttaa
    24481 ttttaatttt cactttcata acgctgcgaa ttataatacc tcaggggcaa gtgaaacaat
    24541 tttcatatac tgtttgtcgc gaagttcgcg cggcactggg ccgaaaatac gtgttccacg
    24601 caattcatcc gaagcggtaa gcagtacaac tgcattatca tcaaagcgga tatatgaacc
    24661 gtcgggacgg cgtatttctt ttttggtgcg aacaacaact gctttagaga cagtaccttt
    24721 tttgatattt ccggaaggga tggcatcttt caccgaaacg atgattttgt ctccaaccga
    24781 agcataccga cgtttggtac cgcccaggac acggatgcaa agaacttctt tggctccgct
    24841 gttgtcagct acatttagtc ttgattcctg ctgtatcatg gttatttagc cttttcaatt
    24901 atttcaacaa gtctccagcg ttttgtttta ctcaacggac gggtttccat tattcggacc
    24961 ttgtctccga ttgtgcattc gttcttctca tcatgagcca caaatttggt agttttttta
    25021 atgaacttac catacttggg atgcatgagg cgacgttcca ccagaacaac tgcagactta
    25081 tccatcttgt tgctggttac tacaccgatt cgctctttac gtaagtttct ttccattatg
    25141 ctgaaaaatt acttgatttc caattcacgt ttacgcagtt ccgtttggat acgtgcgatg
    25201 gttttctttg tttgtccgat cacgccagga ttatccagcg gatttactgc atggttcatc
    25261 tgaagtttgg caacctgctt tttctcttcc gtcaggcgct ctttcaactc ttcagtggtc
    25321 atttctttaa ttacttctga tttcattgct ttttcctttt ttatgaacta tgcttcgctg
    25381 taatcgcggc gaacaataaa tttggttttc acaggcaatt tctgagctgc aagacgcaat
    25441 gcttcttttg caacttcaag tgaaacgcct tcggcctcaa acatgatacg accaggagta
    25501 ataggggcaa caaaatattc cggagcacct ttacctttac ccatacgtac ttcagctggc
    25561 tttttggtca ccggtttgtc aggaaatatg cgaatccata tctgtccttc acgtttcata
    25621 taacgggtaa gagcctgacg ggcagcttcg atctgacgtc ctgtaatcca tgcttcttcc
    25681 atggttttca atccgaatga accaaatgca agctgatgac cacgtttggc attgcctttc
    25741 attttgtcct tctggactct tctgaattta gttttctttg gctgtaacat gactcttccg
    25801 tattattgat tagagttatt ctttttacct cttggcttgt tgaatccacc agggcgctga
    25861 ccaccgcggt cacttccacg atcaggtctg tctccgcgac gttgtttttt gtcggttttc
    25921 ggtgtatcaa ttccgtagaa atccggtttt ccaaacattt cacctttgca gatccacact
    25981 ttgatgccaa gacgaccata agtagtatga gcttcggagc gagcgtagtc aatatcggcg
    26041 cgcagagtat gcaacggagt acgaccttcc ttataatgtt cagtacggct catttcagct
    26101 ccaccgatac gaccggaaat agtcactttc atgccttcgg cacccatgcg catcgttgat
    26161 gcaacagcgg ttttaatggc tttacgatgc gacatacggc cttcgatctg acgggcaata
    26221 ttattggcga ccagaacagc ttccacttcg gggcgtttga tttctgaaat attgatctga
    26281 acttctttct gagtaatttt tttcaattct tcgcgaagtt cgtcaacatc cttgcctttc
    26341 tgaccgatga tgaaaccagg tttagcagta tgaatggtaa cagtcacaag cttaacactg
    26401 cgctcaatga atatttttga aacattggct ttagccagac gtacgttgag atacttacgc
    26461 accttgtcat cttcgacaag tttttcaccg aaagtttttc caccgtacca gatagaatcc
    26521 catccgcggg tgataccgag tctgttgcct ataggattag ttttttgtcc cattctgctc
    26581 tttattcttg tgtttcaaca ttagttactt cttccgttct gctacccagt tcaatggtaa
    26641 catggttcga acgttttttg atgcggtgag cccgtccttg cggagcaggc tgaatgcgtt
    26701 tgagcatacg gccaccatcg acattgatgc ttttgacata caaaccggct tcttccattc
    26761 tgattttatt ctcagttttc tgttgccagt tattgatggc tgatgtaagc agtttatata
    26821 aataatctga cgacgaattt gacgatgtct tcagaatagc cagtgcgata tcggcgtttt
    26881 tacccctgac cagattggcc accgggcgca ttttgcgcgg agtaatgggg caacccgtca
    26941 acttggcagt aaactgctct ttgcgggctt ccttacgctt gtcggcactt atccgttttc
    27001 ttgctcccat gacaattacc ttttaatgat tatttcttgt tttgaccggc atgaccacgg
    27061 aaaatgcggg ttggagaaaa ctctccaagt ttgtgaccca ccatgttttc ggtaacatac
    27121 acgggaatga atttgtttcc gttgtgaact ccaatggttg cgcctacgaa atcaggggtg
    27181 attactgatg cgcgtgacca agtgttgatg gtcacctttt tactggattt ctgtgcttcg
    27241 ataactcgtt tttcgagttt gtggtgcacg taaggacctt ttttcaatga gcgactcata
    27301 gtgtatccct gttatttttt acgacgttca atgattaaat tatcagaggc cttttttctg
    27361 cgagtcttgt atcccttggc aggaatacct ttgcgcgaac gaggatgacc tccggattga
    27421 cggccttcgc caccacccat tgggtgatcg actgggttca tagccacacc acggttacgt
    27481 gggcgacgac cctgccaacg gctgcgacca gcttttccac tgacctcaag attatgctca
    27541 gcatttgaaa cagtaccaac ggtagcacgg catgattgca aaatcaaacg tgtttcacca
    27601 ctgggcattt tcacaactgc atatttgccg tcacgagaaa gcaactgcgc ataagaaccg
    27661 gcactgcggg caagttttcc accctgtccc ggattcagtt caatgttgtg aataactgta
    27721 ccaaaaggaa tttcacttaa gtacattgta tttccgattt caggtgcaac acctttaccg
    27781 gaaaaaattt cctgtccgac tttcaaaccc tgaggtgcaa tgatataacg cttctcgccg
    27841 tctttgtaga acagcaaagc aatacgggca ctacgattag gatcgtattc aatggtttta
    27901 acggttgccg ggatgccttc tttttccctt ttaaaatcga ttaaacgata cattttttta
    27961 tggccaccgc caatgtagcg catcgttctc tttccggtgt tgtttctacc accagatttt
    28021 cgattgggaa ctaccaggct tttttccgga gccgctgccg ttatttcctc gaacgaactc
    28081 accagtttaa aacgttgacc gggagttgtc ggtttaaatt tcttcagtcc catgcagtct
    28141 attttgtttt atacgttact gtaaaagtca attttctctc cttcttttaa agttacaata
    28201 gctttcttga aagcattggt gcgaccctga acataaccac gacgtgttcc gcggaattta
    28261 cgtttgccgg catacaccat tgtattaacc tcaagaactt taacactata caaagattca
    28321 acagcttctt tgatctgaat cttgttggca cgctttgcaa cgataaaacc aaactgattc
    28381 agctcagtgt tcttggctgt catcttttct gttaccaatg gtcttattaa tacgtccatt
    28441 ttttcaattt tttagtgtca ttcctgacgt cattattctc cttctccaag aaaaattgta
    28501 tccagttggg gcaaacttgc ttcggaaatc aacacgatct gagcattcag aatatcgtaa
    28561 gtgtttaatt ttgaagcatt tatcaccttg accttcttca aatttcggga cgacaaatat
    28621 acgtttttct ttgattcatc caacacaatc aaagattttt tttctgaagt ttcgaaattt
    28681 ttcagaattt caagcatctg acttgtctta ggtgcctcaa aatcaaagtt ttcgataacg
    28741 cggatggcac cttcatttac tttgtaagat aatgcacttt ttctggccag ttgctgaact
    28801 ttcttgttta ccttaaaatt gtaatcacgc gggtgcggac caaaaacacg tgctccacca
    28861 tgaagcagcg gggatttgat gtcaccacga cgggcaccgc ccgtaccttt ctgacggaag
    28921 agcttcctgg tgctgccact aagcgtgcta cgctctgtac tgtcagcagt accctgccgt
    28981 ttgtcggcct ggattctttt aacatcaagg taaattgcat gatcgtttgg ttggatcgca
    29041 aagatctcat ccttaagaac aacagtcttg ctgctttctt ttccgttggt gttatatact
    29101 tttacttcca tctttccaga attacatatg agccattagg accgggaact gaacctttga
    29161 ttataatcag attctgttcc ggcattattt tcatgatctg caggttaatc atcttaactc
    29221 tgccattgcc atcgtggcca gccatgcgca ttcctttgaa tacccggctg ggatacgatg
    29281 atgcaccgat tgatccaggt gcacgaccgc ggttgtgctg accgtgtgtc tgctgattaa
    29341 cgcccttgaa tccatgacgt ttcacaacgc cctgaaatcc ttttcctttg gagaatccaa
    29401 caacatcaat atattcacct tcaataaata catctgcctt tacaagttca cccagatttt
    29461 tctgatgacc ttcctcaaag cgggaaaatt ccatcaaaat tttcttcggg gtggtgccgg
    29521 cctttttaaa atggcctttc attgcactcg aagtattttt ttcttttctc tcgtcgtaag
    29581 caagctggat ggcatcgtaa ccgtcagttt gcttggtctt gacctgagtc acgacacaag
    29641 gacctgcttc aataactgtg catgggacaa tcttcccaga ggcctcatga aagctggtca
    29701 ttccgatttt ttttccaatt aaacctgaca tgttattttt tttgttttag tagttacact
    29761 ttaatctcga tttccacacc acggggcaat tcgagtttca tcaactcatc gatggttttc
    29821 gactgctgat tgtagtaaat gtcgatcaat cttttgtatg taaaaagctg gaattgttcg
    29881 cgcgcttttt tgttaacaaa tgttgagcgg ttcacggtga agaacttttt atcggttggc
    29941 agaggaattg gtccgtttac aacggcattc gttgccttca caatcttcac aatcttctcg
    30001 gctgatttgt ccacgagata gtgatcgtac gatttgagtt taattctgat tttctggctc
    30061 actgtatatg atttttcagt ttgacatctt aattataagg tgacgcgaat acctttcatt
    30121 ttgaaaatga tatcctgcgc cagctcgttg ggggttatgc tgtagtgcga aaattccatg
    30181 ctcatgtttg cgcgtcctga agtaagactg cgcaaagagg taatgtatcc gaaagtttct
    30241 cccagaggta tgatggcttt caccgcggtt gaataaacct gtgcttcaat tcctgtgatc
    30301 tcacctctgc gacggttgat gtcaccggtc acgtctccga catactcttc cggtgtatca
    30361 acttcaagct tcatgatcgg ctcaagcaga ttgcatctga ttttgcgggt tgcttcctta
    30421 aaagcaatgg ttccgcagat ttcaaaagac aacgcatcgc tttcggacga catgaccgca
    30481 tcaaccagtt ccactttggc gcaataaaca ggatagcctg ccatcacacc tgtgcccata
    30541 gctgctttaa tgcctttctc aacggcagcc acaaattctt tcgacaatac gctggctttg
    30601 gtgctgttga caaattgcag tcctttctga tcgatgggtg ccggcgaaac cacaatttca
    30661 atttcagcaa agcggccacg gccacttgcc tgcttgcggt aggtctcgcg atgtgtgatt
    30721 gtaccaccga cagcttcgcg ataggcaacc tgcggttttc caagggtaca ctcgactttc
    30781 gattcacgtt tcaaacgatc gacaataata tcaaggtgca actctcccat tccactgatg
    30841 attgtctgac ccgtctcatc atcctgacga atggtgaaag tcggatcttc ttcagcaatt
    30901 ttctgaagtg atgcatttag tttatcgaga tcattctgcg tttttggttc aatggcaatg
    30961 ctgactaccg gttccgggaa atccatagat tcaaggacta tcggttgttt ttcatcacac
    31021 aatgtatcac cggtatgaat atttctaagt ccgatgattg cgcagatatc acctgcctca
    31081 acagactcca gaggattttg tttattggaa tgcatccgaa tcagacgcga tacgcgctct
    31141 ttctgctcgg tattactgtt atacaccata acacctgatt gcagtgagcc ggagtaaaca
    31201 cgggtaaagg ccagacgccc tacataaggg tcgttggcaa ttttaaaaac cagtgcacaa
    31261 aagggtgcat tcacagaatt gcttcttgac tctttggctt cggtttttgg attgattcct
    31321 tctatttctt ccatttcaac aggagacgga agataatgaa ttacaccatc aagaagagtc
    31381 tgaacgccct tatttttaaa agaagatccg caaaaaacag gaaaagccct gctttcgagc
    31441 gtggctttgc ggagttgctt tctaatatca ttggccgaga ttgaatccgg attgtccatg
    31501 taacgttcca gcaattccgg atcttcttca gccgctccct ctattaattt gagtctgtat
    31561 tcctccacga ttgaaagcat atcatcaggg acaggaattt ctgaaaactc ttttccaaga
    31621 ctatcatcat cccagacaaa tgctttgttt tcgatcagat cgaccacccc gataaaagaa
    31681 tcttcaactc caatcggaag ctgaactaca atgggattag ctctgagttt ttcgcgaatc
    31741 tgagcaacca ctcgcaaaaa atcggctccc tgacgatcca ttttattcac ataacaaata
    31801 cggggaacac gatagtggtt ggcctgtttc cagacagttt cgctttgcgg ctgaacagca
    31861 ccaacggcac agaaaatagc aatagcccca tcaagcaccc tgagcgaacg ctccacctca
    31921 acagtgaaat cgacatgacc cggagtatca atgacattaa cctgatactt ttcattattg
    31981 gaaacccaat acaccgtagt agcagccgaa gttatagtga ttccccgctc ctgttcctga
    32041 atcatccagt ccatggtagc cgcgccatca tcaacttcac cgatttcata attgattcct
    32101 gaataataca gaatacgctc cgtcgtcgtt gttttaccag cgtcgatgtg agccataatg
    32161 ccaatattcc gtatctgatt caggtctgtc atgaaaaagt ttagaatctg aaatgcgaga
    32221 aagccttatt ggcttcagcc attcggtgaa tatcttcttt acgtttgtaa gccgaacctt
    32281 cttctttgta agcagcgaga acttcagcag ccagtttgga ggccattgat ttttcgtgac
    32341 gcttgcgggc aaaagcaatc aggtttttca taccgatact gcttttacgc tccggacgga
    32401 tttcggaagg aatctgaaaa gtggcaccac caatacggcg gctgcgaact tcaacagaag
    32461 gtgttacatt ttcaagtgct tttttaaaca cttcgagtcc attctccttt gatttatcag
    32521 caatctgatc aatggcatcg taaaaaatct gatacgaagc gtttttcttt ccgctataca
    32581 tcaggttatt tacaaatttg gttaccaact gatcgccaaa ttttggatcc gggagaataa
    32641 ttctcttttt tggttttgat tttctcatat cgaaccgata ttattcaatt atgctttttt
    32701 cttcggtctt ttggctccgt atttcgagcg gcgctgcaaa cgtccttcaa ctcctgcagt
    32761 gtcaagcgct ccgcgtacga tgtgataacg aacacccgga aggtctttca ccctgccacc
    32821 acgcaccaat acgatgctgt gctcctgcag attgtgtcct tcaccaggaa tataggcagt
    32881 tacttcgaac tgattggtta aacgaaccct ggccactttg cgcatagccg aattcggctt
    32941 tttgggagtg gtagtataca cacgggtaca tacgccacga cgctgcgggc aggatgctaa
    33001 cgctggactc ttgctcttat actcaagttc cttgcggcct ttacgaacca attgttgtat
    33061 tgtcggcata caataacgat ttatttacag tttagttacc ccaatttttt tgggactgca
    33121 aaggtagata gtttatttca attaaccaac actttaagaa aaatattttt ttgagagtga
    33181 atttattaca aataaaaacc gattgactat tatataatgt ataaacattc tgaatgtcat
    33241 tcccatgtat tcgaaacgca ctgaaatatt gaaatcaact ctcaaacacc tgccgcaagc
    33301 cacatacccg aagtttatcc caacttccgc cgcagcgggg cagaaccgca ttcgccgcac
    33361 ctaccttatc aacatcatct taccaaactc cttggtaaaa tattggcctg ccggagtttc
    33421 aattttctcc atctgcagcc cgtcgagttt tacaataact cgatacaggt aaaccccatt
    33481 agccagctga tcgccgaatt catcgcggcc atcccatgca tattcagtga tgttgcggcc
    33541 aatatgaatc gggccaagct ccgacttgtc aatttccctg accacttttc ctgtcactgt
    33601 catgacctgg attttgaagt actccggaac aacagatccc gtaagtgtga atacaaaatg
    33661 cgtttttgtg ctgaacggat taggccagtt caaaacatcg gtgatggcgg gcttattgat
    33721 aacttcgaaa ttgatggaat aatcaatgct tccgctctca ttaccacttt tatcacgtgc
    33781 ctgcaccacc aacttgtaca ccccatcaga aaatgccccg gcaggaaatt caattctggc
    33841 cacattattc aatgtcgaag atgaggaata aaaccgcatg ttttcctgtc cgtcttcagt
    33901 aaagaaaacc cgttccagat cggtggcacc tggcttcaac aaataaatcc tgaatgctga
    33961 agtatcatcc agcatcagat atttgttctc gtctttcagc aaaatctcaa tcaacggctg
    34021 cgcacttaca atatccccat caagaatatg aatgccatca aaggttacat cgagcatggg
    34081 attgatgagg tccttaccta cggtaaattt aatttcagca atattgttga aatgatactg
    34141 ctccatctga tcgtaaaagc ctgtagcaga atcaaccgga ttgaattcaa cccaaagact
    34201 gttgctgcca gcaaatcctt cgctcgaaaa agaaatggag tcaacaagaa tatcacccac
    34261 agggtgcaga cgtagcttct tccatgcgat ttcatgaata ttacgctcat tatcaataag
    34321 ccaataggca acggaaagac tgtcgggaaa atcaaccgga ccaacattgc gggtggcaat
    34381 agccaaattc accatctcac cttcctgcaa tgtatcatta tagaatgtat aatatgaaac
    34441 cggatcaatg gctgtttcag gaacaggatc aaacagaacc tgccaacgtt caagctgcgc
    34501 cggagtgcgc aaactgtcat cactcatgtt catgtgcaga cgcagatacg gataaaccga
    34561 agcatcgatg cgggaactca ggttcagaat atcaagcgaa tccggaatgg gcggaaggtc
    34621 agcaattacg acatcttcgg tgccatcctg ctttatacca atcacagata atcgaacagt
    34681 atctgtccag atgccgggct caaaactctt cacgcgccag tgcagactgc cccactctgc
    34741 cgcagggcca atggtggttg attcaatata accttctttc caattggttg taatatccca
    34801 tgatccggaa ataatactgg tcaaccctgc cccaacacgt tcatcagcca tattcaggta
    34861 tcccttacga ccaaaaataa tatatggaac attattcggc aatgatcgga ttacattgga
    34921 accaatgctt tcaaatgctt cgtacaatga atcaggccag ttttgggcat tgtgattccg
    34981 gtggctgaat gcaagcacat aatatccgtc aggcacgcta tcaatcatgt tatacatgcg
    35041 cgaataccag ggttcgccgt aagtgaaaaa atcaaaatca taatatacat acgaacggca
    35101 gttcagcgca tcaaaaggtc cgagaccatc cagatcggga tcagtattgg ccattggtga
    35161 aatgctcaga gtgtcaaata tcgcaaactt catcccatca ccattgtaat tggtgcacga
    35221 ccattgccct ttaagaacat tgttgatttt ataccattct tctgaccaaa gaatatacgg
    35281 ataataaccg gtctgcgcct gaatgttgac gatggtattt ataaattcaa gaattctgga
    35341 tggcttgtta tatgaaacgt aattgaagtt gtctttcttg aattgaaaaa agtgcgcctg
    35401 cccccaacca cgttttccgg tgatatattg aaatgaactt tcacgccagt tactgtttga
    35461 agtcgaactg atacgccaga aatacacggt ggaatccggc atggacagca tgctgaacgg
    35521 taacgcccat tcaataatac ctccgctctg cgttatcgga ttgctctgca ccagaaaagg
    35581 actgtcgaaa gaatccgttg tatcaatctg aaatacatat gttcccgttg aagccattgc
    35641 atatccagtg ctggcaacca gcgtcacaaa agtatcgggc aaaacttcaa actcgtatgg
    35701 atacacagga ttaacatcgt tcgaaataat gaacagtgtt gtatttgttg aattatttgt
    35761 ttcatttgct tccgaaagtg catcaaaagc atcaaccata gccgagaaag aattcagccc
    35821 aacaccagtc agcgggctaa ctggcagcat aaatacaatg gtatcggcaa aagcagggct
    35881 tttaatcatt ttctgataca cttctgtagt gttatcagga taaattcttg tcagctccag
    35941 aataacggaa tcagaaacag ccataccaac attatgggta ataacattca ctgcaaaaga
    36001 atccagatcg ctggttacgt tggaagggtt aaaatatatg cttccggtag tcagcatcag
    36061 gtctggttgt ggttgtccgc caattctcat aacgggatcc ccgtgtaacg tcatttcaag
    36121 gcatacatca cggatatagg acgatgttgt ataactctga agcgttttca ccgtttccat
    36181 cattatgtaa ccaatgggtt tattgtaatc ctgcttcgaa attcgccgga tcagttcaga
    36241 actgtagctg tcaagcgaag tcatgagtga tttcgtaatt gagcccagat atccgatggt
    36301 gcctttgttt tcaatcagaa caaattcttc agctgaactg ttggtcggtt gataaagatc
    36361 tcctgcaaag caagaattgg caatcaaaaa tgggtaacgc ttgtagttgc tgtagtcctg
    36421 cggattatca atactgatat caaaaccgat tccggcggca tggccgaaga aattcagaat
    36481 ggccactcct tcattaatga gatctttcag actatcgctt tgattctgtt gaatcggtgc
    36541 tgttgatgat tttgaaaaag tcttgacatc ggctccgaaa tagggtgcct gaagtgtgtc
    36601 gcggtaatga ttcagataat tgagcagggt catcatctgt ccgtaatcag aaccaccggc
    36661 aaaatgcaaa acacgcttca tccattgatt ggcaaaagga ttggctggat tgaagggctg
    36721 attctgctct gtttcgaaat cctgcatttt ttcgagatac cattcaacag cagtggaatt
    36781 gcgggctgcg agccggccag tgggtatgtg ttgagcataa ttattgtcac caagttctga
    36841 tgtaaaaaga ttatctgaag gcggattgcc aaatgaaggc accagcgtca aaccattata
    36901 tgtaacgttt tttcgataat tgtactgagt atctccagcg cgatatccct ttccaatcaa
    36961 aaacaatcca tgtatggtat cggcaaaata agcacgcgca aaacgagtga aattggttat
    37021 cccaagcgga tgtttattga tgccgtatgc aaattcatca tacaacagat caacgtcggc
    37081 aacgagcgga ttatagcccg ttgttgagcg ataagaagca taattgttgg cttttgcaag
    37141 caacgaacga tgagtgacaa tcagataatc agtgccggaa tacgctgcat caccataatt
    37201 gataaaataa ccagaagtgc cactgctgtt caccggctgc attgatgaaa cataaaccac
    37261 attgctctcg gctgcaagat acatctgttt caaaccacca ctgttcggta ccaggaaatt
    37321 cacgctggca ccggcacgcg atgtcagaat acgacggtga ttggtcaggt catgaacaac
    37381 gaccgtatca gtagttggcg ctgtaagtcc tgtaaatgca aaataagttt ttgctcccgc
    37441 gccatcgtta gattcaaata acaaagaacc cgatgagttt agattcagtt gtcgcggata
    37501 acggatatcg atatagctca aagtggtaaa atcagctaca cttccaagat catcaggaag
    37561 agtgaaatta aaaacagttg aaccggatgt aaaagatccg gcaggcctgc ttcttacaat
    37621 ttttacgggg acataacctt caaaaatagt atcaattgtt aatccggcaa aattcatcat
    37681 cacatgatga tcgggataag cattgatgta atccgatgca ccaagcaaca ttaaatccac
    37741 agatgcatcg ggaccggatg caaataccga aggagtcgga acactgcggg tatatatcaa
    37801 cgacgaattg atatcaaaac cagtatcgaa ccaaccttcg caattggtat aacgtggatc
    37861 ggtgatgccg ttctgatcgg tctttccatt gaaataacgg gccgtgtagt ttgtccgtga
    37921 attgaaccac acataacttt cactggtgta acccgaaaaa ttcctatcag tttcaatact
    37981 tacacgccgg tttgttattg atgaattcca tgtcagaaag taaattgtcg tatcgttaaa
    38041 caagctgtaa tcaggatttg gcatatcggc cggattgtca tatacatctg catcaaaatc
    38101 gccacggttg cgctttccat agaattctat atagtcacct gaattgaaaa aaccatcatt
    38161 ttcgccatgc acataaatgt attgttcctc tccgaaagcg aacaactgga actgtcgcgg
    38221 gtcgaaggag gcaataggca ctcccgatga tgcaagcgtt gaataagaaa tcctgtaaat
    38281 accatcctga gcaactttta tcttaaaata gggctgcgaa taattaatcc attcattgcc
    38341 gtaattctgc gctttcacat cattacagca aaataatcca atgagaaaaa acagaaataa
    38401 taaattttta ttcattcatc agatacttgg gttaatactg cttcctgtgc ctttcggtct
    38461 gatattgaaa acaagtgaaa atacatgcga atacaaagca acactcacat cgcccagatc
    38521 ggtgagcgca taatcaatac tcatgatgtc ttttatgcgg attcccacac ccatattggg
    38581 ctgcaccgtg gtcacttttt ccccgtcgaa attggtttcc tgctgaatat tgccaaatcc
    38641 gccacgcaga tatgccacat ttttataatt caattcaaaa ccagcatgca gatcggcact
    38701 ggcaaaaccg gttttcaata tcacatttct tttcccatca aaagtaatgt ccataccggc
    38761 ttcgggatat atggaaaagc tgtttgaaat atcaaatttc cttcccgcac ccagcagcaa
    38821 tcgaggcatg gtgagctcca gactgttttc gggaatttcg tttccggtga gcgtaaacac
    38881 ttcgcgggtt gcatcatcca acgtaaatga ccaggcattg aatgttgaag taacatcgcg
    38941 caataccgcg ccgaaacgcc aattgtcctt tctgtactga gcgcctgcat caataccaaa
    39001 gccccagctg ccgccgaaat cgcccactgt gcggcgaatg attttcaggt ttccaccgac
    39061 ctgcaggttt ttgatgggca gcttgcgcga ataactcacg ataaaagcat tgtcgatggc
    39121 actgaatgtt gtaatgcggt catagtctat gtttccctgg gcatcaatca gctcggtggt
    39181 attgggaata tcatcaactc caaaacggat aaacgaaaaa gcaagtgccg aagaatcgct
    39241 cgtgaccgca gccagcgaaa tattgtcgta tttggctatg cccgcaaaat attccgaatg
    39301 catcagaaaa agctgacgcg gagcttcgca atcgaccaat ccggccggat tccagtaacc
    39361 cgccgaagca tcgccaacag aagcgactac cgaattcgac atgcccaaag cccgcccgcc
    39421 aacaccgatg gcaagaaatt cattggaata cttgggcgcc tgagcaaaaa cggaaataac
    39481 caaaaaaaac agaacaagaa ctgcaataaa tttattcatt ttatctttac ttcaactttg
    39541 agtgaataaa cgcgctctgg tttaaaaaag tatgtggttt ttttaaattt tccacaccac
    39601 atcgccgctg ccgcatgaat gcgcaagact gcgtgccagc acaaagaaat aatcgctcag
    39661 ccggttgata tatttcaata tttcaggatg aaccggctct gtttcataca aacgtaaaat
    39721 ggtgcgttcc gctctgcggc atacagtgcg gcaaatatga gccatggccg ctttctcgca
    39781 acccgccgga agaataaaat tggccaatgg cggcaatgcc gcagtcagcc ggtcaatttc
    39841 cttctcaata gcttccgtat cagaagccaa caattccggg aaatacttct tactcccgga
    39901 cacaggatcc acagccagat gcgactcaat cagaaacaag cggttcatca cctcaaaaag
    39961 aaaatttcgt gtgtcgccgg cttccacttt ttcgtacaga caaccggtga aggaattcag
    40021 ctcatccacc attccgtagg actcaacacg aatgcaggat ttcgaaacgt tctttccgcc
    40081 aatcagcgag gtggtgccgc tgtcgccttt cttagtgtag attttgcctt ccatagccat
    40141 ttgcagtttt attcgtgttc tttcagtgat tcaagcactt cctgataact ggtaaccttc
    40201 gggtttttct cgtcggtttc gaccagcccg tcgcgcagtc tcacaatgcg atgcgcatac
    40261 cttgcaatgt cttcttcatg cgtgacaaca ataattgtat ttcctgtttt atgcaactca
    40321 tccagcagcc ccatgatctc caccgatgtg cgtgtatcaa ggtttccggt gggttcgtcg
    40381 gccagaatga tggatggatt attcaccagt gcacgtgcaa tggccactcg ctggcgctgt
    40441 ccgccggaaa gctcattggg cttgtgttcc atgcgatccg acaatcctac atcggtgagt
    40501 gatttttcgg cgcgttccat tcgggcttct ttgcggatac cggcataaat caacggcaac
    40561 gccacattgt cgagcgccgt gtatcgcgga agtaaattga aagtctgaaa cacaaaaccg
    40621 atttccttgt tgcggatttc agccagttga ttgtcatcaa gctggctcac atcctgattg
    40681 ttcagaatat aggtgccgcc ggttggcgta tcaaggcagc ccagaatatt catcaatgtt
    40741 gattttccag agcccgaagg ccccatcagt gccacatatt cgccacgctc aatattaaat
    40801 gaaacgctgt tgagcgcctt cacaagcacg gtcccgactt tataatggcg gctgatgttt
    40861 tccagcctga taacttccgg catgatgaaa tatttttaca aagctaatgg attttttgat
    40921 tgaaaaaacc aacaatcaat tgagctttac ctacaccctc aaccgaaaac actccaccga
    40981 cagatccgca tttttaataa gcaacccgaa gcggaaaaaa tccattttca cctgaaaggc
    41041 cgggtgctgc tgaagctcgc tccagcattg actcatttcg cgggaataat aaatatcgtc
    41101 aatcaccaca acggcttttt cactcagcgc aggcagaatc atttcaagat attcaagcgt
    41161 cggtttgcgg cagtggtggc cgtcgatgaa caccaaccca aggtcttttt cctctgcaac
    41221 aatctgtggc atagtttcgg agaatcgacc tgttttcaaa ataacatttt cagcttcgta
    41281 tttttcaaaa tgttccttcg caatggcagc aatttctgga acgccttcca gagtaatgat
    41341 gcggcttttg tgtgctgcaa aatgcatagc cagcgttgtt gtaccaaacg cggttcccat
    41401 ttccagaatg gtctttccgt tgacatgcgc tgccagttcg gcaatcattt ccgcttcgcc
    41461 aatagaagtt gaggtccttt tgaaaacctc aaaaacagta gtttctaagc gcccttcacg
    41521 cgatcccaat tcattaaaca caagcggcgt tttgtcaacg ctaagctgtc catgcaggaa
    41581 tgcaagcttt gtaatcgcag attccttcag ggctttcgga taacataatg aatggtatat
    41641 ttcgtataac cagggagaat gtacgctccg aagtcccctg gcctgaaaga aatgtcgcat
    41701 tgctgcaatg atcctactca taaaccaata ttaagaattc tcgtccttat cgagtattga
    41761 taactgaatc cgcaacgatt catcgtgaat gacctgcaac aattcacgga taaattcatc
    41821 attgagatca agtgctgaag cccgtttcag acgatcctcc accagacttt tccagcggcc
    41881 tccctgcatg atggtcatgc cggcatctcg cttcacaata cccatgcgtt ttacgatgtc
    41941 aattcgttgg gcgagaattc gcaaaagccg ttcatcggtt tcatcaattt cgcgccgtaa
    42001 tgcctcgagc gaagcatcgt gcaacgatgt cgactgcttg ccacacagac tatcaatcag
    42061 gaaaacaaat tcagccggag tgatctgctg gttggcatcg gacaaagcgc tgggcggatc
    42121 tgcatgcact tcgatcataa gaccatctgc ttccatttct ttggcttgtg cggccagacc
    42181 ggccacataa aacgaattgc ccgccatatg acttggatct gcaataattt ttatttccgg
    42241 ataacgggtc cgcatttcgg ccatcaacac ccagagcggc tcgttacgga atttgctttc
    42301 attgtatgtt acaaaaccac ggtgaataag cgcttcaacg ttcaatccct tgttcagcag
    42361 gcgatcgacc gcaccggccc aaagccgtac atcgggcacc agcggatttt ttacaagaat
    42421 gcggatattg cttccctgta gcgcgttggc cagttcctgt acactgaaag gatcggttac
    42481 tgtgcgggca cctacccaga tggcatcgag gccggctttc agaacagctt caacatgcgc
    42541 tgtggtagcg gcttcggtaa ctaccggcaa tttgtattga tccctgaccg cgagcaaata
    42601 tttcagaccg tcgttgccgg ggccttcgta cataccgcgc aacgtgcgag gtttccatac
    42661 tcctccgcga aacagatcaa cgtaacctgt acgcgcaagt ccggccgcaa catcaaggca
    42721 gagtttctcg gattcggcac tgcatggacc tgctacaatc atttttaatc caggtgctga
    42781 catgactatc ggtttttaca aagatacggc atttgaacca ccgtttccaa ccaaaacaac
    42841 aatttttttg agaaatcttt ctgaaagggc ttccctcgta agccatcgaa gggttttgaa
    42901 cccttcgatg gctatatgcc cgaaatcgct ttcattcccg gcatagaaac aatccccaga
    42961 tgcttcttcc cgtccacaag aatttccatc ggctgatcga accgcacatg acggataaca
    43021 ccattgtcga acaacgcttc ctgagcgttg agaaatgcag tattgtacat tccttgttta
    43081 ataaaatcat tcacggtgaa ataggccaca cggaacgaag tgagattctg aaaaaaatgc
    43141 gttccctgac tgggttctat gtgaaaatga ttcattccgg cctcgacaat cacgcgtgca
    43201 gcactgatct gcgaccactt caccggaatg ccgagccatg gatcactcga tccccaccga
    43261 cccggaccaa tcaacagata attccttttt tcattggaca gcgatttgtt gagttgatct
    43321 ataacctgcg ccaggtgcag ggtatcagca gcattgaact tatcgggaac cacatacacc
    43381 acatcgcaga tattgtcgta aactccgttt ccaagcgccg attcagaaat aataatacaa
    43441 gcatcatgtc tgatatgttc aggattaatg gtcagattct ccttattatc aacaattggt
    43501 cttatctgca gtaaattaaa aactttatga tgccccagtc catccatatt gacggcgaat
    43561 tcaatttcaa caggtttccc catttcacgc gagctggttt caagaatatc cgtcatcatt
    43621 tcggcgagag gaaacacatt atgttttaag aaatgattga aagtgattat ccgcattcct
    43681 ttctgactga ttccatcaac cacccgatca tcgtgataga cataggttga agcagccatc
    43741 cggaaagttt taaactgatc accatcgctc actgaaaagc gctcgatatg tgcattatca
    43801 tcaactttag gggtaaacag gtttcgtttg agattgatgg caaaaaactc tttctgtgta
    43861 tctttaagca tcatttcagt cgagctgagc tgaatagctt tttcaggaaa acgcggcgaa
    43921 aaccgcaaag acattccacc atcaacaata tactttccca gaccaaatgc aatgcttaca
    43981 acaccgtctt cggccttctc gggactgatg gggtaaaaat tgacggaacg cgcaacacca
    44041 ctcaaaacag gaaaataata atctccttcg ttcagtccac atacttcttg cagaataatg
    44101 gccatttttt cttcgtcaat cagattcgaa gtagatgtca tatatgcctt gctttcctga
    44161 taatacaccg aagcatacac ggccttgatg cattgcatga gcattttcaa acgatcgcgg
    44221 tcgtcgcccg tgttcggaat catatacgtg ctataaatgc ctgcaaatgg ctgataatga
    44281 ctatcttcga gcagcgagct tgatcgtacc gccagtggtt ttttcatcac ccgtaaaatg
    44341 gatttcagat caagaattgt ttcataattc aattctgctt cgagaaaacg gcttaaaatt
    44401 tcttcatcat taccggaagc aatagcaaaa tcatacaaat cgtttcgctc cataaattcg
    44461 ctgaacacat cggtagccag tacaaccgtt cggggaatgg tcagttcgac actgccaaaa
    44521 cgatgagcca gctgattgcg ttttaaaata ttatctatga aagccagtcc gcgtgctttt
    44581 ccaccaatgc tgccctcacc cacccggctg aacaaaatat attcattata cttgtttcta
    44641 tcgaaagtag caataacgcc gcgtccacgc actccacgat actttgccat tcctttgtac
    44701 aaataatcac gcacttcatc cagtgaagca aaatcctttg aattggcctg tctgaatagt
    44761 tttccaagcg aaaacaaagc acgggcgcgc agccatttcg aaaaatgatt gcgatcgata
    44821 tgatatttca aagactcatc aggaatgctg taaatctgtt cctgcaaact ctgcaaatcg
    44881 cgggcgcggg ccacttcttc gccgttggcc ggattcacga ttctaaaatc gccaaaagcc
    44941 agataatcca acaaaaaatc ggtaagttca tgcaacaggg tcttggaaaa tttatgcaca
    45001 aacttcacct tgatatcatt gatcagatcc tgggtaatga catctgaaga ctgtaacaaa
    45061 acaggaaaat acggatcatc ggcctgcaca atccgactga gttcaattcc ggcattttta
    45121 tcttttatat tgttgcggtt aaacgaaata tccgaaatca tccccaacat gtttgatttg
    45181 tacttttcat acaaactcaa cgcctgttca aaattggttg ccagcagaat ctttggtctg
    45241 ccgcgcaaac gcaatgtacg ttgatgttcg ttcaaccctt cactcataaa tgcgcgcgat
    45301 tgctgaataa caatacgata catgttgggc aggtaagaac tgtaataacg caccgaatcc
    45361 tcaaccaaca aaatacactg aacaccgacc tcacccacat caaaatcggt attcatttta
    45421 tcttcgatca gtttgatgat ggcaagtaaa agattggtat tgcccagcca actgaacaca
    45481 taatcaacgc cactcagatc ctgtactgca agtcgctgag acacttcacg ggagaacgga
    45541 gttaacacta caaccggaat attggcaaaa cgttgtttaa taacttttga aaattcaaat
    45601 ggattctgat ccgaaatact gagcatattg ataacaagat ccacccgatc gttttccaga
    45661 atctcaagtg catgttccgc gctatgggct ttaaagaatt ttggaggata ccgcagattg
    45721 agcgaaacat attcctgaaa aatagcttcg ttaatgcgac catcttcttc aaggataaac
    45781 gcgtcataag tgctgcagat aagcaatata tgctcaatcc gcctttccat cagatgttca
    45841 aacaaagtat cctgaaattg cgcctgattc aggtccggct tgttgtgctg tttggtcata
    45901 ggcatcaatc tgaactaaaa ataactgttt ttgaaaatga ctgctctaaa gtacaacatt
    45961 taatggccgg tctgaaatca ttgcggaaat ttttgtgtat ttgaatcgtt gaaatgacta
    46021 catttgaatc tgaaaaacag aaaaagaaaa caaaattcag taatcaaacg tgtgtaatag
    46081 tttcagttaa ctttgcagca gtggagaaaa acaaaaaaaa atctatcaag gaatgggcgg
    46141 ccgatgatcg tccccgggaa aagatgcttg ccaaaggccc cgaagcactc agcaatgctg
    46201 agctgtttgc cattctgatt ggaagcggta caaccaaaaa aagtgccctt gaactagcca
    46261 gggaaatgct ggaaaatgcc gataacagtc ttaaaaaatt atcgactctg accattcatg
    46321 aaataaaaaa aattcatgga ataggtccgg ccaaggcagt caccgtagct gcagcactcg
    46381 aaattgcgcg aaggcgtgaa ggagaaagca ttgacataga agaacttgtt ttatcatcac
    46441 gagatgcaca aaaagccctc ctgcccatgc tttgcgacaa acaacatgaa gaattctgta
    46501 tcatggggct gaaccgtcga aacaaaatca ttttcaaaga aagaatcagc atgggcagca
    46561 tggttgctac tcaggttgat atacggaaaa taatcaaaat ggctctggac acacgatcac
    46621 taagtcttat ccttggacat aatcatcctt cgggaaatct caatccaagc gattcggata
    46681 taaacctcac ccgacagatt tatgaagcat cggcattatt cgatatccga ttgcttgacc
    46741 atatcatcat atcacaggat tcctactaca gctttatgga tgaaggaatt ctcaatactt
    46801 tcaaaaaatg aaaatcgtaa ccattgttgg cgcaaggcca caaattatta aagcagctgc
    46861 gctgagtcgc gaaattcaaa atcattttaa tgatgaaata aaagaattca tcattcacac
    46921 aggacaacat tatgacgata acatgtctga agtttttttt cgcgaactca gcattcctgc
    46981 accaaatgta aatctcaata ttggttcttc cagccacggt gaacaaaccg gccgcatgat
    47041 ggaaggcatt gaaaaagttt tgactgaaaa caaacccgat gcacttgtaa tttacggtga
    47101 taccaacagc acactggcag gcgctgtggc cgcagcaaag atgcacatac ccgtttttca
    47161 catcgaagcc ggattacgtt ccttcaataa aaaaatgcca gaagaaataa acagaattgt
    47221 ggctgatcat tgctccacac tgatgttctg cccaaccaaa acagccatac aaaatcttga
    47281 aaatgaagga ttcccggcag gcctgatgag cccctattca atggataaac ccggcgtttt
    47341 tcacgctggc gatatcatgt tcgataactc cgttttcttt gcccaaatcg ccgaaaaaca
    47401 atcgaaaatt attgaagatc agaatctgaa agatattccg ttcattctcg ccacggttca
    47461 ccgcaacgac aacacagaca acgaagacag actgcgaatg attttcgacg ctttgtgcct
    47521 tgcatccatc aacaccaaat taccggttgt tttcccgctt cacccacgca caaaaaaaat
    47581 gatggatgca tggaacatcg gcagcaatat ccctgcagga gccgatgttc gaatcattcc
    47641 accggcatct tttcttgata tgattctgct tgaaaaaaac tgtcgtctcg tacttactga
    47701 ttcaggtggc gttcagaaag aagcatattt cttttctcgc ccatccatca ttctgcgacc
    47761 cgaaaccgaa tgggtcgaaa tagttgatct gcgcgcagca aagctggccg atcattcagc
    47821 tccggaaatt gccgctcagg ccgaagccat gatcaattat ccacccaccg aatttccacc
    47881 cgtatttggc gatggtcatg ctgcagaatt tatctgcgaa aaaatgctgg aattttatca
    47941 atccttgttg cgaaaaaatg caataaaaaa ataatgagca ttacgattct gacatctttt
    48001 gacaacgcca aactgcgcta caccctcgat tatatcttcg atgaacggct tggctgcgga
    48061 tatgtactga atcctgaaaa catcaaaaac aatacggcca ttgtttctta tggtacaaat
    48121 cataatcagg gactactgat ttcagacagt aatttccttc acccaaaagg cgtttcggct
    48181 caaaaagcgg aagtcctcat tgatgataaa aataatattt gcctgttccc tgcagaagaa
    48241 tcagctgatt tttcatttga tgtttttgct gccatatttt ttatgctgag tcgctacgaa
    48301 gaatacacca gtcttgaacg cgatcagcat ggacgtttcg atttgcaaca ctccctcgct
    48361 tataaaaaca attttcatct ccgagccgtt gtcgatgaat ggattattat actccgaagc
    48421 cggcttctga agcgcttttc ctctgttact ttcaaaaaag aatcgccatc ttttgctatc
    48481 acagtcgatg ttgacatgtt gttctcctac cgcacaaaag gatttctgcg caacatggcc
    48541 ggctgggcac gcgatcttgt caaaggcaaa ttccaggcgc tttttgaaag gccccttgtt
    48601 ttacttggtg tgcgaaaaga ccccttcgat acttttgata tcatcgataa aatcagtcgc
    48661 gaatttcatg taccaatact attttttgtt cttgcttctc agcaaagaac aaactttgac
    48721 aaaaacggga acctgcacca tcaaagagct gtgaaacaat tgaaaaaaat atcacgtgaa
    48781 tcactgatag gacttcaccc ctcttattat tgccttgatg acgataacaa actgataaag
    48841 gaaaaacaaa atctcgaaaa aaccattggg acatctgtca cagccgcacg ccggcatttt
    48901 ctgagaatga atctgcctca atcgtaccgg caactaatca aagccggcat tacaaacgac
    48961 tacaccatgg ggtttgcttc aggccatgga ttcagagccg gaaccactcg ccctttcaga
    49021 tttttcgata ttgaatcaga catcgtgttg cccctgattg tacatccttt ctgcatcatg
    49081 gacggcagtc tgaaggatta tcaacaaatg acacaggaag aatcacacag agaaatgctt
    49141 catctagccg aatatataca atctatcaat ggacatttcg aaatgctgct gcacaacgaa
    49201 acgctctctg aaagcggccg atggaagggc tggacgcaaa tcatcaaaga aaccatccgt
    49261 caatttcaat catagcaaaa atgatacgtt ttataaaaaa cgaaagtatc agcagagatt
    49321 tatgggataa agcaatcatg cagtctccgc aaccaatgat gtatgccatg tcctggtatc
    49381 tcgatctggt gacacccggc tgggatggcc tgattgaaga taattatcaa gcggtaatgc
    49441 cgcttattgg tgcaaaaaaa tttggaattc actatttgta tcagccccca ttctgccagc
    49501 aacttggtgt ttttggaaaa aatacttcga ccgatgttgt aaaaagcttt ctgctggcca
    49561 ttccgcgtaa atatcggttt gccgaaatca tgatgaacga atcaaataaa atcgacttga
    49621 ccaacgctga aatgctcact aatattactc ttcagcttgg ttgtgctatt gaaaacatca
    49681 gaaccggata caacgaaaat acccggcgca acatacgaaa agcagaacag aacaaacttt
    49741 cagtttacaa aggatttgat attgaaaaca tcatttcact tttcaaaacc aataaaggtg
    49801 aagcgctcgg caaaaacgaa agctggtatt caacactgaa aacgctgtcc tatcagctac
    49861 gtcaccttag tctggccaac actttttgcg caatgaacca acacaacgaa atcattgcag
    49921 gcatcctgct cgttgaattc aatgggcgtg cagtactgtt gttcagcggc tccggtagcg
    49981 aagcgcgtga aaccggcgcg atgcatttcc tggtcgattc aattctgaaa gacatatgca
    50041 cctcgcacca gatcttcgac ttcgagggaa gcaacaacga aaatctcgcg aagttttatc
    50101 tcggattcgg cggaaaaaaa tcctcctatc cgtttgtgcg gattaatcga ttgccttttc
    50161 ctgtgcggtg gatgaaaggg aaaggtcttg atcattcaca aatttcagcc aaatgaaact
    50221 acttgatgtg ttcgataaag accctgataa ccaaacaggt tgcagtaata ttctgatgaa
    50281 gtgtttattc tcttttgtaa ttacatatct tattcttttg atcttttcat tagtatcgaa
    50341 taacaaagag agccaacaat ggttgccaat cattttatta attgtaaacc caattatttg
    50401 tattattaat agcatttata tgtttttact ttttgcgttg ggttttgata aaggtgggat
    50461 ttccggcatg ttgtttattc tgattgagag catatttctt attgcaatat attcaggaat
    50521 tagccttgtt gtaaccaagt tgtctgtaaa tgtattgtac gaaattgaaa atggctcaat
    50581 gttgagaaga aatgtctttt cagagcattt tcaactgctt ttcacatact tcctgctgac
    50641 aatatttctt gttgcacttt ggaaaatatt aaaaaaacta cctgagtgcc gctgtcaatc
    50701 cattgcccta cttttgtcat cctgagccct gctgctgtta ctctgaaccc cgccgctgtc
    50761 atgctgagcc cagcctgctg tcactctgag cccagcgttg cgcaggataa actccgtcga
    50821 aacgttggca gctgggtaaa tccgtcaaag tgtgaattgc gggactcagg atggcggcca
    50881 gttgccgaaa aaatcataaa aaatttgcgg aatacaaaaa tagtatatac ttttgtaata
    50941 taccattaaa gtacatcatg aaaacacttt cattaaaact ggatgagaat atttttctgg
    51001 aaacggaaaa agtattatct gaaataaaaa agccacgcaa ccgctacatc aacgaagcgc
    51061 tgaaattcta caacgcactt cagaaaagac gtttgttgtc aaagaaatta aaagccgaat
    51121 cgggattggt ggccgaagag tccatgaata tattgtacga attcgaaaac ttcgaagacg
    51181 atgaagatta agcagttcga tatctggatt gccaatctgg atccgcgttt tggcacagaa
    51241 gccgggaaat cacgacctgt tctgatagta cagtccaacc ttctgaatga tattcatcca
    51301 tcaactttga tttgtccgat aacgaccaat gttcagccaa aggcagaaat actgcgtgtt
    51361 catttaaaga aaggtcattt cggacttaaa cagaattgcg acgtaatgat tgatcagatc
    51421 agagccatcg ataacaaacg tctgatgaaa cgcgttggcc ggatttccga ttctctgatt
    51481 gaaaaagtga aggagaattc tgccattgtg cttgatttga caaaaacctg aataacatga
    51541 aaaaagaggc gcagtctccc acgcctctta ccatcttacc aaatcataaa cagataatta
    51601 ttttctgatc tcaagctcct cgatgagcct tgttccaccg gcatatttgt caatataaaa
    51661 aagtacatac cggatgtcaa cacaaataca acgctgcagt tcaggttcaa acacaatatc
    51721 gcccgacatg gcttcccagt ttgcatcgaa tgcaagacca atgagttcgc catttccgtt
    51781 aagcaccgga gagccactgt ttccaccggt tatatcattg tttgtcagaa aacaagtaat
    51841 cattttacca ttttcgcccc actgtccaaa atctttcttt tcatacagtt ctttcaatct
    51901 ggctggcaca ataaattcat cattatcagg attttctttt tccatcacac cattgagggt
    51961 tgttacataa tcataatcaa tggcatcgga tgcactataa gacaacactt ttccgtaagt
    52021 caggcgcaga gtgctattgg catcgggata aaaatgtttt tcaggatgca tttcgcgcag
    52081 accctgcaca tataagcgca ttccctgatt cagctgataa ttgagcggat tgatttcgcc
    52141 caccaatttg ttataaattt cataaacctg tatagctgcc tcatataatg gatctttgag
    52201 caagacatcc tttgtcgggt tttccaaaaa agcatccatt ttgtctttgt ttgaaaaaac
    52261 cgaaaccaaa aaggcaaaat ccgaatagag ggcataatcg tttttaaact tattgctgat
    52321 gatgctgaaa aacgccggat gatattcggc aggaacatcg gctgcataca tagcagtcat
    52381 agcactgaac aactgcattt cggtcttaaa atcaaatgtt acgaaatggt tttccgaaac
    52441 tcttttcaaa tacgaaacca tatcatcaat ctttgtcaga ttgggcttat cggaagacaa
    52501 ttcagccata agagaccgga attttccggc aaaagaaata acatcgctgc cacggaaaaa
    52561 tgcttctgaa atataatact gcactttacg cagagagctc agcttttcaa tagaattatg
    52621 ggttttgatc aatgcgtttt tataataacc aaactttgga atatcatcgt tattcaccca
    52681 agtttcaaaa tcatcttcaa ggattttttt tcgttcaatg acattgttac gttcaatccc
    52741 tttattctgc cctatgtaat atttccagta attagcagtg gttgcatatt tgctggcata
    52801 tcgcagtcta acctcatcac tggctttcat attggcatcc atgagagcaa gtttcttttc
    52861 gcgtatttta acgatggaag gatttacata aaggtaattt gacgcaactt cttcactggt
    52921 gatatagcgc gatgtgcgac caggataacc aaacaccatg gcaaaatcat tttccgtttt
    52981 tggtttgatt gaaaccggca gaaaatgttt cggtttcata gggatattgt ttttgctgta
    53041 tttggctggt tttccatcag gaccggtata aaccctgaat acactgaaat cgcccgtatg
    53101 acgcggccac atccaattgt cagtatcgcc cccgaaatta ccgattgaag atggcggatt
    53161 caataccagt cgcacatcgg aatagacttc atatacaaaa agataatact cgttgccata
    53221 aaaaaacggc tttacattgg cctcctgata gctttcagaa acagcatttt tttcaagacg
    53281 tttcgatatc cgacccacaa tttcatcacg ctccatttcg gtagcttcgt acggaatcga
    53341 atccagaatc tgctttgtca catcctcgat acgggccaga aaacggacat tgagattgga
    53401 tgccggaaac aattcatcgg ccattgttga cgcataaaaa ccattggtca aaatgtcatt
    53461 atccactgta ctcaacgaag caatggaagc ataaccacaa tgatgattgg taaatatcaa
    53521 tccctgatcc gatacaatct cgcctgtaca gccaccacca aaaataacaa tagcatcttt
    53581 cagacttccg ttgttgaccg aatagatttc ttcaggggtc agatgaagtc ccattttctg
    53641 catgtcggta taattcaacc gatcgataaa catcggcaac cacatgcctt catcggccct
    53701 ggattgaaaa aacaatgaaa caaaaagaag aaccccggca attttaatcc tgtttttcat
    53761 aagtcagata atggtgcatt ttcgtttcgc aatattataa catttttttc acttcaagcc
    53821 attccaaagc caatttgcgt gttattttcg acaaacaaaa cgaatttaaa aacataacct
    53881 atattattat tgctatgcac tttgcatttt tgggacaaaa acaagaatgc cgcttcatgt
    53941 aatccataaa gcggcatccg aaatgatcaa ccaacgcctt tattctgcgc cagccgtcat
    54001 aatcatccgc gaaatactac cttccgatgt tttcacccgg atataataaa cacctccgct
    54061 caaaactcct gcatcaatgg ctgtacggct gaaccctttt ggcatagtgc cactgaacaa
    54121 tacttccagc aaacgtccgg tattgtcaag aagttcaata ctcacattat cagcagttcc
    54181 caattctata tcaacaaaca tcgattcaac tgcaggattt ggatatacat taaaactaaa
    54241 ggtaggttcg tccaatcctg tgtgatttgc caatgcagga aacacaatat aatcaaccca
    54301 tgcagcatca tcaccatcgg cggcagtata atccttcaga tacttccatg taaatgtatg
    54361 ctgacctgaa gttacaggat acgctgcccg cgaccagccc tgttctccat cccaccaatc
    54421 tttgcttgag gcatcaatca gaaattcaag ataatcccat ttctgactgt atagacttcc
    54481 attctcacaa cttactttat aataaaacga aatagtgtca ttcgatgcca catcccattc
    54541 aaccgacatg gtagatgtct gctcattggc aatatcgcct gactgcgaac aatatacacc
    54601 ttcgtatgga ttattggtcg atacaaacca gggtgcattg cctccggtag tccatgcata
    54661 ttgggtcaga tcgccagtct cataatcttc gtcaactatt ccaaccttca ttgtaaatac
    54721 tttttcaacc tgataaaaac ctgttcccag cgtataggtc aatgatacag ccgaacccag
    54781 agtagcgcca ggatcaaccg aaatactaaa gccggcatca tgtgttaaag caggaatcat
    54841 gtcccctata ttatcggttc cattgacaat gctaatgcca gaacttacgc ttgacaaaac
    54901 tcctgttgca gccggataca tggcatgtcc gttgttcaaa gaagcaatca gaatgttgac
    54961 ggtttcaccc ggatcgagtt tgccattgtt gttgccagct gcatcatcaa tcacaagccc
    55021 tcctatgcta agtgaaggcg cattaattgt cattccgaag gatgagttcc atgtatcggc
    55081 cccatctaca gtcgtcaatc caaacaaaac actggtttga tcaacaacat cactgcctac
    55141 gcttacggca aaggcattgt tcattggctt ggtggctccg gcgttgatgg caccataagt
    55201 ttcagtatta tccgtaatgt ttatcgccgg gtttgtagtt gaaatagtag caattacatt
    55261 agctgcattg gcaactccga cattattcaa tgttacattc aaaaggatgt cctctccaaa
    55321 atccgcctga gagttattgt tttcgctggc atcgttaata ctgaatgtac tgtaaatcac
    55381 atatggaccc gtagctgcta ttaccggcac ttcgcctgta tagggaaaat aattgtattt
    55441 ggtggcagtt acaaccagtg tgtcaatgga tgagagtgcg gtaaagctga tggtagcaac
    55501 gcccccagac acaattccgg taccgattat accatcattc aatgtaaccg taacaaatgc
    55561 atcttcagta ttgcagttta caaccaattg agcagtgcca attggaaccg tggcaagatg
    55621 tgttaccgtc atggttttgg gcgtatcggt tctgatcatc agcgaaggat cgccaaatat
    55681 agtccaggta tcggtcattt catagccatc gctgccatag ctgtcgttca ttttcatgca
    55741 accgctcatg acaattccac caaatgtgtg tttgatattc ccggctgaag tttcgcacaa
    55801 aacattcacc atctcatcct gaccttccat gggcggagcc caatcctgat tgatagtgct
    55861 cataatcgtt gccacagcgc ctttgggtgt tgtaccattg cgggcacgca gccatgcctc
    55921 ggcaaaacaa gtctgtccga caaaattacc attgacacaa gcaacagaaa tgataaaagg
    55981 ccattgtgtt gtatttgtga gattgccaac atcggtattg ttaaaatctg tagtactgaa
    56041 tgaaatatca ctaccatgac ccgtatatag caaaagaccg gccccggcat ttaaaacggt
    56101 tgaaacattg gcagcagtcg gatcacctgc cgcatccaat ccgccctgag agccatcgaa
    56161 caattcatat ttggtttcat attcataatt gtcgcactga gtctgcaagt tgcggatgtg
    56221 ctcataatca tactcgcctt catcaccagg accctgactg gaagcgatac cgagcaccct
    56281 gttcaaccag ttgttgctgg tgtttggatt ctgctcatac gtaagggtac gcaaaacctg
    56341 cacatcaaca tctcctgtgc tttcagctga aaaacgacct acataaaatt caggataatg
    56401 gtcactgccc gtctggtagg cataggcatt gtctgaatgt cctgccacac cgctcgttat
    56461 tggtggtata tcacctccat ccccaacaaa taaaagaaat gtcaacccgt ttgtgttata
    56521 ataatttgtc acataagttt taatggctgt gctggtggtc ccggtcgttg tcgtactgac
    56581 aatttctgtc ggaaaaccaa ttctgttttt ccattgaaca tatggtgcca tattggccat
    56641 gtattctggc ttacagacca ccagaattct tccgcgctca gtaatcggag tgtatttcac
    56701 ctgactgtaa ttgatgaaat gcctgttgta taattcatca aaactctcat caacttttga
    56761 aagagttttt gtgcggttga atgcattcaa tgccgaatta tctgaatctt tgtaaacaac
    56821 gatgcgtatg gttttgacta cacgcagcga acgaacggca tgatcgtaac gaaacggata
    56881 cacatgcagc gccactcccc tgtaatcgcg catgatatat ggatcggaaa gactggcaaa
    56941 agcgcctggg aaactgccat tctgctgata ggtacgtccc cacgtgtacg caacactact
    57001 cggatcaata tctctgctca gactcccttt cgaaggaata atttcaacat tgctgaaatc
    57061 gacataatca gctgaaagta cttttacctt catatcagct gcatcgggaa ctatcagcga
    57121 attcgaaaaa cgacacaaag ccggctcacc tgctgtgagt aatggagaag caccgtccat
    57181 actgactttt tgtttcacac cctgcgatgt ttgcacatca ctcatgtaaa aaccctgaaa
    57241 attgtattca ataacagttt catcaggcgt tgacgaaatg acggtgtaca atggtttacc
    57301 agcatcgttg ttgagcgaat gataggtctg gctactcatg gagcccatca acaccatcat
    57361 cagaaatgaa aaaataactg ttttcatgcg ttgggggatt atttgattac tgcaaaagat
    57421 ctggaaaaat taacctgatc accattcaga tgaacagtat aaataccagg ttcccattca
    57481 taaacagaaa ccggaagata tagacgacca cttatattgt gataattttc ggaataaacc
    57541 aaacgaccat ccgatgcaac aatcgtcaac tgaacatcgc ctgatataac cggagcagtt
    57601 actgtgacaa agccattcgt agccggattc ggatacacca gcaaatcgtt ttctgctgaa
    57661 ggcaactcaa tatctgaagt aacgacatac ataggtggaa aaacaatgta atcgatccat
    57721 gcggcatcat caccatcata ataggagtaa tcttttatgt aagaccactt tagtgtcttt
    57781 gtgccggcgg taatccagaa tctttcctgc gaccaggcaa gctctccgct ccattcgccc
    57841 atggatgttc cgttgacaaa gaatttaaga aaatcccaac cctgctcgct cgaaaccttt
    57901 ttgtaaaaag acagggaatc atcggagaga acatcaatgg taattgataa atcggattgc
    57961 tggttgtcgg taatatttcc tgattgtgcg gcaaaagatc cttcatacgg agaaccggat
    58021 gtaataatcc aaggtgctcc accccctgtt acccattcgt attgtgtcag atcaccactt
    58081 tcccagtctt catctatctg cccgatgctt ctgttaaacg tctggctggc ctgatatacc
    58141 gacaaagcac tgagattgag tgtaacactg gcggccgttc ccataggagc cgatgatgaa
    58201 acttctacgg tgtaagtccc tgtttcggct ataccagtaa acaagctatc gtgctgccat
    58261 gctgctgagg ttatggtcag atatgggctg acagaactca ataatgccga agcatcccat
    58321 gcatgtgaat ggccattatt gacaacctga atattcatgg ctaccgtctc acccgcatcg
    58381 agcttattgt ttccattacc ccccacagca tcattgatgg tcagattaag aatctgtaac
    58441 aaaggcgcat tgattttaag attgaaatcc gatgaccagc tatgaccact gctgtctcga
    58501 atagaaatct ggaaaggagc catatactga tcgggaacca atgtgtcaat tttcactgta
    58561 aaagcattat tcaatgtcaa tgtatcgttt gaagctatca aaccccaaag cccgatgctg
    58621 tcaacaattt cgacataagg atcagaactt gaaaggatag catacactgt ggaatcattc
    58681 tgcaaaccat agtttttcaa tctgacattg agcccgacgg tttccatgat gtcggcttgc
    58741 tgattattat tggcagctgc atcatcaatg gatttgtttt ccatcagcac atacgggccg
    58801 cttggtgtct gaacaacaaa cgaagaaaca aaaggtttac ggaattgttt tgtcccaaca
    58861 atatcggcag ttccgggagt agtaaatggg atgatctcaa ttacaacatt gcctgtagca
    58921 tcggccattc cggcgccatg ccatactcca ttttgcgaaa caccaacata ggcgtagggc
    58981 tctgttgaaa cagtgatagt attctgtcca agcggaacca gagcaggatg tgtaacagaa
    59041 agctgagaag gcacactaag atacaccata agcgaagggt cgcccatcag atgataaatt
    59101 tcgctgtagt agtcaaacga agacggggag ccttcaagta cggcaaggtt tccggcaaga
    59161 atcatctgac cctgcgaaac aaaccattcg ctgaacggtt caccggtaat atgccatgta
    59221 cggtcataag ccccaagatt attggcatcg taaacaggat tggcattgat agttgtcctg
    59281 accccaacac cccaataaaa atcttcatcc caataagtac tgttcgaccc gccgatatat
    59341 cctatggccc ctttcatggg tgtacgcaac aatgcttctc caaaacattc cgattcatag
    59401 aaagtattgg tggaacaagc atttccaatc atcagcggat actggtgggc gttgttcatg
    59461 gatggaatgt ctgttttatc aaacgacgga tcgccccacc catcgctgta gccatgcgct
    59521 gtataattgg ccagagccac accattgcca atttccgttc tgatgattgc atcatcgctt
    59581 gcactggccg gatacagatg tgtccatgaa taaatgccat tggtggcatt gaaataataa
    59641 gttgtaccat aattgatttg cccgtttgca tgcgttggag cccatgaagc atcggcgcct
    59701 gcaatcatta caactgtatc aagccacgat tcggaaggaa aaagatattg ttcgtattca
    59761 agagttttat caatctgagg aagcaactga tcgacattgg ttgcgctgaa acgaccaaaa
    59821 taggcatcag caatcatatc gccagtaaat tcagcgtaat acatatcggt ataatgtgag
    59881 cccgctgttc cgctgaacgc cgggatctga gccacatcgc caacgatcag tatgaaagtc
    59941 ggagcaggat cggctgttgt tgcgttgtca tattttgcct gcaggtagtt gctgatgctt
    60001 gtggtagttg ttccaacagc cggatttgaa gtataggcct catcaacaat aaaacccttc
    60061 tttgttttcc atgcaataaa tggctgcaat gcatcatgaa acatggtgtc agcaacaata
    60121 atcattttta tgggataatg cgacgccgta tcgcgctgat gtatgaacgc ttcatgattg
    60181 attaagcgcg ctacaggtcc tgcaaaacaa acatcggcat atttttccga caactgattg
    60241 gttaaaactg catccccacc aggaaatgaa atattgagaa tcagtttgtc atacacacgc
    60301 agagtatttg aaaccggatt ataatcaaaa agatggatgt cgagcgaagc catccgaata
    60361 ttgcgcaaat taccatgaat atcaactgca accctttcat tctgacaaaa atcattgatt
    60421 ttatatgctg cattattgta ataaaaggga atttcacccg tatgactttt cgatacacct
    60481 ggctgatagg gcataagctg ataggcgttt ccggaaattg aagaaagatc gtaatcagta
    60541 taatggctgt aatcaatatc aacaagcacc tgagcatttt cgggcacctg aatgagccgg
    60601 ctgatggcgg gtaaaaaagg catgccggct tcggtccggg caaccattcc ctgaacaacc
    60661 atggaattaa acatgccggc ttcggtttta atcaccgcga tttcaaactg aggagccgta
    60721 acttcaatct gaagtccggc agaactcatg tttaaaactt ttacagcttc agcatctaaa
    60781 acacgggact gaccaaaacc cgcataagtc agcatgatgg ccagaatcag aacgagagag
    60841 cgtttcataa aatttgcttt gaaatgaaga caacaatact tgaaatatgt tgcaatagta
    60901 aataaaaata gttgattgtt gaaaagtttt tattgacaca actttgctaa tccgtatatt
    60961 tgcatcttta aaacggtaaa acttaacgat ttaacatgag attatcatta ctgacaattc
    61021 tcttttttgt ttcccttgca agtcaggcaa aaccgcaaaa taacaatgtg gatcgtctga
    61081 caataaatgg gtactatttc ggcaaaaatc tggtagtcat caatcccttg aatggagacc
    61141 gttttgccgt tgaatctgtt gaagtgaacg gcaaccccac gaccgacgaa atcaactcga
    61201 gtgtttttga aattgatttt gccgcaatgg cgctcaatca gggcgatccg gtcagtgtca
    61261 gcataaacta ttatgtcgga gccgaaaatc caattgtttt taatcccgaa gctctcgaag
    61321 ctgccagcaa tttttcattc aaaagctctt ttctcgaaaa gaaaaccgaa aaaatagcat
    61381 ggacaatcgc cggatcgcca ggcaccgaaa tgtttgaaat tgaacaatac cgatgggaaa
    61441 aatgggtacg tgttggtacc gtaaattcca aagacagcat ttcattaaat acctactcct
    61501 gcaaaattat tccacattac gggaaaaatc tgtttcgtgt caaactggtc gatcccaaag
    61561 gaaatattac atacagcaat cctgttaaat tgcagagcaa aacaccggaa gtgatgcttg
    61621 taaaaactac cgtcgaaaca accattgaat ttaccggcga aacattgtat cagatttatg
    61681 atgaaaaagg catacgcctg ctcaatggct ctggatctaa cgttgacgta tcttctctga
    61741 tggttggaaa atactgggtc aattatgaca ataaaaccga gcttattaaa aaaaaataaa
    61801 cagcaaaaac gctgtgagta atccaggccc ggctcctatt ccacttttta cagatcgtat
    61861 tcccgactcc gttttgcaga gtcaggtttt ggcacgcata accaatcctg cacttatcga
    61921 ccagggatac accaatcttt gcggaatggc ggtctgcgcc atgacattgg ccaaatacga
    61981 tgctatcggc tatcggaaaa tcatgacagg cctcaacgac agcaataagg ccgagtacaa
    62041 ccagtacaaa ctgaaagttc ctatccgcat tacaaaaaca gatgaaaact ttttcagaaa
    62101 aggaaaactg cccgaagctg attggttgct gctggcaact atgcgcaaca aatgcaatta
    62161 cattctgccg tatggcggcc gcatgtgcaa atggtacgaa aaaatcgcag gtagtaattt
    62221 cccttcggaa gtaagacgta cactcaaaaa aatgggcttc gaaaaaatca gcgacaacat
    62281 gaacggattc tggcccatac gcaaaccggc gctgcaaaca ctgcaaacac ttgattcagt
    62341 tttcactcag ggacacacac ccatcattct catcaataca cgaatgtatc acaaaggaac
    62401 tttttcggtg aaatcaaacc actttgtcat ctacaacgga aatctttcct ttgacaatga
    62461 aactgataaa gttactttca atgtctggac attcggatat ggtgcgggca agaccatcac
    62521 ctgctccatg gaagcattcc ggaataatta tttcggttgc attgtggtga aaaaaccggg
    62581 ataagcaata tatagggcct gctgaaaaag tctgaggtgc gccatattcg aggcgacaag
    62641 acg
//
LOCUS       DAOPKE010000007        62121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_484_length_62121_cov_11.697338, whole genome shotgun sequence.
ACCESSION   DAOPKE010000007 DAOPKE010000000
VERSION     DAOPKE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_62121_cov_11.697338"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..2507)
                     /locus_tag="PKY63_01840"
     CDS             complement(303..2507)
                     /locus_tag="PKY63_01840"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPE99383.1"
                     /translation="MLLTKEMLVFRNPVMSIVSSLLTNKTFRALFPPLLLLLIIVIVF
                     KEAAFFIHPLKWDALDCTMPWKYFISTCFADGVFPFWNPYQHVGYPFYGDAQSTLYYP
                     VTLVMTYFHGYTFKMLTVDYIFHVWVGATGMYFLGRSFKFSGIIAFILAVAYAFSGFM
                     IGNAQHIYWIISAAWLPFVLIFYIRMQHERRIWQALLLSLFLYLMISGGYPAFVIVLA
                     YIFAIVYFVKAIRLLKERKSNEFWNYTLLHGLVSVFTIGLSCLYLVTFFDNYSFSDRA
                     DGLMLSQVLQSPFSLESFISFILPLTTIKGFEHFGTDLSMANGYIGLFMFCFFILSLF
                     KRKNPVQKTILWISLVCLIISVGEKTYLREFLYHYVPLFDSFRFPSAFRLFVITGFIL
                     SAGFYLQSIEKNDNPAKLNKPLLLILIPAALFFVFSIFQTADISAGSMFQYIQSVKTG
                     DFSLSIGVLKFVQSILALVLLFLFISLLMVFKSMKNRLIIIAVFVVLDAGLTARMLAP
                     YTVYSPDLKISELNEFANQHFEKKYPQPDMTTDVIQHCDTISVRRYGLWRNLNNYYGQ
                     FAHGGFSSYVNRNIQLMEISFPEIYSAALRHRPVYVSECTKPYSLMGIADSISFCDYT
                     DAVQDSNVHDVNIAIVSYNPNRIEFNVNTPEPALVCYLQSWHKYWTATVNGKAREPVL
                     VNRFHLGVMSDAGNNTIVFEFRPKYFYTALIISLGTIMVLLVLLVFAGVRKI"
     gene            2620..3246
                     /locus_tag="PKY63_01845"
     CDS             2620..3246
                     /locus_tag="PKY63_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPE99384.1"
                     /translation="MPTRIRLQRFGKKAAPYYHVVVADSRAPRDGKFIENLGIYNPLT
                     IPATIDINFERALYWVKTGAAPSDTARAILSYKGVMMRHHLNVGVAKGAMTQEQADAK
                     FENWLSEKQSKIEQKASSVKQAGIDDKKNRLAAERKVKEDRDKAIAQKRQAEMKAEAK
                     EEAAVVEETPAVEEPVAETPAVETPVAEAPVADAPAAEAPETEEPKAE"
     gene            3262..3771
                     /gene="rimM"
                     /locus_tag="PKY63_01850"
     CDS             3262..3771
                     /gene="rimM"
                     /locus_tag="PKY63_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPE99385.1"
                     /translation="MEGLIRIGKTVKTHGINGEVLIKFEAGKTPANESEPVFLDFEGI
                     QVPFFIDSLRSPIDTEWFVIFEEVNDKTKAEKLLGRAVYVHQDNLALNEDEFSLDTLT
                     GYDVIDKKQGRVGVLSLIQKGAQDMMIIDNNGEEILIPFVEDFLVEINPDKKQIIVET
                     PDGLLDLNR"
     gene            3967..4623
                     /locus_tag="PKY63_01855"
     CDS             3967..4623
                     /locus_tag="PKY63_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPE99386.1"
                     /translation="MKVGTDGVTLGAYSSRNDFHYALDIGTGCGLIALMLAQKSTGKI
                     LAIDIDEPSVKQAASNFAASPWSSRLTARYVSAQDLALEVGGIFDRIVSNPPFFSDSL
                     KSSDTRRTNARHNTALSHRELAAAVNQLLSPDGFFDVILPIDVSISFESIMYYSGLFL
                     KEELILYSTADKPIRKIMSFARSYSGVFNSANLIIRQDAQHHTQEYIDFTADFFPGLK
                     "
     gene            4788..5417
                     /locus_tag="PKY63_01860"
     CDS             4788..5417
                     /locus_tag="PKY63_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99387.1"
                     /translation="MKLIAVIFVLSGLIASAQFDTLILKRNLAHYGSTPRYQVDTFAD
                     DGNMTYPPFLVGTTVLPGTRNQLVLKNYNLILDSVKFSPCNGEPPIVELNIDGLAGAE
                     SKESTFINSEFFKVQSLDLWKTGFTINIKIWENCCHHFLCDAVVIDDSILSLVYYPYG
                     WNYCACDCCFGLTYFFSFMGGQEEEFIKVKWVMINEWPETLLPLSFIPE"
     gene            5555..6154
                     /locus_tag="PKY63_01865"
     CDS             5555..6154
                     /locus_tag="PKY63_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE99388.1"
                     /translation="MKNKELQEQRMRDYFIQACKDLLKGEGLRSVSVRSVADRAGYSF
                     ATMYNYFRDINDLLFLCVNDFQNECNEFVAERVHKLPMGEARFRKAVQVYVEFFVEYP
                     GIFELFFMARKGDFGNRENILEVINNSLKTATIDEWNYLIKQGEISRSEVNNKLSLLQ
                     SWVTGVLIHYLNRLQPESYEEFQKTIKREIRQILPPGQD"
     gene            complement(6176..6748)
                     /gene="coaE"
                     /locus_tag="PKY63_01870"
     CDS             complement(6176..6748)
                     /gene="coaE"
                     /locus_tag="PKY63_01870"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPE99389.1"
                     /translation="MIKIGLTGNLGSGKSTVAKVFETFGFKILDADEMAKQLYHIPEI
                     RKQVELLLGVAIRNDDNTINYKIIADHYFNKPGIYAALNRILYPELQKNISDEIDSQQ
                     CNIIVEAAMLFEIEMQQIFDYVITVSTPVEERIQRVYKRNGSSREQFMEREKLQSQAE
                     WKETKADFVILNDERSSVIAQVSSILKNLK"
     gene            complement(6745..7629)
                     /locus_tag="PKY63_01875"
     CDS             complement(6745..7629)
                     /locus_tag="PKY63_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99390.1"
                     /translation="MNNKFRILIICISLAGLLWLVNHLNREYTVGVTFTVQYKDLVFP
                     ENNKPDSTIVAEVSGRGFSLIRFYFDRPYVIQLSKSDFKSLKQGDSLFLSVLPENLLK
                     STVDVLPAGISLKNIPNDSLSFSCISYPSKDVPVQVDVRLQENEHCAINGPVKIIPGF
                     VRITGPAEIIANIDSVKTQQILINNKCDTVRTTVKIAPFSNKKISCNISEIHVLIPLH
                     SVVNLQTKLHYNITILGHIYDGVIETEFMAPANEKEPELNLVLESEIVDKSLVFNVKS
                     PPGYKILKITPEFVPLKQ"
     gene            complement(7642..7974)
                     /gene="yajC"
                     /locus_tag="PKY63_01880"
     CDS             complement(7642..7974)
                     /gene="yajC"
                     /locus_tag="PKY63_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPE99391.1"
                     /translation="MNFAYILLMAPQSADGESSGGWSTMIMLLLLVVVFWLFFIRPQS
                     KKNKEINKFRQGLKKGDRVITIGGIHGKILEINDTTAIIETEGQGKLKLEKNAIAQEF
                     KAEEMAEK"
     gene            complement(7986..8441)
                     /locus_tag="PKY63_01885"
     CDS             complement(7986..8441)
                     /locus_tag="PKY63_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPE99392.1"
                     /translation="MKPGILIYSIFFTIMILAVSCSNSKNDKVNSDIVHNEATADGDE
                     TGNGPKISFEKTEHDFGDIMDGEKVEYAFKFTNTGNKDLVITSHSATCGCTVPEYPKG
                     AIAPGDGGVIKVAFNSKGKKGRNNKEIRISTNASERDVTLTIKANVIKP"
     assembly_gap    8801..8900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8948..10837)
                     /locus_tag="PKY63_01890"
     CDS             complement(8948..10837)
                     /locus_tag="PKY63_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPE99393.1"
                     /translation="MKKASFAEKLKYRFDSMMSRGPLAVIIWLAIATLLLVIIAATII
                     AAFGILPEDTEHMTFVEAFWQSLMRTLDAGTMGGDTGWGFRAVMLIVTIGGIFIVSAL
                     IGIISAGFDQKLEELRKGRSKVLESGHTLILGWSPKIFTILGELTIANQNQHRPHIVI
                     LSEVEKSEMEDQIKSLVPQTFNTKIITRSGDPLENADLEMVNYNKARSIIILPPEKEE
                     MHDLFIIKVALAIINNTGRKKEKFNIVAEIRDEKTLEVLDLIGRDEISVLQLSEVMAR
                     LTVQVAHQPGLSVVIEDLLRFDGDEIYFSHIPNLNGKQFRDIQLMFEDSTIIGVQKKS
                     GETKLNPPMNYILAAEDKIIAISEDDDTIAVNGHPEKAVREEKTTIVAEKIHKPVHTT
                     ILGYSPRMKMIIHELANYFPEGSTLNIYVQKNYKSKAEELKTEHLKINIHNGDITSRK
                     FLENLNLMNTDYVMILSEPDLSIQQADANTLVSLLLVRDIIQKNNYNTNIVSEMLDPQ
                     NSDLARISRKNDFIVSDKLVSLLISMISENKDLKPVIDDLLDADGSEIYFKPITDYVI
                     PGKNHNFYQISAAASDRGQVAFGYRIMTQSNNPEKAYGIVMNPKKSNTIVFNEEDMLI
                     VLAED"
     gene            complement(10845..12251)
                     /gene="rlmD"
                     /locus_tag="PKY63_01895"
     CDS             complement(10845..12251)
                     /gene="rlmD"
                     /locus_tag="PKY63_01895"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPE99394.1"
                     /translation="MASSSKVYGTVIENLEITDTASEGVCIGYHDGKVVFVPFTVPGD
                     IADVMLVRSKKKYSDGFLRTLHKSGESRVEPKCSHFTLCGGCRWQHMKYDAQLTMKQK
                     QVTDNLQRLGGLNIEKVYPISPSPEQFHYRNKLEFTYSDNRWLTDEERHAENAEPRGL
                     GFHLPGRFDKIFKVDRCYLQSDYMNTIRNTAQDMALQQNLTFCNRKEHSGFLRNLMIR
                     NSSAGHWMVTVIVGFDDQEMITNYLNALKTKLPEVQSWIYIVNTKANDSLNDLEFHVF
                     SGPAAFTETMEHLHFSVGPLSFYQVNVPQALAMYRMVRQLANLKGTETVYDLYTGTGT
                     IALFVAPHAAKVVGIEYVPTAIEDAKKNAVDNQITNAEFFCGDIAKTLNASFMAEHGH
                     PDVIITDPPRSGMHPDVVAQILNTAPEKVIYVSCNPATQARDIALMKEQYSVAEIHPF
                     DMFPHTHHVENIALLIRK"
     gene            complement(12254..13042)
                     /gene="cdaA"
                     /locus_tag="PKY63_01900"
     CDS             complement(12254..13042)
                     /gene="cdaA"
                     /locus_tag="PKY63_01900"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPE99395.1"
                     /translation="MLLFINELFHFRWVDALDILIVGYLLYEFYMLIRGTTAVYVGVG
                     IVLVYVLWKLVDLSGMHLLSEIFGAFISVGVLALVIVFQQEIRKFLTMLGSQNILTGR
                     SRRFLFWKVNDTSRNALDIEEVVEACFEMSQTKTGALIVLSLKNELKEIAATGAVFQA
                     QVQRELIKSIFYKNSPLHDGALIIRRNQIIAARCILPVSTRIDIPEDLGLRHRSAIGI
                     TEASDAIAIIVSEQTGHITYVEHGIMQRNIGKTELETLLKFNFA"
     gene            complement(13295..14008)
                     /locus_tag="PKY63_01905"
     CDS             complement(13295..14008)
                     /locus_tag="PKY63_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE99396.1"
                     /translation="MTRAIIIGATSGIGRGLASLCAANGYSVGVTGRRMQLLEELKAE
                     FPAIQIKQMDVTDCENAIIHLKELISEIGGLDLLVLSSGAGYMNNELDYEKEKVATDV
                     NVCGWTCLVDFVFNYFDQQKSGHLVAISSIAGLRGSRFAPAYGASKAYQINYLQALRQ
                     KASKLKHRITITDIRPGFVDTAMAQGAGIFWVAPVEKAARQIFTAIKRKRKVVYVTNR
                     WRLAAFIFRIIPRWLYERA"
     assembly_gap    14059..14158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14276..16003)
                     /locus_tag="PKY63_01910"
     CDS             complement(14276..16003)
                     /locus_tag="PKY63_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99397.1"
                     /translation="MRKILYGIAMFLFASMISTLKAQEKCDDCPRPRLIIYDIKPEVS
                     PEEAFGLNVEPGFFKQGLDDNDKSIGCITILDCHVVQDTSDSGDPVVFSTIVTAPEGA
                     INSHLNPNYIITSRIIKINNQKQIEFELQTAESREVVKRASMTVPSNYAPVNNNDAIG
                     YIIAKNNFVPLFNIIRNFEIRKRDQSVNIVIGSDHIIVEPEKQTVKAEEVIRVNLTFL
                     DCDKQPLKNRLIMLQGGTVCGTQVPGSTGGKFLNEFVKTNSEGRATAVFQADDKSSSA
                     QLLVYFDGMFPSGQSAGATGKAIIQINKKPTEKFTGNITATVTSKTSSNSDIAFTDQE
                     KTELTETFSCKMTFNSCIGCIEKLRNPEQRANILNNGFQYGIFSGSTAKNSAGEPQPI
                     QYSGHYKYWSTKYSSDDQMQIIMDKTTEGQTTGYDWFISAEIVNRKTSDANVVYLNNQ
                     YWVKSQIIDNIRMYSDNTKYKTTGKVWGKERDASGELKDFSETIDPQKSFGIPSGPDA
                     NEECNNADRKMLPLFIENPKEFEEYLLNPSGSYTLKLKGRRYSSEENETSDYQVLISI
                     VISPVYSED"
     gene            complement(16173..16811)
                     /locus_tag="PKY63_01915"
     CDS             complement(16173..16811)
                     /locus_tag="PKY63_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPE99398.1"
                     /translation="MLRKRLTRLILIFLLLMLGLVIFANVRISSHTRERVYSDVKTIP
                     YNKTGLLLGTSKYLKSGQINQFFKNRIVAAVELYKAMKIKKIVISGDNSRSDYNEPQD
                     MKDELMKRGVKAGDIYLDYAGFRTYDSVYRMSAIFGQQSFTIISQEFHNRRALYIASS
                     LGLDAVGYNAAEVSAYNSFKTKLREKFARVKVLLDFAFGKKPKFLGEKIQID"
     gene            complement(16853..17398)
                     /locus_tag="PKY63_01920"
     CDS             complement(16853..17398)
                     /locus_tag="PKY63_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99399.1"
                     /translation="MMTKIFINMGSYYMEVFSDVQEIKEYALKDNRLVSFTLHNSGCC
                     ADPQHLSYYYTAHYKNNIPSFIRIKTIGNISFTQKPDSLTEDSMDFSIVNDSAHLRMV
                     CYMLDETDCPPYHYSGTSIAKYGKCATGKLLGTRKEQGVDWVYVIMDVNPGKMNCYYP
                     SFTGQPTHIKGWMLKSDTDLK"
     gene            complement(17395..17850)
                     /locus_tag="PKY63_01925"
     CDS             complement(17395..17850)
                     /locus_tag="PKY63_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99400.1"
                     /translation="MYNEANEFCDMNAAFTHSLKQSNSDMIKYNRLFLFLFLLFAFLF
                     TSGQQPHTPLSQKEFEDSVACEKLFEWDDSIEYITDEFATNTPLSELETDRDSFQNMH
                     FIKSNKELQQILPYYSLPLESFVNTVFAIDLNGDSVKDYHTTALRAVHQ"
     gene            complement(17856..18692)
                     /gene="dinD"
                     /locus_tag="PKY63_01930"
     CDS             complement(17856..18692)
                     /gene="dinD"
                     /locus_tag="PKY63_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HPE99401.1"
                     /translation="MDNALIRQLNRLFEEAAYEQDGIEYWLARELQELLGYSDWRNFT
                     NAIEKAKQSCETSAQLVVDHFVDVNKMIELGKGGQREVSDIMLTRYACYLIAQNGDPK
                     KEQIAFAQSYFAIQTRKQELLEERIALNERLQAREKLVATETELSKNIYERGVDNKGF
                     ANIRSKGDEALFGGRNTLSMKRKLKIPENRPLADFLPTITIKAKDLATEITNFNVKKD
                     DLKGETNITKEHVKNNRDVRHLLGKSGIKPEDLPPEEDIKKLERRVKSADKDIAKKKL
                     KK"
     gene            18885..20177
                     /gene="tyrS"
                     /locus_tag="PKY63_01935"
     CDS             18885..20177
                     /gene="tyrS"
                     /locus_tag="PKY63_01935"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPE99402.1"
                     /translation="MKNFVEELKWRGMVHDMMPGVEDYLKEGMGTAYVGIDPTADSLH
                     IGHLVSVMMLKHFQLCGHKPIVLVGGATGMIGDPSGKSEERNLLDETTLRANQDGLKS
                     QLMRFLDFSESAPNAALMVNNYDWMKEHTFLSFIRDIGKHITVNYMMSKDSVKKRLSG
                     EAGEGMSFTEFSYQLVQGYDFLWLYQNRNCKLQMGGSDQWGNITTGTELIRRKASGTA
                     YALTCPLITKADGGKFGKTEKGNVWLDPEKTSPYAFYQFWLNTSDADAEKYIKIFTLI
                     GKEEIESLIAQHIQEPHLRLLQKRLAKEMTIFVHSEDDYNTAVEASEILFGKGTTETL
                     AKLPESVFLSVFDGVPMTEVPASSLQEGIPAIEFLSDVVGAFTSRGEARRMVKDNGVS
                     INKEKINEEFVVNSALLLNNRYILIQKGKKNFYLVKAV"
     gene            complement(20687..21232)
                     /locus_tag="PKY63_01940"
     CDS             complement(20687..21232)
                     /locus_tag="PKY63_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99403.1"
                     /translation="MKINYQLTAEDYLKFQLYTASRSKQVKARRMLTRIVWPLVFLAI
                     GIYFAVESVWVGAAIMAGVALIWFFTYPIYSRYFYKNHFRKEIKEKYTENFGKELSVE
                     ARSSDLLLTAPQGQRVIKYSSITEINDLGSHYLILFDTGTVAILPIGREVGRNVWDDF
                     IKQLALKSGKSVYDKKDWKWR"
     gene            complement(21229..22539)
                     /gene="hydA"
                     /locus_tag="PKY63_01945"
     CDS             complement(21229..22539)
                     /gene="hydA"
                     /locus_tag="PKY63_01945"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HPE99404.1"
                     /translation="MDTGLIKNCTVVNPDGTFDADVYVNEDQIIELIGKPGTLDVNSP
                     KIFEGTGRYLIPAGIDPHVHLKLPTPAGPSCDDFGLGSFAAMMGGTGAIIDFVTPARG
                     QSLIEALDAREHESVACAIPLKFHMGITWWNNAVAEEIRQCIEEYDITSFKVYLAYRN
                     TIGLDFTQLKEVMKCLSYYGAVLAIHAEEGDVIQELQNKFIIEGKLEPKYHAYSRPPE
                     TEYNAVEKVIDLVRETGCKTYFVHMSTAESVRLIRKAKKEGLPVYAETCPQYLLLDKE
                     VYEQFYYMVFPYIISPPIRGAADREELWKGLADGTIDCVSTDHCPFTLRQKMEGRFDF
                     TKIPNGAGGLHYRMQLLFTHGVLQNKITMQQFVQLTSTNAARIFGFEDYGYIKAGIPS
                     NLILWNPDHKDTISFKDSLTDADISIYDNFEVFGNAEKILIHYA"
     gene            complement(22615..22965)
                     /locus_tag="PKY63_01950"
     CDS             complement(22615..22965)
                     /locus_tag="PKY63_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPE99405.1"
                     /translation="MKKSKESYRYRLQHIMDAISKIELISKDLTRDEFISDITASSAV
                     CYQFLIIGEAIASLPVEITDKYKYPWYKPRSFRNFLAHEYFGVDLIMVFDTINKDLPE
                     LGQLIDRMISDMQP"
     gene            complement(22952..23533)
                     /locus_tag="PKY63_01955"
     CDS             complement(22952..23533)
                     /locus_tag="PKY63_01955"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE99406.1"
                     /translation="MESFCTYIRNQRTSRGMTLRQVAADTDADAAVLSKVERGIRLAS
                     RNLVIKLAVYYSLDEAALLKMWMQSKWSRELTESRTLVNEPVSVYAQTVMPTFAEIKQ
                     KVSAILRKDKRILKAFLFGSFSRNEATDFSDIDILIKTDNRFSFTLFDLAEIAHKTEK
                     VLGRKTDVVTERALSPKIQESIHDNLKVLYEKK"
     gene            complement(23614..24870)
                     /locus_tag="PKY63_01960"
     CDS             complement(23614..24870)
                     /locus_tag="PKY63_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE99407.1"
                     /translation="MILLKNATYIHPETFEITTGHMLVDEAPGAGFQFVPADTKADKT
                     IDCHGLLVTQAFINAHHHVYSALSRGMNAPKKSPTNFTETLEYIWWTLDQCLDQDMVR
                     LSALTTAMNCAKHGCTFAIDHHASPNFLKGSLETIAKAFDEVGVGHLLCYEITDRYGI
                     EKTRESLEESDEYLNSRRGLVGLHASFTVEEDTLLKSVAIADKHKTGIHIHVAEDPMD
                     EKFTKEKYGCSVVQRLERAGALSLKNNIFGHGLHMDVKERTLVRESGTWMAVNFDSNL
                     NNRVGFFTSTELGDRIMLGTDGMHSDMIKSAQTALFAGQNFDNVSWIDIYTRLRNGAR
                     YLRESNIPHGSNNLVILDYDSPTPVTQNNFLGHFYFGLEGRHVKHTIADGKLIYSEGN
                     ILTADEDSILKESRKAAQMLWDKMSE"
     gene            complement(24922..26121)
                     /locus_tag="PKY63_01965"
     CDS             complement(24922..26121)
                     /locus_tag="PKY63_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99408.1"
                     /translation="METLRETSRQYIADTDMTFIRYALDKVRWDQRLTGITGARGAGK
                     STLMLQYLKANFSDSDKALYVSCDDLYFGNNTIVSLANAFVKQGGTHLFLDEVHKYPG
                     WSREIKNIYDRYKKLTVIFSGSSALNIYKSGADLSRRALQFHLNSMSLREYIELFYGI
                     QAPVISLEDILLRSTKTAEKLMKTVDFPIRYLHEFYQTGSYPFARDDTKGYHRRLLST
                     INQVVETDLPALFNVDYTAVVAIRKLLAIMAGLVPFKPNITELGREMGLSRETIIRYI
                     QYLSRAEIFASLYAETKGLGLLSKPEKVYLQNPNLLFALADIRIPEKGTIRETFFMNQ
                     ISALHTVNISPVSDFLVDGKYTFEIGGKSKKKTQIAGIRNAFVVQDDIEYGHTNVVPL
                     WLFGFLY"
     gene            complement(26355..28904)
                     /gene="xdh"
                     /locus_tag="PKY63_01970"
     CDS             complement(26355..28904)
                     /gene="xdh"
                     /locus_tag="PKY63_01970"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HPE99409.1"
                     /translation="MIKFVLNNKPVEFGGNAEMSLLNWLRNEQGITSVKDGCNGQSAC
                     GACLVEIDGKAKLSCVTKMGSLEGASIQTLEGIPPKVRDLIAKAFVEKGAVQCGFCTP
                     GFVMRTKILLQENPDPTIDEIRTALKWHLCRCTGYKKIEKAIEYSAELIAENKSCEND
                     NHHKGVGDFMPKYEAFETAIGQRKFVNDLSFPGMLHGALRFSDHPRAKVLKIDTSEAL
                     KSVGVEQVFTAADIPGNRIVGLIYKDWPMMIAVGETTKYIGDVLAGVVADTAENAREA
                     AKKIIIEYDVLTPVSDAHEALKDSSPQVHDTHANLLENCILKQGNADEVIAKSKYVYH
                     NNFETQRIEHAFLETESALAMPDGDGVLLYSSGQGIYEDRRQVASVLGLSEEKVRVIL
                     VPNGGGFGGKEDLTVQGHVSLFAFLLKKPVKLTLTREESMIMHPKRHPVWMDITLACD
                     ENGKLSAMKFSAVGDTGAYASVGTKVMERIAGHATGGYFVPVIDLQAKTVYTNNVPCG
                     AMRGFGANQAAFALESCVDELCKQGGFDCWQFRWDNALIDGLTTATGDHVEGVGIRAC
                     LEAVKPYYDKARFSGLATGIKNSGVGNGMVDYSDVIIEIKSPEKVVLHHGWTEMGQGV
                     HTVAAQILCEETGIDSSVIEVIVDTAAGIKTGMTTSSRGTVLLGNAILEAAKTMRADL
                     QGKTLADIVGHRYQARWECTWTVKPGTICENSKTHYGYGYAAQLVILDDHGKIEKIVA
                     AHDAGKIINPVMFEGQIEGAVHMGLGYAISEDYPYKNGYPDYSKLRECGVLRAHETPE
                     IEVIGVEVPDPVGPYGAKGLGEIGLVPTAGAVGNALCHFDGIRRTQLPMRK"
     gene            complement(28971..>29668)
                     /locus_tag="PKY63_01975"
     CDS             complement(28971..>29668)
                     /locus_tag="PKY63_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE99410.1"
                     /translation="AQIVPVAGTYDDAFDLSVALTEKTGIFNRNTAYNPFTIEGKKTV
                     SFEIFTQYGNKAPDKVFVPVGDGVIISGVYKGFEDLLKLELIDKMPVIVAVQAEGSNN
                     IVRNLYSDSFVSLPSKTVADSISVDIPRNYFMARRFLKKYQGETIEVSDSEILSASKS
                     LSSNTGVFAEPAAATAFAGLLKQITEEKISANENVCVLLTGSGLKDVNAVQPVINIPE
                     AVNPQEFLNDFRI"
     assembly_gap    29669..29768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29769..30080)
                     /locus_tag="PKY63_01980"
     CDS             complement(<29769..30080)
                     /locus_tag="PKY63_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99411.1"
                     /translation="MQRKYFFKCTDCGAEFHDVSFMYLCPFCAAKNTQGQPPKGVLKT
                     IYDFDELKNQSHKQDSFFTSQGWLPLLPINETKSLGHLSVGNTPLIPVSELNEQKLPF
                     KL"
     gene            complement(30085..31554)
                     /locus_tag="PKY63_01985"
     CDS             complement(30085..31554)
                     /locus_tag="PKY63_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE99412.1"
                     /translation="MIEILDHVVNADIRKKAAKRFKEKGIILPTFAQMRNPELVPAEV
                     KEKLKTIGLWDLDPSNLFRITWKNEPVKTGGGFGKVNYIEIPKEITGVKARILMLVGK
                     YFPTGAHKVGAAYGCLAPRIISGQFDPEFHKAVWPSTGNYCRGGAFDSWLMGCTAVAI
                     LPQEMSKERFEWLHEIGAEVYATPGCESNVKEIYDKCWEIRRTKPDHVIFNQFDEFGN
                     PVWHYSITGPAIEEIFGCEKNERSRLTAYVSNTGSAGTIAAGDYLKKLYPNIKVVASE
                     ALQCPTLIANGFGGHRIEGIGDKHVPWVHNVKNTDVVTAIDDEDCMRAIRLFNEPEGR
                     KFLKSIGVQDDVINRLDLLGISSVANLISAIKTAKYYEMNEDDVIITVATDSMEMYDS
                     RIKELNEERGAYTNLQAGRDFEKTMLAASIDYLKELTYSDKKAIHNLKYFTWIEQQEK
                     PLEDLNQLWYDHDIWNHLFAQTAKWDEMIEEFNQEVKKA"
     gene            complement(31707..32744)
                     /locus_tag="PKY63_01990"
     CDS             complement(31707..32744)
                     /locus_tag="PKY63_01990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99413.1"
                     /translation="MTDILFCGKDTFSYHRTKVILSGLGKLDNVHVNLLKISSRDKNH
                     ALKLKQESSQHDFVFVPAFRHKDVAWIKKHSKAPVVFDPLISEYLTKVVDYGQWYKAP
                     TKYLLDFKAIHAADYLIADTEAMKKYYSRFYHFPETRIAVISVGFIPDDFKASIQEKQ
                     DRLFHVGFYGSFVPLQGTNVIAKTALLLRNENIVFDIIGNGATYKDFLSDIKKYNLKN
                     IHLHGWLPYEKLPAAISNFDIALGIFGHSGKAKRVIPNKLFHYAVMNKCIITRKSDAI
                     NEIFTPGTNIVSIEANAGKLAESIMELKNNPSVKSKIAENGYRLITQNYNDKEITLKL
                     LNFLKPLTYTI"
     gene            complement(32765..34705)
                     /locus_tag="PKY63_01995"
     CDS             complement(32765..34705)
                     /locus_tag="PKY63_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPE99414.1"
                     /translation="MRVTPGLKILVLVIVVAAAALRLFNLFHIPFSSEELNVILTAKN
                     ISFSQLLNNVIPNDSNPAGIQVLLFFYIKLTGLNEGWIKLPFILAGIASVYLMWKIGR
                     EWYSPAVGLLSASTLAFMHFFIIHSQMALPYAGGLFFVLLMLLGWARLVLLTTNNYRY
                     NFLLFITGAILSSYIHYFSLIIVVSLILTGLFYLRGKDLKKYLIACIIIIIAIVPHIP
                     LTLNQLKITEFNHVSVTSATDYSIGFLKYAFDFSWIVAGAFVLIALYGFFAAALLRKI
                     RFGKFHVVLLVLTTISVASVFLFSNNYPFFNRYSLMLFSFPLIIVFVFSFYEKINLRH
                     VIGITVIWSLILIHSLIWSRDHYQYFYHSPYEETGREIKAFTQTHSLDSIMVLCSYDQ
                     KIADFYKDKYGVANKLHYFFYRSNKDFSDLLKQLSDEQYHYLIIAENAQTPMSLYALS
                     HEYFSVSLQESHYNQGSCIVMKRGEPHYRNYLRGSLCNFSKHSNVCWEFDTCHVLTDT
                     LKAAKILKCGNNNQHCISWTQHISGYLRSQANIFDATIWIYLPDTLEKEAFWVTELVS
                     DTSIITVNKTSVNTDLLPTNTWVPVSSGLFFPDIRKIPFDAKIRVYLCNKNTTNIYVR
                     QAFAGIRAGNPSIYWLTDGLFD"
     gene            complement(34702..35838)
                     /locus_tag="PKY63_02000"
     CDS             complement(34702..35838)
                     /locus_tag="PKY63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPE99415.1"
                     /translation="MEKFNDIRPYRNEEFKAAFNQLIQEEEFISVLKYFFHEKYTLHL
                     EKLQDVKTIDEFQVLLIKPILQGIINSTITDLSFTGAENLSAGQPCLFISNHRDIVMD
                     PSLIDLGLHFNNIPTVEIAIGDNLLIKPWIETFVKINKSFIVRRSLSGRDLLNSSVLL
                     SEYIRHSIVDRSSYLWIAQREGRAKDGNDRTQESLLRMLGLSGNSKDLRKALTPLNIS
                     PVSISYEYDPCDGLKALELRTKAIGKPFVKTPQDDLKNMITGIGGKKGKVNFHFSEPI
                     NELIQQSPETDNKKQSAAIAKMIDNVIFSNYTLYDTHKLAYNLLSETNTFEIAESSAF
                     RIKNYFIQQLNNVGLSENELHFILIQYANPVINHLGIKQKNTTK"
     gene            35944..38733
                     /locus_tag="PKY63_02005"
     CDS             35944..38733
                     /locus_tag="PKY63_02005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPE99416.1"
                     /translation="MRCLFIVLLLFVFIPFNQIFAQDADKFRMPAGCSSNDYIPGHVI
                     VKFSSDVSDRAGNRDFLALMQELNVVRFYQKFPHSKMPSEKVNEWGMPMVDISGIYEL
                     CFDSSVSVEFLINRLLSAGLFEYACPHYIEHQLYVPDDPNIGSQYYLTNIRAYQAWDI
                     CKSDTNRIVAITDTGYEFNHPDLSAAVKHNYDDPIDGVDNDNDGYTDNFSGWDMGSWD
                     NNPQYAASGHGIHVSGLAGASADNGFGMAGVGFKSRLLPVKVDNANGNLVATYEGVVY
                     AADHGATVINCSWGSTYSGNLYGQDIVNYASYNCNALVVAACGNANNMNMYYPASYNN
                     VLSVAGTDANDYKSSNSSFGYKVDICAPGIGIYSTWSGASFVTSGGTSMASPIVAGAA
                     ALVSSWYPQYNMLQIGEILRNSADIIDTISANQSYDGLLGMGRLNMHRALTDSITPAV
                     RLVEWAFSDSADMEFDPGDTLFIGAKALNFLDTSSSLLYARLECMTPEVELIDSIWMI
                     GTLNTLQYANNHFAPFKVKLSNVPPSTNIAFLVHFYDSTWHSADYISTNVNKDYNTLD
                     TNQLTLTVTSHGNFAYNNLVNLSQGEGFRFNDGSNLVSGFGFMCGTDMFHLADNLYSI
                     VFPVDSDFVADNFVLDLNPAPMGDQCLFSQYTDQGADSASQMNIIVRQWSYAWNTPED
                     RNYILVRYQVVNDSIVGLDDFYTGLFADWDIINSAENRCWYDPAIDAIVATTTDSVMF
                     TATALLSPYSSKHYAVDMDGFNSSIALTDGFSESEKYSMMKTNRLYAGTDLAGNDIAA
                     MVSSGPHTIAPFDTLELAYVIVAGTNFTDIADALARARIRYLDPDLGNASDDALPFLI
                     YPNPALDRLHYFIPAGNDRYDVYISDMTGKLCIEKHDVEKEGQFDVSILPAGLYTVSF
                     VSGKTCSVMNFTIVR"
     gene            38730..39560
                     /locus_tag="PKY63_02010"
     CDS             38730..39560
                     /locus_tag="PKY63_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE99417.1"
                     /translation="MTMSLDNNAKSWNILIPYYFDAFSDYALQEGIALSKVFGAGLVL
                     FHCNPDNTVKIRLAEMANNIISNHNIRTECFIPEGNQADLLFALAHKTESILIIIGHN
                     CQKTSLGFSLKKTIRIMRKSRTPFLMVPEGLHEKSFRDIVYCMGYQKTEKEKILWASY
                     FGRICKSRIHVAMPKVSDQFFKTGIRSNFQAMNKLYNAVEIDFKMVAMESNIHHAVSA
                     AIEYGHSIQAGAFIILTTLRPDIFDFFGGADDIAAIRNKYSLPVFCINPRDDLYVLCN
                     "
     gene            39693..40286
                     /locus_tag="PKY63_02015"
     CDS             39693..40286
                     /locus_tag="PKY63_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99418.1"
                     /translation="MKLKAIIQIILALGILVLIYFIYAGIMSPVKFNNEMTSRKDIVI
                     QKMKDIRTIQSMYMTLNGKYCSTFDSLITFVEEGEMPVVKLTARPGDSTFTDPIRDTI
                     DFISIKDSLFGKRPDFKLENIAHIPFSNFENAPYDNFEMKVAVVKKGNVPVNVIEVFA
                     ANKYYLKGLDLEKNNIDPEDGLRFGSLTDPSTDGNWE"
     gene            40286..41086
                     /locus_tag="PKY63_02020"
     CDS             40286..41086
                     /locus_tag="PKY63_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPE99419.1"
                     /translation="MNIHSEYCFLPQGTVLPDESIKVLGVHSEGFYYLIHRDNEILYA
                     LLGSFRNGITASSLMAVTGRKPEFRMLEIDHLLLMPHSFVAVPDEFLATQNESQLFEM
                     VQVLPESFRLIKSELSDTGASLLSAFSSGDLQVLQQLMPKYPLKDMIASWFSKIKPYD
                     AANEVHLFILPSSFAIVVFAEGKMQLINTFQYSDRNDFLYYVLGAVQSCHVRPDATSL
                     MLCGEINPSSPLAEALGTYFPQIRYDAVENNGDADSRRLSSMLYPLFQ"
     gene            41086..41616
                     /gene="rsmD"
                     /locus_tag="PKY63_02025"
     CDS             41086..41616
                     /gene="rsmD"
                     /locus_tag="PKY63_02025"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPE99420.1"
                     /translation="MRIISGSIKGRTFTPPERNGVRPTTDMAKEGLFNILQHRVALDG
                     ITVIDLFSGTGSISYEFVSRGAEMVFSVEKDSVMIDFIQSIAKKLNLSNIKPIRSDAY
                     RFLALTGVKADIIFADPPYGTPGITDIPELVFSKKLLNDDGMLIVEHDSHLNFTSHPH
                     FIEERKYGKVHFSIFQ"
     gene            complement(41610..41945)
                     /locus_tag="PKY63_02030"
     CDS             complement(41610..41945)
                     /locus_tag="PKY63_02030"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPE99421.1"
                     /translation="MKNFFFVIAFVSLIINASAQKIFSADSRYDPDIKIFVVDSRYDA
                     DLLVYKCDSRYDASGNKGLWYFVDSRYDADKKIFFVDSRYDADLLIYFIDSRYDAGWR
                     NTAKQHLLY"
     gene            complement(41994..43142)
                     /locus_tag="PKY63_02035"
     CDS             complement(41994..43142)
                     /locus_tag="PKY63_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE99422.1"
                     /translation="MSIYLDNSATSWPKPAGVISSITAYISEYGGSPGRSGHHFATKA
                     AHEVLQTRELIASLFNCPAAERVMFSANATHALNFAIKGILNQGDHVIISHMEHNAVF
                     RTVSFLQSEGLISFDIAHCNEQGFLDTKHLKSLIRKNTRMIICIHGSNAAGTIQNIAD
                     IGAICNEHDILFLVDAAQSAGFVPIDMVEDHIDLLAFTGHKKLYGPTGIGGLCLKDGI
                     AIRTLIHGGTGSKSDLAEHPMVYPDRLEAGTINAVGVIGLKAGIEYILDKGINNIRKT
                     LTDLTNYFIAECTQIDNVSIYGPTISENRLPLISMNIKNMKPTDLAAILDKEYGIMVR
                     AGLHCSPLAHQTIGTFPNGTLRFSFGCFSTDDEVKYAIESLKKIVSQK"
     gene            43390..45183
                     /locus_tag="PKY63_02040"
     CDS             43390..45183
                     /locus_tag="PKY63_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPE99423.1"
                     /translation="MKEKITSIIESFSNDAARLMDILSEVQSEFGCVSCEAVSVIASA
                     LQISEADVEQTRSFYHFYTKDPVGKYAIYLNNAVVACMMGMGEIVDEFEKQLGIKMGQ
                     RTADNMASLHFTACIGMSDQEPAAIINDVVFTSLTRDKVKAIVADMKAGKCVKDMVKE
                     VGDGNNANPLVHSMVKNNIKKAGPVVLDAYESGAGLAKALGTTSQAVIGEVQASGLRG
                     RGGAGFPTGMKWMFCAKESGDKYVLCNADEGEPGTFKDRVLLTERAAMVFEGMAIGGY
                     AIGAKEGILYIRREYKYLQALLEDTLKNMREKNLLGKNIMGKGFDFDIRVQIGAGAYV
                     CGEESALIESAEGKRGEPRNRPPFPAQKGYKNKPSIVNNVETFCAVSRILDKGGAWHK
                     GFGTDESAGVKVLSISGDCKNPGVYEIEWGMTIQQMLDMVGATDVQAVQVAGPSGVCL
                     NPEQFGRKIALEDLATGGSMIVIGKNRDLLKDVVLNFMEFFADESCGSCVTCRSFNMI
                     LRNAIKKVVEGNAIKKDVEDMLAWSEIIRKTTRCGLGQTSANPISTTINNFRHLYDAR
                     VKDVEFASLFDMSKAVAESCEYVNRKPVVHE"
     gene            45217..45921
                     /locus_tag="PKY63_02045"
     CDS             45217..45921
                     /locus_tag="PKY63_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPE99424.1"
                     /translation="MSETVKFTIDGKECTGQKGQYIVDAAADNGVYIPVLCHMRDVVP
                     AGSCRVCTVKVNGRNMAACTTPVEDGMVVENVTEELTDIRKAIVEMLFVEGNHFCPSC
                     EKSGNCDLQALGYKYQMMVPRFPYAFERRNIEATAPKIYLERNRCIFCKRCIRMIKDK
                     DGKSIFAFKGRGQHLEITMDLKLAANMTDEMAQKAMDICPVGCILKKEVGFIDPIGQR
                     KYDKNVIGSDIEKITK"
     gene            45934..46482
                     /locus_tag="PKY63_02050"
     CDS             45934..46482
                     /locus_tag="PKY63_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99425.1"
                     /translation="MSKPIVATTSLAGCFGCHMSLLDIDERILKLIELVEFNKSPIDD
                     IKTFTKQCDIGIIEGGCCNSENVHVLKEFRKHCKILISLGECAIMGGLPALRNGIPVK
                     ECLEEAYLNGPTVGLNAEKILPNDDELPMILDKVYPCHEIVKIDYFLPGCPPRADLIW
                     EAVAALVTGNPMNLPYEVVKFD"
     gene            46502..47953
                     /locus_tag="PKY63_02055"
     CDS             46502..47953
                     /locus_tag="PKY63_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPE99426.1"
                     /translation="MAQKVTIEPVTRVEGHGKVTIQLDDNMNVTQSRLHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKALDVIVGAGTGEGLTPTGEKMRRLMHY
                     GQMLQSHALHFFHLSSPDFLLGYDADPAIRNVIGVALNFPDLAVQGVMMRKYGQEVIE
                     RTAGKKIHGTGAIPGGINKCLSAEEKAFLLNGSDMMNIDKMISWADAAVQFFKEYYMK
                     NKAMIDGFAYFPSNHLSLVRKDGALDFYHGVIRAVDADGNRVLDDVDYQEYNKYIEEE
                     VRSWSYMKFPYMKHLGKEKGWYRVGPLARLNTCDFIPSPLAQKEFEFFKSQTNGKPNN
                     YCMHTHWARLIELLHAAEVIKDLLNDPDIMKNDLVTKGTKQSEGVGLIEAPRGTLFHH
                     YRINEHDQITMANLIVSTTNNNEPMNQSVNFVARRMMTGQKEITEPMKNQVEMAIRAY
                     DPCLSCATHALGQMPLDIKLIDSKGEILDRYKS"
     gene            48070..48528
                     /locus_tag="PKY63_02060"
     CDS             48070..48528
                     /locus_tag="PKY63_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPE99427.1"
                     /translation="MKKTLIYGYGNPGRMDDGIGKRFVDLIDNWIAEEKIENIFTDCN
                     YQLNIEDASIISEYDAVVFVDASIVEDVENFRLEKVEPNDATIEFTMHAVSTAFVVDL
                     CRKMYNKIPEAWVLHVRAYEFDFKEEMTPKAVENMLDAFVFLKDFLMKMA"
     gene            48782..50200
                     /locus_tag="PKY63_02065"
     CDS             48782..50200
                     /locus_tag="PKY63_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99428.1"
                     /translation="MRTIVGRDFEKDELESFYQSDVSEFVAVYGRRRVGKTFLIREFF
                     RDRFDFYLTGLANGGFSEQMANFNLALREAGLKDLPLAKNWIYAFQQLRMLIEKSRKK
                     KKVIFLDEMPWLDTPKSKFMSALEHFWNSWASGRNDILLIVCGSSTTWMTDKLLNNKG
                     GLHNRITHQLRLEPFTLKECAAYVKSLKFTWSQHQVAECYMALGGIPYYWSLLKKGKS
                     VDQNIDHLFFGANARLKDEYKNLYEALFKNSDKYMQVVEALSKKSSGLTRQEIVESIE
                     ESSGGGLTKILEDLENCGFIRSFQAYNKKSRDRIFQLCDFYTLFYFRFVRRAGRDKKY
                     WKSMMDTTQHRAWAGYAFEQLCTMHVDQLKHALGISGVYCNVYSWRSPAGTDKGAQID
                     LIIDRNDNVINLCEMKFSAHPFVINKKYADEIKNKSGTFRSVTNTKKSVFLTFVSSSG
                     LAQNVHSGIVQCEIELKDLFAV"
     gene            complement(50293..51003)
                     /locus_tag="PKY63_02070"
     CDS             complement(50293..51003)
                     /locus_tag="PKY63_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99429.1"
                     /translation="MTAETSVLLTTTASIAIVHTLAGPDHYLPFIAISKARNWSLKRT
                     VVFTFLCGLGHVGSSVLLGIIGVATGIAISHIELWEGQRGSIVSVLLMAFGLGYFIWG
                     TIRAIRNKPHTHLHLHENGEQHEHIHSLDSTHGHAHTKKKRNITPWILFTIFIFGPCE
                     PLILLVMYPAAESNYLLLITVTALFAIITIGTMITLVVLANRGFHFLNLKKAGRYAHA
                     IAGFIILLCGIGMVFLGL"
     gene            complement(51014..52135)
                     /locus_tag="PKY63_02075"
     CDS             complement(51014..52135)
                     /locus_tag="PKY63_02075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HPE99430.1"
                     /translation="MKIQSFTPIGIVHNLIDNSEDYHKIKNQPSTIEIFDEYLEALTG
                     IEENEFLDIVFYFHKSESTLISNTKNRPDISRGVFGNRSPRRPNLIGVTSVRLIERNE
                     NMLLVKGLDALNGTPVLDIKNCDTSLFAYEADNNPLHQAKLKSSPRIDIRNEIYSNRY
                     ESLMIKAAQMHGHFCPGLAMGIMAAAKAMNLLEAESDGMEDLLAITETNNCFSDGVQF
                     VTGCTFGNNALIFKDLGKTAFTLTHRDGKGIRISSKADSQQVIRDAFPDFQNLYQQVV
                     ANQNHDSLLVSEYRKSSLNRAFGTLSIPFESLFNVQHVQTDIPQYAKIHDSIICATCG
                     ESIMSTRTADNTENKAVCLTCGGFEYNMLDGNGIHFKIK"
     gene            complement(52144..52911)
                     /locus_tag="PKY63_02080"
     CDS             complement(52144..52911)
                     /locus_tag="PKY63_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE99431.1"
                     /translation="MSISISNISFSYSKNQVLKNLSIDIRQGEFIVLAGPNGSGKSTL
                     IKCINGILQPQSGQIKISNANIRLYPKNKLAEKLAYVPQNKSVLPNLSVFDTIMLGRK
                     PYINWRPSTKDLAITSQIIEQLQLESISMSSINELSGGQQQIVFIGRALAQQPDIILL
                     DEPTAHLDIKHTVEIMKLLKTLSAKGLTIIVALHDINTAIRFADRILMLRDGEVFASG
                     GNEIITEKNLEKLYDVKVRILQEENSFHVIPLEPIEK"
     gene            complement(52914..53984)
                     /locus_tag="PKY63_02085"
     CDS             complement(52914..53984)
                     /locus_tag="PKY63_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE99432.1"
                     /translation="MKNSSVINEYRKNSRKKLLVIASICILYVATIIIALLSGSADYQ
                     LNDVFQLITGNSDAFTEQILFQIRIPRILGAMLAGTALAVSGAAIQSLLRNPLASPFT
                     LGISGAAAFGAALCIVFFNSTALPAGISESAIHPYLISLSAFICSLAGVVLILALSRL
                     KGATPEIIILAGIIISSLFGAGISAMQYFADTVQLSSIVFWTFGDLSRAHYDNLLILS
                     FIVIPATIYFILQSWNYKALQTGDEYAQSIGVRVKKVRMRGMIIASLVTAVVVSFYGI
                     IAFVGLVVPHIIRRITGDNEQYLIPVSALFGGLFLLASDTLARSIFSPVVMPVGILTS
                     FLGAPLFLFVLIKGMKKPVNYS"
     gene            complement(53984..55084)
                     /locus_tag="PKY63_02090"
     CDS             complement(53984..55084)
                     /locus_tag="PKY63_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HPE99433.1"
                     /translation="MMHYRLLHSSVLLIIVFSFICCNNPGRAEQSDPDIVVTDMLGRK
                     VTVPANIDKVVALRAGALRLLVYMDAADMIAGVEENEKRSSRPYLLAFPELMKLPSVG
                     PQMGGDPELIVAAQPDVIFISYSTVGEADALQQKTGIPVVAIDCPDLQVNSNGLFDSF
                     SLIGKILHKENRADSLIKGISSMIEELNTRTSSIKPEDKPGVYVGGVAYSGAHGIAST
                     HGAFAPFLFVNANNVVSGKQNIVSSHVKGTNIDIEQLLLWNPDIIFIDESGYSLALQD
                     INNQPALKKSLDAIRSNQLYCLLPYNNYATNYEFVLLNAWFAGKIIYPQQFEDIDIET
                     KASEVLTLFFNKPVSLSQMKSALGLRKITPVH"
     gene            complement(55091..57121)
                     /locus_tag="PKY63_02095"
     CDS             complement(55091..57121)
                     /locus_tag="PKY63_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99434.1"
                     /translation="MTGRLTFFIPIFILMLTSTALAQTNEISDTISIKEMIITATKTE
                     RNPLEIPARINIIDSVLLNQTAALQTDDLLRFIPGVNVNRNTGLYTQRPMVTLRGLSG
                     DEQGRTLVLVNNVPVNTSDEGGVNWNRINQNDIERIEIFKGPGSSLYGNNAMGGVINI
                     ITKKAEKPSEAICSAGYGTYNTFRQDLNVRIRNPKGYYAVLSQFYQQSDGYNSVPDTN
                     RTQYDIARSLEAMGISARTGFDNSRWLNWELQYDVFRDQRGEGYQIHTPEGCYRNFNT
                     NLLRGMLRGGNKNTSYTFNTYYQLEHYYDVNEKLNGTGVYTRYDVNSKRTDMGMLANV
                     NRILNQNNTLTAGFEYKSGGIQGGDCYQTAPYDTIYNEGNINTLACYLQDEQSFLKKK
                     IRLLAGIRFDQVSFVNGLHENTNPWNTIPELRDNTWRKVSPRAGLRFNFIKQLSAFVS
                     YSHGFRASILDDLIRTGWMWVGPKYANPELKPETMENFEAGFDIFPAKNLKIALSAYY
                     MQGNDFLYYVATSDSIYGRPIYRRENVTSVEIKGFEAEATYTLTKNILFSGSYTFSDS
                     KIVGFTERPDLENKSLKYVPAHNASFSAFWQNKIINVSGRALYKSAQFADDENNITLD
                     SYLTFDLMISRKINENLSLSLDIQDIMDNQQMEVNGYLSPGRLINGRITLKL"
     gene            complement(57246..57647)
                     /gene="nikR"
                     /locus_tag="PKY63_02100"
     CDS             complement(57246..57647)
                     /gene="nikR"
                     /locus_tag="PKY63_02100"
                     /inference="COORDINATES: protein motif:HMM:NF002169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPE99435.1"
                     /translation="MKVKRFGVSLDEQILSDLDHLVNKHKFPNRSQAIRYLISNYLVE
                     EKTDGNKIVAGSIILVYDHHKRELQNKSTAIQHDYHDIILSVMHVHIDHHNCLETIAV
                     MGKSGKLKELADQLIAIKGVKHGKLVITTTE"
     gene            58382..59716
                     /locus_tag="PKY63_02105"
     CDS             58382..59716
                     /locus_tag="PKY63_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99436.1"
                     /translation="MFDRKAIQNMNRWMNKKNRKPLVLRGARQVGKTTLVKVFAKQFG
                     TFLHLNLEDKRAAELFSVSSGMEDLLAAIHLYCNIPAGKGKTLLFIDEIQNSPEAVAR
                     LRFFYEEMPQIYVIAAGSLLESMLNKSIRFPVGRVEFLAIRPCSFSEFLNATGEKMLA
                     ESIDKMSMPVALHEKASHLFNRYTLVGGMPEIIAEYSESKDIVSLSGLFDSLLTSYKD
                     DVVKYARNETMEHVIGLILQEGWKYAAQTISMTGFAGSAYKAREMGEAFRTLEKTMLL
                     ELCYPTSGAILPIQPETKRQPKLLWLDTGIVNYVAGIQSEVFGSPDILDVWRGRVAEQ
                     VAAQELLTLDDRVTYRRSFWMRDKKGSDAEVDFIVQNGSSIIPVEVKSAHNAHLKSLH
                     SFMDIAPHNIAVRIWSKPFDVSMVKTGKGKEFRLISLPFYYISKLHEWIDRE"
     gene            complement(59770..60675)
                     /gene="hypB"
                     /locus_tag="PKY63_02110"
     CDS             complement(59770..60675)
                     /gene="hypB"
                     /locus_tag="PKY63_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPE99437.1"
                     /translation="MCDTCGCSDPNQKVTYHKPGEEHSHEHAHVHPHEHQYAHAHNHE
                     HDHEHHDHEHEHNYIHSHSHDGHAHSHDHTHEHAGHDHDHAHPHGKEFVLAHNILDKN
                     QSLAERNRGYFDAKNINALNLVSSPGSGKTTLLEKTISALKNDLNFYVIEGDQQTMND
                     ADRINATGAPVIQINTGNGCHLDSEMISKAVKELNVADNSMLIIENVGNLVCPSLFDL
                     GEKARVVIISVTEGDDKPLKYPNMFSTSNICIINKTDLLPYVDFSVEKVKENALKINH
                     HLQFFEVSARTGEGMDKWYEWLKKA"
     gene            complement(60687..62009)
                     /locus_tag="PKY63_02115"
     CDS             complement(60687..62009)
                     /locus_tag="PKY63_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPE99438.1"
                     /translation="MEISHNWRQVTGLMVARNFVEVDANVLYPRIDDFNGGSDIIAME
                     FPSMNYLHYLFSELFGYTHWYGRLINLVVSSLGLFFFYKCAKTAGISERTAFYATIIL
                     SASIWFSFSRKMMPDTYCISLMFIALYCGLKFLHNHKYPLLFLYVVFSSLALLSKIPA
                     GIYLVVLLPFVLSRKFELKNKITISVVTAVPLILAYWWYFIWNPYLASEYGNWYNIGK
                     PMLKGIKELSDNLGKTLDNFYFDAFSGFIMFGVFAAGLFLMIYKKNTPLFIGFTLLFL
                     VFLIYIFKSGFFFYHHNYYIIPFVPVMALLAGYAIASINKRWIMFSVLLLGITESIAN
                     QQHDFFIKDSEKYKLTIESILDKFSSQKDLILVNGDGNPQLMYLAHRKGFNCNNYQLN
                     DSTYIANVKNNHCRFAVVNKHSRPDTYYLQSSYNTVFDNDDFRIFALN"
BASE COUNT        17165 a        12581 c        13517 g        18558 t
ORIGIN      
        1 tgatggagaa gattggcctg ccgattacgt aaaagtgaga aaagttacgg attaagtcgg
       61 aaacattagt aattttccat ttcaattcgg tagcccgtaa gggcttatat attaatagca
      121 ataaaatata tcgaattctt gcccgtaggg catgaacatg cgaaatttcc tgcaaaatat
      181 acaatgttaa tccccttaat ctggccctgc tgaaatttaa acacaccata aaacggaaga
      241 aggataaaaa gtaaggataa agtgtgattg ttttcgatta taaatcacga aaaaaactgg
      301 cactaaattt ttcttacacc tgcaaaaacc agtaacacca gcaacaccat tatggttccg
      361 agacttatta ttaatgcggt atagaagtac tttggtctga actcaaaaac aattgtgttg
      421 tttcctgcat cactcatgac tcccaaatga aatctgttca ccaaaaccgg ttcccttgct
      481 tttccattca ctgttgcggt ccagtattta tgccatgatt gcagataaca aaccaatgcc
      541 ggttccggtg tatttacatt gaattcaatt ctgtttggat tatacgaaac gattgcaatg
      601 ttgacatcat gtacgtttga atcctgtact gcatctgtgt aatcacaaaa tgaaatgcta
      661 tctgcaatac ccattaaaga ataaggtttc gtgcattctg aaacatatac tggtctgtgt
      721 cgcaacgcag cagaatatat ctcgggaaaa gaaatctcca taagttgaat gtttcggttt
      781 acataagaac tgaaaccacc atgagcaaat tgtccgtaat aattgtttag attccgccat
      841 aatccatatc gtcttacact gatagtatcg caatgctgta ttacgtcagt agtcatgtct
      901 ggctgaggat actttttttc aaagtgttga ttggcaaatt cattcagttc actgattttt
      961 aaatcaggac tatacacagt ataaggtgcc agcattctgg ctgtcagacc tgcatccagt
     1021 acaacaaaca ctgcaattat gatcaggcgg tttttcattg atttaaaaac catcaataaa
     1081 ctaatgaaca aaaatagcag cacaagtgcg agaatggatt gaacaaattt aagcactccg
     1141 atgcttaatg aaaagtcgcc ggtttttaca ctttgtatgt attgaaacat cgacccggcg
     1201 ctgatatcag cagtttgaaa aatcgaaaaa acaaaaaaca atgcagcagg aatcaatatc
     1261 agcagcagag gtttattcaa ttttgctgga ttgtcattct tttctatgct ttgcagataa
     1321 aaacctgcag acaaaataaa acctgtaata acaaacagcc ggaatgccga aggaaagcga
     1381 aaagaatcga acagtggcac atagtgataa agaaattctc ttaaataagt tttttcaccg
     1441 acggaaataa taagacaaac aagtgaaatc cacagaatgg ttttttgaac aggatttttc
     1501 cgtttgaaaa gcgacagtat aaaaaagcaa aacatgaaca gacctatata tccattggcc
     1561 atcgacaggt ctgtgccaaa atgttcaaat cctttgatgg ttgttaatgg cagtatgaac
     1621 gaaatgaagg attccaacga aaacgggctt tgcaaaacct gtgaaagcat gagcccgtcg
     1681 gctctgtctg aaaaactgta attgtcaaaa aaggtaacga gataaagaca cgaaagtcct
     1741 atggtgaaaa ctgaaactaa accatgcaat agtgtgtaat tccagaattc attgcttttt
     1801 ctttccttca gtaaacggat ggccttgaca aaataaacaa tagcaaaaat ataggccagt
     1861 acaatgacaa atgcgggata accgccactg atcatcagat atagaaacaa tgaaaggagc
     1921 agtgcctgcc atatcctgcg ttcatgctgc atccggatat aaaaaatcag gacaaaagga
     1981 agccatgccg ctgaaataat ccagtaaata tgttgtgcat tgccgatcat gaaaccgctg
     2041 aaagcataag ccactgcgag aataaaagca atgatcccgc tgaatttgaa tgaacggcca
     2101 agaaaataca tgcctgttgc tccgacccag acatgaaaaa tgtagtcaac cgtcagcatt
     2161 ttgaaggtat atccgtgaaa ataggtcatg acaagggtta ccggataata caatgtgctc
     2221 tgggcatcac cgtagaaggg atatccaaca tgctgatatg gattccagaa aggaaaaaca
     2281 ccatcggcaa aacaggtgct gatgaaatac ttccagggca tggtgcaatc cagcgcatcc
     2341 catttcagcg gatgaatgaa aaatgctgcc tctttaaaga caatgacaat aatcaacagc
     2401 aatagcagcg gcgggaaaag cgccctgaat gttttattgg taagaagcga cgatacaata
     2461 ctcataaccg gattcctgaa aaccaacatt tcttttgtga gtaacaaaga taacagactt
     2521 ttttttgtgc aggatttgca acttcagcga attctttcca tttatttggt caattcaaaa
     2581 aaaaatcgga catttgcacc cctaattaaa caaacacata tgccaacaag aatcagactg
     2641 caaagatttg gtaaaaaggc cgctccttat taccatgtcg tcgttgctga cagtcgtgca
     2701 ccacgggatg gtaagtttat tgaaaatctt ggcatttaca atccgctgac aatcccggct
     2761 accatcgaca tcaacttcga acgtgccctt tattgggtaa aaaccggggc tgctcccagc
     2821 gacactgccc gcgctatcct ttcctacaaa ggcgtcatga tgcgtcatca cctcaatgta
     2881 ggtgttgcca aaggtgccat gactcaggaa caggccgatg cgaaattcga aaactggctc
     2941 agcgaaaaac agagcaaaat cgaacagaaa gcaagcagtg ttaaacaggc tggtattgac
     3001 gataagaaaa atcgtcttgc cgctgaacgt aaagtaaaag aagaccgcga caaagcaatt
     3061 gctcagaagc gtcaggccga gatgaaagcc gaagctaagg aagaagctgc tgtcgtggaa
     3121 gaaactcctg cggttgaaga acctgtagcc gaaactcctg ctgttgaaac tcctgtagct
     3181 gaagcaccgg tagctgatgc tcctgcagct gaagctccag agactgaaga acctaaagcc
     3241 gagtaattcc ggtatccacc catggaagga cttatccgta tcggaaaaac tgtcaaaacg
     3301 catgggatta atggtgaagt tctgataaaa tttgaagccg gcaaaactcc ggcaaatgaa
     3361 agtgaacctg tatttctcga ttttgaagga atacaggttc ctttttttat tgattcgctt
     3421 cgcagcccga tcgacacaga gtggtttgtg atattcgaag aggtcaatga taaaaccaag
     3481 gccgaaaaac tgctgggacg cgcagtatat gttcatcagg acaacctggc cttaaacgaa
     3541 gatgagtttt cgcttgacac gcttaccggc tacgacgtca ttgataaaaa acaaggtcgc
     3601 gtcggtgtgt tatcgcttat tcagaaaggc gctcaggata tgatgatcat cgacaacaac
     3661 ggcgaagaaa tattaatccc tttcgtagaa gattttcttg ttgaaattaa tcccgacaaa
     3721 aagcaaatta ttgtcgaaac acccgatggg ctgctggatt tgaaccgata gttttaaagt
     3781 tatcaaaggg ctaaaaccct tcggtagctt agttccattc attattattg caattaaagg
     3841 taacttctgc tattatcaaa gatgtcttat ctttgctttg cacaattact ttttctgcgt
     3901 caatccgcat caacaatgga tcctttccgg tttaaacaat tcgctgttta cgatcatcac
     3961 tcgactatga aagtcgggac agatggcgta acgctgggtg cctatagcag ccggaacgat
     4021 ttccattatg cgcttgacat cggcaccgga tgcggactta ttgcacttat gctggcgcag
     4081 aaatcgaccg ggaaaattct ggccatcgat attgacgagc catccgtaaa acaggccgcg
     4141 tcaaatttcg ctgcttcgcc ctggagcagc cggctgacag cccgctatgt atcggcacag
     4201 gatcttgcgc ttgaagtcgg tggaattttc gaccgcattg tgtcaaatcc accctttttc
     4261 agtgatagtc tgaaatcttc tgatacccgc cggaccaatg cccgtcacaa cactgctttg
     4321 tcgcaccggg agctggctgc agccgtcaat cagcttctgt cgcccgatgg tttttttgat
     4381 gtcattcttc cgattgacgt atcaatatct ttcgaaagca tcatgtacta ctccggcttg
     4441 tttctgaagg aagaacttat tctgtattcg acagccgaca agccgatccg caaaatcatg
     4501 tcgtttgcgc gcagctacag tggtgttttt aattcagcga atctgattat acgtcaggat
     4561 gcgcagcatc acacgcagga atatattgat tttactgccg attttttccc gggactgaaa
     4621 taggagagtt tgccacgatg gcacgaagac acttattctc ctttcctgtc gcggattttc
     4681 aatccgtgac aggacatcga cttagacgaa tgcgctacca gaaactgaaa tagaaataca
     4741 atccctgcaa atattttcta tttttgaatt attaatattt atcgttcatg aaactcattg
     4801 cagtcatttt tgttttgtct ggcctgattg cttcagctca gtttgacacg ttgattctga
     4861 aaaggaatct ggctcattat ggctccactc cacgttatca ggttgacact tttgccgatg
     4921 acggcaatat gacttatcca ccttttttag ttggaacaac ggtattacct ggtactagaa
     4981 atcagttggt attgaaaaat tacaatttaa tccttgatag cgtcaaattc agtccatgca
     5041 atggtgagcc gccaattgtt gaattaaata ttgatggttt ggctggagct gaatcaaaag
     5101 agagcacttt catcaattcg gaatttttca aggtacagtc tttagactta tggaaaactg
     5161 gttttacaat caatattaaa atatgggaaa attgctgtca tcatttttta tgtgatgcag
     5221 ttgtgattga tgattcaatc ctttcattgg tatattatcc ttatggttgg aattactgtg
     5281 cttgcgattg ttgctttggg ctcacctatt tctttagttt catgggcggc caggaagaag
     5341 aatttattaa agttaagtgg gtaatgatta atgaatggcc cgaaacctta ttaccgcttt
     5401 cttttattcc tgaataatta attatacaga tagattcgct acagagaaca gtgaaatact
     5461 attacaattg tgtcattatg attgttgaaa aattaattaa tgttcaatat atgaacattg
     5521 ttcactaaat ttgcagtgtt caaaatatgt aattatgaaa aacaaggaac tccaggagca
     5581 gcgcatgcgc gattatttta ttcaggcctg caaggatctg ctcaaaggcg aaggcctgcg
     5641 tagtgtgagt gttcgcagtg tggccgatag ggcagggtat tcctttgcca ccatgtataa
     5701 ttatttccgc gacatcaacg atttgctttt tctatgtgtc aacgattttc agaacgaatg
     5761 caatgagttt gttgcggagc gggtgcacaa actgcccatg ggtgaagccc gcttcagaaa
     5821 agccgtgcaa gtgtatgttg agttttttgt tgagtatcca ggcatttttg aattgttctt
     5881 tatggcccgc aaaggcgatt tcggcaatcg ggaaaatatt ctcgaagtca tcaacaattc
     5941 gctgaaaacc gccaccatag atgaatggaa ttacctgatc aaacaaggag agatttcgcg
     6001 cagcgaagta aacaacaagc tttcattgct acagtcctgg gtcacgggcg ttttgattca
     6061 ttatctcaat cgtctgcaac ctgagtcgta tgaggaattt cagaaaacca tcaaacgcga
     6121 aataaggcag attctaccgc cggggcagga ttgatttttt gatgaaaaat tgaaattatt
     6181 tcaggttttt cagtatgctg ctcacctgtg caattaccga tgatcgctca tcgttgagga
     6241 ttacaaaatc agcttttgtt tctttccatt cggcctgtga ctgcagcttt tcgcgttcca
     6301 tgaactgctc ccggctgctc ccgttgcgtt tgtacacacg ctgtatgcgt tcttcaacgg
     6361 gcgtggaaac tgtgatgacg taatcaaaaa tctgttgcat ttctatctca aaaagcattg
     6421 cggcttcaac aataatattg cattgttgtg aatcaatttc gtctgaaata tttttctgca
     6481 gttccggata caggattcta ttcagtgcag cataaatgcc tggtttattg aaataatggt
     6541 cagcaattat tttgtaattg atcgtattat catcgtttct gattgctact cctaaaagta
     6601 gttctacctg ctttcggatt tccggaatgt gataaagttg tttggccatt tcatctgcat
     6661 caagaatttt aaaaccaaaa gtttcaaaaa cctttgctac agtcgatttt ccactgccca
     6721 gatttccggt aaggccaatt tttatcattg tttcaaaggg acgaattcag gcgtaatctt
     6781 cagaatttta tagccgggtg gtgatttaac gttgaaaaca agacttttgt caactatctc
     6841 gctttcaagc accagattca gttccggctc cttttcgttg gctggtgcca taaactcagt
     6901 ttcaataact ccatcataaa tatgaccaag gatcgttata ttgtaatgaa gttttgtttg
     6961 cagatttaca acagaatgca ggggaataag aacgtgaatt tcggaaatat tacaactgat
     7021 tttcttgttt gaaaacggag caatttttac tgtagtacgc actgtatcgc atttgttgtt
     7081 tatcagaatc tgttgtgttt ttacagagtc gatatttgca attatttcgg caggtcctgt
     7141 tattcttaca aaaccgggaa ttattttaac cggtccgtta attgcacagt gttcattttc
     7201 ctgcaatcga acatctacct gtaccggaac atctttcgag gggtaggaaa tacaggaaaa
     7261 tgaaagggaa tcgtttggaa tatttttaag actgattccg gcaggaagaa catcaaccgt
     7321 tgatttcagt aaattctcag gcaatacact taaaaaaagc gagtcgccct gttttagtga
     7381 tttgaaatcc gattttgaca gttggataac atagggtcga tcgaagtaga atcggataag
     7441 agaaaaccct ctgccagaga cttcggcaac tatagttgaa tccggcttgt tgttttcagg
     7501 aaaaacaagg tctttgtatt ggacagtaaa agttacacca acagtgtatt cacggttcag
     7561 gtggtttaca agccatagca ggcctgcaag tgaaatgcag ataattaaaa tcctgaactt
     7621 gttgttcatt gtgaacagac attatttctc agccatttct tcagctttga attcctgggc
     7681 gattgcgttt ttctccagtt tgagcttgcc ttgtccttct gtttcaatga ttgcagttgt
     7741 gtcgtttatt tcaaggatct ttccatgaat accaccaata gtaatcacgc ggtcaccttt
     7801 tttcagacct tgacggaatt tattgatttc tttgttcttt ttcgactgcg gtctgatgaa
     7861 gaacagccag aaaacaacaa caagaagaag aagcattatc atggtggacc atccaccgct
     7921 tgattcaccg tcggctgatt gtggagccat gagcaaaatg taagcaaaat tcatatttgt
     7981 tttttttaag gttttattac gttggctttt attgtcagag tgacatctcg ttccgaagca
     8041 ttggtgctaa ttcggatttc tttgttgttg cgcccttttt tacctttact gttaaaagcc
     8101 actttaatca cgcctccgtc gcccggggct atggcgcctt ttgggtattc aggcacggtg
     8161 cagccacagg tagccgaatg tgaagtaatc acaaggtctt tgtttccggt attggtgaat
     8221 ttaaaggcat attcaacttt ttctccatcc attatatcgc caaaatcatg ttcggttttt
     8281 tcaaatgaaa tcttcggtcc attgccggtt tcatcaccat ctgctgtggc ttcgttgtga
     8341 acaatatccg aattcacttt gtcatttttt gaattcgagc aggatactgc caatatcatg
     8401 atcgtgaaaa aaatgctgta aatcagaatg cctggtttca tctctgtttt tctttttgga
     8461 atacaaaaat acaaaaaatc cttcaatttt aattgaataa attccttcac ggcaccttta
     8521 tctgcgcgca agcttcatat ctgcagcgtt tttcgttaac gtatgaacat acatcatcat
     8581 tgagcttcac gtaaaccgtg acacggtcgc agcttttttt gaacccggga ttttgtccag
     8641 ggctaatatc tgccgcgccg atagcgcttt ttgaatttca cgtgatggca agtcgtagct
     8701 tattatacgg cttctccacc aaaacctaaa ctacttaacc ttaatcttaa ccttaacctt
     8761 aaccttaacc ttaaccttaa cctcgccgct tcctagtctt nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn cttaacctta accttaacct taaccttaac cttaacctcg
     8941 ccgcttccta gtcttccgcc aacacaatca gcatatcctc ttcattgaaa acaatggtgt
     9001 ttgatttttt gggattcatt actatgccat acgccttttc gggattgttt gactgagtca
     9061 ttatccgata accgaaagca acctgtccac gatcggatgc tgcagctgaa atctgataaa
     9121 agttgtggtt tttccctggt ataacataat ctgtgatggg cttgaaataa atttctgatc
     9181 cgtcggcatc gagtaaatca tctataactg gctttaaatc tttgttttca ctgatcattg
     9241 atatgagaag acttacaagt ttatcactta caataaagtc gttttttcta ctgatacgtg
     9301 ccagatcact attttgagga tcaagcattt cgcttacaat gtttgtattg tagttgtttt
     9361 tctggatgat atccctaaca agcagtaatg ataccagtgt gtttgcatct gcttgttgta
     9421 tgcttagatc tggttctgat aaaatcatca cataatcagt attcatcaga ttcagatttt
     9481 cgagaaattt tcgtgaggtg atgtcaccgt tgtggatgtt gattttcaga tgttctgttt
     9541 tcaattcttc cgctttgctt ttgtagtttt tctgaacata aatattcagg gtcgatcctt
     9601 caggaaaata attggcaagt tcatgaataa tcattttcat gcggggactg tatcccagaa
     9661 tagttgtatg aaccggtttg tggatttttt ctgcaacaat tgttgtcttt tcttctctta
     9721 ccgccttttc cggatgaccg ttaactgcaa ttgtatcgtc atcttccgaa atggcaataa
     9781 tcttgtcttc ggctgccagt atatagttca tcggaggatt cagttttgtt tctccgcttt
     9841 ttttctgaac tcctataatc gtgctgtctt caaacattaa ttgtatgtcg cgaaattgtt
     9901 ttccgttcag atttggaata tgtgaaaaat aaatttcgtc accatcaaat cgcagcaaat
     9961 cttctatgac aacactgagc ccgggttgat gagcaacctg aactgtcagt cgggccatca
    10021 cttctgataa ttgcagaacg ctgatctcat cccgacctat cagatcaaga acttcgagtg
    10081 ttttttcatc acggatttcg gcaacaatgt tgaatttttc ttttttcctt cctgtgttat
    10141 tgataattgc cagtgctact ttgatgatga acaaatcatg catctcttct ttttcaggcg
    10201 gtaaaatgat aattgaccgg gctttgttgt aattgaccat ttcgaggtct gcgttctcta
    10261 atggatcgcc cgaccgtgta attatttttg tgttgaatgt ttgaggaact aaacttttaa
    10321 tctgatcctc catttcagat ttttccactt cagataatat cacaatatgt ggccgatgct
    10381 gattctgatt ggcaatagtc aactcaccca atatagtgaa aatttttggt gaccatccaa
    10441 gaataagagt gtgtcccgac tccagaactt ttgaccttcc tttacgcaac tcttcaagct
    10501 tttgatcaaa ccctgccgat atgataccaa tcaaagccga tacaatgaaa attccaccaa
    10561 tggtaacaat aagcatgacc gcacgaaagc cccatcctgt gtctccgccc atggttccgg
    10621 catccagtgt tctcatcagt gattgccaga atgcttcgac aaatgtcatg tgttcggtgt
    10681 cttctggtaa tattccgaat gcagctatga tggtggcagc aataataaca agcagaagcg
    10741 ttgcaatagc cagccatatt attactgcaa gcggtccacg cgacatcatt gaatcaaagc
    10801 ggtatttcaa cttttcggca aaggatgctt ttttcatatg tactttattt tctaatcagc
    10861 aaagcaatat tctcaacatg atgtgtatgc ggaaacatgt cgaatggatg aatttctgca
    10921 acgctgtatt gttctttcat cagtgctatg tcgcgggctt gtgtggcggg attgcagctg
    10981 acgtaaatta ctttttcggg agctgtattt aaaatttgcg ccaccacatc gggatgcatc
    11041 ccggaacggg gcggatcggt aatgataaca tccggatgcc cgtgttcggc catgaatgag
    11101 gcgttcagcg tttttgcaat atcaccacag aaaaactcgg cattggttat ctgattgtcc
    11161 actgcatttt ttttcgcatc ttcgatggct gtcggcacat attcaatgcc tactactttg
    11221 gctgcatgag gcgcaacaaa caaagcaatg gtgccggttc cggtgtataa atcatatact
    11281 gtttcagttc ctttcagatt agccaactgg cgtaccatgc ggtacatggc caatgcctgt
    11341 ggtacattca cctgataaaa cgacagcggc ccaacgctga aatgcagatg ctccatggtt
    11401 tcggtaaaag cggcaggtcc cgaaaacaca tgaaattcca gatcattcag cgaatcgttg
    11461 gctttggtgt ttactatata aatccacgat tgtacctcag gtagtttggt tttcaatgca
    11521 ttcagataat ttgtaatcat ttcctgatca tcaaaaccaa caattacggt taccatccaa
    11581 tgcccggctg agctgtttcg gatcatcaga ttgcgcagaa atccgctgtg ttcttttctg
    11641 ttgcaaaatg tcagattctg ctgcaaagcc atatcctgag ctgtgtttcg gatagtgttc
    11701 atgtaatcgc tctgcagata gcatcgatcg actttgaaaa ttttatcgaa gcgtccgggt
    11761 aaatgaaatc ccagtccgcg aggttcggca ttttctgcat ggcgctcttc atcagtaagc
    11821 cagcgattgt cggaataggt gaattccaat ttgtttctgt aatggaattg ttcaggtgac
    11881 ggagaaatgg gatatacctt ttcaatattg agaccgccca ggcgctgcag attatcggtt
    11941 acctgtttct gtttcatagt cagctgcgca tcgtatttca tatgttgcca tcgacaaccg
    12001 ccgcaaagtg tgaagtgtga acacttaggt tcaacgcggc tttcgccgga tttatgcagt
    12061 gtacgcagaa atccatcaga atattttttt ttgcttctaa ccagcatcac atcagcaata
    12121 tcgcctggta cggtgaacgg aacaaagaca acctttccgt cgtgatatcc gatgcaaacg
    12181 ccttccgatg cggtatcggt gatttcaaga ttttcaatta cggtaccata tacttttgag
    12241 gaagaagcca taatcaggca aaattaaatt tcaacaatgt ttcaagttcg gtctttccga
    12301 tgtttctctg cataatgcca tgttcaacat aggtaatatg ccccgtttgt tctgaaacga
    12361 taatggcaat ggcgtcgctc gcttcggtga ttccaatggc tgatcggtgc cgtagtccaa
    12421 ggtcttcggg aatatcgatc cgggttgata ccggcaaaat acatcgggca gcaataatct
    12481 gattgcgtct gataatcagt gcgccatcgt gcagtggtga gtttttgtaa aatatcgatt
    12541 tgataagttc ccgttgaacc tgtgcctgaa aaacagcacc ggtagccgca atttctttta
    12601 attcattttt taaggaaaga acaatcagtg cgccggtttt tgtttgactc atctcaaaac
    12661 aggcctccac cacttcttct atgtccagcg catttcggct ggtatcattt actttccaga
    12721 aaagaaaacg ccggctgcgt cctgttaata tgttttgtga acccagcatt gtaaggaatt
    12781 ttctgatttc ctgctgaaac acgattacca atgcaagaac ccccacactg atgaatgctc
    12841 cgaaaatttc actcagcaaa tgcatgccac tcagatccac cagtttccat aatacgtaca
    12901 ccaatacaat gcctacgcca acgtaaacgg ccgtggttcc gcgaatcagc atgtagaact
    12961 catacagcaa atacccgaca atgagtatgt cgagggcatc gacccatctg aaatgaaaaa
    13021 gctcgttgat aaaaaggagc atcggtttgt tttttgcaaa aatacgaatt taaggcatag
    13081 ggcacgcagc atgggaaatt cgaatttaga gcatttattt gcaaaacaag tatatcctgc
    13141 actgggtgca cgtctccagc ttgtaaccac gtaaaacgag acaaggtcgt cacttgacct
    13201 cgaccttaac actcttgtaa tgtctgtgac tttgtaacga tttccgcttc cagcggaata
    13261 aattccacgt gtaacgtctg taacgacagt aaattcaagc ccgctcgtag agccacctcg
    13321 gaataatgcg aaaaataaaa gctgccagtc tccacctgtt tgtcacataa accacttttc
    13381 gcttgcgctt gattgcagtg aaaatctgac gtgctgcttt ttcaacgggg gctacccaga
    13441 agattccggc tccttgagcc atggcggtat cgacaaatcc aggcctgatg tctgtaattg
    13501 taatccggtg tttcaatttc gaggcttttt gccgaagcgc ttgcaaatag tttatctgat
    13561 atgctttgga tgctccatac gcaggtgcaa agcggcttcc gcgcaatccg gcaatggagc
    13621 ttatggcaac cagatgcccc gacttctgtt gatcgaaata gttgaaaacg aagtccacaa
    13681 ggcatgtcca gccacacaca ttgacatcgg ttgccacttt ttctttttca taatccagtt
    13741 cgttattcat atatccggct cctgaactta gcaccaacaa gtcaagtcct ccaatttcag
    13801 aaatgagctc tttcaaatga atgattgcat tttcgcaatc agtgacatcc atttgtttta
    13861 tttgaatggc cggaaattcc gccttcaatt cttcaagcaa ttgcatccgc ctaccggtta
    13921 ctcccacgct gtatccattc gcagcacaca gcgaagccag tccccgtcct attcccgatg
    13981 tagcgcctat gatgattgct ctggtcatcc cgacaatttt ttgtcatgca aaagtacaat
    14041 attgaaatgt gttttatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
    14161 catcccgaca attttttgtc atgcaaaagt acaatattga aatgtgtttt atgaacaaaa
    14221 aagaggaacc aatttgattc ctctttcttt aataaatgtg aaggtagatt tggctttaat
    14281 cttcggaata gacaggtgaa attacaatac tgatgagcac ctgatagtcc gatgtctcat
    14341 tttcctctga tgaatatctt cgtcctttaa gcttcaaggt gtagctgccg cttggattca
    14401 aaagatattc ttcaaattct tttgggtttt caatgaataa gggcagcatt ttcctgtctg
    14461 cattattgca ttcttcgttt gcatctggcc ctgagggaat tccgaaactt ttttgtggat
    14521 caattgtttc cgagaagtct ttaagttcgc cggatgcatc gcgttcttta ccccagactt
    14581 ttccggttgt tttatacttt gtgttatcgc tgtacattct tatgttgtca attatttgtg
    14641 attttaccca gtattgatta ttcagataaa cgacattagc atcgcttgtt ttccgattga
    14701 caatttcagc actgatgaac cagtcgtagc cggttgtttg accttcagtg gttttatcca
    14761 tgataatctg catctgatca tcactgctgt attttgtgct ccagtattta taatggcctg
    14821 agtactgaat gggttgtggt tcaccggcgc tgtttttcgc cgtggatcct gaaaaaattc
    14881 catattggaa tccattgtta agaatgtttg ctctttgttc agggtttctt aatttttcaa
    14941 tacacccgat gcatgagttg aaagtcattt tgcatgaaaa ggtttctgtt aattcagttt
    15001 tttcctgatc ggtaaatgca atgtcagaat tggaactggt ttttgatgtg acagttgctg
    15061 ttatatttcc cgtgaatttc tcagtaggtt ttttattgat ttgaatgatt gcttttcccg
    15121 ttgcgccggc gctttgtccg cttgggaaca ttccatcaaa atacacaagc aattgtgctg
    15181 aactgctttt gtcatcggcc tgaaaaacgg ctgttgctct gccttcgctg ttggttttaa
    15241 caaattcgtt tagaaatttt ccaccggtgc ttccgggtac ctgcgtaccg caaacggttc
    15301 caccctgcag catgatcaag cgatttttca atggctgttt gtcgcaatca agaaatgtca
    15361 gattcacccg gattacttct tctgctttta cggtttgctt ttccggttca acaataatgt
    15421 ggtcacttcc gataacaata ttgaccgatt gatctcgttt ccgtatttca aaatttctga
    15481 tgatattgaa taatggtaca aaattgtttt tggcaatgat gtatccaatt gcgtcgttgt
    15541 tatttaccgg tgcgtagttc gatggtactg tcattgatgc ccgcttcacc acttcacggc
    15601 tttctgctgt ttgcagttca aactctatct gtttttgatt gttgattttt attatacggc
    15661 tggttataat ataattcggg tttaaatgcg aattgattgc gccttcaggt gctgtaacaa
    15721 tggtgctgaa cacaaccgga tctccgctgt ccgaagtatc ctgaacgaca tggcaatcca
    15781 gaattgttat gcagccgatg cttttgtcat tgtcatcaag cccttgtttg aaaaaacctg
    15841 gttcaacgtt taatccaaac gcttcttccg gcgaaacttc cggtttgata tcatagataa
    15901 tcagtcttgg tcttgggcaa tcatcgcatt tttcctgtgc cttgagggtt gaaatcattg
    15961 atgcgaacaa aaacatggct atgccataaa gaatttttct catgtctgga cagattggtt
    16021 tattttgttg actagtgggt tgatttgtta ttcaggacaa atataatacc agatattata
    16081 atatcaaaat caatttctgc cgcttcacac acagaagtca atgtgaatgc aggtgttagg
    16141 gttgtgaaaa attctcaatc aggtattatc ggttaatcaa tctgaatttt ctctcccaaa
    16201 aacttcggct tctttccaaa tgcaaaatcc agcaacactt tcacacgggc aaatttctcg
    16261 cgcagtttgg tcttgaagct attgtaggcg ctcacttccg ccgcattgta tccgactgcg
    16321 tccaatccga gcgagctggc aatgtagagt gcgcgccggt tgtggaattc ctgtgatatg
    16381 atggtgaaac tctgttgtcc gaatatggca gacatgcgat aaaccgaatc ataggtgcgg
    16441 aatccggcat agtcgagata aatgtctccg gctttcacac cgcgtttcat cagctcgtct
    16501 ttcatgtcct gcggttcgtt gtaatcgctc cggctgttgt caccactaat tacaatcttt
    16561 tttattttca tggccttgta cagctcaacc gcagccacaa tgcggttctt gaaaaactga
    16621 ttgatctgtc ccgatttcag atatttcgat gttcccagca gtaatccggt tttgttgtat
    16681 ggaatcgttt tcacatcgct gtacacacgt tcgcgcgtgt ggctgctgat gcgcacattg
    16741 gcaaatatca ccagccccag catgagcagt agaaagatga gaatgaggcg ggtgaggcgt
    16801 ttgcggagca tattctgata tttttactcg catgaacgga aaatttcttg ttttatttca
    16861 gatctgtatc cgatttcagc atccagcctt tgatgtgtgt gggttgcccg gtaaatgatg
    16921 ggtaataaca attcattttt ccgggattga catccataat cacatatacc cagtcaacac
    16981 cttgctcttt cctggtgccg agcagtttcc ctgtggcaca tttgccatat tttgctatgc
    17041 ttgttccaga atagtgatat ggtgggcaat cggtttcatc cagcatgtag cataccattc
    17101 tcaagtgagc gctgtcattc actatggaga aatccatgct gtcctcagtc aatgaatcgg
    17161 gcttttgagt aaaggaaata ttgcctattg tcttaattcg gataaatgaa ggtatgttgt
    17221 ttttgtagtg cgcagtgtaa tagtaggata gatgctgcgg atcggcacaa catcccgaat
    17281 tgtgaagtgt aaacgagacc agtctgttat ctttcaacgc atactcttta atttcctgta
    17341 catctgaaaa cacctccatg taatagctcc ccatgttgat gaagattttg gtcatcattg
    17401 gtgaaccgcc cgaagggccg tagtatgata gtcttttact gaatctccgt ttaggtcgat
    17461 ggcaaaaacc gtgtttacaa aactttccag aggtaaacta tagtaaggta gaatttgctg
    17521 aagttctttg ttgcttttaa taaaatgcat attttgaaat gaatcacgat cggtttcgag
    17581 ttcgctcagt ggtgtattag tagcaaattc gtcagttatg tattctatgc tatcatccca
    17641 ttcaaaaagt ttttcgcacg ctaccgaatc ttcaaattcc ttttgactca gcggtgtatg
    17701 gggctgctgg ccgctggtga agaggaaagc gaataataga aacaagaata aaaataagcg
    17761 attatatttg atcatatcag agttgctttg ttttaatgag tgggtgaaag cagcattcat
    17821 atcacaaaac tcatttgcct cattgtacat gcaaactatt tttttaattt ctttttagca
    17881 atatccttat cggcagattt cacgcggcgt tcgagttttt tgatgtcttc ttcgggtggc
    17941 aggtcttcag gtttgatacc gcttttacca aggagatggc gcacatcgcg gttgtttttt
    18001 acatgctctt ttgtaatgtt tgtttcgcct ttcagatcgt ctttctttac attgaaattg
    18061 gttatttctg tagccaggtc ttttgccttt atggtaattg taggcaggaa atcggccaga
    18121 ggtctgtttt ccggaatttt caatttgcgt ttcattgaaa gcgtattacg gccgccaaac
    18181 agcgcttcgt cgcctttgct gcggatattg gcaaatcctt tattgtcaac gccgcgctca
    18241 taaatatttt ttgaaagttc tgtttcagta gccactaatt tttcgcgtgc ctgcaagcgt
    18301 tcattcaggg ctatgcgttc ttcgagtagt tcctgcttac gggtttgtat ggcaaaatag
    18361 ctttgggcaa aagcaatctg ttctttttta ggatcaccgt tttgtgcgat cagatagcat
    18421 gcatagcggg ttagcataat atcactcacc tctctttggc ctcccttgcc tagttcgatc
    18481 attttgttga cgtcaacgaa atgatcaacg acaagctggg cgctggtttc gcacgattgc
    18541 tttgcttttt caatggcatt ggtgaaatta cgccaatcgg aatatccgag caattcctgc
    18601 aattcgcggg ctagccagta ttcaattccg tcctgctcat aagctgcttc ttcaaaaagt
    18661 ctgttcaatt gcctgataag agcattatcc attttgaatg atttttatat aaacaaatgt
    18721 aagagatttt attttcgggc agctgagaaa agtgaaatta tttcatcatc atatgattga
    18781 ggctaacgaa agcaggatta caactaagga aaagattatt ttgggaataa ctcaataata
    18841 ataattcact tttaaacctt atttttgcac caaatttcat ccgcatgaag aattttgttg
    18901 aagaactgaa atggagaggc atggtgcacg atatgatgcc cggtgttgaa gattatctga
    18961 aagaaggcat gggaacggct tacgtcggga ttgatcccac cgctgattcg ctgcatatcg
    19021 gccatctcgt atccgtaatg atgctcaagc attttcaatt gtgcggacac aaacccattg
    19081 tgctcgtggg cggtgctacc ggtatgatcg gtgacccgtc gggcaaaagc gaagaacgca
    19141 acctgctcga tgaaaccacg ctgcgcgcca atcaggatgg actgaaaagt cagctgatgc
    19201 gttttctcga tttctctgaa tcggctccca atgccgcttt gatggtgaac aactatgact
    19261 ggatgaaaga acacactttc ctctcattca tccgcgacat cggaaaacac attacggtga
    19321 attacatgat gtccaaagac agcgtgaaaa agcgcctcag tggcgaagca ggcgagggaa
    19381 tgtcatttac agaattctcg tatcagctgg tgcagggcta cgattttttg tggctttatc
    19441 agaatcgcaa ctgcaaactg cagatgggtg gcagcgatca gtggggcaat attaccaccg
    19501 gaaccgagct catacggcgc aaagccagcg ggaccgcgta tgcactaact tgcccgctaa
    19561 taaccaaagc cgatggtggt aaattcggaa aaaccgaaaa aggcaatgtg tggctcgatc
    19621 ccgaaaagac gtcaccgtac gcattctatc agttctggct caatacttcg gacgccgatg
    19681 cagaaaaata cattaagatt tttacactga ttggcaaaga agaaattgaa tcactgattg
    19741 cacagcacat ccaggagccg catctgaggc tgttgcagaa acgtctggca aaagaaatga
    19801 ccatttttgt tcattccgaa gatgattaca atacagcggt cgaagccagt gaaatcctct
    19861 ttggcaaagg aactacagag actctggcca aattacctga gtctgttttt ctttcagtat
    19921 tcgatggagt acccatgacg gaagtgcctg catcgagctt gcaggaaggc attccggcga
    19981 ttgagtttct gtccgatgtg gtgggcgcat tcacgtcgcg cggcgaggca cgtcgaatgg
    20041 tgaaagacaa tggtgtatcc atcaacaaag aaaaaatcaa cgaggaattc gttgtaaact
    20101 ccgcattatt gctcaataat cgctatatcc tgattcagaa agggaagaag aatttttatc
    20161 tggtgaaggc ggtttgaata ttgaacacgg aacaaggaat tgttgaagta agaaggaacg
    20221 ttgtgaatat tgaacacgga acaaggaatt gttgaagtaa gaaggaacgt tgtgaatatt
    20281 gaacacggaa caaggaattg ttgaagtaag aaggaacgtt gtgaatattg aacacggaac
    20341 aaggaattgt tgaagtaaga aggaacgttg tgaatactga acagggaaca aggatttttt
    20401 gaagtaagaa ggaacgttgt gaatattgaa cacggaacaa ggaattgttg aagtaagaag
    20461 gaacgttgtg aatattgaac acggaacaag gattttttga agtatgaagt aagtctgaag
    20521 tgaatattac ttcaacactc gcacttccct gtttatgatt tcgcatttac atcatcattc
    20581 acacttcctt gcctgcctgg ccgtcagaca ggttcatgat ttcgcattca cttcatcatt
    20641 cacacttcct tgttcattat tccttgttca ttattccttg ttcatgtcat ctccacttcc
    20701 agtccttctt atcatacact gatttgcctg atttcagtgc cagttgtttg atgaaatcat
    20761 cccacacatt gcggccgact tcgcgtccga tgggtagtat ggcaacggtt ccggtgtcga
    20821 ataaaatcag ataatgcgat ccaagatcat tgatttcggt gatggatgaa tatttgatta
    20881 cgcgctgacc ttgcggtgct gtgagcaaca aatcgcttga acgagcttca acactcaatt
    20941 cttttccaaa attttcggta tatttttcct tgatttcctt tcggaaatga tttttataaa
    21001 aataccggct gtatattgga tatgtgaaaa accagatcag ggctactcca gccatgatgg
    21061 ctgctccgac ccataccgat tcaacagcga aataaatgcc gatggcgaga aacacaagcg
    21121 gccatacaat gcgtgtcagc atgcgtcggg ctttcacctg ctttgagcgc gaagctgtgt
    21181 ataactgaaa tttgaggtag tcctcggctg tgagctgata attgattttc atgcgtagtg
    21241 gattaagatt ttttctgcgt ttccgaaaac ttcaaaattg tcgtagatac tgatgtctgc
    21301 atcggtcaga ctatccttga aagaaatggt gtccttgtga tccggattcc ataaaatgag
    21361 atttgatggg attccggctt tgatgtagcc gtaatcttca aaaccaaaaa tacgggcggc
    21421 attggtcgaa gtgagttgta caaactgctg catggtgatt ttgttttgca gcactccgtg
    21481 agtgaaaagc aattgcatcc ggtaatgcag tccccctgcg ccattcggaa ttttggtgaa
    21541 atcgaaacgg ccttccattt tttgccttaa tgtgaatgga cagtgatcgg tcgatacaca
    21601 gtcgatggtg ccatcagcaa gtcctttcca gagttcttcg cggtctgcgg caccacgtat
    21661 cggcgggctg atgatgtatg ggaaaaccat gtaataaaac tgctcataga cttctttatc
    21721 cagcaacaga tattgcgggc aggtttcagc atataccgga agtccttctt tttttgcttt
    21781 gcgtataagc cgcacggatt ctgcagttga catgtgtaca aaatatgttt tgcaacctgt
    21841 ttcgcgcacc agatcaatca ctttttccac agcattgtat tccgtttcag gtggccgtga
    21901 gtacgcatgg tatttgggtt ccagctttcc ttcaatgatg aatttattct gcagttcctg
    21961 aataacatcg ccttcttcgg catggatggc cagcacagca ccatagtagc tcagacattt
    22021 catcacttct ttcagctggg taaaatccag accgatggta ttcctgtatg ccagatatac
    22081 tttaaatgaa gtaatatcgt actcctcaat gcattgtctg atttcttcgg ccactgcatt
    22141 gttccaccag gtaatgccca tatggaattt cagtggaatc gcacaggcaa cggattcgtg
    22201 ttcgcgcgca tcaagcgctt cgatcaggga ttgtccgcgt gcaggggtta caaaatcgat
    22261 gatggcccct gtgccaccca tcatggctgc aaaagagcct aagccaaaat cgtcgcatga
    22321 ggggccggcg ggagtcggca gtttcagatg cacatgcgga tcaattccgg ccggaatgag
    22381 gtagcggcct gtgccttcaa agatttttgg tgaatttaca tccaacgtgc cgggtttacc
    22441 aatcagctcg atgatctgat cttcgttcac atatacatcg gcatcgaagg tcccgtcagg
    22501 gtttacaaca gtgcagtttt tgatgaggcc ggtatccatt tttttgaatt gaaggattaa
    22561 aattacaatt attctttttg gtttgaaaaa tcagaaaatt ctgaacacag atttttatgg
    22621 ttgcatatcg gaaatcatcc tgtcaatcag ttgtccgagt tccggaaggt ccttgtttat
    22681 cgtatcaaac accattatca gatcgacgcc aaaatattca tgtgccagga agttgcgaaa
    22741 cgagcggggt ttgtaccatg gatatttgta cttgtccgtt atttcgacag gcagcgaagc
    22801 aatggcctcc ccaataatca gaaactgata gcaaacagca ctgctggcag ttatatcgga
    22861 aataaattca tctcgggtca aatcttttga aatgagctca attttactga tggcatccat
    22921 aatatgctga agccggtacc tgtatgattc tttacttttt ttcatacagg actttcagat
    22981 tatcatggat ggattcctga attttaggtg agagcgcgcg ttctgtgaca acatcggttt
    23041 tgcgtcccaa caccttttca gtcttatgtg caatttcggc caaatcgaac agcgtgaatg
    23101 aaaaacggtt gtctgttttg atcagaatat cgatatcact gaaatcggtg gcttcgtttc
    23161 tcgagaagct gccaaagaga aatgctttca gaattctttt atccttgcgt aatatggcag
    23221 aaactttttg ttttatttcg gcaaacgtcg gcataacggt ttgggcataa accgataccg
    23281 gctcattaac caatgttctc gattcagtga gttcgcggct ccacttgctt tgcatccaca
    23341 ttttcagcag tgcagcttca tcgagggaat aatataccgc gagcttgatt acaagatttc
    23401 tcgatgccag tcggattccg cgctccactt tgctaagaac agctgcatcg gcatcagtat
    23461 cagctgccac ctgacgcagc gtcatgccac gcgaagtgcg ttgatttctg atataggtgc
    23521 aaaatgactc cataaaaatc ctgttgacaa attttgtcaa atatacaaaa agaacttgtc
    23581 agaaccaaga aatcagtaaa gatttcacgt ttgctattcc gacatcttgt cccaaagcat
    23641 ctgcgcggct ttgcgcgatt ccttcagaat ggagtcttca tctgcggtca ggatatttcc
    23701 ttcgctgtaa atcagcttgc cgtctgcaat ggtgtgtttg acatgacgtc cttcgagtcc
    23761 gaaatagaaa tgaccgagga aattgttttg agtaaccgga gtgggtgagt cgtagtcaag
    23821 aatcacaaga ttattgcttc catgcggaat gttgctttca cgcagatatc gggcgccgtt
    23881 gcgcagacgt gtataaatgt caatccagct cacgttgtcg aaattctgtc cggcaaaaag
    23941 cgctgtctgc gcactcttga tcatgtcgct gtgcatgccg tcagtaccca gcatgatgcg
    24001 gtcgcccagt tccgtggaag tgaaaaaacc cacccggttg ttcaggttgc tgtcgaaatt
    24061 cacggccatc caggtgccgc tttcgcgcac gagtgtccgt tccttcacat ccatatgcag
    24121 accgtggccg aaaatattat ttttaagcga tagtgctccg gcccgctcca gacgctgcac
    24181 aacgctgcat ccgtatttct ctttagtgaa tttctcatcc atcggatctt ctgcaacatg
    24241 aatatgaatg ccggtcttgt gcttatctgc aatggcaaca gatttcagta atgtatcttc
    24301 ttcaacggtg aaagacgcat gcagtccaac aagcccgcgg cgactattca aatactcatc
    24361 gctttcttcc agactttctc tggtcttttc aatgccgtag cgatccgtaa tttcataaca
    24421 caataaatgt ccgacaccca cttcatcaaa agcttttgca atggtttcga gggatccttt
    24481 cagaaaattc ggcgacgcat gatgatcgat ggcgaaggtg catccgtgtt tggcacaatt
    24541 cattgcagtg gtgagcgcgc ttaatcggac catgtcctga tcgaggcact gatcaagagt
    24601 ccaccagata tattcaagcg tttctgtgaa atttgtcggt gattttttag gtgcgttcat
    24661 tccgcgtgac aacgctgaat acacgtgatg atgcgcgttt ataaatgcct gtgtgaccaa
    24721 aagcccatgg cagtcaatgg ttttatcggc ttttgtgtct gcgggaacaa actgaaaacc
    24781 ggcacccgga gcttcatcca caagcatgtg tcctgttgtg atttcaaaag tttccggatg
    24841 aatatacgtt gcgtttttta ggagtatcat gattcaagtg tttatacaaa tgtagtcaat
    24901 aattcaatgc ggatgcggtt ttcaatagag aaatccgaac agccagagcg gaaccacatt
    24961 ggtgtgtcca tattcaatat cgtcctgaac aacaaaagca ttgcggattc cggcgatctg
    25021 ggttttcttt tttgactttc cgccgatttc aaatgtgtat tttccatcca caagaaaatc
    25081 gcttacaggt gagatattga cagtgtgcaa cgctgaaatc tggttcataa aaaatgtttc
    25141 gcgtatggtt cctttttccg gaatccgtat atcggcgagt gcaaaaagca gattcgggtt
    25201 ttgcagatac accttttccg gcttgctcag aagccccaat ccttttgttt cggcatacag
    25261 cgaagcgaaa atttcggctc tggaaagata ttgaatgtag cggataatgg tttcgcgcga
    25321 caatcccatt tcacgtccga gttcagtgat attgggcttg aacggtacca gcccggccat
    25381 gatggccagc aatttccgta tggcaacaac ggcagtgtaa tcaacattaa acagcgcagg
    25441 caaatcggtt tccacgacct gattgatggt gctgagcagg cgcctgtgat agccctttgt
    25501 atcatcgcgg gcaaaggggt agctgccggt ctgataaaat tcatgcaggt accgtatggg
    25561 gaaatcgact gttttcatca gcttttcagc tgtttttgtg cttcgtagca gaatgtcttc
    25621 gagcgaaatc acaggcgcct gaatcccgta aaacaattca atgtattcgc gcagcgacat
    25681 ggaattcaga tgaaattgca atgcacggcg gctcagatca gccccgcttt tgtatatatt
    25741 aagtgccgat gagccggaga atattaccgt gagctttttg taccgatcgt atatattttt
    25801 gatttcgcgc gaccagcccg gatatttgtg cacttcatcg agaaacaaat gtgtaccccc
    25861 ttgctttaca aaggcattgg ccaatgaaac aatagtatta ttgccaaagt aaaggtcatc
    25921 gcagctgaca tacaaagctt tgtcactgtc gctgaagttt gctttcagat actgaagcat
    25981 cagcgtggac tttcctgcac cacgtgcgcc ggttattccg gtcaaccgct gatcccatct
    26041 gactttatcg agtgcatagc ggataaaggt catatccgta tcggctatgt attggcgcga
    26101 tgtttctctc agtgtttcca taattgtaaa atagaattag caaatatacg aacaaaagtt
    26161 gaatagaatc aacaaaataa caataaaaag taaaataaaa acaacaaaat acaagaaaaa
    26221 cgtaaagaga aatcaacaaa aaacgccatt attgacttaa tatactgcaa atcaacttga
    26281 tatatatata ttaaatgata gaaatcgtaa ataacacgaa atcagccgaa tagatgattt
    26341 tgtgatattt ctccctattt ccgcataggc aattgcgttc tgcgaatgcc gtcgaaatgg
    26401 cacagtgcat tgccgactgc tccggccgta ggcaccaggc cgatttcacc cagacctttg
    26461 gcgccatagg ggcctaccgg atcgggcact tcaactccga tgacttcgat ttcaggtgtt
    26521 tcatgtgctc tcagaacacc gcattcgcgc agctttgaat aatcaggata accgtttttg
    26581 tatggataat cttcgctgat ggcgtatcca aggcccatgt gaaccgcgcc ctcaatctgg
    26641 ccttcgaaca tcactggatt gataattttt cctgcatcat gcgcagcaac tattttttct
    26701 atttttccgt gatcatcaag aataaccagt tgggcagcat atccgtagcc gtaatgggtt
    26761 tttgaatttt cgcagattgt gccgggcttt acggtccatg tgcattccca gcgcgcctga
    26821 tagcgatgtc cgacaatgtc ggccagtgtt tttccctgca aatcggcacg catggtttta
    26881 gctgcctcga gtattgcatt accaagcagc acggtaccac gcgatgaagt tgtcatgccg
    26941 gttttgatgc ctgctgctgt atcaacaatt acttcaatga cagaagagtc aattcctgtt
    27001 tcttcgcaca gaatctgggc tgcaacggtg tgaactcctt gtcccatctc ggtccagcca
    27061 tgatgcaaca ctactttttc gggcgatttg atttcaatga tcacatcgga ataatccacc
    27121 attccatttc caactccgct gttttttatc ccggttgcaa gaccggaaaa cctggctttg
    27181 tcataataag gtttcacagc ttcaagacag gccctgatgc ccacgccttc cacatggtcg
    27241 ccggttgcgg tggtcagtcc atcgatcaat gcattatccc atcggaactg ccagcagtcg
    27301 aatccgccct gcttgcatag ttcatccaca catgactcga gtgcaaaggc ggcctgattg
    27361 gcaccaaacc cgcgcatggc gccacaaggg acattattgg tgtaaacagt ttttgcttga
    27421 aggtcaatta ctggaacaaa atagccaccg gttgcatggc cggcaatgcg ctccatgact
    27481 ttggttccga cagatgcata tgctccggta tcaccaactg cactgaattt catggcgctt
    27541 agttttccat tttcatcgca ggcgagagta atgtccatcc agaccggatg tcgtttggga
    27601 tgcataatca tgctttcttc gcgggtaagg gtgagcttta ccggtttttt cagcaggaag
    27661 gcaaacaaag aaacatggcc ttgaacagtc agatcttctt ttccgccaaa accgccacca
    27721 ttgggaacaa gaatgacacg aactttttct tcactcaaac ctagtactga tgcgacttgt
    27781 cggcgatctt cgtaaatgcc ttgtcccgat gaatacagca gaacaccgtc gccatccggc
    27841 attgcgagtg cactttctgt ttcaagaaat gcatgttcaa tacgctgcgt ttcaaaatta
    27901 ttgtgataga catattttga ttttgcgatg acttcatctg cgtttccttg tttcagaatg
    27961 cagttttcca gcagatttgc gtgggtatca tgcacttgtg gtgaggaatc tttcagcgct
    28021 tcgtgtgcat cggacacagg tgtaagcaca tcgtattcaa tgatgatttt tttcgcagct
    28081 tcacgtgcat tttcagcagt atcagccaca acgcctgcca gtacgtcgcc tatatattta
    28141 gtggtttcac ccacagcaat catcatgggc cagtctttgt aaatcaaacc gacaatgcgg
    28201 tttccgggta tatcagcagc ggtaaaaacc tgttcaacac ctacgctttt caatgcttcg
    28261 gaggtatcta tttttaaaac ttttgcccgt ggatgatcac tgaaacgaag tgcaccgtgc
    28321 aacattccgg ggaagctcaa gtcgtttaca aattttcttt gaccgatggc tgtttcaaat
    28381 gcttcgtatt ttggcatgaa atcaccaacc cctttgtggt gattgtcgtt ttcgcagctt
    28441 ttgttttcag caattaattc agctgaatat tcaatggctt tttcgatttt cttatatccg
    28501 gtgcagcggc acagatgcca ctttaaggca gttctgatct catcaatggt tggatctgga
    28561 ttttcctgta gcagaatttt ggtgcgcatc acaaaaccgg gagtacagaa gccgcattgc
    28621 acagcacctt tttcaacaaa agcttttgca atcagatcgc gcactttggg tggaatgcct
    28681 tcaagagtct ggattgatgc gccttcgagt gagcccattt tggttacaca actcagtttt
    28741 gccttgccgt caatttcaac cagacatgca ccacaggccg attgaccgtt gcaaccgtct
    28801 ttaaccgagg taataccttg ctcatttcga agccagttca ggagcgacat ttcagcattt
    28861 ccgccgaatt caaccggttt attgttcagg acaaacttga tcatattttg tagttcaaat
    28921 caaaaaaaca ataatttttt atttcaaatt aaaaatctga aatctccaat ctatattctg
    28981 aaatcattca gaaactcttg cggattcact gcttcaggga tattaataac aggctgaact
    29041 gcattgacat ccttaagacc gctgccggta agaaggacac aaacattttc gtttgcagaa
    29101 attttttctt cagtgatttg tttcagcaaa cctgcaaaag ccgttgcagc tgcgggttct
    29161 gcaaaaacgc ctgtgttgga actcagcgat tttgaagcgc tcagaatttc cgaatcactc
    29221 acttcaatgg tttcgccctg atattttttc aggaatctcc gggccataaa atagttgcgt
    29281 ggaatatcaa ctgaaattga gtctgcaaca gtttttgacg gcaatgagac aaatgaatct
    29341 gaatagagat ttctgacaat attattgctg ccttcggctt gcaccgcaac aatcacgggc
    29401 attttgtcaa tgagttcaag tttcagaaga tcttcaaacc ctttatagac gccggaaatg
    29461 atcacgccgt cgccaaccgg aacaaacact ttatcgggcg ctttgtttcc gtattgagta
    29521 aatatttcaa acgaaacggt tttctttcct tctatggtga aggggttgta tgctgtattg
    29581 cgattgaata ttcctgtttt ttcagtaagt gcaacactga gatcgaaagc gtcatcatat
    29641 gttccggcaa ccggaacaat ctgcgcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29761 nnnnnnnnaa gtttgaaagg aagtttctgc tcattcagtt ccgacaccgg aatcaaaggc
    29821 gtattgccga ctgatagatg cccgagtgat ttggtttcat taataggtaa caagggcaac
    29881 catccctgag aagtgaagaa tgaatcttgt ttatgagatt gatttttcaa ttcatcaaaa
    29941 tcatagatcg ttttcagaac tcctttgggt ggttgtccct gcgtattctt cgcagcacaa
    30001 aaagggcaca gatacatgaa actgacatca tgaaattctg caccgcaatc ggtacatttg
    30061 aaaaaatatt tgcgttgcat atgcctatgc ttttttcact tcctgattga attcctcaat
    30121 catttcgtcc cattttgcgg tttgagcaaa cagatgattc cagatatcat ggtcgtacca
    30181 aagctgattc agatcttcca gtggtttttc ctgttgttca atccaggtaa aatatttcag
    30241 gttatgaatg gcttttttgt cggagtaggt gagttctttc agataatcga tagatgcagc
    30301 gagcatggtt ttttcaaagt cacggccggc ttgcagattg gtataagcgc cacgttcctc
    30361 attcagttcc ttaatgcgtg aatcgtacat ttccatgctg tcggtagcta cagtaatgat
    30421 gacatcatct tcattcatct catagtattt tgccgttttg atggcagaaa tcaggttcgc
    30481 tacacttgag atgccaagca gatcgaggcg gttaataaca tcatcctgaa caccaatgct
    30541 tttcaggaat tttcttcctt cgggttcatt aaacagacgt atagcgcgca tacagtcttc
    30601 atcatcgatg gcagtaacaa catcagtgtt ttttacattg tgtacccatg gaacatgctt
    30661 gtcgccgata ccttcaatgc ggtgtccgcc aaaaccgttt gctatcaggg tcggacattg
    30721 cagtgcttcg gatgccacta ctttgatatt cggatataat tttttgagat aatcaccggc
    30781 ggcaatagtt ccggcgcttc ctgtgtttga aacatatgca gtgagacgtg agcgttcatt
    30841 tttttcgcat ccgaaaattt cttcaatggc cggccccgta atgctgtaat gccaaacagg
    30901 atttccaaat tcgtcaaact gattgaaaat tacgtgatca ggttttgtgc gacgaatttc
    30961 ccagcattta tcatagattt ccttcacgtt tgattcgcat ccgggagtgg catacacttc
    31021 ggctccgatt tcgtgaagcc attcgaaacg ttccttgctc atttcctgag gaaggatggc
    31081 aacagcagtg cagcccatca gccatgaatc gaaagcgccg cctcgacagt aattgcctgt
    31141 gctgggccaa actgctttgt gaaattcagg atcgaattgt cctgaaataa tgcgtggtgc
    31201 aagacagcca taggccgctc caactttgtg agctcctgtt ggaaaatatt ttccgaccag
    31261 catcaaaatc cgggctttaa cgcctgtaat ttcttttgga atttcaatgt aattgacttt
    31321 cccaaaacca cctccggttt ttaccggctc atttttccat gtgatacgga aaaggtttga
    31381 agggtccaga tcccacagcc caatggtttt caatttttct tttacctctg ccggaacaag
    31441 ttccgggttg cgcatttgtg cgaaggtagg aagaataatt cctttttctt tgaatctttt
    31501 agcagctttt ttacgaatgt cggcattgac aacgtgatca agaatctcaa tcatagtaca
    31561 ttttatttaa gtctgcaaag ttaggcattt ttaaaaacag caatctgtta aaactatatg
    31621 taattcactg atattggatt aagtagatgt atttgattta ttttgtgatt aattaatgaa
    31681 atacagagat tggttgtggg gcatatttat attgtgtaag taagtggctt cagaaagttc
    31741 aatagtttta atgtaatttc tttgtcattg taattctggg taatcaagcg gtagccattt
    31801 tctgctattt tcgatttcac agaggggttg ttctttaatt ccataatgga ttcagcaagt
    31861 tttccggcat ttgcttcaat gcttacgata tttgtacccg gcgtgaatat ttcgttgatt
    31921 gcatccgatt ttcttgtgat gatgcatttg ttcatgactg cataatgaaa cagtttgttt
    31981 ggtatgacac gctttgcttt gcccgagtga ccaaaaatac caagagcaat atcaaaattg
    32041 ctgatggctg ccggtagttt ttcatatggc agccatccat gcaggtgtat attctttaag
    32101 ttgtactttt taatatccga taaaaaatcc ttgtaagtgg cgccatttcc tatgatgtca
    32161 aaaacaatat tttcatttct gagtagcaat gctgtttttg caatgacatt ggttccctgt
    32221 agaggaacaa aactcccata aaatccaaca tgaaaaagcc gatcttgttt ttcctgaatc
    32281 gaagctttga aatcatccgg aataaaacca accgaaataa cggctatgcg ggtttcgggg
    32341 aaatgataaa acctgctgta atattttttc atggcttctg tatcggcaat cagataatct
    32401 gctgcatgga tggctttgaa gtcgagcaga tattttgtgg gggccttgta ccattggcca
    32461 taatcgacga ctttggtcag gtattctgaa atcaaaggat cgaaaaccac aggggctttg
    32521 ctgtgctttt ttatccatgc aacatctttg tgcctgaatg ccggaacaaa aacaaagtcg
    32581 tgttgtgatg attcttgctt taattttagc gcatgatttt tatcccgtga gctaattttt
    32641 aaaagattga catgaacgtt gtcaagtttg ccaagaccgg ataaaatcac ttttgttctg
    32701 tgatagctga atgtgtcttt tccacagaat aaaatatcag tcatgttttc aggtcttgcg
    32761 gttattaatc aaaaagtccg tctgtcaacc agtaaatgga tggattgccg gctcttattc
    32821 cggcaaatgc ctgcctgaca taaatattgg ttgtattttt gttgcagaga taaacccgga
    32881 tttttgcgtc aaaaggaatt tttctgatat ccggaaaaaa caaacctgat gataccggca
    32941 cccatgtatt ggttgggagc aggtcggtgt ttacggatgt tttatttacc gtaataatac
    33001 tggtatcgga tacaagttcc gttacccaga atgcctcctt ttccaaagtg tcgggcagat
    33061 aaatccagat ggttgcatca aaaatattgg cctgtgatct taaataaccg ctgatatgct
    33121 gtgtccatga aatacaatgc tggttattgt tgccgcattt caggattttg gcggctttga
    33181 gtgtatctgt cagaacatgg cacgtgtcaa attcccaaca gacattggaa tgtttgctga
    33241 aattgcaaag actgccgcgg agatagtttc tgtagtgagg ttcgcctctt ttcattacga
    33301 tgcaagagcc ctgattgtaa tgagattcct gcagtgatac agaaaaatat tcatgcgaca
    33361 atgcataaag cgacatgggg gtctgagcat tttctgcaat aatcagataa tgatattgtt
    33421 catctgagag ttgtttcaga aggtctgaaa aatctttatt tgagcgataa aagaaataat
    33481 gcagtttgtt ggcgacacca tatttatctt tgtagaagtc tgcgattttt tgatcataag
    33541 agcacaacac cattattgaa tcaagtgagt gcgtttgggt aaaagctttt atctctctac
    33601 ctgtttcttc gtacggcgaa tggtaaaagt attggtaatg atcgcggctc cagattagtg
    33661 aatgaataag tatgagcgac catattacag tgattcctat gacatgccga aggtttattt
    33721 tttcgtagaa gctgaacaca aaaacaatga tcaaagggaa tgaaaacagc atcagagaat
    33781 atctgttgaa aaacggatag ttgtttgaaa ataaaaagac agaagctact gaaatcgttg
    33841 ttaaaaccag caacacgaca tgaaatttcc caaagcgtat ttttcttaac agggctgcag
    33901 cgaaaaaacc atatagtgca atcagtacaa atgcgcctgc tactatccat gagaaatcga
    33961 aagcatattt caaaaaacca atagaataat ctgtagccga agtaactgaa acgtgattga
    34021 actcagttat tttgagctga ttgagagtga gaggaatgtg agggacaatg gcaatgatta
    34081 taatgatgca agcaatcagg tattttttca gatcttttcc tctcaggtaa aacagaccag
    34141 tgagaataag ggaaacaaca ataatcagcg aaaagtagtg aatgtagctg cttaaaatgg
    34201 ctcctgttat aaataaaagg aaattgtacc ggtaattatt tgttgttagc agaacgaggc
    34261 gagcccagcc aagtagcatg agcaaaacaa agaaaaggcc tccggcatac ggcagagcca
    34321 tttggctgtg aataataaag aaatgcatga atgccagcgt acttgcagaa agcagtccaa
    34381 cagcaggcga gtaccattca cgaccgattt tccacatcag ataaaccgaa gcaattccgg
    34441 ccagaatgaa ggggagtttt atccagccct catttagtcc ggttagttta atgtagaaaa
    34501 aaagcagcac ctgaattcct gccgggttgc tgtcatttgg aataacatta ttcagcaatt
    34561 ggctgaatga aatatttttt gctgttaata taacattcag ttcttccgaa gaaaaaggaa
    34621 tatggaaaag gttgaacaat ctgagtgccg ccgccgcaac aacaatgaca agaactaaaa
    34681 ttttcaatcc cggggttact ctcatttcgt tgtatttttc tgtttgattc cgaggtgatt
    34741 tattaccgga ttggcatact gaatcaggat aaaatgcagt tcattctcgc ttaatcctac
    34801 attgtttaat tgctgaatga aataattttt tatcctgaag gcactggatt ctgcaatttc
    34861 aaatgtgttt gtttctgaga gcaggttata agcaagtttg tgtgtgtcat acagcgtata
    34921 gtttgaaaaa atcacattgt cgatcatttt tgcaatagct gcactttgtt tcttgttgtc
    34981 agtttcagga gattgttgta tcagttcatt gatgggttct gaaaaatgaa aattcacttt
    35041 acctttttta cccccaatac cggtaatcat gttttttaaa tcatcctgcg gtgtttttac
    35101 aaatggtttc ccaatagctt ttgttcgtag ctccagagct ttcaatccat cgcatggatc
    35161 atattcgtat gaaattgaaa cgggagaaat attgagaggg gtcagtgcct tgcgaagatc
    35221 tttgctgttt cctgaaagtc caagcatgcg cagaagactt tcctgggtgc ggtcgttgcc
    35281 gtctttggca cggccttcgc gttgtgcaat ccataaatag ctgctacgat ccacaatgct
    35341 gtgccggatg tattctgaaa gcaaaacaga gctgttcagc aggtcgcgtc ccgacaaaga
    35401 cctgcgaaca ataaaggatt tgtttatttt tacgaaagtc tcaatccatg gtttaatcaa
    35461 cagattgtcg ccaatggcga tttcaacggt aggtatattg ttgaaatgca gccccagatc
    35521 aatgagcgac ggatccatca caatatcacg atgatttgat ataaaaagac agggttgacc
    35581 ggcggacagg ttttctgctc cggtaaagct gagatcggta atggttgaat tgatgatgcc
    35641 ttgcagtatg ggtttgatga gcagaacctg aaattcgtca attgttttta catcctgcag
    35701 tttttcgaga tgcaaggtgt atttctcgtg aaaaaaatat ttcagaacag aaataaattc
    35761 ttcttcctga atgagttgat taaaggccgc tttgaactct tcattacggt aaggacggat
    35821 atcattgaac ttttccatac ataaaccaaa ttacaaaaat aggtaaaaag aataaatgcc
    35881 atattttttc atgtttgaat tataattgtc aaaaatcagt atctttgttt taaactatgc
    35941 tgtatgcgtt gcttgtttat tgttttactg ctgtttgttt ttattccttt caatcaaata
    36001 tttgctcagg atgcagataa gttcaggatg ccagccggtt gtagctccaa cgattatatt
    36061 cccggtcatg taattgtgaa gttcagttct gatgtttcag atcgtgcagg taacagggat
    36121 tttcttgctt taatgcaaga gttgaatgta gtgcggtttt atcaaaagtt tccccacagc
    36181 aaaatgcctt cagaaaaagt gaatgagtgg ggtatgccga tggttgatat ttccggcatt
    36241 tatgagcttt gttttgattc atcggtttct gtggagttcc tgattaatcg tttgcttagc
    36301 gcaggacttt ttgagtatgc ctgtccgcat tatattgagc atcaactgta tgttcccgat
    36361 gatccgaata taggaagtca gtattatctg accaatatca gggcctatca ggcctgggat
    36421 atttgcaaaa gtgatacaaa tcgcattgta gctattaccg ataccggtta cgagtttaat
    36481 catcctgatt tgtcggctgc tgtaaaacac aattatgatg atcccataga tggtgttgat
    36541 aacgataacg atggttatac tgataatttt agtggttggg atatgggttc atgggataat
    36601 aatccgcaat atgcagcttc aggacatggc attcatgttt cgggactggc cggagcttca
    36661 gccgataatg gatttggaat ggccggtgtt ggatttaaga gccggctttt gcccgttaag
    36721 gttgataatg ccaatggtaa tctggttgcc acttatgaag gagttgttta tgcggctgac
    36781 catggcgcta cagtgattaa ttgtagctgg ggaagcactt attcagggaa tttgtatggg
    36841 caggatattg tcaattatgc atcttacaat tgcaatgcac ttgttgttgc tgcctgcgga
    36901 aatgccaata atatgaatat gtattatcca gcttcgtaca acaatgtgct gagtgtggcc
    36961 ggtaccgatg caaatgacta taaatcatcg aacagcagtt ttggttataa ggttgatatc
    37021 tgtgcgccgg gtattggtat atattctaca tggtctgggg cttcttttgt tacttcgggc
    37081 ggaacatcga tggcttcgcc gattgttgct ggtgctgctg cgcttgtttc atcatggtat
    37141 ccgcaataca atatgttgca gattggcgaa atactcagaa actctgctga tatcattgat
    37201 accatcagtg ccaatcagtc atacgatgga ttgttgggta tgggtcggct gaatatgcat
    37261 cgtgcgttga ctgattccat aacaccggct gtaagactgg tcgagtgggc tttctctgat
    37321 agcgccgata tggaatttga tcccggtgat actttgttta ttggcgcaaa ggcactgaat
    37381 tttcttgata catcttcttc tttgttgtat gcccggcttg aatgtatgac tcctgaagtt
    37441 gagttgattg attctatctg gatgattggg acgttaaaca ctttacaata tgccaataat
    37501 catttcgcac cgttcaaagt gaaactttcc aatgtccctc caagtaccaa tattgcattt
    37561 ctggttcatt tttacgacag cacctggcat tcggccgatt acatcagcac caacgttaac
    37621 aaagattaca acactctcga taccaatcag ctcacactta ctgttacaag tcacgggaat
    37681 tttgcataca acaatctggt gaatctgtcg cagggcgaag gattcagatt taacgatggc
    37741 agcaatctgg tttcgggttt tggcttcatg tgtggtactg atatgtttca tctggccgac
    37801 aatctgtata gcatcgtatt tccggttgac agtgattttg ttgctgacaa ttttgtgctg
    37861 gatctgaatc ccgctcccat gggcgaccaa tgtctttttt ctcaatatac cgatcagggc
    37921 gccgattcag cttcgcagat gaatattatt gtcaggcagt ggtcttatgc atggaatacg
    37981 cccgaagacc gtaattatat tctcgtcagg tatcaggtcg tcaatgactc gattgtcggg
    38041 ctggatgatt tttacaccgg tctttttgcc gattgggaca tcatcaattc cgccgaaaac
    38101 agatgttggt acgatccagc tatcgatgct atcgtcgcaa ccaccactga ttcggttatg
    38161 tttacagcaa cagctttatt gtcgccatat tccagtaagc attatgctgt tgatatggat
    38221 ggattcaaca gtagtattgc tttgaccgat ggtttttcgg aatccgaaaa atattcaatg
    38281 atgaaaacga accgcttgta tgccggtacc gatctggcgg gcaacgacat tgcagctatg
    38341 gtgagttcag gtcctcatac aattgctcct tttgatacgc tggaacttgc gtatgtaatt
    38401 gttgcgggaa caaatttcac cgacattgca gatgctttag ccagagccag aattcgttat
    38461 cttgatccgg atcttggtaa tgcaagtgat gacgcacttc cgtttcttat ttatccaaat
    38521 ccggctttgg ataggttgca ttattttatt ccggcaggca atgatcgtta cgatgtttat
    38581 atcagcgaca tgaccggtaa attatgcatt gaaaagcatg atgttgaaaa agaaggacag
    38641 tttgatgttt caatattgcc ggcaggttta tatacggtat cgtttgtttc aggcaaaacc
    38701 tgcagcgtta tgaattttac tattgtacga tgaccatgag tttggacaat aatgcaaaaa
    38761 gctggaacat cctgattccg tattattttg atgcattcag tgattatgct ttgcaggagg
    38821 gaattgcact cagtaaggtt tttggtgcgg ggctggtgtt gtttcactgt aatcctgaca
    38881 atactgtgaa aatccgtctg gcagagatgg ccaataatat catttccaat cacaatatca
    38941 ggaccgaatg ttttattccg gaagggaatc aggcggattt gctttttgca ctggctcata
    39001 agacagaatc aatattgatt attatcgggc acaattgtca aaaaacatct ttgggttttt
    39061 ctcttaaaaa gaccattcgc attatgcgga aatcccgaac gccttttttg atggttccag
    39121 agggtcttca tgaaaaatct ttcagggata ttgtttattg catgggatat cagaaaactg
    39181 aaaaggagaa aattttgtgg gcatcatatt ttggacgtat atgtaaaagc cggattcatg
    39241 tggccatgcc aaaagtatcg gatcagtttt tcaaaacagg cataagatct aattttcagg
    39301 cgatgaataa gttgtataat gcggttgaaa tagatttcaa aatggttgcg atggagtcga
    39361 atatacatca tgccgtaagc gctgccattg aatacggaca ttcaatccag gcaggtgcat
    39421 ttattatttt aacaacgctg cgacctgata ttttcgactt ttttggcgga gccgatgata
    39481 tagctgcaat ccgtaataaa tattcattgc cggttttttg catcaatccg cgtgatgatc
    39541 tttatgtgct ttgcaattag accttgccgt taacttaatt gcagcatact ccgactgatt
    39601 ggcggccgaa aatgcataat ttttttggac aggattcatt attgcattga aaaagtttta
    39661 attttgcacc gaaacaacaa aaagaaagtt ttatgaaatt gaaagcgatc atccagatca
    39721 tcctggcttt aggcattctt gttctgattt atttcatcta tgccggtatc atgtcaccgg
    39781 tgaaattcaa caacgagatg acctcgcgta aagacatagt cattcagaaa atgaaagata
    39841 tcagaaccat tcagtctatg tatatgacct tgaatggaaa atattgctct actttcgata
    39901 gtctgataac ttttgttgaa gaaggtgaaa tgcctgttgt taaacttacc gcccgccccg
    39961 gtgattcaac ttttaccgat cccatccgcg atacaattga ttttatcagt atcaaagatt
    40021 ctctttttgg taaaaggcct gattttaagc ttgaaaatat tgcccatatc cctttttcca
    40081 atttcgaaaa tgctccgtat gacaatttcg agatgaaggt tgctgttgta aaaaaaggca
    40141 atgtccctgt caatgtcatt gaggtttttg ctgccaacaa atattatctg aaaggacttg
    40201 atctggaaaa aaataatatc gatcctgaag acggattacg gttcggttct ttaactgatc
    40261 cttctaccga cggaaactgg gaataatgaa tatacattcg gaatactgtt ttttgcctca
    40321 gggcactgta ttacctgatg aatcaataaa agtcctcgga gttcattccg aaggctttta
    40381 ttatttaatt cacagggata atgaaattct ttacgccctg cttggtagct tccgtaacgg
    40441 aataacagcc agttctctca tggctgttac gggtcgaaaa ccagaattca gaatgttgga
    40501 gatcgatcat ttattgctta tgcctcattc ttttgttgct gttcccgatg aatttctggc
    40561 cactcaaaac gaaagtcagt tgtttgaaat ggttcaggtg cttcccgaat cattccgttt
    40621 gattaaaagc gaactttctg atactggcgc ctctttgctt tctgcttttt cgtcaggcga
    40681 cctgcaggta ttgcagcaat tgatgcctaa atatccattg aaggatatga ttgcttcgtg
    40741 gttctccaaa attaaaccat atgatgctgc aaatgaagtt catctgttta ttttaccttc
    40801 ttcttttgcc atagttgttt ttgctgaagg aaagatgcag ttgattaata catttcaata
    40861 ttccgaccgc aatgattttc tttattatgt gctgggtgct gtgcaatctt gtcatgtaag
    40921 gcctgatgca acgagtctga tgctttgcgg tgagattaac ccatcgtcgc ctctggccga
    40981 agcgctggga acttattttc cgcaaatccg ctatgatgct gttgaaaata acggcgatgc
    41041 tgattcaagg cgtttgtcat ccatgttgta tcctcttttt caataatgcg tattatcagc
    41101 ggttctataa aaggcagaac atttacacct cctgaacgaa acggggtgag gcctacgact
    41161 gatatggcaa aggaagggct gttcaatatc ctgcaacatc gggtggcctt ggatggtatt
    41221 actgttattg atcttttttc aggtaccggc tctatttcct atgaatttgt ttcgcgcggc
    41281 gcagaaatgg ttttcagcgt tgaaaaagat tcggtaatga ttgattttat acaatctatc
    41341 gctaaaaaat taaatctgtc gaatataaaa cccatacgca gtgatgctta ccgatttttg
    41401 gcactcactg gggttaaagc cgatatcatt tttgctgatc caccttatgg cactcccgga
    41461 ataactgata tccctgaact cgttttcagt aaaaaacttc tcaatgatga tggaatgctc
    41521 attgtggaac acgacagtca tctgaatttc acttcacatc ctcatttcat tgaagaaaga
    41581 aaatacggga aagttcattt ttcgattttt cagtaaagca gatgttgctt tgcggtgttt
    41641 ctccatccgg cgtcgtagcg gctgtcaata aaataaatca gtaaatcagc atcatatcga
    41701 ctgtcaacaa aaaaaatctt tttgtcggca tcatagcggc tgtcaacaaa ataccagagt
    41761 cctttgtttc cagatgcatc gtagcgactg tcgcatttat aaacaagcag atcagcatcg
    41821 tagcgcgaat ccaccacgaa aatcttaata tcgggatcgt agcgcgaatc tgctgagaat
    41881 attttctggg cagatgcatt tatgattaag ctgacaaatg caattacgaa aaagaagttt
    41941 ttcatgatgc tggagttttt taaagctgaa aagcaacaat aatcctgcct tgtttatttt
    42001 tggctgacaa ttttcttcaa agattcgatt gcatatttca cttcatcgtc agttgaaaaa
    42061 caaccaaaac tgaatcttag agtgccattt gggaaggtgc caatggtctg atgggccagc
    42121 ggagaacaat gcaatccggc cctgaccatt attccgtatt ctttgtcaag tatagcagca
    42181 agatcggttg gcttcatatt tttgatgttc attgaaatca gcggtagtct gttttcactg
    42241 attgtggggc cgtaaatgct gacattatca atttgagtac actcagcgat gaaataattt
    42301 gtcagatccg tcaatgtttt acggatatta ttgatgcctt tatccaaaat atactcaatg
    42361 ccggctttga gcccgatgac accaactgca ttgatggtac cagcttcgag tcggtcagga
    42421 taaaccattg gatgttcagc cagatcggat ttacttccag taccgccatg aatcaatgtt
    42481 ctgatggcta ttccatcttt taagcaaagt ccgccgatgc ctgttggccc atatagtttt
    42541 ttatgaccag taaaagccag cagatcgata tgatcttcga ccatgtcaat aggtacaaat
    42601 ccggccgatt gtgcagcatc taccagaaat aaaatatcat gttcattgca tatggcgcct
    42661 atgtcagcaa tattctgtat ggtccccgca gcattggatc catgaatgca aatgatcatg
    42721 cgggtgtttt ttctgatgag tgatttcaga tgtttggtat cgagaaaacc ctgttcattg
    42781 caatgagcaa tgtcgaatga aatcagacct tcggattgaa ggaatgaaac agttctgaac
    42841 acggcattgt gttccatgtg gctgattata acatggtcgc cctggtttaa aatgcccttg
    42901 atggcaaaat tcagagcatg agtggcgttt gccgaaaaca tgaccctttc tgcagccgga
    42961 caattgaaca gacttgcaat cagctcacgg gtttgaagca cttcatgagc ggcttttgtt
    43021 gcaaaatgat gcccggagcg gccggggctg cctccgtatt cggatatata agccgttatt
    43081 gatgaaatta cgccagccgg tttcggccag gatgtagctg aattgtcgag ataaatgctc
    43141 attttcaaat attgaattcg tacaaattta gtgaattctg gatgattgag cattaatttg
    43201 aaaaacaggt gttcaattgc aaaaaatagt tttttaattg atataaatca tcaaaagcga
    43261 gagtcttatt tagaatagat ataaatttcc ttaaatcctt agaaaaaaag gtataatcat
    43321 tatttcagaa ctattttgtt tgctaatttt ggccgtcctt acaaataaga aagtcctaat
    43381 tcaaaacata tgaaagaaaa aatcaccagt attattgagt cgttttctaa cgatgccgca
    43441 cggttgatgg atatcctttc cgaagtacag tcagagtttg gctgtgtttc gtgtgaggcg
    43501 gtatctgtta tagcatctgc gcttcagatt tctgaggccg atgtagagca gacccgttca
    43561 ttctatcatt tctacacgaa agacccggta ggcaaatacg ccatttatct caacaacgca
    43621 gttgttgcat gcatgatggg tatgggagaa attgtcgatg agtttgagaa acagctcggc
    43681 attaaaatgg gtcagcgcac agccgacaat atggccagtc ttcactttac ggcttgtatc
    43741 ggaatgagcg atcaggaacc cgctgccatt atcaatgatg ttgttttcac ttcgcttaca
    43801 cgcgataaag tgaaagccat tgttgcagac atgaaagccg gaaaatgcgt aaaagacatg
    43861 gttaaagaag tcggagatgg caataatgcc aatcctttgg ttcattccat ggtaaaaaac
    43921 aatattaaaa aagcaggtcc tgttgttctt gatgcatatg agtccggagc cggccttgcc
    43981 aaagctcttg gcacaacatc gcaggccgtt atcggagaag ttcaggcatc gggtcttcgt
    44041 ggccgaggtg gtgctggttt cccgaccgga atgaaatgga tgttctgtgc aaaggaaagt
    44101 ggcgataaat atgtgctttg caatgccgat gaaggcgaac ctggaacatt caaagaccgc
    44161 gttcttctga ccgaacgtgc tgctatggtt ttcgaaggaa tggccattgg tggatatgct
    44221 atcggagcaa aggaaggtat tttgtacatc cgtcgtgaat acaaatatct gcaggcattg
    44281 ctcgaggaca cgctgaaaaa tatgcgtgaa aaaaatctcc ttggcaaaaa cattatggga
    44341 aaaggatttg attttgatat ccgggttcag atcggtgccg gtgcatatgt atgcggcgaa
    44401 gaatcagccc tgattgaatc agctgaagga aaacgcggcg aaccccgcaa ccgtccacct
    44461 ttccctgcac aaaaaggata taaaaacaaa ccgtcaattg taaacaacgt tgaaactttc
    44521 tgcgctgttt cgcgcattct cgacaaaggt ggcgcatggc acaaaggttt tggcactgac
    44581 gagtcggccg gtgtgaaagt attgagcatt tcaggcgatt gcaagaaccc gggtgtatat
    44641 gaaatcgagt ggggcatgac cattcagcaa atgcttgata tggttggtgc aacggatgtt
    44701 caggccgttc aggtggctgg tccttcgggt gtatgtctta atccggagca gtttggacgt
    44761 aaaatcgccc ttgaagatct tgcaaccgga ggttctatga ttgtaatcgg aaagaaccgc
    44821 gatttgctca aagacgttgt cttgaatttc atggagtttt ttgctgatga atcctgtggt
    44881 tcatgtgtaa cctgccgttc attcaacatg atccttcgca acgcaattaa aaaggttgtt
    44941 gaaggcaatg cgattaaaaa ggatgttgaa gacatgctgg catggagtga aatcattcgt
    45001 aaaaccaccc gttgcgggtt gggacaaaca tcggcaaacc cgatatcaac caccatcaac
    45061 aacttcagac atttgtatga tgcaagagtg aaagatgttg agtttgcaag tctttttgac
    45121 atgagcaaag cggttgcgga atcatgtgaa tatgtaaatc gcaaacctgt tgttcacgaa
    45181 taatccaatc agtaaaaaac aaaaaagaaa aataatatga gtgaaacagt aaaattcacg
    45241 attgacggca aagaatgtac cggccagaaa ggtcagtata ttgttgatgc agctgctgac
    45301 aacggggtat atattcctgt gctttgccac atgagagacg ttgttcctgc cggctcatgc
    45361 cgtgtttgta cggtaaaggt aaacggacgc aatatggcgg cctgtactac tccggttgaa
    45421 gatggaatgg ttgtagaaaa tgttaccgaa gagcttactg atatccgtaa agccattgta
    45481 gaaatgcttt ttgttgaagg aaatcacttt tgtccttcat gcgaaaagag tggcaattgc
    45541 gaccttcagg cgttgggata taaatatcag atgatggttc cgcgttttcc gtatgcattt
    45601 gagcgtagaa atattgaagc tactgcaccg aaaatttatc tcgaacgcaa ccgttgcatt
    45661 ttttgcaagc gttgcatccg tatgattaaa gataaggatg gcaagagcat ttttgcattc
    45721 aaaggcagag gtcagcacct tgaaatcacc atggatctta aattagctgc caacatgacc
    45781 gatgaaatgg ctcagaaagc catggatatc tgcccggttg gttgtattct taaaaaagaa
    45841 gtcggattca ttgatccgat tggccagcgc aagtatgaca aaaatgtcat tggttcggat
    45901 attgaaaaaa taaccaaata aggaggacta aaaatgagta aaccaattgt agcaacaact
    45961 tccttagccg gatgcttcgg atgtcatatg tcattactcg atattgacga aagaattctc
    46021 aaactcattg aactcgttga attcaacaag tcccctattg acgatatcaa aacattcacc
    46081 aaacagtgcg atatcggtat cattgaaggt ggctgctgca acagcgaaaa cgttcatgta
    46141 ctgaaagaat tccgcaagca ttgcaaaatt ctgatctcat tgggcgaatg tgcaatcatg
    46201 ggtggtctgc ccgctttgcg taatggtatc cctgtgaaag agtgtctcga agaagcttat
    46261 ctgaatggcc caaccgtagg attgaacgct gaaaaaatcc tgcccaacga tgatgaactt
    46321 ccgatgattc ttgacaaagt atatccttgc cacgaaattg taaagatcga ttatttcctt
    46381 ccgggatgcc ctccccgcgc tgaccttatc tgggaagccg ttgcagccct ggtaaccgga
    46441 aatccgatga atcttcctta cgaagtggtg aaatttgatt aatcaaaaaa cgaaaaagac
    46501 aatggcacaa aaagtaacaa tagaacctgt aacccgcgtt gaaggccacg gtaaagtgac
    46561 cattcagctc gatgataata tgaacgtaac ccagtcgcgt cttcatattg ttgaattccg
    46621 cggatttgaa cgttttgttc agggccgtcc ctactgggaa gctcctgttc tcgttcagcg
    46681 cctttgcggt atttgccccg taagtcatca tcttgctgct gcaaaagctc ttgatgtaat
    46741 cgttggcgcc ggcaccggag aaggacttac tcctaccggt gaaaaaatgc gtcgtctgat
    46801 gcattacgga caaatgctcc agtcgcatgc tttgcatttt ttccaccttt cttctcccga
    46861 ttttttgttg ggatacgatg ctgatccggc tatccgaaat gtcatcggcg tagcactcaa
    46921 tttcccggac cttgctgttc agggtgtgat gatgcgtaaa tacggacagg aagtgattga
    46981 acgtacagcc ggtaagaaaa ttcatggaac cggagctata cctggtggca tcaacaaatg
    47041 tctgagcgct gaagaaaaag cttttctgct caatggttct gatatgatga atatcgacaa
    47101 gatgatctca tgggccgatg cagctgttca attcttcaaa gaatactaca tgaagaataa
    47161 ggccatgatt gacggatttg catatttccc gtcaaatcac ctgagtcttg ttcgcaaaga
    47221 tggcgccctt gatttctatc atggcgtaat tcgcgcggtt gatgctgatg gaaaccgtgt
    47281 tcttgacgat gttgattatc aggaatacaa caaatatata gaggaagaag tacgctcatg
    47341 gagctacatg aaattcccgt atatgaaaca cctgggtaaa gaaaaaggct ggtaccgcgt
    47401 tggtccgctt gcccgtctca atacctgtga ttttattcct tcaccattgg ctcagaaaga
    47461 atttgaattc ttcaaatcgc agacaaacgg caaacccaac aattactgta tgcatacgca
    47521 ttgggcacgt ttgatcgaat tgctgcatgc tgctgaagtg attaaagatc tgctcaacga
    47581 tcctgatatc atgaaaaatg atctcgtgac caaaggaacc aaacagagcg aaggcgttgg
    47641 cctgatcgaa gctccgcgcg gaaccctgtt ccatcattac agaatcaatg agcatgatca
    47701 gattaccatg gccaacctca tcgtttcgac aaccaataac aacgagccga tgaaccagtc
    47761 ggtgaatttt gttgctcgcc gcatgatgac cggtcagaag gaaatcaccg agccgatgaa
    47821 gaaccaggtc gaaatggcca tccgcgccta tgatccatgt ctgagctgcg caacccatgc
    47881 gcttggtcag atgccgctcg acatcaaact gattgattca aaaggcgaaa tccttgatcg
    47941 ttacaaatca taagcatttt gcttttttat acaaaaaagt ctgtcggttt ccggcagact
    48001 ttttttgtac agggcataaa cgtttgatat agcttgtaat aaaagaaatt ttgtatgtac
    48061 ctttgccgta tgaaaaagac attgatatac ggatacggta atccgggccg catggatgac
    48121 ggaatcggca aacgatttgt tgatttgatt gacaactgga ttgcagaaga aaaaattgaa
    48181 aatattttca cggattgtaa ttatcagttg aatattgaag atgcgtccat tatatctgaa
    48241 tacgatgccg tagtctttgt cgatgccagt attgtggagg atgttgagaa tttccgtctc
    48301 gaaaaagtgg agcccaacga tgctaccatc gaatttacca tgcatgcggt ttccacagca
    48361 tttgttgttg atttatgccg taaaatgtac aacaagattc ctgaagcatg ggtgttgcat
    48421 gtgcgcgcct atgagttcga tttcaaagag gaaatgacac ccaaagcggt tgaaaacatg
    48481 ctcgatgcgt ttgtgtttct gaaagatttt ctaatgaaaa tggcgtaatc cgtcacggaa
    48541 ttattgagca actgaaattt gaatcactta aaaatattat tactttcagg ttagttgaat
    48601 ttttctattg tagtcgctaa agtgttattt agagctgatt tatatggatt ataattttgc
    48661 tccgatgttg ttataacccg ccataagtgg gtaaaaaatg gcacttctgg cgagttataa
    48721 atgtataatt ggccaaatgt ggaaaaaatt gtatatttgt ggcgacttat aatgatccgt
    48781 tatgagaaca attgtcggca gagactttga aaaggatgag cttgaaagct tttaccaatc
    48841 ggatgtttcg gaatttgtgg ctgtgtatgg ccgcaggaga gtcggaaaga cttttcttat
    48901 ccgggagttt ttccgggacc ggtttgactt ttatctgaca gggcttgcaa atggcggttt
    48961 cagtgagcaa atggccaatt tcaatcttgc tttgcgtgag gctggactga aagacctgcc
    49021 attggcaaaa aactggattt atgcatttca gcaattgcgc atgctgattg aaaaatcaag
    49081 gaagaaaaag aaagtgattt ttctggatga aatgccctgg ctcgatacac cgaaatcgaa
    49141 gttcatgagc gcattggagc atttctggaa ttcatgggct tcgggccgga atgatattct
    49201 gctgattgtc tgcggatctt ccaccacatg gatgaccgat aaactgctga acaacaaagg
    49261 tggcctgcac aaccgtatta cgcatcagtt gcggctggag ccgtttaccc tgaaggaatg
    49321 tgctgcatac gtcaaatcgc tgaaattcac ctggagccag catcaggtgg ctgaatgcta
    49381 tatggcgctt ggtggtattc cgtattactg gagtttgcta aaaaaaggaa agagcgttga
    49441 ccagaacatt gaccacctgt tttttggagc caatgcccgc ttaaaagacg agtacaaaaa
    49501 cctgtatgag gccttgttta aaaactcaga caagtatatg caggttgtgg aggcattaag
    49561 caaaaagagc agtggcctga cacggcagga aattgttgaa agcattgaag aaagcagtgg
    49621 cggaggattg acaaaaatac tggaagatct ggaaaactgt ggctttatcc gctcctttca
    49681 ggcctataac aagaaaagcc gtgaccggat ttttcagctt tgcgattttt acaccttgtt
    49741 ttatttcagg tttgtgcgtc gcgccggaag ggataaaaaa tactggaaga gcatgatgga
    49801 caccacgcag catcgtgcct gggccggtta tgcatttgag cagctttgca ccatgcacgt
    49861 tgatcagctg aaacacgctc tcgggatttc cggcgtgtat tgcaatgtct attcctggcg
    49921 cagcccggcg ggcactgaca aaggtgcgca gatagatctg attatcgacc ggaacgacaa
    49981 tgtgattaat ttgtgcgaga tgaagttttc cgcgcatcct tttgtgatta ataaaaagta
    50041 tgcggatgaa ataaagaata aatctggcac tttcaggagt gtaacaaata caaaaaaatc
    50101 ggtatttctc acctttgtat catcatccgg attggctcag aatgttcatt ccggaatagt
    50161 tcaatgtgag attgaattaa aggatttgtt tgctgtttaa agtttataac ccgccataac
    50221 taggtttttt gacccacttc tggcgagtta taaaatattt attttcaata aaagtttgtt
    50281 tttgcaggta catcagagcc ctaaaaatac cattcctatt ccgcaaagca gaataataaa
    50341 accagcaatt gcatgagcgt agcgtcctgc ttttttgagg ttcagaaagt gaaatcctct
    50401 gttggccagc accaccaagg taatcatggt tccgatggta ataatggcga ataaagcggt
    50461 gacagtgatc agcaacaagt aattgctttc tgcagcgggg tacatcacaa gcagaatcaa
    50521 tggttcgcag ggaccaaaga taaaaatggt gaacagaatc cacggcgtta tgttgcgttt
    50581 ttttttagta tgtgcgtgcc catgagttga atcgagcgaa tgaatgtgtt cgtgctgttc
    50641 tccattttca tgcaggtgca gatgtgtgtg tggtttgttg cggatagcac ggatggtgcc
    50701 ccagatgaag tatccgagtc cgaaagccat gagaagtaca gacacaatgc tgccacgctg
    50761 gccttcccag agttcgatat gactgatggc aatacccgtt gcaacaccga ttattcctaa
    50821 caacacagag cttcccacat gaccaagtcc gcacaggaaa gtaaagacaa cagttctttt
    50881 aagcgaccag ttgcgggctt tggatatggc gatgaaagga agataatgat cggggccggc
    50941 cagtgtatgt acaatggcga tggatgcggt agttgtaagc agaacacttg tttcagctgt
    51001 catatttttg tacttacttt attttgaaat gaatgccgtt accgtcgagc atattgtatt
    51061 caaatccgcc acaggtgaga cataccgctt tgttttcagt attatcggct gttcgggtgc
    51121 tcatgatgct ttcgccgcag gtcgcacaga taatgctgtc gtgtattttg gcatattgag
    51181 gaatatcagt ctgcacatgc tgaacattga acagcgattc gaaaggaatc gatagtgtgc
    51241 caaaagcacg attcagtgag gatttccggt attcagaaac caataaggag tcgtggtttt
    51301 ggtttgcaac aacctgctga tacaaatttt gaaaatcagg aaatgcatct cggatgactt
    51361 gttgtgaatc ggctttggat gaaatccgga ttcctttgcc atcgcggtga gtgagtgtga
    51421 atgcggtttt accgaggtct ttaaagatta aagcattgtt gccgaaggtg catccggtaa
    51481 caaattgcac gccgtcggaa aagcagttat tggtttcggt aatggccagc agatcttcca
    51541 taccgtccga ttcggcttcg agcagattca tggcttttgc agcagccata atgcccatgg
    51601 ccagtcccgg acaaaaatgc ccgtgcatct gagcggcttt aatcatgagt gattcgtacc
    51661 tgtttgaata aatttcgttg cgtatatcga tgcgcggact gctttttagt ttggcctgat
    51721 gcagcggatt attgtcggct tcgtaggcaa aaagagaagt atcacaattt ttaatatcaa
    51781 gtaccggcgt accattgagc gcatcaagtc ctttgaccag caacatgttt tcgtttcgtt
    51841 caatcagacg aacgctggtg acaccaatca ggttaggcct gcgcgggctg cggtttccaa
    51901 atacaccccg tgaaatatca gggcggtttt tcgtattcga aatcaaggtt gattccgatt
    51961 tgtggaaata aaaaacaata tccagaaatt cgttttcctc aatacctgtc agtgcttcca
    52021 ggtattcgtc aaatatctca atggtactgg gttgattttt gattttgtga taatcctctg
    52081 aattgtcaat cagattatgt acgattccga tgggtgtaaa ggattgtatt ttcatagagt
    52141 tttttatttt tcgattggtt cgagcgggat gacatgaaaa gaattttctt cttgcaggat
    52201 ccttactttc acatcatata atttttcaag gtttttttct gtaatgattt catttccgcc
    52261 tgaggcaaag acttcaccat cgcgaagcat gagaatacgg tcagcaaacc ggatggctgt
    52321 gtttatatcg tggagtgcta caatgattgt caatcctttt gctgaaagtg ttttcagcag
    52381 cttcattatt tcgacggtat gcttaatgtc gagatgcgca gtaggttcat caagtaaaat
    52441 aatgtcgggt tgctgtgcca atgctctgcc aataaaaaca atctgttgct ggccgccact
    52501 cagttcattg atggatgaca ttgaaatact ttcgagctgc aattgttcaa taatctgcga
    52561 tgtaatggcc agatcttttg ttgatgggcg ccagttgatg tagggtttac gaccaagcat
    52621 aatggtgtcg aatacgctca ggttgggcaa aacggatttg ttttgaggaa cataggccag
    52681 tttttcagcc agtttgtttt ttggatacaa ccgaatattg gcatttgata tttttatctg
    52741 accgctttgt ggttgcagaa tgccgttgat gcatttaatc agggtgcttt tgcccgaacc
    52801 gttggggccc gcaaggacga taaattcgcc ctgacggatg tcgatactca ggtttttcag
    52861 cacttgattt ttgctgtaac taaaactgat attgctgatt gaaatgctca tgtttacgaa
    52921 tagtttacag gttttttcat gccttttatc agaacaaaga gaaataacgg agctcctaaa
    52981 aaagaagtca ggatgccgac cggcataacc accggtgaaa aaatgcttcg cgccagggta
    53041 tcggatgcca gtagaaacaa tccgccaaaa agggctgaaa caggaatcag atattgttcg
    53101 ttgtcgccgg ttattcgccg gatgatatgt ggaacaacca gtccgacaaa agcaatgatc
    53161 ccataaaacg atactacaac ggcagttaca agcgatgcaa tgatcattcc tctcatccgc
    53221 acttttttta cacgaacacc aatgctttgt gcgtattcat cgccggtttg aagcgctttg
    53281 taattccatg actggagtat aaaatagatg gttgcaggaa ttacaataaa ggataaaatc
    53341 agcagattgt catagtgggc tctgcttaaa tcgccgaaag tccagaaaac aatggacgat
    53401 aattgaacgg tatcggcaaa atattgcatg gccgaaatgc cggcaccaaa taatgagctg
    53461 atgatgatac cagccaatat gattatttca ggtgtggctc ctttcagacg cgataaggca
    53521 agaatgagta caactccagc cagactgcaa atgaatgccg acagagaaat taaatatgga
    53581 tgaattgctg attcagagat gcctgcaggc agtgcggtgc tattgaaaaa aacgatgcat
    53641 aaagctgcac caaatgcagc ggcgcctgaa atacccaatg taaacggcga tgccaacgga
    53701 tttcgaagca gactttgaat ggctgctccc gaaacggcaa gcgctgttcc ggccagcata
    53761 gcgccaagaa tgcggggaat acgaatttga aataaaatct gttcagtaaa agcatcggag
    53821 tttccggtaa taagttggaa aacgtcgttc aattgataat cggcggaacc agaaagtagt
    53881 gctataatga ttgttgccac gtataaaata caaatactgg caatgaccaa cagttttttt
    53941 ctgctgtttt tgcggtattc atttataaca gagctgtttt tcatcagtgg acaggcgtta
    54001 ttttgcgcaa ccctaacgca gatttcattt gtgatagcga aacaggtttg ttgaagaaca
    54061 aggtcaatac ttcactggct tttgtttcaa tgtcaatgtc ttcaaactgt tgcggataaa
    54121 ttattttgcc tgcaaaccat gcattcagca gcacaaattc gtaattggtt gcataattat
    54181 tatatggcag caggcagtac aactgattac tccggatggc atccaatgac tttttcaatg
    54241 cgggttgatt gtttatatcc tgcagtgcaa gcgaatatcc ggattcatca atgaaaatta
    54301 tatcggggtt ccaaagcagc aattgttcaa tgtcaatatt ggtgcccttc acatgcgaag
    54361 aaacgatatt ttgttttcct gaaactacat tattggcatt gacaaaaaga aaaggagcaa
    54421 atgcaccgtg tgttgatgca atgccgtgtg caccgctgta ggccacgcct ccgacatata
    54481 caccgggctt gtcttctggt tttatcgacg aagttcttgt gtttaattct tctatcatgg
    54541 aagaaatgcc tttaatcaga gaatcggcgc ggttttcttt gtgcagaatt tttccgataa
    54601 gactgaatga atcaaacaaa ccgttggaat tcacttgcag atctgggcaa tcgatggcaa
    54661 caacaggaat gccggttttt tgctgaagtg catcagcttc gccaacagta gagtacgaaa
    54721 taaaaattac atcgggttgt gctgcaacaa tcagttccgg gtcgccaccc atttgtggtc
    54781 cgacagaagg cagcttcatc agttcgggaa acgcaagcag atagggacgt gagctgcgtt
    54841 tttcattttc ttcgacgccg gcaatcatat ccgctgcatc catatatacc agcaggcgta
    54901 aagctccggc acgcagggca acaactttat caatgttggc cggaactgta actttgcggc
    54961 ccagcatgtc ggtaacaaca atatccggat cactctgttc agcgcgaccc ggattattgc
    55021 aacatatgaa agaaaaaaca ataatcaata atacagatga atgcaacaag cgataatgca
    55081 tcatgtctga tcagagttta agtgtgatac ggccattaat caaacgtccg ggactcagat
    55141 aaccattgac ttccatttgc tggttatcca taatatcctg aatatcgagt gagagcgaga
    55201 ggttttcgtt gatttttctt gaaatcatca ggtcaaaagt caggtagctg tcaagtgtaa
    55261 tattgttttc atcgtcggca aactgcgcac ttttatataa tgcccgaccc gacacgttaa
    55321 tgattttatt ctgccagaac gcagagaaag atgcgttgtg agccggaaca tatttcagtg
    55381 atttattctc aaggtctggt ctttcagtga atccgacaat ttttgaatct gagaatgtat
    55441 agcttccgct gaataaaata tttttggtca gggtataagt tgcttctgct tcaaaaccct
    55501 tgatttcgac gctggttacg ttttcgcggc ggtagatggg acgtccgtaa atactgtcgg
    55561 aagtagctac ataatacaga aaatcatttc cctgcatgta atatgcgcta agcgcaattt
    55621 tcagattttt tgcaggaaag atatcgaatc ccgcttcgaa gttttccatg gtttcgggct
    55681 tcagttccgg attggcatat tttggtccga cccacatcca gccggttctg attaggtcgt
    55741 cgaggattga cgccctgaat ccatgggagt aggagacaaa tgcgctgagt tgtttaatga
    55801 aattgaagcg taaaccggct ctgggagaga ctttacgcca ggtgttatct ctgagttccg
    55861 gaatggtatt ccatgggttg gtattttcgt gcagtccatt cacaaacgaa acctgatcga
    55921 aacgaatacc agccagtagt cgtatttttt tcttcagaaa ggattgttcg tcctgaaggt
    55981 agcaagccaa ggtattgatg ttgccttcat tgtatatggt gtcgtagggt gcggtttgat
    56041 agcaatcgcc tccctggatt ccacccgatt tatactcgaa gccggcagtg agcgtattgt
    56101 tttgattcag aatacggttg acattggcca gcatgcccat atccgtgcgt tttgaattta
    56161 catcgtagcg tgtatatacg ccggttccgt ttaatttttc attcacgtca taataatgct
    56221 ccaactgata ataagtattg aaagtgtagg atgtgttttt atttccaccg cgcagcattc
    56281 cgcgcagcag attggtgttg aaattccggt agcaaccttc gggcgtgtga atctgataac
    56341 cttcgccacg ctgatcgcgg aacacatcgt attgcagctc ccaattcaac catcggctgt
    56401 tgtcaaaacc agttcttgct gaaatgccca tggcttcaag cgaccgcgca atatcgtatt
    56461 gtgttctgtt ggtgtccggc acactattat agccgtcgct ttgctgataa aattgagaca
    56521 gaactgcgta gtaacctttt ggattgcgga ttcgcacgtt caaatcctga cgaaaagtat
    56581 tgtatgttcc gtatcctgcg ctgcagatgg cttcggaagg tttttccgct ttttttgtaa
    56641 tgatattgat gacgccaccc atggcattat tgccataaag cgacgatccg gggcctttaa
    56701 atatttcaat tctttcaatg tcgttctgat tgatccggtt ccagtttaca ccgccttcat
    56761 cgctggtatt taccggaacg ttattgacaa gaaccagggt gcgaccttgt tcgtcgcccg
    56821 aaagtccgcg gagcgttacc atggggcgct gggtatagag gccggtattg cggtttacgt
    56881 tcacgcccgg aataaaacgg agcaggtcat cggtttgcag ggctgctgtc tgattcagca
    56941 aaacgctgtc aataatatta atgcgggcag gaatttccag cggattacgc tcggttttgg
    57001 tggcggtgat aatcatttcc ttaatggaaa tagtatcgct gatttcgttt gtttgagcca
    57061 gcgctgtgga tgtaagcatc agaataaata tcgggataaa aaaagtcagg cgtcctgtca
    57121 tttgtctttg ttgtttgatg tctgttgcga tatttcgttc tttattgtga ctttgattag
    57181 ctgaattttt gaatttaatc atgtcactaa aatacaaaaa cgtgttacca tggttgcaaa
    57241 aaaaattatt cggtggtggt tattacaagt tttccgtgtt taacgccttt gatggcaatg
    57301 agttggtcgg ccagttcttt cagtttacct gatttgccca tcaccgcaat ggtttcgagg
    57361 caattgtgat gatcgatgtg cacatgcatg actgataaaa taatatcgtg ataatcgtgc
    57421 tgaatggctg tggatttgtt ctgcagctcg cgtttgtgat gatcgtacac cagaatgatg
    57481 ctgcctgcta caatcttgtt gccatcggtt ttttcctcga ccagataatt ggatataagg
    57541 taacggatgg cctgcgagcg attcggaaat ttgtgtttat tcacaaggtg atccagatcc
    57601 gaaagaatct gttcatcgag tgacacgccg aatcgtttta ctttcatgtg ttaccttttt
    57661 tcgaaaagat ggcacaaaag tataaaaaac tacagcagtt ccgctgtgtg tctgtaattt
    57721 ataatcagat taaataagcg ttatggtcta ttgtgagatt attttttctt ttgttgttat
    57781 actaaatgga aatataaaac agtcaatcga agcaatttca ttgaaggttt agttggctga
    57841 tgccagaggt ttaactgaca aacagacaaa tatcgtctat ctgtagaaaa acgaatacct
    57901 gccatacgat ccgctggctg gcggatcgca cattaacaag caatgtcaga agactcagct
    57961 ctttatatgc gactcctccc gagtcgtggt tttgttttta tgggctacag atatatgact
    58021 cctccggagt cttccaaaaa taattgaacc gatttataaa ccactttggt attatacggg
    58081 caaagcaagt tgttacattt tcacgggatt ttctgatgtg atgaaataat caggtattgc
    58141 gatcaaagaa aaaatttcgt taccgtcact ttggaacaaa aaacagcaaa aaatactgtc
    58201 actttagaac aaaaaatgtg aaaatatacc gtcactttga aacaaaacat gctaaaaaat
    58261 accgtcactt tgaaacaaaa tatgtaaaaa aataccgtca ctttgaaaca aaaatcaata
    58321 aaaagtaccg tcactttgaa acaaaagatg tatatttgcg ttgaaaatca aaaagatgca
    58381 tatgtttgac aggaaggcca ttcagaacat gaatcggtgg atgaacaaaa agaaccggaa
    58441 gccactggtt ttgagaggag cccggcaagt tggcaagacc actcttgtga aggtatttgc
    58501 aaaacaattt gggacatttc tgcatttgaa cctggaggac aagcgcgcag cggaattatt
    58561 ttcggtgagc agtggcatgg aagatctgct ggcagccatt catttatatt gtaatattcc
    58621 agccggaaaa ggcaaaacgc tgttgtttat tgatgagatt cagaattcgc ctgaagccgt
    58681 agcaagactt cggttctttt acgaagagat gccacagatt tatgtaattg ctgccggttc
    58741 attgctcgaa agcatgttga ataaaagtat ccggtttccg gtaggccgtg ttgaatttct
    58801 tgccatcagg ccctgttcat tttcggaatt tctgaatgca acaggtgaaa aaatgctggc
    58861 tgaaagcatt gataaaatga gcatgccagt cgctctacac gagaaagcca gccacttgtt
    58921 caaccgatat acactggtag gaggcatgcc tgaaataatt gctgaatatt ccgaaagcaa
    58981 ggatattgta tccctttctg gccttttcga ttcattactc acatcataca aagatgatgt
    59041 cgtgaaatac gcccgtaatg aaacgatgga gcatgtcatt ggtcttattt tgcaggaagg
    59101 atggaaatat gctgctcaga ccatttcgat gaccggattt gcgggatcgg cttacaaagc
    59161 ccgtgagatg ggtgaagctt ttcgtactct tgaaaaaacc atgttgcttg agttgtgtta
    59221 tccaacttcg ggtgccattt tgccgataca gcccgaaaca aagcgacagc ccaaacttct
    59281 atggcttgac accggtattg taaactatgt tgcgggcatt caatcagagg ttttcggatc
    59341 tcccgatatt ttggatgtgt ggcgcggaag ggttgccgag caggttgcag ctcaggaatt
    59401 gcttacactg gatgaccggg tgacctatcg caggagcttc tggatgcggg acaaaaaagg
    59461 atcggatgct gaggtggatt ttattgttca gaatggcagc agcataattc cggttgaagt
    59521 aaaatcggca cataatgcgc atctgaaatc attgcacagc tttatggata ttgcacctca
    59581 caatatagcc gttcgtatct ggtcaaagcc ctttgatgta tcgatggtga aaacaggaaa
    59641 aggcaaggaa ttccgcctga tcagcctgcc attttattat atctcgaaac tgcatgaatg
    59701 gattgatagg gaatgagtgt gtataacttc acgcccatgc tctgcgcgtt atacccaatg
    59761 agttgagccc tatgccttct tcagccactc ataccacttg tccatgcctt cgccggtgcg
    59821 ggccgagact tcgaaaaact gcaggtgatg attgattttc agcgcatttt ccttcacttt
    59881 ttctacgctg aaatcaacgt agggcaggag atcggtttta ttgataatgc agatattgga
    59941 agtgctgaac atattgggat atttcagcgg tttgtcgtcg ccttcggtca ctgaaatgat
    60001 gactacacgc gctttttcgc ccaggtcgaa caatgacgga caaaccagat tgcccacgtt
    60061 ttcgatgatg agcatggaat tgtcggctac gttgagttct tttacagctt tgctaatcat
    60121 ttcggaatcg aggtggcagc cgttgccggt gttgatctga attacaggag cgccggtagc
    60181 attgatgcgg tcggcatcgt tcatggtctg ctggtcgcct tcgatgacat aaaaattcag
    60241 gtcgtttttc agcgccgaaa tggttttttc gagcagggtt gtcttgccgg aaccgggcga
    60301 actcactaga tttaaagcat taatattttt tgcatcgaaa tagccgcggt tgcgctcggc
    60361 cagcgactgg tttttatcga gaatattgtg ggccagcaca aattcctttc cgtgcggatg
    60421 tgcatggtcg tggtcatgtc ccgcatgctc atgggtgtgg tcgtggctgt gcgcgtgacc
    60481 atcgtggctg tgtgaatgaa tatagttgtg ttcgtgctca tgatcgtgat gctcatgatc
    60541 gtgctcatga ttgtgtgcat gcgcatactg atgctcgtgc gggtgcacat gtgcatgttc
    60601 atgcgagtgt tcttcgccgg gtttatgata ggtcactttc tgattcgggt cgctgcagcc
    60661 gcaagtatcg cacataatag tgtagtttaa ttcagtgcaa aaatacgaaa atcatcatta
    60721 tcaaatactg tattgtatga actttgcaaa tagtatgtat caggccttga atgtttgttt
    60781 acaacagcaa agcggcaatg attgtttttt acgtttgcga tatatgttga atcgtttaat
    60841 tggtaattat tacaattaaa tcctttgcgg tgagcaagat acatcagctg tggattgccg
    60901 tctccattaa cgagtatcag gtctttttgc gaagagaatt tatcaagtat ggattcaata
    60961 gtcagtttgt atttttcaga gtccttgatg aagaaatcat gttgttgatt ggcgatgctt
    61021 tcggttattc cgagcagcag aacactgaac ataatccatc gcttattgat ggaagcaatt
    61081 gcgtagccgg ccagcagagc cattaccggt acaaacggaa tgatatagta attgtgatga
    61141 tagaagaaaa atcccgattt gaatatgtaa atcagaaaca ccaggaaaag caatgtgaaa
    61201 ccaatgaata agggagtgtt ttttttgtaa atcatcagaa acaatcctgc ggcgaatacc
    61261 ccaaacatga taaaacctga aaatgcatca aaatagaaat tgtcaagtgt ttttccaagg
    61321 ttatcggata gttcttttat ccctttaagc atgggttttc cgatattgta ccagttccca
    61381 tattcactgg caaggtaggg attccatata aaataccacc aatacgccag gattaatggt
    61441 accgctgtta caacagaaat ggttattttg tttttgagtt caaattttcg gcttaataca
    61501 aatggcaaaa gaacgaccag atatatgcct gcaggaattt ttgataaaag ggcgagagag
    61561 ctgaaaacga catataaaaa cagaagcgga tatttatgat tgtgcagaaa tttcaatccg
    61621 caatatagag cgataaacat cagcgaaatg caataggtat cgggcatcat ttttcttgaa
    61681 aaggagaacc agatggacgc ggataaaatt attgttgcat aaaacgcggt cctttcactg
    61741 atgcctgcag tttttgcaca tttataaaag aaaaacaaac ccagcgaaga aacaaccaga
    61801 ttaatcagtc ttccatacca gtgtgtatat ccaaataatt cagaaaacag gtagtggagg
    61861 taattcattg aaggaaactc catggcaata atatctgatc cgccattgaa atcatcaatt
    61921 cgtggatata aaacatttgc atccacttca acgaaattgc gtgcaaccat caagccggta
    61981 acctgcctcc agttgtggct gatttccaat ggaggattgg tgattccgat tagccggatc
    62041 agaaaaaaaa caaatatcca gaattcagta actgtctaaa ttttattttc taattctatt
    62101 aagcataagc ttaatcattg a
//
LOCUS       DAOPKE010000008        56654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_582_length_56654_cov_12.222283, whole genome shotgun sequence.
ACCESSION   DAOPKE010000008 DAOPKE010000000
VERSION     DAOPKE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_582_length_56654_cov_12.222283"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKY63_02120"
     CDS             <1..1059
                     /locus_tag="PKY63_02120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPE99439.1"
                     /translation="CHIGRMIIYNVADVNDYKKSPQLPLHKSDKPFYFFEKLLPELDT
                     TVFPKRFITEDEKITNWESLFLETKTTSFLIIKNDTIKYEKYFLGRDENSIMTSFSAN
                     KSFISALVGIAVAEGKITSIKDPITKYLTDFKNPGFEKITIEHLLNMESGIRFRENYF
                     NPFAEIGKYYYGNNLKKYVPNLKVEKDPGTEFQYRSVNTLLLGMIVEKASGVPIGQYY
                     QDKLWTPLGMECDATLNTDSKSGNQVKCYTGLNARSIDYAKFGRLYLNKGQWNGQQII
                     PEEWVMATITPIVKERSSEYYKYQWRIDNAGNFWAAGLLGQYIFVNPRTNVIIVRTGK
                     KNGSVNWPTLMAHISKKI"
     gene            1061..1564
                     /locus_tag="PKY63_02125"
     CDS             1061..1564
                     /locus_tag="PKY63_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPE99440.1"
                     /translation="MPKIGVLSDTHGSLPDSVGEFFAGCDEIWHAGDVGNDAVLKQLA
                     SIASVRVVYGNIDGQDVRGVARDHTFFISGGKRVLMKHIGGRPGAYTPETREQIAALK
                     PDIFVCGHSHILLIQFNQKNNLLHINPGAAGSSGFHKMMTMLRFDISDGNILNMEVWE
                     KPRKALI"
     gene            complement(1557..2015)
                     /locus_tag="PKY63_02130"
     CDS             complement(1557..2015)
                     /locus_tag="PKY63_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPE99441.1"
                     /translation="MIQRIQSLYLLGGALAILAMIFIPFSRVIADSAAGYLYLHECKG
                     DVLVAGVYNPLLLLFAGTITAVSFLISIFLFKNRIRQMRFNAFVFILNCLLIGAMFIV
                     PERLADAVHGHAYYKEIGIIMPLISMVLLVFANKAIKKDEMKVRAADRLR"
     gene            complement(2047..2763)
                     /locus_tag="PKY63_02135"
     CDS             complement(2047..2763)
                     /locus_tag="PKY63_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HPE99442.1"
                     /translation="MISAVRCIILHTVKYGDNGLIIKSLTAKGIRTFFVASYLKNNKN
                     KYPFLSYPLAITECQYISGTPGKLPLVKSLSLDYVPRYGAFDFRRQAISTFMAECAVK
                     YIREESMTPELFIYMEQCIRFVHESEYLPAAYPVQFGFGLASLLGFGIDSTANAKEKI
                     IFTLDKGFVPGTTQAALNQTNSEFLSEIISAEINIPFETRIPSRSLKEIFQVMLQYFR
                     YHFPETGALKSPEILHLLME"
     gene            complement(2765..5083)
                     /locus_tag="PKY63_02140"
     CDS             complement(2765..5083)
                     /locus_tag="PKY63_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPE99443.1"
                     /translation="MRKLLSLLCFQILTTGLISAQGVAVGEWRDHLPYSTVIDLAITG
                     DLIYAATPYSLFYYDVPKWEMVRLSTVQGLSDIGISAIEYSDTYKTLVVAYSNTNIDL
                     ILPDSSVFNLSDIKRKSIPGNKTINNIVINGRYAYMCCGFGIVVVDIVKKEIKDTYYI
                     GNNGSHVNITDIEFFNDSIHASSDEGIFRAHSASPNLAYFGNWNKMHVPVTNANYSDL
                     EVFAGNLFTVQSNPSFNTDTVYYYDGNTWTKFAPFEGVENRGMNVSENKLLICGYNFV
                     NVYDNNLNSLGLVFQYGSLIPNPNQAAYTNDGFLYIGDQNYGLVKTWAYWNYVILRPE
                     GPFSSNAFSLSSNGNIISTVSGGYNESWGSLYRAAEISVFNNEKWNFYYNYITDGLDT
                     ISDFITVQTMPSDAAHIFAGSYGYGLVELKDGHVISVYDETNSTLSPVGTTPDRVNVA
                     GLALDSDNNLWIATTGNNKFLSVRKANGQFKTFTFASAYSFSSASNPVIDQNGYKWIA
                     LPRNEGVFVFNDNNTIDISSDDEYKKLTTAEGFGALPSMSVYSMAVDRDNEIWIGTEA
                     GIAVIYNPENVFDGGSYDAQQILVEVGGYVQNLLESEKVKCIAVDGANRKWIGTEKAG
                     AFLLSEDGTKEIYHFTTTNSPLLSDDINGISIIPESGEVFFATTNGIISFRGTATDPN
                     TSLDSVLVFPNPVMSDFNGYVSVSGLIENGWVSITDMFGTLIFRTRAQGGQAIWNCLD
                     MNGDRPATGVYLIFVTNDDGSVTKATKLMFYH"
     gene            complement(5112..6044)
                     /locus_tag="PKY63_02145"
     CDS             complement(5112..6044)
                     /locus_tag="PKY63_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE99444.1"
                     /translation="MNSSRLAHIALIISGILFGANYWIAKSLMPDYMNPVQIIIFRTA
                     GATLLFALILPFTKKSRFQLKEWVLIFLCGILGVTINQFMFFTGLNLTTPVEAALLHT
                     ISPVIVLILAFFIIRERSTLIQVFGIIVGLGGAVWLTLHGKAISWSSDHLRGDIIIMV
                     NITAYSAYLVLAKPLMTRHDPLRVVFWIFFAGMIAFIPFGFNEAMQMPQLQMPAEMWG
                     ALAYVVIGTTFLTYLLTVFGIKYLSSGTVGAYIYMQPVISGAIGIITGREILDWPKVL
                     AAFLIFTGVFLVSFKKMKSISGNTARAIKKRIRS"
     gene            complement(6503..6790)
                     /locus_tag="PKY63_02150"
     CDS             complement(6503..6790)
                     /locus_tag="PKY63_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99445.1"
                     /translation="MLATVLRSEIAGQISILIISAFVEMRKFVISNKTILSRVNILEQ
                     KQSDTERKFEQVFAAMESGNVNPKQGIFFEGQIFDAWMFVSDLIQSAEKSL"
     gene            complement(6800..7075)
                     /locus_tag="PKY63_02155"
     CDS             complement(6800..7075)
                     /locus_tag="PKY63_02155"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPE99446.1"
                     /translation="MNNKAENIEIVEIENRIFSIRCEQVMIDRDLAELYQIGTKVLNQ
                     SVKRNLNRFPPQWRFQLSDYEVNELVTKCDRLSMLKHSATNPYAFTE"
     gene            complement(7373..7960)
                     /locus_tag="PKY63_02160"
     CDS             complement(7373..7960)
                     /locus_tag="PKY63_02160"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPE99447.1"
                     /translation="MMQTGLELVNPEIENRIFTIRGIPVMLDRDLAELYQVPTKRVNE
                     QVKRNLSRFPENFCFRLSREELNQLVAICDRFQMLKHSSALPHAFLEQGVAMLSAVIN
                     SKVAIQTSICIMNAFVHMRKMRNQNNDLNLRINTLEYRQNETDLKFEKVFNALEARAD
                     APKQGIFFEGQIFDAWMFVTDLIHSTEKSIELWNH"
     gene            complement(7971..8345)
                     /locus_tag="PKY63_02165"
     CDS             complement(7971..8345)
                     /locus_tag="PKY63_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE99448.1"
                     /translation="MKAENSSKKQKQNKEWPSNQVELSGYAGKDPKFQRYGENRLKAS
                     FTLGTHQLFRDSNGQWIRTTVWHNIVAWDHAARKIVDMVRCGSHVSLKGRLRSQETEI
                     GGGKKENRVYILATELTVNLAA"
     gene            complement(8696..8986)
                     /locus_tag="PKY63_02170"
     CDS             complement(8696..8986)
                     /locus_tag="PKY63_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99449.1"
                     /translation="MKKKIWSIIKNKYFIATTVFVVWVGFFDQNNFIHQQSLRSELHK
                     IRKERQFYLDEIHRDTKTYLEVVSNDERMEKFAREKYLMKRDNEDVFLIVYE"
     gene            complement(9065..10084)
                     /locus_tag="PKY63_02175"
     CDS             complement(9065..10084)
                     /locus_tag="PKY63_02175"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPE99450.1"
                     /translation="MENIEWSKLPFGYQKTAKNVRCYYKNGAWSKPEITDSEYMNIHI
                     AATALHYGQEAFEGMKAYRGKNGDVRLFRWDENAKRLARSAQGILMAEVPEDLFFECI
                     KAVVLENLDYLPPYGTGASLYIRPLLFGSGAEVGVKPASEYMFIIFVTPVGPYFKEGF
                     NPVKIAIIKDSDRAAPLGTGTIKVGGNYAASLTGVMKAHKAGYGSPMYLDAKEKRFID
                     EIGAANFFGIKDNTYITPSSSSILPSITNKSLQVLAEDLGLKVERRPIPLDELGSFEE
                     VGACGTAAVISPIGLIEDLETGTKYEFCKDGKAGKWSTALYKKLSGIQYGDEEDKHNW
                     VTILK"
     gene            10276..10992
                     /locus_tag="PKY63_02180"
     CDS             10276..10992
                     /locus_tag="PKY63_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99451.1"
                     /translation="MIRKIMYKSMTLSISLLLLVLLPAIKMLGQSIINLYTPVTVNSP
                     LFSRANEIQFGANLNNFGLNYKVNGQWANTIMIASIQHNPGSIEFDPLNFNDYSDQTD
                     QTHLIQSRPDRMMYCELGLGYNLHHKTQKICFLAGAGRQIIHPNTRWFFQFDWGNESS
                     LINAGITVRANYTKVNGVDFFTMEPMVQGKLKIWNFRIVNQFGYSVAMKKNEDYMKPV
                     LTVGLEYVIGKAARNKGYKE"
     gene            11191..11769
                     /locus_tag="PKY63_02185"
     CDS             11191..11769
                     /locus_tag="PKY63_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HPE99452.1"
                     /translation="MSDQKKNTQGFIPTHGGYRNLFSYQKAEIIYDGTVYFTNRFFSK
                     YDRTVGQMIQAARSGKQNIAEGSMASATSKETEIKLTNVARASLEELRIDYEDFLRTN
                     KLPVWDKNHRLVARIRELNKSTPTPTYETFRKAIEHENPEICANAMITLIQITTFLLN
                     QQIKQLEAAFLNDGGLRERMTHARLQRRNNKE"
     gene            complement(11851..13290)
                     /gene="dacB"
                     /locus_tag="PKY63_02190"
     CDS             complement(11851..13290)
                     /gene="dacB"
                     /locus_tag="PKY63_02190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPE99453.1"
                     /translation="MKKILLILLLHTCLLPMGAQNTLLHQSIENLKNDPYLKQSNWGV
                     IVFNCDQNKVIEEYNADSLLIPASIIKLTSTSTALDVLGDTFRFVTHVYIDGEIGSDS
                     VLHGNLNIVGGADPTIGFPFFTKERFIDSLYAALTRSGIKAIEGNLNGYAGIFDSLII
                     PDTYPDDDRGNYYGAGTAGLIWDGNALTLSFNTPATFGAKTTYVSAFPAFEQFVLENK
                     TTAGRSGTGDNSIVWGDPFDFTRIINGTLPPGKNNFKVYGSTPDPALGFTIAVQSFLG
                     QKGIVVSGKCCGYYLNNGEYGRPLMTYYSQPLSEIIQYTNNKSYNVAAETCLKMSGLK
                     KYGSGSWESGLKAVYDLLGENSVQKNGMELHDGSGLSKANRVSPKAMGTFLMNIHTEP
                     WFEILYASLPIAGESGTLKTMFEGTPVKGKLRAKSGYMKTVRAYAGYVPNQKNEMMAF
                     CIIVNNYSGNAFALKKKLEKLMVNISLVE"
     gene            complement(13287..14750)
                     /locus_tag="PKY63_02195"
     CDS             complement(13287..14750)
                     /locus_tag="PKY63_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPE99454.1"
                     /translation="MNLLKFILIFVFLNQLSSEAQQTELPLLSPAQAKSDISFFENIF
                     FDAHINLALVCDTSMLRYRIDTLQSGITDSISATELYRRLMPVFSSIRDIHCALNLPP
                     ESNDYLQAGKPFLPLDVFISNGSLYVRGDYFDSCITGDKIFSINNIPADSIYPVLMML
                     SSSEGNNIHSREKIAEYYFTSVYPLFFFVDSINTIQTGTDSTTHTCLLPGVDANKEPY
                     HSYFSGAGNESIKPFSFGYSKDRTIAYMRIASFMNGNPGEYHFFLNNAFRNINAKQPK
                     VLILDLRHNGGGFADYGKLLTRFLMQEPYTYVNHLISKSSSIVQHEIIRQTPFQPEIV
                     RLLQCTFGNKAMRALWHKTEGIVDTTWEKQVKPIQSAKNYSGFLVVLFNGMSASTTGL
                     VCNTLRKRPNTLFAGLPAGCAISGTFGQPTPFELPNSGITGTISILRFNQDTAPPVLT
                     PITPDIVLPENSEDLESGNDTQLVEIIHLIRKKISEQ"
     gene            14865..15923
                     /locus_tag="PKY63_02200"
     CDS             14865..15923
                     /locus_tag="PKY63_02200"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HPE99455.1"
                     /translation="MEMLLSLLHTALTLTGIVLVMMLIIEFFNVRSQGKWLTRMQKNV
                     TGQILLGTFMGVLPGCFGTFFIVSLFTHGNVGFGALVATLIATSGDEAFLMFSMFPMQ
                     TVFIHIGLAVVAIIAGFVAHAIFRKKTIMTRKMQFEIHSHEEIPSARNSGIRDNLKNI
                     SFHRAVLIFGLLLVIVNLVLGAAHGHGGGHEMKHFHFEEYIKYIFAGLAFITLLAAFI
                     LPDHFINEHMWGHVIKKHFLKVFLWTFGALIVIEFALPELNAEMWMKENPITLLLIAC
                     LIGIIPESGPHMVFVTMFAGGYIPLGVLMGSSIVQDGHGALPLLAESQKSFLIAKGIN
                     IFVGFVVGYAFILFNIGF"
     gene            15980..18004
                     /gene="ligA"
                     /locus_tag="PKY63_02205"
     CDS             15980..18004
                     /gene="ligA"
                     /locus_tag="PKY63_02205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPE99456.1"
                     /translation="MDRIKFLTTELNRHNYLYYSKDRPEISDYDFDMLLKELEQLEKE
                     TGHVEPDSPTQRVGGQVTKQFDSADHIFPMLSLSNVYSVEELNDFFTRCIKSGGDDVE
                     FTCEPKYDGVAISLVYENGLLVRAVTRGDGSRGDVVTANVKTIRSIPMRLMGSDYPQL
                     FEMRGEIILPFSSFERMNQERFENGDEPFANPRNAASGTLKLQDSAEVAARGLDCMLY
                     SFISDEVVADTHFDVLQKAKSWGFKIHDFIRRCRGVDAVKSYLDEWSAKRNELPFAID
                     GAVIKANDIALQEQMGYTAKSPRWAVAYKYKAEAACTKLLSVEFSVGRTGVVTPVANL
                     DPVQLAGTTVKRASLHNADIISQLDVRIGDSVYVEKGGEIIPKITGVELSLRPDDSVP
                     FEYAKQCPECGTPLQRNEGEAAWFCPNDMECPPQIKGKIEHFISRKAMNIDSLGEGKV
                     ELLYDKGLIKNVADLYSLRAEQLLGLEKSMMDETTGKTRVVRFQEKSVENILNGIENS
                     KSVPFEKVLFALGIRHVGETLAAKIARHVESMDALMKMKAEELVLIEDVGVVIAESIV
                     QWSQSITHMQLVERLKLSGLQFEVKKQALQSEKLKQLTFVISGVFSSPERRNELKKIV
                     EVNGGKVASSVSAKTNYLLAGDNMGPAKRQAAEKLGVKIINEGEFLMMIG"
     gene            complement(18018..20111)
                     /locus_tag="PKY63_02210"
     CDS             complement(18018..20111)
                     /locus_tag="PKY63_02210"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPE99457.1"
                     /translation="MRILIFMAIVGLTTMSCRAQSDIDDIFRPRMPEQIDTIYYPFYN
                     GVQMIEVDHLAYWDRKIFFLEQSQPRAMSYSMLEPPPPPNEYFFRDSTGAIIKAYNTF
                     WSLESLNALFKDIPLDKKSNNLIHQFIPTHAGKVAFSKGVWPYSPQPMNFFNGFYKVY
                     NLINIDTTAKVHSYFQGNDNPYGTSKSGLIDSLGNLVIPMKYDALYPLKNNLLTRLNG
                     KWGVMNKEQKEIIPMEYDSYVWESQELISFSKNDKVKKHYRIDKSTVQDIGDYDEIIN
                     PEYIHQYPVTMVKKDGRIGFIDSSYTEVVAPVYEICESFYASDLKLVRVFRDGKWGYI
                     NKQGKEVIPCQFEDSENFGRDDYALVQFKDEKFCIDTLGNRIQSCDHVVQWQKTNGNI
                     VSRGYVYGLVNNEGIVVLPVIYDQLYKMPNEDFYRISRNKLIGLADKSGKIILPCKYE
                     EIGHFWKDKNIALIRRDTMYGVIDSNFQIIIPCKYELIQFDLSGNLIFKKNGLFGLMD
                     LNTNILIPARYQQFNGFRQIMYTRDPNPYAMVMRDSLYGYIDRHGKEVIPCKYKHLGY
                     EIHNNRVFYQENNKYGFIDTTGKIVVAPVYDRVFDFIIDVTAVQKEEKWAIINDKGKL
                     VTEFIYDMIVGHTWYDQQFVVVYQNKKCGVIDKKGKLVLPIEYEAIHEFSQATGLKPE
                     FRVTKNGSTYWESTK"
     gene            20221..21612
                     /gene="lpdA"
                     /locus_tag="PKY63_02215"
     CDS             20221..21612
                     /gene="lpdA"
                     /locus_tag="PKY63_02215"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPE99458.1"
                     /translation="MNFDVIVIGSGPGGYVAAIRASQLGMKTAVVEKAEVGGICLNWG
                     CIPTKALMKSAQAYKYMKHAADYGIKLEGEVKPDFPAMVSRSRGVADTMSKGIQFLFK
                     KNKIEVLPGFGKLMPGKKVEVTSADGSKTVYQANHIILATGARSRELPALKQDGEKII
                     GYREAMTLPQQPESMVVVGSGAIGSEFAYFYNTIGTKVTLIEFLPNIVPVEDEEVSKQ
                     LERSFKKAGMNILTSAEVTRVDKDGDKCKVYVNTRKGEEVFEADVVLSAVGVATNLEG
                     LGLEETGVASERGKVLVDDYYRTNVEGVYAIGDIVKGPALAHVASHEGIICVEKIAGM
                     DVHPLDYGNIPGCTYTFPEVASVGMTEKAALEAGFEIKTGKFPFTASGKAKAAGNTDG
                     FVKVIFDAKTNQWLGAHMIGDNVTEMIAEAVVARNMKVTGHDIIKSVHPHPTMSEAVM
                     EAVAAAYGEVIHL"
     gene            21641..23503
                     /gene="asnB"
                     /locus_tag="PKY63_02220"
     CDS             21641..23503
                     /gene="asnB"
                     /locus_tag="PKY63_02220"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPE99459.1"
                     /translation="MCGITGVFSKNDNGRKYFESVPVALETLRQRGPDYSATKGGDHF
                     LFGHARLSIIDTSEASHQPFVSEDDRYTMVFNGEIFNFREIRTELEKQGHTFRTSGDV
                     EVVLKSFIQYDVKCFETFNGFFAIGIFDSWEQRLVLARDRFGVKPLLYYSEASVFVFA
                     SEMKAILKYPIRRTLDEQSVYEYFRLTYIPEPDSVFQEVKKLQPAHYAVITRDKTEFN
                     AYYSIADEGFSGSYEDACDRLIELLSDSVRMRLISDVSIGAFLSGGIDSSIISVLAAQ
                     HNPNIHAYSIGFPDAPYYDESVYASSVAKKHGLRHTIIPVTESDFLNALPSVLDYIDE
                     PFADSSAIAVNVLSSFVRKHVTVALSGDGADEVFGGYRKHRALAMIARANRAKMMAYT
                     AAAAIPGVFGRGSRFSDKMRQARRMGRVASMRPEERYLFLAAFHSGREVSHLLHLSRH
                     YSMDRVRNQLDISHPSDMNSVLRNDVKLVLAGDMLRKADLMSMATSLEIRSPFLDHRI
                     IDFAFSLPSKWKFDSLSTKKILTDSYRDILPENVINRPKHGFEVPVGKWMNGALLNEI
                     QTNWFEDKFIRYQGIFQSEKIQRLKHQVERGRGLRDQSLLWSLIVFQSWWKKFM"
     gene            23503..24708
                     /locus_tag="PKY63_02225"
     CDS             23503..24708
                     /locus_tag="PKY63_02225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99460.1"
                     /translation="MAEKIRVLRILNRFNLGGPTYNASQLTKHLEPDFETLLIGGANE
                     KDEHDSLHIPNSLGIEPVIIDSMRRSMNLVLDRAAYRDIKKIIKEFNPHIVHTHASKA
                     GAVGRSAAFNMKVPVIVHTYHGHVFEAYFNGLISSVYKSIERNLARKTDAIIALSERQ
                     KADLTSRFRVCEKDQVHVIANGFDLAKFQEDKKIKRAQFREKYKLRNDEFAIGIIGRM
                     VPVKNHAMFVDVVERVNKLYRGKVRYFIVGDGETCEDTRRRFIQKRIAINDSTEPDTM
                     VHFTSWQKNVDDIMAGLDLLVLTSLNEGTPVCLIEAQASNCPVVSTDVGGIRDIVIDG
                     KTALLSPSQDVETMSRNIVKLITDKKMYESMTLHGWEFVRDRFQYQRMVEDMKALYMK
                     LLAQQKVRL"
     gene            complement(24739..26538)
                     /locus_tag="PKY63_02230"
     CDS             complement(24739..26538)
                     /locus_tag="PKY63_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99461.1"
                     /translation="MKRIISTILLTILCIVSFSQTFEWKRLSDDVVDSVYNQAVFTTN
                     NITLKNQNYYFAYTIFPYEDNWVWPNIFKSGYTYPTILSQDNQIVMADTLYLDSIFLN
                     QNIATTFIQNDTFRVIINYPVLDSATYRDNIILRKGIPVPKTNGFLVINYSLSGNFLG
                     YDSLALPPAIKDLANLSEAASNYALFIKNLLVNSHQEVFVHLCYCLRSGSAGAFPLDT
                     AGFIVKYDSNLSVSNKVIPVLLKNRNYDFPIDSKLHSDKLYVLGSISDSVIVDNMLFT
                     PDTTAGLHTFALEFDRNLFVLQNHKLPGEYNDFAIDESGNFFTLGRFYYPVFMNPDSI
                     IPKKVFVASIDSFVYWNYGLINKLDNNFNSMYYSYFSIPVINNSKFIENENRIFITEF
                     YNGYDTLVDNYPGLGGKENLSFEYDTLAGMFYYKNGDSLESNLIHHFSENLVLLNPEN
                     LVPIWNYDFPESTNVHNLYFNSDKMLMSFDGSYIYLSGFGDTDTVKVYNYYPVALSQI
                     NITGLGFEEMSGSEAAPLVAYPNPASDQLFLKLPENDDFVVSVFDLNGKRQLEQFLSN
                     GENTLNVSALNTGLYIIRAQNKEQILTGKFLKE"
     gene            complement(26828..27415)
                     /locus_tag="PKY63_02235"
     CDS             complement(26828..27415)
                     /locus_tag="PKY63_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99462.1"
                     /translation="MNIVVSCVFSNPAFSQNNNYELNNAKISAPDFYDTIAGKPVQKS
                     VLLLWIDTGYVNPATINDTSNIVRPFGDFVTPAFIDSIPTIILKDFVHAEAKKIFLHR
                     TEYDTVSISRTGKRVPIPPFYTALWVRNSSSNLWGQPHQSGSNWLFTPEDSVMLTYFD
                     GLTAKTTGYVYNLGYYAPLDIGETMHIGLSVYGHY"
     gene            27860..28537
                     /locus_tag="PKY63_02240"
     CDS             27860..28537
                     /locus_tag="PKY63_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPE99463.1"
                     /translation="MFKVKDRASFAPASELLVPVKPLLQTGDNLSINVYSNKGEKLLE
                     PLSTASQQGAGESSPFVFTVDSAGNIELPVVGQMHVAGLTIDQTEKEIAALFANSVVE
                     PYVQISVLNKRIFFMTGGASQAATVIPYVNENTTLIDVITAAGGIREGKAYSIRLIRN
                     TTAGLKIYDIDLSDFAQAKFSFVQVLPNDIVYVEPQHRPFRKFVESLAPYLTLVSTGL
                     LIYNITR"
     gene            28545..30950
                     /locus_tag="PKY63_02245"
     CDS             28545..30950
                     /locus_tag="PKY63_02245"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPE99464.1"
                     /translation="MTDSRKVKFNDEFDFKLFLNITKKSLKYIIVFTILLFAAVFVYL
                     RYTQPVYEAACIVQINTDDKAEKLFDNKTYYEDDIYRKIEIMRSPVFMQRVVSQLPLG
                     VSVFAKGNILNFELYDDIPFDIKYEIIDSTFYGVPLLVEVHDSDFVIAEINGVVTDTR
                     FHFHEPVSLPQVNFVLTPLQNNSRAFFNTNKNKQFIIRFNNLNDIAAQYSQKVNIAVI
                     NDVAKTIRISLKDFNPRKAADIVNSVAKEFAAFEVEYQKNSSSSILEFIDSQLGQVEE
                     NLDETKDSLQQTSSPAIDSTLDRTALQSRLFELDNLILENSAEVEALEVFYKVFSSGN
                     ADIITLLPLISGIESQVYLSPYLQSLKDLEMRKQELLLRVTENSTAIESINAQIDFQN
                     KIIRESTSGLLSKYKRQQQRYTSEKNNILRLLGKPIVGTMDDQSRIERYYSVNEKFYN
                     QLIEKKIEYSIAQAGYVSQLIVLQPAQSSFTPVSPNNKLIWIGAFVFALVMGFAFLVL
                     RYILYNEVVSLRDVKKLTTVPVLGVIPKYKSYIPVSQLIVDTRPRSLLAEALRAIRTN
                     LQFIKNITQSKVIAISSTVSGEGKTFVAINFAGVLAFNNKNVIVLDFDLRKPKIHLGF
                     NTDNEKGISSILLKINTPDECIRHSSIPNLHFITAGPTPINPSELILTNETDDLIAYL
                     KTKYDYIIMDTPPIGLVSDAMKPLQYADYPIYVIRANYSKRSYIYNIEKLYHENNLRF
                     MSIILNNVDPEITGAGSSQGFAYGYGYGYGYNNSRHGYYDSDDSVAKTWREKILDKFR
                     RKR"
     gene            30950..31492
                     /gene="rfbC"
                     /locus_tag="PKY63_02250"
     CDS             30950..31492
                     /gene="rfbC"
                     /locus_tag="PKY63_02250"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPE99465.1"
                     /translation="MEVLRFECDGLILIKPKVYADDRGHFLETYHWQKYRELGINSDF
                     VQDNESLSHRLVLRGLHFQSPPFAQGKLIRVLRGSIYDVAVDIRKDSLTFGKWQSVVL
                     SAENKHQFWIPEGFAHGFLALEDNTVINYKCTANYNASAERTLLWNDTDLNIGWPETP
                     LIVSSKDKAGEIFRDFVSPF"
     gene            31504..32565
                     /locus_tag="PKY63_02255"
     CDS             31504..32565
                     /locus_tag="PKY63_02255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPE99466.1"
                     /translation="MVWNDIVLVVVLFFGFSLIINGLFLKFSSTLGLRTAKEAGVRWS
                     ASQKPSFGGITFYILFLISVISYSMLFPNAAMVRNVEFLGLMLAVSIGFIAGLFDDAF
                     NTKPLIKFGLQILTAVVIIFSGSAICITGNMYGDMILTAVWVIGIMNAINLLDNMDAI
                     ASVVVIFILACFLVIMQLNGLNDGPYLFISIGLIAAVGGFLFFNWNPSKLFMGDTGSM
                     FLGTAVAFLGIKYGWNIQLENAVVADWQKVLTVVVVFIVPLTDTTTVFIKRIFSGHSP
                     FIGGKDHTTHHLSYLGLSERAVAMAYCLIGLLSLLAIVKLLSLFSHWNGFLTLAYVSY
                     IVIVFGGLFYIAHLNRDRE"
     gene            32685..33680
                     /locus_tag="PKY63_02260"
     CDS             32685..33680
                     /locus_tag="PKY63_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPE99467.1"
                     /translation="MKKWIIGLIVMLILAIGGMQLFMFSAVNSNPRNPDEQYFSMSRQ
                     TVKVFQPYIPDSLHFCGEKVPTDHLIVRERLEREIMANMYWHSHTILLLKRSGRYFPL
                     IEPILKENGVHDDIKFLAMCESELTNAVSPAGAKGFWQFLHKTGLEYGLEINSSIDER
                     NNLEKSTVAACKYIKSSLSRFGSYSLAAASYNMGSGGLQQKMNEQKCNDYYSLFLNEE
                     TSRYVYRILALKLIYNNPLTYGFFIRNKDIYRPIKVKHIEVSASIPDLAVWSKDKGVS
                     YLELRYLNPWIISNSLNVSAGKTYRIALPVSMSYSESIEEIGSPFTIFQDSVSVE"
     gene            33684..36551
                     /gene="uvrA"
                     /locus_tag="PKY63_02265"
     CDS             33684..36551
                     /gene="uvrA"
                     /locus_tag="PKY63_02265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPE99468.1"
                     /translation="MSKDDFLKDSGQEWLEVIGAREHNLKDVSVRLPRYSLVVITGLS
                     GSGKSSLAFDTIFAEGQRRYMDTFSAYARQFIGDMSRPDVDRITGLSPVISIDQKTSG
                     RNPRSTVGTITEIYDYLRLLYARASTAYSMHTGKPMVQYSDARIAEMIGQRFEGRQIA
                     VLAPLVKARKGHYRELFEQYRKLGFVFFRIDGEITEVAQGLQVDRYKIHDIELVVDRI
                     EMSGTSNKRLIKALDLALRYGKGVVAIHEEQSVNTAFFSRKLMCEDTGESYELPEPNS
                     FSFNSPYGACPHCKGLGTVSEVDMKKIIPDKRVVIRKGGIAPLGEYKNNWVFKQVELI
                     CKKYGHTLDDEVGDLSDDALNAILYGSNEMLHLTNDYLGVTSSYNLSFDGIINMLVNR
                     QDDDMNPALRRWAESFMNMIPCPECGGTRLKKESLHFRIDGKNIAEVSDFEMDHLLEW
                     VGHLPQKMEKRHAAIAVDIIRELTSRIGFLSKVGLNYLSLNRPASSLSGGESQRIRLA
                     SQIGSKLTGVLYILDEPSIGLHQSDNRKLIESIRRLRDAGNSVIVVEHDEEMIRSADY
                     VIDMGPGAGRKGGFITFAGVPADMDNHTTITCDYINGVKSIPVPEIRRPIDSRRISVK
                     GAEGHNLKNLQVDFPLGLFVCITGVSGSGKSSLVNETLYPVLSNKLYRSNARPLKYKS
                     IEGLDHIDKVIEIDQAPIGKTPRSNPATYTGVFDEIRKLFSVLPESKARGYKPGRFSF
                     NVAGGRCETCKGAGLKTIEMNFLPNVYVNCDDCGGKRYNRETLEVRFKGKSISDVLDM
                     TINQASEFFENQSSILTYVSTLQKVGLGYIKLGQSSTTLSGGEAQRIKLATELTRRDT
                     GRTLYILDEPTTGLHFEDVRVLLEVLNTLVKRGNTVLVIEHHLDVIAYADYIIDLGPG
                     GGKHGGRIVAKGTPEEVSNVNDSLTGQYLKEHFEQRKKIKK"
     gene            36564..37298
                     /locus_tag="PKY63_02270"
     CDS             36564..37298
                     /locus_tag="PKY63_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPE99469.1"
                     /translation="MMNDNNGFEHLNDDEKIRRALEPKNWHEVKTHDSWQIFKIMSEF
                     VEGFETLATIGPCVTIFGSARTLTGDPYYKLAVDIAYKLCKAGYGVITGGGPGIMEAG
                     NLGAKKAGGKSVGLNIQLPFEQEANEYIDKDKILNFKYFFVRKVMFMKYSQGFIVLPG
                     GFGTCDEMFEALTLIQTKKIAHFPIVLVGKEYWTDLDKWFKDTMLKFGNIAEKDLDLY
                     SFAETPEEAVAVIENYYRKYNLSPNF"
     gene            37301..37774
                     /locus_tag="PKY63_02275"
     CDS             37301..37774
                     /locus_tag="PKY63_02275"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HPE99470.1"
                     /translation="MKSILSLRFLTAMEGQQSHIAVDAVINGKPALMIIDTGASNCVL
                     DSARAVKFELLHDPFFISDSAIGIGSGQLSVTLSRSEYFELGTFRIELFPFVLLDLAT
                     INATFAKAGCEPVDGIIGTDLLLAANAVINYKSATITFRGNKKVLKKLFQNISLS"
     gene            37906..40995
                     /gene="secDF"
                     /locus_tag="PKY63_02280"
     CDS             37906..40995
                     /gene="secDF"
                     /locus_tag="PKY63_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPE99471.1"
                     /translation="MQIKGTIRFFAVIFILVSVYSLSFTYCTRKVEKEAKEYATSEAV
                     LQLSKEIAEGDSLRERVVFDSISAAWESYYLDSMSNEVIYNLLVKKYTYKDCKEKELN
                     LGLDLKGGMNVTLETSEADIITALSGNNQDPNFKKAIKNASEKQKNSQSAYVDLFVES
                     IRELAPGKPLASFFLTRELSERIKTNSSDDEVITVLNEEISSAIDRTFMVLRKRIDKF
                     GVTQPNIQKLSTSGRILVELPGVKDPTRVRKILQGTAKLEFWETYDFVEIWPALEKAD
                     QTIKNMISKQLVSVDSSVTDTIVAADTTVATAQTQSNTLEEKLDQIPGQTSTTQDSSD
                     VKSQSPLISLFASFNIVESEGKRFAGEGPLIGYVHSKDTAQVNAYLRMVQSSFTNTTF
                     AWGAKPVPMDIPGTFYELYALKISTTDGRPKLEGDKVADAWQDYDQNGRVEVSMMMTS
                     EGAKVWKKLTADNVGKSIAIVLDNYVYSAPNVMGEIPNGRSSITGQFDVAEAQDLATV
                     LKSGKLPAPARIVEEAVVGPSLGIEAINAGMLSFVISFVLVLIYMIFFYSKAGIAANI
                     ALLTNIVLIFGILASMGAVLTLPGIAGIVLTIGMAVDANVIIYERVKEELRAGKGTRL
                     AIEDGFKNALSAIIDGNVTTLLSGFILLWFGSGPVQGFATTLIIGIFTSLFTALFITR
                     LVFIWWLDRNKPVAFFTKITEKLFTATKFDFIKMRKWAYVFSGLIFVAGIASIATRGM
                     SWGVDFSGGRTYVVRFDNDVQVDDIRGELFKNMGETPDVKTFGSNNQVKITTKYMVDD
                     NSENADSLVEAKIFESLKGFFNTSMTFKDFTSDDPDKVLGRLSSQKVGPTIADDIKQS
                     SVYALILALIVIFAYIAIRFKNWQFGMGGVVALFHDAMLAIFMFSWFHGILPFTLDVD
                     QTFIAALLTIIGYSINDTVVIFDRIREYGVLYPTRDNRTNINDAVNSTIGRTINTAGS
                     VIIVLLAIFILGGEIIRGFSFAMLVGTISGTYSTLFIATPLAYDMLKRKEAKKKLKA"
     gene            41194..43380
                     /locus_tag="PKY63_02285"
     CDS             41194..43380
                     /locus_tag="PKY63_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99472.1"
                     /translation="MKNRFIFYLLVLAVVAVAPVKQSMAQKNFTAEADDKFALNKYYD
                     AIELYKKAYTKVKNKVEKNRILFQIGTCYYLINDMKNAGLNMKRVIKANYPEVEAYNI
                     YAQSLKIQGNYEQALIAFNDFKAKYPDDKRADAGIESCKLAISWLDAPTRYTVEPDKK
                     LNSRNADFSPAYFDKKYKAIVFTTSREEVNGRGEDNWTGLPFTDLFVTSQDKKGNYST
                     PVPLDEEGIVNSEANEGSCTFNSKFNTIYFTRCRKEKKKILGCEIYSSVKKGRSWAEP
                     ELIPIAPADSFTVGHPSLTDDELTMYFASNMPGGQGGRDIWMITRPKKNKPFEKAVNL
                     GEIVNTPGDEMYPTIREIGGKKYLYFASNGHIGMGGLDIFRSEFVDGKWTAPVNMKAP
                     LNSSSDDFAIIFNDDPKMLKEADAKEMGWFTTNRKGGRGNDDLWRFKLPPILFTLAGV
                     VRDDSTKQILKGAEVRIEGSNGTIYTDSTDQTGSYYFDNIQIVENTTYNMMVALPDYY
                     SEKGKETTVGLEVSKDLVHDYYLVPIPKKPIVLPDILYDLARWELKPQYEDSLDGLVT
                     IMKENPTLVIELGSHTDIRSSDDYNDTLSYKRAKSCVDYIISQGVEPDRLVPKGYGER
                     VPRVLESDKLVVYKGKEYRFSKGQVMTEDFINGLATDGEKEAAHQLNRRTTFQILRSD
                     YVPKANTNTPVNPEIQIINEGGEDEGGEDEGEDTNTENPGGGEGGN"
     gene            43501..44661
                     /locus_tag="PKY63_02290"
     CDS             43501..44661
                     /locus_tag="PKY63_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPE99473.1"
                     /translation="MNNDKYLHRGVSSGKEEVHAAAGKIGHGSYERAFCRADADPSDP
                     EKLIVLHADGAGTKSALAYAYWKQTGDLSVWQDIATDAMVMNTDDMLCVGIDNHFFVS
                     STIGRNKQLVSGDVIAALIEGNIRFRDKMKRYGIDVVLSGGETADVGDLVRTIIVDAT
                     ALGFVNRKDFISNEKISAGDVIVGLSSYGQCEWEDRYNSGIGSNGLTSARHDIFDFSV
                     GQTFPETYDPIIEAFAYLGRYKLTQASPVEGLDMGKFVLSPTRTYLPLMKKVFEKMRS
                     SVHGLVHCTGGGQFKCIKFIDQLKVIKDNFFEVPPVFDLIMKSGTEAEEMYRTFNMGH
                     RLEIYTDEKSAKEMMKMAGDCGIDARVVGYCETSDKAEVELKTPFGTFKKEV"
     gene            44665..45744
                     /gene="prfA"
                     /locus_tag="PKY63_02295"
     CDS             44665..45744
                     /gene="prfA"
                     /locus_tag="PKY63_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPE99474.1"
                     /translation="MDILGKLKEVHEKWEEIGRMLIEPDVIADMKRYVALNKEYKELQ
                     SVSEAYLDYSNVLSNIDNAKNILQTEKDDEFREMAKAELDELLMKKEQMEEEIKILLL
                     PSDPQDRKNCIIEIRGGTGGDEASLFAGDLFRMYTRYLENRKWKYEIVDFTEGTMGGY
                     KEIIINVNADSAYGTLKFESGVHRVQRVPETEAQGRIHTSAATVAVLPEADEVDVDIK
                     ESDIRKDTFCSSGAGGQSVNTTYSAIRLTHIPTGIVVQCQDERSQIKNYDKALKVLRS
                     RIFEIEYQKYLDKIASQRKTLVSSGDRSAKIRTYNYPQSRVTDHRIGMTVYNLPSVMN
                     GDIQQFIDALQIAENTERLKEGVNI"
     gene            45757..46599
                     /gene="pyrF"
                     /locus_tag="PKY63_02300"
     CDS             45757..46599
                     /gene="pyrF"
                     /locus_tag="PKY63_02300"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPE99475.1"
                     /translation="MNRQELTNLVFKKKSVLCVGLDSELSKIPAHIKEGRSIEESVFE
                     FNKAIVDATIQYAVAYKPNLAFYEALGLSGMTALHKTVAYIRSLSEPVFIIADAKRAD
                     IGNTSRLYASAFFGDGKTAPDFDAVTVAPYMGKDSVSPFLSFDGKWVILLALTSNPGA
                     DDFQLTATPDGMQLFERVLQQSKQWGNAENMMYVVGATRADMLKKVRNIVPENFLLIP
                     GVGAQGGDLNEVLKNGWNNSGGLLINASRSIIFASSGGDFAEAAAKEACRMQNEMAQF
                     INQL"
     gene            46602..47324
                     /locus_tag="PKY63_02305"
     CDS             46602..47324
                     /locus_tag="PKY63_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99476.1"
                     /translation="MRFYALFLFLIPLSLFSQGDDWFDNIENITDNVEEPMLYYERFN
                     AFTMGDSVRLCNKRPCNGIQKDFWENGIIKHKAYYQNGRITNGYENYFDNEQIERKFT
                     IINASSAELVVYYKDGTLRSKVEYRRKNAVKWADYYPNGNLEFIEEYDNSMEYYIKYN
                     FYYMSGSPQSTLELVDKNERIYTSKTYYKNGQLKEEGFRQLNMAAGDYVRIGEWKYYD
                     ESGKLIETQNFFKGEEIDEEDE"
     gene            complement(47406..49394)
                     /gene="gyrB"
                     /locus_tag="PKY63_02310"
     CDS             complement(47406..49394)
                     /gene="gyrB"
                     /locus_tag="PKY63_02310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPE99477.1"
                     /translation="MTPDSENTEVKDKKIKNNGNDYSADSIHVLEGLEAVRKRPAMYI
                     GDIGQRGLHHLVYEVVDNSIDEALAGFCNNIDVIIRKDNSIMVTDNGRGIPVDFHEKE
                     QRSALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSSLIATVHRDGKIYQQN
                     YSCGKPLTGVEEVGTTDKTGTVIEFLPDDSIFTIKEYVYDILASRLKELAFLNKGIRL
                     TLTDEREEEDGKPVFNEYYSANGLRDFINYLDGTREKLIDEPIYFEGERGGVPMEIAM
                     QYNTSFSENVHTFVNNINTIEGGTHLAGFRRGLTRTLKAYAEKSGMLSKLKFDINGDD
                     FREGLTAIISVKVQEPQFEGQTKTKLGNNEVMGVVDTVISEYLNYYLEEHPKEAKAIV
                     NKVILAATARHAARKAREMVQRKNILTGSGLPGKLADCSEKDPAACEIFLVEGDSAGG
                     TAKQGRNRQFQAILPLRGKILNVEKAMAHKIFDSEEIKNIFTAFGVTIGTEEDSKELN
                     LTKLRYHKIIIMTDADVDGSHIATLILTFFFRYMKQMIELGYVYIATPPLYLVKKGNK
                     EQYAWSDAQRDALTEQFKGEGGTDKNIHVQRYKGLGEMNAEQLWHTTMSPETRTLRQV
                     TIENGAEADRIFSMLMGDEVPPRREFIEKNAKYARIDA"
     gene            49751..51379
                     /locus_tag="PKY63_02315"
     CDS             49751..51379
                     /locus_tag="PKY63_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPE99478.1"
                     /translation="MKKLNFFELSGVIRIMLMSILFSWTGSMANNVRITNVTNTNPGA
                     ADPIISFDIAWDNSWRVSSGPSNYDAVWIFVKYQKVPTGSPNCESYQEWHHAKMTNVP
                     GDFSVGAPLDVVFVGDSMGIYVQRSADGIGNVSSTTVTIRLNLDVPVSPFDPEMNFKI
                     FGVEMVYVPQGDFELGDGISTNSFNSISITNTTTTLPAATVGGSVHSTIPSAFPKGYS
                     AFYMMKYEVTQQQYVDFLNTLTFNQQLNRTILTSTQLATDPGLGGVCAMISGCLNRNS
                     IKLIQEGINDSRPGVFACDFQPAPGDPFSSINDGQSIANNYMSYGDLLAYLDWAALRP
                     MTNFEFEKTSRGPLSRVGNEYIWGSTDITQAQSTSLNSAGTASETSTTAGNGLSAYGS
                     ASTSGPLRVGFSATASTDRVTSASSYYGVMNLGGNVWEMVAGGSNSVNTGYTLTYSSL
                     GDGELTAAGLYNTSGWQTYNSISYSSLGCGCCSSYIKWHMELRGGSFMSASTTLRTSD
                     RSKNSSVTCGGGPDFGFGNAGDQRQSDVGGRGVR"
     gene            51404..>53811
                     /locus_tag="PKY63_02320"
     CDS             51404..>53811
                     /locus_tag="PKY63_02320"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPE99479.1"
                     /translation="MKRIRSPIFLLLFSLISSLGFAQYAGGDGYGSISLLLSQTSCST
                     PVYPSIFTGGDETCAAAFQLQQASCAAAALPSVFAGGDENSASSMLFSQVSCAIPVYP
                     SIFNGGIESSESSLNVTQVSCAAPVYPSIFSGGSDDGFSGTPTIQVACITPPDAGIYA
                     GGNSNVTTGVTFLTACAPYAEFEADTQFVCEGDTVFFTDLSLGGPTVWSWSFEGGTPL
                     GSSLQNPFVIYNTAGTYQVQLQISAPTGNSTELKTGYIVVGASPAATIAASGPTSFCG
                     GDSVQLTVNPAHPSYAWNTGQTTQSVYASVSGNYYSVVTAANGCTANTDTISVNVLTN
                     PVPLVTTSGPTTLCNADTLTLTSSPGTSYLWLPGSETTQTLEVTASGTYYVSVSYPNG
                     CSNVSAPVNAVFGTTPATPVITPSGPLTFCSGDSVVLTSSPASSYLWNPGSTTAQTIT
                     VKNSGTYYVETGNGTGCTAMSLPVTVTVLPNTPAPVITASGPLTFCTGDSVVLTSSPA
                     ATYNWSPGAQTTQSITIHTAGTYFVEADNGNNCPSVSTPVTVTINAVPATPIIGASGP
                     LTFCAGGNVVLTASAAGSYHWYPNGETTQSITVTVSGNYYVETGNGTGCTASSTPVSV
                     TVNAVPSTPVVSASGPLTFCNGSSVTLTSTAATSYYWYPNGETTQSITVSASGNYYVE
                     TGNGTGCTASSTPVAVTVNAVPAIPTIGASGPLTFCAGGSVVLTASAAGSYYWYPNGE
                     TTQSITVTASGNYYVETGNGTGCTASSTPVSVTVNAVPSTPVVSASGPLTFCNGSSVT
                     LTSTA"
     assembly_gap    53812..53911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <53912..55342
                     /locus_tag="PKY63_02325"
     CDS             <53912..55342
                     /locus_tag="PKY63_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99480.1"
                     /translation="VTVNAVPSTPVVSASGPLTFCNGDSVVLTASAAASYQWYPGGQT
                     TQSITVFAPGNYYVETGNGSCSAQSATTSVAVLPRPAVPVVSASGPLVFCDGDSVVLT
                     SSLADAYEWNPSGGTSQSLTVTASGTYFVEVTNASGCSNSSVPVTVTVNPNPAVNLTG
                     PSVACLNTTENFTTTNLGGVSYFWTTGNGTIVSGSGTNAVSVLFPDTGQASVEVIVSD
                     LATTCFSQAEIILLVLNAPEAYAGADVSVCQGSSVQLQAFGGTTYSWTPPAGLSDPDI
                     ANPLATPVSTTTYIVEVANGSCTDVDTVLVTVYPIPAVDAGSDVYISEDSCVVLAGSG
                     IGSALWTPAYGLSETNTLTPTACPDTTVTYYLTVTGAGGCFATDSVTVYVSASGDELV
                     FPNTFTPNADGTNDIWYISGLEQYPSHRLTVFNRWGNQVFDAEPYENNWDGTSLGREL
                     PDGTYYYVFDKGNGEDLLKGFLMIIR"
     gene            55353..56261
                     /locus_tag="PKY63_02330"
     CDS             55353..56261
                     /locus_tag="PKY63_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPE99481.1"
                     /translation="MKKIWILIFVISVQTAFAQQLPLLSQYSFNKYVFNPAAAGNEDR
                     FEILTGYRSQWTGFNGAPQTASFTAHGPLGGGSCIGGDAYSDELGATSELGFHLTYAY
                     QVQVSNDVRWAFGLQAGAVQYSVDGTLLTTYEPNDQVVPLNMTSALIADATFGTYLFS
                     EKFFAGASIQHLAGSSILFSNDFPKDQVGLLDRHLVLLGGIKLDAGDKLAVEPSFMLR
                     AVTGAPLQIDFSSRLIYDNKYWFGLTYRTRAAWVAMIGLDISDHFVLGYAFDYATTEL
                     QSFSDGTHEIILGWKFNKKQRSNMFI"
BASE COUNT        15547 a        11332 c        12938 g        16737 t
ORIGIN      
        1 tgccacatcg gacgtatgat tatttataat gttgccgatg tgaatgatta caaaaaatca
       61 ccacagttgc ctttgcataa atcggacaag ccattttatt tttttgaaaa actacttccg
      121 gagctcgata caactgtatt tccgaaacgc ttcattactg aagatgaaaa aattaccaac
      181 tgggaaagtt tgtttttgga aacaaaaacc acgtcgtttc tgatcataaa aaatgacacc
      241 atcaaatatg aaaagtattt tcttggccgc gatgaaaaca gcattatgac ttcgttttcg
      301 gccaacaagt cattcatttc tgctttggtt gggatagctg tagccgaagg gaaaatcaca
      361 tctatcaagg atcccattac aaaatatctc actgatttca aaaacccggg atttgaaaaa
      421 attaccatcg agcacttgct caacatggag tcgggcattc gtttccgtga aaactatttt
      481 aacccttttg cagagattgg caaatattat tatggcaata atctgaagaa atatgttccc
      541 aatcttaagg ttgaaaaaga cccgggaact gaattccaat accgcagtgt gaatacattg
      601 ttgcttggaa tgattgtgga aaaagcaagc ggtgttccga tcggacaata ttatcaggac
      661 aagctctgga caccgctggg gatggaatgc gatgccacac tgaatactga ttcaaaaagc
      721 ggaaatcagg tgaaatgcta caccgggctg aatgcaagat cgattgatta tgcaaaattc
      781 ggacgtcttt atctgaacaa aggccaatgg aacggacagc agatcattcc cgaagaatgg
      841 gtgatggcca ccattacgcc gattgtgaag gagcgctctt ccgaatatta caaatatcag
      901 tggcgtattg ataatgcagg taatttctgg gctgcaggtc tgctcggaca atatattttt
      961 gtgaatccgc gcaccaatgt aatcatagtc agaactggca agaaaaacgg ctctgtgaat
     1021 tggcccacgc tgatggcgca catttcgaaa aaaatctgat atgcctaaaa tcggcgttct
     1081 atcggataca catgggtctc tgcctgattc ggtgggcgaa ttttttgccg gatgtgatga
     1141 aatctggcat gctggcgatg tgggcaatga tgctgtattg aaacagcttg cttctattgc
     1201 atcggtgcgt gttgtttacg gaaatatcga cgggcaggat gtacgaggcg ttgcgcgcga
     1261 ccacacattt tttatcagtg gtggaaaacg tgtattgatg aagcatattg gaggccggcc
     1321 tggagcatat acacccgaaa cacgcgagca gattgcagct ctgaagcccg atatttttgt
     1381 atgcggacac agtcatatcc tgctgattca gttcaatcag aaaaacaatt tgttgcacat
     1441 caatcccgga gcagccggca gcagcggctt tcataaaatg atgactatgc tgcgttttga
     1501 tatcagcgac ggcaacattt taaatatgga agtgtgggaa aaaccgcgca aggcgcttat
     1561 ctgagccggt cagcagcgcg gactttcatt tcatcttttt tgatggcttt gttggcaaac
     1621 accagcagaa ccatgctgat gagcggcata atgattccaa tttctttata gtaagcatgg
     1681 ccgtgaacag catcggccag acgctcggga acaataaaca tggcgcctat gagcagacag
     1741 ttgagaataa aaacaaaggc attgaaccgc atttgtctga tgcggttttt gaacagaaaa
     1801 atagaaatca ggaaactgac ggcggtaata gtgcctgcaa atagcagcag tagtggattg
     1861 taaacacctg ccaccaaaac atctcctttg cattcgtgca gatagagata gccggcagca
     1921 ctgtcggcaa tgactctgga aaacggaata aaaatcatgg ccagaatggc aagtgcgcca
     1981 ccaagcagat agagtgattg aattcgctga atcatggttt caaattttga caaaaatagc
     2041 gatttttcat tccatgagca aatggagaat ttccggcgat ttcaatgctc ctgtttcagg
     2101 aaaatgatag cgaaaatatt gcagcatgac ttgaaaaatt tcttttagag atcgtgatgg
     2161 aatgcgggtt tcaaagggta tattgatttc ggctgatatt atttcactca aaaattccga
     2221 atttgtctga tttaatgcgg cctgtgttgt tccgggaaca aatcctttgt cgagtgtgaa
     2281 aattattttt tctttggcat ttgcagtact gtcaattcca aatccaagca gagaagccag
     2341 tccgaagcca aactgaacag ggtatgcagc cggaagatat tcgctttcat gtacaaaccg
     2401 gatgcattgt tccatataga taaacagttc cggggtcatc gattcctctc gaatgtattt
     2461 tacggcacat tcagccatga atgttgaaat ggcctgacgt ctgaaatcaa aagcaccata
     2521 tcgtggtaca taatcgagcg acaatgattt taccagtgga agttttccgg gtgtgccaga
     2581 gatatattgg cattcggtaa tagcaagagg gtatgaaaga aacggatatt tgtttttatt
     2641 gttttttaag taggacgcga caaaaaatgt acgaattcct tttgcagtca gacttttaat
     2701 gattaatcca ttatcaccgt attttacggt atgcaggata atacaacgga cagcagaaat
     2761 catatcaatg ataaaacata agtttggttg cttttgttac cgagccatcg tcgttggtaa
     2821 caaagattaa atagacacct gttgccggac ggtcgccatt catgtcgagg caattccata
     2881 ttgcctggcc gccctgtgcg cgggttctga agatcagagt gccaaacata tccgtaatgc
     2941 tcacccatcc gttttcgatg agacctgata cagaaacgta tccattgaaa tcgctcataa
     3001 caggattggg aaataccagc acggaatcaa gcgatgtatt gggatctgtt gccgttccgc
     3061 gaaatgaaat aatgccgttg gttgtggcaa aaaacacttc gccgctttca gggatgattg
     3121 aaatgccgtt gatgtcgtcg gaaagcaagg gtgagttggt tgttgtgaaa tgataaattt
     3181 cttttgtgcc atcttcgctc aatagaaaag ccccggcctt ttcagtccca atccatttac
     3241 gattggctcc atcaacagca atgcacttca ctttttcaga ttcaagcaga ttttgcacat
     3301 aaccaccaac ttcaaccagt atttgttggg catcatagct gcctccatca aaaacatttt
     3361 caggattgta aatcacagca atacccgctt ctgttccgat ccatatttcg ttgtcgcggt
     3421 caacggccat actgtaaaca ctcatggatg gaagagcgcc aaatccttcg gccgttgtaa
     3481 gttttttgta ttcgtcatca gaagaaatat caatggtatt attgtcattg aaaacaaaaa
     3541 cgccttcgtt gcggggcaat gcaatccatt tgtagccgtt ttgatcaatg accggattgg
     3601 atgcggagct gaaactatat gctgatgcaa aggtgaaagt tttgaattgc ccattggctt
     3661 ttcttacact taaaaatttg ttattgccgg tggtagcaat ccagaggtta ttgtctgaat
     3721 ccaatgcaag acctgcaaca ttgacccggt cgggagttgt tccaacaggc gacagggtgc
     3781 tgtttgtttc gtcatagacc gaaatcacat gcccgtcttt caattccacc aatccgtatc
     3841 cataggaacc ggcaaatatg tgagcggcat cggatggcat tgtttgaacg gttatgaaat
     3901 ccgaaatagt atcaagtccg tctgtgatgt aattgtagta gaaattccat ttttcattat
     3961 tgaataccga aatttcggca gcacggtaca agcttcccca cgattcgtta taaccgccag
     4021 agacggtgga aataatatta ccattggatg aaagcgaaaa agcattggac gaaaaagggc
     4081 cttcaggtcg aagaataacg tagttccaat atgcccaggt ttttaccaga ccatagtttt
     4141 ggtcaccgat gtacagaaaa ccgtcgttgg tatatgcagc ttggtttgga ttgggaatca
     4201 aactgccata ttgaaaaact aatccaagcg aattcagatt attgtcgtaa acatttacaa
     4261 aattataccc gcatatcagc agtttatttt cagagacatt catgccgcgg ttttcaacgc
     4321 cttcgaacgg ggcgaatttt gtccatgtat ttccatcgta ataataaaca gtgtcggtat
     4381 tgaatgacgg gttggattga acagtgaaaa gatttccggc aaacacttca agatctgagt
     4441 aatttgcatt ggtaaccggc acatgcattt tgttccagtt gccaaaataa gccagattgg
     4501 ggctggcact gtgtgccctg aaaatgcctt catcggatga agcatggata gaatcattaa
     4561 aaaattcaat atctgtaata ttcacgtgac tgccgttgtt tccgatgtag taagtatctt
     4621 ttatttcttt ttttacgata tcaacaacaa caattccgaa gccgcagcac atgtaggcat
     4681 aacgcccgtt gatcacaata ttgttgatgg ttttgtttcc gggtatgctt tttcttttta
     4741 tatcagacag attgaaaaca ctgctgtcag gtagtataag atcaatattg gtgttggagt
     4801 atgcaacaac aagtgttttg taagtgtctg aatattcaat tgcagaaatg ccgatatcac
     4861 ttaatccttg tacagttgaa agccgcacca tttcccattt tggaacgtcg taataaaata
     4921 atgaataagg tgtggcggca tatataaggt cgccggttat ggcaaggtca attacagtgg
     4981 agtatggaag gtgatcgcgc cattcaccaa cggctactcc ctgggctgaa atcagaccag
     5041 tcgtaagtat ctgaaaacaa agcagtgaaa gcagttttct catataaaca aaagtaatgt
     5101 ttttttgttg attaacttcg gatccgcttt tttattgccc tggctgtgtt gcctgaaatc
     5161 gatttcattt ttttgaagct taccagaaaa acaccggtaa aaatgaggaa tgctgccaga
     5221 acctttggcc agtcaagaat ttcccggccg gtgataatgc caatggcgcc gctgattacc
     5281 ggctgcatgt agatatatgc accgactgtt ccgctgctta aatattttat tccgaaaacc
     5341 gtaagcagat aggttagaaa tgttgtaccg attactacat aggcaagtgc gccccacatt
     5401 tcggccggca tctgcaattg cggcatctgc atggcttcgt taaatccaaa agggataaag
     5461 gcaatcatgc ctgcgaaaaa tatccagaat acaactcgta agggatcgtg acgtgtcatg
     5521 agcggcttgg ccagcaccag atatgcactg tatgcagtaa tgttcaccat gatgatgata
     5581 tcgccgcgca gatggtccga actccacgaa atggctttgc cgtgaagagt aagccataca
     5641 gcgcctccaa gtccaacgat gattccaaag acttgaatca atgtggaacg ctcgcggatg
     5701 atgaaaaatg ccagaatcaa aacaatgacc ggactgatag tatgcagcaa tgccgcttct
     5761 accggggtcg tcagattcaa tcccgtaaaa aacataaact gattgatggt gactccaaga
     5821 attccacata gaaaaatgag tacccattct tttaactgga aacggctttt tttggtgaaa
     5881 ggcagaatca gagcgaaaag caaagtggct cctgctgttc tgaaaataat aatctgaacg
     5941 ggattcatat aatcgggcat caacgatttg gcaatccagt agttggcgcc aaaaaggatc
     6001 ccggaaatga tcagtgcaat gtgagccagg cgggaggaat tcatacggca aagataatca
     6061 gtaactcctt gctgaaatac catttttatt tcggaatatt ttttctgaat acgtcgatca
     6121 ttttttcatc caacagtgaa aatgcgaaac agcgcttgcc gagatctttc agcgatgctc
     6181 cgacgtgata cagccgttta ttgtcaataa gaagaaagcg gtcatgagct gacaagattg
     6241 ttaaaattaa caaaagaaat acaactgagt tcccggtctc tgcgccggga tctctttcta
     6301 cacctcagac ttttcttttg ttgattttcg ttaccgacat ttcgatggag tttatcctgc
     6361 tgtcgactct tcgacggagt ttatcctgcg gaacgcaggg ctcagagtga cagtagcagg
     6421 gctccatgtg acagcggcgg gtctcaggat gacaacgagg gagagcgttt tttactatgc
     6481 gcgctttgaa acaaatccaa aactatagcg atttttcagc agattgaatc aaatcagaga
     6541 caaacatcca tgcatcaaaa atctgtcctt cgaagaagat gccttgcttt ggattgacat
     6601 tgccagattc catggcggca aacacttgtt caaatttccg ctctgtgtcc gattgttttt
     6661 gttctaaaat attcacgcgg ctcagtatgg ttttgtttga aataacaaat ttccgcattt
     6721 cgacaaacgc gctgatgata aggatgctta tttgccctgc aatttcactg cgcaacactg
     6781 tcgcaagcat ggcgactccc tattctgtaa atgcatacgg attcgttgcc gaatgtttca
     6841 acatcgacaa ccggtcacat tttgtgacca gttcatttac ttcgtaatca gatagttgaa
     6901 agcgccattg gggcggaaag cgattcaggt tcctttttac cgattgattc aaaacctttg
     6961 taccaatctg atataattca gcgagatcgc ggtcaatcat cacctgctcg cacctaattg
     7021 aaaatatgcg gttttcaatt tcaacaatct ctatattttc agccttgttg ttcatttatt
     7081 ttttaattgc tcagaatgtt ttttatgaat ccgtcaatta ttttttcatc catcagcgaa
     7141 aatgcgaaac agcgctttcc caggtctttc agcgatgcgc caatgtggta aagtctttta
     7201 ttgtctatta gcaaaaagcg gtcatgggct gcgtcagaaa tttttatttc aacaggtgga
     7261 tattgtgttt gtcaatgtct gtttgcagcg cagcgctgac ttttttcaga taaatgtgtg
     7321 cagcaatatc cggcttcctt ttcgaaagca gcatcaatac cgatgcatcc acttagtggt
     7381 tccaaagttc aatcgatttt tcagtagaat gaatcaagtc agtcacaaac atccatgcat
     7441 cgaaaatctg tccttcgaag aagatgcctt gttttggcgc atcagcacgg gcttcgagcg
     7501 cattgaaaac tttttcgaat ttgaggtctg tttcgttttg tctgtattcc aaagtgttta
     7561 tcctcaaatt caaatcattg ttttgattcc gcatttttct catgtgtaca aaagcattca
     7621 taatacaaat gcttgtctga attgctactt tgctattgat tacagcggat agcatggcca
     7681 ctccctgctc caagaatgcg tgcggaagcg ctgatgaatg tttaagcatt tgaaaccggt
     7741 cgcaaattgc gaccagttgg ttcaattctt ctcttgagag tctgaaacag aaattctcag
     7801 gaaaccggga caaatttcgt ttcacttgtt cattgacacg ttttgttggg acctgataaa
     7861 gctcagccag atcgcggtca agcattaccg gaatgccacg tatggtaaaa atccggtttt
     7921 caatttccgg attaaccaat tctaaaccag tttgcatcat ttcgatgatt ttatgcagcc
     7981 agattcactg tcaattctgt agcgaggatg tacaccctgt tttctttctt tccgccgccg
     8041 atttctgttt cctggcttcg caggcggcct ttgagcgata catggcttcc gcacctgacc
     8101 atgtcaacaa tcttgcgggc agcatggtcc caggctacaa tgttatgcca gacagtcgtc
     8161 cggatccatt gtccgttgct gtcgcggaac aattgatgtg tgcccaatgt gaaactcgct
     8221 ttcagtctgt tttcgccata gcgctgaaat ttggggtctt ttcccgcata tccactcagc
     8281 tccacctgat tcgacggcca ttccttgttc tgcttttgtt ttttagatga attttccgct
     8341 ttcatactcg tttttttgca aatgtatttg cttcgttttg catgagccgg tattaagtat
     8401 ttactttcgg atataagtat ttgtaactgt ttgttgtcgg ctataacctc tttatcaaac
     8461 caatcatgta ttttctcgaa agaaaatcaa attaattatt tttggaaaaa ggattgtcgc
     8521 tgttcgtgct tcgcagcttt ctttgatgct ttcggccaca atttcgttat gcattgtgcg
     8581 ccgcatgcct caagccctgt cctaagccca gtgtctgagc accgacgctt cggttggcac
     8641 atattgctga tggctgaaaa ctgacaactg gcaactgata actggctact tgcaactatt
     8701 catacacaat cagaaagaca tcttcattgt cgcgtttcat gagatatttt tcgcgagcaa
     8761 atttctccat tcgttcatca ttacttacaa cttcgagata ggtttttgtg tcccggtgaa
     8821 tttcatcgag ataaaactgt ctttcttttc tgattttgtg gagttcgctg cgcagcgatt
     8881 gctggtgaat aaaattgttc tggtcaaaaa aaccgaccca cactacaaag acggtggtcg
     8941 caatgaaata tttattttta atgattgacc agattttttt cttcataata tgcaaaaata
     9001 aaaaaaactg cctgaacctg gaatggcgca gacagttttt atcaaacaac agaagttgat
     9061 atccttattt caggatagta acccagttgt gtttatcttc ttcgtctcca tattgaattc
     9121 ctgaaagttt tttgtaaaga gctgtgctcc attttccggc tttgccgtct ttgcagaatt
     9181 cgtatttggt gccagtttcg aggtcttcga tcagtccaat gggcgaaata acagcggcag
     9241 ttccgcaggc acccacttct tcgaaagaac caagttcgtc gagtgggatg gggcgacgtt
     9301 ctactttcag gcccaggtct tcggccagca cctgcagact cttgttggtg atggagggca
     9361 gaattgaaga gctgctgggc gttatatagg tattatcttt aattccgaag aaattggcag
     9421 caccgatttc gtcaataaac cttttttctt tggcatcgag gtacatgggt gaaccgtagc
     9481 cggctttgtg tgctttcata acaccggtga gactggctgc gtagttcccg cccactttga
     9541 tggtgccggt tcccagcgga gcagcacgat cactgtcttt aataatcgca atttttacag
     9601 gattgaatcc ttctttaaaa taaggaccta ccggggttac aaaaataatg aacatgtatt
     9661 cgctcgccgg ttttacgcct acttcggcac ctgagccaaa caacaacggg cggatgtaca
     9721 acgaagcacc ggttccgtaa ggaggcagat aatcgaggtt ttcgagaaca acagctttaa
     9781 tgcactcgaa aaacaggtct tcgggcactt cggccataag aattccctga gcactgcgtg
     9841 ccaggcgttt tgcgttttca tcccaacgga ataagcgtac atcgccattc tttccgcgat
     9901 acgccttcat tccctcaaag gcttcctgcc cataatgcag agccgttgcg gcaatgtgaa
     9961 tattcatata ttcagagtcc gttatttcgg gtttcgacca ggcgccgttt ttatagtagc
    10021 accggacgtt ttttgctgtc ttttgatatc caaaaggcaa ttttgaccat tctatatttt
    10081 ccatatcagt gtgttataga ttcgctgcta aagtacatat taattttaat taggtctgtt
    10141 tgtttccgtt tttttggatt gataaataat aggaagctgt ttgataattg gcactatatg
    10201 aagaattgct gatttctaaa acaaacttta aggaacagta ttaatattat ttttaatttt
    10261 gaaaaaaatt aatgtttgat tcgaaaaatc atgtataaat cgatgacact aagtatctct
    10321 ctattgttat tggttttatt gccggcaatc aaaatgcttg gtcaatccat tattaacttg
    10381 tacacaccgg taacagttaa ctctcctctt ttcagtaggg cgaatgaaat tcagtttggg
    10441 gccaatctga acaattttgg gctcaactat aaagtgaacg gacaatgggc caataccatc
    10501 atgattgcaa gtattcagca taatcccgga agcattgaat tcgatccttt gaatttcaac
    10561 gattattcgg atcagacgga tcagacacat ctgattcaat caagaccgga caggatgatg
    10621 tattgcgaac tcggccttgg atataatttg caccataaaa cacagaaaat ctgttttctg
    10681 gctggtgcag gccggcagat aattcatccg aatactcgat ggttctttca gtttgattgg
    10741 ggcaatgaga gcagtctgat caatgccgga attactgtga gggctaatta tacaaaagtg
    10801 aatggggtag acttcttcac aatggagccg atggtgcagg gaaagctgaa aatctggaat
    10861 ttcaggattg tcaatcagtt cgggtattcg gttgctatga agaaaaatga ggattatatg
    10921 aaaccagtgc ttaccgtcgg gctggagtat gttataggta aggcagcccg gaataaggga
    10981 tacaaggaat agtgatctgg gacgtgtgtg acgtctgcga cgagtcgcag ttgtccccag
    11041 cgtcccaaga gtgaggattt gccgcttaat tctcattttg ccagcgcaat agtcctcaag
    11101 gtgtttattt tgaattaata gtgtttttgt atttttgatt ttatatttcg attttgtcat
    11161 ctcatgctgt agctttttta gcaattaatt atgtccgatc agaaaaaaaa cactcagggc
    11221 tttattccca cgcatggtgg gtacaggaat cttttcagtt atcagaaggc agagataatt
    11281 tatgatggaa cagtttattt cactaatcgt ttttttagta aatatgaccg caccgtaggc
    11341 cagatgattc aggccgcgcg tagcgggaag caaaatattg ccgaaggaag tatggcttcg
    11401 gccacttcaa aagaaacaga gatcaagttg actaatgttg cgcgtgccag tctggaagag
    11461 ctccgtattg attatgaaga ttttttacgc acaaacaaac tccctgtctg ggacaagaac
    11521 catcgcctgg tagcccgtat tcgtgaactc aacaaaagta cgccgactcc tacttatgag
    11581 actttcagaa aggccattga acacgaaaat ccagagattt gcgccaatgc gatgattacg
    11641 ttaattcaga ttacaacctt tttactgaat cagcaaatca aacaactgga agctgcattc
    11701 cttaatgatg gcggcttgcg agaacgaatg actcacgcaa gattgcaaag aagaaacaac
    11761 aaagaataat ttttcgggac tgttgcgact gctgtgacaa tgttgcagta gtcccgagtg
    11821 tcccaggcgt ggaaaatagt agcatcattc tcactccacc agcgaaatat tcaccatcag
    11881 cttttccagt tttttcttga gtgcgaaagc gttgcctgaa taattgttca caatgatgca
    11941 aaaggccatc atttcatttt tctggttggg aacatatcct gcgtaggcgc gcaccgtttt
    12001 catgtatcca cttttggctc tgagcttacc tttcactggg gtgccctcga acatggtttt
    12061 caacgtcccg ctttcaccgg caatgggcag cgatgcgtac aatatttcaa accagggctc
    12121 tgtgtggata ttcataagga atgtgcccat ggctttcggc gaaacacggt tggctttcga
    12181 aagtccgctg ccatcgtgca attccattcc gtttttctga acgctgtttt cgccgagcag
    12241 gtcgtaaacc gctttcagtc cgctttccca ggaaccacta ccgtattttt tcagtccgga
    12301 cattttcagg caagtctctg cagcgacgtt gtagctttta ttgttggtgt attgaatgat
    12361 ttcggacaat ggctgcgaat aataggtcat aagcggtctg ccgtattctc cgttgtttag
    12421 ataatagcca cagcattttc ctgaaacgac gattcctttt tgcccgagaa atgattgcac
    12481 tgcaatagtg aaacccagcg ccggatcggg tgttgagcca tataccttaa agttgttttt
    12541 ccctggaggg agtgtgccgt tgatgatgcg ggtgaagtcg aacggatcgc cccagactat
    12601 gctgttgtcg ccggttccgc tgcggcctgc tgtggttttg ttttcgagaa caaattgctc
    12661 aaatgcagga aatgctgaaa cataagtcgt ttttgcacca aaagtggctg gcgtgttgaa
    12721 cgaaagcgtc agggcatttc catcccagat aaggcctgca gtgccggctc cgtagtagtt
    12781 gccgcggtca tcatcgggat atgtgtcagg aatgatcagt gaatcgaata ttccggcata
    12841 gccgttcaga tttccttcga tggctttaat gccgcttctg gtgagtgctg catacaatga
    12901 gtcaataaat ctctccttgg taaaaaacgg gaatccgatg gtggggtctg cgccaccgac
    12961 aatattcagg ttgccatgca aaacgctgtc agagccaatc tcgccgtcga tatacacatg
    13021 cgtgacaaag cggaatgtat ctccaagaac atcaagcgca gttgaagtcg aagtgagctt
    13081 tatgatggaa gcgggtatca ataaactgtc ggcgttgtat tcttcaatga ctttattctg
    13141 atcacagttg aatacaatta ctccccaatt gctttgtttc agatacggat cgttttttag
    13201 attttcgatt gactggtgca gcaaggtgtt ttgtgctccc atcggtagaa gacaagtatg
    13261 aagcagtaat atcagcagaa tttttttcat tgttcgctga ttttttttct gatcagatga
    13321 atgatttcaa ccaattgagt atcatttccg ctttcaagat cttctgaatt ttcaggcaat
    13381 acaatatcag gagtgatggg tgtgagtacg ggcggcgctg tatcctgatt gaagcggagt
    13441 atgctgatgg ttcctgtgat tccgctgttg ggcagttcga aaggagtggg ttgtccgaag
    13501 gttcctgaaa ttgcgcatcc ggcgggcaat cccgcaaaga gcgtattcgg acgtttgcgg
    13561 agtgtgttgc aaacaagacc agtggtagaa gcactcattc cattaaataa tacaaccaga
    13621 aagccggaat agtttttcgc ggattgaatg ggtttaactt gtttttccca tgtagtatca
    13681 acgatacctt ctgttttgtg ccatagagcc ctcatggctt tattgccgaa tgtacattgc
    13741 agcagtctga caatttcggg ttgaaacggc gtttgtcgga taatttcgtg ttgcactatg
    13801 ctgcttgatt ttgaaatcag atgattgaca taggtgtatg gctcttgcat cagaaagcgg
    13861 gtcagcagct taccgtagtc agcaaatccg ccaccgttgt gacgcaggtc aagaataaga
    13921 accttgggtt gtttggcgtt aatgtttctg aatgcattat taagaaaaaa gtgatattct
    13981 cccgggtttc cgttcatgaa tgatgctatt ctcatatatg caatggtgcg gtcttttgaa
    14041 tagccaaaag aaaaaggttt tatgctttca tttcccgcac ctgaaaagta gctgtgatat
    14101 ggttctttgt tggcatcgac accaggtaat aaacaggtat gtgtggtcga gtcagttcct
    14161 gtttgaatgg tatttattga gtcaacgaaa aaaaacaggg gatatacgga tgtgaaataa
    14221 tattctgcaa ttttttcacg actgtgaatg ttgtttcctt ctgaggatga caacatcatc
    14281 agtaccggat agattgagtc ggcagggata ttattgatgc tgaaaatttt gtcgccggtg
    14341 atgcatgagt cgaagtaatc tccgcgcaca tacagacttc cgttgctgat aaatacatcg
    14401 agcggcagaa atggcttgcc ggcctgcaga taatcattgc tttcgggtgg caggttcagt
    14461 gcacaatgaa tatcgcgtat cgagctgaaa acaggcatga gtcggcggta gagttctgtg
    14521 gcgctgatcg agtctgtgat tccggattgg agggtgtcaa tgcgatatcg cagcatggat
    14581 gtatcgcaaa ccagcgccag attgatgtgt gcatcgaaaa atatgttttc aaaaaaggaa
    14641 atatctgatt ttgcctgtgc aggcgaaagc aacggtagtt ccgtttgttg tgcttcagaa
    14701 gacaactgat tcagaaaaac gaaaatcaaa ataaatttca gcagattcat caatcgcaaa
    14761 gataaaaaaa tgtactcact ctgatgtatg ataaacgaat ttcggttgat ttgttatgtg
    14821 aaataagatt tttatatatt acatttgtat ggaaaaactg aatgatggaa atgctattat
    14881 ctcttttaca tactgcactt accttaacag gcattgtgct tgtgatgatg cttattattg
    14941 aatttttcaa tgttcgcagt cagggtaaat ggttgacccg aatgcaaaaa aatgtaacag
    15001 gacaaattct tctgggtaca tttatggggg ttttaccagg gtgtttcggt acttttttta
    15061 ttgtatcgct ttttactcat ggaaatgtcg gctttggggc tcttgtagct actttgattg
    15121 ccacatcagg cgatgaggct tttctgatgt tttcaatgtt tcccatgcaa acggttttta
    15181 ttcatattgg gttggctgtt gtggctatta ttgccggttt tgtggcgcat gccatattca
    15241 gaaaaaaaac catcatgacc cgtaaaatgc agtttgagat tcactcgcat gaagaaatac
    15301 catctgccag aaattctggc attcgtgata atctgaaaaa tatttctttt catcgtgcag
    15361 ttttgatttt tggtttgctg ctggttattg tcaatctggt tcttggcgca gcacatggac
    15421 atgggggtgg tcacgaaatg aaacattttc attttgaaga atacattaaa tatatttttg
    15481 ccggacttgc ctttattaca ttattggcgg catttatact gcccgatcat tttatcaatg
    15541 aacacatgtg ggggcatgtg attaaaaaac attttctgaa agttttttta tggacattcg
    15601 gggccctcat tgttattgaa tttgcacttc ccgaacttaa tgccgaaatg tggatgaagg
    15661 aaaatccgat tacgctttta ttgattgcat gtctgattgg aatcattcct gaatcggggc
    15721 cacatatggt gtttgtaact atgtttgccg gtggctacat tccgcttgga gtactgatgg
    15781 ggtcgagtat tgtgcaggat ggtcatggag ctttaccatt gctggccgaa tcgcagaaga
    15841 gttttcttat tgcaaaggga ataaacattt ttgtagggtt tgttgttggc tatgccttta
    15901 tattatttaa tattggattt tgaaaacatt gagcccaatc atttcttaag caaaactgga
    15961 tatgacggaa aatgaaaaat tggatagaat caagtttctg acgacagaac tgaacaggca
    16021 caactatttg tattattcaa aagaccggcc tgagatttcg gactatgatt ttgatatgct
    16081 gcttaaggaa ctggagcaac ttgaaaagga aacaggacat gttgaaccgg acagcccgac
    16141 gcagcgcgta ggcgggcagg taaccaaaca attcgacagt gctgatcata ttttcccgat
    16201 gctttcatta tcgaatgtat attccgttga agagctgaat gattttttta cgcgttgcat
    16261 caagtctggc ggcgatgatg ttgaatttac ctgcgaaccc aaatacgatg gcgttgctat
    16321 tagtttagtg tatgaaaacg gattgttggt gcgtgcggtt acgcgcggtg atggaagtcg
    16381 gggtgatgtg gttacagcca atgtgaaaac aattcgtagt attcctatgc gactgatggg
    16441 aagcgattat ccgcaattgt ttgaaatgcg tggtgaaatt attttacctt tctcttcgtt
    16501 tgaaagaatg aatcaggagc ggtttgaaaa tggtgatgag ccatttgcca atccacgaaa
    16561 cgctgcttcg ggcacattaa aacttcagga ttctgctgag gttgcggcac gtggacttga
    16621 ttgcatgctc tactcattta tcagtgatga agttgttgcc gatacgcatt ttgatgtgtt
    16681 gcaaaaagca aaatcatggg ggtttaaaat tcatgatttt attcgtcgct gcaggggtgt
    16741 cgatgctgtg aaatcatatc tcgatgaatg gtcggcaaaa cgcaatgagc ttccgtttgc
    16801 aattgatggt gctgttatca aagccaatga tattgcgctt caggaacaga tgggctacac
    16861 agccaaatcg ccccgctggg ctgttgctta taagtataaa gccgaagctg catgtacaaa
    16921 attgttatcg gttgaattct cggttggacg tacaggagtg gttacgcccg ttgcaaatct
    16981 tgatccggtg cagcttgcag gtactactgt gaaacgagct tcgttgcata atgccgatat
    17041 aatttcgcag ctcgatgtgc gtattggtga ttcggtgtat gtagagaaag gcggtgaaat
    17101 tattcctaaa atcacaggag ttgaattgtc gctgcgtccc gatgacagtg ttccatttga
    17161 atatgcaaag caatgtcccg aatgtggaac tccgctccaa cgcaatgaag gcgaagcggc
    17221 ctggttctgc ccgaacgata tggaatgtcc gccacagatc aaagggaaaa tcgagcattt
    17281 tatctcgcgc aaggccatga acattgatag tttgggcgaa gggaaagtag aacttttgta
    17341 cgataaagga ttgataaaaa atgtggctga tttgtattca ctccgagccg aacagttgct
    17401 tggccttgaa aaatccatga tggatgaaac cactggaaag acgcgtgtag tgcggtttca
    17461 ggaaaagagt gtcgaaaata ttctgaatgg aattgaaaat tcaaaatctg ttccgtttga
    17521 aaaagtttta tttgcattgg gcatccggca tgtgggtgaa acgcttgcag cgaagattgc
    17581 gcgccatgtt gaaagcatgg atgccttgat gaaaatgaag gccgaggagt tggtgctcat
    17641 tgaagatgtt ggagttgtta ttgccgaaag tattgtgcaa tggagtcaat caatcactca
    17701 tatgcagctt gtagagcggc taaagctgtc cgggctgcaa ttcgaagtga agaaacaggc
    17761 cttacagagc gaaaagttga aacaacttac atttgttatc agcggagttt tttcatctcc
    17821 tgaacgacgc aatgagttga agaaaattgt ggaagtcaat ggcggtaaag tggcatcgtc
    17881 ggtgtcggca aaaacaaatt atctgcttgc tggtgacaac atgggccctg ccaagcgtca
    17941 ggccgcagaa aaacttggag tcaagattat taacgaaggt gaatttttga tgatgatcgg
    18001 gtagaaagaa atgtctttta tttggtcgat tcccaatacg tactcccgtt tttggttaca
    18061 cgaaattccg gcttgagtcc tgttgcctgg ctgaattcgt gaatggcttc gtattcgatt
    18121 ggaagaacta attttccttt tttatcaatg acgccgcatt ttttattttg atatacaacc
    18181 acaaattgct gatcgtacca ggtgtggccg acaatcatgt catatataaa ttctgttacc
    18241 aatttgcctt tatcatttat gattgcccat ttttcttctt tctgaacggc ggtaacatca
    18301 atgatgaaat caaatacccg atcgtaaacc ggcgccacaa cgatctttcc tgtggtatca
    18361 ataaatccgt atttattatt ctcctgatag aaaacccgat tgttgtgaat ttcatagcca
    18421 agatgtttgt atttgcaagg aatgacttct tttccatgtc ggtcgatgta tccatataat
    18481 gaatcacgca tgaccattgc atatggattc ggatctctgg tgtacatgat ttgtcggaac
    18541 ccgttgaatt gttgatatcg cgcgggaatc aggatattgg tgtttaagtc catcagacca
    18601 aatagtccgt tctttttaaa aatcaggttg ccgctcagat cgaattgaat caattcatat
    18661 ttacagggaa taatgatttg aaaatttgaa tcaatcacgc catacatcgt atcccggcgt
    18721 atgagcgcaa tgtttttatc tttccagaaa tgacctattt cctcgtattt gcagggcaag
    18781 ataatttttc ctgatttatc agccagccca attaatttgt tgcggctgat acgataaaaa
    18841 tcttcattcg gcattttata caactgatcg taaatgaccg gtaaaactac gatgccttca
    18901 ttatttacga gaccatatac gtatccgcga ctgacaatat ttccatttgt tttttgccac
    18961 tgcacaacat gatcgcagct ctgaatacgg ttcccaagtg tatcaatgca aaacttttcg
    19021 tctttgaatt gcaccagagc ataatcatcg cgcccaaaat tttccgagtc ctcgaattga
    19081 catgggatta cttctttgcc ctgtttgttg atgtaacccc atttgccgtc gcggaaaacg
    19141 cggacaagtt ttaaatcgct ggcataaaaa ctttcacata tttcgtaaac cggcgccact
    19201 acttcggtgt acgatgaatc gataaaccca attcgcccgt cttttttcac cattgtcacc
    19261 ggatattgat gaatgtattc cggattaatg atttcatcgt aatcgccgat atcttgcacg
    19321 gtgcttttgt caatgcggta atgcttttta actttatcgt tttttgaaaa tgaaatcagt
    19381 tcctgacttt cccaaacata ggaatcatat tccatcggaa tgatttcttt ctgttcttta
    19441 ttcataactc cccatttgcc attcagccgg gtcagcagat tgtttttgag tggatacagc
    19501 gcatcgtatt tcatgggaat caccaggttg cccagcgaat caatcaatcc tgatttgctg
    19561 gtgccgtaag gattgtcatt gccttgaaaa tatgaatgta cttttgctgt tgtatcaatg
    19621 ttaatcaggt tatatacttt ataaaatccg ttgaaaaagt tcatcggctg cgggctgtag
    19681 ggccacaccc ctttcgaaaa agcaactttg ccggcatgag ttggtatgaa ctgatgtatc
    19741 aggttgttgc tttttttatc gagtggaata tctttgaaaa gcgcattgag cgattcaagc
    19801 gaccaaaaag tattatacgc tttgataatg gctccggtgc tgtcgcgaaa aaaatattca
    19861 ttgggcgggg gtggtggttc gagcatactg tacgacattg cgcggggctg cgattgttcc
    19921 agaaaaaata ttttgcggtc ccaataggca aggtgatcga cttcgatcat ctgaacgccg
    19981 ttgtaaaacg gatagtaaat agtatcaatc tgttccggca ttcgcgggcg gaaaatgtca
    20041 tcaatatcgc tctgcgcacg acacgacatt gttgttaggc cgacaatggc cataaagata
    20101 agtatccgca tctgattttg aataaggcgc ccttttgtac aaggttcgtg ccgggttgaa
    20161 aacttgttgt gtttttatgt tttcttttat ttataccttt gaaaaaaaat tacagcgatt
    20221 atgaatttcg atgtcattgt gatcgggagt ggtcccggag gatatgtagc agccatcagg
    20281 gcttcgcagc ttggaatgaa aacagccgta gtggaaaagg ccgaagtggg cggaatctgc
    20341 ctcaactggg gatgtatccc taccaaggcg ctgatgaaaa gtgcccaggc ctataaatac
    20401 atgaagcatg cggccgatta tggcattaag ctcgaaggtg aggtgaaacc tgattttccg
    20461 gcaatggtat cgcgcagccg cggtgttgcc gacaccatga gcaaaggcat tcagtttctt
    20521 ttcaaaaaaa ataaaattga agtcctgcct ggtttcggaa aactgatgcc cggtaaaaaa
    20581 gtggaagtta cgtcagccga cggaagcaaa accgtgtatc aggccaatca tatcatactg
    20641 gctaccggtg cgcgttcgcg ggaattgccc gcattgaaac aggatggtga aaaaattatt
    20701 ggctaccgcg aagccatgac attgccgcag cagcccgaaa gcatggtggt tgtgggtagt
    20761 ggtgccatcg gcagcgaatt tgcctatttc tacaatacca tcggaacaaa agtgacgctc
    20821 attgaattcc tgccgaacat tgttccagtc gaagacgaag aagtgagcaa acaactcgag
    20881 cgctcgttca agaaagcagg aatgaatatt ctcacttcag ctgaagtgac cagagtggat
    20941 aaagatggcg ataaatgcaa ggtgtacgtg aataccagaa agggcgaaga ggttttcgaa
    21001 gccgatgtgg tattgtctgc agttggcgtt gccactaatc tcgaaggtct tggacttgaa
    21061 gaaaccggtg ttgcatctga gcgtggcaaa gtgctggtgg atgattatta ccgcaccaat
    21121 gttgaaggtg tgtatgccat cggcgatatc gtaaaaggtc ctgctttggc gcatgtggct
    21181 tcgcatgaag gaattatctg tgtggagaaa attgccggca tggatgttca tccgctggat
    21241 tatggcaata tacctggctg tacctatact tttccggaag ttgcttcggt aggaatgact
    21301 gagaaagccg cattggaagc cggttttgag attaagaccg gaaagttccc gttcactgcc
    21361 agcggcaaag ccaaagcagc cggcaacacc gacggatttg tgaaagtgat atttgatgcg
    21421 aaaaccaatc agtggctggg tgcgcatatg attggcgaca atgtaacgga aatgattgcc
    21481 gaagcagttg ttgcacgcaa catgaaagtg accggacacg atattattaa atcagtacat
    21541 ccgcacccaa ccatgagcga agctgtgatg gaagctgtgg cagcggcata cggcgaagtg
    21601 attcatttgt agttctcatt cccgcaaccc accaaaattt atgtgcggaa taacaggtgt
    21661 tttttcaaaa aacgataatg gccggaaata ttttgaaagt gttccggttg cattagaaac
    21721 gctcaggcag cggggaccgg attattctgc caccaaaggc ggcgatcatt ttttatttgg
    21781 tcacgcgcgt ctgtcgatca tcgataccag cgaagcatcg catcagccct ttgtttcgga
    21841 ggatgaccgc tataccatgg ttttcaatgg agagattttc aattttcgtg aaatccgcac
    21901 agagcttgag aagcagggac atacttttcg caccagtggt gatgtggaag ttgttttgaa
    21961 atcgttcatt caatatgacg taaagtgttt cgaaaccttc aacggatttt tcgccatagg
    22021 catctttgat tcatgggaac agcgactggt gcttgcccgc gaccgtttcg gcgtgaaacc
    22081 attgttgtat tattccgaag catcggtatt tgtttttgct tcggaaatga aagccattct
    22141 gaaatatccg attcgtcgga cacttgatga acagagcgtt tacgagtatt tcagactgac
    22201 gtatattccg gaaccggatt ctgtttttca ggaagtgaaa aagttgcagc ctgctcatta
    22261 tgcggtaata acaagggata aaacggagtt taatgcctat tattccattg cggatgaagg
    22321 attttcaggt tcctacgaag atgcctgcga ccggctcata gagcttcttt cagacagtgt
    22381 tcgtatgcga ttgatttccg atgtgtcgat tggagctttt ttgagtggag gtattgattc
    22441 atcaataatt tccgtgttgg ctgcacaaca caatccgaac atacatgcat attccattgg
    22501 atttccggat gctccatatt acgatgaatc ggtttatgcg tcttctgttg cgaaaaagca
    22561 tggtttgcgg catacgatta ttccggtcac tgagtccgat tttttgaatg ctttgccatc
    22621 ggttcttgat tatattgatg agccctttgc tgattcgtcg gccattgcag tgaatgtgtt
    22681 gtcgtcgttt gtgcgaaagc atgtaacggt tgcattatcg ggcgatggtg ccgatgaagt
    22741 ttttggcgga tatcgcaaac accgcgcgct ggctatgatt gcacgtgcaa accgtgcgaa
    22801 gatgatggct tatacggctg cggccgccat tcccggcgta ttcggccgtg ggagccggtt
    22861 ttcagataaa atgcgtcagg cacgccgcat gggcagggtg gcatcgatgc ggcccgaaga
    22921 gcgttatctg tttctggctg cttttcattc aggccgcgaa gtatcgcatt tgttgcattt
    22981 gtcgaggcat tacagcatgg atcgtgtgcg gaaccaactt gatatttcac atccttctga
    23041 tatgaattca gtactcagaa acgatgttaa gcttgttctt gccggcgata tgctgcgaaa
    23101 agccgatttg atgtcgatgg caacgtcgct cgaaatacgt tcaccttttc ttgatcaccg
    23161 gataattgac tttgcctttt cgttgccatc taaatggaaa tttgattcct tgagtacaaa
    23221 aaagattctg acagattcgt atagggatat attacccgaa aatgttatca acagacctaa
    23281 acatggtttc gaagtaccgg tgggtaagtg gatgaatggt gctttattga atgaaataca
    23341 aacgaactgg ttcgaggata aatttatacg atatcaggga atttttcagt ctgaaaaaat
    23401 tcaacgttta aaacatcagg ttgaacgtgg aagggggctg agggatcaga gcttattgtg
    23461 gtcgcttatt gtgtttcaga gttggtggaa aaaatttatg taatggcaga aaaaatacgt
    23521 gtattaagaa tattgaatcg attcaatctg ggtggaccga catataatgc atcgcagctg
    23581 actaagcatc tggagccgga tttcgagacc ctgctgattg gtggagccaa tgaaaaagac
    23641 gaacatgatt cattgcatat tccgaattcg ctgggaatag agccggtcat tattgattca
    23701 atgcggcgtt caatgaatct tgtgttggat cgggctgcct atcgtgatat caaaaaaatc
    23761 atcaaggaat tcaaccccca tattgttcat acgcatgcat ccaaagccgg tgctgttggc
    23821 cgaagtgcag cgtttaacat gaaagttcct gtgattgttc acacctatca tggtcatgtt
    23881 tttgaagcat atttcaacgg actcatcagc tcggtttaca aatccattga gcgtaatctg
    23941 gcgcgaaaga ctgatgctat cattgcattg agtgaacggc agaaagcaga tctgacaagc
    24001 agattcagag tttgcgagaa agatcaggtg catgttattg ccaatggttt tgaccttgca
    24061 aaatttcagg aagacaaaaa aataaagcgg gcacagtttc gcgaaaaata caagctcaga
    24121 aatgatgaat ttgccattgg tataatcggc cgcatggtgc cggtgaaaaa ccacgccatg
    24181 tttgtcgatg ttgttgagcg cgtcaacaag ctctatcgcg gaaaagtgcg ttatttcatt
    24241 gtcggtgatg gcgaaacctg cgaagacacc cgccgccgat tcattcagaa acgcattgcc
    24301 atcaatgaca gcacagagcc ggacacaatg gttcatttca cctcgtggca gaaaaatgtg
    24361 gatgatatca tggccggatt ggatttgctt gtacttacat cgctgaatga aggaacgccg
    24421 gtttgtctta tcgaagcgca ggcatccaat tgtccggttg tgagcaccga tgtcggcggt
    24481 atccgcgaca tagtgattga cggcaaaacc gctctgctct cgccgtcgca ggatgttgaa
    24541 accatgagcc gcaatattgt gaaactcatc accgacaaga aaatgtatga atcgatgacg
    24601 cttcacggat gggaatttgt gcgggaccgc tttcagtatc agaggatggt ggaggatatg
    24661 aaggctttgt acatgaaact gcttgctcag caaaaagttc ggttgtaaat tgttttttga
    24721 acgaatagat aaggacattt attccttcag aaattttccc gtaaggattt gctctttatt
    24781 ttgtgcacgg ataatgtaaa ggcctgtatt taatgctgaa acattcagcg tgttttctcc
    24841 attggaaaga aattgttcaa gttgacgttt tccatttaaa tcaaaaaccg aaaccacaaa
    24901 atcgtcgttt tcgggtaact ttaaaaatag ctgatccgat gccggatttg gatatgccac
    24961 caaaggcgcc gcctcactgc ccgacatttc ttcgaaacca agaccggtaa tgttgatttg
    25021 tgataatgca acaggatagt agttgtaaac tttcactgtg tcggtatcgc caaaaccaga
    25081 taaataaata taactgccgt cgaaactcat gagcatttta tcggaattga aatacaagtt
    25141 atgcacatta gtgctttccg gaaaatcgta attccaaatg ggcacaaggt tttcaggatt
    25201 cagtagaaca agattttcgc tgaaatggtg tatgagattt gattccagtg agtcgccatt
    25261 tttgtaataa aacataccgg ccagagtgtc atattcaaat gataaatttt ctttgccccc
    25321 taagccggga taattgtcca ccaatgtatc atagccatta taaaattccg tgataaaaat
    25381 tctgttttca ttttcgataa atttcgaatt gtttataacg ggaattgaaa agtaagaata
    25441 atacatggag ttgaaattat tatcaagttt attgatgagg ccgtaattcc aataaacaaa
    25501 actatctatt gaagcaacaa acactttttt aggaataatg gagtctggat tcataaaaac
    25561 aggataatag aatcttccca gagtgaagaa gttcccgctc tcgtcaatgg caaaatcatt
    25621 atattctccc ggtaatttat gattttgtaa aacaaacaaa ttcctgtcaa actcaagcgc
    25681 aaatgtgtgt aatccggcag ttgtatcagg cgtaaaaagc atattgtcaa ctatgaccga
    25741 atcgctaata ctgcccagca catacaattt atcactgtgc aatttggaat caattgggaa
    25801 atcataattt ctgtttttaa gcaaaacggg aataacctta ttgctaaccg aaagattaga
    25861 atcatatttc acaataaaac ctgcggtatc aagcggaaat gcacctgcag atccacttct
    25921 aagacaataa cataaatgta caaacacctc ttgatggctg tttactaaca ggttcttaat
    25981 aaataatgcg tagtttgaag cggcttcgct taaattggca agatccttta tggccggtgg
    26041 caaagccaga ctatcgtaac cgagaaaatt tccggaaagt gaataattta tcactaaaaa
    26101 tccgtttgtt tttggaaccg gaattccttt tcttagaatt atattatcgc gataggtggc
    26161 ggaatcgaga accggataat tgataatcac tctgaatgtg tcattctgaa taaaggttgt
    26221 agcaatattc tggttaagga aaatggaatc gagatatagt gtatcggcca tgacgatctg
    26281 attatcttgg ctgagtatgg ttggataagt ataacctgat ttaaaaatat ttggccacac
    26341 ccagttgtct tcatacggaa aaatggtgta tgcgaaatag taattctgat ttttcagagt
    26401 tatattgttt gtcgtaaata cagcctgatt ataaacagaa tcaacaacat catccgaaag
    26461 ccgcttccat tcaaacgttt ggctgaagga aacaatacaa agtattgtca atagtatggt
    26521 tgaaattatt cttttcatag ttgttgtttt tttagtgtga aacgatcagt ttttttgtta
    26581 gagtgttgtt ttggtcaata tattgaacca gataaatccc gtttggatag tttattgaat
    26641 ttattttcat tgtttgagcg ccatttataa attgaaattg gtcaataacg cgactattta
    26701 tatcggatat ccggatttct cctgattcgg aggcttgttg aaaaacaata gtaaaatcat
    26761 tattagccgg atttggataa acggaaaagt ctgtattgtt tttcatttca gaatatcctt
    26821 ccgtgtctta ataatgcccg taaacactta gtcctatgtg catggtttcg ccaatgtcca
    26881 gcggagcata atatccgaga ttataaacat atccggttgt ttttgccgtt aatccgtcaa
    26941 aataagtgag cattacactg tcttcaggag taaacagcca gtttgaaccc gattggtgtg
    27001 gttgtcccca taagtttgaa ctgctgttgc gaacccaaag agctgtataa aacggtggaa
    27061 taggtactct tttcccggta cggctgattg aaacagtgtc atattctgtc cggtgcaaaa
    27121 atatcttttt tgcttctgca tggacaaaat ctttcaaaat aatagttggt attgaatcta
    27181 taaaagccgg agtaacaaaa tccccgaaag gccttacaat attgcttgta tcgtttattg
    27241 tagccgggtt cacataacct gtatcaatcc ataaaagaag aacacttttt tgtactggtt
    27301 taccggcaat ggtatcataa aaatccggag ctgaaatttt tgcgttattc aattcgtaat
    27361 tgttattttg gctaaatgcc ggattcgaaa aaacacaact caccactatg ttcataaaaa
    27421 tgacgtagat ttttaaatcg tctaaaaaat gattacgtct ttttgtagat aaatgggggg
    27481 ggcggttcga cttatcgagc atgtttatag aaatatagat ttgattcatg aggttttttc
    27541 aggatcagac aagcaaagat aacaataatt tacttaaagt caagatatag tcaatgtatt
    27601 ttgtgctgaa aactaagaaa attcagacaa ggttttatat taaacatatt tttcatgaag
    27661 ctaatataaa cggtttttgt gatgccatct tgttacggca ggtaaccaat atgccatttc
    27721 atcgtaaaaa tggtcaattg tttaaaattg cctacctttg actattgttt gcactcttat
    27781 gcccaaaaaa atatctgcct taattctttt attgtcagtc gtttttgtat catgcaaccg
    27841 gaatttttat ccgtcagaca tgttcaaggt aaaggaccgg gcgagctttg ctcctgcttc
    27901 ggaactgttg gtgccggtta agccgctttt gcagaccgga gataatctgt caattaatgt
    27961 atattccaac aagggagaaa aactgctgga gccattgagt accgcatctc agcagggagc
    28021 aggtgagagc tcaccttttg tgtttacggt tgattcagcc gggaatattg agctgccggt
    28081 tgtcggacag atgcatgtgg ccgggcttac gattgaccag accgagaaag agattgcagc
    28141 cctttttgcc aactctgtgg ttgaacctta tgttcagatt tcggttctca ataaacggat
    28201 atttttcatg actggtggtg caagtcaggc agcaactgtt attccatatg tgaatgaaaa
    28261 cacaacactg atagatgtga ttaccgctgc cggcggcatc agagaaggga aggcctattc
    28321 catccgcttg ataagaaaca cgactgctgg attaaagatt tacgacattg atctttccga
    28381 ttttgcacag gcgaaatttt cttttgtgca ggtgctgccc aatgatattg tatatgtgga
    28441 accgcagcac aggccgttca gaaagtttgt tgaaagtctg gctccatatc ttaccctggt
    28501 ttccacaggt ttgctgattt ataacattac acgttgatac gaccatgacc gatagccgca
    28561 aagtaaaatt caacgatgaa ttcgatttca aactgttcct caatataacg aagaagagtc
    28621 tgaaatacat tattgttttt accattcttt tgtttgctgc tgttttcgtt tatttgcgtt
    28681 atacacagcc ggtttatgaa gctgcatgca ttgtgcagat caacaccgac gacaaggccg
    28741 aaaagctttt tgacaacaaa acgtattacg aggatgatat ttaccggaag attgaaatta
    28801 tgcggtcgcc ggtttttatg cagcgtgtcg tttctcagct tccgttggga gtgagcgttt
    28861 ttgcgaaggg aaacatcctc aattttgagt tatatgatga tatccctttt gatataaaat
    28921 atgaaattat tgattcaacc ttttatggtg ttccgttgct tgttgaagtt catgattccg
    28981 attttgttat agccgaaatc aatggagttg ttactgatac acgttttcat ttccacgagc
    29041 cggtttcgtt gcctcaggtg aattttgttt taacaccttt gcaaaacaac agccgtgcat
    29101 ttttcaatac caacaaaaac aagcagttca ttatacgctt caataatctc aatgatattg
    29161 cagctcagta ttcgcaaaag gtgaatattg ctgttattaa tgatgtggca aaaacaattc
    29221 gcatcagttt aaaagatttt aaccccagga aggcagctga tatcgtaaac agtgttgcaa
    29281 aggaatttgc tgcattcgaa gtggaatatc agaaaaacag ttcttcgagt attctggaat
    29341 ttattgattc gcagttgggt caggttgaag aaaatcttga tgaaacaaaa gattctcttc
    29401 aacaaacatc atcaccggcc attgattcta cacttgatcg taccgcactt cagtcgcggc
    29461 ttttcgagct tgacaatctg attttggaaa attcggccga ggttgaagcc ctcgaagttt
    29521 tttacaaggt gttttcgtcg ggaaatgccg atataattac attgttgcca ctcattagcg
    29581 gtattgaatc tcaggtatat ctgtcaccat atcttcagtc gcttaaagat cttgaaatgc
    29641 gcaagcagga attgctgctt cgtgttactg agaattcgac tgccatagag agtatcaatg
    29701 ctcagattga ttttcagaat aaaatcatca gagagagtac cagtggtttg ttgtcgaaat
    29761 acaaaaggca gcaacaacga tatacttctg aaaaaaacaa tattctgcgt ttactgggga
    29821 aacccattgt tggaacaatg gatgatcaaa gccgcattga gcgctattat tcagtgaatg
    29881 aaaaattcta caatcaactg attgaaaaga aaattgaata cagcattgct caggcagggt
    29941 atgttagtca gctgattgtt ttgcaacccg cacaatcgag ttttacgccg gtgtctccca
    30001 acaataaact tatctggatt ggggcatttg tttttgcgct cgtgatgggt tttgcctttc
    30061 ttgttttgcg ctatatactt tacaatgaag tggtgtcatt gcgtgatgtg aaaaaactta
    30121 ctacggttcc ggttctgggt gttatcccaa aatacaaaag ctacatcccc gtcagtcagt
    30181 tgattgtgga taccagaccc cgatccttgc ttgccgaagc gctcagggcc atacgaacca
    30241 atctgcagtt tataaaaaat attacgcagt cgaaagttat tgctatttcg agtactgtct
    30301 cgggagaagg aaagaccttt gtggccatta atttcgcagg tgttcttgcg tttaataaca
    30361 agaatgtgat tgtgcttgat tttgatttgc gaaaaccgaa aattcatctt ggtttcaata
    30421 ctgataatga aaaaggcatt tcttccattc ttttaaagat taatacacct gatgaatgta
    30481 ttcgccacag tagtattccc aatctgcatt ttataacggc tggtccaacg cctattaatc
    30541 cttcagagct tattttgacc aatgaaacgg atgatctgat tgcatatcta aagactaaat
    30601 atgattatat tatcatggat acgcctccaa tcggattggt gagcgatgcc atgaagccat
    30661 tgcaatatgc cgattatccg atttatgtta ttcgggcaaa ttattcaaaa cgttcttata
    30721 tttataatat tgaaaaattg tatcatgaga acaatctgag atttatgagt attatcctca
    30781 acaatgttga cccggagatt accggtgccg gcagttcaca gggttttgcc tatggttatg
    30841 gttacggata tggttataat aattcgcgac atggctatta tgattcagat gattcggttg
    30901 ccaaaacatg gcgcgaaaaa atattggata agtttagaag aaagcgatga tggaagtttt
    30961 aagatttgaa tgtgacgggt tgattctgat aaaaccgaaa gtgtatgccg atgaccgcgg
    31021 acattttctc gaaacctatc attggcagaa ataccgcgag ctgggaatta acagtgattt
    31081 tgtgcaggat aatgaaagtt tatcacaccg gcttgttttg cgcggcttgc attttcaatc
    31141 accgcctttt gcccagggaa aacttattag ggtacttcgg ggcagcatct atgatgtggc
    31201 agtcgatatc agaaaagatt cactcacctt cggaaaatgg caatcggttg ttctttcagc
    31261 cgaaaacaag caccagttct ggattccgga gggcttcgcc catggatttc tggcgctcga
    31321 ggataacaca gtaattaatt ataaatgtac agccaattat aatgcatcag cggaacgcac
    31381 attgctctgg aatgacactg atctgaacat cggatggccc gaaacgcccc tgattgttag
    31441 ttcaaaggat aaggcggggg agattttccg cgatttcgta tcaccatttt aatcgcttta
    31501 tgtatggtct ggaatgatat tgtacttgtg gtagttttgt tttttggatt ttctttgata
    31561 atcaacggct tatttctgaa attctccagt acacttggtc tgcgaacagc aaaggaagcg
    31621 ggtgtgcggt ggagtgcatc ccaaaagcca tcattcggag gcattacttt ttatatcctg
    31681 tttctgatat ccgttatttc ctattcaatg ttgtttccca atgcagccat ggttcgcaac
    31741 gtcgaatttc ttggattgat gctggccgta tccattggtt tcattgccgg attattcgat
    31801 gatgccttta ataccaaacc gcttattaaa ttcggattac agatattgac agcggtggta
    31861 attatttttt cgggatcagc aatctgtata accggaaaca tgtacggcga tatgattctt
    31921 actgctgtgt gggtcattgg aattatgaat gccattaatc tgcttgataa catggacgct
    31981 attgcttcgg tcgttgttat ttttatactg gcctgttttc ttgttattat gcaattgaac
    32041 gggttgaacg atggcccgta tctgtttatc tcaatcggat tgattgccgc tgttggtggt
    32101 tttttgtttt ttaactggaa tccttcgaaa ctttttatgg gtgacaccgg gagcatgttt
    32161 ttggggacag ctgttgcttt tcttggaatc aaatatggtt ggaatataca gcttgaaaac
    32221 gctgttgttg ccgattggca gaaagttctt actgttgttg ttgtttttat tgttccatta
    32281 accgatacta cgaccgtttt catcaagcga atcttttccg gacactctcc ttttattggc
    32341 ggcaaggatc atacaacaca tcatttgtct tatctcggac ttagtgagag agcagttgca
    32401 atggcttatt gcctcatcgg gcttctttct ttgctggcaa tcgtaaaatt attatctttg
    32461 ttcagtcatt ggaatggatt tctaaccctt gcctatgtct cttatattgt cattgtattc
    32521 gggggtctct tctatattgc tcatctgaat cgtgaccggg aataagccag atgcagtgtc
    32581 tttttcattg attacaggag gtcttttcaa aagactgttt ctgctttttt tgtctcatga
    32641 gcgcatcttt tttaaatact tattgtatat ccggaattat gtttgtgaaa aagtggatta
    32701 tcggccttat tgttatgctt attcttgcta ttgggggaat gcagttgttt atgttttcgg
    32761 cggtcaacag caatccccgg aatcctgatg agcaatattt tagcatgagc agacaaaccg
    32821 ttaaggtttt tcagccatat attccggatt cactccattt ctgcggcgag aaggtgccaa
    32881 ccgatcacct gattgtgcgc gaaagactgg aacgtgaaat tatggccaac atgtattggc
    32941 attcgcatac aattttgctc ctgaaacgat cgggtcgcta tttcccgctt attgagccca
    33001 ttttgaaaga aaatggagtg cacgacgata ttaaatttct tgcaatgtgt gaaagcgaac
    33061 ttaccaatgc tgtttcgcct gcaggagcaa aggggttctg gcagtttttg cataagacag
    33121 gacttgagta cgggcttgaa ataaacagca gcattgatga aagaaataat cttgaaaaat
    33181 cgacagtggc tgcctgcaaa tacatcaaat cgtcgctcag ccgtttcggt tcttatagtc
    33241 tcgctgctgc ttcgtataat atgggtagtg gcggattgca gcaaaaaatg aatgagcaga
    33301 aatgcaatga ttattattca ttgtttttga atgaggaaac ttcgcgctat gtttatcgca
    33361 ttcttgcgtt gaaattaatt tataacaacc cattgacgta tggttttttc atacgaaata
    33421 aagatattta ccgaccaata aaagttaagc acattgaggt ctcagcaagc atccccgatc
    33481 tggcagtctg gtcaaaagac aaaggggtct catatcttga actacgttat ctgaatccct
    33541 ggattatatc gaacagtctt aatgtgagtg ccggaaaaac atacaggatt gcattgccgg
    33601 tatcaatgag ctattcggaa agcatcgagg aaatcgggtc tccttttaca atttttcagg
    33661 attcagtgag tgtggagtag atgatgagta aagatgattt tttaaaggat tccgggcagg
    33721 agtggctcga agtgattgga gcgcgtgagc ataatctgaa agatgtttcg gtcagattac
    33781 cccgttacag tctggtggta attaccgggc tgagcggcag cggaaaatcg tcactggcct
    33841 tcgataccat atttgctgaa ggacaacggc gatatatgga tactttttct gcctatgcgc
    33901 gtcaatttat tggcgatatg tcgcgccccg atgtggacag aattaccgga ttgtcgcctg
    33961 ttatttcaat tgaccagaaa acttcgggtc gaaatccccg ttccacagtt ggaaccatca
    34021 ccgaaatcta tgactatctg cgtttgttgt atgcgcgggc atcaacagca tattccatgc
    34081 ataccggaaa acccatggtg cagtattccg atgcgcggat tgccgaaatg atcgggcagc
    34141 gttttgaagg tcgtcagatt gctgttcttg ctcctcttgt caaagcccga aaaggacatt
    34201 atcgcgagtt gtttgaacaa taccgtaaat taggatttgt tttttttcgt atcgatggtg
    34261 aaatcactga agttgcgcag ggattgcagg ttgaccgtta caaaattcat gatattgagt
    34321 tggtggttga ccgtattgaa atgtcgggta caagcaacaa acgattgatt aaggcgctgg
    34381 atctggcttt gcgctatggc aaaggcgtgg ttgccattca tgaggagcaa agtgttaata
    34441 ctgctttctt cagtcgtaaa ctgatgtgtg aagataccgg tgaatcgtat gaattgcccg
    34501 aacccaactc tttttcattc aacagtcctt atggggcatg tccccattgc aaagggctcg
    34561 gaacagtttc cgaagtggat atgaaaaaga ttattccgga taaaagggtt gtcattcgca
    34621 aaggtggaat agcgccgctc ggagagtata aaaacaactg ggtattcaaa caggtggaac
    34681 tgatatgcaa aaagtatggt cataccctcg atgatgaagt tggcgacttg agcgatgatg
    34741 cgcttaatgc tattttatac ggatcaaacg aaatgcttca tcttaccaat gattatctcg
    34801 gtgttacaag cagctacaat ttgtcttttg atggcattat caatatgctt gtcaaccggc
    34861 aggatgatga tatgaatccc gctctccgaa gatgggcaga gtcttttatg aatatgattc
    34921 catgtccaga atgtggtgga acacgtttaa aaaaagaatc gctccatttt cgcattgatg
    34981 gtaaaaatat tgcggaggtg tcggatttcg aaatggatca tttgctggag tgggtaggtc
    35041 atttgcctca gaagatggaa aagcgccatg ctgccattgc cgtggatatc attcgcgaac
    35101 ttacttcgag gattggtttt ttatccaagg ttggattgaa ttatctgagt ctgaaccgcc
    35161 cggcatcgtc gctttcggga ggcgagtctc agcgtattcg tcttgcttct cagattggat
    35221 caaagcttac cggggttttg tatattctgg atgaacccag catcggattg catcaaagtg
    35281 ataaccgtaa gctgattgag agtatcaggc gcttgcgcga cgccgggaat tccgttattg
    35341 tggttgaaca cgatgaagag atgattcgtt cagctgatta tgtgatagat atgggaccag
    35401 gagccggccg caaaggtgga tttattactt ttgccggtgt gccggccgac atggacaatc
    35461 acactactat tacctgcgat tatatcaatg gggtgaagtc aattcctgtc ccggaaattc
    35521 gacgtccaat agactccaga cgtatttcgg tcaagggtgc cgaagggcat aatctgaaaa
    35581 atcttcaggt ggattttcca cttggactgt ttgtgtgtat tacaggtgtt tcgggtagtg
    35641 ggaaaagctc gctggtgaat gaaaccttat acccggtatt gagcaataag ttatatcgca
    35701 gcaatgcacg ccctttgaaa tataaatcga tagagggatt ggaccatatc gataaagtca
    35761 ttgaaattga tcaggcaccc atcggaaaaa cgccaaggag caatccggct acctataccg
    35821 gtgtgtttga tgaaatcaga aagttattct cggttcttcc cgaatccaaa gcgcgtggtt
    35881 acaagccggg gcggttttct ttcaatgttg ctggtggccg ttgtgaaaca tgtaagggcg
    35941 ctggtctgaa aaccattgaa atgaattttc tccccaatgt ttatgtcaat tgcgatgatt
    36001 gtggcggaaa aaggtataac cgcgaaacac tagaagtgcg ttttaaggga aaaagcatca
    36061 gcgatgtgtt ggatatgacc attaatcagg catcagagtt tttcgaaaat cagtcatcga
    36121 ttctcaccta tgtgtcaaca ttacagaaag tcggactggg ctatataaaa cttggacaaa
    36181 gttcaaccac gctttcggga ggcgaagccc aacgcattaa acttgccact gagcttacgc
    36241 ggagagatac cggccgcacg ttatatattc tggatgaacc tacaaccggt cttcatttcg
    36301 aggatgtgcg tgttttgctc gaagtgctca acacacttgt taaaagagga aacactgttc
    36361 ttgttattga gcatcatctc gatgtgattg cctatgccga ttatatcatc gatctgggcc
    36421 ccggcggagg caagcatggt ggcaggattg ttgcgaaagg aactcccgaa gaagtgtcca
    36481 atgtaaacga tagtttaacg gggcaatatc tgaaggaaca ttttgaacaa agaaaaaaaa
    36541 taaaaaagta attttaccgt aatatgatga acgacaacaa cgggtttgaa cacctgaacg
    36601 atgatgaaaa aatccgccgc gcgctggagc ccaaaaactg gcatgaagta aagacgcatg
    36661 actcctggca gattttcaag attatgtccg aatttgtgga aggatttgaa acactggcta
    36721 ccattggtcc ctgtgttacc atttttggct cagcccgcac cctgacaggt gacccgtatt
    36781 acaaactggc tgttgacatt gcctataagc tttgtaaagc tggttatgga gttatcactg
    36841 gtggcggacc cggaattatg gaggccggaa atctcggtgc caaaaaagcc ggcggtaagt
    36901 cggtgggact gaacattcaa ttgccttttg agcaggaagc caatgagtat atcgacaaag
    36961 ataaaatatt gaattttaag tatttctttg ttcgtaaagt aatgtttatg aaatacagtc
    37021 agggttttat tgtattgcct ggcggttttg gcacctgcga tgaaatgttt gaagcgttga
    37081 cattgattca gaccaagaaa atagcgcatt tcccgattgt tttggttgga aaggaatact
    37141 ggaccgacct ggataagtgg tttaaagata cgatgctgaa atttggcaat atcgctgaaa
    37201 aagatctgga tctgtatagt tttgccgaaa cgcccgaaga ggccgtagct gttatcgaaa
    37261 attattacag gaaatacaac ttgtcgccaa acttctgata atgaagtcaa tactttcgtt
    37321 gcgttttttg acggcgatgg aaggtcagca atcgcacatt gctgttgatg cagtaatcaa
    37381 tggcaaaccc gctttgatga tcatagacac cggtgcatca aattgtgtgc ttgattcagc
    37441 gagggctgta aaattcgaac tgcttcatga ccctttcttt ataagtgatt cggccattgg
    37501 tattggttcc gggcaattga gtgtaacact ctcgagaagc gaatactttg agctgggtac
    37561 atttcgtatc gaattgtttc cttttgtttt acttgatctt gcaaccatca atgcaacttt
    37621 tgccaaagcc ggatgcgagc cggtggatgg cattatcgga acggatcttt tgctggcggc
    37681 caatgctgta attaattata aatcagccac cataactttc aggggtaaca aaaaggtctt
    37741 gaaaaagttg tttcagaaca tatcgctttc ataatggaga atggtgataa tgaggtaaat
    37801 gcgaatttct taaattattt tagggtaaaa tagtatataa ttcaatcagt tattctatct
    37861 ttgcaccctc aaaattttga cgaaaagtct tttaaactgt ttgatatgca aataaaagga
    37921 acgatcaggt tttttgctgt tattttcatt ctggtaagtg tgtattccct ctctttcacc
    37981 tattgcacac gcaaggtgga aaaagaggca aaagaatatg ctaccagcga agccgttttg
    38041 cagctgtcca aagaaattgc ggaaggtgat tcacttcgtg aaagagttgt tttcgactcc
    38101 atttctgctg cctgggaaag ttattatctc gactcaatgt cgaatgaagt catttacaat
    38161 ctgcttgtta agaaatatac ctacaaggat tgcaaggaaa aagagctcaa tcttggtctc
    38221 gaccttaagg gtggtatgaa cgttactctc gaaacttcgg aagcggatat tattactgca
    38281 ctttcgggta acaatcagga tccgaacttc aaaaaggcca ttaaaaatgc ttcagagaaa
    38341 cagaaaaatt ctcaatcggc ttatgttgac ttatttgtag aaagcatcag agagcttgcg
    38401 ccgggtaagc ccctggcttc attttttctg acacgtgaac tcagtgagcg tatcaaaaca
    38461 aatagcagcg atgatgaggt aatcaccgtt ttgaacgaag aaatcagcag cgccatcgat
    38521 cgcactttca tggtgcttcg caaacgtatt gataaatttg gtgttacaca gccgaatatt
    38581 cagaaactca gcaccagcgg acgtattctt gttgaactgc ctggtgtgaa ggatcctacc
    38641 cgtgttcgta aaattctcca gggaactgca aaactggaat tctgggaaac ctacgatttt
    38701 gttgaaatct ggccggcgct tgaaaaagcc gatcagacca tcaaaaatat gatcagcaaa
    38761 cagcttgttt cggttgattc ctctgtaact gataccattg ttgctgccga tacaacagtt
    38821 gctactgctc aaacacaatc aaatactctg gaagaaaaac ttgaccagat tccgggtcag
    38881 accagtacta ctcaggattc atcagatgta aaatcccaga gcccgctgat ttctctgttt
    38941 gccagtttca atattgttga aagtgaaggt aaacgttttg ccggtgaagg tccactgatc
    39001 ggatatgtac attcaaagga tacagctcag gtgaacgctt atctgagaat ggttcagagt
    39061 tcatttacaa atactacttt tgcctggggt gcaaaacctg ttccaatgga tattccgggg
    39121 actttttatg aattgtatgc ccttaagata agcacaacgg atggccgtcc aaaacttgaa
    39181 ggagataaag ttgctgatgc ctggcaggat tacgaccaga atggccgtgt ggaagtttcc
    39241 atgatgatga ccagcgaagg agccaaagtc tggaaaaaat tgacggctga taatgttggg
    39301 aaaagcattg ccattgtact tgataattat gtgtattcgg cacctaatgt gatgggtgaa
    39361 attccaaacg gtcgttcatc cattaccgga cagtttgatg ttgctgaagc ccaggacctt
    39421 gcaactgttc ttaagtcagg taaactgcct gctcctgcgc gaatcgtcga ggaagctgtt
    39481 gtaggacctt cgcttggtat cgaggccatc aatgccggta tgttatcgtt tgtgatttcg
    39541 tttgtgctgg tgttgattta tatgatcttt ttctatagca aggccggtat tgctgccaat
    39601 attgctctgc tgaccaatat tgtacttatt tttggtattc tggcctctat gggcgctgtt
    39661 ctaaccttgc ctggtattgc cggtattgtg ctcaccattg gtatggcagt tgatgccaac
    39721 gttattattt atgagcgtgt caaagaggaa ttaagggcgg gaaaaggaac acggcttgcc
    39781 attgaggatg gtttcaaaaa cgctcttagt gccattattg atggtaacgt aaccacactc
    39841 ctttcaggtt ttattctttt gtggtttggt tcaggtcctg ttcagggttt tgctaccacc
    39901 ctcatcattg gtatttttac ctctctcttt actgctcttt tcattacccg ccttgttttt
    39961 atttggtggc tcgaccgaaa caaacctgtt gcattcttta ccaaaatcac tgaaaagctt
    40021 tttacagcaa caaagttcga ttttattaaa atgcgcaaat gggcttatgt tttctcagga
    40081 cttattttcg ttgctggtat tgcatccatt gctacccgcg gaatgagctg gggtgttgat
    40141 ttctcaggag gccgcaccta tgtggttcgc ttcgacaacg atgtgcaggt tgatgatatc
    40201 cgtggcgaat tgtttaaaaa tatgggcgaa acacctgatg taaagacctt tggatccaat
    40261 aatcaggtga aaatcactac caaatacatg gtggatgata attctgaaaa tgccgattct
    40321 ttggttgagg cgaaaatttt cgaatctctc aagggattct ttaataccag catgactttc
    40381 aaggatttca catccgatga tcccgataaa gtgctgggac gtttaagcag tcagaaagtc
    40441 ggaccaacca ttgccgacga catcaagcag tcgtcagttt acgctttgat tctggccttg
    40501 attgtgatat ttgcttatat tgccatccgg tttaaaaact ggcagtttgg tatgggtggt
    40561 gttgttgctt tgttccacga tgccatgctt gccatattca tgttttcttg gtttcatgga
    40621 atacttccct ttacccttga tgttgaccag acatttatcg cggcattgtt gaccattatc
    40681 ggatattcca tcaacgatac agtggttatt tttgaccgta tccgagaata cggggtttta
    40741 tatccaaccc gcgacaacag aaccaatatc aatgatgcag taaactcaac cattggtcgt
    40801 acaatcaata cagccggttc ggttatcatt gtgttgttag ccatttttat acttggtggt
    40861 gaaattatcc gcggattcag ctttgccatg cttgttggaa caatctctgg tacatattcc
    40921 acgttgttta tagcaacacc acttgcgtat gatatgctca agcgtaaaga agcaaagaaa
    40981 aaactgaaag cataattttg attgtttttg tggtttttac aatagccggg gtttcccggc
    41041 tatttttttg ataatcagca atttgcgaat ttagttatgc ttttatatgc gaaaaagtac
    41101 attttggtcg tttaaaaaac aaattagtaa ctaataattg tttaatttag caacatcaaa
    41161 aagtgttgct tattaaaact gcatcataag ggtatgaaaa acaggtttat attttatttg
    41221 ctcgttttgg ccgttgtagc tgtagctccg gtaaaacaat cgatggctca gaagaatttt
    41281 actgcagagg cagatgacaa gtttgctctc aacaagtatt acgatgccat agagttgtat
    41341 aaaaaggcct acaccaaagt gaaaaacaag gtcgaaaaaa acagaatcct tttccagatt
    41401 ggcacctgct attatttaat caatgacatg aaaaatgcag gtctgaacat gaaaagggtc
    41461 attaaggcca attatcctga agttgaagca tataatattt atgctcaaag tctcaaaatt
    41521 cagggtaatt acgaacaggc gctgattgcc ttcaatgatt tcaaggccaa ataccccgat
    41581 gataaacgtg ctgacgccgg aattgaatcg tgtaaactgg ccatttcatg gctcgatgct
    41641 ccaacacgtt atacggttga gcccgataaa aaactgaatt cacgcaatgc cgatttcagc
    41701 cctgcttatt ttgataaaaa atataaggcc attgttttca ctacttcccg cgaagaagta
    41761 aatggccgtg gcgaagataa ctggacagga cttcctttta ccgatttgtt tgttacatct
    41821 caggataaga aaggtaatta cagcacgcct gttcctcttg acgaagaagg cattgtgaat
    41881 tctgaagcca atgaaggttc atgtaccttc aactcaaaat tcaacaccat ttattttacc
    41941 cgttgccgta aagaaaagaa aaaaattctc ggttgtgaaa tttattcttc tgtgaaaaag
    42001 ggaagaagct gggctgagcc tgaattaatt ccgattgcgc ctgccgattc atttactgtt
    42061 ggacaccctt cgctgaccga tgatgaactg acgatgtatt ttgccagtaa catgcctgga
    42121 gggcagggtg gacgcgacat ttggatgata acacgtccca agaaaaacaa accttttgaa
    42181 aaagccgtaa atcttggcga aatagtcaat actccaggtg atgaaatgta tcctaccata
    42241 cgtgaaatcg gtggtaagaa gtatttgtac ttcgcatcaa acgggcatat tggtatgggc
    42301 ggacttgata ttttcagaag cgaatttgtt gacggaaaat ggacagcgcc tgtgaatatg
    42361 aaggctccgc ttaattcaag cagtgatgac tttgctatta ttttcaacga tgaccccaaa
    42421 atgctgaagg aagctgacgc caaggaaatg ggatggttta caaccaaccg caaaggtgga
    42481 cgtggcaatg acgatctctg gagattcaaa ctccctccga ttcttttcac actggcaggc
    42541 gtggtgcgtg atgacagcac caagcaaatt cttaaaggcg ctgaagtccg tattgaagga
    42601 tccaatggaa caatttatac tgactcaacc gaccagacag gatcttatta tttcgataat
    42661 atccagattg ttgaaaatac cacctataac atgatggttg cccttccgga ttattattcg
    42721 gaaaaaggca aggaaaccac agtgggcctc gaggtcagca aagatctggt tcatgattat
    42781 tatcttgttc ctattccgaa gaaacccatt gttttgcccg atattcttta tgacctggcg
    42841 agatgggaac tcaaacctca gtatgaagac agtctcgacg gtttggttac tattatgaaa
    42901 gaaaacccga cgctggtgat tgaacttggc tcgcatactg atatccgttc atctgacgat
    42961 tataatgata cattgtcgta caaacgtgca aaaagctgtg tcgattatat catcagtcag
    43021 ggtgttgaac ccgaccgact ggttccgaaa ggttatggcg aacgtgtccc gcgtgttctc
    43081 gagtccgata agcttgttgt ttataaaggc aaagagtacc ggttctccaa agggcaggtg
    43141 atgaccgaag atttcattaa tggtttggcc acagacggtg aaaaagaagc tgcacatcag
    43201 ttgaaccgcc gcacaacttt ccagattctc cgttcggatt atgtacccaa agccaacaca
    43261 aacacacctg ttaaccccga aattcagatt atcaacgaag gtggagaaga tgaaggcggc
    43321 gaagacgaag gtgaggatac aaataccgaa aatcctggcg gaggtgaagg tggtaactga
    43381 aaaaatcatc tgtaatctta ttggcctctg tttgattttt tgtaacagac attattaggt
    43441 tttttacgat caatgaatag cattcccttt tgtgagggaa tgctttttaa ttttgcaaat
    43501 atgaataacg acaaatatct gcatcggggt gtttcttcag gaaaagaaga agtgcatgcg
    43561 gctgccggaa aaattggaca tggaagttac gagcgcgctt tctgtcgtgc tgatgccgat
    43621 ccatctgatc cggaaaaact gattgtgctt cacgccgacg gtgccggcac aaaaagtgct
    43681 ctcgcttatg catactggaa acagaccggt gacctgagcg tatggcagga cattgctacc
    43741 gacgccatgg tgatgaatac cgatgatatg ctttgtgttg gcattgacaa tcatttcttt
    43801 gtttcttcga caatcggacg aaataaacaa ctggtttcgg gtgatgtgat tgcggcttta
    43861 attgaaggca acatccgttt ccgcgacaaa atgaaacgct atggaattga tgtagtgctt
    43921 tcgggtggcg aaacggccga cgttggtgat cttgtgcgaa ctataatcgt tgatgctaca
    43981 gcactcggat ttgtcaacag gaaagacttt atcagcaatg agaaaatttc agctggcgat
    44041 gtgattgttg ggctttcatc gtatgggcag tgtgagtggg aagaccgcta caacagtggt
    44101 attgggagca acggactcac atcggcgcga catgatattt tcgatttctc ggttggacaa
    44161 acttttcctg aaacctacga tccgattatc gaagcttttg cctatctggg gcgttataag
    44221 cttacgcagg catcgccggt agaaggactt gacatgggaa agtttgttct ttcaccaacc
    44281 agaacgtatt tgcctttaat gaaaaaagta tttgaaaaaa tgcgtagttc tgtgcatgga
    44341 ttggttcatt gcaccggcgg tggccaattc aaatgcatta aatttattga tcagcttaaa
    44401 gtgattaagg ataatttctt tgaagttcca ccggttttcg atttgattat gaaaagcgga
    44461 acagaagctg aagaaatgta tcgtacattc aatatggggc atcggcttga aatatatact
    44521 gatgagaaat cggcaaaaga aatgatgaaa atggccggag actgtggtat tgatgcccgg
    44581 gttgtcggtt attgcgaaac atcggataag gccgaagttg aacttaagac accctttgga
    44641 acatttaaaa aagaagttta aaaaatggat attctcggaa aactgaaaga agtacacgaa
    44701 aaatgggaag agattggccg catgctgatt gagccggatg ttattgccga tatgaagcgg
    44761 tatgtggcat tgaataaaga atataaagaa ttgcaatcgg tatcggaggc ctatcttgat
    44821 tattcaaatg tgttgtcaaa tatcgataat gcaaaaaaca tcctgcaaac tgaaaaagat
    44881 gatgagttta gagagatggc caaagcggaa cttgatgaat tactcatgaa aaaagagcag
    44941 atggaggaag aaattaaaat actgctgttg ccatcggatc cgcaggatag aaaaaactgc
    45001 atcattgaaa tccgcggtgg aaccggtgga gatgaggcca gtctttttgc aggtgatttg
    45061 ttccggatgt acacccgtta tctcgaaaac cgcaaatgga aatatgaaat tgttgatttt
    45121 acagaaggga cgatgggcgg ttataaggaa atcattatca atgtgaacgc cgatagtgcc
    45181 tatggtacgc tgaaatttga atcaggggtg catcgtgtcc aacgtgttcc cgaaactgag
    45241 gcgcaaggtc gtattcacac ttctgcggct acagtggcgg ttttacctga agccgacgaa
    45301 gttgatgttg atatcaaaga atccgacatc cgaaaagata ctttctgttc ttcgggcgct
    45361 ggcggtcagt cggttaatac tacttattct gccatcagac ttactcatat tccaaccggc
    45421 attgttgttc agtgccagga tgagcgcagt cagatcaaaa actacgataa ggcattgaaa
    45481 gtgcttcgca gccggatttt tgaaattgaa tatcagaaat atctcgacaa aattgccagt
    45541 cagcgtaaaa cccttgtttc gtcgggtgac cgctcagcaa aaatcagaac ctacaattat
    45601 ccgcaaagtc gtgttaccga tcatcggatt ggcatgacgg tgtataatct gccttcggta
    45661 atgaatggcg atattcagca atttatcgat gcattgcaga ttgccgaaaa tactgaaaga
    45721 ctcaaagaag gtgtaaatat ctaacccatt ttacctatga acagacagga acttaccaat
    45781 ctcgttttta aaaagaaatc ggtgctttgt gttgggctgg attcggaact ttccaaaatc
    45841 ccggcacata tcaaagaagg ccgtagcatt gaagaatctg tttttgaatt caataaggcc
    45901 attgttgacg ccaccattca atatgccgtt gcctacaaac ccaatcttgc attttacgaa
    45961 gcactgggat tgtccggtat gacagctttg cacaaaacgg ttgcctatat ccggtcgctc
    46021 agcgaaccgg ttttcattat tgctgatgcc aagcgtgctg atattggcaa cacgtcgcgt
    46081 ttgtatgctt ctgctttttt tggagatggc aaaacagctc ctgattttga cgcagtaaca
    46141 gttgctcctt atatgggaaa agattctgtg agtccttttc tttcttttga tggaaagtgg
    46201 gtgattttgc tggctttaac atctaacccg ggtgccgatg attttcagct tactgcaacg
    46261 cctgatggta tgcagctttt cgaaagagtg cttcagcagt cgaaacaatg gggtaacgct
    46321 gaaaatatga tgtatgttgt tggtgctacc cgtgctgaca tgctcaaaaa agtcagaaat
    46381 attgttccgg aaaatttttt attgattccc ggagttggcg ctcagggcgg agatttgaac
    46441 gaagtgttga aaaatgggtg gaataattcg ggtggactct tgataaatgc atccagatca
    46501 attatatttg catcatcggg gggtgatttt gcagaagctg ctgcaaaaga agcctgtcgt
    46561 atgcaaaacg aaatggcaca attcatcaat caactctgat tatgcggttt tatgcgttgt
    46621 ttttgtttct gataccactt tccttgtttt cacaaggtga tgactggttt gataacattg
    46681 aaaacatcac agataatgtt gaggagccta tgttgtatta tgagcgcttc aatgctttta
    46741 caatgggcga ctcggtccgt ttatgcaata agcgtccatg caacggaatt cagaaggatt
    46801 tctgggaaaa tggaattatt aaacataagg cctattatca gaatggacgc atcacaaacg
    46861 gatacgagaa ttatttcgac aatgagcaga tcgaacgcaa gtttaccatt atcaatgcca
    46921 gcagcgcaga acttgttgtt tattataaag atggaacact gcgctcaaaa gttgagtacc
    46981 gcagaaaaaa tgctgtaaaa tgggcagatt attaccccaa tggcaatctt gaattcattg
    47041 aggaatacga caacagcatg gagtattaca ttaaatataa tttttattat atgagtggtt
    47101 ctcctcagag tacccttgag ttggttgata aaaacgaacg tatttacacg tcgaaaacat
    47161 actataaaaa cgggcagttg aaagaagagg gcttcagaca gttgaatatg gctgcaggcg
    47221 attatgttcg aataggtgag tggaaatatt atgatgaaag cgggaaactc attgaaacac
    47281 agaatttttt caaaggagag gaaattgatg aagaggatga ataattttgt tgttgagaag
    47341 aaatgactat aataaaaaac cacttgattc aagtggtttt tttgtgatca gaattaacaa
    47401 accctttatg cgtcgatgcg cgcatatttt gcatttttct caatgaattc gcgtcgtggc
    47461 ggaacttcat ctcccatgag catagaaaaa atgcggtcgg cctcagctcc gttttcaatg
    47521 gtaacctgac gtagggtgcg ggtttcagga ctcatggtgg tgtgccagag ttgttcggca
    47581 ttcatttcac caagaccttt gtatcgttgc acatgaatgt ttttatcggt accgccttcg
    47641 cctttgaatt gctcggtcag agcatcacgt tgtgcatcgc tccatgcata ttgctcctta
    47701 ttaccttttt tcacgagata gagtggaggt gtggcaatat atacatatcc cagctcaatc
    47761 atttgcttca tataacggaa gaagaacgta agaataagtg ttgcaatgtg gctgccatca
    47821 acgtcggcat cggtcatgat gatgatctta tggtagcgga gctttgtcag gttgagttct
    47881 tttgaatctt cttctgtgcc aatggtaacg ccgaaagcgg taaaaatgtt tttgatttct
    47941 tcagagtcga aaattttgtg agccatggct ttttcgacgt tgagaatttt tccgcggagc
    48001 ggcagaatgg cctgaaactg gcgattgcgg ccttgtttgg ctgtgccacc tgcagaatca
    48061 ccctcaacaa gaaatatttc gcatgctgcc gggtcttttt cagaacaatc ggccagtttg
    48121 ccgggaaggc cgcttcctgt gagaatgttt ttgcgctgaa ccatttcacg cgctttgcgg
    48181 gctgcatggc gagcagtggc cgcaagaata accttattga caatggcttt ggcttctttc
    48241 gggtgttctt caagatagta attcagatat tccgaaatta ccgtatcaac aacccccatg
    48301 acttcgttgt tgccaagttt tgttttggtt tgcccttcaa actgaggttc ctgcacttta
    48361 accgaaataa tggcggtaag gccttcgcga aaatcatcgc cattaatatc aaatttcagt
    48421 ttcgaaagca ttccggattt ttcggcgtaa gcttttagtg tacgcgtaag tccgcgccgg
    48481 aagccagcca ggtgagttcc gccttcaatg gtgttgatat tatttacaaa ggtgtgtaca
    48541 ttttcggaga aggaggtgtt gtattgcatg gcaatttcca ttggaacgcc gccacgttca
    48601 ccttcgaagt aaatgggttc atcaatcaat ttctcacgtg ttccatcaag gtaattgata
    48661 aaatcacgaa gcccgtttgc tgaataatat tcgttgaaaa caggtttgcc atcttcttct
    48721 tcgcgctcat cggtgagagt gaggcggatt cctttgttga ggaaagccag ttctttcagc
    48781 cggctggcaa gaatatcata gacatattct tttattgtaa aaatggagtc atccggcaga
    48841 aattcaataa cagttcctgt tttgtcggtt gtgccaactt cttcaactcc ggttagtggt
    48901 tttccacatg aataattttg ctgatatatt tttccgtcgc ggtgaactgt ggctatcaga
    48961 cttgaagaaa gtgcattgac acaggatacg ccgaccccgt gcaaaccacc ggaaacctta
    49021 tacgatcctt tgtcaaattt cccaccggca tgaagaacgg tcatgactac ttcgagtgcc
    49081 gagcgctgct ctttttcatg aaagtcaacc ggaataccgc ggccattgtc ggtgaccatg
    49141 atggaattat ctttccggat gataacatca atgttgttgc agaatcccgc caaagcttca
    49201 tcaatggagt tgtcaactac ttcataaaca agatgatgca ggccacgctg gccaatgtcg
    49261 ccgatgtaca tggcaggcct ttttctcacg gcttcaagac cttcgagaac atgaatgctg
    49321 tcggctgaat aatcgtttcc gttattcttg atttttttgt cttttacttc tgtgttttca
    49381 gaatcaggag tcatactttt aattctttga aattcaataa attagcagga ttgctaatac
    49441 cgtacaaagg taagaaatta tactggaaat caaaagaaaa aaggcagcta tttcttataa
    49501 aaatttttac tcttgttagt ctcgcaaaat acatattaga taaggttttt aaaccattca
    49561 gtctaataag atttgctatt tgtttttaat tcgttctaaa accgaatgtt gttcttacgg
    49621 caattaactt ttcaggtagt cagtttttgc ggtttgataa ccttatgtaa tccgctggat
    49681 gcatatttag gtttgttttt taaggcatac atatatatat ttgttgcatt gaaacataaa
    49741 attacctcat atgaaaaaac tcaacttttt tgaattatcc ggtgtcatcc ggataatgct
    49801 gatgtccatc cttttttcat ggaccggctc aatggctaat aacgtgcgga tcaccaatgt
    49861 gaccaacacc aacccggggg cagccgaccc cattatatcg tttgatattg cctgggacaa
    49921 cagctggcgc gtatcaagcg gtccttctaa ttacgatgcg gtctggattt ttgttaaata
    49981 ccagaaagtt ccaacggggt ctcccaattg cgaatcttat caggaatggc atcatgcaaa
    50041 aatgaccaat gtccctggag acttcagtgt cggggctccg ctggatgttg tttttgtcgg
    50101 tgatagcatg gggatttatg ttcagcgttc agccgatgga attggcaatg tgagttctac
    50161 tactgtcacc atccgcctga accttgatgt tccggtttcg ccgtttgatc cggaaatgaa
    50221 tttcaaaatt tttggagttg aaatggtcta tgttcctcag ggtgactttg aacttgggga
    50281 tggaatcagc accaattcat ttaacagcat ctcaattaca aatacaacaa ctactctgcc
    50341 tgcggctact gttggtggca gtgttcattc tacaatccct tcggcattcc caaaaggata
    50401 ttcagctttt tatatgatga aatacgaggt aacccagcag caatatgtcg atttcctcaa
    50461 tacacttacc tttaatcaac agctgaaccg caccattctc acatcgactc agctggctac
    50521 tgatccgggt cttggtggtg tctgtgctat gatatccggt tgtctgaaca ggaatagtat
    50581 caaactgata caggagggaa tcaacgacag tcgtccggga gtttttgcct gtgattttca
    50641 acctgctccg ggcgatccct tcagcagcat caatgatgga caatcgattg ccaataatta
    50701 tatgagttat ggcgatttac tcgcttatct tgattgggct gcccttcgtc caatgactaa
    50761 ttttgaattt gaaaaaacaa gccggggacc cttgtctcgt gttggaaatg aatatatttg
    50821 gggttcgacc gatattaccc aagcgcagag cacgtcgctt aacagtgccg gtacagcatc
    50881 tgaaacatca actactgcag ggaacggtct ttcggcctat ggatctgcat caaccagcgg
    50941 accgcttcgt gttggtttta gtgcaactgc atctactgat cgggttacaa gtgcttcttc
    51001 ttattatggt gtgatgaatc ttggtggaaa tgtctgggaa atggttgccg gtggttcaaa
    51061 ttcagtcaat accggttaca cactcactta ttccagtctc ggtgatggtg agcttactgc
    51121 tgctggctta tacaatactt cgggttggca gacatataat tccattagct attcttcgtt
    51181 gggttgcggt tgttgttctt cgtatatcaa atggcatatg gaactcaggg gtggctcttt
    51241 tatgagtgcc agcacaacac tcagaacatc ggaccgcagt aaaaacagct ctgtaacttg
    51301 cggcggcggg ccggattttg gctttggtaa tgccggcgat cagcgtcaga gtgatgtagg
    51361 tggacgtgga gtacgttaat ctttttgcaa taaattcatt gctatgaaaa gaattcgttc
    51421 tccgattttc ttgcttctgt tttcattgat atcctctctg ggatttgccc aatatgcggg
    51481 cggtgatgga tacggaagca tttctctgtt gctcagccag acttcatgtt caacgccggt
    51541 ttatccttca atttttaccg gaggtgatga aacttgtgct gccgcttttc agttgcagca
    51601 agcttcgtgt gctgctgctg cactgccttc cgtttttgcc ggaggtgatg aaaattcggc
    51661 ttcgtcaatg ctcttttcgc aggtctcttg cgcaatccct gtatatcctt ccattttcaa
    51721 cggaggtatt gagtcttcag aaagttcgct caatgtaacg caggtttcat gcgctgctcc
    51781 tgtttatcct tccatttttt ccggcggttc cgacgatggt ttttcgggaa ctcctactat
    51841 tcaggtagca tgtattacgc ctcccgatgc tggaatttat gcgggcggaa acagcaacgt
    51901 cacaacgggt gtcactttcc tcaccgcctg cgctccttac gctgaattcg aagcagacac
    51961 acaatttgta tgtgaaggcg acacggtgtt tttcaccgac ctcagtcttg gcggcccaac
    52021 tgtctggagc tggtctttcg aaggtggaac tccgcttgga tcttcattgc aaaatccgtt
    52081 tgtcatctac aacacagccg gcacgtatca ggtgcagctc cagatttccg ctccgaccgg
    52141 aaacagcact gaattgaaaa caggttatat agttgtcggg gcaagtcctg ctgcaacaat
    52201 tgctgcttcc ggtccaactt cgttttgcgg aggagattcc gttcagctga ctgtgaatcc
    52261 ggcccatcct tcctatgcat ggaataccgg tcagacgaca caatcagtct atgcttcagt
    52321 gagcggaaat tactacagtg tagtaactgc agccaatggc tgtactgcaa atactgacac
    52381 catttcggtc aatgttctga ccaatcctgt tccattggtt acaacaagtg gcccgaccac
    52441 tttgtgtaac gctgatacac tgacactgac ctcttctccg ggaacatctt atttatggct
    52501 gccgggaagc gaaactacac agacactcga agtgaccgct tcagggacat actatgtaag
    52561 tgtgtcctat ccaaacggct gttcaaatgt ttcggctcct gtaaatgcag ttttcggtac
    52621 tactccggca acaccggtca ttactccttc aggcccgctc acattctgca gcggcgacag
    52681 tgttgtgctg acttcaagtc cggcttcttc gtatttatgg aatcccggat caacaaccgc
    52741 acagacaatc actgtgaaaa attccggcac atactatgtc gaaaccggca atggaacagg
    52801 atgtacagca atgtcacttc ctgtaaccgt aacggttttg ccaaacacac ctgctcctgt
    52861 aattactgca tccgggccgc ttacattctg caccggcgat agtgtggttc tgacttcatc
    52921 tcccgcggct acgtacaact ggtcccctgg tgcacagaca acacaaagta ttaccattca
    52981 taccgcaggt acatactttg ttgaagctga taacggaaac aattgtccgt ccgtatcaac
    53041 accggtgaca gtcacaatta atgctgttcc tgctacacca atcatcggtg catcgggtcc
    53101 gctcacattc tgtgcaggtg gcaatgttgt gctgaccgca tcagcagcgg gttcctacca
    53161 ttggtatccg aacggcgaga ccacgcaaag catcacagtc acagtatcgg gcaactatta
    53221 tgttgaaacc ggaaacggta caggctgcac tgcgtcttca actccggtga gtgtgaccgt
    53281 taatgcagtt ccttcaactc cggttgtaag tgcgtcgggc ccgctcacct tctgcaatgg
    53341 cagcagtgtg actttgactt cgacggcagc aacttcatac tattggtatc cgaatggtga
    53401 gacaacgcaa agcatcaccg tttctgcttc gggcaactat tatgttgaaa ccggcaatgg
    53461 aaccggctgt acagcatctt caacaccggt cgcagtaaca gtgaatgcag tcccggctat
    53521 accgaccatc ggtgcatcgg gtccgctcac attctgtgca ggtggcagtg ttgtgctgac
    53581 cgcatcagca gcgggttcct actattggta tccgaacggc gagaccacgc aaagcatcac
    53641 agttacggca tcgggcaact attatgttga gaccggaaac ggtacaggct gcactgcgtc
    53701 ttcaactccg gtgagtgtga ccgttaatgc agttccttca actccggttg taagtgcgtc
    53761 gggcccgctc accttctgca atggcagcag tgtgactttg acttcgacgg cnnnnnnnnn
    53821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgaccgtt aatgcagttc cttcaactcc
    53941 ggttgtaagt gcgtcgggcc cgctcacctt ctgcaatgga gacagtgtgg tactgacagc
    54001 atcagcagct gcttcctatc aatggtatcc cggaggacag actacacaaa gcattacagt
    54061 gtttgcaccg ggcaactatt atgttgaaac cggcaatgga agctgttctg cgcaatcagc
    54121 caccacgtcc gtagcggttc ttccgcgacc ggcagttcct gttgtttcag catcaggacc
    54181 gcttgtgttc tgtgatggtg acagcgttgt acttacaagt tcactggcag atgcctatga
    54241 atggaaccca tccggtggta catcgcaatc actgactgtg acagcgtccg ggacttactt
    54301 tgttgaagtc accaatgcat ccggctgttc gaattcatcg gtgccggtta cagtcactgt
    54361 caatccgaat ccggctgtca atcttaccgg accttccgtt gcatgtctga acactacgga
    54421 aaacttcact acgaccaatc ttggcggagt gagttatttc tggactaccg gcaatggtac
    54481 aatcgtatcc ggcagtggaa caaacgctgt gagtgtgttg ttccccgaca cgggtcaggc
    54541 gtcggttgaa gtgatcgtgt ctgatctggc aactacgtgc ttcagtcagg cggaaataat
    54601 tctgctggta ttgaatgcgc ctgaggccta tgcaggtgcc gatgtttcgg tatgtcaggg
    54661 aagttctgta caattgcagg catttggtgg aaccacctat tcatggactc cgccagccgg
    54721 attgtctgat ccggacattg caaatccgct tgcgacgccg gtttcgacca ccacctacat
    54781 tgttgaagtt gcgaacggaa gctgcacaga cgttgacaca gtattggtta ccgtgtatcc
    54841 aattcccgca gtggatgccg gctccgacgt gtacatcagt gaggactcct gcgttgtact
    54901 tgccggaagt ggcattggat ctgccctgtg gactcctgcc tatgggcttt cagaaacaaa
    54961 tacactaacg ccaactgcct gccccgacac aaccgtgacc tattatctga cggtaaccgg
    55021 cgctggtggc tgctttgcta ccgatagtgt aacggtatat gtttcggctt ccggcgatga
    55081 gcttgtattc ccgaatacat tcactccaaa tgccgacggt accaacgata tctggtatat
    55141 ttccggtctt gagcaatatc ccagtcatcg tctgactgtc tttaaccgtt ggggcaatca
    55201 ggtcttcgac gcagagccgt acgagaataa ctgggatgga accagtctgg gccgcgagtt
    55261 gcccgatgga acctattact atgtgttcga caagggcaat ggcgaagatc tgctcaaggg
    55321 atttttaatg ataataagat aaggaggcca ctatgaaaaa gatatggatt ttgatttttg
    55381 ttatcagtgt tcagactgct tttgcgcagc agcttccttt gctgagccag tactcgttca
    55441 acaagtatgt ttttaatccg gcagctgccg ggaacgagga tcgctttgaa attctgaccg
    55501 gctaccgcag ccagtggact ggatttaacg gagcaccgca aacagcttcc tttacagcgc
    55561 acggacctct ggggggcggc agctgcattg gcggcgatgc ttacagcgat gaactgggcg
    55621 ccacttcgga actcggtttt catcttacct atgcgtatca ggttcaggtg agcaacgatg
    55681 tgagatgggc atttggcctg caggcaggcg ctgtccagta ttccgttgat ggaacgttgc
    55741 ttacgaccta tgaacccaac gatcaggtgg taccgctgaa tatgaccagc gcattgattg
    55801 ctgatgccac tttcggaacg tatctgttca gtgagaaatt ctttgctgga gcgtcaatac
    55861 agcatctggc ggggagttct attttgttca gtaacgattt tccgaaagat caggttggat
    55921 tgctcgaccg gcatctggtt ttgcttggtg gaataaagct ggatgcaggt gataaactgg
    55981 cagtggagcc atctttcatg ttgcgtgccg taacgggtgc accgctgcag atcgatttct
    56041 cctcgcgtct gatttatgac aacaagtact ggttcggtct cacctatcgt acccgtgctg
    56101 catgggttgc tatgatcggg cttgatatct cggatcattt tgttctggga tatgcgtttg
    56161 attatgccac tactgagctg cagtcttttt ccgacggaac gcatgaaata attcttggct
    56221 ggaaattcaa taaaaagcaa cgttccaata tgttcatttg atttttcggt tgtagtattg
    56281 tttgaacggg tgctgtaatt ggcacccgtt tttttatgtt gtgaaagcac aaaatcgatg
    56341 atttcatatc cgccagtcat gtgctgggcc tgacgaagca tggcggatcg catataaaca
    56401 agcattatca gaagacgaag ctcattatat tcgactcctc cggagtcgtg gttttgtttg
    56461 atttgatcta cagatatatg actcctccgg agtcttccaa atccatggac ttttggtata
    56521 agagcccact taaagtacat tttaaccagc attgtttcac tataatatgc acaaaaatat
    56581 ttctatcgca aaacgctaca tagggtctgc tgaaaaacac gtaacactca caaaaacaga
    56641 aatatattca gcaa
//
LOCUS       DAOPKE010000009        52511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_651_length_52511_cov_11.175881, whole genome shotgun sequence.
ACCESSION   DAOPKE010000009 DAOPKE010000000
VERSION     DAOPKE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_651_length_52511_cov_11.175881"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKY63_02335"
     CDS             <1..602
                     /locus_tag="PKY63_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99482.1"
                     /translation="NPYTVTPASTTTYSVTGTDGSGCSAVASVAVTVNPLPTVSVVFP
                     AEVATVCKDSLVALSGGSPAGGVWSGTGVTGSDFLANTTGAFTITYTYTDANTCSNSA
                     TGTITVENCTSISEYSSNEMVVYPNPASDVITISFGKVDASQVEITLFDLSGRLVSNE
                     VLNLNGSSVEVSVNQLPAGVYTLKVKAGTDVFFTKITRL"
     gene            complement(1312..2601)
                     /locus_tag="PKY63_02340"
     CDS             complement(1312..2601)
                     /locus_tag="PKY63_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HPE99483.1"
                     /translation="MRKLFFFSLFLIVVSFLQVKAQELKPLLEKLQGVEVLEKAAVDG
                     YSESYILKVKMPVDHNDASKGSFTQRVFITHRSFDAPTVLVTEGYSAEHAKYPWYIEE
                     LTSMMNANQIVIEHRFFGKSIPDNCPWEYLNVRQAAADHHQIISMLKPFYKGKWVTTG
                     ASKGGSTCVYHRALYPNDVEATVSYVAPFTIADEDPRCITFLKDVSEDSIRAKIHAFQ
                     RAVLKNRESMKKYIAIDMKAAEDTLIMSIDSTLDYMVVEYPFAFFQYCGNPASIPSPD
                     VKPTTLYKHLKKIIAPTTYGIHDQNGTGAFFVQAYNEVGYYGYDTTGLGDLLSIKSGY
                     ISNRILVPKDVPVQYNPEMLEMVRNYIKTDAKNIIFIYGEDDPWSATAAVCGDNQNVV
                     FAMDKDNCHNVRIADMPDDLHMKIISMLEEWLAVDIK"
     gene            2696..2902
                     /gene="thiS"
                     /locus_tag="PKY63_02345"
     CDS             2696..2902
                     /gene="thiS"
                     /locus_tag="PKY63_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPE99484.1"
                     /translation="MKIILNNNIEILDENKVRTIRDLLELKNFSFKLLVVKINGRLIP
                     KTDYDVATIHDGDDVMILHLMSGG"
     gene            complement(3308..4228)
                     /locus_tag="PKY63_02350"
     CDS             complement(3308..4228)
                     /locus_tag="PKY63_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPE99485.1"
                     /translation="MKALFILFSFICAMAFNETFAQQFNPEKTRIKTKGLISPPINTY
                     LLCVVDIDYDGDMDFLGASDFGQVFLLNSGTSQLPSFESPKKRPFGLKGMMNATYADI
                     DNDGDLDAFGMKFSAVGTFKSLSLWSENTGTKSTPVFDKAVPFGFGMSENFFYKNFVD
                     IDGDGDLDLIGVYRTGQNIKESRVAMQENVGTAKTPEFSQVEFAPLGLIENSTAYIYF
                     SDIDGDGDFDAYYTAYTEETGKNVFIQWNEGNSTNAEFSDPHVGFTGMLISYGDNIFS
                     DINGDGIVENIIANDIGLFYQIMIVSKEEE"
     gene            complement(4302..5012)
                     /gene="ubiE"
                     /locus_tag="PKY63_02355"
     CDS             complement(4302..5012)
                     /gene="ubiE"
                     /locus_tag="PKY63_02355"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPE99486.1"
                     /translation="MSDPKAEEKKSNVVVDMFNDIAPTYDKINRRLSFSVDVIWRRKL
                     RKAVRKYNPQQILDMSAGTGDLTLELSKLHPEKLVAADPSEKMLEIAKLKLSKSCVMA
                     ELVKCNAEMAPFATNAFDLITCSFGVRNFASLEKGLKEMHRILAPGGHFVILEFGMPT
                     GFFWRHVYKFYFNHILPLRGRRLSRHNNAYTYLNQTVQQFPYGNDMEKILTDCGFEIV
                     RTKKLSGGIAWMYVVGKK"
     gene            complement(5012..6001)
                     /locus_tag="PKY63_02360"
     CDS             complement(5012..6001)
                     /locus_tag="PKY63_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPE99487.1"
                     /translation="MNFRENPFQKKKILIIGDVMLDAYIWGKVDRISPEAPVPVVSVE
                     KRESRMGGAANVALNISALDSTAILSAVLGDDDKAAQFENLLAENGMPRNGLLKSHDR
                     ITTTKFRIIGNNVQMLRVDEETDAPLPEELQQKFTKHVQDLIIANGFDAIIFEDYDKG
                     VIDAKLIESVVSEASKHNIPVLVDPKKRNFLHYKNVTVFKPNLKELRDALNKELPEEM
                     DELAAFCEEFRKQQNIRYLLLTLGERGMLLCHHENGKPGYIHMPTRVRKVADVSGAGD
                     TVIAVAALALAIGASPQEMAEWSNAAAGIVCEYVGVVPVDQERFLKSLSESRG"
     gene            6613..8067
                     /locus_tag="PKY63_02365"
     CDS             6613..8067
                     /locus_tag="PKY63_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPE99488.1"
                     /translation="MKKLHLLVLRAYAGPLVLTFCIAEFVLLMQFLWKYIDDLVGKGL
                     DFLIIGQLLFYASATFVPMALPLAILLASLMTMGNLGEHYELVAAKASGISFRKIMMP
                     LVLLSLFISGVAFFFSNQVLPVANLKMFSLLYDVKEQKPALNIQEGVFYRDIDGYVIK
                     VGEKDKDGQTIRRVLVYDHTKPGGNLSVTIAESGTMVTTDDKRTLIFTLYNGTNYTEP
                     RNNRESLTRRPMQRVHFSEEQLRFDLSSFSMSRTSEELFKEHYQMMNLEQLSTSIDTL
                     VTERGSRQDEFAHQLMRSSGYFYNFIVLQKRKPLAGNDSLADDVSRIRFDMSPTQYRT
                     AVGEAIGMARANAMMVDNYNKEMEAKQKILIKHEVEIQRKFTLSIACLLLFFIGAPLG
                     AIIRKGGLGMPLVVSVIVFILYYIISITGEKSVKEGALSPEFGMWISSMILLPFGIWL
                     TLKTTTDSPLMETDSWVKLAEKFNKFFRIKKKSE"
     gene            8064..9251
                     /locus_tag="PKY63_02370"
     CDS             8064..9251
                     /locus_tag="PKY63_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPE99489.1"
                     /translation="MRILQVCHKSPFPPSEGGPMAMKAIRDGLLAAGHHVHTFTLSTP
                     KFTVSDAMATKISYFSHSFIDTSVYIWPAFRDLCLNRSHHVSRFYNDKTAASLLQILQ
                     KSSFDAVLIESIFMMPYFKMIRKFFSGPIILRSHNVEHLIWERIAANETNILKKKYLQ
                     ILSKQLRRFEINCFLQADAIAAISSNDADSVRRLAPDADVVPIPFAIGLQNGPIPQKS
                     FCARFGHIGSMDWAPNLEGIDWFLNFVWPLVIKKCPQASFFLAGRNMPSRFVSNQSLN
                     LFVEGEVESSSQFMLSLDALIVPLFSGSGVRIKIAEALSLGVPVITTPVGSEGLLVKN
                     GRDIIISDNALQMTTDICRVIDNPDLLQNISENGLNTIRKHHSSETITQNLIALIERK
                     MKS"
     gene            9248..10414
                     /locus_tag="PKY63_02375"
     CDS             9248..10414
                     /locus_tag="PKY63_02375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99490.1"
                     /translation="MNRPKLFVILPRIPWPLEKGDKLRSYYFVKGLSEYYDITLFALY
                     DERYSNDVLEKIREISAEYHFFRIHIFSVLFHAILAIFTGKPFQVAYFTNYRLRRAVR
                     KAAQQQQPRAVFCQLVRTAQYARGLDCVKILDYQDTLSLGMKRRAASATGMQKWFFRS
                     EGKRLMRYEALVSDWFNHMLIITEQDRMELPVSDSRVHVIPNGVDTDYYSPVACDEIH
                     EMLFVGNMSYPPNINAMEYFVDCVLPGVLEHFPSAEIVIAGARPHSRVKILASDHVIV
                     TGWVDDPRDYYQRSAIFVAPMQIGTGLQNKLLEAMAMQRPCITTDLANNALGARHGDE
                     ILVAESLDEWIEMIVALLKNKDERNRIAINGRNFVIDKYSWPAQIKKLHQIIES"
     gene            10411..>11135
                     /locus_tag="PKY63_02380"
     CDS             10411..>11135
                     /locus_tag="PKY63_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPE99491.1"
                     /translation="MKDKSLISTVIIAFAIIVTGYLLGTNYKNRSQKPHTVEVTGLAE
                     KNFTSDLIVWNGSFSKFNKDMKLASNELKKDVEKVRAFLAERNIPENEILFSAVQINR
                     EYETTYDEDGNRKSEFVGYQLTQNVKIESKNIKLVEKVSRDVSALIDEGVEFYSDAPM
                     FYYTKLSDLKLQLIEEATKDGRARADKIAENSGGSIKRLMQGNMGVFQITARNSNEDY
                     SWGGAFNTSSLEKTASITVHLVF"
     assembly_gap    11136..11235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11236..13203
                     /locus_tag="PKY63_02385"
     CDS             <11236..13203
                     /locus_tag="PKY63_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPE99492.1"
                     /translation="MSSFQLPVEYVKEIPALLGEKDVFKALQLLPGVQKGNEGSSGLY
                     VRGGGPDQNLIILDDATVYNANHLFGFFSLFNGDALKSIELIKGGFPARYGGRLSSVV
                     DMNMKDGNREKLSGEGGIGIISSRLTLEGPVIKNKSSFLVSGRRTYIDALMRPFMNKD
                     NGTFGYYFYDLNAKINYEFDKKNRLYLSGYFGRDKFNFAESMDFTNSTSRLKAGLFWE
                     NATATLRWNHVFSNKVFSNASLIFSNYGLNIYEEEKYDDSRFSLNYSSGIRDLGLKYD
                     FTFYPNTYHTLRYGLQSTWHRFKPSAVVLEMTSTDEFVSEINSIHTFESSLYIEDEWK
                     WNRWRGNIGLRAFDFISDSCHYFSPEPRFAAGFMLTELSSIKASWTMMNQSVHLLSST
                     GMGLPTDLWVPSTKNIKPQKAWQAALGYARDLPKGLAMTIEAYYKKSDDVIGYREGAS
                     FLMITDPTDANQFSWEDNITVGQGWSYGGEFLLQKKTGKWTGWVGYTLSWTQLQFDEL
                     NSGQKFYAKYDRRHDISIVNMYKINDVFTLSCVWVFSSGNALNLPVETYDAVVDQNSQ
                     ADYMYNFYTYYYGPKNTFRLMPYHRLDIGLQHHQKREKSVHTWEIGLYNAYNRHNPYF
                     YFVDSGYNGENQLKQVSLFPMIPSFSYSIKF"
     gene            13206..14048
                     /locus_tag="PKY63_02390"
     CDS             13206..14048
                     /locus_tag="PKY63_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPE99493.1"
                     /translation="MKRKNLIFVLLPFLMISCEDMVSTVDVPQSGSHYVLHAYLSPED
                     SLVTVVAGKSKPVFGDQTTDTSWVSQSHVLVNGFELSRVPGPDYRYTAPAASFPVIPG
                     EFYTVELKVGTETVCSGSCTVPAWRNESMAFDGVDSVAYQSYEGTIYYDYYILYSFTD
                     LASEENFYRIAAEVTYVDPYSGDTSVFTTYPESSQFIKDVLFNGDQYSERIFLGGTEA
                     VSGLAGLRLMLYTSDRDYYFYHRAIVSQDGDDPFSEPVTVPSNVDNGLGCIAGVRKYT
                     LIVF"
     gene            14219..15574
                     /locus_tag="PKY63_02395"
     CDS             14219..15574
                     /locus_tag="PKY63_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99494.1"
                     /translation="MKKIVFLLLAVLILSSLPDFSFAQNPNKIAEREWKLLFEESIEW
                     YNFGFFENALRGFKRLLVKDRNNCNINFYIAMSYYYLRRPADVIIPYMEKASKKVDPY
                     YGYTYKETSAPVFSLLYLGQLYMNVYEFDKADTAFMRFGMYLTDKNRDAAYLMELSLW
                     MNYVNNARTQYANQLKNVSVENIKLINSGYNEGQPFLAPDGKTLFLTSDRKGSTGGQW
                     LNNVYKSDVYMVAIKNGRMYKPKKMGQKMNTSAADMCNSMGEGGKYLLISREDKNNKD
                     YNIYSSEISGKRFKEPQKLNPNINSKANEVHAFVSLNGNVMYFSSDRPGGYGGLDIYM
                     SEKMPNGEWGPAYNLGPTVNTWFDEDYPFVLDDGVTLFFSSNCTKSMGGFDIFVSTLS
                     EEGMWSDPENIGYPINTTSDDTGFMMTRDGLTGYYATAREAQSNGHIDNIDIYCVHFG
                     K"
     gene            15578..16183
                     /locus_tag="PKY63_02400"
     CDS             15578..16183
                     /locus_tag="PKY63_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99495.1"
                     /translation="MLRKVLTAFLLIASMCSPVLSQTGYALKEKDAYASCVDFIIVDD
                     SGKTVDVPVAVSRALQCPSLLSLHGDTLCYRSGNSIRMYHISSGADYQLFDVFDDVDG
                     VSGPVWSPSHRRIGFVIINQQRSHGYKDICRIIILDLDADFKVKFMHRYDRPVNFSCG
                     SICSSDAGFDFRFADENNFEYIRNINIEDSPGEKGYIFIGN"
     assembly_gap    16312..16411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16476..17609
                     /locus_tag="PKY63_02405"
     CDS             16476..17609
                     /locus_tag="PKY63_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99496.1"
                     /translation="MNSEFNPFPVSGYYGADYFCDRDSETNLIRRNIRNNINTTLFSV
                     RRLGKTGLIHHIIESYSKDRQVVCLYIDIFSSTCLMDFTNMLATVIFNRFPENNTIGK
                     KVMKAIASLRPVISYDTLSGDPEVRFEYMGASQYEKTLQQLFSFLDGQGKKIVFAIDE
                     FQQILEYPEKNMEAVLRTHIQRLKNTFFIFSGSNQKMMHEIFNNQKRPFYASCSNVNL
                     GFIDENIYADFIRQKFKQHKRSISDECIWFILEFTHRHTFYTQYFCNHLFASGIKRIV
                     KNDVYAAAIEILKLNESTCFQYRSLLTKAQWKLLTALAKEERLQKAHSVSFLKKYLLG
                     TSAHVTRGMAALIEKDLVYHNTSVEQPYYTIYDKFLMRWLQRL"
     gene            17712..18884
                     /locus_tag="PKY63_02410"
     CDS             17712..18884
                     /locus_tag="PKY63_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPE99497.1"
                     /translation="MISFEEAYDIVSSQPVIAGTETIRFDMASGRILAEDVCSDMEMP
                     PFDKSAVDGFACRIDDISQPMKIIETVPAGNMPSMQVVEGTCIRIMTGAPVPDGADMV
                     LMVEHCDVADEVVLYKGTSRNSNIAYRGEDMKTGALLIGASTLVSPQHIAVMAAVGKT
                     EIKVFSQIRAGVMSTGSELVEPDFKPSLAQIRNSNSWQLMAQITSHGGIPKYYGIIPD
                     DFDQTLLALKKAIEDNDIVFLSGGVSMGEFDFIPAALREAGFEILFDSVAVQPGKPTT
                     LARRDNKYIVGLPGNPVSSFVQFELLGSVLMNRIGGGIKDTVVVRAKTNIAFNRKKAE
                     RKSFYPVCFNANGYVASLEYHGSAHINSLLNAVGVVAMDIGVKEIPAETEVDVRLF"
     gene            18868..19668
                     /locus_tag="PKY63_02415"
     CDS             18868..19668
                     /locus_tag="PKY63_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE99498.1"
                     /translation="MFDCFNRHITYLRVSVTDRCNLRCTYCMPAEGVVLLSHNDILSF
                     NEIADVVRYGAEHGIKKVRITGGEPLVRKGVVQLVEMLSAIDGIEEMTMTSNGILLPV
                     FARDLKNAGLHRINISLDTLNPEKYASITRGGNLNDVIAGIDAAIEADLTPVKLNCVI
                     RNSPSEPDAQMVKHFADSRGIEVRFIHLMNLNTGEFSKVIGGDGGNCAMCNRLRLTAN
                     GNLIPCLFSNEEFNIRKLGIAKAYELALQNKPKSGTTSNTHGFYNTGG"
     gene            19941..20678
                     /locus_tag="PKY63_02420"
     CDS             19941..20678
                     /locus_tag="PKY63_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99499.1"
                     /translation="MNKTKYLIGIDDTDNLTSRGTGFIVRELARQLREAGIAYPHCVS
                     RHQLFFHPDIPYTSHNSSACLELDSEKPDELKTYCRTYLLNVAAEGSDAGLCIVAFDE
                     VSEEQIQWGLRAKKEILTKDAAQDLAARNHVFLEGLTGTRDGIIGSLAACGLRKFGCD
                     GRILMLAADFRSFQGSATAAQICTNFHIRNIFDIFDAPIPPDDLITLTEGWKPVLRNG
                     IYTLIVEKDGEHSWKSINKDYLRSISS"
     gene            20620..21186
                     /locus_tag="PKY63_02425"
     CDS             20620..21186
                     /locus_tag="PKY63_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99500.1"
                     /translation="MANTLGNQSIKTISGQSRRDIALAIQLLILFAAGFGAVVLHARF
                     RNPIGIPGHHGLEFMALAVTTALFFRFRLRGVVFSLGAGAALFLPFLGFTDPFAEFVF
                     MLPAIILCLLADAIKVSGKAATFVLMALGGLAYMSIPLARLAIHAVTGFPYKSLLVSP
                     FYVVFMHFVFGAAGAFLGYLIYRGLKRS"
     gene            21205..22095
                     /locus_tag="PKY63_02430"
     CDS             21205..22095
                     /locus_tag="PKY63_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99501.1"
                     /translation="MKKNTRPFIFLIFLAGLMFSMMMSCTPDENPGLPPVLKVTFADS
                     TSVSDTSIEAGQSIDIIVEGLGNENNITYFSLIRNGVHVLDSGLNAPGFVSRRTIVRG
                     TDSIENYTVLIRDRNFNETTFDFSIGLKPTLVYGGIRHWAHVVLGAQSNSVTGSFFDL
                     FSGQIFSLAQAFVNQDSVQLVYFYDAVDLNTIASPNANIDTSYFGGSSGLANWTVKNE
                     VRYIQIFNTEQEFSDCQNDSLIIANIFPYATGKRKAKVLQPGHMYEFSFAGLYGIFYV
                     HNVIGTDAGTIDISIKMQQP"
     gene            22092..24374
                     /locus_tag="PKY63_02435"
     CDS             22092..24374
                     /locus_tag="PKY63_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99502.1"
                     /translation="MRQFVFFIFGLLMSLQLFSQQTFTAIVRDAGTGKAIEGVTITVD
                     SIRLGAISGNKGEFKLRLPGKGPWVLRLRHIAYENTAWNVRKISDSLEIHEIIYLRQA
                     PHQINPVVITSDRVEKSTEEVPARMNVINSTSIESSPSTNIDEVLRSVPGMYVNRSWG
                     MYSRNSSVTMRGMGSASRVLVMLDGVPLNKSGGGGVNWNLLPNGAFEKIEVEKGPGSS
                     MYGNNAMTGTINLITGSPSEKERAEADFFVSQFNTQGIGFRFMTPSKARKPYVWLNIS
                     SMQGDGYFLDSDETRNEYSSKCFLNQYNAYLKAGYLISDSARFDVSVLGSGFKSGLGK
                     SVFEVNGNYDDFRTWMGSARYKVVRKKNTWNALVFANYEDYFNQSESINDYGEYKLSE
                     NPTEKLDAGVWTTMEHRFSKDLQMIGGIDCKYSVEDGENIYLTATDEIYFYGVHNFAA
                     AFVQVSKKLWSRSLLKAGFRYDFGQYNQGEILIENATAISDFLLPYAGQHPSSTWNSL
                     SPKLAYKFLMNEKFNVYASVSKGFMPPTIDDMTRSGKIRKGIKIANPFLEPEVLYNFE
                     TGCQWKPKENWTIEPAVYYSIADNMIYQVWTGEEVDLIGDGPKPLIQKRNVSQGTVAG
                     AEISINWTPRNWLNVMSAYAFNDSRISEYSSLPGDADLTGLFMAEVPKHLASIDIRAQ
                     YRIFTLTTEYRYTGETFADDENVDIIDDYHVVNMTLAARIKNYTIGISVNDLLDTQFI
                     DKKGYLSPGRFISARVNVKL"
     gene            24462..25358
                     /locus_tag="PKY63_02440"
     CDS             24462..25358
                     /locus_tag="PKY63_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99503.1"
                     /translation="MRTQIILFTLVLMALFSSCKKDDDSTTPPLLHLQLGGAYVSNNA
                     VLAINDTISFGVHAEGVSSNLTYFKVDVMSENGTSTIYDEGMNRKTFDATKIFFKGIP
                     DEETFVITVMDYNRNTTSASFTVFRDTLSGFGPIYHFTNLVLGYQNNSASGHFLDPNT
                     GIIYDDATVSGHESEVDIVVYFYLSSGSPSPTLVCPAQTDAQAQYPSMAGWPVQNATL
                     YDYHTSDYDLISAAQFDECTSDSLLIAAYNPTYVNQKCKFALTGKIVPFLTAEGKKGL
                     IKVVAADQAETGTMTIEIKIQQ"
     gene            25372..26229
                     /locus_tag="PKY63_02445"
     CDS             25372..26229
                     /locus_tag="PKY63_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99504.1"
                     /translation="MKKSLLLFVAMLGISMFSFAQLDTVIGFTFPVEVGLDSLDATIG
                     DTLNIGYDIRFESESAGTTDSVFLTNGATTYAATANSWEDGMNDKFFSIKFKTGYYKN
                     LVLYAKMRSGGTNAGPKYFKVQYKIGSSGTWTDVSTDTVTVANDWTTGVVDGWNLPSE
                     TWNASQSVYVRFLSITNTNVSGGTVDSLGVVKIDDIFVLGENNTGVGENSLMNTRVYP
                     NPANDVLNIENNDNVSVFFIISSDGRVVSQINNPATSIDISNLAAGHYMIRMISDDNS
                     MKTQSLIVE"
     gene            26290..28611
                     /locus_tag="PKY63_02450"
     CDS             26290..28611
                     /locus_tag="PKY63_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99505.1"
                     /translation="MKKLVLILIVLFPLLCAGQSVLYGYVFNASDKKAIAEASVRWIK
                     NKTGTTTKSDGTFSITYSSIQDDTLEISHIGYVILRKASSELFSENAKIFSFALTTLA
                     YNVSPVCITATREECDPMQIPQKVTLIDSASLHALPLQSIDDAFIYSSGVMIDRPMGI
                     FAAKSIVTMRGLSGSEQGRTLVLLDGIPINKSDGGTVNFNLVDPLLVDRMEIVKGPSS
                     ALYGGNAMGGVINIISAIPEEKLAGRVKLGYSTFNTLSGDMAVNSFLGKTADRGFFCS
                     GSAFWRESDGYISEPDYMRTEYTVPAFLKEKMFSLKGGYKFNAKSHFWISGMLFDDER
                     GGGEKVYEELGSFSEHDSYHIRSHYRTEAGKWKLTASGFYLRENYSKINEYVKADEYT
                     LYDVDSRRTDIGIIAHGSREIGKANRLTTGFEVRQGAVDAADVYHTSTDKIINAGIMD
                     MLALFAQDEFVFGKERWKLVTGLRYDYAGFHDGRFSIENPGMNNMYLLLYENDNMAEK
                     QWHSLSPKVALQFRMKENKRIYISYAHGFRAPILDDLCRSGKTRGGFQVANSDLVPET
                     VENFEIGYRHEINSKITVEPVIYYSIGNNFMYPVATGDTVDMGYAAPVIVTSNVSEVR
                     IAGAEIDFTYRINKNLNAFFNYAFAHSTVTEYTPAMAQQIDISGLYLSNVPTHLTNAG
                     IQYRYKNAGLSFNTRYVGSRWVNDTNTPDEKYGLPAEYDAYITADVKASYTYKKRWIV
                     SASVVNIADELYTDSKGQYCPGRMISFDAGIKF"
     gene            29325..30242
                     /locus_tag="PKY63_02455"
     CDS             29325..30242
                     /locus_tag="PKY63_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99506.1"
                     /translation="MNTMFGNKITAEIIARIFQRADEVARNEIVNGAVNDIKERDRVS
                     VLCREIRRNINKNPFIRSLSISAEAVDNKKGDALFVFRYKNEVKIGLVEAKILKINNA
                     NLDAYFDWIPNKHNYSHFTHQLKNLQKWVNEMAVWNMFIPNCPNGQYSPPLIAKGSSN
                     IWSSDLINHPKVSTPLVRWNFNDALNPPVQYMNLHTVIKSILNCKNGKVHIVSQGGRV
                     EVKALDDEIIDIPVPHFLGSIWGDLKQFSRKYYNINSYNYYRFDDLVEVVDDFKEKRV
                     LLKDPFIKREHFDEDEYIFFKQILNEIIK"
     gene            30436..32007
                     /locus_tag="PKY63_02460"
     CDS             30436..32007
                     /locus_tag="PKY63_02460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPE99507.1"
                     /translation="MAKQIKDKAIEVTLWDAANKLRGSVEPAEYKHVVLGLIFLKFAS
                     DKFEEHRARLIADGKEKYIEIADFYNMSNVFFLEETSRWSYLIKKSKQNDIALLIDTA
                     LHTIEKNNKALKGALPDNYFSRLGLDVTKLASLLDTINDIDTTRDPKQDLVGKVYEYF
                     LSKFALAEGKGKGEFYTPKSIVNLITEMIEPYKGIIYDPACGSGGMFVQSIKFIEAHH
                     GNKKEISIYGQEYTNTTYKLAKMNLAIRGISGNLGDKAADTFLDDQHKDLKADFIMAN
                     PPFNQKDWRAENELIDDPRWRGYDVPPKSNANYAWILNMVSKLSENGVAGFILANGAL
                     SGGGEEYKIRRKLIENNLVEAILVLPQDMFYTTNISVTLWIINKNKKEQNKTVGDETR
                     HYRDRATEILFMDLRQMGIPFEKKYTQFSEEDILKITGTYHQWQVSTPLNLTGYENIP
                     EFCYSASFDEVAKKDFSLVPSKYIEFVNRDENIDFDEKMKAIQGELADLLKAEASSKD
                     DLLTVFKELGYEIKF"
     gene            32132..33220
                     /locus_tag="PKY63_02465"
     CDS             32132..33220
                     /locus_tag="PKY63_02465"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPE99508.1"
                     /translation="MENYKIIRKNQFACSTMQVRRDKKMPVALLQNSDEAIISQAYPV
                     FEVKDEKELLPEYLMMWFSRSEFDREACFNAVGGVRGSLEWEDFENMQLPIPHPDKQR
                     ETVAEYNIIQNRIKLNEQLIQKLEETAQAVYREWFVEFEFPDENGKPYKSNGGEMVWC
                     EELGKEIPKGWKNVSLSSIIDIKDGTHDSPSPTENGFPLVTSTHLNPYDISLNETYNI
                     SERDYIQTNKRSKVEKFDILFSMIGTVGLVNYVLYDDINFAIKNVGLFKTSEKKEFAE
                     YILFYLKSDYMKLYIASNPNGSTQNYVTLGFLRDMPILLPLKKIILQFESISEKIIET
                     ILLLTKENQKLMELKELLLSRMAKLGKI"
     gene            33226..35106
                     /locus_tag="PKY63_02470"
     CDS             33226..35106
                     /locus_tag="PKY63_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99509.1"
                     /translation="MKINLKQAVRAFFQNPSLEMVFIEAVANSIDANATQISIDISMK
                     SLSHQESLEITITDNGEGFTDERFKKFGELLSVNENTHKGVGRLVYLAYFDEILVESQ
                     YGKTKRTFTYDASFDEDKSDMKTNPEVDEKFETKIVFKKCSLQRLSSYNVVLPDYLKR
                     RLLEEFYPKFYLKKEEKNRLDVSIGIHVSEISKNISIGNRKTIIKVTDIPEMKTANVD
                     ARQIQLFENMDLKYSIIKRNDFSSPFIITALCIDDRTYNIKDVISDENVPNGYECVFL
                     LSSTVFDGQVDASRQILQLNDTIKRGVVKLFRDKISEIFKDKIPEIAQKNEKTKESLS
                     KTFPHLVGYFEEEEIGVISRDKSIEIAQKKYLRDQKVILESDSLDDDRFDKTIEISSR
                     ALAEYIIYREKIIEKIRTITTEDPEAEIHNLIMPKRAIYRGDDVLSNVYSNNLWLLDN
                     KYMTYSVAMSERTMKEIAEEISDEIQSGTDNNKPDIAIIFSDNPQVENKKVDVVIIEF
                     KKRGIKLAKTEEVISQLRQRARKLMRYYPEKIQRIWFYGIVEFTNEFKLSLKDDEYTP
                     LFSKDKLYYKENKIHLSEDSDEYHLVGTYILSIDAFIEDAKAQNETFLKILKSGFLKS
                     KH"
     gene            35113..38343
                     /locus_tag="PKY63_02475"
     CDS             35113..38343
                     /locus_tag="PKY63_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HPE99510.1"
                     /translation="MNNNQPKLIKNQPIGEDKFEGQAQENIAKAVVKMLEENSCQMIG
                     IDGGWGTGKSNLVGIVEKELNKSSKGNFHVFTYDAWGHQGDLHRKAILQELIEFLCKT
                     KKVLKWDEWKEKLKTLLGTSVETRHKTTPKLSLGIIFSFLIFLSIPIFNSIANDKDTE
                     TATKIWIQLIPVGALILLYLGYLIKELLRWISGKSKLRSIFSIAASEMIYVYKGKELE
                     TTATEYKHESNPSVFDFRSFMEDISTAIGNKRLIFVVDNFDRLPINQVKELWASIHTL
                     FAEKNKCSNISVIVPFDRAHIKSAFRDEDTPATSDEANCYGNDFINKTFDVIFRVSVP
                     ILSDWEHYFKAKWEDAFGKISSENETEYNAVVQIYDLLTQKITPREIIAFINECVSTR
                     ITQREEIPFRYIALFIKGKDKILKNPIEEITTPTYLGAVDFLYNYDENLPKYISALVY
                     QVDSSKALDVAYKKVLKDALEGNDQKVVVKISASKSFYSILKNTINEVTNIENLILAL
                     ENIPQESFKSPSNSETIWTCIFKRAEIKTDGRPGLQNYQRALLTKISIAEKERYTRKL
                     LAGFTSSSEFNAVTYANNIETIDAILRPVNIDVYSYLSDKLTGLNDFVSLLNSQYQKP
                     AQYRIKCNPDEIDVTLEGKTIEQLKDISYVPKIIEEYSLVKYKAKLIQWIPTALLHIS
                     QLEILFDRIKEVQGSIPATIIPDAYIKSHFISTGEDSNIYPDLISMRIARQNEFPAPL
                     ANDFEVVLNSANENLIDRVTRCIHFYISIDKLLLGLGDFGQYPLYRGIVQRLIMSPSN
                     KKIENIEPLLSNFEMICSNGEISPEQLMNFLNSNYTTEFTPTNVPVNIPVYLITSISQ
                     NESTLSERCRNLVLEYLSTMNEEKWAVAFSNINSYEVHASLLLKINWNTVAHIAFKKT
                     LKKIADGGIAISNKERWDELVQSFVDQRTMESAFNDVTDTLCNSSKDFSNDMFVFLAP
                     WLFEHSTSLLSKKEVLRKIFPPTILTDERCLHILNSNREKMIQVLASAGAESYDFKDA
                     LIDHARGENPSFRQLAESLKIDIPAEPEESEEDTNNEADNNETIDK"
     gene            38384..38863
                     /locus_tag="PKY63_02480"
     CDS             38384..38863
                     /locus_tag="PKY63_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99511.1"
                     /translation="MHQIKQLYAITQQLREKYAKWNKQFTLDGKLVGDMGEVLAAEKY
                     GLELYDENYEIYDGWETATGRKVQIKASFKGYFQFPYGENKIPEYYLAVIIDEHGVLH
                     EVYNGPGQFVYDNYVVKNNLKPYKNSYYTVSKGKLSSLNDVVPKEDKIIEVTTAKEN"
     gene            38866..39975
                     /locus_tag="PKY63_02485"
     CDS             38866..39975
                     /locus_tag="PKY63_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPE99512.1"
                     /translation="MDFQQLKVFLQTHEIPVSEETIGFLEIIGKQHHENINSTIYAHF
                     INCGHDVVRNLFVESLCEIVSERSGKSLNLKLALARTEVNTGSGRIDILISDQGNQNA
                     IIIENKIYHFLGNELIDYWQHVKQPDDKKVGVLLTLYPHEIPENVSGKFINVTHIEWI
                     KMVKNRLKPEILPWKYAVYISDFISTIENLTGDYPMNESVKFYFQHAGQILAANQAMR
                     QTHDFINNQFEIIAKRIGWSTYGGSMDWRNFWDERNKLDTYLTIIVEDLLKGKLCFTL
                     ILELGERDIVYSDKLRNELKACLKTGMYWGKHEVNYIHLLCKNYEIELEDLEHFADFV
                     ERKIREDFAELTHKAIKCLYPETDISHWENHFIGA"
     gene            40295..41020
                     /locus_tag="PKY63_02490"
     CDS             40295..41020
                     /locus_tag="PKY63_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HPE99513.1"
                     /translation="MNVADYISGKIERLPKGYVFTYADFDTKVNQKEAVIKALNRMVQ
                     SGKIVKLSKGRYYKPETTPFGNLLPDQKQVVKDLLEENGKPTGYLTGYSIYNQLGLTT
                     QVSNTIQIGKNQVRPGFKRERYTIAFVKQKNTITKENIPLLQLLDAIRYIKKIPDAST
                     EASCKRFLSIIRKFSDKEISTLGRLALKYPPATRALLGALLDQLKKGAVTEPLFKTLN
                     PITKYKLAGAVKALSTAEKWNII"
     gene            41017..42000
                     /locus_tag="PKY63_02495"
     CDS             41017..42000
                     /locus_tag="PKY63_02495"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPE99514.1"
                     /translation="MRLHEDKILFKQAVRYASDQMQIPAVYIEKDYWVTYALSAIFKN
                     EAGKDVVFKGGTALSKCFKMIDRFSEDIDLVVLRRDGESDARMKTKLKNVSNAIEPVL
                     PEIEIDDITRKRGMNRKTAHRYSKEFDGNFGQVRDVIILETTWLGYYEPFTSSSIVSF
                     VGQMMLDNNQTDIAEKYALLPFDLLVLEPTRTLCEKIMSLVRFSYGKNPIEDLKKKIR
                     HTYDLHQLLKHKEFAEFFKSVAFEKMLLKVADDDVRSFKNNNEWLKFHPNDAMFFAEL
                     VQVWDKLKTVYSGDFKKLVYGSFPDEFEVYQTLKQIKERMSTIQWSINIQS"
     gene            42003..45242
                     /locus_tag="PKY63_02500"
     CDS             42003..45242
                     /locus_tag="PKY63_02500"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPE99515.1"
                     /translation="MKFTEASLEQSFVELLLSEGYAHVHGDMISKAPGEVLIEEDLMR
                     FLLSEYAKDGLTETEARSIVLQLQRLPASDLYNSNCTFMRMLSDGFILKREDRNQKDI
                     YIQLVNYAGLNRHRQPDAETLITIAAEPEATYPPDTNIYKFVNQLEIHGNEKRIPDGI
                     VYINGLPLVVFEFKSAIREEATIHDAYKQLTIRYRRDIPELFKYNAFCVISDGVNNKA
                     GSFFAPYEFYYAWRRVAGSAKEVDGIDGMYTLIQGMFHKDRLRDIIRNFVYMPDSSKK
                     EEKIVCRYPQYYAAKALFENIRKSHRPQGDGKGGTYFGATGCGKSFTMLYLARLLMKS
                     EYFESPTIVMITDRTDLDDQLSGQFTNAKKFIGDNTIISVESRAHLRELLQDRQSGGV
                     FLTTIHKFTEDTELLTDRTNVICISDEAHRSQVNLDQKIKVTDKGVKKTFGFARYLHD
                     SLPGATFVGFTGTPIDATLDVFGKVVDAYTMTESVKDEITVRIVYEGRAAKVALKNGE
                     LEKIEKYYEEAAAAGANEYQIEQSKEETANMYAILGDPKRLKAVAEDFVNHYCNRVSE
                     GATVRGKAMFVCSNREIAYELYKNIIEIKPEWNEVRAADPACAGTADREGAELTDKDR
                     RELKPVEQIKMVMTRGKDDPDELFELLGTKESRKELDRQFKNEKSNFKIAIVVDMWLT
                     GFDVPFLDTIYIDKPIQQHNLIQTISRVNRRYEGKNKGLVVDYIGIKKQMNLALAKYN
                     KGDEKNFEDITESLIVVRNHLDLLDKLFHTFDKSKYFKGTTIQQLNTLNLAAEFVQQT
                     KDFETRFMGFVKRLKAAYDICAGSDQLTQPERDYTHFYMAVRSIVFKLTKGNAPDTAQ
                     MNARVREMIKDALQSDGVEEIFRIGDKNENGQDLFDEDYLAKIDKIKLPNTKIKLLQQ
                     LLARVIGEIKKVNKVKGVDFTKKMQAIVERYNNRDVNDILRSEVYEEMAEALTNLIWD
                     VQKEFRAGDELGIDFEEKAFYDILKELCIKYDFIYPEDKMIELAKAVKELVDGQAKFP
                     DWNKRDDIKSALKVGLILLLDEFGYPPVERDEVYVEIFEQAENFKKNQN"
     gene            45423..45908
                     /locus_tag="PKY63_02505"
     CDS             45423..45908
                     /locus_tag="PKY63_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99516.1"
                     /translation="MDKIKFRYHLDEATKQLIVMTKTLCYNDFSDNYNYILTPNLRTV
                     DKDDKHLNEKEISVLNSWNKFEGKLLTATEVVDLFHHDNKVPVWIDMTVYEARPDLTV
                     IDLFCSRRLREEKELMHQGLHPFHVQVATPPDSLKVEIDGKFDVNWKKKRDDHNKKGG
                     F"
     gene            46071..46529
                     /locus_tag="PKY63_02510"
     CDS             46071..46529
                     /locus_tag="PKY63_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99517.1"
                     /translation="MRTFLFITCALIINLTSGQTAKDYFNEEVKDAQLATAQTIMTAL
                     ENGKIELAGMFFDDSIKNLRSELNYISSEISKVKAETKLSIVIVFDQASNIYRCRYSN
                     DSDELFQVDLFMSEGQANSKVKKLEIKNSDVLKMEAKERMEMKEPPLPPN"
     gene            46592..47038
                     /gene="moaC"
                     /locus_tag="PKY63_02515"
     CDS             46592..47038
                     /gene="moaC"
                     /locus_tag="PKY63_02515"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPE99518.1"
                     /translation="MKKLSHTDTKGKARMVDVGEKPIQSRTAIASGKIILQPATIKLI
                     KENGMKKGEVLSIAEFAGIMGAKKTSELIPLCHPLLLTDVQVSAKLIKNGVEISATVK
                     CQGQTGVEMEALTAVSVGLLTIYDMCKAVDKKMILLDIELLSKTKI"
     gene            47058..48194
                     /locus_tag="PKY63_02520"
     CDS             47058..48194
                     /locus_tag="PKY63_02520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPE99519.1"
                     /translation="MKIVKRIFLGLLILIVIANILILVSGNSYIYKTLIYQHVGIEDF
                     EIFENRIVEAGTPTEIPNGTYKTELPDSFRKFMVETQTTAFLVLQNDSIIYEEYWDGY
                     GPDTVSGSFSVAKTIVSIMVGIAIDEGKISSVKQKVSDFLPEFSEGLKTELTIEHLLT
                     MTAGFDWDEAYASLFSPTTKAYYGNNLRKMVDKLDMKVKPGTLHEYQSINQVVLAMIL
                     EKATGKTLSEYTSEKLWTPLGATHDALWSLDREDGLEKAYCCFNSNARDFSRIGSLYL
                     HKGELNGKRIVSEAYVLNSVNPCMIPDETGSPCDYYGYSWWLSTIDNHAFFYARGILG
                     QYIIVVPEYNLVIVRLGRIRPDHGDIQAADYIVSNILKTLKTNE"
     gene            48187..49119
                     /locus_tag="PKY63_02525"
     CDS             48187..49119
                     /locus_tag="PKY63_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPE99520.1"
                     /translation="MNRIEIKSVNVSDKKGIVKVPVEAIELCETGIIGDAHAGAWHRM
                     VSMLAFESVEKFEHKAKRKIAFGEFAENITTQGLLLYETHPLDRFEGNGIELEVTQIG
                     KECHGDSCAIFREVGNCVMPKEGIFVRVLKGGKMKPGDTMEYKPKVHKIHVITLSDRA
                     FAGEYVDLSGPAIESKLKSFFESEKRVCEIQRTVIADDSEALETLIVHSVNAGFDAIF
                     TTGGTGIGPRDITPETVWPLLDKDIPGIMENIRLKSAQKNPNALLSRSIAGVIGNTQI
                     YTLPGSVKACNEYVEEILKTYIHLLLMLCKVDAH"
     gene            complement(49137..49721)
                     /gene="thiF"
                     /locus_tag="PKY63_02530"
     CDS             complement(49137..49721)
                     /gene="thiF"
                     /locus_tag="PKY63_02530"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPE99521.1"
                     /translation="MTFEEIKKKVSQYTVGIAGCGGLGSNCAAMLVRSGVTKLIIADF
                     DVIQKSNLNRQFFFYDQIDKPKADTLKKNLLMIDPHAQIESHVIRLDREKIELLFAPC
                     DVIVEAFDLAENKQMLIETVLDIFPEKPLVCASGLGGLDDADQMRVVRNGNLIVVGDF
                     LESVSEKNPPMAPKVTIAAALQANEVLRLLLCEV"
     gene            49863..52022
                     /locus_tag="PKY63_02535"
     CDS             49863..52022
                     /locus_tag="PKY63_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE99522.1"
                     /translation="MAINIEDLKTNRKILASFDYQIAPVNRGYTDKTLYINVGSGEIK
                     SKDVPAQVKEKFIGGKGYGLRLLWDAVTPNTKWNDPENEINISGGPICGITQYSGAGK
                     SIVVTLSPQTHIPVDSNVGGFFGPFLKIAGFDALELQGKAEKDVVVYIDAAKGRVEIF
                     EDPGFEHDSHMLAEELTDLFANDEKEKKNIGIVSAGDAADHSYIGMLNFTFYDNKRKK
                     IRLKQAGRGGIGTVFRDKKIRAVVAKVPGVTGNLNNVVDLDTIMERGRRFNKEMRELD
                     DSQCEMRKKGTAHLTNIMNDYDLLPVHNFKFGVHSDAGKIHGDVWMKYFTQGVPDGCW
                     IGCNMSCAKGVDDFELKTGPYKGQKVIVDGPEYETTSSLGSIMGIFDPEWTIEANFYC
                     DTYGICTISWGTIMGFVMECYENGILNKERTGGLDLSFGSKDDAMELLHQVARGEGFG
                     PTAGKGIRYMKDLFIANGWGAPQFIEDIGMENKGLEYSQYISKESLAQQGGYAMTNKG
                     PQHDEAWLIFMDMVNNQIPTFEKKAEALFYFPMFRTWFGLQGLCKLPWNDVEPADNAQ
                     TAEPAKVPEHVDNYVAIYKAVTGNDFSKEEMIRQSERVYNFQRVFNIRMGKGLRKNDA
                     QPYRACGPVTKEEYESRVERYDKQMKELIGIDPEGKTTEEKMAITRKYREDRYEQLLD
                     AVYSRRGWTKNGVPTIEHMKELGMDLPEVIEVIKPLQ"
BASE COUNT        15571 a        10206 c        11508 g        15026 t
ORIGIN      
        1 caaacccata tacagtgact cctgcatcaa caacaaccta tagtgtaacc ggtacggatg
       61 gttccggctg ctcagctgta gcttcagttg cagtcactgt caatccgctg cccactgtat
      121 cagtggtctt cccggccgaa gtggcaactg tttgcaaaga cagtctggtt gcgctttccg
      181 gaggttcacc tgcaggtggt gtctggagcg gtaccggtgt taccggaagt gatttccttg
      241 ccaatacaac aggcgctttc actattactt atacttacac tgatgccaat acctgttcaa
      301 acagcgctac aggaaccatt acggttgaga actgcacctc aatcagcgag tacagcagca
      361 acgaaatggt tgtctacccg aaccctgcca gcgatgtcat taccatttca ttcggtaaag
      421 tggatgcatc acaggttgaa atcaccctgt ttgatctttc gggtcgtctc gtaagcaacg
      481 aggttctgaa tctgaacgga tcaagtgttg aggtttctgt gaaccagctc ccggccggtg
      541 tttacacatt gaaagtaaaa gccggaaccg atgtattctt cacaaagatt acaagactct
      601 gataatttta gaaattctga aaaaggctgc cttcgggtgg ccttttttaa taaatacgaa
      661 atcagaaata cgagatacga aattagaaac aagaaatacg agattggatg tgaaaatgtg
      721 gagacttcgt ccatgtgcgg cttcgacgat gtggaaatat atgattctgg aaaagatgtg
      781 gaaatgtgag atcaggcgga agttataatg cgttagtagt tgtgagtttt gagttataaa
      841 ttataagtta tgagcgagaa cttgtaacga ttgtaacatg tacgattgta acgactcagc
      901 tccttctcag agctgaaagc tgactgctga gggcggcttg gtcttgacct tgcacctcaa
      961 aggagcttca cgtagaacgt gacgtggtcg cacaccttgt ccctggggcc tttggcccaa
     1021 ggctgagata tttagcgcct tcagcgcttc cgatggtgct tcacgtaaaa cgtgacaagt
     1081 cgcagctttc tttgatacct tcggactcat tgtgtgttgt gcattgttcg ttttgccatg
     1141 gactgcaagc cgcatgccac ctgccaactt ctcagcaccg tctcagagcg ccgacgctta
     1201 ggtcggcacc tgctgctgaa agctgcttaa tattgtaacg atttccgctt tcagcgtgat
     1261 aaattccacg tctgtaacga tagtaacgag taacgcctgt aacgaaagtt tctatttaat
     1321 atccaccgcc agccattcct caagcattga aataattttc atatgcaaat catcaggcat
     1381 atctgctatg cgcacattgt ggcaattatc tttatccatg gcaaaaacaa cattctgatt
     1441 atcgccacaa acggctgctg tagcgctcca cggatcatcc tcgccataaa tgaaaataat
     1501 gttttttgca tctgtcttta tgtaattgcg caccatttca agcatttcgg gattatactg
     1561 taccggaaca tcttttggca ccagaatccg gttgctgatg tagcccgatt tgattgaaag
     1621 cagatcacca aggcctgttg tgtcatatcc atagtaacca acttcgttgt aggcctgtac
     1681 gaaaaacgct ccggtaccat tctggtcatg gattccgtag gttgtgggtg caatgatttt
     1741 tttcaaatgc ttatacagtg tagttggctt tacatccgga gatggaatgg acgctggatt
     1801 tccgcaatac tgaaaaaacg cgaatggata ttcaaccacc atgtaatcca gtgtgctgtc
     1861 aatgctcata atcagtgtgt cttcagcggc tttcatatca atggctatgt actttttcat
     1921 gctctctctg tttttcagaa ctgcccgttg aaaagcatga atttttgcgc gtatggaatc
     1981 ttcacttaca tccttcagaa acgtgatgca gcgcggatct tcatctgcaa ttgtaaatgg
     2041 cgccacatac gaaacagttg cttctacatc gttgggatac agtgctcggt gatacacaca
     2101 ggtgcttccg cctttgctgg cgccggttgt aacccatttg cccttgtaga aaggtttcag
     2161 catggaaatg atctgatgat gatcggccgc agcctgcctt acgttcagat attcccaggg
     2221 acaattgtca ggaatgcttt ttccgaaaaa acggtgctca atcacaatct ggttggcatt
     2281 catcatcgat gtcagttctt cgatgtacca tggatatttg gcatgttccg ccgaataacc
     2341 ctcggttaca agaacagtcg gcgcatcgaa actgcgatga gtaataaaaa cccgctgggt
     2401 gaaagaccct ttgctggcat cgttgtgatc aaccggcatc tttactttta gaatatagga
     2461 ttcgctgtat ccatcaacgg cagctttttc aagcacttca actccctgga gcttttcgag
     2521 aagtggtttc agttcctgtg cttttacctg aagaaaggaa acgacaatca gaaacagact
     2581 gaagaaaaaa agttttcgca ttttattttt ttgcaatgat acaaaatctg tgcgtgatgg
     2641 aattgcagaa tcgataaaaa tcccttatgt ttgtacatcc aatttctgaa tcacgatgaa
     2701 aatcattctc aataataaca ttgaaatact tgatgaaaac aaagtccgga caatcagaga
     2761 cttgttggaa ttgaagaatt tttctttcaa gcttctggtt gtaaaaatca atggccggct
     2821 tattcccaaa accgactacg atgttgccac cattcacgac ggagatgatg tgatgatttt
     2881 gcatctgatg tcgggtgggt gagacttgca gcgcaaagcg atgcaagttg aaagtttata
     2941 aagtttctaa agttgaaagg caacgtaaac gcatacggca tggagcatgc ggcttgcgga
     3001 tcttcgctct cgctctgaag ctaagctgag ccccgcccgc tgtcattcag agtgtccgcc
     3061 agctggcgga tgtatcgaag aacgacagcg ggtttgcaat atgtcgcact tcgatacgat
     3121 gaagactatt gaatcataat gctgaatact tcatcactca gtgtgacaat cgcttttgct
     3181 gcagcagtaa aaatcaacag aagaccttat tgagttcctc tgacggtacc gtcgggggat
     3241 ctctttatat acttcagact ccgcttctgt tgattttaaa ttggcggatc gttgaaagaa
     3301 ccagagatta ttcctcttct ttactaacta tcattatctg atagaacaat ccgatatcat
     3361 ttgcaataat attttccact atcccatctc cgttgatatc agagaaaata ttatcaccat
     3421 atgatatcaa catgccggta aatcctacat gaggatcaga aaactctgca ttcgttgaat
     3481 tgccctcatt ccattgaata aatacatttt ttccagtttc ttctgtataa gcagtataat
     3541 aagcatcaaa atcgccatcg ccatcaatgt ccgaaaaata gatgtaagca gttgaattct
     3601 ctatgaggcc gagtggtgca aattccactt gtgaaaattc aggtgtcttt gctgtcccaa
     3661 cattttcctg catggcaact cttgattcct taatattttg tccggttctg tacacgccga
     3721 ttaaatcaag atctccatca ccgtcaatat caacaaaatt cttgtaaaag aaattttcag
     3781 acatcccgaa tccgaagggt acggctttat caaagacagg agttgattta gtccctgtat
     3841 tctcactcca aagacttaga cttttaaacg ttcccacagc gctgaatttc attccaaagg
     3901 catccagatc tccgtcattg tcaatgtcag cataagtggc attcatcatt cccttcaaac
     3961 cgaaaggcct ttttttgggt gattcgaagg acggcaattg agatgttccg gaatttagta
     4021 aaaatacctg tccaaaatcg ctagcgccta aaaaatccat atctccgtca tagtcaatat
     4081 caacaacaca cagtagatag gtatttattg gtggactgat aagacctttt gtcttaatac
     4141 gcgttttttc aggattaaat tgctgcgcaa atgtctcatt aaacgccata gcgcatataa
     4201 acgaaaaaag tataaaaagc gctttcatat tttgaatccg gagtgaaaat agtgtctgtt
     4261 tcattttgga atatttttgc aaaaataaag cttttgtcag tttatttttt acctaccaca
     4321 tacatccacg caatcccacc cgataatttt tttgtccgga ctatctcaaa cccgcagtca
     4381 gtgaggattt tctccatgtc attcccatag gggaattgct gcacggtctg gttcagatat
     4441 gtgtaggcat tattgtggcg cgacaaacgg cggccacgca gcggaagaat atgattgaaa
     4501 taaaatttat aaacgtgtcg ccagaaaaat ccggttggca ttccgaattc aagtatcaca
     4561 aagtgcccgc ctggagcgag aatgcggtgc atctccttca gccctttttc aagcgatgca
     4621 aaattccgca caccgaaaga gcaggtgatg agatcgaagg cgttcgttgc aaatggggcc
     4681 atttcagcgt tgcatttcac caattcggcc ataacacagg atttgctgag ttttaatttc
     4741 gcaatctcca gcattttttc tgaaggatcc gctgctacca gtttttcggg atgtagcttc
     4801 gacagttcca gtgtcagatc gccggtaccg gccgacatat ccagaatctg ctgtggattg
     4861 tatttccgca ccgccttacg cagcttccgg cgccagatca catctacact gaaggaaagt
     4921 cgccggttga ttttatcgta ggttggtgca atgtcgttga acatgtcaac aacaacgttt
     4981 gatttttttt cttcggcttt cggatcgctc atcagccgcg gctttcggaa agtgatttca
     5041 ggaaccgttc ctgatcgacc ggcacaacgc ctacatattc gcacacaatg ccggctgcag
     5101 cattcgacca ttcggccatt tcctgcgggc tggctcctat ggccagtgcc agtgccgcca
     5161 ccgcaatcac cgtatcgccg gctccggaaa catcggccac tttgcgcaca cgcgttggca
     5221 tgtgaatgta gcccggtttc ccgttctcat ggtggcaaag cagcatccct cgctcaccca
     5281 gcgtcagcag cagatagcgg atgttttgtt gtttcctgaa ttcctcacaa aatgcagcca
     5341 gttcatccat ttcttccgga agctccttgt tcagtgcatc gcgcagttcc ttgaggtttg
     5401 gcttgaaaac ggtcacgttt ttataatgca gaaaattccg ctttttcgga tcgaccagca
     5461 ccggaatatt gtgtttcgat gcctctgaaa ccacagattc aatcagcttt gcatcaatca
     5521 cacccttgtc gtaatcttca aaaataatgg cgtcgaatcc gttggcaatt atcaggtcct
     5581 gcacatgctt tgtaaatttc tgctgcagtt cttcgggcag gggagcatca gtttcttcat
     5641 ccacacgcag catctgtaca ttgtttccaa tgatgcggaa tttggttgtc gtaatgcggt
     5701 catggctttt cagcagtccg ttgcggggca ttccgttttc agcaagcaga ttttcgaatt
     5761 gtgctgcttt gtcatcatcg cccaaaacgg cagaaagaat cgcagtacta tccagcgcgc
     5821 tgatgttcag agccacgttg gcggcgcctc ccatgcggct ttcgcgtttt tcgaccgata
     5881 ccaccggcac tggtgcctca ggactgatgc ggtccacctt gccccagatg taggcgtcga
     5941 gcatcacatc gccgatgatg agaattttct ttttctgaaa aggattttcc ctgaaattca
     6001 taggattgct ttttaccgta tgcatgaatc aactgcaaaa ataagaattt attgaggatc
     6061 gtgtttttgc ttgtagataa tgctatcatg ttgaactgag ctttttattt tcatggcccc
     6121 taatccgtct ccgccctata ctccgcgcgc gccggccgag tgtgcctgcg gtggccagcg
     6181 ggcttcctgc cggtcgccgc tgcccgacgg gcacacattc gtcccgcgct gcttgcgggg
     6241 tgccgggctc cgccggggtg catgccggat tgcaatgccg ggctgctgct atatattgta
     6301 tgctcagggt ttcacaccgg gctaatatat gccgcgcttt cagcgctttt tgtgctacgc
     6361 ttcgtctttt atgcctcggc atgatattgt ctcagttatg tctcagagct gatgactgac
     6421 tattccgtgc cttgtggacg ggacttcgcc gatggctgaa agcttaatgt aacgtctgta
     6481 acgtctttcg attccagcgg aataaattcc acgtctgtaa cgatttccgc ttccagcgga
     6541 ataaattcca cgattgtaac gtctgtaacg ccgaatactg aaatttaatt acatttgttt
     6601 gcttatttga acatgaaaaa gctgcattta cttgttttac gcgcctatgc cggccctctt
     6661 gtcctgactt tctgcattgc ggaattcgta ttgctgatgc agtttctgtg gaaatacatc
     6721 gatgaccttg ttggcaaagg gctcgatttt ctgattatcg gtcaactgct tttctatgca
     6781 tctgccacct ttgttcccat ggcgctccca ctggctattc tgctggcatc gctcatgacc
     6841 atgggaaatc tgggcgaaca ctatgaactt gttgcagcca aagcttccgg catttcattc
     6901 cggaaaatca tgatgccttt ggttttattg tctcttttca tcagtggtgt ggccttcttt
     6961 ttcagtaatc aggtgcttcc ggttgccaat ttgaaaatgt tttccttgct gtatgatgtc
     7021 aaggagcaga aaccggcgct gaatattcag gaaggcgtat tctaccgcga tattgacggc
     7081 tatgtgatta aagtaggaga aaaagataag gacggtcaga ccatccgaag ggtgttggtt
     7141 tatgaccaca caaagccggg cgggaattta tctgttacca ttgccgaaag cggtactatg
     7201 gttacaacag atgataagcg aacactgata tttacattat ataatggcac taattatact
     7261 gaaccgcgta ataatcgcga aagtctcacc agaagaccga tgcagcgtgt acattttagc
     7321 gaggaacagc ttcggttcga tctttcatct ttttccatga gtcgtaccag cgaagaattg
     7381 ttcaaggagc attatcagat gatgaatctc gaacagcttt caacatcgat tgatacgctg
     7441 gttactgagc gtggttcgcg gcaggacgag tttgcacacc aattgatgcg ttcatcgggg
     7501 tatttttaca attttattgt tttgcaaaaa cgtaaaccac tcgccggtaa tgattcgctg
     7561 gctgatgatg tatcccgcat tcgttttgat atgtcgccaa cccagtacag gactgccgta
     7621 ggcgaagcca taggaatggc acgcgccaat gcaatgatgg tggataatta caataaggaa
     7681 atggaagcca aacaaaaaat ccttataaaa cacgaggtag aaatacaacg taaattcact
     7741 ctttccattg cctgtttgtt gctgtttttt atcggagcac ctttgggagc tattattcgc
     7801 aaaggcggac tgggaatgcc attggttgtg tccgtcattg tatttatttt gtattatatc
     7861 atttccatta ccggcgaaaa aagtgtgaaa gaaggggctc tgtcacctga atttggcatg
     7921 tggatttctt ccatgattct tctgcccttt ggtatttggc tgacattaaa aaccacaacc
     7981 gacagccctt taatggaaac agattcctgg gttaagcttg ccgagaaatt caataaattt
     8041 ttcagaatca aaaagaagtc tgaatgagaa ttctgcaggt ctgtcataag tccccgtttc
     8101 cgccttccga aggtggtccc atggccatga aagctattcg tgacggattg ctggctgccg
     8161 gtcatcatgt gcatactttt acattgagta cgccaaagtt cactgtcagt gatgctatgg
     8221 ccaccaagat cagttatttc tctcattctt ttatcgatac ttcagtttat atctggcctg
     8281 ctttccgcga tttatgtctg aacagatcgc atcacgtttc aaggttttat aacgataaaa
     8341 ctgctgccag tttattgcag atacttcaga aatcgtcttt tgatgcggtt ttgatcgaaa
     8401 gtattttcat gatgccctat ttcaaaatga tcagaaaatt tttttccgga cccatcatat
     8461 tgcggtcaca taatgttgaa catctgatct gggaacgaat tgcagcaaat gaaaccaata
     8521 ttctcaaaaa aaaatatttg cagattcttt caaaacaact caggcgtttt gaaataaatt
     8581 gctttctgca ggccgatgct attgctgcca tcagcagtaa tgatgctgat tccgttcgcc
     8641 ggctcgctcc ggatgcagat gttgttccca ttccatttgc tattggtctt caaaacggac
     8701 caatccctca aaaatctttc tgtgctcgct ttggtcatat tggttcaatg gactgggctc
     8761 caaatctcga aggtatcgat tggtttctga attttgtatg gcctttggta ataaaaaaat
     8821 gtcctcaggc ttcattcttt ctggcggggc gcaatatgcc ctcgcgcttt gtcagtaacc
     8881 agtcgctcaa tctttttgtc gagggagaag tagaaagttc ttcgcaattt atgttgtcgc
     8941 tcgatgccct cattgtgcct ttgttttcag gaagtggtgt gcgtattaaa attgcagagg
     9001 ccttgtcgct cggagtgccg gttattacta ctcctgtcgg aagcgaggga ttgctggtta
     9061 aaaatggcag agatattatc atcagcgata atgcgctgca aatgacaacc gatatctgtc
     9121 gggtgattga taatcctgac ttattgcaaa atatttccga aaatggcttg aatactatac
     9181 gaaagcatca ttcatcggaa acaataacac aaaatctgat tgctttgatt gaacgaaaaa
     9241 tgaaatcatg aaccgcccga aattatttgt gatattgccc cggattccat ggccgcttga
     9301 aaaaggagac aaactcaggt cgtactactt tgtgaagggg ctgagcgaat attacgacat
     9361 tactttgttt gcgttgtatg atgagcgtta tagcaatgat gtgctcgaaa aaataaggga
     9421 gatttcggca gagtatcatt tttttcgtat tcatattttt tccgttttat ttcatgctat
     9481 attggcaata ttcacaggaa agccatttca ggtggcctat ttcaccaatt accggctcag
     9541 gcgtgccgtt cggaaagctg ctcagcagca gcaacccagg gctgttttct gccagttggt
     9601 gcgtacggcc caatatgcac gtggtctcga ttgtgtgaag attttggatt atcaggatac
     9661 tttatcgctc ggcatgaagc gccgggctgc ttcggctacg ggaatgcaaa aatggttttt
     9721 ccgttctgag ggaaaacggc tgatgcgtta cgaagctttg gtttctgact ggttcaatca
     9781 tatgctcatt atcaccgaac aggatcgcat ggagcttcct gtaagcgact caagggttca
     9841 tgtaattcca aatggagttg ataccgatta ttattcgccc gttgcatgcg acgaaattca
     9901 tgaaatgttg tttgtgggaa acatgtctta tccgcccaac atcaatgcga tggagtattt
     9961 tgtagattgt gttttgccag gtgtgctgga gcattttcca tcggcagaga ttgtcattgc
    10021 cggtgccagg ccacacagcc gcgtgaaaat actagcgtcg gatcatgtta ttgttaccgg
    10081 ctgggttgat gaccccagag attattatca gcgttccgct atatttgttg cacctatgca
    10141 aattggcact ggtttgcaga ataagttgct cgaagctatg gctatgcaac gcccgtgcat
    10201 aaccaccgat ctggccaaca atgcacttgg cgcccggcat ggtgatgaaa tactggtggc
    10261 tgaaagtctc gatgaatgga ttgaaatgat tgttgctttg ttgaaaaaca aagatgaacg
    10321 caaccgcatt gcaataaatg gcagaaattt cgttattgat aaatattcct ggcctgctca
    10381 aattaaaaaa cttcatcaaa tcatcgaatc atgaaagaca aatcactcat cagcacagta
    10441 attattgctt ttgcaattat tgttactggc tatctgcttg gaaccaacta caaaaaccgc
    10501 agccaaaaac cgcacacagt cgaagttacg ggacttgctg aaaagaattt cacatcggat
    10561 ttaattgttt ggaacggctc cttttcgaaa ttcaacaaag acatgaaact ggcttcgaat
    10621 gagctgaaaa aggatgttga aaaagtcagg gcttttttgg cagaacgcaa tatcccggaa
    10681 aatgaaatat tgttttcggc agttcaaatc aatcgcgaat acgaaacaac ctatgacgaa
    10741 gatggcaacc gtaaaagcga atttgttggt tatcaactga cgcaaaacgt gaaaatagaa
    10801 tcaaagaata tcaaactggt agaaaaggtt tcgcgcgatg tttcggcttt gattgatgaa
    10861 ggtgttgagt tttacagcga tgcgcctatg ttttattata ccaaattatc tgaccttaaa
    10921 ttgcagttga tagaggaagc taccaaggat ggtcgtgccc gtgccgataa aattgccgaa
    10981 aacagtggtg gcagcatcaa gcggcttatg cagggcaata tgggcgtttt tcaaattact
    11041 gcccgcaaca gcaatgaaga ttatagctgg ggcggggcat tcaatacctc atcgcttgaa
    11101 aaaacggcgt ccattacagt gcatcttgtt ttcgannnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnatgag cagttttcag ttgccggttg aatatgtcaa ggaaattccg
    11281 gctctgttgg gcgaaaagga tgtgtttaaa gctttgcagc ttttaccggg tgtgcagaag
    11341 ggaaatgagg gcagcagcgg tttgtatgtg cgtggtggtg gacctgatca gaatctgatt
    11401 attcttgatg atgctacggt ttacaacgcc aatcatctgt ttggcttttt cagcttattc
    11461 aacggtgatg cactcaaaag catcgaactt atcaaagggg gcttccctgc acgctatggc
    11521 ggacgtttgt cttcggttgt tgatatgaat atgaaggatg gaaatcgtga aaaattaagc
    11581 ggtgaaggcg gaattggcat tatttcctcg cgactcacac tcgaaggtcc tgtgataaaa
    11641 aacaaatctt cgtttctcgt ttctggccgc agaacttata tcgatgcttt gatgcgacct
    11701 tttatgaata aagataatgg cactttcggc tattatttct atgacctgaa tgctaaaatc
    11761 aattatgaat ttgacaaaaa aaaccgtttg tatctgagtg gctattttgg ccgcgataag
    11821 tttaattttg ccgaatcgat ggattttacc aacagtactt cgcgtttaaa agctggtttg
    11881 ttttgggaaa atgcaactgc tacgcttcgc tggaatcatg tgttttccaa taaggtcttt
    11941 tcgaacgctt cactcatttt cagcaattac ggcctcaata tttatgaaga ggaaaaatac
    12001 gatgattccc gttttagtct caattattca agtggaatcc gtgatcttgg tctgaaatat
    12061 gatttcactt tttaccccaa tacttatcac accctgcgat atggactgca gtcaacatgg
    12121 catcgtttca aaccatctgc agtggttttg gaaatgacga gtaccgatga attcgtttcg
    12181 gaaatcaatt caattcatac atttgagagt tcgctctata tcgaagatga atggaaatgg
    12241 aaccgatggc gtggcaatat tggcctcagg gcttttgatt ttatatccga ttcctgtcat
    12301 tatttcagcc ccgaaccgcg ttttgcagct ggttttatgc ttactgaact ttcgagcatc
    12361 aaggcctcgt ggacaatgat gaatcagagt gttcatctgc tcagcagcac aggaatgggg
    12421 ctcccaaccg atttgtgggt gccttcaaca aaaaatatca agccgcagaa agcatggcag
    12481 gccgcgttgg gctatgcccg cgatcttccc aaaggattag ctatgactat tgaggcctat
    12541 tacaaaaaaa gcgacgatgt aattggttac cgtgaaggcg ccagctttct gatgatcact
    12601 gatcctaccg atgccaacca gttcagctgg gaagacaata ttactgtagg tcagggatgg
    12661 tcttatggag gtgaattttt gctgcagaaa aaaaccggca aatggacggg atgggtgggt
    12721 tataccctga gctggactca acttcagttt gatgaattga atagcggaca gaaattttat
    12781 gcaaaatatg atcgtcgtca tgacatttcg attgtgaata tgtataaaat caatgatgtg
    12841 ttcactctga gttgtgtctg ggttttcagc tccggtaatg cattgaatct gccggttgag
    12901 acttatgatg ctgttgttga tcaaaacagc caggcagatt atatgtataa tttttacacg
    12961 tattactacg gaccgaaaaa tactttcaga ttaatgcctt atcatcgtct tgatattggc
    13021 ttgcagcatc atcagaaaag agaaaaatcg gttcacacat gggaaatcgg cttatacaac
    13081 gcatacaacc gccataatcc gtatttttat tttgttgaca gcggttataa tggcgaaaac
    13141 caattaaagc aggtttcgct ttttcccatg atcccttcgt tttcatacag cataaaattc
    13201 taaggatgaa aagaaaaaat ctgatatttg ttttactgcc ctttctgatg atttcgtgcg
    13261 aagatatggt cagtactgtc gatgttcccc aatccggctc gcattatgtg ctgcatgcat
    13321 atctgtcgcc cgaagattct ttggtgacag ttgttgccgg aaaatcaaaa ccggttttcg
    13381 gcgatcagac aactgatact tcatgggtat cgcagtctca tgttttagta aacggatttg
    13441 agctttcccg tgtgcctggc cccgattatc gttatacggc tccggctgcg tcttttcctg
    13501 ttattccagg tgaattttat acggttgagt tgaaagttgg caccgaaaca gtttgttccg
    13561 gttcttgcac tgtgcctgcc tggcgaaacg aatccatggc ttttgatggc gtcgattcag
    13621 tggcttatca atcctatgaa ggcacaattt attatgatta ttatattttg tattctttta
    13681 ccgatctggc ttctgaagag aatttttacc gtattgccgc agaagtaacc tatgtcgatc
    13741 cttattccgg cgatacgtct gttttcacaa cctatcccga gagctctcag tttataaagg
    13801 atgttctttt caacggcgat cagtattctg agcggatatt tcttggcgga acagaggcgg
    13861 tgagcgggct tgcaggtctc cggctgatgc tttatacaag cgacagggat tattatttct
    13921 atcacagggc tattgtgtca caggatggcg atgatccttt ttcggagccg gttaccgtcc
    13981 cctcaaatgt cgataatggt cttggctgca ttgctggtgt acggaaatac actcttatcg
    14041 ttttttgatt ctttattcta ctcctgtccg atttgcggct gttgtatcgg ccgaattgtg
    14101 tatcaatcgc tttaagctta tgattgatgc gcatgctcct gaaattatgt gttatgaaaa
    14161 tcaaaaagat tgtatttgta tttgattttt ttaatatttt tacttcaaat tcgggtacat
    14221 gaaaaaaatt gtttttttat tgttggcagt tttaattttg tcttctctgc cggatttttc
    14281 gtttgcacaa aaccccaata agattgcaga acgcgaatgg aaacttttgt ttgaagaatc
    14341 aattgaatgg tataactttg gtttttttga aaatgcgctc aggggattca aacgtttgct
    14401 ggtgaaagac cgtaataatt gcaacattaa tttttatatt gccatgagtt attattatct
    14461 caggcgtccg gccgatgtta ttattcctta tatggaaaaa gcatcgaaaa aagttgaccc
    14521 ttattatgga tatacctata aggaaacttc ggctcccgta ttttcgcttt tgtatctcgg
    14581 acaattgtat atgaatgtgt atgaatttga taaggccgac actgcattta tgcgctttgg
    14641 catgtatctg accgataaaa accgcgatgc tgcttacctg atggaactgt cgctctggat
    14701 gaattatgtc aataatgccc gcacccaata tgccaatcag ctgaaaaatg tcagtgttga
    14761 aaacattaag ctgattaact ctggttataa cgaaggtcag ccttttctgg ctccggacgg
    14821 gaaaaccttg tttctcacat ctgacaggaa gggctctacc ggcggacaat ggctcaacaa
    14881 tgtgtataag tccgatgttt acatggtcgc tataaaaaat ggccggatgt ataaacccaa
    14941 aaaaatgggt cagaaaatga atacttctgc tgctgatatg tgcaacagca tgggcgaggg
    15001 tggcaagtat ttgctcatta gccgcgaaga caagaacaat aaggattaca acatttattc
    15061 atccgaaatc agcggaaaac gtttcaaaga gccgcaaaaa cttaatccaa acatcaatag
    15121 caaagccaat gaagttcatg ctttcgtttc gctcaatggc aatgttatgt atttttcaag
    15181 cgaccggccg ggtggctatg gtggccttga tatctatatg tcggaaaaaa tgcccaacgg
    15241 ggaatggggc cctgcttata acctcggacc gactgtcaat acctggtttg atgaagatta
    15301 tccttttgtt ctcgatgatg gtgttacctt gttttttagt tccaattgca ctaaaagtat
    15361 gggtggcttt gatatttttg tttctaccct ttccgaagaa ggaatgtggt cggatccgga
    15421 aaacatcgga tatcccatca atactacgtc tgacgatacc ggctttatga tgacccgtga
    15481 cggattaacc ggttattatg cgactgcgcg tgaagctcag tccaatggac atatcgacaa
    15541 tatcgacatt tactgtgttc atttcggaaa atagattatg ctgagaaaag tcttaactgc
    15601 ctttttgctc atcgcctcta tgtgtagtcc tgttttatca cagactggat atgcgctcaa
    15661 agagaaagat gcatatgcct cctgtgttga ttttattatt gttgacgatt ccggcaaaac
    15721 ggtggatgtt ccggttgcag taagccgcgc actccaatgc ccgtcattgc tttcgctgca
    15781 tggagatact ctttgttacc gttccggaaa ttcaatccgt atgtatcata tttcttcagg
    15841 tgctgattac caactgttcg atgttttcga tgatgttgat ggtgtctcgg gtccggtttg
    15901 gtcgccgtcg cacaggcgga ttggctttgt catcattaat cagcagcgct cgcatggtta
    15961 taaagatatt tgcagaatca ttattcttga tcttgatgca gatttcaaag tgaaatttat
    16021 gcacaggtat gaccgtcctg tcaatttcag ttgtggcagc atctgttcct ccgatgcagg
    16081 atttgatttt cgttttgcag atgaaaataa ttttgaatat atccgtaata tcaatattga
    16141 agatagcccc ggtgaaaaag gatacatttt tattggaaat tgaatttggc gcggcgtaat
    16201 agcagtcaat agttcaatga tattttgatt accggatttt ctcgaagaga attttttaaa
    16261 tacctttgtc tgaaaaaatc aaacaaaatg tttcaaacaa aatgtttcaa annnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaaacaaa atgtttcaaa caaaatgttt
    16441 caaaaaagtt gtatatttgt ggaaattttg cgaccatgaa ttccgaattt aatcccttcc
    16501 cggtttcagg atattatgga gctgactatt tctgtgatcg tgacagcgaa acaaatctga
    16561 tccgccggaa tatccgtaac aacataaaca caaccttgtt ttctgtccgt cgcctcggga
    16621 aaaccgggct cattcatcat ataattgagt cctattcaaa ggacaggcag gtcgtttgtc
    16681 tttatattga cattttcagc agcacctgcc tgatggattt taccaatatg ctggcaacag
    16741 taattttcaa tcgctttcct gaaaacaata caatcgggaa aaaagtcatg aaggccattg
    16801 cttcattgcg ccctgttatt agctacgaca ccctttccgg ggatccggag gtgcgtttcg
    16861 aatatatggg cgcatcgcag tatgaaaaaa cgctgcagca gcttttttca tttcttgacg
    16921 gacagggaaa gaaaatcgta tttgccatcg atgagttcca gcaaattctc gaatatccgg
    16981 agaaaaacat ggaagctgtt ttgagaacac atattcagag attgaagaat acctttttta
    17041 tattttcagg cagcaatcag aagatgatgc acgaaatttt caacaatcag aaacgacctt
    17101 tctatgccag ctgcagcaat gtcaatcttg ggtttattga tgaaaacatt tatgctgatt
    17161 ttattcgtca gaaattcaag caacataagc gcagtataag tgatgagtgt atctggttta
    17221 ttcttgagtt tacacaccgg catacatttt acacacaata tttctgtaat catttgtttg
    17281 caagtgggat taagcgtatt gtgaaaaatg atgtttatgc tgcagcaata gaaattctga
    17341 agctgaatga gagtacttgt tttcaatacc ggagtttgct caccaaggca cagtggaaac
    17401 tcctgacagc gctcgccaag gaggaaaggt tgcaaaaggc gcactcagtt tcgtttttga
    17461 aaaagtattt attgggtacc tcagcgcatg ttaccagagg aatggctgct ttgattgaaa
    17521 aggatctggt ttaccacaac acttctgtag agcagcccta ttataccatc tacgacaaat
    17581 tccttatgcg ctggctgcag cgtttgtgaa cgttttcgta ctatcagtat aaaacctgat
    17641 agctgataat catcatggtt tgccatttgc aaaattctgc aaatcgctgt acttttgtca
    17701 aaatttcgca gatgatttct tttgaagaag cttatgacat tgtcagttcc caacctgtta
    17761 ttgccggaac agaaactatc cggtttgata tggcttcagg tcgtattctt gccgaagatg
    17821 tgtgctcgga catggaaatg cctccgtttg ataaatcggc ggtagatggc tttgcatgcc
    17881 gaattgatga tattagtcag cccatgaaaa ttattgaaac agtgccggct ggcaatatgc
    17941 cgtcgatgca ggttgttgaa ggaacttgca tccgcataat gaccggagct cctgttcccg
    18001 atggtgccga tatggttctg atggtcgagc attgcgatgt ggctgatgaa gttgtcttat
    18061 acaagggaac atccaggaac agtaacattg cctatcgcgg cgaagacatg aaaacaggag
    18121 cgctcctgat tggtgcatca actcttgtaa gtccgcagca tattgccgtc atggctgcag
    18181 ttggaaaaac tgaaattaag gtgttcagtc aaatcagggc tggggttatg agtaccggta
    18241 gcgagcttgt ggagccggat ttcaaaccat cgcttgcaca aatccgcaac agtaattcat
    18301 ggcagctgat ggctcagatt acgtctcatg gcggcattcc gaagtattat ggtattattc
    18361 cggatgattt tgatcaaaca ctgttggcgc tgaaaaaagc tattgaggac aatgatattg
    18421 tgtttttaag cggcggggtt tcgatgggtg aattcgattt tattcctgct gcattgcgtg
    18481 aagcaggatt tgaaattttg tttgatagtg ttgccgtgca accgggaaag cccactacac
    18541 tggcgcgaag ggataacaag tatattgtgg gattgcccgg aaatcccgtg tcttcttttg
    18601 ttcaattcga gttattgggt tctgttctaa tgaaccgcat tggtggtgga ataaaagaca
    18661 ctgttgttgt tcgggcaaaa acaaacattg ccttcaatcg caaaaaagct gaacgcaagt
    18721 cgttttatcc tgtgtgtttt aatgcaaatg gttatgttgc atctcttgaa tatcatggat
    18781 ctgctcacat caattcttta ctcaatgcgg ttggtgtggt tgccatggat attggtgtga
    18841 aagaaattcc tgctgaaacc gaagtagatg ttcgactgtt ttaatcgtca tataacttat
    18901 ctgcgcgtgt cggtaaccga tcgctgtaat ctgcgctgca cctattgtat gcctgcagaa
    18961 ggtgttgttt tgctttctca caatgacata ttgtcgttta acgaaattgc cgatgtggtg
    19021 cgctatggcg cagagcatgg aattaaaaaa gttcgtatca ccggaggaga accacttgtg
    19081 cgtaaaggtg ttgtacagct ggttgaaatg ctttctgcta ttgatggaat tgaagaaatg
    19141 accatgacct cgaatgggat tctgctccct gtttttgcca gagatctcaa gaacgctggt
    19201 ttgcatagaa tcaacatcag tctcgataca ctgaatcctg aaaaatatgc ttcaatcaca
    19261 cgtggcggaa atctgaatga tgttattgcc ggaattgatg cggctattga agctgatctg
    19321 actccggtta aactgaattg tgttatccga aattcgccat cagagcccga tgcacaaatg
    19381 gtgaaacatt ttgctgatag tcgtggcatt gaagtgaggt ttatccatct gatgaatctc
    19441 aataccggcg aattcagtaa agtaattggt ggtgacggag gtaattgcgc aatgtgcaat
    19501 cgtttgcggc tgactgccaa tggaaatctg attccatgtt tgttcagcaa cgaagaattc
    19561 aatatccgga aactgggaat agctaaggcc tatgagttgg cattgcaaaa taaaccaaag
    19621 tccgggacta catcaaatac gcatggattc tataataccg gaggatgatt tttgatgtga
    19681 tgatttgata atgtgctgat ttgaaaattt gaaaatgaac ttgtcatact tgatactcct
    19741 tgtcaccact gtcacgcttg taacgactgt aaagtgtaac gactgtaacg actgtaaagt
    19801 gtaacgaaat gatgtgaaaa tgtggagatg tgaaaatgtg tgaagagaga ttagttttga
    19861 attcagtaac gcctgtaact ttgttacgtg taacgactgt atcgactgta acgtgtaacg
    19921 actgtaacgt gtaacgaaaa atgaataaaa cgaaatattt aatcggaatc gatgacaccg
    19981 ataacctcac cagccgcggc acgggattta ttgtgcgtga gcttgcacgt cagttgaggg
    20041 aagctgggat tgcataccca cattgcgttt cgagacatca gttgtttttt caccctgata
    20101 ttccctatac ttcgcataac agttccgctt gtcttgaatt ggattcggaa aaaccggacg
    20161 agttgaaaac atattgccgc acttatttgt tgaatgtggc tgccgaaggt tccgatgccg
    20221 gtttgtgtat tgtggctttc gatgaagtgt cggaagagca gattcagtgg gggcttcgtg
    20281 ccaaaaaaga gattcttaca aaagatgccg cccaggatct ggcagcacgc aatcatgttt
    20341 ttctcgaagg attgactggt actcgcgatg gcatcatcgg atcgttggct gcctgcggtt
    20401 tgcgcaaatt tggctgtgat gggcgtattc taatgctggc tgccgatttc aggtcttttc
    20461 agggttccgc aacagccgct cagatatgca caaattttca tattcggaat atttttgata
    20521 tttttgatgc cccgattccc cctgatgatc ttattacttt gactgaaggg tggaagccgg
    20581 ttctcagaaa tggcatatac actttaattg tggaaaaaga tggcgaacac tcttggaaat
    20641 caatcaataa agactatctc aggtcaatct cgtcgtgata ttgcactggc aattcagctt
    20701 ttaattttgt ttgctgctgg ttttggtgcg gtcgttcttc atgcacgatt ccgcaatcct
    20761 atcgggattc ccggccatca tgggttggaa tttatggcgt tggctgttac aacagctctg
    20821 tttttccgtt ttcgcctgag aggtgttgtt ttttctttgg gcgccggcgc agcattgttc
    20881 ctgccttttc ttggatttac tgaccctttt gccgaattcg tttttatgct tcccgctatt
    20941 attttgtgct tgcttgcaga tgcaattaaa gtgagtggca aagctgccac atttgtttta
    21001 atggctctcg gcggactcgc gtatatgagc attcccctgg ctcggttagc cattcatgca
    21061 gttaccggtt tcccatataa atctcttctt gtaagccctt tttatgttgt tttcatgcat
    21121 tttgtattcg gtgctgccgg tgctttcctt ggctatctga tttatcgggg actcaaacgt
    21181 tcctgacttt ttttattctg cgatatgaaa aaaaacacaa gacctttcat ctttttgatt
    21241 tttctggcag gcctcatgtt ttcaatgatg atgtcgtgca ctccggatga aaatcccgga
    21301 ttgcctcctg ttttgaaagt tacatttgcc gacagcacct ctgtttccga caccagtatt
    21361 gaagccgggc agagcattga cattatagtt gaaggactgg gcaatgaaaa caacattact
    21421 tatttttcat tgatacgtaa tggggtacat gtgctagaca gcggcttaaa cgctccagga
    21481 tttgtgtcgc gcagaaccat tgtacgaggt accgacagca tagagaatta cacagttttg
    21541 attcgcgacc gtaatttcaa cgaaacgact tttgatttca gtatcggcct taaaccaaca
    21601 ctggtttatg ggggtatccg ccattgggcg catgttgtat tgggtgctca atcaaattct
    21661 gttaccggaa gtttttttga tttattttca ggacagattt tcagtcttgc acaggccttt
    21721 gtcaatcagg attctgttca gctggtttat ttttacgatg ctgtcgatct gaataccatt
    21781 gcatcgccta acgcaaatat cgatacctcg tattttggcg gtagcagcgg gctcgccaac
    21841 tggactgtaa aaaatgaagt ccgatatatc cagattttca atacagaaca ggagttttcc
    21901 gattgccaga atgattcact gattattgcc aatatattcc cttatgcaac cggaaagcga
    21961 aaagcaaaag ttttgcagcc aggccacatg tatgaatttt ctttcgctgg tctttacggt
    22021 attttttatg ttcacaatgt aattggaacc gatgccggaa caatagacat cagtattaaa
    22081 atgcagcaac catgagacaa tttgtatttt ttatttttgg attactgatg tcattgcagc
    22141 ttttttcgca acagactttt actgctattg tgcgtgatgc cggcacgggt aaagccattg
    22201 aaggtgtaac catcactgtt gacagcattc gattgggagc catcagcgga aataagggag
    22261 agtttaaact ccggctgccc ggaaaagggc catgggtgct ccgtttacgt catattgcct
    22321 atgaaaatac agcttggaat gttcgtaaaa tatcggatag tcttgaaatt catgagatca
    22381 tttatttgcg tcaggcaccg catcaaatca atccagtagt gattacatcc gatcgggttg
    22441 agaaaagcac ggaagaagtt cccgctcgca tgaatgtcat aaattccact tccattgaga
    22501 gttcgccatc aaccaatatt gatgaggttt tgcgttcggt gccgggtatg tatgtcaatc
    22561 gcagttgggg tatgtattcg cgcaattcca gtgtaaccat gcgcggaatg ggtagtgcat
    22621 cacgtgtatt ggtgatgctc gacggtgtgc cgcttaataa atcgggtggt ggtggagtga
    22681 attggaatct tcttcccaac ggagcttttg aaaaaattga agttgaaaag ggcccgggat
    22741 caagcatgta tggcaataat gccatgaccg gaaccatcaa tctgatcacc ggaagtcctt
    22801 ccgaaaaaga aagagctgaa gcagattttt ttgtttcgca gtttaatact cagggcattg
    22861 gatttcggtt tatgacgcca tccaaagcca ggaaaccgta tgtgtggctc aatatttctt
    22921 caatgcaggg cgatggttat tttctggatt ctgatgaaac acgtaatgaa tattcttcga
    22981 agtgttttct taatcaatac aatgcttatc tgaaggccgg ttatcttatc agcgatagcg
    23041 cacgatttga tgtatcagtg ctgggttccg gtttcaaatc tggcttgggt aagtcggttt
    23101 ttgaagtcaa tggaaattat gatgatttca gaacatggat gggaagcgcc cgctacaagg
    23161 ttgtgagaaa aaagaataca tggaacgctc tggtttttgc caattacgaa gattatttta
    23221 atcaaagtga aagcatcaat gattatggcg aatacaaatt atccgaaaat ccaacagaaa
    23281 aactggatgc cggtgtgtgg acaaccatgg agcatcgctt cagtaaagat ttacagatga
    23341 taggtggcat tgattgcaag tattctgtcg aagatggcga aaatatttat ctcactgcta
    23401 cggatgaaat ttatttttat ggggttcata attttgctgc ggcttttgtt caggtatcga
    23461 aaaaactatg gagccgttct ttgctaaaag cgggattcag atatgatttc gggcaatata
    23521 atcagggtga aattctgatt gaaaatgcta cagcaatcag cgatttttta cttccttacg
    23581 caggtcagca tccttcaagt acctggaata gtctcagccc caaactggct tataagtttc
    23641 tgatgaatga aaaattcaac gtctatgctt cggtttccaa aggttttatg ccgcctacaa
    23701 tcgatgatat gacgcgttcc ggtaaaatcc gcaaaggaat aaaaattgcc aacccttttc
    23761 tggaacctga ggtgttgtat aatttcgaaa cgggatgtca gtggaaacca aaggaaaact
    23821 ggacgataga accggctgtg tattattcta ttgctgacaa tatgatttat caggtttgga
    23881 ccggcgaaga ggttgatctg atcggagatg gccccaaacc tttgattcag aagcgtaatg
    23941 tttcgcaggg aactgttgcc ggtgccgaaa tttccatcaa ctggaccccc cgaaattggc
    24001 tcaatgtgat gtcggcctac gcattcaacg attcacggat ttcggaatac agctctttgc
    24061 ccggtgatgc cgacctgacc ggattgttta tggctgaagt cccaaaacat ctcgcctcga
    24121 ttgatatcag ggcgcaatat cgaattttta cgcttaccac tgagtatcgc tataccggtg
    24181 aaacttttgc cgatgacgaa aatgttgata ttattgatga ttaccatgtc gtgaatatga
    24241 ctctggctgc aagaattaaa aattacacca tcggtatttc ggtcaatgat ttgctcgata
    24301 ctcagtttat cgacaagaaa ggttatctga gcccgggacg tttcatctcg gctcgtgtca
    24361 atgtaaaact ttaaagagaa agaaccataa aaattgattc aggtaattga tttttgttac
    24421 atttgcatcc gttatgcaac aaaatgcata acaagttgaa aatgagaaca caaattattt
    24481 tatttacact ggtgttgatg gcattgtttt catcatgtaa aaaagatgat gattcaacca
    24541 cacctccgct cctgcatttg caactgggtg gggcttatgt ttccaataac gctgtgctgg
    24601 ccatcaacga caccatttct tttggggtgc atgctgaagg tgtatcttca aacctgacgt
    24661 atttcaaagt ggatgtaatg agtgaaaatg gcaccagcac tatttatgat gaaggcatga
    24721 atcgtaaaac atttgatgca actaaaatat ttttcaaagg tattccggac gaagagactt
    24781 ttgttatcac tgtgatggat tacaaccgca atacaaccag tgcctcattc actgtttttc
    24841 gtgatacact gagtgggttt ggtccgattt atcatttcac gaatcttgta cttggctacc
    24901 agaacaactc tgccagcggg catttccttg atccgaacac cggaattatt tacgatgatg
    24961 caacggtaag cggccatgag agcgaggttg acattgttgt ttatttctat ctcagcagcg
    25021 gatctccatc accgacactc gtttgtccgg cacagaccga tgcgcaggct caatatccgt
    25081 caatggcggg gtggcctgtt cagaatgcaa cattgtatga ttatcacaca tcggattacg
    25141 atctgatttc tgctgcacaa tttgatgaat gtaccagcga tagtttgctt attgctgcgt
    25201 ataatcccac atatgtgaac cagaaatgca agtttgcact gactgggaaa attgttccgt
    25261 ttcttacagc agagggaaaa aaaggactga taaaggttgt tgcagccgat caggccgaaa
    25321 caggtaccat gacgattgaa attaaaatcc aacaataaaa aaaacagaaa catgaaaaaa
    25381 tctttacttc ttttcgttgc catgcttgga ataagcatgt tcagttttgc acaacttgac
    25441 accgtgatcg ggttcacttt tccggttgaa gtcggccttg acagccttga tgcaaccatc
    25501 ggagatacgc tgaatattgg atacgatatt cgttttgaat cggaatcggc cggaacaact
    25561 gattcagttt ttctcaccaa tggtgcaact acttatgccg ccaccgcaaa cagctgggaa
    25621 gacggtatga acgataaatt tttctccatc aaattcaaaa ccggttatta caaaaatctg
    25681 gttttatatg caaaaatgcg cagtggcggc actaatgccg gaccgaaata tttcaaggtt
    25741 cagtataaaa tcggcagctc aggaacatgg actgatgtca gcactgatac ggttacagtt
    25801 gccaacgact ggactaccgg cgttgttgat ggatggaatc ttccttctga aacatggaat
    25861 gcatctcagt cggtgtatgt tcgttttctg agcataacca acaccaatgt ttccggcggt
    25921 actgttgatt cattgggtgt cgtgaaaatt gacgatattt ttgttcttgg cgaaaacaac
    25981 accggtgttg gtgaaaacag cctgatgaat acccgtgttt atcctaatcc tgccaatgat
    26041 gtgctgaaca ttgaaaacaa tgacaatgtt tccgttttct ttattatcag ctcagatgga
    26101 cgcgttgtat ctcagatcaa caatccggca acaagcattg atatcagcaa tctggctgca
    26161 ggtcattata tgatccgcat gatctctgat gacaactcaa tgaagacaca aagtctgatt
    26221 gtagaataaa ttgaaatagc atccttcgaa gggtttcaaa cccttcgaag gatgctatca
    26281 aagtcgaaaa tgaaaaaact tgttctgatt ttaattgttt tgtttccctt gttatgtgca
    26341 ggacagtctg ttctttacgg gtatgtattc aatgcatccg ataaaaaggc gattgctgaa
    26401 gcttcggttc gatggataaa aaacaagaca ggaacaacca caaaatcaga cggaactttt
    26461 tcaatcacat attcttcgat tcaggacgac acgctcgaaa tttcgcatat aggatatgtc
    26521 attctgcgca aagcttcttc tgaattgttt tcggaaaatg caaaaatatt ttcatttgcg
    26581 ttgacaacgc ttgcatacaa tgtatcacct gtatgtatta ctgcaacgcg tgaagaatgc
    26641 gatccaatgc agattccgca aaaagtaaca ttgattgatt cggcttcatt gcatgcattg
    26701 cctctgcaaa gcattgacga tgcatttatt tactcttcgg gtgtaatgat cgatcgtccc
    26761 atgggtattt tcgcggccaa atccattgtt accatgcgcg gattatctgg cagcgagcag
    26821 ggtagaacac tggtgctttt ggatggaatc cccatcaata aatcggatgg cggcaccgtg
    26881 aatttcaatc tggtcgatcc gttgctggtc gatcgcatgg aaattgtgaa aggcccttca
    26941 tcggcgctgt atggcggaaa tgccatgggc ggtgtaatta atattatctc tgcaattcct
    27001 gaagaaaagc tggccggacg tgtgaaactc ggctacagca cgttcaatac cctttccgga
    27061 gacatggctg tcaacagctt tctcggcaaa acggctgacc gtggcttttt ctgctccggc
    27121 agcgcattct ggcgcgaaag cgacggctac atcagtgagc cggattacat gcggaccgaa
    27181 tacaccgtgc ctgcatttct gaaagaaaaa atgttttcgc tgaaaggcgg ttataaattc
    27241 aatgcaaaaa gtcatttctg gatcagcgga atgttgttcg acgatgaacg gggcggggga
    27301 gaaaaagtgt acgaagaact gggctctttt tcggaacacg acagttatca catacgcagc
    27361 cactacagaa ctgaagccgg gaaatggaaa ctgaccgcca gtggttttta tctgcgcgag
    27421 aattactcaa aaatcaatga gtatgtaaaa gctgatgaat acacactcta cgatgtcgat
    27481 tcacgcagaa cggatattgg aatcattgct catgggagca gggaaattgg aaaagcaaac
    27541 agactcacca cgggatttga agtgcgtcag ggcgctgtgg atgcggctga tgtatatcat
    27601 acgtcaaccg acaaaatcat caacgcaggc atcatggata tgctggcgct gtttgcccag
    27661 gatgaattcg ttttcggaaa agagcgctgg aagctggtta caggacttcg ttacgattat
    27721 gccggttttc acgatggccg cttttcaatc gaaaatccgg gaatgaataa tatgtatttg
    27781 ttgttatacg aaaatgacaa catggctgaa aagcaatggc attcgctgag tccgaaggtg
    27841 gcactgcagt tcagaatgaa agaaaacaaa cggatttata tttcatatgc tcatggtttc
    27901 cgtgcaccga ttctcgatga cctctgccgc tcaggcaaga cccggggcgg atttcaagtg
    27961 gccaattccg atcttgttcc ggagactgta gagaattttg aaatcggata tcgacacgaa
    28021 atcaattcaa aaatcacggt tgaaccggtc atatattata gcatcggaaa taatttcatg
    28081 tatccggttg caaccggaga tacggtcgac atgggctatg cagcgccggt cattgttacg
    28141 tcgaatgtct cagaagtccg gattgccggt gccgaaatcg atttcaccta ccggataaat
    28201 aaaaacctga atgcattttt taattatgcg tttgcgcatt caacagtaac ggaatacact
    28261 cctgcaatgg cgcagcagat cgatatttcg ggattgtatc tcagcaatgt tccgacgcat
    28321 ctgaccaatg ccggaattca atataggtac aaaaacgcag gcctttcttt caatacgcgc
    28381 tatgtgggat cgcgctgggt gaacgacacc aataccccgg acgaaaaata cggtctgcct
    28441 gccgaatacg acgcttacat tacagccgat gtgaaagctt cgtacaccta taaaaaacgc
    28501 tggattgtat cggcttcggt cgtcaatatt gccgatgaac tctacaccga cagcaaaggc
    28561 cagtactgcc ccgggcggat gatttcattt gatgcgggga ttaagttttg agtactaagt
    28621 tcccggcccc cgcgccggga tctcctcgaa agccttcgcc acatctccac ttcttcctcc
    28681 gcgaatttca ctaattaacg cgaagacctc agcaggtctt atttgcgcaa atgcgaatca
    28741 gccttcacct gcggtgaagt ccgattcagt ggcgtactaa gttcccggcc cccgcgccgg
    28801 gatctcctgg aaaaccttcg ccgcatctcc acttttttgt ccacgaatta cactaattcc
    28861 acgaattagc tccttcgcct gatttaagcg aattatcacg aacgaattaa ccttcatctg
    28921 cgatgaagat taattcaggt ttgaacaata tattccgcgt ccgccgcgtt tatccgcgtc
    28981 taattccccc ctggccacac cccgcaccca aagcccaaaa ccaccagccc ccacacccgc
    29041 acagcccacc aacagcgccc aaaaacccct cctgcgccca cagcgcccat tgatagcact
    29101 ttccagcccc ctcatcgaat aaatttcaaa attatttcgt tataatcgct tcttttaact
    29161 aaatagttta tatatttggc ggtgagagat gggtgggggg attctgatgt gagatattaa
    29221 aaactaacgc aagctaattt gaaaacctaa aagttttttt gaaagaagca tcaattttta
    29281 tcagtagtat aatctggtaa ttcagacaga taagcttata aataatgaat acaatgtttg
    29341 ggaataaaat taccgctgaa ataattgctc gaatatttca aagagcagat gaggtagcaa
    29401 gaaatgaaat tgtgaatgga gctgttaacg atattaaaga acgggatcgt gtttcagttt
    29461 tatgtcgtga aatcagaaga aatattaata aaaatccatt tatccgttcc ttatccatct
    29521 cagctgaagc tgttgataat aaaaaaggag atgcattgtt cgtatttcga tataaaaatg
    29581 aagtaaaaat tggattagtt gaggcgaaaa tactaaaaat caataatgca aatttggatg
    29641 cttattttga ttggattccc aataaacata actattctca ttttactcat caactgaaaa
    29701 atttgcaaaa gtgggtaaat gaaatggctg tttggaatat gtttattcct aattgtccaa
    29761 atggccaata ttcgccaccc ctcattgcta aaggatcttc gaatatctgg agctctgacc
    29821 taattaatca cccgaaagtt agcactccgt tagtccgttg gaatttcaat gatgctctta
    29881 acccacctgt gcaatacatg aatttacata ccgtaatcaa atcaatttta aattgtaaaa
    29941 acggaaaagt tcacattgtt tcacaaggtg gacgggtaga ggttaaggca ttagatgatg
    30001 aaataataga tattccggta ccccattttt taggaagtat ttggggtgat ttaaaacagt
    30061 tttctcggaa atattacaac atcaattctt ataactatta tagatttgat gatcttgttg
    30121 aagtagttga tgattttaaa gaaaagcgag tattgcttaa agacccattt attaaaaggg
    30181 aacattttga tgaagacgaa tacatttttt ttaaacaaat tctaaatgaa ataataaaat
    30241 aacgattaca attcatgaat aaaaactaga tctggatcaa taagcaatca aatattgtgg
    30301 ttagttgcca ctaatatttc cattgaaaaa tcatgcataa aatcactaaa gacctcacac
    30361 ccccaaaaca tttccaccac cccaaacaaa aaaattgcga actttgatac cggaatacat
    30421 tttttaaaat aacaaatggc aaaacaaatc aaggataaag caattgaggt aacactgtgg
    30481 gatgcggcca acaagctgcg cggcagtgtg gagccggctg aatacaagca cgtggtattg
    30541 ggacttattt ttctgaagtt tgcaagtgac aaatttgaag aacaccgggc cagactgatt
    30601 gccgatggca aagaaaaata cattgagatt gccgactttt acaatatgag caatgtgttt
    30661 tttctggaag aaacaagccg gtggagttat ctgatcaaaa aatcgaagca gaatgacatt
    30721 gcattgctca ttgatacagc gctgcacacc attgaaaaaa acaataaggc actgaaaggt
    30781 gcgttgcccg ataattattt ttcgcggctg gggctggatg taaccaagct tgcatcgttg
    30841 ctcgatacca taaacgatat tgacaccaca cgcgatccga agcaggatct ggtgggcaaa
    30901 gtgtatgaat actttctttc gaaatttgcg ctggccgaag gaaagggtaa aggcgaattt
    30961 tacacaccca agagcattgt aaacctgatt accgaaatga ttgagcccta caagggcatt
    31021 atctatgacc ctgcatgtgg ctcgggcggc atgtttgttc agagtatcaa atttattgaa
    31081 gcacatcacg gcaacaaaaa agaaatttcc atttacgggc aggaatatac caacaccacg
    31141 tataaactgg caaagatgaa cctggccata cgtggcatca gtggcaacct gggcgataaa
    31201 gcggctgaca cttttttaga tgatcagcac aaagacctga aagccgattt tattatggcc
    31261 aacccgcctt ttaaccagaa agactggcgg gccgaaaacg aattgattga tgacccacgc
    31321 tggcgcggat acgatgtgcc gcccaaaagc aacgccaact atgcctggat attgaacatg
    31381 gtgagcaagc tttccgaaaa cggcgttgca ggcttcattc tggcaaacgg tgctctgagt
    31441 ggcggcggcg aagaatacaa aatacgccgc aagctcattg aaaataatct ggtagaagcc
    31501 attcttgttt tgccgcagga tatgttttac accaccaata tcagcgttac gctttggatt
    31561 atcaataaaa acaagaaaga acaaaacaaa acagtaggtg acgaaacccg ccattaccgc
    31621 gaccgtgcaa cagaaatcct gtttatggat ctgcggcaaa tgggtattcc atttgaaaaa
    31681 aaatacacgc agttttcgga agaagatatt ttaaaaatta caggcacata tcaccaatgg
    31741 caggtttcga ctccgctcaa cctgacaggc tatgaaaata ttcctgagtt ctgttattcc
    31801 gcttcgtttg atgaagtggc caaaaaggat ttttcgctgg tgcccagcaa atacattgag
    31861 tttgtaaacc gcgacgagaa tattgatttt gatgaaaaga tgaaagcgat tcaaggtgag
    31921 ttggcagatc tgctgaaagc cgaggcatca tcaaaagatg atttactaac cgtgttcaaa
    31981 gaattgggtt atgaaatcaa attttaagcg aatcggcgat tttatccggc tggtggacga
    32041 gcggaacaaa ggactgcacg taaagcagct gcttggtttg agtatttcga aagagtttat
    32101 tccttcagtg gccaatatta ttggcaccga catggagaac tataaaatta tccgcaaaaa
    32161 tcaatttgcc tgtagcacaa tgcaggtgcg gcgtgacaag aaaatgcctg tagcattact
    32221 gcagaattca gatgaagcca ttatttcgca ggcatatccg gtttttgaag taaaagacga
    32281 aaaggaactt ttgcccgaat atttaatgat gtggttttca cgctcagagt ttgaccgtga
    32341 ggcctgtttt aatgcagttg gtggagtcag aggcagcctt gagtgggaag attttgaaaa
    32401 tatgcaatta cccattcctc accccgataa acagcgcgaa acagtagccg aatacaacat
    32461 catccaaaac cgcataaaac tgaacgaaca gctgattcag aaactggagg aaacggcgca
    32521 ggcggtttat cgggagtggt ttgtggagtt tgaatttcct gatgaaaacg gtaagccgta
    32581 taaaagtaat ggcggggaga tggtttggtg tgaggaattg ggaaaagaga ttccgaaggg
    32641 atggaaaaac gtgagtttat cgagcattat agatataaag gatggtaccc acgattcacc
    32701 gagtccgact gaaaatgggt ttccattagt cacttcaact catttgaatc cgtatgatat
    32761 ttcgcttaat gaaacttaca atataagtga aagagattat attcaaacta ataaaagaag
    32821 taaggttgaa aaatttgata ttttgtttag tatgattggt actgtgggtt tggtaaatta
    32881 cgttctttat gatgatataa attttgcaat taaaaatgtt gggctattta aaacttctga
    32941 aaagaaggaa tttgctgaat atattttatt ctatttgaaa agtgattata tgaaattata
    33001 tattgcttca aatccaaacg gcagtactca aaactatgtt acacttgggt tcttaagaga
    33061 catgccaatt ttgttgccat taaaaaagat aatattacaa tttgaatcta tttcagagaa
    33121 gataatagaa actattcttt tactgacaaa agaaaatcag aaactaatgg agttgaagga
    33181 gttgttgttg tcgaggatgg cgaaattggg taaaatataa acaatatgaa aattaatttg
    33241 aagcaagctg ttagagcttt ttttcaaaat ccatctttag aaatggtttt cattgaagca
    33301 gtcgcaaatt caattgatgc caatgctaca caaataagca ttgatatttc aatgaaatct
    33361 ctttcacatc aagagtcatt agagattact attactgaca atggggaagg attcactgat
    33421 gaaagattta agaagtttgg agagctattg tctgttaatg agaacactca taaaggcgtt
    33481 ggtcgcttag tataccttgc atattttgat gagatattag ttgaaagtca atatggtaaa
    33541 accaagcgga cttttactta tgatgcttct tttgatgaag ataaaagcga catgaaaaca
    33601 aatccagaag tggatgaaaa atttgaaaca aaaattgtgt ttaaaaagtg ctcattgcaa
    33661 aggctcagta gttataatgt tgttcttcca gattatttga aacgtcgtct tttggaagaa
    33721 ttttacccga aattctattt gaaaaaagag gaaaaaaaca ggttggatgt ttctatagga
    33781 attcatgtct cagaaatttc aaaaaacatt tcaattggaa acaggaaaac cattataaag
    33841 gtaaccgaca tacctgaaat gaaaactgca aatgttgatg ctcgtcagat tcaacttttt
    33901 gaaaacatgg acttgaaata ttctattatt aaacgaaatg atttctctag tccttttatt
    33961 ataacagcac tttgtataga cgaccgaact tacaatatta aagatgtcat atctgacgaa
    34021 aatgtcccaa atggctacga gtgcgtattt ctcttaagct caacagtttt tgatgggcaa
    34081 gtggatgcat caagacagat tctacaatta aacgacacta ttaaacgagg tgttgtaaaa
    34141 ttattcagag ataaaatttc agaaattttc aaagacaaaa tacctgaaat tgctcaaaaa
    34201 aacgaaaaaa ccaaggaatc tttaagtaaa acatttcctc atttggttgg ttattttgag
    34261 gaggaggaaa ttggtgttat ttctagggat aaatcaattg aaattgctca aaaaaagtat
    34321 ttaagagatc aaaaagtgat cttggaatca gatagcttag acgatgatag gtttgataaa
    34381 acaatagaaa tatcttctcg agctttagct gaatacatta tctacagaga aaaaattatt
    34441 gaaaaaataa gaacaataac aacggaagat cctgaagctg aaatacataa tttaattatg
    34501 ccgaaaagag caatttatag aggagatgat gtgttgtcaa atgtttacag taacaatttg
    34561 tggcttttag acaataaata catgacttat tcggtcgcaa tgagtgaacg aacaatgaaa
    34621 gaaattgcgg aagagatttc tgatgaaatt caaagtggga ctgataataa taaacctgat
    34681 attgcaatta ttttctcaga taatcctcaa gtagaaaaca aaaaggttga tgttgtaatt
    34741 attgaattta aaaaaagagg aattaagctt gcaaaaacag aagaagtgat tagccagctg
    34801 agacaaagag caagaaaatt aatgcgatat tatcctgaga aaattcagcg tatttggttc
    34861 tatggtattg tagaatttac aaatgagttt aaattatcat tgaaagatga cgagtacact
    34921 ccgctgtttt caaaagacaa gttgtattat aaggaaaata aaattcattt gtctgaggat
    34981 tcagatgaat atcatttagt tgggacatat atattgtcaa tagatgcatt tattgaagat
    35041 gctaaggctc aaaatgaaac gtttttaaag attcttaaaa gtggtttttt gaaatcaaaa
    35101 cactaatttg ccatgaataa taatcagccc aaactcatca aaaatcaacc gatcggcgaa
    35161 gacaagtttg aaggccaggc tcaggaaaat atcgccaaag ccgtagtaaa aatgcttgaa
    35221 gaaaacagtt gccagatgat tggcattgat ggtggatggg gaacaggtaa aagcaatctt
    35281 gttggcatcg ttgaaaagga attaaataaa tcaagtaaag gaaatttcca tgtcttcact
    35341 tacgatgcat gggggcatca aggtgatttg cacagaaaag ctattttgca ggaactgata
    35401 gagttcctat gtaaaacaaa aaaagttctt aaatgggatg aatggaaaga aaagctgaaa
    35461 actttacttg gaacttctgt tgaaacaaga cacaaaacta ctcctaaact gagtttggga
    35521 attatttttt cattcctgat ttttttatct attccgattt ttaatagtat cgcaaatgat
    35581 aaagatacag aaacagcaac aaaaatctgg atacaattaa ttcctgttgg agctttaatc
    35641 ttattatact tagggtattt aataaaagaa ttattgcgct ggataagtgg aaaatcaaaa
    35701 ctccggagca ttttttctat tgctgcatca gaaatgatat acgtgtataa gggaaaagaa
    35761 cttgaaacta cagcaactga atacaagcat gagtcaaatc cttctgtttt tgacttccga
    35821 tcatttatgg aggatatttc aacagcaata ggaaacaagc gattgatatt tgttgttgat
    35881 aattttgaca ggctacctat taatcaggtt aaagagttgt gggcgtctat tcatacactc
    35941 tttgctgaaa aaaacaaatg ctcgaacata agtgttatag taccatttga tagagctcat
    36001 attaaatctg cattcagaga tgaagataca cctgcaactt ctgatgaggc taattgctat
    36061 ggcaatgatt tcataaataa aacatttgat gtcatttttc gagtttctgt tccaatactt
    36121 tccgattggg aacattattt caaggctaag tgggaagatg ctttcggaaa gatttcctct
    36181 gaaaatgaaa cggaatataa tgcagtagtt cagatatatg atttattaac acaaaaaatc
    36241 acaccgcgag aaataattgc atttattaac gaatgtgttt ccactcggat cactcagaga
    36301 gaagaaatcc cattcagata tattgctctt ttcatcaaag gaaaagataa aatattgaag
    36361 aacccaattg aagaaataac aaccccaact tatcttgggg ctgttgactt tctatacaat
    36421 tatgatgaaa acttgccaaa atacatttcg gcattggttt atcaggttga ttcatcaaaa
    36481 gcgcttgatg ttgcgtataa gaaagttttg aaagatgcac ttgaagggaa tgaccagaaa
    36541 gttgttgtta agatttcagc atcaaaaagc ttttactcaa tcttgaaaaa cacaattaat
    36601 gaagtcacaa atattgaaaa cttaattctg gcattggaaa acatacccca agagtctttt
    36661 aaaagcccat caaattctga aactatttgg acatgtattt ttaaaagggc ggaaataaaa
    36721 actgacggaa ggccaggctt gcaaaactat cagcgtgcat tgttgactaa aatatctatt
    36781 gctgaaaaag agcgatatac acgaaaatta cttgctggtt ttacatcaag tagcgagttt
    36841 aatgcagtta cttatgcaaa taacattgag acaattgatg caattttgag acccgtcaac
    36901 attgatgttt attcgtattt gtcagataaa ctaacaggct tgaatgactt tgtttctctc
    36961 ctgaattcgc aatatcaaaa acctgctcaa tacagaatta aatgcaaccc tgatgaaatt
    37021 gatgtaactc ttgaagggaa gacaatagag cagttgaagg atatttcata tgttccaaaa
    37081 attatagaag agtacagtct tgtaaaatac aaagcaaagc ttatacaatg gattcctact
    37141 gcattgctgc acatttctca attggaaata ttatttgatc gaattaagga agtacaagga
    37201 tcaataccag ctacaataat tccagatgcg tatattaaaa gccatttcat ttcaacagga
    37261 gaagattcta atatttaccc agacctgatt tcaatgcgta ttgccagaca gaatgaattt
    37321 cctgccccat tggcaaatga ctttgaggtg gttttaaatt ccgcgaatga aaacctcata
    37381 gatagagtca cacgatgcat tcatttttat atttcaattg acaaacttct tttagggctg
    37441 ggagattttg gacagtatcc gttatataga ggaattgttc aaagattgat aatgagtcca
    37501 tccaataaaa aaattgaaaa cattgaacct ttattgtcaa attttgaaat gatttgttca
    37561 aatggagaaa tttctcctga acaattaatg aatttcctga attcgaatta cacgacagag
    37621 tttactccga caaatgtgcc tgttaatatt ccagtttatt tgattacaag tatttcccaa
    37681 aacgagagta ctctatcaga gcgttgtaga aatttagttc tggaatacct ttctacaatg
    37741 aatgaagaaa aatgggctgt ggctttttct aatatcaact cgtatgaagt tcatgcgtct
    37801 ttactgctca aaataaattg gaatacggtc gctcatattg catttaaaaa gacacttaag
    37861 aaaattgctg atggaggaat agctatttcg aataaagaac gttgggatga gcttgttcaa
    37921 tcttttgttg atcaaagaac catggagtca gcgtttaatg atgttacaga tacgctttgc
    37981 aacagctcaa aagatttctc aaacgatatg tttgtatttc ttgcgccatg gctatttgag
    38041 cattctacat ctcttttaag caaaaaagag gttcttagaa aaattttccc tccaacaata
    38101 ttaaccgatg agcgctgcct tcacattctt aattccaata gagagaaaat gattcaggta
    38161 cttgcatcgg caggcgctga atcatatgat tttaaagatg cattgattga tcatgcaaga
    38221 ggagaaaatc catcattcag gcaattagct gaatctttaa aaatagacat tcctgcagaa
    38281 ccggaagaaa gcgaagagga taccaacaat gaagctgata acaatgagac tatagacaag
    38341 taacaaaatg catctttggc agaaataaac aaaggatatt cttatgcacc aaatcaaaca
    38401 actttacgca atcactcaac agctgaggga aaagtatgca aagtggaata aacagttcac
    38461 gctggatggc aagctggtgg gcgatatggg ggaggtgctg gctgctgaga agtatgggct
    38521 ggagctgtat gacgagaact atgaaatata tgacggatgg gagacagcca ccggcagaaa
    38581 ggtgcagata aaggcttcgt ttaaaggtta ttttcaattt ccgtatggcg aaaataaaat
    38641 tccggaatat tatctggctg taataattga cgagcatggg gtactgcatg aggtgtataa
    38701 tggccccggg cagtttgtgt atgataatta cgtagtgaag aataatctga agccatataa
    38761 gaattcatac tatactgttt cgaaaggaaa attgtcatcc ctgaatgatg tagttccaaa
    38821 ggaagacaag attatcgaag ttacaactgc aaaggaaaac taaccatgga ttttcaacag
    38881 cttaaagtat ttttgcagac ccatgagatt ccggtttcag aagaaactat tgggtttctc
    38941 gaaatcattg gaaagcagca ccacgagaac attaacagca ccatatatgc gcatttcata
    39001 aattgcggtc atgatgtcgt ccggaacctt tttgttgagt ctttgtgcga aattgtttct
    39061 gaaagaagtg gaaaaagcct aaatctgaaa cttgcactcg cacgaactga agttaacact
    39121 ggcagtgggc gcattgatat attaatcagc gatcagggta accaaaacgc aattatcatt
    39181 gaaaacaaaa tctaccattt tcttggcaat gagttgattg attattggca gcatgtaaag
    39241 caacctgatg ataaaaaagt aggagtattg ctgacgctat atccccatga aatacccgaa
    39301 aatgtttccg ggaagttcat taatgtcacg catattgaat ggatcaaaat ggtcaaaaac
    39361 cgattaaaac cggaaattct gccatggaaa tatgctgtat atattagcga ctttatttct
    39421 actattgaaa atctgacagg agattatccc atgaatgaat ctgttaagtt ctattttcag
    39481 catgccggcc agatactggc agcgaatcaa gccatgcgtc aaacgcatga tttcatcaat
    39541 aatcagtttg aaataatagc aaaaagaatt ggttggtcaa cctatggtgg ttcaatggat
    39601 tggagaaatt tctgggatga gcggaataag cttgacacct atttgacaat tattgttgaa
    39661 gacttgctaa agggtaaatt gtgtttcact cttattctgg aattgggtga aagagatatt
    39721 gtttactctg ataaattgag aaatgagcta aaggcctgtt tgaaaaccgg tatgtattgg
    39781 ggaaaacatg aagtaaacta tatacattta ctttgtaaga attatgaaat agaattggaa
    39841 gatctggaac attttgccga ttttgtagaa agaaaaatcc gcgaagattt tgctgagctt
    39901 acgcataagg caataaaatg tttgtatccc gaaactgaca ttagtcattg ggagaatcat
    39961 tttattggtg cgtgatagga aaaataaccg caatgaactt tctcaaaaga aaacatcaaa
    40021 atcgtcggcc tcaaagagca tttgcgggtg gactgatatt atttcgacgg ggatatgtca
    40081 attgttcgct cccataatct gatattcaaa tttagtatat agtagtgtaa tggttttaac
    40141 aacagacaaa gatatagctg gtaatgatat tgaaccccat gataaaagaa aaagaaacga
    40201 tatggttggt ggtaatgaaa ataccagtat atttgtccag taaattaacg aaaaaactgg
    40261 acatgttcct gatgataatc acaatgacaa tactatgaat gttgctgatt atattagtgg
    40321 taagatagaa cgactgccta aaggctatgt tttcacctat gctgattttg ataccaaggt
    40381 gaatcaaaag gaagcggtta taaaagcatt gaaccggatg gtgcaatcgg gcaaaattgt
    40441 caagctgtcg aaagggcgct attataaacc cgagaccacg ccattcggca atctgctgcc
    40501 cgatcagaaa caagtggtga aagacctgct ggaagaaaac ggcaagccga cgggctatct
    40561 gaccggttac agcatataca accaattggg gttaactact caggtgagca acaccataca
    40621 gattggaaaa aatcaggttc gccccggctt caaaagagaa cgctacacaa ttgcatttgt
    40681 aaagcagaaa aacaccatta ccaaagagaa tattcccttg cttcaattgc tggatgccat
    40741 ccgctacatc aaaaaaatac ccgatgccag tacagaagct tcctgcaagc ggtttctatc
    40801 tatcatcagg aagttttccg acaaagaaat cagcaccctg ggtcgcctgg cattaaaata
    40861 tccgcctgca acgagggctt tattaggagc attgcttgat caattaaaaa aaggtgctgt
    40921 aacagagccg cttttcaaga cattaaatcc gattacaaaa tacaaactgg caggtgctgt
    40981 aaaagcatta tccactgctg aaaaatggaa tattatatga gattgcatga agacaagata
    41041 ttgttcaaac aggccgttcg ttatgcgtcc gatcaaatgc agattcctgc tgtttatatt
    41101 gagaaagatt attgggttac ctatgcattg tctgctattt ttaaaaacga agccggaaaa
    41161 gatgttgtct tcaaaggggg aacagcatta tcaaaatgct ttaagatgat tgaccggttt
    41221 tcggaagaca ttgatctggt tgtactgagg cgagatggag aatcagatgc cagaatgaaa
    41281 acaaaactta aaaacgtcag caacgcaatt gaaccggtat taccggaaat agagattgac
    41341 gacatcaccc gaaaaagagg aatgaaccgc aaaacagcgc atcgttacag caaggaattt
    41401 gatggaaatt ttggtcaggt gagagatgta attattctgg aaacaacctg gctgggatac
    41461 tacgaaccat ttacctccag cagtattgtt tcatttgttg gtcaaatgat gctggataat
    41521 aatcaaactg atatcgctga aaaatatgct ttactcccat ttgacttgtt ggtattggag
    41581 ccaacaagaa ctctctgcga gaaaatcatg agcctggttc gtttttccta tggcaaaaac
    41641 ccgatcgaag atttaaagaa aaaaataaga cacacgtatg atcttcatca gttgttgaag
    41701 cataaagaat ttgcagaatt cttcaaatcg gttgcgttcg aaaaaatgct cttaaaggtt
    41761 gcggatgatg atgttaggag ttttaagaat aataatgaat ggttgaaatt tcaccccaat
    41821 gatgcaatgt ttttcgcaga gcttgtacag gtgtgggaca agttgaaaac ggtttacagt
    41881 ggtgacttta aaaaactggt ctatggcagt tttcccgatg aattcgaagt gtatcaaaca
    41941 ctaaaacaga tcaaagaaag aatgagcaca attcaatggt cgataaacat ccaatcataa
    42001 aaatgaaatt caccgaagcc agtctggaac aatcatttgt tgagctcttg ctgagcgagg
    42061 gctacgcaca tgtgcacggg gacatgattt cgaaagcacc cggcgaagtg ctgattgaag
    42121 aagacctgat gcgttttttg ctttcggagt acgcgaagga tggacttacc gaaactgagg
    42181 ccagatcaat tgttttgcag ttgcagcggc ttccggcaag cgatctgtac aacagcaact
    42241 gtacttttat gcggatgctt tcggatggat ttattctgaa aagagaagac cgaaatcaga
    42301 aagacattta tattcagctc gtcaattacg cagggcttaa cagacaccgg cagcctgatg
    42361 cagaaactct tatcaccatt gccgccgagc ccgaagccac atatccgccc gatacaaaca
    42421 tttacaaatt tgtcaatcag ctggaaatac atggcaacga aaaacgcata ccggatggca
    42481 ttgtgtacat caacggattg ccactggtgg tgtttgaatt taaaagtgcc atacgtgaag
    42541 aggcgaccat acacgatgca tacaaacaat tgacaattcg ttatcgcaga gatattccgg
    42601 agcttttcaa atacaacgca ttttgcgtca tcagcgatgg cgtgaataac aaggccgggt
    42661 ctttctttgc gccgtatgaa ttttattatg catggcgcag ggttgccgga tcagcaaaag
    42721 aagtggacgg tatagatggc atgtacactt tgattcaggg aatgtttcat aaggatcgtt
    42781 tgcgcgacat catccggaat tttgtttaca tgcctgattc gagcaaaaag gaagaaaaga
    42841 ttgtttgccg ttatccgcaa tattacgcag caaaggcgtt gtttgaaaat attcgcaaat
    42901 cgcatagacc tcagggagat ggaaaaggcg gaacttattt tggcgctacc gggtgcggta
    42961 agagtttcac catgctgtat cttgcacggt tgctgatgaa aagcgaatat ttcgaaagtc
    43021 cgaccattgt gatgattacc gatcgtaccg atctggatga tcagctttcg ggacaattta
    43081 ctaacgcaaa aaaattcatt ggagacaata cgattatcag tgttgagagc agagcccatc
    43141 tacgcgaatt attgcaggac agacaaagcg gcggcgtgtt tttaaccact attcacaagt
    43201 ttaccgaaga caccgaatta ctgaccgaca gaacaaatgt catttgtatt tcggacgaag
    43261 cgcaccgcag tcaggtgaat cttgatcaga aaattaaagt cacagacaaa ggtgtcaaaa
    43321 agacatttgg ttttgcacgg tatttacacg attcattgcc cggcgccacc tttgttggtt
    43381 ttaccggaac gcccattgat gctacgcttg acgtgtttgg caaagtggta gatgcataca
    43441 ccatgaccga atcggtgaaa gatgaaatca ctgtgagaat tgtgtatgaa ggccgggccg
    43501 ccaaagtagc attgaaaaat ggtgagctgg aaaagattga gaagtattat gaagaagccg
    43561 ctgctgcagg tgcaaacgaa tatcagattg aacaaagcaa ggaagaaacg gctaatatgt
    43621 acgccattct tggcgatcca aaacgactga aagctgttgc ggaagatttt gtaaaccatt
    43681 actgcaacag ggtttcagag ggcgcaaccg tcaggggcaa agccatgttt gtttgcagca
    43741 accgcgagat tgcttatgaa ttgtataaaa acatcatcga aataaaaccg gaatggaatg
    43801 aggtacgcgc agctgatcct gcctgtgccg gcacggcaga cagggaaggc gcagagctca
    43861 ccgacaagga ccgaagagaa cttaagccgg ttgagcagat caaaatggtc atgacccggg
    43921 gcaaagatga tccggacgag ctgttcgaat tgttaggcac aaaagaaagt cgcaaagaac
    43981 tggaccggca attcaaaaac gaaaaatcga atttcaaaat tgcgattgtt gttgatatgt
    44041 ggcttactgg ctttgatgta ccgtttctgg ataccattta tattgacaaa cccattcagc
    44101 agcacaatct gattcagacc atttcgcgtg tgaaccgcag gtatgaaggg aagaacaaag
    44161 gtttggtagt ggattacatc ggaattaaaa aacagatgaa tcttgctctt gcaaaataca
    44221 ataaaggtga tgagaaaaat ttcgaagaca ttactgaatc actgattgtt gtcagaaatc
    44281 atctggatct tttagacaag ctgtttcata catttgacaa atcaaaatat ttcaaaggca
    44341 caaccattca gcagttgaac acattgaatc tggcagctga atttgttcag caaaccaagg
    44401 actttgaaac acgctttatg ggttttgtaa agcgacttaa agctgcttac gatatttgtg
    44461 caggaagtga tcaactgact caaccggaaa gagattacac gcatttttat atggcagtcc
    44521 gttcaattgt gttcaagctc acaaagggca atgcgcctga tacagcgcag atgaacgcac
    44581 gtgtgcggga aatgatcaaa gatgcattac agagcgatgg cgttgaagaa attttcagaa
    44641 ttggcgacaa aaacgaaaac gggcaggatc tttttgatga agactatctg gcaaagattg
    44701 ataaaataaa attgccgaat acaaaaatca aattgcttca acaactgtta gccagagtaa
    44761 tcggagaaat caaaaaagta aataaggtaa aaggcgttga ttttaccaaa aaaatgcaag
    44821 ccattgtaga gcgctacaac aaccgcgatg tcaatgatat cctgcgaagc gaagtgtatg
    44881 aagaaatggc tgaagccttg acaaatctta tctgggatgt tcaaaaagaa tttagagccg
    44941 gtgacgagtt aggaatcgac tttgaagaga aagcgtttta cgatattctg aaagagcttt
    45001 gtatcaagta tgatttcatt tatcctgaag ataaaatgat tgaattggca aaagcagtga
    45061 aggaattggt ggatgggcaa gcaaagtttc ctgattggaa taagcgggat gatataaaat
    45121 cagctttaaa agtcggtctg attcttttac ttgacgaatt tggatatccg ccagtagagc
    45181 gcgacgaagt gtatgttgag atttttgagc aggctgaaaa tttcaagaaa aatcagaatt
    45241 aatgagtgag cgacttaagc gcattaacga tgccatcatc atccatcaaa tcacacaact
    45301 ccccattttt ccttattttt gttcaggata ataaaaatga ttctgcaccc atgaaaaagc
    45361 cctcccaaac taatacgcat ggcaaggcca agaaaacttc agatgaaacc aggaataact
    45421 aaatggacaa aataaaattc agatatcatc ttgacgaagc tacaaagcag ttaattgtca
    45481 tgaccaagac actttgttac aatgactttt ctgacaatta caattatatt ctcacaccaa
    45541 acttacggac agttgataaa gacgataaac atttaaacga aaaagaaatt tccgtattaa
    45601 attcctggaa caaatttgaa ggaaaacttt tgacagccac tgaagttgtt gacttatttc
    45661 atcatgacaa caaagttccc gtatggatag atatgacagt ttatgaggct agacctgact
    45721 tgactgttat tgatttattc tgcagcagac gacttcggga agagaaagag ttaatgcatc
    45781 agggactcca tccatttcat gtacaagttg cgacaccacc tgacagtttg aaggtagaaa
    45841 ttgacggaaa gtttgatgta aattggaaaa agaagcgtga cgaccacaat aaaaagggag
    45901 gcttttgaaa agactgaaac aaatatttgc ttagccatgg tacgattgcc aactttcaac
    45961 atgggtttca tagcagacgg ggtgatgtgt aaacttggcg ctgtttgctc cgtaatgtgt
    46021 gtcgctgaat cagcgggata ttcacattta atttcaaaaa aggctttatt atgagaacat
    46081 ttttattcat tacatgcgca cttatcatta atctcacctc tgggcagacg gcaaaggact
    46141 attttaatga ggaagtcaaa gatgctcagt tggcaaccgc tcaaacaata atgacagcgc
    46201 ttgaaaatgg gaaaattgaa ttagcaggga tgttttttga cgattcaatt aagaatttac
    46261 gaagcgaact caactatatt tcatcagaaa tatccaaggt aaaagctgaa accaagctct
    46321 caatagtaat tgttttcgat caggcatcaa acatttacag gtgtcgttat tctaatgaca
    46381 gcgatgaact gtttcaggta gatctgttta tgagcgaagg tcaggcaaac tcaaaagtca
    46441 aaaaacttga aatcaaaaat tcagatgttt taaaaatgga ggcgaaggaa cggatggaaa
    46501 tgaaagaacc tccgctacca ccgaattgaa atatacgaaa aatcaacttt ttattttcgt
    46561 aaatttgcac cgtaactgat tttccacacc catgaaaaaa ctctctcata ccgataccaa
    46621 aggcaaagcc cgcatggtgg atgttggcga aaagcccatc cagagccgga ctgcaatagc
    46681 ttcgggaaaa ataattttgc aacccgcaac cattaagctg attaaggaaa acggcatgaa
    46741 gaaaggcgaa gtgctgagca ttgctgagtt tgcgggcatc atgggtgcca aaaaaacatc
    46801 ggagctgatt ccgttgtgtc atccgctgtt gctcacggat gtgcaggtgt cagcaaagct
    46861 gataaaaaat ggtgttgaga tttctgccac cgtgaaatgt cagggacaga ccggcgttga
    46921 aatggaagcg ctgaccgcgg tgagtgtggg tttgcttacc atttacgaca tgtgtaaagc
    46981 cgttgataaa aaaatgatat tgctggatat tgaactgctg agcaaaacaa aaatctgaat
    47041 tcaataattt tgtggccatg aaaattgtca aaagaatttt tctgggtttg ctgattctga
    47101 ttgtcattgc aaatatttta attcttgtat ccggcaattc gtatatctat aaaacgctca
    47161 tttatcaaca tgtgggcatt gaggattttg aaattttcga aaacaggatt gttgaagccg
    47221 gcactccaac ggagattcca aacggcacat ataaaaccga gctgccggat tctttcagaa
    47281 agttcatggt tgaaacacaa acaaccgcgt ttctcgtttt gcaaaacgat tcgatcattt
    47341 acgaagaata ctgggatggc tatggccccg acacggtttc gggatctttc tctgttgcca
    47401 aaaccattgt cagcatcatg gttggaattg ccattgatga aggaaaaatc agcagtgtga
    47461 aacaaaaagt ttcagatttt ttgccagaat tttcggaagg tcttaagaca gaattaacca
    47521 tagagcacct gctcacaatg actgccggct ttgactggga cgaagcttac gccagtttgt
    47581 tttcaccgac tacaaaagcg tattacggca acaatctccg gaaaatggtg gataaactcg
    47641 acatgaaagt aaagcccggc acattgcatg aatatcaaag catcaatcag gtggtgctgg
    47701 ccatgatact tgaaaaagcc accgggaaaa cactgagtga atacacatcg gagaagctct
    47761 ggacgccgct tggagccacg cacgacgctt tgtggagtct cgaccgcgaa gatggtcttg
    47821 aaaaagcgta ttgttgcttc aattccaatg cccgcgattt ttcacgcatt ggaagtcttt
    47881 atctgcataa aggtgaattg aacggaaagc gcattgtttc ggaagcatat gtattgaact
    47941 cggtaaatcc ttgcatgatt ccagatgaaa ccggctctcc ctgcgattat tacggatatt
    48001 cgtggtggct ttcaacaata gataatcacg catttttcta tgcccgtgga attctcgggc
    48061 aatacatcat tgttgttccc gaatacaatc ttgtaatagt tcggctgggt cgtataaggc
    48121 cagatcatgg cgatattcag gcagccgatt acatagtttc aaacatttta aaaacgctga
    48181 aaacaaatga atagaattga aataaaatcc gttaatgttt cggataaaaa aggcattgta
    48241 aaagttccgg tagaagccat tgaattgtgc gagaccggca tcataggcga cgctcatgca
    48301 ggggcgtggc atcggatggt gagtatgctg gccttcgaaa gtgtggagaa atttgagcat
    48361 aaggcaaaac ggaaaattgc tttcggcgaa tttgccgaga acattaccac gcaaggattg
    48421 ttgctgtacg aaacgcatcc gcttgatcgc ttcgaaggca acggcattga gctcgaagta
    48481 acacaaatcg gcaaggaatg ccatggtgac agttgtgcga ttttccgcga agtagggaat
    48541 tgtgtcatgc cgaaggaagg catttttgtg cgtgtgctga aaggaggcaa gatgaaaccc
    48601 ggcgacacca tggaatataa acccaaagtg cataaaattc atgtcattac cttgagcgac
    48661 cgcgcttttg ccggtgaata cgtagatctg agcggacctg ccattgaaag caaactgaaa
    48721 agtttttttg aatccgaaaa gcgtgtttgt gaaattcagc gcaccgtgat tgccgatgat
    48781 tctgaagcgt tggagacatt gattgttcac agcgtgaacg ccggattcga tgcgattttc
    48841 acaacaggcg gcaccggcat tggcccacgt gatattacgc cggaaaccgt ttggccttta
    48901 ctcgacaaag acattcccgg tattatggaa aacatccgac tgaagtcggc acaaaaaaat
    48961 ccgaatgcat tgctgagccg cagcattgcc ggcgtgatcg gaaacacgca gatatacacg
    49021 cttccgggca gtgtaaaagc atgcaacgaa tatgtcgaag aaattctgaa gacctacatt
    49081 catctgctgc tgatgctttg caaggtcgat gctcattagg acaagctatg cgaagtttaa
    49141 acttcgcata gcaacagtcg cagcacctca ttcgcctgca aagcagcagc aatggtcact
    49201 ttcggcgcca ttggcggatt tttttcggaa acgctttcga ggaaatcgcc caccactatc
    49261 aaattgccgt tgcgtacaac ccgcatctga tcagcatcat cgaggcctcc cagaccagaa
    49321 gcacacacca gtggtttttc cggaaaaata tccaatactg tttcgatgag catttgtttg
    49381 ttttcggcca ggtcaaaagc ttcgacaatc acatcgcaag gcgcaaataa cagctcaatt
    49441 ttttcgcggt caagcctgat cacgtgcgat tcaatctgag catgcggatc aatcatcagc
    49501 agattttttt tcagggtatc agccttgggt ttgtcaatct gatcataaaa aaagaactgt
    49561 cggttgaggt tggatttctg aataacatcg aaatctgcaa taataagttt tgttacaccg
    49621 ctgcgaacca acatggcggc gcaattcgag ccaagcccgc cgcagccggc aataccaacc
    49681 gtgtactgtg aaactttttt cttgatttct tcaaatgtca ttgtcaattc ctttcatgca
    49741 aagatagtaa aaacggatgg taaaacgaag tcaatatgca aaacaatgca tattgaaaat
    49801 aagaaatact gcccgtttcg aatgtttttt ttgttatttt tgcaaaacaa aaatacagaa
    49861 atatggccat caatattgaa gacctcaaaa ccaaccgcaa gattctggca tcgtttgatt
    49921 atcagattgc ccctgtaaat cgtggttata cagacaaaac cttatacatc aatgttggtt
    49981 caggagaaat aaaaagcaag gatgttcctg cccaggtgaa agaaaaattc atcggtggaa
    50041 aaggttatgg actcagactg ctctgggacg ctgttacacc aaataccaaa tggaatgatc
    50101 ctgagaatga aatcaacata tcaggcggtc ctatctgtgg aatcactcaa tacagcggcg
    50161 ccgggaagtc gattgttgta actctttctc cgcagacaca tattcccgtt gacagcaatg
    50221 tgggtggatt tttcggacct tttctgaaaa ttgccggttt cgatgctctt gaattgcagg
    50281 gcaaagccga aaaagatgtt gttgtttata ttgacgcggc caaaggtcgt gttgaaattt
    50341 ttgaagatcc tggctttgag cacgattcac acatgttagc tgaagagctc acagatcttt
    50401 ttgccaacga cgaaaaagag aaaaagaata ttggtattgt ctcagctggt gatgctgccg
    50461 atcactccta tattggaatg ctgaacttta ctttctacga caacaaacgt aagaaaatcc
    50521 gtttaaaaca agctggccgc ggtggtatcg gcactgtttt ccgtgataaa aaaatcaggg
    50581 ccgttgttgc caaggttccc ggtgtgaccg gcaatctgaa taatgtggtt gaccttgata
    50641 ccattatgga acgtggccgt cgttttaaca aggaaatgcg tgaactcgac gacagtcagt
    50701 gcgaaatgcg taaaaaaggt acggctcacc ttaccaatat catgaatgat tatgatttgt
    50761 tgccggtgca taatttcaaa ttcggggtgc attccgatgc cggtaaaatt catggtgatg
    50821 tctggatgaa gtattttacc cagggtgtgc ctgatggctg ctggattggt tgtaacatga
    50881 gttgtgccaa gggcgttgat gattttgaac tgaagactgg cccttataaa ggtcagaaag
    50941 tgattgttga tggacctgaa tatgaaacaa cgtcatcgct tggttccatc atgggcattt
    51001 ttgatcctga atggactatt gaagccaatt tttattgtga tacttatggt atttgcacca
    51061 tttcatgggg aaccattatg ggttttgtga tggaatgtta cgaaaatggc attcttaaca
    51121 aggaacgtac aggaggtctc gatctgagtt tcggcagtaa agatgatgcc atggaattgc
    51181 tgcatcaggt tgcacgcggc gaaggcttcg gaccaaccgc cggcaaaggc attcgttaca
    51241 tgaaagatct tttcattgcc aatggctggg gtgccccgca gtttatcgaa gatatcggca
    51301 tggaaaataa aggtctggaa tacagtcagt acatttccaa ggaatcattg gcgcagcagg
    51361 gcggttatgc catgaccaac aaaggtcctc agcacgacga agcatggttg atttttatgg
    51421 atatggtgaa caaccagatt ccgacattcg aaaagaaagc cgaagcattg ttctatttcc
    51481 cgatgttccg cacatggttt ggtctgcagg gactttgcaa actgccgtgg aacgacgttg
    51541 agccagccga taatgctcag acagcggagc ccgccaaagt tccggaacat gttgataact
    51601 acgtggccat ttataaagcg gttaccggaa acgatttcag caaggaagaa atgattcggc
    51661 agagtgaaag agtgtataat ttccagcgcg tattcaatat ccgcatggga aaaggtcttc
    51721 gtaagaatga tgcacagccg taccgcgcct gtggtcctgt tacaaaggaa gaatatgaaa
    51781 gccgcgtaga acgctacgat aagcagatga aagaactcat tggcattgac ccggaaggaa
    51841 aaaccaccga ggaaaaaatg gccataacgc gcaagtaccg cgaagatcgt tatgaacaac
    51901 ttctcgatgc tgtttattca cgtcgtggct ggactaaaaa tggcgtgccg acaattgaac
    51961 acatgaaaga attagggatg gatcttcccg aagttatcga agttattaaa ccgcttcaat
    52021 aacgaattca tttttacaga caataaaaaa agccctgaag aaattcgggg ctttggtttt
    52081 ttctgtaact tctggactta tccaaccaac atggctcctg caattgcggc ggccatgaca
    52141 atgatgtaaa tggtccagag tgcggcttct tttactgtgt tcatggcttt aatattttta
    52201 aggttcgaca tttttttgat tttgttactg taaaggtagt tcttgtactg tctggaatca
    52261 atatagtctc agtttacggc tgaaacgggc aagttaacgt atgcagtttt ctgcatttat
    52321 gcagttttcc ttttctgtta ttcataaaac acgggttttg tggtctggtt gcctgactaa
    52381 acccttcgac gaattgattg tatttccatc agacgatttt aaagaattct ttttcgagga
    52441 ctttattaat tattttggtt ctgcaagttt ttatgtgaaa catgaagctt gaggattaat
    52501 caaccaacga t
//
LOCUS       DAOPKE010000010        52454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_653_length_52454_cov_12.902326, whole genome shotgun sequence.
ACCESSION   DAOPKE010000010 DAOPKE010000000
VERSION     DAOPKE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_52454_cov_12.902326"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PKY63_02540"
     CDS             complement(<1..1399)
                     /locus_tag="PKY63_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE99523.1"
                     /translation="MKKQLKHTISHHDPVHDSAIKHVQGTTQYIDDMPALQGELQAWL
                     VTSPVAKGKIKSIETGNALSLPGVRCIITAKDIPGDNQMGPVFHDEPCLADGQVQCIG
                     QAIALIAADTFEIAHQAALLVKLNIETETPILSIDEAIQNNARLQPERRIERGDPDSI
                     LHSCEHVLHGQFRSGGQEHWYLETQICLAVPGENDEMTCYSSTQHPSETQAVIAEVLG
                     IGRNQVVVETRRLGGAFGGKETQGNHLAAWASLLAAKTRKPVRLRLTRDMDQSITGKR
                     HRFQSDWKIGFDNDGLIEAYTIIFNADAGCASDLTMAILERAMLHAENAYYIPNIRII
                     GNSWKTNLPSNNAFRGFGQPQGLAVIENVIDQMARYLRKDPNQIRQLNYYGIHERNIA
                     PYGASVKENKLSLIHEKILNSSDFDMRKKITDEFNAKNQIIKRGIAMVPVKFGISFTT
                     AFLNQAGALVHIYADG"
     gene            complement(1402..2856)
                     /gene="xdhA"
                     /locus_tag="PKY63_02545"
     CDS             complement(1402..2856)
                     /gene="xdhA"
                     /locus_tag="PKY63_02545"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HPE99524.1"
                     /translation="MKTKQHISFLLNGELIRIDFAADNIKPSTTVLNWLRKDMHHTEV
                     KEGCAEGDCGACTVVIATPDATGKALEYKAVNSCLLFLPAIDGKLLITSRGLSTETIY
                     SDELHPVQQAVVHEHATQCGFCTPGFVMSLFAQYHNPAPADDNEINNALAGNLCRCTG
                     YDSIRKAAKAVMQNRHPDAFDKEEKILIEKLNSITPYSQFEIQIQQQLYIRPISLEQT
                     LTVRKNHPEAIVVNGSSDIALQQSKHFKHIPAILDISGINELKTFEITENKLKIGAGL
                     SLENIRIATKNVFPAFNDILSVFASRQIRQVATLGGNIGSASPIGDTTPLLMACNADL
                     KLVSEINSRIIPLRNFIVSYRKTILEADELIAEIHLPLPKENEIVKMYKVSKRNDMDI
                     STVSAAFNLTLDNGVVTAVSIYYGGMAAMTIPATEAESFLTGKKWNASTIHEAMNIVK
                     KSFTPLSDARSGAEFRNIAAANLLLQFYNDTLIS"
     gene            complement(2853..3617)
                     /locus_tag="PKY63_02550"
     CDS             complement(2853..3617)
                     /locus_tag="PKY63_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPE99525.1"
                     /translation="MNFFNDISETINNNIAAVLCIIIGAEGSTPRKPGSKMLVFADGS
                     IKGTIGGGSIEKEAIELARNMIGTDAPSVQSFNLANDLEMHCGGRMTVYFEPLLQQPE
                     LIIFGAGHVGKALAKLASISGFKISVADNREGIYATWPQNLATTYEMPFEEAAEKIKF
                     HSNSYIVVTTYKHSFDSEVVAMLIDKPHKYIGMIGSVRKVDTARKRFSEEFSMPSNKI
                     NSIDMPIGIDIACETPEEIAVSILAKLIDVKNKNNR"
     assembly_gap    3741..3840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4035..5441
                     /locus_tag="PKY63_02555"
     CDS             4035..5441
                     /locus_tag="PKY63_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE99526.1"
                     /translation="MFKEFLLHKVFGIIHQRAVEKSLRCYVVGGYVRDILLERPSWDV
                     DIVVEGDGIELAEYVASQLKPHPKVSVFKNFGTAHFVYDETEWEFVGARSESYQRGSR
                     NPEVIPATIEEDMLRRDFTINAMAVSLGADFADLLDLFHGQNDLKKGVIRTPRDPQIT
                     FSDDPLRMFRAVRFASRFDFEMAPEVFQSIRSNIHRIGIIAPERISEELNKMLLHKNP
                     SVAFYQLEKCGLLQSVLPELTALKGVENMHGILHKDNFTHTLGVVDNVAAVSESLWLR
                     WAALLHDIGKAPTKKFDKVRGWTFHGHDAVGQRMVLKVFKRLHLPQNEKMHYVAKIVG
                     LHLRPISLVEEGVTDSALRRLIFEAGDDLEDLLIHAEADITSANKQKVKLYLSNFAEL
                     REKLAETEEKDRIRNFQPPVSGKEIMEVFGIGPSREVGIIKNAIKDAILDGDIHNDRS
                     EAWQLMLKQGEKLGLIPR"
     gene            5506..6075
                     /locus_tag="PKY63_02560"
     CDS             5506..6075
                     /locus_tag="PKY63_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99527.1"
                     /translation="MHAYKFRVTSDENDEFLREIEVLANQTFEDFHKVLVRTCKLAGN
                     ELASFYLCDNQWRKRREITLMDMNMESDEDEEKKSEANLIMKDVKLNQIIDDPHQKLI
                     YVYDYLNMYMVNIELIRIGQADLTLHFPIVSKEVSDIIAPPSKINRLVEDEEVVFDED
                     ELHGSEEEGDADDENLTEEQFYSDDSSDI"
     gene            6099..7508
                     /locus_tag="PKY63_02565"
     CDS             6099..7508
                     /locus_tag="PKY63_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99528.1"
                     /translation="MDSSDSFFSEEEIESLLMRFRNAVAQDNTEYFEPDELEAIADAF
                     LKRDDLALAKAAIDYGFEMYPDNEDFQILNIHYHVLDNRSDSAEMLARNFIDKYPENA
                     EVCLVLASILSDRGNHREALDMLEHARELDAESEHAITALSMEYQVLGQHRKAIELYR
                     ELLERNPDDEFALNELVFVYEVANEITEAIRFLTDFTDQYPFNQQAWFHLANTCNINN
                     EPMKAIDAYDFALAIDPSFMPAMFGKGHSYMFAGDHLKAIDCFKETAETDGFKAASYL
                     SLGEAYSQMNNYGLASDYYLKSIESDPSFNEARMGLAYCLYMMNLDEEALRHVDEAIL
                     NEPERSDAYQLKGTILMNLERYPEAAIEFRSAIRADKENDAAWLDLSETHHFMNGVSD
                     ALSILYEAEEVLESDNAMIVYRKAALLFEAGKDQEAVSQLLTGLSINAELYTSIYSYN
                     KELENNEIIQEIVTMFLNQ"
     gene            7513..8292
                     /locus_tag="PKY63_02570"
     CDS             7513..8292
                     /locus_tag="PKY63_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HPE99529.1"
                     /translation="MMNFEMSHLPERTVKPRTYGVNMVMDKGLSLREAENMMQASGHL
                     IDFVKLGFGTAVITGGLDEKIRFYHNAGIRLYFGGTLFEAFLIRNDLDGFKRVLDKYK
                     MDTLEISDGSMDLEHDLKCGYISEFAKNFTVLSEVGSKRTDVILTDEQWLQEMQKEHA
                     AGSSFVIAEAREAGNIGIYDSTGAAKKSLITLITEHFDINKILWEAPQKNQQLYFIKE
                     FGSNVNLGNIAPSDVIPLETIRLGLRGDTFFHFLPDMLKQH"
     gene            8329..9072
                     /gene="aroE"
                     /locus_tag="PKY63_02575"
     CDS             8329..9072
                     /gene="aroE"
                     /locus_tag="PKY63_02575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPE99530.1"
                     /translation="MKHYAIIGKPVSHSLSGQWFSKHFQKMAIDADFRMIEPSVDEMN
                     DFRNWVSGNQFDGLLVTIPFKMEVMKFLDETEKLAKTIGAVNVIHVSDKKLIGYNTDH
                     LAFEAEMKRLRPAGFKKAILMGIGGAAAAVSMALERMSIPVIKVSRQKKTSNILYTDL
                     TEKIVRDADLIINATPLGMGNLKNEFPPVNYDWIQKNALAYDLIYNPAETLFLKKCAE
                     VGCQTANGQGMVEFVYERALEIWGLTHKA"
     gene            9441..9686
                     /locus_tag="PKY63_02580"
     CDS             9441..9686
                     /locus_tag="PKY63_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99531.1"
                     /translation="MKKNHLLQLMYGVLILVVIITTAQSCNTFRTWFGTGGDFDRKHV
                     RAERKALREQMEEDDLWGGDDEYNPWEENDEYDRDRW"
     gene            9698..9904
                     /locus_tag="PKY63_02585"
     CDS             9698..9904
                     /locus_tag="PKY63_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99532.1"
                     /translation="MKTLRTIKLLLGATLLTLIGAVVQSCRPVINTCYKPAPPDDRDS
                     SEYKTIRNSCYDMPAEPMDTTMQQ"
     gene            9901..10179
                     /locus_tag="PKY63_02590"
     CDS             9901..10179
                     /locus_tag="PKY63_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99533.1"
                     /translation="MKKSKVILYRSLYFTGKIFSWQRLMLFAGASLLAASQACRPTQK
                     MCYEIAVPDSTTSSTDTLIMNDRDSLYQMIDCYEAPNYPDSVYKEPDN"
     gene            10201..10509
                     /locus_tag="PKY63_02595"
     CDS             10201..10509
                     /locus_tag="PKY63_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99534.1"
                     /translation="MKKIFLVSLHFIGRTVSWRWLSMIAGVALMTACSGKTDKAKVST
                     DNVKDSERVTCYEPVILQDSAVPDTNVRNQGEVITQDSVPINNIPEKHTCYAWVESDL
                     "
     gene            10519..10827
                     /locus_tag="PKY63_02600"
     CDS             10519..10827
                     /locus_tag="PKY63_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99535.1"
                     /translation="MKKHISRITYGFARITGWRRLALISGLAFLSACHTKSDQSRVSK
                     DSIPDSVRVKCYEPMELDTASTQAAKDSANKEEMPAVKDSSELKVPLHPCYVPVKPKE
                     "
     gene            10829..11206
                     /locus_tag="PKY63_02605"
     CDS             10829..11206
                     /locus_tag="PKY63_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99536.1"
                     /translation="MKNRITRIFIRMLLSINSVIPARSAVSVFLLGIATMLMTTTTSC
                     GPRVSCYEPSVDSTGTVQMDTTVKKDSIVQSSVNNQANSNSVTDCYDIAIPIDQVVTD
                     SNTNVNKPDDPIKTCYAPVLKPE"
     gene            11215..11433
                     /locus_tag="PKY63_02610"
     CDS             11215..11433
                     /locus_tag="PKY63_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99537.1"
                     /translation="MKKAALRFIYSLTRMTGLRSMALLAGIALLTFSCSAKKEQKSQN
                     SDSSSSDSVYDTCYKSVQPMNSEKDSIN"
     gene            11443..11691
                     /locus_tag="PKY63_02615"
     CDS             11443..11691
                     /locus_tag="PKY63_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99538.1"
                     /translation="MKSVKQFTGHFLLRAIRLIPPKSAAAGFMIGLAALLTTSTTSCE
                     PQVMCYDPAPPDSIPQDTVDSTRQTLPTDIFENADEKR"
     gene            11678..12718
                     /locus_tag="PKY63_02620"
     CDS             11678..12718
                     /locus_tag="PKY63_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE99539.1"
                     /translation="MKKDKTALPHISFETETRCNLQCRYCYNIWKMPGGEFPGEVTYR
                     KARRVLKKLYRRAKVDHFTFTGGEPLLFDRLAELVLFVRLKGSTVTLISNGNAGTEAD
                     FKMLIKLGVGLFEFPFHSIDAEIHDRMAGVEGAHEKSKATIEMVQRLGGRVVAVIVLT
                     RHNIPTLKETLLRLKEMKVASIMLNRFNIGGAGIQWAEDLMPSLQELRDAFALVEKTI
                     VEHKISLTSNVCTPICVLNPKDYPHIGFGHCSSDPKLKPITLDHHGNIRLCNHSPVYA
                     GNIMESSFEEIFSNDYVKSWDEKEPDFCTGCALYADCFAGCRAAAEQMGRTIRGADPV
                     LEMVGAEKPVAE"
     gene            13088..13384
                     /locus_tag="PKY63_02625"
     CDS             13088..13384
                     /locus_tag="PKY63_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99540.1"
                     /translation="MFKKIFFTCIIFLCAISSYAVSQSEDCSGGPDTITVVFEKQFSN
                     KVVKVLHNDSLILKEKAITLRKHGLEVTGVGTQIFVTGEKEKIVVRCGIWSKRI"
     gene            complement(13670..13888)
                     /locus_tag="PKY63_02630"
     CDS             complement(13670..13888)
                     /locus_tag="PKY63_02630"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HPE99541.1"
                     /translation="MKQFSVEVNDDKVGFFIELMKSLRFVRVKEIPSTYDLNQEQKDE
                     LDKRVEEYHQAPSSYQDWECAFSQIRNH"
     gene            complement(14136..14555)
                     /locus_tag="PKY63_02635"
     CDS             complement(14136..14555)
                     /locus_tag="PKY63_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPE99542.1"
                     /translation="MLPMIKNNSFFPSVLDEFFNDVPAFRSSATRWMPKVNVAENDKS
                     YQIELAVPGMEKEDFKIEVKDDVLVISSEKKEEKNEEGKNYSRREFSQCSFSRSFILP
                     DVADSSKIEAAYKNGILDVTIPKKEAEEKLSRVISIQ"
     gene            14837..16756
                     /locus_tag="PKY63_02640"
     CDS             14837..16756
                     /locus_tag="PKY63_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE99543.1"
                     /translation="MNTITELFDTSVRQFGNNPLIWEKADGRYVSTTYTEASVLVNHL
                     AGGLMNIGLQPADKVVLYSEGRTWWLLSELAVIACGAVSVPVSTRIEEADEMLFRMQH
                     SESKFIIISARFLDFVRAIKKENGQTHKIIILDLPNKANIDLQSDEFLLGNLLEIGSK
                     CEKDTIATLADIAAKTSPDDEVNICYTSGTTADPKGILLTHKNYLTNVHQSEAMFDVT
                     PDFITLLMLPWDHSFGHTVGLYTLILKGASMAAVDGGKSAMEALRNAPINIKEIRPYF
                     LLSVPALAKNFKANIEKGITDKGKFASVLFTFFLNTSIRYHADGYNKGKGLRFLLKPL
                     VWLGDQILFSKVRLAFGGRLRYFVGGGALLDADIQRFFNAIGVPMYQGYGLSEASPVI
                     SSNCPRGFKIGSSGKPVPMMEIIIADEDSKPLPTGQSGEILIRGGNVMKGYWKNEQAT
                     KETIVDGWLHTGDLGYLDDDGYLHVKGRFKSLLIANDGEKYSPESLEEAMVYHSDIIS
                     QIYLHNNQNPFTTALVFLNFEKLKQKLAAVGLSIGNEGHRTGILELITKEINLLRTDP
                     VLSRQYPSRWLPSIYCIIEDGFNEKNHLLNSTLKIVRPKIEATFKGLFDMMYGQEGKK
                     KTDDYNMAILEKTQQ"
     gene            16803..17339
                     /locus_tag="PKY63_02645"
     CDS             16803..17339
                     /locus_tag="PKY63_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPE99544.1"
                     /translation="MKKSVILCLILGILVQTLISCGHQKSQAIDNDFEVVADTMQSKI
                     TPKVQGTITDYEKLFTEGESKTLVNRCNEFEAKNAIPVGVFTCDNIGDFNNFTEYADA
                     VSTKWNGCKDNQGLLFVISNKLGEIRMISCPATENRITDNDFDFVINTVIFDAFRKSQ
                     FFDGIINALDYLDDKIKK"
     gene            17342..17638
                     /locus_tag="PKY63_02650"
     CDS             17342..17638
                     /locus_tag="PKY63_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99545.1"
                     /translation="MPTRVFFNEQPEPFVILNYAEDLSSVCISVSHEDHADPVELFST
                     GCGKDIAEELQSKEGFSVYMEGCKKINLRYRRFLFKKKWRVMYEDVELSGIKVL"
     gene            17635..18333
                     /locus_tag="PKY63_02655"
     CDS             17635..18333
                     /locus_tag="PKY63_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99546.1"
                     /translation="MKAFLFQVVFLLSVFIQPLSAQNNKFKCPWDEIDCPGKCGRFFD
                     ADGDGFCDYGRVSKVEKKDTIVRKDTVAIKKENVAGTNVIPAEESSKVIVDADSDSVQ
                     LESEAVPEIKNEIKTPSKARSYSLLLITLLCFGFYGFTSFLMELKLFRKFVHRRIWNI
                     LLLLTFLTTAVLGIVLVVQINYQLDSSLIRDYLYWHVQFGIAMSVISVIHIFWHWKYF
                     YRIFSKKSKSDCVN"
     gene            18378..19442
                     /locus_tag="PKY63_02660"
     CDS             18378..19442
                     /locus_tag="PKY63_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99547.1"
                     /translation="MIAGLQLRAQNLVPNGSFDDKKGTRPTMKPWKKLNTVDFFVNAD
                     DRRYKEVNAARNDRNYILRKPRSGHAYVGIRVWPKYNEYLQIQLRRPLTEGKAYSFEM
                     YVTPSRYSNCFLKKIGVSFYPNRPPYTTRAGREDFPPQVETYRPFGVRDTAEWVRING
                     VFIAKGGEKIMTIGNFSTTPRDKFKRKKLSFKKREAYYYIDDIAVYELDEKGMPILDS
                     ITYALAQQDSLDSDSADFIASTFAGLDSNITNIYFEENSAKLDEAGYKKLGIITEFLF
                     ENPGATIRIETYASPGEMDGQEMKLSEKRAKSVYIFLSGNKIEKDRIVIRPVGNSCEF
                     YKAGDPKRRFCRKAEIIILQ"
     gene            19457..20521
                     /locus_tag="PKY63_02665"
     CDS             19457..20521
                     /locus_tag="PKY63_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPE99548.1"
                     /translation="MKKILVVFLALLIAGALQAQEPFKISKDIPHTPVKNQQHAGTCW
                     SFATVSFIESELLRSGAGEFDLSELFFVYYAYLMKTENFIRLRGLANFSQGGQAHDVF
                     AVMDKYGAVTEDFFNGYAPGDTLYDHSEMEKILSAMAKVWTGKDGAGHHWKSAVRAIL
                     DVYMGVMPTQMTFRKKTYTPMEFRQAFLPKSADYIELTSFSHHPWFHPFRLEIPDNWM
                     GAQYYNLPIDDLMSVIDYALENGYTVCWDGDVSEPEFKHRQNYARLENENIKLSQDLR
                     QKDFDNFSTSDDHLMHMTGLSQDSAGNRHYIIKNSWGDSNSEHGFLHMSKGFVYMKTV
                     AILIHKNAIPEKIRTKLGIN"
     gene            20732..21400
                     /locus_tag="PKY63_02670"
     CDS             20732..21400
                     /locus_tag="PKY63_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99549.1"
                     /translation="MRVVILIFFLFIVGSMMAQSESQVMSLSDSQTYGVVSDKSLKKN
                     VDSIIPAHIIIDPAQGVAPLAVQISTPAPALSCTWIIEGMTFTDISAFSHVFQHAGSY
                     DVCLFTLNHGGFKDSACSVVQVWAGSQQDSSFLVVPNVFTPNNDLQNDFFRTTSLNIV
                     EWDSRIYNRWGQLVYNSTAADVMWDGLNNGTPCDDGVYVFIIKAVGGDGKLYDISGTL
                     TLFR"
     gene            complement(21482..22621)
                     /gene="rffA"
                     /locus_tag="PKY63_02675"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(21482..22621)
                     /gene="rffA"
                     /locus_tag="PKY63_02675"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HPE99550.1"
                     /translation="MNIPFNIPYISGQELFYIKTALQSKQCSSEGIFIDRVKEWFAIN
                     YKTPSVFLTTSCTDALEMAALICKIQAGDEVIIPSWTHVSTANAFTLRGARIVFADVE
                     AGKMTLDVNDVISKISDKTKAIAAVHYGGYSADVIHLKKICDSKNIFLIEDAAHSIGA
                     GIDGHKQGSIGHLGCISFHESKNIHCGEGGILLVNDKNLIETAEIVFQKGTNRSMFLR
                     GEVPFYEWIQAGSSFSMSNLTAAMLLAQLQETDNVNYRRLELWKSYYELLGNIDGFAK
                     YFSLPPLPDKEDDFNAHIFFIKGNSENISAKIHRALNNAGIQAQFHFPPLHTSLFGKR
                     FSGNCPVTASENIRLLRLPLFYGMNSNQIKKMAIILGKILKKIRL"
     gene            complement(22618..23595)
                     /locus_tag="PKY63_02680"
     CDS             complement(22618..23595)
                     /locus_tag="PKY63_02680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPE99551.1"
                     /translation="MKLNVALLAGGYSGESVISVQSAGVIAQKINREKYNVYLIEINK
                     ERWCWKNNNETFDINRHDFSLQTPDRTIHFDVAFIMIHGTPGENGLLQSYFELLGIPH
                     TTCNAMVSNVTFNKFYTIQLVKALGLTTSRSVYFNNEASARPEDVFTHLNFPVFVKPN
                     SGGSSIGMSKVKTAEEIEGALEKAFREDKEVLVEEYTMGDELTCGVLRINSEITALPV
                     TLVKSKKDFFDFEAKYTQGMSDEITPAPVDQKKTDQVVAASKMLYEKLNCRGVVRFDF
                     IHNGNEPVFLEVNTVPGMSSASIVPQQAQAAGIQETELYDLIIENALKY"
     gene            23719..24444
                     /locus_tag="PKY63_02685"
     CDS             23719..24444
                     /locus_tag="PKY63_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPE99552.1"
                     /translation="MAKYNKWVFGALGWAFIGPIGGLFGFVLGAYFDYASQEKLHTNT
                     EGATTPGDFRLSLLALLAAVMKVDRKNLKVELDFIKQVFNNTFGAQYTKHSMPLLKEL
                     LNKEIPVRDICFQVRTHMDHAARLELVNVLLSLCMVDQELHPAENELMKKISMWLGIS
                     DKDYQSMHSVHIPDTGWAYKILEVKPDASDDEVRKAYRRMAMKYHPDRVAHLGDDVAK
                     SAGEKFKAVSKAWDEIKKERNIN"
     gene            24507..27707
                     /locus_tag="PKY63_02690"
     CDS             24507..27707
                     /locus_tag="PKY63_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPE99553.1"
                     /translation="MRKIVMICLLFFAVLPLAKAQELRGGLPFFVNYHPSEYRGYSQN
                     WVLTSNSKGFLFVGNGDGILMYNGRTWSKFLLANSATPASFFCDENDVIWVGAQSELG
                     CFLPDNTAGYRYVSLTHLVRSKFREFGFVFSINKTPDGIYFRTAENIFRLVPQGMYVI
                     PVERGSHLFEFRHTLFLYEPSKGFSVVSGNKLLRVISELPEQMVIRKVITLGRDSALI
                     VDSQHGLHKVWFDFTNRYAIKMLLKPLSVPINDFLVSNEVFHAIRMTNGNYGFATTRN
                     GTIVTDSCFNILYHLSREAGVMNETHTYLYEDRQHNMWIAMDNGICKVNAQSPLCNFD
                     DYQGVKGSVLDVVRCFNQIFIATWQGVFYQNRAEDRSKNEIFLPLPQIASQSWDLEPI
                     VVAGKCYLLAATSDGVFLIDSTLKTTRVSDGNFNYVKARSDNNQLVYAGGPLIIKYLD
                     FSKSITKPKETQVPNLESRIINAVLSENNQLFVGTARDGVFVFSAVPLSGSGDDMVFE
                     MSQIGVRNGLPESDQYSVFRFRNQTLVSAQNGLYRIVQKDHHYSVIPFDREFFTFYNK
                     SQFINILKEDDAGNMWFQTNSKLTGHKSLYYVKWSGSKKEFISSPFMAFPDLEFYSIT
                     PEGDSVVWLGSDDGVFRFNWKVSNSFSFAEPFTTSIQKFSLNGETLYSRVARNYLVSE
                     PDVVAFNKGNIRFDFSAGYFIHEGQVQFIYMLEGFDDQWSEPSSENYKEYTNLPPGEY
                     TFHVKAINPFGAEGVAADFSFVIPAPWYNNWWAWVAAAFLAALLVVGIVTYFNRRLIK
                     AKLRLENIIHSRTLEIQNQKKAIEIEKEKADKLLLNILPVRIADELKSTGRCQTEFYQ
                     SVSVLFTDISGFTSISELMDPEELVSKLDEIFTQFDDICSRNKLEKIKTIGDSHMSAG
                     GIPVRNRTHAVDATLAAMEMQQFMEKMRQRNPADSIWKLRIGINTGELTAGVVGKRKF
                     AFDIWGDTVNTASRLQDAGESGKINISNRTAELVSSFFDLTYRGKKPIKHKGEVDMFF
                     VDGIKAELSVDGKKLRPNKAFWDKYNELLELRFLNF"
     gene            27710..28294
                     /locus_tag="PKY63_02695"
     CDS             27710..28294
                     /locus_tag="PKY63_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE99554.1"
                     /translation="MDFERVKDYMMQRLSRGLAPNLYYHGIHHTVDVMRAAEEIAAAE
                     RVVGMDKTILMTAALFHDVGFLIRYKENEDISAGMCWNILPRFNYTQSEIMMIAHIIM
                     STSIPQKPVDKLDSIMCDADLDYLGRDDFFIIGCNLRREWKEYGRELTVKEWYFQQID
                     FLSAHHYFTKTATLLREEKKLRHLDYLIRLVNKK"
     gene            28357..29211
                     /locus_tag="PKY63_02700"
     CDS             28357..29211
                     /locus_tag="PKY63_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE99555.1"
                     /translation="MESRNKIVIVPFDFTDVAECAVNHAAGLAKHQGWTVQLLHIINR
                     DTRALLNVYKVTDDIVKERLQEKVDEFSNKYGVPFSFDAKEGSIFTDIGKTAEDVGAN
                     FIVMGTHGKQGIQHIFGSYAHKVITSTRIPFIIVQKRDFREGYNRILIPIDDTLESRQ
                     KIKWAIYIAKQFDATIYVKAMYLNDELYKAKIRAVLNKVVEVFEKNEVKYEAEIIPET
                     GSFAKQVIDYARKIDADMLLVMTNTDSSIVPNFILNPWDEQIIFNDALIPVMAINPRE
                     INITVVGL"
     gene            29283..30818
                     /locus_tag="PKY63_02705"
     CDS             29283..30818
                     /locus_tag="PKY63_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPE99556.1"
                     /translation="MNPKNLFSETDIRQIEEHGLTVLQVENQLAGFRRGFSPVKLIAA
                     ATNNNGIVSFSQNEQKKLIALFEENASDYFMARFVPASGAATRMFKEIFAAVDALNNH
                     WDMPMPAEKFLENLKRFAFYDALKDVLKKHGHDIDSLIENSVWLPVLDAVLKKDGLNF
                     ASKPKAFIPFHQYKGFTRTALEEQIYEGLTHAVGHHNTMNIHFTISPEHLKEFKKQTQ
                     KCLAELKAKCDINIKISHSFQQKSTDTVAVDMDNIPVRDSNERLVFRPGGHGALLQNL
                     NDIKAEMVFLKNIDNIVPDWHRNDTVWFKKVLAGYLMQVVNDIHAYLIMLDNGNVGNE
                     DLNEMIVFASDKLFIEIPDWLTEADPLEKIDFLFGVFNRPVRVCGMVKNEGEPGGGPF
                     FVEDSNGNISLQIVESSQVDISNADQKKILLSSTHFNPVDLVCWKNDFNGRPFNLNDF
                     SDPETGFIAKKSFQGKDIKAMELPGLWNGAMADWITIFVEVPLSTFNPVKEINDLLRE
                     MHQ"
     gene            31150..31629
                     /locus_tag="PKY63_02710"
     CDS             31150..31629
                     /locus_tag="PKY63_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99557.1"
                     /translation="MPKIASIIFLLLVSAISNAQTNSLEFHNRIVAQLSRIDIAVSRF
                     NEGIVMYDGDTILARYKNLLSETEASIAETEKISIASGGEEYHKAAFELFRFYLRLFE
                     NDYYDAVAIMYRIPITDADIEEMERLFDRIELKESIFLNAFFNAQIDFCEAAGIEIE"
     gene            complement(31902..33467)
                     /locus_tag="PKY63_02715"
     CDS             complement(31902..33467)
                     /locus_tag="PKY63_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99558.1"
                     /translation="MKTKILAVFLLIISTSLSAQINTTLNRNYGGPGIDNITRFYQAS
                     DGGFFWCGYSNAAGGDIPQINGAEDAWFMKVDANGDSLHSVAFGGLANDEIADVMEFS
                     SPDYVFLINSNSDDNGFAGGHGGIDTWIKGYDPAVGLSSGAAFGGTLHDFGAKISPKI
                     SGGYIVSGYTFSSDGNLSGHYGMSDIWVMSLQSNLSVAWSRHYGGTDDDRAIAAYQLG
                     DGNIMVFGTSASTNHDVHDANGNNDVFVMKLNSLGDTMWTKTYGGSSTDLIVDAKYLN
                     DSTFVMIGYSNSNDGDFMFRDKVLAYYGFYYVIDENGDFVSCGSQNTLDNKDLFFSDV
                     IYEGPNQAMAFGTTISDSLLNCTTPTHGSLEVCAVDFNGLTSITPYLLGGNNIDGMNS
                     DVEYYVRALKTGTNQYVLCTNSNSTDLSADYHGLYDVWLSFIEVQHVSVPEAPQLQLS
                     VYPNPAENIIMIENITAGNDSQFEIYDMQGKLLLSGQYSESGIDVSSLEHGMYLLKLQ
                     SGDSTGIARIVKQ"
     gene            33829..35205
                     /locus_tag="PKY63_02720"
     CDS             33829..35205
                     /locus_tag="PKY63_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPE99559.1"
                     /translation="MNLPYAEPYKIKMVEPIHISTREEREKWIADAHFNLFNLKSEQV
                     FVDLLTDSGTGAMSDRQWGAIMTGDESYAGSRSYYRLKDTIHEILGFPYFMPTHQGRA
                     AENVLFSVLVKEGDVVPGNSHFDTTKGHIEFRKAHAIDCTIDEAFDTENLHPFKGNVD
                     INKLKKVFETYPHDKIPFIIVTVTNNTAGGQPVSLENLRAVKEFAKSVNKPVLFDSAR
                     YAENAYFIKVRENACASMSIREIVREMYKCADMMTMSSKKDAIVNIGGFIAMNSEELW
                     QKVSVFNIMFEGYVTYGGMAGRDMEALAVGLEEGTDFEYLKSRISQVEYLGNQLIEYG
                     IPVQKPIGGHAVFIDALKFLPGLPREQYVAQTLAIELYLEAGVRGVEIGTLLADRDPE
                     TRMDRFPALELVRLAIPRRTYSNNHIDVVAAALKNIWDRRSTITKGLKIDYEAPIMRH
                     FTVKMSRV"
     gene            35353..36636
                     /locus_tag="PKY63_02725"
     CDS             35353..36636
                     /locus_tag="PKY63_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99560.1"
                     /translation="MKKRLFLFSFFFFLLFFLKAQTDTLTVMQYNLLNYNNYTSYCTS
                     VNNNVSNKDGYLKTIVDYVLPDIFTVCELNQLQTSVDRILTNVMNTSGRINYAKGART
                     NYSSSDLINMVYYNTDKLVMHSSTALPTDIRDINLYTFYYKDPNLAVTHDTAWVTCII
                     MHLKAGNYTGEPEERLAEVTILMDYLDAVNKPGNYLAMGDFNTYTSDEECYQLLLNHF
                     NTNIRFYDPISKPGDWSNNCSFASYHTQSTHNDFDNDCFAAGGLDDRFDHIMTSLDVM
                     SGGKNVKYISGSYNALGNDGAHCNDSLNEASNTSVPANVLAALMGNSDHLPVILELQI
                     DVETGIHDEAKVPFDFYIPSNIADDYGILVSPCSGRYSIEAMNVTGSLICRFQSDAGV
                     GFNTINLSAVPKTASIAFFRITDPSGNVKVYRTLR"
     gene            complement(36768..37232)
                     /locus_tag="PKY63_02730"
     CDS             complement(36768..37232)
                     /locus_tag="PKY63_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPE99561.1"
                     /translation="MKEYRFTAKIIKNPNVNSGYIEFPHDAEKEFGRKGQIKVKAYFD
                     GFEYRGSLVKMGHPCHIIGLNKKVRDAIAKGPGDTVDVLIIEDIEERVVEVPADFEAA
                     LNKNETTKGIFEKMSFTHRREYVEWIISAKKSDTRENRINKAIEMLLNKKAK"
     gene            complement(37599..38228)
                     /locus_tag="PKY63_02735"
     CDS             complement(37599..38228)
                     /locus_tag="PKY63_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99562.1"
                     /translation="MKNIFILIFVVSVFAVACNHSSKNNSDQELKTDSTAASLKDSVS
                     DEDLIQDAKMIVQHFKNKEFSQLAEYVGPQGLRFSAYAFIDTSKDVVMSAPQIRNIAT
                     SSEKLLWGEYDGSGEPIKMTIEQYFADFVIDADFLKKGEISINEFLQRGNTINNIREA
                     YPDCKWVEFFIKGEDPRYDGMDWKALRLVFRVGEGKAVLVGVVHDEWTI"
     gene            38832..40169
                     /locus_tag="PKY63_02740"
     CDS             38832..40169
                     /locus_tag="PKY63_02740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE99563.1"
                     /translation="MTFDDFSLIEPIKSALATEGYTTPTPIQSEAIPVALKGRDILGC
                     AQTGTGKTAAFAIPILQLLNQQHQSGVDRKTIKVLIVTPTRELAIQIGDSFTAYGRNL
                     NIKHTVIFGGVPQNAQVNALQRGVEILIATPGRLLDLMNQKYITLKNINTLVLDEADR
                     MLDMGFIHDVKKILAAVPQKRQTLFFSATMPPEIVKLSSSILNQPVKIEVTPQSSTVD
                     IIEQSVYFVAKVDKPSLLMHILKDNSLETVLVFTRTKHGADKVVRILNKSNIKAEAIH
                     GNKTQNNRQRALNNFKSKVIRVLVASDIAARGIDIEELSCVINLDTPNIPETYVHRIG
                     RTGRAGAEGIAISFCDVDEKPFLKDIEKLIGKKIPVVTNQPYPLDPNAKIERTERPQR
                     PERNQRSDRRPQQEKKKQSSHETRPSREQGQKRPEGFRKQSDNHSGQRVLVKR"
     gene            complement(40389..41384)
                     /locus_tag="PKY63_02745"
     CDS             complement(40389..41384)
                     /locus_tag="PKY63_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPE99564.1"
                     /translation="MSSSFAMHVSSTGKADVLPAAPELDSDSLYIWHHFFQPTEEELK
                     KLPGEYGLHPLAIEDCMDDDQIPKMESFPKNTHLIFNTFNYHNEDIHAEEVNLFIGDK
                     FIITVTRSERLNESIITLTKKMSESKNGLWRNGPGWLLHYIIDKIVDEKMDALEALED
                     ETDSIEDLLLSRTDDIDFAKIQKLRYAFQLLRKSLFHEREILNRIIHDDHDFIDNKAA
                     YLYRDVYDHVVRLYELTESCRETVKNLIELHLAMANNQMAIASENTNKTIRRLTFITT
                     IFMPLTLIAGIGGMSEYSMMTGPENWRIAYLILVIILTIIGILSYRWLQGRDKES"
     gene            42015..42653
                     /locus_tag="PKY63_02750"
     CDS             42015..42653
                     /locus_tag="PKY63_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE99565.1"
                     /translation="MEKTIMEAAEKLFLEKGFAMVSTTQIAKAAGCNQALVHYYFRTK
                     DRLFEAIFAEKLKTFFSVIFQPQDESLTFEEKLKKYISAHFDMLLANPKLPFLVINEL
                     TTNADRIDSLKEMLGQSITYVYGPFQQELDAEIAAGRIRPTTIPDIIYSMVSLNVMAF
                     IGAPIMKKVLFLDEKHFKAMLLYRKEENIRIILTGLKPDVEIKSEKKTKKRK"
     gene            42656..43915
                     /locus_tag="PKY63_02755"
     CDS             42656..43915
                     /locus_tag="PKY63_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE99566.1"
                     /translation="MSTRLLFLLLLCTVLSYTSHAQLTPEQCYEKARANYPLVNQYGL
                     IEKSESYNLSNAARAWLPQVSLSAKATWQSEVIELPFTMPGIGEMDQDQYQATLEVSQ
                     ILWDGGSTSARSAMYKASADIEKQKYEVDMYALQERINQLYFGILLLNEQLNLNAVLQ
                     KDLETNYNRIMVSLNNGVASQADLDAIRVEQLRARQMQAELQASKKAFTDMLSAFIGE
                     QISDGTVLSKPVAGLNASDIYEIRRPELQLFEAQRTFYNSQQKLVRSVSMPKLALFAQ
                     GGYGKPGLNMLSNEFNPFFIGGIRLSWNLSSFYTQSNEMQLLKINQSGADVQKQTFLF
                     NTNLKVSQINTEIEKFTELLKSDDEIIRLRQGIQLSTEVKVENGTASASDLIRDLNAV
                     NRSMLDKALHEIQMLQAIYNLKNSTNL"
     gene            43927..44829
                     /locus_tag="PKY63_02760"
     CDS             43927..44829
                     /locus_tag="PKY63_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPE99567.1"
                     /translation="MKAIHYLLALVMIISITSCGNSNGNTDATGVFEATEIIVSSEAN
                     GKILELNVNEGDELKKNQLIAVIDSTQLYLKKLQLEASAKGVRSRNPNVRVQIAATQQ
                     QLETARSEKKRIENLVNANAINSKQLDDINAQILFLEKQLAAQKETLENTSSGIGSES
                     EAINIQIAQIADQLHKCRILSPIDGTVLVKYAEQSEFAVTGKAILKMADTEQMFLRAY
                     VTADQLTKIKIGQKVKVLAESGEDDSREYEGMVTWISDKSEFTPKTIQTQDERANLVY
                     AIKVAVKNDGFLKIGMYGGIVLGD"
     gene            44833..45744
                     /locus_tag="PKY63_02765"
     CDS             44833..45744
                     /locus_tag="PKY63_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE99568.1"
                     /translation="MSVVVQNISKKFGLVQALNDVSFSASKGEIFGIIGPDGAGKTTL
                     FRILTTLILADGGKAFIDGFDVVKDYKKIRHLAGYMPGRFSLYQDLTVEENLNVFASV
                     FKTTIEENYHLIRDIYQQIEPFKKRRAGALSGGMKQKLALSCALIHKPSVLFLDEPTT
                     GVDPVSRKEFWEMLIKLKQEGITILVSTPYMNEAALCDRIALIQSGKIFKADTPQNIV
                     RSFGKTLWAARSSNMSKLLNDLKNHPEIETCYAFGDSNHLIMKNESTSGELLKQQLLA
                     KGHEKVDVFPIEAGIEDCFIYLTSIKR"
     gene            45762..46481
                     /locus_tag="PKY63_02770"
                     /pseudo
     CDS             45762..46481
                     /locus_tag="PKY63_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            46483..47589
                     /locus_tag="PKY63_02775"
     CDS             46483..47589
                     /locus_tag="PKY63_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE99569.1"
                     /translation="MRQFLIFTQKEFYHIFRDRWTMIILIILPVVMLILFGYAITTEI
                     RNSHFVVFDPSRDAATQGIVNKLETSEYFVFDGYVHTPAEAEEIFMRGEIGMFIIFSE
                     NFYNNMLHTGNAQIRIVTDGSDPNTAHTLTLYAQNLMASYQQDIFKSMEIPYQIEPQI
                     RLLYNPTMKGLYNTVPGVMGMLIMLVCAMMTSVSIVREKEKGNMEILLVSPMKPISII
                     LAKVVPFFMISLMNFTTIIVIATTLMGVPVNGSWFLLIAVSLLFIFVSLAIGILISTL
                     VSKQMIALLASAMGLMLPAVLLSGLVFPVSSMPIALQWVAQVLPVKWYIEAMRSIMIM
                     GVGFSSVSLQFFILSGMAVLLLVLSLRNFKIRLD"
     gene            47592..48704
                     /locus_tag="PKY63_02780"
     CDS             47592..48704
                     /locus_tag="PKY63_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE99570.1"
                     /translation="MLKYLLEKEFKQFFRNKALPKMMVIMPLMAVLIFPLAADFEIKN
                     INLSVVDHDKSAYSSLLVQHVQASGYFRLTDVSESYGESLKSVEKGKSDMILEIPLGF
                     EKNLVKEHQSDILIAANTVNGMKGGIGSSYLANIVRNFSSDIRLEWVQGNSRLNAPQF
                     EIVPDYRFNPQLLYRVTMIPAIMIMILAMVSGFLPALNIVGEKEDGSIEQMNVTPVKK
                     VYFILSKLIPYWVLGFVVISISFLVAYIAYGLIPVGNLLVIYLYASLFVLAFSGFGLV
                     ISNYAQTVQQAVFMMFFFVITFIFLSGLYTPVENMPEWAQAISTFSPLKYMIQVLRLV
                     YLKGSGFAELTTQLIALICFAVFFNGWAVLSYRKSH"
     gene            49277..51988
                     /gene="ppdK"
                     /locus_tag="PKY63_02785"
                     /pseudo
     CDS             49277..51988
                     /gene="ppdK"
                     /locus_tag="PKY63_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
BASE COUNT        15066 a        10164 c        11607 g        15517 t
ORIGIN      
        1 ttccatctgc ataaatgtgt accaaagctc ctgcctgatt gagaaaagcc gttgtaaatg
       61 atatgccgaa tttaaccgga accatagcaa tgccacgttt aataatctga ttttttgcat
      121 tgaattcatc ggttattttt tttcgcatgt caaaatccga tgaattgaga attttttcat
      181 gaatcagact gagtttgttt tcttttacag aagcaccata tggcgcaata ttgcgttcat
      241 gtattccgta atagttcagt tgacgtattt gattgggatc ttttctcagg taacgagcca
      301 tttggtcaat aacattttca attactgcaa gaccttgtgg ctgaccgaac cccctaaaag
      361 cattgttgct tggaaggttt gttttccaac tgttgccaat aatgcggata ttggggatgt
      421 aataagcatt ttcggcatgc agcatagctc gttcgagaat ggccattgtc agatctgatg
      481 cgcatccggc atcggcatta aaaataatgg tgtaagcttc gatgagtcca tcattatcaa
      541 atccgatttt ccagtccgat tgaaagcggt gtctttttcc tgtgatactt tgatccatgt
      601 cgcgtgtaag tcgcaagcgg actggttttc tggttttggc agccaataat gatgcccatg
      661 cagccaaatg atttccctgt gtttcttttc cgccgaaagc gccaccgagt ctacgtgttt
      721 cgacaaccac ctgattgcgg ccaataccca acacttcggc aataacggcc tgtgtttcgg
      781 atggatgttg tgtgctgctg tagcaggtca tttcgtcgtt ttcacccggg acggcaaggc
      841 atatctgagt ttcgagatac cagtgttcct gacctcctga gcggaattgt ccgtgtaaaa
      901 catgttcgca tgaatgcaga atgctgtctg gatcaccacg ctcaatgcga cgttccggtt
      961 gaagtcgtgc gttgttttga atggcttcat cgatgctgag aataggtgtt tctgtttcta
     1021 tatttagttt tacaagtaat gctgcctgat gagcaatttc aaaagtatcg gcggctatca
     1081 gcgcaatggc ttgccctatg cactgtacct gaccgtcagc cagacaaggt tcatcatgaa
     1141 agactgggcc catctgatta tcgccaggaa tatcttttgc agtgataatg cagcgaacac
     1201 ctggaagtga taaagcattg ccggtttcaa tactttttat tttgcctttg gcaacaggtg
     1261 atgttaccag ccaggcctgc agttcgcctt gcagagccgg catatcgtcg atgtattgtg
     1321 ttgttccctg cacatgtttt atggcgctgt cgtgaaccgg gtcgtgatgg ctgatggtgt
     1381 gtttcagttg ctttttcatg ttcaggaaat taaagtgtca ttataaaact gaagcaacag
     1441 attagctgcg gcaatattac ggaattcagc tccactgcgg gcgtcggaca atggcgtaaa
     1501 tgattttttc actatgttca tggcctcatg aatagttgat gcattccatt ttttgcctgt
     1561 cagaaaggat tctgcttctg ttgccgggat tgtcatagct gccattccac cgtaatatat
     1621 tgaaacagct gtaacgactc cattgtcaag agtcaggttg aatgctgcac tgactgttga
     1681 aatatccatg tcgttccgtt tactgacttt atacattttc acaatttcat tttctttggg
     1741 tagaggcaga tgtatttcag caatcaattc atcggcttca agaattgttt tacgatatga
     1801 aacaatgaaa tttctcaatg gaattatgcg tgaatttatt tcggaaacaa gcttcagatc
     1861 tgcgttacag gccataagta ggggcgttgt gtcgccgatg ggcgatgccg atccgatgtt
     1921 tccacccagc gttgctactt gtcggatttg ccttgatgca aaaacagata gaatgtcatt
     1981 aaatgccgga aagacatttt tcgtagcaat gcggatgttt tcgagcgata accctgcacc
     2041 gattttcagt ttgttttcag ttatctcaaa agttttcaat tcattgatgc ctgaaatatc
     2101 gagaatggcc ggaatatgtt taaaatgctt gctttgttgt aatgctatat ctgaagagcc
     2161 attgaccact attgcttccg ggtggttttt tctcactgta agtgtttgct caagcgaaat
     2221 gggtctgatg tataattgct gttggatttg aatttcgaac tgactatagg gagtaatgga
     2281 attgagtttt tcaattaaaa ttttctcttc tttgtcaaat gcatccggat gtcggttctg
     2341 catgacagct tttgctgctt tgcgtatgct gtcatagcca gtgcatcggc aaagatttcc
     2401 ggccagggca ttattgattt cattatcatc agcaggagcc gggttgtgat attgagcaaa
     2461 aagactcatg acaaatcccg gcgtgcaaaa accgcactga gtggcgtgtt catgaactac
     2521 ggcctgttga acgggatgta actcatcgct gtaaatagtt tcagttgaca gaccgcgtga
     2581 ggtaatgagc aattttccat caatggctgg cagaaataaa aggcatgagt tcacggcctt
     2641 gtattcaagc gctttcccgg tggcatcggg agtagcgata acaacggtgc atgcgccaca
     2701 atcgccttca gcacaacctt ctttgacttc ggtatggtgc atatctttgc gcaaccagtt
     2761 taatacagtg gtggaaggtt tgatgttgtc tgcggcaaaa tcaatccgga taagctctcc
     2821 gttgagcaaa aaggagatat gctgttttgt tttcatcgat tgtttttatt tttaacatca
     2881 atcagtttag ccagaatgga gaccgctatt tcttcaggag tttcgcatgc tatgtcaatt
     2941 ccgataggca tatcgatgga atttattttg tttgacggca tgctgaattc ttcagaaaat
     3001 cgttttcgtg ccgtatcaac tttgcgtaca gagccaatca tgccaatgta tttatgaggt
     3061 ttatcaataa gcatggccac gacttcgctg tcgaaagaat gtttgtaagt ggttactaca
     3121 atgtaagagt tgctgtgaaa ttttattttt tcagcagctt cttcaaaagg catttcatat
     3181 gttgttgcaa ggttctgagg ccatgttgca taaattcctt cgcggttgtc ggccacggaa
     3241 attttaaatc cggatattga agccagcttt gcaagcgctt ttccaacatg cccggctccg
     3301 aaaataatca gttcaggttg ttggagcagg ggttcaaaat aaacggtcat gcggccgcca
     3361 cagtgcattt caagatcgtt ggcaagatta aatgattgta cggatggagc gtctgttcca
     3421 atcatgtttc gggcaagctc aatagcttcc ttttcgatgc tgcccccgcc aatagtgcct
     3481 ttgatggagc cgtcggcaaa gaccagcatt ttggatccgg gcttgcgtgg agtggaacct
     3541 tcggcgccga tgatgatgca taaaactgcc gcaatgttgt tgttgatggt ttcggaaatg
     3601 tcgttgaaaa aattcatggt gctattattt gcaaggtaaa agtacaaaaa aaatcagaag
     3661 agattggtga atggttaaat ggattaaatt actaaatgat taaatgaaca atatggttaa
     3721 atggttaaat tgttaaatgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 gttaaattgt taaatggcta aatggttaaa tggttaaaag atgcttagtt tttttgcgtg
     3901 attttgtgtg aagtaaattt gaaaatacag cagtcatgag ttttaaattt tcatgaagcg
     3961 tgatatacag gttttgatta aaaactcata actgacgatt cacaactgac cactattatg
     4021 tatctttgca ctcgatgttt aaggagtttt tattgcataa ggttttcggc attatacatc
     4081 aacgagcggt tgaaaaatcg cttcgatgct atgttgtagg aggttatgtg cgtgatattt
     4141 tgcttgagcg gccatcgtgg gatgttgata ttgttgttga aggtgatggc attgagctgg
     4201 ctgaatacgt agcatcgcag ctgaagccgc atccgaaagt gtcggttttt aaaaattttg
     4261 gtacggctca ttttgtttat gatgaaacag aatgggaatt tgttggtgcc cgtagcgaaa
     4321 gttatcaacg tgggagccga aatcctgaag ttatacctgc aaccatcgaa gaagatatgt
     4381 tgcggcgaga tttcaccatc aatgccatgg cagttagtct gggcgctgat ttcgctgatt
     4441 tgcttgattt gtttcatggc cagaatgatt taaaaaaagg cgttatccgc acaccgcgtg
     4501 atccgcagat tactttttcg gatgatcctt tacgcatgtt tcgtgctgtt cgttttgcct
     4561 ctcgctttga tttcgaaatg gcaccggaag tttttcaatc aatcagaagc aacattcacc
     4621 ggattggcat tattgccccg gagcgtattt cggaagagct gaacaaaatg ctgctgcata
     4681 aaaacccatc agtagcattt tatcaacttg aaaaatgcgg tttattgcaa tctgtattgc
     4741 ctgagctgac tgcattaaaa ggtgttgaaa acatgcacgg aattttacat aaggataatt
     4801 ttacgcatac attgggcgtt gtggataatg tggctgctgt ttcagaatcg ctctggctgc
     4861 gttgggcagc actgttgcat gatatcggga aagctccgac caaaaagttt gataaggtac
     4921 gtggctggac ttttcacggg cacgatgcag ttggacagcg catggtgctg aaggtattca
     4981 agcgcctgca tcttccgcag aatgaaaaaa tgcattatgt tgcaaaaatc gtaggattgc
     5041 atttgcgacc cattagtctt gttgaagaag gtgttaccga tagcgcactg cgccgtttga
     5101 tttttgaggc cggcgacgat ctggaagatt tattgattca tgccgaagct gatataacgt
     5161 cggccaataa gcaaaaagtg aaattatatc tttccaattt tgcggaatta cgcgaaaagc
     5221 tggcagaaac agaagagaag gacagaattc gtaattttca gccaccggta agtggtaaag
     5281 aaatcatgga agtgtttggt atcgggcctt cacgagaggt gggcatcatt aaaaatgcca
     5341 ttaaggatgc catactcgat ggtgatattc ataacgaccg gtctgaagct tggcaactga
     5401 tgctaaagca aggagaaaaa ctggggttga ttccgcgttg aaaaaattca ttatctcaat
     5461 gtgcacttgg ttcttttttg tatcttcgta caaaattttc gttgtatgca tgcttacaaa
     5521 ttcagagtaa cgtcggatga aaacgatgaa tttctgcgtg aaattgaagt tctggccaat
     5581 cagacttttg aggattttca taaggttctg gtgagaacat gtaaattggc cggtaatgaa
     5641 ttggcgtctt tttatctttg tgataatcag tggagaaaac gtcgtgaaat aacactgatg
     5701 gatatgaata tggaatcgga tgaagatgaa gaaaaaaaat ccgaagccaa tctgataatg
     5761 aaagatgtaa aactcaatca gattatcgat gatcctcatc agaagctgat ttacgtgtac
     5821 gattatttga atatgtacat ggttaatatt gaacttatca gaatcggcca agctgatctg
     5881 acccttcatt ttccgatagt ttcaaaagaa gtttctgata ttattgcacc gccttcaaaa
     5941 ataaatcgtt tagttgagga tgaggaggtt gtttttgacg aagatgaact gcatggcagc
     6001 gaagaagaag gtgatgccga tgatgaaaat cttactgagg aacagtttta cagtgatgat
     6061 tcgtcagata tttaatttaa tttcaatgca agccgatcat ggattcttcc gatagttttt
     6121 ttagtgaaga agaaattgaa tcgctgttaa tgcgtttcag aaatgctgtt gcgcaggata
     6181 atactgaata tttcgagccg gatgaacttg aagccattgc cgatgcattt cttaaacgcg
     6241 atgatcttgc cctggccaaa gcagctattg attatggatt tgaaatgtat ccggataatg
     6301 aagattttca gatcctcaat attcattatc atgttcttga taatcgatcg gattcggcag
     6361 aaatgcttgc gcgaaatttt atagacaaat atcccgaaaa cgcagaggtt tgtcttgttt
     6421 tggcgtctat tctgagcgac cggggcaatc accgcgaggc acttgatatg ctggaacatg
     6481 ccagggagtt ggatgctgag tcggagcatg ccatcacagc actgagtatg gaatatcagg
     6541 tattggggca gcatcgcaaa gccattgaat tgtatcgtga attactcgag cgtaatccgg
     6601 atgatgaatt tgcgctgaat gagcttgttt ttgtgtatga agtggccaat gaaatcactg
     6661 aggccattcg ctttctgaca gattttaccg atcagtatcc cttcaatcag caggcctggt
     6721 ttcatttggc aaatacctgc aatatcaata atgagccaat gaaagccatt gacgcctatg
     6781 attttgcact ggccattgac ccgtcgttta tgccggctat gtttgggaaa ggacattcgt
     6841 acatgtttgc cggcgatcat ctgaaagcca ttgattgctt caaggaaaca gctgagacag
     6901 atggatttaa ggcggcatcc tacctttcat tgggtgaagc gtattcgcaa atgaacaact
     6961 atggcctggc atcggattat tatcttaaaa gcattgaatc cgatccatct ttcaacgaag
     7021 cccgaatggg acttgcctat tgtttgtata tgatgaacct cgacgaagaa gcgttgcgtc
     7081 atgttgatga agcaatactc aatgagccgg aaagatccga tgcctatcag ttgaaaggaa
     7141 ccatactgat gaatctggaa agatatcccg aagcggctat tgaatttcgc agtgcaatcc
     7201 gtgccgataa ggaaaacgat gcggcctggc tcgatttgag cgaaactcat catttcatga
     7261 atggcgtcag cgatgctttg agtattttgt atgaagccga agaagtcctt gaatcggata
     7321 atgcgatgat tgtttaccgc aaagcggcat tgctttttga agctggtaaa gatcaggaag
     7381 ctgtttcgca gttgttgaca ggattgtcaa tcaatgctga gttgtacact tccatttact
     7441 cttacaacaa agaactcgag aataacgaga ttattcagga aattgtaaca atgtttttaa
     7501 atcagtaaca tcatgatgaa ttttgaaatg tcccatttgc ccgaaagaac cgtgaaacca
     7561 cgcacttatg gtgtaaacat ggtcatggac aaagggctga gtttgcgcga agccgaaaat
     7621 atgatgcaag caagtggtca tctgatcgat tttgtaaaac ttggttttgg tacagctgtg
     7681 attaccggcg gtcttgacga aaaaatcagg ttttatcaca atgccggaat tcgtttgtat
     7741 ttcggaggta cacttttcga agctttttta atacgcaatg atttggatgg ttttaaaaga
     7801 gtattggata aatacaaaat ggacaccctt gaaatcagtg atggttcgat ggatttggag
     7861 catgatttga aatgtggcta tataagcgaa tttgcaaaga atttcactgt tctcagcgaa
     7921 gtgggatcta aacgaacgga tgtgattctg acggatgaac aatggcttca ggaaatgcaa
     7981 aaagaacatg ctgcaggatc gtcgtttgtg attgcagaag ctcgtgaggc aggaaatatc
     8041 ggcatttatg acagcaccgg tgctgcaaaa aaaagtctga ttactttgat taccgagcat
     8101 ttcgacatca ataaaatttt gtgggaagcg cctcaaaaaa atcagcaatt gtatttcatc
     8161 aaggagtttg gcagcaatgt gaatcttggg aatattgcac catcagatgt aatcccattg
     8221 gagaccatcc gcctcggatt gcgaggtgat acgttcttcc actttttacc tgatatgctg
     8281 aaacagcatt gatttgtgca gggatttaca gctcacaatt tctgaaatat gaaacattac
     8341 gccattatag gaaaacctgt ttcacactca ttatcgggac aatggttttc taaacatttc
     8401 caaaaaatgg ctatagatgc tgatttcaga atgattgaac cgtcggttga cgagatgaat
     8461 gatttccgca actgggtttc aggcaatcag tttgacggac ttcttgtgac aattccgttc
     8521 aaaatggaag tcatgaagtt tctggatgaa accgaaaagc tggcaaagac aatcggagca
     8581 gttaatgtca tccatgtttc agataaaaaa cttatcggat acaataccga tcatcttgca
     8641 tttgaagcgg agatgaaaag gctgcgtccg gcaggattca aaaaggccat cctgatgggt
     8701 ataggtgggg ctgccgcagc ggtttccatg gcgcttgaaa gaatgagtat tccggtaatt
     8761 aaagtgtcgc gacaaaagaa aaccagtaat attttataca cagaccttac tgagaaaatt
     8821 gtacgagatg ctgatctcat catcaacgca acaccgcttg gcatgggcaa tctgaagaat
     8881 gagtttcctc cggtcaatta tgattggatt cagaaaaacg ctttggccta cgatctcata
     8941 tataatcctg cagagacact gtttctgaaa aaatgcgccg aagttggctg tcagactgca
     9001 aacgggcagg gcatggtgga gtttgtgtat gagcgtgcgc ttgagatctg gggattgacg
     9061 cataaggcat gaggcatagg gcatgcggta tgagatcaga cgcggaatag cgcggaacaa
     9121 ttgaagacat ttctgataaa cgcggcgctc aatgcacaat gcatggagct ttaggcttaa
     9181 agctgaatgc ttaaatttgt acaattcctt aagctttaca actttaagct ttaagctaaa
     9241 accctctcag ttccttcttt aacatctctg cgtcgcgaag cgacctgagt gcgttaaaga
     9301 agcacgatct tctccgctcc ttatcagagc tgatagctga aaactgattt ccaacttgtg
     9361 aattttccaa aaacattcat ctgtatcgat attctttcta tttttatggt ttgaatatcg
     9421 ttcactgaga tcaccgcttc atgaaaaaga atcatttgct tcagctgatg tatggcgtat
     9481 taattctggt tgtaatcatt acaacggcac agtcatgcaa taccttcaga acatggtttg
     9541 gtaccggagg ggacttcgac agaaagcacg tcagggctga acgcaaggcg ctgcgcgagc
     9601 agatggaaga agatgatctg tggggcggtg acgatgaata caacccgtgg gaagaaaacg
     9661 acgaatatga ccgggatcgt tggtaaaata ttttcttatg aaaactcttc gcaccataaa
     9721 acttctgctg ggggcaacac tgctaacact gattggtgct gtagttcaat cttgccggcc
     9781 ggtcatcaat acctgctata aaccggcgcc acccgacgac cgcgattcat ccgagtataa
     9841 aacaatccgc aattcgtgct acgatatgcc ggcagagccg atggacacaa caatgcagca
     9901 atgaaaaaga gcaaagtcat tttataccgg tcactgtatt ttacaggaaa aatattttcc
     9961 tggcagcgtc tgatgctgtt tgccggagct tcgttgctgg cggcttcgca ggcttgcaga
    10021 ccgactcaga aaatgtgcta tgaaatagcg gtgccggatt caacaacttc ttctaccgac
    10081 accctgatca tgaatgaccg cgattcgctc taccagatga ttgattgcta tgaagcgccc
    10141 aattatcccg attctgtcta taaagaacct gacaattagt gattgttcat attcgcgatt
    10201 atgaaaaaga tatttctggt ttcacttcat tttataggac gcaccgtcag ctggcgctgg
    10261 ctttcaatga tagcgggtgt ggcactgatg accgcatgca gtgggaagac agataaagcc
    10321 aaggtcagca ccgacaacgt aaaagattcc gaaagagtca catgctatga gccggttata
    10381 ctccaggatt ctgctgttcc cgacaccaac gtgcggaatc agggtgaggt cataactcag
    10441 gacagcgttc caatcaataa tattccggaa aagcatacct gttatgcctg ggttgagtct
    10501 gatttataac actgaatcat gaaaaaacac attagtcgga ttacatatgg atttgcacgg
    10561 attaccggct ggcgaaggct ggccttgatt tccggacttg catttctttc agcatgccat
    10621 acaaaatccg atcaatcaag agttagcaag gattcaatac cggactcagt gcgggttaaa
    10681 tgctatgagc cgatggagtt ggacaccgca tcaactcagg ccgctaagga ttcagccaat
    10741 aaagaagaaa tgccggcggt taaagattct tctgagctga aagtaccgct gcatccctgc
    10801 tatgttcctg taaaaccaaa agaataaaat gaaaaacagg ataaccagaa ttttcattcg
    10861 tatgctgttg agcattaaca gtgtgattcc tgcccgttca gcagtatcgg ttttcctgct
    10921 cggcattgca accatgctga tgacgaccac tacatcttgc gggccgcgtg tcagctgcta
    10981 tgaaccgtcg gtggattcaa ccggcaccgt tcagatggac acgacggtga aaaaggacag
    11041 catagttcaa tcctctgtga acaatcaagc gaacagcaac agcgttaccg actgctatga
    11101 tatcgccatt ccgattgacc aggtggtgac cgactcaaat acaaatgtga ataaaccgga
    11161 tgatccaatc aaaacctgct atgcacctgt tttaaaacct gaataatcca accgatgaaa
    11221 aaagctgcac tcagatttat ttattctttg acccgcatga ccggcttgcg cagtatggct
    11281 ttgcttgctg gaattgcgtt gctgactttt tcatgcagtg caaaaaaaga acagaaatcg
    11341 caaaattccg attcatcctc aagcgactct gtgtacgaca cctgttacaa atcagtgcaa
    11401 ccgatgaaca gtgaaaaaga ctcaataaat taggtgcgtg gtatgaaatc agtaaaacaa
    11461 tttaccggtc attttttatt gagggcaatc cggttgattc cgccaaagtc agctgctgcg
    11521 ggatttatga tcgggctggc cgcattgctt accacgagca ccacatcgtg tgagccgcag
    11581 gtgatgtgtt acgatcccgc gccacccgac agcattccgc aggatactgt ggattcgaca
    11641 agacaaacac ttccaactga tattttcgaa aacgccgatg aaaaaagata agacagcact
    11701 tccgcatatt tcgtttgaaa ccgaaactcg ctgcaacctg cagtgccgct attgctacaa
    11761 tatctggaag atgcccggtg gtgagtttcc cggcgaagtg acctatcgca aggcccgtcg
    11821 ggttctgaaa aaattgtatc gacgcgccaa agtagatcac ttcacattta caggcggcga
    11881 gccgcttctg ttcgatcgtc tggccgaact ggtgttgttt gtacggttga aaggaagtac
    11941 cgtgactttg atcagcaacg gcaatgcagg tactgaagct gattttaaga tgctgataaa
    12001 attgggtgtc ggactttttg agtttccttt tcacagcatt gatgctgaaa tacatgaccg
    12061 gatggccggt gtagaaggtg cgcatgaaaa atcgaaggca accattgaaa tggtgcaacg
    12121 cctcggaggc cgcgtagttg ctgtgattgt gctgaccagg cacaacatac cgacattgaa
    12181 ggaaacgctt ttaagattga aagaaatgaa agtggcttcc atcatgctca accgcttcaa
    12241 cattggcgga gccggtattc agtgggccga agacctgatg ccatctctgc aggaactgcg
    12301 cgatgctttt gcattggtgg agaaaacgat tgtggagcat aaaatatcac tcacctcaaa
    12361 cgtgtgcaca cccatctgtg tgctgaatcc gaaagattat ccgcacatcg gtttcggaca
    12421 ttgcagttcg gatccgaagc tgaaacccat tacgctcgat catcacggaa atatccgttt
    12481 atgcaatcac agtcctgtgt atgccggaaa cattatggag agcagctttg aagagatttt
    12541 ttccaatgat tacgtaaaaa gctgggatga gaaagagccg gatttctgca caggatgcgc
    12601 cctgtatgca gattgctttg ccggctgccg tgccgccgcc gagcagatgg gacggacgat
    12661 tcgcggcgcc gatccggtgc tggagatggt tggggcggag aagccggtgg cggagtgatg
    12721 agttatgagt tatgagtgtt gataggttta gaggtttata tgtttatatg tttatatgtt
    12781 taggggttta gaggttgata ggagccgaag gctgaaagaa agctgcgatg tacaaagagc
    12841 gctgacttgt catcacgtga aacgtgacaa gctgtttcac gtgaaggcgc tgcagatgtt
    12901 agtccgggac cccgcccgct gtcaccctga gtgtttgaag gaacgaccca aaaaatatca
    12961 taagattcaa atgtatcgaa ggggcagcgg ggtaaactcc gtctcgggga cacaaatcgt
    13021 gtgcagcaca attgagagat tgtcggcagg cgccggatac aagcaatgaa taaaaaaatt
    13081 aaattgaatg ttcaaaaaaa tatttttcac ctgcatcatt ttcctctgcg caattagctc
    13141 ctatgctgtg agtcagagtg aggactgcag cggtgggcct gataccatta cagtggtttt
    13201 tgaaaagcaa ttcagcaaca aggtggtcaa agtgcttcac aatgattcgc tgattctgaa
    13261 agagaaagct atcactctgc gaaagcacgg tctggaagta accggcgttg gaacacagat
    13321 ttttgtcaca ggcgaaaaag agaaaattgt tgttcgctgc ggcatctggt cgaagcggat
    13381 ctgaattaaa aatgataagc ccaatgtcta tatccatcgc gtatggggca ttggtttttt
    13441 gtgggttcgg tatttggaaa aaccgcgcgg ggtttagata aaacagtcgt ccattactct
    13501 cccggcatta catccgggtt tcggcgggtg tggaaaattg cggtgatcac aatgtcattt
    13561 gtttcagaaa taaaatacag caaaataaaa ggaaaatcat ccattagtgc gccgtgaaaa
    13621 ttttcgtatt tgatttcaaa agtgagcgga gatctctgaa ttgtctttat caatgattgc
    13681 gaatttgaga aaaagcacat tcccagtcct gatatgaaga cggggcttga tgatattctt
    13741 caacccgttt atccagctca tctttctgtt cctggttcaa atcgtaagtt gaaggaattt
    13801 ctttcacgcg cacaaaccgc agacttttca tcagttcaat gaaaaaccca actttgtcat
    13861 catttacttc aaccgaaaat tgtttcatgg ctgtgggcgc tgtttgtttt ctgtaaaata
    13921 aagatacaaa aatgattacg tgtaaaaata ctcattatta tgatgaaaaa tacaattgat
    13981 aatgagtaaa aatactcaca ttattgagta aaaagtaaat atcgttttaa tatgcagaaa
    14041 atcaattgct taaaaacttt cactttgtcg gttaaaacac aaaaaaaaga gaaggctcgc
    14101 gccttctctc caaaccacct atgaaaactc attcattact gaatgctgat tactcttgag
    14161 agcttttcct ctgcttcttt tttcgggatg gtcacatcga ggatgccgtt tttgtaagct
    14221 gcttcaatct tgctgctgtc ggccacatcg ggcagaataa agctgcgcga gaagctgcac
    14281 tggctgaatt cacggcgact gtagtttttg ccttcctcgt ttttttcttc ctttttttcg
    14341 ctgctaataa ccagcacgtc gtccttgacc tcgattttga aatcctcttt ctccatgccg
    14401 ggaacggcca gttcaatctg atatgatttg tcgttttcgg ccacattcac tttcggcatc
    14461 cagcgcgtag ctgaagatcg gaaagcgggt acatcattga aaaattcgtc tagcactgac
    14521 ggaaaaaagc tgttgttttt gatcattggt aacatagttt tgtcctccta ttattttttt
    14581 gttgtttttt ttgatttcga ttcgcccttg tcaaatcgga gaccaaacga atttactttt
    14641 cttgccacag aattaaatcg cacaaaaaca gtttgttagt aaatttgcgc cagtgacaaa
    14701 acgacatgtt ttgcaattcg gagactgtca aaaaggcagc agggctgtca atctgtgcga
    14761 aatacatacg tcgctgtgcg aagtagttta cagctgtgcg aagtttttta acttcgcaca
    14821 caatatatcg caccatatga ataccattac agagctcttc gatacatcgg tcaggcaatt
    14881 cggcaacaat ccgcttatct gggaaaaagc agacgggcgc tacgtttcaa caacttatac
    14941 cgaagcatca gttttagtga accacctggc cggaggcctt atgaacattg gtttacagcc
    15001 cgccgacaaa gtggttttgt acagcgaagg ccgcacgtgg tggctgcttt cggagctggc
    15061 tgtaattgcc tgcggtgctg tgagtgtgcc ggtgtcaacc cggatagaag aagccgatga
    15121 aatgttgttt cgcatgcagc acagcgaaag caagtttatt attatctcgg cgcgttttct
    15181 cgactttgtg agagccatta agaaagaaaa cggtcagaca cataaaatca tcattctcga
    15241 tttaccaaat aaagcgaata ttgacttgca gtccgatgaa ttcttactgg gcaacctgtt
    15301 ggaaatcggc tccaaatgcg agaaagatac gatagcaacc ttggctgaca ttgctgccaa
    15361 aaccagtccc gacgatgaag tcaatatctg ctatacatcg ggcaccacgg ctgatccgaa
    15421 aggcatttta ctgacgcaca aaaattacct caccaatgtg catcagtccg aggccatgtt
    15481 tgatgtgacg cccgatttca tcaccctgct catgcttccc tgggatcatt cgttcggaca
    15541 cacggtcggc ttatatactt taattctgaa aggcgcatcc atggcggcgg ttgatggtgg
    15601 caagtcggcg atggaagcat tgcgcaatgc acctatcaac attaaggaaa tacgtcctta
    15661 ttttttactg agtgtgccgg cgctggctaa aaattttaaa gccaatattg aaaaaggaat
    15721 taccgataaa ggcaaatttg cttctgtgct attcaccttt tttctgaaca caagcatccg
    15781 ctatcatgcc gatggataca acaaaggcaa aggcctcaga tttttattga aaccgttggt
    15841 ctggctgggc gatcaaattc ttttcagcaa ggtgcgtttg gcatttggcg ggcgattgcg
    15901 atattttgtc gggggcgggg cactgctcga tgctgacata cagcgctttt tcaatgccat
    15961 tggagttcct atgtatcagg gctatggcct gagtgaggcg tcgccggtga tttcatcaaa
    16021 ttgtcccaga ggatttaaaa tcggctcatc tggcaagccg gtgccgatga tggaaattat
    16081 cattgcagat gaagacagca agccgcttcc gacaggccaa tcgggcgaaa ttttgatccg
    16141 gggcggcaat gtgatgaaag gttattggaa aaacgaacag gcaacaaaag aaaccattgt
    16201 tgatggctgg ctgcataccg gtgatctggg ttatctcgac gatgatggtt atcttcatgt
    16261 caaaggccgt tttaaaagtc tgttgattgc caacgatggc gagaagtata gtcccgaatc
    16321 gctcgaagaa gccatggtgt atcattcaga cattatcagc cagatttatc ttcataataa
    16381 tcagaatccg tttacgacag ctttggtttt tttgaatttt gaaaagctca aacaaaaact
    16441 tgcagctgtc ggattgagta taggaaatga ggggcacaga actgggatat tggaattgat
    16501 caccaaagaa atcaatttac ttcggaccga tccggtcctg agcagacaat atccgtcgcg
    16561 ttggctgccc agtatttatt gtattattga agatgggttc aatgagaaaa atcatttact
    16621 caacagtacc ttaaaaattg tccggcccaa aatagaagcc acattcaaag ggttgtttga
    16681 tatgatgtat gggcaggagg gcaagaaaaa aactgatgat tacaacatgg caatattgga
    16741 aaaaactcaa cagtagtgct tttgaagcat tcaaatttgt atttttgagg tcaaattgta
    16801 atatgaaaaa atccgttatt ctttgcctga tactgggtat tcttgtgcaa acgctgattt
    16861 catgcggtca tcagaagagt caagccatag ataatgattt tgaagttgtg gcagatacaa
    16921 tgcagtcgaa gattacgcct aaagttcagg gcaccataac ggattatgaa aagttattta
    16981 ccgaaggaga atcaaagact ctggtaaatc gttgcaacga gtttgaggcg aaaaatgcaa
    17041 ttcctgtagg tgttttcact tgcgacaata ttggtgattt taataatttc acggagtatg
    17101 ccgatgccgt ttctaccaag tggaatggct gcaaagacaa tcaggggcta ttgtttgtga
    17161 tatctaacaa actgggcgaa atcagaatga tttcgtgtcc tgctactgaa aaccgtataa
    17221 cggataatga ttttgatttt gtaataaaca cggtaatttt tgatgctttc agaaaaagtc
    17281 agttttttga tggaattatc aatgcccttg attaccttga tgataaaata aaaaagtaaa
    17341 aatgccgacg agagtttttt tcaatgaaca gcccgaaccc tttgtaattc ttaattatgc
    17401 cgaggatttg agttcggttt gtatttcggt aagtcatgaa gatcatgctg atccggtgga
    17461 gcttttcagt accggctgcg ggaaggatat tgctgaagaa ttgcaatcca aagaaggttt
    17521 ttcggtgtat atggaaggat gtaaaaaaat aaatctgcgt tatcggcgtt ttcttttcaa
    17581 aaagaaatgg agagtcatgt acgaggatgt tgaattatca ggaattaaag tcttatgaaa
    17641 gcatttttgt ttcaggttgt atttctgctc agtgtattca tacagccatt gtcggctcag
    17701 aataacaaat tcaaatgtcc gtgggatgaa attgactgtc cgggtaaatg cggtcgtttt
    17761 ttcgatgccg atggtgatgg tttttgtgat tatggccgcg tatcgaaagt tgaaaaaaaa
    17821 gatacgattg ttcgtaaaga taccgttgca attaaaaaag aaaatgttgc tggcacaaat
    17881 gtaattcctg ccgaagaatc cagtaaagtc attgttgatg cggattctga tagtgttcag
    17941 cttgaatccg aagctgttcc tgaaattaaa aatgaaatta aaactccatc aaaggctcgt
    18001 tcctatagtt tgttgctgat tacgcttctt tgttttggat tttacggctt tacttctttt
    18061 ttaatggagt tgaaattgtt tcgtaaattt gttcatcgcc gtatatggaa catactttta
    18121 ttgctcacat ttctgactac ggcagttctt ggaattgtac ttgtggttca aatcaattat
    18181 cagttggatt cttcattaat ccgtgattat ctttactggc atgtacaatt tggtatagca
    18241 atgtctgtta tttctgttat tcatattttc tggcactgga aatatttcta ccgtattttt
    18301 tccaaaaaaa gtaaaagcga ttgcgttaat tgatgaaatg tttgtatccc atatattgtt
    18361 tattattctt gctttttatg atagcaggtt tgcaactgcg tgcacagaat ctggttccca
    18421 atggttcttt cgatgacaaa aaagggacaa ggccgaccat gaaaccatgg aaaaaactga
    18481 atacggtcga tttttttgtc aatgccgatg accgacggta taaagaagtt aatgcagcca
    18541 gaaacgaccg caattatatt ttgcgtaaac cacgttcggg acatgcttat gttggaatcc
    18601 gcgtttggcc taaatacaat gaatatcttc agattcaact tcgacgtccg ttaacggaag
    18661 ggaaggccta ttcgttcgaa atgtatgtaa caccttcacg atattcgaat tgttttttga
    18721 aaaaaatcgg ggttagtttt tatccaaacc gacctcctta tactactcgt gctggtcgcg
    18781 aagattttcc gcctcaggtc gaaacttatc gtccttttgg tgtgcgcgat actgccgagt
    18841 gggttcgcat caacggagtt tttattgcca aaggaggtga aaagattatg actatcggca
    18901 atttttcaac cactcctcgt gataaattca aacgaaaaaa actttctttc aaaaagcgcg
    18961 aagcttatta ttatatcgat gatattgccg tctatgagct cgatgaaaag ggaatgccca
    19021 ttctcgattc gattacctat gctcttgcgc agcaggattc tctcgatagt gattcggctg
    19081 attttatcgc atcgaccttt gcaggcctcg acagcaatat caccaatatt tattttgagg
    19141 aaaacagtgc taaactcgat gaggctggtt ataaaaaact tggtattatt actgaattcc
    19201 tttttgaaaa tccgggcgct actattcgca ttgagaccta tgcttcgcct ggagaaatgg
    19261 atggacaaga gatgaaattg tctgaaaaac gtgctaaatc agtttatata ttcctttccg
    19321 gcaataaaat tgaaaaagat agaattgtca tcagacctgt tggtaacagt tgtgaatttt
    19381 ataaggccgg cgaccccaaa cgacgttttt gccgaaaagc tgaaatcatt attttgcaat
    19441 aattcaaccc ttcaatatga aaaaaatact tgtcgttttt ctggctttgc tcattgccgg
    19501 ggcattgcag gctcaggagc catttaaaat ctcaaaggac attccacata ctccggtaaa
    19561 aaatcagcag catgccggta cctgctggag ttttgcaacc gtatctttta ttgagtctga
    19621 acttttgcga agtggtgcag gcgaatttga cctttccgag cttttttttg tgtattatgc
    19681 ctacctgatg aaaacggaaa attttatccg cttgagaggt ctggccaatt ttagtcaggg
    19741 tggacaggct catgatgtat ttgctgtgat ggataaatat ggcgcagtta ccgaagattt
    19801 tttcaatggt tatgcaccag gcgatacatt atacgatcat tctgaaatgg aaaaaatatt
    19861 aagtgcaatg gctaaagtct ggacaggaaa agatggcgct ggtcatcatt ggaaaagcgc
    19921 tgtcagagca attctcgatg tttatatggg tgtaatgccc acgcaaatga ctttcagaaa
    19981 aaagacctat actccaatgg aattccgtca ggcattcttg ccaaagtctg cggactatat
    20041 cgaactgacc agtttttcgc atcatccatg gtttcatccc ttccgtctcg aaattccgga
    20101 caattggatg ggtgctcaat actataatct tcccattgac gatctgatga gtgtaattga
    20161 ttatgcactc gaaaacggct atacagtttg ttgggatggc gatgtgagtg aaccggaatt
    20221 caagcatcgt cagaattatg cgcgcctcga aaatgaaaac ataaaacttt cgcaggatct
    20281 tcgtcagaag gatttcgata atttcagtac ttcggatgat catttgatgc acatgactgg
    20341 tctttcgcag gattcggccg gaaaccgcca ttacattata aaaaacagct ggggtgattc
    20401 caacagcgaa catggttttc tgcacatgag caaaggattt gtttatatga aaacggttgc
    20461 cattcttatt cacaaaaatg caatacctga aaaaatccgc acgaagcttg gaataaacta
    20521 agtcttgatt ttggacgatt tttttccaca atccggctca ccgaaacttt gtgaaatttc
    20581 ttttaaaaga cagggctcgt ttgttgcatg attgttaaaa acaatctcaa agctattatc
    20641 tgacgccgtt ttccgcttgt tgataatttt gcgtgataat attcaacttt catgactttg
    20701 tggagtcaaa gaaattcaca agcccggatt catgagagtt gtaatattaa tattttttct
    20761 gtttatagtt gggtctatga tggcgcaatc cgaaagtcag gttatgtctt tatcggattc
    20821 gcaaacgtat ggtgtagttt ctgacaaatc attaaaaaaa aatgttgatt ccatcatacc
    20881 tgcgcatatc attattgatc cggcgcaggg agttgcacct cttgctgtgc aaatcagcac
    20941 tccggctccg gctctgagtt gcacctggat cattgaagga atgactttca ctgatatttc
    21001 tgcattcagt catgtttttc aacatgcagg atcctatgat gtttgtctgt tcacattgaa
    21061 tcatggcgga tttaaagatt cggcctgttc cgtagttcag gtttgggctg gctcacagca
    21121 ggactcttct tttcttgtcg tgccaaatgt tttcacgccc aataatgatt tacaaaatga
    21181 ttttttccgc accaccagtc tgaatattgt tgaatgggac tcacgtatct ataatcgctg
    21241 ggggcagctg gtttataatt caactgcagc agatgtgatg tgggatggtt tgaataatgg
    21301 aactccgtgc gatgatggtg tttatgtttt catcataaaa gcagttggcg gtgatggaaa
    21361 attatatgat atttccggaa ccctgaccct gttccggtag ttttcagcaa ttaataaaac
    21421 ccagccggaa agaggtctgt attaacagat ctctttcttt ttttagaaca cagatatttt
    21481 tttatagacg gatttttttc agaatttttc caagtattat cgccattttt tttatttgat
    21541 tgctgttcat tccatagaaa agcggcaatc ttagcaaacg tatgttttcg ctggctgtca
    21601 ccgggcagtt gccgctaaat cttttaccaa acaagctggt atgcaatggt ggaaaatgaa
    21661 attgggcttg tattcctgca ttgttcagtg cgcggtgaat ttttgcggaa atgttctcgg
    21721 agtttccttt gatgaaaaaa atatgggcat tgaaatcgtc ttctttatct ggtaaagggg
    21781 gcaaagaaaa atatttagca aacccatcga tattacccaa tagttcgtaa taacttttcc
    21841 ataattcaag ccggcggtaa ttgacattat cggtttcctg caattgagcc agtagcatag
    21901 cggctgtcag gttgctcatc gaaaatgagg aacccgcttg tatccattcg taaaaaggaa
    21961 cttcgccccg aagaaacata gacctgtttg ttcctttctg aaaaacaatt tcagctgttt
    22021 caatcagatt tttgtcgtta acgagcaata ttccgccttc gccacaatga atgtttttcg
    22081 attcatgaaa gctgatacat cccaaatggc caattgagcc ttgtttatgg ccatcaatgc
    22141 cggcgccgat agaatgagct gcgtcttcaa tcagaaatat atttttactg tcgcatattt
    22201 ttttcagatg tattacatct gcactatagc caccataatg gacagccgca atagcttttg
    22261 ttttatcaga tattttactg ataacatcgt tgacatccag tgtcattttt cctgcttcaa
    22321 catcggcaaa aacaatgcgg gcaccacgca gggtaaaagc attggcagtg cttacgtgag
    22381 tccatgacgg aataatgact tcgtcgccgg cttgtatttt gcagatgagt gcagccattt
    22441 cgagtgcatc ggtacacgaa gtagttaaaa aaacggaagg cgttttatag tttattgcaa
    22501 accattcctt aacccggtcg ataaagattc cttcactgct gcattgtttc gactgcaggg
    22561 ctgttttgat gtaaaacagt tcctgtccgc tgatgtatgg tatattgaat ggaatgttca
    22621 atatttcaga gcgttttcga tgatcagatc gtataattct gtttcctgta ttcctgctgc
    22681 ctgtgcctgt tgtggtacaa tgctggccga cgacattcca ggaacagtgt tgacctcgag
    22741 aaaaactggt tcgtttccgt tgtgaataaa atcgaagcga accacacctc ggcaattcag
    22801 tttttcatat agcattttcg atgcagcaac cacctgatcc gtttttttct gatcaacagg
    22861 agccggtgta atctcatcgc tcattccctg ggtgtatttt gcctcgaagt cgaaaaaatc
    22921 ttttttactt ttcacaaggg taaccggcag ggctgtaatt tcgctattaa tgcgtaaaac
    22981 accacaggtg agttcatcgc ccattgtgta ttcctctacc aatacttctt tgtcttctct
    23041 gaaagctttt tccaatgcgc cttcaatttc ttcggcggtt ttcaccttac tcattccaat
    23101 gctggagcca ccactgtttg gctttacaaa aaccggaaaa ttcagatgtg taaaaacatc
    23161 ttcaggcctt gccgaagctt cattgttgaa atataccgat cggctggtgg tcaatccgag
    23221 tgctttaacc aattgaatgg tgtagaattt attgaaagta acattcgaaa ccatggcatt
    23281 gcaggtagta tgaggtatgc ccagcagttc aaaataactt tgaagcagcc cgttttcgcc
    23341 cggtgtacca tgaatcatga taaaggccac atcaaaatga attgtccggt ccggagtttg
    23401 aagcgaaaaa tcgtgacgat tgatgtcaaa ggtttcgttg ttgtttttcc agcaccaacg
    23461 ttctttatta atttctatca gatatacatt gtatttttcg cgattgattt tctgagcgat
    23521 aacgccggcg ctttgtacag aaatgaccga ttcgcccgaa tatcctcctg caagaagagc
    23581 aacattaagt ttcatatgtg attattataa atcaaaagta gcaatattca gtggcttggc
    23641 agcaaaaatt atttaaaaaa tagtgctgaa tctgaaaaat gtatctactt ttatatcaca
    23701 attgttgcga aacaaataat ggcaaaatac aataaatggg tttttggtgc tttgggatgg
    23761 gcttttattg gtcctatcgg aggtttattt ggatttgttt tgggagcata ttttgattat
    23821 gcatcgcagg aaaaattaca caccaatact gaaggagcaa caactccagg tgatttcaga
    23881 ctttctttgc tggctttatt ggctgcggtt atgaaggttg accgtaagaa tctgaaagtt
    23941 gaacttgatt tcattaaaca ggttttcaat aatacttttg gagctcaata cacaaaacac
    24001 tccatgcctt tgctcaaaga gttgctgaac aaagaaatac cggtcagaga tatttgtttt
    24061 caggtgcgca ctcatatgga tcatgctgcg cgtctcgaat tggtaaatgt gctgctttcg
    24121 ctgtgcatgg ttgatcagga attgcatccg gcagagaatg agctcatgaa gaagatttcc
    24181 atgtggttgg gaatcagcga caaggattat caatccatgc attctgttca tattcccgat
    24241 actggatggg catacaaaat tcttgaagta aaacccgatg ccagcgatga tgaagtgcgc
    24301 aaagcttatc ggcgtatggc catgaaatat catccggacc gggttgctca tctgggagat
    24361 gatgtggcaa aatcagccgg cgaaaaattc aaagctgtca gtaaagcatg ggatgaaatt
    24421 aaaaaggaac gtaatatcaa ttgaattttt taaaaaatca gagaaatatt tagtactttg
    24481 gcgtaaattt tcagaaacct ctctgcatgc ggaaaattgt gatgatatgc cttttgtttt
    24541 ttgctgttct gcctttggca aaggcgcagg aactcagggg aggtttgcct ttttttgtca
    24601 attatcatcc cagtgaatac cggggttatt ctcagaactg ggtattaaca agcaattcca
    24661 aaggtttttt atttgttggt aatggcgatg gtattttaat gtacaatggt cgcacctggt
    24721 cgaagttttt gctggccaac agtgcaactc ctgccagttt tttttgtgat gaaaatgatg
    24781 tgatttgggt tggtgcccaa tcagaacttg gatgtttttt gcccgacaat acagccggct
    24841 atcgttatgt ttcgttgact catctcgtac gcagtaaatt cagggaattt ggttttgtgt
    24901 tttccatcaa taaaacaccc gatggtattt atttcagaac ggcagaaaat atttttcgtc
    24961 ttgttcctca aggaatgtat gttatcccgg ttgaaagagg ttcgcattta tttgaattca
    25021 ggcatacttt gtttttatat gagccatcaa aagggttttc tgttgtcagt ggcaataaat
    25081 tgctgagggt tatcagcgag cttcctgaac agatggttat ccgcaaagta attactcttg
    25141 ggcgcgattc ggcgctgata gtcgattctc agcatggtct gcacaaggta tggtttgact
    25201 tcaccaaccg ctatgccatt aaaatgcttc ttaagccatt gtcggtgcca atcaatgatt
    25261 ttcttgttag caacgaagta tttcatgcaa tccgtatgac caacggaaat tatggttttg
    25321 caacaacgcg taatgggaca atcgtaacgg actcttgttt caatatttta tatcatctct
    25381 cgcgtgaagc aggagtaatg aatgagaccc atacttattt gtacgaagac cggcagcata
    25441 atatgtggat tgcaatggac aatggaatct gcaaggtcaa tgcgcagtcg ccattgtgca
    25501 attttgatga ttatcagggt gtgaaaggca gcgtgctcga tgttgtgcgt tgtttcaatc
    25561 agatatttat tgcaacgtgg cagggggtgt tttatcagaa tagggcagag gacaggtcta
    25621 aaaatgaaat attccttcct cttccgcaaa ttgcatctca gtcctgggat ttggagccga
    25681 ttgtagttgc aggcaaatgc tatttgctgg ccgctacatc ggatggtgtt tttctgattg
    25741 acagtacttt aaaaaccaca agggtgtctg atggcaattt taattatgtg aaagcacgga
    25801 gcgacaacaa tcagttggtt tatgccggtg gcccgcttat tatcaaatat ctcgatttca
    25861 gcaaaagcat tacaaagccc aaagaaacac aggtcccaaa tctcgaaagt cgtatcatca
    25921 atgcagtttt gtccgaaaat aatcagttat ttgttggtac tgcacgtgat ggtgtatttg
    25981 ttttttccgc tgtgccttta tccggctcgg gtgatgatat ggtttttgaa atgagtcaga
    26041 ttggagtcag aaatggttta cctgagtctg atcaatacag cgttttccgt tttcgcaatc
    26101 aaactctggt cagcgcacaa aatggtcttt accgcattgt tcagaaagac catcattatt
    26161 cggtgattcc ttttgaccgt gagtttttta ctttttataa taaatctcag tttattaata
    26221 ttctcaaaga agacgatgcc ggaaacatgt ggtttcagac taattcgaaa ctgaccggcc
    26281 ataaaagtct ttactatgtc aaatggagtg gcagtaaaaa agaattcatc agttctccat
    26341 ttatggcttt tcccgatctt gaattttatt ccataacgcc ggaaggagat tctgttgttt
    26401 ggcttggtag cgacgacggg gttttccgtt ttaattggaa agtcagtaat tcttttagct
    26461 ttgcagagcc atttaccaca tcgattcaaa aattttctct taatggagaa acgctttaca
    26521 gcagggttgc acgcaattat ctggtttcag aacccgatgt tgttgctttt aataaaggaa
    26581 atatccgttt cgatttttcg gctggttatt tcattcatga aggtcaggtg cagtttattt
    26641 atatgcttga aggatttgat gatcaatggt ctgagccatc ttctgaaaat tataaggaat
    26701 acaccaattt gcctccgggt gaatatacat ttcatgtaaa agccatcaat cctttcgggg
    26761 ctgaaggagt agctgctgat ttttcatttg tgattcctgc cccttggtac aacaattggt
    26821 gggcctgggt tgcagccgca tttctggctg ctttgctggt ggttggtata gttacttatt
    26881 tcaatcgtcg tcttataaaa gccaaactcc gccttgaaaa cattattcat agccgtacac
    26941 ttgaaattca aaatcagaaa aaggccatcg agattgaaaa ggaaaaggcc gataaattat
    27001 tgttgaatat tcttccggta aggatagccg atgagctgaa gtcaaccggc cgatgtcaga
    27061 ccgaatttta tcagtctgtt tctgtgcttt tcacagacat cagtggcttt acgtcgatat
    27121 cggaattaat ggatccggag gaactcgttt caaagctgga tgagattttt acccagttcg
    27181 atgatatatg ttcgagaaat aaacttgaaa aaattaaaac catcggtgat tcgcacatga
    27241 gcgcaggagg tattccggtt cgtaaccgca cgcatgctgt cgatgcaaca ttggcagcca
    27301 tggaaatgca gcagtttatg gaaaaaatgc gtcaacgcaa tccagccgac agcatatgga
    27361 aactgcgcat tggtattaat accggtgagc ttactgcggg agttgtaggg aaaagaaaat
    27421 tcgccttcga catctggggt gatacggtta atacggccag ccgattgcag gatgccggtg
    27481 agtccggtaa aattaatatt tcgaaccgaa ccgccgaatt ggtttcgtcg tttttcgatc
    27541 ttacttatcg cggcaaaaaa cccattaagc ataaaggaga ggtggatatg ttttttgtcg
    27601 atggcattaa ggccgaattg agtgttgatg gcaaaaagct tcgtccaaac aaagctttct
    27661 gggataaata caatgaatta cttgaactca ggtttttaaa tttctgacaa tggactttga
    27721 acgggtgaaa gattatatga tgcagcggct cagcagggga ctggcaccca atctctatta
    27781 tcatggaatt catcatacag ttgatgtgat gcgtgcagcc gaagaaattg ctgccgccga
    27841 aagggtggtt gggatggata aaacaattct gatgactgcg gctttgtttc acgatgtcgg
    27901 atttctgatc cgatacaagg aaaacgaaga tatttcggcc ggtatgtgtt ggaatatttt
    27961 gccgcgcttc aattacactc aatccgaaat catgatgatt gcccatatta tcatgtcaac
    28021 ttccattccg caaaaaccag ttgataaact ggatagcatt atgtgtgatg ctgatctgga
    28081 ctatctgggg cgggatgact tttttattat tgggtgtaac ctgagacgtg aatggaaaga
    28141 atatggccgt gaacttactg ttaaagaatg gtatttccag caaatcgact ttctttccgc
    28201 tcatcattat tttaccaaaa cggcaacatt gcttcgtgaa gaaaaaaaat tgcgccattt
    28261 ggattattta atccggttgg tcaataaaaa gtagtatttg tcgttccttt ttatatcttt
    28321 gcaaaaaaca gaataatcat ttaactcaca aataatatgg aatcgagaaa caaaatcgtt
    28381 attgttcctt ttgattttac cgatgttgcc gaatgtgctg tgaatcacgc tgctggtttg
    28441 gccaaacatc agggctggac tgttcagttg ttgcatatca ttaatcgcga cacccgtgct
    28501 ctccttaatg tctataaagt gaccgatgac attgtgaaag aacgtcttca ggaaaaagtg
    28561 gatgagtttt caaacaaata cggtgtgcct ttctctttcg atgccaaaga aggcagtatt
    28621 tttaccgata ttggcaaaac agccgaagat gtcggggcta atttcattgt gatgggcaca
    28681 catgggaaac agggaattca acatattttt ggctcatatg cacataaggt gatcacttct
    28741 acccgtatcc cttttattat tgttcagaaa cgcgatttca gagaaggtta caaccgcatt
    28801 ttaattccca tcgatgatac attggagtca cgtcagaaaa ttaaatgggc catctatatt
    28861 gccaagcagt tcgatgcaac catttatgta aaggccatgt atctcaacga tgaattatat
    28921 aaagcaaaga tccgcgctgt ccttaataaa gttgttgaag tgtttgaaaa gaatgaggtg
    28981 aaatatgaag ccgaaatcat tcccgaaacc ggcagctttg ctaaacaggt gattgattat
    29041 gcccgtaaga ttgatgctga tatgcttctc gttatgacca ataccgattc aagcattgtt
    29101 cccaatttca ttctcaatcc atgggatgaa cagattattt tcaacgatgc gctcattccg
    29161 gttatggcta tcaacccacg tgaaatcaat atcactgtgg tgggacttta atcctaataa
    29221 ttttttcagg ccggttgaat ccaatactca accggctttt tcatatctga aacgtcaact
    29281 ttatgaaccc aaaaaattta ttttcggaaa cagacatcag gcagattgaa gaacatggct
    29341 tgaccgtttt gcaggtcgaa aatcagttag ctggctttag gcgagggttc tcgcctgtca
    29401 aacttattgc cgctgctaca aacaacaatg gaattgtttc atttagtcag aatgaacaaa
    29461 agaaacttat tgctttgttt gaggaaaatg cttctgatta ttttatggcc cgttttgttc
    29521 cggcttcagg ggctgctacc cgtatgttta aagagatctt tgctgctgtc gatgcattga
    29581 ataatcattg ggatatgccg atgcccgctg aaaaatttct tgaaaatctt aaacgatttg
    29641 ccttttatga tgcattgaag gatgttttga aaaaacatgg gcatgatata gactcattaa
    29701 ttgaaaattc agtctggttg ccggttttag atgccgtttt aaaaaaagat ggattgaatt
    29761 tcgcctcaaa gccaaaggca ttcattcctt ttcatcaata caaagggttt acccgaacag
    29821 cgcttgaaga gcagatttat gaaggtttga cgcacgctgt agggcatcat aataccatga
    29881 atattcattt caccatcagc ccggaacacc tgaaggaatt taaaaaacaa acacagaagt
    29941 gtcttgcaga actgaaagcg aagtgtgata tcaatattaa aatttcgcat tctttccagc
    30001 aaaaaagcac cgatactgtt gcggttgata tggataatat tcctgtgcgt gatagcaacg
    30061 aaagactggt tttcagacct ggtggtcatg gtgctttgtt gcagaacctg aatgacatta
    30121 aggccgagat ggtgtttttg aaaaatatcg ataacattgt tcctgattgg catagaaatg
    30181 atacggtctg gtttaagaaa gtgcttgccg gctatttgat gcaggtggtt aatgatattc
    30241 atgcttacct gattatgctt gataatggca atgttggcaa tgaggatttg aatgaaatga
    30301 tagtttttgc atcggataaa ctctttatcg aaattcccga ttggcttacc gaagctgatc
    30361 cattggaaaa aattgatttt ttgtttggtg tattcaaccg tccggtgcgg gtttgcggca
    30421 tggtgaagaa tgaaggcgaa cctggcggag gtcctttttt tgttgaagat tcaaatggga
    30481 atatttcatt acagatcgtt gaatcttcac aggtcgatat ttcaaatgct gatcagaaaa
    30541 aaattctttt gtcttcgact catttcaatc cggtggatct tgtttgctgg aaaaatgatt
    30601 tcaatggcag gccttttaat ctgaatgatt tttctgatcc ggaaacaggt tttattgcaa
    30661 agaaaagctt ccaaggaaaa gacatcaaag ccatggagct tccaggattg tggaatggtg
    30721 ccatggccga ttggatcaca atatttgtcg aagtcccttt gagtaccttc aatccggtga
    30781 aagaaatcaa tgaccttttg cgcgagatgc atcagtaggt tcagctgtca gctttcagct
    30841 ttcagcggaa atgaggtcaa gcgtcaacgt aaaacgctgc aagcaggaga cgcgcgctca
    30901 ggtcagattt aggttgagag gttgatgagt taataggttt aggtggcgac tgtgtcacgt
    30961 tttacgtgaa gccgtcaaaa agcgacgaaa tacaaatagc gccaacggcg ctgcagatgt
    31021 tagcccgggg cagagtcccg ggtccaaaag aaggatgcgt agcacaaatg atggtgttat
    31081 cgaaagtttt tgatttatat caatcaatgt ccgcttaata gtagaaagct aattcgtata
    31141 tttgcattaa tgcctaaaat agcatcaata atctttctat tgctcgtatc cgcaatatcc
    31201 aatgcgcaga caaattcttt ggaatttcac aacaggattg ttgcgcagct atcaaggatt
    31261 gacattgctg taagccgctt caacgaaggg attgtgatgt atgacggcga taccattctc
    31321 gctagataca aaaatttgct atcggaaaca gaagcatcaa ttgctgaaac agaaaaaata
    31381 tccattgcat cgggtgggga agaatatcac aaagccgcat tcgaactttt ccgtttttat
    31441 ctgcgtttat tcgaaaatga ttattacgat gcggtcgcca tcatgtatcg cattccgatt
    31501 accgatgccg atatcgaaga aatggagcgg cttttcgatc gcatcgagct aaaggaatcc
    31561 atttttctga atgcattttt taatgcacag attgatttct gcgaagctgc gggaattgaa
    31621 attgagtaat tgcaaatttt accaaaggga aatatataat ggcccatatt ttggatgact
    31681 ccggaggagt cacatgtctg tagaaaacaa ttcaaaaatt gacgactccg gaggagtcga
    31741 atattaaaag cttcgacatc tgaaatgctt gtctatatgc gacccgccag ctggcgggtc
    31801 gtgttgtttt attggttttt ctacagatat gtgatgcctc tggcatcagc cggaatattt
    31861 ttaaacaacc atgttggact atattatatt ttacaatggt attattgttt cacaatccgc
    31921 gcaatgcccg ttgaatctcc gctctgaagc tttagtagat acatgccatg ttcgagcgaa
    31981 gaaacatcaa tcccgctttc agaatattga ccgctaagca gcaattttcc ctgcatatca
    32041 tatatctcaa attgagaatc attgccggca gtaatatttt caatcataat gatattttca
    32101 gcaggattgg gataaacact gagttgcagc tgtggtgctt cgggtaccga aacatgctgc
    32161 acctcgatga agctgagcca cacatcatat aatccgtgat aatccgcaga caaatcggtg
    32221 gagttggaat tggtgcacaa tacatattga ttggtgccgg tttttaaggc cctgacatag
    32281 tattcaacat ctgaattcat cccgtcaata ttgtttcctc cgagtaaata aggcgttatc
    32341 gatgttaatc cattaaaatc gactgcacaa acttccagac ttccatgtgt tggggtagtg
    32401 cagttgagta atgaatcaga aattgttgtt ccgaaagcca tggcctgatt cgggccttca
    32461 tatattacat ctgaaaaaaa caaatctttg ttgtccaacg tattttgaga gccacatgat
    32521 acaaaatctc cattttcatc aataacgtaa taaaatccgt aataggccag tactttatcg
    32581 cgaaacatga aatcaccatc attactgttt gaatatccaa tcatgacaaa agtggaatcg
    32641 ttcagatact ttgcatcgac aataagatcg gtcgatgaac cgccgtatgt ctttgtccac
    32701 atagtatcgc ccagagaatt cagtttcatt acaaaaacgt cattgttgcc gtttgcgtca
    32761 tggacatcgt gattggttga agcagaggtg ccgaaaacca tgatgttgcc gtcaccgagt
    32821 tggtatgccg caattgcacg gtcgtcgtcg gtgccgccgt aatgccggct ccatgcaacg
    32881 gagagattgg attgcagact cataacccag atgtcgctca taccataatg accactcagg
    32941 tttccatcgg atgaaaatgt gtagcctgaa acaatatatc caccagagat tttcggagag
    33001 attttcgccc cgaaatcatg caaagtgcct ccaaaagcgg ccccgctact caatcctaca
    33061 gctgggtcgt atcctttgat ccatgtgtca ataccgccat gtcctcccgc aaatccgtta
    33121 tcgtcggaat tgctgttgat gagaaacacg taatccggac tgctgaattc cattacatca
    33181 gcaatttcat cattagccaa tccgccaaaa gcaacggagt gtaatgaatc gccattggcg
    33241 tcaactttca taaaccaggc atcttccgca ccgttaatct gtggaatgtc gccacccgct
    33301 gcatttgaat atccacacca gaaaaaacca ccatccgatg cctgataaaa ccgtgtaata
    33361 ttgtcaattc ccggccctcc gtaattgcgg ttcagtgttg tgtttatctg agctgaaagc
    33421 gacgtggaaa tgattaaaag aaaaactgcc aggatttttg ttttcatggt atttcggatt
    33481 tttaatctgc aaaaataaca tgaaaatgta aatattacaa ataaaagaat gttgatgttt
    33541 attaatctat agttttgctt aatacagcga aatgaaatat aaaagtcctc tggtttttac
    33601 aaaggcattg aaaaaaacta taacttggct gcagatttat gaattacata tatttcatta
    33661 tatcactgag ggtaaaggcc agctttgtgt ttctcaacat caagcggcga gatgattttt
    33721 aatagattgt ttcttcatta atagcttgga gtttcttcag ccggagatat tgttgtagaa
    33781 aactctattt tcaaattttc aaatcatcaa attttcaaat caaaagttat gaacttacca
    33841 tacgcagaac cgtataaaat caaaatggtt gaacccattc acatcagcac ccgcgaagaa
    33901 cgcgaaaaat ggattgccga cgcccatttc aatttgttca atctcaaaag cgaacaggtc
    33961 tttgttgatc tgctcactga tagcggaacc ggcgccatga gcgaccgaca gtggggtgcc
    34021 attatgaccg gcgacgaaag ctatgccggc agccgttcct attatcgttt gaaggatacc
    34081 attcacgaaa ttctcggatt tccttatttc atgccgactc accagggccg tgccgctgaa
    34141 aatgttttgt tttctgtact cgtaaaagaa ggtgatgttg ttcccggtaa ttcgcatttc
    34201 gatactacca aaggacatat tgaattccgt aaagctcacg ctatcgattg caccatcgat
    34261 gaagctttcg ataccgaaaa cctgcatcct ttcaaaggaa atgttgacat caataagctg
    34321 aagaaagttt tcgaaactta tccgcacgat aaaattcctt tcattattgt tactgttaca
    34381 aacaacaccg ccggcgggca accggtgagt cttgagaacc tgcgcgcagt aaaggaattt
    34441 gcaaaaagtg taaataagcc ggtattgttc gacagtgccc gttatgccga aaacgcatac
    34501 ttcatcaagg ttcgcgaaaa tgcctgtgcc agcatgagca tccgcgagat tgtacgcgaa
    34561 atgtacaaat gcgccgacat gatgaccatg agttcgaaga aagatgccat cgtaaacatc
    34621 ggtggattta ttgccatgaa cagcgaagaa ctctggcaga aagtaagcgt gttcaacatc
    34681 atgttcgaag ggtatgtgac ttatggcgga atggctggtc gcgacatgga agcattggca
    34741 gtgggtctcg aagaaggaac tgattttgaa tacctgaaaa gccggatttc acaggttgaa
    34801 tatctgggaa atcagttgat tgaatatgga attcctgttc agaagccgat tggggggcat
    34861 gctgtgttta tcgatgcctt gaaattttta ccaggcctgc cgcgcgaaca atatgtggca
    34921 caaacactgg ctattgaatt gtatcttgaa gcgggtgtgc gtggagtaga gataggaaca
    34981 ctactggccg accgcgatcc tgaaacacgt atggaccgtt ttccggcact cgagctggta
    35041 cgccttgcca ttcctcgtcg cacttattcc aacaatcaca tagatgtggt ggctgcagcc
    35101 ctgaaaaaca tctgggatcg ccgaagcact atcaccaaag gattgaaaat cgattacgaa
    35161 gcacccatca tgcgtcattt tacagttaag atgagcaggg tataattgca tactaatcca
    35221 taaaagccgc tgacgaataa cagtttgcgg ctttttttat tgtcgtttta ctgtaattac
    35281 ttcgttatta attccgtata tttgttaccc tttgtccgaa aagtttgttc aaccgttttg
    35341 atagcttttt ttatgaaaaa acgacttttt cttttttctt tcttcttttt tcttctattt
    35401 tttttaaaag cgcaaaccga cacgcttact gtgatgcagt acaatctgct caactacaat
    35461 aattacacga gttattgcac ttctgtaaac aacaatgtgt caaataaaga tggatactta
    35521 aaaaccattg ttgattatgt attgcctgat atttttactg tttgtgaatt gaatcagttg
    35581 cagacttcgg ttgaccgtat tttgactaat gtgatgaata ccagtggaag aatcaattat
    35641 gccaaaggtg ccagaaccaa ttactcctcg tcagatttga ttaatatggt ttattataat
    35701 actgacaaac tggttatgca tagttcaacc gctttgccaa ctgatatcag ggatattaat
    35761 ctgtacactt tttattataa agatcccaat ctggctgtta cacacgacac agcctgggtt
    35821 acgtgtatca tcatgcacct gaaagcgggt aattatactg gtgaacctga agaaagactg
    35881 gccgaagtta ccatactgat ggattatctg gatgctgtaa acaagccggg aaactatctt
    35941 gcaatgggcg atttcaatac gtacacgtcc gatgaagaat gttatcagtt gttgcttaat
    36001 cactttaata ccaatatacg tttttacgat ccaataagca aacccggcga ctggagcaac
    36061 aattgctcat ttgcatcata tcacacacaa tcaacacaca atgattttga taatgattgt
    36121 tttgcagcag gcggactcga tgatcgtttt gatcacatca tgacttctct tgatgtaatg
    36181 tcgggtggaa aaaatgtgaa atacatcagt ggctcataca atgctcttgg aaatgatgga
    36241 gctcattgta atgactcctt gaatgaagcg tctaacacat cagttcctgc aaatgttttg
    36301 gcagcactta tgggtaacag cgaccatttg ccggtaattc tggaactgca gattgatgtg
    36361 gaaacaggca tccacgatga agcaaaagtt ccttttgatt tctacattcc atccaatatt
    36421 gccgatgact atgggattct ggtatcgcct tgcagcggcc gctacagtat tgaagcaatg
    36481 aatgtaaccg gaagtctcat ctgtcgtttt caatccgatg ctggagttgg atttaataca
    36541 atcaatttat cagctgttcc aaaaaccgca tccatagcct ttttccgtat caccgacccc
    36601 agcggaaacg tgaaagtgta ccggacactg agatagtatt cagtggccag tattcagtat
    36661 tcagtagcca gtattcaata gccgagtagt cggtattcaa tgataagtta cgagtgatcg
    36721 gatatattaa aaatcgcaac tgactatcca caactgacca ctattactta ttttgccttt
    36781 ttattgagta gcatctcaat cgctttattg atgcggtttt cgcgcgtgtc tgattttttt
    36841 gccgaaataa tccattccac atattcgcgg cgatgagtga acgacatctt ctcaaaaatt
    36901 cctttggttg tttcgttttt atttaatgca gcttcaaaat cagcgggtac ttcgaccact
    36961 cgttcttcga tgtcttcaat aatgagtaca tccacagtat cgccgggacc ttttgcgatg
    37021 gcatcgcgca cttttttatt gagcccgatg atatgacacg gatgccccat ttttaccaat
    37081 gatccacgat attcgaagcc atcaaaatac gctttcactt ttatctgacc tttgcgtccg
    37141 aattcttttt cagcatcatg tggaaattca atgtagcccg aattaacgtt cggatttttg
    37201 atgatttttg cagtgaagcg gtattctttc atattttgga tttcatgtat aaatgtataa
    37261 tgttttgaac agaaaaaacg caaaacgatt atgaattttc tcaaaaatcc ccgtgttttc
    37321 gaaatgcaat ccaggatgca aatccgaata aacaatattt taataaaaag attgaagcgt
    37381 cttccgcttt cagcggaata cattccacgc ttataacgcg taacgaaagc caatcaaaaa
    37441 tctcacatcc cttcaaaaat caacaatcaa aaatcctctt gttccgttga taacacggaa
    37501 tgttcttaaa ttcacacttc cttgttcgaa gccttcaaaa atcaacaatc aaaaatcctt
    37561 gttcttaaat tcacacttcc ttgttcgata ttcattattc atatggtcca ctcatcatgc
    37621 acaaccccca ccagcacagc tttgccttcg cccacgcgga aaaccagtcg cagcgctttc
    37681 cagtccattc cgtcgtatct cggatcttcg cccttgatga aaaattcaac ccatttgcaa
    37741 tcaggatatg cctcgcggat attgttgatg gtgttgcccc tttgtaaaaa ttcattgatt
    37801 gaaatttcgc cttttttgag aaaatctgca tctataacaa agtctgcaaa atattgttca
    37861 atggtcattt ttatcggttc tccggagcca tcgtattctc cccatagaag cttttcgctt
    37921 gatgtagcaa tattgcgaat ctgcggcgca ctcatcacaa cgtctttgct tgtatcaatg
    37981 aatgcatagg ctgaaaagcg cagcccttgc ggtccgacgt attctgccag ctgactgaat
    38041 tctttgtttt tgaaatgttg cacaatcatt ttggcatcct gaataaggtc ttcatctgaa
    38101 acagaatctt tcaacgatgc agctgtacta tcagtcttca gctcctgatc ggaattattt
    38161 tttgatgaat ggttgcaggc aactgcaaat actgaaacga caaaaatcag aataaaaata
    38221 tttttcatgt tgctggaatt tatggtggca aatgtaatga aataatcgat atggtggagc
    38281 tgtggttatg ctgtcatcct gagacccgcg tgcagtcatg ctgacattat gcgacaagca
    38341 ccatcaaaac gcgtttgcaa cctatcacga tagctatcgt gaaaaaaatg acaaaaaagc
    38401 tgctgtcaca ctgagccccg ccgctgtcat gctgagccct gcctgctgtc actctgagcc
    38461 ctgcgtcccg caggataaac ttcgtcgaag agtcaacagc aggataaact ccgtcgaagc
    38521 acgaacagcg gggtaaactc cgtcgaagcg tcggcagtga aaatcaacaa aagaaaagtc
    38581 ttttgtataa gtggagatcc cggttttgtc agcaaagcta ttttgaatct gtgaccggga
    38641 acttaaatag gtcttttgtt gattttcaat ttttgtatca acttgatttg aacccttgaa
    38701 tttccgcacc aaatcataaa ttcaccatta ttgaattttt aatgtgtatc tttgcacaaa
    38761 ataatacaga gatactgttt tctttacatg gttatgttat agagttcaag tgcctcatca
    38821 aaaaatcttt catgacattt gacgatttct cactgataga gcccatcaag agcgctcttg
    38881 caaccgaagg ttacaccact cccactccca tccaatccga agcaattccc gtcgcactca
    38941 aaggcagaga cattctcggc tgcgcacaaa ccggcaccgg aaaaacagcg gcatttgcga
    39001 ttccaatttt acagctgctc aatcagcagc accagagcgg ggttgaccga aagaccataa
    39061 aagttctgat tgttacacca acccgcgagc tggccattca gatcggcgat agttttactg
    39121 cgtatggccg caatctcaat atcaagcaca ctgttatttt cggcggtgtt ccacagaatg
    39181 cgcaggtaaa cgctttgcag cgcggcgtcg aaattctcat agccactcct ggtcgtttgc
    39241 tcgatctgat gaaccagaaa tatatcacac taaaaaacat caataccctc gtgctcgatg
    39301 aagccgatcg catgctcgat atgggtttta ttcacgatgt gaaaaaaatt ctggcagcgg
    39361 ttccgcaaaa acggcagacg ctgtttttct cagcgaccat gccgcccgaa attgtaaaat
    39421 tgtcttcatc gattctgaat cagccggtaa aaatcgaggt cacgcctcag tcttcgacgg
    39481 ttgacatcat cgagcaatca gtgtattttg tcgcgaaggt tgataagcct tcattgttga
    39541 tgcatattct gaaagacaat tctcttgaaa cagtgttggt tttcacgcga accaaacatg
    39601 gagccgacaa agttgtacgt attctgaaca aaagcaacat caaggccgaa gccattcatg
    39661 gcaataaaac acagaacaat cgtcagcgcg cactgaataa tttcaagagc aaggtcattc
    39721 gtgttctggt ggcatcggat attgcagcac gcggaattga tattgaagag ttgtcgtgtg
    39781 tcattaacct cgataccccg aatattccgg agacctatgt acatcgcatc gggcgcacag
    39841 gccgcgcggg tgctgagggc attgccatct cattttgcga tgtcgatgaa aaaccttttt
    39901 taaaggacat tgagaaattg attggaaaga aaataccggt tgtgaccaat cagccttatc
    39961 cgcttgatcc gaatgcaaag attgagcgaa ctgagcgtcc gcagcgtcct gagcgcaatc
    40021 agcggtcgga cagaaggccg cagcaggaga agaaaaagca gtcatcgcat gaaacgagac
    40081 catccagaga acagggacag aagcgccctg agggatttcg caaacaaagc gataatcaca
    40141 gtggacagcg cgttcttgtg aaacgataat tgatatccgt tgaaagacaa cgttagataa
    40201 gttgtcaaaa attcgtactt ctaaaatctt atcgcgacat cgatctgatc cgccgctaca
    40261 gattccttgg tcatgattca atgttcagat gtttgtattt ctttcttctt tccttgttcg
    40321 ttgttcatga ttctttattc acttcttcct tccttgttcc tcttgatccg tcagaatgca
    40381 cggattgttc atgattcttt atcccgcccc tgaagccaac gatagcttag aatgccgata
    40441 attgtgagga taatcacgag tatcagatat gcaatccgcc agttttcagg accagtcatc
    40501 atagaatatt ccgacattcc tccgatgcct gcgataagcg tcaaaggcat aaaaatggtt
    40561 gtgatgaacg taagacggcg aatagtttta ttcgtgtttt ccgaagctat agccatttga
    40621 ttattagcca tggcaagatg aagttcgatg aggtttttta cagtttcgcg gcaggactcg
    40681 gtgagttcat atagccgaac cacatggtca tatacatcgc gatacaagta ggcagcttta
    40741 ttatcaataa aatcgtggtc atcgtgaatg atcctgttca gaatttcccg ttcatgaaac
    40801 agacttttgc gcaataattg aaaggcatat cggagtttct gaattttcgc aaaatcaatg
    40861 tcgtcagtcc ttgacaaaag gagatcttcg attgaatccg tttcatcttc aagtgcttca
    40921 agtgcatcca ttttttcatc cacgatttta tcgatgatgt aatgcagcag ccatccaggc
    40981 ccgttgcgcc atagcccgtt tttcgattca gacatttttt tcgtcagtgt tataatcgat
    41041 tcattgagcc tttcagagcg cgtaacggta atgataaatt tatctccgat aaaaagattc
    41101 acttcctcag catgaatatc ttcgttgtga tagttgaatg tattgaagat gagatgtgtg
    41161 tttttcggga acgattccat tttcggaatc tggtcatcgt ccatgcaatc ttcgatggcg
    41221 agcggatgca gaccatattc tccgggcagc tttttcagtt cttcttcagt tggctgaaag
    41281 aaatgatgcc agatgtacaa gctgtcggaa tcaagttccg gagctgccgg aagcacatcg
    41341 gctttcccgg ttgacgaaac atgcattgca aatgaagagc tcatgggtgg aaaattttat
    41401 taaaggtaaa aaatccgtgg aaattgaatt tggagttttt attttgctgt tcacgttatt
    41461 ataatccata aggattcttt ctggtccgcg gattacacag attgcactga attagtttca
    41521 aatttctcag aggccccagc tcggttgcac tgttgtcaac aattattgct tagtaataat
    41581 tattgactgg gttagtgtat aaatctatct gtggaaatgg cttcagtcgc aggctgaagc
    41641 catttcatcc gtttaaaatc caaagaccag cagaggtttt tgaaacagtg aaatccgtgt
    41701 aatcagtgga caaaaaattg actaacctga atcggtcttc accgcaggtg atggctgatt
    41761 ctattagcgt gcataagact ttacgggggg aagaagaatc ttcgcgtaga ttcgagtaat
    41821 tcgcggaaga aaaatcggag aagtgaccag aatgccaatg tgcctcatga attattttcc
    41881 aaagacctaa attttgtgga tattttgtaa ttccaacatc atttaacaaa ataaatttgc
    41941 ataccattaa taaatgtatt taatttgcaa ctttaattaa gctattaaac atgtcagtta
    42001 acaacgaaca aaacatggag aaaaccatta tggaggcggc cgagaagctg tttttagaga
    42061 aaggttttgc catggtttcc accacacaaa ttgccaaggc ggccggctgt aaccaggcct
    42121 tggtgcatta ttatttcagg acaaaagacc gattgtttga agccattttt gctgaaaagc
    42181 ttaaaacgtt tttttcggtc atttttcagc cacaggatga atcattgacc tttgaagaga
    42241 agctgaagaa atatattagt gcccatttcg acatgttgtt ggccaatccg aagcttcctt
    42301 ttcttgttat taatgagttg accacgaatg ctgatcgcat tgattcgctg aaagagatgt
    42361 tgggacaaag catcacttat gtgtatggtc cgtttcagca ggagttggat gctgaaattg
    42421 cggctggacg tatcagaccg acaaccattc ccgatatcat ttattcaatg gtttcactta
    42481 atgtaatggc ttttatcgga gctccgatta tgaaaaaggt tctttttctc gatgaaaaac
    42541 attttaaagc catgctttta taccgcaaag aagaaaacat ccgcataata ttaactggtt
    42601 taaaaccgga tgttgaaatt aaatctgaga agaaaacaaa aaaacgaaaa taaccatgtc
    42661 aacacgactt ttatttctgc tgttgttgtg tacggtgctt tcgtacacat cgcatgcaca
    42721 gctgactccg gagcagtgct acgaaaaagc acgcgccaat tatccacttg tgaatcaata
    42781 cgggctgatt gaaaaatctg aatcgtataa tctttcaaat gcggccagag cgtggttgcc
    42841 tcaggtttcg ctttcggcca aagccacatg gcagtccgaa gttattgaac tcccatttac
    42901 catgcctggt ataggagaaa tggatcagga tcagtatcag gcaacccttg aggtgagtca
    42961 gattttgtgg gatggtggtt caacaagcgc tcgcagcgcg atgtataagg cctctgctga
    43021 tatcgaaaag caaaagtatg aagtcgatat gtatgcgctt caggaaagaa tcaatcaatt
    43081 gtatttcggc attcttttat tgaatgagca attgaacctg aatgcagttt tacaaaagga
    43141 tctcgaaaca aattacaatc gtatcatggt gtcgctgaac aacggtgttg catcgcaggc
    43201 cgatctcgat gccattcggg tagaacagtt gcgggccaga caaatgcagg cagaattaca
    43261 ggcatcgaaa aaagcattta cagatatgtt atcggcattt atcggcgaac agatttcgga
    43321 tggaaccgtt ctttcaaaac ctgttgcagg tctcaatgcg tctgatattt acgaaatccg
    43381 gcgtcctgaa ttgcagttgt tcgaagcaca acgtaccttt tataatagtc agcagaagtt
    43441 ggttcgttca gtttcgatgc caaaattagc tttatttgca cagggcggat atggtaaacc
    43501 cgggctgaat atgctgagca atgaatttaa tccgtttttt atcggtggaa ttcgtttgag
    43561 ttggaatctg agcagttttt acacacagag caatgagatg cagctcctga aaataaatca
    43621 atccggagct gatgtacaaa aacagacatt tttattcaac accaatctga aagtttcgca
    43681 gattaatact gaaattgaaa aattcacgga gttgttgaaa tcggacgatg aaatcataag
    43741 actacggcag ggaattcagc tcagtacgga agtaaaagtt gaaaacggaa cagcatcggc
    43801 atcagatctg attcgtgatt tgaatgcggt taatcgttcg atgcttgaca aagccctgca
    43861 tgaaatacaa atgttgcagg ccatttacaa tttaaaaaac tcgacaaacc tataaatctt
    43921 tttcagatga aagccatcca ttatttactt gctttggtaa tgatcatttc aatcacttcg
    43981 tgtggaaaca gcaatggaaa cactgatgcg accggtgttt tcgaagcaac cgaaatcatt
    44041 gtatcatccg aagccaacgg aaaaattctc gagctcaatg tcaacgaagg cgatgagttg
    44101 aagaaaaatc agttgattgc cgtgattgac agcacgcaat tgtatttgaa aaaacttcag
    44161 ctcgaggcca gcgcaaaagg tgtcagaagc cgtaatccga atgtgcgcgt tcaaattgcc
    44221 gccacgcaac agcaactcga aacagccaga tcggagaaga aacggatcga aaatctcgtg
    44281 aatgcaaatg ctataaacag caagcaactc gatgatatca atgctcagat tctgtttctc
    44341 gaaaagcaat tggccgcgca gaaagaaaca ctggaaaata ccagttctgg tattggaagt
    44401 gaatcagaag caattaatat tcagattgcg cagattgccg accagttgca taaatgtcgt
    44461 attctcagtc cgattgatgg tactgtgctt gtgaaatatg ccgagcagag cgagtttgca
    44521 gttacaggca aagccatatt gaaaatggct gacacggaac agatgtttct tcgcgcatat
    44581 gtgacagccg atcagttgac aaaaataaaa attggtcaga aggtaaaagt tctggcagaa
    44641 tcgggcgaag atgacagccg cgagtatgaa ggaatggtta cgtggatttc ggataaatcg
    44701 gagtttacac ctaaaactat tcaaactcag gatgagcgcg ccaatctggt ttatgccatc
    44761 aaagtggctg taaaaaacga tgggtttctt aagattggaa tgtatggtgg aattgttttg
    44821 ggagattaat acatgtcagt tgttgttcaa aatatcagta aaaagtttgg ccttgttcag
    44881 gcattgaacg atgtcagttt ttctgcgtct aagggcgaaa tttttggcat aatcggaccc
    44941 gatggagcag gtaagaccac gcttttcaga attctcacca cgcttattct tgctgatggc
    45001 ggaaaggcct ttattgatgg atttgatgtt gtgaaggatt ataaaaagat tcggcatctg
    45061 gctggttata tgcctgggcg tttttctttg tatcaggatc tgaccgttga agaaaatttg
    45121 aatgtttttg ccagtgtttt taaaacgacg attgaagaaa actatcatct gatccgcgac
    45181 atttatcagc agattgaacc atttaaaaag cgtcgtgccg gagctctttc gggaggtatg
    45241 aagcaaaaac ttgctttaag ttgtgcattg attcataagc catcggtttt gtttctcgat
    45301 gagccgacga ccggtgttga tccggtttca cggaaggagt tctgggagat gctgatcaaa
    45361 ctgaaacagg aaggaattac aattctggtt tcgacgccct atatgaacga ggcggctttg
    45421 tgcgatcgca tagctttgat tcagtcaggt aagattttca aagccgatac accacaaaat
    45481 attgtcagga gttttggaaa aacattatgg gcagccagga gcagcaatat gtcaaagttg
    45541 cttaatgatt tgaaaaatca tccggaaata gaaacctgtt atgcttttgg tgactcaaat
    45601 catttgataa tgaaaaatga atcgacctca ggggagttgt tgaagcagca acttttggca
    45661 aaggggcatg aaaaggtcga tgtttttccg atcgaagccg ggatagaaga ttgtttcatt
    45721 tatttaactt caatcaaacg atgacagatg taattgttgc agatcagctc acaaaaaaat
    45781 tcggatcgtt tactgcagtt gatcatatat catttcatgt tcaagagggt gaaatttttg
    45841 gttttctcgg agccaatggt gcagggaaaa ccacggcaat gcgcatgttg tgcggactca
    45901 gcaaaccttc ttcgggaaca ggactggtag ccggtttcga tattgctcac caaagtgaac
    45961 aagtaaagaa aagtattggt tacatgagtc agaaatttgc tttgtacgaa gatttgaaag
    46021 tttgggaaaa cattcgttta tatgccggca tttacggagt tccggaaaaa gaaatagcac
    46081 cccgaaccga tgaaatgtta tcgtcgattg attttttgtc tgaaaaaaat acattggtca
    46141 aatcgttgcc tttgggctgg aaacaaaaat tggctttttc gatttctatt tttcaccggc
    46201 cgcaaattgt ttttctcgac gaacctacca gtggtgttga tcctgttgtg cgcagacaat
    46261 tctgggaatt gatttataaa tcggccgacc agggtattac ggtatttgtt accacccatt
    46321 atctcgatga ggcagaatat tgtaaccgcg tttcaattat ggtggatgga aaaattgcag
    46381 cgcttgatac acctgcagga ttgaaagaaa aatccggcgc cggcactata gaagatgcat
    46441 ttcatcagtt aacacgaggc gcaacacgca aagcagatta atatgagaca gtttttaata
    46501 tttacacaaa aagaattcta tcatattttc cgtgaccggt ggacaatgat tattttgatt
    46561 attcttccgg ttgtgatgct cattttattc ggatatgcca tcaccactga aatacgcaat
    46621 tcgcattttg ttgttttcga cccttcgcgt gatgcagcta ctcagggcat tgtcaataaa
    46681 cttgaaacca gtgagtattt tgtttttgat ggttatgtac atacaccggc cgaagctgaa
    46741 gaaattttta tgcggggcga aataggaatg tttatcattt tcagcgaaaa tttctataac
    46801 aatatgctgc atacaggcaa tgcgcaaata agaattgtta cagatggatc tgacccgaat
    46861 acagcccata cacttacttt gtatgcacaa aatctcatgg cttcgtatca gcaggatatt
    46921 tttaaatcga tggaaatccc atatcagatt gaacctcaaa tccggctctt gtataatcct
    46981 actatgaaag gtttgtacaa cacggtgccc ggggtcatgg gaatgcttat catgctggtg
    47041 tgtgccatga tgacatcagt ttcaattgtg cgtgaaaaag aaaaaggcaa tatggaaatt
    47101 ctgcttgttt ctcctatgaa acctatcagt atcattcttg cgaaagtagt tcctttcttc
    47161 atgatttcgc tgatgaattt tacaaccatt attgttattg caacaactct tatgggtgta
    47221 ccggtcaatg gtagctggtt tttgctgatt gccgtttcat tgcttttcat ttttgtttca
    47281 ttggccattg gtattttgat ttcaacgctt gtgagtaaac aaatgattgc attgcttgct
    47341 tcggctatgg gtttgatgtt gccggccgtt ttattgtcgg ggttggtgtt tcctgtttca
    47401 agtatgccaa tagcattgca atgggttgct caggtgttgc ccgtgaaatg gtatatcgaa
    47461 gcaatgcgat ctattatgat tatgggcgtc ggtttttcat cagtctctct gcagtttttt
    47521 atattgtcgg gaatggctgt attgttactt gttttaagtt tgcggaattt taaaatcaga
    47581 cttgattagc tatgttgaag tatcttcttg aaaaggaatt caaacagttt ttccgcaaca
    47641 aagcgttgcc gaaaatgatg gttattatgc ctcttatggc tgtacttatt tttcctttgg
    47701 cagccgattt tgaaattaaa aacattaatt tgtctgtagt cgatcacgac aaatctgcgt
    47761 attccagttt gctggttcag catgtgcagg cctcaggtta tttcaggttg accgatgtat
    47821 cagagtccta tggcgaatca ctgaagtcgg ttgaaaaagg aaagtcggat atgatacttg
    47881 aaataccttt gggttttgaa aaaaatctgg tgaaagaaca tcaatcagat attcttatcg
    47941 cagcaaatac tgtaaatggc atgaaaggtg gaatcggcag ttcgtatctg gcaaatatcg
    48001 tccgcaattt cagttccgat attcgtttgg aatgggttca gggcaacagc cgattaaatg
    48061 ccccgcaatt tgaaatcgtt ccagactatc gtttcaatcc ccaattgctt tatcgtgtta
    48121 cgatgatacc agccattatg attatgattc ttgccatggt ttcaggattt ttacccgcac
    48181 tcaatattgt tggtgaaaaa gaagatggca gcatagaaca gatgaatgtc actcctgtaa
    48241 aaaaagtgta ttttatttta tcgaaactta tcccatactg ggttcttgga tttgttgtta
    48301 tcagcatttc atttcttgtt gcctatattg catatggttt aattcctgtc gggaatctgc
    48361 ttgtaattta tttgtacgcg tcgttgtttg tacttgcttt ttccgggttt ggacttgtca
    48421 tttccaatta tgcccagacg gttcaacagg cagtttttat gatgttcttt tttgtcatca
    48481 catttatttt tctgagcgga ttatacacgc cggttgaaaa tatgccggag tgggctcaag
    48541 ccatcagcac tttcagtcca ttgaaatata tgattcaggt attgcgtctg gtttatttga
    48601 aggggagcgg gtttgcagag ttgacaaccc agttgatagc actgatttgc tttgctgttt
    48661 ttttcaacgg atgggcggtt ttgagttatc ggaaaagtca ttgacgattg attataaact
    48721 gacgttttgg ttacgttgcc ttttttatat ttttaatttc gttgtttgat gggttagccg
    48781 gatacgtttt ttgaacgacg tatccggttt tttttaaatc atttgaaact cgcactgaat
    48841 gcgcttaaac agaaattgaa ttttcagttt tatgttattt gtttttttcg accaaagggc
    48901 ataaaaattt ttttcgtgtt tttattgttt ttttttgcga caaagagaaa aatggtcatt
    48961 aaaagatgat gatttaaggt tgtattttgg aaacacatgt atttatgcgg gtttcagaat
    49021 gtatgttttt cgggtttttg taattttttt tttgaaaaag atgtattttt tttccgaatc
    49081 aaatactttt caaacagaga ttgtgaataa ctacaagcgt tgttttttgg aaaacgtgtg
    49141 tataagccaa ttttcgcatg tttgatattt tttcgttttg ttgaaaaaaa gtgattgaca
    49201 aaaaacaaaa aatattgtat cgaaaaaaaa atgattattt ttgctgcgaa tataaaaagt
    49261 tttgaaacag aattttatgg catcgaagaa acgcgtttac atttttggaa acaaaaaagc
    49321 agaaggaaaa gcagatatga aaaatctgct tggcggcaaa ggagcaaatc ttgccgaaat
    49381 gaacctcatc ggacttccgg tacctgccgg cttcacaata accactgaag tgtgtaatga
    49441 atattatgcg attggtgaaa agaaaatgaa ggatgtcctc gggaaagaaa ttaaagacgg
    49501 attaaatttc attgcgaaag taatgaacgc cggattcggt gacaaaaaaa atcccttgtt
    49561 gttatctgtt cgttcaggtg ctcgtgcttc gatgcccgga atgatggaaa ccattttaaa
    49621 tgtcggatta aataatgaga cacgtgatgc gttgattgca aaaacgaaca accctcgttt
    49681 tgtgtacgac agccagcgtc gtcttattca aatgtattct gatgttgtaa tggaaaaagc
    49741 tgccggcatt gaaccagccg aaggtaaagg cattcgtcag caattggaaa aagaacttga
    49801 aaaaataaaa gaacaacgtc atgtaaaatc agatactgat cttactgccg atgatcttaa
    49861 aaatctcatt gaagtatata aatataaggt aaaggaagtt ttaggtaagc ctttcccgga
    49921 agatcccatg gaacaacttt ggggtgctat ctttgctgta ttccgcagtt ggaacggaaa
    49981 acgagctatc agttaccgtg ctatcgaagg cattcccgaa gattggggaa ctgccgtgaa
    50041 tgttcaggcg atggtattcg gaaacatggg cgatacttca gcaaccggag ttgcatttac
    50101 ccgcaaccct gccaccggag ataattattt ctatggtgaa tggttagcca atgctcaggg
    50161 cgaagatgtc gttgccggaa tccgcacacc aaatcccatc aatgaagttg gtaaaaatgc
    50221 gcacaacgaa catcaggtga gtcttgaaaa aggaatgcct gttgtttaca aagaactcga
    50281 cactattcag aaaaaactcg aaaagcatta taaagatatg cttgatattg agtttaccat
    50341 tcaggatggt cgtttgtgga tgttgcagtg tcgcgttggg aaacgtaatg gtactgcggc
    50401 tgtgcgcatg gctttggata tggccgacag caaaatcatc agtgaaaaag acgccgtact
    50461 tcgtgtacag ccatcacaac ttgatgaact gcttcacccg attctcgatg ccaatgcaga
    50521 aaaaaaagcg aaggtgatta ccaaaggttt gcctgctggt cctggtggtg ctgtcggaaa
    50581 gattgtcttc acatcgaaag atgccattga ttatgcaaag aaaaatgtca agtgcattct
    50641 ggtacgtgaa gaaaccaatc ccgaagacat tgacgggatg cgcgctgcag ttgctattct
    50701 cactgcccgc ggcggtatga ccagtcacgc tgctcttgtt gcacgtggtt ggggaaaatg
    50761 ctgcatcgtt ggtgcgggtg aagtgaagat tgatgtacac aaaaaagtga tgattgctga
    50821 tggaaaagaa tatcacgaag gtgatgtcat ttcactcaac ggcagcaaag gtcatgtgta
    50881 tgaaggtgcc atcggaatgg taagtgctgc agagaatgaa tattttgttc gtttcatgaa
    50941 gctggttgac aagtaccgca tcatgaaagt acgtaccaat gctgatacac ctgatgatgc
    51001 taaaaaagcc ctcgaatttg gtgccgaagg tatcggattg ttccgcactg aacatatgtt
    51061 ctacggacgt ggaagtgaaa atgctttgtt ctcgcttcgc aaaatgatta tttcgaatac
    51121 aaaagaagaa cgtcagaaag ctattgacga attgtattca tttgtaaaga aagatatcaa
    51181 gggaacactc gaagctatgg atggatttcc ggtaacaatc cgtactctcg atccgccgct
    51241 tcacgaattt gttcctaata aaaaggaagc ccgcgaagaa cttgcaaaaa gtctgaacat
    51301 ttcaatggat gaattctgca aaagagccga tttgttgcac gaaaacaatc caatgatggg
    51361 acatcgtgga gtgcgcttag gcattaccta tcctgaagtt accgaaatgc aggtaagagc
    51421 tgtttttgat gcagccgcag aacttcttaa agccggtaaa aaacctttcc cggaaattat
    51481 gattcctgtg acttgtgttg aaacggagct tgctcatcaa tatgcgatag ttaataaggt
    51541 atattccgaa gctcttaaaa aacataatct gaaagcaatt cctcatctga ctggaacaat
    51601 gattgagatt ccgcgtgctg cactgaatgc cgacaagatg gcacaatatg cgcagttctt
    51661 cagtttcgga accaacgatc ttactcagat gaccttcggt ttttcgcgcg atgatatcgg
    51721 tggctttttg cccgattatc tgacgcagaa aatattgccg gtagatcctt tccaggttat
    51781 tgaccgcaac agtgtgggtg aattgatgaa aattgcgtgc gagcgtggtc gtcaaaccag
    51841 aaaagatatt aaacttggta tatgtggtga acatggtggt gagcccaatt ctgtggaatt
    51901 ctgtcacagt ctgggcatgg cctatgtaag ctgcagcccg ttccgtgtac ccatcgcccg
    51961 cctggctgct gcgcaggctg ccataaaaac tgaaaaagca gcaaaagaga aaaaaggaaa
    52021 cccaattaaa aacgcaaata aaatggaaaa agcaaccccg aaaaaaaagg ttgtagctaa
    52081 aaaagctgcc cctaaaaaag caactgctaa aaaagcaact gctaaaaaag caactgctgt
    52141 aaaagcaaaa gctactacca aaaaagctac tgctaaaaaa gttgtaaaaa aagttgtagc
    52201 taaaaaagcc gctgctccaa aaaaagctgc tgctaaaaaa gtagtagcta aaaaagtcgt
    52261 agctaaaaaa gctgctgctc caaaaaaagc tgctgctaag aaagttgtga aaaaagctgc
    52321 tccaaaaaaa gctgctgcaa agaaagttgt agctaagaaa gttgtgaaaa aagctgctcc
    52381 taaaaaagcc gctgcaaaaa aagttgtagc taagaaagtt gtgaaaaaag ctgctccaaa
    52441 aaaagctgct gcaa
//
LOCUS       DAOPKE010000011        48164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_767_length_48164_cov_13.436592, whole genome shotgun sequence.
ACCESSION   DAOPKE010000011 DAOPKE010000000
VERSION     DAOPKE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_48164_cov_13.436592"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(658..1959)
                     /locus_tag="PKY63_02790"
     CDS             complement(658..1959)
                     /locus_tag="PKY63_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99571.1"
                     /translation="MNKFFLLSFVFLSSVCFAQVIHPPIGTISNPDGAEVELTLISRI
                     HSHTPKVMDNMERYEKDIISPKSAVFLEEKNKFYINSLEGFMTVVFDLKTFTKIKSIH
                     HDFAKKDAKLFKETTVLGYNFLTPREEPNIFKGKPVECCLSNNRKYLWVTYYRRSYDP
                     NAMEPSAVAVIDTDKDEIVRVMPTGPLPKMIAASPDGKYVAITHWGDNTVAIIDVSSG
                     DAEKFKYVKHLVVDYQQKFSASQNQGGIDRDKHCGLCLRGTAFTSDSKYLLVGRMGGG
                     GISVFDMSDFSFKSTVFGMRTNVRHLVVTKNHLYLSSNRSGYVQKCSIADFIAFAINP
                     DNKGENFTAWQECFAGAGARTIEVSDDEKYIFAACNNASAVVIIRAKDMVKVGEIKAD
                     SFPVGLALRKNNYLLISTSQAHPGVGGGNCVDIYYVRYNKK"
     gene            2347..2928
                     /locus_tag="PKY63_02795"
     CDS             2347..2928
                     /locus_tag="PKY63_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPE99572.1"
                     /translation="MKLFFGFLVSLTLIFGSSVYAQTDLAMLSHPDSAITEIKPDSAN
                     ISRPADSLALRSYIRDSLVNELITYSKTFLGVPYRYGGKSRAGFDCSGYVSTMFSHLG
                     ITLSPSSVGMAGSGRIVADSTIRPGDILFFINTQRRRSGIGHVGLVVEVKDGKIVFLH
                     SACNGGVRYDYLSSPYYKSHYYRAERIPLFDVE"
     gene            2943..3557
                     /gene="rsmG"
                     /locus_tag="PKY63_02800"
     CDS             2943..3557
                     /gene="rsmG"
                     /locus_tag="PKY63_02800"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPE99573.1"
                     /translation="MELIKKYFSGLTETQLSQFAQLEPLYNDWNSKINVISRKDMEHF
                     YLHHVLHSLAIAKVFPFTPGMRILDVGTGGGFPGIPLAIFFPEVQFTLVDSIGKKIKV
                     VKEVASALELKNVEATQARAEEMKGQWDIVVSRAVTDLPKFMELVRNRITRKPKGNQP
                     KIIYLKGGDIDAEMSGSDYPYSVAPISGFFSEDFFSTKLVVKIF"
     gene            3722..5383
                     /locus_tag="PKY63_02805"
     CDS             3722..5383
                     /locus_tag="PKY63_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE99574.1"
                     /translation="MVENAQKTLRDNPAIRWTALVLLAFAMFCSYIFMDILSPIKDLM
                     QSTRGWDSTAFGTMQGSETFLNVFIFFLIFAGIILDKMGVRFTAILSGLVMIIGAAIN
                     WYAVTENFMGSSLDVWFTNNLNYIPGFNELGIAPFYSGMPASAKLSAVGFMIFGCGVE
                     MAGITVSRGIVKWFKGRELALAMGSEMALARLGVATVMIFSPLFANLGGNVDVSRSVA
                     FGVILLMIALIMFIVYFFLDKKLDAQTQESEEKDEPFKIKDIGKIFSSMGFWLVALLC
                     VLYYSAIFPFQKYAVNMLQCNLTFTEVEKGTFWASNNVTMLQYLIMLIVAGTAFISNF
                     SKKTMHYSMLTISVIGLIVFCYMGYMRQSAETIFAVFPLLAVGITPILGKYVDNKGKA
                     ASMLMIGSLLLIACHLTFAFILPMFQGNAIGGVMIAYLTILILGASFSLVPASLWPSV
                     PKLVDQKVIGSAYALIFWIQNIGLWLFPMLIGKILDNTNPQVVADLEAKIITPEQAAI
                     SYDYTAPLIMLAGLGVAALILGIILKRVDRIKGLGLELPNIVKKD"
     gene            5529..6536
                     /locus_tag="PKY63_02810"
     CDS             5529..6536
                     /locus_tag="PKY63_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99575.1"
                     /translation="MKTKNLLLITVVFAAFLVSCVPARQLQDEKTRREKAESELAAMR
                     ADFDKMSATNTELNENMTTMTKQIAALERDTSITGISLRKMTNQYDKVNSLYEELNRK
                     YEELLKINASETQKISTELQLTQEQLAIKEAELKKLENELNIRKQNLDQMQADLLLKE
                     KRIMELESIINKKDSIMKELRNKLTVALKGFENNGLTIVEKDGKIYVSLDESLLFASG
                     SWTVNAKGKDALMKLSNVLADNPDIAIMVEGHTDNLAYKGSGQVKDNWDLSVMRATSV
                     TKIILNNKSVDPKRIISAGRGEYFPIDGANNSEARQKNRRTEIILTPNMDEFMKILQN
                     N"
     gene            6787..10068
                     /locus_tag="PKY63_02815"
     CDS             6787..10068
                     /locus_tag="PKY63_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99576.1"
                     /translation="MKHFCIISIVCFLMNAGLQAQEEYIQPRKHLLDGIEQHDKGEFS
                     KALKAYNKINCNDSLYPLALYEKSLSYNALEEYDKAIEVLNELLSKSDSTSQILISLA
                     SAYDDKKNHAEAQAIYKKVIDRQPYNQNAWYNLGLSYYNDKQYLKAEECFIQSIKLKP
                     WHFRSNLILGLTNAKMGRPVHALACINLAALMNAGDNFGHSALQEYEKFVHGDYEADI
                     EYDKYVTPDSITTDKIRALEPIIKSQVLQSKKLKMPSKNPMTITKYNYVIFSELPQTK
                     EKNNIYTAYYFPFLRSILDNKYFDVYSYFITQSVNNKNIIKYVLTKKSKLNAFSGWMI
                     KELQKYFASPFFRPSNVDYAYAEGNLIRITTYSDSTKTVKDGKYIELDENGDLEYTGT
                     FKNDKHEGTFNSYSVDGKVRVFVKYKNDDEYLLVSNPEVIKGNQFEVEMKDGKKNGWY
                     KTFFPNGAVKSIELYKDDEFVGDGFELYIDGDTMTTYKPDADGLHYTTTNYYRNGKIS
                     TCYKRLIDSYLYDGEYISNFYNGKLARKITLKDDLFIGKTFTLFLNGDTAAIQKYDDK
                     GNATGIHSSFGTSGKLISSIEYQKGKSNYIFTYYHRNGKPQSQLKVSNNKFNTLVYYD
                     STGKEIVHHDNLNRNLNEYTNVNVFTKKIIDHFVLKDGLRDGPAKHYTHSGTLESECK
                     YSKDEIDGVNVTYYPDGTKRSEYTYEEGVLKGKAISYYSDGKIESEGFYDDDNKVGEW
                     RYFHADGKLSNTEYYNTSGELNGEQIEYCGDSIPVSVFTYREGNIIDLVFYGPDMDTL
                     EHYSLPNGFGKNTIYYKNGKLKTTRNYEHHVLHDSVVYYYPNGQLYYKINYVNGFRHG
                     RYCEFNPDGSKKRELDFEYGQRCNENNFYLENGFPDASLICKNNEENGPATYYHYNGK
                     PTAIINFSNDEKNGFADYYEPGGQLMYRVWYESNEAVSVTWLGKDKKFLEPVLIDKDT
                     INITTYYASGSKSAELSFVGGMRHGEFRGYFPNGKLLEIRPFKNNYLDGKEICYRDNG
                     ILFAETEYLIGDKHGVCTVYHPNGKPAVRTEYYFGERKGTQTYYNDKGQVKNEYQFYG
                     DRMLE"
     gene            10046..11986
                     /locus_tag="PKY63_02820"
     CDS             10046..11986
                     /locus_tag="PKY63_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPE99577.1"
                     /translation="MATECLNRTILTWLCLIALSLGDSFGQTEYDYEYLKEKYSSENT
                     VVLTSRKEISVIRKKKKIVIVEKVFLEKLMTGKNKDEAQLDIIPFSTFASISSIEAYT
                     LVPDKNGWKKHMAWNFRDMLHVGSYLYNDNYLKIIKFPAVEQGCRTIISYTKTYTEPY
                     LWGGHFLSNGETVLNGEITLKAPKSITFNIKTHNFQQDSLLFELESKGSWNMFNWQYK
                     TEKTKKPDDQPLFFSGPMLLMNIARDTDKKHGQYYLESNKYLANWYFHNFLKAKNQRD
                     TVMEEIADSITNGLNYTGEIAMTMTNWVSKNINYIAIEDGYNGIIPRSSNYVLKQRYG
                     DCKDKSNLLIEMLKHKNIRAYPVFVGTTSLPFSHDSIVSPIVDNHVIVAIDTGKNNIL
                     FVDPTQEYQIEGLPPFLVQGKTALVLTDSMDYFIEHFPTPDPELSKLTMTNEIFIEGP
                     TLFQQTDLTANGYFMVFINPMLTDRPEDKIKGDCEFISFPESKTQIPDSLSDLELNPD
                     SAFVSFTIFSHQANPLQKTNSGIFINPYPNGFFTLKSFQELEEKKQSVFHFPATWESI
                     TTLHLENDYHVKYLPQNILIDSLHYSFSLDHSKEESDVVIHCILRINKLYFTDSELDG
                     LASFFADIRRAFNQNIIITPKN"
     gene            11990..13954
                     /locus_tag="PKY63_02825"
     CDS             11990..13954
                     /locus_tag="PKY63_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPE99578.1"
                     /translation="MKLLIFFIAVAGVLIASAQRPENKSYSWEATPQFVQSDSVEAKH
                     DAVYLKTKIIYDFYFEKESGDLAELFVFHRAYKICTQNITEDLNTIKVSTSDFNNMLD
                     LKVRVIAPDGKITDVDTSEFLTLNDEVNNGDYKVLALPNVSRGSIVEFYYVLESSPTY
                     FISRSFQYGIPSFDVSFKVYSPINLGFISKSYNGFPELEEKTDSTEEYRVMSAHTDYI
                     PAFQKQGYSYSSPHEQRVLMSIGYNFARSKARLFTHAEFCGNLYERLFVLEKKEQKAL
                     SKIVKKAKSKDPDKLIAAREIENYLKATYFNELNIGTVTETLDFIESTRFGSDIGIVK
                     LYMNIYKTLGYKCNLLLTCTRDYMYFDKSFNTYNQLNDYVLYFPEFDLYMSPLHFTSR
                     LGFPDNLVYEQDALFIKETTILGETSFVPDYTTIKKPDYKSSADSVYQILTINPDCKT
                     ASLKCHRSLTGHKASYIQGIYSYMDDEEREELLKEYFDYGHVYFNPDSAIIADTSKKV
                     AGIKPFTIDANLNGSNLLSQADDHLIVLAGELIGPQAELYDSVQRTLPIDHGYGCQYI
                     RTLEIVIPEGYTVENAADFDINLKLNSKTGEQTAWFISKQRLEGNTFIIDIDESYTEI
                     KYDLADYEQFRNIVNAAANFNKIKLVLKKS"
     gene            14153..15415
                     /locus_tag="PKY63_02830"
     CDS             14153..15415
                     /locus_tag="PKY63_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPE99579.1"
                     /translation="MDSRPLAEILRPLTTDDYVGQEKLMGANGIIRRMLDNKAISSMI
                     LWGPPGVGKTTLALLIANVLQRKYHQLSAISSGVKDVREVISAATSEQSPILFIDEIH
                     RFSKSQQDSLLAAVEKGTITLIGATTENPSFEVISPLLSRCQVYVLESLSSDHLNILL
                     EKALQFYKKEGYTISVKETDMLFRFSGGDARKLYNALQLVVENSLKNNSAEITNDKVA
                     RVIQNNLARFDKTGEMHYDIISAFIKSMRGSDPNAALYWMARLIEGGEDPKFIARRML
                     ILASEDIGLANPNAILMANACFDAVHKIGWPESRIILAECAVYLASSAKSNATYLGID
                     AALEHVRKTGDLPVPLHLRNAPTKLMKELNYGKEYQYAHDFENSFVNMEFLPEKIKGT
                     KFYEPGKTAREEDLRTFLKIRWKDKYGY"
     gene            15517..16248
                     /locus_tag="PKY63_02835"
     CDS             15517..16248
                     /locus_tag="PKY63_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPE99580.1"
                     /translation="MILSVIIPAYNEEKYIAETLSEVYGLSFPAIIEKVEVIVVDDCS
                     KDGTTNILNAQKTKHPALIVHRNEKNMGKGASVKKGFEISRGDLIFIQDADSELSPGD
                     IPRMIDAMLELKIEFVNGSRYLPGIVRPVISYKRYFANRFFTFLVSFLSNAKLTDIAC
                     GHKLIHRKLLEKIPIRSKRFGFEAEIMIKALKIKKNNIAEVPVMYRPRSVREGKKLKS
                     SDGIKILFTILKYEFFWCPAAKNKS"
     gene            16208..18019
                     /locus_tag="PKY63_02840"
     CDS             16208..18019
                     /locus_tag="PKY63_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99581.1"
                     /translation="MNFSGVLQQKTSHSKIPGLIIIALVLFSAVFAHVEKRAHKSVIS
                     DDVISYYAYLPALFIHHDLTLEFANEDPAYYFKYYWPGKTSNGKYVIKTTMGVAFLYA
                     PFFGTAHLYTLATNGTADGFSRHYQTAINIAALFFLFIGLFFTNKILLRFVSAKVSAV
                     VLVLITLGTNLLYYATMEPGMTHVFNFGLIAMFIHYSDKWLLKPDFRNTLLCGLLFGL
                     ITLIRPINALVILFPFLWNVSSLKELQNRLLFFIKQWQLILLIAGCVLLVWMPQLIYW
                     KIQTGQVFYNSYNEHFYFHNFHIHKALFGFRKGWFVYTPIMFIASLGILFLRRQFKPV
                     QLSVAVFFMVFVYITFSWWCWWYGGGFSARPMTDIYSIMAIPLSVFVAKGLRAKKSIA
                     IITFLFTTLFAAHGVYQTLQYVRGSIHYDSNTWHSYKNSFGRIKPGPNYYYLLSPPDY
                     INAIEGKPEETAEENTDLIAGPNDYYIEMNDIRTSNNTEYFNIVNINLDTSFITTATI
                     VIPAFDSNDFCDDLTLVTAIYTPSGEAAYFSSVAVAATTRYGDKQLVENVELNIPLYV
                     TEKPNIKVFFRSIEKDDFSCKKVKVLIRGIRLHYTDV"
     gene            complement(18033..19169)
                     /locus_tag="PKY63_02845"
     CDS             complement(18033..19169)
                     /locus_tag="PKY63_02845"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99582.1"
                     /translation="MKKRRDEIVKYLLVAGVFLLMTGPFINNKLKVIDATPLRGAVNE
                     PVGPEFSTHGWFSGEYQVQKETYLNEAFGLRSVFVRLNNQIAFSFFSKAKANGVIIGK
                     DNYLFEENYIKAYNGTDFIGVDSTVRRMKRIKFVQNALADRGKTLLIVFAAGKGSFYP
                     EYFPDDLKQRKGISNYQLHSHLAEYLNLNYIDFNSYFLENKHSSQYPLYPKCGIHWSY
                     YGSCLAADSMISFIERKRNIDMPDLRWDSIEVCQPKFEDYDIGDGMNMLFRIGSFDMA
                     YPKLYTEDTTGKSRPSLIVVSDSYYWEMFNYGFPRSFGNNQFWYYNKGVYPEYYTNQL
                     EASQLDLAQSVADHDVFIIMATEATLPDFGWGFIERLYVFFGGK"
     gene            complement(19171..20595)
                     /locus_tag="PKY63_02850"
     CDS             complement(19171..20595)
                     /locus_tag="PKY63_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPE99583.1"
                     /translation="MVFSSIVFLLYFLPAFLLTYYFADKKYKNFVILAFSIFFYSWGA
                     PKFIFVILGTTFLDFHLVKWMSQTKNDLHRKLMLTLSVSVNLGLLFYFKYSNFFIENV
                     NAALSVFGGGEIQWTKLILPIGISFYTFETITYVVDVYRRVHAPLKNFWDYQLYIILF
                     PKLIAGPIIRYHDLADQIGDRSEYETKDNVLTGFYRFAIGLAKKVLIANHMGQQADMI
                     FAMDYSGLDTATAWIGILAYTFQIYFDFSGYSDMAIGIAKMIGFRFPENFNNPYVSRS
                     ITEFWRRWHISLGAWMRNYLYIPLGGNRVKTQRRLYFNLWFVFLASGLWHGASWSFVI
                     WGAYHGLFLVLERGFLLKVYERIGKLPATLLTFFLVVIGWVFFRIENVSEAFLFLNRM
                     FALDFTTLPVLDVEFVVFLFLAVFFAFFTFFKVGEQIQNAIYFNTYSVKKHLSVAAAV
                     FVLFILCISSITASGFNPFIYFRF"
     gene            20757..22316
                     /locus_tag="PKY63_02855"
     CDS             20757..22316
                     /locus_tag="PKY63_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99584.1"
                     /translation="MKSVRHIIILAFMAGLLASCAKDPSWDVDVRTPLFNTSLGVNDL
                     IADSMLIENPDTSLTFVMDFDLFNITADTFVNMPDSLFYAHYVMPVGLTVPPGTLVLQ
                     KTESRYYNLGGALLTQMRIKSGTLNIRAYNYVGDAAYIEYSIDNSMLNGAPIVASGLI
                     PSFPATGEYLETQLDVSGMYLDLTQSYMHCNSLKSTMKVFANPNATTDIVVNFNDSIN
                     ILVEFKNIVIDYSRGYFGQHEINSISAESFPFIEDLGIDYLDMENIAMTLTMENHLGA
                     DATFTINELAGHGITDIALNSEWIGKAINLTRATENPVNSGNVTPSVFEMNFDASNVE
                     AFFENLPDSISYDFSGTVNPLGNISTGNDFVYYGQGLRGKIHAEIPMNIAVQGLTLSD
                     TIDYELNKSNNFITQSELFLDISNGFPLTASFKIFLTDEADNVIDSIIPDNNIPSGTV
                     NAEGKVTAPSLTQMKITLNENQTTHLYACKKAIVVAKLNTGGISGQKVKFYPWYHLNV
                     LATGLFNIFIE"
     gene            22313..23512
                     /locus_tag="PKY63_02860"
     CDS             22313..23512
                     /locus_tag="PKY63_02860"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPE99585.1"
                     /translation="MKSTFVFFLILMTYFLAAQKNTSPEIRIESWNALESKTLSSDFF
                     SSFYFGEYIDSSSKWNEIEHLKEKNHIGFFSNHRIEVMLPSEKKHSLYFSGSHQSLLG
                     AGFSKDLFQLVFTGNNSLIGQHVITNPATMNQYSFSTLSAGIAWQINDDMKIHAAAGP
                     VVLYSFSRLDFSESDFFTSLSADSLSLNLDGHYMRAGGNTFIKGSGFGAELGIEGTNN
                     NFEWRVTLSNIGQIWMNKRTIDSQHDTLLFFTGVEISDLSNFSAVIDEELDNFEESFS
                     LKGDTANTSISLPMLITGECRAEFGKLRTDLNILYYNLPGFIPFVQIRPSWPLTEGIR
                     ISLPIKYGGYGSFNAGPGLEFKIFEKLSAQLDFPAALSVLNINRNLSYVVVGRITYKI
                     TEHASLL"
     gene            23496..24926
                     /locus_tag="PKY63_02865"
     CDS             23496..24926
                     /locus_tag="PKY63_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99586.1"
                     /translation="MRVFYSILLFFAVALGFAQDAFIRTYGLDGSFNEGKGIAAFSDS
                     TYIMLGNRQTFGGQSAAWLFRVDSNGTILWEKYFDSYVISMAENLSKHDDTSVIITGT
                     VLAGNDYSMLVARVGLSGSVIWEKIIGTQSWDQALCSASDADGNIYLSGYGQAIDTMD
                     QDILLYKLDGNTGDSLFSKRLDAGYNDKGVYLDTMSGNNLLLISHSYKPFSDSVMSRV
                     WKMNVDFDTLWTWMPCDSAEFEINCIFEDTFHRIVFSGKMLPDTGQVFRFWYGFLNDS
                     GIFGWQLIHPPYFLKNIRRGILDSNNVSYYTGGFFPAYFGFGNSDIGFWRDSLGWGFS
                     HNYGALQDEEGMDLDFAADGGMVFIGTTKNYGEGVNNIFFVKVGPDYLYNDSDYAHYT
                     PVSPSENMPAAIYPNPSSGTFCITLPNIQECKIDIYNLQGVLIKTDRYNSGETYTLKG
                     FENGVYLIKITSDHQSYNFRLLLQND"
     gene            24928..26091
                     /locus_tag="PKY63_02870"
     CDS             24928..26091
                     /locus_tag="PKY63_02870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPE99587.1"
                     /translation="MNMKQKKILVFAAPVFLVALAVILINPFGQQSAVFGKISPVAES
                     DSVTFWVTGDAMMHMPQYKAAYNENTGLCEFDSCYTYLRPILQNADVRLINFETTLAG
                     EPYSGYPKFSAPVEYADALRRAGFNFFVFANNHSCDRGAKGIEGNIDYADSLKLWHTG
                     IFRNPTERLARFPYMLEVKGWKLAILNYTYGTNGIPEPEPYVVNRIDSAQILSDIKKA
                     KSQSADAILVSIHWGEEYQHKQNANQKALANFLLESGVDVIIGSHPHVVQPMELVTPI
                     GSMKERLVIYSLGNFISNQKDPLTDAGLLVGFTLKRTPYGKPEVCNVKFIPLYRMKLE
                     GKKPGYYMMPGLMTEKNIDALIPGDENKEDFRSVMEDTRRKVNTDIRIREVEK"
     gene            complement(26524..28143)
                     /locus_tag="PKY63_02875"
     CDS             complement(26524..28143)
                     /locus_tag="PKY63_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPE99588.1"
                     /translation="MKAFRILMLLIAVFIINHAGAQTITTKPQVYAVTVYMLGAELHC
                     SAEAVLPKGKCTVLLPGLSPQLNAGSIRFSVENSDATILSVSSRINYLNGQGDNKKIR
                     LLKDSVELINDRLTRIAMSRETFVKEKDLLFKNQSIGGTENGVKVEEIQRSADFFRLR
                     SNQINDELFVLAKNESKYRETLARLMAELDELNATYNPPSSEIELSVLSQREEKIKFD
                     FSYMVSGCGWAPKYDIRADGVDKPIQMVYRANLFNNSGVDWNDVKIKLSTAEPGKSAT
                     KPILDKWALNYGYDVQDASESNVMNYDQRMTIEATKVEGAAIAGYETIRVDELSQEFE
                     IAVPYTILSDSKAYTVDVNTFDLPAVFEYSAIPKMDRDAFLVARITDWNKLNLVSGHA
                     SVFYAGTFVGQSYINTSTVADTMSISLGRDKRIIIKRAQKMEDSKSQVIGSSIKETFM
                     YEIIVRNNKECAVNITLQDQVPVSQNSDITVDVINISGGTLDPLSGIITWKMTLQPSE
                     TKLLILNYSIKYPKNQRVDKKRSRAISCPSF"
     gene            28219..29085
                     /locus_tag="PKY63_02880"
     CDS             28219..29085
                     /locus_tag="PKY63_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99589.1"
                     /translation="MKPIIPLLFAILPLIATAQNLSVSPFFTSSKGLLSTTWHSTNDH
                     TFVTIDTSITKYSGESTNISLGAGNRYGVNIEKKYRDLISFGLSLSYFSSKDVPLLCT
                     SKAEYDPYSAYNLSFTTTDIYKNKSFDLGIYSNFIIYNKSTAPYVKTGLIFSFSKYTL
                     DREVYIFNNIPGYYPTERYNYNYKISPSFHYGFTGAAGIETGRNKTISLYAEIYSVFM
                     NVSPTKQICTAKTWNSEDQFETMTTSDKETVFVESYSEDDNKNEDEPTKNLKYNQSFC
                     SIGLQAGVKIHF"
     gene            29247..32183
                     /locus_tag="PKY63_02885"
     CDS             29247..32183
                     /locus_tag="PKY63_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HPE99590.1"
                     /translation="MPHDIEKNLSQLKTHLSGELHFDKAHRIMYATDASAYREFPVAV
                     AYPKDKEDLKTLIRFARENDITIIPRAAGTSLAGQVVGNGLVVDMSKHFTKILEINEK
                     KRWAIVEPGVILDELNRELAKCGLFFGPETSTSSRCNIGGMVGNNSCGAHSLVYGSTR
                     DHLLSVKTLLSDGSEAEFKQLCRDAFREKMTLENLEGNIYRNIREILSDEENQYNIVH
                     EFPDPAIKRRNTGYAIDLLMYAEPFTGGEPFNFCKLIAGSEGTLALMTEIKLNLVPLP
                     PKEKALVCVHCKSLEDSLKANLVALRYHPVSVELMDKAILDLTKENINQNKNRFFVYG
                     EPAAILIVEFAEETQEEIQQKIAALEAAIRAEKLGYHFPVITGSDIKKVWDLRKAGLG
                     VLSNMPGDSKPVPVIEDTAVNPEVLPQYIDEFNALLRKHNLSCVYYAHIGTGELHLRP
                     VLNLRDPKDVMLFHTIALETAKLVKKYKGSLSGEHGDGRLRGEFIPLMIGEKNYTLLT
                     KIKQTWDPFGVFNKGKITDTPPMNTMLRFEPGAQVREIETYYDFSHDHGILRAIEKCN
                     GSGDCRKPFQASGGMCPSYQATLDEQNSTRARANILREYLTNSPKTNPFNHRDIYKAM
                     DTCLGCKLCKSECPSSVDMAKYKTEFLQHWYNSHPVRIRTLAIAYINSINRLALPFSG
                     IYNLFARNYFLSWPIKKIIGFAPQRSLPELHGKSLRRWAVKNLPELNKLTGHNGTVHV
                     FVDEFTDTNDIYIGVKAISLLCMLGYRVELPEHGLSARTFISKGLLKKARKIIDKNIE
                     AISGRISEEQPLIGIEPSAILGFRDEYPELCSTRLQEKAKNIAPYTMLIDEFIVREFE
                     KGKISKIQFTTEKKDILFHGHCQQKALLGTEVTKKMLSIPSHYSVKEIPSGCCGMAGS
                     FGFEKEHYALSMKVGELVLLPSVRNAPEKTIIAASGTSCRHQIKDGTGKIALHPVEIL
                     FEAVKS"
     gene            complement(32362..32865)
                     /locus_tag="PKY63_02890"
     CDS             complement(32362..32865)
                     /locus_tag="PKY63_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99591.1"
                     /translation="MDNTAFSRFLESQKKAFALQNNYSFGTFRADLYGYRRFNMLGRD
                     YYFFHEGNFVNFTSEKCAVMHEAARQHVNAQYKMPKAMRFVVPNIVSIFLSPDGFNEP
                     TLELALQQKRPWQGGEVHDMFFIDTTRKECFGPGYHKVRVEGVEFTLKKTDPTNRSIE
                     LIKELLK"
     gene            32983..34596
                     /locus_tag="PKY63_02895"
     CDS             32983..34596
                     /locus_tag="PKY63_02895"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPE99592.1"
                     /translation="MKDTRYIFVTGGVVSSLGKGIISASLARLLQARGYSVTIQKLDP
                     YINVDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNIRTSQANNVTTGRIYQSVIE
                     KERRGDYLGETVQVIPHITDEIKYRMRLLASHGKYDFIITEIGGTVGDIESLPYIEAV
                     RQLRWELGNHAAVIHLTLVPYLAAAGELKTKPTQHSVKTLLELGVQPDVIVCRSEKHI
                     NESLRNKIALFCNVQPDAVVESVDVETIYEVPLRMRDQQLDTVVMRKLGINESPESNL
                     QVWEDFLFRLKNPVVEVNIALVGKYVELKDAYKSILESFIHAGAANRCKVKVKLVHSE
                     FVNEENIADQLAGMSGILVAPGFGERGIEGKIISVRYARENNIPFLGICLGMQVAVIE
                     FARNVLHLEGAHSREMDRRTPHPVIDIMESQKKVSGKGGTMRLGLYPCKIKAGTKVSE
                     VYNEELINERHRHRYEFNNKYIEMYSMAGMQAAGINPNEELVEIVEIENHPWFVGVQF
                     HPEYRSTVENPHPLFVRFVKAAIDYSEGR"
     gene            34660..36615
                     /gene="yidC"
                     /locus_tag="PKY63_02900"
     CDS             34660..36615
                     /gene="yidC"
                     /locus_tag="PKY63_02900"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPE99593.1"
                     /translation="MKYNQILGFVLIFGILIGFSIWNAPSKEERQKEQARKDSIANVQ
                     QLQDSLNAVAIAQQKDSAKTVTDSAAKQNPIVSAAATQDSVITIDSTSKFTYEKDGFF
                     YTSMDGKDTSYIVETPLMRISIASKGASVKQIELKNFLTWDKKPLEMFYKNNNYFDLS
                     FHTTKSLAKTSSLYFEPIQGFDDNKYTTQINSDSLLISFRAPAMNNDGTEAGALIFNY
                     TFYKDSYLFRFGIAFENRSKFIGSGIQFMEVDWNADLGTKEKSLDNERNVSTVHYADN
                     DLETDYLSETKDDNESIKTSLKWISFKQQFFATSFIADEKFNSADIKSFTRELSDSTY
                     LKTMHSDMNFELNVDKDSAVGFQFYAGPLKYKTLRQYDLALERQIPLGWSFAPLAWIN
                     RYAVIPVFNWLESYALNYGIIILILTILLKIVLFPIALKTYKSSAKMRILKPEVDAIA
                     AKFPKKEDAMKKQQATMDLYKKAGASPLSGCLPMLLQFPILIAMFRFFPASIELRQQP
                     FLWADDLSSYDSILDLGFNIPFYGDHVSLFTLLMTVSTVIYTRLNEKMMGSTNTMPGM
                     RTMMYLMPILFLGFFNSYSSGLSYYYLLANLITFAQIFAIRYFVKEDKVRALIEASKK
                     KPVKKSSWAKRLEEAQKMSAARQTKKK"
     gene            complement(36905..37600)
                     /locus_tag="PKY63_02905"
     CDS             complement(36905..37600)
                     /locus_tag="PKY63_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99594.1"
                     /translation="MKIKDLVFAGIFLVIFVPFFIFPDAFEWFKTMTNEHGVLMSVLK
                     FAVLATLGECLGLRIKTGNYYYKGFGILPRAVVWGFLGMFIFFAFFVFKTGVSAMLEN
                     VFGLEGAVAALKGDFTITKLFTSFFVSAFLNIIFAPMMMTFHKITDSHIEHNGGKLSC
                     LVKCIPMGQIMTDINWKVQWGFVFKKTIPFFWIPAHTITFILPGEFQVLFAALLGVVL
                     GVILAVASQMGKK"
     gene            complement(37606..38274)
                     /locus_tag="PKY63_02910"
     CDS             complement(37606..38274)
                     /locus_tag="PKY63_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99595.1"
                     /translation="MKKLFFFCAAMCTFAFSQSQVNTYNTTGTDLKLTGVMLDDSLNP
                     TQIPRFSLWFNFSEYFHFDVAESFGFFVGIGVENIGYIAQYNDSLNTKVKYRSYLATM
                     PIGFKIGNFDKNNPTFFFAGAAVSVPFHFKEKIFYNDKKDLVFKEWFSSRTNFFQPSA
                     FVGITFPNQMSLKVQYYFENFMNGGFTDNVNGIDIKPYSHIVQSNIIALTLGGSFTGV
                     RKKK"
     gene            complement(38357..40411)
                     /locus_tag="PKY63_02915"
     CDS             complement(38357..40411)
                     /locus_tag="PKY63_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPE99596.1"
                     /translation="MITKELINPQSIVVVGASQDTKKPGGKILKNIIDNHFKGSLYVV
                     NPKESEIQDVKCHAGVKDLPQVDLAILAIAARFCPETVEVLAKQKGTKAFIILSAGFS
                     EENAAGKELEQQVVNIINEAGACLIGPNCVGMMNSNHTSVFTSPIPKLEPKGIDFISG
                     SGATAVFIMESCMMKGVTFGSVWSVGNSAQIGVEEVLEYLDNSFIPGESSLMKVLYIE
                     SVNKPQKLLKHAKSLISKGCRIAAVKAGSSDAGSRAASSHTGAMASSDVAVDALFRKA
                     GIVRCYGREELAAVSSVFSFPKPNGKNVAIVTHAGGPSVMLTDALSSNGLNVPHIDNE
                     ASKELLTKLFAGSSVANPIDFLATGTAQQLDEILNTCENKFNDIDSIAVIFGSPGLFE
                     VFDVYDVLDKHLKTAKKPIFPVLPSIVNAKNEVDYFVEKGNVFFPDEVVFGRALARVM
                     NTPDPLEADGMPAVDTDAIRRIVDHAKDGYLLPEDVSAMLDAAGIPRAGEATSDNEDV
                     LVEAAEKIGYPLVMKVVGPVHKSDVGGVVLNVKTKEKLVEEFRRMMQIKDTTAILIQP
                     MLSGVELFAGASREEGFGHLILSGLGGIFVEVLKDVTSGLTPLGKTEARMMIEGLRGK
                     KILEGVRGQKAVDKDRFAEILTRLSAMLQAAPEIAEMDINPLLGDGSKIVAVDARINI
                     KK"
     gene            40561..41616
                     /locus_tag="PKY63_02920"
     CDS             40561..41616
                     /locus_tag="PKY63_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99597.1"
                     /translation="MRRLMTILLFCGIGWSLQAQTDTIRIPVEDPQKAERLYNEGTVL
                     YDNGQLTPALNKFNEAISIKPDFAKAYYNRGIVKADLKDKTGAMADFNKAIELGPTAN
                     AYFQRGKMKYDDNQPEEAKADLNKAIELDAMHVQSYYYLGSLKYDEKDYEGAIEIYKK
                     ALIDKESYTYAYAYNDMGSCYKMMENYDEAIRCYKEATKANPKLAFAYNNTGSVYRKL
                     EKYDDAIYEYSKAIDADPDYYVAYNNRGLAKMDKGDYEGAIADFTKAIEKNAEYAYAY
                     NNRGSARYKAEQYAAAIEDFDKAITLNGNYSTAYLNRGNAKEMNRDINGACDDWKKAR
                     DGGIKNAEFYIKQMCKQ"
     gene            41638..43674
                     /locus_tag="PKY63_02925"
     CDS             41638..43674
                     /locus_tag="PKY63_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99598.1"
                     /translation="MKNIIISLVCLLVSFTLGAQNVEFNKANFSDKKVLKEALKNIET
                     GDNYFSQGIGARKNALESYLKAQNVNPNNATLNYKIGICYRKINIEKSLEHLQKAETL
                     NPNCDDSLYYWLGIAYQGNYKFNEAIESYKTFKNKLSPKQYSEVAGALNKKIQECETA
                     IELVAKPVKVFIDNLGASINSANREYSPFINADESVLTFTSRRPNTTGGKMDAQDQMY
                     YEDIFIAESAGEDWKVINPGKPLNSKVHDATAGLSGDGQTLFIYKDVRNGDIYECKLK
                     GDKWDKPVALPKTINTKFHESSVSLSPDGRTLYFISDKEGSVGGRDIWVSTLDKKGKW
                     GKAINLKDLNTIYDEESIFMHPDGKTLYFSSQGHKTMGGFDIFKSTFENGKWSEPVNL
                     GYPINTPDDDLFFTIAASGMHAYMMSDTRKDGYGDRDLYKVTFILEKPVLTNSEDNLI
                     AWRTEPVSEKVIESTLAVNTASLTLLKGRVLDEATKQPVEASIILTDLSKNEELATFT
                     SNSATGKYLVSLPSGKNYGIAVKADGYLFHSENFDIPNAAAYQEIEKDIYLKKVEIGK
                     EIVLKNIFFDFNKSTLRPESKNELENLVQLMKENPTLKIEISGHTDNVGSASYNKTLS
                     QARAKAVVDYLIAAGISSSRMTSVGYGFDKPIASNDTDEGRQLNRRTEFKITSK"
     gene            complement(43722..45425)
                     /locus_tag="PKY63_02930"
     CDS             complement(43722..45425)
                     /locus_tag="PKY63_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99599.1"
                     /translation="MLAFARLLSVASFLITTWFCISQFSLMLLLFASLSLIVFLFLMK
                     KQDSIRYRLDLTKALKEINRNEMDFLKSGKSAFDNGSSFLPEKHPNAGDLDLFGEFSL
                     YHHLNRASTTMGNRRLAGKLLGDDSNTDIEAKQLAIKELMPETDLRQMIQATGMLAKD
                     SPEDFRRIVEWSSGAASVLSLVVRTLSFAGPVVLAALIFASFISGAWWIPYAASLVFI
                     FNLLMLGMQMKKLKNEISGADKVDIILKNYSRIAVRIEEKSFQSPMLRNLQNVPGNGS
                     VTISHQLAKLSALFRGLESISNVLALFILNGLMQYHVHALHALLKWKKDHAASLPLWL
                     DRIGEAEALSSLANFAANNTDYAFPEMVAEPGFSFSNLGHPLILHDKRICNDVDFADK
                     RFVILTGSNMSGKSTFLRTLGVNLILARTGAPVCASHARVFPADVLTSIQQSDSLNEN
                     ESYFFAEVKRLKAIMDAASGKPVFILLDEILRGTNSDDKRNGTMEVIRKLAAIDCYGI
                     IATHDLEICNMEPDYPGVLSNMCFEAEIKNNELHFDYKLRGGVCRNKSATFLMKKMGI
                     I"
     gene            45721..45879
                     /gene="rpmH"
                     /locus_tag="PKY63_02935"
     CDS             45721..45879
                     /gene="rpmH"
                     /locus_tag="PKY63_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPE99600.1"
                     /translation="MKRTFQPSNRKRRNKHGFRHRMETANGRKVLARRRAKGRHRLTV
                     SDEKLHKR"
     gene            45956..46906
                     /locus_tag="PKY63_02940"
     CDS             45956..46906
                     /locus_tag="PKY63_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPE99601.1"
                     /translation="MQALTDILFARLLLKHKKIVLQGIGTFTLTETPATAQRMGRDFQ
                     PPKAAVTFSIQEDNDKRLRDILLQDDEFNISMESDAAEICNNYIVGIKFQLEKNGQYS
                     FSGLGNLQITKNGESVFVQDDDSYNYGLPEFTADIVAKQIAAAPVIKPKRKRRVRAWL
                     ILLILIIGGGITSWFLIPEIPENVLPLWEKAISVFDRKEPVTDTTTTKAVDTTQNVMV
                     EPDTIMQDTTQKDTIITEGPLSYFVIAGSFADKTEAETFCNNLKQRGYPEAMVVISEA
                     DRRIRVAYKGFSTRETALDFLNKTKTAENKSDIWLLHQNL"
     gene            46910..47575
                     /gene="trmB"
                     /locus_tag="PKY63_02945"
     CDS             46910..47575
                     /gene="trmB"
                     /locus_tag="PKY63_02945"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPE99602.1"
                     /translation="MAKKKLQHFAENKTFAHYFEMPFVQLSPNGHPMRGQWKNQFFGN
                     GNPLIIEIGCGKGEYTVGQAKHFPNKNFIGVDIKGARIWRGARTIKDEGMKNAAFIRS
                     QAGLLQLWFAPGEVDEIWITFPDPQPGCRQNKRLTSMRYLNMFKAFLNENGIVHLKTD
                     SRELFDYTLEVIAGNNLQLIESIDDLYATPGDGPLHTIQTFYEKMWLEMDKKISYMAF
                     RLI"
     gene            47575..47907
                     /locus_tag="PKY63_02950"
     CDS             47575..47907
                     /locus_tag="PKY63_02950"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPE99603.1"
                     /translation="MEKKNDFFEQVWQVVELIPCGRVTSYGAIARYLGSPQASRTVGY
                     AMNASHASTVHIPAHRVVNRNGLLTGKHWFGGPDVMQQLLENEDIKVIDDKIIDFEKH
                     FWDPTKEL"
BASE COUNT        15168 a        10572 c         9526 g        12898 t
ORIGIN      
        1 atgtggaaat atgagagacg tttgaaagta cgaaatttga aattcgaaat tcgagatgca
       61 ataaaagtga tgatttggtt taaaggttta gaggtttagt ggtttagtgg ccgaccgtgt
      121 cacgttttac gtgaaggcat aaagaaagct acgtagttcc atccgatact cctccgcatc
      181 ctgcggagta aactccggag ttaaatatta atagcccgta tgcctcacgt gaaacgtgac
      241 aagcttcggg cttcggggac gaggaagtag gcattatgag ttatgggtta tgggttataa
      301 gtggaaactc gtaacatcag ctagacacac tgaccttcca tagtccggaa tgcaatgaag
      361 gacatggaag agtcggttgc tgatacctct taccaactat tccatgttct acgtttctct
      421 gcgaactatg gaatgttgga caaagcagaa atccatgctc aattagtttt gtgtggagtt
      481 tatcacgcta aaggcgggac tttgtgcgtt gagctttttg cgccgcgttt atcgggaatg
      541 ttttcagtta atccgcgtta ttccgcgtct aaatcacatg ccgcatgccg caagcgttta
      601 cgtcaccttt ataaacttga tgactttatg aacttttcac ttgtgcagtg ctttgtgcta
      661 ctttttattg taccgcacat aataaatatc cacgcaattg cctccaccaa cgccagggtg
      721 cgcttgcgat gtggaaataa gcagataatt atttttacgc aaggcaagtc caaccggaaa
      781 agaatcagct ttaatctcac ccactttcac catgtctttt gcacgaataa ttacaacagc
      841 acttgcattg ttgcaggccg caaaaatgta cttctcatcg tcgctcactt cgatggtgcg
      901 tgcgccggcg cctgcaaagc actcctgcca ggcagtgaaa ttttctcctt tgttgtcggg
      961 attgatcgca aatgcaataa aatccgcaat ggaacatttt tgcacatatc cgctcctgtt
     1021 gctgctaaga tataaatggt ttttcgtaac aaccaaatga cggacatttg tcctcatgcc
     1081 aaataccgtg cttttaaagc tgaaatcact catgtcgaaa accgaaattc cgcctcctcc
     1141 catacgacca accaacaaat atttgctgtc ggatgtaaaa gccgttccgc gcaaacacaa
     1201 cccacaatgc ttgtcccgat caatgccacc ctgattttgt gaagcagaaa acttttgctg
     1261 ataatcaacc accagatgtt ttacatattt gaatttctcc gcatcgcccg aagacacatc
     1321 aataattgct acagtattgt cgccccaatg ggttattgca acatattttc cgtcgggtga
     1381 tgctgcaatc atttttggca gcgggcctgt cggcataaca cgaacaattt catccttgtc
     1441 agtgtcgata acagccacag cagatggctc catagcattc ggatcgtagc ttcgacgata
     1501 ataagtaacc cacaaatatt ttctgttgtt cgacaagcaa cattcaacgg gtttaccttt
     1561 aaatatattc ggttcttcac gcggagtgag aaaattatat ccaagaaccg ttgtttcttt
     1621 aaacaatttt gcgtcttttt ttgcaaaatc gtgatgaatg cttttgatct ttgtgaatgt
     1681 cttgagatcg aaaaccaccg tcatgaaacc ttcgagactg ttgatgtaga atttgttttt
     1741 ctcttcaaga aataccgctg atttcggaga aataatatct ttttcatagc gttccatatt
     1801 atccatcact ttcggtgtat ggctgtggat gcgcgatatc agcgtcagct ccacctcagc
     1861 tccatcgggg tttgaaatgg ttccaatggg tggatgaatc acctgtgcaa aacaaacgga
     1921 agaaagaaaa acaaatgaca atagaaaaaa cttattcatc agcgaaattt ttagcaaaag
     1981 taagaattcc ttgcagaaac caacgattcc gggtcagcac cttttcaaat gcaggtcagg
     2041 ctgacgccag aatgttgggt ttcctgacat tctatcgtcc gctactgcgg acatggaatc
     2101 gtcgggaatg attcattaaa atacttctgt cgaaataaaa acactgtcat ggatagcata
     2161 tccgcgacag tgacaagtga gttttggatg ttgtttgaaa cgcagcatcc atattgattt
     2221 tcagcgcgtt taacaaaatt gcaaacagcc ggtttgaaaa aaaattctat ctttgttctt
     2281 ctcaaaagtt cagaaaccgg atcttttaac ttcagccggt ttcttttttt caactaaaac
     2341 ccgctcatga agctattttt cggatttctt gtttctttga cattgatatt cggttcttct
     2401 gtttatgcac agaccgatct ggccatgctc tctcatccgg attcagccat cacggaaatt
     2461 aaacctgaca gtgcaaatat ttcacgtccg gcagacagtc ttgcattacg ctcatatatc
     2521 cgcgacagtc ttgtaaacga actcatcact tactctaaaa catttcttgg tgttccatac
     2581 cgctatggcg gaaaatcgcg tgcaggcttc gattgcagcg gttatgtaag caccatgttt
     2641 tcacatcttg gcattacact ctctccaagc tcagtaggga tggccggctc cggacgtata
     2701 gttgcagaca gtaccatacg ccccggcgac attctgtttt ttataaacac acaacgccgg
     2761 cgtagcggca tcggacatgt aggccttgtt gttgaagtta aggatggtaa aattgtattt
     2821 ctgcattcgg cctgcaatgg cggagttcgc tatgattatc tgagcagtcc ctactacaag
     2881 tcgcattact acagggctga acgcattcca ttgtttgacg ttgaatagtt ttatcagcgc
     2941 acatggaact cattaaaaaa tacttttccg gtcttactga aacgcaattg tctcaatttg
     3001 cacaattgga gccgctttac aacgactgga acagcaaaat caatgtcata tcgcgcaagg
     3061 acatggagca tttttatctg catcatgtat tgcattcgct ggccattgca aaggtttttc
     3121 ctttcactcc gggaatgcgc attctcgatg tcggcacggg tggcggtttt ccgggcatac
     3181 cgctcgcaat atttttcccg gaagtacaat tcacgcttgt tgattccatt gggaaaaaaa
     3241 tcaaagttgt aaaagaagtc gcttcggcgc tggaattgaa aaatgttgaa gccactcagg
     3301 cacgcgccga agaaatgaaa ggtcagtggg atattgttgt cagccgcgcg gtaacggatc
     3361 tgccaaaatt catggagctg gtgcgcaacc gtatcacccg aaaacccaaa ggaaatcaac
     3421 ccaagattat ttatctcaag ggcggagata tcgatgcaga gatgtccgga tccgattatc
     3481 cctacagcgt tgcacctatt tcagggtttt tcagcgaaga cttcttttca accaaattgg
     3541 tggtcaagat tttttaatct acgacaatta tcatatcatc aacaattttt aaccgcttgt
     3601 ggttaaaaaa caaagttctg cgtatttaat tttgttgata acattcacac attgtctcaa
     3661 ctttttacgt tgttgcattg gtatttttaa taatttggcg cctcaaatta aaactacaac
     3721 aatggttgaa aatgcacaaa aaacgctgag agacaatccg gcaatccggt ggacagcttt
     3781 ggtattactg gcttttgcca tgttttgttc atacattttt atggacattc tttcacctat
     3841 taaggacctg atgcaatcca cccgtggctg ggattctact gcatttggga ctatgcaggg
     3901 ttcggaaaca tttttaaatg tctttatttt ctttttgatt tttgcaggaa ttattctgga
     3961 taaaatgggc gttaggttca ccgctatttt atcgggattg gtcatgataa tcggggctgc
     4021 cataaactgg tatgctgtta ccgaaaactt catgggcagc agtcttgacg tatggtttac
     4081 caacaatctg aattacattc ccggcttcaa cgaattagga attgctccgt tttattcggg
     4141 catgcccgca tcggcaaaac tttccgccgt tggtttcatg attttcggtt gcggtgttga
     4201 aatggccggt atcacagtat cgcgcggaat cgtaaagtgg ttcaaaggca gagaacttgc
     4261 attggccatg ggctctgaga tggcactagc tcgtcttggt gttgcaactg taatgatttt
     4321 ctctccgtta tttgccaacc tgggtggtaa tgtcgatgtt tcgcgctcag tggctttcgg
     4381 cgtcattctg ctcatgattg cccttatcat gttcattgta tatttcttcc tcgataaaaa
     4441 actggatgca caaacccagg aatcagaaga aaaagatgaa ccttttaaaa tcaaggatat
     4501 cggcaaaatt ttctcaagca tgggattctg gcttgtagca ttgctttgcg ttctttatta
     4561 ttcagccatt ttccctttcc aaaaatatgc tgtaaacatg cttcaatgca atctcacttt
     4621 taccgaagtg gagaaaggaa cattctgggc atcgaataac gtaacaatgc ttcagtatct
     4681 gattatgctt attgtggcag ggactgcctt tataagcaac ttttcaaaaa aaacaatgca
     4741 ttattccatg ctgactatat ctgttattgg cctcatcgtt ttctgctaca tgggttatat
     4801 gcgccaatcg gcagaaacca tttttgctgt atttccactg ctggccgtag gcatcacccc
     4861 cattttggga aaatatgttg acaataaagg gaaagcagca tccatgctta tgattggatc
     4921 gctattactc attgcatgtc acctgacttt tgcattcatt ctgcccatgt ttcagggcaa
     4981 tgccattggt ggggtaatga ttgcatatct tactattctg attctgggag catcattctc
     5041 attggttccc gcatcattgt ggcccagcgt tccaaaactg gtggatcaga aagtcattgg
     5101 ttctgcctat gcactgattt tctggattca gaatatcgga ttatggctct tcccgatgct
     5161 gatcggaaaa atccttgaca acacaaaccc acaggttgtt gctgatcttg aagccaaaat
     5221 tatcacaccg gaacaggccg ccatatccta tgattacacg gcgccactga tcatgcttgc
     5281 cggtcttggc gttgcagcac tcattcttgg tattattctg aaaagagttg accgcatcaa
     5341 aggactgggc cttgaattgc caaatattgt aaaaaaagac taaacagtta ataactgaaa
     5401 tatccgcaaa cggctgaatg gacttcattc agccgttttt ttatttataa gcgcctgttt
     5461 ataaaacaat caataacacg aaataataca aagaattatt ttgtactttt accacaaatt
     5521 ccgcatctat gaaaacaaaa aacttattgc ttataactgt tgtttttgca gcatttcttg
     5581 tatcttgtgt tcctgcacgc cagttacagg atgaaaaaac acgacgcgaa aaagctgaat
     5641 cagaactggc tgccatgcgt gccgatttcg ataaaatgag tgcaaccaac acggagctca
     5701 acgaaaatat gaccaccatg accaaacaaa ttgccgcttt ggaacgcgac accagtatca
     5761 ccggcatatc gctgcgcaaa atgaccaatc aatacgataa agtaaacagt ctttacgaag
     5821 aactcaaccg caaatacgaa gaacttctta aaataaatgc atcggaaaca caaaaaataa
     5881 gtactgaact acaactcact caggaacaac ttgccatcaa agaagccgaa ctaaaaaaac
     5941 ttgaaaacga actcaatatc cgcaaacaaa atctcgatca gatgcaggcc gatttgttgc
     6001 tcaaagaaaa aagaattatg gagctcgaat ccatcatcaa caaaaaagac agcatcatga
     6061 aagagctgcg caacaagctc acagtcgccc tcaaaggatt cgaaaacaac ggcctgacca
     6121 ttgttgaaaa agatgggaaa atttatgttt ctctcgacga atcattgctt tttgcatccg
     6181 gcagctggac tgtaaatgca aaagggaaag atgccctgat gaagctttcg aatgtactgg
     6241 ctgataatcc cgacattgcc atcatggttg aaggccatac cgacaatctt gcatataaag
     6301 gcagcggaca agtcaaggac aactgggatc tgagcgtgat gcgtgccacc agtgtgacca
     6361 aaatcatttt aaacaataaa agtgtagatc caaaacgcat tatatcagcc ggacgcggcg
     6421 aatacttccc gattgacgga gccaacaaca gcgaagcccg ccagaaaaac cgacgcaccg
     6481 aaatcattct gactccgaat atggatgaat tcatgaaaat tctacagaat aattagtact
     6541 attctgtttc tcgcttttca acaaaaaagt ctcagaatta caaatattct gagacttttt
     6601 ttatacaaaa aaaccccggc gataaaaaac caacagacat aattatacaa caaatacatt
     6661 aaataaatac acatgagaca ggattaaaca gactaaatat tatcaataca ttttaaaaaa
     6721 aaaagtattt ttttgagttt tattatcagg aataaaaaat ttatatcttt accaaaacat
     6781 ttttatatga aacatttctg cataatttcg attgtatgtt tccttatgaa tgcaggttta
     6841 caggcacaag aagaatacat acagccccgc aaacacctgt tggatggaat tgagcaacac
     6901 gacaagggcg aattttccaa agcgttgaaa gcgtataaca aaatcaactg caacgactcg
     6961 ttgtatccat tggcactgta tgaaaaatcg ctgagttata acgctctgga agaatatgac
     7021 aaagcgatag aagtattaaa tgaattacta agcaaatccg acagcaccag tcagattctc
     7081 atttcactgg cctctgccta tgacgataaa aaaaaccatg ctgaagcaca ggcgatatac
     7141 aagaaagtca ttgaccggca gccctacaat cagaatgcct ggtataatct cgggctgagc
     7201 tattacaatg acaagcaata cctcaaagca gaagaatgtt tcattcaatc catcaaactg
     7261 aaaccatggc atttcagatc gaatctgata ttgggcctta ccaatgccaa gatgggacgc
     7321 ccggttcatg cactggcttg cattaatctg gccgctttaa tgaatgccgg cgacaatttc
     7381 ggccattcgg cattgcagga atatgaaaaa tttgttcatg gtgattatga agccgatatt
     7441 gaatacgaca aatatgttac tccggatagc ataaccaccg ataaaatcag agcattggaa
     7501 cccatcatca aaagtcaggt gctgcaaagc aaaaagttga aaatgccttc gaaaaacccc
     7561 atgactatta caaaatacaa ttatgtaatt ttttcggaac tgccacaaac caaagaaaaa
     7621 aacaacatct acactgctta ttacttcccg tttctgagat cgatactcga taataaatat
     7681 tttgatgttt acagttattt cattactcaa tcggtcaaca ataaaaacat cattaaatat
     7741 gttctgacaa aaaaatcgaa actaaatgcc ttcagcggct ggatgatcaa agaactacaa
     7801 aaatatttcg ccagcccgtt tttcagacca tccaatgttg attatgcata tgccgaggga
     7861 aatctgattc ggattaccac ctacagtgat tccacaaaaa cagtaaaaga cggcaaatat
     7921 atcgagcttg acgaaaacgg agatcttgaa tacaccggaa catttaaaaa cgacaaacac
     7981 gaagggacat tcaatagtta cagcgttgac ggcaaagtaa gagtatttgt caaatacaag
     8041 aacgatgatg aatacctgct tgtgtcaaat cctgaagtca ttaagggaaa tcagttcgaa
     8101 gttgaaatga aagacggcaa aaagaatggc tggtataaaa cattctttcc aaacggagct
     8161 gtcaaatcaa tcgaacttta taaagatgat gaatttgttg gcgatggatt tgaattatac
     8221 atagatggtg acaccatgac aacctacaag cccgatgccg acggacttca ttacacaaca
     8281 acaaattatt accgcaacgg gaaaatcagc acctgttaca aaagattgat tgattcttac
     8341 ctgtacgacg gagaatatat ttcaaacttt tataacggga aactggcacg aaaaatcaca
     8401 ttgaaagatg atctttttat tggaaaaaca tttacacttt ttctcaatgg agatactgca
     8461 gccattcaga aatatgatga caaaggaaat gccacaggca ttcacagctc cttcggaaca
     8521 tcaggtaaat tgatttcttc gatagaatac cagaaaggca aaagcaatta tatcttcact
     8581 tattaccacc gcaacggaaa acctcagtcg cagctgaaag tgagcaacaa taaattcaat
     8641 acgctggtat attacgacag caccggaaaa gaaattgtgc atcatgataa tctgaaccgt
     8701 aatctgaatg aatacacaaa cgtcaatgta tttacaaaaa aaatcattga tcatttcgta
     8761 ctcaaagacg gattgaggga tggtccggca aaacattaca cacactccgg cacacttgaa
     8821 agcgaatgca aatacagcaa agatgaaatt gacggagtca atgtaaccta ttatcccgat
     8881 ggcacaaaaa gaagtgaata tacatacgaa gaaggcgttt tgaaaggaaa agccatcagt
     8941 tattattccg acggcaaaat tgaaagcgaa ggattttatg acgacgacaa caaagttgga
     9001 gaatggcgct actttcatgc tgatggtaaa ttaagcaata cagaatacta caacacatca
     9061 ggcgagctca acggcgaaca aattgaatat tgtggagaca gcatccccgt atccgtattc
     9121 acctatcgcg aaggcaatat cattgatctt gttttctatg gtcccgacat ggatacgctg
     9181 gaacattact ccctgcccaa cggattcgga aaaaacacca tttattacaa aaacggaaag
     9241 ctcaaaacaa cacgcaatta cgagcaccat gttctgcatg attccgttgt ttattattat
     9301 ccaaacggac aactgtatta caaaatcaat tatgtcaacg gattcagaca tggcagatat
     9361 tgcgaattca acccggatgg aagcaaaaaa agagaacttg attttgaata cggacaacgt
     9421 tgcaacgaaa acaatttcta cctcgaaaac ggcttcccgg atgcttcgct gatttgcaaa
     9481 aacaatgaag aaaatggtcc ggccacttat tatcactaca acggaaagcc aacagccatc
     9541 atcaatttca gcaatgatga aaaaaacgga tttgccgact attatgaacc cggcggacaa
     9601 ctgatgtacc gtgtatggta tgaatccaac gaagccgttt ctgttacatg gctcggaaaa
     9661 gacaagaaat ttctggaacc ggtactcatt gacaaagata ccatcaatat aaccacgtat
     9721 tatgcttcag gcagcaaatc agctgagctt tcatttgttg gtggcatgcg gcatggcgaa
     9781 ttccgcggat atttccccaa cgggaaactt ctcgaaataa gacctttcaa aaacaattat
     9841 ctcgatggaa aggaaatctg ctatcgtgat aatggtattt tatttgccga aaccgaatat
     9901 ctcatcggcg ataaacacgg agtttgcact gtttatcatc caaatggcaa accggctgtc
     9961 agaactgaat attatttcgg agaacgcaaa gggacacaaa cctattacaa cgacaaggga
    10021 caggttaaaa acgagtatca attctatggc gacagaatgc ttgaatagaa caattctcac
    10081 atggttatgc ctgattgctt tgtcattggg cgatagtttc ggccaaaccg aatacgacta
    10141 tgaatatctg aaggaaaagt attcttcgga aaacaccgtt gtgctcactt cgcgcaaaga
    10201 aatttcggtc atccgcaaaa aaaagaaaat tgtcattgtc gaaaaagttt ttctcgaaaa
    10261 actgatgacc ggaaaaaaca aggatgaagc ccaactggat attattccct tcagcacatt
    10321 tgcatctatc agtagcattg aagcatacac actggttccc gataaaaacg gatggaaaaa
    10381 acatatggcc tggaacttca gagacatgct tcatgtaggg tcttatctct acaacgacaa
    10441 ttatctgaaa ataatcaaat tcccggctgt tgaacaaggg tgcagaacca ttatatccta
    10501 taccaaaaca tacacagagc cctatttgtg gggagggcat tttctctcca atggtgaaac
    10561 cgtactcaat ggtgaaatca ctttaaaagc tcccaaaagc atcacattca atatcaaaac
    10621 gcacaacttc cagcaggata gtctgttgtt tgaacttgaa tcaaaaggat cgtggaatat
    10681 gttcaactgg caatacaaaa ccgaaaagac caaaaagccc gacgatcagc ccctgttttt
    10741 ttccggcccg atgcttttga tgaatattgc ccgcgacacc gataaaaagc acggacagta
    10801 ctatcttgaa agcaacaaat atcttgcaaa ctggtatttt cacaattttc tgaaagcaaa
    10861 aaaccaacgc gacaccgtga tggaagaaat tgccgacagc atcacaaacg gtctgaacta
    10921 taccggtgaa attgccatga caatgaccaa ttgggtaagc aaaaacatta attacattgc
    10981 catcgaagac ggctacaacg gaatcatacc ccgcagcagc aattatgttt taaagcaacg
    11041 atacggcgac tgcaaagaca aatcgaacct gctgattgaa atgttgaagc ataaaaacat
    11101 ccgggcatat cctgtttttg taggaactac ttcattgcca ttcagccacg acagcattgt
    11161 atcacccatt gtggacaatc atgtgattgt agccatcgat acgggaaaaa acaacatatt
    11221 gtttgtcgat ccaacacagg aatatcagat tgaaggactc ccgccttttc ttgttcaggg
    11281 caaaacagcg ctggtactta ccgacagcat ggactatttt attgagcatt tcccaacacc
    11341 cgatccggag cttagcaaat tgaccatgac aaacgaaatt ttcattgaag gtcccacact
    11401 ttttcagcaa accgatctga cagccaatgg atacttcatg gtttttataa atccaatgct
    11461 gacagacaga ccagaagaca agattaaagg cgactgcgag ttcattagtt ttccggaatc
    11521 aaaaacacag attcccgaca gtctgagtga tttggagctg aatcccgact cggcatttgt
    11581 atcgttcact attttttcgc atcaggcaaa tccgctacaa aaaacaaatt cgggtatttt
    11641 catcaatcct tatcccaacg ggtttttcac gctcaaatca tttcaggagc tggaagaaaa
    11701 aaaacaaagt gtatttcatt ttcctgccac atgggaaagc attactacac tgcatctgga
    11761 aaatgattat catgtaaaat accttcctca aaacatattg attgattcgt tgcattactc
    11821 cttttcattg gatcacagca aagaagaatc cgatgttgtt atccattgta tcctacgcat
    11881 caacaaactc tattttactg attcagagct cgatgggctg gcctcattct ttgctgatat
    11941 acgcagagca tttaaccaaa acataataat cacaccaaaa aattaaatca tgaaactgtt
    12001 gatttttttc attgctgttg caggcgtttt gattgcttcg gctcaaagac ccgaaaataa
    12061 gtcatactca tgggaagcca cgcctcaatt tgtgcaatcc gatagtgtag aagcaaaaca
    12121 tgatgccgtg tatctcaaaa caaaaattat ttatgatttt tattttgaaa aagaatcagg
    12181 agatctggcc gaactatttg tttttcacag agcatataaa atctgcacac aaaacattac
    12241 cgaagatctg aacaccataa aagtgagcac cagtgatttc aacaacatgc ttgacctgaa
    12301 agtgcgggtt attgcaccgg atggcaaaat caccgatgtt gacaccagcg aatttcttac
    12361 attgaacgat gaagtgaaca acggagatta caaagtgctg gccctgccaa acgtttcgcg
    12421 tggcagcatt gtggagtttt attatgtatt ggagtcctct cccacgtatt ttatctcacg
    12481 ctctttccaa tacggcattc cgtcttttga tgtgtcattc aaagtgtact cacccatcaa
    12541 tctcggtttt atctcaaaat cctacaatgg ctttccggaa cttgaagaaa aaacagacag
    12601 caccgaagaa taccgggtaa tgtcggcaca cactgattat atcccggcat ttcaaaaaca
    12661 aggatacagc tattcgtcgc ctcatgaaca acgcgttctc atgtcgatcg gatataattt
    12721 tgcacgcagc aaagcccgtc ttttcacaca tgccgaattt tgcggaaatc tttatgaaag
    12781 actatttgtt ttagaaaaaa aggaacaaaa agcattgagc aaaattgtca aaaaagcaaa
    12841 aagcaaagat cccgacaaat taattgcagc acgcgaaatc gagaattatc tgaaagccac
    12901 ttattttaat gaactgaaca tcggaactgt tacagaaaca cttgatttta ttgaatcaac
    12961 ccgcttcgga tctgatatcg gcattgtaaa attatacatg aatatttata aaacactggg
    13021 atataaatgc aatttactgc ttacatgcac aagagattac atgtacttcg acaaatcctt
    13081 caacacctac aaccaactga atgattatgt actctacttt cctgaattcg acctgtacat
    13141 gtcgccgctg catttcacat ctcgattggg tttccccgac aatctggtct atgaacagga
    13201 tgctttgttt attaaagaaa cgaccattct gggtgaaaca tcatttgttc cagactacac
    13261 aaccataaaa aaacccgatt acaaaagcag tgctgattct gtctatcaga ttctgaccat
    13321 taatcctgat tgcaaaaccg cttcactcaa atgccatcgc tcactaacag gacacaaagc
    13381 atcctatatt cagggaatat attcctatat ggatgatgaa gaaagagaag aattgctgaa
    13441 agaatatttt gattatggcc atgtatattt caatcccgat tctgccataa ttgccgatac
    13501 ttcaaaaaaa gtggccggaa tcaaaccatt taccatcgat gccaacctga atggcagcaa
    13561 tctgctttcg caggccgacg atcatttgat tgtgcttgca ggcgaactca tcggaccaca
    13621 ggccgagctc tacgacagcg ttcaacgcac actccccatc gatcacggct atggatgtca
    13681 atacatcaga acactggaaa ttgttattcc ggaaggatac actgttgaaa acgctgctga
    13741 tttcgatatt aatctgaagc ttaattcaaa aaccggagaa caaactgcat ggtttatttc
    13801 caaacaacgg ctcgaaggga acaccttcat tattgatatt gacgaatcat acactgagat
    13861 caaatacgat ctggccgatt atgagcaatt cagaaacatt gtaaatgctg ctgccaattt
    13921 caataaaata aaattagtcc tgaaaaaaag ctgataagtc tcttttcatg ttacagatac
    13981 tccataaatg acatggattt taaaaagggc gtgcattgtt tgcatgccct ttttcatgct
    14041 tcagggacat cgcatgcaaa cgagaaccca aagcccagtc ttaaccgcgc ccacggcttt
    14101 ttatgttgcc gaacagttat ctgaaagaga tttattaatt tagcacaaaa aaatggactc
    14161 acgcccactg gcagaaatat tgcgacctct gaccaccgat gattacgtcg gacaggaaaa
    14221 actcatgggc gccaatggca tcatccggcg catgcttgac aacaaagcca tcagctcgat
    14281 gattctgtgg ggaccaccgg gcgttggtaa aaccacgctg gcattgctga ttgcaaacgt
    14341 tctgcagcgc aaataccatc agctcagtgc catcagcagc ggagtgaagg atgtgcgcga
    14401 agtgatatcg gcagcgacca gtgaacaaag tcctattctg tttatagatg aaattcatcg
    14461 cttcagcaaa tcgcagcagg acagtctgct ggcagcagtt gaaaagggca caataacact
    14521 aataggagca acaaccgaaa atccctcatt cgaagttatc agcccactgc tgtcgcgctg
    14581 tcaggtgtat gtacttgagt cgctttcatc agatcacctg aacatacttc tcgaaaaagc
    14641 acttcaattc tataaaaaag aaggatacac catttcagtc aaagaaacag atatgttgtt
    14701 tcgtttttca ggtggcgatg cgcgtaaact ctacaatgca ttgcagctgg ttgttgaaaa
    14761 cagtctgaaa aacaattcgg ccgaaatcac caacgacaaa gtggcacgcg taattcagaa
    14821 taatctggcg cgctttgaca aaactggaga aatgcactac gacatcattt cggccttcat
    14881 aaaatcgatg cgcggcagcg accccaatgc cgcattatat tggatggcgc gcctgattga
    14941 aggcggcgaa gatccaaagt ttattgcccg ccgcatgctc attcttgcct ccgaagatat
    15001 cggtctggcc aatcccaatg ctattctcat ggccaatgct tgctttgatg ctgtacataa
    15061 aatcggctgg cccgagtcgc ggatcattct ggccgaatgc gcagtctatc tggcttcgtc
    15121 ggcgaaaagc aatgcaacgt atcttggcat tgatgcggct cttgagcatg tgagaaaaac
    15181 cggtgatctt ccggtgccgc tccatctgcg caatgcgccc accaaactga tgaaagagct
    15241 gaactacgga aaagaatatc aatatgcaca cgactttgaa aacagttttg tgaatatgga
    15301 gtttttacct gaaaaaataa aagggacaaa attctacgaa cctggaaaaa ctgcacggga
    15361 agaggatttg aggacttttt taaaaatccg ttggaaagat aaatatggat attgattgtg
    15421 ctgtatgcaa cattttatgt tttgtccgtt attcaacaca aatactccat atcagaaaaa
    15481 acaattaggt ttgtacatta aagtccagaa cgatctatga ttctatcagt catcataccg
    15541 gcctacaacg aagaaaagta cattgctgaa acactttcag aagtgtatgg cctttcattt
    15601 ccggccatta tcgagaaggt tgaagtgatt gttgttgatg attgctcaaa agatggtaca
    15661 acaaatattc tgaatgcaca aaaaacaaaa catccggcat tgatagtgca tcgcaatgaa
    15721 aaaaacatgg gcaaaggcgc ttcggtcaaa aaaggatttg aaatcagcag gggtgatttg
    15781 atttttatac aggatgctga ttctgaatta tcgcccggcg acatcccccg gatgattgat
    15841 gcgatgcttg aactgaaaat tgaatttgtc aatgggtcgc gttacctgcc cgggattgtg
    15901 cgtcctgtaa tcagctacaa acgttatttt gccaaccgtt ttttcacctt tctggtttca
    15961 tttctttcaa atgccaaact tactgacatc gcatgcgggc ataaacttat acaccgcaag
    16021 ctgcttgaaa aaatccccat acgatcgaag cgtttcggat tcgaagccga aatcatgatc
    16081 aaagctttaa aaataaaaaa gaacaacatc gccgaagttc ctgttatgta ccgtccgcgc
    16141 agtgtgcgtg aaggcaaaaa actgaaatca tccgacggca tcaaaatcct ttttacaatt
    16201 ctgaagtatg aatttttctg gtgtcctgca gcaaaaaaca agtcatagca aaattccggg
    16261 tctgataatt attgccctgg tattgttttc ggccgtattt gcccacgtcg aaaaaagagc
    16321 tcataaaagt gtcatcagcg acgacgttat ctcatactat gcttatttgc cggcattgtt
    16381 tattcatcac gatctcacac ttgaatttgc caatgaggat cctgcatatt atttcaaata
    16441 ttactggcct gggaaaacat ccaatggcaa atatgtaata aaaaccacca tgggcgttgc
    16501 ttttttatat gctccgtttt ttgggacagc gcatttatac acacttgcaa ccaacggcac
    16561 tgccgatgga ttcagtcggc attatcaaac cgccataaat atagctgcct tgttttttct
    16621 ctttatagga ttgtttttca ccaacaaaat cctgctgcga tttgtgtcgg caaaagtttc
    16681 tgctgttgtt cttgttttga ttacacttgg aacaaatctg ctgtactacg caacaatgga
    16741 gcccggaatg acgcatgttt tcaatttcgg actcattgca atgtttattc attattcaga
    16801 taaatggctg ctgaagcccg atttcagaaa cacacttttg tgcggactgc tctttggtct
    16861 cattacactt atacgaccaa tcaatgcact tgtcatattg tttccgtttc tgtggaatgt
    16921 gtcatcactg aaagaattgc aaaaccgctt gttgtttttc atcaaacaat ggcaactgat
    16981 attactgatt gccgggtgtg tgctgctggt ttggatgcct caactgattt actggaaaat
    17041 acaaacagga caggtgtttt acaattcgta caacgaacat ttttattttc acaatttcca
    17101 tattcataaa gcattattcg gattccgaaa aggttggttt gtatatacac ccatcatgtt
    17161 tattgcctca ttgggcatat tgtttttacg ccgacaattc aaaccggttc agctttcagt
    17221 tgctgttttt tttatggttt ttgtctatat cactttttca tggtggtgct ggtggtatgg
    17281 aggcggcttt tcggctcgac caatgactga tatttattca atcatggcca tacctctttc
    17341 agtcttcgtt gcaaaaggtc ttcgtgcaaa aaaatcaata gcgataatca cttttttatt
    17401 tacaacatta ttcgctgctc atggggtata tcaaacattg cagtatgtta gaggaagcat
    17461 tcattatgat tccaatacat ggcattcata taaaaattcg ttcggaagga tcaaacccgg
    17521 gccaaactat tattatctgt tgagtccccc tgattacatc aatgccattg agggaaaacc
    17581 ggaggaaaca gcagaagaaa acaccgatct gattgccgga ccaaatgatt actatattga
    17641 aatgaatgat attagaacca gcaacaatac tgaatatttc aacatcgtaa acattaatct
    17701 tgatacaagt tttataacca cagcaacgat tgtgataccg gcattcgata gcaatgattt
    17761 ctgcgatgac ctcactttgg taactgccat ttacacgcca tcgggcgaag cagcatattt
    17821 cagctcagtt gctgttgcag ctacaaccag atatggagat aaacaattgg ttgaaaatgt
    17881 cgaattgaat attcctttat atgttactga aaaacccaac attaaggtgt ttttcagatc
    17941 cattgaaaaa gatgattttt catgcaaaaa agtgaaagtg ctgattcgtg gaatacggct
    18001 ccattacacg gatgtataat agtccaacca cattatttcc caccaaaaaa aacatagagg
    18061 cgctcaataa agccccagcc gaaatccggc aatgtcgctt ctgtagccat aataataaac
    18121 acatcgtgat cagccacaga ctgagccaga tcgagctgtg atgcttcaag ctgattggtg
    18181 taatattcag gataaacccc cttgttgtaa taccagaact gattattccc aaacgatcgc
    18241 ggaaaaccat aattgaacat ttcccaataa taactgtcgg atacaacaat cagagaaggt
    18301 ctgctttttc cggtagtatc ttcggtatag agctttggat aagccatgtc aaaagatccg
    18361 attctgaata acatattcat gccatcgcca atatcataat cttcgaattt cggctgacaa
    18421 acctcaatgg aatcccaacg cagatcgggc atatcgatat ttcgctttct ttcaataaaa
    18481 cttatcatgg aatccgctgc cagacagctt ccataatagc tccagtgaat gccacatttc
    18541 ggataaagag gatattgcga cgaatgttta ttttccaaaa aatacgaatt aaaatcgata
    18601 taattcaaat tcagatattc tgccagatga ctatgaagct gataatttga aattcctttt
    18661 ctttgtttta aatcatccgg gaaatattcc ggataaaaag atcccttgcc ggcagcaaag
    18721 actataagca atgtttttcc gcgatcagcc agggcattct gaacaaactt aatgcgtttc
    18781 attctgcgta cagtgctatc aacgccaata aaatcggtac cattgtacgc tttgatataa
    18841 ttctcttcaa ataaataatt gtctttcccg ataatcacac cattggcttt ggctttacta
    18901 aaaaacgaaa atgctatctg attgttgagc cgaacaaaca cgcttcgaag cccaaacgcc
    18961 tcgttgagat aggtttcctt ctgcacctga tattcaccag aaaaccagcc atgggttgaa
    19021 aattccggac ctacgggctc attaacagcg ccacgcaaag gagtggcgtc aataactttc
    19081 aatttattat tgataaaagg gcctgtcatc aagagaaaca caccggccac caataaatat
    19141 ttcactattt cgtctcttcg ttttttcatg tcagaatcta aaataaatga acgggttgaa
    19201 ccccgacgcc gttatagaac tgatgcaaag aataaataaa acaaacactg ctgcagctac
    19261 tgaaagatgt tttttcacgg aataggtatt gaaataaatg gcattctgta tttgctcacc
    19321 aactttaaaa aaagtaaaga acgcaaagaa tacagcgaga aacagaaaaa ccacaaactc
    19381 aacatcaagt accggaagtg ttgtaaaatc aagggcgaac atgcgattca gaaacagaaa
    19441 agcttctgaa acattttcaa ttctgaagaa aacccaaccg atgacgacaa gaaaaaacgt
    19501 gagcaaggtt gccggaagtt ttccgatgcg ttcatatact ttaagcaaaa agccacgttc
    19561 gagcacgaga aacaatccat gataagcgcc ccagataaca aaactccacg atgcaccatg
    19621 ccataatccg gatgccagaa aaacaaacca aagattaaaa tataatctac gctgcgtttt
    19681 cacacggttt ccacccagcg gaatataaag ataattacgc atccaggcac ctaacgaaat
    19741 atgccatcgt cgccagaact cagtgatact gcgcgacaca taggggttat tgaagttttc
    19801 cggaaaacga aagccaatca ttttcgcaat accgatggcc atatcggagt aaccggaaaa
    19861 atcgaaataa atctgaaatg tgtaagccag aataccaatc cacgctgttg cggtatcaag
    19921 ccccgaataa tccatcgcaa aaatcatgtc ggcctgctgc cccatatgat tcgcaataag
    19981 cacttttttc gccaacccga tggcgaaacg ataaaaaccc gtcagaacat tgtctttcgt
    20041 ttcatactcc gagcgatcgc ctatctgatc agccagatca tgataacgaa taatgggacc
    20101 agcaataagt ttggggaata aaataatata gagctgataa tcccagaaat tcttcaatgg
    20161 cgcatgaaca cgacgatata catcaaccac ataggtgatg gtttcgaatg tgtaaaacga
    20221 tattccaatt ggcaaaatca atttggtcca ttgaatctca cctccgccga aaaccgacaa
    20281 agccgcattg acattttcaa taaaaaaatt ggagtattta aaataaaaaa gcaaacctaa
    20341 attcactgaa accgacaacg taagcatcaa tttgcgatgc agatcattct ttgtctgcga
    20401 catccatttc acaagatgaa agtcgagaaa ggtggtgcca agaatgacaa aaataaattt
    20461 tggtgcgccc caactataga aaaaaatact aaaggcaagg atgacaaaat tcttgtattt
    20521 tttgtctgca aagtagtatg tcagcagaaa tgccggcaga aaatagagaa gaaagactat
    20581 gctgctgaag accatcttgg atgcaaagtt attgattttt tcacatgcag tcgttacagt
    20641 cgtggaattt atcacgcgga aagcatggcg ttacaatcgt tacatggtgg ctttaacaca
    20701 aatagcatcc tgtgcaacat ctaattgatt atctttgtct tcttgaaaag agaaaaatga
    20761 agtcggtcag acatatcatc atcttggctt tcatggcagg attgctggca tcttgtgcca
    20821 aagatccttc gtgggacgta gatgtgcgca ctcctctttt caatacctca cttggagtga
    20881 atgacttaat tgccgacagc atgctgatag aaaatcctga cacaagtctg acttttgtca
    20941 tggattttga tttattcaac atcactgccg acacatttgt aaacatgcct gattcgcttt
    21001 tttacgccca ttatgtcatg cctgtaggat tgactgtgcc acccggaaca ctggtacttc
    21061 aaaaaacaga aagcaggtat tacaatcttg ggggtgcact tttaacccaa atgcgcatta
    21121 aaagcggcac actaaacatc agggcttata attacgtcgg cgatgcggcc tatatcgaat
    21181 actccataga taactcaatg ctcaacggag ctcccattgt agcgagcggt ctgatcccaa
    21241 gtttccctgc aactggagaa tatctggaaa cccagcttga tgtcagtggc atgtatctcg
    21301 acctcactca gtcatacatg cattgcaatt cgctcaaatc aacaatgaaa gtattcgcca
    21361 atcccaatgc aacaactgat atagtagtga atttcaatga cagcatcaat attctggttg
    21421 aattcaaaaa tatcgtcatt gattattccc gcggatattt tgggcaacat gagattaatt
    21481 ccatttctgc tgaaagtttt ccattcatcg aagatcttgg cattgattat ctcgacatgg
    21541 aaaacattgc aatgacattg actatggaaa accatctggg tgctgatgca acattcacaa
    21601 tcaatgaact ggctggtcac ggaattacag atatcgcact gaattcggaa tggattggaa
    21661 aagcaatcaa ccttacaagg gctacagaaa accctgtcaa ttcgggaaat gtaacacctt
    21721 ctgttttcga aatgaacttc gatgcatcga atgttgaagc tttttttgaa aatcttccag
    21781 acagcatttc ctatgatttc agcggcacag taaatccgct tggaaatatt tctaccggca
    21841 atgattttgt ttattacgga caaggacttc gcggaaagat acatgccgaa ataccgatga
    21901 atattgctgt tcagggatta acacttagcg acaccattga ttacgaatta aataaatcaa
    21961 acaatttcat cactcagtcc gaactgtttc tcgatatcag caacggcttt ccattgacag
    22021 cttcatttaa aatatttctc accgacgaag ctgacaatgt tattgacagc attattcccg
    22081 acaacaatat accctcaggc acagtcaatg ctgaaggaaa agtaacagct ccatcgctga
    22141 ctcaaatgaa aattacactg aatgaaaacc agacaacgca cctttatgca tgtaaaaaag
    22201 ccattgttgt tgccaaactc aacaccggag gaatcagtgg tcagaaagta aagttttatc
    22261 catggtatca tttgaatgta cttgctaccg gattgtttaa tatttttatt gaatgaaaag
    22321 tacattcgtt ttttttctta ttctaatgac gtatttttta gctgctcaaa aaaatacttc
    22381 gcccgaaata cgcatagaat catggaatgc acttgaaagc aaaaccctgt cctctgattt
    22441 cttttccagt ttttatttcg gtgaatacat tgattcatcc tcaaaatgga atgaaattga
    22501 acatctgaaa gaaaaaaatc acattggatt tttcagcaat caccggattg aagtcatgtt
    22561 gccatctgaa aaaaaacatt cactgtattt ttccggatcg caccaatcgt tgctgggagc
    22621 cggattcagc aaagatctgt ttcagcttgt tttcaccgga aacaattcac tgatcgggca
    22681 gcatgtaatt accaatccgg caacaatgaa tcaatacagc ttctcgacct tatcggcagg
    22741 gattgcatgg caaataaacg atgatatgaa aattcatgct gctgccggcc ccgttgtctt
    22801 atactccttt tcgcgccttg atttttcgga atcagacttt ttcacttctc tgagtgcaga
    22861 cagtctgtcg ctgaaccttg acggccatta catgcgcgcc ggcggaaaca cattcatcaa
    22921 agggagcgga ttcggtgccg aactgggtat tgaaggcaca aacaataatt tcgaatggcg
    22981 tgttacactt tccaacatcg gacagatatg gatgaacaag cgcaccattg attcgcagca
    23041 tgatacactg ctttttttta cgggagtcga aatttctgat ttatccaatt tcagtgcagt
    23101 tattgatgaa gagcttgata atttcgaaga gagctttagt ctgaaaggcg acactgccaa
    23161 cacttcaatc tcgcttccaa tgctgattac aggcgaatgc cgcgcagagt tcggcaaact
    23221 cagaacagac ctgaatattt tatattataa tctccccggc tttatcccat ttgtgcaaat
    23281 acgtccttca tggccactta cagaaggaat acgaatttcg ctaccaataa aatacggtgg
    23341 ctatggctca ttcaatgcgg gacccggact ggaatttaaa atttttgaaa agctttctgc
    23401 acaactggat ttccctgctg cactgtcagt gctgaatata aacagaaatc tgtcctacgt
    23461 tgttgttgga agaattactt ataaaatcac tgaacatgcg agtcttttat agcatattgc
    23521 tgttctttgc tgtggcattg ggttttgccc aagatgcatt tatacggaca tacggtctcg
    23581 atggctcatt caatgaggga aaaggcattg ccgcattttc cgattcaact tatatcatgc
    23641 ttggcaaccg acaaactttt ggtggacaat cggcagcatg gctctttcgg gttgattcaa
    23701 acggcacaat tctctgggaa aaatattttg attcctatgt gatttcaatg gcggaaaatc
    23761 tttcgaaaca cgacgacact tccgttataa ttacaggaac agtccttgca ggaaacgatt
    23821 attcaatgct tgttgcacgc gttggtttat ccggatcggt catctgggaa aagattattg
    23881 gaacccagtc atgggatcag gcactttgct ctgcttcgga tgccgatgga aacatttatt
    23941 tatctggtta cggacaagcg attgacacaa tggatcagga tattcttctg tataaacttg
    24001 acggaaatac aggcgactcc ttgttttcaa aaagacttga tgcaggatat aatgacaagg
    24061 gcgtttatct cgataccatg tcgggaaaca acctgctact tatttcgcac agttataaac
    24121 cattttccga ttcagttatg agtcgcgtat ggaaaatgaa tgtcgatttt gataccctgt
    24181 ggacttggat gccatgtgac tcagctgaat ttgaaatcaa ttgcattttt gaggatactt
    24241 ttcacaggat tgttttttcc ggaaaaatgt taccggatac cggtcaggta tttcgcttct
    24301 ggtatggatt tctgaacgat tcaggcattt ttggctggca gttgattcac ccgccctatt
    24361 ttctgaaaaa catcagacgt ggaattctcg acagcaacaa tgtttcgtat tataccggcg
    24421 gatttttccc tgcctatttt ggctttggca acagcgatat cggcttctgg cgcgacagtc
    24481 tgggatgggg attttcgcac aactacggtg cactgcagga cgaagaaggc atggacctcg
    24541 attttgctgc cgatggagga atggttttta tcggaacaac taaaaattat ggcgaaggcg
    24601 tgaataatat tttctttgtc aaagtcggac ccgattattt gtacaatgat tcggactatg
    24661 cacactatac gcctgtttca ccatctgaaa atatgccagc cgcaatttat cccaacccaa
    24721 gcagcggcac cttctgcatt accctgccaa acattcagga atgcaaaatt gacatataca
    24781 atctacaagg agtgctgata aaaacagaca gatacaattc cggtgaaaca tacacactga
    24841 aaggatttga gaatggagtt tacctcataa aaataacttc cgatcatcag tcttataatt
    24901 tcagattact tttgcagaat gattaacatg aatatgaaac agaaaaaaat actggttttt
    24961 gctgctcctg tttttctggt tgcactggct gtaatactta tcaatccttt cggacaacag
    25021 tctgcagtat tcggaaaaat ttcaccggta gcagaaagcg actccgtcac attctgggtt
    25081 accggcgatg ccatgatgca tatgccacaa tacaaagccg catacaacga aaacaccgga
    25141 ttatgcgagt ttgattcatg ctacacctat ctacgcccga tactacaaaa cgccgatgta
    25201 cgactgatta atttcgaaac aacactggct ggcgaaccat attcaggtta tccaaaattc
    25261 agcgcacctg tcgaatatgc tgatgcctta cgcagagcgg gattcaattt ttttgtgttt
    25321 gctaacaacc acagttgtga tcgcggagct aaaggaatcg aagggaacat tgattatgcc
    25381 gacagcttga aactatggca caccggcatt ttccgcaatc ctactgaacg actggcccgc
    25441 tttccgtata tgctcgaagt gaaaggttgg aaacttgcga ttctaaacta tacctatggc
    25501 acaaacggaa ttcctgagcc agagccctac gtagtgaacc gcatcgattc ggcacaaatt
    25561 ctttctgaca tcaaaaaagc aaaatcacaa agcgccgatg ccatcctggt ttccatacac
    25621 tggggcgaag aatatcaaca taagcaaaat gcaaatcaaa aagcgcttgc gaattttctg
    25681 cttgaaagtg gcgtagatgt catcatcggc agtcatccgc atgtggtgca gcccatggaa
    25741 ttggtgacgc ccatcggaag catgaaggaa cggctggtga tttattcgct cggcaatttc
    25801 atttcgaatc agaaagaccc gctcaccgat gccggactgc tggtaggatt cacgctgaaa
    25861 cgcacgccat acggcaaacc cgaagtgtgc aatgtgaagt tcattccgct ctaccgcatg
    25921 aaactcgaag gcaaaaaacc cggctactac atgatgcccg gactgatgac cgaaaagaat
    25981 atcgacgcgc tcattcccgg cgatgaaaac aaggaagact tccgctcagt gatggaagac
    26041 acccgccgca aagtgaatac cgatatacgg atcagagagg tggagaagta atttcaaagt
    26101 tgaattatgg acaactaccg tttcggcttc aatggcaaag agattcagag tgagtgcgaa
    26161 aaacatcagc ttaaagctta aaaatttaag gcttgaaaat ttgttcaatc tttttgcatc
    26221 aacatcaggc cttatgtttc ccgtcatttt accgtcagtc tgaagggcct ttcaataaag
    26281 cattgctgac gtggaatcgt cgggggaaaa taatgaaagc acccaacgag tccgagtccg
    26341 caaaaaagca tatgagtgaa acatggtcac gcgtcgggag acgcgcgacc aatgttgaga
    26401 atgtttctgg cggccgccaa aatgttgggg cgtacgattc aaatatttgg gaaacgcact
    26461 cctgagaagg aacacacaga caaattattc gacggcaaag gttaaaaaga atattgcaaa
    26521 actttaaaat gacggacagg agattgcacg ggaacgcttt ttatcaaccc gctgattctt
    26581 cggatatttg attgaataat tcagaatcaa aagttttgtt tcgcttggct gcaacgtcat
    26641 tttccatgta atgataccgc tgagcgggtc aagtgttcca ccggaaatat tgatgacgtc
    26701 aacagtgata tcactgttct gcgaaacagg cacctggtcc tggagcgtaa tgttcaccgc
    26761 gcattcctta ttgttgcgta caataatttc atacataaaa gtttctttga tggagctgcc
    26821 gataacctga ctttttgaat cctccatttt ctgtgcacgc ttgataataa tacgtttgtc
    26881 gcgacccagc gaaatactca tggtatcggc aacggttgac gtgttgatgt aactttgtcc
    26941 gacaaaagta ccagcgtaaa aaactgatgc atggccgctc acaagattga gtttgttcca
    27001 gtcggtgatg cgtgccacca gaaatgcatc acggtccatt tttggaatcg ctgaatattc
    27061 aaacactgcc ggcagatcaa acgtgtttac atcgactgtg taagcttttg aatcggaaag
    27121 gatcgtgtat gggacagcaa tttcaaattc ctgactaagt tcgtccacac ggattgtttc
    27181 ataaccggca atggcagctc cttctacttt tgtggcttcg attgtcatac gttgatcgta
    27241 attcatcaca ttgctttcag aagcatcctg cacatcgtag ccataattca gcgcccactt
    27301 atcaagaatg ggtttggtag cgcttttacc gggttcggcc gttgataatt ttattttcac
    27361 atcattccag tcaacaccag aattgttgaa taaattggct ctgtaaacca tttgaatcgg
    27421 cttatcaaca ccatcggcgc ggatatcata tttaggggcc cagccgcaac cactcaccat
    27481 ataggaaaaa tcaaacttta ttttttcttc gcgctgactc agcacgctaa gctcaatttc
    27541 ggacgaaggc ggattgtaag ttgcatttag ttcatccaat tctgccatca ggcgagccaa
    27601 agtttcgcga tattttgatt cattttttgc aagaacaaat agttcatcgt tgatttgatt
    27661 gcttcgcaag cggaaaaaat cggccgagcg ctgaatttct tctactttca ctccattttc
    27721 agtgccgcca atactctggt ttttaaaaag aagatccttt tccttcacaa atgtctcacg
    27781 gctcatagca atgcgtgtca gccgatcatt gataagttca actgagtctt tcagcaatct
    27841 gatcttttta ttatctcctt gaccgttcaa ataattaatt ctcgaagaca cacttaaaat
    27901 agttgcatca ctgttctcaa cactgaaacg aatactgccg gcattcaact gagggctcaa
    27961 acccgggagc agaaccgtgc atttcccttt aggcaaaaca gcttcggccg agcaatgcag
    28021 ctcagcaccc agcatatata cggtaacagc atacacctgt ggttttgtcg ttatggtttg
    28081 agctcctgca tgattgatga taaaaacagc aatcaacaac ataagaattc ggaatgcttt
    28141 catataattt gggtttttac aaacttacaa aaaacaaata gcattcatgg aaatttttaa
    28201 ttttgcaaaa aaattattat gaaaccaatt attccgttat tatttgcaat tcttccgctt
    28261 attgcaactg cacaaaatct atctgtcagt ccgtttttca caagcagtaa ggggttgctt
    28321 tcaaccacct ggcacagcac aaacgatcac acatttgtaa ccatcgacac ttcaatcacc
    28381 aaatactcgg gcgaatccac aaacatttca ctgggagccg ggaacaggta tggtgtaaac
    28441 atagaaaaaa aataccgcga tctgatttca ttcggtttat cactttccta tttcagcagc
    28501 aaggatgtac cgttattatg tacatcaaaa gcggaatacg acccctattc cgcatacaat
    28561 ctgtcattca caacaaccga tatttacaaa aacaaatcgt ttgatctcgg catctattca
    28621 aacttcatca tttacaataa aagtacagca ccatatgtaa aaacaggcct tattttcagc
    28681 ttttcaaaat acacacttga ccgtgaggtt tatatcttca acaacattcc cggatactac
    28741 cctacagaaa gatataatta caattataaa atttctcctt cttttcatta tggatttaca
    28801 ggtgcagccg gcattgaaac aggcagaaat aaaaccattt cgctgtatgc agaaatctat
    28861 tcagttttta tgaacgtttc tcccacaaag caaatatgca cagccaaaac atggaattcg
    28921 gaggatcaat ttgaaacaat gacaacaagt gacaaggaaa ctgtttttgt tgaatcatac
    28981 tcggaagatg acaataaaaa cgaagatgaa ccaacaaaaa acctgaaata taatcagtca
    29041 ttctgtagta ttggcttgca ggcaggtgtg aaaattcatt tctgattgtc acctccgata
    29101 ttgcaatagc ttttgcaacc taattgctgt ttttaaattc ttttttgccc gactgccaat
    29161 tgcagttaaa caggcagaca ggttcagcgt tcttgttctt ctttcaatga ttattcgtaa
    29221 ttttgaggca taaacctctg ctttcgatgc cacacgatat agaaaaaaat ctttcacagc
    29281 tcaaaacaca tctttcaggc gagttgcatt tcgacaaagc ccatcgcatc atgtatgcca
    29341 ccgatgcttc ggcctatcgc gaatttccgg ttgccgttgc ctatcccaaa gacaaagaag
    29401 acctgaaaac actgattcgt tttgcacggg aaaacgacat aacaataatc ccacgtgctg
    29461 ccggaacatc gcttgcaggg caggttgtag gcaatggtct ggttgttgac atgtcgaagc
    29521 attttacaaa aattcttgaa ataaacgaaa agaagcgctg ggcaatcgta gagccaggag
    29581 ttatcctcga tgaactcaac cgggagttgg ccaaatgcgg attgtttttt ggtcctgaaa
    29641 ccagcacatc atcgcgatgc aatatcggcg gaatggtagg caataactcc tgcggcgctc
    29701 atagtcttgt atatggctcc acacgcgacc atctgctttc tgtaaaaacg ctgctcagtg
    29761 atggttctga agccgaattc aaacaattat gccgcgatgc tttccgcgaa aaaatgacgc
    29821 ttgaaaatct ggaaggcaat atataccgta acatcagaga aattctttcc gacgaagaaa
    29881 accaatacaa catagttcat gaatttcccg atccggcaat aaaaagacgt aatactggct
    29941 atgccattga cctgctgatg tatgcagaac cgtttaccgg aggcgagcca ttcaatttct
    30001 gcaaactcat tgcaggatca gaaggcacac tggcactgat gactgaaata aaactcaatc
    30061 tcgttccatt gccacccaaa gaaaaagcgt tggtttgtgt tcactgcaaa tcgctcgaag
    30121 attcactcaa agccaatctg gttgcactca gatatcatcc tgtttcggtt gagttaatgg
    30181 ataaagccat tcttgatttg acaaaagaaa atatcaatca gaataaaaac cgattctttg
    30241 tgtatggtga gcccgctgcc attctgattg ttgagtttgc cgaagaaaca caggaagaaa
    30301 tacaacaaaa aatagcagca ttggaagccg ctatcagagc ggaaaagctg ggatatcatt
    30361 ttcctgtaat caccggcagc gatataaaaa aagtctggga tctccgcaaa gccggactgg
    30421 gagtcctgag caatatgcct ggagattcca agcccgttcc tgtcatcgaa gatactgcag
    30481 tcaatcctga agtactccct caatacattg atgaattcaa cgctttgctg cgcaaacata
    30541 atctcagctg cgtgtattac gcacatattg gcaccgggga actgcatctg aggccggttt
    30601 taaatcttcg tgatccaaaa gatgtaatgc tttttcacac catagccctt gaaacggcaa
    30661 aactggtcaa aaaatacaaa ggttcactca gcggcgaaca tggcgacgga cgactgcgcg
    30721 gtgaatttat tccgctcatg atcggcgaaa aaaactacac gctgctaaca aaaataaaac
    30781 aaacatggga tccttttggg gtttttaata aaggcaaaat cacagacacc cctcccatga
    30841 ataccatgct gcgtttcgag ccaggcgcac aagtcaggga aatagaaacc tattatgatt
    30901 tcagccacga tcatggcatt ttaagagcaa ttgagaaatg caacggatcg ggcgattgcc
    30961 gcaaaccttt tcaggcatca ggcggcatgt gccccagtta tcaggccaca ttggatgaac
    31021 agaactcaac ccgtgccaga gccaatattc tgcgagaata cctcacaaac agtccgaaaa
    31081 caaatccatt caatcatcgc gatatctaca aagcaatgga cacatgcctg ggttgcaaat
    31141 tatgcaaatc tgaatgtcca tcttcggtag atatggcaaa atacaaaact gaatttcttc
    31201 aacattggta caattcgcac ccagtgcgga tccgcacact tgccattgcc tatatcaaca
    31261 gcataaaccg gctggcatta ccgttcagcg gcatatacaa cctgtttgca cgcaactatt
    31321 ttttatcctg gccaattaaa aaaattattg gttttgctcc acagcgcagt ttacctgaac
    31381 ttcatggaaa atcgctccgt cgctgggctg tgaaaaatct gccggaactc aataaactta
    31441 caggacataa tggaacagta catgtttttg ttgatgaatt tactgacacc aatgatattt
    31501 acatcggtgt aaaagccatc agtctgcttt gtatgctcgg ataccgcgtt gaattacccg
    31561 aacacggact gagtgcacgc acattcattt cgaaaggatt gctgaaaaaa gcaagaaaaa
    31621 ttattgacaa aaatatcgaa gctatttcgg gcagaatatc cgaagaacag cccctgatcg
    31681 gcatagaacc ctccgccatt ctgggattca gagatgaata tcctgaatta tgcagtacgc
    31741 gtttgcagga aaaagcaaaa aatattgctc catataccat gctgattgat gaatttattg
    31801 tccgtgaatt tgaaaaagga aaaatcagta aaatccagtt tactacggaa aaaaaagaca
    31861 tcctttttca cggacattgc cagcaaaaag cattgcttgg gacagaagtc acaaaaaaaa
    31921 tgctgagcat cccatcgcat tattccgtga aggaaatacc ttcgggatgc tgtggtatgg
    31981 ccggttcctt cggtttcgaa aaagagcatt atgcactcag catgaaagtc ggtgaattgg
    32041 ttttattgcc ttcagtccgc aatgcgccag aaaaaacaat cattgctgca tcgggcacca
    32101 gctgcaggca tcagataaaa gacggtaccg gaaaaatcgc cttacatccg gttgaaattc
    32161 tgtttgaagc tgtaaagtcg tagcgcagtg tttatcgcat gattcgtgtc tccgatttcg
    32221 tattttccaa actccttctc agatcaccga cgcttcggtc ggcatattct actgatatct
    32281 gattactgac aactaaaagc tatttgtaac gatcgtaacg actcacgcct tcagcgtgat
    32341 aaattccacg tgtaacgaaa cctatttcaa aagctcttta atcagttcaa tgctgcggtt
    32401 ggtggggtcg gtttttttca acgtaaactc gaccccttcc actctcactt tatgataacc
    32461 cgggccaaag cactctttgc gcgtcgtgtc aataaaaaac atatcgtgca cttcaccgcc
    32521 ctgccagggt cgtttctgtt gaagcgccag ttcaagcgtc ggctcattaa aaccatccgg
    32581 cgaaagaaaa atcgaaacaa tatttgggac aacaaaacgc atagcttttg gcattttgta
    32641 ctgagcattc acatgttgac gcgcagcttc atgcatgaca gcacattttt cagaggtaaa
    32701 attcacaaaa ttaccttcat gaaaaaaata ataatcgcgt cccagcatat taaagcgccg
    32761 ataaccatac aaatcggctc tgaatgttcc gaaagaataa ttattctgca aagcaaaagc
    32821 ctttttctga gactcgagaa agcgtgaaaa cgcagtattg tccatgatat cagaaattaa
    32881 taataatacg gcaaagataa ataaactctg ttattttcaa agaaaagaca aacattttat
    32941 gtacttttgg gcaatcaatc aacagcaatt ttcatcagga atatgaaaga cacccggtat
    33001 atttttgtta caggaggcgt agtatcctca ttgggaaaag gaataataag cgcttcactg
    33061 gctcggctgc tgcaggcccg cggatattcg gtcaccatac aaaaactcga cccctacatc
    33121 aacgtcgatc cgggcacact taatccctat gaacatgggg aatgctttgt aacggacgat
    33181 ggagccgaaa ccgatcttga tctgggtcat tatgaaagat ttttaaacat cagaacatcc
    33241 caagccaaca atgtaaccac cggccgcata taccaaagtg ttattgaaaa agagcgccgc
    33301 ggtgattatc tgggcgagac cgtgcaagtc attccacaca tcaccgatga aataaaatat
    33361 cgtatgcgct tactggcatc gcacggcaaa tacgatttta tcatcactga aatcggcggc
    33421 accgttggtg atattgaatc gctgccctac atagaagcag tccgacaact cagatgggaa
    33481 cttggtaatc atgcagccgt cattcacctc acactggttc cctatcttgc tgccgcaggt
    33541 gaattaaaaa caaaacccac tcagcattct gttaaaacat tgcttgaact gggagtgcaa
    33601 cctgatgtta ttgtgtgccg ttcagaaaaa cacatcaatg aatcattgcg aaataaaatt
    33661 gcacttttct gcaatgttca gcccgatgca gttgttgaaa gtgttgatgt tgaaaccatc
    33721 tacgaagtgc cgctcagaat gcgcgatcag cagctcgaca ctgttgtcat gcgtaaactt
    33781 ggcatcaatg aaagcccgga atcgaatctg caggtatggg aagattttct gtttcgactg
    33841 aagaaccctg tagtagaagt caatatcgca ctggttggca aatatgttga gttaaaagac
    33901 gcatacaaat ccattcttga atcattcatt catgcaggtg cagccaaccg ctgcaaagtg
    33961 aaagtaaaac tggttcacag tgagtttgta aatgaagaaa atattgcaga tcaactggct
    34021 ggcatgtcag gaattcttgt tgctcccggc tttggagagc gtggcatcga gggtaaaata
    34081 atatccgtac ggtacgcccg ggaaaacaac attcctttcc tcggtatttg cctgggaatg
    34141 caggtagccg ttatagaatt tgcacgcaac gtgctgcatc tcgaaggcgc gcacagccgc
    34201 gaaatggatc gccggacacc acatccggtc attgacatca tggagtcgca gaaaaaagta
    34261 tcgggtaaag gcggcaccat gcgtcttggt ctttatccat gcaaaataaa agcgggaaca
    34321 aaagtatctg aagtctataa cgaagaactc atcaacgaaa ggcatcgtca tcgctatgaa
    34381 ttcaacaaca aatacattga aatgtattcc atggcaggca tgcaggcagc aggcattaac
    34441 cccaatgaag agctggtcga aattgtggaa atagaaaatc atccatggtt tgttggagtt
    34501 cagttccatc cggaataccg cagcacagta gagaaccctc atcctttgtt cgttcgtttt
    34561 gtcaaagcag ccatcgatta ttcagaaggc agataaacgg atcaaaccaa aacatttgcc
    34621 aataatcatt acctttgcac ctcaatttac gcgcttatta tgaaatataa tcagattctc
    34681 gggtttgttc ttatttttgg aatccttatc ggtttctcga tatggaatgc accttctaaa
    34741 gaagaacgcc agaaagaaca agcaagaaaa gattccatcg ccaatgttca gcagctacaa
    34801 gactcactca atgctgttgc tatagcacag caaaaagatt ctgctaaaac tgtaaccgac
    34861 agtgccgcaa aacaaaaccc aatcgtgagt gcagcagcaa ctcaggacag tgttataacc
    34921 attgattcaa catcaaaatt cacatacgaa aaagatggtt ttttctacac atcaatggat
    34981 ggaaaagata caagctatat tgttgaaaca ccactgatgc gcatttccat tgcatcaaaa
    35041 ggggcctccg tcaaacagat tgaactgaaa aactttttga cctgggacaa aaaacccctt
    35101 gaaatgtttt acaaaaacaa taattatttt gatctttcat ttcatacaac aaaatcactg
    35161 gcaaagactt cttctctgta ttttgaaccc atacagggat ttgacgataa caaatacaca
    35221 acgcaaatta acagcgattc attacttatt tcattccgtg ctcccgccat gaacaacgac
    35281 ggcactgaag ccggcgccct gatattcaat tacacttttt acaaagattc atatctgttc
    35341 cgatttggaa ttgctttcga aaaccgcagc aaatttattg gttccggtat tcaatttatg
    35401 gaagttgact ggaatgcaga tcttggaaca aaagaaaaaa gtcttgacaa tgaaagaaat
    35461 gtcagcacag tccattatgc cgacaacgac cttgaaaccg attatctgag cgaaacaaaa
    35521 gatgataacg aaagcatcaa aacatcgctg aaatggattt ctttcaaaca acaatttttc
    35581 gccacctctt tcattgctga cgagaaattc aattctgccg atattaagtc tttcacacgt
    35641 gaactcagcg acagcacata cctgaaaaca atgcattcgg atatgaattt cgaactcaat
    35701 gttgacaagg acagtgccgt cggattccag ttttatgccg gaccgctcaa atacaaaaca
    35761 ctgcgacaat acgaccttgc acttgaacga caaattccgc tcggatggag ttttgcaccc
    35821 ttggcatgga ttaaccgtta tgctgttatc cctgttttca actggcttga aagttatgca
    35881 ttgaattacg gaatcattat tcttattctc accatattac taaagattgt tctgtttccg
    35941 attgcgctca aaacctataa aagctcggcc aagatgcgga ttctgaaacc ggaagttgat
    36001 gccattgcag ccaaatttcc gaagaaagaa gatgccatga aaaaacagca ggccaccatg
    36061 gatctgtaca agaaagcagg cgccagtcct ctttcgggat gcctgcccat gttgctgcaa
    36121 ttcccgattc tgattgccat gttccgcttt ttcccggcat ccattgaact acgtcaacaa
    36181 ccattcctct gggccgacga tctttcttcg tacgactcca ttctggatct gggcttcaat
    36241 attccgttct atggcgatca tgtgagcttg ttcacattac tgatgaccgt ctcaactgtt
    36301 atttatactc gactgaacga gaaaatgatg ggcagcacca atacaatgcc cggcatgcgt
    36361 actatgatgt atctgatgcc cattcttttc cttggtttct tcaacagtta ttcttctggt
    36421 ctgagttatt actatttact ggccaacctc atcacatttg cacagatttt tgccatccga
    36481 tattttgtga aagaagacaa agtgcgtgca ttgatcgaag ccagcaagaa aaaaccagta
    36541 aaaaaatcaa gctgggcaaa acgcctcgaa gaagcgcaga aaatgagcgc cgccagacaa
    36601 acaaagaaga aatagaattc agaaatcaga atgttgattt gtgattttag aagtgatgag
    36661 tgaattgtga agctggattt agaataaaat taaaatggcc ggaaaatacc ggccattttt
    36721 atattccttt ttgcgtacaa aacaaatatt ccattcacgc cacgtcataa taaactcttg
    36781 gagtgaaacg aagaatatag aatcgtcggt tcgaagcaaa cataacgatt ccatatcctc
    36841 tgcgacttcg tcgctatcgg acgatagaat attatgtttg ctcaaatctg caatcttata
    36901 tcggtcactt tttccccatc tggctggcaa cagcaagaat gacgcccaga accactccca
    36961 gcaaggctgc aaagagcacc tggaattcgc cgggaagaat gaatgtgatc gtatgtgctg
    37021 gaatccagaa aaacggaatg gtttttttga aaacaaaacc ccactgcacc ttccagttga
    37081 tatcggtcat gatttgtccc atcgggatac attttaccag gcaggaaagt tttccaccat
    37141 tgtgctctat gtgtgagtcg gtaattttat gaaaagtcat catcatagga gcgaaaatga
    37201 tgttcagaaa ggcgctaaca aaaaaagagg tgaataattt tgtaattgtg aaatctcctt
    37261 tcagtgcagc cacagcgcct tcgagtccaa atacattttc cagcatagct gaaacgccgg
    37321 ttttaaaaac gaaaaatgca aaaaagataa acataccaag aaagccccat accacagctc
    37381 ttggaagaat tccaaatccc ttataataat aatttcccgt ctttattctt aaaccaagac
    37441 actctccaag agtagccaga acagcaaatt tcaaaacact catcaaaaca ccgtgctcat
    37501 tggtcatggt tttaaaccat tcaaatgcat caggaaaaat aaaaaaagga acaaaaatga
    37561 ccagaaaaat tccggcaaaa acgaggtctt taattttcat gttatttatt ttttctttct
    37621 tactcccgta aaactaccgc ccaaagttaa agcaattata tttgactgaa caatatgact
    37681 atatggtttg atatcaatac cattcacatt atccgtaaat ccaccgttca taaaattctc
    37741 gaaataatac tgaactttaa gactcatttg atttggaaat gttattccga caaatgccga
    37801 tggctggaaa aaattggtac gactgctgaa ccattcctta aaaacaaggt cttttttatc
    37861 gttgtaaaaa atcttttcct tgaagtgaaa aggaacagaa acagcagcac cggcaaaaaa
    37921 gaatgttgga ttattcttat caaaattacc aattttgaaa ccgatgggca tcgttgccaa
    37981 atagctcctg tacttgactt tggtatttaa agaatcgtta tactgagcaa tatatccaat
    38041 attctcaacg ccgattccta caaaaaaacc aaatgattca gccacgtcaa aatgaaagta
    38101 ttcactgaaa ttgaaccaaa gtgaaaaacg tgggatctgc gttggattca acgaatcgtc
    38161 aagcataaca cctgtaagtt tcaaatcggt tccggttgta ttataggtat taacctgcga
    38221 ctgcgaaaaa gcaaacgtac acattgccgc acaaaagaaa aaaagttttt tcatatctgt
    38281 gattttataa ttagacattt gtacgatgta aaagccgttt gcgaaacacc atctgtactc
    38341 aaagctaaat caaaatttat ttttttatat tgatgcgggc atcaacagca acaatttttg
    38401 atccatcgcc caaaagcgga ttgatatcca tttctgcaat ttccggagcg gcctgcagca
    38461 tagccgataa acgggtaaga atttcggcaa acctatcctt gtcaactgcc ttttgaccac
    38521 ggacgccttc cagaattttc ttgccgcgca gaccttcaat catcattctg gcttctgttt
    38581 ttcccagcgg tgtcaatcca gacgtaacat ctttcagcac ctcgacaaat attccgccaa
    38641 gaccactaag aatcagatgc ccgaaacctt cttcgcgcga agcgccggca aaaagctcaa
    38701 caccgctcag catgggctga atcagaatgg ccgtagtatc ttttatctgc atcatgcggc
    38761 gaaattcttc caccagcttt tcctttgttt tcacgttcag caccacacca ccgacatcac
    38821 ttttgtgaac agggcccacc accttcatta caagcgggta tccgattttt tctgcagctt
    38881 caacaagcac atcctcgtta tcagaagtgg cttctccggc acgcggaata ccggctgcat
    38941 cgagcatggc agaaacatct tctggaagca gatatccatc tttcgcatga tcaacaatac
    39001 gacgaatggc atcggtatca actgcaggca ttccatcggc ttcgagcgga tccggtgtat
    39061 tcattaccct tgcaagggca cgtccaaaca ccacttcgtc aggaaaaaac acattgcctt
    39121 tttcgacaaa ataatcaact tcgtttttag cattaacaat agaaggcaac accggaaaaa
    39181 ttggtttttt tgccgttttc aggtgtttat caagaacatc atacacatcg aaaacctcga
    39241 acagacctgg acttccgaag ataactgcaa ttgaatcgat atcgttgaat ttattctcgc
    39301 aagtgttcag aatttcatca agttgttgag cagtaccggt agccagaaaa tcaatcgggt
    39361 tagccaccga agaacctgca aataattttg tcaatagttc tttcgatgct tcgttgtcaa
    39421 tatgaggcac attcaaacca ttcgaagaaa gtgcatcggt aagcatcacc gaagggccac
    39481 cggcatgtgt aacaatagcc acatttttac cattgggctt agggaaactg aaaactgaag
    39541 aaacagcagc aagctcttcg cgaccataac aacgcacaat gcctgccttg cggaaaagcg
    39601 catccacagc cacatcgctc gatgccatgg ccccagtgtg cgatgaagcg gcacgacttc
    39661 cggcatcgga tgagcccgct ttaacagcgg caatgcgaca acctttacta attaaggatt
    39721 tcgcatgttt gagtaatttc tgaggtttat tgacgctttc gatataaagc actttcatga
    39781 gtgaactttc tccgggaata aatgaattat cgagatattc aaggacctct tcaacgccaa
    39841 tctgagcact gtttccaaca ctccagacag agccaaatgt tacgcccttc atcatgcagc
    39901 tttccatgat gaacacagcc gtggcaccac taccggaaat aaaatcaatc cctttcggtt
    39961 caagctttgg aatgggactg gtaaagactg atgtatgatt ggaattcatc ataccaacgc
    40021 aattgggacc gatcaggcag gccccggcct cgttgatgat gttcacaacc tgttgttcga
    40081 gttcttttcc ggcagcattt tcttcgctga agccggcact aagaataata aaagctttgg
    40141 tgcctttctg ttttgccaga acttcaaccg tttcaggaca aaagcgggct gcaatggcca
    40201 ggatagccag atcgacctgc ggcagatctt taacaccggc atgacatttc acatcctgaa
    40261 tctccgattc ttttggattc acaacataaa gcgaaccctt aaaatgatta tcaataatat
    40321 ttttgagaat tttgccaccc ggtttttttg tatcctgcga tgcgccaaca actacaatgc
    40381 tctgtggatt gataagttcc ttcgtaatca taagtatttt tttttgctaa agtacttaaa
    40441 tattccggat aaagcagcca taaaatccat gataaaatga atttttaatt atttgtggcg
    40501 aaaaaagtac tatgaataag atagattttg tattttagct gattatttaa aaactatatt
    40561 atgagacgtt tgatgacaat tttattgttc tgcggcatcg gatggagttt gcaagctcaa
    40621 accgacacca tcagaattcc ggttgaagac ccccaaaagg ccgagcgttt atacaacgaa
    40681 gggacggttc tgtatgacaa tgggcagctg acacctgcgc ttaacaaatt taatgaagcc
    40741 atcagtatta aacctgactt tgctaaagct tattataatc gtggcattgt aaaagctgat
    40801 ttgaaagata aaaccggcgc catggccgat ttcaacaaag ctatagaatt gggtccgaca
    40861 gccaatgcct attttcaacg aggcaaaatg aaatacgatg acaatcaacc cgaagaagcc
    40921 aaagctgatt taaacaaggc catagagctc gacgccatgc atgttcagtc ttattattat
    40981 ctcggcagtc tgaaatatga tgaaaaggat tatgagggcg ccattgagat ttacaaaaaa
    41041 gcgcttatag acaaagaaag ttacacctac gcctatgctt acaatgacat gggaagctgc
    41101 tataagatga tggaaaatta tgacgaagcc atccgttgct ataaagaagc caccaaagcc
    41161 aatccaaaac tggcatttgc atacaacaac accggcagtg tttaccgcaa gctggaaaaa
    41221 tatgatgatg ccatttatga atattcaaaa gccatagatg ccgaccctga ttattatgtt
    41281 gcctacaaca accgcggtct ggccaaaatg gacaaaggcg attacgaagg cgccattgcc
    41341 gatttcacca aagccatcga aaaaaatgca gaatatgcct atgcatacaa caaccgtggg
    41401 tcggcccgat acaaagccga gcaatatgct gctgccatcg aagacttcga caaggcaatc
    41461 acactcaacg gtaattattc cacagcttat ctcaaccgtg gcaacgcaaa agaaatgaac
    41521 cgcgacatca acggtgcctg cgacgactgg aaaaaagccc gtgacggcgg catcaagaat
    41581 gctgaatttt atattaaaca aatgtgcaaa caataattaa ttcagaaaaa aacagctatg
    41641 aaaaacatca tcatatccct tgtttgcctc ttggtttcct ttactcttgg agcacaaaat
    41701 gttgaattca acaaagccaa tttttcagac aaaaaagtgc tgaaagaagc cctcaaaaac
    41761 attgaaaccg gcgacaacta tttcagccag ggaatcggag cccgcaaaaa cgcacttgaa
    41821 agctatctga aagcccagaa cgttaatccg aacaatgcaa cgctgaatta caaaattggc
    41881 atttgctacc gtaaaatcaa catcgaaaaa tcgcttgaac atctgcagaa agccgaaacg
    41941 ctgaacccta actgtgatga ctcactgtat tattggcttg gaatcgccta ccagggtaat
    42001 tacaagttca acgaagccat tgaatcttat aaaaccttta aaaacaagtt gtcgccaaag
    42061 caatatagtg aagttgccgg agccctgaac aaaaaaattc aggaatgtga aacggccata
    42121 gaattggttg caaaaccagt caaagttttt atcgacaacc ttggagcttc aatcaattca
    42181 gccaaccgag aatactctcc ttttatcaat gcagatgaat ctgttttaac tttcaccagc
    42241 cgacggccaa atacaactgg cggcaaaatg gatgctcagg atcagatgta ttatgaagat
    42301 atattcattg cagaaagtgc aggcgaagac tggaaagtta taaatccagg caaaccactc
    42361 aacagtaaag tgcatgatgc aactgctggc ctgagtggtg atggccaaac tctattcatt
    42421 tacaaggatg tcagaaatgg agatatttat gaatgcaaat tgaagggtga caaatgggac
    42481 aaaccggttg cactgcccaa aaccatcaat actaaattcc atgaaagctc tgtaagtctt
    42541 agccctgatg gaagaaccct gtatttcatc agcgataaag aaggatccgt tggtggccgc
    42601 gatatctggg tttcaacgct cgacaaaaaa ggaaaatggg gcaaggccat taatctgaaa
    42661 gacctgaaca ccatttacga tgaagaatcc attttcatgc atccggatgg aaaaacgctc
    42721 tacttcagct cgcaaggcca caaaaccatg ggcggatttg atatttttaa atcgacattt
    42781 gaaaacggaa aatggagcga gccggtcaat ctcggatatc cgatcaacac acctgatgat
    42841 gacttgttct ttactattgc agcaagcggc atgcacgcgt acatgatgag tgatacacgc
    42901 aaagacggct atggtgaccg cgacctgtac aaagtaactt ttattctcga aaaaccagtt
    42961 ctgaccaact ccgaagacaa cctgattgca tggcgtaccg agccggtcag cgaaaaagtg
    43021 attgaatcaa cactggctgt aaacaccgct tcgctcactc ttctcaaagg acgtgttctt
    43081 gacgaagcca ccaaacaacc ggttgaagcc agcatcattc tgaccgatct gtcgaaaaac
    43141 gaagaactgg caactttcac ctccaacagc gccaccggta aatatctggt ttcactcccc
    43201 agtggcaaaa actacggtat tgctgtgaaa gctgacggat atctgttcca ttcagaaaac
    43261 ttcgacatcc cgaatgccgc agcatatcag gaaattgaaa aagacatcta cctcaaaaaa
    43321 gttgaaatcg gaaaagaaat tgtacttaaa aacatcttct ttgatttcaa taaatctact
    43381 ctgcgtcccg aatcaaagaa tgaacttgaa aaccttgttc agctgatgaa ggaaaatccg
    43441 acactgaaga ttgaaatttc gggtcacaca gacaatgtgg gttcggcttc atacaacaaa
    43501 acgttatcgc aggcccgcgc caaagctgtg gttgattatc tcatcgccgc cggcatttcg
    43561 tcaagccgca tgaccagcgt tggttacgga ttcgacaaac ccattgcctc gaacgacacc
    43621 gacgaaggac gtcagcttaa ccgccgcacg gaattcaaaa tcaccagcaa ataaaaatct
    43681 gaatattatt tttaaaaccg cccttaaaag gggcggtttt tttatataat ccccatcttc
    43741 ttcatcagaa aagtggcgct cttgttacgg caaacgcccc cgcggagttt gtagtcgaaa
    43801 tgcaattcat tgtttttgat ttcggcttcg aaacacatgt tggataaaac gcccgggtaa
    43861 tcgggttcca tattgcagat ttcaagatca tgcgtggcaa tgatgccata acaatctatt
    43921 gcagccagtt tgcgaatgac ctccatggta ccgttacgct tgtcgtcaga gttggtgcca
    43981 cgcagaattt catcgagcag aataaagacc ggtttgccgg aagccgcatc cataatggct
    44041 ttgagtcgtt tcacttcagc aaagaaatag gattcgtttt cgttaagtga gtcgctttgc
    44101 tgaatactgg tgagcacatc ggccggaaac acccgcgcat gcgatgcaca aaccggcgca
    44161 ccagtgcggg ccagaatcag attcactccc aaagtgcgaa ggaatgtgct tttgccagac
    44221 atattcgaac cggtaagaat gacaaatcgt ttgtctgcaa aatcaacatc attgcaaatc
    44281 cgcttgtcgt gcagtatcag cggatgtccg agatttgaaa aagaaaatcc tggctcagcc
    44341 accatttcag gaaaggcata atccgtatta ttggcagcaa aattggccag actgctcagc
    44401 gcctccgctt caccaatgcg gtcaagccag agcggaagac ttgcagcatg gtcttttttc
    44461 catttcagca aagcatgtaa cgcatgtaca tgatactgca tcaaaccgtt gagaataaac
    44521 aatgcgagaa cattggaaat tgactccagc ccacggaaca aagccgacag tttcgccagc
    44581 tgatgactga tagtcaccga gccattaccg ggaacatttt gcagattgcg cagcatgggt
    44641 gattgaaaag atttttcttc aatccggacg gcaattcggc tgtaattttt aagaataata
    44701 tcaaccttat cggcaccgga aatctcattt ttcaatttct tcatttgcat gccaagcatc
    44761 aacagattaa aaataaaaac caaagaagct gcatacggaa tccaccatgc tccgctgatg
    44821 aatgaagcaa aaatcaacgc tgccaggaca accgggcctg caaaagacag ggttctgacc
    44881 accaaagaca gaacgctggc tgcaccacta ctccactcca ctatgcggcg aaaatcctcc
    44941 gggctgtctt ttgcaagcat gcctgtggcc tgaatcattt gccgaagatc agtttcaggc
    45001 atcaattcct tgatggccag ttgctttgcc tcaatgtcag tattggaatc atcacccagc
    45061 aattttccgg ctaagcgtct attgcccata gtggtcgaag ccctgttcag atgatgataa
    45121 agcgaaaact caccgaataa atccagatcg ccagcattgg gatgcttttc gggtagaaat
    45181 gaagaaccat tgtcaaacgc agatttcccg gacttcaaaa agtccatctc atttctgtta
    45241 atttccttca gggccttggt cagatcaagc ctgtagcgaa tggagtcctg ctttttcata
    45301 agaaacagaa aaacaatcaa cgataatgaa gcaaaaagca gcagcatcaa cgaaaattgg
    45361 ctgatacaaa accaggtagt tatcaggaat gaagcaactg aaagcagtct ggcaaatgcc
    45421 aacatgttaa attctcgatt taacctacga ctttcaaatg tatatttgtc aaataatgtt
    45481 ttataacgat ttaccatgat gaaaaaatat taaaccgggc tggaatactg aagctgcaga
    45541 aaaaaagggt aacagattcc cggcagcaaa acaaaatatc aaaacagcca tgcaaaccta
    45601 cactttttaa atatttcaca cctcagaaac gaataatttc atcaaaaggg caaaaaagtt
    45661 ttggttatcc aaaaagattt tctaattttg cactcccaaa tttgaaaaag gagaattgtc
    45721 atgaagcgta cttttcagcc ttcgaaccgc aaaagaagaa acaaacacgg atttcgccac
    45781 agaatggaaa cagccaatgg ccgcaaggtt cttgcccgcc gtcgcgccaa aggtcgtcac
    45841 agactgactg tttctgacga gaaattacac aagcgctagt ccgtaacaaa atatttcgag
    45901 cacctcgccg tcgaaagcgg ggtttttttg tttattttgg tctttaattt ccaatatgca
    45961 agctttaacc gacattctgt ttgcccgtct tttgctgaaa cacaaaaaaa ttgtgcttca
    46021 gggcattggc actttcaccc tcaccgaaac tccggctact gcacagcgca tggggcgcga
    46081 ttttcagcca ccaaaagcag ctgtaacctt tagcattcag gaagataatg ataaaagact
    46141 gcgcgatata ctgttgcagg acgatgaatt taacatttcg atggaatcag atgccgccga
    46201 aatatgtaac aattatatcg ttggcattaa atttcaattg gaaaaaaacg ggcagtattc
    46261 cttttcaggt ctgggtaatt tacagataac aaagaacggc gaatcggtat ttgttcagga
    46321 tgatgattca tacaattacg gacttcctga attcacagct gacattgtag ccaaacagat
    46381 tgctgcggct ccggtgataa agccaaagcg caaaaggcgt gtccgcgcat ggctcattct
    46441 gctcattctg attattggcg gcggaattac ctcgtggttt ctgattcctg aaatccctga
    46501 aaacgtgctt ccactctggg aaaaagccat ttcggtgttt gacaggaaag aaccggttac
    46561 tgatactaca acgaccaaag ctgttgatac cactcaaaat gtaatggttg aaccagacac
    46621 catcatgcag gatacaaccc aaaaggacac aataataact gaaggacctc tttcatattt
    46681 tgtcattgct ggtagttttg ccgataaaac tgaagcagag actttctgca acaatctgaa
    46741 gcagagagga tatcccgaag ccatggttgt tatttccgaa gccgaccgca gaatccgggt
    46801 tgcttacaaa ggtttttcga cccgtgaaac cgcacttgat tttctgaata aaacaaaaac
    46861 agctgagaac aaaagcgata tctggcttct gcatcagaat ttataggaca tggccaaaaa
    46921 gaaactgcag cactttgccg aaaataaaac ctttgcgcat tatttcgaaa tgccttttgt
    46981 acaactatcg cccaacggac atccgatgcg gggacaatgg aaaaatcagt ttttcggaaa
    47041 cggtaatccg ctcatcattg agattggctg cggaaaaggc gaatatacag ttggacaggc
    47101 aaaacacttt ccaaacaaaa acttcatcgg ggttgacatc aagggagccc gcatctggcg
    47161 cggcgcccga accataaaag acgaaggcat gaaaaacgcc gccttcatac gctcccaggc
    47221 cggcttgctg caactgtggt ttgctcccgg cgaagtggat gaaatctgga ttacttttcc
    47281 cgatccacag ccgggctgtc gtcagaacaa acgcctgacc tcgatgcgct atctcaatat
    47341 gttcaaagct tttctgaacg aaaacggaat cgtacacctg aaaaccgaca gcagggaatt
    47401 gtttgattac acactggaag tgattgccgg aaacaatctt cagctgattg aaagcatcga
    47461 cgatttgtat gccactcccg gcgacgggcc tttgcatacc attcagacct tttatgaaaa
    47521 aatgtggctt gaaatggata aaaaaatcag ctacatggca ttccgcttaa tataatggaa
    47581 aagaaaaatg actttttcga gcaggtctgg caggtagtgg agctgattcc ctgtggcaga
    47641 gtaacttcgt atggagccat tgcccgctat ctgggatcgc cacaggcatc acgcacagta
    47701 gggtatgcca tgaacgcatc ccatgccagc accgttcata ttcccgcaca ccgcgtggtg
    47761 aaccgcaacg gactgcttac aggaaagcac tggtttggcg gacccgacgt tatgcagcaa
    47821 cttcttgaaa acgaagacat caaagttatt gatgataaaa tcattgattt cgaaaagcat
    47881 ttctgggatc cgacaaaaga actgtagaaa atcgaacact gaacctgtct gacggtttaa
    47941 ctgcaacagg cagtcaggca ggcaaggacg gattgattta agaatttgta catccgcaat
    48001 aataccagaa taagcttcag agactgtttg aaatttattt ctttgacaaa agcaacacaa
    48061 ccttcaatag tctgaaatgc aatggaggac atgagagagt cagatattta taccaaaatg
    48121 aaatatataa cagtctgatt tcaatggatg actccggagg agtc
//
LOCUS       DAOPKE010000012        47280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_797_length_47280_cov_12.359238, whole genome shotgun sequence.
ACCESSION   DAOPKE010000012 DAOPKE010000000
VERSION     DAOPKE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_47280_cov_12.359238"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKY63_02955"
     CDS             <1..214
                     /locus_tag="PKY63_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99604.1"
                     /translation="TGWDTDSYSMRVYSRWGELVFSTTDVNHEWDGMMPDGVTEAQQD
                     VYSVMIYVKGLDGSEHEYIRAIALIR"
     gene            530..958
                     /locus_tag="PKY63_02960"
     CDS             530..958
                     /locus_tag="PKY63_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99605.1"
                     /translation="MKIQSVAVLFLLTLSVALDVQCQIAPNRYVMEAFTNEQIELMSQ
                     ERIEYLNYISQHAWEIVDIPEGKLNADYPLLYRVDNESKMALNSALNCGELDSFNILN
                     FKYSIKTERNYYRIEGCSKWLVLKSHKEITAGFNEFKTIN"
     gene            966..5927
                     /locus_tag="PKY63_02965"
     CDS             966..5927
                     /locus_tag="PKY63_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPE99606.1"
                     /translation="MKKYILFAVFLISSSGIIAQNYSMTTGTITTCSGTFYDSGGNGG
                     TYSTNELYTYTICPSTPGAKVSLNFTSFNVEANYDMLTIYDGPNTASPSLGSYDNNVP
                     LSGVVQATTSNASGCLTFVFESDGSIQYAGWQAAISCSQPCQTIQAVFSSSSPAASGS
                     YINVCQGQTVSFSGSATFPQNGTSYTQSLATSTFTWDFGDGTTATGQNVTHTYATEGG
                     YDVDLVVTDNHGCISYNDIDIRVRVSTTPTFSGTNVSPTSICLGQSATLTGSPHMTPW
                     SQPTGATLAGTTYLPDGTGVSYTTQLCFTEFSPGQTVTAASDIVSLCMDLEHSYWGDL
                     TISIACPNGSSMILTDGYSSTATGYVNLGEPVDDDYSSTVGDPYTYCFTPSAATTMDA
                     LVTAGPPTYSYTDNDGTAVSSMSYIPGGNYAAEGAWSSLIGCPLNGCWVITVTDHLGS
                     DDGSIFQWGINFNPALYPSLWGFTPTLATQQWSGTANSGLTTTTSNPATVNPTVAGNY
                     TYTYTITDNYGCSYDTTVTLNVSAGPTITATASPSSVCPGQSSTLTASGGATYNWSTG
                     ATGASVSVTPGSTTTYTVTGTSAAGCIGTATVTVTASPPAAISASASPAAICSGTATT
                     VSASGGSTYVWSPSGSGASFTASPTTTTTYSVTGTTAAGCTGSATVMVTLNPNPTVTA
                     SASPASVCPGQASTLSASGASGYVWSCGSTMVNPLVTTTYTVTGTSAAGCTGTATVTV
                     VVNPNATITASALPTSICSGQSATLTASGGATYVWNTGSTMNPLTVNPTSTTSYTVTG
                     TTALGCSGTAALTITVNPLPAVSASASPANICFGSSSTLTGSGASSYSWSTGQNATSI
                     SVSPVVNTTYTVTGTSSAGCTGTATVSINISASLSISAAASPSSVCLGQSSTISASGA
                     STYTWSPAGTGASFAASPTATTTYSVTGSDAWGCTGTTTVTLTVNPLPTVTASANPTG
                     ICAGQSSVITATGANSYVWSSGSSGASFTATPSGTSTYSVTGTSSAGCTGSATVTLTV
                     NPLPTVTATATPAAICAGQSSTISASGANSYVWNTGASSASISVSPVSTTNYTVTGTS
                     VAGCTGSATVSLTINPLPVVSATASPSMICAGQSSTISASGANSYVWSPGGSGASFSA
                     TPGSTTTYSVTGTSTAGCTGTAVVTLTVNPLPTVSASASPSAICVGQSSTISASGANS
                     YVWSPGGSGTSFTANPITTTIYSVTGTTTAGCTGSSSVSLTVNPLPNIVATASPMAVC
                     AGFSTTVSASGGSSYVWSSGQTVASFNEMPAATTTYNVTGTDAHNCSNTASVTVTLMP
                     GLTVNVTPEVDDICEGSSTTLTASSPGSGVGYIWDTGAGSASITVSPLTTTTYSVTGT
                     DAQGCSGTTTATVNVDPLPVANFSADPLIGCDPTTVHFTDLSTGNISIWDWEFGDGDI
                     SGLQHPSHTYTSGIYNVTLTVTTVAGCQSSLTMSNYVTISPNPVAAFTANPAVVSQDE
                     PTISFINQSTQATLYNWSFGDNAGNDYAENPIYTYSGSGSYVVTLWAENDAGCTDSAS
                     MTVNVKPTYTFYLANAFSPNGDGRNDVLRPFGTGWDTDSYSMRIYSRWGDLVFSTTDI
                     NHGWDGFMPNGAEAIQDVYSVIISIRGMDGKENHYYKGVGLIR"
     gene            complement(5987..6649)
                     /locus_tag="PKY63_02970"
     CDS             complement(5987..6649)
                     /locus_tag="PKY63_02970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPE99607.1"
                     /translation="MNKKILRNTLIFIFILLILDQFLKIYIKTHFLLGEEVNVIGHWF
                     RLNFVENIGMAFGLHLKGAIGKYLLSIFRIIAATAIMWYLLKIIREKKHLLYIFAIAL
                     IFAGAAGNIIDSMFYGMIFESSPVYGSGDNPAVLFGGNYAGFLQGKVVDMFYFPILHG
                     SYPSWIPFVGGDDFLFFRPVFNLADSYISIAVVMIIVFQKRMFPKEPKADNEKRSEVK
                     EG"
     gene            complement(6642..7067)
                     /locus_tag="PKY63_02975"
     CDS             complement(6642..7067)
                     /locus_tag="PKY63_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPE99608.1"
                     /translation="MAKEEKEKSRYSDEELEEFRLLIVAKLDKAKADLALLTEAFTGT
                     NDNDISDTSPTFKVLEEGSQVLSKEENSQLAARQQKFIRDLEAALVRIQNKTYGICRV
                     TGKLISKERLKAVPHATLSIEAKNKMTKKEPRISLPPDE"
     gene            complement(7078..10452)
                     /gene="ileS"
                     /locus_tag="PKY63_02980"
     CDS             complement(7078..10452)
                     /gene="ileS"
                     /locus_tag="PKY63_02980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPE99609.1"
                     /translation="MKGKYNEYKQLRLTELADDVRQWWDSKDIFRKSIETRAGKPEYV
                     FFEGPPSANGMPGIHHVMARAIKDLFCRYQTLKGFQVKRKAGWDTHGLPVEIGVEKKL
                     GITKEDIGKSISVIDYNRECRQEVMKYKDQWDELTRRMGYWVDLENPYITFDNKYIES
                     VWHLLKTLFDKGLLYKGYTIQPYSPAAGTGLSTHELNQPGCYKNVKDNTVVAQFKVVR
                     NERSEFLFNTPGVKGNLYILAWTTTPWTLPSNTGLAVGENIEYNLVQTYNPYTFEPQT
                     VVVGAPLLNRWFPAENAALDIDSYEPGQKNIPFKVLGTFKGKELTEIRFEQLLPYAQP
                     AEGDPFRVVAGDFVTTEDGTGIVHLAPSFGADDFRMGKKYNLGALTMVNKQGKFTEEM
                     GEFAGKFVKPQYHPDYTPEKEKELNVDIELVIKLKKENKAFKAEKYEHSYPHCWRTDK
                     PILYYPLDSWFIKTTDYRDRMIELNNTINWKPESTGTGRFGNWLENLVDWNLSRSRYW
                     GVPLPVWLSEDGSEIKCIGSLEELSKEISKSIEAGFMKENPMKDFVVGDMSKVNYEKF
                     DMHKPSVDPIILVSDSGKPMRREPDLIDVWFDSGSMPYAQWHYPFENKDVFEKSFPAD
                     FIAEGVDQTRGWFFTLHAIATMVFDSVAYKTVVSNGLVLDKNGNKMSKRLGNAVDPFE
                     TIGKYGPDATRWYLITNSQPWDNLKFDSEGVAEVQRKFFGTLYNTYSFFALYANIDKF
                     DFSDTEVPVAERPELDRWILSELNSLIKLVDESYADYEPTRAGRAIQNFTDEFLSNWY
                     VRLSRRRFWKGEMSKDKLSAYQTLYHVLETLSVLISPIAPFFAERLFSDLNSVTGRRN
                     VESVHLCDFPIYNSEYIDRDLEERMQLAQKISSMVLSLRKKTGNRVRQPLQKILIPLT
                     DNKIKGQIESVQKIILAEVNVKELEFFKDDGSFLVKKIKADFKKLGPRFGKQMKELAA
                     AIAALNGAQINQLENEGKISVSLAEGAAEVLVEDVEIITEDIPGWVVMSEGSLSVALD
                     VTLSETLLEEGVAREFVNRIQNLRKERDYEVTDKIRVFVDKTNLAEKAITNNYSYICN
                     EILATELELRDMKGATEAVLIELTDEISVHAIVEKV"
     gene            10648..12057
                     /locus_tag="PKY63_02985"
     CDS             10648..12057
                     /locus_tag="PKY63_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE99610.1"
                     /translation="MSFKSFIFAIVFFISVGGLMVADMIAPHSSHTPDPIKLRDFDEI
                     VASDTLHVIMGYNSVNYFVYKGTPMGYQYEMVQQLCKDMGLFLDLTVNNDVEEAFRCA
                     AFGKCDLIAMELNVAKNRFPRIVFTDSLYKMRSILVQRKDSAGLVQVKDLKHLSGKTV
                     LIPRKTIYQRQLLELEDSLHIDIDIVPLGEVGTEDLCNLVAEGRVDYTICDEHMARTL
                     WAYYPELDFSVIVSKPQPVAWAVNSNSQVWLSKLNSWIETYKKKGNLHFLQRKYFDHP
                     VLYLFGMPHEHSLSGGKISKYDDLIQKYSNEIGWDWRLVASLIYQESRFTTDFQSPSG
                     AFGLMQLMPGTARHMGVNYNSSAEEQLKGGLKLLHYMDTAFAIYIDDESERHKVVIAA
                     YNLGMAHFYDAFELAKKHGKSPITYAVVIECLKCKSKPLYYNDPVVKFGYLNPWYVER
                     FVREIYARYNAYCAVFPEK"
     gene            complement(12357..13229)
                     /locus_tag="PKY63_02990"
     CDS             complement(12357..13229)
                     /locus_tag="PKY63_02990"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPE99611.1"
                     /translation="MAKHRKSSIRLGGSYATLIISISLVLLILGLSGLILLHSKRLSN
                     YVKENIIVTVMFKDSLPEADMLAMQKKMQLDPAVQTCSMVSSEEAAKRMTEETGEEFV
                     EFLGIIPIPPSLDITLKPDFATSEAIDAFVAKVQKESIVGNVYYQKDLVEDINVNSGK
                     VSLILLGFALLVFLISYSLISNTIRLAIYSKRFLIRSMLLVGATRGFIRRPFLFQALW
                     MGLVSSLLAVVLLEGVMMLAYQQIPDLQAIQDDSQVYMLFGAIILSGMFISWISSWSA
                     LNRYIRMNTDYLYV"
     gene            complement(13675..14448)
                     /locus_tag="PKY63_02995"
     CDS             complement(13675..14448)
                     /locus_tag="PKY63_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99612.1"
                     /translation="MKSVIIFLSAIIAFASLPAQETKTHSVYFSTASFEPDAAAMRIL
                     DTVAAYCKKYKASIVSLSGYCDSVGTTERNQVLSERRANAVKSVFVQKGIDVKSTLAA
                     GKNETLEFGDTEFFRNRRVDIVTSRPEEKKMSVLKEKIIQAEAGDLIRLENISFIGGT
                     PIPLPVSRPVLDELYQVMAENPTLEISIEGHICCSRNDEEDLSGQRAKAIYDFLISKG
                     IAAERMTWKGFGHTRPLTEERNASEQQMNRRVEIRVVKK"
     gene            complement(14520..15293)
                     /locus_tag="PKY63_03000"
     CDS             complement(14520..15293)
                     /locus_tag="PKY63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99613.1"
                     /translation="MKHTLFTLLLFILLSPAMAQETKTHSVYFSTASFHPDAAAMRVL
                     DSVAAYCKKHNATVVSLSGYCDSVGTSERNQILSEWRANAVKVIFIQKGIDVKSALVA
                     GKNETLEYDGTDFFRNRRVDIVTSRPEEKKLSILEEKISQAQVDDLIRLENISFYNNS
                     SRPMPESMPVLEELYNIMLSQPTLEIAIEGHICCISDDFSNLSGERAYVVYAYLIKKG
                     IEAKRMSFKGFGHTRPLNEEKTEAEKQENRRVEIRVVKK"
     gene            15796..16488
                     /locus_tag="PKY63_03005"
     CDS             15796..16488
                     /locus_tag="PKY63_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPE99614.1"
                     /translation="MQPIILWQDQYALAVNKPNNVLVHHSDYSGIEGEKSLLELLKEQ
                     GIPRVYPLHRLDRKTSGIVLLAKHRSNVAVFQKLFDEQKIRKTYLALLRGHVDAEGIV
                     DTPVRNDRGNYRDALTHYRCIEHYELNVAVEPYDTSRYSLVEMEPKTGRLHQLRVHAN
                     KIAHPIIGDHKHGNRHHNRLFAEQFLLPDLFLHASELQFTHPFTNEEIIINALLPAFW
                     ARFDEIRKELSI"
     gene            17034..17468
                     /locus_tag="PKY63_03010"
     CDS             17034..17468
                     /locus_tag="PKY63_03010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE99615.1"
                     /translation="MKALINTVKGTMKVEFYENDAPNTVANFVKLAKSGFYDGLTFHR
                     VIPDFVIQGGCPDGTGAGGPGYSIKCETSGGNQYHDRGVLSMAHAGKDTGGSQFFICH
                     SRTNTAHLDRRHTCFGKVVEGLEVIDAIRQGDEIESIKIIEE"
     gene            complement(17518..18399)
                     /locus_tag="PKY63_03015"
     CDS             complement(17518..18399)
                     /locus_tag="PKY63_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPE99616.1"
                     /translation="MSQSKISIRFFDDREVRAVWDEQNARWWFSVLDIIAVLTNQDDY
                     SKNRNYWKYLKAKLKKENSQVVSNTTQLKFLAPDGKKRLADMLDYDGIIALGKEFPGK
                     KANRFIDWFTYSDDSIDGKSKTKAYALFKSSFIDSIEVGTARGLQQIHAYLFGGLYDF
                     AGQIRQKNISKGGFHFAVARFLDETLKQIESMPETTFEEIADKYIEMNIAHPFMEGNG
                     RSTRIWLDLMLKKRLKKCVDWSKISKRDYMNAMMLSPTNSSILKALLKNALTGKINDR
                     DMFLKGIDYSYYYEENN"
     gene            complement(18547..19761)
                     /locus_tag="PKY63_03020"
     CDS             complement(18547..19761)
                     /locus_tag="PKY63_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99617.1"
                     /translation="MQKILRKAELYKLSILKDKNLIKVVTGVRRAGKSTLLLQFQELL
                     KKEHPNVSIISINMDLPEFRFLAEKNWKEIYDHINGMLQKDVMNYVFIDEIQNIPVFE
                     KLLEGLFVTPNTDLYVTGSNAYLLSSELATILTGRAFEINILPFSFAEYLEFTGKESS
                     PDRAFAEYMRIGGFPEAVGLAGSGENFTYEYLKTVFQNIYQNDIQKRHTIYYETSYNE
                     VVHFLIDNVGSTVSARNIARILTANGKKIDNKTVSKYIDTLVESFLFYKISRYDIKGK
                     QHLATQEKYYLVDLGLRPALLGKELAADAGHILENVIFLELLRRNYQVWIGKTNNLEV
                     DFVVRDKDGYTQYIQVAYTVRELQTLERELAPFNRIPDYNERILITMDYETGSHNGVK
                     QINAIDWLLNNQ"
     gene            complement(20121..20294)
                     /locus_tag="PKY63_03025"
     CDS             complement(20121..20294)
                     /locus_tag="PKY63_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99618.1"
                     /translation="MSQSKISIRFFDDREVRAVWDEQNARWWFSVIDVVAVLTRFRRP
                     SPPDGTVGGSPEM"
     gene            complement(20311..21126)
                     /locus_tag="PKY63_03030"
     CDS             complement(20311..21126)
                     /locus_tag="PKY63_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HPE99619.1"
                     /translation="MAKNRKIEIGGTEITIISQKQDDYISLTDMAKSQMQEAIIIKWL
                     SLKSTIEYIGEWEALYNTDFNYTEFGTIKNSAGSNNFVLSVKNWIEATNAIGITAQPG
                     RYGGTYAHKDIAFHFGMWISPKFQLLLVKEYQRLKEDENDRLKLEWNIQRTLAKVNYE
                     IHTDAIKDNLIPPEITKQQASLVYANEADLLNVALFGITAKEWRERNLDKNGNIRDYA
                     TLEQLVVLSNMESINALLIRQGLSQSERLIQLNKVAITQMKSLLASRSMKKLK"
     gene            complement(21392..21892)
                     /locus_tag="PKY63_03035"
     CDS             complement(21392..21892)
                     /locus_tag="PKY63_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99620.1"
                     /translation="MRSIFLIFLLLVSGVFAYGQQRVFNSNNKFSIIPIENWENHSED
                     SSLVFSQSSQGALDLYSENIQIATFPADGKTLDEIWNSWVIEDFPNSFNNYEVMGTGV
                     LIINDKTVRWVDFKNRDFNFNFRDLAFLIVDNDVLYYIICLSLEMDFEKTKNDFFKMI
                     NTLEIY"
     gene            complement(22090..22506)
                     /locus_tag="PKY63_03040"
     CDS             complement(22090..22506)
                     /locus_tag="PKY63_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99621.1"
                     /translation="MKLELKIYLLLSVIALAVILQSCCFGAKEEYLGNNLYLSEFDNV
                     DRQILYQEKSCASSGVEIVPMTVMEIAYNSEWIIAKSENKRDKTNIRYWVIKNKYECI
                     PDAQTVKANTIEFDDYNLFKSFLSDNSILLELEEID"
     gene            23034..25655
                     /locus_tag="PKY63_03045"
     CDS             23034..25655
                     /locus_tag="PKY63_03045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPE99622.1"
                     /translation="MEIPTKYDPTKSEDKWYAYWMNNGFFCSVPDDRKPYTIVIPPPN
                     VTGVLHMGHMLNNTLQDVLVRRARMKGFNACWVPGTDHASIATEAKVVGKLRAEGIDK
                     KSLSRDEFLKHAWVWKEKHGGIILEQLKKLGASCDWDRTAFTMDQNLYDSVIKVFVDL
                     YGKGLIYKGHRMVNWDPSALTAVSDEEVIYKEQNAKLYYLRYYLENSKEYLVIATTRP
                     ETILADTAVCVNPNDERFKHLHGKKVLVPLINRVIPVITDEYVDMEFGTGCLKITPAH
                     DINDYTIGQKFGLEVIDILTPDGKLNEKAQLLIGEDRFAARKKIMPMLEEAGNVEKIE
                     DYVNKVGYSERTDVAIEPRLSEQWFMKMSDIAKPALDAVMNDQVQFHPAKFKNTYRHW
                     MENVRDWCISRQLWWGQRIPAWYLPDGKVVVALNENEALEKAHQINPALKLSDLKQDE
                     DVLDTWFSSWLWPISVFNGIMEPDNKDIKYYYPTNDLITAPEIMFFWVARMVMAGLEY
                     RKEIPFSNVYFTGIVRDKQGRKMSKSLGNSPDPIELMQKFGADGVRVGMMFSSPAGND
                     LLYDDSLCEQGRNFANKIWNAFRLISGWQVDEKLSQPASAALSTEWFGHRLRQTLKQI
                     DEQFVDFRISDALMSAYKLVWDDFCSWYLELIKPAYGQPIDGKTFAETMEYFDQLMRM
                     LHPFMPFLTEEIWQTMKQRGEKESIMIQPMPSDFNHDEKLIAGFEVVQETIMAIRAYR
                     NDKGLGMKELLDVFYTPVQHGETIQKYAPVIMKLGNLSRFEKLSEKPEKSFTVLVRTE
                     ELVFPVSADLDTAAEKEKLMKELDYTKGFLETVRKKLSNEKFVSGAPAQVIDNERKKE
                     QDALSKIAVLEKTLSEL"
     gene            25692..26639
                     /locus_tag="PKY63_03050"
     CDS             25692..26639
                     /locus_tag="PKY63_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPE99623.1"
                     /translation="MLTDVLYIVLALFLLFFGAQWLVTGSAKLAEAYGIPQLIIGLTI
                     VAFGTSSPELIVTITASSQGQTGMAIGNVVGSNIMNILLILGLTAVVSPVFVEKKIIR
                     SDIPLLIVFSSLLIWILINPELKSWEGFILLGCLLLYAGWMFFNIRKKRQLLKADGGG
                     KSATHFSKKQKYFFVFQVFVGLGLLTAGSRLLVLGAVDIARLMNVSETIIGLTVVSIG
                     TGLPELGTSAIAAMRKQSDIAVGNIVGSNIFNILVIAGISISAYPAADSGISLIDLLF
                     MIGAAVLLFPLAYYGSAIRRAEGALLLGLYVLYLFIIWP"
     gene            26759..27556
                     /gene="bamD"
                     /locus_tag="PKY63_03055"
     CDS             26759..27556
                     /gene="bamD"
                     /locus_tag="PKY63_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPE99624.1"
                     /translation="MKFWRNFVLIVLILTGVSCSEYDKLLREGTPQARLEAANEYYSK
                     GNYFKALQLYDQLIVELRGMPQFEEAYYKYCWSNYQQEQYLAAAYYFKNLAKTLPNSK
                     YAEESLYMAAYCQYLESADAALDQTSTNNALTELQLFVNKYPKSEKIPQCNELMDELR
                     YKLEMKDFNTAMLYFKIEEYKAAVVAFTNLLKEYPATKFKEQALFYILKAEYYYASGS
                     IDSKKKERFEHAAKAYTTLVLQFPTTSYKKEADVINEKIKVQISKLN"
     gene            27560..27883
                     /locus_tag="PKY63_03060"
     CDS             27560..27883
                     /locus_tag="PKY63_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPE99625.1"
                     /translation="MDYKKLKIETNAVTRNLHELENETGSIYESIMIIAKRANQISMV
                     IKEDLQHESEQFVSTMDNLEEVFENREQIELAKLYEALPKPTLIALHEFQQKKVYHRR
                     GTQEN"
     gene            27890..29092
                     /gene="coaBC"
                     /locus_tag="PKY63_03065"
     CDS             27890..29092
                     /gene="coaBC"
                     /locus_tag="PKY63_03065"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPE99626.1"
                     /translation="MKNFRVILGVSGGIAAYKAPFIIRLLKQKGASVQVVCTPNALEF
                     VTLPVLETLSENRVYIDMFPKTYQQSAEHISITDNADIMLVAPATGNVIGKFASGIAD
                     DALTTSFMAFDGPVFIAPAMNTKMWNSPVVQKNVAYLKSLGHTIIGPAAGDLACGYKG
                     SGRMLEPHEIVDEVLGSLASSTLGGQRILVTAGPTFERIDPVRYLGNFSTGRMGYAVA
                     SALAARGAEVVLVSGPVHLQADPRVKLIQVESAQQMHNVCVKHFPKCQAAVMTAAVAD
                     FRPKTSAKSKIKKEQNASLNIELELNPDILATIGGMKKAGQIVIGFALETTNELANAQ
                     KKLDNKKADIIVLNSLNDPGSGFGHDTNKITLIRKSGKPQPWPLMSKTEAASVIADEL
                     ENLLLRKK"
     gene            29089..29988
                     /locus_tag="PKY63_03070"
     CDS             29089..29988
                     /locus_tag="PKY63_03070"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPE99627.1"
                     /translation="MRAILILSFLLLFSSVNAQELMCNVQVNSSQVQSSDKTVFENLQ
                     TAVYEFMNNKRWSNYEFRMEEKIECSILLTISSWDNIESFTGTIQVQARRPVYGSSYS
                     TPLFNFLDKDFSFKYISGQPLDYIENTYTSNLTSVLAFYAYLVLGFDFDSFEELGGTP
                     FFEKAQGVVNAAQSAPDKGWRAAESQKNRYWIIENILNNSYADFRRGNFHYHLNGMDL
                     FSSDAVKARAGVTEGVTLVQKAYRQKPGLFIISLFMLAKSDELVSIFTPAPMVEKTKV
                     VNILKSIDPANSSKYNQVLTTNP"
     gene            29991..30200
                     /locus_tag="PKY63_03075"
     CDS             29991..30200
                     /locus_tag="PKY63_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99628.1"
                     /translation="MEAHFDPEFEKELRYYIDLDLASNAICGWGYDLREKLVNVKLKA
                     IQHRIFITKGQYNKLVQKASGIRKK"
     gene            complement(30340..31731)
                     /gene="sufD"
                     /locus_tag="PKY63_03080"
     CDS             complement(30340..31731)
                     /gene="sufD"
                     /locus_tag="PKY63_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPE99629.1"
                     /translation="MTELQKYPVAESLIKAAEKISENDGLWDGRNKAFDAFLSAGFPH
                     KKMEKWRSTDLSEYLNAKYTYIENTDPVSNLNINQVFHCSIENFETALFTLLNGAYVY
                     NTAPLTVMENGVIAGSLRQAWHEFPQLVGQYLSKLTENSVNGLVSLNSALATAGFFIY
                     IPDNIVFDKPVQLVNLVNTEVNPFLQIRNLIVLGKNSKLQFLQCDDSIKDKNSFLNIV
                     SEFHLAEGSHLDHYKLQNKDDSAVMTNTSFFGLQRDAKLSTNTITFNGGNIRNESIVN
                     LNGEGAEAEIMGLYLVDRKQHVDNQVLVRHNVPNCTSHELFKGILDDEASVAFNGHII
                     VKPDAQKTNAFQSNKNILLTDTAQISTKPFLEIYADDVKCSHGATVGQLDDNAMFYLK
                     QRGICERNAKMLLMYAFADEVVRKITVESLYHQTAEMVSRRLKGELSVCDQCMLHCGS
                     KHPIEFEIDVTRI"
     gene            complement(31743..32489)
                     /gene="sufC"
                     /locus_tag="PKY63_03085"
     CDS             complement(31743..32489)
                     /gene="sufC"
                     /locus_tag="PKY63_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPE99630.1"
                     /translation="MLHIKDLHVSINGREILKGVNLDVKPGEVHAIMGPNGTGKSTLA
                     NVIAGREGYEISGEITFEGKNIFDLAVEDRARAGIFLGFQYPVEIPGVSIMNFLKTAI
                     NEKRKFLGQEPMSAGDFLKLVEEKKKVVEIPADLMKRSVNDGFSGGEKKRNEIFQMAM
                     LEPKLAILDETDSGLDIDALRIVATGVTRLKKPENATVVITHYQRLLDFIVPDFVHIL
                     YGGRIVKSGDKTLAMELEKQGYDWIKKEFA"
     gene            complement(32576..32926)
                     /locus_tag="PKY63_03090"
     CDS             complement(32576..32926)
                     /locus_tag="PKY63_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99631.1"
                     /translation="MAGTFNETFRQRTKKFNIDIVNFLEEDRPDVFYGIVKKQLLRCS
                     SSVGANFRALCRSKSERDYKYKLDVVIEEVDESAYWLEILNDTGRFSKKEVEPFLNEA
                     EELVRIFMKVKYNK"
     gene            complement(33011..34459)
                     /gene="sufB"
                     /locus_tag="PKY63_03095"
     CDS             complement(33011..34459)
                     /gene="sufB"
                     /locus_tag="PKY63_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPE99632.1"
                     /translation="MKEDESQNALNEVTGGEYKYGFETHIEMDTIPKGLNEDVVRIIS
                     KKKNEPEFMLEFRLAAFRKWKEMSIPEWAHLNIPPIDFQNISYYAAPKKAEKKSMDEV
                     DPELIDTFNKLGIPLEEQMALAGVAVDAVFDSVSVKTTMQDKLKELGVIFMPISEAVH
                     QHPDLVKKYLGMSVPVTDNYFSALNSAVFSDGSFVYIPKGVRCPVELSTYFRINARET
                     GQFERTLIVADEGSYVSYLEGCTAPMRDENQLHAAVVEIITYDNAEVKYSTVQNWYPG
                     NKEGKGGIYNFVTKRGLCKGVNSKISWTQVETGSAITWKYPSCILMGDNSVAEFYSVA
                     VTNNFQQADTGSKMIHLGKNTKSTIISKGISAGQSQNSYRGLVKVMKKAINSRNFTQC
                     DSLLLGDKCGAHTWPYINVHNKSSIVEHEATTSKISDDQLFYCQQRGLDSESAIGLIV
                     NGYAKEVLNKLPMEFAVEAQKLLAISLEGSVG"
     gene            complement(34665..36095)
                     /gene="pyk"
                     /locus_tag="PKY63_03100"
     CDS             complement(34665..36095)
                     /gene="pyk"
                     /locus_tag="PKY63_03100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPE99633.1"
                     /translation="MSETTLNHTKIIATIGPASSSPEMLRALVNAGMNVCRLNFSHID
                     YAKATEIVEEIKKINADILFPVALLADLQGPKLRVGDMGCNGAELKNDALVIITSEEC
                     EGTSDRFTLRYPMLSRDLKPGEKILLDDGKMEVHITRVLNEKEVEAKVIRAGILKSNK
                     GFNLPGTNVSLPALTEKDLKDLEFALNADVDWVAISFVRKAEDVIELKKRIADSGKTA
                     RVVAKIEKPEAIEQIDEIIEAADAIMIARGDLGIELPLQEVPILQKNIIQKTVTAAKP
                     VIVATQMMESMMTSTMPSRAEVNDVANAVTDGASAVMLSGETSVGQYPVETVGTMFRI
                     IIDVERDSRPYTKGAMPRSEDEVSTPYEISFMASRMAKFMNAKAIVVMTATGSSAVRM
                     SSFRPRSHIFVFSNDRKLLRTLMLAWGLRGFYYDNFQSTDQSICDTIEILKSNNFVCQ
                     GDTIIHTASMPIEQRGRANTIKVSVV"
     gene            complement(36180..37442)
                     /locus_tag="PKY63_03105"
     CDS             complement(36180..37442)
                     /locus_tag="PKY63_03105"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPE99634.1"
                     /translation="MKKLFFVTALMAVVFTSCVNEDFDIPDVIIPEYEGTSNTTIADL
                     KASYPGTLDSIETSTIIEGIVVANDESGNFYKTIVIQDATGGIELKLDRYDMYTSFKV
                     GQKVFVECQGLYLGDYGGLVQIGYIYNDAIGRIPDILVDSHVYRDSLPGNPPAATVVT
                     MPAITPANLSTLIQIDNVHFTEPGLAFSETGGTTNRTLEDANGNQILVRTSNYASFAA
                     DIIPDGTGSIRGILSIFNSDYQLYIRDLNDIVNWYDPNANSVIYDPFNIDPLVNSGWT
                     IFSAAGNKDWYFNSAYKDMAVTASSSDVACDDYLITPAINLSGVANPVLSFSSWTNYT
                     DAGMATPIEVLISTDYSGSGDPTSATWTTLSATWSPTGSATYTPSGDISLTSYSGQTI
                     YIAFHYRSSGNTSGNYSAWKIDDFIIEK"
     gene            complement(37474..40158)
                     /locus_tag="PKY63_03110"
     CDS             complement(37474..40158)
                     /locus_tag="PKY63_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99635.1"
                     /translation="MRILCYLLLLLLPTFMMAQEGTLSGTIKDDITLEGVPDATVKIN
                     NQLIKTDAVGNFSVTLPYGTYTLIVEANLFQMHTEKVSIASPAATISVQLKRKTMEEI
                     GGISEIQLDDSEFDQGGSGQSVNSLLHSGSDIFENFASFSWGSMRFRSRGYESSSDQT
                     YLNGFPMNDLETGFQSYSDYGGLNRVVRYRESYDGLTPGPFSFGGIGGTSNIQATASQ
                     IRKQNQISYGYANRSYNNRLMYTYATGMQNNGWAFAFSGSHRWALDGYVPGTWYDAYA
                     YFGSVERKINDKHMVGLTVFGSPFRRAMQAASTQEAYDLAGTVYYNPNWGMLNGEERN
                     ARVRKVHQPYALLNHTFKINEKTKLYTTAGFTKGRFGTTRLNWYGAPDPRPDYYRYLP
                     SYQPDANINGLVTDYWLNNVEARQINWDEIYQINYSANAAGTQANYIIEEARKDETRK
                     ALNSYLLYKMNENIQISTGLEVNSQTTHYFKELNDLLGGEFWKDIDQFAERDFPGDTG
                     MLQNDLNNPDRIINVGDEFGYNYKLHTKTAKLWGMSRFYYAKTDFYVAGFISGTQMHR
                     DGIYRNGRYPENSFGKSEIKTFIDGGVKAGVTYKYSGKHYFTVNTALISNAPSTMNSF
                     LSPNISNRFVPNLQSSVIFSGDISYIKKGEFVTGRVTAFQTNTFNEVDLMSFYHDEYQ
                     TYVYMNLTGINRVYQGVEFGVDVKASKTISVQAAGNFGNYRYISRPTAHISFDNLSKP
                     DTTELIYCKYFYVPGTPQIASSIGLKYNHPKYWFFNVNFNYFDKIYLDFNPERRTQLA
                     IANLGPGDPMIDEITAQEKLPSGYTLDASIGKSWRIKGKTLAVNFMVSNILNNRELIT
                     GGYEQSRFDFDTQNVGKFPPKYYYAYGRNFLAMVSFNF"
     gene            complement(40298..41080)
                     /gene="ccsA"
                     /locus_tag="PKY63_03115"
     CDS             complement(40298..41080)
                     /gene="ccsA"
                     /locus_tag="PKY63_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPE99636.1"
                     /translation="MTVISFQYIGIVSVGLLLLSIILMLFKRYRNTAVAVASVAVVML
                     TFFIVSLWIELERPPLRTLGETRLWYGFFLSLLGVLLWFRWKYVWLFVYANISAAVFI
                     IIDLLNPAALDQTLMPALQSVWFVPHVVVYIIGYALLGMSALIAAIRLVMPLFGKQFK
                     NSMEYTDNTVYFGFSFLTVGIILGAIWAKVAWGHYWTWDPKETWALITWLTYLLYIHI
                     RLRAPEKQKVVSWILIFAFLFLMITWLGIQYLPAAQNSVHTY"
     gene            complement(41077..>41927)
                     /locus_tag="PKY63_03120"
     CDS             complement(41077..>41927)
                     /locus_tag="PKY63_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPE99637.1"
                     /translation="QLFLLTTLGLTIIRRFSLKPRNAGFMLNHLGLWIVLSGAAFGSG
                     DLERYTMRVEKNNIEWIGSDKNGKQHELPIAIYLTDFTIEYYPPQTGIWSQSQQKLLK
                     NPSFTLQQTDTIFKWESYEFEIKQILKNGWLMGDTFREAPIPGAVQAALVCIRSENAN
                     EKCGWISSASPLQQPFSIAINEDLELLMKPPKARLYQSDIIVYTKEGSEVTSAVAVNR
                     PLTVDGWKIYQFGYDETMGKWSSSSIFELVRDPWLPVIYTGLCLLLCGAIFMFLTGVN
                     SKKKKE"
     assembly_gap    41928..42027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42028..43394)
                     /locus_tag="PKY63_03125"
     CDS             complement(<42028..43394)
                     /locus_tag="PKY63_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPE99638.1"
                     /translation="MKSITEMVSKKPWLGWVLFLGTVLIVFLLGLLAASIIERRAEAE
                     FTYKPDVTFGETEPRSEIWGRNFPREYDSWLKTADTSFKSKHGGSATRDILAEDPRLV
                     ILFAGYSFSQDFKQARGHFHAIDDVHNTLRTGAPKEGVPGPQPSTCWTCKSPDVVRVM
                     KEKGVKEFYTGTWESLGAEIVNPIGCLDCHDPKTMNLTITRPGLIEAFERRGQDVNKS
                     THQELRTLVCAQCHVEYYFDKKKYEGANYLTFPWDSGFTVEQIEKYYDNSEFTDWVHG
                     ISKTPMIKAQHPDYELFMTGVHYKRGVSCADCHMPYKNEGGQKFSDHHIQSPLNNISS
                     SCMVCHREETEDLIDDVYSRQDKIKEIRDQLEIQIVWAHFEAKAAWDKGATEEQMKPV
                     LKLIRQAQWRWDYVAASHGSSFHSPLEVSRIINHGLDKAREARMQLQKILLSLGVKDG
                     VPYPD"
     gene            complement(43398..43985)
                     /gene="nrfH"
                     /locus_tag="PKY63_03130"
     CDS             complement(43398..43985)
                     /gene="nrfH"
                     /locus_tag="PKY63_03130"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPE99639.1"
                     /translation="MIKFVKKLLPPPKWRVPLIFLTGIFLGLSAFLFYVSKAHTYLND
                     DPATCMNCHIMAPQYSTWFHSSHRDRAVCNDCHVPHDNFFNHYYFKAKDGMRHATIFT
                     LRAEPQVIFIKEAGERVVQQNCIRCHGDLLHSGNAKMYTSSYDDFRSERHCWECHREV
                     PHGRVNSLNSVPNARVPKPGSAVPGWLDQMMNKNK"
     gene            44205..45242
                     /locus_tag="PKY63_03135"
     CDS             44205..45242
                     /locus_tag="PKY63_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPE99640.1"
                     /translation="MKTILLLFVLIAFFFGAQAQEKSYNVTCVAFYNLENLFDTINDP
                     NKNDEEFLPEGSNRWTSQKYAEKLSNMATVITQIGDEYIKGGPVCLGVSEVENIVALT
                     DLANEPKMKASGYVPLLIEGPDRRGVDVGFLYRKDFFTMISATSSRLVVADQPDFKTR
                     DQLLVKGILLGDTIHIMVNHWPSRYGGEKRSAPMRAAAAKLTRSTTDSIMNVNPNAKI
                     IIMGDLNDNPTNASVFKILNASGDKKTAMDITGLFNPMYNMFKVQGIGSNAYRDSWSL
                     FDQIIVSKGLLGDDKSSLKMFKTLIFNRNFLKQVEGSFAGYPYRTFVGGQYQGGYSDH
                     FPAYMFLIKEK"
     gene            45516..46271
                     /locus_tag="PKY63_03140"
     CDS             45516..46271
                     /locus_tag="PKY63_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HPE99641.1"
                     /translation="MTALETLLQHRSIRKYKQDPVPEDILNSILEAGSRAATTGNMQV
                     YSIVVTRDQEIKNQLLPLHFGQKMIAEAPVVLTFCADFNRFNQWCNQRNAEPGYDNFL
                     SFMNAAIDALLVAQNCVVAAEHFGLGTVYLGTTTYMADKIIDVLKLPKGVVPITTVTI
                     GYPAEIPGLTDRLPMRAVVHYETYNEFDAASIDALYAEKENSDEYKKFVTDSQVDNLA
                     QVFTQKRYSKKDNVAFSKSLLAVLQKQGFMNND"
     gene            46274..>47280
                     /gene="mnmA"
                     /locus_tag="PKY63_03145"
     CDS             46274..>47280
                     /gene="mnmA"
                     /locus_tag="PKY63_03145"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPE99642.1"
                     /translation="MTSNLSTALLISGGVDSSVAVQMLMEQGIKPDLYYIAIGPDQDF
                     KGFDCKMEEDIDLCKLVARRFGLAFDIVPLHKEYWDMVVQYHIETLKAGRTPNPDLLC
                     NQLIKFGAFEEKVGYKYDRLATGHYAWIQDTDAGRFLHTAKDKHKDQTYFLAQLSAKQ
                     ISRLMFPIGQLTKPEVRKIASELDFPNATRKDSQGICFIGRSHYKDFVEHLLGKKKGD
                     FIEFSTGKKLGEHDGHWYFTVGQRKGLGLAGGPWFVVKKDAETNNVYIACGYEPDEVY
                     GRDIYINQPHFLFPFELNENEEAEVTVKIRHTPTFSKAIIRKTTQGFHIITDELQHGI
                     AP"
BASE COUNT        12696 a        10269 c        10162 g        14053 t
ORIGIN      
        1 caccggttgg gatactgaca gttatagcat gcgtgtttac agtcgctggg gcgagcttgt
       61 gttcagcacc accgacgtga atcacgaatg ggatggcatg atgcctgatg gcgttacaga
      121 ggcacagcag gatgtgtatt cggttatgat atatgtgaag ggtcttgatg gatctgaaca
      181 tgaatacatc cgtgcaattg cgctgatcag ataattttga atatgcttgt aagaagccgg
      241 ctgcaaagtt tttgtggccg gcttttttgt tgccagattt tttcgtaatg attttgcata
      301 gtgcaatttc tgcagtttcg atatggattt ttgaaaaaaa attcattcgt gagcgaactt
      361 ttaaacgata tatgattatt ataagtgata aaaaagatac ctttggtgtt taaaagatga
      421 aaaacaatca ttattatttc tactttatta aacatttcat gtaataacac atctaatatt
      481 gaatagaaca cctaccaaac ctaataatcg gaagttaaag aagagaaaaa tgaaaataca
      541 atcagttgca gtattattct tgcttacatt atctgttgca ttggatgttc aatgccaaat
      601 tgcacccaac agatatgtca tggaggcatt taccaatgag cagattgagc tcatgtctca
      661 ggaaaggatt gaatatctta attacatcag ccagcatgcc tgggagattg ttgatattcc
      721 tgagggcaag ctgaatgcag attatccgtt attgtaccgg gtggataatg aatcaaaaat
      781 ggctctcaat tctgcattga attgcggaga gcttgattct ttcaatattt tgaatttcaa
      841 atactctata aaaactgaaa ggaattatta ccggattgaa ggatgcagca aatggcttgt
      901 attaaaaagt cacaaggaaa ttacagccgg tttcaacgag tttaaaacaa taaactgatc
      961 tgactatgaa aaaatatatt ttgtttgctg tttttcttat cagttcatca ggtatcattg
     1021 cccagaatta ctcgatgaca acagggacaa taactacatg ttcaggtact ttctacgaca
     1081 gtggtggaaa cggtggaact tactccacaa atgaacttta tacttataca atctgcccat
     1141 ccacccccgg ggccaaggtc tcgctcaatt tcaccagctt caatgttgag gcaaattatg
     1201 acatgctgac gatttatgat gggccgaata ctgcatcacc ttcactgggg tcttatgata
     1261 ataatgttcc attaagcgga gttgttcagg caacaacatc caatgccagt ggctgcctta
     1321 cttttgtttt tgagtcggat ggttcaatac aatatgctgg ctggcaggca gctatcagtt
     1381 gctcgcagcc ttgccagacc attcaggctg ttttctccag ttcgtcacct gctgcatcgg
     1441 gtagttatat caatgtttgt cagggacaga ctgtaagttt ctcgggctcg gcaacttttc
     1501 cgcagaatgg aacaagttat actcaaagcc tggcaacatc cacttttacg tgggattttg
     1561 gagatggcac cactgctacc ggccagaatg ttacccacac gtatgccacc gaaggcggat
     1621 atgatgttga tctggttgtt accgataatc atggttgtat tagttacaat gatattgata
     1681 ttcgtgtcag ggtttcaaca acacctactt tttcaggaac caatgtcagc ccaacttcaa
     1741 tttgtcttgg tcaatcagca acgctgaccg gaagcccgca tatgactccc tggtctcagc
     1801 ccacaggagc tacactggcc ggaacgacct atctgcccga tggtacaggt gtttcatata
     1861 caacgcagct ttgttttact gaattttcgc cgggacaaac tgttactgca gcttcagata
     1921 ttgttagttt gtgtatggac ctggagcatt catactgggg cgatctcact atttcaattg
     1981 catgtccgaa tggcagttcg atgattttga ccgatggata ttcatccaca gcaaccggct
     2041 atgtaaatct tggtgaacct gttgatgatg attattcgtc aacggtcgga gatccttata
     2101 cttattgctt tactccttct gctgccacaa caatggatgc tttggttaca gcaggaccac
     2161 caacatattc ttacaccgat aacgatggaa cggctgtttc tagtatgtcg tatatccccg
     2221 gtggaaatta tgccgccgaa ggtgcatggt caagtttgat tggttgtccg ctcaatggat
     2281 gctgggttat tacagtgacc gatcatcttg gttcggatga tggttcgatt tttcaatggg
     2341 ggataaactt caatcccgca ttgtatccat cgctctgggg atttactcca acgcttgcaa
     2401 cacagcaatg gtcaggtact gccaactcgg gcctcacaac taccaccagc aatccagcta
     2461 cggtaaatcc aacggttgcc ggcaattata cttacactta tactattacc gataattacg
     2521 gctgctctta tgataccacg gtaacgttga atgtttctgc cgggccaaca ataacagcca
     2581 cagcttctcc ttcatcggtg tgccctggtc agagttcaac cctgactgcc agtggtggag
     2641 caacttataa ttggagtact ggtgcaactg gagcttctgt aagtgtgact cctggctcaa
     2701 ctacaacata cactgttaca ggaaccagtg ctgctggttg tattggaaca gcaaccgtta
     2761 cggtaacagc tagtcctcca gcggcaattt ctgcttctgc ctcaccggca gctatatgca
     2821 gtggaactgc gactacagtc tctgcttcgg gtggttctac ttatgtttgg agtccttcag
     2881 gatcaggagc cagttttacg gcttcaccaa ccaccactac cacatatagt gtaacaggaa
     2941 ctacagctgc cggatgcaca ggctcggcga cagttatggt tacgcttaat ccaaatccaa
     3001 cggttacagc ttcggcttct ccagcttctg tttgtccagg tcaggcatca accctgagtg
     3061 ccagcggagc atcaggttat gtctggagtt gtggctcaac aatggtaaat ccgcttgtta
     3121 caacaactta tactgttaca ggtacttctg cagccggttg tacgggaact gcgacagtga
     3181 cagttgtggt gaatccaaat gccactatca ctgcaagcgc tcttccgaca tcaatttgca
     3241 gtgggcagag tgctactcta actgcttcgg gaggagctac ctatgtatgg aataccggat
     3301 caacaatgaa tccgctgaca gtgaacccga catcaaccac atcttatact gtaacaggaa
     3361 ctacagcttt gggctgttca ggaacggctg cgctaaccat aacagtgaac cctttgccgg
     3421 ctgtaagtgc ctctgcatct cctgccaata tatgttttgg ttcgtcctct acattaactg
     3481 gcagtggagc cagcagttat tcctggagca ccggtcaaaa tgcaacttca atatcggtga
     3541 gtcctgttgt aaatacgaca tatactgtaa caggaacttc ttcggcggga tgtacgggta
     3601 cggctactgt ttcaattaat atcagtgcca gtttaagcat ttctgctgct gcttctcctt
     3661 cttctgtctg tcttgggcag agttcgacca tttctgcttc cggagcatca acgtacacct
     3721 ggagtcctgc ggggacagga gccagttttg cagccagccc cacagccaca acaacatata
     3781 gtgttaccgg tagtgatgcc tggggttgta caggtacaac caccgtgaca ttaacagtta
     3841 atccgttgcc gacagttacc gcttcggcaa atcctacagg aatatgtgca ggtcagagtt
     3901 ctgtaattac agctacagga gccaatagtt atgtctggag ttcaggtagc tcaggagcca
     3961 gtttcacagc tacgccttcg gggacaagca cttatagtgt aacggggacg agttccgcgg
     4021 gttgtacagg gtcggcaact gttaccctga ccgttaatcc attgccaaca gtaacagcta
     4081 ctgcaacacc tgcagcaatc tgtgcgggtc agagttcgac gatttcggcc agtggcgcaa
     4141 attcatatgt ctggaataca ggcgcaagta gtgcctcaat ttctgtttcg ccagtgtcaa
     4201 caacaaacta tacggtaaca ggaaccagtg tagcaggttg caccggctca gcaacagttt
     4261 cattgacaat taatccgctg ccggttgttt cagctacagc atcaccatct atgatttgtg
     4321 ccggacaaag ttcaacgatt tcggccagcg gagccaattc atacgtctgg agtccgggcg
     4381 gatctggtgc ttcgttttca gccacacctg gttctacaac aacttatagc gttacaggta
     4441 cttctactgc ggggtgtaca ggaacagcgg ttgtgacact aaccgtcaat ccgctgccga
     4501 cagtgtcagc atctgcatct ccatcagcta tttgtgttgg acaaagttca acgatttcgg
     4561 ccagtggagc caactcttat gtctggagcc cgggtggttc aggtacatct tttactgcaa
     4621 atccgattac aacaactata tatagtgtaa caggaacaac aacggccgga tgtacaggtt
     4681 catcctctgt ttcgttaacg gtaaatccac ttccgaatat tgttgccact gcttctccaa
     4741 tggcagtatg tgcgggtttc agcacaacag tcagtgcgag cggcggaagt tcgtatgttt
     4801 ggagcagtgg acaaactgtg gcatctttca atgaaatgcc ggcagcaact acaacctata
     4861 atgttacagg cacagatgcc cataattgca gtaatacggc aagcgtaacg gttacattga
     4921 tgccaggatt gactgtcaat gtaacccccg aggtagatga catctgtgag ggttcaagta
     4981 caacactgac agcttcgtca ccgggcagtg gtgttggcta tatctgggat acaggagctg
     5041 gttcggcatc catcactgtc agtccgttga caacaacaac atacagtgtt acaggaacag
     5101 atgcacaggg ttgttcgggt acaacaacag ctacagtgaa tgttgatcct ttaccagtag
     5161 caaatttttc tgcagatcct ttgattggtt gcgatccaac aacagttcat ttcacagatc
     5221 tcagcacagg taacataagc atttgggatt gggaattcgg ggatggagat atctccggat
     5281 tgcagcatcc aagtcataca tacacatcgg ggatatataa tgttacattg acagtaacga
     5341 ctgttgccgg ctgtcagtca tcattgacta tgagtaatta tgtaaccatt tctcccaatc
     5401 cagttgcggc atttactgca aatcctgccg ttgtttcgca ggatgaaccg accatcagtt
     5461 ttatcaatca atctacgcag gcgactttat acaattggag ttttggagat aatgcaggga
     5521 atgattatgc tgagaaccca atttatactt attcgggatc tggttcatat gttgttacgc
     5581 tctgggctga aaacgatgcc ggctgtacgg attcagcatc catgactgtc aatgttaagc
     5641 ccacatacac gttctatctg gccaatgctt tcagtccgaa tggtgatgga cgtaatgatg
     5701 ttttacgccc gtttggaaca ggatgggata ctgattctta tagtatgaga atttattccc
     5761 gttggggtga tttggtgttc agcactaccg atatcaatca tggatgggat ggatttatgc
     5821 ctaatggagc cgaggccatt caggatgttt attctgtcat tatttcaatt aggggtatgg
     5881 atgggaagga gaatcattat tataaaggcg ttggtttgat ccgatgataa taaaaaagtc
     5941 tgcatcaata aatcgataca gacttttttt atgtcttgat aaagaattat ccttctttta
     6001 cttcacttct tttttcgttg tcggcttttg gttcttttgg aaacattcgt ttttggaaaa
     6061 cgataatcat gacaaccgca atggaaatat atgaatcggc aaggttgaaa acgggtcgga
     6121 aaaacagaaa atcatcaccg ccaacgaaag gaatccagga tgggtaggat ccatgaagaa
     6181 tgggaaaata gaacatgtca acgacttttc cctgaagaaa tccggcataa ttgcccccaa
     6241 ataatacggc gggattatct cctgacccat aaacagggct cgattcaaat atcatcccat
     6301 aaaacatact gtcgatgatg tttcctgccg caccggcaaa aatgagtgct atggcaaaaa
     6361 tatacagcag atgttttttt tctcgaataa ttttcagcaa ataccacatg atggcagtag
     6421 ctgcgataat tctaaaaata ctcaataaat acttaccgat ggcaccttta agatgcaatc
     6481 cgaaagccat tcctatgttt tcgacaaaat tgagacgaaa ccaatggcca ataacattca
     6541 cttcttctcc caggagaaaa tgtgttttga tgtaaatttt cagaaactga tcgagaataa
     6601 gcaatataaa gataaatatc agtgtattgc gaaggatttt tttattcatc gggcggcaga
     6661 ctgatgcgtg gttccttttt ggtcattttg tttttggcct caatgctgag tgtggcatga
     6721 ggaaccgctt tgagtcgctc tttcgaaatc agttttcctg tcacgcggca aatgccatag
     6781 gtcttgtttt gtattctgac caatgcagct tcgaggtcac ggataaactt ttgctgacgg
     6841 gctgccagct gactgttttc ttccttcgat aatacctggg agccttcttc aagcacttta
     6901 aaggttggac tcgtgtcgct gatgtcattg tcgtttgttc cggtaaaagc ttctgttagc
     6961 aaggccagat ctgcctttgc cttatccagt tttgcaacaa tcagcaagcg gaattcttca
     7021 agttcctcat cgctgtagcg acttttttct ttttcttctt ttgccatagt tcagttttta
     7081 cactttttca acaatagcgt gaactgaaat ctcatcggtc aattctatta aaacggcttc
     7141 agtagcgcct ttcatatccc gaagttcaag ctcagtcgct aatatctcat tgcaaatata
     7201 tgaataattg ttagttattg ccttttctgc taaattcgtc ttatcaacaa aaacacggat
     7261 tttgtcagta acttcgtaat cgcgctcttt gcgcaggttt tgaatgcggt tcacaaactc
     7321 tctggcaacg ccttcctcca gcaatgtctc acttaatgta acatcgagcg ctactgaaag
     7381 acttccttcg ctcatgacaa cccaccccgg aatgtcttca gtaataattt caacatcttc
     7441 caccagcact tctgctgcgc cttcggccaa cgaaacactt attttgcctt cattttccaa
     7501 ttgattgatc tgtgctccgt tcagcgctgc tatggcggct gccagctcct tcatctgctt
     7561 gccgaaacgc ggcccgagct tcttaaaatc agccttgatt ttcttaacca gaaaactgcc
     7621 gtcgtctttg aaaaattcaa gctccttcac attcacctct gccagaatga ttttttgtac
     7681 actctcaatc tgacctttga tcttgttgtc ggtaagcgga atcagaatct tttgaagtgg
     7741 ctgacgtact cggttgccgg tttttttgcg cagcgacaat accatcgatg atattttctg
     7801 cgccagctgc atgcgctctt cgagatcacg gtcaatatat tcgctgttgt atatcggaaa
     7861 gtcgcataaa tgcacgctct caacattgcg tcggccggtc accgaattca gatccgaaaa
     7921 caatctttct gcaaagaagg gcgcaatggg tgaaattaaa accgacaatg tttcaagaac
     7981 atgatacaat gtctggtaag ccgataattt atctttcgac atttcgcctt tccagaagcg
     8041 acgtcgcgat aaacgtacat accagttgct cagaaactca tcggtgaaat tctgaatggc
     8101 gcgtccggcg cgggttggct cgtagtcggc atacgattca tctactaatt taatcagact
     8161 gttcagctcc gataaaatcc agcgatccag ctcgggacgc tcagcaacag gaacttcggt
     8221 atcgctgaaa tcaaacttgt cgatgttcgc atacagcgca aagaaagagt aagtgttata
     8281 cagtgttccg aagaattttc gctgtacttc ggcaacacct tcgctgtcga atttcagatt
     8341 gtcccagggc tgcgaattgg ttatcaggta ccagcgcgtc gcatcgggac cgtatttgcc
     8401 aatggtttca aagggatcaa cggcattgcc gagtcgtttc gacattttgt ttccgttctt
     8461 atcgagcacc agaccgtttg aaacaactgt tttataggcc accgaatcaa acaccatggt
     8521 ggcaatggca tgcagagtga agaaccagcc acgtgtctgg tcaacgcctt cggcaatgaa
     8581 atcggcagga aaactttttt caaaaacgtc tttattttca aatggataat gccactgcgc
     8641 ataaggcatg gagccactat cgaaccatac gtcgatgagg tcgggttcgc ggcgcatcgg
     8701 ttttcctgaa tccgacacaa ggatgatcgg gtcaactgat ggcttgtgca tgtcaaattt
     8761 ttcgtaattg actttgctca tatcaccaac aacaaaatcc ttcatcggat tttctttcat
     8821 gaatccggcc tcgatgctct tgctgatttc ttttgaaagt tcttcgagcg agccgatgca
     8881 cttgatttca ctaccatctt cgctcagcca caccggaagc ggaacgcccc aatagcggct
     8941 gcgactcaga ttccagtcaa ccaggttttc gagccagttg ccaaagcggc ccgtacccgt
     9001 cgattcaggc ttccagttga tggtgttgtt cagctcaatc atgcggtctc gatagtcggt
     9061 ggttttgata aaccatgaat cgagcggata gtacagaatt ggtttgtcag tgcgccagca
     9121 atgcggatag ctgtgttcgt atttttctgc tttgaatgct ttgttttctt tcttcagctt
     9181 gatgacaagt tcgatgtcaa cattcaattc tttttccttt tcaggagtgt aatcgggatg
     9241 atactgcggt ttcacaaact tcccggcgaa ctcgcccatc tcttccgtga atttcccctg
     9301 cttgttcacc atggtgagcg caccgaggtt gtatttcttt cccatgcgga agtcgtcggc
     9361 accaaagctg ggagccagat gcacgatgcc tgtaccatct tctgttgtaa cgaaatctcc
     9421 ggctaccacg cggaaagggt ctccctcagc gggctgcgcg tatggcagca attgttcgaa
     9481 acggatttcg gtgagctctt ttcctttgaa agttccaaga actttgaacg gaatattctt
     9541 ttgtccgggc tcgtaactgt ctatgtcaag tgccgcattc tcggccggaa accagcggtt
     9601 gagcagggga gcgcccacca caacggtctg cggttcgaat gtgtatggat tgtaggtctg
     9661 aactaaattg tattcaatat tttcaccgac agcaagacct gtgtttgacg gcaaagtcca
     9721 tggagtggtc gtccatgcaa ggatatagag attccctttt acaccgggtg tgttgaaaag
     9781 gaattcgctg cgttcgttgc gaaccacttt gaattgtgca acaacggtat tgtcttttac
     9841 atttttgtag caaccgggtt ggttcagttc atgtgtgctg aggccggttc cggcagcagg
     9901 cgagtacggc tgaatggtgt agcctttata cagcagtcct ttgtcgaaca gggttttcag
     9961 cagatgccag accgattcaa tgtatttatt gtcgaaagtg atgtatggat tttcgaggtc
    10021 aacccaatat cccatgcggc gtgtaagctc gtcccactga tctttgtatt tcatcacttc
    10081 ctgacggcat tcgcggttgt agtcaataac agaaatgctt ttaccgatat cttctttggt
    10141 aatcccgagt tttttctcaa cgccgatttc caccggaagt ccatgtgtat cccagccggc
    10201 cttgcgtttt acctgaaagc ctttcagtgt ctgataacgg cagaaaagat ccttgatggc
    10261 gcgggccatt acgtggtgaa tgcccggcat tccattcgca ctcggcggtc cttcgaaaaa
    10321 tacgtattcg ggtttgcctg cgcgcgtttc aatacttttg cggaagatat ctttcgaatc
    10381 ccaccattgt cttacatcat cagccagttc tgtcagcctt aattgcttgt actcgttgta
    10441 tttgcccttc attccaaagt gatttttaaa aagaggtgca aaagtacaaa ataatgtgct
    10501 gatttgctaa tgtgctgatg tgctaatttg caaattgtgc attgtgcccc atgctctatg
    10561 cgttgcgctt tgtgccttgt gcattgagcc tcatgctttg cattttcaga ttttttcgta
    10621 acttgcacat tctttatgca ttaacgtatg tctttcaagt catttatttt tgcaatagta
    10681 ttttttatat cagtaggcgg actgatggtc gcagacatga ttgcgcctca ttcttcacat
    10741 acacccgatc ctataaaact gcgcgatttc gatgagattg ttgcatctga tactcttcat
    10801 gtaataatgg gatacaactc tgtaaattat tttgtgtaca aaggaacacc gatgggttat
    10861 cagtatgaaa tggtgcagca attatgcaaa gatatggggc tgtttctcga tctgactgtt
    10921 aataacgatg tagaagaagc gtttcgctgt gcggcttttg gtaaatgcga tttgatagcc
    10981 atggaactca atgttgcaaa aaatcggttt ccacgaattg tctttacaga ttcattgtat
    11041 aaaatgcgct ccattcttgt gcaacgaaaa gatagtgcgg gattagtgca ggtgaaagat
    11101 ctcaaacatc tgagtggcaa aactgttctg attcccagga aaactattta ccaaagacaa
    11161 ttactcgagc ttgaagatag tctgcatatc gatattgata ttgtgccact tggagaagtt
    11221 ggaactgaag atttatgtaa tctggttgcg gaaggcaggg tggattatac catttgcgat
    11281 gagcatatgg cgcggacttt atgggcctat tatcctgaac tcgattttag tgtgattgtt
    11341 tccaaacctc aaccggttgc ttgggctgtc aatagcaatt cacaagtttg gctttcaaag
    11401 ctcaattcat ggattgaaac ttataagaaa aaagggaatc ttcattttct tcaacgtaaa
    11461 tattttgatc atccggtttt gtatcttttt ggtatgccgc atgaacacag tctttccggt
    11521 ggaaaaatca gcaagtatga tgatctgata caaaagtaca gcaacgaaat cggttgggac
    11581 tggcgcctgg ttgcttcttt gatttatcag gaatcgcgct ttacaacaga ctttcagtcg
    11641 ccaagtggtg cttttggttt aatgcaactg atgccaggta ctgctcggca tatgggtgtt
    11701 aattataaca gcagtgcgga agaacagtta aagggtggtc tgaaattgtt gcattatatg
    11761 gataccgcct ttgccattta tattgatgat gaatcagagc gtcataaggt tgtgattgct
    11821 gcttacaatt tgggcatggc acatttttac gatgcatttg agttagcgaa aaaacatggc
    11881 aaatccccaa tcacttatgc tgttgtgatt gaatgcttga aatgcaaatc aaagccatta
    11941 tattacaacg atccggttgt aaaatttggc taccttaacc cttggtatgt cgagcgtttt
    12001 gtacgtgaaa tttatgcgcg ctacaacgca tactgcgcgg tgtttccgga gaaataattt
    12061 taaaggctgt cgcttgttga atgctacacc atgaattcat caatccatca agtttattaa
    12121 gtagaaaggc gatgtggtag tagtcagtag ccaatggtca gttgtcagtt ttattttcac
    12181 ttcaaccttg ctttcaaact tcacttcttt cttcaaaatt ctttaaatca tcaatcagat
    12241 caaaaatcac aaattttcat cttgagcttg tcgaagggca cctatcggta gtcttaaatc
    12301 aaaaacctcc ttcttacttc gatgttcctt gttctctgtt ccgtgttcaa aagggcttac
    12361 acatacaaat aatccgtatt catccggata tatctgttca gtgccgacca cgacgaaatc
    12421 caggaaataa acatgccact cagaatgatg gctccgaata gcatatatac ctgactgtca
    12481 tcctgtatgg cctgcagatc gggaatctgc tgataggcga gcatcatcac accttcgagt
    12541 aaaacaacgg ccagcagact gcttacaagt cccatccaca aggcctgaaa cagaaacgga
    12601 cgtcggatga atccgcgtgt agcgcccacc agcagcatgc ttcggatgag aaaacgtttt
    12661 gaataaatgg ctaaacgaat agtattgctg atgagactat acgatataag gaagaccagg
    12721 agcgcgaatc cgagcagtat taatgaaact tttcctgaat tcacattgat gtcttcaaca
    12781 agatctttct gataatatac atttccaaca attgattctt tctgcacttt tgccacaaat
    12841 gcatcaatgg cttcactggt tgcaaagtca ggtttgagtg taatatcgag tgatggcgga
    12901 atcgggataa taccaaggaa ctccacaaat tcttcgccgg tttcttcggt catgcgtttg
    12961 gcagcttctt cggaactcac catggagcat gtttgtacgg ccgggtcaag ttgcattttt
    13021 ttctgcatgg ccagcatgtc ggcttcgggc agactgtctt tgaacatcac cgtcacgatg
    13081 atgttttctt ttacgtagtt tgaaagccgt tttgaatgca gcagaataag tcccgacagt
    13141 ccgagaatga gcagtaccag cgaaatgctg atgatcaggg tggcatagga gcccccgagc
    13201 cggatggaag atttgcggtg ttttgccatg acgcaaaaat agtattttct ttgcgaatgc
    13261 agacatcttg tgaattgtgg gttgtatctt tttgtaacta ttgaaattaa atgaaaacgc
    13321 acaaagtttt tgcagaaaaa cagagaaaat catataagtt cccggtcacc aattcaaaac
    13381 agcatctgcg gcctgtgccg ggttcttgcc gaaaaccttc aaagcttttt ctctgtttct
    13441 tcccgaggtc tgccttgtca gtcatataaa attccacgtc agcaatgctg cgaagtcaac
    13501 tttttcttgc tgacggtaga atgtcgggag agacccaaca ttctgtcgtc cgtactacaa
    13561 tgatttgaga aaaatagcgg acacggaatc gttgggtttc acgaatatat acttgaaaat
    13621 gttgtttgta ttgcaaaaat tgatttgtcc gtttttgagt ttgtgaaagt aaaactattt
    13681 cttcaccacc cgtatctcaa cccgcctgtt catctgctgt tcacttgcat ttctttcttc
    13741 ggtaagaggc ctggtgtgtc cgaatccttt ccacgtcatg cgctcggccg ctatcccttt
    13801 gcttatcaga aaatcataaa tagctttcgc tctttgtcct gacaaatctt cctcatcatt
    13861 tcgcgaacaa caaatgtgac cttcaatgga aatttcaagc gtgggatttt ccgccatcac
    13921 ctgataaagt tcatccaata ccggccttga aactggcaga ggaattggcg ttccgccaat
    13981 aaaggaaata ttttccaatc tgatcaggtc acccgcctct gcttgtatga ttttttcctt
    14041 caatacagac attttctttt cctcaggtct gctggttaca atatccacgc gccggttgcg
    14101 aaaaaattct gtgtcgccaa attccagcgt ttcgtttttc ccggctgcca gtgtgctttt
    14161 tacatcaatc cctttttgaa caaatactga tttcacagca ttggcgcgcc tttccgacag
    14221 cacctgattt cgttcagttg tcccgactga atcgcaataa ccgctcagac tcacaatcga
    14281 cgctttatat tttttgcaat aggccgccac cgtatccaga atccgcatgg ccgctgcatc
    14341 cggctcaaac gaagctgtgg agaaataaac actgtgtgtt tttgtttcct gcgctggtaa
    14401 tgatgcaaag gcaatgattg ccgataaaaa gataatgact gatttcatgg ttaaaatatt
    14461 taatgattaa attaacgcaa aattaattgt gtttattgca ttaaaaatat ttttccgatt
    14521 tatttcttca ccacccttat ctcaacccgc ctgttctcct gcttttcggc ttcggtcttt
    14581 tcttcattca atggccgtgt gtgcccgaat cctttaaatg acatgcgttt cgcttcaatg
    14641 ccttttttta taagataagc atacacaacg taggcgcgct cgccggagag attggaaaag
    14701 tcatcactga tgcagcagat atggccttca atggcgattt ccagcgtggg ctgcgacagc
    14761 atgatgttgt atagctcttc aagaaccggc atcgattcag gcataggacg gctggagttg
    14821 ttgtagaaac ttatattttc aagacggatc agatcgtcga cctgtgcctg tgaaatcttt
    14881 tcttccagaa tagataattt cttttcttca ggtctgctgg tcacaatatc cacgcgtcgg
    14941 ttgcgaaaaa aatcagtacc gtcatattcc agtgtttcgt tttttccggc taccagtgcg
    15001 ctttttacat cgattccttt ttggataaat attactttca ctgcattggc gcgccattcc
    15061 gacagtatct gattgcgttc cgatgttccg accgaatcgc aataaccact cagactaacg
    15121 accgtagcat tgtgtttttt acaataggcc gccactgaat ccagaacacg catggccgct
    15181 gcatccggat gaaaagatgc tgtgctgaaa taaacactgt gcgtctttgt ttcctgcgcc
    15241 attgccggtg aaagcaaaat aaataaaagc aaagtaaata atgtgtgttt catgataatt
    15301 aagctttgta tttaattctc aacggttgaa ttccttattt tattgctctc attctttcaa
    15361 gacaaaagat aaaagtacaa agataaaagt gaccatctaa tattcccata tcggcgaacc
    15421 cgtcacagct tgccaagttt tacgtgagcc tcccatttcc aaatcttttc cagaattatt
    15481 tatttccaca tacgccgact tgtcacgttt tacgtgaagg cgtcacataa gcgaagcgga
    15541 atttaatccg cagaaggcgg atcatttcca catcatattt cgtatttctg atttcgtatt
    15601 tcgtattttc tgcaggatat tgaatttcca catttccaca tcatattaag ataaaagaca
    15661 aaagtacaaa gataaaagtg attctctcat attcacacat tcgcgaagcg acacatcgac
    15721 gaagtcgcac attttcacat ccgttcatat ttcgtatttc gtatttctta tctcgtattt
    15781 ccttactttt gccccatgca acctatcatc ctttggcagg atcaatatgc acttgcagtg
    15841 aacaagccca acaacgtgct tgttcatcat tccgattatt ccggcatcga aggagaaaag
    15901 tcgttgcttg aattattgaa ggagcaggga ataccccgcg tatatccgct ccaccggctc
    15961 gacagaaaaa catccggcat tgtgctgctt gcaaaacacc ggagcaacgt tgctgttttt
    16021 cagaaattat ttgatgaaca gaaaattcga aaaacctatc tggcactgct tcgcggacat
    16081 gtcgatgcag aagggatagt tgatacccct gtgcgcaacg atcgtggaaa ttatcgcgat
    16141 gcattaacgc attaccgctg cattgagcat tatgagctga atgtagctgt tgagccctac
    16201 gacacatcac ggtacagttt ggttgaaatg gaacccaaaa ccggacgctt acatcagttg
    16261 cgcgtgcatg ccaataaaat cgcacatccc attattggcg atcacaagca cggaaaccgg
    16321 caccacaaca ggcttttcgc tgaacagttc ctgttgccgg atttatttct gcatgccagt
    16381 gaattgcaat ttacacatcc gtttaccaat gaagaaatca tcatcaatgc tctacttccg
    16441 gctttctggg cgcgcttcga tgaaatcaga aaagaactga gtatctgatc acaccctgaa
    16501 gctcggccac taaggctcaa agttacttca atggattttg ccacaaagac actgaaaatc
    16561 accacctgct aatatacatg gtttcaaaaa atattttgtg acttagtgtc tcagtggcat
    16621 cttcatgtac cataatctga atcaggtttc accgcaggcc tgcctgttgc agaaaggtga
    16681 agactgattc gaacttgccg cccatcacgt cacgccagtg actgatgccg gcgggttagc
    16741 gttcataaga cctgctaagg ttttcgatga gatcccggct ctgcacaagc taattcaata
    16801 atgagcggat tccttttccg gccgggaact aagtagtaaa aagttgtcaa gtttttaaag
    16861 ttgaaagaga cttcattaaa ttcgaacttt atttcatatt tccttgcctg cctgttgcag
    16921 acaggttcga tgttcatgat tcagtgttca aattttgtgt cttagtgcct tcgtggcaat
    16981 ctccaatgat attcaataaa ttagtatttt tgcaaatcaa aatcaatccg aaaatgaaag
    17041 cactcatcaa taccgtgaag ggcaccatga aagtcgaatt ctacgaaaac gacgccccga
    17101 ataccgttgc caactttgta aagctcgcca aaagcggctt ctacgatggt cttactttcc
    17161 accgcgtcat tcccgatttc gttattcagg gcggttgccc tgacggaacc ggtgccggtg
    17221 gcccgggcta cagcatcaaa tgcgagacca gtggcggcaa tcagtatcac gatcgcggcg
    17281 ttctttccat ggctcatgcc ggaaaagata ccggtggatc acaattcttt atctgtcaca
    17341 gccgcaccaa cacagcgcat ctcgaccgcc gtcacacctg cttcggcaaa gtagtcgaag
    17401 gcctcgaagt catcgacgcc atccgccagg gcgatgaaat tgaaagtata aagattattg
    17461 aagaatagcg cacagctgcg ttttcagatt ttgtgttttc tatctcgctg agaactctta
    17521 gttgttttct tcgtaatagt aggaataatc aattcctttc aaaaacatgt cgcggtcatt
    17581 aatcttgccg gtgagggcgt tttttaaaag tgcttttaat atgctgctgt ttgtcgggct
    17641 gagcatcata gcgttcatgt aatcgcgctt gctgatttta ctccagtcaa cacatttttt
    17701 cagacgtttt ttcagcatca gatccaacca tatacgggtg cttctgccat tgccttccat
    17761 aaaaggatga gcaatattca tttcgatata tttgtctgca atttcttcaa atgttgtttc
    17821 cggcatggat tcaatctgct tcagtgtttc gtccagaaag cgcgctacgg caaaatgaaa
    17881 accacctttc gaaatatttt tttgtctgat ttgcccggca aaatcataca agccgccgaa
    17941 caagtacgcg tgtatctgct gcaaacctct ggcagtgcca acttcgatgc tatcaatgaa
    18001 tgagctttta aacaaagcat acgcttttgt tttgcttttt ccatcgatgc tatcatcgct
    18061 gtaagtaaac caatcgataa accggtttgc ttttttgccc ggaaattctt tacctaaggc
    18121 aatgataccg tcgtaatcca gcatgtcagc taaacgcttt ttgccgtcag gggcaagaaa
    18181 tttcaactgg gtagtgttac taaccacttg actattttct ttctttaatt tggctttgag
    18241 atatttccaa tagttacgat tttttgagta gtcatcttga tttgtcagca ctgcaataat
    18301 atccaataca ctaaaccacc accgggcgtt ttgctcgtcc cagacagccc gcacttcgcg
    18361 gtcatcgaaa aatcgtatgg atatttttga ctgactcatt ttatttgttt caacaattcc
    18421 taagcccgca attcattggg aaattcaaat gtatgaaatt atttttgggt ggggaaaaac
    18481 agggcaaacc ggtgaagttt ttttacatct tttgacagat tagacagttc cgttttttct
    18541 atcattttat tgattgttta atagccaatc tattgcattt atctgtttta ctccgttatg
    18601 acttcctgtt tcgtaatcca tagtaataag tatccgctcg ttataatcgg gaattctgtt
    18661 aaacggtgca agttcgcgct caagtgtttg gagttccctt acggtatatg caacctggat
    18721 atattgcgta taaccatctt tgtcccggac aacaaaatca acttcaagat tattcgtttt
    18781 tcctatccag acctggtaat ttcggcgcag taattcaaga aaaattacat tttcgagtat
    18841 atgcccggca tctgctgcca gttctttccc aagcaaggca ggtcgcaagc ccaaatcaac
    18901 caaatagtac ttttcctgcg ttgccaaatg ctgttttcct ttaatatcat atctgcttat
    18961 tttgtaaaaa agaaatgatt caacaagcgt atcgatgtat tttgacaccg ttttattgtc
    19021 tatcttttta ccgtttgctg ttaggattct tgcaatattc cttgccgaaa cagttgaacc
    19081 gacattgtca atcagaaaat gaacaacctc gttgtatgag gtttcataat agatggtgtg
    19141 ccgtttttgt atatcatttt gatatatgtt ttgaaaaacc gttttcaggt attcataagt
    19201 aaagttttca ccagatccgg ccaatccgac agcttcagga aaaccgccta ttcgcatata
    19261 ctcggcaaat gcgcgatccg ggcttgattc tttgcctgta aattccagat attcggcaaa
    19321 agaaaatggc agaatattaa tttcaaatgc cctgcctgta agtatggttg ccaactcgct
    19381 tgatagcaga taagcatttg agccagtaac atacaaatct gtatttggtg tcacaaacag
    19441 cccttcaagc agtttttcaa ataccgggat attctggatc tcatcaataa agacataatt
    19501 cataacatct ttttgcaaca tcccgttgat atgatcgtaa atttctttcc aatttttttc
    19561 ggcaagaaac cgaaattccg gtaaatccat attgatggaa atgatcgaca cattcggatg
    19621 ttcctttttc agtaattcct gaaactgtaa aagcaatgtc gacttccctg cacgacggac
    19681 acctgtcaca acttttatca gattcttgtc tttaaggatt gaaagtttgt agagctccgc
    19741 ctttcttaaa attttttgca tacaagtcgg ttttgaatct tacggagcaa atataaacaa
    19801 aattcctcaa aatataaaat agtgtaatgt ttgaggaatt tcgccattcg ccagggcgac
    19861 gaaattgaaa gcattaaaat tgttgaagaa tagcgcgcag cgccggtttc agattttgtc
    19921 gatgattatc tgttactgct attacagcca gattacagca aagtaattct gttgtaatag
    19981 ttttgtgtgg agaatacttt tcaatgcctg cataaaaact actttaacat tggcgtagaa
    20041 cgttcccggt caatgtatga gatatagctt ctgcttcggt gccgggatct cctcgaaaac
    20101 tatttaagcc cttgctgaat ctacatttca ggagaccccc cgacggtacc gtctggggga
    20161 cttggacgtc taaaccgggt aagcacagcc acaacatcaa tcacactaaa ccaccaccgg
    20221 gcgttttgct cgtcccagac ggcccgcact tcgcggtcat cgaaaaaacg tatggatatt
    20281 tttgactgac tcataatacc ggtgtctctg ttatttcagt ttcttcattg aacggcttgc
    20341 aagtaaggat ttcatttgtg taatagcaac tttgttaagt tggattagtc tttcgctctg
    20401 agataagcct tgtcggatta gcagtgcgtt tatgctttcc atgttgctca atacaacaag
    20461 ttgctccaag gtggcataat ccctgatgtt tccgttttta tcaaggttgc gttcccgcca
    20521 ttcttttgct gtaattccga aaagggccac attcagaaga tcagcttcgt tagcatacac
    20581 taaacttgct tgttgtttgg ttatctctgg tggaattaaa ttgtctttaa tggcatcagt
    20641 atgaatttcg tagtttactt tagcaagggt tcgttgtatg ttccattcta acttaagacg
    20701 atcgttttca tcttctttca gacgctggta ttcttttacc aaaagcaatt gaaattttgg
    20761 gctgatccac attccaaaat gaaatgcgat gtctttgtgg gcatacgttc caccgtatct
    20821 tccaggctgt gctgtaatgc caatggcgtt agtggcttca atccaatttt taaccgaaag
    20881 aacaaaatta ttgctccctg ccgaattttt aattgtaccg aattcggtat aattaaaatc
    20941 tgtattgtac aacgcttccc attcgccgat atattcaatg gtgcttttca gactgagcca
    21001 tttaataata atggcttcct gcatttgact ttttgccata tcggtaagcg aaatgtaatc
    21061 gtcctgcttt tgggatatga ttgtaatttc agttcccccg atttctattt ttctattttt
    21121 cgccatatta ataccggttt cgtcaattct ttttcgctgt catattccgt gtaatttttt
    21181 tcagtgtgta ttatatcctt gctggcacta gccattgccc ccctatccct aagcatgcaa
    21241 ttcaatggga aaatcaaatg tacgaaatta ttttggagta agatcgatat ctgaaacacg
    21301 cagaattatt ttgtacttgt ttgattttgg ccgagtcgct tcccaatttt ttattggctt
    21361 ccaagtagat tactctatta atcagattat tttaataaat ctccagagtg tttatcattt
    21421 tgaaaaagtc attttttgtt ttctcaaaat ccatttctaa tgacaaacaa attatgtaat
    21481 ataaaacatc attatcaact attaaaaaag caagatctct gaaattgaaa ttgaaatctc
    21541 gatttttaaa atctacccat cttacagttt tatcattaat aattaaaact ccagtcccca
    21601 ttacttcata attattaaat gaattgggaa aatcttcaat tacccatgaa ttccatattt
    21661 catctagagt tttaccatcg gcagggaaag ttgctatttg aatgttttcc gaatataaat
    21721 ctaaagcacc ttgacttgat tgagaaaaaa ctaaagaact atcttctgaa tgattttccc
    21781 aattctcaat gggaataata gagaatttat tatttgaatt aaaaaccctt tgttgtccat
    21841 aagcgaatac tccagagact aacaataaaa aaattaaaaa tatagatctc atttttcaac
    21901 agtttggttg gagaattaga ctcaattagt catttacgaa caaaaataca aaaattgatg
    21961 caataaataa atttgtattt tttgtctatt aagattctgt aagtcccttt tgtagcgctt
    22021 tttaaatttg taaggataac tgaaattatt ttttggcaat tcagatttgt cattggcagg
    22081 caccgtattt taatctattt cttctagttc cagcaaaata ctattgtctg acaaaaatga
    22141 tttgaacaaa ttgtagtcat caaactcaat agtgtttgct ttcacggttt gtgcgtcagg
    22201 aatacattca tatttattct tgattaccca atatctgata ttggttttat ctcgcttatt
    22261 ttctgacttt gcaataatcc attcagaatt ataagcaatt tccattactg tcattgggac
    22321 aatttccact cctgatgacg cacagctttt ttcctgataa agtatttgtc tatccacgtt
    22381 gtcaaattca gacaggtata ggttattgcc caaatattct tcttttgctc caaaacaaca
    22441 ggattgaaga attacagcaa gagcaataac agaaagcaat aaatatattt tcaattccag
    22501 tttcatttca aatattctta ggtcacttcc gattttcccc aatagacgaa attagtcaat
    22561 cctggcccta tctgctcatc cacaaatgta taaacatttt gacaaaacca tcaacccatg
    22621 cattttcaaa ttttcaaccg aacgatgaaa tcgtgagctc gacttgtcac agcttgcagg
    22681 tttttacacg gtttccacgt taagtcgcac attggaattt attccgctgt aagtggaaca
    22741 tcatttcgta attctgatct cgtattatct atgttttttt taaggtgctt cgcgtgttct
    22801 ttgtccctgg ggctatcgcc caaggctaat attgttcgat cagcgtccgc cgatcgcttc
    22861 aaaggaatca actatggtaa tttttgggat aaacgttttt ataaacacaa actcctcact
    22921 tcttacgcct gatgacttac gccttatgcc tatctcgtat ttcgcatctc gtatttctca
    22981 tctcgtattt cgtatctcgt attttcgtat ttttgcacct caatttttca accatggaaa
    23041 ttccaacaaa atacgacccg accaaaagcg aggataaatg gtacgcctac tggatgaaca
    23101 acggcttctt ctgctctgtt cctgatgatc gcaagccata caccattgta atccccccgc
    23161 ccaatgtcac cggcgtgctc cacatgggac acatgctcaa caacacgctg caggatgtgc
    23221 tggtgcgccg cgcccgtatg aaaggcttca acgcctgttg ggttcccggt accgatcatg
    23281 catcgattgc caccgaagcc aaagtggttg gaaaactcag ggctgaaggc attgacaaga
    23341 aatcactgtc tcgtgacgaa ttcctgaaac acgcctgggt ttggaaggaa aaacacggcg
    23401 gcatcattct cgaacagctt aaaaagcttg gcgcatcatg cgattgggac cgcaccgctt
    23461 ttaccatgga tcagaatctg tacgattcgg ttataaaagt gtttgtcgat ttatacggaa
    23521 aaggcctcat ctacaaaggt caccgcatgg tgaactggga cccgagcgca ctcacagccg
    23581 tcagcgacga ggaagtgatt tataaagagc agaacgcaaa actctattat ctcagatatt
    23641 atctcgaaaa cagcaaggaa tatctggtga ttgccaccac gcgtcccgag acgattctgg
    23701 ctgatactgc ggtttgtgtg aatccgaacg atgaacgttt caaacatctg cacggaaaga
    23761 aagtgcttgt acccctcatc aaccgcgtga ttccggtgat caccgatgaa tatgtcgaca
    23821 tggaattcgg tacgggttgt ttgaaaatca cgcctgctca cgatatcaac gactacacca
    23881 ttggacagaa attcggtctc gaagtaattg acattctcac tcccgatgga aaattgaatg
    23941 agaaagcgca gttgctgatt ggtgaagatc gctttgcagc acgcaagaag atcatgccga
    24001 tgctcgaaga agcaggcaat gtcgaaaaga ttgaagacta cgtaaacaaa gttggataca
    24061 gcgaacgcac cgatgttgcc atcgagccga ggctgagcga acagtggttc atgaaaatga
    24121 gcgacattgc aaaacccgca ctggatgcgg taatgaacga tcaggtgcag tttcatccgg
    24181 ccaagtttaa aaatacctat cgccactgga tggaaaatgt gcgtgactgg tgtatctcgc
    24241 gccagctgtg gtggggacag cgcattcccg catggtatct gcccgacggc aaagtggtgg
    24301 ttgctctgaa tgagaacgaa gcacttgaaa aagcgcatca gatcaatcct gcgttgaaac
    24361 tgtccgacct taaacaggat gaagatgtgc tcgatacctg gttcagcagc tggctctggc
    24421 ccatcagcgt tttcaacgga atcatggagc ccgacaataa agacatcaaa tattactacc
    24481 caaccaacga tctgatcacg gcacctgaaa tcatgttttt ctgggtggca cgcatggtta
    24541 tggccggact tgaatacaga aaagaaattc cgttcagcaa tgtatatttc accggcatcg
    24601 tgcgcgacaa gcagggacgc aagatgagca agtcgctcgg caacagtccc gatccgatcg
    24661 agctgatgca gaaattcggc gctgatggcg tccgtgtagg catgatgttc agctcacctg
    24721 cagggaacga tcttttatac gacgattcac tgtgcgagca gggacgcaat tttgccaata
    24781 aaatctggaa tgcattccgt ctgatcagtg gctggcaggt cgatgaaaaa ttgtcgcagc
    24841 ccgcttctgc agcgctttcg acagaatggt tcggacatcg tttgcgtcag actttgaagc
    24901 agattgatga gcagtttgtc gatttccgca tctccgatgc attgatgtcg gcttacaaac
    24961 tggtgtggga cgatttctgt tcctggtatc tcgaactcat caaacccgca tatggtcagc
    25021 ccatcgatgg aaagaccttt gcggaaacca tggaatattt cgatcagctc atgcgcatgc
    25081 tccatccttt catgccgttc ctgaccgaag aaatctggca gaccatgaag cagcgcggcg
    25141 aaaaggaatc catcatgata cagccgatgc catctgattt caatcacgat gaaaagctga
    25201 ttgccggttt tgaagtggtg caggaaacca tcatggccat ccgcgcatac cgcaacgaca
    25261 aaggccttgg catgaaagag ctgctcgatg ttttttatac cccggtacag catggtgaaa
    25321 caatacagaa gtacgctccg gtgataatga aactcggcaa cctgagccgc tttgagaagc
    25381 tgagtgaaaa gcccgaaaaa tcatttaccg ttcttgtccg caccgaagag cttgtatttc
    25441 cggtgagtgc cgatctcgat accgctgccg aaaaagaaaa gctgatgaaa gaacttgatt
    25501 ataccaaagg atttctggaa acagtccgca aaaaactgtc gaacgaaaaa ttcgtcagtg
    25561 gcgcaccggc tcaggtaatc gacaacgagc gcaaaaagga gcaggatgcg ttgtctaaaa
    25621 ttgcggttct tgaaaagaca ctcagcgagc tctgagattt tttcagatta aagaattaag
    25681 tcatatctgg catgctgaca gatgttttgt atattgttct cgctctgttt ctgttgtttt
    25741 tcggagcaca gtggctggta acgggcagtg caaaacttgc cgaggcatat ggtattccgc
    25801 aattgattat cgggctgacc attgttgcat tcggaacaag ttcgccggaa ctgattgtca
    25861 caattacagc atcatcacag ggacagaccg gtatggccat aggaaatgtg gttggttcaa
    25921 acattatgaa tattctactg atactaggtc tgacagcagt tgtcagtcct gtatttgtgg
    25981 agaaaaagat tatccggtcc gacattcctt tgctgatcgt gttttcctct ttgctgattt
    26041 ggatactgat taatcctgag ctgaaaagtt gggaagggtt cattctgctt ggttgtcttt
    26101 tgctgtatgc cggatggatg tttttcaaca tcagaaaaaa acggcagttg ctgaaagcag
    26161 atggaggagg gaagtcagca acgcattttt caaaaaaaca gaaatatttt ttcgtatttc
    26221 aggtttttgt aggtttaggc cttctcacag caggatcccg tttacttgtt ctgggggcag
    26281 tcgatattgc ccggctgatg aatgtgagtg agactatcat tggactgact gtagtcagta
    26341 taggtacagg tcttcccgag ttaggcactt ctgctattgc tgccatgagg aaacaatcgg
    26401 atattgctgt tggcaatatt gtgggatcaa atattttcaa tattctggtg attgccggca
    26461 tttccatcag tgcatatcca gctgccgaca gcggtatcag tctgattgat ttgcttttca
    26521 tgatcggagc ggctgtgttg cttttcccgc tggcgtatta cggttcggcc attcgcaggg
    26581 ctgaaggagc tttgctcttg gggctttatg tgctttatct gtttatcatc tggccataaa
    26641 gaatctgtaa ttcagaaaaa tttgattttt ttcgcaaagt aaagattaac atggcccgat
    26701 aatacaatag tttattgcaa ttgccgttta ttcactgatt tttattaatt ttgcacccat
    26761 gaaattttgg cggaattttg ttcttattgt tctcattctc actggggttt cctgcagcga
    26821 gtatgacaag ttgctccggg aaggaactcc tcaggctcgc ctggaggcag ctaatgaata
    26881 ttacagcaaa ggaaattact tcaaagcttt gcagctgtat gatcagttga ttgttgaatt
    26941 acgcggcatg ccgcagttcg aagaggccta ttacaaatat tgctggagca actatcagca
    27001 ggagcagtat ctggccgccg cttactattt taaaaacctg gctaaaactt tacccaacag
    27061 taaatatgct gaagagtcat tgtacatggc ggcatattgt cagtatcttg aatcagccga
    27121 tgcggctttg gatcagacat cgacgaacaa cgcactgacc gagttgcagc tatttgtcaa
    27181 taaatatcca aaaagcgaaa agattccaca atgcaatgaa ttgatggatg aattgcgtta
    27241 taagctggaa atgaaggatt ttaatactgc gatgttgtat tttaaaattg aagaatataa
    27301 agcggctgtt gtcgcattca caaatctgct taaggaatat cctgcaacaa aatttaagga
    27361 acaggccctg ttttatattc tgaaggctga atattattat gcttcaggga gtatcgactc
    27421 aaaaaagaaa gaacggtttg agcatgctgc aaaagcttat accactcttg tactgcagtt
    27481 tcccacaact tcatacaaga aggaagcgga tgtgataaac gaaaaaatca aagtgcaaat
    27541 cagtaaactg aattaatata tggattacaa gaaactgaaa atcgaaacca atgctgtaac
    27601 ccgcaacctg catgagcttg agaatgagac cggaagtatt tacgaaagca tcatgatcat
    27661 agccaagcgt gcaaatcaga tttcaatggt tatcaaagag gatctccagc atgaatcgga
    27721 gcagtttgtt tcgactatgg ataatctgga agaagttttc gaaaaccgcg aacagattga
    27781 acttgcaaag ttgtacgaag ctcttccaaa accaacatta attgcgttgc acgaattcca
    27841 gcagaaaaaa gtatatcatc gtcgcggcac acaggaaaac taattctgat tgaaaaattt
    27901 cagggtcata cttggtgttt caggcggaat tgcagcttat aaggcgccgt ttattatccg
    27961 cctgctgaag caaaaaggag cttcggttca ggtggtgtgc actcccaatg ctcttgaatt
    28021 tgttactttg ccggtactcg aaaccctttc cgaaaacagg gtttatattg atatgtttcc
    28081 gaaaacttac caacagtcgg ctgagcatat ttccataacc gacaatgccg atatcatgct
    28141 tgtggcgccg gctaccggaa atgtaattgg taaatttgca tccggcattg ccgacgatgc
    28201 gcttactacc tcgtttatgg ctttcgacgg tccggtattt attgctccgg ccatgaatac
    28261 aaaaatgtgg aattctccgg ttgtgcagaa aaatgttgct tatctgaaat cgctcggtca
    28321 tacaattatt ggcccggctg ccggcgattt ggcttgtggg tataagggct ccggccggat
    28381 gcttgaaccg cacgaaattg tggatgaggt gcttggttca cttgcatcgt caacacttgg
    28441 tggtcaaaga attttggtaa cagccggccc gaccttcgaa aggattgatc cggtgcgcta
    28501 tctcggaaat ttcagcacgg gacgtatggg gtatgctgtt gcttcggcgc ttgcagcgcg
    28561 gggtgcagaa gttgttttag tctccggtcc ggttcatttg caggccgatc cgcgcgtgaa
    28621 gctcattcag gttgaaagcg ctcaacaaat gcacaatgtc tgtgtgaaac attttccgaa
    28681 atgtcaggcg gctgttatga cggctgctgt agccgatttc cgccctaaaa ccagcgcgaa
    28741 atcgaaaatt aaaaaggaac agaatgcttc gttaaatatt gaactggagt tgaatcccga
    28801 tattcttgcc accattggtg gaatgaaaaa agcggggcag attgttattg gttttgcact
    28861 cgaaacaacc aatgaattag cgaatgcgca aaagaagtta gacaataaaa aggccgatat
    28921 catcgtttta aactctctga atgatccggg cagtggattt ggacatgata caaacaaaat
    28981 aactttaatc agaaagtctg gaaaacccca gccatggccg ttgatgagta aaaccgaagc
    29041 tgcatcggtg attgcagatg aactggaaaa tcttctatta cgaaagaaat gagagcgatt
    29101 ttaatactta gttttctttt gcttttttca tcagtaaatg cgcaggaact catgtgcaat
    29161 gtgcaggtga actccagtca ggtgcagtcg agcgataaaa cggtgttcga aaatctgcag
    29221 actgcggtgt atgaatttat gaataacaag cgctggagca attatgaatt ccgcatggaa
    29281 gagaaaatcg aatgctctat tctgctcacc attagttcct gggacaatat tgaatcattc
    29341 accggcacca ttcaggttca ggcgcgcaga ccggtgtacg gatcatccta cagtacaccg
    29401 cttttcaatt ttctggataa ggatttttca ttcaaatata tttcggggca gccacttgat
    29461 tatattgaaa atacttatac ttccaatttg acttctgtgc ttgcattcta tgcttatctt
    29521 gttctgggtt tcgattttga ctcatttgaa gaattgggcg gcactccttt ttttgaaaag
    29581 gcccagggtg ttgtaaatgc agctcagagc gctcccgata aaggttggcg tgctgcggaa
    29641 agtcagaaaa acagatactg gattattgaa aatattttga ataattctta tgccgatttt
    29701 cgtcgtggta attttcatta tcatctgaat ggaatggatc ttttttcttc cgatgcggtt
    29761 aaagcccgcg ctggtgttac cgaaggtgta acacttgttc agaaggcata tcgccaaaaa
    29821 cctggacttt ttattatcag tttattcatg ttggcaaaat ctgatgagct ggtgagtatt
    29881 tttactcctg ctccgatggt ggagaaaaca aaagtggtca atattctcaa aagcatcgat
    29941 ccggccaatt catccaaata caatcaggtg ctgacaacaa atccataaat atggaagcac
    30001 attttgatcc tgaatttgaa aaggaactcc gctattatat cgatctggat ctggcctcaa
    30061 atgccatctg tggctgggga tatgatttgc gtgagaagtt ggtgaatgta aaactgaaag
    30121 caattcaaca ccgtattttc atcaccaaag gtcagtacaa caagcttgtt cagaaagcat
    30181 ccggcataag gaagaaataa tcagttctgc gtagtcttaa aataaaaaac tgttggacta
    30241 atgccagcct caattttcta atgactacgc cgaactgagt ttaacgtcgc ctttaaactt
    30301 tacgaacttg ataaacttta tgactcaaag cgcacagcgc tatatcctgg ttacatcaat
    30361 ttcaaattca atcggatgtt tgctgccaca atgcaacatg cactgatcac acacgctaag
    30421 ttcgcctttc aggcgtcgtg acaccatttc agcggtttga tggtagagag attctaccgt
    30481 tattttcctt acaacttcat cagcaaatgc gtacatgagc aacatctttg cattgcgttc
    30541 gcagatgcct cgttgcttca gataaaacat cgcattgtca tccaactggc ctacagtggc
    30601 tccatgtgag cattttacat cgtcagcata aatttcgaga aatggttttg ttgaaatctg
    30661 tgcagtatct gtcagcaaaa tatttttatt gctttgaaac gcattggttt tttgtgcatc
    30721 tggcttgaca atgatatggc cattgaatgc gactgatgct tcatcatcga gtatgccttt
    30781 gaataattcg tgcgaagtgc aattgggaac gttgtgacga accaaaacct gattatcgac
    30841 gtgctgtttg cgatcgacca gatacagccc cataatttcc gcttctgcgc cttcaccgtt
    30901 aaggtttaca attgattcgt tgcgaatgtt gccgccattg aatgtgatgg tattggtcga
    30961 aagctttgca tcgcgctgaa gaccaaaaaa agaagtattg gtcattacgg cactgtcatc
    31021 cttattttgt agtttgtaat ggtcaagatg ggatccttca gccaaatgaa actcgctcac
    31081 aatattcaag aaactgtttt tatcttttat gctgtcgtcg cattgcagaa actgcagttt
    31141 gctgtttttc ccaagcacaa tcaggttgcg gatttgcaga aatggattta cttcggtatt
    31201 gacaagattt accagttgta caggtttgtc aaaaacaata ttgtcgggaa tataaataaa
    31261 gaatccggcg gtggcaaggg ccgaattgag acttacaagg ccattaacgg agttctctgt
    31321 gagcttgctc agatattgtc cgaccaactg tggaaattca tgccatgcct gacgcagact
    31381 tcctgcaata acaccatttt ccattactgt cagcggggcg gtgttgtaaa cataagcacc
    31441 gtttaacagc gtgaataatg ctgtttcaaa attttcaatc gagcaatgaa atacctgatt
    31501 gatgttcaga tttgatacgg gatcggtatt ttcaatgtat gtgtattttg cattcaggta
    31561 ttcactcaaa tctgtactac gccatttttc catttttttg tgcggaaatc cagcagataa
    31621 aaatgcatcg aatgctttgt ttcgtccatc ccacaatccg tcgttctcac tgattttttc
    31681 agcagctttt atcaatgatt ccgcaaccgg atatttttgt aattcagtca tgatgatgcg
    31741 gtttatgcaa attccttttt aatccagtca tagccctgtt tttcaagttc catggccaat
    31801 gttttgtcgc ctgattttac tatacgccct ccgtataaaa tatgcacaaa gtccggaacg
    31861 ataaagtcca gaagtcgttg atagtgcgta attacaactg ttgcgttttc cggttttttc
    31921 aaacgtgtta cacctgttgc aacaatgcgc aatgcgtcaa tatcaagtcc tgaatcggtt
    31981 tcatcaagta tggccagttt tggttcgagc atggccatct gaaaaatctc atttcttttt
    32041 ttctcgccac ccgagaatcc atcattcaca ctgcgtttca tgaggtcggc cggaatttca
    32101 actactttct ttttttcttc taccaacttc agaaaatcgc cggcactcat aggttcctgt
    32161 cccaaaaatt ttcgtttctc attgatggct gttttcagaa aattcataat tgatactcct
    32221 ggaatttcaa cggggtattg aaaacccaga aaaatacctg ctcttgctcg gtcttcaacg
    32281 gccaggtcaa agatgttttt cccttcaaaa gtaatttctc ccgaaatttc atacccttcg
    32341 cggccggcaa taacattggc cagcgtgctc tttccggttc cattgggacc catgattgcg
    32401 tgcacttcgc cgggtttgac atccagattg acgcccttca gaatctcgcg tccattgatg
    32461 gaaacatgta agtcttttat gtgtaacatt gttgtcttgt tgtttttcgt tgctatttct
    32521 gaccagtgat cggtgttccg gaattttgca aattccggaa agtgaccagt gacctttatt
    32581 tgttgtattt tactttcatg aaaatccgca ccaattcttc agcttcattt aaaaacggtt
    32641 caacttcttt ttttgagaat ctaccggtgt cattaagtat ctcaagccaa tatgctgatt
    32701 catcaacctc ttctataaca acatccagtt tatatttgta atctctttct gatttcgacc
    32761 tgcataatgc tctgaagttg gctccaactg atgatgaaca gcgtaacaat tgttttttaa
    32821 ctatcccgta aaaaacatca ggcctgtctt cttccagaaa gttcacaatg tcgatgttga
    32881 acttctttgt tctttgcctg aaagtctcat taaatgttcc agccatattt attttttttg
    32941 ttcaatgttc tttgctcact tctcaccgct cattactcac tgttcaccat tcactactca
    33001 ctgctcaccg ctaaccaacg gacccctcga gagagatcgc cagcaatttc tgagcttcga
    33061 ccgcaaattc catgggcagt ttattcaata cttcttttgc atagccattt acaatcagtc
    33121 cgatggccga ttcactgtca agaccgcgct gctggcagta aaacagctgg tcgtccgaaa
    33181 tttttgacgt ggtggcttcg tgttcaacaa tggagctttt gttgtgaaca ttgatgtatg
    33241 gccaggtgtg cgctccgcat ttgtcgccaa ggagcaaact gtcgcactgt gtaaaattgc
    33301 gcgaattaat ggcttttttc atcactttca ctagcccgcg atagctgttc tggctttggc
    33361 ctgcagagat tcctttcgaa ataatggtgc tttttgtgtt ttttccgaga tgaatcattt
    33421 tgctgccggt atcggcctgt tgaaaattgt tggtaacagc gacggaataa aattcggcta
    33481 cactattatc acccatcaga atgcagcttg ggtatttcca ggttatggca gagccggttt
    33541 caacctgtgt ccatgagatt ttgctattaa cgcctttgca caaacctcgc ttggtgacaa
    33601 aattataaat gcctcctttt ccttctttgt ttccgggata ccagttttga actgtgctgt
    33661 attttacttc agcgttgtcg taggtaataa tttcaacaac ggcggcatgc agctgatttt
    33721 catcgcgcat aggagccgtg catccttcaa gataactcac gtaagaccct tcgtcggcta
    33781 caataagcgt gcgttcaaat tgacccgttt cacgtgcatt gatgcggaaa taggtggata
    33841 attcaactgg gcagcgaacg ccttttggta tgtaaacaaa cgatccgtcg ctgaaaacag
    33901 cggaattcaa cgctgagaaa taattatcgg taacgggaac cgacattccg agatattttt
    33961 ttacaagatc gggatgttga tgtactgctt cgctgatggg catgaatatc accccaagct
    34021 ctttcagttt atcctgcatg gtcgttttaa ccgacacact gtcgaaaacc gcatcaacag
    34081 ccactcccgc aagagccatc tgctcttcga gcggaatgcc gagtttgttg aatgtgtcaa
    34141 tgagctcagg atctacttca tccatgcttt ttttttctgc tttttttggc gctgcatagt
    34201 agctgatatt ctgaaaatcg atgggtggaa tattaaggtg tgcccattcc ggaatcgaca
    34261 tttctttcca tttgcgaaag gccgccaaac gaaattcgag catgaattcg ggttcatttt
    34321 ttttctttga aataatccgc actacgtctt cattcaaccc cttgggaatg gtgtccatct
    34381 caatgtgcgt ttcaaagcca tatttgtatt cacctccggt cacttcgttc agtgcgttct
    34441 ggctttcatc ttctttcata tacgcgtttt tattcaatca ggtcacaaaa tagccgtgac
    34501 ttgttttcac ctgcaaatat acaaaatgca cgcatgccca aaccggatta tcggtaccgg
    34561 attagtgata attgtcagga cggtcaagtt ttgccacgaa tgcagacgtc ggggaatcag
    34621 ggtgtcatcc cgagtgtccg ccagctggcg gatgtatcga gggattacac cacactcacc
    34681 ttaatggtat ttgctcttcc tctttgctct atgggcatac tggctgtgtg aataatagtg
    34741 tcgccttgac atacaaaatt gtttgatttc agaatttcaa tagtgtcaca aatactttga
    34801 tcggtacttt ggaagttgtc gtagtaaaat ccgcgcaggc cccatgcgag cataagtgtc
    34861 cgcaataatt tgcggtcgtt gctgaaaacg aatatgtggc ttcgtggtct gaatgatgac
    34921 atgcgcaccg ctgaagatcc ggttgcagtc ataactacaa tggcttttgc gttcataaat
    34981 tttgccatac gcgaagccat gaaactgatt tcgtatggag tggaaacttc atcttcgctg
    35041 cgtggcatgg cgcctttggt ataaggcctt gaatctcgtt ccacgtcaat gataattctg
    35101 aacatggttc caacggtttc aacaggatat tgacctacac ttgtttcgcc gctgagcatc
    35161 acagccgatg cgccgtcggt gacggcattg gcaacatcat tcacttcggc tcgactgggc
    35221 atagtgctcg tcatcatgct ttccatcatc tgtgtggcaa caatgaccgg ctttgcagca
    35281 gtgacggttt tttgaataat gtttttctgc aaaatgggta cttcctgcaa ggggagttca
    35341 attcccaggt caccgcgtgc aatcataata gcatctgccg cttcaataat ttcatcaatt
    35401 tgctcaatgg cttctggttt ttcaattttg gcaacaacac gggctgtttt gcccgagtct
    35461 gcaattcgtt tttttaattc tataacatct tctgctttgc ggacaaatga aatggcaacc
    35521 caatcgacat cggcgttgag cgcaaattcc aggtctttta aatctttttc ggtcaatgcg
    35581 ggcagactca cattggttcc cgggaggttg aatcctttat ttgatttcag tattcccgcg
    35641 cgaatcactt ttgcctcgac ttctttttca tttaataccc gtgtgatatg cacttccatt
    35701 ttaccatcgt cgagcagaat tttttcgccg ggtttcagat cgcgcgaaag catgggataa
    35761 cgcagcgtga aacgatccga agtgccttca cattcttcgc tggtaataat aaccagggca
    35821 tcgtttttca attcagcgcc gttgcagccc atgtcgccga cacgtaattt gggaccctgt
    35881 agatcggcca gcagcgctac cgggaagaga atatccgcat ttattttttt tatttcttca
    35941 acaatttctg ttgctttggc ataatcaata tgcgagaaat tgaggcggca aacattcatt
    36001 cctgcattga ccaatgcgcg caacatttcg ggggaagagc ttgcaggtcc gatggttgct
    36061 ataattttgg tgtggttgag cgttgtttct gacatgaatt tttttatttg taaagctaca
    36121 aaaaaatgcc gcgcctttcc gtggaaagac acgacattaa agttttatta atagtgcgct
    36181 tatttttcga taatgaaatc atcaatcttc caggcgctgt agttgccgga agtgtttcct
    36241 gaactacgat aatggaaagc gatgtaaatg gtttgtcccg aatatgaagt cagactgata
    36301 tcgccgcttg gtgtgtatgt tgcgctgcct gtaggcgacc aggtggctga gagcgttgtc
    36361 catgtggcag atgtcgggtc gccgcttcct gaataatcgg ttgaaatgag cacctcgatt
    36421 ggtgtagcca tgccggcatc ggtgtaattg gtccatgaag agaaactcag cacgggattg
    36481 gcaacgccac tcaaattgat agcaggagta atcaggtagt catcacaggc aacatcgctg
    36541 ctgctggcag taacggccat atctttgtaa gcagaattga agtaccagtc tttgtttcct
    36601 gcagcactga aaatagtcca tcctgagtta accaatggat caatattgaa aggatcgtaa
    36661 ataacactgt ttgcatttgg gtcataccag ttgacaatgt cgttcaggtc gcggatatag
    36721 agttgataat cactgttgaa aatactcaga atgccacgga tgctgccggt tccgtcggga
    36781 atgatatcag ctgcaaagct ggcgtaattg ctggtgcgga caagaatctg atttccgttt
    36841 gcatcttcca gtgtgcggtt ggtggtacct cctgtttcgg aaaaagccag tccgggttca
    36901 gtgaaatgaa cattgtcaat ctgaatcagg gtgctgagat ttgccggagt aatggcaggc
    36961 attgtaacaa ctgttgctgc aggcggattt cccggtaaac tgtcgcggta aacatgactg
    37021 tcaaccaaaa tatccggaat gcggccaatg gcatcattgt aaatgtatcc aatctgaacc
    37081 agtccgccgt aatcgccgag atacagtccc tgacattcaa caaagacttt ctgtccgacc
    37141 ttgaaagatg tgtacatatc gtatctgtca agtttcagtt caatgccgcc ggtggcgtcc
    37201 tgaataacaa tggttttgta aaagttgccg ctttcgtcgt tggcaacaac aattccttca
    37261 atgattgtcg aagtctcaat gctgtcgagg gttccgggat aggatgcctt gaggtcggca
    37321 atggtggtgt tgctggtccc ttcatattcc ggaataatga catcgggtat gtcgaagtct
    37381 tcgttcacgc acgaagtgaa gaccacagcc atcagggctg taacaaaaaa caattttttc
    37441 atatactgtt tcattttttt tcgctttaaa ctgttagaaa ttgaaagaca ccatggccag
    37501 gaagtttctt ccatatgcat agtaatattt gggtgggaat tttccgacat tctgtgtatc
    37561 gaagtcgaaa cgtgactgct cgtatccgcc ggtaatcagt tccctgttgt tgagaatatt
    37621 gctgaccata aaattgacgg ccagtgtttt accttttatt ctccaggatt ttccgattga
    37681 agcgtcaagt gtatatccgg atggtaattt ttcctgagct gtgatttcat caatcatggg
    37741 gtcgccggga ccaaggtttg caatggcaag ttgtgtgcgg cgttcgggat taaaatcgag
    37801 ataaatcttg tcgaaataat tgaaatttac attgaaaaac cagtatttcg gatgattgta
    37861 tttaagcccg attgatgatg caatctgtgg tgttcccggt acataaaaat atttgcagta
    37921 aataagttcc gttgtgtcgg gtttcgaaag gttgtcaaat gaaatgtgcg ctgtcggacg
    37981 cgagatatac cggtagttcc cgaagtttcc tgcggcctgt acactgatgg ttttgctggc
    38041 cttgacgtca acgccgaatt caacaccctg atatacgcgg ttgatgccgg tcaggttcat
    38101 gtaaacatag gtctgatatt cgtcgtgata aaaactcatc agatccactt cgttgaaagt
    38161 gttggtctga aaagctgtta cacgtcctgt aacaaattca ccttttttaa tgtatgaaat
    38221 atcgccggaa aaaatcacgc tgctttgcag atttggtaca aatcggttgc tgatatttgg
    38281 cgaaaggaat gaattcatgg ttgaaggggc attgctgatc agggctgtgt tgacggtgaa
    38341 ataatgtttg cccgagtact tataggtaac gcctgcttta acaccgccat caataaaggt
    38401 ctttatttca gattttccaa aagagttttc gggataacga ccgttgcggt aaataccatc
    38461 gcgatgcatt tgtgtgccgc ttataaagcc ggctacgtag aagtctgtct tggcataata
    38521 aaagcgactc attccccaca atttggcagt ttttgtgtgt aatttgtaat tgtaaccaaa
    38581 ttcatcaccg acattgataa ttctgtcagg attgttcagg tcgttctgaa gcattcctgt
    38641 atcacccgga aaatcacgtt cggcaaactg atcgatatct ttccagaatt cgcctccaag
    38701 cagatcgttg agttctttga aataatgggt tgtttgtgag ttcacttcaa gcccggtact
    38761 gatctgaata ttctcattca ttttgtaaag cagatacgaa ttgagggctt tgcgggtttc
    38821 gtcttttctg gcttcttcta taatataatt ggcttgtgta ccagctgcat ttgcgctgta
    38881 attgatctgg tatatttcgt cccagttgat ttgacgagct tcaacattgt tgagccagta
    38941 atcggtaacc agtccgttga tgtttgcgtc gggctgataa cttggcaggt atcggtagta
    39001 gtcggggcgc ggatctggtg cgccatacca gttaagtcgt gtagttccga aacggccttt
    39061 tgtaaaacct gcggtagtat ataactttgt tttttcgttg attttaaaag tgtgattcag
    39121 cagggcatat ggctgatgaa ctttacggac acgtgcattt cgttcttcgc cgttcagcat
    39181 tccccagttg ggattgtaat aaactgttcc cgccaggtcg taagcttctt gtgtcgatgc
    39241 ggcctgcatg gctctgcgaa aaggtgagcc gaatacagtc agaccaacca tgtgcttatc
    39301 gtttattttt cgttctaccg atccaaaata ggcatatgca tcgtaccatg ttcccggaac
    39361 ataaccgtcc agagcccatc ggtgtgaacc tgagaacgca aaggcccatc cattgttttg
    39421 cataccagtg gcatacgtgt acatcaaacg gttgttgtat gaacgattgg catatccgta
    39481 actgatctga ttttgtttgc ggatttgaga tgcagtggcc tgaatatttg atgtaccacc
    39541 aattccgcca aatgaaaatg gtccgggggt cagtccatcg tatgattcgc ggtaacgaac
    39601 aactctgttg agtccgccat agtcagaata tgactgaaag ccggtttcaa gatcattcat
    39661 ggggaatccg ttcaggtagg tctgatctga acttgattcg tacccgcggc tgcgaaagcg
    39721 catggaaccc cagctgaatg atgcgaaatt ttcaaatata tcactgcccg aatgcaataa
    39781 actgttgaca ctctggccgg aacctccctg atcaaactcg ctgtcatcaa gctgaatctc
    39841 tgaaattcct ccgatttctt ccatggtttt tcgcttaagc tgaacgctga tggttgcagc
    39901 gggcgatgca atggatactt tctcggtgtg catctgaaat aaatttgctt cgacaatcaa
    39961 tgtatatgtt ccatatggga gggtgacgga aaaattgcca acagcatctg ttttaatcag
    40021 ttggttgtta atcttcactg tagcgtccgg aaccccctcc agagtaatgt catccttaat
    40081 ggtgccgctc agtgtacctt cctgtgccat cataaacgtt ggcaacagca aaagcaataa
    40141 atagcagagt attctcatac aaaaaagttt agccgacaaa tttaatgaat aattcattgc
    40201 agctttaaaa tactgtgtta tatcatgaga aattaaaaaa caaaaatctc attaaatacg
    40261 accggtatat cattatttca aacaaacagc tttgatatca gtatgtatgt acactgtttt
    40321 gcgctgctgg taaatactgg atacccagcc aggtaatcat taaaaacaga aaggcgaaaa
    40381 tgagtatcca ggaaactact ttttgttttt ccggtgcgcg caaacggatg tgaatgtaaa
    40441 gcaaataggt cagccaggta atcaatgccc aggtttcctt tggatcccag gtccagtaat
    40501 gaccccatgc tactttggcc catatagctc ccagaatgat cccaacagtc agaaatgaaa
    40561 agccaaaata aacagtattg tcggtgtatt ccatgctgtt tttgaattgc tttccgaaaa
    40621 gtggcattac gagacggata gctgcaatca gtgccgacat cccaagcagt gcatatccaa
    40681 tgatatacac caccacatga ggaacaaacc aaacactttg caatgcgggc atcagtgtct
    40741 gatcaagcgc agcaggattc aacaggtcaa tgattatgaa tacggcagcc gaaatgtttg
    40801 cataaacaaa cagccagacg tatttccatc tgaaccagag caacaccccc aataatgaaa
    40861 gaaagaaacc ataccataag cgtgtctcac ccagcgttcg gagtggcgga cgttcaagct
    40921 ctatccatag actgacgatg aaaaaagtga gcattacaac agccactgaa gcaacagcaa
    40981 cagctgtatt acggtagcgt ttaaacagca tcagaatgat tgacagcaac agcaatccaa
    41041 ccgaaacaat tccgatatat tgaaaactga tgacggtcat tcttttttct tttttgaatt
    41101 tactccggtt aaaaacatga aaatggcacc acacagcagc aggcacaatc cggtatatat
    41161 caccggcagc catgggtcac gtaccaactc gaatatactc gaactactcc attttcccat
    41221 ggtttcatcg tatccaaact gatatatttt ccagccatct actgttagtg gccgattgac
    41281 tgctaccgca gatgtgactt ctgatccctc tttggtgtaa acaatgatat ctgattgata
    41341 taatcttgct tttggtggtt tcatgagtag ctccagatct tcattgatgg ctattgagaa
    41401 tggctgctgg agcggtgatg cagagctaat ccagccgcat ttttcatttg cattttcgga
    41461 tcggatgcaa accagagctg cctgaaccgc gcccggaata ggggcttcgc gaaatgtatc
    41521 gcccatcagc catccatttt taagaatttg tttgatctca aattcgtatg actcccattt
    41581 gaaaatggtg tcggtctgtt gcagggtgaa tgatggattt ttaagcagct tttgttgtga
    41641 ttgactccaa atgccagttt gagggggata gtattcaata gtgaaatcag tcagatagat
    41701 ggcaatgggc aattcatgtt gctttccgtt cttgtcgctg cctatccatt ctatgttgtt
    41761 tttttcaaca cgcatggtat atcgttcgag gtctcctgac ccgaaagcgg ctcctgacaa
    41821 aacaatccat aaccccagat gatttagcat gaatcctgcg tttcggggct tcagtgaaaa
    41881 acgtcgaatg atggttagtc ccaatgtagt gagcaaaaat aactgtgnnn nnnnnnnnnn
    41941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42001 nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tcaggataag ggaccccatc ttttacgccg
    42061 agcgacagca ggattttttg tagttgcatt ctcgcctcac gtgctttgtc aagtccatga
    42121 ttgataatac gggacacttc gaggggcgaa tggaatgagc tcccatggct ggctgcgaca
    42181 taatcccatc gccattgtgc ctgacgtata agtttcaaaa caggcttcat ttgctcctct
    42241 gtagctcctt tgtcccatgc ggctttggct tcgaaatgcg cccatacaat ctgaatttcc
    42301 agctggtcgc gtatttcttt tattttgtcc tgtcgtgaat aaacgtcgtc aatcagatct
    42361 tcagtttctt cgcggtgaca aaccatgcat gatgaagaaa tattgttgag cgggctttga
    42421 atgtgatggt cagaaaattt ttggccgcct tcgtttttgt atggcatgtg gcaatcggcg
    42481 catgaaacac ctcttttgta atggactccg gtcataaaaa gctcgtaatc gggatgctgt
    42541 gccttaatca taggagtttt cgaaatgcca tgtacccagt ctgtaaattc actgttatcg
    42601 tagtattttt caatctgttc aactgtaaat cccgaatccc aggggaaagt cagataattg
    42661 gctccttcgt attttttctt gtcaaaataa tactcaacgt gacattgggc gcaaacaagt
    42721 gttctcagct cctgatgggt cgatttgttt acgtcttgtc ctctgcgttc aaatgcttca
    42781 ataagcccgg ggcgggtaat ggtaagattc atggtcttgg ggtcgtggca atcgagacat
    42841 ccaattggat ttacaatttc agcacccaat gattcccagg ttccggtgta aaactctttc
    42901 actccttttt ctttcattac ccgtacaaca tctgggcttt tacaagtcca gcaagtcgat
    42961 ggctgaggtc cgggaacacc ttctttgggt gcgcctgtac gcagggtgtt gtgtacatca
    43021 tcaatggcat gaaaatgacc tcgcgcctgt ttaaaatcct gtgaaaagga atatcccgca
    43081 aacaaaatga ccagtcgggg atcttcagca agaatatctc tggtggccga acctccgtgc
    43141 ttgcttttga aacttgtgtc cgccgttttg agccaactgt cgtattccct agggaaattc
    43201 cggccccaga tttcgcttct tggttcagtt tctccaaagg ttacatcggg cttgtaggtg
    43261 aattcggctt cggcgcgccg ctcaataatc gatgcagcca gcaatccaag gagaaataca
    43321 ataagtactg ttcccagaaa aagaacccag cccagccagg gcttttttga taccatttcg
    43381 gtgatggatt tcatatctta tttgttttta ttcatcattt gatcaagcca gcccggaacg
    43441 gccgaacctg gctttggaac acgtgcattc gggacactgt tgagactatt gacacgtccg
    43501 tgaggtactt cgcggtggca ttcccagcaa tgtcgctcac ttctgaaatc atcataagag
    43561 ctggtgtaca tttttgcgtt tccgctgtga agcagatccc cgtggcagcg tatgcagttt
    43621 tgctgaacca ctctttcgcc ggcttctttg atgaaaatta cctgtggctc agccctgagg
    43681 gtaaagatgg tcgcatggcg catgccatcc tttgctttga aatagtagtg attgaagaaa
    43741 ttgtcgtgtg gaacatggca gtcattacat acagccctgt cgcgatgaga gctgtgaaac
    43801 catgtgctgt attgcggtgc cataatatga cagttcatgc aggtcgccgg atcgtcgttc
    43861 agatatgtat gcgctttgga aacataaaac agaaatgcac tcagaccgag aaatattccc
    43921 gttagaaata tcaatggaac tctccatttt ggtggcggca gcaatttctt aacgaactta
    43981 atcatgaaat taagtaattg aatatcgaaa ttcttgatgg caaatataca atcagattat
    44041 aaatttgcca actatttttg caaattggcc gaataaataa tcgatttctt tgacaatgaa
    44101 tgttttgcaa aaaattgttt tatgagtatc tgattcaggc atacattttg aaaagtgaat
    44161 gagctattga atttttcacg atatttgcaa cccaatttac tgttatgaaa actattctgt
    44221 tgttatttgt tttaattgct ttcttttttg gtgctcaggc acaggagaaa tcatataacg
    44281 ttacctgcgt tgctttctat aacctggaaa atctgtttga caccattaat gatcccaata
    44341 aaaatgacga ggaatttctt cctgaaggat cgaatcggtg gaccagtcag aaatatgccg
    44401 aaaaactcag caatatggct acggttatta cacagattgg tgatgaatat atcaaaggcg
    44461 gccctgtctg tcttggagtt tcagaggttg aaaatatcgt tgctctgacc gatctggcca
    44521 atgaaccaaa aatgaaagca tcgggttatg tgccgttatt gattgaagga cctgaccgcc
    44581 gcggagttga tgtggggttt ttgtatcgta aagatttttt cacaatgatc agtgcgacct
    44641 cgagccggct ggttgttgca gatcagcccg attttaaaac ccgcgatcag ttgctggtga
    44701 aagggatttt gctgggcgat accattcata ttatggtcaa tcactggcct tcgcgttatg
    44761 gtggtgaaaa acgaagcgct ccgatgcgcg ctgctgctgc caaactgact cgttcaacaa
    44821 cagattccat tatgaatgtg aatcccaatg ccaaaataat tattatgggt gacctcaatg
    44881 acaatcctac caacgccagt gtttttaaaa tattaaacgc ttccggcgat aaaaaaacgg
    44941 cgatggatat taccggatta ttcaacccaa tgtacaacat gtttaaagtg cagggaattg
    45001 gctccaacgc ttatcgcgac agctggagtc tgttcgatca gatcattgtt tcaaaaggtt
    45061 tgcttggcga tgataagtct tcactgaaaa tgttcaaaac actcattttt aaccgcaatt
    45121 tcctgaaaca ggttgagggc tcttttgccg gatatccgta ccgcactttt gttggtggtc
    45181 agtatcaggg cggctattcc gaccacttcc cggcctatat gtttctgatt aaagaaaagt
    45241 aaatgaacac tgaactctga acaaggaatg acgaaggatg aagtgaatac cgaacatcaa
    45301 atcattaatc tgtctgccag attaactgca acaggcagtc aggcagacaa ggaataccga
    45361 attcagaagc gaaatctgaa tcaagaaaat gcttgtttga gtgttcagga aaggtctctg
    45421 atcgctgctc accgctcact gctggcgctt ccgacttatt cttaaaagca actttaaaac
    45481 tttcaacttt acaaactttc aaacctttcg ctaccatgac cgctctcgaa acccttctcc
    45541 aacaccgcag catacgcaaa tacaaacagg atccggttcc ggaagatatt ttgaattcga
    45601 ttctcgaagc cggctcacgt gccgctacca ccggcaacat gcaggtgtac agcatcgtgg
    45661 tgacccgcga tcaggaaata aaaaatcaat tgctgccatt gcatttcgga cagaaaatga
    45721 tagccgaagc tcctgtggtg ctgacattct gtgccgattt caaccgtttc aatcagtggt
    45781 gcaatcagcg taatgcggag ccgggctacg ataatttcct ttcgttcatg aacgcagcca
    45841 tagatgcatt gctggtggcg cagaattgtg tggtggccgc tgaacatttc ggactcggca
    45901 ctgtttatct cggaacaaca acctacatgg ccgataaaat catcgatgtg ctgaaacttc
    45961 cgaaaggtgt tgtacccatc actacagtga ctatcggtta cccggctgaa ataccgggcc
    46021 ttacagatcg gctccccatg cgggccgtag tgcattatga gacctacaac gaatttgatg
    46081 cagccagcat cgacgcgctc tatgcggaaa aagaaaattc cgatgaatac aaaaaatttg
    46141 tgaccgacag ccaggttgat aatctcgctc aggtattcac gcagaagcgc tattcaaaaa
    46201 aagacaatgt agctttctca aaatcgctgc tggctgtttt gcagaagcag ggttttatga
    46261 ataatgatta atcatgacct caaatctttc aacagcatta ttgatttcag gcggcgtcga
    46321 tagttcggtg gctgtgcaga tgctgatgga gcagggcatt aagcccgatt tgtattatat
    46381 tgccatcggt cccgatcagg attttaaagg attcgactgc aaaatggagg aggacattga
    46441 tttgtgcaaa ctcgtggcgc gcagattcgg tcttgctttc gacattgttc cattgcataa
    46501 ggaatattgg gacatggtgg tgcaatatca catcgaaacg ctgaaagccg gacgcactcc
    46561 caatcccgac ctcttgtgca atcagctgat taaattcggt gcgttcgaag agaaagtagg
    46621 atataaatat gatcgtctcg caaccggtca ctacgcctgg attcaggata ccgatgccgg
    46681 aagattttta cacacggcaa aagacaaaca caaggatcag acttattttc tggcgcaact
    46741 cagtgcaaaa cagatttcgc gtctgatgtt tccgatcggc caactcacaa aacccgaagt
    46801 gcgtaaaatc gcatcagaac tcgattttcc gaatgccaca cgaaaagaca gtcagggaat
    46861 ctgtttcatt ggacgcagcc attacaagga ttttgtggag catttgttgg ggaaaaaaaa
    46921 aggtgatttt attgaattct caaccggaaa aaaacttgga gagcacgacg gacactggta
    46981 tttcacggta ggtcagcgca agggtctcgg tctggcgggc ggcccttggt ttgtggtaaa
    47041 aaaagatgcg gaaacaaaca atgtgtatat cgcctgcggc tacgagcccg acgaagtcta
    47101 tggccgcgac atttatatca accagccgca ttttcttttt ccatttgaat taaatgaaaa
    47161 cgaagaagcc gaagtaaccg tgaaaatccg ccacacgcct acgttttcga aagccattat
    47221 ccgcaaaacc acccaaggtt ttcacatcat caccgacgaa ttacaacacg gaattgcacc
//
LOCUS       DAOPKE010000013        44844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_867_length_44844_cov_12.772109, whole genome shotgun sequence.
ACCESSION   DAOPKE010000013 DAOPKE010000000
VERSION     DAOPKE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_44844_cov_12.772109"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKY63_03150"
     CDS             <1..192
                     /locus_tag="PKY63_03150"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPE99643.1"
                     /translation="ASMYEQLLPHTSKLYLTCVDGEYDTDTFFPELNEKEWELVKESD
                     IMADSKSGIQYQYLIFVRI"
     gene            269..1105
                     /locus_tag="PKY63_03155"
     CDS             269..1105
                     /locus_tag="PKY63_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99644.1"
                     /translation="MKQSFFFLLALTLSALTILSSCKNDEDNEDKNNYRSAIDNATAE
                     TMFSDVFKQATDGMIAAQDSAEGNKNCMESMASCATLTITPFDLTTFPKTLTVDFGTV
                     NCMGTDGRYRRGVVTMTTTGWYRDSGTVIIVTPQNYFVNDHLVQGTKTITNMGHNLAG
                     NLVYSLFIDGTVTNSDGTIEWTSTREHEWIEGEPTVFIPWDDVYLITGTAEGVNINDE
                     AFDVVVNTALRVQVGCRWITAGSLTLTSGNFTMLVNYGDGACDAAATVTINGAVYNIT
                     MS"
     gene            complement(1183..4434)
                     /locus_tag="PKY63_03160"
     CDS             complement(1183..4434)
                     /locus_tag="PKY63_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE99645.1"
                     /translation="MHQHMSSFTVLNASAGSGKTQRITLEMLRLILPNPSEASRILSI
                     TFTNKAAGEMKDRLISSLSDIARTPEKSRMLPALQQAFPQKTTAELSQMADRALKYVL
                     HHYSDIAISTIDSFMQRIIRTFARELQLPANYEVTVGDEELNELIISNIIDNANLNEN
                     ITRVLKEIIRMQMDDDNSPLRLADELLPLVQHLTQEASVNTVDSALEAGSEKMIKAFD
                     SLLSKNKNIASEIRKKLDEINSILSKENIGEKDFIKKGPSKTIFEYIMSLNASFNTKE
                     LSKTMMKILDSGQFLVNPRPEIESRIQTLLYEIIELISEYKYGRMMAKRFPVVALLSE
                     IWKFREEYKSEKNILPVSDFNRIIREVLSKEPVPFIYLRAGSRYKTIMIDEFQDTSLM
                     QWLNLLPLIHESLARGNSSWVVGDPKQSIYRWRNGKVEIMLGLPEIFEGSDDTLEAES
                     IIKSAFQKEDMEYNYRSDINIVDFNSQFYEFTKNRFIASLSEEDVSEPIKKKLDIYSS
                     VYDQIKQKPNAAAAGFIECRIYKGKLNDHKKQWLEDIVEIIRKCTDLKYNLSDIAILT
                     RQNNIGIAISSYLMSLKEAIPVISRETLQFCTSPHSRLAMCMYRLLHCPGDDLNATET
                     WLLLQQYANIDFEDDKEINNFLIQKKNVRGIMLLNYLTQKLDALNPESLVFMPPADQL
                     DIIIHTLGISDTGGFFLLFLREAIMKQQDKTGYNADLLWQWWTEKGKTTSVLVPDASD
                     AVNIMTIHSSKGLQWPIVIMPYFNIDETKSINRNLFWHETNEKQWGIPYSVVTYTKDS
                     PGKDIREQYQIEKTKSLMDTINLMYVGTTRPEEKLFLFSFLTPDKTTKESNKSKKKEP
                     TNKTTTQTNSANFLQDFIRCTNEKFSETTHENHTSWTLGNFAGKREPKEKKGIHFNVN
                     TFRVNPNKPLDSLRKSKTGFDSAELRESAMKGKIIHELLSGITEASDITHVIDNYTRN
                     GWIENELKEKYIQLVNVIVEKFAFAFPGRKFVMSEREIADKDGIVWRPDRMIFDHENK
                     WKVIDFKTGREKKEHREQVQHYSTLLTDAGMMVESSYIIYIDTENFEARAVEV"
     gene            4519..6363
                     /locus_tag="PKY63_03165"
     CDS             4519..6363
                     /locus_tag="PKY63_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99646.1"
                     /translation="MITFLMRSCLIVLIFIISSVFVFAQSLNDSLLLHYPMNGNCHDV
                     SGNNFHAALINATLTSDHNGNTNSAYHFDGATEYINWPSSLVLHPGLPITIAFWVKYD
                     SNDYLNSFLFDTDFAQDNYSGIHCNLTPSLQITFSFGDCSGSTSPAARRSKTGSSSLQ
                     INTWYFVVAVMRGATDMDIYINCVNDGGTYSGSGGSIAYTGTPGILGRSDGDMYAPAR
                     YFMGSLDEFRYWHRALSAAEILSLCNSNNPVIEASGCYDGVYNFSLQNAGSYSGVTWD
                     FGDPASGAANSSVLDNPTHVFSAPGTYQVTAVVSYYIGSPVSTNYTVVVQAEPDLIPY
                     DSVLECAGSSRVIEVEGIWDSYQWSNGAVTQSIVVSASGVYSVSATDSNGCIVADTVV
                     AVFVNTTVFETDTTFCNGSSIVFDGQVISAAGDYSFTHPSGSGCDTIINLHATVENAP
                     AVSLPGDTTLCTGTQFPISPLVSGSWDAGAWSDGVTTTGRIINIGGVYFYSATGMCGT
                     AEDEIIVAFEDCVSGIIFPNVFTPNGDIYNPVFRPDGFNIEDFHIIVFNRWGGQVYEG
                     FSISDGWDGTFKGQECPEGVYFWIAEYKVYQNNVMVADSAKGSVTLLR"
     gene            6441..7916
                     /locus_tag="PKY63_03170"
     CDS             6441..7916
                     /locus_tag="PKY63_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPE99647.1"
                     /translation="MAQGISKSFIKSSFIYSFIGALPLASSVLLLPFYGNSDLLSTSD
                     FGLLAIYIALSELVRILFIYSADNYLGINFIHNSDSPAQTRKFIGTSALFLIIFGVGM
                     TLLFSVAGDYLFTFIFPGKDVNFYPYGLLSILTGLFTGIFRAYTSLMIYRQKPNPYFW
                     SNMLHFILVIAFSVSGLYLFPMSLEGPIWGRFISAVSTFIWAMVYFAKESHFEYSKSV
                     LKDLLRYCTPLYIFYILTWVISNIDRYFILGIMTEKEVAIFDFAVKITLIVELLQAGL
                     SAAINPKVFKIWKENGDRPVGNVEINRYFNGFTVINQLSIPLIYVAVVFLVPFVVSNT
                     DLYFSFNLLPVLLAGMATRVWLMYLMTPIYYFKKTKMLPLIYGLVAVIQVLVTYILIR
                     FNGIEGAAWANFLTKMVQVFLVFIFVRRFYTFSVNPQKFIVYPAIYILILLATDLFFS
                     DINVYLMASLHLVILSVVGFLMFKKEIVPFVKSLYQGRVKS"
     gene            complement(7977..8840)
                     /locus_tag="PKY63_03175"
     CDS             complement(7977..8840)
                     /locus_tag="PKY63_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPE99648.1"
                     /translation="MRQLKISKQVTNRETASLDKYLQEIGREELITANEEVVLAQRIK
                     QGDMAALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGTINKINKAFAQLEQEFEREPDAHEIAA
                     LLDMPDTEVKDSLRHTGRHVSMDAPIVTDEDTNLYDLYANEESERPDKGLQFESLNIE
                     IERAIATLTPRERDVLKFYFGLNGAAQLTLDEIGDKFGLTRERVRQIKEKAIRRLKHG
                     SRSRNLKGYLG"
     gene            complement(8911..11037)
                     /locus_tag="PKY63_03180"
     CDS             complement(8911..11037)
                     /locus_tag="PKY63_03180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPE99649.1"
                     /translation="MNAITQTFELPDGRTGTIETGKLARQADGSVIVRIGDTMLLATV
                     VSNKEAKENVDFMPLTVEYQEKYASTGRFPGGFFKREARPSEYEILIARLVDRALRPL
                     FPDNYHAETQVLIYLISGDTDTHPDAYAALAASSALAVSNIPFNGPISEARVARINGE
                     FVINPSVKDLENADLELMVAANAKDINMVEGEMKEVSEEVMMEALKFAHEAIKKQIAA
                     QIELAAKVEKANPKREYCHEVNDEELRKAVYDFCYPKAYEVAKRCTPNKHQRQDDFDA
                     IGEEFLATIPEEELDAKKSLVARYYHDVMKKAVRDMYLAERIRLDGRKMDEIRPIWCE
                     VSPLPTPHGSAIFTRGETQSLTTVTLGTKLDDQIIDGAVFEGTNKLLLHYNFPPFSTG
                     EAKMLRGTSRREIGHGNLALRALKNMIPQDDSNPYTIRIVSDILESNGSSSMATVCAG
                     TLALMDAGVKLRKPVSGIAMGLITDSETGNYCVLSDILGDEDHLGDMDFKVTGTLDGI
                     TACQMDIKVDGLSYDILAEALAQAKRGREHILKIITATIAEPRGDYKPNVPRFVKFSI
                     PREFIGAVIGPGGKIIQEIQRQTGATILIEEVGDFGVVEIFSADKESMNAAHNWVKSI
                     VAVPEVGEVYEGTVKAIQAYGAFVEILPGKEALLHVSEIAWERIQDVNSVLKVGDKIQ
                     VKLIGTDEKTGKLRLSRKVLLPKEEK"
     gene            complement(11110..11379)
                     /gene="rpsO"
                     /locus_tag="PKY63_03185"
     CDS             complement(11110..11379)
                     /gene="rpsO"
                     /locus_tag="PKY63_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPE99650.1"
                     /translation="MNLSIETKKQLAEKYGKAVTNTGAPEVQVAIFTERIKHLTGHLK
                     SNKKDFVTERSLLALVGKRKKLLTYIKTKDIEKYRTLIQQLEIRK"
     gene            complement(11630..12235)
                     /locus_tag="PKY63_03190"
     CDS             complement(11630..12235)
                     /locus_tag="PKY63_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPE99651.1"
                     /translation="MKKLLILVIFIVAAGMLALAQPSNTGGGIEDGIVTKAVIIEGDT
                     VPLFWIPTVRIYGPIVFKSKVQVIEFTKMVRHIKRVYPYAKLIGSKVNEYNKYLNGIE
                     NDRERERELDRIEKEMRAQLESQLVKLTFTQGKILMKLIYRETNNTTYHLIRDFRGGI
                     TATFWQTFGKIFGYNLKVTYDPAGVDHDIETIVVMIENGTI"
     gene            complement(12272..13504)
                     /gene="clpX"
                     /locus_tag="PKY63_03195"
     CDS             complement(12272..13504)
                     /gene="clpX"
                     /locus_tag="PKY63_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPE99652.1"
                     /translation="MAKPTDHCSFCGRSQQEVKLLITGVSGNICDACVEQAQLIINEE
                     LALKNKKPASGAGFTLRKPKEIFNFLEQYVIGQEQAKKLLSVAVYNHFKRIRAKENDV
                     EIEKSNIVLVGETGTGKTLLAKTIARFLDVPFAIADATALTEAGYVGEDVESILARLL
                     QVADYDVKAAEHGIIFIDEIDKISRKSDNPSITRDVSGEGVQQALLKMLEGAVVNVPP
                     QGGRKHPDQKYIQVNTQNILFICGGAFDGIEKMIANRLKTSVIGFKKKGEQKEIDKEN
                     LLQYIASEDLRKFGLIPELVGRLPVLAHLHPLSEEAMLEILTKPKNSLVKQYKALFEM
                     DNVAVEFTPDGLKEIAHMAIKGKLGARGLRSIMEKIMTDLMFDLSESEKNEKIKITAT
                     FVKNKLKNHPEIQLKVAS"
     gene            complement(13511..14182)
                     /gene="clpP"
                     /locus_tag="PKY63_03200"
     CDS             complement(13511..14182)
                     /gene="clpP"
                     /locus_tag="PKY63_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPE99653.1"
                     /translation="MNDEFRKYAIGHRGISSNVFDKYSQISSSYISPTIIEERQLNIA
                     TMDVFSRLMMDRIIFLGVPIDDYVANIIQAQLLFLESVDATKDISIYLNTPGGSVYAG
                     LGIYDTMQYIAPDIATICTGMAASMGAVLLCAGTHGKRTALSHSRVMIHQPMGGAEGQ
                     ASDIEIVATEIKKLKEELYQIIANHSGQPYDKVWKDGDRDHWMIADEAKEYGMIDEVL
                     RRDKK"
     gene            complement(14193..15542)
                     /locus_tag="PKY63_03205"
     CDS             complement(14193..15542)
                     /locus_tag="PKY63_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPE99654.1"
                     /translation="MNFEFEKNGELIATLKTHIEPADYQPKVEEKLREYRRKASMPGF
                     RPGKVPFGVIKKMYGNSVLADEVLHVASEAVYKYLDDNKVNFILSPMMDEEAARLTEW
                     NEGNTFTFNFDVALEPEFEPTADKSLTFTKFEIIHDETDVEKYLGEIRERYGKFEKAE
                     SVDEKCFVSAQLIQTENGEKKEDGLDKFIHLRINTLPEHAQKFFIGKKAEDFIDFDVE
                     KDFPNADERTEIFNINIDDARAMKGTYRITLQSVLSITPAELNEQLYAQVFPERQIEN
                     EDQLREIIRNDVKGTYEKEARKQFYFDVQKELKTKYDFTLPEEFLKKFIRTRSEKELS
                     EEDLEKGLPFYIDELKWQLIENKLIKKYNLLITREDIRQHLKELLGLADMDENDPDVK
                     NKIDQVYDIISKEKERFSNLVNNMTDERIIKLFEDNCTIEAKTMNWKDFLNLVNEKN"
     gene            complement(15577..15658)
                     /locus_tag="PKY63_03210"
     tRNA            complement(15577..15658)
                     /locus_tag="PKY63_03210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15622..15624),aa:Leu,seq:tag)
     gene            complement(15863..17494)
                     /locus_tag="PKY63_03215"
     CDS             complement(15863..17494)
                     /locus_tag="PKY63_03215"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99655.1"
                     /translation="MKTITLTFIALFISLLTSAQLGPDVIYPKGDGNPTQKVYPFDGS
                     YGYSYHASIYNKENIGVTGTIEQIAYFVAAPCNDSVTVTIRLEEVNNAVFGGNNTFGI
                     VSMNGTQVFQQKVLFNNTGWNYFNLDNDFDYDSSYHLAVYISTAWGGTGPTTVPEFGC
                     ENITTATDYVALTWGQDNSFPTSMMSQLSYYRPVVLLKFDSPQPSIISGTLSDCGESV
                     LTAAEFGVNDNLIVVYNYTGTFDSPYCGQPYLSGDTLSDGSEIIYNGPTGQVIHTGIE
                     SNKTVYYKTWSYDEYLIYSATGNVDDLFSQRSIPFSTNFDGGPELPDGFSGGWENLIN
                     HGLTDQGVSAELTPAITVKHLYSPHFCGLTSNSILTFDYRIVNISGYPNTATPASEID
                     SINIKMNDPISGDYVLIHSINPANHIASSDFVSVEIPIGAFTGGMTAVYLSAYSGSGS
                     YFVDIDNFAITDPSGITDDGTTILNIYPNPAGNILNVLTDGSGFVTIYDLAGRQLMQQ
                     ESEGSEKLSIDVSALSPGLYLIQSDTHGVARFFKE"
     gene            17640..20411
                     /locus_tag="PKY63_03220"
     CDS             17640..20411
                     /locus_tag="PKY63_03220"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPE99656.1"
                     /translation="MKTIFLSVVLLVLALIGKAQTPIDEGWVEVGNAAYNSIIYDVVF
                     NDANNGFAVGSGGTFLKTTDGGDTWNAIYIGVNYTLFEIAFSSSNVGYMRGGNKDGGG
                     YFGKILKTTDGGVTWTEVLSDPSTFFETMSVRSDSVVFVGDYEQTQHTVDGGASWIIT
                     PLTGLYTIAKIGFEQSGNGFLIANNSRLYLSDDDGQTWDSVFYFPGDTPLDFSFADAA
                     DGLLMSYNRKLAISNDSGATWALHKTLTEMMIDLEFFNDSTAYFLKNYGNTIYKTQDT
                     ASTLQISYSNLQADLNGLHKMSDGSLLATGGGGLIIRTIDGIIWDTLHIGEMNTNLTD
                     VVFIDNQTGFAAGSNGYIRQTTDGGFTWITSQINAGIDLGGICAATSNRLIAAGNETS
                     CYISTDTGMTWTQHMYGVDMGLARGIMRASATQLFAFGSEGIFMTNDAGLTWAESGSY
                     SQVYSGFVLNTDSAFFGFQNAGIRYTFDGGVNFTDIITVNPAIQGIHFFDADTGLFVN
                     SWGRMYRTTDRGVNWSQVLNSGISLQELYFIDDTLGYAMGNSGRLYKTTNQGINWQLI
                     ETGTTRTLYKMWFTPDGTGFIVGDDGIILRKAATPVFDIQFTVTNDSGDTLINAAMTF
                     NSVAYPAGINEVSGLNSGSYNYIISCPGHLSDTGSVAVVSDTTIVIELKKFHTVTFQL
                     KNVFNNPVSAAGVLFNGDSLVSNSAGDAVFGNVLKSPSLPVQVNESHYLPFSSATGIA
                     NDTTISILLQADIAAPVPNTVTVIDDYSFTASWTAGTNATQYALFVSDDNFVSTLPGY
                     DSVIVSSLNYPVTGLTAGQTYYFRLRSLNSYGYSAYSSVGTAETTTGIKEISDCLIEV
                     FPNPANSFVVVNPLGVMFPQRVDIFSVSGQLVMSHEINGIEIIDISALQPGVYYLRIM
                     DYVQQIIKQ"
     gene            20422..20634
                     /locus_tag="PKY63_03225"
     CDS             20422..20634
                     /locus_tag="PKY63_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99657.1"
                     /translation="MKESSTVEEVRKKMRKVQRCIRQNKIQEAWSNLDTIDASMIEQC
                     GESEKQLISEARQILLHIMVRELKEK"
     gene            20642..22681
                     /locus_tag="PKY63_03230"
     CDS             20642..22681
                     /locus_tag="PKY63_03230"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPE99658.1"
                     /translation="MKTIILAFTLIVPLLTAAQWNWLNPQLQGDPINDIVFVSANDGF
                     AITGGGALMKTTDAGTTWTMEGCIAQGYLTDLHFGDATHGFACGSKGVFVRTVDGGNT
                     WNVINTTFNSVDFSGIWFLNNTTGFIIADYGVMIKTTDGGDTWTAVSTGSTETFTDIY
                     FPSATTGYVSTEDGYFMKTTDGGATWTELDTYQAETFKCVWFTDENTGWMAGNWGAIY
                     KTTNGGTSWTSQSVATHDYFNAICFVDASTGYLCADGTVFKTTNGGTTWTQCYNPNYD
                     YLYSIYADASGRVFAGNERGRLYRSVDAGNTWADLTTDLMTPSVRLNAVAFFNDQTGI
                     VAGSNGVLFRTENAGLAWDSIPAGTFQEIYDFELVSNTEAYASAGAGTILHTSDAGET
                     WTSIVSGTTNTLYGIKKINASFAVACGWAGTVLTSADGGSTWTSISTGFTTGLYDIDF
                     YNGSTGLCVGQSGKIYRSTDGGQTWAAITSGTSQYLNAVCFVDSLHVVAGTNTQGLLY
                     SSDGGLTWSDPATNVDMGITQLYCPSADTVFACGMDGLMAFSTDMGLNWTGMDRMTYW
                     LLNDISKTPSGKMFAVGYNSTILFRGQLQSVGFENFVEKADLSLHVYPNPAADCIYIE
                     NGHQQWYMIFDEQGRMIQSGYASENSIDIRSLAPGNYFIQCSSSESVQTGRFIKK"
     gene            22814..24541
                     /locus_tag="PKY63_03235"
     CDS             22814..24541
                     /locus_tag="PKY63_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HPE99659.1"
                     /translation="MTNLTKRIVFAVWLTIIFSLAGIGQNDAVLDSLLEQAAGERRNG
                     AYDKALNLDFTVLALAKNMGNDAMLAAANNGIATDYMRIGDLKKSRIYYEQALEIYRH
                     LRDTINIGDQYYKLGSLDVDEGKMDFAREKYSLSVAVFRKKNYYPGLADVYNGYAAIF
                     YIQQNLDSVAWYAEQSLKYYRLAGNTDAESFMYINLGALFNAQKDHPKAIQYVRRGIQ
                     LADSSGLMNQMRQGYRNLSETYAMLGDWKQAYDNQLLYVRYNDSIFNQQKNEAMVKYE
                     ALYQTSEKERKIQEQNADLLSKENRIKSIQNTRNILILLFIVALIVALFFVYRYITRK
                     KINRLLDEKNKQLEEINAFKDRIFAIISHDLRSPVTSFSRITSALNTAIDQLPPKEIK
                     KYLNDIEVSAIELQNMLRGLLHWSLTQQSGANVQTQAVLLSECIQDVVADCRGAFSEK
                     QISLETEIPDSLTVLSDKGLLHIVLRNVLSNAVKFSDTGDRIVIRVTESDGKCFIDVC
                     DNGPGVPDEVASRLFGTMASDAPSRGGGFGLFISAELLKKMNGSICLAATSADGSVFR
                     IVLPIANLV"
     gene            24543..25196
                     /locus_tag="PKY63_03240"
     CDS             24543..25196
                     /locus_tag="PKY63_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE99660.1"
                     /translation="MKTRIAIADDHTIVRDGIRLMCLTDDALELVFESATVNGFVKLM
                     EKNSFDVAVIDIQFPDGSGIDLAKTILLKFPDARLLVFSGMMDEETILKAISTGVSGY
                     VSKDASMAELRTAISTVASFEQFFSMSVGQILYKSYVKKQQQGLSETIPDAVLSDRET
                     EVLKGFAEGLSYKEIAEKLFISPRTVETHKENIMKKLGLHSLADMIRYAIKEKIITL"
     gene            complement(25209..27269)
                     /locus_tag="PKY63_03245"
     CDS             complement(25209..27269)
                     /locus_tag="PKY63_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPE99661.1"
                     /translation="MNSWSKAPFVRILIPFIAGLLIYRSGWADVAHSWILPVFLALIT
                     GLAAWFFVFKSLNTYKTRTIQGALIICCILAFGWLNAHYSDHRNKSDFIGKLPENQAA
                     ELILKIDNTVEQKNNNMKTTATSLAVKTDSGWIRCSGKILLYISNPDTTPVLSYGDII
                     AVKCYLNTVQNPLFNSDFNYKKYLADKNIFHQTYIRDDHYTILEKKQGNVIMTFALSV
                     RDKMVKILQNQLGDNDESSVVAAMLTGYRADISADMMNAYSKAGVIHIMSVSGLHVGV
                     IYIMITAFVGLFGWFKKRKWLSVLLVLSLIWFYAFLTGLSASVLRSAVMISFVVFGTA
                     LQRNISPYNSLAAAAFLLLAFNPGSLGEIGFQLSFVAVLGILLFQRPLYNLWHIQNKW
                     LDKIWQLACVSIAAQLITLPFILYYFGQFPVYFLISNLIVVPLSSLVLYAGMAAVLLS
                     WIPLVNTAVAFGTIWLTKAMNFIVITIESLPGSVMSNIYLSAWPAILLALIIALFYIM
                     FYRKKIKTLYPILALAIIASLFEWNFKTNFKKQQEAFFLKDGNEYVIGIKQDKALYLV
                     LPSDSFPMSDYLRKRLNRFAERNSLQTITAGEMFNDKMVFRHENFIGWNQLRMLNWNE
                     RKMPPAVENLPQTDLMLAPSLSKYYFEKFNGAMPAKCIMTKVRSEKPGILDLNSKKEW
                     SFKR"
     gene            complement(27276..28427)
                     /locus_tag="PKY63_03250"
     CDS             complement(27276..28427)
                     /locus_tag="PKY63_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPE99662.1"
                     /translation="MNIKILLSFCITMLYAQLSVAQQDVKVRIHADKTIHSTDIVIFS
                     GTYQLQDTAGQTVKLFNKGDSLHIAPLHYDLVILTKTDTIFCSKHFKLRGVGFLNIFQ
                     LKAGKSIRLYDDDLSISLRVDKTMWLFNEVELEHYVAGVVQSEAGIAKNSEFYKVQAV
                     AARTYTLKNITKHAADGYQMCDQTCCQVYKGRCANTEIMMATSKTAGEIIIDTLSKQP
                     IIAVFHSNSGGQTCNSEDVWGRPLPYLRSVEDPYSIGQTSYSWQKTMTRNEWLDYLQR
                     NFNYPVNDPAAVVKVTHFTQIHRQVFLTGNIGLRKIRDDLKLRSTFFNIKEDGNNIII
                     TGKGFGHGVGMSQEGAINMAKQGFSYIDILKFYYLGTEITTIDSLSKTE"
     gene            complement(28429..29538)
                     /gene="lpxB"
                     /locus_tag="PKY63_03255"
     CDS             complement(28429..29538)
                     /gene="lpxB"
                     /locus_tag="PKY63_03255"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPE99663.1"
                     /translation="MKYFVIAGEASGDLHGSNLIKHLKLNDTNAEIKAWGGDRMIQQG
                     ADVKKHIRELAFMGFVEVLINLRTIMRNFRLCKAQVSEFKPDALILIDYPGFNLRMAK
                     WAHEKKIKVIYYISPQVWAWKKSRVKIIRRTVNEMFTILPFEKEFYSNHGMNVRYEGH
                     PLIDAVEDFKNNNTQTDWNDSEKPILALLPGSRKQELKKMLPLMLEASDAFSSTHRII
                     IGAVSLLPSELYNTEGRNIEIITDQTYALLTKARCAFVTSGTATLETALFRVPLVVCY
                     KANAISYMIAKRLVGKNIQFISLVNLIAGKEVCRELIQRDLNKDNMVNEMEKLAAPGE
                     ERTRMLSEYDLLYARLGGKGASERVAKQIIKICSN"
     gene            complement(29535..29819)
                     /locus_tag="PKY63_03260"
     CDS             complement(29535..29819)
                     /locus_tag="PKY63_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99664.1"
                     /translation="MKEFFRRDNFLLGLGIAIVLPVLTWFIIQGITLIFAGSTGDLFQ
                     KSTDQLLSICANLIPFRYYLVKLKADKTGKGILLITFLMAMVYFYFNWSA"
     gene            complement(29816..30577)
                     /gene="surE"
                     /locus_tag="PKY63_03265"
     CDS             complement(29816..30577)
                     /gene="surE"
                     /locus_tag="PKY63_03265"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPE99665.1"
                     /translation="MNKTILITNDDGIDAAGIKFLISFLRPLGKLVIVAPETGMSGMG
                     HAVTIKNPLRIKHLPNDGDVERYTTTGTPADCVKLGLNQILKQKPDLIVSGINHGGNH
                     SINVLYSGTMAAAIEGAMQNIPSAGFSMCNPNPKIDFSFMESYVQEISARLMKNGLPA
                     GTCINVNFPDTDKPAGVKVCKQSIGYWNEEFDSRLDVHHQPYYWLKGDFIIPEVQEDT
                     DENALKQGFISIVPVTIDLTAHTILDITRNQFQGL"
     gene            complement(30584..31243)
                     /locus_tag="PKY63_03270"
     CDS             complement(30584..31243)
                     /locus_tag="PKY63_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99666.1"
                     /translation="MKRFLLILTAAVILFPAQAQKTKVSESIEKIGDGKNNCLVVTIT
                     GASEDDVAKAWKDKMKDTGGKVSGKKEMFVDDATLPSISPNTIDIYSVIEEKGGHVIF
                     KVAFNLGGAYLNSKEHASGYKIAEKMIYDFAVEQTKCAVQKKIDEQKDVIAKTTKDIE
                     GLTKDNEKLAKDIEDYKKRIEDAEKDIEKNKSDIVNGNQNLEDQNKALKELEKNLSEV
                     D"
     gene            31642..32163
                     /locus_tag="PKY63_03275"
     CDS             31642..32163
                     /locus_tag="PKY63_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPE99667.1"
                     /translation="MALIKKVKGILPEFGTDCVIAENATITGDVVMGDHCSVWFNAVI
                     RGDVHSIRIGNNVNIQDNVVVHCTYQKCPVSIGNNVSIAHGAIVHGCTIHDNVLIGMG
                     SIVMDDVVVESNCIIAAGAVVTKGTRIESGSVYAGMPAKKIKSIDKSLLEGEVKRISD
                     NYMMYAGWYDEGL"
     gene            complement(32640..33539)
                     /locus_tag="PKY63_03280"
     CDS             complement(32640..33539)
                     /locus_tag="PKY63_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPE99668.1"
                     /translation="MSKSIIKQQTAPEDRIFSIRGVQVMLDFHLSDLFGVETKRLNEQ
                     VKRNIKRFPENFMFQLSPDEWENLKSQNATAKPNETLWSQNATAKRRTIPYVFTEQGV
                     AMLSAILNSEVAISVSIQIINAFIKMRQLVLNNSLIEHRFNKIETKQHETDRKFEQVF
                     KALEGKKTIPNEGIFFDGQIFDAYTFVSELIKSAKQSIVLIDNYVDETVLTLLSKRKK
                     NVSAIIYSSQITKQLKLDLQKHNQQYAPIDLLVFSKSHDRFLIIDEVVYHFGASLKDL
                     GKKWFAFSKMEMTSGEILCRLRI"
     gene            complement(33502..33852)
                     /locus_tag="PKY63_03285"
     CDS             complement(33502..33852)
                     /locus_tag="PKY63_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99669.1"
                     /translation="MKNEIIIYQPNELASHIEVRVDEDTVWLSLNQIAQLFERDKSVI
                     SRHLRNVYKEGELSMEATVAKNATVQIEAGREVKREIEYLILMPSFPSGKKDILHSLK
                     FLYEQKYYQTANSA"
     gene            complement(33933..34985)
                     /locus_tag="PKY63_03290"
     CDS             complement(33933..34985)
                     /locus_tag="PKY63_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPE99670.1"
                     /translation="MFKKFKQRNHLRFRHNEAALHELSYLFWECTLRCNLNCRHCGSD
                     CSVSSQLPDMPFSDFLNAIKPLQKQYKKDSIIVIMTGGEPLLRNDLAECGMQLRQNGF
                     RWGIVSNGVLYNNNIHNKLLNAGMGSVTISLDGMAATHEWMRNSQYSYDAALNAIELI
                     SSSPRLNADVVTCVNQRNISELDEIHHTLLQTGIKSWRLFTITPIGRAANDKDLSLTD
                     EQLKFLMEFIIEKRKEKRMKTSFSCEAWVGRYEEKVRDGFFFCRAGIHIASVLADGSI
                     SACPNVDRSLAQGNIYKDDFFDVWNNRFEIMRNRSWTKTGQCAECKDYSICEGSGLHW
                     WHGDRKEILRCLGKVK"
     gene            complement(34997..35371)
                     /locus_tag="PKY63_03295"
     CDS             complement(34997..35371)
                     /locus_tag="PKY63_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99671.1"
                     /translation="MTSWFKNFRRKLFGFLSFSTALFVFQACYGAPQDQFDDVLVKGT
                     ILSDTTNQPVSGIKVIVNSDSQFALSDSSGAFSFFVPSATQYHLLFSDADTAANGFFL
                     EQDTVLSSIPENGIVIRMTSAN"
     gene            35597..37105
                     /gene="atpD"
                     /locus_tag="PKY63_03300"
     CDS             35597..37105
                     /gene="atpD"
                     /locus_tag="PKY63_03300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPE99672.1"
                     /translation="MAEMTTGKIAQIIGPVIDVVFEREEDLPNIYNALTVERENGETL
                     VLECQQDIGENTVRAIAMDATDGLSRGMQVVNTGKPIAMPVGDQINGRLFNVIGEPID
                     GLPAVNRDKTYEIHREPPKFENLSTASEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVIIQELINNIAKQYSGFSVFAGVGERTREGNDLLREMIEAGLVNYGDEFKHSMEQG
                     GWDLSKVDVEKLKSSLLSMVFGQMNEPPGARARVALSGLTVAEYFRDGDEKSGGRDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKRGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLNRKISELGIYPAVDPLDSTSRILDPNIVGEDHY
                     NTAQRVKNILQRLKELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFYVATQFTG
                     IPGVFVSIEDTIKGFKMILDGEVDEYPEAAFNLVGTIEEAIEKGRKLLEQSK"
     gene            37114..37350
                     /gene="atpC"
                     /locus_tag="PKY63_03305"
     CDS             37114..37350
                     /gene="atpC"
                     /locus_tag="PKY63_03305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPE99673.1"
                     /translation="MRIEIVSPEKQLYAGEVSMAQFPGTEGSFQVLNNHAPMIATIGA
                     GAIRVRNENGNEEMVEVNGGIVEVQKNKILVLAE"
     gene            37431..37745
                     /locus_tag="PKY63_03310"
     CDS             37431..37745
                     /locus_tag="PKY63_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99674.1"
                     /translation="MIRNLLFVVMIFASCAAFSQEQVVDDIQINSDGKTLTIKIDDPK
                     AVKYDLLIYKSYEDIKINDYGVTKNPVTIDISTWEPAIYHIKIDYARITQFRHYEVLK
                     EE"
     gene            complement(37781..39238)
                     /locus_tag="PKY63_03315"
     CDS             complement(37781..39238)
                     /locus_tag="PKY63_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99675.1"
                     /translation="MKFYLLPLLFVLFSGFVSAQKGIGLEGRIFSAENNAPVPGQKVF
                     VVFESSAQPYVQNLHNELITSSNGSFSLSAPNIPNAIIPLDVMVYTYDCDFQRKGYLL
                     TFNYNNVIGEDVNISVCTGSIQLPADMMVSQPADNTCPSFARIYASDSLKRSYLHESY
                     QWTINDIYQGSGNSIDAFFINEQNIVKLTQTFTDSITGFVVDSIQLHENFDIPAAASY
                     ILGGNVLLDGNNTTYGTAVLLGRSNNDYFIIDTASYQQYGYYYFTALPRCNYSVRIIE
                     SDGNLHSNSVPTYLGSVLQWETASYLNLTSDDYCANITLPAQQTGTGIGEISGIINSD
                     DPSGYDVLLYSNNMTPYSFRHCGTDGYFSFSGIPFGSFILVSEKYGVSSISQSITLSA
                     SNPSAFITFSPTTQMNEQEAIAFSVFPNPADDYIILSNKPESEIHIYTIEGKEIMLVP
                     SMDKTIDIRQLRAGMYFLQTMLDGQIATIQFVVSR"
     gene            complement(39238..40539)
                     /locus_tag="PKY63_03320"
     CDS             complement(39238..40539)
                     /locus_tag="PKY63_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99676.1"
                     /translation="MNRLHENDLDRLFAEKFDDLRVEPPERSWKKVAAGIAITTTVST
                     AVFSNLIRPVVWSLSTIATIIMITVSNPSSDSEHSNNLPAAQNIPATNISVERLPMLT
                     AISNIPVPSEQKQVDFIPFVIPQNSAESVETVLHENPALNTVETETERTPELPVLLSM
                     LPTQPISAPHIEWQPQFLSKPNLDFQEKNIIPAWFDLSYQSGPDAFDFGTQNNERITS
                     LAINNGIDISFHFSDFYLRTGVNALNFYQRNRYDYMLNEFAQTGVYTLVDSIWFVQGY
                     DTAGNLVDIPQFSTETYPMYDSVAANYSINANNHYQYIEIPLALGFQKDIHRLSLYAQ
                     GGFSYTFLLKTSELNQAEFSEASGIQPLSWQNQSLSRNKDFWSFTLAGGIVYNTNSRF
                     SFGAESTYRYCLSPLYADSELSGKTPVSYGLKLRLIYKLSY"
     gene            complement(40523..41110)
                     /locus_tag="PKY63_03325"
     CDS             complement(40523..41110)
                     /locus_tag="PKY63_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPE99677.1"
                     /translation="MSDIQQTNTDRSLGEAQLVKNCLAGKAESQELLYRRYASKMFGV
                     CLRYAKNKMEAEDIMQEGFIKVFQNLKNFRCDGSLEGWVRRIMVNTAINYYKSNLKYL
                     QTLDIDDCSNRDSVSVEATDNISLKTLLTLIQKLPEGYRMVFNLYVIEGYSHREIADS
                     LGISENTSKSQLSRARKVLQDKLKTINAVCYEQIA"
     gene            complement(41286..41792)
                     /locus_tag="PKY63_03330"
     CDS             complement(41286..41792)
                     /locus_tag="PKY63_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99678.1"
                     /translation="MKLFTTLACTLIFMQLLAQQSCFLKLTHIDKPEKTTEFRAGDRV
                     IVYFGDTSEICGKIKSIDKSNLVVDSVTMPIHQIQIIVDRKPKSAMFKLAGAGMVLGG
                     AALTVGGFACLAAGLLPPSIKSLYCVPGGLVLDYFGISFIIQGINKLGGKGKQFDIGY
                     RYKLEVVE"
     gene            41944..42588
                     /gene="cysC"
                     /locus_tag="PKY63_03335"
     CDS             41944..42588
                     /gene="cysC"
                     /locus_tag="PKY63_03335"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPE99679.1"
                     /translation="MIKFRRSRAERYANPAQWTDFNSFIRRNRRRFLSQQRPLAIWFT
                     GLSGSGKTTLSDSLNSIILRKGYFTKVFDGDVIRTGLCSDLGFSEDDRRENIRRTAEV
                     ARMFMDSGLIVLCSFISPTHEIRELARRIVGPDRFVEVYVNCPIEVCELRDVKGLYKK
                     YRLGLLKNMTGFDSPYEAPLNPDIEIRTDLWDVNKCSRYLFRQVIKHVKYRKKK"
     gene            42585..43487
                     /gene="cysD"
                     /locus_tag="PKY63_03340"
     CDS             42585..43487
                     /gene="cysD"
                     /locus_tag="PKY63_03340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPE99680.1"
                     /translation="MKYSLNHLQELEAESIFIMREVAAQFENPGLLFSGGKDSIVMVH
                     IARKAFWPAKIPFPLVHIDTGHNFEETLQFRDELVAELGARLVVGSVQESIDKGRAVE
                     EKGPGSSRNILQTVTLLDTIEVNKFDAMLGGGRRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNLFNGRKKMGEHFRVFPLSNWTELDVWMYIRRENIKIPNLYFSHDRPVFKR
                     DGMWLAWAPFMTMKDDEILETKTVRCRTIGDMTCTGVVLSEANCMDEIIAEVAASRQT
                     ERGNRADDKRSESAMEDRKKEGYF"
     gene            43497..44774
                     /gene="cysN"
                     /locus_tag="PKY63_03345"
     CDS             43497..44774
                     /gene="cysN"
                     /locus_tag="PKY63_03345"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HPE99681.1"
                     /translation="MNEEFNSDEYFDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFE
                     DQYEAVKSASEKRGEEYINLALLTDGLRAEREQGITIDVAYRYFSTPKRKFIIADTPG
                     HIQYTRNMVTGASTANLAIILVDARKGILEQTRRHAFIASLLRIPHVVFCINKMDLVD
                     YSKEVYDKILEDFKHFSSKLEVNDIRFVPISALKGDNIVDRSTNMNWYQGPTLLYLLE
                     TIHIASDYDMINCRFPVQYVIRPFSDEHHDYRGYAGRISGGIFKPGDKIMALPSGFTT
                     TIKRIDTFDGPVEEAFPPQSVTLLLEDEIDISRGDMLVRENNVPQVGQDIDIMLVWMN
                     EKALNVGGKYGLKHTSKDLRCLVKEVKYKVDINTLHRNMEDKTVGLNEIARISLRTTQ
                     PVFFDKYRLNRITGSVILIDEGTNETVGAGMII"
BASE COUNT        11858 a         9066 c         9984 g        13936 t
ORIGIN      
        1 gcaagcatgt atgagcagct gctgccgcat acttcaaagc tttatctcac ctgcgtggat
       61 ggggaatatg atacggacac ttttttcccg gagctgaatg aaaaggaatg ggaacttgtt
      121 aaagaatcgg atatcatggc tgattcgaaa agcggcatac aatatcagta tctgattttt
      181 gtccggatat aagttgttcg aagttattaa gttgtgcgat gtttttaaac ttcgcacaac
      241 ttatattggt ctaaaacaaa aataaatcat gaaacaaagc ttcttttttc tgctggcgct
      301 tacattgagt gcgcttacta ttttgtcatc gtgcaagaat gatgaagata atgaagacaa
      361 gaacaactat cggtcagcca ttgataatgc tactgcagaa accatgttca gtgatgtgtt
      421 caagcaggct accgatggaa tgattgctgc gcaggattct gccgaaggca acaagaattg
      481 catggaatcg atggcctcgt gtgctacgct gactattact ccgtttgatc ttaccacatt
      541 tcctaaaacg cttactgtgg attttggtac agtcaattgt atgggcacag atggtcgtta
      601 caggcgcgga gttgtaacaa tgacaacaac gggttggtat cgtgacagcg ggacagttat
      661 cattgtaacg ccgcaaaatt attttgttaa tgatcatctg gtgcaaggaa caaaaaccat
      721 taccaatatg ggacataacc tggcaggcaa tcttgtttat tcccttttta tagatggaac
      781 agttacaaat tccgatggta ccattgaatg gacttcgacc agggagcatg aatggattga
      841 aggtgaaccg actgttttta ttccttggga tgatgtttat ctgattacag gtactgcaga
      901 aggagtgaat ataaatgatg aagcatttga tgttgttgtg aatacggcat tgcgagttca
      961 ggtgggttgt cgttggataa cagccggatc gctgactctt accagcggaa atttcaccat
     1021 gcttgtcaac tacggtgacg gcgcctgcga tgccgccgca acagtaacaa tcaacggagc
     1081 cgtatataac attacgatgt cgtaaaaact tttccactcc ggtaaaactt ttccaaagtt
     1141 tcaaactttg gaaaagttag gagtggaaaa gttttaaggc ggttaaactt caacagccct
     1201 cgcttcgaaa ttttcggtat cgatgtagat gatatacgag gattcaacca tcattccggc
     1261 atcggtcagc agcgttgaat aatgttgtac ctgttcgcgg tgctctttct tttcacggcc
     1321 ggttttaaaa tcgattactt tccatttgtt ttcgtgatcg aatatcatgc ggtcgggtcg
     1381 ccatacgatg ccgtctttat ctgcgatttc cctttcactc atcacaaatt tgcgtccagg
     1441 aaaagcaaat gcaaattttt caacaataac attgacaagc tgaatatatt tttctttcag
     1501 ttcgttttca atccatccgt ttctggtgta gttgtcaata acgtgagtga tgtcggatgc
     1561 ttcagtgatt cctgaaagca attcatgaat gatttttcct ttcatggccg attcgcgcag
     1621 ttcggcactg tcaaatcctg ttttgctttt gcgaagcgaa tctaaaggtt tgtttggatt
     1681 tacccgaaaa gtgtttacat tgaaatgtat cccttttttc tcttttggct ccctttttcc
     1741 ggcaaagttg cccaatgtcc aactggtatg gttttcatga gttgtttcag aaaatttctc
     1801 gtttgtgcaa cggataaaat cctgaagaaa attagccgag tttgtctgag ttgttgtttt
     1861 atttgttggt tctttctttt ttgatttgtt ggattcttta gtggttttgt caggggttaa
     1921 aaacgaaaac aaaaataatt tttcttcagg acgtgtggtt ccaacataca tcagattgat
     1981 agtatccatg agacttttgg ttttctctat ctgatattgt tcacgtatgt cttttcccgg
     2041 agaatctttt gtgtaagtaa caacagagta tggaattccc cattgttttt catttgtttc
     2101 atgccagaaa agatttctgt tgatgctttt tgtttcatca atattaaagt atggcataat
     2161 cacaatcggc cattgaagcc cttttgaact atgtatcgtc ataatgttca cagcatcgct
     2221 tgcatcagga accagaacgc tggtggtttt gcctttttca gtccaccatt gccagagcag
     2281 atcggcatta taaccggttt tgtcttgttg tttcataatg gcttcacgca aaaacaaaag
     2341 aaaaaagcca cctgtgtctg aaatgccaag cgtatgaatg ataatatcga gctgatctgc
     2401 gggcggcata aatacaagtg attcaggatt cagtgcatct aatttctgcg tgagatagtt
     2461 caataacatg atgccacgga catttttttt ctgaatcaga aagttattta tttccttatc
     2521 atcttcgaaa tcgatgttgg catattgttg cagcaggagc catgtttcag tggcattgag
     2581 gtcgtcgccc ggacaatgaa gtaagcggta catgcacatg gcaagtctgc tgtgtggtga
     2641 agtacagaac tggagtgtct cacgcgaaat gaccggaatg gcttctttca gactcatcag
     2701 ataggagctg atagcaatcc cgatgttgtt ttgcctggtc agaatggcga tgtcgctcag
     2761 attgtatttc agatcagtgc attttctgat gatttccaca atgtcttcga gccattgctt
     2821 tttatggtcg ttgagttttc ctttgtagat gcggcattca ataaaaccgg ctgcggcagc
     2881 atttggtttt tgtttaatct gatcgtaaac actggaataa atatcgagtt tcttttttat
     2941 cggttcactc acatcttctt cactcagcga agcaataaaa cggttttttg taaattcata
     3001 aaattgcgag ttgaaatcaa caatattgat atcagatctg taattgtatt ccatgtcttc
     3061 tttttgaaaa gcacttttaa tgatgctttc tgcttccagg gtatcatcac tgccttcaaa
     3121 tatttcggga agtccgagca tgatttcaac tttgccattg cgccagcgat agatgctttg
     3181 ttttggatcg ccaaccaccc atgatgaatt tcctcgtgcc agcgattcat gaattaatgg
     3241 cagcaggttg agccattgca tcagactggt gtcttgaaat tcatcaatca tgatggtttt
     3301 atatcggctg cctgcacgca ggtagataaa tggtaccggc tccttgctta aaacttcgcg
     3361 gatgatgcgg ttgaagtccg ataccggcaa aatgtttttt tcggacttgt actcttcgcg
     3421 gaatttccat atctcgctca gcaaggcgac tacaggaaat cgctttgcca tcatgcggcc
     3481 gtatttgtat tctgaaatca gttcaatgat ttcatacagc agtgtctgta tccggctctc
     3541 aatctcaggt ctgggattaa cgagaaactg tccgctatcg agaattttca tcatggtttt
     3601 agagagctct tttgtgttga aactcgcatt caaactcatg atgtattcaa atattgtttt
     3661 tgatggacct tttttaataa agtctttttc acctatattt tctttcgaaa gaatagagtt
     3721 gatttcatca agttttttcc ggatttcaga tgcaatgttt ttgtttttcg acagtaagga
     3781 atcgaacgct tttatcattt tttccgaacc ggcttccaga gcgctgtcaa cagtgtttac
     3841 cgatgcctcc tgggtcaggt gttgcaccaa cggcagtagc tcatcagcca gtcggagagg
     3901 actgttgtcg tcgtccatct gcatgcggat gatttctttc agcactcgtg tgatattttc
     3961 gttcaggttt gcgttgtcga tgatgtttga aattatcagt tcgtttagtt cctcatctcc
     4021 gacagtcact tcgtaattgg ccggcaattg cagttcgcgg gcaaatgtgc ggatgatgcg
     4081 ctgcataaag ctgtctatgg tgctgatggc aatgtcggag taatgatgca gcacgtattt
     4141 cagcgcccgg tcggccatct ggcttaattc agctgttgtt ttttgcggaa atgcctgttg
     4201 cagtgccgga agcattctgc ttttttcagg agttcgtgca atatctgaca gcgagctgat
     4261 gaggcggtcc ttcatctctc cggccgcttt gtttgtaaat gtgatggata aaatgcgcga
     4321 agcttcggaa ggattgggca gaatgagccg cagcatttca agtgtgattc gctgggtttt
     4381 ccccgatccg gccgaggcgt tgagaactgt aaaggatgac atatgttgat gaatggtttt
     4441 atttgctaat gtagtatttt ttattatacg tattcaacca ttcattctta catttgtcta
     4501 aaaattattc aataattctt gattactttt cttatgcggt cgtgtttgat agttcttata
     4561 tttatcattt cttcggtttt tgtttttgca caatcgttga atgacagctt attgcttcat
     4621 tacccgatga atggcaattg ccacgatgta agcggaaata actttcatgc tgcgttgatc
     4681 aatgccacac tgacctccga tcataacgga aacacaaaca gtgcttatca ttttgatgga
     4741 gcaacagaat atataaactg gccatcgagt ttggttctgc acccggggtt acctattaca
     4801 attgctttct gggttaaata tgattcgaat gattatctca attccttttt atttgatact
     4861 gattttgcgc aggacaatta ctcaggaatt cactgcaatt tgacaccctc gctacagata
     4921 acattttctt ttggtgattg ttccggttca acttcaccag ctgcacgacg ttcaaaaaca
     4981 ggcagctctt ctttacagat aaatacctgg tattttgttg ttgcggttat gcgcggtgca
     5041 accgacatgg atatttacat caattgcgtg aacgacggtg gtacctattc cggctctggt
     5101 ggttctattg catacaccgg tacgcctggt attcttggcc gttcagatgg tgacatgtat
     5161 gcaccggcaa gatatttcat gggttctctt gatgagttca gatactggca tcgcgctctc
     5221 agtgcagctg aaatcctttc gctgtgcaac agcaataatc cggtcattga agccagtggc
     5281 tgttacgacg gtgtttataa tttttctctt caaaatgccg gctcctattc aggagttaca
     5341 tgggatttcg gcgatccggc ttccggtgcg gcaaattcat ccgtgcttga caatccaacc
     5401 catgtttttt cagcccctgg cacatatcag gttactgctg ttgtttctta ttacatcggc
     5461 agcccggtca gtacgaacta tacagtggtt gtgcaggcgg agccggatct gattccatat
     5521 gattcagtgc ttgagtgcgc gggttcatca agagtgattg aggttgaggg catatgggat
     5581 tcgtatcagt ggagcaacgg tgcggtgact cagtcaatag tggtttcagc ttcgggtgtt
     5641 tattctgtca gtgccaccga ttcgaatggc tgcattgtag ctgatacagt cgttgctgtt
     5701 ttcgtcaata caacggtttt tgaaacagac acgacattct gcaacggcag ttccatcgtt
     5761 ttcgatggtc aggtgatcag tgcagccgga gattactcct tcactcatcc ttcgggcagt
     5821 ggttgcgaca ccattatcaa ccttcatgca actgtggaaa acgctcctgc tgtttcgctt
     5881 cccggtgata ccacgctttg tacaggcacc caatttccta tttcacctct tgtttccgga
     5941 tcctgggatg ccggcgcctg gtccgatgga gtgaccacca ccggccggat cattaatata
     6001 ggcggagtct atttctatag cgcaacggga atgtgtggaa cggcagaaga tgaaatcata
     6061 gttgcttttg aggattgcgt cagcgggatt atcttcccca atgtattcac tccgaatgga
     6121 gatatttaca acccggtatt cagacctgat ggatttaata tcgaagattt ccatattatc
     6181 gttttcaatc gctggggcgg tcaggtgtat gagggctttt ccatttcaga tggatgggat
     6241 ggcactttta aagggcagga atgtccggaa ggagtctatt tctggattgc tgaatacaaa
     6301 gtgtatcaga ataatgtaat ggttgcagac tcagcaaaag gcagcgtaac tcttttacga
     6361 taggtgtagc tgcaatataa attcaatgtc tgtgttatta ttcttaattt tgccgttatg
     6421 gcaaaataag gcgtaattca atggctcagg gaatttcgaa atcatttata aagtcttcgt
     6481 tcatctactc cttcattggt gcattaccac ttgcatcaag tgttctgttg ctgccatttt
     6541 atggcaactc ggatttgttg tcaaccagtg attttggttt actggccatt tacattgctt
     6601 tgagtgaact ggtgaggatt ctttttatat attctgctga caattatctg ggaatcaact
     6661 ttatacataa ttcagattcc cccgcccaga cacgtaaatt tattggaacg tctgcgttgt
     6721 ttctgattat ttttggtgtt ggtatgactc tgcttttttc ggttgccggt gattatcttt
     6781 tcacatttat ttttcccggt aaggatgtca acttttaccc gtatggactg ctttctattt
     6841 taacgggatt gtttaccggt attttcaggg catataccag tctgatgatt tatcgtcaga
     6901 aaccaaatcc ttatttctgg tcgaatatgt tgcatttcat tcttgtgatt gcattttcgg
     6961 tcagcggatt gtatttgttc ccaatgtcgc ttgaagggcc catctggggg cgtttcattt
     7021 cagccgtatc cacatttatc tgggcaatgg tttactttgc taaagaaagc cattttgagt
     7081 attctaaatc ggtgcttaaa gatctgttaa ggtattgcac ccctttatac attttttaca
     7141 ttctgacatg ggttatttcg aatattgacc gctattttat tcttggcatc atgacagaaa
     7201 aagaggtggc cattttcgac tttgctgtaa aaattacgct cattgtggaa ctcttacagg
     7261 ccggtttatc ggctgccatc aatcccaaag ttttcaaaat ctggaaagaa aatggggaca
     7321 gaccagttgg taatgttgaa attaatcgat atttcaacgg ttttacagtg ataaatcagc
     7381 tgagcattcc tcttatttat gtggctgttg tttttttggt accctttgtt gtctccaata
     7441 cagatttgta tttctcgttt aatctgcttc ccgttctgct ggctggtatg gcaaccaggg
     7501 tctggttgat gtatctgatg actcctattt attatttcaa aaaaacaaaa atgctaccac
     7561 tgatttatgg cttagtagct gttattcagg tgcttgtaac ttatatcttg atacgtttca
     7621 atggaattga aggtgctgca tgggccaatt ttttgactaa aatggtgcag gtgtttcttg
     7681 tatttatttt tgtgagaagg ttctatactt tttcagtgaa tccgcaaaaa ttcattgttt
     7741 atccagccat atacatcctt attttgctgg caacagatct tttcttcagc gatatcaatg
     7801 tgtatctgat ggcttcgctg catcttgtta ttttgtctgt tgtcgggttt ctgatgttta
     7861 aaaaggaaat tgtaccgttt gtaaaatctc tgtatcaggg tcgggtaaaa tcgtaaaaaa
     7921 actcccgcag gtttgggcca atgcgggagc ttaagggtat attcacaact tgttttttat
     7981 ccgagatatc ctttcaggtt tcggctgcgg ctgccatgtt tcaggcggcg gatggctttt
     8041 tccttgatct ggcgtacgcg ttcgcgtgtg agaccgaatt tatcgccgat ttcatcaagg
     8101 gtgagctgtg cagcgccatt caatccaaaa tagaatttca acacgtcgcg ttcgcgcggt
     8161 gtcaatgttg caatggcgcg ttcaatctca atattcagtg actcgaattg cagacctttg
     8221 tcagggcgtt cactttcctc gttggcataa aggtcgtaga ggttggtgtc ttcgtcggtt
     8281 acaatgggag catccatgga gacatggcgt ccggtatgtc gcagactgtc ttttacttcg
     8341 gtatcgggca tgtcgagaag agcagcaatt tcgtgtgcat ctggttcgcg ctcgaattcc
     8401 tgttctaatt gagcaaatgc cttgttgatt ttgttgatgg taccaatctt attcagcgga
     8461 agacgaacaa tacgtgattg ttcggccaat gcctgaagaa tggattgacg gatccaccac
     8521 actgcatagg aaataaactt gaaaccacgt gtttcgtcga atcgctgggc agcttttatc
     8581 agtccgaggt tgccttcatt gataaggtcg ggaagactca gaccttgatt ctgatattgc
     8641 ttggcaacgg atacaacgaa gcggagattg gctttggtca gtttttccaa agcagccata
     8701 tctccctgct tgatgcgttg agccagcacg acttcttcat tggcagtaat cagttcttcg
     8761 cggccgattt cctgtagata cttgtccaat gaagcagttt cgcgattggt gacctgcttg
     8821 cttattttaa gttgcctcat aaaattctct cagggttggg ttagcattta tacgatgcgc
     8881 ccatacgaat gttacacaaa aaacgtaaaa ttatttttct tctttaggta acagcacttt
     8941 acgcgaaagg cggagttttc cggttttttc atcggttcca atcagtttta cctgaatttt
     9001 gtcaccaact ttcagcaccg aatttacatc ctgaatgcgt tcccatgcaa tttctgaaac
     9061 gtgcaacaat gcttcttttc ccgggagaat ttcaacaaat gcaccatagg cctgaatggc
     9121 ttttacggtt ccttcataca cttctcctac ttcaggaacg gctacaattg attttaccca
     9181 attgtgtgct gcattcatac tctccttatc ggctgagaag atttcaacaa caccaaagtc
     9241 gcccacttct tcaataagaa tggtggctcc ggtctggcgc tgaatttcct gaataatttt
     9301 accaccaggg ccaatgactg cacctatgaa ttcgcgtgga atgctgaatt tgacaaaacg
     9361 tgggacgttt ggtttgtagt ctcctcgcgg ttcggcaata gttgcggtaa tgattttcag
     9421 aatatgctcg cgtccacgtt tggcttgtgc cagtgcttcg gccagaatgt catacgaaag
     9481 tccgtcaacc ttaatatcca tttggcaggc ggtgatccca tccagggttc cggtaacttt
     9541 aaagtccatg tcgccgaggt gatcttcgtc gcccagaata tcgcttaata cacaataatt
     9601 gccggtttct gaatcagtaa tcagccccat ggctattccg gaaaccggtt tgcggagttt
     9661 cacgccggca tccatcagtg caagagttcc tgcacaaaca gtagccatag aagagcttcc
     9721 gtttgattca agaatatcgc tgacaatacg gatggtgtat ggattggaat catcctgtgg
     9781 aatcatattt ttaagagcgc ggagggccag atttccgtga ccgatttcgc ggcggctggt
     9841 gccacgcagc attttggctt ccccagttga gaaaggcggg aagttgtaat gcagcaaaag
     9901 tttgtttgtg ccttcaaaaa cagctccgtc aataatctgg tcatcgagtt tggtgccgag
     9961 agtaacagtc gttaaactct gtgtttcacc tctggtaaaa atggcacttc cgtgtggagt
    10021 aggcagtggg ctgacttcgc accagatggg acggatttca tccattttac ggccgtcgag
    10081 gcggatacgt tctgccagat acatgtcgcg gaccgctttt ttcatcacat cgtgatagta
    10141 gcgggcaacc aatgattttt ttgcatccag ttcctcttcg ggaatggttg cgagaaattc
    10201 ttccccgatg gcgtcgaaat catcctgacg ctgatgtttg ttgggagtgc agcgtttggc
    10261 tacttcgtat gcttttgggt aacaaaagtc ataaacggct ttgcgaagtt cctcatcgtt
    10321 cacttcgtga caatattcac gtttcgggtt ggctttctct acttttgcag cgagttcaat
    10381 ctgagccgca atttgttttt taatggcttc gtgagcaaat ttaagggctt ccatcatcac
    10441 ttcctcgctc acttctttca tttcgccttc caccatattg atatctttgg cattggcggc
    10501 gaccatcaat tcgaggtcgg cgttttcgag atctttaaca gagggattga ttacaaattc
    10561 accgttaatt cgtgcaacgc gtgcttcaga aatcggtcca ttaaaaggaa tattggaaac
    10621 agctaaagct gaagaagctg ccagagctgc ataagcatcg ggatgggtat cggtatcgcc
    10681 cgaaatgagg taaatgagca cctgggtttc ggcatgataa ttatctggga aaagcggacg
    10741 cagagctcgg tccacaaggc gggcaatcag aatttcgtat tccgaaggac gcgcttcgcg
    10801 tttgaaaaat ccaccaggaa aacggccggt agaagcatat ttttcctgat attcaactgt
    10861 gagaggcatg aagtcaacgt tttccttggc ttctttgttg gaaacgacgg ttgccagcag
    10921 catggtgtca ccaatgcgta caataacaga tccgtcggcc tggcgggcca gcttgccggt
    10981 ctcgatagtt ccggttcgcc cgtcgggaag ttcaaatgtt tgtgtgatag cgttcatctt
    11041 ttttgtatgt gtttaaaaaa aagaaaggca atacccgtat tgcccttccg ttgtaaaatg
    11101 tcagtatgac tactttctga tttccaattg ctggattaaa gtacgatact tttcaatatc
    11161 tttggttttg atgtaggtca gaagcttttt gcgctttcct acaagagcca gaaggctgcg
    11221 ctcggtcaca aagtcttttt tgttgctttt caggtgcccg gtcaaatgtt tgattctttc
    11281 agtgaaaata gcaacctgta cttccggtgc tccggtgttg gttactgctt taccgtattt
    11341 ttccgcgagt tgttttttgg tttcgattga taaattcatg gttctctttt ttatcgggct
    11401 gcaaaggtac aataattatt taatattcaa aacttttatg gagaaaaaat tcttttctga
    11461 acacattata gatattaaat gctttcgtcc agcacattct gccgacagct gactactgac
    11521 cattcacagc tcaccactca ccgctgacta cccactaccc actactcact gctcaccgct
    11581 cactgctcac tgctgactgc tcactgctga ctgctcaccg ctcactgctt taaatggtcc
    11641 cgttttcaat catcaccaca atggtttcaa tatcgtggtc aactcccgct gggtcgtagg
    11701 taaccttgag gttgtaaccg aagatttttc cgaaagtttg ccagaaagtg gctgtaatac
    11761 cccctctgaa gtctctgata aggtgatagg ttgtattgtt tgtttccctg taaataagtt
    11821 tcatcagaat ttttccctgg gtaaaagtca atttcaccag ctgactttcg agttgtgcgc
    11881 gcatttcttt ttcaatccgg tccagttctc gttctctttc acggtcattt tcaatcccat
    11941 tgaggtattt gttgtattca tttactttgc tgccaatgag tttggcatag ggatacacac
    12001 gtttgatatg acgtaccatt ttggtgaatt ctatcacctg cacctttgat ttaaatacga
    12061 taggaccata aatcctgact gttgggatcc agaaaagcgg aacggtatcg ccttcaataa
    12121 ttacagcttt tgttacaatg ccatcttcta tgccaccgcc ggtgtttgat ggctgagcca
    12181 gagcaagcat gcctgctgca acaataaata tcaccagtat aagcagcttt ttcattttgt
    12241 tttttgctct ttatacaatt ctttggccag attatgaagc aaccttgagt tgaatttcag
    12301 gatgattctt taatttgttt ttgacaaaag ttgctgtgat ttttattttt tcatttttct
    12361 cgctttcaga caagtcaaac ataagatcgg tcatgatttt ttccataatg gatcgcagac
    12421 cgcgtgcgcc aagtttgcct ttaatggcca tatgggcaat ttctttcaat ccatcgggtg
    12481 tgaactcaac ggctacattg tccatttcga aaagggcctt gtattgttta acgagcgagt
    12541 tttttggttt ggtcagaatc tcaagcatgg cttcttcact tagcggatgc agatgagcca
    12601 gaaccggtaa gcggccaaca agttcgggga ttagtccgaa tttgcgcaaa tcttccgaag
    12661 caatgtattg aagcaggttt tctttatcta tttccttctg ttcgcctttt ttcttgaatc
    12721 cgatgaccga tgttttcaga cggtttgcta tcattttttc aattccatca aaagcgccac
    12781 cgcagatgaa gagaatattc tgtgtattga cctgtatgta tttctggtcg gggtgttttc
    12841 ggcctccctg tggcgggaca ttgactaccg ctccttcgag cattttcaga agcgcctgtt
    12901 gaacaccttc tccgctgaca tcgcgggtaa tgctgggatt gtctgattta cgcgaaatct
    12961 tatcaatttc gtcaatgaaa ataatgccat gttcggctgc tttcacgtcg tagtcggcca
    13021 cctgaagcag gcgagccaga atgctttcca cgtcttcgcc cacataaccg gcttcggtaa
    13081 gcgctgtagc atctgcaatg gcaaaaggaa catcgaggaa tcgtgcaatg gtttttgcga
    13141 gcaatgtttt accggttccg gtttcgccga ccaaaacgat gttgcttttt tcaatctcaa
    13201 cgtcgttttc ttttgctctg attcgtttga aatggttgta aaccgcaaca gacagaagtt
    13261 tttttgcctg ttcctgacca atgacatatt gttcaagaaa attgaatatt tcttttggct
    13321 tacgtagggt aaaacctgcg ccggaagccg gttttttgtt tttcagtgcc agctcttcat
    13381 tgataataag ctgggcctgt tcgacacatg catcacaaat gttgccactt acgccggtaa
    13441 tcagcagttt gacttcctgc tggctgcggc cgcaaaagga gcaatggtcg gttggttttg
    13501 ccatgatttt ttattttttg tcacgacgaa gtacttcgtc aatcattccg tattcttttg
    13561 cttcatcagc tatcatccaa tggtcacggt ctccgtcttt ccacacttta tcatagggct
    13621 gcccactgtg attggcaata atctgatata attcttcttt gagcttttta atttcagtgg
    13681 cgacaatttc aatatccgag gcctgtcctt cggccccgcc catgggttga tgaatcatca
    13741 cacggctatg tgataatgct gtgcgtttgc catgtgtgcc ggcgcagagc agaacagcgc
    13801 ccatcgatgc agccatcccc gtacaaatgg ttgcaatgtc cggggcaatg tactgcatag
    13861 tgtcataaat gcccaatccg gcataaacag agccaccggg cgtattgaga taaatagaaa
    13921 tatctttggt tgcatcaacg gattcgagaa acagaagctg ggcctgaatg atattggcta
    13981 cataatcatc aatgggtacg ccaaggaaaa taatacgatc catcattaac cgagagaaaa
    14041 catccatggt tgcaatgttc agctggcgtt cctcaatgat ggttggagaa atgtagctgg
    14101 atgaaatttg ggaatatttg tcaaacacat tgctgctgat gcctcggtgt ccgatagcat
    14161 attttctgaa ttcgtcgttc atgttcataa tcttagtttt tttcgttcac taaattaaga
    14221 aaatctttcc agttcattgt ttttgcttcg atggtgcaat tatcttcgaa aagtttaata
    14281 atccgttcat ctgtcatatt gttaaccaga ttgctgaaac gttccttttc ctttgaaatg
    14341 atgtcataca cctgatcgat tttgtttttc acatccggat cgttttcgtc catgtcggcc
    14401 agcccgagaa gttctttcaa atgctgacga atgtcttcgc gggtgatgag cagattgtat
    14461 tttttgatta atttgttctc aataagctgc cacttcaatt catcgatgta gaaaggcaat
    14521 cctttttcaa gatcttcttc gctgagttct ttttcagagc gggtgcggat gaattttttt
    14581 aaaaattctt caggtaaggt gaagtcgtat tttgttttaa gttctttctg aacgtcgaaa
    14641 tagaattgtt tgcgggcttc tttttcatag gttcctttta catcattgcg aataatttca
    14701 cgcaactgat cttcgttttc aatttgtctt tcagggaaaa cctgggcgta gagttgttcg
    14761 ttgagctctg ccggagtgat tgaaagcaca ctctgcagcg taatccggta ggtccctttc
    14821 atcgcgcggg catcgtcgat attgatgttg aaaatttcag tgcgctcgtc ggcattcggg
    14881 aaatcttttt caacatcgaa atcaataaaa tcttcagctt tctttccgat gaaaaacttt
    14941 tgagcatgtt ccggcaatgt gtttatgcgc aggtggatga atttatccaa tccatcttct
    15001 tttttttctc cgttttcggt ttgaatcaat tgtgcagaaa caaaacattt ttcatcaact
    15061 gattcggcct tttcaaattt cccgtatcgt tcgcgaattt cgccgagata tttttcaaca
    15121 tcggtttcat cgtggattat ttcaaatttt gtgaaagtca gcgatttgtc tgctgttggt
    15181 tcaaattcgg gttccaaagc cacatcaaag ttgaaagtaa aggtattgcc ttcgttccat
    15241 tcagtcaggc gggcagcttc ttcgtccatc atgggtgaaa gaatgaagtt gaccttattg
    15301 tcatcgagat atttataaac agcttcggat gcaacatgca gcacttcgtc agccaggacg
    15361 gagttcccgt acattttttt tataacgcca aagggaactt taccggggcg gaagccaggc
    15421 atgcttgctt tgcgacggta ttcgcgtaat ttctcttcaa cctttggctg gtaatcagct
    15481 ggttcgatat gagttttgag agtggcaatc aattcaccgt ttttttcaaa ttcgaaattc
    15541 atacaacact tttttgagct ttggaaaata attttgtgcg gatgaaggga ctcgaacccc
    15601 cacgccttac ggcaccagat cctaagtctg gcgcggctac caattacgcc acatccgcaa
    15661 atcaggagtg caaagttaca attattattg gaatcgcaaa tgactgctgt atgtttattg
    15721 tcagtctatt aatgcagaga gggtgtttta gatggtcagt aatattttaa aggagtcaga
    15781 gattatcatt ttaataacag gaaataaaaa gaagccccgg ttctccaggg cttctgtgca
    15841 taaatgtgtg tattggaata aactattctt tgaaaaatct ggcaacgcca tgggtgtcgc
    15901 tctgaatgag atacaatccg ggacttaacg cactcacatc aatactgagt ttttcgctgc
    15961 cttcactttc ttgctgcatc aattggcggc cagcaaggtc atatattgta acaaatcctg
    16021 acccgtcagt cagtacattc aaaatatttc cggcagggtt gggataaatg ttcaggatcg
    16081 ttgttccatc atcggttatg cctgacggat cggtgatagc aaagttgtca atgtcaacaa
    16141 aataggagcc ggagccgctg taggccgata aatacacagc ggtcattcca cctgtaaagg
    16201 ccccgatggg gatttcgaca ctcacaaaat cggacgatgc tatatgattt gcaggattga
    16261 ttgaatgtat taatacataa tccccgctga tcggatcatt cattttaatg tttattgaat
    16321 caatttccga agccggtgtt gcggtattcg gataaccgga aatatttaca atccgataat
    16381 cgaaagtcag tatggagttg cttgtcaaac cacaaaaatg aggtgaataa agatgtttta
    16441 cggtgatagc aggtgttagt tctgccgaaa cgccctgatc ggttaaaccg tggttaatca
    16501 gattttccca acctcctgaa aaaccatcag gcaattcagg acccccatca aaatttgttg
    16561 aaaatggaat gctgcgttgc gaaaaaagat catcaacatt tcctgttgca ctgtaaataa
    16621 gatattcgtc gtatgaccat gttttataat aaacggtttt attcgattcg atgcctgtat
    16681 gaattacttg tccggttggg ccgttataga ttatttccga accgtcgctc agcgtatcac
    16741 cagacaggta aggttggcca caatagggcg agtcaaatgt gccggtataa ttgtaaacaa
    16801 caatcaaatt gtcattgact ccaaattcgg ctgctgttaa aaccgattcg ccacaatcgg
    16861 ataaagtccc tgaaataatt gagggttgag gtgagtcaaa tttcagtaaa accactggcc
    16921 ggtaatatga gagctgactc atcatgcttg ttggaaaact attgtcttgt ccccatgtta
    16981 atgcaacata atcggttgcc gttgttatgt tttcgcagcc aaattcagga acagttgttg
    17041 ggcctgtgcc gccccaggca gtagaaatat aaactgccag atgataactg ctgtcatagt
    17101 caaaatcatt gtccagattg aaataattcc agccggtatt gttgaataaa accttttgct
    17161 ggaagacctg ggtaccattc attgaaacta ttccaaaagt attgttgcct ccaaaaacag
    17221 cattgtttac ttcctccagt cttattgtaa ccgtaacact gtcgttgcaa ggtgctgcaa
    17281 caaagtatgc aatttgttca atggttccgg tcactccaat gttttcctta ttatatattg
    17341 atgcgtgata ggagtagcca tagctgccat cgaacggata cactttttgt gtagggtttc
    17401 catcgccttt gggataaata acatcgggtc ctaattgagc tgatgtcagc aaagaaatga
    17461 acagtgcgat aaatgttagt gtaattgttt tcatcggttt tgtgtttttg tttgtcgcaa
    17521 aattatgacg gtttgttttg tgggcttatc cgtaatttta cggattttat acgttcacaa
    17581 tccgtaaaat tcctgatgaa taaataatca gattcaacct ttctttgtaa caaaaaaaca
    17641 tgaaaacaat ttttctttct gtggtgttat tggttctcgc gcttattggc aaagcccaga
    17701 ccccgattga tgagggttgg gttgaagttg gcaatgctgc atacaactcg atcatatatg
    17761 atgttgtttt taacgatgca aacaatggtt ttgcagttgg ttcaggtggc acttttctga
    17821 aaaccaccga tggcggtgac acctggaatg ctatctacat tggcgtgaat tacacattgt
    17881 ttgaaatagc attcagtagc agcaatgtag gttacatgcg aggcggtaac aaagatggcg
    17941 gcggctattt tggtaaaatt ctgaaaacca ccgatggtgg tgtaacctgg acagaggtct
    18001 tgtcagaccc gtccactttt tttgaaacca tgagcgtgcg ctcagattcg gttgtttttg
    18061 taggtgatta cgagcaaacc caacacaccg tggatggtgg tgcaagctgg attattactc
    18121 cacttaccgg attatacacc attgctaaaa ttggttttga gcaatccggg aatggctttt
    18181 taattgccaa caatagtcgc ttatatttgt ccgatgatga tggccagacc tgggattccg
    18241 tattttattt tcccggagac acgccgcttg attttagttt tgctgatgct gccgatggcc
    18301 tcctgatgtc gtacaaccgc aagctggcta tcagtaacga ctctggtgct acctgggctt
    18361 tacataagac cctcacagaa atgatgatag accttgagtt ttttaatgat tcgactgcgt
    18421 attttttgaa aaactacgga aatactattt ataaaacaca ggatacagct tctacactgc
    18481 agatttcgta ctcaaacctt caggcagacc tgaatggcct gcacaagatg agcgacggca
    18541 gcctgcttgc aacaggtgga ggcggtttaa taatccgcac tatagatggt attatctggg
    18601 atactttgca tatcggagaa atgaatacaa atctgactga tgttgttttt attgacaatc
    18661 agaccggctt tgcagcaggc agcaatggct atatccgaca gactacagat ggcgggttca
    18721 cctggattac atcacaaata aatgcaggaa ttgatcttgg cggaatctgc gcggccacat
    18781 ctaaccggct tattgcagca ggcaacgaaa catcctgtta tatttcaact gacacaggga
    18841 tgacctggac tcagcatatg tatggtgttg atatgggtct ggcacggggt ataatgcgtg
    18901 catcggccac tcaattgttt gctttcggct ccgaaggaat cttcatgacc aatgacgctg
    18961 gcctgacctg ggccgaatcg ggttcttatt ctcaggttta ttcaggattt gttttaaata
    19021 ccgattcagc ctttttcggt tttcaaaatg caggtatcag atatacgttt gacggtgggg
    19081 taaactttac agatattata accgtcaatc cggccatcca gggaattcat ttctttgatg
    19141 cggataccgg attatttgta aactcatggg gaagaatgta ccggacaaca gatcggggag
    19201 ttaactggag tcaggtactc aacagcggaa tcagtctgca ggagttgtac ttcatcgatg
    19261 atacacttgg ttatgccatg ggaaactcgg gccggttgta taaaaccact aatcagggaa
    19321 tcaattggca gctgattgaa accggcacga caaggacttt gtataaaatg tggtttacgc
    19381 ccgatggcac cggatttatt gttggcgacg atggtattat tctgcgcaaa gctgcaacgc
    19441 cagtgttcga catacaattt actgtcacaa acgacagtgg cgatacactt atcaatgccg
    19501 ccatgacttt caattcggtg gcataccctg ccggaatcaa cgaagtttcc gggttaaaca
    19561 gtggttcgta caattatata atcagttgtc ccgggcatct gagtgatacc ggctctgttg
    19621 ctgttgtttc agacactact attgttattg aactgaaaaa attccacacg gtaacttttc
    19681 agctgaaaaa tgtttttaat aatccggtgt ctgcagcagg cgttttgttt aatggtgatt
    19741 cacttgtgtc gaattccgct ggtgatgctg ttttcggcaa tgtgctcaaa tcgccgtcac
    19801 ttccggtaca ggtgaatgaa agccattatc tgccattctc ttcggccaca ggtattgcaa
    19861 atgatacaac catttcaata cttttgcagg ccgatattgc tgctcctgtt ccaaatactg
    19921 ttacggtcat tgacgattat tcctttactg caagctggac agctggcacc aatgccaccc
    19981 aatatgcttt gtttgtttcg gatgataatt ttgtttcaac gctgcctgga tacgatagtg
    20041 tcatcgtttc ttcactcaat tatcctgtta ctgggttgac agcaggacag acttattatt
    20101 tcagattaag atctttaaat agttatggat attccgctta ttcttcagtt ggaacagctg
    20161 aaaccaccac tggaattaaa gaaatttctg attgtttgat tgaggttttt ccaaatccgg
    20221 cgaacagttt tgttgttgta aatccgctcg gggtgatgtt tccgcagcgg gttgatattt
    20281 tcagtgtcag tggacaactg gtgatgtcgc atgagattaa tggtattgaa atcattgata
    20341 tatcggctct gcagccggga gtgtattatt tgagaattat ggattatgtt cagcaaatta
    20401 ttaaacaata atccgatttc catgaaagaa agttcgacgg tagaagaggt tagaaaaaaa
    20461 atgcgtaagg tgcaaaggtg tatccggcag aataagatac aggaagcatg gagcaacctg
    20521 gacactattg atgcatcgat gattgagcag tgcggcgagt ccgagaagca gttaatttca
    20581 gaagctcgtc agatactttt acacattatg gttcgtgaac ttaaagaaaa ataaaactac
    20641 tatgaaaaca ataattcttg cattcacatt gattgttcct ttgcttacag cggcgcaatg
    20701 gaactggctt aacccgcagc tgcagggcga tccgattaac gacatcgttt ttgtttcggc
    20761 caacgatggt ttcgccatca ctggtggagg agctcttatg aaaacaacag atgccggaac
    20821 aacatggaca atggaaggtt gcattgctca gggctatctc accgatctgc atttcggcga
    20881 cgctacacat ggctttgcat gcggttctaa gggtgtattt gtgcgaactg tcgatggcgg
    20941 caacacctgg aatgtaatca acactacatt caacagcgtt gatttttctg gcatctggtt
    21001 tctgaataat actaccggat ttatcattgc cgactacggt gtaatgataa aaacaacaga
    21061 tggcggcgac acctggaccg ctgtgagcac cggctcgaca gaaactttta ccgacatcta
    21121 ttttccttca gcaaccaccg gatatgtttc tactgaagat ggttatttta tgaaaaccac
    21181 tgatggtggt gccacctgga cagagcttga tacctatcag gccgaaacat tcaaatgtgt
    21241 ttggtttacg gatgaaaata caggatggat ggccggaaac tggggtgcca tttataaaac
    21301 taccaacggc ggcaccagtt ggaccagtca atcggttgct actcatgatt atttcaacgc
    21361 catttgtttt gtggatgcct cgacgggtta tttgtgtgcc gacggaactg tttttaaaac
    21421 caccaatggt ggtacaacct ggacacagtg ctataatccg aattatgatt atttgtattc
    21481 catttatgcc gatgcatcag gacgcgtgtt tgccggaaac gaacgtggtc ggctgtatcg
    21541 ctctgttgat gccggaaaca cgtgggctga tctgaccacc gatcttatga ctccatcggt
    21601 aagactgaat gctgttgcgt ttttcaacga tcagaccggc attgtggcag gctcgaatgg
    21661 tgttttgttc agaaccgaaa acgcaggtct tgcctgggac agcattcctg ccggaacttt
    21721 tcaggaaatt tatgattttg agttggtgag caataccgag gcctatgcaa gtgcaggtgc
    21781 cggaacaatt ctgcacacgt ccgatgccgg cgaaacctgg acatccattg tttccggaac
    21841 aaccaatact ctatacggca taaaaaaaat aaacgcttca tttgctgtag catgtggatg
    21901 ggctggcacg gttcttacca gcgccgatgg cggatcaacg tggaccagca tcagcaccgg
    21961 gtttacaaca ggattgtacg atattgattt ttataatggc agcacaggac tttgtgttgg
    22021 acaaagcgga aaaatttacc ggagtaccga tggcggacaa acctgggctg caattacatc
    22081 cggtaccagt caatacctga atgcagtgtg ctttgttgac agtttgcatg tggttgccgg
    22141 caccaatacc cagggattgc tctattcatc cgatggaggt cttacctggt cagatcctgc
    22201 cacaaacgtg gatatgggca ttacgcaatt gtattgtcct tcggctgata ctgtgtttgc
    22261 ttgtggcatg gatggcttga tggccttttc caccgacatg ggactcaact ggacgggcat
    22321 ggatcgtatg acctactggc tgctgaatga tatttcaaaa acgccttccg ggaaaatgtt
    22381 tgcagttggt tacaacagca ctattctatt tcgtggtcag ctgcaaagtg tcggatttga
    22441 aaactttgtt gaaaaggccg atctttcatt gcatgtttat ccgaatccgg ccgctgattg
    22501 tatttatatt gaaaacgggc atcagcagtg gtatatgatt tttgatgagc agggacgaat
    22561 gattcagtcg gggtatgcat cagagaattc aattgatatt cgttcgctgg cgccgggaaa
    22621 ttattttatt caatgttcgt cgtctgagtc tgtgcaaacc gggcgtttta taaagaagtg
    22681 atttggttct ttcaatttcc gaccggagtc ttttgtgcaa gcagaagatt ccggttttta
    22741 ttttccggcc tgttaaattt gtgcgttggt tttacaatta tgttatattt gccagttagt
    22801 tgccttttta tatatgacaa atctgacaaa gaggattgtt tttgcagtat ggttgacaat
    22861 catattttcg ttggccggta ttggacagaa tgatgctgta cttgattcat tacttgaaca
    22921 ggccgccgga gagcgtcgta atggggctta tgacaaagcg ctgaatctag atttcacagt
    22981 tcttgccttg gcaaaaaata tgggtaatga cgccatgctt gctgcagcca ataatggaat
    23041 agcaacagat tatatgcgca ttggtgatct gaaaaaatcc cggatctatt atgaacaagc
    23101 tttggaaata tacagacatt tgcgcgacac tatcaatatt ggtgatcagt attataaact
    23161 ggggtcgctc gatgttgatg aggggaaaat ggattttgcc cgtgagaagt atagcttgtc
    23221 cgtggctgtt ttcaggaaaa aaaattatta tccgggcctg gctgatgttt acaatggtta
    23281 tgcagctatt ttttatattc aacaaaacct ggattctgta gcatggtatg cagagcaatc
    23341 gctgaaatat tacagacttg caggaaatac ggatgccgaa tcttttatgt acatcaatct
    23401 gggtgcactt ttcaatgcac agaaggatca tcctaaggcc attcaatatg tcaggcgtgg
    23461 aattcagctt gccgatagca gcgggcttat gaatcaaatg cgtcagggat atcgcaatct
    23521 gagtgaaacc tatgccatgt taggtgactg gaaacaggcc tatgataacc agttgttgta
    23581 tgtcaggtac aatgattcca ttttcaacca gcagaaaaac gaagctatgg tgaagtacga
    23641 agccctttac cagacatcgg aaaaagagcg caagatacag gaacaaaatg ctgatttgct
    23701 ttcaaaggaa aacagaatca aatcaattca aaatacaaga aacattctta ttcttctttt
    23761 tattgttgca ctgattgtcg ctttattttt cgtttacagg tatattacac gtaaaaaaat
    23821 caatcgcttg ctggatgaaa agaacaagca gctcgaagaa atcaatgcat tcaaagaccg
    23881 catctttgct attatttcgc acgacctgcg ttcgcctgta acatctttca gccgaattac
    23941 ttctgcgctt aatacagcca ttgatcaact tccaccaaag gaaattaaaa aatatctcaa
    24001 cgacatcgag gtctctgcca ttgaattgca aaatatgttg cgtggtttgc ttcattggtc
    24061 tctcacacag caaagcggtg ccaatgtgca gactcaggcg gttcttcttt ctgaatgcat
    24121 tcaggatgtt gtcgccgatt gtcggggagc tttcagcgaa aagcaaatca gtcttgaaac
    24181 tgaaatccct gacagtttga ctgtactgag tgataagggc ttattgcata ttgttttgcg
    24241 caatgtgttg tcgaatgctg tgaaattcag tgatacaggc gaccggatag ttatcagggt
    24301 gacagaaagt gatggaaaat gttttattga tgtttgtgac aatggtccgg gagtaccgga
    24361 cgaagttgcc tcgcgtttat ttgggacaat ggcttccgat gcgccctcac gtggtggtgg
    24421 ctttggtttg tttatctctg ctgagttgct gaaaaaaatg aatggcagca tttgtttggc
    24481 tgcgacatcg gctgatggaa gtgtatttcg tattgtttta cccattgcca accttgtgta
    24541 atatgaaaac aagaattgcc attgccgatg accacaccat tgtgcgagat ggtatccgcc
    24601 ttatgtgttt gaccgatgat gcattggaac ttgtttttga atcggcaaca gtaaatgggt
    24661 ttgtgaaatt gatggaaaaa aactcatttg atgtcgctgt cattgatatt caattcccgg
    24721 atggttctgg tattgatttg gcgaaaacaa ttcttctcaa atttcccgac gcccgattgc
    24781 ttgttttttc aggtatgatg gatgaagaaa ctattttaaa agccatctca accggcgttt
    24841 cgggctatgt ttcgaaagat gcatctatgg ccgaactgag aactgctatt tcaactgttg
    24901 catcttttga acagtttttt agtatgagtg ttggacagat tctttataaa agctatgtga
    24961 aaaagcaaca gcagggacta tctgaaacca tccctgatgc tgtattgagt gatcgcgaaa
    25021 ccgaagtact gaagggcttt gccgaagggc tcagttataa ggagattgcc gaaaaacttt
    25081 ttattagtcc acgaacagtc gaaacgcata aagaaaacat catgaaaaaa ctcggtctgc
    25141 attcgttggc cgacatgatt cgttatgcga taaaagaaaa aataattact ctttgaacca
    25201 agcgtgtatt atcttttaaa actccattct ttttttgaat ttagatccag aattccgggt
    25261 ttttcggagc gtacttttgt cattatgcat ttggccggca tggctccgtt gaatttctca
    25321 aaataatatt tactaagcga cggagccagc atcagatcgg tctgtggaag attctcaact
    25381 gccggtggca tttttcgttc gttccagttg agcatacgga gctggttcca tccgataaaa
    25441 ttttcatgac gaaaaaccat tttgtcattg aacatttcac cggcagtgat tgtttgtaag
    25501 gaatttcttt ctgcaaatct gttcaggcgt tttctcaggt aatccgacat gggaaaggag
    25561 tctgaaggca gtacgagata tagtgcttta tcctgcttga ttccaattac atattcattg
    25621 ccgtctttaa gaaaaaacgc ttcctgttgc tttttaaaat ttgttttaaa attccattca
    25681 aataaagaag caatgattgc aagtgcaaga atgggataaa gtgttttgat ttttttccga
    25741 tagaacatga tgtagaaaag agcaatgatt aaggccaata aaatggctgg ccatgcagac
    25801 aggtaaatgt tgctcatgac agatccgggc aatgattcaa tagtgataac aataaaattc
    25861 attgcttttg tcagccagat ggttccaaaa gcaacggcag tattgactaa tggaatccat
    25921 gagagcaata cggctgccat ccctgcatac agaaccagac tcgatagtgg aacaacaata
    25981 agatttgata ttaagaaata aaccggaaat tgtccgaaat aatacaggat aaatgggagt
    26041 gtgattagtt gtgctgctat cgaaacgcag gccagttgcc atattttatc gagccacttg
    26101 ttttggatgt gccatagatt atacagcggt cgttgaaata acagaattcc gagaacggcc
    26161 acaaaagaaa gctggaagcc gatttctcca agactgcccg gattgaaggc aagcagtaaa
    26221 aatgctgccg ccgccaaact attgtagggg ctgatattgc gttgcaatgc tgtgccaaaa
    26281 accacaaagc tgatcattac agcagagcgg agcaccgagg ccgaaagtcc ggtgagaaat
    26341 gcatagaacc agatgagcga aaggacgagt aaaacactta accactttct ttttttgaac
    26401 catccgaaaa ggcctacaaa tgcggtaatc atgatataaa ttacgccgac atgtaatcct
    26461 gaaaccgaca tgatatgtat gaccccggcc ttgctgtagg cgttcatcat gtcggcactg
    26521 atgtcggctc tgtaacctgt tagcatggct gccacaacgc ttgattcatc attgtctcca
    26581 agttgattct gaagaatttt caccattttg tcgcgtactg aaagtgcaaa tgtcatgatt
    26641 acatttcctt gttttttttc cagaatggtg taatggtcgt cgcggatata tgtctgatga
    26701 aaaatatttt tgtcggcaag atactttttg taattgaaat cggaattaaa tagggggttt
    26761 tgaactgtgt tcagataaca cttcaccgct atgatgtctc cgtatgaaag aacaggcgtg
    26821 gtatcaggat ttgaaatgta taacaatatt ttgccgctgc aacggatcca acccgaatcg
    26881 gttttcacag caagtgaagt tgctgtggtt ttcatgttgt tgtttttctg ctctacggta
    26941 ttgtctattt tgagtatgag ttctgcggcc tgattttccg gtagtttgcc gatgaagtca
    27001 cttttgtttc tatgatcgct gtagtgtgca ttcagccatc caaaagcaag aatacagcat
    27061 attatcaggg ctccctgaat cgttcttgtt ttatacgtgt ttaatgattt gaaaacaaaa
    27121 aaccatgctg ccaatcctgt tatcagcgcc agaaatacag gcaatatcca tgaatgtgca
    27181 acatcagccc atccacttcg gtaaataagc aaaccggcaa taaatggaat caatatcctg
    27241 acaaagggtg ctttggacca ggaattcata attaattatt ctgtttttga caatgaatca
    27301 atggttgtaa tttcggtacc aagatagtaa aacttcagaa tatcgatata tgaaaaacct
    27361 tgtttggcca tgtttatggc gccttcctgg ctcatcccaa ctccatgccc gaatcctttc
    27421 ccggtaatga tgatattgtt accatcttct ttaatattga aaaaagttga acgcagtttc
    27481 agatcgtcgc ggattttgcg caatccgata tttcctgtga gaaatacctg tcggtggatt
    27541 tgtgtgaaat gggttacttt aacaactgcg gctggatcgt ttacaggata attaaaattg
    27601 cgttgcaaat aatcaagcca ttcgttgcgc gtcatggttt tttgccagct gtagcttgtc
    27661 tgaccaatgc tgtacgggtc ttcaacggag cgtaaatatg gcaagggccg accccataca
    27721 tcctctgagt tgcaggtttg tccacccgaa ttggaatgaa aaaccgcaat gataggttgc
    27781 tttgacaagg tgtcaataat aatttcacca gctgttttcg aagtggccat cataatttct
    27841 gtattggcac agcggccttt atacacctga caacaggtct ggtcacacat ctgatatccg
    27901 tctgcagcat gtttggttat gtttttcaat gtatatgtgc gggctgcaac ggcctgaacc
    27961 ttataaaatt cgctgttttt tgcaatccct gcctcggatt gaacaacgcc ggcaacatag
    28021 tgttcaagct ctacttcatt aaacagccac atagttttat ctacccgcag cgaaatgctc
    28081 aaatcatcgt cgtagagccg gattgattta cctgctttta gttgaaaaat attcagaaat
    28141 ccaacacccc gtaatttgaa atgtttactg caaaaaattg tgtcggtttt ggtaagtata
    28201 accaggtcgt agtgcaaagg ggcaatatga agtgaatctc ctttgttgaa tagtttcact
    28261 gtttggcctg ctgtgtcctg taactgatag gttcccgaaa aaatgacaat gtcggttgaa
    28321 tgtatggttt tatcagcgtg gatgcgaact tttacatcct gttgtgccac tgataactgg
    28381 gcatacagca ttgtgataca aaatgacaac aggattttaa tgttcatgtc agttgctgca
    28441 aattttgata atctgtttgg ccactcgctc cgatgctcct tttccgccaa gtctggcata
    28501 cagcaaatcg tattccgaaa gcatccgtgt tcgttcttcg ccaggcgcag caagtttttc
    28561 catttcatta accatgttat ctttattcaa gtcgcgctga atcagttcgc ggcaaacttc
    28621 tttacctgcg atcagattta ccagcgaaat gaattggatg tttttcccta ccaatctttt
    28681 tgcaatcatg tatgaaattg cattggcttt atagcagact acaagcggaa cacggaacaa
    28741 agccgtttca agggtggcag taccactcgt tacaaaggca caccgggcct ttgtaagcaa
    28801 tgcataggtc tgatctgtta ttatttcaat gttacgcccc tcagtgttgt atagctcgct
    28861 tggtaaaaga ctgactgccc cgattataat acggtgtgtc gatgaaaatg catctgaagc
    28921 ttcgagcatg agtggcagca tttttttcag ttcctgtttg cgactccccg gaagcagcgc
    28981 cagtattggc ttttcagaat cattccaatc tgtttgtgta ttattgtttt tgaaatcttc
    29041 gactgcatcg atcagcggat gaccttcgta gcgtacattc attccgtggt ttgaataaaa
    29101 ttccttttcg aatggaagaa tggtgaacat ttcattgact gtgcgacgga ttatttttac
    29161 acgtgatttt ttccaggccc agacttgagg cgaaatgtaa taaatgactt tgattttttt
    29221 ttcgtgcgcc cattttgcca tgcgcagatt gaatcccgga tagtcaatca gaataagcgc
    29281 atcgggttta aattctgaca cctgagcttt gcataatctg aaattgcgca tgatggtgcg
    29341 cagatttatc agcacttcta caaagcccat gaatgccagt tcccggatgt gtttcttgac
    29401 atcggcacct tgctgaatca tccgatcgcc tccccaggcc ttgatttcgg cattagtgtc
    29461 attcagcttg agatgcttaa taagattaga accatgcaga tcgccggaag cttcaccggc
    29521 tatgacaaaa tatttcatgc cgaccagttg aaataaaaat aaaccatggc catgagaaaa
    29581 gtgatgagca ggatcccttt tcctgttttg tcggccttga gtttaaccag ataatatctg
    29641 aacggaatca ggttggcgca tattgatagc aactggtcag tgcttttttg aaacaagtct
    29701 ccggtgcttc ctgcaaaaat cagggtaatg ccctgaatta taaaccatgt taaaaccggc
    29761 aacacgattg caattccaag tccaagcaag aaattgtctc ttcgaaaaaa ttctttcata
    29821 atccctgaaa ttgatttctg gtaatatcga gaatggtgtg agcggtaagg tcaatggtaa
    29881 ccggaacgat gctgatgaat ccttgtttca atgcgttttc atcagtgtct tcctgtactt
    29941 ccggaataat aaaatctcct ttgagccagt aatatggttg atgatgcaca tcgagacgtg
    30001 aatcaaattc ttcgttccag taaccgatgc tttgtttgca gactttcaca cctgcaggtt
    30061 tatcggtgtc cggaaaattg acgttgatgc aggtccccgc aggaagtccg ttcttcatca
    30121 gtcgtgcact tatttcctga acatatgatt ccatgaaact gaaatcgatt ttcggatttg
    30181 gattacacat tgaaaatcct gctgagggaa tattctgcat ggcgccttcg atggccgcag
    30241 ccatggttcc actgtacagt acattgatgg aatgattgcc tccgtgattg attccactga
    30301 caatcagatc gggtttttgt ttcagaatct gattaaggcc cagtttcacg cagtcggctg
    30361 gtgtgcctgt agtggtgtat ctttcaacat ctccgtcgtt tggcaaatgt tttatccgca
    30421 gtgggttttt gatcgttact gcatgtccca tacccgacat cccggtttcg ggtgccacaa
    30481 tcacaagctt tccaagaggc cgtagaaaag aaatgaggaa tttaattccc gctgcgtcta
    30541 ttccgtcgtc gtttgtaatt aaaatcgttt tattcataca ggtttagtcc acttcactca
    30601 ggtttttttc aagctccttc aatgctttat tctggtcttc gagattctga ttgccattca
    30661 caatgtccga tttgtttttt tcgatgtctt tttcggcatc ttcaattctt tttttgtaat
    30721 cttcgatatc ttttgcaagt ttttcattgt ctttggtaag accttcaata tcttttgttg
    30781 tcttggcaat tacatctttt tgttcgtcaa tttttttctg aacagcgcat ttggtttgtt
    30841 caacagcaaa gtcgtaaatc attttttctg caatcttgta acccgaagca tgttctttgg
    30901 aattcagata ggcgccaccc agattgaatg cgactttgaa aataacatgg cctccctttt
    30961 cttcaatcac tgagtaaata tcaatcgtat ttggcgatat tgatggcaat gttgcgtcgt
    31021 caacaaacat ttcttttttt ccggaaactt ttccgccggt atctttcatt ttatctttcc
    31081 atgccttggc aacatcatct tcactggcgc cggtaatggt cactaccaga caattgtttt
    31141 ttccgtcgcc gattttttca atgctttcgc tgacctttgt tttttgtgcc tgtgcaggga
    31201 ataaaatgac tgcagcagtt aaaatcaata agaatcgttt cataatgact atttttttgt
    31261 aaaagtacaa atttcgcctg aaatgagaaa atgagctgta tccttttgca tttttccgca
    31321 catcttttaa cttattctgt tacacgttac atgttacgcg ttacgcgtta cgatcgttac
    31381 agacgttaca tttcagcttt cagtcatcag ttttcagcac tgagaagaag cattgaactt
    31441 catttggcga tgatcgcgtg aaacgcgata aatgcgaaat tgtgattatt tggcaatcaa
    31501 aattcgcata atcgcataga cgccccatcg ccaaattcag agcgagtaag aatcttagcc
    31561 atgccccatg ttctatgcgt tgtgcattgt gcgttgagct gcaagccgca tgcttttgta
    31621 tatttgtaga aaattattcc gatggcactg atcaaaaaag tgaaaggcat attacctgaa
    31681 ttcggcactg attgcgtgat tgctgaaaat gcgaccatca cgggcgatgt agtcatgggc
    31741 gaccactgca gtgtatggtt caatgcagtc atccgcggcg atgtgcattc aatccgcatt
    31801 ggcaataatg taaatatcca ggataatgtg gttgttcact gtacctatca gaaatgtccg
    31861 gtctcgatcg gaaacaacgt ctccattgca catggcgcga tagttcatgg ctgcacgatt
    31921 cacgacaatg tcctgattgg aatgggttcc atcgtaatgg atgatgttgt tgtggaaagc
    31981 aactgcatca ttgcggccgg agcggttgtt accaagggaa cccgcattga aagcggctct
    32041 gtgtatgccg gcatgccggc aaaaaaaatc aaatcaattg ataagtcgct cctcgaaggt
    32101 gaagtgaagc ggatttcgga caactacatg atgtatgccg gctggtatga tgaagggctg
    32161 tagattttag aatatcgaac ggtgattttt gatttaagat ctgaattggg atttgggatg
    32221 tgatttttag atttacttga attctttttt gagtcgaaat attagacaga gatatcgaaa
    32281 cgacaataaa gattttgtat tgcaaccacc gattccgtgt ccgctccttt tcgcaaatta
    32341 atgtcatgta ccgcctgcag cgggataaac tcccgacaga atgttgtgtg tttcccgaca
    32401 ttctaccgtc agcagttgtg gcttgagtct cagtattgct gacgtggaat cgtcgggaat
    32461 atttttcaat atctccgagg tctgtgaaaa tatcggcatt cagtttattt cgtggagttg
    32521 acctgtatgt attgttctgt aagtcttcat actgatgtcg tcgggtattg ctctggattc
    32581 taaatttcaa agacagcaag ttttgagcaa tcactccacc aacactccca tcccacctct
    32641 caaatcctca atctacaaag aatttccccg gacgtcattt ccattttcga aaacgcaaac
    32701 cacttcttcc ctaaatcctt cagcgaagca ccgaaatgat aaacaacttc atcaataata
    32761 agaaagcggt cgtgcgattt tgaaaatacc agcaaatcaa tgggtgcata ttgttggttg
    32821 tgtttctgca aatcgagttt caattgtttt gtaatttgcg aagaataaat aatagcggaa
    32881 acattctttt ttcgcttgga aagtaatgtc agaacagttt catcaacata attgtcaatc
    32941 agaacaatgc tctgttttgc cgattttatc agttcagaca caaaagtgta tgcatcgaaa
    33001 atttgtccgt cgaaaaaaat gccttcattt ggaatcgttt ttttgccttc caaagcctta
    33061 aaaacctgct caaattttcg atcggtttca tgttgcttcg tctctatttt attaaaacga
    33121 tgctcgatca atgaattgtt taataccaac tgcctcattt tgataaaagc attgataatt
    33181 tgaatgctga ctgaaatggc cacctcgctg tttagaatag ccgacagcat ggcgactccc
    33241 tgctcagtaa aaacataagg aattgttctg cgttttgcgg tcgcattttg cgaccacaaa
    33301 gtttcattgg gttttgcggt cgcattttgc gacttcaaat tttcccattc atctggcgaa
    33361 agctgaaaca tgaaattctc cggaaacctt tttatgtttc gctttacctg ctcattgagt
    33421 cgttttgttt caacaccaaa tagatcagac aaatgaaagt ctaacattac ctgaacaccg
    33481 cgaatgctga aaattctatc ttcaggcgct gtttgctgtt tgataatact tttgctcata
    33541 taagaatttg agtgaatgaa gaatatcctt tttcccgctt ggaaaggatg gcattaagat
    33601 taaatattct atttctcttt tcacttccct tcctgcttca atttgaactg ttgcattttt
    33661 tgcaacagtt gcctccatgg aaagttctcc ttctttgtaa acatttcgta agtgccttga
    33721 aatgactgat ttatcccttt caaaaagttg cgcaatttga ttaagagaca accataccgt
    33781 atcctcatca accctcactt caatatgtga agcaagctca ttgggctggt atataatgat
    33841 ttcgtttttc atgactgaaa attattctct cttcctactt taaaggcgtc aaaaagagaa
    33901 tttaaaatta ctattatttt tgataagtac ttttatttta ccttccccaa acaccttaat
    33961 atttctttcc gatcgccgtg ccaccagtgc aggccgctgc cttcgcagat tgagtaatcc
    34021 ttgcattcgg cgcattgtcc ggttttggtc caactgcggt tacgcatgat ttcgaagcgg
    34081 ttgttccaga catcaaaaaa atcatcttta taaatgttgc cttgtgccag actgcggtcc
    34141 acattcgggc aggccgaaat gctgccgtcg gccagaacgg aggcgatgtg tatgcctgcc
    34201 cggcaaaaga aaaatccgtc gcgcactttc tcttcatatc tgcccaccca ggcctcgcag
    34261 ctgaacgatg ttttcatgcg tttttcctta cgcttctcaa taatgaattc catcagaaat
    34321 ttcaattgtt catcggtgag cgaaaggtct ttgtcgtttg ctgcgcgccc gattggcgtt
    34381 atggtaaaca ggcgccatga cttgatcccg gtctgcaaca gtgtatgatg aatttcatcg
    34441 agttcgctga tatttctctg gtttacacaa gtgaccacat ctgcgtttaa ccgtggactt
    34501 gacgaaatga gttcaatggc attcagtgct gcatcgtagc tgtattgcga attacgcatc
    34561 cattcatgtg ttgcagccat gccgtcaaga ctgatggtga ctgatcccat gccggcattc
    34621 agcagtttgt tgtgaatgtt attattgtac agaacgccgt ttgagacaat gccccagcgg
    34681 aatccgtttt gtctgagctg catgccgcac tctgcaagat cgttccggag cagcggttca
    34741 ccgcctgtca tgattacaat gatggagtct tttttatatt gtttttgcag aggtttgatt
    34801 gcattcaaaa aatcggagaa aggcatatcg ggcaactggc tgctgacact gcaatcgctt
    34861 ccgcaatggc ggcagttgag attgcatcta agggtgcatt cccagaacaa ataggataat
    34921 tcatgcaggg cagcttcgtt gtgtcggaag cgaagatgat ttctttgttt gaattttttg
    34981 aacaattgac tgcgtcttag tttgccgatg tcatgcgaat gacgatgcca ttttcaggaa
    35041 tgctgctcag aacggtatcc tgttccagaa aaaagccgtt tgcagctgta tcagcatcag
    35101 aaaacagcaa atggtattgt gtggccgatg gcacaaagaa gctgaaagcg ccgcttgagt
    35161 cggataaggc gaactgactg tcgctgttca caatcacttt aataccggaa acaggttggt
    35221 tggtggtgtc agaaagaatg gttcctttaa cgagtacatc atcgaattga tcctgaggcg
    35281 caccataaca ggcctgaaaa acaaacaatg ctgtcgagaa gctcaggaat ccgaaaagtt
    35341 tgcggcgaaa atttttgaac cagctggtca tggcgggtag aattaatttg ttagtataac
    35401 cagaagtaaa attacaaaat agtttgaatt gttgcttgtt ttaataaaac agtatctgcg
    35461 ttttggcaaa aaccacggcc tgtttatcca ataaaacatt gaattcatgg gggattgcca
    35521 gtaagccgat atttttatca acaaacttgt ctgctatgaa aatttgtgta aatttgccct
    35581 caacatctat tgtattatgg ctgaaatgac aacaggaaaa attgcccaga ttatcggacc
    35641 ggtaatcgat gtggttttcg aacgtgaaga agatcttccg aatatttaca atgcgcttac
    35701 ggttgaacgt gaaaatggcg aaacgctggt gcttgaatgt cagcaggaca ttggagaaaa
    35761 tacagtacgc gccattgcta tggatgctac cgatggcttg tcgcgcggaa tgcaagtagt
    35821 gaataccgga aagcccattg caatgcccgt tggagatcag atcaacggtc gtcttttcaa
    35881 cgtgattggt gaaccgattg atggtttgcc agcggtaaac agagataaaa cgtatgaaat
    35941 tcaccgtgaa ccaccgaaat ttgaaaatct cagtactgca tccgaggttt tatacaccgg
    36001 aattaaggtc attgacctga ttgaacctta ctcaaaagga ggaaaaattg gtctttttgg
    36061 tggtgccggc gtaggtaaaa cagtcattat tcaggaactt attaataata ttgccaagca
    36121 gtacagcggt ttttcggtat ttgccggagt gggtgagcgt acccgtgaag gtaatgacct
    36181 gttgcgtgaa atgatcgaag ctggtcttgt caattatggt gacgaattca agcacagcat
    36241 ggaacagggc ggctgggatc tgtcgaaagt ggatgttgaa aaattgaaga gcagcttgct
    36301 gtctatggtg ttcggacaga tgaacgaacc tcccggagcc cgtgcacgtg tggctttgag
    36361 cggtttgacc gttgccgaat atttccgtga tggtgatgaa aaatcaggtg gacgcgacat
    36421 tctgttcttc attgacaata ttttccgttt tactcaggcc ggttccgaag tgtctgctct
    36481 gttgggacgt atgccctctg ctgtgggtta tcagccgact ctggccaccg aaatgggtat
    36541 tatgcaggaa cgtatcactt caaccaaacg tggctccatc acttcagtac aagctgtgta
    36601 tgtgccagcc gatgacctta ccgacccggc gcctgcaacc acctttgctc accttgatgc
    36661 cactacggtt cttaatcgta aaatttctga acttggcatt tatccggccg tggatccgct
    36721 ggattcaact tcccgtattc ttgatccgaa cattgttggc gaagatcatt ataatactgc
    36781 ccagcgcgtg aaaaatattc tgcaacgcct caaggagctt caggatatta ttgccattct
    36841 gggtatggac gaactttcgg aagaagataa attggtggtt caccgtgcac gccgtgtaca
    36901 acgcttcctg tcacagcctt tctatgttgc aacccaattc accggaattc cgggtgtatt
    36961 tgtcagcatt gaagatacca tcaaaggttt caaaatgatt ctggatggcg aagtggatga
    37021 atatcccgaa gccgcattca acctcgttgg aaccattgaa gaagctatcg aaaaaggtcg
    37081 taaactgctt gaacaatcga aataacctgc cctatgcgca ttgaaattgt cagtcctgaa
    37141 aagcagctct atgcgggcga agtcagcatg gctcagtttc ccggaaccga gggctcgttt
    37201 caggtgttga ataaccacgc acccatgatt gctacaattg gagctggtgc cattcgtgtc
    37261 cgcaatgaaa acggaaatga agaaatggtg gaagtcaatg gcggtattgt tgaggttcag
    37321 aaaaataaaa ttctggttct ggctgaataa cgcttctttt tattgcagga tatccccaaa
    37381 agttcatttt gtgcattttt tgtatatttg cctgtgaaaa atattaaaat atgatacgga
    37441 atcttctttt tgtcgttatg atttttgcat cctgcgccgc tttttcacaa gagcaggtgg
    37501 tcgatgatat tcagattaat tctgatggta aaacacttac tatcaaaatc gatgatccta
    37561 aagcggttaa atatgatttg ttaatctaca aatcatatga agatatcaaa atcaatgatt
    37621 acggcgtcac aaaaaatccg gttactattg atatcagtac ctgggaacct gccatctatc
    37681 atattaaaat tgattatgca cgtattacgc agtttcgtca ctatgaagtg ctgaaagaag
    37741 aataataact tactgctgag ctcctcattg ggagcttttt ttatcttgaa accacaaatt
    37801 gaatggttgc aatttgccca tccagcatgg tttgcagaaa atacatgccg gctctcagtt
    37861 gtctgatgtc aatggtttta tccattgagg gaaccagcat tatttcttta ccctcaattg
    37921 tatatatgtg aatttcgctt tccggcttgt ttgaaaggat gatgtagtcg tcggccgggt
    37981 tagggaatac gctaaatgca attgcttcct gttcattcat ttgtgttgtg gggctgaatg
    38041 taatgaatgc cgaagggttt gaagctgaga gagtaatact ttgagaaatg gaagacaccc
    38101 catatttttc gcttacaagg ataaaactgc caaaaggaat tcctgaaaaa ctaaaatatc
    38161 cgtccgttcc gcaatgacga aaggaatagg gggtcatatt gttggaataa agcaatacat
    38221 catatcccga tgggtcatcg gaattgataa ttccgctgat ttcaccaatg ccggtcccgg
    38281 tttgttgggc aggtaaggta atgttggcgc agtaatcatc tgatgtcaga ttgagatagc
    38341 ttgctgtttc ccattgtagc actgagccaa ggtaggtggg aacagaattg ctgtgtagat
    38401 tcccgtctga ttcaatgata cgaactgaat agttgcagcg gggcaatgca gtaaaataat
    38461 aatagccata ttgctgataa cttgccgtat caataataaa ataatcattg ttgcttcgcc
    38521 caagaaggac agcagtacca taggttgtat tgttcccatc aagcaagacg ttcccgccaa
    38581 gaatatagga tgcagcagca ggaatgtcga aattttcgtg aagttgaatg gagtcaacaa
    38641 caaatccggt aatggaatcg gtgaatgtct gtgttagttt aactatgttt tgttcattga
    38701 tgaaaaaagc atcaatggaa ttacctgaac cctggtaaat atcgttgatt gtccattgat
    38761 agctttcgtg cagataactg cgtttcagac tatcgctggc atatattcgg gcaaaagagg
    38821 gacaagtgtt gtcggcaggt tgcgaaacca tcatatctgc aggcagctga atggatcctg
    38881 tacaaacaga tatattcaca tcttctccta tcacattgtt gtaattaaag gtcagcagat
    38941 atccttttcg ctgaaaatcg caatcgtatg tataaaccat tacatcgagg gggataatgg
    39001 catttgggat attgggagcg cttagactga aactaccgtt gcttgaggtt atcaactcat
    39061 tatgcagatt ttggacatat ggctgagccg atgattcaaa caccacaaat actttttggc
    39121 cgggaacagg ggcattgttt tctgctgaaa atatgcgtcc ttctagacca attcctttct
    39181 gagcggaaac aaatccggaa aaaagtacaa acaataatgg cagcagataa aacttcatca
    39241 ataggatagt ttatagatga gacgaagttt tagaccataa ctgaccggag tttttcctga
    39301 gagttcactg tcggcataca atggactcag acagtaacgg taagtagatt ctgcaccgaa
    39361 agagaatctt gagtttgtat tataaacaat gccgcctgcc agtgtgaatg accagaaatc
    39421 cttgtttctt gacagtgatt gattttgcca tgataatggc tgtatccctg atgcttcgct
    39481 gaattctgcc tgatttaatt cagatgtttt cagcaaaaaa gtataggaaa aaccgccctg
    39541 agcatacaac gataagcgat ggatgtcttt ttggaaaccc aatgcaagcg gaatttcgat
    39601 gtattgatag tgattgtttg cattgatgga atagttcgca gctacggaat catacatcgg
    39661 ataggtctcg gttgaaaatt gtggaatatc aaccagattt ccggcggtgt catatccctg
    39721 aacaaaccat attgaatcga caagcgtgta aacgccggtt tgtgcaaatt cattcagcat
    39781 gtaatcatat cggttgcgtt gataaaaatt caatgcattt acaccggtgc ggagataaaa
    39841 atctgaaaaa tgaaatgaaa tgtcaatgcc attatttata gccagcgaag ttatacgctc
    39901 attattttgt gtgccaaaat caaaagcgtc tggtccgctc tggtagctga ggtcgaacca
    39961 tgcaggaata atgtttttct cttgaaaatc cagattgggt ttgcttagga attgtggctg
    40021 ccattcaatg tgaggagcag aaatgggctg agttggcagc atggataaca ataccggtaa
    40081 ttctggagtt ctttctgttt ctgtttcaac tgtattcaga gccgggtttt cgtgcaatac
    40141 tgtttcaact gattctgcgc tgttttgcgg aataacaaac ggaatgaaat ccacctgttt
    40201 ttgttcagat ggtacaggta tattgctgat ggctgttagc atgggcaaac gttcaactga
    40261 aatattggtt gcgggtatat tttgtgcagc cggcaaattg tttgaatgct cagaatccga
    40321 tgagggattg gacactgtga tcattattat agtagcaata gttgaaagag accatacaac
    40381 aggtctgata agattggaga aaacagctgt tgacactgta gtggtaatgg caatacctgc
    40441 agcaaccttt ttccacgacc tttcaggtgg ttctacccgc aggtcgtcaa acttttctgc
    40501 gaaaagccgg tccagatcat tttcatgcaa tctgttcata gcacacggca tttatcgttt
    40561 ttagcttgtc ctgtaatact ttgcgggctc ttgaaagttg agatttcgaa gtgttttcgc
    40621 ttattcccaa cgagtcggca atttcccggt ggctgtaacc ttcaataaca tagaggttaa
    40681 acaccatccg gtaaccttcc ggaagctttt gaatgagtgt aagtaaggtt ttcaggctga
    40741 tattgtctgt tgcctcaacc gaaacactgt ctctgtttga gcaatcatcg atatcgaggg
    40801 tctgaagata tttgaggttc gacttgtagt agtttattgc agtatttacc atgatacgcc
    40861 ggacccaacc ttcgagcgag ccgtcgcatc tgaaattttt cagattctgg aatactttga
    40921 taaacccttc ctgcataatg tcttcggcct ccattttgtt tttggcatag cgcaaacaaa
    40981 caccaaacat cttcgaggcg tatcgtctgt aaagcaactc ctgcgattcg gctttaccgg
    41041 caagacaatt ctttaccagt tgggcttctc ccagggaacg gtcggtgttt gtttgttgaa
    41101 tatcagacat tagttgacgc cgatgggttg catgaatgtg gattattcct gtaatgtaaa
    41161 agtataataa atttgaatat aaaaagcact atatgtcaat aatcaataag ttaaaggttg
    41221 aatttttttt gttaacgtaa ttgaaaaaag ttggtgggaa agattatttt ggatgtaaaa
    41281 tattgttatt caacaacttc taatttgtat cgatatccaa tatcaaattg tttgcctttt
    41341 cctcccagtt tgttgattcc ctgaatgatg aaagaaatgc cgaaataatc gagtacgaga
    41401 cctccgggaa cacaatacaa tgatttgatt gaaggtggca acagtcctgc tgccagacag
    41461 gcaaatccgc caacggtgag tgctgctccg ccaagaacca tacctgcacc ggccagtttg
    41521 aacatggctg attttggttt acgatctaca ataatctgta tctgatggat aggcattgta
    41581 actgaatcta caacaagatt cgatttgtct atacttttaa tcttgccgca aatctcgctg
    41641 gtgtcgccga aataaacaat tacccggtct ccggctctga attccgttgt tttttccggt
    41701 ttgtcgatat gcgtaagttt cagaaaacag gattgttggg caagaagttg cataaaaatc
    41761 aaggtgcatg ccaaggtggt aaagagtttc atccgtcagg ttttttgcaa agctaaaaaa
    41821 aactctgttt ataccgtgta aatgttgact tgattgcttt gttttggcat tcaatgccct
    41881 gttgataaaa atcagcttgt ttgaatcagt ggtattagtt atctttgctc aaaatttcaa
    41941 cacttgatca aatttcgacg aagcagagct gaacgatatg caaatccggc ccaatggacg
    42001 gatttcaaca gctttatacg tcgaaacaga cgtaggttct tgagtcagca aagaccattg
    42061 gcaatatggt ttaccggatt gtcgggctcg ggtaaaacaa ccctttcgga tagtttaaac
    42121 agcataattc ttcgaaaagg ttatttcact aaggtttttg atggcgatgt tattcgtacc
    42181 ggtctttgtt cagatcttgg cttcagcgag gatgaccgtc gcgaaaatat ccgccgcacg
    42241 gccgaggttg ccagaatgtt tatggattcg ggcttgattg ttttgtgtag ttttattagt
    42301 cctacacacg aaatccgtga attggcacga cgtattgttg gcccggaccg ttttgtggag
    42361 gtctatgtca attgtccaat cgaagtttgc gagctccgtg atgtcaaagg tctctataaa
    42421 aaatacaggt tgggtttact taaaaatatg accggatttg actctccgta tgaagctccg
    42481 ctgaatcccg atattgaaat cagaaccgat ctctgggatg ttaacaaatg ctctcgttac
    42541 ctgtttcgtc aggttataaa acacgttaaa tacagaaaga aaaaatgaaa tacagtttga
    42601 atcaccttca ggaactggag gcagagtcca ttttcatcat gcgcgaggtg gcagcccagt
    42661 tcgaaaatcc gggtttgctt ttctctggtg gaaaagattc gattgtgatg gttcatattg
    42721 cccgcaaggc attctggcct gcaaaaatcc cttttccgct tgtgcacatt gataccggac
    42781 ataattttga ggaaacattg caattccgcg atgaattggt tgcggaactt ggcgcacggc
    42841 tggttgttgg aagtgttcag gagtcgattg acaaaggaag ggcagtggaa gagaaaggtc
    42901 ctggttccag caggaatatt ttgcaaaccg ttactttgct cgataccatt gaagtaaata
    42961 aattcgatgc aatgctgggt ggtggccgcc gtgatgagga aaaagcccgt gccaaagaac
    43021 gttttttcag ccatagagat gaatttggac agtgggatcc taaaaaccaa cgtcctgaat
    43081 tgtggaatct tttcaatggc agaaaaaaaa tgggcgagca tttcagggtt tttcccctgt
    43141 cgaactggac tgaattggat gtctggatgt atatccgacg tgagaatata aaaattccaa
    43201 atctatattt cagccatgac cggccagtat tcaaacgcga tggaatgtgg cttgcatggg
    43261 ctccctttat gacaatgaag gacgatgaga ttcttgaaac gaaaactgtt cgatgccgca
    43321 ctatcggcga tatgacatgt accggtgttg tgctttccga ggccaattgc atggacgaaa
    43381 ttattgccga agtggctgca tcgagacaaa ccgaacgtgg caaccgggcc gacgataaac
    43441 gttcggaaag tgctatggaa gacagaaaaa aagaaggtta tttttaattg acagtcatga
    43501 acgaagaatt taacagcgac gagtattttg atatggaatt gcttcgcttt accaccgccg
    43561 gcagtgttga cgatggcaaa agtaccctga tcggacgctt attgtatgat tcaaaagcca
    43621 tctttgaaga tcagtatgaa gcagtgaaat cggcttctga gaaacggggt gaggagtata
    43681 tcaacctcgc tttactgacc gatggcttgc gggctgaacg cgaacaaggc attaccattg
    43741 atgtggcata tcgttatttc agcactccaa aacggaaatt tattattgct gatactcctg
    43801 gccatatcca atacacccgc aatatggtta ccggtgcatc aactgccaat ctggcaatta
    43861 ttcttgttga tgcacgcaaa ggaattcttg aacaaacccg ccggcatgct tttattgcat
    43921 ctttgctgcg aattcctcat gttgtctttt gcatcaacaa aatggatctg gttgattatt
    43981 ccaaagaggt gtatgataaa atcctcgaag actttaaaca tttcagctcc aaactggagg
    44041 tcaacgacat tcgttttgtg cctatttcgg ctttgaaagg tgataatatt gttgaccgtt
    44101 caaccaatat gaattggtat caggggccaa cgctgcttta tctgctcgag accattcaca
    44161 ttgcatcgga ttatgatatg ataaattgtc gttttcctgt gcaatatgtc atcagacctt
    44221 tttccgacga gcatcatgat tacaggggct atgcaggccg catttccggt ggtattttta
    44281 aaccgggtga taaaataatg gctctccctt cgggttttac gacgaccatt aaacgtattg
    44341 atactttcga tggtccggta gaggaagctt tcccgcctca gtcggttaca ttgttgctcg
    44401 aagatgaaat tgacatcagc cggggggata tgctggtgcg cgaaaacaat gtgccgcaag
    44461 tgggacagga cattgatatc atgcttgtct ggatgaatga aaaagcattg aatgtgggtg
    44521 gcaaatacgg gcttaaacat acttcgaaag atttgcgttg ccttgtgaag gaagtgaaat
    44581 ataaagtgga catcaacact cttcaccgca atatggagga taaaaccgtt ggattgaatg
    44641 aaattgcccg tatttcactg cgtacaactc aacctgtgtt ttttgataaa tatcgtttga
    44701 atcggataac tggaagtgtt attcttattg atgaaggcac caatgaaaca gtgggagccg
    44761 gaatgataat ttaggttgtt agttttatct ttcgttacgt gttacaatcg taacaaggta
    44821 gcagggtgat aacaattgtt atca
//
LOCUS       DAOPKE010000014        39970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1050_length_39970_cov_14.814030, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000014 DAOPKE010000000
VERSION     DAOPKE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_39970_cov_14.814030"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..443)
                     /locus_tag="PKY63_03350"
     tRNA            complement(371..443)
                     /locus_tag="PKY63_03350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Gly,seq:gcc)
     gene            575..1474
                     /locus_tag="PKY63_03355"
     CDS             575..1474
                     /locus_tag="PKY63_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPE99682.1"
                     /translation="MKPGKIHFIAMLIAASALLSAFVSGKTKPIKTPEGFVFIPMGTT
                     NIGTDTFSVQAFWISKTEVTNKEYREFLADLKASGDMDAYQKALPDTTQWRDKLAYNE
                     PYVELYFRHPAYNDYPVVNISREQAEMFCVWLTKKMREAYGENINDVRLPYREEWVMA
                     ARGHNPEAVYPWKGVTLFNEKEHKYACNFRNMGAESIHYNDSTGNMEVWGKYGSCGIA
                     GSINDAADITAPTLSYWPNDYGLYNMAGNVAEMVQQSGIVVGGSWKSTGYDVRIESTA
                     PYYGPRSDVGFRPVITYTGKPKN"
     gene            1510..2388
                     /locus_tag="PKY63_03360"
     CDS             1510..2388
                     /locus_tag="PKY63_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPE99683.1"
                     /translation="MKTNKVTWVVFLLTGFIVFSSFAPGKKKTVKTPDGYVFVPMGAC
                     IIDSNKVTIQAFFMSKTEVTNKQYRTFLADLKASGDMEAYKKALPDTTKWQMDGNSYN
                     QPMVELYFHHPAYDNYPVVNVSREGAEMYCQWLTKKYEAENGPQKCAFRLPQREEWIW
                     AARGGLKMIPYPWGGPYLRNSKGCYLCNFRPLNEKHITYNHEKQQYELVPHAAESFSE
                     DGAFQTIITGHYAPNDYGLYDMSGNVAEMVAQSDVVVGGSWMSPGADVRIESVAPYTG
                     PAPTVGFRPVMTYLGE"
     gene            2510..4735
                     /locus_tag="PKY63_03365"
     CDS             2510..4735
                     /locus_tag="PKY63_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPE99684.1"
                     /translation="MSRFLSFLFSLMAATAIFAQTSADFTFENSPTGNALPEHGFLWG
                     NNYSCTIQNIIKHEGNNALELVRNANASNKDFGCFVFAIPVNFTGKMAQFKAFMKTEN
                     VDNGIASLFVRIDGSSNNYYFTNSVSNLYGTNDWYQFNTPQFSLGKGAETMYVGVILN
                     GSGSLWADDFQLLIDGKDYLKAPKIKGADFKADQDTAFSKSSGIDIGISNRVQVDNLA
                     LLCKVWGFLKYYHPSIAKGNYNWDNELFRFLPTYLPITDKKQRNDSLLSWVNKLGPVK
                     GKGNKMKIKKNTKLTPELGWIADTTTLGSNLSAALVKIRNSKRGATHYYISLQDNVGN
                     PVFEHERSYRTISWNDDGFRLLTVFRYYNVIEYFFPNRHLIGEDWHNIPNEFILKMIA
                     AKDETEAQMVILEMITRINDTHAYPRFGEEFSNAFYGRNYANYKLTFIENKAVVSGYL
                     NESMVKDDDLQIGDIIIDINGETVMQKVDRLKKYVPASNEPQRLYQISRYLLRSSNRG
                     ITVTYVRDGKTASKSLPCHTPETIFAKTNSGAESATPTWRFLTPEIGYIFMGTINSKE
                     LTKMMGELRLTKGIVIDLRCYPSDFVVFSLSQYFNIKSTPFAKFSNGSIQNPGMFTFG
                     KPLSVPGMKTAYNGKIVILVNEKTISQAEYTAMALRSTPIATVVGSTTAGADGNISPI
                     TLPGGIVTNISGIGVYYPDGTETQRVGIVPDVVVTPTLRGFIEGRDEVLEKAVQIIEG
                     K"
     gene            4807..6303
                     /locus_tag="PKY63_03370"
     CDS             4807..6303
                     /locus_tag="PKY63_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99685.1"
                     /translation="MKKIIFSFLLCMLFITATKAQSVNGIVLADTANYKVIQVFGGHY
                     QRGFAYGYLCADNIMDIWENYIQPSYGMYMPFAEAIVGNPATFSVDSEYVIEARGIID
                     GVAYAGYDTTGLSYLDLFVVNFMTDLEGFMSVKGFPETQQCSSLMNWGAATQGTDLNG
                     KAVISHFLDALTLNQVISRNQVVVVHFPTETDEQPWLMTGTAGQMVASQALNNNGLVL
                     FLNTVNGFTAQQNKGYEPMTLTLRKAVEKNDFNGDGQTNVNDVKAAIDANTNGYARGF
                     IVSAIAPSTIGNDSLIAMVAECAPTVPYITYRNINDVDSVDGTNLYAANNFIKRNNAQ
                     DYCSRYNNVKDSINHYFHGVNIGSVDNKEIMYTQSTQSSCLQFIQVIPENGIFTMSVA
                     NNASPAYTQTPATLSYSYLFNYAGLQDLTTNNTIYIYPNPATDLIYMDVAPEENYSYY
                     ISDLSGKIISGGQLRHQNSICISALPAGLYFIQLVSSKKMLTGKFLKQ"
     gene            complement(6349..7104)
                     /locus_tag="PKY63_03375"
     CDS             complement(6349..7104)
                     /locus_tag="PKY63_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPE99686.1"
                     /translation="MFNKWKTLIVDDEKPARERLRRLLGKYDGRFEIIDEAADSFDAG
                     KKIEMLMPELVFLDIQMPGPNVFEMLTGLDHKPAIVFCTAYDSYAMEAFNTFSVDYLL
                     KPVEKDRLDMTLQKLERLNSSALPELYRTIENMVIQKPVPVTITHKLGNKIIPVKLEE
                     VVYFEANDKYVNFFNTAGTTYFTDQTLTLLSQKLPEKFIRISKSEIVNSDCILEIQKY
                     FRGRFVFVMNDKARTKLISGQVYGEEIRKRFEI"
     gene            complement(7113..9443)
                     /locus_tag="PKY63_03380"
     CDS             complement(7113..9443)
                     /locus_tag="PKY63_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPE99687.1"
                     /translation="MKTRILLFLSFLACSFFSEAAAGDTTWILTNSGNGIRVSIANDF
                     LHYYVPSVIKIETSNPSVPVNITVTGGVIEKLGGNRYLVNPMKLNGLKLKVNGTFFLL
                     DVSPVPEPDVYLSGDKRRWKNVISMEDFLNSDSLIIDSTFGVFQFVSAVVTYIRKNGD
                     LVDLSMQTAQFPITRIKEDVGFDNNKKIWIEKIMLRAPDGTIKILQKEVSFRIINSYE
                     NEFVKQVVSFSSNSTDLNLMNTDLRIGIEGLAAERDIKTVNDIASELNEILKTIQVKV
                     TDYQPNLRIIFDTTFSDSVLINKGFTTTSAGYIARNRNIFFPFHKRMVMYVNPNTWGY
                     SVADLKRSIVEQLGNFKVSYHSFGKDSSIFDGGKNMSGLDKYMLSLLYSEGGEYKVKQ
                     MLNKNEEGESKTEVFIITLILSICLFFLFSELYKYYGLDIRLKKWRFGIARKLLEALL
                     LAQIPVLAVLILLSEKFLAGDYHEANLLLFVEFIIFPFAVFCTLLFVAAEPLLKKINK
                     TWLAAVFNLILSFTVLFISYQLVYFFISPELISLSEISWQVLLVGFLIVLYRMYIRLQ
                     SQKIAGLLQQKELELSKQKELKFKSDLNALQARINPHFLYNSLNSIASLAHSNADKTE
                     KMALSLSKLFRYNLNKEDDMYATINDEAEMTATYLEIEKIRFEDKMIFSIDLQESLRQ
                     VKVPKFLLQPLAENAVKHGVSKLAGQGIIEIRIFEKNEKIVFEVADNGPEFPGGLMNG
                     YGLQNVYEKLKLLYKEPFDIEFVNSPKKKVVITLNK"
     gene            9620..10246
                     /locus_tag="PKY63_03385"
     CDS             9620..10246
                     /locus_tag="PKY63_03385"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPE99688.1"
                     /translation="MPRLLLLLSLRLAQCTAFSFFLLSSAQAQTDSVWYNSVIAWQQE
                     MNREYADSATSPLTPEDRAIFKGLPFYAPNADFCVNAVLEITPNAKPFIMKTSSAKTP
                     TYRQYGVFHFKIKGQSFSLPAYQRVEMLTSDEYADYLFLPFNDLTNGESTYISGRYMD
                     CRIQKDNLYILDFNKAYNPYCAYNHKYSCPIPPGENFLNIRIEAGVLK"
     gene            complement(10286..10861)
                     /locus_tag="PKY63_03390"
     CDS             complement(10286..10861)
                     /locus_tag="PKY63_03390"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPE99689.1"
                     /translation="MKNISVWASGNGSNAENLMQYFSSHSEIRIDHILCNNAEAGVIE
                     RARRLGVDCFVYSRKDFREGDVVLNKLLKRKTDYIVLSGFLQMVPAEIIKRFPEAIIN
                     IHPALLPDFGGKGMFGHYVHEAVIAAGKTQSGITIHLVDEIYDHGKILMQQFCPVFEN
                     DTPDMLAARVHELEYKWFPIAVEQYLKVVRS"
     gene            complement(10932..11696)
                     /locus_tag="PKY63_03395"
     CDS             complement(10932..11696)
                     /locus_tag="PKY63_03395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPE99690.1"
                     /translation="MKISLSVVIITFNEERNIGRCIDSVRDIADDIVVIDSFSEDRTK
                     EICLEKGVRFIEHAFEGHIQQKNFAITQAAYPHVLSLDADEAPNEILCDNIRKVKENF
                     EADGYTMNRLTNYCGHWVRHCGWYPDRKLRLWDSRKGQWTGLNPHDRYEMEPGSVIKH
                     LQGDLLHYSYYTREDHLKQIQKFTDILAKSMFDTGRRTSWPGIIAATVFKFIRDYFLK
                     LGILDGKAGFTISWLSAGASFKKYRKLLLLQNNIPQ"
     gene            complement(11943..12512)
                     /locus_tag="PKY63_03400"
     CDS             complement(11943..12512)
                     /locus_tag="PKY63_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99691.1"
                     /translation="MRRFCFKEGDSFISFGSGLYGLVNAVPRKHAVLQDFKSVGVPPL
                     FFRYEKAISDYIGLGGTVFFHLPAFMWNKTVDVYNNETWTYEPVVYAEKYSGLSLALL
                     GRMNFHFATTRNRDTYIGLGAGYEFYMMNFQSDDPLATSRVPNRPLPFSGELIFGYRN
                     YFSDNTAFYAEIGYGKMLLNIGLTIALGS"
     gene            12672..15092
                     /locus_tag="PKY63_03405"
     CDS             12672..15092
                     /locus_tag="PKY63_03405"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPE99692.1"
                     /translation="MKQQLLFLLFLTVVSVSLNAQQYSISGKVTDAETGNALPFVNLV
                     INSSPHGSISDIDGNFILKSNEPIRYIRASYVGYKPDTIETNGSPSVNFSLQKSSIQL
                     NEVIILPGENPAHRIIDSAIAHRKENDPFQLEAFAYQSYSKFLVTAEMDSLMSIPDEL
                     LDSAHLELKHFFSKQHVFLMENMAERKFLKPGNDNEILTASRVSGFGDPLLTLLMSQI
                     QSFSFYKTLITISDKNYVNPISSGSTDKYFFLLEDTIYDHSDTVFVISYRPRRNAHFD
                     ALKGLLYIHTAGWALQCVIAEPNTEEPGMSVRIKQNYSRPDSIHWFPSQLHTDILFSS
                     LKVYEYPVYGHGVTELKNIQINPALKKSDFAAAKVIIEPDAKLKDSLFWSLNRPQPLS
                     QKEINTYRLIDSIGRKAHFDRLLDITTTLLSGFIPVGPLAIDINALYRYNVAEGSRPG
                     LGLKTNYRVSKTWCLYGYYGYGLRDKEHKFASRIDINLKKAKDTKISFGFKQDIRETG
                     SMNNDLNTNGILSLEGLRNYMISRIDYFDQAHFDFHSRIGRSWTITSRVSRSAFSAPD
                     SVLYGNYNEYGFAGTRSFILSDINVFLVYSKGMEFIQSEDYSLPIGGNTSNPVITIDL
                     RQSLKNIAGGDFQRTSAMMRISQTLKSRYIGKFSWMLQGAVVSPDVPLSLAFNAPASY
                     RHFTVSSPNSFETMRMNEFFSTQCVFLFLRHSFESLLFGNRKFSPSPELVTNVAWGDY
                     KNTTHLNVPDFKTLSRGYFESGLMINRILDAGFYSLGVGCLYRYGPYRLPTLKENLTF
                     KLTLNYHL"
     gene            complement(15064..16185)
                     /locus_tag="PKY63_03410"
     CDS             complement(15064..16185)
                     /locus_tag="PKY63_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPE99693.1"
                     /translation="MFDVITRESVSDVRQQHVVLMNRYMPELLKNISPMVMVLTRNRQ
                     IVYVNKSMEKFLGVEDCSDLLGKRPGEAMHCEYADVSRSGCGASVFCKYCGFNQALFD
                     AEIGKPASTYDCHLLNKSGETYNLGVTITPFEHMANRYMFCVLENRVDTHYRDLLENI
                     FSTNLKSILSSMQSLVKASGHFSAAEFQPLMKAQLMQIDEAINNFEILQKIEKGEPLP
                     GHQEWFSVKQLVDEVITNLRLRQDLRLKYIKKAIPDMMIFSNRMLLGYVLSNLVKNAM
                     EAENDRSEILVSVIDSTATISFKIKNQSVMDERKQKLVFTRFAAGGDGKMGLGTYCAK
                     KLATNYLSGKISFISNREKGTVFTIRIPKLVRDDSSMSV"
     gene            complement(16318..17364)
                     /locus_tag="PKY63_03415"
     CDS             complement(16318..17364)
                     /locus_tag="PKY63_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HPE99694.1"
                     /translation="MINIENAYLSKLIIHRVGNKVLEDGLTLSDELVSLSEAYLSSLG
                     GPLLVPFGNEDLLYNFYHDTDVNLNVMFTRCKKIFRSPDEEFIEASVAAAKELYELMQ
                     NPKLHGGEFWVAYFKGIVVDDEETDAVAFYLMDERKNFVKLYNKETNYAIEMQSGTDP
                     SGFLSGCLICNTGLDEGFIVSATGKSKSLDLRMWMDVFMILKQNIDSFYRTQLAMRMC
                     KDFVMEKMPEEFETTRPDQAELLIKSMDFFKASDEFALKNFQEDVLKQPELKDSFTHF
                     AKQFSREQDLNMGENFLVNERAVKKMARHFKSVIKLDTNFHIYVHGRREWIERGFDEE
                     KKLNFYKTYFEKED"
     gene            complement(17457..18215)
                     /locus_tag="PKY63_03420"
     CDS             complement(17457..18215)
                     /locus_tag="PKY63_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPE99695.1"
                     /translation="MITNISKEGLALVSGYRITSADCDIYARLKPSALLGFLIQSAID
                     SADRMGFGFRDLKKQKLFWVLSRLTLEMKTIPQWYDEIEIETWPRDIEKILYRRDFLI
                     RQNGVITGRATSHWLAIDRQTKKPGKIDLEDEWMFISLRDKQAIEAAPEKIQSFCSKS
                     TDNVETCFTDFDLNGHVTSTRYLEWVMNALPADFQRQHVFEKFIVNYIRETLPGERIQ
                     TKYQWPELKTFNFEAQHQSSGNAAFRSVVVFREI"
     gene            complement(18371..20038)
                     /locus_tag="PKY63_03425"
     CDS             complement(18371..20038)
                     /locus_tag="PKY63_03425"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPE99696.1"
                     /translation="MTHTKQAWGTRVGLILAMAGNAVGFGNFLRFPVQAIQNGGGAFI
                     IPYLVSFLMLGLPLLFIEWSIGRYGGSHGFHSTPFMMQSMDRRRLWKYVGVFGIFSNI
                     GIASYYCYMESWTLAYIFHSVIGTFNGMDQHQVVQLFNDYVSIDKATLIPYEPIVFFL
                     ICLGINVFILSKGLSGGVEKVAKIGVPLLLVFGLLLAIRAVTLKAGTAGAINDSSVGL
                     NFLWTPDFDSIYNPKVWFAAASQIFFTLSLGMGSIQAYASYLRKKDDIALNAMSAGWM
                     NEFVEVVLGAAVIIPIAVGYLGVAKVQELAGLGGLGLGFKTIPYLFSQWGPLMATIAG
                     VLWFGLLFFAGITSSLAMGTPVQGFLEDEFKWSRKKTALAFGLIILVIGMPTVLFFNQ
                     GVFDEYDYWTGSFALFVFAAFEAVLFAWFFGIKKGWKEINTGADIKVSRVFRFLIAYV
                     TPVLLIIVFLGAVFTPVGNDWIAAFQGLSDGQGWAFDHESLVGKLMNNGLKEKIAVAT
                     DPAVIADLEYRLLLTNLSRLLLLTVFVGIALLVRKAYYRHKKEGRLK"
     gene            complement(20127..21425)
                     /locus_tag="PKY63_03430"
     CDS             complement(20127..21425)
                     /locus_tag="PKY63_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPE99697.1"
                     /translation="MNIPESFIHYVWEKGLAGQQFILDTGDDCRILKPGKRGNRDGPD
                     FENTEIVINQQHLAGNAEIHLCSSDWFRHGHHTDPAYDKTILHIVLKNDAIICNQDGC
                     LIPSIVLPADEITRLYENYSEKSMFGYGRLHCSDYISAADPERFLALAEKLAKERIIR
                     KSVRIQTLLENNHFDWPETTWKAIAWSFGLNQNMVPMEWLSNTISWKNLRRAGNVFSI
                     EAILLGQGGFLDKEISSENGYLLALKQEYLHRKNMFMLSPVDLNAWKYNPVRPSAMPE
                     SRLAQVAALVSLVPELFSELTQANTKKDFYQILRQPVSGYWKSHCRPETERKNGGDGE
                     IGKTSVDLILINAVVPLLIAFSEYTGNRSYENKAWFLLKSIAPEQNKIIDIFVRNAGI
                     KVCNALESQGLIELFDTFCSPGKCASCPVMNELIRSNNHE"
     gene            21565..21954
                     /locus_tag="PKY63_03435"
     CDS             21565..21954
                     /locus_tag="PKY63_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPE99698.1"
                     /translation="MRTLLLLIPFLALIMPLSISAQSEEPIYVVVDKMPEFPGGPNGL
                     GNFIRSNQSYPREALDAKIQGVVIASFIVEKDGKVSKPTIVSGIGHGCDEEVIRLIKM
                     MPVWKPGMKDGKEVRVKLNIPVEFKLG"
     gene            22071..22199
                     /locus_tag="PKY63_03440"
     CDS             22071..22199
                     /locus_tag="PKY63_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99699.1"
                     /translation="MKKLLLMLMGIVLSSFMASAQLNTEKRIEIDLRNGYSGETIF"
     gene            22203..23558
                     /locus_tag="PKY63_03445"
     CDS             22203..23558
                     /locus_tag="PKY63_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99700.1"
                     /translation="MHGYFILESKADDYVDGQAEIKYDLYSGDLELTETKSVLVPRKM
                     LFRELYYNDDYIYNMYATKGGEFMITSVEMKTMNIETATGSLPPKVILRDMKVLGNQA
                     YFLTSIKKAPAIYRVDLTSGKGTTIPITIGAFSPKKISSKNYQILENTKEILLFARAT
                     ISKATSELHMIRISEDDNVDKPIKISGVGDNAIISISGCRVDASKIIITGTYAKKGGL
                     SEGLFFGEVEEGTVNYIRYYNFLDLKDFLSYLPEKKQDKIEKKKAKKEAQGKEMTLDY
                     WIADHDVIVLEDGYLFLGEAYYPTYRTETYTTYVNGRPVTQYRTVFDGYQYTHATLAK
                     YTKDGEIMWDVCFEMNPGYKPFYVKRFIAISEQTATQISMVYTSGNEIVSKTMDFDGN
                     IISDEKWDFIETGNANEKTKRTFSNVDHWYGNFFLAYGSQVVKNGKEKRKVYFVNKIG
                     F"
     gene            23843..25036
                     /locus_tag="PKY63_03450"
     CDS             23843..25036
                     /locus_tag="PKY63_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99701.1"
                     /translation="MKEIYEISARRINAVSKEFSRGIINSVKWNNRLIGIRGARGIGK
                     TTLLLQHIHQSNYKPEEVLYVSLDNLWFSNHSLTDLADDFVKKGGMFLYIDEVHKYPE
                     WSATIKNLYDDYPDLKIVFTGSSLLEILNARADLSRRAVIYSMQGLSLREYLNMTLNK
                     HFEAYSLEEIVKNHVSIASKINKEIRPLKYFNTYLKHGYFPFFNEDLDVYYRRIGEVI
                     NMILEIELPMLRQLEVQYVPKIKQLLQTIAETAPFIPNVSKLSEKININRNTLASYLI
                     FLDEANLTNNLYRDSKGISRLQKPNKIFLENTNFMFALAPEEINIGNMRETFFFNQLS
                     HIHRIEFPEDGDFLIDRKFLFEIGGKTKSIRQIKGIANSYIAADQIEYGHNNIIPLWL
                     FGFLY"
     gene            complement(25112..26554)
                     /locus_tag="PKY63_03455"
     CDS             complement(25112..26554)
                     /locus_tag="PKY63_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE99702.1"
                     /translation="MKPYKKQNRFSLFSVNFQGIRRLGSLIGDAIAGKEYDYTSGRTA
                     KAIFLLSVPMVLEMLMESLFAIADIFFVSKLGSDAVSAVGLTESINTIVYAIGIGLAV
                     AATAIVSRRSGAKRYMAAAITSFQVLITSLMVSVPIATAGIIFSGDLLRMMGAEENIV
                     SNMSLYTAITLGTAPLIMLLFAANAVFRASGNPATAMKVLLVANGLNIVLDPILIFGL
                     GPIPAMGVEGAALATLIGRSVGVFMQLYILFNGTRLIRITRRAIMFHWKTIGEIFRLA
                     AGVTGQHLIGTASWIVLMRIMAEFGSVVVAGYTIAIRIVLFFLLPAEGFSNAAATMVG
                     QNLGAGKPHRAEQSAWSASYLNMAYLGTAGLLMALFPAFIIGLFINEPAVIEAGARGL
                     RMVSYGMAVYGLGSVMLNSINAAGDTLRPAWFMFAAFWIVEVPLAIWLSFNTSAVSNG
                     VYISILVAESFMALLALWWFRRGKWKTIEI"
     gene            complement(26541..26687)
                     /locus_tag="PKY63_03460"
     CDS             complement(26541..26687)
                     /locus_tag="PKY63_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99703.1"
                     /translation="MKTLYFEAVGLTITKAASAAIVRSMYVRKPGQDPGMQNHIKRRQ
                     YETI"
     gene            27344..28762
                     /gene="ahcY"
                     /locus_tag="PKY63_03465"
     CDS             27344..28762
                     /gene="ahcY"
                     /locus_tag="PKY63_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPE99704.1"
                     /translation="MSTLQTEATMNYKVKDIALADWGRKEIEIAEKEMPGLMALRKKY
                     GTEKPLKGARIMGSLHMTIQTAVLIETLKELGADVRWSSCNIFSTQDHAAAAIAASGT
                     PVFAWKGETLDEYWWTTSQALDFNGKGPHLVVDDGGDATLMIHKGFEIEKNPALLDTP
                     VTAADEKALYKMLKEIYVENPTRWTNLVKEWKGVSEETTTGVHRLYQMMERGELLVPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCAHSMRSY
                     GARVITTEIDPICALQAAMEGFEITTVEDALPEGNIYVTTTGNRDVITIEHMAKMKDE
                     SIVCNIGHFDNEIQVEQLENYPGIKKITIKPQVDKYIFPDGHCIYMLAEGRLVNLGCA
                     TGHPSFVMSNSFTNQTLAQIELWNNNYKTDVYRLPKRLDEEVARLHLGQLGVKLTQLS
                     QEQADYIGVPKEGPYKAEHYRY"
     gene            complement(28834..31116)
                     /locus_tag="PKY63_03470"
     CDS             complement(28834..31116)
                     /locus_tag="PKY63_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99705.1"
                     /translation="MIKHFYTIYFLFFAFLQIQAQSEFELRGDAEFKTGNFQAAWNYY
                     NEAARTDTSNIALWYKMGESSREFFNYNMAFQCYRIVYEKDLGKNFPQALFWMADLSR
                     NLSDYLMSKAYYSLYLEQTNNDAWFEEKAEEQLNYFDEIDKIRADTSLEVSVEHLSLP
                     VNSPFSEFGGFQQGEKKLYFTSVQPLLSEGENGLLGNSFLSSLYVSDFTSRGLSRPVK
                     VAEPLNNKVKHTANICFNEDASEVWFNRCEMSGEYKYRCDIYNAQLENGRWTYPQKIS
                     INNDQYTSTQPFFFVDSADNEVLYFVSDRPGGVGQLDIWFSVRKDGKYNVPTNLGSIV
                     NSPGNEITPFFDMESGRLYFSSDWYYGLGGYDVFYSAGSLSDWKKPVNAGIPICSPAN
                     DFYFNINSVDRDGYFTSNREGSYFFKGETCCNDLYAYYGQKKIKADTLPEPMDTLIVI
                     NEIKDRIEDLLPLDLYFHNDVPDPRSRDTVTLSTYDETYYSYMAMIERYKKEYAKGLS
                     ADDAYNAEAEIEVFFLAYVTNGFKKLLDLTPLMMEDLEAGSVVTMKVQGFCSPLTGTE
                     YNLYLAKRRISSIVNYFEKIDGGKIKPYINASASNGGRLIILAEPVGEALSDPFVSDN
                     PNDTRNSVYSISAARERRIQIVSYESDYAGQKTRKLSGPLLNLSSNDIKISLSGENNT
                     CIQITITNTGDEILEIISMKSAQPWLQAKLGGNVIIPGNVTTLDICVDKSILKNPALG
                     ILIINSNSRDKQSVVYVRVQ"
     gene            complement(31119..32132)
                     /locus_tag="PKY63_03475"
     CDS             complement(31119..32132)
                     /locus_tag="PKY63_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPE99706.1"
                     /translation="MRRIVLFAMLVCLSFFGTAQDIHFSQYSNVPFYLNPAKAGLMSE
                     QFRLSANTKLQWKSVTLPYQTYLAAGDMSLQRKGNHLGKVGIGVDFMREKAGDAAFGT
                     SGFGLSVSYIKALNRFNNRFFSAGVSFRMHQRSFNADQLTFDSQYNGLHYDPSLGGGE
                     TFSDFNFWYPVINAGGAYMMRMGSQDELVAGLSMLNINRPMQSHFNDNSIRLRTRWVA
                     DLMYKNAFTRNSFLCPSALFSKQGTFTELIIGADYRFVKSFDPWSFKAYSGGLFFRVG
                     DGFIFSARLDYLNYKFGISYDLNTSKLTPASRFRGGFEFSIVAVFNKADHKTTREIPC
                     PIF"
     gene            complement(32137..36111)
                     /locus_tag="PKY63_03480"
     CDS             complement(32137..36111)
                     /locus_tag="PKY63_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99707.1"
                     /translation="MRQLILSLCIILMASILSFAGVNHKDPDTLGGHIIFVENMGQWE
                     QSFAYKADLPTGQLFLYDNRLVVDLRDKAMVAELMRFKMLSPEEKKLRKQPDNIINAH
                     CYEMEFIGALYPEMRAESQIEPYYNYYLGNDKSRWKSNVHGFEKVKYKNLYQGIDLSV
                     GVENGSLKYEFAVSPNASVNDIRIKYNGVDNIRIENGNLIFGTSLGSVTELRPLAWQM
                     IDGDTVFVRCDYVLKNKTLGFEPGGNYNRNYELIIDPIVVFGTYSGSTADNWGYTATY
                     DQLGYVYAGGIVFGQGYPTTLGSYQVTYGGNVDVAVTKYNTDGTTLIYSTYLGGSSPD
                     VPNSIVVNSADELYIFATTGSADFPTTSGCIDSTFNGGSPYVLTYVINFTNGCDLGVT
                     CLNSSGTALIASTYIGGSLNDGLNMDAVLKHNYADDVRGEILLDANSNVFVVGSTASV
                     NFPVTYGVHQTMYGGGSQDGFILKCDRLLTTMIWSTYLGGSSADAIYSISLNELGKLS
                     VAGGTSSTNFPVSANAVMPVYQGGPSDGFVSLIQSDGSAVLRSSYWGTSAYDQVYFVE
                     TDKQNNVYLLGQSASTGTVLIQNAAWNTIGGGQFISKINPPLNTIIWSTRFGNGDGVV
                     NVSPTAFLVDYCHNIYLSAWGSPLLSGFGGTAGLPITANAFQTTTDNNDYYFLCIRDD
                     ASSIVFGSFYGGSSAEHVDGGTSRFDRMGRIYQSVCAGCGGYDDFPTTTGAWSNTNNS
                     TNCNNGVVKIDFSLPAIVADFNMPPVICLPYTVIFNNTSYFPNPGLATCFWDFGNGVT
                     STNCNPSYDYPVSGTYDVTLIVSDATACNSADTITKQITVLSSSTDTLAAVGICSGGM
                     TQIGILPFSDPTITCQWYPSNNLSNPAIANPTANPPITTQYYLVISNGVCFDTLYQTV
                     NVYDLIVDAGNDTSTCSPGITLTASFSGGTDFTVHWSSDANFSDTLNVYPSGSTCTTI
                     HTTPHTYYLQVSNGYCTETDEVFVDYQEMDLNVSITQPLCYGDSNGSVSLNPSGGVAP
                     FTYNWSTGDHTQNLNNLVSGLYVVTVYDVNGCVQSSNVMISQPQLLGYVSETGPVNCD
                     VACNGFVYGTTTGGTGPYVYQWSNGQSTEDLTGLCYGNYSLTITDSHDCKFQNTFDII
                     VDYIYDSVDVWTEQDTIYQGQSTTIHASSVPGVDYMWTPASGLADAYSSVTDASPTET
                     TTYTVVFDDGNGCIYSDTLRIVVLDVFCYDPYIFIPNSFTPNGDGNNDILYIRSRYIE
                     NMYFVIFDRWGEKVFETHDPLEGWDGTFRGQLLEPAVFDYYLEIDCFNKAQFIKKGNI
                     TLLR"
     gene            36229..37323
                     /locus_tag="PKY63_03485"
     CDS             36229..37323
                     /locus_tag="PKY63_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99708.1"
                     /translation="MKNILFVISGVFCLFSNPSISQIVEPTIITALPTCLHESSGIEA
                     ENTNSIWTHNDSGDSARIYQIDTLGNILRNICFQVDSAIDCEESTRDTNGNYYLGDFG
                     NNSNDRTNLRIYKITNPDSITTSTIIPELITFRFEDQELFPPDSAHMDFDCEAMFHFQ
                     DSLYLFSKNRSGNMFCKMYRLPDVAGDYIAELVDSFNTGTWITSADINAAGNKMVLLS
                     ESRLWLFSDIQGTNFFDGNAQMFNMYFSQKEAVVFVDDSTLYLTDEYLFGTGGNLYRI
                     DLNSVINGTDNQQKKQLTIFPNPVSNAFFINVSEINADFVVMVFDATGRTVQSCKSCS
                     EIDISSLCEGHYLVRVISENQCLSGRFEKF"
     gene            complement(37525..38604)
                     /locus_tag="PKY63_03490"
     CDS             complement(37525..38604)
                     /locus_tag="PKY63_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPE99709.1"
                     /translation="MKILDKYIVKRFLSSFVLAIALLLLIIIAFDVSEKIDEFIDRHA
                     TFRDILFKYYFNFIPYFINLFSPLFVFIAVVFVTSKMAGNTEIVAILTGGISFGRLLM
                     PFVFTGVFLAAFSFVLQNFLIPPANAVRLEFEYNFIRKNKPMGARNIHMQMAPGEYVY
                     MESFNFKSNRAMMFTLETIDFENGMSRKLSANMAIYDTISGQWQMTEVYDRRMKDSVE
                     KLRYLPTLDTSLAILPKDFNKDMENVDVMDFFELNDHIARQKMRGADNIKQLEVERHR
                     RIAFPFATVILTIIGVAVSSRKTRGGTGLHIGIGLALSFGFILFMQVSSTFATNGNLP
                     PMLAVWIPNIVFAGIAWLLVKWAPK"
     gene            complement(38604..39728)
                     /gene="tgt"
                     /locus_tag="PKY63_03495"
     CDS             complement(38604..39728)
                     /gene="tgt"
                     /locus_tag="PKY63_03495"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPE99710.1"
                     /translation="MNFSIQHTSGKARAGILTTDHGDILTPIFMPVGTLGAVKGVTFD
                     VLRNDIHAQIILGNTYHLYLRPGAGILQQAGGLHGFNGWDRPLLTDSGGYQVYSLSKI
                     RKITEDGVVFQSHIDGSRHQFTPENVMDMQRVIGADIMMAFDECTPYPADRKYAQKSM
                     QLTHQWLERCYNHFHKTKPLYGHNQGLFPIVQGSVYEDLREQSAKFISQFDSPGYAIG
                     GLSVGEPTETMYKITEQVCDILPQNKPRYLMGVGTPANIIRGISHGIDMFDCVMPTRN
                     GRNAMLFTRHGILNMRNKKWENDFSPIDECSPLEIDKTTTKSYLRHLFVAGEMLGPIL
                     ASIHNLAFYLRLVAEARQAVMEGNYEIWAAEAEKQTMTRL"
BASE COUNT        12480 a         8918 c         7639 g        10933 t
ORIGIN      
        1 agtcccgccc ggggtacatc taaaacacat aaacgcctga ttatcaatag taatcgggcg
       61 ttttcgtttc ccaatggggg caactttaca atattgattt tttgagactt aactgctgtc
      121 acactgagcc acgccaattt tatttgattt tggctcttgt cagtctgaga cctgcgttcc
      181 gcaggataaa ctccgtcgaa gcgtcggcag caaaaatcaa cagaaaagtc acgatagaaa
      241 aacgaaatcc cggcgcagta gccgggaact tcattggaga tttcagttga ttttctatat
      301 tctgcttgtt tttctcccca atgttcaact tagcaaacat aaaaaaagcc cccattactg
      361 gaggcctttg agcggaagac gagactcgga ctcgcgaccc cgaccttggc aaggtcgtgc
      421 tctaccaact gagctacttc cgcatttcgg actgcaaata tacaatcatt tttttatctg
      481 caaaaaaaaa tttccggaaa tttatatccc ctttgtaaaa tattccgacc tttactttcg
      541 tttacatttc tgttaaatga caaaaagcca acccatgaaa ccaggaaaaa ttcatttcat
      601 tgcaatgctg attgcggcat cagcgttgct ttcggccttt gtttcaggta aaaccaagcc
      661 aattaaaacg ccggaaggat tcgtttttat cccgatggga acaaccaaca ttggcacaga
      721 cactttttcg gtgcaggcct tctggatttc caaaacagaa gtcaccaaca aagagtaccg
      781 cgaatttctg gccgacctga aagccagcgg cgacatggac gcctatcaga aagctttgcc
      841 cgacactacc caatggagag acaaactggc ttacaacgag ccttatgtcg agctctactt
      901 ccgccatccg gcctacaatg attatccggt ggtgaacatc tcgcgcgaac aggccgaaat
      961 gttttgtgtg tggctgacga aaaaaatgcg cgaggcctat ggcgaaaaca ttaatgacgt
     1021 tcgcctgccc tatcgcgaag aatgggtgat ggccgcccgg ggacataatc ccgaagcagt
     1081 gtatccgtgg aagggagtca ctctttttaa tgaaaaagaa cacaagtatg cctgtaattt
     1141 cagaaacatg ggcgctgaaa gcatccatta taatgattct accggaaaca tggaagtctg
     1201 gggtaaatat ggcagctgtg gaatagctgg atcgatcaac gatgcagctg atataacagc
     1261 acccacattg tcgtactggc ccaacgatta cggattgtac aacatggcgg gaaatgtggc
     1321 cgaaatggtc cagcagagcg gcattgtagt agggggctcg tggaaatcga ccggatatga
     1381 tgtacgtatt gaaagtacag caccatatta cggccctcgt tccgatgtcg gattccggcc
     1441 tgtaatcacc tatacaggaa aaccaaaaaa ttaataatcc ttcctaataa tcatttaaat
     1501 tcttgagtca tgaaaacaaa caaagtcaca tgggttgtct ttttactgac ggggttcata
     1561 gtattttctt cgtttgcccc aggaaaaaag aaaactgtaa aaacacccga cggctatgta
     1621 tttgtgccca tgggcgcttg tataattgat tcgaacaaag tgaccattca ggcattcttt
     1681 atgtcgaaga ccgaagtcac caacaagcag taccgtactt ttctggccga tctgaaagcg
     1741 agcggcgaca tggaagctta caaaaaagcg ctgcccgaca ctacaaaatg gcaaatggac
     1801 ggcaacagct acaatcagcc gatggttgaa ctgtatttcc atcatcccgc ctacgacaat
     1861 tatccggtag tgaatgtatc gcgcgaaggc gccgagatgt attgtcagtg gctgaccaaa
     1921 aaatatgaag ctgagaacgg accgcagaaa tgcgccttcc gtctgccaca gcgcgaagaa
     1981 tggatctggg ctgcacgcgg cggtctcaaa atgattcctt atccctgggg cggaccctac
     2041 ctgcgcaatt cgaaaggttg ctatttatgc aatttcagac cattgaatga aaaacatata
     2101 acgtacaacc acgaaaaaca gcaatacgag ctggttccgc atgccgctga aagcttctcg
     2161 gaagatggtg cctttcagac aatcatcacc ggacattatg cgccgaacga ttatggtctg
     2221 tacgacatgt cgggaaatgt ggctgaaatg gttgcgcaga gcgatgttgt ggtcggtggc
     2281 tcgtggatgt cgcctggtgc cgatgtgcgt atcgaaagtg tggctcctta caccggaccc
     2341 gcaccaactg tcggtttcag accagtgatg acgtatctgg gagaatagtt ttaaaaaccc
     2401 aaaaaacaat cgcgcggatt tgccaaccgt gcgattgttt tatatctgtc acggatttca
     2461 aatcggcgcc agatattaag cagattatta actttgaaac aaaaaaaaca tgagtcgctt
     2521 tttatctttt cttttttcac tgatggctgc aacagcaatt tttgcgcaga cttcagccga
     2581 cttcactttc gaaaattcgc caaccggcaa tgcacttcct gaacatggtt ttctgtgggg
     2641 caacaattat tcctgcacca ttcagaatat aataaaacac gaaggcaata atgcgctgga
     2701 actcgtccgc aatgccaatg cctctaataa agattttgga tgttttgtat ttgccattcc
     2761 ggtcaatttc acgggaaaaa tggctcagtt caaggcgttc atgaaaacag aaaatgttga
     2821 caatggcatt gcatcgctct tcgtgcggat cgacggcagc agcaacaatt attatttcac
     2881 caacagtgtt tcgaatttat atggaaccaa tgactggtat cagttcaata caccgcagtt
     2941 ttcgcttgga aaaggggctg aaacgatgta tgtcggtgtt attttaaacg gcagtggatc
     3001 gctctgggcc gatgattttc aactactcat tgatggaaaa gattatctga aagcgccaaa
     3061 aataaaaggc gccgatttca aggcagatca ggataccgcg ttttcaaaat caagcggaat
     3121 tgatattgga atcagcaacc gtgtgcaggt cgataacctt gcattgctct gcaaagtatg
     3181 gggatttctg aaatattatc atccttccat tgcaaaagga aactacaact gggacaatga
     3241 actgtttcgt ttccttccaa catatcttcc cattacggat aaaaaacaac gcaatgattc
     3301 tctgctgtca tgggtgaata aactgggtcc tgtgaaaggc aagggaaaca aaatgaaaat
     3361 taaaaagaac acaaaactaa cgcccgaact cggttggatt gctgatacaa caacgctggg
     3421 atccaatctc agtgcggctc ttgtgaaaat cagaaattcg aaacgcgggg caactcatta
     3481 ttatatttca ctgcaggaca atgtcggcaa ccctgtcttt gaacatgaac gcagctacag
     3541 gacaatcagc tggaacgacg atggattcag gctattgaca gtgttcagat attacaacgt
     3601 cattgaatat ttctttccga accgtcatct gattggcgag gactggcaca atataccaaa
     3661 tgaattcatt ttgaaaatga ttgctgccaa agatgaaacc gaggctcaga tggtcattct
     3721 ggaaatgatc acacgaatca acgacacaca tgcatatccc agattcggtg aggaattcag
     3781 caatgccttc tatggaagaa actatgcaaa ctacaaactg acatttattg aaaacaaagc
     3841 ggttgtaagt ggatatctga acgagtcgat ggtgaaagat gatgatttgc agattggtga
     3901 tatcattatt gatattaatg gtgagactgt aatgcagaaa gtggatcgat taaaaaaata
     3961 tgttcccgct tcgaacgaac ctcaaaggct ttatcagatt tcgcgttatc tgctccgctc
     4021 cagtaacagg ggtataacag tcacctatgt acgtgatgga aagaccgctt cgaaaagtct
     4081 gccttgtcat acgccagaga caatattcgc caaaaccaat tccggagccg aaagtgctac
     4141 acccacctgg cgttttctga cacccgaaat cggatatata ttcatgggaa ctatcaacag
     4201 caaggagctc acaaaaatga tgggagaact tcgtctcacc aaaggaattg ttattgattt
     4261 gcggtgttat ccgagcgact ttgtggtatt ttcactcagc cagtatttta atattaagag
     4321 tactcccttt gcaaaatttt caaatggaag tattcagaat ccgggcatgt tcaccttcgg
     4381 aaaaccgctt tcagtgccag gaatgaaaac cgcttacaac ggcaaaatag ttatccttgt
     4441 aaatgaaaaa acaatcagtc aggctgaata caccgcaatg gctcttcgtt ccacacctat
     4501 tgccacagtt gttggaagca ccactgccgg tgccgatggc aacatcagtc cgataacgtt
     4561 gccgggtgga atcgtaacaa acatctcggg cattggcgtg tattatcccg atggaacgga
     4621 aacgcagcgc gtgggcattg tgcccgatgt ggttgtaacc cccaccctgc gcggttttat
     4681 agaaggccgc gacgaagtgc ttgaaaaagc cgtgcagatt attgaaggga aataaattca
     4741 gttgtgcgaa gtttataaac ttcgctcaac tttttaaaat ttattacctt gcataaaaat
     4801 ttatctatga agaaaattat attttctttt ctgctttgca tgctgtttat caccgcgacc
     4861 aaagcacagt cggtaaacgg aatcgtactg gccgacaccg ccaactacaa agtcattcag
     4921 gtcttcggag gccactacca gcgcggattc gcgtatggat acctttgtgc tgataacata
     4981 atggatatct gggaaaacta catccagccc agctatggta tgtatatgcc ctttgcggaa
     5041 gccattgtcg ggaacccggc caccttcagt gtagattcgg aatatgtcat tgaagcgcgc
     5101 ggaataattg atggcgttgc ctatgccggc tacgacacaa ccggactcag ttatctcgat
     5161 ctttttgtag tgaatttcat gaccgatctc gaaggtttca tgtcagtcaa aggatttccc
     5221 gaaacacaac aatgttccag tctgatgaac tggggcgccg caacacaggg aaccgacctc
     5281 aacggaaaag ccgtcatctc gcattttctc gatgctctga cactcaatca ggtgatatca
     5341 cgcaatcagg tcgtcgttgt gcattttcca acagaaaccg atgaacaacc atggctcatg
     5401 accggtactg caggacaaat ggtggcttcg caggctctca acaacaacgg acttgtttta
     5461 tttctgaata ccgtcaatgg ttttaccgcg cagcaaaaca aagggtatga acccatgacg
     5521 ctcacgctgc ggaaagcggt cgaaaaaaac gatttcaacg gcgacggcca gacaaatgtc
     5581 aacgacgtga aagccgccat tgatgccaat accaacgggt atgcaagagg atttatcgta
     5641 tctgccattg caccttcaac cattggcaac gactcactaa tcgcaatggt ggccgaatgc
     5701 gctccgactg ttccatacat cacctaccgc aacatcaacg atgtcgattc ggttgacgga
     5761 acaaatctct atgccgccaa caattttatc aaacgcaata acgcgcagga ttactgcagt
     5821 cgctacaaca acgtaaaaga ctctattaat cattactttc acggagtaaa tatcggatca
     5881 gttgacaata aagaaatcat gtacacccaa tcgacgcaga gctcatgtct gcagttcatt
     5941 caggtgattc ccgaaaacgg gatctttacc atgtcggttg caaataatgc cagcccggcc
     6001 tacacacaaa cgccggccac actcagttac agttaccttt tcaactatgc aggccttcag
     6061 gatctgacaa caaacaacac catatatatt tatccgaatc cggctacaga tctgatttac
     6121 atggatgtcg caccggaaga aaattattca tattacattt ccgatttatc gggaaaaatc
     6181 atttcgggcg gacaactcag gcatcagaac agcatctgca tcagtgcgct gccagcgggt
     6241 ttatatttta tacaacttgt ttcgtctaaa aaaatgctga ccggaaaatt tctgaaacaa
     6301 taatcggtgt ctttaacggt gctaagctta agctttgcat agcggggact atatctcaaa
     6361 ccgcttccgg atctcttcgc cgtaaacctg gccgctgatg agctttgtgc gcgccttgtc
     6421 attcatcaca aacacaaaac gtcccctgaa atatttctga atttccagaa tacaatcgct
     6481 gttgactatc tccgattttg aaattctgat aaacttttcc ggaagcttct gcgaaagcaa
     6541 agtgagcgtc tgatcagtaa aataggtcgt tccagccgtg ttgaagaagt tcacgtattt
     6601 gtcattcgct tcaaaataca cgacctcttc cagcttgaca ggtatgattt tatttccgag
     6661 tttatgtgtt attgttaccg gaacaggttt ctgaatcacc atgttttcta tagtgcgata
     6721 aagctctggt aaagctgatg aattaaggcg ttcaagcttt tgtaatgtca tgtcaagccg
     6781 atctttttcg accggtttca ataaataatc aaccgaaaaa gtattgaaag cttccatcgc
     6841 atacgaatca tacgccgtac aaaaaacaat ggctggcttg tggtcaagcc cggtgagcat
     6901 ttcgaacaca ttggggcctg gcatctgaat atcgagaaac accagttcgg gcatcagcat
     6961 ttcaattttc tttcctgcat caaaactgtc agcagcctca tcaatgattt caaagcgacc
     7021 atcatatttc cccagcagcc ttcgaaggcg ctcgcgggcc ggcttttcat catccacaat
     7081 aagtgttttc catttattga acatgtcttc aactatttat tcaacgtaat tacaactttc
     7141 tttttcggcg aatttacaaa ctcaatgtca aatggctctt tatacagcag ctttaatttt
     7201 tcgtacacat tctgcagtcc ataaccattc atcagtccgc ccggaaattc aggcccgtta
     7261 tcagccactt caaaaacaat cttttcattt ttctcaaata tccggatttc gataatgcct
     7321 tgtcctgcca gtttcgaaac accatgtttc acagcgtttt cagctaatgg ctgcaacaaa
     7381 aatttcggaa ccttaacttg tcggagagac tcctgtaaat caatgctaaa aatcatttta
     7441 tcttcaaagc ggattttttc aatctccaga taggtagctg tcatctctgc ttcatcgttg
     7501 atggtggcat acatgtcatc ttccttattc agattataac ggaagagttt cgatagcgac
     7561 agcgccattt tttccgtttt atctgcatta ctgtgagcca gcgaagcgat tgaattgagc
     7621 gaattataca gaaaatgcgg attgatgcgt gcctgtaatg cattcagatc ggatttgaat
     7681 ttcagctctt tttgcttact cagttccagt tccttttgct gcagcaatcc ggcaattttt
     7741 tgcgactgca ggcgaatata cattcgatac agaacaataa gaaatccgac cagcaacacc
     7801 tgccaactta tttccgacaa agaaatcagc tccggtgaaa taaaaaaata aaccagctga
     7861 tatgaaataa aaagcacagt aaaagataat attaaattga aaacagcagc cagccatgtc
     7921 ttgttaattt tcttcaaaag cggttctgca gcaacaaaca agagagtgca aaacacagca
     7981 aacggaaaaa taataaactc cacaaaaagc aacaaattcg cttcgtgata atcgcccgcg
     8041 agaaattttt cggacaacaa aatcagaact gccaataccg gaatctgagc cagcaaaagc
     8101 gcttccagaa gttttcgtgc aatgccgaaa cgccattttt ttaaacgaat atcaagacca
     8161 taatatttat acagctctga aaataaaaag aaaagacata ttgacaatat cagcgtaatt
     8221 ataaatacct ccgttttgct ttcaccttct tcatttttat taagcatttg tttcacctta
     8281 tattcaccgc cttcggaata caaaagactg agcatgtatt tgtcaagccc ggacatgttt
     8341 ttacctccat cgaagattga actatccttc ccaaaagaat gataagacac tttgaaattt
     8401 cctagctgtt caacaatact acgtttcaaa tctgcaacag aataacccca tgtatttggg
     8461 ttaacgtaca tgaccatcct cttgtgaaaa gggaaaaata tatttctgtt gcgggcaatg
     8521 tatcccgcac tggttgttgt aaaaccctta ttaattagta ctgaatccga aaatgttgta
     8581 tcaaatatta ttctcagatt tggctgataa tctgtcactt ttacttgaat tgttttcaag
     8641 atttcgttca gttcagaagc aatgtcattt acagttttga tatctctttc tgctgccaga
     8701 ccttcgattc cgattcgcaa atctgtattc atcagattca aatctgtact gtttgatgaa
     8761 aatgaaacta cttgttttac aaattcattt tcataactgt taatgattct aaaagatacc
     8821 tctttctgca ggatttttat tgtgccatcc ggcgctctta gcattatctt ttcgatccat
     8881 atttttttgt tattgtcaaa tccaacatcc tcttttatcc gggttatagg aaactgagct
     8941 gtctgcatgg ataaatcaac cagatctcca ttctttctaa tataggtaac aacagccgag
     9001 acaaactgaa atacaccaaa agtgctgtca ataatcaaac tatctgaatt gaggaagtcc
     9061 tccatggaaa tgacattttt ccaccttcgc ttatctccgc tcaggtacac atcgggctcg
     9121 ggaacaggcg acacatcgag taaaaaaaac gtaccattaa ctttaagttt caagccattt
     9181 aatttcattg gattcaccag atacctattt ccgcctaact tttcaatgac gccacctgtc
     9241 acagtaatat tcaccggaac agagggattg gatgtttcaa tttttataac cgatggcaca
     9301 taataatgta gaaaatcatt ggcaatgctc accctgattc cgtttccgct gttggtcaaa
     9361 atccatgtcg tatcgcctgc ggctgcttct gaaaaaaaag aacaagccag aaaagacaga
     9421 aataaaagaa ttcttgtttt cataatgaat gattttgctt tgttgatgca aagtaaatcc
     9481 gccgtgaatg tacataaaaa ctatttctga caaacatgca tttttgcgga atgaaattac
     9541 atctccgctg tgcgaagtta aaacttcgca cagcgtaatc ttcgcacaaa cctctatctt
     9601 tgcacaaata tcttccccca tgccccgcct tctcctcctt ttatcacttc gactggctca
     9661 gtgcaccgct ttttcctttt ttcttttatc ttctgctcag gcgcagaccg acagtgtctg
     9721 gtacaacagc gtcatcgcgt ggcaacaaga aatgaacagg gaatatgccg attcggccac
     9781 atcgcctctg acacccgaag atcgtgcaat atttaaagga ttgccgtttt acgcacccaa
     9841 tgcagatttc tgtgtgaatg ctgttcttga aataacacca aacgcgaagc cattcatcat
     9901 gaaaaccagc agcgcaaaga cgccaaccta tcggcaatac ggcgtttttc attttaaaat
     9961 caaaggacaa tcattttcac tgcccgccta tcaacgtgta gaaatgctta cctccgacga
    10021 atatgccgat tatctttttc tccccttcaa cgatctgacc aacggcgaaa gcacctatat
    10081 cagcggacgc tatatggatt gccggattca gaaagacaac ctgtacattc tcgacttcaa
    10141 caaggcctat aacccttact gcgcctataa tcacaaatat tcatgtccga tcccgccagg
    10201 ggaaaatttc ctgaacatcc ggattgaagc gggcgttttg aaataatcag tgatgtgcga
    10261 cgttgaaaaa cgtcacacag cgccgttagc ttcgcacaac tttcaaatat tgctcaacag
    10321 caatcgggaa ccatttatat tcgagctcgt ggacgcgtgc tgccagcata tccggcgtgt
    10381 cattttcaaa gaccggacag aattgctgca tcagaatttt tccatgatca tatatttcat
    10441 ccaccaggtg aatggtgatt cccgattgag tttttcctgc ggcaatcaca gcttcatgca
    10501 cataatggcc gaacattccc tttccgccga aatcgggcag cagcgccgga tgaatgttga
    10561 taatagcttc aggaaagcgt ttgataattt cagccggaac catctgcaaa aaacctgaca
    10621 atacaatata atctgttttc cgcttcagaa gcttgtttaa aacaacatcg ccttcgcgga
    10681 aatcttttct tgaatagaca aaacaatcca ctccaagacg ccgtgcacgt tcaatgacac
    10741 ccgcttcagc attgttgcaa agaatatgat cgatacggat ttcggaatgt gatgaaaaat
    10801 actgcatcag attttcagca ttggagccgt ttcccgaagc ccagacggaa atgtttttca
    10861 tgtgtgaaaa agattaattg ccgattttat taacatgtca aaaaaaacgg aacaaaacag
    10921 ctttgttatt ttcattgggg tatattattt tgaagcaaaa gcaatttccg gtattttttg
    10981 aatgaagctc cggccgataa ccacgaaatg gtaaatcctg ccttaccatc aagaatgcca
    11041 agtttcagaa aataatcgcg gataaattta aaaacagttg cggcaataat tccaggccac
    11101 gaagttctgc gacccgtatc aaacatggat ttcgcaagta tatccgtaaa tttctgaatc
    11161 tgcttcaaat gatcttcgcg ggtgtagtag ctgtaatgca gcaggtcgcc ctgcaaatgc
    11221 ttgatgacag aacctggctc catttcgtag cgatcatgcg gattcagacc ggtccattgt
    11281 cctttgcggc tatcccacag tcgcagtttc cgatcaggat accaaccgca atgccgcacc
    11341 caatgcccac aatagttggt cagacggttc atggtgtatc catccgcctc aaaattttct
    11401 ttcactttac gaatgttatc acacagaatt tcgtttggtg cttcatcggc atcgagtgaa
    11461 agaacatgtg gatatgcggc ctgtgtgatg gcaaagtttt tttgctgaat atgtccttca
    11521 aaagcatgtt caataaagcg cacgcctttt tcgagacaga tttctttggt tcggtcttcc
    11581 gaaaaagaat caatcactac aatatcgtca gcaatatcac gcaccgaatc aatgcagcga
    11641 ccaatgttgc gttcctcgtt gaatgtgatt atgacaaccg acaggctgat tttcatgttg
    11701 caaagataag aattagcttt cagctgagga caagaacaag gtttagtaag actttcgtta
    11761 cacgtggaat ttatcacgct gaaagcggga atcgttacgg gatggtgtgg cctacggcat
    11821 aaaagaaagc tacgacgtgt cacagattgt caggttttac gtgaggtctc aggtaaaacg
    11881 cacacagcaa caaaaaaaaa ggaagtcaag atcaaggaca aaaataagaa atgaaccgtc
    11941 tttcaacttc ccagcgcaat ggtaagaccg atattgagta acattttacc gtatcctatt
    12001 tcggcataaa aagccgtatt atcggaaaaa taattacggt agccgaaaat aagttcaccc
    12061 gaaaaaggaa ggggccggtt aggcacacgg gatgtggcaa gcggatcatc actctgaaaa
    12121 ttcatcatat aaaactcgta accggcaccc agtccgatgt aggtatctct gtttctggta
    12181 gtggcaaaat gaaaattcat acgccccagt aaagcaagtg aaagaccgga atatttttcg
    12241 gcatatacaa ccggttcgta agtccaggtc tcattattgt aaacatcaac tgttttattc
    12301 cacataaaag ccggcaggtg aaaaaacact gttcctccca gaccaatata atcactgatg
    12361 gctttttcat aacgaaaaaa caaaggcggc actccaacac ttttaaaatc ctgcaacacc
    12421 gcatgtttac gtggtactgc attgacaaga ccatataatc cactcccaaa gctgataaac
    12481 gaatcacctt ccttaaaaca aaatctcctc atcggactac tgcatgaatc gggctgtacc
    12541 gcaaacaaag aaaaagcaaa tgcggacaga aaaataaata ccagcggctt taccatttgt
    12601 tgttttttac agctactaat atacacaata tatttccata aaagcaaatt cgattatttt
    12661 tgtatcctct gatgaaacaa caacttttat ttcttttatt tctgacagtg gtttctgttt
    12721 cgctaaacgc gcagcagtac tctatttcag gcaaagttac cgatgccgaa accggaaatg
    12781 ccctgccctt tgtcaatctg gtcattaatt cctctccaca tggctctatc agtgatatcg
    12841 acggaaattt tattctgaaa tccaatgaac caatccgcta tatcagagca agttatgttg
    12901 gctacaaacc agacaccatt gaaaccaacg gaagtccatc tgtaaatttt tcacttcaga
    12961 aatcatccat acaactcaac gaagtcatta tcctgccagg ggaaaaccct gcacaccgca
    13021 tcatagacag tgccattgcg caccgaaaag aaaacgatcc ttttcaactt gaagcatttg
    13081 cataccaatc ctacagcaaa tttctggtaa cagccgaaat ggattcacta atgtcaattc
    13141 ctgatgaact gctagactca gctcatcttg aactaaaaca ttttttttcc aaacagcatg
    13201 tttttctgat ggaaaatatg gccgaacgca aatttctcaa gccgggcaat gataatgaaa
    13261 tactcactgc atcaagggtt tcaggttttg gcgatccttt gctgacactg cttatgagcc
    13321 aaattcagtc tttttctttt tacaaaacgc tcattaccat ttccgataaa aactacgtaa
    13381 atccaatcag ctccggcagc actgataaat atttcttcct gctcgaagac accatctatg
    13441 atcattcaga tactgttttt gttatttcat accggccgcg gcgcaatgcg catttcgacg
    13501 cactcaaagg gcttctttat attcatacgg caggatgggc tttgcagtgc gttattgccg
    13561 aacccaacac cgaagaaccc ggtatgtcgg tgcgcataaa acaaaattat tcaagacccg
    13621 actccattca ctggttccca tcgcagctgc ataccgatat attgttcagt tctctgaaag
    13681 tatatgaata tcctgtatat ggacatggcg taaccgaact gaaaaacatt caaatcaatc
    13741 cggcgctgaa aaaatccgat tttgctgcag caaaagtcat tattgaaccc gatgcaaaac
    13801 tcaaagacag tcttttctgg tcactgaaca gaccgcaacc gctttctcaa aaagaaataa
    13861 acacctatcg tctgattgac agcattggtc gcaaagccca tttcgaccga ttgctcgata
    13921 ttacaaccac actactgagt ggctttattc ctgttggacc actggccatt gacatcaatg
    13981 ccctgtatcg gtataatgtt gcagaaggaa gtcgtccggg attggggtta aaaacaaatt
    14041 accgagtttc aaaaacatgg tgtctgtatg gatattatgg atatggcctc cgcgacaaag
    14101 aacacaaatt tgcaagccgt atcgacatta atctgaaaaa agcaaaagac acaaaaatat
    14161 cattcggatt taaacaagac atccgcgaaa ccggttcgat gaataatgat ttaaacacca
    14221 atggaatttt atcactggaa ggactgcgta attatatgat cagccggatt gattatttcg
    14281 atcaggcaca tttcgacttt cattcacgca tcggcaggtc atggacaata acatcaaggg
    14341 tttctcgctc cgcattttca gcacctgatt ctgtattata cggcaactat aatgaatacg
    14401 gatttgcggg cacacgctct tttatattaa gcgacataaa tgtttttctg gtgtattcaa
    14461 aaggaatgga atttattcaa tccgaagact attcgttgcc cattggaggc aacacatcaa
    14521 atccggtcat cacaatcgat ctgcgccaaa gtttaaaaaa tattgccggc ggtgattttc
    14581 aaagaacttc ggccatgatg cgaatttcgc aaacactcaa atcgcgctat atcggaaaat
    14641 tttcatggat gcttcagggc gctgtggtaa gtcccgacgt gccgctatcg ctggctttca
    14701 atgctccagc ttcatacaga cacttcactg tttcatcgcc gaactctttc gaaacgatgc
    14761 gtatgaatga gtttttcagc acacagtgtg tatttctttt cctgcgtcat tccttcgaat
    14821 cactgctgtt cggcaacagg aaattctcac caagcccaga gctggttacc aacgttgcct
    14881 ggggtgatta caaaaacact acacatctca atgttccgga tttcaaaaca ctatcacgcg
    14941 gctactttga atcaggactg atgatcaacc gcattctcga cgccggattc tactcactgg
    15001 gggttggctg tctgtacaga tacgggccat atcgcctgcc tacgctgaaa gaaaatctca
    15061 catttaaact gacattgaac tatcatctct gacaagtttg ggaatgcgga tggtaaaaac
    15121 ggttcccttt tcgcggttgc tgataaaact gattttacca ctcagataat ttgtggccag
    15181 tttttttgca caataagtac ccaaccccat tttgccatct cctcctgccg caaatcgtgt
    15241 aaaaaccagc ttttgttttc tttcatccat gaccgattga tttttgattt taaacgaaat
    15301 cgttgcagta gaatcaataa cggaaacaag aatttcgcta cggtcatttt cggcctccat
    15361 tgcatttttc acgagatttg aaagaacata tccaagcagc atgcggtttg aaaaaatcat
    15421 catatccgga atagcttttt taatatattt caatcgcaaa tcctgtctaa gccgtaaatt
    15481 ggtaataact tcatcaacaa gttgctttac cgaaaaccat tcctgatgac ctggcaacgg
    15541 ctcacctttt tcaattttct gaagaatttc aaaattattg attgcttcat ctatttgcat
    15601 cagctgagct ttcatcaatg gctgaaattc ggcagcagaa aaatgaccgg aagcttttac
    15661 caatgattgc attgaagaaa gaatggattt gagattggtg gaaaaaatat tttcaagcaa
    15721 atcacggtaa tgagtatcaa ctctgttttc aagaacacaa aacatgtatc tgtttgccat
    15781 gtgttcaaaa ggggttatgg ttacccccag attataagtc tctcctgatt tattcaatag
    15841 atgacaatca taggtcgatg ccggtttacc aatttcagca tcgaacaagg cctgattaaa
    15901 gccacaatac ttacaaaaaa ctgaagcacc acagccgctg cggcttacat cagcatattc
    15961 gcaatgcatg gcctcgccag ggcgctttcc aagtagatcg gaacaatcct caactccaag
    16021 aaatttttcc atgcttttgt taacgtaaac aatctgacgg tttcgcgtta aaaccatcac
    16081 catcggagaa atgtttttca acaactccgg catgtatctg ttcatcagta caacatgctg
    16141 ttgtctgaca tcagataccg actcgcgtgt aattacatca aacatagcta ttaattttaa
    16201 tggtaaatta tttaggaatt taactttcca attgaaccat taaaattata gcatgaaaac
    16261 taaaaagtca atagatgaac aaaataatac aatgtattta acatttttat cgacgaatta
    16321 gtcttctttt tcgaaatagg ttttatagaa attcagtttc ttttcttcgt caaacccgcg
    16381 ctcaatccat tcccggcgcc catgaacata gatatgaaag tttgtatcca gttttataac
    16441 acttttaaag tgcctggcca tctttttaac agctctttca ttcaccagaa aattttcacc
    16501 catgttcaga tcctgttctc gggagaattg tttcgcaaaa tgggtgaagc tgtctttaag
    16561 ttccggttgt ttcaatacat cttcctgaaa atttttcaaa gcaaattcat cgctggcttt
    16621 aaagaaatcc atgcttttta taagcaattc ggcctgatcg ggacgagtgg tttcgaattc
    16681 ctccggcatt ttttccatca caaaatcctt acacatacgc atagccagct gtgtacgata
    16741 aaaactatcg atattttgtt tcaatatcat aaaaacatcc atccacatgc gcaaatcaag
    16801 actcttgctt ttgccggtag ccgaaacaat aaagccctcg tcaagccccg tattgcaaat
    16861 caaacagccc gataaaaatc ccgaaggatc agtccctgat tgcatttcga tggcataatt
    16921 ggtttctttg ttatatagtt tcacaaaatt cttgcgctca tccatgagat aaaaggccac
    16981 ggcatcagtt tcttcatcat caacaacaat tcctttaaaa taagccaccc agaattcgcc
    17041 gccatgcaac ttgggattct gcatcaactc atacaattcc ttcgctgcag ccacactggc
    17101 ttcgataaat tcctcatcgg gcgagcggaa aattttcttg cagcgggtaa acatcacatt
    17161 cagattgaca tcagtatcgt gataaaaatt atataacaga tcttcattac caaacggcac
    17221 cagcaaaggt cctcctaacg agctcagata agcttcgctg agtgaaacaa gttcatcaga
    17281 cagcgtgagg ccgtcctcca acactttgtt tcctactcga tgtataataa gcttcgacaa
    17341 ataagcgttt tcgatattga tcatggggtt gttttttgca aaaatacaaa aacaacgctt
    17401 ccggaacaag caactctatg accaacatat ataaatagat cctgctgaaa aaaatgttat
    17461 atttccctga aaacaacaac acttctgaaa gccgcatttc cacttgattg atgctgtgcc
    17521 tcgaaattaa atgttttaag ttccggccat tgatattttg tctgaattct ttctcccggc
    17581 aatgtttccc ggatataatt cacaataaat ttttcgaaaa catgctgccg ctgaaaatca
    17641 gccggaagtg cattcataac ccattccaga tatcgggtcg aggttacgtg tccattcaga
    17701 tcaaaatccg taaaacaagt ttcaacattg tccgtgcttt tcgaacagaa agattgaatt
    17761 ttttcaggag ccgcttcaat ggcctgctta tcgcggagag aaataaacat ccattcgtct
    17821 tcaagatcaa ttttaccggg ctttttagtc tgacggtcaa tggccagcca gtgcgatgtt
    17881 gcacgacccg taattacgcc attttgcctg atcagaaaat cacggcgata cagaattttt
    17941 tcaatatcac gtggccatgt ttctatttca atttcatcgt accattgagg tatagttttc
    18001 atttcaagtg taagacggct caacacccaa aataattttt gttttttcag atcacgaaag
    18061 ccaaaaccca tccgatcggc cgaatcgatg gccgactgaa tcagaaatcc aagcaaagct
    18121 gatggcttca gtcgcgcata aatatcacaa tcggccgatg tgatgcgata tcctgaaacc
    18181 aatgctaatc cctctttact aatatttgtg atcatacaaa cgaaaaaaca ttaccgtggc
    18241 tcatcatcat tttcacaata cgaatccggc tcctgttttg gcttcgatgt aagaactttt
    18301 ataaaaaagt aagcagtaat agcagtaact gctgatacag taataagcaa cattatcaat
    18361 gctcctgtgt tcatttcaat ctcccttctt ttttatgacg ataataagct tttcgaacca
    18421 ataaagcgat tccgacaaat acagtcagaa gcaacaatct ggaaagattt gttaacaaca
    18481 aacgatattc aagatcggca atgacagcgg gatcagtagc taccgcaatt ttttccttca
    18541 aaccattgtt cattaatttt ccgacaagac tctcatgatc aaacgcccat ccctgaccgt
    18601 cagacaatcc ctgaaatgca gcaatccagt cgttaccgac aggagtaaac acagcaccaa
    18661 gaaaaacaat aatcaataaa accggagtta cataagcaat taaaaaccta aaaacacgac
    18721 tcactttaat atcggcgcct gtatttattt ctttccatcc tttctttatc ccaaagaacc
    18781 aggcaaacaa aacagcttcg aatgcggcaa atacaaaaag cgcaaaagaa cccgtccagt
    18841 aatcgtattc atcaaaaaca ccctgattga aaaacaaaac ggtaggcatt ccaataacca
    18901 atataataag tccgaaagca agagccgttt ttttgcgcga ccatttgaat tcgtcttcga
    18961 gaaaaccctg cacaggtgtc cccatagcca gtgaagaagt aataccggca aaaaacaaca
    19021 aaccaaacca taaaacgcct gcgatagtag ccattaacgg cccccattgc gaaaacaaat
    19081 aaggaatggt tttaaaacca agaccaagac caccaagtcc cgccagttcc tgcactttgg
    19141 caacacccag atatcccaca gcaattggaa taatgacggc tgcacctaac actacttcaa
    19201 caaattcatt catccagcca gccgacatcg cattaagtgc tatatcatct ttttttcgca
    19261 aatacgacgc ataggcctgt atggatccca tacccaacga taacgtaaag aatatctggc
    19321 tggctgctgc aaaccaaact ttcggattat aaatagaatc aaaatccggg gtccataaaa
    19381 aattaagtcc aaccgagctg tcgttgatgg caccggcagt ccccgccttc agtgtcactg
    19441 cccgaatagc aagcagtaaa ccaaatacaa gcaacaaagg aactccaatt ttggcgactt
    19501 tttcaacacc tccactaagc cctttactca atataaatac attgatacca agacaaatca
    19561 aaaagaaaac aatgggctca tacggaatca gggttgcttt atctatcgac acataatcat
    19621 tgaacaattg caccacctga tgctgatcca taccattgaa agtaccaatt acactatgaa
    19681 aaatataggc aagtgtccag gattccatgt agcaataata agatgcaatg ccaatattgc
    19741 tgaaaatgcc gaagaccccg acatatttcc aaagccggcg cctgtccata ctctgcatca
    19801 taaatggagt gctgtgaaaa ccatgtgaac caccgtaccg gccaatactc cactcgataa
    19861 aaagcaatgg caaaccgagc atgagaaaac taaccagata gggaataata aaggcaccgc
    19921 caccgttttg aatggcctga accgggaagc gcagaaaatt accaaaacca acagcattgc
    19981 cagccatggc aagaattaat cccacacgcg ttccccaggc ttgtttggta tgagtcatat
    20041 cttacttttt actaaaacaa ttaacaaaaa cacaactggc tgttttgcaa acagtcttgg
    20101 aaatattccg taaaatatcc atgcattcat tcatggttat ttgagcgtat aagttcattc
    20161 atcaccggac aagatgcgca tttacctgga gagcagaacg tatcaaacaa ctcaatcaaa
    20221 ccctgcgact ccaaagcatt gcagactttt atcccggcat tccgcacaaa tatatcaata
    20281 attttatttt gctccggtgc aatacttttc aacagaaacc aggccttgtt ttcataactg
    20341 cggttgccgg tgtactccga aaatgcgatc aatagcggta caacggcatt aatcaaaatc
    20401 aaatcgacgc tggttttacc tatttcacca tcaccaccat tttttcgttc tgtttccgga
    20461 cgacaatgac ttttccaata accagaaaca ggttggcgca aaatctggta aaaatctttt
    20521 tttgtatttg cctgcgttaa ttcactgaat aattccggaa ccagactcac aagtgctgcc
    20581 acctgtgcca atcgtgactc cggcatagcc gaaggccgca ccggattgta tttccaggca
    20641 ttcaaatcaa cagggctgag cataaacata tttttcctat gaagatattc ctgcttaaga
    20701 gctaacaaat aaccattttc agaagatatt tccttatcga gaaatccccc ttgtccaagt
    20761 aaaatggctt caatggaaaa cacatttcca gccctgcgca ggtttttcca tgatattgta
    20821 ttactgagcc attccatggg aaccatattc tgattcagac caaaagacca cgcgatagct
    20881 ttccatgttg tttcgggcca atcaaaatga ttgttttcca gcaacgtttg tatcctgacc
    20941 gatttacgga taattctttc cttagccagt ttttcagcca atgcaagaaa ccgctcagga
    21001 tctgccgcag aaatataatc actgcaatgc aaacggccat aaccaaacat gcttttttca
    21061 gaataattct catataagcg cgtgatttca tcggccggaa gcacaataga aggaatcaga
    21121 caaccatcct gattgcaaat aatagcatcg tttttcagca caatatgcaa gatggtttta
    21181 tcataagccg gatcggtatg gtgcccatga cgaaaccaat cgctgctgca aagatgaatt
    21241 tctgcatttc cggccaaatg ctgctgatta atgacaattt cagtgttttc aaaatccggt
    21301 ccgtcacgat tacctctttt tccgggtttt aaaatacggc aatcgtcgcc agtgtcgaga
    21361 ataaactgct gtccggcaag tcctttttcc cagacataat gaatgaaaga ttcaggtatg
    21421 ttcataattg gcacagtttt acgtttatat atatagcgtt taaaccgagt gattagtttt
    21481 tcggcacata ttgcgcccat aatataaaca tttcttttta aacgccattg tcagattttt
    21541 aataactttg caaaaacacc agccatgcgt acattattat tgctaattcc gtttctggct
    21601 ctcattatgc cgctttccat ttcggctcag agcgaagaac ccatttatgt agtagttgac
    21661 aaaatgcctg aatttcccgg tggccccaat ggtttgggaa atttcatacg atccaaccaa
    21721 tcatacccgc gcgaagcact ggatgctaaa attcaaggcg ttgtcattgc ttcttttatt
    21781 gttgaaaaag atgggaaagt gagcaaacca accattgttt ccggcatcgg acatggctgc
    21841 gatgaagaag taatacgact cattaaaatg atgcctgtct ggaaacctgg aatgaaggac
    21901 ggaaaagaag tccgggtaaa actgaatatt ccggttgaat tcaaacttgg ataataatat
    21961 tttgacatgc taaaaagatc ttcagactga agatcttttt tttaagtttt tacattgttt
    22021 tttttaaaca ttgatatctt ttaaatatat ttgccaaaaa ataaagactt atgaaaaaat
    22081 tattactgat gttgatggga attgtgttaa gctcttttat ggcatcagca caactcaaca
    22141 ctgaaaaacg cattgaaata gaccttcgca atgggtattc gggcgaaacc attttttaat
    22201 ccgtgcatgg atattttatt cttgaatcca aggccgatga ttatgttgat ggacaagctg
    22261 aaataaaata cgatctctat tcaggtgatt tggaactaac agaaaccaaa tcggttttgg
    22321 tacccagaaa aatgttgttc cgggaattgt attacaacga tgattacatc tacaacatgt
    22381 acgctaccaa agggggtgaa ttcatgatta ccagtgttga aatgaaaaca atgaacatag
    22441 aaacagccac tggatccctt cctccaaaag taattttacg cgacatgaaa gtcttgggaa
    22501 atcaggccta ctttctcacc tcaataaaaa aagccccagc tatttacaga gttgatttga
    22561 catcaggtaa aggcacaaca attccgataa ccattggagc tttcagcccg aaaaaaatca
    22621 gcagtaaaaa ttatcagatt cttgaaaaca ccaaagaaat tcttctgttt gcacgtgcca
    22681 caataagcaa ggcaacatca gaacttcata tgatcaggat ttcggaagac gacaatgtgg
    22741 acaaacccat taagatttca ggcgtaggcg acaatgccat tatctccatt tcgggatgcc
    22801 gtgtcgatgc cagcaaaatt atcattaccg gaacttacgc aaaaaaaggc ggattatccg
    22861 aaggcctctt ctttggagaa gttgaagaag gaactgttaa ttatatccgt tactacaatt
    22921 ttctcgatct gaaagatttt ctctcatatc ttcccgaaaa aaagcaggat aaaatagaaa
    22981 agaaaaaagc caaaaaagaa gcacagggca aagaaatgac gctcgattac tggattgccg
    23041 atcatgatgt tattgttctt gaagatggat acttattcct tggcgaggcc tattatccga
    23101 cgtatcgcac cgaaacttat accacttatg taaatggccg gcctgtaaca caatatagaa
    23161 ctgtttttga tggttatcaa tatacacatg ccacccttgc caaatacacc aaagatggtg
    23221 aaataatgtg ggatgtctgc tttgagatga atcccggata taaaccattt tatgtaaaac
    23281 gcttcattgc catttcagaa caaactgcca cacaaatcag catggtatat acgtcaggaa
    23341 acgaaatcgt ttcaaaaacc atggacttcg atggcaatat tatcagtgat gaaaaatggg
    23401 atttcattga aacaggcaat gccaatgaaa aaaccaaacg tactttttca aatgttgatc
    23461 attggtacgg taacttcttc ctcgcttatg gaagtcaggt tgtgaaaaac ggcaaagaaa
    23521 aacgcaaagt ctattttgta aataagattg gtttctgatg ctggaagaga cactgaggct
    23581 gtctcaaata gtcttcagtg acaaaaacaa cattccatcg tccggaagcg acgtaggagc
    23641 tgaaggacat gaaaccgttg tttttgcagt tgttacgacg gttccatgtt ccggcatcaa
    23701 tgtccttttt taaactcagc tgtatgccgg aacagtggaa tgtcgtaaca accctctatt
    23761 ttgagacagc ttcatttgtt ttcaatattg ctttccgtgg taagcattta atttgtatgt
    23821 ttgcacacca agataagcat ttatgaaaga aatatacgaa atttcggcac ggcgaataaa
    23881 cgctgtatca aaagaattca gcagaggtat aattaactcc gtcaaatgga ataatcgtct
    23941 cattggtatc cggggagcac ggggtattgg aaaaacaact ttattattac agcatattca
    24001 tcaaagtaat tataaaccgg aagaagtctt gtatgtgagt ttggacaatc tgtggttttc
    24061 gaatcattcg ctgacggatt tggccgacga ctttgtaaaa aaagggggga tgtttttata
    24121 cattgacgaa gtgcataaat atccagaatg gtctgccacg ataaaaaatt tatacgacga
    24181 ttacccggat ctgaaaatag tattcaccgg ttcttcgtta cttgaaatac tgaatgccag
    24241 agccgatctt agtcgccgtg ctgttatcta ttcaatgcag ggtttatcat taagggaata
    24301 tctcaatatg acattgaata agcattttga agcatacagt cttgaagaaa ttgttaaaaa
    24361 ccatgtttca attgccagta aaataaacaa ggaaataaga cctcttaaat actttaacac
    24421 ttatctgaag catggatatt tcccattttt caacgaagat cttgatgttt attatcgccg
    24481 tattggcgaa gtaattaata tgattcttga aattgaactt cctatgttaa gacaactgga
    24541 agttcaatat gttccgaaaa tcaaacaatt attgcagaca attgcagaaa cagctccgtt
    24601 tataccaaac gtaagcaagc tgagtgaaaa aataaacatc aaccgtaaca cactggcgtc
    24661 ttatctgatt tttctggacg aagccaacct gaccaacaac ctgtatcgcg actctaaagg
    24721 tatcagccgg ttacaaaagc caaataaaat atttctggaa aacaccaatt ttatgtttgc
    24781 gcttgcccct gaagaaataa acatcggaaa catgcgtgaa acattctttt ttaatcagct
    24841 ttcacatata catcggattg aattccctga ggacggggat tttctgattg atagaaaatt
    24901 ccttttcgaa attggtggaa aaacaaaatc aatccggcaa atcaaaggca ttgcgaatag
    24961 ttacattgct gcagatcaga ttgaatatgg tcacaacaac attattccct tgtggctgtt
    25021 tggttttctt tattagttac tgaaaaggat acatggtttt aaacaaatgg cgcggatgca
    25081 tatccgcgcc atttattttt cctatacaaa tttatatttc aatcgtcttc catttgccac
    25141 ggcggaacca ccacaacgcc agtaaagcca tgaatgattc agccaccaga atggaaatat
    25201 atacgccgtt cgatacggcc gaagtgttga acgaaagcca gatggcaagc ggcacttcaa
    25261 caatccagaa agcagcaaac atgaaccacg cgggtcgcag tgtatcgccc gcagcattga
    25321 tgctattgag catcaccgag cccagtccgt acacagccat gccgtagctg accatgcgca
    25381 aaccgcgtgc tccggcttcg atgacagcgg gttcattgat gaacaatcct attataaaag
    25441 caggaaataa cgccatcaat aatccggctg tacccagata ggccatattc aggtatgatg
    25501 ccgaccacgc cgattgttcg gcccgatgcg gttttccggc acccaggttt tgtccgacca
    25561 tcgttgcagc cgcattgctg aacccttcgg caggcaacag gaaaaacaac acgatgcgta
    25621 tggcaatggt gtagccagcc accaccacac tgccgaattc ggccatgatg cgcataagca
    25681 caatccagct ggcagtacca atcagatgct gccctgtgac tcccgcagcg agacggaata
    25741 tctctccaat ggtcttccag tgaaacataa tggcgcgccg tgttatccgg atcaggcgtg
    25801 ttccattgaa aagaatatac aattgcatga atacacccac cgaacgtcca atcaaagtgg
    25861 ccagggcagc gccttcgacg cccatggcgg ggattggtcc aaggccgaaa atcagaatgg
    25921 gatcaagcac tatgttgagt ccgttggcca ccaacagcac tttcatagct gttgccgggt
    25981 ttcccgacgc acgaaaaaca gcattggctg caaacaacaa cataatcaac ggtgcagtac
    26041 ccaacgtaat ggcggtgtag agactcatat tggacacaat gttttcttcg gcgcccatca
    26101 tgcgcaacag gtctccggaa aaaattatgc cagctgtggc aatcggaaca gaaaccatca
    26161 gcgatgtaat caacacctga aaagatgtga ttgctgcagc catgtaacgt tttgcccccg
    26221 aacgtctcga cacgatggct gttgcagcca cagccaaacc aataccaatg gcataaacaa
    26281 tggtattgat ggattcagtc agtcctactg ccgaaacagc atcggagccc agttttgaaa
    26341 caaagaaaat gtcggctatg gcaaataaag actccatgag catttcgagc accatgggaa
    26401 ccgacagcag aaatatggct ttggcggtgc ggccgctggt atagtcatat tcctttccgg
    26461 caatggcatc gcctatgagc gagcccaggc gccggatgcc ctgaaaatta acgctgaaaa
    26521 gagaaaatct gttttgtttt ttatatggtt tcatattgtc ttcgttttat gtgattttgc
    26581 atgcccggat cctgtcctgg ttttcttaca tacattgaac gaacaatggc cgctgatgca
    26641 gctttggtga tggtaaggcc gacggcctca aaatacagtg ttttcatttt tttggttttt
    26701 ggtttataca cggatttttc tccccgcggc ttcatccgcc ggagaatttc tttgttcaga
    26761 aagtctgttg aaatacggaa tcagcgcttt gccacaaagg tgacagcaag gatgaaaaag
    26821 ttgacagcaa gcgatttcat aaaattccgc aatggtttga ggatgcaaag ataaatatta
    26881 ttttaaaaag tatggaattt caattattca aatattgatc atccttaata atgccagtga
    26941 tcgactcagg tagtccaaca ttccatagtt cgcagtactg ccctgcagta taaaatccac
    27001 gaagaacatg gaatagttgg gcagagatct ctgcaaccga ctcttccatg tcctccattg
    27061 cattgcggat gatcaatttc aaataattta tgactgaaaa tcttcagaaa aataaagtta
    27121 actattggaa ttatacagga caaatccggt acaattccgg gttattgtac tccaggccgg
    27181 aagctaaaaa caaaacgaga atccagcaat aatgattggg agcgcgtgca gctagactat
    27241 tttcaatgca ttatgctttg ggtggtttgc tgcatgttat atatcagcac acctgccgat
    27301 cagcacatta atcattaatt cgtacctttg caaaaaattt caaatgagta ctttacaaac
    27361 cgaagcaacg atgaattaca aagtgaaaga tatcgcgctg gccgattggg gtcgcaagga
    27421 aattgaaatc gcagaaaaag aaatgccggg attgatggcg ctgcgtaaaa aatacggaac
    27481 tgaaaaacct ctgaaaggcg cccgcattat gggatcgctt catatgacca ttcaaactgc
    27541 cgtactcatc gaaacactca aagagctggg tgctgatgtg cgttggtcca gttgcaatat
    27601 tttttcaaca caggatcatg ctgccgcagc cattgctgca agcggaacgc ccgtgtttgc
    27661 ctggaaaggc gaaacccttg acgaatactg gtggactaca tcacaggcat tggattttaa
    27721 tggaaaaggt cctcacctcg ttgtggatga tggtggtgat gcaaccctga tgattcacaa
    27781 aggattcgaa attgaaaaaa atcctgcctt actggatact cctgttactg ctgccgatga
    27841 aaaagcattg tataaaatgc tgaaagaaat atatgttgaa aacccaaccc gctggaccaa
    27901 tctggtaaaa gaatggaaag gcgtttcgga agaaaccacc accggcgttc atcgtttgta
    27961 tcagatgatg gaaagaggcg aattgcttgt tcctgccatt aatgtgaatg acagtgtaac
    28021 caaatcgaaa ttcgacaatt tgtatggttg ccgtgaatca ttggccgatg gcatcaaacg
    28081 cgccaccgat gtgatgattg ccggaaaagt tgttgttgtt tgtggttacg gtgatgttgg
    28141 caaaggctgc gcccactcaa tgcgctcgta cggtgcccgt gttattacta cagaaattga
    28201 tcccatctgc gcgctgcagg ctgccatgga aggttttgaa attacaacgg ttgaagacgc
    28261 tctgccggaa ggaaacattt atgtaaccac caccggaaac cgcgacgtca ttaccattga
    28321 acatatggcc aaaatgaagg atgaatccat tgtttgcaac atcggacatt tcgataatga
    28381 aattcaggtg gaacaactgg aaaattatcc gggtatcaaa aaaatcacta tcaaaccaca
    28441 ggttgataaa tatattttcc ccgacggaca ttgcatatac atgctggctg aaggccgtct
    28501 agtgaacctt ggctgtgcaa ccggtcatcc gtcgtttgta atgagcaatt catttacaaa
    28561 ccagactctg gctcagattg aactctggaa caacaactat aaaacagatg tttatcgtct
    28621 tccaaaacga ttggatgaag aagtggcacg tctgcacctt ggacaacttg gagtgaaact
    28681 cacccagctt tcgcaagaac aagctgatta tattggtgtt cctaaagaag gaccatacaa
    28741 agctgagcat taccgctatt aaaatgctgt gcgaagattg gatacttcgc acaataatat
    28801 taaaaaagag cgaaggtcaa ccttcgttct tttttattgc acccgcacgt acaccacact
    28861 ctgcttatca cgtgaattgc tgttgataat aagtattcca agcgccggat ttttcaatat
    28921 gcttttatca acacaaatat ccagggtggt cacatttccc ggaataataa catttccacc
    28981 aagcttggct tgcaaccagg gctgcgctga tttcatggaa ataatttcca gaatttcatc
    29041 acccgtattg gttatggtta tctgaatgca cgtattgttt tctccggaca aagatatttt
    29101 tatatcgttc gaggacaggt tcaggagcgg tccggaaagt tttcttgttt tttgtccggc
    29161 ataatccgac tcatagctaa caatctggat acgtcgttcg cgtgcagcag aaatactgta
    29221 cacagagttt cgcgtatcat tggggttatc acttacaaac gggtctgaga gcgcttcacc
    29281 aaccggttct gccagaataa tcaaacggcc tccattggac gctgatgcat tgatataagg
    29341 ttttatctta cctccatcaa ttttttcaaa ataattgaca atactgctga ttctgcgttt
    29401 tgccagatag agattgtatt ctgttcctgt caatggactg cagaaaccct gaaccttcat
    29461 tgtaacaaca cttcctgctt cgagatcttc catcatcaaa ggagtaagat ccagcagttt
    29521 tttaaatcca ttggttacat aagccagaaa aaaaacttcg atttctgctt cagcattgta
    29581 ggcatcatca gcactgagac ccttggcata ttccttttta tagcgttcaa tcattgccat
    29641 ataggaataa tatgtttcat cgtatgttga caacgttacc gtatcacggc tgcgcggatc
    29701 aggaacatca ttatgaaaat acaaatcaag aggaagcaaa tcttcgatcc ggtctttgat
    29761 ttcattaatc acaataagag tatccatcgg ctctggcaat gtatcagctt ttattttttt
    29821 ttgtccgtaa taggcataca agtcattaca gcaggtttcg cctttaaaaa aataagatcc
    29881 ttcacgattg gatgtaaaat atccatcccg atcgaccgaa ttgatattaa aataaaaatc
    29941 attggctgga gaacaaatag gaattccagc atttacgggc tttttccaat cgctcaacga
    30001 accagcagaa taaaaaacat catatccacc caacccataa taccaatcgc tcgaaaaata
    30061 caatctgccg gattccatat caaaaaaggg tgttatttca ttacccggac tattcacaat
    30121 gcttcccaga ttggttggaa cattgtattt cccatctttg cgaacactga accaaatatc
    30181 aagctgaccc acaccacctg gacgatcgct gacaaagtac aatacttcgt tatcggcact
    30241 gtcaacgaaa aaaaagggtt gagttgatgt atattgatca ttatttattg aaatcttctg
    30301 cggataagtc catcgtccat tttctaattg agcattataa atgtcgcagc ggtatttata
    30361 ttcacctgac atttcgcaac gattgaacca cacttcggaa gcatcttcat taaaacagat
    30421 gtttgccgta tgtttcactt tattgtttaa tggttctgca accttgaccg ggcgcgataa
    30481 tccacgactt gtaaaatcac ttacgtaaag agatgacaaa aaagaattgc ccaataagcc
    30541 attttctccc tcactcagca atggctgcac agaagtgaaa tagagttttt tttcgccctg
    30601 ctgaaatcca ccaaattcgc tgaaaggaga atttaccggc agcgacaaat gctccaccga
    30661 tacttccaaa gaagtatccg cacgaatttt atcaatttca tcaaaataat tcagttgttc
    30721 ttcagctttt tcttcaaacc atgcatcatt gtttgtttgt tccagataaa gcgaataata
    30781 cgcttttgac ataagataat ccgacagatt acgcgacagg tcggccatcc agaacaaggc
    30841 ctgaggaaaa ttcttcccaa ggtctttttc atatacaata cggtagcatt gaaaagccat
    30901 attgtaatta aaaaactcac gtgaagattc gcccatttta taccataaag caatgtttga
    30961 tgtatcggtt ctggcagctt cgttataata attccatgct gcctgaaaat ttccggtttt
    31021 aaactctgca tcaccgcgca attcaaattc agactgagct tgaatctgca aaaaagcaaa
    31081 aaacagaaaa tatatcgtgt aaaaatgttt aatcataatc agaaaatcgg acaaggtatt
    31141 tcgcgggtag ttttatggtc ggctttattg aaaaccgcta caatgctaaa ttcaaaacct
    31201 ccgcgaaaac ggcttgcagg cgtaagtttc gaagtattca aatcataact gattccaaat
    31261 ttataattca gataatccaa tcgcgccgaa aaaataaaac catcaccaac cctgaaaaac
    31321 aaaccaccag aatatgcttt aaatgaccag gggtcgaaac tttttacaaa acgataatca
    31381 gcgccaataa tcaattccgt gaatgttcct tgtttggaaa ataacgcaga aggacacaaa
    31441 aaactgtttc tggtaaacgc atttttatac atcaaatctg caacccaacg cgtgcgcaat
    31501 ctgatagaat tgtcattaaa atgcgactgc atgggtctgt tgatgttaag catggaaagg
    31561 cctgcaacaa gttcatcctg agagcccatc cgcatcatat aagcaccgcc ggcatttatt
    31621 acgggatacc aaaaattgaa atccgaaaaa gtttctccgc cacccagcga cggatcatag
    31681 tgtaaaccat tatactgact gtcaaaagta agctgatcag cattaaaact acgctgatgc
    31741 atccgaaacg aaacacctgc tgaaaaaaaa cggttattga aacgattcaa cgctttgata
    31801 tacgacacag acaaaccaaa gccactggtt ccaaaagccg catcaccagc tttctcacgc
    31861 ataaaatcaa caccaatacc cactttaccc aaatgatttc cctttcgctg aagactcatg
    31921 tcgcccgctg caagataagt ctggtaaggc aaagtaactg acttccactg caattttgta
    31981 ttggccgaca aacgaaattg ttcgctcatc aggccggctt tggcaggatt aagataaaac
    32041 ggaacattgg aatattgact gaaatgaatg tcctgtgccg ttccgaaaaa cgacaaacaa
    32101 acaagcattg caaacaaaac aattctccgc atatgattac ctcaacaatg taatatttcc
    32161 ttttttaata aattgtgctt tgttgaaaca atcaatttca agataataat caaacacggc
    32221 aggttcgagc agctggcctc tgaatgtgcc atcccaaccc tcaagcggat catgggtttc
    32281 aaacactttt tcaccccatc ggtcaaaaat cacaaaatac atgttttcga tatatctgct
    32341 gcggatatat aaaatatcgt tatttccatc accattgggc gtaaatgaat tcggaatgaa
    32401 aatataggga tcataacaaa aaacatcaag aacaacgatg cgaagcgtat cagaataaat
    32461 acaaccattg ccatcatcaa acacaacggt ataggttgtt gtttcggtag gcgaagcatc
    32521 ggttacagaa ctataggcat cggcaagacc gcttgcaggt gtccacatat aatcaacccc
    32581 cggaacagat gaagcatgaa tagtggttga ctgtccctga taaatagtat cctgttccgt
    32641 ccaaacatca acgctatcat aaatataatc aacaataata tcaaatgtat tctgaaattt
    32701 acagtcgtgc gaatccgtaa tggtcaaaga ataatttcca tagcacaacc cggtcagatc
    32761 ttccgtcgat tgcccattgc tccattgata gacatagggg cctgttcctc ctgttgttgt
    32821 tccgtaaaca aatccattgc aagcaacatc acaattaacc ggacctgttt cagaaacata
    32881 tccgagcaac tgtggctgac ttatcatcac atttgacgac tgcacacaac cattaacatc
    32941 atacactgta acaacatata aacccgaaac gagattattg agattctgag tatgatcacc
    33001 agtactccag ttgtaggtaa aaggcgccac tccaccagaa ggattcaatg agactgaacc
    33061 atttgaatcg ccatagcaca acggctgagt aattgaaaca tttaaatcca tttcctgata
    33121 atcaacaaag acttcatctg tttccgtaca ataaccatta cttacctgaa gataatacgt
    33181 gtgcggcgtt gtatgaatgg ttgtacaggt acttcctgaa ggatacacat tgagtgtatc
    33241 ggaaaaattt gcatccgatg accaatgaac ggtaaaatca gtacctcccg aaaatgaagc
    33301 agtaagcgtt attcctggtg aacaggtcga agtatcattt ccggcatcaa cgatcaaatc
    33361 atacacattt actgtttgat aaagcgtatc gaaacaaaca ccatttgaaa taacaagata
    33421 atactgcgtt gttatgggcg gattagccgt tggattggca atggcgggat tactcaaatt
    33481 atttgaagga taccactgac aagtgattgt cggatccgaa aaaggaagaa ttccgatctg
    33541 cgtcattcct cctgagcaaa tgcccactgc ggcaagagta tctgtactgc tggataaaac
    33601 cgtaatttgt tttgtaatag tgtcggcact gttgcatgct gtagcatcag acacaatcag
    33661 cgttacatca tatgttcccg acacaggata atcatacgaa ggattacaat tggtgctggt
    33721 tactccattc ccaaaatccc aaaaacaggt tgccaggccg ggattcggaa aataagaggt
    33781 attattgaaa atcaccgtat aaggcaaaca aataacagga ggcatattga aatcggctac
    33841 aatggccgga agactgaaat caatttttac aactccattg ttgcaattgg ttgaattatt
    33901 tgtatttgac caggctcctg ttgttgtcgg aaaatcatca tatccgccgc aaccggcaca
    33961 aacagactga taaatacgtc ccatgcgatc aaatctgctg gttcctccat caacatgttc
    34021 ggcacttgaa ccaccgtaaa atgagccaaa aacaatgctg gaagcatcgt ctcttataca
    34081 cagaaaataa taatcattat tatcggttgt agtttgaaaa gcattagccg taatcggcaa
    34141 tccggcagtg ccaccaaaac cactcaaaag cggactaccc catgccgaca aataaatgtt
    34201 atgacaataa tcgaccaaaa aagcagtagg actgacattt actacaccat caccattacc
    34261 aaaccgtgtt gaccagataa tggtattgag cggcggattg attttagaaa taaactgtcc
    34321 acctccaatg gtattccatg ccgcattctg aatcagaact gttccggtag aagcgctttg
    34381 tccaagcaaa tatacattgt tttgtttatc agtttcgaca aaataaacct gatcataagc
    34441 cgatgttccc caataggaag acctcagcac ggctgatcca tcgctttgaa taagcgaaac
    34501 aaatccatcg gacggccctc cctgataaac cggcataaca gcattggccg ataccggaaa
    34561 attagtactt gaagttccac cagccacaga aagcttaccc aattcattga gcgaaatact
    34621 ataaatggca tcagcagatg accctccgag ataagttgac caaatcattg ttgttaacaa
    34681 acgatcacat ttcaaaataa aaccatcctg actaccgcct ccatacatgg tttgatgcac
    34741 accataagtg acaggaaaat ttaccgaagc cgtacttcca acaacaaaaa cattgctgtt
    34801 agcgtcgagc agaatttcac cacggacatc atcggcataa ttatgtttta aaaccgcatc
    34861 catattgagt ccatcgttga gacttccgcc aatataggtt gaagcaatca atgcagtccc
    34921 agacgaatta aggcaggtaa cacccagatc gcatccattg gtaaaattaa tgacataggt
    34981 aagaacataa ggagatccgc cattgaaagt actgtcaata catccgcttg tagtaggaaa
    35041 atcagccgat ccggtagtcg caaaaatata taattcatca gccgaattca ccacaatact
    35101 attgggaaca tcgggcgaag agccacccag atatgtactg taaatcaagg ttgttccatc
    35161 ggtattgtat ttggttactg ccacatcaac attaccacca taagtaacct gataagaacc
    35221 cagcgttgtg ggataaccct gaccaaaaac aattccgcca gcataaacat aacccagttg
    35281 atcgtaagta gcagtatagc cccagttatc agcagtagaa cccgaatatg taccaaaaac
    35341 aacaatcggg tcgatgatta gctcataatt gcgattataa ttaccacccg gttcaaaacc
    35401 cagtgtttta ttttttaaaa cataatcgca tcggacaaaa acagtgtcgc catcaatcat
    35461 ttgccaagcc agaggccgca gctcggttac acttcccagc gaagtaccaa aaataagatt
    35521 tccgttttca atccggatat tatccacccc attatactta atacgaatat cattaacgct
    35581 ggcattggga cttacagcaa attcatattt caaactgccg ttttctacac ccaccgataa
    35641 atcgattcct tgatacaggt ttttgtattt tactttttcg aacccatgaa catttgattt
    35701 ccaacgagac ttatcgtttc cgagataata attataataa ggttctatct gactttctgc
    35761 gcgcatttcg ggatacaatg caccaataaa ctccatttca tagcaatggg cattaatgat
    35821 attatccggt tgttttcgta actttttttc ttcaggagaa agcattttga accgcatcaa
    35881 ctcagccacc atagctttat cacgtaaatc aaccactaag cgattatcat aaagaaacaa
    35941 ctggcccgta ggcaaatccg ctttataagc aaatgattgc tcccattgac ccatattttc
    36001 aacaaaaata atgtgccctc ccagggtatc cggatcttta tggtttacac cggcaaacga
    36061 aaggatactg gccattaata taatgcagag agacaatatt aattggcgca tgataagttt
    36121 ttctaacaaa gataagaatt tttcgacaga aatcaagttt ttgtcaggca gaattgaagt
    36181 tttgtaaata aatatccaat atctttgttg aagaaattgt acatttttat gaaaaatatt
    36241 ttgtttgtaa tatccggggt tttttgtctt ttttcaaatc catccatttc gcaaatcgtt
    36301 gaacccacaa taataacagc acttccaacc tgtctgcatg aatcatccgg aattgaagct
    36361 gaaaacacaa acagcatatg gacacataac gacagtggcg attcggcccg catttatcaa
    36421 atagacacac ttggaaacat tctccgaaac atctgttttc aggtggattc agctattgac
    36481 tgtgaagaat cgacccgtga tactaacgga aattattatc tcggcgactt tggaaacaat
    36541 tccaacgacc gcaccaatct tcgcatttat aaaattacaa atcccgattc gataacaaca
    36601 agtacaataa taccagaatt aataaccttt cgttttgaag atcaggagct atttccaccc
    36661 gattcggccc atatggattt tgattgtgaa gccatgtttc attttcagga ttcactctat
    36721 ttattttcga aaaacagaag cggaaacatg ttttgtaaaa tgtaccgctt gcccgatgtc
    36781 gccggtgatt acattgccga actggttgac agtttcaata caggaacatg gattacatcg
    36841 gccgatatca atgcggcagg caacaaaatg gtactgcttt ccgaatcgcg gctgtggctc
    36901 ttttccgata ttcaaggcac aaactttttt gatggaaatg cacaaatgtt caacatgtat
    36961 ttttcacaaa aagaagcagt ggtttttgtt gatgattcta ctctttatct cacggatgaa
    37021 tatcttttcg gaaccggcgg aaatctatat cggattgatt tgaattcggt gataaacgga
    37081 acagataatc aacagaaaaa acaactgacg atatttccaa atccggtttc aaatgcgttt
    37141 ttcatcaatg tatcagaaat aaatgctgat tttgttgtta tggtattcga tgctacagga
    37201 agaacagttc aatcatgtaa aagctgctca gaaattgata tttcatcatt gtgcgaagga
    37261 cattatttag tgagagtgat ttctgaaaat caatgtcttt caggcaggtt tgagaaattc
    37321 tgaatctgtt tatacttgtt gtggctgtaa tgtcatttct actcccgtga cttcgtcccg
    37381 ggctaatatc tgcagcgctg gattttattc cgcggaaggc ggaagcacta tttgtactac
    37441 atagctgttt tgacggcttc acgtaaagcc atgtgaaacg tggcaagctg tgacatagtc
    37501 gccgctcaac cttaacctgg aatattattt cggagcccac ttcaccaaca accaggcgat
    37561 accggcaaaa acaatatttg gaatccatac cgccagcatt ggaggcagat ttccattggt
    37621 cgcaaaagta ctactgactt gcataaataa aataaagcca aaactcagcg ccaaaccaat
    37681 acctatatgc aatcctgttc cacctcgtgt tttacggctc gacacggcaa ccccgataat
    37741 agtaagaata accgtagcaa aaggaaaagc aatccgccgg tgccgctcta cctcaagttg
    37801 ttttatatta tcagcgcccc gcattttctg tctggctata tgatcgttaa gttcaaaaaa
    37861 atccataaca tccacatttt ccatatcctt gttgaaatcc ttaggaagaa tagcaagcga
    37921 agtatcaagt gttggcaaat aacgtaattt ttcaacagaa tccttcatcc gtcggtcata
    37981 tacttcggtc atttgccatt gaccgcttat ggtatcataa attgccatat tagccgataa
    38041 ttttcttgac ataccatttt caaaatcaat ggtttcaaga gtaaacatca tggcacgatt
    38101 cgatttgaaa ttaaatgact ccatataaac gtattcaccc ggtgccatct gcatatgaat
    38161 attgcgagca cccattggtt tgttttttcg gatgaaatta tactcaaatt cgagacgaac
    38221 cgcattggcg ggaggtatca gaaaattctg aagaacaaaa gaaaaagccg ccagaaacac
    38281 tccggtaaaa acaaaaggca tcaataaacg accaaaactt attcctccgg taagaatggc
    38341 tacaatctct gtgttgcctg ccattttcga tgttacaaaa acaacagcaa taaatacaaa
    38401 caacgggctg aaaagattga taaaatatgg aataaagttg aaataatatt taaataaaat
    38461 atcgcggaat gttgcatgac gatcaatgaa ttcatcaatt ttttccgaga catcaaaagc
    38521 aataataatg agcagcaaca atgcaattgc aagcacaaac gaagataaaa atctctttac
    38581 aatatactta tccagtattt tcatcagaga cgggtcatgg tttgtttttc agcttcagca
    38641 gcccagattt cgtaatttcc ttccatcacg gcctgtcgtg cttcggccac caaacgcaga
    38701 taaaaagcca gattgtgaat acttgcaaga atgggtccaa gcatttcgcc ggctacaaac
    38761 aaatggcgga gataggattt ggttgtggtt ttatcaattt ctaacgggct gcattcatcg
    38821 atgggagaaa aatcattttc ccatttttta ttgcgcatat tcagaattcc atgacgtgtg
    38881 aaaagcatcg cattgcgtcc attacgcgtt ggcatcacac aatcaaacat atctattcca
    38941 tgactgattc cacgaataat gttagccgga gttccaaccc ccatcaaata acggggtttg
    39001 ttttgcggca gaatatcaca aacctgttcg gtgattttat acatggtttc ggtcggctca
    39061 cccacgctca atccaccaat ggcatatccc gggctgtcga actgactgat gaattttgcc
    39121 gactgttcac gcaggtcttc atagacactc ccctgcacta tcggaaaaag gccttgatta
    39181 tgcccgtaga ggggctttgt tttatgaaaa tgattgtagc atcgttcaag ccattgatgt
    39241 gtcaactgca tgcttttctg agcatatttc cgatcggccg gataaggcgt acattcgtca
    39301 aaggccatca taatgtcggc gccaatgaca cgctgcatat ccattacatt ctccggcgtg
    39361 aactgatgcc ggctgccatc aatatgtgat tgaaaaacaa caccgtcttc ggttatctta
    39421 cgtattttcg aaagcgaata aacctgataa ccaccgctat cagttaataa aggacgatcc
    39481 catccattaa aaccatgcag acccccggcc tgttgtaaaa ttccggcacc aggtctgaga
    39541 tacaaatgat aggtatttcc aagaataatc tgcgcatgaa tatcgttgcg caatacatca
    39601 aaggtaacac cttttacagc gcccagtgta ccaaccggca tgaatatcgg cgttagaata
    39661 tcgccgtgat cggtggtcag tatgcccgcg cgggcttttc cgcttgtatg ctgaatcgaa
    39721 aagttcatag aatttgaaag ttcaaaaata cgataataac gggaaatgat agtcctgagt
    39781 attttgaaca ttcattaaaa acttcagaaa caaaaatttt gaacattctc aatattagct
    39841 gaaaaacagt tcaaaattct gatagttaca tttttttgca ctctacccgt cagatgcaag
    39901 caatcggttc atttattttg gaaaactccg acgatttcat tgaataaacc attccgtacc
    39961 tgacggcacg
//
LOCUS       DAOPKE010000015        38337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1129_length_38337_cov_11.491275, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000015 DAOPKE010000000
VERSION     DAOPKE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_38337_cov_11.491275"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2189)
                     /locus_tag="PKY63_03500"
     CDS             complement(<1..2189)
                     /locus_tag="PKY63_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPE99711.1"
                     /translation="MKQLFLISMILMAALAARSQDLLCSWHFDTLKAAPNTELVIPAD
                     SGAQDNIAAIYADGTNGSSAWICAASNTEINSFSGSTLNDDRTTPLASSAIALINSAA
                     NGKGIVLKFSTTGYENPIISYAARGTGTGFNEHYLEYSTDGITFTRFDTTAANTSSTW
                     LTETIDLSSYNILDEAANVYIKVIFVGASSSSGNNRLDNFSITATSTSATDIIPPTVI
                     SATTTSLSDIWVRFSEPVGASAEVTANYTGVGAITSATRTTGLDSVHIVLTTPLTFGI
                     PDTIIVNNVEDIAGNPMAAAQSFPVQFGVLDVTPPTAISAWPETHAVVKVKFSEAMDD
                     VTAETVANYTGLAGISSATLNATMDTVTLALSTNLINAVADTLYIQNIEDTAGNAMTA
                     QQMFVLMLDTVTTTKALVITEIMYNPPSLDTLEYIEIYNNDIVPVDLNGYTLKYNTSA
                     YTFNSSLIMNPGDYVLVSPDANSCNIFYGQTFIQGNTTGISNTAAAIVILSPGGLLVD
                     TVRYAAASWPTEANGGGYSLTLCDPNTDNNDVTNWSLGHLPYGTIETTPVFANPGQGC
                     EIPDDLTPPVVDTAFATDLNTIKVVFNEVVDDVTAETVANYTGLAGIGTATLNVAMDT
                     VTLALSTPLIDNVADTVHIAGVADTAGNVMAASQSFRVIYSVNDIVPPTAIAAWAETH
                     SVVKVKFSEAMDDVTAETVANYTGLAGISSATLNATMDTVTLALSTNL"
     gene            2460..3740
                     /gene="glyA"
                     /locus_tag="PKY63_03505"
     CDS             2460..3740
                     /gene="glyA"
                     /locus_tag="PKY63_03505"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPE99712.1"
                     /translation="MKHDKQIFDLIEQEKARQMHGIELIASENFVSEQVLQAMGSVLT
                     NKYAEGYPGKRYYGGCQIVDQTEQLAIDRACKLFGAEFANVQPHSGAQANAAVFFACM
                     KPGDTFMGLDLAHGGHLSHGSPVNLSGINYKPVAYHVKEETGLVDYDEMEKLAKECKP
                     KMIVAGASAYSRDWDYKRMREIADSVGALLMADIAHPAGLIAAGLLNNPVEYCHIITT
                     TTHKTLRGPRGGMILIGKDFDNPWGITTPKGVVKKMSEVINSAVFPGQQGGPLEHVIA
                     AKAVAFGEALEPEYKEYMQQVRKNAAALADAFIKKGYHIISNGTDNHLMLIDLRQKFP
                     ELTGKKAENTLVLADITINKNMVPFDSRSPFQTSGIRVGTPAITTRGLKENHMAQIVD
                     LIDKVLSDPDNEAVIADVRKQVNAMMKDFPLYAW"
     gene            3765..4277
                     /locus_tag="PKY63_03510"
     CDS             3765..4277
                     /locus_tag="PKY63_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE99713.1"
                     /translation="MSSKTLLIMRHGKADGQVGSRNDIERPLTELGRKRTDLIARELI
                     KKKVLPQLIISSPALRTLQTSQQIAAGLNLSADAVQINEDLYFKTVNDYFDALYAAPD
                     EKNCIMIVGHNPLVTQFANFFLSQKVVDMPTSGLIALQSDARTWPEFVIAFKKALFYL
                     IPKNLENEKT"
     gene            4264..6336
                     /gene="ppk1"
                     /locus_tag="PKY63_03515"
     CDS             4264..6336
                     /gene="ppk1"
                     /locus_tag="PKY63_03515"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPE99714.1"
                     /translation="MKKRENDLAGLYVNRELSWLDFNDRVLQEAECADSPLLERIKFL
                     GIFSNNQDEFFRVRVATLKRIVKFRRKSEYELYRYFSPEKVLTEINQRIVGQQKRLAQ
                     VYNHLEKELAREGVHIINEKEVKYAEHKCFVRKYFDTVLRTYIFPIIMRNYNADTMLR
                     DKSIYLAVSMRNSARNTKPEYALLEVPSEQLSRFVVLPEMNGEQYVMYIDDIIRYCMP
                     DIFKSFDYDEFKAYTIKFTRDSELEMDSDISKSFLEIISDSLKQRKTGNPVRFVVDRD
                     MPEEMLEYFFDKIGLTRDDTLVSSGRYHNFKDLMNFPSLGKNHLSFEDREPILHTRLE
                     KVRSVFDEMKKKDILLSFPHHPFKYVIDFLREASIDPAVRSIKMTIYRIGKNSNVINA
                     LINAARNGKSVTVFMELQARFDEETNILWSTKLQEEGVRVIHSIPGLKVHTKLILVKR
                     VEGKKSVYYTNVGTGNFNEITASLYCDVSIMTADQTIGLETAQVFEMFEASYRSFSFK
                     QLVVSPMEMRPFIEGMIDKLTESALRGEDAYAAIKLNNIVDKDIADKIYAAAQAGVKF
                     DIICRSACVLQPINENLRIISIVGRNLEHSRILYFRCGQWERVYITSADWMKRNMDRR
                     IEVMCPVLDKDAAELLKKILFVHLQDNAKARFVNGKKNNIYIRNNGPLFNSQDEVYRL
                     IRSGDFEI"
     gene            6333..7211
                     /locus_tag="PKY63_03520"
     CDS             6333..7211
                     /locus_tag="PKY63_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99715.1"
                     /translation="MRYAAIDIGSNAVKCMVGTVIDSGYPVVIKEMYARVPVRLGTDV
                     FQSGAVSAESEKKLLDSLRAFVLLMKSWNISGAVIAATSAMRDASNGHDILKNIENET
                     GLRTWIIKGKIEAALITDLFAGLGEKKKQKMMIDLGGGSTEVTFANPDNKRKSKSFQI
                     GAVRSLMHEASRVELKKMLDFIENNIDPFGHLIIASGGNINALKSHFGLAEPMYLSFQ
                     ELQRAYDQLHPLTVSERVERFLIHPDRADVIVPAAEVFLKIMKFANAQKLMVPKAGLA
                     DALILMQHQGKTPEQY"
     gene            complement(7530..7811)
                     /locus_tag="PKY63_03525"
     CDS             complement(7530..7811)
                     /locus_tag="PKY63_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99716.1"
                     /translation="MKNFQNKNNNSEYFALPDAKAAAAARRCRHFDGVYPAVNTSTPL
                     SVTAGRAQCDSGGALDDSVILIYLLRFLRGKPRVDTSQRCFASPFSPQP"
     gene            7893..8735
                     /locus_tag="PKY63_03530"
     CDS             7893..8735
                     /locus_tag="PKY63_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPE99717.1"
                     /translation="MNNDKSLVVFDNKKVRRFYDEETEVWYFSVVDIVEALTDSVNPT
                     DYLKKLRKRDPELGTYLGTNCPQIEMLTNGKKRKTLAGNVKDIFRIIQSIPSPKAEPF
                     KQWLAKVGYERVQEMNDPSLSMDRARENWQKMGRSEKWIQQRMTGQETRNKLTDYWKN
                     AGVKEQDEFALLTNIIHKEWTDLTVKQHKQLKGLKSQNLRDHMSEAELIFTALAELST
                     RQIADSEQAKGLIENAKASKKGGAVAKNARKELETKTGKSVITGENFLPPAKEKKKLP
                     PKKK"
     gene            8866..>9635
                     /locus_tag="PKY63_03535"
     CDS             8866..>9635
                     /locus_tag="PKY63_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE99718.1"
                     /translation="MNSPRDLFFRHVAQTSPEPLALEIVSANGSKLFAADGMEYLDFI
                     SGISVSNLGHNHPEINAAVIEQVNKHTHLLVYGEFIQSAQTGLAELLCSVLPSSLNNV
                     YFVNSGAEAIDGAMKLAKRSTGRFEILSCRNAYHGSSTGPLSIMGSEEYKTAFRPLLP
                     GTGLIDFNNPDDLKCISDKTAAVVIEPFQAEAGVQIPDKDYFAALRKRCDETGTLLIL
                     DEVQTGFGRTGKMFAMEHFGIVPDILVLAKALGGGFPLG"
     assembly_gap    9636..9735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9810..10289
                     /locus_tag="PKY63_03540"
     CDS             9810..10289
                     /locus_tag="PKY63_03540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPE99719.1"
                     /translation="MINNPDRKFLQMAVDLAVENVRSGKGGPFGAVIVKDGEVLATGV
                     NLVTSNNDPTAHAEITAIRNATAKLKDFQLNGCTLYSSCEPCPMCLGAIYWARPERLV
                     YATSKHDAADAGFDDAFIYDELELPIAQRHLKTEYCPEEPYKLPFQIWKESAAKTEY"
     gene            10562..10903
                     /locus_tag="PKY63_03545"
     CDS             10562..10903
                     /locus_tag="PKY63_03545"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPE99720.1"
                     /translation="MEEFDRKERDEIYSRPVRAGKRTYFFDVKEMRSGEMYITITESK
                     KRFNEENGKFFYEKHKIFLYKEDFDKFAKGLDAVVNFIRTGEKAPEEDFHEFAGQNEF
                     GSEMNQEQSDL"
     gene            10993..11775
                     /locus_tag="PKY63_03550"
     CDS             10993..11775
                     /locus_tag="PKY63_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99721.1"
                     /translation="MGKVISIANQKGGVGKTTSAINVSAGLAVLENKVLLIDADPQAN
                     ATSGLGFDPKNIRTSIYECIVDSVNPADAILHTKTPNLDILPAHIDLVGAEIEMINME
                     NRERMMRKVVRQLKDQYDFIIIDCSPSLGLITVNSLTASDSVLIPVQCEYFALEGLGK
                     LLNTIKIVQTRLNQDLAIEGILLTMYDVRLRLSNQVVDEVRSHFKEMVFETIIPRNTR
                     LGEAPSFGESIIMFDAESKGSISYLNLAREILQRNNMIKQQA"
     gene            11809..12678
                     /locus_tag="PKY63_03555"
     CDS             11809..12678
                     /locus_tag="PKY63_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPE99722.1"
                     /translation="MNTRKNALGRGLGALLEGANDESAQGVSAPVIAFPMIDIDEIEA
                     NPYQPRDRFEQEALKGLSESIKHQGLIQPITVRKLKNGKYQLISGERRLRASKMAGLT
                     QIPAFIREADDEAVLQMALVENIQRENLNAIEVALTFDKLMDEYKMTQEMLSEKVGKK
                     RTTVANYLRLLKLPIEIQAGIRDNKITMGHARSLVGLEDEAKMRIVFDRIIAEDLSVR
                     QVEDIVRGMVAVPENKTAKKKEKDLAHEELQKSIAAKIGLKVQVKSGLKGGTLTIHFK
                     NEEDLKKIETIFH"
     gene            12681..13226
                     /locus_tag="PKY63_03560"
     CDS             12681..13226
                     /locus_tag="PKY63_03560"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPE99723.1"
                     /translation="MNRFAATILLIFAFLNIVKAQNIDDTTSVAHSPRKATIMSACLP
                     GLGQAYNHKYWKIPILYAGLGGAGYAIVWNNNKYQDFRKAYIARTDTIDSTTDNFPRY
                     SASNLLDLRNYYRHNLELSVIIITAVYILNIVDASVDAHLYDFDISDDLSLRIQPSVW
                     NVGPGTSSFGGGLSLTFKLKP"
     gene            13223..13951
                     /gene="dapB"
                     /locus_tag="PKY63_03565"
     CDS             13223..13951
                     /gene="dapB"
                     /locus_tag="PKY63_03565"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPE99724.1"
                     /translation="MKIFIIGYGKMGKMIESLAAKEGHEIVAVVDAGQFWPRFTDHDR
                     PDVAIEFTQPDEVSDNLKHCIEWGIPVVTGTTGWEDSREEIRNLCLQKNAAVMFGSNY
                     SIGVNVWFNALKAVAAGLATTSGYQVSIDETHHVNKKDKPSGTAITAANLIYERMQQY
                     DGWSLKDENNKIHIEAHRRQDVTGMHSVLFSSEMDEVELVHRAHSREGFAKGAIRAAE
                     WLCNKKGFFDFSTVFEEIFAFGNK"
     gene            13985..15583
                     /gene="lepB"
                     /locus_tag="PKY63_03570"
     CDS             13985..15583
                     /gene="lepB"
                     /locus_tag="PKY63_03570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPE99725.1"
                     /translation="MDLVIIFTLVFFLLTLVSLAFIFRKAGQSPFLAFVPVYNIIIWL
                     RIVKKPLWWLIFTIMPYINVFMVMLLIVETLKAFNKHSIGAQVLSIMFPFFYLPYLAF
                     NKNLTYIHPDQQEKRKKTFSREWVEAIVFAVIAATVIRMFFIEAYTIPTSSMEKSLLV
                     GDFLFVDKMTYGSRTPMTPFSFPFVHHTLPLTKESKSYISWIQLPYYRYPAFSKIENN
                     DVVVFNFPAGDTVMLKNQAKTYYGMVIEQAVGMAQEAGKDLKDYPIYMNLARSAVLQW
                     SDITVRPVDKRENYIKRCVAIPGDVIEIKERQLFINGKEVENPEQMEYNYKFTLKPGS
                     IISRKKLMNLGLSLEDIENSKQTAAYYGYSSDVMILPMTFDMLRDFNNQFGTLLDTVP
                     EVMNETAWHPSIFPHSPAFPWNRDNFGPLEVPMKGQTVKIDTTNYVLYSRIISIYEGN
                     TLEVKNGKVFINGTVADTYTFKMDYYFMMGDNRHNSADSRYWGFVPEDHIVGKAAFVW
                     LSLDKDRSVSNGKIRWRKMLRLVH"
     gene            15905..18265
                     /locus_tag="PKY63_03575"
     CDS             15905..18265
                     /locus_tag="PKY63_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPE99726.1"
                     /translation="MVPSFINNLNDVQRDAATCTEGPVMVLAGAGSGKTRVLTYRVAY
                     LIQSGVQPYNILALTFTNKAAREMKERIFQLVGDAKANALWMGTFHSIFARILKMESE
                     KIGYPKDFTIYDTDDSKSVVKAIVREFQMDDKAYNPGYVLNRISAAKMNLINPSQYKD
                     DPEIQYQDKMAKKPLITEIYAEYWNRCKRSSAMDFDDLLFNTNLLLHHYPEVLKKYQD
                     RFEYILVDEYQDTNYAQYLILKRLAALRENICVVGDDAQSIYGFRGANIQNILNFNKD
                     YPSLKTFKLEQNYRSTKTIVAAANSVIQNNQSQLHKMVWTGNDEGEPISLIKASNDID
                     EALLIANSIFHTRMTYHLKNSDFAVLYRTNAQSRSMEDALRKLNIPYRVYGGMSFYKR
                     KEVKDLIAYFRIVINPNDNEAFYRIVNYPARGIGNTTLHKISVHASEQEVPVWAILQN
                     IDIYHHDFNASTIKRLKDFMELIRHYQQQLLTSTAWELATKIATGSGLVKELFEDRSP
                     EGIARYENIESVLNGIRSFEDEYEQANDGEKPSLSDFLQMVSLLTTVDEADDKENTDR
                     VSLMTVHAAKGLEFPYVFVAGLEENLFPSSMSMSSKAEIEEERRLFYVAVTRAMQKLT
                     LSYADTRLKWGELNFCTPSRFLEELDSSLLDIPQKFSPGLKQKDDSSFRMKLQDASSE
                     NNFKSKYQKGAGYSFNKKVSDKSGPEFKKKEVPQPKSAPAFNDSELADVDDISEGMRV
                     EHARFGVGTVLQIEGNGQNRKALVEFDLSGQKQLVLRFARLKTIQN"
     gene            18333..18965
                     /locus_tag="PKY63_03580"
     CDS             18333..18965
                     /locus_tag="PKY63_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPE99727.1"
                     /translation="MEYNTQREHLEMTEYGRYVKQMVEFIKTMPGKESRTKAANAIVH
                     AMMTVQPGPRDMIDYKRKLWDHLMLMSDFKLDVDSPYPLPEPNKKEIAQFLNYSVPYE
                     VRFRFYGRYVEKMVKKAVEMEEGEDKTELVRLIANTMKKLYLTWNKDSVKDEIIIEQL
                     NILSEGRLALDPGYVLQDTTEFVKNNKTQQKIWTQQSKKNKKNKYRKLKQ"
     gene            18968..20272
                     /gene="murA"
                     /locus_tag="PKY63_03585"
     CDS             18968..20272
                     /gene="murA"
                     /locus_tag="PKY63_03585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPE99728.1"
                     /translation="MSSFEITGGNRLSGTITPQGAKNEALQVICAVLLTDEKICISNV
                     PDIRDVNKLIDVMRFLGVDVSNPSTGTWEFKADNVQIDYLQTDEFRQNAGVLRGSAMI
                     LGPMLSRFGVGAIPRPGGDKIGRRRMDTHFIGLEKLGATFEDGDPTFYIARRKGRLKG
                     AFMLLDEASVTGTANIVMAAVLAEGTTTIYNAACEPYLQQLCNMLVRMGANIEGIGSN
                     LLTIHGVEKLNGTEHTLLPDMIEVGSFIGLAAMTQSDIIIKNVSVKDLGLIPDVFRRL
                     GITVEQQGDNLHVPSIDVYEVETFRDGSIMTIADAPWPGFTPDLLSVVLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATIIGLERKTQLRGIEMTSPDIRAGA
                     SLLIAALSAKGKSIIHNIEQIDRGYQNLDGRLRALGAEIRRV"
     gene            complement(20379..21254)
                     /locus_tag="PKY63_03590"
     CDS             complement(20379..21254)
                     /locus_tag="PKY63_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPE99729.1"
                     /translation="MAKRINTQTVRGQQAVDGAGVHLRRVLGLRTITDFDPFLMLDGF
                     DSTNPQDYIKGFPWHPHRGIETVTYLIQGEVEHGDSLGNKGVIRNLECQWMTAGSGII
                     HQEMLQASDRMLGCQLWVNLPQKDKMCAPAYNDIQQKDVPLIKEEHATVRVLSGMYKD
                     MAGALKSHYVKVQYIDVALNAGSHWIYNETPDNETLFLYLIDGTLAADETLHKFEDKA
                     CAMLMKAASEQRNTYSDVVVKAGPDGARFLLIAAEPLHEPVAWGGPIVMNTQEELNLA
                     FEELDEGTFIRHVRS"
     gene            complement(21401..22138)
                     /locus_tag="PKY63_03595"
     CDS             complement(21401..22138)
                     /locus_tag="PKY63_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99730.1"
                     /translation="MKSIVSVFLLLICVLSSTGQTQIAEYKIFPVDETGKDTSLVTFV
                     AQLKNILTEKDTARLFAIMHPKMVTSFGGIMFGKQDFMSQWHLENAKESEVWDLMLHA
                     INPGGVFCKGIFDEDTIEAAIQYPYASDGRLYQSAAAHFPDMYFDPYFTGVCIRKNAP
                     VYESCDESSKQVATLSYDILLIDYEATMNAQPDAGFDFKMHYINTLDGAVKGWIKTSD
                     IYALGGRTLFLEKKDGKWNITGFVAFD"
     gene            complement(22310..24550)
                     /locus_tag="PKY63_03600"
     CDS             complement(22310..24550)
                     /locus_tag="PKY63_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPE99731.1"
                     /translation="MMFSFLAFVLLLFLININFLWLFGSSPRISDIKNPQQSIASEIY
                     SDDGKMLGRFYRENRTPVEYDELSPLLVETLIATEDERYYSHSGIDFIGLFSAVKDWF
                     SGNYRGASTISQQLIKNMYKTRSTYSKGLCGHIPYLRVVVMKLKEWIGAFKIEMYYSK
                     EEVLAMYLNTVDFGNNTFGIKTASRVYFHTTPKELKAEQIAMLIGMLKAPSTYNPLKN
                     PKNCTKRRNVVLNIMQNHKLISITQNDSLSALPLGLKYKVEDVSDGEAPYFRNAVAAS
                     IGTWCRTHGYDIYSSGLKIYTTLDSRIQTYAEEAVNKQMTFLQREFERHWGNENPWRD
                     INYNEIPDFIYNNALECPRYKQYRKEAGSEDSAMTLMKQPIAMKIYDPKLGVVDTTMS
                     PWDSISYTKRLLHIGFVAIEPQNRYVRAWVGDLNYQLFKYDHVSQAQRQPGSTFKLFV
                     YTAAMAAGMAPCDTRRDQFIEWKYKENGKDMVWRPHNVTGKHFGIPVTLRYAFAHSIN
                     SIAVAVAREVGLDKVMEYAYRLGIRSKLEKVPSMCLGSSDVKLLDLVNSYATIIADGA
                     YEAPVLVTRIEDRDGNIIWQHKPHRKKVVDYETAVMMQEMLKAGMKESGSTTQALRKY
                     DLFRYETDFGGKTGTSSNFSDGWFVGVTHKLVGGAWVGGEQRCIHFRNGFELGEGGYS
                     ALPVFGIFMQKVIKDESLKKYRAKFPEPKKKLTKKYECWTSIPDSLMGDSTEMHGVDS
                     LFNTGL"
     gene            complement(24765..>27499)
                     /locus_tag="PKY63_03605"
     CDS             complement(24765..>27499)
                     /locus_tag="PKY63_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99732.1"
                     /translation="PYLDWGVVNGITYYDVELDTTASFSSPLHQLVPQSSSYSYYYPS
                     NLRFGQKYYWRVRARHSTDTTSWSATWNFTTMDNVYLVAPSNGATGQDVNPYLDWGVV
                     NGITYYDVELDTTASFSSPLHQLVPQSSSYSYYYPSNLRFGQKYYWRVRARHASDTTS
                     WSATWNFTTHNTMTLTSPSNGAINQNVDVTLDYGAVSGITHYDVEYDTTASFNSPLHQ
                     VKTTLTGYSSYTPGNMLFGTTYYWRARSRHSADTSTWSAIWSFTTHNTMTLTSPSNGA
                     INQNVDVTLDYGAVSGITHYDVEYDTTASFNSPLHQVKTTLTGYSSYTPGNMLFGTTY
                     YWRARSRHSADTSTWSAIWSFTTMNSMALTSPANGATAQPVNTTLDWAAINGITHYDV
                     EYDTTASFNSPLHQVQTTLSGYSSYTPGNILFSTTYYWRARSRHSADTSIWSSVWSFT
                     TTDGKPVHVSPANAATGVSLNPTIDWSVVSGITLYQYEYSADPTFSSATPTGTGTTSQ
                     AGLSNLSYGQTYYWRVRTCHPADTSDWSTPWSFTTLYQLTTPVVLSSPADGTTGIATS
                     TVNMTWQTYATAVFYEYMYADNASFNNAVSGSAVTTSQVTAPLLPGTTYYWKVRANNG
                     SGYSPWSTAWTFATSGLAVPVLISPANGATDQNLSLTLDWQDAAAATFYEYELDDDVM
                     FGSPIASGSPTISNAAVSSLAYNTTYYWRVRSSDGTNFSAWCSPWSFSTATLTAPTLI
                     TPANGASNQAVTNLLLDWSDISGASMYEYYYDTDINFTNPTHAIVTVSQVTITGLSNN
                     TSYFWKVRSSDGAIYSPYSAIWSFTTETGVGIEENETNILLYPNPANENATLQFGNAA
                     ERTIRVISPTGQVVREISTNEAHFNLDLSGMNSGLYMIIIVESKEMKTLRLTIE"
     assembly_gap    27500..27599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27600..28327)
                     /locus_tag="PKY63_03610"
     CDS             complement(<27600..28327)
                     /locus_tag="PKY63_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99733.1"
                     /translation="MKKLLLAAVAALLLHAPSFALLVTPSMVAPSHGSVGQTVNVLLD
                     WGTSTGATYYQYKLSTSPTLAGATANSTGSNSYFYTSELKFNTTYYWSVRARSATDSS
                     DWSVIYMFTTMDGVSLVSPSNGATGQDVNPYLDWSIVDGITYYDVEIDTTASFSSPLH
                     QLVSQSSSYSYYYPANLRFGQKYYWRMRARHNVDTTTWSTTWNFTTMDNVYLVAPSNG
                     ATGQDVNPYLDWGVVNGITYYDVEL"
     gene            complement(28349..28726)
                     /locus_tag="PKY63_03615"
     CDS             complement(28349..28726)
                     /locus_tag="PKY63_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99734.1"
                     /translation="MKNFFRIFALLLFVLTVSGANAQNTATRDSEPTEQSPQAVFSIK
                     VLFHCANGKALLETELPKKRGVISAIAHLDTKIVDITYNTKMTGPDAIKEYIHQIGYL
                     CEGDPADTKLKHKACSHGENHEE"
     gene            28926..30227
                     /locus_tag="PKY63_03620"
     CDS             28926..30227
                     /locus_tag="PKY63_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HPE99735.1"
                     /translation="MKKIIAIRHENKYEMERRVPIIPDHVGSLINKGYEIHVQSSAKR
                     VFSDDEYRNAGAIVNNDISDADIIFGVKEIPMQDLMPGKTYMFFSHVIKGQPYNMPML
                     KRLMELKCNLIDYEKVTDEFNKRVIFFGKYAGLAGMINTLWSVGQRYQKLGIETPFVK
                     LRQSYTYNSLSEAKKAISKVGYEIMKHGLPKELQPFTIGITGYGNVSMGAQEILHLLP
                     VKEIFPEDLPLLKEDISDGKVVYKVVFKEQDMVKHVGEERPFDLQHYYQHPEEYKSRF
                     FDYLENMSVLVHGSYWDARYPRVVSKNEIARLYSKSNPKLVVVGDISCDPDGGVEFTH
                     KGTEISDPVFVYNPATGIPAMGFDGDGVCVMSVDILPSELPRDASRGFSDALRPFVES
                     IVECDFNNSYENLTIAPAIKRALILHNGELTKPFEYISKYF"
     gene            30227..30760
                     /locus_tag="PKY63_03625"
     CDS             30227..30760
                     /locus_tag="PKY63_03625"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HPE99736.1"
                     /translation="MPQEAPRRFVSVIGPSYCKVSDPIYAFARELGTKLVDHGFTVFS
                     GGRRGIMEAVFRGAHESGNYSFGATVGILPDSNRHSANRYTDIAVATGIGFARNSIVA
                     QSGDVIIALGGGSGTLSEIAFAWQYGKKIIAASNFDGWSRNLSGKKLDHRRRDTIIQA
                     KTVDEIIAQLNKLLVVK"
     gene            complement(30999..32327)
                     /locus_tag="PKY63_03630"
     CDS             complement(30999..32327)
                     /locus_tag="PKY63_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE99737.1"
                     /translation="MEIFRRSINQLIEWQNKPKRKPLILQGARQVGKTWLLNEFGKKQ
                     FKQVAYFNFEEQPELKQFFTGTRDAKRIIGNLSLVYGKSIEEETTLIIFDEIQECNEA
                     LNALKYFYEKVPGYCIVSAGSLLGVALTSGSSFPVGKVDFMTLYPISFKEFLSAASPD
                     LLPVVNNISKPEPIPEIFFNRLLEQFKRFFISGGMPEAVVSLLQNQDVNETQTSLQNI
                     LNAYALDFTKHASVNDFHKINYIWTSLPSQLSRENKKFLYQSVKPGARAREYENALEW
                     LKNAGLIYKINKVTAPRMPLSAYDDLSSFKVYMNDVGLLRRMSLLNPIAITEGNRMFT
                     EFKGALTETYILQSLIQQFEGVPRYWTSGNQAEIDFLIQYENEIIPVEVKSDENIRSK
                     SLAVYRKTYSPGISIRYSLRNLKLDDGVLNIPLFLADSTFDLIRTYYSPK"
     gene            complement(32523..33749)
                     /gene="rocD"
                     /locus_tag="PKY63_03635"
     CDS             complement(32523..33749)
                     /gene="rocD"
                     /locus_tag="PKY63_03635"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPE99738.1"
                     /translation="MAERLSSAQLMELEHHYGAHNYHPLPVVLERGEGVFVWDVEGKK
                     YFDFLSAYSAVNQGHCHPKIVGALIDQAKKLTLTSRAFFNDSLGVYEKYVTEYFGYDK
                     VLPMNTGAEAVETAIKLTRKWAYLTKGIKRYEAKIIVAKGNFHGRTTTIVSFSDDPSA
                     YDDYGPFTPGFIQIPYNDLAALEKELQQDPNIAGFIVEPIQGEAGVFVPDEGYLKKAY
                     DLCKKHNVLFVADEVQTGIARTGKMLCVDHEGIKPDMVILGKALGGGAFPVSAVLAND
                     NIMLNIKPGEHGSTFGGNPIAARVAMAALEVIKEEKLVENAERLGKIFRDEIRKIDSP
                     MIELVRGKGLLNAVIIKPMNGKEAWDVCLKMAENGLIAKPTHGHIIRFAPPLTITEDQ
                     IMEAVAIIKKSILSFH"
     gene            complement(34426..34851)
                     /locus_tag="PKY63_03640"
     CDS             complement(34426..34851)
                     /locus_tag="PKY63_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99739.1"
                     /translation="MNVQLFSFRALTAVFTILLLFPGCQDAKRKAIDLKMQGIDLNYK
                     RQFDKAIETLHKSLEYDDQDPETYFYIGTSYFGLNQLDSAFEYYSKAIETDSTFGQAY
                     VNRGRMYKERNDRNNACRDWLKAEECGIKSIKEETKFCK"
     gene            complement(34853..35956)
                     /locus_tag="PKY63_03645"
     CDS             complement(34853..35956)
                     /locus_tag="PKY63_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99740.1"
                     /translation="MKKIIFLSVLALTLLFAISCKDDDDTTDNEFELNEGQSNWSKRL
                     LVQRPYSNAPQALPGEYCEVSTGFLYVKGDTVSCYASLKLNTEYTYIFTSQNAGLNWT
                     MHSSQNGYILSAAQSGEKSYALRNYLSNVEFGSGQQYGGSWTWQNMAGNASKIRILSA
                     DTLYAFGADGIRVSQNGGLNWTINSTLNATDIQNYDENTLIGIFGNEIRVSDDLGSNW
                     TLLSTVTQTLYCLYKNPDGHWFAGGKNGCVLMSADNGNSWTQKFILTNIYPYAGLAQT
                     QAVHFLDANNGFAAIACPTVVNCGDSFDEMTGCILRTLDGGQTWTVNYRSEFIRYSRL
                     LSATGPNVMALGAQYRDNIVSGIYLTLTTTLGN"
     gene            complement(35953..37296)
                     /locus_tag="PKY63_03650"
     CDS             complement(35953..37296)
                     /locus_tag="PKY63_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPE99741.1"
                     /translation="MGAFHAYDIRGVYGKDFSSEDVYRMGFFLPSVLNAKRWLVGRDV
                     RESSPEIHRQLLKGLTDAGCEVSDAGLCTTPMVYWGTAKFGFDASVMITASHNPAEYN
                     GLKISAANAIPVGYDTGLNILEQKIKEIPAPSGTPGNVISFDFLKDYVAFMLPYKNDI
                     KNLKIVMDCSNGMSSIIVKDIFGSHPTYLFDEPDGRFPNHEPNPLDMKNIVQLQQKVQ
                     ETGADLGVIFDGDADRVMFTDELGTFIPPDLLIGLLGHYFLEEKGLKGKVIQDIRTSK
                     SVAEYLVPMGAEMKIWRVGRAYAANMLREIDGIYGGELAGHYYFKDFYYSDSGMLASI
                     LIINLFAQFKREGLTASAVVGRIRKYENSGEINFRIAEKQKAMDAVKNYFESEASPTL
                     FLDFDGYRVEFPDWWYNIRPSNTEPYLRLLVEAKTTTLLDEKVNKMREILSTFQS"
     gene            37637..>38337
                     /locus_tag="PKY63_03655"
     CDS             37637..>38337
                     /locus_tag="PKY63_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPE99742.1"
                     /translation="MLEAGIGCFNQKYLKAFKCVALVGPDRNAHTFRIILYFSRDLGK
                     KMYELSNHLGNVLTTVSDIKLTADGNTDGVVDSYAAVITSAQDYYTKLKLQLVQRRTA
                     ISYLDNAVETCCSALQFGLKYKSTRYYPFGSLEPGRTYSSDNYRFGFNGQEMDNEISG
                     QTGTHTTAMFWEYDSRLGRRWNVDPLSKLAAGWSPYRAFFCNPILYSDPFGLFETRKE
                     ARQYRRENGLRGKIN"
BASE COUNT        10667 a         7760 c         8685 g        11025 t
ORIGIN      
        1 agatttgttg aaagagcaag agtaacagta tccatggttg cattgagtgt tgcggagctg
       61 atgccggcaa gaccggtata gttggcaact gtttcagcag ttacatcatc catggcttcg
      121 ctgaatttca cttttacaac ggaatgtgtt tcagcccatg cagcaatggc tgtaggcgga
      181 acaatgtcgt tgactgaata tatcactctg aatgattggc ttgcagccat tacattgcct
      241 gcagtgtctg caactccggc aatatgaacg gtatcggcaa cattatcaat aagaggtgta
      301 ctcagagcaa gagtaacagt atccattgct acattaaggg ttgctgtacc aattccggca
      361 agtccggtgt aattggcaac agtttcagca gttacatcat caacgacttc gttgaataca
      421 accttgatgg tgttcaaatc tgtagcaaag gcagtatcaa cgactggtgg tgtaaggtcg
      481 tcgggaattt cgcatccctg tccgggattg gcaaatacag gtgttgtttc gatggtgcca
      541 tagggcaaat ggccaagact ccagtttgta acatcattgt tatcagtatt cggatcgcac
      601 aaagtgaggc tatagccgcc gccattggct tcggtcggcc atgaagctgc tgcatatcga
      661 acggtgtcaa ccaaaagtcc gcctggactc agaataacaa tcgctgctgc tgtattgctg
      721 attccggttg tattaccctg aataaatgtc tgtccgtaaa agatattaca ggaatttgcg
      781 tcgggtgaaa caagtacata atcaccggga ttcataatca acgatgagtt aaaagtgtag
      841 gcacttgtat tgtatttgag tgtgtatccg ttcaaatcta caggtacaat atcgttgttg
      901 tagatttcaa tatattcaag cgtatctaaa ctgggcggat tgtacataat ttctgtaatg
      961 accagggctt ttgttgttgt tacggtatca agcatcagaa caaacatttg ctgagcggtc
     1021 attgcatttc ctgcagtatc ttcgatattc tgaatataga gggtatcagc aacagcattg
     1081 atgagatttg ttgaaagagc aagagtaaca gtatccatgg ttgcattgag tgttgcggag
     1141 ctgatgccgg caagaccggt atagttggca actgtttcag cagttacatc atccatggct
     1201 tcgctgaatt ttactttaac aaccgcatgc gtttcgggcc atgcactgat ggctgtgggc
     1261 ggagttacat cgagaacacc aaactgaaca ggaaaacttt gagcagcggc cattgggttt
     1321 ccagctatat cttcaacatt gtttacaatg attgtatcgg gaattccgaa ggtgagcggt
     1381 gttgtaagta caatatgaac actgtcaaga ccggttgtgc gagtggcact ggttatggca
     1441 ccaacgccgg tgtagtttgc agtaacttca gctgaagctc caacaggttc ggaaaagcgc
     1501 acccagatat cgctgagtga tgtagtagtg gcagagatca ctgtcggggg aataatatca
     1561 gtggcagaag ttgatgtagc ggtgatgctg aaattatcaa gtctgttatt tccggatgat
     1621 gatgatgctc caacaaaaat cactttgata taaacattgg cagcttcgtc cagaatgttg
     1681 tagctgctta aatctattgt ttcagtaagc caagtggatg atgtatttgc agcagtagta
     1741 tcaaaacggg tgaatgttat tccatcggta ctgtattcaa gataatgttc attgaatccg
     1801 gttccggttc cccgggcagc ataagaaata attggatttt cataccccgt ggtagaaaac
     1861 tttaatacaa tgccttttcc attggctgca gaattaatta gagcaatcgc gcttgaggcc
     1921 aatggtgttg ttcggtcatc atttaatgta cttcctgaaa aggaattaat ttcagtattt
     1981 gatgcggcac aaatccatgc agatgaaccg tttgttccat cggcataaat tgcagcaatg
     2041 ttgtcctgcg cacctgaatc ggctggaatg accagctctg tgtttggcgc cgctttaagc
     2101 gtgtcgaaat gccatgaaca caataaatcc tgcgacctcg ctgccagcgc tgccatcaga
     2161 atcattgaaa tcagaaatag ttgtttcata attgaaagta tttagtttga aaaaatgtaa
     2221 cattgaacct gtcttgtttt tcaaagacaa ggactgcaaa ggtacaaaaa ttagtgtcat
     2281 gtttttacta ttaaaatgtt ataaaaacac tattaatatg aatgtctgat ttgttaatat
     2341 gaaaagtgta atagtttcga taattaaatt cagacattta tattaagtca gactcttata
     2401 aaatattgat tataaaatat tatatacctt tgaaacctga aacaaaattc tttacagaaa
     2461 tgaaacacga taaacagatt tttgacctga tagaacagga aaaagcccgt cagatgcacg
     2521 gaattgaact tattgcatcg gaaaattttg tcagcgaaca ggtcttgcag gccatgggtt
     2581 cagtattgac aaacaaatac gccgaaggtt atcctggcaa gcgctattat ggcggatgtc
     2641 agattgttga ccagacggaa caattagcca tcgaccgtgc atgcaaactt ttcggagctg
     2701 aattcgccaa cgtacagccc cacagcggtg cgcaggccaa tgctgctgtg ttttttgcct
     2761 gcatgaaacc cggtgatacc tttatggggc tcgatctcgc tcatggtggg catctttcgc
     2821 acggctctcc tgtaaacctt tcgggcatca attacaaacc ggttgcctat catgtgaaag
     2881 aagaaaccgg cttggtggat tatgacgaaa tggaaaaact tgcaaaagaa tgcaagccga
     2941 aaatgattgt tgctggtgct tcggcctata gccgcgactg ggattataaa cgcatgcgcg
     3001 aaattgctga ttctgttggc gcgcttctca tggccgacat tgcccatcct gccggattga
     3061 ttgctgctgg tttgctcaac aatcctgttg aatattgtca catcattaca acaacaactc
     3121 ataaaacatt gcgtggacct cgtggtggaa tgattctgat tgggaaagat tttgacaatc
     3181 catggggcat taccactccc aaaggtgttg tgaagaaaat gtccgaagtc attaacagcg
     3241 ccgtatttcc cggacaacag ggcggcccgc tggagcatgt gattgctgct aaagcagttg
     3301 cttttggcga agctctggaa cccgaatata aggaatatat gcagcaggtg agaaaaaacg
     3361 cagctgcatt ggccgatgcc tttattaaaa aaggatatca catcatcagc aatggtacag
     3421 acaatcacct gatgctgatt gatctccggc agaaatttcc tgaacttacc ggcaaaaaag
     3481 ctgaaaatac gctcgtgctg gctgatatta ccattaataa aaatatggtt cccttcgatt
     3541 cacgcagtcc tttccagaca tcaggaatcc gggtcggtac gccggctatc acaacccgtg
     3601 gcctgaaaga aaatcacatg gctcagattg ttgatctgat tgataaagtg ctctctgatc
     3661 ctgataatga agctgtcatt gccgatgtca gaaagcaggt gaatgccatg atgaaggatt
     3721 ttccgttgta cgcgtggtaa tatttaattg attttatcag aattatgagc agtaagacat
     3781 tgttgattat gcgccatggc aaagccgatg gtcaggttgg tagtagaaac gatattgagc
     3841 gtcccctcac tgagctgggg cgtaaacgta ccgatttgat tgcccgcgaa ctgataaaaa
     3901 agaaggtgtt gccgcaattg atcatttcaa gtcctgctct ccggactttg cagacatcgc
     3961 agcaaatagc tgccggactt aatctcagtg cggatgctgt tcagataaac gaagatctgt
     4021 attttaaaac agtcaacgat tattttgatg cactctatgc tgcccccgat gaaaaaaact
     4081 gcattatgat tgttgggcac aatccgctgg taacgcaatt tgccaatttt tttctttctc
     4141 agaaagtggt tgatatgcca accagcggat tgattgcatt gcagagcgat gctcgtacct
     4201 ggcctgagtt tgttattgct tttaaaaagg ccttgtttta tctcattcca aaaaatcttg
     4261 agaatgaaaa aacgtgaaaa tgatttggct ggtctttatg taaaccgcga attaagctgg
     4321 cttgatttta atgaccgcgt tttacaggaa gctgaatgcg ccgacagtcc gctgcttgaa
     4381 cgaataaaat ttctcggaat tttttcaaac aatcaggatg agttttttcg tgtccgcgtt
     4441 gctacactga aacgtattgt caagtttcga cgtaagtcgg aatatgaatt gtacagatat
     4501 ttcagtcctg aaaaagtttt gacagaaatc aatcagcgta ttgttggtca gcaaaagcgg
     4561 ctcgctcagg tttacaatca tctcgaaaag gagctcgccc gcgaaggggt acatattatc
     4621 aatgaaaaag aagtgaagta tgctgaacat aaatgctttg taagaaagta ttttgatact
     4681 gttttgcgta cctatatttt tccgattatc atgcgcaatt ataatgcgga taccatgctg
     4741 cgcgacaagt caatttatct tgctgtttca atgcgcaatt ctgcccgcaa tacaaaacct
     4801 gagtatgccc tgctcgaagt cccttcagaa caattgtcgc gctttgttgt tttacctgag
     4861 atgaatggtg aacaatatgt gatgtatatc gatgatatta ttcgctattg tatgcctgat
     4921 attttcaaga gttttgatta cgatgaattc aaagcttata ccatcaagtt tacacgagat
     4981 tcggaacttg aaatggacag cgatatatcg aaaagttttc tcgaaattat ttcggatagt
     5041 ctcaagcaac gtaagaccgg caatccggtc cgctttgttg ttgatcgtga tatgcccgaa
     5101 gaaatgctgg agtatttttt tgacaagatt ggtcttaccc gcgatgatac acttgtttca
     5161 agcggacgat atcacaattt taaagacctg atgaattttc catcgctcgg gaaaaatcat
     5221 ttgagttttg aagatcgaga accgatattg catacgcgtt tagagaaggt ccgaagtgtg
     5281 tttgatgaaa tgaaaaagaa agatattctg ctttcatttc cacatcatcc gtttaaatat
     5341 gtgattgatt ttctccgcga agcatcgatt gatccggctg tgcgtagtat caagatgact
     5401 atttaccgga tcgggaaaaa ttccaacgta atcaacgctt taatcaatgc cgcgcgcaat
     5461 gggaaaagtg ttaccgtgtt catggagttg caggcgcgtt ttgacgaaga aaccaatatt
     5521 ctgtggagta ccaagctgca ggaggaaggc gtaagggtta tccacagcat tccggggctg
     5581 aaagtacaca caaaactcat tcttgtcaaa cgcgttgaag gcaaaaaatc ggtctattac
     5641 accaatgttg gaactggtaa tttcaatgag ataacagcat cgttgtattg cgatgtcagc
     5701 attatgactg ctgatcagac aatcgggctt gaaacggcac aggtgttcga aatgttcgaa
     5761 gccagttatc gttctttttc atttaagcag cttgttgttt cgccaatgga aatgcgtccc
     5821 tttattgaag gcatgattga taaactcacg gaatctgctt tgcgtggcga agatgcgtat
     5881 gccgccatca aactcaataa tattgttgac aaagatattg ctgataaaat atatgccgca
     5941 gcgcaggccg gagtgaagtt tgatattata tgccgcagcg cctgtgtttt gcaacccatc
     6001 aatgaaaatc tgcgcataat cagtattgtt ggtcgtaatc ttgaacacag ccgtattttg
     6061 tattttcgtt gcggacaatg ggagcgtgtt tatattactt cagccgactg gatgaaaagg
     6121 aacatggacc gacgtattga agtgatgtgc cctgtgttgg ataaggatgc cgctgaactt
     6181 ctgaaaaaaa tattgtttgt tcatttgcag gataatgcaa aagcccgttt tgttaatggg
     6241 aaaaagaaca atatatatat cagaaacaat gggcctttat ttaattcaca ggacgaagtt
     6301 tatcgtttga tcagatcggg cgattttgaa atatgagata tgcagctata gatattggtt
     6361 caaatgcggt taaatgcatg gtgggtactg ttatagacag cggatacccg gttgttataa
     6421 aagaaatgta tgcgcgtgtt ccggttcggc ttggcactga tgtgtttcag tcgggcgctg
     6481 tctccgctga atctgaaaaa aaactgctgg attcgctgag ggcttttgta ctgcttatga
     6541 aatcatggaa tataagtgga gctgttattg cggctacaag cgctatgcgt gatgcttcaa
     6601 atggtcatga cattctgaaa aatattgaaa atgaaaccgg cttgagaaca tggattatca
     6661 aaggtaaaat tgaagcagct ttgataacgg atctttttgc cggtttgggc gaaaagaaaa
     6721 agcagaaaat gatgattgat ttgggtggag gaagtactga agtaactttt gcaaaccctg
     6781 ataacaagag gaaatccaaa agttttcaga taggcgctgt gcgttcgctg atgcatgaag
     6841 ccagcagagt tgaattgaaa aaaatgcttg attttattga aaataatatc gatccgtttg
     6901 gtcatcttat tattgcttcg ggtggaaata tcaatgcctt gaaatcgcat ttcgggctgg
     6961 cagagcccat gtatctgtcg tttcaggaat tgcagcgggc gtatgatcaa ttgcatccgc
     7021 ttactgtaag cgagcgtgtg gagcgctttc tcatacatcc cgaccgcgcc gatgtgattg
     7081 ttcccgctgc agaagttttt ctgaaaatca tgaaatttgc caatgcgcaa aagctcatgg
     7141 tgcccaaagc cggtctggcc gatgcactca tattaatgca gcatcaggga aaaacaccgg
     7201 agcaatacta gcacaacgcc ttgcgagtta aaagtttata atgttcgtaa agttgaaagg
     7261 caccgtaaaa acatatggca tgatctgaat gcgctctcgc tctgattccc gctctgtatt
     7321 tgtttggcaa agcttcgctc tcgctctgat tccctctctg tatattgcat gcggatcttc
     7381 gctcccacac tgtccgccgc ggcgaaggtg ttttatttta tggtatcaac gtttactcca
     7441 aaaattagga tagttggttc ctttgaaagg gactacaggc gtagtccaac aataatagcc
     7501 cgaagaattc acgttttacg tgatgccctt tagggctgcg gggaaaaagg agacgcgaag
     7561 cacctttgag atgtatcgac acgcggcttg ccgcgcaaaa agcgaagcaa ataaatcaaa
     7621 attacgctgt catcaagagc cccgccgctg tcacattgag ccctgcctgc tgtcacactg
     7681 agcggagtcg aagtgttaac agcaggataa actccgtcga aatgtcgaca gcggcgcgca
     7741 gccgcagccg ctttagcgtc aggcagggca aaatattcag agttgttatt tttattctgg
     7801 aaatttttca tagttttgga aagcaataaa gttgcatttg tacattgaat tcttccttgc
     7861 tgaagcaaaa taaataagaa aaagaaatag ttatgaataa tgataaatca ttggttgttt
     7921 ttgataacaa gaaagtccgg cgtttctatg atgaagaaac cgaagtttgg tatttttcag
     7981 ttgttgacat tgttgaagct ttgactgatt ctgtgaatcc gactgattat ctgaagaagt
     8041 taagaaagcg tgaccctgaa cttggaacct acctggggac aaattgtccc cagatagaaa
     8101 tgctcacaaa tggcaaaaag cgcaaaactc tagctggcaa tgtaaaagac atttttcgaa
     8161 ttatccaatc aattccttct cccaaagcgg aaccttttaa acaatggctg gcaaaagttg
     8221 gctacgaaag agttcaggaa atgaatgacc catcgttaag catggacaga gccagggaaa
     8281 actggcagaa aatgggccgc agtgaaaagt ggattcaaca acgaatgaca gggcaggaaa
     8341 cccgcaataa gctcactgat tattggaaaa acgcaggtgt aaaagaacaa gatgaatttg
     8401 ccctgctcac caatattatt cataaagaat ggacagactt aactgtaaaa cagcataagc
     8461 aattgaaagg attgaagtct cagaatttgc gggatcacat gagcgaagct gaattgattt
     8521 ttacagctct ggctgaatta tcgacccgac aaattgccga cagtgaacaa gcaaagggat
     8581 taatcgaaaa tgcaaaagcc agcaaaaagg gtggagcagt ggcaaaaaat gcccgaaaag
     8641 aattagaaac taaaactggc aaatcggtca ttactggtga aaactttctg cctccggcaa
     8701 aagagaaaaa gaaactaccc ccaaaaaaga aataagtacg ttttggtaat ttgacaacaa
     8761 agaatactat tgaacaatga gttgcacgca gaactaactt cctgatgccg agtttgatgt
     8821 atatgctgta tttaaagcgt tgaatttttc aattatcttt gtaagatgaa ttctccgcgc
     8881 gatttatttt tccgtcatgt ggcacaaaca tcgcctgaac cgctggcact cgaaattgtg
     8941 tcggcgaatg gaagcaaatt gtttgctgcc gatggaatgg aatatcttga tttcatttca
     9001 ggaatttcag taagcaatct cggtcacaat catccggaaa taaatgcagc ggtgattgag
     9061 caggtgaaca agcacacaca tctgctggtg tatggtgaat ttattcagag tgctcaaacc
     9121 ggccttgcag agctgctttg cagtgtgtta ccttcatcgc tgaacaatgt ttattttgtc
     9181 aacagtggtg ccgaagccat cgacggcgcc atgaaactgg ccaagcgcag caccggacgt
     9241 ttcgagatac tgtcctgtcg caatgcctat catggaagtt ctaccggccc actcagcatt
     9301 atgggaagcg aagaatacaa aaccgctttt cgcccgttgt tgccgggtac cggtttgatt
     9361 gatttcaaca atcccgatga tttaaaatgt atttctgata aaacggctgc ggttgttatt
     9421 gagccatttc aggccgaagc cggtgtgcag atacctgata aagattattt tgctgctttg
     9481 cgcaaacgtt gcgacgaaac aggcactttg ctgattctcg acgaagtaca gaccggattt
     9541 ggtcgtaccg gaaaaatgtt tgctatggag cattttggca ttgtaccgga tattctggtt
     9601 cttgcaaaag cgttgggtgg cggatttccg ctcggnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnattat cagtgaagat gaaatccgtt acggatgcga aaaaatgatt
     9781 aatattgtac actcaattca ataattcata tgattaataa tccggataga aaatttttgc
     9841 agatggccgt agatctcgct gttgaaaatg tccgcagcgg caaggggggg ccttttggtg
     9901 ctgtgattgt gaaggatggt gaagtgttgg caactggtgt aaatctggtt acaagcaata
     9961 acgatccgac ggcacatgcc gaaattactg caatccgcaa tgccacggct aaactgaagg
    10021 attttcaact gaatggttgc acgctttaca gcagttgcga accttgcccc atgtgtttgg
    10081 gtgccattta ttgggcacgt ccggaacggc ttgtttatgc tacctcgaag cacgatgctg
    10141 ctgatgcagg atttgacgat gcttttattt acgatgaact tgaattaccg attgctcaga
    10201 gacatctgaa aactgagtat tgtcccgagg aaccatataa attacctttt caaatctgga
    10261 aagaatctgc agcaaagaca gagtattgag tctgatgtca atttctttaa atgctttttt
    10321 attgaacggg cattcttttg ttttgtaaaa gcaggaatct gtttacaact tgacaattga
    10381 taagcaggat cggagtgatg gtgaattcag gtctgtgagg gaatcgcata ttttagtgta
    10441 aattgtcatg aatcaacaaa ttaacatttc atgtaataaa tttgtgctta caacgtaaaa
    10501 tttttttgga aaaacagaaa tattatttag ctttgcgttt acattgggta atatttttta
    10561 tatggaagaa tttgacagaa aagaacggga tgagatttac tcgagacctg tacgtgccgg
    10621 aaagcgcaca tattttttcg atgtaaagga aatgagaagt ggcgaaatgt acatcaccat
    10681 tacagaaagc aaaaaacgtt ttaacgagga aaatggcaaa tttttctacg aaaagcataa
    10741 aatatttctg tataaggaag attttgacaa gtttgccaaa gggcttgatg ctgttgtgaa
    10801 ttttatccgt actggtgaga aagcgccgga ggaagatttt catgaatttg ccggccagaa
    10861 tgagtttggt agtgagatga atcaggaaca atctgatttg tagttctgtt cacatattta
    10921 gttattccgc tcataaaaaa gtgcggaata atttgtattt ttgaccgttc aatttaaatc
    10981 gcgcaatttt ttatgggaaa agtcatttca atcgccaatc agaaaggcgg tgtagggaaa
    11041 accaccagtg ctatcaacgt cagtgctggt ctggctgtgc tggagaataa agttttgctc
    11101 atcgatgctg acccgcaggc caatgccact tcgggacttg gcttcgatcc aaagaatatc
    11161 agaaccagta tttacgaatg tattgttgac agtgtgaatc cggctgatgc tattttacac
    11221 accaaaacac cgaatcttga tattctgccg gcccacattg atcttgttgg tgctgaaatt
    11281 gaaatgatca acatggaaaa ccgcgaacgt atgatgcgaa aggttgtgcg gcaattgaaa
    11341 gaccagtatg atttcattat cattgactgt tctccatcgc tcggtctgat taccgttaat
    11401 tcgctcactg cttcagattc tgtgctgatt ccagttcaat gcgaatattt cgctctcgaa
    11461 ggccttggaa aacttctcaa taccataaaa attgttcaga cacgtttgaa tcaggatctg
    11521 gctatcgaag ggattctgct taccatgtac gatgtgcgtt tgcgcctgtc caatcaggta
    11581 gtcgatgaag ttcgcagcca ttttaaagaa atggtttttg agactatcat accgcgcaac
    11641 acccgattgg gcgaagctcc ttcttttggt gaaagcatta ttatgttcga tgcagaaagc
    11701 aagggatcta tcagttatct gaatcttgcg cgtgaaattc ttcagcgaaa caacatgata
    11761 aaacagcagg cataacaact ttattaaaac gattgaattt aaaaattgat gaatacacga
    11821 aaaaatgcat tgggacgcgg actgggagct cttcttgagg gcgcaaatga tgaatcggca
    11881 cagggtgttt ccgcgcctgt aattgctttt ccgatgattg atatcgacga aatagaagca
    11941 aacccgtatc agccacgcga ccgatttgaa caggaagcat tgaagggatt gtccgaatcc
    12001 atcaaacatc aggggctaat acagcccatc actgtgcgta aacttaaaaa cggaaaatat
    12061 cagcttattt ccggtgaaag acgtttgcgt gcttcaaaaa tggctggtct tacgcaaatc
    12121 cctgcattta tacgtgaagc cgacgatgaa gctgttctgc agatggctct ggttgaaaat
    12181 attcagcgcg aaaatctaaa cgccattgag gttgcgctta ctttcgacaa gttgatggat
    12241 gaatataaaa tgacacagga aatgctcagt gagaaagtgg gtaagaagcg aaccactgtt
    12301 gccaattacc tgcgcttgct taaattaccg attgaaattc aggccggaat ccgtgataat
    12361 aaaattacca tggggcacgc ccgttctctt gtaggacttg aagatgaagc aaaaatgaga
    12421 attgttttcg atcgaattat tgctgaagat ctttctgttc gtcaggtcga agacattgtg
    12481 cgcgggatgg ttgctgtacc tgaaaacaaa acggcaaaga aaaaggaaaa agatctggcg
    12541 catgaggaat tgcagaaatc catcgctgct aaaatcggcc ttaaggttca ggtaaaatca
    12601 ggacttaaag gaggcaccct gaccatccat tttaaaaatg aggaagacct gaaaaaaatt
    12661 gaaaccattt ttcattgaca atgaataggt ttgctgcgac aatactactg attttcgcat
    12721 tcctcaatat tgtcaaagcc cagaatattg atgacaccac ttctgtggcg cattctccca
    12781 gaaaggcaac catcatgtcg gcttgtttac caggacttgg acaggcatac aaccacaaat
    12841 actggaagat cccgattctt tatgcaggtc ttggaggcgc tggatacgcc attgtgtgga
    12901 acaataataa atatcaggat tttcgtaagg cctatattgc tcgcaccgat acgattgatt
    12961 ccactacgga taattttcct cgctattcgg cttcaaatct tcttgacctg agaaattact
    13021 atcggcataa tctcgagttg tcggttatca ttatcactgc ggtttatatt ctcaatattg
    13081 tggatgcttc tgttgatgcg catttatacg atttcgacat cagtgatgac ctgagtctgc
    13141 gcattcagcc atcggtctgg aacgttgggc cgggtacttc ttctttcggc ggcggacttt
    13201 cattaacctt caaactcaag ccatgaaaat attcataatc ggttatggta aaatgggtaa
    13261 aatgattgaa tctctggctg caaaagaggg acatgaaatt gttgccgtgg tcgatgcagg
    13321 gcagttctgg cctcgtttta ccgatcacga tcgtcctgac gttgccatcg aattcactca
    13381 acccgatgag gtaagcgata atctgaagca ttgtattgaa tggggaattc ctgttgttac
    13441 cggcaccaca ggttgggaag actctcgtga agaaatcaga aatttatgtc tgcagaaaaa
    13501 tgcagctgtg atgtttggca gtaattacag cattggagtg aatgtgtggt tcaatgcttt
    13561 gaaagcagtt gccgcaggcc ttgccactac atcaggatat caggtttcca ttgatgaaac
    13621 acatcatgta aacaagaaag ataaacccag tggaacagcc atcaccgctg caaatctcat
    13681 ttatgagcgc atgcagcaat atgatggatg gagtttgaaa gatgaaaaca ataaaattca
    13741 tatcgaagcg catcgcagac aggatgtaac cggtatgcat tctgtccttt tttcatcgga
    13801 gatggatgag gtggaactgg tgcatcgggc tcacagccgc gaagggtttg caaaaggagc
    13861 aattcgtgct gcagagtggc tttgcaacaa aaagggtttt tttgatttct caacagtctt
    13921 cgaagaaatt ttcgccttcg gcaataaata gtggattaaa cgttattcgt aaaaactgaa
    13981 aaatatggac ttggttatta ttttcacatt ggtatttttt ctgctgactc ttgtcagtct
    14041 tgcatttatt ttccgcaaag ctgggcagtc tccttttctg gcgtttgtgc ctgtgtataa
    14101 catcatcatt tggttgcgta ttgtgaaaaa accactctgg tggctcattt tcactatcat
    14161 gccatacatc aatgttttta tggtcatgct cctgattgta gaaacgctta aggcattcaa
    14221 caaacacagc atcggagcgc aggtattgtc cattatgttt ccgtttttct atctgcctta
    14281 tctggcattt aataaaaatc tgacgtatat ccatcccgat cagcaggaga aaagaaaaaa
    14341 aacattttcg cgcgaatggg ttgaggccat tgtgtttgct gttatcgcag ctactgttat
    14401 ccgcatgttt tttatcgaag cttataccat tccgacttcc tctatggaaa agtcgcttct
    14461 ggtgggcgat ttccttttcg ttgacaagat gacctatggc tcacgaacac ccatgacacc
    14521 tttttcgttt ccttttgtgc atcatacttt gccgctgacg aaagaatcaa aaagttatat
    14581 cagctggatt cagcttcctt attatcgtta tccggcattt agcaaaatag agaataatga
    14641 tgtcgtcgta tttaattttc cggccggtga cacggtgatg ctgaaaaatc aggccaaaac
    14701 gtattatgga atggtgattg aacaggccgt aggaatggct caggaagccg gaaaagacct
    14761 gaaagattat cctatataca tgaacctggc tcgcagcgcg gttcttcagt ggtccgacat
    14821 tactgtgcgt ccggtcgata aacgtgagaa ttatatcaaa cgctgtgtgg ccattccggg
    14881 agatgttatt gaaatcaagg aaagacagtt gtttattaat ggcaaggagg ttgaaaatcc
    14941 tgaacaaatg gagtataatt ataagtttac acttaaacct ggttctatta tttcgcgtaa
    15001 aaaactgatg aatctgggac taagtctgga ggatattgaa aattcgaaac agactgctgc
    15061 atattatggt tattcttctg acgtgatgat tctgccaatg acttttgata tgttgcgtga
    15121 ttttaataat cagtttggaa cattgctcga tacggttcct gaagtaatga atgaaacagc
    15181 ttggcatcct tctatttttc cgcatagccc tgcttttccg tggaaccgtg ataatttcgg
    15241 accacttgag gttccgatga aaggacagac agtgaaaatt gatactacaa attatgtcct
    15301 ttattcacgc attattagta tttacgaagg caacacactt gaagtgaaaa acggcaaagt
    15361 gttcatcaac ggaactgtag ctgatactta tactttcaag atggattatt attttatgat
    15421 gggagacaat cgtcataatt cggccgatag ccgctattgg ggctttgttc cggaagacca
    15481 tattgttgga aaagctgctt ttgtctggtt gagtctcgat aaagaccgct cggtatctaa
    15541 tggaaaaatc cgttggagaa agatgctgcg acttgtgcac tgatgtgctt cgcacattag
    15601 tagtggaaag ttaataaagt ggaatgttta taaaggtgac gtaaacgctt acggcatgtg
    15661 gcgcaacgcg caacgcacaa agcggggcgc atgtgtgaat atgagatacg atgcttttct
    15721 gtgcatgaaa taacaatact gcagcgttga tgagattatg cgattatgcg aatttgattt
    15781 gtcgcatcat cgccgtatcg catcatcgcc aaaatggacc ccgtctcagg gctgaaagct
    15841 gaccgctgat tgctaaattg tttgtcattc tcattaattc agtgtaattt tgcaaacttg
    15901 caatatggtt ccctctttta tcaataatct gaatgatgta caacgcgatg ctgcaacctg
    15961 caccgaaggc cccgtaatgg tattggccgg tgcaggatcc ggtaaaaccc gtgtgcttac
    16021 ctatcgcgtg gcttatctga ttcagagcgg agtacagcca tataacattt tggcgctgac
    16081 ttttaccaat aaggcagcac gcgaaatgaa ggaacgcata tttcaattgg ttggagatgc
    16141 aaaagctaac gcattatgga tgggaacttt tcattccatt tttgcgcgta ttctgaaaat
    16201 ggaaagtgaa aaaatcggtt atccaaaaga ttttaccatt tatgataccg atgattctaa
    16261 aagtgttgtg aaagcgattg ttcgtgaatt ccaaatggat gataaggcct acaatcccgg
    16321 ttatgtgctc aatcggattt ctgcagccaa aatgaatttg atcaatcctt cgcaatacaa
    16381 ggatgatccc gaaattcagt atcaggataa aatggcaaaa aaacctctca ttactgaaat
    16441 ttacgctgag tattggaatc gttgtaaacg ttcctctgca atggatttcg acgacttgct
    16501 tttcaataca aatttgttat tgcatcatta tcccgaagtg ttgaaaaaat atcaggatag
    16561 gtttgaatat attctggttg atgagtatca ggatacaaac tatgctcaat accttattct
    16621 gaaacgtctg gcagcactgc gcgaaaacat ctgtgtggta ggtgatgatg cgcagagcat
    16681 ttatggtttt cgtggtgcaa acattcagaa tatcctgaat ttcaataagg attatccctc
    16741 gttgaaaaca tttaaacttg aacaaaatta tcgttcaacc aaaaccattg ttgctgcggc
    16801 caattctgta attcagaaca atcaatcgca gcttcataaa atggtgtgga ccggaaacga
    16861 tgaaggtgag ccgatttcgc ttatcaaggc ctcaaatgat attgatgaag cgttgttaat
    16921 tgccaattcc atctttcaca cacggatgac ctatcatttg aaaaatagtg attttgccgt
    16981 gctttaccgc accaatgctc agtcgcgttc gatggaagat gcattgcgca aactcaatat
    17041 tccttatcga gtgtatggcg gtatgtcgtt ttacaagcga aaagaagtta aagatctgat
    17101 tgcgtatttc cgcattgtca ttaatccaaa cgacaatgaa gcattctatc gcattgtgaa
    17161 ttatccggcg cgtggaatcg ggaatacaac attgcataag atatcggtgc atgccagcga
    17221 acaagaagtc cctgtctggg ctattctgca gaatatcgat atttatcatc atgatttcaa
    17281 tgcttcaacc atcaagcgtc tgaaggattt catggaattg atcagacatt atcaacagca
    17341 attgctcact tccactgcat gggaactggc gacaaagatt gctacgggca gcggattagt
    17401 gaaggaatta ttcgaggatc gttcgccaga aggcattgct cgatacgaaa atattgaatc
    17461 cgttttgaat ggtatccgta gtttcgagga tgaatatgaa caggccaatg atggtgaaaa
    17521 gccgtcgctg agcgactttt tacaaatggt ttccttattg acaaccgttg atgaagctga
    17581 tgataaggag aataccgacc gggtttcgtt gatgacagtg catgcagcca aggggcttga
    17641 atttccatat gtgtttgttg caggtctcga ggaaaatctg tttcccagct ccatgtcaat
    17701 gagttccaag gccgaaatcg aagaagaacg acgtttgttt tatgtggctg taacacgcgc
    17761 aatgcaaaag cttacattga gttatgccga tacacgtttg aaatggggtg agttgaattt
    17821 ctgcactcca agccgttttc tcgaagaact cgattcctcg ttacttgata ttcctcaaaa
    17881 attttctccg ggactaaagc aaaaagatga ttcgtcgttt cgtatgaaat tgcaggatgc
    17941 atcttcggaa aacaacttca aatctaagta tcagaaaggt gcaggatatt cgttcaataa
    18001 aaaagtgtcg gataagagtg gaccggaatt taaaaagaaa gaagtaccgc agcccaaatc
    18061 agcaccagca ttcaatgata gcgaactggc cgatgtagat gatatttccg aaggtatgcg
    18121 tgttgagcat gcgcgatttg gtgtcggcac ggttcttcag attgaaggaa acggtcagaa
    18181 caggaaagct ttggttgaat tcgacttatc gggccaaaaa cagcttgtac taaggtttgc
    18241 aagacttaag actattcaga attaaaatca tccggcagtt tttcttgatt gtcggaataa
    18301 aatcagatat ttgtaaaaca aagccatttt ccatggaata caacacacag cgcgaacatt
    18361 tagaaatgac cgaatacggc aggtatgtta aacaaatggt tgagtttatc aaaaccatgc
    18421 cgggcaaaga atcgcgaaca aaggccgcca atgccattgt tcatgcaatg atgactgttc
    18481 agcccggccc gcgggacatg attgattata aacgtaaatt atgggatcat ctgatgctga
    18541 tgagtgattt taaactcgat gttgattcgc cttatcctct ccctgagcca aacaagaaag
    18601 agattgcgca gtttctgaat tattctgtgc cttatgaagt tcgtttccgt ttttatggtc
    18661 gttatgttga aaaaatggtc aaaaaagctg ttgaaatgga agagggggaa gataagaccg
    18721 agttggtgcg gttgattgca aacacgatga aaaaattata tctcacctgg aacaaagaca
    18781 gtgttaaaga tgaaatcatt attgaacaac tcaatatttt atctgaagga agattggcat
    18841 tggatccggg ttatgtgctg caggatacta ctgaatttgt gaaaaacaac aaaacgcaac
    18901 agaaaatctg gactcagcaa agcaagaaaa acaagaaaaa taaataccgg aaattaaaac
    18961 aataattatg agcagctttg aaattactgg tggaaaccgg ctttcgggca ctattacgcc
    19021 tcagggtgca aaaaacgaag cattgcaggt tatctgtgct gttttactga cagatgaaaa
    19081 gatatgtatc tcaaatgttc ctgacatccg tgatgtgaac aagcttattg atgttatgcg
    19141 ctttcttggc gtcgatgtga gtaatccttc cactggaacc tgggaattta aagcagacaa
    19201 tgttcaaatt gattatcttc aaactgatga gtttcgtcag aatgccggcg tattgcgcgg
    19261 ttcagcaatg atcctcggcc cgatgctttc gcgtttcgga gtgggggcca tccctcgtcc
    19321 cggaggcgat aaaattggcc gtcgtcgtat ggatactcac tttattggtc ttgaaaaatt
    19381 aggcgcaact ttcgaggacg gtgatccgac attttatatt gcacgtcgca aaggcagact
    19441 gaagggggct tttatgctcc tcgacgaagc ttctgtaact ggtacagcca atattgttat
    19501 ggctgctgtt cttgcagaag gaactacaac tatttacaac gccgcctgcg agccgtatct
    19561 gcaacagttg tgcaatatgc ttgtcagaat gggagccaat atcgaaggca tcggctccaa
    19621 tttattaacc attcatggtg ttgaaaaact caacggaact gaacatactt tgctccctga
    19681 tatgatcgaa gttggctcct tcattggtct ggcggcaatg actcaaagtg atattataat
    19741 taaaaatgtt tcagtaaaag atcttgggtt gattcctgat gtttttcgga gacttggaat
    19801 tacagtcgaa cagcagggtg ataatctgca tgtgccttcc attgatgtgt atgaagtaga
    19861 aactttccgc gatggctcta tcatgaccat tgccgatgct ccctggccgg gttttactcc
    19921 tgacttgtta tcggtagtgt tggtagtggc aacacaggcc aaaggtagtg tccttattca
    19981 tcagaaaatg ttcgagagtc ggttgttctt tgtcgataaa ctgattgata tgggggctca
    20041 gattattctt tgcgacccgc acagggcaac aatcatcggt cttgaacgaa aaacacagct
    20101 gcgcggaatt gaaatgacat cgcccgatat ccgcgctggt gcttcgttgc tcattgcagc
    20161 attatcggca aaaggaaaaa gtattatcca caatattgaa cagattgacc gtggctatca
    20221 aaatcttgat ggccgcttaa gggctttggg ggcggagatc cgacgcgtgt agaatttcct
    20281 cagggtacaa ttgagccgca cgactgcgtg gtgttccggt ttgagatgaa ataatcagct
    20341 ctgtgcgaag tttccaaatc cctacgctgt gcgaagtgtc aacttcgcac atgccgtata
    20401 aacgtccctt catccagctc ttcaaaagcc aggtttaatt cttcctgcgt attcatcaca
    20461 atgggacctc cccatgcaac cggctcgtga agcggttctg cagcaatcag taaaaaccgc
    20521 gcaccatccg gaccagcttt tacaacaaca tcggaatatg tatttctttg ttctgatgcg
    20581 gctttcatga gcatagcaca ggccttatct tcaaatttat gaagcgtttc atctgctgca
    20641 agagtcccgt caatcaaata caaaaacaac gtttcattgt cgggcgtttc attgtagatc
    20701 caatgtgatc cagcattcag agctacatcg atatactgca cttttacata atgacttttc
    20761 agtgcaccgg ccatatcttt gtacatgccc gaaagcacgc gcacggtcgc atgttcctct
    20821 ttgatgagtg gcacatcttt ttgctgaata tcgttgtaag ccggtgcgca cattttatct
    20881 ttctgcggaa ggtttaccca gagctggcat ccaagcatcc ggtccgacgc ctgtagcatt
    20941 tcctgatgaa tgattcctga accggcagtc atccactgac attccagatt gcggattacg
    21001 cctttgtttc cgagactatc gccatgttca acttcaccct gaattaaata cgtaacggtt
    21061 tcaatgccgc gatgcggatg ccatggaaaa cctttgatat aatcctgcgg attggtggaa
    21121 tcaaatccat cgagcatcag aaacggatcg aagtctgtga ttgtgcgcag ccccaatacc
    21181 ctgcgcaaat gcacgcccgc gccgtcaact gcctgttgtc cgcgtacggt ttgtgtgttg
    21241 attcgttttg ccatgggtca caagtttatg gcaagatacg aaaattttcg gaaatttcat
    21301 ggttacaata cacgcataaa tagggttgaa tgaagcacga attgtaagca tgaataattt
    21361 aagcaagaac cacactcagt ttataacggc tgttatattc tcagtcaaac gcaacaaatc
    21421 cggtaatatt ccatttgccg tctttttttt caagaaacaa tgttcgtccg ccaagagcat
    21481 atatatcgct ggttttaatc caacctttta cagcaccatc aagggtattt atataatgca
    21541 ttttgaaatc aaagccagca tcgggttgtg cattcattgt tgcttcataa tcgataagca
    21601 gaatatcgta cgaaagtgtc gcaacttgtt tcgatgactc atcgcagctt tcataaacag
    21661 gcgcattttt acggatgcat acaccggtaa aatacggatc aaaatacata tccggaaaat
    21721 gtgcggcagc tgattgatac aaacgaccat cagaggcata agggtattgg attgctgctt
    21781 caatcgtatc ttcatcaaaa atgcctttac agaaaactcc tccaggattg attgcatgca
    21841 gcattaagtc ccacacttca gattctttcg cattttccag atgccactgc gacataaaat
    21901 cctgtttgcc aaacataatt ccaccgaaac tggtcaccat tttcggatgc atgattgcaa
    21961 ataagcgggc agtgtccttt tcagtcagga tatttttcaa ctgtgctaca aatgtcacca
    22021 gcgatgtatc cttccctgtt tcatcaaccg gaaaaatttt atactcagct atctgtgttt
    22081 gtcccgtact gcttaaaaca cagatcagga gcaggaaaac ggaaacaatg gatttcatgc
    22141 ctgacgtttt aaagaatatt tcttttcaaa cttacgaaaa attaagcata ttcattttcg
    22201 aagcaaatat ctttacgcct cgctccgccg caaacttagc tcatagctca taacaagctc
    22261 accgttcacc gttcaccgct caccgctcac cgctcgctgc tcaccgctat cagagtcctg
    22321 tattaaacaa cgagtccacg ccgtgcatct cggtcgaatc gcccattagc gaatctggaa
    22381 tcgatgtcca gcattcgtat tttttcgtga gtttcttttt gggttcagga aattttgccc
    22441 ggtatttttt caatgattcg tctttgatca ctttttgcat aaaaatacca aacacaggca
    22501 gggcagagta gccgccttcg cccaattcaa atccgtttcg gaaatgtatg catcgttgtt
    22561 cgccgccaac ccaggcgccg ccaacaagtt tgtgcgttac tcctacaaac catccatcgc
    22621 tgaaattgga cgaagttccg gtcttgccgc cgaaatctgt ttcataacga aacaagtcat
    22681 attttcgaag cgcctgcgtc gttgagccac tttccttcat gccggcctta agcatttcct
    22741 gcatcatgac cgctgtttcg taatcgacaa ctttttttcg atggggcttg tgttgccaga
    22801 taatattacc atcgcggtct tcgatgcggg taacaagtac cggggcttca tatgccccgt
    22861 cggcaatgat ggtggcgtag gaattcacca gatcgagcaa ttttacatcg gatgaaccca
    22921 gacacataga gggaactttt tcaagcttac tgcggattcc aaggcggtat gcgtactcca
    22981 tcactttgtc gaggccgact tcacgcgcta ccgcaaccgc aatgctattg atggaatgag
    23041 caaatgcata gcgcagtgta acaggaattc cgaagtgttt tccggttaca ttgtgaggcc
    23101 gccataccat atctttcccg ttttctttgt atttccattc aatgaactga tctcttcgtg
    23161 tgtcgcacgg agccatgcct gcagccattg cggctgtgta aacaaaaagc ttgaatgtcg
    23221 atccgggctg gcgttgtgcc tgactcacat gatcgtattt gaaaagctga taattcagat
    23281 cgccgaccca tgcgcgtaca tatctgttct gtggctcaat agcaacaaag ccgatgtgca
    23341 aaagccgttt tgtataactg atggaatccc atggactcat ggttgtgtca acaacgccta
    23401 gtttcggatc gtaaatcttc atggcgatgg gttgtttcat cagagtcatg gccgagtctt
    23461 cgctgcctgc ttcttttctg tattgtttgt accgtgggca ttcgagcgca ttgttgtaga
    23521 tgaaatccgg aatttcgttg taattgatgt cgcgccaagg gttttcattg ccccaatgac
    23581 gttcgaattc gcgttgaagg aaggtcattt gtttgttcac ggcttcttct gcgtaagtct
    23641 gaatgcgact gtcaagtgtt gtgtatattt tcaatcccga actgtaaatg tcataaccat
    23701 gagtcctgca ccaggtgccg attgatgcag caacggcatt gcggaaatag ggcgcttcgc
    23761 catccgaaac atcttctact ttatatttca aacccaatgg cagagctgaa agtgaatcat
    23821 tttgagttat tgaaatcagc ttgtggtttt gcatgatgtt taaaacaaca tttctgcgtt
    23881 ttgtacagtt ttttggattt ttcagcggat tgtaggtgct tggggctttc agcatcccta
    23941 tcagcatggc aatctgctcg gctttcagtt cttttggcgt tgtatgaaaa tacactcttg
    24001 atgcggtttt gatgccaaat gtattgtttc cgaaatccac agtattaaga tacatggcca
    24061 gcacttcttc ttttgagtag tacatttcga ttttgaaagc accaatccat tctttcagtt
    24121 tcataacgac tactcgcaga tagggaatat gcccgcacag accttttgag taggtcgatc
    24181 tggttttgta catgtttttg ataagctgct gcgagatggt gctggcgccg cggtagttgc
    24241 ccgaaaacca gtctttgaca gcagaaaaca gcccgataaa atcaatccct gaatgtgaat
    24301 aatagcgttc gtcttcggtg gctataagcg tttctacaag caatggcgat agctcatcat
    24361 attcaaccgg agttctgttc tcacggtaaa aacgtcccag catttttcca tcgtcactgt
    24421 atatttcaga tgcgatgctt tgttgcgggt tttttatatc cgatattcgc ggcgatgatc
    24481 caaaaagcca cagaaagttg atgttgatca gaaacagtaa aagtacgaat gcgagaaatg
    24541 aaaacatcat aaaacgcatt acgcgtatat accatttccg cttcttacga agttcttttc
    24601 tgtgttgtct ccaatttttg aagcgtgaaa acacaatagt ggaattttcg gcaaaggtat
    24661 ttgaatttca cgcctgagat tcttttcaat catttttttt ggtgtataat aaaagccggt
    24721 acatcttttc aaatgtaccg gctctgtatg aattttaaat ttgtttattc aatggttaac
    24781 ctaagtgttt tcatttcctt gctttcaacg atgataatca tatataatcc tgaattcatg
    24841 ccggaaagat caaggttgaa atgcgcttca tttgtgctga tttcgcgcac aacctgaccg
    24901 gtcggactga ttactctgat ggtgcgttct gcagcatttc caaattgcaa tgtggcgttt
    24961 tcgttggcag gattgggata taataaaatg tttgtttcat tttcttcaat accaacgccg
    25021 gtttctgttg taaaactcca gatggcagaa tagggcgagt agattgctcc gtcgctgctg
    25081 cgcactttcc agaaatagct ggtgttgttg ctcagtccgg tgatggtaac ctgcgatacc
    25141 gttacaatag catgtgttgg attcgtgaaa ttgatatccg tgtcatagta gtattcatac
    25201 atcgatgcac ctgaaatgtc gctccagtca agcagaagat tggtcactgc ctgattgctg
    25261 gctccgttgg cgggggtgat aagtgtcggt gctgtcagcg ttgcagttga aaagctccaa
    25321 ggtgaacacc atgccgaaaa gttggttcca tcactgctgc gcacacgcca gtagtaagtt
    25381 gtattatatg ccagtgatga aacagccgca ttggaaattg tcggagaacc gctcgcaata
    25441 ggacttccaa acatcacatc atcatcgagt tcatattcgt agaacgttgc tgcagctgca
    25501 tcctgccagt cgagcgtaag gctgagattc tgatctgttg cgccgttggc cgggctgatc
    25561 aataccggaa ctgccaaacc gctggtggca aatgtccagg ccgttgacca cgggctgtag
    25621 ccgctgccgt tgttggcgcg tactttccag taataggttg ttccgggcag caatggtgcg
    25681 gtaacctgcg aagttgtaac tgctgagccg cttacagcat tgttgaatga tgcgttgtca
    25741 gcatacatgt attcataaaa cacagctgtt gcatacgttt gccaggtcat attcactgtc
    25801 gaagtcgcga tgcctgtggt tccgtcagcc gggctgctca gcacaaccgg agtggtaagc
    25861 tgatacagtg ttgtgaaact ccatggggtg ctccagtccg aagtgtcggc cggatggcag
    25921 gtgcgaacgc gccagtaata agtttgtcca tagctcagat tgcttaaccc ggcctgcgat
    25981 gtagttcccg tgccggtagg cgtggcgctg ctgaaggtcg ggtcggccga atattcatac
    26041 tgataaaggg tgattcctga aacaacactc cagtcgatgg tgggattcaa agacactcct
    26101 gttgcagcat ttgccggact tacatgaact ggtttaccat ctgttgtggt gaaggaccac
    26161 accgaagacc aaattgaagt atccgctgaa tgccttgatc gggcgcgcca gtaataagtt
    26221 gtagagaaca ggatatttcc aggggtataa cttgaatacc ctgaaagagt ggtttgaacc
    26281 tgatgaagcg ggctgttgaa tgaagctgtg gtatcgtatt caacatcata gtgcgtaatg
    26341 ccattgatgg ctgcccagtc cagagttgta tttacgggtt gtgccgtagc tccgtttgca
    26401 ggactggtaa gtgccatgct attcatggtg gtgaaactcc aaatggctga ccatgtcgat
    26461 gtgtcggccg aatgacgcga acgtgccctc cagtaatagg tggtgccgaa cagcatgttt
    26521 ccgggagtat agctggaata tccggttaag gtcgttttca cctgatgaag cggactgttg
    26581 aaagacgcag tggtgtcata ttccacatca tagtgagtga taccgcttac tgcaccataa
    26641 tcaagagtaa catctacatt ttgattgatg gcgccgtttg agggcgatgt caaggtcatt
    26701 gtattgtggg tggtgaaact ccaaatggct gaccatgtgg atgtgtcggc cgaatgacgc
    26761 gaacgtgccc tccagtaata ggtggtgccg aacagcatgt ttccgggagt atagctggaa
    26821 tatccggtta aggtcgtttt cacctgatga agcggactgt tgaaagacgc agtggtgtca
    26881 tattccacat catagtgagt gataccgctt actgcaccat aatcaagagt aacatctaca
    26941 ttttgattga tggcgccgtt tgagggcgat gtcaaggtca ttgtattgtg ggtcgtgaaa
    27001 ttccaggtgg cgctccacga cgttgtgtct gatgcatgac gtgccctgac tctccagtaa
    27061 tatttctggc cgaagcgcag atttgaagga tagtaatatg aataggaact gctctgtgga
    27121 acgagctggt ggagcggact cgagaatgat gcggttgtgt ccagttcaac atcgtaatag
    27181 gtgatgccat tcaccacacc ccagtcgaga tatgggttta catcctggcc tgtagcgccg
    27241 ttcgatggag ccacaaggta aacattgtcc atggtggtga aattccatgt ggcgctccat
    27301 gaggttgtat cggtgctgtg ccgtgcccgc actctccagt aatatttctg gccgaagcgc
    27361 agatttgatg gatagtaata tgaataggag ctgctttgtg gaacgagctg gtggagcgga
    27421 ctcgagaatg aagcggttgt gtcaagttca acatcgtaat aggtgatgcc attcaccaca
    27481 ccccagtcga gatatgggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    27601 gttcaacatc gtaataggtg atgccattca ccacacccca gtcgagatat gggtttacat
    27661 cctggcctgt agcgccgttc gatggagcca caaggtaaac attgtccatg gtggtgaaat
    27721 tccatgtagt gctccacgtt gtcgtatcaa cattatgccg tgctctcatt ctccagtaat
    27781 atttctggcc gaagcgcaga ttggccggat agtaatatga ataggagctg ctctgtgaaa
    27841 cgagctggtg gagcggactt gagaatgatg cggttgtatc gatttcaaca tcgtaatagg
    27901 taataccatc cactatgctc cagtcgagat atgggttcac atcctggccg gtagctccgt
    27961 ttgaaggaga aacgagactt acaccatcca tggtggtgaa catgtaaatc acagaccagt
    28021 cgctgctgtc ggtggcactg cgtgcgcgaa ccgaccagta ataggttgtg ttgaatttca
    28081 actcagaagt atagaaatat gagtttgagc cagtcgaatt ggcggtggct ccggcaagtg
    28141 tcggacttgt actcagctta tattgataat aggttgctcc ggttgaggtc ccccagtcga
    28201 gcaggacatt cacggtctga ccaacggaac cgtgcgaagg agctaccatc gaaggtgtga
    28261 ccaataatgc aaaggatggt gcatgcaaca gcaaagcagc gacagctgca agtaaaagct
    28321 ttttcataac agataaagga ttttgattct attcttcgtg attttccccg tgcgaacaag
    28381 ctttgtgctt gagttttgtg tcggccggat ctccttcaca taagtagcca atttgatgaa
    28441 tatattcttt aatggcatca ggaccggtca ttttggtatt ataggtaata tcaacaattt
    28501 ttgtatccag atgggcaata gctgaaatca caccgcgttt tttgggcagt tcggtttcga
    28561 gcaatgcttt gccattggcg caatggaaaa ggactttgat gctgaataca gcttgtggtg
    28621 attgttcggt aggttctgaa tcgcgggttg cagtattctg tgcatttgcg ccagaaacag
    28681 tcaacacaaa caacaaaaga gcgaagattc tgaagaagtt tttcatgtgg ttttgaattt
    28741 tatttttttg tttcagacaa aatttttgcg aatttattgt gttgcaaata tatgcatttt
    28801 taaagaatgc gtttgcatca gcaatttaat tcgaccggcg cccctatcaa atcttttgcc
    28861 ataatatttt gaagcttcat gaaaaaaatg taggtttgca gtcagtttaa cgaaatcagt
    28921 agggtatgaa aaaaattatt gccattcgcc acgaaaacaa atatgagatg gaacgccgcg
    28981 ttccgattat tcccgatcat gtaggatcgc tgatcaacaa aggatacgaa attcatgtgc
    29041 aatcttctgc aaaacgtgtt ttttcggatg atgaataccg gaatgccggg gcgattgtca
    29101 ataacgatat atctgatgcc gatatcattt ttggcgtgaa ggaaattcct atgcaggacc
    29161 tgatgcctgg caaaacgtat atgttttttt cgcatgttat taaagggcag ccctataata
    29221 tgcctatgct caagcgtctc atggagctta aatgcaacct tatcgattat gaaaaagtaa
    29281 ccgatgaatt caataagcgc gtaattttct ttggaaaata tgcaggtctg gctggcatga
    29341 tcaatacact ttggtcggta ggacagcgtt atcagaagtt ggggattgaa acgccttttg
    29401 tcaagttgcg tcagtcatat acctataatt ctttatccga agcaaaaaaa gccattagta
    29461 aagttggcta tgaaatcatg aaacatggct tgccgaaaga gttgcagcca ttcactattg
    29521 gaataaccgg ttatggaaat gtgagtatgg gggctcagga aattttacat ctgctgcctg
    29581 tgaaagaaat tttccccgaa gacctacctt tgctgaaaga agatatttcc gatggcaaag
    29641 ttgtgtacaa ggttgttttt aaagagcaag acatggtgaa acatgtgggt gaagaacgtc
    29701 catttgattt gcaacattat taccagcatc ccgaagaata taaatcccgt tttttcgatt
    29761 atctcgaaaa catgagcgta ttggttcatg gttcttattg ggatgcgcgt tatccgcgtg
    29821 tggtttcaaa aaatgaaatt gcaagactgt attcaaaatc aaatccgaag ctggtggttg
    29881 tgggtgatat ttcatgcgac ccggatgggg gtgttgaatt tacacataaa ggtactgaaa
    29941 tcagcgatcc tgtatttgtt tacaatcctg ctacaggtat tccagccatg ggtttcgacg
    30001 gcgacggcgt ttgcgtcatg tcggtcgata ttcttccaag cgagctcccg cgcgatgctt
    30061 cacgtggttt ttccgatgca ttgcggcctt ttgttgaatc cattgtagaa tgcgatttca
    30121 ataattcata cgagaatctt accattgctc ctgccatcaa acgcgcactg attctgcata
    30181 acggagaact gaccaaaccg tttgaatata tttcaaaata tttttaatgc cacaggaagc
    30241 tccacgtaga tttgtaagcg taatcggtcc aagctactgt aaagtatcgg atcctattta
    30301 tgcatttgcc cgtgaactgg gcactaagct tgtcgatcat ggctttactg ttttttcggg
    30361 tggccgccgg ggcattatgg aagcggtttt ccggggcgca catgaatccg ggaattattc
    30421 ttttggcgct actgttggca ttttgcccga cagtaaccgt cattcggcca accgttatac
    30481 cgatatagct gtagcaactg gtattggctt tgcacgcaac agtattgtgg cgcagagtgg
    30541 cgatgtgatt attgcgctgg gcgggggatc tggcacttta tctgaaattg cttttgcctg
    30601 gcagtatggt aaaaaaatca ttgctgcttc aaacttcgat ggctggtcgc gcaatctgag
    30661 cggtaagaaa ctcgatcacc gccgccgcga caccatcatt caggcaaaga cggtggatga
    30721 aatcattgcg cagctgaata agttgctggt ggtgaaatag gtgtcttttt taattgacat
    30781 atgggcatct tatatcgatt gaaataatat taatttttgt cttatttatt taagaaaaaa
    30841 tctctaaatt tgctttaatt aactaagagt ctgtctaaaa atgatgtcag agttgttttt
    30901 agacattctc taatctccat ttcaaacaat tcaatgcatg caaaaaaagg ccatccacac
    30961 agagcggaca gccttaaaaa agtaaaagct tcaaaaaatt atttcgggga atagtatgtt
    31021 ctgataagat caaatgtgct gtcggcgaga aacaaaggaa tattcagaac accatcgtcc
    31081 agtttcaggt ttcgcaatga gtagcgaatg gaaattccgg gagaataggt tttccgataa
    31141 acagccagac ttttagagcg gatgttttca tctgatttta cttcaaccgg gataatttca
    31201 ttttcatatt gaatcagaaa atctatttcc gcctgattcc ccgaggtcca gtaacgaggc
    31261 actccttcaa actgttgaat caggctttga agtatatagg tttcagttaa tgctcctttg
    31321 aactcagtaa acatcctgtt gccttcagta attgcaatcg gattgagcaa tgacatccgg
    31381 cgcagcagcc caacatcgtt catgtacact ttgaatgaac tcagatcatc gtaggctgac
    31441 agcggcatac ggggagctgt cactttgttg attttgtaaa tcaatcctgc attttttagc
    31501 cattccagtg cattttcata ttctcttgct cgggcaccgg gttttacact ctgatagaga
    31561 aattttttat tttctctgga taattgtgaa ggtagagatg tccagatata atttattttg
    31621 tgaaaatcat tgacagacgc atgttttgta aaatcaagtg catatgcatt cagtatattt
    31681 tgcaatgacg tctgtgtttc gttgacatcc tgattttgaa gcaacgaaac aactgcctcc
    31741 ggcattccac cggaaataaa gaagcgttta aattgctcca gcagacgatt gaaaaaaatt
    31801 tcaggtattg gttctggttt actaatgtta tttactactg gaagcaaatc gggtgaggca
    31861 gccgataaaa attccttgaa gctgatggga tagagtgtca taaaatctac ttttcctacc
    31921 ggaaatgagc tgccgctggt cagggcaaca ccaagaagtg agcctgcaga aacaatacaa
    31981 tatcccggca ctttttcgta aaaatacttg agcgcgttca gtgcttcatt gcattcctga
    32041 atttcgtcga aaataataag ggtcgtttct tcttctattg attttccgta aaccagcgac
    32101 agattcccga ttattctttt cgcatctctt gtgcctgtaa aaaactgctt taattccggc
    32161 tgctcctcaa aattaaaata ggcaacctgc ttgaattgtt tttttccgaa ttcatttaaa
    32221 agccatgttt tgcctacctg ccttgctccc tgtaggatta atggcttgcg ttttggctta
    32281 ttctgccatt caattaattg attaatactt cgacgaaaaa tttccataat cacgcaatta
    32341 atatgaaaaa tgtgaattta atcacgttat acttactaag cattggcaaa gttacacttt
    32401 tataaattgc tgctaacatt ttgaatgttg aaattttgca tgaaaacgca ttgatattgt
    32461 gcaaattttg taaacaaaaa aggccatcca cagcggacgg cctttttcga aatgcaatta
    32521 tcttagtgga aagaaagaat tgatttctta atgatagcaa ctgcttccat aatctgatct
    32581 tcggtaatgg tcaaaggcgg agcgaagcgg atgatatgac cgtgggttgg tttggcgatc
    32641 agaccgtttt cggccatttt caaacaaacg tcccatgctt cttttccgtt cataggctta
    32701 atgatcacgg cattgagcag acctttacca cgaacgagtt cgatcatcgg gctgtcaatt
    32761 ttgcggattt catcgcggaa aatctttccg agtctttctg cattttcgac cagtttttct
    32821 tctttgatta cttcgagagc tgccatggca acgcgggctg caatcgggtt tccgccaaag
    32881 gttgaaccat gttcaccggg tttgatgttg agcatgatgt tgtcgttggc caatacggct
    32941 gacacaggga atgcgccacc accaagggct ttgccaagaa taaccatgtc gggtttgata
    33001 ccttcatgat caacacacag catttttcct gtacgtgcaa taccagtctg aacctcatct
    33061 gcaacgaaaa gcacattgtg ttttttgcag aggtcgtatg cttttttcag atatccttcg
    33121 tccggaacaa agactccggc ttcgccttga atgggctcaa caatgaatcc ggcaatattc
    33181 ggatcctgct gcagttcttt ttcaagagca gccaggtcgt tgtacggaat ctgaataaaa
    33241 ccaggtgtga acggaccgta atcatcataa gctgaaggat catccgagaa gctgacgatg
    33301 gtggttgtac ggccatggaa gttgcctttg gccacaatga tttttgcctc gtaacgtttg
    33361 ataccttttg tcagataagc ccatttacgg gtgagtttga tggctgtttc aacagcttcg
    33421 gcacctgtgt tcatgggtaa taccttgtca taaccgaaat attcggtgac atatttttcg
    33481 taaacgccca gcgaatcgtt gaaaaacgca cgcgatgtca gggtgagctt tttggcctga
    33541 tcaatcaggg ctcccacgat ttttggatga caatggcctt gatttactgc tgagtaagct
    33601 gaaagaaaat caaagtactt tttgccttcc acgtcccaga caaatacacc ttctccgcgt
    33661 tcgagaacca cgggaagcgg atgataattg tgcgcaccat aatggtgttc caattccata
    33721 agctgcgctg aagaaagacg ttctgccata atttttttct cctttttttt aatgttaatg
    33781 caacaaagat actaatgtta agcacacgac aatatgaacg gaaaaacaat tttttttcga
    33841 aggatatcag aaaatcacta taagattgtg cccgtctggc ttataggggc tgtctcaaaa
    33901 aaacggccgt tacgacattc caccgttccg gccggctcgg ggaggattcc tcaagattat
    33961 aaaccattgt gaaatttatt gtaggtcaaa atgtttttta aatctttaaa tggctgatgc
    34021 cagaggcatc acatgtctat agaaaatgac caaaaaaaaa tcacgatccg ccggctggtg
    34081 gatcgaatgt aaacggcttc ctgcgcgaaa cagcttgaat aacattcgac ccgccagctg
    34141 gcgggtcgta gtgtgtttta actatttcta cagacatgcg actcctccgg agtcatccaa
    34201 acaaatgaac tattgtatat ttcctttcgg tattattaaa gattaacgat ttccatcgtc
    34261 tgccatgttt cgtcattatc agcacatgtc tggcggatat attaccgtcg taacaataac
    34321 aaaaacaacg gttccatttc gctgcggcga acttcagctc cttcgtcaca tcggacgatg
    34381 gaatgttgtt tttgctctaa gtttgatttt gagacagcct tagtcctatt tacaaaactt
    34441 cgtttcttct ttgatggatt tgattccgca ttcttcggcc ttcagccagt cgcggcatgc
    34501 attgttgcga tcgttgcgct ctttgtacat acgtccgcga ttgacatatg cctgtccgaa
    34561 tgtggaatct gtttctatgg cttttgaata atactcaaaa gccgaatcca attgattcag
    34621 tccaaaataa gatgttccta tgtaaaaata ggtttccgga tcctgatcat cgtattcaag
    34681 cgatttatgc aatgtctcta tggccttgtc aaattgtctt ttataattca ggtcaatgcc
    34741 ctgcatcttg aggtcaatgg ctttgcgttt tgcatcctga catcccggga ataaaagaag
    34801 tattgtaaag accgctgtca gtgcgcggaa tgaaaaaagc tgaacattca tatcaattgc
    34861 ctaaagtggt tgtcaaagtt agataaattc ccgagacaat attgtcacgg tattgtgcgc
    34921 caagagccat tacattggga cctgtggccg aaagcagtct ggagtaacgg ataaactccg
    34981 atcggtaatt cactgtccag gtctgacctc cgtcaagtgt gcgcaatatg cagccggtca
    35041 tttcatcaaa gctgtcgccg cagttcacca cagtgggaca cgcaatggcg gcaaagccgt
    35101 tgttggcgtc aagaaaatgt acggcctgtg tctgagccag ccctgcgtac ggataaatgt
    35161 tggtcagaat gaatttctgt gtccaggaat ttccgttatc ggcactcatc agtacacatc
    35221 cgtttttgcc tcctgcaaac caatgtccgt ccgggttttt ataaagacaa tacagcgttt
    35281 gtgttactgt gctgagcaat gtccagtttg aaccaagatc atccgaaaca cggatttcat
    35341 tcccaaaaat tccgattaat gtgttttcat cgtagttctg aatatcagtt gcattcaggg
    35401 tgctgttgat ggtccagttt agcccgccgt tttgacttac tctgatgcca tcggcaccaa
    35461 atgcatataa ggtgtctgcg ctcagaattc tgattttact tgcatttcca gccatgtttt
    35521 gccatgtcca ggatccaccg tactgttgtc ctgaaccaaa ctccacattt gaaagatagt
    35581 tgcgcagtgc ataggatttt tcgcccgatt gggctgccga taaaatgtat ccgttttgtg
    35641 agctgtgcat agtccagttc aatcctgcgt tctgtgaggt gaaaatatag gtgtattccg
    35701 tattgagctt cagactcgca taacatgaaa cggtatctcc tttcacatac agaaatcctg
    35761 tactgacttc gcagtattca cccggcaatg cctgcggtgc attgctgtac ggacgctgta
    35821 ccagcaaccg cttgctccag ttactctgtc cttcgttcaa ttcaaattca ttgtcagttg
    35881 tatcatcatc gtctttgcac gatatcgcga aaagcaatgt cagagccagt acagaaagaa
    35941 atattatttt tttcatgatt gaaatgttga gaggatttcc ctcattttat ttactttttc
    36001 atcaagcaat gttgttgttt tggcttccac cagcaggcgc agatagggct cagtgttcga
    36061 tggccggatg ttgtaccacc aatcgggaaa ttccacccga tagccgtcga agtcaagaaa
    36121 cagagtagga gaggcctccg attcgaaata atttttcact gcatccatgg ccttttgttt
    36181 ctccgcgatg cggaaattga tttctcctga gttttcgtat ttgcggatgc ggcctacaac
    36241 agctgatgca gtcagtcctt cgcgcttgaa ttgcgcaaac agatttatga tcagaatgga
    36301 cgccagcatg ccggaatcgc tgtaataaaa gtccttgaaa taataatgtc cggccagctc
    36361 gccgccatag attccatcga tttcacgcag catgttggct gcataggcac gtcccacacg
    36421 ccagattttc atctcggcac ccattggaac cagatattcg gccactgatt tcgatgtgcg
    36481 tatatcctga atcacttttc ccttcaatcc tttttcttca agaaaataat gtccgagcag
    36541 acctattagc aaatcaggcg gaatgaaggt tccaagctca tcggtaaaca tcacgcggtc
    36601 ggcgtcgccg tcgaaaataa cgcccagatc ggcaccggtt tcctgcactt tctgttgcag
    36661 ctgaacgatg tttttcatgt cgagcggatt gggctcgtgg ttcggaaagc ggccatcggg
    36721 ctcatcaaac agataggtcg ggtgtgaacc gaaaatgtct ttcactatta tagaggacat
    36781 gccgttgctg caatccatca cgattttcag atttttgata tcgtttttat aaggcagcat
    36841 aaaggcaaca taatctttca gaaagtcaaa gctgatcacg tttccggggg ttcccgacgg
    36901 agcaggaatt tcttttattt tctgctcaag aatgttcaga ccggtgtcat atccaactgg
    36961 tattgcgttg gcggccgata tcttgagccc gttgtattca gccggattgt ggctggccgt
    37021 aatcatcacc gaggcatcga atccaaattt cgcggtgccc cagtaaacca ttggcgtagt
    37081 gcaaagccct gcatccgata cttcgcagcc ggcatcggtg aggcctttca gtaattgccg
    37141 gtgaatttcg ggggatgatt cgcgcacgtc gcgtcccacc agccaacgtt ttgcattgag
    37201 aactgaaggc agaaaaaagc ccattctata tacatcttcc gaactaaaat cttttccgta
    37261 aactccacgg atgtcgtagg catgaaatgc tcccattgtt ttttttattt caaagataag
    37321 caataattgg tgaaatgcaa caatgctgtt tgctgaacca gggtcaacgc attaaaacat
    37381 tcaaaaaacc acatgaaccg tagttacaca gagttccgca gaggtatcac agtgtttcac
    37441 agagtaacga aaaaacaaaa gccgattgaa aattacacaa cgcagttgtg ttaatcaatt
    37501 acagcatatc gaattctgaa agctcgcttt caaattcact ttactgtaat tgatatcggc
    37561 ttttgacaga ttaaatctac tctgtgcgac tctgtgtgta ctcaatgaaa ctctgtgtta
    37621 caaagattga tattgcatgc ttgaagctgg cattgggtgt ttcaatcaga agtatttaaa
    37681 ggcatttaaa tgcgttgccc ttgttggccc ggacagaaac gcacacacat ttcgaataat
    37741 tttatatttt agccgtgatt taggcaaaaa aatgtacgaa ctctccaacc atctgggcaa
    37801 tgtgctcacc actgtgtcag acataaaact gaccgccgac ggcaataccg atggtgtggt
    37861 tgattcttat gctgcagtaa tcaccagcgc tcaggactat tataccaaat tgaaattgca
    37921 gttagtccaa agacgcactg caatttctta tttggataat gccgttgaaa catgctgtag
    37981 cgctttgcaa tttggcctga aatataaatc aacacggtat tatccgtttg gctcgcttga
    38041 accgggaaga acctattctt cggacaacta ccgtttcgga ttcaatggtc aggagatgga
    38101 taacgagatt tctggccaaa cgggaacgca tacaacggcg atgttttggg agtatgatag
    38161 caggttgggg agaaggtgga atgttgaccc tctatcgaaa ttagctgctg gatggagtcc
    38221 ctatcgtgca tttttttgta atccgatact ttatagtgac ccgtttggat tgtttgaaac
    38281 gcgaaaagaa gcaagacaat acagaagaga aaatggctta agaggaaaaa ttaatta
//
LOCUS       DAOPKE010000016        38299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1132_length_38299_cov_13.605664, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000016 DAOPKE010000000
VERSION     DAOPKE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_38299_cov_13.605664"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /locus_tag="PKY63_03660"
     CDS             complement(<1..2366)
                     /locus_tag="PKY63_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPE99743.1"
                     /translation="MNTYNAVESSLGFSEIRKKLQSVCFTPTGLERAAGVFFTSDTDK
                     IQQSLLLTAEMKYILEYLGGVPALGFEDFREELSRLKTQGIAVEIPTLQAIAFILEKC
                     DDLKQLFRQQDDKTPLLKARLDDHPFDFSLAVRINEILDENGDIRSSASERLSEIRKS
                     ITGQRKKIERNIHAMLKSLKEKGVVEEEVNLSIRGGRLVIPVHASKKRMIKGVVLDES
                     ATGQTVFVEPIEIFELNNEVQDLEFEEKREIHHILTSLADRIRPSVPELHSHIDFLGE
                     MDFIRGKAQLAVEQNAVVPVIPQQASIVIMQGRHPVLEESLLKQGKKVVPLDIELLPA
                     QRLMIISGPNAGGKSVAMKTTGLLQYMFQCGFLVPAAEGTMLYPFEAILADIGDQQSI
                     ENDLSTYSSHLLNMKKFMERGNSNTMILIDEFGSGTEPESGGAIAEAVLEILNAKGVY
                     GVITTHYFNLKMFATNHPGVINGAMLFDNDLLKPMYKLQTGKPGSSFALEIAASIGFP
                     QALIEEASSKIGTARIEMEKMIQDLDNEKLKLEDRRRQIEVAEDFVSELIEKYNKLNK
                     SIVEKRDRIMSKAESEAKNILSEANALIEKTIRDIKQEQAAKDKVRVIRTEFEKKASS
                     LKTTKEKPIEPLPLKKTIHTDDAPKKPLAVGDSVRLDDGIETGTVIEIKNKKVRVQFE
                     LASLTVDSARLSVVATQKQKQHQPKKKVTLISEKKETVYQLDYRGMSAIDAIMELDKF
                     LDDALLTGVRSFSVLHGKGFGILRKEIRNHLRQYKDMLEFTDAPLQMGG"
     gene            complement(2480..3223)
                     /locus_tag="PKY63_03665"
     CDS             complement(2480..3223)
                     /locus_tag="PKY63_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPE99744.1"
                     /translation="MYHQILGVPESASIQEIKKAYRTLALKFHPDKNPAPDASDQFIR
                     VTEAYEILIGERCRPTSRTSSPAYSADDLARAAREKARQYARMKYEEFKRKNDAFDMP
                     MHSILWPKWVNFIILAFCFVMIADHWLPTRDVQRPVGRDENGKFSPDLVDLSLMPGLA
                     GETMTDSVITIHYTPVFGFVESCEMMGRRYESSDSANQIIVVVYIIVVLTLIILFGKT
                     KKLENKLLVKAFTAMLLLIYLLIFFSHFF"
     gene            complement(3241..4305)
                     /locus_tag="PKY63_03670"
     CDS             complement(3241..4305)
                     /locus_tag="PKY63_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPE99745.1"
                     /translation="MAGGLGTRFWPMSRTLKPKQFIDVLGMGKTLIQLTFDRLLKICP
                     AENILVVTNENYVELVKKQLPLINPGNVLSEPSRRNTAPCIAYAAYRINSFDPDATMI
                     VCPSDHIIFDEDAFIEHLRSATVASQKNNWLMTLGIMPTRPDTGYGYIQFLDGQKVEG
                     DSRIKKVKTFTEKPQLELAKEFIKSGDFLWNSGIFIWTTKSIIDALQKHVPDMSALFA
                     KGNKIYRTVDEADFIFQTYMLCKNISIDYAIMEKASNVYVVASDFGWSDLGTWGSLYE
                     NRQRDQNGNAIVGKNVMMYDSKNCLVHMPQEKMVLLQGLNDYIVVESDHILMVVRKED
                     EQNIRQYVNDVMIEKGEQFT"
     gene            4562..5638
                     /locus_tag="PKY63_03675"
     CDS             4562..5638
                     /locus_tag="PKY63_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99746.1"
                     /translation="MNKSLVIFLLFAAALAPANLMAQDTIKPAKSFLKNTEISGQWFI
                     SGNYENHDSLYQIELKRGYFTIESHLTENLTARYTQDITLDTEGDDAGNVEMRLKYLY
                     LQQKIHFLPKFQQGSFSIGMVPRPWIEFEQKINSYRVMGPMFLEKTNVLSSADFGFIY
                     EGLIGGEIDKQYQKEVNAAYPGKYGSFSFGLYNGGGYHDVERNNNKTFEARLTLRPLP
                     AFMPGLQFGYAYANGKANIANDADFNMNVIYLSSESKYHVFMAQYYSGVGDYAGLYTD
                     TLFNSYKNSGYALFGEFKIPQTKFSVVGRYDSFKSENEFSLTRINTGIAYRFLKNKLV
                     LGAEYWDKNGDIRRMFELALEIRF"
     gene            complement(6086..6790)
                     /locus_tag="PKY63_03680"
     CDS             complement(6086..6790)
                     /locus_tag="PKY63_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPE99747.1"
                     /translation="MEEYFSHILFLDIETVPAMPSYNKLSDRMKHLWDKKAESMKRAE
                     NDTPETLYEKAGIFAEFGKIICISTGVIAGGKAHIKSFFGHDEKELLNEFADYLRDLP
                     SRGVRLLCAHNGKEFDFPWICRRMMINGIRIPDMLNIQGKKPWEIAHIDTMELWKFGD
                     YKNFTSLDLLTELFDIPTPKDDINGADVARVYYEEDDLIRIMQYCQKDVVALIQLLRR
                     LNYMPLIPEVNVMYGA"
     gene            6842..8131
                     /locus_tag="PKY63_03685"
     CDS             6842..8131
                     /locus_tag="PKY63_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99748.1"
                     /translation="MRYKWPILFLLTLIFVSSCREETYEPPFIQMFSPSENGMAQTGD
                     TLQVKGSISSAIPITYIKIVLLTSTYIQSCPATTIYPNTTNYNLNTEYIISNNNLLSG
                     THYLFIEAGNEYASGNRFIKLTISGIPKESKALFAFCHETANDHVKIFKVDSLLNYTL
                     FKQLPDDFQEGAVNSQDQLIYSMGLYEGNLYSLDASDGNILHQVNAIINPPFPYFESL
                     YWGNNLLVVGYYDGRIEGYFGNGNLKFVYSIENFRLHKVSYDGLYLLSVLEYYTGTEY
                     CAGAIYEFSGLLKDMIFVNYEIVNFYRASGNDVVLFCNDALQPSVKAFNTYTMVITHI
                     KNLPAGTIQSVTQIDDDKFLICHSNGFLLYSFDNNSYAETGSGSTSGEIVYDEPDNII
                     YLSEGKNISAYSYPTLQHIGTVILSDSIKDLEILYNR"
     gene            8128..8808
                     /gene="mnmD"
                     /locus_tag="PKY63_03690"
     CDS             8128..8808
                     /gene="mnmD"
                     /locus_tag="PKY63_03690"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPE99749.1"
                     /translation="MKTKKLIITEDGSHTLFIPELNEHYHSIHGAVAESRHVFIEAGL
                     KPKIIENKKLNVLEVGLGTGLNAALALVAAEENQIQLNYFAVEPYPISTEDMMQLNYS
                     KVSESTRMNEVIPAIHAASRDNEVHIGPYFIFHYFEKRIQEIEFENNFFDLIFFDAFS
                     PGVQPEMWTMDVFQKIAAAMKPGAVLVTYVAKGEVRRTMKACGLSIEKLPGFGGKREM
                     TRATKNVF"
     gene            complement(8878..10194)
                     /locus_tag="PKY63_03695"
     CDS             complement(8878..10194)
                     /locus_tag="PKY63_03695"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99750.1"
                     /translation="MKTLISAFILFFFTSTMLFSQVSTAEKSVNNEKKFFPAKADPKT
                     KGPNEKVFCFPFGAYSNVPTGPSYFYLSNPDSIYLIDSANTEIYAATWGNDTWYGADY
                     SNMTLVNIDTATGAITTIGSMGVVMNGLAYDPVSDLLYGVSSTGLYSIDTLTGTVTLI
                     GEANVASSTFINLACSPSGKLYSVNMNDDILYSMDKTTGAATAIGSGIGLALSYAQDM
                     EYDYNNNTLYLAAYIGSGAGGLYVVDTLTGTVSTCLGGFNNAEVCGMAIPLYTISTYS
                     VDFSVLNGHGSLSASANGLAISSGDFITEGDSVEFIAVPDSGYVVKEWIYNSAFISGN
                     TTNNFSIDGLIENAVVTVEFVSTAGLTDFDKNEFLVFPNPAIDFISINVEENCVMNLW
                     TAAGSLVISRVLQPGNQSLNVSDLPEGVYYITLTGDDIKRATFVVE"
     gene            complement(10675..12399)
                     /locus_tag="PKY63_03700"
     CDS             complement(10675..12399)
                     /locus_tag="PKY63_03700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE99751.1"
                     /translation="MLRITFLLLFLSVVPAFACTNLIITKGASKDGSVMITYAADSHR
                     RYGYLFHYPAADHKPGDMVDLFHYETGKLLGQIPEVAHTYNVVGLMNEHQVAIGETTF
                     GGLEQLQSQPDALPDYGSLIKLALQRAKTAREAIMIIAELVEKYGYASSGESFSISDK
                     NEAWIMEIIGKGTYEKGAVWVAMRIPDGYVSAHANQARITTFPFQKKNDWKNLKQEVY
                     HSPDVISFAKAHDFYKGTDAAFSFSDVYNPITFDGSRFCDARVWAFFRKVNEGVRSNT
                     SFTQYACGQLVYAKTFADGSANTNNFCTNRLPLWVKPDELIDIQLVMATMRDHFEDTP
                     LDMRDGIGAGPWHCPYRWRPMTFEIDSIEYVHERAIATQQTGYSFVAQSRSWLDDVVG
                     GIYWFGVDDADGCVYAPMYASISEIPSSYAFGTGSMIEWNDNSAFWIFNLVTNWCYTR
                     YDLIHPEIEVYQKELENGYLARIADIDAAAIALLKSDPEAARKYLTEYSCTTGNKLVQ
                     DWRVFFQYLFMKYMDGNVKESDNHKLLDNGNSKGIPKKITYPGYGEDWLRLMMMNSGM
                     RYTMPVKK"
     gene            complement(12665..13873)
                     /locus_tag="PKY63_03705"
     CDS             complement(12665..13873)
                     /locus_tag="PKY63_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99752.1"
                     /translation="MLLKSIITQIAEQQQIAMRSAPNPFRREALSALHPLQSHAQIIA
                     GIRRCGKSTFMQHYLKINHRGAFFLNFEDPRMSYFDMNDWQRLDEVIADTGKKVLFFD
                     EIQYMPEWERFVRKKLDEKFQVFITGSNASQLHSELGTKLTGRHLDYTLFPFSFREFA
                     KYRKLKPSADSVLDYMETGGFPEFLISGKTEILSQLFSDILMRDVALRYGLRDLKSLQ
                     QLALFLISNTGNRITANKLKNTFNISSTVTMIEYLSHLESVFLFFFVPRFSYSLKVQN
                     QNAKKVYTIDTGLIKANTVSFSKDLGNLFENMVFLHLRRKHEHIFYYSEKQECDFVVV
                     EKNHPEFLIQACYELNADNLERETGGLFEAMSCFGLKKGTIVTLNQKDSFKQNGQIIN
                     AIPAHEFLSL"
     gene            complement(13999..14970)
                     /locus_tag="PKY63_03710"
     CDS             complement(13999..14970)
                     /locus_tag="PKY63_03710"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HPE99753.1"
                     /translation="MKKEDAQNSMNRLGQKGIPFLFVLDYALSECLVIPLSEIDPSEI
                     MYNLQGKSNENVVVEIPDKIEFSKTPVSFIAYQKRFDIVFRNIQHGNSYLTNLTQPTQ
                     VSTNLSLQQLYCVGNAKFKLYFKNQFAVFSPEPFVKISQGKIFSYPMKGTIDEDIDNA
                     ADILKNDFKEKAEHNTIVDLIRNDLSMVAKNVTVKRFQYIEKISTNYKTLLQMSSEIC
                     GDLPDDYLNHLGDILFKLLPAGSITGAPKEKTCEIIHEAEGYEREFFTGVFGVFDGNM
                     LESAVMIRFFENTPDGLIYKSGGGITCFSQAEKEYQELIDKVYIPRN"
     gene            15003..15572
                     /locus_tag="PKY63_03715"
     CDS             15003..15572
                     /locus_tag="PKY63_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPE99754.1"
                     /translation="MKILLIDNHDSFTFNLVHLLRSFNGIEVDVIPVDALQLEDVAPY
                     SKILFSPGPGLPSDRPLMREIILRYAPEKPILGVCLGHQAIACSFGAELGQVTPVYHG
                     VTAKVKILNRNCRLLNGLNDGFDAGLYHSWAVSETHFPSNLEVTAISEKGIIMALQHR
                     KYDIHGVQFHPESIMTPEGKNIISNWLSK"
     gene            complement(15613..16035)
                     /locus_tag="PKY63_03720"
     CDS             complement(15613..16035)
                     /locus_tag="PKY63_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE99755.1"
                     /translation="MDIHDCIRFANENPLCILATAEGKQPRVRAMDMWFADETGFYFQ
                     TSTIKEIPSQLMGNPKVEVCFIRNKTNPLLMLRIAGEAEIILDPAYKNKVMEDKPHLQ
                     AFVSRPESPKIVLFRITHGEAHFWSEEINLRPKDFIEF"
     gene            complement(16134..16799)
                     /locus_tag="PKY63_03725"
     CDS             complement(16134..16799)
                     /locus_tag="PKY63_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPE99756.1"
                     /translation="MTNIYPDTLQFLREVKENNNREWFAPQKHRYVAARENIGKWLGE
                     LIAMMKQSDDRILTNPPRGVGRIYRDIRFSPDKTPYRTYLGAMVFRAPEDRNCEFYIH
                     FEPGNIFAGGGIYMPDSAQLKLIRDDMAYSTKELEKIVKKPDFKKYFGEVSGEKLQRA
                     PKGFSPEHPAIEWLRFKQFLVLRSFNDRQVLQKNFQEEVYKTFQAARPFFDHIDRALN
                     FKE"
     gene            complement(16999..18876)
                     /locus_tag="PKY63_03730"
     CDS             complement(16999..18876)
                     /locus_tag="PKY63_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPE99757.1"
                     /translation="MNYLSIENLTKSYGEKLLFQNISFGIDEGQKVALIAGNGTGKST
                     LLNIIMGLDIPDSGTVTMRNDIRVAYLPQAPQFDPELSILDVVLTTDNLYVKATREYE
                     LAVELVNHDPSVFNQRLLSEATTQMDALAAWDYDSRVKEVLSRLRIGDLNRKMGTLSG
                     GQVRKAALARVLIDETNFVILDEPTNHLDIDMIEWLEEFLQKQKMALLLVTHDRYFLD
                     NVCDEIFELDNCSMYQYDGGYAYFVEKKAEREASERLETEKARNIYRTELEWMRRMPK
                     ARTHKSKSRIESFYELEIKAKRCLDDVRPEFQVKERRMGKNILEINNIHKSYEPVGAV
                     IDDFSHIFKRGERVGVVGPNGSGKTTLFKIIMGEERPDLGSLKTGQTIVFGYFSQMNP
                     DIKEGKRVIEVVKDVAEEIVMGNAPSVSASQFLHYFGFDHNTQYNYYSNLSGGEKRRL
                     HLCLTLIKNPNFLILDEPTNDLDIDTLNVLEEFLIGYPGCLLVATHDRAFLDKIADHI
                     FVFEGNGKVKDCYSNYSAYRLQKLKEENLEKVAAKPEEKPIKPKAQSNKPTFRQKQEY
                     EKLTTEIAALEKEKAALLEFMNSGNDDPKKLTEASTKYSDITNRLEELELRWLELSEL
                     F"
     assembly_gap    19027..19126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19181..19280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19688..20296
                     /locus_tag="PKY63_03735"
     CDS             19688..20296
                     /locus_tag="PKY63_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPE99758.1"
                     /translation="MNFKSFLFASFFLFASMLQAQTDASGSSQLKMGLHGGLNLSKYQ
                     TELDSTDASARIGWQGGIMLRYGGNFFIEGNLNLGQSSANMMTTDSIGTIETTVYRTF
                     MALPVMAGTKIFSSDDGSSSIRLMAGIELMSVLKTDVSDSLLYIEADDFKPYSLAAVG
                     GIGMDLWFLRFDLALRYGLTPMLSTDPDSKIVMGTFNIGVIF"
     gene            20309..20884
                     /locus_tag="PKY63_03740"
     CDS             20309..20884
                     /locus_tag="PKY63_03740"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPE99759.1"
                     /translation="MKFELPPLPWAADALEPIISAKTIEFHYGKHHQAYVNNLNNLLP
                     GSPFENAPLEQIVVEAEGGIFNNAAQVWNHTFYWNCLTPKGGGQPTGDLMDAIVRDFG
                     SFEEFQKQFTQAAATLFGSGWAWLSADEDGKLIISQESNAGNPMRKGLEPLLTCDVWE
                     HAYYLDKQNQRPDYIADFWKLVDWEAVSDRY"
     gene            21069..21287
                     /locus_tag="PKY63_03745"
     CDS             21069..21287
                     /locus_tag="PKY63_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPE99760.1"
                     /translation="MKQETFSLRGQEYIQLDQLMKLLGWIESGAMAHKVIDQGLVKVD
                     GETELRRRRKLREGMVVEFEKIRIKIKR"
     gene            21293..22111
                     /locus_tag="PKY63_03750"
     CDS             21293..22111
                     /locus_tag="PKY63_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPE99761.1"
                     /translation="MKLFYLLPFLLIFSCAYGQNTKALKLFNKGKIAFESKDYLKAES
                     FLQKSIKKSASDLAYYYLGMTQLILDDTCSACNCLLNAKIRGNSDATILFSNECVQCD
                     TINYRGGSYFCVVTKWTCTNELEAEFFKRSKKIGSDTMVFLENDSAINSFLFYSSEFE
                     IEKYLDKTTPGIVPEVPEFPGGESGLMDFLRDNIKYPAYAREKNIQGTVFVRFVIQKD
                     GKVSLVRVLKGAGGGLDEEAIRVIKAMPNWKPGIKDGKPVNIIYNIPIRFILAG"
     gene            complement(22254..23246)
                     /locus_tag="PKY63_03755"
     CDS             complement(22254..23246)
                     /locus_tag="PKY63_03755"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPE99762.1"
                     /translation="MKRIFLVLAVGVVATLITSCFKKTESGLPPSNGTVAEVLVVMPN
                     QQWETSVGAAVKSMLMAEQEGLNQSEPLFEPLQIQHQDYKGLFERNRKTIRFFVSDTV
                     KKNQLVARRDVFSSPQLIIDLHAKSDSEAIRLLNERAGSVVEMLREVERERLILAFSS
                     TQNVALCEKIEKQFGFSLVIPESYYMAKSNDGFAWLRLETAKYSQAILIYSRAFTDSA
                     QFNPSQIIAYRNMIVKQNIPGELPGSYMSTDTIAPPVMRNVPFADTRAIEARGLWKTV
                     GDFMGGPFLSYTFHDKKANKVITLEGYVYYPSMDKRDLLMQLEAILHSYEVKTK"
     gene            complement(23252..24748)
                     /locus_tag="PKY63_03760"
     CDS             complement(23252..24748)
                     /locus_tag="PKY63_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPE99763.1"
                     /translation="MKYLIVIFLFFLPVFAVMSQPTDTVINVENTEIAKIFDEMTREK
                     FYNSDNFFPEKNKYNFPANYVPEFNDSTLAARVKAMDEASPFEYRYNPEVKKWIEFYI
                     SKRYFIGRLVGLSLLYYPVFEQAFDKYQVPLEMKHLAVVESALNPTAKSWAGAAGLWQ
                     FMYKTGLLYDLNVTSYVDDRYDPLKATEAAAHHMQDLYAIYHDWALVLAAYNAGPGNI
                     NRAIKRAGGETNYWKIKHLLPKETQAYVPAFIAASYVITYYAEHNINIQRPQFMDWEL
                     DTVSVTETMSFNFFTSFVGIDESEMVFLNPQYIKKIIPGTKNNPCAIRLPQKYVLAFV
                     EKEPLMYDSIHKVAKADSALIATNTTGTVKTNPVNNTTVNNNTTVNTSGSKNYHTVKS
                     GETLGTIASMYGTSSSSIQAWNNLSSTVIYPGQKLLVYGKTANSSNHISNTSNGSLKT
                     VYYTVKKGDTLWKIANANGVSVDAIRKVNNLKSDNLWVGQKLKIHKNS"
     gene            25000..25656
                     /locus_tag="PKY63_03765"
     CDS             25000..25656
                     /locus_tag="PKY63_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99764.1"
                     /translation="MSDLLQEYKDYYRVRADRYAENNNYKNSYEAEKNLSEAIQGCNI
                     LDEFKDRLGNLNHLCAIALTKDKNLMEKAICEELNEPIRGNIPERILAKADQYTEVFD
                     LINMVNEENTRGMREISLDEANRIFHHCWMLLDRIETYSEVLVPPAYQADMKSNAEYY
                     RKRILEIIKDTEQQMQMLDPAWKHNPDIIKEYRHRRLLPYRDEHIDEQLSKYKTIANI
                     "
     gene            25668..27101
                     /locus_tag="PKY63_03770"
     CDS             25668..27101
                     /locus_tag="PKY63_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99765.1"
                     /translation="MPLDPMMTDALLGAFKNMVQECRDKNMSGEHFDRMCELYNHLEE
                     LTQTHDDVIAFNMQVMKEDIYVKISDCYSRLLSAQAQEEQKNIGYDDSALLKQNLNAL
                     RDAIRRIEDEKERVLAEARNYDPHKAMNDTLDFAYRNEGKKGGLVKGVVDNSGGLDLI
                     KSESKKMLDSELKERPNAYNSDVEIDTLFKTELYTKPIEDLIKLGEEPGMTYPRYLRI
                     QIENGLDKAMEGSAATREGLVFLLGMTEALPASPHHIECEKQKLELFDRLASESKIHV
                     PNSDELGFGIRRINHHIEPLKIEWTEIKSRWEDLIYDLYIWSLSYCPFAPQIQPWSLS
                     ENPREAVIRTQKTQPGLLKQHEKLFQKYFGLEFFDIFKHPTFEWEVRYKYLSSSQEFL
                     EFLIEKIYPVCIPLQDMPSELIPLRSAMFKDNKEMNPDLDKTILPMVEYYNSVFGQGR
                     WQSKYQMPEKSKSTAAVWNWQMFKYKN"
     gene            complement(27356..27919)
                     /locus_tag="PKY63_03775"
     CDS             complement(27356..27919)
                     /locus_tag="PKY63_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPE99766.1"
                     /translation="MNCIWLIILSIGLCFDTLAISISIGINDRRIRFKQALRIAFIMA
                     VFQGVMPALGWLGGVSLEQFIAPVDHWVALVLLTIVGGKMILESFGNDEKDPMDINNM
                     KLIFTLAFATSIDAFAVGIPLAFTNINIWIAAPVIGLFTGVTAMIGMFIGKKAAGFLG
                     PKIQIIGGLILIGIGLKIFLEHIEVLK"
     gene            27994..29856
                     /locus_tag="PKY63_03780"
     CDS             27994..29856
                     /locus_tag="PKY63_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPE99767.1"
                     /translation="MKKLFFVFFALSAFGLTAQNFSITNYTTSNSPIGSNSITALKGD
                     SNGNMWIGSSDNGLTVLSSSTGTILTTTDGLVGNNVKDIDIDGLGNVWIATTAGLSKY
                     NGSTFTNYTTAQGLPSNDLRCVFVDASNNVWIGTSAGVCKYDGSFTTYTTTDGLIHNT
                     VLAITQDLSGNMWFGTAEGVNKLAGTIWTDHTFLNGVNANGDQVQSATTDADGNIWVG
                     SLPSMGLGGGLYKWDGTSWTTYTTTNGLAHNDVRGIASDPMSRIWIATNGNGTSHFRN
                     ATFTTYGTSTGIISTSQTCSEVDYNGFVWIGTNAGISRISTLVIQTSITTNAHCGNDD
                     GSLTINVGTINGPVYFTYDNGVTITTNNTLNNLTAGVYQVQLTDSSIFASMAIEIFSS
                     TQAMDAITETTYNLCDGDSMLPGVDPAFTNCLWDPAAVVSDANIPNPLISPTTNMWVS
                     LTYTDTNGCVGEDSIQFFIMPVPTVFVNITNDSVFTSPLIFNNYQWFLYGDTIPGATG
                     QSYTATQPGVYMLCANNTGQCVGCSDPINFQNAGFDENNDWAKIFANDGILHWMLPSE
                     GCMISIYTPDGRLLQQFIASDKNGTLTLKNNVSGIILIQVENGDRSAWIKAFAK"
     gene            complement(29923..32328)
                     /locus_tag="PKY63_03785"
     CDS             complement(29923..32328)
                     /locus_tag="PKY63_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99768.1"
                     /translation="MKKIVLFFAGIVLFSCLQAQVVTVKDRQTGSPLDLVTLTSAQPH
                     AFAVTNPEGQADIRAFKGVEKIEIRILGYKTETLSYAQIEASGFIVMMTQTGINIDGV
                     VVSATRWNQVSSGVPSSIISISSKSIALQNPQTAADLLGSSGKVFIQKSQQGGGSPMI
                     RGFATNRLLYTVDGVRMNTAIFRAGNIQNVISLDPFAVENTEVFFGPGSVIYGSDAIG
                     GVMSFQTLTPKLSMTDQPYITGKAVTRYSSANNEKTGHFDVNVGWKKWAMLTSISTFD
                     YGDLKMGSNGPDEYLQPYYVQRQDSVDRIMTNNDPRVQTPTGFTQMNIMQKIRFSPNE
                     KWDVQYGFHYSETSEYSRYDRLIRYKNGLPRYAEWFYGPQKWMMNNLSVAHNKKTRLF
                     DQMSLRLAQQYFEESRISRDINKADRETRIEKVDAYSVNLDFNKSIGKKYTLYYGAEY
                     VWNKVTSIGVDENIVTNSVQDGPTRYPQSDWSSYGVYLTYQHKVSEKFMVQGGLRYSA
                     YDLEAVFDTTFYPFPYTRAELSKGALTGSLGFVLRPFDKWVVTANVATAFRAPNVDDM
                     GKVFDSEPGSVIVPNPGLEAEYAYNADIGVSKVFGEFLKVYTSGYYTLLQNAMVRRDF
                     QLNGLDSIMYDGELSQVQAIQNAAVANVYGVQAGIEVKLPKGFGFETDYNFQVGVEEL
                     DDGTTSPSRHAAPRFGVTRLSYHYGKLDLQFYANYCAEVSFENMPEDEKGKTEIYAQD
                     ENGNPYSPAWYTLNFKAMYQMNNTFTVSAGLENISDQRYRPYSSGIVAPGRNFILSLR
                     ANF"
     gene            complement(32403..32933)
                     /locus_tag="PKY63_03790"
     CDS             complement(32403..32933)
                     /locus_tag="PKY63_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99769.1"
                     /translation="MKKVFALSVLVVFLLSVAGGQFLLFKAYRLHVRSLAKAALREMA
                     ASEADVIAFSKSDFSHGLPFFRVPDKDDEIILHGEFYDVKDVEISSDSVFLFAYRDSN
                     EKFLIAQFEKSARDNDQSPLAVLLHQFTTIFTFIPATIEKFSVVNNSSDIIFINPGFI
                     YNCEKSDVISPPPRLI"
     gene            complement(33021..34400)
                     /gene="nhaD"
                     /locus_tag="PKY63_03795"
     CDS             complement(33021..34400)
                     /gene="nhaD"
                     /locus_tag="PKY63_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HPE99770.1"
                     /translation="MFLLMVIVFVLGYLAIALEHPLKIDKAASALLIGSLTWVVYISG
                     AFDIFTSGVSTAWNEYFAANPEANTIEGMQHFIVHNQIIEHLGEISEILFFLLGAMTI
                     VEITDQHEGFKVITDRIKTTHKVKLLWIISFISFFLSAMLDNLTTTIVMVALLRKLID
                     DKHTRWFYASMVVLAANAGGAWSPIGDVTTIMLWIGGQVTTVNIIVGVFIPSLVCMVV
                     PLLLLSMTMKGNVTRPKIDAEKKSKTTSFERNLMLGLGVGGLLFVPVFKTITHLPPYM
                     GMLLSLAIIWMITEILHFKKPKEQKLSVTSILTKVDVPTVLFFLGILSAVAALQSAGQ
                     LSVLAQALDDSLGDVYLIGGAIGVLSAVVDNVPLVAGAMGMYPIADPGATGYLANFVQ
                     DGLFWEYLAYTAGTGGSMLIIGSAAGVAAMGLEKIDFIWYLKHISWLALIGYLAGIGF
                     YVLEHMLMG"
     gene            complement(34527..36953)
                     /locus_tag="PKY63_03800"
     CDS             complement(34527..36953)
                     /locus_tag="PKY63_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPE99771.1"
                     /translation="MSSVFFKYHLIAFFFAALLLAGCSTTRRNPGKILLTDTKLVIDN
                     KALDYYDLDGLIRQHENRQFLGMRLHTGVYNLGALMKDRTKKKEADIRKKIEKKKKRN
                     PNKYINEQKELEKAKRGLRSWLMNTVGEEPVFLDSGLVKQSVSQMELYCKKHGFFNAE
                     VSDTMIFNKKNNKAKVEYRIKAGVPYIFNKIEFVSGDADINSYLISGKANSLLQQGNI
                     YNEDVLESERMRINDDLKNLGYFAFSKSYIRYEIDSTIGNRQLNVKIIINRPFQQVNG
                     VTEFYNHRKYKVRKLVVLPDFSVTSEGVVKYDTLHYVKHLRNRKQADTLCFLYQEAMR
                     VKPKVIARKIFIRTDHYFNLSNANKTQAELSSLNIFKYINIRFVPDTAGVQPDQPIPL
                     DAFVELSLSPVQAVNVEIEGTNSSGNLGTAGNLVYKNRNLFHGAEQFNFRFKGGLELQ
                     KTVFEEKNDVISGLPFNSAEISTEAGLTTPVNNSWFTQSSHPMMKYSTGFSYQLRPDY
                     ERYISHLKATLEWKESATTVMQFYLPINLVRILPDSLFAERISQFSRTIRYSYEDHFI
                     PGCGFSMIKSNQALRKNRSYFYWKLLIEQAGVFMLWPGNSFNSARQGEIYRVLGINYS
                     QFAKVDIDFRYYKPWLNQTTFVSRFFFGFGIPFGNSMLLPFEKSYSASGSNDIRAWKF
                     RSLGPGTYTDSTFFDRTGDISMVFNFEYRFPIISWFNGALFVDAGNVWLKNASEEFPG
                     GEFPGLDFYKQFGVGTGLGLRLDFDFFIFRIDASFPLRDPALPDGEMWVGFDKPLKST
                     TLSFGIGYPF"
     gene            complement(36946..38190)
                     /locus_tag="PKY63_03805"
     CDS             complement(36946..38190)
                     /locus_tag="PKY63_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE99772.1"
                     /translation="MNLKIIGESFNIALRSVRSHLLRTILTVLIIAFGIMALISILTA
                     IESIKFSLSENFTRMGSNTFTITDVQMRGSRGRSSNDRVEFEDITWDQATTFKKRYQF
                     PATVSVFTHASGSSTVKYNLEKSNPNVGVLGIDENYMITSGQEIEKGRSFSRHEVEMG
                     RNVTVIGSEVEGVLFPTGENPLGKQISIGPVRFVVVGVMKTKGSGFGFSGDNSCLVPV
                     TTVRRYFSKSDVSYRINVMPIDTKLLEAATGEATGVFRTIRKLKVGQADNFDVRQSDS
                     MVNMLLENIRYVTLAATLIGFITLIGASIGLMNIMLVSVSERTREIGVRKAMGATRVA
                     IRNQFLIESIVVGQMGGLIGILLGVLAGNVISIYLDGIFIVPWGWVILGFTLTLIVGI
                     LSGLIPANRAARLDPIESLRYE"
BASE COUNT        11334 a         8409 c         7665 g        10691 t
ORIGIN      
        1 cctcccattt gcagcggtgc atcagtgaat tcgagcatat ccttgtattg ccgcagatga
       61 ttcctgattt ctttgcgcaa aattccaaag ccctttccgt gcaaaacaga aaacgatctg
      121 acgccggtaa gtaatgcatc atcaagaaat ttatcaagct ccattatggc atcaatagcg
      181 ctcatgccgc ggtaatcgag ttgataaact gtttcctttt tttcagaaat aagagttact
      241 tttttctttg gctgatgctg tttctgcttt tgggtagcaa caaccgaaag ccgcgccgaa
      301 tcaacggtta atgaagctag ttcaaactga accctgactt ttttattctt gatttctatt
      361 accgttcctg tttctattcc atcatcaagg cgcactgaat cacctactgc aagcggtttc
      421 ttaggtgcat catctgtatg aattgtcttt ttcaaaggca atggttcaat cggtttttct
      481 ttcgtagttt tcagcgatga tgccttcttt tcaaactccg tacgaataac tctcacctta
      541 tcttttgcgg cctgctcctg ctttatgtcg cgaattgttt tttcaatcag cgcattcgcc
      601 tctgaaagaa tatttttcgc ttcgctctcc gctttcgaca taatgcggtc gcgtttctca
      661 acaatgcttt tgttcagctt attgtatttt tcaatcaatt cgctcacgaa atcctccgca
      721 acttcaatct gtctgcgccg gtcttccagt ttcagttttt cattgtccag atcctgaatc
      781 atcttctcca tctcaatgcg cgccgtgcct attttcgaag aagcttcttc aattaatgct
      841 tgcggaaagc cgatggatgc tgcaatttcg agcgcaaacg aacttccggg cttgccggtt
      901 tgcagcttat acatgggttt cagcaaatca ttgtcaaaca gcatcgcacc gttaataaca
      961 cctggatgat ttgttgcaaa cattttcaga ttaaaatagt gtgtggtaat aacgccatac
     1021 accccttttg cattcaatat ttcgagtacc gcttcggcaa tggcaccacc actttcaggt
     1081 tccgtaccgc ttccgaactc atcaatcaga atcatggtat tgctgttgcc ccgctccatg
     1141 aattttttca tattcagcaa atgcgatgaa tacgtgctta aatcattttc gatggactgc
     1201 tgatcaccga tatccgccag aattgcttca aaaggataaa gcatggttcc ttcagcagca
     1261 ggcaccagaa aaccgcactg gaacatgtat tgcagcaatc cggttgtttt catggccacc
     1321 gattttccac cggcattcgg accactgata atcattaatc tttgtgcagg cagcaattca
     1381 atatcgagcg gaacaacttt cttaccttgt ttgagcaacg attcttcaag caccggatga
     1441 cgtccctgca taatcacaat tgaggcttgc tgcggaatta cggggaccac tgcattttgc
     1501 tcaacagcca gctgcgcctt gccccgaata aaatccattt cacccaggaa atcaatgtga
     1561 gaatgtaatt ccggaaccga aggcctgatg cggtctgcaa gtgatgtcaa tatatgatgt
     1621 atttctcgct tctcttcaaa ttccaggtct tgtacttcgt tattcaattc aaaaatttca
     1681 ataggctcaa caaaaacagt ttgtccggtt gcagattcat caagcacaac gccttttatc
     1741 atgcgctttt tcgatgcatg caccggaatc accaaacggc ctccgcggat gcttagattc
     1801 acttcctctt caacaacacc tttttccttc agacttttca gcattgcatg tatgttgcgc
     1861 tcaatctttt tgcgttgtcc ggtaattgac ttacggattt cggaaagccg ctccgaagct
     1921 gagctgcgta tgtctccgtt ttcgtccaga atttcattga tgcgtaccgc cagagaaaaa
     1981 tcaaacggat gatcatcaag tcgtgctttc agcaatggtg ttttatcatc ctgctgacga
     2041 aacaattgtt tcaggtcgtc acatttttca agtataaatg caatggcttg cagagttgga
     2101 atttcgaccg caataccttg tgttttcaga cggctgagct cttcgcgaaa atcttcgaaa
     2161 cccagagctg gaacgcctcc cagatattcg agaatatatt tcatctcagc cgtcagcaaa
     2221 agcgactgct gaattttatc ggtatcggaa gtgaaaaaaa caccggcagc cctctccagg
     2281 cctgtaggtg taaaacaaac agattgcagc ttttttctga tctccgaaaa gccaagcgat
     2341 gattcaacgg cgttgtaagt attcaattct ctctgaaatt tttgacgtgg caaaggtacg
     2401 gattattgta ttctgactga cctgcaacag gttaaatatg aagcttcaat ctgcattttc
     2461 ggaatactta cctgcatttc taaaagaaat gagagaaaaa aatcagcaga taaattaaca
     2521 gcaacatggc tgtaaaagct tttaccagta atttattttc gagttttttg gttttgccga
     2581 ataaaataat cagcgtcagc acaacaatga tatacacaac aacaataatc tgattagcgc
     2641 tatcgctcga ctcgtagcgc cttcccatca tttcacagga ttctacaaag ccgaaaaccg
     2701 gtgtgtaatg tattgttata acactatcgg tcattgtttc tccagccaag ccgggcatca
     2761 gagacaaatc gaccaggtcg ggcgaaaatt ttccgttttc atcacgccct acaggtcgct
     2821 gaacatcgcg ggttggcagc cagtgatcag caatcatcac gaaacaaaaa gcaagaataa
     2881 taaaattcac ccatttcggc cataaaatac tatgcatcgg catatcaaaa gcatcgtttt
     2941 tccttttgaa ttcctcgtat ttcatacgtg catactgcct ggccttttca cgcgcagcac
     3001 gcgccagatc atcggccgaa tatgcaggac tgcttgttcg tgaagtcggc ctgcagcgtt
     3061 cacctatcag aatttcatac gcctcggtaa cacgtataaa ctgatcggaa gcatccggag
     3121 cagggttttt atcgggatga aacttgagtg caagtgttcg gtacgccttc ttgatttcct
     3181 gaatggaagc gctttccgga accccaagaa tctgatgata catcgacacg atgttgataa
     3241 ttaggtaaac tgttcgcctt tttcaatcat aacatcgttg acatactgac ggatgttctg
     3301 ttcatcttct tttctaacga ccatcagaat gtgatcggac tcgacaacaa tataatcgtt
     3361 cagcccctgc aacaatacca ttttttcctg aggcatgtgg acaagacaat ttttcgaatc
     3421 gtacatcatc acatttttac cgacaatggc atttccgttc tgatcgcgct gccggttttc
     3481 gtaaagcgag ccccaggtgc caagatcgct ccatccgaaa tcggaagcaa caacatacac
     3541 attcgatgct ttttccatta ttgcgtagtc aatggaaatg tttttgcaaa gcatatatgt
     3601 ctgaaaaata aagtcagctt catcaacggt gcgatagatt ttatttccct ttgcaaacaa
     3661 ggcactcata tcaggaacat gtttctgcaa agcatcgatg atgcttttgg ttgtccagat
     3721 aaaaatacca ctgttccaga gaaaatcacc acttttaatg aattcctttg ccagctccag
     3781 ctgcggtttt tctgtaaagg tttttacttt tttaattctt gaatcaccct ccaccttttg
     3841 gccgtcgagg aactgaatgt acccgtatcc ggtatcagga cgtgttggca tgatacccag
     3901 cgtcatcagc cagttgtttt tctgcgatgc aacagtagct gatctgagat gttcaatgaa
     3961 tgcatcctcg tcaaaaatta tgtgatcgga tgggcaaaca atcattgttg catccggatc
     4021 gaaggagttt attctgtatg cagcataggc aatacatgga gccgtatttc gcctggatgg
     4081 ttcagacagt acattgcctg gatttattaa tggcagctgt tttttcacca gctcaacata
     4141 attttcattc gtcactacta gaatattctc agccggacaa attttcagca gtcggtcaaa
     4201 cgtaagctga atcaatgttt tgcccatacc aagcacgtcg ataaactgtt tgggcttcaa
     4261 tgtacgactc atgggccaga aacgtgttcc gagaccccct gccattatta cacaataagt
     4321 atttcccgcc attatttcca atctgttttt tgcaaaagta gttatttttt tcgttggttt
     4381 cagattgcaa tttcatcaat gatttgatgc ggtttcttca atattaaccg gtcattgcct
     4441 gtatacttgt aaatgatttt ttttcataac aactaaaggt taccaataaa ggcgatttgg
     4501 tatcgtttcg gtaacattga attaacattg gtttgatatg tttgccgaaa attaatcctg
     4561 tatgaataaa tcattagtaa tctttctgct ttttgccgca gcacttgcgc cagcaaatct
     4621 gatggcacag gataccataa agcctgcaaa atcgttttta aaaaacacag aaatctcagg
     4681 ccaatggttc atttccggca attacgagaa tcatgactca ttgtatcaga ttgagctcaa
     4741 gcgcggctat ttcaccattg aaagtcattt gacggagaat ctcacggcgc gctacacgca
     4801 ggatatcaca ctcgatacag aaggtgacga tgcaggaaat gtcgaaatgc gactgaaata
     4861 tttatacctg cagcagaaaa tacactttct tccgaaattt cagcaggggt cgttttcgat
     4921 aggaatggtg ccgcgtccat ggattgagtt tgagcaaaag atcaattctt accgtgtcat
     4981 gggccccatg tttcttgaaa aaacaaatgt gcttagctcc gctgatttcg gatttattta
     5041 tgaagggctg attggtggcg agattgacaa gcaatatcag aaagaagtca atgctgctta
     5101 tcccggaaaa tacggcagtt tttcatttgg tttgtacaac ggtggtggtt atcacgatgt
     5161 tgagcgcaac aacaataaaa catttgaagc acgtctgaca ctacgacccc tgcccgcttt
     5221 catgccggga ctgcaattcg gctacgccta tgccaatggc aaagcgaata ttgcaaatga
     5281 cgcagacttt aatatgaatg tgatttacct ttcatccgaa agcaaatacc atgttttcat
     5341 ggcacagtat tacagcggtg ttggcgatta tgcagggttg tatactgaca cattattcaa
     5401 ctcatacaaa aattcagggt acgctttgtt cggagaattc aaaattcctc agacaaaatt
     5461 ttctgttgta ggtcgctatg attctttcaa atctgaaaac gaattttctc tgacacgcat
     5521 caatacgggt atagcatatc gttttctcaa aaacaaactg gttcttggcg ccgaatactg
     5581 ggataaaaac ggcgatatca gacgcatgtt tgaacttgcg ctcgaaatac ggttttagct
     5641 tctatggatt tgccacgaat gcacaaagac actaaatatt aaatggcgtt ccaattccct
     5701 ttctgagaca aagattcggc ttatataata taactgggat atactggaaa accattgccg
     5761 cacttccact ttttcgtcct cgatgtttct atggatttgc cacaaaggca cacagacact
     5821 aaatattttc tccgcgaatc cgccagcagg cagacatgtt cacgcatatt aacgcaatag
     5881 aatttcaatt taacagcaag tcttataaac gccaatagaa tcaatcttca cctgtctgca
     5941 atttcaactg caacaggcag tcaggcaggc cagcggtgaa ccttgattcg gtgttgaagc
     6001 aaatgatgcc tcagaggcac aaaggcttta aatatttttt agtgacttag tgtccttgtg
     6061 gcaataattt ctattaccgc atgccttatg cgccatacat cacattcact tccggaatca
     6121 agggcatgta atttagccgg cgcagcaact gaatcagcgc aacaacatct ttctgacaat
     6181 actgcataat gcggatcaga tcgtcttctt cgtaatacac gcgggcaaca tcggcaccat
     6241 tgatatcatc cttaggtgtt ggaatatcga atagttccgt gagcagatca agcgaagtga
     6301 aatttttgta atcaccgaat ttccacaatt ccatggtgtc gatatgcgca atttcccagg
     6361 gcttctttcc ctgaatattc agcatatccg gtatgcggat tccgttgatc atcatacggc
     6421 ggcaaatcca cgggaagtca aattcctttc cgttgtgtgc acataaaagc cgcaccccgc
     6481 gcgatggaag gtcgcgcaga taatcggcaa actcattcag taattctttt tcatcatgtc
     6541 cgaaaaagct tttgatatgc gcctttccgc cagctatgac acccgtggaa atgcagatga
     6601 tttttccgaa ttccgcaaaa ataccggcct tttcatacag agtctcaggc gtatcgtttt
     6661 cagcccgctt catgctttcg gcttttttgt cccacaggtg cttcatgcgg tcgctaagct
     6721 tattgtatga gggcatggcg ggaaccgttt ctatgtcaag aaacagaata tgagaaaaat
     6781 attcttccat aacagggtgt tttaaatttt ttcgtgctaa atttataaac aaaatccgac
     6841 aatgcgttac aaatggccta ttttatttct gcttaccctg atttttgttt cttcatgccg
     6901 cgaagaaaca tatgagcccc ctttcattca aatgttctca ccgtcagaaa acggcatggc
     6961 tcaaaccgga gatacccttc aggtaaaagg atccatttct tcagccattc ccattacata
     7021 catcaaaatt gttttactga catctaccta tatccaatca tgcccggcca caaccattta
     7081 tccaaacacc acaaactata atctgaacac agaatatatc atttcaaaca acaacctgtt
     7141 atcgggcaca cactacctgt ttattgaagc gggaaatgaa tatgcttctg gcaaccgttt
     7201 tatcaagctg acaataagcg gaattcccaa agagagcaaa gccctttttg cattttgtca
     7261 tgaaacagcc aatgatcatg taaaaatctt caaagtagat agtctgttga attacacatt
     7321 gtttaaacag ctccctgatg actttcagga aggggccgtc aacagccagg atcaactgat
     7381 ttattccatg ggtttatacg aaggaaatct gtattccctt gatgcatcag atggcaacat
     7441 ccttcatcag gtcaatgcaa ttattaatcc gccatttcct tattttgaat cactatactg
     7501 gggtaataat ctgctggttg ttggttacta cgacggacgc atagaaggat attttggaaa
     7561 cgggaacctg aaatttgtct attccatcga aaatttcaga ctgcacaaag tatcctacga
     7621 cggattgtat ctgctttcag tgctcgaata ctatacagga accgaatact gtgcaggagc
     7681 catttatgaa ttttccggac tattaaagga tatgattttt gtgaattatg aaatcgtgaa
     7741 tttttaccgt gcttcaggaa acgacgtagt gcttttctgc aatgatgctt tacaaccaag
     7801 cgtcaaagca ttcaacacct atacaatggt aataacacac atcaaaaatc ttcctgcagg
     7861 gaccattcaa agcgttacac aaatagacga tgacaaattt ctgatttgcc acagtaatgg
     7921 ttttctgctt tactcttttg ataataactc atacgctgaa acaggcagtg gaagcacatc
     7981 cggagaaatt gtgtatgatg aaccagataa catcatctat ctttccgaag ggaaaaatat
     8041 ttctgcatat tcttatccaa cactacaaca tatcggaacc gtaatactat ctgacagcat
     8101 caaggatctc gaaattcttt acaaccgatg aaaacaaaaa aactcattat taccgaggat
     8161 ggctcgcaca cattgtttat accggagcta aacgaacatt atcattccat ccatggcgct
     8221 gtggccgaaa gtaggcatgt tttcatcgag gccggactga aaccgaaaat aattgagaat
     8281 aaaaaactca atgtactcga agtcggcctg ggtacagggc tgaacgctgc actggcttta
     8341 gtggccgccg aagaaaatca gatacaactc aactattttg cagtagagcc ttatcccatc
     8401 agcaccgaag atatgatgca gctgaattat tcgaaggtat cggaaagcac acgcatgaat
     8461 gaagtgattc cggccattca cgcggcatcc agagataacg aagtacacat tggtccgtat
     8521 tttatttttc attatttcga aaaacggata caggaaattg aatttgaaaa taattttttc
     8581 gacctgattt ttttcgatgc cttctctccc ggcgtccagc ctgagatgtg gacgatggat
     8641 gtttttcaga aaatcgcggc ggccatgaaa cccggcgctg tgctggtaac ctatgtcgcc
     8701 aaaggcgaag tgcgccgcac catgaaagcc tgcggcctga gcatcgaaaa actcccgggc
     8761 tttggcggaa aacgcgagat gacacgagct actaaaaatg ttttttgaca attcaacatg
     8821 tattgaaata aaaaagccgg aacttttcag aaccggctaa ttaaatattg aatcattcta
     8881 ttcgacgaca aacgttgcac gtttaatatc atcaccagta agtgttatgt aataaacacc
     8941 ttctggcaga tcagacacat tcagggattg gttacccggc tgcaaaaccc ttgaaattac
     9001 aagacttccc gcagctgtcc aaaggttcat cacacaattc tcttctacgt taatgctgat
     9061 aaaatcaata gccgggtttg ggaaaaccag aaactcattt ttatcaaaat cagttaaccc
     9121 agcagtcgat acaaattcta ccgtaacaac agcattttca attaaaccat caatagaaaa
     9181 attgtttgta gtatttccac tgataaaagc agaattataa atccattctt tcaccacata
     9241 acctgaatca ggaacagcaa tgaattcaac agaatcacct tcggtaataa aatcacctga
     9301 agaaatagcc aaaccgtttg cacttgctga aagtgaacca tgaccattta aaacactaaa
     9361 atcaacacta taagttgata tggtgtataa aggaatagcc atgccgcaaa cttcggcgtt
     9421 gttaaaacct ccaagacagg ttgatactgt tcccgtcaat gtatcgacaa catacagacc
     9481 accagcacct gatcctatat atgcagccag atacaacgta ttgttgttgt aatcgtattc
     9541 catatcctgt gcataactca atgccaaacc aattcctgag ccaatagcgg tagcagctcc
     9601 tgtcgtttta tccattgaat aaagaatgtc atcattcata ttcacagagt ataattttcc
     9661 cgaaggagaa caagcgagat taataaaagt agaagatgca acattggctt cgccgattaa
     9721 tgttacagtt ccggttaatg tatcgatact atacaaacct gtcgaagaaa caccatataa
     9781 caaatccgaa acaggatcat aagcaaggcc attcataacc acgcccatgg agccaattgt
     9841 ggttatagct ccagtagctg tgtcaatgtt gaccaatgtc atattggaat aatctgcacc
     9901 gtaccaggtg tcatttcccc aggtagcggc ataaatttcc gtatttgccg aatcaatcag
     9961 ataaatacta tccggattac tcagataaaa atacgaaggc cctgttggca cattgctgta
    10021 agcaccaaac ggaaagcaaa atactttttc gttgggtcct tttgtttttg gatcggcttt
    10081 agccggaaaa aatttctttt cattgtttac ggacttttca gcagtgctta cttgcgaaaa
    10141 cagcattgtt gaggtaaaaa agaaaagaat aaaagcagaa atcagtgttt tcattttgtt
    10201 aatttagttt tgcaaatata ctaatatata cattaactga aaaaatttta aaacaaaaag
    10261 caacctttta ccgctcaaat tgccaaaaac aaatccatgc ctcattacaa tattcattat
    10321 ttataagctt cctgacaact aaaataaaag aaaacaacaa acttttgtaa agagcgcaaa
    10381 agctcgccgc tcactattgg agagagtgtt ctgtctgcgt tagcagctga ccacaacgcc
    10441 tttttacatt taattcgttc gcttagcggg aagcgcgttt tacaacggtt gatttttgtt
    10501 ttcaaaggag cttcgcgtgg gctttgtgcc tggggctaac gcccatggct atttttgttc
    10561 ggctcctcca gagccgcttc aaaggaattc tggattatat ttttgtagta aacgttttat
    10621 ggccgcggcg gatggccaaa gcgcctttat gaactttaca aacttcataa ctttctactt
    10681 cttcacaggc attgtgtatc tcatgccgct gttcatcatc atcaggcgga gccagtcttc
    10741 gccatagcca gggtaggtga tttttttcgg aataccttta ctgtttccat tatccaacag
    10801 cttgtgatta tctgattcct tcacattgcc atccatatat ttcataaaca gatattgaaa
    10861 aaacacacgc cagtcctgca ctaatttgtt gccggtggtg cagctgtatt cggttaaata
    10921 tttccgtgca gcttcgggat cgctcttcaa cagcgcgatt gcagcagcat cgatgtcggc
    10981 gattctggca agatatccgt tttcgagttc tttctgatag acttcaatct caggatgaat
    11041 caggtcgtag cgcgtgtaac accagttggt gacgagatta aaaatccaga atgcgctgtt
    11101 gtcgttccac tcaatcatag atccggtgcc gaaagcgtac gaggaaggaa tctcggaaat
    11161 actggcatac ataggtgcat acacacatcc atcggcatca tcgacaccga accaataaat
    11221 tccaccgaca acatcatcaa gccaggagcg gctctgggca acaaaactgt aaccagtttg
    11281 ctgcgttgca atcgcacgtt cgtgcacata ttcaatgctg tcgatttcaa aagtcatagg
    11341 acgccagcga tagggacaat gccaggggcc ggcaccaatt ccatcgcgca tgtcgagggg
    11401 agtgtcttcg aaatgatcgc gcatggtggc catcaccaat tgtatatcaa tcaactcatc
    11461 gggcttgacc caaagcggaa gacggttggt gcagaaatta tttgtatttg cagatccatc
    11521 tgcaaaagtt ttagcatata cgagttgacc acaagcatat tgtgtaaacg atgtgttgct
    11581 tcgaacacct tcattcacct tgcgaaagaa agcccacaca cgtgcatcgc agaaacgcga
    11641 accatcaaaa gtgatcggat tatacacatc cgaaaagctg aaagccgcat ctgttccctt
    11701 atagaagtca tgagcctttg caaacgagat tacatcgggg ctgtgataga cttcctgttt
    11761 cagatttttc cagtcgtttt tcttttgaaa aggaaaagta gtgatacgag cctgattggc
    11821 gtgtgccgac acatacccgt cgggaatgcg catggcgacc cacacggcgc ctttttcgta
    11881 ggtacctttt ccgataattt ccatgatcca tgcttcgttt ttatcgctga tcgaaaacga
    11941 ttctccgcta ctggcatacc catatttttc aacaagttcg gcaatgatca taatggcttc
    12001 gcgtgctgtt ttggcgcgtt gcaacgcaag ttttatcagc gaaccataat caggtaaggc
    12061 atcgggttgc gactgcaatt gttcaagtcc gccgaaggtt gtttcgccta tggccacctg
    12121 atgctcattc atgagtccga ctacgttgta ggtatgtgct acttcgggaa tttgtccgag
    12181 cagttttccg gtctcataat gaaagaggtc gaccatatcg cccggcttgt gatcagccgc
    12241 cggataatga aataaatatc cgtagcgccg gtgcgaatcg gctgcatagg taatcatcac
    12301 cgaaccgtct ttcgatgcgc ctttggtaat gatcagattg gtgcaggcaa aggccggaac
    12361 gacgctgaga aacagcagaa gaaatgtaat gcgaagcata ggcatgattt aatgcgctaa
    12421 aataagaaat ttctgcgaag gacgtcgctg tgcgaagttg ccccgacatt ccatcgttgc
    12481 ggcgggtata acccctcgaa gagtttcaaa ctcttcgagg ggtgccggac tacgggaaca
    12541 tggaaccgtc ggggcaaggc aaaaacaacg gttccatgtc cgccatatcg ccagactcag
    12601 cgccaggctg gcggacgatg gaatgttgtt tttgtgaacg acttcgcata gcggagttac
    12661 agccttacag cgacagaaat tcatgcgcag gaattgcgtt gatgatttgt ccgttttgtt
    12721 taaagctgtc tttttgattt agtgtaacga tcgtaccttt ttttaaacca aaacaggaca
    12781 ttgcttcaaa caggccacct gtttcgcgtt ccagattatc ggcattaagc tcataacaag
    12841 cctgaatcag aaattcagga tgattttttt cgaccacaac gaagtcacat tcctgttttt
    12901 cggaataata aaaaatatgc tcatgttttc gccgcagatg cagaaacacc atgttttcga
    12961 aaagattgcc caggtcttta ctgaatgaaa ctgtattagc ctttataagt cctgtatcaa
    13021 tcgtgtatac tttttttgca ttctgattct gtactttcaa tgaataactg aagcgaggaa
    13081 caaaaaagaa aaggaaaaca gattccagat gagacaggta ctcaatcatt gttaccgtag
    13141 aacttatgtt gaatgtgttt ttcagtttgt ttgctgtaat tctgtttccg gtgttggaga
    13201 tcaggaacag ggccagttgc tggagtgatt tcaggtctcg taaaccgtag cgcaatgcca
    13261 catctcgcat cagaatgtca ctgaaaagct gagaaagaat ttctgttttc cctgatataa
    13321 gaaactcggg aaagccaccg gtttccatgt aatccaatac actatccgct gatggtttca
    13381 gttttcgata tttcgcaaat tcacggaaag aaaatggaaa taaggtgtag tcaaggtggc
    13441 gcccggttaa ttttgttccc agctcggaat ggagctgtga cgcattggat ccggtaataa
    13501 atacctgaaa cttttcatcg agtttttttc gtacaaacct ttcccattca ggcatgtatt
    13561 ggatttcgtc aaagaaaaga acttttttac ctgtgtcagc aataacctcg tcaagtcgtt
    13621 gccaatcatt catgtcgaaa tacgacatgc ggggatcttc gaaattaaga aaaaacgctc
    13681 cgcgatggtt aattttcaga taatgctgca taaaggtgct tttcccgcat cgtctgattc
    13741 cggctataat ctgtgcatga ctttgcaggg gatgaagcgc agaaagagcc tcacgccgga
    13801 agggatttgg agctgaacgc attgcaattt gttgctgttc tgctatctgg gtaataattg
    13861 attttaacaa catacttaat gtgtttattg caaatgtaca atattataca ttacaaatga
    13921 acaaaaggtt acattagtaa tgaataataa ttgcgaagga cgtcgctatg cgaagtatat
    13981 acttcgcata gcggaaactc agttgcgcgg aatataaact ttatcaataa gctcctgata
    14041 ctctttctca gcctggctga agcaggtgat tcctcccccg cttttataaa tcaaaccatc
    14101 gggtgtgttt tcgaaaaagc ggatcatgac agcactttca agcatgttac catcgaaaac
    14161 gccaaagact ccggtgaaaa attcccgctc gtatccttca gcttcgtgaa tgatttcaca
    14221 ggttttttct ttcggtgctc cggtgatgct gcccgccgga agcagcttaa aaagtatatc
    14281 tccaaggtga ttcagataat cgtcgggcaa atctccacag atttcggaac tcatctgaag
    14341 cagagtttta tagttggttg agattttttc aatgtattga aatcgtttca ctgtcacgtt
    14401 tttggcaacc atgctcagat cgtttcggat caaatcgaca atggtgttat gctctgcttt
    14461 ttccttgaaa tcatttttta aaatatcagc tgcattgtca atatcctcat caatggtgcc
    14521 tttcattggg taggaaaata ttttgccctg tgaaattttc acaaacggct ctggagaaaa
    14581 aacggcaaac tgatttttga aatacagttt aaacttagcg ttgccaacac aataaagctg
    14641 ttgcaatgac agattggtgc ttacctgcgt gggctgggta agatttgtca gatatgagtt
    14701 cccgtgctga atatttcgaa aaacaatgtc gaatcttttc tgatatgcga taaaagaaac
    14761 aggcgttttg gaaaattcaa ttttatccgg aatttctaca acaacatttt cgttggattt
    14821 tccctgcaaa ttgtacatga tttcagacgg atctatttcg ctcaatggaa ttacgagaca
    14881 ctccgacaga gcgtaatcca gcacaaacag aaacggaatg cccttctgcc ccagtctatt
    14941 catgctattt tgcgcgtctt cctttttcat tgtacttttg caaaagtaaa taattcccgg
    15001 ctttgaaaat tctcctgatc gacaatcacg attctttcac tttcaatctg gtgcatttgc
    15061 tgcgctcgtt taatggtatt gaagtcgatg taattcctgt cgatgcattg caattggagg
    15121 acgttgcgcc ttattcaaaa attttatttt cacccggccc cggattacct tcggatcggc
    15181 cactgatgcg cgaaatcata ctgcgttatg cgcctgaaaa accgattctg ggggtatgtc
    15241 tcggacatca ggccattgct tgttcatttg gagcagagtt gggtcaggtc acacccgtgt
    15301 atcacggagt tactgcaaaa gtaaaaatac tcaaccgcaa ttgtcgttta ttgaatgggc
    15361 tgaacgatgg atttgatgcg ggtttgtatc attcgtgggc tgtgtctgaa actcattttc
    15421 catccaatct cgaagtgact gccatttctg aaaaaggaat catcatggca ctgcaacacc
    15481 gaaaatacga cattcacgga gtgcagtttc atccggagtc aattatgaca cctgaaggga
    15541 aaaacattat ttcaaactgg ctgtcaaagt agattttgga cacgccaaac cgcaaataac
    15601 tcaatttaca cttcagaatt caataaaatc ctttggcctc agattgattt cttcgctcca
    15661 gaaatgggct tcaccatgcg tgatgcgaaa gagcacaatt ttcggacttt caggtctcga
    15721 aacgaatgcc tgcaagtgcg gcttatcctc catcaccttg tttttgtaag ccggatccag
    15781 aataatttca gcttcgccgg caatccgcag catgagtaaa ggatttgttt tgttccggat
    15841 gaaacagact tcaacttttg gattacccat cagctgactt gggatttcct taatggtgct
    15901 ggtctgaaaa taaaaaccgg tttcatcggc aaaccacata tccattgccc tcaccctcgg
    15961 ctgttttcct tcggcagtag ccagaataca aagcggattt tcattggcga atcgtatgca
    16021 gtcatgaatg tccatggttg ctgtttttat cagtttcgac aatatctata aagatacgaa
    16081 tacaaaaggg tgaaatcaag tattcaacaa ccaaataaaa ttataaagtc aattcactct
    16141 ttaaaattca atgcgcggtc aatgtgatca aagaaagggc gggcagcctg gaatgtttta
    16201 tacacttcct cctggaaatt tttctgcaaa acctgtctgt cattaaaaga acgaagcacc
    16261 agaaactgct tgaaacgcag ccattcaatg gccggatgct ccggtgaaaa tcctttgggt
    16321 gcgcgttgta gtttttcgcc tgaaacttcg ccgaaatatt ttttaaaatc cggtttcttt
    16381 acaatttttt caagctcttt ggtgctgtag gccatatcat cgcggatcag ctttaattgc
    16441 gccgaatcgg gcatgtagat acctccgcct gcaaaaatat ttccgggctc aaaatgaata
    16501 taaaactcac agttgcggtc ctccggggca cgaaaaacca tagccccgag ataggtgcga
    16561 tacggcgttt tgtctggcga aaagcgaatg tcgcgataga ttcggccaac accacgcggc
    16621 gggtttgtaa gtatccggtc gtcggattgc ttcatcattg caatcagctc tccaagccat
    16681 tttccaatgt tctcacgcgc cgcgacataa cgatgtttct gcggggcaaa ccattcgcgg
    16741 ttgttatttt cttttacttc gcgcaggaat tgcagggtgt cgggatatat atttgtcatg
    16801 gcagttgagt tgaaatgtaa aattacaaaa caaatgtctt cattttctta aagctgttaa
    16861 atatcaattg ttacaagcgt tacgctatac agacgttaca atcgttacgc gttacagtcg
    16921 ttacagtcgt tacaaagtga cagggtgaca agggtgataa aaatcatttt caaattttca
    16981 aattagcaca tcagcacatt aaaacagttc actcaattcg agccagcgca attcaagttc
    17041 ttcgagccgg tttgtgatgt cggagtattt ggtcgatgct tctgtaagct tctttgggtc
    17101 gtcgtttcca ctgttcatga attcaagcaa tgcagctttt tctttttcaa gagcagcaat
    17161 ttctgttgtc agtttttcgt attcctgttt ctgcctgaat gttggtttat tcgattgcgc
    17221 cttgggttta attggtttct cctcaggttt tgcagcaacc ttttcaaggt tttcctcttt
    17281 cagtttctgc agacggtagg cgctgtaatt ggaataacaa tccttcactt ttccattgcc
    17341 ttcaaagaca aaaatatgat cagcaatttt atcaagaaat gcacgatcat gagtagctac
    17401 taataaacaa ccgggataac caatgagaaa ttcttcgagt acattcaagg tgtcaatgtc
    17461 taaatcgtta gtgggctcat cgagaataag aaaattcgga tttttaatca gtgtaagaca
    17521 cagatgcagt cttcgttttt cgccaccact cagatttgaa tagtaattat attgtgtatt
    17581 atggtcgaat ccaaaataat gcagaaactg cgatgcagac accgaagggg cgtttcccat
    17641 tacaatttct tcggctacat ccttcaccac ttcaatcact ctttttcctt ccttaatgtc
    17701 gggattcatc tgcgaaaaat atccgaagac aatggtctgg cctgttttga gacttcccag
    17761 atcagggcgt tcttcgccca tgatgatttt aaaaagtgtt gtttttcctg aaccgttcgg
    17821 acccaccacc ccaacacgtt cgcctcgttt gaaaatatgg ctgaaatcat caatcacggc
    17881 accgaccggc tcatacgatt tatgaatatt attgatctcc agaatgttct ttcccatcct
    17941 tctttctttc acctgaaatt ctgggcggac atcgtcaagg cagcgtttgg cctttatttc
    18001 cagctcatag aaagattcaa tgcgcgactt tgatttatgt gtacgtgctt ttggcatgcg
    18061 tcgcatccac tcgagttcgg tgcggtatat gttgcgtgcc ttttctgttt cgagacgttc
    18121 ggacgcttcg cgctccgctt ttttctcaac aaaataggca tagccaccat cgtattgata
    18181 catgctgcaa ttgtcgagtt caaaaatttc atcgcataca ttgtcgagaa aatatcggtc
    18241 gtgcgtgacc agcaacagcg ccattttctg cttctgaaga aattcctcga gccattcaat
    18301 catatcaata tcgagatggt tggtgggctc atcaagaatc acaaaatttg tttcatcaat
    18361 aagaacgcgt gccagtgcgg ctttgcggac ctgcccgccc gacagtgttc ccattttgcg
    18421 attcagatcg ccaatgcgca atcggctcaa cacttctttc acccgcgaat cgtaatccca
    18481 ggcagcaagt gcatccatct gcgtggtggc ctcgctcagg agtcgctgat tgaaaacaga
    18541 aggatcgtga ttgacaagtt ctacagcaag ctcatattca cgggtagctt tcacatacag
    18601 attgtccgtc gtgagtacca catcaagaat actcagctct ggatcgaact gaggtgcctg
    18661 aggcagatat gccacgcgga tatcgttgcg catggtcacc gtgcctgaat cgggaatatc
    18721 gagacccata atgatattga gcagagtgct tttacctgtt ccgtttcccg caatgagtgc
    18781 gactttctga ccttcgtcga tgccaaagga aatattctgg aacaacagct tttcgccgta
    18841 ggacttggtc agattttcaa tggaaagata attcactgcg agattttttg caaaggtagt
    18901 gaatcatgaa caaggaacaa ggaagtttga aggatgaagg gaagacacat tcgtgaatca
    18961 tgaacaagga gcaaggaagt ttgaaggatg aagggaagac acattcgtga atcatgaaca
    19021 aggagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg aagtttgaag
    19141 gatgaaggga agacacattc gtgaatcatg aacaaggaac nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn catgaacaag gaacaaggaa gtttgaagga tgaagacaag
    19321 acacattcgt gaatcgggaa catggaacat ggatgtgtga aggatgaagg gaagacacat
    19381 tcgtgaatca tgaatactga acaaggaggt gtgaaggaag aaaggaagac acattcgtga
    19441 atcatgaata ctgaacaagg aggtgtgaag gaagaaggag tggttgattt aagaatatcg
    19501 atcggtgccc ttcgacaagc tcacgataaa atttgagatt tttgatctga tggaagaaat
    19561 gattaatcgc gaatatttca gtaataataa aagtattatg tgcaaatgca aaacaacctt
    19621 attatttgaa aatcaattat gacatcatgt tttcattttt ttattagatt tgtaaaaaat
    19681 ccagactatg aatttcaaaa gctttctgtt tgcatcgttt tttctttttg catccatgct
    19741 gcaggctcaa accgatgcat cgggctcatc tcagttgaaa atgggactgc atggtggttt
    19801 gaacctgagt aaatatcaaa ccgaacttga cagtaccgat gccagtgcac gcattggctg
    19861 gcagggagga atcatgctgc gctacggtgg aaatttcttt attgagggca atctcaatct
    19921 tggacaaagt tcggccaaca tgatgacaac agactccatt ggcacaatag aaaccactgt
    19981 ttaccgcact ttcatggctc tccctgtcat ggctggaaca aaaattttca gcagcgatga
    20041 cggcagcagc tcaattcgtt taatggccgg catagaactg atgtctgttt taaaaacaga
    20101 tgtaagtgat tcattactat acatcgaagc agatgatttc aaaccttatt ctctggctgc
    20161 ggtgggcgga atcggcatgg atctctggtt tctgcgattt gatctggctt tgcgctatgg
    20221 attaacaccc atgctcagca ccgacccgga ttcaaaaatt gtgatgggaa cttttaatat
    20281 cggcgtcatt ttctaaaaca acaatactat gaaattcgaa ttacctcctc ttccctgggc
    20341 agccgatgcg cttgagccca tcatcagtgc caaaaccatt gaatttcact atggcaaaca
    20401 tcatcaggcc tatgtgaata atctcaacaa cctgcttccg ggttcaccct tcgaaaacgc
    20461 gccactggag cagattgtag tagaagccga aggtggcatt ttcaacaatg ccgcacaggt
    20521 gtggaatcac actttttact ggaactgtct cacgccaaaa ggcggcggac aacccaccgg
    20581 agacctgatg gacgctattg tgcgtgactt cggttcgttc gaagagtttc agaaacaatt
    20641 cacgcaggcc gctgcaacgc tgttcggaag cggttgggct tggctcagcg ccgatgaaga
    20701 cggcaagctg attatttcgc aggaatccaa tgcaggcaac cccatgcgca aaggcctgga
    20761 accactgctt acctgcgatg tgtgggaaca cgcctattat cttgacaagc aaaaccagcg
    20821 ccccgactac attgcagact tctggaaatt agtggactgg gaagctgtgt cggataggta
    20881 ttaaaagtag tcagtagtag tcagtggtca gtggcataag gcataagtcg acaggcataa
    20941 ggcataagga gcaatcggag tggcttatgg gtcgtagtca ttattgatta attgattatt
    21001 gtatgctttg agagctggct ttgtgcaaca gagcatggct tgtcaatttt attgaattgc
    21061 aggaatatat gaaacaggaa acattttccc ttcggggaca ggaatacatt caactcgatc
    21121 agctgatgaa actgctgggc tggattgaaa gcggtgccat ggcacacaaa gtcatcgatc
    21181 agggactggt gaaagtggac ggtgaaaccg agctccgcag aagaagaaaa ctgcgcgaag
    21241 ggatggttgt ggagtttgaa aaaatcagaa ttaaaataaa acgatgaaca caatgaaact
    21301 tttttattta cttccctttt tacttatttt ctcttgtgca tatggacaaa acacaaaagc
    21361 tcttaagctc tttaataaag gaaaaattgc ttttgaatcc aaggattatc ttaaggccga
    21421 aagcttcctg caaaaatcaa ttaaaaaatc tgcatctgac ctggcttatt attacctcgg
    21481 gatgactcag ttaatattag acgatacctg cagtgcatgt aattgcttgt tgaatgcaaa
    21541 aatccggggg aattcggatg caacaatttt attcagcaat gagtgtgttc agtgtgacac
    21601 cattaactat cgtggcggtt catatttttg cgttgtcact aaatggacct gcaccaatga
    21661 actggaggcg gaatttttca agagatcaaa aaaaatcgga tcagatacta tggttttcct
    21721 tgaaaatgat tcagctatta atagttttct tttttattcc tctgaatttg aaattgaaaa
    21781 gtatcttgat aaaaccacgc caggtattgt tcctgaagtg cctgaatttc caggaggtga
    21841 atcaggatta atggactttt taagagataa cataaagtat ccggcttatg ctcgcgaaaa
    21901 aaatattcaa gggactgttt ttgttagatt cgtaatacaa aaagatggaa aagtttctct
    21961 tgtaagagta ttaaaaggcg ctggaggagg ccttgatgaa gaagcgatca gagttatcaa
    22021 agctatgccg aattggaaac caggaataaa agatggaaaa cccgtcaata ttatttacaa
    22081 tattccaatt cgattcatat tagcaggctg attttgactc gaaaaattat gcacaagatg
    22141 ttgtctggta tcaaagtcag gcgatttatc ttgcaaggat gaaatctgtt attttcaaaa
    22201 accatcaatg gaaaatgaat ttccaaattg atggtcgttc aaaatatttc cagttacttt
    22261 gtcttcactt catacgaatg caaaatggcc tccagctgca tgagcaggtc gcgtttgtcc
    22321 atgcttggat aatagacata gccctcgagt gtgatcactt tattggcttt tttgtcgtgg
    22381 aaggtatagc tcagaaacgg gccacccata aagtctccga ctgttttcca gagtccgcgg
    22441 gcttcgatgg cgcgcgtatc ggcaaaaggc acattgcgca taaccggagg cgcaatggta
    22501 tcggttgaca tgtatgaacc cggcaattca ccagggatgt tttgcttgac aatcatattg
    22561 cgataggcaa taatctgcga aggattaaac tgggcgctgt ctgtaaatgc acgagaataa
    22621 atcagaatgg cctgagaata tttagcagtt tcgaggcgga gccatgcaaa accatcgttt
    22681 gatttagcca tataataact ttccggaatc acaagactaa aaccgaattg tttttcaatt
    22741 ttttcacaga gagcaacatt ctgagtactg ctaaaagcca gaatcaggcg ctcacgctca
    22801 acttcacgaa gcatttcgac aacagaacca gcacgttcat tcaacaaacg gattgcttca
    22861 gaatcgcttt tggcatgcaa atcaataata agttgtggtg acgaaaagac atcacggcgc
    22921 gctaccaatt gatttttttt cacggtatca ctaacaaaaa aacgaatggt tttccggtta
    22981 cgttcaaaca ggcctttgta atcctgatgc tgaatctgta aaggttcaaa caaaggttca
    23041 gactgattca ggccttcctg ttcggccatc agcatagatt ttacagccgc accaaccgaa
    23101 gtttcccatt gctgatttgg catcacaaca agcacttcgg caaccgttcc atttgaaggc
    23161 ggcaagccag attccgtttt tttaaaacac gatgttatca gtgttgcaac aacacctacg
    23221 gctaatacta aaaaaattct tttcataatt gtcagctgtt tttgtggatt ttgagtttct
    23281 ggccaaccca taaattatcc gatttaagat tgttgacctt tctgattgca tccacgctaa
    23341 ccccattggc attggcaatc ttccagagtg tatcgccttt tttaacagta taatacactg
    23401 tttttaaact tccgttgctg gtatttgaaa tatgattgct gctattggct gttttcccat
    23461 aaacaagcaa tttctgcccc ggataaataa ccgttgaaga caaattattc caggcctgaa
    23521 tgctgctgct gctggtgcca tacatagaag caatggttcc cagcgtttcc ccgcttttaa
    23581 ccgtgtgata attttttgat ccggaagtat tgactgtggt attgttgttt acagtggtat
    23641 tgttgacagg atttgtttta acggttcccg tagtattcgt tgcaatgagt gcgctgtcgg
    23701 cctttgcaac cttgtgaata ctgtcataca tcagaggttc tttctcgaca aaagccaata
    23761 catatttctg cggcaggcga atggcgcagg gattgttttt ggtaccggga ataatttttt
    23821 tgatatactg tggattcaga aaaaccattt cagattcatc gataccaaca aatgaagtga
    23881 agaaattgaa tgacatagtt tccgtcaccg aaacggtatc gagttcccaa tccataaact
    23941 gcgggcgctg gatgttgatg ttatgctctg cataataagt aataacataa cttgcagcaa
    24001 taaaagccgg tacatatgcc tgagtttcct tcggcagaag atgttttatt ttccaatagt
    24061 tggtttcgcc gccagcacgt ttgatggcac ggtttatatt gcctgggccg gcattgtagg
    24121 ccgcaagcac caaagcccaa tcatgataga tcgcatacaa atcctgcata tgatgtgctg
    24181 cagcttcggt tgctttcaat gggtcgtagc ggtcatcaac ataagaagta acattcagat
    24241 catacagcaa tccggtttta tacataaact gccataaacc tgcagcaccg gcccacgact
    24301 tggctgtagg attcaatgct gattcaacaa ccgccagatg tttcatttcg agcggaacct
    24361 gatatttatc gaaggcctgt tcaaaaacag gataataaag caacgacaac cccaccaaac
    24421 gtccaataaa atatcgtttt gaaatataaa attcaatcca ttttttcact tccggattgt
    24481 aacgatattc aaacggcgag gcctcatcca tggctttaac ccttgctgcc aatgtgctgt
    24541 cattaaattc ggggacataa tttgccggaa aattatactt gtttttttcc ggaaaaaaat
    24601 tgtcactgtt ataaaatttc tcacgggtca tttcatcgaa gatttttgca atctccgtat
    24661 tctcaacatt gataacggta tcggttggct gagacataac tgcaaacaca ggcaaaaaga
    24721 acagaaatat gactatgaga tatttcatcg gcaaccattt ttggggtttt aaaacactaa
    24781 agtagcaaat tttattaata taattaacgc ttccggccat gattttatta aatgcgcatg
    24841 gttaatttcg gcagaacagg ccgcaaatat cagacagaca cgttcaaaac attatattta
    24901 atgcgccttc cggcgaatca atatccggaa agccattctt gtaaaattta acaattaata
    24961 tccggttttt tatatattta ccgattaaaa accaaacgta tgtcggattt attacaggaa
    25021 tacaaagact attaccgtgt tcgggccgac cgttacgcgg aaaacaacaa ttacaaaaac
    25081 agctacgaag ctgagaaaaa tctgagtgaa gccatacagg gatgcaatat tttagacgaa
    25141 ttcaaagatc ggcttggcaa tctcaatcac ctttgtgcca ttgcactcac caaggacaaa
    25201 aatctgatgg aaaaagccat ttgtgaggaa ctgaatgaac ccatacgggg caacattcct
    25261 gaaagaattc ttgcaaaagc tgatcaatac accgaagtct ttgatctgat aaacatggtc
    25321 aacgaagaga atacacgcgg catgcgcgaa atatcgctcg acgaagccaa ccgtattttt
    25381 catcattgct ggatgttgct tgatcgcatt gaaacctact ctgaggtatt ggtgcctccc
    25441 gcttatcagg ccgacatgaa aagcaacgca gaatattaca ggaaaaggat tcttgaaatt
    25501 attaaagaca ccgagcaaca gatgcaaatg ctcgatcccg catggaaaca taatcccgac
    25561 atcataaaag aataccggca ccgaaggctg ctgccctatc gggatgagca tattgatgaa
    25621 caactttcga aatataaaac catcgctaac atttaataca agccgctatg ccactggatc
    25681 ctatgatgac cgatgccctg ctgggcgcat ttaaaaacat ggtacaggaa tgccgcgaca
    25741 aaaatatgag cggtgaacat ttcgaccgga tgtgtgaact gtacaatcat ctcgaagagc
    25801 ttacacaaac acacgacgat gtaattgcat tcaacatgca ggtgatgaaa gaagatattt
    25861 atgtaaaaat aagcgattgc tacagccgcc ttctgagtgc tcaggcacag gaagaacaaa
    25921 aaaacattgg ctacgacgac tccgctttac tgaaacaaaa tctgaatgca ttgcgcgatg
    25981 ccatccggcg catcgaagat gaaaaagaga gagtgcttgc cgaagccagg aactatgatc
    26041 cacacaaagc tatgaacgat acactcgact ttgcctaccg caacgaaggg aaaaaaggtg
    26101 gtcttgtaaa aggcgttgtt gacaattccg gaggtcttga tctgataaaa agtgaaagca
    26161 aaaaaatgct tgattcagaa ctgaaagaac ggccgaatgc ttataacagc gatgttgaaa
    26221 tcgacactct tttcaaaaca gaactatata caaaacccat tgaagaccta ataaaactgg
    26281 gtgaagagcc cggaatgacc tacccacgat atctgcggat tcagatagaa aatggtctcg
    26341 acaaagccat ggaagggagc gcagccacca gagaaggact ggtgtttctg ctcggaatga
    26401 ccgaggcatt acctgcaagt ccacatcaca ttgaatgcga aaaacaaaaa ttagaattgt
    26461 ttgatcgatt ggcttcggaa tcaaaaatac atgtacccaa cagcgatgag cttggttttg
    26521 gcatacgcag aattaatcat cacattgaac cattgaaaat tgaatggact gaaatcaaaa
    26581 gcagatggga agacctgatt tacgatttat acatctggag tctttcgtat tgtccctttg
    26641 caccacagat tcagccctgg tcgctttccg aaaatccacg cgaagccgtt atcagaacac
    26701 aaaaaacaca gcccggcctg cttaaacaac atgaaaaact gtttcaaaaa tattttggtc
    26761 ttgaattctt tgatattttc aaacatccga catttgaatg ggaagtccgt tacaagtatt
    26821 taagcagctc acaggaattt ctggaatttc tgattgaaaa aatatatcct gtctgtattc
    26881 cacttcagga catgccttct gaattaattc cactgcgctc ggcgatgttc aaagacaaca
    26941 aggaaatgaa tccggatctt gacaaaacca tattgccgat ggtggaatat tataattctg
    27001 ttttcggtca aggccgctgg caatcgaaat atcagatgcc tgaaaaatca aaatcaactg
    27061 ctgcggtttg gaactggcag atgtttaaat ataaaaatta agattttttt ggtttgactg
    27121 ctgaagcgcc actggctcct tttgttctga cagactaagc tgtcggcgat aataaatatt
    27181 ccggacatta accttgatct tgacctcgac cttatctccc tgaatcatga acagcgaaca
    27241 aggaatttcg aagtaagaag tgaagtctta agtcaaggat tcttagaagt tctcacttcc
    27301 ttgttccttg ttcagagttc ttgatcttga cctctacaca tctatagctc tgcaatcatt
    27361 tcagcacctc aatgtgctcc agaaaaattt tcagaccaat tccaatcaaa atcaaaccac
    27421 cgattatctg aattttcgga cctaaaaacc cagcagcttt tttcccgata aacattccaa
    27481 tcatggctgt aacacctgta aacagaccaa taacaggtgc tgcgatccaa atatttatat
    27541 tggtaaaagc caatggaata ccaacagcaa atgcatcgat gcttgttgca aatgcgagtg
    27601 taaaaattaa tttcatgtta ttgatatcca tcggatcttt ttcatcattt ccaaaggatt
    27661 caagaatcat tttaccgcca acaatggtta acagaaccag cgccacccaa tgatcgaccg
    27721 gggctataaa ttgttcgaga gaaacacctc ccagccagcc caacgcaggc attactcctt
    27781 gaaacacagc cataataaat gcaatacgaa gggcctgttt aaagcggatt ctgcggtcat
    27841 ttattccaat ggaaatgctg attgccagcg tgtcgaagca caatccaata cttaaaataa
    27901 tcaaccagat acaattcata cttcaaaatt actatttttg agcttgcttt aaacatatgc
    27961 accagatgtc aatctaacac aaaaaatcgc aacatgaaaa aattgttttt tgttttcttt
    28021 gcattatccg cattcggatt aaccgctcag aatttttcca tcacaaacta tacaacgtcc
    28081 aacagcccga ttggaagtaa tagcattaca gctctgaaag gagactcgaa tggaaatatg
    28141 tggataggat catccgacaa cgggttaacg gttttatcga gcagcacagg gaccattctc
    28201 acaaccactg acggacttgt cgggaacaat gtaaaagata ttgatattga cggactggga
    28261 aatgtgtgga ttgccaccac cgccggactc tcgaaataca atggcagcac atttacaaat
    28321 tatactacag cacaaggatt gccaagcaac gatcttcgat gtgtatttgt tgatgcaagc
    28381 aacaatgtat ggattggcac ttcggcagga gtatgcaaat acgatggatc gtttactact
    28441 tacacaacca ccgacgggtt gattcacaac actgttcttg ccattacgca ggatttatcg
    28501 ggcaatatgt ggttcggtac tgctgaaggc gttaataaac ttgcaggaac catctggaca
    28561 gaccacacgt tcctcaatgg tgtcaacgcc aacggagatc aggtgcaatc tgcaaccacc
    28621 gatgccgacg gaaacatttg ggtaggatca ctcccgagta tgggacttgg tggcggatta
    28681 tacaaatggg atggtacttc atggactact tacacaacca ccaatggact ggcccacaac
    28741 gatgttcgtg gaatcgcatc agatcccatg agccgtatat ggattgcaac caatggtaac
    28801 ggaaccagtc atttccgcaa cgcaacattt acaacatacg gaaccagcac cgggataatc
    28861 tccacttctc aaacctgctc cgaagttgat tataacggct ttgtctggat tggaaccaat
    28921 gcgggcattt caagaatttc aacgctcgtt attcaaacca gcatcactac caatgcccat
    28981 tgcggcaacg atgacggatc gctgacaatc aatgttggaa ccatcaacgg tccggtttat
    29041 tttacctatg acaatggagt aacaataaca accaacaaca cattaaacaa tctcaccgct
    29101 ggcgtttatc aggttcagct caccgatagt tcaatttttg catcaatggc tattgaaata
    29161 tttagcagta ctcaagccat ggacgccatc accgaaacaa cctacaatct atgtgacggt
    29221 gactcaatgc tacctggtgt cgatcccgct tttaccaatt gcctctggga tcctgcagca
    29281 gtagtttctg atgccaacat cccaaatccg ctgatttctc ccactaccaa catgtgggtt
    29341 tctctcacct acactgatac caacggatgt gtaggcgaag attcaattca gttttttatt
    29401 atgcccgttc ccaccgtctt tgtaaacatc acgaatgact ctgtatttac ttctccgctt
    29461 atattcaaca actatcagtg gtttttatat ggcgacacca ttccgggcgc aacaggacaa
    29521 agttatactg ccactcaacc cggcgtttac atgctttgcg caaacaacac cggacaatgt
    29581 gttggatgtt ctgatcccat caattttcaa aacgctggtt ttgacgaaaa caatgattgg
    29641 gcaaaaatct tcgcaaacga cggaatccta cactggatgc tgccttcaga aggatgcatg
    29701 atcagtattt atacaccgga tggccgtctt ttacaacaat ttattgcttc tgataaaaac
    29761 ggaactctga cattgaaaaa caatgtgtcc ggcataattt tgattcaggt tgaaaatggc
    29821 gacagaagcg catggataaa agcgtttgca aaataaccca atatttattt tcgaaaaaag
    29881 gcccgcaaaa ctgcgagcct ttttttattc tggttgtatc aatcagaaat tggcacgtag
    29941 cgacaaaatg aaatttcgcc cgggagcaac aatacctgaa ctgtaaggcc ggtagcgctg
    30001 atctgaaata ttttcaagac ctgcacttac tgtaaatgta ttattcatct gatacattgc
    30061 tttaaaatta agcgtgtacc atgccggcga atatggattt ccattttcat cctgagcata
    30121 gatttcggtt ttgcccttct catcctcagg catattttca aacgatacct cggcacaata
    30181 atttgcataa aactgcaaat cgagttttcc ataatgatat gaaagccgtg ttacgccaaa
    30241 acgaggagca gcatgccgcg atgggctggt agtgccatca tcaagctcct ctactcccac
    30301 ctgaaaatta tagtctgttt caaaaccaaa acccttcggc aatttgactt caatgccggc
    30361 ctgcactccg taaacattgg ctacggctgc attctgaatg gcctgcacct gactgagttc
    30421 accatcgtac atgatgctgt caaggccgtt caactgaaaa tcgcggcgca ccatggcatt
    30481 ctgaagaagc gtgtaatagc cgcttgtata aactttcaga aattctccaa aaactttcga
    30541 taccccaata tctgcattat aagcgtattc ggcctcaaga cccggattgg gaactatcac
    30601 ggaacccggc tctgaatcaa acacttttcc catatcatcc acattggggg cgcgaaaagc
    30661 agtagcaaca tttgccgtaa caacccactt gtcgaatggc cgcaaaacaa agcccagact
    30721 tcctgtaagt gcacctttac tgagttcggc acgtgtatat ggaaacggat agaatgtggt
    30781 atcgaaaacg gcctcaagat cataagcact gtatctgagc ccaccctgca ccatgaactt
    30841 ttcggaaacc ttgtgctggt aagtcagata tacaccatac gaagaccagt ctgattgcgg
    30901 ataacgggtg gggccatcct gaacagaatt ggttacaatg ttttcatcaa cgccaatgga
    30961 agtcacctta ttccacacat attcagcacc ataatacaac gtgtattttt taccaatgct
    31021 tttattgaaa tcaagattga cagaatacgc atccactttt tcgatgcgcg tttcacgatc
    31081 cgctttatta atgtcgcggc taatgcggct ttcttcaaaa tattgctgag ccaaacgcag
    31141 agacatctga tcgaacaacc ttgttttttt attgtgggca acactaaggt tattcatcat
    31201 ccatttctgc ggaccataaa accattcggc ataacgcggc aaaccattct tgtaacgtat
    31261 aagccggtcg tagcgcgaat attcagatgt ctcggaataa tgaaaaccat attgaacatc
    31321 ccatttttca ttgggcgaaa aacggatttt ctgcattata ttcatttgtg taaaaccagt
    31381 cggagtctgc acgcggggat cattgttggt cataattcga tccacactat cctgacgctg
    31441 aacataataa ggttgcagat attcatcagg gccgttgctt cccatcttca gatctccata
    31501 atcaaaagta ctgatgctgg tgagcatcgc ccattttttc cagccgacat tcacatcaaa
    31561 atggccggtt ttttcattat tggccgaaga atagcgcgtc acggccttgc cagtgatgta
    31621 gggctgatcg gtcatcgaaa gctttggcgt cagcgtttga aaactcatca cgccgccaat
    31681 ggcatcactg ccgtaaataa ccgaaccagg tccgaaaaaa acctcggtgt tttcaactgc
    31741 aaacggatcg agcgagatca cattctgaat attgcccgca cggaagatgg ccgtattcat
    31801 gcggactcca tcgacagtgt acaacagccg gttggttgca aagccgcgta tcatggggct
    31861 tccgccgccc tgctgacttt tctgaataaa cactttgccc gaagatccca acaaatcggc
    31921 cgctgtctgc ggattctgca aagcaatgga tttggacgat atagaaatga tagaagacgg
    31981 aacaccggat gaaacctgat tccatcgggt agcagataca acaacaccat cgatgttgat
    32041 gcccgtttgt gtcatcataa caataaaacc agaggcctca atctgagcat agctaagcgt
    32101 ttcagtttta tatccgagaa tacggatttc aattttctcc actcctttaa acgcacggat
    32161 atcggcctgt ccttctggat ttgtaacagc aaatgcatgc ggctgtgcgc ttgtgagcgt
    32221 taccaggtcg agcggagaac ctgtctggcg gtctttgact gtgaccacct gcgcctgcag
    32281 gcatgagaaa agcacaatgc ctgcaaaaaa caatacaatt tttttcatta ttctttctga
    32341 tttgattaat aaggaaattg ttccgaagta aaaaaacccc ggacaaaatg agataatata
    32401 aatcagatca gacgtggcgg cgggctgatt acatcacttt tttcgcaatt gtaaataaaa
    32461 ccgggattaa taaaaataat gtcggaagaa ttgtttacaa cggaaaattt ttctattgtc
    32521 gccggaataa aagtaaatat ggttgtaaac tgatgcaaca agacagccag cgggctttga
    32581 tcgttatccc ttgcagattt ttcaaactgc gcaataagaa atttttcatt cgaatcgcgg
    32641 taagcaaaaa gaaaaacact atctgaagaa atttcaacat cttttacatc gtaaaactca
    32701 ccatgtagaa taatttcatc atccttatcg ggtacacgga aaaacggtaa tccatgcgaa
    32761 aagtcgcttt ttgaaaaagc aatcacatca gcttcggatg cagccatttc gcgcaatgca
    32821 gctttggcaa gactgcgcac gtgcaggcga tacgccttaa aaagcaagaa ctgcccacct
    32881 gcaacagaaa gcagaaaaac aacaagaaca gataaagcga atactttttt cacgatgtaa
    32941 tgtgaaaaca aaagtaaaaa aaacacggat tattataaaa aaagagactg gaaaaccagt
    33001 ctctttttta acaatgcatt ttatcccatc agcatgtgct ccaacacata aaaaccaatg
    33061 ccggcaagat atccaattaa agcaagccag gaaatgtgct taagatacca tataaagtca
    33121 attttttcga gacccatagc tgcaacacca gctgctgagc caatgatgag catacttccg
    33181 ccggttccgg cggtataagc cagatattcc cagaacaagc catcctgaac aaaattggcc
    33241 agataacccg tagcaccagg atcagctatc gggtacatac ccatggcacc agccacgagt
    33301 ggcacattat cgaccaccgc tgaaagaacg ccaatggcac caccaatcag ataaacatca
    33361 cccagactgt catcgagggc ctgcgcaagc acgctaagct gaccagccga ctgcaaagcg
    33421 gccacggccg aaagaattcc aaggaaaaac aataccgttg gtacatcgac ttttgtcaga
    33481 atagatgtaa cactgagctt ttgttccttt ggttttttga aatgaagaat ttccgtaatc
    33541 atccagataa tggccagaga aagcagcatg cccatatacg gaggaagatg tgtgatggtt
    33601 ttaaataccg ggacaaacaa cagtccacca actccaagac caagcattaa attacgctca
    33661 aatgacgtgg ttttggattt tttttctgca tcaatttttg gtctggtaac atttcctttc
    33721 atggtcatgc ttaacagcag caatggaaca accatacaaa ccagacttgg aataaataca
    33781 ccaacaataa tattaaccgt tgttacctga cctccaatcc aaagcataat ggtagtaaca
    33841 tcaccgatgg gcgaccaggc accaccggca ttggctgcca gaacaaccat actggcatag
    33901 aaccatcgtg tatgcttgtc atcaataagt tttcgcaaca aagcaaccat aacaatggtt
    33961 gtcgtcagat tgtcaagcat ggctgaaagg aagaaagaaa taaaactgat aatccagagc
    34021 agctttacct tatgcgtagt tttaatcctg tcagtaataa ccttgaatcc ctcgtgctga
    34081 tcggtaattt caacaatagt catggcgccc agcaaaaaga aaagaatttc gcttatttcg
    34141 cccagatgct cgataatctg attatgaaca ataaaatgct gcattccttc aattgtattt
    34201 gcttcgggat tggcagcaaa atactcattc caggcggtac ttactccact tgtaaagata
    34261 tcaaaggcac ctgaaatgta aacgacccag gttaatgagc caatcaacaa agctgaggca
    34321 gccttgtcta ttttcagcgg gtgttcgagt gctatggcaa gataaccaag aacgaacaca
    34381 ataaccatca gtaaaaacat gtccataatg agctgtgttt aaatttggcg caaaagtata
    34441 aaaagctttt ttaaccaacc ccattgcgaa gtggttaaaa ttacgcttct ttaactgatt
    34501 aaaatcattc gaccaaaact aacacctcag aacggataac cgattccaaa acttaaggtt
    34561 gtacttttca atggtttatc aaatccaacc cacatttcac catccggaag agccggatcc
    34621 ctaagcggga aagaagcatc gattctgaaa ataaagaaat caaaatcaag ccgcagtcct
    34681 aaaccggtac caacaccaaa ttgtttgtaa aaatcgaggc ccggaaactc gcctcccgga
    34741 aactcctcgc tggcattttt gagccataca ttgccagcat cgacaaacaa agcgccgttg
    34801 aaccaactaa taatcgggaa gcggtattcg aaattaaaca ccatactgat atcgccggtg
    34861 cgatcaaaaa aagtgctgtc ggtatatgta ccaggtccca gcgaacggaa tttccaggcc
    34921 ctgatgtcat tggaaccgga agccgaataa cttttctcaa aaggcagcaa catgctgttt
    34981 ccaaaaggaa taccaaagcc aaagaaaaaa cgactgacaa aggtggtttg attcaaccag
    35041 ggtttataat atctgaaatc aatatcaact ttagcaaatt gcgagtaatt aattcccaat
    35101 actctgtata tctcgccttg ccgggcgctg ttgaaactat ttccaggcca caacataaaa
    35161 acacctgcct gctcaatcag taatttccag taaaaatacg atctgttttt tcgcaaggcc
    35221 tgattgcttt ttatcattga aaaaccacaa cccggaataa agtgatcttc ataactgtag
    35281 cgaatggttc tcgaaaactg tgaaatgcgc tctgcaaata acgaatcagg caaaatgcgt
    35341 acaagattga tgggtaaata aaactgcatg accgttgttg ccgattcttt ccattccagc
    35401 gttgccttca aatggctgat atatcgctca tagtcggggc gcaactgata actgaatccg
    35461 gtagaatatt tcatcatcgg gtgactgctc tgagtaaacc aactattgtt tacaggcgtt
    35521 gtcaatcctg cttctgtact aatttcagcc gagttaaaag gcaagccgct aataacatca
    35581 tttttttctt caaaaacagt tttctgcaat tcaagtccac ccttaaacct gaaattgaat
    35641 tgttcggctc catgaaaaag attgcggttt ttataaacca gattgccggc tgtgccaaga
    35701 tttccggatg aattggtgcc ttcaatttca acattgactg cctgaacagg cgacaaacta
    35761 agttcaacaa aagcatcgag cggaatgggt tgatcgggtt gaactccggc tgtatcggga
    35821 acaaagcgga tgttgatgta tttaaaaata ttcagactgc taagctcagc ctgcgtttta
    35881 ttggcattgc tcaaattgaa ataatggtct gtgcggataa aaatctttcg tgcaataacc
    35941 ttgggtttca cacgcattgc ttcctgatac aaaaaacaaa gtgtgtcagc ttgttttcga
    36001 ttgcgaagat gttttacata atgtaatgta tcgtatttca caacaccctc tgaagtgact
    36061 gagaaatcgg gcaaaacaac cagctttcgg actttatatt tacggtgatt ataaaattcg
    36121 gtaacgccgt ttacttgttg aaaaggcctg ttgattataa tctttacatt caattgtctg
    36181 ttaccgatgg ttgaatcaat ctcgtagcgt atataagatt tgctgaatgc aaaatatcca
    36241 agatttttca agtcgtcatt aatacgcata cgttcacttt caagcacatc ttcattgtaa
    36301 atatttccct gttgtaaaag agaatttgct ttaccagaaa taagataaga attaatatca
    36361 gcatcaccgg aaacaaattc aattttatta aatatgtatg gaacaccagc ttttatgcgg
    36421 tattcaactt tcgccttatt atttttctta ttaaaaatca tcgtatcaga aacctcggcg
    36481 ttaaaaaacc cgtgtttttt acaatacaat tccatttgcg aaacactttg tttaacaagc
    36541 cccgaatcga gaaaaaccgg ttcttctcca acagtattca tcaaccagct gcgcagtccg
    36601 cgttttgctt tttccagttc cttttgttca ttaatatatt tatttggatt tcgtttcttt
    36661 ttcttttcaa ttttttttct gatatcagct tccttttttt tagtacggtc tttcatcaat
    36721 gcacccagat tgtaaactcc tgtatgcagc cgcataccaa gaaactgtct gttttcgtgt
    36781 tgccggatca acccgtcaag gtcgtaataa tccaatgcct tattgtcaat aacaagtttt
    36841 gtatcagtaa gaagaatctt cccgggatta cgacgcgttg tactgcagcc ggccagcaaa
    36901 agcgcagcaa aaaaaaacgc aatgagatga tatttaaaaa agacactact catatctcaa
    36961 tgattcgata ggatcaagtc tggctgccct gttggcggga ataagccctg acaatatacc
    37021 aacaatcagc gttaaagtaa accccagaat aacccatccc cagggaacaa taaaaatacc
    37081 gtcaagatag atactgataa catttccagc caacaccccc agcagaattc cgatcaatcc
    37141 acccatctga ccaaccacaa tactttcaat cagaaactga ttacggatag ccacacgagt
    37201 ggctcccatg gcttttcgca caccaatttc gcgggtacgc tccgaaacac ttacgagcat
    37261 aatgttcatc aacccaatgg aagcaccaat cagcgttata aaaccaatta aagttgcagc
    37321 caatgtaaca taacggatat tttcgagcag catatttacc atgctgtcac tttgacgaac
    37381 atcaaaatta tcggcttgcc ccaccttaag tttccgtatg gtgcggaaaa caccagtggc
    37441 ctctccggtt gcggcctcga gcagttttgt atcgatgggc ataacattga tgcgatacga
    37501 aacatcggac ttgctgaaat acctgcgaac cgttgttacc ggcaccagac aagaattatc
    37561 accagaaaac ccgaatccgc ttcctttggt tttcattaca ccaacaacaa caaaacggac
    37621 agggccaatt gaaatctgtt tgccaagcgg attttcaccg gtcggaaaca acacaccctc
    37681 cacttcgctg ccaatcacag ttacattgcg acccatctcg acttcatgac gcgaaaagct
    37741 gcgacccttt tcgatttcct gaccgctggt aatcatataa ttttcatcaa ttccaagcac
    37801 gccaacattt ggatttgatt tttcgagatt atattttacg gttgaagaac cggatgcatg
    37861 cgtaaaaaca gatactgtag caggaaactg ataccgtttt ttgaatgttg ttgcctgatc
    37921 ccatgtaata tcttcaaatt ccaccctgtc gtttgagctg cgacctctgc taccccgcat
    37981 ctgaacatct gtaatggtaa aagtattgct ccccatacgt gtaaaattct cgctgagcga
    38041 aaacttgatc gattcaattg ccgtcagaat actgataaga gccataatgc caaaagcaat
    38101 aatcagcaca gtcagtatgg ttctcagcaa atgactgcgc accgaccgca atgcaatatt
    38161 aaagctttcg cctatgattt ttaaattcat ctacacaata ttaagtgttg ataatgtgcc
    38221 gaaaatgaac gcccatgaaa gaacagatat acaaaagtaa acaattatac attttgtttt
    38281 tctgaaccaa cgacggaca
//
LOCUS       DAOPKE010000017        37807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1151_length_37807_cov_14.241391, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000017 DAOPKE010000000
VERSION     DAOPKE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_37807_cov_14.241391"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..242
                     /locus_tag="PKY63_03810"
     CDS             117..242
                     /locus_tag="PKY63_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99773.1"
                     /translation="MKIDDYDEERVVDAMIRSEASGYHYLLGEEDFRAILGYMKV"
     gene            286..1044
                     /gene="mtgA"
                     /locus_tag="PKY63_03815"
     CDS             286..1044
                     /gene="mtgA"
                     /locus_tag="PKY63_03815"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPE99774.1"
                     /translation="MATLKKKKRSFFGRIWRFCLKLAMLFFILSIGTTIVYRWVNPPV
                     TPLMIIRLFGQKMDGKDLKLEKDWLDIDSISPNMWHAAIVQEDPKFLYHNGFDWEAIK
                     IAQEYNKTHKNKRGASTISQQTAKNVFLWPDRTWIRKGLEVYFTFLIETFWSKERIME
                     VYLNIIEFGDGIYGVNAAADEYFKNSADKLSKEQAVSLAACIPGPLIMNVKKPTQLYY
                     IKKSFIYRYWGYGPVKELVKHPYSEWEEIYGKKR"
     gene            1028..1525
                     /locus_tag="PKY63_03820"
     CDS             1028..1525
                     /locus_tag="PKY63_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPE99775.1"
                     /translation="MERKGDKNVYNWLEKLDIAYDYYEHPPAPTAEIAAQYWKDIDAA
                     HCKNLFFRNHKGNQHYLVIFHYQQNIMIRDLEQRLKQGKLTFASPERMDRYLKIKPGS
                     VSPFGLIHDKDHHVIVFLDENLQQYDRISFHPNLNEASLVIGFGDFIRYMNAVGNRYE
                     FLSLY"
     gene            1544..2023
                     /locus_tag="PKY63_03825"
     CDS             1544..2023
                     /locus_tag="PKY63_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99776.1"
                     /translation="MRMNDADIKEIKIACRAGIIIPANLFLLSFLIASALFIDNGFTL
                     SFSLIFSLLTTLIISGFVSFLINRNYYLDIRDQEKDESFSIVQKKQHKKDFEAGSGVI
                     AKFLAKPMREFIRYDIIVDNTIYRVDEKFYNECAEGDRIILYFARHSRYHLGFELKK"
     gene            2020..2538
                     /locus_tag="PKY63_03830"
     CDS             2020..2538
                     /locus_tag="PKY63_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99777.1"
                     /translation="MKKYIDNNLGLVFPQPVLIGGVIFIVMGLLVMFEIWYFGILMMV
                     LGLYVVLGVNGARIDVEGRRFKSYSKWLWIRSGQWKSMDPYPYVSVLGKQIGLTLASR
                     GNRTMDVGEITYTVCLLSSTHRTKVIIRAEDTRRAAEKYAAYIAERLGKEVVQYTPEI
                     SAQTRERNMRRR"
     gene            complement(2650..3771)
                     /gene="hemW"
                     /locus_tag="PKY63_03835"
     CDS             complement(2650..3771)
                     /gene="hemW"
                     /locus_tag="PKY63_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPE99778.1"
                     /translation="MSHLYIHIPFCHSKCPYCDFFSVATKKWHGEFVQALCREAKLRK
                     DEIKGKVETIYFGGGTPSLLTEQEFSEITLAIQQHFSIDKYAEITLEANPDDVTPDKL
                     VFWKSQGVNRLSIGTQSFDNKELQYLGRRHTANRSRQALKDAVNAGFDNISADLIHGL
                     PEANIENLFRSIEELSQTGVKHLSMYSLTVEKGTILENHIKKGVLRNINEDEQAETYL
                     RAVEKAESCGFIQYEISNFCVPGFESKHNSAYWKSASYLGLGPSAHSFDGKKRSWNVS
                     DIPQYITGINEQKPVFESETLSATDIYNEFLLTRLRMVCGIQLEEMKQLFGESITEEF
                     IEEVKKLNVFQYLHITEKSLALTRSGLLFADRIISELMK"
     gene            complement(3788..4012)
                     /locus_tag="PKY63_03840"
     CDS             complement(3788..4012)
                     /locus_tag="PKY63_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99779.1"
                     /translation="MTQDNNDYVFSRVLRVIFPRSPELRKLSNQGIGLLLFDEGVYSA
                     GGGVVVFNGTSYCNFWDKRFITFKKLTIGN"
     assembly_gap    4178..4277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4278..5866)
                     /locus_tag="PKY63_03845"
     CDS             complement(<4278..5866)
                     /locus_tag="PKY63_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPE99780.1"
                     /translation="MNFISCIFSIFAINTNPAMSEAIKHECGIAFLRLRKPTEFYLAK
                     YGNPVYALQKMSLLMEKQHNRGQDGAGLACVKLNLPPGKPYINRIRSNSSTPIIDAFN
                     QAFAPLHELKTAQSPRYLDLGYLKENIDFTGEVFLGHLRYGTFGKNNIKYVHPFIRPN
                     NWKTRNLILAGNFNMTNADELFQKLIQMGQHPVETADTVTIMEQIGYYLDEENDLLYQ
                     RYKAEGLSRVEISKHITNELNISNILRKSCKLWDGGYVLAGMIGNGDSFVMRDPSGIR
                     PAFYYADDEIVVATSERPVIQTSFNIHYENVKELLPGQALIVKNNGSYCIEQILEPKE
                     EKYCSFERIYFSRGTDADIYKERKKLGRLIVPQVLEAINYDVENTVFSYIPNTAIVAF
                     MGMYASLLDFTDKVKRDKILALKNPSASAIDEILKLHPRAETIAVKDMKMRTFITTDK
                     ERDDLVDHIYDVTYGLVRNGIDNLVVIDDSIVRGTTLRQSIIKILDRLEPKSILVASS
                     APQIRYPDCYGIDMAKLGDFIA"
     gene            5932..7767
                     /gene="mrdA"
                     /locus_tag="PKY63_03850"
     CDS             5932..7767
                     /gene="mrdA"
                     /locus_tag="PKY63_03850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPE99781.1"
                     /translation="MSENRSYNNRKIFFYFLFSITGFIFIIKLFLIQVVDDKYKESAN
                     NNALRYMYEYPARGLVYDRFGKLIVYNSATFDLMVIPRETESFDTLRLCEIIGITKTE
                     YDKRMKKAINLSGYHQPSILDSDLPADVYSRFQEESSSFKGFYIQSRTKRAYTLPVAP
                     HTLGYIGEASPMIIEKDPYYRSGDYIGINGLEKTYEKELRGEKGLRVMVVDVHNTVQG
                     SYKNGELDREPLSGKDLYSTLDLELQNYAESLMVNKRGGIVAIEPATGEVLAIVSEPS
                     YDPNLLVGRTRSKHYLELQNDVQNHPLYNRAIMTRYPPGSTFKVVNGLAGLQEGIFDD
                     QTMFSCFGGYRLSESHTIDCHAHPSPLAIRSAVAYSCNTWFCWAFKKFVDSDKFTSSR
                     EGYERWRNYIYKLGYGHALGIDLPNEFGGYVPEVDYYDRMFGHKKWHANSIVSVAIGQ
                     GEIGATPLQLANLTAIIANRGYYITPHVIRAIGSEKNLNTKYMERHETGIDKSHYEII
                     ADGMEMAVLSGTATVAQMNGVVVCAKTGTAQDPPRKNHSVFIAFAPKVNPKIAIAVLV
                     ENSGFGATYAGPIASLMIEYYLNRKIERKDLEERIINTVLLNPNQ"
     gene            7764..9026
                     /gene="rodA"
                     /locus_tag="PKY63_03855"
     CDS             7764..9026
                     /gene="rodA"
                     /locus_tag="PKY63_03855"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPE99782.1"
                     /translation="MSQFENKTGKMDFWVLFIYIALMIIGWLSVFATGYREGPFSIFD
                     FSMAYGRQSVWILTSVLMGLAILLIDARFIPRMSFGVYVGVLFLLVLVLVVGTVTSGN
                     RAWIELGAGVKLQPSEFAKYGAALFLAKFLSIPGIKFSNKKNRNAIFMIVLIPMFLVF
                     LQGDTGSAIVFASFLLLFFRIGMPGFFIFLVIYISILFVATLLIKQIVLILILAGIFA
                     LLIYFFREKRKEIIRYLVMFFASAIFIVSVNYIFATVLKPHQKDRINVLIGKEYDPKG
                     SAFNVNQSLIAIGSGGFSGKGFLQGTQTKYNFVPEQTTDFIFCTIGEQWGFWGSSVVV
                     LLYVFLLIRLIMMAEKQRSDFSRYYIYAVAGIFFLHFFVNIGMTIALVPVIGIPLPFV
                     SYGGSSLWAFTLMLFTVLKLNLHRNSVL"
     gene            9029..9589
                     /gene="thpR"
                     /locus_tag="PKY63_03860"
     CDS             9029..9589
                     /gene="thpR"
                     /locus_tag="PKY63_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPE99783.1"
                     /translation="MNPRLFIAFEIVPSEITLREFANIRQIFSRDRFSWAKSGLLHMT
                     LKFLGETPTCRIEAVKEAMKESFEKEASVKFRLESLGVFGSSYAPKVLWTHVSPEDIC
                     RVWFTKLKSSLIKRGFEYDRQNFVPHLTLARIKQIGDKNLLAETVGKYQGFCFNESDV
                     KEIVLYESVLKPTGAEYIKHFSVSLH"
     gene            9614..10348
                     /gene="lptB"
                     /locus_tag="PKY63_03865"
     CDS             9614..10348
                     /gene="lptB"
                     /locus_tag="PKY63_03865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPE99784.1"
                     /translation="MKLRAENIVKRYKSRTVVKGVSFEVNQGEIVGLLGPNGAGKTTS
                     FYMVVGLIKPNEGKVFLDSEEMTDLPMYRRAQKGIGYLAQEASVFRKLSVEDNIRSVL
                     EFTKFTKQEQADKVETLLDEFGLQKIRKSKGIQLSGGERRRTEIARALAVNPHFILLD
                     EPFAGVDPIAVEDIQEIVSKLRDKNIGILITDHNVHETLSITDRAYLLFEGSIIKAGS
                     AEELADDEYVRKVYLGKNFELRRSVK"
     gene            10357..11298
                     /locus_tag="PKY63_03870"
     CDS             10357..11298
                     /locus_tag="PKY63_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPE99785.1"
                     /translation="MRICVLFVSLFLIISAYPQTTKYSVVLHGGAGNFDSKTFSPAQK
                     NNYNDFFESILAMADSMAIAGDSAIKIVTAVIVKMENSPMFNAGRGAVLTEDGIAELD
                     ASVMDGTTLKAGAVAGVRIIKNPILAAQKVMTNSQHVFLAGRGAEQFASLQGLEMVDS
                     SYFATDEMKKKFEKIKADKNGTVGCVVRDIYGNICAGTSTGGMMMKKWGRIGDSPVIG
                     GGTYADSRWAGISCTGHGEFFIRYAVAYDVVARMKYGRQTLKEAMHAVLFDVLLPAGG
                     TGGMIGIDGSGNIVVDFNTTGMFRASTTQSGVHEIEY"
     gene            11355..11570
                     /locus_tag="PKY63_03875"
     CDS             11355..11570
                     /locus_tag="PKY63_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99786.1"
                     /translation="MEHSNTAIIAIALFLYLFGLFSVLFIAITPKMIEISESGKDSPV
                     IKNPAMAAAARPALTMVAQCVFGGSSA"
     gene            12228..20195
                     /locus_tag="PKY63_03880"
     CDS             12228..20195
                     /locus_tag="PKY63_03880"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPE99787.1"
                     /translation="MVFFNAAVLHGVAQCPVNAWFTWTRVCNNSMVHFTDSSFLTGTG
                     QIVDWQWDFGDASPGSSNPNPNHNFPGIGTYTVTLIVTDSSGCTDVATIPVSVDPLPV
                     AEFTFGPLNVCSGNVASFTFTGSGNGLTYEWNFGDGQTSSQQNPSHVFTSFGCLSQNF
                     NVSLTVTDENGCSSTISHQVLVLQQPNVFYFEPNGFVFCHTDTSSISDTIHVYNFSPD
                     IGCIDSYSIDWGDGTPASTSPPELFDGMHPLNHVYTNIGYYIVTITAVGYNGCQTVFT
                     DTATVESNPVASLIGPPVGSNVGCAPLNVCVVNMSQNVSASTVMTVDWGDGVIETLLP
                     SSVGQTICHNYLISGCVAGSMTNYAVILTAQNVCDFSQSSWSPVRVYEPPEAVFNIVD
                     DSVCVGENATFINVSEPNLCAAASATWYTWDFGDGTTSGPTLVPAGSSPQQTIAHAYG
                     DTGVYVVTLTANNSSVNGCGMTSFQGLVYVSQTYAQFSYDTVCFGDPTHFVDSSWALS
                     SSIATWNWNFGDGGTSTVQNPNYTYTNWGDFNATLTVTSDAGCTAVMTHVVHVDTLPF
                     VQFAWDTVCVGDPTTFTNLSYGRGAPIISYFWNFGDGNTSTFEDPTHIYGSAGSFIVT
                     LTVTDANGCVNTILNSVPVSPPPIADFDTDTACVGSASVFTDQSTAPFGTIVSWNWDF
                     GDGSGTSAIQNPTYMYADTGNYNVTLIVETNIGCVDTITLPVYVAPIPLADFTFNIVC
                     AGNTTTFTDASQSYGWFITSWQWNFGDGNTATTQNPTHIYADPGVYTVSLIVANQFGC
                     SDTIVHSVPVDSIPTAAFVANNACLYQTVGFTDLSISHGSNIVGWEWDFGDGTTSVLQ
                     NPTHIYAASGVYSVTLIVTSAIGCMDSASLPVSVYPLPEAGFTMVSACEGDPVSFTDT
                     SLANGGSIVSWQWDFGDGSGTSAFQNPQYFYTDTGIYYVTLIVFNSHGCSDTVINPVY
                     YLPRPTAEFVHDTSCAGQPITFTDVSAGNGSTIVSWTWDFGDGNTSALQNPVHPYATS
                     GSFLVTLIVSSNSGCADTVQHMVVADSLPEPLFTAPSVCFNDTTYFTDMSIAHGSAIN
                     SWFWDFGDGNTSVQQNPEHLYGASGVYSVVLIVENINGCSDTIVQNVTVYALPVASFS
                     YSGTCLSRDINFADNSIQGSGVIDSWTWDFGDGSSFSTNQNPVYQYAVIDSFLVTLIV
                     ADQYGCADTASDSIRVSPLPVSGFMADSVCLGLPTHFTDTSQNFGFTINYWEYDFGDG
                     TTSNLQNPDHIYASAGNYTVQLIVGNISNCFDTVQQMVFVKPAPVAQFISSNICFGET
                     SYFTDSSIPNAAIMNSWSWNFGDGNTSTSQNAQNDYNTAGTFPVELIVANSWNCTDTV
                     LQNTTVYALPEAGFMSSVACLGFPTSFVDTSHAGSVAINGWEWDFGEPGGTSVQQNPT
                     YTYVSGGITYSVTLIVEDQNGCVDTTNMNISLHPQPMVDFSATMACSGLPTSFTDLSV
                     PAAGVMNAWDWNFGDGNTSALQNPQHTYPQSASVQTFNVTLIAYDDNGCPDTVTHTVT
                     VNPQPVAAYSADTACAGEATDFTDLSSATAGAINTWDWDFGDGSGSSALQNPQYTYAS
                     SGVPQFFNATLMIEDLNGCRDTVVHPVLVNPLPVVSFSANAACTGSNTVFSENSFSNG
                     GALTSWVWDFGDGVGASALQNPSYLYSTVLVPTNYNVNLEVTDINGCIYDTTMSVLVN
                     PLPIASFNSISACSGFPSQFTDGSTSTGGNITDWDWDFGDGSGTAILQNPSYQYATTS
                     LVTTYNVVLWVTDQNGCEDSVAMPATIYPSPVAAFVSDSVCSGLASQFTDASTSIGGA
                     ITAWSWDFGDGTGYSSVQSPSYNYAAGLNTASYPVTLIVENINGCFDTLTQNALVWPN
                     PVVDFHSDIACMGSPTHFYDDSYSNGGTLQSWQWDFGDGIGTAAQQYPDYQYDNSGFY
                     DVTLTVTDVNQCVSSAIENIVVDSLPIPDFTYTLSCTQGIVNFTNASQGNGSNIVGYM
                     WDFGDMSNSGQVDPAHYYSATGNYDVTLYVFNDRGCSDSLTIPVNIQPGLEWDFSSTQ
                     ECVGEPTVFNDFAVNPNVPAVAWFWNFGDGSFSTLEDPTHLYLVSGTYNVLLTVTDAM
                     GCAFSVLHNVVVNPRPVADFSATIVTQGNPTTFTDLSSSILGTIISWSWDFDDGNTSA
                     LQNPLHTYALSGTYDVTLIVFNNFGCSDTTTIPITVLPLVEADFVADTVCAKTPMSFS
                     DLSVTGAGTIDYWYWNFGDGYSSALQNPQHTYYNSGVFTVTLIATNTSGISDSTSHQV
                     LVLEAPTAEFLSTSVCQGNATAFMNQSSGTTSNVIGWTWDLGDGDNNFTPSFNHLYDT
                     AGNYNVSLIVVNTIGCRDTVSHQVNVWEIPQVNIAAAPQEGCLPLDVVFTDLTTVGDG
                     TITSWLWQFGDGYTSVSAGGAFHGYTAPGMYGVNLFVTSNHGCSASASFPNYIQAYPN
                     PIASFYYFPTNPSLSDPGVNMVDISVGASSWNWDFSDGESSTEQSPMHEFPNHGNYEI
                     TLIAGNSFGCYDTTSLPIHVAADELLYIPNAITPNGDGRNDWFMPFGAGWSVEHYEMR
                     IYNRWGQLIFVTTDINHAWDGTMQDTGELVQTGVYVWKVWYVDLEGKKQNLIGRVTVT
                     Y"
     gene            complement(20263..20946)
                     /locus_tag="PKY63_03885"
     CDS             complement(20263..20946)
                     /locus_tag="PKY63_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99788.1"
                     /translation="MAARPETDYSFITDGGPIYHETQPGRFVVEPWNAYSSLVFLIPV
                     IYFLIILRGQYRKQGFVVFYTLPLLFTGGIGSTLYHAFRSHHWLMALDVLPMFLLSVG
                     VAFFFMQRLFRNWYLPLIIIIASGLIRGYFFNTLPIQQAINIGYCIVGIVIFVPALLY
                     AWRSQWKAIGHLLAAVLFLALSLFFRLYDDNPVQPMAQGVHWLWHLFSAVGALFAGLY
                     IIRTNKKEE"
     gene            complement(20946..22289)
                     /gene="purB"
                     /locus_tag="PKY63_03890"
     CDS             complement(20946..22289)
                     /gene="purB"
                     /locus_tag="PKY63_03890"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPE99789.1"
                     /translation="MEASLFSVSPVDGRYSDKTRALQQYFSEFALIRYRVLVEIEYFI
                     ALQSLKLPMFKSFTEKKISALRDIVKNFSQSDAQRIKDIEKITNHDVKAVEYFLREKF
                     DELKMEDYKEFIHFGLTSQDVNNTAFPIMLRDALNNAVVPEIESVLKILKTQSKEWNK
                     IPMLARTHGQPASPTTLGKEMAVFAERIENQLTLLKKIPASGKFGGATGNFNAHHAAF
                     PKTDWVKFANNFLKNNLGLIRQQTTTQIEHYDIMSNTFSVLAAINTILIDYSRDIWMY
                     VMMEYFKQKVKKDEVGSSAMPHKVNPIDFENAEGNFGIANSLLRHFSEKLPISRLQRD
                     LTDSTVIRNIGVPLAHMLISLKSLQKGMGKLLINPDKISSDLDLNWAVLAEPIQTILR
                     REKYPDPYNALKNLTRGNAHVTRESLHKFIDTLKVSAAVKKELKTLTPENYTGIH"
     gene            complement(22456..25401)
                     /gene="infB"
                     /locus_tag="PKY63_03895"
     CDS             complement(22456..25401)
                     /gene="infB"
                     /locus_tag="PKY63_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPE99790.1"
                     /translation="MDNNQGVKRLAKVATEFNVGVQTIMEFLNKNGFPVDNPNAKIGQ
                     DAYNALLEEFYLDKTEKQESMKISLSSKKETEEEEVAVPEETKPVKETPAKEVAVKET
                     PAEEKIKPEKEIAPKMEDAPETKVAPKVIGKIDLEGIQAKGKKKSSVKKEKPVEEKPV
                     EEIKKPEPEKPIQEVVQKPAPEEHKVKVELPGVKLVGKIEVENLGKKTKGKKEEKGHH
                     SKKQTVKKEEPVVEKTPEITPEPEIIPPVEEKTPETVAPAEDDRYIKTEFVVLTGPKQ
                     VGKIDVTQFEKKPAAKEETGSDKNRQGRGDKKHRPRIQKKESVSPGSNDIKAPEKSKS
                     KIPKPKRNEPRPVISEEDIQKHIKETLSQLQGAPKSKSSKYRRLKRDAFQQQRMEEME
                     ELEKEQSILKVNEFITVSELASMMNVPAVKVIQSCMSMGLFVSLNQRLDAEAITIVAS
                     EFGFDVSFISLEDEQLPQEEDDDTDETLIPRAPVVTVMGHVDHGKTSLLDNIRKSNII
                     AGESGGITQHIGAYEVTLESGRKIAFLDTPGHEAFTAMRARGAKITDVAIIVIAADDQ
                     VMPQTIEAINHARAAGVPMVFAISKIDKPGANPEKIKEQLANMNLLVEDWGGKYQSQE
                     IDAKHGVNVDALLEKVLLESDMLDLKANPNKLATGSVIESSLDKGRGYTATVLVHAGT
                     LHVGDMVLAGSFYGRVKAMFNERNQPVKEAGPSTPVLILGLNGAPAAGDRLSSHKDEK
                     VAKEIALKRQQLLREQSLRTQKHITLDEIGRRIAIGDFKELNIIVKGDVDGSVEALSD
                     SLLKLSASSVQVNVIHKAIGGITESDVLLATASNAIIIGFQVRPSVAARKLAEVEEID
                     IRTYSVIYDAIEEVKAAMEGMLEPKIEEKVVASVEVREVFKISKLGTIAGCMVLDGKI
                     SRSSKIHLIRNGIVVHTGTLASLKRFKDDVKEVLPGQDCGIGIQNFNDLKAGDIIEAY
                     AEIEVQRKL"
     gene            complement(25515..26750)
                     /gene="nusA"
                     /locus_tag="PKY63_03900"
     CDS             complement(25515..26750)
                     /gene="nusA"
                     /locus_tag="PKY63_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPE99791.1"
                     /translation="METMNLVESFSEFKDSKAIDRPTLMKILVDIFKHILAKKFEVAD
                     NFDVIVNTSTGDLEIWRSRIIVADEDVTNPNMEIAYSEAVKIEPDFEVGEQVVEPFGI
                     TQFDRRDIQSIHQMLKAKVMEFEKENIYQKYKDRIGEIVTGEVNQVWKKELLVLDDED
                     YELVLPRTEMIPSDFYKKGESIRAVVERVEFKNNTPFVILSRISPAFLERLFEMEVPE
                     IFDGLITIRKIVRQPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFSNNLSVFVSRALSPAKLSSLNINEETKTIEVYLKPDQISLAIGKGGQNIKLASKL
                     VDYEIEVFRDVDAEEDDVDLMEFADEIDPWILEELKKIGCDTAKSVLELPREELVKRT
                     DLEEETVDEVIRILQSEFE"
     gene            complement(26770..27216)
                     /gene="rimP"
                     /locus_tag="PKY63_03905"
     CDS             complement(26770..27216)
                     /gene="rimP"
                     /locus_tag="PKY63_03905"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPE99792.1"
                     /translation="MIKENTIIDLARECCGDEKIFPVSVNIRQGNRILVLLDGDTGVT
                     IHDCVRVSRFIENRLDREKEDFELEVSSFGVGNPLVLPRQYINNINRSAKVTLNDQSV
                     ITGTIVAANENSFTIAVKIPPKKKEIQNREIAYTDCLKTQIIVSFK"
     gene            complement(27268..27338)
                     /locus_tag="PKY63_03910"
     tRNA            complement(27268..27338)
                     /locus_tag="PKY63_03910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27304..27306),aa:Gln,seq:ttg)
     gene            27523..30681
                     /locus_tag="PKY63_03915"
     CDS             27523..30681
                     /locus_tag="PKY63_03915"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPE99793.1"
                     /translation="MQNFKRLNTLIGWAVFAIAAVVYTLTAEPTASFWDCGEYIATSF
                     KLQVGHPPGAPTFQLLGRFFSLFAFGDVTQVAFMINMMSVLSSAFTILFLFWTITHFA
                     RKMMGGSVDTASQLWTVIGAGIVGALAYTFSDSFWFSAVEGEVYAMSSMFTALVFWLM
                     LKWEEDADDPRSFRWIILIAFLMGLSIGVHLLNLLTLPALVMIYYYRRYEVSKKGVFY
                     ALLLSVVLLAFMFYLLIPGMVWLAGHFELFFINSIGLPFNSGTIIFFALLIGLIVWGL
                     RWSKRKGRVVINTIVLSFTFLMIGYSTFFVLIIRSNANTPINENSPRDAISLLAYLNR
                     EQYGETPLITGQYFNAPLNQSKEWKDASPIYEKDVEKGEYVMTDPREKSRPTYNKGYT
                     TFLPRMWSSQENHHIATYLEWGGINEKNVYKNVPVTSRGDKIYTQGTPKNPPTFTQNM
                     RFFFTYQVGHMYFRYFFWNFVGRQNDIQGHGNPTEGNWYCGIPAIDKMHLGNQEMVPE
                     HMSKNPGQNAFYFLPLILGLVGLFFHLNKHTKDTLVVTLLFLMTGLAIVVYLNQYPYQ
                     PRERDYAYAGSFYAFAIWIGLGVMAFVQLLKKLFKESILAPVTATLITLLLVPGIMAS
                     EGWDDHDRSSRYTARDFAKNYLASCAPNAILFTNGDNDTFPLWYVQEVEGYRTDVRVV
                     NLSLFNTDWYADQMKRKAYDSDPVPISMEHKQYRTGTRDIIYLLQDEGMGYVNITELF
                     DILHKNPAKLQQKVREYTLDFFPSRNFYLPVDTNKVVQNGIVPASWKNDITDSLNWTI
                     NKNVIQKNTLFCLDILAHFNWDRPIYFAITTGDDAYMGLEDYFSLEGLVYKLVPVKTV
                     NPDGSTGRVNTEVMYDNMMNKFAWGNLTGEGVYLDETNLRMCMNFRSNFARLAMSLLD
                     EGQNEDGSFDAEKKKKAVAVLDRCMEVFPESSVPYNYFVLPVAEAYYRAGEMEKANNI
                     VSRLIDLYDSELAYYFSLDSHRSELLDSEKQQSLYILQRIIVLTEGLKQEVVFKKAEP
                     VFAKYYELFTGVPYNMPRDTTK"
     gene            30684..31424
                     /locus_tag="PKY63_03920"
     CDS             30684..31424
                     /locus_tag="PKY63_03920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE99794.1"
                     /translation="MYVVRPYILKDLNTRNCIWRIPGNEKIIYLTFDDGPIPELTPFV
                     LETLEKYNARATFFCVGDNARKHPQLLREIIAQGHSVGNHTHNHYDGWKKNSEEYEQN
                     IIKGFDYIPGNMFRPPYGHITPWQINRMKDQVYIVLWTVLSYDYDQNTTPARCLRNVL
                     RYTKRGSIVVFHENKKAIPRIKYALPRFIEHYSAQGYRFEALTEPVLQKALHHNWINA
                     SGKFMNKMIGSFNIPFIGSKEAGRKISG"
     gene            31456..32274
                     /gene="deoC"
                     /locus_tag="PKY63_03925"
     CDS             31456..32274
                     /gene="deoC"
                     /locus_tag="PKY63_03925"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPE99795.1"
                     /translation="MTFTNENIQNEISKILTSCAVDQQIAYRRIINHIDLTTLEGNDN
                     DEKVRKLCHSAYEVENAEKQIPSVAAVCVYPVFVKTARESLAGKNINVAAVAGAFPSG
                     QSPLEVRLAEVRYAVEQGADEIDMVISRGRFLAGERDFLREEVSKHKEACGNAHLKVI
                     LETGELLEPNLIYEASMISMEAGADFIKTSTGKIQPAATLEAAYVMLTAIKAYYDKTG
                     KKIGFKPAGGISDPETAVQFWKLVKHINGDEWLNNQLFRYGASRLLNKLVDAVV"
     gene            complement(32592..33419)
                     /locus_tag="PKY63_03930"
     CDS             complement(32592..33419)
                     /locus_tag="PKY63_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE99796.1"
                     /translation="MKIIPIITEVWKIDAGVAFGVIPRSIWSRFYPVGEDNLIPLSNR
                     CLLVETSNRLVLIETGFGNKRDEKYYQYKYISERTSLAQAIQNAGYKPGSVTDVIFTH
                     LHDDHCGGAVNRSQSGESETVCPNAKHWVSKKQWESAMKPNKREAAAYFAENMLPLHE
                     KGLMCFVEKEEEIIPGISVLLRDGHTSGQMLPLINTPARKWLYAADFIPSISHISPVW
                     VASVDVMPILALEEKEIFLHKATEENIGLIFEHDYHHEAATVVKNIKGFEGVPEKIT"
     gene            complement(33433..34788)
                     /gene="radA"
                     /locus_tag="PKY63_03935"
     CDS             complement(33433..34788)
                     /gene="radA"
                     /locus_tag="PKY63_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPE99797.1"
                     /translation="MAKTKTAFVCQSCGSQYPKWNGKCPGCGEWNTLVEEVIAAQPAK
                     TTAFAKFDAPKKLSEIEYSSGKRQSLNNSELDRVLGGGVVEGSVILIGGEPGIGKSTL
                     MLQVAITHPGIKTLYVSGEESLYQIKMRAERLGVKNDNTYLLSETDVQNIIGACEKIQ
                     PDCLVIDSIQTMHTARIESSSGTISQVRECAAELQQYSKKSGVPVFIIGHITKDGSIA
                     GPKVLEHIVDTVLQFEGDRHHGYRILRTLKNRFGSASELGIFEMTAGGLREVSNPSEV
                     FLSDRAIDLPGTAIATTMEGVRPLLLEIQALVSPSPYGNPQRSANGFDQRRMGMLLAV
                     LEKRSGLRMGVNDVFLNVTGGIRVDDPAVDLAVVSALASSYHDMIIDGKICFAAEVGL
                     SGEIRPVNRIEQRISEADKLGFKTIIIAAANNKSLPQKDFSIKIKPVSRLQDVWKIIF
                     S"
     gene            complement(34791..35186)
                     /locus_tag="PKY63_03940"
     CDS             complement(34791..35186)
                     /locus_tag="PKY63_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99798.1"
                     /translation="MKYFNSFVVIVIIIAAAAFSSCTKMKKDKLDEDWRLIRVDQDST
                     ISWFEIWQFQGEMVYMITRPSGGTAIDTLYSAEYTVKSGVSKTTLTMQQCSNTLYNGD
                     WEVLTLNKDMLVLLNRTDGEFVYREFIKE"
     gene            35471..36355
                     /locus_tag="PKY63_03945"
     CDS             35471..36355
                     /locus_tag="PKY63_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE99799.1"
                     /translation="MKISLRSYSELHFVVLLLGFTGILGKLISLPAPHLVVFRVFIAF
                     VTLFLFLKIRRQNIVLTRRQLISCISIGLVVAAHWTTFFYAIKLSNVSVALGLLGVGS
                     LFTALLEPLILRQRFSWLDIITGVFIIIGIYIIFRFESRYVEGIISAIVCYFLSSLFS
                     VLNKKQQEKINPTVLSMYEMLFAFVFALGTLPFLLPNAFTHFWPGSSDLFYLILLGSI
                     CSAYAFTAAIRLMKEMSAFFVVLHINLEPVYAIIIAFFIFGESEHMSLGFYVGAAIIF
                     AAVFIYPFLKKKGMIKKV"
     gene            36417..36899
                     /locus_tag="PKY63_03950"
     CDS             36417..36899
                     /locus_tag="PKY63_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99800.1"
                     /translation="MKTHKFLFLLLLVATPLIAFSQVSWVDYEWKDYSTEFKIPSDFE
                     VTTSSVEKFSATNGAITMTIYPRTEEYLTWEEMKTMLRTWTVDNEVVVSGEIVELDEE
                     KMNGYWGVLLEGTKSDYQVGTMLIVDPDYTEISLYIWVAYDADQVDTVIEMLMSFTPM
                     "
     gene            37034..37735
                     /locus_tag="PKY63_03955"
     CDS             37034..37735
                     /locus_tag="PKY63_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE99801.1"
                     /translation="MVNPKFIIGVIVFIFAFFCGVSMAQELVPTDEMALIIVVVHPEG
                     KPNAVGETVTFKSRKTGKLYRVVTGADAQAKLLLPEGDIYEMSYKDFLTEKDYSMMEV
                     PSEPGMATYEVEVIYQPAKVFKLENVYFDFGKASLRAESYPSLNELAEMLKLKPAMVI
                     EIAGHTDDVGDDASNLTLSQQRAESVRNYLIKKGIAANRIIAKGYGETMPVAGGTSDE
                     ARQKNRRTEVKIISQ"
BASE COUNT         9749 a         7470 c         8638 g        11850 t
ORIGIN      
        1 caaaaaagtt aaaacatata tattgttgca taattggcaa ttgcggagga ctccgaagga
       61 gtcatatatc tgtagaatag tcagaagtaa tactgagtcc gaccaatctc agcgaattga
      121 aaattgatga ttacgatgaa gagcgtgttg ttgatgcaat gatccggtcg gaggcgagcg
      181 gttaccatta tcttcttggc gaagaggatt tcagggcaat tcttggatat atgaaagtgt
      241 gatgctccgc catttctttt tacttttgca aagcaaatat tattcatggc tacccttaaa
      301 aagaaaaagc gttcgttttt cggcagaata tggcggtttt gtctgaaact ggcaatgttg
      361 ttttttatcc tttccattgg aacgaccatc gtttatcgat gggtaaatcc gccggtaact
      421 ccgttgatga ttattcgtct tttcggccag aaaatggatg gaaaagattt gaaattggaa
      481 aaagattggc ttgatattga ttcaatcagc ccaaacatgt ggcatgccgc tattgtgcag
      541 gaggatccga aatttttgta tcataatgga tttgattggg aagcaataaa aattgcacaa
      601 gaatataaca agacacataa aaacaaacgc ggagcaagca ccatttcgca acagaccgct
      661 aaaaatgtct ttttgtggcc cgaccgcaca tggattcgta agggacttga agtgtatttt
      721 acctttttga ttgagacttt ttggagtaaa gaacgtatta tggaggtata tctgaatata
      781 atcgaattcg gtgatggtat ttatggtgtg aacgctgctg ctgacgaata tttcaaaaat
      841 tctgctgaca aactttctaa ggaacaggca gtctcgcttg cggcctgcat tcctgggcct
      901 ctgattatga atgtgaaaaa accaacacag ctttattata ttaaaaaatc ttttatttat
      961 cgatactggg gctatggtcc tgtgaaagaa ctggtgaagc atccatacag tgaatgggaa
     1021 gaaatatatg gaaagaaaag gtgataagaa cgtgtataac tggcttgaga agctggatat
     1081 agcgtatgat tattatgaac atccgccggc tccgactgcc gaaattgcag ctcaatactg
     1141 gaaagacatt gatgcagcgc attgtaaaaa tctgtttttc cgcaatcaca aaggcaatca
     1201 gcattatctg gtcatttttc attatcagca gaatattatg atacgcgatc tcgaacagcg
     1261 tttgaaacag ggtaaactca catttgcttc gcccgaacgc atggatcgat atctgaaaat
     1321 caagccggga agcgtgtctc cgtttggtct tattcacgac aaagatcatc atgtgatcgt
     1381 ttttctcgat gagaatctgc agcaatacga ccgcatcagc tttcatccga atctgaacga
     1441 agcttcgctg gtgattgggt ttggcgattt tataagatac atgaatgcgg tggggaacag
     1501 gtatgagttt ttgagtttat attaatcagt aattgagtgc ggaatgcgaa tgaatgatgc
     1561 cgatataaaa gagataaaga ttgcttgccg tgcaggaata attattccgg ccaatttgtt
     1621 tttgctttct tttcttatag catctgcttt attcattgat aatggcttta ccttaagctt
     1681 ttctctgata ttttcacttc ttacaacttt aattatttcc ggatttgtaa gtttcctgat
     1741 caacagaaat tactatctcg atatcaggga tcaggagaaa gatgagtctt tttcaattgt
     1801 tcaaaagaaa cagcataaaa aagactttga agccggaagc ggtgtcatcg ccaaatttct
     1861 ggcaaaacca atgcgtgaat ttatccgtta cgatattatt gtggacaata ccatttatcg
     1921 tgttgatgaa aagttttaca atgaatgtgc ggagggagac cgcattattc tttattttgc
     1981 gagacacagc agatatcacc ttggatttga attgaagaaa tgaaaaaata tattgacaat
     2041 aatctggggc ttgtttttcc tcagccggta ctgatcggcg gggtcatttt cattgtgatg
     2101 ggtcttcttg tgatgtttga aatctggtac ttcggaatac tcatgatggt cctgggttta
     2161 tatgtggttt tgggtgtgaa cggtgcgcgt attgatgttg aggggcggag attcaaatca
     2221 tattcaaaat ggctctggat caggagtgga caatggaaat ccatggatcc gtatccatat
     2281 gtttctgtac tgggcaaaca gatcggactt acactggcca gccggggaaa ccggacaatg
     2341 gatgttgggg aaataacgta tactgtttgt ttgctatctt cgacacacag gacaaaagtt
     2401 attatccggg cggaggacac gcgccgcgct gctgaaaaat atgccgctta tattgcagaa
     2461 agactgggta aagaggttgt gcagtatacc cctgaaatca gcgcccagac tagggagcgg
     2521 aatatgagga gaagatagtt gttcagagtt gtctttaagg ccgggtgtag tctttaatat
     2581 aaatacgctg ggcgtagtct ccagactacg cccaacagaa aaatttaaac agactacaac
     2641 cggccttaat tatttcatca gctccgaaat gatccggtcg gcaaaaagga ggcccgaacg
     2701 cgtgagtgca agcgactttt cagtgatatg taaatactga aagacattta attttttcac
     2761 ttcttcaatg aattcttccg tgatggattc accgaaaagc tgtttcatct cttccagctg
     2821 aatgccgcaa accatgcgta aacgcgtcag aagaaattca ttgtaaatat ctgttgcact
     2881 caatgtctca ctttcgaaaa ccggcttctg ttcattgatt ccggttatgt attgtggaat
     2941 atccgaaaca ttccacgatc gttttttgcc gtcgaacgaa tgtgccgatg gcccaagacc
     3001 aaggtatgac gcgcttttcc agtaggcact gttatgcttc gattcgaatc cgggtacaca
     3061 aaagttgctg atttcgtatt gtataaatcc gcaggattcg gctttttcaa cagctcgcag
     3121 atacgtttcg gcttgttcgt cttcattgat gtttctaaga acaccttttt tgatatggtt
     3181 ttcgagtata gtgccttttt ccactgtgag cgaatacatg ctcagatgtt tcactccggt
     3241 ttgactgagt tcttcgatgc ttcgaaataa attttcaatg ttggcttcag gaagtccgtg
     3301 aataaggtcg gcgctgatat tgtcgaaacc agcattcacg gcgtctttca gcgcttgccg
     3361 cgaacgattg gccgtgtgcc ggcgtccgag gtattgcagt tctttattgt cgaaagattg
     3421 tgttcctatg ctgagtctgt tcactccctg acttttccag aatacaagtt tatcaggcgt
     3481 aacatcatcg ggattggctt ccagtgtgat ctccgcgtat ttgtcaattg aaaagtgctg
     3541 ctgtatggcc aatgtgattt cgctgaattc ctgctctgtg agcagcgaag gagtgccacc
     3601 accaaaataa attgtttcaa cttttccctt tatttcatct ttcctaagct tcgcttccct
     3661 gcacagtgcc tgcacaaatt caccatgcca tttctttgtg gcaacggaaa agaagtcaca
     3721 gtaaggacat ttcgaatgac agaatggtat gtggatatag aggtgagaca tttgcagtgg
     3781 tcagtattca gttgccaatg gtgagttttt taaatgttat aaaacgttta tcccaaaaat
     3841 tgcagtagct ggttccgttg aaaacgacta ctccgcctcc ggcggagtaa actccctcgt
     3901 cgaataataa tagcccgata ccctgattgc tgagtttgcg aagctcaggg ctacggggaa
     3961 atataacgcg aagcaccctt gaaaaaacat aatcattatt gtcttgtgtc ataagagata
     4021 agtgagtgat aagttatgag ttctgtgtta taagtgatga gttatgaggg atattcatta
     4081 ctggttgctg actgctgaaa actggccact attatgtgcc tttacaaact ttataaactt
     4141 tccactttcc actcaggtac ttagtgctag taagctcnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnngca atgaagtcgc cgagtttggc catgtcgatt ccgtagcagt
     4321 cggggtagcg gatttgtgga gcagaagaag ccaccagaat acttttaggt tcgagccggt
     4381 cgagaatttt gatgatgctt tgacgcaatg tagtgccacg aacaatggag tcgtcaatga
     4441 ccacaagatt atcgatgcca ttgcgaacca gtccatatgt aacatcgtag atatgatcaa
     4501 cgaggtcatc gcgttctttg tcggttgtaa tgaacgtgcg cattttcata tctttcacag
     4561 caatggtttc agcccgagga tgaagtttta atatttcatc gatggcgctg gctgatggat
     4621 ttttcaatgc gagaattttg tcgcgtttta ctttgtcggt gaaatccaga agtgatgcgt
     4681 acatgcccat gaatgctacg attgccgtat tcgggatgta ggaaaaaacc gtattttcaa
     4741 catcgtaatt gatggcttcg agtacttgag gaacaatcag gcggcccagt tttttgcgtt
     4801 ctttataaat gtcggcatcg gttccgcgtg aaaaatatat tcgttcgaag gagcaatatt
     4861 tttcttcttt tggctcaagg atctgttcaa tgcaatatga gccgttgttt tttacaatca
     4921 atgcctggcc aggcaaaagc tctttcacgt tttcgtaatg aatgttgaaa gaagtttgaa
     4981 tgactgggcg ttccgatgtg gcaacaacaa tttcgtcatc ggcataatag aaagccggcc
     5041 ggattcctga tggatcgcgc ataacaaatg aatcaccgtt accgatcata cctgcaagga
     5101 catagccacc atcccagagt ttgcaggatt tacggagtat gttggaaata tttaattcat
     5161 ttgtgatgtg tttggaaatt tcaacccgcg ataaaccttc cgctttatag cgttgataca
     5221 ggaggtcatt ttcttcgtca agataataac caatttgttc catgatagtg actgtatcag
     5281 ccgtttcgac agggtgttga cccatttgaa tgagtttttg aaaaagctca tcagcattgg
     5341 tcatattaaa atttccggca aggataagat ttcgggtttt ccagttattg gggcgaataa
     5401 aaggatgaac gtatttgata ttattttttc cgaatgtacc atagcgcaaa tgacccagaa
     5461 acacttcgcc ggtgaaatca atattttctt tcagatagcc aaggtcgaga tagcgcggtg
     5521 attgtgcggt ttttagttcg tgtaaaggag caaatgcctg gttgaatgca tcgatgatag
     5581 gtgttgatga attgctccgg atgcggttga tgtaaggttt tccgggaggc aaattgagtt
     5641 tgacgcaggc cagtccggca ccgtcctgac cgcggttgtg ctgcttttcc atcagaagcg
     5701 acattttctg aagggcatat accgggttgc catatttggc gagataaaat tccgttggct
     5761 tacggaggcg cagaaatgca attccacact catgcttgat ggcttcgctc attgcagggt
     5821 ttgtatttat tgcaaaaata ctgaaaatgc atgaaataaa attcataaaa catcaaatga
     5881 tattttattt catatagtta taaagattat ttttgtagag tatttgttta tatgtcggaa
     5941 aaccgcagct acaataaccg gaagattttt ttttattttc tgttttcaat aacaggattt
     6001 atttttatta ttaaattgtt tctgattcag gttgttgatg acaagtataa agaatcggcc
     6061 aataacaacg ctttgcgtta tatgtatgag tatccggcac gaggactggt atatgatcgt
     6121 ttcgggaaac tgattgttta taacagtgcc acttttgatt tgatggttat tccacgtgaa
     6181 acagaaagtt ttgatacttt gcgtttgtgc gaaattatag gcataacgaa aaccgaatat
     6241 gacaagcgga tgaaaaaagc catcaatctt tccggttatc atcagccatc tatattggat
     6301 agtgacttgc cggcagacgt ttacagccgt tttcaggaag agagttcatc ttttaagggc
     6361 ttttatattc agagcagaac caagcgtgca tatactttac cagtggctcc gcacacattg
     6421 ggttatattg gtgaggccag cccgatgatt attgaaaaag acccgtatta tcgttcgggc
     6481 gattatattg gaatcaatgg tcttgaaaaa acatatgaaa aggaattgcg cggagagaaa
     6541 gggcttcgtg ttatggtggt tgatgttcat aatacggtgc aggggagcta taaaaatgga
     6601 gaattagaca gggagccgct ttcaggaaaa gatttgtatt cgacacttga tcttgaattg
     6661 cagaattatg ccgaaagtct catggtgaac aagcgggggg gaattgttgc catagagccc
     6721 gctactggcg aagtgctggc gattgtgagt gaaccttcat atgatcccaa tcttttggtt
     6781 ggtcgtacgc gttcaaaaca ttatctggaa cttcagaacg atgtgcaaaa tcatcctttg
     6841 tataatcgcg ccataatgac ccgttatccg ccgggtagta cttttaaggt cgtgaatgga
     6901 ttggcgggat tgcaggaagg catttttgat gatcaaacca tgttttcgtg ttttggcgga
     6961 tatcgcctga gtgagtctca caccattgat tgtcatgccc atccaagtcc tctggctata
     7021 cgtagcgcag tggcctattc atgcaatacc tggttttgct gggcctttaa gaagtttgtt
     7081 gacagcgata aattcacctc ctcgcgcgaa ggatatgagc gttggcgcaa ttatatttat
     7141 aaactgggtt acggacatgc cttgggcatt gatcttccta atgagtttgg gggctatgtg
     7201 ccggaggttg attattatga ccggatgttt ggtcataaaa aatggcatgc caattcaatt
     7261 gtttccgttg ccatcggtca gggcgaaatt ggtgccactc ccttgcagtt ggccaacctt
     7321 acagcgatta ttgccaatcg tggttattat attactccac atgttattcg ggctattggt
     7381 tcggaaaaaa atctgaatac aaaatatatg gagcgccatg aaacaggtat tgataaaagt
     7441 cattatgaga taatcgcaga tgggatggag atggctgtgc tgagcggtac agcaaccgta
     7501 gcgcagatga acggcgttgt tgtttgtgca aaaaccggaa cagctcagga tccgccacga
     7561 aaaaaccatt ctgtttttat tgcgtttgca cccaaagtaa atcctaaaat cgccatagct
     7621 gtgttggtcg aaaacagcgg gtttggtgct acttatgcag ggccaatcgc cagtttgatg
     7681 attgagtatt atctgaacag aaaaatcgaa cgcaaggatc tggaagaaag aattataaac
     7741 actgtattgc ttaatcccaa tcaatgagcc agtttgaaaa taaaacaggg aaaatggatt
     7801 tttgggtgtt gtttatctac atcgccttga tgattattgg ctggctgagt gtttttgcaa
     7861 ccggttacag ggaaggacct ttcagtattt ttgatttttc aatggcttat ggccgtcagt
     7921 cggtatggat tctgacatcg gtattgatgg gactggctat tttgctcatc gatgcgcgct
     7981 tcatcccccg aatgtctttt ggtgtttacg tcggagtact gtttctattg gttcttgttc
     8041 ttgttgttgg aactgttact tcgggaaacc gcgcctggat tgagcttggt gctggtgtga
     8101 aattgcagcc atcggaattt gcaaaatatg gagccgccct gttcttggcc aagtttttaa
     8161 gtattccggg aataaaattc agcaataaaa aaaacagaaa cgccattttt atgattgtgc
     8221 taatcccgat gtttcttgtg tttctgcagg gtgatacggg ttctgccatt gtttttgcct
     8281 cttttttgct tttgtttttc agaatcggaa tgcctggttt ttttattttt cttgttatat
     8341 atatttccat cctttttgtt gcaacattgc tgattaaaca aattgttctg atactgattc
     8401 tggctggaat ttttgcattg cttatttatt tttttcgcga aaagcgcaaa gaaataatac
     8461 gttatctggt tatgtttttt gcttcagcaa tttttattgt cagtgtgaat tatatttttg
     8521 caactgtttt aaaaccgcat caaaaagaca gaatcaatgt gttgatcggc aaagagtatg
     8581 accccaaggg ctctgctttt aatgtcaatc aatcgcttat tgccataggt tcaggtggat
     8641 tttcgggcaa aggttttttg cagggaacac aaacgaaata taattttgtt cccgaacaaa
     8701 ctaccgattt tattttttgc accattggtg aacaatgggg tttttggggt agttctgtgg
     8761 tggttttact gtatgttttt ctgctcattc gtttgattat gatggccgaa aaacaacgat
     8821 ccgattttag tcgttactat atttatgctg ttgccggtat ttttttcctt catttttttg
     8881 tcaatatcgg aatgaccatt gcgctggtgc ctgtaattgg aatcccgctt ccgttcgtga
     8941 gttacggggg ttcgtcgctt tgggctttta ccctcatgct tttcacggta cttaaactca
     9001 atcttcaccg aaattcggtt ctctgatcat gaatccacgt ctgtttattg cttttgaaat
     9061 tgttccttcc gaaataacgc tcagggaatt tgcaaatatc cgacagatat tttcccgcga
     9121 tcggttttca tgggctaagt ctggcctgtt gcatatgacg ttgaaatttt tgggagagac
     9181 tccgacatgc cgcattgaag cagtgaaaga ggccatgaaa gaatccttcg aaaaagaggc
     9241 gtcagtaaaa ttcagactcg aatcgcttgg tgtttttggt tcatcatatg cacctaaagt
     9301 tttatggact catgtatctc ccgaagatat ctgtcgtgtc tggttcacca aactgaaatc
     9361 ttctctgatc aaacgtggtt ttgaatacga tcgtcagaac tttgttccgc atcttacatt
     9421 ggcgcggata aaacaaatcg gtgataagaa tttgcttgct gagacggttg gtaaatacca
     9481 gggattttgt ttcaacgaat ctgatgtgaa ggaaatagtt ctgtatgaaa gtgtgttgaa
     9541 accaacaggt gcagaatata ttaaacattt ctctgtttct cttcattaat ttgttttttt
     9601 gtaagtttga acaatgaaac tcagagcgga aaatattgta aaacgttata agtcgcgcac
     9661 agtcgtaaaa ggcgtttctt tcgaagtcaa tcagggtgag attgttggat tgcttggtcc
     9721 caatggtgca ggcaagacga cttcgtttta tatggttgtg ggtctcataa aacctaatga
     9781 aggaaaggtt tttcttgatt cggaagagat gaccgatttg ccgatgtatc gcagagcgca
     9841 gaaaggtatt ggttatctgg ctcaggaagc cagtgtgttt cgtaaactca gtgttgaaga
     9901 taatatcagg tctgttcttg agtttacaaa gttcacaaaa caggagcaag cggataaggt
     9961 tgaaacactc ctggatgaat ttggactgca gaaaatccgt aaaagcaaag gaattcaact
    10021 tagtggtggt gagcgtcgtc gtactgaaat tgccagagca ctggcggtca atccgcattt
    10081 cattctgctt gatgaaccct ttgctggtgt agatcctatt gcggttgagg atattcagga
    10141 aattgtatca aaacttcgcg ataagaatat tggcatattg attaccgatc ataatgtgca
    10201 tgaaactttg tcaatcaccg accgcgccta tcttttgttt gaaggatcaa ttattaaagc
    10261 cggctcggcc gaagaacttg ccgacgatga atatgtgcgc aaagtgtatc ttggtaaaaa
    10321 ttttgaatta cgcaggtctg ttaaatagcg cgatatatgc gtatttgtgt tttatttgtc
    10381 agtttatttt taatcatttc ggcttatccc caaacaacga agtattcggt tgttttgcat
    10441 gggggagctg gtaattttga ctcaaaaaca ttttcacctg cgcagaaaaa caattataat
    10501 gatttttttg aaagtattct tgcaatggct gatagcatgg ctattgccgg agattcggcg
    10561 ataaaaatcg ttacggctgt tattgtgaaa atggaaaaca gtcctatgtt caatgccgga
    10621 cgtggtgctg tgttgactga ggacggcatt gctgaactcg atgcttcggt catggatggc
    10681 actacgctca aagccggtgc tgttgcgggt gtacgcataa taaagaatcc aattctggcg
    10741 gcacaaaaag tgatgacaaa ttctcagcat gtgtttctgg ctggacgtgg tgctgaacaa
    10801 tttgcttcac tgcaggggct cgaaatggtg gacagcagct attttgcaac agatgaaatg
    10861 aaaaagaaat ttgaaaaaat caaagctgat aagaacggaa ccgtgggctg tgtggtgcga
    10921 gacatttacg gaaatatttg tgcaggaacc agtaccggtg gtatgatgat gaaaaaatgg
    10981 ggccgcatcg gcgacagtcc ggtgattggt ggcggaacct atgccgatag ccgttgggcg
    11041 ggtatttcat gcacagggca cggtgaattt ttcatccgct acgcagtggc ctatgatgtt
    11101 gttgcccgca tgaaatatgg ccggcaaaca ctgaaagaag ccatgcatgc ggtgttgttc
    11161 gatgtactgc ttccggccgg aggcactggc ggaatgattg gaattgatgg ttccgggaat
    11221 attgttgttg atttcaacac cactggtatg tttcgtgctt cgaccaccca gagcggggtg
    11281 catgagattg aatattagat tatataagaa ataagagcac aaaagcaatc agcaaaagtt
    11341 ccagtaaaaa aagtatggag catagcaata ctgctattat agcgattgct ttgtttttgt
    11401 atttgtttgg attgttttct gttttgttta ttgcgataac gccaaaaatg attgaaatca
    11461 gcgaaagtgg aaaagacagc cccgtgatta aaaatcctgc aatggctgct gctgccaggc
    11521 cggcgcttac gatggtggcc caatgtgttt ttggtggatc gtctgcgtag cttgcagaaa
    11581 cggatttgcg attatgacca aatacattct taaagtcact tgctttaatt tcagttgaaa
    11641 catccaacgg attattacag caataaatca tttcgttgat ttgtgattct gcaatttttt
    11701 ttgttgaatc ggatgtattg attctactga ttgtagaaat ggctgaaatt tttatttcat
    11761 cttccaatgg taagtcagtg caaatatctt catgatttat tataggtttt tctgtctgat
    11821 gttttttgtt gcggtctgaa atcagggagt acccgtttct gtacactctt ttatcaagct
    11881 gacatgaaga cagcaatagt aagaagctga agaaaatcag tatatgtgtt ttcatcgcaa
    11941 tttaacagta aaagttttct tgtctgaaaa ttgatatgat tatcatgaaa accttttgct
    12001 ttcaaaaata gacaattttt tcaactgctt gtaaatgatt gcctgacctg atattttttt
    12061 attttgtaaa gcgggttgtt aaccaaatgc taccttcaga attaatatgt attgtggatt
    12121 attttgttca ttcatgccaa gtatggatta aatttgcata ttctgttttg tcaaaaagca
    12181 gtcagagtag tctaatgtct atgtgccggt ttcgaaaatt tttactgttg gtttttttca
    12241 atgcagccgt tttgcacgga gtagcgcaat gtccggtaaa tgcatggttt acatggacgc
    12301 gtgtgtgcaa caacagcatg gtgcatttta cagatagcag ttttttgacc ggaacgggac
    12361 aaattgtgga ttggcaatgg gattttggtg atgcttcgcc tggaagcagc aatccaaatc
    12421 cgaatcataa ttttccgggt attggaacct acactgtaac actgattgtt acagattcct
    12481 ctggttgcac cgatgtggca actattcctg taagcgttga tccgcttcct gtggctgaat
    12541 tcactttcgg tccactgaat gtttgttcgg gaaatgttgc ttcctttact tttaccggca
    12601 gtggcaatgg tttaacgtat gaatggaatt ttggtgatgg tcagacttct tcgcagcaga
    12661 atccttcgca tgttttcaca tcttttggtt gtctctcgca aaattttaat gtttcactaa
    12721 cggtaactga tgaaaacggt tgttcttcaa cgatatctca tcaggtttta gtcttgcagc
    12781 agcccaatgt gttttatttt gaacccaacg gttttgtatt ttgtcatacc gatacgtctt
    12841 cgatttcaga taccattcat gtttataatt tttcaccgga tattggatgc attgattcct
    12901 acagcattga ctggggcgat gggactccgg cttctacatc accacccgaa ttgttcgatg
    12961 gcatgcatcc gctcaatcat gtttacacca atatcgggta ttatattgtt actataacag
    13021 ctgtcggata caatggttgt cagacggttt ttaccgacac ggctacggta gaaagcaatc
    13081 cggtggcttc actgatcggt cctcctgtgg gaagcaatgt gggctgtgca ccgctcaatg
    13141 tgtgtgtggt gaacatgagt cagaatgtgt cagcatcaac ggtgatgacg gtggattggg
    13201 gcgatggcgt gattgaaaca ttgctcccgt catcagttgg tcagaccatt tgtcataatt
    13261 acctgatttc gggttgtgtg gctggttcta tgaccaatta tgctgttatt cttacagctc
    13321 agaatgtgtg tgatttttct cagtcatcct ggtcacctgt gcgtgtgtat gaaccacccg
    13381 aagctgtttt taatatagtg gatgattctg tttgtgttgg cgaaaacgca acttttatca
    13441 atgtttcgga acccaatttg tgcgctgcag cgtcggcaac ctggtacacc tgggattttg
    13501 gcgatggaac aacgtcgggt cctactctgg tgcctgccgg atccagtccg cagcaaacca
    13561 ttgcacatgc ttatggcgat accggtgttt atgttgttac tcttaccgcc aacaacagct
    13621 cggtaaatgg ttgcggaatg acttcgtttc agggtttggt atatgttagt cagacctatg
    13681 cccagttttc ttatgacact gtttgctttg gagacccgac tcattttgtc gattcaagct
    13741 gggcgttgag ttcatccatt gccacctgga actggaattt tggtgacggt ggtaccagta
    13801 ctgtgcagaa tccaaattac acctatacca attggggcga tttcaatgca acgttgacag
    13861 ttacttccga cgccggttgt acagcggtca tgacgcatgt ggtgcatgtc gatacactgc
    13921 cttttgtaca gtttgcatgg gatacggttt gcgtgggcga tccgactact ttcaccaatc
    13981 tgtcttatgg ccgcggagcg ccgattataa gctatttctg gaacttcggc gatggaaata
    14041 cttctacgtt tgaagatcca actcatattt atggatcagc agggagcttt atcgtaacgc
    14101 ttacagtcac agatgccaac ggctgtgtca ataccatttt gaattctgtt cctgtaagtc
    14161 cgccacccat tgccgatttt gatacggata cggcatgtgt gggttccgct tcggttttca
    14221 ccgatcagtc caccgctccg tttggtacca ttgtcagctg gaactgggat ttcggcgatg
    14281 gaagcggaac atcggccata cagaatccaa cttatatgta tgcagacacc ggcaattaca
    14341 atgttacact gattgttgaa accaatatcg gctgcgttga taccatcacg cttccggtgt
    14401 atgtagcgcc cattcccctg gctgatttca ccttcaatat tgtgtgtgca ggcaatacga
    14461 caacattcac tgatgcctct caaagctacg gttggttcat cacttcgtgg caatggaatt
    14521 tcggcgacgg caatacagca acaacacaga atccgacgca tatctatgcg gaccctggag
    14581 tgtacacggt ttcgctcatt gtggcaaatc agtttggttg cagcgatacg attgttcatt
    14641 ccgttcctgt tgattcaatt cctactgcgg cttttgttgc caacaatgcc tgtttgtatc
    14701 aaaccgttgg ttttaccgat ctatcaattt cgcatggaag taatatcgtt ggctgggaat
    14761 gggattttgg tgatggcact acttctgtgc tgcaaaatcc aacgcatatt tatgcagcat
    14821 ctggcgttta ttctgttaca cttattgtga cttctgcaat cggttgtatg gactcggctt
    14881 ctttgccggt gtcggtttat ccgttgccag aggccggatt cactatggtt tcggcatgcg
    14941 aaggtgaccc cgtaagcttt actgatacct cgcttgcaaa tggtggttcc atcgtttcat
    15001 ggcaatggga ttttggcgat ggcagtggta cttcggcatt tcagaatccg caatattttt
    15061 atacggatac cggaatatat tatgtaacgc tgattgtatt caatagtcat ggctgtagtg
    15121 ataccgtgat aaatccggtg tattatctgc cccggccgac tgcagaattt gtgcatgaca
    15181 ccagctgcgc tgggcagccc attactttta ccgatgtgtc agccggcaac ggaagcacca
    15241 ttgtcagctg gacatgggac ttcggtgacg gcaatacttc tgcattgcag aatcctgtgc
    15301 atccatacgc aacatcggga tcgtttttgg tgacgctgat tgtcagcagt aattcaggat
    15361 gcgccgatac cgttcagcac atggtcgtgg cggattcgct tcccgagccg ttgtttacag
    15421 cgccgtcagt atgtttcaac gatacaactt atttcacgga tatgagtatt gcccacggat
    15481 ctgccattaa cagctggttc tgggattttg gcgatggtaa tacttcggtg caacaaaatc
    15541 cggaacattt gtatggcgca tctggagtgt attctgttgt gctgattgtt gaaaatatca
    15601 acggctgttc ggataccatt gtccagaatg tgactgttta tgcgcttcct gtcgcttcgt
    15661 tttcttattc cggaacttgt ctgagtcgtg atataaactt tgcagataat tcaattcagg
    15721 ggagtggagt tattgattcc tggacctggg attttggcga tggcagcagt ttttcaacaa
    15781 atcagaatcc ggtttatcag tatgcagtca ttgatagttt tctggtaact cttattgttg
    15841 ctgatcaata tggttgtgct gatacggcat cagacagcat acgcgtaagc cctttgccag
    15901 tctcgggttt tatggctgat tcggtttgtc ttggtttgcc aacacatttt accgacactt
    15961 ctcaaaattt tggttttacc atcaattatt gggaatatga ttttggtgac ggaacaacat
    16021 ccaatcttca aaaccctgat catatttatg cttcagcagg taattatact gttcagctga
    16081 ttgtcggcaa tatcagtaac tgttttgata cagttcagca gatggtgttt gtaaaacctg
    16141 ctccggtggc tcagttcatt tcctcaaata tttgcttcgg agaaacgagt tattttacgg
    16201 attccagcat tcccaatgca gcaataatga acagctggtc atggaatttc ggcgatggca
    16261 atacctcaac aagtcaaaat gctcaaaatg attacaacac agcgggcacg tttccggttg
    16321 agcttattgt tgcaaattca tggaattgta cggatacggt tttgcagaat accactgtct
    16381 atgcgctgcc tgaggcggga tttatgtcga gtgttgcctg ccttggtttt ccgacttcgt
    16441 ttgttgacac atcgcatgca ggaagtgttg caatcaatgg atgggaatgg gattttggcg
    16501 aacccggcgg aacatccgtg cagcagaacc cgacttatac ttacgttagc ggaggcatca
    16561 cctattcggt tactttgatt gttgaagacc agaatgggtg tgttgataca acgaatatga
    16621 atatttcgtt acatccgcag ccaatggttg atttttctgc aacaatggcc tgttcaggtc
    16681 ttccaaccag ctttacagac ctttcggttc ctgctgccgg tgtcatgaat gcatgggact
    16741 ggaatttcgg agatggtaat acttcggctt tgcaaaatcc gcagcacacc tatccgcaat
    16801 ccgcttcagt ccagacattc aatgttacat tgattgcata tgatgacaac ggatgtcctg
    16861 atacggtcac acatacagtt actgtaaatc cccaaccggt agcagcgtac agtgccgata
    16921 ccgcctgtgc cggcgaagca actgatttta ccgatctttc atcagctaca gctggagcaa
    16981 tcaacacctg ggattgggat ttcggtgatg gcagtggatc atctgcattg caaaacccgc
    17041 aatataccta cgcttcatcg ggtgtaccac agtttttcaa cgccacgctg atgattgaag
    17101 atttgaatgg atgccgcgat acagtagttc atccggtgct tgtgaatccg ttgcctgttg
    17161 taagtttttc agccaatgcg gcttgcacgg gttcaaatac tgttttcagt gaaaattcgt
    17221 ttagtaacgg aggcgcgctg acttcatggg tttgggattt cggagatggt gtgggagcgt
    17281 ctgcattgca gaatccatca tacttgtatt cgacggttct tgtgccaact aattataatg
    17341 taaatcttga agtaacagat atcaatgggt gtatttatga taccacgatg tctgtacttg
    17401 taaatccgtt accaattgct tcgttcaatt caatatcggc ctgttctggc ttcccaagcc
    17461 agtttacaga tggctccacc agcactggag gaaatataac agattgggac tgggatttcg
    17521 gagatggcag cggaacggcc atccttcaga acccatcgta tcaatatgca acaacatcat
    17581 tagtgacgac atacaatgtt gttttatggg taactgatca aaatggctgt gaagacagtg
    17641 ttgccatgcc ggctacaatt tatccatcgc ctgttgcagc ttttgtttca gatagtgttt
    17701 gttcgggatt ggcttcgcag tttaccgatg catcaacatc cattggtggc gctattacgg
    17761 cgtggagctg ggattttggt gatggcacag gatattcttc tgttcaaagt ccttcatata
    17821 actatgctgc aggattgaat acggcctcat atccggtgac gctgattgtt gaaaatataa
    17881 atggttgttt cgacacactc actcaaaatg cgcttgtgtg gccaaatccg gtggttgatt
    17941 ttcattcgga tattgcctgc atgggatcgc cgactcactt ctatgatgat tcttattcca
    18001 atggtggcac gcttcagagt tggcaatggg attttggcga tggcattggt actgctgcac
    18061 aacaatatcc ggattatcaa tacgacaatt cagggtttta tgatgttaca cttacggtca
    18121 ccgatgttaa tcagtgtgtt tcatcagcta ttgaaaacat tgttgtggat tcgttgccta
    18181 ttcccgattt tacatatacg ctctcatgca ctcaggggat tgtgaatttc accaatgcct
    18241 cgcaaggcaa tggcagtaat attgtcggtt acatgtggga ttttggcgat atgagcaatt
    18301 cggggcaggt cgatccggct cattattatt ccgctaccgg gaactatgat gttacattat
    18361 atgtattcaa tgatcgtgga tgttcggatt cattgacgat tccggttaac attcaaccag
    18421 gtttggaatg ggatttttcg tcaacgcagg aatgtgttgg agaaccaaca gtattcaatg
    18481 attttgcagt caatccgaat gtacctgctg ttgcgtggtt ctggaatttt ggcgacggct
    18541 ctttttcaac acttgaagac ccgacgcatc tgtaccttgt ttcgggaact tacaatgttt
    18601 tacttacagt gactgatgca atgggatgtg ctttttcagt gttgcacaat gttgttgtta
    18661 accccagacc tgtagctgat tttagtgcta cgatcgtaac gcagggaaac cctaccacat
    18721 ttacagattt atcatcatca attcttggaa ccatcatttc atggagctgg gactttgatg
    18781 acggaaatac atcagcactt cagaatcctc ttcacaccta tgctttatct ggtacctatg
    18841 atgtgactct gattgtattc aataatttcg ggtgctctga tacaacgaca attccgataa
    18901 cagtcttgcc tttggtcgaa gcggattttg ttgcagatac cgtgtgtgca aaaacaccga
    18961 tgtccttttc ggatctttct gtcacaggcg caggaaccat tgattactgg tactggaatt
    19021 ttggcgacgg ctatagttca gcgttgcaga atcctcagca tacttattac aattcaggag
    19081 tgtttactgt tacgctgatt gcaacgaata catcgggaat ttcagattcc acttcgcacc
    19141 aggtgttggt gctagaagct ccgacggcag aatttttatc cacatcggtt tgtcagggaa
    19201 atgctaccgc attcatgaat cagagcagtg gtacaaccag caatgtaatt ggctggacat
    19261 gggatctggg cgatggcgat aataacttca ctccaagttt caatcatctt tacgatacag
    19321 cgggcaacta taatgtttca ttgattgttg tgaatacgat tggttgccgc gatacagtaa
    19381 gtcatcaggt gaacgtgtgg gaaattccgc aggtgaacat tgctgcagca ccacaggaag
    19441 gatgtctgcc actggatgtt gttttcacgg atctgacaac agtcggcgat ggaactatta
    19501 catcgtggct ttggcagttt ggtgatggct atacttctgt ttctgctggt ggtgcttttc
    19561 acggatatac tgctcccgga atgtatggtg taaatctttt tgtaaccagt aatcatgggt
    19621 gttctgcgtc ggcttcattt ccaaattaca ttcaggcata tccgaatccg atagcctcgt
    19681 tttattattt tccgaccaat ccgagtctga gcgatccggg tgtaaacatg gtggacatta
    19741 gtgttggagc cagcagctgg aactgggatt ttagtgacgg tgaaagttcg acagaacaaa
    19801 gtccgatgca tgaatttccc aatcacggaa attatgaaat tacattgatt gccggaaaca
    19861 gctttggatg ctatgatacg acgtcattgc cgatacatgt tgccgctgat gaattgttgt
    19921 atattccgaa tgccattaca cccaacggtg atggtcgtaa tgattggttt atgccttttg
    19981 gcgcaggatg gtcggttgag cattatgaaa tgagaattta caaccgctgg ggacaactga
    20041 tatttgttac taccgacata aatcatgcgt gggacggtac gatgcaggac accggagagc
    20101 ttgttcagac gggcgtttat gtgtggaaag tgtggtatgt agatctcgaa gggaaaaagc
    20161 aaaatctgat cggaagagtc acagtgacgt attgatagct gtcagaaagc cagtgatctg
    20221 ttatgataag gccagaattt ggcactgaac gatgattgaa cgctattcct cttttttatt
    20281 tgtgcggata atatacaatc ctgcgaaaag cgctcccaca gctgagaaca gatgccacag
    20341 ccaatgaact ccctgcgcca ttggttgcac aggattgtca tcgtacagac ggaaaaaaag
    20401 tgacagcgcc agaaacagaa cagccgccag cagatgtccg atggctttcc attggctgcg
    20461 ccaggcgtag agcagtgccg ggacaaatat tacgattcct actatacaat aaccgatgtt
    20521 gatggcttgc tgaatgggga gtgtgttgaa aaaatatccg cggattaacc ctgatgcaat
    20581 gatgataatc agcggcagat accagttgcg gaacagtcgc tgcataaaga aaaatgcaac
    20641 accgactgac aataaaaaca taggtaaaac atcgagcgcc atcagccagt ggtggctccg
    20701 gaaagcgtga taaagcgtac ttccgatgcc gcctgtgaaa agaagcggca gtgtataaaa
    20761 caccacaaat ccttgtttgc ggtattgacc tcgcagaatg atcagaaaat aaatgaccgg
    20821 aatcagaaat accagcgatg aatacgcatt ccatggttcc acaacaaagc gacccggctg
    20881 ggtttcatga tatatgggtc cgccgtcggt gataaaagaa tagtctgttt ccggtcgggc
    20941 tgccatcagt gaatgcctgt ataattttca ggagtcaacg ttttcaattc tttttttacc
    21001 gcggcactca ccttgagcgt gtcgatgaat ttatgcagac tttcgcgcgt tacatgcgcg
    21061 ttcccgcgtg taagattttt cagtgcattg tatggatccg ggtatttttc gcgacgaaga
    21121 atggtttgaa ttggctcagc cagtacagcc cagttaagat cgagatcgct gctgatttta
    21181 tcggggttga taagtaattt tcccattccc ttctgcagcg attttagtga tatgagcatg
    21241 tgtgcgagtg gaacaccaat atttcttata acagttgaat ccgtcaggtc gcgctgcaaa
    21301 cgtgatatcg gaagcttttc ggaaaaatga cgcaataagg aattggctat accgaagttc
    21361 ccttctgcgt tttcaaagtc aatcggatta actttgtgtg gcatggccga tgagccgact
    21421 tcatcttttt ttactttctg tttgaaatat tccatcatga catacatcca gatgtcgcgc
    21481 gaataatcaa tcagaatggt attgatggcg gcaagaacac tgaatgtatt ggacataatg
    21541 tcgtagtgtt caatctgtgt tgttgtttgc tgtcggataa gacccagatt gtttttcaga
    21601 aagttgttgg cgaattttac ccaatctgtt tttggaaaag ctgcatggtg agcgttgaag
    21661 tttccggtgg cgccaccaaa ttttccactg gcgggaattt ttttgagaag tgtaagttga
    21721 ttttcgattc gctctgcaaa aacagccatt tcttttccga gggtggtggg cgaagccggc
    21781 tgtccgtgcg tgcgtgcaag catcggaatt ttattccatt cttttgattg agttttgaga
    21841 attttaagaa cggattcgat ttcgggtaca acggcattgt ttaaggcatc acgcagcatt
    21901 atcggaaacg cggtgttgtt aacgtcctga gaggtaaggc cgaaatgaat gaattctttg
    21961 taatcttcca ttttaagctc atcgaatttt tcacgaagga aatattcaac tgcttttaca
    22021 tcgtgattgg tgattttttc aatatctttg atgcgttgag catcgctctg gctgaagttc
    22081 ttaacgatgt cgcgaagtgc agaaattttc ttttctgtga aacttttaaa catcggtaat
    22141 ttgagcgatt gaagcgcaat aaaatactca atttcgacca aaacacggta acggattaag
    22201 gcaaattcgg agaaatactg ctgaagggct cgcgttttat cgctgtagcg gccgtcaacg
    22261 ggcgacactg aaaacaggga tgcttccatg cggatggttt tttgcaaaaa tacaatttaa
    22321 tgcgctgatt tgaaaatttg aaaaagtgct aatttgaaaa agcactttct actaagcaca
    22381 acgagtcagc ttagaatcaa aactgtttgt tgattattcc gtgccttgcg gacgggactt
    22441 cgctggctgc tgtttttaca gtttgcgctg gacttcgatt tcggcatagg cctcaatgat
    22501 gtcgccagct ttcagatcgt tgaaattttg aattccgata ccgcaatcct gtccaggcaa
    22561 aacttctttg acgtcatctt taaaacgttt caaagaagcc agggtgccgg tatgaacaac
    22621 aataccattt ctgatgaggt gtattttgct gctgcgtgaa attttgccat caagcaccat
    22681 gcagccggca atggttccca gttttgagat tttgaagact tcacgtactt cgacactggc
    22741 aacgactttt tcttcaattt ttggttcaag cataccttcc atggcagctt ttacttcttc
    22801 aatggcatcg tagatgacag aataggttcg gatgtcgatt tcttcaactt cggctaattt
    22861 acgtgcagca acgctgggtc gaacctggaa tccgataata atggcgtttg atgctgttgc
    22921 cagcaacacg tcagattcgg taattccacc aatggccttg tgaattacat tgacttgaac
    22981 actgcttgca ctgagcttga gcaatgaatc ggacaaagct tcaacagagc cgtcaacgtc
    23041 gcccttaaca ataatattga gttctttgaa atcaccaatg gcaatacgac gcccgatttc
    23101 atcgagagta atatgtttct gtgttctgag tgattgttcg cgcaaaagct gctgacgttt
    23161 gagtgcaatt tcttttgcaa ctttttcgtc tttatggctg gatagtctgt cgcccgcagc
    23221 aggagcgccg ttgaggccaa gaatgagtac cggagtgcta gggccggctt cttttactgg
    23281 ttgattcctt tcgttgaaca tggcttttac gcggccataa aaactaccag caagtaccat
    23341 gtcgccaaca tgaagtgtac cggcatgaac aagaactgta gctgtatatc cacgtccctt
    23401 gtccagtgac gattcaataa cagaacccgt tgcgagtttg ttgggatttg ccttgagatc
    23461 gagcatgtct gattcgagca atactttttc aagcaaagca tcgacattga ccccatgttt
    23521 tgcatcaatt tcctgacttt ggtatttgcc tccccaatct tcgaccagaa gattcatatt
    23581 ggcaagttgt tccttgattt tttcaggatt tgcaccgggt ttatcaattt tgctgatggc
    23641 aaagaccatt ggaactccgg ctgcccgtgc gtggttgata gcttcgatgg tttgcggcat
    23701 cacctggtcg tcggcagcaa taacaataat ggctacatct gtgattttgg ctccgcgggc
    23761 acgcatggcc gtaaatgcct catggccggg agtgtcgagg aatgctattt ttctgccgct
    23821 ttctagcgtt acttcataag caccaatgtg ctgtgtaatt ccaccggatt caccggcaat
    23881 gatgttgctt ttgcggatat tgtcgagtaa ggatgttttt ccatgatcaa cgtgacccat
    23941 cactgtaacc actggcgcgc gtggaatcaa ggtttcatca gtgtcatcat cttcttcttg
    24001 tggcaattgt tcatcttcga gtgagataaa actcacatca aatccgaatt cagaagcaac
    24061 aatcgtgatt gcctcggcat cgagacgttg gttgagtgac acaaaaagac ccatactcat
    24121 gcagctttga atgactttaa ctgcaggtac attcatcatg gaggcaagtt cgcttactgt
    24181 gatgaattca tttactttaa ggatgctttg ttctttttcc agctcttcca tttcctccat
    24241 gcgttgctgt tggaatgcat cgcgttttaa acggcggtat ttggatgatt ttgattttgg
    24301 cgcaccctga agttgtgaaa gagtttcttt gatgtgtttt tggatatctt cttcagaaat
    24361 aaccggacgc ggttcattgc gttttggttt cggaattttg cttttgcttt tttcaggtgc
    24421 ttttatgtcg tttgaacctg gtgaaacaga ttcttttttc tgaatgcgtg gccgatgttt
    24481 tttatcacct cgtccctgac ggtttttgtc ggatccggtt tcttctttgg cagctggttt
    24541 tttctcaaac tgggttacat cgatttttcc aacctgtttt ggaccagtca ggacaacaaa
    24601 ttctgttttg atgtaacgat catcttcggc aggagcaact gtttccggtg ttttttcttc
    24661 aaccggagga attatctcag gttcaggagt gatttccgga gttttttcaa caacaggttc
    24721 ttcttttttt actgtttgtt tttttgaatg atgtcctttt tcttcttttt tccctttggt
    24781 ttttttcccc aggttttcaa cttcaatttt accgactagt ttaacgccag gcagttcaac
    24841 tttgacttta tgttcttccg gagcaggttt ttgaacaact tcctgaatcg gtttttcggg
    24901 ctctggtttt ttaatttctt caaccggttt ttcttcaacc ggtttttctt tctttaccga
    24961 agatttcttt tttccttttg cctgaattcc ttcaaggtca atttttccaa taacctttgg
    25021 cgctaccttt gtttcgggag catcttccat tttgggagcg atttcttttt cgggttttat
    25081 tttttcttcg gcaggtgttt cttttacagc aacttctttt gcaggcgttt ctttaacggg
    25141 ttttgtttct tcaggaacag caacttcttc ttcttccgtt tctttttttg aagaaagtga
    25201 aatcttcatg ctttcctgtt tttctgtctt atccagataa aattcttcga gtaatgcatt
    25261 gtatgcatcc tgtccgattt tcgcattggg attgtcaaca ggaaaaccat tcttattcag
    25321 aaattccatg atggtttgta ccccaacatt gaattctgtt gcgacttttg ctaatctttt
    25381 tactccctgg ttattgtcca tatcttgttt ttcggttcct gaaaacgctg caaaattaca
    25441 aaattttatt cactggctta aataacaggg gtttctgaac aatgatgatt ttcaaaaagc
    25501 ccctgtttag ttgattattc aaattcactt tgcaaaatcc tgatgacttc atcaacggtt
    25561 tcttcctcaa ggtcggtacg cttaacaagc tcttcgcgcg gaagttcaag tacactcttt
    25621 gcggtatcgc aaccaatctt tttgagttct tcgagaatcc atggatctat ttcatctgca
    25681 aattccatca ggtcaacatc gtcctcttcg gcgtccacat cacggaatac ttcgatttcg
    25741 taatcaacaa gtttagacgc cagcttgatg ttttgaccgc ctttgccaat ggcaagtgag
    25801 atctggtctg gcttgagata tacctcgatg gtttttgttt cttcgttgat gttgagtgaa
    25861 gatagtttgg cgggactcaa tgcacggctg acaaaaacag acaggttgtt tgagaaatta
    25921 atcacgtcaa tgttttcgtt gcgcagttcg cgaacaattc catgaatacg tgatcccttc
    25981 attccaacac aagctcctac aggatcaata cggtcgtcgt aggattcaac ggcaactttg
    26041 gctctttcgc ccggctgacg aacaattttg cggatggtga tgagtccatc gaaaatttca
    26101 ggcacttcca tttcgaacag acgttcgagg aaggccggag aaatgcgaga cagaataacg
    26161 aaaggggtgt tgtttttgaa ttcaacgcgt tcaacaacag cacgaatgga ttcacctttt
    26221 ttgtaaaaat cggaagggat catttctgtt ctgggcagca cgagttcgta atcttcatca
    26281 tcaagtacta gcaattcttt tttccatacc tgattgactt cacctgtaac gatttcacca
    26341 atgcggtctt tgtatttctg gtaaatattt tccttttcaa attccatcac ctttgctttc
    26401 aacatctgat gaatgctttg aatgtcgcga cggtcgaatt gtgtgattcc aaatggttca
    26461 actacctgtt cgccaacctc aaagtcaggt tctattttca ctgcttcgct gtaagcaatt
    26521 tccatatttg gattggttac atcttcgtcg gcaacgataa ttcggctacg ccatatttca
    26581 aggtctccgg tcgatgtatt gacaataaca tcgaaattgt cggccacttc gaatttcttg
    26641 gcaaggatat gtttgaaaat atccaccagg attttcatca acgttggccg gtcaatggct
    26701 ttggagtctt taaattcgct gaaggattca accagattca ttgtttccat aacacaaagg
    26761 gtgtttttat tatttaaacg atacaattat ttgcgttttc aggcaatcgg tatatgcgat
    26821 ttccctgttt tgaatttctt tctttttggg cggaattttc acggcaatcg taaaggaatt
    26881 ttcattggcg gcgacaatgg taccggtgat tacggactga tcgttcaatg ttacttttgc
    26941 actgcggtta atattgttga tgtactgacg aggcaaaacg agcggattgc caacgccaaa
    27001 agatgatact tcgagttcga aatcttcttt ttcacggtca agccggtttt caatgaaacg
    27061 acttacccga acgcaatcgt gaatagtaac gccggtatcg ccatcaagca ataccagaat
    27121 gcgattgccc tgccggatgt ttaccgacac cgggaaaatc ttttcatcgc cgcagcattc
    27181 gcgggccaga tcaattatgg tgttttcttt tatcattaat tccgcttaat aatcgagggg
    27241 acaattgtcc cctcgcaaag tctcctgttg tccgaccagg actcgaacct ggaccagcag
    27301 aaccaaaatc tgctgtgctg ccattacacc atcggacaat tttgaaacag ggtgcaaaag
    27361 tacgaattat ttcaatatca gcaccatttt tattgatatt tgactcaatc cggattcaca
    27421 ttgcgttgaa aatggcttag aatggtactt tcaaatgata aacatctgca tttgaaataa
    27481 aattgtaatt tcgcagttta ttcacggaac aacacattga atatgcaaaa cttcaagcga
    27541 ctaaacactt tgattggatg ggctgtattt gccattgcag ccgtcgttta cactcttacc
    27601 gctgaaccta cagccagttt ttgggattgt ggtgaatata ttgccacctc ttttaaactt
    27661 caggtgggtc acccccccgg agcacctact tttcagctgc ttggtcgttt cttctccctg
    27721 tttgcttttg gcgatgtaac tcaggttgct ttcatgataa acatgatgtc tgtgctttcc
    27781 agcgccttta ctattttgtt cctgttctgg actataacgc attttgcgcg caagatgatg
    27841 ggtggaagcg ttgatactgc ctctcaatta tggactgtta tcggtgcagg tattgtagga
    27901 gctcttgctt atactttttc tgactcattc tggttttcag cggttgaagg cgaggtttat
    27961 gccatgtctt cgatgtttac agcgcttgtt ttctggctta tgctaaaatg ggaagaagat
    28021 gccgatgatc cgcgttcatt tcgttggatt attttgattg ctttccttat gggcctgtct
    28081 attggcgttc accttttgaa cctgctgact ttgccggctc ttgtaatgat ttattattac
    28141 cgtcgttacg aagtttcaaa aaaaggagtg ttctacgctt tgttgctttc tgttgttctg
    28201 ctcgctttta tgttttattt gctcattccg ggtatggttt ggctggcagg acattttgaa
    28261 ctgttcttta ttaacagtat tggccttccg ttcaattcag gaaccatcat attttttgcc
    28321 ctgctcatcg gacttattgt ctggggtctt cgttggtcca aacgtaaggg gcgggtggtg
    28381 ataaacacca ttgtgctttc ttttacattt ctgatgattg gttattcaac attctttgtt
    28441 ctcattattc gttccaatgc aaataccccc atcaacgaaa attcgccgcg tgatgccatt
    28501 agtctgctgg cttatctgaa tcgagaacaa tatggagaaa cacctcttat tacaggtcag
    28561 tattttaatg ctccgctcaa tcaatccaaa gaatggaaag atgcctcacc tatttatgaa
    28621 aaagatgttg aaaaaggtga atatgtaatg acagatccgc gcgaaaaatc gcgaccgacg
    28681 tataataaag gttatactac atttttgccc cgtatgtgga gcagtcagga aaatcatcat
    28741 attgccacct atctggagtg gggtggaatt aatgaaaaga atgtgtataa aaatgttcct
    28801 gtcacttcgc gtggtgataa aatatacaca cagggtacac caaagaaccc accgacgttt
    28861 acccagaata tgcgtttctt tttcacctat caggtgggac atatgtattt caggtatttt
    28921 ttctggaatt ttgtcggtcg tcagaatgac attcaggggc atggaaatcc aaccgaagga
    28981 aactggtatt gtggaattcc ggccatcgat aaaatgcatc tcggaaatca ggaaatggtt
    29041 ccggaacaca tgagtaagaa tccaggccaa aacgcatttt attttcttcc attaatactt
    29101 ggtctggttg gtttgttttt tcatttgaat aaacacacca aagatacact ggtagtaaca
    29161 ttattatttt tgatgaccgg cctggccatt gttgtgtatc tcaaccagta tccctatcag
    29221 ccgcgtgaac gtgactacgc ctatgccggt agtttttatg cctttgccat ctggatcgga
    29281 cttggtgtta tggcttttgt acaattgttg aagaaacttt ttaaggaaag cattctggct
    29341 cctgttacag caacacttat tacgcttctt cttgttcccg gcattatggc cagtgagggt
    29401 tgggatgatc atgaccgcag cagtcgttac accgcaaggg attttgccaa aaactatctg
    29461 gcttcctgtg cgcccaacgc catattattt accaatggag ataacgacac atttccttta
    29521 tggtatgttc aggaagttga gggttaccgc accgatgtgc gtgtagttaa tctctcactt
    29581 ttcaataccg actggtatgc cgatcaaatg aagcgtaaag cgtatgattc agatcctgtg
    29641 ccgatttcca tggagcacaa acaataccgg acaggaaccc gcgacattat ttatcttttg
    29701 caggatgagg gaatggggta tgtaaacatc actgaacttt ttgatattct gcataagaat
    29761 cctgcgaaac tgcagcagaa agttcgggaa tatacgctgg attttttccc gtcacgtaat
    29821 ttctatttgc cggttgacac caataaagtt gttcagaatg gcattgttcc ggcatcgtgg
    29881 aaaaatgata ttaccgatag tctgaactgg acaatcaaca aaaatgtaat tcagaaaaac
    29941 actttgttct gtcttgatat tttagctcat tttaactggg accggccgat ttattttgcc
    30001 ataacaacgg gtgatgatgc ctatatgggt ctcgaagatt atttttctct tgaaggcctt
    30061 gtttacaagc ttgtccctgt aaagactgtc aatccggatg gtagtaccgg ccgtgtgaat
    30121 acagaggtaa tgtatgataa catgatgaat aaatttgcct ggggcaatct gaccggtgaa
    30181 ggagtttatc tcgacgagac caatttgcgt atgtgcatga atttcagaag taattttgca
    30241 cgattggcaa tgtcattgct tgatgaaggg cagaacgaag atggttcatt cgatgcagaa
    30301 aagaagaaaa aagccgttgc tgttcttgat cgttgtatgg aagtctttcc tgaatcatcg
    30361 gtgccgtata actattttgt acttccggtt gctgaagcat attatcgtgc cggagaaatg
    30421 gaaaaagcaa acaatatcgt ttcaagattg attgatttgt acgattctga gcttgcatat
    30481 tatttttctc tcgacagcca ccgaagcgaa ttgcttgatt cagaaaaaca gcagtcactg
    30541 tatattttgc agcgcattat tgtgttgacc gaagggttga aacaggaggt tgtctttaaa
    30601 aaggcagaac ctgtttttgc aaagtattat gaactcttta ccggtgttcc ttataatatg
    30661 ccaagggata ccacgaaata ggcatgtatg tcgtaaggcc ttatatactc aaggatctca
    30721 atacccggaa ttgcatctgg cgcattccgg gtaatgagaa aattatttac ctgacttttg
    30781 atgatggccc aattccagag cttacaccct ttgttttgga aacgcttgaa aagtataatg
    30841 cccgtgcaac atttttctgt gttggagata atgcccgaaa acatccgcaa ttgctgcgcg
    30901 aaattattgc tcaggggcat tccgtaggga atcataccca taaccattac gatggatgga
    30961 aaaaaaactc ggaggagtat gaacaaaata tcataaaagg atttgattat attcccggaa
    31021 acatgttcag acctccatac gggcatatta caccctggca gataaaccgc atgaaagatc
    31081 aggtttatat tgttttgtgg accgttctca gctatgatta cgatcagaat acaacaccag
    31141 cacgttgttt gcgtaatgtt ttgcgttata ccaaacgtgg atctattgtg gtttttcacg
    31201 aaaataaaaa agccattccc cgtatcaaat atgcgctgcc tcgttttatt gagcattact
    31261 cagcacaggg ttatcgcttc gaagctttga cggagccggt gttgcagaaa gcgcttcatc
    31321 acaactggat caatgcttca gggaaattta tgaataaaat gattggctca ttcaatattc
    31381 cgtttatcgg atcaaaggaa gccggacgaa aaatatcagg ttaaacttcc tgttcaccat
    31441 ttaaaacaga ttagcatgac atttacaaac gaaaacattc agaatgaaat cagtaaaata
    31501 ctgacttcat gtgctgttga tcagcagata gcgtacagac gtataatcaa tcatatcgat
    31561 ctgacgacgc tggagggaaa cgataatgat gagaaagtgc ggaaactctg tcattctgct
    31621 tatgaagtgg agaatgcaga aaaacaaatt ccgtcggttg ctgcggtttg cgtttatcct
    31681 gtttttgtta agaccgctcg ggaatcattg gctggaaaaa acatcaatgt tgcagctgta
    31741 gccggtgctt ttccatcggg tcaaagcccg cttgaggtgc ggcttgcaga agttcgttac
    31801 gccgtggaac aaggcgccga tgaaattgat atggtgattt cgcggggccg ctttctggcc
    31861 ggcgaacgcg atttcctgcg cgaagaagtt tcgaaacaca aagaagcatg cggaaatgcg
    31921 catttgaaag tgattctgga aacaggagaa ttgctcgaac caaacctgat ttacgaagca
    31981 tctatgattt cgatggaggc cggcgccgat tttattaaaa catcgaccgg gaaaatacaa
    32041 cccgctgcca cactcgaagc agcttatgtt atgctgactg caatcaaggc ctattacgat
    32101 aaaacaggaa agaaaatcgg cttcaaacct gccggcggca tcagcgatcc cgaaaccgct
    32161 gttcaattct ggaagctggt gaaacacatc aacggcgacg aatggctgaa caatcagttg
    32221 ttccgctacg gtgcaagccg cctgctgaat aagctggtgg atgccgttgt ttaaataggg
    32281 cctgcggaaa aaacacaggg caatcagatc agtatttctt cattgccggt gggctgctat
    32341 caattgctgt agaaaaatga cgggaaaatg taaaggagcg gcgtcttttg ataatgcttg
    32401 ttaacatgcg atccgccagc cggcggatcg tgtatgcgtt attggttttc tacagatatg
    32461 agatgcctgc ctgtttgtca tgtaaacctc tggcatcagc caccaaaata aaatacgata
    32521 atatgttatt ataggatcat ccgataattc actttggtat ttatttcaat cgaaattttc
    32581 ttactgcaga cttaagtgat tttttcaggg acaccctcaa accctttgat gttttttact
    32641 actgttgctg cttcatggtg gtaatcgtgt tcgaaaatca aaccgatgtt ttcttctgtg
    32701 gctttgtgca gaaaaatttc tttttcttcg agtgccagaa tcggcattac atcgactgat
    32761 gcgacccata caggagaaat gtgcgaaatg gaaggaatga aatcggcggc gtagagccat
    32821 ttccgcgcag gggtgtttat gagtggcagc atttgtccgg acgtgtgacc gtcgcgcagc
    32881 aacaccgaaa tgccgggaat aatttcttct tctttttcaa caaaacacat tagtcctttt
    32941 tcgtgcagcg gcagcatgtt ttcggcaaaa taggcggcgg cttcgcgttt attgggcttc
    33001 atggccgatt cccattgttt tttgcttacc cagtgttttg cattcggaca cacagtttct
    33061 gattcaccgc tctgagagcg gtttacagct ccaccgcaat gatcgtcgtg caggtgagtg
    33121 aaaatgacat ccgtcactga accgggcttg tatcctgcgt tttggatagc ctgtgcaagc
    33181 gaagtgcgct ctgaaatata tttatactga taatattttt catctctttt atttccgaat
    33241 ccggtttcaa tcagcacaag ccggttcgaa gtctcaacca gcagacaacg attgctaagc
    33301 ggaatgagat tgtcttcgcc tacaggataa aagcggctcc agattgagcg ggggattaca
    33361 ccgaaagcaa cgcctgcatc tattttccag acttcggtaa ttattggaat gattttcatg
    33421 ctgcaaaatt aatcaactga aaataatttt ccacacatcc tgcaaacgtg atacaggctt
    33481 gatttttatt gaaaaatctt tttgtggaag cgatttgttg ttggcagcgg caatgattat
    33541 ggttttgaat ccgagtttgt cggcttctga aatgcgttgt tctatgcggt ttacaggtct
    33601 tatttcgccg gaaagtccaa cttctgccgc gaagcaaatc ttgccgtcaa tgatcatgtc
    33661 gtgatacgag gaggcaagtg cactcacaac ggccagatcg accgctggat catcgacacg
    33721 gatgcctccg gtgacgttga gaaaaacatc attcacaccc atgcggaggc cgctgcgttt
    33781 ttcgagaacg gccagcagca tgcccatgcg gcgctgatcg aagccattgg cgctgcgttg
    33841 cggatttccg taggggctcg ggctcaccaa tgcctgtatt tcgagcaata atggcctgac
    33901 tccttccatg gttgttgcaa tggctgttcc cggcaaatcg atggcgcggt ctgaaagaaa
    33961 aacctccgaa ggattggaaa cttcgcggag gccaccggcg gtcatttcaa atataccaag
    34021 ctcagaggca gatccgaagc ggtttttcag cgtccgaaga attctgtatc catgatgccg
    34081 gtcgccttcg aactgcagaa cagtatcgac gatatgttcc aatactttgg gacctgcaat
    34141 gcttccgtct tttgtaatgt gaccgatgat gaaaacaggc acgccgcttt ttttggaata
    34201 ctgttgcagt tctgctgcgc attcacgaac ctgactgatg gtgccggaag acgattcaat
    34261 gcgggctgtg tgcatggttt gaattgaatc aatgaccagg cagtcgggtt gtattttttc
    34321 gcaggcacca ataatattct ggacatcggt ttcggaaagc agataggtgt tgtcgttttt
    34381 cactcccaga cgctctgcac gcattttgat ctggtacaga ctttcttcac cggaaacata
    34441 cagggttttg atgccgggat gcgtaatggc cacctgtagc attagtgttg atttgccgat
    34501 gcccggctcg ccaccaataa gaatgacaga tccttcgaca actccgccgc caagcacgcg
    34561 atcgagttcg gaattgttga gtgattggcg tttacctgat gaatactcga tttcagaaag
    34621 ttttttcgga gcgtcgaatt ttgcaaatgc agttgtttta gcaggctgag ctgcaatgac
    34681 ctcttcaacc agagtattcc attcgccaca gcccggacat ttgccgttcc attttggata
    34741 ttgagaaccg caactctgac aaacaaaggc ggttttggtt tttgccattt ttattcttta
    34801 ataaactccc tgtaaacaaa ctccccgtct gttctgttaa gcagaaccag catgtccttg
    34861 ttcagggtga gaacttccca gtcgccgttg taaagtgtgt ttgagcattg ctgcatggta
    34921 agtgtggttt ttgaaacacc tgactttaca gtatattcag ctgaatataa agtatcgatt
    34981 gctgttccgc ctgatgggcg ggtaatcatg tacaccattt cgccctgaaa ctgccagatt
    35041 tcaaaccagg aaatcgtaga gtcctgatca acccttatta gtcgccagtc ttcatcaagt
    35101 ttgtcttttt tcatttttgt acatgagctg aaagctgcag cggcaatgat gatgacaatt
    35161 actacgaaac tgttaaaata tttcattgag gtcatggttt tatatgcaaa catacaaaaa
    35221 taatttattt cgtaaaacag aatattaaag gtcagcaagc tgaaaatgag gtcatggaca
    35281 aggttaaggt cgaggccgag gttaagcacc ttcggtgacc agcgagcagt gagcggcgat
    35341 tggtgaacct tgctgaataa gcaaagaagc tatttctaca ttcgagcatc atttcagatt
    35401 tcgatgttcg gtgttcactg ttcagtgttc catgttctta cgtatctttg tcccgttttt
    35461 aaataaatac atgaagattt ccctccggtc ttattccgaa ttgcattttg ttgttctttt
    35521 gcttggcttc accggaatcc tgggtaagct catttcatta cctgcgccgc atctggttgt
    35581 tttcagggtt tttattgctt ttgttacctt atttttattt ctaaaaatcc gtcgtcagaa
    35641 tattgttttg acgcggcgtc agttgatttc gtgtatttcg atcggattgg ttgtggctgc
    35701 ccactggact acctttttct atgcaattaa gctttccaat gtgtctgttg ctcttggatt
    35761 gcttggggtt ggttcattgt tcacagcatt gcttgaaccg cttattttgc gacagcggtt
    35821 ttcgtggctt gatattatca ccggtgtttt tattattata ggcatttata ttatttttcg
    35881 tttcgagagc cgttatgtcg agggtattat ttcggctatt gtttgttatt tcctttcatc
    35941 gcttttcagt gtgcttaata aaaagcagca ggaaaaaatc aatcccacgg tgttgagcat
    36001 gtacgaaatg ctttttgcat ttgtatttgc gctgggtact ctgccatttc ttttgccaaa
    36061 tgcctttact catttctggc cgggttcttc tgatttgttt tatctgattt tgctcggaag
    36121 catttgttcg gcttatgcgt ttactgctgc cataagacta atgaaggaaa tgagcgcttt
    36181 ttttgttgtt ttgcatatta atcttgaacc tgtttatgcc attataattg cgttttttat
    36241 tttcggcgaa tcggagcata tgtctttagg tttttatgtt ggtgctgcca ttatttttgc
    36301 agctgtgttt atttatccgt ttttgaaaaa gaaagggatg ataaaaaaag tataaatatt
    36361 tttattgact tttaaactga taggtgaatt tcatatctta gtacaaaaat caccttatga
    36421 aaactcataa atttcttttt ttgctcttgc ttgttgcaac accgcttatt gctttttctc
    36481 aggtatcctg ggtggactat gaatggaagg attacagtac ggaattcaaa attccgtctg
    36541 attttgaagt tactacttct tctgttgaaa aattcagtgc taccaatggg gcaataacaa
    36601 tgactattta tccaagaacc gaagaatatc ttacctggga ggaaatgaaa acaatgctca
    36661 gaacctggac agtggataat gaagttgttg tgtctggcga aattgtcgag cttgatgaag
    36721 aaaaaatgaa cggctattgg ggcgttttgc tcgaaggaac caagtctgat taccaggttg
    36781 gcactatgct gattgttgac cccgattaca cagaaatcag tctctacatt tgggttgcct
    36841 acgatgccga tcaggtcgat acggtaattg aaatgctgat gtcttttact cccatgtgac
    36901 agaaagagtg ttttctgtat ttatcacttt tcttggataa tcgttttatc agatcatgtt
    36961 gatttgtttt ttttgtaaca cataacaaat cgggtgataa aaacgtttat ctttatatca
    37021 aaatttattt gttatggtga atccgaagtt tattattggg gtcattgttt ttatttttgc
    37081 gtttttttgt ggtgtttcca tggctcagga acttgtgcct accgatgaaa tggcgctgat
    37141 aattgtagtg gttcatccgg aaggtaagcc caatgctgtt ggtgaaacgg ttacttttaa
    37201 aagtcggaaa accggcaaat tgtatagagt tgtaacgggt gctgatgcgc aggccaaatt
    37261 gcttttgccg gaaggtgaca tttatgaaat gtcgtataag gattttttga ctgaaaaaga
    37321 ttacagcatg atggaagtgc cttccgaacc gggaatggct acttatgaag ttgaagtgat
    37381 atatcagccg gcaaaggtgt ttaaacttga aaatgtttat tttgattttg gcaaagcttc
    37441 tttgcgcgcc gaatcatatc cgtcgttgaa tgaacttgct gaaatgctta aactgaagcc
    37501 agccatggtg attgaaattg ctggtcatac tgacgatgtg ggcgacgatg catctaatct
    37561 taccttgtcg cagcaaagag ccgaatcggt cagaaattat ctcattaaaa aaggtattgc
    37621 cgccaaccgt attattgcca aaggttatgg cgaaaccatg cctgttgctg gtggtaccag
    37681 cgacgaagcc aggcagaaaa accgcagaac cgaagtgaag attatttcgc agtagcgaaa
    37741 gaaatacgaa actctttcag cggtgagtag tgaatggtga gcagcgatta tgtggaaatg
    37801 tgcgcct
//
LOCUS       DAOPKE010000018        37728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1156_length_37728_cov_11.840761, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000018 DAOPKE010000000
VERSION     DAOPKE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37728
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_37728_cov_11.840761"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKY63_03960"
     CDS             <1..246
                     /locus_tag="PKY63_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99802.1"
                     /translation="TLTWLFPCRRQLNLNLNLSLAVLPASGSSTSTSTLTWLFPCRRQ
                     LNLNLNLILAVSPATGSSTYALTLALFLALHRFNNFI"
     gene            complement(214..570)
                     /locus_tag="PKY63_03965"
     CDS             complement(214..570)
                     /locus_tag="PKY63_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99803.1"
                     /translation="MYTSFTQMPVWKTAFDLSTEIFELTSNLPKSEDYGLTSQIRRSA
                     NSVGANISEGFGRVTGNDKAYFYVVSRGSAYETKHHLRYGCAVGYFDKEVVEKLIKQY
                     QDLINSLNKIIKTMKS"
     gene            complement(1143..1643)
                     /locus_tag="PKY63_03970"
     CDS             complement(1143..1643)
                     /locus_tag="PKY63_03970"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPE99804.1"
                     /translation="MNINDKDNLRQWHVAYTAPRAEKKVSDRLSEQGINHYLPLKKTK
                     KQWSDRRKWVEEPLFPSYIFVQVDRKEYFDAINTDGMVCYIRFEGKAAMVREQTMSDI
                     RRIAEAGLMAEMVDEMPRPGTRYSINAGPLKGISGRVVRLKGKTHFVLEVEELGKFLV
                     LPFSAS"
     gene            complement(1983..2888)
                     /gene="gldA"
                     /locus_tag="PKY63_03975"
     CDS             complement(1983..2888)
                     /gene="gldA"
                     /locus_tag="PKY63_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HPE99805.1"
                     /translation="MSIRVDQVTKVYGEQKALNNVSLEIGKGEIVGLLGPNGAGKSTL
                     MKILTCYIPPTSGQAFVNEMNCADESLNVRRMIGYLPENNPLYYDMYVREFLTFIAGI
                     HNLKDKKQRVDEMIEITGLKPEMHKRIGALSKGYKQRVGLAQALIHNPEVLIMDEPTS
                     GLDPNQIVEIRDLIRKLGSEKTILLSTHIMQEVEAICNRVVIIDKGKIVADGKTGDLS
                     SMFAAAELFLVEFDRKVTREALLGVKGVKNANTKGDNMWAVEADQSIDVRKEIFDFAV
                     RNQCKVLSLTKEKHSIEELFQQLTK"
     gene            complement(3009..4271)
                     /locus_tag="PKY63_03980"
     CDS             complement(3009..4271)
                     /locus_tag="PKY63_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPE99806.1"
                     /translation="MNRVIFFIFFAGMSFFSMAQEFELSGDFRFRPEYRNGYKQLPDS
                     NTEAAGFVAQRNRIGLNYTSEKFETKLILQESRVWGDELFKSNKATVGMLEAWVRMKI
                     ADSLKIKMGRHQLVFGNQRLFSGNNWSQTSQEHDAVMLEWKSGTKQVYFVQAFNQRAE
                     NIFGTDYLNNVSEIKDNYKTLTVLRYEQKIKNFEYSVMGFADGFQDLNGKALYVRSTQ
                     GVELKYTLNKFIFSGMGYYQTGHNISGTELAAWFAASEAEYKTDKWSVKLGGEMMSGN
                     DVTTDKYEQFIPQYASNHAFAGHMDYFTNFATSTGGAGFIDTYLKGHFKFSKIHSLAL
                     DYHYFATQQSTITGGANIDQFLAHEIDLTWKAALAKDMVLSVGYSVFSGSTTLELLRG
                     GDHTKLAHWAYVMLEFKPKFLKYVFEKK"
     gene            complement(4599..5363)
                     /locus_tag="PKY63_03985"
     CDS             complement(4599..5363)
                     /locus_tag="PKY63_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPE99807.1"
                     /translation="MTNNQPIIDIRNLSVHAGETKLLNNISVKIPRETITVIMGPSGC
                     GKTTLLRSMNRLTDLNGLKVKGQIHFGGQEILSLRGYHVSAMRQRMGLLSQRPYPLPL
                     SIFENIAYAVRLKGISRKTEVRSQVEHYLKLACLWDEVKDRLNQSPSTLSIGQQQRLC
                     LARGLAVDPEVILADEPTSALDPISTQAIEQSFMQLKSDYTIILVSHILRQARRIADH
                     VIFMYMGEMIESGDSQTLFSNPENEMTKKYLEGVFY"
     gene            complement(5367..6212)
                     /gene="pstA"
                     /locus_tag="PKY63_03990"
     CDS             complement(5367..6212)
                     /gene="pstA"
                     /locus_tag="PKY63_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPE99808.1"
                     /translation="MKHKYFEEFVMKTLMISATLIIAIALGSILYGIVVRGIPYLNWS
                     MISSLPKGGYYFGKEGGILNAIIGSLYLALGATFLALILSVPVALYMNVFLQRNSKAV
                     SFFRFFLDVLWGIPSIVYGAFGFTIMVALGIRTSLLGGIVVVGIFVAPIMIRAMDEVL
                     QRVPNGIREAAYSMGCTKTETAFRFFLRKAAPGIVTAILLSFGRAIGDAAVVLLTTGY
                     TDYIPTSLDQQVATLPLAIFFQLSSPVEEVQGRAYASALILTIIILFISISARIIGRV
                     YKKTI"
     gene            complement(6202..7107)
                     /gene="pstC"
                     /locus_tag="PKY63_03995"
     CDS             complement(6202..7107)
                     /gene="pstC"
                     /locus_tag="PKY63_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPE99809.1"
                     /translation="MIATRRMRDYAGFAWMVISKIIIIIVPLIMLYVIIDKSLPLLQE
                     NGFVNILSGDDWKPAAGSFGFRPFIIGSLYVSVLAILISAPVCILAAIYLSEYAHRRI
                     LGFMQPVIDILAGIPSVIYGVWGILFIVPFVRNVIAPVAGIESTGYSVLAGSLVLAVM
                     IIPFVLNILLELFATVPLELRESAYSMGTTKWEMVKVTVLKKTGAGIISAIGLGWARA
                     IGETIAVMMVVGNTIAIPKNIVSPGYPLPALIANNYGEMMSIPKYDSALMFAALILLV
                     ITVVFNILMRLIIRRFTTKTADNEA"
     gene            complement(7107..8057)
                     /locus_tag="PKY63_04000"
     CDS             complement(7107..8057)
                     /locus_tag="PKY63_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE99810.1"
                     /translation="MKTYSVIFLVFAIVVTGCGNKKKEEKSDQLRGNISISGAFALYP
                     MAQKWAEEFMKENPDVRIDISAGGAGKGMTDVLNGMVDMAMFSRSVSPEEESNGAWKI
                     AVAKDVVLPTINAKNPVYTELVTRGLTKEEFEKIYMTQEINNWNQLKLKGSGAINLFT
                     RSDACGAAEMWAKFMGGEQADLKGVGVFGDPGIADAVKNDIKGVGFNNIIYAYDFKTG
                     KVQDGIGIIPIDMNGNGKIDKEENLYSDMKLLTGAIREGKFPEPPARPLYFICKGKPD
                     NQLVVAFLRFILTKGQQWVEESGYVQLPDAMIEAELKKLD"
     gene            8301..8870
                     /locus_tag="PKY63_04005"
     CDS             8301..8870
                     /locus_tag="PKY63_04005"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPE99811.1"
                     /translation="MEFSWYEFVVLPLLIFLARIADQSVGTLRLIFLARGIKFWVPIL
                     GFCEVIIWLLAVGQILNDLTNWVAIIAYGAGFAAGNLIGMWIDEKLSIGTLIVRVIPK
                     FDTTGLIADLSSRDFGVTVSDASGARGPVKIVMSIIKRKDLTTFVEIVQKHNPNAFYT
                     VEDVKAVSEGVFRASKRRSIFSFNSRIGK"
     gene            complement(9334..10431)
                     /locus_tag="PKY63_04010"
     CDS             complement(9334..10431)
                     /locus_tag="PKY63_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE99812.1"
                     /translation="MSNLKLLVISNYSGLNSSRPEAELMLGLKKAGVNITIMTPGTAE
                     YTPVFEAAGIRVIDFQPHKKFDRSEIDFIRKELIVGAYHVVHLFNGKAIINGLKAARG
                     LNVKVVLYRGYCGNIHWWDPLAYFKFLHPRADAVWCIAPAVADLINRNKIFGKKNAFC
                     ITKGHHPDWYKNVDAVSYDEFKIPSGSFVVTMVANARPMKGLKYFIKATYEIKQDLSL
                     YILLIGNGLYSDEIRKLVEASPMKERIIFTGYRSDSMGLVKNSDVFVLSSIYGEAICK
                     SVIEAMSLGVAPVITDIPGNSGMVIDGESGLVIPRKNPSAIARAIEYMYEYPEERTKM
                     AEAARQRMQSEYHIDSTIEKMLAFYKKIAAK"
     gene            complement(10570..11997)
                     /gene="dnaA"
                     /locus_tag="PKY63_04015"
     CDS             complement(10570..11997)
                     /gene="dnaA"
                     /locus_tag="PKY63_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPE99813.1"
                     /translation="MAKDIKTIWTNCLKVIKDNIDDHSFSTWFAPIKPLKLDGNVLTI
                     QVPSSFFYEWIEEHYIDLLKKTIRKELGQDGKLEYNILVDATQNGISVNYPSSDRNAL
                     KNPSVDIPIDINRGTSKEIPNPFIIPGLRKIKVNSQLVESLNFDNFVEGDCNRLARSA
                     GLAIAKNPGKTSFNPLFIFSDTGLGKTHLAHAIGIEVKKNFPDKTVLYVQTSEFLNQF
                     IDSVKDDTTNDFVHFYQMIDVLIMDDIHNLANKLKTQDVFFQLFNTLHQKGNQIILTS
                     DKPPVELEDIEPRLLSRFKWGLAADLQVPDIETRIAIIRKKLHKDGITIPDDVVEYLA
                     YSISTNIRELEGAMISLVAHSSFNRKPITIDLAKTMIDQYVRNSKREISIDYIQKMVC
                     DYFNISVEQMHSSSRKREIVQARQLAMYFAKTMTKSSLASIGVDIGGKDHATVLHAVR
                     TVKNLIDTDKHFRVYVEEIEKKIKL"
     gene            complement(12152..13096)
                     /locus_tag="PKY63_04020"
     CDS             complement(12152..13096)
                     /locus_tag="PKY63_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE99814.1"
                     /translation="MGGSGMSNRRDFLKTMLAAGASLALPSVLQAGSEKKTKKIILLQ
                     TNDMHSQIDPLPETHHRFPGMGGMAARAALISKIRGEGHPVLLFDSGDVFQGTPYYNM
                     YGGEVEMKLMSEMNYSAATIGNHEFDKGLDGLADAMEYATFPFISSNYIFKHPRLSKS
                     VKRYKIFTFGKIKIGVFGLGVYPYGLISKITFSDTEYRDPVVTAAEMAYFLKKEEKCS
                     IIICLSHLGYKADEDGQTADYDLARQSKNIDFILGGHSHTLLSKPVSVFNSDNKQVLI
                     TQNAYAGVRMSRIDCYFSSAGDLIFVDAYTNNIFKKQV"
     gene            complement(13074..13850)
                     /locus_tag="PKY63_04025"
     CDS             complement(13074..13850)
                     /locus_tag="PKY63_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPE99815.1"
                     /translation="MKTNKRILILAAFMWSVVLLSSVLISCKTAVLSSSTAVSLIVDD
                     SLGVDSSIVETIAPYRKQLDSSMKEVIGYSETMLEKGMPESLLGNFLSDLLIERVKST
                     SHDSMRSLPVMSLLNNGGLRSSLPRGEIMIENVFQLMPFDNEIVIIELKGSVLMKLFD
                     IIAQKGGMPVGGLRLVLNATSWTNAEIEGQVVDAEKNYILITSDYLATGGDGLSFLSD
                     NVRYVQTGLLMRDVFISGIRDLTAQKKTINPKTDGRIRYE"
     gene            14238..15131
                     /locus_tag="PKY63_04030"
     CDS             14238..15131
                     /locus_tag="PKY63_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99816.1"
                     /translation="MKLLHTLSFVFLVFSLLFANNTGLPDIERKELKETNQENPENVK
                     SKSVFVAFHDKLVSVPVGYSVNSQVLFGARFILNGETFLAQDYSTIEYFISPIKGGKI
                     VGTYFPKSEGFNEFSSVYGKFIISADGKNGSDLYVVEFSNGSFSKPERLSLNTEYNEF
                     NGIFSNDGEKIYFTSDRPGGFGGLDIYEIELIGKNKWSQPKNLGPSINTAADEQCPFM
                     LKDGMTLYFSSNGHVSNGDFDIYIVTQGDDGEWGDPDKLGEPINSDKDDLYYRMSPDE
                     TRAVYYTFGESGEGIYELIFN"
     gene            complement(15525..16091)
                     /locus_tag="PKY63_04035"
     CDS             complement(15525..16091)
                     /locus_tag="PKY63_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPE99817.1"
                     /translation="MEAIDKKYVDQARELWPELDWIKDAELKEKTTLTWALALQKSVL
                     TPEDLRTIPFTLLAGPDLKVSFMDHKRAVVHIARDAGLKCGEFFRTDLPVNMDVLIAG
                     AILADVGKLLEYEMKDGKSVQGMYGKYLRHPFSGVSLAEQCGVPADVCHIIATHAGEG
                     NMVKRTTEAFIVHHADFMTFEPFKDRLK"
     gene            complement(16099..17298)
                     /locus_tag="PKY63_04040"
     CDS             complement(16099..17298)
                     /locus_tag="PKY63_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPE99818.1"
                     /translation="MAKRTIVAMPGDGIGQVVLEESIRVLDAAGFDANYVTGDIGWEF
                     WCKEGNPLPDRTIKLLEEHKIGLFGAITSKPKDKAAAELAPELRDKGLVYYSPIVTMR
                     QKFGLDICMRPCQTLKGNPLNFVRRNATGGVDEPVVDAMIFRQNTEGLYGGIEWTNPN
                     DQVYEALMSHPRFKPNFGWCPREELAVSTRIITKKFATRIIRAAFEYAKKRGFDKITL
                     CEKPNVIRETSGMMLKIAQEMSKNEYPGIWVEDVNIDAMMMWLTKNPENYHVIVSENM
                     FGDIVSDAFAGLIGGLGFATSAQFNPDSGVAVFEPTHGSAPKYADFETSIVNPIAMVL
                     SSVLMLEHLNENEIANRIRKATEEVVSEGKVRAYDMLKMKGTQDVVNQGAASTKQMAD
                     AIIAKLK"
     gene            17401..18228
                     /locus_tag="PKY63_04045"
     CDS             17401..18228
                     /locus_tag="PKY63_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPE99819.1"
                     /translation="MNILVLKYLILILINSAQSVPVKEKMPFKPKMNPPGTVWLRDSL
                     FIDVAPVKNVDYKEFESFVQIAYSKELRDSLKNIPSYGIDMNCFQSYMRLCGPDKQMA
                     AKMKVPVNAPLSWAMNMNEYYNSPHFRHYPVLHISFIQAQAFCEWRTDMVMLLFAVSS
                     KNEKQRTKYYTRVRYRMPTIEEWEYAMEKFKENVYTNMAVYAGERCATIEAVPQKKNL
                     EFVYIPHNIAEMTTTEKTAVGLSWRDADTTDNYSKTVSYAGPRDWLGFRCVCEIVEY"
     gene            18230..19861
                     /locus_tag="PKY63_04050"
     CDS             18230..19861
                     /locus_tag="PKY63_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE99820.1"
                     /translation="MKKLLPFIAVSLLLISCGRKHEVPDNIKLFRYNEAAGIRSLDPA
                     FARDQANIWGVNQLFNGLVQLDDSLHVQPCIAKSWEISDDGLVYTFHLRNDVFFHPSE
                     AFNNQKRKVIPADVEYSLQRLADEKTASPGKWVLGNVACKSDGKLDIIVSNDTTIAIR
                     LNKPFAPFLSLLSMQYCSVVPREAVDFYGKDFGHHPVGTGPFLFKYWKDGLKLVLLKN
                     PDYFETDKSGKRLPCLDGVVVTFIVEKHTAFMEFMQGNYDMISGLDPNYKDVLLDAAG
                     NLKQEHNNKMYMTRIPYLNTEYLGISVHKNGGGTISALQEQKVRMAINYGFDRRKMMR
                     YLRNNIGYPGIYGMIPPGLAGYAEDSTFCFEYNPAKSKQLLAEAGFADGKGLPEILLQ
                     TTATYQDLCEYIQQQLAEINIKVKLDVVPPATLREMVSQSKSGFFRASWIADYPDAEN
                     YMSLFYSKNEVPNGPNYTHFANTEFDRLYEQSVSEPDAKIRADLYAKMNRILMEQSPV
                     VVLYYDMAVRFVSNRIKGLEPNAMNLLNLKYVVKE"
     gene            complement(20007..20080)
                     /locus_tag="PKY63_04055"
     tRNA            complement(20007..20080)
                     /locus_tag="PKY63_04055"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20044..20046),aa:Ile,seq:cat)
     gene            complement(20191..21009)
                     /locus_tag="PKY63_04060"
     CDS             complement(20191..21009)
                     /locus_tag="PKY63_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPE99821.1"
                     /translation="MEPKNDLKLAVIIDADNVPFANVKGLMEEIAKYGVPTFKRIYGD
                     WTRPNLAGWKSVLLESAITPIQQYGYTSGKNATDSAMIIDAMDILYTGRVDGFCIVSS
                     DSDFTRLAIRLRESGMKVFGFGEKKTPDPFIVACDKFIYLEILKTAPLPESAQTQPLA
                     KNKMLVPDGIQKVDHETVSLIRSSVDDIADEDEWAFLGDVGSLLIKKKPDFDPRNYGF
                     DKLTPLIKSLKDYFEIDERVTDKSFIKLVYVKSKKNEARKQKNNNPKKPMDKIF"
     gene            21313..22407
                     /locus_tag="PKY63_04065"
     CDS             21313..22407
                     /locus_tag="PKY63_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPE99822.1"
                     /translation="MSVRRLFVGLIIFCISATALAQTRSLSAAFGYGFTSPHHPDMWN
                     YITGHAHQCHIQYLTPRDTSNHRGGHTLKVIDLCFINPGNPDTMGYSIGLSPQFHFPL
                     NKKRRSFFVLGCGLEYNTKTYTEENCSFNAIGSHLNALIVLGYKHVFELTPSWKLKTE
                     FLFTHFSNGATKAPNLGLNVPTLNLGFDYSWNMSDEKKSKKKLQPDIQSGRWQFVGTG
                     SWKQVKHEGKISPAFCISGEKFFPQTKKSSFGFGSDLILDYSMNNRLTEFSDTIVPFS
                     NNIKIALKAMWTVPIGRLQIHLQAGSYLKNSNFKKEFAFERLALRYFITDHFGLNVAL
                     RAHFAKADAIEFGAVYRIPSKPVIFHPSIF"
     gene            complement(22500..22835)
                     /locus_tag="PKY63_04070"
     CDS             complement(22500..22835)
                     /locus_tag="PKY63_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPE99823.1"
                     /translation="MENAIQTWLEDILKSIDEIYEFLPEKRNYHEFRKDLKTRKAIER
                     NIEIVGEAVNRILIVNPVIDITNARKIVDTRNRIIHGYDSVSEDIIWAIVSNDLMKLR
                     AEIVQLMNT"
     gene            complement(22807..23127)
                     /locus_tag="PKY63_04075"
     CDS             complement(22807..23127)
                     /locus_tag="PKY63_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE99824.1"
                     /translation="MNIISQHIDQINLLCELNSVRSLYSFGSVNTERFDAQSDIDFIV
                     DIDDNDPISYSEKYFNLKFNLEDLLKRQIDLLESKAIRNRFLKSEIDRTKVFIYGKRD
                     SDVA"
     gene            23218..24582
                     /locus_tag="PKY63_04080"
     CDS             23218..24582
                     /locus_tag="PKY63_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPE99825.1"
                     /translation="MKYAVCDIETTGGNNRAGKITEIAIFLLEDGEIIDEFSSLINPE
                     RPIPPFITQLTGITDAMVEDAPKFYEVAARIVEITKDAVFVAHNVSFDYNFIVTEFAR
                     LGYAFQRDKFCTLANSRKLIPGHASYSLGNLCADLNIQLIDRHRATGDARATVDLFRM
                     LDEIGIPDSPKVLQTKLKLHPALMLTRFREIPETTGVYYFFNDKDDLIYIGKSTNLHR
                     RVLDHFYNKKAVRDLRMQELTARVEYEETGSELIALLKESHEIKLNKPLFNRAGRRSL
                     SRFGLYDCEDNNGYLNFYIDATQHQKKAFPLRAFSDKDSALSYLDRIIKRYHLCQKLC
                     GIYDSEGPCFHYQIKKCRGACVGKESPEEYNERAYQALDDIQMKNETFLISEGHAVMD
                     KKPFVLVENGTYKGYGYLPENESLSSVEECYMHIQKPAIIDKDAHSIIQSFMRLKKFK
                     MVRF"
     gene            complement(24643..25569)
                     /locus_tag="PKY63_04085"
     CDS             complement(24643..25569)
                     /locus_tag="PKY63_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPE99826.1"
                     /translation="MNQDRKTNLTRIGVFYDGNYFLHVSNYYNYVHNRQSRISISGLH
                     TFIRQYVGQEEGIDPQYCQIVDAHYFRSRPGAKEASMKGNQLYFDRLFDDILMSEGIT
                     THYFPVRTVQGVKMEKGIDVWLALDAYDLNLAHRFDVMVLIASDGDYVPLVRKVNALG
                     TKVMLVSWDFEFVDDFGQQKMTRTSQDLLREVTYPVAMHDLIEDPDFISNPTINGLFV
                     PRSEKKDFDLFESEDFIELQGDGIERTSSILSLKNGYGFIKYPPNNLFFHFHTLVETD
                     MTELKPGDEVMFEIAKNEKGEDVAVKVKLLKD"
     gene            25694..26200
                     /locus_tag="PKY63_04090"
     CDS             25694..26200
                     /locus_tag="PKY63_04090"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPE99827.1"
                     /translation="MIEIVKGDITKLGAECIVNAANTSLLGGGGVDGAIHRAAGPQLH
                     EFCKKLKGAQTGEAKITPGFRLPAKYVIHTPGPIWRGGTHNEPELLRLCYFNSMQLAL
                     KNKIATLAFPSISTGVYGFPFDLAARIAISTIHDFISKNPAAPDVFLVAFSESDFDTL
                     KKIYSEML"
     gene            26319..28550
                     /locus_tag="PKY63_04095"
     CDS             26319..28550
                     /locus_tag="PKY63_04095"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HPE99828.1"
                     /translation="MKKSRKIFLIALAVIVLGIGGTAAYFYFKTSPMRAIWNYVPQDA
                     VYIIETDNLTKGWNTLAESNMWQHLISDPLFEDISQSAFTLDSLIRGDETMDMLFADR
                     PLLVTCHLLPNQDFDFLFLIDLKQAGKFAFIKDYVGGIVGTFGYEMTREKFEDTDILI
                     ISDKTSDDIFYVSVIDNVFMASYNKSLVQNSITSAKTPDQWKNNTAFQSVVSSTSGNN
                     LFRFYVNYHFMAPYIGYYLSDEQELLDELKRIFNYSAFNINLESERLSFSGSTILKDS
                     AESYIHMLSGIERGPLQAYRIIPSDYALYLSVSFDDYMDLFNNLKSEFTFKDSAGAES
                     YEKTLKKMEKLFGINLEEDFFSWIGTEIAMLKLPPTPNARENDMVAIIHTKDIELAMN
                     GMEKIVKKVKNRTPIKIDDSEYKNFKIHYFGMNGFFKMFFGKMFAKLEKPYYVFMDDF
                     VVFSNSPSNLMDMIDAYEKGRLLANDEEFMNFFGQFDDEANVSVFLQGPKIYSHLYYY
                     AKKDQKANIRQSREILASFHNIGFQLSSKKKGFFSNTLIVEHSADALFAAELEEIENS
                     AEELYASEFDSLLAIELPDEFDNGKTCRIYYEDDSTQIKAEGRIVDGSREGMWRYYYK
                     SGEIWGSLMFEEDEPIGKGILYYDEEDGGTKAQTEFEDGKIEGTYLEYYSNGESKTSI
                     EYSEGKPDGEAKFYYDSGSIKIEGEYKVGLKKGKWRHYTETGDLMDKEKWKKDQKKIK
                     NAK"
     gene            28563..30029
                     /locus_tag="PKY63_04100"
     CDS             28563..30029
                     /locus_tag="PKY63_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPE99829.1"
                     /translation="MLTFLISVVVLIAGYLVYGLLMEKVFGVDKNRSTPAFTKQDGVD
                     YVPLPWYKIYLIQFLNIAGLGPIFGAIAGAMWGPSAFLWIVFGSIFGGAVHDYMSGMM
                     SLRLDGLSLPEIIGKYLGKPMMQFMRAFTVMLMILVGAVFIMGPAEILAGMTAESFSI
                     GNLDISLNLTFWAIVVFVYYFLATMLPIDKLIGRIYPLFGFALIFMALAIIGAILFGD
                     YHIPEITGNLYNQHAKGDAMPIFPMMFISIACGAISGFHATQSPLMARCIKNEKYGRR
                     IFYGAMITEGILALIWAAAAMAFFGGVEQLNNTMLAQKSNAAWVVNEISNTLLGRFGA
                     VLAILGVVAAPITSGDTAFRSARLIVADFLHLSQKKIFHRLFIAAPLFLGGFVLTQID
                     FGVIWRYMAWSNQTLATITLWAITVYLVLDKKPWIVSFIPAAFMTMVTSSYIFFAPEG
                     LQLSYHLSASIGAGITLIVLMLFFIWLNHARKKSAPKL"
     gene            30166..30531
                     /locus_tag="PKY63_04105"
     CDS             30166..30531
                     /locus_tag="PKY63_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99830.1"
                     /translation="MKKFIFLLLLLPVVFLTSCDKNSQILVFKKDITYKVKGTATEIL
                     VTYTNDKGETTLTGLSTGTIPWSYEFSAKPDTYVYLQAKNATDTGTVEVQILQRDAVL
                     ASDENDLPFGAATCSSFVK"
     gene            complement(30533..33286)
                     /locus_tag="PKY63_04110"
     CDS             complement(30533..33286)
                     /locus_tag="PKY63_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE99831.1"
                     /translation="MNSSVEDIYKFLEDAGDADVSAKKIIEHLDAVQLRQFGKKHHDR
                     YVELLEGFSTFKDIQSLSDFLTAFLYSVIGQEHAVTVISLCNNENTEFHSVSMDGSSD
                     LLDKVRKMAESGQLTFDMKNSLDDISPMFEDRLQQIESLYHFVHKSIPEEACPAVEEF
                     INLHYFYGYPISDGNKIIATISMALCDCLPEVKFIEIDRMIRLARQFYILLFNFQRCD
                     QLNRHMTSVFDATDLAFGLFSSDGHLIKANNSFNALFKLYKSPHLFDPEFINVLGIKD
                     IDQLKQGSEVLMDVSQNVFRNLPALAEYSSGRIIPVKATDTTITSFIFILHSRKAELQ
                     ILETVKKNEQKYQRIFNYIQNVYFEIKLDGTILEISPSLYHYTNINPATLIGTNILTL
                     YQNPLQRDAYVKEILSTGRVDNFDIDIRLPDGKVFNTIVTATIVDKGNPEERITGSMV
                     DVTELKNKARAVVENEIKFKSLFDNAPIGFMICDVEGEILEMNPSFLRIFGLSYIDKQ
                     QETNLLKNNMANILGIADAVKQVMQTREPLFTEYQYAFPDGVLHSFKVKISIIPDESG
                     KSKYVLIIAEDVTEIKVKEQELEITRERFLDIYNNTSDLIYTMDFQGNFTSVNPVAEK
                     WLGYRFQDLKDKNMSDFISHDSYKRAEEQIKLKLSHATEHSTYEVTAFTRTKEKMILE
                     INSFLRYKDGNPIEVFGIARDITERKKHEEFITMALRERERLIMEVHHRIKNNLQLVL
                     SMLKMYAGTYDNEKILQTFRDIIQKIMAIATAHEDFYFSTDFKDIDFRNYLKTIVINS
                     IEQFDTLSRVKYTIDSDELNATIDEVVPLGLIVSELLSNSIRYGIGSDGNVQLSIVFK
                     NQGSKHELVISDQGPGIPAALLQNKEKSLGLSMVAMLAENQLGGRYQIDSNEKGTTIK
                     VEF"
     gene            33362..34642
                     /locus_tag="PKY63_04115"
     CDS             33362..34642
                     /locus_tag="PKY63_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE99832.1"
                     /translation="MSIILKNANYYDFRQDKILFTNILVESESKKKLTISSDVNDLLD
                     NQTDVRIIDCFGKLVMHSFVNAHHHSYVSLTRTHKLTNTDDFEFRLKTGAWKIDQFLD
                     ADVLEACALYSAIESLKNGVTCVIEHHSSQNYIKGSLETIEKAFKKVGLQFLPCFEIS
                     DRKSYSASDEALDYTDSWLSGHAGLVGLHASFTVGSTTLKNAVDMANKHKTGIHIHVA
                     ESTIDQEQSLKEYGVTVVERLYDAGVLENTKTVLAHAIHLSNEEAELINKSGVFVVEN
                     MESNLRSNVGFFNSEGIGDNIMLGTDGLHGNMLRSAKMAFLAGQGFDHIDPSIAIQRL
                     RAGNDYFLKNKICGDADNNLLVFDYPSPEEVTPSNMAMHMIFGMESKHISHVISNGNL
                     VVENGRLTMVNEDDIIPFIRQMTRKLIKQLEKSK"
     gene            34642..36726
                     /locus_tag="PKY63_04120"
     CDS             34642..36726
                     /locus_tag="PKY63_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPE99833.1"
                     /translation="MMFKSMRFVTGLVLFLALCLFSGNLVNAQIVSPEEANTVAANFA
                     TQRLGNNFAPYFSEFYTAEYNGQTTYYVFNLFPEGFVIVSANKNVVPILAFSPENNCP
                     KNHSIPALSALLGNYSKQISETSELKNYRNPENQQLWSDYLQGVIKSPKGQVEPLLTT
                     TWDQPTYYNEFCPADPDGPDDKCVTGCVATALGQLVNYFRWPYSGTGSYTSEDTVYGS
                     LTVNYAAAHYDFNEMAIDLTRSNPETAELIYNIGVGVDMHYGPDGSGMNNHKAAHVLK
                     TFFKYADSTQYIFRDSVSLNWDSVIISHLERGIPMYYAGWGDTIYMSGHAFVVDGYQD
                     TCYFHINWGWGGSSDAYFYTSDLSPGYNFNLMQELVINMYPDGPYPYYCNGTDTVSSR
                     DGTIDDGSGPLFGYRNNTDCSWLIAPDDSISSIELSFLRFDTETGNDILTIYDGENTS
                     APVLGSYSGSSIPATIESSGSRVFMTFTSNGSDSAGGFLVSYKINTPYTFCDALTIIT
                     AESDSIEDGSGQYLYHGNNFCRWKIQPTSTEPVLLTFSEFDLDSTDYVKVTDHTSGAV
                     LGEFKGNQLPPVLYSGNGTMTITLKTTATAHAQGFKCNYRTSPVSVADISKPDIMLFP
                     NPAKDFITIAGAEYDCEVIVFDLHGRRVYSSIQQAQGGYIVVPVHQLSNGMYLLSISN
                     KTGIHSLKFFRE"
     gene            36730..37074
                     /locus_tag="PKY63_04125"
     CDS             36730..37074
                     /locus_tag="PKY63_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPE99834.1"
                     /translation="MKNIIVVGDRVLIKPKKESEQTRSGLYLPPGYSQKELLQEGYVI
                     QVGPGYPIPSEEIDEPWKSGTGENIRYIPLQARQGDLAIFLQKNAVEVMIEDEKYFIV
                     PQNAILLLQRED"
     gene            37159..>37728
                     /locus_tag="PKY63_04130"
     CDS             37159..>37728
                     /locus_tag="PKY63_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99835.1"
                     /translation="MKKNIFLILILLYTSATAQHMRIPDGTWVSDTVCKTYLNDTLLC
                     DDCCETYINPFTFTFSNGRLYCDRETGNSNSGYLISEYKMSRNTLRLKKTMMVDSSLQ
                     IINPRNYYLLYPKKYLYECDNDSVFTLINKWDNCKSIINAHKAPTQTFLTLVEIKNIN
                     QQRLDSATTKKIVLIKNDSTLKKVILKTNG"
BASE COUNT        11153 a         8467 c         7251 g        10857 t
ORIGIN      
        1 accttaacct ggctgtttcc ctgccgaagg cagctcaacc ttaacctcaa ccttagcctg
       61 gctgttcttc ctgcctcagg cagctcaacc tcaacctcaa ccttaacctg gctgtttccc
      121 tgccgaaggc agctcaacct taacctcaac cttatcctgg ctgtttcccc tgccacaggc
      181 agctcaacct atgccttaac cttagcctta ttcttagctc ttcatcgttt taataatttt
      241 atttaagctg ttaattagat cctgatactg ctttatcaat ttttcaacaa cttctttgtc
      301 aaaataaccc actgcacagc cataccgcaa atgatgcttc gtttcataag ctgatccgcg
      361 cgaaactaca taaaaatatg ctttatcatt accggtaaca cggccaaacc cttcagaaat
      421 atttgcccca acactattcg ctgaacggcg tatctgagaa gtaagtccat aatcttcaga
      481 ttttgggaga ttggaagtca gctcaaatat ttctgtggat aaatcaaagg ccgttttcca
      541 gacaggcatt tgagtaaatg aagtgtacat tgctattttg gttttctatg tctattctct
      601 caggttaagg ttaagtggct tagtctcaga tgaaagcttc tcggcttttg cctcaacctt
      661 tgccttaacc ttcgtattcc tgcctcaggc agctcaacct caacctcaac cttaacctgg
      721 ctgtttccct gccgcaggca gcttaacctc aaccttaacc tttgtcttat cctggctgtt
      781 cttcctgcct caggcagctc aacctcaacc tcaaccttat cctggctgtt tccactgcct
      841 caggcagctc aacctcaacc tcaaccttat cctggctgtt tccctgccga aggcagctca
      901 accttaacct caaccttatc ctggctgttt cccctgccgc aggcagctca acctcaacct
      961 caaccttagc ctggctgttc ttcctgccgc aggcagctca acctcaacct caacctttgt
     1021 cttatcctgg ctgttcttcc tgccgcaggc agctcaacct taatctcaac cttaacctta
     1081 ttcacagctt cgccgcgtca gccccatcgg acaaacaacg gaaaatattt tatatcagca
     1141 cattacgacg ccgaaaacgg cagaaccaga aattttccca gctcttccac ttctagcaca
     1201 aaatgtgttt tccctttcaa acgcactacc ctacccgaaa tacctttcaa tggtcctgca
     1261 ttgattgaat atcttgtccc cggcctcggc atttcatcta ccatttcggc catcaatccg
     1321 gcctcggcaa tccggcggat gtcagacatc gtctgctccc gcaccatggc ggcctttcct
     1381 tcaaagcgga tataacaaac cattccgtct gtattgatgg catcgaaata ttcttttcga
     1441 tcgacctgta caaaaatata agatggaaac aatggctcct caacccattt gcgacgatcg
     1501 ctccattgct tcttggtttt ttttaacggt aaataatgat tgatgccttg ctcagataaa
     1561 cggtcagaca ctttcttttc cgcacgggga gcggtgtacg caacatgcca ttgccgtaaa
     1621 ttgtctttat catttatatt catacactta accagatatg tagccttttc ctatacatag
     1681 atttaaacat gcaaatgtac atttttcttt tgattcaaaa tgggtttgag tatataagaa
     1741 ttataaaatt accgctttat ttgcaaatca tgaatctgcc caattcatga ttctctttaa
     1801 atatgcacat ttaatcaata atatttatca ttttgtatta gttaatgata gttctatata
     1861 aacacaaaaa gtgaaacatt aaaccatcca taagccctct tttccacact tataatgtca
     1921 taactccccg acaaactcgc tgtaacagga atcggagcga cttcaggcag tgtgccatat
     1981 gcctactttg tcaactgttg aaacaattct tcaattgaat gtttttcctt cgttaaagaa
     2041 aggactttgc attgattgcg tacagcaaag tcaaaaattt ccttgcgaac atcaatactc
     2101 tgatcagctt ctactgccca catgttatcg cccttagtgt ttgcattttt cacacccttc
     2161 acccccaata aagcttcacg agttactttc cggtcgaatt cgaccagaaa caattcagca
     2221 gcagcaaaca tcgacgataa atcaccagtc tttccatcgg caacaatttt tcctttgtca
     2281 atgattacaa cacggttgca gatagcttca acttcctgca tgatatgcgt tgaaagaaga
     2341 atggtctttt cagaccccag tttcctgatc agatcacgta tttcaacaat ctgattcgga
     2401 tcaagaccac tggtgggctc atccataatc aacacttcag gattgtgaat aagtgcctgt
     2461 gccaatccca ctctctgttt ataacccttt gaaagggctc cgatccgctt gtgcatctcc
     2521 ggcttcaatc cagtgatttc aatcatttca tccacccgct gtttcttatc tttcagattg
     2581 tgaatccctg caatgaaggt aagaaactcg cggacataca tgtcgtaata caatggattg
     2641 ttttcaggta aatagccaat catgcggcga acattcaaac tttcatcggc acaattcatc
     2701 tcattgacaa aggcctgccc acttgtggga ggaatgtaac aggtaaggat tttcatcaga
     2761 gtggacttcc ctgccccgtt tgggcccagt aatccaacga tttcaccttt gcctatttca
     2821 agcgaaacat tattcagtgc tttctgctcg ccatatactt ttgttacctg atcgaccctg
     2881 attgacatgg atttgaaatt tgatacaaaa gtaattgatt cttgcaaatc ataagctgaa
     2941 agttttgcat cattcttaac cttggttttt ctttctgcaa gcagtttaac ctcaacctta
     3001 actttgtact atttcttctc aaacacatat ttcagaaact tcggcttgaa ttccagcatc
     3061 acataggccc aatgcgccaa tttagtgtgg tctccacctc tcaacagctc aagtgtcgtg
     3121 ctaccgctga aaactgaata ccccaccgac aataccatgt cttttgccaa tgcggccttc
     3181 catgtcaaat ctatttcgtg cgccagaaac tgatcgatgt ttgccccacc tgtgatggtg
     3241 ctctgctgag ttgcaaaata atgataatca agcgccagcg aatgtatttt tgaaaatttg
     3301 aaatgtccct tgagataagt atcgataaaa cctgctccgc ctgtgctggt cgcaaaattg
     3361 gtgaaataat ccatatgccc tgcaaaggca tgattcgacg catattgcgg aatgaattgt
     3421 tcatatttgt ctgtggttac atcattgccg ctcatcattt caccacccag tttcaccgac
     3481 catttgtcag ttttgtattc ggcttcggat gcggcaaacc atgctgcaag ctcagttccg
     3541 gaaatattgt gaccggtctg ataatatccc ataccactga aaatgaattt atttaatgta
     3601 tatttcaatt caacaccttg cgtactcctt acatagagcg ctttcccgtt caaatcctga
     3661 aaaccgtcag caaatcccat cactgaatat tcaaagtttt ttatcttctg ctcataacgc
     3721 aaaactgtca atgtcttata attgtccttg atttcagaaa cattgttcaa ataatccgtt
     3781 ccaaaaatat tctcggcccg ctgattgaat gcctgaacaa aatacacctg cttagtccct
     3841 gatttccatt caagcataac tgcatcatgt tcctgcgatg tctgcgacca gttgttgcca
     3901 ctaaacaatc gttgattgcc aaacaccaat tgatgtcgtc ccatttttat ttttaatgaa
     3961 tctgcgattt tcatacgcac ccaggcctca agcataccta cagtggcctt gttgctttta
     4021 aacaattcat cgccccatac acgcgattcc tgcaatatca gcttcgtttc aaacttctcg
     4081 cttgtataat tcaatccaat acgattgcgt tgtgcaacaa aacctgcagc ttccgtattg
     4141 ctgtctggta gctgtttgta tccatttctg tattcaggtc tgaaacggaa gtcaccactt
     4201 aattcaaatt cttgcgccat tgagaaaaaa ctcatgccgg caaaaaaaat aaagaaaata
     4261 actctgttca tatacattgg tttttgtttt attttgacat tttgaaatac tttatctttc
     4321 gcaattcaag gtgacaaata tcattcaatc aggacttttt caaccatatt tttacgtata
     4381 aacttcaact acgaacaata ttgagacatg accataagct ctgttctcct ttgatgcaac
     4441 tccaatcgca atcggaatgc tatgcgtaac atgttccttc tcaacctaat cctgaacctc
     4501 aacctaaatc ttaacctcaa tcttaacctc aaccttaacc tcaaccttaa ccttaacctt
     4561 aacctcaacc ttaacctcaa cctcaacctc actgaccact aataaaaaac tccctccagg
     4621 tatttcttcg tcatttcatt ttccggattt gaaaacaaag tttgtgaatc gccactttca
     4681 atcatttcac ccatatacat aaatatcacg tgatcagcta ttcgcctggc ctgacgaaga
     4741 atatgactga ccagaataat cgtgtaatca cttttcaatt gcataaatga ttgttcaatg
     4801 gcctgcgtgg aaatgggatc cagtgccgaa gtcggctcat cagccaaaat aacttccgga
     4861 tcgactgcaa gcccccgagc caggcaaagc cgctgctgct gcccgatact gagtgttgaa
     4921 ggagattgat tcaatcggtc tttaacttca tcccacaaac aagccagttt cagataatgc
     4981 tcaacctggc tcctaacctc cgttttacgc gaaattcctt tcaagcgtac agcatatgca
     5041 atgttctcaa aaatactaag aggcaatgga tacggtcgct gcgaaagcag tcccatacgt
     5101 tgacgcatag cagacacgtg ataaccacgc agcgaaagta tttcctgtcc accgaaatga
     5161 atctgcccct taaccttgag cccattcaga tcagtcaatc gattcatcga ccgcagcaaa
     5221 gttgtttttc cgcaaccact cggccccata ataacagtga tggtttcacg cggaattttc
     5281 accgatatgt tattgagcaa ctttgtctca ccggcatgta cgctcagatt tcgtatatca
     5341 atgatgggtt gattatttgt catgtcttaa atggtttttt tgtaaacacg tccgataata
     5401 cgggcagaaa tactgatgaa aagaatgata atagttagaa tcaatgccga agcataagca
     5461 cgcccctgta cttcttcgac cggggaactc aattgaaaaa aaatggccag cggcaaggta
     5521 gctacctgct gatcgagact ggtaggaata taatcggtat aaccggtagt aagcagcacc
     5581 accgcagcat cgccgatagc ccttccaaaa gataacaaaa tagctgtaac tattcccggt
     5641 gctgctttac gaagaaaaaa gcgaaacgcc gtttctgttt tcgtgcatcc catgctgtat
     5701 gcagcttcgc gaattccatt cggcacccgc tgaagcactt catccatcgc tctgatcata
     5761 ataggtgcaa caaaaattcc aacgaccact attcccccaa gcaacgacgt tcgaattccc
     5821 aatgcaacca ttattgtaaa tccaaatgca ccataaacaa tggatggaat tccccacaaa
     5881 acatcgagaa aaaaacggaa aaaagatacc gctttgctgt tgcgctgaag aaaaacattc
     5941 atatacaatg ccaccggaac gctcagaatt aatgccaaaa aagtcgcacc cagagccaaa
     6001 tacaacgatc ctataatggc attcaaaata ccaccttctt ttccgaaata ataaccaccc
     6061 ttgggcaacg aagaaatcat tgaccagttc agatatggaa ttccacggac aacaatgccg
     6121 tataaaatcg atccaagagc aattgcaatg ataagggttg cacttatcat caaggttttc
     6181 attacaaatt cttcgaaata tttatgcttc attatctgca gtttttgttg taaaacgacg
     6241 gatgatcaaa cgcatcagaa tattgaatac aacagtgatt accagcaata ttaatgcggc
     6301 aaacatcaat gcactatcgt atttaggaat actcatcatt tcgccgtaat tgtttgcaat
     6361 cagcgccgga agcggataac cagggcttac aatatttttc ggaatagcaa tggtattgcc
     6421 tacaaccatc attacggcaa tggtttcgcc aatggctcgt gcccaaccca aaccaatggc
     6481 agaaataata cctgcgccag tttttttcaa tacggttact ttaaccattt cccattttgt
     6541 tgtacccatt gaataggccg actcacgcaa ttcaagcggc actgtggcaa aaagctccag
     6601 taagatattc aatacaaacg ggattatcat cactgccaat accagactac cagccaaaac
     6661 agaataacca gtactttcaa tacctgcaac gggtgctatc acatttctta caaatggaac
     6721 tataaaaaga atgccccaga ctccataaat caccgatggg attccagcaa gaatatcaat
     6781 caccggctgc ataaatccaa gaatgcgtcg atgcgcatat tccgaaagat agatggcagc
     6841 aagaatgcaa accggagcag aaatcaatat ggcaagaact gaaacatata aagaaccaat
     6901 gataaaaggt ctgaaaccaa aactaccggc agccggcttc cagtcatcgc ccgacaaaat
     6961 atttacaaat ccgttttcct gcaacaacgg cagagattta tcaataataa catacaacat
     7021 aatcaaagga acaatgatga taatgatttt cgaaatcacc atccatgcaa agcccgcgta
     7081 atcacgcata cgacgtgttg caatcatcag tctaattttt tgagttcggc ttctatcatt
     7141 gcatcaggca actgtacata gccgctttcc tcgacccatt gctgcccttt tgtaagaata
     7201 aatcttaaaa atgcaactac aagctgatta tcgggtttcc ctttacaaat aaaatacaag
     7261 gggcgggctg gcggctccgg gaattttcct tctcgtatgg cgcctgtcag caatttcatg
     7321 tcgctgtaga ggttttcttc cttgtcaatt tttccgtttc cattcatgtc aatcggaatg
     7381 atgccaatgc catcctgcac tttgccggtt ttaaaatcat aggcataaat aatattattg
     7441 aagccaacgc ctttaatgtc attttttaca gcatcggcaa tgccgggatc accaaaaacg
     7501 ccaacccctt tcaaatcggc ttgctctcct cccataaact tggcccacat ttcggctgca
     7561 ccacatgcat cgctgcgggt aaacaaattg attgctccgc tgcctttcaa ctttaactgg
     7621 ttccaattat taatttcctg agtcatgtag attttttcaa actcctcttt tgtaagcccg
     7681 cgtgtcacaa gctctgtata taccggattt ttcgcattaa tcgtcggcaa caccacgtcc
     7741 tttgccaccg ctattttcca cgctccattg ctttcttctt ctggcgaaac actacgcgaa
     7801 aacatggcca tgtcaaccat tccattcaat acatcggtca tgcccttgcc cgctcctcct
     7861 gccgaaatat caatccttac atcagggttt tctttcataa actcctcagc ccatttctga
     7921 gccatcggat acaaagcaaa tgcacccgaa atgctaatgt ttcctctgag ttgatcagat
     7981 ttctcctcct ttttcttgtt cccgcaaccg gttaccacta tggcaaatac caaaaaaatg
     8041 actgaatatg ttttcattgc ttttaaattt ccggcaaata taatacattt cctatcgata
     8101 tagtatagtt acaaacattt cttatatatt aactcttatt taactctctg cttttgtaat
     8161 agaattcatg gcaaaagtga tttgaacacc aaataagcaa cttttcaggt tgttttcaga
     8221 tattttaatg catggactgt gatgaattca caatgaaaga tatatgatgc cccatgcttt
     8281 tgttatcttt gcaaaaaaaa atggaatttt cctggtacga atttgtcgtt ttacctcttt
     8341 taatattttt ggctcgtata gccgatcaga gtgtaggcac actgcgtctg atttttttag
     8401 caaggggtat taaattctgg gttcctattc ttgggttctg cgaagtcatt atctggttgc
     8461 tggcggttgg acaaattctc aacgatctga caaactgggt tgccatcatt gcctacggag
     8521 ccggatttgc agcaggaaac ctgataggga tgtggattga tgaaaaactt tccatcggaa
     8581 cattgattgt tcgcgtcatt ccgaaattcg atacaacggg acttattgcc gatctttcat
     8641 cgcgcgattt cggtgttacg gtttccgatg cttctggcgc tcgcggaccc gtgaaaattg
     8701 tgatgtccat catcaaaaga aaagatctta caacttttgt cgaaatcgtt cagaaacaca
     8761 atccaaatgc attctatacc gtcgaagacg tgaaagccgt ttcagaaggc gttttccgcg
     8821 catcgaaacg cagatccatt ttctctttca acagtcgcat cgggaaataa aatccttccc
     8881 gacattctgg cgtcagtttg gaaaggtcaa caataatgca ttactgacct gaaattccga
     8941 cagtgggcgg acaagctatt aaaaaaaagc atcaattgca accaacaatt tctttctcaa
     9001 aatcaatctc gggcgaatgc caaaatgttg atttattact tgaacttagg atatgaaata
     9061 gtcaattgta gcaatttcat tgaacgtttt gttggctgat gcctgacaaa acaggcaggc
     9121 atcgcatatc tgtagaaaaa ccaataccga gcaaacgatc cgccggctgg cggattgcat
     9181 ttaaacaagc attgtcagaa gacgaagctc cttatatgcg actcctccgg agtcgagatt
     9241 ttgttttata tgggctacag atatatgact cctccggagt ctcccaaaaa taattgaacc
     9301 gatttataaa tcactttgat ataatttctc agactatttc gctgcaattt ttttataaaa
     9361 cgcgagcatc ttctcaatcg tgctgtcaat atgatactcg ctctgcatgc gctgtctggc
     9421 agcttcggcc atttttgttc tttcctccgg atattcatac atatactcta tagcccttgc
     9481 aatagccgac ggatttttac gcggaataac caatccgctt tcaccatcaa tcaccattcc
     9541 gctgttgccg ggaatatcag taataacagg agccacaccc agactcatgg cttcaatcac
     9601 ggatttgcag atagcttcgc cataaatgga actcaaaaca aacacatctg aatttttaac
     9661 cagccccatc gaatcactgc gatagccagt gaaaattatt cgctccttca taggactggc
     9721 ttcaaccaat tttctgattt catcagaata aagtccatta ccaatcaata aaatatacaa
     9781 tgacaaatcc tgttttattt cgtatgtcgc tttaataaaa tatttcaaac ccttcattgg
     9841 acgcgcgttt gcaaccatgg tcaccacaaa tgaaccagaa ggaattttga attcatcata
     9901 cgaaacagcg tccacatttt tataccaatc cggatgatga cctttggtaa tgcagaatgc
     9961 attttttttt ccgaagattt tatttcggtt aatcagatcg gctacagcag gtgcaataca
    10021 ccagacagca tcggcacggg gatgcagaaa tttaaaatat gccaacggat cccaccaatg
    10081 aatatttccg cagtaaccac gatagagaac cacttttaca ttgagtcccc gtgctgcttt
    10141 cagaccattg atgatggctt tgccattaaa aagatgcact acatggtacg caccgacaat
    10201 caattctttg cggataaaat caatttcgct gcgatcaaat tttttgtgtg gttgaaaatc
    10261 aatcacgcga atgccggcag cttcgaaaac gggcgtatat tcagcagttc cgggtgtcat
    10321 aatggtaata ttcactccgg cttttttcag tccaagcatc aattctgctt cgggtctcga
    10381 agagttgagc ccgctgtaat tgctgatgac gaggagtttc agattcgaca ttgatacaaa
    10441 ggtaattatt ttgccctaaa ccatcgaagc cataaactct cgaaaggttt tgaacccttc
    10501 gagagttggg gctgcagcaa aaaaaaccgg cctgcttgcg gcaggccgga cccaattaaa
    10561 aacaaagcat taaagcttta ttttcttctc gatctcttcg acatacaccc gaaaatgttt
    10621 gtcggtatcg ataagatttt tcactgtgcg tacagcatgc aagacggtcg cgtggtcttt
    10681 tccacctata tcaacgccaa tgctggcgag agaactcttg gtcatcgttt ttgcaaaata
    10741 catggccaat tggcgtgcct gtacaatttc gcgcttacga gaactgctgt gcatttgttc
    10801 aacggaaata ttgaaataat cacaaaccat tttctgaata tagtcaatcg aaatctcacg
    10861 tttcgagttt cggacatact gatcaatcat ggttttagcc aggtcaatcg taatgggttt
    10921 gcgattgaaa gaagaatgag ctaccaaaga aatcatggct ccttcaagct cacggatatt
    10981 ggttgaaatg ctgtaggcaa gatattccac tacatcgtca ggaatagtaa taccgtcttt
    11041 gtgcagtttt ttgcggataa tggcaatgcg ggtttcaatg tcaggcacct gtaaatcggc
    11101 tgccagtccc catttgaaac gagaaagcaa tctgggttca atatcttcaa gttcaaccgg
    11161 aggtttatcg gaagtgagaa taatctgatt gcctttttga tgaagggtgt tgaacaattg
    11221 aaagaaaacg tcctgcgttt tgagtttatt ggccaggtta tgaatatcgt ccatgataag
    11281 tacatcaatc atctgataga aatgtacaaa atcattggtg gtgtcatctt tcacagagtc
    11341 gataaactga tttaaaaatt cagaagtctg aacatagaga accgttttat cgggaaaatt
    11401 tttcttgact tcaataccga tggcatgtgc aagatgcgtt ttgcccaaac cggtatcact
    11461 gaaaataaag agcggattga atgaagtttt accaggattt ttggcaatgg ccaaaccggc
    11521 agaacgagca agacgattgc aatcgccctc gacaaaatta tcaaaattaa gactttcgac
    11581 gagctgcgaa ttgactttga ttttacgcag accagggata ataaaagggt ttggaatttc
    11641 tttggaagtg ccgcggttaa tatcgatggg aatatccaca gaaggatttt tcaaggcgtt
    11701 tcggtcagat gatggataat taacagagat tccattttgt gtagcatcta ccagaatatt
    11761 gtactcaagt tttccatcct gaccaagttc tttgcgaatg gtttttttca gaagatcaat
    11821 ataatgttct tctatccatt catagaaaaa gctgcttggt acctgaatag tgagcacatt
    11881 accatcgagt ttcaaaggtt tgatcggagc gaaccatgtt gaaaaactat ggtcgtcgat
    11941 gttatccttg ataactttca gacaattggt ccatatcgtt ttaatatctt ttgccatttt
    12001 caatcaaagg gaattgaata tgttagcgga gtgcattatt tttgaaatca agtaattaac
    12061 aacggtcttg gtcatagtaa gtattttcaa gtattcgtga gcaatttgag agggcaaaga
    12121 tgagtaaaaa aagtttgata aaaaaatttt tttatacttg ctttttaaaa atattatttg
    12181 tataggcatc gacgaaaatt aaatcaccag cagaagaaaa ataacaatca atgcgcgaca
    12241 tacgtacacc agcataagcg ttttgagtaa tcagcacttg tttgttgtcg gaattgaaaa
    12301 ctgaaacagg ttttgataag agcgtatggc tatgcccgcc aagaataaaa tcaatatttt
    12361 tactttgtct ggccagatca taatcggctg tttgtccatc ttcatcggct ttgtatccca
    12421 aatgggacaa acagataata atggagcatt tttcttcctt cttcaggaag taagccattt
    12481 cggcagcagt taccacagga tcgcggtatt cggtatcgct aaaagtaatc ttgcttatca
    12541 gcccataagg ataaacaccg agaccgaaaa caccgatttt tatttttccg aaagtaaaaa
    12601 tcttatatcg tttcaccgat ttgctgagtc gcggatgttt aaagatataa ttggaagaga
    12661 taaaagggaa agtagcatat tccatggcat cggccaggcc atcaagacct ttgtcaaatt
    12721 catgattacc aatggtagca gccgaataat tcatttccga catgagtttc atttcaacct
    12781 ctccaccata catattataa taaggagtcc cttgaaaaac atcgccggaa tcgaaaagca
    12841 gcacaggatg gccttcgcca cgtattttgc taatcagagc ggcacgcgca gccatacctc
    12901 ccattcccgg aaagcggtga tgggtttcgg gcagcggatc aatctgactg tgcatatcat
    12961 tggtctgaag aagaatgatt ttttttgttt tcttttcact cccggcctgc agaacactgg
    13021 gaagagccag cgaggcacct gcggccagca tcgttttcaa aaagtcacgt cgattactca
    13081 tacctgatcc tcccatccgt tttcggatta attgtttttt tctgtgcagt aagatcgcgt
    13141 atgccggaaa taaaaacatc gcgcatcagc aaacctgttt gcacataacg aacattgtcc
    13201 gacaaaaaac tcaaaccatc accaccggtt gcaagataat cggatgtgat gagaatgtaa
    13261 tttttttcag catcaaccac ctgcccttct atttcggcgt ttgtccatga agttgcattc
    13321 agcaccagac gcaaaccacc aacaggcata cctccctttt gtgcaataat gtcaaaaagc
    13381 ttcataagga cacttccctt caattcaata atgacaattt cattatcaaa gggcattaac
    13441 tgaaaaacat tttctatcat aatttcgcct ctgggcaaag atgaacgcaa cccgccatta
    13501 ttgagcaacg acatcacagg caatgaacgc atcgaatcgt gagatgtgct tttcacacgt
    13561 tcaataagca aatcggaaag aaaattacca agtaaacttt caggcatgcc cttttcaagc
    13621 atcgtttcgc tgtacccaat cacttctttc atcgaagaat caagttgctt gcgataagga
    13681 gctatggttt ctacaatgga tgaatcgact cccaatgaat catccacaat caacgaaaca
    13741 gccgtactac tgctcaaaac cgcagttttg cacgatatca gcaccgacga caataataca
    13801 accgaccaca taaaagcggc cagaatcaaa attcttttat ttgttttcat gaagttccaa
    13861 atgtaagatt ttttattttt cacactaaaa aattaaatat tttttgattt ttgaaaattt
    13921 aacacttgaa aacgaaaaat ttaacatctt aagtttttcc tcttttgtct gcaaatttaa
    13981 tcgttttata tctgtaatca taaacaatta ctaaaaaatg cgctaaaaac agacaagatg
    14041 tgcattttac aaacttacat caggattttt tatgcttaat tcccatattc atcatactaa
    14101 taatatttat tatatgcgat atatgtaaat tgttaatata tttactgatt tacttgttgt
    14161 ttttagtgaa tttatggttt atatttgtaa ctccattcag aaaaaacagt acaagtgttc
    14221 atgaaatttt caaacagatg aaactactcc acactctgtc attcgtattt ctcgttttca
    14281 gtctgttgtt tgcaaacaac accggattgc ctgatataga gcgcaaagaa ctgaaagaaa
    14341 ccaatcagga aaatccggaa aatgtgaaat cgaaaagcgt ttttgtagca tttcacgaca
    14401 aactggtctc tgttcctgta ggttattcag ttaacagtca ggttttattt ggtgccagat
    14461 tcattctcaa tggtgaaacc tttctggcac aggattattc taccattgaa tatttcatca
    14521 gccccatcaa aggcggaaaa attgtcggca cttactttcc caaatcagaa ggattcaatg
    14581 agttttcatc tgtttacggc aaatttatta tcagtgccga tggtaaaaac ggcagcgatt
    14641 tgtatgttgt tgaattttca aatgggtcat tctccaaacc cgaacgcctg tcgttgaata
    14701 ctgaatataa cgaattcaat ggaatcttca gcaatgatgg tgaaaaaatc tattttactt
    14761 ccgatcgccc cggcggtttt ggcggtctcg atatttacga aattgaactc atcgggaaaa
    14821 acaaatggag ccagcccaaa aatctggggc cgtccatcaa tactgctgct gatgaacaat
    14881 gcccgtttat gctcaaagac ggtatgacct tatatttcag ctcaaacggt catgtgagca
    14941 atggtgattt cgacatttac attgttacgc agggcgatga tggcgaatgg ggagatcccg
    15001 acaaattggg tgaacccatc aactccgaca aagatgatct gtactatcgc atgtcgcccg
    15061 atgaaacacg tgccgtttat tacactttcg gcgagtcggg cgaaggaata tacgaactga
    15121 ttttcaatta aattttcaac ttttttttga gtcatgtaat gaaaaagacc tggaaaaaat
    15181 ccgggtcttt ttttatatta taccaaatgg aaatataaaa tagtcaattg tagcaatttc
    15241 attgaacgtt ttgttggctg atgccagatg tttacctgac aaaacaggca ggcatcgcat
    15301 atctgcagaa aaaccaatac cgaccacaag atccgccggc tggcggatcg tatattaaca
    15361 agcattgtca gaagacgaag ctccttatat tcgactcttc cggagtctcc caaaaatatt
    15421 gaaccaattt ataaatcact ttggtattat ccaaaggaaa atctgtattt caatttattg
    15481 aaaaaagccc ggcagaaccg ggcttcagtc tttatcaaca aattctattt caatcggtcc
    15541 ttaaacggtt caaacgtcat gaaatcggca tgatgcacaa tgaacgcttc ggttgtacgc
    15601 ttcaccatgt tgccttcacc tgcatgggtg gcaataatat gacaaacatc ggcaggaaca
    15661 ccacattgct cagcaagtga aacaccacta aacggatgac gcagatattt tccatacatg
    15721 ccctgcaccg actttccatc tttcatttcg tattcaagca acttacccac atcggccaga
    15781 atagcacctg caatcaacac atccatattg accggcagat cggtacggaa aaattcacca
    15841 catttcaaac cggcatcgcg ggcaatatgt actaccgcgc gcttatgatc cataaaactt
    15901 actttcaggt cgggacctgc cagtaatgtg aaaggaatgg tgcgcagatc ttctggagta
    15961 agaacacttt tctgcagtgc cagcgcccat gtgagggtgg tcttttcttt cagttcagca
    16021 tcctttatcc aatcaagctc aggccataac tcacgcgcct gatcaacata ttttttgtca
    16081 atggcttcca taatcaaatt atttcagttt ggcaataatg gcgtcggcca tttgttttgt
    16141 tgaagcagca ccctgattaa caacatcctg agtacctttc atcttaagca tatcgtatgc
    16201 acggactttt ccctcagaaa ccacctcttc ggtagctttg cggatgcggt tggcaatttc
    16261 gttttcattc agatgttcaa gcatcagaac cgatgaaaga accattgcaa tgggattcac
    16321 aatcgatgtt tcaaaatcgg catatttcgg agctgatccg tgggttggtt caaaaacagc
    16381 cacacctgaa tcagggttga actgcgccga agtagcaaag cccaatccgc caatcaatcc
    16441 ggcaaatgcg tcagaaacaa tgtcaccgaa catattttcc gaaacgatta catgatagtt
    16501 ttcaggattt ttggtcagcc acatcatcat ggcatcgatg ttcacatctt cgacccagat
    16561 tccgggatat tcattcttac tcatttcctg agcaattttg agcatcatgc ccgaagtttc
    16621 gcgaataaca tttggttttt cacaaagagt gattttgtca aaaccgcgtt ttttggcata
    16681 ttcaaaagca gcgcggataa tgcgggtagc gaattttttg gtaataatac gggttgaaac
    16741 agccagttct tcgcgtggac accaaccaaa attgggtttg aaacgcggat gagacatcaa
    16801 agcttcatac acctgatcgt tggggttggt ccactcaatg ccgccataca aaccttctgt
    16861 gttctgacgg aaaatcattg catcaacaac gggttcatca acgccacccg ttgcattgcg
    16921 acggacgaaa ttgagcggat ttcctttcag agtctgacag ggacgcatgc aaatgtcgag
    16981 accgaatttc tgacgcatgg taacaatagg gctgtaatac accaaacctt tatcgcgcaa
    17041 ttcaggagcc aattccgctg cagctttgtc ttttggtttt gaagtgatgg ccccgaacag
    17101 accaatttta tgttcttcga gaagtttaat ggtcctgtcg ggcaacgggt ttccttcttt
    17161 acaccagaat tcccagccga tatcgccggt aacataattt gcatcaaagc cagctgcatc
    17221 aagcactctg atagattctt ccaatacaac ctgaccaatt ccgtcgccgg gcatggctac
    17281 aatagttctt tttgccatat actttttatt ttaaggattt tcgaaggcaa agaaacaaaa
    17341 aatttatatt tgcacattaa taaaaggtgg aaaaaccaga aaaaatcaga aaaagaaatc
    17401 atgaatattc ttgttttaaa atatctgatt cttattttga ttaacagtgc acaatctgta
    17461 cctgtcaagg aaaaaatgcc attcaaaccc aaaatgaatc cgccaggcac cgtttggctg
    17521 cgcgatagct tgtttattga tgtggctcct gtgaaaaatg tcgattacaa agaattcgag
    17581 tcctttgtac aaattgcata cagcaaagaa ttgcgcgata gtctgaaaaa cataccctct
    17641 tatggcattg atatgaactg ctttcagagc tatatgcggc tttgcggacc cgacaaacaa
    17701 atggcagcca aaatgaaagt accggtaaat gcaccgctaa gctgggccat gaacatgaat
    17761 gaatattaca acagtcctca ttttcggcat tatccggtac tgcatatttc gttcatacaa
    17821 gcacaggcat tctgcgaatg gcgcaccgat atggttatgc tgttgtttgc ggtttccagc
    17881 aaaaacgaaa aacaacgtac aaaatactac accagagtcc gctatcgcat gcctaccata
    17941 gaagagtggg aatatgccat ggaaaaattt aaagaaaacg tgtacaccaa catggctgtt
    18001 tatgcaggcg aacgctgcgc caccatcgaa gccgtccctc agaaaaaaaa cctggaattt
    18061 gtttatattc cgcataacat agctgaaatg accactaccg aaaaaacagc cgtcggcttg
    18121 tcatggcgtg atgcagacac aaccgacaat tattcgaaaa ccgtctctta tgccggcccg
    18181 cgcgactggc tggggttccg ctgcgtgtgc gaaattgttg aatactgata tgaaaaaact
    18241 gctgccattt attgccgtta gtctgctgtt gatttcgtgc ggacgcaaac acgaggttcc
    18301 ggataatatc aaacttttcc gctacaacga agccgccggt atccgctcac tcgacccggc
    18361 ctttgcccgc gaccaggcca atatctgggg cgtaaaccaa ctcttcaacg gtctggtgca
    18421 actcgatgat tcactgcatg tgcaaccctg cattgcaaaa tcatgggaaa tctcagacga
    18481 tggacttgtg tacacttttc atttgcgcaa cgatgtcttt tttcatcctt ccgaagcatt
    18541 caataatcaa aaacgaaaag ttattcctgc cgatgttgag tatagccttc agcgactggc
    18601 tgatgaaaaa acagcttcgc ccggaaaatg ggtgctcggg aatgtggcct gtaaatcaga
    18661 tgggaaactt gatatcatag tatcaaatga tactacaatt gctatccggc taaacaaacc
    18721 ctttgcccca ttcctcagtc tgctgtcaat gcagtattgc tcagtggtgc cgcgcgaagc
    18781 cgttgatttt tacggtaagg atttcggaca tcatccggtg ggaactgggc cgtttttgtt
    18841 caaatactgg aaagacggat tgaagttggt gttattaaaa aatccggact atttcgaaac
    18901 cgacaaatcg ggaaaacgac tcccctgcct cgatggcgtg gtggtcactt tcattgttga
    18961 aaaacataca gctttcatgg aattcatgca gggaaactac gacatgattt cgggactgga
    19021 tccaaattac aaggatgtct tgctcgatgc tgcaggaaat cttaaacagg aacacaacaa
    19081 caaaatgtac atgacgcgca ttccgtatct gaataccgaa tatctcggta tatcagttca
    19141 taaaaacggt ggcggtacca tttctgcttt gcaggaacaa aaagtgcgga tggccatcaa
    19201 ttatggtttc gaccgccgga aaatgatgcg ttatcttcgc aacaatatcg gctatcccgg
    19261 catttatggc atgattccac ccggactggc cggttatgcc gaagacagca ctttctgctt
    19321 cgagtacaat ccggcaaaat cgaagcaatt gctcgccgaa gccggatttg cggacggcaa
    19381 aggactgcct gaaattttgc tgcaaacaac agctacttat caggatctgt gcgaatacat
    19441 tcagcaacag ctggcggaaa taaatataaa agtgaaactc gacgtggtgc cacccgctac
    19501 actgcgcgaa atggtatcgc agtcaaaaag cggattcttc cgcgccagct ggattgccga
    19561 ttatcccgat gctgaaaatt acatgagtct tttttactcc aaaaacgaag tgcccaacgg
    19621 gcccaactac acccacttcg caaacactga attcgaccgg ctttacgagc aatccgtcag
    19681 tgaaccggac gcgaaaatcc gagctgacct gtatgcaaaa atgaaccgga ttctgatgga
    19741 acaatcgccg gttgtggtgc tgtattacga tatggcggtc cgctttgtca gtaaccgcat
    19801 caaaggcctg gagccgaatg cgatgaatct gctgaatctg aaatatgttg tgaaggaata
    19861 aaaaaaagtg tgattgctct cgctttcaca ctctctctaa ttcaaaatct taatcactct
    19921 ctcactcatt cccacactca ctctcgcact tgttcaaaat cttaatctca cttccgctct
    19981 cgctctcatt ctcactctgt ctttcgtggt cccaccagga cttgaacctg ggacctactg
    20041 attatgagtc agttgctcta accaactgag ctataggacc aatttgggaa tgcaaagata
    20101 aaaaaatctg ataaatactt tcaaaaaaat tagtggttcc accaggactt gaactcagta
    20161 aacacagatt atcatcgatc tgggaaaaat ttaaaatatt ttatccattg gctttttagg
    20221 attattgttt ttctgtttgc gtgcttcatt tttcttcgat ttcacataaa ccagtttgat
    20281 aaatgattta tcagtaaccc gctcgtcaat ttcaaaataa tctttgagtg atttgatcaa
    20341 aggtgtcagt ttatcaaacc catagttgcg tgggtcaaaa tccggtttct ttttaatgag
    20401 taagctgcca acatcgccca gaaatgccca ttcatcttca tcggcaatat catcaaccga
    20461 tgaccggatc aaagaaaccg tttcgtggtc aactttctga atgccatcgg gaacaagcat
    20521 tttatttttt gccaatggct gtgtttgagc cgattcaggc agtggtgctg tttttagaat
    20581 ttcaagataa ataaatttat cgcaggcaac aataaatgga tccggcgtct ttttctctcc
    20641 gaaaccaaaa actttcatgc ccgattcacg cagacgaata gccagccggg taaagtcgct
    20701 atcgctcgaa acaatgcaaa aaccatcaac acggcctgta taaagaatgt ccatggcatc
    20761 gataatcatg gccgaatccg tggcgttttt tcccgaagta tagccatatt gctgtatggg
    20821 tgtaatggca ctttcgagca acacactttt ccatccggcc agattggggc gtgtccagtc
    20881 accataaatg cgcttaaaag ttggcactcc gtatttggct atctcttcca tcagcccttt
    20941 tacattggca aacggcacat tgtcggcatc gatgatgaca gccagcttca aatcgttttt
    21001 tggttccatg aattgctttt tacaacgaca ttcttttcaa agatacggaa ttttatgatg
    21061 taattaaaaa gtctgcactg acgcggttag aaaacacata gcacggatta caaatcagcg
    21121 ctatgtgttt tggttgcagc actggcgcgg ttccgatgac tcttattaaa ctgtacactg
    21181 gcgcgattcc gataaatatc ggaatgtaca ctggtgctat aaaaaagtct gcactggcgc
    21241 ggatttgcaa ccgagacagt gtcccatttc atttcatttc ctatctttgc tcaactcaaa
    21301 aaattaattc tcatgtcggt gcgacgactt tttgttggtt tgatcatttt ctgtatttcc
    21361 gccacagcac tggcgcagac gcggtcgttg tcggcagcat tcggttatgg atttacatcg
    21421 ccacaccacc ccgacatgtg gaattacatt accggacacg cacatcaatg ccacattcag
    21481 tatctgacac cgcgcgacac aagcaatcat cggggaggtc atacattaaa agtaattgat
    21541 ctttgtttta ttaatcccgg aaaccctgat accatgggct attccatcgg gttgagtccg
    21601 cagtttcatt ttccgctgaa caaaaaacgc cgaagctttt ttgtgctggg atgcggcctc
    21661 gaatacaata ctaaaaccta taccgaagaa aattgttcct tcaatgccat cggctcgcat
    21721 ttaaatgcct tgattgtact tggctataag cacgtgtttg aactcactcc cagctggaaa
    21781 ttgaaaacgg aatttctgtt tactcatttt tcgaacggtg caaccaaagc ccccaatctg
    21841 ggtctgaatg taccaacact caacctgggt ttcgattaca gctggaatat gtcggacgaa
    21901 aaaaaatcga aaaaaaaatt acaacctgat atccaatcag gtcgctggca attcgtcggt
    21961 accggatcgt ggaagcaggt aaaacacgaa gggaaaattt caccagcttt ttgcatttcc
    22021 ggtgaaaagt ttttcccgca aacaaaaaaa tcttcatttg gttttggttc tgatttaatt
    22081 ctggattaca gcatgaataa ccggctgaca gaattcagtg ataccattgt tccgttttcg
    22141 aacaatataa aaatagctct gaaagccatg tggactgttc cgattggccg cctgcagatt
    22201 catcttcagg ccggttcgta tctgaaaaac agcaacttta aaaaagaatt tgcttttgaa
    22261 cgtctggcgc tgcgctattt tattaccgat catttcggtc tgaacgtagc tctgcgcgca
    22321 cattttgcca aagccgacgc gattgaattc ggtgctgtgt atcgtattcc cagcaaacct
    22381 gttatctttc acccgagtat cttttaactt tcaatttata tctttgcact caatgaaata
    22441 cgctgtctgc gacatagaga caacctaaaa aaataatcgt atgtgtattt tgaaaatgat
    22501 caggtattca tcaactgaac aatttctgcc ctgagtttca ttaaatcatt tgaaacaata
    22561 gcccagataa tatcttcaga aacggaatca taaccatgga taattcggtt tctggtatca
    22621 acaatttttc gcgcattcgt tatatcaatc accggattaa caatcagaat ccggttcact
    22681 gcttctccaa caatttcaat atttctctca atggcttttc tggtctttaa atcttttctg
    22741 aattcatgat aatttctttt ttccggaaga aactcataaa tttcatcaat agacttaaga
    22801 atatcttcaa gccacgtctg aatcgcgttt tccataaata aaaactttgg tgcgatcaat
    22861 ttcagattta agaaaccgat tccggattgc cttgctttcc agcaaatcta tttgcctttt
    22921 caacaaatcc tccaggttga attttaaatt gaaatacttt tctgaataag atataggatc
    22981 attatcatca atgtcaacaa tgaaatcaat atcgctttgc gcatcgaatc gttcagtgtt
    23041 taccgaacca aatgaataaa gcgatcgtac actgttcaat tcacagagta gatttatctg
    23101 atcaatatgc tgactgatga tattcatact ttacaaagat agtatttttt ttaccgttta
    23161 tcaaatgata aaaaaatcgc tctggataac aattcgaatt tactatcttt gcactcaatg
    23221 aaatacgctg tctgcgacat agagacaacc ggggggaaca atcgcgcagg aaaaattacg
    23281 gaaattgcca tttttctgtt ggaagatgga gaaattattg acgaattctc atcgctgatt
    23341 aatcccgaaa ggcccattcc gccttttatt acgcaattga ccggcattac cgatgccatg
    23401 gtggaagatg cgccgaagtt ttacgaagtc gctgcgcgca ttgtcgaaat caccaaagac
    23461 gccgtttttg ttgcacataa cgtaagcttc gattacaatt tcattgtgac cgaatttgca
    23521 cggctgggtt atgctttcca gcgcgataaa ttctgtaccc tggccaacag ccgcaagctg
    23581 attcctggcc acgcctcgta cagtctcgga aatctctgtg ccgatctgaa tattcagctt
    23641 atcgaccggc accgcgctac aggcgatgcc cgcgctaccg tcgatttatt ccgcatgctc
    23701 gatgaaatcg gaattcccga cagcccgaaa gtgctgcaga caaaactaaa acttcatccg
    23761 gcactcatgc tgacacgctt cagggaaata cctgaaacca ccggcgtgta ttattttttc
    23821 aacgataaag acgatctgat ttatataggc aaaagcacca atctgcatcg ccgtgtactt
    23881 gatcatttct acaacaaaaa agcagtgcgc gatctgcgta tgcaggaact taccgcacgc
    23941 gtcgaatacg aagaaaccgg cagcgaactc attgcactgc tgaaggaatc gcacgaaata
    24001 aaactcaaca agccattgtt caatcgtgcc ggacgccgct ctctgtctcg cttcgggtta
    24061 tatgactgcg aagacaacaa tggctatctg aatttttata tcgatgctac acagcatcag
    24121 aaaaaagcat ttccactgcg cgctttttcg gataaagact cggcactaag ctatctcgac
    24181 cgcatcatca agcgctatca cctttgtcaa aagctttgcg gaatctacga tagcgaaggt
    24241 ccgtgttttc attatcagat taaaaaatgc aggggcgcct gtgttggaaa ggaatcacct
    24301 gaagaataca acgaacgcgc ctatcaggca ctcgatgata ttcagatgaa aaatgaaact
    24361 tttctgattt cggaaggaca tgcggtgatg gataaaaaac cttttgtgtt ggttgaaaac
    24421 ggcacctata aaggatatgg ctacctgccc gaaaacgaga gtctgagcag tgttgaagaa
    24481 tgctacatgc atattcagaa acccgccatc attgataaag acgcgcattc catcattcaa
    24541 tcgtttatgc ggctgaagaa atttaaaatg gtgcggtttt aaaataaaaa atccctcgca
    24601 gtgtttaaaa cctaacgagg gattattaaa aatgaaaaac aattagtctt tcaacaattt
    24661 cacttttact gccacatctt cacctttttc atttttggca atttcgaaca taacctcatc
    24721 accgggtttc aattcagtca tgtctgtttc aacaagtgta tggaagtgaa aaaagaggtt
    24781 attgggcgga tatttgataa aaccatatcc attcttaagc gacaaaatgc ttgaagttct
    24841 ttcaatgcca tcgccctgca gttcaataaa atcttcactt tcaaaaaggt caaaatcctt
    24901 cttttcagat ctggggacaa acaatccatt gatagtggga ttggatatga aatccggatc
    24961 ttcgataagg tcgtgcattg ccaccggata agttacttcg cgaagcaaat cctgagaggt
    25021 acgggtcatt ttctgctgtc cgaaatcatc cacaaactca aaatcccagc taacaagcat
    25081 gactttggtt ccaagagcat ttacctttcg cacaagcgga acataatcgc catcactggc
    25141 aataagcacc atgacatcga aacgatgcgc cagattcaga tcataggcat ccagggcaag
    25201 ccatacatca ataccttttt ccattttaac gccttgaacg gttcggacag gaaaataatg
    25261 cgtagtgatt ccttccgaca tgagaatgtc atcaaaaaga cgatcaaaat agagctgatt
    25321 tcctttcatg ctggcctctt ttgcgccggg tcggctgcgg aaataatgag catcgacaat
    25381 ctgacaatac tgtgggtcaa tgccctcttc ctgaccaacg tactgacgaa tgaatgtgtg
    25441 aagtccacta atgctgattc tgctctgacg gttgtgcacg taattgtagt aattgctgac
    25501 atgtagaaaa taattcccgt cgtagaatac gccaatacgt gttaaatttg tcttacgatc
    25561 ctgattcata gtaatgttta ttttaaatat ttaattgcga gtgcaaatgt aaaagaatta
    25621 ttattaaatt tacaagcaga aatctgtatt ttatcatttt ccgactcaat aacactattt
    25681 taactcttgg agtatgattg aaatagtaaa gggtgacatc accaaactgg gagcagaatg
    25741 cattgtaaat gcagcaaata caagtctgct gggcggtgga ggcgttgatg gcgccattca
    25801 tcgtgcggcc ggaccacaat tgcatgaatt ctgcaaaaaa ctcaaagggg ctcaaaccgg
    25861 tgaagccaag attacaccag gtttccggct acctgccaaa tatgtgattc acacacccgg
    25921 acccatttgg cgcggaggta cacacaatga accggaactg ctgcggctct gttatttcaa
    25981 ttcaatgcaa cttgcattaa aaaacaaaat agccactctc gccttccctt ccatcagcac
    26041 cggcgtatat ggttttccat tcgacctcgc agcaagaatt gctatcagta caattcatga
    26101 ttttatcagt aaaaatcccg ctgcccccga tgtttttctg gttgcatttt cagaaagcga
    26161 tttcgatacc ctgaaaaaaa tttacagcga gatgctttag aactaaaaac cctcgcaggg
    26221 tttcaaaccc gacaagagtt cataacgcaa ttattttcta caaaactcgt tgctcattct
    26281 attaaaaatc cgtaggtttg ctttagtaaa aaacgactat gaaaaaatca cgtaaaatat
    26341 ttctcatcgc gctggcggtg atagttctgg gtatcggcgg caccgctgca tatttttatt
    26401 tcaaaaccag tcccatgcgt gccatatgga actatgttcc tcaggatgca gtgtatatca
    26461 ttgaaacaga caacctgacc aaaggatgga acaccttggc cgaaagcaac atgtggcagc
    26521 atctgataag cgatccattg tttgaggata tttcgcaaag cgcattcacc ctcgattcgc
    26581 tgatccgcgg tgatgaaacg atggatatgc tttttgccga tcgcccattg ctggttacct
    26641 gccatttact ccccaatcag gatttcgatt ttctgtttct catcgatctc aaacaagccg
    26701 gcaaatttgc ttttatcaag gattacgtag gtggcattgt tggcactttc ggctacgaaa
    26761 tgacacgtga aaaatttgaa gataccgata ttcttattat ttcggacaaa acgagtgacg
    26821 atatttttta tgtctctgtc atcgacaatg ttttcatggc ttcgtacaac aaaagtcttg
    26881 tgcaaaattc cattacatcg gccaaaacac ccgaccagtg gaaaaacaat acggcatttc
    26941 aatcggtcgt cagctccact tcgggcaata atctgttccg cttttacgta aattaccatt
    27001 tcatggctcc atacattggt tattatcttt cagatgaaca ggaattgctt gatgaactga
    27061 agcgaatttt caactactct gcattcaata tcaatctcga aagtgaaagg ttaagtttca
    27121 gcggaagcac cattctgaaa gattctgccg aaagctacat tcacatgcta agtggcattg
    27181 aacgcggccc attgcaggca taccgaatca ttccgtccga ttacgcactg tatctttctg
    27241 tttcttttga cgattatatg gatttattca ataatcttaa atcggagttt acttttaaag
    27301 attcagccgg cgccgaatca tatgaaaaaa cgctgaaaaa aatggaaaag ctttttggaa
    27361 tcaatctcga ggaagatttt ttctcgtgga tcggtaccga aatcgccatg ctgaaactgc
    27421 caccgacacc caatgcgcgc gaaaacgaca tggtagccat cattcatacc aaagacattg
    27481 aactggccat gaacggaatg gaaaaaatag taaaaaaggt aaaaaaccgc actccgatta
    27541 agattgatga ttctgaatac aagaatttca aaattcatta cttcgggatg aacggctttt
    27601 tcaaaatgtt tttcggtaaa atgttcgcaa aacttgaaaa accatactat gtttttatgg
    27661 atgattttgt tgttttcagc aattcgccca gcaatctgat ggatatgata gatgcatacg
    27721 aaaaagggcg gctgctggcc aatgatgaag agtttatgaa tttcttcgga cagtttgacg
    27781 acgaagccaa tgtatctgtt ttcttgcagg gaccgaaaat ttacagccat ctgtattatt
    27841 acgctaaaaa agatcagaaa gccaatatac gccagagtcg cgaaatactt gcatcgttcc
    27901 acaatatcgg ctttcagctc agcagcaaaa aaaagggctt tttcagcaat acactcattg
    27961 tggaacatag tgccgacgca ttgtttgctg cagaacttga agaaatagaa aacagcgccg
    28021 aagaattgta cgcaagtgaa tttgactcgc tgctagccat tgagcttcct gatgaatttg
    28081 acaatggcaa aacatgcagg atttattacg aagacgacag cacgcagatt aaagcagaag
    28141 gacgtattgt tgatggcagc cgcgaaggaa tgtggcgcta ttattacaaa agcggcgaaa
    28201 tatgggggag tttaatgttt gaagaagatg aacccattgg taaaggcata ctgtattatg
    28261 acgaagaaga tggtggaacc aaggcgcaaa ctgaatttga agatggtaaa attgaaggaa
    28321 cctatctgga atattacagc aatggtgaat caaaaaccag catcgaatat tcggaaggca
    28381 aacccgatgg tgaagcaaag ttctattacg actccggaag catcaaaatt gaaggggaat
    28441 acaaagtagg tttaaaaaag gggaaatggc gccattatac cgaaaccgga gatttgatgg
    28501 acaaggagaa atggaaaaaa gaccagaaaa aaatcaaaaa cgcaaaataa gcacacataa
    28561 tcatgttgac tttcctgatt tctgtagtgg ttcttattgc aggttatctg gtatatggcc
    28621 tgttgatgga aaaagttttc ggagttgaca aaaaccgctc tacacctgcc tttacaaaac
    28681 aggatggtgt tgattatgtg ccattaccat ggtataaaat ctatcttatt cagtttctga
    28741 atatcgcagg acttgggcct attttcggcg ctattgcagg tgccatgtgg gggccttcgg
    28801 cctttttatg gattgttttc ggctccattt tcggtggcgc tgtacatgat tatatgtcgg
    28861 gcatgatgag tctgcgcctc gatggactca gtctgccaga aatcattgga aaatatctcg
    28921 gaaaacccat gatgcaattc atgcgtgcat tcactgtcat gctgatgatt ctggtaggcg
    28981 ccgtatttat tatgggacct gccgaaattc tggcaggaat gactgcagag tctttcagta
    29041 ttgggaatct ggatatttcg ctaaacctta ctttctgggc aattgtcgtg tttgtgtatt
    29101 attttctggc taccatgctt ccgattgata aactgattgg cagaatttat ccgcttttcg
    29161 gttttgcgct gatttttatg gctcttgcaa tcatcggagc cattcttttt ggcgactatc
    29221 atattcccga aatcacaggc aatctctaca atcagcatgc aaaaggcgat gccatgccca
    29281 tttttccgat gatgttcatt tcaattgcat gcggagccat ttccggtttt catgcaacac
    29341 aaagtcctct catggcacga tgtattaaaa atgaaaaata tggtcgcagg attttttacg
    29401 gtgccatgat aacggaagga attcttgccc taatctgggc agccgctgcc atggcatttt
    29461 ttggaggcgt ggagcaatta aacaatacaa tgctggctca aaaaagcaat gccgcatggg
    29521 tcgtgaacga aataagcaac acgctgctgg ggcgctttgg ggctgtgctt gccattcttg
    29581 gtgttgtggc tgcacccatc acttccggcg ataccgcttt ccgcagcgca cggctgattg
    29641 tagccgactt tcttcatcta agccaaaaaa agatctttca ccggctcttt attgcagccc
    29701 ctttgttttt ggggggcttt gtcctgactc agattgactt tggtgtcatc tggcgttaca
    29761 tggcgtggag caatcaaaca ttggctacta ttactctatg ggccattaca gtatatctcg
    29821 tgcttgataa aaaaccctgg attgtttctt ttattcctgc cgcttttatg accatggtca
    29881 catcttctta cattttcttt gcgccggaag ggctgcagtt atcttatcat ctttctgcaa
    29941 gtatcggagc aggaattaca ctcatcgtgc tcatgctttt tttcatttgg ctcaatcatg
    30001 ctcgaaaaaa gtcagcgcca aagttatagc acctctcctt tagctgaatg catcctgttt
    30061 ttatgaagtt tcgttttttc aaggattgtt atctgtcaag tttttttcat caattgaaaa
    30121 aacttttcta atttagccac gtttaaatac ataaactgaa taaaaatgaa gaaattcatc
    30181 tttctgttac ttctcctccc ggtcgttttt cttacttcct gtgataaaaa cagccagatt
    30241 cttgtcttca agaaagatat tacctacaag gtaaaaggaa ctgccaccga aattctggta
    30301 acctatacga atgacaaagg agaaactaca ctgacaggac tttcaacagg aaccattccc
    30361 tggagttatg aattcagtgc aaaacctgat acatacgtat atcttcaggc aaaaaacgcc
    30421 accgatacag gcaccgtaga agttcagatt ctgcagcgcg atgctgtgct ggcttcagat
    30481 gaaaacgatc tgccctttgg agctgcgacc tgcagcagtt tcgtaaaata aatcagaatt
    30541 caaccttaat ggttgtacct ttttcattcg aatctatctg atatcggcca ccgagttggt
    30601 tttcggcaag catggccacc atcgataacc cgagcgactt ttctttgttt tgcaacagag
    30661 ctgccggaat acccggtccc tgatcactga tgaccaattc atgtttgctg ccctgatttt
    30721 tgaaaacaat gctcagctgc acatttccat cggagccaat accatatctg atgctgtttg
    30781 ataaaagttc ggatacaatc aatcccagcg gtaccacttc atcaattgtt gcattaagct
    30841 catcagaatc aatggtgtat ttcaccctgc tgagcgtatc aaattgctca attgaattta
    30901 taacaatggt tttcagataa tttctgaaat caatatcctt gaaatcggtg ctaaaataaa
    30961 aatcttcgtg tgcagtggca atcgccataa ttttctgaat aatatccctg aaagtctgca
    31021 gaatcttttc attatcatag gttccggcat acattttcaa catggacaaa accagctgca
    31081 gattattttt gatgcggtgg tgcacttcca taatcaagcg ctcccgctca cgaagtgcca
    31141 ttgttataaa ttcttcgtgt tttttgcgtt ctgtaatatc acgtgcgatg ccaaacacct
    31201 ctatcgggtt accgtctttg tatcgtaaaa aactattgat ttcgagaatc attttttcct
    31261 tggtgcgggt aaatgccgta acctcataag ttgagtgttc agtggcatga ctcaatttaa
    31321 gctttatctg ttcctcggct cgtttgtagc tatcgtgaga aataaaatcg ctcatgtttt
    31381 tatccttcag atcctgaaaa cgatatccaa gccatttttc agccacagga tttactgatg
    31441 tgaaattgcc ctgaaaatcc atggtgtaga taaggtcgct ggtattgttg tatatgtcaa
    31501 gaaagcgctc acgggtaatt tccagttcct gctctttcac ttttatttca gtaacatctt
    31561 cagcaataat caatacatat ttcgattttc ccgattcatc tggtatgatg ctgatcttca
    31621 ctttgaaact atgcagcacc ccatcgggaa aagcatactg atattctgta aacaatggct
    31681 cacgtgtctg cataacctgt ttaactgcat ctgcaatgcc cagtatattg gccatattgt
    31741 ttttcagaag attggtttcc tgttgcttat caatatatga caacccgaaa atcctgagaa
    31801 acgacggatt catttccaat atttcgccct caacgtcaca aatcataaat ccaataggtg
    31861 cattatcaaa aagcgatttg aatttaattt cattttcaac caccgctctc gctttattct
    31921 tcagttctgt aacatcgacc atggatccag tgatgcgctc ttcagggttg cccttgtcaa
    31981 caatggttgc agtaacaatg gtgttgaaaa cctttccgtc aggtagtctg atgtctatat
    32041 caaaattatc gacacgaccg gtggataata tttctttgac ataggcatcg cgctgcaaag
    32101 gattctggta taaagtcaga atattggttc caatcagtgt ggccggatta atattggtat
    32161 agtggtataa agaaggactg atttcaagga tggttccgtc cagtttaatt tcaaaataaa
    32221 cgttctgaat gtaattgaaa atgcgttggt atttttgttc attttttttc acagtttcca
    32281 gaatctgtaa ttctgccttt cgggaatgca gaataaaaat aaaagaagtg atggttgtgt
    32341 ctgtagcttt aaccggaata atccgcccgc tcgaatactc ggcaagtgca ggtaaattcc
    32401 tgaaaacatt ctgcgagaca tccatcaaaa cctcgctgcc ttgttttaac tgatcaatat
    32461 ctttaattcc cagcacattg atgaattcag gatcaaacaa atgcggtgat ttatacaatt
    32521 taaacaatgc gttgaaagaa ttattggctt taataagatg cccatcactc gaaaaaagac
    32581 cgaatgccag atctgttgcg tcaaagaccg aagtcatatg gcgattcaac tgatcgcatc
    32641 gctgaaaatt gaataacaaa atataaaatt gtcttgccag ccggatcatc ctgtcaatct
    32701 caatgaattt tacttcgggc aaacaatcgc acaacgccat agaaatagtt gcaatgattt
    32761 tgtttccatc actgatgggg taaccataga aataatgcag attgatgaat tcttcaacag
    32821 ccggacatgc ttcttccgga atggatttat gcacaaaatg atacaacgat tctatctgct
    32881 gcagacgatc ttcgaacatg ggagatatat catcaagact gtttttcatg tcaaaagtca
    32941 attgacctga ttcagccatt tttctgactt tatccagcag atcagagctt ccatccatcg
    33001 atacagaatg aaactccgta ttttcattgt tgcaaagcga aataacagta acagcatgtt
    33061 cctgcccgat aaccgaatat aaaaaagcag ttaaaaaatc agaaagactc tgaatatctt
    33121 taaatgtcga aaacccttca agcagttcga cataacggtc atgatgcttt ttgccaaatt
    33181 gccggagttg cactgcatcg agatgttcaa tgattttttt tgcagagaca tcggcatcac
    33241 ctgcatcttc aagaaactta taaatatctt ctactgatga attcataaaa gtataattta
    33301 ttatctttga ccaacctttg atacaagaga tacacaaagg taaaacctgt aaattaaaaa
    33361 aatgtcaata attcttaaaa acgccaacta ttacgacttc aggcaagata agatactgtt
    33421 taccaatatc ttagtagaaa gtgagagtaa aaagaaactc acaatcagtt ctgatgtcaa
    33481 tgatttgctt gataatcaga cagatgtcag aattatcgat tgttttggaa aactggtgat
    33541 gcactcattt gtcaatgcgc atcatcacag ctatgtcagt ttgaccagaa cgcacaaact
    33601 gacgaatact gacgatttcg aatttcgatt aaaaaccgga gcatggaaga tcgatcagtt
    33661 tctcgatgcc gatgttttgg aggcctgtgc cctttattcg gccatagaat cgctgaaaaa
    33721 tggtgttacc tgcgtgattg agcatcactc aagccaaaac tacatcaagg gttcactcga
    33781 aaccattgaa aaggccttta aaaaagtagg actgcaattt ttaccatgtt ttgaaatcag
    33841 cgatcgtaaa agttattcgg catcggacga agcgctggat tataccgaca gctggctttc
    33901 gggacatgca ggtctggtag gactgcatgc aagttttacc gttggatcga caacactaaa
    33961 aaatgcggtc gatatggcca ataagcataa aaccggaatt cacattcatg tggccgaaag
    34021 caccatcgat caggagcaaa gtcttaagga atacggagtt actgtagttg aacgcctata
    34081 tgatgcagga gtgcttgaaa atacaaaaac ggtgctggca catgccattc atctgagcaa
    34141 tgaagaagca gaattaatca acaaaagcgg cgtatttgta gtcgaaaaca tggaaagtaa
    34201 tttgcgcagc aatgtaggtt ttttcaattc agaaggcatt ggtgataata ttatgcttgg
    34261 aaccgatgga ctgcacggca acatgctgcg aagcgccaaa atggcatttc tggccggaca
    34321 gggcttcgac catatcgatc cctccatcgc cattcagcgt cttcgagccg gaaatgatta
    34381 ttttctcaaa aacaaaatct gtggcgatgc cgacaacaat ctgctggttt tcgattatcc
    34441 gtcgcccgaa gaagtaacac cctccaatat ggccatgcac atgatttttg gaatggaaag
    34501 caaacatatt tcgcatgtca tttcaaatgg aaatcttgtg gtcgaaaacg gccgcctgac
    34561 aatggtaaac gaagatgata ttataccttt tatcaggcaa atgacaagaa aactgatcaa
    34621 acaactcgaa aaatcaaagt aatgatgttc aaatcaatga gatttgttac gggcctggtt
    34681 ttgttccttg ccctttgttt gttttccgga aaccttgtaa atgctcagat cgtctctcct
    34741 gaagaagcaa acaccgtggc tgccaatttt gctacacaac gtcttggcaa caacttcgcc
    34801 ccttattttt cagaatttta taccgcggag tataacgggc agacaaccta ttatgttttc
    34861 aatctttttc cggagggatt tgtgattgtc tctgccaata aaaatgttgt tcccatattg
    34921 gctttttcac ccgaaaacaa ctgcccgaag aatcattcaa ttccggcgct gagtgcgttg
    34981 ctcggaaatt attcgaaaca gatttctgaa acttcagaat tgaaaaatta tcggaatccg
    35041 gaaaatcagc agctgtggtc cgattatctg cagggtgtca tcaaaagccc gaaaggacag
    35101 gttgaaccgc tgctgacaac aacctgggat cagccaacct attacaatga attctgtcct
    35161 gccgaccccg atggccccga tgacaaatgt gtgactggtt gcgttgccac agcactcgga
    35221 caattggtga attattttcg atggccatat tcaggcactg gatcctacac cagcgaagac
    35281 actgtatatg gttcgcttac agttaactat gctgctgctc attacgattt caacgaaatg
    35341 gccatagacc ttacccgcag taaccctgaa acggcagaac ttatctacaa tataggagtt
    35401 ggcgtcgata tgcactacgg ccccgacgga agcggcatga acaaccacaa ggccgcacac
    35461 gttttaaaaa catttttcaa atatgccgat tcaacccaat atattttccg ggattcggtt
    35521 tcgctcaact gggacagcgt gatcataagc caccttgaac gtggaatacc catgtactat
    35581 gcgggctggg gcgacaccat ctatatgagc ggacatgcat ttgttgtgga tggctatcag
    35641 gacacctgct attttcatat caactggggc tggggtggca gctccgatgc ctatttctac
    35701 accagcgatc tgtcacccgg atacaatttc aacctgatgc aggaattggt catcaacatg
    35761 tatcccgatg ggccgtatcc gtattactgc aacggaaccg acaccgtcag ctcgcgcgat
    35821 ggcaccatcg acgatggctc tggccctttg tttggctatc gcaacaatac cgactgttca
    35881 tggctgattg cgcccgacga cagcatcagc agcattgagc tgtcatttct gcgtttcgac
    35941 acagaaacag gtaatgatat attgacaata tatgacggtg aaaatacttc agcacctgta
    36001 cttggctcat acagcggttc ttcaatccct gcaaccattg aatcatccgg aagccgggtt
    36061 tttatgacat ttacaagcaa tggcagtgac agcgccggcg gatttctggt ttcgtacaaa
    36121 atcaatactc catacacttt ctgcgatgcc ctgacaatca ttacagctga gtctgattcc
    36181 atcgaagatg gttctggtca atatctgtat catggcaata atttctgccg atggaagatt
    36241 caaccaactt caacagaacc cgtattgctt accttttcgg aatttgatct tgattcgacc
    36301 gattatgtaa aagtaaccga tcataccagt ggtgcggtgt tgggcgaatt caaaggcaat
    36361 cagctgcctc cggttttgta ttccggcaac ggaaccatga ccattacact gaaaaccaca
    36421 gccacagcgc atgcgcaggg attcaaatgc aactatcgca catcgccggt ttcagttgct
    36481 gatatcagta aaccagatat aatgctgttt ccaaatcctg caaaagattt tattaccatc
    36541 gccggcgctg aatatgattg cgaagtgatc gttttcgatt tgcacgggcg ccgcgtatat
    36601 tctagtattc agcaagcaca aggcggttac attgtagtgc cggttcatca actgagcaat
    36661 ggcatgtatt tgctctccat tagcaataaa acgggaatac actcattgaa atttttcagg
    36721 gaatagaaaa tgaaaaacat tattgtagtt ggtgatcggg tgctgatcaa gccgaaaaaa
    36781 gaatcggaac aaacgcgttc ggggctctat ctcccacccg gctattcgca gaaagagctg
    36841 ctgcaggaag ggtatgtcat tcaggttggg cctggttatc cgatcccttc cgaagaaata
    36901 gatgagccat ggaaatcggg caccggagag aacatacgct acatcccgct gcaggcacga
    36961 caaggcgatc tggctatctt tctgcagaaa aatgcagtcg aggtcatgat cgaagatgaa
    37021 aaatatttca ttgtaccgca gaatgccatt ttgctgctgc agagagaaga ttaaatgaaa
    37081 ccttagcttt tttcagctgt aaattattga caatatttca gataaaatat tatcacgtaa
    37141 atcaacgtta ttgttggtat gaaaaaaaat atattcctga ttttaattct tctttacaca
    37201 tcggccacag cacagcatat gcgaattccg gacgggacct gggtttcgga cactgtctgc
    37261 aaaacatatc tgaatgacac acttttatgt gatgattgct gcgaaacgta tattaatccg
    37321 tttacattta ctttttcaaa cggaagactg tattgtgaca gggaaaccgg aaattccaac
    37381 agcggctatc tgatatcaga atacaagatg agcagaaata cactgcggct taagaaaacg
    37441 atgatggttg attcgtcgtt gcagattatt aatccccgaa attattacct attgtacccg
    37501 aaaaaatact tgtacgaatg tgataatgat tccgtattta ctttgattaa taaatgggac
    37561 aactgtaaat caataatcaa tgctcataaa gcacccacgc aaacgtttct gactttagta
    37621 gaaataaaaa atatcaatca gcaaagactt gattcagcaa caaccaaaaa aattgttctg
    37681 attaaaaacg attcaacttt aaaaaaggtc attctgaaaa cgaatggt
//
LOCUS       DAOPKE010000019        36149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1239_length_36149_cov_10.956419, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000019 DAOPKE010000000
VERSION     DAOPKE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1239_length_36149_cov_10.956419"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            775..2007
                     /locus_tag="PKY63_04135"
     CDS             775..2007
                     /locus_tag="PKY63_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99836.1"
                     /translation="MNKISLVVILSVISVLSSAQEKKFGLSLSGYIKTDAFYDTRQCS
                     ATNGLREGHFFLFPDNVLYDADSNDLNDNPSFNILSIQTRLTGNITGPDAFGAKTSGL
                     VETEFYGTSDADICGFRLRHGFVKLNWEKSELLIGQTWHPMFPVEAFAQTLSVNTGSP
                     FVPFSRNNQVRFAYKTGKFTTYATAWMQRDIASSGPDGTSTKYMRNSGFPGINLRTVA
                     AMSEKIQFGLGADFKTLRPEIKTAANFSTDATISSYAGFGWVKFKSEKITTSLMATYA
                     QNSTDLLMLGGYAVSEITDTLREFKTYTNLTTAAGFLDFGTNGPKWKYGVLIGFSKNL
                     GADENINGGAIYARGSNIDILYRVSPRVSCTIEKLTLGAEIEYTVAQYGTIQSSGKVT
                     NTNDVANIRALLCAIYKF"
     gene            2029..2454
                     /locus_tag="PKY63_04140"
     CDS             2029..2454
                     /locus_tag="PKY63_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE99837.1"
                     /translation="MTTTNIYLSFNGNCEETFTFYKSIFGGEYNYIGYYRDMPSKEPL
                     PAEIANKIMHVGLPIGGDTMLMGDDAAPFFGGKEVVVGTNYSIMISPDSEAEARRIFE
                     QLSVGGIIHQPLAVMFWGDLYGAFTDKFGIQWKIAFPLK"
     gene            2469..2858
                     /locus_tag="PKY63_04145"
     CDS             2469..2858
                     /locus_tag="PKY63_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE99838.1"
                     /translation="MKITETQIILYVEDQYKSRDFYKALLQADPILDVQGMTEFRLGE
                     NCKLGLMPVQGIKRLLGAAIAGLQKDKFPGAELYLMVDDAEEAVKYAEFIGAPVISEV
                     SPRDWNHRTGYVADPDGYIIAFADTRL"
     gene            2924..3916
                     /locus_tag="PKY63_04150"
     CDS             2924..3916
                     /locus_tag="PKY63_04150"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPE99839.1"
                     /translation="MITFQLDKLYDKTLMNSSAQIYLAPFQGITGQVFRTCYANHFEG
                     VDAMFTPYFAGINHDSRLSEKKKIELQNTVENGIPVIPQILSKDAGEIIRFAGILKNL
                     GFTELNWNLGCPYPQVANKKRGSGMLPYPSLVAEIMENVFAEIKINFSIKCRTGYFSP
                     DEIDELVPVFNRFPLSELIIHPRIGKQLYSGTADPEKFAHIENQLRMPVVYNGDIFTK
                     EDFIGLQQKFPSVTHWMIGRGILSDPFLPARIKGLALPADKKKQLRKFIENLYLEYRR
                     STNDSLATIGFMKEYWRYLSESFEDPHKVLKKIKKVRSFDEYEDVVAGIFSYEC"
     gene            complement(4066..5391)
                     /locus_tag="PKY63_04155"
     CDS             complement(4066..5391)
                     /locus_tag="PKY63_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPE99840.1"
                     /translation="MRFLFLVFFISFMSFCQAQTDSVAKNKKDTITGLRWAFLPVVAF
                     DSDLGLRYGGIVTAYQFGNGKLYPDYIYNFSYEFDRTNKGAISSQLFFDSGQLLRGGK
                     HRIVADFSIIHEQATDFYGFNGSASDYRKEYEDEQSENYISRMFYKFDRNILRITGDW
                     HEKLNDKWVVFGGIHHYGYKQKTVDIDKLNKGKDAGDMLPDTITLFDYYREIGVIDSA
                     EADGGNVTALRAGLVYDTRDVQFNPSKGFWLEGLIAVSPKLPWVESPFVKASVMLRNY
                     LPLYGKKLTLASRLAWQDALGKQAPFYYMSYVSQSFCNNTIAEGLGGAKTLRGVLRNR
                     IWADGFFYGNMELRWSAARFRAINQNIEIKLSPFYDFGMVTDPVVYSINQVQGLNVFI
                     DSNDTWHHGVGAGVHFIMNDNFILSVDYGFSLKPNDGDKGLYLGTGFLF"
     gene            5651..7387
                     /locus_tag="PKY63_04160"
     CDS             5651..7387
                     /locus_tag="PKY63_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99841.1"
                     /translation="MKRIFILCLIFSALSFFSAKAQNNVGIGTTSPAASALLDLTATD
                     KGLLIPRVALLAATNGTTPVASPATGLLVFNTGGAMPAGFYYWDGTQWVQVGAASAST
                     CVTLDEAYDCGGSGLGRNITVDAGRVEYNIPSGATNNEGLYIVSNKGTSATPTACTWL
                     VQNQWGVGLQVDNNLSANEYSAIQGTTFTGQTSTSFFPAGIAGYASGSGKGVGVWAEY
                     DGTNSGGAGLYAKSSGNNFAARMVGNAYPGAFIQTNSASAQAFQAASSGASYTNPCAL
                     MVGSVQFDISSNTTCQSVIFNNLAGEPTLAPEVADYGMLGTAGMYWFQGYAEAWNALS
                     RPELKRNITQIDENISALILNDIKSINPVFYKFNNEKDQFIEGSENKTRYNMHLGLLI
                     TNAPDYIQDNTFNAIDIYALSTLSLMGTKINTEQIEQVTNNVNTLSKNASISDFGFQS
                     SGESKEIRINFSADFYNQLPAGYIPVVNITPASPDAEYYIKSQDSKGFVVVSNKTRFD
                     FNWIAMAKIEIKEIEQPNASNVNNIDAEFMSQIRVAHDKKEQMKAWGLKPQQQPMQLL
                     GPSDAKETNTRN"
     gene            7409..9970
                     /locus_tag="PKY63_04165"
     CDS             7409..9970
                     /locus_tag="PKY63_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPE99842.1"
                     /translation="MKKRILFLMAMIPLLTQAQLFYNNGATVQLNPGAIVQVNGNAQN
                     QTGIINVATASAANLYITGSLTNNATINGYGNIHLNGDWINNSVFNAFTGLVSLEGAA
                     QNLSGSVVTTFYNLTLLGSGIKTQTINQTTSGTLSLNDRELATNIYTMYVTNPAINAV
                     QRTTGMVSSNNGGFLSRNTNLAAAYLFPVGSSTGTTRYRPVELTPASTAANTYVVRMA
                     NLDATSESYDRSLKEASICLVNPLFYHQINRSAGSDAVDLSIYFDQTTDGVWDSLGNW
                     HTVPTSEWYNINGSVVISGSPLSHASVTGWNDFSQLPYGLTRGIPVIDLGNDTTICSN
                     SSITLDAGSGFTSYAWNTGSTSQTISVNTGSSYSVTVTDGTCTASDIINITVVAVPLV
                     DLGADTTICQGESLILDAGTSGNGYLWSNMAVSQTITVSTSALYSVTVTNGGICPATD
                     NIQVTVQPWADATITSALNYCSGDAALNLSANDAGGIWSGTGITNGTNGTFNPGVAGA
                     GNHEIIYTIAGNCGDTDTVTINVTQSADATITPAGPFCILDAAVDLNAADAGGSWSGN
                     GITNSANGTFNPASAGIGTHTITYGISGVCGDTATTPIIVIDVADATISAAGPFCDNE
                     AALNLTAVDPAGSWSGVGITNASTGTFNPSTAGEGNHSIVYSIPGSCGDADTINITVF
                     ETPAVTVFSTNETCIGLNDGMAWVDVSGGTQPYSILWSNSETTDSIFALVPGTFSVTV
                     NDANGCGWIRQVDVEASNDLCYTPHIWVPNIFSPNGDGNNDVAFVRGDGVQYITFIIY
                     DRWGEKIFESNSLSVGWDGTYKGKALDPAVFVYYVKATFVDDSQSELHGNITLVR"
     gene            9998..11032
                     /locus_tag="PKY63_04170"
     CDS             9998..11032
                     /locus_tag="PKY63_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPE99843.1"
                     /translation="MRKIINWIFLLLFATSVSAQDIHFSQYNDAPLMINPALTGFFDG
                     SHRIALNYKNQWRSIGNAYNTYAISYDAPVTSTSAGKSGFLSMGVNVFNDVAGDLNFS
                     NLIGNLNISYHLRLSDEQLLSAGIYGGFGQRSIDQGGMQWDSQYDGTSGFDGTLPSNE
                     TFAFQTFSYADFGFGMNWSLMHNATNMTSNDGFKANAGFSVSHVNQPSFEFFGNSDEK
                     LDMRITAHAKAQIGLSGTNTSLIPTVLVQVQGSQKEIVPGLMARYTLREQSKYTGFIN
                     DAYLSLGGYFRTGDAVIAHALIELNDFAFGLSYDVNVSKLTNATSGKGGFEIMVRYII
                     QNSTKSRTLY"
     gene            complement(11425..12828)
                     /gene="ltrA"
                     /locus_tag="PKY63_04175"
     CDS             complement(11425..12828)
                     /gene="ltrA"
                     /locus_tag="PKY63_04175"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HPE99844.1"
                     /translation="MNGRKQKTEKDTCQREDRAALEDNVEGQTFLWMTENGDTSTKPQ
                     EPELLEFILSPKNLNEAYKKVMRNKGRGGVDRMQTDEIVDYLREHKDELLTQLLDGKY
                     KPHPVLRVEIPKENGKKRSLGIPVVVDRVVQQAITQQLTPIYERQFSPSSYGFRPGRS
                     AHQAVMQCLANANEGYCYVVDMDLEKFFDTVNQSKLIEVLSRTIKDGRVVSLIHKYLR
                     AGVMTHGHYEETTTGVPQGGNLSPMLSNIMLNEMDKELENREHRFVRYADDCMIFCRS
                     QRAAERVLAGITKFIESKLFLHVNREKTVVAHITEVQFLGYGFYRSKDQFRPRLHNKS
                     AEKMKAKIRLLTSRSSGWGNERRKLELKQFIIGWLNYFRLADMSKLLEKTDEWYRRRL
                     RSLIWKQWKRIKTKHRNLIKLGIPKLKAFEYANTRKSYWRTAGSPILSRSITNDRLRK
                     AGFIFFSDYYKQVHCVN"
     gene            complement(13404..13739)
                     /locus_tag="PKY63_04180"
     CDS             complement(13404..13739)
                     /locus_tag="PKY63_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE99845.1"
                     /translation="MTNWYDELPFAITVSDKEGKIIFMNNRSASTFEKYGGRDLLGKS
                     LFGCHPPQASEKIREMLESHETNAYTIDKNGQKKLIYQSPWYENGEFAGLIELSLVLP
                     ENMPHFVRK"
     gene            14303..14464
                     /locus_tag="PKY63_04185"
     CDS             14303..14464
                     /locus_tag="PKY63_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99846.1"
                     /translation="MKTTNTEKLELLEKAWNARQISESDYLMWKRVYVKNSATTTTKH
                     KRYKRQEKK"
     gene            complement(14736..15416)
                     /locus_tag="PKY63_04190"
     CDS             complement(14736..15416)
                     /locus_tag="PKY63_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPE99847.1"
                     /translation="MIKHNCKECKLKAAATCHLTNEEFQDLENNSVSVQFKKGESIIK
                     QNALSLNVAYLRKGMAKVHMQGPTRDKILRVVKAPTFLGLPTSFGEKINQFSVTAIND
                     AEVCFIDLGIFRNLIFSNGKFAYELIVELCRNELNDYFRYASLAQKQIPGLVAETLLC
                     FSEKLFDSNEFHMPLTRTEMGDLIGTSRESVSRVLSEMSQDKIIAFDSSQISILNKEA
                     LSKISEKG"
     gene            15558..16103
                     /locus_tag="PKY63_04195"
     CDS             15558..16103
                     /locus_tag="PKY63_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPE99848.1"
                     /translation="MNEEFEKQFAELQKKVGKLEKGTKDQLTMVVFSGDLDKQIAAMI
                     ISTGAAAFDMKVKLFFTFWATAALRDPKKKAKGKNFLGKMFGMMLPKGARKLKLSKMH
                     MMGMGSAMIRGLMKKKKVASLPEMFKTAGELGVEINVCEMSMDLMGFKAEELIDYPHM
                     KICGVATFLADAAESKVQLFI"
     gene            16124..16375
                     /locus_tag="PKY63_04200"
     CDS             16124..16375
                     /locus_tag="PKY63_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPE99849.1"
                     /translation="MTTEELKALTSNQTVDARGTACPGPLLAAKKAIGEIESGEIMEI
                     LSSDEGTKHDIPKWCEKMEHEFLGIVEEDSYSRLFLRKA"
     gene            16477..16926
                     /locus_tag="PKY63_04205"
     CDS             16477..16926
                     /locus_tag="PKY63_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPE99850.1"
                     /translation="MENTAFRPKILVFSTDKISDPGIDQAGLRKIHYSPSVYVISLPC
                     SSGIKPRWIMHAYEQGFDGVFIAADGHECSYSPQCAEHTNTIITETQTMMKAKNISPK
                     RIRMAAICSVCAEPFAAHMENFSKILSDLGSIEDEKNKPTDNENIAS"
     gene            16923..18272
                     /locus_tag="PKY63_04210"
     CDS             16923..18272
                     /locus_tag="PKY63_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPE99851.1"
                     /translation="MNEAIQSFDTLVIGGGIAGQEAALNLAEMNYKVVLVEKDLSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETARHHNITLKLYTEIDAIDKQNDGSFQVTVTEK
                     PRYVDDSLCTGCQECEFVCPQVINDHYNHDMVGRKAIYIPFNLANPKIAAIDIDNCIS
                     CMRCVKVCAPKAIDFKQTPKTYTMAVKSVIVSTGFKLFPATGKPDYNAASLPNVIDAL
                     QMDRVIAPTRPYNAVVRPGDGKAPENIAYVLCAGSRDSSIANKACGAGCSNNPICSQI
                     CCMYSIKQAQLLMGALPLADITIYYMDIRAFGKGYEEFYTQSKGMGVNFVKGKVAKIQ
                     SHPNGSGDVVVRYEDVHTGTYKEAKHDLVVLSVGVLPDKTTPKMFNNVKLELDEFNFV
                     RQPDPLLNPAETSIPGVFVAGMASGPKDIPDSILSAGSAAAEVASYLKIATMAQSHE"
     gene            18438..20429
                     /locus_tag="PKY63_04215"
     CDS             18438..20429
                     /locus_tag="PKY63_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPE99852.1"
                     /translation="MKERIGVYICHCGGNISDYVDVAEAQRLMQDEDGVLLAKHVMFA
                     CSDATQTEMVNDIKEHNLDAIVVASCSPKLHTHTFRGVADRAGLNKYNYIQVNIREQC
                     SWPHSDKPIDATHKAIGLIRAGIKKAALSEALESTEINATEASLVVGAGIAGMKAAIE
                     LARNGNQVYLIEKEARMGGQLRDVGTIFPTNKPGFELIDRLTQEIKSDSRITLFTETE
                     VEKVNGSIGNFSVDVHVRMAGTNTENMTLAVGSILITTGFDHYLPKENEYGFGQSDRI
                     ITLPELKKRMSASGGIVTHNGKPVRSLAFVYCVGSRQSKGENKYCSRTCCMSATHTAL
                     LLKEKNRDMKILHLYRDIRTYGKMEILYEKSSKQGDIYLKFEEKEPPVVEVNGDNVSV
                     KMKDYLTQKQEIEFQPDLLVLVTGSVPRSDSTDISSKFKIPIGADKFFNEVHPKLKPV
                     ETVINGVLIGGSCQGPKNISESVQSSLSAVSKMMSLIKDHRIQLDPVIARVNADACIW
                     CGKCAEVCEFDAIKQVEYNGKQIAEINPATCKGCGICTPICPTDALDIKLFSNAEIES
                     MIDGFMSEVKLSDGQKNEEEKPASGKKPITLKRFPEIWTRIANTMQNGHKTIPEISQE
                     LGLDTNTVTWHLMTMNKYYFVESDGMDKKDEYYYYKLKI"
     gene            20435..21514
                     /locus_tag="PKY63_04220"
     CDS             20435..21514
                     /locus_tag="PKY63_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE99853.1"
                     /translation="MATINFEFADDIKKFGANDFDACFNCGTCTAICDLSDADNSFPR
                     KMIRYTTLGITDEIKTSLDPWLCYYCGDCSTSCPRQADPGNLMMSLRRWLMSKYDWTG
                     LSGLLYRHMSAYIVAFLLIVGGIAGAYLSDVFTQEEWLHYGHIFEMVAIGAIFAVILL
                     PNLIRMWFFTVWKPTKKFNIQHHLKALGDLFLHMFTQKRSLDCEKERRDKIWYYEHLL
                     LVFSYLSLLFTTVFLDWFSTESVYVNVFGYVVSGIIFIVTTDFVIRRIRKRNEKSKFS
                     HPSDWFFVIWLLMMGISAFAVRVLIDTNLLENTFAVYLFHLTVLVQWALIIVPFGKWT
                     HFLYRSFAMYFEKIKSLTAVASKKQ"
     gene            complement(21913..22317)
                     /locus_tag="PKY63_04225"
     CDS             complement(21913..22317)
                     /locus_tag="PKY63_04225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPE99854.1"
                     /translation="MKSISLWTRNFQSVVDNGRNHSTVVDLPGAKGGDNNGPTALELC
                     VMSLSGCIGTIFAMVAQKMRLSFEKMEVELNAEQKDNAPTITDVHIVLRIQTAEDVAK
                     VEKCLETTEKTCPVGVLFTQAGVNITHEIIKL"
     gene            complement(22352..23416)
                     /locus_tag="PKY63_04230"
     CDS             complement(22352..23416)
                     /locus_tag="PKY63_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPE99855.1"
                     /translation="MKYQPNINWENYQKEIADDDYYLARSCIRQNFFPAAEDLFMKIL
                     ANDLGKNIYDDPRQTTCTGIAYHSGVIPFETTMTVVARQFALMTEAGFENFVCSCVTS
                     FGIYSEMIETWKHFPEKEKEAREILKRTTGMTFEIPKNLAHTSDIIYKFRNEIAAKAK
                     FGLINKNTNEPLKVVDHVGCHYAKIFPERGVGGAEFPYVLAGLIDAWGGAQVDYPERR
                     HCCGFGFRQYLLKSNRGYSVANSKIKFDSMQPYKPDCIIANCPGCTYFLDRWQYVIAE
                     MDGKTYGENGYGIPVLTYEELAGLLLGYNPWDIGLQVHQVAVEPLLDKLGVSYDPKAK
                     YKGPNETDMGMPEKPLHLCC"
     gene            complement(23413..24105)
                     /locus_tag="PKY63_04235"
     CDS             complement(23413..24105)
                     /locus_tag="PKY63_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE99856.1"
                     /translation="MGLYTEIKKDIRYEEGMKACINCGTCTAVCPAAAVSDYDPRQIV
                     DLVQKENEKDLEELLKKDDIWRCGECLSCKTRCPRDNVPCYIVQALRALSIETGFFMH
                     SEQGRRQLAIKRTVGDHILEYGYCVYIDEVSNEMYPEQGPVWKWMRENRTDILQRLGA
                     SYKQDKAGALRNTDKQSLADLQKIFDATGATERFRKIESASQEYAMQHGIEFSKGRDE
                     YFYKTYEGGDES"
     gene            complement(24397..24924)
                     /gene="cobO"
                     /locus_tag="PKY63_04240"
     CDS             complement(24397..24924)
                     /gene="cobO"
                     /locus_tag="PKY63_04240"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE99857.1"
                     /translation="MTADKNKGYIHLYTGNGKGKTTAAIGLAVRAAGAGKKTYIAQFV
                     KGMHYSELDSLKRFPEIEIHQFGLDCFIKNKPTQKDIDAAHTGLNCVTAIISNNQYDI
                     VVLDEICIALYYKLFPLGDVIALLEQKPDEMEIVLTGRYAPEALIDMADLVTEMKEIK
                     HYFARGIQAREGIEF"
     gene            complement(24927..25865)
                     /locus_tag="PKY63_04245"
     CDS             complement(24927..25865)
                     /locus_tag="PKY63_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE99858.1"
                     /translation="MITFGPVPSRRLGKSLGINNIPSRKVCSYSCIYCQVGLTKHFSI
                     TREVFYSPDVIFSELEKHMAALSPDDKPDYLTFVANGEPTLDLNLGKTIEKLKSFGIP
                     IAVITNASMLFDGQVREDLDRADLVSVKVDAPNEAIWRKINRPMKGLSFSNYLSGLQA
                     FKDMFHGELISETMLVEGVNNDAGSVEQTAVLVAALSPSVAYLSIPTRPPAVEWATSP
                     DEASINQAWNIFTSKKIKTELMTGFEGTFVGVTGNAIEDIVNMCSVHPIRDDVMKEIL
                     EKDKADERILSEMISLNYIICVEFDDHKYYLRKFKV"
     gene            complement(25862..26731)
                     /locus_tag="PKY63_04250"
     CDS             complement(25862..26731)
                     /locus_tag="PKY63_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE99859.1"
                     /translation="MEVAVISGKGGTGKSSVVASLASISKNILLADCDVDAANQYLLF
                     QPNHSEEEVFVSGQKAEINYDVCTQCGKCIDYCEFGAISEVNGRVEISEISCDGCRLC
                     ERICPVNAISMLDNDKSRLYSGTFRYGHMVYGRLAPGEENSGRLVSLVREKAKEIAKE
                     NNYEVILYDGPPGIGCPVISTITGIDKVIIVTEPTLSGKHDLERALETVFHFRIYAMV
                     VINKYDLNAEMTEEIKQYCATMNVPVVALLPYEPVVTEAMVNGKSYVEYAPESIVSKN
                     TLNIWKNIQNEIK"
     gene            complement(26722..27600)
                     /locus_tag="PKY63_04255"
     CDS             complement(26722..27600)
                     /locus_tag="PKY63_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99860.1"
                     /translation="MTDKIQKIKIAVASGKGGTGKTFVATNLFYAMQHANLPATIVDS
                     DAEEPNVHLFISGSLEKSFEITQKVPVIDEGMCTHCGRCHDYCTYNAIFHVPSMHVIK
                     VMEDLCHGCGACFVACNDNAISEKQSLLGTVSRYAVDATHALVEGRTKVGVYSSVKVI
                     KSSIAEADADPFVLFDSPPGTSCPFIQTANLADFVVLVTEPTPFGLSDLKQSVETLRE
                     MNKPLGVIVNRAGIGNRDVYDYLGKMNLPLLMEIPFSREIALAYSKGKLISAIDQLWL
                     EKFQDLYKTLIQQSWK"
     gene            complement(27593..28036)
                     /locus_tag="PKY63_04260"
     CDS             complement(27593..28036)
                     /locus_tag="PKY63_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPE99861.1"
                     /translation="MEKIKIGIIICNRYHTCAGGKCFRALQKREGAFSMYQNQDVEIA
                     AYTTCDGCPGGNIEYAPAEMVKNGVTHVHFATGMVVGYPPCRNIEYFEKFITEKYGLK
                     VVVGTHPIPQKYFITHQALGTWNSSSWKRLIAPTLCDEKMRLEYD"
     gene            complement(28048..28935)
                     /locus_tag="PKY63_04265"
     CDS             complement(28048..28935)
                     /locus_tag="PKY63_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPE99862.1"
                     /translation="MMDMNMTDSASTKTEKRIDERCLQCFLRTYQRLFDKFNVDQKSR
                     DEFLRFYDETWKKNDYLPAQQVQQILNTAFGKMIGEGDPFASEKKENNEIALELYQKI
                     KPQVMHSDDPFTMALRLAIAGNVMDYGAHSNFDIHATIKKALEVKLAIDYSEQLRTRI
                     AEAKHILYLGDNAGEIVFDRLFIETIMHNHVTFAVRGKAILNDVTMEDALLVGIDKVA
                     DVISNGAGNPSTVLPNCSQEFLKVFYEADLIISKGQGNLEGLIDLHDPRIFFLLMAKC
                     DVIGERLGVEKGSFNVYNP"
     gene            complement(28898..29269)
                     /locus_tag="PKY63_04270"
     CDS             complement(28898..29269)
                     /locus_tag="PKY63_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPE99863.1"
                     /translation="MKRKFAIPLENGVLCAHFGHCQTFAIVEAENNMVIDFKEVVPPE
                     HVPGLYPRWVAGFGVTDVIAGGMGQQAVMLFNEQNINAFVGAPIKSATELVEDFLNNR
                     LQLSANYCNHDGHEHDGQCKH"
     assembly_gap    29373..29472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29650..29925)
                     /locus_tag="PKY63_04275"
     CDS             complement(29650..29925)
                     /locus_tag="PKY63_04275"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPE99864.1"
                     /translation="MPGFDKTGPNGQGAMTGRRMGRCTNFGAGARNQTSEADANTNNN
                     LPDDFQANNFGWGRGGGRGFGRGFGRGFGRGFGRGFGRGAQNGNGQV"
     gene            complement(29946..30131)
                     /locus_tag="PKY63_04280"
     CDS             complement(29946..30131)
                     /locus_tag="PKY63_04280"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPE99865.1"
                     /translation="MPRLDGTGPDGNGSGTGRKLGRCSKLSDEEKLKKLGKGLGKRRK
                     ADGCGDGKGKRKKEGNQ"
     gene            complement(30144..30458)
                     /locus_tag="PKY63_04285"
     CDS             complement(30144..30458)
                     /locus_tag="PKY63_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPE99866.1"
                     /translation="MKVAITSTGDSFDSMLDSRFGRCSYFVVYDTESKSSEFIPNPNK
                     NASEGAGPASVQLLAEKNVEKIVSGEFGMKIKSLLDSLRIQMIIIKEPKSVRDVTALL
                     NH"
     gene            complement(30460..31050)
                     /locus_tag="PKY63_04290"
     CDS             complement(30460..31050)
                     /locus_tag="PKY63_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPE99867.1"
                     /translation="MARLKRLRKILNPPSMKGFKPYGQESDTEEKEPVTLLYEEYEAL
                     RLCDYDNCNHAEASAIMNVSRPTFTRIYASLRQKLANAFVECRPIVVEGGKIYFDSDW
                     HYCMKCHCNFNNPDKDRSIESCPLCGNQTFETHNSELETAFVMSGNCAEICVCPVCGE
                     KVSREPGTACNQLVCSSCGAPLNRRAGYRRGSFKNQ"
     gene            31143..31514
                     /locus_tag="PKY63_04295"
     CDS             31143..31514
                     /locus_tag="PKY63_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPE99868.1"
                     /translation="MNSATTQTRVIFPADLNNHETLFGGKALMWMDEVAYITVLRYLK
                     NNAVTIAVNKLNYINAIKAGDIIEISGVVTQVGSVKITVHVEIYREEMESGQKSKAIE
                     ADFVFAPIDENHKPVRVKMVG"
     gene            31587..32561
                     /locus_tag="PKY63_04300"
     CDS             31587..32561
                     /locus_tag="PKY63_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPE99869.1"
                     /translation="MKSFTAPSFTISARMLELVASVSEKSGAINALFLNKIPLHIQTE
                     NRFESIVSGLLLDGNNLNSKQIHDILIDSVPDWNNKENREILNLLTVYKKIGQWHPYS
                     FTSFCAAHRTLMDGLSESAGHLRTQNVGIRLYRKKIKTAPDPKHLESLLENLFSYVKT
                     SRDLLLVRSCVFFYEISFIHPFEHGNGRLARIWLKILLNEYNPAFGSLAIESLMLSQK
                     QQMHDVLSRCIRSGKSTLFIEFMLGLIDEALAILLQEKPPVISAEDRMDAFRQSFVQD
                     MFTRKEYLQFFKNISTATASRDLQTATDQGIIHKTGDKNQTVYRFRTK"
     assembly_gap    32726..32825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32826..33161
                     /locus_tag="PKY63_04305"
     CDS             <32826..33161
                     /locus_tag="PKY63_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99870.1"
                     /translation="PLFYFCNDSIGTLTYPYSMFFKAWFVSYISSYYKAENGWVTEFG
                     LFVAKVIHHNRLQDKGIHEQKLEQKLEDFENYCYSYKGFLVEDLGYIDNCTYFFFPDS
                     NRFILYYAP"
     gene            complement(33362..35632)
                     /locus_tag="PKY63_04310"
     CDS             complement(33362..35632)
                     /locus_tag="PKY63_04310"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPE99871.1"
                     /translation="MKIRILFSTLVFCFVASLSAVCSSELRLVENGKSKYCIIVRSQA
                     SQNDLIAANILQQYLFKISSAKLPIYHDNHPLAKHPAFIISQFPSGYCFETTRLLAED
                     GYCISTSESNIYITGGSGMGVIYGVTGFLEDYLGVRMLAPDAEYVPVMKVISLNPIND
                     IQIPPATIRIVNGPFASDTSYKYFRKLNTIEDRWREDDWHGYYVHTFNKLLPPENYFA
                     GHPEYFALVNGKRVPHGQLCLTNPDVLEITIEKLREEMEAHPDIQYWSVSQNDNYEYC
                     QCEKCKDVDLQEGSPAGLMLRFVNAVAKEFPDKTITTLAYQYTRKPPLITKPDSNVMI
                     TLCSIELNRTRPIAEDPGAASFREDMEGWGRISKNIMMWDYEVQFSNYLTPFPLFHTL
                     QPNLQYFTDNNVIANFQQCNASHGVEFAELKSYLMSKLMWNPDADADAVIDDFMQHYY
                     GPAAPWIRKYFDLLHAECQRYNQFLDIYGNPVQAANTYLSVENLATYHSFFDMAESLV
                     SEDTVLLARVQIARLPIMFSEMEIAKTDLFGPRGWYDKVDGDWVLRDRMIKMLEKFKQ
                     ICTQNNVIHFNENGLSFNIWYENTQRNIQVSTEDNLAFQKTVVCNPAADERYKAAGPG
                     TLTNGVHGTEDYKINWLGWEACDVEIVLDLGAVKKIQSAEISTLQYPKSWITHPVSVS
                     CQLSKDGKKYNEPVTLYSSDNLQTEPMMKAFIFDETGKKTRFIKFTITATKALPAWHA
                     YAGLKSWVFVDEIVVK"
     gene            complement(35851..>36149)
                     /locus_tag="PKY63_04315"
     CDS             complement(35851..>36149)
                     /locus_tag="PKY63_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99872.1"
                     /translation="SSCRLKSGAPQTFSADPTYVPKVKALQGKLDDLSMRLWHEALDM
                     KISIGEIIIIAGTVGIILCLIPAIKQKKALAIIGILFALGAILLGLMQGTHMFS"
BASE COUNT        10405 a         8298 c         7242 g        10004 t
ORIGIN      
        1 ttatgcacga cgcttccatg tccggcggag ttgtcgcaaa gcaaaccttg ttgccgccgc
       61 cggacgatag aaggtcgtgc ggggctgggt gcttcgggcg cgttttgaca taattgtatg
      121 tgaatgcgcg cacatgtccc ccgcgagacg aaatgccagc catttcacca aagccagact
      181 ggaagccgga tgcaattgca ctttccaaac aaaacgaaat gctggccatc tcaccaaagc
      241 cagaccggaa gccgcatttg gccacatcaa tcacgacgca ctcagatcgc ggcgcttcgc
      301 tttgcgttgc agagaggtcg tgcggggctg ggtactccgg gtgcgttttg acataattgt
      361 atgtgaatgc gcacacatgt cccccgcggg aaaatgactc gctcccaagc cgaaaaccag
      421 actggaagcc gcctgccgca gcgccgctgt cccacaggtc ctcgcgtccc accagacaat
      481 ctctgccgat ggagacagcg tatccgccag cagccgcccc gcgcgcgccc acaaagcaat
      541 tgaatatagc ttgcgttttc acaaggctaa gctacgaaaa tatttctgaa aagttcaaat
      601 gccggcgctg aggccgactt tgaggtattt tgttgaattt ctgtgtttta ccaatattgg
      661 gatgggtgtt taggatttat cttggatggt gcaacgttgg ccctggcaac acattcaaca
      721 accagcggat tgcatattaa atactatttt tgttccaaaa tcaaaaatca atctatgaat
      781 aaaatttcac tcgttgtgat tctttcagtc atttctgtac tgtcttcagc acaggaaaaa
      841 aagtttggtc tgtcgttaag cggttatata aaaaccgatg cattttatga tacacgtcaa
      901 tgctcggcta ccaatggttt gcgcgaaggt cacttttttc tttttcccga caatgtctta
      961 tacgatgccg attcaaacga cctgaacgat aatccatcat ttaatattct gagcattcaa
     1021 actcgattaa ctggcaatat cactggcccc gatgcctttg gtgcaaaaac gtcaggactc
     1081 gttgaaactg aattttatgg taccagcgat gccgatattt gtggcttccg actgcgtcat
     1141 gggtttgtga aactgaactg ggaaaaatcg gaattgctta ttggccagac atggcacccc
     1201 atgtttccgg ttgaggcatt tgcgcagaca ctgagcgtaa acaccggttc tccttttgtt
     1261 cctttcagcc gcaacaatca ggttcgcttt gcttacaaaa ccggaaaatt cactacctat
     1321 gccactgcct ggatgcagcg cgatattgca agctcaggcc ccgatggcac cagcaccaaa
     1381 tacatgcgca acagcggatt tccaggcatt aatctacgga cagttgcagc aatgagtgag
     1441 aaaattcagt tcggactggg agccgatttc aaaacattgc gccctgaaat aaaaacggca
     1501 gccaattttt ctaccgatgc caccatcagc agttatgcgg gttttggctg ggtgaaattc
     1561 aaatcggaaa aaattacgac gagtttaatg gccacttatg ctcagaacag taccgattta
     1621 ctcatgcttg gcggttatgc tgtttcggaa attactgata ctttgcgcga attcaagaca
     1681 tacaccaacc tgacaactgc tgccggattt ctcgatttcg gcaccaatgg cccgaaatgg
     1741 aaatatgggg tgctgattgg attcagcaaa aacctgggcg ctgatgaaaa catcaatggt
     1801 ggtgcaatct atgcccgggg cagtaatatt gatattcttt acagagtatc gccgcgcgtt
     1861 tcctgcacga ttgagaaact tacgctggga gccgaaattg aatatactgt tgctcagtac
     1921 gggacaatcc agtcaagcgg gaaagtgacc aacaccaatg atgtagctaa tatccgggca
     1981 ttgctctgtg ccatttacaa attctgatat cattgacctg aaaaaacaat gacaacaacc
     2041 aatatctatc tctctttcaa cggcaactgc gaagaaactt tcacgtttta taaatctata
     2101 ttcggcggcg aatacaatta tattggctat tatcgcgata tgccttcgaa agagccgctg
     2161 cctgctgaaa tagccaataa aattatgcac gtcggccttc cgattggtgg agacaccatg
     2221 ctcatgggcg acgacgcagc gccgtttttc ggcggaaaag aagttgttgt tggtaccaat
     2281 tattccatta tgatttcacc ggacagcgaa gctgaagcac gtagaatttt tgaacagctc
     2341 tcggtcggtg gcatcataca tcagccgctt gcagtcatgt tctggggcga cctgtatggc
     2401 gcttttactg ataaatttgg tattcagtgg aaaattgctt ttccattgaa ataaagtaat
     2461 tttggaaaat gaaaatcaca gaaactcaaa tcatcctcta tgtggaggac caatacaaaa
     2521 gccgcgattt ctacaaggct ttgctgcagg ccgatcccat cctcgatgta cagggaatga
     2581 ccgaattccg cctgggcgaa aactgcaaac tcggactcat gcctgtgcaa ggaatcaaac
     2641 ggctgctggg cgctgccata gccggtttac aaaaagataa atttcccgga gctgaattgt
     2701 atctcatggt cgatgacgcc gaagaagctg tgaaatatgc agaatttatc ggcgccccgg
     2761 tcatcagcga agtcagccca cgcgactgga atcaccgcac cggctacgtc gccgatcccg
     2821 atgggtacat cattgcgttt gccgacaccc gactttagtg aaaagttttt aaagttgaaa
     2881 gtttataaag acccttcgta taataactga agaacctttc aacttgataa cttttcaact
     2941 tgataaacta tacgacaaga cattaatgaa ttcttcagca caaatttact tagcaccctt
     3001 tcaaggtatt accggacagg ttttcaggac ttgttatgca aatcattttg aaggagttga
     3061 tgcaatgttt accccatatt ttgcaggcat caatcatgat tctcgactct cggaaaagaa
     3121 aaaaatcgaa ctccaaaaca ccgttgaaaa cggaattcct gtcattccgc agattctgag
     3181 taaagacgcc ggagaaatta ttcgttttgc aggaatcctc aaaaaccttg gttttacaga
     3241 gttgaactgg aatcttggct gtccgtatcc gcaggtggcc aacaagaaac gcggctccgg
     3301 aatgctgccc tacccgtctc tggtggctga aataatggaa aatgtctttg ctgaaatcaa
     3361 aataaatttc tcgataaaat gtcgcaccgg atatttctca cctgatgaaa tcgatgagct
     3421 ggtgccggtt ttcaatcgtt ttccattatc tgaactcatc attcatccgc gcatcggaaa
     3481 acaattgtat tcgggaacgg ctgatccgga aaaatttgca catatcgaaa atcagctccg
     3541 gatgccggtt gtgtacaatg gagacatttt tacgaaggaa gattttattg ggttgcaaca
     3601 aaaatttcca tcggtaactc actggatgat cggtcgcggc atattgtcgg acccctttct
     3661 gccggccaga atcaaaggac tggcattacc tgctgataaa aaaaaacaac tgcgtaaatt
     3721 tattgaaaac ctgtatctcg aataccggcg cagtaccaac gattcactgg ccactattgg
     3781 cttcatgaag gaatactggc gctacctgag tgagtctttc gaagacccgc acaaggtttt
     3841 aaagaaaatc aaaaaggtgc gaagcttcga tgagtatgaa gatgttgttg cagggatttt
     3901 ttcatatgaa tgctgattac agacagctgc cagtcctcat tcatattgtt aagattgacc
     3961 ataacttcaa cctgggcgcg cgtctccgga cgcgtgacca acttccttgc aatggcgcgg
     4021 atatgtcatc ctcccactca gctcaggccc agagctgcaa gttgttcaaa acaaaaatcc
     4081 cgtccccaga tacaaccctt tatcaccatc gttcggtttc agactgaagc cataatcaac
     4141 cgacaaaata aaattatcgt tcatgatgaa atgcacacct gcgccaaccc cgtggtgcca
     4201 cgtgtcatta ctgtctataa aaacattcaa tccctgcacc tgattgatgg aatatacaac
     4261 cggatcagtc accattccga agtcatagaa cggactcagt tttatttcaa tattttgatt
     4321 gatagccctg aagcgagctg cactccagcg aagttccata tttccgtaaa aaaatccgtc
     4381 ggcccagata cggtttcgca acacaccgcg caatgttttg gcgccgccca acccttcggc
     4441 aatggtattg ttgcagaacg attgtgaaac atatgacata taataaaacg gtgcctgctt
     4501 gcccaatgca tcctgccagg caagacgcga agccagagtc agctttttgc catacagagg
     4561 caaataattg cgcagcatga ccgatgcttt tacaaatggc gattcaaccc acggcaattt
     4621 tggactgacc gcaataagtc cttcgagcca gaatcctttt gaaggattaa actgaacatc
     4681 gcgtgtgtcg taaaccaagc cagccctgag cgcagtaaca ttccctccgt cggcttcggc
     4741 tgaatcaatc acgccgattt ctctataata atcaaacagt gtaatggtat cgggcaacat
     4801 atccccggca tctttccctt tgttcagttt gtcaatatcc acagttttct gcttatatcc
     4861 ataatgatgt attccgccga aaacaaccca tttatcattt aatttttcgt gccagtcgcc
     4921 cgtaatccgc aaaatatttc gatcgaattt ataaaacatc cggctgatat aattttccga
     4981 ctgttcatct tcatattctt ttctataatc cgatgcagag ccattgaatc cgtaaaaatc
     5041 ggtcgcctgt tcatgaataa tactaaaatc ggcaacaatc ctgtgtttac cacccctcag
     5101 caattggccg ctgtcgaaaa acaactgact gctgattgcg cccttgtttg tacgatcaaa
     5161 ttcgtaactg aaattataaa tataatcggg atacaatttc ccatttccaa actgataagc
     5221 cgtaacaata cctccatagc gcaatccaag atcggagtca aaggcaacaa ccggtaaaaa
     5281 agcccaacgc aaaccagtaa ttgtgtcttt tttatttttt gcaacactat cggtttgtgc
     5341 ctgacaaaac gacataaacg agataaaaaa aacaagaaac aaaaatctca tattccgggt
     5401 agcttttact ttacaacaat gggaaacgaa actggcagta cgatgtaagc cattcagtaa
     5461 acacatataa aaaacatttg aaacacatat aaaaatcgat taaataagca aaaaaatcaa
     5521 ccggaaaaaa tcaggtttat ttgcagataa ccaattgtta accccctttt gcagttgcaa
     5581 atgtattaat taatcgccaa caaaattgac aaagggcgca tttgtgctat cttagcaaaa
     5641 aaacaattct atgaaacgga ttttcattct ctgcctgatt ttcagcgcct tgtcattctt
     5701 ttctgccaaa gctcaaaaca atgtcggtat cggaaccacc agcccggccg catcagcact
     5761 gctcgatctg acagcaaccg acaaaggtct gctgattccc agagtggcac ttttggcagc
     5821 aaccaatgga acaacccctg ttgccagccc agctaccgga cttctggttt tcaacaccgg
     5881 aggcgccatg cccgcaggtt tttattattg ggacggaact caatgggtgc aggtgggtgc
     5941 cgccagtgcg tcaacctgtg taacactcga cgaggcctat gactgtggag gaagcggttt
     6001 aggaagaaac attaccgttg atgccggccg cgttgaatac aacattccct cgggtgcaac
     6061 aaacaatgaa ggattgtaca ttgtaagtaa taaaggtact tccgctaccc cgactgcctg
     6121 tacctggctt gttcaaaacc agtggggagt tggtttacag gttgataaca acctgagcgc
     6181 caatgaatac agtgccattc agggaacaac attcaccggt cagacttcca cttccttttt
     6241 tcctgcaggc attgccggtt atgcctcagg tagcggtaaa ggtgtcggcg tgtgggctga
     6301 atatgatggt acaaattccg gtggagcagg cctttatgcc aaatcgagcg gcaataattt
     6361 tgctgcacgc atggtcggaa atgcttatcc cggtgctttt atccaaacca actcggcttc
     6421 ggctcaggca tttcaggccg catcatccgg agcttcatac accaatccct gtgctttaat
     6481 ggtgggttcg gtgcagtttg atatcagcag caatacaacc tgccagtctg ttatttttaa
     6541 taaccttgcc ggcgaaccca ctcttgctcc cgaagtcgct gattatggca tgcttggaac
     6601 tgctggcatg tattggtttc agggatatgc tgaagcatgg aacgctcttt cacgaccaga
     6661 actgaaaaga aacataaccc agattgacga aaacatcagt gctttaatat tgaatgatat
     6721 taaaagcatc aatcctgttt tttataaatt caacaatgaa aaggatcagt ttattgaagg
     6781 ttctgaaaac aaaaccagat acaatatgca tcttggtctt ttgattacca atgcccctga
     6841 ttatattcag gacaatacgt tcaatgccat tgacatttat gctctcagca ccctttcgct
     6901 catgggaaca aaaatcaata ctgagcagat tgaacaagta accaataatg taaatacatt
     6961 gtcgaaaaat gcaagcatca gcgatttcgg atttcagtca agcggagaaa gcaaagaaat
     7021 cagaattaac ttttcagccg atttttacaa tcagttgcct gccggttata ttcctgtagt
     7081 aaatatcaca cctgcaagtc ctgacgctga atactatatc aaatcacagg atagcaaagg
     7141 atttgttgtt gtgagcaaca aaacccgctt tgattttaac tggattgcta tggccaaaat
     7201 tgaaatcaaa gaaatagaac aaccgaatgc ttcaaatgtc aataatattg atgctgaatt
     7261 tatgtcgcag atcagagttg ctcatgataa aaaagaacaa atgaaagcat ggggtctcaa
     7321 accgcagcaa cagcccatgc agctgcttgg tccaagcgat gcaaaagaaa caaacacacg
     7381 caattagtat cagtaaatat ttttcgaaat gaaaaaacga attttatttc tgatggctat
     7441 gattccgctg ctgactcagg cacagctgtt ctacaacaat ggagcaacgg ttcaattgaa
     7501 tccgggtgcc attgttcagg taaacggcaa tgctcagaat cagaccggaa tcatcaatgt
     7561 tgctacagct tcggctgcca acctttacat aaccggtagt ctcacaaaca acgctactat
     7621 caatggttat ggcaatattc atctcaatgg cgactggata aacaacagcg ttttcaatgc
     7681 tttcaccggt ctggtttctc tcgaaggtgc tgcacaaaac ctctctggaa gcgttgttac
     7741 aactttttat aatcttactt tgcttggttc gggaatcaaa acccaaacaa tcaatcaaac
     7801 aacatctggg acactgagtc tgaacgaccg cgagctggca acaaatattt atacgatgta
     7861 tgttaccaat ccagcgatca acgcagttca aagaacgacc ggaatggtga gcagtaacaa
     7921 cggtggattc ctttcccgaa acacaaacct tgctgcggca tatctctttc ctgtcggatc
     7981 aagcacagga accacccgct acaggccggt tgaactaaca ccggcttcga ctgcggccaa
     8041 tacatatgtc gttcgtatgg ccaatctcga tgcaacttcg gaaagctatg accgttcgct
     8101 caaagaagct tctatctgcc ttgtcaatcc attgttttat catcagatca atcgatccgc
     8161 cggatccgat gccgttgacc tcagtattta ttttgaccag acaaccgacg gtgtttggga
     8221 tagtcttgga aactggcaca cagtgccaac ttcagaatgg tacaacatca atggttctgt
     8281 cgttatttcc ggctcgcccc ttagccatgc cagtgttact ggttggaatg atttcagtca
     8341 gttgccttat ggactgacac gcggcattcc ggttattgat ctgggcaacg ataccaccat
     8401 ctgcagcaac agcagcatta cgcttgatgc cggttcagga ttcacttcct atgcatggaa
     8461 tactggcagc accagccaga caatttctgt aaacaccggc agctcataca gtgtaacagt
     8521 taccgatggc acttgtacag catctgacat catcaatatc actgttgttg ctgtgccgct
     8581 tgtcgatctt ggagccgata ccaccatttg tcagggcgaa tcgctgattc tcgatgccgg
     8641 cacttcaggc aatggctatt tgtggtctaa catggcagta agccaaacca ttactgtttc
     8701 tacaagtgcg ttatattcag ttactgttac caacgggggc atttgtccgg ctaccgacaa
     8761 cattcaggta accgttcagc catgggccga tgccaccatc acttcagcac tgaattattg
     8821 ttcaggtgat gcagcactca atctcagcgc caacgacgca ggcggcatct ggagcggaac
     8881 cgggataacc aatggtacga acgggacatt caatccgggc gttgccggag caggaaatca
     8941 tgagatcatt tacaccattg ccggcaactg cggtgacaca gatacagtaa ccataaacgt
     9001 cacacagagc gccgatgcaa ccattacacc agcaggacct ttctgtattc tcgatgcagc
     9061 agttgatctc aatgcggccg atgctggcgg tagctggagc ggcaatggca taacaaacag
     9121 tgccaacgga acgttcaatc cggcatcggc tggtattggt acgcatacca ttacttatgg
     9181 aatatcaggc gtatgcggcg acacagccac aacccccatc attgttattg atgtggccga
     9241 tgccaccatc agtgccgccg gtcctttctg cgacaatgag gcagccctga atctcacagc
     9301 agttgatccg gctggctcct ggagtggtgt tggaataaca aatgcctcaa cgggtacttt
     9361 caatccatca acggctggcg aaggaaacca cagcattgtt tattccattc caggttcttg
     9421 cggtgatgct gataccatta atattaccgt atttgaaacg cctgctgtaa ctgttttctc
     9481 taccaacgaa acatgcatag gtctcaacga tggaatggcc tgggtcgatg tttccggtgg
     9541 aacgcagccc tattcaattc tttggagtaa ttcggaaacc actgattcta tatttgcact
     9601 tgtaccaggc acttttagtg taacagtaaa tgatgccaac ggatgtggat ggatcagaca
     9661 ggttgatgtt gaagcttcaa acgatttgtg ctatactccg catatttggg tacccaatat
     9721 cttttcgccc aatggcgacg gcaacaatga tgtcgctttt gttcgcggcg atggagttca
     9781 atacatcact tttattattt acgaccgttg gggcgaaaaa atatttgaaa gcaattcact
     9841 cagtgttgga tgggatggaa catacaaagg caaagcgctt gatccggctg tttttgttta
     9901 ttatgtaaaa gcaacttttg tcgatgattc gcagtctgaa ttgcatggaa atattacact
     9961 tgtcagataa tttttaattt tgaacaaaat ctgcaatatg agaaaaataa tcaactggat
    10021 atttttgctg ttgtttgcca cctctgtttc ggctcaggat attcatttca gccaatataa
    10081 tgatgcgcct ttgatgatca atccggcatt gacagggttt ttcgacggat cgcaccgcat
    10141 tgctctgaat tacaaaaatc agtggcgctc cattggcaat gcatacaaca cctatgcaat
    10201 atcatacgat gcgccggtaa catctacctc ggcaggcaaa agcggattcc tgtcaatggg
    10261 agtcaatgtt tttaatgatg ttgcaggaga ccttaatttt agcaacctga ttggaaatct
    10321 caatatttca tatcatttgc gtttgagcga tgaacaattg ctgtcggccg gtatttatgg
    10381 tggtttcgga caacgatcca ttgatcaggg cggcatgcaa tgggacagtc aatacgacgg
    10441 aacttcgggt tttgatggaa cactgccaag caacgaaacc tttgcttttc aaacgttcag
    10501 ctatgccgat tttggattcg gaatgaattg gtcattgatg cataacgcca ccaacatgac
    10561 atcaaatgat ggttttaaag ctaacgccgg cttttcggtc agccatgtca atcaaccatc
    10621 gtttgagttt tttggaaatt ccgacgaaaa acttgatatg cgcatcaccg cacatgcaaa
    10681 agctcagatt ggattgagcg gaacaaatac atcgctcatc ccaacggtgc tcgttcaggt
    10741 tcagggatcc caaaaagaaa ttgtaccggg actgatggct cgttatactt tgcgcgaaca
    10801 atcgaaatac acaggtttta tcaacgatgc atatctttcg ctgggtggtt atttccgcac
    10861 cggagatgct gtgattgctc atgcgttgat tgaactcaat gattttgctt ttggactcag
    10921 ctatgatgta aatgtttcta aactcaccaa tgccaccagc ggtaaaggag ggtttgaaat
    10981 tatggtacgt tacatcattc agaattcaac caagagcaga acgctctatt agaatattga
    11041 acaaggaaca aggaaagaag atgtttgaag taaattcatt ctgattttcc agaaaatctt
    11101 aaacaagaaa gtttaaatga cgaagtgatt tgcttcgtca ttttttttag agttgtctca
    11161 caaagaaatt tactgcaaaa acaacattcc acttccgccg ctgcgggatt atgccaaaag
    11221 gaaatatgaa ataggtaaaa ataatattga caatgaacct tttgttggct gatgccagag
    11281 gtttacctga caaacaggca ggcatcacat atctatagaa aatccaataa caatcgagta
    11341 ggaggaaaat aaaacagctt taaccctgtt ttattttccg acctctcaca ccaccgtacg
    11401 taccgttcgg tatacggcgg tttcttaatt tacacagtgg acttgtttgt agtagtcact
    11461 gaagaagata aatcccgcct ttctaagacg atcattcgta atgctacggc tcaagatagg
    11521 gcttccagca gtgcgccagt agctctttct ggtgttggca tactcaaacg cctttagttt
    11581 ggggatcccg agtttaatca ggttgcggtg cttggtttta attcgtttcc attgtttcca
    11641 gatcaacgat ctcaagcggc gtctgtacca ttcgtccgtt ttttcgagta gtttactcat
    11701 gtcagcaagc cgaaagtaat tcagccatcc aatgatgaac tgttttaatt ccaacttcct
    11761 gcgttcgttt ccccacccgc tgctccgcga ggtcagaagg cggattttcg ccttcatctt
    11821 ttctgcactt ttgttgtgca gccgtggtcg gaactgatct ttcgaacggt aaaagccgta
    11881 acctaaaaac tgcacttctg ttatgtgcgc tactactgtc ttctcccgat ttacatgcaa
    11941 aaacaacttc gattcaatga actttgttat tcctgcaagc actctttcgg ctgctctctg
    12001 actgcgacag aaaatcatgc agtcatccgc gtaccttacg aaccgatgtt cacgattctc
    12061 aagttctttg tccatttcat taagcatgat gttgctcaac atcggactca gatttcctcc
    12121 ttgcggtact cccgtcgtgg tttcttcgta atgcccatgt gtcatcactc ctgcccgaag
    12181 gtatttgtgt atcagcgata caaccctacc atcttttatg gttcgtgaaa gaacttctat
    12241 cagtttgctt tggttcactg tatcaaagaa cttttccaaa tccatgtcta caacatagca
    12301 gtaaccctcg tttgcatttg ccagacattg cattacggcc tgatgggcgc tccgtccggg
    12361 tcggaacccg taactcgatg gcgagaactg gcgctcgtaa atcggtgtta gttgctgggt
    12421 aatggcttgt tgcactaccc tgtccactac cacagggatt cccaagctgc gcttctttcc
    12481 gttctctttc ggtatctcta ctcgcaagac cggatgcggc ttgtatttcc catccaaaag
    12541 ctgtgtcagc aactcatcct tgtgctcccg caggtagtca acgatttcgt ctgtctgcat
    12601 gcggtctacg cctccgcgtc ctttattccg catcactttc ttgtatgctt cattcaggtt
    12661 tttgggagac aaaatgaact ctaacaattc cggctcttgt ggctttgtgc tggtgtctcc
    12721 attttcagtc atccataaaa atgtctgccc ttcaacgtta tcttcgagtg ccgctctatc
    12781 ttcacgttga caggtgtctt tctccgtttt ctgctttctt ccattcatac agtaacgttc
    12841 atttgattct cacctcttaa gattcggccc ttcttcgctt tgcagcaaat actatggcct
    12901 ctgctgactc ctggcagcta ttgttatccg cgctattcac acgtctgtca ggcctcccgt
    12961 ggtaaacata tcccccttct gcccatgtaa ccgctgcatt tacacctcac cttccgggta
    13021 gttattggac ttcgatttgt ttggcaatct cgtccgagat gagtttgcct gatgcagttc
    13081 gtgttcctcg gttcgggctt ttgcctccag cttccttcag attccgcttc acagcggaca
    13141 cccttgctat tagctaatgg ttggtaacta ccgaccccca taaaggactt tcaccttcaa
    13201 ggtgatatgt atgcacggcg caccacaaac aatccgccgg ctggcggatt gcatattaac
    13261 aagcattatc agaatacgca gcactttatc tgcgactcct ctggagtcgt agttttgtgt
    13321 aattatggtc tacagatatg tgactcctcc ggagtcgtcc aaaattgatt tacctgatct
    13381 ataaaaaact ttggtgttag tttttatttt ctcacaaaat gcggcatgtt ttcaggcagc
    13441 acgagcgata attcaatcag gccggcaaat tctccgttct cgtaccaggg actttgataa
    13501 ataagtttct tctgaccgtt tttatcaatc gtatatgcat tggtttcatg actctccagc
    13561 atttctctga ttttctccga ggcctgcgga ggatgacacc caaataatga ttttcctaaa
    13621 agatcacgac cgccatattt ttcaaaggtt gaggctgacc ggttgttcat gaaaattatt
    13681 ttcccttcct tgtctgaaac agtgattgca aaaggtaatt catcgtacca attagtcata
    13741 atacgttatt ttggcacaaa gatacaatga ttattattgg aatttaatca aaataaaacc
    13801 tccgaaactt tcattccgga ggtgtaaact ttataaacta tcagaatcag gatataagaa
    13861 aatttcttct cttattccag atacactaag cgataatagc gttctggggt taaatcagtg
    13921 gtgtaaaatt aaacagaaaa atataaatgt caatatgatt gatgtatgtt ttttagttat
    13981 cgtatgtttt attactgtaa acgtcaatat ttaacatcaa atttattttt aatacactga
    14041 aaacatcatg ttttggcaat actttctttc tacttttcaa taatacataa aagaaaatcc
    14101 aatggctttt cagtgttttt ttttcgctaa atcttacaga tatttacggt ctttttgttg
    14161 ctattacata ctcgtgaaaa aacaatgctt aatcctaaaa caaatattaa cgtagattcc
    14221 atgaacaacc acaatattaa cattgttttg atacacgttt tgaattttta gcagtcttgt
    14281 ctttgtagaa aacaataatt atatgaaaac aacaaacaca gaaaagctcg aattacttga
    14341 aaaagcatgg aatgcaaggc aaatttcgga atcggattat ttaatgtgga aaagggttta
    14401 tgtgaagaat tctgccacaa caacgactaa acacaagcgt tacaaaagac aggaaaaaaa
    14461 atagttgctt ggccaaaaaa cttgtgtttc catttttcaa atagagattt tggcacactt
    14521 acaacattat tagaaattac gtccagaagc gacaaaactg ccccaatgaa taaaaactat
    14581 tctgtagctt acggtacgga ctggtgatat ttttatacaa ccaaaacttt attttaatgt
    14641 gctgcaaaaa ttgaattttc ctgcaaatca aacatgctgg tatagagttt atcacgttta
    14701 aagcatcgcg tttttgttaa cacttgaaaa aagattcaac ctttctccga gattttgctg
    14761 agcgcttctt tattcagaat tgaaatctgg ctggaatcga aggcaatgat tttatcctgg
    14821 ctcatttcgg acaagacccg actgacactt tcgcgcgagg tgccaatcag atcacccatt
    14881 tcagtacgtg tcaacggcat atgaaactcg ttgctatcga acaatttttc agaaaaacaa
    14941 agtaatgttt cggccaccag tcccggaatt tgtttctgtg caagactggc atatctgaaa
    15001 tagtcgttta actcgtttcg gcacaattca acaataagct cataggcaaa ttttccgttg
    15061 ctgaaaatca gattgcggaa tatgccaaga tcaataaaac atacttctgc atcgttgatg
    15121 gcagtcactg aaaactgatt tattttttca ccaaaactcg ttggcaaacc cagaaatgtg
    15181 ggtgctttaa caacccgcag gattttatca cgagtaggtc cctgcatatg caccttggcc
    15241 attcctttgc gcagataagc cacattcagc gacaatgcat tctgcttaat aatggactct
    15301 ccttttttaa actgaacgga aacactgtta ttttcaagat cctgaaactc ttcattggtc
    15361 agatgacagg tggctgcagc tttgagttta cattccttac agttatgctt tatcatggct
    15421 gatcgtattt tttgtaaata tacgaaattt aaaccaatct gtgacaaaaa tcacaatcat
    15481 gtgtagcgta tttcacacta ttgtatgaat ttcaaattaa taagtgtctt acatttgttt
    15541 attacaaaac aaccaacatg aacgaagaat ttgaaaaaca atttgcagag cttcagaaaa
    15601 aagtcggtaa gcttgaaaaa ggcaccaaag accagctgac catggtcgtt ttttcgggag
    15661 atcttgataa acagattgca gccatgatta tctccacagg cgctgcagca ttcgatatga
    15721 aagtgaaact tttctttact ttctgggcaa cagccgcttt gcgtgatccg aaaaagaaag
    15781 caaaagggaa aaatttcctt ggaaaaatgt ttggcatgat gcttcccaaa ggcgcacgaa
    15841 agcttaaact ttcaaaaatg cacatgatgg gaatgggctc agccatgatc cgcggactga
    15901 tgaaaaagaa aaaagtggct tccttgcccg aaatgttcaa aacagcggga gaactgggag
    15961 ttgaaatcaa tgtgtgtgaa atgtcaatgg atctgatggg cttcaaagct gaagagctca
    16021 ttgattatcc gcacatgaaa atctgcggtg tggctacatt cctggccgac gcagccgaaa
    16081 gcaaagttca attgttcatt taaaactaaa aaaacatact aacatgacta cagaagaatt
    16141 aaaagcactg acctcaaacc agactgttga tgcccgtggt accgcttgtc cgggcccact
    16201 gcttgctgca aagaaagcca tcggcgaaat tgaatccggc gaaattatgg aaatcctttc
    16261 ttcggatgaa ggcaccaaac acgacattcc gaaatggtgc gaaaaaatgg aacatgaatt
    16321 tctgggcatc gttgaagaag acagttattc aagacttttc ctgcgtaagg cataatatcg
    16381 tataagccca tcagggcaac gtttcatgta attgttgacg acagtttttt ttgactgtca
    16441 ttgtagaata ttgtccgaaa aaaaacattt cagcatatgg aaaacacagc tttccgacca
    16501 aaaatcctgg tcttttcaac tgataaaatt tccgatcccg gaattgatca ggcaggactg
    16561 cgcaaaatac attattcacc ctcggtttat gtgattagtt taccctgctc atcgggtatc
    16621 aaaccgcgat ggatcatgca tgcctatgaa cagggattcg acggtgtttt cattgccgcc
    16681 gacggacacg aatgctccta cagcccacag tgtgccgaac acacaaacac aataattact
    16741 gaaacacaaa caatgatgaa agcaaaaaac atcagcccca agcgcattcg catggcggcc
    16801 atctgttcgg tatgtgccga gccttttgct gcacacatgg aaaatttcag caaaatcctg
    16861 tcggaccttg gcagcattga agatgaaaaa aataaaccaa cagacaacga aaatatagca
    16921 tcatgaacga agccattcaa tcatttgata cactggtaat cggcggtggt atcgctggtc
    16981 aggaagccgc actgaatctg gctgaaatga attataaggt tgtactggtg gaaaaagatt
    17041 tgtccatcgg cggcaaaatg attcaactca gcaaggtgtt ccctaccctc gactgcgcag
    17101 catgcattac aacgcccaaa atgtcggaaa ctgctcggca tcacaatatc acactgaaat
    17161 tatacactga gatcgacgcc atcgacaaac aaaacgatgg cagctttcag gtaactgtaa
    17221 ccgaaaaacc gcgctacgtc gatgattcac tgtgcaccgg ttgtcaggaa tgtgaattcg
    17281 tttgtccaca ggttatcaac gatcattaca atcacgacat ggtcggacgc aaagccattt
    17341 acattccgtt caatctggcc aatccaaaaa ttgcggccat cgacattgac aactgcatca
    17401 gttgcatgcg ctgcgtaaaa gtctgtgcgc ccaaagccat cgatttcaaa cagacaccga
    17461 aaacatacac tatggctgta aaatcagtca ttgtcagcac gggattcaaa ttgtttcctg
    17521 ctacaggtaa gcccgattac aatgcagcat cgctgcccaa cgtgattgat gcattacaga
    17581 tggacagagt gattgcgccc acccgcccct acaacgctgt tgtcaggccc ggcgacggca
    17641 aggcacccga aaatattgct tacgtattat gtgccggctc gcgcgattca tccattgcca
    17701 acaaggcctg tggagccggt tgcagcaaca atccaatctg ttcgcaaatc tgctgcatgt
    17761 attccatcaa gcaggcacag ctgctgatgg gcgctctgcc actggccgac atcaccattt
    17821 actatatgga tatccgtgca tttggaaagg gctacgaaga attttacaca caatcgaagg
    17881 gcatgggtgt gaattttgtg aaaggaaagg ttgctaaaat tcagtctcat ccaaatggca
    17941 gcggcgatgt tgttgtgcgt tacgaagatg ttcacacggg cacctacaag gaagcaaagc
    18001 atgatttggt ggtgctttca gttggtgtgc tgcccgacaa aaccactccg aaaatgttca
    18061 ataacgtaaa attggaattg gacgaattca actttgtgag acaacccgac cccttgctca
    18121 atcctgctga aaccagcatc cctggcgtat ttgtggcagg tatggcatca ggaccgaaag
    18181 atatacccga ttcaatattg tcggctggca gcgctgctgc tgaggttgca tcgtatttaa
    18241 aaatagccac aatggcacaa agtcacgagt aatgaatgaa gccacgaagg cacgaagaca
    18301 tgagtaatga aaattggaaa caaaaacaac cacatcgaat taaagccacg aaaacatttt
    18361 tcttgtgcct tggtgtctct gtggccatct tccatgtttc tgtgtcttga tgtcttagtg
    18421 gcaaaaccca aaagcatatg aaagaaagaa taggtgtata tatttgtcat tgcggcggca
    18481 acatatccga ttatgttgat gtggccgaag cccagcgtct gatgcaagac gaggatggtg
    18541 tattgctggc caagcacgtg atgtttgcct gctccgatgc cacgcagact gaaatggtca
    18601 atgacatcaa ggaacataat ctcgatgcca ttgtggtggc ttcgtgttct ccaaagctgc
    18661 atacacacac gtttcgcgga gttgccgatc gcgctggtct gaataaatac aattacattc
    18721 aggtgaacat ccgtgaacaa tgttcgtggc cacacagcga taaacccatc gatgcaacac
    18781 acaaagcgat aggacttatc cgtgccggca tcaaaaaagc agcattgtcc gaagcgcttg
    18841 aaagcaccga aatcaacgcc accgaagcca gtctggttgt aggcgcaggc attgccggaa
    18901 tgaaagctgc catagaactc gcacgcaatg gcaaccaggt gtatctgatt gaaaaagaag
    18961 cacgcatggg aggacaactt cgagatgttg gcactatttt cccgacaaat aaaccaggtt
    19021 tcgaactcat tgaccgattg acacaggaaa taaaatcgga ttcgcgtata acacttttca
    19081 cggaaactga agttgaaaaa gtgaatggca gcatcggaaa tttttcggtt gatgtacatg
    19141 ttcgcatggc cggcacaaat actgaaaata tgacgctggc tgtagggtca attcttatta
    19201 cgacaggatt cgatcattat ttacccaaag aaaatgaata tggttttgga cagtccgaca
    19261 gaatcatcac gttgcctgaa ttaaaaaaac gcatgtctgc atcgggcggc atcgttacac
    19321 ataacggaaa acctgtgcgt tcacttgctt ttgtgtattg cgtcggtagc cgtcagagca
    19381 aaggcgaaaa caaatactgt tcgcgcactt gttgcatgtc ggccacacat acggctttgc
    19441 tgctgaaaga aaaaaacagg gacatgaaaa ttttgcatct gtaccgcgat atccgcacct
    19501 atggcaaaat ggaaattctg tatgaaaagt catcgaagca gggagatatt tatctcaaat
    19561 tcgaagaaaa agagccaccg gtggttgaag tcaatggtga caatgtatcg gtgaaaatga
    19621 aagattatct gacgcagaag caggaaattg aatttcagcc cgatttactt gtgcttgtaa
    19681 cgggctctgt gccgcgttca gacagtaccg acatttcttc aaaatttaaa attcccatcg
    19741 gtgccgataa gtttttcaac gaagtacacc cgaaactcaa accggttgaa acggttatca
    19801 atggtgtgct gatcggcggt tcgtgtcagg gtccgaaaaa catcagcgaa tcggttcaat
    19861 cgtcgctatc ggctgtatca aaaatgatgt cgctcatcaa agatcatcgc atacaactcg
    19921 atcctgtcat tgcgcgtgtg aatgccgatg cctgcatctg gtgcggcaaa tgtgccgaag
    19981 tatgtgaatt cgatgccata aaacaggttg aatataacgg aaaacaaatt gctgaaataa
    20041 atccggcaac ctgcaaggga tgcggcattt gtacaccgat ttgtccgacc gacgccctgg
    20101 atataaaact gttttcaaat gcagaaatag aatcaatgat tgatggtttt atgtcagaag
    20161 taaaactgtc tgatggtcaa aaaaatgaag aagaaaaacc tgcatcaggt aaaaaaccaa
    20221 tcacactgaa acggtttcct gaaatctgga cacgcattgc caacaccatg caaaacggac
    20281 acaaaaccat tccggaaatc tcacaggaac ttggattgga cacaaatacc gttacctggc
    20341 atctgatgac catgaacaag tattattttg tggaatcgga tggcatggat aaaaaagatg
    20401 agtactatta ttacaaatta aaaatctagc agcaatggca accatcaatt ttgaatttgc
    20461 tgatgatata aaaaaattcg gcgcgaatga tttcgatgca tgtttcaatt gcggcacctg
    20521 cacagcaatc tgtgatttat cggatgctga taactctttt ccgcgcaaaa tgattcgcta
    20581 taccacgctt ggaatcaccg atgaaataaa aacttcgctc gatccatggc tgtgttacta
    20641 ttgcggtgac tgctccacca gctgtccgcg gcaggccgat cccggtaacc tgatgatgtc
    20701 gctgcgccgc tggctgatgt ccaaatacga ctggaccgga ctttcaggtc tgttgtatcg
    20761 gcacatgagc gcctacattg tagcttttct gctcatcgtt ggaggaatcg ccggcgcata
    20821 tttgagtgat gtatttacac aggaagaatg gctgcattac ggccatatat ttgaaatggt
    20881 agccattgga gctattttcg ctgttattct gctgcccaat ctgattcgca tgtggttttt
    20941 caccgtgtgg aaacctacaa aaaaattcaa tattcagcat cacttaaagg cgctcggaga
    21001 cttatttttg catatgttca cgcaaaaacg ttcgctcgat tgtgaaaaag aacgtcgcga
    21061 caaaatctgg tactacgagc atctgctgct ggttttcagt tacctgtcat tgcttttcac
    21121 tacggtgttt ctcgattggt tcagcaccga aagcgtatat gtaaatgtgt ttggctatgt
    21181 ggttagcgga atcattttta ttgtcaccac cgactttgtt atccgccgca ttcgcaaacg
    21241 caacgaaaaa agcaagtttt cgcatccgtc ggactggttc tttgttatct ggttgctgat
    21301 gatgggcata tccgcctttg ctgtgcgcgt gctcatcgat accaatctgc ttgaaaacac
    21361 ttttgcggta tatttatttc acctcaccgt attggttcag tgggcgctca tcatcgttcc
    21421 tttcggcaaa tggacccatt ttctataccg ttcgtttgcg atgtattttg aaaagatcaa
    21481 atcgcttaca gcggtggcaa gtaagaagca gtagcaggcc ataaaggcac cagattttaa
    21541 tgccacgaag acgccaaggc acaaagattt ttactctaat aagttcccgg ccggagccag
    21601 acagaagcac taaaaatgac cggacttgcg gagcgccggg atcgcttgct acacttcaga
    21661 cttttttggc aaacagaacg gttccatatc cttcggacga tggaatgatc tgtttgcgtc
    21721 actgtcgcac ttcgaatttt agatcaacga caactaatta taaatcaata ttgtttttca
    21781 gcgaatttag caaaacagcg aaggtatatc gaaaccgcaa aagactgaaa tattctccgg
    21841 caaataatta cggtttcaaa aaaaaccggc tcagaacaag tccgagtcgg ccttagcgtt
    21901 caaaccttca agttataact tgattatttc gtgagtgata tttacaccgg cctgggtaaa
    21961 taatacgccc acagggcatg ttttttcagt ggtttcgaga cacttttcaa ctttagcaac
    22021 atcttcggcc gtctgaattc tgagtacaat atgtacatcg gtaatagtcg gagcattgtc
    22081 tttttgttcg gcgttcagtt ctacttccat tttttcgaaa gaaagacgca ttttttgtgc
    22141 aaccattgcg aagatagttc caatgcatcc gcttaatgac atcacacaca gttcaagtgc
    22201 tgtcgggccg ttgttgtcac cgcctttggc accaggaaga tcaacaacgg tgctgtgatt
    22261 acgcccgttg tcaacaacag actgaaagtt tctggtccag agagaaattg atttcatgtt
    22321 ttcttttttt agtatttggt tgtaattttt ttcaacaaca gaggtgaagc ggcttttcgg
    22381 gcattcccat atccgtttca ttcggtcctt tgtacttggc tttcggatca tagctgactc
    22441 caagcttatc caataaaggt tccacagcca cctgatgcac ctgcaaacca atgtcccatg
    22501 gattgtatcc caacaacaat ccggcaagct cttcataagt aaggaccgga attccatacc
    22561 cattctcccc atatgttttc ccatccattt cggcaatgac atattgccag cgatcgagaa
    22621 aataggtgca cccgggacaa ttggcaatta tgcaatcggg cttgtagggc tgcatggaat
    22681 caaatttgat ttttgaattg gcaacagaat aaccacgatt ggatttaagc agatactgac
    22741 gaaatccaaa cccacagcaa tggcgacgtt cggggtaatc gacctgcgcg ccgccccagg
    22801 catcaatcaa tcctgcaagc acataaggaa attcagcacc accaacacca cgttcaggaa
    22861 atatttttgc atagtgacag cccacatgat caaccacttt aagtggctca ttggtgtttt
    22921 tatttattaa tccaaacttt gcctttgcag caatttcgtt tcggaattta taaataatat
    22981 cactggtgtg tgccagattt ttcggaattt caaatgtcat tccggtcgtc cgtttcaaaa
    23041 tttcacgtgc ttctttttct ttttcaggaa aatgtttcca tgtttcaatc atttcggaat
    23101 aaataccaaa agaagtaaca caagaacaca caaaattctc aaaacctgcc tccgtcatca
    23161 gcgcaaactg acgtgccaca acagtcatgg tcgtttcgaa aggaatcaca cccgaatgat
    23221 aggcgatgcc tgtacacgtg gtctggcgcg gatcatcgta aatatttttt ccaagatcat
    23281 tggcaagaat tttcatgaaa agatcttctg ccgccggaaa aaaattctgc cggatgcaac
    23341 tgcgcgccag ataataatca tcgtcggcaa tttctttctg ataattttcc caattaatgt
    23401 ttggctgata tttcatgatt cgtcgcctcc ttcgtaggtt ttataaaaat actcatcgcg
    23461 gcctttgcta aattcaatac cgtgctgcat agcatattcc tgcgaagcac tttcaatttt
    23521 tctgaagcgc tcggtggctc ctgtggcatc aaaaatcttc tgtaaatcgg caagtgattg
    23581 tttatctgta ttacgcaatg cgcctgcttt atcttgttta taactggctc ccagtctctg
    23641 cagaatgtca gtccggtttt cgcgcatcca tttccacacc ggtccctgtt cgggatacat
    23701 ctcattactg acttcatcaa tgtaaacaca ataaccatat tcaagaatat gatcacctac
    23761 tgtgcgcttg atggccaact gtctgcgacc ttgttcacta tgcatgaaaa aaccggtttc
    23821 aatggaaaga gcacgcaatg cctgaactat atagcaaggt acattgtcgc gcgggcagcg
    23881 ggttttgcat gacaagcact caccgcaacg ccagatatca tcttttttta aaagttcttc
    23941 aaggtctttt tcattttctt tctgaaccag atcgacaatc tgccgtggat cgtaatcact
    24001 cacagccgca gccggacaaa cagcagtaca tgttccgcaa ttgatgcagg ctttcatgcc
    24061 ttcctcgtag cgaatatcct tttttatctc agtgtataga cccatattca aaattaactg
    24121 atttcaataa tacggattgc acctgttggg caaacttcaa tacattttcc acataaatca
    24181 cacaattctt cattacatac aaatcgtgta ggtttacagg gaacatccat tgtcgtgcaa
    24241 tacttcacac atttatcgca actgtaattc gacacacgac tgatggcttt tttatcacaa
    24301 atattataac aacgaccaca attggtgcac aaatgacaat caatttcgat gacctttttc
    24361 ataaccattg cttcagactc cttcatgatt ctgcaattaa aattctattc cttcccgggc
    24421 ctgtattcca cgagcaaaat aatgtttgat ttctttcatt tccgtaacca gatcggccat
    24481 atcaatcagt gcttccggag cataacgtcc tgtaagaaca atttccatct catcaggttt
    24541 ttgctcgagt aatgcaataa catcgcctaa tggaaaaagt ttgtaataaa gcgcaatgca
    24601 tatttcatca agcaccacaa tatcatattg attattgctg ataatggccg taacacaatt
    24661 caaaccggtg tgagctgcat caatatcttt ttgtgttggt ttgtttttaa tgaaacaatc
    24721 caacccaaac tgatgaattt caatttcagg aaatcttttc aacgaatcca gctctgaata
    24781 atgcatacct ttgacaaact gtgcaatata ggttttcttt cccgcacctg cagcccgcac
    24841 agcaagcccg attgcagcgg ttgtctttcc tttcccgttg cctgtatata aatgaatgta
    24901 tcctttattt ttatcagctg tcataattaa actttgaact tgcgaaggta atacttatga
    24961 tcatcaaatt caacgcaaat tatataattc agactaatca tttcagaaag aatgcgttca
    25021 tcagctttat ctttctccag gatttccttc atcacatcgt cgcggatcgg atgcaccgaa
    25081 cacatattca caatatcctc gatggcattt ccggttacac ccacaaatgt tccttcaaaa
    25141 ccagtcatca attccgtttt gatttttttg gatgtaaaaa tattccaggc ctgattgatg
    25201 ctggcttcgt cgggcgatgt agcccattca acagccggcg gacgtgtagg aatggaaaga
    25261 taggccaccg aaggcgacaa cgctgcaacc aaaacagccg tttgctccac actgccagca
    25321 tcgttgttta caccttcaac caacatggtt tcactgatca actcgccatg aaacatgtct
    25381 ttgaatgcct gcagaccact cagataattg ctgaaagaca atcccttcat cggacggttt
    25441 atctttctcc atatcgcttc gttgggtgca tcaactttta ctgaaaccag gtctgcccgg
    25501 tccaggtctt cgcgaacctg cccatcaaag agcattgatg catttgttat aacagctatc
    25561 ggaatgccga aactcttgag cttctcaatt gtttttccca aattcagatc aagtgttggt
    25621 tcaccattgg caacaaatgt cagataatcg ggcttatcgt ccggagacaa cgctgccata
    25681 tgcttttcca attctgaaaa tatcacatcc ggagaataaa acacttcgcg cgtaatgctg
    25741 aaatgctttg tgagtccaac ctgacaataa atgcaggaat acgaacagac ttttcgagat
    25801 gggatattgt ttattcccag actttttccc aaacgccgcg aaggaaccgg cccaaaagtg
    25861 atcatttgat ttcgttttga atgtttttcc agatgttcaa agtatttttc gaaacaatcg
    25921 attccggagc atattcaaca taacttttcc catttaccat ggcttcggta acaaccggtt
    25981 cgtaaggcag caaagcaacc accggaacat tcattgtagc acaatactgc tttatttctt
    26041 cagtcatttc tgcattcagg tcatacttat tgattacaac cattgcataa atgcggaaat
    26101 gaaaaacagt ttccaaagcc ctctccaaat catgctttcc cgacaatgtt ggttcggtaa
    26161 caatgattac tttatcgatg ccggttatgg tagaaatcac cggacagcca atacccggag
    26221 gtccatcata gagaatcacc tcataattat tttccttggc tatctctttg gctttttcgc
    26281 gtaccagact caccagacgc cccgaattct cttcacccgg agccagacga ccatacacca
    26341 tgtgaccata gcggaaagta ccactgtata aacggctttt atcattatca agcatcgaaa
    26401 tggcattaac cggacaaata cgctcacaaa gccggcaccc atcacaactg atttccgata
    26461 tttcgacccg gccattcact tcggaaatgg ctccaaactc acaataatca atgcatttcc
    26521 cacactgcgt gcatacatca taattgattt ctgccttttg tccggaaaca aaaacttctt
    26581 cttcggaatg attgggctga aaaagcaaat actgattggc cgcatccaca tcgcaatcag
    26641 caagtaaaat atttttcgat atacttgcca gtgaagcaac cacactactc tttccggttc
    26701 cacctttacc actgattact gctacttcca tgactgttga atcaaagttt tatataaatc
    26761 ctgaaatttt tccaaccaca attgatcgat ggccgaaatc agttttcctt tggaatatgc
    26821 cagcgcaatt tcgcgcgaaa aaggaatctc cataagcaac ggcaaattca tttttccgag
    26881 ataatcatac acatcgcggt tgccaatacc agcacggttt acaataacac caagcggctt
    26941 attcatctca cgcaaagtct caaccgattg cttcagatcg ctcagaccaa aaggcgttgg
    27001 ttcggtaacc agcacaacaa aatcggccag attagcagtc tgtataaaag gacacgacgt
    27061 tcccggcgga gaatcgaata aaacaaaagg atcggcatcg gcttctgcaa ttgatgattt
    27121 gatcactttt acagaagaat acacgccgac ttttgtgcga ccttctacca atgcatgagt
    27181 tgcatctaca gcgtaacgcg aaaccgttcc cagcagactt tgcttttcgc tgatggcatt
    27241 gtcgttacaa gctacaaaac aagcaccaca gccatgacaa agatcttcca taaccttaat
    27301 gacatgcatt gaaggaacat ggaaaatggc attataggtg caataatcat gacagcgtcc
    27361 acaatgggta cacatgcctt catcaatcac cggaaccttt tgggttattt caaacgattt
    27421 ttccaaagac ccgctgataa acaaatgcac attgggttct tcggcatcac tatcaacaat
    27481 ggtagccggc agattggcat gctgcatggc ataaaacaga ttggtagcaa caaatgtttt
    27541 gccggtacca cccttgccac tggcaacagc gattttaatt ttttgaattt tatcagtcat
    27601 attccaatcg catcttttca tcacacaaag tgggcgctat cagccgtttc cacgaactgc
    27661 tgttccatgt gcccaaagcc tgatgagtaa taaaatattt ctgaggaatc ggatgagttc
    27721 cgacaaccac tttcaatcca tatttctcag taatgaattt ctcaaaatat tcaatgttgc
    27781 ggcaaggcgg atagccaacc accataccag ttgcaaaatg aacatgcgtt acaccatttt
    27841 tcaccatttc ggcaggcgca tattcaatgt tcccgccagg acaaccgtcg caggtagtgt
    27901 aagccgcaat ttcaacgtcc tgattctggt acattgaaaa ggccccttcg cgcttttgca
    27961 gagctctgaa acattttcca ccagcacagg tatgataacg gttacaaatg ataatgccaa
    28021 tcttaatctt ttccatcctt acatttttta aggattatac acattaaaac tccctttttc
    28081 aacacccaga cgttcaccaa tcacatcgca tttggccatc aataaaaaga aaatacgcgg
    28141 atcgtgcaga tcaatcaatc cttcgagatt tccctgacct ttcgaaataa tcagatcagc
    28201 ttcgtaaaaa acttttaaaa actcttggct gcaatttggt aaaaccgtgg aaggattgcc
    28261 cgctccatta gaaatgacat cggccacctt gtcgattccc accaaaagag catcttccat
    28321 ggttacatcg ttgagaatgg ctttgcctct caccgcaaaa gtaacatggt tgtgcatgat
    28381 ggtttcgatg aacaatcggt caaacactat ttcgccggca ttatcaccca gatacaatat
    28441 atgttttgct tcggcaatac gggttctgag ctgttccgaa taatcaatgg ccagcttgac
    28501 ttccaatgct tttttaatgg tagcatgaat gtcgaaattg ctgtgagcgc cataatccat
    28561 tacatttccg gcaatagcca accgcaaagc catcgtaaaa ggatcatcgg agtgcatgac
    28621 ctgcggttta attttctgat agagctcaag cgcaatctca ttgttttctt ttttttctga
    28681 agcaaaagga tcgccctctc caatcatttt tccgaaagct gtattgagaa tctgctgcac
    28741 ctgttgtgca ggcaaataat catttttttt ccaggtttca tcgtaaaacc ggagaaattc
    28801 atcgcgcgat ttttgatcaa cattaaactt gtcaaacaaa cgttgatatg ttctcagaaa
    28861 acattgcaaa catctttcat cgatgcgctt ttccgtttta gtgcttgcac tgtccgtcat
    28921 gttcatgtcc atcatgattg caataattgg cactcaactg aagccggttg ttcaggaaat
    28981 cctcgaccag ttctgtagcg cttttgatgg gtgctccgac aaaagcatta atattctgct
    29041 cgttgaacaa catgacggct tgttgtccca tgccaccagc aatgacgtcg gtaacaccaa
    29101 aaccagcaac ccagcgtggg tacaaacctg gaacatgttc cggcgggaca acttctttaa
    29161 aatcaatgac catgttgttt tcagcttcaa caatagcaaa agtctgacaa tgaccaaaat
    29221 gtgcacatag cactccattt tcaaggggaa tagcaaattt tcttttcatt caatcttatt
    29281 tttaaaaaaa gggaggcgaa gagttacctc ccttaaaatc aaaaaccact tatgaaacaa
    29341 gcccgacctc ttctgccgaa gcattttttg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnatcaaaaa ccacttatga aacaagcccg acctcttctg ccgaagcatt
    29521 ttttgtctga atttgatctg aacctcaaca tccaattgtt taattattgt ccgatttcaa
    29581 ttaaaatgtt gttcagattt ttgtaaacca agttatgttt tttcagaacc aaaaacaggt
    29641 ttcaaaaaac tacacctgcc catttccatt ctgagcacca cggccaaaac cgcgaccaaa
    29701 tccacgacca aaacctcggc caaatccgcg gccaaagcca cgtccgccac cacgacccca
    29761 gccaaaatta tttgcctgaa aatcgtcggg taaattattg ttggtgtttg catcagcttc
    29821 agaagtctga ttgcgggcgc cagcaccgaa atttgtgcaa cgtcccattc tgcggccagt
    29881 catggcccct tgtccgtttg gacctgtttt atcaaatcct ggcatattaa cctcctgttt
    29941 ttaaattatt ggttaccttc tttttttctt tttcctttac catcgccgca accatcagct
    30001 ttacgacgtt ttccgagtcc tttccccagt ttcttcagct tttcttcatc cgaaagctta
    30061 ctgcaccggc caagtttgcg gccagtgcct gaaccattac catcgggtcc tgttccatct
    30121 aaacgaggca tgacatttta attttaatga ttcaacaatg ctgtaacgtc ccttacagac
    30181 ttgggttctt ttataatgat catctgaatt ctcagactat caagcaacga ttttattttc
    30241 attccgaatt caccggaaac aatcttttcc acattttttt cggccaaaag ctgaactgaa
    30301 gccgggcctg caccttccga tgcattttta ttcggattgg gaataaattc cgatgacttg
    30361 ctttcagtgt cataaacaac aaaatacgaa caacgcccaa aacggctgtc caacattgag
    30421 tcaaaagaat ctccggtcga tgtgattgct actttcatat tattggtttt taaaacttcc
    30481 tctgcgatat cctgcccgac gatttaatgg agcgccgcag gaagagcaaa ctaattgatt
    30541 acacgccgtt cctggttcgc ggctgacttt ttcgccacaa acaggacaaa cacaaatttc
    30601 ggcacaattg ccactcatta caaatgccgt ctccaattca gaattgtggg tctcaaatgt
    30661 ctgattgcca cacaaaggac aactttcaat ggatcggtct ttgtctggat tattgaaatt
    30721 acaatgacat ttcatgcaat aatgccaatc gctgtcaaaa taaattttac ctccttcaac
    30781 cactatggga cggcattcaa caaaagcatt ggccaacttc tggcgcaacg atgcataaat
    30841 ccgggtgaaa gttggccgcg atacattcat aatggccgat gcttcggcat gattgcaatt
    30901 gtcataatca cacaatctca atgcttcata ttcctcatac aacaacgtta ccggttcttt
    30961 ttcctccgtg tcggattctt ggccataggg cttaaatcct ttcattgaag ggggatttag
    31021 aattttacga agtcgtttca atcgtgccat aattttattt tgaacaattg ttcactgcaa
    31081 agataatgaa cttttgttca tttgggatat ttttttttac tttgtttaaa attttaacac
    31141 taatgaattc agcaacaacc caaacccgtg taattttccc tgccgacctc aataatcatg
    31201 aaaccttgtt tggaggaaaa gcactcatgt ggatggacga agtggcctac ataacagtgc
    31261 tcagatacct taaaaataat gcagtaacca ttgccgtaaa caaactcaat tatattaatg
    31321 cgataaaagc cggcgatatt atcgagattt caggtgttgt aactcaggtt ggcagcgtga
    31381 aaatcactgt tcacgtcgaa atttaccgcg aagaaatgga atcgggacag aaatccaaag
    31441 ccattgaagc cgattttgtt tttgcaccga ttgatgaaaa ccataaacca gtgagagtga
    31501 aaatggtcgg atgaacgaaa caactttaca gattcacgga tatgcaaaaa aacactacct
    31561 ttgatactat caaatgatac tatacaatga agtcattcac agccccatca ttcaccatta
    31621 gcgcacgaat gctcgaactg gtggcttcgg tgtcggaaaa gtcgggagct ataaacgccc
    31681 tgtttctcaa caaaattcca ctccatatcc aaaccgaaaa ccgatttgaa agcattgttt
    31741 cgggtcttct gctcgatggc aacaatctaa acagcaaaca aatacacgac attctgatag
    31801 atagtgttcc tgactggaac aacaaagaaa acagagaaat actcaatttg cttacggtat
    31861 ataaaaaaat aggccaatgg cacccataca gcttcacctc gttttgcgct gctcaccgca
    31921 ccctgatgga tggactgagc gaatcagcag gacatttgcg gactcaaaat gtgggcatca
    31981 gactttatcg aaaaaaaatc aagacagccc cggatcccaa acatctcgaa tcattgctcg
    32041 aaaatttatt ttcttatgta aaaacaagcc gagatctttt attggtgcga agttgcgtgt
    32101 ttttttatga aatatcattt attcaccctt ttgaacatgg caacggtcgc cttgcccgga
    32161 tatggctgaa aattctgctc aacgaataca atcctgcttt tggctcttta gccatcgaat
    32221 cgctcatgct atcacaaaaa caacaaatgc acgatgtatt gtcacgttgc atccgttccg
    32281 gaaaatcgac tttgtttatt gaatttatgc ttggactcat tgatgaagcc cttgctatac
    32341 tgcttcagga aaaacctccg gtcatttcgg ccgaagaccg catggatgct tttcgtcaga
    32401 gctttgtgca ggacatgttt acccgcaagg aatacctcca gttttttaaa aatatttcca
    32461 ccgccaccgc cagccgcgat ctgcaaacag ccaccgacca gggcatcatt cacaaaaccg
    32521 gcgataaaaa ccaaacggtg tatcggttca gaacaaaata aatatgaaaa atactgtagt
    32581 cattttatgc atttcgatat tggtgctttg tgcttgcacc aatgaaaaca aacaatcgga
    32641 caaaactttt gacgatttct cagtacatga ggatattgaa aaagctgatt cgttttgtac
    32701 cattccgtac aacggcctga aaaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32821 nnnnncctct gttctatttt tgcaacgaca gcatcggcac actgacttat ccgtattcga
    32881 tgttttttaa agcgtggttt gtgagttaca tttcatcata ctataaagca gaaaatggtt
    32941 gggtaaccga atttggtttg tttgtagcaa aagtaattca tcacaacaga cttcaggata
    33001 aagggatcca tgaacagaaa ctggaacaaa aactagagga ttttgaaaac tactgttaca
    33061 gctataaagg atttctggtt gaagatttgg gttatattga taattgcacc tatttcttct
    33121 ttcccgacag caatcgtttt attttgtact atgcaccctg atgaaaactt gtagatacta
    33181 attcaaacac acaaataaat ttaaaaccat tcacagctca ccgatcactg ctggaataat
    33241 agcattttga cttccactga ctactgactc cgccgcggcg gatggctact gaccactggc
    33301 tacaccgacg cttcggtcgg cgctttcagc tggctactga ccactggcta ctggctgctg
    33361 gctacttcac aacgatttca tccacaaaca cccacgactt caaacccgca tacgcatgcc
    33421 aggcagggag cgcttttgtg gcagtgatgg tgaattttat aaatcgtgtt tttttgcctg
    33481 tttcatcaaa gataaatgct ttcatcatcg gctcggtttg cagattgtcc gatgaataca
    33541 gcgttacagg ctcattgtac tttttgccgt cttttgacaa ctggcacgaa acgctcaccg
    33601 gatgagtgat ccatgacttc ggatattgca aagtgctgat ttcggccgat tgaattttct
    33661 tcactgcacc tagatccagc acaatttcaa catcgcaggc ttcccagccc agccagttga
    33721 ttttataatc ttcggtgcca tgaactccgt tggtcagcgt gccggggcct gcagccttgt
    33781 aacgctcatc ggctgccgga ttacatacaa ctgttttctg aaatgccaga ttgtcttcgg
    33841 tactgacctg aatattccgt tgcgtatttt cgtaccagat gttgaatgag agcccgtttt
    33901 cattgaaatg aatcacatta ttctgcgtac aaatctgttt gaatttttcg agcattttga
    33961 tcattcgatc gcgcagaacc cagtcgccgt caactttatc gtaccacccg cgcggaccga
    34021 ataaatcggt tttggcaatt tccatttccg aaaacataat gggcagccgt gcaatctgta
    34081 cccgtgcaag cagcactgtg tcctcactta caagactttc ggccatgtcg aaaaaagaat
    34141 ggtatgtggc cagattttcg accgaaagat acgtgttggc cgcctgtacc gggttgccat
    34201 aaatgtcgag gaactgattg tagcgctgac attcggcatg aagtaaatcg aagtatttcc
    34261 ggatccatgg cgcagcagga ccataataat gctgcataaa atcgtcgatg acagcatctg
    34321 catcagcatc aggattccac atcagcttgc tcatgagata ggatttgagc tcagcaaatt
    34381 caaccccatg cgatgcatta cattgctgga aattggcaat cacattattg tctgtgaaat
    34441 actgaaggtt tggctgcaaa gtatgaaaca acggaaacgg cgtcagataa ttcgaaaact
    34501 gaacttcgta atcccacatc atgatgtttt tactgattct accccaccct tccatatcct
    34561 cgcggaacga ggctgcaccc gggtcttcag caatcggtcg ggtacggttt agttcaatcg
    34621 agcacaacgt aatcatgaca ttgctgtcgg gttttgtgat cagtggcggt tttcgggtgt
    34681 actgatacgc taatgtggta atggttttat ccggaaattc tttggccacc gcattcacaa
    34741 aacgcagcat caatccggcc ggagagcctt cctgaagatc gacatccttg catttttcgc
    34801 attgacaata ttcgtaatta tcgttctgac tcaccgacca gtattgaata tcgggatgtg
    34861 cttccatttc ttcacggagc ttttcaatgg tgatttccag tacgtccgga ttagtcagac
    34921 acagctgtcc atgcggtaca cgcttaccat tcaccagcgc aaaatattcc ggatgtccgg
    34981 caaaataatt ttcaggtggt aacagtttat taaatgtatg tacgtagtat ccatgccaat
    35041 catcttcgcg ccagcggtcc tcaatggtat tcagttttct gaaatattta tagcttgtat
    35101 cagatgcaaa cggaccattc acaatacgga tggtggccgg aggaatctga atatcgttga
    35161 tcggattcaa tgaaatgact ttcataaccg gtacgtattc ggcatcggga gccagcatgc
    35221 gtacacccag ataatcttcg agaaaaccgg tcacgccata aataacgccc atgccactgc
    35281 caccggtgat atagatattg ctttcagaag tcgagatgca ataaccgtct tcggcaagca
    35341 aacgagttgt ttcaaaacaa taacccgaag gaaactgact gataataaaa gccggatgct
    35401 ttgccaaagg atgattgtcg tgatagatcg gcaatttggc cgaactgatt ttaaacaaat
    35461 actgctgcag gatattggca gcaatcaggt cattttggct ggcctgagac cgaacgataa
    35521 tgcaatattt acttttccca ttttcaacca agcgaagttc actgctgcaa acagctgata
    35581 atgaagcaac aaaacaaaaa acaagtgttg aaaaaagaat tctgattttc attctccaaa
    35641 gaaaacaaga aacatgcatt tataagctat tcaaaaccct atagcttttc atttattatc
    35701 aacaataaat gaacatgaat tcaacagaaa aacaacactt atcaacatgt tttcaaacaa
    35761 ataaaagcat caaaaagaaa ggatgggaca aatataatac tagtttacaa gcaaatagct
    35821 gtaaaaccaa ccgaagtttt gttaataact ctacgaaaac atatgggtac cctgcatgag
    35881 cccaagaagt attgcgccca aagcaaagag aataccaatg atagccaaag ctttcttctg
    35941 ttttatggca ggaataagac aaagaataat tccaacggta cctgcaataa taatgatttc
    36001 gccaatgctg attttcatgt caagagcttc gtgccacaaa cgcatactca aatcatccaa
    36061 cttaccctga agggctttca ctttgggcac ataagtaggg tctgctgaaa aagtctgagg
    36121 cgcgccagat ttgaggcgac aagacgaag
//
LOCUS       DAOPKE010000020        35401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1271_length_35401_cov_12.839529, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000020 DAOPKE010000000
VERSION     DAOPKE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_35401_cov_12.839529"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1139)
                     /locus_tag="PKY63_04320"
     CDS             complement(78..1139)
                     /locus_tag="PKY63_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HPE99873.1"
                     /translation="MIIIGITGTLGAGKGTIVDYLVRNRDFTHFSVRQFLLKEIRRRK
                     MPENRDSMVTVANELRAKHGASYIVEQLHNQAVKNGKNAIIESIRTTGEIEALRAKGG
                     FFLLAIDADARIRFQRIKQRGSETDNIDYDTFIANENREMNGADPGTQNLMKCIETSD
                     FALDNNGTFQQLFDQIERIMDEVKAREKAASAGIPYRRPSWDEYFMEVSNTVAKRATC
                     DRGRSGCVIVRDKRILVTGYVGSPIGLAHCDEVGHLFKKMIHEDGRTTTHCVRTVHAE
                     QNAITQAARYGISLDNATLYCKMTPCRTCAMLIINCGIKRVVCEFRYHDAKESEAMFA
                     EAGVHMAYFNDEILSYENQ"
     gene            complement(1373..3142)
                     /locus_tag="PKY63_04325"
     CDS             complement(1373..3142)
                     /locus_tag="PKY63_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99874.1"
                     /translation="MIRNILLLINIFLPVFSSLAQCPGCITDTTCTNNPAKPTICPAS
                     LPDGYAMQPYEADLSFYLPAQFQDSATGFNVTLNELHVTGVVGMPYGLQFTSSSANDI
                     FYPSSNPPATEYGCARFCGTPLIPGSYVITVYVTAYVTVLGMNQTSDDSFEIPLNILP
                     NPSSNAGFTISSPNGCAPHSSAFTANYESNGHPGFSYAWDFGNGNQSLLENPPAQNYT
                     SPGDYIVTLETTIDTLSYSLSSVTIVSSDCADPFDDPDYYIKIRRNSTEIFNNSGSFA
                     SNNTNPTFTFSPIILEDSTYYINVWENDDFMGGGQSGDDECGTVSFNGHTSGLQTLIS
                     GSLVITFTIQHDVLTFSNSDTITVFPTPQIQSFSAVPNDTACVSDSMQLIISGGDAWQ
                     WYLNGSAIINASNSIYQPTSSGEYHVLVSNIHGCQVQSSMMNVLVKQNPPYPTFYPSA
                     GQLVTSLTGYSYQWFLDGMPITGATGSAVDIDQTGYYSLEITATNGCSSMSSDFYAIA
                     QSIGEIEYNQVKIYPNPVQNLLFIEPAPQNAAWMIYDVTGRLIISGKLNNNSIDASQL
                     KSGVYLIRIVTNDSENSARFVKE"
     gene            3348..4895
                     /locus_tag="PKY63_04330"
     CDS             3348..4895
                     /locus_tag="PKY63_04330"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPE99875.1"
                     /translation="MTLTIENFMLIGALLLFVSIVIARNSARFGLPMLIFFLGVGMLA
                     GSEGIAGIQFNDPKAAQFIGVISLCIILFSSGLETDWSRVGPVLGRGLLLSTVGVALT
                     ATAVGLFVWQVTDFTLPEGLLLGAIVSSTDAAAVFSILREKNLALKPGLRPTLELESG
                     SNDPMAFVLVIAMLELVVNSASGFTDVIQSFIAHMAVGAVAGFGFGFVGKEIINRIKL
                     SFEGLYPILMLSLVYISFYATDLMDGNGFLAVYICGVYLGNQKLIHKRTILKMFDATA
                     WLMQIILFLTLGLLVYPSHIVPFIGIGIAISVFLILIARPIAVFISLLPFNFSSREKF
                     FISWVGLRGAVPIVFATYPMLAGIDKAGVIFNVVFFISITSVLIQGTTLPVIARWLKV
                     TVPRSMRQQTAIDRLLDDEDKSALEEIIIPEDSFAVGKRIVDIRFPATSIIAMIRRGD
                     RFVTPKGSTVIEAGDVLMILSEDRKDLPAVYSSLKIPFANAAQTPADMPLRKQAMRLR
                     SLFRKRG"
     gene            complement(4905..5894)
                     /locus_tag="PKY63_04335"
     CDS             complement(4905..5894)
                     /locus_tag="PKY63_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPE99876.1"
                     /translation="MKIKIAILLCWLLVPICGLPQDIHFSQTDNTPLTINPALAGEGN
                     CSGRFISNYRNQWRSVSANPYKTISFSGDMSSRKKKFAFGLLFFNDRTGTGHFNQTTV
                     QLCMASRVQFNRQTFLKLGIQGGWSARSIDMESLTWNSQFDGSVINPDMSSKETFVQG
                     KTGAPDFAAGMLFTHNWTNKNILNIGLSSWHVNKPQESFYANSESLPLRWCGHADMTI
                     KLPGKTLLVYPSAVFMTQASYRQFIFGSFIRYIPGLDSKYTGREKASSIYFGMHYRFG
                     DAIILSTKINYRRQLNLSFSYDINVSRFHVASRTRGAMEISLSYLIPDNRLKL"
     gene            complement(5897..8275)
                     /locus_tag="PKY63_04340"
     CDS             complement(5897..8275)
                     /locus_tag="PKY63_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPE99877.1"
                     /translation="MIRWIIILLMFLVVSPFVHGQISFAPYQIITTGSWAEVVAIDDV
                     NDDGLNDVVLGTTFNGDAANDYRIFIYLQDTSGNLLPPLKLHYGTININSIVINDVNN
                     DNRNDIIIGFGKNIGIYFQHSTGGFSSIQVYQLNNANNYPVNGLSTGDLNSDGLEDIA
                     VSYSSNDFIKVMYQQYSGGFQISRYSAPYDQNNEVEIADVNNDNKKDLIYLGCYFNRG
                     TYIFRQTVLGLDSTYEAHIDTPFTFGYNVGLAIGDLNNDGLNDIVKTNDANSPNSKFI
                     INFHHSSISDSLLPPMDFITYDSPEPVRIADLNCDGKNELITTHGGFNYISVTEQNPI
                     TYFNTYSLFFIDAATHYNTKGISIGDINGDQKKDIVLANGDLGLQILYNTSSLSSVCC
                     AQPSFSPCPIGNTIICQNDTVKYFSDSLTSNNTTWNLLPSQAGNFIFTCNDSCVIAWN
                     QSWTGTAALFTKTTNDCGIAISDTLFIEINHRPSLSLGPDEQLCSGDSLFISVNSHYN
                     TSYSWQDNSTDSLFIATMGGTYSLLASNACGTDTDTIIITEIPNPSISLPTDTFICDQ
                     NPIIIDASVPSNASYIWSNGSSNSFTEIALPGEYSVSVTDSNGCKSCDTVSITELNSP
                     YSDIIDTYTICAGTELTLNAFNYGSTYVWQDGSTDAQFTAQDSGLYYVTVSNFCGTVS
                     DSTIIKVSDCTDYLELPSAFSPNNDGINDLLIPTGRNITNIRLMIYDRWGEKVFECNC
                     GLPQNPVISECAWNGTFRGSDLEPGVYMYYLSGNSVLDGRIIQHSGNITLVR"
     gene            complement(8333..9184)
                     /locus_tag="PKY63_04345"
     CDS             complement(8333..9184)
                     /locus_tag="PKY63_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPE99878.1"
                     /translation="MIRYLRLIKFSHTIFSLPFALIGFLFATESGYELPTWLIALWIL
                     LALVAARTAAMSFNRWADHRMDAKNPRTQNREIPAGIIKPTQALMLTIVSSAVFVLLA
                     ALINDLCLWLSPVALLIILGYSYTKRFTALAHLVLGMGLGIAPAATFIAVTGTFDPGL
                     LFLCFGVMLWTAGFDIIYALQDMEFDRQEKLHSIPATLGYKRSLLLAAVFHAVSIMLM
                     FHFGFIVYGDIIALAGIVLFAALIIYQHLIVRLYGPKKINLAFGTMNGVASLVFGAAY
                     LLEFVLR"
     gene            complement(9181..10821)
                     /gene="lnt"
                     /locus_tag="PKY63_04350"
     CDS             complement(9181..10821)
                     /gene="lnt"
                     /locus_tag="PKY63_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPE99879.1"
                     /translation="MQDLKKKHLILLSFLSALLLSLAWPLRGFPFLIFIALIPLLMVI
                     EQIRQNPDRFRLMATFRYSYITFVLWNAATTYWIWNSTEVGALFAILVNSLLMALVVQ
                     FSAFIRLQLKNQIVGIAILPVLWIAFEFLHQDWDLSWTWLTLGNVFASRVSWVQWYEI
                     TGVHGGSLWIWVINILLFLLIAPCLFKKKICARGRNTLIVSVLLIFVLPSAWSFWRYY
                     TYEEKSNPVDIVLLQPNLNPYTEEYTLKVPEVMDRLFTQQSKSMVDSSVDFIVAPESV
                     LQEGMFEDEFHYSLSIMKIKEFLVHYSPESHFISGAGTFRMLKPNEPLTAAARRYNSQ
                     MYYEAYNTALFMDSLGVPVTYHKSKLVAGVEQMPFVHLIKPIEKLAIDLGGTIGTLGK
                     SKDRTVYTKGVVPVSAVICYESVYGEFVTEFVSNGAQIIFILTNDGWWKNTGGHRQHF
                     VYSRLRAVETRRSIARAANTGISCFINQRGDVIQRTEYWTRDVIRNTVNANDVITFYA
                     KNGDYIGRGMTIVSGLLLLISLLLYTANRISRRKTHLR"
     gene            10914..12632
                     /gene="lysS"
                     /locus_tag="PKY63_04355"
     CDS             10914..12632
                     /gene="lysS"
                     /locus_tag="PKY63_04355"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPE99880.1"
                     /translation="MPELSEQEILRREALTQLHELGIDPYPAALYPVTAYSGEILNEF
                     ESNPDKFKEICIAGRIMTRRIMGAASFAEIQDVQGRIQVYFKRDDLCPGEDKTLYNTV
                     FKKLLDIGDIIGVKGFAFITQTGEISVHAQSFTLLSKSVKPLPIVKEKDDKIYDAFTD
                     PEQRYRNRSLDLIVNPQVRDTFVKRSMLTNSMREFLTAKGYLEVETPILQPIYGGAAA
                     RPFTTFHNTHQQTLYLRIADELYLKRLIVGGFDGVFEFAKDFRNEGMDRFHNPEFTQM
                     ELYVAYKDYNWMAELVEEMVEKIAIDLTGSTIVKVGENEINFKRPWKRLTMFEAIHEY
                     TGIDISEMDEAALRETACKLNIQTDPSMGKGKLIDEIFGETCEGKLIQPTFIMDYPVE
                     MSPLAKKHRSKPGLVERFEAIVNGKEICNAYSELNDPIDQRKRFEEQLELGKRGDEEA
                     MMLDEDFLSAIELGMPPTAGLGIGIDRLTMIMTNSPSIQDVLFFPQMRPEKKKEVFTP
                     EQLIEAGVHADWAPVLIKMNINSVEDLKNANPNKLFNDLGGMRKKLKLEIAMPTLDDV
                     KRWCGQ"
     gene            12722..13255
                     /locus_tag="PKY63_04360"
     CDS             12722..13255
                     /locus_tag="PKY63_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99881.1"
                     /translation="MSIGKQAVLVIMVLLLAVCASAQDKVREGEVRSDTVVSLLQVID
                     DPVSVVLFRNDSYVISASFTQFRTNLSEWISNNPGIPGDEKLLELVDKAAISGKVIDA
                     AQIAEKSSLVLRLKYRMADLLQQGQCMIYDKKKYGPVANVTVQKYVYDCGPLCGESGR
                     RFLIGTTLIFEVIDWIR"
     gene            complement(13323..13694)
                     /locus_tag="PKY63_04365"
     CDS             complement(13323..13694)
                     /locus_tag="PKY63_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99882.1"
                     /translation="MEGAIITTFIIMLLVSAAIGGLILWGLARGLAKIETAKYLNSAL
                     ICLISSAVVYLVLLVFDKQLAELGLTMLSLFALVLSAIVYITVGKMIWKCSWGKSFLA
                     NIVWILINTALIYWSTSQSMQ"
     gene            complement(13921..14541)
                     /locus_tag="PKY63_04370"
     CDS             complement(13921..14541)
                     /locus_tag="PKY63_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPE99883.1"
                     /translation="MSNLNGIKNIIFDLGGVILDVDFTITQREFVKLGLKNIDQTFGQ
                     YHQIGFFDLFDRGEIDEKEFLNKASELFPASVTQQQIIDAWNAMLFDLPRHRFELLKE
                     LGKKYRLFLMSNTNIIHYKEYQEIIRKTYGISGLDALFEKAYYSFLVGMRKPEDRFFN
                     LILNENGLHANETLFIDDTSINTDAAEKLGIRSWYLKPGTDIISLQ"
     gene            complement(14519..16048)
                     /gene="ispG"
                     /locus_tag="PKY63_04375"
     CDS             complement(14519..16048)
                     /gene="ispG"
                     /locus_tag="PKY63_04375"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPE99884.1"
                     /translation="MKSSMENSWFCFKSREVLVGETSFGGGNPVRIQSMTNTPTCDVK
                     ATVEQCKRIFDAGAELVRMTARNIAEAKNLSDIRLQLSSSGYKKPLAADIHFNPEIAV
                     EAARRVEKIRINPGNFVDLFPKKLQYSESEYQQELNEIKETVLPLIQVCRQYGTAVRI
                     GINGGSLNRRVIDRYGNTTQGMVESALEFIQLFHDEHFHNLVISIKASDVKTMIHANR
                     LLAQRSIELGFDYPIHLGVTEAGEGEDGRMKSAAGIGSLLMDGIGDTIRVSLTEIPEN
                     EIPVAKEIIETASQNQTDKKTTEKFFALANPFEHYLNNTETNSQIKPIVNTATVFQSS
                     NININFLRKQYLTHPNEELLLQWNPGDCTISTFSMVAGSILADGCGNAININASTGTS
                     KHYDHAIDLLQVTNRRISKASFTSCPSCGRTTYDIENVLRQVKETFAHLKGVHFAVMG
                     CIVNGPGEMAGAKYGILGSKPNHVDIWVNGKPFLKNIHQNDVLKELKKIVAENEQSER
                     N"
     gene            complement(16084..16557)
                     /locus_tag="PKY63_04380"
     CDS             complement(16084..16557)
                     /locus_tag="PKY63_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99885.1"
                     /translation="MKRIALIPVIFSFVFLFMGASVQAQPVMKEKAKKVVNRSAIVIY
                     AAHKSTIANQVFTGNLKKAVVHQNIAIMLFLEGKYMRAIHQSRRAREFARLQLEANSG
                     RYPAGFEPESDEIPEGPQPDNSELDAEAEASEFNSIINDDEALSKEKLDNLEIKE"
     gene            16720..18261
                     /locus_tag="PKY63_04385"
     CDS             16720..18261
                     /locus_tag="PKY63_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99886.1"
                     /translation="MPLLFFLWCCSVVQAQFILSEEARFQLNPPAFSDSCLPALSIPG
                     LFPVIDLEPSRLDGDDRGSWFREKVFYDHFVKINDGSRYEFFLDPVFDFRAGSEDKNQ
                     LYKNTRGYILNGRIGENFFVRSEFYETQTKLSDYAAAWVDTMKFIPGMIRMKPFGING
                     FDYGVVFSQIMWQPFKNYGVRLGYDRFHFGKGYRSMILSDASQAYPFLMNTLTHGKWS
                     LNHNIASLHNPDFNNRLNVPLSESGVYQQKWFSFTYLTYSPKSWLNLGLFESVVFMPA
                     DSTGISFYPMSMFPLPLARTIAVESKGLHHAMIGFQADATWEEQLIFYTQIVADKIDF
                     SKLKYQQGIQGAIQLGVRLIVPEGGYVFSEFNLATNNVYTSSHAWTSYTSNDQPLAHP
                     TGQQFKEIICGFHWRENPFLLDISFNNIWAGALPVTDNYLRADSYTDVLLTGFYEPIN
                     RPDRVMHLGFTVAYFINHVTNFAVFAGMHYRNYHSTLSVTPDNSLTYEIGLRHMIRKQ
                     YYDFF"
     gene            18277..19806
                     /locus_tag="PKY63_04390"
     CDS             18277..19806
                     /locus_tag="PKY63_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99887.1"
                     /translation="MSILILIVLWLPAQVPSSPSVVAMEMNVSAMHRPFKDSIGTAHS
                     SLNNFQLVNNQSSDTEKIFFIEPIASTEIMFGSDFAHMSGAGVKMTFEKNKFSAVMLP
                     QSDFADYPGFVGEKFDSMNVFPYKGKSLISSGNASGVLSVPLYMKYELSRFFSLEGGN
                     GKAFLGDGYRSMMLSDQTAEYPFARIVANLKQIKYMHQISRWRQELSDGSNETKFAAT
                     HYLSWMITKKLNFSVFESVIWRSEDSVRQRGFEWLYLNPVMFFRPAEFALGSPDNNVL
                     GGNISWNLFSKTKVYGQFVLDEFFISEIKNWLKHLLKPDDAAIQYGAWVNKQAFQVGV
                     KSMDVFGMKGFNCLSEFNYARPYIGSHRDPMLSNTHMNQPVAHPFGANFYEFVFRSEY
                     KKTTWSAHLNMMYYLTGLDSAGTHCGQNLLQSTFDSPLNNGNIPVQYYGNAVGQGVRH
                     DVVFINAEFRKNLFWNSNILIWRIGAMYRFDKSALINASDKYIYTGIFFNMLPDEWNM
                     R"
     gene            complement(19809..21314)
                     /locus_tag="PKY63_04395"
     CDS             complement(19809..21314)
                     /locus_tag="PKY63_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99888.1"
                     /translation="MRIVRILNILVIVLFFALWIILLIRKEAYQGDGMIHPTGANPDS
                     AYFSSKIPYGIHYQGAFIGFISQNKDNKILASGLWKTDHEKQSVVHFPFPISHQFDSL
                     IAVLSAPDSSLTIVGLASDSIVKIIHFDSLVAPGLIFTAPADIKAVHYIKNQPEIVFG
                     RQKKLVGSIASIDDTVVDFRKYELPGFFDRICEIVGAWPENDKWRFLLSTDFHQKDEK
                     WILLGSKNKTNFLIFDERAVFPDFPGILNKSHHKVQKQFDYSISGLVPQKPYPDTLLM
                     IGKNNFSEKKISKPKPTNCIWFNIIGNQKTILILESDETSMQAGSISRRLFKADVSDT
                     LPFITESGKLVIHQPGEPNRQQIFMSENEFPIAWFRLSKDSNIVLTNKLNFSYLSDDG
                     RLLDRKNFFKLVNSTILRKEPGTIIFLDGHFPTLRALTWFVLLYGLIPIWIISLVIIW
                     LIEAIRPKPKFAVRERKWPFMMRLMPGSVIYLIIWLSSIYTFLQSFAVFNF"
     gene            21568..22269
                     /locus_tag="PKY63_04400"
     CDS             21568..22269
                     /locus_tag="PKY63_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPE99889.1"
                     /translation="MKKYKYVIFDLDRTLWDFDRVSHEVLSELFLELVQPHTQYSFEY
                     FHQKYAVINSGLWEQYRRHEIKKEVLRVKRFSLALEEIGLDRPWIANELADEYVKRTA
                     DHAYLFPGTLELLSYLKEKEYILSVMTNGFKEAQYPKIARSGLGPYFEYLFISEEIGF
                     NKPDIRIFEFALKKMDAMPNEVIFVGDDYEVDIEGAAAAGMDQVFFNPHTGPSDNGKP
                     ATYRISELAELMKIL"
     gene            complement(22336..23448)
                     /locus_tag="PKY63_04405"
     CDS             complement(22336..23448)
                     /locus_tag="PKY63_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99890.1"
                     /translation="MKTRLLAIVFSVLSFSLIAQTTKTIQVGGVSRQYKEFVAGSYTG
                     SNPVPLVICLHGLGDNMTNFSNIGIHLLSDSAQFITLYPQAMSSPYGTAWNSGASYMG
                     ITLNPTVDDIGFISALIDSTAAIYNIDLTRVYATGFSMGGFMSNRLACQLGTRICAIA
                     SVAGTIGASCTCNPFRATPVAHFHGTADGTVAYTGNAYGNDAEELVNFWANFNNCDTP
                     AVHTALPNTAADSMSVDLYKYLNGDLNSEVWFYKINDADHTWLIPGVNDISYSIEIWN
                     FFKKFQHTTLDIENTDAPKLSVYPNPTTDILQVNGFRGADHGMLIIMDLSGRTLMQSC
                     FENGFTTLNVSQLPAGIYLLQLSGDNVFETVRFLKQ"
     gene            complement(24285..25448)
                     /gene="dinB"
                     /locus_tag="PKY63_04410"
     CDS             complement(24285..25448)
                     /gene="dinB"
                     /locus_tag="PKY63_04410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPE99891.1"
                     /translation="MDRSVLHIDLDTFFVSVERKENSALIGKPVMVGGGSDRAVVSGC
                     SYEARAFGVHSGMPMKMARMLCNDAIVIRGDMERYSYYSRMVTDIIREGAPLYEKSSI
                     DEFYLDLTGMDRFFGTLKWSHELRERIINETGLPISFGLSVNKTVSKIATGEAKPNGE
                     LHITQPAIKPFLDPLPIQKIPMLGKKTAHLLRSMGIVNIGVLSNMPPVLLTRVLGSNG
                     LMLWKKANGQDDTPVVPYEDAKSMSTETTFETDVTDRAEMERILSSMTERLAFRMRKN
                     NRLTGTVTVKIRYADFETHTMQKRIPYTAFDHVLVQNVKDIFGKLFSRRLRVRLIGIR
                     FSELIGGNQQLDLFDETPELTRLYQSLDKIRRRFGEDAIHHAIGMHKSRSSGA"
     gene            complement(25628..27154)
                     /gene="rny"
                     /locus_tag="PKY63_04415"
     CDS             complement(25628..27154)
                     /gene="rny"
                     /locus_tag="PKY63_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPE99892.1"
                     /translation="MLVLEVVVLVIAILLWAFIFRKSAERKKKNLLEDANKEADMIKQ
                     TRILEAKEKFIQMKSEIEKRNQEQNKRILSIENKLKQREMTLNQRNEEIQRRHKESQT
                     LKDNLTAQTEIINKKLEDVSKLHKESVEKLEAVSGMSADEARTQLVNSLQEEAKIEAM
                     SHVKDIVEEAKINAHREARKIIIETIQRTATDHAGENAVSSFPIANDEIKGRIIGREG
                     RNIKALEAATGVEIVIDDTPETIILSAYDPVRREIARQALEKLVSDGRIHPARIEEVV
                     AKVKANIEQEIIETGKKTLIDLGIHNMNHELMRLVGKMKYRSSYGQNLLQHSKEVANL
                     CATMAAELGLNPKMAKRAGLLHDIGKVPDNEPDLPHAQLGMKLAEKYKEHPEIVNAIG
                     AHHEDIEMTSLIAPIVQACDAISGARPGARREVVESYINRLNDLEAIAMSYEGVQKTY
                     AIQAGRELRVIVGAEKISDGAATQLSLDLSKRIQESMTYPGQIKITVIRETRAVSYAK
                     "
     gene            complement(27407..27697)
                     /locus_tag="PKY63_04420"
     CDS             complement(27407..27697)
                     /locus_tag="PKY63_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPE99893.1"
                     /translation="MSGLVKITVTLANRKYTLSVEKEDEMVIRNATESINSRIKDFNM
                     HYPGKDMQDILAMILLNTSAALAKKDNSGDSGSKEELLSRLSEINQLLVKAV"
     gene            complement(27699..27986)
                     /locus_tag="PKY63_04425"
     CDS             complement(27699..27986)
                     /locus_tag="PKY63_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99894.1"
                     /translation="MKETNQLLSEIDNNVRKLIAQLNLYRKEIEFLKEEGRRVQAENE
                     NLKQQLTNGEILLTARQIAEGVENRNDSESTRKKLNELIREIDKCILMLED"
     gene            complement(28106..29185)
                     /locus_tag="PKY63_04430"
     CDS             complement(28106..29185)
                     /locus_tag="PKY63_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99895.1"
                     /translation="MKKLLLSFVLVAISATLFAQLLTPKQMDSKLLESRKYVETQSVK
                     APFDTMGWTLGYLPCFGDPTPAFHIYGLVDASDNDLGYWFGVNYDPSDTSSIDYWAMG
                     YFVDQPVSITGVIIGIAGKTILTGGASSKIELSIYDIADDNTVIAASTYAPGPDMYSG
                     TALATVELAASAIDTAWANLGFNYVPLSAPLWVNQDFSIVSNFEGMRLATDTAYMYCD
                     EPGNHNGMNYCFYNQDPFAYYWVSLSYGSSGALDVNLPLFAVVDMGSAGINDNFFQGM
                     KLTTSPNPASDMLVMEYALQFDSNVKVQIIGSNGAVVKEVELGEQNASNYYKESIDIS
                     DLASGNYFVSLMSNGNRLTKQIVVE"
     gene            complement(29302..32163)
                     /gene="gcvP"
                     /locus_tag="PKY63_04435"
     CDS             complement(29302..32163)
                     /gene="gcvP"
                     /locus_tag="PKY63_04435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPE99896.1"
                     /translation="MITNNFTFRHIGPSEEEKQTLLHAIGVKSAEELIEKTIPKSIIN
                     PKKLNIGPGLNEYETQSLLKNIGSQNKIFKSFIGMGYYGTIVPGVILRNILESPGWYT
                     SYTPYQAEISQGRLEALLNFQTVVSDLTGMPIANASLLDEATAAAEAMIMAYNTRKRD
                     KVKAGANQFVVSHGVLPQSLEVIRTRAIPLGIEVVVDCVKDFVFNDKTFGALVQYPNQ
                     YGKIHNYSDFADKVHANGSMLAVAADLMALTLLTPPAEWGADIVIGSTQRFGIPMGYG
                     GPSAAFMATKEEFVRTMPGRIIGISKDALGNKALRMTLQTREQHIKRERATSNICTAQ
                     ALLAIMAGMYAVYHGPDGLKKIARHINILAGVLSNEIQKTGYKQINETFFDTLQIEIP
                     DGVNMDDIRTRALQNEMNFRYIDNKLIGISIDETTTLDDINTIITILASAAGKQHTPF
                     VCNPAECEKIRTFDASFDRKSDFLKHEVFHSHRSETDMMRYITNLRNKDLALDRAMIP
                     LGSCTMKLNAATELFYLSWPEFGNIHPFVPADQAKGYLTLLDELEQLLAEITGFDKVS
                     FQPNSGAAGEYTGLMVIRAYHESRGDAARNVCLIPSSAHGTNPASAAMAGMKIVVVKA
                     DEKGNVDIADLKLKAEEHAKDLAALMITYPSTHGIFEESIKEIMDIIHSHGGQVYMDG
                     ANMNAQVGYTSPGFIGADVCHLNLHKTFAIPHGGGGPGVGPIGVAKHLSPFLPGHVIV
                     KTGGEKAISAVAASPFGSAYVIPISYAYIKLMGAEGLTMATEYAILNANYLMKKLEPD
                     FNILYKGEHGFCAHEFIVDCNPFHKAGITETDIAKRLMDYGFHAPTLGFPVHGTLMIE
                     PTESEPLSEMNRFIDAMLAIREEIRDVAEGKCDKENNVLKNAPHTVEMVATDNWNKPY
                     SREKATCHRKDALKYWPTVTRIDDAYGDRNIFCTCAPVE"
     gene            32264..32884
                     /locus_tag="PKY63_04440"
     CDS             32264..32884
                     /locus_tag="PKY63_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99897.1"
                     /translation="MLRFCIVFFVIILVVCGLAQAQDTLYLMSGRLKTNIHVLEMDSS
                     KIAYAPGRTLKVNRRGLVRTMYKERQDVFEIRYEDGTRELAYIMDSSGYIITPEQAGS
                     YVDGCHDAFLYSHNRIVGPACYLITLGSIFVLPPIAVIAVPCVYSAATIMFTPEFPAD
                     RVDEERVDKYYILGYQDTRKIKKVKSSMFFGIAAIVTGFVITFTTK"
     gene            32943..33893
                     /gene="ftsY"
                     /locus_tag="PKY63_04445"
     CDS             32943..33893
                     /gene="ftsY"
                     /locus_tag="PKY63_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006846481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPE99898.1"
                     /translation="MALFGIFGKEKKESLDDGLKKTRESVFARITRAVAGKSKVDDDV
                     LDNLEEALISSDVGVETTLKIIERIQQRVSRDKFMSTDELNHILRDEIVLMLDENRSQ
                     RAVQPGDVPTYVILVVGVNGVGKTTTIGKLAYRFKNAGKSVVIGAADTFRAAAVDQLK
                     VWAERAGVDIVEKGMNADPAAVAYDTAAFAKAKGYQVAIIDTAGRLHNKINLMNELGK
                     IKKVIQKVFPDGPQEVLLVLDASTGQNAFEQAKQFTAVTDVNALALTKLDGTAKGGVV
                     IGISDRFNIPVKYIGIGEKIDDIQIFDSAEFVDSLFKSGQ"
     gene            34017..35309
                     /gene="rimO"
                     /locus_tag="PKY63_04450"
     CDS             34017..35309
                     /gene="rimO"
                     /locus_tag="PKY63_04450"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPE99899.1"
                     /translation="MKKNKVGIITMGCPKNLVDSEKLAFALQKAGFEVEHEQTDADII
                     IVNTCGFINDAKEESINEILNHTARKNAENNLKVIVTGCLSERYRDELKNEIPEADAI
                     FGVNEYDRVIEFVTGKSENPQQERILGTPSHYAYLKISEGCNRTCSFCAIPMIRGPHV
                     SLPEEDILKEAQWLALHGVKELIVIAQDTVSYGLDLYGERRIARLLHKLSGIVGIEWI
                     RLMYTYPAGFPDDLIDEIAQNPKIVKYVDLPLQHISTSIMGSMKRSIDRESTEALISK
                     IRNRIPGVAIRSTFIVGYPGEKASDYAELVQFIKDSRFERFGVFAYSPEENTAAFELK
                     DNVPHKTKMQRMSGLLRYHEKASIDFNSKLVGKTLSVIVDEFNKEEKCYYGRTAIDAP
                     EIDNLVRLFVDGDKVLRAGDIVDAKVISSGIFDIDCEC"
BASE COUNT         9500 a         7098 c         8131 g        10672 t
ORIGIN      
        1 aaccttgttc ttgacctcga tctcgctgaa agccactcac cgctcgctat tcactgctca
       61 ctgctgtcga ttctcgctta ttgattttcg tagctcagaa tttcatcatt aaaatacgcc
      121 atatgcacgc cggcttcagc aaacatggcc tcgctttctt tggcatcgtg atagcggaac
      181 tcgcacacca cgcgtttgat gccgcaattg atgatgagca tggcgcaggt gcggcagggt
      241 gtcattttgc aatacagggt tgcgttgtcg agcgagatgc cataacgcgc tgcctgtgtg
      301 attgcgttct gttcggcatg aaccgtacgc acacaatgtg tggtggtgcg gccatcctcg
      361 tgtatcattt ttttaaacag gtgcccgact tcgtcgcagt gcgccagtcc gatgggtgag
      421 cccacataac cggtgaccag aatgcgtttg tcgcgcacaa tcacacagcc cgatcggccg
      481 cggtcgcagg tggcgcgctt ggcaacggtg ttggagactt ccataaaata ttcatcccac
      541 gatggacgac gatagggaat acctgccgaa gccgctttct ccctggcttt cacctcgtcc
      601 ataatgcgtt cgatctgatc gaagagctgc tgaaaagtac cattgttgtc gagcgcaaaa
      661 tcggatgttt caatgcattt catcaggttt tgtgtgcccg gatcggcgcc gttcatttcc
      721 cggttttcgt tggcaataaa ggtgtcgtag tcaatgttgt cggtttccga accgcgctgt
      781 ttgattcgtt gaaaacggat gcgtgcatcg gcatcaatgg ccagcagaaa aaacccgcct
      841 ttagcgcgca gggcctcaat ctcgcctgtg gtgcggatgc tctcgatgat ggcattcttt
      901 ccgtttttta cggcctgatt gtgcagttgt tcaacaatat acgatgctcc gtgttttgcg
      961 cgtagttcgt tggctacagt taccatgctg tcgcggtttt cgggcatttt acgccggcgt
     1021 atttctttca gcagaaactg ccgaaccgaa aagtgtgtaa aatcgcggtt gcgaaccaga
     1081 taatcaacaa tggttccttt gcctgcaccc aatgttcctg tgattccgat gatgatcata
     1141 tcaaataatt cttgaatgca aatgtagtga aaagtggatg aatccgattt ttacaagaga
     1201 tattcaaatg aatttgcttg tgaaacaagc cacgttgtgt tacatacctc atgtcttacg
     1261 catgttacct tatgcctgga gtttatcccg acgaaggcgg gaccttacgc cttacgcctc
     1321 atgccttacg ccttatgcct catgccttac gcctcatgcc tagcgccttt tatcattctt
     1381 ttacaaagcg tgcagaattt tccgaatcat tagtaacaat gcggatcaga tacacaccgc
     1441 ttttaagctg cgatgcgtcg atggaattgt tatttagttt gccggaaata attagacggc
     1501 cggttacatc atagatcatc catgcagcat tttgtggtgc aggttcaatg aaaagcaggt
     1561 tttgtactgg atttggatat atttttacct gattgtattc aatttcgcca atggactgag
     1621 caatggcata aaaatctgag gacatgcttg agcagccatt tgtagctgta atttcaagcg
     1681 aataatatcc ggtctgatct atatcgacag ctgatcctgt ggctcctgta atgggcatcc
     1741 catccaaaaa ccactgatat gaataacccg taagcgaagt tacaagctgg ccagcagaag
     1801 gatagaatgt tggatatggt ggattctgtt tcacaagcac attcatcatg gaggactgaa
     1861 cctgacatcc atggatattt gatactaaca catgatattc tcctgatgaa gtgggttgat
     1921 aaatggaatt actggcgttg atgatggccg atccattcag ataccattgc catgcgtcgc
     1981 ccccggaaat gatgagctgc attgaatctg acacacaggc agtatcgttt ggaacagctg
     2041 agaatgattg gatttgcggt gttggaaata cagtaattgt atcactgttt gaaaaggtta
     2101 atacgtcatg ctgtattgta aatgtaatga ccagcgaacc gcttattaaa gtttgtaagc
     2161 cagatgtatg accattgaaa gaaacagtgc cgcattcatc atcgcctgac tgtcctccac
     2221 ccataaagtc atcattttcc cacacgttga tatagtaggt ggaatcttca agaatgatgg
     2281 ggctgaatgt gaaagtagga tttgtattat ttgaagcgaa tgatccgctg ttgttgaaaa
     2341 tttccgttga atttcttctg attttgatat aataatccgg gtcatcaaaa gggtcggcac
     2401 aatcggagct tactattgta acagaggata atgagtagga aagagtgtca atagttgttt
     2461 caagtgttac tatataatcg ccggggcttg tgtaattttg tgccggtgga ttttcaagca
     2521 aactttgatt gccgtttcca aagtcccatg catatgaaaa ccctggatgg ccattgcttt
     2581 cataattggc tgtgaatgca gaggagtgtg gtgcgcatcc gtttgggctg ctgatggtaa
     2641 agcccgcatt gcttgatgga tttgggagaa tattgagtgg tatttcgaat gaatcatccg
     2701 atgtttgatt catgccgagg acagttacat aagcagttac atacacggtg attacgtatg
     2761 agcccggaat gagcggagtg ccgcaaaaac gcgcgcatcc atattctgtg gcaggcgggt
     2821 tggatgatgg gtaaaaaatg tcgttggccg aactggatgt aaactgcaat ccatagggca
     2881 ttccaacaac tccggtgaca tgaagctcgt tcagggtaac attaaaacca gtggcagaat
     2941 cctgaaactg tgcgggcaga taaaaggata ggtctgcctc gtatggctgc atggcatagc
     3001 cgtcggggag cgaggctgga cagatggttg gtttggccgg attgtttgtg caggtagtat
     3061 ccgtgatgca gcccggacat tgggccagtg atgaaaaaac cggaagaaag atattgatta
     3121 gcaacagtat atttcgtatc atagaaaatg atattggttg cgcaaaatta aacagtattt
     3181 cgcaatgacc agtcaattat ttgttattgt acttagcgtc tggtattcaa tattcaccct
     3241 ttacatcaat aaaatcaagg tttttcagaa gtgtattttt gtaatgccgg attctgtttg
     3301 tgctaacagg aaaatttgat taaatttgtc ctgctcaatt tctgacaatg actttgacta
     3361 tcgagaactt tatgctgatt ggggcattgc tgctttttgt gagcattgtc attgcacgca
     3421 attcggcacg gtttggattg ccgatgctga ttttttttct gggagtgggc atgcttgccg
     3481 gtagtgaagg tattgccggt attcagttta acgatccaaa ggcagctcag tttattggag
     3541 ttatcagttt gtgcattatt ttgttttcat cgggactcga aactgattgg agcagagttg
     3601 gtcctgttct tggacgtgga ttgttgttat cgactgttgg tgttgcgctc accgcaacgg
     3661 ctgttggtct ctttgtctgg caggtgactg attttacgtt accagagggg ttgttgctcg
     3721 gggctattgt gtcttcaacc gacgctgctg ccgttttttc aattctccgg gaaaaaaatc
     3781 ttgccttaaa acctggcttg cgtcctacat tggagcttga aagtggcagc aatgatccta
     3841 tggcttttgt tctggtgatt gccatgctcg aattggttgt gaattcagca tcgggcttca
     3901 ccgatgtgat ccaatccttt attgcacata tggctgttgg ggctgttgca ggctttggct
     3961 ttggctttgt gggtaaagaa attatcaatc ggataaaatt atcattcgaa ggattgtatc
     4021 ctatcctgat gctctcgctg gtgtatattt cgttttacgc caccgacctg atggatggaa
     4081 atgggtttct tgctgtttat atttgtggcg tgtatcttgg caatcagaaa ttgatacata
     4141 agcggacaat tctcaaaatg tttgatgcaa cggcatggct tatgcagatt attcttttcc
     4201 ttactcttgg tctgcttgtt tatccgtctc atattgttcc gtttattggt attggtattg
     4261 ccatttctgt ttttctgatt ttgattgcgc gaccaattgc agttttcata agtttactcc
     4321 cctttaattt ttcctcgcgc gaaaagtttt ttatttcatg ggtcgggttg cggggtgctg
     4381 tccctattgt gtttgctact tatccaatgc ttgcgggaat agataaagcg ggagtcattt
     4441 ttaatgttgt tttttttatt tccattactt ctgtgttgat tcagggtaca acattgccgg
     4501 ttattgcccg atggctgaag gttacagttc cgcgctccat gcgacagcaa acagctattg
     4561 accggctttt ggatgatgaa gataaatcag cattggaaga aattattatt cctgaagaca
     4621 gctttgctgt aggaaagcgt attgtggata tccgttttcc agccacttct attattgcca
     4681 tgataaggcg cggtgaccgg tttgtgaccc ccaagggatc aacggtcatt gaagctggtg
     4741 atgtgttgat gattttatcc gaagatcgta aagatcttcc tgctgtttac agtagtttga
     4801 aaatcccatt tgctaacgca gcacaaactc cagcagatat gccgctccga aaacaagcga
     4861 tgcgactccg ttcattgttc cgaaagcgag gttgacagaa gctgttataa tttaagccgg
     4921 ttatcaggaa tcaggtaaga taaagaaatt tccatggccc ctcttgtacg cgaggcaaca
     4981 tgaaaacggg aaacattgat gtcgtatgaa aatgacaaat tcaactgacg ccggtagttt
     5041 attttcgtac tgagaattat tgcatctcca aatctgtaat gcataccgaa gtaaatactt
     5101 gaagcttttt ctcggccggt gtattttgaa tcaaggccag gaatatatct tatgaaagag
     5161 ccgaatataa attgtctgta cgaagcctgt gtcatgaaaa cagcagatgg atataccaga
     5221 agtgttttgc ctggtagttt tattgtcata tcagcatgac cgcaccatcg caagggtaat
     5281 gattctgaat ttgcgtaaaa actttcttgc ggtttattta catgccatga cgacaatcca
     5341 atattgagta tgtttttatt cgtccagtta tgtgtaaaca gcattccggc tgcaaaatca
     5401 ggagctccgg tttttccctg tacaaatgtt tctttcgaag acatgtccgg atttattacg
     5461 gatccgtcaa actggctgtt ccatgtgagc gattccatat cgatgctgcg tgctgaccat
     5521 ccaccctgaa ttccaagttt taaaaatgtc tgtcggttga attgtacccg ggaagccata
     5581 caaagctgaa ctgtggtttg attgaaatgt ccggtacctg tgcggtcatt gaaaaacaac
     5641 aatccgaatg caaacttctt tttccggctg gacatgtcac ctgaaaatga aatggtctta
     5701 taaggatttg cagatacact tctccattgg tttctgtaat tgctgatgaa cctcccgctg
     5761 cagttaccct ctccggccaa tgccggattg atagtcagcg gggtgttgtc tgtttgtgag
     5821 aaatgaatat cctgaggtaa tccacatatc gggacaagca accagcacag taatattgca
     5881 atttttattt tcatttctac cggacaagag taatgtttcc gctgtgttga ataattctgc
     5941 cgtcaaggac ggagttgcct gaaaggtagt acatatagac tcccggctca aggtcgcttc
     6001 ctcggaatgt gccattccat gcgcattcac tgatgaccgg gttttgcggt aaaccgcagt
     6061 tgcattcaaa aaccttttcg ccccaccggt cataaatcat gagacgaatg tttgtaatat
     6121 ttctgccggt cgggatcagc agatcattga tgccgtcatt attgggagag aatgctgatg
     6181 gaagttcaag ataatcggta caatccgata ctttaatgat cgttgaatca gagacagtac
     6241 cgcagaagtt tgaaactgta acataataaa ggccactgtc ctgagctgtg aattgcgcat
     6301 ccgtgctgcc atcctgccac acgtaggtgc tcccataatt aaaagcatta agcgttagtt
     6361 cggtgccggc acagatggta tatgtatcga taatatcaga atagggagaa ttgagttctg
     6421 ttatgctgac cgtatcacaa cttttacatc cgtttgaatc tgttaccgag actgaatatt
     6481 cgccaggaag tgcaatttct gtaaaagaat tcgatgatcc gttactccaa atataggagg
     6541 cattacttgg aacagatgca tcaattatta ttggattctg gtcacagata aaagtgtctg
     6601 ttggtaacga tattgaggga tttggaattt ctgtaattat tattgtatct gtgtcagttc
     6661 cacaagcgtt gctggcaagc agtgagtatg tgcctcccat agttgcgata aacaatgaat
     6721 ctgtagaatt atcctgccaa ctataagaag tgttataatg tgaatttaca gaaataaaaa
     6781 gactgtctcc actgcacaat tgttcatctg gccccagcga taacgaaggt cgatgattta
     6841 tttcaatgaa caaagtgtct gaaatagcaa ttccgcaatc atttgtagtt tttgtaaata
     6901 atgcagcagt tcctgtccat gattgattcc aagcaatcac acaagagtca ttacatgtaa
     6961 aaatgaaatt tccagcctgg gatggcaata aattccatgt tgtattattc gatgtcaagg
     7021 agtctgaaaa gtacttaaca gtatcatttt ggcaaattat agtattgcca attgggcatg
     7081 gagagaaaga tggttgagca cagcaaacac tactaagaga actagtatta tacaatattt
     7141 gcagcccgag gtcaccattg gctagtacta tgtctttctt ctgatcgcca ttaatatctc
     7201 caattgagat accttttgta ttgtaatgtg ttgcggcatc aataaaaaac aggctgtatg
     7261 tattaaaata tgttatcgga ttttgttccg tgactgaaat gtaattaaag ccgccgtgag
     7321 tagtaatcaa ttcatttttt ccgtcacaat tcaaatctgc tatcctcact ggctctggtg
     7381 aatcatatgt aataaagtcc ataggaggca ataatgaatc agaaattgac gaatgatgaa
     7441 aattaatgat gaattttgaa ttgggggaat ttgcatcatt cgttttcacg atgtcgttaa
     7501 ggccatcgtt gtttaaatct cctatggcta aaccaacatt gtagccaaac gtaaaaggag
     7561 tgtcaatgtg agcttcatat gtggaatcca gaccaagtac tgtttgtctg aaaatatatg
     7621 tccctctgtt aaaatagcat cccaaatata ttaaatcctt tttattatcg ttgttgacat
     7681 cagcaatttc aacttcattg ttttggtcat aaggggcaga gtatcttgaa atctgaaatc
     7741 ccccggaata ttgctgatac ataactttaa taaaatcatt tgatgaataa gaaactgcaa
     7801 tatcctccaa tccatcagag tttaggtccc ctgtacttaa cccattgaca ggataattat
     7861 tagcgttatt aagttgataa acctgaatac ttgaaaaacc accagttgaa tgttgaaaat
     7921 agattccgat gtttttgcca aaaccaataa taatatcatt gcgattgtca ttgttgacat
     7981 cattaattac aatagaattt atatttattg ttccataatg aagttttaat ggcggcaata
     8041 aatttccgct tgtatcctgt aaatagataa aaattctata atcattggca gcatcaccat
     8101 tgaatgttgt tcctagtacc acatcattga gaccatcatc gttgacgtca tcaatggcca
     8161 cgacttcagc ccatgaaccg gtggtgatta tttgataagg cgcaaaacta atctgcccat
     8221 gaacaaatgg tgaaaccact aaaaacatca ataaaattat aatccaacgt atcataatcg
     8281 attacaatat tgttgtcaaa attaataaat aaatgtttca aaatggaaaa ttctatcgca
     8341 gcacaaactc cagcagatat gccgctccga aaacaagcga tgcgactccg ttcattgttc
     8401 cgaaagcgag gttgatcttt ttcggtccgt agagtcggac aatcagatgc tgataaatta
     8461 ttaatgcggc aaaaagaaca atgcccgcaa gcgcaatgat atcgccgtaa acgatgaatc
     8521 cgaaatgaaa catcagcatg attgaaacag catgaaacac ggcggcaagc agcaatgagc
     8581 gcttatatcc cagtgttgca ggaatggaat gcagcttttc ctgccggtcg aattccatat
     8641 cctgcagtgc gtaaatgatg tcgaatcctg cggtccagag catcacgcca aagcacagaa
     8701 acaaaagacc gggatcgaaa gttccggtga ctgcaatgaa agtggctgcc ggcgctattc
     8761 ccagccccat gcccagaaca agatgtgcca gcgctgtgaa tctttttgtg taactgtaac
     8821 caagtataat cagcaatgct accgggctga gccacaaaca caaatcattg ataagtgctg
     8881 caagcagaac aaacacagca gatgaaacaa tggtgagcat cagtgcttgc gtgggtttta
     8941 taatgcctgc cggaatctcc cggttttgtg tgcgcggatt ttttgcatcc atccggtgat
     9001 cggcccagcg gttgaacgac atggcagctg tgcgtgcagc gaccaatgca agcaggatcc
     9061 agagcgctat gagccaggtt ggcaattcgt agccggattc cgttgcaaag agaaatccga
     9121 tcagcgcaaa aggaaggctg aaaatggtgt gcgagaattt tatcagtctt agataccgga
     9181 tcatctcaag tgcgtttttc ggcgactgat gcggtttgca gtgtaaagca gcaatgaaat
     9241 cagcagcagt aaccctgata ctatggtcat gccgcgtccg atataatctc cgtttttggc
     9301 ataaaaagta atgacatcgt ttgcattgac ggtgtttctg atcacatcgc gggtccagta
     9361 ttctgtgcgc tgaatgacat cgccgcgctg attgatgaag cacgaaatgc ccgtgttggc
     9421 cgcacgcgca atggatctcc gggtttcaac cgctctgagt cttgaataca caaaatgctg
     9481 acggtggcct ccggtatttt tccaccagcc gtcgttggtt agaataaaaa taatttgagc
     9541 gccattggaa acaaattcag ttacgaattc tccataaacg ctttcatagc aaataacagc
     9601 actgactggc accacgcctt ttgtgtaaac ggttctgtct ttgctttttc caagagtccc
     9661 gatggtgcct cccagatcga tggctagttt ctcaatcggt tttatcagat gtacgaatgg
     9721 catttgttca acaccggcca ccagcttgct tttgtgatat gtaaccggaa cgccgagcga
     9781 atccataaat aatgcggtgt tgtatgcttc gtaatacatt tggctattgt atcgtcttgc
     9841 agctgctgta agtggctcat tgggcttcag catgcgaaaa gttcctgcgc ctgaaatgaa
     9901 atgtgattct ggcgaatagt gaaccagaaa ctcctttatt ttcattatgc tgagcgagta
     9961 atggaattca tcctcaaaca taccttcctg taatacgctt tctggtgcaa caatgaaatc
    10021 gacactcgaa tctaccatgc ttttgctttg ctgcgtgaat aggcgatcca tcacttcagg
    10081 tactttcagc gtgtattctt cggtataggg attcagattg ggttgcaata aaacgatgtc
    10141 aaccggattt gatttttcct cgtaagtgta atatcgccag aaagaccacg cagaggggag
    10201 gacaaatatt aataatactg aaacaatgag cgtatttctg ccgcgcgcac agattttctt
    10261 tttaaaaaga cacggggcaa tcagaagaaa aagcaatatg tttataaccc agatccagag
    10321 cgaacctccg tgtactcctg taatttcata ccattgaacc caggacacac gcgaagcgaa
    10381 aacatttcca agtgtaagcc acgtccacga caaatcccag tcctgatgca ggaattcaaa
    10441 tgcaatccat aaaacaggca atatggcaat tccgacaatc tggtttttaa gttgcagacg
    10501 gataaatgct gagaactgta ccacgagcgc catcaggaga ctgtttacaa ggatggcgaa
    10561 taacgcaccg acttcggtgg aattccagat ccagtaagta gtggcagcgt tccataaaac
    10621 aaaagtgatg taactgtagc ggaaagtggc cattagtctg aatctgtcag gattctgccg
    10681 gatttgttca ataaccatca gcaatggaat cagggcaata aaaatcagaa acggaaatcc
    10741 ccgcagcggc catgcaaggc tcaatagtag cgctgacaaa aaactgagaa gaatcagatg
    10801 ttttttcttc agatcctgca tattcttttt tttgcgaagt tacaaaattg atgactaatc
    10861 acgttttgtc caaccaatca ttccttgtat ctttgcaaaa aaaattcaga cttatgcccg
    10921 aattatccga acaggaaatc ctgcgccgcg aagcgctgac ccaattacat gaacttggca
    10981 ttgatcctta cccagcagca ttgtatcccg ttaccgctta ctcaggtgaa atattgaatg
    11041 aatttgaatc caatcctgat aagtttaagg agatttgcat tgccgggcgt atcatgacgc
    11101 gccgtatcat gggtgctgcc tcgtttgcag agattcagga tgtgcaggga cgtattcagg
    11161 tgtatttcaa acgcgatgat ttgtgcccgg gcgaagacaa gactttgtat aataccgttt
    11221 ttaaaaaact gctcgatatt ggcgatatca ttggtgttaa gggatttgct tttataacac
    11281 agaccggtga aatttcggtt catgcgcagt cattcacatt gctaagcaaa tcggtgaaac
    11341 cacttcccat tgtcaaggaa aaagatgata agatttacga tgcattcacc gatccggaac
    11401 agcgctaccg caaccgttcg cttgatctga tcgtcaatcc gcaggtacgt gatacttttg
    11461 tgaagcgttc gatgctcacc aattccatgc gtgaatttct tacagctaaa ggatatctcg
    11521 aagttgaaac acctattctg caacccattt acggaggtgc agctgcgcgg ccttttacaa
    11581 cttttcacaa tacacaccaa cagactcttt atctccggat tgctgatgaa ttatatctga
    11641 aacgtttgat tgtaggcggc tttgacggag tttttgaatt cgcgaaggac ttccgtaacg
    11701 aagggatgga ccgattccat aatcctgaat tcacgcagat ggagctctat gtagcgtata
    11761 aggattacaa ctggatggct gaactggtgg aggaaatggt ggagaaaatt gccattgacc
    11821 tcacaggttc taccattgtg aaagtcggag aaaatgaaat caatttcaaa cgcccatgga
    11881 agcggctcac aatgttcgaa gccattcatg aatataccgg catcgatatt tctgagatgg
    11941 atgaggctgc tttgcgcgag accgcatgca agcttaatat tcagaccgat ccttcgatgg
    12001 gaaaaggtaa actgattgat gagatttttg gtgaaacctg cgaaggcaag ctgatacagc
    12061 cgactttcat catggattat ccggttgaga tgtcaccgct ggcaaagaaa caccgcagca
    12121 aacccggact ggttgagcgt ttcgaagcta tcgtgaatgg taaagaaatc tgtaatgcct
    12181 acagcgagct caacgatccg attgaccagc gcaagcgctt tgaagaacaa ctcgaattgg
    12241 gcaagcgcgg tgatgaagaa gccatgatgc ttgacgaaga ttttctgagt gccattgaac
    12301 tgggaatgcc gcctaccgcc ggacttggaa taggtatcga ccgtctgact atgatcatga
    12361 ccaacagtcc ttcgattcag gatgtattgt ttttccctca gatgagaccg gaaaaaaaga
    12421 aagaggtatt cacacccgaa caattgattg aagctggtgt tcatgccgac tgggctccgg
    12481 tgctgattaa aatgaacatc aattcagtgg aggatttgaa aaatgcaaat ccgaacaagc
    12541 tgttcaatga tctgggcgga atgcgcaaaa agctgaaact ggaaattgcc atgcccacac
    12601 tggatgatgt gaagcgctgg tgcggtcagt aacacgcagc tgtgcgaagt ataaacttcg
    12661 cacagcgcta ctaacttcgc acagcgctgc caactttgta aagcgattaa attcttttgc
    12721 tatgagcata ggtaaacaag ctgttttggt cataatggtt ttattgctgg ccgtatgcgc
    12781 ttcggcacag gataaggtgc gtgaaggcga ggtccggagc gatacggttg tatcattgct
    12841 gcaggtgatt gatgatccgg tttcggttgt tttattcaga aatgacagtt atgtgatatc
    12901 tgcttctttc acgcaattca ggacgaatct gtctgaatgg atcagtaata atccaggcat
    12961 tccgggcgat gaaaaattgc tggaacttgt tgataaggcc gccatcagtg gaaaagtgat
    13021 tgatgccgct cagatagcag agaagagcag tcttgtactc agactgaaat acagaatggc
    13081 tgatttattg caacagggcc aatgtatgat ttatgataaa aagaaatatg gtccggttgc
    13141 caatgttact gtacagaaat atgtgtatga ttgtggtcct ctgtgcggtg aaagcggccg
    13201 aagatttttg atcgggacta ctttgatttt tgaagttatt gactggatca gataaaaaaa
    13261 ttgcacggaa ttaaaaaaac cccgcacaat ttttataagc gctattcgta ttaattgggc
    13321 tattattgca ttgattggct ggttgaccaa taaatgagag ctgtattaat cagaatccaa
    13381 acaatattgg cgagaaatga ttttccccag gagcatttcc agatcatttt tccaacagta
    13441 atatacacaa ttgctgaaag aacaagtgca aatagactga gcattgtgag tccaagttct
    13501 gccaattgtt tgtcgaaaac aagtaagaca agatatacaa ctgcacttga gatgaggcag
    13561 atgagtgcgg agttgagata ctttgctgtt tcaatttttg ccagtccgcg ggctagtccc
    13621 cagagaatga gtccgccaat tgctgcgctc acaaggagca taataatgaa agtggtaatg
    13681 attgcgcctt ccatagtttt tttttttaat atcctactcg tttggctttt cggattacgc
    13741 cctgacaaat gtttctgtca gaaaagtgtt tattaagttc aaaattatat tttattagat
    13801 taattccttc agtagatatg ttatattaat gtaaaaatga atagttatca acaaaatagt
    13861 tcatttgatt cacaatttgc aatgcgatca atctggtttt gacaattttt ccggaaggtt
    13921 ttattgaagc gatatgatat cggtgcccgg cttgagatac cagcttcgga tgcccagttt
    13981 ttctgctgca tcggtattga tggatgtatc atcaatgaaa agtgtctcat ttgcatggag
    14041 tccgttttca ttcagaatga gattgaaaaa acgatcttcg ggtttacgca tgccaaccag
    14101 aaatgaataa taagcttttt cgaagagtgc gtcaagtccg ctgataccat aggttttgcg
    14161 aataatttcc tgatactctt tgtaatgaat aatatttgta ttgctcatta aaaaaagacg
    14221 gtattttttg ccaagttctt tgagcaattc aaaacgatgg cgcggcaaat caaacagcat
    14281 ggcattccat gcatcgatga tctgttgctg tgtaacagag gccggaaata attcagatgc
    14341 tttattgaga aattcttttt catcaatttc tcctcggtca aataaatcaa aaaaacctat
    14401 ctggtgatac tgcccgaatg tctgatcaat atttttaagg ccaagcttta caaattcgcg
    14461 ttgggtaatt gtaaaatcaa catcgagaat cacgccccca agatcaaaaa tgatgttttt
    14521 aattccgttc agattgctca ttttctgcta caattttttt cagttctttc agtacatcgt
    14581 tttgatgtat gtttttcaga aatggttttc cattgaccca gatgtccaca tgattgggtt
    14641 ttgacccaag aatgccgtac ttcgctccgg ccatttcacc gggaccgttt acaatgcatc
    14701 ccatgacggc aaaatgtacg ccttttaaat gcgcaaaagt ttcttttact tgtcttaaaa
    14761 cattttcgat gtcataggtt gttcttccgc atgaaggaca ggatgtaaag ctggcttttg
    14821 atattcttcg gttcgtaact tgcaataaat caattgcgtg gtcgtagtgc ttgcttgttc
    14881 cggttgaagc attgatattg attgcattgc cacagccatc tgcaagaatg gatccggcaa
    14941 ccattgaaaa tgtgcttatc gtacagtctc cggggttcca ttgaagcaat aattcttcgt
    15001 ttgggtgagt taagtattgc tttcgcaaaa aattgatgtt tatgtttgaa ctttgaaaga
    15061 cagtagctgt attgacgatg ggtttaatct gagaatttgt ttccgtatta ttcagatagt
    15121 gttcaaaggg atttgcaagt gcaaagaatt tttccgttgt ttttttgtct gtctgatttt
    15181 gacttgccgt ttcgatgatt tccttagcca caggaatctc attttcgggt atctcggtta
    15241 gcgaaaccct tatggtatca ccaattccat ccattagcag ggacccgatt ccggcagccg
    15301 atttcattcg gccgtcttcg ccttcacctg cttcggttac tccgagatga atggggtagt
    15361 caaagccgag ttcaatggat cgttgcgcca gaagccggtt tgcgtgaatc atggttttca
    15421 catcgctggc tttaattgaa attaccaggt tgtgaaaatg ttcatcatga aacaactgaa
    15481 tgaattccag tgccgattca accatacctt gtgttgtatt tccatagcga tctatgactc
    15541 tgcggttgag cgatccaccg ttgattccta ttcgcacagc cgttccgtat tggcggcaaa
    15601 cctgaatcag cggtaaaact gtttctttta tttcattcag ttcctgttga tattcagatt
    15661 cggaatactg tagttttttg gggaataaat cgacaaaatt gcctggattg atgcggattt
    15721 tttcaactcg gcgtgcggct tcaacagcta tttcggggtt gaaatgaatg tcggcagcca
    15781 atggtttttt atacccggaa gaagacagtt gcaagcgaat atcggataaa ttttttgctt
    15841 cggcaatatt gcgggcagtc attctaacaa gttctgcgcc ggcatcaaaa atgcgtttgc
    15901 attgttcaac tgtcgcttta acatcgcagg tgggtgtatt tgtcattgat tgaatgcgaa
    15961 ccggattgcc gcctccaaaa ctggtttctc caaccaatac ttcgcgggat ttaaaacaaa
    16021 accatgaatt ttccattgag gatttcatgg ttcaaaatta gataaaaaaa ccgaaaggat
    16081 taactattct ttgatttcaa ggttgtcgag tttttctttt gaaagtgcct catcatcgtt
    16141 gatgatggag ttgaattccg acgcttcggc ttcggcatcg agctccgaat tgtccggttg
    16201 cggaccttca ggtatttcat cggattcagg ttcaaatcca gcagggtatc tgccgctgtt
    16261 tgcttccagt tgcagccggg caaattcacg ggctcggcgt gattggtgaa tggctctcat
    16321 gtattttcct tcgagaaaca gcataatggc tatattctga tggacaactg cttttttcag
    16381 attgccggtg aaaacctgat tggcaatggt ggatttatgg gctgcataaa tgacaatggc
    16441 tgaccggttg acgacttttt ttgctttttc tttcattact ggctgtgcct gaacggatgc
    16501 acccatgaat aaaaaaacga atgaaaaaat gaccggtatt aatgcaatgc gtttcattgg
    16561 tttgaaattt tctgcaagat agtacttttt ttgaaaaacc ggttgtcatc aaattttata
    16621 ccagtgagta aaatctattg aagaatttga aacaatccga aaggttatta cacaattatt
    16681 ttatatttta gcactttgta tcagccgatg aaaaaactgt tgccactgtt atttttttta
    16741 tggtgctgtt ctgttgtgca ggctcagttt attctgtctg aagaagcccg gtttcaactg
    16801 aacccgcctg ccttttctga ttcatgtttg cctgccttaa gtatccccgg attatttccg
    16861 gttattgatc tggagccatc gcgcttggat ggggatgatc gcggaagctg gtttcgggaa
    16921 aaggtttttt acgatcattt tgtgaaaata aatgatggct cccgatatga attttttctt
    16981 gatcctgttt ttgatttcag ggctggaagc gaagataaaa atcagcttta taaaaacaca
    17041 cgtggctata tattgaatgg tcggattggt gaaaattttt ttgttcgctc tgagttttac
    17101 gaaacacaga caaaattgtc tgattatgct gcagcatggg tagataccat gaaattcatc
    17161 cctggaatga ttcgtatgaa accctttggt atcaacggtt ttgattatgg tgttgttttt
    17221 agtcagatca tgtggcagcc gtttaaaaac tatggtgttc gtttgggcta cgatcgtttt
    17281 cactttggca aaggttatcg cagcatgatt ctctcagatg cttcgcaggc atatccattt
    17341 ttgatgaata ctttgacgca tggaaaatgg agtttaaatc acaatatagc ttcgctgcat
    17401 aatcccgatt tcaacaatag actcaatgtt ccattatctg aaagtggcgt gtatcagcag
    17461 aaatggtttt cgtttacata tttgacatat agtccgaaaa gctggctgaa tctgggtttg
    17521 ttcgaatcag tcgtttttat gcctgccgat tccaccggca tttcttttta ccccatgtcc
    17581 atgttcccat tgcctttggc tcgtaccatt gccgtagaat ccaaagggct gcatcatgcc
    17641 atgattggct tccaggccga tgctacatgg gaagagcaat taatttttta tactcaaatt
    17701 gtagctgata aaattgactt ttcaaagctg aaatatcagc agggaattca gggagcaata
    17761 caattaggtg taagattgat tgtgccagaa ggaggatatg tctttagcga attcaatctg
    17821 gccaccaaca atgtttacac atcgtcgcat gcctggacgt cgtacacaag caatgatcag
    17881 ccactcgcgc atccgaccgg acagcagttc aaagaaatta tctgcggttt tcattggcgt
    17941 gaaaatccgt ttttgcttga tatcagtttc aataatatat gggcaggtgc attgcctgta
    18001 accgataatt accttcgagc cgatagttac accgatgtat tactaaccgg tttttacgag
    18061 cccatcaatc gtcccgatcg tgtgatgcat ctgggtttta cagtagcata tttcattaat
    18121 catgtcacta attttgctgt ctttgcaggc atgcattatc gcaactatca cagcacactt
    18181 tctgtgacac ccgataattc gctcacctac gaaattggac tccggcacat gatccgcaaa
    18241 caatattatg attttttcta gatgaaaatt gtctgtttgt caatattaat cctgattgta
    18301 ttatggctgc ctgcgcaggt ccccagttct ccttcggttg ttgcaatgga gatgaatgta
    18361 agtgcaatgc accggccatt caaagacagc attggaactg cacactcatc gttgaacaat
    18421 tttcaattgg tcaataatca aagttctgac actgagaaaa tatttttcat tgagccaatt
    18481 gcttcaacgg aaatcatgtt tggcagtgac tttgcacata tgtccggtgc aggagtcaaa
    18541 atgacttttg agaaaaataa attttcagca gtcatgttgc cgcaaagcga tttcgcagat
    18601 tatcctggtt tcgttggtga aaaatttgac agcatgaatg tatttccgta taagggaaaa
    18661 tcattgattt catcaggaaa tgcaagcgga gtactgtcgg ttcctttgta tatgaaatat
    18721 gaactgagca gatttttttc gcttgaaggc ggtaatggga aggcgtttct gggcgatggt
    18781 taccgatcga tgatgctctc cgatcaaaca gctgaatatc cttttgctag aattgttgcc
    18841 aatctgaagc agataaaata tatgcatcag atttcgcgtt ggcgacagga attgagcgat
    18901 ggaagcaatg aaacaaaatt cgccgctacg cattatctga gctggatgat tacaaaaaaa
    18961 ctaaattttt ctgttttcga atcggttatc tggaggtccg aagatagtgt caggcagcgg
    19021 ggtttcgaat ggttgtatct gaatcctgtc atgtttttcc gcccggccga atttgcattg
    19081 ggctcgcccg acaacaatgt gttaggtgga aatatttcat ggaatttatt ttcgaaaaca
    19141 aaagtatatg gacagtttgt actggatgaa ttttttattt ccgaaatcaa aaactggctt
    19201 aaacatcttt tgaaacctga tgatgccgca atacaatatg gggcgtgggt caataaacag
    19261 gcctttcagg tgggtgtaaa atcgatggat gttttcggaa tgaaaggttt taattgtctt
    19321 tccgaattca attatgcgcg tccatacatt ggcagtcacc gcgatccgat gctgagcaac
    19381 acgcatatga atcagccggt ggcgcatcct tttggtgcga atttttacga atttgttttt
    19441 cggtccgaat acaaaaaaac aacatggtcg gcgcatctga acatgatgta ttatctgacc
    19501 ggacttgatt ctgcaggcac acattgtgga cagaatctgc tgcaatcaac gtttgattca
    19561 ccactaaata atggaaatat tccggtgcag tattatggaa atgctgtcgg acagggcgtc
    19621 cggcacgatg ttgtattcat caatgcggag tttcgtaaga atttattttg gaacagtaac
    19681 atattaatct ggcgcatcgg tgctatgtat cgttttgata agtcagcatt gatcaatgct
    19741 tcggacaaat atatttatac cggaattttc ttcaatatgc ttccggatga atggaatatg
    19801 cgttgaaatc agaaattgaa aactgcaaaa gattgcagaa aagtataaat actggataac
    19861 cagataatca gatagattac tgatcctggc atcaatcgca tcatgaatgg ccattttcgt
    19921 tcgcgcacgg caaatttagg ttttggtctg attgcttcaa tgagccatat gataacaaga
    19981 ctgattatcc agattgggat gaggccgtat aaaagaacaa accatgtgag cgcccgcagg
    20041 gtagggaaat gtccatcgag aaaaataatt gttccgggtt ccttgcgcag aatagttgag
    20101 ttgacgagtt taaagaaatt cttgcggtca agcaatcgtc catcatcact gagatatgaa
    20161 aaattgagtt tatttgtgag gacaatattt gagtcttttg acaatctgaa ccaggcaatg
    20221 ggaaattcat tctccgacat aaaaatctgt tgtctgtttg gctctccggg ctgatgaata
    20281 accagtttgc cggattcagt aatgaatggt aatgtgtcac tgacatctgc tttgaaaagt
    20341 cgtctgctaa ttgaacccgc ttgcatggat gtttcgtccg attcaagaat cagtattgtt
    20401 ttctgattgc cgataatgtt gaaccagatg caattggttg gttttggttt gctgattttt
    20461 ttttcgctga aattgttttt gccgatcatc aacagcgtgt cgggatatgg tttttgtggt
    20521 accaggcctg agatagagta atcaaattgt ttttgaactt tgtgatggct tttgttcaga
    20581 attcccggga aatccggaaa taccgccctt tcatcgaaga tcagaaaatt ggttttgttt
    20641 ttcgaaccca gtaaaatcca tttttcgtct ttttgatgga aatcggtact gagaagaaaa
    20701 cgccatttat cgttttcggg ccatgctcca actatttcac agatacggtc aaaaaaacct
    20761 ggcagctcgt attttctgaa atcgacgact gtatcatcaa tggaggcaat cgatccgact
    20821 agtttttttt ggcgaccgaa aacaatttcg ggttggtttt tgatgtagtg aactgcttta
    20881 atatcggcag gggcagtgaa tataagtccg ggagctacca gcgagtcgaa atgaatgatt
    20941 ttaacgatgg agtcggaagc caatcctaca atggtcagcg aagaatcagg agctgataaa
    21001 acggctatca atgaatcgaa ctgatgcgaa atcgggaaag ggaaatgcac cacggattgt
    21061 ttttcgtggt ctgttttcca tagcccgctt gcgagaattt tattgtcttt gttttgcgaa
    21121 ataaagccaa taaatgcccc ctggtaatga atgccatagg gtattttgct ggagaagtag
    21181 gccgaatcag ggttggcgcc tgtcggatgt atcatgccat caccctgata cgcctctttc
    21241 cggattagta aaattatcca gagagcaaaa aacagaacaa tgactaaaat gtttagaatc
    21301 ctgacaattc gcatgggggc aaaggtaatg atttacgata aatgatttta gatattcgat
    21361 ttgcgatttg aaagacatcg aatgcatatt ggaaggacga cgcggtgtat gagttgtgca
    21421 gaccgctcca tcacataatc tgtcagattc accaatgacg gatcgccgtt tactgcttat
    21481 gacgagacct acttgacctc gacttcaaaa atactggcta ctgacgactc acaactgacc
    21541 actaattatt atctttgccg gctactgatg aagaaataca aatacgttat ttttgatctc
    21601 gatcgcacat tgtgggattt tgatcgggtg tcgcatgaag tgctttcaga gctgtttctg
    21661 gagcttgtac agccacatac tcaatattcg tttgaatatt ttcaccagaa atatgctgtt
    21721 atcaattctg gtctttggga acaataccgg cgccatgaaa ttaaaaagga agtgctgcgg
    21781 gtaaaacgat tttcgcttgc cctcgaagaa ataggactcg accggccatg gattgccaat
    21841 gaactggccg atgagtatgt gaagcgtacc gccgatcacg cttatttatt tccgggaacg
    21901 ttggaattgc tcagttatct aaaagagaaa gaatatattc tttctgttat gaccaatggt
    21961 tttaaagaag cgcaatatcc taaaattgcg cgcagcgggc tggggcctta ttttgaatat
    22021 cttttcattt cggaggaaat aggattcaat aagccggata tccgcatttt tgaatttgcg
    22081 ttgaagaaaa tggatgcaat gcccaatgag gtcatttttg tcggtgatga ttatgaggtc
    22141 gatatcgaag gagctgctgc tgccggcatg gatcaggtgt ttttcaatcc gcacacaggg
    22201 ccatctgata atggaaaacc tgcaacctac cgcatttcgg agctggctga gctgatgaaa
    22261 attttataac agctgtgcga agcagcagct gtgcgaagtt tccaatcaaa gctgtgcgaa
    22321 gttttcaact tcgcactact gctttaaaaa tcgcacggtt tcgaaaacat tgtcgccact
    22381 gagctgcagc agataaattc cggccggtaa ctgcgataca ttcagtgttg taaaaccatt
    22441 ttcaaaacag ctttgcatga gtgttctgcc tgaaagatcc attattatca acatgccatg
    22501 atcggctccc ctgaatccat taacctgtaa tatatccgtt gtcggattgg gataaacact
    22561 cagttttgga gcatctgtat tttctatatc aagtgttgta tgttgaaatt ttttgaaaaa
    22621 attccagatt tcgatgctgt atgaaatgtc gttgacgccc ggtataagcc aggtgtgatc
    22681 ggcatcgttg attttataaa accatacttc agaatttaaa tcaccgttta agtatttata
    22741 aagatccacg ctcatgctgt ctgcagctgt atttggcaat gctgtatgaa cagccggagt
    22801 gtcgcagttg ttgaagtttg cccagaaatt caccagctct tcggcatcat taccatatgc
    22861 attgccggta taggcaacgg ttccatcggc ggtgccgtga aaatgagcaa ctggtgttgc
    22921 cctgaaagga ttgcaggtgc atgaagctcc aatggttccg gcaacagaag caatggcaca
    22981 gatgcgcgtt ccaagctggc aggccagacg gttggacatg aaaccgccca tggagaaacc
    23041 agttgcgtaa acccgcgtca gatctatatt gtatatagct gcagtggaat caatcaatgc
    23101 acttataaat ccgatatcat ctactgttgg attcaatgtt atgcccatgt aactggcgcc
    23161 gctgttccat gctgtgccat atgggctgct catggcctgt ggatatagcg ttataaattg
    23221 tgcagaatcc gacaacaagt gtattccgat gttgctgaag ttggtcatgt tgtcgccgag
    23281 gccatgcaga cagattacca gcggaaccgg attggaaccg gtgtaggaac cagccacgaa
    23341 ttctttgtat tggcgcgaaa cacctcccac ctgaatggtt ttagtggtct gcgcaatcaa
    23401 tgaaaaagac aatacggaaa atacaatggc taacaatctg gttttcataa gcaaattttt
    23461 tcaaagataa atatttttgc tacaaatgtg cagaggatga atggttttaa atatacattc
    23521 cgtcttttct tcaaatcata tgctccgcat ttttttgtcc ccggggtgga gtttatcccg
    23581 ctgcctcctt cgatacgtcg caagccactt tttgaaaaag agcaatggat gcgacactgc
    23641 taatgaacac aaatgcttaa aattctgaaa atcaataaaa aagtttaatg aagttcccgg
    23701 tctccgcttg tccacaagct ttgccggtgg aagctggaat ctcatcttat actacagaca
    23761 tttttttgtt gattttcact gcctccttcg atacttcgca agccatttca caagaagcgc
    23821 tacatgagcg aaactcagga tgacagcgac ttaaagtgcc atgtgttatt caggttgcaa
    23881 aacgtgtttt gctggtgctt gtcgcgtaat gtcagcatga ctgcacgcgg gtctcaggat
    23941 gacagcgggc gggacatctt gagcttgacc tccctccttt tcgggtgctg cgcgcgtttt
    24001 acccggggta gcggaattta tcacgccata ggcgtgactt cttgccgcaa ccatcacgtg
    24061 aaacgtgata aatgggctac caatatgtaa gtccggagtt tactccgctg aggcggagga
    24121 ctatctgaag gtactccgta gcttctctga cggcttcgcc acttaaactt gttcttgtcc
    24181 tcgaccttaa cctgggcgcg cgtctcccgc ttgcaaccat gtaaaacagc ttgtcacgtt
    24241 ttacgtgatg gcaagctgtt ttacgttgac gcgtgaccat tgcctcaagc tccgcttgat
    24301 ctgcttttat gcattccgat ggcatggtgg atggcgtctt cgccaaagcg gcggcggatt
    24361 ttatcgagcg actgatagag gcgcgtgagc tcgggtgttt cgtcaaacag gtccagctgc
    24421 tggttcccgc ctatgagttc actgaatcgt attccgataa gtcgcaccct gagacgtctg
    24481 gagaacagtt tgccgaagat gtcttttacg ttttgcacca gcacatgatc gaaagctgtg
    24541 tatggaattc gtttttgcat ggtatgtgtt tcgaaatcgg catagcggat ttttaccgtc
    24601 acggtgccgg tcaggcggtt gtttttgcgc atgcggaatg ccagccgctc cgtcatggaa
    24661 gaaagtatgc gttccatttc ggcgcggtcg gttacatcag tttcaaatgt ggtttcggtg
    24721 ctcatcgatt tggcatcttc gtagggcacc acgggtgtat cgtcctgccc attggccttc
    24781 ttccagagca tgaggccgtt ggagcccagc acgcgggtaa gcagcaccgg tggcatgttg
    24841 ctgagtacgc caatattgac aatgcccatg gagcggagca gatgggccgt tttttttcca
    24901 agcatcggta ttttctgaat gggcagcgga tcgagaaagg gtttgatggc gggttgcgtt
    24961 atgtgaagct ctccattggg tttggcttcg ccggtagcga ttttgctcac ggttttattc
    25021 accgacaatc cgaatgaaat gggcagtccg gtttcattga tgatgcgttc gcgcagttcg
    25081 tgagaccatt tcagggtgcc gaaaaagcga tccatgccgg tcagatcgag ataaaattcg
    25141 tcgatactgc ttttttcgta taatggcgct ccttcgcgga tgatgtcggt taccatgcgc
    25201 gaatagtagg aatatcgctc catgtcgcca cggatgacaa ttgcatcgtt gcaaagcatg
    25261 cgcgccattt tcatgggcat gcccgaatgg acgccgaagg cccgggcctc gtagctgcac
    25321 cccgaaacga cagcccggtc acttccgccg cccaccatga caggtttccc aattaaagcg
    25381 ctgttctctt ttcgctctac cgaaacaaaa aacgtgtcaa ggtcaatatg taggacagag
    25441 cgatccatat catgattaga aaatagccaa attcaacgat aataatatag gcatattttt
    25501 gaaacgttct gacgattgtt gaaaaatatt tttctggcac ggattgcaaa ttcgcgccag
    25561 aaaaggataa aaaaatgtgg agacgttgaa tttcagcgtc tccacatatt ctttgaaata
    25621 aataaaatta tttcgcgtaa gagaccgcgc gggtttcgcg gataaccgta attttgattt
    25681 gaccgggata ggtcatggat tcctgaattc gtttgcttaa atcgagcgag agttgggtag
    25741 ccgcgccatc gctgattttt tcagcaccga cgattacacg caattcgcgt cctgcctgaa
    25801 tggcgtaggt tttctgcacg ccttcgtagc tcatggcaat ggcttcgaga tcattgaggc
    25861 ggttgatata gctttcgact acttcgcgac gtgctccggg acgtgcgccc gagatggcat
    25921 cgcaggcctg aacgataggt gcaatcaacg aagtcatttc gatgtcttcg tggtgagctc
    25981 caatggcgtt tacaatttca ggatgctcct tgtatttttc ggccagcttc atacctaatt
    26041 gtgcatgcgg cagatcgggc tcattatcgg gtacttttcc gatgtcgtgt aacaaacctg
    26101 cacgtttggc cattttggga ttgagtccca gctcagcagc catagtggcg caaaggttgg
    26161 ctacttcttt actgtgctgc aaaaggtttt ggccatagga tgagcgatac ttcatttttc
    26221 cgaccagacg catcagctcg tggttcatgt tgtgaatacc caggtcaatg agtgtttttt
    26281 taccggtttc gataatttcc tgttcaatat tggctttcac tttggcaacc acttcttcga
    26341 tgcgggccgg gtgaatacgt ccgtcggaca cgagtttttc gagtgcctgg cgggcaattt
    26401 cgcggcgtac aggatcgtat gctgacagaa tgatggtttc aggggtatcg tcgataacga
    26461 tttcaacacc tgtagctgct tcgagtgctt tgatgttccg accttcgcga cctattatac
    26521 gacctttgat ttcgtcgttg gcaatcggga atgaagagac ggcattttca ccggcatgat
    26581 cagtggcggt gcgctgaatg gtttcgatga taatttttct ggcttcgcgg tgggcattga
    26641 tctttgcttc ttcgacaatg tcctttacat ggctcatggc ctcaattttc gcttcttcct
    26701 gcagtgaatt taccaattgt gtacgcgctt catcagccga cattccagag acagcttcta
    26761 atttttcaac gctttctttg tgtaatttgc tgacatcttc gagcttttta ttgatgattt
    26821 cggtttgcgc tgtcagattg tctttaagag tttgagattc tttgtgtctg cgttgaattt
    26881 cttcgttgcg ttggttcagc gtcatttcgc gctgtttgag tttgttttcg atgcttagta
    26941 tccgtttgtt ctgctcctga ttacgttttt caatttcaga tttcatctga ataaacttct
    27001 ctttggcttc gaggattctt gtctgtttaa tcatatccgc ttctttattg gcgtcttcaa
    27061 gaagattttt tttcttcctt tcggcgcttt tgcggaaaat gaaagcccag agaagaattg
    27121 caatcacaag gaccacaact tccagtacta acattaacac attgttttcc atagttcttt
    27181 ttcgtttaag taaaaaaccc gcatagtcat cagggagttt caaacccccg ataaaaatgt
    27241 tttggagtca accagtgcat tcagacttcc aatgattcca cggaggaatt cctttcggat
    27301 ttggcctgtc ttagggcgct atgagtcttc tccgagattg attctgttga ggtttatcaa
    27361 ccataaaaac tgatgaacta cgcgggtcgt ttctttatga aaagaactat actgctttga
    27421 ccagcagttg gttaatttca gataatctcg aaagcaattc ttccttactg ccagaatctc
    27481 cgctattgtc tttttttgca agagctgcgg aagtgttaag tagaatcatg gccagaatgt
    27541 cctgcatatc cttgcccgga taatgcatgt tgaaatcctt tatgcggctg ttgattgatt
    27601 ctgttgcatt tctgattacc atttcatctt ctttttcaac agacagcgtg tattttctgt
    27661 tggccagcgt cacggttatt tttaccaatc cactcatcct aatcttccag cattaaaata
    27721 cacttatcta tttcgcgtat aagttcgttc agtttttttc tggttgattc agagtcattc
    27781 cggttttcca ccccttcagc aatctgacgt gcagtaagca atatttcacc gtttgtcaat
    27841 tgttgcttaa gattttcatt ttctgcctgc acccggcgac cttcctcttt cagaaactca
    27901 atttctttac ggtatagatt gagttgagca ataagctttc tgacattgtt gtcaatttca
    27961 gaaagtaact gattggtctc tttcatccgg aaagaatttg aattgcaaag atatgaataa
    28021 tttgccggaa atcaataaag tatttgattt aaaataaaaa aggcggccat atcggtcgcc
    28081 ttttctatgt aatacggatt gtaatttatt caacaacaat ctgtttggtc agtctgtttc
    28141 cgtttgacat cagagaaacg aaatagtttc ctgaagcaag gtcagagatg tcgatgcttt
    28201 ctttgtaata gttgcttgcg ttttgttcgc caagttcaac ttctttaaca actgcaccat
    28261 ttgaaccaat aatctgaact tttacgttgc tgtcaaactg gagagcatat tccataacaa
    28321 gcatgtctga agcaggattt ggtgaggtgg tgagtttcat tccctggaag aaattgtcat
    28381 tgatgccggc tgaacccatg tcaacaactg caaaaagagg aaggtttaca tcaagtgcac
    28441 cgcttgaacc atagctcagt gatacccaat agtaagcaaa aggatcctga ttgtagaagc
    28501 aatagttcat accattgtgg ttgccgggtt cgtcgcaata catgtaagct gtatcagtgg
    28561 caagacgcat tccttcgaag tttgaaacga tagagaaatc ttggttaacc cagagaggag
    28621 ctgagagggg aacatagttg aaaccgaggt ttgcccatgc ggtgtcaata gcgcttgctg
    28681 ccagttctac agtggcaaga gctgtgccgg aatacatatc cggtcccgga gcataagtgc
    28741 tggctgcaat aaccgtattg tcatcagcaa tgtcataaat tgaaagttca attttagaag
    28801 aggcgccgcc tgtcaggatg gttttaccgg caattccgat gataacaccg gtaatgctta
    28861 ctggctgatc gacaaaataa cccatggccc aatagtcaat agatgaggtg tcagaaggat
    28921 catagtttac accaaaccag tatcccagat cattgtcgct ggcgtcaaca agaccgtaaa
    28981 tgtggaaagc aggagttgga tctccaaaac aaggcaggta tccaagtgtc cagcccattg
    29041 tgtcgaatgg ggctttaacg ctttgtgttt caacgtattt tctgctttca agtaatttgg
    29101 agtccatttg ttttggggta agcaattgtg cgaaaagggt tgcactgatt gctacgagaa
    29161 cgaaagagag taatagcttt ttcatttttt gtgttttaat ttgacttgtt ttttgaggag
    29221 acaaatatag taaaataatg gttgcacgcc aaattatcat ttttttagaa tgactctaaa
    29281 tgtggcaacc atcattttaa tttattctac aggagcacag gtgcaaaaaa tattgcggtc
    29341 accataggca tcatcaatgc gggtaacagt gggccagtat tttagtgcat ctttgcgatg
    29401 gcaggtggct ttttcgcgcg aatagggttt gttccagttg tcggtggcca ccatttcaac
    29461 ggtatgcgga gcatttttca acacattgtt ttctttgtcg catttgcctt ctgcaacgtc
    29521 acggatttct tcgcgaatgg ccaacatagc atcaataaaa cggttcattt ccgacagtgg
    29581 ttcactttcc gtgggttcaa tcatcagtgt tccgtgcact ggaaatccga gggtgggagc
    29641 atggaatcca tagtccataa ggcgttttgc gatatcggtc tcagtaatgc ctgctttatg
    29701 aaaagggttg cagtcaacaa taaattcatg tgcgcagaag ccatgttcac ctttatataa
    29761 aatattgaaa tctggttcca gctttttcat cagataattg gcattcagaa tggcatattc
    29821 agtggccatt gtcagacctt cggctcccat tagtttgata taggcgtatg agattggaat
    29881 aacataggcg cttccgaacg gagatgcagc aacagctgaa atggcttttt cgccacctgt
    29941 tttcacgatc acgtggccgg gcaggaaagg cgacaggtgt tttgcaactc cgatggggcc
    30001 aacaccagga ccgccgccgc cgtgaggtat tgcaaatgtt ttatgcaggt tcagatggca
    30061 gacatcggcc ccgatgaatc ccgggctggt atatcccacc tgagcattca tgttggctcc
    30121 gtccatatac acctggccgc cgtgtgaatg aatgatatcc atgatttcct taatcgattc
    30181 ctcaaaaatt ccgtgcgtgg aaggatatgt aatcatcagt gccgcaaggt ctttggcgtg
    30241 ctcttcggct ttcagtttca gatcggcgat gtcaacgttt cctttttcat cggcttttac
    30301 aacaacaatc ttcataccgg ccatagctgc acttgcagga ttggtcccgt gtgcagagct
    30361 tgggatcaga cacacattac gggcggcatc gccacggctt tcgtgatatg cacgaatgac
    30421 catcagtccg gtatattcac ctgccgcacc actgttgggc tggaagctca ccttgtcgaa
    30481 accggtgatt tcggccagca gttgttcgag ttcatcgagt aaggtcagat aacctttggc
    30541 ctgatcagct ggaacaaatg gatggatatt gccgaattcg ggccaactta gataaaacag
    30601 ttctgttgcg gcattgagtt tcatggtgca actacccagc ggtatcatgg cgcggtcaag
    30661 agccagatct ttatttcgca gattggtgat gtaacgcatc atgtcggttt cgcttcggtg
    30721 cgaatgaaag acttcgtgtt tgaggaaatc gctcttacgg tcaaatgaag catcgaatgt
    30781 tcttatcttt tcgcattctg ccgggttgca aacgaatgga gtgtgttgtt ttcccgcagc
    30841 tgaagccaga atagtgataa tagtgttgat gtcgtcaaga gttgtggttt catcaatgct
    30901 gatgcctata agtttattgt cgatatagcg aaagttcatt tcattttgca gagcccgtgt
    30961 acggatatcg tccatgttta ctccgtcagg aatttcaatc tgaagggtat caaagaatgt
    31021 ttcattgatt tgtttgtacc ccgttttttg aatttcattg ctgagtaccc ctgccagaat
    31081 attgatgtgc cgtgcaattt ttttcagtcc gtcgggacca tgataaacgg cgtacatgcc
    31141 ggccataatg gcgagtaatg cttgcgcagt acaaatgttt gatgtggcgc gttcgcgttt
    31201 gatatgctgt tcgcgggtct gcaacgtcat gcggagggct ttgtttccga gtgcatcttt
    31261 cgaaataccg atgatgcggc cgggcatggt gcggacaaat tcttcttttg tcgccataaa
    31321 tgcagctgaa ggtccgccat atcccatagg tattccgaat ctttgtgtgg agccaattac
    31381 aatatcagcg ccccattcgg cgggaggagt cagcagggtc aaagccatca gatcggctgc
    31441 aacagcaagc atagagccat tggcatggac tttatcggca aaatcgctgt agttgtgaat
    31501 tttaccgtat tggtttggat actgaacaag agcgccaaag gttttatcgt taaaaacaaa
    31561 atctttcacg caatcaacca cgacttcaat tccgagtgga atagctcggg ttctgataac
    31621 ttcaagactt tggggcaata cgccatgaga aacgacgaat tggttggcac cagctttcac
    31681 tttgtcgcgt ttacgggtat tgtaggccat aatcatggct tcggcggcag cggtagcttc
    31741 gtcgagcaat gatgcatttg caatgggcat tccggtgaga tctgaaacga ctgtctggaa
    31801 attgagcagc gcttcgagcc gtccctggct gatttcggcc tgatatgggg tgtaagaagt
    31861 gtaccagccc gggctttcga gaatgttgcg caggataacg ccgggtacta tggtgccata
    31921 atatcccatc ccgatgaaag acttgaatat tttattctga gaaccaatat ttttgaggag
    31981 actctgggtt tcatattcat tgaggccagg tccgatattg agttttttgg gattaatgat
    32041 gcttttgggg attgtttttt caatcagttc ttcggctgat ttaactccaa tagcatggag
    32101 caatgtttgt ttttcttctt ccgaaggccc tatgtgtctg aaagtaaaat tattggtgat
    32161 cattggtctg ttgatttgtt tgattcgggc aaaattacct tattttttta gaactacttg
    32221 tgtattctgc tgatttttct cagtatattt gacaaaatat ttcatgctgc gcttttgcat
    32281 tgtttttttt gttataattc ttgttgtatg cggattagcg caggcacagg atacgcttta
    32341 cctcatgtca ggccggttga aaaccaatat ccatgttctg gaaatggatt catccaaaat
    32401 tgcttatgct ccgggtcgga cattaaaagt aaacaggcgt ggattggtca gaacgatgta
    32461 caaagaacgg caggatgttt ttgaaatcag atacgaagac ggaacacgag aattggctta
    32521 tatcatggat tcatcgggct acattataac gcctgagcag gccggatctt atgttgatgg
    32581 atgtcatgat gcgtttttgt attcgcataa tcgtattgtt ggccctgctt gttatcttat
    32641 tacattaggc agcatttttg ttttaccccc cattgctgtc attgcggttc cttgtgttta
    32701 tagtgcagct acaattatgt ttacccccga attcccggct gacagggtgg atgaagaaag
    32761 ggtcgataaa tattatatcc tcgggtacca ggataccaga aaaataaaaa aggtaaagtc
    32821 gtcaatgttt tttggaattg cggccattgt aacgggtttt gttattacgt tcactacaaa
    32881 ataatgcttg ttcgtttatt atcattactt ttgcatccca ataaagtaat cacaatcaat
    32941 acatggccct attcggaatt ttcggtaaag aaaaaaaaga aagtcttgat gatggtttga
    33001 aaaaaacccg ggaaagtgtg tttgctcgta tcacacgtgc tgttgccggc aaatcgaaag
    33061 tagatgatga tgtgcttgat aatctcgagg aagcacttat ttcatctgat gttggtgttg
    33121 aaactacact gaaaataatt gagcgtattc agcagcgtgt ttcacgtgat aaattcatga
    33181 gcacggatga acttaatcat attcttcgtg atgagatcgt tttgatgctt gatgaaaacc
    33241 gcagtcagag agcagttcag ccgggcgacg ttccaactta tgttattttg gttgtagggg
    33301 ttaatggtgt tggtaaaacc acaaccattg gaaagctggc ctatagattc aaaaatgcag
    33361 ggaaatctgt tgtgatcggt gccgccgata cttttcgtgc agctgcagtc gatcagctga
    33421 aagtctgggc cgaacgagct ggtgtcgata tcgttgaaaa aggcatgaat gccgatcctg
    33481 ccgctgttgc ttacgatact gccgcctttg caaaagcaaa agggtatcag gtggccatca
    33541 ttgacacagc cggacgtctt cataacaaaa tcaatctgat gaacgagttg ggaaaaataa
    33601 aaaaggtaat tcagaaagtt ttccccgatg gtccccagga agtgctgctc gtacttgatg
    33661 cctcaaccgg acagaatgca tttgaacagg caaaacagtt tactgctgtt actgatgtaa
    33721 acgcgctggc gttgactaag cttgatggaa cggccaaagg gggtgtggtg atcggcattt
    33781 ctgatcgttt taatattcct gttaaatata ttggcattgg tgaaaaaatt gatgacatac
    33841 agatttttga cagtgctgaa tttgttgaca gtctttttaa aagtggtcag tagtctgtag
    33901 tcagtagtca gtagccagtg gtcagtagcc agttgcctgt tataagtgat gagtgatgaa
    33961 tgttaagtga atttatactc acaactgacc actgactact aataaatggt ttttgtatga
    34021 aaaaaaacaa ggttggtatt ataaccatgg gttgtcccaa aaatctggtg gatagtgaaa
    34081 aacttgcatt tgcattgcaa aaagcgggtt ttgaagtgga acatgagcaa acggatgcgg
    34141 acattattat tgtaaatacc tgtggattta ttaatgatgc caaagaagaa tccattaatg
    34201 aaattctgaa tcacacggcc agaaaaaacg cagaaaataa tctgaaagtt attgttacag
    34261 gatgtttgtc tgagagatat cgtgatgaac tgaaaaatga aatccctgaa gcagatgcca
    34321 tatttggggt aaacgaatat gacagggtga ttgagtttgt gactggtaag tccgaaaatc
    34381 cgcaacagga gcgcatttta ggcactccat cgcattatgc atatttgaaa atttctgaag
    34441 gttgcaatcg gacttgtagt ttctgcgcga ttcctatgat acgtggaccg catgtttcgt
    34501 tgccggaaga agatattttg aaggaagcac agtggcttgc attgcatgga gtgaaggaat
    34561 taattgtgat tgctcaggat accgttagtt atggattgga tttatatggc gaacgtcgca
    34621 ttgcacggtt gcttcataag ctttctggca tagtgggtat tgagtggatt cgcctgatgt
    34681 acacttatcc ggctggattc cccgacgatc tgatagatga gattgcgcag aatccgaaaa
    34741 ttgtcaaata tgttgatctt ccattgcaac atatcagtac ctctataatg ggtagtatga
    34801 agcgatccat tgaccgtgaa tcgaccgaag ccctcatatc aaaaatcagg aatcgtattc
    34861 caggtgttgc catccgcagc acttttattg tgggttaccc aggtgagaaa gccagtgatt
    34921 atgctgagtt ggttcaattc atcaaagact ccagatttga gcggtttggt gtttttgctt
    34981 attcacccga agaaaacaca gcggcttttg aactgaaaga caatgttccg cataaaacaa
    35041 aaatgcagcg tatgtctggt ctgttgcggt atcacgaaaa agcttcaatc gactttaata
    35101 gcaagcttgt tggaaaaaca ttgtctgtta ttgtggatga atttaataag gaagaaaaat
    35161 gttactatgg ccggactgca attgatgcac cggaaattga caatcttgtg cgattgtttg
    35221 ttgatggtga taaagttctt cgggccggtg atattgttga tgcaaaggtg atttcttctg
    35281 gaatttttga tatcgattgt gaatgctgat ttggctgggt ttcaagtctg cagattgagt
    35341 tgcaaccatt tgttcagcgt atagataata aaaagttcat tcctgaaaaa tcgaaaaata
    35401 c
//
LOCUS       DAOPKE010000021        35199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1290_length_35199_cov_11.594013, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000021 DAOPKE010000000
VERSION     DAOPKE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_35199_cov_11.594013"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKY63_04455"
     CDS             complement(<1..428)
                     /locus_tag="PKY63_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99900.1"
                     /translation="MHIEEYIRMEMELNESMGKPIEDAAKARHFQSLYFMHDWMVLPG
                     YFTPAPDARLMLRSLEDLLEKDKKREEDGFTRRIRLGKLVKPGKGKKSKVVIVPTTTE
                     PKFYHDDSVTDEETTGGSGEGEEGEVIGKQQAQPQQGEGEA"
     gene            complement(464..6145)
                     /locus_tag="PKY63_04460"
     CDS             complement(464..6145)
                     /locus_tag="PKY63_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99901.1"
                     /translation="MKIKILRAVICIAFNSVLLTSFAQTQSMQLPIPSENAAVINFTD
                     PANPVLVNLPATQFNVGQNGPIGNPHIETNLRDAYPPINEFYLGQHPMFCQSVVNDAD
                     GNLLFFIVDNNIYNRFGEAFKDENDDPVYMIGGAFTVNSPLFDNVVNGEIIQNYRYSI
                     QEENIEQEAVLDPEICIFPLQNECYQYGLVCSAYAYGEQSKKAQEIIYRTITYVNDST
                     IILSDMTPLLNNNYINEYSDECFMGSTRDIAVTDYREEYDNYLLFLNYYGGLYVIEIS
                     GSGIMDLNNIVKVPSNVDITGDLAYWNFTIGSEMELIETISGGEKIYKVAYGLKNSHA
                     GYNNPFIIINEFSYNEPIQRLSAVRFDVPSFDNSDINNLIKGLEFSADGNILYYTYTG
                     QTGLYTYPDGVNLLSYLSTPPSYYQYSEIELGRDGNMYFLYSDVNENGGVARLTNPNS
                     PNCYNFTENVFPSISKIYTSYDHLNDEYIHKGSILLFADQIDGSDYTSYYNSLSQECC
                     RAHIYYGEWPSATKFDQNTDWTWEPGFDNNPFTSYDGKVMIENNVRVPSGKKLTLKNF
                     ELRFNQNAKLVLEPGATLILENSTLGAIDKCGLNQTWPGVEVQGNFLQSQLTPGAQAK
                     IILNNSTIENAVCGIVTRATDNSGNIFPGSAGGIILAKNSNFINCQRAVKITAYRNFN
                     PSTNQTIGNLSYFKNCTFELNNNLHSSLTPEDFVLMARVQNIRFFGCDFIDSRTNYGI
                     TRPDGIVAFDSKILVKGYCNSLQINPNPCPDFRPTTFTNLNYGIKASNALSLTNNVVV
                     DNCVFTHNWRDIYLRNADNSVITNNKHNINFTIQPFEHQAYGLYLEECTGYKVQNNEY
                     YSEFPYSTGCFVTNSGTSNNIIDKNYFHNLATGIQAEGENGSKGVIDRSFITTAVTLP
                     AYTGLQFKCNTFEANNIDIAVASGTIKRSQGYCSSSTETPAGNQFINSGSIVNIHAYS
                     TPSQPVTPFHYSYHNSTAPNTIPATSNVLSSSVTFHACSQTTFDYVTACPATGNLTIA
                     DLQVSIVQGKSALLNSLSLIDGGNTQALLDNVNSGISGGQLKDMLVSASPYLSDTVLL
                     ATIDRADALSPGNLKEVLIPNSPLTENVMTKVTDLNLPRGIMLQINNAQSGVSARAIV
                     DAQIESALDSIDLYEKELLNIYLNDTTGAGLSSLINFYSTLSDVESKKMVVQAAVADN
                     NISVSQAYLSQVPQISQEDIAFVDFYSLVNDLALDNRTILDIDSTEKILLEQIAQTNT
                     VVSSNAQVILDFLESVNHQELIQPLNFPDASYLNGAIVDNYLCGNNRIEGDTLVLVNT
                     SGEVVKAVTPVVSDANGSFRFDALEMYMLNKTDSFAIKTVSGFALAKTEFKTIPEWLS
                     NTSNELTLAPVNKQWADAYTHPDSVYNTATALDLSENLYVTGYCMTNSGVTEAHLVRY
                     APDGTRLWSGAYSYSNYNVPADVTTDASGNVYVVVNSTRTSSIRDIIVVKYDNLGNRL
                     WESVFPGTGLDEAIKIKATPDSEIVVVANAQNRVRLIKIKNNGNESWSNYYNSCVATN
                     LDVDNNGCIYISARTSDSQVNQGMNLLVYKPDGQLSFARETSRFASAITNSGFSRDIN
                     NNTILSGYSSTQSLCSKIDFAGNVVWEKLIPNEKVLSVTTDSAGNVYRGSFISGTYSF
                     TKCNGAASDLWTTTVKFPTGTELGTNLKLDLGADNSLFFGSTMRNYRARNDYFVGMID
                     PNSNLVWEQQYSLNSNSTDDFADIQVSNFNNVYLTGSSRFESVDSQGHNSKESMFVTV
                     KFATCPLTADLRSMISSGSDENVVSETDYLRVFPNPSDGNVFVEYNLSNSQTGTFTVY
                     TATGSPVLTVNLKTGLNTIAIDEKMLSEGVYYYQTIIGTDVFSGGKLLIIR"
     gene            complement(6152..7579)
                     /locus_tag="PKY63_04465"
     CDS             complement(6152..7579)
                     /locus_tag="PKY63_04465"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99902.1"
                     /translation="MKRIFFLIGFICFVTVSTHAQIMFQRHYGGSGDEYGSTCIQTSD
                     GGYIVTGSTDSYGLGGRDIYVVRTNEYGDTIWKKTCGGNGDDQATCIIETNDGGYAIT
                     GITSSFGAGSTDVYLIKINQNGDTIWTKTYGGSGAESGYCLLQTPTNDYIISGFTESY
                     SPVTSSIYIIKTDSNGDTLWAKTYYKQDVNTGYAIIQTVDNNYLVGITLSNTVSTHDY
                     MIAKIDSLGDTLWTKVYDKGANDWFSSVVELDGGDFVVSGFTNSLGYGGYDMFLARYN
                     SSGDEIWFKTYGGTEDDLGGGVIFTNDLSIINCGSTFSFGKGGEDFYLVKINLNGDTI
                     WTKTYGGIDNDYGGSIMQTTDGGLIIGGSSSSYGNGSEIFLIKTNPNGYAGIDQNYSI
                     VKEVKVFPNPNNGAFIIEQENSDASSSSLRIFDITGKQVYYRPDVFQNSMTENIAVPD
                     LCSGTYFLTISDEYHNAATKLTIIK"
     gene            complement(7633..8013)
                     /locus_tag="PKY63_04470"
     CDS             complement(7633..8013)
                     /locus_tag="PKY63_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99903.1"
                     /translation="MKINVLRTVICAAFIRIFYADYAQTQIMQLPIPSENASVINFTA
                     PANPVLTNLPVFPFNVGDQFGPPGNPHNEDNLRDAYPSINEYYLGQHPMYGQTVVNDA
                     DGNLLFFILDNNIYNRYGEAMIDV"
     gene            complement(8020..9459)
                     /locus_tag="PKY63_04475"
     CDS             complement(8020..9459)
                     /locus_tag="PKY63_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99904.1"
                     /translation="MKLLKISAITFTLILALIPAQTSAQIMFQRHYGGSGDDYGSTCI
                     QTSDGGYIVTGSTDSYGLGDWDIYVIKTDEYGDTIWTKTYGGSGYDVSCDIIVTYDGN
                     YAICGITDSYGYGNHDVYLIKIDQNGDTLWTKTYGGTGSDAGYSIIQTFDNGLVVAGN
                     TGSFSPVTASIYLIKTDETGDTLWTKTFYNQDVNTGYDVIETSDTGLLIGVTLSNTLL
                     THDYMLIKTNMNGDTLWTKLYDKGGNDWVSSVVELSGGDFVLSGRTNSVGYGGYDTFL
                     ARYNSIGDEIWFKTYGGTEDDLGGRLIVTNDSGIVLSCSSFSFGSGGSDVYVSKANLH
                     GDTLWTKTYGGIENDNGGSIMQTADGGLLIGGSSSSYGNGSEIFLIKTNSNGYAGIDQ
                     NCSIIKEVKIYPNPNNGVFTIEQENLDASSSALRIFDINGKLVYSLPHIFQHSMTEKI
                     EVPDLGSGVYFLTISDEYSNTATKLTINK"
     gene            complement(9492..11579)
                     /locus_tag="PKY63_04480"
     CDS             complement(9492..11579)
                     /locus_tag="PKY63_04480"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99905.1"
                     /translation="MAKKTSTDLSALNPASLDAHILKVISGERKFENVFQSVSRMILD
                     GPDKVEKVTINGRPTYDFKLFREGKKHIVGMYDEINSFVSFVKDAAEGGSSAEMAFVL
                     IGEPGNGKTFFVDFLSTKYREFISIPQNQRYTFRFNNVDLTGRYGKIDRIESQTYEDP
                     MVLAMNLFESLDDNKKYLSKLGYTDAQLDKLFKMHRPLGACTYFVYNQMKEHFGGDLG
                     EILKHIEIVPVPVSESRGTLTGKYAAKDKITSSSVDLLGDESISRLLHLADADNPYRF
                     DLRRGALARVAGGGIHFADEVFKNKRDLVQVYLGVIQNRSIEIEGFKWPLDTLIVATS
                     NNAEFARFQEEKEQAPIIDRCRLTYMSHNTNLHLQKLLTSYSLGSIEKTNFVGKKLHI
                     DPNLNQAISTAFILTRMPGNEKLNPVEMMKLAAGEVAGEKSMRTLAEVVNELNSDPDI
                     TRRFGQKGLGHRNLGRAIQILLEQSETQEGECMYAYDVFSAIDKVIIDYVQDQNDRIK
                     FMNDVKIARKLHRENVMTAIFNAYMNEANAIEKDVMNYVNMIIGIDAKNLGPNKIWTY
                     QDPQSKKLISIKIDESYINSVEERLGLKSEEQKQSFRTTITKIYGQKMNTDPNYNFMD
                     NNDLVKAVTDVRLKSDIAGAGSLVGALSNRNNEENNRLYNRMIDTMINVLGYCRTCAE
                     KTIEYFCTQDDTN"
     assembly_gap    12039..12138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12139..13854
                     /locus_tag="PKY63_04485"
     CDS             <12139..13854
                     /locus_tag="PKY63_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HPE99906.1"
                     /translation="LADMEGVASCYLSLSFINNRIADYPKALEQASNSLILYRKAGSL
                     EGMALAYNNLGNIYDNMSNYKQSLRYYHKALEIHSSANNKIGLARVYINLSIIYNKTN
                     KPGEAEELLNKAIEIGEETKDKRLLAIAYGNMGLMRENAGNYPDALEYYKLSLELKTE
                     LEDRHSIMISEINIGNTYLKMNQTDQALQHINKAVEIGNLLNAKRELIDAYSIQAQLY
                     ERRGNFSNAYAALKMKTALNDSIFGTAQLKHMADVDRKNEINQLESENQILRQKSIID
                     QLKLENNRFIRYYLLIGLSFAIVFIGLLIYQSGERRKSARRLDEVNKQLIKTNKQLKR
                     SESALAESNHAKDKFFSIISHDLRNPLASMVSFVRIMKRDYATLEQHERDALIEEFEK
                     IVNRTGNLLENLLMWSRSQTGRLNITPEKFKARNILDENAELHETLLRTKKIRISIEV
                     ENDNTEVFADKNMISTVVRNLLSNSIKFTPAGGKIELGFKHSGKNCVFHIKDSGIGID
                     ADKIPSLFSLGEVYVRTGTANEKGSGLGLVLCKEFVEKNHGEIWLESKTGEGTTFYFS
                     VPLFS"
     gene            complement(13827..14279)
                     /locus_tag="PKY63_04490"
     CDS             complement(13827..14279)
                     /locus_tag="PKY63_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99907.1"
                     /translation="MEKGKRSVVLTGLCILSMGGGLLGVLIGVLSIIDIELIRIFARI
                     PGYTSIYSLTYDAHFLYPYVKVVLYGLSFVGALQMLRLRKAGYFMYSVAQLVLLIVPY
                     LMWNLMPVVVFFTDLPDMIFSIAFIGAYSLYFFQGSFAADQEKSGTEK"
     gene            14433..14891
                     /locus_tag="PKY63_04495"
     CDS             14433..14891
                     /locus_tag="PKY63_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPE99908.1"
                     /translation="MKKLFLTTPLFNNDITTLFPRIILGLVFLFHGGQKMFGWFGGAG
                     LDATAQFMNINLDIPVFMGYMAAMTEFFGAIFLLLGLFTRISAFGLGITMFVAIITAH
                     PDAFLLSEGGMEYSLTLLFMSVLAFILGPGKISMDAVLFKKCISKKACRE"
     gene            15261..15334
                     /locus_tag="PKY63_04500"
     tRNA            15261..15334
                     /locus_tag="PKY63_04500"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15295..15297,aa:His,seq:gtg)
     gene            complement(15364..16173)
                     /locus_tag="PKY63_04505"
     CDS             complement(15364..16173)
                     /locus_tag="PKY63_04505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPE99909.1"
                     /translation="MLIASLAFAPVLALGLFIYLKDKYDREPMLWLLLAFVAGILTIA
                     PAIVIERLLEGRMGMGISPNFGSLMLNAFVVVALTEELCKFAVLRIVFYRKRFFSEPI
                     NGIVYAVMLSLGFAFAESVLHLVGAHNIYVTAIFRSFTAVPAHFMFAVFMGYFMGQAR
                     FTYFKHRFWLILTGLGLAVFAHGFYDLFLLAWWMPADFQFVSYGFLFAGIVIAVLFIN
                     KAQSRSPFMKRRKWLRTLEKIKERDFQEFLASQKKQMKNRRENMNNNDLAE"
     gene            16220..16492
                     /locus_tag="PKY63_04510"
     CDS             16220..16492
                     /locus_tag="PKY63_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE99910.1"
                     /translation="MKTIFSEKINIQEANKRGITSWPIWEKEASRFPASYDAKEECYI
                     LEGEFCIEAGNALFNFGPGDFVVFPADFECIWDIKKKVKKHYNFPL"
     gene            16492..17589
                     /locus_tag="PKY63_04515"
     CDS             16492..17589
                     /locus_tag="PKY63_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HPE99911.1"
                     /translation="MKTFHAGDKVRFISSKGSGIVTSVKKNIVHVLIEDGFEIPVEAS
                     DLVIIDQQEGKKNPFAKKDEFSKVQSNTVISKNKSFISKKSDNSTTTSSNSPNKENQK
                     TQNTSVSPATGIYLAFIPDNQDVLISGNMDIYLINYSKWQVAFHALKSDPGKGFASVS
                     CGLMDKGDAILIETVTEQDARHWNRLRLQLVCFDQKESQLFDSEIVDIDIKTSKFIKE
                     DIYTENPFFDELAHVIFVHDLTHPPLKGRKSDMIHENPEKKTNTVIDRYMTDKNTAEV
                     DLHIEKLRSDHKTMRKDEIKPVQLAFFRQCIDAAITKKLSRAVFIHGVGAGLLKKELT
                     DILKTYPDLEYEDASIMKYGIGATEVRFKNR"
     gene            17717..20104
                     /locus_tag="PKY63_04520"
     CDS             17717..20104
                     /locus_tag="PKY63_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPE99912.1"
                     /translation="MWNRIVNYILRRRALNLIVIGLLTVFMGYQALKVKMSYEYAQML
                     PMSDSTSIIYQDFKKQFEEDGSVLFVGIQDEKLKKLEVFNDWCNLSHDLKNIKGVGEV
                     MSIAHCLDLQKDTAAKKFKIVKLFETNPSSQAELDSILKKAYSMPFYDEMLFSKENGT
                     NVMMVYIDKKVLNSKERDKVLIDIKEPIEEFGVKHEIEMHFSGMPYIRTSISNLVQKE
                     LKVFMILALIVASIILLVFFRSLKALVFSITTVIIGVIWAVGTMVLMGFDITILSGII
                     PPILIIIGIENCIYLITKYHYEFAQHGNQAKSLARVVQRVGLATFLTNATTAVGFGSF
                     MVTSNPIMVEFGIIACVSILLEFLFTLILIPTMYSLFPPPKGRHIKHLDFTWLNGATR
                     FIINTIQYKRNLVLVAVIILLTGAGFGISLIENKGSVVDDVPKGDKLYTDMLFFEDNM
                     GGVLPLEITVDTKKKKGILNLNTLKKIDKFQTELKEIPAISKPLSVVEIVKFAKQSFF
                     NGRPDMYSLPNDNEKAFILSYLPDIDKGGEKNSILSSFIDTNYQVARISIRLKNVYTP
                     EIKEIKDTLESKLARIFPAEKYKTVVTGSAVVFEKGSNYLVSNLYGSLALAIVIIALL
                     IYILFSNVKMVFIAMTTNMIPLLVTAGLMGFLNVDLKPSTIIIYSIALGISVDAAIQF
                     LSRYRHQLKMTDWNIRESVLGALKETTSGMIFSGIVLVLGFGVFIFSRFGGTASLGYL
                     IGFTLLIALFSNLIILPSLILYMDKLIKNKEASKTLFDVEENDDPVLPPLNEEQQ"
     gene            20116..20979
                     /gene="rfbA"
                     /locus_tag="PKY63_04525"
     CDS             20116..20979
                     /gene="rfbA"
                     /locus_tag="PKY63_04525"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPE99913.1"
                     /translation="MKGIILAGGSGTRLYPITMAMSKQLMPIYDKPMIYYPLSVLMMA
                     GIRDILIISTPHDLPHFKHLLGDGSQIGCNFSYIEQQVPNGLAQAFVLGEQFIGSNNV
                     SLILGDNIFYSSGLHEKLHGMKKPDGGIVFAYHVSDPERYGVVEFDENMKAISIEEKP
                     SKPKSNYAVPGLYFYDNSVVEVAKNLAPSARGEYEITDVNKHYLHARKLSVEVLSRGT
                     AWLDTGTFSSLLQASQFVEVIESRQGLKIGCIEEIAFTEGFIDKKQLEIIAQPLLKSG
                     YGQYLLSLLQK"
     gene            20979..21926
                     /locus_tag="PKY63_04530"
     CDS             20979..21926
                     /locus_tag="PKY63_04530"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPE99914.1"
                     /translation="MISVLLTGGAGFIGSSLAEKLLENPDIFLVIVDDLSTGSIEKLP
                     IKHTERYKFIKADVNAYRDISEVFHAFRFDYVFHYSAMVGVKRTLENPVSVLLDLKSI
                     ENILNLSKNSTVKRVFFSSSSEVYGEPVEFPQNENTTPLNSKLPYAIVKNASEAFLKS
                     YHTEFGLPYTIFRFFNTYGPKQSKDFVISRFINLALKNKPLTIYGDGKQTRTFCYIDD
                     NVDATLNCFNQVIQNPAMHSDVYNIGNDNETYIDEVAKMIVRITGSSSELKYLPPLKE
                     GDMRRRLPDIGKMHKLLNRPLLPLEEGIKNILNDTRFII"
     gene            21934..22911
                     /locus_tag="PKY63_04535"
     CDS             21934..22911
                     /locus_tag="PKY63_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPE99915.1"
                     /translation="MKNTIQHYQEIFTERFNKINFVKFPHELYEPIDYALRAGGKRLR
                     PVLTIMICDFYGNNIEDAMNPAIAIETFHNFTLVHDDIMDNAPIRRGRPTVYNKYGTN
                     IGILSGDTMFTKAYEFVTLTREDKLPAVLKVFTKTAMEVCEGQQLDMNFETMPEVTME
                     EYMQMILLKTAVLMGACLKIGAILGNATAEQSEAIYRFGEHTGMVFQLQDDLLDTFGD
                     EKTFGKRIGGDILDCKKTYLYVNAMGKLKGDERQDFQQLYNNKTMDEVEKIIRVKEFL
                     INNNVIADCKAVMQNHHNLAMEALSETGLKADQKAILQEFAESMLVRDF"
     gene            23177..24691
                     /gene="gltX"
                     /locus_tag="PKY63_04540"
     CDS             23177..24691
                     /gene="gltX"
                     /locus_tag="PKY63_04540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPE99916.1"
                     /translation="MVRVRFAPSPTGPLHIGGVRTALYNCFFAKKNGGKLILRIEDTD
                     QTRFVEGAEKYIIESLTWSGIHFDEGPHVGGPFGPYKQSERKAMYHQYAMQLIESGNA
                     YYAFDTAEELDHLRKECESKKETFQYDCLSRKNLRNSLTMPESEVKNLLDSGTTFTIR
                     LKVPENSVIEFDDLIRGHISVKSENIDDKVLFKSDGMPTYHLANIVDDHLMEITHVIR
                     GEEWLPSAPLHVLLYRFLGWERPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPMNWT
                     DPKSGEQSSGYRESGYFPEAFINMLALLGWNPGDEREIMGIEEMSQAFSLDHVSKSGA
                     KFDPVKARWFNHQYLMKASIEDISEEFESLLKQQNISIPDKQKLNTITGLLRDRVDFV
                     KDLPEAGRFFFEAPKLFNEADLKKHSTPDSGNWLNALKVVFENTDWSAAAIDTAIATF
                     IKEGSLPAGKIYNIIRIAMVGGSSGPHLADILLLLGKEETLKRVDAMIQFLTKH"
     gene            24772..26304
                     /locus_tag="PKY63_04545"
     CDS             24772..26304
                     /locus_tag="PKY63_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPE99917.1"
                     /translation="MPIIGTLIKSAIELKNMMPVEKQKPSAITQQKKVLKKLIRKAQL
                     TAFGEHYDFAGALKQDDLLSAFKQSVPIHDYNKMHNDWWYRTLTGEAYVSWPGRVKYF
                     ALSSGTSEATSKYIPVTPEMLRAIKRTSIRTLVASARYDIPTDFFKRGILMLGGSTHL
                     QFNGTYYEGDLSGITTGNIPFWFQHFYKPGKRISRERNWETKIEEIVRKAPEWDIGAI
                     VGVPAWFQILLTKIIERYNLNNIHEMWPNLSVFTHGGVSFAPYAKSFEKLLGKPMIYI
                     ETYLASEGYIAYQDDPESDSMALVLDNGLFFEFVPFNDKNFDNEGNIQTNATAYTIDE
                     VEEGREYALLISSVAGAWRYLIGDVVKFTNLSKAQIHIVGRTKHYLSVCGEHLSQENM
                     NRAIEMLEHDYNISVPEFTVAGLPHDNMFQHKWWLGTNDSIDPEIAAKAIDNNLKQLN
                     DDYRVERIAAIRKVEVEVLPLNVFYDYMASKGKIGAQVKFPRVMKKHLPDWLEFLNNL
                     KK"
     gene            26316..26951
                     /locus_tag="PKY63_04550"
     CDS             26316..26951
                     /locus_tag="PKY63_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPE99918.1"
                     /translation="MLGIFIEGILLGLVLSITLGPAFFTIIQTSIDRGFKSAFLIAIG
                     VATSDLFIITISYLGLSAVIESGKNQIYAGIVGGVILVMYGVYTFFKKPEILKRRSSR
                     NTGVARETKPITFLAKGFFLNIANPFIILFWLTIIGYISQIAEYGKLEETATVFLSGT
                     ILTVFSMDVLKSFIGYKIKKYLRPRIQLLINRIVGFSLVVFGIILIIRLLV"
     gene            26962..27225
                     /locus_tag="PKY63_04555"
     CDS             26962..27225
                     /locus_tag="PKY63_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99919.1"
                     /translation="MKKRISYLCTNSGPMKPANKYFRVLLVAFSAAALFLTSNSCKNR
                     QQQSKYGPPSDTYQDQPVTKYGVITDTIGPVQTKYGVPVPENE"
     gene            complement(27227..27847)
                     /locus_tag="PKY63_04560"
     CDS             complement(27227..27847)
                     /locus_tag="PKY63_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HPE99920.1"
                     /translation="MSKKKNNFYVVWEGRFPGIYPDWDKCYEQISGFPGAKYKGFVTR
                     EEAEQAYLCGFEAGVKSVKRKQIDHSLRPQEVCICVDAACSGNPGVMEYQGVWCQDGQ
                     LIFREGPFKDATNNIGEFIALVHGLSWLKKNNMPFPLYSDSATAMAWLRNKKAKTKLE
                     RTSENVRVFQLLQRAEEWLKENPVHNEVRKWQTDVWGEIPADFGRK"
     gene            complement(27828..29018)
                     /locus_tag="PKY63_04565"
     CDS             complement(27828..29018)
                     /locus_tag="PKY63_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPE99921.1"
                     /translation="MPTTLYKYTFKTLAGLEPLLEKELLDAGCTNPEPVKRGFMFEAD
                     EAMMYRLNYTSRFGIRLLVEVLRFRFADKDEFLKNIRAFEWDTYVGAEKTIAVDAVVT
                     ASPIFNNTHFVELLTKDGVVDFFRNRCGLRPSVDKDNPDLLINIYINHDECVVSVDSS
                     GKSLHKRGYRIASHVAPLNEVLAAGIIGLSGWKMDCDFIDPMCGSGTILIEAAMMANN
                     IPAGYYRNDFGFMHWQTFKPAMFERVKKEAIAQTKEFEFNIIGSDRSDDSLKAAHTNI
                     RKARLDRDIELRRMNFFDIAPDSPAHIVFNPPYDARLPIENSRDYYKKIGDTIKNQLQ
                     GSKVWMLVGNIDLWKSIGLKATLKIPLLNGMIDSRLLCFEAYQGSRRSAAGQNQHPDV
                     EEKE"
     gene            complement(29114..29584)
                     /locus_tag="PKY63_04570"
     CDS             complement(29114..29584)
                     /locus_tag="PKY63_04570"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HPE99922.1"
                     /translation="MKKIYILLALIGLMAASCADDGDGTPTIINPSAKMTCKIDGVQW
                     TALTRVTTNTAGGFSINGSALQSDALDITIVGETTGTYTLGTMQFNFTASYSPVASNT
                     DSLYTAINGTVVLSEIDQTNRRVSGTFNFNAINVKNNALNKSITDGVFTSLAYN"
     gene            complement(29714..30058)
                     /locus_tag="PKY63_04575"
     CDS             complement(29714..30058)
                     /locus_tag="PKY63_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE99923.1"
                     /translation="MNKVELIGRVGKDPDVRETKTGKKMLFFSLATNESYMNAMGDTV
                     QNTLWHRIVLWSDVVSTCEDIVKKGHCLYIAGKLNNRNWVDKDGQKRTITEIVGQIVE
                     QRVPGQDMGVVG"
     gene            complement(30228..31784)
                     /locus_tag="PKY63_04580"
     CDS             complement(30228..31784)
                     /locus_tag="PKY63_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPE99924.1"
                     /translation="MRTFVSALVPLLFFCFFSLQSFAQEKKKILLKSADFLEYNRPVN
                     PDVQFLKGNVVFQHENSLLYCDSALLYITKNAMDAYGNVHIRVSDATQIYGEKLHYDG
                     NEKKAIMEQDVELIDNNIVLHTQKLYYDLENGIGYYLDSAEIQDPHNTLVSKHGYYYS
                     GKKEFFFRHGVVVTGNNFKMISDSLMYNTITEQIDFYGNTHIQSDSTDIYCEYGWYNT
                     LTDQTKLFNRAKLINGSSVLEGDTLYYDKKLGLGKARRNMVFRDTLENVMLLAHSGDF
                     SEKDSSVLATDSAVFITWEGTDSLYLHGDTLFTTKDSLLGREIQAYYGVRFYRFDMQG
                     VCDSLYYCEKDSLMRMFENPCLWSDSTQMTAEYIQFTIDRNEIRELYMKDNAFIVSMY
                     EELDFQQVKGVQMNGYFENNNIDHMLVTGNAEAIYFIEDENGAKTGINKSSSASMDLF
                     FENSEVVAINMIGSPQGTIYPNKDLGSDQRKLAGFEWRAGLRPLNKNDIFRKAAPVNT
                     GGVVRGNANP"
     gene            complement(31781..33277)
                     /locus_tag="PKY63_04585"
     CDS             complement(31781..33277)
                     /locus_tag="PKY63_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HPE99925.1"
                     /translation="MKKVFLIIVMLFSMQCMVFAQLPVKESDVPEVVKSKLPNLFPGV
                     TGVQWTRHNTDYHAAFSYDNIKVVVKFAESGRWINSESEYEIEDCPRSMQKHVTTSFA
                     NTKVVRILLTENKDISEYNVEMLDTLTKQPLFAMYDISGAFVRKSDSKGNDIDLQLQG
                     ASERGKLAVHPKELPSAINSYVIINYSQYSIRESYIVNNEKYQNAYYVVLGKSDDKVP
                     VELWFDFQGTPIAGANKPVSDSGDQKDQDKGQKKQTRTPFPQSKVPAAAVQYFTKKEP
                     KAEEVRWDTLGLHYVASYYNPIRSTENRMHFDKKGAFVMSVVVLNPKDLLPLAQNYLM
                     DNYPDLEIESAENLVYADKKKYTLVKLYGPTWMNDPMVFHEIYFSTSGRLEKEVLANF
                     IDAEDVYLREKRENQHAAFNEVTAEDDLSLEEGNNLDGQSVTHKELPSQSIKYIAENY
                     PGWSFTEGVIISDENVLKYSVFIKRDGYREHKRLLFDMKGSFLKEEDL"
     gene            complement(33283..34569)
                     /locus_tag="PKY63_04590"
     CDS             complement(33283..34569)
                     /locus_tag="PKY63_04590"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPE99926.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDALAPQYDIIARFQGGPNAGHTII
                     FNSRKFVLHSVPSGIFRSNILNIIGNGVVLDPFIFFQEIDAIREAGFDPSSNLLISRS
                     AHFIMPSHRLLDAVNEAAMGEKKIGSTLKGIGPCYTDKYKRSGLRVGELEHNGFQTEY
                     ESRREIHMKLIAASGVDYRGILLDGLPFDEYEQEWLKAAHKMKQFRIAATEIEINKSM
                     AAGKKVLAEGAQGTLLDVDFGSYPFVTSSNTMTGGVCTGLGVAPSHVGKVFGIFKAYC
                     TRVGNGPFPTELFDDMGELLRKNGNEFGATTGRPRRTGWLDMPALRYSVMLNGVTDLI
                     MTKADVLTGLETIKVCDAYKCADKLYDMPDIPAGCKPDLIIKEMNGWSADITTTTQAN
                     QLPPALTEYSRFVEKSAGAHLSYISTGPDREQIIKL"
     gene            complement(34601..35098)
                     /locus_tag="PKY63_04595"
     CDS             complement(34601..35098)
                     /locus_tag="PKY63_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPE99927.1"
                     /translation="MEKNAEIQQAVDNYDAVKQIFTEYLNRTKHRKTPERYSILKEIY
                     TRPGHFDIETLYIRMKNNRYRVSRATLYNTMELLLESGLVVKHQFGQSSAQYEKCLCF
                     QPHDHAICLKCRKVIEFCEPRVGEIEKTLLQTFGFRASSHSLVFYGICEDCSKDGQAD
                     FEKKD"
BASE COUNT        10540 a         7617 c         6831 g        10111 t
ORIGIN      
        1 gcttcgcctt cgccctgctg gggctgtgcc tgttgtttgc cgattacttc gccttcttca
       61 ccttcgcccg agccacctgt ggtttcttca tctgtcacgc tgtcatcatg atagaatttg
      121 ggctctgtag ttgtcggaac aatcaccact ttgcttttct tgcctttgcc gggcttcact
      181 aattttccaa gccggatgcg gcgtgtaaag ccatcttctt cgcgcttttt gtctttctcg
      241 agcaggtctt caagtgagcg aagcataagc cgtgcatcgg gcgccggagt aaaatagccg
      301 ggtaacacca tccagtcatg catgaaatac agcgactgaa aatgtcgtgc tttcgcagca
      361 tcctcaatcg gtttccccat ggattcattc agctccatct ccattcggat gtattcctct
      421 atatgcattg ttgaatcatt tgacataatg tagaaacaaa gttttaccta ataatcagta
      481 gctttccacc tgaaaacaca tctgttccaa tgatagtctg atagtaatag acaccttctg
      541 acagcatttt ttcgtcaatg gcgattgtgt tcaatccggt tttcagattg actgtaagca
      601 ccggactacc tgttgcagta taaacagtga atgtgcccgt ctgactattt gacaaattgt
      661 attcgacaaa cacattgcca tcggaaggat tcgggaatac acgcagatag tcggtttccg
      721 atacaacatt ttcatctgaa ccagagctta tcatagatct taaatcggct gttagcgggc
      781 atgttgcaaa ttttacagtc acaaacatgc tctcttttga attatgaccc tggctgtcga
      841 cggactcaaa tctgcttgat ccagtcagat aaacattgtt gaaattactt acctgaatat
      901 cggcgaaatc atcagtagaa ttggaattca acgaatattg ctgttcccaa acgagattgg
      961 aatttggatc aatcatccca acaaaataat cgttgcgggc gcggtaatta cgcattgtac
     1021 ttccaaagaa caagctgttg tcagcaccaa gatcaagttt cagatttgtt ccaagctctg
     1081 tgccggtagg aaatttaaca gttgttgtcc ataaatcact tgcagcacca ttgcatttgg
     1141 taaatgaata tgttccacta ataaaagagc ctctgtacac atttccagct gaatcagttg
     1201 tgacagaaag aactttttcg ttcggaatca gtttttccca aacaacattg cctgcaaaat
     1261 cgatttttga acaaagtgat tgtgtagacg aatatccgct cagaatggtg ttgttgttta
     1321 tatcgcgact gaatccagag ttagtaatgg cacttgcaaa ccgcgatgtt tcgcgtgcaa
     1381 aactcagctg accatccggt ttgtaaacca gcagattcat tccctgatta acctggctat
     1441 ccgatgttct tgccgaaata taaatgcagc cattattatc gacatcgaga ttggttgcta
     1501 cacaactatt gtaataattt gaccaggatt catttccgtt gtttttaatt tttatcagtc
     1561 gaactctgtt ctgggcattg gcaacaacaa caatttcgct atccggagtt gctttaattt
     1621 tgattgcttc gtcaagacca gtgcccggaa aaactgattc ccacaagcgg ttaccaaggt
     1681 tgtcatattt tacgacaata atatcgcgaa tggatgaagt tctggttgaa tttacaacaa
     1741 cataaacatt gcctgaagca tcggttgtta catcagcagg cacgttgtaa tttgaatagc
     1801 tgtatgctcc gctccagagt cttgttccat ctggcgcata tcggacaaga tgtgcttcag
     1861 tgactccgga attggtcata caatatcctg tcacatacaa attctcgctc aaatcaagtg
     1921 ccgttgccgt attgtaaacg ctgtcaggat gggtgtatgc atctgcccat tgtttgttta
     1981 ccggagctaa agtaagttca ttggacgtat ttgacaacca ttcgggaatt gtcttaaatt
     2041 ctgtttttgc caatgcaaag ccactcacag tttttattgc aaatgaatcc gttttattca
     2101 acatatacat ttctaatgca tcaaaacgga aacttccatt agcatcactt actacaggcg
     2161 taacggcttt aacgacttct cccgaagtat ttaccagtac aagggtatct ccttcaattc
     2221 ggttgttacc gcaaagataa ttgtcgacaa tggctccgtt cagatagctg gcatcgggaa
     2281 aattaagagg ttgaatgagt tcctgatgat taactgattc aagaaaatca agtattactt
     2341 gcgcgttact tgaaacaact gtattggttt gagcaatctg ttccaacaga attttttcag
     2401 tagagtcaat atcgagaatt gttctgttat ctaatgccag atcattcacc agtgaataga
     2461 aatcaacaaa tgcaatatct tcctgagata tttgtggaac ttgtgaaagg tatgcctgtg
     2521 aaactgaaat attgttgtct gctacagctg cctgaacgac catcttcttt gactcaacat
     2581 ctgataaagt tgaatagaaa tttatcaaag atgataatcc tgctcccgtt gtatcattca
     2641 gataaatatt cagcagttcc ttttcataca gatcaatact gtctaaagca gattcaattt
     2701 gggcatcaac aatggcacgc gctgaaacac ctgattgtgc attattgatc tgcaacatga
     2761 tccctcgggg cagattgaga tcagttactt ttgtcataac attttcggtc aacggcgagt
     2821 ttggaataag tacttctttc agattgcccg gcgacaatgc gtcagctctg tcaatggttg
     2881 ccagaagcac tgtgtcagaa agatacggag atgcagaaac aagcatgtct tttaactggc
     2941 cgccactgat tcctgaattg acattgtcaa gcaaagcctg cgtatttcca ccatcaatca
     3001 atgacaacga attcaacagg gcacttttcc cttgaactat tgatacctga agatctgcaa
     3061 ttgtcagatt accagttgcc ggacaggctg ttacataatc aaaagttgtt tgtgaacagg
     3121 catgaaacgt cacacttgaa ctcaacacat tgcttgttgc aggaatggtg tttggggcag
     3181 ttgaattatg gtaactgtag tgaaaaggag ttaccggctg agaaggcgta gaatacgcat
     3241 gaatgtttac aattgatccg gaattgataa actgattacc tgcaggagtt tcagtgctgc
     3301 tgctacaata cccctggctc ctttttattg tccctgaagc aaccgctata tcaatgttat
     3361 ttgcctcaaa agtattgcat ttgaattgca agcctgtgta tgccggcaat gtaacagctg
     3421 tagttataaa agaacggtca atgacacctt ttgatccatt ttctccttct gcttgtattc
     3481 ctgttgccag attatggaaa taattcttgt cgataatatt attacttgtg ccggaattgg
     3541 taacaaaaca acctgtagaa tatggaaatt ccgaatagta ctcattgttt tgaactttat
     3601 agccagtgca ttcttcaaga tacaagccat aagcctgatg ctcaaaaggc tgaatggtaa
     3661 aattaatatt gtgtttattg tttgtaataa cagaattgtc agcgtttcta agataaatat
     3721 ctctccaatt gtgcgtaaat acacaattgt ctacaacaac attattagtt aatgataatg
     3781 cattgcttgc tttaattccg taatttagat tcgtaaatgt tgttggtctg aagtcaggac
     3841 atggattggg atttatttga agggaattgc aatatccctt taccagaatt tttgaatcaa
     3901 aagcaacaat accatcaggt ctggttattc catagttcgt tcttgaatca ataaaatcac
     3961 agccaaagaa acggatattc tgaacacgag ccattaatac aaaatcctct ggggtcaaag
     4021 atgaatgaag attattattt agttcaaacg tgcagttttt gaaatatgac aaattcccta
     4081 tcgtttggtt tgttgatgga ttgaaatttc tgtatgcagt tattttaaca gctctctggc
     4141 aattaatgaa gttactgttt tttgcaagaa ttattccgcc agcactacct ggaaaaatat
     4201 ttcctgaatt atcagttgct ctggttacaa ttccacaaac tgcattttca atggttgaat
     4261 tatttaaaat aatttttgcc tgagcccctg gagtaagctg actctgcaag aaatttccct
     4321 gtacttcaac acccggccat gtctgattaa gaccgcattt atcaatagct cccagtgtac
     4381 tgttttcaag aattagagtg gctccgggct ctaaaaccag ttttgcattt tgattaaacc
     4441 tgagttcaaa gtttttcaaa gtaagttttt tccctgatgg aacacgaaca ttattttcga
     4501 tcataacttt tccatcgtaa gatgtaaatg gattgttatc aaatccgggt tcccaggtcc
     4561 aatcggtatt ctgatcgaat ttggttgcgc ttggccactc cccatagtaa atatgtgccc
     4621 gacaacattc ttgtgataat gagttgtaat atgatgtgta atcactccca tcgatttgat
     4681 ctgcaaaaag caatattgag cctttgtgta tatattcatc attcagatgg tcatacgagg
     4741 tatatatttt acttattgac gggaacacat tttcagtaaa attgtagcaa ttgggactat
     4801 tcggattcgt aagacgagca acaccaccat tttcattaac atcagaataa agaaaataca
     4861 tattaccatc ccggcctaac tcaatttcgg aatactgata ataagaagga ggagtactta
     4921 aataacttaa taggttaaca ccatctggat aagtataaag tcctgtttga ccggtatatg
     4981 tataatagag tatatttcca tccgctgaaa attcaagccc tttaattaaa ttatttatgt
     5041 cactattatc aaatgatggc acatcaaatc ttacagcact tagtctctgt ataggctcat
     5101 tataactgaa ctcatttata attataaatg gattattata acccgcatga gaattcttaa
     5161 gcccgtaagc tactttgtat attttttctc cccctgagat tgtttcaatc aactccattt
     5221 ctgagccaat cgtaaaattc caatacgcta aatcgccagt tatatcaaca ttacttggga
     5281 ctttaacgat attattcaaa tccattatac cagagccact tatttcgata acgtaaagac
     5341 ctccataata attgagaaaa agcagatagt tgtcatattc ttcccgataa tcggtaacag
     5401 caatatctct ggtggagccc ataaaacact catcactata ttcattgata taattattgt
     5461 taagcaacgg ggtcatgtct gatagaataa tagtagaatc atttacatag gtaattgttc
     5521 tatatataat ctcttgtgcc tttttacttt gttctccata agcataagca gagcaaacaa
     5581 ggccgtattg ataacattca ttttgtaatg ggaaaataca aatttcaggg tccaaaacag
     5641 cctcctgttc aatattctct tcttgtatac tatagcggta gttttgaatg atttcaccat
     5701 taacgacatt atcaaaaaga ggagaattaa cggtaaaagc acctcctatc atataaacag
     5761 ggtcatcatt ttcgtctttg aaagcttccc caaaacggtt gtaaatatta ttatccacaa
     5821 taaaaaacag cagattcccg tcggcatcgt ttaccacaga ttgacaaaac atggggtgct
     5881 gcccgaggta gaattcgtta attgggggat aggcatctcg caaatttgtt tcaatatgtg
     5941 gattaccaat tggtccgttt tgcccgacat taaattgagt tgcaggtaga ttcaccagca
     6001 ccggattggc cggatcggta aagttgatca ccgcagcgtt ttccgacgga atcggaagct
     6061 gcatgctttg cgtctgggca aaactggtaa gaagaacgct gttaaatgca atacaaatga
     6121 ctgctctaag aatcttgatt ttcatacgga tttattttat tattgtgagt ttggttgctg
     6181 cattgtgata ttcatcggaa atagtcagaa aatacgttcc tgaacacaga tcgggtaccg
     6241 ctatgttttc cgtcatggag ttctgaaaca catcgggacg atagtaaacc tgtttgccgg
     6301 ttatatcaaa aatccgaagg gatgaagaag atgcatccga gttttcctgt tcaataataa
     6361 acgctccgtt gttgggattt ggaaacacct ttacttcttt tacaatgctg tagttttgat
     6421 caattcctgc atatccgttg ggattggttt taatcagaaa tatttccgat ccgtttccgt
     6481 agctgcttga tgaacccccg atgatcagcc cgccgtctgt tgtttgcatg atggatcctc
     6541 cataatcatt gtcaattccc ccatatgttt ttgtccatat tgtgtccccg ttcaaattta
     6601 ttttaacaag ataaaaatcc tcaccccctt ttccaaagct aaatgttgaa ccacaattaa
     6661 tgatgctcaa gtcatttgta aaaatcaccc ctccacctaa gtcatcttct gtgccgccat
     6721 aggtcttaaa ccagatttcg tctccgctac tgttatagcg agctaagaac atatcatacc
     6781 ctccatatcc taaactattt gtaaaaccag aaacaacaaa atcgccaccg tctaactcaa
     6841 ctacagaaga aaaccagtca ttagcacctt tgtcatatac ttttgtccaa agtgtatccc
     6901 caagtgaatc aatttttgca atcatgtaat cgtgagttga aactgtattt gacaacgtta
     6961 ttcctacaag atagttattg tcgacagttt ggattatcgc ataaccagtg tttacatcct
     7021 gtttataata tgtttttgcc caaagtgtat ccccattaga gtccgtttta attatgtata
     7081 tacttgatgt aactggacta taactttcgg tgaatccaga aataatataa tcattagtag
     7141 gtgtttgcaa taaacaatat ccagactctg caccactacc accataagtc tttgtccata
     7201 ttgtatctcc attttgattt atcttaatta ggtatacgtc agtgcttcct gcgccgaaac
     7261 ttgatgttat gccggtaata gcataacctc catcatttgt ttcaattata caggtggctt
     7321 gatcatcacc gtttccacca caagtctttt tccatatcgt gtcgccatat tcatttgttc
     7381 taacaacata tatatcacgt cctccaagtc cataactgtc tgtagatcca gttacaatgt
     7441 atcctccgtc ggaggtttga atacaggtgc ttccgtattc atcaccactt cccccataat
     7501 gtcgttggaa cataatttgg gcgtgagttg aaacagtaac aaaacaaata aacccaatga
     7561 gaaaaaatat gcgtttcata atgaaagatt tttgtgcaat ttaccaattc tgttcaaatg
     7621 attttttatt cattaaacat caatcatcgc ctccccatat ctattgtaaa tattgttgtc
     7681 caaaataaaa aacaacaagt tgccatctgc atcatttacc actgtttgcc cgtacatggg
     7741 gtgctgtccg aggtagtatt cgttaatgga gggataggca tctcgtaagt tatcctcatt
     7801 gtgaggatta cctggagggc caaattgatc cccaacatta aacgggaata caggcaggtt
     7861 tgtcagtaca ggattggctg gggcggtaaa attgattacc gaggcatttt ccgaaggaat
     7921 tgggagctgc atgatttgtg tctgagcata atcagcataa aaaatcctaa taaacgcggc
     7981 acaaatgaca gttctaagaa cgttgatttt cataagaggt tatttattaa ttgttagttt
     8041 ggttgctgta ttggaatatt catcagaaat tgttagaaaa taaactccgg aacccagatc
     8101 aggaacctca attttttctg tcattgagtg ctgaaaaata tgcgggagtg aataaactaa
     8161 tttgccattt atgtcaaaaa tccggagggc tgaagaagat gcatccaagt tttcctgttc
     8221 aattgtaaac actccgttgt tgggattcgg atatatcttt acttctttta ttatgctgca
     8281 gttttgatca attcctgcat atccgttgga attggtttta atcaaaaata tttccgatcc
     8341 gtttccgtag ctacttgatg aacccccgat gaggagcccg ccgtctgctg tttgcataat
     8401 ggatcctcca ttatcgtttt caatgcctcc gtaagtttta gtccataggg tgtctccatg
     8461 tagatttgct tttgaaacat atacatcact tcctccggaa ccaaaactga aagaggaaca
     8521 agacaataca atgccagagt catttgtgac gattaatctc cctcccaaat catcctccgt
     8581 gccaccatag gttttaaacc agatttcgtc tcctatgctg ttatatcttg ccaaaaacgt
     8641 gtcgtaacct ccatatccca cgctgtttgt tctacctgac aatacaaaat cgccaccgct
     8701 caattcaaca acactggaaa cccagtcatt tccccctttg tcatataatt ttgtccacaa
     8761 ggtgtcgcca ttcatatttg tttttatcag catgtaatca tgcgttaaga gtgtattaga
     8821 cagcgttacg ccaatcagca agccggtatc gcttgtttca attacatcat aaccagtatt
     8881 aacatcttga ttgtaaaaag tttttgtcca caaggtatca ccagtttcat cggttttaat
     8941 taaatatata ctggctgtaa ccgggctgaa acttcctgta tttcctgcaa caactaaacc
     9001 attgtcgaag gtttgaataa tgctataacc tgcatctgat ccagttccgc catacgtttt
     9061 tgtccataag gtatcaccgt tttgatcgat tttaattaaa tacacatcgt gattgccata
     9121 tccgtagctg tcagttattc cacatatcgc ataattcccg tcataagtta caattatatc
     9181 acatgacaca tcataacctg atccgccata cgtttttgtc caaatagtat ctccgtattc
     9241 atcggttttt atcacgtaaa tatcccaatc accaagtcca tagctatcgg ttgaaccggt
     9301 gactatatac cctccgtcgg aagtttgaat acaggtgctt ccataatcat caccacttcc
     9361 accataatgt cgttggaaca taatctgggc gctggtctga gctggaatga gagcgagaat
     9421 gagcgtaaac gtgatcgctg atatttttaa taatttcatc agaatttgtt ttgttataag
     9481 tgtggtgaat tttaattcgt atcatcctgc gtgcagaaat attcaatggt tttttcggcg
     9541 caggtgcggc aatagcccag cacgttgatc atggtatcga tcatgcggtt gtagagccgg
     9601 ttgttctctt cgttgttgcg gttcgacagc gctcccacca ggcttccagc gccggcaatg
     9661 tcgctcttga ggcgaacatc ggtcacagct ttcaccagat cgttgttgtc catgaaattg
     9721 taattcggat cggtgttcat cttctgtccg tagatcttgg tgatggtcgt tctgaagctc
     9781 tgtttctgtt cttcgctttt gagtccgagc cgctcttcca cactgttgat gtatgattcg
     9841 tcaatcttga tgctgatgag ttttttcgac tggggatcct gataggtcca gattttattg
     9901 gggccgagat tcttcgcatc gatgccgatg atcatgttca cataattcat cacgtctttt
     9961 tcaatggcat tggcctcatt catgtaggca ttaaagatgg cggtcatcac gttttcgcgg
    10021 tgcagcttcc gtgcgatttt cacatcgttc atgaatttga tgcggtcgtt ctgatcctgc
    10081 acgtagtcaa taattacctt gtcaatggcc gagaaaacgt cgtaggcata catgcactcg
    10141 ccttcctgtg tttcgctctg ttcgagcaga atctgaatgg cgcgtccgag attccgatgt
    10201 ccgagtcctt tctgcccaaa gcggcgtgtg atgtcgggat ctgaattcag ctcattcacc
    10261 acttcggcca gtgtgcgcat gctcttctcg ccagccacct cgccggcagc gagtttcatc
    10321 atttcgaccg gattcagttt ttcattgccc ggcatgcggg tgagaatgaa tgccgttgag
    10381 atggcctgat tgagattggg gtcgatgtgc agttttttcc cgacaaagtt ggttttctcg
    10441 atgctgccca gcgaatacga tgtcaacaac ttttgcagat gcagattggt gttgtgcgac
    10501 atgtaagtca gacggcagcg gtcgatgatg ggtgcctgtt ccttttcctc ctggaagcgg
    10561 gcaaattcgg cgttgttcga agtagctaca attaaggtgt cgagcggcca tttgaatcct
    10621 tcaatttcga tggagcggtt ttgaatcacg cccaaataaa cctgaacgag atcacgtttg
    10681 tttttgaaaa cttcgtcggc gaagtgtatt ccgccaccag ccacacgggc cagagcgcca
    10741 cggcgcaaat cgaagcggta aggattgtcg gcatcggcca gatgcagcag gcggctgatg
    10801 ctttcgtcgc ccagcaggtc aaccgagctg gatgtgattt tatccttggc ggcatatttt
    10861 ccggtcagag ttccgcgcga ttcgctcacc ggcacaggaa caatttcaat atgtttcaga
    10921 atctcgccca gatcgccccc aaaatgctct ttcatttgat tgtaaacaaa ataggtacat
    10981 gctcccaacg gccggtgcat tttgaaaagc ttgtcgagct gcgcatcggt atatcccagt
    11041 tttgacaggt actttttgtt atcgtcgagc gactcaaaga ggttcatagc cagcaccatg
    11101 gggtcttcat aagtctggct ttcgatacgg tcgattttcc cgtaacggcc agtcagatcg
    11161 acattgttga aacggaaagt ataacgttga ttttgaggta ttgaaataaa ttcgcggtat
    11221 ttagtgctga ggaaatcaac aaagaacgtt ttcccattgc ctggttcacc gatgagcaca
    11281 aaagccattt cggcagaact gccaccttcg gcagcatctt tgacaaacga aacgaagctg
    11341 tttatttcgt catacatgcc cacaatatgc tttttacctt cgcggaaaag tttgaaatcg
    11401 taagtcggac ggccgtttat ggtcaccttt tccactttat cgggtccatc gagaatcatt
    11461 cgcgaaaccg attgaaagac attctcgaat ttccgttcac cggagataac tttcagaata
    11521 tgagcatcga gcgatgccgg attcaaagcc gacaaatctg tgctggtctt ttttgccatg
    11581 atgttgtttt ttatcgttta atttcgacca caagatagga gaattttcag tattatcatc
    11641 ataaatatag taaaaattaa tgaagtgaaa aaacattgca gctaatgaaa aaatcaatta
    11701 gatcgaatat tcaggcattt atgtatgtat ttattgtttt tttattcgac tttttttcgg
    11761 tttgaatcaa ttatttcggg ttatcaatcg gttaacaata taaaagtctc aatacaagta
    11821 tcaaattgat aatcagaata cttagcaaca gaaaacaaga agatgagcaa caaacaaata
    11881 aaaaaatcag aattcacata taaatgtgat tgcaaataca tataaatgtt atatttgtcg
    11941 gcacgaaatt gaattcccca aattgaaaaa gtcgtttatc atattattgt ttgcattgct
    12001 ttatatttca ggcagttcac aaacgaaaat gcctgaaann nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnct ggcagacatg gaaggagtag catcgtgtta tctgagtctt
    12181 tcattcatca ataatcgcat tgccgattat cccaaagcat tggaacaggc ctccaattcg
    12241 ctcatattat acagaaaagc aggatcgctt gaagggatgg cactggccta caacaatctt
    12301 ggaaacatct atgataatat gagcaactac aaacaatcgc tccgttatta tcataaggcc
    12361 cttgaaattc atagttctgc gaacaataaa atcggattag cccgcgtata tatcaatctt
    12421 tcaatcattt acaacaaaac aaacaagccc ggggaggcag aagagctatt gaacaaagcc
    12481 attgaaattg gtgaagagac aaaagacaaa agacttttgg ctatagcata cggcaacatg
    12541 gggttgatgc gtgaaaacgc agggaattat cccgatgcgc tcgaatatta caagctctcg
    12601 cttgaattga aaaccgagct tgaagaccgg cacagcatca tgatttcaga aataaacata
    12661 ggcaatactt atctgaaaat gaatcagacg gatcaggcct tgcaacacat caacaaagcc
    12721 gttgaaattg gcaatctact caatgccaaa cgcgagctta ttgacgccta tagcattcaa
    12781 gctcaattat atgaaaggcg tggtaatttt tccaatgcat atgcagccct taaaatgaaa
    12841 actgcgctga acgattctat tttcgggacc gcccagctaa aacacatggc cgatgttgac
    12901 cgaaaaaatg aaatcaatca gctcgaaagt gaaaatcaga ttctgagaca gaaaagcatc
    12961 attgatcagc tgaaactcga gaacaatcgt tttatacgtt attatctgct aatcggactt
    13021 tcatttgcca ttgtttttat cgggttgcta atttatcaat caggtgaacg ccgcaaaagt
    13081 gctcgcagac ttgatgaagt caacaaacaa ttaatcaaga caaacaagca gcttaaacga
    13141 agtgaatcgg cactggccga atcgaatcat gcaaaagaca aattcttttc tatcatttca
    13201 catgatttaa gaaatccact ggccagcatg gtgagttttg taagaatcat gaaacgtgat
    13261 tatgccacat tggaacaaca tgaacgcgat gcgctgattg aagaatttga gaaaatcgta
    13321 aaccgcaccg gcaacctgct tgaaaacctt ctgatgtggt cgcgttctca aacagggcga
    13381 ctcaacatca ctccggaaaa gtttaaagcc cgtaacatcc tcgatgaaaa tgcagaactt
    13441 catgaaacct tgctgcgaac aaaaaagata agaatcagta tagaagttga aaatgataat
    13501 acggaggttt ttgccgacaa aaacatgatt agcacagtcg tcagaaatct gttatccaat
    13561 tcaatcaaat ttacgcccgc tggaggtaaa atagaactgg ggttcaaaca ttccggcaaa
    13621 aactgtgttt ttcacatcaa agacagcggc attggtattg atgccgacaa aatacccagc
    13681 ttattctcgc ttggagaagt ttacgtacgt acaggtactg ccaacgaaaa aggcagcgga
    13741 ctaggactgg tgctttgcaa ggagtttgtt gaaaaaaacc acggtgaaat atggctcgag
    13801 agtaaaaccg gcgaaggaac aactttttac ttttcagttc cgcttttttc ctgatctgct
    13861 gcaaaacttc cctgaaaaaa ataaagggaa taggccccta taaaagcaat actgaaaatc
    13921 atatccggaa ggtcagtaaa aaaaacgact actggcatca gattccacat cagatatggc
    13981 actatcaaca acaccagctg agctacagag tacataaaat atccagcttt gcgcaatcgc
    14041 agcatctgta atgcaccaac aaaagacaac ccgtaaagca ccacctttac ataaggataa
    14101 agaaaatgtg catcataggt gagtgaatag atggaagtat agccgggaat acgggcaaaa
    14161 atcctgatca gctcaatatc aataatactt aacacgccta tcagaacgcc caaaagacca
    14221 ccacccatcg acaaaatgca taatccggtc aatacaaccg atcttttacc tttttccata
    14281 ttccgaaatt tgaggaggtc aaatttatta cttctttttg aaatggatgc cgcaaaatta
    14341 attcaggcaa aaccaacgga ttatgatata aagcatattc gcttgcaatc agacctttgg
    14401 aaagatatat ttatccatca aacaataaca gcatgaaaaa actattttta acaacacctc
    14461 ttttcaacaa cgatattacc accttgttcc cgcgaatcat actcggtctc gtgtttttgt
    14521 ttcacggcgg gcagaaaatg tttggatggt ttggtggcgc aggtctcgat gcaactgccc
    14581 aatttatgaa tatcaatctc gatattccgg tttttatggg ttatatggcc gctatgaccg
    14641 aatttttcgg agccattttt ctgttgcttg gactattcac cagaatcagc gcttttggat
    14701 taggaatcac catgtttgta gccattatca ctgcacatcc cgatgctttt ctgctatcgg
    14761 aaggcggcat ggaatattcg ctcacattgc tttttatgtc ggtgctggca ttcattctcg
    14821 gtcccggcaa aatcagcatg gatgcggttc tgtttaaaaa atgcatctca aaaaaagcat
    14881 gccgggagta gcttctgaaa attggatctg agttggtttt tccgacattt taccgtcagc
    14941 aaaaacaggt tgacatcaca gcattgctga cgtggaatcg tcgggaataa ttgagcagct
    15001 acaaaaccaa cgattccgtg tctgctcctc tgcttaaaaa aaagtccggc ggacgacaga
    15061 atgttggttt cccgacattt taccgtcagt ttgtatggat tgattactta gcattacgga
    15121 tgtagaatcg acagtaaatg gcaaaaacaa gcaatgccag cattattatg cttttttcta
    15181 ttgtggaaaa aagaaaatgt ctttttttat tccctgtttg gagattttta aataatctgt
    15241 atctttgcac tcccaaaatg gtgaaattag ctcagtcggt tagagcatca gattgtggtt
    15301 ctgagggtca tgggttcgaa tcccatattt caccctgaaa atccgcccca aaggcggatt
    15361 tttttattca gccagatcgt tattattcat gttttctctg cggtttttca tttgtttttt
    15421 ctgactggca agaaactcct gaaaatctct ttctttaatt ttttcaagcg ttcgaagcca
    15481 tttacgtctt ttcatgaacg gagaacggct ctgtgcttta ttgatgaaca acaccgcaat
    15541 gacaatacct gcaaacaaaa aaccatagga tacaaactga aagtcagccg gcatccacca
    15601 tgccagcaaa aaaaggtcgt aaaacccatg ggcaaaaaca gccagcccaa ggcctgtcaa
    15661 aatcaaccag aatcggtgtt taaaataagt gaaccgggcc tgtcccataa aataccccat
    15721 aaaaacagca aacataaaat gcgccggcac cgccgtgaat gaacgaaaga tggctgttac
    15781 ataaatattg tgagcaccaa caagatgcaa aaccgattcg gcaaaagcaa aaccaagcga
    15841 aagcatcacg gcatagacaa ttccatttat cggctctgaa aaaaaacgct tgcggtaaaa
    15901 aacgatacgc aaaacagcaa atttgcacaa ttcttcggtc aatgcaacaa caacaaaagc
    15961 attaagcatc agagatccga agtttggact gatgcccatt cccatgcgcc cttcgagcaa
    16021 acgttcaatc actatggccg gtgcaatagt cagaatacca gccacaaaag ccagtaaaag
    16081 ccaaagcatt ggttctcggt cgtatttatc tttgagataa atgaacaaac caagagcgag
    16141 aacgggtgca aatgctaaag aggcaataag catacaacgg aatttcgtaa ctttacgcaa
    16201 aaatagcccc aaaattatta tgaaaaccat tttcagcgaa aaaatcaaca ttcaggaagc
    16261 caacaaacgc ggaatcacat cctggcccat ctgggaaaaa gaagcttcgc gcttccctgc
    16321 ctcatacgat gcaaaagaag aatgctacat ccttgaaggc gaattttgca ttgaagctgg
    16381 taatgcgtta ttcaattttg gcccaggcga ttttgtggtt ttcccggcag attttgaatg
    16441 tatctgggat ataaaaaaga aagtaaaaaa acactataat tttcctctgt aatgaaaact
    16501 tttcacgctg gcgacaaagt tcgctttatc tcatccaaag gcagtggcat tgttacttct
    16561 gtcaaaaaaa acattgtgca tgtcctcata gaagacggat ttgaaatacc cgttgaagca
    16621 tcggatctgg tgattattga tcagcaggaa ggcaaaaaaa atccttttgc aaaaaaagat
    16681 gaattcagca aagttcaatc caatacagtt atatcgaaaa ataaaagctt tatatctaaa
    16741 aaaagtgata attcgactac aacatcaagc aattcaccaa ataaggaaaa tcaaaaaacg
    16801 caaaacacct cagtcagtcc ggccacagga atttatcttg cttttattcc tgataatcaa
    16861 gatgttctta tttcagggaa catggatatt tacctcatca attacagcaa atggcaagta
    16921 gcttttcacg cattgaaatc cgacccgggc aaaggatttg ccagtgtttc atgcggattg
    16981 atggacaagg gcgacgcgat actcattgaa acggtaacgg agcaagatgc ccgccattgg
    17041 aaccggctcc gtcttcagct tgtttgtttc gaccagaaag aaagccagtt gtttgattct
    17101 gaaattgttg atatcgatat aaaaacttcg aaattcatca aggaagatat ctataccgaa
    17161 aacccgtttt tcgatgaatt ggctcacgta atttttgtgc acgacctgac gcatcctccg
    17221 ctgaaaggtc gaaaatcgga tatgattcac gaaaatccgg aaaaaaagac caacacggtc
    17281 atcgaccgtt atatgaccga taaaaacacg gctgaagtgg acttgcacat agaaaaactg
    17341 cggagtgacc ataaaaccat gcgcaaagac gaaataaagc cggttcagct ggcttttttt
    17401 cgccaatgta tcgatgcggc cattacaaaa aaacttagtc gtgccgtatt tatacacgga
    17461 gttggagccg gattgcttaa aaaagagctg actgatattc tgaaaaccta tcccgatctg
    17521 gaatatgagg atgccagcat tatgaaatac ggtattggtg caacagaggt acgtttcaaa
    17581 aatcgataac ccatttcatc atagctgaag ccacattccc aagcatcaac tgatactctt
    17641 tgcggaaaaa tcgcgagtta tataatgtct atttcatatt ttgtgcgtaa tttcggctcc
    17701 tgaaatttag tatggtatgt ggaacagaat tgtcaattac attctcagaa gaagagcgct
    17761 caatcttatc gttatcgggt tactgacagt ttttatgggc tatcaggccc tcaaagtaaa
    17821 gatgtcttat gaatatgctc agatgcttcc gatgtcggac agcaccagta taatatatca
    17881 ggatttcaaa aaacaattcg aagaagatgg cagtgttttg tttgtcggca ttcaggatga
    17941 aaaactcaaa aaactggaag tcttcaacga ctggtgcaat ctttcacatg atctgaaaaa
    18001 cataaaaggt gtcggcgaag taatgtcaat tgctcattgt ttggacctgc aaaaagatac
    18061 tgctgcaaaa aaattcaaaa ttgtcaagct gttcgaaaca aatccttcca gtcaggccga
    18121 acttgacagc atcctgaaaa aggcctattc aatgcctttt tacgatgaaa tgcttttcag
    18181 caaagaaaat ggcacaaacg taatgatggt gtatatcgac aaaaaagtcc tgaacagtaa
    18241 agaacgggat aaagttttga ttgatataaa agaacccatc gaagaatttg gagtgaaaca
    18301 tgaaatagaa atgcattttt cgggcatgcc ctatatcaga acatccattt caaatctggt
    18361 tcagaaagaa cttaaagttt ttatgattct ggctctgatt gtggcttcca ttattctgct
    18421 ggtatttttc agatcgctca aggcattggt tttttcaatc actacagtca ttattggtgt
    18481 tatctgggct gttggaacaa tggttctcat gggcttcgac atcactattt tgtctggtat
    18541 cattccaccc attctcatta ttattggtat tgaaaactgt atctacctga ttacaaaata
    18601 tcattatgaa tttgcccaac acggcaatca ggccaaatcg ctggcacgtg tggtacaacg
    18661 cgtcgggctg gccacatttc tcaccaatgc cacaacagct gtgggctttg ggtctttcat
    18721 ggttacttca aatccaatca tggtcgaatt cggaatcatt gcctgcgtga gtattctgct
    18781 cgaattcctg tttacactca ttctgatacc caccatgtac agtctttttc caccacccaa
    18841 aggccgtcat atcaaacatc tcgatttcac ctggctcaat ggtgccacac gttttatcat
    18901 caatactatt caatacaaac gcaatttagt ccttgtagca gtcatcatac tgctcaccgg
    18961 agcaggtttc ggaatttcac tcattgaaaa caaaggcagt gttgtcgatg atgttccaaa
    19021 aggtgacaaa ctttacaccg acatgttgtt tttcgaagac aacatgggtg gagtactacc
    19081 acttgaaata accgttgaca ccaaaaagaa aaaaggcatt ctgaatctga atacattgaa
    19141 aaaaatcgac aaatttcaga cagaactgaa agaaattccg gccatttcaa aaccgctttc
    19201 agttgtcgaa attgtaaaat ttgccaaaca atcgtttttc aacggaagac ccgatatgta
    19261 cagtctgccc aatgacaatg agaaagcatt tattctgagc tatctgcccg atattgacaa
    19321 aggcggagaa aagaacagta ttctttcgag ttttattgat acaaactatc aggttgcgcg
    19381 catcagtatc cggctaaaaa acgtttacac acctgaaatc aaagaaataa aagacacgct
    19441 cgaaagcaaa ctggccagaa tttttccggc agaaaaatac aaaaccgttg ttacaggttc
    19501 tgccgtggta ttcgaaaaag gcagcaatta tctggtttcg aatctctatg gaagtcttgc
    19561 actggctatt gttatcattg cgctgttgat ttacatattg ttcagcaatg tcaaaatggt
    19621 atttattgcc atgaccacca atatgattcc attgcttgta acagcaggcc tcatgggctt
    19681 tctgaatgtc gatctgaaac catccaccat tataatttac agtattgcgc tggggatttc
    19741 ggtcgatgca gctattcaat tcctctcgcg ttaccggcat caactgaaaa tgacagactg
    19801 gaacatcaga gaaagtgtat tgggagcgct gaaagaaacc accagcggaa tgatattctc
    19861 aggcattgtt ctggttcttg gttttggcgt atttattttt tcacgtttcg gaggcacagc
    19921 cagtctggga tatctgatcg gatttacttt gctgatagca ttgttcagca atctgattat
    19981 tctgccttcg ctgattctgt acatggacaa actcattaaa aacaaagaag ccagcaaaac
    20041 actgtttgat gttgaagaaa acgacgatcc ggtgctgcct ccgctcaatg aagaacaaca
    20101 ataaaaaata aatcaatgaa aggtattatt cttgccggcg gctccggcac acggctttat
    20161 ccaattacca tggcaatgag caagcaattg atgcccattt atgacaaacc catgatttat
    20221 taccccctgt cggtgcttat gatggccgga atacgtgata ttcttattat ttctacacca
    20281 cacgacctgc cgcatttcaa acatctgcta ggcgatggaa gccagatcgg atgcaatttc
    20341 agctatatcg aacaacaagt tccaaacggt cttgcacaag catttgtgct tggcgaacaa
    20401 tttataggtt ccaacaatgt ttcattgatt ttaggcgata atatttttta ctcttctggt
    20461 ttacacgaaa agctgcatgg catgaaaaag cccgacggag gtattgtttt tgcctatcat
    20521 gtaagcgatc cggagcgata tggggtggtt gaattcgacg aaaacatgaa agccatcagc
    20581 attgaagaaa aaccatcaaa accaaaatcg aattatgccg ttcccggttt gtatttctac
    20641 gacaacagcg tggtcgaagt agccaaaaat ctggcaccca gcgcacgggg cgaatacgaa
    20701 attaccgatg taaacaagca ttacctgcat gcccgcaaac tcagcgttga agtcctgagc
    20761 cgcggaaccg cctggctcga caccggaaca ttttcgtcgc tgctgcaggc aagtcagttt
    20821 gtcgaagtca tcgaaagccg gcaaggactt aaaattgggt gcatcgaaga aattgccttt
    20881 acagaaggct ttatcgataa aaaacaactc gaaataattg cacagccatt actgaaaagc
    20941 ggttatggtc aatatctatt atccttattg caaaaataat gatctctgtt ttactcaccg
    21001 gcggagccgg attcataggc agcagtcttg ctgaaaaact actcgaaaac ccggatattt
    21061 ttctggttat tgtagatgat ctttcaacag gttccattga aaaattacct attaaacaca
    21121 ctgaaagata caaatttatc aaggccgatg taaatgccta ccgcgacatc tccgaagttt
    21181 ttcatgcatt ccgcttcgac tatgtttttc actactcagc catggttggc gttaaacgga
    21241 cactcgaaaa tccggtcagc gttttgctgg atctgaaaag cattgaaaac attctcaatc
    21301 tgagcaagaa ttcaacggtc aaacgtgtgt tttttagctc cagctccgaa gtgtacggcg
    21361 agccggtcga gtttccacaa aacgaaaata caacgccact gaattcaaaa ctgccctatg
    21421 cgattgtaaa aaatgcatct gaagcttttc tgaaatccta ccataccgaa ttcgggctcc
    21481 cctacaccat tttcagattt tttaacacct atggcccgaa gcaaagcaaa gattttgtca
    21541 tcagccgctt catcaatctt gctttgaaaa acaaaccact gaccatctac ggcgacggca
    21601 aacaaacccg cactttttgc tacatcgacg ataatgtgga tgcaaccctg aactgcttta
    21661 atcaggttat tcaaaatccg gccatgcaca gtgatgtgta caatatcggc aatgacaatg
    21721 aaacatacat cgatgaagtg gccaaaatga ttgtcagaat aaccggttca tcatctgaat
    21781 tgaaatactt gccgccactc aaggaaggcg atatgcgccg ccgcctgccc gatatcggca
    21841 aaatgcacaa actgctcaac cggccattgc ttccactcga agaaggcatt aaaaatattc
    21901 tgaacgatac ccggtttata atctgacaaa agcatgaaaa acaccataca gcattatcag
    21961 gaaattttca ctgaacgatt caataaaatc aattttgtaa aattccctca tgagctgtat
    22021 gagcctatcg attatgcact gagggcgggt ggaaagcgtt tgcggcctgt tctcacaatc
    22081 atgatatgtg atttctatgg caacaacata gaggacgcca tgaatccggc catcgccatt
    22141 gaaacctttc ataattttac tctggtgcat gatgatatca tggacaatgc acccattcgt
    22201 cgcggaagac caaccgttta caataaatac ggaaccaaca tcggaattct ttctggcgac
    22261 accatgttta ccaaggcata cgaatttgta acccttacgc gcgaggacaa acttccggcc
    22321 gtattgaaag tattcaccaa aaccgccatg gaagtttgtg aagggcaaca actcgacatg
    22381 aatttcgaga ccatgcctga agttaccatg gaagaataca tgcagatgat tcttctgaaa
    22441 acggcagtac tgatgggtgc ctgcctgaaa ataggggcca ttctcggcaa cgcaacggcc
    22501 gaacaaagcg aagccattta tcgctttggc gaacacaccg ggatggtttt tcagctccag
    22561 gacgatctcc tcgacacttt cggcgacgaa aaaacctttg gaaaacgcat cggaggcgat
    22621 attctggatt gtaaaaaaac ctatctgtat gtcaatgcca tgggcaaatt aaaaggcgac
    22681 gagcgtcagg atttccagca gctctacaat aataaaacaa tggatgaagt cgaaaaaatc
    22741 atccgggtga aagaattttt gataaataac aatgtcattg ctgattgcaa agcagtcatg
    22801 cagaatcatc acaatctggc catggaagcc ctttctgaaa caggattgaa agcagatcag
    22861 aaagctatcc ttcaggagtt tgccgaatcc atgctggtaa gggatttctg atagccagag
    22921 tcaacgcatt aaaatggaaa aaaatcttct gtatcgtata taaacagagc tccccaaagt
    22981 tttacccagc gtaatattta gtccgcttca gcgaatcgga gcaaaaaaag catgacattg
    23041 cacgcttgct gtcagtattg aatgcattaa tcagagctat tcaaagtgat ttaaatgcgt
    23101 tgaccctcac ttcaaacttc ggagctcctt gttccataat caatattcgc tacctttgca
    23161 ctccaaattt tatattatgg ttcgcgtacg ttttgcaccc agccccacag gtccgcttca
    23221 tatcggcgga gtgagaacag cattgtataa ttgctttttt gccaaaaaga atggcggaaa
    23281 actcattctt cgcatcgaag ataccgatca gacgcgtttt gtggaaggtg ctgaaaaata
    23341 tattattgaa agtctcacct ggagcggaat ccatttcgac gaaggtccgc atgtcggcgg
    23401 tccctttggc ccttataaac agagcgaacg caaagccatg tatcatcagt atgccatgca
    23461 actcattgaa agtggcaatg cctattatgc tttcgataca gccgaagaac tcgatcattt
    23521 acgcaaagaa tgcgaatcga aaaaagaaac tttccaatac gactgcctat caagaaaaaa
    23581 cctgcgcaac tcgctcacca tgccggaaag cgaagtaaaa aatctgctcg acagtggcac
    23641 aacatttacg attcggctca aggtgcctga aaacagtgtc atcgagtttg acgatctcat
    23701 tcgcggtcat ataagcgtaa aaagcgaaaa catagacgac aaggtgctgt tcaaaagcga
    23761 cggcatgccc acctatcatc tggccaatat tgttgacgac catctgatgg aaatcaccca
    23821 tgtaatacgg ggcgaagaat ggcttccatc ggctccactg cacgttttgc tgtatcgttt
    23881 tctgggctgg gaacgaccac agtttgcaca tctgccattg ctgctgaaac ccgatggcaa
    23941 cgggaaactg agcaagcgcg acggcgatcg tttgggtttc cctgtatttc cgatgaactg
    24001 gaccgatccg aaatcgggag aacaaagctc cggataccgc gaaagcggat attttcccga
    24061 agcattcatc aatatgctgg ctctgcttgg ctggaatccc ggcgacgaac gcgaaattat
    24121 ggggatcgaa gaaatgagtc aggccttttc gcttgaccat gtaagcaaaa gcggagcaaa
    24181 attcgacccc gtaaaagctc ggtggttcaa tcaccagtat ctgatgaaag ccagtattga
    24241 agacatttcc gaagaatttg aatcattgct gaaacaacaa aacatatcca ttcctgataa
    24301 gcaaaaacta aacacgataa ccgggctttt acgtgacaga gttgattttg tgaaagattt
    24361 gcccgaagca ggtcgctttt tcttcgaggc gccaaaactc tttaacgaag cggatctgaa
    24421 aaagcacagc acgcctgatt caggcaattg gctcaatgcg ttgaaagttg tatttgaaaa
    24481 tacagactgg tctgcagcag ctattgatac agccattgct acatttatca aagagggttc
    24541 actgcctgct gggaaaatat acaatatcat tcgcattgca atggtaggag gcagttccgg
    24601 gcctcatctg gcagatattt tacttctgct gggcaaagaa gaaacactga aaagagttga
    24661 tgcgatgatt caatttctga caaaacactg attcccaggt tatgaaaata cgcaacaagc
    24721 gtaaagagaa acatatccat aaaatcacta attttgccaa aacaacctgc catgccaatt
    24781 ataggtacgt taataaaaag tgcgattgag ctgaaaaaca tgatgcctgt agaaaagcaa
    24841 aagccatcag ccatcacaca acaaaaaaaa gtacttaaaa aactaattcg caaggctcaa
    24901 ctgactgcat ttggagaaca ctacgatttt gccggggccc tgaagcagga tgatttactc
    24961 agtgcattca agcaatctgt tccgattcat gattacaata agatgcacaa cgactggtgg
    25021 tatcgcactt taacgggcga ggcctacgta agctggccag gcagggtaaa atattttgca
    25081 ttaagctcag gcacaagcga agccaccagt aaatatattc ctgtaacgcc ggaaatgctg
    25141 cgcgccataa aacgaacaag catccgcaca ttggtagcat ctgcccgata cgatattccc
    25201 accgattttt tcaaacgcgg catactcatg ctgggtggaa gcactcatct gcaattcaat
    25261 ggaacttatt atgaaggaga cttatccgga attactaccg gcaacatccc tttctggttt
    25321 cagcatttct ataagcccgg aaaacgcatc agccgcgagc gcaactggga gaccaaaatt
    25381 gaagaaattg tacgtaaagc ccccgaatgg gacataggag ccattgttgg cgtcccggcc
    25441 tggtttcaga ttctgctcac caaaatcatt gagcgctaca atctgaataa cattcacgaa
    25501 atgtggccca acctgtcggt cttcactcac ggcggggtta gttttgcccc ttatgcaaag
    25561 agctttgaaa agctattagg caaaccaatg atttacattg aaacctatct ggcttctgaa
    25621 ggatatattg cctaccagga tgatccggaa agcgattcca tggcgctggt acttgacaat
    25681 ggtctgtttt tcgaatttgt tcctttcaat gataaaaact tcgataatga aggaaatatt
    25741 caaaccaatg caaccgccta taccatcgat gaagttgaag aaggacggga atatgcttta
    25801 ctgataagtt ccgttgctgg tgcctggcgc tatctcattg gcgatgtggt caaattcacc
    25861 aatctttcaa aggcccagat tcacattgtg ggtcggacaa aacactatct gagtgtttgt
    25921 ggcgaacatc tgtcgcagga aaatatgaac cgtgccatag aaatgcttga acatgattac
    25981 aatatttcag tccctgaatt tactgttgcc gggcttccgc acgacaatat gttccaacac
    26041 aaatggtggt taggaaccaa tgatagtata gatccggaaa tcgcagccaa agccattgat
    26101 aacaatctaa aacaactcaa cgacgattac cgggtggaac gaattgccgc catccgaaaa
    26161 gttgaagttg aagtattacc tttgaatgta ttttacgatt acatggcttc aaagggcaaa
    26221 atcggggctc aggtaaagtt tccgcgtgtc atgaaaaaac atctacccga ctggcttgaa
    26281 ttccttaaca acttgaaaaa ataagaatgg tggaaatgct gggaatattc atcgaaggga
    26341 ttttgcttgg gctggttttg agcattaccc ttggacccgc tttttttacc attatccaaa
    26401 cttccatcga ccggggattc aaatcagctt ttcttattgc aattggtgtt gcaaccagcg
    26461 atttatttat cattacaatt tcatatctgg gcctatcggc tgttatagaa tccggcaaaa
    26521 atcagattta tgcaggcatt gttggcggag ttattctggt aatgtatggc gtgtacacct
    26581 tttttaaaaa accggaaata ttgaagcgcc gatcatcgcg caatactgga gtagcgcgcg
    26641 aaaccaaacc cattaccttt ttggccaaag gattctttct caatattgcc aacccattca
    26701 tcatactttt ctggctcact attattggct atatttctca aattgccgaa tacggaaagc
    26761 tcgaagaaac ggctactgtt tttctttcag gcaccattct tacagttttt tcaatggatg
    26821 tactgaaaag cttcattggt tacaaaatca aaaaatatct gcgtccgaga atccaactgc
    26881 tgattaatcg gattgttggc ttttcacttg tggtttttgg aatcattctt atcattcgct
    26941 tgcttgtata aagaccttgc aatgaaaaag agaatttctt atctttgtac aaattcaggc
    27001 cctatgaaac cagccaacaa atatttccgg gttttacttg ttgccttttc tgctgctgct
    27061 ttgtttttaa catccaattc gtgtaaaaac aggcaacaac aatcaaaata cggtccccct
    27121 tctgacacct atcaggatca accggtaaca aaatatggtg taataactga tactattgga
    27181 ccggttcaga caaaatatgg ggtcccggta cctgaaaacg agtaaattat ttccggccaa
    27241 aatcggctgg aatttcaccc cagacatcgg tttgccattt ccgcacttca ttgtgtaccg
    27301 gattttcttt caaccactct tcggcacgtt gcaaaagctg aaaaacccga acattttcag
    27361 atgttctttc aagtttggtt ttggcttttt tgtttctcaa ccatgccatg gccgttgccg
    27421 aatcagaata taaaggaaaa ggcatgttgt tctttttcaa ccatgaaaga ccatgtacca
    27481 gagcaataaa ctctccaata ttgttggtgg catctttaaa tggcccttcg cggaaaatca
    27541 gctgcccatc ctgacaccag actccctgat actccattac gcccggatta ccactgcatg
    27601 ctgcatcaac acagatgcag acttcctgtg gtctgagtga gtgatcaatc tgttttcgct
    27661 tcactgactt cacacctgct tcaaaaccgc acaaataagc ctgttcagct tcttcgcgtg
    27721 taacaaaacc cttgtatttg gctcccggga aacccgaaat ttgttcataa catttatccc
    27781 aatccggata aatgccagga aaacggcctt cccaaacaac ataaaaatta ttctttttct
    27841 tcgacatccg gatgttgatt ttgtccggca gcagatctgc ggcttccctg ataggcttcg
    27901 aaacaaagaa gccggctgtc aatcattcca ttcagcaatg gaattttcaa ggttgccttc
    27961 aaaccaatgc ttttccataa atctatattg ccaaccagca tccagacttt agaaccctgc
    28021 agctgatttt tgatggtatc tccaattttt ttataataat cacgactgtt ttctatggga
    28081 agtctggcgt cataaggcgg attgaaaaca atgtgcgcag gactatcggg tgcaatatcg
    28141 aaaaagttca ttcggcgaag ctctatatcc ctgtccagac gagcttttcg aatatttgta
    28201 tgtgccgctt tcaaagaatc atcggaacgg tcagagccaa taatattgaa ttcaaattct
    28261 ttggtctgcg caatcgcttc ttttttaacc cgttcaaaca tagccggttt aaaagtctgc
    28321 caatgcataa acccaaaatc atttcgataa tatcctgcgg ggatgttatt ggccatcatg
    28381 gcggcttcaa tgagtattgt acctgaaccg cacatgggat cgataaaatc acaatccatt
    28441 ttccaacccg acagccctat aattccggcg gccaaaacct cattcaaagg ggcaacgtga
    28501 gaagcaatac gatagccccg tttatgaagg gatttgccgg aactgtcaac tgaaacaaca
    28561 cattcatcat ggttgatgta aatattaatg agcaggtccg gattgtcttt atcaaccgaa
    28621 ggccgcaatc cgcaacgatt tctgaaaaaa tcaaccactc cgtctttggt aagcaactca
    28681 acaaaatgag tattattaaa aatgggactg gcagttacaa cagcatcgac agcaatcgtt
    28741 ttttcggctc cgacataggt atcccattca aatgcccgga tgtttttcaa aaattcatct
    28801 ttatctgcaa aacgaaagcg caaaacctca accaaaagac gaatgccgaa gcgcgaagtg
    28861 taattcaaac gatacatcat ggcctcgtcg gcttcaaaca taaatcctcg ttttacaggt
    28921 tccgggtttg tgcatccggc atcaagaagt tctttttcca ataatggttc aagacctgca
    28981 agtgttttga atgtgtactt ataaagagta gtcggcatat gaaaacggtc tgaaaagtgt
    29041 atcggtaaaa ttctcacaaa aaaagtctgc aattattcaa aagcaattgc agaccaattt
    29101 taaattgttg aaatcaatta taggcaagtg atgtgaaaac accgtcggta attgatttat
    29161 tcaatgcatt gttttttaca ttgattgcgt tgaagttgaa agttccggaa acacggcgat
    29221 tcgtctgatc aatttcagac aaaacaactg ttccgttgat ggcagtataa agactgtcgg
    29281 tattcgaagc cacaggactg taagaggcag tgaaattgaa ctgcatagta cccagtgtgt
    29341 aagtgcctgt agtttcgcct acaatagtaa tatcaagtgc atcactctgc aacgcactgc
    29401 cattgataga aaaaccaccg gcagtatttg ttgtaacgcg tgtcagagcc gtccactgaa
    29461 caccatcaat tttacaggtc atttttgcac ttggattgat aatagtaggt gtaccatcac
    29521 catcatcagc acaggaagcg gccatcaatc cgatcaaagc caatagaata taaatctttt
    29581 tcatatcatt tgtttttgca aaggtaggtg tttttttgca aaatgctgat ctgaaaaatg
    29641 agggacgggc atcaacacca tccatcttca ccaatctcac ccgccctcat tgactgtctg
    29701 ttggtggcag aatctaacca acgacaccca tgtcctgccc tggaacgcgc tgctcaacaa
    29761 tctgtccgac aatttcagta atagtacgtt tctgtccgtc tttatccacc caattgcgat
    29821 tgttgagttt tccggcaatg tataaacaat gccctttttt cacaatgtct tcgcatgtcg
    29881 aaacgacatc gctccacaat acaatacggt gccagagagt attctgcaca gtgtcaccca
    29941 ttgcgttcat gtagctttcg tttgttgcca gcgaaaaaaa caacattttc tttccggttt
    30001 ttgtttcacg cacatccgga tctttgccta cacggccgat taattcaact ttgttcatga
    30061 caataatttt taaattttac gccacaaagt tgcatcaacc tttaccaatt atccggttat
    30121 taaatactta ttgtcgttta taaacggata taaatactta taaatatttg taatcatttg
    30181 ttgtcgggta ctgaagcatt gaaaacaatg ccagattctg catcctttca gggattggcg
    30241 tttccgcgaa ccactccacc tgtattcact ggcgcagctt tccggaaaat atcatttttg
    30301 ttcagcggcc gcaaacccgc ccgccattca aaacccgcca gttttcgctg atcggaacca
    30361 agatccttat ttgggtagat tgtgccttgc ggagatccaa tcatattaat ggctactact
    30421 tcgctgtttt caaaaaacaa atccatcgac gctgaagagg atttattgat ccctgttttg
    30481 gcaccatttt catcttcaat aaaatatatg gcctcggcat tgcctgtaac cagcatatga
    30541 tcaatattgt tgttttcaaa atacccgttc atctgcactc cctttacctg ctgaaaatcc
    30601 agctcctcgt acattgaaac tataaaagca ttgtctttca tatacaattc ccttatttcg
    30661 ttccggtcaa tggtaaactg aatatattcg gcagtcatct gtgttgaatc tgaccaaaga
    30721 catgggtttt cgaacatccg catcagcgaa tccttttcac aataataaag cgagtcgcac
    30781 actccctgca tgtcgaatcg ataaaaacga acaccataat aggcctgtat ctcacggccg
    30841 agcaaactgt cttttgtagt aaaaagagta tcgccatgca gatacaacga atcagttcct
    30901 tcccaagtga taaagacggc actatctgta gcaagaacag aactgtcctt ttcggaaaaa
    30961 tcacctgaat gtgccagcag cattacattc tcaagtgtat ctctgaatac catattcctt
    31021 cgcgctttgc cgagacccag ttttttatca taatacaaag tatcgccttc aagaaccgat
    31081 gagccattga tgagttttgc acgattaaac aatttcgtct gatcggtcag tgtattatac
    31141 catccatatt cgcagtaaat atccgtactg tcgctctgaa tgtgagtatt accataaaaa
    31201 tcgatctgct cggttatggt gttatacatc aaactatccg aaatcatttt gaaattgttg
    31261 ccagtaacaa ccaccccatg tctgaagaaa aattcctttt tacctgaata ataataaccg
    31321 tgtttactca ccaatgtatt gtgtggatcc tgaatttcgg cactatccag ataataaccg
    31381 attccgtttt caagatcata atacaatttt tgggtatgca acacgatatt gttgtcaatc
    31441 aattccacat cctgctccat aatcgctttt ttctcattgc catcataatg gagcttttcg
    31501 ccataaatct gagttgcatc ggaaacgcgt atgtgcacat tgccgtaggc atccattgcg
    31561 tttttggtga tatacaataa agcactatca caataaagca atgaattctc gtgctgaaag
    31621 accacattgc ctttcagaaa ctgcacatcg ggattgacgg gtctgttata ttcaagaaaa
    31681 tcagccgatt tcaaaagaat cttctttttc tcctgagcaa aagactgcaa agaaaagaaa
    31741 caaaaaaaca aaagcggaac aagcgcagaa acaaacgttc tcaaagatct tcttctttta
    31801 gaaaactccc cttcatatca aacaaaagtc ttttatgctc gcggtatccg tctcttttaa
    31861 taaagacact gtatttcaac acgttttcat cgctgataat aacaccttcg gtaaatgacc
    31921 agccaggata gttttcggca atatacttaa ttgactgaga aggcagctcc ttgtgtgtta
    31981 cactctgacc gtcaagattg ttgccttctt cgagagacaa atcgtcttcg gctgtaactt
    32041 cgttgaaagc tgcatgctgg ttttctcttt tttcgcgaag atacacatct tcagcatcga
    32101 taaaattggc cagcacttct ttttcaagcc ggccgcttgt agaaaaataa atttcgtgaa
    32161 acaccattgg atcattcatc cacgtgggtc catacaattt caccagtgtg tattttttct
    32221 tatcggcata caccagattt tcggcagatt caatttcaag atcaggataa ttatccatca
    32281 aataattctg agccaatggc aaaaggtctt tcggatttaa aaccaccact gacatcacaa
    32341 aagcaccttt tttatcaaaa tgcatcctgt tttcagtact tcgtatggga ttgtaatatg
    32401 aagcaacata gtgcaatcca agtgtatccc aacgaacttc ctctgctttg ggttcttttt
    32461 tggtaaaata ctgtacagcg gcagcaggca ccttactctg aggaaaaggc gttctggtct
    32521 gttttttctg acctttgtcc tgatccttct gatctccgct gtctgaaaca ggtttgttgg
    32581 ctccggctat aggtgtaccc tgaaaatcaa accataattc aacagggacc ttatcgtcgg
    32641 atttgcccaa aacaacataa taggcattct gatatttttc gttgttgaca atatagcttt
    32701 cacgaattga atactggcta taattgatga taacataaga attgatggcc gaaggcaatt
    32761 cttttggatg cacagccagt ttgcctcgtt cacttgctcc ctgcaactgc aggtctatat
    32821 cgttgccttt tgaatcgctt tttctgacaa atgcgcccga aatatcgtac atggcaaaaa
    32881 ggggctgctt tgttagcgta tcaagcattt caacattata ttcggagata tccttgtttt
    32941 cggttaaaag aattctaaca acttttgtat tggcaaagct ggttgtaaca tgtttttgca
    33001 tcgatcgggg acaatcctca atttcatact cggattcgga attaatccat cggcctgatt
    33061 cggcaaattt cactaccact ttgatattgt cataagaaaa agcagcatga taatcagtat
    33121 tatgtctggt ccactgaaca ccagtaaccc ccggaaacag gttaggcaat ttacttttaa
    33181 ctacctccgg aacatcgctt tccttaacag gaagctgggc aaataccatg cattgcatgg
    33241 aaaaaagcat aacgataatc agaaagactt ttttcataac agctacaatt taataatttg
    33301 ttctcggtca ggtccggttg aaatataaga cagatgagct ccggcagatt tttccacaaa
    33361 acgcgagtat tcggtcaatg ccgggggcaa ttgattggcc tgggttgttg tggtgatatc
    33421 tgcactccat ccattcatct ctttgattat cagatcgggc ttacatccgg caggaatatc
    33481 gggcatatcg tacaatttat ctgcgcattt atacgcatca cagactttga tagtttcaag
    33541 gcctgtcaac acatcggctt tagtcattat caggtcagtg acaccattga gcataactga
    33601 atagcgtaaa gccggcatat cgagccatcc cgtccggcga ggtcttccgg ttgttgcgcc
    33661 aaactcattt ccatttttac gaagcaattc acccatgtca tcaaaaagtt ctgtagggaa
    33721 tgggccgtta cccaccctgg tgcaataggc cttgaaaata ccaaaaacct tgcccacatg
    33781 cgaaggagct actccaagcc cggtacaaac gccaccagtc atggtattgg aagaagtaac
    33841 aaacggataa gagccaaaat caacatcgag caaagttccc tgagcccctt cggccaacac
    33901 ttttttgcca gcagccattg atttattaat ctctatttca gtggcagcaa tacggaattg
    33961 tttcatttta tgggcagctt tcagccattc ctgctcgtat tcgtcaaacg gcagtccatc
    34021 gagcaatata ccccgataat cgacacccga agcagcaata agtttcatat gtatctcccg
    34081 gcgcgactca tattcagtct gaaaaccgtt gtgttcgagt tcgcccacac gcaatcccga
    34141 ccgtttgtat ttatcggtat aacagggacc aattcctttg agagtgctgc cgattttttt
    34201 ttcacccata gcggcctcgt ttactgcatc caacaaacga tgcgaaggca taataaaatg
    34261 agcgctacgg ctgatcagca gatttgacga cgggtcaaat ccggcttcac ggatagcatc
    34321 tatttcctga aagaaaataa aaggatccaa caccacccca ttaccaataa tattgagaat
    34381 attgctacga aaaatgccac tgggaacaga atgcaaaaca aatttgcggg aattgaaaat
    34441 aatggtatgt ccggcattgg gaccaccctg aaaccgcgct ataatatcat attgcggagc
    34501 cagtgcatca acaatttttc cctttccctc atcgccccat tgcaggccta aaatcacatc
    34561 aactttcata caatttcaaa caagttacaa aactaacaaa ttaatccttc ttttcaaaat
    34621 ccgcttgccc atctttggaa caatcctcac aaatgccata aaaaaccaaa gaatgactgg
    34681 acgctctgaa accaaaagtc tggagcaggg ttttttcgat ctcccccaca cggggctcac
    34741 aaaattcaat cactttgcgg catttgagac aaattgcatg atcgtggggc tgaaaacata
    34801 aacacttttc gtattgcgcg ctgctttggc caaactggtg cttgaccacc aagccacttt
    34861 caagcaataa ctccatagta ttgtacaaag ttgctctgct aacccgatag cgattgtttt
    34921 tcattcggat atacaacgtt tcgatatcaa aatgacccgg tctggtataa atctctttta
    34981 aaatagaata acgctccggt gtctttcgat gttttgtgcg gttcaggtat tcagtaaata
    35041 tttgttttac agcatcataa ttatcaaccg cctgctgaat ttcggcattc ttttccatgg
    35101 cagaaaattt cagtaaagat aaccattatt tttatttggt ttaaataggg tctgctgaaa
    35161 aacacgtaac actcacaaaa acagaaatat attcagcaa
//
LOCUS       DAOPKE010000022        33874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1361_length_33874_cov_13.847955, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000022 DAOPKE010000000
VERSION     DAOPKE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1361_length_33874_cov_13.847955"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1217)
                     /locus_tag="PKY63_04600"
     CDS             complement(45..1217)
                     /locus_tag="PKY63_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99928.1"
                     /translation="MWKKIRRGLKVFLVTILITAGLIIAYFATIITINSNNNFSPPVT
                     DTLIISEGRNAWLFADENISMISWNIGYCGLGKEMDFFYEGGKKMRPDKDLYKSYLNA
                     VFNIVSAMDSVDFLLLQEVDFDARRSFYSHQDSSLMQALPDFSSAKAVNYDVPYVPFP
                     LFNPMAKVYAGMMTFSRYIPEWAQRISYPGSYSWPMSVFFLDRCFIIMRYEIDGEKDL
                     YVINTHNSAFSDAGDIRVAELLFLRSFVLDLYNRGHYVIAGGDWNQNPPGFNPAEIKT
                     GDKVRLVGTSVSNDFLPEGWKWMYDSSTPTNRDVNISYQKGTTTTTIIDYFLCSPNIE
                     VNEVKTIDMGFTHSDHNPVYLNFALIPDSACCDSMKIYETMKEKIATEKKKKIKKN"
     gene            1284..2063
                     /locus_tag="PKY63_04605"
     CDS             1284..2063
                     /locus_tag="PKY63_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPE99929.1"
                     /translation="MRFFFVVFSSLLFFISSFSALSQDDWKDWMLHKTFETSQGVLPY
                     RMYVPDSIGPDEKLPLVLFLHGAGERGDDNEAQLTHGVRHFMNDSIRMQFRCIILAPQ
                     CPANARWVEVDWSNASHKMPARISVPLFQTFTLLDSLIAALPVDTLRVYITGLSMGGF
                     GTWDALARRPDFFAAAMPVCGGGDEGTACLMKKVPVLAFHGKLDHLVIPSRTTNMVNA
                     VNQCGGNAQFVLFPKLGHLCWEQVYSNVENIRWLFFQKRQK"
     gene            2085..2885
                     /locus_tag="PKY63_04610"
     CDS             2085..2885
                     /locus_tag="PKY63_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99930.1"
                     /translation="MKFFNSQKPPRDYLQYVLLLLGSLLLFSVLSFSNAEISIGSYKL
                     KDSGIRKFFYPEVLPLAQTGISIEQDGTGDKVDSSAQRFLLIGDSMLEFLRIRLGDYA
                     RKNGHTVNTVIWYSSSSLWYGQCDTLKHFINKHKPTYVLLVLGANELFIKDITSQRAE
                     YVRNIIAQMDTLPFVWVGPPNWKDDTGINDLILRYAGKDRYFPSKNLSYERTKDGAHP
                     KRESAYKWMDSVAVYLQTEARYKILMTSPDTFLNKVPPTEILQPNPPF"
     gene            2885..4363
                     /locus_tag="PKY63_04615"
     CDS             2885..4363
                     /locus_tag="PKY63_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPE99931.1"
                     /translation="MNWSNIIHDILFYKPAAPILFNSGTFWLLFILVLSVYAAIYRNN
                     TARTLFLLGFSIFFYYKSSGVYVMLLIGTLVFVYMLTKLMFRQKNPVLKKWMLVVNVV
                     VTLGFLAYYKYTNFFIDNFSLISGQQFEAADIFLPVGISFYTFQILSYVFDVYRGDLE
                     PEKDFFNYAFYITFFPQLVAGPIVRASQFLPQLKGHITIHPDEVQKGFFLIMQGLFKK
                     AVLADYVSQYNDLIFNAPGTYSGFENLMAVYGYTLQIYCDFSGYSDMAIGIGKMMGFD
                     LGINFNKPYQSLSITEFWRRWHISLSSWLRDYLYIPLGGNRKGKLRTYINLFITMLLG
                     GLWHGPSWQFVFWGGMHGVGLAIHKLWKTYVMPGEIDSKWGSRIYTFFAWFITFHFVV
                     FLWIFFRAQSFELAWEMIGQIFGAMDWAYLQPFWSVRHIFVVMLIIGIAIHAVPSRLF
                     PKMENIYVKMPFVVKAIAFVVLIQLVIQFKSESVQPFIYFQF"
     gene            complement(4454..6196)
                     /locus_tag="PKY63_04620"
     CDS             complement(4454..6196)
                     /locus_tag="PKY63_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HPE99932.1"
                     /translation="MKELKIRDLTLRDGQQSLFATRMNQAQVDHVLPLFKEAGFYAME
                     VWGGAVPDSIMRYLNEDPWERLAKIKDAIGTSSKLTALSRGRNLFGYNPYPEHVIEGF
                     IKNAMSTGIDILRIFDALNDMDNIRSSVGFAKKYGGIADGTISYTVDPKLELKKKFFG
                     LIKEKPKPPVFTVDYFVGKAKEIEAMGADMITLKDMAGLVDHVSAAKIIRALKNNVKI
                     PVDFHTHSTPGYGLASVLTAMMNGADIIDTNMMPFAGGSAAPAYEIIHLFCKKLGYET
                     NVNIEVLKTLSDKLEEIRHQMDEFDSYKMFPRSFDLTKDKLPTDIEKVFDRAINSVEK
                     ENYEDVLEACHEIETYFNFPAPNEMVKNAQIPGGMYTNMLAQLKTLKLEELLPRVLKA
                     VPAVRTAAGSVPLVTPTSQIVGVQAVNCILDENKGLPWYTNVNTQYVNLVKGSYGKTP
                     VPIDPDFREKITGAREEIPYNTDNYKPQVNKVLSQFGDRNLVNSEKDQLLLELFPAVA
                     EPFLTNRAKKEWDAEQAIVKAEQDAIRAEEERKREEERAAFEAEKAAYKALSKEEKEK
                     RLLDGLYNYKWFTF"
     gene            6328..7998
                     /locus_tag="PKY63_04625"
     CDS             6328..7998
                     /locus_tag="PKY63_04625"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPE99933.1"
                     /translation="MKSDIQIARETSIKPITEIAKQLGINPDDLDLYGKHKAKIPLSY
                     INDEKIKKSKLILVTAITPTPAGEGKTTTSIGLAMGLNRIGKKTTVVLREPSLGPVFG
                     VKGGAAGGGYAQVIPMEDINLHFTGDMAAVEKANNLLSALIDNNLQNTKNSLNLDPRT
                     ISWKRVMDMNDRSLRDIVIGLGGTANGIPRETGFNITAASEVMAILCMASDKDDLEKR
                     LGNIFIGFTFDKKPVYARDLNAQGAMAVLLKDAIMPNLVQTLDNTPAIIHGGPFANIA
                     QGTNSIIATKTGLSLSEYVVTEAGFASDLGAEKFFDIKCQTAGLMPNAVVIVATVRAL
                     KYHGGAKKDQFAIPDAALVSKGLPNLEKHIENMKKFGFTPVIALNKFDTDSVEEIKVV
                     EDFCKTQDVSMAVNDSWARGAEGALELAEAVVSEAKSCPACFKPLYDKNWDVEKKIST
                     IVTNMYGAKDVEYSLKARKDLQNILSLGLDKLAICMAKTQNSLSDVPELRGRPKDFTV
                     NIREIEISSGAGFVVPIAGSIMRMPGLPAVPSAENIGFDDDGNVTGLF"
     gene            complement(8189..8605)
                     /locus_tag="PKY63_04630"
     CDS             complement(8189..8605)
                     /locus_tag="PKY63_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99934.1"
                     /translation="MKQKQYPTDVLIDSCVEDVKSRYQTEERLVKFSVSILDFCHDFP
                     QIHIAEYFADQLSRASVSASLNYGEAQAAESTKDFIHKMKISLKELRETLICLKIINR
                     SKYFSQIKDGQIIQKEAEELVAIFVASMRTIHAKQN"
     gene            complement(8770..9375)
                     /locus_tag="PKY63_04635"
     CDS             complement(8770..9375)
                     /locus_tag="PKY63_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPE99935.1"
                     /translation="MGLWHITESVDELISMLFLNKEEFDTLASFGNDTRRRQWLSYRA
                     LIRQLVKTDYIYRIYYDDDNKPFLVNPQSSISITHCTDYSAVLISPNMKLMHGLDIEP
                     IHPKILKVTQKFMDAEEHEEWKKTENLEKAVMYWTFKEAIFKAYGKRYITLRGNIHIN
                     PFETERAKAHGFIRLKSKQFPFDLHLRQFQNLLISVAHEPE"
     gene            complement(9427..10467)
                     /locus_tag="PKY63_04640"
     CDS             complement(9427..10467)
                     /locus_tag="PKY63_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPE99936.1"
                     /translation="MKKWILIWVCLVPLFAIAQAPTGYYDDAEGLTGSALKTALHNII
                     STGYTITVYDDLWDVYPITDKKSNGKVWDMYSDIPGGTPPYEFTFVTDQCGNYNSEGD
                     CYNREHSVPQSWFGGSLPMYSDMFHVVPTDGKVNGQRSNFPFGEVSSASWTSQNGSKL
                     GPCGFPGYTGTVFEPIDSFKGDFARIYFYMCVRYKDEMPSWSGESFSGGDLSSWTENM
                     LLQWHALDSVSTKEIERNNDVYGIQHNRNPFVDNPDWVYSIWGPTSGLENGFQQIVSI
                     FPNPADNNISIRYATSERPEHVYLCDLQGRVITEEITENLIETGHLPEGFYFLKFIFK
                     SGITEKEFVIMH"
     gene            complement(10711..13500)
                     /gene="polA"
                     /locus_tag="PKY63_04645"
     CDS             complement(10711..13500)
                     /gene="polA"
                     /locus_tag="PKY63_04645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPE99937.1"
                     /translation="MEEKKPVLFLLDAMALAYRSYYAMIKNPVRNSKGLNTSAILGFG
                     NTILDLINKEKPHLLGVAFDTIAPTARHDEFAFYKANREPMPDDLSISLPYIKEMLQL
                     MDIPILEVDGYEADDVIGTLAKEAETRGYQTYMMTPDKDFGQLVSENIFMYKPAKFGA
                     AAEVWGVREVCDRYGIERPEQVIDILAIMGDAADNIPGIFGIGEVGAKKLISEYGSVE
                     NLLAHADEIKNPTMKQKVLAGKESALISKQLATIITDVPIQFMPSRLEWKVAPFAPLQ
                     KFMEELEFKTFSKRWWDAMGIIAEKTPAVAQGQSSLFDQDFVEARAGLDSIISNINSN
                     EHEYKLIDNTDDLEKLVSELKKEKLICFDTETDSLDTLVATLAGISFCTEPGKAFFVY
                     LKDEDFCKSVLEMLKPIFENEKIIKIGQNLKYDIAVMRTHGIHIKGKLADTMLAHYLL
                     QPELRHNLDYLSENYLNYKTITYEELLGSGKQKISIFDVTAEKLKDYACEDADITLKL
                     WKIFEPELDISGMTELFYEIETPLIEVLEDMERSGVRIDQQSLKEFSQLLGKEILQLE
                     SDIHQLAGQPFNIASPKQLGEILFDKLQIAEKPKKTATKQYSTSEEVLQKYISRHPIV
                     AKVLEYRGLSKLQNTYVDALPELIHPETGRVHTSYNQAVTSTGRLSSTNPNLQNIPIR
                     TELGQEIRKAFVPRDENHVLISADYSQIELRIIASISGDEHMVDAFRQGIDIHTATAA
                     KVYGVALNDVKPEQRRHAKMVNFGIIYGISAFGLSERLGISRKDASDIIDNYFEQFPG
                     IKKYMDDTILFARANGYVETLKHRRRYLKDIHSTNSFVRGFAERNAINAPIQGSSADM
                     IKMAMIDIFDWLKKSNLKTKMILQVHDELVFDVPKEELEIVKPEIEKLMKAALPLKVP
                     VEVEVKDGRNWLEAH"
     gene            13875..15467
                     /locus_tag="PKY63_04650"
     CDS             13875..15467
                     /locus_tag="PKY63_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99938.1"
                     /translation="MKKVLTVVLGLIMSAGIFAQHSIENPFFDQVTYRGAFDATTLWT
                     DGWTNWDAENTTYPATTVTKSGEITSNETWTASDVYKIEGFLYVRSGATLTIEAGTVI
                     RGDKASKGTLIVERGAKLNAVGTSANPIVFTSNEAAGSRDYGDWGGIVLCGKAAINVP
                     GGEAQIEGGPTSYYGGGTSPDNADNSGTLSYIRIEFPGIPFVTDKEINGLTLGGVGSA
                     TTIDHIQIYRCGDDAFEWFGGTVNAKYLVSSFTWDDDFDTDYGFSGMIQYAVALRDSS
                     IADPGSGSNGFESDNDGSGSANTPVTNPIFSNISMFGPKVLSTTSINTNYKRAMHLRR
                     NSQIDIYNSFFAGYKDGLFVDGSAARQNLAEGELSLFGVTLAYCSGNYFPEDTVNVVR
                     DYYLTSWRMNDTLTDHALLQIADPFNYGNPDFRPVTGSPALNGSVFSGIIDRESNNVD
                     ELIIYPNPATNVLNVAFSLRDDETVVVEIMDMSGRICSRVVESREMGSNVVGIDVHQL
                     ETGVYSVRIKAGSSIATMRFFK"
     gene            15665..18520
                     /locus_tag="PKY63_04655"
     CDS             15665..18520
                     /locus_tag="PKY63_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPE99939.1"
                     /translation="MKKLFLFFIISFSFSFLFSQTSFQSEVPVRQGTMDVKGRVVDAK
                     TNEPVIGANVVLKGTTLGASTDLDGNFVLPAIPKGNLTVTVSYVSYKPFEHTFYISGE
                     KNLFLDIKLEENITQIQTVTVVSNRVTGTEVSMMQTIRQNNLVVNGVPSQMIKKTQDR
                     DAAEVVKRIPGITVQDGRFVIIRGLNERYNSVWLNDLPAPSAEADVRAFSFDLIPASM
                     IDNVVVYKTFSPELPGDFAGGLVQISTVSNPEKNHLQISYSNGYAAGSTFQQHLLYPV
                     EGSGITNATSSLKLPQLFPENVSTIPSTEAGKDLKTFWGQTMNKIWTPHSITAPTDQR
                     FSVSGAWSKKWDNARLVNFSQINYSSTFQQDSIFRASYNAYDEIGQKPDTNYYFNDVQ
                     YVSSVKAGAMMNWSLIKGKSTYEFRNLLNYQSSNRVTEREGRDFYGGIYIRGLEMAVK
                     QRHLYSGQLSGTHSFGKKESKIKWFAGYSVSSQQMPDTKRLTWVLNDQEESPYCGEYG
                     LNFSFTANSNLSGRIFHDMNEQIVNAGSDFNSYISAGSSKIAYKTGFFYENRNRLFNA
                     RNLGYKIAKTSLFNWDLPYQSVENVFGNENINAADGIMLDEQTSPSDSYTSHSELLAG
                     YFTLTLPVGSRIKIHTGIRAEHSRVSLHSYTADATNPPSTEDEIHYLSDTVLFLPAIN
                     FSFSMTENSLLRLAYGQTLNRPEYREIAPMSFYDYEMKAVITGNDSLTFASIHNFDFR
                     YEYYPNAYSMVSIGAFHKQFINAIEYQIIPTGSGLQYSFQNTPEASVSGLEAEMRLSA
                     ASLKKFDYLKNFTLIFNAAYMKSRICFEENSLEEDRALQGQSPFILNASLFYQNDSLG
                     LNAGLMFNIVGKRISFVGDPFTGNPHIYEMPASKLDLSISKNIGKKIEVKAQVRNLLN
                     SASRYAQNIETESGTIEQTTMQYNPGRYYLLGLVWKL"
     gene            18612..20159
                     /gene="dnaB"
                     /locus_tag="PKY63_04660"
     CDS             18612..20159
                     /gene="dnaB"
                     /locus_tag="PKY63_04660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPE99940.1"
                     /translation="MEENKDKKNTKRSVKPAPLKASVLDHGRVPPQAVELEEVILGAL
                     MLEQNALNTVIDIIQLDSFYKEAHRYIFEAIQSLFARSSPIDMLTVIEELKKQGKLEL
                     VGGAYYITYLTNRVVSSANIEYHARIVAQKYIQRRLIEISTEIINDSFEESTDVFDLL
                     DSAEDKLFKINEENLRRKTNGMAQLLRSAREMVESASKNENSLSGVPSGFKSLDAVTA
                     GWQRSDLIIIAARPGMGKTAFVLSMARNMAMINEVPVALFSLEMSAIQLVMRLIASEA
                     RISSERLRTGKLTAQEWKSLNDNMDNLSKAKLYLDDTPALSVFELRAKARRLKQQYDI
                     QCVIVDYLQLMTSKVEKNANREVEIATISRSLKALAKELNIPVICLSQLSREVEKRPG
                     SKRPQLSDLRESGAIEQDADIVMFIYRPEYYFKEDDKNTDIETIRNKAELLIEKHRNG
                     PLTTINLKFLGAYARFYEENDEDFSHAIRILSESGQTSSGSSFTVQSKMNNDHSGDDF
                     SIRKSDL"
     gene            20193..21458
                     /locus_tag="PKY63_04665"
     CDS             20193..21458
                     /locus_tag="PKY63_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPE99941.1"
                     /translation="MKTRYFLVLFVLIFLPRLADATHLLIPMDQSQKNHLKAYGITYW
                     ILANNEEAEWLLNYRGGSFMCGFSKTIQDECNIRGVSVEVISDAQAASIRAEIADPSV
                     NMDIIQLTKAPKVAVYAPKTNLPWDDAVTLVLTYSEIPYEQIYDEEVLSGDLPKYDWL
                     HLHHEDFTGQYGKFWAHYQNTDWYKQDVAESEKSAKALGFTKVSQMKLAVAKKIRDFV
                     AGGGYLFSMCSAPDSYDIALAAEGVDICASQFDGDPMDPQAQSKLDFSKTFAFENFKI
                     STNPYEYEVSNIDVSLSRRAMNPRDDYFALFEFSAKWDPVPTMLCQNHERIIKGFMGQ
                     TTAFNSATLKSSVLVMGEFKAIGEARYIHGEYAKGTWTFLGGHDPEDYQHFVYDPPTD
                     LNLFPNSPGYRLILNNVLFPAAKKKLQKT"
     gene            21632..23290
                     /locus_tag="PKY63_04670"
     CDS             21632..23290
                     /locus_tag="PKY63_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPE99942.1"
                     /translation="MRIFTFLILLMMVLGSFAQLSIEGSPKSFKSFGTHSVVPFVDLQ
                     KPDMTVVNAQDEAADMAGKAYRNGILLQTEITPETSGVWDYLPDGSAIWRVGVKSDNA
                     IALGLYFNDWYLTPGIEVYVYTPDKSFVIGAFTELNNIDLRTMATQEIPGDEAILEVF
                     VPDRDQKFDLALNQVAYFYRGLNIVKNVKASDDCEVNVICSEGDNWRDQIRGVAKISM
                     ILSDGAYMCSGSLVNNIAQDCTPFFLTADHCSVSQSGVVVTATQLSQWTFRFNYQATT
                     CTGSTSGTSKVYTGATYKASDTYGETSTGSDFFLCQLNNTPEATYNPYYNGWSYLNTA
                     GTSGVSIHHPAGDIKKISTYTATLINYTTHWGVTWVTTTNGHGVTEGGSSGSPIFDQN
                     KRVVGTLTGGASACEVNGAGSGTGPDEMDVYGKMSKHWTGVGTTSDKNVKYWLDPNNL
                     SGGTLAGRNYCVAGLDDVAYIRANIHLYPNPATDYLSIDFSDYNVENGTIVVTDMLGK
                     TVTAPVNGDFTGSESIDVRELEPGMYFLMIQSQSTSVSLPFVVE"
     gene            23357..25186
                     /locus_tag="PKY63_04675"
     CDS             23357..25186
                     /locus_tag="PKY63_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPE99943.1"
                     /translation="MAFYSLSEIIDLLRKRSLVAEVGEEVSPILEASEIADRFASDEK
                     SGRPLLFLQNGTAFPLLMNLFANKEITDELLNLTGTEGYFSRLQNLLSMMDSKPRLSR
                     KSIRSLMGLRKTAPKKLHHKGACMAVVQPAPDLNLLPVIQCWPYDGGRFLTLPMVHTI
                     DPDTGKQNTGMYRMQVFDGQSTGMHWHRHKGGAAHFEAWKKKGKRMPVTVTLGGDPVL
                     TYAATAPLPEGISEYMLAGFLKNKRVPLVKSHNSDIWIPECSDFVIEGYIDPSEPLRT
                     EGPFGDHTGFYSLPDAYPVFHITRISHRKKAVYPATLVGVPPKEDQFLGHATSQIFFP
                     LIQKIFAPEILDLYLPVEGGFHNLALVKIRKKFPGQAVKVMHALWGAGQMMFTKCIMV
                     FSEEVDIRNPHAVLDAIDKNFSPVQDLHMSAGPYDVLDHAAQSFSHGGKAGFDCTLKT
                     EERKNSPTGNTEDKSDSMRLFGKSIHVIFSDHAVAESGNLFLNLAGGAAPVRKGIYII
                     TDTCMKEAPDNILLWYVLASIDPARDFSLIRSLPAEGVLVINACVNGKRAVPGGRWPA
                     ATVMSDEVVSLVNEKWESYEVGDFIESPSTRLSELKSDSYLNR"
     gene            complement(25227..26513)
                     /locus_tag="PKY63_04680"
     CDS             complement(25227..26513)
                     /locus_tag="PKY63_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPE99944.1"
                     /translation="MEEKLPEIYTEEVHDILSKPPSGIIRNGVTLIGIVIILLLAGSW
                     FIKYPDKVTAAIKVSTTQVPASLIAKTSGKITHLFVSNNQMVHKGDILAVIENNAEYN
                     DVILLDSLLLTFDVNNDSALENIALVPALLLGDLQSDFTMFQKSVSDYLLYKSVNLLS
                     KKITALERQLSLTDQYYRRLSDQSNLQGEDLQIAQNEFRRDSILFSQKVLSAAEFDKS
                     RASLIQRKQSYMSSRTNLSSTQIQKAQIEQQVVEMQLQQADESKQYIQSIQQSQKTLS
                     NSINIWKNTYLIQSPIDGKVTFTNTREVNQNVVSGDVVMTVVPEKKSELVGTIVLPMQ
                     GAGKVKQGQQINIKFDNFPHMEYGMVKGMVKSISLVTTESNYIVEVAFPDGLRTNYNQ
                     ILNFSQEMSGSAEIITDDLRLIERFLNPIKSIIKNQ"
     gene            complement(26624..27007)
                     /locus_tag="PKY63_04685"
     CDS             complement(26624..27007)
                     /locus_tag="PKY63_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPE99945.1"
                     /translation="MAEEKKYLDLNDLSSYRLAMEMGTLVWDIVNEWEYFAKDTIGKQ
                     FVRCVDSVAANIAEGFGRYGKRSRIVFFRYAYGSLYEARAWAQKAFDRKLITEDQLFK
                     IRKFLEALPVEIHQLIKFTNERLKF"
     gene            complement(27351..29537)
                     /locus_tag="PKY63_04690"
     CDS             complement(27351..29537)
                     /locus_tag="PKY63_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HPE99946.1"
                     /translation="MRVPIYLQPDSRDCGPTCLRMVAKHYGRNYSLAYLREKSSITRH
                     GVSMLGISDAAESIGFKTLGVKVSFEQLKEEAPLPAIVHWRQNHFVVVYDITDKAVFV
                     ADPASGLIKYTKEEFEKGWISTKSDDENKGIALLLETTPDFYEFKGEKSDRQKFSFLF
                     KYLTPHKKYIVQLFLGMLLGSLLQLILPFLTQSVVDVGIGNQDLAFITLILISQLVLA
                     ATQMSVDFLRNWILLHISTRINISLISDFLIKLMKLPIGFFDSKLIGDIMQRIGDHTR
                     IQNFLTGSSLSTLFSMVNLVIFSAVLAWYDLKILGIFLFGNAMYIAWILLFMKKRREL
                     DFKRFALAADNQSTLFQIITGMQEIKLNNCEKQKRWRWERLQAAQFKVSIKGLALGQY
                     QQVGSLFLAQATNIFVSFLAARAVVNGQMTLGMMMAVSYIIGQISSPINQLIGFIQSL
                     QDARISLERLGEIHNKPDEEPEEMNRVAILPENKSLHVNNITFRYEGPNSPSVLENID
                     LDIPEGKITAIVGTSGSGKTTLIKLLLGFYKPEQGEIKVGDLSIEHVNGSFWRSQCGV
                     VMQDGYIFSETIAENIAPGDDDIDRRKMLHAVKVANIREFIEGLPLGYNTKIGQEGSG
                     ISQGQKQRMLIARAVYKDPQFIFLDEATNSLDANNERVIMDNLNEFFKGKTVVVVAHR
                     LSTVKNADQIVVLEKGKIIERGNHAELTEKRGSYYELVKNQLELGN"
     gene            complement(29557..30018)
                     /locus_tag="PKY63_04695"
     CDS             complement(29557..30018)
                     /locus_tag="PKY63_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99947.1"
                     /translation="MDSISHHSRVKLNYKYTDCSRIAYIQITDPLVDTTCNWIIPDRE
                     IDTRGRLFLSREYKPETRSFLFFSQVLNELLKNEKAKVVFHCYTQFDESDLANADQIT
                     AMCNGIVSYIEWDGFSDDRVMYVIHWSQKPSVVKPDSPINKISIVFSFIEN"
     gene            complement(30421..31344)
                     /locus_tag="PKY63_04700"
     CDS             complement(30421..31344)
                     /locus_tag="PKY63_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99948.1"
                     /translation="MEMKANLFVFSIFLFFTSITLFCQTDTIVINDTIMVIDNSIPEY
                     IDAFYKADYSTCVSEAEELLKNSSIDGFLYYYLLNSYASLGDSVGFLRVAGTVSPEVD
                     ILFIFRDEKIRTMLGEKVFTDLQKPFIADAINRCSNADFDYMCILSDCYLNTSRYRSL
                     YRFYFSDQEKMKTFLDAQSLLDSLNFFMLQQTIKSKGLPTQNKVGKTGMNQYMLCVQH
                     FSKANFVNTKKDIRKLYRRGEITSLDYAYYIDRYKVYRNKKQIFATQLMRDSYGALIF
                     QPVRKPKSVDKRRRKMGFDTTAEEYLKMISN"
     gene            complement(31335..32330)
                     /gene="gwsS"
                     /locus_tag="PKY63_04705"
     CDS             complement(31335..32330)
                     /gene="gwsS"
                     /locus_tag="PKY63_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system SPASM domain peptide
                     maturase"
                     /protein_id="HPE99949.1"
                     /translation="MKNYFILFPFVKIINGVARNILYDSQRQNIKFISGVEVEILHNL
                     AKHDIDTVALRYSKKTLNDLINNYVETGFAIVTSGINSRKMLKLSELYDSPNLIENAI
                     IIIHENTNSSIYSEAINQLSELNCRVISIRMYSENASLLHLERCISKIIESHILHCEL
                     LLPIDSQSRVYSLICDNNHYHVDFSIFYFGCKKSDYIPGPPNQYFIKGKLTKNDCGVF
                     HPSLLNLDYSHFCLSKSFNSCLFKKVVIDEDGNIKNCPLFTQSHGKIFEDSLADIITL
                     QKFQDWWHIKKDDISVCCGCEFRYFCHDCRAVTVEDGFFEKPLNCSYNPKTTKWK"
     gene            complement(32500..33492)
                     /gene="gwsG"
                     /locus_tag="PKY63_04710"
     CDS             complement(32500..33492)
                     /gene="gwsG"
                     /locus_tag="PKY63_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system ATP-grasp peptide
                     maturase"
                     /protein_id="HPE99950.1"
                     /translation="MILILTERNDKSVFRVCEWLEFLNTKYTLIFDEDTLDILSLNVS
                     DGGVKFVYKVRGEIVDYDLISAVWWRRTDFFLKKVIDDNKDENIRSIKLMEIDEFQSY
                     NAFIHYLFSLKPSIGNRLKYPINKLQSLIVASKNGIKIPTTSICYNKKQLKEKMECYN
                     VITKPVKDFLPYEHNDQFHILYTQPINVDTLANIPEQFNYSLIQNCVEKNFEIRAFVF
                     GDMIFSMAIHSQFSPSTKNDFRTYEEWVPYTPYKMPINIEKKILSFMKDMNLETGSLD
                     FIVSMDEEIIFLEVNPVGIFDMVSVPCNYNIEKKIAEYLIKIQQLSNSQEFVMS"
     gene            complement(33563..33775)
                     /locus_tag="PKY63_04715"
     CDS             complement(33563..33775)
                     /locus_tag="PKY63_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99951.1"
                     /translation="MKKNIENFELACILNGQEHILGGICSERTTWTSGQSSGTDTKYY
                     DPTYGSSDPTIITDETNVSRVYDSPK"
BASE COUNT         9480 a         6784 c         6914 g        10696 t
ORIGIN      
        1 gtgacgcatg cgcctgcaac tcgctgaatg acagttttca tgttttaatt tttctttatt
       61 ttcttctttt tttcagttgc aattttttcc ttcatggttt cataaatttt catagaatca
      121 caacaggcac tgtctggaat caaagcaaaa ttaagataca cggggttgtg atctgaatga
      181 gtaaaaccca tgtcaattgt tttcacttca ttcacttcaa tgttgggtga acacaggaaa
      241 tagtcgatga tggtcgttgt tgttgtgcct ttctgatatg aaatattaac gtcgcggttg
      301 gttggtgtgc tgctgtcata catccatttc catccttccg gtaaaaaatc gttgctcaca
      361 gaagtgccta ccagtcttac tttatcacca gtcttgattt cagccggatt gaaccccggc
      421 ggattctgat tccagtcgcc accggcaatt acataatgtc cgcggttgta cagatcgagt
      481 acaaaactac gcaggaacaa aagctctgca actcggatgt cgcccgcgtc gctgaatgcc
      541 gaattgtgcg tgttgattac atacaagtct ttttcaccat caatctcata tcgcattatg
      601 atgaaacagc gatcaagaaa aaacacgctc attggccagg agtaactgcc aggatatgat
      661 attctttgtg cccattcggg aatataacgc gaaaatgtca tcatgccggc atatactttt
      721 gccattggat tgaaaagcgg aaacggcaca tagggcacat catagttcac ggcttttgcc
      781 gagctgaaat caggcagtgc ctgcatcaaa gagctgtcct gatgtgaata aaaagagcgt
      841 cttgcatcaa aatccacttc ttgcagaagc agaaaatcaa cactgtccat ggctgaaacg
      901 atattgaata cggcattcag atacgatttg tacaaatcct tgtctggccg cattttttta
      961 ccgccctcat aaaaaaaatc catctctttc cccagtccgc aatagccaat gttccatgaa
     1021 atcattgaaa tgttttcatc tgcaaatagc catgcattgc ggccttcgct gattatcagg
     1081 gtgtcggtta caggtggcga aaaattgttg ttcgaattga tggtgatgat tgttgcaaag
     1141 tatgcgatta tcagccctgc agtaatcagg attgtaacca gaaaaacctt tagtccccgt
     1201 cggatttttt tccacataat tcaggaataa tttttgtaaa aatacatttt ttaaaatatt
     1261 cacgatattt gtaaaaaatc ctgatgcgat tcttctttgt tgttttttct tctttgttgt
     1321 tttttatttc ctcattcagt gctttatcgc aggatgactg gaaagattgg atgctgcaca
     1381 aaacatttga aaccagtcag ggtgtgttgc cttatcggat gtatgttcct gattcaatcg
     1441 ggcccgatga aaaactaccc ctggtgctgt ttcttcacgg ggcaggtgag cgtggcgatg
     1501 ataatgaagc acagcttact catggagtca gacattttat gaatgatagt atccgcatgc
     1561 agtttcgttg tatcattctt gcccctcagt gtcccgcgaa tgcacgatgg gtcgaggttg
     1621 actggtccaa tgcttcacat aaaatgcctg cccggatttc ggtgcccctt tttcagacat
     1681 ttacgctttt ggactcattg attgcggcat taccggtcga tactttgcgt gtttatatca
     1741 caggcctttc gatggggggc tttggaacat gggatgcgct tgcccgacgg cctgattttt
     1801 ttgctgctgc catgccggta tgtggaggtg gagatgaggg aacggcctgt ctgatgaaaa
     1861 aagttccggt tttggcgttt catggaaaac ttgatcatct ggtgattcca tctcgtacta
     1921 ccaatatggt gaatgctgta aatcaatgtg gtggaaatgc tcagttcgtt ttatttccca
     1981 aactgggaca tctttgctgg gaacaagtgt attcaaatgt tgaaaacatc cgttggttat
     2041 tttttcagaa acggcagaaa taattatttt tgccgcaaaa agttttgaaa tttttcaact
     2101 cacaaaaacc gccacgcgat tatttgcagt atgtcttgtt attactgggc tcattgttgt
     2161 tgttctctgt tttgtctttt tccaatgctg aaatcagcat cggttcttat aaactgaaag
     2221 attccggaat ccgcaagttc ttttatcccg aagtgcttcc attggctcaa acaggaattt
     2281 ctattgaaca ggatggtact ggcgacaaag tggattcatc ggcacagcga tttttgctga
     2341 taggtgattc catgcttgaa tttcttcgta tccggctggg tgattatgcc cgtaaaaacg
     2401 gccacacggt caatactgtt atttggtata gctcttcgtc gctctggtac ggacaatgcg
     2461 acacattaaa gcattttatc aacaagcaca aaccaacgta tgtattgctt gttttgggag
     2521 ccaatgagct ttttatcaag gatatcacct ctcaacgggc ggaatatgtc agaaacatca
     2581 ttgcacagat ggatacgttg ccttttgttt gggtcggacc tcccaactgg aaagatgata
     2641 caggaatcaa tgatcttata ttgcgttatg caggaaaaga ccgttatttt ccatccaaaa
     2701 atctttcgta tgaaagaacc aaagacggtg cgcatccaaa gcgcgaatct gcctataaat
     2761 ggatggattc tgtagctgtt tatctgcaga ccgaagcccg ttataaaata ctgatgacat
     2821 cccctgacac atttctcaat aaggtgcctc ctaccgaaat tctgcaaccc aacccgcctt
     2881 tctgatgaac tggagtaata ttattcacga catacttttt tataaacctg ctgcgcctat
     2941 tttgttcaac agcgggactt tctggttgct gttcattctg gttctttcgg tgtatgcggc
     3001 catttaccgc aataatacag cccgcacatt gtttttgctt ggatttagca ttttctttta
     3061 ttacaaatcg agtggtgttt atgttatgtt gctgatagga acacttgttt ttgtgtatat
     3121 gctcacaaaa ctgatgttcc ggcaaaaaaa tccggtttta aaaaaatgga tgttggttgt
     3181 caatgtggtt gttacacttg gttttcttgc ctattataaa tacacaaatt tctttattga
     3241 caatttttcc ctgatttcgg gtcagcagtt tgaagcagcc gatatttttc tgccggtagg
     3301 aatttctttt tatacttttc agattcttag ttatgttttt gatgtgtatc gcggcgatct
     3361 ggagccggaa aaagattttt tcaattatgc gttttatatc actttttttc cacagcttgt
     3421 tgccggccct atagtcaggg cgagtcaatt tctgccgcaa ttgaaaggtc atattaccat
     3481 tcatccggat gaagtgcaga aaggtttttt tcttattatg cagggcttgt tcaaaaaagc
     3541 tgtcctggcc gattatgtgt cgcagtataa tgatttgatt tttaacgctc ccggcactta
     3601 tagcggattc gaaaatctga tggcggtata tggttacacg ctgcagattt actgcgattt
     3661 tagtggttac agtgatatgg ccattggaat tggaaaaatg atgggatttg atcttggaat
     3721 taatttcaat aagccatatc agtcgcttag tataacggag ttctggcgtc gatggcatat
     3781 cagtctttca agctggttgc gcgattatct ctatattccg cttggtggaa accgcaaagg
     3841 aaaactgcgt acttatatca atctttttat caccatgttg cttggtgggc tctggcatgg
     3901 gccttcgtgg cagtttgttt tttggggtgg tatgcatggt gtaggactcg ccattcataa
     3961 attatggaaa acctatgtaa tgccaggcga aatagactca aaatggggct cgcggattta
     4021 cacatttttc gcgtggttca ttacttttca ttttgttgtt tttctctgga tatttttccg
     4081 ggctcaaagt tttgaacttg cctgggaaat gatagggcag atatttggcg caatggattg
     4141 ggcatatttg cagccattct ggagtgttcg ccatatattt gttgtgatgt tgataatcgg
     4201 tattgctatt cacgccgttc cttcacgttt gtttccgaaa atggaaaaca tatacgtaaa
     4261 aatgcctttt gttgttaagg ccatcgcttt tgttgtattg attcagcttg taattcagtt
     4321 taaaagcgaa tcggttcagc cgtttatcta tttccagttt taattacaac cttttaaatt
     4381 tctttaaact tcggcaaagt ttaaactttg ccgaagttaa gaagttaaaa agaagtttgt
     4441 ttaaaggttg taattaaaac gtaaaccact tgtagttata caagccgtcg agtaaacgct
     4501 tttctttctc ttctttgctg agggctttgt aggcggcttt ttcggcctcg aaggcagcac
     4561 gttcttcttc gcgtttgcgc tcttcttcgg cacggatggc atcttgttcg gctttgacaa
     4621 tggcctgttc tgcatcccat tcttttttgg cgcggttagt cagaaatggc tctgctactg
     4681 ccggaaacaa ttcaagcaac aactggtctt tttctgaatt taccagattg cgatcgccaa
     4741 attgcgataa aaccttattg acctgcggtt tataattgtc ggtattgtac ggtatttctt
     4801 cgcgcgcgcc ggttattttt tcacgaaaat caggatcaat aggcaccggc gttttgccat
     4861 aggagccctt aacgagattt acatattgag tgtttacatt ggtgtaccag ggcagacctt
     4921 tgttttcgtc gagaatgcaa ttaacggcct gaactccgac aatctggctg gtgggtgtaa
     4981 ctaaaggcac agagccggct gcagtgcgta cggccggaac agctttcagt acgcgaggta
     5041 aaagttcttc gagtttcagt gttttgagct gagccagcat gttggtgtac atccctcccg
     5101 gaatttgtgc gtttttcacc atctcgttgg gtgccggaaa attgaaataa gtttctattt
     5161 cgtggcaggc ctccagaaca tcttcgtaat tttctttctc aaccgaattg atggcgcgat
     5221 cgaaaacttt ttcaatgtcg gttggcagct tatctttggt gagatcgaag ctgcgtggaa
     5281 acattttgta actgtcgaat tcatccattt ggtgcctgat ttcttcaagc ttatcgctga
     5341 gggttttcag cacttcaatg ttgacattgg tttcatatcc cagttttttg caaaacaaat
     5401 ggatgatttc ataagcgggt gcagcactgc cgcctgcaaa aggcatcatg ttggtgtcga
     5461 tgatgtcagc gccgttcatc atggcagtca gtaccgaagc cagcccgtag ccgggagttg
     5521 agtgtgtatg gaaatcaacc ggaattttta cgttgttttt cagtgcgcgg atgatttttg
     5581 ctgcacttac atggtcaaca agaccagcca tgtctttcag cgtaatcata tctgcaccca
     5641 tggcttctat ttctttggct ttgccaacaa aataatctac cgtaaaaaca gggggcttcg
     5701 gcttttcttt aatcagtccg aaaaatttct ttttcagttc gagctttgga tctacggtat
     5761 aactgatggt gccatcggca atgccgccgt atttttttgc aaatccgaca gagcttctga
     5821 tattatccat gtcgttcagg gcgtcgaaaa tgcgcaaaat gtcaatgccg gttgacatgg
     5881 catttttgat aaatccttcg attacatgtt caggataggg attgtatcca aaaaggttgc
     5941 gaccacgtga taaggccgtc agctttgatg aggttccgat tgcgtccttg atttttgcca
     6001 atcgttccca tgggtcttcg ttcagataac gcatgatact gtcaggaaca gctccacccc
     6061 atacttccat ggcgtaaaaa ccggcttctt tgaacaatgg aagcacatga tcgacctggg
     6121 cctggttcat acgggttgca aataatgact gctgtccatc gcgcagtgtg agatccctta
     6181 tctttaattc tttcatagtc ttgactttta actgcgtaaa attacaacta ttttgagaga
     6241 attccggatt ttttaacccc tgatatgtat caacattcat tgacaatggc ttttctcttt
     6301 tgtatttttg cataaaattt tttcattatg aaatcagata ttcagattgc gcgcgaaaca
     6361 tccatcaaac cgattactga aattgcaaaa caattgggga ttaatccaga tgatctggat
     6421 ttgtatggaa agcataaagc aaaaattccg cttagttata ttaatgacga aaaaataaaa
     6481 aaatcgaaat tgattttggt aacagccatt acaccaacac ccgctggcga aggcaaaaca
     6541 accacttcta tcggattggc tatgggcttg aaccgcatag ggaaaaaaac aacggtggtt
     6601 cttcgcgagc catctctggg acccgttttt ggagtaaagg gcggcgctgc cggcggtgga
     6661 tatgctcagg tgattccgat ggaagatatt aacctgcatt ttactggcga tatggcagct
     6721 gttgaaaaag caaacaacct gttgtcggcc ttgattgata ataatcttca gaatacaaaa
     6781 aacagtctga atcttgatcc gcgcaccatc agctggaaac gggtgatgga catgaacgac
     6841 cgctcgctgc gcgacattgt gattggtctt ggcggaactg ccaatggaat tccacgtgaa
     6901 accggtttta atattactgc ggcttcggaa gtgatggcaa ttctttgtat ggcctctgat
     6961 aaagacgatc ttgaaaagcg tttgggaaat atttttatcg gatttacctt cgataaaaaa
     7021 ccggtatatg ctcgtgatct gaatgctcag ggagctatgg cggtgttgct gaaggatgcc
     7081 attatgccca atctggtgca gacacttgat aatacaccgg caatcattca tggtggacct
     7141 tttgccaata ttgcacaggg tacaaattca attattgcta caaaaacggg tttgtcgctt
     7201 tcagaatatg tagttacgga agctggtttt gcgagcgatc ttggagcaga aaaatttttc
     7261 gatatcaaat gtcaaacagc cggattgatg ccgaatgcag tggtgattgt tgcaacggtt
     7321 cgggcactta aatatcatgg cggtgccaaa aaagaccagt ttgcaatacc tgatgcagcg
     7381 ctggtttcaa agggtttgcc aaaccttgaa aagcatatcg aaaacatgaa aaagttcggt
     7441 tttactccgg ttattgcact caataaattt gatactgaca gtgttgagga aattaaagta
     7501 gtggaggatt tttgcaaaac tcaggatgtg tccatggctg tgaatgattc gtgggccaga
     7561 ggagccgaag gcgcacttga attggctgaa gcggttgtta gtgaggcaaa atcatgtccg
     7621 gcttgtttca agccgctgta tgataaaaat tgggatgtgg agaagaaaat cagtaccatt
     7681 gtaacaaata tgtatggtgc caaagatgtt gaatattcgc tcaaagccag aaaagacctt
     7741 cagaatattc tttccctggg ccttgataaa ctggccattt gtatggctaa aacgcagaat
     7801 tcattgtccg atgtgccaga attgcgcggc cgcccgaaag attttactgt caatatccgg
     7861 gaaatcgaaa tttcttcagg cgccggattt gttgttccta ttgcaggatc tatcatgcgt
     7921 atgccgggtt tgcctgcagt gccttcggcc gaaaatattg gctttgatga tgacggaaat
     7981 gtgaccggac tcttttgaaa atacgagatg cgaaatacga gatacgaaat acgaaatacg
     8041 agatacgaga tacgagatac gaaatacgag atacgagata cgaaatagga gatacgaaac
     8101 acgaaatgcg aggtgcgaaa tacgaggtgc gaaatgagaa tatgagatgt gacttgtgaa
     8161 atgcgaactc gaaattctat ttcgaagttt agttttgttt tgcatgaata gtacgcatac
     8221 ttgcaacaaa tatggctacc agctcttctg cttctttttg aataatctgt ccatctttaa
     8281 tttgcgaaaa gtatttcgat cgattaatta tttttaaaca gatcaatgtc tctcgtaact
     8341 ccttcagtga tattttcatt ttatgaatga agtccttggt tgattcggct gcctgtgctt
     8401 cgccataatt cagggatgcg gatacagatg ctcgtgaaag ttgatctgca aaatattctg
     8461 caatatgaat ttgcgggaaa tcatgacaga aatctagaat tgaaacggag aatttcacca
     8521 aacgttcttc tgtctgatat ctgcttttca cgtcttctac acatgaatca attaacacgt
     8581 cagttggata ttgtttttgt ttcataattt tatattgttt cgttttctaa atatgccgtc
     8641 cctctgttca tgttttatat ttccgatttt gattttattt agaattataa attgtcacat
     8701 tatatttcgt atttctgatt ttgtatttct aatttcgtat ttctaatttc gtatttctaa
     8761 tttcgtattt cattccggct catgcgccac agaaatcaac agattttgaa attgccgcaa
     8821 gtgcagatca aatggaaatt gttttgattt aagccgtata aaaccatggg cttttgctct
     8881 ttctgtttca aacggattga tatgaatatt tccacgtaaa gtaatgtagc gttttccgta
     8941 agctttaaaa atggcttctt taaaagtcca gtacataaca gctttttcaa ggttttctgt
     9001 ttttttccac tcttcgtgtt cttctgcatc catgaatttt tgcgttacct tgagtatttt
     9061 cggatggatg ggttcaatgt cgagtccgtg catcaacttc atgttgggtg aaataagcac
     9121 ggctgaataa tcggtacaat gtgttatgct gatggaactt tgtgggttta ccagaaatgg
     9181 cttgttgtcg tcgtcgtaat atatgcgata aatatagtcg gtttttacaa gttggcgtat
     9241 cagtgctctg taacttaacc attgacgtcg tcttgtatcg tttccaaatg aagccagtgt
     9301 gtcaaattct tctttattca gaaacaacat ggatatgagc tcatcaacac tttcggtgat
     9361 gtgccacaga cccatttgca ggtcaggttt tatccattcg tgatatacaa gaggcatacg
     9421 taaaaatcaa tgcataatta cgaattcttt ttcagtaatg ccggatttga aaataaattt
     9481 cagaaaataa aatccttcag gtaaatgacc tgtttcaatc aggttttccg tgatttcttc
     9541 tgttatgaca cgcccttgta aatcacataa atacacgtgt tccggtcgtt cagaagttgc
     9601 ataacgaatg gaaatattat tatcggccgg atttggaaaa atggaaacga tttgctgaaa
     9661 accattttcc aagccagatg taggccccca gattgaatag acccagtcgg gattatccac
     9721 aaatggattg cggttgtgct gaatgccata cacatcattg tttcgttcaa tttctttggt
     9781 gcttacagag tcaagtgcat gccactgaag cagcatgttt tcagtccatg aactcagatc
     9841 tccgccactg aatgattctc cgctccacga gggcatttca tctttgtagc gtacacacat
     9901 gtaaaaataa atccgcgcaa aatcgccttt gaaactatca atgggttcga aaacagtgcc
     9961 ggtgtagccg ggaaaaccac atggacccag tttgcttcca ttctgcgatg tccatgatgc
    10021 agaactgact tcaccaaaag gaaaattgct gcgctgacca ttcactttcc catcggtggg
    10081 cactacatga aacatgtcgc tatacatggg taaagaaccg ccaaaccagc tctgtggaac
    10141 cgaatgctcc cggttgtaac aatcgccttc gctgttgtag tttccacact gatcggtaac
    10201 aaatgtgaac tcatagggtg gcgttccgcc cgggatatcg ctgtacatat cccatacttt
    10261 tccgttggac tttttgtctg taatcggata tacatcccat aaatcgtcat aaacggtaat
    10321 tgtgtatccg gtggaaatga tattgtgtaa agctgttttt aatgcgcttc cggtcagtcc
    10381 ttcagcatcg tcataatatc cggtgggtgc ttgtgcaatg gcaaaaagag gcaccaaaca
    10441 tacccatatc aaaatccatt ttttcatatt gcaaatataa catgtaaagg tacgaaattt
    10501 ttgctgtttt taatggaaat gatttggttt ttggcgtata ttttaggaaa tgtctatcaa
    10561 aaagcgccca aaactttcat aatctggaaa tccgacggtg aatttgtgca ctaacttcga
    10621 cagaattttg ctttctttcc aaacttcaag cttctcaaaa ctataaacga atctcataat
    10681 attatttttt gtttgataac ttctcaacta tcaatgcgct tccagccaat ttctgccgtc
    10741 ctttacttcc acttccaccg ggacttttag cggcaatgcg gctttcatga gtttctcgat
    10801 ttctggtttt acaatttcca attcttcttt tggaacatca aataccaatt catcgtgcac
    10861 ctgtaaaatc atttttgttt tcaggttgct ttttttcagc caatcaaaaa tatcaatcat
    10921 ggccattttg atcatgtcgg ctgaagagcc ctgaatgggc gcgttgatgg cattgcgctc
    10981 ggcaaaacct cgcacaaaac tgttggttga atgaatgtct ttcagatatc ttcgccggtg
    11041 tttcagtgtt tcgacatatc cattagcgcg tgcaaaaaga atagtgtcgt ccatatattt
    11101 tttgatgccg ggaaattgtt caaaataatt atctataatg tccgaagcgt cttttcggct
    11161 tattccaagc cgctctgaga gcccgaatgc agagattcca taaatgattc cgaaatttac
    11221 catttttgca tgacgacgtt gctcgggttt tacatcattc aaggcaactc catacacttt
    11281 ggctgctgtc gctgtatgaa tatcaatgcc ttgtctgaat gcatcgacca tatgctcatc
    11341 acctgaaata gacgcaatga tgcgtaactc aatctggctg tagtcggccg aaatcagtac
    11401 atgattttca tctcggggga caaaagcttt gcgaatttcc tgtccgagtt cggtgcggat
    11461 cggtatgttt tgcaaattcg ggtttgttga actgaggcga cctgtggatg ttactgcctg
    11521 attatagctg gtgtgaactc tgcctgtttc cggatgaatc agttcgggca atgcatcaac
    11581 ataggtgttt tgcagtttgc tcaaaccacg gtattcaagc acttttgcca caatcggatg
    11641 tcgtgaaata tatttttgca acacctcttc cgatgtggaa tattgttttg tcgccgtctt
    11701 ttttggcttt tcagcaattt gtaatttatc gaaaagaatt tcacccaatt gtttcggact
    11761 ggcaatgtta aagggctgtc cggccagttg atggatgtcg gattccaatt gtaatatttc
    11821 ttttccaagt aattgcgaga attctttcaa tgattgctga tctattcgaa cgccgctgcg
    11881 ttccatatct tcgagcactt caatcaaagg ggtttcaatt tcataaaaca gctctgtcat
    11941 gccggaaata tccagctctg gttcaaaaat tttccagagt ttcagcgtaa tgtcagcatc
    12001 ttcacatgca tagtctttga gtttttctgc agtcacatcg aaaattgaaa ttttttgttt
    12061 gccgcttccc agcaattctt cgtaagtgat ggttttgtag ttgaggtagt tttcgctcag
    12121 gtaatcgaga ttgtgacgaa gttcgggctg gagcaggtag tgtgccagca tggtgtcggc
    12181 cagtttgcct tttatgtgaa tgccgtgtgt tcgcattacg gcaatatcat atttcagatt
    12241 ttgtccgatc ttgatgattt tttcattttc aaatattggt tttaacattt ccaatacgga
    12301 tttgcagaag tcctcgtctt tcagataaac aaaaaacgct tttccgggtt cagtgcaaaa
    12361 cgaaattccg gccagtgttg ctaccaatgt atccaatgaa tctgtttcgg tgtcgaagca
    12421 gattagcttt tcttttttta gttccgaaac aagtttttcg agatcatctg tgttgtcgat
    12481 gagtttgtat tcatgttcat tgctgttgat gttgctgatg atggaatcca gtccggctct
    12541 ggcttctaca aagtcctgat caaataaact agattgacct tgagcaacag ccggcgtttt
    12601 ttcggcaatt atgcccatag catcccacca gcgtttcgaa aatgttttga attccagctc
    12661 ttccataaat ttctgcaatg gcgcaaatgg cgcaactttc cattcaagcc gcgaaggcat
    12721 gaattggata gggacatcgg taatgatggt ggcaagttgt tttgagataa gagcggattc
    12781 tttaccggcc agcacctttt gtttcatggt gggatttttt atttcatcgg catgtgccag
    12841 aagattttct acactgccgt attcgctgat cagttttttt gcacccactt cgccgatgcc
    12901 aaaaatacct ggaatattgt cggccgcatc tcccatgatt gcaaggatgt caatcacttg
    12961 ttcaggacgt tcaattccat agcggtcgca tacttcgcgt actccccaaa cttcggcagc
    13021 ggcgccgaat ttggcgggtt tatacatgaa aatattttcg ctcaccaact ggccgaaatc
    13081 cttgtcgggg gtcatcatat aggtttgata accgcgggtt tctgcttctt tggccagtgt
    13141 tccgattaca tcgtcggctt cgtatccgtc aacttccaga atgggaatat ccatgagttg
    13201 caacatctct ttaatgtaag gaagtgagat ggacaaatca tctggcatgg gttcgcggtt
    13261 ggctttgtaa aatgcaaatt catcatgcct tgctgtgggt gcaatggtgt caaacgctac
    13321 tcccagcaaa tgtggttttt ctttattaat gagatcgagt atggtgttgc caaaacccag
    13381 aatggcactg gtgttcagcc cttttgaatt acgtacggga tttttaatca tcgcgtaata
    13441 agatctgtat gccaatgcca tggcgtcgag cagaaataat accggtttct tttcttccat
    13501 tgaaaaagat gtttttgtaa aagtagttat ttttttagta gtcagtcccg cgtaatcagt
    13561 cccatgcatt aatacattat tcgttcattt tgtttattta ttattctttt ataaatgttt
    13621 tttcagccct ttaacccttt aaccatttag ccctttaacc atttaaccat ttagcccttt
    13681 aaccatttag ccctttaatc gtttagccct ttaactcttt agccatttaa ccatttatca
    13741 gtttaatgaa tcaacaacaa tcatttccgg tctttacaat tccctaacat tttctttttc
    13801 gataattgtt cattcgttca atttcagata ataaagcgga tgtttctttg cagcataaac
    13861 caaatgaaaa aagtatgaaa aaagttttga ctgtagtgtt gggtttgatc atgtcagctg
    13921 gaatatttgc acagcatagt atcgaaaacc ctttttttga tcaggtaacg tatcgtggtg
    13981 cattcgatgc cactacttta tggacggatg gatggaccaa ctgggatgcc gaaaacacaa
    14041 cgtatccggc aacaacagtt acaaaatccg gtgagattac aagcaacgaa acctggactg
    14101 cttcggatgt gtataaaatt gaagggtttt tatatgtgcg cagtggtgca acgttgacta
    14161 tcgaagccgg cactgtgatt cgtggtgata aagccagcaa gggcactttg attgtggaac
    14221 gaggtgcaaa actgaatgcg gttggaacat ccgcaaatcc aattgttttc acatcaaacg
    14281 aagcggcggg ctcgcgtgac tatggcgatt ggggcggcat tgtcctttgc ggaaaagcgg
    14341 ccatcaatgt gcctggcggt gaagctcaga ttgaaggtgg accgacttcg tattatggcg
    14401 gtggcacttc tcctgacaat gctgacaaca gcggtacgct gagttatatc agaatagaat
    14461 ttcctggaat tccttttgta acggataagg aaatcaatgg tcttactctt ggtggtgttg
    14521 gtagcgccac aaccatcgat catatacaga tttaccgttg tggtgacgat gcttttgaat
    14581 ggtttggcgg aactgtaaat gccaaatatc tggtttcttc attcacctgg gatgatgatt
    14641 ttgataccga ttatggcttc tccggcatga ttcagtatgc tgttgctttg cgcgattcat
    14701 ccattgctga tccgggttcg ggatcaaacg gttttgaaag tgataatgat ggctcgggtt
    14761 cggccaatac tccggtaacc aatccgattt tttcgaatat ttccatgttt ggaccaaaag
    14821 tactttcaac cacaagcatc aataccaatt ataaaagagc gatgcatttg cgccgaaatt
    14881 cgcagatcga tatttacaat tccttttttg ccggatataa agatggactt tttgttgatg
    14941 gttcagcggc ccggcagaat ctggctgaag gcgaattgtc tctttttggt gtaaccctgg
    15001 cttattgctc tggaaattat tttccggaag acaccgtgaa tgttgtgcgt gattattact
    15061 taacatcctg gagaatgaat gatacattga ccgatcatgc tttgctgcag attgccgatc
    15121 ctttcaatta tggcaaccct gattttcgcc ctgtgactgg tagtccggct ttgaacggct
    15181 cggttttcag tggcatcatt gaccgcgaaa gcaacaatgt tgatgagttg attatttatc
    15241 caaatcctgc aacaaacgtg cttaatgttg ctttttctct ccgtgacgac gaaactgttg
    15301 ttgttgagat tatggatatg agcggtagaa tttgcagcag ggttgttgaa agcagggaaa
    15361 tgggttcgaa tgttgtcgga atcgatgttc atcaactgga aaccggtgtt tattccgtta
    15421 ggataaaagc cggttcatct attgccacca tgcgcttttt caaataacat acatctgaac
    15481 ctctcatgaa tcagctgggc ataactgaaa agttatgctc atgctgcttt gtgtaatgtg
    15541 aatttattat tagtaacatt ggttttttac taacaatttc ctgacagaaa aaagatgcat
    15601 taataaagca gttttaatta cagggtaata tttgaaatgg aaatttgcag tacaaatcag
    15661 ttgtatgaaa aagttgtttt tattttttat tatcagtttt tcgttttcgt ttttgttctc
    15721 acaaacgagc tttcagagcg aagttccggt aagacagggt acaatggatg taaaaggccg
    15781 tgttgttgat gcaaaaacaa atgagccggt tatcggtgcc aatgttgtgc tgaagggaac
    15841 aacccttggc gcttcaactg accttgacgg aaactttgtt ttgccggcca ttcccaaagg
    15901 caatcttacg gttactgttt cttatgtttc ttataaacca tttgaacata ctttttatat
    15961 ttcaggcgaa aaaaatcttt ttcttgatat taaactcgaa gaaaatataa cgcagattca
    16021 aacagtaacg gttgtctcca atcgcgttac cggaactgaa gtctctatga tgcagaccat
    16081 ccggcaaaac aacctggtgg tgaatggtgt tccttcgcag atgataaaaa aaacacagga
    16141 ccgcgatgcc gccgaggtgg taaaacgtat tccgggtatc acagtacagg acggacgttt
    16201 tgtgattatt cgcggattga atgagcgata caacagtgtc tggctgaatg atcttcctgc
    16261 tcccagtgcc gaagccgatg tgcgtgcttt ttctttcgat ctgattcctg cttcgatgat
    16321 tgataatgtt gttgtgtata aaacattttc tcctgagttg ccgggtgact tcgccggggg
    16381 gctggtgcag atttcaacgg taagcaatcc ggagaaaaat catttgcaga tttcatactc
    16441 aaatggctat gctgccggct ccacttttca gcaacatctt ttgtatcctg ttgaaggcag
    16501 cggaatcaca aacgccactt cttcgctcaa acttccgcaa ttgtttcctg aaaatgtatc
    16561 aaccatccct tcaaccgaag ccggaaagga cttgaaaact ttttggggtc agacaatgaa
    16621 taaaatctgg acaccgcatt caatcacagc tccaaccgat cagcgttttt ctgtttcagg
    16681 tgcctggagt aaaaaatggg acaatgccag attggtgaat ttttcacaga ttaattacag
    16741 ctcgactttt cagcaggaca gcatttttcg tgcatcatac aatgcatatg atgaaattgg
    16801 tcaaaagccc gatacaaatt attatttcaa tgatgttcaa tatgtcagtt ctgttaaagc
    16861 aggagctatg atgaactggt cgctgattaa gggaaaatca acatatgaat tcagaaatct
    16921 cttgaattat caatcttcaa atcgcgttac tgagcgcgaa gggcgtgatt tttacggagg
    16981 aatttatatt cgtggtcttg agatggccgt taaacaacgt catttgtatt ctggccagct
    17041 cagcggaaca cactcttttg gaaaaaagga atcaaagata aaatggtttg ccgggtattc
    17101 ggtttcatca cagcaaatgc ctgatacaaa acgtttgacc tgggtgctga acgatcagga
    17161 ggaaagcccg tattgtggtg agtatggatt gaatttttct ttcacagcaa attccaatct
    17221 ttccggaaga atttttcatg atatgaatga acagattgta aatgctggtt cagattttaa
    17281 ttcatatatt tctgcaggaa gcagtaaaat tgcatataaa accgggtttt tctacgaaaa
    17341 ccgtaaccgc ctttttaatg caagaaacct ggggtataaa attgcaaaga catctttatt
    17401 caattgggac cttccttatc agagtgtgga aaatgttttt ggcaatgaaa atatcaatgc
    17461 tgctgatgga attatgcttg atgaacaaac aagtccatcg gattcataca catcgcattc
    17521 ggaattgctt gcaggatatt ttaccttaac gcttcctgtg ggttcaagaa ttaaaattca
    17581 tacaggcata agagccgaac acagcagggt gtcacttcat tcatacactg ccgatgcaac
    17641 aaatcctcct tcaactgaag atgaaattca ttacttatca gacactgttt tgtttttacc
    17701 ggccattaat tttagttttt caatgaccga aaacagtttg ttgcgattgg cttacggaca
    17761 aacgctcaat cggccggaat accgcgaaat tgctcccatg tcgttttatg attatgaaat
    17821 gaaagcggta attacaggca atgacagtct gacttttgca agcattcata attttgattt
    17881 ccgctatgaa tactatccga atgcttacag catggtcagt atcggtgctt ttcataaaca
    17941 gttcatcaat gctatcgagt atcagattat tcctaccggt agtggattgc agtattcatt
    18001 tcagaatacg cccgaagcca gtgtgagtgg gttggaagct gaaatgcgtc tgtcggctgc
    18061 ttcgttgaaa aaatttgatt atctgaaaaa tttcactttg atttttaatg ctgcttatat
    18121 gaaaagccgc atctgttttg aagaaaatag tctcgaggaa gatcgcgccc tgcaaggtca
    18181 gtctcctttt attctcaatg catccttgtt ttatcagaat gattcgcttg gtttgaatgc
    18241 cggcttgatg tttaacatag ttggcaaacg aatcagtttt gttggtgatc cgtttaccgg
    18301 caacccacat atttacgaga tgccggccag taagctcgat cttagcataa gtaaaaacat
    18361 cggaaagaaa atcgaagtaa aagcgcaggt gagaaatttg ttaaacagtg cctctcgata
    18421 cgctcagaat attgaaacag agtcaggtac cattgaacag actacaatgc aatacaatcc
    18481 gggaagatat tatcttttgg gtcttgtttg gaaattgtaa tttaaaacat cgataaaacg
    18541 acaaattgtg ttgccgaaaa ttaattttca acattcagaa attcgacgct tatatgtcgt
    18601 atttttgaaa catggaagag aataaggata agaaaaatac caagcgtagt gtaaaacctg
    18661 caccattaaa ggcctcagta ttagatcacg ggcgggtacc cccacaggcg gtcgaacttg
    18721 aagaagtcat tctcggtgca ttgatgcttg aacagaatgc tctgaatact gttattgata
    18781 ttattcaact tgattcattt tataaggaag ctcatcgtta tatttttgag gccattcagt
    18841 ctttgtttgc ccgaagttct cccatcgata tgcttactgt tatcgaagag ttgaaaaaac
    18901 aaggaaagct tgaacttgtt gggggcgctt attatattac atacctgacc aatcgtgtgg
    18961 tttcaagtgc caatattgaa tatcatgccc gcattgttgc acagaaatat attcagcgcc
    19021 gtttaattga aatttccacc gaaattatca atgattcgtt tgaagaaagt accgacgtgt
    19081 tcgatttgct cgatagtgcc gaagacaaac tttttaaaat caatgaagag aatttaagac
    19141 gcaaaaccaa cggcatggct cagttgctgc gctcagccag agaaatggtt gaatcggctt
    19201 cgaaaaacga aaactctctt tccggtgtgc caagcggttt taaatcgctc gatgctgtta
    19261 cagccggctg gcagcgctct gatctgatta ttattgctgc ccgcccgggt atgggtaaaa
    19321 ccgcatttgt gctttctatg gcacgcaata tggccatgat caacgaagtg ccggtggctt
    19381 tgttttcact tgaaatgagc gccattcagc ttgtgatgcg tttgatcgct tccgaagcac
    19441 gcatcagcag cgagcgtttg cgaaccggca agcttacagc gcaggaatgg aaaagtctga
    19501 atgataacat ggacaatctg tccaaagcaa aattatatct cgacgataca ccggcactct
    19561 ccgtatttga actgcgcgcc aaggcccgcc gactgaaaca gcaatacgat attcagtgtg
    19621 tcattgttga ctacctgcag ctcatgactt cgaaggtgga gaaaaatgcc aaccgtgaag
    19681 ttgaaattgc aaccatatca agatcattga aagcactggc aaaagagttg aatattccag
    19741 tcatttgctt gtcgcagctt agtcgtgaag ttgaaaaacg tcctggcagc aaacgtccgc
    19801 agttgtctga ccttcgcgaa tccggtgcca ttgagcagga tgccgatatc gttatgttta
    19861 tttaccgtcc tgaatattat tttaaggaag atgataaaaa tacggatatc gaaaccattc
    19921 gcaacaaagc tgaattgctg attgaaaagc atcgtaacgg tccgcttaca accatcaatc
    19981 tgaaatttct cggagcttat gcacgttttt atgaagaaaa tgacgaggat ttttctcatg
    20041 ccatccgtat tctttcagaa agtggtcaaa ccagctccgg aagctctttt acggttcaat
    20101 caaaaatgaa taatgatcat tccggtgatg atttttccat ccgtaaaagt gatctctgat
    20161 ttttgcatga ttattgaaaa gcttttgttc caatgaaaac ccgatatttt cttgttctgt
    20221 ttgttttgat ttttttaccg cgtttagcgg atgctacaca tttgcttata ccaatggatc
    20281 agtcgcagaa aaatcattta aaagcttatg gtatcaccta ttggattctt gctaataatg
    20341 aagaagctga atggttgctc aattacagag gcggtagttt tatgtgtggt ttttcaaaaa
    20401 ccattcagga cgaatgcaat atcaggggcg tttcggtgga ggtgatttca gatgcacaag
    20461 ccgcttcaat tcgtgcagaa attgccgatc cgtctgttaa tatggatatt attcaactta
    20521 ccaaagctcc gaaagtggct gtttatgcac ctaaaacaaa cctgccatgg gatgatgcgg
    20581 taacattggt gctaacatat tctgaaattc catatgaaca gatttatgac gaagaagttt
    20641 tgagcggcga tttgcccaaa tatgactggt tgcatttgca tcatgaggat tttaccggac
    20701 aatatggaaa attctgggct cattatcaga atacagattg gtataaacag gatgtggcag
    20761 aatcggaaaa atcggccaaa gctcttggtt ttactaaggt gtcgcaaatg aaactggcag
    20821 tggccaaaaa aattcgtgat tttgtagctg ggggaggtta tctcttttcc atgtgcagtg
    20881 ctccggatag ctatgatatt gcgctggcag ccgaaggtgt tgatatctgt gcttcgcagt
    20941 tcgacggcga ccccatggat ccgcaggcac aaagcaaact cgacttttct aaaacctttg
    21001 cattcgaaaa tttcaaaatc agcaccaatc catacgaata cgaggtctca aatattgatg
    21061 tgtctttgtc gcgcagagcc atgaatccga gagatgatta ttttgcgctg tttgaatttt
    21121 cggctaaatg ggatccggtg ccaaccatgc tatgtcaaaa ccatgaacgt ataattaaag
    21181 gttttatggg tcaaacaacg gcttttaatt cggccacact gaaatcgtcg gtactggtga
    21241 tgggtgaatt caaggccatt ggcgaagcac gttatattca tggcgaatat gccaaaggta
    21301 cctggacttt tctgggtgga cacgatcctg aggattacca gcattttgtg tatgatccac
    21361 caaccgatct taatcttttt cccaattcac ccggttaccg tttgatttta aataatgttt
    21421 tattccctgc tgccaaaaag aaactgcaga aaacttaagg cggttttgtt tgttttttgg
    21481 cgattgtcag aattttccct gcggttgttt ttttgttaaa aagagcgttt tcaaagttaa
    21541 tttttgattg caggacaaag ttgcgtgcaa cttttggatt ttttgaattg tcatattgca
    21601 ggatttttat acatttgaaa aaaaaagcac catgagaatt tttacctttt tgattttgtt
    21661 gatgatggtt ttgggctctt ttgcacagtt gagcatcgag ggttcaccca aaagttttaa
    21721 gtcgttcgga acgcattcgg ttgttccttt tgttgatttg cagaaacccg atatgactgt
    21781 tgtgaatgca caggatgagg ctgcagatat ggccggaaaa gcttaccgta atggaatttt
    21841 gctgcaaaca gaaattactc ccgaaaccag tggtgtttgg gattatctgc ccgatggttc
    21901 tgctatctgg cgcgtaggtg tgaaatcaga caatgccatt gctcttggtt tgtatttcaa
    21961 cgattggtat ctcacccctg gcattgaggt ctatgtttat actcccgata aatcttttgt
    22021 tatcggagca tttactgaac ttaataatat tgatctgaga accatggcaa ctcaggaaat
    22081 tccgggtgat gaagccatcc tcgaagtttt tgttccggat cgtgatcaga aatttgacct
    22141 tgccctcaat caggttgctt atttttacag aggattgaat attgtaaaaa atgtaaaagc
    22201 ttcggatgat tgtgaggtga atgttatctg cagtgaaggc gataactggc gcgaccagat
    22261 cagaggcgtt gctaaaataa gcatgattct ttctgatgga gcttacatgt gttcgggttc
    22321 gttggtaaat aatattgctc aggattgtac gccgttcttt ctgacagctg atcattgttc
    22381 ggtttctcag tcaggagtgg ttgttactgc tactcagctt tcgcaatgga ctttccgctt
    22441 caattatcag gcaacaacat gtacaggatc aacttcaggt acatcaaaag tttataccgg
    22501 agcgacctat aaagcatctg atacttatgg agaaacaagt accggttccg attttttcct
    22561 ttgtcagctg aacaatacac ccgaagccac ctataatcca tattacaatg gatggtcata
    22621 ccttaatact gccggaacca gtggcgtaag tattcatcat ccggcgggcg atatcaagaa
    22681 aatcagtact tatactgcaa ctctgattaa ttatacaaca cattggggtg taacctgggt
    22741 tacaacgacc aatggtcacg gagttacaga aggtggttct tctggctctc ccatttttga
    22801 tcagaataaa cgagttgtag gtacactcac cggtggtgcc tcggcttgcg aagttaatgg
    22861 agccggttcc ggaacaggtc ccgatgaaat ggatgtgtat ggtaaaatga gtaaacattg
    22921 gacgggtgtt ggaactactt cagataagaa tgtaaaatat tggcttgatc ccaataattt
    22981 gtcgggtgga acacttgctg gtcggaatta ttgtgttgct ggcctcgatg atgttgctta
    23041 tatccgcgcc aatattcatt tgtatcccaa cccggcaacc gattatctga gcattgattt
    23101 ttcggattac aatgtagaaa acggaacaat cgtcgtaact gatatgcttg ggaaaaccgt
    23161 tacagcaccg gtgaatggtg actttacagg aagtgaaagc attgatgtgc gagaacttga
    23221 accaggtatg tattttctga tgattcagtc acaaagtacc agtgtttcgc tgccttttgt
    23281 agttgaataa tcagaagtat taaagtatta gttttgttgt gcgaagattt ttcttcgcac
    23341 aacttatttc taagctatgg cattttattc tctcagcgaa attattgacc tgctccgcaa
    23401 acgcagtctg gtggccgaag ttggcgaaga ggtcagcccg attcttgaag catctgaaat
    23461 tgcagatcgg tttgcctcag acgaaaagtc gggcaggcca ttgttgtttt tgcagaatgg
    23521 gacagcattt ccgctgctca tgaatttgtt tgccaataaa gaaattacag atgaattatt
    23581 aaaccttaca ggtacagaag gatatttttc gcgtctgcag aatctgttgt ctatgatgga
    23641 ctcaaagccg cgattgtcgc gcaaaagcat ccggtcatta atgggtctcc gaaaaacagc
    23701 tccaaaaaaa ttgcatcaca aaggcgcatg catggctgtt gtacagccgg cacccgattt
    23761 gaatcttctg ccggttattc aatgctggcc gtacgatggt ggccgttttc tgacactccc
    23821 tatggtgcat acgattgatc ctgacacagg aaaacagaac actggaatgt acagaatgca
    23881 ggtattcgac ggccagtcaa cgggtatgca ctggcatcgt cacaagggtg gggctgctca
    23941 ttttgaggcg tggaagaaaa aagggaagcg gatgcctgta actgttactc ttggtggaga
    24001 tccggtgctg acttacgctg caactgcccc gctgcccgaa ggcatcagcg aatatatgct
    24061 cgctggtttt ttgaaaaaca agagggttcc tttggtgaaa agtcacaaca gcgatatctg
    24121 gattccggaa tgcagcgatt ttgtaataga aggctatatc gatccttcag agccattgcg
    24181 aaccgaaggt cctttcggcg atcatacagg tttttattca ctgcccgatg cgtatccggt
    24241 atttcacatc actcgtattt cacaccgcaa aaaggcagtg tatcctgcta cattggtggg
    24301 cgtgccacca aaagaagatc aatttcttgg acatgcaact tcgcagattt tctttccatt
    24361 gattcagaaa atatttgcac ccgagattct ggatttgtac ttgcctgtgg agggtggatt
    24421 tcataacctt gcgctggtga aaatccggaa aaaatttccc ggtcaggccg taaaagtgat
    24481 gcacgctttg tggggagccg gacagatgat gtttaccaaa tgcatcatgg ttttcagcga
    24541 agaggttgac atccggaacc cgcatgcagt gcttgatgcc attgataaga attttagtcc
    24601 tgtgcaggat ttacatatgt cagccggccc gtacgatgtg ctcgatcacg cagctcaatc
    24661 cttttctcat ggcggaaaag cgggatttga ttgtacgttg aaaacagaag aaagaaagaa
    24721 tagtccgacc ggtaatactg aagataagtc agattccatg cggcttttcg gcaagtcgat
    24781 acatgtgata ttttcagatc acgcggtggc cgaaagtgga aatctttttt tgaatttggc
    24841 tggcggagct gctcccgtgc gaaaaggaat ttatataatc actgacactt gcatgaaaga
    24901 agctccggat aatattctgc tgtggtatgt gctggcgtct attgatccgg cccgcgactt
    24961 ttcgctgatc cgcagtctgc ctgccgaagg cgttctggtg atcaatgcct gcgtaaacgg
    25021 aaaacgcgca gtgcctggcg gacgctggcc tgcagccacc gttatgtctg atgaggttgt
    25081 cagccttgtg aatgaaaaat gggaatccta cgaagtcgga gatttcattg agagtccttc
    25141 aacccggctg tccgagctga aaagtgattc gtatttgaat aggtagcaca aaagtttatt
    25201 attgaaaaca atggttttta tttgcactat tggtttttga taatcgattt gatcggattc
    25261 aaaaaccgtt caatcaatct cagatcgtct gtaattattt ccgctgatcc tgacatctcc
    25321 tgggaaaaat tcagaatctg gttgtaattg gtgcgcaaac catcgggaaa tgcaacttca
    25381 acaatgtagt ttgattctgt tgtaaccaat gaaatggatt tcaccatccc ttttaccatc
    25441 ccatattcca tgtgaggaaa attatcaaac tttatgttga tttgctggcc ttgcttcact
    25501 ttgccggctc cctgcatggg taatacaatg gttccgacaa gctcactttt tttctcagga
    25561 accaccgtca tgacaacatc tcccgaaact acattctgat tgacttctct ggtatttgta
    25621 aatgtaactt taccatcaat cggagattga atcagataag tgtttttcca gatattgatt
    25681 gaattggata atgttttctg agattgctga atggattgaa tgtactgctt actttcatcg
    25741 gcctgctgca attgcatctc cacaacctgc tgttcaatct gtgccttctg aatttgcgta
    25801 ctggacaaat ttgtccgtga tgacatatat gattgcttcc tctgaattaa cgaagctctt
    25861 gacttatcga attctgcagc actcagtact ttctgtgaaa acaaaatgga atcgcgccga
    25921 aattcattct gagcaatctg cagatcttct ccctgtaagt tgctttgatc cgaaagccgc
    25981 ctgtaatact gatctgtcaa tgatagttgc ctttcaagag cagtgatttt tttcgacaag
    26041 agatttacac ttttatacaa taaataatcg gacacagact tttgaaacat tgtaaaatca
    26101 gattgcagat cacccagcaa taaagcaggt actaaggcaa tattctcaag tgcactgtca
    26161 ttattcacat caaatgtcag caataaacta tcaagcaaaa tcacatcatt gtactctgca
    26221 ttgttttcaa tgacggcaag aatgtcgcct ttgtgaacca tttgattatt cgaaacaaat
    26281 aaatgagtga tttttccact ggtctttgca ataagcgatg caggtacctg agtagtgctg
    26341 acttttattg cagcagttac tttatccggg tatttaataa accaggaacc agctagtaaa
    26401 agaatgatga caattccgat gagtgtaacg ccatttctga ttattccgga tggcggtttg
    26461 ctcagaatat catgtacttc ttcggtgtat atttcgggca acttttcttc catactgtaa
    26521 acgattaaaa agttaaacga ttaaactact atactattaa atgattaaac gaataaacta
    26581 ttaaacaaga aatatattgg gctgatattt tagaatacat cgcttaaaat ttaagtcttt
    26641 catttgtgaa ttttatcagc tgatgaattt caactggcaa tgcttcaaga aacttgcgta
    26701 ttttgaatag ctgatcctct gtaatcaatt ttctgtcaaa cgctttttgt gcccaggcgc
    26761 gggcttcgta aagtgatcca taagcatatc tgaaaaatac aattctggac ctttttccat
    26821 aacgtccaaa cccctctgca atattggctg ccacactatc aacacaacgg acaaactgtt
    26881 ttccaatagt gtctttggca aaatattccc attcatttac aatgtcccaa acaagagtcc
    26941 ccatctccat cgccagcctg tacgaagaca agtcgttcaa atccagatat tttttttctt
    27001 cagccatttt ccattttttt atagatcaat cccatcaaaa ataaacaatt tccacatgtc
    27061 atttagataa aacaaaactt ttcccttctt cattatttca tttagccatt tgagtcattt
    27121 aatcatttag tcatttgagt catttattca tttattcatt tagccattta tctatttagt
    27181 cattaaacta tttaatcatt taaccctttg attcatttag ttatttaaca atttagccat
    27241 ttgagtcatt tgagtcattt attcatttag tcatttattc atttagccat ttagtcattt
    27301 agtcatttag ccatttgagt tatttagcca tttattcatt tagccattca tcagttcccc
    27361 agctccaatt gatttttcac cagctcataa tacgaaccac gcttctcagt aagttctgca
    27421 tgattgcctc tttcaataat tttccctttc tcaagaacaa ctatctgatc cgcatttttc
    27481 actgtgctta aacgatgagc aaccaccacc acggtctttc ctttaaaaaa ttcattcaga
    27541 ttatccataa taaccctctc attattggca tccaacgaat ttgtggcttc atccagaaaa
    27601 ataaactgcg gatctttata taccgcacgt gcaatcagca tccgctgctt ttgtccctga
    27661 ctgatgccac ttccctcctg acctatttta gtgttgtaac ccagcggcaa tccttcaatg
    27721 aattcacgga tattggctac tttaacggca tgcagcattt ttcgccgatc gatatcatca
    27781 tcgcccggag caatattctc tgcaatggtt tcgctgaaaa tatatccatc ctgcatcaca
    27841 acgccgcatt gcgagcgcca gaacgaaccg ttcacatgct ctatcgacaa atcaccaact
    27901 ttgatttcac cttgttcagg tttataaaat ccaagcaaca gttttatcag cgttgtttta
    27961 ccactgccgc ttgtaccaac aatagcagta atcttccctt ccggaatatc cagatcaata
    28021 ttttcaagca ctgacggact gttcggccct tcatagcgaa aggtgatatt atttacatgc
    28081 agacttttat tctccggtaa aatggcaaca cgattcatct cttcgggctc ttcatcgggc
    28141 ttgttgtgta tttcgcccaa acgctccaga ctgatacgcg catcctgcaa cgactgaata
    28201 aatccaatca gctgattgat cggagagctg atctggccaa tgatgtacga aacggccatc
    28261 atcataccca atgtcatttg tccgttgact accgcacggg cagccagaaa cgaaacaaat
    28321 atgttggtgg cctgcgccag aaacaatgag cccacctgct gatattgtcc aagggccaga
    28381 cctttgatgg aaaccttaaa ctgcgctgcc tgcaggcgtt cccaacgcca gcgtttctgc
    28441 ttttcgcagt tgttcagttt tatttcctgc ataccggtga tgatctgaaa caaagtgctc
    28501 tgattatcgg ctgccagtgc aaaacgcttg aaatcgagct ctcgtcgttt tttcataaac
    28561 aacagaatcc atgcaatata catcgcattg ccaaatagaa aaattccaag tattttcaaa
    28621 tcataccacg caagcacagc actaaatata acaagattta ccatgctgaa taacgttgaa
    28681 agcgaggatc cggtcaggaa attctgaata cgcgtatgat cgccgatccg ctgcatgatg
    28741 tccccgatca gcttactgtc gaaaaatccg atgggcagtt tcattaactt gattaaaaaa
    28801 tcggaaatca acgaaatgtt tattcttgta ctgatgtgca gcaatatcca gttccgaaga
    28861 aaatcaaccg acatctgcgt ggcagctaat accaattgcg aaatgagaat taaagtaatg
    28921 aacgccagat cctgattgcc aataccaaca tcaaccacac tctgcgtcag aaaaggcaga
    28981 atgagctgca acaggctccc cagcaacatt ccgagaaaaa gctgaacaat gtatttttta
    29041 tgcggtgtca gatatttgaa taaaaacgaa aacttctgcc ggtcgctttt ttcaccttta
    29101 aattcataaa aatccggagt ggtttcaagc aacaaggcaa tgcctttgtt ctcatcatcg
    29161 ctcttggtgc tgatccatcc tttttcaaat tcttccttgg tgtatttgat taatcctgat
    29221 gcaggatcag caacaaaaac agccttgtct gtaatgtcat acaccaccac aaaatgattc
    29281 tgcctccaat gcacaatcgc aggcaatggc gcttcctcct tcagttgttc aaacgaaact
    29341 tttacgccca gcgttttaaa cccgatggat tctgccgcat cactgatgcc cagcatactc
    29401 actccatgcc gcgtaatgct gcttttctcg cgcagatatg ccagcgaata gtttcgccca
    29461 tagtgttttg ccaccatgcg aagacaagtt ggtccgcaat cgcgggaatc gggttgcaaa
    29521 taaatgggaa ctctcatttt tgtgaaaaag caagtattaa ttttcaataa acgaaaaaac
    29581 aatagatatt ttgtttattg gagaatcggg ttttaccaca gatggctttt gagaccaatg
    29641 aatgacatac atcactctat catcgctaaa tccatcccat tcaatatatg acacaattcc
    29701 gttgcacatt gctgtaatct gatctgcatt agcaaggtct gattcatcaa actgtgtata
    29761 gcaatgaaac acaacctttg ccttttcatt tttcaataat tcattcagca cttggctaaa
    29821 aaacaggaat gatcttgttt caggcttgta ttcgcgtgaa agaaacagtc ggccacgtgt
    29881 atcaatctcc cgatccggaa tgatccagtt gcatgttgta tccaccaatg ggtctgttat
    29941 ttgaatataa gcgatgcgag agcaatcagt atatttataa ttaagtttta cacgcgaatg
    30001 atgagaaata ctatccattc gaatacaaaa acaattcata gggttgctca cttgctttcg
    30061 ttccagatag gactgaacaa acaatgcacg cttataataa cgatgaaacc attgaggttc
    30121 agatacagaa actttgtaag cagaatccgg taccaagttt gcaacggcga aaatatttaa
    30181 tactgcatgg tcaagactga caagttcaag cgcaagtgaa tcaagaattg aacaggtaaa
    30241 agacaagtct gatcgttcaa ttgcatcaaa aaaacacgtt gtataaatcg tatcactaat
    30301 actaatctgc gatattgact gatgacgaaa gcatttgcat tgatcaaaat tctgcgcggc
    30361 aagcgagctt gaacaaatta tgatgatgaa aagtaacact tttgatttca tactgatatg
    30421 ttaattcgaa atcattttta gatattcttc tgcagttgta tcaaagccca ttttccgtct
    30481 tcgcttatca acagactttg gcttacggac aggttgaaat attagagcac cataactgtc
    30541 acgcattaac tgtgtagcaa atatttgctt tttgttgcga tatactttat aacgatcaat
    30601 ataataagca taatcgagcg atgtaatctc gcctcgtcta tatagtttcc taatgtcctt
    30661 ttttgtatta acaaaattag ctttactaaa atgctgaaca caaagcatat attggttcat
    30721 ccccgtttta ccaactttat tttgcgtcgg gagtccttta cttttaatag tttgctgtag
    30781 cataaaaaaa ttcaaagaat caagtaatga ctgagcatca agaaaagttt tcattttttc
    30841 ctgatctgaa aaataaaaac ggtataaaga acgatagcga cttgtattca agtagcaatc
    30901 cgacaaaata cacatataat caaaatctgc gttggaacat ctgtttatgg catctgctat
    30961 aaacggcttt tgcagatcag taaacacttt ttcaccaagc attgtgcgta ttttttcatc
    31021 tctaaaaata aataaaatat ctacttccgg gctcactgtt cccgcaactc gcagaaaacc
    31081 taccgaatcg cccaaagatg cgtaactatt taataaataa tagtacagaa acccatctat
    31141 tgaagaattc ttcaacaact cttcggcttc gctcacacac gtagaataat cagctttgta
    31201 aaaagcatca atatattccg gaatagagtt gtcaatcacc ataatggtat cattaattac
    31261 aatcgtatct gtttgacaga ataaagtaat gcttgtaaaa aaaagaaaaa ttgagaagac
    31321 aaaaaggttc gctttcattt ccatttggtt gttttagggt tatatgaaca attcaagggc
    31381 ttttcaaaaa aaccatcttc aacagtaacg gctctacaat cgtgacaaaa atagcgaaac
    31441 tcgcaaccac agcacacgga aatatcatcc ttcttgatgt gccaccaatc ctgaaacttt
    31501 tgtaaagtaa ttatatcagc caaggaatct tcaaatattt tgccatgcga ttgtgtaaat
    31561 aatggacagt ttttaatgtt tccatcttca tctatcacca cctttttaaa taaacatgaa
    31621 ttaaatgatt ttgaaagaca aaaatgtgag taatctaagt ttaatagcga tggatggaaa
    31681 accccacaat cgtttttggt tagctttcct tttatgaaat attggttagg gggacctggt
    31741 atataatcac tttttttaca tccgaaataa aatatggaaa aatcaacatg ataatgatta
    31801 ttatcacaaa taagactata aacccgactt tgactatcta ttggcaagag caattcacaa
    31861 tgtaaaatat gagattctat tatttttgat atacaacgtt caagatgcaa taaactagca
    31921 ttttcagaat acattctaat gcttattact ctacaattca attctgataa ttgattaatt
    31981 gcttcagaat atatagatga gtttgtattc tcgtggataa ttataattgc attttctatt
    32041 aagttgggag aatcgtataa ttctgacaac ttcaacattt tacgactgtt tataccgctt
    32101 gtgactatcg caaagccggt ttccacataa ttattaataa gatcattcag tgtttttttg
    32161 ctataacgta atgctacagt atcaatgtca tgttttgcaa gattatgcag tatttccacc
    32221 tccactccgg atatgaattt gatattctgc cgttgtgaat cgtacaaaat attccttgct
    32281 acgccattaa ttatcttaac aaaagggaac aaaataaaat aatttttcat cactttgatt
    32341 ttttgaacaa aaaaggtgtt atgggacaaa cgtgctgtct tctaataaca acccttttac
    32401 actcaattgc ccagccgttt tccatattca aaactttaga aaaacctggg tttgctttca
    32461 tgcctttttt ttcaaccata tacgaaaaaa gaaagttttt catgacatta caaattcttg
    32521 tgagttactt aattgctgaa ttttaattag atattcagct attttttttt caatattata
    32581 attacaaggg acacttacca tgtcaaatat gccaacgggg ttaacttcca gaaaaattat
    32641 ttcttcatcc atgctaacaa taaaatcaag agatcctgtt tccaaattca tatccttcat
    32701 aaaactcaaa atttttttct caatatttat aggcattttg tatggagtat atggcaccca
    32761 ctcttcataa gttctgaaat cattcttcgt gctaggagaa aactgagaat gaatcgccat
    32821 tgaaaaaatc atatccccaa atacaaacgc ccttatctcg aaattctttt ctacacaatt
    32881 ttgtatcagt gagtaattaa attgttctgg aatattcgct aatgtatcga catttatcgg
    32941 ctgtgtataa agtatgtgaa attggtcatt atgttcatat ggtaaaaagt cctttacagg
    33001 ttttgttata acattataac actccatttt ttctttgagt tgcttcttgt tataacaaat
    33061 agaagttgtt ggtatcttaa tcccattttt actagccaca atcaaagatt gaagtttatt
    33121 tattgggtat tttaacctat ttccaattga aggctttaat gagaacaaat aatgaataaa
    33181 cgcattgtaa gattgaaatt catcaatttc cattagcttt atacttcgaa tattttcatc
    33241 cttattgtca tcaattacct tctttaagaa aaaatccgtc cttcgccacc aaacagcaga
    33301 aataagatca taatcaacta tttcgcctcg tactttatat acaaatttaa caccaccatc
    33361 ggatacattt aatgacaaaa tatctaaagt gtcttcatcg aaaatcaaag tatatttcgt
    33421 gtttaaaaat tcaagccatt cgcacacgcg aaacacactt ttatcatttc gttctgttaa
    33481 tataagtatc ataataagta ttttataact gcatttagaa atcaggtctc taaatgcagt
    33541 tattgattaa acaaacgata ttttatttag ggctatcata aactctacta acatttgttt
    33601 catctgtgat gatagttgga tctgagctgc cataagttgg atcatagtat tttgtgtctg
    33661 tgccagaact ttggccactt gtccatgttg tcctttcaga gcatataccg cccaatatgt
    33721 gttcttgtcc atttaaaata caagcaagtt cgaaattctc aatgtttttt ttcatgtttc
    33781 tcaaatataa atcccgccta ctctctcaca gcttttccgc ccgaggcgga caggtcggaa
    33841 ccgctgtttc cgccaaacgg cggagttaat ttgt
//
LOCUS       DAOPKE010000023        33425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1395_length_33425_cov_12.325742, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000023 DAOPKE010000000
VERSION     DAOPKE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1395_length_33425_cov_12.325742"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1100)
                     /locus_tag="PKY63_04720"
     CDS             complement(219..1100)
                     /locus_tag="PKY63_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99952.1"
                     /translation="MKRGFVFYCMMIFAATSVVAQYEPDTVFTPGWNLGINTGYYKAS
                     SNHAGYYDGRPQNENNINYVLSNPYWRQDIENELVDETGRDSFWLEEYPVQMKYNAAM
                     MFGFVARYNYSADFSLNFQFNFVKLHLNDVFNLEVWPAIPGNVQSFVPCAITGIESRS
                     NIDFSMMFSFASAKKSPVKPFFEIGVNLNSVNVKSAQIHIFDLTYNIRDVYGGVPYVP
                     NMPLSEYPIKQGGVGYGLYSSVGARYVISKDFWLELLGMINYSTINLEGYQGFGLHWA
                     PVLRLVLSPSMLNAEVY"
     gene            complement(1272..3740)
                     /locus_tag="PKY63_04725"
     CDS             complement(1272..3740)
                     /locus_tag="PKY63_04725"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPE99953.1"
                     /translation="MSLFLFAAAASGQEADTSVIGNDSIKATVQSEKSALTDPIDYSA
                     RDSIHFSVKQQIVEMFGQALVDYGDIHLEAGYIRIDFSKFVIMAYGLPDSNGVVQGIP
                     KFKDGEQEFESDTIVYNYETKKGIIQHIFSEQGGGFLHGDRVKKLPDNTILVRDGSFT
                     TCDQKDPHFQICFHKAKVIPNDKIVTGPVWLEIEDIPTPLALPFGFFPNKKGRASGIL
                     IPSYGESANRGFFLENGGYYFGISDYFDLAVRGDIYSRGSWALKTQSNYKKKYKFDGN
                     LSLSFARNIFGERNTPDFSRNSDFFIKWYHTQDPKARPNSRFSANVQAGTSNYNTYNP
                     SSAQDYLSSNYSSSISYSTTIANLFNFTANLNQSQNRQTHAFDVTLPELSLSSNRIYP
                     FRQKGKTRKSFKALDNITVSYAMNARNSLHTADTSLAEDFNWDNFNNGIKHTIPVSMP
                     LKLFKVVTMNNSFTFNERWYFSTIEKHWSAPDTAEIIDTVRGFSAAHDFAVSSAFTTK
                     MYTFLSFKKGPVSAFRHVLTPSVAFSFRPDFAEQNWGYYKYYFRPGDLAPTQYSIYER
                     GIFGGPAAGKQSMASFSLGNNIEMKVRSKKDTVSGFKKIALIDNLTLNASYNAAADSL
                     RWSMINLSGRTRVFKGLDVRYAAVFNPYVRDTSGRTINTFEWDANHRLFKQENGVWGA
                     SLNYQLNNETFHKKGYKPDSMVLKHFVPTWSLQLSYTLQYSQDFTDNADEPGKVIQTL
                     SFSGDIRLTPNWKVSMMSGYDFVNHDFSYTSVNIHRDLHCWEIIFNWIPAGPRKSYNM
                     TIRVKSSILQDLKLEKKTDWRDQY"
     gene            3849..4955
                     /locus_tag="PKY63_04730"
     CDS             3849..4955
                     /locus_tag="PKY63_04730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPE99954.1"
                     /translation="MKYILFILCLCILMVPSGLMAQNSTSAKWTVVLDAGHGGKDPGA
                     LGKRSKEKDIVLDVVLRVGTYLKNHDNIKVVYTRSTDVFIELDRRARIANEAKADLFV
                     SLHCNANEKTSPNGTEVYVMGLNKTEANLAVAMKENSAALLEDNHEDRYDGIDPNSTE
                     AYIAFSLFQNIYIERSLTMAQLLMKNLNNQLKLYDRGTKQAPFFVLYRTAMPSVLIEA
                     GFISNYNDETILMSDAGKEKIAWSIYNAIIAYSNETENLNNNPVPMRSVEETAVQQNT
                     EMTSDGVIFKIQFASFKEIIPVSDSRFKNLKNVSYKKYGTFYKYFCGETKSYTEALNY
                     LSTVRSNGYKDAFLVAFNNDESISVQDARKLSGQ"
     gene            5007..5993
                     /locus_tag="PKY63_04735"
     CDS             5007..5993
                     /locus_tag="PKY63_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPE99955.1"
                     /translation="MFKKKEVRIALFFILGMVLVYWGINFLKGKDIFSNQTILYAEYE
                     NVTGLQEANPIMLNGYKIGQISEISFKPGGKGKLLVKMLLTEDVDIPSNSIAKIISSD
                     LLGSKAMQIILGNSSEPVESGDYLKGETETDLKEEVSMQILPLKNKAEELLSSFDSVL
                     VILQMIFNDDTRENLSRSFESIKNTIASLEHASYNIDTLVTSQRYRLGNIFANVESIT
                     GNLKENNDKIRQIFTNLETISDSLTKSNFKATINNANESLAKFNEVMTKVNQGQGSLG
                     LLINNDTLYYNLERSSEDLDKLVEDMRLNPQRYLHFSIFGRSGKRNAYTPRD"
     gene            complement(6097..6804)
                     /locus_tag="PKY63_04740"
     CDS             complement(6097..6804)
                     /locus_tag="PKY63_04740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPE99956.1"
                     /translation="MEENVNSMPRIGEKAPEFKAVTTQGDINFPADYKGSWVILFSHP
                     ADFTPVCTSEFMTFATMEKQFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKNV
                     EVTFPLIEDITMEVAQKYGMMMPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLGRNFD
                     ELLRVVIALQAADEFGVATPADWRPGDEVIVPTAGSCGVAKERMENKAGDTHCYDWFF
                     CTKKLDKDTVLKKILKK"
     gene            complement(6831..7343)
                     /locus_tag="PKY63_04745"
     CDS             complement(6831..7343)
                     /locus_tag="PKY63_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPE99957.1"
                     /translation="MGKEAIKVSQVDVNKLIEMLNAALAEEWLAYYQYWIGARVMEGP
                     MRSEVEPELLLHATQELAHAELVAERIIQLGGTPLINPDQWKKHARCSYDEPADPYIE
                     VILDQNLKGERCAIQRYQEIADFTAGKDHSTHQMAVQIMNEELEHENDIEAWITDLNR
                     MKEEWKKMRL"
     gene            complement(7379..7966)
                     /locus_tag="PKY63_04750"
     CDS             complement(7379..7966)
                     /locus_tag="PKY63_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99958.1"
                     /translation="MRIFSTLVVLLLVSGRVFADSPLTSTDFHKAYSGSSVVQSAMKS
                     NGELTPELMNVLNSKKFSIAVKVAVINAIGWNIDGQNNFQTYKNFLGAKTGKGKLKAE
                     NMLCLAYLKAMDNYFDCNDALKMADEALALNPMSYTFNIIHALIRAQVLFDVNWCEVW
                     NATDAVRKNQLLNKDMNAEAIRIIFEYMDLYQAEC"
     gene            complement(8027..10561)
                     /gene="feoB"
                     /locus_tag="PKY63_04755"
     CDS             complement(8027..10561)
                     /gene="feoB"
                     /locus_tag="PKY63_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPE99959.1"
                     /translation="MTLNDLNPGQIGYVVKVRGSGAFRKRVIEMGFTPGRRIEVVRKA
                     PLQDPVEYRLMDYEISLRRSESRSIEVVTEEMLQFEESEMNSAGTTSSNLAYEVLERK
                     AILRSKEIDVAIVGNPNCGKTTLFNFASGSTEHVGNYSGVTVEAKTGSFRFRDYKINL
                     IDLPGTYSLSAYSPEELFVRKQLISSPPDFIINVIDASNLERNLFLTTQLMELDIPVV
                     IALNMHDELLAKGDYFDYKSLGEMLGIPFQPTVGHRNKGVHELLSKVVELYENQSLHV
                     RKAEIRFVMEIENAIERISEQMCESHFLKEMVSSRFYAIKLLENDREALAAASRKDFE
                     KVHDVARKESETISAIYHSDTESLIADARYGFIAGALKETYRSGVRKDSFSSATQRID
                     RILTHRFLGFPIFLFFIWLMFFSTFFVGSFPMHWMELGVGWIGEQIAAVMHDGWFKDL
                     MVDGIIGGVGGVLVFLPNILILFLFIAIMEDTGYMARIAFLMDKVMHRIGLHGKSFIP
                     MIMGFGCNVPAIMATRTLENPTDRLLTILINPFMSCSARLPVYILVIGALFPQNAVWM
                     LFLVYAIGVLFAVLFSLFFKKTIFRKTEAPFVMELPPYRMPTHHAILRHMWFRASQYL
                     KKMGGIILIASVLIWALGYFPRKNEPSPQLSEKLALIEAQMQKNRETEVVWPGEKDSV
                     ALAGEHQLVLQQIESERQENSYIGRIGKFIQPVMEPLGFDWRLSVSLVAGVAAKEVVV
                     STMSVLFSNEHNDKGGRISAIKHPAGKTLGEQLVSDAGGKPVLNPVTGFAFLMFVLLY
                     FPCVAVVAAIRKETGGWKWALFTVVYTTAVAWFVAFGVVKIGGLLF"
     gene            complement(10727..12427)
                     /locus_tag="PKY63_04760"
     CDS             complement(10727..12427)
                     /locus_tag="PKY63_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPE99960.1"
                     /translation="MRILSSFVLMLFAFASMAQKVSQSEALTLAQNFMTSKASRSISL
                     KPVSVETKGMNHLYYFTDESNTSFVVISSEKTVTPVIAYSFESRLDAQLPAPVMDYYI
                     GVNAGIEKAISEKAVPDALVEAEWKSLSKYGELPVYLKSAVTPLLSTTWNQDCYYNDS
                     VPEAATGPCGHCYAGCVATAMGQVMKYHNYPPVGTGSNIYGTGNYSNIHVDFSQASYN
                     WAAMPNSITTNNSYIAQLLYHAGVSVNMGYDFDGSGASSQDAAYAFADHFGYADYAFF
                     AERDNTSDEFWIQLIDNELRCRRPVYYSGHSSSGGHAFVCDGIDNSNRLHINWGWGGS
                     SNGYFMLSSMLGFNDGQAAIFGVEPAIGDVEYCQPSSVYTAMTDTIEDGSGVNKYGNG
                     TNCSWTIQPADAGLIYIHFTEVALDKDMDVVYIYNGTSTSDPMVTSVTGFELPSEILV
                     WGPSAHIVFQSDNLLRADGFKLFYTTCLAGIEEAWNEGKISVFPNPADEYMNIEIDPM
                     ILPAVIRIELVSMSGQTIMVVDNPVQSELLEVGSLSEGTYGLRFIGESDSFSAFIQIQ
                     "
     gene            complement(12655..13419)
                     /locus_tag="PKY63_04765"
     CDS             complement(12655..13419)
                     /locus_tag="PKY63_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPE99961.1"
                     /translation="MKVVTYNLNGIRAAIGKGFLEWLTQSDADVVCVQETKAQPDQIP
                     VMEFEALGYKTYFYSAQKKGYSGVGIITRIQPDKVVAGMGIAEYDYEGRFIRADFGDL
                     SIVSAYHPSGTSGDERQAFKMTWLDRYLDYVQELRKERPNLLLCGDYNIAHEKIDIHD
                     PVGNAKSSGFLPEEREWLTKFLSHEYIDSFRHFNKEPHNYTWWSFRANARANNKGWRI
                     DYIMATESLRSKLKSAEIWPDAMHSDHCPSVLDIDF"
     gene            complement(13422..15188)
                     /locus_tag="PKY63_04770"
     CDS             complement(13422..15188)
                     /locus_tag="PKY63_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPE99962.1"
                     /translation="MRTVLIIPLFFLFASHVEAQTKTFSIPYESKGQNVTMTYDEIIH
                     YCRLTDSLYSEVSFKSFGKSSQGDDLPYLMIDDGSKTTDKITLWIQAGIHPGEPEGVE
                     AGFLFLRDLMTKPEMRPMLRNVRILFIPCFNVDGLKRFGPYNRINQNGPEQMGWRTNA
                     QNLNLNRDFMKADAPEMQAWLKLYNEINPHFLIDCHTTDGADYQYPLTYGLDAFQTLN
                     PTVAAWCTKKYIPFVSEKMNRDGMPIFPYVAFRQWHNPRSGLITYGSRPMLSHGYTIL
                     SDRPCLLIETHMLKPYKVRVNATKNMLFHTFKYLSDNHESYMSILRGADQERVSDKFA
                     LSPFPIALATDMNDSTIIDFLGMQFDTLKSGITGGIYYKYDNTKPVTWKIPLFDKVYV
                     SDSIKLPLAYVIPVEWVDVIERVKLHGISVITSSKDVALHVTVSKLNNVKFPKTCFEG
                     RFQPSFNIQEFDTTMNIPKGSALVLVKQPKIKVLVWLLDAASEDSFLKWGFFNAIFEQ
                     KEYGEMYVLEPMAEKMFDENPALKAEFEALKKSDSVFASNQWLQMNWVYNHTVYRDQL
                     YCVYPVLKITDQTELEKLLPQK"
     gene            complement(15414..17546)
                     /locus_tag="PKY63_04775"
     CDS             complement(15414..17546)
                     /locus_tag="PKY63_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPE99963.1"
                     /translation="MKSFTDIICIELGLKPVQVANTIKLLDEGATVPFIARYRKEMTG
                     TLDEVQILAIKTRLHQLTELEKRKAAVLESISEQGKLTPELEDKIKNAMTMPEVEDLY
                     LPYKPKRKTRATVAIAKGLEPLAKRIFDQQPFDIMEAARHFISEEKGVVSEEEAIAGA
                     RDIIAEWISEDARVRADLRALFIREGVIYSKVAKGKETEGAKFENYFSISEPLAKAPS
                     HRILAIFRGENEGFLRVSVEPDEERALGLIDRHIVKAKNAIAEEVQKAVDDSYNRLLQ
                     PSLETEVRKFYKEKADKEAIAVFADNVRQLLLASPLGQKMVLAIDPGFRTGCKVVVLD
                     PQGNLLHNETIYPHPPESKVKEALNKIDHLVEAYKIEAIAIGNGTAGRETEAFIKRIH
                     FSRPVIALAVNESGASIYSASDVAREEFPDYDITVRGAVSIGRRLMDPLAELVKIDAK
                     SIGVGQYQHDVDQNMLKEKLDETVMSAVNNVGVELNTASKQLLTYVSGIGPAMAQNIV
                     EHRKQKGAFKTRKDLLKVKRFGDKAFEQAAGFLRIKDAANPLDASAVHPESYGIVEKM
                     AKDMNCTVNDLIQNKELRAQIKPQNYITEKFGLPTINDILKELEKPGRDPREKFEIFE
                     FDQNVHSIDDLIAGMELPGIVTNITKFGAFVDIGVHQDGLVHISQIADRFIADPSEVL
                     KLNQKVKVRILEVDKPRKRINLSMKGVQ"
     gene            17732..19159
                     /locus_tag="PKY63_04780"
     CDS             17732..19159
                     /locus_tag="PKY63_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99964.1"
                     /translation="MCDIVGRDEEIEIMRDLEQSRVSEFLVVYGRRRIGKTYLIREVF
                     QNKFVFSITGIDNVNMTQQLMNFNLTLRQHDADGEWEPVNCWLLAFNQLRKFLEKSKK
                     KRKIVFFDELPWFDTQHSDFIQGLEHFWNSWASGRKDVFLIVSGSATSWMINKLINNR
                     GGLHNRVTQRICLEPFTLAECSEFMKRKNVHLDQYQLIQLYMVMGGVPYYWNKVTRGK
                     SAAQIIDRECFSRNGSLKDEFNNIFKSLFRHHQRHISIVKALATKSKGLNRNKISKLS
                     GISNGGGLTRLLDELEACHFITRYIPFGRTSRDSLYQLNDMFSLFHLRFMHTSTKKDA
                     GNWLKTIDSPKHRAWSGYAFEQVCMQHSAQIKKALGISGIETHISSWRSRSDDSGAQA
                     DMIIDRRDQIINLCEMKYSVNPYIINKKYATELSEKAGRFRYDTKTKKAVHIVLLTTF
                     GVANMEHYGGIIQHNLTMDCLFEKA"
     gene            19237..20850
                     /locus_tag="PKY63_04785"
     CDS             19237..20850
                     /locus_tag="PKY63_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPE99965.1"
                     /translation="MSDQLKKKRQIPSTYVIVFTIILIAAAATWLVPGGSFEREKVMV
                     NGVERDIVVQGSYHETETNPQSWEILASVYDGFIDKADIIVFILVIGASFWIINSSKA
                     LDIGIIKFLSRAERLEKYKIFRKIGIRNILITLIMLMFSVFGAVFGMSEETIAFVIIF
                     VPMAIKMGYDSIVGVSMCFVGAAMGFAGALFNPFTIGIAQGIAQIPLFSGLEYRLFMW
                     IVLNIFGISYILIYANKVYKNPQRSVVYETDQYWRDQATNNEHPVETKAGISAWVSFG
                     IILFSLIAFWTKYPYTDITVGSFTVTIPALPILTFLFAIPGIWLLVRKSVQLYILHIM
                     LFTTISLVIGVLGFHWYIREIAALFFGMGLMGGIAANYRPNEFTKHFMDGAKDIFSAA
                     LIVGLAGGIIIILQEGGIVDTLLNSLSSLFSHNKYSAVSGMYVVQTAINLIMPSGSAK
                     AALTMPLMAPFSDLIGISRQASVIAFQLGDGITNMITPVSGVLLGVLSMAKIPYERWF
                     KWAWKFILMLIILGFLLLIPTVTMTLNGF"
     assembly_gap    20944..21043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21096..22334)
                     /locus_tag="PKY63_04790"
     CDS             complement(21096..22334)
                     /locus_tag="PKY63_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE99966.1"
                     /translation="MKHFLFVAAFIYSFSLFAQQSANDYYDAGTAKLNAKDYAGAIPL
                     FTKCIEISPYYYEAYVDRGRCQFALGKKDLALIDYNLALTKKKDFYFAFYYRALWYKD
                     AKQMKEALADITQAVNIRPKSADPYILRAAWYAEQNNNDAALADYNKILEIRPDDPAT
                     LVSRGKIYFNKDMQEQAFNDFSKVIDKNKTYGEAFYWRGVLLEKVERFQVAIEDYTAA
                     INNKFVNEDVYRRRYKLCMDAKQYETAISDLTVLLTTYAPKDASLWNARGLCYAATNQ
                     HLLAIADYNKAVMYNRDFEDAYINRADSKVATNKEASALTDYNKIITINPKNGYAWEG
                     RGVYYFKKKNFTAATEDLDKAIKYSPESGRAYFYRGAIRDMNGDKKGACEDLQKAVDL
                     KYDGSVEAKKRACGDDEGAF"
     gene            complement(22549..23601)
                     /locus_tag="PKY63_04795"
     CDS             complement(22549..23601)
                     /locus_tag="PKY63_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPE99967.1"
                     /translation="MKHFATLILFALFSVTCIAQNDAVTVYDNENFTGDSRNLYSHYN
                     PEGLSDVFINRIGSIRIPTGTWIVIFDESGFRGHYLVIENDWTATGDFAPWNNNISSI
                     LVMRDAQSGLPIPQSNNYNTSDSYNNSNNYNNNNYNYDNSENYNNDVQNNYIEPQVQN
                     VYIPQNSGVYYNIEAAARNPEDVIVLDVRGQNLSTWPSFFSDMENLRELYISNNNFSS
                     WPSFFSDLRQLRVIEAENNNFTSFPSYFSELKNLEILDLSGNPITSFASFFYELKSLR
                     VLDMSYTKVSSFPSFFNELKNLESLSIAHTNIISLPSFFDELTQLRWMDISGTGITDL
                     PYGVRSMNILIVGRPE"
     gene            24018..24713
                     /locus_tag="PKY63_04800"
     CDS             24018..24713
                     /locus_tag="PKY63_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPE99968.1"
                     /translation="MRKIKVCLYFTFLLLPAISFCQTNYLEAGISGGSGIRILSGEYI
                     DNKYTDPAIGYHFGGTIQYHFGKIFSLRTDPSFEQKGNTDYLILTDNVGNEIGHLDAK
                     NNLNYIVLPVLGQLSLGNRIVFTIHAGAFAGYLIRNNHIMPAFETYAEQKINNTASHK
                     RFDIGLAGGFGCQFPLKENFSIFMDARVNAGIFDIQKSPLTGPVYTNSLNVTLGIKYK
                     FTKRTDLKESAIN"
     gene            complement(24835..27444)
                     /gene="alaS"
                     /locus_tag="PKY63_04805"
     CDS             complement(24835..27444)
                     /gene="alaS"
                     /locus_tag="PKY63_04805"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPE99969.1"
                     /translation="MTSNEIRKAFLDFFASKSHSIVPSAPMVLKNDPTLMFTNAGMNQ
                     FKDYFLGNEIPKSKRVADSQKCLRVSGKHNDLEEVGIDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWELLTDVFKLDKDRLFVTYFGGDEHEKLDPDTEAADFWKTHIAKDRILPGS
                     KKDNFWEMGDTGPCGPCSEIHVDLRTDAERASVDGKTLVNNDHPQVIEIWNLVFIQYN
                     RNASGKLEPLPAKHVDTGMGFERLVRAIQNKTSNYDTDVFTPLIAEISSLSGIPYGKD
                     EKNDIAMRVIADHMRAVSFAIADGQLPSNAKAGYVIRRILRRAVRYGYSFLGFTEPFM
                     HKLLGVLEKQMGTQFTELVKQKELIGNVIREEENSFLRTLATGVHMFENYVSKLAGSK
                     TIDGKFAFELYDTYGFPIDLTLLMARERNLDVQIDEFNTYLSEQKNRSRKDAAVAAGD
                     WVEIIPDVVTEFTGYDHLTDEVRIARYRSVEQKGKTIFQIVLDKTPFYAESGGQVGDS
                     GLLQSEKDEIEIINTIKENRLIIHVTDTLPEDPKAIFIAAVNAENRMDTMRNHSATHL
                     LHEALRAVLGTHVEQKGSLVSPEYLRFDFSHFQKLSNEELLKVERMVNEKIRENISLD
                     EYREMKQEEALAMGAMALFGEKYGDKVRVIRFGSSVELCGGTHIPSTGMIGYFKIVSE
                     SAIAAGVRRIEAVSGSVAESMIEKMDEQLRGLKEIFKGKDPVVAVTELNEKLSGLQKE
                     IETLKKEKLVSLAPALLAEAKDMGGIKFLGKILDAPTGDLKDIAFGLRTLSDNLVAVL
                     CSANGEKAGITLLISDKLVKDKGWNAGQLIREIAKPINGGGGGQPFLASAGGTNKEGL
                     NEAIAVAEAVFSK"
     gene            27522..28481
                     /locus_tag="PKY63_04810"
     CDS             27522..28481
                     /locus_tag="PKY63_04810"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPE99970.1"
                     /translation="MSDQHLRFNPQTMHYEVVYKKHSRWRNVLYILLVGSAFGLVFIF
                     LGTTFIKSKEVRKKEHDLAVFKETYEKAELQYQQNIELLHNLEMQDRRLFREIFETDP
                     ADTTLLSNQLLEEAQIDSENTDISGMFSMIKAQYKKIENISYKDAIRLQFASYFLKNN
                     SDYIKSLPVRVPLKSGSYTLVSGFGKRIHPIFKTARQHNGLDLAARAGSEVLASGDGI
                     VQDVPKNLAGYGNVVYIYHGHGYHSLYAQLLESRVKPGQKIHAGDVIGFVGSTGISIG
                     PHLHYEIWKNGKPVDPIRFLLTIAPGEYLEMYKVATQYNQCLS"
     gene            28481..29827
                     /locus_tag="PKY63_04815"
     CDS             28481..29827
                     /locus_tag="PKY63_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPE99971.1"
                     /translation="MNKELKKLTLFTAINFVIANMVGTGVFTSLGFQLFDIKNPAAIL
                     ILWAIGGIMALFGSFVYGELGAAMPRSGGEYNFLSRIYHPSLGFLAGWISATIGFAAP
                     VAAACIAFGTYYHQSIDILSNTYSVNWLQTTPMIAGLIILALITMVHSNSIKTGGRFQ
                     NVFTILKLLFIVIFIGAGLFFLPESQTLSFAFSADTGNDILNPAFAVALVFVSYAYSG
                     WNASAYFVNDLKNPVKQLPRSLIIGSIAVMILYILLNFVFLISTPIEEMIGNPEIGLI
                     SAMYIFGNKMGVFMGLFISLLLVSSISSMIFAGPRVTMVMGEDFSLLKFLARKNKNEI
                     PYIALITQFIISFTLIITSTFESLITYTGFVLNLFTFFTVLGVFVHRKKFPDADRPVK
                     TWGYPVTPIIFLIFNLWITIFVIYSRPLESLLGLATIAVGAILYFFSKQSNKPKTI"
     gene            29824..30990
                     /locus_tag="PKY63_04820"
     CDS             29824..30990
                     /locus_tag="PKY63_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99972.1"
                     /translation="MKTSLFILMLSIFFMISCSGSSKDKQNGADSTTTDTVTKTEAIV
                     LNHYYNDIARFVAGMPLSDSSLLKKYTEKTEWKNYSAGIDSSWANFQKTKLDVMQPWI
                     NKELNAINEKTKDVFYPFSGPDFVYMYHFLPKADNYYMVALEPVGIIPDITKIENSMP
                     SFFAALNNAIKDNLNLSFFITKSMKVQMNNEQIKGTIPVLLFFMARMNLHIQNIIPAT
                     IAKDGKLSVNEQIAENSANKKFTNGVEISFVRPGENKVNKLYYFSVSIRNDGFASMPE
                     AETYLKSLPADMTTIVKSSSYCMHEDKYNKIRDIVLTHSKFLIQDDSGVPFRFFNKEK
                     WNFGYYGIYTRPIPVFSHFYQDELKKTWPQNATKLSFRFGYNEESNIFVATRKN"
     gene            30994..32019
                     /locus_tag="PKY63_04825"
     CDS             30994..32019
                     /locus_tag="PKY63_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPE99973.1"
                     /translation="MSKTKITTILIAFSGIILLSLPSSGQFDSTKIIDTIESNGKQIV
                     LFSDNVWSYISSMEDLAHDKAIADTGRVFTEYWNDLTFAYMYPEDKPLPDTVQIVLID
                     SLHSFTPPLLGGITSGFGWRSGRVHKAVDIGLDFGTPIRSAFDGKVRYAQYNTGGYGN
                     LIIIRHFNGLETYYAHLSKMYVRPGQMVKAGQVIAGGGNTGAHWSGPHLHFECRYRDH
                     AFDPLKIMRFDSTELKSDTLILTAADFKVSQNHKAYLADGVNGTNTGTSYSNTRQYGG
                     STSDNSDYKGSSANGTAIGNYYIVKKGDTLSAIARKYGTTVDKLCKINGINKNAILRL
                     GQKIRVR"
     gene            32019..33305
                     /locus_tag="PKY63_04830"
     CDS             32019..33305
                     /locus_tag="PKY63_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPE99974.1"
                     /translation="MFGRFFIFVFCLLGVTVAYSQDWPKERDTSCLPGKIIFEQYCDS
                     FPEIYMMPEWYKLQLIYVQVNRDKNGIPHLTYHKLGVDRNKYFYPASLVKLPTLLLTL
                     EKLQSLKSRGVDKYTRLSIGKEHSCQYEFKTDTSGIEDYPCIANFIRRILLVSDNWSY
                     NRLFEFVGQKELNEGLRRKGYTDPVILKRFARCDDDENRYTNPFCFYNDDGNILLQQP
                     QLENRTEYRLPPHVNASVGKGFWDGKKVLEQPRDFSNNNYLPLEDALEMLISFIMPEA
                     VDEQKRWNLSPHDRLFLLQYFSMYPRESLFEEYHNWPKYEDSYKKYFLMGDHKDTIYD
                     GALRVFNVVGLSYGFASDVAYVVNLREGVEFFLAATIYTNHDDILNDETYEYRNIAFP
                     FFGRFGRIIYDMERAREKQYLPDFREIKQALSLPPM"
BASE COUNT         9750 a         7480 c         6724 g         9371 t
ORIGIN      
        1 gtttgatttc cataaagtta cataaacaaa acgacatatc tatctgatag tcaatggttt
       61 attaacattt ttatcaacaa aaatggcaat aataattttg attttgaaat tttagacagt
      121 tacttatgat agaaagaaaa agcgtaaaaa aacaaaaccg tatcattaca tttttttcac
      181 aaaattaaat ttttcttttt attattttaa aaattacatc agtaaacttc agcattcagc
      241 atggaaggac tcagcaccaa tctcagaacc ggcgcccagt ggagcccaaa gccctgatac
      301 ccttcgaggt taatggtaga ataattgatc atgcccagca gctcaagcca gaaatctttc
      361 gatatgacat agcgggcacc caccgaagaa tacagcccat aacccactcc gccctgctta
      421 atcggatatt ccgacaacgg catattaggc acatagggta ctccaccata gacatcacgg
      481 atattgtagg taagatcgaa aatgtgtatc tgagcacttt ttacgttcac actgttcagg
      541 ttcacaccaa tctcaaaaaa cggcttcacg ggcgactttt tcgctgaagc aaaagaaaac
      601 atcattgaaa aatcgatatt cgatcgcgat tcaattcccg taatggcaca gggcacaaac
      661 gattggacat tgcccgggat ggctggccac acctcaagat tgaaaacatc attcaaatga
      721 agttttacaa aattgaattg aaaattcaaa gaaaaatcag cagaataatt gtagcgtgca
      781 acaaaaccaa acatcatggc cgcattgtat ttcatctgca ccggatactc ctcgagccag
      841 aacgaatcgc gtccggtttc atctaccagc tcattttcga tatcctgccg ccaatacgga
      901 ttgctcaaaa cataattaat gttgttttcg ttttgcggcc gtccatcgta atagcccgca
      961 tgatttgacg acgccttgta atagcccgta ttaatgccca gattccagcc cggcgtaaac
     1021 accgtatccg gctcgtactg cgccacaaca gatgttgcag caaaaatcat catgcaataa
     1081 aaaacaaaac ctcttttcat tccgataatt tttttgtcct ttttgtgttt ccgtggctga
     1141 gccccgggct gatatctgca gcgctttcag cgctatttgt gcttcgcagc ttttttatgc
     1201 tctcagctcc tcaaccttaa tctcatcctt aacctgtcac cctgagcgga gtcgaaggac
     1261 actactcaca actaatactg gtctctccaa tccgtcttct tctcaagttt cagatcctgc
     1321 aatatacttg atttgacccg gattgtcatg ttatagcttt tacgcggccc agcaggaatc
     1381 cagttgaaaa tgatttccca gcaatgcaga tcacgatgaa tgttcaccga ggtataactg
     1441 aaatcatgat tcacgaaatc gtatcccgac atcatgctca ctttccagtt cggtgtcagt
     1501 ctgatatcgc cactgaaaga aagtgtctga atcacttttc cgggctcatc cgcgttatcg
     1561 gtgaaatcct gtgaatactg caaagtatag gatagctgca gcgaccacgt gggaacaaaa
     1621 tgcttcaaga ccatcgaatc cggcttgtat cctttcttat gaaaagtctc attgttgagc
     1681 tgataattca gactggcgcc ccagactccg ttttcctgtt taaacagacg atgattcgca
     1741 tcccactcaa acgtattgat ggtgcgcccc gacgtgtccc ggacatatgg attgaatacc
     1801 gccgcataac gcacatcgag ccccttgaac actcttgtac gacctgacaa attgatcatg
     1861 gaccagcgca gactgtcggc tgcagcattg taacttgcat tcagtgtcag attatcgata
     1921 agcgcaattt ttttgaatcc gctaaccgta tctttctttg agcgtacttt catttcaata
     1981 ttattgccca gcgaaaaact ggccatggat tgttttcctg cagccggccc accaaaaatg
     2041 cctcgttcgt aaatggagta ttgtgtcgga gccagatcgc ccggcctgaa ataatacttg
     2101 taatagcccc agttctgttc ggcaaaatcg ggccggaacg aaaaagcaac ggaaggtgtt
     2161 agtacatgac gaaacgcaga caccgggccc tttttaaatg atagaaacgt gtacattttt
     2221 gttgtaaagg ccgacgaaac agcaaaatca tgagcggcac tgaacccccg caccgtgtct
     2281 atgatttcag ctgtatcagg ggcactccag tgtttttcga tggtgctaaa ataccagcgt
     2341 tcgttgaacg tgaatgaatt attcatggtc accactttaa acaatttcaa cggcatcgaa
     2401 accggtatgg tgtgcttgat tccattgttg aaattatccc agttaaaatc ttccgccagc
     2461 gatgtatcag ccgtatgcaa actattgcgg gcattcatag cataagagac tgtgatattg
     2521 tcaagcgctt tgaaagattt tcgtgtcttt cctttttgcc tgaaaggata aatccggttc
     2581 gatgaaagcg aaagttcagg cagggttaca tcaaaagcat gtgtttgtcg gttctggctc
     2641 tgattaaggt ttgctgtaaa attgaacaga tttgcaatgg ttgtgctgta ggaaatactg
     2701 gatgaataat tcgatgaaag ataatcctgc gcagatgaag gattatacgt gttgtaattc
     2761 gaagttccgg cctgtacatt ggccgaaaag cggctattgg ggcgggcttt cggatcctgt
     2821 gtatgatacc atttgataaa gaaatcactg tttctgctaa aatcaggcgt atttcgttcg
     2881 ccgaaaatat tacgtgcaaa actcagactg agattgccat caaatttgta ttttttcttg
     2941 taattcgatt gagttttcag cgcccagctt ccacgcgaat aaatgtcgcc tcggacagcc
     3001 agatcgaaat aatcgctgat accaaaataa taaccaccgt tttcaagaaa aaaaccacga
     3061 ttagcactct caccataaga aggaattaat atacccgacg cacgcccctt cttgtttggg
     3121 aaaaacccga acggcagcgc caatggagtg ggaatatctt caatttcaag ccagaccgga
     3181 cctgtaacaa ttttatcgtt cggaattacc ttggctttat gaaaacatat ctgaaagtgt
     3241 gggtctttct gatcgcaggt agtgaacgat ccatcgcgca ccagaatagt attgtcagga
     3301 agttttttta cacggtcgcc atgcagaaaa ccgccaccct gctcggaaaa aatatgctga
     3361 atgatccctt tctttgtttc gtaattatac acaatggtgt ccgattcaaa ttcctgctct
     3421 ccatccttga atttgggaat accctgtacc acaccgtttg aatcaggcag accataggcc
     3481 atgatgacga attttgagaa atcaatcctg atataaccag cttcaagatg aatatcgccg
     3541 taatcaacca aggcctgtcc aaacatctca acaatctgct gcttcacact aaaatgtata
     3601 gaatcccgcg ctgaataatc aatgggatcg gtcaatgcac ttttctctga ctgaactgtc
     3661 gctttgatgc tatcgttccc aataactgaa gtatcggctt cctgccccga tgccgcagcg
     3721 gcaaacagaa acaaggacat aacaatattt aaaaaccgca aactcaattt atggtacaat
     3781 tgctgataag gaaaaaaccc cttacatttg taacgggatt cggattacaa aaataattat
     3841 aatcgcggat gaagtacatt ctttttattt tatgtctgtg catcttgatg gtgccaagtg
     3901 gcctgatggc tcagaattca acatcggcaa agtggacagt ggttctcgat gctggccatg
     3961 gaggcaaaga tccgggcgcg cttggaaaac gcagcaagga aaaagatatt gtgctcgatg
     4021 tggttttgcg tgttggtact tatcttaaaa accacgacaa catcaaagtg gtttataccc
     4081 gcagcaccga tgtttttatc gaactagacc gccgcgcccg gattgccaac gaagcaaaag
     4141 ccgacctgtt tgtctcattg cattgcaacg ccaatgaaaa aacttcgccc aacggcaccg
     4201 aagtatatgt aatggggctc aataaaaccg aggccaatct ggctgttgcc atgaaagaaa
     4261 attcagctgc cttactcgaa gacaatcacg aagaccgcta cgatggcatc gacccgaatt
     4321 caaccgaagc atatattgct ttcagtcttt ttcagaacat ttacattgaa cgcagtctga
     4381 caatggctca gttgctaatg aaaaacctga acaaccagtt aaaactatat gaccgcggaa
     4441 ccaaacaggc acctttcttt gtgttgtatc gaacggccat gccttctgtt ttgatagaag
     4501 ccggcttcat ttccaactac aacgatgaaa caatcctgat gtctgatgcc ggcaaggaaa
     4561 aaatagcatg gtccatctac aatgccatca ttgcctatag caatgaaact gaaaatctga
     4621 acaataaccc tgttccgatg cgctcagtag aagaaacggc tgttcagcaa aacacagaaa
     4681 tgacatcaga tggcgtaatt tttaaaatac aatttgcatc attcaaggaa ataatccctg
     4741 tttcggactc acgttttaaa aatctgaaaa atgtttcgta taaaaaatac ggcacattct
     4801 acaaatactt ctgtggcgaa acaaaatcat ataccgaagc actgaactat ctttcaacag
     4861 tgcgaagcaa cggctataag gatgcttttc tggttgcttt caacaatgat gaatccatca
     4921 gtgtgcaaga cgccagaaaa ctgagcggac agtaattttt attgtccgct gaaaaaaatc
     4981 cattagattt gtaaaaaaaa ccaaaagtgt ttaagaaaaa ggaagtacgg attgcattgt
     5041 ttttcattct cggaatggtg cttgtttatt ggggtatcaa ctttctgaaa ggaaaggata
     5101 tcttttccaa tcaaaccatt ttgtatgccg aatacgagaa cgtaaccggt ttacaggaag
     5161 ccaatccgat tatgctgaat ggatacaaaa tcggacaaat ttcggaaatc agttttaagc
     5221 caggtggcaa aggcaagctg ctggtaaaaa tgctgctcac agaggatgta gatattccat
     5281 cgaattccat tgcaaaaatt atcagcagcg acctgctcgg aagcaaagcc atgcagatta
     5341 ttctcggtaa ttcatcagag cccgttgaaa gcggagatta tctgaaaggc gaaacagaaa
     5401 ccgatctgaa agaagaagta agcatgcaga ttctgcccct gaaaaacaaa gccgaagaat
     5461 tgctatcctc tttcgacagc gtacttgtaa ttctgcaaat gatcttcaat gatgataccc
     5521 gcgaaaatct gagccgctcg ttcgaaagca tcaaaaacac cattgccagt ctcgagcacg
     5581 catcttacaa catcgatact ctggttacat cacagcgtta caggttgggc aatatatttg
     5641 caaatgtcga atccatcacg ggcaatctga aagaaaacaa cgataaaatc agacagattt
     5701 ttaccaatct tgaaaccatt tctgacagtc tgacaaaatc aaatttcaaa gccaccatca
     5761 acaatgccaa tgaatcgctg gccaaattca atgaagtgat gaccaaggtc aatcagggac
     5821 aaggttcgct cggtttgctc ataaacaacg acacattgta ttacaatctt gaaagatctt
     5881 ctgaagatct cgataaactg gtggaagata tgcgtctgaa tccgcagcga tatctgcatt
     5941 tcagcatttt tggacgcagt ggaaaacgca acgcctacac tccacgcgat taaacaataa
     6001 aaaatattca tgataaaaaa atagcacgga ttattaatct gcgctatttt ttttacatac
     6061 ttggcgtgga tttacaatcc atgccctata tgtaaattac tttttcagaa ttttcttcag
     6121 tacagtatcc ttatccagtt tctttgtaca gaagaaccaa tcgtagcaat gggtatcgcc
     6181 tgctttattt tccatacgtt ccttggcaac accgcatgag ccagctgtag gcacaataac
     6241 ctcgtcgccc ggacgccagt cggcaggagt agcaacacca aattcatcgg ctgcctgcag
     6301 agcaataaca acgcgcagaa gctcgtcgaa attacggcca agacttagcg gataataaat
     6361 aatagtgcga atgattcctt tagggtcaat gacaaatacg gcgcgaaccg ctttggtatt
     6421 gctttcgcca ggcatcatca taccatattt ctgtgcaact tccatggtaa tatcttcgat
     6481 gagcggaaaa gtaacctcga catttttcat gcctttgtat tcaatctttt ccttgatggt
     6541 gcgcaaccat gcaatgtggc tgtacaagcc atctaccgaa agtccgacga gcttacagtt
     6601 ggcctcgttg aactgttttt ccattgtagc aaaagtcatg aactccgaag tacaaactgg
     6661 tgtaaaatcg gctggatgac tgaaaagtat aacccagctt cccttgtagt cggctggaaa
     6721 attaatatca ccctgagttg ttacggcctt gaattcaggt gctttttcac caatacgagg
     6781 cattgaattt acgttttctt ccataattgt ttttttgttt tggttaataa ttaaagtctc
     6841 attttcttcc attcttcttt catccgattc aggtctgtga tccatgcctc gatatcattt
     6901 tcatgctcaa gttcttcatt cataatctga accgccatct gatgcgtaga atggtcttta
     6961 ccggcagtaa agtcggcgat ttcctgataa cgctgaatgg cgcaacgctc gcccttcaga
     7021 ttctggtcaa gaataacttc gatgtacgga tcggccggct catcgtagga gcatcgggca
     7081 tgttttttcc attgatcagg attgatcaga ggcgttcctc ccagctgaat gatacgttcg
     7141 gcaaccaatt cggcatgtgc cagttcctgc gttgcatgca acagcaattc cggttcaact
     7201 tcgctgcgca tcgggccttc catcacacgt gcaccaatcc agtactgata ataggcaagc
     7261 cactcttcgg ccaatgccgc attcaacatt tcaatcaatt tgttcacatc aacctgtgat
     7321 actttaatag cttcttttcc catagttttg atttttttag tttttatatt ttgttttact
     7381 aacactctgc ctgatacaga tccatatact caaaaatgat gcgaatggcc tctgcattca
     7441 tgtctttgtt caacagctga tttttacgca ctgcatcggt agcattccat acttcgcacc
     7501 agttcacatc aaacaatacc tgcgccctga taagagcgtg aatgatgttg aaagtataag
     7561 acattggatt gagtgccaac gcttcatcag ccattttcaa agcatcattg caatcaaagt
     7621 aattatccat ggctttgaga taggccagac acagcatgtt ttcggcttta agttttccct
     7681 tgcctgtttt agcgccaaga aaatttttat atgtctgaaa attattctgt ccatctatat
     7741 tccatccaat agcattgatt actgccactt taacggcaat gctgaatttt ttcgagttca
     7801 atacattcat aagttccgga gtcagttctc cgttgctttt catagctgac tgcacaacag
     7861 aagagccgga ataagcctta tgaaaatcag tggatgtcaa aggcgaatcc gcaaaaacac
     7921 gcccggaaac gagcaacaaa accaccagtg ttgaaaaaat cctcatactt ccaaatttac
     7981 acaaatctaa tcatttaatc tttaaaaaca aaaactaaat cgttaattaa aaaagcaagc
     8041 cccctatctt cacaacgcca aaagccacaa accatgcaac agcagtggtg tacaccaccg
     8101 taaacaaggc ccatttccaa cctccggttt cttttcggat ggcagcaaca accgccacac
     8161 atggaaaata aagcaataca aacattaaaa aggcaaatcc ggttacagga ttcaggacgg
     8221 gtttaccacc tgcatcagaa accaactgtt cgcccagtgt tttgccggca ggatgtttaa
     8281 tggcagaaat gcgtcctcct ttgtcattgt gttcatttga aaacaaaacc gacatggttg
     8341 agacaacaac ttcttttgct gcaacaccag ccaccagact aacggacaag cgccaatcaa
     8401 aaccaagcgg ttccatcacc ggctgaataa atttgccaat acgtccgata taactgtttt
     8461 cctgacgctc cgattcgatt tgctgaagaa caagctgatg ttcgcctgcc agcgccacgc
     8521 tgtctttttc gcccggccac acaacctctg tttccctgtt tttctgcatc tgcgcttcga
     8581 taagagctaa tttttcggac aactgaggtg aaggttcatt tttacgtgga aaatatccaa
     8641 gcgcccagat aagaacagat gcaatgagaa tgatcccacc catttttttc agatattgag
     8701 aagcacggaa ccacatatgg cgcaaaatag catgatgcgt aggcatccga tatggtggga
     8761 gttccatcac aaaaggcgct tcagttttac gaaaaatagt tttcttgaaa aacaaggaaa
     8821 acaaaacagc aaataaaacc ccaattgcat aaacaagaaa caacatccaa actgcatttt
     8881 gcggaaacag agccccgata acaaggatat ataccggtaa ccgtgcactg cacgacataa
     8941 atggatttat aagaatcgtc agcaggcgat cggtaggatt ttcaagggtg cgtgtagcca
     9001 taattgccgg gacattacac ccaaaaccca taatcatcgg aataaacgat tttccatgaa
     9061 gtccgattct atgcatgact ttatccatta aaaaagcaat acgggccata taaccggtat
     9121 cttccataat ggctatgaaa agaaacagaa taagaatatt gggcaaaaag accagcacac
     9181 caccaacccc tccaattatt ccatcgacca ttaaatcctt aaaccaacca tcgtgcatca
     9241 ccgcagcaat ctgctctccg atccatccca cccccaattc catccagtgc ataggaaacg
     9301 agcccacgaa aaaagtagaa aagaacatca gccagataaa aaacaaaaaa atgggaaaac
     9361 ccagaaaacg atgtgtaaga atgcgatcaa tgcgctgggt ggcagatgaa aaggaatcct
     9421 tacgcacacc cgaccgatat gtttccttga gggcaccggc aataaatcca tagcgtgcat
     9481 cagcaatcaa tgactctgta tcactatgat aaatggccga tattgtttca gattccttac
     9541 gggcaacatc atgcactttt tcaaaatcct tgcgggatgc agccgccagg gcctccctgt
     9601 cattttcaag caatttgatg gcataaaaac gcgatgaaac catttctttc aaaaaatgac
     9661 tctcgcacat ttgctcagaa attctttcaa tggcattttc aatttccatt acaaatcgga
     9721 tctccgcttt tcgaacatgc agactctgat tttcatacaa ttcaactact ttcgacaata
     9781 gctcatgaac ccctttgttt cgatgcccta ccgttggctg aaaaggaatc cccaacatct
     9841 caccaagact tttataatca aaataatcgc ctttggcaag caactcatca tgcatattca
     9901 atgctataac caccggaata tcaagttcca tcaattgtgt ggtcaaaaat aaattacgtt
     9961 ccagattgga agcgtctatc acgttgatga tgaaatccgg cggtgatgaa atcaattgct
    10021 tacgcacaaa caattcttca ggactgtagg ccgaaagact atatgttcct ggtaaatcaa
    10081 tcaggttgat tttataatcc ctgaaacgga atgaacccgt ttttgcttca acagtaaccc
    10141 cactgtaatt accgacatgc tccgtagaac ctgatgcaaa attaaaaaga gtggtctttc
    10201 cgcaattcgg attaccaaca atggccacgt ctatttcctt cgatctcaga atggctttgc
    10261 gctcaagcac ttcataggcc agattgctgc ttgttgttcc tgctgaattc atttcactct
    10321 cttcaaactg taacatttcc tccgttacaa cttcaatgga acgtgattcg ctcctgcgaa
    10381 gactgatttc ataatccatc aggcgatatt caaccggatc ctgcaatggg gctttacgta
    10441 cgacttctat cctccggcca ggagtaaaac ccatctcaat cacccgcttg cggaatgctc
    10501 cactgcctcg aacctttaca acatacccaa tctgaccggg gttcaaatcg tttaacgtca
    10561 ttatttagat ttaatttaaa taacattgca aaattaatga agttttttgt atcaacaata
    10621 ataaaatgat actttatgat ataaaacagg ggcaactttt aacaacaaaa aaacaagcgg
    10681 aatttccggt acggaaactt cgcctgttca agcacaaaac gtttttttac tgaatctgta
    10741 taaaagcaga gaatgaatcg ctctcgccga taaaacgaag accatacgtt ccttcactca
    10801 gcgaaccaac ttcaagcaat tcagactgta ccggattgtc aacaaccatg atggtctgac
    10861 ctgacattga tacaagctcg atgcgaataa ccgcgggtaa aatcatcgga tcgatttcga
    10921 tattcatgta ctcatcggcc ggatttggaa agactgaaat tttaccttca ttccaggcct
    10981 cttcaattcc agctaggcaa gtagtataga ataatttaaa cccatcggca cgcagcagat
    11041 tatcggactg gaaaacgata tgagcagagg gtccccagac aagaatttcg gatggcaact
    11101 caaaaccagt aacggatgtt accattggat cagatgtaga agttccgtta taaatataca
    11161 caacatccat atctttatcc aatgcgactt ccgtaaaatg aatatatatc aatcctgcat
    11221 cggcaggctg aatggtccat gaacaattgg tcccatttcc atatttattt actccgctgc
    11281 catcttcaat ggtatcggtc attgctgtat aaactgagga tggctgacaa tattcaacat
    11341 caccaatagc cggttctaca ccaaaaatag ctgcctgccc gtcattaaac cccagcatac
    11401 tgctgagcat aaaataacca ttggaagatc cgccccagcc ccagttgata tgaagtctgt
    11461 tgctgttgtc aattccatcg caaacaaacg catgaccgcc cgatgagcta tgtccgctat
    11521 aataaaccgg tcgcctgcaa cgtagttcat tgtcgataag ctgaatccag aattcatcag
    11581 acgtattgtc tctctcagca aaaaatgcgt aatcagcata tccaaaatga tccgcaaaag
    11641 cataggcggc atcctgcgaa ctggcaccag atccatcaaa atcatagccc atattcaccg
    11701 aaacgcctgc atgatacaac aattgagcta tgtatgaatt attggttgtt attgaattcg
    11761 gcattgcagc ccagttgtag gaggcctgac tgaaatcgac atgaatgttt gaataattac
    11821 ctgttccata aatattagaa cctgtaccca ccggtggata gttgtgatat ttcatcacct
    11881 gacccatggc cgtggccaca caaccagcat aacaatgacc acagggacca gttgcagctt
    11941 caggcacact gtcattgtaa taacaatcct gattccatgt agtggataga agaggtgtta
    12001 cggccgattt cagatacacc ggcagctcgc catacttcga caatgatttc cattcggcct
    12061 ctaccaaagc atcgggaacc gctttttcgc tgattgcttt ttcaattccg gcattcacgc
    12121 ctatgtaata atccatgacc ggcgccggca attgcgcatc cagacgtgat tcaaaggaat
    12181 aggcaataac aggggttact gttttttcac tggagattac aacaaaagaa gtattgcttt
    12241 catcggtgaa ataatacagg tgattcatac cttttgtttc aactgacacg ggcttgagtg
    12301 agatagaccg tgatgctttt gacgtcataa aattctgagc aagggtcaat gcttcggatt
    12361 gactaacttt ttgcgccatg gatgcaaatg caaacagcat taaaacaaaa gaagaaagaa
    12421 ttctcatggg taaggatttt gtacaaaaat atgcaatctt tgcattagac aataaaaatt
    12481 atttattgtt gcaaggccat aaaaatcagt cgcttacata atccgcaatt caataaacca
    12541 atgttctaaa acaaagctga cagcgacaat aagccactat ttatcctgta aaaaccgtaa
    12601 actgatttaa acaacaccaa aactgattgg tcatgatgat gcagattgta agtttcagaa
    12661 atcaatatcc agcaccgacg gacaatgatc gctgtgcatt gcatctggcc agatttcggc
    12721 actttttagt tttgagcgga gcgattcggt agccatgata taatcaattc tccatccctt
    12781 attattagcg cgggcattgg cgcggaaact ccaccaggtg tagttgtgcg gctctttgtt
    12841 gaaatgccgg aaactgtcga tgtattcgtg acttaaaaat ttcgtaagcc actcacgctc
    12901 ttcgggcagg aagccggaac tctttgcatt gccgaccgga tcatggatgt caatcttttc
    12961 gtgtgcaata ttgtaatcgc cgcacaaaag cagattgggc ctttctttgc gcaattcctg
    13021 cacataatcg agatagcggt cgagccatgt cattttgaat gcctgtcgct catcaccaga
    13081 agtgcccgat ggatgatagg cagagacaat gctcaagtcg ccgaaatcag cacggataaa
    13141 acggccttcg taatcatatt cggcaatgcc cataccagca acaactttgt ccggctgtat
    13201 gcgtgtgatt attccgacgc cgctgtagcc tttcttctgc gcactgtaaa aataagtttt
    13261 atacccgagt gcttcgaatt ccatgaccgg aatctgatcg ggctgtgctt tggtttcctg
    13321 tacacacacc acatcggcat cgctctgcgt gagccattcg agaaatccct taccgatggc
    13381 ggcgcggatt ccgttgagat tataagtgac aactttcata tttatttttg tggtaataat
    13441 ttttccaatt cagtctgatc cgtaattttc aataccggat atacacaata cagctgatcg
    13501 cgatacaccg tatgattata cacccagttc atctgcagcc attgattgga tgcaaataca
    13561 gaatcgcttt tcttcaatgc ttcaaactcc gctttcagtg ccggattttc atcaaacatt
    13621 ttttcagcca tgggttcaag cacatacatt tcgccatatt ccttctgctc gaaaattgca
    13681 ttgaaaaagc cccatttcag gaaactgtct tccgaagcgg catcgagcaa ccaaaccaat
    13741 acttttattt ttggctgttt taccaaaaca agagccgatc cttttggaat attcattgtc
    13801 gtatcaaatt cctgaatgtt gaaggacggc tgaaaacgcc cttcaaaaca ggtcttcggg
    13861 aactttacat tgtttagttt tgatacggtt acatgcaatg caacatcttt agatgatgta
    13921 ataacactga ttccatggag ctttacacgt tcaatcacat ccacccattc tacaggaatc
    13981 acataggcca gcggtagttt tatgctgtca ctcacataca ctttatcaaa caaaggaatt
    14041 ttccatgtaa ccggttttgt attgtcatat ttgtaataaa tgccgcctgt gatgccgctt
    14101 ttcagggtgt caaactgcat tccgagaaag tctataatgg tgctatcatt catatccgtt
    14161 gcaagtgcta ttggaaaagg tgacagggca aacttatcag aaacgcgctc ctgatcagcc
    14221 ccacgaagta ttgacatata gctttcatga ttatcggata aatatttaaa tgtgtgaaac
    14281 aacatgtttt ttgttgcatt cacacgtacc ttatatggct tcagcatatg tgtttcaatg
    14341 agcaaacacg gacgatccga aaggatggta taaccatgcg aaagcatcgg gcgcgagcca
    14401 tacgtaataa gtccgctgcg aggattgtgc cattggcgga aagccacata aggaaatata
    14461 ggcatgccat cgcgattcat tttttccgaa acaaaaggaa tatatttttt agtacaccat
    14521 gcagccactg taggattcag tgtttgaaaa gcatccagcc cataagtcag cggatattga
    14581 taatcggcac catcggtagt atgacaatca atcaaaaaat gaggattaat ttcattatac
    14641 aatttcagcc atgcctgcat ttcaggcgca tcagctttca tgaaatcacg attaagatta
    14701 agattctgag catttgttcg ccagcccatc tgctccggac cgttttggtt aatgcggtta
    14761 tacggaccaa aacgcttcaa tccatccaca ttaaagcatg gaatgaaaag aatgcgaaca
    14821 ttccgaagca tcggtctcat ttcaggtttc gtcatcaggt cgcgcagaaa aagaaatccg
    14881 gcttcaacgc cttcgggctc tcctggatga attccggcct gtatccacaa ggtgatttta
    14941 tcggttgtct tcgaaccatc atctatcata aggtatggca aatcatctcc ctgagacgat
    15001 tttccgaatg atttaaaaga aacttcgctg tacaacgaat ctgtcagacg acaataatga
    15061 ataatttcat catacgtcat tgtcacattc tgacctttcg attcataagg gatggagaag
    15121 gttttcgttt gcgcttcaac atgtgaggca aacaaaaaaa acaagggaat aattaagaca
    15181 gttctcatat aattcgtttt cattttctta atgtatcgat tccatctata tccatgttag
    15241 cacttgtatt attgactgtt acaacaatac tcagaaatat attcatgcca ccagagccac
    15301 attcaaccat attcctgacg tcaggaaaat ggtctgcgaa tgtctgggac gctgttttca
    15361 gagatcgcat ttttttgaca atttgaattt ttatcaacat cggaacacaa aatttattga
    15421 acacctttca tactcagatt tattcttttt ctcggcttat caacctcgag aatacgaact
    15481 tttactttct gattaagctt cagcacttcg gaaggatccg caataaagcg atctgcaatc
    15541 tgactgatgt gaaccagtcc atcctgatgc acaccgatat cgacaaaagc accgaattta
    15601 gtaatgttgg tcacaatgcc gggcaattcc atgcctgcaa tcaaatcgtc gatgctgtga
    15661 acattctgat caaattcaaa tatttcaaat ttctcacgcg ggtcgcggcc tggcttttcg
    15721 agctctttca gaatatcatt gatggttggc aatccaaatt tttctgtaat ataattctga
    15781 ggttttattt gtgcacgaag ctctttattc tgaattaaat cgttaacggt acaattcata
    15841 tccttcgcca ttttctcaac aatgccatag ctctcggggt gtacagccga ggcgtcaagc
    15901 ggatttgcag catccttgat gcgcaggaaa cctgcggcct gttcaaatgc tttgtcaccg
    15961 aaacgcttta ctttcagcaa atcttttcgt gttttaaaag ctcctttttg ctttctgtgt
    16021 tcaacaatgt tctgcgccat cgcagggcca atgcccgaaa cgtaggtcag gagctgtttg
    16081 ctggcggtat tgagttcaac gcccacatta ttcaccgcac tcataacagt ttcatccagc
    16141 ttttccttca gcatattctg atctacatcg tgctgatact gtcccactcc gatgcttttc
    16201 gcatcaattt tcaccagctc tgcaagtggg tccattagtc gccgtccgat agaaacagcg
    16261 ccgcgcactg taatgtcata atccggaaat tcttcgcggg cgacatcgct ggcggaataa
    16321 atcgaagccc cggattcgtt gactgcaagc gcaatgacag gtctgctgaa atgaatgcgt
    16381 ttaatgaaag cttctgtttc gcggccagca gtgccattac caatggcaat ggcctcaatt
    16441 ttataagctt caacaagatg atcaatttta ttcaacgcct ctttcacctt actttccggc
    16501 ggatgcggat aaatggtttc gttatgcagc agatttcctt gcggatcaag cactaccact
    16561 ttgcatccgg tacgaaatcc aggatcaatg gcgagcacca tcttctgtcc aagcggtgat
    16621 gcaagcagca gctggcgcac attgtcggca aaaactgcaa tggcttcctt atcggctttt
    16681 tctttgtaaa atttccttac ttcagtttca agtgacggct gcaacaagcg gttataacta
    16741 tcgtccaccg ctttctgcac ttcttcggca atggcgtttt ttgctttcac aatatgccgg
    16801 tcaatcagtc ccagagcccg ttcttcgtcc ggctcaactg atacgcgcag aaatccttca
    16861 ttctctccgc gaaatatggc aagaatcctg tgcgaaggcg cttttgccaa gggttcggaa
    16921 atggagaaat aattttcaaa cttggcacct tcggtttctt ttcccttagc cactttggaa
    16981 taaatcactc cttcgcggat aaagagtgcg cgcagatcgg ctctgacacg ggcatcttcg
    17041 ctgatccatt ctgcaatgat gtcgcgtgca ccggcaatgg cttcctcttc gctaaccacc
    17101 cctttttctt cgctgataaa atgccgggca gcttccataa tatcaaacgg ttgctggtca
    17161 aaaatacgct ttgccagggg ttccagtcct tttgcaatgg caacggtcgc acgcgttttc
    17221 cgttttggtt tgtaagggag atacaagtcc tcgacctcag gcattgtcat cgcatttttg
    17281 attttatctt caagttcggg tgtcaatttt ccctgctcgc tgatgctttc aagaacagcc
    17341 gctttgcgct tctcaagctc cgttaactga tgcagacggg ttttaatggc aagaatctga
    17401 acttcatcca atgtcccggt catctcctta cggtaacggg caatgaaagg aaccgtagct
    17461 ccttcatcaa gaagtttaat ggtgttggca acctgaacgg gtttcaggcc cagctcaatg
    17521 caaataatgt cggtgaaaga tttcatgttt caaaaatagc aattttcgct caatagtaag
    17581 atgtagtgga ataataaatc atgaacaaga aaatatgagt caatttcatt ttggctatat
    17641 atccatcttt ttttattgtt atagccattt tgaattttat attttgcttt tactatgaat
    17701 atttatatat ttgcagccaa aataaaatta tatgtgcgac atcgttggca gagatgaaga
    17761 aatagaaatc atgcgcgatc ttgagcagtc gcgcgtttct gaatttttag tcgtatatgg
    17821 gcgccggcgc attggtaaaa catatctgat ccgggaagta tttcagaata agtttgtctt
    17881 ttcaattacc ggaattgaca atgtaaatat gacacagcag ctgatgaatt tcaatcttac
    17941 gctcaggcag cacgatgctg acggggaatg ggagcccgtc aactgctggc tgctggcatt
    18001 taatcaattg cgaaagtttc tggaaaagtc aaaaaaaaag cgaaaaatag tattttttga
    18061 tgaactaccc tggttcgaca cgcagcattc cgacttcatt caggggctgg agcatttctg
    18121 gaacagctgg gcatcaggac gtaaggatgt tttcctgatt gttagcggtt cggctacatc
    18181 atggatgatc aacaagctca tcaataaccg tggtggtttg cacaatcgcg tcacccagcg
    18241 catctgtctg gaaccattta cactggccga atgcagcgag tttatgaaaa ggaaaaatgt
    18301 ccatcttgat cagtatcagc ttattcagtt atatatggtg atgggcggag taccatacta
    18361 ttggaataaa gtgaccaggg gaaaaagcgc ggcacagatt attgatcgcg aatgcttcag
    18421 ccgcaacggc tcgttgaaag acgaattcaa taatattttc aaatcgcttt tccgtcatca
    18481 ccagaggcat ataagcatag taaaagctct ggccaccaaa tcgaaaggac tcaaccgcaa
    18541 caaaatttca aaattgtcgg gtatatcgaa tggcggcggc ctgacacgat tgctggatga
    18601 actggaggcc tgtcatttca taacccgcta cattccattt ggccgtacaa gccgcgacag
    18661 tctgtatcag ctgaatgaca tgttcagtct gtttcatctc agattcatgc atacatcaac
    18721 aaaaaaagat gcaggcaact ggctgaaaac cattgatagt ccgaagcacc gggcatggtc
    18781 gggatatgca ttcgaacagg tttgcatgca gcacagtgcg caaataaaaa aagctttggg
    18841 tatatcgggc attgaaacac atatttcgtc atggcgcagc aggagcgatg attcgggggc
    18901 gcaggccgat atgatcatcg atcgccgcga ccagattatc aatttgtgtg agatgaaata
    18961 ttcagtgaac ccgtacatca tcaataaaaa atacgcgaca gaactcagtg agaaagcagg
    19021 acgtttcagg tacgacacga aaacaaaaaa agccgtacat attgttctgc tcacaacatt
    19081 tggagttgca aacatggagc attacggcgg cattattcag cataacctta caatggattg
    19141 tctgtttgaa aaagcataaa agaaccagct tcaatatgaa aacattcttt tcaaagaacc
    19201 gaaaatatta tcttcgcaac gaaataacaa gagcttatga gtgaccagct gaagaaaaaa
    19261 aggcagatac caagtacata cgttattgtt tttacgataa tccttattgc tgcagcagcc
    19321 acctggctgg ttccgggcgg ttcattcgag cgcgagaaag tgatggtgaa cggcgtcgaa
    19381 cgcgacattg ttgttcaggg atcatatcac gaaaccgaga ctaatcctca aagttgggaa
    19441 atacttgctt ccgtttatga cggatttatt gataaggccg acatcattgt ttttattctg
    19501 gtaatcggag cttcgttctg gattatcaat tcatcaaagg cgctcgacat tggaatcatc
    19561 aagtttctga gtcgcgccga gcgacttgaa aaatataaga tcttcagaaa aatcggcatc
    19621 cggaatattc tgattacgct gattatgctc atgttttcag ttttcggagc cgtatttggc
    19681 atgagtgaag aaaccattgc ctttgtgatt attttcgtac cgatggctat taaaatgggt
    19741 tacgacagca ttgtaggtgt cagcatgtgt tttgtcggag cagcaatggg tttcgcaggt
    19801 gcattattta acccattcac cattggaatt gcacagggca ttgcacagat tccattattc
    19861 agcggacttg aataccgttt atttatgtgg attgtattga atattttcgg aatttcctat
    19921 atactgattt acgccaacaa agtatataaa aatccgcaac gatctgtggt gtacgaaacc
    19981 gatcagtact ggcgcgatca ggcaacaaat aacgaacatc cggtggagac caaagccggt
    20041 atttcagcct gggtttcgtt tggaattatt ttgttttcgc tgattgcatt ctggacgaaa
    20101 tatccctaca ccgatattac ggttggttct tttactgtta ccatcccggc gctgccaatt
    20161 ttgactttcc tgtttgctat tcccggaata tggcttctgg tgcggaaaag cgtacaactg
    20221 tacattttgc atatcatgtt gttcaccacc atttcgctcg taatcggtgt acttggattt
    20281 cactggtata tccgcgaaat tgcagcgctg tttttcggaa tgggcctcat gggcggcata
    20341 gcggcgaatt atcgtccgaa tgaattcacc aaacatttta tggacggcgc aaaagatatt
    20401 ttttcagccg cactgattgt tggtctggct ggcggcatca ttattatcct gcaggaaggc
    20461 ggcattgttg atacgctctt aaattcgctc tcctcattgt tttcgcacaa taaatattct
    20521 gcagtcagtg gcatgtatgt agttcaaacc gctataaatc tcattatgcc atctggctca
    20581 gccaaagctg cgctcaccat gccactgatg gcgccatttt ccgatcttat cggaatttca
    20641 cgccaggcct cggtaattgc atttcagctg ggagacggta ttacaaacat gatcacgccg
    20701 gtttcagggg tgctgctggg cgttttaagc atggcaaaaa tcccttatga gcgctggttc
    20761 aaatgggcct ggaaatttat tctgatgctg attattcttg gatttttgtt actgattccg
    20821 acggttacaa tgaccttgaa cggattctaa aatacgaaat cagaaatacg aacgaaaaca
    20881 attcaagaaa acaaaataga atttcacaaa tcgtatttcg tatctcgtat ttcgatctcg
    20941 tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatctcgt atttcgtatt
    21061 tcgtatctcg catctcgtat ttcgtatttc gtaaatcaaa acgctccttc atcgtcgccg
    21121 caggcgcgct ttttagcttc gacggaaccg tcgtatttga gatctacggc tttctgtaaa
    21181 tcttcgcagg cacctttttt atcgccgttc atgtcgcgga tagctccccg gtaaaaatat
    21241 gcacggcctg attccgggct gtatttgatg gccttatcca aatcttcggt ggcagcagtg
    21301 aaatttttct ttttgaaata atacacgccg cggccttccc atgcatagcc gtttttcgga
    21361 ttgatcgtaa tgattttgtt atagtctgtc agcgccgatg cctccttgtt tgtggccact
    21421 ttgctgtcgg cgcggttgat ataggcatct tcgaaatccc tgttatacat aacggccttg
    21481 ttatagtccg caatagcaag taaatgctga ttggtagcag cataacaaag cccacgggca
    21541 ttccacaacg aagcatcttt cggagcatag gttgtcaata aaaccgtcag atccgaaatg
    21601 gcggtctcat attgtttggc atccatgcac agtttgtagc ggcggcgata cacatcttcg
    21661 tttacaaatt tattgttgat ggcagcagta taatcttcaa tagcgacctg aaaacgttca
    21721 accttctcaa gcaaaacacc gcgccaataa aaggcttcgc cataagtttt gtttttgtca
    21781 atcactttcg agaaatcgtt gaacgcttgt tcctgcatgt ctttgttgaa atatattttc
    21841 ccgcgcgaca ccagcgtagc cggatcatcg ggtcggattt caagaatctt gttataatcg
    21901 gccaatgcag catcgttgtt attctgctcg gcataccagg ctgcacgcag gatgtacgga
    21961 tccgccgatt tcggtcgaat gttcactgcc tgtgtaatat cggccaacgc ttctttcatt
    22021 tgctttgcat ctttgtacca gagcgctctg taatagaatg caaaatagaa atccttcttt
    22081 tttgtgagcg ccagattgta atcaatcagc gccagatcct tctttccaag tgcaaactga
    22141 caacgaccac gatcaacata ggcctcgtaa taatacggac tgatttcaat gcatttggtg
    22201 aaaagcggga tggcacctgc gtaatccttt gcattcagtt tggccgtacc ggcatcgtaa
    22261 taatcgttgg cgctctgctg ggcaaacagc gaaaacgaat aaataaaagc agcaacaaaa
    22321 agaaaatgtt tcatcgtgag aaattgatat gcgaaaataa aaaaatactt tgtaataatg
    22381 acctgagatt attctaatca gcttttcgca tacaagcaac cgcaattatc caagaaaaaa
    22441 agtcatttaa aaatccaata ctgatgcaac tcaccaatac ttctatgttt acgaagccac
    22501 tgcaaacaat ggaatattga gttaaaaact acttgcaaaa ttttaaaatc attcaggtct
    22561 tccgacaatc aaaatattca ttgagcgcac accgtatggc agatctgtga tgccggtgcc
    22621 agaaatatcc atccagcgca gttgtgtgag ttcatcgaaa aaggaaggta aactgatgat
    22681 gtttgtatgt gcaatactaa gactttcgag attcttcagc tcattgaaaa atgaagggaa
    22741 gctgctgact ttggtataac tcatatcaag cacacgcaaa gacttcaatt cataaaaaaa
    22801 tgatgcaaac gaagtaatgg gatttcctga taaatcgagt atttcaagat ttttcaattc
    22861 actgaaataa gacggaaatg aagtaaaatt attattctcg gcctcgatta cacgcaactg
    22921 tcgcagatcg ctgaaaaaag aaggccatga actgaaatta ttattactga tataaagttc
    22981 gcgcaaattt tccatatcac taaaaaacga gggccacgta ctcagattct gacctctgac
    23041 atcaagcaca ataacgtctt cgggattcct tgcggccgct tcaatattat aataaacacc
    23101 agaattctgc ggtatataaa cattttgcac ctgcggctcg atataattat tctgcacatc
    23161 attattgtaa ttctcagaat tatcgtaatt gtaattattg ttgttataat tgttgctgtt
    23221 gttatatgaa tccgatgtat tgtaattatt gctttgagga attggaagac cgctttgggc
    23281 atcacgcatg acaaggatgg agctgatgtt gttgttccat ggcgcgaaat cgccagtggc
    23341 ggtccagtca ttttcaatca ccagataatg gccacggaac ccagattcat caaagatgac
    23401 aatccatgtt cccgtaggga tcctaattga gccgatgcgg ttaataaaaa catctgacag
    23461 gccttcgggg ttataatggc tgtacaaatt gcgcgagtca cccgtaaaat tttcattatc
    23521 atatactgta acagcgtcgt tttgagctat gcaggttacg gaaaacaggg cgaacaaaat
    23581 aagtgtggcg aagtgtttca tggtggctgc ggtttgttgg tgtgtgccaa gttaggaaac
    23641 tttgcacagc tgtgatgtta aaaaatgagc aaagggcttg caaaattcga gccagagggt
    23701 agagtgcgaa gttggaaact tcgcacagtg ctgtatggaa ttcgcacagc gcttacttga
    23761 aaacagcttt ataggctgct tttagcatga acagacattt caaatagtgt gtttagcatc
    23821 catcaaattc attgaatata gtcaatgttt tagtcatatt tgatattttc attgagtata
    23881 gtcaataaat tttgcaaata acatgaaagg tttcagtata gtcaatactt ttctctgaca
    23941 cacaaaatga ttaagacaat ataaataaaa tattatcttt gaagacgaaa acaataaaac
    24001 aatataataa ttgtatcatg agaaaaatta aagtgtgcct gtatttcact tttctactgc
    24061 tgcctgcaat cagtttttgt cagacaaatt atttggaagc gggtatcagc ggcggttccg
    24121 gaatacgtat tctgagtgga gaatacattg ataataaata tactgatcct gccatcggtt
    24181 atcacttcgg tggaactatt cagtatcatt ttggaaaaat cttttcgctc aggactgacc
    24241 cttctttcga acagaaagga aatacagatt atctcatact gactgataat gtcggtaatg
    24301 aaattggcca tctggatgcc aaaaacaatt taaattatat tgtattgcct gttctgggac
    24361 aattaagttt agggaacagg attgtattta ctatacacgc tggcgctttc gcaggatact
    24421 taatcagaaa caaccatata atgcccgcat ttgaaactta cgctgaacag aaaataaata
    24481 atacagcttc tcataaaaga tttgatattg gacttgcagg tggattcgga tgccaatttc
    24541 cattgaaaga gaatttttca atattcatgg atgcccgggt aaatgctggc atttttgata
    24601 ttcaaaagtc acccctaaca ggacccgttt acacaaattc attgaatgta actttaggaa
    24661 taaagtacaa attcactaag cggacagacc ttaaagagtc tgcaattaac tgaatttcac
    24721 cgcagcatac tgcgcgaacc ggatgttgtg gctgaggagg ggtgcgacgt tggaaacgtc
    24781 gctcagatgt ggggtacgcg gaattgaaaa actccgcgca gcgtagcaaa acgattattt
    24841 gctaaaaaca gcttcagcaa ctgcaatggc ttcgttcaaa ccctctttgt tggtgccgcc
    24901 ggctgaggcc aggaatggct gaccaccgcc gccgccgttg attggtttgg caatttcgcg
    24961 gatcagctgt ccggcattcc atcctttgtc tttcaccagc ttatcgctga tcagcagcgt
    25021 gattccggct ttctcgccat tggccgagca aagcacagcc accagattgt cgctcagggt
    25081 gcggagtcca aaggcaatat ccttcagatc gccggtaggt gcgtccagta ttttcccgag
    25141 aaatttaatt ccgcccatat ctttggcttc agccagcaga gcgggcgcaa gcgacacgag
    25201 cttttccttt ttcaatgtct caatttcttt ctgcagtcct gaaagttttt cgttcagttc
    25261 agtgactgca acaacaggat ctttcccttt gaaaatctct ttcagtccgc gaagctgttc
    25321 atccatcttt tcaatcatgg attcagcaac agaacctgac acagcttcga tacgacgtac
    25381 accggctgca atggcgctct cagagacaat tttgaaatat ccaatcattc ctgttgaagg
    25441 gatatgtgtt cctccgcaaa gctcaacact gcttccgaag cgaattacac gaaccttatc
    25501 gccatacttt tcaccgaaca aagccatggc acccattgcc aaagcctctt cctgcttcat
    25561 ttcgcgatac tcatcgagtg aaatattttc gcggattttt tcattcacca tgcgttccac
    25621 tttcagtaat tcttcgttgc tgagtttttg aaaatgcgaa aaatcgaaac gcagatattc
    25681 tggcgaaacc aaagatcctt tttgttcgac atgtgtacca agaaccgcgc gcagtgcttc
    25741 gtgcaacagg tgggttgccg aatgattgcg catggtgtcc atgcggtttt cagcattcac
    25801 ggccgctatg aaaatggctt tcgggtcttc gggcaatgtg tcggttacat gaatgatcaa
    25861 tcgattttct ttgatggtat tgatgatttc aatttcatct ttttcggatt gtagtaaacc
    25921 gctatcgccc acctgacctc cgctttcggc atagaaaggt gtcttgtcga gtactatctg
    25981 aaaaatcgtt tttccttttt gttccaccga acgatagcgc gcaatgcgga cttcgtcggt
    26041 caaatgatcg tagccggtga attcggtaac gacatccgga atgatttcaa cccagtcgcc
    26101 ggcggccaca gcagcgtctt tgcggcttcg gttcttctgc tctgagagat aagtattgaa
    26161 ttcatcaatc tgaacatcaa ggttgcgttc acgcgccatg agcaaggtca ggtcaatcgg
    26221 gaagccgtag gtatcataca attcgaatgc aaatttccca tcaatggttt tacttcctgc
    26281 cagctttgat acatagtttt cgaacatatg aacaccggtt gccaaggtgc gcagaaatga
    26341 attttcctct tcgcggatca catttccgat gagttccttt tgtttgacaa gttctgtaaa
    26401 ctgagttccc atctgttttt cgagcacacc cagcaattta tgcataaacg gttcggtaaa
    26461 accaaggaat gaataaccat agcgtaccgc gcgacgaaga atgcggcgga taacataacc
    26521 ggccttagca ttcgaaggca gctgaccatc tgcaatggca aagcttacag cccgcatatg
    26581 atcggcgatt acgcgcatag caatatcgtt cttttcatcc ttgccatatg ggattccgct
    26641 caaagagctg atttcagcaa tcaatggcgt gaagacatcg gtatcgtaat tggatgtttt
    26701 attctgaatg gcacgcacca ggcgttcgaa acccataccg gtatcgacat gcttggctgg
    26761 cagtggttcg agttttccgg aggcattgcg attatattga ataaaaacca ggttccagat
    26821 ttcaatcacc tgaggatgat cgttgttgac cagcgtcttt ccatccacgg aagcacgttc
    26881 ggcatcggta cggaggtcaa catgaatttc ggagcaggga ccacaggggc cggtatcgcc
    26941 catttcccaa aaattgtctt tcttggaacc aggtaatatg cggtctttgg caatatgtgt
    27001 tttccaaaaa tcagcggctt cggtgtcagg atcaagcttt tcatgctcat cgcctccgaa
    27061 ataagtaaca aacaaacggt ctttgtcgag tttaaaaacg tcagtcagta gttcccatgc
    27121 ccactcaatg gcttctttct tgaaataatc gccaaacgac cagttaccga gcatttcgaa
    27181 catagtatgg tgataagtat cgatgcccac ttcttccagg tcattgtgtt tccccgatac
    27241 gcgtaaacat ttctggctat ctgccactcg tttgcttttc gggatttcat ttccgagaaa
    27301 atagtcctta aactgattca ttccggcatt ggtgaacatc aatgttgggt catttttcaa
    27361 caccataggc gctgaaggca cgatggaatg cgactttgaa gcgaaaaagt ctaaaaaggc
    27421 cttacgaatt tcgttgctgg tcataaatat cttgttcagt agtatttgtg aaatcggcta
    27481 caaaattagt ttatttatta attttgcata catttcagaa tatgtccgat caacatttgc
    27541 gtttcaatcc acaaacaatg cactacgagg tagtgtataa aaaacactcg cgctggcgta
    27601 atgttttgta tattttactg gttggttcag catttggtct tgtcttcatt tttttgggaa
    27661 caacatttat aaaatcaaag gaagtcagaa aaaaagagca tgatcttgcc gtattcaaag
    27721 agacttacga aaaagcagag cttcaatatc aacaaaacat cgagttgctg cataatcttg
    27781 agatgcaaga ccggcgcctt tttcgggaga tatttgagac cgatccggct gacacgacct
    27841 tactcagcaa tcaattgctc gaagaagcac aaatcgactc agaaaacacc gatatctctg
    27901 gcatgttttc aatgatcaaa gcacaataca aaaaaataga aaacatctcg tacaaagatg
    27961 ccatccgttt acaatttgcc agttattttt taaaaaacaa ttcagactat atcaaatcgc
    28021 tgccggtacg tgttccatta aaaagcggca gctacacact ggtttcagga tttgggaaac
    28081 gcatacatcc cattttcaaa acagcacgtc agcacaatgg gctcgacctt gcagcacgtg
    28141 caggctctga ggttctggct tcaggcgacg gaattgtgca ggatgttcca aaaaatctgg
    28201 caggatacgg taatgtggtt tatatctacc acgggcacgg atatcatagt ctttatgcac
    28261 aattactgga gtcaagggta aaacccggac aaaaaataca tgcaggtgat gttattggat
    28321 ttgtaggcag caccggcatt tcgatcgggc cacaccttca ttatgaaata tggaaaaacg
    28381 gcaaacccgt tgatcctatc cgctttctgt tgaccatcgc tccaggcgaa tatctcgaaa
    28441 tgtacaaggt ggccactcaa tacaatcaat gcctctcctg atgaacaaag aactcaaaaa
    28501 actcacactt tttactgcca ttaattttgt cattgccaac atggtaggga caggcgtttt
    28561 cacctcactc ggttttcagc ttttcgatat taaaaatccg gcagccattc tgattttatg
    28621 ggctatcggt ggcattatgg cattatttgg ttcttttgtt tacggtgagc ttggcgcagc
    28681 catgccccgc agtggtggtg aatataattt tctaagtcgc atttatcatc cctcgcttgg
    28741 ttttttggcc ggctggattt cggccaccat tggatttgcc gcaccggtag cagcggcctg
    28801 catagctttt gggacttatt atcatcagtc aattgatata ttaagcaaca cctattctgt
    28861 caactggctt caaacaactc ctatgattgc cggactgatt attctggccc ttattacaat
    28921 ggtgcacagc aattccatta aaacaggcgg cagattccaa aatgtattca ccattctgaa
    28981 acttttattc atagttattt tcataggagc aggattgttt tttctgccag aatcgcaaac
    29041 tctttctttc gctttcagcg ctgatacagg caatgacatt ctaaaccccg cctttgccgt
    29101 tgcattggta tttgtgagct atgcctacag cggatggaat gcctcggcct attttgtcaa
    29161 tgatctgaaa aatccggtaa aacaattgcc ccgctcgctc atcatcggaa gtattgctgt
    29221 gatgattctt tacatattgt tgaattttgt ttttctcatt tccaccccga ttgaagaaat
    29281 gattggtaat cctgagatag gacttatttc agcaatgtat atattcggca acaaaatggg
    29341 tgttttcatg ggacttttta tttcattatt gcttgtctca agcatcagtt cgatgatatt
    29401 tgcaggtccg cgtgttacca tggttatggg agaagatttc agtttattga agtttctggc
    29461 gagaaaaaac aaaaacgaaa ttccttacat cgcgctcatt acgcaattta tcatcagttt
    29521 cacccttata attacatcga cttttgaatc actgataaca tacactggat ttgtactgaa
    29581 cctgttcaca tttttcacag tactgggtgt tttcgtacat agaaaaaaat tcccggatgc
    29641 agatcgacca gtgaagacct ggggctaccc ggttacgccc attatttttc tgattttcaa
    29701 tctctggatc actattttcg tgatatactc gcgaccgctg gagtcgttac tgggtctggc
    29761 aactatagct gtcggcgcaa ttctgtattt tttcagcaaa caatcaaaca aacccaaaac
    29821 catatgaaga caagcctttt cattctgatg ctcagcatct tttttatgat ttcatgcagc
    29881 ggcagcagca aagacaaaca aaacggcgca gactccacca caactgacac tgtaacaaag
    29941 accgaagcca tcgtcttaaa tcactactat aacgacattg cgcgctttgt agcaggaatg
    30001 cccctgagcg acagcagtct gctgaagaaa tacaccgaaa aaaccgaatg gaaaaactat
    30061 tcagccggta ttgatagcag ctgggcaaat tttcagaaaa ccaagctgga tgtcatgcaa
    30121 ccctggatca acaaagaact gaatgcaatc aatgaaaaaa caaaggatgt tttttatcca
    30181 ttcagcggcc ccgacttcgt ttacatgtat cattttctgc caaaagcaga caactattat
    30241 atggttgcat tggagccggt tggaatcatt ccggacataa cgaaaattga aaattccatg
    30301 ccttcgtttt tcgcagcact gaacaacgct atcaaagaca atctgaacct aagcttcttt
    30361 ataaccaaaa gcatgaaagt gcaaatgaac aacgaacaga tcaaaggaac cattcctgtg
    30421 ttgctgtttt tcatggcccg aatgaatctg cacattcaaa acatcattcc tgcaaccata
    30481 gccaaagatg gaaaactatc ggttaatgag cagattgctg aaaacagcgc caataagaaa
    30541 tttacgaatg gcgtggaaat cagctttgta cgcccgggag agaacaaagt caacaaactt
    30601 tattatttca gcgtaagtat ccgcaacgac ggatttgcga gcatgcccga agccgaaaca
    30661 tatctgaaat cgctacccgc cgacatgaca acaattgtta aatcaagtag ttattgcatg
    30721 catgaagaca aatataataa aatccgcgac attgttctga cgcattccaa atttttgatt
    30781 caggatgaca gcggagttcc tttcagattt tttaacaaag aaaaatggaa tttcggttat
    30841 tatggtattt atacccgacc aattcctgtt ttttcacatt tttatcagga tgaacttaaa
    30901 aaaacatggc cgcaaaacgc cacaaaattg agttttcgtt ttggatataa cgaagaatcc
    30961 aatatctttg tagctacaag aaaaaattaa cggatgagta aaacaaagat tacaaccata
    31021 cttattgcat tttcaggcat cattttattg tctctgccat catcaggaca atttgattca
    31081 accaagatta tagacaccat tgaatcaaac ggaaaacaga ttgtgctttt cagcgataac
    31141 gtctggtcgt acatcagctc aatggaagat cttgcgcacg acaaagccat agccgacaca
    31201 gggcgggttt ttaccgagta ttggaatgat ctgacttttg cctacatgta tcccgaagac
    31261 aaaccactgc ccgatactgt tcagattgtg cttatagata gtcttcattc attcactcca
    31321 cctttattgg gcggcattac atctggcttt ggatggcgtt cggggcgcgt tcataaagct
    31381 gttgatatcg gccttgattt tggcacccct atccgatcag cttttgatgg caaagttcgt
    31441 tatgcacaat acaacaccgg tggctatggc aatctgatta taatcaggca tttcaatggt
    31501 cttgaaacct attatgccca cctatcaaaa atgtatgtgc gacctggcca aatggtgaaa
    31561 gccggacagg taattgctgg cggcggtaat acaggggcac attggtccgg tccgcatctg
    31621 cacttcgagt gccgctatcg tgaccacgct tttgacccgc tcaaaattat gcgattcgac
    31681 tcaacagaat taaaatcgga tacactcatt cttactgccg cagattttaa agtctcccaa
    31741 aatcacaaag catatctcgc cgatggagta aacggaacca ataccggaac atcttactca
    31801 aataccagac aatatggggg aagtacttcg gacaacagcg actataaagg atcctcagcc
    31861 aacggaacag ccataggcaa ttattacatt gtcaaaaagg gcgatacttt gtcagccatt
    31921 gcacgaaaat atggaacaac cgttgataaa ctctgcaaaa tcaatggtat caataagaat
    31981 gctattctga gactgggtca gaaaatcagg gtcagataat gttcggcaga tttttcattt
    32041 ttgttttctg cctgttaggg gttactgttg catattcaca agactggcca aaggaaagag
    32101 atacctcgtg tttgcccggc aaaatcatct ttgaacaata ctgcgattcg tttccggaaa
    32161 tctacatgat gcccgaatgg tacaagctgc agctgatcta tgtgcaggtt aaccgcgaca
    32221 aaaacggaat tcctcatctg acgtatcaca aactcggtgt tgaccgcaac aaatattttt
    32281 atccggccag tcttgtcaag ctccctactc tgcttctaac cctcgagaag cttcaatcgc
    32341 tcaaaagcag aggcgttgac aaatacacgc ggctgagtat tggcaaagaa cactcctgtc
    32401 agtatgaatt taaaacagac acttcgggaa tcgaggacta tccatgcatt gccaacttca
    32461 tccgccggat tttactggta tctgacaact ggagttataa tcgactcttc gaatttgttg
    32521 gccaaaaaga actgaacgaa gggttacgac gcaaaggata caccgatccc gttattctga
    32581 aacgctttgc cagatgtgat gacgacgaaa accgctacac caatcctttt tgtttttata
    32641 acgacgatgg caacattctg ttgcagcagc cacagcttga aaaccggact gaatacagat
    32701 tgccgccaca cgtcaatgcc agcgtaggca aaggtttctg ggatggaaag aaagttttgg
    32761 aacagccccg cgatttcagc aacaacaact atctgccgct cgaagatgcg ctcgaaatgc
    32821 tcatttcatt catcatgccc gaagcagttg atgagcagaa gcgctggaac ctgagtccgc
    32881 acgaccggct gtttttattg caatattttt cgatgtatcc tcgtgaatct ctttttgaag
    32941 aatatcacaa ctggcccaaa tacgaagaca gctacaaaaa atattttctg atgggcgacc
    33001 acaaagacac catttacgat ggggctctgc gcgttttcaa cgtagtgggt ctcagctacg
    33061 gctttgcatc ggatgtggcc tatgtggtca acctccgcga aggcgttgag ttttttctgg
    33121 ccgccaccat ttacaccaat cacgatgata ttctgaatga tgaaacctac gagtaccgca
    33181 acattgcatt cccgtttttc ggacgcttcg gaagaatcat ctatgacatg gagcgtgcac
    33241 gtgaaaagca gtatctgccc gattttagag agataaagca ggctttgtcg ttgccaccaa
    33301 tgtaaaggga aacggtgggg tgcataatct tcgtgaaaaa acctttttat caattctgaa
    33361 aaaagccata tatgtagggt ctgctgaaaa agtctgaggc gcgccagatt tgaggcgaca
    33421 agacg
//
LOCUS       DAOPKE010000024        32822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1422_length_32822_cov_11.054659, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000024 DAOPKE010000000
VERSION     DAOPKE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_32822_cov_11.054659"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..644)
                     /locus_tag="PKY63_04835"
     CDS             complement(303..644)
                     /locus_tag="PKY63_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99975.1"
                     /translation="MKTLSISGLVLALLGIAVGIYNQLTYVAAYHAHMCKTDMLSRAD
                     CETTQNMQVLLGQTSVILGALAFVMCVLPAFRNKKDLLAFFGIFFSIVSILIGLMQAT
                     HIFDYTGYFAR"
     gene            797..1093
                     /locus_tag="PKY63_04840"
     CDS             797..1093
                     /locus_tag="PKY63_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPE99976.1"
                     /translation="MKKRFTTVVKEKEKTIPDSESTTKLPKSLILINDDVNTFDHVIE
                     TLIEICNHNEEQAEQCALITHLKGKCPIRKGERNELLHIKQQLSDRMLSTVISE"
     gene            1108..1911
                     /locus_tag="PKY63_04845"
     CDS             1108..1911
                     /locus_tag="PKY63_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPE99977.1"
                     /translation="MIKRISLLIVLAILFADCSRVPVTRRKQFRMLPESMINSMSLTA
                     YNDFITSNQVVGTTDSRTQQVVNVGQKLQNATITYLKKHGYGKRVKQFSWAFTLVEDP
                     TVNAFCLPGGKIVVYSGILPVTKTDAGLATVLSHEIAHAVARHGNERMSQQLAVAMGG
                     VALSVAIQNNPQETQSIFNSVYGIGGNLGILAYSRKHEYEADKIGMVFMALAGYDPAE
                     SIVFWETMSASGAGANIPQFLSTHPSDANRIAAMKEFLPTAKTYFKPAQ"
     gene            1918..2382
                     /locus_tag="PKY63_04850"
     CDS             1918..2382
                     /locus_tag="PKY63_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPE99978.1"
                     /translation="MDWTLIITLISTGILLILLEIFLLPGMVAGILGGAATLFGIYQS
                     YAVYGPEAGIITTVVTLALTVVLLILLFRSKTWKKAALDNAIDGRMNTESDKLNPGDE
                     GKTITRLYPIGKAEINDNIYEVQSLSGIIEAESDIVVTKIEGYKIFVKPKNT"
     gene            2404..3399
                     /gene="floA"
                     /locus_tag="PKY63_04855"
     CDS             2404..3399
                     /gene="floA"
                     /locus_tag="PKY63_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244976.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPE99979.1"
                     /translation="MLKSNAEVGIYFAIGIGALIALWLLFYLIPVGLWFNALLSGVRV
                     SLVQLILMRWRKVPPAIIVNALITSFKAGIPLNRNELEAHFLAGGRVKNVINALISAD
                     KANIPLDFKSATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLIAKARVTVR
                     ANIRQLVGGAGEETVLARVGEGIVTSIGSSFSHKEVLENPDKISKVVLARGLDAGTAF
                     EILSIDIADIDVGKNIGAILQMDQAQADKNIAQAKAEERRAMAVAQEQEMIAKAQEAR
                     AKVIEAEAEVPKAMAEAFRSGNMGIMDYYKMKNIQADTDMRQNIAKPGPQGPEGK"
     gene            complement(3552..4901)
                     /gene="murD"
                     /locus_tag="PKY63_04860"
     CDS             complement(3552..4901)
                     /gene="murD"
                     /locus_tag="PKY63_04860"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPE99980.1"
                     /translation="MYQELNNLIDYLKGTKILIAGFGREGKSTLRFLLKHVPDVSVSV
                     GDENVFDFNDEFAGLDIPIMSGETFFGNPDDFDLMIKTPGISLKKLPESWLKSDKITS
                     QTDLLLKFFANKVIGITGTKGKSTTTNLVHHLLVSNGVKAILAGNMGIPFFDELENAD
                     HKMIVAELSCHQLENVYSSPVISVILNIFPEHLDHYESFEAYARTKWNVGLYQKWGGR
                     LFIPDDWLKKEWLVYQKQYSGQLTGFGAKDDEIIYKFDETCFEQKLDLDRILLQGSHN
                     LKNIAAALGVAVEAGVCLSDAIVSLYNFKPLPHRLEFIKEVNGVKYINDSISTIPQSS
                     IAAMKAYPKTETIILGGMNRGIDYSALIDYLLKSDVRNVVLMGEVGDIIGKEMEVRQS
                     RISLHYAGDMQQAVEFASLNTTAGGVCLLSPAAASYDKYINFEYRGDDFRKCVESLK"
     gene            complement(4882..6258)
                     /locus_tag="PKY63_04865"
     CDS             complement(4882..6258)
                     /locus_tag="PKY63_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99981.1"
                     /translation="METVDKNASFVEFREKYPVFTYESFDYKLTERGISFSFHFHIGD
                     EWLFRPTSEIDFQQVPLLKNIPETEIENLVFHIGMIELISYWKPTCSPSVVIKPYKLT
                     EEQIRWWKKLWYNGLGEFFYINSIQTSAKDFLWISSGEKELSKCRFDVENLLSTLVPI
                     GGGKDSPVTLELIKNNGRNVIPLIINPRGATLNTVKMSGLPMDAFVLVKRSIDQVLLE
                     MNTRGFLNGHTPFSAMLAFYTLLIARMTGVSDIALSNESSANESTVPGTDVNHQYSKS
                     FEFEQDFRDYYKKYISEEFNYFSFLRPLTELQIAGLFSRMTAYHAVFRSCNVGSKDDK
                     WCGHCPKCLFANIILSPFLSPQELIKIFGENLYEKMELKSAFDELTGHVPVKPFECVG
                     TVSEVNEAIQLAIQKYYTGAGTLPFLLSIYVKRHSELVVNSPGFWNKEHNLSSFYEKI
                     LHHVSGAE"
     gene            6327..6899
                     /locus_tag="PKY63_04870"
     CDS             6327..6899
                     /locus_tag="PKY63_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPE99982.1"
                     /translation="MFEIKSNAVAPATGRVLISEPLLQDFYFRRSAVLLVDHGNDGSF
                     GVIINKPIKTNISEIMPDLGDFDAPLYIGGPVDPKSIFFIHSHGDIIEGSEHIGDNLF
                     WGGRITSVVDNILAGNISPDTIRFFLGYSGWDANQLEEELKKNAWAVTVPKLNELIQM
                     KPELVWSGLVKELGDEYHFWTHLPADPQFN"
     gene            7154..8458
                     /locus_tag="PKY63_04875"
     CDS             7154..8458
                     /locus_tag="PKY63_04875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE99983.1"
                     /translation="MIASAIENLHIKELNSMQLDAIDSIEKNQNTILLAPTGSGKTIG
                     FLLPILKMLDTNKDQIQALVLAPSRELSIQIEKVFKSMKTGFRSLCCYGGHAVSVEKK
                     SLQARPTLLIGTPGRIADHLRREHIDTTGIQILVLDEFDKSLEFGFENEMSFIIGRLN
                     QLKKRVLTSATQSLIIPDFTGLNNPATLNYLSTGPAEGLSLKMVISDSIEKTETLFRL
                     LCHLGNHSSLVFCNHREAVERISRFLSERQLENDIFHGGLEQDKREHALIKFSNGSVR
                     VLITTDLASRGLDIPLVENIIHYQLPSTETAFTHRNGRTARMHAQGNAFLLLSGEEKL
                     PAYITEKPPVEPLPEKTVIPKPSGWTTLYISAGKKDKVNKVDIVGLLLQKGKLQKDEL
                     GLIHVLDFVSFIAVKSNKIPELLRLLRDEPLKKKKVKIAVAR"
     gene            complement(8653..11592)
                     /gene="dnaE"
                     /locus_tag="PKY63_04880"
     CDS             complement(8653..11592)
                     /gene="dnaE"
                     /locus_tag="PKY63_04880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPE99984.1"
                     /translation="MILHCRSYYSLRFGMMAPSTVATMAAACGFPKVLLADINNCTGW
                     VDFIKQCRSERTEGMVGMELRRGSRLLYVCMARNMSGFQEINEYLTECNMTESQPADF
                     APPFNQVFVLYPWNENTPKQLRANEYIAVRPSQVPQLRVLNDRKVRGHMVAWWPVTFE
                     SEPDYDTHCHLRAIDSNTLLSNLKSTQLAMNDEIMPDRKAAYEAFSECPDLLANAENL
                     LRDCAADVQMGIVRNKKTFTGSRYDDKLLLEKLAMDGFPARYPGKPKPVLERIRKELE
                     IIDRLGFSSYFLMAWDIVRYSMSRGFYHVGRGSGANSIVAYCLYITNVDPVELDLYFE
                     RFINPKRTSPPDFDIDFSWKDRTYVQDYIFKRYGSKHTALLGMMQTFRDKSVYRELGK
                     VYGLPKTEIDALVEERKSLKADDDIIRKIVSVGEAIRDFPNLRSIHAGGVLVSEDPVT
                     MYSALDLPPKGFPTVQWDMYVAEDLGYEKFDILSQRGIGHIGDAVKFIAQNRREKIDI
                     HDIPAFKRDPAIAAQIEKGETIGCFYVESPAMRSLLRKLDCRDYLTLVAASSIIRPGV
                     SSSGMMQEYIRRYHNPKSFHYLHPVLEELLHETYGVMVYQEDVLKVVHHYAGLDLAEA
                     DVLRRAMSGKYRSKEEMMRIVKGFHEGAAKMGRPEEVTKELWRQIESFSGYSFSKAHS
                     ASFAVESYQSLFLKAHYPVEFMTAVINNFGGYYRSWVYFSEARRSGANILLPCVNTGD
                     YITTVKGNDIRIGFIHVQGLEENFIRPVLEERLRNGPFAGMADFMARIHTTREQLVLL
                     IRTGAFAFTGKTKPELLWETLAGQDARKIPEASGQLFASSHRLPELPSLETSQLEDAY
                     DEIELLGFPVTYTWFELLKTGFRGEIQASGMASAEGKYVRMIAPLVTIKPVRTKKNEK
                     MYFASFIDFEGNFFETVHFPDSLRKYSFRGNGVYLLYGKVINDFGALNLQVEKMAKME
                     LRGDPRA"
     gene            11995..13260
                     /locus_tag="PKY63_04885"
     CDS             11995..13260
                     /locus_tag="PKY63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99985.1"
                     /translation="MNKEVLKQILFEQRKDFLKKGIGVSREKLTEAEKFLQLPHIYVI
                     SGIRRCGKSTFLRQIAHTFYNDKDFYFINFEDERLFNFNASDFNLVLETQNELWGSQK
                     TFIIDEIQNVDKFELFLRRLTDSGYKFIVSGSNADLLSGEMATKITGRYIETKLNPFN
                     FREFLNYHRISTDINDVFDTENKAKISANFEKYINSGGMPEYNKFKMAEIITRMYEDI
                     LIKDIAVRYGITNIVALRELSRYVVSNFGRRFSYNSLQKALGFGSVNTVKSYLSYLEN
                     AFLLKIVSKYDHSLKKQLSNEKKTYIIDSAIIKLVSNQLTEDRGRLLENIVAQHLAVD
                     NQIYYFSDKYECDFISMDNHKNIAAYQVTYILNETNREREIKGLVEAMDFFGLNKGVI
                     VTMSQSEEIKISKKQITVLPAWRVLLNYA"
     gene            complement(13600..14373)
                     /locus_tag="PKY63_04890"
     CDS             complement(13600..14373)
                     /locus_tag="PKY63_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPE99986.1"
                     /translation="MKKINSIIGFLVLHFSLVAQVSGIDSVLVNKVSIDSIQKRFLLG
                     KFNPSQEAGFSLISSKYTLKKGIYLRTEVYNAFIAMRDSALKDGITLTIVSATRTFEH
                     QKMIWENKWNGRQYVGGKLLNTAYPDETERAQVILKYSSMPGTSRHHWGTDIDFNSVD
                     PAYFKTAYGIKVLQWLETHAAHFGFCRPYTPFGETRKTGFQPEEWHWSYYPIAYKFQQ
                     AYKKQINYVDIEGFDGDETVFSVRVIENYVLGVDSVCWK"
     gene            complement(14370..15326)
                     /locus_tag="PKY63_04895"
     CDS             complement(14370..15326)
                     /locus_tag="PKY63_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPE99987.1"
                     /translation="MKSYFNRENRPTRFGFGEGVRDAARRNPSVVGLGADITSSVSMN
                     FFAGEFPDRFFSMGIAEQNCVAVASGLALTGLIPVFSTYAVFSTLRAADQIRVSVCYN
                     NLHVIIGGAHAGISVGPDGATHQALEDIAVMRVLPNMSVISPCDATQARLATICAVNE
                     LSGPVYIRFGREAVPDFTSEVDGFQFGKGQLMREGSDLTIVATGHMVWHALQAAEQMA
                     AQQISTRVINIHTIKPIDREIIIHAARETGAIVAAEEHQIAGGFGSAVAEVLAEEYPV
                     PVEFVGVNDCFGESGQPDELMQKYGLSAENIVAKSLKVLKRK"
     gene            15428..16882
                     /locus_tag="PKY63_04900"
     CDS             15428..16882
                     /locus_tag="PKY63_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPE99988.1"
                     /translation="MENVFQNLKPEKVWQYFAEICEIPHPSKKEEKMVKYLLNFGKHH
                     NLETVSDEVGNVVIRKPATPGYENKPWVALQSHIDMVCEKEAGSTHNFDTDPIIPRID
                     GEWVKATGTTLGADDGIGVAAQLALLDATDIEHGPIECLFTVDEETGLTGAFALKPEM
                     LKSRILLNLDSEDEGEIFIGCAGGKDTVGTRYYTTEAAPEGMSAVEITVGGLKGGHSG
                     DDIHRGLGNANKILGRALWEISNDHPAARLVSFDGGNLRNAIARDAVAVFLIPSGKEK
                     EVFTYIENLDKTVKNEFKITDPLVHVEIKKTNNPGQVIDSKTANKLIDLIYAMPHGVI
                     AWAQDIEGFVETSTNLASVKMSNNTITISTSQRSSVASAKQNICNAVAASFRLAGFDY
                     KHGDGYPGWTPDPNSAILKITVEQFEKQFGYKPVVRAIHAGLECGLFSEKIPGMDMVS
                     FGPTLRGVHSPDEKLLIPTVERFWNLILNILKNL"
     gene            16895..17335
                     /locus_tag="PKY63_04905"
     CDS             16895..17335
                     /locus_tag="PKY63_04905"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HPE99989.1"
                     /translation="MRKLILALALTGVMISQTGCFGEFGLTRKVYEFNQGVGGKFVNQ
                     IVFWAMCVIPVYEVAGMLDVLIFNTIEFWTGSNPVAMAPGETEVQYATVDGHQFRMTA
                     QQNYFVVEELTANGYEMRNEFRFNKDANELSISNGSETQVVAMN"
     gene            complement(17699..18298)
                     /locus_tag="PKY63_04910"
     CDS             complement(17699..18298)
                     /locus_tag="PKY63_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPE99990.1"
                     /translation="MALTKSRNRWKWVRWPRIILAKAILRLFGWKYNYAVSKDITKAV
                     MVVAPHTSNWDFILGRLCYNALDIRVRFMIKKESFVFPLNLIIRAWGGVPVDRKNPGN
                     IAEQMAAQFAKYDELVLGITPEGTRKPNPHWKKGFYRIAMQANVPMILTYLDYKKKFG
                     FVGKVVIPSGDYAADMKIMSEFFAECTACHPENYVTPQF"
     gene            18378..19991
                     /locus_tag="PKY63_04915"
     CDS             18378..19991
                     /locus_tag="PKY63_04915"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE99991.1"
                     /translation="MKQIFLIVALILSFQQYSFSQSDEYPLSESCTSIMAGKNATSDG
                     SVITSHTCDGRYRSWLNIIPGAKYEDKAMMDIQWGTMMTESPWDTRGVETKGKIKQAK
                     ETFAYLNVAYPCMNEKQLIIGESTISGRKELVNNKGLFLIENLEAIVLQRCSTARDAI
                     NLIGELVKEYGYADGGECITIADPKEVWVLEIFGEGPDKIGAVWAAQRIPDDHVGVCA
                     NISRISEIDLKNKDYFMASDNVFSVAKKMGFWDGKETFKFWKAYSGSKPFQIREYFIL
                     SSLAPSLNLDYNAEELPFSVKPDNKVSVRDVMALLRSTFEGTQWDMTQNLKMVSSYKN
                     EAGETVSDTITSPYANPWMKREMMGMLNYQNDTSVKFQRTCAVAWCAYSFVGQCRDWL
                     PDEVGGVCWFSFDNPAESPRIPIFAGTLSLPQSFNYSGQFAFNEQSAHWQYRQANRLA
                     TVRWGDGRKLIEPALMQYEDKAFAELADVEKRAAELLKEDQANAAAGQPTQKCREYLT
                     RYTADFHASSAAKWLELRNQLWTDLKLSF"
     gene            20236..21549
                     /locus_tag="PKY63_04920"
     CDS             20236..21549
                     /locus_tag="PKY63_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE99992.1"
                     /translation="MIDQKELLQSLILDFQKKLPLKIFSRDEELPTDSKKVISVCGVR
                     RSGKTFVLFDTINRLIKSGVPKTEILFLSFDDERLQLKRQELDLILQSYRELYPKTDM
                     QNVYIFFDEVQMADGWEQFVRRIYDSVSKHIFISGSNSKLLATDIATSLRGRSLQFEV
                     FPMSFAEYCRFRNIDTNTRDTETKAQVYSGFNEYMRFGGFPELVMNQYRHLPQTIQEY
                     YHVMLYRDLIERYQVKNIPVLKYFISRLLANHTSPTSINKIYNEIKSAGLKTDKNVLY
                     DFSEQLEAINFTQRLRKFDNSVLKTELASERKVYFIDNGFLQALKQTTSKDNDKLLEN
                     AVFMHYRRQTPFMRGLYFYKKNKECDFVVLDREKPFEIIQSCYDFSSPDTRKREISAL
                     LEASDYLKCNNLKIVTLDPNEEIKAGSKTIRVINAAEFMLGHKQK"
     gene            21691..22128
                     /locus_tag="PKY63_04925"
     CDS             21691..22128
                     /locus_tag="PKY63_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99993.1"
                     /translation="MNSVTFHPMVVHFSVALVIVGFVIDLFYIFIAKKDWRKTSSLFL
                     MILAVLAVFFSWFTGEYYSRSYDGVGRDVKDEHELYAWITMGVFGAATAVRFIAIYLK
                     KNPAFFKYLYIVLMLSAVMAIGYCGYLGGNLVYELQHDLRPGQ"
     gene            22154..24562
                     /locus_tag="PKY63_04930"
     CDS             22154..24562
                     /locus_tag="PKY63_04930"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPE99994.1"
                     /translation="MYSILFRLLFIASLLISSINTVWSQDITFQIRNKSREPLIGSTI
                     KLSAEKDSTKMFLAAADVNGAAVLKNIPDGMYHVVVTYIGYTTFEKTIAVNPQHRHFS
                     FSLTENSLALNEVTITADRPFIRQEDDKMIVDPTPMIGISTNTLEVIEATPGIYVDYD
                     AGIFLSSTTPAVIYINGREQKLSNQDLMNLLRNLPPGSIERIEILRTPSTKYDAASSG
                     GIVNVVLKKGLKIGRFGNINVGMNQGKYGNRIAGANFNNSGDKTTLYLNVGFNRHDRL
                     DEMQTVRLTATDTTLNQQSETRQTSDDLFLGYGLTWDSDSLSGFSYDGRFSFGNRNSD
                     VFNHSFFADETDQHLLESQDQINTTIDRLSLSQDIGYSYKIDTLDSECDTRLGFDISR
                     NTSAQHFISEYTFPYDTMISGLMNNHQNRIIIQLASDLTLQLKNGIKTESGLKGSWQQ
                     YNSNADFFTNSNAIQVADSSRNNNYTFNESIFAAYTQASKTVWGGLVLKAGCRLEYTY
                     MNGQQKLPYDTSFLVNRADLFPYLYISRDLPTLLPNIIPIKLRTFLIYRRTIARPEYG
                     QLNPSHEYVDPYLYEAGNPDLKPQFTNNIEFNISYQDMPILAFGINYTQDVFSAVVYE
                     DPNQNGIARMTYDNLGKNTEKYMRGMIGVPPGGRYFFALGTQYNLNNYDGFYEGEPLK
                     YERGSWRFFTFHSLKLTQNTKFNISGFMMVKGSRGFYELGTFGSLNAGLSQTFMNQKL
                     IVTINARDILGTMNISFNLNQGSIPSYGERYRDSQRVGINIRFNFGLPKREKTNGFEG
                     FEED"
     gene            24745..25374
                     /locus_tag="PKY63_04935"
     CDS             24745..25374
                     /locus_tag="PKY63_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPE99995.1"
                     /translation="MKASEKLIITISRQLGAGGAYIGQQIARKLKIHYADHEIIRKAA
                     EKLSVKEEDIERRDEKTQPFWETMLFSSGYAPEFYVPINIKYAPTDAEMYNVEMSIMQ
                     KLAENIDAVIIGRGGFHIFKDRPNTLKVFLFAPKEYRIDRVMTEHNLNEAEAKKAIED
                     SDRERAKYIDAFTGLKWIESTNFDLTLNTSKLGLDKTAEAILAMINLMK"
     gene            25483..26115
                     /locus_tag="PKY63_04940"
     CDS             25483..26115
                     /locus_tag="PKY63_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPE99996.1"
                     /translation="MKTSDPLIITISRQMGSGGSYIGQKLAKKLKIHYADREIIRKAA
                     ETLSVPEEDLESMDEKSQTWWDYIQLSSRYASEIYIPPVQKFAPTDLELFKAECEIIK
                     QIAKETSAVIIGRCGFCVLDGHPNLIKIFLHGEKDFRVKRIMDLYSVSIKTAEKMVED
                     SDRKRGLYIKKFSKKDWMNIDNYHLSVDTSKIGIDSSLDLISAFVANARK"
     gene            26247..26894
                     /locus_tag="PKY63_04945"
     CDS             26247..26894
                     /locus_tag="PKY63_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99997.1"
                     /translation="MKYLSVLLLACSIASCGNKDKQTEVVRELNPTDTLDKLMLIKNE
                     AELIEKFGQENVTWDTVYGAEGAVSMATLLYKGTCDQVEITWENLKNKSAIQKVTLNA
                     LYDEKQEKLIAFSHWKTSYGIMLGTSLKELETINEAPFIFLGFGWDYGGIVTELNGGK
                     LSNLPVSIQLGISDIAKQSNNPDYEMLMGDNEFNSIDAEAQRLNPVVVSISVLKK"
     gene            26909..27244
                     /locus_tag="PKY63_04950"
     CDS             26909..27244
                     /locus_tag="PKY63_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99998.1"
                     /translation="MKINKTVAGVIAGFTAGIIDLIPMIIQKLTWDANLAAFSMWLAV
                     GFFTAHTSFRMQPALKGILIAFICLLPSAFIIGWSMPESLIGVAAMTLILGALTGLLV
                     HWMTKEKSA"
     gene            complement(27267..27773)
                     /locus_tag="PKY63_04955"
     CDS             complement(27267..27773)
                     /locus_tag="PKY63_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE99999.1"
                     /translation="MKKLLLIVFFATVFSSADAQYPLEAGRLQFNFGTGYTNQGIPAY
                     LGADFGIAENLSMGLDGSYRFFNENFGNVMYKHSIITVLGNVNYHFNTMFKLRDHTST
                     DLYGGFSGGYVMAESPALYNGGVETSAVFGAQFGYRMFFNEKFGLDLQLNAMSTYMGA
                     RIGLTILL"
     gene            27933..28505
                     /locus_tag="PKY63_04960"
     CDS             27933..28505
                     /locus_tag="PKY63_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPF00001.1"
                     /translation="MSIKGTKTEQNLLKAFAGESQAARRYKMFAKIAIKEGYQQIAAI
                     FEETASQEDQHSKRFFEFLEGGMVEITAAYPAGVLGTTAENLEAAASGENEEWTELYP
                     EFAKIAEEEGFKKIATAFKMIATVEAQHEKRYRKLLKNVIDGKVFEEEEEVEWICRKC
                     GYVHKGKKALKNCPACEHPIDYQERKASNY"
     gene            28510..29175
                     /locus_tag="PKY63_04965"
     CDS             28510..29175
                     /locus_tag="PKY63_04965"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPF00002.1"
                     /translation="MNFAFSGKRMKYFLFLFFIVTTIAFCFGSPYPELMMLHHSATIL
                     TAAFLVYIILKNNLSNLSFLFIILFILLHVIGAKWTYLDVPYDQWWNWLSGDSLSSVF
                     GWERNQYDRLVHFGFGLLIWLPAYEILQRWFGYKKTHARYVAWEFIAMASVVYELFEW
                     LLTLVAPANQADNYNGQQGDMWDAQKDMALALLGASMAWLAFLIKKWYSSRNQSLISK
                     RYL"
     gene            29393..29749
                     /gene="rplS"
                     /locus_tag="PKY63_04970"
     CDS             29393..29749
                     /gene="rplS"
                     /locus_tag="PKY63_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPF00003.1"
                     /translation="MNKGELIKFVENQLIDSKQFPMFKSGDTVTIKYKITEGNKERIQ
                     AFQGIVLQISGKGANKTFTVRKISNNIGVERIFPFSSPFIDGIDVNKHGVVRRSRIFY
                     LRELRGKKARIKEKKY"
     gene            complement(29998..32007)
                     /locus_tag="PKY63_04975"
     CDS             complement(29998..32007)
                     /locus_tag="PKY63_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPF00004.1"
                     /translation="MKHLFRSCMVVFLFFLVQPTNVSAQNRDMVSLDGEWNFVVDPDA
                     RGLQLGWGEKGLPPRGKRLAIVPHTFNTNEGYYLYWGAGWYDRKFQITQNQMKKTVQM
                     RFEAVNHDAIIWINGKKAGEHFGSGYTPFSIDITPYIQPGENIVTVYTNNEPSKKSIP
                     YLKSFDWSHDGGIIRSVNLIFTEEQAMRTIHVNGIPEGNGGRAEISIPLIDFSKINGS
                     KTYFIAVITEENQKTRNVVFKGKLKGKFSHGRFLSTLNLKKVNRWHFDSPDLYRIDIT
                     MFEGKTAKDELHAVFGFRSITIENNRYVLNGEPMRLMGMEWTVGEDMNHGLAQTDENL
                     IKGLTLMKNANCVFARSHWQQSEKFFDWCDRNGILVMEEVPLWGWEPHLNDSLLPVAI
                     QHLDEMIEAHYNHPSIIIWGLGNELSSHEEVNINGLDAMEKHARSLDHSRLVAYISNA
                     VHWEVPGPKNILPDASPRYDMIMYNEYNTTWYGYNLDSIPLALDRIHQLYPEKPITIS
                     EWGICEPKFKGGDERRAMEMAEQIKIYGSKEYVAGAIYFCLNDYRTHFGEDSTYHYPQ
                     RIHGICDVKLNPKPSYDVMKEISSPIILKDLIVNGNDVSLTLVGNTGIPSYTVRDYYL
                     LAGETRIEIIQLKPGQEIKVDFSTSADDAVIYRPTGFEVMRIGLK"
     gene            complement(32133..>32822)
                     /locus_tag="PKY63_04980"
     CDS             complement(32133..>32822)
                     /locus_tag="PKY63_04980"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HPF00005.1"
                     /translation="EVDALCKKYNVDAIVSLETFDSDIRRVSRSRQVERAVNGENVMV
                     TEFIEDLNIRVNAGWRIYDNVNKKIIDQDVFYDDKGWSTVGPTPEDAFRKLPSKRNAI
                     NDAGYFAGEMMGIRISPNWVRVSRIYYKKGDDRLKSAKRFVKVNNWKEAVVLWSQSAK
                     SPDPKIAGRACHNMALAAEMEGELSIALEWANKAYKDFGLRKERAYINELNLRLMEVE
                     KLKEQLPESGN"
BASE COUNT         9782 a         7257 c         6829 g         8954 t
ORIGIN      
        1 tttgctgaat atatttctgt ttttgtgagt gttacgtgtt tttcagcaga ccctaagtaa
       61 actgattgta taaacctgct gcaaaaccga gcaaggaaat aataaaaccg gaaagagaaa
      121 gagttttcat gatttcagga atttatgaca aatgattctg caaagctaaa tatttttaac
      181 tatacaaaac aacttttagt aatttaattt acatcgttga tatgtaaata atgaccaaaa
      241 tcaaatacta taagtctgaa ttatcatggt cagacctatt gtttttttta accagcaact
      301 gcttatctgg caaaataacc ggtatagtca aaaatatgtg tggcttgcat caaaccaatt
      361 aaaatggaaa cgatggaaaa gaagatgcca aaaaaagcca acagatcctt tttatttctg
      421 aaagccggta aaacacacat aacaaaggcc agtgcgccaa gaataacaga tgtctgaccc
      481 aataaaacct gcatattctg agttgtctcg caatcggcac ggctcagcat atcggtcttg
      541 cacatgtggg catgataagc cgccacatag gtaagctgat tgtaaattcc aacagcgata
      601 cccaaaaggg caagcactaa tccagagata gataaagttt tcattgcagt agggattaaa
      661 atcatttata tgtcgctgaa tacaaatata atgaatttaa cataagcatt ttttcacaca
      721 aacagcaaat cactctaatg ttaaaatgcc ggagatttaa aaaaatgatg gatttagatg
      781 attatttttt ttaattttga aaaaaagatt tacaactgtg gtaaaggaaa aagaaaaaac
      841 aatacccgac tcggaaagca caacaaagtt gcccaaaagt ctgattctta tcaatgacga
      901 tgtgaatact tttgatcacg ttattgaaac gctcattgag atttgcaatc acaacgaaga
      961 acaggcagaa caatgcgcac tgattacaca tttaaaagga aaatgtccaa tcagaaaagg
     1021 cgagcgtaat gaattgcttc atatcaaaca acagctttca gaccgcatgc tttcaacagt
     1081 tatcagtgaa taataatcca tttttttatg attaaacgta tttccttgct tattgtgctg
     1141 gcaattcttt ttgccgactg ttcccgtgtg cccgtaacgc ggcgtaagca gttcaggatg
     1201 ctgcccgaaa gcatgatcaa cagcatgagt cttacagcat acaatgactt tattacttcc
     1261 aatcaggttg taggcaccac cgattcacgc acacagcagg ttgtcaatgt tggtcagaaa
     1321 ctacaaaatg ccacaatcac gtacctcaaa aaacatggtt atggcaaacg ggtcaaacaa
     1381 ttttcctggg ctttcacact ggttgaagac cctaccgtta atgccttttg tctgcccgga
     1441 gggaaaatag tggtgtattc gggtattctt ccagtcacca aaaccgatgc aggtttggcc
     1501 actgttctga gtcacgaaat tgcccatgcc gttgcccgtc atggaaacga acgcatgagc
     1561 cagcaactgg cagtagccat gggaggtgta gctctttcgg tagcgattca aaacaatcct
     1621 caggaaacac agagtatttt caattcggta tatggaatcg gtggaaatct tggaattctt
     1681 gcctactccc gaaaacatga atacgaagcc gacaaaatcg gaatggtttt catggctctg
     1741 gcgggttatg atccagccga atccattgtt ttctgggaaa ccatgtcggc aagcggtgca
     1801 ggtgctaata ttcctcaatt tctcagcacc catcccagcg atgcaaacag aattgctgcc
     1861 atgaaagaat ttctgccaac tgcaaaaact tattttaagc ccgctcaata aaaaactatg
     1921 gactggaccc tcatcatcac tttgatttca acaggaatcc tgctgatttt actggaaatt
     1981 tttctgcttc cgggaatggt tgcaggtatt cttggcggag ctgccacttt gtttggtatt
     2041 tatcaatcat acgctgtata tggtcccgaa gcaggaataa tcactaccgt tgttaccctg
     2101 gctctaaccg tagttcttct cattttatta ttcaggtcga aaacgtggaa aaaagccgcc
     2161 ctcgataatg ccatagatgg cagaatgaat accgaaagtg ataaactcaa tcccggcgac
     2221 gagggcaaaa caatcacccg gctctatcct atcggtaaag ctgaaatcaa tgacaatatt
     2281 tacgaagttc aaagtctgag tggaatcatc gaagctgaat ccgatattgt tgttacaaaa
     2341 attgaaggtt ataaaatctt tgtcaaaccc aaaaacacat aaaccatgaa tttacttttc
     2401 gtcatgctta aatccaatgc tgaagtaggc atttattttg ccatcggtat tggagctctc
     2461 attgccttat ggcttctgtt ctatctgatt cctgttggac tgtggttcaa tgccttactg
     2521 tcaggtgtcc gcgtttcgct tgttcagctc atccttatgc gttggcgtaa agttccgccc
     2581 gcaatcatag tcaatgcgct gattacttct ttcaaagcag gaattccact caatcgcaac
     2641 gaactcgaag cccatttcct tgccggtgga cgggtaaaaa atgttattaa cgcactgatt
     2701 tcggccgata aagccaatat accgcttgat ttcaaatcgg ctaccgccat cgatctggct
     2761 ggccgcgatg tattcgaagc cgttcagatg agtgttaatc caaaagtgat taacacgcct
     2821 ccggttactg ctgtagctaa agatggtatt cagctgattg ccaaagcccg tgtaacggtc
     2881 cgtgccaaca ttcgccaact ggtgggtggt gccggcgaag aaactgttct ggcccgcgtt
     2941 ggtgaaggta ttgtaacatc aatcggatct tcttttagcc acaaagaagt gctcgaaaat
     3001 cctgacaaaa tttctaaagt agtgcttgcc cgtggccttg atgcaggaac cgctttcgaa
     3061 attctatcca ttgatattgc cgacatcgac gttggtaaaa acattggtgc cattcttcaa
     3121 atggatcagg cgcaggccga taaaaatatt gctcaggcaa aagccgaaga acgtcgcgca
     3181 atggctgtag ctcaggaaca agaaatgatt gccaaagctc aggaagcccg cgccaaggtc
     3241 attgaagccg aagccgaagt tcccaaagcc atggctgaag ctttccgaag tggcaatatg
     3301 ggcattatgg attattacaa aatgaaaaat atccaggccg ataccgacat gcggcaaaac
     3361 attgccaaac ctggccctca gggtcctgaa ggaaaataat tccggataat ttcaacgcaa
     3421 actccctggt caccagacct gggagttttt tttttattaa tttgaaatat aacagtccgg
     3481 agaaattgct tatgtttcat atataagcaa atacccatag aattgtttgg actgcaatta
     3541 gctcctcatc gttatttcaa tgattcaacg cattttctga aatcgtcgcc acgatattca
     3601 aaatttatgt atttatcata acttgctgct gccggagaga gcaaacaaac acctccagcc
     3661 gtagtattca acgatgcaaa ttcaacggcc tgttgcatat ctcctgcgta atgaagcgaa
     3721 atacggcttt gccgtacttc catttctttt ccgataatat cgcctacttc gcccatgaga
     3781 acgacgtttc gaacatcact tttcaacaga taatcaataa gtgccgaata atcaatgcca
     3841 cggttcattc cgccgagaat aattgtttca gttttcggat atgctttcat agcagcaatt
     3901 gaagactgag gaatggtcga aatagaatca ttgatgtatt tcacaccatt gacttcttta
     3961 ataaattcaa gccggtgtgg caaaggtttg aaattgtaga gtgacacaat ggcatcgctg
     4021 agacaaaccc cggcttcaac tgctacaccc agtgcagcgg caatgttttt caaattgtgt
     4081 gatccctgca acaatatcct gtccaaatca agcttttgtt caaaacaggt ctcatcaaat
     4141 ttatatatta tttcatcgtc tttggctcca aaacctgtca gttgtcctga atactgcttc
     4201 tgataaacaa gccactcttt tttcagccaa tcatcgggta taaacaagcg tccgccccac
     4261 ttctgataca aaccgacatt ccatttcgta cgagcatagg cctcaaatga ttcataatga
     4321 tcgagatgct ctggaaaaat attcagtata accgaaataa ccggcgatga atacacgttt
     4381 tcaagctgat gacaggacaa ttcagccaca atcattttat gatcagcatt ttcaagttca
     4441 tcaaaaaacg gaatgcccat attgcccgca agtatggcct ttacaccatt gctcactaat
     4501 agatgatgta caagatttgt tgtcgtgctt ttacctttgg ttcctgtaat tccaatcacc
     4561 ttgttcgcaa aaaacttcag caataaatcc gtctgcgaag ttattttatc tgatttcaac
     4621 catgattcgg gcagtttttt caaactgatg ccaggcgttt taatcatcag atcaaagtca
     4681 tcaggattgc cgaaaaaagt ttcaccactc attatgggaa tatccaatcc tgcaaattca
     4741 tcattgaaat caaatacatt ttcatcccca acactgaccg aaacgtcggg aacatgcttc
     4801 aataaaaaac gcagagtact ctttccttcg cgcccaaagc cagctatcag gattttcgtt
     4861 cctttaagat agtcaattag attattcagc tcctgataca tgatgcaata ttttttcgta
     4921 aaatgacgac aggttatgtt ctttattcca gaaacctgga ctattcacaa ccagctctga
     4981 atgccgttta acatatattg acaataaaaa cggcaatgtg ccggctcctg tataatactt
     5041 ctgaatggca agctgaatgg cctcattgac ttctgatacc gtgccaacac attcgaaagg
     5101 ttttacaggg acatggcctg taagctcatc aaatgcagac ttcagctcca ttttttcata
     5161 caggttttca ccgaaaattt ttatcagttc ttgcggggat aaaaacggcg ataaaataat
     5221 gtttgcaaat aaacatttcg gacaatggcc gcaccattta tcatctttgc tcccaacatt
     5281 gcaactgcga aaaacagcat ggtaagccgt cattcgtgaa aacaatccgg caatctgcaa
     5341 ttcagtcaag ggtctcaaaa atgaaaaata attgaattct tccgaaatat attttttgta
     5401 ataatctctg aaatcctgct caaattcaaa cgatttggaa tattgatgat tcacatcagt
     5461 accaggcacg gtgctttcgt tggcgcttga ttcattggat aatgcaatat ctgaaactcc
     5521 ggtcatccgc gctatcagaa gcgtgtagaa tgccagcata gcactgaaag gggtatgtcc
     5581 gttcagaaat ccccgggtat tcatttccag caatacctga tcaatgctgc gttttaccaa
     5641 gacaaatgcg tccatgggca gacctgacat ttttaccgta ttcagagtgg ctccccgagg
     5701 attgatgatg agtggaataa cattccggcc attgttttta atcaattcca gcgtcaccgg
     5761 cgaatccttg ccaccaccaa tgggaaccag cgttgataaa agattttcaa catcgaatct
     5821 acatttggaa agttcttttt ctccggacga aatccataaa aaatccttcg ccgaagtctg
     5881 aattgaattg atgtagaaaa attcaccgag accgttatac caaagttttt tccaccacct
     5941 gatttgttct tcagtcaatt tgtacggttt aattaccacc gatggagaac aggttggctt
     6001 ccagtaactg atcagctcaa tcatgccgat atgaaaaacc agattttcaa tttcagtttc
     6061 cggaatattt ttcaaaagcg gaacctgctg aaaatcaatc tccgaagtcg gacgaaaaag
     6121 ccattcgtca ccaatatgaa aatgaaaact gaatgaaatg ccccgctctg tcagtttgta
     6181 atcaaatgac tcataggtaa atacagggta tttttccctg aattcaacaa aactggcatt
     6241 tttatcaaca gtttccattt tggaacaact tttgagagga tttggtgtca aaagtaataa
     6301 agaatcacaa gtaaacaaca caagacatgt ttgaaattaa atccaatgca gttgcgccgg
     6361 ccaccgggcg ggttctgatc tcagagccgc tgctgcagga tttttatttc cgccgctcgg
     6421 ccgttttgtt ggttgatcat ggcaacgatg gttcttttgg tgtgattatc aacaaaccta
     6481 ttaaaaccaa tatcagcgaa attatgccgg atctcggcga tttcgatgct cccttataca
     6541 ttggtggacc cgttgatcct aaaagtattt tctttattca ttcgcacggc gacattatag
     6601 aaggcagcga gcatatcggc gataatttat tttggggtgg ccgcatcacc agcgtggttg
     6661 acaatatcct ggccgggaac atttcgcccg ataccattcg tttttttctt ggctattccg
     6721 gctgggatgc caatcaactc gaagaagaat taaagaaaaa tgcatgggct gtcaccgtac
     6781 cgaaactgaa tgaacttatt cagatgaaac cggaacttgt atggtcggga ctagtgaaag
     6841 aactcggcga tgaatatcat ttctggacac atttgccggc ggatccgcag tttaattagg
     6901 cgttagtcat caggggttag gcgtaagtca taaggcataa ggcgctagtc gtaaggtccc
     6961 gccttcgtcg ggataaacta caggcgttag gattgagacg ccaggtttca gcctaataat
     7021 tatttgcgac agttttcttc tgatttttca tttcttaagt gatgttttat tttatttaag
     7081 tcgacagctt atttagctgc gattcctcca tttaatttcg tagtatcttt gcagaatgac
     7141 gcacacgacc aacatgattg catcagcaat tgaaaacctg catattaaag agttaaattc
     7201 tatgcagctt gatgccatcg attccattga aaaaaatcaa aacaccatac tgctggctcc
     7261 aaccggttcc ggaaaaacca ttggattttt gctgcccatt ctcaaaatgc tcgataccaa
     7321 taaagaccag attcaggcac ttgtattggc gccttcgcgc gagctttcga ttcagattga
     7381 aaaggttttt aaatccatga aaaccggctt caggtctttg tgctgttatg gtggccatgc
     7441 tgtttctgtc gagaaaaaaa gcctgcaagc caggcccact ctacttattg gtactcctgg
     7501 ccgtattgca gaccacctca ggcgcgaaca catcgacacc acaggaatcc aaatactggt
     7561 gctggatgaa tttgataaaa gtctggaatt tggctttgaa aatgagatga gttttatcat
     7621 tggccggttg aatcaattga aaaaacgcgt acttacttcg gcgacacaat cgttgattat
     7681 tccggatttt accggcttaa acaacccggc aacgctgaat tatctttcaa ccggaccagc
     7741 tgaaggtctg tcacttaaaa tggttatatc cgatagtatt gaaaaaacag aaacattatt
     7801 tcgtttactt tgtcatctgg gaaatcattc ctcgcttgtg ttttgcaatc accgcgaagc
     7861 agtggagaga atcagccggt ttttgtcgga gcggcagctg gaaaatgaca tctttcacgg
     7921 cggtctggaa caagataagc gcgagcatgc actgattaag tttagcaatg gcagtgtccg
     7981 tgtgcttatt acgaccgatc ttgcttcgcg aggacttgat attccactgg ttgaaaatat
     8041 aattcactac caattgccat caaccgagac cgcttttacc catcgcaacg gacgtacggc
     8101 cagaatgcac gcacagggaa atgcgtttct gttgctttcg ggcgaggaaa aactcccggc
     8161 atatatcact gaaaagccgc cagtagagcc attgcccgaa aaaacagtca ttccaaaacc
     8221 ctctggttgg acaacattat atatcagcgc cggcaaaaaa gacaaagtaa acaaagtgga
     8281 tattgtcgga cttttgttgc aaaaaggcaa actacaaaaa gacgaattgg ggttgattca
     8341 tgtgctcgac tttgtttcgt ttatcgctgt aaaaagcaat aaaatcccgg aattacttcg
     8401 cctgctccgc gatgagccgc tgaaaaagaa aaaagtgaaa attgcggtgg cgagatagct
     8461 gcagaaggtt gtgtttcccc cgacattcta ccgtcagcaa gaacaggtct tcattgcagc
     8521 tttgctgacg tggaatttta tctgactggc aaggcagacg tcgggaagtc agagtctgta
     8581 gcacccaacg attccgggac cgctcctttt cacaaatcaa tgtctgtcgg acgccagaat
     8641 gttgggtaca cgtcacgccc gcgggtcgcc acgcagctcc atcttggcca ttttttccac
     8701 ctgcagattc aaagcgccaa aatcgttgat gaccttgcca tacagcaaat acacaccgtt
     8761 tccgcggaaa gaatattttc gcagactatc aggaaaatgc accgtctcga aaaaattccc
     8821 ctcaaaatca atgaagctgg cgaaatacat tttttcattt ttctttgtgc gcaccggctt
     8881 aatggtgacc aacggcgcaa tcatacgcac atattttcct tctgccgacg ccatgcccga
     8941 cgcctgaatc tcgccccgga agccggtttt cagcagttca aaccaagtgt acgtcaccgg
     9001 aaagcccagc agttcaattt catcgtaggc atcttcgagc tgcgacgttt cgagcgacgg
     9061 cagttccggc agccggtgcg acgaagcaaa caactgcccc gatgcttcgg ggattttacg
     9121 cgcatcctgc ccggccagcg tttcccataa taattcgggt tttgttttac ctgtaaaagc
     9181 aaatgcacct gtgcggatca gcagtacgag ctgctcgcgc gtggtatgta tgcgcgccat
     9241 gaaatcggcc atacctgcga aaggcccgtt gcggagacgt tcttcgagca ccggacgaat
     9301 gaaattttct tcaagaccct gcacatgaat gaagccgata cgaatgtcat ttcctttcac
     9361 cgtagtgatg taatcaccag tattgacgca gggaagcaga atattggccc cgctgcggcg
     9421 cgcttcggaa aaatacaccc agctgcggta ataaccgccg aaattgttga tgacagccgt
     9481 catgaactct accggataat gcgctttcag aaaaagactc tgatagcttt cgacggcaaa
     9541 cgaggccgaa tgcgcctttg aaaacgaata tccggaaaag gattcaatct gccgccacaa
     9601 ttctttggtg acttcttcgg gacggcccat tttcgcagcg ccttcgtgaa agccttttac
     9661 aatgcgcatc atttcttcct tactgcggta cttgccgctc atggcgcggc gcagcacatc
     9721 ggcttcggcc agatcgagtc cggcataatg atgcaccact ttcaacacat cttcctgata
     9781 aaccatgacg ccataggttt cgtgcagcaa ttcttcgagc accggatgca ggtagtgaaa
     9841 cgacttcgga ttgtgataac ggcggatgta ttcctgcatc atacccgacg acgacacgcc
     9901 cggccggatg atggaactgg ccgccaccag cgttaggtaa tcgcggcaat cgagcttccg
     9961 gagcaggctg cgcatggcag gactttcgac ataaaaacaa ccgatggtct cccctttttc
    10021 gatctgcgcc gcaatagccg gatcgcgctt gaatgcaggg atatcgtgaa tgtcgatttt
    10081 ttcgcggcgg ttctgcgcga tgaatttcac cgcatcgccg atatgtccga tgccgcgctg
    10141 gctcaagatg tcgaacttct cgtagcccag atcttcggcc acatacatgt cccactgcac
    10201 cgtcggaaaa cccttgggcg gcagatcgag cgcggaatac atcgttaccg gatcttcgga
    10261 caccagaacg ccgcccgcat gaatgctgcg cagattcgga aagtcgcgaa tggcttcgcc
    10321 cacagacaca attttccgaa taatatcgtc atcggctttg agcgacttcc gctcttcgac
    10381 aagcgcatca atctcggttt tcggaagtcc gtacaccttg ccgagctcac gatagactga
    10441 cttgtcgcgg aaagtctgca tcatgcccag cagcgccgtg tgcttcgatc cgtagcgttt
    10501 gaaaatataa tcctgcacat aagtccggtc cttccacgaa aaatcaatat caaaatccgg
    10561 cgggctggtg cgcttcgggt tgatgaagcg ttcgaaataa agatcgagtt cgaccggatc
    10621 gacattggta atgtagaggc aatacgccac aatgctgttg gcgccgcttc cccgccccac
    10681 gtgataaaat ccacgcgaca tggaatagcg cacaatgtcc cacgccatga gaaaatacga
    10741 agaaaatccg agccggtcaa tgatttcaag ttcctttcgg atgcgctcca gcactggctt
    10801 tggcttgccc ggatagcgtg ccggaaaacc gtccatggcc agtttttcga gcaataattt
    10861 gtcgtcgtag cgacttccgg tgaatgtttt tttgttgcga acaatgccca tttgcacatc
    10921 ggcggcgcag tcgcgcagca gattttcggc attggcaagc aagtcgggac attcagaaaa
    10981 tgcttcgtaa gcggctttcc ggtccggcat gatttcatcg ttcatggcca gctgcgtaga
    11041 tttcaggttc gaaagcaagg tgttgctgtc gatagcccgc agatggcaat gcgtgtcgta
    11101 gtcgggttca gattcaaacg tgaccggcca ccaggccacc atgtgtccgc gcactttgcg
    11161 gtcgttgagc acacgcagct gcggcacctg cgacggccgc acggcaatgt attcattggc
    11221 cctgagctgc tttggcgtat tttcgttcca cgggtacaac acaaaaacct gattgaacgg
    11281 cggcgcaaaa tcggccggct gggattcggt catattgcac tccgtcaggt attcgttgat
    11341 ctcctgaaag ccgctcatgt tgcgggccat gcacacatac agcaagcggc tgccgcgccg
    11401 gagctccatg cctaccatgc cttcggtacg ctcgctgcga cattgtttaa tgaaatcgac
    11461 ccagcccgtg cagttattga tatcggccag cagcaccttc ggaaatccgc atgccgccgc
    11521 cattgtggcc acggttgagg gcgccatcat gccaaatcgc agactgtaat atgagcggca
    11581 atggaggatc attgactgaa tgactatttt ttaatcactt aataggacta caatataatc
    11641 attttttatt ttatacacca actctcgaag ggttttgaac ccttcgagag tttagtttat
    11701 agtcgacact ttatgcattt caactcattt tgtcgattat agtcgacaga tattacagtt
    11761 tcgaaatagt ttatcgctga attaacatca aggatttttc actctcaaaa tcgctgtgat
    11821 aatcacttcc agcaaaccga atccaccccc agcacatagt tttcgatgac gtgcaaagga
    11881 gaaaagaata agttgtatgc aataattgtg cattgtgagt gaacaataat taacgttttt
    11941 atgcattata aatgaacaat atttcgtata tttgtgcatt ctaaatgaac aattatgaat
    12001 aaagaggttc tcaaacagat acttttcgag caacgaaaag actttctgaa gaaaggcata
    12061 ggagtaagca gggaaaagct cacagaagca gagaaattcc tacagttgcc tcatatttat
    12121 gtaatcagtg gcatccgtcg ttgcggaaaa tcaactttct tgcggcagat tgcacatacg
    12181 ttttataacg acaaggattt ttattttatc aattttgaag acgaacggtt gtttaatttc
    12241 aatgcatccg atttcaatct ggttctcgaa acgcagaatg aactatgggg aagccagaaa
    12301 acatttatca tagatgaaat tcagaatgtt gataaatttg aattgttttt gcggcggttg
    12361 actgattccg gatacaaatt cattgtttca gggtccaatg ctgatctttt aagtggcgaa
    12421 atggccacga aaattaccgg acgctatatc gaaacaaagc ttaatccttt taatttccgg
    12481 gaatttttga attatcatcg catttcaaca gacataaatg atgtatttga tacagaaaac
    12541 aaagcaaaaa tatctgcaaa ttttgaaaaa tacatcaaca gcggaggaat gccggaatac
    12601 aataaattca aaatggcaga aattattacc cggatgtatg aagacattct gattaaagat
    12661 atcgctgtac ggtacggtat caccaatata gttgctttaa gagagttgag ccgttatgtt
    12721 gtaagcaatt tcggaagaag gtttagttat aactcccttc agaaagcact tggatttggt
    12781 agtgtaaaca cagtaaaatc gtatctgtcg taccttgaaa atgcattcct tctgaaaatc
    12841 gtatcaaaat atgaccattc actcaagaaa cagttgtcca atgagaaaaa aacatatatt
    12901 attgattcag ccattataaa actggtttca aatcaattga ccgaagatag agggcggctt
    12961 ttagaaaata tcgtggcgca acatctggct gttgataatc agatttatta tttctctgat
    13021 aaatacgaat gtgatttcat atcaatggat aatcacaaaa atattgctgc gtatcaggta
    13081 acctatattc tgaacgaaac caacagggag cgagagatta aggggctggt tgaagccatg
    13141 gattttttcg ggctgaacaa aggtgtcata gtaaccatgt cgcagagcga agaaataaaa
    13201 atatcaaaga agcaaataac cgttttaccc gcttggcgtg tgttattgaa ttacgcctaa
    13261 agtatatcgc taaggaaaac ggtgtttcat tcacattttt tgccaataat cacaattgct
    13321 attcatttca gttatacctg aatcttttta catatatcat tttagtttca gttataactg
    13381 tatctatgca aataaatttc aattgtttca tttatatctg aaactttttc ctttcatctt
    13441 tgcacctgga aatcagcaag aatcactaca catcattcaa taataacaag tcatttccca
    13501 aaatatgtat tatcaatgga tgaaggcttt gataccgaca aaaacggcat acgctggatg
    13561 aatatcaaag attttttgct ttcaaaatcg ctgctgtaat cacttccagc aaaccgaatc
    13621 cacccccagc acatagtttt cgatgacgcg tacggagaac actgtttcat cgccatcgaa
    13681 gccctcgata tcgacgtaat taatttgttt tttgtaggcc tgctgaaatt tatatgcaat
    13741 gggataatag ctccagtgcc attcttcggg ctgaaatccg gttttgcggg tttcaccaaa
    13801 aggtgtatat ggtctgcaaa aaccaaaatg cgccgcatgc gtttcgagcc attgcaacac
    13861 ttttatacca taggcagttt tgaaataagc tggatcgaca ctgttgaaat caatatcggt
    13921 accccagtgg tggcgcgatg ttccgggcat gctgctgtat ttcagaatga cttgtgctcg
    13981 ctccgtttcg tcgggataag ccgtgttgag cagctttcca cccacatact ggcgaccatt
    14041 ccacttgttt tcccagatca ttttctgatg ttcaaaagtg cgggtggctg acacaatcgt
    14101 cagtgtaatg ccatccttga gcgcactgtc gcgcatggct ataaaagcgt tgtacacttc
    14161 ggtacgtaaa taaatgcctt ttttcaaagt atatttgctg ctgatcaaag aaaaaccagc
    14221 ttcctgtgaa ggattgaatt ttcccagcag gaatcttttc tgaatggaat ctatagacac
    14281 cttatttaca agaactgagt caatacctga aacctgcgct acaagagaga aatgcagcac
    14341 taaaaatccg attatactgt ttattttttt cattttcgtt tcaatacctt caatgatttt
    14401 gccacaatat tttctgcact caatccatat ttctgcatca gctcgtcggg ctgtcccgat
    14461 tcaccaaagc agtcattcac tccaacaaat tcaaccggga caggatattc ttcagccaac
    14521 acttctgcaa cagctgatcc aaagccacct gcaatctgat gttcttcagc tgcaacgatg
    14581 gcaccggttt cgcgtgcagc atgaataatg atttcgcggt cgattggttt aatggtgtga
    14641 atattaatga ctctagtgct gatctgctga gcagccattt gctcggcggc ctgcagcgcg
    14701 tgccagacca tgtgaccagt tgcgacaata gttagatcgc tgccctcacg catcagctgt
    14761 ccttttccaa attgaaaacc atcaacttcg gatgtaaaat caggcaccgc ttcgcggcca
    14821 aatcggatat aaacaggccc tgaaagttca ttaacagcgc aaatggttgc caatcgggcc
    14881 tgtgtagcat cgcagggaga aatgacggac atattgggca gcacgcgcat aaccgcaata
    14941 tcctccaatg cctgatgtgt agcaccatcg gggccaaccg aaataccagc atgagcgcca
    15001 cctataataa catgtagatt gttgtaacac accgaaacgc ggatctgatc agcagcacgc
    15061 aaggtagaaa agacagcata tgtgctgaaa acagggatta atccggtaag agccaaacct
    15121 gaagccacag caacacaatt ttgctcagca attcccattg aaaaaaagcg atcggggaat
    15181 tccccggcaa aaaaattcat gctcaccgag ctggtaatat cggctcccag acccactacc
    15241 gacggattac gacgtgctgc atcgcgcact ccttcgccga atccgaagcg agtaggacgg
    15301 ttttccctgt taaaataaga cttcatttca tctgattaac agacaaaagt aaaaaattta
    15361 gagtcaataa tgcttcacac cagttaaatt gatgtatatt tgaaaatcaa aaaagaaaaa
    15421 acagaatatg gaaaacgttt ttcaaaacct gaagccagaa aaagtctggc aatattttgc
    15481 tgaaatatgt gaaattcctc atccttctaa gaaggaagaa aaaatggtca aatacctgct
    15541 gaattttggt aaacatcata atcttgaaac agtttcagac gaagttggca acgtggttat
    15601 ccgcaagcca gcgactccgg gatatgagaa caaaccatgg gttgctttgc agagtcatat
    15661 tgatatggta tgtgaaaaag aagccggcag cacgcataat tttgataccg atccgataat
    15721 tcctcgcatc gacggcgagt gggtaaaagc cacaggaacc actctgggcg ctgatgacgg
    15781 cataggtgta gctgcgcagt tggccttgct cgatgctaca gatattgaac acggaccaat
    15841 tgaatgtcta tttaccgttg atgaagaaac cggacttacc ggagcttttg cattaaagcc
    15901 ggaaatgctg aaaagccgca tcctgcttaa ccttgacagc gaagacgaag gagaaatatt
    15961 cattggctgt gccggtggaa aagatactgt tggaacacgc tattatacaa ctgaagcagc
    16021 tcccgaagga atgagtgcgg ttgaaattac cgtcggaggt cttaaaggcg gacattcagg
    16081 cgacgacatt caccgtgggc tgggcaatgc caataaaatt ttaggacggg cattgtggga
    16141 aataagtaac gatcaccctg ctgcacgttt ggtttcgttt gatggcggaa atcttcgcaa
    16201 tgccattgcc cgcgatgccg tggcggtttt tctgattcca tcaggaaaag aaaaagaggt
    16261 atttacatac attgaaaacc tggataaaac tgtcaaaaac gaattcaaaa ttactgatcc
    16321 gctggttcat gttgaaatca aaaaaaccaa taatcctggt caggttatcg acagcaaaac
    16381 agccaataaa ctgattgacc tgatatatgc catgcctcat ggagtcattg cctgggctca
    16441 ggacattgaa ggttttgttg agacatcaac caatctggca tcggtgaaaa tgagcaacaa
    16501 taccattacc atttccacca gtcagcgcag ttctgtggca tcggccaagc aaaacatctg
    16561 caatgccgtt gctgcttcat tccgactggc aggttttgat tacaagcatg gtgacggata
    16621 tccgggctgg acgccagatc caaatagtgc cattttgaaa atcacagttg agcaatttga
    16681 aaaacaattc ggatacaaac ctgtggttcg cgcaattcat gcaggtctcg aatgtggttt
    16741 gttcagcgaa aaaattccgg gaatggacat ggtgtccttt ggcccaacgc tgcgtggtgt
    16801 tcacagcccc gatgaaaaac tgcttattcc tacagttgaa cgattctgga atctcatctt
    16861 aaacatatta aaaaaccttt aaaacacaaa tcttatgaga aaactaatcc ttgctcttgc
    16921 attaaccggt gtaatgattt cacaaaccgg ttgttttggt gaattcggtt taacccgaaa
    16981 agtatatgaa ttcaatcagg gtgtgggcgg aaaatttgta aaccagattg ttttctgggc
    17041 tatgtgcgtt attccggtgt atgaagtagc cggtatgctc gatgtgctga ttttcaacac
    17101 cattgaattc tggactggca gcaatcctgt tgccatggct ccaggcgaaa ccgaagttca
    17161 gtatgcgaca gttgacggac atcagttccg catgactgct cagcagaatt atttcgttgt
    17221 cgaagagctg accgcaaacg gatatgaaat gagaaatgaa ttccgtttca ataaagatgc
    17281 aaatgaatta agcatcagca atggttcaga aacacaggta gttgccatga actaaacaag
    17341 tttaatgcta ctgaaaaggc tgttcgcaaa ctgtgggcgg cctttttttt gcgcaacgca
    17401 caacgatcaa tgcacaacgc gcaacgacat tgaagtcatc aagtttgtaa agtttgtaaa
    17461 gcaaaaaggc gacgttaatg cgtgcggcat gcggcatgcg gcatgcggag cacaacgcac
    17521 aatgctcaat gcacaatgcc ggcggctggt ctgcttacgc cttatgactt acgcctctca
    17581 accttcgtaa atttcgaagt gacctttaaa agtcagatag accaactgac aactgattgc
    17641 tgattgctga ttgctgattg ctgacaactg attgctgatt gctgattgct gactgctgct
    17701 aaaactgcgg tgtcacatag ttttcgggat gacaggcggt gcattccgcg aaaaactccg
    17761 acataatttt catatcagct gcataatcgc cgcttggaat aacaactttt ccaacaaacc
    17821 cgaatttctt tttataatca agataagtga gaatcatcgg tacattggcc tgcatggcga
    17881 tgcggtaaaa accttttttc caatgcggat ttggttttcg cgttccttcg ggcgtaatgc
    17941 ccagcacaag ctcatcgtat tttgcaaatt gagccgccat ttgttcggcg atgttgcccg
    18001 ggtttttgcg atcgaccgga acgccgcccc aagcgcggat tatgagattc agtggaaaaa
    18061 caaaagactc ttttttaatc atgaagcgaa cacggatatc gagcgcattg tagcacaggc
    18121 ggcccagaat aaaatcccag ttcgacgtgt gtggagcgac caccatgact gctttggtaa
    18181 tatcttttga tacagcataa ttatatttcc atccgaataa acggagaatt gctttagcca
    18241 aaataatacg gggccagcgg acccatttcc agcggttacg actttttgtt agtgccataa
    18301 tgcaaagata ggataataca atgattagta attttaataa aatgattagt tttgttacat
    18361 ccaaatatca aacacacatg aagcagattt ttctgattgt cgcattaata ctgtcttttc
    18421 agcaatattc tttttcccaa tccgatgaat atccgctcag cgaaagctgc acaagcatta
    18481 tggccggtaa aaatgccacc tccgacggat cagtcattac aagccacacg tgcgacgggc
    18541 gttatcgctc gtggctgaat attattcccg gggccaaata cgaagacaaa gccatgatgg
    18601 atattcagtg gggcaccatg atgaccgagt ctccatggga tacacgcgga gttgaaacca
    18661 aagggaaaat caaacaggca aaggaaactt ttgcttacct caatgtggcc tatccctgca
    18721 tgaacgaaaa gcagctcatc atcggcgaaa gcaccatatc aggacgcaag gagcttgtga
    18781 acaacaaagg actttttctg attgagaatc tggaagccat cgtgcttcaa cgctgcagca
    18841 ccgctcgtga tgcgataaat ctcattggcg aactggtgaa agaatacggt tatgctgatg
    18901 gcggagagtg catcaccatt gccgatccaa aagaagtctg ggtgcttgaa attttcggcg
    18961 aaggccctga caaaatcggc gcggtgtggg ctgcacaacg aattccggat gaccatgtgg
    19021 gtgtttgcgc caacatcagc cgcatcagcg aaatcgacct gaaaaacaaa gattatttca
    19081 tggcttcgga caatgttttc agcgttgcca aaaaaatggg attctgggac ggtaaggaaa
    19141 cttttaaatt ctggaaagcc tacagcggaa gcaaaccctt tcagatacgc gaatatttca
    19201 ttctttcaag tctggctcca tcactcaatc tcgactacaa tgccgaagag ctaccgttct
    19261 ctgtaaaacc cgacaacaaa gtgtcagtgc gcgatgtgat ggcactgctc cgctccactt
    19321 tcgaaggaac ccaatgggac atgacacaga atctgaaaat ggtcagcagt tataaaaatg
    19381 aagccgggga aacagtctcc gataccatta cttcgcccta cgccaacccc tggatgaagc
    19441 gcgaaatgat gggaatgctg aattatcaga atgacacttc agtgaaattt cagcgcacat
    19501 gcgccgttgc ctggtgtgcc tacagttttg tcgggcaatg ccgcgactgg ctccccgacg
    19561 aggttggcgg cgtttgctgg ttttcatttg ataatccggc cgagagtccg cgtatcccga
    19621 tttttgcggg tacactttca ttgccacaat cattcaatta ttccggacaa tttgccttca
    19681 acgaacaatc agctcactgg caatatcgtc aggccaaccg ccttgccact gtgcgctggg
    19741 gcgatggccg caagctgata gaaccggcac tgatgcaata cgaagacaaa gcatttgcag
    19801 aactggctga tgttgaaaaa cgcgccgctg aattgctgaa agaagatcag gccaacgctg
    19861 cagccggcca acccacacaa aaatgccgcg aatacctgac ccgctacacc gccgacttcc
    19921 acgcatcgtc cgctgcaaaa tggctcgagc tgcgcaatca gttgtggact gatttgaaat
    19981 tgtcgttttg atttaacaaa ccactcatag ggagtgaaat tttcgatggg gatagcgtaa
    20041 ttacaattct cattatcaat caaatacctt tagatttatt attatttccg taatttcacg
    20101 acattttatt gttttctttc cgttttttca tgacatttat tatatttata tctgttcttt
    20161 tatgatattt tatttgattt tattttgaaa ttcaatatat attttatatc tttgtccgat
    20221 aatataagaa gcattatgat cgaccaaaaa gaacttctgc aaagtctgat cctggatttt
    20281 cagaaaaaac tgccattaaa aatcttttcg cgcgacgaag agttgcccac tgacagcaaa
    20341 aaagtgatat cagtgtgcgg tgtacgtcgc tctgggaaaa catttgtgct gttcgacacc
    20401 atcaaccgat tgatcaaatc aggcgttccc aaaacagaga tattgttttt aagcttcgat
    20461 gatgagcggc tgcagctgaa gcgtcaggag ctcgatctca tacttcagtc ctatcgtgaa
    20521 ttgtatccga aaactgatat gcagaatgtt tacatctttt tcgacgaggt gcagatggcc
    20581 gatgggtggg aacaatttgt acggcgcatt tacgacagtg tcagtaaaca tatatttatc
    20641 agcggatcca attcaaagct gctggcaacc gatatagcca catcgctgcg ggggcgctcg
    20701 ttgcaattcg aagttttccc catgtcattt gccgagtatt gccgtttcag aaacatcgat
    20761 accaacaccc gggacaccga aacaaaagca caggtctatt ccggattcaa tgaatacatg
    20821 cgttttggcg gattcccaga actggtgatg aaccaatatc gtcatctacc gcaaacgata
    20881 caggagtatt atcatgtaat gctgtaccgc gacctgatag aacgatatca ggtgaaaaac
    20941 atacctgtgt tgaaatattt catctcacga ctgctggcca atcacaccag tcctacatcg
    21001 atcaataaaa tatacaatga aataaaatcg gcaggactga aaaccgataa aaatgtttta
    21061 tatgatttct cggagcagct cgaagccatc aattttactc aacgactgag gaaattcgac
    21121 aactcggttt taaaaacaga actggcaagt gaacggaagg tgtattttat cgataatggt
    21181 tttcttcagg cattgaaaca aaccaccagt aaagacaatg ataaattgct cgaaaacgct
    21241 gtgtttatgc attacagaag acaaacaccc ttcatgcgcg ggctctattt ctacaaaaaa
    21301 aacaaggagt gcgattttgt agtgctggac cgtgaaaagc catttgaaat aattcaatcg
    21361 tgctacgact tctcgtcgcc tgacacacgg aaaagagaaa tatcggcatt gttggaagca
    21421 tcggattatt tgaaatgcaa caatctgaaa atcgttacgc ttgatccgaa cgaagaaata
    21481 aaagcaggct caaaaaccat ccgggtgatc aatgccgccg agtttatgct gggccacaaa
    21541 cagaaataaa agcactacca cttcgcaggt ttttcatcct tcagaaggta atggattaca
    21601 ttttttcgaa cgaacaatag aatttgcgct catttaaaag ataatttcta tcattatcct
    21661 tgtaaatttg ttaactgaaa ttcaatccat atgaactcgg tcacttttca tcccatggtt
    21721 gtccacttct ccgttgcact ggtgattgtg ggctttgtta tcgatttatt ctacattttt
    21781 attgcaaaaa aagactggcg caaaaccagc agtctgttcc tgatgatact ggctgtgctg
    21841 gctgtcttct tttcatggtt cacaggcgaa tactactcgc gcagctacga tggagtcgga
    21901 cgtgatgtaa aagacgagca cgagctgtat gcatggatta ccatgggtgt attcggcgct
    21961 gcaacggctg tgcgttttat cgccatttat ctgaaaaaaa atcctgcatt tttcaaatat
    22021 ctttacattg ttcttatgct ttcagcagtg atggccattg gctattgcgg ctacctgggt
    22081 ggtaatctgg tgtatgagct tcagcacgat ttgcgtcccg gacaataatt ttcaacttat
    22141 tcagtaattc tgcatgtact ccattctctt cagacttctt ttcatcgctt cattgcttat
    22201 atcaagcatc aatacggtat ggtctcagga tattactttt caaatcagaa ataaatcaag
    22261 agagccatta attggctcca ccataaaact ttctgctgaa aaagattcaa caaaaatgtt
    22321 tctggccgcg gccgacgtga atggtgcagc tgttttgaaa aacattcccg atggcatgta
    22381 ccatgttgtt gtaacctaca tcggctacac tactttcgaa aaaaccatcg ctgtaaaccc
    22441 gcaacaccgc catttttcat tctcactgac agaaaattca ctggcactga acgaagttac
    22501 cattacggct gatcgcccat ttatccgcca ggaagacgat aaaatgattg tagatccaac
    22561 ccccatgatt ggcatcagca ccaacacact cgaagtgatt gaagccacac cgggcattta
    22621 tgttgattac gatgcaggca tatttttatc ttcaaccact ccggcagtca tatacattaa
    22681 cggtcgcgaa caaaaactca gcaatcagga tctgatgaat ctgttgcgca atctgccacc
    22741 gggtagcatt gaacgcattg aaatcctgcg cacgccctct acaaaatacg atgcagccag
    22801 ctctggcgga attgttaatg tagtattgaa aaaaggactg aaaatcggtc gcttcggaaa
    22861 tatcaatgtt gggatgaatc agggaaaata cggcaatcgc atagctggag caaatttcaa
    22921 caacagcggc gacaaaacca ccctgtatct gaatgtcggt ttcaaccgcc atgaccggct
    22981 cgacgaaatg caaaccgttc gtttaacagc aaccgacaca accttgaatc agcaaagcga
    23041 aacccgccag acatcggatg acttatttct gggatatggc ttaacatggg attcagacag
    23101 tctttccggg ttttcatacg acggtcgttt cagctttgga aacaggaatt cggatgtctt
    23161 caaccatagt ttttttgctg atgaaaccga ccagcatctg cttgaaagcc aggatcaaat
    23221 caacaccacc atcgaccgcc tgagtctttc gcaggacatc ggttacagct ataaaatcga
    23281 cacgcttgat tcggaatgtg atacacggct aggcttcgat atcagccgca acacatctgc
    23341 acagcatttc atttctgaat acacatttcc atatgatacc atgatttcag gattaatgaa
    23401 caatcatcag aatcgtatca tcattcaatt ggcgagtgat ctgaccctgc agctgaaaaa
    23461 cggaatcaaa acagaatcgg ggctaaaagg ctcatggcaa caatataaca gcaatgccga
    23521 cttcttcaca aattcaaatg ccattcaggt tgcagattca agccgcaaca acaattatac
    23581 tttcaacgaa agcatttttg ccgcttatac acaagcatcg aaaaccgttt ggggcggatt
    23641 ggtactgaag gccggctgcc ggctcgaata cacctacatg aacggacaac agaaacttcc
    23701 atatgacaca agctttctgg tcaatcgtgc cgatttgttt ccatatctgt acatcagccg
    23761 cgaccttcct accctgctac ccaacatcat tccgattaag ctcagaactt ttctgattta
    23821 ccggcgtacc attgcccggc ccgaatacgg acagctgaat ccctcgcacg aatatgttga
    23881 tccctattta tatgaggccg gaaatcctga tttaaagcca caattcacca ataatataga
    23941 gttcaatatc agctatcagg atatgcccat actggccttc ggcatcaatt acacacagga
    24001 tgttttttct gctgttgttt acgaagaccc caatcagaac ggaatagcaa gaatgactta
    24061 tgacaatctc ggcaaaaaca ccgaaaaata catgcgtgga atgattgggg tacctcccgg
    24121 aggaagatat ttttttgctt tgggcaccca gtacaatctg aacaattatg atggctttta
    24181 cgaaggcgag ccattgaaat atgaacgcgg cagctggcgt tttttcacgt ttcattcgct
    24241 caaactgaca caaaacacca aattcaatat cagtggcttt atgatggtta aagggtcacg
    24301 tggattttat gagctcggaa cttttggatc gctcaatgcc ggtttgtcac agacatttat
    24361 gaatcaaaaa ctgattgtta ccatcaatgc ccgcgatatt ctgggcacca tgaacatatc
    24421 cttcaatctg aatcagggct caataccctc atatggagag cgttaccgcg acagccaacg
    24481 cgtaggaatt aatatccgtt ttaatttcgg attacccaaa cgcgaaaaaa cgaatggatt
    24541 tgaggggttt gaagaggact aaaaaagagc ttttttactg acaatcaaat ttctacaagc
    24601 agaacagggc catcatttac ttttgcatta cagtgccgta catttgttta atatgtcata
    24661 aatagttttt tgacgaaata attatttcat ttaacggatt aattttatta tatttgtaat
    24721 acataaaaaa caggaggatt cattatgaaa gcatcagaaa agttgattat caccatcagt
    24781 cgtcagcttg gagcaggagg tgcatatata ggccaacaga ttgcccgtaa gctgaaaatt
    24841 cattatgccg accacgaaat catccgcaaa gcggccgaaa agctatcggt caaagaagaa
    24901 gacattgaaa gacgtgatga aaagactcag ccgttttggg aaaccatgtt gttcagttcg
    24961 ggttatgccc cggagtttta tgttccgatc aacataaaat atgcaccaac agatgccgaa
    25021 atgtataatg ttgaaatgag catcatgcaa aagctggctg aaaacatcga tgctgttatt
    25081 attggacgag gtggatttca tattttcaaa gatcgtccaa atacgctgaa agtttttctt
    25141 tttgcgccga aagaataccg catcgaccgg gtaatgaccg agcacaacct gaacgaagcc
    25201 gaagcaaaaa aagccatcga agacagtgac cgtgaaagag ccaaatatat tgatgctttt
    25261 acgggtctca aatggattga aagcaccaat ttcgatctga cactgaacac gtcaaaactt
    25321 ggccttgata agacagccga agccattctt gccatgatca atctgatgaa ataacagtgt
    25381 agtcagaaat atttttgtga tgaataaaaa ccggaattca ttcatatgtg atgatatttt
    25441 cagactgaat tttcatatac ataaataaaa aaggagttga ccatgaaaac atcagacccc
    25501 ctgataataa caatcagccg tcaaatgggc agcggtggct cgtatattgg gcaaaaactg
    25561 gccaaaaaac taaaaattca ttatgccgac cgcgaaataa tacgcaaagc tgccgaaaca
    25621 ttgtcggtgc ctgaagaaga tttggaaagc atggatgaaa aatctcagac atggtgggac
    25681 tacattcaac tttcgagtcg ctatgcatcg gaaatttata ttcctccagt gcagaaattt
    25741 gccccaaccg atcttgaatt atttaaagcc gaatgcgaaa tcatcaaaca gattgcaaaa
    25801 gaaacatcgg ctgtcatcat tggtcgctgc gggttttgtg tacttgacgg acatccgaac
    25861 ctgattaaaa ttttccttca tggagaaaaa gatttcagag taaaacgcat catggattta
    25921 tattcggttt ccatcaaaac agccgaaaaa atggttgagg acagcgacag aaaaagaggt
    25981 ttgtatatca agaaattttc aaaaaaagat tggatgaaca ttgataatta ccatctgtcg
    26041 gtcgatacca gcaaaattgg catagattcc tcattggatc tcatttcagc atttgttgcg
    26101 aacgccagaa aataaaaaac taatctgaaa ccccgattcg ggcaaacagg cattgattat
    26161 tgcctgatgg tcattttttt gcttagcaga tattgattta cagaaaaaca attaaaatgc
    26221 attttgtaat ttcgcgaaaa aatacaatga aatacctgtc agttcttttg cttgcatgta
    26281 gtattgcatc ctgtggaaac aaggataaac aaacagaagt tgtacgggaa ctcaatccaa
    26341 ccgacacgct tgacaagttg atgctaataa aaaacgaagc ggaactgatt gaaaaattcg
    26401 ggcaggaaaa tgtaacctgg gatacggtgt atggcgcaga aggagccgtt tccatggcaa
    26461 cactgctata caagggcact tgcgatcagg tagaaataac atgggaaaat ctgaaaaaca
    26521 aatcagccat tcagaaagta acattaaacg ccctgtacga tgaaaaacag gaaaagctga
    26581 ttgctttttc acattggaaa accagttatg gaattatgct gggaacttca ttgaaagaac
    26641 tggaaaccat aaacgaagct ccattcatat ttctgggttt cggttgggac tatggaggaa
    26701 ttgtaactga attaaacggt ggtaaactat ccaatcttcc tgtttcaatt cagcttggca
    26761 tttcagatat cgccaaacaa agcaataatc ctgactatga aatgctgatg ggtgataacg
    26821 aatttaattc aatcgatgct gaagcccagc gcctgaatcc ggttgttgtt tcaataagtg
    26881 ttttgaagaa ataatacatt tcgaaaaaat gaaaataaac aaaactgttg ccggtgtaat
    26941 agccggattt acagccggaa tcattgatct gattcctatg attattcaga aactcacctg
    27001 ggatgccaat ctggctgcat tctcaatgtg gctggcagtg ggctttttca cagcacacac
    27061 ttcattcagg atgcaaccag cattaaaggg aattttgatt gcctttattt gtcttctgcc
    27121 ttctgcattt attattggat ggagtatgcc tgaatcactc ataggggtag ccgccatgac
    27181 gctgattctg ggcgccctaa ccggcctgct cgtgcattgg atgacaaaag aaaaatcagc
    27241 gtaacccggg aatttgtccg actaatctat aatagaattg taagtccgat tcgggctccc
    27301 atgtaagtgc tcatggcatt gagctgcaga tcgagtccga atttctcatt gaaaaacata
    27361 cggtagccaa actgagcacc aaacacagca cttgtttcga caccaccatt gtataaagcc
    27421 ggtgactcag ccataacata accaccgctg aaaccgccat acaaatcggt gctggtatga
    27481 tcgcgcagct tgaacattgt gttgaaatga taatttacat tacccaacac agtgattata
    27541 ctgtgtttgt acataacatt tccgaagttt tcattaaaaa aacgataaga accatccaaa
    27601 cccatagaca gattttctgc aatgccaaaa tcagctccga gataagcagg aatgccctga
    27661 ttggtgtatc cggttccgaa attgaactgc agccggcccg cttcgagcgg atactgtgca
    27721 tcagccgacg aaaacacagt tgcaaaaaat acgatcagta ataatttctt catggtgaaa
    27781 tgcttttgta aaactgattt gtttgaatgg caaatataaa acatctgaaa gctgaatgca
    27841 aaaaaacaag aatttaattg tcctgcaatt ttaaatcttg tataattaag taatttgtat
    27901 atttttgtaa tgaaatacta aaaaccaaat ccatgagcat aaaaggaaca aaaacagaac
    27961 agaatctgct gaaagcattt gcaggtgaat cacaggctgc acgccgctat aaaatgtttg
    28021 ccaaaatagc aatcaaagaa ggatatcaac aaatcgccgc tattttcgaa gaaactgcta
    28081 gtcaggaaga tcagcacagt aagcgctttt tcgagtttct cgaaggtggc atggttgaaa
    28141 tcaccgctgc ttatcccgct ggcgttctag gcaccacagc tgaaaacctc gaagcagctg
    28201 ccagtggcga aaacgaagaa tggaccgagc tttatcctga atttgcgaaa attgcagaag
    28261 aagaaggatt caagaaaatt gccaccgctt ttaaaatgat tgccacagtt gaagctcagc
    28321 atgaaaaacg atatcgcaaa ctactgaaaa acgtgattga cggtaaagtg tttgaagagg
    28381 aagaagaagt ggaatggatt tgccgtaaat gtggctatgt acacaaaggc aagaaagcgc
    28441 tgaaaaactg ccctgcatgc gaacacccga tagattatca ggaacgcaaa gcaagcaatt
    28501 attaaaataa tgaactttgc attttcaggg aaaaggatga aatatttcct tttccttttt
    28561 tttattgtga caactattgc tttctgcttc gggtcgccct accctgaact gatgatgctt
    28621 catcactcgg caacaattct gacagcagct ttccttgttt atattatact aaaaaacaat
    28681 ctgagcaatc tgtcttttct ttttattatt ctgtttattc tgctgcatgt aatcggcgcc
    28741 aaatggacat atctcgatgt gccctatgac caatggtgga attggctcag tggcgattca
    28801 ctttcttcgg tttttggctg ggaacgaaat caatatgata gacttgtcca ttttgggttc
    28861 gggcttctga tatggttgcc ggcatacgaa atactgcaac gttggtttgg ttacaaaaaa
    28921 actcatgcgc gctacgtagc atgggaattc attgccatgg caagcgtagt ttatgaactg
    28981 tttgaatggt tgctcacact tgttgctccg gctaatcagg ccgacaatta caatgggcag
    29041 caaggcgata tgtgggatgc ccagaaagat atggcgctcg ccttactggg agcatcaatg
    29101 gcctggttgg cttttttgat aaaaaaatgg tactcatcgc gcaatcaatc attgattagc
    29161 aaacgctatt tatagcccct tccggattga tatcagccgg agcccaatca atgaccggca
    29221 cgtcaaaata ctggtctgaa aaatagcagt gattatcggg tctttcaccc attcggactg
    29281 tgtattgaga ataaaaccaa actttttcag ttttttttct gaattatttg gtcaaatgta
    29341 aaaaaaacgc tgtatatttg cactttcttt taaaaaagca aaattccagg ccatgaataa
    29401 gggagaactg ataaaattcg tagagaacca actcattgac agcaaacaat ttccgatgtt
    29461 taaatcggga gatactgtca ccatcaagta taaaattact gagggaaaca aagaacgtat
    29521 ccaggctttt cagggtattg ttcttcagat ttcgggcaaa ggcgccaaca aaacctttac
    29581 tgttcgtaaa atttcgaaca atatcggtgt tgaacgtatt ttcccattct caagcccttt
    29641 cattgatggt atcgatgtaa acaaacacgg cgttgtacgt cgttcacgta ttttctacct
    29701 tcgcgaactg cgcggcaaaa aagcccgtat caaggagaaa aaatactaag atatttttat
    29761 gctttgcaaa aagccggcaa ttgccggctt tttttgttga ttttaaactg catgcctaat
    29821 acagtgcgaa aatcagagcg agagcgatgt ttggtcatgc gctatactcc atgctatacg
    29881 tcgcctttaa aaactcctgt gaataaaacc gcgtaaaacc cggcaatctg taatgaatcg
    29941 gcacaatcgg cattagacga aagacattgt atgctgcgca ttgtgcgtta tgcaaaacta
    30001 tttcaaccca atacgcatca cttcaaatcc ggtcggacga taaattacgg catcatcggc
    30061 tgacgttgaa aaatctactt ttatttcctg tccgggcttc agctgaataa tttcaatccg
    30121 ggtttcacct gccagcaaat aataatccct gaccgtatat gatgggattc ctgtatttcc
    30181 gaccagagtg agcgaaacat cattgccatt tacaattaga tctttcaaaa taatgggtga
    30241 actgatttcc ttcatcacat catacgatgg ctttggattc agctttacat cgcatattcc
    30301 atgaatccgt tgcggatagt gataggtcga gtcctcgccg aaatgagtac gataatcgtt
    30361 cagacaaaaa taaatagctc ctgcaacata ctccttgctg ccatagattt taatttgctc
    30421 cgccatttcc atagccctgc gctcatcgcc acccttaaac ttcggctcac atattcccca
    30481 ctccgaaata gtaatgggct tttcaggata caactgatgg attctgtcga gtgcaagcgg
    30541 aattgaatcg agattgtatc cgtaccatgt agtgttgtac tcgttgtaca taatcatgtc
    30601 gtagcgcggc gatgcatccg gtaaaatatt tttcggtccc ggcacttccc aatgaacagc
    30661 attggaaata taggccacca agcgcgaatg atcaagactt cgagcatgtt tttccatggc
    30721 atccaatccg ttgatattaa cttcttcgtg acttgagagt tcgtttccaa gcccccagat
    30781 aataatgctt ggatgattgt aatgcgcttc aatcatttca tcgagatgct gtattgcaac
    30841 aggaagcaaa gagtcgttaa gatgcggttc ccacccccat agcggtactt cttccatgac
    30901 cagaattccg ttgcggtcgc accaatcgaa aaacttttcg ctttgctgcc agtgacttcg
    30961 ggcaaaaaca caattggcat ttttcatcaa cgtgaggcct ttaatcaggt tttcatctgt
    31021 ttgagcaaga ccatgattca tgtcttcacc cacagtccat tccatgccca taagccgcat
    31081 gggctcacca ttcagcacat agcgattgtt ttcgattgta atgctgcgaa atccaaacac
    31141 agcgtgcaat tcatctttcg ctgttttccc ttcaaacatg gtgatgtcga tacgatacaa
    31201 atccggagaa tcgaaatgcc accgattcac tttcttcaga ttaagtgtac tcagaaaacg
    31261 accatgacta aactttccct tcaattttcc tttaaaaacg acattgcgtg ttttttgatt
    31321 ttcttcggta atcacagcga tgaaatatgt tttgctgcca ttgatttttg aaaaatcaat
    31381 caatggaatt gaaatttctg cgcgaccacc atttccttcg ggaataccgt tcacatgaat
    31441 ggtgcgcatg gcctgttctt cggtaaaaat aagatttacg ctgcgaataa ttccaccatc
    31501 gtgcgaccaa tcgaaacttt tcagataagg gatgcttttt ttcgatggct cattgttggt
    31561 ataaactgtt acaatatttt cgcctggttg gatgtatgga gtgatatcga ttgaaaaagg
    31621 cgtgtatcct gagccaaagt gctcaccagc ttttttaccg tttatccaga tgatggcatc
    31681 gtggtttacc gcttcaaacc tcatctgtac ggtttttttc atctgattct gagtaatctg
    31741 aaatttcctg tcataccatc cggcacccca atataaataa tatccttcgt tggtgttgaa
    31801 agtgtgcgga acaatggcaa gccgctttcc gcgcggagga agacctttct cgccccaacc
    31861 tagctgaagt ccgcgggcat cgggatccac tacaaaattc cactcaccat caagcgaaac
    31921 catatctctg ttttgtgcag aaacattggt tggttgaacc aggaaaaaaa gaaaaacgac
    31981 catgcaagag cggaataaat gtttcatttt taatggggct tagaaaaaat tagtaacagc
    32041 aaacctacaa ttttccaaaa caaaatatca ataaaacaaa agagctatcc gttatttttt
    32101 gacgaacagc cctttgaata gtttttttta tttcagtttc cagattccgg taattgctcc
    32161 ttaagctttt caacttccat cagacgcaga tttaattcat tgatgtaagc tctttcctta
    32221 cggagaccaa aatccttata agctttattt gcccattcca gagcgataga caattcacct
    32281 tccatttcgg cagccagagc catattatga caagcgcgac cagcgatttt gggatcaggg
    32341 cttttggccg attgcgacca gagcaccaca gcttctttcc aattgttgac ctttacaaaa
    32401 cgtttggcac ttttaaggcg atcatcacct tttttataat aaatacgcga aacacgcacc
    32461 caattgggcg aaatacgaat acccatcatt tcaccggcaa aatatccggc atcattgatg
    32521 gcgttgcgtt ttgacggcaa ctttctaaaa gcatcttcag gtgttggtcc aacagtactc
    32581 cagcccttat catcgtaaaa aacatcctgg tcaatgattt ttttattcac attgtcataa
    32641 attcgccatc cggcattcac cctgatgttc agatcctcga taaactcggt taccatcaca
    32701 ttttcgccat taacagcgcg ttcaacctgc ctgctgcgcg atacacgacg gatatcactg
    32761 tcgaaagtct cgagcgaaac aatggcatcg acgttatatt ttttgcagag tgcatcaact
    32821 tc
//
LOCUS       DAOPKE010000025        32413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1460_length_32413_cov_13.522436, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000025 DAOPKE010000000
VERSION     DAOPKE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_32413_cov_13.522436"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKY63_04985"
     CDS             <1..913
                     /locus_tag="PKY63_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPF00006.1"
                     /translation="LNLNLNLNLNLNLKLSLMKYFTAIFLLISLHSLAQKPAYVLSNP
                     SGKEIGWTAFVDQLSAADIVFIGEYHNNPICHWMEYEITAALHEKKAGKIILGAEMFE
                     SDNQLLIDEYLAGKIKQKNFEDEAKLWNNYQTDYKPLIEFAKEKGLLFVATNIPRRYA
                     SMVAAGGFDALDSLTAEAKKLIAPLPMAYDENLNCYKSMMSMGGMGGGHANANLPKAQ
                     AAKDATMAHFILKYFKPEYTFIHYNGSFHSDYSEGIAWYIKKENPAMKIIVISVSEVD
                     DVLKPAPEDLNKGDYTILVPATMTKTY"
     gene            complement(1100..2305)
                     /locus_tag="PKY63_04990"
     CDS             complement(1100..2305)
                     /locus_tag="PKY63_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF00007.1"
                     /translation="METELIIREKYLNKVKPFIQKPIIKVFTGQRRVGKSYIMRQTIQ
                     QIEKKDKRGKIIYINKENVEFNSIATSTDLLEYISQHSTKSIRNYIFIDEIQEIKDFE
                     KGMRSLLLNPAYDLYCTGSNANMLSGELSTLLSGRYIEIPVYSLSYPEFLIFHGLKNQ
                     EASMKKYLKYGGMPFLKHLELKDEIVYDYLKGILSSVMFRDVILRNKIRNSAFLENLI
                     EFLADNTGQLFSARKISEYLKSQRIQITTAQIIKYIHYLTSSFFINKVQRQDIVGKKI
                     FEIGEKYYFEDVGIRNTISNYKQTDIGKIIENVVYKHLKYNNFEVKVGQTGPYEIDFV
                     AEKQGEKLYIQTCYLLHDQATIDREFGNLERIKDNYPKMVISMDQFTGNTFNGIQHLQ
                     LRDFLSMEL"
     gene            2493..3128
                     /locus_tag="PKY63_04995"
     CDS             2493..3128
                     /locus_tag="PKY63_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00008.1"
                     /translation="MKELRQFGSFPFTHSNLLELLHGYNSPGDKISSLIKNGDIISLK
                     RGLYINAPKYRSSMVSKFQLANVIYGPSYVSMESALSEYGMIPEGVFTTRSVTLKRSR
                     NFVNSLGKFEYFHSPENFYSIGIGISNRQKEGSCLLASPEKALCDKIVFSKNSTIQSE
                     KEMLNYLEDDLRIDMNLLKDLDEAVINLCAAAGRKQALLELVLKIIFKLKR"
     gene            3125..3952
                     /locus_tag="PKY63_05000"
     CDS             3125..3952
                     /locus_tag="PKY63_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF00009.1"
                     /translation="MSLLNELILQHNPSDENEWKQAMRESMQIIALSGLYRGNFFENA
                     AFYGGTCLRIFHGLERFSEDMDFSLLHADENFSLEKWFDHLQLEFKAAGTTIEIERKS
                     KTQKSMIESAFLKSDTKEYALHLKSEKHLKIKIEVDKNPPLRFETENKLLLQPFSFFV
                     QSFTLPSLFAGKMHALLFRSWKNRVKGRDWYDFEWYIRKGVALDLQHFNERSVQSGHL
                     QNYIDRQQLHKLLINKINVLNFKSAKDDVGPFLKSSDNLEIWSPEYFIALVEKMKIA"
     gene            4057..5745
                     /locus_tag="PKY63_05005"
     CDS             4057..5745
                     /locus_tag="PKY63_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPF00010.1"
                     /translation="MSFRELFRRKSVADIVAQHEKDDKPMKRTLGVVDLTALGIAAII
                     GAGIFGTIGKAAVEGGPSVSLLFVITAIACGFSAMAYAQFASSIPISGSAYTYAYASF
                     GELFAWIIGWDLIMEYAIGNIAVAISWSDYFTGFLTGFGWHLPEFMTVDFLTASRAYE
                     TVTQLLSDGTIFSSIDAPLREGYLAWKSAPQIGDVHLIADIPAFCIVALISILVFRGI
                     RESKIASNLMVGLKLIILMVVIAVGAFYVNPDNWSPFAPNGFSGMMKGVAGVFFAYIG
                     FDAISTTAEECKNPKRDLPRSMMYALGITTAIYIIIALILTGMVPYDKLGVGDPLAYV
                     FEQAGMPHLSGIIALSALIAMAGVLLVFQVGQPRIWMSMSRDGLLPKRFSKLHPKYKT
                     PAFSTVITGIMVAVPALFTNLTEMTDLTSIGTLFAFVLVTGGVILLDKSDPEIRKGFR
                     IPYMNSRVWVPILLVPVYIALFMFTSDFSLEAFMGERIPVLIYFGVAIVVMIAAVIRQ
                     WSFIPVVGLLINLYLMSELGVTNWLRFFIWLAIGLVIYFTFGRSHSKLRKPDGN"
     gene            5735..6523
                     /locus_tag="PKY63_05010"
     CDS             5735..6523
                     /locus_tag="PKY63_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF00011.1"
                     /translation="METSVVIILICSGLIVGFINTMAGGATIISLSVLTWLGLPLTVA
                     NGTNRLAVFFQTITSVSNFQNSHLIDWKKSIRVGIPTVIGSVLGSFIAVKLNEDIIRY
                     SFVVIMIIMLAFILIKPSLWLKGNAALLELPVKKWNYPLFFIIGVYGGFLHVGVGYYL
                     LATVVLGLGLDLMRANVMKNLLVMLYVPFSLVLFIVNDEIAWKYGLIHAIGNVIGAFV
                     ASRIAIKRGADVIRWIMIVVIALLTADMAGLINIKSAIGVLLAK"
     gene            6560..7180
                     /locus_tag="PKY63_05015"
     CDS             6560..7180
                     /locus_tag="PKY63_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00012.1"
                     /translation="MGKGGFLRRWRLQYYYFKRTGFYTEVGKGLINFILIFAVVVGLV
                     IIAEKTILDVDAVFNYVVTHVPNWLVFLVAFSTELLFGLIPSDLFIIWSEGHDYPWVA
                     LTILALMSYLAGILTFSFGKLIRKNKKVNSYFTSRFEKHIRNTRKWGGWLVAAAALTP
                     LPFTLIMVVVGMINYPIKRMMLWSSLRIPRYFLMALIYYHTINLGL"
     gene            7300..10974
                     /locus_tag="PKY63_05020"
     CDS             7300..10974
                     /locus_tag="PKY63_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF00013.1"
                     /translation="MILKKIRYFSPLLFLFISALLLFLAYSFSGGKSIKSEKQINAFK
                     TSLEKAVTETDSLLNWASNPMFLNDPFSILSVGSFVDKTRSLNATVFISHNDSLIFWS
                     DNRTPLPPDIDTLQSGNLVQSGNGVYLLRAFHEGSYRFFSLMLLQSSYFYQNNYLISG
                     VNPCLCDDCRFQISSDQTMADTLIRVDGVSPLMPLVFSKDDISLSSAQDVAVVLFLFF
                     ALVFLAMAFYGLASRLALLKHTTWLRLVYFALIPLVLYFFGQYLFSLNLFDQLSVNNP
                     AFFAYSGRFPNLTPLLLYAFLFVLWISFFSHELLLIAGRKRKNVFQRLAFLFITYITT
                     ATLAYFVISGINSFLFNTSINFTFNNVLAIDIRSVLLLLTLFLLFCGIILLHYLMISL
                     VSRLRLSFKTWLVVSVVFLLIGALTALIAGFNVWLFVSVSVYLLLMQFVFSRHMYSNR
                     TVYLLLNIALAAGISTVSLNYAIQQKQYDQLKLLSLNLATEQDPLTEEFLGDLIPQIS
                     QDSILLNDPYRMIEEFNLTQYLKRKYFNGYLGQYILQTTWCRPGEEILVQPSDTAISC
                     IGFFDDVAFRMGIPALSDNVVFVNDGTGRGYYLCKIRLDAPGDTSYIFAELIPASYEK
                     GLGYPDLLVDVNSGIKTVPSNIAYARYLDDGLVSRYGKFNYPTRISALFNTMPDEKVI
                     HDHGYIHFKLTISDRLVLIITESDRDIPSSLAPFSYILLFFGVLVVLFLLIAGRVFGF
                     RLRFAVSFSSRLQWMIILIILVIFFVAGVISVLNLQSLNKSKNEEIVSEKAHSLVVEL
                     EHKLSQYGPLSFADEQYVTELLLKFSQVFFTDLNVYSLNGKLLATTRPQIFDKELISV
                     RMNADAYAKLSQRNHSFLLITEHIGEMAFMSVYIPVRNDMNENIAYLNLPYFARENEL
                     SREISSFISTFINIYMLIILLTVLITVVVSNYVTQPLRLIKDKLRNIKLGTLNEKIKW
                     KRRDEIGDLVFEYNRMIEELAYKADLLARSEREMAWREMAKQVAHEIKNPLTPMKLST
                     QYLEKAWNDNAEDFDERLKRYSQIMVEQIDSLSDIATAFSHFGKMPESTVVKICLNDV
                     LSSVITLYRSEDYDITLQVPDTALYVFADESQMLRVFNNLLKNAQQAFVPARRGMIHV
                     TLKVEENKALVVVQDNGAGMSEEQKKKIFQPNFTTKSSGTGLGLAMVKNIVSGFGGRI
                     WFESVENVGTTFSVELPLAE"
     gene            complement(11051..12709)
                     /locus_tag="PKY63_05025"
     CDS             complement(11051..12709)
                     /locus_tag="PKY63_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPF00014.1"
                     /translation="MVKFKKLKDYFNNIIVRAEQNKLLCFVYVLGMLFLINACATVEK
                     PNGGPADRTPPQVFSATPADSSIHTNNKLIILEFNEFIKLNNPNSQIIINPFPKEKPE
                     FSVQGKKVKIELKDSLLPNTTYHIFFGNAIQDITENNPAAGLDYVFSTGSYLDSCTLS
                     GFAGDAFTNKFTDKAWVMLYKSLHDFRDTLPVYIAHVDAKGAFKFRNISAGSYYLCAL
                     EDNNSNFRFDIPDEKIAFSNNPYLLTAEKPTIDSVNLYLFTEKANTQKHLKTEMIHYH
                     TVRSSFRMSFEHPQITILNKKGETTFEWNEKKDTVIFYIKGPDADSLRYIIRDGAFSD
                     TLYQALKYKGRQKDFSTDTLFRISSTKGEKITPNESISISSIIPIEHWDFSKTLLAIN
                     KDTLPVSMEKSDETGRQWTLLNKLKPGENAVLIIKKGAATNIFGYKNDSIKLAFRMIQ
                     ESDLGSLQIDLMNLPGKPLIVSASSKEKTYTLISSAEKEQIVFKNMLPGEYNLKIIID
                     ENEDGEWTPGIFDQKKQAEKVFTLPRPVQIKKNWESKIGWNLTY"
     gene            complement(12714..14012)
                     /locus_tag="PKY63_05030"
     CDS             complement(12714..14012)
                     /locus_tag="PKY63_05030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPF00015.1"
                     /translation="MKTKADVNLVKNFISEKDFSDIEPLVQKAREMLLGKTGSGAEFT
                     GWVNLPSSLLENKDILNKIEATCHRVSTFNPDLIVVIGIGGSYLGARAVYDALSPSIG
                     HRKPEMIFAGHQLDSAYHAMLMVYLKERNPVIVVISKSGTTTEPAIAFRLIRTLMEEK
                     YGSDAAQRIIAVTDEKKGALRTLAVAKGYETYIIPDDIGGRFSVLTPVGLLPLALCGF
                     SIQDLLNGAAECQKELTTNHTLANNQAMLYAAIRQLLYRQGKHIELQSTFCPKLSTLS
                     EWWKQLFGESEGKELKGIFPASLVFTTDLHSMGQFVQDGNRVLFETFLSVAKPEKDIE
                     IPVLNSNDDELLYLEGKQMSYVNRKAEEATRIAHAQGGVPVLALEMENLTEFSLGEIM
                     YFFEFSCALSAYIQGVNPFDQPGVEAYKQKMFELLEKNNK"
     gene            14231..15910
                     /locus_tag="PKY63_05035"
     CDS             14231..15910
                     /locus_tag="PKY63_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF00016.1"
                     /translation="MTKETRLKTIIIAVSAVALIGIASIQYYYIRTAVHASEVTFDRN
                     VNDVMSKIIFQMEKNEVASQVRSKLKSFSREQQLINTLDSLNQELFRSLQKIGIDSMR
                     TDSIIRQTRNEISEKVAMNQYGEYLKNVDTSKIKKDVVIPRADSLLWMELPFALKEKK
                     ARADAHKNLKTIQEKRLDELYRNVDRFLRRTYLIGDVMEDFFNINHFFPVESRIDSAN
                     LDSMIRSELAFRGITAEVVFGIYSTNRDTIILQSVPGFEERLRNSNFGYKLFPSDMFS
                     SPDYLMLYFPHKRRYIYSEVSGMLMLSLFLVGVIAVSFFFILFNLLRQKRLSEMKTDF
                     INNMTHEIKTPISTISLACEMLADQSASIDEEQRSGFIQIIADENKRLAAMAEKILQT
                     AIIDQGRMDLKKEKTDIHRLIEKSVNNIKLWVNQKDGFVDVNLRAAHFFADVDPVHFE
                     NVIFNLLDNAIKYSPYHPQIFIGTENSNGHIVVTVRDNGIGIEKKELKKIFDSLYRVP
                     TGNIHNVKGFGLGLCYVKAIISSHEGTINVESEVGRGTVFTIKIPVLNEKQ"
     gene            15897..16592
                     /locus_tag="PKY63_05040"
     CDS             15897..16592
                     /locus_tag="PKY63_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF00017.1"
                     /translation="MKNNKIFILLAEDDVNMGLILQSFLTAKGFEVLLARNGVEALSL
                     FGRNAGIDMALVDVMMPEKDGFSLAADLKATSPSLPLVFLTAKSMQDDILKGFEIGAD
                     DYITKPFSMDVLLARITALINRTVNIQKDSDQPHQLGKLSFDFSRQTISDGKDVVKMT
                     SKEAQLLKLLAENKNSITDRKLALEMIWGNDDYYSSRSMDVYITKLRKMLKMDKSIEL
                     INVHGQGYKLMCS"
     gene            16622..17149
                     /locus_tag="PKY63_05045"
     CDS             16622..17149
                     /locus_tag="PKY63_05045"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPF00018.1"
                     /translation="MSEKIQLYSASEFMTIANEYCWLMENIAGFESEKVLDFMRKILP
                     LLYVKGCAVVVPEGADETDMQRYVTEENYEIIFNETRNKLKEYDVFFIFNNDLKEPEE
                     RSIAEGLTDIYQDMKDVMIAYNKGLEPEKAAALYCLKMWFIDRWGKHAADLLPVFHNL
                     YEKKHSNQQTGTEFD"
     gene            17159..>18468
                     /locus_tag="PKY63_05050"
     CDS             17159..>18468
                     /locus_tag="PKY63_05050"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HPF00019.1"
                     /translation="MKKILVFVVLSLTVLSLSLAQGYKISVKINGLSEDTLLMGYYYG
                     KYQYVRDTIVLDKTGKGVFDTKDTVGGGIYFIVLPDKNYIEFVLDKERDFSIEADTSD
                     LVKSVRAKGSSDNASWFAYKVFIADKGAEIDKLGKVHKRLQATGKEDSVKILEQQMKD
                     INAEVADYKNKFIKDNPKSLVSIIFDCSREPDIPEAPMLPNGRKDSTFAYRYFRAHFW
                     DQYDFTDDRLLRTPIYHAKLDQFLTKVVPQIPDTLIAEIDVIIEKTRSNKEVFKYTVW
                     YLTHYYETSQVMGMDAVFVFLADKVYRAGDAFWVSDNVNKKIIEHADKLKPNLIGKTA
                     PELVLLGLNNQLISMHSIPATYTILYFWDPSCGHCKTETPKLINYINKVKDTLDIKVY
                     SVCSDTSLTAWRNYVAEKKMEVFINVNGTRTAKGNFHDLYDIFST"
     assembly_gap    18469..18568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18742..19641
                     /locus_tag="PKY63_05055"
     CDS             18742..19641
                     /locus_tag="PKY63_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF00020.1"
                     /translation="MSNSFSRRIRNQGIAFAMTDVLAMTGVYIFSKIFLNDLPFPVFG
                     VYWFSAGLIWNLILSFFVPGTYSKFEIKGSYLVFLVVVGILDMAATILWFEAVDKTDN
                     PSLVSFMTNISPVYAVILGYVFLKERLSLSEGIGIIVTLAGAVLIGYRNDFTLNSFLF
                     SGAGIILISSLISQGGKVILKTRIRNYHPIVLSINRIVFLLLFSFSFVFIFDYSLNIG
                     SEMIVYIAIGALMGPVISAFAGYNALARLKVSTWSILSTSRSFFVMLVSYLILNNLPD
                     TIQIAGGLLTVAGVVVISLARVK"
     gene            complement(19958..20566)
                     /locus_tag="PKY63_05060"
     CDS             complement(19958..20566)
                     /locus_tag="PKY63_05060"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPF00021.1"
                     /translation="MSITLILIIVTVAVSLIAFSNRVLFAHLLFYPWEIKRENQWYRF
                     ITHGFIHADFMHLFINMWVLYIFGEVVEKSLKFIYGSEGTLYFMLLYFGGLIFSSMYS
                     YGRHLNNPQYSALGASGAVSGITFAYILMYPMSGLMIFPIPIQLPAFIFGILYLIYSW
                     YMGRRGKDNIGHDAHFFGALYGVIFMIILDYRFVTLFLSNFR"
     gene            complement(20570..21376)
                     /locus_tag="PKY63_05065"
     CDS             complement(20570..21376)
                     /locus_tag="PKY63_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00022.1"
                     /translation="MKSLWLWILAIILAVSVMVYQRMTGPTTPVRGSVEYLGETVKYK
                     MLRTWGGSDGARIEVKTNHPDAAGVVQYRRFRSNDEWKVLVLKNDNGLLSAQLPALPP
                     AGKMMYQVFLFERNNHVALTEDPVVLRYKGEVPAGVLIPHVIFMILSMVFCIRAGLEA
                     IKRRKQLVSLTFWSMLTLLIGGLILGPIVQKYAFDAYWTGWPFGHDLTDNKTAISFIL
                     WVVAYFIIRKNPQKRGWVLAACILQLAVYLIPHSVLGSEIDFTQPQYHQH"
     gene            21444..22883
                     /locus_tag="PKY63_05070"
     CDS             21444..22883
                     /locus_tag="PKY63_05070"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPF00023.1"
                     /translation="MKMLRFIFPVLSLCVLLLAAGCRKDEQFNDDPSFRLEFSADTMY
                     FDTVFTTVGSVTLLARVFNNSNDFVNISSISLGGGTNSNFRINVNGLEGTSFTDIEIG
                     PKDSIFIFAEVTVDPTNSNNPVVIEDSINFIINGNHQKIQLVACGQDACFHFPDHPET
                     EYLPAYSLVSGVWNSDKPHVVYGYAVVDEDSVLTISAGTQVYMHNNAVLWVYDGGTLQ
                     VYGAKDNEVTFQGDRLDDYYKDLPGMWGKIWLSAGSKDNIIDYAIIKNGRIGVHVDTL
                     GASVNPTLTMTNTIIDNMSTACFVAQGSYVKAENCVFGNAGYFSVLLNIGGNYDFRHC
                     TMGNYWSHGTRSASAIVLNNYYEDIYGNTQIRDLTNAYFGNCILYGANEDELLLDKYA
                     SGGVFNFQFDHCLLKTSMSVSGSGYTDCIRNQNPAFPNTQLPDFSIVSGSAAIDAGDA
                     AVTGTIMYDILGNPRIAGTAPDMGAYELQ"
     gene            23286..29429
                     /locus_tag="PKY63_05075"
     CDS             23286..29429
                     /locus_tag="PKY63_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPF00024.1"
                     /translation="MKNTFLFMSLIILFSALPAFSQNDAAYEKLWKTTDSLIGKSLPQ
                     SALAEVEKIYAKATAEKNNAQIIKSVIYRITLMSQYQEDYYENSIAEMKKEIEKAEFP
                     VKPLMYAMLGEMYWNYYTVNRYKFYQRTATDNFDNEDIKTWTLDRLVTEVKWCYDQAL
                     KEPQKLADVKLNTYQAIIDYYDYLQYSPTMLDLIGWMAITRFSNEETALTKPARVFKI
                     DNKDYFFPGRSFAGMKVADYDTTSFVYYATKVFQTLTKLHEKDADSEVFTFVELQRLA
                     FMRSKCTLPNKDTLYLNALNELHAANAGKKGRAETGYALAVYYNSLASRYHFETAPQY
                     KWYNKTALEIIEETIRDYPGTRGANNCNVLKETILTPAITLNFENTSYPGKPFEGILS
                     YSNINNAYFRVIKIDHDKDRELDKKLSYEEIIEYYAKLTPVKEFSVMLTDPGDYQNHS
                     TNVVFDGLSAGYYIVLHSSTNDFSMAKGWVGYTSLWATSISYLMQDYNNQETTFLVTD
                     RLSGKPLSGAQISVFYEKYNYITRDYVYNKYKVLTTDASGKAYLGTLPKGSDYRYMYA
                     DVSFKEDRYCPYSTFYMYPPYDYSNNGQTVSYFFTDRSLYRPGQTVYFKGIMLKTDKD
                     NNYSILPGTKSTVTFYDANYQVVSQVELTSNDFGTFTGSFTAPDNGITGSMRITNGYG
                     TAYISVEEYKRPKFYVQFDPVKGSYKLNEKVSVSGKALAYAGNNIDGAKVKYRVVRNA
                     RFPYWWGWWSYTMPYSAQVEITNGVTTTDENGVFKIDFTALPDESVRKDYLPVFNYTI
                     YADVTDINGETRSGNTWVGAGYKALILGSNLSQDIVKGTVENYEISSENLSGQTVPSD
                     VTMLIYTLKSPDRVMSQRLLAQPEYKSLSKEEYLKMFPFGVFDNENNPSEWEKGKLVY
                     SHLYNTATDSLVNIDDVLGGMQGAFMMVLSAKDSFGENVEYQRFFNYYDPAEKKSCSN
                     QVFYVHEVNTTCEPGETASILVGSFGEDVKILVEVEHKNKIVSAQWYTLNKEQKLLTF
                     PVTEAHRGNFAIHLVCVKGNRFYSGNVNVYVPYTDKQLDIEIESFRDKLQPGEGEKWK
                     IIIKNAKGEKELAELMAGMYDASLDAYKSHSWYLNLLSYYYGNRSWDAASNFTTKYSY
                     QYSLAYSRKYYNQMIYTPKLRWFNYPLYSYNSYYYSYDDEDYGDIGVLSCCQTAATGT
                     TREKASAKKDAPAAMDVLEAEEMLMSDGEVDKDAEVTKAGLLAGNDMRNEQGGEGVNM
                     SDIAVRTNFNETAFFFPQLRTNENGETVIEFTMPESLTKWKFMGLAHTKDLKMGSITK
                     NLVTQKTLMINSFAPRFLREGDKIIFTSKVSNLSETDMDGTVILELIDPLTGMSVADK
                     FGLTSNSMTFNVKKGQSTKIAWELSIPYGIGAVTYRIKAKSGAFTDGEEMSIPVLSNR
                     MLVTESLPLPIRGNETKNYTFDKLVNSGSSKTLVSHRYTLEFTSNPAWYAVQALPYII
                     EYPYECVEQTFSRYYANTIATFIANSYPKIREVFDTWKNLTPESFLSNLEKNEELKSL
                     VLEETPWVLDAQNESERKKRVGLLFDLNRMAKEQRKAENLLVKYQSPNGGFPWYKGMP
                     DDRYITQHIVSGFGKLDHLGIRDIKENSRISNMISKAVYYLDDRIREDLNYIKKHYPK
                     YKTEQHISYIQVHYLYARSFFLNYEISERNKEAFDYFFAQAKTYWTSFSTYGRGMIAL
                     GLNRYSEKTIPADIVKSLKETSLHSEEMGMYWREFAGGYYWWEAPIESQALMVEVFSE
                     VANDATAVEELKIWLLKQKQVQDWKSTKATVDAVYALLIRGFNLLTSDKIVEVKIGNE
                     VIDPFKIDNCKVEAGTGYFKTAWSGDQVKPDMGKIQVTKSDSGIAWGAVYWQYFEDLD
                     KITPHETPISIKKQLFVERQSPTGPRMEPIGDNATLKVGDKVKVRIEIRVDREMEYVH
                     MKDMRASCMEPVNVMSTTKYQSGLWYFENTRDAATNFFFNNLYKGTYVFEYSLFATHA
                     GEFSNGITTIQCMYAPEFTSHSEGIRVKVVE"
     gene            29589..30146
                     /locus_tag="PKY63_05080"
     CDS             29589..30146
                     /locus_tag="PKY63_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF00025.1"
                     /translation="MKEKNIISLSEKAREYAYWKHNRPSESQRYGSAPYSAHLENVVD
                     VIKKYIHLLDAECHEDVIAAGYLHDSVEDTDTTPEMLKSMFNLRIAELVLRVSNERGW
                     DKKEILFKTLPKIWQNKQATFLKLCDRIANGSNSRKGDSDRAKALYKRYSGEYPIFRY
                     ALKVEGEFNDMWNELDAIFDFEYPD"
     gene            complement(30155..30334)
                     /locus_tag="PKY63_05085"
     CDS             complement(30155..30334)
                     /locus_tag="PKY63_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPF00026.1"
                     /translation="MEEDIIICNCNEIYKSEIVKAIKEKGLKTVDEVGDATTAGTVCG
                     GCKDDIQEILDEINN"
     gene            complement(30413..32242)
                     /locus_tag="PKY63_05090"
     CDS             complement(30413..32242)
                     /locus_tag="PKY63_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00027.1"
                     /translation="MKKIFFILLIGFAATSLFAQDNEQKAIDELNLWSNDYNSALDYY
                     YEIKDKKEDSPEICNKAKEAYKLFNSAIQHCENAKNYFPEFSKDINLYLAETYSFMGW
                     FNLIKAPCVTNIFSDDVFNSCMKKAFEYRPFRSTVSLSDLKLDYYKQDKLNSLYGDQV
                     YLGYMNSGKQSNLGNRREYALEYINILKSEYGTDYINIITSDTSKTHQFIRLNNIVKV
                     SEEYLRDEELIESKIIMEELYAAWLANKKLSDPDAGFSQNDVDRLEKADKILAALKDD
                     GTSANTDQQLVRLYNANMIMDEKKIANDYLTEAKLHDHSSIDELYDYIEKTKAWRDET
                     SETDDRATAKDRIRRACNLIYEKMSTYSTSADYERLLAYYQYIDDSNGISTVQQKIAE
                     KKEEERKEQRREKLRSNWGLSVGIAPGKLIWQAKYNQLSLHADLVTAGFSHGFRYCKY
                     DLYTDKSRFGAYGVDDGEPGDNNTYSGWEGSWWINFKAVSGTKIFNFSPFLEFRYGQY
                     EFNSITTSVIDRETDMYAFTNVPVDPIGKRFDVTYGMKGTFTILNRFYFQTVFGFGLG
                     YRWLETGYDNEKYALECVNFSDERWPKVTVPFRIGFRAGIRLF"
BASE COUNT         9062 a         6305 c         7005 g         9941 t
ORIGIN      
        1 cctcaacctt aacctcaacc ttaacctcaa cctcaacctc aaacttagcc tcatgaaata
       61 cttcaccgca atttttctct taatctctct acattctctc gcacagaaac ctgcctacgt
      121 gctttcaaat ccttcaggaa aagaaatcgg atggactgca tttgttgatc agctttcagc
      181 agctgatatt gtctttatag gcgagtatca taacaatccg atctgccact ggatggagta
      241 tgaaatcacc gccgcgcttc acgaaaagaa ggccggaaaa attattttgg gtgccgaaat
      301 gtttgaatcc gacaaccagc ttttgattga tgaatatctg gcaggcaaaa tcaaacagaa
      361 aaacttcgag gacgaagcca aattgtggaa taattatcag accgactaca agccgctgat
      421 tgaatttgcc aaagaaaagg gattgctgtt tgttgccacc aatattccgc gccgatatgc
      481 gtcgatggtg gctgccggcg gttttgatgc acttgactca cttacagcag aggcaaaaaa
      541 acttattgca ccacttccaa tggcctacga tgaaaatctg aattgctaca aaagcatgat
      601 gagtatgggc ggcatgggcg gtggtcatgc caatgccaat ctgccaaaag cgcaggccgc
      661 caaagacgct accatggcgc atttcattct caaatatttt aagccggaat acacgtttat
      721 tcattataac gggtcttttc acagcgatta cagcgaaggc atagcctggt acatcaaaaa
      781 ggaaaatcct gctatgaaaa tcattgtcat ctccgtctct gaagtggatg atgttctgaa
      841 acccgctccc gaagacctga acaaaggcga ttacaccatc ctggttccgg ctacaatgac
      901 gaagacgtat tgagaaattt ccctacaaat tatttattct tccggcattg aaatttttca
      961 gtgccggatt tttttgtgat gtggaaagtt ttccatttat aatggatact tatgccgaaa
     1021 agtttccatt tgcgctggat actttattca tgatcgaaat aatgaattct ttcagaccaa
     1081 agtattattt ccaaagttat tacaactcca tcgaaagaaa atctctgagt tgcagatgct
     1141 gaattccatt aaatgtgtta ccggtgaatt ggtccattga aatgaccatt ttgggataat
     1201 tgtctttgat gcgttccaga tttccgaatt cgcggtctat ggtagcctga tcatgcagca
     1261 gataacatgt ttggatgtat agtttttcgc cttgcttttc tgcaacaaaa tctatctcat
     1321 aaggcccggt ttggcctacc ttgacttcaa aattattata tttcaaatgc ttgtaaacaa
     1381 cattttcgat tattttgcca atatcagttt gtttataatt ggaaatagta tttctgatgc
     1441 ctacatcttc aaagtagtat ttttctccta tttcaaatat tttcttcccg acaatgtcct
     1501 gccgttgtac tttgtttata aaaaaagaag atgtaagata atgaatatat ttaataatct
     1561 gcgctgttgt aatctgaatc cgctgtgact ttaaatattc gctgattttt cgtgccgaga
     1621 ataattgacc tgtattatca gccagaaatt caatcaggtt ttcaagaaaa gcactgtttc
     1681 tgatcttatt tcgcagaata acatcccgaa acataaccga gctgagaatt ccttttaaat
     1741 aatcatacac gatctcgtct ttcagctcaa gatgttttaa aaatggcatg cctccatact
     1801 tgagatattt tttcatcgag gcttcttggt tttttaatcc atgaaaaatc aaaaattccg
     1861 gataacttaa actataaacc ggaatttcaa tatatctgcc gcttaatagg gttgacaact
     1921 cgcctgatag catgttggcg ttactaccgg tacagtacag atcgtaggcc ggattcaaca
     1981 acaagcttct catacctttt tcaaaatctt ttatttcctg tatttcatca atgaatatgt
     2041 aatttctgat actttttgta ctgtgctgcg aaatgtattc aagcaaatct gtgctggttg
     2101 ctatactgtt gaactcaaca ttctctttgt tgatgtaaat gatttttccg cgtttatctt
     2161 tcttttctat ttgttgtatc gtctgtcgca taatatagct tttaccgact ctgcgctggc
     2221 cggtaaaaac ttttataatt ggtttttgaa taaaaggctt caccttattt aaatattttt
     2281 cgcgtatgat caattcagtt tccatgattt atgttcttta tgtgatacaa atcaatgcaa
     2341 atatacgaaa gtatattgta taaaatacaa aatatcgaat taaataaaag tgtattttgt
     2401 aaaagacaaa aatgtaccaa aaaacgcaca gataatcggg aaaaagtcga ttatctgtgt
     2461 aaaaagtcgt atatttgtgt tttattcctg ttatgaaaga actcagacag tttggctcgt
     2521 tcccgtttac tcacagcaat ttgttggaat tgctgcatgg atacaattcg ccaggagata
     2581 aaatatcgtc cctgataaaa aatggtgaca tcatctcact gaagcgaggt ttatacataa
     2641 acgctccgaa atatcgttcc tcgatggtct cgaaatttca gctggcaaat gttatttatg
     2701 gcccgtctta tgtgtcgatg gaatcggctc tatcagaata cgggatgatt ccggagggcg
     2761 tttttacaac cagatcggta acgctcaaaa gaagcagaaa ttttgtcaac tcattgggaa
     2821 agttcgaata ttttcatagt ccggaaaatt tttactccat tggaatcgga atcagtaacc
     2881 ggcaaaaaga aggatcgtgt ttgttggctt cacctgaaaa agcactttgc gataaaattg
     2941 ttttttcgaa aaattcgacc attcaatctg agaaagagat gctgaattat ctggaggatg
     3001 accttagaat tgacatgaat ttactaaagg atttggatga agcggttatt aatttgtgtg
     3061 ctgcagctgg aaggaagcaa gcacttttag agctggtgtt gaaaataatt tttaaattga
     3121 aaagatgagt ttattgaacg aactgatttt gcagcacaat ccatcagatg aaaatgagtg
     3181 gaaacaagcc atgcgtgaga gtatgcagat tattgcccta tctggtttgt atcggggaaa
     3241 tttttttgaa aatgcagctt tttacggagg tacatgttta agaatatttc atggtttgga
     3301 gcgattttca gaagacatgg atttttcatt gttacatgct gatgaaaact tttcgcttga
     3361 aaaatggttt gatcatttgc aacttgaatt caaagctgcc ggtacgacca ttgaaattga
     3421 gcgtaagagc aaaacacaaa aatcaatgat tgaatcagct tttttgaaat ctgacaccaa
     3481 agaatacgca ctccatttga aatcagagaa gcatctgaaa attaagattg aggttgacaa
     3541 aaatccacca ctcaggtttg agactgaaaa caaacttctt ttacaacctt tttcgttttt
     3601 tgttcagtca tttacattac catctctatt tgccggaaaa atgcatgcat tacttttcag
     3661 aagctggaaa aacagagtaa aaggaaggga ctggtacgac tttgaatggt acattagaaa
     3721 aggtgttgcg cttgatctgc aacatttcaa tgagcgttct gtacaaagtg gtcatttgca
     3781 aaattatata gacaggcaac aacttcataa actgctgata aataaaatta atgtccttaa
     3841 ttttaagtcg gctaaagatg atgtcgggcc ttttctaaaa tcatccgaca atcttgaaat
     3901 ctggtcaccg gaatatttta tagcattggt ggaaaaaatg aaaattgctt agaaatctct
     3961 tctgccttta aataagggtg gaatcagaaa aattgatttt ctttttctgt gctttctcag
     4021 aattttccgt aggtttgcgc aaataaattt gttcggatga gcttccgcga attattcaga
     4081 cgtaaatcgg ttgccgacat tgttgcgcag cacgaaaagg atgacaagcc catgaaacgc
     4141 acgctgggcg tggttgatct gactgcgctc ggtattgctg ccatcattgg tgccggcatt
     4201 ttcggaacca tcggcaaagc ggctgttgaa ggcggccctt cggtttcgtt actgtttgtt
     4261 ataactgcca ttgcgtgcgg attttcagcc atggcctatg cgcagtttgc cagctccatt
     4321 cccatcagcg gcagtgctta tacttacgct tatgccagtt ttggtgagtt gttcgcgtgg
     4381 atcatcggct gggacctgat tatggaatac gccatcggaa atattgctgt ggctatttcc
     4441 tggagcgatt attttacggg ctttctgaca ggttttggtt ggcatctgcc tgaattcatg
     4501 acggtggatt ttctcacagc atcaagagca tacgaaacgg taacacagct gttgtcggat
     4561 ggaactattt tcagtagtat tgatgcgcct ttgcgcgaag gatatctggc gtggaaaagt
     4621 gcgccacaga ttggtgatgt gcacctgatt gccgatatcc ctgcattttg tattgtggcg
     4681 ctgatttcaa ttcttgtttt tcgcggcatc cgtgaatcga aaattgccag taatttaatg
     4741 gtcggactga agctcattat tctgatggtg gtcattgctg ttggggcgtt ttatgtcaac
     4801 cccgacaact ggtctccatt tgcccccaac ggattttcag gcatgatgaa aggcgttgca
     4861 ggagtgtttt ttgcctacat cggattcgat gccatcagta ccactgccga agaatgtaaa
     4921 aatccaaagc gcgatctgcc tcgttccatg atgtatgcac tgggcattac cacagcaatt
     4981 tatatcatta ttgcgctcat tctgacagga atggtgcctt acgataaact cggagtaggt
     5041 gatccgttgg cttatgtgtt tgaacaggcc ggaatgccgc atctgagcgg gattatagca
     5101 ttgtctgcat taattgccat ggcgggtgta ctgcttgttt ttcaggtcgg acaacccaga
     5161 atatggatga gtatgagtcg cgacgggctg ctcccaaagc ggttttcgaa gttgcaccca
     5221 aaatataaaa cccctgcatt cagcacggtg attaccggta ttatggtggc ggttccggcg
     5281 ttgtttacca acctgactga aatgaccgat ctgacgagca tcgggacatt gtttgcgttt
     5341 gtgctggtaa cgggtggggt gattcttctg gataaaagtg atccggaaat cagaaaagga
     5401 ttcagaattc catatatgaa ttcgcgtgtt tgggttccaa tattgctggt ccctgtatat
     5461 attgcattat tcatgtttac cagcgatttt tcgctggaag cttttatggg tgaaaggatt
     5521 ccggttttaa tatattttgg tgtggccatt gttgttatga tagccgctgt aatccgccaa
     5581 tggagcttta ttccggtggt aggtctgttg attaatttat acctgatgtc agaacttggc
     5641 gtcaccaatt ggttgcgttt tttcatctgg ctggccattg gtctggtgat ttattttacg
     5701 tttggacgta gtcatagcaa actccgaaag cccgatggaa actagcgttg tcattattct
     5761 gatttgttcg ggactgattg ttggttttat caataccatg gccggcggtg ctactataat
     5821 ttccttatct gttctaacct ggcttggatt gccgcttaca gtggcaaatg gcaccaaccg
     5881 gctggccgtt ttttttcaga caatcacatc ggtgtcgaac tttcagaaca gtcatctgat
     5941 tgattggaaa aagagcattc gtgttggaat tccaaccgtc atcggatcag tgctgggttc
     6001 tttcattgcg gtgaagttga atgaagacat tatccggtat tcgtttgttg tgattatgat
     6061 tatcatgctt gcttttattc ttataaagcc atctttgtgg ctgaaaggca atgctgcgct
     6121 gcttgaactg ccggtgaaaa aatggaatta tccattattt tttatcattg gagtttatgg
     6181 tggctttttg catgttggcg tgggttatta tctgcttgcg actgttgtgc tggggcttgg
     6241 tctcgatctg atgagggcca acgtgatgaa aaatctgctg gtgatgttgt atgttccttt
     6301 ttcacttgtt ttgtttatcg tcaatgatga aatagcgtgg aaatacggtt tgattcatgc
     6361 aattggaaat gtaatcggtg cttttgttgc ttcgcggatt gccattaaaa gaggagcaga
     6421 tgttatccgt tggattatga ttgtagtaat agcgcttctg acagccgata tggcagggtt
     6481 gataaatatt aagtctgcta ttggtgtttt gcttgcaaaa tagattttga attgctattt
     6541 ttgctgaaat atctgatgta tgggaaaagg tggttttttg cggagatggc gcttgcaata
     6601 ctattatttt aaaagaactg gtttttatac cgaagtgggc aaaggtctga tcaattttat
     6661 tttaatattt gctgttgtag taggattggt gattattgcg gaaaaaacaa tattggatgt
     6721 cgatgctgtt tttaattacg ttgtaactca tgttccgaat tggctggttt ttcttgtggc
     6781 tttcagcacc gaactattat tcggattgat tccttccgat ttgtttatta tctggagcga
     6841 aggacatgat tatccctggg ttgcattgac cattcttgct ctgatgtcat atctggcagg
     6901 aatccttact ttttcatttg gcaaactcat tcgtaaaaat aaaaaggtca atagttattt
     6961 cacatcccgt ttcgaaaaac atattcgcaa cacaaggaaa tggggtggat ggctcgtagc
     7021 tgctgcggcc ctcacccctt tgccgtttac gctcatcatg gtagttgtag gtatgatcaa
     7081 ttatccaata aaacgtatga tgctgtggtc ttcattgcgc attccacgct attttctgat
     7141 ggcgctgatt tattatcata ccatcaatct gggtttgtag ggttgatgtg gattgatatt
     7201 acaacatggg attataacta ttgcttcaaa aaatatttgg tgttaaaagt ttattgctgt
     7261 ttcagaaaaa tgccgtaggt ttgtataaca actataaaca tgattctgaa aaaaatcaga
     7321 tatttttctc cgctgctgtt tttgttcatt tcagcattat tgcttttttt ggcgtattct
     7381 ttttcgggtg gtaaatccat caaaagtgaa aagcagatta acgcttttaa aacgtcgctt
     7441 gaaaaagccg ttactgaaac cgattctttg ctcaactggg cttcgaatcc aatgtttctg
     7501 aacgatccgt tttctatact ttccgtggga tcctttgttg ataaaaccag gtctttgaat
     7561 gcaaccgttt ttatcagtca caatgactct ctgatttttt ggagcgacaa ccgcacacct
     7621 ttgcctccgg acattgatac tctgcaatcg ggaaatctcg ttcagtcggg aaacggtgta
     7681 tatctgctgc gcgcttttca cgaaggcagt tatcggtttt tctctctgat gcttcttcaa
     7741 agtagttatt tttatcagaa caattatctt ataagtggcg tcaatccttg tttgtgtgat
     7801 gattgccgtt ttcagatttc ttctgatcaa accatggccg ataccttgat tcgtgttgac
     7861 ggtgtctctc ctttgatgcc gcttgtgttt tcaaaagatg acatttctct gtcttctgct
     7921 caggatgttg cagtggtttt attccttttt ttcgccctgg tttttcttgc catggcattc
     7981 tatggactgg cttcgcgttt ggccttgctg aaacatacta cgtggctccg acttgtttat
     8041 tttgcgttga ttccgctggt gttgtatttt tttggacagt atttgttttc attgaatttg
     8101 tttgatcagt taagtgtaaa caatcctgca ttttttgctt attccggccg gtttcccaat
     8161 ttgactccgc ttttgctgta tgcattttta tttgtgttgt ggatttcttt tttcagtcat
     8221 gaattacttt tgattgccgg aagaaaaaga aaaaatgttt ttcagcgtct cgcctttctt
     8281 ttcattacat acataacaac ggccacattg gcttattttg taatatcagg aatcaattct
     8341 tttttgttta atacatccat aaatttcact tttaacaatg ttctggctat agatattcgc
     8401 tctgtattgc tgcttcttac actctttttg cttttttgcg gcattattct tttgcattat
     8461 ctcatgatat cgttggtgag ccgtttgcgg ctttcattta aaacatggct cgttgtttct
     8521 gttgtttttc tgcttattgg agctctgact gctctgattg cgggtttcaa tgtgtggttg
     8581 tttgtttctg tttcggtata tctgctgctt atgcaatttg tcttttcgcg ccatatgtat
     8641 agcaacagaa cggtatatct gctgctcaat attgcattgg ctgcaggaat ttcaactgtt
     8701 tcgctcaatt atgccattca acagaaacaa tatgatcagc tgaaacttct ttcgcttaat
     8761 cttgctacag agcaggatcc cctaactgaa gaatttcttg gtgatttgat tccgcagatt
     8821 tctcaggaca gtattttact caatgatcct taccggatga ttgaagaatt taatctgaca
     8881 caatatctca aaagaaaata ttttaatggc tatctgggtc agtatattct gcagacaaca
     8941 tggtgcaggc ctggcgaaga aatacttgtt cagccttccg atacagcaat ctcctgcatc
     9001 ggcttttttg acgatgttgc ttttcgcatg ggtattcctg ctttatccga taatgttgtt
     9061 tttgtaaatg atggaaccgg caggggttat tatctgtgta aaataagact ggatgcgccg
     9121 ggcgatactt cttatatatt tgctgaactt attcctgctt cttacgaaaa aggacttggt
     9181 tatcccgatc ttttggttga tgtaaacagt ggaataaaaa ctgttccgtc aaatattgcc
     9241 tatgcgcgct atctcgatga tggtctggtt tcacgatatg gaaaattcaa ttatccgacg
     9301 cgtatcagcg ctctttttaa tacaatgcct gatgaaaaag tgattcatga tcatggctat
     9361 attcatttta aattgaccat ttcagatcgt cttgtattga ttattaccga aagcgatcgc
     9421 gatattcctt cttcgttggc tccttttagt tatattctgc ttttttttgg tgtacttgtt
     9481 gtcctttttc ttctgattgc tggtcgggtc tttggattca gacttcgttt tgccgtttcg
     9541 ttttcttcgc gcttgcaatg gatgattatt ttaattatcc ttgttatttt ctttgttgca
     9601 ggagtaatat cggtgcttaa tcttcaaagt ctgaataaaa gcaaaaacga agaaatagtg
     9661 agcgaaaaag cgcattctct cgtggttgaa ttggaacata agctgtcgca atatggacct
     9721 ttgtcttttg cagatgaaca gtatgttaca gagttgctcc ttaaattcag tcaggttttt
     9781 ttcaccgatc tgaatgtgta ttctctcaac ggaaagttgc tcgcaaccac ccgcccgcag
     9841 atattcgata aagaacttat ttctgtacgc atgaatgccg atgcttatgc aaagttgtct
     9901 caacgcaatc attcttttct gttgattaca gaacatatcg gtgaaatggc gtttatgtcg
     9961 gtttatattc ctgtccgaaa tgacatgaat gagaatattg cctatttaaa tctgccctat
    10021 tttgctcgcg aaaatgaact ttcacgcgaa atctcttctt ttatcagcac ttttatcaat
    10081 atttacatgc tgattatcct gctgactgtt cttataaccg tggtggtttc aaactatgtt
    10141 acacagccgt tgcgattaat aaaggataaa ctgaggaata tcaaactggg cacattgaat
    10201 gagaaaatta aatggaagcg tcgcgacgaa attggtgatc tggtttttga atacaaccga
    10261 atgattgagg aattggctta taaagccgat ttacttgcac ggtctgaacg tgaaatggcc
    10321 tggcgcgaaa tggccaaaca ggttgctcac gaaattaaaa atccgctcac acccatgaaa
    10381 ctcagcactc agtatctcga aaaagcatgg aatgataatg cagaagattt cgatgaacgg
    10441 ttgaaacgtt attcgcagat tatggttgaa caaatcgata gcttgagtga tattgcaacg
    10501 gcattttctc atttcggaaa aatgcctgaa tcgaccgtgg taaaaatttg cctgaatgat
    10561 gtgctgtcaa gtgttataac tttatatcgg agcgaggatt atgatataac gctgcaagtg
    10621 cccgatacgg ctttgtatgt ttttgccgat gaaagccaga tgctcagggt gtttaataat
    10681 ttgcttaaaa atgctcaaca ggcatttgtt cccgctcgtc gtggaatgat tcacgtaaca
    10741 ctgaaagttg aagaaaacaa ggcgctggtt gttgtgcagg acaatggtgc cggcatgtcc
    10801 gaagagcaaa agaaaaaaat atttcagccg aattttacca caaaatcatc tggaacaggg
    10861 ctgggattgg caatggttaa aaatattgtt tccggctttg gtggtcgaat ctggtttgag
    10921 tcggttgaaa atgtcggaac tacttttagt gttgagttgc cgttggcaga ataaaaaaca
    10981 tatcatagcc tgagatcgga aaatcaagaa ggaagcgacc cttagcacaa catgtttttt
    11041 gagctgatta tcaatatgtc agattccatc caatcttcga ttcccagttc ttttttatct
    11101 gcacggggcg gggtagtgta aatacttttt ctgcttgttt tttttgatcg aaaatacctg
    11161 gagtccattc tccatcttcg ttttcatcaa taatgatttt caaattgtat tctccgggca
    11221 gcatgttttt aaaaacgatt tgttcttttt cggccgatga aatcaatgta tatgtctttt
    11281 cttttgagct tgccgataca atcaagggct ttcccggcag attcatcagg tcgatttgca
    11341 atgatccgag gtcgctctcc tgaatcattc taaaagcgag ttttatgctg tcgtttttgt
    11401 atccaaaaat attagtggca gcaccttttt ttattataag cacagcgttt tcgccgggtt
    11461 tcagtttgtt aagtaaggtc cactgacggc ctgtttcatc tgatttctcc atcgacacgg
    11521 gcagggtatc tttgtttatt gcaagcaatg ttttagaaaa atcccaatgc tcaattggaa
    11581 tgatggatga aatactgatg ctttcgttcg gtgttatttt ttcacctttt gttgaagaaa
    11641 tccggaagag ggtgtcagtg gaaaaatctt tctggcgtcc tttgtatttc agtgcctgat
    11701 ataaagtgtc gctgaatgcg ccgtcgcgga tgatatatct gagcgaatcc gcatctggtc
    11761 ctttgatgta gaaaataaca gtgtcttttt tttcattcca ttcaaaggtg gtttctcctt
    11821 tcttatttaa aatggttatt tgcggatgtt caaaggacat gcgaaatgat gagcgcacag
    11881 tgtgataatg aatcatttct gttttcaggt gtttttgtgt gtttgctttt tctgtaaaca
    11941 gataaagatt gacggaatca attgttggtt tttctgctgt cagcaggtaa gggttgtttg
    12001 aaaatgcaat tttctcgtcc ggaatgtcaa atctgaaatt tgaattattg tcttccaatg
    12061 cacacaggta ataagaaccg gctgaaatat ttctgaattt gaatgcgcct ttggcatcga
    12121 catgtgcaat gtaaacaggc agtgtgtcgc gaaaatcatg gagtgattta tataacatga
    12181 cccaggcctt gtcggtaaat ttatttgtaa atgcatcgcc tgcaaatccg ctcagcgtgc
    12241 aactgtcgag ataagaaccg gtcgaaaaaa catagtccaa tcctgcagca gggttgtttt
    12301 cggtgatgtc ctgaatggcg ttgccaaaaa aaatatgata ggttgtattg ggcaataaag
    12361 aatcttttag ttcgattttt acttttttcc cctgaacact aaattccggt ttttcttttg
    12421 gaaagggatt gattataatc tgactgttag gattgttgag tttaataaac tcattaaatt
    12481 ccaatataat cagtttgttg tttgtatgaa tagatgagtc ggctggtgtt gccgaaaaaa
    12541 cttgaggtgg ggttctgtcg gccggacccc cgtttggctt ttcaaccgtg gcgcaggcgt
    12601 tgattaaaaa caacatgccg agtacataaa caaaacacaa caatttgttt tgttctgccc
    12661 gcacaattat attattaaag tagtctttca actttttgaa ctttaccaag gttttactta
    12721 ttattttttt caagcagttc aaacatcttc tgtttatacg cttctactcc tggttgatcg
    12781 aaaggattaa cgccttgaat ataggcgctt agtgcgcatg aaaactcaaa gaaatacata
    12841 atttcgccca gcgaaaactc agtcagattt tccatttcga gtgccaaaac gggaactccg
    12901 ccttgtgcat gggcaattct tgttgcttct tctgctttac ggtttacata gctcatttgc
    12961 ttcccttcaa gatagagcaa ttcatcgtca ttgctgttca atacaggtat ttcaatgtcc
    13021 ttttccggtt tggcaaccga taggaatgtc tcgaagagca cacggtttcc atcctgcaca
    13081 aattgtccca tcgagtgcag atccgtagta aaaaccagcg atgccgggaa aattccttta
    13141 agctctttac cttcgctttc gccaaaaagt tgtttccacc attcgctcaa ggttgacagt
    13201 ttggggcaga aagtgctttg cagttcaata tgtttgccct gtcggtataa tagttgacga
    13261 atggctgcgt aaagcatggc ctgattattt gccagcgtat ggtttgttgt taattctttt
    13321 tggcattcag cggcaccatt cagcagatcc tgtattgaga aaccgcagag agcaagtggt
    13381 aacagcccca ccggggtcag aaccgaaaaa cggcctccga tatcgtcggg gataatatag
    13441 gtttcatatc cctttgcaac agccagtgtg cgcagtgcgc cttttttctc gtcggtgacg
    13501 gcaatgattc tttgtgctgc atcagatccg tatttttctt ccatcagcgt ccttataagg
    13561 cggaaggcaa tggcgggttc ggtggtagtt cctgattttg aaatgacaac aattaccgga
    13621 ttacgttctt tgagataaac cataagcatg gcatgataag ccgagtccag ttgatgaccg
    13681 gcaaagatca tttccggctt tcgatggcct atagatggtg aaagagcatc gtaaacagct
    13741 ctggctccca gatagctgcc accaattcca atcacaacaa tcagatcggg gttgaatgtg
    13801 ctcacccgat ggcaggttgc ttcaattttg tttaggatat ctttgttttc cagtaatgaa
    13861 gacggcaagt ttacccagcc ggtaaattca gcgcctgagc cggttttgcc aagcagcatt
    13921 tctctggctt tctgaaccaa tggttcaata tccgaaaagt ctttctcaga tataaaattc
    13981 ttcactaaat ttacatccgc ttttgttttc atacatttgt gcttatgatg gtcaaaggta
    14041 caaactatat aaaaacaaaa atgcaggagt ggcgtcctgc attttgtgat aaaccaataa
    14101 ataaaccaaa aacctgaaaa tattttttac gtgtgtgcaa atcagatttt actctacaaa
    14161 gatatatcag tggcaaatgt gctcctgtta atgggtgtta agaagtgtta ataattgtta
    14221 atcgcgcaaa atgactaagg aaacgagact aaagacaatc attattgctg tatcagctgt
    14281 ggctttgatt ggcattgcct ctattcaata ttactacata cgaacagccg ttcatgcaag
    14341 cgaggttact tttgaccgta acgtgaacga tgtgatgtcg aagattattt ttcagatgga
    14401 gaaaaacgag gttgcttcgc aggttcgaag taaattgaag tctttttcgc gagaacagca
    14461 acttattaat acgctggatt cgttgaatca ggaattattc agatcgcttc agaaaattgg
    14521 tattgacagt atgagaactg atagcatcat tcgccaaacc cgcaatgaaa taagcgaaaa
    14581 ggttgctatg aatcagtatg gagaatatct gaaaaatgtc gatacgtcga aaattaaaaa
    14641 agatgtggta attcccaggg ccgattctct tttgtggatg gaattgcctt ttgccctgaa
    14701 agaaaagaag gcaagagccg atgctcataa aaatcttaaa accatacagg aaaaaagatt
    14761 ggatgagttg tatcgaaatg ttgacaggtt tttgcgccgc acatatctga ttggcgatgt
    14821 aatggaagat tttttcaaca tcaatcactt tttccctgtt gaaagccgta tcgattcggc
    14881 caaccttgat tcaatgatcc ggtccgaact ggctttcagg ggcattaccg ccgaagtcgt
    14941 ttttgggatt tacagcacca accgcgatac cattatttta caaagtgttc caggttttga
    15001 agaacggttg cggaattcca atttcggata taaactgttt cccagtgata tgttttcgtc
    15061 tccggattat ttaatgcttt attttccgca caaaagaaga tacatttatt ccgaagtttc
    15121 cggaatgctt atgcttagct tgtttcttgt gggtgtaatt gcggtctctt ttttctttat
    15181 tttgtttaat ttgctccgcc aaaaacggct ttcggaaatg aagacggatt tcatcaataa
    15241 tatgacgcat gaaatcaaga ctccgatctc tactatttcg ctggcttgtg aaatgcttgc
    15301 cgatcaatct gcatctattg atgaagaaca acgatccggt tttatacaaa ttattgcaga
    15361 tgaaaataaa agattggcag ccatggctga aaaaattctg caaacagcca ttatagacca
    15421 aggacgtatg gatctgaaaa aggaaaaaac tgatattcat cgtttgattg agaaatcagt
    15481 gaataatata aaactctggg ttaatcagaa agatggtttt gtggatgtga atctgagggc
    15541 tgctcatttt tttgccgatg tcgatccggt tcattttgaa aatgtgattt tcaatctgct
    15601 cgataatgcc attaaatatt ctccgtatca tccgcagatt tttattggta ccgaaaattc
    15661 aaatggacac attgttgtaa cggtgcgcga taatggcatt ggtattgaaa aaaaagaact
    15721 gaaaaaaata tttgacagct tgtacagagt gcctacagga aatattcata atgtgaaggg
    15781 ttttggattg gggctttgtt atgtcaaagc aattatttcc agtcatgaag gtacaatcaa
    15841 tgttgaaagc gaggtgggtc ggggaacagt ttttaccatt aaaattcctg tgttaaatga
    15901 aaaacaataa gatttttatt ttgttggctg aggatgatgt gaatatgggc ttgattctgc
    15961 agtcgttttt aacggctaag ggatttgagg ttttgttggc tcgcaacggt gttgaggctt
    16021 tgtctctttt tgggcggaat gcaggaattg atatggctct tgtcgatgtt atgatgcctg
    16081 aaaaagacgg gttttcgctg gctgctgatt tgaaagcaac ttcaccttcg ttgccgcttg
    16141 tctttcttac cgcaaaatcg atgcaggatg atattctgaa aggttttgaa atcggagccg
    16201 atgattatat taccaagcca tttagtatgg atgtgcttct ggcccgcatt acggctctga
    16261 taaaccgtac agtgaatatt cagaaggatt cggatcagcc acatcagctt ggaaaactct
    16321 ctttcgattt tagccgccag acaatttcgg atggtaaaga tgtggtgaaa atgacttcaa
    16381 aagaagctca attgcttaag ttgctggctg aaaacaagaa cagtattacc gatcgtaaac
    16441 tggcgctcga aatgatctgg ggtaacgatg actattacag ctcccgcagc atggatgtat
    16501 atattaccaa actccgtaaa atgctgaaaa tggataagtc catagagctt atcaatgttc
    16561 atgggcaggg ttataaactt atgtgcagct gattttgtat ttttgcacca atctattcga
    16621 aatgtctgaa aaaattcaac tttattctgc cagcgaattt atgaccattg ccaatgaata
    16681 ttgctggctg atggaaaata ttgctggttt tgaatccgaa aaagtgttgg attttatgcg
    16741 taaaattttg ccgttgctgt atgtaaaagg ttgtgctgtt gttgtgcccg aaggtgctga
    16801 tgaaacagat atgcagcgtt atgttactga agaaaattat gagattattt ttaatgaaac
    16861 acgtaataaa ctcaaagaat atgatgtttt ttttattttc aacaatgatt tgaaagaacc
    16921 ggaggagcgc agtattgcag aaggtctgac cgatatttat caggatatga aagatgtcat
    16981 gattgcatac aataagggat tggagccgga aaaggcagca gccctctatt gtctgaaaat
    17041 gtggttcatt gatcgatggg gtaagcacgc agctgacttg ttgccggttt ttcacaactt
    17101 atatgagaaa aaacattcaa atcaacaaac tggaacagag tttgattaat accgcgccat
    17161 gaaaaaaatt cttgtttttg tcgttttgtc actgacagtt ttgtcacttt ccctggctca
    17221 gggatataaa atttctgtaa aaataaatgg tctttctgaa gataccctgc tcatgggtta
    17281 ttattacgga aagtatcagt atgttcgcga taccattgtg cttgataaaa caggcaaagg
    17341 tgttttcgac accaaagata cggtaggtgg cggcatttat tttattgtct tgcctgacaa
    17401 aaattacatc gagtttgtac tggacaagga acgtgatttt tcaatcgaag ccgacacgtc
    17461 ggatcttgtc aaaagtgtac gggcaaaagg ttcgtcggat aatgcctcat ggtttgcata
    17521 taaggttttt attgccgaca aaggtgccga aatcgataaa ctcggcaaag tacataagcg
    17581 cctgcaggct actggtaagg aggattcggt aaaaattctc gaacagcaga tgaaagacat
    17641 caatgcggaa gttgccgact ataaaaataa atttattaaa gacaatccca agtctcttgt
    17701 atcaatcata tttgattgtt cgcgggaacc tgatattccc gaagcaccga tgctcccgaa
    17761 tggacgcaag gattcaactt ttgcctatcg ttattttcgt gcacatttct gggatcagta
    17821 tgattttacc gacgaccggc tgttgcggac acccatctat catgcaaaac tcgatcaatt
    17881 tctgaccaag gttgttcctc agattcctga tacgcttatt gccgaaatcg atgtcattat
    17941 cgaaaaaaca cgatccaaca aagaagtttt taaatatacg gtttggtatc ttacccatta
    18001 ttatgaaact tctcaggtta tgggaatgga tgctgttttt gtttttcttg ctgataaggt
    18061 ttatcgtgcc ggcgacgctt tttgggtttc ggacaatgtc aataagaaga ttattgaaca
    18121 tgccgataaa ttaaaaccca atcttattgg aaaaaccgca ccagaattgg ttttgctggg
    18181 gttaaacaat cagttgatct ccatgcattc catcccggct acctatacaa ttctttattt
    18241 ctgggaccct tcgtgcggcc attgtaaaac tgaaacgccc aaacttatta attatatcaa
    18301 taaggttaaa gacactttgg atatcaaggt ttattcggtt tgttcagata cgagtctgac
    18361 agcatggaga aattatgtgg cagaaaaaaa gatggaggta tttatcaatg taaacggaac
    18421 ccgcaccgcc aaaggaaatt tccatgacct gtatgatatt ttttccacnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ggaacccgca ccgccaaagg aaatttccat
    18601 gacctgtatg atattttttc cacaccggtc gtttatattc tcgatagtaa aaagcgtata
    18661 atcggcaaaa aaattccggt ggatcagatt ggaaattttc ttgcttttta tcgaaaacat
    18721 ccaacactga aagattaatc tgtgagtaat tctttttcca ggcgaatacg caatcagggc
    18781 atcgcatttg ccatgaccga cgtgctcgcc atgacgggtg tttatatatt ttcgaaaatc
    18841 tttctcaacg atttaccatt ccctgtcttt ggcgtttact ggttttcagc tgggttgata
    18901 tggaatctga ttctttcttt ttttgttccg ggtacatatt cgaaatttga aatcaaagga
    18961 tcatatcttg tttttctggt tgttgtcggg attcttgata tggcggctac tattctttgg
    19021 ttcgaagcgg ttgacaaaac cgacaatcct tcgctggtat ctttcatgac caacatcagt
    19081 ccggtttatg ctgttattct tggttatgtt tttctgaagg aaagactttc tctcagcgaa
    19141 ggaattggta ttattgttac tttggctggt gctgtgctta ttggttacag gaacgatttt
    19201 actttaaaca gctttctttt ttcaggagca ggaatcattc tgatttcatc acttatcagc
    19261 cagggtggaa aagtgattct gaaaacaaga ataagaaatt atcatcctat tgtattaagc
    19321 attaaccgga ttgtttttct tttgttgttt tcattttctt ttgtatttat ttttgattat
    19381 tcgctcaata taggttcaga aatgattgtt tatattgcca ttggagccct gatgggacca
    19441 gtgatttcag catttgccgg atacaatgcg ctggcgcggc tgaaggtttc gacctggtca
    19501 atattaagca ccagtcgctc attttttgtt atgcttgttt cctatttaat attgaataat
    19561 ttacccgata ccattcaaat cgccggcgga ttgctaacgg tagcaggtgt tgtagttatt
    19621 tctcttgcgc gtgtgaagtg atgtgtgcaa tgcacaaccc aacattctgt cgtccgccgg
    19681 aatcattttg tattggagcg gacacggaat cgttgggtgc cgcaaaaaaa cagaactcgc
    19741 aactcaccaa atgtgcttag cattgacctc tttgaatcat cccgccgaag cgggacaagt
    19801 tcccagcgaa gaaggaattt cgaattacga agtgaagttt gaatcgtgta gattactcta
    19861 atgaataatt ggtcaggttc actactcact gctcactact cactgctcac cgatcgccgt
    19921 tcactgctca ccgaaggtgc tcaaccttaa ccttaaccta tctgaagttg ctcaaaaaca
    19981 aagtcacaaa gcggtaatca agaatgatca taaaaatcac tccgtacaaa gcgccgaaaa
    20041 aatgcgcatc gtgaccgata ttgtcttttc cccggcggcc catataccat gaatagatca
    20101 ggtataggat tccgaaaata aaggcgggaa gctggattgg aataggaaaa atcatcaggc
    20161 ccgacatcgg atacatgaga atatacgcaa aggtgattcc ggaaacggct cctgatgcgc
    20221 ccaaggccga atactgcgga ttgttcagat ggcgtccgta ggaatacatg gatgagaaaa
    20281 tcaatccgcc gaaatacagc aacatgaaat acagtgtgcc ttcggagcca tagatgaatt
    20341 tcagcgattt ttctacaacc tcaccgaaaa tatacagtac ccacatgttg atgaaaaggt
    20401 gcatgaaatc ggcatgaatg aaaccatgcg taatgaagcg gtaccattgg ttttcgcgct
    20461 tgatctccca gggataaaaa agaagatggg caaacagcac acggttgctg aaagcaatga
    20521 gcgatactgc aacagtgaca ataataagta ttagtgtaat gctcattgtt taatgttgat
    20581 gatattgagg ttgtgtaaaa tcaatttcag atccgagaac gctgtgcgga ataagatata
    20641 cggctaattg cagaatgcat gcagccagca cccagccacg tttttgaggg tttttgcgga
    20701 tgataaaata tgcaacaacc catagaataa atgaaatggc tgttttatta tcagtcagat
    20761 cgtgaccgaa aggccagccg gtccagtatg catcaaaggc atatttctga acaatgggtc
    20821 caagaatgag cccgcctatc agcaatgtca gcatggacca gaatgtgaga cttaccagtt
    20881 gcttgcggcg tttaatggct tcaagtcctg ctcgtataca aaaaaccatt gacagaatca
    20941 tgaaaatcac atgcggaatc agcacccctg ccggcacttc gcctttgtag cggagaacaa
    21001 ctggatcttc tgtcagcgca acatgattgt tacgctcaaa aagaaaaacc tgatacatca
    21061 ttttaccggc tggcggcaat gcgggtaact gagcggaaag cagaccgttg tcatttttca
    21121 gcaccagcac tttccattca tcattgctcc ggaaacgccg gtattgaacc acccccgctg
    21181 catccggatg atttgttttt acttcgatgc gggcaccatc gctgccaccc caggtgcgca
    21241 gcattttgta tttaacggtt tcgcccagat attccacgct gccccgaacc ggagtcgtag
    21301 gccctgtcat acgctggtac accattaccg aaaccgccag tattattgcg agaatccaca
    21361 accacaaaga cttcatacac gctgttttcg gcaaaaatac taattttgtc aaattaccgt
    21421 ttctaagctg attaatgtca ttcatgaaaa tgctgcgatt tatttttccc gtactctccc
    21481 tgtgtgttct gctgctggct gccggctgcc gcaaggatga gcagttcaac gatgatcctt
    21541 cattcagact ggagttttcg gccgatacca tgtattttga tacggtgttc actacggtcg
    21601 gcagtgttac acttctggcc cgggttttta acaacagcaa tgactttgtc aatatctctt
    21661 caatttcact tggtggaggg accaatagta atttccgtat caatgtaaat ggtcttgaag
    21721 ggacttcgtt tactgatatt gaaatcggtc cgaaagacag catttttatc tttgctgaag
    21781 taaccgtcga tccgaccaat tctaacaatc cggtggtgat tgaggattcg atcaacttta
    21841 taatcaacgg aaatcatcag aaaattcaat tagtagcctg tggacaggat gcttgttttc
    21901 attttccgga tcatcctgaa acggagtatc tgcctgcgta ttcactggta tctggtgtgt
    21961 ggaacagcga caaacctcat gtggtctatg gttatgccgt ggttgatgaa gacagcgttc
    22021 tcaccatttc tgccggaaca caggtgtaca tgcacaacaa cgccgttcta tgggtctatg
    22081 atggcggcac gcttcaggtg tatggtgcga aagacaatga ggtgacattt cagggcgacc
    22141 gcctcgatga ctattacaag gacctgcccg gaatgtgggg caaaatctgg ctttcagccg
    22201 gaagcaagga taatatcatt gattatgcaa tcataaagaa cggccgcatc ggtgtgcatg
    22261 tcgatacgct cggagcgtcg gtcaatccta cgcttacaat gaccaacacc attatcgaca
    22321 acatgtcaac cgcgtgtttt gttgcgcagg gatcgtatgt gaaagcggaa aactgtgtgt
    22381 tcggaaacgc tggttatttc tcggttttgc tgaatattgg cggcaattac gatttccgtc
    22441 attgtaccat gggaaattac tggtcgcatg gaacccgcag tgcatcggcc attgtgctca
    22501 acaattatta cgaggacatt tacggcaaca cgcagatccg cgatctgacc aatgcttatt
    22561 ttggcaactg cattttgtat ggcgccaatg aagacgagct gctgctggac aaatatgcat
    22621 cgggcggagt tttcaatttt caatttgacc actgtttact caaaacaagt atgagcgtct
    22681 caggaagcgg atataccgat tgcatcagaa atcagaatcc tgcttttccg aatacccaat
    22741 tgcccgattt cagcattgtc agcggttccg ccgccatcga tgccggtgat gcagcggtga
    22801 ccggcaccat catgtacgat attcttggta atccgcgcat cgcaggcaca gctccggata
    22861 tgggggcgta tgagctgcaa tagcgctacg agtggaaagt ttataaagtt tttaaagttg
    22921 aacggcgacg taaatgcatg cggcaagctc caaacgtcac ctttataact ttacaacctt
    22981 acaactttca acccgcgaag cgaagtgccg cctgcctcga cctcgacctc gaccttgttc
    23041 tcgaccttga ccaccttgaa tattgaacag cgaacaagga atttcgaagg tagaagtgaa
    23101 gtttgaagaa ttgtatcctt caaaattctt acttccttgt tccttgttca gagttcctga
    23161 ccttaaattc atgctaaaac atatgcactc acagattatt tgtgaaataa cttttatctt
    23221 tactaattgt tgatgtgaaa aattttcttt tttgcatctg aaaaaagttt aataatgccc
    23281 aaaatatgaa aaacacattt ttgttcatga gtctgatcat tctcttttcg gcgcttccgg
    23341 ctttctcgca aaacgatgcg gcatatgaaa agctgtggaa aacaaccgac agtctgatcg
    23401 gaaaatcgct gccgcagtcg gcactggccg aagttgagaa gatttacgcc aaagccactg
    23461 cagagaaaaa caatgcgcag atcatcaagt cagtgattta ccgcatcacg cttatgtcgc
    23521 agtatcagga agattactac gagaattcga ttgccgaaat gaaaaaggaa atcgaaaagg
    23581 ccgagtttcc ggtgaagccc ctgatgtacg ctatgcttgg cgaaatgtac tggaattatt
    23641 acaccgtgaa ccgctataaa ttttatcagc ggacggccac cgataatttc gacaatgaag
    23701 atatcaaaac atggacgctg gatcgtcttg tcacagaagt gaaatggtgt tacgatcagg
    23761 cattgaaaga gccgcagaaa ctggctgatg taaaactgaa cacatatcag gcaatcattg
    23821 attattacga ttatctgcag tattcgccca ccatgctcga tcttatcggc tggatggcaa
    23881 tcacgcgttt ctcaaacgaa gaaaccgcac tcaccaagcc tgcacgcgtt tttaaaattg
    23941 acaacaagga ttattttttt ccgggacgaa gcttcgccgg aatgaaagtc gctgactatg
    24001 atacaacctc gtttgtgtat tatgcaacaa aggtttttca gacattgaca aagctgcatg
    24061 agaaagatgc tgattcggaa gtcttcactt ttgttgagct gcagcgactt gccttcatgc
    24121 gttcgaaatg cacgctgccc aacaaggaca cgctgtatct caatgcgctc aatgaacttc
    24181 atgcagccaa cgccggaaaa aaaggtcgtg ctgaaacggg atatgcgctg gctgtttatt
    24241 acaattcgct ggcctcgcga tatcattttg aaacagcccc tcagtataaa tggtataaca
    24301 aaacagcact tgaaataatt gaagaaacaa tacgtgatta tccgggaacg cgcggagcca
    24361 ataactgtaa tgtgctgaag gaaaccattc tcacccctgc aataacgctg aatttcgaaa
    24421 acacatcgta tcccggtaaa ccttttgaag gaatactttc ttattcaaat atcaacaatg
    24481 cctatttcag agtgattaag attgatcacg acaaagatcg cgagttggat aaaaaattga
    24541 gctacgaaga aattattgaa tattatgcga agcttactcc ggttaaggaa tttagtgtaa
    24601 tgctgactga tccgggcgat tatcagaatc acagcaccaa tgttgttttt gatggattga
    24661 gcgcagggta ttatattgtt cttcattcga gcaccaatga tttcagtatg gcaaaaggct
    24721 gggtcggtta tacatcgttg tgggcaactt ccatttcgta tctgatgcag gattacaaca
    24781 atcaggaaac cacatttctg gttaccgacc gcttgtcggg caagcctttg tcaggtgcac
    24841 aaatttccgt tttctacgaa aaatacaatt acattaccag ggattatgtg tataacaaat
    24901 acaaggtgct gaccaccgat gccagcggaa aagcttatct gggaacactt ccgaagggat
    24961 cggattatcg ttatatgtat gcggatgtgt cattcaagga agaccgttat tgtccttatt
    25021 cgacttttta catgtatccg ccttacgatt acagcaacaa tggtcaaact gtttcgtatt
    25081 tcttcaccga ccgcagtctg tatcgtcccg gccagacggt ttattttaaa ggcatcatgc
    25141 tgaaaaccga caaggacaac aattatagca tcctgccggg aacaaagtca acagttacat
    25201 tctacgatgc caattatcag gtggtgagcc aggttgaact gacttcgaat gattttggaa
    25261 ctttcactgg ttcatttaca gctcctgata atggaatcac aggaagcatg cgcattacca
    25321 atggctatgg cacggcctat atcagcgttg aagaatacaa acgtccgaaa ttttatgtgc
    25381 agttcgatcc ggtgaaggga tcgtacaaac tgaatgaaaa ggtctctgtg agcgggaagg
    25441 cgctggctta tgccggtaac aacatcgacg gtgccaaagt gaaataccgt gttgtacgca
    25501 atgcaagatt tccatactgg tggggctggt ggtcatacac catgccttac tcggcacagg
    25561 tcgaaatcac taatggagtt accacaactg atgaaaatgg agtgtttaaa attgatttca
    25621 cagcacttcc cgatgaatca gtcagaaagg attatctgcc ggttttcaat tatactattt
    25681 atgccgatgt gacagatatc aacggtgaaa cccgctccgg aaacacttgg gtgggagccg
    25741 gatacaaagc attaatactt ggttcaaatc tttcgcagga tattgtgaaa gggactgttg
    25801 aaaattatga aatcagttcg gaaaacttat ccggacagac tgttccttcg gatgtgacga
    25861 tgctgattta cacattgaaa tcgccggatc gtgtaatgag tcagcgtctt ctggcacagc
    25921 ccgaatacaa gtcgctgtca aaggaagagt atctgaaaat gtttcctttc ggcgtttttg
    25981 acaatgagaa caatccatca gaatgggaaa aaggaaaact tgtttattcg catttgtaca
    26041 atactgcaac tgattcgctg gtaaacatcg atgatgttct ggggggcatg cagggcgcgt
    26101 ttatgatggt tcttagtgca aaggattctt tcggagaaaa tgtcgaatat cagcgttttt
    26161 tcaattatta tgatccggct gaaaagaaat catgcagcaa tcaggtgttt tatgtacatg
    26221 aggttaatac aacctgtgaa cccggcgaga cagcatccat tcttgttgga agctttggtg
    26281 aggatgtgaa aattctggta gaagttgaac ataaaaataa aattgtcagc gcgcaatggt
    26341 acacgctgaa caaggaacaa aaactgttga cctttcctgt aaccgaggcc caccgcggaa
    26401 acttcgccat acatctggtc tgcgtgaaag gcaatcgttt ttattccggc aatgtgaatg
    26461 tgtatgttcc gtacaccgac aaacaacttg acattgaaat tgaatcgttt cgcgacaagc
    26521 tgcagccggg cgaaggtgag aaatggaaaa tcatcatcaa aaatgcaaag ggtgaaaaag
    26581 agctggcaga gctcatggca ggcatgtatg atgcttcact cgacgcatat aaaagtcata
    26641 gctggtatct gaatctcctg agttattact atggaaacag aagttgggac gcggcctcta
    26701 atttcacaac aaaatattct tatcagtatt cactggcgta tagtcgcaaa tattataatc
    26761 aaatgattta tacgccaaaa ctcagatggt tcaattaccc tctgtactct tacaatagtt
    26821 attattacag ctatgatgat gaagattatg gcgatattgg cgtattgtct tgttgtcaga
    26881 ctgctgcaac gggtaccaca cgcgaaaaag caagtgcaaa aaaagatgca cctgccgcta
    26941 tggatgtgct ggaagctgaa gaaatgctga tgtccgacgg agaagtggac aaagatgctg
    27001 aagttaccaa agccggtttg cttgcaggga atgatatgcg aaacgagcag gggggagaag
    27061 gcgtaaacat gagtgatatt gcggtacgca ccaatttcaa cgaaacggct tttttctttc
    27121 ctcagttgcg taccaacgaa aatggcgaga ctgtgattga attcactatg cctgaatcgc
    27181 tcaccaaatg gaagtttatg ggattggccc acacgaaaga tctgaaaatg ggatcgatta
    27241 ccaaaaatct ggtgactcag aaaacattga tgataaattc atttgcaccc cgcttcctgc
    27301 gcgaaggcga caaaattatt tttacttcta aagtcagcaa cctgagcgaa actgacatgg
    27361 atggcacagt aattctcgaa ctcatcgatc cgcttacagg catgtctgtt gctgataaat
    27421 tcggactcac ttcaaattcg atgaccttta atgtgaagaa ggggcaaagc acgaaaattg
    27481 catgggagct tagtattccc tatggtatcg gagcggtcac ctatcgcatc aaagcaaaat
    27541 cgggagcttt tactgatgga gaggaaatgt cgattccggt gctgtcaaac cgcatgctgg
    27601 tgaccgaatc actgcctttg ccaattcgtg gaaacgaaac aaaaaactat acgtttgaca
    27661 aactcgtcaa cagcggtagt tcaaaaacat tagtgagcca tcgctacaca cttgaattca
    27721 ccagcaatcc ggcatggtat gctgttcagg cattgcctta cattatagag tatccgtatg
    27781 aatgtgttga acagactttc agccgctatt acgcaaatac gattgcaaca ttcattgcca
    27841 atagctatcc aaaaatcagg gaagtgtttg acacctggaa aaatctgaca cctgaatctt
    27901 tcctgtcaaa ccttgaaaaa aatgaggagc tgaaatcgct tgtgctcgaa gaaacgccat
    27961 gggtgctcga tgcacagaat gaaagcgaaa gaaagaaaag agttggattg ttgtttgatc
    28021 tcaaccgcat ggcaaaggaa caacgcaaag cggaaaacct gcttgtaaag tatcagtcgc
    28081 caaacggtgg attcccatgg tacaagggca tgcccgacga tcgttatata acgcagcaca
    28141 ttgtatcggg atttggaaaa ctcgatcatc ttggaattcg tgacattaaa gaaaacagcc
    28201 gcatttcaaa tatgatttca aaagcggttt attatctcga tgatcgtatc cgggaagatt
    28261 tgaattacat aaaaaagcat tatccaaaat acaaaacaga gcagcacata agctacattc
    28321 aggtacatta tctctatgcc cgtagtttct ttttgaatta tgaaatcagc gaaagaaaca
    28381 aagaggcctt tgattatttc tttgcgcagg caaaaactta ctggacttca ttttcgacct
    28441 atggacgcgg aatgattgcg ctgggactga atcgttacag tgaaaaaacc ataccggccg
    28501 acatcgtgaa atcgctgaaa gagacttcgc tgcattcgga ggagatgggt atgtactggc
    28561 gcgaatttgc cggcggctat tactggtggg aagctcccat cgaatctcag gcgttgatgg
    28621 tagaggtttt cagcgaagtc gccaacgatg caacggctgt tgaagaactg aaaatatggt
    28681 tgctcaaaca aaaacaggtg caggattgga aatcgaccaa agccactgtt gatgcggttt
    28741 atgctttgct tatccgtgga tttaatttgt tgacttccga taaaattgtt gaagtaaaaa
    28801 ttggtaatga agtgattgat ccgttcaaga ttgacaattg taaagtcgaa gccggaaccg
    28861 gatatttcaa aaccgcatgg agcggcgatc aggtgaaacc ggacatgggt aaaattcagg
    28921 taaccaagag tgacagcgga attgcgtggg gtgctgtgta ctggcaatat ttcgaagacc
    28981 tggacaaaat tacgccgcac gaaacaccga tcagcatcaa aaagcaattg tttgttgaac
    29041 gccaaagtcc cacgggacca cgtatggagc ccattggcga caatgcaaca ctgaaagttg
    29101 gtgacaaagt caaggtgaga attgaaatcc gtgttgaccg tgaaatggag tatgtgcaca
    29161 tgaaagatat gcgtgcttcc tgcatggagc cagtgaatgt gatgagtaca accaagtatc
    29221 agagtggact ctggtacttc gaaaacacac gcgatgcggc caccaacttc ttcttcaaca
    29281 atctgtacaa aggaacctat gtgtttgaat acagcttgtt tgctacacat gcaggagaat
    29341 tcagcaatgg aatcaccacc attcaatgca tgtatgcgcc tgaattcaca agtcacagcg
    29401 aaggaatcag agtgaaggtg gttgaatagg aattccgttg ggtgtagtta aataaatatt
    29461 tactattggg cgtagtctcc agactacgcc cagcatatat ttataacaga ctatacccaa
    29521 caaataaaaa atattttact ttgtaaactg atttttgtct tatgtctgtg aagaaaaaag
    29581 tgtccgatat gaaagagaaa aacattattt ccctgtcgga aaaggccagg gaatatgcgt
    29641 actggaaaca caacagacct tccgaaagtc aacgttatgg ctcagctccg tattctgccc
    29701 atctggagaa tgttgtcgat gtgattaaaa aatacattca tctgctcgac gcagaatgtc
    29761 acgaagacgt tattgcagcg ggatatctgc atgattcagt tgaagacaca gacacaactc
    29821 cggagatgct gaaaagcatg ttcaacctcc gcattgcaga actggtgctg cgtgtatcga
    29881 acgaacgcgg ctgggataag aaagaaattt tgtttaaaac gttgccgaaa atctggcaga
    29941 acaagcaggc cacatttttg aaattatgcg accgcattgc caacggatcg aattcgagaa
    30001 aaggtgacag cgatcgcgca aaggcgctct acaagaggta tagcggcgag tatcccatat
    30061 tccgctatgc cttgaaagtc gaaggagaat tcaatgatat gtggaatgag cttgatgcta
    30121 tttttgactt cgaatatccg gattaatagc gctgctaatt gttgatttca tccagaattt
    30181 cctgaatgtc gtctttgcag cctccacaca cggttccggc agttgttgca tcgccaactt
    30241 catctacggt tttgaggcct ttttccttga ttgccttgac tatttcgctt ttgtaaattt
    30301 cgttgcagtt gcagatgatg atatcttctt ccattttttg ttgtaaagat aaagtttcat
    30361 ttgcgcaaaa gcattgcgaa atgacaaata tcagagtaat aaaatccggt tgtcagaaaa
    30421 gtctgatgcc tgcacggaaa ccgatgcgga acggcacggt tacttttggc cagcgctcgt
    30481 cgctgaaatt cacacattcc agcgcgtatt tttcattgtc ataaccggtt tcgagccagc
    30541 ggtaaccaag accaaagcca aagactgttt gaaaatagaa gcggtttaaa atcgtgaatg
    30601 tgcctttcat tccataggta acatcaaatc gttttccgat ggggtcaaca ggtacgttgg
    30661 tgaatgcata catgtctgtt tcgcggtcaa tgaccgatgt tgtgatgctg ttgaattcgt
    30721 attgtccgta gcggaattca aggaaaggtg aaaaattgaa gatttttgtg ccagaaacag
    30781 ccttgaaatt gatccaccat gatccttccc agccggaata ggtgttgttg tcgcctggtt
    30841 cgccgtcgtc aacaccataa gcgccaaagc ggcttttgtc agtatagaga tcatacttgc
    30901 aatagcggaa accatgactg aatccggcag taacaaggtc tgcatgaaga gaaagctgat
    30961 tgtattttgc ctgccagatt agtttcccgg gagcaatgcc gacgctcaaa ccccagttac
    31021 tgcgcagctt ttcacgtctt tgttcttttc tttcctcttc ttttttctct gcgatttttt
    31081 gttgcacagt gcttattcca ttgctgtcat caatgtactg atagtatgcg agcagacgct
    31141 cataatcagc agaagtactg tacgtactca tcttttcata aatcagattg catgctcttc
    31201 tgatgcggtc cttggctgtg gcgcgatcat cggtttccga tgtttcgtca cgccatgctt
    31261 tggttttttc aatataatca tacaactcat caatgctgga atggtcgtgc agtttggctt
    31321 cggtgaggta gtcatttgca attttctttt catccattat catgtttgcg ttgtaaagcc
    31381 gcaccagctg ttgatctgta tttgcagatg tcccatcatc tttcagtgcc gccagaattt
    31441 tgtcggcttt ttcaagccgg tcaacatcat tctgagaaaa ccctgcatcg ggatcgctca
    31501 gctttttatt ggccagccag gcggcataga gttcttccat gataattttg gactcaatca
    31561 attcttcgtc cctcaggtac tcttcggaaa ctttcacgat gttgttcaat ctgataaact
    31621 gatgcgtttt tgaggtgtcg gaggtgatga tattaatgta atcagttcca tattctgatt
    31681 tcaggatgtt gatatattca agtgcgtact cgcgtctatt gcccaggttg ctttgcttcc
    31741 cgctgttcat gtaaccaaga tatacctgat cgccataaag gctgtttaat ttgtcttgtt
    31801 tgtaataatc aagtttcaga tcgcttaatg aaactgtgct gcgaaatggt ctgtactcaa
    31861 atgctttttt catgcaggaa ttgaatacat catctgaaaa aatatttgtt acacatgggg
    31921 ctttgattaa attgaaccag cccatgaacg aatatgtttc ggcaagatag agattgatat
    31981 cttttgaaaa ttccggaaaa taattctttg cgttttcaca atgctgaatg gcagaattga
    32041 aaagtttata ggcttccttt gccttgttgc agatttcggg agaatcctcc ttcttgtctt
    32101 ttatttcata gtagtaatca agtgcgctgt tataatcatt gctccacagg ttcagctcat
    32161 caatggcttt ttgttcatta tcctgtgcaa aaagagatgt cgcagcaaaa ccaatcagca
    32221 gaataaagaa tatctttttc atatgaagaa tattgagtat ggtggcaaaa atacatattt
    32281 ttattttccc ccgaagggtg cttctcatcc ctcgaatggt ttggaaccct tcgaggaatt
    32341 aataaaaacc cttcgggaga ttattcagaa agccggacac ctaaacgaaa cgttgcaata
    32401 ttcaaaaaaa acc
//
LOCUS       DAOPKE010000026        32229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1466_length_32229_cov_11.783552, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000026 DAOPKE010000000
VERSION     DAOPKE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_32229_cov_11.783552"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..211)
                     /locus_tag="PKY63_05095"
     tRNA            complement(135..211)
                     /locus_tag="PKY63_05095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Arg,seq:acg)
     gene            complement(261..2798)
                     /locus_tag="PKY63_05100"
     CDS             complement(261..2798)
                     /locus_tag="PKY63_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00028.1"
                     /translation="MKKLIASFAVILLMTALQLSAQPWMKPPYLSKDRSEATFKDIQK
                     AFYSYWDDSPYVRGKGYKQFKRWEYLNEPHCFPDGKVKAAIKYMEVYEAAVAEAQQYR
                     QTTVAANWIPLGISSWQLGYSGYNPGNGRVNFVAQHPTNPDIIYIASPSGGIWKTTDG
                     GITWNTTYDQMTRLGTSCIAIHPLNPDTVFIGTGDKDSWDTEAFGIQKSHNGGLTWQN
                     GGLNTGINYNNINKILINPQNPQVMLAATPERIYRSVNGGANWTQVYNGNNDDIRCMV
                     YKPGDTTTVYCGGAMFLKSTNGGLSFTENTTLPTDTSRLEIGVSAANSNYVYVLASSD
                     DNTFGGLYRSTDNGDHFALMSTTPNILGYSVDSTAYTDDAGQAWYDLAIAVSPLDANT
                     IFTGGINIWKSTDGGANWAPITNWYVDPYYQYTHADIHYLGFYGDRFYCGSDGGVFVS
                     DDYGDTWTDLSPGLEITQFYAFANSQTNSQMIVAGAQDNGSNVFLNGTWTHAFGADGF
                     EALTYPGDDNTFYCSYQNGGILRTYDGGDNFDYINTTGQDGAWLTPIAMSPVNASVIW
                     MGLEDMWKSTDGGDNWINTTNGMLQGEKIDEMAIAPGNPLYVYFSNRDSLYISKNNGN
                     SWTAVQPASGYYITGIAVSEEDEEKIWISMSSTSGDRVYKSINAGDSWISITGTLSGV
                     GLNCIIEDPDSPDGLYVGTETGIFYRDTTMTAWEAFDANLPKVIVRELEIVSDVQRIR
                     AATYGRGIWESHMYTWNALKENKKFVCNIYPNPATKLVTIDLQNDANADLLRIFNAGG
                     SLIQTINIAGNKQSVDVSGLPTGNYYFRIEKNGEVLGTQKVTVVSEK"
     gene            complement(2812..3423)
                     /locus_tag="PKY63_05105"
     CDS             complement(2812..3423)
                     /locus_tag="PKY63_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPF00029.1"
                     /translation="MKIICIGRNYLDHVKEMKNEVPEYPVFFMKNENALIYNNLPFFI
                     PDFSNDIHYECELVLRICKTGKNIEERFASTYYNAITLGIDFTARDIQKRCIQKGEPW
                     EIAKSFDCSAPMGEFMPLTESGFPGDIEFRLEKNGAVVQNGNSKNLIFSFDHIISHVS
                     KFITLKTGDVIFTGTPAGVGPVAPEDLLEGFLAEKKVLTVRVK"
     gene            complement(4022..4843)
                     /locus_tag="PKY63_05110"
     CDS             complement(4022..4843)
                     /locus_tag="PKY63_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPF00030.1"
                     /translation="MKLKLNKSLAVFDIESTGVVVGVDKIIELFILRIDPDETEHEYY
                     ALLNPGMPIPPITTAIHGIKDEDVADKPFFKDIAHDLLKFLGNSDLAGYNSNKFDVPL
                     LIEEFLRCGIEFDYSKRRFLDVMGIFHKMEPRNLKAAYKFYCGKSIENAHTADADTRA
                     TWEILKAQLDRYENTEIEDEHGKLIKPIVNDVDALSKFSKTTRNADLVGHIIYNENGV
                     EVFAFGKYKWHPVEDVFQKEPQYYDWIMKSQFPESTKRLVNAIKLRGFNKGNVKA"
     gene            complement(4840..6573)
                     /locus_tag="PKY63_05115"
     CDS             complement(4840..6573)
                     /locus_tag="PKY63_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00031.1"
                     /translation="MKSVFFMLAVFALSTLLVHAQPGKEFVPEGNPKSADGYFKYQNF
                     KDALAEYLVLKAKDSLNVEYTHRIGLCYLYTNIDKTKAIPYLEWVVKQEKFDQNAWYD
                     LGRAYQYAYRFNDARTAFRKFASSGIKDNNPIPVERQLEICDQAEKLMKNPIDVSISN
                     LGPTINTPFPDYNPFIPKDESYLLFSSKRSGNMGGFIDYDGFLTADIYYSQRANAEWK
                     KAKQLSSTINTYLVEEVAGLTPDGELLFLYFDNEYGIADIFMSEGKGRTFKRPEGMGA
                     NINQKSFEGGATMTMNKKVLIFSSNRAGSIGGMDLWMSQELPTGEWGVPVNLGPGINT
                     QYDEDFPYIAPDGETMYFASTGHENMGGYDIFKCTYNKAGNTFSKPVNIGYPINTPDE
                     NISISFTASGRHAYISAVLPGGQGQQDIYRVTFNSVKPKKCFISGIIMTQDSTPLYEK
                     YKGMQIQLEEALAQLDSLKSMLGTEDTSSIEMTAPGLQAQIALLKKQTENLPQVVIRV
                     KDSKSGKLQGLYRPNKNTGKYIVIVEPGDYEFTYECPGYTTLVKQYVFHDDESSISNE
                     NEHVILIPETK"
     gene            complement(6624..8333)
                     /locus_tag="PKY63_05120"
     CDS             complement(6624..8333)
                     /locus_tag="PKY63_05120"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPF00032.1"
                     /translation="MNIEERINQWLNGSYDDETKNAIRRMQKENDPELQEAFYTDLDF
                     GTGGLRGIMGVGPNRMNKYTVGTATQGLANYLKESFRDTPVSVAIAYDCRNNSDYFAS
                     VAADVLSANNIKVFLFDALRPTPELSFTVRHLRCQAGIVITASHNPKEYNGYKVYWND
                     GGQLVPPHDKNVITAVRAIISIDQVKFERNSNLVTGIGKGIDEIYLRQIMQIVLSPEA
                     IEKQHRMPVVYTPLHGTGVTLIPKVLKRIGFGNIIHVPEQDVNDGNFPTVKSPNPEEK
                     SALEMAIAKAEETQAALIMATDPDADRVGVAVRNKEEKIVLLNGNQTAAILAYYTINR
                     LKETGKMPANPMMVKTIVTTDLLSAIAAESGVKMYDVLTGFKYIAEIIRLQEGKEKFI
                     GGGEESYGYLGADFVRDKDAVMTCCMIAEAAAWAACDGKTLCCLLDDIYSKYGYYAES
                     LLSITKKGMNGQQEIANMMKSFRENPPEEICGSTVKIIKDYKLRISKDMENSTETAID
                     LPISDVLQFFMKDGSKVTVRPSGTEPKIKFYFSVIMAQSGPQVTEAAHEKLSKLSAVF
                     NSN"
     gene            complement(8591..9451)
                     /gene="rfbD"
                     /locus_tag="PKY63_05125"
     CDS             complement(8591..9451)
                     /gene="rfbD"
                     /locus_tag="PKY63_05125"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPF00033.1"
                     /translation="MAKIWVTGANGQLGSELRELSKGCKDFEFVFTDMPELDLTKHDA
                     VKKAFKEIKPDVVINAAAYTAVDLAESEGEKTYAVNTYAVANLAALCSAGKAFLIHIS
                     TDFVFDGKRSCPYHEEVAANPVGTYAASKNKGEIEILLGTRHAAIIRTSWLYGNYGNN
                     FMKTILDKGRKGEKLSVVYDQVGTPTWSADLAKVVLEMARNAKKINGVELFHYSNEGV
                     ASWYDFAYEILSLSNSKSALVPVDSKDFQRPAPRPAYSVLNKDKIKKFLGIEIPYWKD
                     SLKRCLEQQK"
     gene            complement(9451..10386)
                     /locus_tag="PKY63_05130"
     CDS             complement(9451..10386)
                     /locus_tag="PKY63_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF00034.1"
                     /translation="MKKKVLVTGGAGFLGSHLCERLLNDGHEVLCMDNYFTGGKQNIA
                     HLLSNPYFELIRHDVTMPYFVEVDEIYNLACPASPIHYQYNPIKTIKTSVMGAINMLG
                     LAKRIRAKILQASTSEVYGDPDVHPQTEEYWGHVNPIGQRSCYDEGKRCAESLFVNYH
                     SQNNVRIKIIRIFNTYGPRMHPNDGRVVSNFIVQALKNEDVTIYGDGTQTRSFCYVDD
                     MVNGMIAMMNTGEEITGPINIGNPGEFTMLQLAEQVIRLTGSKSKIRHEPLPADDPMQ
                     RQPDISKAKEILHWEPKVNLEEGLIKTIEYFKNTI"
     gene            complement(10563..11120)
                     /locus_tag="PKY63_05135"
     CDS             complement(10563..11120)
                     /locus_tag="PKY63_05135"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HPF00035.1"
                     /translation="MKIRNNLAGNDLVEIPLSKRKLIWMTMGSLAFCCIGIWLLIDPH
                     KTDHPFLGSLAVTYMTGLVTFLFFGFGVFIFIRKLLSDNPGFIISRDGLTDNSSGVAA
                     GYIPWEDIQDVINAEIMNQRCILIMVKNPLEYLEKVRNPLKRKTMHMNYKMCGTPISV
                     SANSLKINYDNLFKLIIHKMDEYKS"
     gene            complement(11138..11260)
                     /locus_tag="PKY63_05140"
     CDS             complement(11138..11260)
                     /locus_tag="PKY63_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00036.1"
                     /translation="MMQRDMDKDNDFEYNDKEFYYIRLNLKTLELGGKIEIAMP"
     gene            complement(11296..11928)
                     /locus_tag="PKY63_05145"
     CDS             complement(11296..11928)
                     /locus_tag="PKY63_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPF00037.1"
                     /translation="MKNYYQILGLKESANTDEIKNAYRLYASKFHPDKHAGDKFFEER
                     FVEIKEAYDILSDKNKRLKYDLASGMSIDLKENSNWNNDNKPDETNIKHYDSIFVCIT
                     SEYIEDKTAKIKYLLSDIHEITFKEQFMKFAVLIGVLGVSLGIYLAEYQEDMTNAIIS
                     LFFGFICFFFKNRYLCITNKSGEMTKISITAGEENKIYDVLIRLRLKNMP"
     gene            complement(12521..14374)
                     /locus_tag="PKY63_05150"
     CDS             complement(12521..14374)
                     /locus_tag="PKY63_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00038.1"
                     /translation="MRTAIILFLLFPVLAIAQKEVRMPDYPSYQQVMTKFLTDYDILD
                     LKYPEEFRLTKNPDGWHAVLWNYESGDPSKDELFWSRKKNSFLSVSFKAASVKEITPD
                     EQAVIDDNMNIAYDAMSPYNFYRGWYKDVIHEFGKKKNLSDTMLNALARAYAHQAASL
                     LSDQYGLAVKSEVFSLSTGQNALSEEQLKTYRHWHNLSLETYKKLLAKNPAFETFIGD
                     ASNIYANEVMSGYLALMYFSNETEARKELRQGLYDDFMLKTARNYLNSCDQNAILFSN
                     GDNDTYPLLYVQAFEKFRTDVTVINISLLAVPRYISHLYENHSTQPVIRFSIARDYWN
                     NSSNDFYYIMNETDTMNAIDALKMTVGDDLANEDRGYALYPARHTILKWNDTDSISIF
                     PRENYIYLNHFAMLDIIASNVPERPVYFTITAQVPMIPAEYLQVEGLSYKVVPHITNN
                     PSNNFYSGGINPEQILIKIKNKFERPDLSKMAVAGNNHQMLSYSYRMVMYQAFIEFAI
                     KQDTLHALEMLDISEKWFPYSMFHANASAIPVVEYLYKLEMNSRADMISKEIINGIIK
                     EYPLESGSESDIQMAYSMLGYLENLLIEHCTELKLVQLINDKTELYRSKAK"
     gene            complement(14666..15988)
                     /locus_tag="PKY63_05155"
     CDS             complement(14666..15988)
                     /locus_tag="PKY63_05155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPF00039.1"
                     /translation="MKIAIVGSGYVGLVTGTCFAEVGIDTVCVDIDKEKIEKMKKGIS
                     PIYEPGLEPMMVRNIEKGKLTFTTDLASVVNNSDVIFSAVGTPPDEDGSADLQYVLDV
                     ARTIGQHMTKHVLIVTKSTVPVGTAKKVRQAIQDELDKRGLSIEFDVASNPEFLKEGD
                     AVNDFLKPDRIVVGVESKVAEDIMTRLYKPFTLNGHPVIFMDVPSAEMTKYTANAMLA
                     TRISFMNDIANLCELVGADVNMVRKGIGSDPRIGPKFLYSGTGYGGSCFPKDVKALIR
                     TSDEYGYSLRVLKAVEEVNDDQKSVLFNKLHVFFKGDLKGKKIAMWGLSFKPNTDDMR
                     EAPALVLVEKLLAAGCSVVAYDPVAMHEAQRKIGNIITYADTLYNALDGADALMLVTE
                     WTEFRYPDWNDVKKRLKTPLILDGRNIYDRKEMKENGFHYYCIGNKTE"
     gene            complement(16492..17355)
                     /locus_tag="PKY63_05160"
     CDS             complement(16492..17355)
                     /locus_tag="PKY63_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPF00040.1"
                     /translation="MGITLIADSGSTKTAWRGFAGKDIFLEVETVGLNPVYLSEESIS
                     SEVEKNLRPFIEFAKVERLYFYGAGCHSTEMQMKLQNALSLFFPESILHIHSDMEGAA
                     IGLFGNIPGIACIMGTGSNSCFWNGETIERNIPALGYVIGDEGSGAWMGKRIAADYLR
                     GVIPQDIAKILEKDYPTDLTFFLKQVYQNEFAGRFLASLTGKINHLQEHPYVIALETE
                     AIETFFRHHISLYEKKGAPLGFVGSIALNMKELLLRFCREKGYVVSSVVSNPLDGIQE
                     YLTDELYPEKR"
     gene            complement(17373..17876)
                     /locus_tag="PKY63_05165"
     CDS             complement(17373..17876)
                     /locus_tag="PKY63_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00041.1"
                     /translation="MKKIFFSLALLFITLNLAAQVKYNSPLGNFKATFPDAPEYKNSD
                     VDINNRTVKLHMFMHSETDNIFMVACADYPDGFFDTKEKREQYFISSADGFFGELNII
                     AGNAAKVKSGKYKGASYKGQGTDYGVLYHVYIAKNTIFQVVIMSNGAYPDEKAAKSFF
                     KSFRITI"
     gene            complement(17881..19575)
                     /locus_tag="PKY63_05170"
     CDS             complement(17881..19575)
                     /locus_tag="PKY63_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF00042.1"
                     /translation="MKLLLFFTALVLSAYSANSQTMGASYRVQFADKNNSPYSLSNPQ
                     EFLSQRAIDRRQSMNILIDESDIPVVQAYIDSLTHLGAVVLNRSRWFNTATVVLSDTT
                     IVSQIEALSFVSGVFKTKPYLTLKNKKPFINGTQSITHNIFNYPAAMMKATLELKRIS
                     HDYGLGLNQASMIGVDYLHGLGFQGENMVIAILDAGFYHVNTLSMFDSLRNQNRLLGF
                     KDFVLPGNNVFEESTHGMSVASLIVGNVPGSLVGTAPKASVWLLRSEDAGSEYIVEED
                     NWIAAAEFADSVGADVINSSLGYTEFDDPSQNHIYSDLDGNTTRISRGADMAASKGIL
                     VVNSAGNSGNNDWHFIGAPADADSILTVGAVNSAGTIASFSSYGPSADNQVKPTVCAQ
                     GQNAYVVSSSDGVYPSNGTSFSSPIMAGAVACLWQANKNKSNIDIIEHLKKSASHYYN
                     PDTMYGYGIPNMVVAHMILNGNNIDVQASQNKLMANPNPFGEEIDVVVYVTAAINANL
                     SIIDNNGKVVWTKDDMTFKSGFNYQLISGLGKLPAGVYILRINADNWNETVKLLKN"
     gene            complement(19583..20269)
                     /locus_tag="PKY63_05175"
     CDS             complement(19583..20269)
                     /locus_tag="PKY63_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00043.1"
                     /translation="MTSVRSCFILLIILLTGFSACKKDSILPPEDVGYSYFPYTPGSY
                     LIYNVDSTRYDDFYDTVMHFSFQLKEYYESYFIDAQGRPCIRIERWQKLCDTCNWTLR
                     DVWYSCLTDTYAERLEENVRITRMAFPVRKSTEWDGNAFNELDAQIFEYDEIDEPFVS
                     GFFSFDSTVTVIQDISYNLIEDNNQFEIYARHVGLVYKKFKDVEKDFVSGNIVSGVDY
                     TWQLTEYGHN"
     gene            complement(20279..21094)
                     /locus_tag="PKY63_05180"
     CDS             complement(20279..21094)
                     /locus_tag="PKY63_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00044.1"
                     /translation="MKRKSVFTSLLLIAFLSVFAQNPSDTLNQLDDNGRKHGYWKKYN
                     GDTLKYEGRFEHDVPVGKFIYYYVGGKVKSESVYSDNGKTTHTVMFFPEGSKLSEGIF
                     INQKREGLWKTYDGYDAVIAEVEYKNGQKWGLSKRYYQDGDILEVVNYINGWMDGVYV
                     QYFPDSVLKMKGTYANTKRNGIWAFYHGNGLIYMTGKYVDGLRQGDWIINDESGKIIV
                     REKYMNGKVVSREVFQKDKDPEELKKKDSQLDLENRGKTSNDNGIGDPLYDMY"
     gene            complement(21108..21704)
                     /locus_tag="PKY63_05185"
     CDS             complement(21108..21704)
                     /locus_tag="PKY63_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPF00045.1"
                     /translation="MDDPINKKQVLGQWKITPTWTLFLDRDGVVNQRKIGGYITVADE
                     FEFLPGVLQAIAVASKLFGKIIVVTNQQGIGKGLMTDEQLIQIHDHMLKRIAENNGHV
                     DKVYYSPFLAETNHHTRKPNPGMAMQAAKDFPQISFKRCIMLGDTASDMEFARSVGMK
                     AVFIGNPEEINIAGNSFDLDYPSFSDFIKDLNAVLVIQ"
     gene            complement(21715..22290)
                     /locus_tag="PKY63_05190"
     CDS             complement(21715..22290)
                     /locus_tag="PKY63_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPF00046.1"
                     /translation="MIEQRIRESIAVKQELLNDRKMIETIDRAVNAVIACFSNGHRLW
                     LCGNGGSAADAQHIAAELSGRFYLNRPPLPAEAFTVNTSYLTAVANDFSFNEIFSRLT
                     QAHCRPGDILVGISTSGNSANVLRALTLARDSGITTIALTGNNGGEMKHIADYSIIVP
                     SNDTPRIQETHILIGHIICELVESSLFRSLK"
     gene            complement(22283..23245)
                     /locus_tag="PKY63_05195"
     CDS             complement(22283..23245)
                     /locus_tag="PKY63_05195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF00047.1"
                     /translation="MNISFVIPLLNEEESLPELSNWIERVMKENNFSYEIVFVDDGST
                     DQSWKVINTLQASNPNIKAIRFSRNYGKSAALQSAFEQVEGDVVITMDADLQDSPDEV
                     PEMYRMIKEDGYDIVSGWKKKRYDNTFTKNIPSKFYNWTTRKMSGVKLHDMNCGLKAY
                     RIDVVKSIEVYGEMHRYLPVIAKWAGFSKIGEKVVQHRKRLYGKSKFGMSRFIRGPLD
                     LLTIMFVGKFSKRPMHFFGSIGSLLFFVGFVIAGYLAVLKIFYSVYKIADMPLFHLAL
                     LCMIIGSQLFIGGFLAEMIGRATPDRNKYIIREFLNLNKNKTDD"
     gene            complement(23246..23788)
                     /locus_tag="PKY63_05200"
     CDS             complement(23246..23788)
                     /locus_tag="PKY63_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPF00048.1"
                     /translation="MKEIIQKTVLKNGLYAGGILSAYFLLLYVSGLNYFSFSFITFLI
                     ETAAVLTFMFISISQVRKQTENNQIKTGTAMLIATVVMFIGLFCFQAMKLLVLFVIDP
                     AYNEMCVNEVFLQTLQVLKEYPQIAERIGNPEEIKNQLLIENNLLPFAIYLGKSVIVG
                     ILVGLVAKKKDKPEDSLISQ"
     gene            24002..24724
                     /locus_tag="PKY63_05205"
     CDS             24002..24724
                     /locus_tag="PKY63_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPF00049.1"
                     /translation="MIQNHGLLYNRLVADIHMQDGLSACINCGTCTAICPAAMFYRYD
                     PREIVNTVASRDEQKIEDLLKSDTIWACGECMSCKTRCPRGNAPGLIIIALRVLSEET
                     GLFTESEKGRQVLAIKRMIGESILDNGYCMWFDHINLEMFPEQGPTWQWVRDNASEVL
                     SKTGASYRKDSSGALRKIPQEDLDELKRIFDVTGGSKRYEIIEEFSRKKAEEMGLQFD
                     ETKECEYFKHIYSYNSYKLENK"
     gene            24721..25794
                     /locus_tag="PKY63_05210"
     CDS             24721..25794
                     /locus_tag="PKY63_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPF00050.1"
                     /translation="MKAEGKRHIWKEYQKEISGDRFWYARSCIRQNFFPGSETAFLRI
                     LQQELDKDVHDSACHTTCTGIGYHCDVVPFETTMTVVARQFALMTEKGYKNYAASCIT
                     SFGVYTEILETWESHPEVLERVRENLYKATGRTFEVPENLAHTSDIIFKFRNEIFEKK
                     RYALINAQTGKPLRGVEHIGCHYSKMFPHKGVGGAEYPQVLAGMIEDWGGEVVDYPER
                     RHCCGFGFRQYAVKANRGYSVANSHKKFESMEPYKPDFILTNCPGCPMFLDRWQYYIA
                     ETEGKTYGENGFGIPVLTYEELAGIILGYDPWELGLQMHQVSVEPLLDKLGYEYNKSA
                     KFKGMNGVCLSKPENPINLKICC"
     gene            25831..26871
                     /locus_tag="PKY63_05215"
     CDS             25831..26871
                     /locus_tag="PKY63_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF00051.1"
                     /translation="MSKKVVVIGGGPAGMESATYLANFGFDVSLVEKEDAIGGKLNAW
                     DRLFPSMRPGSEVKDFLEKGNQLSNVNVLLNTEIEKIDRNGSQMKLHTSKGEQIEADA
                     VVVATGFETFDATKKEEYGYGIYDNVITSKDLERTFREHKKIVRADGKPVKRVGIVHC
                     VGSRDEKAGNIYCSKVCCVTGVKQAIEINEAIPDSEVFCFYMDLRMFGMQFEELYKEA
                     QEKHGVQFIRGRLSEAAEDKDGSVIVKVEDTLSGRPLKMSVDLLILLVGFVPSPGTTK
                     MGKLFGIPFNSNRFLESDDEHTGTGMTKVPGVFMAGTCTAPRTITNSILDARSTAMRV
                     AGYLNDWKTEKR"
     gene            26991..27329
                     /locus_tag="PKY63_05220"
     CDS             26991..27329
                     /locus_tag="PKY63_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPF00052.1"
                     /translation="MSPEFGFKINKSAQIDLDKADDSLYRIICDLEPSLKVCIFCGSC
                     AGTCTAGQFTSFSFRRLSVELRRGMVEEVKEEISKCMLCGKCTLVCPRNVNTRHILYH
                     LKKHFDGNEL"
     gene            27313..29157
                     /locus_tag="PKY63_05225"
     CDS             27313..29157
                     /locus_tag="PKY63_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPF00053.1"
                     /translation="MEMNFDPFVLPFFLGLLVMGVILIWKYIVWMETIPVADRRVMFR
                     NMFTVRTFKAFKEAVMEGLFHRRIFKINPLLGYMHASLATGWFLLIVIGSIESKYGQK
                     DVFNMPWDPIFFNYFEPVKHGIEYGKFFVFMMDFLLLVVLMSVTFAVIKRFYSRLFGM
                     KKTTKLMIADQFALYSLWAIFPARLLAESLNAAHYSNGGFMTQPIGNLFGSFMSVNQI
                     INAEYTMWWVYSIVLAAFFVSMPFSRYMHILTEIFLIAFRHLGVKTADNLQGITRFEI
                     NACSRCGICIDKCQLSSAAGISNVQPAYFIQSVRYNLHFEDRAYNCLMCGRCNTYCPV
                     KIDVTNMRNVTRKLANETVSFRYDYLPEIKKDELAEVTNVVYFAGCMSHLTPSVPRAM
                     VALFKEAGITYNFVDENGSMCCGRPLMQSGLTEAAAELRAKLTNLINSRNSMILVTSC
                     PICYKTFKEDYNLKMHVVHHTELIKILIESKRLKPRMEDLSIVYHDPCELGRGCGEYE
                     NARIVLNQIGSIVSIDQEKEKALCCGGSISNLKVPYDKRELMAADAVKQLSAPNPDVI
                     VTACPLCKKTMAKYADGKMKDIAEVVAEAVGAVKQANKIEETEEVQVV"
     gene            complement(29176..29778)
                     /locus_tag="PKY63_05230"
     CDS             complement(29176..29778)
                     /locus_tag="PKY63_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00054.1"
                     /translation="MKAFLIPVFIFLFVSQSFAQSGYDVIAAEICDSISARCMDKDSA
                     TAMQMQQAYTLSAMLHHPEICDSLKKSIRMEIPGLDETSVMGIFSSRLAKNLIFNCES
                     FILITQYKTFASVPGKESLRLISSEMCGILENSDKTTLEDYQQLMNENLFPIVLRYQR
                     EIAADYSGGYESPEFIDDIAMYLLKHCLVLLKASLLNEYK"
     gene            29974..30654
                     /locus_tag="PKY63_05235"
     CDS             29974..30654
                     /locus_tag="PKY63_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPF00055.1"
                     /translation="MKKQCEDCAFRNEICRFIDEEEFSELNETTLYQHSPKGQTIVRQ
                     GDPMTHLLYLSSGLVKLEHVSPNKQIQIPALIKGPALISGNYQISGANNLLSVVAVEE
                     TNACLIDLVVLQSRMRKNAMFAMTIYDMLTAFHQRVLDKQLMLACKRVPGRIAGILLL
                     FREKFYNSNEFSFPLTRREISHLTSCSEENVIRTLSSFEQDGIITLSGKSVKILHPEK
                     LKRIYDIG"
     gene            complement(30755..31114)
                     /locus_tag="PKY63_05240"
     CDS             complement(30755..31114)
                     /locus_tag="PKY63_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPF00056.1"
                     /translation="MNIYISNLSYRLKDEDLKSVFEEYGEVTSAKIIMDRETGRSKGF
                     GFVEMPNDEAAAKAIKELHEATYDGKVISVNEARPREDRPKGNFGGGGNRGGYSGGGN
                     RGGGRGGYNSDSKPKRW"
     gene            31590..>32229
                     /gene="gpmA"
                     /locus_tag="PKY63_05245"
     CDS             31590..>32229
                     /gene="gpmA"
                     /locus_tag="PKY63_05245"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPF00057.1"
                     /translation="MKTLVLLRHGESEYNSQNRFTGWTDVNLSEKGITEARTAGKALK
                     ENGFEFDIAYTSVLRRAIKTLWLALEEMDRMWLPIKHSWRLNEKHYGTLQGLNKSEMA
                     AKYGEEQVLLWRRAYDVRPPLYDEADVKPFLNEARYKDVDNLIYMRGECLKDTVERVI
                     PLWENEIAPAIKSGKRIIIAAHGNSLRAVVKYLEGMSDEAILKYNIPTGIPLV"
BASE COUNT         8980 a         6798 c         6882 g         9569 t
ORIGIN      
        1 tcctttgggc tccgaattcc gagacaaatc cgcgcagctc ttgcagagct gaaataattt
       61 gccccaagtc actcttcgaa caagttcgat cgtgacccgg ggcaaattat ttttctcctc
      121 gcggatttgt ctcttggtga ccccgtcagg attcaaacct gaaaccttct gatccgtagt
      181 cagatactct attcagttga gctacggggc ctgattgaga gtgcaaaatt aataaatatt
      241 ttctttcgta caacaatttt ctatttttct gaaacaactg tgactttttg agtccctaaa
      301 acttctccgt ttttctctat gcgaaaatag taatttccag tcggaagacc gcttacatca
      361 acagattgct tattgccagc gatgtttatt gtttggatca gactgccgcc ggcattgaaa
      421 atccttaaca aatctgcatt tgcatcattt tgcaagtcta tggtcaccag cttcgttgcg
      481 ggattgggat aaatgttgca aacgaatttt ttattttctt ttaaagcatt ccatgtgtac
      541 atatggctct cccagattcc gcgcccgtag gtggctgcac ggattcgctg tacatcgctt
      601 acaatttcca gttctctgac aatgactttt ggcagatttg catcaaatgc ttcccatgcg
      661 gtcatggtgg tgtcgcgata aaaaataccg gtctcggtac cgacatacaa gccatcaggc
      721 gagtcaggat cttcaatgat gcaattgagt ccaaccccgc tcagggtgcc ggttatgcta
      781 atccatgagt ctccggcatt gatgctttta tacaccctgt cgccactggt tgaagacatt
      841 gaaatccaga ttttttcttc atcctcttca ctgacggcaa ttcctgtaat ataatatccc
      901 gaagcaggtt gaacggctgt ccagctgttt ccattgtttt ttgaaatgta cagtgaatct
      961 ctgtttgaaa aatatacata caatggattg ccaggtgcaa tggccatttc atcaattttt
     1021 tcgccttgca gcatcccgtt tgtggtattt atccagttat caccgccatc agtgcttttc
     1081 cacatatcct cgagacccat ccagattaca gaggcgttga cagggctcat cgcaatgggt
     1141 gtcagccagg cgccatcctg tccggttgtg ttgatgtaat cgaaattgtc gccaccatca
     1201 taagtgcgta atatgccacc gttctgatag gaacaataaa atgtgttgtc atcgccaggg
     1261 taggtgagtg cttcaaatcc atcggctcca aaagcgtgag tccacgttcc attcagaaaa
     1321 acattgctgc cattgtcctg agcgccggct acaatcatct gagaattggt ttgtgaattg
     1381 gcaaaagcat aaaactgtgt aatttcaagt cccggcgaca aatcggtcca ggtatcgcca
     1441 taatcatcgc taacaaacac accaccgtca ctgccgcagt agaagcggtc tccgtaaaaa
     1501 cccagatagt gaatgtcggc atgtgtgtat tgataataag gatcgacata ccagttggtg
     1561 attggagccc agttggcgcc accatccgta cttttccaga tattgatacc tccggtaaaa
     1621 atggtattgg catcgagtgg tgaaacagca atggccagat cgtaccaggc ctgaccggca
     1681 tcatcggtat aggccgttga atcgactgaa tatccaagaa tattgggggt tgttgacatc
     1741 agggcaaaat gatctccatt gtcggttgaa cggtacaagc ctccaaatgt gttatcgtcg
     1801 gacgaagcca gcacatatac ataattgctg tttgcagcgc ttacacctat ttcaaggcgg
     1861 cttgtatcag ttggcaaagt agtgttttcg gtgaaagaaa ggccgccatt ggtgcttttc
     1921 agaaacatgg cgccgccaca ataaacggtt gttgtatcac cgggtttata aaccatgcat
     1981 ctaatatcat cgttgttgcc gttgtacacc tgtgtccagt tggcgccgcc gttgaccgac
     2041 cggtaaatcc tttcaggtgt tgcagccagc atgacctgcg ggttttgtgg attaatcaga
     2101 attttattga tattgttgta attaataccg gtattcagtc cgccgttttg ccaggtcagt
     2161 ccgccattgt ggcttttttg tattccgaat gcttcggtat cccatgaatc cttgtcgccc
     2221 gtgccaataa atacggtatc gggattcagc gggtgaatgg ctatgcacga tgtgccaagc
     2281 cgagtcatct gatcataggt agtattccat gtgattccgc catccgttgt tttccagatg
     2341 ccgccactgg ggctggcaat gtaaatgata tccggattgg tcggatgctg cgcaacaaaa
     2401 tttacacggc cgttgccggg attgtacccg ctgtatccaa gctgccacga tgaaatccca
     2461 agcggaatcc agttggcagc aactgttgtc tggcggtatt gctgtgcttc ggccacagct
     2521 gcttcataga cttccatata tttgatggct gctttcactt ttccgtccgg aaaacaatgc
     2581 ggctcattca gatattccca gcgtttgaac tgtttgtagc ccttgccacg aacataagga
     2641 ctgtcgtccc aatacgaata aaatgctttt tgtatatcct tgaaggtggc ttccgatctg
     2701 tctttcgaaa gatacggagg tttcatccag ggctgggcag atagttgcaa tgcggtcatc
     2761 agcagaataa cggcaaagct tgcaatcagt tttttcatag tgatggtggt gttatttaac
     2821 gcgcacagtg agaacttttt tttcggccag aaatccttca agcaggtctt cgggcgctac
     2881 gggaccaacg cctgcgggcg ttccggtgaa aatcacatcg ccggttttga gtgtgatgaa
     2941 tttcgaaaca tgcgaaatga tatggtcaaa cgagaaaatc agatttttac tgttgccgtt
     3001 ttgcacaacg gctccgtttt tttcgagacg gaactcaata tcgcccggaa agccggattc
     3061 agtcagcggc atgaattcgc ccatgggggc ggagcagtcg aatgatttgg caatttccca
     3121 gggttcgcct ttctgaatgc agcgtttctg aatatcgcgg gcagtgaagt caatgcctaa
     3181 agttatggcg ttgtaataag ttgaagcaaa gcgttcttca atgtttttcc cggtcttgca
     3241 gatgcgcagc accagttcgc attcgtagtg aatgtcgttg ctgaaatccg gaataaaaaa
     3301 cggaagattg ttgtagatca gcgcattctc gttcttcatg aaaaacacgg gatattcagg
     3361 aacttcgttt ttcatttcct tcacgtgatc caggtagttt cggcctatgc agattatttt
     3421 cattgttttt tttcgttaca cgttacaaag tggaatttat cccgatgaaa gcgggaatcg
     3481 ttacgaattc tcagttcaat ctctctttcc acatttccat attttattca gggtgacaat
     3541 gtattgatgt acaaagataa ggaatgccgg tgagttttcg ttcagtgtgg cggttctgtg
     3601 atacaaatta tggtttgggt gtcaatcggg cttaggacat gccgtaatat ctttaacatc
     3661 tttccactac aattggcgcg catctcctgc ttgcaaccac gtaaaacagc ttgtcgcgtt
     3721 ttacgtgatg gcaagctgtt ttacgttgac gcgtgaccta acaactctaa ggtgcttcgc
     3781 acaccttgtt cctggggctt cgcccgaggc tattattatt taactcctcc ggagtatgag
     3841 gtggtgcttc gcaacttttt ttatgccttc ggctctatag gcgtaaggat ttaggtgaga
     3901 ggcaaggcat tgtgctttgc gccattgatc gcataccgca agccgcatgc ccctaacgtc
     3961 acctttataa actttaaaac tttacaactt tcaactcgcg aagcgccgca agccgcatgc
     4021 cctatgcctt cacattgcct ttattaaagc cccgcagctt gatggcattc accagtcgtt
     4081 tggtgctttc gggaaactgg ctcttcataa tccagtcgta gtattgtggt tctttctgaa
     4141 acacatcttc cacgggatgc cacttgtatt ttccaaaagc aaacacctcg acaccgtttt
     4201 cattgtaaat gatgtgtcct actaaatcgg cattgcgcgt ggtttttgag aatttactca
     4261 atgcatcaac atcgttcacg ataggtttaa tgagctttcc gtgctcatct tcgatttccg
     4321 tattttcgta gcggtcgagt tgcgctttca gaatctccca tgtagcacgg gtatcggcat
     4381 cggccgtatg ggcgttttcg atcgatttac cgcagtaaaa tttataggcg gctttgagat
     4441 tgcgtggttc cattttatga aagatgccca tcacatccag aaaacgacgt tttgaataat
     4501 cgaattcgat gccgcagcgc agaaattctt caatcagcag aggcacatca aacttattgc
     4561 tgttatagcc cgcaagatcg ctgttgccaa ggaatttcag aagatcatgt gcaatatcct
     4621 taaaaaatgg tttatcagcc acatcttcat cctttatacc gtgaatggct gttgtaatcg
     4681 gcggaatagg cattccggga ttcagcaacg catagtattc atgttctgtt tcatcagggt
     4741 caatgcgcag tatgaacaat tcaataatct tgtcaacacc aaccacaacg ccggtcgatt
     4801 caatatcaaa cacagccaat gatttgttca gttttaattt catttcgttt ccggtattaa
     4861 tatcacgtgt tcattttcat tgctgatgct gctttcgtcg tcgtgaaaaa catattgttt
     4921 tacaagtgtg gtatagcccg ggcactcata tgtaaattca taatcccctg gttcaacaat
     4981 tacaatgtat tttcctgtat ttttgtttgg acgatacaat ccctgcagtt ttcctgattt
     5041 tgaatccttt acacgaatca ccacctgagg aagattctcc gtttgctttt tcagcaatgc
     5101 aatttgtgcc tgcaatcccg gcgcagtcat ttcaatgctt gaagtgtctt cggttccgag
     5161 catacttttt aaagaatcaa gctgagcaag tgcttcttcg agttgaatct gcattccttt
     5221 gtatttttcg tacagaggag tgctgtcctg agtcatgata ataccactga taaaacattt
     5281 tttcggcttg acggaattga aggttacgcg atagatgtcc tgctgtccct gtccgccggg
     5341 taatacagct gaaatgtagg cgtgacgacc cgaagcagta aaagaaatag aaatattttc
     5401 gtcaggagta ttgattggat atcctatatt aaccggtttg gaaaacgtat ttcccgcttt
     5461 gttgtaggtg catttgaaaa tgtcatagcc acccatgttt tcatgtccgg ttgatgcaaa
     5521 atacatggtt tcaccatccg gagcaatata agggaaatcc tcatcgtatt gggtattgat
     5581 gcccggaccc agatttacgg gtacgcccca ttcaccggta ggcaactcct gagacatcca
     5641 taagtccatg ccgccaatag aaccagcgcg gttgctcgaa aaaatcaaaa cttttttatt
     5701 catggtcatg gtggcgccgc cctcaaatga tttttgattg atattggcgc ccatgccttc
     5761 gggacgttta aaggttctgc cttttccttc ggacataaaa atatcggcaa tgccgtattc
     5821 attgtcaaaa tacagaaaca gaagctctcc gtcgggtgtc agtcccgcaa cttcttcaac
     5881 gagataagta ttgatggtac ttgataattg ttttgctttt ttccattcgg cattggctcg
     5941 ttgagaataa tatatatcag ctgtgagaaa tccgtcgtag tcaataaaac cgcccatgtt
     6001 gccgcttcgt ttcgaagaaa acagcagata actttcatct ttcggaataa atggattgta
     6061 atccgggaaa ggcgtattga tcgttggtcc cagattggaa atactcacat caattgggtt
     6121 tttcatcagt ttttcggcct gatcacagat ttccaattgt cgttcaaccg gaatgggatt
     6181 gttatccttt attccactgg aagcaaattt tctgaatgca gtgcgggcat cattaaaacg
     6241 ataggcatat tgataggcac gaccaagatc ataccaggca ttctggtcaa atttctcctg
     6301 ttttacaacc cactcaagat aaggaatggc tttggtttta tcgatgttgg tgtacagata
     6361 gcacaacccg atccgatgcg tatattcaac attgagcgag tctttagctt tcagaaccag
     6421 atattctgca agtgcatcct tgaaattctg atatttaaaa tatccatcag ccgatttcgg
     6481 atttccttcc ggaacaaatt cttttcccgg ttgtgcatgc accaataggg ttgaaagtgc
     6541 aaacactgcc agcataaaaa agacagactt cattctcatg tcggttttta aaacaacgaa
     6601 tgtacaaaac ttcagcaagc caatcaattg ctgttgaaaa ctgctgacaa cttgctgagc
     6661 ttttcatgcg ctgcttctgt aacctgcggg ccactttgag ccatgattac gctgaaatag
     6721 aactttattt tcggctcggt gcccgatggc cgcacagtga ctttgctgcc atccttcatg
     6781 aaaaactgca atacatccga tattggcaga tctatagcag tttcagtgct attttccata
     6841 tctttcgata tgcgcagctt ataatctttt atgattttta ccgttgatcc gcagatttct
     6901 tccggcggat tttccctgaa cgatttcatc atattggcaa tttcctgctg accattcatg
     6961 cctttttttg ttattgacaa taagctttcg gcatagtagc catatttgct gtaaatatca
     7021 tcgagcagac agcagagcgt ttttccatca caggcagccc aggccgcagc ttcggcaatc
     7081 atgcagcagg tcatcacggc gtctttgtcg cgaacaaaat cggctccgag atagccgtag
     7141 ctttcttcac caccgcctat gaatttttct ttgccttcct gcaggcggat gatttctgct
     7201 atgtatttga agccggtcag tacatcatac atttttactc cggattcggc agcgatagcg
     7261 cttaataaat ctgttgtgac aatggttttc accatcatcg gattggccgg catttttcct
     7321 gtttctttca gtcggttgat agtataatat gcgagaatgg ctgcagtctg attgccattg
     7381 agtaaaacaa ttttttcttc tttgtttctg acagccacac caacacggtc ggcatcggga
     7441 tccgtggcca taatcagcgc cgcctgtgtt tcttccgctt tggcgatggc catttccagt
     7501 gccgattttt cctctggatt cggtgatttt acagtcggaa aattcccatc gttaacatcc
     7561 tgttccggca catgaatgat gttgccgaaa ccgattctct tcagcacttt cggaatcaat
     7621 gttacaccgg ttccatgcag cggggtatat acaacgggca tccggtgctg cttttcgatg
     7681 gcttcgggcg acaacacaat ttgcataatc tgtcgcagat aaatttcgtc aattcctttt
     7741 cctatgccgg tcaccagatt tgaattgcgt tcgaacttca cctgatctat gctgatgata
     7801 gcacgtacag cggtaattac atttttgtcg tgtgggggaa ccaattgtcc gccgtcgttc
     7861 caatacactt tgtatccgtt gtattctttc ggattatgcg aagcagtaat tacaatgccc
     7921 gcctgacagc gcagatgccg cacagtgaat gatagctccg gagtagggcg caaagcatca
     7981 aacaaaaaaa ccttgatgtt gtttgccgat aaaacatcgg cagccaccga tgcaaaatag
     8041 tcgctgttgt tgcggcaatc ataggcaatg gcaacagaaa ccggcgtatc gcgaaacgat
     8101 tctttcagat aattggccaa tccctgcgta gcagtaccaa ccgtgtattt attcattcgg
     8161 ttgggtccta cgcccatgat tccgcgcagg cctccggtgc caaaatccag atcggtataa
     8221 aatgcttcct gcaattccgg atcattttcc ttctgcatgc gacggattgc atttttggtc
     8281 tcatcatcat acgagccgtt cagccattgg tttattcttt cttcaatatt catatcgtta
     8341 cacgttacag tcgttacacg ttacaaagtt acagtcgtta cggattcttc gttaaatctc
     8401 tctttacaca ttttcacttc ggcgaagacg cacattttca cattcatttc gttacacgtt
     8461 acaattcagc tttcagttgt cagttatcag cgatcagctc tgagccgaag ctgagacgtt
     8521 acaattcttt gcaggatatc tctttccaca tttccgcatc ggcgaagccg cacatttaca
     8581 catcagtaca ttatttctgc tgttcgagac atctcttcag actatctttc caatacggga
     8641 tttcgatgcc caggaatttt ttgattttat ccttgttcag tacgctgtaa gccggacgtg
     8701 gtgcaggtct ctgaaaatct ttgctgtcaa ccggtaccag agcggatttt gaattggaca
     8761 gactcagaat ttcataagcg aagtcgtacc acgaggccac accttcgttg gaataatgga
     8821 aaagttcaac gccatttatt ttttttgcat tccgggccat ttcgagcacc actttggcca
     8881 gatcggccga ccatgttggc gttccgacct gatcatacac cacgctgagt ttttcccctt
     8941 ttcgaccttt gtcgagaatg gttttcatga aattatttcc atagtttcca taaagccacg
     9001 atgtgcggat aatggctgca tgacgtgttc ccagcagaat ttcaatttcg cctttgtttt
     9061 tgctggcggc ataggtgcct accggattgg ctgccacctc ttcgtgataa ggacaggagc
     9121 gctttccgtc gaagacaaag tcggttgaaa tatgaatcag aaaggctttt ccggccgagc
     9181 acaatgcagc cagattggca acagcataag tattgacagc ataagttttt tcgccttccg
     9241 attcagccag atcaactgca gtgtaagctg ccgcattgat gactacatcg ggtttgattt
     9301 ccttgaatgc ttttttgaca gcatcgtgtt tagtcagatc aagctctggc atatcagtaa
     9361 aaacgaactc aaagtccttg catcctttcg acaactcgcg aagctcactt ccaagctggc
     9421 cattggcgcc tgttacccaa atttttgcca tcaaattgta tttttaaaat attcaattgt
     9481 tttgatcaat ccttcttcca gattcacttt tggctcccag tgaagaattt cttttgcttt
     9541 cgaaatatcg ggctgacgct gcatcggatc gtcggcgggc agtggctcgt ggcggatttt
     9601 tgattttgat ccggttaagc gtatgacttg ttcggctaac tgaagcatag tgaattcacc
     9661 gggattgcca atgttgatcg ggcctgtaat ttcttctccg gtattcatca tggcaatcat
     9721 tccgttcacc atatcgtcaa catagcaaaa actacgggtt tgagtgccgt cgccgtaaat
     9781 tgtaacatct tcgtttttga gtgcctgaac gataaaattg gaaactaccc gtccatcgtt
     9841 tgggtgcatg cgcggaccgt aggtgttgaa aatgcggata attttgatgc gtacattatt
     9901 ttggctgtga taatttacaa acagcgattc agcgcatcgt ttgccttcgt cgtagcatga
     9961 acgctgtcct attggattta catgtcccca gtattcttcg gtctgcggat gcacatccgg
    10021 gtctccgtaa acctcactgg tgctggcctg caggatttta gcgcggatgc gtttggccaa
    10081 cccgagcatg ttgatggcgc ccatgaccga tgtttttatg gtcttgatgg ggttgtactg
    10141 ataatgaatg ggtgaggcag ggcaggccag attgtagatt tcgtccactt ctacaaaata
    10201 cggcatagtg acatcgtggc ggatgagctc gaaataggga ttcgacagca gatgcgcaat
    10261 gttttgcttg ccgccggtga aataattgtc catgcacaat acctcgtggc catcgttcaa
    10321 cagccgttca cacaagtgcg atccgagaaa tccggccccg ccggtaacca gtactttctt
    10381 tttcattcaa tgtatttgat ttgtaaaagt ataaaaattt tagcggaatg cggatatttg
    10441 gttaatggtt ataggttaat ggttactagt agtaagcagt ggtcagtttt atgaagttgt
    10501 aataatcata aacaagaaaa atatattaag aaatctattt gaagcggcca ataaattgtg
    10561 actcaggatt tatactcatc catcttatgt attatcaatt taaataagtt atcgtaattg
    10621 atttttaatg aattggcaga aacactaatg ggcgtgccac acattttgta attcatatgc
    10681 atggtttttc tttttaatgg atttcttact ttttcaagat attccagcgg gtttttcacc
    10741 ataatcagga tacaccgttg attcatgatt tcagcattta ttacatcctg aatatcttcc
    10801 catggaatat atccggcagc tacaccactc gaattgtcag ttagaccatc acgtgaaatt
    10861 ataaagcctg gattatctga taataattta cggataaaaa taaaaactcc gaatccaaag
    10921 aaaagaaacg ttacaagccc ggtcatataa gtgacagcca ggctccctaa aaatggatga
    10981 tcggttttgt gaggatcaat taaaagccat attccgatac agcaaaatgc aagtgagccc
    11041 attgtcatcc aaatcagttt tcttttgctc aacggaattt caacaagatc attgccggct
    11101 aaattgttgc gaattttcat ttattccgaa ttgagattta tggcattgca atttcaattt
    11161 ttccacctaa ttcaagtgtt ttcagattaa gtcggatgta ataaaattcc ttgtcgttgt
    11221 attcgaaatc attatctttg tccatatcgc gctgcatcat tatagttaaa tatcatattg
    11281 tattttatca tttgttcaag gcatattctt taatcgaagt ctaattaaaa catcgtaaat
    11341 cttattttcc tcaccagctg taattgaaat ttttgtcatt tcacctgatt tattagttat
    11401 acagaggtat ctatttttga aaaagaaaca gataaaacca aaaaataatg atataatagc
    11461 atttgtcatg tcttcctgat attctgccaa atatattcct aaactgacac ctaaaacgcc
    11521 tatcaataca gcaaatttca taaattgttc cttgaatgtt atctcatgga tatcagaaag
    11581 cagatactta attttcgctg ttttatcctc aatatattca gatgtaatac aaacaaaaat
    11641 actatcataa tgtttgatat tggtttcatc aggtttatta tcgttattcc agttggaatt
    11701 ttcctttaag tcaatactca ttccagaggc caaatcatat tttaatcttt tgtttttgtc
    11761 tgaaagaatg tcatatgctt ctttaatttc aacaaatcgc tcttcgaaaa atttatctcc
    11821 agcgtgttta tccggatgaa atttgctcgc gtataatcta taagcatttt ttatttcatc
    11881 cgtattcgcg gattctttta gtccgagtat ttgataatag tttttcaatt ttcaaactat
    11941 gaattcatta taataatgaa atgttttctt gtctccaaaa atacaaaaat tatagaactc
    12001 aatcaataaa actatatttc aaaacggcaa atcaaatccc tgtcacgacc cggagaattt
    12061 ttcgatttcg gatttgaatc aattaaaaac acaaccaccc gccttattgc tttttcttct
    12121 gtgcgaaaca gattcagcaa gaatcggcag gcagttgctg cccatgaaaa aaatgtaaaa
    12181 ccaatttacc ttacaatgac gagttgtttt aatttgtcga aattgattca gtcccgaaaa
    12241 ttattatcag aacgcacctg aatcaaagcc gttataagtt aacggaacca atcgttaagt
    12301 tttcggtaaa acatagtgaa atttgaatat gttactataa atattcccct gaagagttcc
    12361 aaacttttcg agggataaac aaaacccttc gaaaaatttt taccaaatga aattatttaa
    12421 cagaccctta ttctggttga ctccggagga gtcacatgtc tgtagaaaac aatatttata
    12481 aaacacgacc cgccagctgg cgggtcgaat gtttttcaag ctatttcgct ttactgcggt
    12541 acagctcggt tttatcattt atcagctgca ccagcttgag ttcagtgcaa tgctcaatca
    12601 ataaattctc cagatagcca agcatggaat aggccatctg aatatcggat tcagacccac
    12661 tttcaagagg atactctttt ataatgccat tgataatttc ctttgaaatc atatctgcac
    12721 gactattcat ttccagttta taaagatatt ctacaacagg aatggcgctg gcattggcgt
    12781 gaaacattga ataaggaaac cacttttcac tgatgtcaag catttccaat gcgtgaagcg
    12841 tatcctgttt tatggcaaat tcaataaagg cctgatacat aaccatgcgg tacgaataag
    12901 aaagcatctg gtggttattg cctgccacag ccatttttga aagatcgggt ctttcgaatt
    12961 tgtttttaat tttaatgaga atctgctccg gattgatgcc accggaatag aaattattgc
    13021 ttggattgtt tgtaatatga ggcaccactt tataactcag accttcaacc tgcagatact
    13081 ccgccggaat cattggtacc tgcgcggtga tggtaaaata caccggccgc tctggaacgt
    13141 tcgaagcaat gatatcaagc atggcaaagt ggttcagata aatgtaattt tctcgcggga
    13201 aaatactgat gctgtcagtg tcgttccatt tcagaatggt atgccgcgcc ggatacagag
    13261 cgtaaccacg gtcttcgttt gccagatcgt caccaacagt cattttcagt gcatcgatgg
    13321 cattcatggt gtctgtttca ttcattatat aataaaagtc attggaagaa ttgttccaat
    13381 agtcgcgggc aatgctgaat ctgatgactg gctgtgttga atggttttcg tacaaatggc
    13441 ttatatagcg cggaactgca agaagactta tattaatcac tgttacatcg gttctgaatt
    13501 tttcgaatgc ctgtacgtag agtaggggat aagtgtcgtt gtcgccgttc gaaaacagta
    13561 tggcattctg atcacacgaa ttcagataat tgcgcgccgt tttcagcatg aaatcatcgt
    13621 aaagtccctg tctgagctcc tttcgggctt ccgtttcatt tgagaaatac atcaaagcaa
    13681 gataacccga cattacttcg tttgcataga tgtttgatgc atcgccgata aatgtttcaa
    13741 atgcaggatt ttttgcgaga agttttttgt aagtttccaa agaaagatta tgccagtgtc
    13801 ggtatgtttt taattgttct tcgctcaaag cattttgacc ggttgataat gaaaagactt
    13861 cggattttac agcaagccca tactggtcgc tcagcagcga tgcggcctga tgcgcatagg
    13921 cacgtgccag cgcattgagc atggtgtcgg aaagattttt cttttttccg aattcatgaa
    13981 tcacatcttt ataccatccg cgatagaaat tgtatggcga catggcatcg tatgcgatgt
    14041 tcatattgtc atcgatcaca gcttgctcat cgggtgttat ttcttttact gatgctgctt
    14101 tgaacgatac cgataaaaag ctgttttttt tgcgcgacca gaacaattca tctttcgaag
    14161 ggtcgccgct ttcataattc cacagcacag catgccagcc atccgggttt tttgtgaggc
    14221 ggaattcctc aggatatttc agatccagaa tatcatagtc ggtcaggaat ttggtcatca
    14281 cctgctgata tgaggggtaa tcgggcatcc ggacttcttt ttgtgcgatg gccagcacag
    14341 gaaagagaag aaacaaaata atggctgttc gcattttgct tgggttttta caaatataca
    14401 aaattcagct ttcagaccga ggatgtttca atgcgacagc ttttcaaaaa caatattcca
    14461 ttgtccgaaa gcgacgaagg agctgaagaa tatggaatcg ttgtttttga atcatttacg
    14521 acgattccat attccggctg aaaacttcag ttattgcaaa acctgccgga acgatggaat
    14581 atcgtaacac ttcaaatatt gaaacatcct catgcgcaaa aaaaaagcgg agctttcgcc
    14641 ccgcctaatt gaaaccaatt tggaattatt cagttttatt gccaatgcaa taataatgaa
    14701 acccattttc cttcatttcc ttgcggtcgt aaatatttcg accatcgagt atcagcgggg
    14761 ttttcaggcg tttttttaca tcgttccaat ccggatagcg gaactcggtc cattctgtca
    14821 ccagcatcag tgcatcggca ccatccaatg cgttgtacaa agtatcggca taggtaatga
    14881 tgttgccgat tttccgctgt gcttcgtgca tggcaacggg atcgtaggcc accaccgaac
    14941 aacctgcagc cagtaatttt tctacgagca ccagagccgg cgcttcgcgc atgtcatcgg
    15001 tattgggttt gaatgaaagt ccccacatgg caattttttt acctttcaga tcgcctttaa
    15061 agaaaacatg aagtttgttg aacagcactg atttctgatc atcattaact tcttcaacgg
    15121 ctttgagtac gcgcagcgaa tagccatatt catccgacgt tctgataagc gcttttacat
    15181 ctttcggaaa acagcttccg ccatagccgg tacctgaata aagaaatttc ggtccaatgc
    15241 gcggatcgga accaatgcct ttgcgtacca tatttacatc ggcgccgaca agctcgcaca
    15301 gattggcgat atcgttcata aaactgatgc gggttgccag catggcattg gcagtgtatt
    15361 ttgtcatttc ggccgaaggc acatccatga aaatcactgg atgaccgttg agtgtaaatg
    15421 gcttgtagag ccgcgtcata atatcttcag ccactttcga ttcaacacca accacaatgc
    15481 ggtcgggctt cagaaaatca ttaacagcat cgccttcttt cagaaactcg ggattcgagg
    15541 ccacatcaaa ttcaatgctg agtccacgtt tgtcaagctc atcctgaatg gcctgacgga
    15601 cttttttggc agtacccacc ggaacagtac ttttggttac aatgagcaca tgtttggtca
    15661 tgtgttgtcc aatggttcgt gctacatcga gcacatattg caggtcagcg ctgccgtctt
    15721 cgtcgggtgg agttccaact gcagaaaaaa tcacatctga attgttcact acagaagcca
    15781 gatcggtggt aaaggtcagt ttgccttttt ctatattacg aaccatcatg ggttcaagac
    15841 ccggctcata aataggcgaa atgccttttt tcattttttc aattttctcc ttgtcaatat
    15901 caacacaaac cgtgtcgatg ccaacttcag caaaacaagt ccctgtaaca agaccaacgt
    15961 atccggatcc aacaattgct atcttcatgt atgtaaattt tagaatgcaa aaataacatt
    16021 aattcgcttt ctataaagga atcagttgtt gattgttatc atgaaggggg ttgcacaaag
    16081 tagaatcagg tcaaaggtca cgcgtcaacg taaaaccacg tgaaacagct tgtcgcgttt
    16141 tacgtgatgg caagctgctt caagcgggag acgcgcgccc agattaaggg gattgcacaa
    16201 agaaaggtcg aggccgaata gctgtcagat gtcagcgatc agcagtaggt gctgaccaaa
    16261 acgtcggtgc tctgagaaga agcttagtcg ttacaaacgt tacaatcgtt acacgttaca
    16321 aacgttacaa acgttacatg ttacaatcgt ggaatttatt cctttggaag cgaaaatcgt
    16381 gacaacgtga caggggttac aatggcaatt ttcaaatttt ctcatcagca aatcagcaca
    16441 tcaacatatt attttgtatt ttgtattcca catttcgcat ctcgtatttc atcaccgttt
    16501 ttccggatac aattcatcgg tcagatattc ctgaatgccg tcgagtggat tggaaacaac
    16561 agaagagacg acatatcctt tttcacggca gaatcggagc agcagttctt tcatgtttag
    16621 cgcaatggag ccgacaaaac cgagcggagc accttttttt tcgtacaaac tgatatgatg
    16681 acggaaaaaa gtttcaatgg cttcggtttc aagtgcaata acataaggat gttcctgcaa
    16741 atggtttatt tttccggtga gtgatgccag aaaacggccg gcaaactcgt tttgataaac
    16801 ctgtttcaga aaaaacgtca ggtcggttgg ataatctttt tcgagaattt ttgcaatatc
    16861 ctgtggaata acgccacgca gataatcagc agcaatacgt tttcccatcc aggcgccact
    16921 gccttcgtca ccaattacat atcccagagc cggtatattt ctttcaatgg tttcaccatt
    16981 ccagaagcaa gaattgctgc cggttcccat aatgcaggca atacccggaa tattcccaaa
    17041 caaaccaatg gcagcacctt ccatatcgct gtgaatatgc agaatcgatt cgggaaaaaa
    17101 cagactgagt gcgttttgca gtttcatctg catttcggtg gaatggcaac cggcaccata
    17161 aaaatacaat cgttcaactt tagcaaactc aatgaaaggt ctcagatttt tttcaacttc
    17221 ggagctaatg gattcttcgc tcagatatac cggattgagg ccgactgttt ccacttccaa
    17281 aaaaatatct ttgcctgcaa acccgcgcca ggccgtcttt gtagagccgc tatcggcaat
    17341 taatgtaata cccatcttgt tgttaagaat ttttaaatgg taattctgaa tgatttaaaa
    17401 aaagatttgg ccgctttttc gtcgggatat gcgccattgg acataattac aacctgaaaa
    17461 attgtattct ttgcgatata cacatggtat aatacaccat aatcagttcc ttgtccctta
    17521 taggacgcgc ccttgtattt tccgcttttc acttttgcag cattgccggc aatgatattc
    17581 agttcgccga aaaaaccatc cgctgacgag ataaaatact gttcacgttt ttcttttgta
    17641 tcaaaaaaac catcgggata atcggcacat gcaaccataa aaatattgtc ggtttcagaa
    17701 tgcatgaaca tatgcagttt tactgttcga ttgttgatgt caacgtcgga atttttatat
    17761 tcaggagcat caggaaaggt tgctttgaaa ttgcccaggg gcgaattata cttaacctgt
    17821 gctgcaagat taagggtgat aaaaagcaat gccagcgaaa aaaatatttt tttcatggtg
    17881 ttagtttttt agaagtttta ccgtttcatt ccagttgtca gcattgattc tgagtatata
    17941 aacgccagcc ggaagtttgc caagacctga aatcagctgg taattaaaac cacttttgaa
    18001 tgtcatgtca tcttttgtcc aaaccacttt accattattg tcaatgatag aaagattggc
    18061 gttgatggcg gctgtaacat aaacaacaac atctatttct tcgccgaaag gatttggatt
    18121 agccattagt ttattttgtg aggcctgcac atcgatgttg tttccgttta aaatcatatg
    18181 agctacaacc atattgggaa tgccatatcc atacattgta tccggattat aatagtggct
    18241 ggcgcttttt ttcaaatgtt caataatatc aatgttggat ttgtttttgt ttgcctgcca
    18301 caaacacgca acggcgcctg ccataatggg tgaactgaaa gatgttccat tgcttggata
    18361 aacaccatcg ctggacgaaa caacataggc attctgaccc tgggcgcaaa cagtgggttt
    18421 cacctgattg tcggccgacg ggccatagga tgaaaacgaa gcaatggttc cggctgaatt
    18481 aacggctccc acggtcaata tgctgtcggc atcggccggg gctccaataa agtgccagtc
    18541 gttatttcca ctgtttccgg cactgtttac caccagtatg cctttcgatg cagccatgtc
    18601 ggcaccacgt gatatgcgtg tagtgttgcc atcgagatct gaataaatat ggttttgcga
    18661 tggatcatcg aattcggtat atccaagcga agagttgatg acatcggcac caacactgtc
    18721 tgcaaattcg gctgccgcaa tccagttatc ttcttcaaca atgtattccg aaccggcatc
    18781 ttcggaacgc agcagccata cacttgcttt gggggcagtt cctacaagcg aaccggggac
    18841 attgcccaca atgagcgatg ccaccgacat tccatgagtc gattcctcga aaacattgtt
    18901 accgggaaga acaaaatcct taaacccgag caatctgttt tgatttcgca aactgtcgaa
    18961 catggagagg gtatttacat gataaaaacc ggcatcgagg atggcaataa ccatattttc
    19021 gccctgaaaa cccagtccat gcagataatc cacccctatc atactggcct gattgagccc
    19081 cagaccgtag tcatgtgaaa tccgttttaa ttcaagcgtt gctttcatca tggccgccgg
    19141 ataattaaaa atattatgtg tgatggactg agtgccgttt ataaaaggtt ttttgttttt
    19201 aagcgtaaga taaggtttag ttttaaaaac ccctgagacg aacgacaatg cttcgatttg
    19261 agatacgatg gttgtatcgg acaaaacaac agtggccgta ttaaaccagc gtgaacgatt
    19321 cagaacgaca gcccccagat gagtcagact atcgatatag gcctgtacaa cagggatatc
    19381 cgattcatca atgagaatat tcatgctttg acggcgatcg atggcccgtt gcgataaaaa
    19441 ttcctgcgga ttggacaggg aatacggcga attgttttta tcggcaaact gcaccctata
    19501 acttgcaccc atagtttgag agttggcact ataggccgaa agcacgagtg ccgtaaagaa
    19561 aagcaaaagt ttcatgtttt tatcagttat gtccgtattc ggttaattgc caggtatagt
    19621 ccaccccgga aacaatgttg ccggaaacaa aatctttttc gacatctttg aactttttat
    19681 agaccaatcc aacatgcctg gcataaattt cgaactggtt attatcttca atcaggttat
    19741 aagaaatgtc ctgaattaca gtaacagttg agtcaaaaga aaagaagccg gagacaaaag
    19801 gttcatcaat ctcatcgtat tcaaatatct gcgcatcaag ttcattgaat gcatttccat
    19861 cccattcggt gctttttctc accgggaaag ccattcgtgt tattcgtaca ttttcttcga
    19921 ggcgctcggc ataggtatcg gtcaggcatg aataccagac atcgcgcaag gtccagttgc
    19981 aggtatcgca cagtttctgc cagcgttcga tgcgtatgca tggacggcct tgtgcatcga
    20041 taaaatacga ttcataatat tctttcagct gaaaggaaaa atgcataacc gtatcataaa
    20101 aatcgtcgta gcgagtggaa tcgacattat aaatcaggta gctgcctgga gtatagggaa
    20161 aataagagta accaacgtct tcgggaggca aaatactatc ctttttgcag gccgaaaagc
    20221 cagtcagcaa aattatcaac agaataaaac agctacgtac ggatgtcata ttttcagatt
    20281 aatacatatc gtagagagga tcaccaatac cattatcatt ggatgttttc ccccggtttt
    20341 caagatcgag ctgagaatcc tttttcttta attcttcagg atctttgtct ttttgaaaca
    20401 cttcgcgcga aacaaccttt ccattcatat atttttcacg gacaatgatt ttccccgatt
    20461 cgtcattgat tatccaatcg ccctgacgaa gaccgtcaac atatttacca gtcatatata
    20521 tcaaaccgtt tccgtgataa aaagcccata ttccatttcg ttttgtattg gcgtatgttc
    20581 ctttcatttt gagaacgctg tcaggaaaat actgcacata aacgccatcc atccaaccat
    20641 tgatataatt gacaacttcg agaatatcgc catcctgata ataacgtttg ctcaggcccc
    20701 atttctgacc gtttttatat tcgacttcag caataactgc atcgtatcca tcgtaagttt
    20761 tccagagccc ttcgcgtttc tgattgataa aaattccttc cgaaagtttt gagccctcag
    20821 gaaagaacat gacggtatgg gttgtttttc cattgtcgga ataaacactt tcagatttaa
    20881 ccttgccgcc aacataataa tagataaatt taccaacagg aacgtcatgt tcaaaacgcc
    20941 cttcgtattt aagtgtatcg ccattgtatt ttttccagta accatgtttg cggccattgt
    21001 catccaactg attcaatgta tcggacggat tctgagcaaa gacactcaga aaggcgatta
    21061 acagcaatga agtaaaaaca gattttcttt tcatacagga aaacaattta ctgaataacg
    21121 agaacagcgt tcaaatcttt tataaaatcc gaaaaagaag gataatcgag atcaaaagaa
    21181 tttccggcaa tgtttatttc ttcgggattg ccgataaaaa cagctttcat accaaccgag
    21241 cgggcaaatt ccatatcgct ggctgtgtcg ccaagcataa tgcagcgttt aaaactgatt
    21301 tgaggaaagt ccttggcggc ctgcatagcc attcccgggt tgggtttgcg ggtatgatga
    21361 tttgtttcgg ccagaaaagg gctgtaataa actttgtcaa cgtggccgtt attttcagca
    21421 attcttttca gcatatgatc atgaatctgt atcagttgtt catcggtcat taatccttta
    21481 ccaattcctt gttgatttgt aacaacaatg attttgccaa atagttttga ggccacagct
    21541 atggcctgca aaacgccggg taaaaattca aattcatctg caactgtgat gtatccacca
    21601 atttttcttt ggttgacaac accatctctg tcgagaaaca atgtccaggt tggagtaatt
    21661 ttccattgtc ccagaacttg ttttttattg attggatcat ccatacagat aaaattattt
    21721 aagacttctg aaaagagaag actcgacaag ttcacaaatg atatgcccaa tcagaatatg
    21781 agtttcctga attcggggtg tatcgtttga aggaacaatg atagagtagt cggcaatgtg
    21841 tttcatttcg cctccattgt ttccggttaa agcaatagta gtgatgcctg aatctctggc
    21901 caaggtgaga gctctcagca cattggccga gtttcccgaa gtggagattc cgaccagaat
    21961 atctcctggc cggcaatggg cctgggtgag ccggctgaaa atttcattga agctgaagtc
    22021 atttgctact gcagtgagat aagatgtatt tacagtaaaa gcttcggcag gcaagggtgg
    22081 acggttcaga taaaaacgac cgctcagttc ggcggcaata tgctgcgcat cggctgcact
    22141 tccaccatta ccgcacagcc acagccgatg tccgttgctg aaacaggcaa taacagcatt
    22201 tacagctcgg tcgatggttt caatcatttt ccggtcgttg agcaattcct gtttgacagc
    22261 tatggattct ctgattcttt gttcaatcat ccgttttgtt tttgttgaga ttgagaaact
    22321 ccctgatgat gtatttgttt ctgtcggggg tagccctgcc aatcatttcg gccagaaaac
    22381 caccgataaa cagttgtgag ccaataatca tgcacagaag agccaggtga aacaatggca
    22441 tatcggcaat tttataaact gaataaaata tttttaaaac cgcgagatat ccggcaataa
    22501 caaagccgac aaaaaaaagt aaagagccaa tggagccaaa aaagtgcatc ggtcgtttgc
    22561 tgaattttcc aacaaacata atggtgagca aatccagtgg tccgcgaata aagcggctca
    22621 tcccaaattt acttttacca tataaacgtt tgcggtgctg cacaactttt tcaccaattt
    22681 tcgaaaaccc cgcccatttg gcaatgaccg gcagatagcg gtgcatttca ccatagactt
    22741 cgatgctttt taccacatcg atgcgataag cttttaaacc acagttcata tcgtggagct
    22801 tcacaccgct cattttgcgg gttgtccagt tataaaattt actgggaatg tttttggtaa
    22861 aagtattgtc gtatcgtttc tttttccaac ccgaaacaat gtcgtagcca tcttctttta
    22921 tcatgcggta catttccggg acttcgtcgg ggctatcctg caaatcggca tccatggtaa
    22981 tgacaacatc gccctccact tgttcgaacg ctgattgcaa cgcagccgac tttccatagt
    23041 tgcggctgaa acgaatggct ttgatgtttg gattgctggc ctgaagtgta ttaataactt
    23101 tccaggattg atcagtactg ccatcatcaa caaaaacaat ctcgtaacta aagttgtttt
    23161 ctttcataac ccgttcaatc cagttggata attcgggcag actttcttct tcgttgagaa
    23221 gtggaataac aaatgaaatg ttcatttatt gagaaattaa actgtcttcg ggtttatctt
    23281 tctttttggc aacgagacca accaaaatgc ccacaatgac agattttcca agataaatgg
    23341 caaagggcaa aaggttgttt tcgattaaaa gctgattttt tatttcttcg ggattcccga
    23401 ttctttctgc aatctgcgga tattctttca gcacctgaag agtctgtaag aaaacttcgt
    23461 ttacacacat ttcattgtag gccggatcta tcacaaaaag caccaacagt ttcatggcct
    23521 gaaaacaaaa gagaccaatg aacatcacaa ctgttgcaat caacatggct gtgcctgttt
    23581 taatctgatt attctcggtt tgtttgcgaa cctgcgaaat gcttataaac ataaaagtca
    23641 atactgccgc agtttcgata agaaaagtga taaaactgaa tgaaaagtaa ttcagaccgc
    23701 ttacatatag cagcagaaaa tatgcggata gaatacctcc tgcatacaat ccgtttttaa
    23761 gcactgtttt ttgaataatt tctttcatct tttttttgtt ggtagtgcaa aattaatcaa
    23821 ttatttcatt gcatttatat aagagtaaag aagctttttc tatttgcaga aatcgagtat
    23881 gtattttctg accgtaaaat tgccaacaat caggtaaaga attaccgatt gtcattcgtc
    23941 tttgattttg tcaggtaaat tatttttcat tactttgtcg actgaattaa tatacaaagg
    24001 tgtgatacaa aatcacggat tgctttataa ccggcttgtt gcagacattc atatgcagga
    24061 cgggctgagt gcctgtatca attgcggcac ctgcacagcc atttgtcctg cagccatgtt
    24121 ttacaggtat gatccgcggg aaatagtcaa cactgttgcc agccgtgatg aacagaaaat
    24181 tgaagattta ctgaaaagtg ataccatttg ggcctgtggc gaatgcatga gttgcaaaac
    24241 ccgttgtccg cgcggtaatg caccagggct catcattatt gcattgcgtg ttttatcgga
    24301 agagaccggg ctatttaccg aatctgaaaa agggcgtcag gtgctggcta taaagcgtat
    24361 gattggcgaa agcatactcg acaacggata ttgcatgtgg tttgaccata tcaatcttga
    24421 aatgtttccc gaacaaggtc caacctggca gtgggtgcgc gataatgctt cggaagtgct
    24481 gtcgaaaaca ggtgccagtt atcgcaaaga ttcttcaggt gctttgcgca aaattcccca
    24541 ggaagatctg gacgaattaa aaaggatttt tgatgtaacg ggaggttcaa aacgttacga
    24601 aatcattgaa gaattttcgc gcaaaaaagc cgaagaaatg gggcttcagt tcgatgaaac
    24661 aaaagaatgt gaatatttca aacacatata ttcctataat tcttataaac ttgagaacaa
    24721 atgaaggctg agggcaaacg acatatctgg aaagaatacc agaaagagat ttcgggcgac
    24781 agattctggt atgcccgaag ctgcattcgt cagaatttct ttcccggatc tgaaacagca
    24841 tttctacgaa tccttcagca ggaactagat aaagatgtgc atgattcggc atgccatact
    24901 acctgtaccg gaattggata tcattgtgat gttgtccctt tcgaaacgac catgacggtg
    24961 gtggcacgtc aatttgccct gatgacagag aaaggatata aaaactatgc tgccagttgc
    25021 atcacttcgt ttggtgttta taccgaaata cttgaaactt gggaatcgca tcccgaagta
    25081 ttggagcgtg ttcgtgaaaa tctttacaag gccacggggc gtacgtttga agtaccagag
    25141 aatcttgcac acacgagcga cattattttc aaattcagaa atgaaatttt tgaaaagaaa
    25201 agatatgcat tgataaatgc ccagacaggt aagcctttgc gaggagttga acatattgga
    25261 tgtcattact caaaaatgtt tccgcacaag ggagtcggag gtgctgaata cccgcaagta
    25321 cttgccggta tgatcgaaga ctggggaggg gaagtggttg actatcccga acgcagacat
    25381 tgctgtggtt ttggattcag gcaatatgcc gttaaagcca atcgtggtta ttccgttgcc
    25441 aactctcaca agaaatttga aagcatggag ccgtataaac ctgattttat tctgaccaac
    25501 tgtccgggct gtccaatgtt tctcgatcgc tggcaatatt atattgctga aacagaaggg
    25561 aaaacatatg gtgaaaacgg gtttggaata ccggttctga catatgagga actggctgga
    25621 attattctcg gttatgaccc ctgggaactt ggtttgcaga tgcatcaggt ttctgttgag
    25681 ccgttacttg acaagcttgg gtatgaatat aacaaaagtg caaagttcaa agggatgaat
    25741 ggagtttgtc tgagtaagcc tgaaaatccg ataaatctga aaatctgctg ttgatctgct
    25801 ttgcgaaaaa ataattgaaa aaaaaattga atgtcgaaaa aagttgttgt aatcggaggc
    25861 ggaccggcag gaatggaatc cgcaacttac ctcgccaatt ttggatttga tgtaagtctg
    25921 gttgaaaagg aagatgcaat tggcggaaag ctgaacgcat gggatcgttt gttcccaagt
    25981 atgcgtccgg gcagtgaagt gaaggacttt cttgaaaaag gaaatcagtt atccaacgtc
    26041 aatgttttgt tgaatacaga gattgaaaaa attgaccgca acggatcaca aatgaaactt
    26101 cacaccagca agggtgaaca gattgaagcc gatgcagtgg ttgttgctac cggatttgaa
    26161 acattcgatg caacaaaaaa agaggaatat ggctacggta tttacgacaa tgtgattacg
    26221 agcaaagatc tggagcggac tttcagagag cataaaaaaa ttgtgagggc cgatggcaag
    26281 ccagtgaaac gagttggtat agtgcattgc gttggttcgc gcgacgaaaa ggcaggcaat
    26341 atttattgtt cgaaagtgtg ttgtgtaacc ggtgtcaaac aggcaattga gattaatgaa
    26401 gctattccgg attcagaagt attttgtttc tacatggatt tgcgaatgtt cggaatgcaa
    26461 tttgaagaat tgtataagga agcacaggaa aaacatggcg tacagtttat ccgcggacgc
    26521 ttgtcggaag cagccgaaga taaggatgga agtgtgattg tgaaagttga agatacactc
    26581 agcggacgcc cgttgaaaat gtcggttgat cttttaattc tgctggttgg atttgttccg
    26641 tcgcccggca caaccaaaat gggaaagctg tttggcattc cattcaattc aaatcggttt
    26701 ctcgaaagcg atgatgaaca cacaggaaca gggatgacaa aagttcccgg tgtatttatg
    26761 gccggaacct gcaccgcacc gcgcaccatt accaacagca ttcttgatgc acgcagcacc
    26821 gccatgcgtg tggccggata tctgaacgac tggaaaacgg aaaaaagata gattcagcag
    26881 tgagtggtga atagtgagtt agaaagaata aattaatgtg tctttataaa ctttatgaac
    26941 tttaaaaact ttataaactc aactgaaaac agaacgaaca caaacaaaca atgagtcccg
    27001 aattcggatt taaaataaac aaatcagcgc agatcgatct cgataaagcc gatgattcat
    27061 tgtatcgcat tatttgtgat ctggagccca gtttgaaggt gtgcatattt tgcggttcat
    27121 gtgcaggaac ctgtacggcc ggtcaattca catcgttcag cttccgtcgt ttgtctgttg
    27181 agttgcgcag gggaatggtt gaagaagtga aagaagaaat atcgaaatgc atgctgtgtg
    27241 gtaaatgcac attggtttgt ccgcgcaatg taaacacgcg ccatattttg tatcacttaa
    27301 aaaagcactt cgatggaaat gaactttgat ccttttgtgc tgccgttttt cctcggtctg
    27361 ctggtgatgg gagtcattct catctggaaa tacattgtat ggatggaaac gattcctgtg
    27421 gccgatcgcc gggtgatgtt ccgcaacatg tttacagtgc ggactttcaa agcattcaag
    27481 gaagccgtga tggaaggatt gtttcaccgc cgcattttca agatcaatcc tttgcttggg
    27541 tacatgcacg ccagtcttgc aaccggttgg tttctgctca ttgtgatagg atccatcgag
    27601 tctaaatatg gacagaaaga tgtgttcaac atgccatggg atcctatttt ctttaactat
    27661 tttgaaccag tcaaacatgg aattgagtat ggaaaattct ttgttttcat gatggatttt
    27721 ctgttgcttg ttgttttaat gtctgtaaca ttcgctgtaa tcaagcgatt ttactccaga
    27781 cttttcggca tgaaaaaaac cactaagctg atgattgccg atcagtttgc actgtattcg
    27841 ctttgggcca ttttcccagc ccgattgctg gctgaatcac tcaatgctgc gcattacagc
    27901 aacggtggat ttatgaccca acccatcgga aatctctttg ggtccttcat gtcggtgaat
    27961 cagattatca atgcggaata cacaatgtgg tgggtatatt caattgtgtt agccgctttc
    28021 tttgtatcca tgccgttttc gcgctacatg catattctga cagaaatttt tctcatagcg
    28081 tttaggcatc tgggtgtaaa aacggctgat aatctgcaag ggattacacg gtttgaaatc
    28141 aatgcatgca gccgttgtgg tatttgcatc gataagtgcc agctgagttc tgctgctgga
    28201 atcagcaatg tgcagccagc ctattttatt caaagcgtac gatataatct gcatttcgaa
    28261 gaccgggcct acaattgtct gatgtgcggt cgttgcaaca cctattgtcc ggtgaaaatc
    28321 gatgtgacaa atatgcgcaa cgtcacgcgc aaacttgcca atgaaaccgt ttctttccgt
    28381 tatgactatc tgccggaaat caaaaaggac gagttggctg aagtgacgaa tgtggtttat
    28441 tttgcgggct gtatgtcgca tctgacgcca tcggtgccgc gtgctatggt tgccttgttt
    28501 aaagaagccg gcattacata caattttgtt gatgaaaatg gaagtatgtg ttgcggccgt
    28561 ccgttgatgc agtcaggact gactgaagca gctgcggagt tgagggctaa actcacaaat
    28621 ctgattaatt caaggaattc aatgattctt gtgaccagtt gtccgatatg ctacaaaaca
    28681 tttaaggaag attacaatct gaaaatgcat gtggtacatc ataccgagtt gattaaaatt
    28741 ctgattgaga gcaagcgttt gaaaccgcgc atggaagatc tttccattgt atatcacgat
    28801 ccatgcgaac ttggccgtgg atgcggtgaa tacgaaaatg cacgtattgt attgaatcag
    28861 attggaagca ttgtttccat cgatcaggaa aaggaaaaag cgctgtgctg tggtggcagt
    28921 atttccaacc tgaaagtgcc gtacgacaag cgcgagttga tggctgccga tgccgtgaaa
    28981 caattgtcgg cacccaatcc tgatgtgatt gtaacggcat gtccgctttg taaaaagacc
    29041 atggccaaat atgccgacgg caaaatgaag gacattgccg aagtggtggc tgaggctgtt
    29101 ggtgcagtaa agcaggcaaa taaaattgaa gaaaccgaag aagttcaggt ggtatagtgg
    29161 attcttctga tttcatcatt tatactcatt gagcagcgaa gccttgagca aaacgagaca
    29221 atgtttgagt aaatacatgg ctatatcgtc gatgaattca ggactttcat aaccaccact
    29281 ataatcggct gcaatttctc tctgatacct gagcacaatc ggaaataaat tttcgttcat
    29341 aagttgctga taatcctcaa gcgttgtttt atcagaattt tcaagaattc cgcacatttc
    29401 ggaagatatc agccggagcg attctttccc gggaaccgaa gcgaatgttt tgtattgcgt
    29461 aatcagaata aatgattcac aattaaatat cagatttttg gctaatcgtg agctgaaaat
    29521 ccccataact gaagtttcat ccagaccagg aatttccatg cgaatagatt tttttagtga
    29581 atcgcagatt tcgggatgat gaagcatagc cgataaagtg taggcctgtt gcatttgcat
    29641 ggccgtcgca ctgtccttgt ccatgcatct ggccgagatg gaatcgcata tttcagcagc
    29701 aataacatcg tatccgcttt gagcaaacga ttgagataca aataaaaaaa taaaaacggg
    29761 aatcagaaaa gctttcatgg tatggattaa ataaaacaca aagttaaaaa aacaggagaa
    29821 tttcattttt cagtttatgt tgaagtcaaa aatacattca acagatttat acttttctta
    29881 aagaaaggta tcaaagagca aatttctgca ggtttgtata tttgcagaaa aaaagacgta
    29941 agttcttaag cgaagaaatt ttaaatgatt catgtgaaaa aacaatgcga agactgtgct
    30001 ttcagaaatg aaatatgtcg gtttattgac gaggaagaat tttcggaact aaatgaaacg
    30061 actctttatc agcattcgcc aaaaggtcag accatcgtca gacagggcga tccgatgacc
    30121 catttgctgt atttatcgtc gggactggtt aagcttgagc atgtttcgcc caataaacag
    30181 atacagattc cggcattgat aaaaggccct gcactgatca gcggtaatta tcagatcagc
    30241 ggtgcgaata acctgttatc ggtagtagct gttgaagaaa ccaatgcctg cctgattgat
    30301 ctggtggtac tgcaaagccg gatgcgaaaa aatgccatgt ttgccatgac catatatgat
    30361 atgctgacag catttcatca gcgtgtgctt gacaaacagc tcatgttggc ctgcaagcgg
    30421 gttccggggc gcattgccgg gattctgctt ttattccgcg aaaaatttta caactccaat
    30481 gaattttcat ttccgctgac ccggcgcgaa atttcgcatc tcacatcatg cagcgaagaa
    30541 aacgtaatac gcacgttgag tagttttgag caggatggaa ttataacttt gagcgggaag
    30601 tcagtaaaga ttcttcatcc ggaaaagctg aagagaattt atgatattgg gtagtaaaaa
    30661 tccctcgaag ggtttcaaac ccttcgaggg attgtttata aaaaaatcac aaccttttca
    30721 gattgtgatt ttgcagtatt ctttgtatta agtattacca gcgttttggt ttgctatcgg
    30781 agttataacc accacggcca ccgccacggt ttccgccgcc gctgtaacca ccacggtttc
    30841 caccgccacc gaaattgcct ttcggacggt cttcgcgcgg acgtgcttcg ttaacagaga
    30901 tcactttgcc gtcataagtg gcttcatgaa gttctttaat ggctttggct gctgcttcgt
    30961 cattgggcat ttcaacaaaa ccgaagcctt ttgaacggcc ggtttcgcga tccatgatga
    31021 ttttggcaga agttacttcg ccatactcct cgaaaactga ttttaagtct tcgtctttca
    31081 gacgataact caggtttgaa atgtaaatgt tcattgtttg tttttgttga taatagtgtg
    31141 tttccgttga aagcgatgaa acctacagca ctattaataa caacaatgac aatcagttta
    31201 tgtctttcaa cgcgtttgca aaggtaagaa ttttctctca atatcaccat attttttttc
    31261 aaagatgtta aaaatgaggg gatttgcatt aaaaaccggg aattaaattg aacaaaacgt
    31321 ccgtatccgc tggcgaaccg accttcaaga gttcgagatg caatgaagaa cctggaagag
    31381 tcggatgtgt actatggcgt taatgcgatt atgtgttaat gcgataatgc gaatttggtt
    31441 tgccgcatca tcgccaacta ttccatgttc tccgattctc ttcaaactat ggaatgttgg
    31501 attgactgta cttgttttga gaaatcctct aaaacgccca ttttgcagaa tctgccaaaa
    31561 attctacctt tgtaactaaa tcgattgata tgaagacttt agttttatta cgtcacggag
    31621 aaagtgaata caacagccag aaccgcttca cgggctggac tgatgtgaat ctgagcgaaa
    31681 aaggaattac cgaagcccgc actgctggca aagcattgaa ggaaaacggt tttgaattcg
    31741 atattgccta tacttctgtt ttgcgccgcg ccataaaaac actgtggctg gcacttgaag
    31801 aaatggatcg tatgtggctt cccatcaagc attcatggcg tctgaatgaa aaacattatg
    31861 gcactttgca gggtctcaac aaatcggaaa tggcagccaa atacggcgaa gagcaggtgc
    31921 ttttgtggcg ccgcgcctac gatgtgcgtc cgccgctgta cgatgaagcc gacgtaaagc
    31981 catttctcaa tgaagcgcgc tataaagatg ttgacaatct gatttacatg cgtggcgaat
    32041 gtttgaaaga taccgttgaa cgcgtgattc cgctctggga aaatgaaatt gcgccggcga
    32101 tcaaaagcgg caaacgcatt atcattgcag ctcatggcaa cagtctgcgc gctgtggtta
    32161 aatatctcga aggcatgagc gacgaagcca ttctgaaata caacattcca accggaatcc
    32221 cgcttgtat
//
LOCUS       DAOPKE010000027        31922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1485_length_31922_cov_13.688424, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000027 DAOPKE010000000
VERSION     DAOPKE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_31922_cov_13.688424"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="PKY63_05250"
     CDS             complement(<1..2124)
                     /locus_tag="PKY63_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00058.1"
                     /translation="MKNIYKMNGLRIQFGRLPARLSAWLFIVATLLVTPVSTSAQSGL
                     CDPTVPFFTVDLTASPNATWISPLIVRNDNCCGTSSPDRCLEFEISVNPGTIAINFDI
                     ASGAVPPGAMFYQIDCGPPTPVGTPICLTGTGPYTLTFCKPGNNANTYAVTAIPEPAV
                     GPSVYASSNCPAGIWVSGLVPGTISWTDVTGGGLYNSYLSCTDCEDPSVTPAPGHPAY
                     VDYVACGTIAAGACSGSSSFCDTVRVYMFDPIDAVVNPNPAYFCPSDPGVQLNGSHSG
                     GYGTVVYTWYNSSWTQVGTGNGYFATSAGTYYLVVTDDMSAVCPADTVPVTVNVYDPP
                     ILVSPSYAGICEGSSVTLTASGADSYVWAPTTGLSPATGSVVTAAPATTTTYTVTGTD
                     SHGCTGSMTVLIDIFPLPNIDAGPDQEICIGESAQLQGSGAFSYEWTPAATLSNPNSS
                     NPIATPATTTTYYLTGYSIGGNLIYNGDFELGNTGFTTDYIYDPNLFPEGNYYITNNP
                     NTHHSNFAPCADHTPAPGSQMMVVNGAPNANEQIWCMNVAVIPNTDYAFSTWITSVHP
                     TNPAVLQFSINSILLGSPFTATSTTCDWQQFYEIWNSGTNTSAQICIVNQNIIRNGND
                     FALDDIGFAPLCSNIDSVTVTVNPNPILTITATNPDICTGNSTTLSASSDIPGTTYVW
                     SNGATTNSITVSPASTSTYFVTGTSA"
     gene            2606..3172
                     /gene="efp"
                     /locus_tag="PKY63_05255"
     CDS             2606..3172
                     /gene="efp"
                     /locus_tag="PKY63_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPF00059.1"
                     /translation="MATTADIKNGLCISFNNDLFVVVEFQHVKPGKGAAFVRTRLKSL
                     TTGRVIQNTFPSGFKIDIQRVERRPYQYLYKEDKTYFFMHQETYEQISLEEELINAPQ
                     FLKEGQGVEVVFHADTESPLSCELPPFVNLEVTYSEPGVKGDTATNTLKPATVETGAS
                     IGIPLFVNTGDKIKVDTRTGEYSERVKE"
     gene            3189..4130
                     /locus_tag="PKY63_05260"
     CDS             3189..4130
                     /locus_tag="PKY63_05260"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPF00060.1"
                     /translation="MKFSKNYTLQQMVDIIGVNTVQMKGAPDFAVTGLNEIHSVEPGD
                     LTFVDHPKYYTKALDSAATTVIINKADVDVPAGKALIVSDDPFRDYVKLVKHFKPFIP
                     SQSSISPSAEIGEGTIIEPLVFVGHNVKIGRNCIIMANATIHADTVIGDNVIINSGSV
                     IGGEAFYFKRRPDFLDKLEGCGRVIIEDNVEIGSCCTVDRGVSGDTTIGCGSKLDNHI
                     QVGHDTIIGRNCLISSQVGIAGVVKLEDDVILWGQVGVQKDLVIGKGAVVLGQSGVAK
                     SIEGGKTYFGSPVRDAVDKMKELALIKRLPEVFEKLK"
     gene            complement(4181..5671)
                     /locus_tag="PKY63_05265"
     CDS             complement(4181..5671)
                     /locus_tag="PKY63_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPF00061.1"
                     /translation="MNTGAVKSIHTSKTERWVLALSLIFLLINLAAILFKENFYVSLL
                     PVFLGLIYLYFFSVDTIFLIIAFTSPLAINLSDWDARLAVSLPTEPLLAGLLLIFLLK
                     SFYELNQWKAFLRNPVTIAFIAYFIWIAFSSLTSRMPLVSWKFFASHLWLIIPMYFFG
                     TRLFFLKESTQRNFVIVQFIALTFVAIYTLIRHAGYNFGDDESHWVMQPFYKDHAIYG
                     SALALFIPVIFFLFTDKKYTFTQKFFLMTSIVIMTLALLFSYSRAAWLGFFIAIGIGF
                     IVLFRIKFKVIIVGFLFLLLAGFFFQTQIMHRLEKNKQDSSEDIVENIESISNISTDA
                     SNLERLNRWNCAIRMAADHPVCGTGPGTYQFLYAPYQFSYEKTIISTNAGTLGNAHSE
                     FLGALAETGIPGMLIVIFLVFSIFFYGFQTYSKLRKTDREKARFILGLLLGLVTYFAH
                     AFLNNFLDSDKIAIPVWGFVAIIVANGLAIKQKSKVKEVTKEKNTD"
     gene            complement(5684..6715)
                     /locus_tag="PKY63_05270"
     CDS             complement(5684..6715)
                     /locus_tag="PKY63_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00062.1"
                     /translation="MKESTTPKELTFENTNFLFFLFKWRKLLIIICIAAAVISAGISL
                     LIKNKYVSSVVLFPTSTNAVSKALISSQYGAKEDIMAFGEEEEAEQMLQVLNSNEIRQ
                     RIIQKFNLLEHYDIDPESKFKNTRLYETYNENISFNRTEYMAVEIKVVDEDPQMAADI
                     ANEIANLYDTVKNDLQKQRSLEGFKIVEGAYKELEADIIRKEDSLSILRSKGVQDYES
                     QAERLYEGLAREIGNGNQRAVSQIQERLDTLAKYGSAYVSLRDGLEHDKKQLSDLKGK
                     YEEAKVDAESSLPQKFVVDYAFKAEKKSYPVRWLIVVISTFATLLTSIILIILYENFR
                     KFKLLSATE"
     gene            complement(6770..7309)
                     /locus_tag="PKY63_05275"
     CDS             complement(6770..7309)
                     /locus_tag="PKY63_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPF00063.1"
                     /translation="MEKRLNLGCGHDIREGWINLDISPIEGVDVVHDINKLPLPFDNE
                     SMDYILCQDIFEHIEYIPLLEELHRILKKGGVIEIRVPHFSSRFNFIDPTHKKMFSIQ
                     TFDFFTKHAPFERSYYFPFHFEKTIYRKIAFSKSWVFFINFLIEPFINCSQRMRTFYE
                     ASFLSRLFPCANIIVRLKK"
     gene            complement(7294..8751)
                     /locus_tag="PKY63_05280"
     CDS             complement(7294..8751)
                     /locus_tag="PKY63_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPF00064.1"
                     /translation="MQKKFIINLFFLIGINLLVKPFWIFGIDRTVQNVVGAADYGLYF
                     AVMNFSFLFGFMLDFGLTGFNNRSVAQHEHMAGKYTGSILGMKSALALFYALISFVAA
                     FLIGYDTLHFKLLIWLGINQILSSFILFLRSNISGMQMFKTDSLLSVLDRLLMIVFFS
                     VLLWGNVFDISIDIFYFAAFQTLSYLITFLIALFIVLRKIKLSKIKWNTKLNIVLFRQ
                     SLPFAVLTFLMLFYYRVDSVMIERLLPQGSMHAGIYASAYRILDAFGMFSYLFSVILL
                     PFFSRLMSKKQDVGPFMQLSAILLLSSAMMVSAVSLFNADLIMNWLYKEHVTESARVL
                     QILMFTFIPVSSVYIFGTLLTAWGSMKKLNIIALCGVILNIGLNLLLIPQFLERGAAW
                     SALITQSLVAISQIVLSLMLVKPSGLKKTMVLLTVFALAIYPVAWITDILQMKPGAGI
                     IIIAAVWILFLLVNTLLLFRRNKLTISDLLAWRKD"
     gene            8800..9963
                     /locus_tag="PKY63_05285"
     CDS             8800..9963
                     /locus_tag="PKY63_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPF00065.1"
                     /translation="MNRTEHKPVIAINTRHWIPGRMEGIGRFEMNIATELSRLHPEAE
                     FHWFFDRRPKLDMAVPENVKIHVLFPPARHPRLYYIWYEWSVPMMLKKIKADIFISAD
                     NINSLKAHCPTITAIHDLNFVHYPYDLPLTYSSYYNTKTSRFISKAIKVITVSEFSRN
                     DIIEQYKTPENKIHVVYNGVASHFAPLNSWVISETRFKLTSGKPYFFVLGSIHPRKNL
                     AKTIEAYTLFRKQTNCDYPLVVSGRELFLNKNLGNILNNNPYRKDIVFTGYLDNKALG
                     ETLGSAFALLYMSVFEGFGVPVIEAFASCVPVVASDVSSIPEVAGGAAMLANPHDAGE
                     VADAMKKLSEDEQLRISLCEKGLKRATAFSWKESAERFWKVIQEVLAETKIKS"
     gene            complement(10522..11169)
                     /gene="elbB"
                     /locus_tag="PKY63_05290"
     CDS             complement(10522..11169)
                     /gene="elbB"
                     /locus_tag="PKY63_05290"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPF00066.1"
                     /translation="MKKIAIILSGCGVYDGTEIHETTMSMLAIDQQGAQYELFAPDID
                     QHHVINHLTGKQNQETRNVLVESARIARGKIKNIKEFNVAESDALLFPGGFGAAKNLS
                     DYAFEGAAMKVNADVEKAILSMHAAGKPIGALCIAPVLLAKVIEGCNVTIGNDKDTAT
                     HIEQMSSQHSITSATGIVIDEKNKLVTTPCYMLGTRISEIYEGARKVVDALMKMI"
     gene            complement(11246..11692)
                     /locus_tag="PKY63_05295"
     CDS             complement(11246..11692)
                     /locus_tag="PKY63_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00067.1"
                     /translation="MKSMTKILMAVVAIAFVAASCGKYEEGPAISLASKEARVINVWA
                     LDAEYENGVEQTLTADDKDDYIEFMKDGKAVFTWVTSSGNATFDATWELSDDKEELVY
                     YMTYELMGVTYTDTTESTILKLKSNEMWLEQVDGSDKTEYHYITKE"
     gene            complement(11747..12181)
                     /locus_tag="PKY63_05300"
     CDS             complement(11747..12181)
                     /locus_tag="PKY63_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPF00068.1"
                     /translation="MSKITALIVLIAFVAVSCGKYEEGPKFSLASKNARLINTWKIDE
                     IYKNGASQTVTADQKDDYVEAKKDGVLSVTYVSSGFTTTYTGTWEFTADKANLRLTYT
                     GSLLGIPFTSDEEYTILRLTSDELWLEQVEGSNTFEYHYITK"
     gene            complement(12345..13103)
                     /locus_tag="PKY63_05305"
     CDS             complement(12345..13103)
                     /locus_tag="PKY63_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPF00069.1"
                     /translation="MEIQLVIICVVSFFAGFIDSIAGGGGLIQTPAILFLFPQYPVAT
                     LLGTTKIPSFSGTALATYQFSKKATINPKLFLFLVPAAFAGSALGAWCITIIEDDFIK
                     PVILALLVVVAVYTFLKKDFGSVKTKDVELKRQIVAGTLFGFIIGFYDGMIGPGTGSF
                     FILCFIFFMGFDFIAASANAKMLNLATNLSAIALFAATNNIIYRLAIPMAICNMGGAY
                     LGSKLALLKGNRFIRIFFLIVVTATIVRFAFEVF"
     gene            complement(13290..14675)
                     /gene="gatD"
                     /locus_tag="PKY63_05310"
     CDS             complement(13290..14675)
                     /gene="gatD"
                     /locus_tag="PKY63_05310"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPF00070.1"
                     /translation="MSEDFFQGYKGHALELLKKYQVRVWGQAEVETTRGNFQGTVLPR
                     AENDDDKHIVLKIITGYNVGVDTSTILNMKETGYKKANYKIPEKQFPVTKGLPNVKLL
                     GTGGTIASRLDYRTGAVIPAFTPGELYGAVPELADICNLTTDKLFAVFSENMGPEQYK
                     KLAIAIGEEIEKGIDGIVIGHGTDTLSHTAAALTFMVQNSPVPIVLVGSQRSSDRPSS
                     DAALNLMHAMTAAGHGDVAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTLSD
                     VPLAKITRKGVVPIKQNYNPRRKDRKVNIYPYFEEKVAIVYYYPNMMPDIIDSLVDNG
                     YRGIIIAGTGLGHINKPLYPAIERAHAKGVHMFMAVQTLYGYCHMFVYDTGRDLMAKG
                     IVPAANMLPETAYIKLGWVLGQTDDPEEVKRMMLTPVNDEITEREPYNGYLVLQGGVK
                     EVDDFVKAFHK"
     gene            complement(14675..16654)
                     /gene="gatE"
                     /locus_tag="PKY63_05315"
     CDS             complement(14675..16654)
                     /gene="gatE"
                     /locus_tag="PKY63_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPF00071.1"
                     /translation="MRTDFDARANYENTKKEVGYVPRREATQATYDSIGFMSGLEVHQ
                     QLLTEKKLFCNCPAGKFNPSYIYDAEVIRHMRPTLSELGEYDGTALMEFKTRKEIVYR
                     VKNETTCTYEVDDTPPFPINRQALDIAIKISLLSKLNIVGEVHITRKQYLDGSIPTGF
                     QRTAIIGVEGELPMKDKKIRLIQLSIEEDSCREISDIGHTRVYKTDRLGMPLIETVTY
                     PDMKNPDEVKEVCDYIRFLNRSTGLVRTGIGAGREDVNVSCKGGTRIEIKGVSHTKWI
                     PELTHVECFRQWALLKVRDKLLKKFGSASNWSMRHEFLDYDMFQITYEPLKVARDAGH
                     KIVAVNLPGFRGILSHFTQPTKMFANEISDRLKVIACLEKPNMLHTEQFKPIITPLDL
                     EIIAPMLNAKDDDAQIVFWGPEADIKTALEVIEERCRMAFEGIPNETRKSFEDGSTIF
                     ERVLPGADRMYPDTDSAPIPLTDQHIEELRKGLPNEVSERYNQLKKWGVAESTYYYLF
                     TNNLYPAIDAISKNTDTDPKWVGNFIGHRLKWVEGHFEKGPGFSISNIEKMIQYLKKN
                     HMDLRYAYNMLPVVYQYPKMDFDSVLTSLNFKLLKADEITEKINFLNEKFGTVCHTDT
                     PENRINWIMGELQRIARGNMSLTELHTIVKQKISK"
     assembly_gap    16756..16855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17183..19222)
                     /gene="metG"
                     /locus_tag="PKY63_05320"
     CDS             complement(17183..19222)
                     /gene="metG"
                     /locus_tag="PKY63_05320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPF00072.1"
                     /translation="MKDYKRYTVTTALPYANGPIHIGHMAGVYVPADIYVRFLRKTGR
                     DVLFIGGSDEHGVPITIKARQKGITPQQVVDEYHGIIKKSFEDFGMSFDVYSRTSLPV
                     HHETASAFFSKLHAEGKLTQMESEQYYDEEAKQFLADRYITGTCPHCGNERAYGDQCE
                     KCGTSLNATDLIDPKSTLSGSAPVKRKTSHWFLPLDQYENWLREWILEGHKEWKTNVY
                     GQCKSWIDQGLQPRAVTRDLDWGVKVPLADSEGKVLYVWFDAPIGYISASRDWSQQTG
                     RSWEEYWKSDDSRLIHFIGKDNIVFHCIIFPAMLHAEGSYILPENVPANEFLNLEGDK
                     ISTSRNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELAAIF
                     GNFVNRAMVLTHKYFEGRVPASTQGAELDDIMKEVSALKEKITGSLEQFRFREALAFF
                     MDIARLGNKYLTDKEPWKIYKENPEAVKGILNNVLQISANMAVLSEIFLPFTATKLKA
                     MLNMNETSWNNIGKPMLDEGLQLNQPEILFEKIEDADIQKQLDKLEASKIANKAANVS
                     FEPVKSDVSFDDFSKLDLRVGKILEAEKVAKTKKLMKLSVDLGFETRTIVSGVAEYFT
                     PEQLIGKKVVVVANLGKRDIKGVESNGMILFAENADGTLLMVNPCDEAICGGMVK"
     gene            complement(19288..20064)
                     /locus_tag="PKY63_05325"
     CDS             complement(19288..20064)
                     /locus_tag="PKY63_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF00073.1"
                     /translation="MDTHLFGSIIFGPVRSRRLGLSLGINLLPANKKICTFDCVYCEC
                     GWTDKTSADGLPKATEVIAELEKKLQEMQAAGELPDAITYAGNGEPTMHPEFDFIMQE
                     SVRLRNQYAPAAKITVLTNSTQLHKQKVADAVALADKGLLKLDSTDPKQFELINRAAK
                     GIELSDVMHFIELFKGEKIIQTMMLRGESEGEKIDNTTEESLLKLAAFVSKINAIEWM
                     LYPIDRPTPEKQLEKMSREEMDRIGEFVRKHTSVPVTIRY"
     gene            complement(20065..20400)
                     /locus_tag="PKY63_05330"
     CDS             complement(20065..20400)
                     /locus_tag="PKY63_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00074.1"
                     /translation="MANKRGIKKDVNYLTNEVLVDGIMLMSLYNEKDGELIMKELEKV
                     AEKRNQVIDTLQHAEKKFERLPKEERKNGRIARSKAFRKVVNEKFASFEDALDAAYES
                     FGKLSKEEK"
     gene            complement(20404..22305)
                     /gene="dxs"
                     /locus_tag="PKY63_05335"
     CDS             complement(20404..22305)
                     /gene="dxs"
                     /locus_tag="PKY63_05335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPF00075.1"
                     /translation="MVVKAGTYLSRIDGPEDLKKLSPQELIMLSQEVRDFLIENMARH
                     PGHLGANLGAVELTIALHYIFDSPKDRIIWDVGHQSYIHKILTGRRDVFHTIRQKDGI
                     SGFPKISESEHDIFGTGHSSTSISAALGMAVGEQDSGINANHYIAVIGDGSMTGGEAF
                     EALNNIGALKNNILVILNDNGIAIDENTGSFTRYLTRITASHTYNRLKQSIWRIFKGT
                     FVQKIANKTSTALKWTWLKKSNLFEAFNVRYFGPVDGHNIKQLINVLEDLRDINGPKL
                     LHVITKKGKGYKPAEKDQVTFHAPGRYDAKTGEILETSCDSVPPKYQVVYGKTLTELA
                     EQNENICAITPAMITGSSLNIMQNRFPERVFDVGISEQHAVTFAAGLAKSERIPFCTI
                     YSTFLQRAYDQLIHDVAIQNLPVIFGIDRGGLVGEDGATHHGIYDLAYLRAIPNIIIA
                     SPINAIQLRNLMFTAQSEKHGPFAIRYPRGNSEVKDWKQSFVELQIGKSEKLCSGNDV
                     AILTIGQPGNDVISLYSKLSEAGISVSHYNMIYLKPLDEDALHHACKNHKAIITIEDG
                     TVKGGLGTAVAEFMMEHSYYLPFIKLGVPDRFIEHGTIAELKKECGFDKDGIFSAIKE
                     IFSKTNQTK"
     gene            complement(22698..23981)
                     /locus_tag="PKY63_05340"
     CDS             complement(22698..23981)
                     /locus_tag="PKY63_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPF00076.1"
                     /translation="MRYKPMSPEFFKRNRKNLIEKLQPESIVVLAGNEHMPWSGDQDY
                     PFRQNSDFYYLTGIDEENALLCLCPHHPDPNLREVLFIQEADPTMVIWYGKRLSREKA
                     SELSGIKTVKWAADFKAALRDMTQQSRNIYMGLNEYLKYSTNTHDANRRMIDEIKNLF
                     PLHQYERLTPLTTELRLCKSQEEIDMIGKAIGITKEAFTRVLKTTKPGMMEYEVEAEI
                     THEFIRRGASGHAYSPIVAGGENACVLHYITNDCPLKDGDLMLLDFGAVYGNYAADCS
                     RTIPVNGKFTPRQKQCYNAVLDVLKEAKKILVPGTTIEKTNKQIALMLEQKMIELGLF
                     SVDDVKNNTTSTPLYFKYYMHGASHFMGLDVHDVGLRTMTLKKGMVLTCEPGIYIQEE
                     SIGIRIENDILVDDEPINLMTEIPLEADEIEKLMR"
     gene            24799..25479
                     /locus_tag="PKY63_05345"
     CDS             24799..25479
                     /locus_tag="PKY63_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF00077.1"
                     /translation="MTQKRFLCVKSIYLCGMEEKLKYILEEASKHFMRYGIRSMSMDD
                     IARELGISKKTLYQYFADKKELVHSVMKFHMDYTNDCFRNMKETPGNAIDILLRVSEI
                     LIEQLGQMNPSVKYDLSKYYPEAFKTLMDYNRGQVLDHIERNLMQGIKEGLYRKEMNT
                     RIIAYFYLIRMDQVMAMEPDDEKMKSITTEAILNELFVYHVRGIANEKGIEYLENTLM
                     KDLKNKRK"
     gene            25522..>26779
                     /locus_tag="PKY63_05350"
     CDS             25522..>26779
                     /locus_tag="PKY63_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPF00078.1"
                     /translation="MKQTIILLTLFLFLCNHGSAQEKLSLTLGQAQEYALDHNRTLKN
                     ASLDIQKAEANKWSTLSTMLPQVNGSVDYANMMGYQLTMMGFDIAMPPYATIGVTTSI
                     SLSASQVIGVQMQNIAAKMADITLKQTEQQVTNQVKTLYFSALVMEETTVLLDSNLAN
                     MKKLLAHTENSVKVGILNQTDADQLAVQIVTVENTINSVKRSLEMIYNALRLQLGISV
                     DTEIELTQAIDELVNIEKANSLLSVGFSSENSFSYQLLKSSVDLSKAQVNMKKWAYAP
                     SISAYHSYTKKEYFSDEPTMNMTPPNIIGLSLKVPIFSSASRYASLKAAQIDYEKQLN
                     TLADAGEGLVIQHRQLCFNLSSALETYETQKRNIEVNQRIFEDISRKFENGMASSLEV
                     TTAGSGLITAQSNYVQTLLNLVSAQIA"
     assembly_gap    26780..26879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26967..28016
                     /locus_tag="PKY63_05355"
     CDS             26967..28016
                     /locus_tag="PKY63_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPF00079.1"
                     /translation="MKTIKKQNTMNRLFQTIIVAGLAMLVVSCGTKGKSEEQVVVENK
                     VEKVKVEAIQKQTVARKLDLSTTLEGYETMKVSPSLTGNIEHIYVEVGSYVGAGQMLV
                     RMDQNKYNTAKLGFTQASIEFERVKALKETNTVSQQTYDQAKLGYDQAKESLNFLTEH
                     TFVKARFPGVVSAKNYVDGELYSGAPILVITQINQLKALVSIPETYFPLIKTGMTMNL
                     RSDIYKDETFPASIEIVYPTIDPATHTFQAKLRIPNGTSKLRPGMFVRTTLEMGEVEA
                     LLVPYQAVLKLTGSNERFIFVNENGVAKRVSVSLGQRYDDKVEIISDQVKEGDQLVIL
                     GQTKLVDGTKLEVIK"
     gene            28106..31303
                     /locus_tag="PKY63_05360"
     CDS             28106..31303
                     /locus_tag="PKY63_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPF00080.1"
                     /translation="MAIYKSAINKPVTTILLFVAILVVGLFSFSRLPIDQFPDIEAPY
                     ITVMTTYPGASSSEVETNVTKVLENTLNSVDDLDELTSTSKDNMSLIMLKLKWGTNLD
                     EVINDVRSYIDLTKDYLPTGCSNPFIFKFSTSMMPIIMFAVTADESYAGLDKILNDQV
                     VPQLNQVDGIGNISISGSPIRYVYIDLDQEQMDAYGISLEQVGSAISSNNLNVSSGTV
                     KMDKEQYALQVRSEYVESEEIADIVVKTTMDGRQVFVRDIAQVKDTIKDLTLDEKVNG
                     QNGVRLVIAKQSGANTVQVASDVQKQLENIKSALPSDIEISVIYDSAETIKDSIYSLE
                     ESIMFALLFVVLVVLFFLGRWRATLIIALTIPIALVGSFVYLAFVDSSLNIISLSSLT
                     VAIGLVVDDAIVVLENIIKHIERGSTPREAAVAATNEVWVSVIATTLVIIAVFLPLTM
                     LGGMAGIMFKELGWIVTIVIAISAIVAISLTPMLASRILRKKKVAVDSNGHIIEEKEK
                     SLWYDRSIGRMLHKLDLRYANILRWCLNHKKLTMLIVVVFFVVSLIPALTGQIGTDFM
                     QQSDGGRLTVTAELQRGTRLEETMKTARRLESRFMEIAPEIKIISTTVGSSDEATISA
                     MFSTATNNKISMTVVCSDKSERKRSIFEIAEVLRQEMLLYPELTTFQAQVAGMMGGGI
                     AGTSTVNVEIYGYDFDQTAVMAETVKKAISENVKGARDITISREPDRPELKVVVDKQK
                     LAMHGLSSSAVSMYVRNRVLGMNAGFLKEEGEEYDIVVRMKEENRNSISAILNLSIPT
                     PSGGKIKLSEIARVEEFWTPQTITRKSRQRIVTVAVTPYKTSLGELAAGVKDELKNIE
                     VPNGITLNIGGDYQEQQETFADMTLFMLLIIMLVFIVMASQFESFKKPFIIMASVFFA
                     FSGIILALFITGTTLDMIGALGAIMLVGIVVKNGIVLVDYTNLMRDRGYELKEAIALS
                     GQSRLRPVLMTALTAILGMVPMAISTGAGSEMWRPMGIVIIGGLTVSMFLTLIVVPVL
                     YGIMSRHGERDETERQRKEFIFMEISDETSTTSKN"
     gene            31308..31601
                     /locus_tag="PKY63_05365"
     CDS             31308..31601
                     /locus_tag="PKY63_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPF00081.1"
                     /translation="MKAVFIVFNQSHTERVEYMLDKLGIRGFTFLEDVQGRGNIDGEP
                     HRGTHTWPEMNSAVITIVQDNQVDDLLKSVHKLDLRNKAAGVRAFTWNIEQMM"
     gene            31761..31838
                     /locus_tag="PKY63_05370"
     tRNA            31761..31838
                     /locus_tag="PKY63_05370"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31796..31798,aa:Val,seq:cac)
     gene            31847..31921
                     /locus_tag="PKY63_05375"
     tRNA            31847..31921
                     /locus_tag="PKY63_05375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31882..31884,aa:Val,seq:gac)
BASE COUNT         8580 a         6605 c         7296 g         9241 t
ORIGIN      
        1 ggccgatgtt ccggtaacaa aataggtgct tgtgcttgct ggtgaaacag taatggaatt
       61 tgttgttgca ccattgctcc aaacgtaagt tgttccggga atatcagatg atgcagacag
      121 tgttgtactg ttgccagtgc aaatatcagg attggtggct gtaatagtca gaataggatt
      181 tggattaacg gtaactgtta cagaatcaat attagaacag agaggggcaa acccgatatc
      241 atcaagagca aaatcatttc catttcggat tatattctgg ttgacgatac agatttgtgc
      301 agatgtgttt gtgccactgt tccatatttc atagaattgt tgccagtcgc aggttgttga
      361 ggttgcagtg aaagggcttc caagaagaat gctgttgatg gaaaactgca gcactgcagg
      421 attggtggga tgcacactgg ttatccaggt agaaaatgca taatcggtat ttgggattac
      481 tgcaacattc atgcaccaga tttgttcgtt ggcatttggg gcaccattta ccaccatcat
      541 ctgggatccc ggagccggag tgtgatcagc gcaaggtgca aagttggaat gatgggtatt
      601 tggattgttg gtgatataat aatttccttc agggaaaaga ttagggtcat agatataatc
      661 ggtagtaaaa ccggtatttc ccaattcaaa gtcgccatta taaatcaggt ttccaccaat
      721 ggaatagccg gtcagataat atgttgttgt tgttgccgga gtggcaatcg ggttggaact
      781 attggggttg ctgagtgttg ccgccggagt ccattcatag ctgaaagcgc cgcttccttg
      841 taattgagcg gattctccga tgcatatttc ctgatcgggg cctgcgtcaa tgtttggaag
      901 aggaaaaata tcaatcagaa cagtcattga accggtacat ccgtgagagt cagtacccgt
      961 aacggtatat gttgttgttg ttgccggggc tgctgtaacc acagatccgg tggctggaga
     1021 taaccctgtg gtgggagccc acacataaga atcggcacct gaagctgtca gtgtaacgga
     1081 gcttccttcg cagatgccgg cataacttgg tgaaacaaga atgggaggat cgtaaacatt
     1141 gacagttacc ggaaccgtgt cggcggggca gacagcgctc atgtcgtcag ttactactaa
     1201 ataataggtg cctgccgatg ttgcaaaata gccgttacca gtgccaacct gagtccagga
     1261 agagttgtac catgtgtaaa cgacagttcc gtagccgccg gagtgcgacc cgttaagctg
     1321 aacgccagga tctgatgggc agaaataagc tggattggga tttacgactg cgtcaatcgg
     1381 gtcaaacata taaactctta ctgtgtcaca gaaactggat gatccagagc aggcgcctgc
     1441 tgcaatggtt ccgcaagcca cataatcaac ataggccgga tggcctggag caggagtaac
     1501 gcttgggtct tcgcagtcgg tgcagctaag ataagaattg tacaggcctc cacctgttac
     1561 atctgtccac gaaatggttc cgggaacaag ccccgatacc cagattcctg cagggcagtt
     1621 gctgctggca taaaccgaag ggccgacagc cggttcagga atggctgtaa cggcgtaggt
     1681 gtttgcattg tttccgggtt tacagaatgt aagagtgtaa ggcccggtgc cggtaagaca
     1741 aatgggcgtt ccaaccggtg taggtggccc gcagtcaatc tgataaaaca tagctcccgg
     1801 aggaacagcg ccggaggcaa tgtcgaaatt gatggcaatg gtcccgggat tcaccgaaat
     1861 ttcgaattcg aggcatctgt cgggacttga tgtaccgcaa cagttgtcat tgcgtacaat
     1921 cagcggcgaa atccaggtgg catttggact ggccgttagg tcaactgtaa aaaacggaac
     1981 cgtcggatcg cacaggcctg actgggcact ggttgatacc ggagttacaa gcagagttgc
     2041 aactatgaac agccatgccg acaatcttgc cggtagccgt ccaaactgta tccgtaaccc
     2101 gttcattttg taaatatttt tcatggctca atattttggt gttttcagct tcgggctgaa
     2161 acgaaaatat atccgtggtt gaatgactga agaactctat tatttgcggt ggcgaatata
     2221 gatatgatat tgccgcagac aataagttta atatccctga tgtgaattct ttcaggaaac
     2281 agaaaatgat ataaaaggat gctttgtaaa gatttaatca tgtttcccgt ttttagaatt
     2341 acattttcta aatatacgaa actgaagcgt aaaaatcaag gtattttata tattttagat
     2401 aaatacatgt attgaggtga ttaatcgtta aatttaatca gaaaataaga atatttaagt
     2461 tgcctttttg cataaaacag gccgcatttc aaataaaagt gatttttcat aaaaatgagt
     2521 cagaagtcga ataatcagcg taatatgtat aatggtgttt gtagaatgca caaaatgtgt
     2581 aattttgcag ttgaaaaatc atcagatggc aacaaccgca gacataaaaa acggactttg
     2641 tatttcattc aataatgact tatttgtcgt agttgaattt cagcatgtta aaccaggtaa
     2701 gggtgcagca tttgtgcgta cccgattgaa aagtcttacg actggccggg ttattcaaaa
     2761 tacgtttcct tccggattta aaattgatat tcaacgagtt gagcgtaggc cgtatcagta
     2821 tttatataag gaagacaaaa cttatttttt catgcatcag gagacttatg agcagatttc
     2881 gcttgaagaa gaattgatta atgctcctca gtttttgaaa gaaggccagg gcgttgaagt
     2941 tgtttttcat gcagacaccg aatctcccct gagctgtgaa ttgccacctt ttgtaaatct
     3001 ggaagtaact tatagtgagc cgggagttaa aggtgatacc gcaacaaata cccttaagcc
     3061 agcgactgtt gaaaccgggg ccagtattgg cattccgctt tttgttaata ctggtgataa
     3121 aattaaagtt gatacccgta ccggagaata ttccgaacgt gtgaaagaat aaactgtaaa
     3181 attgttccat gaaattctcc aaaaattaca cattgcagca gatggttgac atcatcggag
     3241 tcaatactgt ccagatgaaa ggtgcgcctg attttgctgt aaccggcctc aacgaaattc
     3301 atagtgtgga gccaggtgat ctgacttttg ttgatcatcc gaaatactat acaaaagcat
     3361 tggattcggc tgcaacaact gttattatca ataaagcaga cgttgatgtt cctgcaggta
     3421 aagctcttat tgtttcggat gatccattcc gtgattatgt caagctggtg aaacatttca
     3481 aaccttttat tccgtcacag tcttctatta gcccaagtgc tgaaattggt gaaggaacca
     3541 tcattgaacc gcttgttttt gtcggacata atgttaaaat cggtcgtaat tgcattatta
     3601 tggccaatgc tactattcat gccgatacgg ttattggcga taatgtgatt atcaatagtg
     3661 gatctgttat tggtggtgag gcattttatt tcaaacgcag acctgatttt cttgataaat
     3721 tagagggttg tggccgtgtt attattgaag ataatgtcga aattggctcc tgctgcactg
     3781 ttgatcgtgg cgtatcgggt gatactacca ttggttgtgg atccaaactc gataatcata
     3841 ttcaggttgg acacgatacc attatcggtc gtaattgtct gatatcttct caggttggta
     3901 ttgccggcgt ggttaaactc gaggatgatg tgattctttg gggtcaggtt ggtgtgcaga
     3961 aagatcttgt gattggtaaa ggtgcggtgg tgcttggaca atcaggagtg gccaagtcca
     4021 ttgaaggcgg taaaacctat ttcggaagcc cggtgcgcga tgcggttgat aaaatgaaag
     4081 agctggcgtt gattaagcgc ttgcccgaag tttttgagaa actcaaataa gtgtaaaata
     4141 attgacccgg aacgataccg ggtttttttg catgcagtaa ttaatccgtg tttttttctt
     4201 ttgttacttc ttttaccttg gatttttgtt ttattgcaag tccattggct acaataatgg
     4261 ccacgaagcc ccaaactggg atggcaattt tatccgaatc aaggaaattg ttgagaaaag
     4321 catgagcaaa ataagtgacc aatcccaata aaaggccaag aatgaaacga gctttttctc
     4381 tgtcggtttt tcgtagtttt gaataggttt gaaatccata aaaaaagatg gaaaaaacca
     4441 gaaatataac aatcagcatt cccggaatgc cagtctcagc caatgctccg agaaattcgg
     4501 aatgagcatt tccaagagtg ccggcattgg tgctgatgat ggttttttcg tatgaaaact
     4561 gatagggagc atacagaaac tgataggtgc cgggtccggt gccgcatact gggtgatcgg
     4621 ccgccatgcg tatggcgcag ttccagcggt ttaagcgttc aaggttgctg gcatcggttg
     4681 atatgttgct gattgattca atgttttcaa caatgtcttc ggaactgtcc tgtttgtttt
     4741 tttcgagccg gtgcataatc tgggtctgaa aaaagaatcc agccagcaat aaaaaaagaa
     4801 atccaacaat aatgactttg aatttgatgc ggaacaacac gataaagccg atgccaatgg
     4861 caataaaaaa tcccagccag gcagcacggc tgtaagaaaa aagcagggcc agcgtcataa
     4921 tgacaataga agtcattaaa aagaattttt gagtaaaggt gtatttttta tctgtgaaaa
     4981 gaaaaaagat tacaggaata aataaggcaa gtgcagagcc ataaatggcg tggtctttat
     5041 aaaatggttg catgacccaa tgactttcat catcgccaaa attgtagccg gcatgcctta
     5101 tcagtgtata tatagctaca aatgtgagcg caataaactg cacgataaca aaattccgct
     5161 gtgtagattc tttaagaaag aagagccgtg ttccgaaaaa atacattggg ataataagcc
     5221 acaaatgcga tgcaaaaaat ttccaggaaa ccaagggcat gcggctggtg agactgctga
     5281 aggcaatcca gataaaatag gcgataaaag ctatggtaac cggattgcgc agaaacgctt
     5341 tccattgatt taattcataa aatgatttca atagaaaaat caaaagaagc cccgccaaaa
     5401 gtggctctgt cggcaaactt acagccagtc gcgcatccca gtcactcaga ttgatagcca
     5461 gtggtgatgt aaatgcaatg atgagaaaaa tggtatcgac agaaaaaaag taaagataaa
     5521 tcaatccaag aaaaaccgga agcagggaaa cataaaagtt ttccttgaat aaaatggctg
     5581 ccagattgat taacagaaaa atcaatgaca aggccaaaac ccatcgttcg gttttagatg
     5641 tgtgaattga ttttactgct cccgtattca ttgatattat tgattattca gttgctgaaa
     5701 gcaatttgaa ttttctgaaa ttttcgtata gaataatcaa gatgatagac gtgagtagtg
     5761 tggcaaacgt ggatataaca acaatgagcc agcgaaccgg atatgatttt ttttcggctt
     5821 tgaaggcata gtcaactaca aacttctggg gtagcgatga ttctgcatct actttggctt
     5881 cttcgtattt tcctttcaga tcgctgagtt gttttttgtc atgttccagc ccatctcgca
     5941 gtgaaacata ggccgagccg tattttgcga gtgtgtcgag tcgttcctga atctgactga
     6001 cagctcgttg attcccattt ccgatttcac gggcaagacc ttcgtagagt cgttctgcct
     6061 gcgattcgta atcctgtact cccttgcttc gtaaaataga aagtgaatct tcttttctga
     6121 taatatcagc ttcaagttct ttgtatgcac cttctacaat tttaaaacct tcgagtgaac
     6181 gttgtttttg aagatcgttt ttcaccgtat catacaagtt ggctatttcg ttggcaatgt
     6241 cggctgccat ttgcggatcc tcatcaacta ctttgatttc aactgccatg tattctgttc
     6301 tattgaatga aatgttttca ttgtaggttt cgtatagacg ggtgttttta aatttagact
     6361 ccgggtcaat gtcgtaatgt tccagcagat taaatttctg aatgatacgc tgacggattt
     6421 cgtttgaatt gagaacctgc agcatctgtt cggcttcttc ttcttcgcca aatgccatga
     6481 tgtcttcttt cgcgccatac tgactcgaaa tgagggcttt cgatacagca ttggttgatg
     6541 ttgggaaaag aacgaccgat gacacatact tgtttttaat cagcagggat attcctgctg
     6601 aaattactgc agcggcaata caaataatga tgagcagttt tcgccatttg aaaagaaaaa
     6661 acaagaagtt cgtgttttca aatgtcagtt ctttcggagt agtactttct ttcatggtgc
     6721 tgaaaattga cggtgcaaag gtaatgtttc gctgtttatt ttttggggtt tattttttga
     6781 gccgcacaat gatgttggcg caagggaaaa gacgcgataa aaacgatgct tcgtagaatg
     6841 tgcgcatacg ctggctgcag tttataaaag gttcaatcag aaagttaatg aaaaacaccc
     6901 agcttttcga aaaagcaatt tttcggtaaa tggttttttc aaaatgaaac gggaaatagt
     6961 atgatctttc gaatggcgca tgctttgtaa agaaatcaaa ggtctgtatc gaaaacattt
     7021 ttttgtgcgt gggatcgata aaattgaatc gggaactgaa atgtggtaca cgaatttcaa
     7081 taactcctcc tttttttaaa atgcgatgca gttcttcaag cagcggtata tattcgatgt
     7141 gttcgaatat gtcctgacaa agaatataat ccatgctttc gttatcaaaa ggcaatggta
     7201 gcttgttgat gtcgtgtact acatcaacgc cctcgatggg cgatatgtca agatttatcc
     7261 atccttcgcg gatgtcgtgg ccgcagccca gattcagtct tttctccatg caagaaggtc
     7321 gcttatggtg agtttgttcc ttcggaacaa cagtaacgta ttaaccagca gaaatagtat
     7381 ccacacggct gcaataatta tgattccggc tccaggcttc atttgcagaa tatcggtaat
     7441 ccaggcaact ggataaatgg ccagtgcaaa aactgttagc agcaccattg tttttttcaa
     7501 tccggatggt ttcaccagca tcaacgaaag cacaatctgc gaaatggcaa ccagcgattg
     7561 ggtgatcagg gccgaccatg cggctccgcg ttcgaggaac tgaggtatca gaagcagatt
     7621 gagcccgatg ttcagtatca ctccgcaaag ggcaatgatg ttcagctttt tcatgctgcc
     7681 ccaggccgtg agcaatgttc caaaaatata gaccgacgaa accggaataa aagtgaacat
     7741 gagtatttgc agcaccctgg ccgattcggt cacatgctcc ttgtagagcc agttcatgat
     7801 caggtcggca ttgaacagtg aaacagctga taccatcatg gcggaactca gcagcaaaat
     7861 ggccgatagc tgcataaacg gccctacatc ctgttttttg gacatcaggc ggctgaaaaa
     7921 aggaagcaaa atgacagaaa acaaatagga aaacatgccg aatgcatcga ggattcgata
     7981 ggccgaagcg tagattccgg catgcataga accttgtgga agcagccgtt caatcatgac
     8041 cgaatcgaca cggtaataga aaagcatgag aaaggtcaga actgcgaaag gcagactctg
     8101 cctgaaaagt acgatgttca gcttcgtgtt ccattttatt ttcgacagct ttatttttcg
     8161 caagacgata aacaaagcaa tcagaaaagt gatgaggtag gagagggttt gaaatgcagc
     8221 aaaataaaat atgtcgatgc ttatgtcaaa aacatttccc catagcaaaa cagaaaagaa
     8281 aacaatcatc agcagacggt cgagcaccga caaaagactg tcggttttga acatctgcat
     8341 gcctgaaata ttggagcgga ggaacagaat gaacgaagaa agaatctggt ttattccaag
     8401 ccagataagt agtttgaaat gcagggtgtc gtagccgatc agaaaggctg ccacaaagga
     8461 aatgagtgcg taaaaaagag ccagcgctga tttcatgccg agaatgcttc cggtatattt
     8521 tccggccata tgttcatgtt gtgctacact gcggttgttg aagccagtga ggccaaagtc
     8581 gagcatgaat ccgaacagga acgaaaaatt catcacggcg aaatacagcc cgtaatctgc
     8641 cgccccgacc acgttctgga cagtccggtc gatgccgaaa atccagaagg gtttcaccag
     8701 caggttgatg ccaatgagga aaaacaggtt tatgatgaat tttttctgca ttagggaaat
     8761 tgacaaaaaa agcgcaaatt tgcagctaaa ttaatcaaaa tgaatcgtac ggagcataaa
     8821 ccggttattg ccatcaacac ccgccattgg attccgggtc gtatggaggg catcggtcgc
     8881 ttcgaaatga acattgcgac tgagcttagc cgacttcatc ccgaagctga attccattgg
     8941 tttttcgacc gtcgcccaaa gcttgacatg gctgtgcctg aaaacgtgaa aatccatgtt
     9001 ttatttcctc cggccaggca cccaagactt tattacatct ggtatgagtg gtctgtacca
     9061 atgatgctaa agaaaatcaa ggctgatatt tttatttcgg cggacaatat caactccctg
     9121 aaagcgcact gtccaacgat tacagcaatc catgatctga attttgtgca ttatccctac
     9181 gacctgccgc taacatacag ttcgtattac aatacaaaaa cgtcccggtt tattagcaag
     9241 gccatcaagg taattacggt ttcggaattc agccggaatg acataattga gcagtacaaa
     9301 acgccggaga ataaaattca tgttgtttac aatggtgttg ccagccactt tgcgccatta
     9361 aactcatggg tcatttccga aacaagattt aagctgacaa gtggtaaacc atattttttt
     9421 gttttgggat ccatccatcc acgaaaaaat ctggcgaaaa caatcgaggc atacaccctt
     9481 ttcagaaagc aaacgaattg tgattatcca cttgtagtga gtggaaggga gctgtttcta
     9541 aacaaaaacc taggcaatat tctcaacaat aatccatata gaaaagatat tgtttttacc
     9601 ggatatctcg ataataaagc actgggtgaa acactgggct cggcatttgc gctgctctac
     9661 atgtcagtct ttgaaggttt tggtgttcct gtaatcgagg cgtttgcctc ttgtgttccg
     9721 gtggtagctt ccgacgtttc gtccattccg gaagtagccg gtggtgcggc tatgctggct
     9781 aatccgcatg atgccggaga agttgccgat gctatgaaga aactttcaga agatgaacaa
     9841 ctgcgtattt ctctttgcga gaaaggttta aaacgagcga ctgctttttc gtggaaagaa
     9901 agtgcagaaa gattctggaa ggtcattcag gaagtgcttg cagagactaa gattaaatca
     9961 tagatagcag cgcccccaca actttttcag cgccttcata aatctccgaa atccgcgttc
    10021 caagcatgta gcagggtatt gaaaccagtt tgatatttta aacttcgcct ctggtgcacc
    10081 cgtaatattt ttaaatcttt ctgagataat atcacagggc gcattatatt tgtgaaaacc
    10141 cctgacctgc agcgcaggtc aaataataat agcccggacg aagtccgggt aagagacaac
    10201 agcagatacg accctgaatg ggtcgaattc aatataattt ccaacggata aattgagaaa
    10261 aaccatattt tgttttatta ggcccctgca gggccgtttc taaatttttc cattcacatc
    10321 tggcttcgcc ggacgctatt attattcgac ttcttacgga agtcagattt gtcagataaa
    10381 attcagtgca cttttttcaa gaacctgtca tgctgatttt tagctatgac aaacattttt
    10441 atgtctttta tattcaacat gacatttctg acatttgcag aaaacgtgga attatattgc
    10501 tgaaggcaga agatgttctg tttagatcat tttcatcagc gcgtccacga cttttctagc
    10561 gccttcataa atctccgaaa tccgcgttcc aagcatataa catggtgtag tcaccaattt
    10621 gtttttttca tcgattacaa ttcctgtggc gcttgtgatg ctgtgttgcg agctcatctg
    10681 ttcaatatgt gtagcagtat ccttgtcatt gccgatggta acattgcatc cctcaatcac
    10741 tttggccagc agcaccggcg caatgcacag cgccccgatg ggcttgccgg ctgcatgcat
    10801 cgatagaatg gctttttcaa catcggcatt cactttcatt gcggctcctt caaatgcata
    10861 atcgctcaga ttcttggcgg ctccaaaccc tcccggaaac agtaatgcgt cggattctgc
    10921 cacattgaat tctttaatgt ttttgatttt tccacgggca atacgagccg attcaacaag
    10981 cacattgcgt gtttcctgat tttgttttcc ggtcaggtga ttgattacat gatgctgatc
    11041 aatgtccgga gcaaacagct catattgcgc tccctgctga tcgatagcca gcatgctcat
    11101 ggtggtttcg tgaatttcag tgccgtcata caccccgcat cccgataaaa ttatggcaat
    11161 ttttttcatc tgatttttat tgcgaattta ataaaaacaa aaaaacctgc cttgcggaca
    11221 ggttttttaa aagtcttata tcagattatt cttttgtgat ataatgatat tcggttttat
    11281 cacttccatc tacctgctca agccacattt cgtttgattt cagcttgaga atggtgcttt
    11341 cggttgtgtc ggtgtaggta acgcccatga gttcataggt catgtaataa acgagttctt
    11401 ctttgtcgtc tgacagttcc caggtggcat caaaagttgc atttccactg cttgttaccc
    11461 atgtgaaaac agctttgcca tctttcataa attcaatgta atcgtctttg tcgtcagcag
    11521 tcagtgtttg ttcaacacca ttttcatatt cggcatcgag agcccataca ttgataacgc
    11581 gggcttcttt tgatgcaaga ctgatagcag gaccttcttc gtatttgccg caggaagctg
    11641 ctacgaatgc aatggctaca acagccatca ggatttttgt cattgatttc ataggttttg
    11701 ttttttggtt taggttttta tcttaaccag gtccccccgg ttttttttac ttggtgatgt
    11761 aatgatattc gaaagtgttg cttccttcaa cctgttcaag ccagagctcg tccgaggtca
    11821 gtcgcagaat ggtgtattct tcgtctgagg taaacggaat tccgagtaat gaacctgtgt
    11881 aggtaagtct cagatttgct ttgtcagcag tgaattccca ggttccggta taggtggttg
    11941 taaaaccgct cgatacatag gtcacactca gcacgccatc tttttttgct tcaacgtaat
    12001 cgtctttctg atcggcagtc acagtctggg aggcgccgtt cttatagatt tcgtcaattt
    12061 tccatgtgtt gatcaaacgg gcatttttcg atgccagact gaatttcgga ccttcttcat
    12121 acttgccgca tgacactgca acaaatgcaa tcaagactat aagagctgtg attttcgaca
    12181 ttgatttcat attgatttcg gttttgagat agcaaatata aataatattt ttaatatctg
    12241 ttaaaaacca ttttttcctt gttatactat cgatgtaaag gcataaaaca catgtggata
    12301 attatatgtg tttctgatat gctatgtacg gattttactt tggctcaaaa cacctcaaac
    12361 gcaaacctta caatggttgc cgtaactacg atgagaaaga aaatgcggat aaagcggttg
    12421 cctttcagca aagccagctt cgaacccaga tatgcgccgc ccatattgca gatggccatc
    12481 ggaatggcta agcggtaaat aatgttgttg gtcgcagcaa acaaggcaat ggcgctaaga
    12541 ttggtagcca gattcagcat ctttgcattg gcactggctg caatgaaatc gaatcccata
    12601 aagaaaatga aacacaaaat aaaaaacgat ccggttccgg gacctatcat gccatcgtaa
    12661 aaaccaatga tgaagccgaa tagggtgccc gctacaattt gtcttttcaa ttcaacatct
    12721 tttgttttta ccgatccgaa atcttttttc agaaaagtat atactgccac aaccaccaat
    12781 aatgcaagaa tgaccggctt gatgaaatcg tcttcaataa tggtgataca ccaggctccc
    12841 agcgccgatc cggcaaatgc tgctggaaca agaaacagaa aaagtttcgg attgatggtg
    12901 gcttttttcg aaaactgata cgtcgccaaa gctgttcctg aaaaggatgg aatcttggtg
    12961 gtgcccagca atgtggctac gggatattgc ggaaacaaaa acaaaatcgc tggcgtttgt
    13021 atcagcccgc caccaccggc aatcgaatca ataaagccgg caaaaaacga aacaacgcaa
    13081 attatgacga gttgtatttc cacatcaagg attgttggta tataatgctg acttgtgacc
    13141 agtgagcagt gatcggtgat cgtttttcaa ctcatcattc atcacttgtt gtaaaccgct
    13201 catgtcgcgg aaacagaaat attttaagtt gacaaaggca tcgggtggag cagcggcaaa
    13261 gttcgctggt caccgctcat cattcactgc tatttgtgga aggctttcac aaaatcatcc
    13321 acctctttca cgccaccctg caaaacaaga tatccgttat aaggctcgcg ctcggtgatt
    13381 tcatcgttga cgggtgtcag catcatgcgt ttcacttctt ctggatcgtc ggtctgaccc
    13441 agcacccagc ccagcttgat gtaagcggtt tccggcagca tgttggcagc gggaacaatt
    13501 cctttggcca tcaggtcgcg gccggtatcg tatacaaaca tgtggcaata gccatacagc
    13561 gtttgaacag ccatgaacat atgaacacct tttgcatgag cgcgctcaat ggccggatac
    13621 aacggtttgt tgatatgtcc gagaccggtg ccggcaatga tgattccgcg gtagccattg
    13681 tcaaccagac tgtcgataat gtcgggcatc atgtttgggt aataatacac aatggcgacc
    13741 ttctcttcga aatacggata gatattgact ttgcggtctt tgcggcgggg attgtaattt
    13801 tgtttgatgg gaacaactcc cttccgtgtg attttcgcaa gcgggacatc gctcagggtg
    13861 cggaatgtgg accgatatga tgaatgcatt ttgcgcactc gggttccacg gtggaggaat
    13921 ccgtattcat ccgatgttgg tccgaacata caaaccataa cttctgccac atcgccgtga
    13981 ccagcggcgg tcattgcgtg catcagattc agtgctgcat cggagctcgg acggtccgaa
    14041 gagcgctgtg aacctacgag tacaatgggt accggagaat tctgcaccat gaaagtcagt
    14101 gctgctgcgg tatgcgaaag ggtgtcggtt ccgtgtccga taacaattcc atcaatccct
    14161 ttttcaattt cttcgccgat ggcgatggcc agttttttat attgctcagg ccccatgttt
    14221 tcgctgaaaa ccgcaaagag cttatcggtg gtgaggttgc agatgtcggc cagttcggga
    14281 acagcaccat acaattcacc tggtgtgaat gcgggaatca cagcgccggt gcggtaatcg
    14341 agacgcgaag caatggtgcc gccggtgccg agcagtttca cgttgggcag gcccttggtg
    14401 accggaaatt gtttttcagg aattttgtaa ttggcttttt tgtagccggt ttccttcatg
    14461 ttcaatatgg tggaggtgtc aacacccacg ttgtaaccgg tgataatttt cagcacgatg
    14521 tgtttgtcat catcgttttc ggcgcgtggc agcacggtgc cctgaaagtt accgcgggtg
    14581 gtttcaactt cggcctgacc ccatacgcgg acctgatatt tcttcagcag ttcgagcgca
    14641 tggcccttat atccctggaa aaaatcttcg ctcattattt tgaaattttc tgtttaacga
    14701 ttgtatgtaa ttcggtcaga ctcatgttgc cgcgggctat gcgctgcaat tcacccatga
    14761 tccagttgat gcggttttca ggagtgtcgg tgtgacaaac cgtaccaaat ttttcattca
    14821 ggaaatttat tttttccgtg atttcatcag ctttcaacaa tttgaaattc aagctggtca
    14881 gcaccgaatc gaaatccatt ttcggatatt gataaaccac aggcagcatg ttgtatgcat
    14941 aacgcagatc catgtgattt ttcttcaggt actggatcat tttttcaatg ttgctgatgc
    15001 tgaatcccgg acctttttca aaatgtcctt ccacccattt cagacgatgt ccgatgaaat
    15061 ttccaaccca tttcggatcg gtgtcggtat tcttgctgat ggcatcaatg gccggataca
    15121 ggttgttggt gaaaaggtaa taatacgtgc tttcggctac gccccatttc ttcagctgat
    15181 tgtaacgttc actgacttcg ttcggcaatc ctttgcggag ttcttcgata tgctggtctg
    15241 taagtggaat cggagcactg tcggtatcag gatacatgcg gtcggcgcca ggaagcacgc
    15301 gttcgaaaat ggtagagccg tcttcgaagc tttttctggt ttcattcgga atgccttcaa
    15361 atgccatccg gcagcgttct tcaatgactt ccagtgctgt ttttatatct gcttccggtc
    15421 cccagaaaac aatctgtgca tcatcgtctt ttgcattcag catcggagca atgatttcga
    15481 gatccaacgg agtaatgatc ggtttgaatt gctcggtatg aagcatgttt ggtttttcaa
    15541 gacaagcaat tactttaagg cgatccgaaa tttcgttggc gaacattttt gttggctgtg
    15601 tgaaatgact cagaatgcca cggaagcccg ggagattgac ggccacgatt ttgtgtcccg
    15661 catcacgtgc gactttcaat ggctcgtatg ttatctggaa catgtcataa tccagaaatt
    15721 catgacgcat actccagttc gaagctgatc cgaatttctt cagcagttta tcacgcactt
    15781 tcagcagcgc ccactgacgg aaacattcga catgggttaa ttcgggaatc catttagtgt
    15841 ggcttacgcc tttgatttcg atgcgggttc cgcctttaca gctgacgtta acgtcttcgc
    15901 ggccggcgcc gatacctgtg cggaccagtc ctgtactgcg attcagaaaa cgaatgtaat
    15961 cacagacttc tttcacttca tccggatttt tcatgtcggg ataagtgaca gtctcaatca
    16021 acggcattcc aagacggtct gttttataca cgcgggtatg accgatatcg ctgatttcac
    16081 ggcagctgtc ctcttcaatg ctgagctgaa tcagacggat tttcttgtct ttcatcggaa
    16141 gctctccttc gacaccaatg atggcagtgc gctgaaagcc ggttgggatg ctgccgtcga
    16201 gatactgctt gcgtgtgatg tgaacctcac ccacaatgtt cagtttcgac agaagggaaa
    16261 tttttatggc aatatccaat gcctgacgat tgatgggaaa cggcggagtg tcgtccactt
    16321 catacgtgca ggtggtttcg ttttttacac gatacacaat ttccttgcgg gttttgaatt
    16381 ccatcagtgc ggtgccgtcg tattcaccaa gctcgctgag cgttgggcgc atgtgacgga
    16441 tcacttctgc atcgtagatg tagctgggat tgaatttgcc ggccggacaa ttacagaaga
    16501 gctttttttc tgtcagcaat tgctgatgca cttcaagtcc cgacataaat ccgatggagt
    16561 cgtaagtggc ttgtgtggct tcgcggcgcg gaacataccc gacttccttt ttggtattct
    16621 cgtaattggc acgagcatcg aaatcagttc tcatgtgatg gatactgttt ttattctgaa
    16681 ttatttgtca aaagtaaaat aaatcagaaa caaacagcta aaaattgctg gaaaatttca
    16741 aacattggtt gaaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaatc
    16861 agaaacaaac agctaaaaat tgctggaaaa tttcaaacat tggttgaaaa ccccttatga
    16921 atattgaaca ccgaacaagg aagtgtgaat ttcgaagtaa ataccaaatt ctgaatattg
    16981 aacaccgaac aatccgtaca tccttacgga tcaagaggaa gtgtgaattt cgaagtaaat
    17041 tccaaattct gaatatcgaa tacgaaacaa ggaagcgtaa atttcgaagt aaatagttcg
    17101 aaattcgttc ttgccttctt ccttccttgt tctttttaat ccgtaagaat gcacggattg
    17161 ttcagagttc agtgttcgat gttcacttga ccattccgcc gcatatggct tcatcgcatg
    17221 gattcaccat caacagcgtt ccatcggcgt tttcggcaaa gagaatcatt ccgttcgatt
    17281 caacgccttt gatgtcgcgc tttccgagat tggccaccac aaccactttc tttccgatca
    17341 actgctccgg tgtaaaatat tctgctacac ccgaaacaat ggtgcgtgtt tcaaaaccaa
    17401 gatcaacact cagtttcatg agctttttgg ttttggcaac tttttccgct tccaggattt
    17461 ttccgacacg caaatcgagt ttagagaagt catcaaaact cacatcggat ttcaccggct
    17521 caaaacttac attggctgct ttgttggcaa tcttcgatgc ttccagcttg tcgagctgtt
    17581 tctgaatgtc ggcatcttca attttttcaa acaggatttc cggctggttc agctgcaatc
    17641 cttcatccag catgggtttg ccaatattgt tccatgacgt ttcattcatg ttcagcatgg
    17701 ctttcagttt tgtagcagtg aatggcagga atatttccga aagtacagcc atgttggccg
    17761 aaatctgcaa aacgttattc agaatacctt ttacagcttc cggattttct ttatatattt
    17821 tccagggctc cttatcggtg aggtatttat ttcccagacg agcaatatcc ataaagaaag
    17881 caagcgcttc gcggaagcgg aattgctcga gacttccggt aattttttct ttcagcgcag
    17941 acacttcttt cataatgtca tcgagctctg cgccttgtgt tgaagcaggt acacgtcctt
    18001 cgaaatattt atgagtgagc accatggcac ggttgacgaa attgccgaaa atggctgcca
    18061 gttcattgtt gttgcgtgcc tgaaaatcct tccaagtgaa atcattgtct ttggtctcag
    18121 gtgcattggc ggtaagcaca tagcgcaata catcctgctt tccgggaaag tcttcgaggt
    18181 actcatgaag ccacaccgcc cagttgcgcg aggtgctgat cttgtctcct tcgagattca
    18241 ggaattcatt ggcgggtaca ttctccggaa gaatatagct gccttcggca tgaagcatgg
    18301 ccgggaaaat gatgcaatga aaaacgatgt tgtcttttcc gatgaaatgg atcaatcgcg
    18361 aatcatcgct tttccagtat tcttcccagc tgcggccggt ctgctgcgac cagtcgcgcg
    18421 aagcggaaat atatccgata ggcgcatcaa accatacgta cagaactttt ccttcggaat
    18481 ctgccagcgg aacttttacg ccccagtcca gatcgcgggt gacggcacgt ggctgcaatc
    18541 cctgatcaat ccagcttttg cactgaccat acacattggt cttccattcc ttatgtcctt
    18601 ccagaatcca ctcgcggagc cagttttcgt actgatcgag cggcaggaac caatgcgatg
    18661 ttttgcgttt tacgggtgca ctgcctgaca gcgttgattt cggatcaatc agatccgtgg
    18721 cgttgagcga tgttccgcat ttttcgcact gatcgccata ggcgcgttcg ttgccgcaat
    18781 gcgggcaggt gcctgtgatg tagcggtcgg ccagaaattg ttttgcttct tcatcgtaat
    18841 actgctccga ttccatctgt gtgagtttgc cctctgcatg cagctttgag aagaatgcgg
    18901 atgctgtttc gtgatgaacc ggaagactgg tgcgcgaata cacatcgaag ctcattccga
    18961 aatcttcgaa tgattttttg attattccgt gatattcatc caccacctgc tgcggtgtaa
    19021 tgcctttctg cctcgccttg atggtgatgg gaacaccatg ttcgtcgctg ccgcctatga
    19081 acagaacgtc gcgtcctgtt tttcgcagaa agcggacata aatatcggca ggaacataaa
    19141 cgccggccat gtgtccgatg tgtatcggtc cgttggcata aggcaatgca gttgtaactg
    19201 tgtatcgttt gtagtctttc atgggtcaat taagaaaagg gctgtcaacc cttcgactcc
    19261 gctcaggatg acagctgtgt ttaaaaatca ataccggatt gtcaccggca cacttgtatg
    19321 ttttctaaca aattctccga tccggtccat ctcttcgcgg ctcatttttt caagttgttt
    19381 ttcgggtgtc ggccggtcaa tcggatacaa catccattcg atggcgttga ttttcgaaac
    19441 gaatgcagcc agcttcagga gcgattcttc ggtggtattg tcaatttttt cgccttcgct
    19501 ttcacctcga agcatcattg tctgaatgat tttttcgccc ttgaacaact caatgaaatg
    19561 catcacatca ctcaactcaa tgccttttgc agcgcggttt atcaattcaa actgcttcgg
    19621 atccgtactg tcaagtttaa gcaatccttt atcagccagt gcaacggcat cggctacttt
    19681 ctgcttgtgc agctgtgttg agttggtaag caccgtgatt tttgctgcag gggcatactg
    19741 atttctcagc cgcaccgact cctgcatgat gaaatcaaat tcaggatgca tggttggttc
    19801 accgttgccg gcatatgtga tggcatccgg aagttcgccc gcagcctgca tttcctgaag
    19861 tttcttttca agctctgcaa tcacctctgt tgcttttggc aatccgtcgg ccgatgtttt
    19921 gtctgtccat ccgcattcgc agtaaacgca gtcgaatgtg cagatttttt tattggcagg
    19981 cagcagattg attccaagcg acagtcccaa tcgacggctg cgcaccggtc cgaaaatgat
    20041 gcttccgaaa agatgagtat ccatttattt ttcttctttg gacagcttgc cgaaactttc
    20101 gtatgctgca tccagagcgt cttcgaaaga ggcaaatttt tcattcacca ctttgcggaa
    20161 tgctttcgaa cgggcaatac ggccattttt gcgttcttct ttaggcagac gttcgaattt
    20221 cttttcggca tgctgaagag tgtcaatcac ctgatttctt ttttcggcga ctttctccag
    20281 ttccttcatg atcagttctc cgtctttttc gttgtataac gacatgagca tgattccgtc
    20341 aaccaatact tcgttggtca gataattcac gtcttttttg attcctcttt tgtttgccat
    20401 ggcttatttt gtttgatttg tttttgaaaa aatttctttg atggcggaaa aaattccgtc
    20461 tttgtcaaag ccgcattcct tcttgagttc ggcaattgtt ccatgctcga tgaaacgatc
    20521 gggaacaccc agttttatga aaggcaggta atacgaatgc tccatcataa actcggcaac
    20581 cgctgttccc aggccgcctt tcactgttcc gtcctcaatg gtgatgattg ctttgtgatt
    20641 tttgcatgca tgatgcaacg catcttcatc caacggtttc agataaatca tgttgtaatg
    20701 cgaaaccgaa attcctgctt cagaaagctt tgaatacagc gatatgacat cgtttccggg
    20761 ttgtccgatg gtgagaatgg ccacatcatt tccgctgcac agtttttcgg atttgccgat
    20821 ttgtaattca acaaaggatt gtttccagtc ttttacttct gaatttccgc gcgggtagcg
    20881 gattgcaaaa ggtccgtgct tctcagactg agctgtaaac atcagatttc tgagttggat
    20941 tgcgttgatt ggtgatgcaa tgattatatt agggatggct cgaagatagg ccaggtcata
    21001 aataccatga tgtgttgcgc catcttctcc taccagtccg ccacgatcaa tgccgaaaat
    21061 aaccggcaga ttttgaatgg caacatcgtg tatcagctga tcgtatgcgc gctgcaggaa
    21121 ggtcgaataa atagtgcaaa agggaatgcg ctccgatttt gccaatccgg ccgcaaaagt
    21181 aacagcatgt tgttccgaga tgccgacatc gaaaacgcgt tccgggaaac ggttttgcat
    21241 gatgttgagc gaactgcctg tgatcattgc aggtgtgatg gcgcagatgt tttcattttg
    21301 ttcggcaagt tctgtcaggg ttttgccata aacgacctga tatttgggcg gtacggaatc
    21361 gcatgatgtt tccagaattt cccctgtttt tgcatcgtaa cggcctggag catgaaaggt
    21421 tacctgatct ttttcggcgg gtttgtatcc tttgcctttt ttggtgatta catgaagaag
    21481 ctttggcccg ttgatgtcgc gcaggtcttc aagaacattg atgagttgtt ttatgttgtg
    21541 gccgtccaca ggaccaaagt atcggacatt gaatgcttcg aacagattcg attttttcaa
    21601 ccaggtccat tttagcgctg ttgatgtttt attggcgatt ttctgaacaa aagtgccttt
    21661 gaatatgcgc caaatgcttt gtttaagtct gttgtaggtg tgcgatgctg taatacgtgt
    21721 cagataccgg gtgaaggatc ctgtgttttc gtcaatggca attccgttgt cattcagtat
    21781 aacaagaata ttgtttttca gagctcctat gttgttgagc gcttcaaaag cttcgcctcc
    21841 tgtcatgctg ccatcaccga ttacggcaat gtaatgattg gcgtttatac cgctgtcctg
    21901 ctctccgaca gccattccaa gtgctgcaga aatgcttgtg ctgctatgtc cggtgccgaa
    21961 aatatcgtgt tcgctttcag aaattttcgg aaatccactg atgccatctt tctgacggat
    22021 ggtgtgaaat acatcgcggc ggccagtcag aattttatga atatatgact ggtgcccgac
    22081 atcccagatg atgcggtctt ttggtgaatc aaaaatgtag tgcagggcga tggtgagttc
    22141 tactgcgccc agattggctc ccagatgtcc cggatgccgt gccatgtttt caattaaaaa
    22201 atcacggact tcctgcgata acataataag ctcctgcgga gacaattttt tcaggtcttc
    22261 ggggccgtcg atgcgggata aatatgtgcc ggcttttact accatatttc ggggtgcaaa
    22321 ggtaatgatt taagagatac gatttgtgat ttttgattgt cgatctgttg tgcgttttgg
    22381 ggctttattg ttaagaatat tgactaacgg aaggtttttt gtttaattgt atttgatttt
    22441 ctgatgactg attttctgga ctttggatat aaatccgtta cacgttacag tcgttacacg
    22501 ttacagtcgt ggaatttatc acgctgaagc gtggcgttac tatcattaca ttacagtttt
    22561 cagttcgccg cggcggatca gcaatctgtt gtcagtccat tgctgcgttg gaaccgcatg
    22621 cggcaatcct tatgctgcag gcctctgtcg ccgcctttta attagagact ttgtaaaaat
    22681 tttaacttgc gactttgtca tcgcatcaat ttttcaattt catcagcttc taatggaatt
    22741 tcggtcatta gattaatggg ttcatcatca accagaatgt cgttttcaat gcggataccg
    22801 atgctttctt cctgaatgta aatgccgggt tcgcaggtca gcaccatccc ttttttcagt
    22861 gtcatggttc gcagtcctac atcgtgtaca tcgaggccca tgaaatgcga ggcaccatgc
    22921 atgtaatatt tgaagtagag cggggtggat gtggtattgt tttttacatc atcaaccgaa
    22981 aacagtccga gttcaatcat tttctgttcc agcatcaggg caatttgttt gtttgttttt
    23041 tcgatggtgg tacccgggac aagaattttc ttcgcttcct tcaatacgtc aagaaccgca
    23101 ttgtagcatt gtttctgtcg tggtgtaaat tttccgttta ccggaatggt gcgcgaacag
    23161 tcggctgcat aattgccata tacagcaccg aagtcaagca gcatcagatc tccgtctttc
    23221 agcggacagt cgttggtgat ataatggagt acgcaggcgt tttcgccgcc ggcaacaatg
    23281 gggctgtatg catgtcctga tgcgcctctc cgtataaatt catgtgttat ttctgcttca
    23341 acttcgtatt ccatcatgcc tggttttgtt gttttcaaaa cgcgcgtgaa tgcttcttta
    23401 gtaataccaa tggcctttcc gatcatgtca atttcttcct gtgatttaca cagacgtagt
    23461 tctgtagtca gcggtgtgag ccgttcgtat tgatggagcg gaaaaagatt ttttatttca
    23521 tctatcatac gacggtttgc atcgtgagtg ttggtgctgt atttcagata ttcgtttaaa
    23581 cccatgtaga tgttgcgcga ttgctgcgtc atgtcgcgca gtgcggcttt gaagtctgca
    23641 gcccatttca ctgttttaat gccggaaagt tccgatgcct tttcgcggct caggcgcttg
    23701 ccataccaga tgaccatggt gggatcggct tcctgaataa acaagacttc gcgcagattc
    23761 ggatcggggt ggtgcggaca cagacacaac aacgcatttt cttcatcaat gcctgtgaga
    23821 taataaaagt cggagttctg tcggaaaggg tagtcctgat cgccgctcca gggcatgtgt
    23881 tcgttgcccg ccaacactac aatgctttcg ggttgtagtt tttctatcag attttttctg
    23941 tttcgtttaa agaattcggg tgacattggt ttatagcgca tgattttttt tggctaaaat
    24001 acaaaattct ggcagccgtc agtagtcagt gatcagctgt cagcataatt tgttagtggt
    24061 cgtgcgtctc cgtgacagtg cttctcctca acctcaatca gggcgcgcgt cttctacgcg
    24121 tgaccttaca tcttaaagga gcttcacgta aaacgtgaca ggtcgcacac attgtccctg
    24181 gggcctttgg cccaaggcta acatatttag cgctggaatt tactccgccg aggcggaggc
    24241 gcatccaatt gtgctgcgca gcttttttca tgccttcggc tcctgaatca tttccacatc
    24301 ctctgaagat aaaagatgaa agtaaaaagc tctgtcctga gcttgtcgaa gggacaaaat
    24361 tgattatcta atatttgcac ataggcgcat gagtcgcatc ttacatgagc cccatctttc
    24421 cccatctttt ccagaatcac atatttccac atacgccgaa cctgtcacag cttgccgggt
    24481 tttacgcggt ttcacgtgag gcgtcacatg agcgaaacga cacatcttca cattcattcg
    24541 tatctcgtat ttcttatttc gtatttctta tctcgtattt cgtatttcca atcctccgct
    24601 catccggtaa aaaccgtcat tcgccggaaa gtgtcttttc aggttattcc tggataatac
    24661 ttttgccacg tttaagcttg gggcgggtaa aatgtaaatt ttttcatata ttaatttgtc
    24721 ggctttgcag agaaaggagg ttcggaaaac ggaaaaaaga gcagattggt atattgttgg
    24781 aaaacgaagg aaacaaaaat gactcaaaaa cgtttcctgt gtgtaaaaag tatttatctt
    24841 tgcggcatgg aagaaaaatt gaaatatatt ctggaagaag cgtcaaagca ctttatgcgc
    24901 tatggcatca gaagtatgtc gatggatgac attgcaaggg agttgggtat ttcgaaaaag
    24961 accttgtatc agtattttgc tgataaaaag gaattggttc attccgttat gaaatttcat
    25021 atggattata ccaatgattg ttttcggaac atgaaggaaa cgccgggaaa tgcgatagat
    25081 attttgttgc gggtgagtga gatattgatt gaacagcttg gacaaatgaa tccttcggtg
    25141 aaatacgacc tgagcaaata ttatcctgaa gcttttaaaa cgttgatgga ttacaatcgc
    25201 ggacaggtgc tcgatcatat tgagcgcaat ctgatgcagg gaatcaagga aggactatac
    25261 cgcaaagaaa tgaatacccg cattattgca tatttttatc tgatacgaat ggatcaggtg
    25321 atggccatgg aacccgatga tgaaaaaatg aaaagcatta caacagaagc cattctgaat
    25381 gagcttttcg tttaccatgt cagaggtatt gcgaatgaga agggcattga atatctcgaa
    25441 aacactttga tgaaagattt gaaaaacaaa agaaaataaa aaagtttctt acaaaaccag
    25501 aacgctataa tccgacgaaa gatgaaacaa acaatcattt tactaacgct gtttcttttt
    25561 ttgtgcaatc atggatctgc acaggaaaag ctcagtctga ctcttggaca ggcgcaggaa
    25621 tatgcgcttg atcacaaccg cactttgaaa aatgcatcgc tcgacattca gaaagccgaa
    25681 gccaacaaat ggtcaacgct ttcaaccatg ttgcctcagg taaacgggtc cgtcgattat
    25741 gcaaacatga tgggatatca actcaccatg atgggatttg acattgccat gccgccctat
    25801 gccaccattg gtgtgaccac ttcaatctcg ctgagtgcat cgcaggtgat tggcgtacag
    25861 atgcagaata ttgctgcaaa aatggctgac atcactttga agcaaaccga acagcaggtc
    25921 accaaccagg taaagacatt gtatttctct gcgcttgtga tggaagaaac aactgttttg
    25981 cttgattcaa atcttgccaa tatgaagaaa ctgcttgcgc ataccgagaa ttcagttaaa
    26041 gtaggcatac tcaatcagac cgatgcagat cagcttgcag tgcagattgt aacagttgag
    26101 aacacaatca attctgtcaa acgatcgctc gaaatgatat acaatgcttt gcgcctgcag
    26161 ctgggaatca gcgtggatac agagattgag cttactcagg ccattgacga actcgtgaat
    26221 attgagaaag caaattcatt gctcagtgtt ggattttctt cggaaaacag cttcagttat
    26281 caattgctga aaagcagtgt tgatctttca aaagcacagg tcaacatgaa aaagtgggct
    26341 tatgctccat caatctcggc atatcacagc tacaccaaaa aagaatattt cagcgatgag
    26401 ccaacgatga acatgacgcc cccgaacatc atagggctct ctctgaaggt gccgattttt
    26461 tcgagtgcca gcagatatgc ctcactgaaa gccgctcaga ttgattatga aaaacaactc
    26521 aacacactgg ccgatgccgg agaaggtctt gttatccagc acaggcagct gtgtttcaat
    26581 ctaagcagcg cactcgaaac atacgaaacg cagaagagaa atattgaagt caaccaacgg
    26641 atttttgaag acatttcgcg caaattcgag aatggtatgg cttcgagtct tgaagtcaca
    26701 acggcaggtt cagggctgat tacggcacag agcaattatg tccagacatt gctgaatctt
    26761 gtctcggctc agattgcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    26881 cacagagcaa ttatgtccag acattgctga atcttgtctc ggctcagatt gcacttgaag
    26941 aattactgca aaacggaaat taaaaaatga aaacaataaa aaaacaaaat acaatgaaca
    27001 gattgtttca gacaataatt gttgcaggcc tggctatgtt ggttgtcagt tgcggaacca
    27061 aaggtaaatc agaagagcag gtcgttgtgg aaaacaaggt tgagaaagtt aaagttgaag
    27121 cgatacaaaa acaaacggtc gcgcgtaaac ttgatttatc aacgactctt gaaggttatg
    27181 aaaccatgaa agtttctcct tcgcttaccg gaaatattga gcacatttat gttgaagtgg
    27241 gttcatatgt tggtgccgga caaatgctgg tgcgtatgga ccagaataaa tacaatacgg
    27301 cgaaacttgg ttttacgcaa gccagcattg agtttgaacg tgtaaaggca ttgaaggaaa
    27361 caaacactgt ttcgcagcaa acctacgatc aggccaaact gggatatgat caggccaagg
    27421 aaagtctgaa ttttcttacg gagcacacct ttgttaaagc tcgttttccg ggcgttgttt
    27481 cagcaaagaa ttatgttgat ggagagttgt acagcggagc gccgattctt gttatcactc
    27541 agatcaatca gctcaaagca ttggtttcta ttcctgaaac gtattttccg ttgataaaaa
    27601 caggcatgac aatgaatctg cgatctgata tatataaaga tgaaactttt cctgcttcta
    27661 tcgaaattgt gtatccaacc attgatccgg ccacacatac attccaggca aaactcagga
    27721 ttccgaacgg aacatcaaaa cttcgcccgg gtatgtttgt gcgtactacg cttgaaatgg
    27781 gcgaggtgga agcgttgctg gtgccttatc aggccgtgtt gaagctgacc ggatcgaatg
    27841 aaaggtttat tttcgtcaat gaaaatggtg tcgcaaaacg tgtttctgta tcacttggtc
    27901 aacgctatga tgataaagtg gaaattattt ccgatcaggt gaaagaaggg gatcagcttg
    27961 ttatccttgg tcagaccaaa cttgtggatg gaacaaaact cgaagtgatt aaataattga
    28021 aaaactgaaa tcgtttgcag cccgcttctt attgacaaat tcagataagt gagaagtaaa
    28081 ttgcaaatgc ataaactgaa aaccaatggc tatttacaaa tctgcaatca ataaaccggt
    28141 cacaactatt cttctgtttg tggccatcct ggtagttgga ttgttttcgt tctcacgatt
    28201 acccatcgac cagtttccgg atattgaagc accctatata acagtgatga caacctatcc
    28261 gggagccagc tccagtgagg ttgaaaccaa tgtgaccaag gtgctcgaaa acacattgaa
    28321 ctctgttgac gacctcgatg agcttacctc cacttcgaaa gacaacatgt cgttgattat
    28381 gctgaaactc aagtggggaa ccaatcttga cgaagtcatt aatgatgtgc gttcgtatat
    28441 tgatcttaca aaagattatt taccgacggg ttgttcgaat ccgtttattt ttaaattcag
    28501 caccagcatg atgcctatca tcatgtttgc tgttactgcc gatgaaagtt atgccggtct
    28561 ggataaaatt ctgaacgatc aggtcgtgcc tcagctcaat caggtggatg gaatcggaaa
    28621 catttccatt tcgggctcgc caatccgtta tgtgtatatc gatctcgatc aggagcagat
    28681 ggatgcttat ggaatctcgc ttgaacaggt gggttcggcc attagcagca acaatctgaa
    28741 tgtgtcttcg ggtactgtaa aaatggacaa ggagcaatat gcattgcagg tgcggagtga
    28801 atatgtcgag agtgaagaaa ttgccgatat tgttgtgaaa accacgatgg atggcaggca
    28861 ggtttttgtg cgcgatattg ctcaggtgaa agataccatc aaggatctga cacttgatga
    28921 aaaagtgaat ggtcagaatg gtgtgcggct tgtgattgca aaacaatccg gagccaatac
    28981 tgttcaggtt gcttcggatg tgcagaaaca actcgaaaac attaaatcag cgcttccttc
    29041 cgatattgaa atttcggtga tttacgattc tgccgaaacc atcaaggatt ccatttatag
    29101 tctcgaagaa tccattatgt ttgctcttct ttttgttgtg ttggtggtgc tgtttttcct
    29161 cggccgctgg cgtgcaacac ttattattgc gcttaccatt cccattgctt tggtgggttc
    29221 ttttgtctat ctggcttttg ttgacagttc tctcaatatt atttcattgt cttcgcttac
    29281 tgttgccata ggacttgtgg ttgatgatgc tattgttgtg cttgaaaaca ttattaaaca
    29341 tattgaacgt ggctctacgc cgcgcgaagc cgcagttgct gcaaccaatg aagtttgggt
    29401 gtcggttatt gcaaccacac ttgtaattat tgctgtattt cttccgctta ccatgcttgg
    29461 cggaatggcc ggaatcatgt ttaaggaact tggatggatt gttaccattg ttattgccat
    29521 ctctgccatt gttgccattt ctctcacgcc aatgctcgct tcgcggattc tccggaagaa
    29581 aaaagtagct gttgatagta acggacatat aattgaagaa aaagaaaaat cgttgtggta
    29641 tgatcgttca atcggaagga tgcttcataa gctcgatctg cgatatgcca atattctgcg
    29701 ttggtgtctc aatcataaaa aattaacgat gctgattgtg gttgttttct ttgttgtgtc
    29761 gttgattccc gcactgacag ggcagatcgg caccgacttt atgcagcaga gcgatggagg
    29821 ccggcttaca gttacggcag aactgcagcg tggaacacgt ctggaagaaa ccatgaaaac
    29881 cgcacgcaga ctcgaaagcc gctttatgga aattgcgcct gaaataaaaa ttatctctac
    29941 aaccgttggt tccagcgacg aggccaccat ttcagccatg ttctcgacag ctaccaacaa
    30001 caagatttcg atgacggtgg tgtgtagcga caagagcgaa cgtaaacgaa gtatttttga
    30061 aattgcagaa gtgctgcgtc aggaaatgct tttgtatccc gaactcacaa cattccaggc
    30121 gcaggttgca ggtatgatgg gtggtggaat tgccggcact tcaaccgtta atgttgaaat
    30181 ttacggatat gacttcgatc agacagcggt tatggccgaa acagtaaaga aagccatttc
    30241 ggaaaatgtg aaaggagccc gcgatattac catcagccgc gagcccgatc gtcccgagct
    30301 gaaagtggtt gttgataaac aaaaactggc catgcatggg ctttcttcat cagctgtttc
    30361 aatgtatgtg cgcaacaggg tccttggtat gaatgccggt tttctgaaag aggagggcga
    30421 agagtatgat attgttgtac gcatgaaaga ggaaaaccgc aattcgatca gtgctattct
    30481 taatttgagt attccaacac caagcggtgg aaaaataaaa ctcagcgaga tagccagagt
    30541 tgaagaattc tggactccac agaccatcac ccgcaaaagt cgtcagcgta ttgtaacagt
    30601 tgcagtgaca ccttacaaaa catcgcttgg agagctggct gcaggtgtta aggatgaatt
    30661 gaaaaatatt gaagttccca atggcattac attgaacatt ggtggtgatt atcaggaaca
    30721 acaggaaaca tttgccgaca tgacattgtt tatgttgcta atcattatgc ttgtgttcat
    30781 tgtaatggcg tcgcagttcg agtcattcaa aaagcccttc attattatgg cttcggtgtt
    30841 ttttgctttc tcaggaatca ttcttgcttt gttcattacc ggcacaacac tcgatatgat
    30901 tggtgctctt ggtgccatta tgcttgtagg tattgttgtg aaaaacggca ttgtgcttgt
    30961 tgattatact aatctgatgc gtgatcgagg ttacgaattg aaggaagcta ttgcgctttc
    31021 cgggcagtcg cgacttcgtc ctgtgctcat gaccgctctt acggctattc ttggtatggt
    31081 tccgatggcc ataagtaccg gagccggttc cgaaatgtgg cgcccgatgg gtattgttat
    31141 tatcggaggc cttaccgttt caatgtttct tacactgatt gtagttcctg ttctttatgg
    31201 tattatgtcg cgtcatggtg aacgtgatga aacagagcgc cagcgcaagg agtttatttt
    31261 tatggaaatt tctgacgaaa cctcaacaac ttcaaaaaac taatcgaatg aaagctgtat
    31321 tcattgtttt taaccaatca cacacagagc gtgtagagta tatgctcgat aaactcggca
    31381 tccggggttt tacttttctc gaagatgttc agggacgtgg aaatattgat ggagagccac
    31441 atagaggaac acacacttgg cctgagatga attcggcagt gatcactatt gtgcaggata
    31501 atcaggtgga tgatttactc aagtcggttc ataaactcga tcttcgcaat aaagccgccg
    31561 gtgtgcgtgc ttttacgtgg aatattgagc aaatgatgta ataaagatca tcgaatcatg
    31621 aacattgaac aaggagggat gaaggatgtt ttaaatgaat tatccttcag cgttatcctt
    31681 tccttgttca tgattccttg ttcaaagttc ggtgttcatg attcaaaaat ttattatctt
    31741 tgcatcccct taattaaagg gggttgttag ctcagttggt tcagagcgca tgcttcacac
    31801 gcatgaggtc actggttcga atccagtaca gcccaccagc aaaaccgagt ctttagctca
    31861 gctggtttag agcgcttgct tgacaggcaa gaggtcggca gttcgaacct gccaagactc
    31921 ac
//
LOCUS       DAOPKE010000028        31510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1517_length_31510_cov_11.851248, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000028 DAOPKE010000000
VERSION     DAOPKE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_31510_cov_11.851248"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PKY63_05380"
     CDS             complement(<1..1831)
                     /locus_tag="PKY63_05380"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00082.1"
                     /translation="MKLKKILLLIFWLFGSFSMVEVSAQCTVVASAYPTTVCAGDPVT
                     LSSAGACGYLMYNDFNNGTAGTGWVATTGVDFSNPCNPTADGTIYMWMGSAVPLPRTL
                     TTVAFDVNGACEISFDMKYATQSQSSPCEGIDEYNEGVSLQYSINAGATWVDIVYFQP
                     DGVMLTTNPGTSGTCISSPYVTPFTTWANYTFPIPAAAQTSSTMFRWIQIDYSSLTND
                     HWGLDNIEILCPANVLVDWDHGPTVFDPPQVYPTHDTWYVVEITDTVSGLTAIDSVFV
                     DVVSVPTSTFTVTSPVCSDNFSTITYTGTGSSTATFNWLFSGANVLSGSGAGPYNLQW
                     PTGGTMWVSLEVTDSGCTSPATYDTVIVHQAPVASFAADVHEGCQPLPVQFTDLSDPA
                     GSVWQWNFGDMTTSLVQNPSHTFNAAGLYDVSLIVTTAQGCDDTVTYTNYINVFNQPI
                     ANITATPEITSIVDPEITFSSTSTGVSDWFWNFGDNTTSTDAQSVIHSYNDDGVFSVS
                     LIVTSADGCADTAYTSVQVIAEPTFYNVITPDGNGLNDVFFIENAERIPGNLQVFNRW
                     GKKIWESTGESYMNDFDGENFSDGTYFYIYTYGVEMENEHQGTL"
     gene            complement(1838..2308)
                     /locus_tag="PKY63_05385"
     CDS             complement(1838..2308)
                     /locus_tag="PKY63_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00083.1"
                     /translation="MKHLFRGIITLFVLISMVAVNNLYSQEVEFKRSGNWEFLYEIEG
                     VNFYYKVTACNDEANGFFREHVLLKLENTNDYAIVAEWDVLMYYHGECFNCGEKMTAE
                     HHRNIEIAGNGTAEGRCFSFGDKTLSIFSRFLNYDNPKATLTKFELINKVIRQK"
     gene            complement(2493..3359)
                     /gene="prmC"
                     /locus_tag="PKY63_05390"
     CDS             complement(2493..3359)
                     /gene="prmC"
                     /locus_tag="PKY63_05390"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPF00084.1"
                     /translation="MKPATIREFIAKIGSSLGELYSDAAEGKSVAEYLLASLLHTDRA
                     GLYSIGGKIDDPQMLQSIQSAVNRLMENEPVQYVIGKTWFGGLEIKVAPDVLIPRPET
                     EELAEWVISMHEKEQDQLEIIDVGTGSGCLALLLKSRLPGAKVSGIDISDAALQLASE
                     NAVFHGLEISWLKLDILEAVYCPDAGKKLIVVSNPPYVLDSERETMAERVRLYEPEIA
                     LFVPDSDPLRFYRRIAEIFQHDAAAMYFEINPMMDLQLCEMSKQFGFYSEIRNDFFGR
                     NRMLRFIRLSVS"
     gene            3429..4484
                     /gene="ribD"
                     /locus_tag="PKY63_05395"
     CDS             3429..4484
                     /gene="ribD"
                     /locus_tag="PKY63_05395"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPF00085.1"
                     /translation="MNSDEHYMQRCLQLAENGFGKTEPNPYVGAVIVHQDKIIGEGFH
                     EVFGGPHAEVNAVAAVSDKQMLKDSTLYVNLEPCSHHGKTPPCADMIIRLGIPRIVIA
                     MQDPNSLVAGKGIKKLTDAGIEVKNGILGQEARWLNRRFITFHSKKRPYIILKWAQTK
                     DEFIDIDRSDPYAKKRDNWITGPELKILVHRWRAQEQAILIGYNTLINDNPQLNVREW
                     TGRNPQRILITDTITDEHFNIFNEDMKTIVFNPAQDSITEKAEYIKLDFDENLLNNIL
                     KTLFDKNISSVMIEGGRQILESFINSGLWDEARVLTGNKTFGKGLSAPALNNAKEIEH
                     RSFDKDFMQIYINTSTK"
     gene            4481..5344
                     /locus_tag="PKY63_05400"
     CDS             4481..5344
                     /locus_tag="PKY63_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00086.1"
                     /translation="MIWLILALLTSTLIMVCFKLFPKFGIDTLQAIATNYIVAFIAGY
                     ITTTPESATFYIESDWFLLSIASGILLILVFFVFASSTHKAGIAITSVSSKMSVLIPI
                     TLGFFVFGESMQALKIAGIIISLPAFYFIFKRNEHFHLKSSLLILPILLFAGNGANDS
                     LLKTAQFFYLRNDGDLVSYLTAAFGIAFCIGLSIVLIRSIKNKKRIEFKNILAGILLG
                     LLNWYSTLFFLKGLTVVDVTVFIPVFNAGIVCLGAITGLVFFREKMGEWNVFGLGLAV
                     VAIVLIALGNG"
     gene            5337..5951
                     /locus_tag="PKY63_05405"
     CDS             5337..5951
                     /locus_tag="PKY63_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPF00087.1"
                     /translation="MDDAFLTIQKAAQAIFKDRNSRFIGLVFPVDNETQVKEIQAQLR
                     KEYYDARHHCFAYIIGFEKEFFRANDDGEPSGSAGKPIYNQLLSSGVTNVLAVVVRYF
                     GGTKLGVPGLINAYKTATKLALDEAGIVEKHTARTLKLNFTYPEINTVMQIVQTDGVK
                     IKNQLFDNNCSMELLVRESIINAVSSKLEKAGIFCEISSGLMVF"
     gene            6069..7289
                     /locus_tag="PKY63_05410"
     CDS             6069..7289
                     /locus_tag="PKY63_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00088.1"
                     /translation="MSDFTQNIFNYSTNQPMPIKTVFRLFIPIIIVLYSCSSADKPER
                     SSFIWENDYFNNYLSDEDQTRLDSLGITHFYVKYADVQWNLFHEMTEPVYVDDKYGSF
                     NLYNITPVIFITNDVLLFSTPADIKPLAKKIYNLYCYIHQKNAEKLMQSFYENKYDRW
                     TDESSDNFDNWTRYYEAVEDDPDSIVSKWTADNNELLIDCDWTVSTKEKYFSLLKELQ
                     KLDAKIEIQSTLRLWQYRDHELAGVPPVDRCLLMCYSTGNPADPDEENAIVDLKTIKE
                     YINHSGYPKELDIALPVYSWAVLFRNKEFRGIISPVTLTELKEMSFLEQLKDTTRFLV
                     KTDTVIGDLYYRYGDELRFQGVNTKELTDIATWVDKKINPGKNARVSLFSYDTLYFNQ
                     IGNENIDKIFHIFE"
     gene            7252..>7701
                     /locus_tag="PKY63_05415"
     CDS             7252..>7701
                     /locus_tag="PKY63_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00089.1"
                     /translation="MKTLIRYFIFLSSLLILTPAQSKACVYFEYGEDVRISLFRLMND
                     RMLAYSPFVYTSHLFNETISKSHKDLKRNLNEWKSALKADFRDEDMTVVLYKTPPDLF
                     STLYYKNQLSVFFEGNEFVKVLNKPENRDYLWYLVVCKAIEYWELEFS"
     assembly_gap    7702..7801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7802..9660
                     /locus_tag="PKY63_05420"
     CDS             <7802..9660
                     /locus_tag="PKY63_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00090.1"
                     /translation="SDFFTSRYAFQLVRMLFQTNDYKSCVHTYEKHFADHDTASIVNP
                     WALHYCAISQGLTGNSIEANYRKSLAFDQSDDKKFRMYTTFDKSRHVVEQTMLLAKNN
                     HEKANILAMTIMNYPGQALETLNQIAMLDPQNEMLQFLLMREVNKLEDWVLTPELSFS
                     PPSVNMASWDSVTDTYTYVNYMKDRNYMQKVIAFVSSIRSRSAGETAQYYSLMLAHLY
                     TMADNTSAALQLLPQTENERNGHYANQALIEELMILSSQSHFSNIANRNRAGLLMDKM
                     EKSVRAGKMESHTFYSMCKRFSKAFMAMNDLPRCILMLDKADNYQWDFQWYEQYDSLA
                     YSYGYYFYSDIIRHLNQSASSKDMIDFITLTENKNKGAMDIFLTSQRLPGTAQSRELL
                     GSIYFREGRMLDALDTWKKLPQDFWTRYEGFRNYLGKNVFRPKPIYPESNSRLRLRPQ
                     INKYKMLSNILRIQDSALHAVSDRYKYVLQLAHAWYNVSYFGNSWGLAHYENSCNVFE
                     SYEYGFEGIPSRVDNDSSEYHQLKTSKSLYRQVIDGSPDPEQRCEAMAMLQLIAYEEC
                     GTVGFHHFPYADEKTYVPIFTKAMWKKYENTRMYKRLNKRCSYFRDFLKKAK"
     gene            complement(9676..14070)
                     /locus_tag="PKY63_05425"
     CDS             complement(9676..14070)
                     /locus_tag="PKY63_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00091.1"
                     /translation="MKKFLFTVIVFCNVLLLQAQTPVANFVAITPTSGCGFLSVSFQD
                     QSTGNPTSWDWDFGDGTTHSALQNPTHVYSSNGTYTVKLKVTNANGSNTFTRTNYIVV
                     HKKMVADLSAFPVSGCQPLTVQFNDMTALGDGPVASWNWNFGDGFSSVSGNPSHTYTN
                     PGTYNIILLVTDIYGCSSNQTMNSYVTVYPNPVAGFTSTNNISCSSPASVDFNNTSTG
                     SGLSYFWDFGDGNTSTDQNPSHQYNTGQYSVTLIAINANGCSDTLVMNNFVNIVVPVP
                     DISFGPDSGCEPIPVNFQCLNTGIASALWDFGDGGTSTVLNPSHTYTNSGIYTVSFSA
                     VDSYGCPMDTQFTDTVVVFPTPVFDFSSPDPVGCMVPFTVDFMADPGYVNYVWSFGGP
                     DSANASNTYNAFGNYSVTLTVTDINGCVGTLTKPNYVQIAPPPITFSADILSGCVPLD
                     VQFTSSSGGAGTTYIWDYGDGSPLDTAVNPLHTYGDSGVFNVTLSIINTEGCTNTITM
                     SNYIMAGLHPQVGFFAEDTIVCYSEMVAFHDSSSAFVESWNWDFGDGQFGSGPDPTHQ
                     YGDTGTFTVSLVATHNGCRDTLQKEDYIVIWPPIAAFSPSPSLLCSYPDTVHFTDQSV
                     GAETWLWTFDDGGTDTVQNPDYAYAAPGTYMVELLVTNSNGCRDSIEVQVRVDEVFAN
                     FQSNIPEVCMYDPVVFSDLSTAHVAITNRAWTFGDGATSSGAPVSVSHSYNTAGVYDV
                     TLLATDSWGCTDDTTITSVIIVNQLPSPNFTPTTPLTGCTPLTVEFTDNSSAAAPAVL
                     TDWLWNFGNGSTSTAHPTVQYTYYTAGNYNLSLTVTDSKGCDSTRIFYNYIQPTKPNP
                     SFHTTHANNVFCYSETVTFVNTSTAAISYEWDFGDGSPASSLTNPVHHYLVDTTTVFT
                     VTLVATDANGCDSTYSLDITVSIPFPDFTAPVVSTLCPPLVVDFSNTSSSDVIQYLWN
                     FGDSASGTSNTSTTANPQHVYYDPGDYDISLSVTNNFGCVVDTVFPLYVHVGGPTGEF
                     TYAPLSGCAPLMVTFTTTNLNADSVRMVYGDGFSDFDAGSITYTHIYQTGGDFVPTMI
                     LYNYDDTSGVMTCQIMKQDTTNPIHVISGIPAFVASTPVCPDMPLQFIDQSVVYGSVT
                     SWNWDMGNGSASTLQNPTTTYDTPGFYDVSLSVTVDGICSYDTLVHMYVEVLATPDVP
                     IYYSHTAACGPVTTDFAVETDSLIYPAPVFFWNFGNGLTSTDSLHTSTVFNTTGIYDV
                     ALTVTYSNGCTMTDSVSVQIYANTIPEVSCNPQPNPVLAEVDVTLFNTTNTFGIPVEQ
                     LVWSWDLGDGNISDQYQPIHSYSIEGLYDIVLMAFTDSMCSDTAHCPLVVGELLNIPN
                     VFTPNGDGFNDYFEIVYYGNLTMFDLSIFNRWGLEVFHSTDKDFMWDGTINGSEAASG
                     TYFYIVKITTESGKSFEQNGTCTLLREN"
     gene            14428..14625
                     /gene="rpsU"
                     /locus_tag="PKY63_05430"
     CDS             14428..14625
                     /gene="rpsU"
                     /locus_tag="PKY63_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPF00092.1"
                     /translation="MIIVTLKEGDTIDKALKKLKRKFEKTGVARELRDRQKFTKPSER
                     KREVQSKALYVQRMISKQMNG"
     gene            14692..15576
                     /locus_tag="PKY63_05435"
     CDS             14692..15576
                     /locus_tag="PKY63_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPF00093.1"
                     /translation="MIERFITYLTKEKRYSLHTAISYRADLNSFRQYLDKNYDGVAFE
                     KCTPVIIRSWLAEMASMNYNPRSLRRKRTALSSFYRYLIYIGLTETNPTRGIPLPKLP
                     VRLPVFADEKSVQTILASGEKNNDYPTVRNQLIFEIFYATGMRLSELIGIKNTDIDYG
                     KCTIKILGKRNKERIVPFSKQLLEHMNAYESLKKEWFDDLETDSNYFLTNTGRKLYPK
                     YVYRAIHQSLENVTSLSKKSPHVMRHTFATHMLQNGADLNSIKEMLGHANLAATQIYT
                     HTNIKQLKEIYHKSHPKS"
     gene            15595..15891
                     /gene="raiA"
                     /locus_tag="PKY63_05440"
     CDS             15595..15891
                     /gene="raiA"
                     /locus_tag="PKY63_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPF00094.1"
                     /translation="MKISISSVKFKADKKLEDFIQDKIGKLATNYDNLISGEVTLRLD
                     NSANQENKIAEIRLNIPGNDLFAKKQCKTFEEATDSALDALRKQLSKHKEKIHP"
     gene            16030..16102
                     /locus_tag="PKY63_05445"
     tRNA            16030..16102
                     /locus_tag="PKY63_05445"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16063..16065,aa:Thr,seq:tgt)
     gene            16127..16207
                     /locus_tag="PKY63_05450"
     tRNA            16127..16207
                     /locus_tag="PKY63_05450"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16161..16163,aa:Tyr,seq:gta)
     gene            16218..16290
                     /locus_tag="PKY63_05455"
     tRNA            16218..16290
                     /locus_tag="PKY63_05455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16251..16253,aa:Gly,seq:tcc)
     gene            16299..16370
                     /locus_tag="PKY63_05460"
     tRNA            16299..16370
                     /locus_tag="PKY63_05460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16331..16333,aa:Thr,seq:ggt)
     gene            16416..17603
                     /gene="tuf"
                     /locus_tag="PKY63_05465"
     CDS             16416..17603
                     /gene="tuf"
                     /locus_tag="PKY63_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPF00095.1"
                     /translation="MAKEKFDRSKPHINIGTIGHVDHGKTTLTAAITLNLADKGFAEF
                     RSFDSIDNAPEEKERGITINTSHVEYQTDNRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDLVDDEELLDLVEMDIRDLLSF
                     YGFDGDNTPIIRGSALGGLNKEPKWVEKVMELMEAVDNFIPIPTRAVEKDFLMPVEDV
                     FSITGRGTVATGRIETGVINSGEAVDILGLGAEKLKSVVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKNEIRRGMVIAKPGSIKPHKQFKAEVVILKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEVVLPDGVEMVMPGDNLSITVNLITEIAMNQNLRFAIREGGRTVGAGQVTE
                     ILD"
     gene            17662..17734
                     /locus_tag="PKY63_05470"
     tRNA            17662..17734
                     /locus_tag="PKY63_05470"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17695..17697,aa:Trp,seq:cca)
     gene            17762..17953
                     /gene="secE"
                     /locus_tag="PKY63_05475"
     CDS             17762..17953
                     /gene="secE"
                     /locus_tag="PKY63_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPF00096.1"
                     /translation="MRKIINSLREVRDEMLNKVSWPTWLELTESAIVVSIASLIIAGV
                     VLVMDISFENILKLVYGLF"
     gene            17966..18511
                     /gene="nusG"
                     /locus_tag="PKY63_05480"
     CDS             17966..18511
                     /gene="nusG"
                     /locus_tag="PKY63_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPF00097.1"
                     /translation="MSSEESKKWYVVRAVSGSEKKVKQYIDNEIARLGLQDAVAQVLI
                     PMEKVFQIRKGKKVTKERNFFPGYILVEAALDSEIEHIIKNVPGVLGFLGSKGEAIPM
                     RQNEVNRILGKVDELADKGEEMYETFLVGESVRVIDGPFNSFSGVIEEVNEERKKLKV
                     MVKIFGRKTPLELSFMQVEKE"
     gene            18536..18985
                     /gene="rplK"
                     /locus_tag="PKY63_05485"
     CDS             18536..18985
                     /gene="rplK"
                     /locus_tag="PKY63_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPF00098.1"
                     /translation="MAKEVAGLIKLQIKGGAANPSPPVGPALGAKGVNIMEFCKQFNA
                     RTQDQAGKLLPVIITVYKDKSFDFIVKTPPVAVQLLEKAKIQKGSGEPNRDKVASITW
                     EQVQAIAEAKMVDLNCFTVESAMRMVAGTARSMGIKVTGTAPFQTNA"
     gene            19003..19698
                     /gene="rplA"
                     /locus_tag="PKY63_05490"
     CDS             19003..19698
                     /gene="rplA"
                     /locus_tag="PKY63_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPF00099.1"
                     /translation="MARISKKRKEALSKFDKTAQYTLTDALKLVKELTYTKFDSSVDL
                     DVRLGVDPRKANQMVRGTVTLPHGTGKTVRVLVLCGPEKEEEAKAAGADHVGLDDYIA
                     KIKGGWTDVDVVITTPNVMPKVGALGKILGPRGLMPNPKTGTVTMEIGKAVADVKAGK
                     IDFKVDKYGIIHSSVGKSSFTVEKLEDNARELLSTIIKLKPTTTKGTYVKSVFLSSTM
                     SPGIHIDVKSVSN"
     gene            19721..20215
                     /gene="rplJ"
                     /locus_tag="PKY63_05495"
     CDS             19721..20215
                     /gene="rplJ"
                     /locus_tag="PKY63_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPF00100.1"
                     /translation="MKTKEDKNQIVAELTETLKQYDVVYVADILGLNAEQTSSLRRMC
                     FKRDIKLQMVKNTLLKRALENSGRDFSELIPVLKGSSAVMLSDVPKAPAALIQEFRKK
                     TKLPLLKGAYVMEMTVIGDNQLGFLAGIKTKNELVADVVLALKSSAIKVAGALKTIVD
                     KDAA"
     gene            20250..20621
                     /gene="rplL"
                     /locus_tag="PKY63_05500"
     CDS             20250..20621
                     /gene="rplL"
                     /locus_tag="PKY63_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPF00101.1"
                     /translation="MADLKAFAEQLVNLTVKEVTELLQILKDDYGIEPAAATVVAAAG
                     GSADAAAEEKSEFDVILKSAGQAKLAVVKLVKELTSLGLKEAKELVDAAPKAIKEKVG
                     KDEADALKKALEEAGAEVEVK"
     gene            20720..24523
                     /gene="rpoB"
                     /locus_tag="PKY63_05505"
     CDS             20720..24523
                     /gene="rpoB"
                     /locus_tag="PKY63_05505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPF00102.1"
                     /translation="MTTNPKNQRVTFASTSQPDYPDFLEIQVKSFQDFFQLETTPDNR
                     KNEGLYKVFAENFPISDTRNNFVLEFLDYYIDPPRYAIEECIERGLTYSVPLKAKLKL
                     YCTDPEHEDFETITQDVYLGMIPYMTDKGTFVINGAERVVVSQLHRSPGVFFGSSRHP
                     NGSILYSARIIPFKGSWMEFATDIHNVMYAYIDRKKKLPVTTLLRAIGWESDKQILEL
                     FDLAEEIKVSKAAIKKEVGRRLAARVVKAWVEDFVDEDTGEVVSMERTEVILERETII
                     EEKHIDIIANSGVKTVLLHKEDAANADYSVIFNTLQKDTANNAKEAVEYIYRQLRNAE
                     PPDEETARGIIDKLFFSDKRYDLGEVGRYRINRKLGRQKSEDRILTKEDIIDIIKYLV
                     ELINSKAEVDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFKPID
                     LINAKTLSSVINSFFGTNQLSQFMDQTNPLSELTNKRRISALGPGGLSRERAGFEVRD
                     VHYTHYGRLCTIETPEGPNIGLISSLCVYAKINQLGFIETPYRKVTKGIVDLKALPVY
                     LSAEDEEEKIIAQASTPIDEKGHFIEDRIKSRHLADYPIVEKTDLDMMDVAPNQIASI
                     AASLIPFLEHDDANRALMGSNMMRQAVPLLCPEAPIVATGLEGKVAQDSRVLINAEGD
                     GVVEKVDARQITIRYHRNDTDKLVSFEDDIKSYHLTKFAKTNQSTTINLKPIVKKGQK
                     VHKGQVLCEGYATDNGELALGRNLLVAFMPWKGYNFEDAIVISERVVREDVFTSIHIE
                     EFIMEVRDTKRGIEELTSDIPNVSLEAIKNLDANGLIRVGAEVKEGDILIGKITPKGE
                     TDPTPEEKLLRAIFGDKAGDVKDASLKAPPGVSGVVIDKKLFSRSIKDKKAKTKEKST
                     LDTLENEFEHQVSEIRKRLIEKILVITEGQVCQGVSNNLRQEVISKGTKFTAKALNNI
                     DFINIIPTNWTKDAKANELIQRLLHNFEIKYKEVLGVYQRKKFVTQVGDELPTGIVQL
                     AKVYIAKKRKLRVGDKMAGRHGNKGIVARIVRDEDMPYLEDGSPVDIVLNPLGVPSRM
                     NLGQIYETILAWAGQKLGTKFATPIFDGASVDQINGYLEKAGLPKNGKTYLRDGGTGV
                     PFDQPATVGVIYMLKLHHLVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAFGAAHILREVLTIKSDDVMGRAKSYESIVKGEPMPKPGIPESFNVLIHELKGLGL
                     NVTFE"
     gene            24556..28842
                     /gene="rpoC"
                     /locus_tag="PKY63_05510"
     CDS             24556..28842
                     /gene="rpoC"
                     /locus_tag="PKY63_05510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPF00103.1"
                     /translation="MAFRRENAPKSNFSSIIISLASPEKILEMSFGEVTKPETVNYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LTVPVAHIWFFRSLPNKIGLLLGLPSKKLEQIIYYEKYVIVNPGIKAADGFKQNDFLT
                     EEEYFQIMETLPRENQMLDDSDPNKFIAKMGGEALFDLLSKIDLDELSPALRNKANNE
                     TSQQRKTDILKRLQVVESFRDGNKRIENRPEWMIIKVVPVIPPELRPLVPLDGGRFAS
                     SDLNDLYRRVIIRNNRLKRLIEIKAPDVILRNEKRMLQEAVDSLFDNSRKVNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLSECGLPKEMASELFK
                     PFIIRKMLERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVMLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLSNEAILEAQLLMLSPHNILNPA
                     NGAPIAVPSQDMVLGLYYMTKGRKSSKEHVVKGEGITFYSPEEVIIAHNEQKADLHAM
                     IKVRLTDSEGNASIIETTVGRVLFNRVVPEKIGFINTLLTKKALRDIIGEVLKNTNNV
                     EAAKFLDDIKSLGFQMAFKGGLSFNLGDIIVPAEKEIFINEAKNQVDEVMGNYNMGFI
                     TNNERYNQIIDIWTHTNSKLTKVLIDKLSKDRDGFNSVYMMLDSGARGSKEQIRQLSG
                     MRGLMAKPQKAGAAGGEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAG
                     YLTRRLVDVAQDVIINEPDCGTLRGLTVTALKKNEEIVESLFDRILGRVTLHDVFNPI
                     TGELIIEEGQEITEEYAKAIEEANIEEIEIRSVLTCESKRGVCAKCYGRNLATGRMVQ
                     KGEAVGVIAAQSIGEPGTQLTLRTFHIGGAASNVSIASKLEAKYDGLLEIDELRCIDT
                     KSEDGKTVKVVMGRSAEMRIRHPKTQAILQSANIPYGSQLFTENGQQITKGDLICEWD
                     PYNAVILSEVSGKIMFEDVLENITFREESDEQTGFHDKVIIESRQKARTPAIKLVSST
                     KEELKRYDLPVGAHITIEEGTKVNAGDVIAKIPRTLGKAGDITGGLPRVTELFEARNP
                     SDPAVVAEIDGIVSFGTKLKRGNKEIIITSKTGEEKHYLINTTKQILAQENDFIKAGT
                     PLSDGAISPGDILAIKGPTKVQEYLVNEIQEVYRLQGVKINDKHFETIVRQMMRKVEI
                     LDPGDTRFLEGELVNKIEFQEENDAVFNKKYIESAGDSEVLKAGSIVSARKLRDENSM
                     LKRKDKVLAEARDAQPATANQILQGITRAALQTESLLSAASFQETTKVLTEAAVNAKR
                     DTLLGLKENVIVGHLIPAGTGLREYDDLVVGSMEEYQSLLASKEETLEKV"
     gene            28844..29155
                     /locus_tag="PKY63_05515"
     CDS             28844..29155
                     /locus_tag="PKY63_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPF00104.1"
                     /translation="MDDKKKQDNSLNIELTADVAEGSYANLAIISHSSAEFILDFVSI
                     MPGMPKAKVKSRIIMTPNHAKRLLKALAENVSKYEKTFGEITEHEQVPPYQMFTPAGE
                     A"
     regulatory      29170..29230
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            29264..30448
                     /locus_tag="PKY63_05520"
     CDS             29264..30448
                     /locus_tag="PKY63_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPF00105.1"
                     /translation="MLNSIAEIIIAGMIASWLCRLIRIPDLIGFLLIGLLFGPYVLQI
                     LDPSFTALSGELRTAALIVILLRAGFELKKDTLKKVGRPVLLLALFPPLFETLAAATI
                     CVYAAGLSWNEGLMLGIILSAVSPAAVVPLMIRFRNEKRGINKEIPTMVLAASSLNNI
                     LVLIVFSILVVFLVRDEILTASAITSVPISIATGITIGIVSALLLIKIFDRFNPRATR
                     RVLLILAFAVVFLRLEKEFAQWVPFAALLAVMTLGYILLNKREKYAHEISQKLAKIWI
                     LAEMILFTLVGAEVNPEIAIKIGLTGLLIIGGGLLVRSLVTWLCLTGTNLNKREKGFV
                     VISYIPKATVQAAIGAVPLALTHTAGAEIILAVAVLSIVVTAPLGAIAIEISGKRWLE
                     KA"
     gene            complement(30961..31031)
                     /locus_tag="PKY63_05525"
     tRNA            complement(30961..31031)
                     /locus_tag="PKY63_05525"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30997..30999),aa:Cys,seq:gca)
     gene            31117..>31510
                     /locus_tag="PKY63_05530"
     CDS             31117..>31510
                     /locus_tag="PKY63_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00106.1"
                     /translation="MNKQIHLSAGNHRNVDVVFVSFERDEKLIAALKSMGNVRWSATL
                     RKWYFPKAAFDLSKLKLAMNGLAIIDDTLLKAATHNKPTAQSDVIPSDILTGLAEFKK
                     WMLHKRYSDSTIKTYVEAAKSFLVFIHPK"
BASE COUNT         9752 a         6970 c         6720 g         7968 t
ORIGIN      
        1 ttaaagtacc ctgatgctca ttttccattt caacaccata ggtgtagatg taaaaatatg
       61 tgccgtcaga gaaattttcg ccatcgaaat cattcatata actctcgccg gtactttccc
      121 agattttttt accccaacgg ttgaaaacct gcagatttcc gggaatccgt tcagcattct
      181 caataaaaaa gacatcgttc aagccattac catcgggcgt aatcacatta taaaaagtag
      241 gttctgcaat gacctgaaca gaggtataag ccgtatcggc acaaccatcg gcagaagtaa
      301 caatcagaga aacggaaaac acaccatcat cattataaga atgaataacc gactgcgcgt
      361 ctgtagaagt agtgttgtcg ccaaaattcc aaaaccagtc agacacaccc gtactcgtac
      421 tgctgaacgt aatttcagga tcaacgattg aagtgatttc aggagtagcc gttatattgg
      481 caatcggctg attgaaaaca ttgatgtaat ttgtataggt taccgtatcg tcgcagcctt
      541 gcgcagtagt aacaatcagc gatacatcgt acaatccggc tgcattgaaa gtatgagacg
      601 gattttgcac caacgatgtt gtcatatcgc caaaattcca ttgccagact gatcctgccg
      661 ggtcagacaa atcggtaaac tgaacaggaa gaggttgaca tccttcgtgg acatcggcag
      721 caaacgaagc taccggggcc tgatgaacta tcaccgtatc atacgtagca gggctcgtac
      781 aaccactatc ggttacttca agacttaccc acatagtacc accggtaggc cactgcagat
      841 tgtatggtcc ggctccggaa ccgctgagaa catttgcacc actgaaaagc caattgaatg
      901 tggcagtaga agaacctgtg ccagtatagg taatcgttga gaaattatca gagcaaaccg
      961 gagaagtaac agtaaaggta gaggttggta cagaaaccac atcgacaaaa acgctgtcta
     1021 tagctgttaa accactgact gtatctgtaa tttcaacgac ataccaggtg tcatgcgtag
     1081 gataaacctg aggcggatcg aaaaccgtgg ggccatgatc ccaatcaacc aatacgtttg
     1141 caggacacag aatttcaata ttatcgagtc cccagtgatc gttggtcaaa ctggaataat
     1201 caatctgaat ccatcggaac atagtagaag aggtctgggc agctgcagga atgggaaatg
     1261 tatagttggc ccaggttgta aaaggtgtta cataaggaga agagatacag gttccggaag
     1321 tgccggggtt ggtagtcagc ataacaccat cgggctgaaa ataaacgata tcgacccagg
     1381 tagcacctgc attgatggaa tattgaaggg aaacaccctc gttgtattca tcaattcctt
     1441 cgcatggtga tgattgtgac tgggtagcgt atttcatatc aaaagatatt tcacaggcac
     1501 cgttgacatc aaaagcgaca gtggtcaggg tgcgaggcag aggcacagcg cttcccatcc
     1561 acatgtaaat ggtgccatcg gctgtaggat tgcaaggatt ggagaaatcg acgccagtgg
     1621 ttgcaaccca tccggtaccg gcagttccgt tgttaaagtc attatacatc aaataaccgc
     1681 aagctccggc tgaagaaagc gttaccggat caccagcgca aaccgttgtg ggataagccg
     1741 atgcaaccac agtacattga gcagatactt caaccattga aaaactgcca aacagccaaa
     1801 aaattaataa aaggattttt tttaatttca taatcgctta tttttggcgg attactttgt
     1861 taatcagttc aaattttgtc agtgtcgctt ttgggttgtc ataattcaga aaacggctga
     1921 aaatgctcag cgttttatcc ccaaaagaaa aacaacgacc ttcggctgtt ccgtttccgg
     1981 caatttcaat gttgcgatgg tgttctgcgg tcattttttc accacagtta aagcattcgc
     2041 catgataata catcagcaca tcccattcag caacaatcgc ataatcattc gtgttttcaa
     2101 gtttcaacaa aacatgttcc ctgaaaaacc cattggcttc atcgttgcag gctgtaactt
     2161 tgtaataaaa attcacgcct tcgatttcgt ataaaaactc ccagttaccg ctacgcttga
     2221 attcaacctc ctgtgaatac agattattga cagcaaccat agaaatcaag acaaacaagg
     2281 taattatccc cctaaaaaga tgtttcatgt gaaaaatttt gaattagaca cttttttttt
     2341 ccaaacgttt aaaaagttgt ttgcaaattt aattaaaagg gttttaattt cataaacaaa
     2401 acggtggggt atatcgaaaa atactcaaaa ataacacccg aaaacccgaa aaaaaactca
     2461 acaaaaagtt aatcaagccc gtattaatct gactatgaaa cacttaatct gataaaacgc
     2521 aacatcctat ttcggccgaa aaaatcgttt cgaatctcag aataaaaccc aaactgcttg
     2581 ctcatttcac ataattgtaa atccatcatc ggattaattt cgaaatacat agcagctgca
     2641 tcgtgttgaa agatctctgc tattcgcctg taaaagcgca acggatctga atcaggaaca
     2701 aacaaggcga tttccggctc atacaacctc accctttcag ccattgtttc gcgctcacta
     2761 tcaagtacat aaggcggatt gctgacaaca atgagttttt tcccagcatc aggacaatac
     2821 acagcttcga gaatatccaa tttcaaccag ctgatttcaa gtccatgaaa aacagcattt
     2881 tcagaagcca gctgcagtgc agcatccgaa atatcaatac ccgaaacttt tgccccgggc
     2941 aatctgcttt ttaaaagcaa agctagacaa ccgctgcccg tgccaacatc aataatttcg
     3001 agctgatcct gctctttttc gtgcatacta ataacccact ccgccagctc ttcggtttcg
     3061 ggccgtggaa tgagcacgtc aggcgcaacc tttatttcaa gcccgccaaa ccatgttttt
     3121 ccaatcacat actgcaccgg ttcgttttcc attaatcgat tgactgcaga ctgaatactc
     3181 tgaagcattt gcggatcatc aattttccca ccaatggaat acaagccggc acgatcggta
     3241 tgtaaaagag aggccagcag atattcggcc accgactttc cctcggcagc atcagaatat
     3301 aattcgccga gagatgaacc aattttcgca atgaattcgc ggatagtagc aggtttcata
     3361 aatacaaata taatcaaaaa gacacaatcg cccggcatgg cttgcaaaca ttttcttact
     3421 ttcgcagcat gaattccgat gaacactaca tgcaacgatg cctgcaactg gccgaaaacg
     3481 gttttgggaa aacggagccc aatccgtatg tgggtgctgt cattgtacat caggacaaaa
     3541 taatcggaga aggatttcac gaggtctttg gtggacccca tgccgaagtg aatgcagttg
     3601 ccgctgtatc tgataaacaa atgctgaaag acagtacgct ttatgtcaat ctggagccct
     3661 gctcccatca tggtaaaaca cccccatgtg cagatatgat tatccgactt ggtattcctc
     3721 gcattgttat tgccatgcag gatccaaact cactggttgc cggaaaggga ataaaaaaac
     3781 taactgacgc aggaattgaa gttaaaaacg gaattctcgg acaggaagcc cgctggctga
     3841 accgacgttt tattactttt cattcgaaaa agcgcccata tatcattctg aaatgggcac
     3901 agacaaaaga tgaattcatt gacatagacc gtagtgaccc ttacgcaaaa aaaagagaca
     3961 actggattac aggaccggaa ctgaaaatac ttgtacatcg ctggcgagct caggagcaag
     4021 ccatactgat tggttacaac acactgataa acgacaatcc tcaactgaac gttcgcgaat
     4081 ggaccggaag aaatccgcaa cgaatcctca ttacagacac tatcacagat gaacatttca
     4141 atattttcaa cgaagacatg aagacaattg ttttcaatcc ggcacaggat tcaatcactg
     4201 aaaaagctga atacatcaaa ctggacttcg acgaaaatct tctcaataat attctgaaaa
     4261 cgctttttga caaaaacatc agcagtgtga tgatagaagg cggtcgtcaa atccttgaat
     4321 ccttcattaa cagcgggctt tgggatgagg cacgtgttct gacaggcaac aaaacattcg
     4381 ggaaagggct atctgcgccg gctttgaata atgcaaaaga aattgaacat agaagtttcg
     4441 acaaggattt tatgcagatt tacatcaata cctcaacaaa atgatctggc ttatactggc
     4501 attgctgaca tcaacactga taatggtttg tttcaaactg tttccaaaat tcggtattga
     4561 tacacttcag gccatagcca caaattatat tgtcgcattt attgccggct acattacaac
     4621 aacacccgaa agtgcaacat tttatataga atcagattgg tttttactaa gtattgcatc
     4681 cggcattctt ctgattctgg ttttttttgt ttttgcttca tcaacacaca aagcaggcat
     4741 agccatcaca tcggtaagca gcaaaatgtc agtgttgatc ccaatcacgc ttggattttt
     4801 tgtgtttgga gaatctatgc aggcactaaa aattgcaggc atcatcattt cattacctgc
     4861 attctacttc atttttaaaa ggaatgaaca ttttcatctg aaaagttctc tgcttattct
     4921 acccatactg ttgtttgcag gaaacggggc taatgattcg ctgcttaaaa ctgcacagtt
     4981 tttctatctc cgcaacgatg gcgatctggt aagctatctg acggcagcat ttggaattgc
     5041 tttctgtatc ggactttcca tagttcttat ccgctcaatt aaaaacaaaa aacgaattga
     5101 attcaaaaac atactggccg gcatcctgct cgggctgctg aattggtatt ccaccctgtt
     5161 ttttctgaaa ggcctcactg ttgttgatgt caccgttttc attccagtgt ttaatgccgg
     5221 tattgtatgt ctgggagcga ttaccggact ggtatttttc agggagaaaa tgggtgaatg
     5281 gaatgttttc ggactcggat tggctgtggt agccattgtt ttaatcgcac tgggaaatgg
     5341 atgatgcttt tctgaccata caaaaagccg ctcaggccat atttaaagac cgaaacagcc
     5401 gttttatagg actcgtattt ccggttgaca atgaaaccca ggtgaaggaa attcaagcgc
     5461 agctccgcaa ggaatactac gatgcaaggc atcattgctt tgcatatatc atcggatttg
     5521 aaaaagagtt ttttcgcgcc aacgacgacg gagagccctc cggcagtgcg ggcaaaccca
     5581 tttacaacca attactctcg tcgggggtta ccaatgttct tgcagtagta gtgcggtatt
     5641 tcggaggaac aaagctgggt gtgcctggac tgattaacgc ttataaaacc gcaacaaagc
     5701 tggcgctcga cgaagccggc attgttgaaa aacacaccgc ccgcacttta aaactgaatt
     5761 ttacctaccc cgaaataaat actgtcatgc agatagtgca aaccgatggc gtaaaaatca
     5821 aaaatcagct cttcgacaat aattgctcca tggagctcct ggtgcgtgaa tccattatta
     5881 atgccgtttc atcgaaactt gaaaaggcgg gcattttctg cgagatttca agcgggttga
     5941 tggttttttg aatttataca tctcttgcaa gagagcaatt attgctgact ttgcctcata
     6001 aagacatcgg ctttaaaaaa atatttttaa aatcctgtca aaaccattct tttctttctt
     6061 aattttggat gtcggatttc acccaaaaca tattcaatta ttctacgaat cagcccatgc
     6121 cgataaaaac agtattccgt ttattcatcc cgatcattat tgttctgtat tcgtgttctt
     6181 cagcagacaa gccggagcgg tcatccttta tctgggaaaa tgattatttt aacaactact
     6241 tgagcgatga agatcaaacg cgtctggatt ctctgggcat cacacacttc tatgtgaaat
     6301 acgctgatgt ccaatggaac ctgtttcacg aaatgacaga gccggtgtat gttgatgaca
     6361 aatatggctc attcaatctg tacaacatca ctccggtaat attcatcacc aacgatgtgt
     6421 tgcttttcag cactccggcc gacatcaagc cccttgcaaa aaagatctac aacctgtatt
     6481 gttacattca tcagaaaaat gcagaaaaac tcatgcaaag cttctatgaa aacaagtatg
     6541 acagatggac tgatgaatcc tcggataatt ttgacaactg gaccagatac tacgaagctg
     6601 tggaggacga tcctgattcc attgtttcaa aatggaccgc tgacaacaat gaattgctca
     6661 ttgattgcga ctggacagta tcgaccaaag aaaaatattt cagtttattg aaagagttac
     6721 agaaactgga tgccaaaatt gaaatccaaa gcacgctgcg tttgtggcaa taccgcgatc
     6781 atgaactggc cggcgtacct cccgtagacc gttgtctgct gatgtgctac agcacaggaa
     6841 atccggccga tccggatgaa gaaaatgcca tcgttgatct caaaaccatc aaggaataca
     6901 tcaatcactc cggctatccg aaagagctgg atattgccct gcccgtttat tcgtgggcgg
     6961 tgttgttcag aaacaaagaa ttcagaggta taatcagccc tgtaacactg acggagctga
     7021 aagaaatgtc gtttcttgag caactgaaag acaccacccg attcttggta aaaacagata
     7081 ccgttatcgg cgatttgtat taccgctatg gagacgagct ccgcttccag ggcgtgaaca
     7141 cgaaagagct gaccgacatt gccacatggg tggataaaaa aatcaatccc ggaaaaaacg
     7201 ccagggtttc gcttttttca tatgacacgt tgtattttaa tcagatcgga aatgaaaaca
     7261 ttgataagat atttcatatt tttgagtagt ctgctgattc tgacaccagc gcaatcaaag
     7321 gcctgtgtgt attttgaata tggcgaagat gtgagaataa gtctgttcag acttatgaac
     7381 gaccgcatgt tggcctacag cccatttgtt tatacttcac atttgttcaa tgaaactatt
     7441 tccaaatcac acaaagatct gaagcgaaat ctgaatgaat ggaaatcagc tctgaaagcc
     7501 gactttcgtg atgaggacat gacggttgtc ctatacaaaa ccccacctga tctcttctcc
     7561 acattgtatt ataaaaacca gctgtctgtt ttttttgaag gaaatgaatt tgttaaagtg
     7621 ctgaataaac ctgaaaacag agattatctc tggtatctgg ttgtatgcaa agccatcgaa
     7681 tactgggaac ttgaattcag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 ncatcagatt tttttaccag tagatatgct tttcagctgg tcaggatgct ttttcaaaca
     7861 aacgattaca aaagttgcgt gcatacctat gagaaacatt ttgccgacca tgacactgca
     7921 tcgattgtaa atccatgggc gttgcattac tgcgcaatat ctcagggtct cacaggtaac
     7981 agcattgaag caaattatcg taagagtctg gcatttgacc agagcgatga caagaaattc
     8041 cgcatgtaca ctacatttga caaaagccgg catgtagtgg aacaaacgat gctgcttgcc
     8101 aaaaacaatc atgaaaaggc gaacattctt gccatgacca ttatgaatta tcctggtcag
     8161 gcacttgaaa cgctgaatca gattgcgatg ctggatccac agaatgaaat gctgcaattc
     8221 cttctgatgc gcgaagtgaa caaactcgaa gactgggtgc tgacgccaga attgtctttc
     8281 agtccgcctt cggtaaacat ggccagctgg gacagtgtga cagacacata cacctatgtg
     8341 aattacatga aagaccgcaa ttacatgcag aaagtgattg cttttgtttc atcaattcgc
     8401 agcagatcag ccggcgaaac tgcgcagtat tattcgctca tgctggctca tctatacacg
     8461 atggccgata atacctcagc agcgctgcaa ctgttgccgc aaacagaaaa tgaaagaaac
     8521 gggcattatg caaaccaggc cctcatcgaa gaattaatga ttttgtcgtc gcagagccat
     8581 ttttcaaaca ttgcaaatcg taaccgggcc ggcctgctga tggataaaat ggaaaaatca
     8641 gtacgagctg gaaaaatgga atcgcatacg ttttattcca tgtgcaaacg gttctcaaaa
     8701 gcttttatgg ccatgaacga cctgccgagg tgcattctga tgctggataa agctgataat
     8761 tatcaatggg atttccaatg gtatgaacaa tatgattcat tggcctatag ttacggatat
     8821 tacttctaca gcgatatcat cagacatctg aatcagtctg catcttcgaa ggacatgatt
     8881 gattttatca ccttaactga gaacaaaaac aaaggagcca tggatatatt tctgacgtcg
     8941 cagcgattgc ccggaacagc tcaaagccgc gaactgttag gctccatata tttcagggaa
     9001 ggcagaatgc tggatgcgct tgatacctgg aaaaaactgc ctcaggattt ctggacccga
     9061 tatgaaggat tccgaaatta tctgggtaaa aatgttttca gacccaaacc aatatatcct
     9121 gaaagcaatt ccagactgag actcagacct caaatcaaca aatacaaaat gctgtcaaac
     9181 attttgagaa ttcaggactc cgctctgcat gccgtgtctg atcggtacaa gtatgtgctg
     9241 caacttgcac atgcatggta taatgtttca tacttcggca acagctgggg tctggcccat
     9301 tacgagaatt catgcaatgt gtttgaatca tacgaatatg gattcgaagg gattccttca
     9361 agagtagata atgactcatc agagtatcac caactgaaaa catctaaaag tctgtaccgc
     9421 caggtgattg atggaagccc tgatccggag cagcgttgcg aagctatggc catgttgcag
     9481 ctcatcgcgt atgaggaatg tggaacggtt ggtttccatc acttcccata tgcggatgag
     9541 aagacgtatg tgccgatttt cacaaaagcg atgtggaaaa agtatgagaa caccaggatg
     9601 tacaagcgtc tgaataagcg ctgcagctat tttcgcgact ttctgaaaaa ggcgaagtga
     9661 tgtccaaacc gcgctctaat tctccctcag caaggtgcaa gtgccattct gctcaaagga
     9721 ttttccggat tccgttgtga ttttcacaat atagaaatag gttcctgatg cagcttcgga
     9781 gccattaatg gttccatccc acatgaaatc cttgtctgta ctgtgaaaaa cttcgagccc
     9841 ccaccggttg aatattgaaa gatcaaacat agtcagattg ccataataaa caatttcgaa
     9901 ataatcatta aaaccatcac cattgggcgt aaatacattt ggaatattca aaagctcccc
     9961 taccaccagc ggacaatgag ctgtatcgga acacatgctg tcggtgaatg ccatcaacac
    10021 aatgtcatac aatccttcga tactgtaaga atgaatcggc tgatattgat ccgaaatgtt
    10081 gccatcacca aggtcccacg accaaaccaa ttgttcaaca ggaattccga aagtgtttgt
    10141 cgtattgaaa agcgtaacat ccacctcagc cagaacaggg ttaggctgcg gattacacga
    10201 cacttcagga atagtgttgg cgtatatctg aacggaaaca gaatcagtca tcgtgcaacc
    10261 attagaatat gttacggtta acgcaacatc gtaaatacca gtagtattga aaacagtgga
    10321 tgtatgaaga gaatcagttg aggttaaacc attcccgaaa ttccagaaaa atacaggagc
    10381 aggataaatc aaggaatcag tttcgacagc aaaatcagta gttacaggtc cacatgctgc
    10441 agtatgacta taataaatcg gaacatccgg cgtcgccaaa acctcaacat acatatgaac
    10501 taatgtatca taactacaga ttccgtcaac agtaacagaa agcgagacat cgtaaaatcc
    10561 aggagtatca taggtcgttg tagggttttg caacgtgctt gcagagccat ttcccatatc
    10621 ccagttccac gaagtaaccg aaccatatac aacagactga tcaataaact gcaaaggcat
    10681 atccggacaa accggggtgc tggcaacaaa cgcaggaata ccagaaataa catgaatcgg
    10741 attggtggta tcctgcttca taatctgaca ggtcataaca ccagaagtat catcataatt
    10801 atacaaaatc attgttggca caaagtcacc acccgtttga taaatatgtg tgtaagtaat
    10861 gcttcctgca tcaaaatcgc tgaacccatc cccataaacc attctcacag aatccgcatt
    10921 cagattggta gttgtgaagg taaccatcaa aggcgcgcaa cccgataaag gcgcataagt
    10981 aaattctccg gtcggaccac caacatgaac atataatgga aacactgtat ccacaacaca
    11041 tccgaagtta ttagttacag aaagactgat gtcataatca cccggatcat aataaacatg
    11101 ctgcggattg gccgttgtac tggtatttga agttccgctt gcagaatcac caaaattcca
    11161 cagatattga ataacatcgg acgacgacgt attggaaaag tccaccacca gcggtgggca
    11221 gagtgtactc actactggcg ccgtaaaatc gggaaatggt atggatacgg tgatatccaa
    11281 tgaataagtg ctatcgcaac cattagcgtc tgtagcaacc agcgttactg taaaaaccgt
    11341 tgtcgtatca accaaataat gatgcactgg atttgttaga gaacttgccg gactgccatc
    11401 accaaaatcc cattcataag aaatggcggc agtactggtg ttgacaaatg taaccgtttc
    11461 ggaataacaa aacacattgt ttgcatgagt ggtatgaaag gagggatttg gttttgttgg
    11521 ctgaatataa ttatagaaaa ttcttgtact gtcgcatcct ttcgaatctg taaccgttag
    11581 cgacaaattg taattccctg ctgtgtagta ggtgtattgc acagtaggat gagctgttga
    11641 cgtagatcca tttccaaaat tccataacca atcggtcaaa acagccgggg cagcagcact
    11701 gctattgtcg gtaaattcaa cagtaagtgg agtacatcct gtcagtggtg tagttggtgt
    11761 aaaatttggc gaaggaagct gattgacaat aataacactt gttattgtgg tatcatcagt
    11821 acagccccaa ctgtcagttg caagtaatgt tacgtcataa acacctgctg tattgtaact
    11881 atgactaacc gacaccggag ctccggaaga tgtggcgccg tcgccgaatg tccatgcacg
    11941 atttgtgatc gcaacatgcg ctgtagataa atcagaaaac accaccggat cgtacataca
    12001 gacttcagga atattggact ggaaattggc aaaaacctca tctacccgca cctgaacctc
    12061 aatcgagtcg cgacagccat ttgagtttgt taccagcaat tcaaccatat aagtccccgg
    12121 agcagcatat gcataatctg gattctgaac agtgtccgta ccaccgtcat cgaaggtcca
    12181 gagccatgtt tcggcaccga cagactgatc ggtaaaatgc actgtatctg gatacgaaca
    12241 cagcaaagat ggtgatggag aaaaagcagc aataggtggc cagataacaa tataatcttc
    12301 tttttgcaac gtgtcgcgac aaccattatg tgtagccaca agagaaactg tgaatgttcc
    12361 ggtatcacca tactgatgtg tcggatcagg gccactgcca aactgtccat cgccgaaatc
    12421 ccaattccaa ctttcaacaa atgcactgga gctgtcatga aatgccacca tctcactata
    12481 acaaacaatg gtgtcctcgg caaaaaaacc tacctgcgga tgtaatccgg ccatgatgta
    12541 attgctcatg gtgatagtat tggtgcatcc ttcggtattg atgatggaaa gagtaacatt
    12601 aaaaacgccg gaatcaccat aagtatgcaa aggattgact gcagtatcca atggcgagcc
    12661 atcgccataa tcccagatat atgtagtgcc tgcaccgccc gaagatgaag taaactgcac
    12721 gtcaagcggg acacaaccag aaagaatatc agccgaaaaa gttatcggag gcggagcaat
    12781 ctgcacatag tttggttttg taagcgttcc aacgcaacca ttaatatcag ttacagtaag
    12841 agtaacagaa taatttccaa aagcattgta tgtattcgaa gcattggccg aatcaggccc
    12901 tccgaaactc caaacataat ttacatagcc cggatcagcc ataaaatcaa cagtaaacgg
    12961 caccatgcaa cctaccggat cgggcgatga aaaatcaaaa accggtgtcg gaaaaaccac
    13021 aacagtatct gtaaattgag tatccatagg acatccatag gaatcaacgg cagaaaatga
    13081 tactgtataa atacctgaat tcgtataggt atgagacgga tttaaaaccg tggaagtgcc
    13141 cccatcacca aaatcccaca aagccgatgc aataccggta ttcaaacatt gaaagtttac
    13201 cggaatcggc tcgcaaccag aatcaggacc aaacgaaata tcaggaaccg gcacaacaat
    13261 attgacaaaa ttgttcatta ccaacgtgtc tgaacatcca tttgcattta tcgcaataag
    13321 cgttacagaa tactgccccg tgttatattg atgcgaagga ttctgatctg tgcttgtatt
    13381 accgtcgccg aaatcccaga aatatgataa tcccgaacct gttgaagtat tgttgaaatc
    13441 tacagatgcg ggagaactgc aggaaatgtt gtttgtgcta gtaaatcccg caaccggatt
    13501 gggataaaca gtaacataac tattcatcgt ctgattggaa gaacaaccat aaatatctgt
    13561 aaccagcaat atgatattgt atgtgcccgg attggtgtat gtatgtgaag gatttcccga
    13621 aacagagctg aatccatcac caaaattcca attccagctg gctacaggcc cgtcgcccaa
    13681 agcagtcata tcattaaact gcactgtcaa tggctggcat ccgctaacag gaaatgcaga
    13741 aagatcggcc accatttttt tatgaacaac aatataattg gtacgggtaa aagtatttga
    13801 accattggca ttggtcacct taagtttaac agtataagta ccattggaag aataaacatg
    13861 cgtaggattt tgtaaagcag agtgtgtggt tccatcacca aaatcccaat cccagctggt
    13921 tggattgcca gtgctttgat cctgaaaaga cactgaaagg aagccacaac ccgatgtggg
    13981 cgtaatggca acaaaattgg caacaggtgt ttgcgcctgc aacagcaaaa cattgcaaaa
    14041 aacaataaca gtaaaaagaa atttcttcat aagcagcgtt tttcttggca gtatgaaaca
    14101 gaggcaaaac acaaccctgg aaaagcacat aacatttgat gacggtacaa cgacaatgtt
    14161 taatcatcaa attgcaaata ttcacatatc tgaatgcgtt ttatttgaaa gaagatgcag
    14221 ttagacctga caaaagaatt gatttgaata aaaaaaagac atctttgttt gaattcaata
    14281 atttaataaa aaaccgatat gcagaaatct cccaaacaca catttttaaa gctttttgaa
    14341 aattcttgaa caataactgc gtaataatca aaaatattta tacctttgca gcccaattta
    14401 ataaccaatc gaaaggagaa tcgatttatg atcattgtta cactgaagga aggtgacacc
    14461 attgacaaag cgttgaagaa actgaaacgt aaatttgaaa agacaggcgt tgcccgagaa
    14521 ctgcgcgacc gccagaaatt taccaaaccc tccgaacgca agagagaggt tcagtccaaa
    14581 gctttgtacg ttcagcgaat gatttcgaaa caaatgaacg ggtagcgctt gacttttgag
    14641 taataaatta ctatctttat cccattaatc accggttagt gggatttttt tatgatagag
    14701 cggttcatca catacctcac caaggaaaaa agatactctt tacacactgc catttcttac
    14761 cgcgccgacc tgaattcatt caggcaatat ctggataaga attatgacgg cgttgcattt
    14821 gaaaaatgca ctccggttat cattcgttcg tggcttgcag aaatggcctc aatgaattac
    14881 aatccgcgca gtctgcgcag aaaaaggacc gcgctgtctt cgttttatcg ttaccttata
    14941 tatataggac ttacagagac aaatccgaca cgcgggattc cgctccccaa gctccctgta
    15001 cggcttccgg tctttgctga tgaaaaatcg gtgcagacta ttcttgcatc aggcgaaaag
    15061 aacaatgatt atccaaccgt gcgcaaccag ctgatattcg agatctttta cgccaccggc
    15121 atgcgactca gtgaactcat cggcataaaa aacacagaca ttgattatgg taagtgcacc
    15181 ataaagatat tagggaaaag aaataaagaa cgaattgttc cgttcagtaa gcaattgctg
    15241 gagcatatga acgcatacga atcattgaaa aaagaatggt ttgatgatct ggaaacagat
    15301 tccaactact ttctaaccaa tacaggccgc aaactatatc caaaatatgt ttacagggcc
    15361 atccaccaat cgttggaaaa tgtaacatcg ctgagcaaga aaagccctca cgtgatgaga
    15421 cacacctttg cgacacacat gctacagaac ggagcagatc tgaattcgat caaggaaatg
    15481 cttgggcatg ccaaccttgc agccacacag atatacacac acacgaacat caagcaattg
    15541 aaagaaatct accataagtc acaccctaaa tcctaaaaca aaggaggaaa aattatgaaa
    15601 atcagcatca gttcagtaaa attcaaagct gacaaaaagc ttgaagactt tatccaggac
    15661 aaaatcggga aactggctac aaattacgac aatttgataa gcggggaagt tacactacga
    15721 ctggataatt cggcaaatca ggaaaataaa atcgccgaaa tcaggctaaa tattccaggc
    15781 aacgatttat ttgcaaaaaa acagtgcaag acatttgagg aggcgacaga ttcagcgctt
    15841 gatgcattaa gaaagcaatt gtctaaacat aaggagaaaa tacacccata aaaaatattt
    15901 tttcagaatt atttggtgta ttaaaaaaag tttgtacttt tgcagtccct ttttgaggga
    15961 ctgctttttt taataagagt aaatcaataa gggatcgttg tttaacactg tgtatcaaca
    16021 ttttcaaatg ccgatgtaac tcagttggta gagtagcggt tttgtaaacc gcctgtcggg
    16081 ggttcgagtc ccttcatcgg ctcttttttg aaaatgaaaa aataatgggg agattccaga
    16141 gtggctaaat ggggcagact gtaaatctgt tgggcaacct tcggaggttc gaatccttct
    16201 ctccccacaa acaacaagcg gaagtagctc atttggtaga gcgaaagcct tccaagcttt
    16261 aggtggcggg ttcgagcccc gtcttccgct cgagaattgc ctttgtagct caggggtaga
    16321 gcacttcctt ggtaaggaag gggtcgggag ttcaactctc cccaaaggct ctttcagtat
    16381 ttaaaacaag cagtaacaat aaacagacaa acagcatggc aaaagaaaaa ttcgatcgca
    16441 gtaagccgca cattaacatc ggtaccatcg gccacgttga ccatggtaaa acgacgttaa
    16501 ccgccgctat cactcttaat cttgcagata agggattcgc agaattcagg tcattcgatt
    16561 caatcgacaa cgctcctgaa gaaaaagaac gtggtatcac catcaacact tcacacgttg
    16621 agtaccagac cgacaaccgt cactacgctc acgttgactg tccaggtcac gctgactacg
    16681 ttaagaacat ggttactggt gctgctcaga tggacggagc aatcctggtt gttgccgcaa
    16741 ccgacggtcc tatgccgcag acccgtgaac atatccttct ggcacgccag gtaggtgttc
    16801 ctaaaattgt ggttttcatg aataaggttg accttgttga tgacgaagag cttctcgacc
    16861 tcgttgaaat ggatattcgc gaccttctga gtttctatgg tttcgacggt gacaacaccc
    16921 cgattatccg cggatcagct cttggtggac tcaataaaga acctaaatgg gttgaaaaag
    16981 ttatggaact gatggaagct gttgacaact tcattccaat cccaacccgc gcagtagaaa
    17041 aagacttcct catgccagtt gaagacgtgt tctcaatcac cggtcgtggc actgttgcta
    17101 ctggtcgtat tgaaaccggt gttatcaact caggtgaagc agttgatatt ctcggactcg
    17161 gtgccgaaaa actgaaatca gttgtaaccg gtgttgaaat gttccgcaaa attttggatc
    17221 gtggtgaagc cggcgacaac gtaggtcttc tcctccgcgg tatcgacaaa aacgaaatcc
    17281 gtcgtggtat ggttatcgcg aaaccgggtt ccatcaaacc tcacaaacaa ttcaaagctg
    17341 aagttgtgat tctgaaaaaa gaagaaggtg gacgtcacac tccattccac aacaaatatc
    17401 gtcctcaatt ctatttccgc accactgatg ttaccggcga agttgttctt ccggacggtg
    17461 ttgagatggt tatgcctggc gacaacctct ccatcactgt taatctcatc accgagattg
    17521 caatgaacca gaacctccgt ttcgctattc gcgaaggtgg ccgtacggtt ggtgcaggtc
    17581 aggtaactga aattctggac taataaaaca acctttggta aagagtattc gcacacaaac
    17641 aggtgcggat actttttaca cacgggtgta gctcaattgg tagagtggcg gtctccaaaa
    17701 ccgtaggctg tgggttcgat ccctaccacc cgtgcaaaat aaaggaagaa aaaatcaaaa
    17761 gatgcgaaag ataataaata gtctcagaga agttcgcgat gaaatgctga acaaagtcag
    17821 ctggcccaca tggctagaac ttacagaaag cgctatcgtg gtttcgattg cttctctcat
    17881 cattgcaggt gtagtacttg taatggacat ttcttttgag aatattctaa aactggttta
    17941 cggactcttt tagtacaaag cacccatgag cagcgaagaa tcaaagaaat ggtatgtggt
    18001 ccgtgcagta agcggaagcg aaaagaaagt caagcaatac attgataatg agattgcacg
    18061 gcttggactt caggatgctg tagcacaggt tttaatacct atggaaaagg tgttccagat
    18121 tcgcaaaggc aaaaaagtga ccaaagaaag aaatttcttt ccgggttaca tacttgttga
    18181 agctgctctt gacagtgaaa tagaacacat tataaagaat gtccctggag tacttggctt
    18241 tctcggaagt aaaggagaag ccatcccgat gagacagaat gaagtaaatc gcatattggg
    18301 aaaagttgat gaactggccg ataaaggaga agaaatgtat gagacattcc tggtcggaga
    18361 atcggtacgt gttatcgacg gtccattcaa cagtttttca ggtgttattg aagaagtgaa
    18421 cgaagagcgt aaaaagctca aagtcatggt aaaaatattc ggcagaaaaa ctcctcttga
    18481 gctgagtttt atgcaggttg aaaaagaata ataaacgagt aaaaagaaaa atctcatggc
    18541 aaaagaagtt gctggattaa ttaagctgca aatcaaggga ggggccgcca atccttcacc
    18601 acccgtagga cctgcattgg gtgccaaagg tgtgaacatc atggaattct gcaagcaatt
    18661 taacgcacgt acgcaggatc aggccggcaa gctgctcccc gttattatta cggtatataa
    18721 agataagtca tttgacttta tcgttaaaac gcctcctgta gcagtacagt tgctggaaaa
    18781 agcaaaaatc cagaaaggat caggcgaacc aaaccgcgac aaagtggcct cgattacctg
    18841 ggaacaggta caggcaatag cagaagccaa aatggttgac ctcaactgtt tcactgttga
    18901 atcagccatg cgcatggtag ctggaacagc ccgcagcatg ggcataaaag ttaccggcac
    18961 tgcccctttc caaactaatg cataataaaa aaaagaactg acatggcgag aatttccaag
    19021 aaaagaaaag aagccttaag caaattcgac aaaactgctc aatatacgtt gaccgacgca
    19081 ttgaagcttg tgaaggagct tacctacaca aaattcgact catcggttga cctcgatgtc
    19141 cgtctgggcg ttgatccccg caaagcgaat caaatggttc gcggcactgt gacgcttcct
    19201 catggtacgg gaaaaactgt ccgggttttg gttctctgcg gtcctgaaaa ggaagaagaa
    19261 gccaaagcag ccggcgctga ccacgtaggt cttgatgatt atatcgccaa gatcaaaggt
    19321 ggttggaccg acgtggatgt tgttattacc actcccaatg taatgcccaa agttggggcg
    19381 ctgggtaaaa ttctgggacc acgtggtctc atgcccaacc caaaaacagg cacggtaacc
    19441 atggaaatcg gcaaagctgt tgccgatgtg aaagccggta aaattgattt taaagttgac
    19501 aaatatggta tcatccattc ttctgttggc aaatcttctt tcacagttga aaaactggaa
    19561 gacaatgccc gcgaattatt gtcaaccatc atcaaactga aaccgactac caccaaggga
    19621 acctatgtga agagtgtgtt tttatcgagc acaatgagtc cggggataca tattgatgtt
    19681 aaaagtgtct ccaactaacc cctaacagaa agttcaactt atgaaaacaa aggaagacaa
    19741 aaaccaaatt gtagctgagc tgactgaaac actgaagcag tacgatgttg tgtatgtagc
    19801 cgacatttta ggtctgaatg cagaacaaac ttcgtcattg cgcagaatgt gtttcaaacg
    19861 cgacattaag ctgcaaatgg tcaaaaacac cttactgaaa agagctttgg aaaattccgg
    19921 cagggatttc tcagaactta ttccggtatt gaaaggctct tccgctgtga tgttgtcaga
    19981 tgtgccaaaa gcacctgcag cgctgattca ggaattccgc aagaaaacca aattaccgtt
    20041 gctcaaggga gcttatgtga tggaaatgac cgttattggt gacaatcagc tcggattcct
    20101 tgccggaatc aaaacaaaga acgagcttgt tgccgatgtg gttcttgccc tcaaatcttc
    20161 agccatcaaa gtggctggcg ctctgaaaac cattgtggac aaagacgccg cgtaatttaa
    20221 atgtttaacc gtaaaaaaag gaaataaaaa tggcagattt aaaagctttt gctgaacagt
    20281 tggtaaacct gactgtgaaa gaagtaacag aacttctcca gatactgaaa gacgattacg
    20341 gaattgaacc agctgctgca acagtcgttg ctgctgccgg tggttccgct gacgcagccg
    20401 ctgaagaaaa gagcgaattt gatgtaattc tcaagagcgc aggacaggca aaactcgcag
    20461 ttgtaaaact cgtgaaagaa cttaccagtc ttggtctcaa agaagccaag gaacttgttg
    20521 acgctgctcc aaaggccatc aaggaaaaag tcggcaaaga tgaagccgat gctctgaaaa
    20581 aagcactcga agaagccgga gcagaagttg aagtcaaata acttctgacc tgctttgcct
    20641 ggttaagacc tctttcagtt gtcagaggtc ttaaccattt aatataattc attgtcgatc
    20701 aacataaaac cgtactgcat tgacaacaaa ccccaaaaac cagagagtca ctttcgcgtc
    20761 aacctctcag cccgattatc cagattttct tgaaatccag gtcaagtcct ttcaggactt
    20821 tttccaattg gaaactactc ctgacaacag aaaaaatgaa gggctataca aggttttcgc
    20881 cgaaaacttt cccatttctg ataccagaaa caattttgtt ctggaatttc ttgattatta
    20941 tatcgacccg cctcgctatg ccatcgaaga atgcattgag cgcggtctga cgtacagcgt
    21001 acctctgaaa gcaaagctga agttgtactg tacagatcct gagcatgagg actttgaaac
    21061 catcactcag gacgtttatc ttggcatgat tccgtacatg accgataaag gcacttttgt
    21121 tatcaatggt gccgaacgcg ttgtcgtttc tcaacttcac cgcagccccg gtgttttctt
    21181 cggatcaagc cgccacccta acggatcgat tctttactct gcccgtatca tccccttcaa
    21241 gggctcgtgg atggagtttg ctacagacat tcataatgtc atgtatgctt acatcgaccg
    21301 taaaaagaaa ctgcctgtta ccactctgct acgtgccatt ggctgggaaa gcgacaaaca
    21361 aattctcgaa ctctttgatc tcgccgaaga aattaaggtt tctaaagctg ccatcaaaaa
    21421 agaagtcggc cgcagactcg ctgccagggt tgttaaggcc tgggttgagg atttcgttga
    21481 tgaagatacc ggtgaggttg tttctatgga acgtacagaa gtaattctgg aacgcgaaac
    21541 cattattgaa gaaaagcaca ttgatatcat tgccaacagc ggcgtgaaaa ccgttttact
    21601 tcataaggaa gatgcggcaa atgccgacta cagcgtgatt ttcaatactc tgcagaagga
    21661 tacagccaat aatgccaaag aagctgtcga atatatttat cgacagttgc gtaacgcaga
    21721 accacccgat gaagaaaccg cccgaggtat catcgacaag cttttcttct ccgacaaacg
    21781 ttacgatctg ggtgaagttg gccgatacag aatcaaccgc aaacttggtc gtcagaaaag
    21841 cgaagaccgc attctgacca aagaagatat cattgatatt ataaaatatc tggttgaact
    21901 catcaacagc aaagctgagg ttgatgatat cgaccacctc tcaaaccgcc gtgtaagaac
    21961 ggttggagaa cagttgtacg ctcagtttgg agtgggtctt gcacgtatgg cacgtaccat
    22021 ccgcgaacga atgaatgttc gtgacaatga agtgttcaaa ccgattgacc tgattaatgc
    22081 caaaacgctt tcttcggtca tcaattcttt ctttggcacc aaccagctgt cacagttcat
    22141 ggatcagacc aatcctctgt cggagctgac aaacaaacga cgtatttcgg ctctcggacc
    22201 tggcggtctt tcacgcgaaa gagccggctt cgaagtccgt gacgttcact acacccacta
    22261 cggccgtctg tgtaccatcg aaacaccgga aggtccgaac atcggtctta tttcttcgct
    22321 ctgcgtttat gccaaaataa accagcttgg attcattgaa accccatatc gcaaggttac
    22381 caagggtatt gttgatctca aagctcttcc ggtttatctt tcagctgaag acgaagaaga
    22441 gaaaattatt gcccaggcaa gtactccaat cgatgaaaaa ggacatttca tagaagatcg
    22501 gattaaatca cgtcacttgg ctgattatcc aattgttgaa aaaaccgatc tcgatatgat
    22561 ggatgtggca cccaatcaaa ttgcatcgat tgcggcctca ctcatcccat tcctcgaaca
    22621 tgacgatgcc aaccgtgctc tcatgggctc gaacatgatg cgccaggccg ttccgctcct
    22681 ctgccccgaa gcacccattg ttgctaccgg actggaagga aaagtagctc aggacagccg
    22741 tgttctcatc aatgccgaag gcgatggagt ggttgaaaaa gttgatgccc gccaaattac
    22801 catccgttat catcgcaacg acacagacaa gctggtttca ttcgaagacg atatcaagag
    22861 ttatcatctg accaagtttg ccaaaaccaa ccaaagcaca accatcaatc tgaaacccat
    22921 tgtgaaaaaa ggacagaaag ttcacaaagg acaggtgctt tgcgaaggat atgctaccga
    22981 caacggcgaa ctggcgcttg gacgaaatct actggtagct ttcatgccgt ggaaaggtta
    23041 taacttcgag gatgctattg tgatttcgga acgtgtagta cgtgaagatg tcttcacttc
    23101 tatccatatt gaagaattca tcatggaagt ccgcgatacc aaacgaggta ttgaggaact
    23161 caccagtgac attcccaacg ttagtctcga agccattaaa aatctcgatg ccaacggatt
    23221 aatccgggtt ggtgccgaag ttaaagaagg cgacattctg attgggaaaa tcaccccgaa
    23281 aggtgaaacc gacccaactc ccgaagagaa attgctccga gctattttcg gcgacaaagc
    23341 cggagatgtg aaagacgctt ctctcaaagc gcctcccgga gtaagtggcg ttgtgattga
    23401 caaaaaactg tttagtcgtt ccatcaagga taaaaaagca aaaaccaaag aaaaatcaac
    23461 ccttgatacg cttgaaaacg aattcgaaca tcaggtttcg gagattcgta aacgtttgat
    23521 tgaaaaaatc cttgtaatta ccgaaggaca ggtttgtcag ggtgtttcga acaatctgag
    23581 acaggaagtg atttcgaaag gaaccaaatt cactgccaaa gcgctaaaca acattgattt
    23641 catcaatatc attccaacca actggaccaa agatgcgaag gccaatgaac tgattcaacg
    23701 cctgcttcat aactttgaaa tcaagtacaa ggaagttctt ggcgtttatc agcgcaaaaa
    23761 atttgttacc caggttggag atgaactacc gacaggcatt gtacagctgg ccaaggttta
    23821 cattgcaaaa aaacgtaaac tacgtgttgg agataaaatg gccggacgtc acgggaacaa
    23881 aggtattgta gcccgtattg tccgtgatga agacatgcca tatctcgaag acggaagccc
    23941 ggtagatatc gtgctgaacc cacttggtgt acccagccgt atgaacctcg gacagattta
    24001 cgagaccatt cttgcatggg ccggtcagaa actggggacc aaatttgcca cacccatttt
    24061 cgatggagca tctgtggatc agatcaacgg ttaccttgaa aaagcaggac ttccgaaaaa
    24121 cggcaaaacc tatctgcgcg atggtggcac cggcgttcca tttgatcagc cggccactgt
    24181 tggtgtaatc tatatgctta aactacatca ccttgttgat gacaaaatgc acgcacgttc
    24241 tattggccca tattcactga ttacccagca acctcttgga ggtaaagctc agtttggtgg
    24301 tcagcgtttt ggagaaatgg aagtctgggc tctcgaagct ttcggggctg cccatatcct
    24361 gcgtgaagtg ctcaccatca agtctgatga cgttatgggt cgtgccaagt cgtatgaatc
    24421 aatcgttaag ggcgagccca tgccaaaacc gggcatccct gaatcgttca acgtactcat
    24481 acacgaactc aagggtctag gactcaatgt tacatttgaa taaaacactc atcagaactt
    24541 tttaaaagtc gatatatggc atttagaaga gaaaacgcac caaaaagcaa cttttcgagc
    24601 atcattatca gtctcgcttc tccggaaaaa atcctggaga tgtcgtttgg cgaagtgacc
    24661 aagcccgaaa ccgtaaacta caggacatac aaacccgaac gcgatggttt attctgcgaa
    24721 cggattttcg gacctgtaaa agattacgaa tgccattgcg gaaaatacaa acgtatccgt
    24781 tataaaggta tcgtttgcga ccgctgcggt gttgaagtaa ccgagaaaaa agtcagacgc
    24841 gaacgcatgg ggcacattca attgactgtc ccggttgctc atatctggtt tttccgctca
    24901 ctcccgaaca aaataggttt gttactgggt ttaccttcaa aaaaactcga gcaaattatt
    24961 tattacgaaa agtacgtaat cgtcaatccc ggtatcaaag ctgcggatgg attcaaacaa
    25021 aacgattttt tgaccgaaga agaatatttc cagattatgg aaacgcttcc acgtgaaaat
    25081 cagatgcttg atgacagcga tccgaataaa ttcattgcca aaatgggtgg tgaagctttg
    25141 ttcgatttgc tttcaaaaat tgaccttgat gaactttccc cggctctgcg caacaaagcc
    25201 aacaatgaaa cctctcaaca acgtaaaacg gatattctca aacgtttgca ggtggttgag
    25261 tcttttcgag acggcaacaa acgcatcgaa aacagacccg aatggatgat tatcaaagtg
    25321 gttccggtca tcccacccga attgcgccca ttggttcccc tcgatggtgg ccgtttcgcc
    25381 tcttcggatc tcaatgacct gtatcgccgt gtgattatcc gcaataaccg tctgaagcga
    25441 ctgattgaaa tcaaagctcc ggatgttatt ctccgaaatg aaaaacgaat gctacaggaa
    25501 gcagtggatt ctcttttcga caacagccgc aaggtaaatg ccgttaaaac agaatcaaac
    25561 cgcgcactga aatcgctctc cgacagtctt aagggtaaac aggggcgttt ccgtcagaac
    25621 ctccttggta aacgtgttga ctattccggt cgttcagtta tcgttgtagg tccggaactc
    25681 aaactcagcg aatgtggtct tccgaaagaa atggcctctg aacttttcaa accgttcatc
    25741 attcgtaaaa tgttggaacg cggcattgta aaaaccgtaa aatcagccaa gaaaatcgtt
    25801 gaccgcaaag atcccgttgt ttgggacatt cttgaaaacg ttttaaaagg tcatccggtt
    25861 atgcttaacc gtgctcctac cctgcaccgc cttggtatcc aggcattcca acccaaactg
    25921 atcgaaggta aagctattca acttcaccca ctcgtttgta ctgcattcaa tgccgacttc
    25981 gacggtgacc agatggctgt tcacgtgcca ctgagcaacg aagctattct cgaagctcag
    26041 ctcctcatgc tgtctccgca caacattctc aatccggcca atggagctcc tatcgctgtt
    26101 ccttctcagg atatggtact tggtctttat tacatgacca aaggtcgcaa aagctcaaag
    26161 gaacatgtgg ttaaaggtga aggcatcaca ttctattcac ctgaagaagt cattattgcc
    26221 cacaacgagc aaaaagccga tcttcacgca atgatcaagg ttcgtctgac cgattctgaa
    26281 ggtaatgcga gcattatcga aaccacagta ggacgcgtat tgtttaaccg cgttgttcct
    26341 gaaaaaattg gtttcatcaa tactcttcta accaaaaaag cactccgcga tattatcggt
    26401 gaagttctga aaaacaccaa caatgtcgaa gctgccaaat tccttgatga tatcaaatct
    26461 ctcggattcc agatggcttt caaaggtggt ttgtcattca accttggtga tattattgtg
    26521 cctgcagaaa aggaaatttt catcaacgag gccaaaaatc aggtagatga agtgatgggc
    26581 aattacaata tgggattcat caccaataac gaacgataca accagattat cgacatctgg
    26641 acccacacca actccaaact caccaaggtt cttattgaca aattgtcaaa agaccgtgac
    26701 ggattcaatt ccgtatatat gatgctggat tctggagccc gtggttcaaa agagcagatc
    26761 cgtcagctct ccggaatgcg tggtttgatg gcaaaaccac aaaaagctgg tgccgccgga
    26821 ggtgaaatta tcgaaaaccc gattctttcg aacttcaaag aaggcctgtc ggttcttgag
    26881 tatttcattt ctactcacgg cgcccgtaaa ggtcttgccg ataccgccct caaaaccgct
    26941 gatgccggct accttacccg tcgtttggtc gatgttgctc aggatgtcat tatcaatgaa
    27001 cccgactgtg gtacattgcg cggactcact gtaacggcct tgaagaaaaa cgaagaaatc
    27061 gttgaaagtc ttttcgacag aatccttggc cgagtaacac ttcacgatgt tttcaacccg
    27121 attactggtg aactcatcat tgaagaaggt caggaaatta cagaagaata tgccaaagcc
    27181 atcgaagaag ccaacatcga agagatagaa atccgttcgg tactcacctg cgaatcaaaa
    27241 cgaggagttt gcgccaaatg ttatggtcgt aacctcgcta caggccgcat ggttcaaaaa
    27301 ggcgaagcag taggtgttat tgcagctcag tctatcggtg aaccgggaac tcagcttacc
    27361 ctgcgtacat tccacatcgg gggtgctgct tccaacgttt ccattgcctc gaagcttgaa
    27421 gcaaaatacg acggtcttct tgaaatcgat gagcttcgtt gcattgatac caaatctgaa
    27481 gatggtaaaa cagtgaaagt ggttatgggt cgttctgccg aaatgcgcat ccgtcatcca
    27541 aaaacacaag ctattctgca aagcgccaat atcccctacg gatcgcaatt gtttaccgaa
    27601 aacggacaac aaattacaaa aggcgatctc atctgtgaat gggatccgta caatgcagtc
    27661 attctttctg aagtttcagg aaaaatcatg ttcgaggatg ttcttgaaaa tattactttc
    27721 cgagaggaat ctgatgaaca aaccggtttc catgataagg ttattatcga aagccgtcag
    27781 aaagcaagaa caccagccat caaattggtt tcatcaacca aagaagaact caaacgctac
    27841 gatttacctg ttggcgctca tattaccatc gaagaaggca ccaaagtaaa tgccggcgat
    27901 gtgattgcca aaattccaag aacacttgga aaagcaggtg atatcactgg cggtctgccg
    27961 cgtgttacag aactcttcga ggcacgaaac ccgtcagatc cggctgtagt agcagaaatt
    28021 gatggtattg taagcttcgg aaccaagctc aaacgaggta ataaggaaat cattatcact
    28081 tcaaaaacgg gtgaagaaaa acattacctg atcaatacaa caaaacaaat tctggcacag
    28141 gaaaacgact ttatcaaagc cgggacacca ctgtcagacg gtgcaatatc tccgggtgac
    28201 attctcgcca tcaaaggacc aacaaaggtt caggaatatc tggtaaatga aatccaggaa
    28261 gtatatcgtc tgcagggtgt gaaaatcaac gataagcatt tcgagaccat cgttcgtcaa
    28321 atgatgcgta aagttgaaat tctcgacccg ggcgataccc gtttccttga aggcgaactc
    28381 gtgaacaaga tcgaattcca ggaagaaaat gatgccgttt tcaataaaaa atacattgaa
    28441 tcggctggtg acagcgaagt gctgaaagcc ggatccattg tttctgcccg caagctgcgc
    28501 gatgagaatt ccatgctgaa gcgcaaagat aaagttctcg ccgaagcccg cgatgctcaa
    28561 ccggcaacag ccaaccagat tctgcagggt atcacccgcg cagctctgca aacggaaagt
    28621 ctcctgtctg ctgcttcctt ccaggaaaca accaaggtgc ttaccgaggc tgccgtcaat
    28681 gccaaacgag atacactctt gggtctgaag gaaaatgtta tcgttggtca cctcatccct
    28741 gccggaaccg gtctgcgcga atacgatgac cttgttgtcg gatctatgga agaataccaa
    28801 tctctcctcg cctcaaaaga agaaacctta gaaaaagtct gatatggatg acaagaaaaa
    28861 acaggacaac tcattgaata tcgaactcac agccgatgtg gccgaaggca gctacgccaa
    28921 tctggccatc atttcgcact ccagcgctga gtttattctc gatttcgtga gtatcatgcc
    28981 ggggatgccc aaagcaaaag tgaaatcccg catcatcatg acacccaatc atgcaaaaag
    29041 acttctgaaa gcacttgctg aaaacgtttc gaaatacgaa aaaacttttg gcgagattac
    29101 agaacatgaa caggtgcctc cttatcagat gtttactcct gccggagagg cataaatcaa
    29161 atttatttca catgtacggg cgatgaggtt cgccccgaac cgccgaaagg ctgatgacct
    29221 ctgcagataa caatgcagag gtcatttttt taaattcatg aatatgctca attcaattgc
    29281 tgaaattatt attgcgggaa tgattgcatc gtggctctgc cggctcatcc gcattcccga
    29341 cttgattgga tttcttctga taggattgct gttcggaccc tatgtgctgc aaattcttga
    29401 tccttcattt accgcactat caggcgaatt acgcacagcc gcactcattg tcattttgct
    29461 tcgcgcagga tttgaactaa aaaaagacac cctgaaaaaa gtcggcagac ctgttttatt
    29521 gctggcattg ttcccacctt tatttgaaac gctggccgct gcaaccatat gcgtttatgc
    29581 cgccggactc tcgtggaatg aaggcctgat gctgggaatc attctcagcg ctgtttcacc
    29641 ggcagctgtc gttcctctca tgattcgctt ccgaaacgaa aaacgcggca tcaataagga
    29701 aatacccaca atggttctgg cagccagcag tctcaataat attcttgtac tcattgtttt
    29761 ttccattctc gttgtttttc ttgttcgcga tgaaatactc actgcatcag ccattacgag
    29821 tgtccctatt tccattgcaa ccggaattac catcggaatt gttagcgcgc tattgctgat
    29881 aaaaatattt gaccgcttca atccccgtgc tacccggcgt gtattgctta ttctggcttt
    29941 tgctgttgtg tttttacgac tcgaaaaaga attcgcccaa tgggtgccgt ttgcagctct
    30001 gctcgccgta atgacactcg gctacattct tctcaataaa cgtgagaaat atgcacacga
    30061 aatatcacaa aaactcgcta aaatatggat tttagcagaa atgatactgt ttacactggt
    30121 cggtgctgaa gttaatcctg aaatcgcaat aaaaataggg ttaaccggct tgctgattat
    30181 cggaggcggt cttctggtac gcagtttagt cacatggctc tgtctgaccg ggactaatct
    30241 gaacaagcgc gaaaaaggtt ttgtagtgat ttcctatatc cccaaagcca cggtgcaggc
    30301 cgccattggg gctgttccgc tggcactcac ccacacggcc ggcgccgaaa taattctggc
    30361 ggtggcggtg ctcagtattg ttgttacagc gccgctgggc gctattgcca ttgaaatttc
    30421 gggtaaaaga tggctggaga aagcgtaaaa agaaagagcc acaaggacac taaggcacaa
    30481 gaaatatttc gtgtctttgt gccttggtgg caaaattccc aaacgctcac cgaatcagtc
    30541 ttcgccgcag gcctgcctgt tgcagacagg tgaagaccaa ttctattcgc gtccaataag
    30601 acctgctgag ggatgcaaaa tttttcgtgt agattcgcgg aagaaaatag tggagaagca
    30661 gcgaagactt tagcagtgat tcttgcacca attgaatact gaatcatgaa cctgtctgac
    30721 tgcctgttgc agttaaactg gccaggcagg caaggaagta agaatttcga acggtactct
    30781 atataaattt tctccaaact tacttcttac ttccaacttc cgtgttcctt attcattatt
    30841 cattatttgt gtcttcctgc ctttgtggca aaattcgaaa gtccacaaaa aaacctccgt
    30901 acaaaagtac ggaggtctga tttcccccac attaagtatc ataacagaga cttaatgctg
    30961 aggtctcttc cggattcgaa ccggagtaaa cggttttgca gaccggcgcc tagccactcg
    31021 gccaagagac cttttttttc ggactgcaaa gatataaaat tatttgaatt ggcgcgataa
    31081 cacataaatg caattttctt ttctatattt gggcttatga ataagcaaat tcatctttca
    31141 gctggcaacc accggaatgt ggatgtggtt tttgtttcgt ttgaacgaga cgaaaagttg
    31201 attgccgctt tgaaaagtat gggtaatgtc cgatggagtg ccacgttgcg aaaatggtat
    31261 tttccgaaag cggcattcga tctttcaaaa ttgaaattgg ctatgaatgg gctggcaata
    31321 atcgatgata cattgcttaa agctgccact cataataagc caactgctca aagcgacgtc
    31381 attccctcag acattttaac aggattggct gaattcaaaa aatggatgct tcacaaacga
    31441 tacagcgatt caacgattaa aacatacgtt gaagctgcaa agagttttct tgtgttcata
    31501 cacccaaaac
//
LOCUS       DAOPKE010000029        30867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1566_length_30867_cov_14.696742, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000029 DAOPKE010000000
VERSION     DAOPKE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_30867_cov_14.696742"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1669
                     /locus_tag="PKY63_05535"
     CDS             146..1669
                     /locus_tag="PKY63_05535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPF00107.1"
                     /translation="MSDDLFKKIVSHAKEYGFIFPSSEIYDGLSAVYDYGQYGSELKN
                     NIRSYWWKSMVQMHENIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRADV
                     LIEDYAEKQFEKAEKEVEKAKKRFADNFDEALFRSTNPRVLEYRKKGEEAVTKMAQLL
                     EANDLEGVRQLIVDLEIVCPISGSRNWTDVRQFNLMFETRMGSVAEGAESIFLRPETA
                     QGIFVNFMNVYKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMELQFFVKPGT
                     EIQWYEQWREERMKWHLAMGIPASKYRWHKHEKLAHYANAAADIEFEFPFGFKELEGI
                     HSRTDFDLNAHQKFSGKKLQYFDPETNDSYVPYVVETSIGLDRTFLAVFSHAYEEQTL
                     EDGSVRTVLHIPHFLAPIKAAVLPLVKKDGMPEIAMKIVEDLRQDFMVQYDEKDAIGR
                     RYRRQDAIGTPFCITVDNETMENNTVTIRERDSMEQIRIPAEKLIYVLSEKLRPGKL"
     gene            1699..1878
                     /locus_tag="PKY63_05540"
     CDS             1699..1878
                     /locus_tag="PKY63_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPF00108.1"
                     /translation="MTTEEKILETMKKAGKAMSAGQIADATGIDRKEVDKVMKVLKTG
                     ARIESPKACYWQPKK"
     gene            1894..3471
                     /locus_tag="PKY63_05545"
     CDS             1894..3471
                     /locus_tag="PKY63_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPF00109.1"
                     /translation="MHKNIQPFIYALLLAAGLAGGYFLSSMSGRCDASLLKQGKIDEV
                     IQYAYDNYVDSIGYDALVDDAIAGMLEKLDPHSVYIPKLELDAVNDQLSGSFEGIGIQ
                     FRIESDTIIVIDPIANGPSARKGIMPGDRIVKVDGKNVAGIGIKNDDVLKLLKGPKGT
                     KVILGIFRKGVNGLRDYTIIRDVIPLNSLDAAFMVNQSTGYIKLNSFSATTYDEFVAA
                     MNELQPAGMKNLILDLRDNGGGYLDYAVDIADDFLPEGSTIVYTKGLHRKDKYYHSRN
                     GGRFEEGEVVILIDEFSASASEILAGAVQDNDRGLVIGRRSFGKGLVQEQHELRDGSA
                     FRLTVARYYTPSGRCIQRPYEEGYEEYYLDFYKSLLLNDSAAREYNLKKNDTLVYRTS
                     KGRPVYGGGGIIPDTLVLHDYSMSQSFQTGFFSSGKLFELVFQYVDGNRKILKAKYPD
                     IASFSSGFRVPEVVTNAVFAAGGINSATVAAHDKATGVAFIKANIARELFGIEAYYQI
                     IIHHDNMVKEALKYFGN"
     gene            complement(3468..3680)
                     /locus_tag="PKY63_05550"
     CDS             complement(3468..3680)
                     /locus_tag="PKY63_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00110.1"
                     /translation="MKYFFATTIIAALFLSSCGNSEKKEKEEMKAFEDSIKKDTSVNS
                     AVNAANDFLNDSAVSVDSASNTKSNR"
     gene            complement(3721..>3948)
                     /locus_tag="PKY63_05555"
     CDS             complement(3721..>3948)
                     /locus_tag="PKY63_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00111.1"
                     /translation="WDEYMEVYEKIFKTCSPEIPWTIVPADDKWYRNYLVAKHVVKAL
                     EALDMKYPKGYFDDENHRIMAEKALEDYKKQ"
     assembly_gap    3949..4048
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4049..4308)
                     /locus_tag="PKY63_05560"
     CDS             complement(<4049..4308)
                     /locus_tag="PKY63_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00112.1"
                     /translation="MLEKYSTTAPSTFDKTTIINETDRLVEKLRELQSVMYAENKNSM
                     LVILQGMDASGKDSTVKHVFGKINPMGVRVKPFKKPTPEEYA"
     gene            complement(4314..5273)
                     /locus_tag="PKY63_05565"
     CDS             complement(4314..5273)
                     /locus_tag="PKY63_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPF00113.1"
                     /translation="MTKGKSKKRIKLNELPVYNRMLDYSKGHKFPGSEGKSIYLVMSL
                     LLEGLKNSVLAMRASALAFKFMMAAIPALILINTALPLLLPEESKDILLSIFGTFLPR
                     QIFSSIAKPVSEAAGGFAGSVISITAITTLCFAVNGIRASIVIFNNSYHTPENRPLHK
                     QFGVALLLVFILLLLTIVAVPIFFLNQEVIEWLRFNNILGPFWSASLVFVVKYTFFFL
                     FITFILAAIYHLAPAKKKRLHLFSPGTIMAAFLTVMASIGFDAFISGFTRYEEVYGGI
                     SALFILMLFLFILSMIFLIGFEINAALYYGISIEKENPNSKQN"
     gene            complement(5266..6114)
                     /gene="nadC"
                     /locus_tag="PKY63_05570"
     CDS             complement(5266..6114)
                     /gene="nadC"
                     /locus_tag="PKY63_05570"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPF00114.1"
                     /translation="MHSSLTIEQIIDFALKEDIGDGDHTTLSTVPADKRGKMALFVKE
                     EGILAGVDLARQIFHHFNPDISMQIILRDGSHISKGDIAFLVDGPVQNLLTCERLVLN
                     FMQRLSGIATQTNKMVKILEGTQVKILDTRKTTPLLRELEKMAVRIGGGYNHRFGLFD
                     MILIKDNHVDFAGGITKALNQTKQYLTDKKLDLEIEIEVRNMEELREALEANIASRIL
                     IDNFKPHEMKEAVEFVNGRVQTEASGGITIDNLREYAETGVDFISSGALTHHIKSLDL
                     SLKALD"
     gene            complement(6117..6530)
                     /locus_tag="PKY63_05575"
     CDS             complement(6117..6530)
                     /locus_tag="PKY63_05575"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPF00115.1"
                     /translation="MKYFFILILSFFSLQGFSVNNDTLTIPDIYELNDKISDAIRSGD
                     ASKLASCFAPTIQLSVPGKKGDFSRTQAEIIMRDFFAQYPPSSFSVNSEGKTSGTNFY
                     TLGSYVSGSVRFKVYYVIQKADSEITLHILKFEIQ"
     gene            6607..7080
                     /locus_tag="PKY63_05580"
     CDS             6607..7080
                     /locus_tag="PKY63_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPF00116.1"
                     /translation="MKLSMLVVGKTRVGFVAEGLSFYGARIQKYFPWEMLETTDVKHI
                     PDVKAMVRTETGKTMKMIAPGDYVILLDEKGKQHTSLAFSLDLRKVIDSGSARRILFV
                     IAGAYGAGDELKQRANEIWALSKLTFPHQLVRLILAEQIYRAMTIMRNEPYHHEG"
     gene            complement(7122..8108)
                     /locus_tag="PKY63_05585"
     CDS             complement(7122..8108)
                     /locus_tag="PKY63_05585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPF00117.1"
                     /translation="MKILVYRISSIGDIILTTPVVRCLKKQYPHAEIHYITRKRYAET
                     ISSNPYISHLWLIEKSPLEILDQLKAEKFDYMVDLHRNWRSQRLMAHLNTKYNTFNKL
                     NIRKWIYTHFKINLMPDIHIVDRYFKAVKKLHVSNDKEGLDFFFPTDYNPEIKKNLPE
                     KYIAFAIGGTYATKRYPTPQIIKFVKLSPVPVVLLGGKTENNDAAEIESCIPDKVINL
                     CNKLSLTESAAVIDNSRLIISNDTGMMHIAAALQKPIISIWGNTVPAFGMYPYFPEGL
                     ENKSDIVENNNLRCRPCSKLGYKKCPKGHFRCMKDISPELIAEKARKLLGLQ"
     gene            complement(8518..9570)
                     /locus_tag="PKY63_05590"
     CDS             complement(8518..9570)
                     /locus_tag="PKY63_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF00118.1"
                     /translation="MKITPGNPRHLSIFIAVILALIVFAISLTVLELTTASKWIVALT
                     LAFTTFIVVFISLLYTLNRFIYNKIRLIYKSIRSTKLGKSDKKIQMEKGDVLENVSRD
                     VEQWTRDKANEIEVLKKNEEFRREFIGNVSHELKTPITTIQGYVLTLLDGGIHDDEIN
                     VKYLQRAAKNIDRLIAIVNDLDEIAKLESGTMKMNFSNFNFSALVKDVFEFMEIKAEK
                     RRIVLDYKNQSDSKTLVHADPDRIRQVLINLIDNSLKYGNRFGSTTISAFDMDEYFLI
                     EVADNGIGIDQADLPRIFERFYRTEKSRSRKLGGTGLGLAIVKHIIEAHQQTIHVRSK
                     IDVGTTFSFTLAKATK"
     gene            complement(9567..10265)
                     /locus_tag="PKY63_05595"
     CDS             complement(9567..10265)
                     /locus_tag="PKY63_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF00119.1"
                     /translation="MNSGENIKILLVDDEKDILEIMSYNLKKEGYDVYTARDGQSAIN
                     KALDIEPQLIVLDIMMPGMDGITACQEMRKHESLKQSVIIFLTARNEDFAQIAALESG
                     GDDFISKPITPKVFLTKVKTYLRRFSKDINPDTSRELEFEGLVINFERYIVIREGQEI
                     ILPRKEFELLALLASKPGKVFTREEIFSKVWGNDIVVGDRTIDVHIRKIREKIGLSLI
                     RTVKGVGYTFEIKK"
     gene            complement(10540..11856)
                     /locus_tag="PKY63_05600"
     CDS             complement(10540..11856)
                     /locus_tag="PKY63_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPF00120.1"
                     /translation="MKKLCFISLLVFAFLNTKGQTDTSHTAKMQWFTNAKLGIFIHWG
                     IYSTGRGSESWEFYNGHTSHKDYMAQINEFTAEKYNPAEWVTLIQKSGARYVVITSKH
                     HDGVALWDTKLNDLSTKKSSPAKRDVLTPFVNEIRKTDLKLGLYYSLIDWTNDHYTGF
                     LRDSSRYEINDEPKRWEKFMKFNNGQLSEILKLYNPDLIWFDGDWEHSAKEWNAAGIR
                     EMVLKNNPKTIINGRLQGYGDYATPEQHFPIIRPEKENWELCMTMNGSWGYYITDTAY
                     KTPYEIISIFADCISFGGNLLLDIGPKADGSIPEEQTFILEELGQWNAKHHEAVFESM
                     AGLPLGHFYGPTTIAQDSTAIYLFLQGKQSGNVILKGLNNEIQNIEVIGSNTPVPFNV
                     VGKISWSKVPGIVYINVPEGVQDKYMTVLKINLNGKLSLYRGEGGL"
     gene            complement(11966..12772)
                     /locus_tag="PKY63_05605"
     CDS             complement(11966..12772)
                     /locus_tag="PKY63_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00121.1"
                     /translation="MYLEPDKYYHIYNHANGFENVFRCNENYLFFIQKYRFHLLPFID
                     TYAYCLMPNHFHLLIRVKSREEMLESMEKILTPPTLHPGTYPETLPKFGTSAKLDKLD
                     KLEVKDLPDFFRLEKIAPSSRIKTYGKFLRKYYQLANIPPSDYATSDLFIGQLISRQL
                     GDLFSSYTQAFNKQFKRMGGIFIKNFQRKEVDSTNYLINLITYINNNPVYHGFANKTE
                     GWPYSSYNDVLNNRSDIVNCTTVIEWFKKLQNFINCHKTTPKIDFGYSFE"
     gene            complement(12796..14286)
                     /locus_tag="PKY63_05610"
     CDS             complement(12796..14286)
                     /locus_tag="PKY63_05610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPF00122.1"
                     /translation="MNFSFTDFLYSLQYLPGQPLIFQSVTFFILFAFFYLLYALSFNN
                     RSLRNAVLLIFSLYFYYKISGFFVVVMILMATIDFFIGKGIYKTKTTSGKKWLLVLSV
                     FMNVGALIYFKYTNFLIETWTGIAHPGTEPLILKIVQPLGISYFVFKSLTYIFDIHRE
                     VIEEPERNWFRYVLYISFFPNILAGPISKARDLLPQFISKPVIDKNILSKGLFLIMLG
                     LIKKIVFADYLAANLVDRVFESPQYFTGIDALMAAYGGMLQLYFDFAGYTDMMVGFGC
                     LMGFSIEHNFNKPFLAGNITEFWRRWHITLYNWLNEYLYQPLAFAWRRGGKTMILLAV
                     FITFFVSGLWHGAALTFVAWGLLHGAAIAWDVISQSTRNKIQQWMPKSIYSFISIFIT
                     FHFLVFTAVLLKVPNLVKAGEFYNFMLTTDFSMFPQWLSIYTTPFLLMLGGLILQYLP
                     VKLYAGMLGGFTKVHWSLKAATLAVLIILLYQLYSLDAMPFIYIQF"
     gene            complement(14288..16282)
                     /locus_tag="PKY63_05615"
     CDS             complement(14288..16282)
                     /locus_tag="PKY63_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPF00123.1"
                     /translation="MIKNALKIFVLLTGLLLFGLESHAQKNPADTNYLKQAVKYSEFY
                     DFLQYDKNMFEWYQIDAIAPFFEKLKKANKEKVVILHIGDSHIQSDIGTGITRAMLQQ
                     IFGFGGRGIVFPYQAAGTHSAFDYFAQAHGTWSASKNVELKPKMNIGVSGITVFTTDS
                     TAGFKLIFRKHYYSIQPDFKRIRIYCHKGKESFDAKIKAGGSNTWVDADCHSDTLPYV
                     EVILPKASDTLTLVVNKTSPEQKYFECYGIEIQSVANTGIQYISVGINGAGYHSFFNQ
                     NLTAAQLKAIKPDLVIFDLGINDFFRGTFNYQYIMSSINKAIALFRQSAPNAVFMLPN
                     AQDIYYRGRNITNCKDYSTLTRLLSKEQNVVLYDYFNISGGPRSMLNWAKNGLSQRDR
                     CHLSYKGYKVKGELYFNALLNSYIAYLSRKPDSLLVFNNAIDTTNFGKWVINKSTYYN
                     RQEIATTDKINNYKNQTGGTKYSNTGTGQYYIVRSGDNLSSIAKRYGVTVADLQRWNN
                     LSTTRINAGQKLLVSKSAASNTTQKTINQTSSQQTTIGSKKIVYTIRKGDNLSSIALK
                     YKVSVDEIKKWNGLNSNNIVAGKTLVIYASGTVPQTQTTTQKQNTTTQTTKTGTLHIV
                     KSGESLWSIAKKYNTTVEKIKKDNKLTSDKLNVGQKLSIK"
     gene            complement(16279..17745)
                     /locus_tag="PKY63_05620"
     CDS             complement(16279..17745)
                     /locus_tag="PKY63_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPF00124.1"
                     /translation="MKRYQVFSIIVLTGVLLLVASCFWPKDNIVLGEKVITFIPKEEI
                     LNFKVKDNTQNVDSVIAMLESRAYGLNEDAIKRMSILDESVFSSSDFFINPAGSNGST
                     ALENFFKYLQKSDSALIRIGHYGDSQIEGDRITSLLRIMFQDKFGGSGVGYVPIDDVT
                     SPVSYTRSTSPNWVRYTVFHNRSAKGLYCPGGTVFRYRFSTPESTRDSIGENGDTIKI
                     KVPACSYANATVFLKLFRGYSIARLWYGKATEPAELKVYNSKNNELLAEVVLDNPDPF
                     NVVKLPLKSNPVSLKFVFTGPSPEIYGLSFDPMSGVQFDNYGLRGHSGDGLMNIQNDF
                     LKAQFIKLNNKLVILQFGGNVTPYVKNEASLNTIKNSYDRLYAHFRKAMPDASVLVIG
                     VNDVARSVGGTYASYPMVSRIRDVQKEVALKNGCAFFDLYQLMGGENSVLAWNKKGWA
                     SRDGHYSDKGREIVVNELFLAIMGEYNKFLIHGTTAGK"
     gene            complement(17747..17992)
                     /locus_tag="PKY63_05625"
     CDS             complement(17747..17992)
                     /locus_tag="PKY63_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPF00125.1"
                     /translation="MNNEEINTKVQEIFRDIFQAPALIINPDMTANDVDKWDSLTHLT
                     MIAKVEETFGFRFKLKEMVKMKNVGDMLTIINEKLNV"
     gene            complement(17999..18682)
                     /locus_tag="PKY63_05630"
     CDS             complement(17999..18682)
                     /locus_tag="PKY63_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF00126.1"
                     /translation="MTELNYIIRRAKESDYAFLAEAILKADIGTTGTNTSYAALFGLS
                     YEQARDAITAMMPEEVEGCEFSPFHFLVAEFNGEPVACVSAWIEGLDGVSSWMARSAL
                     MQHYYPKGALDFVQSKKHITDKMMVHRTEGTMQIESVFVSDKHRGKGLAAKLIKAHVA
                     KFLLQQYPVSQAELMTYIENKTAIHTYEKIGFNIKDRTQCDDPDVLHYFPGTGMVLMQ
                     TDIMNLMKG"
     gene            complement(18675..20147)
                     /locus_tag="PKY63_05635"
     CDS             complement(18675..20147)
                     /locus_tag="PKY63_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF00127.1"
                     /translation="MQKTDKVIFPFLPESDDNLNAICIEDVFHSYTAFRSRIDAIAAE
                     IASRGYKKAAIGVVASDHIDTYAAAMAIWATGNTYVPLNPYHPAERIASIIEQAEIQL
                     ILHSAEKLEASATFVCTCDLKESTFNAPAVPANNDVAYILFTSGSTGQPKGVPVSYGN
                     VRSFLRNLEAMNYHLGPGSRFLQMFDLSFDLSIVSILSPCIFRGTLFSVPNNNIKYPT
                     IFRLLEEYEIEFAILVPSVVAYLRPYFDEIMLDKMKYMCLSGEAVPHGLTLEWKNCCP
                     NAEFQNLYGPTEATIYSVWYRMPENDIPSRNGIVSIGWPTLEIEAVIVDDENKILSDG
                     EKGELLLSGGQVIAGYLKNEEKNREAFVEVNGKKFYRTGDICIRENGLYFYLGRKDYQ
                     VKIRGFRVELSEIEHHIASFYENRRVVAMAAHDTTGNDVIVAAIEGSEDAVHELGEYL
                     KSKMPFYMIPSEFRFISAFPLNNNGKINRREINNLILGHD"
     gene            20214..20780
                     /locus_tag="PKY63_05640"
     CDS             20214..20780
                     /locus_tag="PKY63_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPF00128.1"
                     /translation="MRTLALLFIIFIVGPMLQAQTDTSLRAMNEREKELYKLLMDYRS
                     KKWLKSIPISPSLNKVAQCHVRDLNTYPRAKKCNMHSWSKNGPWKPVCYTDNHKGADL
                     AHIKPSELSSYTGEGFEIAYWNSSSDFTAKNALDGWKSSYGHNSMIINLGMWKKSKWR
                     AIGIAVEGNYAVVWFGKETDPAGEIIIK"
     gene            complement(20786..22204)
                     /locus_tag="PKY63_05645"
     CDS             complement(20786..22204)
                     /locus_tag="PKY63_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPF00129.1"
                     /translation="MFGLDLGMTLLLFGCILAAMAFEFINGFHDTANAVATVIYTRSL
                     KPRVAVIWSGIFNFLGVYFGGIAVAIGIINLLPLEILLDQNISHNIAMVASLILTAII
                     WNLGTWYFGIPCSSSHTLIGSIFGVGLAYLFISDSSGAALNWTKVIEALSALLISPVI
                     GFGLTILLMYIFRRTIRKKKFFKAADPEKKPPFWVRLLLIGTCTTVSYSHGSNDGQKG
                     VGLIMIILISLLPAHFALDNTKNPSNMIGNVNHIEYYIGKTDSTKILAADMPGYIALT
                     TSIADLKENLLIAQNPDEFDKLDRFNARKDILTIKKKTNEFLNSPSVVKNKALVLNHK
                     ESKMLKNDIKEMDSYTEYAPWWVILMVSLSLGLGTMIGWKRIVTTLGEKIGKSQMSYA
                     QGASAQIVATATIMSSSTIGLPVSTTHVLSSGIAGSMVYQGGWKNLQMKTIYNILLTW
                     VITIPVTIAMSGGLFLLIRSML"
     gene            complement(22672..24927)
                     /locus_tag="PKY63_05650"
     CDS             complement(22672..24927)
                     /locus_tag="PKY63_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPF00130.1"
                     /translation="MEYYFIGVLVILFLLSIMDLTVGVANDAVNFLNSGYGSRSLTFK
                     WLLVVASAGVFIGALFAGGMMDVARSGVFHPQYFMFEEVIMIYLAVMLNDVLLIDFFN
                     TLGLPTSTTVSLVAALLGASFSFAVAKLIENGQAFTDIDMYMNTEKAFAMIIAIFVSI
                     AVAFFFGLLIQKIARTVFSFNFSRYKIGGAIFGGLAIALISYFIFFKGMKSASFIDKP
                     MKEYVSANLLMILSFITAGTAALLLILQYLFRVNVFRVIVIYGTFAIAMAFAGNDLVN
                     FLGPSLAGLDAYNAYAATGDPSMTMESLSAPAKVPVGFLMGAGFIMLLTLWFNKKTRT
                     VIRTEVGLARQNEGVENFDTNPVARSLVKLALAIGNFFTLVRIPAVHRWIEKRFTAPP
                     MSNNPDAPAFDMVRAAVNLTVGSSLIAFGTSLKLPLSTTYVTFMVAMGTSLADRAWDR
                     DSAVYRVSGVTTVIGGWFITAFLAFLSAALLAYVLYLGSIVAVILLSALTVFVLIRSK
                     VIHRRREKEYQEKENMLRIDENTGEQEILDLCRNNVSFVIEKIPEVVRLIREGLEEEK
                     LKKLKEADEIVKDLNSRTELFSSTIGSSISALGDNYVLYGEYYLKNAEILREIAVSLG
                     YLTHPVYNHINNQHKNLGKNQMRDFESLSEDLQQLCDFSKQIILENSGRGLADNLHEK
                     ANVVCDKIKIVRRAQIERVKRKENASRSSLLFFNITTEIQNITRFVNELTELDIRTKS
                     QVAEADYRLID"
     gene            complement(25115..25939)
                     /locus_tag="PKY63_05655"
     CDS             complement(25115..25939)
                     /locus_tag="PKY63_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HPF00131.1"
                     /translation="MKNLFVIVLVGLLALSACQTEKIVEKTVVVHDTVYVNNFDYDYM
                     LMAVLWYQTSAEMRALCYQAFNTARISLDQTVASYKGTKKLAVVVDIDETMVDNSPYE
                     AQNVLGNFGYPERWGEWIEKASATAIPGAVEFMKYAVSKGVDVYYVSNRKIAEKDATV
                     KNMRALGFPMIDEDHLLLRDKTSSKNERRNKVSQTHEIILLIGDNMNDFSGIFENKSV
                     SDRFDVTDQNKSEFGKRFIVLPNPMYGDWEGAVLNYNFKQTNLQKDSVRKANLRGM"
     gene            complement(26001..29516)
                     /gene="nifJ"
                     /locus_tag="PKY63_05660"
     CDS             complement(26001..29516)
                     /gene="nifJ"
                     /locus_tag="PKY63_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPF00132.1"
                     /translation="MKNKKFITCDGNYAAAHVSYMFSEVSAIYPITPSSTMAEYVDEW
                     SANGRKNIFGDTVKVVEMQSEAGAAGAVHGSLQSGVLTSTYTASQGLLLMIPNMYKIS
                     GELLPGVFHVSARAIAAQALSIFGDHSDVMSTRMTGFAMLATGSVQEIMDIAPAAHLA
                     SIRSRIPFLHFFDGFRTSHEIQKVEYLDNDDVRSLVDMKALASFRQNALNPEHPVTRG
                     TAQNPDIYFQSREAANPFYDKVADIVEEYMVELNKITGREYHPFTYYGAPDAENIIIA
                     MGSVTETIRETIDYLNAKGEKVGLLTCHLYRPFSEKYFMKVFPKSVKRIAVLDRTKEP
                     GSEGEPLYLDIKNLFYNKANAPLVVGGRYGLSSKDTTPAQIISVYDNLKNTEPKNGFT
                     IGIVDDVTFKSLPQIAEINISDPSIFQAKFYGLGSDGTVGANKNSIKIIGETTNKYCQ
                     GYFAYDSKKSGGFTASHLRFGDVPIRSPYLVNTPDFVACHVPAYLGRYDMLRGLKKGG
                     VFLLNSIWDVEETKAHLPEDMKKYLAVNEIKFYTINATKIADEIGLGNRTNTIMQAAF
                     FKISGVIPYDQAVKEMKKFIDKSFGRKGDEVVKMNYAAVDRGGELTEVAIPAEWKNLE
                     GKTAFEDETHPDFIRNVADVINRQSGDDLKVSTFVGREDGTFPSGTTKFEKRGIAVTV
                     PEWQSENCIQCNQCAYVCPHAAIRPFLLTEAELAAAPAGTTTLKGLAATKEYNFRMQV
                     SVMDCTGCGNCVDVCPAKTKALVLKPLETQLAEKERWTYMDEKVGYKDTVLEKSKSVK
                     NSQFAQPLFEFSGACAGCGETPYIKAITQLYGERMIVANATGCSSIYGGSAPSTPYTI
                     NNKSGKGVAWGNSLFEDNAEYGLGIATGVKKMREFLTMKMKEAIAAGLGGDLKAACEE
                     WMAAIDNAEVSETASAKLLALAEKDNSELAKEIVDKKQYLIKKSVWVFGGDGWAYDIG
                     YGGLDHVLASGEDINVLVLDTEVYSNTGGQASKSSPTGAVAKFAASGKKTRKKDLGMM
                     AMSYGYVYVAQVSMGADQSQYLKAIREAEAYPGPSLIIAYAPCINHGLRSGMGKTQAQ
                     GKFAVESGYWHNYRFNPQLEAEGKNPFILDSKEPDWSKFQEFLKSEVRFTSLVQAFPE
                     EAKVLFAACEDNAKWRYNKYKKLAD"
     gene            29747..30628
                     /locus_tag="PKY63_05665"
     CDS             29747..30628
                     /locus_tag="PKY63_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00133.1"
                     /translation="MKNLIFSLVLLFPFFSLFGQTSDEKPAVRISDIMVQSGFQNFND
                     FSLGLNDFLKLAPGSPWLQNDFSDYSLSHASGNFTGLFSVSVGLKFAGKERKSYRANP
                     VLRLGIFDVRSSPDGVFYNKRESFPYDTLTSSATGNQYYVDSVSNMQYEMQYSTEHIG
                     LSASLQFGTNPQNRVSFYAGTSLVAALSVSNSTKLSKAEYVVFSSDDLYYSYPVYNNY
                     AYDYESVFIGNKSSMLLSMGIPMGIDFRLSRSHHFWSLIHLVYEFRPSVTMTSIPELK
                     NYVSTGMCNSMGFKVTW"
BASE COUNT         8456 a         6181 c         6842 g         9288 t
ORIGIN      
        1 taaacagcct ttatcaacag tgttgatgcg gtttttgagt ttttagagat gcccaaaaat
       61 agcaattttt attttgcagc aatgctttct gacaaaagca atacgatatt tattactttt
      121 gcatccactt taaacaatac atccaatgag cgacgacctt ttcaaaaaaa ttgtttccca
      181 tgccaaggaa tacggtttta tttttccttc ttccgaaatt tatgatggtt tgagtgccgt
      241 gtacgactat ggtcagtatg gttccgaatt gaaaaacaat atccgcagtt attggtggaa
      301 aagtatggtg caaatgcacg aaaatattgt gggaatcgat gctgccatat ttatgcatcc
      361 aaccacatgg aaggcctcgg gtcacgtgga cgcttttaac gatccgctca tcgacaacaa
      421 agacagtaaa aaacgctacc gcgccgatgt gctgattgaa gactacgccg aaaaacaatt
      481 cgaaaaagcg gagaaagaag ttgaaaaagc aaaaaaacgt tttgccgata atttcgacga
      541 agcgctcttc cgcagcacca atccacgcgt gctcgagtat cgcaaaaaag gcgaagaagc
      601 tgttacaaaa atggctcaat tgctcgaagc caatgacctc gaaggcgttc ggcaattaat
      661 tgtagatctc gaaatcgtat gccccatcag cgggtcacgc aactggaccg atgtgcggca
      721 gttcaacctg atgttcgaaa cccgcatggg atcggttgcc gagggtgccg aatcaatttt
      781 tttacgaccc gaaacggcgc aaggtatttt tgtcaatttc atgaatgtat ataaaaccgg
      841 ccgcatgaaa attccgtttg gtattgcgca aaccggcaaa gcgttccgca acgaaattgt
      901 ggcgcgtcag ttcattttcc ggatgcgtga atttgagcag atggagttgc agttttttgt
      961 gaaaccggga actgaaatac aatggtacga acagtggcgt gaagaacgaa tgaaatggca
     1021 cctggccatg ggtattcctg cctcgaaata ccgatggcat aaacacgaaa aactggcgca
     1081 ttatgccaat gctgcggccg atattgaatt tgaatttcct ttcggtttta aagaactcga
     1141 aggtattcac agtcgcaccg attttgacct gaatgcgcat cagaaattca gtggaaaaaa
     1201 acttcagtat tttgatcctg aaaccaacga tagttatgtt ccttatgtgg tcgaaacatc
     1261 aataggattg gatcgcacat tcctggctgt ttttagtcat gcttacgaag aacagactct
     1321 tgaagatggc tctgtgcgca ctgttttgca tattcctcat tttctggcac ctatcaaagc
     1381 tgctgtgctt ccgctggtga agaaagatgg tatgcccgaa attgccatga aaattgtgga
     1441 agacctccgt caggatttca tggttcaata cgatgaaaaa gatgccatag gtcggcgtta
     1501 ccgcaggcag gacgccattg gaacaccgtt ctgcatcact gttgataacg aaacgatgga
     1561 aaataatacc gttacaatcc gtgaacgtga cagcatggaa cagattcgta ttcctgccga
     1621 aaaactgata tatgtcttgt cggagaaact aagacctggg aaattgtaac tttgtgtaac
     1681 atcaaaaatc acactgttat gacaactgaa gaaaaaattc ttgaaacaat gaaaaaggca
     1741 ggcaaagcaa tgtctgcagg ccagattgcc gatgcaacag gtattgatcg caaagaagtt
     1801 gataaagtaa tgaaggtgct gaaaaccggc gccaggattg aatctccgaa ggcctgttat
     1861 tggcagccaa aaaaataatc ttatctccct gtgatgcaca aaaacattca accattcatc
     1921 tacgctttgt tgctggctgc cggactggcc ggtggatact tcctttcttc catgtcgggc
     1981 cgttgcgatg cgtctttgtt gaagcaaggg aaaatcgatg aagttattca atatgcttac
     2041 gacaattatg ttgacagcat aggctacgat gcgctggtgg atgatgccat cgcgggaatg
     2101 cttgaaaaat tggatccgca ctcggtttat attccgaaat tggagctcga tgctgtgaat
     2161 gatcagctca gcggctcgtt tgaaggtatt ggtattcagt ttcggattga aagcgacaca
     2221 atcattgtaa ttgatccgat tgccaacgga ccgagtgcac gtaaaggcat tatgccggga
     2281 gaccgtattg taaaagtgga tgggaaaaat gtggctggca ttggcattaa aaacgatgat
     2341 gtgctgaaat tgctcaaagg tcccaaagga accaaagtta ttcttggtat tttccgaaaa
     2401 ggcgttaatg gtttgcgcga ttataccatt attcgtgatg ttattccgct caatagtctt
     2461 gatgcggctt ttatggttaa tcaatcaacc ggatacatca aattgaattc gttttcggct
     2521 acgacatatg atgaatttgt tgcggctatg aatgagctcc agccggccgg tatgaaaaat
     2581 ctgattcttg atctgcgcga taatggtggc ggatatctcg attatgccgt tgatattgca
     2641 gatgattttt tgcctgaagg cagcaccatc gtttatacaa aaggattgca ccgaaaggac
     2701 aaatattatc attcccgcaa tgggggccgt ttcgaagagg gtgaggtggt gattcttatt
     2761 gatgagtttt ctgcctcagc cagtgaaatt ctggcaggcg ctgtgcagga taatgaccgt
     2821 ggtcttgtta ttggtcgtcg ctcttttgga aaaggactgg tgcaggaaca acatgaactc
     2881 agggatggat cagcgtttcg gttaactgtc gctcgttatt atactccaag tggcagatgc
     2941 attcagcgcc catatgaaga agggtatgag gagtattatc tcgatttcta taagtcgctt
     3001 ctgctcaatg attcagctgc tcgtgaatac aatctcaaga agaatgatac gctggtttac
     3061 cgcaccagca aaggccgtcc tgtgtatggc ggaggcggaa ttattccgga tactcttgtg
     3121 ttgcacgatt attccatgag tcagagcttt cagaccggtt ttttctcctc cggaaaattg
     3181 ttcgagctgg tgtttcagta tgttgacggc aatagaaaaa tccttaaagc aaaataccct
     3241 gatattgcat ctttcagtag cggatttcgg gttccggagg tggtgaccaa tgcggttttt
     3301 gctgccggtg gaattaattc agctaccgtg gcagcacatg ataaagctac aggtgtagct
     3361 tttataaaag ccaatattgc acgtgaatta tttggtattg aggcgtacta ccagataatc
     3421 attcaccacg ataacatggt gaaagaggca ttgaaatatt tcggaaatta gcgattactc
     3481 tttgtattcg aagcagagtc cacacttacg gctgagtcgt tcagaaaatc gttggcagca
     3541 tttacggctg agtttacact ggtgtctttt ttaatgctgt cttcaaatgc tttcatttct
     3601 tctttttcct ttttttcgga gtttccgcag gaacttagaa acaatgcggc aatgatggtg
     3661 gttgcaaaaa agtatttcat gtgttttttc tgtaaaatta aaaaatctgt tttgatctga
     3721 ttattgtttt ttataatctt cgagcgcttt ttctgccatt atgcggtggt tctcatcatc
     3781 aaaatatcct ttgggatatt tcatatcaag cgcctccagt gctttaacaa cgtgtttggc
     3841 caccagatag ttgcgatacc atttgtcgtc ggcaggaaca atagtccatg gaatttcggg
     3901 actgcatgtt ttgaagattt tttcatagac ctccatatat tcatcccann nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ggcatattct tcgggggttg gttttttgaa
     4081 tggttttaca cgcacaccca tggggtttat ttttccgaaa acatgcttaa cggtactgtc
     4141 ttttcccgat gcatccatac cctgtaaaat aaccagcatg ctgtttttgt tttcggcata
     4201 catcaccgat tgcagttcgc gcagtttttc aaccaaacgg tcagtttcgt taatgatagt
     4261 ggttttgtca aaagttgatg gtgcggtggt agaatatttt tccagcatgc agtttagttt
     4321 tgtttggagt tagggttttc tttttcaatg gaaataccgt aatacaaggc agcgttgatt
     4381 tcaaatccta tcagaaatat catcgataga atgaaaagaa aaagcatgag aataaataag
     4441 gcggaaatgc cgccatacac ttcttcgtat cttgtaaaac ccgaaataaa agcatcaaag
     4501 ccaatcgatg ccattactgt gagaaatgcc gccatgatgg tgccaggact gaacaggtga
     4561 agtcttttct ttttggctgg tgccaggtga taaatggctg caagtataaa tgtaatgaat
     4621 aaaaaaaaga aagtgtattt tacaacaaaa acaagactgg ccgaccagaa aggccccaga
     4681 atgttgttga aacgcagcca ctctatgact tcctgattga ggaaaaatat tggtacagct
     4741 acaatggtaa gcagtaacaa tatgaaaacc agcagcaggg ctacaccgaa ttgtttgtgt
     4801 aatgggcggt tttcgggcgt gtggtatgaa ttgttgaaaa tgacaatact ggccctgatt
     4861 ccattaacgg caaaacagag ggtggtgatg gcggttatcg aaatcacact gcctgcaaag
     4921 ccaccagctg cctcagaaac aggtttcgca atggatgaaa atatttgtct tggcaaaaat
     4981 gttccgaaaa tggaaagtag tatgtctttg ctctcttcgg gcagcaaaag tggtagcgcg
     5041 gtgttgatca gaataagcgc gggaatggct gccatcataa atttgaaagc aagtgctgat
     5101 gctctcatgg ctaaaaccga gtttttaagc ccttccaata acagcgacat gaccaaataa
     5161 atgcttttgc cttcgctgcc gggaaatttg tggcctttgg aataatcaag catcctgttg
     5221 taaacaggaa gttcgttcag tttaatccgt tttttgcttt tgcctttagt caagcgcttt
     5281 aagacttaaa tcaagacttt tgatatgatg tgtgagtgca ccggaagaaa taaaatcaac
     5341 accggtttca gcgtattccc gtaaattgtc aatggtaata ccgccactgg cctcggtttg
     5401 aacacgtccg ttgacaaact ctacggcttc cttcatttcg tgaggtttga agttgtcaat
     5461 cagaatgcgg cttgcaatat ttgcctcgag tgcttcacgg agttcttcca tattgcggac
     5521 ttcgatttca atttccagat ccagtttttt gtcggtcagg tattgctttg tctgattcag
     5581 cgctttggta attccaccgg caaaatcaac atgattatct ttgatgagta tcatgtcgaa
     5641 tagtccgaag cggtgattat aacccccacc tatacgaaca gccatttttt caagttcacg
     5701 cagcaatggt gttgttttgc gggtgtcaag tattttaacc tgagtgcctt caagaatctt
     5761 aaccatttta ttggtttgtg ttgcaatacc gctgagccgt tgcatgaaat tcagaacaag
     5821 ccgttcgcag gtcagcaggt tttgtacggg tccatcaacc agaaatgcga tatcaccttt
     5881 gctgatgtgg ctcccatcac gcaggatgat ctgcattgaa atgtcgggat tgaagtggtg
     5941 aaaaatttgt cgtgcaaggt caactccggc aagaatccct tcttctttga caaagagagc
     6001 cattttcccg cgtttatcgg caggaacagt gcttaatgtg gtgtggtcac catcaccaat
     6061 atcttctttt aatgcaaaat caatgatttg ttcaatcgtc agactgctgt gcatttttat
     6121 tgaatttcga atttgagtat atgaagcgtt atttctgaat cggctttttg aatgacataa
     6181 tacactttaa agcgtaccga tccggagaca tatgatccca gtgtgtaaaa atttgttcct
     6241 gatgtttttc cttcagaatt gacggaaaaa gatgaaggtg ggtattgtgc gaaaaaatcg
     6301 cgcatgatga tttctgcctg agtgcggctg aaatcacctt ttttgcccgg aacactcagt
     6361 tgaatggttg gtgcaaaaca ggaagccagt ttgctggcgt cgcccgatcg aatggcatcg
     6421 gatattttgt cattgagctc ataaatatca ggaatggtca gtgtgtcatt gttcactgaa
     6481 aatccctgca aagagaaaaa acttaatatc aaaatgaaaa aatatttcat tattttgtat
     6541 tttgccgttg caatctcaaa atttaagcca aagatacaat tttttgaaac taacccggat
     6601 caatatatga aattatccat gcttgttgtt ggtaaaacgc gtgtgggctt tgttgcggaa
     6661 ggattatcct tttatggagc cagaatacag aagtatttcc cctgggagat gcttgaaacg
     6721 actgatgtaa agcatattcc cgatgttaaa gcgatggtca gaaccgagac cggaaaaaca
     6781 atgaaaatga ttgctccggg cgattacgtt attctgctgg atgaaaaggg caagcagcac
     6841 accagtcttg cattttcgct cgatttgaga aaagtgattg attcgggaag tgcccgcagg
     6901 attttatttg ttattgccgg ggcttatggt gcaggtgatg agcttaaaca gcgagccaac
     6961 gaaatatggg ctttgtcaaa actaacgttt ccgcatcagc tggtgcgttt gattctggcc
     7021 gaacaaatct atcgtgcaat gaccattatg cgcaacgagc cctatcatca cgaggggtaa
     7081 agaatggata tactttgaag ggttctaaac gcttcgagag attattgcaa gccaagaagt
     7141 ttgcgggctt tttctgctat gagttccggt gatatatctt tcatgcatct gaaatgtcct
     7201 ttcgggcatt ttttataacc cagtttgctg catggccggc agcgcagatt attgttttca
     7261 acaatgtccg atttgttttc aagtccttca ggaaaatagg gatacatgcc gaatgcagga
     7321 actgtattgc cccatatgct tatgatgggt ttttgcaaag ctgcggctat atgcatcatg
     7381 ccggtgtcgt tcgaaattat aagccgcgag ttatcgatta ctgcagccga ttctgtcagc
     7441 gataacttgt tgcagagatt aatgactttg tcggggatgc atgattctat ttctgccgcg
     7501 tcgttatttt cagtttttcc gcccaacaga actaccggaa ccggactcaa ttttacgaat
     7561 ttgattattt gaggtgttgg gtatcgtttt gttgcgtatg ttccgccaat agcaaaggca
     7621 atgtattttt cgggcagatt ttttttgatt tcaggattgt aatctgttgg aaaaaagaag
     7681 tcgaggccct ctttatcatt gcttacatga agttttttta cagctttgaa ataacggtca
     7741 acaatatgaa tatcaggcat cagatttatt ttgaaatgtg tgtaaatcca tttgcggata
     7801 ttgagtttgt tgaatgtgtt gtattttgta ttcagatgag ccataagccg ctgactgcgc
     7861 caatttcggt gtaggtcaac catgtagtcg aatttctcgg cttttaattg atcgagaatt
     7921 tcaagaggcg atttttcgat aagccagaga tgcgaaatat atggattcga tgatatggtt
     7981 tcggcatatc tttttcgggt gatgtagtga atttcagcat ggggatattg ttttttcaaa
     8041 caacgcacaa ctggtgttgt cagaattata tctccgatgg agcttatgcg gtaaaccagt
     8101 attttcattc tttctggcat gaaaaatgag atttgaagaa tattatggat gttttcgcgt
     8161 taatttttta tcggaaatcg tccattccct acaaaattaa ttaaatttgc ataagggatc
     8221 attgagatga aaagaaaatc gtttatatac tttttgattg ttcttctggt ttgtgcagtt
     8281 gctgtttctt catgcaagac cgagaaaaag tataatccca aaaaatgtaa aacctgtccg
     8341 catttttgat tggcgcagcc cgcgtttaag tttgtaaagt ttaaaagtga aaagtttata
     8401 aaggtgcttt gtctataagc gtttcgtgtt acgattcagt tacctttcta ctttacaagc
     8461 tttatgaact ttcaacttct tatgccttgt gctttgagcg ttgagcaatg agttttatta
     8521 tttagtcgct ttagcaagag tgaacgaaaa tgtggtgccc acatcgattt ttgatcgcac
     8581 atgaatggtt tgctgatgtg cttcgataat atgttttaca atggcaagac ccaatcctgt
     8641 tccacctagt ttgcggctgc ggctcttttc ggttcgataa aaacgctcga agattcttgg
     8701 caggtcggcc tggtcaatac caattccatt gtcggccact tcaatcaaaa aatattcatc
     8761 catatcgaaa gcgctgatgg ttgtcgatcc aaagcggttt ccgtatttga gagagttgtc
     8821 aatcagattg atcagtacct gcctgatgcg gtctggatcg gcatgaacaa gggtttttga
     8881 atctgattga tttttgtaat caagaacgat gcggcgtttt tctgctttga tttccatgaa
     8941 ttcgaaaaca tcttttacca gtgccgaaaa gttgaaattg ctgaaattca ttttcatggt
     9001 gccgctttcg agttttgcaa tttcatcaag gtcgtttaca atggcaatca gccgatcgat
     9061 attttttgct gcacgttgga ggtattttac attaatctcg tcgtcgtgga tgccgccgtc
     9121 aagcaaggtc agtacatatc cctgaatggt tgtaatgggg gttttcagtt cgtgggaaac
     9181 atttccgata aattcacgtc gaaattcttc gttttttttg agtacttcaa tttcattggc
     9241 tttgtcacgt gtccattgtt caacatcgcg gctgacattt tcgagtacat cgcctttttc
     9301 catctggatt tttttgtcgc ttttacccag tttagtgctt cggatcgact tatagataag
     9361 ccgtatttta ttgtagataa atctgttcag agtatataac agagaaataa aaaccacaat
     9421 gaaagttgtg aaagccagtg tcagagctac tatccatttt gatgcagtgg ttagttcaag
     9481 tacagtcaga ctgattgcaa aaacaatcaa agccagaatg acagcaataa aaatggagag
     9541 atgacgcggg tttccggggg ttattttcat ttcttgattt caaaagtata tccaacgcct
     9601 tttactgtcc gtattagact taatccgatt ttttcgcgga ttttgcgaat atgaacatca
     9661 atggttcggt cgccaacaac aatgtcgttt ccccagactt tggagaaaat ttcttcacgt
     9721 gtaaaaactt ttcccggttt gctggcaaga agcgccagta gttcaaattc ttttctcgga
     9781 agaatgattt cctgcccttc gcgtataaca atgtagcgct caaaattaat gacaagccct
     9841 tcaaattcca gctctcgtga tgtatcgggg ttgatatcct ttgaaaaacg cctgaggtat
     9901 gttttgactt ttgtcagaaa cacctttggg gtgatgggtt tgcttataaa gtcgtcgccg
     9961 cccgattcaa gagcagcaat ttgcgcgaag tcctcattgc gtgctgttag aaaaatgata
    10021 accgactgct ttaaggactc atgttttcgc atctcctggc aagctgtaat gccatccatt
    10081 ccaggcatca tgatgtcaag aacaatcaat tgcggctcaa tatccaatgc tttgtttatg
    10141 gccgactgac catcacgggc tgtataaaca tcatagccct ctttttttag gttatagctc
    10201 atgatttcga gaatgtcctt ctcgtcgtca accagcagaa ttttaatgtt ttcgcctgaa
    10261 ttcattatgc aaaactaatt gttacacaaa gttattaaaa taccggaaga gtggcagtaa
    10321 gttacaattg cttaatgtaa aagtaataaa taatgaatac cgaactctga acatcgaacg
    10381 aggaagtatg aaggaatttt gaatgctgaa caaggaactt gtctatcgcc agacaagtaa
    10441 taaaggcgga aggacgaaat cttttggaac accgaataat gaagtttgaa tttcagtttt
    10501 acttcagtgt tcttacttcc ttgttcagtg ttcgatgttc tacagcccgc cttcaccgcg
    10561 gtaaagcgag agtttcccgt tgaggttgat tttaaggact gtcatatatt tatcttgaac
    10621 accttcggga acattgatgt aaacaattcc cggaactttg ctccacgaaa tttttccaac
    10681 tacattgaat ggaaccggcg tgttggatcc aataacttca atgttttgta tttcattgtt
    10741 caatcctttt agaatcacgt tgccgctttg ttttccctga agaaaaagat aaatggctgt
    10801 tgaatcttgt gcaatggtgg ttgggccgta aaagtgcccg agcggtaatc ctgccattga
    10861 ttcaaaaact gcttcatggt gttttgcatt ccattgtccc agttcttcta aaataaatgt
    10921 ttgttcttcc ggtatggatc catcggcttt ggggccgata tcaagcagaa gattccctcc
    10981 aaaactaatg caatcagcga aaatgctgat gatttcgtat ggagttttgt aggcggtatc
    11041 tgtgatgtaa tagccccacg atccattcat ggtcatgcaa agttcccagt tttctttttc
    11101 cggtctgatg attggaaaat gttgttcagg agtggcgtag tcgccatacc cctgcagtcg
    11161 tccattgatg attgttttgg gattgttttt caataccatt tcgcgaattc cggctgcatt
    11221 ccattctttg gcactgtgtt cccaatcgcc gtcaaaccat ataaggtcag gattgtataa
    11281 tttaagaatt tccgaaagct gaccgttatt gaatttcatg aatttttccc atcgctttgg
    11341 ctcatcatta atttcatatc tcgaagaatc ccgaagaaat cccgtataat gatcgtttgt
    11401 ccaatcaatt agtgaataat acaatccaag tttcaagtct gtttttctga tttcatttac
    11461 aaaaggagtg agaacatcgc gtttggctgg cgaacttttt tttgtgctca ggtcgttgag
    11521 tttggtgtcc cagagtgcaa ctccgtcgtg gtgtttggaa gtgattacaa catagcgtgc
    11581 tccgcttttc tgaatcagtg taacccattc ggctggattg tacttctcag ctgtgaattc
    11641 atttatttgt gccatatagt ctttatgact ggtgtggccg ttgtaaaatt cccacgattc
    11701 gcttccccgt ccggttgaat aaattcccca gtgaatgaag atgccaagtt tggcattggt
    11761 aaaccattgc atttttgctg tatgcgaagt atcggtctgg cctttggtat taagaaatgc
    11821 aaagacaagc agcgaaataa aacaaagctt tttcatatgg ttattgtatt gcagcaaaga
    11881 aacaaaacaa aacaaggttt cgcaacatta ttttgagatg ctgtacaatc cttatttgaa
    11941 gtggcaatca aatgagagat ggattttatt caaatgaata tccgaaatcg atttttggcg
    12001 ttgttttgtg acagttaatg aagttctgca gtttcttgaa ccattcaatt actgttgtgc
    12061 agttaacgat gtcggaacgg ttatttaaaa catcgttata ggatgaatat ggccatcctt
    12121 cagttttatt tgcaaagccg tgataaaccg gattgttgtt gatgtaagtg attagattga
    12181 tcaggtagtt ggtcgaatct acttcttttc gctggaagtt tttgatgaat atcccaccca
    12241 ttcgtttgaa ctgcttattg aaggcctgtg tgtaactgga aaacaaatca ccaagctgtc
    12301 ggctgatcag ttgtccgatg aataggtctg atgtggcgta atctgatggc ggaatgtttg
    12361 caagctggta atatttacgg agaaatttgc cgtacgtttt aatgcgtgat gacggggcaa
    12421 ttttttcaag ccggaaaaaa tccggaaggt cttttacctc caacttatcc aacttatcca
    12481 actttgccga agttccaaac ttcggcaaag tttcaggata agttccagga tgtaaagttg
    12541 gaggggtgag gattttttcc atactttcca gcatctcttc ccggcttttc acgcggatca
    12601 gcagatgaaa atggttgggc atcagacaat aggcgtaagt gtcgataaac ggcagcaggt
    12661 gaaaccggta tttctggata aaaaacaaat agttttcatt acagcggaaa acattttcaa
    12721 acccgtttgc atgattgtaa atatggtaat atttgtcagg ttccagatac attcatcaaa
    12781 tgtttatccg gtttgtcaga actggatata aataaacggc atggcgtcga ggctgtaaag
    12841 ctgatagagt aatattataa gaacggccag ggttgcggct ttgagtgacc aatgaacttt
    12901 ggtgaatcca cccagcattc ctgcatacaa ttttactggc agatattgca aaattaatcc
    12961 gcccagcatc agtagaaaag gagtggtata tatgctgagc cattgaggga acattgaaaa
    13021 atcagtggtc agcataaaat tataaaattc gcccgctttg acaagattgg ggactttcag
    13081 taatacagca gtgaaaacaa gaaagtgaaa agtaataaaa atgctgataa aagagtaaat
    13141 gcttttcggc atccattgct ggattttatt tctggttgat tggcttatca catcccaggc
    13201 gatagctgct ccatgcaaaa gtccccaggc aacaaaagtg agtgcagctc cgtgccagag
    13261 acccgaaaca aagaaggtga taaaaacggc cagcagaatc attgtttttc cgccccggcg
    13321 ccatgcaaaa gcgagtggct gatataaata ttcgttgagc cagttatata atgtaatgtg
    13381 ccatcgtcgc cagaattcgg tgatgttgcc ggccagaaat ggtttgttga agttgtgttc
    13441 aatgctaaat cccatcagac atccaaaacc aaccatcatg tcggtgtagc cggcaaaatc
    13501 gaaatataac tgtaacatgc ctccataagc tgccattaat gcatcgattc ccgtaaaata
    13561 ttgcgggctt tcaaatacac gatcaaccag attggcggcc agataatcgg cgaaaacaat
    13621 ttttttgata agcccgagca taatcagaaa aagacctttg cttagtatgt ttttatctat
    13681 gacgggtttt gaaataaact gaggcagcag gtcgcgcgct tttgaaatgg ggccagccag
    13741 tatgttcggg aaaaatgaaa tgtacaggac atagcgaaac cagtttcgtt caggttcttc
    13801 aattacctcg cgatgaatat caaaaatata ggtgagtgat ttaaaaacaa aatatgaaat
    13861 tcccaatggc tgcacaattt tcaaaatcaa tggttctgtc ccaggatgtg ctatacctgt
    13921 ccatgtttca atcagaaagt ttgtgtattt gaaatatatg agagcgccga cattcataaa
    13981 tacagacagc accaagagcc atttttttcc acttgtggtt tttgttttat aaattccttt
    14041 tccaatgaag aaatcaatgg tggccatcag aatcattaca acgacaaaga atccgctgat
    14101 tttgtaataa aaatacaaac tgaatatcag gagtactgcg ttgcgaaggc tgcggttgtt
    14161 gaaggagagt gcatacagca gataaaaaaa ggcaaaaaga ataaaaaatg ttactgactg
    14221 aaaaataagt ggctgccccg gcagatattg aagtgaataa agaaaatcgg taaacgaaaa
    14281 attcatatta cttaatgctt agtttttgtc cgacatttaa tttatcagaa gtcagcttgt
    14341 tgtccttttt tattttttca acggtagtgt tgtatttttt tgctatcgac catagtgatt
    14401 caccgctttt gacaatgtgc agggttcctg ttttggtggt ttgtgtggtg gtgttttgtt
    14461 tttgtgtggt cgtctgagtt tggggtacag tgcctgaagc gtaaattaca agcgttttcc
    14521 ctgcaacgat attgtttgaa ttcaatccgt tccatttttt tatttcatca acgcttactt
    14581 tgtattttaa agcaatggaa ctgaggttgt ctccttttct tatagtgtac acgatttttt
    14641 tgctgccaat tgttgtttgc tgtgacgatg tttgattgat ggttttttgc gttgtattgc
    14701 tggcggcaga tttgcttaca agtagcttct ggcctgcgtt tatgcgtgtt gttgaaagat
    14761 tgttccatcg ttgcagatcg gcaacggtga ctccatatcg ctttgcaatt gagcttagat
    14821 tatcacccga cctaactata taatattgtc ctgttccggt gttggaatac ttcgtgcctc
    14881 cggtttggtt tttataattg tttattttat cagtagtggc aatttcctgc cggttgtaat
    14941 aggtggattt gttgatgacc catttcccga aattggttgt gtcaatggca ttgttgaaaa
    15001 caagcagact gtctggtttg cgcgataaat aggcaatata ggaattgaga agggcgttga
    15061 aatataattc tcctttcact ttatatcctt tataactcag atgacaacgg tcgcgctgtg
    15121 aaagtccgtt ttttgcccag tttagcatgg aacgcggtcc cccagagatg ttgaagtagt
    15181 catagagtac aacattctgt tcttttgaaa gcaatctggt gagggtactg taatctttgc
    15241 aattggtaat gttgcggccg cgataataaa tgtcctgagc attgggaagc atgaaaacag
    15301 cattgggggc tgattgccgg aatagtgcaa tggccttgtt gatgctgctc atgatgtatt
    15361 gatagttgaa agtacctctg aagaagtcgt tgatgccgag gtcgaaaatg acgagatccg
    15421 gctttattgc tttcagttgt gcagcggtaa gattctgatt gaaaaaactg tgatatcctg
    15481 caccattgat tcctacactg atgtactgaa ttcccgtatt tgccacactt tgaatttcta
    15541 tgccatagca ttcaaaatat ttttgttcgg gggatgtttt gtttacaacc aatgtgagcg
    15601 tatcagatgc tttgggcaga ataacttcga catatggaag tgtgtcggaa tgacaatcag
    15661 catcaaccca ggtgtttgaa ccaccagctt tgattttcgc gtcaaagctt tctttgcctt
    15721 tgtgacaata gatgcgtatg cgtttgaaat cgggttgaat ggagtaataa tgctttctga
    15781 aaatcagctt gaatcctgcg gttgagtcgg ttgtaaaaac agtgatacca ctcacaccaa
    15841 tgttcatttt tggtttaagt tcaacatttt tcgaggccga ccatgtgccg tgtgcctgtg
    15901 caaaataatc aaaggccgaa tgagtgccgg ctgcctgata tgggaaaaca atgccgcggc
    15961 cgccaaaacc aaaaatctgt tgcagcatcg cacgggtgat gcctgttcca atgtcgctct
    16021 gaatgtgaga atcgcctata tgaagaatga caactttttc tttatttgcc tttttcagtt
    16081 tttcaaaaaa cggtgcaatg gcatcgatct gataccattc aaacatgttt ttatcatatt
    16141 gcagaaaatc ataaaattcg ctgtatttga cggcttgctt caaataattt gtgtcagcag
    16201 gatttttctg ggcatggctt tcaaggccga atagcagaag tccggttaac aaaacaaata
    16261 ttttcaatgc gtttttaatc attttccggc agttgttccg tgaatgagaa atttattgta
    16321 ttcgcccatg atggcaagga aaagctcatt gacaacaatt tcgcgacctt tgtcgctgta
    16381 atgaccgtcg cgcgaggccc atcctttttt gttccatgcc agcaccgaat tttctccgcc
    16441 catcagttga taaagatcga aaaatgcgca accgtttttc agcgccactt ctttctgaac
    16501 atcacggatg cggcttacca tcggatacga tgcatatgtt ccgccaacgc tgcgtgcaac
    16561 atcgtttacc ccaatcacca gaacagatgc atctggcatg gctttacgaa aatgcgcata
    16621 caatctatcg tagctgtttt ttatggtgtt gagtgatgct tcgtttttta cgtagggcgt
    16681 tacatttccg ccaaactgca aaatgacaag tttgttgttt agtttaatga attgggcttt
    16741 gagaaaatca ttctgtatgt tcatcaaacc atcgccgctg tgtccccgga gtccgtaatt
    16801 gtcgaattga actcctgaca tgggatcaaa actgagtccg taaatttcgg gactggggcc
    16861 cgtaaaaacg aattttaaac ttaccggatt tgatttcagt ggcagtttaa caacgttaaa
    16921 aggatcaggg ttgtcaagta caacttcggc caaaagctca ttgttttttg agttatacac
    16981 tttgagttct gctggttctg ttgcttttcc ataccacaat ctggcaatac tgtaaccgcg
    17041 gaatagtttc agaaaaacag ttgcatttgc ataggaacag gcaggaacct taattttaat
    17101 ggtgtcgcca ttttcgccaa tactgtctcg ggtgctttca ggtgtcgaaa accgatagcg
    17161 gaaaacggtt ccgccagggc agtacaaccc tttggcagag cgattgtgga acactgtata
    17221 acgaacccag ttggggcttg tgcttcttgt ataactcaca ggagaagtta catcatctat
    17281 tggcacatag ccaactccgc tgcctccgaa tttatcctga aacatgatcc gaagcaagct
    17341 ggtaatgcgg tctccttcaa tttgtgaatc accgtaatga ccaatgcgga tcaatgcaga
    17401 atcacttttt tgcaggtatt tgaaaaagtt ttcaagggcc gtgctaccat tgcttccagc
    17461 cgggttgatg aaaaaatcgc ttgaactgaa aactgattca tccagaatac tcatgcgctt
    17521 gatggcgtcc tcattcaggc cataggcgcg cgattcgagc atggcgataa cactgtcaac
    17581 attttgagtg ttgtctttaa ctttgaaatt caaaatctct tctttcggaa taaacgtaat
    17641 cactttttcc ccgagaacaa tgttgtcttt cggccagaag caggatgcaa ccagcaataa
    17701 tacgcccgtt agtacaataa tcgaaaaaac ctgatatctt ttcattttaa acgttcaact
    17761 tttcgttgat gatggtcagc atgtcaccga catttttcat tttaaccatt tctttcagtt
    17821 tgaaacgaaa accgaaagtt tcttccactt ttgcaatcat agtaaggtgt gtcagactgt
    17881 cccatttatc cacgtcgtta gcggtcatat cgggattgat aatcagggct ggtgcctgaa
    17941 aaatatctct gaaaatttcc tgaacttttg tgttgatttc ttcgttgttc atggtgtttt
    18001 atcctttcat taaattcatt atgtctgttt gcatcagaac cattccggtt cctgggaaat
    18061 aatggagaac atccggatca tcgcattgtg tgcggtcttt aatgttaaat cctatttttt
    18121 cgtaggtgtg aatggccgtt ttgttttcga tataggtcat cagttcggcc tgactgaccg
    18181 gatactgctg cagcagaaat tttgcaacat gcgctttgat aagttttgcc gcaagccctt
    18241 tgccccggtg tttatccgaa acaaaaactg attctatctg catggttcct tcggtgcgat
    18301 gaaccatcat tttatcggtg atgtgttttt ttgattgcac aaaatccagc gcgccttttg
    18361 gatagtaatg ctgcatcagc gccgaacgcg ccatccacga tgaaacgccg tcgagacctt
    18421 caatccatgc cgaaacacag gcaacgggtt ctccgttaaa ttcagcaacc agaaaatgaa
    18481 atggcgaaaa ctcacatcct tccacttctt cgggcatcat ggcggtaatg gcatcgcgcg
    18541 cctgctcata gctgaggcca aaaagtgctg cataacttgt attggtgccc gttgtaccaa
    18601 tgtcggcctt cagaatggct tcggccagaa atgcataatc gctttccttt gcacggcgta
    18661 tgatgtagtt aagttcagtc atgaccaagt atcagattat tgatttctct gcgatttatt
    18721 ttgccattgt tgttgagcgg aaaggcgctt atgaagcgga attcggacgg gatcatgtag
    18781 aacggcatct tgcttttcag atattcgccc agttcatgca ccgcgtcttc gctgccttca
    18841 atggctgcta caataacgtc atttccagta gtatcgtgtg cggccatggc aacaaccctc
    18901 cggttttcgt aaaacgaagc aatatgatgt tcaatttcac tcaattccac acggaagcct
    18961 cggattttca cctggtaatc cttgcgtcca agatagaaat acagcccgtt ttcacgaatg
    19021 cagatatctc cggtgcggta aaatttcttc ccgtttactt caacaaatgc ctcgcggttt
    19081 ttttcttcat ttttcagata gccggcaatg acctggccgc cgctgaggag cagttcgcct
    19141 ttttcgccat cgctcagaat tttattttca tcatccacaa tcacggcttc tatttccagt
    19201 gtgggccagc caatgctgac aatcccatta cgcgacggaa tatcattttc aggcatgcga
    19261 taccaaacgg aataaatggt ggcttcggta ggtccgtaca aattttggaa ctccgcattg
    19321 ggacagcaat ttttccattc cagggtgagt ccgtgcggaa cagcttctcc gctgaggcac
    19381 atgtatttca ttttgtccag cataatctca tcgaagtacg gccgcagata agccaccaca
    19441 ctgggaacaa gaatggcaaa ttcgatttcg tattcttcga gcagtctgaa aattgttggg
    19501 tatttaatgt tgttgttcgg aactgagaaa agagttcccc tgaaaataca cggcgacaga
    19561 atagaaacta tgctgaggtc aaaactgaga tcgaacattt gcagaaagcg gctgcccgga
    19621 cccagatgat aattcatggc ttcgaggttg cgcagaaaag agcgcacatt gccatacgaa
    19681 accggaacgc ctttgggctg gcctgtgctg cccgaagtaa agagaatgta ggcaacatca
    19741 ttattggccg gaacagcagg tgcattgaat gtactctctt tcaaatcgca tgtgcagaca
    19801 aatgttgctg atgcttccag tttttcggca ctgtgtaaaa taagttgtat ttcggcttgt
    19861 tcaataatgc tggcaatgcg ctcggccgga tggtagggat tgagcggtac ataagtgttt
    19921 ccggtagccc agattgccat ggctgcagca taagtatcaa tatggtcgga tgcaacaaca
    19981 ccgatggcag cttttttata accacgcgaa gcaatttcag ccgcaatggc atcgatacgg
    20041 ctccggaaag ccgtatatga atgaaataca tcttcaatgc aaatggcatt caggttgtcg
    20101 tcggattctg gaagaaaagg aaatatcacc ttgtctgttt tttgcaaaat tagtcatttt
    20161 aggcaatatt tttgcggttc tgcgcgctat tcattgacat cctattgtta atcatgcgaa
    20221 cgttagccct tttattcatc atttttattg ttggtccgat gctgcaggca cagaccgaca
    20281 ccagtctgcg tgccatgaat gagcgtgaga aagaattata caaactgcta atggattatc
    20341 ggtcaaaaaa gtggctgaaa tcaattccca tctcgccatc actcaataaa gtggcccagt
    20401 gccatgtgcg tgacctgaat acatatccgc gcgcaaaaaa atgcaatatg cacagttgga
    20461 gcaaaaacgg accatggaag ccggtttgtt ataccgataa tcataaaggt gccgatctgg
    20521 cgcatatcaa accatcggaa cttagctctt acaccggaga aggatttgaa attgcttatt
    20581 ggaattcaag ttctgatttc actgcaaaga atgcattgga cgggtggaag tcaagctatg
    20641 gacacaattc gatgataatc aatcttggaa tgtggaaaaa atcgaagtgg agagccatcg
    20701 gtattgctgt tgaaggcaat tatgccgtgg tgtggtttgg caaagagaca gatccagctg
    20761 gagaaatcat aatcaagtaa tgtgtttaca acatcgaccg gatcagcaga aacaatccgc
    20821 ccgacatggc tatggtaacc ggaatggtaa tgacccaggt gagcagaatg ttgtaaatgg
    20881 ttttcatctg cagatttttc catccgccct gatacaccat ggagcccgca ataccacttg
    20941 aaagcacgtg ggtggtactc accggaagtc cgatggttga tgaagacata atggttgccg
    21001 tagctactat ctgtgcgctg gcgccttgtg cgtagctcat ctgcgacttg ccgatttttt
    21061 cgcccagtgt tgtcacaatg cgcttccagc caatcatggt gccgagtcca agcgacaaag
    21121 aaaccatcag gattacccac cacggtgcat attcggtata ggagtccatt tctttaatgt
    21181 cgttcttcag catcttgctt tccttgtgat tcaaaacaag cgctttgttt tttactaccg
    21241 atgggctgtt cagaaattca ttcgtttttt tcttgatggt cagaatatct tttctggcat
    21301 tgaaacgatc gagtttgtca aattcgtccg ggttttgtgc aatcagcaaa ttctctttca
    21361 gatctgcaat acttgtcgtc agcgcaatgt atccgggcat gtcggcggcc agaatttttg
    21421 tagaatcggt tttgccaatg taatattcaa tgtgatttac attgccgatc atattggacg
    21481 gattttttgt gttgtcgagc gcaaagtgag ccggcagcag acttatcaga atgatcatga
    21541 tgagaccaac acctttctgg ccatcgttgg aaccatgcga ataactaaca gtggtgcagg
    21601 ttccgataag cagcaggcga acccagaaag gaggtttttt ttcaggatcg gcggctttga
    21661 aaaacttttt cttccggatg gttcttctga aaatatacat taaaagtatg gtgagcccaa
    21721 agccgatgac aggcgagatg agaagtgcac tgagggcttc aatgactttt gtccagttga
    21781 gtgcggcacc actcgaatca gaaatgaata agtaggccag gccaacgccg aaaatgctac
    21841 cgatcagcgt atgagagctg gagcagggaa taccgaaata ccatgtaccc agattccaga
    21901 taatggctgt cagaatcagc gatgcaacca ttgcaatgtt atggctgatg ttctggtcaa
    21961 gcagaatttc aagcggcaac aggttgatga ttccgatggc aactgcgatt ccgccaaaat
    22021 acactccgag gaaattgaag ataccggacc agattaccgc aacgcggggc ttgagcgaac
    22081 gtgtgtaaat tacggtggct actgcgtttg ctgtgtcatg aaagccattg atgaattcaa
    22141 aagccatggc tgccagtatg cagccgaata acaaaagggt catcccaaga tctagtccga
    22201 acatttctga aaatttccgg caaatttctg gcaaattcca atatgaattt aatgttacgc
    22261 attaataacc taatggtaac aataaattaa tacagggctt gctgaaaaac acgtaacact
    22321 cacagaagca atgacatatt cttatttatt tgcagcgtga atgcaaggac ttttcgggat
    22381 ttcatcaaaa atccctgcat ttgaaaatga aaacgataaa acagcataaa atatatgagc
    22441 ttgcgaatct gaattaacaa ttttcttaaa gcatagatta ttgctgagag tgtttgttag
    22501 cctgatgtac gccatcttct tcgttgcctt cgactcgaag tattactata catcttcgtc
    22561 ttgtcgcctc aaatatggcg cacctcagac tttttcagca agccctgata tcggcctgtg
    22621 atccatgcta agtcacaggc caatacaatg catatcatat gcagctttcc gctagtcaat
    22681 caaccggtaa tccgcttcag caacctgact tttggtgcgg atatcgagct cggtcagttc
    22741 attcacaaag cgggtgatgt tctgaatttc agttgtgata ttgaagaaca gcagacttga
    22801 acgggaagcg ttttccttac gttttacgcg ttcgatctgg gcacggcgga caatttttat
    22861 tttatcgcaa acgacattgg ctttttcgtg cagattatcg gccagtccgc gaccggaatt
    22921 ttcgagtata atttgtttcg aaaaatcgca caattgctgc agatcttcgg agagtgactc
    22981 gaaatcgcgc atctggtttt tacccaggtt tttgtgctga ttgttgatgt gattatatac
    23041 cgggtgtgtg agatagccaa gactgacagc gatttcgcgg aggatttcgg catttttcag
    23101 atagtactcg ccatataaaa catagttgtc tccaagagcc gaaatggagc tgccgatcgt
    23161 ggaactgaac aactcggtac ggctgtttaa atcttttaca atttcgtcgg cttctttcag
    23221 ctttttcagt ttttcttctt caagtccttc gcggatcagc cttaccactt ctggaatttt
    23281 ttcaattacg aaagaaacat tgtttctgca caggtccaga atttcctgtt cgcccgtgtt
    23341 ttcatctatg cgcagcatgt tttctttttc ctgatattct ttttcgcgac ggcggtgaat
    23401 gactttgctg cgtataagaa caaatactgt gagtgcagaa agcagaatta cagcaacgat
    23461 actgccaaga taaagaacat aggcaagcaa tgcagccgag agaaaagcaa ggaatgcagt
    23521 aataaaccaa cctccgataa ccgttgttac tcctgatacg cggtacaccg cactgtcgcg
    23581 gtcccatgcg cgatcggcca gcgaagttcc catggccacc ataaaagtga cgtaagtagt
    23641 tgaaagcggg agtttgagcg atgttccgaa agcaattaaa ctggatccaa cggtgagatt
    23701 aaccgccgcc cgcaccatat caaaggccgg tgcatccgga ttattactca tgggcggagc
    23761 tgtaaatcgt ttttcaatcc agcgatgaac agctggaatg cgtaccagtg taaaaaagtt
    23821 accaatagca agagctaatt tcacgagtga tcgggcaaca ggattggtgt caaaattttc
    23881 tacaccttcg ttttggcgcg ccaatccaac ttcggtgcga ataactgttc tggttttttt
    23941 attgaaccac aatgtaagaa gcatgataaa acctgctccc atcagaaatc caacaggcac
    24001 ctttgccgga gcgcttaaag attccatggt catggatgga tctccggtgg cagcataagc
    24061 attgtatgca tcgagtcctg cgagcgatgg cccgagaaag ttcaccaagt cgtttccggc
    24121 aaaagccatg gcaatggcaa atgttccgta aattacgata acccggaaaa cattgactct
    24181 gaaaaggtat tgaagaataa gtagcaatgc tgctgtacca gctgttatga aagacaatat
    24241 catgagaaga tttgcagata catattcttt catcggttta tcaatgaaag aagctgattt
    24301 cataccttta aaaaatataa aatatgaaat gagtgctatg gcaagaccgc cgaaaatggc
    24361 gccgccgatt ttataacgag aaaaattgaa actgaatact gtacgtgcta ttttctgaat
    24421 taataatcca aagaaaaatg caacggctat ggaaacaaaa atggcaatta tcattgcaaa
    24481 ggccttttct gtattcatat acatatcaat atcggtgaag gcctgaccgt tttcaatcaa
    24541 cttggcaact gcaaaagaga aagatgcccc caataatgct gcgaccagtg aaacggtagt
    24601 ggaggttggc aatccaagag tgttgaagaa atcaatcagc agcacatcgt tgagcatgac
    24661 cgcaagataa atcataatga cttcttcgaa catgaaatat tgcggatgaa aaactccgga
    24721 tcgagccaca tccatcatgc cgccggcaaa caatgcgccg ataaaaacac ctgccgatgc
    24781 aaccaccagc aaccatttga atgtgagact tcgggaacca tatcctgagt tgagaaagtt
    24841 tactgcatca ttggccacac caacagtgag atccatgatt gaaagcagaa aaaggatcac
    24901 gagtacacct ataaaataat attccattgt gagaaagatt aatgatgtaa aattaaagat
    24961 acaacaaaaa ctctgtatta gggctatgtt aagaaattat taggtgcgat gaaatccctc
    25021 agagtgtttc aatccccatc gagtgatttc agcaacaaaa aacgggttta gtttttacgc
    25081 tgaacccgtt tttaaatatt agataaatac agtattacat gcctctcagg ttggctttac
    25141 gcacgctgtc tttttgcaaa ttggtttgtt tgaaattgta atttagtact gcaccttccc
    25201 agtcaccata catgggattt ggcagaacaa taaagcgttt gccgaattct gatttatttt
    25261 gatcagttac atcaaaacga tccgatactg atttgttttc aaaaataccg ctaaaatcgt
    25321 tcatgttatc tccaatcagc agaataattt catgagtttg cgaaaccttg ttgcggcgtt
    25381 cgtttttgct gctggtttta tcgcgcagta aaagatgatc ttcatcaatc atcggaaaac
    25441 caagtgcgcg catgttttta acagtggcat ccttttccgc aattttgcgg ttgctgacat
    25501 aatacacatc gactcctttt gaaacagcgt atttcatgaa ttcaacggcg cctggaatgg
    25561 cagtggcaga ggccttttcg atccattcgc cccagcgttc cggatatccg aaattgccga
    25621 gaacattctg tgcttcgtat ggactgttgt caaccatggt ctcgtcaata tcaacaacaa
    25681 ccgcgagttt tttggtgcct ttgtatgaag caacagtctg atcaagtgaa atccgtgcag
    25741 tattgaatgc ctgatagcac aatgcgcgca tttcggccga tgtctgatac cagagcaccg
    25801 ccataagcat gtaatcgtaa tcaaaattgt ttacataaac agtgtcgtga acaacaaccg
    25861 ttttttcaac aattttctct gtctggcatg cagacaaggc gagaaggcca actaaaacaa
    25921 taacgaacag atttttcata ggttttcgtt ttgattaaaa aggccggaca aagccggccc
    25981 ttatgaattg ggttgattta ttaatcagca agttttttat acttgttgta gcgccatttt
    26041 gcattgtctt cgcaggcagc aaacaacacc ttcgcttctt ccgggaaggc ctgaacaaga
    26101 gaggtgaagc ggacttcgct cttgaggaat tcctggaatt tgctccagtc gggctctttc
    26161 gaatcgagta tgaacggatt tttgccttct gcttcgagct gcggattgaa acggtagttg
    26221 tgccagtagc ccgattccac tgcaaatttg ccctgagcct gggttttgcc catacctgaa
    26281 cgcaaaccgt ggttgataca gggagcatat gcaataatga gtgatggtcc cggataagct
    26341 tcagcttcgc ggatggcttt cagatactgg ctctgatcgg cgcccatcga aacctgtgca
    26401 acatatacgt aaccatatga catggccatc attccgagat ctttcttgcg ggttttcttt
    26461 cctgaagcgg caaacttggc aacagcaccg gtcggtgatg atttcgatgc ctgtccgccg
    26521 gtattggaat aaacctcggt atcgagaaca agcacattga tgtcttcacc gctggcaagc
    26581 acgtggtcga gtccgccgta gccgatatca taggcccagc cgtcgccgcc gaatacccaa
    26641 accgatttct tgatcaggta ttgtttcttg tcaacgattt cttttgcaag ttctgaattg
    26701 tctttttcag ctaatgcaag aagttttgca gatgctgttt cagatacttc agcattgtca
    26761 atggcagcca tccattcttc gcatgcggct ttcaggtctc cgcccaagcc agctgcaatg
    26821 gcttccttca ttttcattgt aaggaattca cgcattttct taacgccggt ggcaatacct
    26881 aatccgtatt cagcattgtc ttcgaacagt gaattgcccc aggcaacgcc tttaccactc
    26941 ttgttgttga tggtgtaagg agttgaagga gctgaaccac cataaatgga tgaacatcct
    27001 gtggcatttg ctacaatcat tctctctcca taaagctgag tgatggcctt gatatacgga
    27061 gtttcaccac aaccggcaca agcgcctgag aattcgaaca atggttgtgc aaactggctg
    27121 tttttaaccg atttactctt ttcaagcacg gtgtctttat agccaacttt ttcgtccatg
    27181 taagtccagc gttctttttc agccagctgg gtttcgagcg gtttcaaaac aagcgctttg
    27241 gtttttgcag ggcaaacatc cacgcagttg ccgcaaccgg tacaatccat aacacttacc
    27301 tgcatgcgga agttgtattc ttttgttgca gcaaggcctt taagagtagt ggttccggca
    27361 ggagcagcag ccaattcagc ttcggtgaga aggaaaggac ggatggcagc gtgaggacat
    27421 acgtatgcgc actggttgca ctggatgcag ttttccgact gccattcagg taccgtaaca
    27481 gcaataccgc gtttttcaaa tttggtagtg cctgaaggga atgtgccatc ttcgcgaccg
    27541 acaaatgttg aaactttaag gtcatcaccg ctctgacgat tgataacatc agccacattg
    27601 cggatgaagt caggatgtgt ttcatcttcg aaagctgttt tgccttcgag atttttccat
    27661 tcggcaggaa tggccacttc ggtcagttca ccaccacgat caacagctgc ataattcatt
    27721 ttcacaactt catcgccttt gcggccgaaa gatttgtcaa tgaatttttt catttccttc
    27781 acagcctgat cgtaaggaat cacacctgaa attttgaaga aagctgcctg catgatagtg
    27841 ttggtgcggt tacccagacc aatctcgtct gcaatttttg ttgcgttgat tgtgtagaat
    27901 ttgatttcat tcaccgccag gtatttcttc atgtcttcgg gcaggtgagc tttggtttct
    27961 tcaacatccc agatggaatt gaggaggaat actccgcctt ttttcaggcc tctgagcata
    28021 tcgtaacgac ccagataagc aggaacgtgg caagctacaa agtcaggtgt atttacgaga
    28081 taaggcgaac ggatgggaac atcgccaaaa cgaaggtgtg atgcggtgaa tccgccagat
    28141 ttttttgagt cgtatgcaaa ataaccctga caatatttgt tggtggtttc tccaatgatt
    28201 ttaatggagt ttttgtttgc gccaactgta ccatcagagc caagtccgta gaattttgcc
    28261 tggaatatgc tgggatccga aatgttgatt tcggcaatct gtggaagtga tttgaaggtt
    28321 acatcatcaa caataccaat agtgaagccg tttttcggct cagtgttttt cagattatca
    28381 tatactgaaa taatttgcgc aggtgtagta tctttcgatg aaagaccata acgtccacca
    28441 acaacgagcg gagcattggc tttgttgtag aaaagattct tgatgtcgag atacaaaggt
    28501 tcgccttcgc tgcctggttc tttggtgcgg tcgagaacgg caatgcgttt tactgatttc
    28561 gggaacactt tcatgaaata tttttcgctg aaaggacgat acaaatgaca agtgagcaaa
    28621 ccaacctttt cgcctttggc attgagataa tcgatggttt cgcggatggt ttccgttaca
    28681 gaacccatgg caatgatgat gttttcggca tcgggtgcac cataataggt gaacggatga
    28741 tattcgcgtc ctgtaatttt attaagctca accatgtatt cttcaacaat gtcggcaacc
    28801 ttgtcgtaga aaggattggc agcttcgcgt gattggaaat agatgtctgg attctgagct
    28861 gtaccgcggg ttacagggtg ttcggggttg agtgcgtttt gacggaaact ggcaagagct
    28921 ttcatatcaa ccagtgatct gacatcatca ttgtcgagat attccacttt ttgaatttcg
    28981 tgcgatgtgc ggaagccatc aaagaagtgg agaaaaggga tgcgtgaacg gattgatgca
    29041 aggtgcgcag caggcgcaat gtccatgatt tcctgtacag atcccgttgc cagcatggca
    29101 aaaccagtca tacgtgtact cattacatcg ctgtgatcgc cgaaaattga caatgcctgg
    29161 gctgcaattg cgcgggctga tacgtgaaaa acgcctggga gcagttcgcc cgaaatttta
    29221 tacatattgg gaatcatcag gagtaagccc tgagaagccg tgtaagttga ggtgaggaca
    29281 ccgctctgaa gtgaaccgtg aacagctccg gctgcaccag cttcgctctg catttcgact
    29341 acttttacag tgtctccgaa aatgtttttg cgtccgttgg ccgaccattc atccacatat
    29401 tctgccatcg tgctcgatgg tgtaatggga taaatcgcgg acacttcgct gaacatgtac
    29461 gatacatgcg ccgcagcgta gttaccgtcg catgtaatga actttttgtt tttcatgatt
    29521 atgtaagttt ctgtgtgttt gtttttagag cagcgaaatt aatcatattt tatttcttta
    29581 aggtgtttta tcgtttcttt ttgagaaaat ttaatctgag gagagctgat ttgccttaat
    29641 tgtttatgtg ttaataattt gtcggttgat acctcaaatt tgtatgtttg cgaaataaaa
    29701 atcatgggaa cacgttagtg tttcgtcaag ttaaaaatat atatcaatga aaaacctgat
    29761 tttttcgtta gtgttgttgt ttcctttttt ttcgcttttt gggcagactt ctgatgaaaa
    29821 gcccgctgta agaatttcgg acatcatggt tcaaagcgga tttcaaaatt tcaacgattt
    29881 tagtttgggg ttgaacgatt ttttgaaact cgcacctggt tcgccatggc tgcaaaatga
    29941 tttcagtgat tattccctgt cgcacgcttc aggtaatttt accggattgt tttcggtgtc
    30001 ggttgggttg aaatttgccg gcaaagaaag aaaatcctat agggcaaatc ctgtgttgcg
    30061 tctgggaata tttgatgtgc gctcttcgcc cgatggcgtt ttttacaata agcgagaatc
    30121 ttttccttat gatacattga cgtcgtctgc taccggcaat caatattatg ttgattcagt
    30181 ttcaaatatg caatatgaga tgcagtattc aacggaacat attggccttt cagcttctct
    30241 tcagtttggc accaatccgc aaaaccgcgt gtcgttttat gccggaacct cgcttgttgc
    30301 ggctttatcg gtcagtaatt caaccaaatt aagtaaagca gaatatgtgg ttttttcttc
    30361 agatgatctc tattactcat atcccgttta taacaattat gcctatgatt atgaatcggt
    30421 ttttattggc aataaatcat ccatgctgtt atccatggga ataccgatgg gcattgattt
    30481 ccgcttgtcg cgttcgcatc atttctggag tctgattcat ttggtttatg agttcagacc
    30541 ttcggtaaca atgacatcaa ttccagaact caaaaattat gtaagcacag gaatgtgcaa
    30601 ctccatgggg tttaaggtta cctggtgatt tattgtttta tgtttgattg aatataccga
    30661 caggaattat attaatgtct aaacaactct gtctctatca atcattgtaa aaaccccacc
    30721 tgatgcagtc gtgctaaatt ttattgtttt aaaaaagcag ataattctgt ttaaaggcaa
    30781 atcaattctg tttctgaata aatgtatatt tgtgaagtca aaattttata gaaatggatc
    30841 gaattcaaat aaaagatcga ttattta
//
LOCUS       DAOPKE010000030        30328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1604_length_30328_cov_12.619430, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000030 DAOPKE010000000
VERSION     DAOPKE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_30328_cov_12.619430"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKY63_05670"
     CDS             <1..339
                     /locus_tag="PKY63_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPF00134.1"
                     /translation="TEREKWEKLTAKEKAAQLYSFDKPTKEITVTQEMRQKEFDNFKT
                     TDDHGLLITGLAKDQNGTIYFKVKNSWDAVGKYDGYFYVSKAFVMLKTTDMLVHRKGI
                     PPAILKKLKL"
     gene            518..1852
                     /locus_tag="PKY63_05675"
     CDS             518..1852
                     /locus_tag="PKY63_05675"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPF00135.1"
                     /translation="MKNILILGAGLSASTLIRYLLQKGEQNQWTVTVADMDIETAKKK
                     TDGYKNGKAVSFNIKDEKNLAELIMQNDLIVSMMPALFHPVVAQQCLNFSKHLFTASY
                     VSPEMKSFNEEATAKNLIFFNECGVDPGIDHMSAMKVIDEIRAKGGRIIGFESNCGGL
                     VAPEFDNNPWNYKFTWNARNVILAGQAGARFLHNGKYKYIPYTRLYERIDKTSVLNFG
                     NFEIYANRDSLSYKEVYGLENVETIVRGTMRRPGYCAAWNCFVQLGVCDDTYELEHPE
                     KLTNRSFIESFLPENPGLSVEDNFCQYLKLDRNGEIFKKMEWLGLFTKTPVGTGFKTP
                     AQILQSIIEPKWQLDKNDLDLLVMQHTFDYELNGKKYRRMSSMGIIGTDTVHTAMAIT
                     VGMPLALAIELFLTGKIHGKGVQIPVKPEYYNPILEGLKPFGVEFFEEETEI"
     gene            complement(2104..3735)
                     /locus_tag="PKY63_05680"
     CDS             complement(2104..3735)
                     /locus_tag="PKY63_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00136.1"
                     /translation="MRKILLHIFLFLVSFSAIAQFEEIDKRILSARGKTNSEPLQALR
                     EAEMLIDECSHLNYEKGLAECYNLQGIVLFTRGLNDMALQSFIRSLEYYQSVDDTVGI
                     SLVYNNLGVVSYTISKYHYSIFFFNQSLKIQIRAKKFRNIVDLKNNIGSLYEKLRMYD
                     TAMALHRESILISRQHDYPVGIATAMNNLAVIYENTGNRDSAVFYYKASLAFHDSIAK
                     SQLALTYSNLARTLLNGGDSKSAAEYLDSALFNAIESDASAQLPEIYKLYSQYYESTG
                     EIAKAYENLKKYKDITEKIESQTTEGDFADFILSMQQSKWQKEKSLLDKQMKLQNHVQ
                     WLIIAIIAVALVLFVLLFINIRNRNRMLDQRHALAESEKRRLSEELENKAIIAQLEKE
                     KLTNDLQMKERQLTSMTMHIVTKNETLQEIEKNIDQFVSANTDKGNDASLKKIQSIIR
                     MNAGDEAVWNNYFYHFDQVYPGFFKVLSETHPDITQGEQKLCAYILINLSNKEIAHVM
                     GISEASIKIKKNRLSKKLKLDIASDLTAYLRKFAE"
     gene            complement(3745..4842)
                     /locus_tag="PKY63_05685"
     CDS             complement(3745..4842)
                     /locus_tag="PKY63_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF00137.1"
                     /translation="MGYTSLGYIILMALVAVFGYFIIPDATSDANSQQVEIALQKPGF
                     ECRFLEIPIASSEKVSFFEKLLYGEPLSVSSKAIFSYYFRKDSLYIETYTGLTPNSGQ
                     IEVYDCRKLQTAWEKRNGVIRVSSGIELEKFITSKMIVKKKFLLGTDRFGRDYFSRIV
                     LGARVSITVGLVAVFIALLIGIMLGAIAGYYGGIIDRFLVWLFSVVWSLPTILLVIAI
                     TFALGKGFWQIFVAIGLTFWVDIARVVRGQVMSIKQRDYIEAAQALGIKNRKIIFRHI
                     LPGIRGPVIVLAASSFSSAIMTEAGLSFLGIGVQPPVATWGGMVKEYYSHLVLDSAYL
                     ALLPGLAIVLAVMAFMILGNELRDNLDVKNA"
     gene            5002..5847
                     /locus_tag="PKY63_05690"
     CDS             5002..5847
                     /locus_tag="PKY63_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00138.1"
                     /translation="MKTLMTISLIALFVNLFAQDIPVATAEQFKRFKESTTYLVKYDD
                     PFSSFNEYITEDMSKVWKITPFKIISPDEFETLSKDKKNSFIFLSEIFKEEGEKTAKW
                     NLLNITLGSNAGSANMNSMPDLGSAPLNYVSDNEDNEEEYLYKLAGVLRFFQYYIQYN
                     MANPGSDIKSVVKNNQSKLTGKELWLLQSDMSDEVNSLEKISKYFSGTVKFVTPEEIR
                     SAIHAGNEKVVFLHQVTPGNSVGKWSLKFLLCASDGCPVYYDVTTVGAGKNGRFSASD
                     FKAIN"
     gene            5861..7534
                     /gene="ettA"
                     /locus_tag="PKY63_05695"
     CDS             5861..7534
                     /gene="ettA"
                     /locus_tag="PKY63_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPF00139.1"
                     /translation="MSDDKKIIFSMVGVNKIYPPQKQVLKNIYLSFYYGAKIGVLGLN
                     GSGKSTLLKIIAGKENPTEGDVVFSPGYSIGMLEQEPHLDNNKTVKQIVEEAVQPIVD
                     LLKEYEEVNNKFSEPMSDDEMNTLIIRQGELTELIEQHDAWELDSRLERAMDALNCPP
                     SDQIVKNLSGGERRRVALVRLLLQKPDILLLDEPTNHLDAESVQWLEKHLQEYQGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSARLAQEEKTESKRRKTLER
                     ELEWIRMAPKARHAKGKARLSAYEKLLSQDVKEKEQRLEIFIPDGPRLGNQVIEAKNI
                     SKAFDNKLLFENLSFTIPPNAIVGIIGPNGAGKTTLFRLMMGIDKPDSGDFTVGDTVK
                     LGYVDQAHSSIDSEKTVYQTISDGMENIRMGSREINARAYVSRFNFTGPDQEKKCGVL
                     SGGERNRLHLALTLKEEANVLLLDEPSNDIDINTLRALEEGIESFAGCAMVISHDRWF
                     LDRICTHILAFEGDASVVFFEGSFSEYEENRKKRQGDTPVRFKYKKLSV"
     gene            7603..8439
                     /locus_tag="PKY63_05700"
     CDS             7603..8439
                     /locus_tag="PKY63_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF00140.1"
                     /translation="MNEIVVGIDFSKGALNALKYASLLAKGFNCKITMLWVDKPFNPD
                     SVYIDGENFREGIHKRFAELIEEYTPVVGKDNIQYKIRTGKVYEEIAAYAKLNKCRFV
                     VIGTHGISGFEELWIGSNANRVVSIAPCPAFTLRQDFLVPDAIRKIIVPIDRTAETEL
                     KIPFAIDIAKAFNAEIVLMKLYATNVPLLKKKVDDIANDICDKLAHEKIKVSTVEKII
                     VNITSDVLSVIEKKDADLLLIMTEQQNRSANIMLGEMAQQLVNNSPIPVLSIHNDDKP
                     FN"
     gene            8457..9869
                     /locus_tag="PKY63_05705"
     CDS             8457..9869
                     /locus_tag="PKY63_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPF00141.1"
                     /translation="MYPDSLHRIAEEAFLIPVSNVTMLPASGSSRRYFRITFNNGKTI
                     LAAENAEIRENNAFIGFAKHFASHGLPVPEILHEHSNKQLYFQTDLGNETLYDFLSAN
                     ENNAEKKHEYYKLAADYLLKFQFCPPPDYSLSFPRDRFDRTSMMWDLNYFKYYFLKLA
                     NVPFDEQKLEHDFSAFCNTLDAVPASFFMYRDFQSRNIIIENNNLAFVDFQGGRRGPL
                     QYDLASLLFDAKANLGNDFRKEIFDHYLHKLSTSHPEESQQFEKYYLHFALLRIMQAF
                     GAYGYRGYYEKKSHFLQSVPLAAANIKYLLNQIGFGGDFPELLKIWKYIAENFDNNDL
                     PAPDKGVLYIDVSSFSYRKGYPSGSPDHGGGFVFDCRALPNPGREDRFKAHTGKEKIV
                     ADWLKNKEEVAFFLNSCSALVNLSVDNYIKRNFNHLSIAFGCTGGQHRSVYCAEQMAE
                     LLQKTYGEKIQLKLHHREFS"
     gene            9919..10509
                     /locus_tag="PKY63_05710"
     CDS             9919..10509
                     /locus_tag="PKY63_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF00142.1"
                     /translation="MNIALLKKGDHRAFQELIDSTHSDVFRLALKFTSNRDDAKDIAQ
                     EVYLEAFKNISDFREDANIRTWLYRITVNRSLNLIKRNKNKFHSLSIENADESIIQIP
                     TPGDHEADQAIENKELKLVLNVALSKLPDNQRTAFLLFNHDGMKYNEIAEVMNLSLSA
                     VESLIFRSKANLRNILNDYYKKNYASAQVFKNNNVK"
     gene            10506..10958
                     /locus_tag="PKY63_05715"
     CDS             10506..10958
                     /locus_tag="PKY63_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00143.1"
                     /translation="MNNESKMNCNEFCHIIDSGTISGKQMTFVAEHMAGCAQCKKYYQ
                     LMYYSIEHANELLPDETPHAGMAAEIGVFVFAAGQVSKTIPLWAKITSAAAAIAIGLI
                     IGSAVYDSGNIDTTNPETAFITDNNNPVYMAETTEILYNSFLAENEGE"
     gene            10961..11470
                     /locus_tag="PKY63_05720"
     CDS             10961..11470
                     /locus_tag="PKY63_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00144.1"
                     /translation="MKNKNTIIWILSIISIVSTTALITFWIIRPSAPDIKAFNRNLNR
                     QHNTNMHCDQNLMVRLNLDQEQKNEFNKERSLHHTTIKPIFDSLRILRTKLFNELDKE
                     NPDSAIINSCISAISDQEKIIQQESVHHILSMKKFLNPTQFDTLISIHSQAMMPVRKG
                     THNNRQHYK"
     gene            11496..12062
                     /locus_tag="PKY63_05725"
     CDS             11496..12062
                     /locus_tag="PKY63_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPF00145.1"
                     /translation="MKKLFLTTLIALFAVTFSTAQNQQGQPKAGQGQGQGQMMHKSGD
                     CNHIPNLSADQLKKMEPLRLDFQKQKMEIYNQIKAKKAQLIIVSTGDKINKAEAFKLI
                     DEIAVLRTSLEKARFEHQMAVRALLTPEQQVYYDMHHAKNGMGNGRRGHGNGYGQGQM
                     NGNGCGQKSGNGNGQGLHHGPGTCIHGN"
     gene            complement(12114..12590)
                     /gene="ispF"
                     /locus_tag="PKY63_05730"
     CDS             complement(12114..12590)
                     /gene="ispF"
                     /locus_tag="PKY63_05730"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPF00146.1"
                     /translation="MDIRQGIGYDLHRTITGKPLVIGGVNIPSDFGLDGHSDADVLIH
                     ALCDALLGALALGDIGAHFPDTDPNYKGIDSMILLKKTMDLISAQGYEILNIDAVVVA
                     ERPKLMPYKLQMAENIAAALNTDRSHVSVKAKTNEGVDAIGRGEAIAAQVVVLLGK"
     gene            complement(12600..13805)
                     /gene="porV"
                     /locus_tag="PKY63_05735"
     CDS             complement(12600..13805)
                     /gene="porV"
                     /locus_tag="PKY63_05735"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HPF00147.1"
                     /translation="MKLIVSIISVVVLVGLVSKGWSQSSQDLTEQAYGINTITTAVPF
                     LLIAPDARGGAMGDAGAGTSPDVYSMHYNPAKYAFIDKNVGFSVSYSPWLSQLVNDIS
                     LGYLTGYKKIDDDQVLAGSLRYFSLGNIVFTDNVGTEIGQFSPNEFAVDVAYSRKFSD
                     YIGGAIALRYIYSNLTGGIYVNGAQSKPGQSIATDIAVYYEKEIDMGKTPAVFSFGAD
                     ISNIGAKITYTENQNRSFIPINMRLGPSLTMDLDDYNQLMFTFDFNKLLVPTPPIYAE
                     DSAGQPIKDDNGDYVIADGKDPKRSIVSGMFGSFSDAPGGFKEEVQELSFSLGTEYWY
                     DQQFALRAGFFYEHPNKGNRKYITLGAGLRYNVFGLDFSYLIPLEQRNPLENTLRFTL
                     VFNFDKVKS"
     gene            complement(13848..17663)
                     /gene="porU"
                     /locus_tag="PKY63_05740"
     CDS             complement(13848..17663)
                     /gene="porU"
                     /locus_tag="PKY63_05740"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPF00148.1"
                     /translation="MRGLFLSLLLIISFLTRAQIGISPNLNQSVKRVFPFENTVFTDF
                     GKVPCFVDNISINNIESVFIPVLSDIRVEKINAADLPADIVSEIPSDFSMNVSTTEIK
                     KMYFARVFTGCLRKNSQTGEIERLVSYTISGQEVLKDKDNQLLKRTYAGSSVLENGSW
                     YKINTSQGGIHKISYENLENWGIDVSEIDPVNIRLFSNGPRMLPELNSTPRYDDLTEN
                     AIYVHDGGDGSFDPGDYFLFYTEGILYWTAQPDKSFYHNYNLFSDKNICFLTFDNGPG
                     LRISVAVQETLPATHQVTAFLDFMAYEQNLYNLIKSGKNWYGDNYESSSLSKSYPFSF
                     ANICNDSLVKVKAALVARSSSPSYFTVFSGSHGDTVDISSTSGYTSDYAKTGYASFSF
                     STSTSSVPVSIVYHQNGNAEAMGWLDYIEVSAWRNLVFGGNQMAFRNPNVSGSGHIAE
                     YTISNADNSLQVWDVTNPLNPLFIQGTLSSGNYTYRINNDSLRQFIAFNGVLYHTPAF
                     IGVVDNQNLHAFPQTDYLIVSYPAFRSQAAAIGQLHEQLDGYSYVVVTPEEIYNEFSG
                     GKQDPTAIRDFVKMFYDRASSADDMPKFLLLLGDASYDYKNRITGNNNLVPTFESASS
                     LSPTTSYATDDFFGLLGDTEGGDCNGSLDIGVGRIPVNSTEEAENVLRKLEKYTSPVK
                     IETSGDGCSEGTCNVSNMADWRNQICFVCDDGDGNMHFNQADNMAERIDTVNDFLNVD
                     KIYLDAYKQVSTTGGERCPEANDAIRQRVEKGALIVNYTGHGGEVGWTLERILDVPTI
                     QNWTNGCNMPVFITATCEFSRFDDPGRVSAGEYVFLNPSGGGIALLTTTRLAYASYNE
                     ALNNSFYDIAFDNSSGRFPTLGELIAHCKTDNGSVSYLRNFMLMGDPALTMTIPENQV
                     ITTEINGVSVSGFNDTISALKHVTVKGYIADPFGVKMSGFNGTLYPTVYDKAIELKTL
                     ANNPAENYVAPFMLQKSIIYKGKASIIDGEFSFSFIVPKDIQYTFGKGRISYYAENGT
                     SDASGWFEDFIIGGSSDTILDDNAGPVISLYLNSELFISGGTTNENPFILANLSDESG
                     INTVGTGIGHDITATLDGNSAESVVLNDYYEAEKDSYTNGSVRYPFKSLSEGPHTLTL
                     KAWDILNNSSEASIEFIVSSSAGLALTHVLNYPNPFTTRTEFWFEHNQPCCGLDVQIQ
                     IFTITGKLVKTITRNVNTNGFRADPIEWDGNDDFGSPLARGVYIYRLRVKNADGQYAE
                     KSEKLVILK"
     gene            17896..18894
                     /locus_tag="PKY63_05745"
     CDS             17896..18894
                     /locus_tag="PKY63_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPF00149.1"
                     /translation="MTGRITRIILATAIIAAMSVWDAKAQDPHFSQYYANPLYLNPAM
                     AGAPICPRLILNYRNQWPGISGQYVTYNASYDQHVDALSGGIGVLVNVDRAGEGTLST
                     TMASGIYSYKLDLNRKLSLKAGIQATFLQKNLDWTKLTFPDQIDPRHGFVFNTNEVYP
                     EDGLTKTVADFATGLVLYSERFFAGVSAHHLTTPNEGFISYSELPLKWTGHAGAIISI
                     EGDNSRKRTVEETSISPNIMFQKQGQFEQFNYGCYLNKYPFVVGTWFRQSLDNPDAVI
                     VLLGFVQPTFKFGYSYDLTVSKLTNASGGAHEFSLTIQFECSPRQRRVKPITCPSF"
     gene            18954..20429
                     /locus_tag="PKY63_05750"
     CDS             18954..20429
                     /locus_tag="PKY63_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPF00150.1"
                     /translation="MLNKNRIFKGFLVLGLISLSLSSCYKERSRTTAWYYNDPKWGGF
                     EVVPYAEQETGPGLILIEGGTFVMGRTEQEVPYKWDNIPRRVTVSSFYMDMAEVSNVD
                     YREYLYWLGRVFGVDYPEVVKQALPDTLVWRSRLGYNEPYVEYYLRHPAYAFYPVVGV
                     SWTQANRYCEWRTDRVNEYIMIREGILKIDPNQQNEENFNTDAYLAGQFEGVVKNDLY
                     DLNPNGSGTRKVRMEDGIMLPKYRLPTEAEWEFAAIGLIGNTLFERVIERRIYPWNGH
                     LLRTDYYKNMGEFVANFKRGRGDNMGVAGNLNDAADITAPVYSYWPNDYGLYNMAGNV
                     AEWVLDVYRPLSYEDFDDFRSFRGNEFQTKVTDEEGLIAEKDSLGRINWRPVTQEEAA
                     NRRNYRYSDNRNYLDGDYSSSVYYGDDMMKDGEFENKLMYEYSKSSMINNTARVYKGG
                     SWKDRAYWLSPGTRRFLDQELSTDYIGFRCAMIRVGSPIGF"
     gene            20483..21769
                     /gene="murF"
                     /locus_tag="PKY63_05755"
     CDS             20483..21769
                     /gene="murF"
                     /locus_tag="PKY63_05755"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPF00151.1"
                     /translation="MNIEQLYQKIKDSAGVSTDTRSLKPEMVFFAIQGEKFDANNFIQ
                     QALDAGAALVVTQNPAFEKHPQCYFTNNTLKTLQEIALWKRKQMKAIVIGITGTNGKT
                     TTKELMREALSAAGTVEATQGNLNNHIGVPLTLLSMNNDTDFAIVEMGANHPGEIEFL
                     CNIACPDYGVITNIGRAHLEGFGSFEGVIKTKTELYSFIAKNNGSVFVNEDDPLLVKL
                     SEGCKKILYGTKTLDASIVNDSNPFLRCHWKVNEEGFLTQTKLSGSYNLPNLLAAISI
                     GLHFGANAKEINKKLSEYTPSNMRSQWIETEHNQLILDAYNANPSSMSAALKNFADLQ
                     TEKKLPILGDMLELGSYSDNEHHEVLNLIKELSFKSAVLIGPCFSKFRNEFPAFLFFS
                     NTAEAKTALTKNTISGFTILLKGSRGIGVENLKEIL"
     gene            21769..22842
                     /locus_tag="PKY63_05760"
     CDS             21769..22842
                     /locus_tag="PKY63_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPF00152.1"
                     /translation="MKNYDIHEWNAGVGMMSGTSLDGVDIAICAFRFNNGVWEYRIDD
                     AVTFPYVDEVKALILEMPGMSGPELMIADRKLGKYHGQLLSGYLMAMGETPLFVASHG
                     HTIFHEPSEGMTYQAGHGAAIAAETGLTVVCDFRSSDVALGGQGAPLVPIGDELLFGE
                     FDALVNLGGFANISYRSDNQRIAYDICPVNIVFNHFAHLSGHEFDRDGQLASAGKPYS
                     ELLPMLDKIPYYSAHPPKSLGREWIEKEFMPVIEKSGIVNTDDILCTLAHHAAGQIVR
                     ACGANKSILFTGGGAKNHFLTGIIRKKLQDRMIIPDETTIDFKEALVFAFMGWLRLNE
                     ISNTLPSATGASKAISAGAVFIP"
     gene            22974..23699
                     /locus_tag="PKY63_05765"
     CDS             22974..23699
                     /locus_tag="PKY63_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPF00153.1"
                     /translation="MFLNVLLQIVQGGDSLATATANTVQKTVETANQQYDTLSFWDLS
                     LKGGPVMIPIALLLIIAVYIFFDRYIATSKAAKEDSGFMANIKHFITEGKIDQAKAMC
                     LSNPTPMARMIEKGISRIGRPLNDITTAIENEGKLQVARLEKSIAFLSTIAGIGPMIG
                     FLGTVTGMITAFYDLSKSGNNLDIALLSGGIYEAMVTTVAGLIVGILASLAYNVIVAR
                     VEKIVYILEARSTEFMDILNEPA"
     gene            23711..24136
                     /locus_tag="PKY63_05770"
     CDS             23711..24136
                     /locus_tag="PKY63_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPF00154.1"
                     /translation="MNIKTRNKRTTDFNSASMSDLVFLLLIFFMLTSTLVSPNAIKLL
                     LPSSTSERVPVEKKAVNVYIDNQFRYSIDQKILDAAGLREGIKSRLDGQTEASVVLRS
                     DATVPIQFAVNVIDIVNSINKEKGTKHKVILATRPEENI"
     gene            24141..24866
                     /locus_tag="PKY63_05775"
     CDS             24141..24866
                     /locus_tag="PKY63_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPF00155.1"
                     /translation="MKSKRKITSALLTLAFCGGVVVILFLFAFRTPLPLPEEEGVVIE
                     TNAGGGGGGSDEFFDQEFYEEMSADNIGETNEDYITQDVENVNYTSGQQSTNTTDKPK
                     VDNRLTNFSWGQGNGTGTGTGSGSGSGTGTGSGTGVGSGDGPGTGPGSGPGYSLKGRG
                     AKSIPTPKYTEDDQGKVVVTIWVDRDGKVTRAEPGAIGTTVSNPSLWTAAKNAALQAK
                     FSTNESAPEIQKGTITYTFIKLN"
     gene            complement(24936..26510)
                     /gene="nadB"
                     /locus_tag="PKY63_05780"
     CDS             complement(24936..26510)
                     /gene="nadB"
                     /locus_tag="PKY63_05780"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPF00156.1"
                     /translation="MKQYCDFLIIGSGIAGLTYALKVAPYGKVVILTKSVLDETATHY
                     AQGGIAGVMYGPDNYEKHINDTIVAGAGLNNEMIVRITIEESTERIVELINFGTHFDK
                     NTDGEYDLAREGGHSEKRIFHYKDRTGAEIQRALTKQIREHPNISVLENYFTIEIITE
                     HHLGKNVTKKSENITCFGAYVLNPETGKVDTFISKTTLIATGGSGNVYGVTTNPEVAT
                     GDGVAMVYRAKGVVENMEFVQFHPTAFYYPTEKPAFLITEALRGAGAILKTYDGNEFM
                     QKYDNRLSLAPRDIVARAIDSEMKISGADHLYLDARHIGKKKLLEHFPTIFAHCLSKG
                     IDISRDMIPVVPAAHYQCGGIKVDECGRNNIKHLYATGEVSCTGLHGANRLASNSLLE
                     AAVFSHRAALDSIEAVKNIDINNDIPVWDAEGTVLNEEMVLITQTRKELNSIMGSYVG
                     IVRSNLRLKRALDRLKILYEETEELYKKSVVTKEICELRNMISVGYLIIKMALERKES
                     RGLHYSLDYPPKESGK"
     gene            26571..27500
                     /locus_tag="PKY63_05785"
     CDS             26571..27500
                     /locus_tag="PKY63_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF00157.1"
                     /translation="MKSKIWIYIGLMFAMAVWAYSYVLTKIVYSYGVPPTMLVFMRLL
                     LATITLLAFTKITGKLQKIQKPDKISFLALAFFEPFLYFIGESNGLMLVSPTVGSVII
                     ATIPVFIPFVLYAFYKEKVDKTNFFGIIISFIGVLLVIFEDDYSLAASPVGLAFMFFA
                     VFSAFGYTIVIKKLTQRYNSFTIVTYQTVIGAVLYIPLFVFSDFSVIANVDFTWQMIA
                     ATITLAIFATAIAYILFSTGIREIGAMQSTIFANFIPVLTALLALLSGMEELPMQKLA
                     GVLIVILGVFLSQSKKPFLGLPGMVWNYFVKKK"
     gene            complement(27791..28516)
                     /locus_tag="PKY63_05790"
     CDS             complement(27791..28516)
                     /locus_tag="PKY63_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPF00158.1"
                     /translation="MKISLVSYLNSFPYHFGLLNADDGLYSQLSIVHPAQCAKNFAEG
                     SSDVALVPVGALNSIENFQIIKPYCISADGPVRSVLLLSNRPLQDIKHISPDSHSRSS
                     NLLLKILCHNHWNIHPAFVKHDDASADARIAIGDKAFELQKDYKYSFDLSEAWKEMTG
                     LPFVFAVWIARADMKDEMLKPLKTALEAGIEDPAAAVRHFGSADLSFDDAVSYLTDNI
                     NYRMNAAAEEGLKLFLSMAAKVL"
     gene            28627..29655
                     /locus_tag="PKY63_05795"
     CDS             28627..29655
                     /locus_tag="PKY63_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPF00159.1"
                     /translation="MKTTITINRLAEWAQKLRAGTKSFASGNQPEAFYEAMTHNKWFT
                     KENIIVSINALAEMISDENTVNEWLAKYPEPSSDPKKILLILAGNIPAVGFHDVLCTL
                     LSGHSAEIKLSSDDKFLIPWMLRNAEKYFPEWTDRIHYHEGLIQNFDGVIATGSNQSA
                     KTFKSYFSAKPHIIRHSRSSIAVINNNDSDEDLKKLGDDVFMYFGLGCRNVSLIFLPQ
                     GFDKQRLFDAFLSWKDLEYHNAFANNYVYYKGYFSLSKEEPLDGSFYLLRENLSTAAP
                     VAVLHYTEYTHINDVKKYIAENEEHIQCIVSTQNEIENAVKPGQAQSPRIDEYADGVD
                     TMAFLAAL"
     gene            29889..30296
                     /locus_tag="PKY63_05800"
     CDS             29889..30296
                     /locus_tag="PKY63_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPF00160.1"
                     /translation="MKLSRKCEYACLAMIELSGRNPDEYTKIESISNTWNIPRKFLEQ
                     ILITLKNAGYLHSRKGADGGYKLAKESSKISLAEIIRLIDGALAPVESVSTYFYGSSP
                     TEKHPGFINIMKDIRDYVAEKLEKTTFADLVRK"
BASE COUNT         9441 a         6755 c         6006 g         8126 t
ORIGIN      
        1 actgaacgtg aaaaatggga aaaactcact gccaaagaaa aagctgctca gttgtattcg
       61 ttcgacaaac caacaaaaga aattaccgtt acgcaggaaa tgcgtcagaa agaatttgac
      121 aatttcaaaa ccacagatga tcacggactg ctcattacag gacttgccaa agatcagaat
      181 ggcactattt atttcaaagt aaaaaacagt tgggatgctg ttggcaaata tgatgggtat
      241 ttctatgttt caaaagcatt tgtaatgctc aaaactaccg acatgctggt gcaccgcaaa
      301 ggaattccgc ctgcaattct gaaaaaactg aaactgtaat ttttattgaa gaattcacag
      361 ccggttagtt aaactgaccg gcttttttta tagctgatat tcttactcag atttcaaata
      421 catgacaatc gtcaataaaa aacattcaat ctttcaaagc attttatctt ctcttcagtt
      481 gttttataag tatttttgcg catagataaa acctgccatg aaaaacatac tcattctcgg
      541 agccggactt tcggcatcaa cactcatcag atatttactg caaaaaggtg agcaaaatca
      601 atggactgtt acagttgccg acatggatat tgaaacagcc aaaaagaaaa ccgatggtta
      661 taaaaacgga aaagctgttt cgttcaacat aaaagacgaa aaaaatctcg cagaactcat
      721 aatgcaaaac gaccttatcg tcagcatgat gcccgctctt ttccatccgg tagtagctca
      781 gcagtgtctc aacttttcca aacatctctt cactgcttca tatgtatcgc ccgagatgaa
      841 atcattcaac gaagaagcaa cggcaaaaaa cctgattttc ttcaacgaat gcggtgttga
      901 tcctggcatc gatcacatgt cggccatgaa agtgattgat gaaatccgtg ccaaaggcgg
      961 acgtatcatt gggtttgaat ccaactgcgg cggtctggta gcacccgaat ttgacaacaa
     1021 tccatggaat tacaaattta catggaacgc ccgtaacgtc atccttgcag gacaggcagg
     1081 tgcccgtttt ctgcataacg gcaaatacaa gtatatccct tacacacggc tctacgagcg
     1141 catcgacaaa acttctgtgc tgaattttgg caactttgaa atctatgcaa accgcgactc
     1201 actgagctat aaagaagttt acggactcga aaatgtagaa accattgttc gcggaaccat
     1261 gcgccgaccg ggctattgcg ctgcatggaa ctgctttgtg caactcggcg tatgcgatga
     1321 tacctacgaa ctcgaacatc ccgaaaaact aactaatcgt agttttattg aatcttttct
     1381 gccggaaaat ccgggtctga gcgtcgagga taatttctgt caatatctga aacttgaccg
     1441 caacggagaa attttcaaga aaatggaatg gctgggtctg ttcaccaaaa cccctgtggg
     1501 aaccggattt aaaacacctg cacaaattct tcaaagcatc attgaaccaa agtggcagct
     1561 cgacaaaaac gatctcgatc ttcttgttat gcaacacact tttgattacg aactgaacgg
     1621 taaaaaatat cgccgcatgt catcgatggg aatcattggc accgacacag ttcacaccgc
     1681 tatggcaatt accgtcggaa tgccgctggc tctggccatt gaacttttcc ttacgggaaa
     1741 aatccacggc aaaggcgttc aaattccggt taaacccgaa tactacaatc ctattctcga
     1801 aggtctgaag ccattcggag ttgagttctt cgaagaagaa acagagattt aaaaatgaga
     1861 atttttgtcg caatggttat cgcgatgaag atttttgatt tcattgagat aaagtcggtt
     1921 ttgaaatatc aataaccggc ttttcttgtt atagatgttg tttataaacg atatatacaa
     1981 ctgacagctg aaactgtcag ctatgatgaa tatcctcgac cttaatctta atctgggcgc
     2041 tcgtctctcc tgtgctgatg aaggaacttc cgtcggcatg ccgcctaccc cctgaccttg
     2101 tgtttactcc gcaaacttcc gcagataggc agtcagatcc gaggcaatat ccagcttcag
     2161 ttttttcgac aggcgattct ttttgatttt gatcgatgct tcggaaatac ccatcacatg
     2221 agcaatttcc ttgttgctta aattaatcag aatgtatgca caaagtttct gctctccctg
     2281 agtaatatca ggatgagttt cagacaaaac tttgaaaaat cccggataca cctgatcgaa
     2341 atgataaaaa taattattcc agacagcttc atcgccggca ttcatgcgga tgatgctctg
     2401 aatttttttc agcgaagcat catttccttt atctgtattc gcagaaacaa actgatcaat
     2461 atttttctca atttcctgca atgtttcgtt tttggttaca atatgcatgg tcattgatgt
     2521 caattgccgc tctttcatct gcaggtcatt ggtaagtttt tccttttcca actgagcaat
     2581 aatggccttg ttttccaatt cttcggacag acgacgtttt tcgctttcag ccagggcatg
     2641 cctctgatca agcatacggt ttcggttacg aatgttgata aaaagaagga caaacaaaac
     2701 aagtgcaacc gcgataattg caatgatgag ccactgaaca tgattttgca atttcatttg
     2761 cttgtcaagc aaagattttt ccttctgcca tttgctttgc tgcatcgata aaatgaaatc
     2821 ggcgaaatca ccttctgtag tctgtgactc aattttctca gttatatcct tgtatttttt
     2881 cagattttca tatgctttgg caatctcgcc tgttgactca taatactgag aatacaattt
     2941 ataaatctcc ggaagctgag cagatgcatc gctttcaatg gcattgaaca atgctgaatc
     3001 aagatattca gcagcacttt tactgtcgcc gccattaagc agtgttcgtg caagattact
     3061 gtaagtcagc gccagctgcg actttgcgat gctgtcatga aaagcaagtg atgccttgta
     3121 ataaaaaacg gcgctgtcac ggtttcctgt attttcgtaa atcactgcaa gattattcat
     3181 agccgttgca atgccaacag gataatcatg ctgcctgctg attaaaatcg actcacgatg
     3241 cagagccatt gcagtatcat acatacgaag cttctcatac aatgatccga tattgttttt
     3301 cagatcaaca atgtttctga atttctttgc tctgatctga attttcagtg actgattgaa
     3361 aaagaaaata ctgtaatgat atttgctgat ggtgtatgaa acaactccaa ggttattgta
     3421 aacaagcgat attccaacag tatcatcaac gctctgataa tattcaagcg aacggataaa
     3481 actctgcagc gccatgtcat tcagcccgcg cgtgaacaat acaattccct gcaggttgta
     3541 acactcggcc agacctttct cataattcag atgagaacat tcgtcaatca gcatttctgc
     3601 ctcacgcaag gcctgcaacg gctcggaatt ggttttaccc cttgctgaca gaatacgctt
     3661 gtcaatctct tcaaactgcg caatggcaga aaaagagaca aggaaaagga atatatgcag
     3721 cagaattttt ctcacgattc aaagttatgc attttttaca tccagattgt cgcgcagttc
     3781 attgccaagt atcataaagg ccatgactgc aaggactatg gccaatcccg gcaataaagc
     3841 aagatacgcg ctgtcgagca ccagatgact gtaatactcc ttcaccatac caccccaggt
     3901 ggcaaccggc ggctgcaccc caattccaag aaaactcaac cctgcttcgg tcatgatggc
     3961 cgatgaaaac gaagatgctg ccaacacaat aacaggcccg cgtatgccag gtaatatatg
     4021 ccggaaaata atttttctgt ttttaattcc cagcgcttgt gcggcctcga tataatcccg
     4081 ctgcttgatg gacatcacct gaccacgcac cacacgagct atatcgaccc agaaagtaag
     4141 tcctatggca acaaaaatct gccagaaacc ttttcccaat gcaaaagtaa tggcaataac
     4201 cagcagtatt gttggcaatg accacaccac actgaacaac cataccagga aacgatcaat
     4261 aataccacca taataaccgg caatggcacc cagcataata ccaatcaaaa gcgcaataaa
     4321 aactgccacc agtccaaccg taatactgac acgcgcaccc aacacaatac ggctgaaata
     4381 atcgcgtcca aaacgatcgg tacccaacag aaatttcttt tttacaatca tctttgatgt
     4441 aatgaacttt tcaagttcta ttccggaaga aacccgtatc actccattcc ttttttccca
     4501 tgctgtctgc aactttcggc aatcatatac ttcaatctgc ccactattgg gtgtaaggcc
     4561 ggtatatgtt tcaatatata aactgtcttt tctgaaataa tagctgaaaa tagctttgct
     4621 actaaccgac aagggttcgc catacaacag cttttcaaaa aatgaaacct tttctgatga
     4681 agcaattgga atttcgagaa acctgcactc aaaacccggc ttctgaagtg caatttccac
     4741 ctgctgacta ttggcgtcgg aggttgcgtc gggaataatg aagtatccaa aaacagctac
     4801 cagagccatc aggatgatat atcccagaga ggtgtaaccc atgacggttt tgatcagttt
     4861 tcgtttcata tcaatcaaag acaatgacaa agatattaat ttcgttgata cattaatggt
     4921 caatcaaatt catgattgca tcgcaactta atccaattgg catgatattt tttactatct
     4981 ttaaccccgt aaaccagcat tatgaaaacc ctgatgacca ttagtctgat tgccctgttt
     5041 gtgaacctgt tcgctcagga tattcccgtg gcaaccgcag aacaattcaa acgtttcaag
     5101 gaaagcacca cttatctggt gaaatacgac gatccgttca gttcattcaa tgaatatatc
     5161 accgaagata tgtcgaaagt gtggaaaata acacctttca aaatcatttc accggatgaa
     5221 tttgaaacac tcagcaaaga caaaaaaaat tcatttattt ttctgtccga aatatttaaa
     5281 gaagaaggag aaaaaacagc aaaatggaat ttattgaaca tcacactggg atcaaatgca
     5341 ggaagcgcaa acatgaattc aatgcctgac cttggaagcg ctccgctaaa ctatgtttcg
     5401 gacaatgaag acaacgaaga agaatatctc tacaaactgg ccggcgtgct tcgttttttt
     5461 caatactaca ttcaatacaa catggcaaac cccggatccg atattaaatc cgttgtaaaa
     5521 aacaatcaat caaaattaac agggaaagaa ctctggctgc tacagagcga tatgtctgat
     5581 gaagtaaact cgctggaaaa aatcagtaaa tatttctccg gcacagtaaa atttgtcacc
     5641 cccgaagaaa tccgctcagc cattcatgcc ggaaatgaaa aggttgtttt tctgcatcag
     5701 gttacaccag gcaacagcgt cggcaaatgg agtctgaaat tcctgctctg cgcctcagac
     5761 ggctgcccgg tttattatga cgtaacaact gttggtgctg gcaaaaatgg cagattttca
     5821 gcatccgatt tcaaagcaat taactaatct ctcttttttt atgagcgacg acaaaaaaat
     5881 cattttttcg atggtgggtg taaacaaaat ttatccacct cagaagcagg ttctcaaaaa
     5941 tatttatctt tcattctact acggcgccaa aattggtgtt ttgggtctga acggcagtgg
     6001 aaaatcaaca ctgcttaaaa ttattgccgg aaaagaaaat cccaccgaag gcgacgtggt
     6061 tttctcaccc ggatactcca tcggaatgct ggagcaggag ccacatctcg acaacaacaa
     6121 aaccgtgaaa caaattgtcg aggaagcggt acaacctatc gttgacctgc taaaagaata
     6181 cgaagaagtg aacaacaaat tctcagagcc catgagcgac gatgagatga acacactcat
     6241 tattcgccag ggcgaactca ccgaactgat agagcaacac gatgcatggg agctggattc
     6301 acgcctcgaa agagccatgg atgcattgaa ttgtccacca tcggatcaga ttgtaaaaaa
     6361 cctcagcgga ggcgaacgtc ggcgtgtggc gctggtgcgg ttgctgctgc aaaaacccga
     6421 catcctgttg cttgatgagc ccaccaacca cttagatgct gaatcggtgc aatggctcga
     6481 aaaacatttg caggaatacc agggaactgt tattgctgtt acccacgacc gctattttct
     6541 cgataacgta gccggttgga ttcttgaact ggaccgagga gaaggcattc catggaaagg
     6601 caattacagc agctggcttg aacaaaaatc ggcacgtctg gcacaggaag aaaagaccga
     6661 atcgaaacgc cgcaaaacac tcgagcgcga acttgaatgg attcgcatgg ctccaaaagc
     6721 acgacacgcc aaagggaaag cacgtttgag tgcatacgaa aaattactga gccaggatgt
     6781 gaaagaaaaa gaacaaagac tcgaaatctt catccccgac ggccccagat tgggcaatca
     6841 ggttatagaa gcaaaaaata tcagtaaagc gttcgacaat aaactgttat ttgaaaatct
     6901 gagttttacc attccaccca atgccattgt tggtattatc ggtcccaacg gtgccggaaa
     6961 aacaacattg ttcagactca tgatgggtat tgacaaaccc gatagcggag attttaccgt
     7021 aggcgatacc gtgaaactcg gatacgttga tcaggcacat tcctcaatag attctgaaaa
     7081 aacggtttac caaaccatca gcgacggcat ggaaaatatc cgaatgggaa gtcgcgaaat
     7141 caatgcccgg gcctatgtaa gccgtttcaa ttttactgga cctgatcagg aaaaaaaatg
     7201 cggtgtgtta tcgggcggag agcgcaacag attgcatctt gcgctgactt taaaagaaga
     7261 ggccaatgta cttttgctcg atgaaccatc caatgacatt gacataaata cactcagagc
     7321 attggaagaa gggattgaat cttttgccgg ttgcgccatg gtcatcagcc acgaccgttg
     7381 gtttctcgat cgcatttgca cacatattct ggcattcgaa ggcgatgcat ctgtggtctt
     7441 tttcgaaggc agcttttccg aatacgaaga aaaccgcaaa aaacgtcagg gcgacacacc
     7501 tgtcagattc aaatataaaa agctatccgt ttaacaatat ttacgggtta atagttgctt
     7561 tttataacac aaattgatta acttgcataa aaattcaaca ccatgaatga aatagttgtc
     7621 ggaattgact tttcaaaagg tgcactaaat gcactcaaat atgcatcact ccttgccaag
     7681 ggttttaact gcaaaataac catgctgtgg gtcgataaac catttaatcc cgacagcgtg
     7741 tatatcgatg gtgaaaactt tcgtgaaggc atccataaac gttttgcaga gctcatcgaa
     7801 gaatacacac ccgttgttgg aaaagataat attcaatata aaatacgcac tggcaaggtc
     7861 tatgaagaaa tcgcagcgta tgccaaactg aacaaatgcc gctttgttgt tatcggaacg
     7921 catggtatca gcggcttcga agaattatgg attggctcca atgccaacag agtcgtttcc
     7981 attgcaccct gcccggcatt tacattgcga caggattttc ttgttcccga tgcaatccgt
     8041 aaaattattg ttccgattga ccgtacagca gaaactgaac ttaaaatacc atttgccatt
     8101 gatattgcaa aggcattcaa cgccgaaatc gttctgatga aactttatgc aaccaatgtt
     8161 cctttgctga agaaaaaagt ggacgacatt gccaatgata tctgcgataa acttgcccat
     8221 gaaaaaatca aagtcagcac cgttgaaaaa atcattgtca atattacaag cgatgtgttg
     8281 tcggtaatcg aaaaaaaaga tgctgattta ttactgatta tgaccgaaca acagaaccga
     8341 tcggcaaaca tcatgctcgg cgaaatggct cagcaactcg taaacaacag cccaattccc
     8401 gttttgagta ttcataacga cgataaacca ttcaattaat tttttaagtc tttttcatgt
     8461 atcctgactc acttcaccgg attgccgaag aagctttcct gattcctgtt tccaatgtga
     8521 ccatgcttcc tgcatccgga tccagcaggc gttatttccg catcaccttc aataacggca
     8581 aaaccattct ggctgccgaa aatgctgaaa tccgcgaaaa taatgcgttc atcggttttg
     8641 caaaacactt tgcttcgcat ggactgcctg taccagaaat actgcatgag cacagcaaca
     8701 aacaactata tttccaaacc gatctgggga acgaaacact gtatgatttt ctttccgcca
     8761 acgaaaacaa tgctgaaaaa aaacatgaat actataaact ggcggccgac tatctgctga
     8821 aatttcagtt ctgccctcca cccgactatt cacttagctt cccgcgcgat cgcttcgatc
     8881 gtaccagcat gatgtgggat ctcaattatt tcaaatatta cttcctgaaa cttgccaatg
     8941 ttccttttga tgaacaaaaa ctggagcatg atttttctgc attctgcaat actcttgatg
     9001 cagtacctgc ctcttttttt atgtaccgtg attttcaatc aagaaatatt attatagaaa
     9061 acaacaatct ggcttttgtt gattttcagg gaggaagacg tgggccgctc caatacgatc
     9121 ttgcgtcatt gctctttgat gccaaagcca atctcggcaa cgatttccgc aaagaaatat
     9181 ttgatcatta tcttcacaaa ctttcaacat ctcacccgga agaaagccaa caatttgaaa
     9241 aatactacct ccactttgca ttgcttcgga tcatgcaggc atttggcgcc tatggttata
     9301 ggggatacta tgaaaaaaaa tcacatttcc tgcaatccgt gccactggca gctgccaata
     9361 tcaaatattt gctgaatcaa atcggatttg gcggcgattt tcccgaactg ttgaaaattt
     9421 ggaaatatat tgccgaaaac tttgacaaca acgatttgcc ggcaccagac aaaggagtat
     9481 tgtacatcga tgtttcaagc ttttcatacc gcaagggata cccatcgggc agccctgatc
     9541 acggcggcgg ttttgttttc gattgccgcg cactaccaaa tccaggacga gaagaccgtt
     9601 tcaaagctca tactggaaaa gaaaaaattg ttgccgattg gctgaaaaac aaagaagaag
     9661 ttgcattttt tctaaacagt tgttcagcac tggttaacct ttcggtcgat aactatatca
     9721 aacgaaattt taatcacctt tccattgctt tcggctgtac gggaggtcag caccggtcgg
     9781 tttactgtgc cgagcaaatg gctgaattgc ttcagaaaac ctacggtgaa aagattcaac
     9841 tgaaactaca ccaccgggaa ttttcataac agcgcaataa aagaactttt tcattgtcta
     9901 agtttacaaa acaaggttat gaacatcgct ttattaaaaa agggagacca tcgggctttt
     9961 caggagctca ttgattccac tcacagcgat gtattcagat tggcattgaa attcacatcc
    10021 aaccgcgacg atgccaagga tatagctcag gaggtttatc tcgaagcttt caaaaacatc
    10081 agcgatttca gagaagatgc caatatcaga acctggctgt acagaatcac cgtcaaccgg
    10141 tcgctgaacc ttatcaaacg aaataaaaac aaatttcatt cgctcagcat cgaaaatgca
    10201 gacgagagca taattcaaat tccgacacca ggagaccacg aagctgatca ggccatcgaa
    10261 aacaaagagt tgaaactcgt actgaatgtt gcactgtcga aattgccaga caatcagcga
    10321 accgcctttc ttctttttaa ccatgatggc atgaaataca acgaaattgc ggaagtaatg
    10381 aacctgtcgc tcagtgctgt ggaatctctg atatttagat ctaaagccaa tttgagaaac
    10441 attctgaatg attattacaa aaaaaattat gcttcggcgc aagtttttaa aaacaacaat
    10501 gtcaaatgaa caatgaatcc aaaatgaact gcaacgaatt ttgccatatt attgacagcg
    10561 gaactatttc cggaaaacaa atgacgtttg tagcagaaca catggctgga tgtgcacaat
    10621 gcaaaaaata ttatcaatta atgtattaca gcattgaaca cgccaatgaa ttattgccag
    10681 atgaaactcc acacgcagga atggccgccg aaataggagt atttgtgttt gctgcgggtc
    10741 aagtgtcaaa aactattccc ttatgggcaa aaattacttc agctgctgca gccattgcca
    10801 tcggattaat aatcggaagt gctgtatatg actccggaaa tatagataca acaaacccgg
    10861 aaacagcatt tattaccgac aacaacaatc cggtatatat ggccgaaaca accgaaatac
    10921 tgtacaattc atttcttgct gaaaacgagg gagaataacc atgaaaaata aaaacaccat
    10981 catctggatt ctttctataa tcagtattgt ttcaaccact gctcttatta ccttttggat
    11041 tattcggcca tcggcacccg atatcaaagc attcaaccgg aaccttaacc gccaacataa
    11101 cacaaatatg cattgcgacc agaatctgat ggtgcgcctc aatctggatc aggaacagaa
    11161 aaacgaattt aacaaagaac gctctttaca tcacacaacc ataaaaccca tatttgattc
    11221 cctgcggata ctgcgcacaa agctatttaa tgaacttgac aaagaaaatc ccgactctgc
    11281 aatcatcaat tcatgcatca gcgctatttc cgatcaggaa aaaataattc agcaggaaag
    11341 tgttcatcac attctcagta tgaaaaaatt tctcaatcca acacaattcg acacgctgat
    11401 ttcgatacac tcacaagcca tgatgccggt acgcaaaggc acacacaaca acagacaaca
    11461 ttacaagtga aaaacaacaa ttaaaaacaa ataaaatgaa aaaactcttt ttaacaacac
    11521 tcattgcgct gtttgcagta acattcagca ccgctcagaa ccagcagggc caacccaaag
    11581 caggacaagg tcagggacaa ggccagatga tgcacaaatc aggtgattgc aatcacatcc
    11641 caaatctcag tgcagatcaa ttaaagaaaa tggaacctct gcgcctcgat tttcaaaaac
    11701 aaaaaatgga aatatataat caaatcaaag caaaaaaagc tcagctgatt attgtttcca
    11761 ctggcgataa aatcaataaa gcagaagctt tcaaacttat tgatgaaatt gccgtactga
    11821 gaacctcgct cgaaaaagcc cgctttgaac atcaaatggc cgtcagagct ctgctcaccc
    11881 ccgagcaaca ggtgtattac gatatgcacc atgccaaaaa cggaatgggt aacggacgcc
    11941 gcggtcatgg aaacggctat ggtcaaggac aaatgaatgg aaatggctgt ggacaaaaat
    12001 caggaaacgg caatgggcag ggattgcatc atggacccgg aacctgtatt catggaaatt
    12061 aaacctttgg ttggtttata atttgcaaag ggccttcctt cgggagggct tttttatttt
    12121 cccaacaaca ccaccacttg ggcagcgatg gcttcaccgc ggccgattgc atcgactcct
    12181 tcgttggttt tagctttcac cgatacatga ctgcggtcgg tattcagtgc agccgcaatg
    12241 ttttcggcca tctgcaattt ataaggcatc agcttgggac gctcggcaac aacaacagca
    12301 tcgatattca aaatttcgta gccctgcgcg gaaataaggt ccatggtttt tttcagcaaa
    12361 atcatgctgt caattccctt ataattcgga tcggtgtcag ggaaatgcgc tccgatgtcg
    12421 cccaaagcca gagcgccaag caacgcatcg cacaaggcat gaatcaatac atcagcatcg
    12481 ctgtggccat caagtccgaa atcagaagga atatttacgc caccaatgac aagcggcttc
    12541 cctgtaatag tccggtgaag atcataacca attccctggc ggatgtccat tgattagttt
    12601 tagcttttaa ccttgtcgaa attgaaaacc aatgtaaaac gcagtgtgtt ttcaagtggg
    12661 ttgcgttgct ccagcggaat cagataagaa aagtcgagtc cgaaaacgtt gtatcgcaaa
    12721 ccggcaccca atgtaatata cttgcggttt cccttattgg ggtgctcata aaaaaagccg
    12781 gcccgcaaag caaactgctg atcgtaccaa tattctgtac ccaacgaaaa actcagttcc
    12841 tgaacttcct ccttaaaacc accgggcgca tccgaaaagg agccaaacat accactaaca
    12901 atggatcttt taggatcctt accgtcagca ataacataat ccccgttgtc atccttgatg
    12961 ggctgacctg ctgaatcctc agcataaata ggcggtgtag gcaccagcag cttattgaaa
    13021 tcgaaagtaa acatcagctg attataatcg tccagatcca tggtgagtga tggtccaagt
    13081 cgcatattaa tcggaatgaa ggaacggttc tgattttcgg tgtaggttat ttttgcacca
    13141 atatttgaaa tatcggcacc aaaggaaaaa acagcaggcg ttttgcccat gtcgatttct
    13201 ttctcgtaat aaactgcaat atccgtggca attgattgtc caggttttga ttgtgcaccg
    13261 tttacataaa taccgccggt aagatttgaa taaatataac gcaatgcaat ggcaccaccg
    13321 atataatcag aaaacttacg gctgtaagcc acatcgactg caaattcatt cgggctgaac
    13381 tgaccgattt ccgtaccaac attgtcagta aaaacaatat tccctaaaga aaaatacctc
    13441 aaagaaccgg ccagaacctg atcatcatca atttttttat aacctgtaag ataacccaaa
    13501 ctgatatcat tgaccagctg gctcaaccac ggactatagg aaacagagaa tccaacattt
    13561 ttgtcaataa aagcatattt ggcgggatta taatgcatgg aataaacatc aggagaagta
    13621 ccggctccgg catcgcccat ggcaccaccc ctggcatcgg gagcaatcag aagaaaaggt
    13681 actgcagttg taatagtgtt aataccatag gcctgttctg tcagatcctg acttgattgt
    13741 gaccatcctt tagagactaa accaacaaga acaaccactg aaattattga aactatgagc
    13801 ttcatatgaa atctgttatt taggtttgca aatttacaat ttaatattta cttcagaata
    13861 accagttttt ccgatttttc ggcatactgg ccgtcggcat tcttgacacg gagacgataa
    13921 atataaacac cccgtgcaag cggagatcca aagtcatcgt tgccatccca ctctatcgga
    13981 tcggcgcgaa atccattggt gttaacatta cgtgtaatcg ttttcaccag ctttccggta
    14041 atggtaaaaa tctgaatctg tacatcaaga ccacagcatg gctggttgtg ttcaaaccag
    14101 aattcagtac gcgtggtaaa aggattggga taattcaaca catgagtcaa tgcaaggccc
    14161 gccgaagatg aaacaataaa ttcaatgctt gcttcagatg aattattgag aatatcccat
    14221 gctttcaagg ttagtgtatg cggaccttcg ctaagcgatt tgaacggata acgaacggag
    14281 ccgttggtgt aactgtcttt ttcagcttca taataatcat taaggactac agactcggcc
    14341 gaatttccat cgagtgttgc ggtaatatcg tgtccgatgc cggtaccaac cgtattgatt
    14401 ccgctttcat cgctcagatt ggccaaaata aaaggattct cattggtggt tcctccgctt
    14461 ataaacagtt cggaattaag atagagactg ataaccggcc ccgcattgtc gtcaagaatg
    14521 gtatcagagc tgcctccaat aataaaatct tcaaaccatc cgctggcatc agatgtcccg
    14581 ttttcagcat aataactgat acgcccttta ccaaaagtgt actgaatatc tttggggaca
    14641 ataaatgaaa aactgaattc accatcaata atactggcct ttcctttata aatgatgctt
    14701 ttctgcaaca taaagggtgc tacataattt tcagccggat tattggccaa cgttttcaat
    14761 tcaatggctt tatcataaac agtgggatat aaagtgccat tgaacccact catttttact
    14821 ccgaaaggat ctgcaatgta ccctttaacg gtgacatgct tcaatgccga aatggtatcg
    14881 ttaaaacctg aaacactcac tccgttgatt tcagtagtaa taacctggtt ttccggaatg
    14941 gtcatggtaa gtgcgggatc gcccatgagc ataaaattgc gtagatatga cacagagccg
    15001 ttatcagttt tacaatgtgc aattaattct cccagcgtag gaaaccgccc actgctgtta
    15061 tcaaaagcta tgtcatagaa agaattattc agggcctcgt tgtaacttgc ataagcaagc
    15121 cgggttgttg tgagcaatgc aattccacca cctgaaggat tcagaaaaac atattcaccc
    15181 gctgaaaccc tccccggatc atcaaatcgg ctaaactcac aggtggctgt tataaacacc
    15241 ggcatattgc agccattggt ccagttttga atggttggaa catcaagaat gcgttccagt
    15301 gtccagccaa cttcgccgcc atggcctgta taattgacaa tcagagcgcc tttttcaacc
    15361 ctttgccgaa tggcatcatt ggcttcgggg caacgctcac cgccagtagt gctgacttgc
    15421 ttatatgcat cgagataaat tttatccaca ttcaaaaaat cattgacagt atcaattctt
    15481 tcggccatgt tgtctgcctg attaaagtgc atgttcccat cgccatcgtc gcaaacaaag
    15541 caaatctgat tccgccagtc ggccatattt gaaacattgc aggttccttc ggaacatcca
    15601 tcacccgaag tttcaatttt taccggcgaa gtatattttt caagttttcg caacacattt
    15661 tcggcttctt cggtagaatt gaccggaata cgacccaccc cgatatccag actaccatta
    15721 cagtcacctc cttccgtatc gccaagcaaa ccaaagaaat catcggtagc atatgaagtt
    15781 gtgggtgaaa gggaactggc agattcaaaa gtcggaacaa gattgttatt gccagtaatt
    15841 ctatttttat aatcatacga tgcgtcgccc agcaacaaaa gaaatttggg catatcatcg
    15901 gccgaagaag ccctatcgta aaacattttc acaaaatcgc ggatagctgt tggatcctgc
    15961 ttgccacctg aaaattcgtt gtaaatttct tcaggggtaa caacaacata actatatcca
    16021 tccagttgtt catgcaactg accgatagct gccgcctgcg agcggaacgc gggatatgat
    16081 acaatcagat aatcggtttg cggaaaggca tgcagattct gattatcaac aactccaata
    16141 aatgcagggg tatgatacag tacgccatta aatgcaataa actgtctgag actatcattg
    16201 ttgattctgt aagtataatt gcccgaggaa agtgtgccct gaataaaaag aggattcaat
    16261 ggatttgtaa catcccatac ctgaagcgaa ttatcagcat tgctaattgt atattcagct
    16321 atatgacctg aaccactgac attcggatta cggaaagcca tttgatttcc gccaaaaaca
    16381 agattacgcc acgccgaaac ttcgatataa tcgagccatc ccatcgcttc ggcattccca
    16441 ttctgatgat aaacaataga caccggaacc gacgaagttg aagtagaaaa agaaaaagac
    16501 gcatatccgg ttttggcata atcgcttgta taacctgagg ttgaagaaat atcaaccgta
    16561 tcaccatgag aaccggaaaa aacagtaaaa tagctgggac tcgacgaacg ggcaaccaat
    16621 gcggccttca cttttaccag agagtcgttg cagatattgg caaaagaaaa cgggtaactt
    16681 tttgaaagcg aagaagactc ataattatca ccataccagt ttttaccact tttaatcaga
    16741 ttgtacagat tctgttcata agccataaaa tccaaaaaag cagttacctg atgagtggcc
    16801 ggcaaagtct cctgaactgc tacagaaata cgcaaacccg gtccattatc aaatgtaagg
    16861 aagcatatat tcttatcact gaacaaatta taattgtgat aaaaagattt atccggttgc
    16921 gctgtccaat aaaggatgcc ttcggtataa aacaaaaaat aatcgcccgg atcaaaagaa
    16981 ccatcaccac catcatggac ataaatggca ttttcggtca ggtcatcgta tcggggcgta
    17041 ctgttcagct cgggcagcat gcggggccca tttgaaaata agcggatatt gaccggatcg
    17101 atttccgaaa catcaatgcc ccagttttca agattttcat aactaatttt atgaatacca
    17161 ccctgcgatg tgtttatttt ataccaggag ccgttttcca aaaccgaact tccggcataa
    17221 gttctcttaa gcaactgatt atctttatct ttcaaaactt cctgcccact gattgtataa
    17281 ctgaccagac gttcaatttc gcctgtctga ctgtttttgc gaaggcatcc agtaaaaaca
    17341 cgggcgaaat acattttttt tatttcagtg gtcgaaacat tcattgaaaa atcagatgga
    17401 atctctgaaa caatgtctgc gggtaaatca gctgcattta ttttctcaac ccgaatatca
    17461 gacaaaacag gaataaagac gctttcaatg ttattaattg atatattatc gacaaaacaa
    17521 ggcactttgc cgaaatcagt aaacacggta ttttcgaagg gaaaaacgcg tttgactgat
    17581 tgattcagat tgggagaaat cccgatttgc gcccgagtca aaaagctgat aatcaacaac
    17641 aaagacaaaa aaaggcctct cataaaacaa aaagaatcag ttacacaccg gcaaaataac
    17701 aacaattggc gggatttatt gtttgatggt gaaaaatttg tatttttgaa aagattattt
    17761 acaaaaggta aaatcgattg aataaattct tttatctttg taacctttca aagaaaaacc
    17821 aagtataaaa ttttcggaac gaattggtgt ttttttgaaa gtatttgata tttttgtcat
    17881 taaattcttc aatccatgac aggacgtata acgcggatta tcttagccac agctatcatt
    17941 gctgcaatgt cggtatggga cgcaaaagcc caggatccac atttttcgca atactacgcc
    18001 aacccgttat atctgaatcc ggcaatggca ggtgcgccca tctgtccacg tctgattttg
    18061 aattaccgga accaatggcc tggtatttct ggacaatatg tcacctacaa tgcatcgtac
    18121 gatcagcatg tagatgcctt gtcgggtgga attggcgttc tggtcaatgt tgaccgtgca
    18181 ggtgaaggca cgctgagtac aaccatggcc agtggaattt attcatacaa attggatctg
    18241 aaccggaaat tatcgctcaa agcaggtatt caggccacat ttctgcaaaa gaatcttgac
    18301 tggacaaagc ttaccttccc cgatcagatt gacccacgtc acggatttgt tttcaacacc
    18361 aacgaagttt atcccgaaga tggcctcact aaaactgtag ctgattttgc cacgggactt
    18421 gttttataca gcgaaagatt ttttgctggc gtttctgctc atcacctcac cactccaaac
    18481 gaaggattca taagctattc ggagctgccg ctcaaatgga caggacacgc aggagccatc
    18541 atcagcattg aaggcgacaa cagccgcaaa cgtactgttg aagaaacaag catctctccg
    18601 aatattatgt ttcagaaaca aggacaattt gaacaattca actatggctg ttatctcaac
    18661 aaatatcctt ttgttgtcgg aacatggttc cgtcaaagtc tcgacaatcc cgatgcagtt
    18721 atcgttctgt tgggctttgt tcagccaact ttcaaattcg gctacagtta cgaccttacg
    18781 gtttcaaagc tgaccaacgc ttcaggcggc gcccatgagt tttcactcac cattcaattt
    18841 gaatgctctc caagacaacg aagggtaaaa ccaattacct gtccatcttt ctaacacaat
    18901 aacaacacgc atttttcagt tattaaataa attgaaccga aaatttcacg aacatgctca
    18961 ataaaaacag gattttcaaa gggtttctag ttctggggtt gatttccttg tcgctttctt
    19021 catgctacaa agaaagatcc cgcacaactg catggtacta caacgacccc aaatggggtg
    19081 gtttcgaagt agttccttac gctgaacaag aaacaggacc aggcctcatt cttattgaag
    19141 gcggtacatt tgtcatgggt cgaaccgaac aggaagttcc atacaaatgg gacaatatcc
    19201 ctcgcagggt cactgtatca tccttttata tggatatggc agaagtgtca aatgttgatt
    19261 accgcgaata tctctactgg ttagggcgcg tttttggcgt cgattacccc gaagtagtaa
    19321 aacaagcgtt gcccgacacg ctcgtctggc gctcgcgact gggttacaat gaaccttatg
    19381 tagaatatta tctgcgccat ccggcttatg cattttatcc tgtagtaggt gtgagctgga
    19441 cacaggcaaa ccgttattgc gaatggcgaa ccgaccgcgt gaatgaatac atcatgattc
    19501 gtgaaggtat tctgaaaatc gaccccaacc agcagaacga agaaaacttc aataccgatg
    19561 catatctggc cggtcaattc gaaggtgtgg ttaaaaacga cctgtatgat ctgaatccca
    19621 atggctcagg aacacgaaaa gtgcgcatgg aggacggcat catgctgccc aagtatcgtc
    19681 tcccaaccga agccgaatgg gaattcgctg caattggtct tattggaaac accttgtttg
    19741 aacgcgtaat tgaacgcaga atttatccgt ggaacggcca cctgcttcgt accgattatt
    19801 ataaaaatat gggtgaattc gtagccaact ttaaacgtgg acgcggcgac aacatgggtg
    19861 ttgccggcaa tctgaacgat gccgccgata tcactgcgcc cgtttattca tactggccca
    19921 atgattatgg cctttacaac atggccggca acgtggcaga atgggttctc gatgtctatc
    19981 gtcctctttc ttatgaagat tttgacgact tccgctcttt ccgtggtaat gagtttcaaa
    20041 ccaaggttac agacgaagaa ggtctgattg ctgaaaaaga cagtctcggt cgtatcaatt
    20101 ggagacctgt tacgcaggaa gaagctgcaa accgccgcaa ctaccgttat tctgacaacc
    20161 gtaactatct cgatggcgac tattcatcat ccgtttatta tggtgatgac atgatgaaag
    20221 acggtgagtt tgaaaataaa ctcatgtatg aatactcaaa atcatcgatg attaacaata
    20281 ctgcccgcgt ttataaaggt ggttcatgga aagaccgtgc atactggctc agcccgggaa
    20341 cacgccgttt cctcgatcag gaactctcaa cagactatat cggattccgt tgcgccatga
    20401 ttcgtgttgg cagcccgatt ggattctgat acattcgaaa aataattaac tttaccccca
    20461 tctaacccat gggggttttt ttatgaatat tgaacaactc tatcaaaaaa tcaaagattc
    20521 agcaggcgtt tctaccgaca cccgctcatt aaaacctgaa atggttttct ttgccattca
    20581 aggcgaaaaa ttcgatgcca ataattttat tcaacaagct ctggacgcag gagcagcact
    20641 tgttgttact cagaatccgg catttgaaaa acatccacaa tgttacttta caaacaacac
    20701 cctcaaaaca ctgcaggaaa ttgccttgtg gaagcgcaaa cagatgaaag ccattgtcat
    20761 cggaattacc ggaaccaatg gcaaaaccac taccaaagaa ctgatgcgcg aagccctttc
    20821 agctgctggc acagtcgaag caacccaagg caatcttaat aatcacattg gcgttccgct
    20881 cacattgctt tcaatgaaca atgacacgga ttttgccatc gtggaaatgg gagccaacca
    20941 tcccggtgaa atcgaattcc tgtgcaatat agcatgtccc gactacggag tcattaccaa
    21001 cattggtcgg gctcatctgg aaggatttgg cagctttgaa ggcgtaataa aaaccaagac
    21061 tgaactgtat tcttttatcg ccaaaaataa tggaagcgtt tttgtcaatg aagacgaccc
    21121 actcctggtg aagctttccg aaggttgcaa aaaaatcctg tatggaacca aaacgcttga
    21181 tgcatccata gtaaatgatt caaatccttt cctgcgctgc cattggaagg ttaatgaaga
    21241 aggatttttg actcaaacaa aactaagcgg ttcttacaat ctcccgaatt tgcttgcagc
    21301 tatttccatc ggactacact ttggcgccaa cgcaaaagaa ataaataaaa aactatcgga
    21361 atacacccct tcgaatatgc gttcgcagtg gattgaaaca gaacataatc aactgattct
    21421 cgatgcatac aatgccaacc cttccagtat gtctgcggca ttgaaaaact ttgccgatct
    21481 tcaaacagaa aaaaaactcc caatactcgg ggacatgctt gaacttggct cttacagcga
    21541 caatgaacat catgaagttt taaaccttat caaagaattg tcattcaaat cagccgttct
    21601 gattggccca tgtttcagta aattcagaaa tgaatttccg gcatttttat tcttcagcaa
    21661 tacagctgaa gccaaaacag cgctgacaaa aaacaccatc agcggattca caattctgct
    21721 caaaggatcg cgtggtattg gagttgaaaa tcttaaagaa attttgtgat gaaaaattac
    21781 gatattcacg aatggaatgc cggtgtaggc atgatgtcgg gcacatcgct cgatggtgtt
    21841 gatattgcca tttgcgcatt tcgattcaac aacggagttt gggaataccg gattgacgat
    21901 gctgttactt ttccatatgt ggatgaggta aaagcattaa ttctggaaat gcccggcatg
    21961 agcgggccag aactcatgat tgcagaccga aaacttggca aatatcacgg acaactactt
    22021 tccggatatc tgatggccat gggcgaaacc cctttatttg ttgcatcgca cgggcacacc
    22081 atctttcatg aaccctcgga aggcatgacg tatcaggcag gacacggagc cgccattgca
    22141 gcagaaaccg gcttaacggt agtctgcgat ttccgcagca gcgatgttgc attgggaggt
    22201 cagggagcgc cattggtgcc catcggcgac gaactgttgt ttggcgaatt tgatgcactg
    22261 gtaaatttgg gcggctttgc caatatatcc tatcgctccg acaaccaacg tattgcatac
    22321 gatatctgcc ctgtcaacat tgtatttaat cattttgccc atctttcagg acatgaattt
    22381 gaccgcgacg ggcaattagc ttcggctggg aaaccgtatt cggagctgct tcccatgtta
    22441 gataaaattc catactattc tgcacatcct ccaaaatcgc ttggaagaga atggatagaa
    22501 aaagaattta tgccagtgat tgaaaaaagc ggaattgtaa acacggatga catactatgc
    22561 acgttggctc atcatgcagc cgggcagatt gtacgcgcct gcggagcaaa caaatccatt
    22621 ctgtttacgg gaggcggggc aaaaaaccat tttctcacag gcatcattcg caaaaaattg
    22681 caggatcgca tgattatccc agatgaaaca acaatagatt ttaaggaagc attggttttt
    22741 gcattcatgg gttggttgcg tttaaatgaa atttcaaaca cactaccatc tgcaactggc
    22801 gccagcaaag caatcagcgc gggtgccgtt tttattccat aaaaaatact tgtggtataa
    22861 aatatttttt aattgcgcag cgttttaatt aacacagcgc tgttgacaat aatcaattgt
    22921 ctcaaaaaaa gcgaacagca taataagtta cttttacttc aaaatcacgt aatatgtttt
    22981 tgaatgttct tcttcagatt gttcagggcg gagattcgct tgcaacagcc accgccaaca
    23041 ctgtgcaaaa aaccgttgaa actgccaacc agcagtacga cacactatca ttctgggatt
    23101 tatcgttaaa aggcggtcct gtaatgattc ccatagcatt gcttctaata attgccgttt
    23161 atatattttt cgacagatac atagcgacga gtaaagcagc aaaagaagac agtggattca
    23221 tggccaatat aaaacatttc atcaccgaag ggaaaatcga tcaggccaaa gccatgtgtc
    23281 tgagcaatcc cacccctatg gcacgcatga tagaaaaagg aatttcgcgc ataggcaggc
    23341 ccttgaacga cattacaacc gccatagaaa acgaagggaa actacaggtg gcacggcttg
    23401 aaaagagcat cgcattttta tcaaccattg ccggaatagg accaatgatc gggtttctgg
    23461 gaactgttac gggtatgatt accgcatttt acgacctttc aaaatcggga aacaatcttg
    23521 atatagccct gctttcagga ggcatttatg aagcaatggt aacaacagta gccggtctta
    23581 ttgttgggat tctggcctcg cttgcataca atgtcatcgt tgcacgtgtt gaaaaaatcg
    23641 tttatatact cgaagcacga tcaacagaat ttatggacat tctgaatgaa ccagcataat
    23701 caaactaacc atgaatatca aaacaagaaa taaaagaaca acagatttca actctgcatc
    23761 catgtcggat ctggtgtttc tgttgctgat attcttcatg ctgacatcta cacttgtgag
    23821 tcccaatgca ataaaactgc ttcttccaag cagcacaagc gagcgggtac ctgttgagaa
    23881 aaaagcggtg aatgtttaca tcgacaatca gttcagatat tctatcgatc aaaaaatcct
    23941 agatgctgcc ggcttgcgcg aaggaatcaa atcgagactt gacgggcaaa ccgaagcgtc
    24001 ggttgtatta cgttccgatg caactgttcc cattcaattt gcagtaaatg tcattgatat
    24061 tgtaaattcc atcaacaaag aaaaaggaac aaaacacaaa gtcattcttg ctacacgccc
    24121 cgaagaaaac atataaattc atgaaatcga aaagaaaaat aacatcagca ttattaacac
    24181 tcgcgttttg cggaggtgtt gttgttatat tatttctgtt tgcatttcgt actccacttc
    24241 cattacccga agaagaaggc gttgttattg aaaccaatgc cggcggagga ggtggcggat
    24301 cggacgaatt tttcgatcag gaattctacg aagaaatgtc ggccgataat attggtgaaa
    24361 caaacgaaga ttatatcaca caggatgttg aaaatgtcaa ttatacatca gggcagcaaa
    24421 gcacaaacac cactgacaaa ccaaaagttg ataaccgcct gacaaatttc agctggggac
    24481 agggaaatgg tactggaacc ggtactggaa gcggttcggg ttcaggcaca ggcacaggta
    24541 gcggcacagg cgttggttct ggcgatggac ctggaacagg ccccggaagc ggtcccggct
    24601 attcgctgaa aggtcgggga gccaaaagta ttcctactcc taaatatacc gaagatgatc
    24661 agggaaaagt tgttgtaacc atctgggtgg atcgcgacgg aaaagtaaca cgcgcagaac
    24721 ccggcgccat cggaacaacc gtttcaaatc cttcgttatg gactgcagcc aaaaatgctg
    24781 cgttacaggc aaaattttcc acaaatgaaa gtgctcccga aattcaaaaa ggcactatta
    24841 catacacttt tatcaaactg aattaaaata gtttgtaaag tgaaaatgca ctttaatcat
    24901 taaattgaag tgcctttata aaccagataa actttctact ttccactctc tttgggagga
    24961 taatcaagcg aatagtgcag tcccctgctc tctttgcgct ccaacgccat tttaataatc
    25021 aaatagccaa ctgaaatcat attgcgcaat tcgcaaattt ctttagtaac taccgatttt
    25081 ttgtacaatt cttccgtttc ttcatacaag attttcaaac gatccaaagc acgcttcagg
    25141 cgcaggtttg aacgcacaat gcccacataa cttcccatga ttgaattaag ctctttacgg
    25201 gtttgtgtaa tcaaaaccat ttcttcgttc aatacagtgc cttccgcatc ccacacagga
    25261 atgtcattgt taatatcaat atttttaaca gcttcgatgg aatcaagagc cgcacgatga
    25321 ctgaatacag cggcctccag caatgaattg gaagcaagcc gatttgcgcc atgcaaacct
    25381 gtgcacgaaa cttcaccggt ggcatacaaa tgtttaatgt tgtttctgcc gcattcatca
    25441 acctttattc cgccgcactg ataatgggct gcaggtacta ccggtatcat gtcgcgcgaa
    25501 atatcaatac ccttgctaag acaatgtgca aaaatagtcg gaaaatgttc cagcaatttt
    25561 ttctttccga tatgccttgc atcaagatat aaatgatcgg cgccactgat cttcatttca
    25621 ctgtcaatgg cgcgtgcaac aatatcgcgc ggagccagcg ataaacgatt gtcgtatttc
    25681 tgcatgaatt cattgccatc gtatgttttc agaatcgctc cggcaccgcg cagggcctcg
    25741 gttatcaaaa atgcaggttt ctcggtcgga taataaaatg cagttggatg aaactgaaca
    25801 aattccatgt tttcaacaac ccccttggca cgatatacca tagcaacacc gtctccggta
    25861 gccacttccg gatttgtagt aacaccatac acatttccgc ttccaccggt tgcaatcaag
    25921 gttgtttttg aaataaaagt atccactttg ccggtttcag gattcagaac atacgcacca
    25981 aaacaagtaa tattctcaga tttcttcgtt acattttttc cgagatgatg ctcggtaata
    26041 atttcaatcg taaaataatt ttcgagaacc gaaatattcg gatgttcacg aatctgcttc
    26101 gtaagcgcac gctgaatctc ggctccggta cgatctttat agtgaaagat tcttttttcg
    26161 ctgtgaccgc cttcgcgcgc cagatcatat tctccatcag tatttttgtc gaaatgcgtc
    26221 ccgaagttaa tcagttctac aatacgttca gtgctttcct caatggtaat gcgaacaatc
    26281 atttcattat tgagtcctgc tcctgcaaca atagtgtcat taatatgctt ttcgtaatta
    26341 tcgggaccat acatcacccc ggcgataccc ccctgcgcat aatgtgttgc agtttcatca
    26401 agtaccgatt tggtaagtat aacaaccttc ccataaggag ctactttcag cgcataggtc
    26461 agaccggcta tgccactgcc gataatcaga aaatcgcaat attgcttcat ttattctgaa
    26521 gttgatgaaa tacaaaatta caaataaaac aggaacacta tctttgcagc gtgaaaagca
    26581 aaatctggat atacatcggg ctcatgttcg caatggctgt atgggcctat tcttatgtgt
    26641 tgaccaaaat tgtctattca tacggagttc cgcccaccat gctggttttc atgcgtctgc
    26701 ttctggcaac catcaccctg ctggctttta caaaaattac cggaaagctg caaaaaattc
    26761 aaaagcccga caagatctcg tttctggcac ttgcattctt cgaaccgttc ctgtatttca
    26821 taggcgaaag caatggatta atgctggttt cacctacagt cggatctgtc attattgcca
    26881 caattccagt attcataccc ttcgtattgt acgcatttta caaagagaaa gtagataaaa
    26941 cgaacttttt cggaatcatc atttcattta ttggagtgct gctcgttatt tttgaagatg
    27001 attactcact tgccgcatca cctgtcggat tggccttcat gttctttgcg gtattcagtg
    27061 catttggcta caccattgtc ataaaaaaac tgacccaaag atacaattca tttaccatcg
    27121 ttacttacca aacggtcatt ggcgctgtgt tgtatatccc tctgtttgtg ttttccgatt
    27181 tcagtgttat tgcaaatgtg gatttcacct ggcaaatgat tgcagcaaca attacactgg
    27241 ccatttttgc cacggccatc gcttatatac ttttttcaac tggaatccgc gaaataggcg
    27301 ctatgcaatc aaccattttc gcaaacttta taccggtgct aacagctcta ttggctctac
    27361 tttcagggat ggaagaattg cctatgcaaa aactcgcagg tgtactgatt gtcattctgg
    27421 gagtatttct ttcccaaagc aaaaagccct ttctcggcct gcccggaatg gtgtggaatt
    27481 attttgtgaa aaagaaatga gttgaatcat gaacagaatg atttaagaat aacgattgtt
    27541 gatcggtgat ttttgatcgg acgagcaaat ttgacatctg ggttcagatc taaaatcaaa
    27601 aatcttaaat cctctagttc cgtttgaaaa cacggaatgt tcttaaatca aacacttctt
    27661 cttccttcga agtttcttgt tcagtattca catcaagttc gtaaagttta taaagttcat
    27721 aaagtagaaa ggcactttaa attcttgcct taacttctca cttcgatgtt ccttgttcag
    27781 tgttcatttt tcacaagact tttgccgcca ttgataaaaa caacttcagc ccttcttctg
    27841 cggcagcatt catgcggtaa ttgatattat cagtcagata cgaaaccgca tcgtcaaacg
    27901 aaagatcagc tgaaccaaaa tggcgaactg ctgctgcagg atcttcaatg ccggcttcca
    27961 aagcggtttt caatggctta agcatttcat ccttcatatc ggcacgtgca atccaaaccg
    28021 caaaaacaaa tggaagtcct gtcatttcct tccacgcttc actcaaatcg aaactgtatt
    28081 tatagtcttt ctgcaattca aacgccttgt ctccaatagc aatacgcgca tcagccgagg
    28141 catcatcatg ttttacaaaa gcagggtgaa tgttccagtg attgtgacac agaattttca
    28201 gcaacaggtt cgaactgcgc gaatgactat caggcgaaat atgttttata tcctgcaaag
    28261 gccggttgct taaaagcaac accgaacgca caggcccatc ggcactgatg cagtatggtt
    28321 ttataatctg aaagttttca atcgaattca aggcgccaac aggaaccagc gctacatcgc
    28381 tgctgccttc ggcaaaattt ttcgcacatt gcgcaggatg aacaatactg agctgcgaat
    28441 acaagccgtc atcggcattc agcaatccaa aatgatatgg aaatgaattc aaataactta
    28501 caagagaaat cttcataata cacaaaatcg gatgcaaagg taacttttca tttccgcatt
    28561 catactacaa tcaccgatga tatttatcaa caataaaata ttatcttcgc atcgtcatta
    28621 atccaaatga aaacaaccat cacaatcaac cgtttagctg aatgggctca gaaattaagg
    28681 gcaggaacaa agtctttcgc ctcaggaaac cagcccgaag cattttacga agccatgact
    28741 cacaacaagt ggtttacaaa agaaaatatt atcgtctcga taaacgcgct tgcagaaatg
    28801 atttcggatg agaacacagt aaatgaatgg ttggcaaaat atccggagcc ctcatcagat
    28861 ccgaaaaaaa tattactgat tttggccgga aatattccgg cggtagggtt tcacgatgtt
    28921 ctctgcacat tactttcagg gcactctgcc gaaataaaat tgtcttcgga tgacaaattt
    28981 ttaattccat ggatgctcag aaacgcagaa aaatactttc cggaatggac ggaccgcatt
    29041 cattatcacg aaggcctgat tcagaatttc gatggggtta ttgccaccgg aagcaatcaa
    29101 tctgcaaaga cttttaaaag ttattttagt gcaaaacctc atattattcg ccacagcaga
    29161 agcagtattg ctgttataaa caacaatgac agcgacgaag atctgaaaaa actgggagat
    29221 gatgtgttca tgtatttcgg acttggatgc cgcaatgtat cattgatttt tctgccgcaa
    29281 ggcttcgata aacaaagact ttttgatgcc tttttatcgt ggaaggatct ggaatatcac
    29341 aacgcatttg ccaataacta tgtgtactac aaaggatatt tcagcctttc aaaagaagag
    29401 cccctcgatg ggtcatttta tctgttgcgc gaaaacttgt caacagctgc tcctgtagcg
    29461 gttttacatt atacagaata tacacacatc aacgatgtaa aaaaatacat tgctgaaaat
    29521 gaagagcata ttcagtgcat tgtttccacg caaaatgaaa tagaaaacgc tgtaaaaccg
    29581 ggtcaggcgc agtctccgcg cattgatgaa tatgccgatg gagttgatac gatggcgttt
    29641 cttgctgcgt tgtaagccat atcacggatt tcaaatccgc gctatggttg aaaaaaaatt
    29701 tgcaagcgta ccgaccttcc atagtccaaa atgaaatgaa ggacatggaa gagtcggctg
    29761 agtaaacagc catgtcacac atttcaaatc cgcgccaggt gtagagttcc ttttaaaatt
    29821 acattacaaa ttctatatta caagtcaggt ttgtgtaatt ttcttacttt tgtttataat
    29881 ctttagccat gaaactatcc agaaagtgcg aatatgcttg cttagcgatg atagaactgt
    29941 cggggcgtaa cccggatgaa tatacaaaaa ttgaatctat ttcaaacacc tggaatattc
    30001 cgagaaaatt tctggaacaa attctcatta ccctgaaaaa tgctggctat ctgcacagcc
    30061 gcaagggagc tgatggcgga tataaactgg ccaaagaatc ttctaaaata tccctggctg
    30121 aaatcatccg actgattgac ggagcgctgg ctccggttga aagtgtttcg acctatttct
    30181 acggctcttc gcccactgaa aagcatccgg gcttcatcaa catcatgaaa gatatccgcg
    30241 attatgtggc tgaaaagctt gaaaagacta cgtttgcgga tcttgtcagg aagtagtttt
    30301 cagcgccttg cttgagttca taaagttt
//
LOCUS       DAOPKE010000031        29332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1691_length_29332_cov_11.120060, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000031 DAOPKE010000000
VERSION     DAOPKE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_29332_cov_11.120060"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKY63_05805"
     CDS             complement(<1..941)
                     /locus_tag="PKY63_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00161.1"
                     /translation="MKIVFLGFVFVSIVAMKAGAQNLVIDPSMEAHYYVYDSLTSIDC
                     DSAGLFPTYWLSPNFGSPDYIHKNANCYNPTQWPYYCCVPINSRGVQNPKDGLAYYFL
                     GVFSYYNFYDTTRQEESLKGELFSELEAGKTYCISFYLSQADVFNLTMNQIGIYFSKT
                     NYFTPWPTIDSIPQLVIHFGEFFTDTANWVLFNGKFTASGGEKYVTFGTFESQKPYSF
                     GYIQATDSCTYPPCWATGCGIFIDMVSLYDCTGFYYEAEAGESRSLCKGEETTLGWDE
                     NTARSFKWSVVSGDSASLDNDSIPRPTVSPQKTTVYAL"
     gene            complement(997..4479)
                     /locus_tag="PKY63_05810"
     CDS             complement(997..4479)
                     /locus_tag="PKY63_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPF00162.1"
                     /translation="MRLFYSVNGSASDLNGLWYHITDITFPPGQVDVYWTPPCVNPDP
                     YFANQGIDYYYLGYTWVEKDNSDCNPTNMCAFYGTYSNPQAIQVINPQISISATPNPI
                     CIGECSNLTASGGVSYNWSTGQNSNPITVCPQAATNYNVTGTDSYGCTGTSSKTIIVD
                     YPKPDFTVSPNPVCLGNAINFFDESTCEESIVSWFWQFGDGSSATGQNTYHYYTSSGA
                     FQVTLTVTDSYGNTYTKTRTVTVRPQPDHIVLSGVFNDCEESKIYYTIDNAYMYDSYF
                     SWEIPAGSGIFFNNHQNTTTSSNTTQIIMWTTPLTQPAPIIITACDKYGCCITDTFYV
                     YPCCDKNIDLVFNDTPITGFSSGIVNGESIVINGTCEIPSTIEFQNCTFLMGGGAKLE
                     IPDGVSATFTTCNIAQCNRYMWEGIYLLTQNSEVIIQSNTVIKDAMKAVVSNSCGKYK
                     ILNSTFDLNYRNIVVNQCDQILLNEIHGSTFTCSNSSFMLFPHQGKRTYSAIEANKVR
                     MMHVGQPNNGIIDNTFSNISGGILLTNTSAEIKNNSFSGINIAPFQPNLPAIKVVNSN
                     FGLGTIQSYNMIVGGPNSVDGNIFTNCSKGIDIQYGRRSLIQNNRFSKTMVPVYFAFC
                     SKAAETNVLDNRITNASIGIYAYRNKNSKNTISNNTISWNGSGLMFPVPGTGIKIETF
                     LSNNEIYNVSFNDISRARVGIYTNSVAYSTLRENQIDVVAGSSILHSYGIRTLGGEKL
                     TVYGNSIDGPAQAANYSYGISMSMSGGSRIECNTVEKCHHGLNFDGNMPSEVYRNSMR
                     KNFYGVTFTNGGIIGPQGNTSYPSDNTWKQNTQDGWSFSSYGDQSLFHVRTSLPFQGG
                     NVAAYIPAFSITYYSPYPSNYTAPKCSTDAGGTIQMCQISLPGPSLPLGFKKQVAKDQ
                     ITYNMFPQSESWQAKKDVYGLLKRDTLFTYLTDTVLTQFVDSMMVSPVETQKSASVLF
                     SNDSVATAQLLNNASAANTLTEQSLKILNNIAFRADTVALAYTSAEISWLQFMAAQCP
                     YEYGPAVYMARAMLAPVDTTVYFNMCELESENNKSAETINEDVYESDIDVSVFPNPAN
                     DEFQIYAESTSREGSYCYYITDLSGTVVQQLSKGKYNAVEQVEVSDLSPGVYILCVIE
                     ESGLTHNYRLTVIK"
     gene            complement(5334..7754)
                     /locus_tag="PKY63_05815"
     CDS             complement(5334..7754)
                     /locus_tag="PKY63_05815"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPF00163.1"
                     /translation="MTNYCLLLLVLLMPLFSAAQVKDVTAVRTMSQPNIDGDISDECW
                     SLATPAKDFFQKSPFYNKPASQQSEVRVLYDDDGIYFAIRCFDSAPDSILRQLGTRDE
                     DLNADRFNIKIDPYNNHLDAFVFGVYASGPQADSRFQDEAFNAVWKSESRIDEKGWTA
                     ELYIPYSALRFPKADVQLWGIQFERLIRRSRETNQWALEEQNATNVQLLWGTMSGIQG
                     IDPPVRLSINPYLSASVSHFPHHDASVDDYSENFGGGLDLKAGLSESYTVDMTLLPDF
                     SQVQSDDIIKNLSAFEIQYEEQRMFFKEAVDLFEKGNLFYSRRIGRTPRGLYDVYNEL
                     DSGETVISNPSQAQLVNASKISGRGQNGLAVGFFNAITKNTYAEIRTRSGGTRKVLTE
                     PFANYNILVIDKEFKNSNSFYIINNNALRERLYQSSNVTGAGTGIYVKKKTFKFSFNA
                     ALTQNDTALFSDRAIKRAGLAHATSFSKVRGNWTWTLGQSTRNSRFDYDDMGIMSMNN
                     YQSYTFTSAYAWYNPAPWLKEIRMNNYLESSYRLSTGRSIGSEAMIRLSLLTMKHHFY
                     IWGGYEHSLSRAYNYYESRNPEMPYYEPHWSYAYLGFSSSYSKPFALDGDFSLVNVPY
                     DNSYAVNITLSPILKIGNRFQIRYEIGTEDTKRSVGYAGTDTLGLPLFGVRDVVTVNN
                     TLSLTYVIRNYMPITLRFRHYYNEGRYDRYMTLDENGEPFIAANGDYNNDFTFNAFNI
                     DFVYSWQFSPGSSLSLIWKTGIQTELLPTHMSYLQNFRHTLDSDQFNSLTLKVIYYLD
                     YQQVRRHK"
     gene            complement(7853..8488)
                     /locus_tag="PKY63_05820"
     CDS             complement(7853..8488)
                     /locus_tag="PKY63_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPF00164.1"
                     /translation="MRRIILTICITASVFSASAQSVIKLPDPVTTGGKPLMDALSERH
                     SGREFVDSAMSDQQMSNLLWAAYGVNRAESEKRTAPSARNMQEFDIYLALKKGLYRWN
                     SENNQLELLKAGDFRGRIGRQTFVASASLVLIFVADYSRMGKMEEASKDFYSAADCGY
                     ISQNVYLFAASENLNTVVLGSVERESVAMLLGLKSSQKIVFAQPVGFAKSE"
     gene            8562..9080
                     /locus_tag="PKY63_05825"
     CDS             8562..9080
                     /locus_tag="PKY63_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HPF00165.1"
                     /translation="MKKIRLIALIMFSMIASAGYSQMQFGLQVGPQFPLGDFSSGYNT
                     GFGFNLNGKYFATDDVAFGLNIGYHHFGTDWDDINCGFMPITLLAEYHLSTEEFQPFL
                     GCDLGVYNVSVNVLGIKDSDLQFGLAPYAGIQYQLNDNLGLTGSVKWNYVMTDNSDFD
                     WFGLNLGVVFGL"
     gene            complement(9184..10455)
                     /gene="rmuC"
                     /locus_tag="PKY63_05830"
     CDS             complement(9184..10455)
                     /gene="rmuC"
                     /locus_tag="PKY63_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPF00166.1"
                     /translation="MKDDFQRNREESAKLTKENRDELSNSLKDFKTEMSEALRLITEE
                     NRKVQEKLTTTVEQKLLLLTEQLVKNADQQREVLEKSIKGFQESFDKSVQSFNEVQRE
                     KFSQLENRQNELLVNTEKKLESIRLTVEEKLEKTLSERLGQSFETVGKQLLEVQKGLG
                     EMQSLAQDVGGLKKVLSNVKDRGGVGEIQLEMLLEQILAPEQFDSNVKTKLGSNDLVE
                     FAIKLPGKDDMNSVVYLPIDAKFPRDVYEKVVDAFETADTNVIDAANKDLENTIKKMA
                     KDIRDKYIDPPHTTDFAIMFLPFEGIYAEVIRRSNLLEQLQREFKVIVTGPTTLAAIL
                     NSLQMGFRTLAIQKRSSEVWKILGAVKTEFEKFGGLLEKAQKQITSAGETIDQLKGTR
                     TRAIQRQLRDVQVLPGTEDTQVPELPAGEDE"
     assembly_gap    10465..10564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10566..11447)
                     /locus_tag="PKY63_05835"
     CDS             complement(10566..11447)
                     /locus_tag="PKY63_05835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF00167.1"
                     /translation="MKSTSKPLQFTVKNADTLLPFLTKVFPGKPRTGLKQMLQGGRVT
                     IDGKTVTRHDFILNAGMLVVIGSPVSKPATAIPGIKILYEDDSIIAIDKPSGLLTVGT
                     EKEKTNTAYSLLSHYVKTENKNNKIFIVHRLDRDTSGVLVFARNTMVRDVLQEYWHNE
                     NHKREYAALVCGKIEEPRGRIESWLKENANLQVFSSLRPDDGQHAVTNWELVQQNENF
                     SLLKVLPETGRRNQIRVHLYDIDFPIVGDRRYGNGQNPIGRLGLHATLLAFVHPVSGK
                     VMQLESPVPGSFFRMVK"
     gene            complement(11577..13793)
                     /locus_tag="PKY63_05840"
     CDS             complement(11577..13793)
                     /locus_tag="PKY63_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HPF00168.1"
                     /translation="MKKLLTFSFWIFMAFGTFAQSGVLYPADDVLLKNAVATDPELID
                     RYVAYEADFETLMNSAKTDTLIGGKRIIPVVFHIIHTYGAENISDDQVYDALEKLNTD
                     YNLQNADTADIFPLFKSRAANCQIEFRLARIDTNGNCTSGIIHHFDPQTNFAYFNTMA
                     KYCWTPSKYMNIFAVNFIYPEGMSLPDGAFIGGMSPFPPSNTLTQALTGGDTLMDGVL
                     IRQDCIGTIGTAADMAGMGLNLMNRTFTHETGHYFNLYHPFQSMYAALGIDGCGMPPL
                     FETGDEVDDTPPVAAATQNTSLSCFVPGSINSCTTDSPDEPDMIENYMDYQWGYCTNM
                     FSLGQLDRINTTLMNDRRNLWSYENLVATGVFDTNAVLCAPVADFHAEAYTICAGNTV
                     TFHDVSYNGAVASRTWSFAGGSPSTSTDQTPVVTYAAPGVYAVTLKVYNATGSDSLTR
                     VSYIHVMDPSVVTVAPLIESFETINFNSDLTIHNDTGAAWAQSASACTGAKAIYLNNF
                     DGNVAGSYDAFITPAYDLSGLGEHTKLQFRCAYAPKFVAGSLLAAADTVYDKLTVYFS
                     NDCGETWVNKLSLTGNALASASPAETAFNPASEAEWGTHEIILPQGTLSGYANLRVKF
                     EFYSNGGNNVFIDDINIHPNSWFGVNESIVDNSFAVYPNPAGDETNISFTLHDNANVK
                     LSVLDITGREITVITTSNLDAGAHRFPLSLNQVKAKGTYFIRLEVNGMQMVKTVVF"
     gene            complement(13828..14577)
                     /locus_tag="PKY63_05845"
     CDS             complement(13828..14577)
                     /locus_tag="PKY63_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF00169.1"
                     /translation="MLWWYYPLLFATGLVVGFVNTIAGSGSFLSLPVLIFTGMPANVA
                     NGTNRISILLGSLTGVLSFSRKKYFDVRNALILAAFTVPGAIAGSFFVVEIPDEFVEK
                     FIGYVMIVFAVLTIWKPRAFSRKPAQNVSFTIKWYHCLLFVAIGFYGGFVQAGVGIFM
                     LFALVLSIGYDLFNANSLKLALTLVFTPFSLAVFIYNNQVDWIPGLVLAAGSIIGAVF
                     GAKTALKRGAGFIRVVLFAVLLISAAKLIFS"
     gene            complement(14760..17231)
                     /locus_tag="PKY63_05850"
     CDS             complement(14760..17231)
                     /locus_tag="PKY63_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00170.1"
                     /translation="MLRFSFLMVVLSLMVFSFGFAQPGNMPDANFQITGIVLDSVSGK
                     PVEYATVALVSMRDSSIAGGGITNAMGEFSFVSKTPGRFMLRINFMGYANKSVGPVQL
                     KPGSGPLFDLGKILIAPSAQSLSGVDIVAEKPMIELSIDKKVVNVAQNLNTSGGTALD
                     VLKEVPSVEVDEDGNVSLRGSENVTILIDGRPSTMTGADRRAAMEQIPASSIESVELI
                     TNPSAKYSPEGMTGIINIILKKKKGTGLNTLLSLNAGTKDKYSASFSASYSTDKWTLF
                     GNYNYQNRKRTVTGESERITYDLIESYFLRQDIDEIHGNQSHNIKFGGEYHFTPRFIV
                     AASASTFIFSDREDQKTMNYTMNPENIYTQIYSNNSLENASMNNWDANLNIKKRFTKP
                     KHEIILDFSMSEGNRKDSSFNRITFLEEDFATVTSEIPEWSKTVAPNLNSVKTLKLDY
                     VYPFNDSTKLEAGFDGSMRSIDADSRYYNYDFGGSGWVFNDTTSNHFLFNENIAALYA
                     NYSTKIKKWGLSIGSRIESASTNAREADSLDNRKTYYSLFPTGALSYKISKVQEIQLT
                     YSRRINRPSFRDLNPYRDYSAWPNIRTGNPFLDPEYVNALEMSYAWYSKRGTFMPTVF
                     YRRITDVISRYRMNLNDSVYLMMPENYTSATSYGFEMIYTHKPLKWWNTTISGSWYRH
                     IVDGSNVETSITAAAFGWQLRNSNSFRFAKGWEGSFSVFYRSPRETGQGIRDAMIMAD
                     CAVKKSFLNDKLAISLNVRDLFNTMKFRMDFMEADYEMYMQRKMEGRIFMLGFTWKLS
                     GDYKSKEKRSNANGNGGNDVDDGGF"
     gene            17439..18167
                     /locus_tag="PKY63_05855"
     CDS             17439..18167
                     /locus_tag="PKY63_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00171.1"
                     /translation="MIKNISFFAVIIILAMTSCKDDKTEYTPFEPVNNQAAHTSAYYN
                     AGLGDNPGVPSGTLYSFPSGIEIIGYIHGNAPTVLSAPVFDKIKNPGSMPDYSDPKLN
                     YLSLGYGSLVNLYFSVYNNTNTQKSVSIPKGTICYEANHTPADQHGILVKSVSFNVPA
                     HDTTDVHLALFCVNAHHGIATATDPYKIGVITIHPDLITVCNILDAKPTIDPNDYGTI
                     QSILWKITDNSGFGQADIDTLNAM"
     gene            complement(18298..19704)
                     /locus_tag="PKY63_05860"
     CDS             complement(18298..19704)
                     /locus_tag="PKY63_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPF00172.1"
                     /translation="MSGFFGSVSLGDCVNDVFYGTDYHSHLGTKRAGLAFYSKEAGFQ
                     RAIHSIENSYFRNKFEDELPAFNGHIGLGVISDTEAQPVVMTSHLGRFAVATVSKIVN
                     IKELEKRCIDKQYGFSEMSMGNINPTELVAKLIAEEKDFVSGIRNVQELIQGSCSLMV
                     MTSDCIYIGRDKLGRTPIVIGKKGGAFAASFETCAFPNLGYETFHYAGPGEIGKITAD
                     GYTKLKEPNDQMQVCAFLWVYYGFPTSSYEGINVDECRHRCGEALAKNDSVNADFVAG
                     IPDSGVGHATGYANFTHIPYKRPYSKYTPTWPRSFMPQSQAQRDLVATMKLIPNTAVI
                     KNQSGVFLDDSIVRGTQLKDNVKDLRKAGIREVHMRIACPPLTYPCPFLNFSRSRSNM
                     DLATYIAMHELEGKEERDMAVYSNPDSEEYKQMVERIRLRLGLDSLKFQRLEDLIEAI
                     GLPKDKICTHCFNGSSYF"
     gene            complement(19962..20513)
                     /locus_tag="PKY63_05865"
     CDS             complement(19962..20513)
                     /locus_tag="PKY63_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00173.1"
                     /translation="MKILKFVAVAFLAFLISSCLTVEKKKYKFEITGKNTGKLTITYV
                     NIFSQMDDSTDVSEQDFDELINDYLNGEKFADNFPLASDIQKRLYEENGKLNGEVTMN
                     FASLEAVRLYQMNKKSPICFNVGQTIDSESFESTNGTYGNKDFMNVIFWTPKTKVLEL
                     TTTVNEYNEDDCVSLIKQYRRWK"
     gene            complement(20603..21655)
                     /locus_tag="PKY63_05870"
     CDS             complement(20603..21655)
                     /locus_tag="PKY63_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF00174.1"
                     /translation="MKKNTGVLISPLDWGLGHAARCIPLIQQFIAEGQAVTVFASPGI
                     CSYLQQRFPDLSFITDKTKPVSYGFNGTGFFQIIGLIPKMIKQQKSEQNMCSALCASN
                     EYNLIVSDNRYGFRCDNIRSVLITHQLEPIAPWWLCFGLPVLRKFIKKSLARFSEVWV
                     PDYKNFPGLAGKLSHPKTLVSNAKYIGPLSRFDRLDDYNSTVRNELLLVITSGPLKHR
                     RQMAGKLSQVFAECGLSVIIAGAEINDPGENISCIMSPSDEQLAKWLSEAAVIISHAG
                     YSTMMDLFAVGRSAIIFPTRGQTEQQYLAQLHQDFFITVANINKLHLHTAGWTSINEI
                     LKQKEIRMVHANNLKR"
     gene            complement(21660..24002)
                     /locus_tag="PKY63_05875"
     CDS             complement(21660..24002)
                     /locus_tag="PKY63_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00175.1"
                     /translation="MKFFTPFVLLMLIFPFYGFSQQTILKGTVTDSETGLTLPGVNVV
                     VNKTTGVATDIDGNYSLTLDKGTHSIAFRFVGYTEEIRLINLKEGETLVLDMKMTTES
                     RILDAVVVSAGKYEQKLSDVTVSMEVIKADFIRNTNVSSLEEGLQKIPGLNIMDKQPS
                     IRGGSGYSYGAGSRVLFLVDDMPMITGASGEARWDFAPIENVQQVEVIKGASSALYGS
                     SALNGVINYRTAYPGVVPKTYITSIFGQYTTPERKEIAWWGNNAPIFGSTRFLHSRQA
                     GNFDITFGGFVSSNNGYRTNNEEERYRLNGSIRYRDKKVKGLSYELSANYMRRIGDIF
                     LLWADGDSGVYKCNESFQQEVNNSYVNLYPSVIWYKNDSTKHSLKGRFFQVHNTNNTG
                     QDNFDDMYYGEYNFYKGYPSKSLNWTNGLCANYNESVAQIYGDTRHFGSSIGFFTQAD
                     KKFNRLSVSLGARIEGYRIDEDDMKFKPVFRSGVNYSVTEQSFLRASFGMGYRYPTIA
                     ERYTATSTGDLKVFPNPNLQPETGYSTEIAFKQGFRLWGWNGYADVAGFYTRYENMIE
                     FMFNYHNPDSVILIPFPPTSPNYFLNWVGFKAENVRNAEISGFEIVVTGEGKFIGLPA
                     TLLLGYTYNNPIDLDATADSMKSTKDNILKYRFYHSAKADFSVEYKKLTLGANFEYQS
                     HIINIDKIFEDTVRGPDGTTPLFNPATGEYAMILPGLKEYREKNDKGFLVFDMRIGWR
                     INDMIHVNFTAKNIFNREYMMRPGDVQPPRTFIIQLSLKV"
     gene            24106..25215
                     /gene="dprA"
                     /locus_tag="PKY63_05880"
     CDS             24106..25215
                     /gene="dprA"
                     /locus_tag="PKY63_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPF00176.1"
                     /translation="MNNKLLYLIALKCIPQVGDIVARRLIETTGDASVVFNMTDEKIE
                     KLPGLTPKTIRMIRENRTKALQRAENEIEFIDKHKIQCLTSFDDTYPSRLKHCLDAPL
                     FLFYRGASAVESDYMTAVVGTRKSTVYGRKMTEQLVSGLSLKGMTIVSGLAYGIDTVA
                     HQTAVDCNIPTIGIMAHGFKHLYPEENRKLATLMLKNGGLMTEFLSDELPEKQNFPIR
                     NRIIAGMADAVVVVEAQSKGGALITAEIANSYSRDVFTIPGRVDDQKSRGCNWLIRTQ
                     RATLIESAEHLRYFMNWTQAEKPKIRQVRMEVLLDAGAQSVYDYLFEHGEKPIDSICN
                     DLNISINTAAMILLELEFQGVVAALPGKRYRLKNY"
     gene            25306..26208
                     /locus_tag="PKY63_05885"
     CDS             25306..26208
                     /locus_tag="PKY63_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00177.1"
                     /translation="MKKLLLSSSLIIAFAISLSAQVNFTQNFDTCTAGDYAAECIGAP
                     WTTWSGTVGTAEDAVVSATQSSSAPNAAYIGSTSTDFVVLLGDLTTGVYDISFDFFVE
                     NGKMGYFNIMNSFTGGTYLWATDVYVRSTGYLSWTADNVSDSIPFAFDTWTKIGYTID
                     LNNDTAFMYVDDSLVNSWKFSKGTLGTGTTLKLAAMDFYGAAEDLQPGYYIDDFSFAI
                     NTTGIVENENNVSLYPNPATNAITIEGQANNRFEIFNTGGQMLLSGILNNNSETIDIS
                     TLNAGCYFIRCIGNDVTVNQFIVE"
     gene            26316..28169
                     /locus_tag="PKY63_05890"
     CDS             26316..28169
                     /locus_tag="PKY63_05890"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00178.1"
                     /translation="MKQIFLSFALFIICAVTLQAQVIISQNFDALTAGAGTYAAQQLG
                     APWTTWSGTTGNAEDPAVMTTQAASAPNSVYIATTSNDFVCHLSDKTTGRYKLNFQIF
                     VEAGKIGYFNILNDFAGSSSIWAMQAYFKSNGYCVVDAGAASADSLPYTTNAWHEVLF
                     IIDVDDDFATMYLNGTELVSWVFSGGSFGDGTTHKLDAVNFYGWSDDLQPGYYIDDFN
                     FEQVTVPEAPMNLTAAVTGSDVALAWTAPTSSPDSYTILRNNTVLASGITTLTYADNN
                     LYPQDYNYVAKAHYNNLGYSHSSNDTTITIAGGITRNNVLFEATTSTYCTYCPGVALA
                     MEEFVANGKNVVIVEYHNDWQGPDTYVSTASQARAVAYGSAGIATGNPTTIADGDLYL
                     VGGNHTISLYPNLLPMYDARIARNAIHDIDLSVVKTGVDTYEATIIVEQISSYYPGNL
                     YLRTALTESNIAFPWQGQTELHWVCRDMYPNATGTALDFSSVSTQTYTTTFSTTGYVA
                     DNCEFAAWVQLGATGDVTQAIKVDLSTVIGIEETNNSLWSIYPNPANDQITIAGIEKA
                     QYEIVNIQGQVVLSGTIENNTETINTSNLNQGSYFVRIVGEDVMVKSFVIE"
     gene            28330..28566
                     /locus_tag="PKY63_05895"
     CDS             28330..28566
                     /locus_tag="PKY63_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00179.1"
                     /translation="MTTVELKKLLIQRIAEINDVSFLKAVKTILESKTNTEILLLTPE
                     QKKEILESKKNIEQGLIIDNDSLDKEVAKWANAR"
     gene            28548..28850
                     /locus_tag="PKY63_05900"
     CDS             28548..28850
                     /locus_tag="PKY63_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF00180.1"
                     /translation="MGKRKIIWSDRAKKKLFEILEFYEQRNQNKTYSIKLYQRFKKEL
                     SVLPKYPDIGIKTEIESVRGLIVDDFILFYEPDKEKIIVHTIWDCRQNPDDLKIQQ"
     gene            28922..29188
                     /locus_tag="PKY63_05905"
     CDS             28922..29188
                     /locus_tag="PKY63_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00181.1"
                     /translation="MALLILLIVAWIVIIGYEAKNTGVNAFLWIATAIISYAAFSVTA
                     FFVFISFSPCNPEECKPVLYGMLAGNIMPLSIAVYLFFKKPNNK"
BASE COUNT         8870 a         6391 c         5701 g         8270 t
ORIGIN      
        1 agcgcataaa cagtggtttt ctgagggctc accgttgggc gtggaatgct gtcgttgtct
       61 aagctggcgc tatcgccact caccacgctc catttgaaag agcgtgccgt gttctcatcc
      121 cagcccagcg tggtttcttc tcctttgcac aggctgcggc tttcgcccgc ctcggcctcg
      181 tagtagaaac cggtgcagtc gtaaagggag accatgtcaa taaaaattcc acaccctgtt
      241 gcccagcatg gaggataagt gcaactatca gttgcttgta tatacccaaa tgaatacggt
      301 ttctgcgatt caaaggtacc gaatgtaaca tacttttctc cgccgcttgc cgtaaatttt
      361 ccgttgaaca aaacccagtt ggctgtatct gtaaaaaatt ccccgaaatg aataacaagc
      421 tgtggtatac tatctatggt cggccatggc gtgaaataat ttgttttact aaaataaatg
      481 ccgatctgat tcatagtaag attaaaaaca tcagcttgag ataagtagaa actgatacaa
      541 taagtttttc cggcctcaag ttcgctaaac agctctcctt tcaatgattc ttcttggcga
      601 gtagtatcat aaaaattata ataactaaat acacccaaaa aataatatgc taatccatct
      661 tttggattct gaacacctct agaattaatt ggtacacagc aatagtatgg ccattgagtt
      721 ggattatagc agtttgcatt tttatgaata taatcggggc tgccaaaatt tggagaaagc
      781 caatatgttg gaaacagacc ggccgaatcg caatctatac tagtaagcga atcatacaca
      841 taataatgtg cttccattga aggatctata actaaattct gagcacctgc tttcattgcc
      901 acaatagaaa caaatacaaa tccgagaaaa actattttca tcaacttgtc attttaaaat
      961 agagcagcga ggaatactcg ctgctctaaa attaaactat ttaattactg tcaatctata
     1021 attatgtgtt aatcctgatt cctcgatcac acaaagaata taaactccgg gtgataaatc
     1081 agaaacctct acttgttcaa cagcattgta tttaccttta gatagctgtt gtaccacagt
     1141 accactcaaa tctgtgatat aatagcaata acttccttcc cggcttgttg attcagcata
     1201 aatctgaaat tcatcgttgg ctggattggg aaaaaccgaa acatcaatat cactctcata
     1261 aacatcctcg tttattgtct cagcgctttt gttattttca ctttccaatt cacacatgtt
     1321 gaaataaaca gtggtatcaa ccggggctag catagcccgg gccatataca cggccgggcc
     1381 atattcatac gggcattgcg cggccatgaa ctgcaaccat gatatttctg ccgaagtgta
     1441 tgcaagagca actgtgtcgg cccggaaagc aatgttgtta agtattttca ggctttgctc
     1501 ggtgagggtg ttggctgcag atgcgttgtt gagcaattga gcagtggcaa cactgtcgtt
     1561 cgaaaagaga acggatgccg atttctgagt ctcgactggc gaaaccatca tgctatccac
     1621 aaattgggtg agcacagtat cggtaagata agtaaacagc gtatcccttt tcaataagcc
     1681 atacacatcc tttttggctt gccacgactc gctctgcgga aacatgttgt atgttatctg
     1741 atccttggca acctgctttt taaatccgag cggtaatgat gggccaggca aagatatctg
     1801 acacatttga atggtgccgc ctgcatctgt gctgcatttc ggagcagtgt aatttgacgg
     1861 ataaggtgaa taataagtga ttgaaaaagc aggaatgtaa gcagccacat taccaccttg
     1921 gaacggcaaa gaagttctaa catggaaaag tgattgatca ccatatgaag aaaagctcca
     1981 gccatcctgg gtgttttgtt tccatgtgtt atcgctgggg tagcttgtgt ttccctgtgg
     2041 cccaatgata ccgccattgg taaaagtaac accatagaaa ttttttcgca ttgaattgcg
     2101 atagacttct gaaggcatgt tgccgtcgaa attcagcccg tggtggcatt tttcaacagt
     2161 attgcattca atccgtgagc caccgctcat gctcattgat ataccatagc tgtaattggc
     2221 tgcctgcgcc ggtccatcaa tgctgttgcc gtaaacggtc agtttttcac cacccagtgt
     2281 tcgaatgcca taactatgca aaatgctgct tcctgcaaca acatcaatct ggttttcacg
     2341 caaggttgaa tatgcaacag aattggtgta aattccaact cttgctcggc tgatatcgtt
     2401 gaaagaaacg ttgtaaattt cattgttcga aagaaacgtc tcaattttaa ttccggtacc
     2461 tggaacagga aacataagtc cacttccatt ccatgaaatt gtattattgg agatagtgtt
     2521 tttactgttt ttgttgcgat aggcatatat accaatcgaa gcattggtga tgcgattatc
     2581 aagcacattt gtttccgcag ctttagaaca gaatgcaaaa taaacgggca ccattgtttt
     2641 tgagaaccgg ttattttgaa taagtgatct gcgcccatac tgaatatcga tgcccttgct
     2701 gcaattggta aaaatattac catcaaccga atttgggcca ccgacaatca tgttgtatga
     2761 ttggattgtt cctaatccga aattcgaatt tacaactttg atagcaggta agtttggctg
     2821 aaatggcgca atattgattc cgctgaaact gttgttttta atttccgcag aagtgtttgt
     2881 gagcaaaatc ccaccgctga tattagagaa ggtgttatca atgataccgt tgttgggttg
     2941 gcccacgtgc atcattctca ccttattggc ttcaatggcg ctgtaggtgc gttttccctg
     3001 atggggaaaa agcataaaac tgctattgct acaagtgaaa gtagagccat gaatttcatt
     3061 cagaagtatc tggtcgcact gattaacgac aatatttctg tagtttaaat caaatgtgct
     3121 gtttaaaatt ttatattttc cgcacgaatt gcttactaca gccttcattg catctttaat
     3181 cacagtattg ctctgaatga ttacttcact gttttgagtc aacaaatata taccttccca
     3241 catgtacctg ttgcactgag ctatattgca agtggtaaaa gtagccgata cgccatcggg
     3301 aatttccagt ttggcacctc cacccataag gaatgtacaa ttctgaaact caatagttga
     3361 aggaatctca caggtcccat taataacaat agattctcca tttacaatgc ctgaagaaaa
     3421 gccagttata ggcgtatcat taaaaacaag atcaatgttt ttatcgcagc aaggataaac
     3481 ataaaacgtg tcggttatac agcatccata tttatcgcag gcagtaatta taatcggggc
     3541 tggctgtgtt agtggcgtag tccacataat aatctgtgta gtatttgaag aggttgtcgt
     3601 attttgatga ttgttgaaaa atattccgct gcccgcaggt atttcccagg aaaagtacga
     3661 atcgtacata tatgcattgt caatcgtgta atagattttt gattcttcgc agtcattaaa
     3721 gactccagat aaaacaatat ggtctggttg tggtctcaca gtaactgtcc gcgtctttgt
     3781 ataggtattc ccgtatgaat cagttactgt aagtgtcacc tggaaagcac ctgaacttgt
     3841 ataataatga taagtgtttt gacctgttgc agacgaacca tcgccaaact gccagaacca
     3901 cgaaacaatg ctttcttcgc aggttgattc atcgaaaaaa ttaatggcat tacctaaaca
     3961 aacgggattg ggtgatactg tgaagtctgg tttgggataa tcaacaataa ttgtttttga
     4021 cgacgtgccg gtacatccat agctgtcagt tcctgtaaca ttgtagtttg ttgcagcctg
     4081 cggacaaacc gttattggat ttgagttttg gccagtactc cagttgtaag acacaccccc
     4141 tgatgccgtc aggttactac attcgccaat gcaaatggga tttggagtag ctgaaatgga
     4201 tatttgggga ttaataacct gtatagcctg aggatttgag tatgtgccat aaaaagcaca
     4261 catgtttgtt ggattgcaat cagaattgtc cttttcaacc caggtgtatc ccaaataata
     4321 ataatcaatg ccctgattcg cgaaatatgg atcaggattg acgcaaggtg gggtccaata
     4381 cacgtctact tgtcctggag gaaacgtaat gtctgtaata tgataccata aaccatttag
     4441 atcacttgca ctgccattta cgctataaaa aagtctcata caaccttcat gactgaatat
     4501 tgaattatca gtctgcgtag tatttaacgt tatatggagt gtctgaccac agcaaggtga
     4561 ataatcattc ggttcaagaa aaatttgatt gggatagtat gacattgcat ttttcaaata
     4621 cgcatagaaa ctaaaagtac ttgaattgct ttttagaatt ttcacaaagt attcattatc
     4681 ttttgtcaag cctgataatg aaattacatt tgataacgga gaaatatgaa ttggtaaaga
     4741 attacagttg ttttcataaa catacacact tccagcacaa gaatcctcat atgctaaaac
     4801 aagatttaca ctatccaatt cagccgtaaa agataaataa atgcatttgt ctgattccga
     4861 catcaaaaac ttttgatcac agtttgaaga ataaaaaatg ctcatggcgg tttgacaact
     4921 cgtctgtcca tctacattcc ccaacggcac tataaacaaa atcatcaaca ataaaagaaa
     4981 cttacaattc cggaacaagt gcagttgcgt gttttctgtg ttctcacaac tcttcgctct
     5041 cgctctcact ctcacaccga tttcaggggg ggtaattccc tgaatgacag ctctttacaa
     5101 gactgccacg ctgatacttt cataaagatt ttcatagttt ataaggttta gtagggcaaa
     5161 tctataaatt tatttataat atatgttggt ttaatggaaa taattttaat caagtcagtg
     5221 aagataaaaa ttatccagat aaaataaata attatgcgtc tttaatgttt aattacagga
     5281 ttgtcttttt gccagcaaaa atgagaaaag agaaaactga aacaaaacca gatttactta
     5341 tgcctccgca cctgctgata atcgaggtag taaattactt tcagggtaag tgaattgaac
     5401 tgatcgctgt caagggtgtg acggaaattc tgcagatagc tcatgtgggt tggcaacaat
     5461 tccgtttgaa taccggtctt ccagatcagg ctcagagagc tgcccggaga gaactgccac
     5521 gaatacacaa aatcaatatt gaatgcattg aaagtgaaat cattgttgta atcaccgttt
     5581 gcagcaatga atggttcacc gttttcatca agtgtcatgt agcgatcgta tctgccctca
     5641 ttgtaataat gcctgaaacg caaagtaatt ggcatgtagt tgcggatgac ataggtaagc
     5701 gaaagcgtat tgtttacagt cacaacatca cgaacaccaa acaatggcaa tccgagtgta
     5761 tcagttccgg cataaccaac gctgcgtttg gtgtcttcag tcccgatttc atatcgtatt
     5821 tgaaaacggt taccgatttt cagaatcgga gacagcgtaa tattcacagc ataagagtta
     5881 tcataaggga cattcactag agaaaaatca ccatccaacg caaatggctt gctatacgaa
     5941 cttgaaaaac caagataagc atacgaccaa tgcggctcat agtaaggcat ttcagggttt
     6001 ctggattcat aataattgta tgcgcggctt aatgaatgtt cgtatccgcc ccagatataa
     6061 aaatgatgtt tcatagttag caaactgagg cgtatcatag cttcactgcc aatggagcga
     6121 ccagtactca gacggtacga agactcgagg taattgttca tccggatctc tttcagccat
     6181 ggagccggat tataccatgc atatgccgaa gtgaaagtgt agctttgata attgttcatt
     6241 gacataatac ccatatcatc ataatcaaag cgactgttcc gggtgctttg acccaatgtc
     6301 caggtccagt ttccccgcac tttcgaaaac gaggtggcat gagccagtcc ggcacgttta
     6361 atggcgcgat ccgaaaacaa tgctgtatca ttctgcgtaa gggctgcatt gaacgaaaat
     6421 ttaaacgttt tcttttttac ataaatcccg gtaccggcac ctgttacatt actgctctga
     6481 tacaggcgtt cgcgcaaagc attgttattg atgatataaa atgaattgct gtttttgaat
     6541 tccttgtcga tgaccagaat gttgtaattg gcaaatggtt ccgtcagaac tttacgtgta
     6601 ccacctgatc tggtgcggat ttccgcatat gtgttctttg taatggcatt gaaaaatcca
     6661 acagccaatc cattctgtcc tctgccggaa atttttgatg catttacaag ttgtgcctgc
     6721 gaaggatttg aaataactgt ttcgccggaa tccaattcat tgtaaacatc atataaaccg
     6781 cgtggagtgc ggcctatacg acgggaataa aacaaatttc ctttttcaaa aagatcaacc
     6841 gcttctttga aaaacatccg ctgttcttcg tactgaattt caaaagcgga aaggtttttt
     6901 atgatgtcat cgctctgaac ctggctgaaa tcgggcaata gtgtcatgtc aaccgtataa
     6961 ctttcactga ggcctgcttt gagatcgaga ccaccgccga agttttcgga ataatcatca
     7021 acactggcat cgtgatgcgg gaaatgcgaa actgaagccg ataaataagg attgatggat
     7081 aatcgcacag gcggatcaat gccctgaatt ccgctcatgg tgccccacaa caattgaaca
     7141 ttggttgcat tctgctcttc gagcgcccac tgattggttt cgcgggaacg acgaatcaac
     7201 cgttcaaact gaatccccca cagctgaaca tcggccttcg gaaaacgcaa tgcactatac
     7261 ggtatataca attccgcagt ccagcccttc tcatcaatac gcgattcgct tttccagaca
     7321 gcattgaatg cctcatcctg aaaccggctg tctgcctgcg gtcccgatgc atacacacca
     7381 aacacaaatg catcgaggtg attgttgtat gggtcaattt ttatgttgaa gcgatcagca
     7441 ttcaagtctt catcgcgcgt gcccaactga cgtaaaatgc tatcgggggc actgtcaaaa
     7501 cagcgaattg caaaataaat cccatcgtca tcgtaaagga cccgcacctc ggattgctgt
     7561 gaggcaggtt tgttgtaaaa agggcttttc tgaaaaaagt cttttgcagg cgttgcaaga
     7621 ctccagcatt catcgctgat gtcgccatca atgttaggtt gcgacatagt cctgacagca
     7681 gtaacatcct ttacctgggc agcagaaaat aaaggcatta ataaaacaag cagtaacagg
     7741 caataatttg tcatccgacg ctaaattaaa taatttgcgt ttgcatcaat cagctttacg
     7801 ctcattacgt caaatatccc taccaaccgg aagaatttca agatatttca aattactctg
     7861 actttgcaaa tcctaccggt tgcgcaaaaa caattttttg agaagatttt aatccaagca
     7921 gcattgcaac cgattcacgc tcgacagagc caagcactac cgtattcagg ttttcggaag
     7981 cagcaaacaa atatacattc tgactgatat aaccgcaatc agccgcgctg taaaaatctt
     8041 tcgatgcttc ctccattttg cccatacggg aataatcggc cacgaaaatc agtaccagtg
     8101 aagcggaagc aacaaaagtc tggcggccaa ttcttccccg gaaatcacct gcttttaata
     8161 attcaagctg attgttttcg gaattccagc gatataatcc ttttttcagc gccaggtata
     8221 tatcgaattc ctgcatatta cgtgccgacg gagctgttct cttttcgctc tcagcgcggt
     8281 ttaccccata ggccgcccac aacagattac tcatctgctg atcactcatg gcagaatcca
     8341 caaactcacg tcctgaatga cgttcggaca aagcatccat cagtggtttt ccgcctgtag
     8401 tcacaggatc agggagttta attaccgatt gtgccgaagc agaaaatact gatgccgtaa
     8461 tacaaattgt caatattatt cttctcatct ttttttttgc taaaatacaa aaaacacaaa
     8521 ttaaaaaaat tgctaatatt gcaatataaa aaaacaacta catgaaaaaa atcagactta
     8581 ttgcattaat catgtttagc atgattgcaa gcgcagggta ttcgcagatg cagtttgggc
     8641 tgcaggttgg gccacagttc ccattgggtg atttcagctc aggttacaat accggatttg
     8701 gattcaacct gaatggaaag tattttgcaa ccgacgatgt cgcgtttgga ctcaacatcg
     8761 gctaccatca tttcggaacg gactgggatg acattaattg cggttttatg ccaattacac
     8821 tgcttgcaga gtaccattta tcaacagaag aatttcagcc gtttcttggt tgcgatctcg
     8881 gcgtttacaa tgtttcagta aatgtgctgg gtattaagga ttccgatctg caatttggcc
     8941 ttgctcctta cgcaggaatc cagtatcaac tgaacgataa tctgggactg acaggaagcg
     9001 ttaaatggaa ttacgtaatg accgacaatt cggactttga ttggtttggc ctgaatctcg
     9061 gagtggtgtt tgggttgtag aaccacaact cattcaaaaa accatcagca atcactgatg
     9121 gtttttttat gcacttgatt ttcttcttaa ttgaaggcgt gccgataaag cagtatttta
     9181 aattcactca tcctcgcctg caggaagctc cggcacttgc gtatcctctg ttcctggtaa
     9241 cacctgcaca tcgcgcagct ggcgctgaat ggcacgggtg cgggtcccct tgagctgatc
     9301 gatggtctct ccagcgctgg tgatctgctt ttgcgctttt tcgagcaagc cgccaaactt
     9361 ttcaaattca gttttcactg cacccagtat tttccacact tcactgcttc gcttttgtat
     9421 tgccaatgtg cggaatccca tctgtaaact atttaatata gcagccaatg ttgttgggcc
     9481 tgttacaata accttgaatt cacgctgtaa ttgttcaagc agattggaac gccggattac
     9541 ttcggcatag attccttcaa acggcagaaa cataatggca aagtctgttg tatgcggcgg
     9601 atcgatgtat ttatcacgaa tatccttcgc catttttttt atagtgtttt ccaaatcttt
     9661 gttggcagca tcaataacat ttgtgtccgc cgtttcgaat gcatcaacaa ctttctcata
     9721 tacgtcgcgc gggaacttgg catcgatggg caaatacaca actgaattca tatcatcctt
     9781 tcccggtagc tttatggcaa actcaaccaa atcgtttgaa cccagcttgg tctttacatt
     9841 tgaatcaaat tgttctgggg ccaaaatctg ctccaacaac atttcgagct gaatctctcc
     9901 cactccacca cggtccttca cattgctgag cacttttttc aaaccaccta catcctgtgc
     9961 cagcgactgc atttcaccaa gacctttctg cacttcgagc agctgtttgc cgactgtttc
    10021 gaagctttgt ccaagccgct cgctgagcgt tttttcaagt ttttcttcta ccgttaaacg
    10081 aatcgattcc agtttctttt cggtgtttac cagcaactca ttctgccggt tttcaagctg
    10141 cgaaaatttt tcgcgctgta cttcattaaa cgattgcaca ctcttatcga acgattcctg
    10201 aaaacctttt attgatttct ccagcacttc acgttgctga tcagcgtttt tcaccaactg
    10261 ttctgtcagc agcaacaact tttgctcaac agtggtggtg agtttttcct gaaccttgcg
    10321 attttcttcg gtaatcaacc gcagcgcttc actcatttcg gttttaaaat ccttcagcga
    10381 attgctgagt tcatcgcggt tttctttggt aagttttgca ctctcttcgc gattgcgctg
    10441 aaaatcatcc ttcaatccgt tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnattatt ttaccattct aaaaaaactc cctggcaccg gcgattcaag ctgcatcact
    10621 ttgccgctca ccggatgaac aaatgccagc aaagtagcat gcagacccaa tcttccgatg
    10681 ggattctgac cgtttccgta gcgacgatcg cccacaatcg gaaaatcgat atcgtataaa
    10741 tgcacgcgta tctgatttcg acggcccgtt tcgggcaata ctttcaacaa actaaaattc
    10801 tcattctgct gcaccaattc ccaatttgta acagcatgtt gaccgtcatc gggacgcaga
    10861 cttgaaaaaa cctgcagatt ggcattctct ttcagccagc tttcgatgcg gccccggggc
    10921 tcttcaatct ttccacaaac cagcgccgca tattcacgtt tgtgattttc gttgtgccag
    10981 tattcctgca aaacatcccg caccatggta ttccgtgcaa acacaagcac ccccgaagta
    11041 tcgcggtcga gccggtgtac aataaaaatc ttattgtttt tattttcagt tttcacataa
    11101 tggctcagca aactgtatgc ggtattggtt ttttcttttt cagtacccac agtcaacagt
    11161 cccgaaggtt tatcaatggc aataatacta tcgtcttcgt agagaatttt aattcctgga
    11221 atagctgtag ccggctttga gacaggactt ccgataacga ccaacatccc ggcattgaga
    11281 ataaaatcat gtcgtgttac cgtctttcca tcaatggtta cacgtccgcc ttgcagcatt
    11341 tgtttaagtc ctgtgcgggg ttttccggga aacacttttg tcagaaacgg caacaaggta
    11401 tccgcatttt ttaccgtaaa ctgtaatggc tttgacgttg atttcataag atttttcaga
    11461 ctgtaaactt acaaaataaa tcgccatgcc aatacccgta caaaccagaa catgtataat
    11521 caaaaaaaat ccccggagaa aaaatcaacg gggattcaaa aaaaggcaaa tgcgttttag
    11581 aataccacag tcttcaccat ttgcattccg ttcacttcaa gacgaatgaa ataagttcct
    11641 tttgccttca cctgattcaa ggaaagtggg aaacggtggg caccagcatc gagatttgaa
    11701 gtagttataa ctgtaatttc gcgtcctgta atatcaagca cagacagctt tacgttggca
    11761 ttgtcatgca atgtaaaaga aatatttgtt tcatctccgg cgggatttgg atatacagca
    11821 aagctattgt caacaatgct ttcattgact ccaaaccagc tgttaggatg aatattgata
    11881 tcatcaataa aaacattatt gccaccgttt gaataaaatt caaatttgac tctcaggttt
    11941 gcatagccag acaaagttcc ctgcggcaaa ataatttcgt gagtgcccca ctcagcttcg
    12001 gatgcgggat taaaagcagt ttcggcagga gaagcagaag caagggcgtt tccggtcaat
    12061 gataatttat tcacccaggt ttcgccacag tcatttgaaa aataaacagt aagtttatca
    12121 tacaccgtat cggctgcagc aagaagagaa ccagcaacaa attttggagc ataagcgcaa
    12181 cgaaactgca gtttggtatg ttcacccaat cccgacaaat cataagccgg tgtaataaac
    12241 gcatcgtaac taccggcaac attcccatca aaattattga ggtaaatagc ttttgctcct
    12301 gtgcaggcac tggccgactg agcccaggca gcgccggtat cattatggat tgtaaggtcg
    12361 ctgttgaaat tgatggtctc gaaagattca atcaaaggag caaccgtaac tacagaagga
    12421 tccattacat gaatatagga aacacgagtt aaagaatcac tgccagtggc attgtaaact
    12481 ttgagtgtta cagcatacac acccggtgca gcataagtaa caacaggcgt ctgatcggtt
    12541 gaggtagaag gtgaaccacc agcaaaagac caggtgcggc ttgcaacagc accgttgtat
    12601 gaaacatcgt ggaatgtgac agtatttccc gcgcaaatgg tatatgcctc tgcatggaaa
    12661 tcagcaactg gagcgcaaag gacagcattt gtatcgaaca caccggttgc aacaaggttt
    12721 tcgtaagacc aaagatttct acggtcattc atcagtgtag tattgatgcg gtcgagctga
    12781 cccaacgaaa acatattggt acaataaccc cactgataat ccatgtagtt ttcaatcata
    12841 tccggttcat cggggctatc ggtggtacag gaattaatgc tgccgggaac aaaacaggat
    12901 aaggaggtgt tttgcgtagc agcagcaacg ggcggcgtat catctacttc atcacctgtt
    12961 tcgaaaagag gaggcattcc gcatccatca atacccaatg cagcatacat ggattgaaaa
    13021 ggatggtaca aattgaaata atgaccggtt tcatgcgtaa aagtcctgtt catcaaattg
    13081 agacccatgc cagccatatc agctgcagta ccgattgttc ctatacaatc ctgacgaata
    13141 agcaccccgt ccattaaagt gtcgccacca gtgagcgcct gtgtaagcgt gttgctgggc
    13201 gggaaaggcg acatacctcc gatgaaagca ccatccggaa gactcatccc ttcgggataa
    13261 ataaaattga cggcaaaaat attcatgtat ttgcttggtg tccagcaata ttttgccatg
    13321 gtattgaaat aggcaaaatt ggtttgcgga tcgaaatgat gaataatccc ggaagtacaa
    13381 tttccgttag tgtcaatacg ggccagacgg aattcaatct gacaattggc tgcacgcgac
    13441 ttgaacagcg ggaaaatatc ggctgtatca gcgttttgca ggttgtaatc ggtattcagt
    13501 ttttccagtg catcatacac ctgatcatct gaaatatttt cagcaccata ggtgtgaatg
    13561 atgtgaaaaa cgaccggaat gatgcgtttg ccgccaatca gcgtgtcggt ttttgcactg
    13621 ttcatgagtg tttcaaaatc cgcctcataa gccacatagc ggtcaatcag ttccggatca
    13681 gtagctacag catttttcag caaaacatca tcggctggat acaatacacc actttgagcg
    13741 aaggttccga acgccataaa aatccaaaaa gaaaaagtca ataatttttt catatcctcc
    13801 aaattttttc ggcaaatata aaaaaatcta cgaaaaaata agttttgccg ccgatataag
    13861 caataccgca aacaaaacca cacgaatgaa accggcgccc cttttcaaag ctgtttttgc
    13921 accaaaaaca gctccgataa tactacccgc cgccaatacc agaccgggta tccaatcaac
    13981 ctgattgtta taaataaaaa cagccagcga aaacggggta aaaacaagag ttaatgccag
    14041 ttttaaggaa tttgcattga acaggtcgta accaatggaa agcaccagcg caaacaacat
    14101 gaaaatacca acgcctgcct gaacaaatcc accataaaaa ccgatggcaa caaacaaaag
    14161 acaatgatac catttgattg tgaaagaaac attctgagcc ggtttacgag aaaaagcgcg
    14221 gggcttccaa atagtcagaa cagcaaaaac aatcattaca tagccaataa atttttcgac
    14281 aaactcatca gggatttcaa ccacaaaaaa agaacctgca atggctccgg gaactgtaaa
    14341 agcagccaat atcaaagcgt tgcgtacgtc aaaatatttt ttcctgctga atgaaagcac
    14401 gccggtgagt gagccgagca aaatagatat ccggttggtc ccattggcga cattggcggg
    14461 catgccagta aaaatcaaaa caggaagaga cagaaacgaa ccactaccag caatggtgtt
    14521 gacaaacccc acaacaagac ctgttgcaaa caacaacgga taataccacc agagcataaa
    14581 acaattttat aaatgcaaaa gtactttgat ttatcaaata cgtaatacaa aatgcgaaat
    14641 ctgaaatatc aacgaatgtg atattcactt gaaacaactt gtcattactt gccggaaagc
    14701 taaaacaata aacaaaacaa ttaaaacaga gaggaaataa tggctacaaa tctcttttat
    14761 cagaagccgc catcatctac atcattgccg ccattaccgt tggcattgct gcgtttttct
    14821 ttcgatttat aatcgccgga gagtttccag gtgaaaccaa gcataaaaat acggccttcc
    14881 attttgcgct gcatatacat ttcataatct gcttccataa aatccatccg gaatttcatg
    14941 gtgttgaaca aatcacgtac attcagcgaa atggccagtt tgtcgtttaa aaaacttttc
    15001 ttcactgcac agtctgccat aatcatggca tcacggatgc cctgtccggt ttcgcgcggc
    15061 gatctgtaga aaacagaaaa acttccttcc cagcccttgg caaaacggaa cgaattgctg
    15121 tttcgcaatt gccatccaaa agctgcagcg gtaatactcg tttcaacatt acttccatca
    15181 acaatatgtc gataccacga accactgatt gttgtattcc accatttcag gggcttatgt
    15241 gtataaatca tttcaaaacc atacgaagta gccgaagtat aattttcggg catcattaaa
    15301 tacaccgaat cattaagatt catgcgataa cgagaaataa catctgtgat gcggcggtaa
    15361 aaaaccgttg gcataaaagt tcctcgtttt gaataccatg cataactcat ttcaagggca
    15421 ttcacatatt ccggatcaag aaaaggatta cccgtacgaa tattcggcca cgcactgtaa
    15481 tcgcggtatg gattcaaatc cctgaaactc ggacgattga tacgccggct gtaggtcagc
    15541 tgaatttcct gcactttgga aatcttatag ctcaatgccc cggtaggaaa caagctgtaa
    15601 taagtctttc tgttatcaag ggaatctgct tcacgtgcat ttgtagaagc tgattctatg
    15661 cggcttccga tcgatagtcc ccacttcttg atttttgttg aatagtttgc atacaatgct
    15721 gcaatatttt cattgaataa aaaatgattg gatgtagtat cgttgaaaac ccaacctgat
    15781 ccgccaaaat cataattata ataacggctg tcagcatcga ttgagcgcat agatccatca
    15841 aatcccgcct cgagttttgt gctgtcattg aaaggataaa cataatcgag tttcagcgtt
    15901 ttcacactgt tcaggtttgg tgcaacagtc tttgaccatt caggtatttc ggatgtaacc
    15961 gttgcaaaat cttcttcgag aaatgtaatc cggttaaaag aactgtcctt ccgattgcct
    16021 tcactcattg aaaagtcaag tataatttca tgtttgggct ttgtaaaacg ctttttaatg
    16081 ttcaaattgg catcccaatt attcatagaa gcattttcga gcgaattgtt gctgtaaatc
    16141 tgggtgtaaa tattttcggg attcattgta taattcatgg ttttctgatc ttcccgatca
    16201 ctgaaaataa aagtagatgc cgatgctgca acaataaatc tgggtgtaaa atgatactcg
    16261 ccgccaaatt ttatattgtg cgactgattg ccatgaatct catcaatatc ctgacgcaga
    16321 aaataacttt caatcaaatc ataagtgatg cgttcacttt ctcccgtcac agttcgcttc
    16381 ctgttctgat aattataatt gccgaacaat gtccatttat cagtagaata actggccgaa
    16441 aaagatgcgc tgtatttatc ttttgtgccg gcattcaacg ataataacgt attcaatccg
    16501 gttccttttt tctttttaag aataatgtta atgataccgg tcattccctc gggactgtat
    16561 ttggcagaag gatttgtaat taactcaacc gattcgatgc tgctggccgg aatctgttcc
    16621 atggctgcac ggcgatctgc acctgtcatg gtgcttggcc ggccatcaat cagaattgta
    16681 acattttcgc tcccacgtaa actcacattg ccatcctcat caacttcaac actgggcacc
    16741 tccttcaaaa catcgagtgc ggtaccaccg gaagtgttga ggttttgagc cacattaaca
    16801 acctttttgt cgatactaag ctcaatcatt ggtttttcag ccacaatatc tacgcccgac
    16861 aaagactgag ctgatggggc aataaggatt ttcccaagat cgaaaagggg tccagagcca
    16921 ggcttgagct gaaccgggcc aacactttta ttggcatatc ccataaaatt gatgcggagc
    16981 ataaacctgc ccggggtctt tgacacaaaa ctgaattccc ccatggcatt ggtaatacca
    17041 ccaccagcta tactgctgtc gcgcatagaa acaagggcta ccgttgcata ttcaacgggt
    17101 tttccactga cagaatcaag aacaattcct gtaatctgaa aatttgcatc aggcatattg
    17161 cctggctgag caaaaccaaa tgaaaaaacc atcagagata gtacaaccat taaaaatgaa
    17221 aaacgaagca taaaaacaaa attttcttat tagacattga tttcggctgt ttcttgcgcc
    17281 aaaatgcaaa aatacctaaa atattacgaa aacaggccca aacctgattt ccatcatatt
    17341 aatgccaatg agacaaaaaa caatacagac caaaaaaaat attgtttatt gtacaaaatc
    17401 aaaaattagt ttgtatattg cattcaaatt caatgattat gattaaaaat atatcttttt
    17461 ttgccgtcat catcatttta gcaatgacgt catgtaaaga cgataaaaca gaatacaccc
    17521 catttgaacc ggtaaataat caggccgcac acaccagtgc ctattacaac gcaggtctgg
    17581 gcgacaatcc cggcgttcca tccgggacac tgtattcatt cccttcaggc atcgaaatta
    17641 ttggatatat tcacggaaac gcacccacag tgcttagtgc ccctgttttt gacaaaatca
    17701 aaaatccagg aagcatgccc gattattctg atccaaaact caattatctg agtctgggtt
    17761 acggatctct tgtcaacctc tatttttcag tgtacaacaa cacaaataca caaaaatcag
    17821 tctcgattcc caaaggaacc atttgttacg aagccaatca cacacccgca gaccagcatg
    17881 gcattctcgt gaaatcagtc agtttcaacg ttccggcaca cgataccact gatgtgcatc
    17941 tggccttgtt ctgcgtcaat gcccatcatg gaattgccac agccaccgat ccatataaaa
    18001 ttggtgtaat taccattcat cctgatttaa ttactgtttg caatattctg gatgcaaaac
    18061 ccaccattga tccaaacgac tatggcacta tccagtccat cctctggaaa atcaccgaca
    18121 atagcggatt tggacaagcc gacattgata cactcaatgc tatgtaagtt aaaagcttac
    18181 cttggttcat taaatcatta tgattcatca tcatggatga attattgata atttggcttt
    18241 aaatgggcca ttttataaaa atcagcagtt acaaatcaga aaagacacat tcgtctttca
    18301 aaaatagctg gatccgttga agcaatgagt acaaatttta tctttgggta atccaatggc
    18361 ttcaatcagg tcttctaacc gttgaaactt cagcgaatcg agtcccagcc gaagccggat
    18421 gcgttccacc atttgtttgt attcttctga atccggattg gagtacaccg ccatatcgcg
    18481 ttcttcctta ccttccagtt cgtgcatggc gatatatgtt gccagatcca tattggaacg
    18541 tgaacggctg aagttaagaa aaggacatgg ataggttaac ggcgggcacg caatgcgcat
    18601 atgcacttcg cgaatgccgg cttttcgcag atccttcaca ttatctttaa gctgagtacc
    18661 acgtacaata gaatcatcaa ggaaaacgcc tgactgattt ttaattacag ctgtattcgg
    18721 aatcaatttc atggttgcta ccaaatcgcg ctgcgcctga ctttggggca taaaactcct
    18781 tggccaggtt ggtgtatatt tcgaataagg acgtttataa gggatgtgcg taaaatttgc
    18841 ataacctgtg gcatgaccaa caccggagtc gggaatacca gccacaaaat cggcattcac
    18901 actgtcattt tttgccaagg cttcaccaca acggtggcgg cattcatcta cattgatacc
    18961 ttcgtatgaa gaagtcggga aaccataata cacccacaaa aaagcacaga cctgcatttg
    19021 gtcgttgggc tctttcaact ttgtataacc atcggcagtg attttcccga tttcacccgg
    19081 acctgcatag tgaaatgttt cgtagccaag atttgggaat gcgcaagttt cgaaagaagc
    19141 ggcaaaggca ccaccttttt tcccaatcac tataggcgtg cgcccaagtt tatcgcggcc
    19201 gatataaata caatccgaag tcattaccat cagtgagcat gaaccctgga tcagctcctg
    19261 tacattgcgg atacccgaaa caaagtcttt ttcttcagca atcaacttgg caacaagctc
    19321 cgttggattg atgtttccca tgctcatttc agaaaaacca tactgcttgt caatgcaacg
    19381 tttttcaagt tctttaatat tcacaatttt ggaaactgtt gctacagcaa atcttccaag
    19441 atgcgaagtc attacaaccg gttgagcttc ggtatcgctg ataaccccaa gtccgatatg
    19501 accattgaaa gcgggcaatt catcttcgaa tttattacgg aaataactat tttcaatact
    19561 gtgtattgcg cgctggaagc cagcttcctt tgagtaaaag gccaaccctg cccgtttggt
    19621 ccccaaatgt gaatgataat cggttccata aaaaacatca ttgacacaat cgcccaaaga
    19681 aacacttccg aaaaatccac tcattacaaa atgaagttaa aattaaagtg caaaggaaca
    19741 aaaagaatgt actcaaaatc ccattgttga taacaggttt ataaataaat gacgtcggct
    19801 gaaaaacacg caactcactc aaaaacaaga gtttttgaaa ttccgaaacc ggaaaaaatg
    19861 actatgggca ttttataaat cattataacg aaatacattc tgttttcaca aaaaatgcag
    19921 catttttcag atgctgcatt tcagaaaaaa atcttcaaaa tctatttcca gcggcgatac
    19981 tgttttatca gactcacaca atcgtcttcg ttgtattcat taactgttgt ggtaagttca
    20041 agaaccttgg ttttcggagt ccagaaaatc acattcataa aatctttatt tccataggtg
    20101 ccattggtcg attcaaaaga ttcgctatca atggtttgac caacattaaa gcagatcggg
    20161 cttttcttgt tcatctgata aagccgtaca gcttcgagtg aggcaaaatt cattgtaacc
    20221 tcaccgttga gttttccgtt ttcttcgtac agtcttttct gaatatcgct tgcaagagga
    20281 aaattatcag caaatttttc accattgaga tagtcattga ttaattcatc aaaatcctgc
    20341 tccgacacat cggtagagtc gtccatctgg ctaaagatgt ttacataagt aatggtcagt
    20401 ttacctgtgt ttttgccggt aatctcaaat ttatatttct ttttctcaac tgttaaacag
    20461 ctgcttatca gaaatgccaa aaaggcaacg gcgacaaatt ttaaaatttt cataggttag
    20521 ttgtttgttt tataagacaa aaatacgatt tcttttcaat gatgcagatg aaatattatt
    20581 ttttttattt tttatgcaga atttatcgtt tcagattatt cgcatgaacc atccgtattt
    20641 ctttttgttt aagaatttca ttgattgaag tccaaccagc cgtatgtaaa tgcagtttgt
    20701 ttatattggc aaccgtaata aaaaaatcct gatgaagttg cgccagatat tgctgttcag
    20761 tttgtccacg ggtgggaaaa atgatggccg aacggccaac tgcaaataaa tccatcatgg
    20821 tgctataccc tgcatgagaa ataataacag cagcttctga aagccattta gccaactgct
    20881 catcggaagg ggacattata caagatatgt tttcaccagg gtcattgatt tcggcaccag
    20941 ctataataac tgacaaccca cattcagcaa acacctgtga taattttcca gccatttgcc
    21001 tgcggtgttt caaaggaccc gaagtaatga ccaaaagcaa ttcatttctc actgttgagt
    21061 tgtaatcatc caaacgatca aaacgactta gcggacctat atattttgcg tttgacacta
    21121 aagtcttcgg gtgactcagt ttaccagcaa gtcctgggaa atttttataa tccggcaccc
    21181 agacttcgct aaagcgggcc aaagattttt taataaattt tcgtaaaacc ggtaatccaa
    21241 aacacagcca ccatggcgca atgggctcca gctgatgggt tatcaatacc gatctgatat
    21301 tatcgcatct gaatccatat cgattatccg acacaataag attatattca ttactggcac
    21361 acagagccga acacatattc tgttctgatt tctgctgttt gatcattttc ggaattaaac
    21421 cgataatctg aaaaaaacca gttccgttaa acccgtaact aacaggtttt gttttatctg
    21481 ttataaaaga aagatcggga aaacgttgct gcagatagct gcaaatgcca ggagaagcaa
    21541 aaactgttac agcttgacct tcagcaataa actgttgtat cagaggaata caacgagccg
    21601 catgacccag tccccagtcg agcggagaaa tcagaacacc ggtgtttttt ttcaaacaac
    21661 taaaccttta aagacagctg aatgataaat gtccggggtg gctgaacatc gccggggcgc
    21721 atcatgtatt ccctgttgaa aatatttttt gcggtgaaat tcacatgaat catatcattg
    21781 attctccatc caatccgcat atcaaaaaca agaaaccctt tatcattctt ttccctgtat
    21841 tctttcaatc caggcaaaat cattgcgtat tcaccagtag cagggttaaa caacggggta
    21901 gttccatcgg ggcccctcac cgtatcttca aatattttat cgatattgat gatatgactt
    21961 tgatactcga aattggctcc cagagtcagt ttcttatatt caaccgagaa gtcggctttg
    22021 gcgctgtgat aaaagcgata cttaagaata ttgtctttgg ttgatttcat agaatcggcg
    22081 gtagcatcga ggtcaatggg gttgttgtaa gtatatccca acaacaatgt ggcgggtaat
    22141 cctatgaatt ttccttcgcc ggttactacg atttcaaatc cggaaatttc ggcattacgg
    22201 acgttctctg ctttgaagcc aacccaattg agaaaataat tgggtgaagt aggcgggaat
    22261 ggaataagaa tgacactgtc gggattatga tagttgaaca taaattcaat catattttcg
    22321 tagcgggtat aaaagccagc cacatcggca taaccattcc aaccccacaa gcggaaacct
    22381 tgtttaaaag caatctctgt gctataaccg gtttcgggtt gcagattggg atttggaaaa
    22441 acctttaaat cgccggtgct cgtggctgta tatctttccg caatggtagg atagcgatag
    22501 cccataccaa aagatgcacg aaggaaagat tgttcagtga cagaataatt gactcccgac
    22561 cggaataccg gtttgaattt catatcatcc tcatcaatac gataaccttc gatgcgcgca
    22621 ccaagagata cactcagacg attgaacttt ttatccgcct gagtgaaaaa tccaatactg
    22681 gagccaaaat gacgcgtatc gccataaatc tgcgccacac tttcattata attggcacaa
    22741 agaccattgg tccagttaag cgattttgaa ggatatcctt tataaaaatt atactcaccg
    22801 taatacatat catcgaaatt atcctgaccg gtattattgg tgttatgcac ctgaaaaaaa
    22861 cgacctttca aactatgctt tgtactgtcg tttttatacc agataacgga tggatacaaa
    22921 ttgacgtagg aattattaac ttcctgctgg aatgattcat tgcatttata aacgccacta
    22981 tcaccatcgg cccataaaag aaaaatatcg ccgatcctgc gcatgtagtt ggccgataat
    23041 tcataggaaa gacctttaac ttttttatca cgataacgga tagaaccatt cagacgataa
    23101 cgttcttctt cgttgttggt gcgataacca ttattcgaag aaacaaatcc accaaaagtg
    23161 atatcaaaat tgcctgcctg gcggctatgc agaaaacggg ttgatccaaa aatgggagca
    23221 ttattacccc accatgcaat ttctttacgt tcaggtgttg tatattgtcc gaaaatactt
    23281 gtaatatagg ttttgggaac aacccccgga taagccgtgc ggtaattaat cacaccattg
    23341 agagccgaag aaccatacag tgccgagctg gcacctttaa tcacttcgac ctgctgcaca
    23401 ttttcaatgg gggcgaaatc ccagcgcgcc tcacccgacg cacctgtaat catgggcata
    23461 tcatcgacaa gaaaaagcac acggctacca gcaccataac tatagccact tccgccgcga
    23521 atcgatggct gcttgtccat aatattcaat ccggggattt tctgcaaacc ctcttcaagc
    23581 gagcttacat tggtattcct gataaaatca gctttaatga cttccatcga aacggtgaca
    23641 tcggaaagtt tctgttcata ttttccggca ctgacaacaa ctgcatcgag aattctggat
    23701 tcggttgtca ttttcatatc gagaacaaga gtctcacctt ctttcagatt aatcagtctg
    23761 atttcctcag tataaccaac aaatctgaat gcaatggaat gtgttccttt atcaagggtc
    23821 agagaataat ttccgtcaat atccgtagca acaccggtag ttttgttcac caccacattg
    23881 acccctggca gggtcaaacc tgtttctgaa tcggttactg ttcctttaag aatggtttgc
    23941 tgagaaaaac cataaaacgg aaaaatcaac attaataaaa caaatggagt aaaaaatttc
    24001 ataggccaat tgaattttta gactaacttt gctcacctat tgcatcaaca gccctttgta
    24061 agggagtttg aagaaccaaa agtaattaaa atttctaact gtaaattgaa taacaaactt
    24121 ttatatctaa ttgcactgaa atgcattccg caggtaggcg atattgtcgc ccgcagactg
    24181 atagaaacaa ccggcgatgc ctctgttgtt tttaatatga cagatgagaa aattgaaaaa
    24241 ttacccggtc tgactccaaa aaccatccga atgatccggg aaaacaggac caaggcattg
    24301 caaagagccg aaaatgaaat agaattcatt gataaacata aaattcaatg tcttacttcg
    24361 tttgacgaca catatccatc tcggttgaag cactgcctcg atgcaccttt gtttttattc
    24421 taccgaggag cttcggccgt agaatcggat tatatgactg ccgttgtcgg cacacgcaaa
    24481 tcaaccgtat atggacggaa aatgacagag caactcgtat caggtctcag tctgaaaggc
    24541 atgactattg taagcgggct ggcctatgga attgatacag tagcccatca aactgctgtc
    24601 gattgcaata tcccaaccat cggcatcatg gctcatggtt tcaaacattt atatcccgaa
    24661 gaaaacagga aactggccac actaatgctg aaaaatggcg gattaatgac agaattcctg
    24721 tcggatgaat tacctgaaaa acaaaacttt cccatacgca accgcattat tgccggcatg
    24781 gccgatgctg ttgtggttgt cgaagctcaa agcaaaggcg gcgcattaat taccgcagag
    24841 attgccaact catactcgcg cgatgtattt actattcccg gtcgggtgga tgaccagaaa
    24901 tcgcgcggtt gcaactggct catacgcacc cagcgggcaa cactgattga atcggccgaa
    24961 cacctacggt attttatgaa ctggacacaa gccgaaaagc ccaaaataag gcaggttcga
    25021 atggaagtat tgctggatgc cggagcacaa tccgtttatg attatctttt tgagcatgga
    25081 gaaaaaccca tcgactccat ttgcaatgat ctgaatatca gcatcaatac agctgccatg
    25141 atattgcttg aacttgaatt tcaaggagtt gtagcagcat tgccaggcaa aagataccgc
    25201 ctgaaaaatt attgaacttc ttttgttgat aaatattagg aacagccctg caaaatactg
    25261 tcctaatatt cgtatattta tcattttaaa actaaaacaa acaccatgaa aaaattatta
    25321 ctttcttcaa gtttgattat tgcattcgca atttcactaa gtgcacaggt gaatttcaca
    25381 cagaattttg acacctgcac agcgggagat tatgctgcag aatgtatcgg agcgccatgg
    25441 accacatgga gcggcacggt tggcacagcc gaagatgctg ttgtttctgc aacacaatct
    25501 tcaagcgctc ccaatgcggc ctatatcgga tcaacctcaa ccgactttgt tgttctgctc
    25561 ggcgatctga caacaggcgt ttacgacatt agttttgatt tctttgttga aaacggcaaa
    25621 atggggtatt tcaacatcat gaacagcttt acgggtggca catatttgtg ggcaaccgat
    25681 gtgtatgtac gctcgacagg atatctgtca tggactgcag ataatgtttc agactccatt
    25741 ccgtttgctt ttgacacatg gacaaaaatt ggctatacca ttgacctcaa caacgatact
    25801 gcctttatgt atgttgatga ctctttggtc aacagctgga aattcagcaa aggaacactg
    25861 ggaactggta ccacgctgaa actggcagct atggatttct atggcgcagc cgaagatctg
    25921 cagcccggtt actacattga tgatttttca tttgcaataa acacaacagg tattgttgaa
    25981 aatgaaaaca atgtatcgct ctatccaaat ccagctacca atgccattac catcgaagga
    26041 caagccaaca accgcttcga gattttcaat acaggcggtc agatgctact gtcaggaata
    26101 ctgaataata attctgaaac cattgacata agcactctga acgcaggttg ttattttata
    26161 cgctgcatcg gaaatgatgt aactgtaaat caatttattg ttgaataaag acttttcaaa
    26221 ttaaagcaaa gccactcttc ggtgtggctt ttttgttttt attgaccgtg aataaaaaat
    26281 atattataat tttatcgctc aaaaacccac aaaccatgaa acaaattttt ctatcctttg
    26341 cattatttat tatctgtgct gttactttgc aggcacaggt aatcatcagt cagaactttg
    26401 atgctctgac agccggtgcc ggaacctacg cagcacaaca actcggcgct ccatggacga
    26461 cctggagtgg cacaaccgga aatgccgaag accctgctgt aatgaccact caggccgcca
    26521 gcgctccaaa ttcggtgtat attgccacta ccagcaacga ctttgtatgt catttgagtg
    26581 acaaaactac tggccgttac aaactgaatt ttcagatttt tgttgaagcc ggaaaaatcg
    26641 gatacttcaa cattctgaat gattttgctg gaagcagcag tatctgggct atgcaggcct
    26701 atttcaaatc aaatggttat tgtgttgttg atgccggtgc tgcttcggca gactcgctgc
    26761 cctacaccac caatgcatgg catgaagttc tttttattat tgatgttgat gatgactttg
    26821 ccaccatgta cctgaacgga actgaactgg tcagctgggt tttcagcggc ggctcatttg
    26881 gtgacggcac aacccacaaa ctggatgcag tgaatttcta cggttggtcc gacgatctgc
    26941 aacccggtta ttacatcgat gatttcaatt ttgagcaggt aactgttccc gaagccccga
    27001 tgaatctgac tgcagctgtc accggttcgg atgtagctct ggcatggaca gctccaacca
    27061 gttcaccaga ctcatacact attttaagga ataacactgt tcttgcttca ggaatcacaa
    27121 cactgaccta tgctgacaac aatctctatc cacaggatta taattatgtt gcaaaagctc
    27181 attacaacaa ccttggttat tctcattcat ccaacgacac taccattacc attgccggtg
    27241 gtattacccg caacaatgtt ctctttgaag caacaaccag cacctattgc acatactgtc
    27301 cgggcgttgc acttgcaatg gaagaatttg ttgccaatgg aaaaaatgtt gtaattgtcg
    27361 aatatcacaa cgattggcag ggtccagaca cctatgtaag cacagcaagt caggcacgtg
    27421 cagttgcata tggcagcgca ggaattgcta ccggcaaccc gacaaccatt gccgacggag
    27481 acctctatct ggtaggagga aatcacacca tcagtcttta tccgaatttg ctgcccatgt
    27541 acgatgcccg tattgcgcgt aatgccattc atgatattga cctctctgtt gtaaaaaccg
    27601 gtgttgatac ctacgaggcc actattattg ttgaacagat cagctcgtat tatccaggca
    27661 atctttattt acgcactgct cttacggaat ccaacatcgc cttcccatgg cagggtcaga
    27721 ctgaacttca ctgggtttgc cgcgacatgt atccgaatgc taccggaaca gcccttgatt
    27781 tctcttcagt atcaacacag acttacacaa caacattctc aacaactggt tacgttgccg
    27841 acaactgcga atttgctgca tgggtgcagc ttggcgccac cggggatgtt acacaggcca
    27901 taaaagttga cctctcaaca gttatcggaa ttgaagaaac gaataactct ttatggagca
    27961 tttatcccaa tcccgctaat gaccagatta ctattgcagg catcgaaaaa gctcaatacg
    28021 aaatcgtaaa catccagggc caggttgttt tatcaggtac cattgaaaac aatactgaaa
    28081 ccatcaacac cagcaacctg aatcagggta gctacttcgt ccgcatcgtt ggcgaagatg
    28141 ttatggtaaa gtcatttgtg attgaataaa acatttctcc tctttgataa aaagccatcc
    28201 cccggggtgg cttttttaat taatccatct cacccgaaag cagcacgtct actttgtttt
    28261 aatcgtacta cctgatttgc aaaaattcag ctgcaatatt ttgtatcttt gtaaaacaaa
    28321 agaatagcaa tgacaaccgt tgaattaaag aaactcctga ttcaaagaat tgctgagata
    28381 aatgatgttt catttctcaa ggccgtaaaa acaattcttg aatcaaaaac aaacacagaa
    28441 attcttttac tcacacctga gcaaaaaaaa gaaatcttgg agtcgaaaaa aaacattgaa
    28501 caagggctta ttattgataa tgatagtctt gacaaagaag ttgcaaaatg ggcaaacgca
    28561 agataatctg gtcagacaga gcaaagaaaa aactctttga aatattggag ttttacgaac
    28621 aaagaaacca aaataaaaca tactctatca aactatacca aagatttaaa aaagaattat
    28681 ctgttcttcc caaatatcct gatattggca ttaagacgga aattgaatcg gtgcggggct
    28741 taattgtaga cgatttcata cttttctacg aacctgacaa agaaaagatc attgtacata
    28801 caatttggga ctgccggcaa aatcccgatg atttgaaaat tcaacagtaa taattaaatt
    28861 cacacttgtc ctaaaaatca aaaaagtcaa tcaccaaatt catcaacaaa aactcaacaa
    28921 aatggcacta ctgattctgt taattgttgc atggatcgtg attattggtt atgaagcaaa
    28981 aaatactggg gtcaatgcat ttctttggat tgcaacagcg attatttcat atgcggcttt
    29041 ttcagtaact gcattctttg tttttataag cttttcacct tgtaatccgg aagaatgtaa
    29101 accagtgttg tatgggatgc ttgccggcaa cataatgcct ttatctatag ctgtatattt
    29161 atttttcaaa aaacccaaca acaaataaaa cctttcctgg atagtagtgg gagtaaataa
    29221 caattaaaga aactaataat ttgtcaggtt tcaatttaca tcaccccacc tccatctcac
    29281 ccgaaagcag cacgaattct ttgtttcggt cgtataccac ggcgaactgg cc
//
LOCUS       DAOPKE010000032        27615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1835_length_27615_cov_14.490348, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000032 DAOPKE010000000
VERSION     DAOPKE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_27615_cov_14.490348"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1326
                     /locus_tag="PKY63_05910"
     CDS             91..1326
                     /locus_tag="PKY63_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPF00182.1"
                     /translation="MTTGIIITLCVLILISYVFDLTSSKTRIPSVILLLLIGWLLQLF
                     AVQFGINVPDLSEALPVVGTIGLILIVLEGSMDLELDKSKKRVVLNSVFMALIPMLIL
                     GTGLAFIVQEVADVSFRTALINVIPLCVISSAIAIPSAQSLCKSNRELVTYESSLSDI
                     FGVVLFNFIALNESFGWNTFGNFFLQILITLVISFAGIIGLSVLLHSITHHVKFVPII
                     MLLILLYAITKVLHLPGLIFIMLFGIFLANLQLLHKVKWVKVFHVESLKKEYLRFKEI
                     VGEGSFLIRTLFFLLFGFMIKTEELLNIQSLPWALAITAGIFIIRFAFLKLLRIKEIT
                     FLYIAPRGLITILLFLSIAESDKIALINTSLVIQIVISTALIMMFGLMTNKKVKSPDI
                     PAESASEPIHEEPQNGAIQ"
     gene            1406..2707
                     /locus_tag="PKY63_05915"
     CDS             1406..2707
                     /locus_tag="PKY63_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HPF00183.1"
                     /translation="MSKTVLVAMIVSGIILTGCNADEQLGLDIQPPGDELNAVKSDTF
                     SITAYTRQADSVRTDETAISLAGYYTDPVFGSLRASFCSQLRLSGTDVNFGNNVVIDS
                     VILHLEYYNIYGNNKENNRMTFNVYELANAIYVDSTYYSFSPIASGRKLGSISFLPNN
                     EDSIYINGKRLGPQLRIPLDKNLGKRIVEASSMGYLASNVLFEDFFHGISVVPTAENP
                     NGSIISFDVLSANSGLTVYYHNDSDTLSYKFNISTTSARYSSFNHFSFNGAHPDLLGQ
                     VNGDTALGQQKLYLQPLGGTEILLNFPDIQSLSDNVFSVVINRAELFIPADLSDISNE
                     TYPRPVKLTLVELDENGDYAYLSDYTINSETYGGSFDENLGCYRFVITRHMQDIITGG
                     ETSYGYVLMVSGTSIYGNRVILNGPESTNPMRLELTYTLIE"
     gene            2726..4567
                     /gene="glmS"
                     /locus_tag="PKY63_05920"
     CDS             2726..4567
                     /gene="glmS"
                     /locus_tag="PKY63_05920"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPF00184.1"
                     /translation="MCGIVGYLGPRQAYPILINGLHRLEYRGYDSAGVALLNGDLRVY
                     KSMGKVRDLEELVKDKNLSGTIGIAHTRWATHGEPNHVNAHPHISPSGKMAMIHNGII
                     ENYAILKEELINRGYKFKSSTDTEVLIHLIEDIQINEKTSLVEAVQIALSQVIGAYAI
                     VITSQDDPDLLIAAKKGSPMVIGIGSDEFFIASDATPIVEYTKNVVYLEDEEIAILER
                     GQELKIKTIKNIDKTPYVQKLELNLGAIEKGGYPHFMLKEIHEQPNSIRDSMRGRLSA
                     DQGIISLGGIVDYEQKLCNAKRIIIVACGTSWHAGLVGEYLIEDLARINVEVEYASEF
                     RYRNPVITEDDIIIAISQSGETADTLAAIKLAKSKGATILGICNVVGSTIARATDAGS
                     YTHAGPEIGVASTKAFTAQVTILTMLALMLGHLRGTISKSKYNQYIHELDRIPEKAKI
                     TLEKAHGIIEKIAEQYKDVRNFLYLGRGYNFPVAMEGALKLKEISYIHAEGYPAAEMK
                     HGPIALIDEKMPVVVIATNKGTYDKVVSNIQEVKARAGKIISVVTEGDTAVKEMSDYV
                     MEIPETEEFLVPLLATIPLQLLAYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            5930..6373
                     /locus_tag="PKY63_05925"
     CDS             5930..6373
                     /locus_tag="PKY63_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00185.1"
                     /translation="MQPKILTILILFLSLNVFGQNNSTQDTKTINGFIVPVDLLDPAY
                     PIMKNTGDPVKDAEDFRLALRIYSKNLDRFPVYKNSGDEAKDEAAYDEACKTFFARHP
                     YFPQPINTNNPRLDEQNFDRWYKAYLKFYPEKAKRVAVIEEGGVK"
     gene            6370..>6563
                     /locus_tag="PKY63_05930"
     CDS             6370..>6563
                     /locus_tag="PKY63_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00186.1"
                     /translation="MRSILTFVFSVFSLYLFSQTTTFTTSGTWTCPAGVTSVTVECWG
                     GGGGGGGTKNDYKYGGGAGAG"
     assembly_gap    6564..6663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6664..9847
                     /locus_tag="PKY63_05935"
     CDS             <6664..9847
                     /locus_tag="PKY63_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00187.1"
                     /translation="GAGSTGAGGNASGTTAGSGTTIDGGNGASGSTTESDGSSGFTYG
                     GGGSGAFVPDNTNHTGGAGAKGLVKITPVYAAPANDLCANAVSLPCATTNLAGTTVGC
                     VAETSPGGCGSQYGVWYKFTGDGQQTTISCTATSSWDHEIDIFSGTCGTLTTVSCVDG
                     ALSAGTESYTFTTVNGTTYYVYVCQYSTSSTVTGPFTISRTCTAAGAACIPNTVIASL
                     PYSQTGMTTNGAGDDFSSSDACGSSYMNGDDYTFSYTPAANQCISVTLTNTDTWVGVF
                     VTQGCPQGGTCVASTTSSTGNPSLSFVSLTGGVTYFITISTYPSPQFTPFDISIQSIT
                     CPTPPANDDCSGAVSLTSNQYSCTSFASGTVVGATGSSQGNTCSSTYDDDDVWFSFVA
                     SSTAHQILISNITGSYTSMYFAVYSGTCPTLTEVDCFYDESGTVTGLTIGHTYYVRVY
                     TATATPLQTTTFDICVTKLCNAPTSIITSVSPVGPITSATSVTFDVDSHTGGACDGSW
                     EYQWETTSGTVLQAWSTTSAYTATISSTQNLRVMMRCSSCPTSTNSGTASIMFVNPMT
                     NNECSTAVSLPVGTSCNYETYTLDDGPHAATASSNDQPTCTAYDASPDVWFSFTVPAS
                     GNYVVDVSGGTGMDEVFCLYSGTDCNSLTFLTSPGCVDYPPTGTLSGTPGETIWVRVW
                     EYYGDNFGNFDICVKEITAATFTSGQDCELGYTVCSDQEFTGNSSGGGVEELDATNQG
                     CMSTEHESSWYLFSPQSAGTIELAITPDVWADDYDFAIWGPYPEGSMASDVCPPTGEP
                     LRCSWSAADGTTGLIDSAAVTDDTEDAGGDKWVNEIVVTPSDVNSVYVLLLDNFTTSS
                     EPFTLNWTLTDVILDCTVLPVILLNAYAECSTDGVLLSWTTVSETNNSQFIILRDDGS
                     GDYIEIGRVSGAGNSNMKQFYTFSDHFKGEGTVYYRLDQIDFDGKQTTLKTMTVDCYH
                     LSGFNINSASLDANAGNLEINFEGFANQEYQLNLMDLSGKVIFSDSFIAANDGKNQAV
                     SYFKPVAQGVYVVSLTNKSGITKFSKVSPR"
     gene            complement(9905..10771)
                     /locus_tag="PKY63_05940"
     CDS             complement(9905..10771)
                     /locus_tag="PKY63_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00188.1"
                     /translation="MKSISVFFILICSVIGSVVSQNQSGVQYTPLGTILQNSSFEFSA
                     RYIGSMEAMFIEAAMKNNTNQPMNVLVEPGRRFIADTARCQDLLNVKSYYVTIAPNSE
                     TKIKIRGFCCESSNSSPIYDMGYDVGFVAPEPWVKLASFIDRNSFPDGAVQHAVWGIS
                     NGHDIAGVDCGSREQTIALRKILCDLTDKEMPWTTVQYKQDSTVVFTGAVTRIRGDIH
                     YYVRYNCPITIILTDNNGRLIKTLMEPAIHGIGRYTFTLDLDVSDYPSGDYEVRIYED
                     GAILLDRRKFGI"
     gene            complement(11092..12156)
                     /locus_tag="PKY63_05945"
     CDS             complement(11092..12156)
                     /locus_tag="PKY63_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00189.1"
                     /translation="MNGTKFFASAFRLLLVIGLALSMMMMGSCKCDRDKKEFDREVSG
                     SKLDSLSFIKVSINRYEKDLFAIPVDSLKEGLTSMQKKYSFFYSPEDLNDPVNIAYLQ
                     QYLKDPTILSLYRETMKQYPSLTWLESELTMTFRRIKYLLPLWKVPTVYTYVSGGDIE
                     YPVKYADDNLVIALDLFLGEKYPIYKMWGVPQYITNRMAKDFLVVKCASEICRAWLEK
                     HSPAGKTMLDKMIFEGKLLYFTDVTVPDCADSVRIGYSGQQLYWAEKNQGNVWGFFIE
                     KKLLHTTEMRDINKFIGEAPFTSAFSKTSAPRIGRYIGWQIVRAYMAKNKDITFEQLL
                     NNNNSQEILNKSGYKPEKAE"
     gene            12200..12880
                     /locus_tag="PKY63_05950"
     CDS             12200..12880
                     /locus_tag="PKY63_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF00190.1"
                     /translation="MKKIVLALILFAAFAFTSNAQNEKFFRFGLHANTGLTWMKPDQD
                     SLEYQGMKIGLGWGFIGELTIADNFSFATGFDINYMGGKLQKIGVNQIYPPDTAAVFS
                     KLTSNYRFQFLDIPLTLKMKTNEINYITYFARIGGSIGICLNAKADEEYSPLSGSQML
                     SLNDQNLKEERNFFRANFLISGGLEYSLGGTTSALAELSFVNGLTYQLEDNKSIGNYI
                     MLKFGIMF"
     gene            12946..14571
                     /locus_tag="PKY63_05955"
     CDS             12946..14571
                     /locus_tag="PKY63_05955"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPF00191.1"
                     /translation="MKIALCQINTHTGHFAFNRKKILEYIERSKLENADLAVFPELCI
                     SGYPARDFLDFDDYTNACLREADEIAKQSNNISVITGCPTYNKTGRGKMLYNSALVMQ
                     NGKVQTEIHKALLPNYDIFDEYRYFEPGRDFSCVTVSGKKLAITVCEDLWNIEGKHLY
                     VADPMEMLSKENPDIIINIAASPFSSGQFEKRLEVFRYHTAKYNIPVVYVNMTGAQTE
                     LIFDGRSMIVFPDGSYKVLNAFEEDFFVFDTEIIPANKSEIKENKIALIHDALVFGIR
                     EYFTKLGFKKAVLGLSGGIDSAVVACLACEALGKENVDGLLMPSEFSSQHSIDDAVKL
                     AQNLGMRHEIVPIGKSFDAVNETLKPVFKDLPFNITEENMQARLRGLLLMAWSNKFGN
                     ILLNTSNKSEAAVGYGTLYGDMCGGLSVIGDLYKIQVYELAAYINRKKEIIPQNTITK
                     APSAELRPNQKDSDSLPDYNILDEILRLYIEEIQSPADLIARGFDKNLVARICKMVNT
                     NEYKRYQSPPALRVSSKAFGIGRRMPLAAKYLS"
     gene            complement(14947..15180)
                     /locus_tag="PKY63_05960"
     CDS             complement(14947..15180)
                     /locus_tag="PKY63_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPF00192.1"
                     /translation="MNKELEEKVKNVIEQIRPYLQADGGDIAFDRLTDDNVVYVHLQG
                     ACHSCPMALQTLKQGVEATMQRVLPEIKSVEQA"
     gene            complement(15193..16281)
                     /locus_tag="PKY63_05965"
     CDS             complement(15193..16281)
                     /locus_tag="PKY63_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPF00193.1"
                     /translation="MEKEIILGALKTVMDPDLKKDLVTLNMIDNLEVSDAKVSFSIVL
                     TTPACPMKEKMKNECIAAIHALYPDAQVEVALTAKVTSHKDKPALKNIKNIIAVVSGK
                     GGVGKSTVAVNLAAGLARAGAKVGLVDGDIHGPSIPLMFGLQNAHPQVVEKEGKQVII
                     PFEKFGVKLVSMGFFADPSKALIWRGPMIANAFTQIMNDTDWGELDYLVFDTPPGTGD
                     IHLTLVQNYSVTGIVVVSTPQEVALADARKALSMFVDQKVNVPVLGIVENMSFFSPPD
                     MPEKKYYLFGKEGARRLAEEHNIRLLAEIPLVESVSQSGDSGVPAVCDESNPLQKSFM
                     SMAENTAQAVAIRNATLDATQIVEITNK"
     gene            16410..19235
                     /gene="uvrA"
                     /locus_tag="PKY63_05970"
     CDS             16410..19235
                     /gene="uvrA"
                     /locus_tag="PKY63_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPF00194.1"
                     /translation="MKKDQKIIIKGARVNNLQNISVEIPAGRLTCITGLSGSGKSSLA
                     FDTLFAEGQRRYVESLSAYARQFLGRMLKPDVDFIDGIAPAIAIEQKSSTRNPRSTVG
                     TVTEIYDYLKLLYARIGRTYSPISGKEVKRQQVKDVIHYLQKLPPDTRIFLLTPYQVR
                     EGLTLTDALALSMQQGYSRILFNSETISIESFLDDLKKKKSIKKSDKLFLMIDRAVST
                     DDTEEIAQRLSLSIEAAFTEGNGICIIYTDHDKHYETFSKRFEEDGISFEEPSVNLFS
                     FNNPYGACPTCEGFGTIIGIDPNLVIPDKTLSVYDGAIAPWKGEKMGEWLIDFLNVAW
                     EFDFPVHKPFCELNDAQKNLLWTGNKHFEGLNAFFKMLEQNLYKIQYRVMLSRYKGRT
                     TCPDCRGTRLRNDASYVKIQGQSILDLVLLPIRNLADFFNNLKLSTYEMKVSSHILRE
                     INFRLKFLSNTGLEYLTLNRLSNTLSGGETQRINLARALGSSLVGSMYILDEPSIGLH
                     PVDTEKLIEVLKNLRDLGNSVIVVEHDEDIIRESDYIIDMGPGAGRNGGYVVFQGTMK
                     QLMQNKESHTAKYFRGEDQLPVKQKPLKPRFFIGLKNVDQNNLKNISVQFPLNAITAV
                     TGVSGSGKSSLVRQVLYPALQRHFGNYSEQSGSFGSIEGDIHLLTGVEMVDQNPIGRS
                     TRSNPATYLKAFDEIRDLYAALPLSKQRGYKPGFFSFNVEGGRCEVCQGEGTITVEMQ
                     FMADVHLVCEECGGKRYKSDVLDVQFNEKNISDILDLTVNEALTLFSSQINEKFGSHC
                     KRICDKIQPLADVGLGYLQLGQSSSSLSGGEAQRIKLASFLSKALPNESILFIFDEPT
                     TGLHYHDIQYFYQSITRLINAGHSVVLVEHNMELVKYADYIIDLGPHGGDRGGEVLYQ
                     GPLDGIKKIKKSATASAISKKLPEWAK"
     gene            19232..21847
                     /locus_tag="PKY63_05975"
     CDS             19232..21847
                     /locus_tag="PKY63_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF00195.1"
                     /translation="MNSIGKAFKFKALKVYASTEWVLDGKKYRKVFENMETTYLYAEL
                     SFYNKLFDEEDWTAEINLKCFAKETKGKNKELCNIQEKIEISKEKNEIYFRQGWGAEK
                     IGSFWKRGDYFWEAWIDGEMVGTADFHVEGEGLVTHENNPYFNLQSMRVFEGGWNFVQ
                     EKDRVYLKRFSKADTRYVWVEVNIENKAEKPWYCEIFLFFYDKARQLKGTTSMLNYVN
                     SSTDAKLSFATGWGNNTGGDTYKQDLYYVEVVFMENLLGTIPLHFEEKNEEGDVEVFA
                     PGLIQSNLQTGTAAKKENIEEVLAELDQLIGLTSVKKQIREHISYIDFISIRKEKGFQ
                     DSDKISLHSIFTGNPGTGKTTVVNLLGRIYKAMGLLSKGHVIEVGRAELVAEYIGQTA
                     PKVKKKISEARGGILFIDEAYALFRKDDEKDFGREVIEILIKEMSDGKGDIAIMFAGY
                     PTEMHDFLFSNPGLKSRINYSFHFDDYLPEELMKIVRHHAVKKKLIISTEADKEMNEV
                     IIAAYRNRDRTFGNARMALSLVDESKMNMARRLMLKPNVRDLSEEELSTIEVIDVKEV
                     HSKEEKKKLKLAVDEGLLNIALDDLNRLVGLNTIKTEMNELVKLVRYYNDLGKDVLNK
                     FSLHTVFTGNPGTGKTTVARIIGQLYKALGLLERGSVIEVDRSELVAGYVGQTAIKTQ
                     AVIESAMGGVLFIDEAYALTDNGGNDFGKEAIEVILKEMEDHRGEFALIVAGYPGPMS
                     KFLLSNPGLKSRFDRLLNFPDYTETELLDIARGMFEAEGLLMDSDAEKYFLTLIADML
                     KKRDEYFGNAREIRKITQTVIRHQNLRLASMESKLRTPQMIATIIREDFNDIELPTST
                     QSGVPIGFKRSEQNS"
     gene            21992..22435
                     /locus_tag="PKY63_05980"
     CDS             21992..22435
                     /locus_tag="PKY63_05980"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPF00196.1"
                     /translation="MEQQPNNHQNPNQQQNNYQPQQPQMTWEGIINDHIAKTRGWMMF
                     MGVFMIVYAALATIMSLGIGIVFAWIPFILGLFLINGANRAKSFLMTGSQADLADYHK
                     QMKNFFLTNGILMIVSIGLMIILILVAITVGINTSNMNFSKGFNF"
     gene            22467..22838
                     /locus_tag="PKY63_05985"
     CDS             22467..22838
                     /locus_tag="PKY63_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HPF00197.1"
                     /translation="MIQLSTGTIVFYLVIMIFLVIIPMWKIFTKAGQPGWACLVPIYN
                     VYVMTLIAKKPAWWMVMILIVPIANIIFGIMLLHAIVTNFGKGAGFTLGVIFLSIIFI
                     PILGYGSAQYNPPVAPAQPTM"
     gene            22917..23354
                     /locus_tag="PKY63_05990"
     CDS             22917..23354
                     /locus_tag="PKY63_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00198.1"
                     /translation="MAMKFKPSVYMIILFVLTSLATIAGLYLVVDSMILGEDVKNSLA
                     MLSKAITPAQYYAAVAILSLLIIMKWFGSFIMFRGKPWGFILYVIPNLILLAMMTYLI
                     IYNFRTENIYAFGGGTLAFIIAYLVAMIMIIKKRKALRKNIAG"
     gene            complement(23786..24208)
                     /locus_tag="PKY63_05995"
     CDS             complement(23786..24208)
                     /locus_tag="PKY63_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPF00199.1"
                     /translation="MKTRIFAMVAAALFVLPMITFGQTETKEKPKKVEEIKIQTNLHC
                     GSCKEKIEAELATVKGVKEAVADVDTKVVTVKFSPKKTSADVIVERIQKLGYQAKTMG
                     GCCPGHGSGSGCSGAGKTETGGCKGSGSGSGTGCGGKH"
     gene            complement(24226..24507)
                     /locus_tag="PKY63_06000"
     CDS             complement(24226..24507)
                     /locus_tag="PKY63_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPF00200.1"
                     /translation="MKTIIEIENLKCHGCANTITKSISSMDGVADVQVNHESSEVTIF
                     YEGQTDRTEEFARKLKTLGYPRVGENSAGRVVKSYISCAIGRMDSGKKN"
     gene            complement(24510..26729)
                     /locus_tag="PKY63_06005"
     CDS             complement(24510..26729)
                     /locus_tag="PKY63_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00201.1"
                     /translation="MIKIFLSVMVFTMLLTSGYAQISGVVYGESPSGNKVHLPAANVY
                     WSDKQEGVVSEMDGSFSIAEPPAGIGIIVSYVGFESDTVFDFSDGNIEIVLRESNILQ
                     QVDILETNAASHLERLNPLWTQQVGSGELQRAACCNLSESFETNASVDVNYTDAVSGA
                     KQIQLLGLNGVYSQLMIENIPFMRGLGSAFGLEYIPGTWMNSISISKGTSAVINGYES
                     ITGQINAEFKKPEAKEKFFFNYYLNDAGRHEFNINAAADLNKKVSTILLGHYSFNNSE
                     IDHNMDGFVDMNRGRQINAANFWQYISPKGLEMRWGVRLLDETRSGGQITGSPYFVVS
                     PEGLWKSEISNRRGEAFVKTGYIFKNKPGTSFGFINSFSVHDLDMLFGNKTYSGLQKS
                     FYSNFIFHSDFKETKHQYDAGFNFIYDNVSEQLNDSSWNTEEMVPGVFFQYTYTLPEK
                     ITVMAGLREDYNNRFGFLTTPRLHMKYHFNEKTTLRASAGLGYRSPYVIPENISLLTS
                     SRGIVFLNKPELEEALNYGATLVRYFDVLGKELMVSFEYFKTDFQHQMMIDMESDTSV
                     VYVYNLNGKSYSHNAQIEISYPVFRHLEVRTAFRYNDVQQSLRDGELQQKPLVSNYKG
                     LVTLAFASNMKRWQADATAQFIGKSRLPDRSEFPVPYQLDEYSPEHIVVHLQLARNFK
                     HWTIYAGAENVTDFKQHHPIISADKPYSSYFDSSVIWGPVMGRKIYAGVKIKFNKKD"
     gene            complement(26764..27219)
                     /locus_tag="PKY63_06010"
     CDS             complement(26764..27219)
                     /locus_tag="PKY63_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00202.1"
                     /translation="MKILRNSFVLVLIPLMVFSSGGFNIFKHMCHTTGEMMVSFQSPE
                     SCEHEYAEHSRTCNSSCCGHDEKETSCCENVHVFIRTVDNVSQSEERPEISNLSSLLN
                     PAVKIVFVDPEMKITKDYLSGDYSSPPELSGKSTVIKHHALKIDCCFTV"
     gene            complement(27286..>27615)
                     /locus_tag="PKY63_06015"
     CDS             complement(27286..>27615)
                     /locus_tag="PKY63_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00203.1"
                     /translation="LVLSLADYSILFIKPAFSKMASSPIKLGESLAMGVPVICNAGVG
                     DLGNIEKDGFGFVAEYTRDTDYMNICNRIAEKNFDKNDLRFRSAYGYDLTKNVHKYLQ
                     IYQKTGN"
BASE COUNT         8060 a         6240 c         5652 g         7563 t
ORIGIN      
        1 cgcttatatt tgcatcgcat cactaagaaa aaaacaagtt tttagaggtg cccttttgaa
       61 aactgaaaaa acaagcacat acatcaaaca atgactacag ggatcatcat aacactatgc
      121 gttcttattc ttatctccta tgtatttgat ctgacttcat caaaaacaag aattccctct
      181 gtcatacttt tgttgctgat tggatggctg cttcagttat ttgctgttca gttcggcatc
      241 aatgttcctg atctgagcga agcgctgccg gtagtaggaa ccataggact gattctgatt
      301 gtacttgaag gatcgatgga tctggagctc gacaaatcaa aaaaacgggt ggttttaaat
      361 tcagttttca tggctctgat tcccatgctg attctgggca caggtctcgc ttttattgtt
      421 caggaagttg ctgatgtttc attccggaca gctttgatca atgtaattcc gctatgtgtt
      481 atcagcagcg ccattgcgat accaagtgca caatcgcttt gcaaatcaaa ccgcgaactc
      541 gttacttatg aatccagtct ttccgatatt tttggggttg tacttttcaa tttcattgca
      601 ctgaacgaaa gctttggatg gaacaccttc ggcaactttt tccttcaaat actcatcaca
      661 ttggtcattt cttttgcagg cattataggt ctttcagtgc tgctgcactc cataacgcac
      721 catgtaaaat ttgtacccat catcatgttg ctcattttgc tgtatgccat caccaaagtc
      781 cttcatttgc caggactgat tttcatcatg ctctttggaa tttttctcgc caatcttcag
      841 ttgctgcaca aagtaaaatg ggtaaaagtt tttcatgtcg aatcgttgaa aaaagaatac
      901 ctgcggttta aagaaattgt cggcgaagga tctttcctga tccgcacctt gttttttctt
      961 cttttcgggt tcatgatcaa gaccgaagaa ttgctaaata ttcaaagtct cccctgggct
     1021 ctggctatta ctgcgggcat ttttatcatc cgatttgcat ttcttaaatt attgcggatc
     1081 aaagagatta cttttctata tatagcacca cgcgggctga taacaatttt attattttta
     1141 tcgatagcag aaagcgataa aatagcacta attaacacat ctcttgttat tcagattgtt
     1201 atatccactg cccttattat gatgtttggg ctcatgacca acaaaaaagt caaatcacca
     1261 gacattccgg ccgaaagcgc cagcgagcca atacacgaag agccccaaaa cggcgcaata
     1321 caataatccg gcgcaattca agttattttg ctaattttgc tggcttaata tgaataaggt
     1381 gaccatactt caactccaac aacttttgtc gaaaactgtt ctcgttgcaa tgattgtttc
     1441 aggcatcata cttacaggat gcaatgccga tgaacagctg ggactcgaca ttcagccacc
     1501 aggcgacgaa ctcaacgcag taaaatccga cacgttttca atcacggcat atacacgcca
     1561 agccgacagt gtgcgcaccg atgaaactgc aataagtctg gccggctatt ataccgatcc
     1621 ggttttcggt tcactccgtg cttctttttg ttcacaacta cgcctatccg gcaccgatgt
     1681 caattttgga aacaatgtag taatagattc cgttatctta catctggaat attacaacat
     1741 atacggcaac aacaaagaaa acaacagaat gacatttaac gtgtacgaac tggccaatgc
     1801 catttatgtt gacagcacgt attattcatt ttcgcccatt gccagcggtc gaaaacttgg
     1861 aagcatttca tttctgccaa acaacgaaga cagcatatat atcaatggta aaagactggg
     1921 gccgcaacta cgcattccgc ttgacaaaaa tctgggtaaa cgcatcgttg aggcatcttc
     1981 tatgggatat ctggcttcca acgtcctttt tgaagatttt tttcatggaa tcagcgttgt
     2041 tccgacagca gaaaacccaa acggctccat catctctttc gatgttttat cggccaattc
     2101 aggacttacc gtttattatc acaacgacag cgacaccctg agttataaat tcaatatatc
     2161 tacaaccagt gcgagataca gcagtttcaa ccatttttca tttaatggtg cgcatcccga
     2221 tttgctggga caggtaaacg gcgacaccgc tctgggtcaa caaaaattat atctgcagcc
     2281 attgggagga actgaaatat tgctcaattt tcctgacatt caatcactgt cagacaatgt
     2341 tttttctgtt gtcatcaacc gcgccgaact ttttattccc gccgatctga gcgacataag
     2401 caatgaaacc tatccgcggc cggtaaaact cacattggtt gaactggatg aaaacggcga
     2461 ctatgcttat ctgagcgatt atacaataaa ttccgaaacc tacggtggaa gttttgatga
     2521 aaatcttggc tgctaccgtt ttgttattac acgacatatg caagacatca tcacaggagg
     2581 agaaacgtct tatggatatg ttttgatggt gagcggaaca tcgatatatg gcaaccgcgt
     2641 aatactgaat ggtccggaaa gcacaaatcc gatgcgactt gaattaacgt acactcttat
     2701 tgaataagtt taaaaattat ctgttatgtg tggaattgtt ggttatctgg ggccacgtca
     2761 ggcctatcct attttgatta atggacttca tcgtcttgaa taccgtggct acgacagtgc
     2821 tggagttgcc ttactcaacg gagatttgcg tgtttacaaa agcatgggga aagtgcggga
     2881 tctcgaagaa cttgtcaaag ataaaaacct gagcggaacc atcggcattg cccacacgcg
     2941 ctgggctaca cacggagaac ctaatcatgt aaatgcgcat ccgcatattt ctccttcagg
     3001 aaaaatggcc atgattcaca atggaattat tgagaattat gccatcctga aagaagagct
     3061 tatcaaccgc ggttacaaat tcaaaagcag caccgacacc gaagtgctca ttcatctgat
     3121 cgaagatatt caaatcaacg aaaaaacaag tcttgtcgaa gctgtacaga tagcgctcag
     3181 ccaggtaatc ggtgcatatg ccatcgtaat cacctcgcag gatgatcccg acttgctgat
     3241 tgctgcaaaa aaaggaagtc ccatggtgat tggtatcggc tctgatgagt tttttatcgc
     3301 ctccgatgcc actcccattg ttgaatacac caaaaatgta gtgtatctcg aagatgaaga
     3361 aattgccatt ctggaacgtg gtcaggaact gaaaatcaag accattaaaa atatcgataa
     3421 aacgccatac gttcaaaagc tggagcttaa tctgggagcc atcgaaaaag gaggatatcc
     3481 gcattttatg ctgaaggaga ttcacgaaca gcccaattcc atccgcgata gtatgcgtgg
     3541 acgtttgtcg gctgaccagg gtattatttc actgggcgga attgtcgatt atgaacagaa
     3601 actctgcaat gccaaacgca ttatcattgt ggcctgcggg acctcgtggc atgccggtct
     3661 ggtgggcgaa tacctgatag aagatctggc ccgcatcaat gttgaagttg aatacgcttc
     3721 ggaattccgt taccgcaatc cggtgattac cgaagatgat atcatcattg ccatcagtca
     3781 gtcgggtgaa accgccgata cgctggccgc cattaaatta gccaaatcga aaggcgctac
     3841 catccttgga atctgcaatg tggtcggttc caccattgcc cgcgctaccg acgctggaag
     3901 ctatacccac gccggacccg aaatcggagt ggcatccacc aaggcattca cagctcaggt
     3961 gaccattctt accatgctgg ccctgatgct tggacacctc cgcggcacca tcagcaaatc
     4021 gaaatacaat cagtatattc acgagctgga ccgtattccg gaaaaagcaa aaatcaccct
     4081 cgaaaaagca cacggcatca tcgaaaaaat tgccgaacaa tacaaagatg ttcgcaactt
     4141 cctgtatctg ggacgtggct acaactttcc ggtagccatg gaaggagcac tgaaactgaa
     4201 agagatcagc tatatccacg ccgagggcta tcctgctgcc gaaatgaaac atggccccat
     4261 tgcactcatc gacgaaaaaa tgccggtggt tgtcattgca accaacaaag gaacatacga
     4321 caaagtagtg tccaatattc aggaagtgaa agcccgcgcc ggaaaaatca tttcggttgt
     4381 taccgaaggc gatacagccg tgaaggaaat gagcgactat gtgatggaaa taccggaaac
     4441 cgaagaattt ctggttccgc tgctggctac cattccgttg cagctgctgg cctatcatat
     4501 cgcagtgctc agaggttgca atgttgatca gccacgtaat ctcgccaaat cggtcacagt
     4561 tgagtagctc gcagagaaat ttgccacgga ggcaccaaga cacgaaaaat ttcttcgacg
     4621 atgaagcctt attctatgag tcgcgcagag ctcgcagtgc cgcagagaaa tttgccacgg
     4681 aggcaccaag aatattaaac atggaacctg tctgcaattt taactgcaac aggcagtcag
     4741 gcaggcaagg aagtttgaag taagaagtaa atcccgatta aagtaccgtt caccattctc
     4801 acttccttat tcaagattca gtgttcatga ggtgccggga tcttcccgaa aaccaacgac
     4861 gcaacaactt cggcgctttg cttgtccgcg aatgaacacg aagtaatcat cttccctcag
     4921 catgtctctt atgaacgcta acccgccagc ataagtcact ggcgtgacat gatgggcggc
     4981 caagtgtgaa tccgccttca cctgtctgca acaggcaggc ctgcggtgaa gactgattca
     5041 gtgggtcgcc ctgaaatctc gcgcagagag cgcagaagac ttggtttgtt tatgcccttt
     5101 gaccctaaat cactgaacga aataagaaat ctgcataaag attattgcct gattctaatc
     5161 tatcagactg agcaatatat attccagttc cctgcgggca ctgatagcct tttcgatacc
     5221 gaggctgcag aggtgaactg cttttcggtc ttttcaattg ttgaaatcta actctacaag
     5281 ctctttcagt gtttcgaaat atccatcctg cattgcaagc aatgtctgaa gttccagttg
     5341 ttttttgtcg attgtttcca ttatcatacg aattgcaaaa caaatgtatt gaacgaatgt
     5401 tatttcatga agaatttggt gttatcaata caataacaag agactgtctg aaaacagctc
     5461 cttcgtcgct tgcgaaagtt tattccacta cggaagtgga atggtattct tgcctgcagc
     5521 accaaattca gacagattct aaaaaaacta aaataaaatt gcttttgtgt tgaaaatttt
     5581 ttaatctcac atatttaatt ttttctctgc aagatatctt gatttatata ctaaatatga
     5641 atattactca cattctacaa cgtaaataat ctacattttt atatgtttaa tttcctatta
     5701 ttagtaaatt gcgaccaaat tctatctgta cggattaaac ttttagccaa tggtctaatc
     5761 ttacagaaaa tttagaaaac ttaattcggg ggaattaatg ggggaaatat ctgtcgacaa
     5821 acaatcctgc aacatcttgc ttgccagcca atatggcctc agcaagcgcc ttgttttttt
     5881 aaacagaatt gcatcctttt tattcccgat tgaaataaaa aaaacgaaca tgcaacccaa
     5941 aatcttaacc atcttaattt tattcctcag tctgaatgtc ttcgggcaaa acaactcgac
     6001 acaagacaca aaaaccatta acggatttat tgtccctgtt gatttgctcg acccggccta
     6061 tccgataatg aaaaataccg gcgacccagt taaagatgct gaagattttc gtcttgcatt
     6121 aaggatttac tcaaaaaatc ttgaccgttt ccctgtttat aaaaacagcg gagatgaggc
     6181 aaaagacgaa gccgcttacg atgaagcatg caaaactttt tttgccagac acccctactt
     6241 tccacaaccc atcaatacca ataacccacg tttggatgaa cagaatttcg accgatggta
     6301 caaagcatac ctgaaattct atcccgaaaa agcaaaaaga gttgctgtta ttgaggaagg
     6361 aggtgtgaaa tgagaagtat tttgacattt gttttcagcg tgttttcatt gtatttgttt
     6421 tcacagacaa caacatttac aacaagcgga acatggacat gccctgccgg ggtgacctct
     6481 gttactgttg aatgctgggg cggcggcggt ggtgggggtg gaacaaaaaa cgattacaaa
     6541 tatggcggtg gcgctggtgc tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnntggtgct ggatcaaccg gtgctggagg aaatgcatct ggaactacgg caggatctgg
     6721 gacaacaatt gatggaggta acggagcatc tggatccaca acagaatcag atggttcatc
     6781 aggttttaca tatggcgggg gtggtagtgg tgcctttgta ccagacaata caaatcatac
     6841 aggaggggcc ggagcaaaag gattggttaa gataaccccg gtgtatgcag caccggcaaa
     6901 cgacctttgt gccaatgctg tttcgctgcc ttgtgcaacg accaatctgg caggaactac
     6961 ggttggatgt gtggcagaaa cttctcctgg cggctgtggt tcccaatatg gtgtatggta
     7021 taagtttacc ggagatggtc agcaaaccac catttcatgc actgcaacca gcagctggga
     7081 tcacgaaatt gacattttca gcggaacctg cgggacttta acaacagtaa gctgcgttga
     7141 cggagcttta tcagcaggaa cagaatcata tacctttaca acagtaaatg gtactacata
     7201 ctatgtttat gtttgccaat attcaacaag ctccacggta acaggacctt tcactatttc
     7261 cagaacctgc actgctgcag gagcagcttg tataccgaac acagtaattg catctttgcc
     7321 ttattcacaa acgggaatga caaccaacgg agccggagat gatttcagca gttcagatgc
     7381 atgcggtagc agctacatga atggtgacga ctatactttt tcatacacac cagcagcaaa
     7441 ccaatgcatt tctgttacac tcaccaatac tgatacatgg gtaggcgttt ttgttaccca
     7501 gggttgtccg cagggaggaa cgtgtgttgc aagtaccacc agttcgacag gaaatccgag
     7561 tttaagcttt gtctcattaa caggaggtgt tacctatttt attacgattt caacataccc
     7621 ctcacctcaa ttcaccccgt ttgacatatc gattcaaagc attacctgcc cgacaccccc
     7681 tgcaaatgat gattgtagtg gggcagtttc attgacatct aaccaatact catgcaccag
     7741 ctttgcaagc ggcactgttg tcggagccac cggatcttct caaggaaaca cctgctcttc
     7801 aacatatgac gatgatgatg tctggttttc atttgttgcc agtagcacag ctcatcaaat
     7861 tttaatatca aatattacag gttcatacac gtcgatgtat tttgctgtgt attccggcac
     7921 atgccctact ctcactgaag ttgattgttt ttatgatgaa tctggaactg taacaggttt
     7981 aacaattggt catacatatt atgtaagagt ttatactgca acagcaactc cgcttcaaac
     8041 aacaacattt gatatttgtg ttacaaaatt atgtaatgca ccaacttcta ttattacttc
     8101 tgtttcaccg gttgggccaa ttacatcagc aacttctgtg acatttgacg tggattctca
     8161 tactggagga gcttgtgatg gcagttggga ataccaatgg gaaactacat ctggaacagt
     8221 cttacaagca tggagtacta catcagcata tacagccaca atcagttcaa cccaaaatct
     8281 tcgtgtaatg atgcgttgca gcagctgccc aaccagcacc aactcaggta cagccagcat
     8341 tatgtttgtc aatccgatga caaacaatga gtgcagtact gctgtttcct tacccgttgg
     8401 aaccagttgc aattatgaaa catatacact cgatgacgga ccgcatgccg ctacagcttc
     8461 atccaacgat cagccaacct gcactgccta cgatgcaagc cctgatgttt ggtttagttt
     8521 tacagttccg gcttcaggaa attatgttgt agatgtcagt ggtggcaccg gaatggatga
     8581 agttttctgt ttgtattctg gcactgactg taattccttg acttttctca catctcctgg
     8641 ttgtgttgat tatccaccaa caggaacttt atcaggaact cccggtgaaa ccatttgggt
     8701 cagagtttgg gaatactatg gggataattt tggcaatttt gatatatgtg tcaaagaaat
     8761 tacagctgcg acattcactt ctggacaaga ttgtgagttg ggatatacag tatgcagcga
     8821 tcaggaattt acaggcaatt caagtggcgg aggagttgaa gaactggatg caacaaacca
     8881 gggctgcatg tcaaccgaac acgaatcttc gtggtacctg ttcagcccgc aatcggcagg
     8941 cactattgaa cttgccatta cgcctgatgt ctgggccgac gattacgatt ttgccatctg
     9001 gggtccatat ccagaaggat caatggcttc cgatgtctgc ccgccaaccg gtgaaccgct
     9061 tcgctgttca tggtcggccg ctgacggaac aaccggacta attgattccg ctgctgttac
     9121 tgatgacaca gaagatgccg gcggagataa atgggtcaat gaaatcgttg ttactccttc
     9181 cgatgtaaac agtgtttatg ttcttttact tgacaacttt acaacaagtt cagagccatt
     9241 tacgctcaac tggactctga ccgatgtaat tcttgactgc acagtgcttc cggttattct
     9301 tctgaatgcc tatgcggaat gttcaactga cggcgtatta ctgtcctgga caaccgtaag
     9361 tgaaaccaat aacagtcagt tcatcattct cagagatgat ggatccggag attatattga
     9421 aatcggaaga gtatcaggtg caggcaactc aaacatgaaa cagttttaca ctttttctga
     9481 tcattttaaa ggagaaggca ctgtttacta ccgtttagat cagattgact ttgatggcaa
     9541 acaaactacc ttaaaaacca tgactgttga ttgttatcat ttgtccggtt ttaatatcaa
     9601 cagcgctagt ttagatgcaa atgcaggtaa tctggaaatc aactttgaag gtttcgccaa
     9661 tcaggagtat caacttaacc ttatggacct ttcaggaaaa gtgattttca gcgattcatt
     9721 cattgctgcc aatgatggta aaaaccaggc tgtgtcttat ttcaaaccgg ttgcacaggg
     9781 cgtttatgtg gtttcgctga ccaataaatc aggaattacc aaattttcca aggtttcacc
     9841 aagataagac agattcaatt ccttataaaa aacaccccgt ctcccaaaaa ggcggggttt
     9901 tttttcatat cccaaacttc ctcctatcca gcaatatcgc gccgtcttca tagatgcgca
     9961 cttcataatc gccggaaggg taatctgaaa catcgagatc aagggtgaag gtgtatctgc
    10021 caatgccatg aatggcaggc tccatcagcg ttttgatcag tctgccatta ttatcagtca
    10081 gtatgattgt aattgggcaa ttgtaccgca cataataatg aatgtcgcca cggatgcggg
    10141 ttacagcacc agtaaaaacc acggttgaat cttgtttgta ttgaacagtc gtccagggca
    10201 tttccttgtc agtcaggtcg cacagtattt ttcgcaaagc tattgtctgt tcccggctac
    10261 cgcaatcgac accggcgata tcgtgaccat ttgaaatacc ccaaactgcg tgttgcactg
    10321 ctccatctgg aaaactgttc ctgtcaatga atgaagccag ttttacccat ggctccgggg
    10381 caacaaaacc aacgtcgtac cccatatcat aaatcggtga agaattactg ctttcacaac
    10441 aaaaaccacg gattttaatt ttcgtctctg agtttggggc aatggtcaca taatacgatt
    10501 tcacattgag caggtcctga caacgcgctg tgtcagcaat aaagcggcgt ccgggttcca
    10561 ccagcacatt catgggttga ttggtgttgt ttttcatcgc ggcttcaatg aacatcgcct
    10621 ccattgagcc aatatatctg gctgaaaatt cgaagctgga attttggagg attgttccca
    10681 gaggagtata ttgaacaccg ctctggttct gcgaaacaac agatccaatc actgaacaga
    10741 tcagaataaa aaacacagat attgatttca ttttggatac attaatgaga gtacaacgat
    10801 ttttggaata cagatattat attgtggcgc aacgctctaa acgatttaca aaaacagtcg
    10861 caagagttgt cacgatttaa tgattcttcc cgacagcttg tccgcccact ggcggaattc
    10921 caggtcagca ggtctgtttg tcaacccttt cttgctggag tttaccacgc attaggcggg
    10981 gctaaaatgt cgggaatgtc cggaagttat agtttcgccg cggcgcgatc cagagcaaat
    11041 gggcacgcgt ctcctgctag cagcgttcaa cgttgacgcg tgacctgcaa tctattccgc
    11101 cttctccggc ttatagcccg atttattcag aatttcctgc gaattgttgt tgttcagcaa
    11161 ttgttcaaaa gtgatatctt tattttttgc catataggca cgaacaatct gccaaccgat
    11221 ataacgtccg atgcggggag cgctggtttt agaaaaagcg cttgtaaaag gagcttcgcc
    11281 aataaattta ttgatatcgc gcatttcggt ggtatgcaaa agttttttct cgataaaaaa
    11341 cccccagaca ttgccttgat ttttttcggc ccagtacagc tgctggccag agtatccaat
    11401 acgcactgaa tcggcgcaat caggcacagt cacatcggta aaatacaaca acttcccttc
    11461 aaaaatcatt ttatcaagca ttgtttttcc tgcaggactg tgcttctcaa gccaggcacg
    11521 acaaatttcg cttgcacatt tcaccaccag aaaatctttg gccatacggt tggtaatata
    11581 ttgaggaaca ccccacatct tgtaaatcgg atatttttca cccaaaaaaa gatcaagtgc
    11641 aatgacaaga ttatcatcgg catacttcac cggatattcg atatctccac ccgaaacata
    11701 agtataaaca gtgggaactt tccacaaggg aagcagatat ttaatgcgcc tgaaagtcat
    11761 tgtaagctcc gattcaagcc atgttaatga tggatactgt ttcatcgttt cgcgatacaa
    11821 cgaaagaatg gtcggatctt tcagatattg ctgcaaatag gcaatattca ccggatcatt
    11881 caagtcttca ggagaataaa aaaatgaata ttttttctgc atcgaagtca atccttcttt
    11941 cagactatca acaggaatgg caaacaaatc cttctcatag cgattaatac tcactttgat
    12001 gaatgaaagc gaatcaagtt tgctcccaga cacttcgcgg tcgaattcct tcttatcgcg
    12061 atcgcattta catgatccca tcatcatcat cgataatgcc aacccaatga ctaacaacaa
    12121 tctgaaggcc gatgcaaaaa attttgttcc attcataatt ttactaactt tgtgctacat
    12181 tgaaataaaa cccaaaagta tgaaaaagat tgtattggct ctgattcttt tcgcagcatt
    12241 cgcttttaca tccaatgccc agaatgaaaa attcttccgt ttcggattac atgcaaacac
    12301 aggattaacc tggatgaaac ccgatcagga ttcactggaa tatcagggca tgaaaatcgg
    12361 acttggctgg ggttttatcg gcgaactcac tattgcagac aatttttctt ttgccaccgg
    12421 tttcgatata aattatatgg gcggcaaact tcaaaaaatc ggtgtaaatc agatttatcc
    12481 acccgataca gctgcagtat tttccaaact cacatcgaat tatcgttttc aatttctcga
    12541 tattcctctc acactgaaaa tgaaaacaaa tgaaatcaat tatatcactt attttgcacg
    12601 catcggcggc tccattggta tctgcctgaa tgccaaagct gatgaggaat acagcccact
    12661 gagcggatca caaatgcttt cactcaacga tcagaatctt aaagaagaac gcaacttttt
    12721 ccgcgccaac ttccttatca gcggaggtct tgaatattca ctcggcggaa caacttctgc
    12781 ccttgccgaa ttatcctttg taaacggcct cacctatcaa ctcgaagata acaaatccat
    12841 tggcaactac atcatgctga aatttggcat catgttctaa aaaaatattt gtttacacat
    12901 catcccggtt ccgaagaatc gggatttttt ttaaacagtt ttatcatgaa aatcgctctc
    12961 tgtcagataa acacgcatac cggacatttc gcatttaacc gaaaaaaaat cctggaatac
    13021 attgaaaggt caaaacttga aaatgctgat ctggcggtgt ttccggaact atgcatatca
    13081 ggatatccag cacgcgattt tctcgacttt gatgattaca ccaacgcttg tttgcgcgaa
    13141 gctgacgaaa ttgcaaaaca atccaacaac atttctgtga taaccggttg tccaacttac
    13201 aataaaaccg gccgcggcaa aatgctttat aactcagcgc tggtaatgca aaacggaaaa
    13261 gtgcaaacag agattcacaa agcactgttg cccaattacg acatctttga tgaataccgc
    13321 tattttgaac caggtcgcga tttttcgtgt gtaacagttt ccggaaagaa gcttgccatc
    13381 acggtttgcg aagacctttg gaacatcgaa ggcaaacatt tatatgtggc agatccaatg
    13441 gaaatgctgt cgaaagaaaa tcccgacatt atcatcaaca ttgctgcatc gcccttcagc
    13501 agcggacaat tcgaaaaacg tctcgaggta ttccgctatc atacggcgaa atataatata
    13561 ccggttgtgt atgtaaacat gaccggggcg caaaccgagc tcatcttcga cggccgctcc
    13621 atgattgtct ttcccgatgg cagctataaa gttctgaatg cttttgaaga agactttttt
    13681 gttttcgaca ctgaaattat tcctgcaaat aaatcagaaa ttaaagaaaa caaaattgcg
    13741 ctgatacacg atgcgctcgt gtttggcatc cgtgaatatt ttaccaagct cggatttaaa
    13801 aaagcggttc ttggcttatc gggtggcatc gacagcgctg tggtggcctg tcttgcatgc
    13861 gaagcactcg gaaaagaaaa tgttgacgga ttactcatgc cttctgaatt cagttcacaa
    13921 cattccatcg acgatgctgt gaaactggct cagaatcttg gaatgcgaca cgaaatcgtt
    13981 ccgatcggaa aatcgtttga tgctgtaaac gaaacactga agccggtttt caaagatcta
    14041 ccgttcaaca tcaccgaaga aaacatgcag gcacgtttac gtgggttgct gctgatggcc
    14101 tggtcgaata aatttggcaa tattctgctg aacacctcca acaaaagcga agcagcagtg
    14161 ggctacggaa cgctctacgg cgacatgtgc ggcgggctga gcgtgattgg cgatctatac
    14221 aaaatacaag tatatgaact ggctgcctac ataaatcgta aaaaagaaat cattccacaa
    14281 aatacaatca ccaaggcacc atctgcagaa ctgaggccaa atcagaaaga cagcgattca
    14341 ctgcctgatt acaacatcct tgatgaaatt ttacggctgt atattgaaga aattcaaagt
    14401 cctgcagacc tgattgcacg cggctttgac aaaaaccttg ttgcacgcat ctgcaaaatg
    14461 gtgaacacca acgaatacaa gcgctatcag tctccgcccg cgctgagagt gtcttccaaa
    14521 gcattcggca taggccggag aatgccgtta gctgcaaaat atttgtcgtg atttttttcg
    14581 ttacacgtgg aatttatcac gctgaaagcg gaaggagtta cgcgttacag acgttacaag
    14641 cgtgacacgt tacaaaactc agatcacgcg tcaggagacg cgcgcccaga cagatgttac
    14701 gttgtaatca gggtaggcaa ctttgtgctt cttcaacctc tctgcgtcgt gactgtgtca
    14761 cgtttcacgt gaagcgacct gagtgcgttg aagaaataac atcattcgac gtagtttatc
    14821 ctgcaggacg caggactcag agtgacagat gcggggctca gacttcgcat gccgcaagca
    14881 tttacgtcgt ctttaaactt tacaacttta ttactttcaa ctcgcgaagc gcaaagcgct
    14941 ttgcacctac gcctgctcca ccgacttgat ttccggcaac acccgctgca tagtagcttc
    15001 cacgccctgt ttcagcgttt gcaacgccat cgggcaacta tgacaggcac cctgtaaatg
    15061 cacgtaaaca acattatcgt cagtcaggcg atcaaatgca atgtcaccac catcggcctg
    15121 cagataagga cgtatttgct ctataacgtt tttcactttt tcttcaagtt ctttattcat
    15181 acgcgacaaa ttttatttgt tggtaatttc gacaatttga gtagcatcga gggtagcatt
    15241 acgaatagct acggcctgag cggtattttc ggccatgctc ataaatgatt tctgcaaagg
    15301 attgctttca tcgcaaacag caggaacgcc actatcgccg ctctgactca ccgattcaac
    15361 caatggaatt tcggccagca ggcgaatatt gtgttcctcg gccaatcgtc ttgcaccttc
    15421 tttgccaaac agataatatt tcttttccgg catatcaggc gggctgaaaa acgacatatt
    15481 ttcgactatt cccaaaaccg gcacatttac tttttgatca acaaacatac tgagtgcttt
    15541 gcgggcgtct gccagagcca cttcctgagg agttgacacg accacaattc cagtaacact
    15601 gtaattctga acaagtgtaa gatgaatatc gccggtaccc ggaggcgtat caaaaacaag
    15661 ataatccagt tcaccccagt cggtatcatt catgatttgc gtaaaggcat ttgcgatcat
    15721 aggaccgcgc cagatcaacg ctttggatgg gtcggcaaaa aagcccatcg acaccagctt
    15781 tacgccaaat ttttcgaatg ggataatcac ttgtttgccc tctttttcaa caacctgcgg
    15841 atgcgcattc tgcagtccaa acatcagagg gatgctgggt ccgtgaatat cgccatccac
    15901 cagcccgact tttgcgccgg cacgggcaag tcctgcagcc agattcacgg caaccgttga
    15961 cttccctact ccaccttttc cggaaacaac agcaataata tttttaatat ttttcagcgc
    16021 cggcttatct ttgtgagaag tcaccttagc ggtaagagcg acctcaacct gagcatcggg
    16081 atagagtgca tgaatagctg caatacactc gtttttcatt ttttccttca tcggacaggc
    16141 cggagttgtc aatacaattg agaacgatac ttttgcgtcg cttacttcaa gattgtcaat
    16201 catgttcagg gttacaagat ccttcttcaa gtcaggatcc atcactgttt tcagcgcccc
    16261 gagaattatt tccttttcca tttttttatt aattcaatag ttatgacaaa ggtatgaaat
    16321 ataaatcaat tgtttttgtt tcaccatcag caaaagaatg ataattaaca tggaatgata
    16381 aagtcaattt tttctacctt tgttacttta tgaaaaaaga ccagaaaatc attataaaag
    16441 gcgcacgggt aaacaatctg caaaacatct ccgtcgagat tcctgctggc cgcctcacct
    16501 gcattacagg attgtcgggt tccggaaaaa gttcgcttgc ttttgataca ctttttgcag
    16561 aaggacaacg ccgttacgtt gaaagtctga gtgcctatgc ccgtcagttt ctggggcgca
    16621 tgctcaaacc cgatgttgat tttatcgacg gcattgcccc cgctatagcc attgagcaaa
    16681 aatcatcgac acgcaatccg cgttcaacag ttggaactgt tactgaaata tatgattatc
    16741 tcaaactact gtatgcccgc atcggacgca cttattcgcc catcagcgga aaagaagtaa
    16801 aacgtcagca ggtaaaagat gtcatccatt atcttcagaa attaccgccc gacacacgca
    16861 tttttcttct tactccctat caggtcagag aaggattaac attgaccgat gcccttgcac
    16921 tcagcatgca gcaaggatat tcgcgcattc tcttcaacag cgaaaccatc agtattgaat
    16981 cctttcttga tgatttgaaa aagaaaaaat ccatcaaaaa atcggacaaa ttatttctga
    17041 tgatcgatcg tgcagttagc acagatgaca ccgaagaaat agcacaacgc ctgtctctca
    17101 gcattgaagc cgctttcacc gaaggcaacg gaatttgcat catttatacc gatcacgata
    17161 aacattacga aacattttca aaacgattcg aagaagacgg aatttcgttt gaagaacctt
    17221 cggtcaacct gttttctttc aacaacccat atggtgcatg cccgacctgt gaagggtttg
    17281 gaaccattat aggtatagac cccaaccttg ttattcctga taaaacactc tccgtttacg
    17341 atggtgccat tgctccctgg aaaggtgaaa aaatgggtga atggctgatc gattttctca
    17401 atgtagcatg ggaattcgat ttcccggttc acaaaccgtt ttgcgaactc aatgatgcac
    17461 aaaaaaattt actgtggacc ggcaataaac atttcgaagg attgaatgca ttttttaaaa
    17521 tgctcgaaca aaacctgtac aaaattcaat accgcgtaat gctatcccgt tacaaaggac
    17581 gaacaacctg ccccgattgc cgcggaacac gcctgcgaaa cgatgcttca tatgtaaaaa
    17641 ttcagggtca atccatttta gatctggtac tcttgccaat ccggaatctt gccgattttt
    17701 tcaacaacct gaaattatca acgtacgaaa tgaaggtttc atctcatatc ctgcgcgaaa
    17761 tcaacttccg gttgaaattt ctcagcaaca caggtctcga atatcttact ttaaaccgtt
    17821 tatcaaatac cctttcaggc ggtgaaacgc aacgcatcaa tctggcacga gcactcggaa
    17881 gcagtctggt cggatccatg tacatccttg atgaacccag cattggacta catccagttg
    17941 atacggaaaa actcattgaa gtacttaaaa atctccgcga tctcgggaat tcagtgattg
    18001 ttgttgaaca cgacgaagac attatccgcg aatcggatta catcatagat atgggaccag
    18061 gagctggccg caatggtggt tacgttgttt ttcaagggac aatgaaacaa ctgatgcaaa
    18121 acaaggaaag ccatactgca aaatatttca gaggtgaaga tcaactacct gtcaaacaaa
    18181 aaccgcttaa gcccagattt tttatcggac tgaaaaatgt tgatcagaat aatctcaaaa
    18241 atatcagcgt tcaatttccg ctgaatgcca tcactgcggt aactggtgtc agcggaagcg
    18301 gaaaatcttc acttgtaaga caagtgttgt atcctgcact tcaaagacat tttggtaatt
    18361 attccgaaca gagtggcagc tttggttcca ttgaaggaga tattcacctg cttacagggg
    18421 ttgaaatggt tgaccaaaac cctataggcc gctctacgcg ctcaaatcca gctacctacc
    18481 tgaaagcatt tgacgagatc cgcgacctgt atgccgcatt gccactgtca aaacaaagag
    18541 gttataaacc cggatttttc tcattcaatg ttgagggcgg acgctgtgaa gtgtgtcagg
    18601 gcgaaggaac cattactgtc gaaatgcaat ttatggccga tgtccatctg gtctgtgaag
    18661 aatgcggtgg caaacgctac aaatccgatg ttcttgatgt acagtttaat gaaaaaaaca
    18721 tttccgatat tcttgacctg actgttaatg aagcattgac attgttcagc agtcagatca
    18781 atgaaaaatt tggttctcac tgcaaacgta tctgtgataa aattcaacca ctcgccgatg
    18841 tcggcctcgg atatcttcag ctgggtcaat ccagcagctc cctctcaggt ggcgaggcac
    18901 aacgcatcaa gctggcctca ttcctctcaa aagcattacc caacgaatcc attcttttca
    18961 tttttgatga acctactacc ggattgcatt atcacgatat tcagtatttt tatcaatcca
    19021 tcacccgcct catcaatgcc ggtcattctg tagtgctggt tgaacacaac atggaattgg
    19081 ttaaatacgc cgattatatc atcgaccttg gacctcatgg tggtgaccgt ggcggagaag
    19141 ttctctatca ggggccattg gatggaataa aaaaaataaa aaaatcagca accgcttctg
    19201 ccatatcaaa aaaactacct gaatgggcaa aatgaattca attggaaagg cctttaaatt
    19261 caaggcacta aaagtttatg ccagtaccga atgggtactg gatgggaaaa aatacagaaa
    19321 ggtttttgaa aatatggaaa caacctatct gtatgcagaa cttagcttct acaacaaact
    19381 ctttgatgaa gaagattgga ctgcagaaat aaacctcaag tgctttgcca aagaaaccaa
    19441 aggaaaaaac aaggaattat gcaacattca ggaaaaaata gaaatcagta aagaaaaaaa
    19501 tgaaatctat tttcgtcagg gatggggtgc agaaaaaatc ggttcgttct ggaaacgcgg
    19561 agactatttc tgggaagcat ggatcgacgg agaaatggtt ggtactgccg attttcatgt
    19621 agaaggagag ggacttgtta cgcatgaaaa caatccgtat ttcaaccttc aatccatgcg
    19681 cgttttcgaa ggcggatgga attttgtgca agaaaaagac cgtgtgtatc tgaaacgttt
    19741 ttcaaaagcc gacacgcgct atgtctgggt tgaagtaaat atcgaaaaca aagcagaaaa
    19801 accctggtac tgcgaaatat ttctgttttt ctacgataag gcacgtcaac tgaaaggcac
    19861 caccagcatg ctgaattatg tgaattcgag caccgatgca aagctgagtt tcgccaccgg
    19921 ctggggaaac aatacaggag gcgacacata caaacaagac ctgtactatg ttgaagttgt
    19981 tttcatggaa aacctgctgg gcaccattcc tctgcacttc gaagaaaaaa acgaagaagg
    20041 cgatgttgaa gtttttgcgc caggacttat tcaaagcaat ctgcaaacag gtactgctgc
    20101 caaaaaagaa aatattgaag aagtacttgc tgaactcgat cagctcatcg gactcacttc
    20161 ggtaaaaaaa caaatccgcg aacacatcag ctatatcgac tttatcagta tccgcaagga
    20221 gaaaggattt caggattccg ataaaatcag tctgcacagc attttcaccg gcaatcccgg
    20281 tacaggaaaa acaactgttg tcaatctgct tggacgcatc tacaaagcca tgggcttact
    20341 atcgaaaggg catgtgattg aagtcggacg cgccgaactg gtagccgaat acattggcca
    20401 gacggcacca aaagtaaaga aaaaaatcag cgaagcgcgc ggcgggattt tgtttattga
    20461 tgaggcctac gcgctgttcc gaaaagacga tgaaaaagat ttcggccgcg aagtaattga
    20521 aattctcatc aaggaaatgt cggatggcaa aggtgatatc gccatcatgt ttgcaggtta
    20581 tccaaccgaa atgcatgatt ttcttttctc caatcccggc ctgaaaagcc gcatcaatta
    20641 ttcgttccat tttgatgatt atctgcccga agaactgatg aaaattgtca ggcatcatgc
    20701 ggtgaaaaag aaattgatta tttctactga agccgataag gaaatgaatg aagtcattat
    20761 tgcagcatac cgcaaccgcg accggacctt tggcaacgcc cgcatggctt tgtcgctggt
    20821 ggatgaatca aagatgaata tggcccgcag actcatgctg aaacccaatg tacgcgacct
    20881 gagcgaagaa gaactttcaa ccattgaagt gattgatgta aaagaagtcc attcaaaaga
    20941 agaaaagaaa aaactgaagc tcgctgttga cgagggtttg ctgaatattg cactcgacga
    21001 tctgaaccgg cttgtcggac tcaacaccat caaaaccgaa atgaatgagc tggtaaaact
    21061 ggttcgctat tacaatgatc tcggcaaaga tgtgctgaat aaattttcgc tgcacactgt
    21121 tttcactggc aatcccggca caggtaaaac tacggtagcc cgcatcatag gccaactata
    21181 caaggcgctc ggcttgctcg aacgcggcag tgtgatcgaa gtggaccgct ccgaactggt
    21241 ggccggctat gtcggtcaaa ccgccatcaa aacacaggcg gtgattgaaa gcgccatggg
    21301 cggcgtgttg ttcatcgacg aggcctatgc gctgaccgac aatggtggca acgatttcgg
    21361 caaggaagcc attgaagtca ttctgaaaga aatggaagat caccggggcg aatttgcgtt
    21421 gattgtggct ggttatcccg gaccgatgag caaattttta ttgtcgaatc ccggcctcaa
    21481 atcaagattt gaccggttgc tgaattttcc ggattacacc gaaaccgagt tgctcgatat
    21541 tgccagaggc atgtttgaag ccgaaggatt gctgatggat tccgatgctg aaaaatattt
    21601 tctgacactg attgccgaca tgcttaaaaa gcgtgatgaa tatttcggca atgcgcgtga
    21661 gatcagaaaa atcacccaaa ccgtcattcg tcaccagaat ctgcgtctcg cctcgatgga
    21721 aagcaaatta cgcactcctc aaatgattgc aactatcatc cgtgaagatt tcaacgacat
    21781 cgaactacct accagcacac aaagcggtgt cccgattggt tttaagagga gcgagcaaaa
    21841 cagctgaaca aaaccatcat aggcctgaga ctgcattttc gaagtcagca ttgatccctt
    21901 gcatcttttt ttcttaaaca acacttaaca cagcgtgctg attgttaaaa tcgaaaattg
    21961 ttatatattt gccttattaa cataattcac catggaacaa caacccaaca accatcaaaa
    22021 cccgaatcaa caacaaaaca attatcaacc tcagcaaccc cagatgacct gggagggaat
    22081 catcaatgac catattgcca aaacccgtgg ctggatgatg tttatgggcg tattcatgat
    22141 tgtttatgcg gcactggcaa caatcatgtc tttgggaata ggaatcgtct ttgcctggat
    22201 tccgtttatt cttgggttat tccttattaa tggtgcaaac cgcgccaaat cattcctgat
    22261 gaccggttca caagccgatc tggccgacta tcacaaacaa atgaaaaact ttttcctcac
    22321 caacggaatc ctgatgattg tcagcatcgg actgatgatt attttgattc tggttgccat
    22381 tacagttggc atcaacacca gcaatatgaa tttttccaaa ggtttcaatt tctgacaaat
    22441 actaactcac ctaaaaaccc aaacctatga ttcaattatc taccggaacc attgtttttt
    22501 acctggtaat catgattttc ctggtaatca ttccaatgtg gaaaattttc accaaagccg
    22561 gtcaacccgg atgggcatgt cttgtgccaa tttacaatgt ttatgtcatg acactgattg
    22621 caaaaaaacc agcatggtgg atggtcatga ttcttattgt tcccatcgcc aacattattt
    22681 tcggcatcat gctgctgcat gccattgtta caaacttcgg gaaaggtgca ggatttacac
    22741 ttggtgtgat tttcctgtca atcatcttca tcccgattct gggttatgga agtgcacagt
    22801 acaatcctcc ggtagctccg gctcagccga cgatgtaatt cttttaaaaa actgcctcac
    22861 aaaggcagtt ttttttttga tttttttatt tagctttgat gtcaaattta accaccatgg
    22921 ccatgaaatt caaaccttcg gtttatatga ttatcctctt tgtgctcact tcgctggcca
    22981 ccattgcagg attatatcta gttgttgact ccatgattct gggcgaggat gttaaaaatt
    23041 ctcttgccat gttgtcaaaa gcaatcactc ctgcgcagta ttatgcagcg gtcgccattc
    23101 tcagtcttct cattatcatg aagtggtttg gatcattcat tatgtttcgt ggcaaaccat
    23161 gggggtttat actttatgtt atccccaatc tgattctgct ggccatgatg acttatctga
    23221 ttatttacaa tttcagaaca gaaaacattt acgcattcgg cggcggaacg ctggctttca
    23281 tcattgccta tcttgttgca atgatcatga ttatcaaaaa aagaaaagcg ctccggaaaa
    23341 atattgcggg ctgaatcttt ttgtctggag atatccctgc accgcaggag ttcataaagt
    23401 agaaagtcct caaacttata aagtagaaag acacttcact attttccgac ttcaaatcaa
    23461 aaatcaagag tcataaatcg tgaatcatcg tgtcttcacg taaaacgtga caagttgtgc
    23521 ccttgtggcg aaatttcttg cacaaatctg aatcatgctt catcgtagat gaagggtgat
    23581 tcaaatctgc ggaaatctgc ggacaaaagg gagttgtgct gcgaaggttt tcgaagagat
    23641 cccggcacgg tagcagaagc taaatcgcaa ccggtgaccg ggaactcaga gagtattgtt
    23701 tttgtgattt agagtcttcg aggcaaaatt aaaaacggcg cccggaaatc cggaacgccg
    23761 ctttattctg attctgtttt ttgaattagt gctttccgcc acagccagtg cccgagccgc
    23821 ttccgctgcc tttgcaacca ccggtttccg tcttgcctgc gccagagcat ccgctgccag
    23881 agccatgtcc ggggcaacat ccacccatgg tcttggcctg atagccgagt ttctgaatgc
    23941 gctcaacaat tacatcagcg ctggttttct ttgggctgaa ttttactgtc acaactttcg
    24001 tgtcaacatc tgccacggct tctttcactc ctttgactgt tgccagttcc gcttcgattt
    24061 tttctttgca ggaaccgcaa tgcagattgg tctgaatttt aatctcttca actttctttg
    24121 gtttttcctt cgtttcggtc tgaccaaaag taatcatcgg caatacaaac agggctgccg
    24181 caaccattgc aaaaatgcgt gttttcattt atcctccttt tttgtttaat ttttctttcc
    24241 tgaatccatg cgacctatcg cacagctgat gtaggatttc actacacggc ctgctgagtt
    24301 ttcacctacc ctcggatatc cgagcgtttt cagtttcctt gcaaactctt cggtacgatc
    24361 ggtctgcccc tcgtaaaaaa tggtgacttc cgatgattca tgattcacct gaacatcggc
    24421 cacgccatcc attgaagaaa tgctcttcgt gattgtattt gcacaaccat ggcatttcag
    24481 attttctatt tcaattattg ttttcattgt tagtcttttt tattgaattt gattttcacc
    24541 ccagcgtaaa ttttacgtcc cataacaggt ccccagataa cactgctgtc aaaataggaa
    24601 ctgtagggct tgtcagccga aataatcgga tgatgctgtt tgaaatccgt gacgttctct
    24661 gctcctgcat aaattgtcca atgcttgaaa ttgcgcgcca gctgcagatg tacaacaata
    24721 tgctccggcg aatattcatc gagctgatac ggaacaggaa actcactgcg atccgggagg
    24781 cgcgatttac caataaactg agccgttgca tcggcctgcc agcgtttcat gttgcttgca
    24841 aaggccagtg taaccagacc tttataattg cttacaagtg gcttctgctg caattctcca
    24901 tcgcgcaaag attgctgaac atcattgtac cgaaatgctg ttcgcacttc aagatgtctg
    24961 aataccggat acgaaatttc gatctgcgca ttgtgcgaat acgatttccc gttaaggttg
    25021 taaacataaa caaccgacgt atccgattcc atgtcaatca tcatctgatg ctgaaaatcg
    25081 gttttaaaat actcgaacga caccatcaat tccttgccca aaacgtcaaa atagcggaca
    25141 agagttgcac cataattcaa agcttcctca agctccggtt tattcagaaa tactattcct
    25201 cgcgacgaag tcagcaacga aatgttttcg ggaatcacat aaggagaacg gtatcccaat
    25261 ccagctgaag cacgaagtgt tgttttctca ttgaaatgat atttcatatg caagcgggga
    25321 gtggtgagaa aaccaaaacg attgttataa tcttcgcgca aaccagccat cactgtgatc
    25381 ttttcaggca gtgtgtacgt gtattgaaag aaaacacccg gcaccatttc ttcggtattc
    25441 catgaactgt cattgagctg ctccgacaca ttgtcgtaaa tgaaattgaa ccctgcatca
    25501 tactgatgct tcgtctcttt gaaatcggaa tggaaaataa aattcgaata gaacgatttc
    25561 tgaagtccgc tgtaggtctt gtttccaaac aacatgtcca aatcatgcac ggaaaaagaa
    25621 ttgataaaac cgaaactggt tcccggttta tttttaaaaa tataaccggt ctttacaaat
    25681 gcttctcctc ttctgttcga gatttcggat ttccagagtc cttccggaga aacaacaaaa
    25741 taagggctgc ctgtaatctg gcctccggag cgggtttcat cgagcagacg aacaccccat
    25801 cgcatttcga gtcccttcgg actgatgtat tgccagaaat tggcagcatt gatctggcga
    25861 ccccggttca tatcaacaaa accatccata ttgtggtcga tttcagaatt attgaacgaa
    25921 tagtgtccga gcaaaatggt gcttaccttt ttattcagat cggcagcagc attgatgttg
    25981 aattcatgcc ggcctgcatc attgagataa taattgaaga aaaatttctc cttggcttcc
    26041 ggctttttaa actcagcatt gatctgccct gtaatactct cgtagccgtt gattacagcc
    26101 gatgttcctt tcgaaatgga aatggagttc atccatgtgc ccggaatgta ttcaagccca
    26161 aatgctgatc caaggccgcg catgaaagga atattctcaa tcataagctg gctgtaaacg
    26221 ccgttcaggc caagcagctg aatctgtttt gcaccactga cggcgtcggt ataattcaca
    26281 tcgaccgaag cgtttgtttc aaaactttcc gataaattgc aacaggctgc acgctgcaat
    26341 tcaccactcc cgacttgctg ggtccacaag ggattcaatc tttcaagatg cgatgcagcg
    26401 ttggtttcaa gaatatcaac ctgttgcaga atattgcttt cgcgcaatac tatctcgata
    26461 tttccatcac tgaaatcaaa aacagtatca ctttcaaaac caacataaga cactataatc
    26521 ccaatgccgg ccggtggttc agcaatggaa aaactaccat ccatttcact gacaactcct
    26581 tcctgtttgt cagaccaata cacattggca gcaggcagat gcactttgtt accgctcgga
    26641 ctctcgccat ataccacacc ggagatctgt gcatacccgg acgtcagcaa catggtaaac
    26701 accatgactg acaaaaatat ttttatcatg caatattgat ttagttgtta cacatctgtt
    26761 tgatcaaaca gtgaaacaac aatcaatttt taatgcatga tgcttaataa ccgtggattt
    26821 acctgacaat tcaggtgggg aagaataatc gccggataaa taatcctttg taattttcat
    26881 ctccggatca acaaaaacaa tcttaaccgc aggattcagt aacgaagaaa gatttgaaat
    26941 ttccggacgt tcctctgatt gcgaaacatt atcaacagtg cgaataaaaa catgcacatt
    27001 ttcacaacac gaagtttctt tttcatcgtg gccgcaacaa gatgaattac aggtgcggga
    27061 atgctcagcg tactcatgtt cacaagactc aggtgactga aaagaaacca tcatttcacc
    27121 tgttgtgtgg cacatatgtt tgaaaatatt aaatcctccc gatgaaaaaa ccatcaatgg
    27181 gataaggacc aacacaaaac tattgcgcaa tattttcatg ccgcaaagtt acgaaacaaa
    27241 aacaaaaatc aactcgttca aacaatgttt cccggctgat atttatcagt ttcctgtttt
    27301 ttgataaatc tgcagatact tatgcacatt ctttgtcaaa tcatatccgt atgccgaccg
    27361 aaaacgcaaa tcatttttat caaaattttt ctcagcaata cgattgcaaa tattcatata
    27421 atcagtatcc cttgtgtatt ctgcaacaaa accaaaacca tccttttcta tatttccgag
    27481 atcaccaacg ccagcattgc aaataaccgg aacgcccatg gcaagcgact cgccaagctt
    27541 tatgggcgat gatgccattt tgctgaatgc cggtttaata aacaggatgc tataatcggc
    27601 cagcgaaaga accag
//
LOCUS       DAOPKE010000033        27595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_1837_length_27595_cov_13.846514, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000033 DAOPKE010000000
VERSION     DAOPKE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_27595_cov_13.846514"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKY63_06020"
     CDS             complement(<1..757)
                     /locus_tag="PKY63_06020"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPF00204.1"
                     /translation="MARQSTPQKATALDLSPGKKMYFASDLHLGIPASRDVERAFVQW
                     LNQIETDCQYLFLCGDLFDFWFEYRQVAPRGHVRMLGKLAQMADSGIQIYAFTGNHDM
                     WMFGYLEQEIGWKLFREPQRFLVNGKTIEIGHGDGLGPGDKKYKFIKRMFACRFNQWL
                     FARLHPNFSFGLARWLSRKSRIANGAADEKYNGDDKEFLVQYIQNTPAELRADIYVFG
                     HRHLPLDMKIGNAHYINLGEWVNHKTYGVFDGEM"
     gene            complement(712..1977)
                     /locus_tag="PKY63_06025"
     CDS             complement(712..1977)
                     /locus_tag="PKY63_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00205.1"
                     /translation="MRYTNFSGLYKTALILLLIVSVTLRGVEELTAQNLSFTPRLYTL
                     KDDSSLRETFVFPLVGWNENDHWMPGVYVTNQMTDKKKFRYQLMPMYSTDTKSLTGTG
                     RISINRYFENRSASTVSLFLQGKSFHLPSYEGDPMRYYQFETGIKTILETGSDSAPAQ
                     HTLELKYHQNGTEEMMWISNGTIYVPEQHHKILNAMRAAWTFEKYDSTQPFSTSVKAD
                     VWGDFARLSANYEYDYNYEPKRWFTFRAFAGVYLFHDSVFAQNHDARFSPNMPGWKTD
                     IFYDGWFIGRNVYDAFYRMQGMPGPGGLYSISPVGQSWSWTLSVSGSVSMPFDLPLRI
                     YAAAGISPDPLKANKPFGIFEGGLLITTPQKIFEIAFPLVHDKSSKEALLLNTDKYWQ
                     RIRFIINFDRINPHTWLDNPHHRKQLHLI"
     gene            complement(1946..>2575)
                     /locus_tag="PKY63_06030"
     CDS             complement(1946..>2575)
                     /locus_tag="PKY63_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPF00206.1"
                     /translation="ESLGSLFVLYQYFGRQPVLDPFEYFSVYENLAKKLAAKYPDFEH
                     VGHLNMKVNKTRLAIKEEEEIKNRLDTGKVAPDFSLPDINENQFKLSEFQGYVVLIHF
                     WASWSPESMRQLPAIRQYQQNYGPKGLAIVSISFDYDREMWTATVEKEKMKWTNICDL
                     KYTDSPIAKLYHIEKTPYFFLLDREGIIVSKNSNIDIVGNEIYKLFRPI"
     assembly_gap    2576..2675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2676..3297)
                     /locus_tag="PKY63_06035"
     CDS             complement(<2676..3297)
                     /locus_tag="PKY63_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HPF00207.1"
                     /translation="MKKLLFIFLSLTSFASATLLLSSCGNSDTASENEILIEGRFIHS
                     RANVVFIDVIHVEDIERTDSLILDEDGTFRFRRETDNPLFLKISTAEDNFITVIAEPG
                     QTITLSGDINALAESYTVSGSPASEILAQYLAFSREQFKKLDTLALVWENNKYAENKM
                     AVRDSLDSTAKIIYKNQTDYTLDLVNKNPESLGSLFVLYQYFGRQPV"
     gene            complement(3306..3782)
                     /gene="cdd"
                     /locus_tag="PKY63_06040"
     CDS             complement(3306..3782)
                     /gene="cdd"
                     /locus_tag="PKY63_06040"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPF00208.1"
                     /translation="MKKQELKISFTSFQNTDELNKKDQKLVEKAIEATKTAYAPYSEF
                     YVGAALQLANGKIIIGSNQENSAYPSGLCAERVALFQAGTLFPDIAIETLVITARTKG
                     KKLKTIANPCGACRQVMSESTMRQNKPFRIILIGENGQGYIFDSVDALLPFSFKFE"
     gene            complement(3757..5343)
                     /locus_tag="PKY63_06045"
     CDS             complement(3757..5343)
                     /locus_tag="PKY63_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPF00209.1"
                     /translation="MEVQKANRFHQWKYPVVIGLMLTGLVFSKWLLSVSQFFLLFAVL
                     SDKTSRKKLTEAFQSPLVLSLISIYVIHLAGMLWTSDMKYGFKDLQNKLPMLALPVLF
                     YAAGPLKKNTAIHLLLFFTFNVFLLSAVSLINYYLSPETTKHIVIGQSHIRFSLLICL
                     SIIILIHFSDLKYKWYIYTIIPLIAFLLFFLFILESFTGYVVLVFLLFALPLIYYDFI
                     RKRKWRFILPLIMLAVFTLALTGFLNVKNSCFPKPKESNFSTLPKQTINGNYYIHDTI
                     SLLSENGYYMYINLSVPEMIPAWNNRSKLKIDESDSTSAERINILTRYLTSKGLSKDS
                     AGVMQLSKKDIKNIEQGYSNYLLPDFDPYRKRIYRFLWELHYYNMTGDPSGHSLTQRF
                     EYWITAASIIQQHPAFGCGTGDINNAFKQQYQKNNSKLKKEYRLRSHNQFLSITVALG
                     FAGLLVFVFSLAAPFLFRKIPDYKLYLGFIFIFLLSLLNEDTLETQVGVTFYALFNSF
                     LLFILPNKNKSENEKARAEN"
     gene            complement(5347..6996)
                     /locus_tag="PKY63_06050"
     CDS             complement(5347..6996)
                     /locus_tag="PKY63_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPF00210.1"
                     /translation="MKKYLNKFIISLLIVVGIFSTSSTNSDFEISKNLDIFATLYREL
                     NQNYVDDLQPGELMKIAIDEMLKSLDPYTVFFPESDIEDVKFMTTGQYGGIGSLIQQQ
                     DNRIIITEPYEGFPAQINGLKAGDQILEINGKSMAGKTVSEVSDLLKGQPGSKVDLLI
                     KHPFDEKPETITIQRKEITISDIPYTGMVDTKVGYIKLRGFTMNAGNQVKKAFEDLKK
                     ENTEMEGLILDLRGNGGGLLNEAVNIANIFVDRGITVVQTKGKLSDSNKSYKTLNPAS
                     DKNIRLAILIDGYSASASEIVAGFIQDVDRGVIIGQKSFGKGLVQNVIPLSYNTSFKV
                     TTAKYYIPSGRCIQAIDYSKKINGKAVHIKDSAKQKFTTKNGRIVYDAGGILPDIDLK
                     PDSLSEITISLIIKNHVFNFVTQYVHDHASIPSPDKFEFSAADYQLFLSYLEGREYDY
                     KTSSERKLEELIEAMKEDKYYDHSTDEISRLQTKVGHNKQRDLELFSNEIINVIANEI
                     VSRYYYQKGRIIYDLRTDKEVKKAVEILRDPAQYNKILSGK"
     gene            complement(6977..7240)
                     /gene="yidD"
                     /locus_tag="PKY63_06055"
     CDS             complement(6977..7240)
                     /gene="yidD"
                     /locus_tag="PKY63_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPF00211.1"
                     /translation="MKRPIANFFVLFIRFYQVAISPWLPSSCRFTPTCSSYGIEALRK
                     HGFFRGGWLTIKRIVRCNPWGGSGYDPVPEKNYKSCKHEKVSE"
     gene            complement(7221..7613)
                     /locus_tag="PKY63_06060"
     CDS             complement(7221..7613)
                     /locus_tag="PKY63_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPF00212.1"
                     /translation="MKPFSLPRKERIKGTTQPSLLFHHGSLYNETPFLCRFIFTPADC
                     GGLQILFAVPKKRIKKAVDRNRLRRQMREAWRLNAGPLREKCINNSVLLQLGFYYQSN
                     DILPYSIIEQKIKSLIERLIQHHEKTDC"
     gene            complement(7619..8374)
                     /locus_tag="PKY63_06065"
     CDS             complement(7619..8374)
                     /locus_tag="PKY63_06065"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPF00213.1"
                     /translation="MVKIKNILVSQPKPAEVEKSPYQELISKHNVKIEFKKFFAVVGL
                     SSAEFRQSRINLAEFTGIIFTSRNAIDYYFSLAKDLRIEISENMKYFCTSDQIAFYLQ
                     KYIQFRKRKIFYGDSTYPSLLPLLKKHKDEKLVLPCNDSPNEELYSMLTANNVNFQKA
                     VIYKNESANLKDFDVKAFDLICFFSPSGIASLKENFPKYTQGEQLIATYGKTTLQAAE
                     CSKLRVDIPAPTATAPSMTMAIDEFIKASKKKK"
     gene            complement(8398..9264)
                     /locus_tag="PKY63_06070"
     CDS             complement(8398..9264)
                     /locus_tag="PKY63_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPF00214.1"
                     /translation="MKIQPQTFKPFATEDTSSALYFHGRSMDESPVLFSKKQDSSIIR
                     QVKPIEYKTNSHFFSEVGNPESGRQSKQKPDNDFLFITALIVPIIITLWIFKSVFPRI
                     QGAFRAAFNNRFASIFMRNFSISNHLSTYIILLNTAISSSVGFWIFLLQKDIITDENN
                     TQALLMLLSGIIGYYLYRYIVIYLAKVIFMTHEATEHYLYRDYYINAIISGILPFLIF
                     AAAFSPATDIIWPVIVAIIALLQAYRLLLAIYTGILERTYGLFYFILYFCTVEIVPVA
                     IILKLIAERISQ"
     gene            9761..10963
                     /locus_tag="PKY63_06075"
     CDS             9761..10963
                     /locus_tag="PKY63_06075"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPF00215.1"
                     /translation="MKIIVLNCGSSSIKYQLFDMDKNEQVLAKGLLERIGIPNSLLKH
                     SSTGKDSFEEIRDIPDHTVGIQWIIDTLLDPVKGCLKDKTDINAAGHRVAHGGEFFSE
                     SAFIDSKTKAAIEKLIELAPLHNPANLKGILAIEALLPGLPQVAVFDTSFHQSMPEHA
                     FLYGLPYEMYKDKKIRRYGFHGTSHKYVAEKAATILGMDWTKMRIITCHLGNGSSIAA
                     ILNGKSIDSSMGYTPVEGLLMGTRVGDLDLGAMLAIMDREKMSNADANALINKKSGLL
                     GISGISSDMRDIEKAAAEGNSQANVALDVFAYRVKKYIGSYMAALNGLDLLIFTGGIG
                     ENDCDSRRRICSNLEGLGIEFDVEKNKGLRGKDAVISTENSKVKVMTVTTNEELVIAR
                     DTIRIVTK"
     gene            complement(11074..11949)
                     /gene="atpG"
                     /locus_tag="PKY63_06080"
     CDS             complement(11074..11949)
                     /gene="atpG"
                     /locus_tag="PKY63_06080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPF00216.1"
                     /translation="MANLKEVRNRIQSVTTTKQITSAMKLVSASKLRKAQDNILRFRP
                     YASKLREIMGRVAAGVDNLPTDGVFAQRNPDKILMVVFSANRGLCGVFNANIVKQINH
                     LIEGEYRKSFEAGKIDFISFGRKAPDLLKKKGCRFSKDYNHLIDKPSFEEVSGICDNI
                     VKEYIHAKYDAIVLVYNQFRNAAQQVPVSEMLLPFKLESGKQIAGNDYILEPGREKMF
                     TIIVPKTLKLQLYKALLDSIASEHGARMTSMHKATDNATEILKDLRLSYNKARQAAIT
                     NEIIEIVSGAEALKG"
     gene            complement(11961..13541)
                     /gene="atpA"
                     /locus_tag="PKY63_06085"
     CDS             complement(11961..13541)
                     /gene="atpA"
                     /locus_tag="PKY63_06085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPF00217.1"
                     /translation="MSEIKPSEVTAILRQQISGMNLGSSLEETGTVLEIGDGIARVYG
                     LSNARAGEMVEFEKTGLKGIVLNLEADNVGIVLLGNTKNLNEGDVVKRTGTIASIQVG
                     EGLLGRVINTIGEPIDGKGSIQGELYEMPLERKAPGVIFRQPVTEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQRENYEKGKPVFCIYVAVGQKGSTVAQIAK
                     TLEEKGAMPYTVIISATASMPAALQFYAPYAGAAIGEYFRDTGRPALAIYDDLSKQAV
                     AYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVIKSDEVAKQMNDLPESIRHLVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETNLFNSGIRPAINVGISVSRV
                     GGNAQIKAMKKIAGTLKIDQAQFRELEAFSKFGSDLDAATKAVLEKGTRNVEILKQPQ
                     YSPMPVEQQVAIIFAGTRGLLKSVPVKQVKNFESEFLHHLEDNHADLLDELRKGALSD
                     EMIAKLDKIALDIAGRYH"
     gene            complement(13554..14111)
                     /gene="atpH"
                     /locus_tag="PKY63_06090"
     CDS             complement(13554..14111)
                     /gene="atpH"
                     /locus_tag="PKY63_06090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPF00218.1"
                     /translation="MNNSQIPKRYARALFELADEINVLPATELDMRSLKKICDEAPEF
                     KQFLKSPIIKPALKKKVISSLFEQHFHELSMRFLKLVIHQGRETFMNEIAKEFMILCR
                     EKEGVVEVDLTAATKLNDSIVKQIEAKIASLTGLKPSITQKTNPSLLGGFVVSFGDNM
                     YDASLKKKISKISRDFRTNIYEKGF"
     gene            complement(14120..14611)
                     /gene="atpF"
                     /locus_tag="PKY63_06095"
     CDS             complement(14120..14611)
                     /gene="atpF"
                     /locus_tag="PKY63_06095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPF00219.1"
                     /translation="MLTTPGLGLIFWTSIVFLLLVWLLSKFAWKPIVAAIKKRNEAID
                     NALMAAEKARQEMTQLQVNNEQLLKEAQKERDIILAEARKIRDKIVEEAGVKAKEETE
                     RIIAAAHENIHFEKMAAITELKNQVAVLSLEIAEKIIQENLSGDAKQQELAQKMAGDI
                     SFN"
     gene            complement(14683..14883)
                     /gene="atpE"
                     /locus_tag="PKY63_06100"
     CDS             complement(14683..14883)
                     /gene="atpE"
                     /locus_tag="PKY63_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPF00220.1"
                     /translation="MVLAGIGAGLAVIGAGIGIGRIGGSAMDAIARQPEATGKIQTAM
                     IIAAALVEGVALFAVVVALIAK"
     gene            complement(14917..15999)
                     /gene="atpB"
                     /locus_tag="PKY63_06105"
     CDS             complement(14917..15999)
                     /gene="atpB"
                     /locus_tag="PKY63_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPF00221.1"
                     /translation="MQKLIFIFTLVFAGFSGLSAYAGSDHGHDKNKTEQNEGIDITQV
                     IFDHILDAHDWHVMTLNEGETNEKHVVVPLPVILWDEGRLVFFMSSRLHHAEHSEYQL
                     GTKDNFNGEYEGKIVRVNHDGQVASVPLDFSITKNVAALIFSASLLLLIFIPVARSYK
                     KAGNAAPKGLQGFMEPLILFVVDDIVKPNIGEKRYERYVPYLLTLFFFILINNLMGLI
                     PFFPFGANLTGNLAVTLTLAFFTLIIVNVSGNKGYWKHVFAAPGVPVWLLPIMIPVEL
                     IGIISKPFALMVRLFANITAGHIIVLSLISLIFIFQNLGIAPVSVVFVLFMDVLELLV
                     AALQAYIFTLLTSLFIGLAVQEAEHH"
     gene            complement(16162..16812)
                     /locus_tag="PKY63_06110"
     CDS             complement(16162..16812)
                     /locus_tag="PKY63_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00222.1"
                     /translation="MARLNHRKKKPLVPIIIFVVGMVLFVWVKLYNQKNIASAEIDTR
                     LKSAAGSLEMIVNDSMIDNAQQKIPVNFVDHEYIRVLANKIAKIHDVIYVYVMIESED
                     SALFVFSSYIESDITGDIVTDYLDYYYEATPTMMNAFSSNDPEIYDSSQDQWGNFRSI
                     YLPKKTRTGTPYLLCADVNMSEVVDYQLRYMVEFALSAVFLFLISLPLFIKMRQTK"
     gene            complement(16929..17549)
                     /locus_tag="PKY63_06115"
     CDS             complement(16929..17549)
                     /locus_tag="PKY63_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00223.1"
                     /translation="MGILLLLAIKLFIHERIITNNINFRLESSAGTIELVINDSLIEA
                     AATGKQPDKDRYSNMQKKIDRIASIHEVDYVYVVIENRDSVYFVICNILPSDIINNIE
                     IKYLDVFAEAPPELKKAFRMSDNIIFGKNSNKWGTFRSAYIPRKTKGGINYLLCSDKF
                     IRDINKELFRVLTEFLISFIYIILISFPLIYVQVNAIHSKKFKQKP"
     gene            complement(17755..18174)
                     /locus_tag="PKY63_06120"
     CDS             complement(17755..18174)
                     /locus_tag="PKY63_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00224.1"
                     /translation="MAKMLWEYEVKILLALEQAIGGNSEFLQFLLENGFPELAAFASA
                     VRADKKALKWLFDNGSPELGILSDAIDSEDAAIAWLKKNNLTFYIIFADACRGVTPAK
                     ELLKKNNMDVFLRLAITTQEVLKQQQFDAWDYHKFKK"
     gene            complement(18179..18757)
                     /locus_tag="PKY63_06125"
     CDS             complement(18179..18757)
                     /locus_tag="PKY63_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPF00225.1"
                     /translation="MNTGSDKISKVIVAITGASGAVYGLELIKQLVALECIKDIAVIY
                     SENGSKVWDFEKVMDIPVSKKITVFENNDMFACPASGSAGYQAMFIAPCSMGTLASVA
                     AGTAGTLILRAADVLLKERRPLILLAREAPLSLIHIKNMERVTRAGAIVFPASPFFYH
                     HPKDLREAISALVSRLISITGISTPQIEWGKE"
     gene            complement(18741..19343)
                     /locus_tag="PKY63_06130"
     CDS             complement(18741..19343)
                     /locus_tag="PKY63_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF00226.1"
                     /translation="MKKILFLFLLILTLQSSAQSFRGGFRAGLTGSQVDGDNLSGFDK
                     AGLTGGFMVAIPLNEFSDLAMEMLFVQKGSRRNPTKDNNLTKYVMRLNYIEVPVLFRR
                     QLKSNFGFETGLSFGILLDNENREFDINGAIPARPDFEKYELAAHLGFRYFFNEKQSI
                     NLRYSNSVLPIRKAPGISTYNYFIRGQYNMLLGLTYEHWF"
     gene            complement(19641..20009)
                     /locus_tag="PKY63_06135"
     CDS             complement(19641..20009)
                     /locus_tag="PKY63_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPF00227.1"
                     /translation="MNKSTSKSETGRFGEDEAALFLQSKNYEILHRNWRCGKDELDIV
                     ALHEEILVIIEVKTRTGTAFGEPWESVDDRKQQAIERAALAYLEEFSIDREMRFDIIS
                     IIVSPDKDVWIDHIENAFIP"
     gene            complement(20022..21932)
                     /locus_tag="PKY63_06140"
     CDS             complement(20022..21932)
                     /locus_tag="PKY63_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPF00228.1"
                     /translation="MHKLFFAISLAFLALNTHAQIFNINATKQIALDDAFKASRMLDV
                     PEKFVGQEYDLKYHRFNWNVDPDTCYISGSVTSYFIPVQNNLTQIDFSLKVNMMVDSV
                     KYHGAPAMSVHVNNEVNITGFPPLSAGILDSITVYYHGTPQATSGFISFVASTHGSGT
                     PALWTLSEPYGAYEWWPCKNDLSDKIDSIDIYVTHPNGYHAASNGILVSETVGATTTA
                     HWKHRYPIAAYLIAIAVSDYSIFSDDFSLSTGTIPMLSYVYPESLTDAQNMTPLLEDV
                     MQFYDSLIAPYPYMTEKYGHAQFGWGGGMEHQTMSFMGGFGYELMAHELLHQWFGDAV
                     TCGSWHDIWLNEGFATYFTALNYERTSPDYYWIIWKNNTVNHVTSDPGGSVFVYDTSD
                     VSRVFDSRLSYNKAAYMLHMLRWVVGDDNFFQGNRNYFNDANFYFGYAKSPDYIAHME
                     AQSGMDLTGFFDDWLYHEGYPSYTIVAMPIPGDSLSIAIDQTQSHSSVSFFEMPVPIQ
                     IDDSIYVFDNTFSGQEFRIPYTSFPTTMQFDPEFQLLSGFNQVILLSTQEHDVPEISL
                     YPNPAENQLFISGAENQPINCHIYDSRGALVISDEISANESVDIQRLATGAYRISVST
                     ADWQYSSSFIKK"
     gene            complement(22070..23701)
                     /gene="groL"
                     /locus_tag="PKY63_06145"
     CDS             complement(22070..23701)
                     /gene="groL"
                     /locus_tag="PKY63_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694639.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPF00229.1"
                     /translation="MAKQILYGVEARELLKKGVDALSNAVKVTLGPKGRNVIIEKSYG
                     APAITKDGVTVAKEIELPEKVNNMGAQMVKEVASKTNDAAGDGTTTATVLAQAIFNTG
                     LKNVTAGANPMDLKRGIDKAVAAVIENLKKQSKEIGDSFEKIEQVATISANNDNEIGK
                     QIAEAMKKVKKEGVITIEEAKGTETTVDVVEGMQFDRGYLSPYFVTDTEKMLTSFDNA
                     FMLIYDKKISSMKELLPILEKVVQTGRPMLIISEDIEGEALATLVVNKLRGSLRVAAV
                     KAPGFGDRRKEMLEDIAVLTGGIVISEEKGYKLEDADLSYLGQAEKVTVDKENTTIVK
                     GLGSKENIDARVAQIKAQMEATTSDYDREKLQERLAKLAGGVAVIKVGAASEVEMKEK
                     KDRFDDALHATRAAVEEGIVPGGGVAYIRAIEAIAGLTGGNEDEKTGIDIIRRAIEEP
                     MRMIVENAGLEGSVIVQKVKEGKADYGFNARTEVYENLLETGVIDPTKVTRVALENAA
                     SIAGMLLTTETVIVEIKEDKPAMPPMPGGGMGDMY"
     gene            complement(23730..23999)
                     /locus_tag="PKY63_06150"
     CDS             complement(23730..23999)
                     /locus_tag="PKY63_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPF00230.1"
                     /translation="MNIKPLADRVVIEPAQAETKTSSGIIIPDTAKEKPQRGTIVAAG
                     PGKKDEPVTVKVGDVVLYGKYAGTEITIDGKDYLIMKESDVLAII"
     gene            complement(24207..24542)
                     /gene="secG"
                     /locus_tag="PKY63_06155"
     CDS             complement(24207..24542)
                     /gene="secG"
                     /locus_tag="PKY63_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPF00231.1"
                     /translation="MGFYIFLVILMIIICVLLGLIVLVQNSKGGGLVAGMQSSNQFMG
                     VRQTADFLEKSTWTLAIIILALSLLSIFVIPRGQQQTEESRVKNYIESNELAPINTPQ
                     GEQPATPVE"
     gene            complement(24562..25284)
                     /locus_tag="PKY63_06160"
     CDS             complement(24562..25284)
                     /locus_tag="PKY63_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007368327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00232.1"
                     /translation="MPEAVIEKEIIEPVVIPEPEEIVESLIETQVAEETIVNISENNP
                     VISETDVVSEPVQEEPEAVVQEMEKTAVEVAEPQKEQDENTLEVQIQDEVSATMLNPE
                     EVKEEMNEKNQNSTVEEGRDPLKILQQRLAELNITKNEEPLDQEKQPEMEKETTEIIE
                     QFIKTEPTIKIDLNRLPDRRNLAEESTVEKFDVVSETLASIYEKQGRYEKALRMYEKL
                     LLANPEKSSYFAPLIENLKKKL"
     assembly_gap    25334..25433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25434..25694)
                     /locus_tag="PKY63_06165"
     CDS             complement(<25434..25694)
                     /locus_tag="PKY63_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00233.1"
                     /translation="MSISDFHITTADAARILEQHPYSAHLETIVLSQLKKENPELFAE
                     RLKQSCFKVPDRYILYVNLHETIADNSYSAPEPEEYVAPDPIP"
     gene            complement(25704..26207)
                     /locus_tag="PKY63_06170"
     CDS             complement(25704..26207)
                     /locus_tag="PKY63_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPF00234.1"
                     /translation="MIRRFTIGFLLLPVFLSSCLRVTFTGASIHPEAKTFSVQYFENN
                     ATLINPTLAKTMTEKLQDKIMGQTRLTMVNQNGDLAFEGEITGYTLQPVAIQGNETSQ
                     LTQLTVNINVRFFNRLDESKNFESRFSRFQQFSSSSSLASVEDGLIETITEELVDDIF
                     NKALVNW"
     gene            complement(26204..27445)
                     /locus_tag="PKY63_06175"
     CDS             complement(26204..27445)
                     /locus_tag="PKY63_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPF00235.1"
                     /translation="MNEITSIKQRFGIIGQSPLIDRAIDVARQVAPTDLTVLITGESG
                     TGKEVFPKIIHQFSARKHGPFIAVNCGAIPEGTIDSELFGHEKGSFTGATDARKGYFE
                     VVNGGTIFLDEVAELPLSTQVRLLRVLESGEFLRVGSSKVIKTDVRVVAATNVKLGEA
                     IANGKFREDLFYRLNTVPIHIPPLRERKDDIVLLFRKFAADFAEKYRMPALILTEEAQ
                     NMLRQNYWSGNVRQLKNVTEQISIIEQEREVTPNTLAKYISDSNPTMLPVLKRHFENS
                     DEHANERELLYKVLFDMKKDMAEMKKLIAEIISATGIDPTMPGRTIPAGESIPYQPEF
                     YKPQYSTPPQDFQHPREAVNEIPIDYSEEIEESLSLEKKEKEIIAKALEKHHGRRKNA
                     ATELGISERTLYRKIKEYGIE"
BASE COUNT         7333 a         5544 c         6132 g         8386 t
ORIGIN      
        1 tcatctctcc atcaaaaaca ccatatgttt tgtggttgac ccattcgccc aggttgatgt
       61 agtgcgcgtt tccgattttc atatccagcg gcagatggcg gtggccaaac acataaatat
      121 cggcacgcag ctctgcgggc gtgttttgaa tatactgtac cagaaattcc ttatcgtcgc
      181 cgttgtattt ttcatcggca gcgccattgg caatgcggct tttgcgtgac agccagcggg
      241 ccagcccgaa agaaaaattc ggatgcagcc gtgcaaacaa ccattgattg aagcgacagg
      301 caaacatgcg tttgataaat ttatattttt tatcgccggg tccaagacca tcgccatgac
      361 caatttcaat tgtttttccg ttcaccaaaa aacgctgtgg ctcgcggaac aatttccatc
      421 cgatttcctg ttcaagataa ccaaacatcc acatgtcgtg gttgccggtg aatgcataaa
      481 tctgtatgcc ggaatcggcc atctgagcca atttgccgag catacgcaca tgcccgcgcg
      541 gagccacttg gcggtattcg aaccaaaagt cgaacagatc gccgcagaga aataaatact
      601 gacaatcggt ttcaatctga ttcagccact gcacgaaagc ccgctccaca tcgcggctgg
      661 cgggaatgcc gagatgaagg tccgaagcaa aatacatttt tttgccggga ctcagatcaa
      721 gtgcagttgc tttctgtggt gtggattgtc gagccatgta tgcggattga tgcggtcaaa
      781 gttaataata aatcggattc tctgccagta cttgtcggta ttgagcaaca gcgcttcttt
      841 gctgcttttg tcgtgcacga gcgggaaagc gatttcgaaa attttctgtg gtgtggtaat
      901 gagcaagccg ccctcgaaaa ttccaaaagg tttgttggct ttcagtggat caggcgaaat
      961 tcctgctgca gcataaatgc ggagcggtaa atcgaaaggc atggatacgc tgccggatac
     1021 cgacagtgtc cagctccagc tttgtccaac gggcgaaatg ctgtacagtc cgccgggccc
     1081 gggcatgccc tgcatgcggt aaaaagcatc atacacattg cggccgatga accagccatc
     1141 gtaaaaaata tcggttttcc agccaggcat attggggctg aagcgggcat cgtggttttg
     1201 ggcaaaaacg gaatcgtgga aaagataaac acctgcaaaa gcgcgaaacg tgaaccagcg
     1261 cttcggttcg tagttgtagt cgtattcgta attggcactc agacgggcaa aatcgcccca
     1321 cacatcggcc ttaaccgaag tgctgaatgg ctgcgtggaa tcatactttt caaatgtcca
     1381 tgcagcgcgc attgcattca gaattttgtg atgttgttcg ggaacgtata ttgttccgtt
     1441 gctgatccac atcatttctt cggtaccgtt ctgatgatat ttcagctcga gtgtgtgttg
     1501 cgcaggagcg ctgtcggagc cggtttccag aattgtcttt attcctgttt cgaactgata
     1561 atatcgcatt ggatcgcctt cataggaagg cagatgaaaa ctttttccct gcaaaaacaa
     1621 tgaaactgtg cttgctgagc ggttttcgaa gtagcgattg attgaaattc ttccggttcc
     1681 ggtcagtgat tttgtatcgg tgctgtacat gggcatcagc tgatagcgga atttcttttt
     1741 gtcggtcatt tggtttgtaa cgtacacacc cggcatccag tggtcgtttt cgttccatcc
     1801 caccaaagga aaaacaaatg tttcacgaag cgatgaatca tctttcaacg tatataatcg
     1861 tggagtaaaa gacagatttt gtgctgtcaa ctcttcgact cctctcagag tgacagaaac
     1921 aatcaaaagc agtatcagag ccgttttata taggcctgaa aagtttgtat atctcatttc
     1981 cgacaatgtc gatgtttgag tttttactga caattattcc ttcacggtcg agcaggaaaa
     2041 aatagggcgt tttttcgatg tgatagagtt ttgcgatagg agagtctgtg tatttcagat
     2101 cgcagatatt ggtccatttc attttttcct tttcaacggt ggcagtccac atttcgcggt
     2161 cataatcaaa cgatatggaa acgatggcca gccctttcgg tccgtagttt tgttgatatt
     2221 gtctgatggc gggcaactga cgcatacttt cgggtgacca tgatgcccag aaatggatca
     2281 gaacgacata tccctgaaat tcggatagtt tgaactgatt ttcatttata tcgggcaatg
     2341 aaaaatcagg tgccaccttt ccggtatcga gccggttttt gatttcctct tcctctttaa
     2401 tggccagccg tgttttattg actttcatat tgaggtgccc gacgtgttca aaatcaggat
     2461 attttgcagc cagttttttc gccagatttt catacaccga aaaatattcg aatggatcaa
     2521 gcacaggctg gcggccgaaa tactgataca ggacaaaaag acttccgagt gattcnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcaca ggctggcggc cgaaatactg
     2701 atacaggaca aaaagacttc cgagtgattc cgggttttta tttacaagat caagtgtgta
     2761 atccgtttga tttttataaa tgattttagc cgtcgaatcg agcgaatcgc gcacggccat
     2821 tttgttttca gcgtatttat tattttccca gaccagtgca agtgtatcga gttttttaaa
     2881 ttgttcgcgt gaaaacgcaa ggtactgtgc gagaatttca gatgccggag atccgctcac
     2941 agtgtatgat tcagccaatg cattgatgtc gccgctgagt gtaatggtct gtccgggttc
     3001 ggcaataaca gtaatgaagt tatcttcggc cgttgatatt ttcaaaaaca gaggattgtc
     3061 ggtttcgcgc cggaagcgga aagtgccgtc ttcgtcgaga atgagggaat ccgttctttc
     3121 aatatcttcg acatggatga catcgatgaa aacaacattg gcacggctgt gaatgaaacg
     3181 accttcgatc aggatttcgt tttcagatgc tgtgtcgctg ttgccgcagg aactcagtaa
     3241 tagtgttgcc gaggcaaaag acgtaagaga aagaaaaatg aaaagcagtt ttttcatgag
     3301 tcggatcatt caaatttgaa actaaaaggg agtaaggcat caacgctgtc gaaaatatat
     3361 ccttgtccgt tttcgccgat aagaattatt ctgaaaggtt tgttttgtcg catggtggat
     3421 tcgctcatta cttgtctgca ggcaccgcat gggttggcta tggttttcag ttttttgcct
     3481 ttggtgcggg ctgtaatgac caaggtttca atggcaatat ccgggaacaa tgttccggct
     3541 tgaaaaaggg caacgcgttc ggcacacaga ccacttggat aagccgagtt ttcctgattg
     3601 cttccgatta taatttttcc gttggccagt tgcaatgccg ctccaacata aaattcggaa
     3661 tagggtgcat aagctgtttt ggtagcttcg atggcttttt caacaagttt ctgatccttt
     3721 ttgttgagtt catcggtgtt ttgaaaggat gtaaaactaa ttttcagctc ttgctttttc
     3781 attttcggat ttgtttttat tgggtaatat gaaaagaaga aaactattga ataatgcata
     3841 aaaagttaca ccaacctgag tttcgagtgt gtcttcatta agcagagaca acaaaaatat
     3901 gaaaataaat ccaagatata acttataatc cggtattttg cgaaacagaa atggagcagc
     3961 caaggagaaa acgaaaacga gaagtccggc aaaacccagt gctacagtga tagacaggaa
     4021 ctgattatgc gatcggagac gatattcttt ttttagtttt gaattgtttt tttgatattg
     4081 ttgtttgaaa gcgttgttta tatcacctgt tccgcagcca aatgcaggat gctgctgaat
     4141 gatggatgct gctgttatcc agtattcaaa tcgttgtgtc agtgaatgcc cgctcgggtc
     4201 gccggtcatg ttgtaataat gcagttccca gagaaaacgg tagattcgtt ttcggtaggg
     4261 gtcaaaatcc ggaagaagat agtttgagta accttgttct atgtttttga tgtctttttt
     4321 gcttagctgc atgactccgg cactgtcttt tgataaaccc ttgctggtca aatatctggt
     4381 gaggatattg attctttcgg ctgatgtgga atccgactca tcaattttta gtttactgcg
     4441 gttgttccat gcgggtatca tttctggaac tgagaggttg atatacatgt agtacccgtt
     4501 ttcgctcaac agactgatgg tgtcgtggat gtaataattt ccattgatgg tctgtttggg
     4561 taatgtggag aaatttgatt ctttcggttt tgggaagcaa ctgtttttga catttaaaaa
     4621 accggtaagt gccagtgtga aaactgcaag cattataagc ggcaaaatga agcgccattt
     4681 tctttttcgg ataaaatcat aatatataag cggcaaggcg aaaagcagaa aaacgagaac
     4741 aacataccct gtaaaagatt caagaataaa aagaaaaaac agtaaaaacg caataagcgg
     4801 tattattgta taaatgtacc atttgtattt caggtcgctg aagtggatca gaatgattat
     4861 tgaaagacaa atgagaagcg aaaaccggat atggctctga ccaataacaa tatgtttcgt
     4921 tgtttctggc gacaggtaat aatttatcaa actgactgct gaaagtaaaa aaacattgaa
     4981 agtaaaaaac agcagaagat gaatggctgt gttttttttc agcggtccgg ccgcatagaa
     5041 taaaacaggc agagccagca ttgggagctt gttttgaagg tctttaaatc cgtatttcat
     5101 gtcgcttgtc cataacattc ctgccaaatg tatgacatag attgaaatga gagacagaac
     5161 caaaggagac tgaaaagctt ctgtgagttt ttttctcgat gttttatcgc ttaaaaccgc
     5221 aaaaagaaga aaaaactgag atacggaaag gagccattta gaaaacacca aacccgttaa
     5281 catcaggcca atgacaaccg gatatttcca ttggtgaaat ctgttggctt tttgtacttc
     5341 catttgttat ttgcctgaaa gaattttgtt gtattgtgca gggtctctca gaatttcaac
     5401 ggctttcttt acttctttat ccgtacgcaa atcatatatg attcgtcctt tttgatagta
     5461 atatcgcgaa acgatttcat tggcaataac attgatgatt tcattgctga acaattccag
     5521 atcgcgttgt ttgttgtgtc ccactttggt ttgcagccgg ctgatttcgt cggttgagtg
     5581 gtcgtagtac ttgtcttctt tcatggcttc aataagttct tcgagtttgc gttcgctcga
     5641 tgttttgtaa tcatattcgc gaccttcgag atagctgaga aacagttgat aatctgctgc
     5701 ggaaaactca aatttatcgg gtgaaggaat acttgcgtga tcgtgaacat attgagtgac
     5761 aaaattgaaa acatggtttt ttatgatcag actaatggtg atttcgctca gcgaatctgg
     5821 cttcagatca atatctggca agatgccgcc ggcatcatac actatccggc catttttggt
     5881 agtgaatttt tgtttggctg aatctttaat atgaacggct ttaccattga tttttttcga
     5941 gtagtcgatg gcttgaatgc aacgtccgct tgggatgtag tatttagcag tagtaacttt
     6001 gaaacttgtg ttgtaactga gtggaattac attctgaaca agaccttttc caaaagattt
     6061 ttggccgatg ataacaccgc ggtcaacatc ctgaataaaa cctgcgacga tttcgctggc
     6121 cgaagcgctg taaccatcaa tgagaatggc cagacggatg tttttatcgc tggcagggtt
     6181 gagtgttttg tatgatttgt tgctgtcgct gagtttgcct ttggtctgaa caacagtaat
     6241 tcctctgtca acaaaaatat ttgcaatgtt aacggcttca ttgagcaatc caccgccatt
     6301 tccgcgcaga tcgaggatga gaccttccat ttctgtattt tcctttttca ggtcttcaaa
     6361 tgcttttttc acctgatttc cggcattcat tgtaaatcca cgtagtttga tatatcccac
     6421 tttagtatcc accattcctg tgtagggaat atcgctgatg gtaatttctt tgcgctgaat
     6481 ggtgatggtt tcaggttttt cgtcgaaagg atgtttgatt aaaagatcga ccttacttcc
     6541 aggttgtcct tttaaaagat cgctgacttc actgacggtt ttaccagcca tggatttgcc
     6601 attgatttca agaatctggt cgccggcttt taatccattt atctgagcag ggaacccctc
     6661 gtagggttct gtaatgatga tgcggttatc ctgttgttga atcagtgaac cgatgccacc
     6721 atactggcct gtagtcataa atttcacatc ttcgatatca gattcgggaa aaaagacagt
     6781 ataagggtcg agcgatttaa gcatttcgtc gatggcaatt ttcatcagtt caccaggttg
     6841 cagatcatcg acatagtttt gattcagttc gcgatagagt gtggcgaata tgtcgaggtt
     6901 tttggagatt tcgaaatcac tgttggttga tgaggtcgaa aagattccga caacaatgag
     6961 aagcgaaatg ataaatttat tcagatactt tttcatgttt gcaggatttg taattttttt
     7021 caggaaccgg gtcatagccg ctgccacccc agggattgca tcggacaatt cgttttatgg
     7081 tgagccagcc gccccggaaa aaaccatgtt tacggagagc ttctatgcca taggatgagc
     7141 atgttggagt gaaacgacat gatgatggaa gccaaggcga aatggctacc tgatagaatc
     7201 ggataaacaa cacaaaaaaa ttagcaatcg gtcttttcat ggtgttgtat taaacgttca
     7261 atgagtgatt ttattttttg ttctatgatt gagtagggaa gaatgtcgtt gctttgataa
     7321 taaaaaccaa gttgtagtaa aacggaattg tttatgcatt tttcgcgcag cggtccggca
     7381 ttgagtctcc aagcttcgcg catttgtctt cgcagacggt ttcggtcaac agctttttta
     7441 attctttttt ttggaactgc aaataaaatc tgtaatccac cgcaatcggc cggagtgaag
     7501 ataaaccggc ataaaaaggg tgtttcattg tataaagaac cgtggtgaaa cagcagtgaa
     7561 ggttgggtgg ttccttttat ccgttctttg cggggtaagg aaaatggttt cacaatgact
     7621 attttttctt ctttgaggcc ttgatgaatt catcaatggc catggtcatg ctgggcgctg
     7681 ttgcagtagg agccggaata tcgacacgta atttagagca ttcggcagct tgcaatgtcg
     7741 tttttccgta ggtggcaatc agttgctccc cctgagtgta tttgggaaag ttttctttta
     7801 aagaagcaat gccacttggg ctgaaaaaac agataagatc aaatgctttt acatcaaaat
     7861 ccttgagatt ggcactttcg tttttgtaaa taaccgcttt ctgaaaattt acattgttgg
     7921 ccgtcagcat tgaatataat tcttcattgg gtgaatcatt gcatggaaga accagctttt
     7981 catccttgtg cttttttagt aatggcaaca gactgggata ggtcgaatca ccataaaata
     8041 tttttcgttt gcgaaactga atatattttt gaagataaaa ggcaatctga tcagatgtgc
     8101 agaagtattt catgttttct gaaatttcaa tccgcaggtc tttggccaga gaaaaataat
     8161 aatcaatggc attgcggctt gtaaagataa ttccagtaaa ctctgccaga tttattcgcg
     8221 attgacggaa ttcggctgaa ctgagtccta caactgcaaa aaatttctta aattcaattt
     8281 ttacattgtg cttcgaaatc aattcctgat acggggactt ttcaacttca gccggcttgg
     8341 gttgagatac cagaatgttt ttaattttca ccacgttcgc gagttttgag attgaaatta
     8401 ttgtgaaatg cgctctgcaa ttagttttaa gattatcgca acaggtacga tttcgacggt
     8461 gcaaaaatac aaaataaaat aaaacaatcc gtaggttctt tccaaaattc cggtgtagat
     8521 ggccaacaac aatctgtagg cctgtaacag tgcgattatt gcaactatta caggccatat
     8581 gatatctgtg gctgggctga atgctgctgc aaaaatgaga aatggcagta tgcctgatat
     8641 tattgcatta atataataat ctctgtacag ataatgttct gtcgcttcat gagtcataaa
     8701 aatgactttt gccaggtaaa tgacaatata gcggtataaa taatagccaa tgattccgga
     8761 aagcagcatt aaaagtgcct gtgtgttgtt ttcatcagta atgatgtctt tttgcaataa
     8821 aaatatccag aatccaacac tgctactgat tgctgtgttt aacagtatga tgtaggttga
     8881 taaatgattg ctgatggaaa aattacgcat gaatatcgat gcaaagcggt tgttgaatgc
     8941 agctctgaaa gctccctgaa tacgcgggaa aaccgattta aaaatccaga gtgtaataat
     9001 gatgggaaca attaatgctg ttatgaacaa aaaatcattg tctggctttt gtttgctttg
     9061 ccttccggat tccggattcc caacttctga gaaaaaatga ctgtttgttt tgtattctat
     9121 aggtttaacc tgacgtatta ttgaagaatc ttgttttttg gaaaataaaa ccgggctttc
     9181 gtccatgctg cgcccatgaa aatacagcgc cgaagaggtg tcttcggttg caaatggctt
     9241 gaatgtttgc ggctggattt tcaatgattt ttgttttccg gtgcaaagat acataagaac
     9301 atcgaacact gaatatcgag cactgaacac tgaacatcga acatcgaata tcgaacaccg
     9361 aacactgaac atcgaacatc gtacactgaa catcgaacat cgtacactga acatcgaaca
     9421 tcgaacactg aatatcgagc actgaacact gaacatcgaa cactgaacac cgaacactga
     9481 acatcgaaca ctgaacatcg aacactgaac actgaacatc gaatgatgaa gtgaagtgtg
     9541 aagtaaaaat caatgatgta tcgttcgata ttcttacttc cttgcctgcc tgactgcctg
     9601 ttgcagttaa accgtcagac aggttccttg ttcagagttc ttgacctcta ccttgttttc
     9661 attcctgttt caaaattatt ttttaaaaga ttgctgtatt tttcaaaatt tgcaatacat
     9721 tagcgctgat tttcaaacca attaaattac atctccgaaa atgaaaataa tcgtactcaa
     9781 ctgcggcagc tcctccatta agtatcagct gttcgacatg gacaaaaatg aacaggttct
     9841 ggccaaagga ttgcttgaac gtatcggaat tcctaacagt ttgcttaaac attcatctac
     9901 tggcaaagat tcttttgaag aaatcagaga tattccggat catactgtag gtattcaatg
     9961 gattatagat acactgctcg atcccgttaa aggttgtctg aaagataaaa ccgacatcaa
    10021 tgctgccggc catcgtgtgg cgcatggtgg tgaatttttc tctgaaagtg cttttattga
    10081 ttcaaaaaca aaagctgcga ttgagaaact gattgaactg gctccattgc ataatccggc
    10141 aaacctaaaa ggtattctgg ccattgaagc attgttgccg ggattgccgc aggtggccgt
    10201 tttcgatact tcgttccatc aatcaatgcc tgaacatgcg tttttgtacg gacttccgta
    10261 tgaaatgtac aaggataaaa aaatccgccg ctatggcttc catggcacca gccataaata
    10321 tgtagctgaa aaagcagcaa ctattttagg catggattgg acaaaaatga gaattattac
    10381 ctgtcatctt ggcaatggtt cttcaattgc agccattctc aacggcaagt caattgactc
    10441 ctccatgggt tacactccgg ttgaaggatt gctgatggga acccgcgttg gagatcttga
    10501 tctgggtgct atgctggcta ttatggaccg cgaaaaaatg tctaatgccg atgccaatgc
    10561 tctgatcaat aagaaaagcg gattgcttgg tatttcaggt atttcatccg atatgcgcga
    10621 tattgaaaaa gctgctgctg aaggaaattc tcaggccaat gttgctcttg atgtgtttgc
    10681 ttaccgtgtg aaaaaataca taggttcata catggctgcg ctaaacggac tcgatctgct
    10741 tattttcaca ggcggaatcg gtgaaaacga ctgtgacagc cgccgccgca tctgcagcaa
    10801 tctggaagga ctcggcattg aattcgatgt tgaaaagaat aaaggcctgc gtggaaaaga
    10861 tgccgttatt tcaaccgaaa attcaaaagt aaaagtaatg acagttacca ccaacgaaga
    10921 gctggtaatt gcccgcgata cgattcgcat cgttacaaaa taatttttca gaattctgat
    10981 aaaacctctg tagaataaaa gctgcagagg tttttttgtg cagaaaaaat gccttcagat
    11041 ttctccaaag gcattcacgg aatgtatttt caattatcct ttcaacgctt cagcgccact
    11101 tacaatctca atgatttcgt tggtgatggc cgcctgacgc gccttgttgt aactcaggcg
    11161 gaggtctttc agaatttcgg ttgcgttgtc ggtggcctta tgcatcgatg tcatacgggc
    11221 gccgtgctcc gaagcaatgc tgtcgagcag tgctttgtaa agctgaagtt tcaatgtttt
    11281 tggaacaata atggtgaaca ttttttcgcg accgggttca agaatataat cgttaccggc
    11341 tatttgtttt cctgattcca gtttgaatgg cagcagcatt tcgctgaccg gaacttgctg
    11401 tgcagcatta cgaaactgat tgtacacaag tacaatggcg tcgtattttg catggatgta
    11461 ttctttaacg atgttgtcac aaattccgga aacttcttca aacgaaggtt tgtcgatgag
    11521 atgattgtaa tcttttgaaa aacggcaccc tttcttttta aggagatccg gagcttttcg
    11581 tccgaatgaa atgaaatcaa ttttcccggc ttcaaaactt ttccggtatt cgccttcaat
    11641 cagatgattg atttgtttta cgatattggc attgaaaacg ccacagagac cgcggttggc
    11701 agaaaaaaca accatcagga ttttgtcggg attgcgttgt gcaaaaacgc catcagtcgg
    11761 caggttatca acgccggcag caacgcggcc catgatttcg cgcagcttcg aagcgtatgg
    11821 cctgaagcgc agaatattat cctgtgcttt gcgcagtttg gatgcagata ccagcttcat
    11881 ggccgatgtg atctgcttgg ttgttgtaac cgactgaatg cggtttctga cttctttcag
    11941 attagccatt tcagatggtt ttagtggtat cggcctgcaa tatcaagcgc aattttgtca
    12001 agttttgcaa tcatttcgtc ggaaagtgct ccttttcgga gttcatcaag caaatcggca
    12061 tgattgtctt cgaggtgatg cagaaattct gattcaaaat ttttcacctg tttcacagga
    12121 acacttttaa gtaatccgcg ggtaccggca aaaatgatgg caacttgctg ttcaaccggc
    12181 ataggcgagt actgaggttg tttcaggatt tccacgttac gggtgccttt ttcaagaacg
    12241 gctttggtag cggcgtcgag gtcggatccg aatttcgaga atgcttcgag ttcgcggaat
    12301 tgtgcctgat cgattttgag tgtcccggca atttttttca tggccttaat ctgcgcatta
    12361 ccacctacgc ggctgacgga aatacctacg ttgatggcag ggcggatacc cgagttgaag
    12421 aggtttgttt cgaggaaaat ctgaccatcg gtaattgaaa ttacgttggt tggaatgtaa
    12481 gctgaaacgt caccggcctg ggtttcgata attggaagcg ctgtcagaga gccacctcct
    12541 ttcacaagat gtcggatact ttcaggcaag tcgttcattt gtttagccac ttcgtcgctt
    12601 ttgatcactt tggcagcgcg ctcgagcaga cgtgagtgga ggtagaaaac gtcacccggg
    12661 taagcttcac gtcccggtgg gcggcgcagc agcagtgata cttcgcggta tgcaacggct
    12721 tgtttcgaca aatcgtcgta gatagccaga gctggtcgtc cggtatcgcg gaaatattca
    12781 ccgatggcgg cacctgcata aggagcatag aattgcagcg cagccggcat cgaagcggta
    12841 gctgaaataa taacagtgta gggcattgca cctttttctt caagtgtttt tgcaatctgc
    12901 gccacagttg aacctttttg tccaacagct acataaatac agaaaaccgg tttacctttt
    12961 tcgtaatttt cgcgctgatt gatgatggtg tcaatggcaa tggcggtttt accggtttgg
    13021 cgatcgccaa taataagctc gcgttgtcct cgtccgatgg gaatcattgc gtcgatggct
    13081 ttgatacctg tctgaagtgg ttctgtcact ggctgacgaa aaataacacc cggggctttg
    13141 cgttcgagcg gcatttcgta gagttcgccc tgaatggacc ctttgccgtc gatgggttca
    13201 ccaatggtat tgattacacg accaagcaat ccttcaccca cttggataga agcgatggtt
    13261 ccggtgcgtt taaccacatc cccttcgttg agatttttgg tattaccaag gagtacaata
    13321 cccacattat ctgcttcgag attaagcaca atacctttaa gacctgtttt ttcgaactcg
    13381 accatttcgc cggcgcgagc gttactgagt ccataaacac gagcaatgcc gtcgccaatt
    13441 tcgagaacgg ttcctgtttc ttcgagactg cttcccagat tcattccgga aatttgctgg
    13501 cgaagaatgg cggttacttc cgatggttta atttctgaca tatataccct tttttaaaat
    13561 cctttttcgt aaatgttggt tcggaaatct cttgagattt tcgaaatttt ctttttcaat
    13621 gaagcatcat acatgttgtc gccgaagctt accacaaaac caccaagtag tgaaggattt
    13681 gttttttgtg tgatgctggg tttgagcccg gtgagcgatg caatttttgc ttcaatttgt
    13741 ttgacaatag agtcgttgag ttttgtagca gcggtcagat cgacttcaac gacaccttct
    13801 ttttctctgc aaagaatcat gaattccttg gcaatttcat tcataaaggt ttcgcgaccc
    13861 tgatgaatga caagtttcag aaagcgcatg ctgagttcat gaaaatgttg ttcaaacaaa
    13921 gaagatatga cctttttttt aagagctggt ttaatgatgg gacttttcaa aaattgtttg
    13981 aattccggcg cttcatcaca aatttttttg agtgaacgca tatccagctc agtagccggc
    14041 aaaacattta tttcatcggc cagctcaaac agtgctctcg cataacgttt tgggatttgg
    14101 gagttgttca tggctgcgtt tagttaaatg aaatatcgcc agccattttt tgcgccagtt
    14161 cttgttgttt ggcgtccccg ctcagatttt cctgaatgat tttttcagca atttcgagtg
    14221 ataatacggc tacctgattt tttaattcag taatggctgc cattttttcg aagtgaatgt
    14281 tttcgtgagc tgcggcgata attcgttcag tttcttcttt ggctttaact ccggcctctt
    14341 caacaatttt gtctctgatt ttacgggctt cggccaggat aatgtctctt tctttttgag
    14401 cttctttgag caactgctcg ttgtttacct gcaattgcgt catttcctgc cgtgcttttt
    14461 ctgccgccat aagagcattg tcgatggctt cgtttctttt cttaattgct gcaactattg
    14521 gtttccaggc aaattttgaa aggagccaga ccagcagaag aaaaacaatg cttgtccaga
    14581 atattaatcc aagtccggga gttgtcaaca tattattttt tctttgggtt catgataaaa
    14641 agccccgatg ccaaccgcag ggggcttttg tttttacgaa aattacttgg cgatgagagc
    14701 aacaacaact gcaaaaaggg caacgccttc tacaagagct gcagcaataa tcatagccgt
    14761 ttgaatttta cctgttgctt cgggctgacg tgcaatggcg tccatggcag agccaccgat
    14821 acgaccaatt ccgattcctg cgccgataac ggctaatcct gctccgattc ctgctaaaac
    14881 catagtgatt ttattttaaa gttaaacatt ggttttttaa tgatgttctg cttcctgcac
    14941 ggccaggcca atgaaaagtg aagtcagcag tgtgaaaata tatgcctgca gtgcagcgac
    15001 cagcaattca agtacatcca tgaaaagtac aaaaacaact gaaactggcg ctattccgag
    15061 attctggaaa atgaaaatga gcgagatgag acttagcaca atgatatgtc cggcagtaat
    15121 atttgcaaag agacgaacca tcagagcaaa tggttttgaa atgataccga taagttcaac
    15181 gggaatcata attggtaaga gccagactgg cacaccaggt gctgcaaaaa cgtgtttcca
    15241 gtatcctttg ttaccactaa catttacaat aatcagagtg aagaaagcca gtgtcagtgt
    15301 tacggccaga tttcctgtca ggtttgctcc aaagggaaag aaaggaatca gtcccatgag
    15361 gttgttgatg agaataaaaa agaagagtgt tagcagatat ggaacatacc gttcatatct
    15421 tttttcgcct atatttggct ttacaatgtc atcgacaaca aaaagaatga gcggttccat
    15481 aaatccctgc agccctttgg gtgctgcatt cccggctttt ttataactgc gtgcaacagg
    15541 tataaaaata agtaatagta atgatgcact gaaaataaga gccgctacat ttttggttat
    15601 ggagaaatca agcggaacac tggcaacctg tccatcgtga ttgacccgga caattttgcc
    15661 ttcgtattcg ccattgaaat tatctttggt tccgagttga tattcgctgt gttcagcatg
    15721 atgaagacgg gaactcatga aaaaaaccag ccgcccttca tcccaaagaa tgaccggaag
    15781 tggaacaacg acatgttttt cgtttgtttc accttcattg agggtcatta cgtgccaatc
    15841 atgtgcatcg agaatatgat cgaagatcac ctgggtgatg tcgatgcctt cgttttgctc
    15901 tgttttgttt ttgtcatgac cgtggtcaga acccgcatac gctgataatc cgctgaatcc
    15961 ggcaaaaaca agtgtgaaaa tgaatattaa cttttgcatc acggttatta aatcgtgtgc
    16021 aaattaaaca atttttattt gttggaaaac atttgattgc tgaaattgcg cctttttttt
    16081 ctttttaaaa aaaataatca acaagtaata tggataaacg gcattgaaga ttgaatgaac
    16141 cggcaaaaat catcaatgaa tttattttgt ttgacgcatt tttataaaca gtggtaaaga
    16201 aataagaaaa agaaaaacag ctgaaagggc aaattcaacc atatatctta attgataatc
    16261 aacaacttcg gacatgttca catcggcaca cagcagatat ggcgttccgg tccttgtttt
    16321 tttcggcagg tatatgcttc tgaaatttcc ccattgatcc tgcgatgagt catagatttc
    16381 aggatcgttg ctcgaaaaag cattcatcat ggttggagtc gcttcgtaat aataatccag
    16441 ataatctgta acaatgtcgc cggtaatatc cgattcgatg tatgaagaaa aaacaaaaag
    16501 agccgaatcc tcagattcaa tcattacata tacatatata acatcgtgga ttttggcaat
    16561 tttgttggca agaactctta tgtattcatg atcgacaaaa tttaccggga ttttttgttg
    16621 tgcattgtca atcatggagt cgtttacaat catttcaagc gatccggcag cagatttcag
    16681 tcgtgtatca atttctgcag aagcaatgtt tttttggttg taaagcttca cccaaacaaa
    16741 aagaaccatt ccgacaacaa aaataattat cggaacgagg ggtttctttt ttctgtgatt
    16801 aagtctggcc atcgaaacac aaatgtaaat ggttttttga ggtgtacaaa taatttaaca
    16861 atttttacga agtatatttc ccgctaaagt tcaaaaataa gtgattagta cttcttaatt
    16921 gaattgtctt atggcttctg tttgaacttt ttactatgga tagcattcac ctgcacataa
    16981 atcaacggaa atgaaattaa aataatgtaa ataaatgaaa tcagaaattc ggtcagaacg
    17041 cggaataatt ctttgttgat gtctctgata aacttgtccg aacataacag atagttgatt
    17101 cctccttttg tttttcgggg tatataggca ctgcggaaag taccccattt gttgctgttt
    17161 ttgccgaaaa tgatattgtc gctcatccta aatgcttttt taagttcggg tggcgcttcg
    17221 gcaaatacat caagatattt gatttcaata ttgtttataa tatcagatgg cagaatgttg
    17281 caaatgacaa aataaaccga atctctgttt tcaataacca catatacata atcgacttcg
    17341 tgtatggagg caattcggtc aatttttttc tgcatgttgc tgtatcgatc tttgtctggc
    17401 tgtttgccgg ttgccgctgc ttcaataaga ctgtcgttga ttaccaactc tatggtgccg
    17461 gccgaggatt caagtctgaa attgatgttg ttggtgataa ttctttcgtg tatgaaaagt
    17521 ttaatggcca gcaacaataa aattcccaca ataaaaatta tgacaggtgt ccattttttt
    17581 attattctat tgcggtaatt cattatgcaa atatatcaaa taaatagact aatatcaaat
    17641 taattatatc taaatgcgat gtatttgatt ggaaaatatt cacggcagcc aaaaaatgag
    17701 tatcattccg gcatgtcgca tggatcaaaa aaccttattc agtttgtagc cagcttactt
    17761 tttgaactta tgataatccc aggcgtcgaa ctgttgttgt tttaaaactt cctgggttgt
    17821 gatggcaagt cgtagaaaaa cgtccatatt gttttttttt aacagctcct ttgctggtgt
    17881 aacaccgcgg catgcatcag caaatattat ataaaaggtc agattgtttt ttttgagcca
    17941 ggcaatggct gcatcttccg aatcaatggc gtcgctcaga atgccgagtt caggtgatcc
    18001 attgtcgaag agccatttca gtgctttttt gtcggcacgt acagcactgg caaatgcagc
    18061 cagttccgga aatccgtttt caagcagaaa ttgcagaaat tccgaattgc ctccaatggc
    18121 ctgttccaga gccagtagta tttttacttc gtattcccag agcatttttg ccataacgtt
    18181 attcttttcc ccattctatt tggggggttg atattccggt gatactgatt aaacgcgaaa
    18241 ccagcgccga aattgcttcg cgcaggtctt tcggatgatg gtagaaaaac ggcgaggccg
    18301 gaaaaacgat ggcgcctgcg cgggttaccc tttccatgtt tttaatgtga ataagactta
    18361 acggtgcttc gcgcgcgagg agtatcaatg gtctgcgttc tttcagaagt acatcggcag
    18421 cccgcaatat gagtgttcct gccgttcctg ctgccaccga tgccagtgtt cccatcgagc
    18481 atggtgcaat gaacatagcc tgatatccgg ctgagcctga tgcaggacag gcaaacatat
    18541 cgttgttttc aaacacggtg atttttttac tcaccggaat atccatcact ttttcaaaat
    18601 cccagacttt gcttccgttt tcgctgtaaa tgacggcaat gtcctttatg cattccaatg
    18661 caacaagctg tttgatgagc tcaagaccat aaacagcacc gctggcaccg gttatggcta
    18721 caatgacttt tgatatttta tcagaaccag tgttcatagg tcaaaccaag taacatattg
    18781 tattgtccgc ggataaaata attataggtt gatatcccgg gcgcttttcg gataggcaga
    18841 accgagttgg aataacgcag attgatggat tgcttttcat tgaaaaagta acggaaaccc
    18901 aaatgtgcgg ccagttcgta tttttcaaaa tcaggacgag cgggaattgc tccattgata
    18961 tcaaattccc tgttttcatt gtccaataat attccaaacg acaatcctgt ttcgaagcca
    19021 aagttgcttt ttaattgacg ccgaaataat acaggaactt caatgtaatt gaggcgcatg
    19081 acgtattttg tcaggttgtt gtctttggtt ggattgcggc ggctgccttt ttgtacgaaa
    19141 agcatttcca tggcaagatc agaaaattcg ttcagtggaa ttgctaccat gaaacctccg
    19201 gtcagtccgg ctttgtcaaa acccgaaagg ttgtctccat cgacttgtga ccctgtgagt
    19261 cctgcgcgga atccaccccg gaagctttgt gcgctgcttt gtaatgtcag tatcagcaga
    19321 aacagaaaca gaattttttt cataggtctc aaacgaatga cgaaggtagt aaaatacgaa
    19381 atacgaaatg agaaatacga aatacgaaat gagaaatacg aaatatgaaa taaaatgtgg
    19441 aaatgtggga cttcacgtga aacgtgacac ggtcgtccat gtgaaaatgt gggaagatta
    19501 gatgaccact tttatcttct tacttttatc ttttgtcttc agaggatgtg gaaatgtgtt
    19561 gcttcacgta aaatcacgta aaacgtgaca agctgtgaca cagtcgcaaa tgtgtaaatt
    19621 caatattctg caaaaaattg tcagggaata aatgcatttt caatatgatc gatccagaca
    19681 tccttgtcgg gggaaacaat gattgaaatg atatcgaaac gcatttcgcg gtcaatcgaa
    19741 aattcttcaa ggtaggcgag tgctgcacgt tcgatggctt gttgtttccg atcgtcaact
    19801 gattcccatg gctcgccaaa agcagtgcct gtgcgtgttt tgacttctat aatgacaaga
    19861 atctcttcat gaagtgccac aatatccagc tcatctttgc cgcaacgcca attcctgtgc
    19921 agaatttcgt agtttttgct ttgtaaaaac aaagcggctt catcttcccc gaagcgtcct
    19981 gtttctgatt tgctggtgct tttattcatg gcaccaaaag gttatttctt aatgaatgaa
    20041 ctgctgtatt gccagtcagc tgttgaaaca ctgatgcgat atgccccagt agccagcctt
    20101 tgaatatcaa cagattcatt tgcggatatt tcatctgaga taacaagcgc tccgcggctg
    20161 tcgtatatat gacagtttat gggttgattt tctgcaccgc tgatgaaaag ctgattttcg
    20221 gccggatttg gatacaggct gatttccgga acatcgtgct cttgtgtact taaaagtata
    20281 acctgattga atccggaaag gagctgaaat tcgggatcaa attgcattgt tgttggaaag
    20341 ctggtgtatg gaatccgaaa ttcttgtccg gaaaaggtgt tgtcaaagac ataaatactg
    20401 tcatcaatct ggatcggtac cggcatttcg aaaaagctga ccgaagagtg gctttgtgtc
    20461 tggtcaatcg caatagacaa ggaatcacct ggaattggca ttgcaacaat ggtgtaggag
    20521 ggatatcctt catgatagag ccagtcatcg aaaaatccag tcaggtccat gccggactga
    20581 gcttccatat gtgcaatgta atcggggctt ttggcgtatc cgaaatagaa gtttgcatca
    20641 ttgaaataat tgcggttgcc ctgaaagaaa ttgtcatcgc caactaccca acgcagcata
    20701 tgcagcatat aagctgcttt gttatagctg agccggctat cgaatacgcg gctgacatca
    20761 gatgtgtcat acacaaaaac agaaccgccg ggatcggatg ttacatgatt aaccgtattg
    20821 tttttccaga taatccaata ataatccggg cttgtccttt cataattcag cgctgtaaaa
    20881 tatgttgcaa agccctcgtt gagccagatg tcgtgccagg atccacaggt aacggcatcg
    20941 ccgaaccact ggtgaagcag ttcatgagcc atcagttcgt agccgaaacc gcccataaaa
    21001 ctcatggtct ggtgctccat gccaccgccc cagccaaatt gcgcgtggcc gtatttttca
    21061 gtcatgtatg gataaggagc aattagcgaa tcgtagaatt gcataacgtc ttcgagcaga
    21121 ggggtcatgt tttgagcatc cgtgagactt tccgggtaaa catagctcag catgggtatg
    21181 gtgccggtcg ataaagagaa atcatctgaa aaaatactgt aatcacttac tgcaatggca
    21241 atcagatagg cggctatggg ataacggtgt ttccagtgtg cggtggtggt tgctccaact
    21301 gtttccgaaa caagtattcc gtttgatgcg gcgtggtaac cgttgggatg ggttacatag
    21361 atatcgattg aatcgatttt gtcgctcaga tcgtttttgc agggccacca ttcgtaggcg
    21421 ccatagggtt cggagagtgt ccaaagtgcg ggagttccac ttccatgtgt gcttgcaacg
    21481 aatgaaataa agccgcttgt tgcctgaggt gtgccgtgat aataaacagt tatgctgtca
    21541 agtatgccag ctgataatgg cgggaaccct gtgatgttga cttcattgtt gacatgtacc
    21601 gacattgccg gtgctccgtg atatttcacc gaatcaacca tcatatttac tttaagcgaa
    21661 aagtcgattt gtgtcaggtt attctgaaca ggtataaaat atgaggtaac cgagccgctg
    21721 atgtagcaag tgtccggatc aacattccag ttgaagcggt gatatttcag gtcgtattcc
    21781 tgtccgacaa atttttccgg aacatcaagc attctggatg ctttgaaagc atcgtcgagt
    21841 gcaatttgct tggtggcgtt gatgttgaaa atctgtgcat gagtgtttag cgccagaaaa
    21901 gccagactta ttgcaaaaaa gagtttgtgc atggttgaaa ctttattgta agagcaaatg
    21961 taagataaat tcgttgcagt gaaaaaatta ctgccttccc aaaaactaaa atccctcgaa
    22021 ggggttcaaa cccttcgagg gatgtagtta acagtaacaa gtcatatttt tagtacatgt
    22081 cgcccattcc accaccaggc attggcggca tagcgggttt gtcttctttg atttcgacga
    22141 taaccgtttc agttgtcagc agcataccag caatggatgc ggcattttcg agagcaacac
    22201 gggttacttt ggtcgggtcg attacacctg tttcgagcag gttttcgtac acttctgtgc
    22261 gagcgttaaa gccataatca gcttttcctt ctttcacttt ctgaacgatt acgctgcctt
    22321 cgagacctgc attttcaaca atcatgcgca tcggttcttc gatggctctg cgaatgatat
    22381 cgataccggt tttttcgtct tcgttaccgc cggttagacc tgcaatggct tcgatggcgc
    22441 ggatataagc cacaccgcca ccaggaacaa taccttcttc aacagcggca cgggttgcgt
    22501 gcaaagcatc gtcgaagcgg tctttctttt ctttcatttc gacttcggaa gcggcaccta
    22561 ctttaatcac agcaacacca ccagccagtt tggccagacg ttcctgcagt ttttcgcggt
    22621 cgtaatcgct ggttgttgct tccatctgag ctttgatctg agcaacgcgg gcatcgatgt
    22681 tttctttgct ccccaaccct ttaacaatgg tagtattttc tttgtcaacg gttacttttt
    22741 cggcttgtcc gagatagctc aggtcagcat cttcgagttt ataacctttt tcttcgctga
    22801 taacaatacc gccggtaagt acagcgatat cctcaagcat ttccttgcgg cggtcgccga
    22861 atccgggcgc ttttacagct gctacgcgga gcgaaccacg gagtttgttc actacgagag
    22921 ttgccaatgc ttcaccttcg atgtcttcgg agataatcag cattggacgg ccggtttgaa
    22981 caactttttc aagaatgggc agcaattctt tcatgctgct gatttttttg tcgtaaatca
    23041 gcatgaaagc attatcgaac gaagtcagca ttttttcggt atctgtcaca aaatacggtg
    23101 aaagatatcc gcggtcaaac tgcatacctt caacaacatc aacggtggtt tcggtgcctt
    23161 tggcttcttc aatggtgata acaccttctt ttttcacttt tttcatggcc tcggcaatct
    23221 gtttgccgat ttcattatcg ttgtttgccg atatggtagc aacctgttca attttttcga
    23281 agctgtcgcc gatttccttt gattgttttt taaggttttc aattacggca gcaactgctt
    23341 tgtcaatacc tcgtttcaga tccatcggat tggctccggc agtaacgttt ttgagtcctg
    23401 tgttaaaaat ggcctgagca agaacggtag cggtagtggt tccgtcgccg gcagcatcgt
    23461 tggttttgct ggcaacttct ttgaccatct gagcgcccat attgtttacc ttttcaggaa
    23521 gttcaatttc ctttgcaacg gtaacaccgt ctttggtgat agcgggagct ccgtatgatt
    23581 tttcaataat tacattgcgg cctttaggac ccagggtaac ttttacggcg tttgataaag
    23641 cgtcgacacc ttttttcagt agttcgcggg cttcaacgcc atagagaatc tgttttgcca
    23701 tagtatttta agttttaaaa attttgaatt taaatgattg cgagaacgtc ggattccttc
    23761 atgatcagat aatccttccc atcaatggtg atttcggttc cggcatattt gccgtagaga
    23821 acaacgtcgc caaccttaac ggttactggc tcgtcttttt ttccggggcc tgcggcaaca
    23881 atggttccgc gctgaggttt ttccttggca gtgtcgggga taataattcc gctgctggtt
    23941 ttggtttcag cctgtgccgg ttctattaca acgcggtcgg ccaatggttt gatgtttatc
    24001 gtgctcatat ctgtcatttt tttgttcggc gcgccttgtc attttttatg ccaaaggtgg
    24061 ttatctgaca tttttgcagt aaatacatga caatttattg ggtttcgaac agattgttgt
    24121 ctttttgtca aaaaatttga aagtgtatga aaataaaaaa cccggagacc gggtttttca
    24181 attcttttgt tttgtctttt aatgacttat tcaacaggag ttgcaggttg ctcgccctga
    24241 ggtgtgttga ttggagccag ttcgttcgat tcaatgtaat tcttaacccg gctttcttcg
    24301 gtttgctgtt ggcctcgcgg tataacaaat atggaaagaa gactcagcgc aagaatgatt
    24361 atagccagag tccaggtgct tttttcaaga aaatcagcgg tctgacgaac acccataaac
    24421 tgattggatg actgcatgcc ggcaaccagt ccgcctcctt ttgaattctg cacaagaacg
    24481 atgagtccaa gaaggacaca aatgataatc atcaaaatca cgagaaaaat gtaaaagccc
    24541 atcgtattga tatttaaagt gttacaattt ctttttcaag ttttcaatca ggggtgcaaa
    24601 gtaactactt ttttccggat tagccaaaag taatttttca tacatccgca atgctttttc
    24661 gtagcgacct tgcttttcat aaatggatgc cagtgtttct gaaactacat caaatttttc
    24721 aacggtactt tcttcggcta gatttcgtcg gtcgggcaaa cggttcagat caatttttat
    24781 ggtaggttca gttttgataa actgttcgat gatttcagtt gtttctttct ccatttccgg
    24841 ctgtttttcc tggtccagtg gctcttcgtt tttagtgata ttcagttctg ccagtcgttg
    24901 ctgaagaatt ttcagcggat cacgcccttc ttcaactgtt gaattctgat ttttttcgtt
    24961 catctcttct tttacttctt caggattaag cattgttgcg gaaacttcgt cctgaatctg
    25021 aacttcaaga gtgttttcat cctgttcctt ttgcggctct gcaacttcaa cagcggtctt
    25081 ttccatttcc tgaacaacag cttccggttc ttcctgaacc ggctcgctga caacgtcagt
    25141 ttctgaaatc acaggattgt tttcagaaat gttcacgatt gtttcttccg caacctgagt
    25201 ctcaataagc gattcaacaa tttcttcggg ttccggaata acaacaggct caattatttc
    25261 tttttcaata acggcttccg gtatcggatc aggtgcgaca tactcttctg gctctggcgc
    25321 tgaatagctg ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtatc
    25441 ggatcaggtg cgacatactc ttctggctct ggcgctgaat agctgttgtc tgcaatggtt
    25501 tcatgcaaat ttacatacaa tatgtaacga tccggaacct tgaaacacga ttgtttgagt
    25561 ctttcagcaa acaattcagg gttttccttt tttaattggg ataaaacaat ggtttcgaga
    25621 tgagccgaat atggatgttg ctccagtatt cgtgctgcat cggcggttgt aatatgaaaa
    25681 tctgaaatgc tcatgtgctt ggtttaccag tttacaagtg ctttgttgaa tatgtcgtcc
    25741 accaattctt cggtgatggt ttcaatcagt ccgtcttcga ccgatgccag acttgaactg
    25801 cttgaaaact gctgaaaacg cgaaaaacgg gattcaaaat tctttgattc gtccaatcgg
    25861 ttgaaaaaac gaacattgat attaactgtt agctgtgtca gttgtgatgt ttcgtttccc
    25921 tgaatggcaa ccggctgcaa tgtgtatccg gtaatttcgc cttcaaacgc caggtctccg
    25981 ttctgattta ccatggtcag tcgtgtctgg cccatgattt tatcctgaag cttttcggtc
    26041 atggttttgg ccagcgtagg attaataagg gttgcattgt tttcgaaata ctgaactgaa
    26101 aatgtttttg cttccggatg tatggatgct cccgtaaatg taacacgcag gcacgatgat
    26161 aaaaataccg gcaacagtag aaatcctatg gtgaaacgcc gtatcattct atgccgtatt
    26221 ctttgatttt acgatataat gtgcgttctg aaatgcccag ttctgttgcg gcatttttgc
    26281 gacggccgtg gtgtttttcc aaagcttttg caattatttc cttttctttt ttctccagcg
    26341 aaagactttc ttctatttct tctgaatagt cgatcggaat ttcattcaca gcttcccttg
    26401 gatgttggaa atcctgcgga ggtgtggaat attgcggttt gtagaattcc ggctgatatg
    26461 ggatgctttc tccggctgga atggtgcgtc ccggcatggt ggggtcaatt ccggtggcag
    26521 agatgatttc ggcaatcagc tttttcattt ccgccatgtc ttttttcatg tcgaacagga
    26581 ctttatagag taactcgcgc tcattggcgt gttcatcgct gttttcgaag tggcgcttca
    26641 gtaccggcaa catggtcgga ttgctgtcgc tgatatactt tgcaagggta tttggggtaa
    26701 cttccctttc ctgttcaatg atggaaatct gctccgtcac attcttcagt tggcgaacgt
    26761 tgcccgacca gtagttttgc cggagcatgt tttgagcttc ttcggtaaga atgagcgcag
    26821 gcattctgta tttttcggca aaatcggctg cgaatttccg gaacagcaat acgatatcgt
    26881 ctttgcgttc gcgaagtggc ggaatatgaa tgggaacagt attcagccgg taaaacaaat
    26941 cttctctgaa tttgccattg gcaatggctt cgcctaattt gacgttggtg gctgcaacaa
    27001 cccggacatc ggtcttgatg acttttgagg agccaacccg cagaaattca ccgctttcga
    27061 gcacgcgcag caggcgcacc tgtgtgctga gcggcagttc ggcaacttcg tccaggaaaa
    27121 tggttccgcc gttgacgact tcaaaatatc ccttgcgggc atccgtggca ccggtgaatg
    27181 atcctttttc gtgaccaaat aattcgctgt cgatggtgcc ttcgggtatg gcgccgcagt
    27241 tcactgcaat gaaaggcccg tgtttgcggg cgctgaactg gtgaatgatt ttcgggaaaa
    27301 cttcctttcc tgttccactc tcacctgtga tgagaacggt aaggtcggta ggggcaacct
    27361 gacgcgcaac atctatggcc cggtcgatca gtggtgactg tccgatgatc ccaaaacgct
    27421 gctttatgga tgtgatttcg ttcatctttt tcaaaaggag tgctaaatta caaaaaatat
    27481 gacaatatcg acatgtttta tgccattttt tcagcttttg ggggttggcc acgaaggccc
    27541 gcaactggta gaaatttgcc actgaggcac taagacacaa aaagaatttt gaaca
//
LOCUS       DAOPKE010000034        25545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2041_length_25545_cov_11.215222, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000034 DAOPKE010000000
VERSION     DAOPKE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_25545_cov_11.215222"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKY63_06180"
     CDS             complement(<1..1076)
                     /locus_tag="PKY63_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF00236.1"
                     /translation="MMKILQVCGGPSWGGIEMQTVKLALVLQNAGHSTCVFCSADGEI
                     FKNAVEKKLKTETGLFNPHTSWFHSLQCAKKIIRNFNPDIIHVQRSHDLSILTAALRL
                     MKCKTPLVFSRRMESRLKKKSPIHRYIYSRVNRVWCVSSFVRENFLATSPMKPEKVFV
                     MNNGIDLRIFNPERFDKSGMRAAYSIPLDAIVIGMTGRISPMKGHTEFIRAAAMLLEK
                     TERNLFFVMGGGASVGEDDYATEIFCLAKELLPKNKYAFIGQQPDLAGLLSTMDVYVF
                     PSHRESFGNVLLEAMAMQLPIVAVNAGGVPDMITSGENALCVRPQHPEEIYSSVHKLL
                     NNNHLSTQLAQEARKRALDFSSEK"
     gene            1360..1854
                     /locus_tag="PKY63_06185"
     CDS             1360..1854
                     /locus_tag="PKY63_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00237.1"
                     /translation="MKQTHCNHAPAMDALIIFAKKNSMLPEAMKKFIKKHHVMTLSVG
                     DAEGVWSAHCFYAYDSSLNAFVFTSDENTSHVKMFRSGRVVALSIALETRIIGKIQGI
                     QMTGNVYKPIDLEYEKCKKLYLKRFPYAIVMKTTFWIFLPDYAKMTDNAFGFGKKLIW
                     TGND"
     gene            2073..4733
                     /locus_tag="PKY63_06190"
     CDS             2073..4733
                     /locus_tag="PKY63_06190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPF00238.1"
                     /translation="MENSLLGQTQVKTENDSKSGGYDQLVQKRTRPNIFENHETKGNE
                     SERKVYLYEDALKQSTEYFNGDALAASVWVSKYALKDSQGNIYEATPDDMHRRIAREI
                     ARIEKNYNNPMSEDEVFEVLKNFRYIVPQGSPMAGIGNNFQIASLSNCFVIGSNKADS
                     YGGIMKVDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTSTGIVPFMERYSNSTRE
                     VAQDGRRGALMLSISVNHPDSESFIDAKMEQGKITGANVSVRIDDDFMNAVSSGKQYT
                     QKYPIHSEKPVFSKSIEASDLWGKIVHNAWKSAEPGILFWDTIKRESVPDCYADLGFE
                     TVSTNPCGEIPLCPYDSCRLLAINLYGYVENPFTENASFNMPLFREHVWIAQRIMDDI
                     IDMELEKVDAILEKIDKDPEPGDIKNTERQLWMKIKEKALQGRRTGIGITAEGDMLAA
                     LNMRYGSDDAIDFSVNIHKTLAVEAYKSSVNLAAERGPFQVFDFEREKNNPFINRLFN
                     EEPQLKESMKQFGRRNIALLTIAPTGSVSILTQTTSGIEPVFLTAYKRRKKVNPNDKN
                     SNATFIDESGDSWEEFNVFHPKFLTWAGVNGYDVDEVKYHYSEEQLNDLIAKSPYHKA
                     TSNDVDWISKVKMQGAIQKWVDHSISVTVNVPEHVDESMIDMVLRTAWESGCKGVTVY
                     REGSRSGVLVADNKKKEETCSQFTETSAPARPVRLDASVLRFNNGAEKWIAVVGLLND
                     RPYEIFTGRADNFVLPEFVHRGWVIREKNRDERARYDFEFLDKDGYAVTIRGLSRTFS
                     PEYWNYAKLISGVLRHGMPIPYVVQLVDNLQFDNDDINSWRTGVVRALKLFIPDGTKA
                     QGECPQCHTDALQFKEGCVTCSNCGYSKCG"
     gene            complement(4851..6557)
                     /locus_tag="PKY63_06195"
     CDS             complement(4851..6557)
                     /locus_tag="PKY63_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPF00239.1"
                     /translation="MKNIYLMAFMAAISLIIISSCGNKKDKGGDMKSELQAFIKKYED
                     TIRPVMKEYTTAMWNSEISGSEADYAKVEEVGIRYSKIFTNKEDFALLKKIKDAGGVK
                     DSLLDRELLVLFNAYQRNQVDEKKLEEIISMETKIAKKMNTYRATTEKGDSITDNQVE
                     DILINSTDNKKLEETWKAHKKVGHVVAADIITLVKKRNAVAKDLGYNNYHEMSLKLSE
                     QDPAEIENLFNELDELTRDGFAKLKSQIDSFLSKRYNVPIAELKPWHFQGRFFQEAPK
                     IYTLDLDKYYKDKDLVKLTADYYASLGMDVKDIIDRSDLFEKKGKNQHAFCIDMDNEG
                     DVRVLCNVKPNYKWMNTMLHEFGHGVYDKYISRDLPLALREPAHTFTTEAIAMMFGRL
                     ASNAQWMQDMLGISDDEKAKIKDEAFKYMRLEQLVFSRWSQVMYRFEKGLYENPDQDL
                     NKLWWDLVEKYQLMKKPEGRNEPDWASKIHIATSPCYYHNYHLGELLASQLNHYIVVN
                     IMKQDDINGASYYNNKEVGKYLMDKVFAPGSQHSWNEMIKRATGEELTAKYYAEQFVD
                     GL"
     gene            7021..7536
                     /locus_tag="PKY63_06200"
     CDS             7021..7536
                     /locus_tag="PKY63_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00240.1"
                     /translation="MKHLVFFFIATLFALSSCSVFDMQSKARHTYLNKVPVNESSVDV
                     RTEKAVFCDPQPDVSALASLPENICETNQSPQQINTVQDKASDYSAKTIPAVVFVRDF
                     EKTFRTRARAHSADDSFSIDPATLLIWVLVVAIILALLGYLLPVLWTVLIYTLAVVLI
                     IMAILFVVSNL"
     gene            complement(7591..8511)
                     /gene="queG"
                     /locus_tag="PKY63_06205"
     CDS             complement(7591..8511)
                     /gene="queG"
                     /locus_tag="PKY63_06205"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPF00241.1"
                     /translation="MQKLESEITELLLQQGFLAVGFSEAGPVHSSWQKKFKDWIKAGN
                     NSAMQWLERNTDKRTDPTLLHPGTQTIISMLHLWPETEFDSQTIKIAAYAHGSDYHLY
                     LKKKAEPVIKLIAAADPAFRPVFITDSAAVFDRYWAWKAGLGFIGKNGFLINPMAGSR
                     VFISHIFTSLVFNYNPTTLENSCGSCTKCIDNCPTQAFNGDGTIDARKCIAYHNIESK
                     EITPLSISEKNPGWIFGCDMCQTECPYNQKKINSVFSEKMKGKWMEPSTKKEWMDMSK
                     TQFECNFSHTPLQRAGLDKLKQNINDLTPG"
     gene            8639..10324
                     /locus_tag="PKY63_06210"
     CDS             8639..10324
                     /locus_tag="PKY63_06210"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPF00242.1"
                     /translation="MKRKFFSVFLVLAAFFMNSPAMACTNYIVTKGASADGSVMITYA
                     ADSHVLFGELYHWAPAVYPEGAMMDIYEWDTGKFMGQIKQARRTYNVVGNMNEFQVAI
                     GETTYGGLPQLESQKGAIMDYGSLIYIALQRSKTAREAIMVIANLMSEYGYASSGESF
                     SIADKNEAWIMEIIGKGNFGKGAVWVARRIPDGYVSGHANQARIQTFPLANGKTSITD
                     EQFAKISLPAIECVYSHDVIDFARKNLEFKGKDAEFSFSDTYNPISFDGARFCENRVW
                     SFFREVNSDMEKYLGYIMGHDLKNRMPLFILPNKKISQKDLMTYMRNHMEGTPLDMTT
                     DFGAGPYECPYRWRPMVWEVDGKTYVHERVTATQQTGFSFVTQSRNMADEVGGIIWFS
                     VDDAASTVYTPVYTSSREIPSTYARGNGAMMEWSDDAAFWIFNQVSNFAYTRYNAIHP
                     EIKVKQDSLENLYIKLVQDIDKKALELLSSDKTAAIMYVTDFSVKNADGLVHCWKKFY
                     QYLFMKYMDGNVKTYIPGQQNPKVEYPGYGEKWLRHIVKDNGEELQYIGAPSH"
     gene            10599..13382
                     /locus_tag="PKY63_06215"
     CDS             10599..13382
                     /locus_tag="PKY63_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00243.1"
                     /translation="MKRITTVLGVCVLLVFFSSSALAQKNEGQQRYQPRNSKGKSEMG
                     ISGALEYYKLVRGTPEISDIVNAHKAANQLPKGKAMTWSEIGPDNIAGRTRALLIDRF
                     NTGVLYAGGVSGGLWQSLSGGTSWHLIAPLAENYAVSCITQDINGIIYFGTGEGFGNG
                     NGTAAGSTGFVGGGVYKSTDATGTSFSVISSTVPTSLTSDWIFVYEMAAAPNSTRIYA
                     CTDGGLFITDDGGTTWVNPVIYPSTTPYGQVSHDVEVAPDGSVYAVVGDKVFYSPNGN
                     SGTFVDRSITGTTASSISRVEIAVAPSDPNYIYASVAKVDGSLQNIYRSIDAGQNWTI
                     IGPGGSDLFQVFGSNNQGYWDNIIKVFPNDKNHILVGGVDLWEWTDGGTWLQKSLWYL
                     NPTSAYYLHADHHDIVFHPTNSSILYHGSDGGVSKSIDGAQTFSTLNRRFGTVQFYHL
                     SCNPFDMVMGGTQDNGTLILPGNLTSTGTAYEFLGGDGGWSAMSYIDPNVVVGTVYYG
                     DMYRSDELGNSPKAFYSEAMNAPEELSSAWAGFVTPMYLDEQINDLNSPDSMYFFNTD
                     TVSIPAGTSVVAYSKKNNNFPFYYTIENTLAAGDSVLIQDRVQARFYIGLNGAVWMTK
                     GLHDFSGAPAWYKVANISGTAQCFAGSKDGNYLYVGTNMGNLYRISNLSAVKDSLTGY
                     VTSTHSVVECTRIASTSRAITSISVDPSNPENILYTLGMYGKTDYVYYSTNALDSIPT
                     FVNKTGNLPKNPVYASIIEMSNSDLVIVGTELGIFSTSDIGAASVSWVEDNETIGRVP
                     VYSLVQQIKNYYGTNNYGVIYAGTHGRGAFKSTSYLGIEDEQEATSMATIGIYPNPAE
                     TTAILSFNSDEKGSGIVNVYTIDGKLAASKQVNLKAGNNLVELNVSGLESGNYLIEVR
                     TNMASVSGKLIKK"
     gene            13445..14917
                     /locus_tag="PKY63_06220"
     CDS             13445..14917
                     /locus_tag="PKY63_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00244.1"
                     /translation="MKKFLSALFILMAVSVSAQEKIYAPTLTAPADAAVNQKIDALLD
                     WNPVAAAVKYEVNLDTSASFTNPQDVVVTYSAWQSSLLLFGTTYYWRVRAIDANDVIS
                     SWSVVRSFTTSAAPSPLWPAPNSHDASLCPQLKWTLMSNVTKYECQFDSSLTFASPWF
                     KSALVDSTNYFHVFGNDYGEKYYYRVRAIHIYDTSDWCSPVAFFTRDSVAFHPTSPLD
                     ADTAIHPVDSIRVRGIYGTNSYEVQFSVDSVFTAPQIFHWDSTAMWIKFSSGAYDTIA
                     RGPMDTIPFGTFYYRVRLMTDDDTTKWTAVRSLTTVKKVKMLAPADGATAVDPATSFS
                     WEAIRGAAYYILEYDTVPAFTNVTKVDVVGTSYTPAAQLFSQTNYYWRVRCVTNADTT
                     DLYDDFHFKTFWGVGVEENEVATWSVYPNPTSGVFYLNLESASSARIEILNLLGDVVY
                     SQNSLANGSNTIHIDNLNDGIYMMRLYVDGKLYTSRVVKK"
     gene            complement(15589..16668)
                     /locus_tag="PKY63_06225"
     CDS             complement(15589..16668)
                     /locus_tag="PKY63_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00245.1"
                     /translation="MKKVLSILILTATAYSFFSCSTELDINGNKKEVMVVYGILDAAS
                     STQYIKINRAFLGEGNALEYAQIPDSTLYPYLLDVKLDVVNSNGQSVLSTPLTADTVN
                     VYKDGGGVFFSGYQTYYRFDFKSNIAALATINTFTNDTTWLKSDYRYKLIITDNVTGN
                     IYESEAPGLSNILIETPPSSSTTISFYTDNQSNVKFSSVKNGKIYEGKFTFHYYEIYD
                     SNPTDTIEKTIDWNLATVKSDNTSGGELLYMNYSNWTFYDLLGQAIEQRDDVQRYTGY
                     VFLSITIGAEDLNTYMDVNQPSNTLIQDKPSFSNISNGIGLFTSRYSYRRSRPMKLNT
                     NTKDLLKTHPSTENLNFIGELPNLP"
     gene            complement(16769..17536)
                     /locus_tag="PKY63_06230"
     CDS             complement(16769..17536)
                     /locus_tag="PKY63_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPF00246.1"
                     /translation="MKVSVFIPCLTDQFKPETAQNLYKVLRHLGVEIHYPEQQTCCGL
                     LAYNAGHWDEARLAGEKFIHEFVSERYVIMPSAACAHMVKNNYNRFFYNTSLHLEYKK
                     LQTKVFELSDFLMNILHVSAWKGKFKAKVMLHVESSAKIDYKLREEPLQLMKMIEGLQ
                     LVGANQNKSDCGFGGVFSVMYSQLSAQIALDELKTALAAGAEYIITTETSCVLQLESV
                     AAKHGLEIKIMTITDLLAESLRFSDAVFNEESQIAIG"
     gene            17640..18323
                     /locus_tag="PKY63_06235"
     CDS             17640..18323
                     /locus_tag="PKY63_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00247.1"
                     /translation="MKKKLLFWSALALIFTFTSCDLLQEEPPTKTQMEGVWLVTKAYD
                     LNSAQDITQKVNFPLTGFHLSSDGTIISTAGPLMMYIVYGDSKFVNIASEIDQVFNYA
                     SLDFNGGEFFIGGGVQTRFTLEMKLEGLPGQKALTTLLELIGIGNDYLDVVVYHKFMD
                     VKVDFSEDYETMYWEIDNMTTAVYNTKDNYGNYVLWQGWPVNNFSKVQITLQKQVKDL
                     RDLVEEASN"
     gene            complement(18405..19079)
                     /locus_tag="PKY63_06240"
     CDS             complement(18405..19079)
                     /locus_tag="PKY63_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00248.1"
                     /translation="MKRKIQLLSVVIALIFCGCEKKETYHFSIHGKVINSYTGLPVSG
                     AFVGASLQSWVSSCGGYLDSTHSDYYGNFTIEFDYTQRGDFIDKIILSSNSPNTFDST
                     LSEFKYLSDDRPVISASEADNEQIIKVLPTGQIRMFISDSTWQIIDADSIEIQSPCQT
                     KRIIKNQSDLCVYFHVDASAISIFKWFYYKDGVQSAEISRSIFVPNAISPYWSYYGPF
                     SYEVTF"
     gene            complement(19097..21130)
                     /locus_tag="PKY63_06245"
     CDS             complement(19097..21130)
                     /locus_tag="PKY63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00249.1"
                     /translation="MKVPLKQIFFPVLILWGTISFAALNAPSLRCLDVKSNGDVELSW
                     VIPPDPTAVFDSYQIFHSTTLTGPYIMVGNVSLYTQNTWLHAGANAQINAGFYYMITV
                     SDAPALQYSTPSDTLQTMMLTALNPLNGTAPLSWNALHMPPLITSANYNIFYEFPAGI
                     WTAAGTSMTTSFIDTIDICNSQINFRIQQTDASGCMSVSSIDGDIYQNTIPPEIPVFD
                     SVSVNPTTQNAILGWQPSASEDTEGYIIYIFTSVWTPIDTVWGINTTSYENAASTADA
                     NSESYCIAAIDSCATTSPLTTGHSTIFLSSTVDPCSRSVNLDWTAYSGFPGIDEYVLY
                     FSENGGTWSVLDVVSNTTTTYDVLNLEDDSSYCFYVTAVSTNGFFSSSNNSCQFVDFP
                     ELPSYVYIRHASVETDGSVYLKWVNDRNVYVIGYSLQRSLTPDGPWTSIDYQSYTATP
                     EYTFTDATASADLHSVYYRVAVIDSCSNEAMYSDTARTIFLSGEPLSDLTNELFWNDY
                     EGWPTGVQEYRLYRMIDNVPDAAPIATFTPGAEYHIDDVASFYLSYGLFTYRLEGIES
                     AGNQLGYSDTTLSNQIEIQQLPRLFIANAFSPTGYNKTFCPFGVYIDHTTYSFIIFNE
                     LGEEMFNTTDFYECWDGTNDGAPALVGVYHYILQMTLPGDQHFTKRGHITLIR"
     gene            complement(21134..22426)
                     /locus_tag="PKY63_06250"
     CDS             complement(21134..22426)
                     /locus_tag="PKY63_06250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF00250.1"
                     /translation="MNNQNINRKQAPSAGLLQFSGFPDFSETKINNLIPCYSFENFNS
                     EVVRLDIVIGAGTWSQQKPLQASSMARMMMEGTKKYNAFQIAETIDFNGAYIDITASN
                     HFTTISIFAVKHILVRLFPLLISIMTESVFPEEEFRVILANNKQEFIIGNKKVLSIAR
                     RTLPSMLYGETHPYGKILKESDFDNISSDDLKQLYSQLPLGNARCYIGGNMDTDVIAS
                     LSEMMEQTKKHLITTDEKNFDIQPSSEKQRIIEIKDAVQSAIMLGTPVVGRSHPDFPL
                     LMLTNTILGGYFGSRLMKSIREEKGYTYGISSGIAVRPKASHLSIQTEVKSETTQLAL
                     DEIFREISRLSSEKVQMEELNIARNYIFGSLLRSMDGPFALTERLMTMHAEQLNPKDY
                     FRNYWDTIAKANPDDIQRMAKKYLNPDEMRVLIVGNKN"
     gene            complement(22460..23698)
                     /locus_tag="PKY63_06255"
     CDS             complement(22460..23698)
                     /locus_tag="PKY63_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF00251.1"
                     /translation="MLQFEKFTLSNGLKVLVYNDKTTPMAVINILYGVGARDEDENKT
                     GFAHLFEHLMFGGSINIPEFDTPLEKVGGENNAFTNNDITNYHITLPAQNIETAFWLE
                     SDRMLSLAFSEKSLEVQRKVVIEEFKQRYLNQPYGDAWLYLRPLAFTTHPYKWATIGK
                     DISHIENATMSDVREFYNKWYNPANAIMVVAGPLELKVVKSLAEKWFGNIDKPHTATH
                     QIPQEPVQNKKRELTLTRDVPFKALYQAYHMPARNHPDYKVCDMISDLLSNGASSRLY
                     QNLVKDKNYFSSLNAYISGDVDPGLFIVQGELSNGYSFDDVIPAIDKEIEEIKKGNIH
                     DREIEKVKNKIESTFEFSKTSCLNNAMNLALYEWMGDANQINHEVEHYKAITKDDICR
                     VANELFRKENLSQLNYEPAK"
     gene            23750..25528
                     /locus_tag="PKY63_06260"
     CDS             23750..25528
                     /locus_tag="PKY63_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPF00252.1"
                     /translation="MLRDDNVVRKPALYLKRLIRHKQFVLLLSIMVGLAAGLAAVILK
                     TSVHYIEAYVRDQANVDAENFYYLVLPVLGILITVFFTVYIVKDDISHGVSKILRALS
                     KGESRMKPHFMYSGLVGCSVTAGMGGSVGMEAPILATGAAMGSNIGEWFGQSHRTRML
                     LIGAGTAGALAAIFKAPIAGVIFAIEVLALEVSAASIIPLLISAVTGALLSTFMLGKN
                     IEFYFSIQEPFEYRNIFYYVILGIVCGLVSVYFLRTNSWIEGAVQKIRKKIWRIVIGG
                     GAVGLLIFLFPPLYGEGYAFMRMILSGNVHDLSNNSFFYNFDENSWIFVGYLGLILVL
                     KVAAMSLTTGSGGIGGAFAPSLFMGGITGAAFVKVVNLLGFTTIIQSNFILVGMAGLI
                     AGVISAPLTAIFLIAEITGGYALFIPLIVTSSIAYMTTRLWEPYSIYTSRLAVSGELV
                     THDRDKSALTLLRVNEVVETDFKVVHPDQTLRDLVQVISASNRNLYPVVDEYCNYLGV
                     ITLDSVRKLMFDQSKYDTVLLRNLMQKAKESVSLNDNLDTAMGKFRKTGWYNLPVLNG
                     EKYVGFVSRSNILMIYRNKIREFSAE"
BASE COUNT         6910 a         4990 c         5682 g         7963 t
ORIGIN      
        1 aacttctctg aagaaaagtc gagtgcgcgt tttcgggctt cctgagccag ttgtgtggat
       61 aaatgattat tgttgagtaa tttatgtacc gaagaataaa tttcttccgg atgctgaggc
      121 cgcacacaga gcgcattttc gccactggtg atcatatcgg ggactccacc ggcattcacg
      181 gcaacaatgg gcaactgcat ggccatggct tccaaaagca cattgccaaa cgattcgcgg
      241 tgcgacggaa atacatatac atccattgtt gaaagcaatc ctgccagatc gggctgttgg
      301 cctatgaatg catatttgtt ctttggcaac agctctttgg ccagacagaa tatttctgtt
      361 gcataatcat cttcacccac gctggcaccg cctcccatca caaaaaataa atttcgctcg
      421 gttttttcaa gcagcatggc tgcagcccga atgaattctg tatgaccttt cataggtgaa
      481 atgcggcctg tcatgccgat tacaatagca tccaacggaa tgctataagc tgcgcgcatg
      541 ccggatttat caaatcgctc cggattgaaa atgcgcagat cgattccatt gttcattaca
      601 aaaacttttt caggtttcat gggcgaagtg gccagaaagt tctcacggac aaaggaagaa
      661 acgcaccaga cacgatttac acgactatat atataacggt gtataggtga cttttttttc
      721 agccggcttt ccatacgccg cgaaaagacc agcggggttt tgcatttcat caggcgcaga
      781 gcggctgtta gaatagacaa gtcgtgcgaa cgttgaacat gaatgatatc cggattgaaa
      841 ttccggatga ttttttttgc acattgcaat gaatgaaacc atgatgtatg tggattgaac
      901 aaaccggttt cagtcttcag ttttttttca acagcatttt tgaaaatttc tccgtcggct
      961 gagcagaaaa cacaggtgct gtgtccggca ttctgcagaa ccagcgccag cttgactgtc
     1021 tgcatttcaa tgccgcccca cgaaggcccg ccacaaacct gaagaatttt catcatgcaa
     1081 atatataaat attaaggtgt cggaattttt tcgttaggcg ttacagacgt tacacgtgga
     1141 atttattccg cttgaagcgg aaatcgttac aaggtcacgc gtcagagtca cgcgtcagga
     1201 gacgcgcgcc cagttcaagg tcgagattaa gatgttatat catagctgtc agcattcaga
     1261 aaggttgaga ttctggagcc gaaggcacaa aagaagctgc gaagcacaaa ttgcgctgaa
     1321 ggcgctgcat atgttggccc gggacaaagt cccggaagat tgaaacaaac gcattgcaat
     1381 cacgcaccag ccatggatgc tttaattatc tttgcaaaaa aaaacagcat gttgccggag
     1441 gccatgaaaa aatttattaa gaagcaccac gtcatgacgc tctcagtcgg agatgcagag
     1501 ggtgtatgga gtgcacattg tttctacgcc tatgattcca gtcttaatgc ttttgttttc
     1561 acttcggatg aaaatacgag tcatgtaaaa atgttccgat cggggcgggt ggtggccttg
     1621 tcaattgcgc tggaaacccg cattattggt aaaattcagg gcattcagat gactggaaat
     1681 gtctataaac cgatagattt ggagtatgaa aagtgtaaaa agttatattt gaagcggttt
     1741 ccatatgcca ttgtgatgaa aacgacattt tggatttttt taccggatta tgccaaaatg
     1801 accgataacg catttggctt tggcaagaag ctcatatgga ccggaaacga ctgattgtga
     1861 gccaggcaaa atacatctga attatataga tacttaataa aattcgtcta ttcagcatat
     1921 taaccttgtc agttgaaaag taaagcttat tgttgataat ttgggctttg cctaacaaac
     1981 agttgttttt attttagcca tccaaaacta cagtcattga gaattttctt aatggttgaa
     2041 attcatcggg ttaacttaat caacattgag caatggaaaa cagtttgtta ggacagacac
     2101 aggtaaaaac ggaaaatgac tctaaatcag ggggttatga tcagttggtt caaaaacgga
     2161 ctcgtccgaa tattttcgag aaccacgaaa ctaaagggaa tgagtctgaa agaaaagttt
     2221 atttatatga agatgccctt aaacagtcaa ctgaatattt caatggtgat gcactggcgg
     2281 ccagcgtctg ggtaagtaag tatgcactga aggacagtca gggtaatata tacgaagcta
     2341 ctcccgatga tatgcatcgt cgtattgccc gtgaaattgc ccgtattgaa aagaattata
     2401 ataatccgat gtcggaagac gaagtattcg aagtgttgaa aaacttccgt tatatagttc
     2461 cgcagggaag tccgatggct ggtatcggca ataattttca gattgcaagt ttgtccaact
     2521 gttttgtaat tggatcaaac aaagccgatt cgtatggtgg tatcatgaaa gttgatgaag
     2581 aacaggtaca gcttatgaaa cgccgtggcg gtgtcggtca cgatctcagt catatccgtc
     2641 ccaagggctc acctgtgaaa aacagtgccc tgacttcaac cggtatagtt ccttttatgg
     2701 aacgttacag caattcaacc cgtgaagtgg ctcaggatgg ccgccgtggt gcgctcatgc
     2761 tcagtatttc agtcaatcac ccggattcgg aaagttttat tgatgcaaaa atggagcagg
     2821 gtaaaatcac cggagccaat gtttctgtcc gtattgacga tgatttcatg aatgctgtct
     2881 cttcgggcaa gcaatacaca caaaaatatc ccattcattc cgaaaagccg gttttttcca
     2941 aatccattga agcaagtgac ctttggggaa agattgttca taacgcatgg aaatcggctg
     3001 aacccggaat ccttttctgg gataccatta agcgcgaatc ggtgccggat tgttatgccg
     3061 atcttggctt tgaaactgtt tcgacaaatc catgtggcga aattccgctt tgcccctatg
     3121 atagctgtcg tttactcgcc attaatctgt atggttatgt tgaaaatcca ttcacagaaa
     3181 atgccagttt caatatgccg ctttttcgcg aacatgtctg gatcgctcag cgtatcatgg
     3241 acgatatcat tgatatggag ctggaaaaag tcgatgctat tcttgagaaa atcgacaaag
     3301 atccggagcc gggagacatt aaaaatacag aacgtcagct gtggatgaaa attaaagaaa
     3361 aagcattgca gggacgtcgt accggaattg gcattacagc cgaaggtgat atgctggccg
     3421 cactcaatat gcgctatgga tctgacgacg ccattgattt cagcgtgaat attcataaaa
     3481 cattagctgt cgaagcttat aaatcatcgg ttaaccttgc tgcagagcgg ggtccgtttc
     3541 aggtgtttga ttttgaacgt gaaaaaaaca atccgtttat taatcgtctc ttcaatgaag
     3601 aaccgcaact gaaagagtcg atgaaacagt tcggacgtcg caatattgct ctgctaacca
     3661 ttgctcctac tggtagcgta agtattctta cccagactac ttcagggata gagccggttt
     3721 ttcttactgc ctacaagcgc agaaagaaag ttaatcccaa cgacaaaaac tccaatgcta
     3781 cttttattga tgagtcaggt gacagttggg aagaattcaa cgtgtttcat ccgaaatttt
     3841 taacctgggc tggtgtaaat ggttatgatg ttgacgaagt aaaatatcat tattccgaag
     3901 aacagctaaa tgaccttatt gccaaatctc cttatcataa agctaccagc aacgatgttg
     3961 actggatcag caaggtgaaa atgcagggtg ctattcagaa atgggtcgat catagtatca
     4021 gtgtaacagt caatgttcct gaacatgttg atgaatcgat gattgatatg gtgcttcgaa
     4081 ctgcctggga aagtggttgc aagggtgtca ccgtttatcg tgaaggatct cgttcgggtg
     4141 ttcttgttgc tgataataaa aagaaagagg agacttgctc gcagtttacc gaaaccagtg
     4201 ctccggcacg ccctgtaaga cttgatgcaa gtgttttgcg ttttaataat ggtgctgaaa
     4261 aatggattgc tgtcgttgga ttgctcaacg atcgccccta tgaaattttt accggtcgcg
     4321 ccgataattt tgttttgccc gaatttgttc acagaggttg ggtcatccgc gagaaaaacc
     4381 gtgatgaacg tgcacgctac gatttcgaat ttcttgataa agacggatat gctgtaacta
     4441 ttcgtggtct gtccagaaca ttcagccctg agtattggaa ttacgcaaaa ctgatttcag
     4501 gcgttcttcg tcatggtatg ccgattcctt atgttgttca gttggttgat aaccttcagt
     4561 ttgacaatga tgacatcaat tcctggcgta cgggtgttgt acgtgcgctc aaactcttta
     4621 ttcctgatgg aacaaaggcg cagggcgagt gtccgcagtg tcataccgat gcactgcagt
     4681 ttaaggaagg atgtgttacc tgcagcaatt gtggttattc aaaatgtggt tgattctgct
     4741 tacatcaata tagtataaac gggaccttcg ggtcccgttt ttcgttgtgt gcgaaaaaag
     4801 ctgcccggaa accgaacagc tttttttcaa atatttgaaa ttgggttttt ctacagacca
     4861 tcaacgaatt gttctgcata atattttgca gtcagttctt cgcctgtggc gcgcttgatc
     4921 atttcgttcc aactgtgttg cgatccgggc gcaaacactt tgtccatcag atatttgcca
     4981 acttctttat tgttgtaata gcttgcacca ttgatgtcgt cctgtttcat aatattgaca
     5041 acaatatagt gattgagttg actggcaaga agttctccaa ggtgatagtt gtgatagtaa
     5101 caaggtgagg ttgcaatgtg aattttcgaa gcccagtctg gttcattgcg gccttcaggt
     5161 tttttcataa gctgatattt ttcaaccaga tcccaccaca atttattcag atcctgatca
     5221 ggattttcgt acagtccttt ttcgaatcgg tacataacct gcgaccagcg gctgaatacc
     5281 agttgttcga gccgcatgta tttgaaagct tcatctttta tttttgcttt ttcatcatcg
     5341 ctgatgccca gcatatcctg catccactga gcattgctgg ccagacgccc aaacatcatg
     5401 gcgatggctt cggtagtgaa ggtgtgtgct ggttcgcgca gtgcaagcgg caggtcgcgg
     5461 ctgatgtatt tgtcataaac tccatgtccg aattcatgca acatggtatt catccatttg
     5521 tagtttggtt tcacattgca aagaacgcga acatcgcctt cgttgtccat atcaatgcag
     5581 aatgcatgtt gattcttacc cttcttttca aaaagatcgg agcggtcaat aatatctttc
     5641 acatccattc ccagactggc ataataatca gctgtgagct ttacaagatc tttgtctttg
     5701 tagtatttgt caaggtccag agtgtaaatt ttcggtgctt cctgaaaaaa acgtccctga
     5761 aaatgccatg gttttaattc agcaatggga acattgtagc gttttgacag gaatgaatca
     5821 atttgacttt taagctttgc aaagccgtca cgggtgagtt cgtccagttc gttgaaaagg
     5881 ttttctattt cggcaggatc ctgttcgctg agcttgagac tcatttcgtg gtagttgttg
     5941 tagccaaggt ctttggcaac tgcattgcgt ttttttacca atgttataat gtcggcagca
     6001 acaacatgac ctactttttt gtgggccttc catgtttctt ccagtttttt attatcggtg
     6061 ctgttaatca gtatgtcttc aacctgatta tcggtaatgg agtcgccttt ttcggttgtc
     6121 gccctgtagg tgttcatttt tttggcgatt tttgtttcca tgctgataat ctcttccagc
     6181 tttttctcgt ccacctgatt gcgctggtat gcattgaaaa gcacaagcaa ttcgcggtcg
     6241 agcagactat ccttgactcc gccggcatct ttgatttttt tcaacagtgc aaagtcttcc
     6301 ttgttggtga aaattttgga atatcggatt cccacttctt ctacttttgc ataatctgct
     6361 tccgagccac tgatttccga attccacatg gcagtggtgt attctttcat aaccggcctg
     6421 atggtgtctt catatttttt gatgaaggcc tgaagttcac ttttcatatc tcctcctttg
     6481 tcttttttgt ttccgcagct gcttataatg ataagactta ttgctgccat aaacgccatt
     6541 aaataaatgt ttttcatatt cctggttttt gtctggtttg agcgcgcaaa ttacagatat
     6601 ttttatttga gattgttttt ttatttcatt tttttatcaa tgggttgctt ggttttttgt
     6661 tttaatgatg tttcgtttgt aatttttatg atcatcctta ataatgccag tgattgactc
     6721 ataatccaat agaaagagtg tgaaaacaga atatatgtgt ggcgatgatg cgacaaatca
     6781 aatttcgcat aatcgcatta acaccacagt atgaataaat atctgactct ctcatctcct
     6841 ccattgcatt tcggactatt gaaggtcgtg ttgtttttga aaagaaataa atttcagaca
     6901 gtctctgaag ctttttctgg tattattgcg gatgtgcaaa taatttttta tccgtagttg
     6961 cgtttatttg taaaccgatt cgttgatttg tcttattttt gcaaaactca atttttctgt
     7021 gtgaaacatt tggttttttt ctttattgct actctgtttg ctctgagctc ttgttctgtt
     7081 tttgatatgc aaagcaaagc cagacatact tatcttaaca aagtgccggt taatgagtcc
     7141 tctgttgatg ttcgtactga aaaagcagtt ttttgtgatc ctcagcccga cgtttcagct
     7201 ttggcttcgt tgcctgaaaa tatttgtgaa accaatcaaa gtccgcagca aatcaatact
     7261 gttcaggata aagcatcaga ttattctgcc aaaacaatac ccgccgttgt atttgtccgt
     7321 gatttcgaaa agactttccg tactcgcgca agagcccatt cggccgatga ttcattcagt
     7381 atcgatccgg caactttact gatctgggtg cttgtggtgg ccattattct ggctttgctc
     7441 ggatacttgc tccctgtttt atggactgtt ctgatttata cgctggcagt tgttttgatt
     7501 ataatggcta ttttgtttgt tgtaagcaat ttgtaacttg ttcgccaaat aatacttctt
     7561 tttaatgtat gtctccctgc gactggctta ttaaccgggt gtcagatcat tgatgttttg
     7621 ttttagtttg tcgagtccgg cacgctgaag aggggtgtgc gaaaagttac attcaaattg
     7681 ggttttgctc atgtccatcc attctttttt cgttgatggt tccatccatt ttcctttcat
     7741 tttctcagaa aagacggaat ttattttttt ttgattgtat gggcattcag tctgacacat
     7801 atcgcatccg aaaatccatc ctgggttttt ttcactgatg gaaagaggtg tgatttcttt
     7861 ggattcaatg ttgtgatatg caatacattt ccgggcatcg atggttccgt cgccattgaa
     7921 tgcctgtgtg gggcagttat caatgcattt ggtgcaggat ccgcaactgt tttcaagcgt
     7981 ggttggatta taattgaaaa caagtgatgt gaaaatatgt gaaatgaaca cccggctgcc
     8041 tgccattgga ttgataagaa agccattttt tccgatgaag cccaatccgg ctttccatgc
     8101 ccaataacga tcaaatacag cggcgctgtc ggtaataaaa acgggtctga acgctggatc
     8161 tgcagcagca ataagcttta taaccggctc cgcctttttt ttcagataaa gatgataatc
     8221 tgaaccatgt gcataagcgg caattttgat tgtttgggag tcaaactccg tttcaggcca
     8281 gagatgcaac attgaaataa tggtttgtgt tcccggatgg agcagggtag ggtcggttct
     8341 tttgtctgtg tttcgttcga gccattgcat ggcggagtta ttgcctgctt tgatccagtc
     8401 tttgaatttt ttctgccatg atgaatgaac tggaccagct tctgaaaatc cgacagctaa
     8461 aaaaccttgc tgcaataata attctgtaat ttctgattcc agtttttgca cctgtttttt
     8521 gtttcaaaag tacacaattc tgaaaaaaag catttttgtt ttaaaaacca gataaaaatc
     8581 aggagtgttt gtgtaattca gaaaattcat gtaggtttgc cgaaacaatt ttacaagtat
     8641 gaaaagaaag tttttttctg tatttctggt actagcggcg ttctttatga attcgcctgc
     8701 aatggcttgc acaaactata ttgtaaccaa aggcgcttcg gcagatggat ctgtaatgat
     8761 aacctatgca gctgacagcc atgttttgtt tggcgaattg tatcattggg ctccggctgt
     8821 ttatcccgaa ggcgccatga tggacattta tgaatgggac accgggaaat ttatggggca
     8881 gatcaaacag gcccgtcgta cttataatgt tgtcggaaat atgaacgaat ttcaggttgc
     8941 cattggtgaa actacttatg gcggactgcc tcagctcgaa tcgcaaaaag gtgccattat
     9001 ggattacggc agcttgattt atattgccct tcaacgttca aaaactgccc gtgaagccat
     9061 tatggtcatt gccaatctga tgtcggaata tggttatgca tcatcgggcg aatcgttttc
     9121 cattgccgat aaaaacgaag catggattat ggaaattata ggaaaaggga attttggaaa
     9181 aggggcagtt tgggttgccc gtcgcattcc cgacggatat gtttcgggtc atgccaatca
     9241 ggcacgcatt cagacttttc ctttagccaa tggtaaaaca tccattaccg atgagcagtt
     9301 tgccaaaata agtcttccgg ccatcgaatg tgtgtattca catgatgtga tagattttgc
     9361 aagaaaaaat cttgaattta aaggtaaaga tgccgaattt agtttttccg atacatataa
     9421 tccaatatcc tttgatggag cccgtttttg cgaaaaccgc gtctggagct ttttccgtga
     9481 agtaaacagt gatatggaga aatatcttgg ttacattatg gggcatgatt taaagaaccg
     9541 catgcctctg tttatcttac caaataaaaa aatttcacag aaagatctga tgacttatat
     9601 gcgcaatcac atggagggaa cacctcttga tatgaccact gattttggtg ccggccctta
     9661 tgaatgtcct taccgctggc gtccgatggt ttgggaagtt gatggtaaaa cgtatgtgca
     9721 tgagcgtgtt actgcaacac agcaaacagg attcagtttt gtaactcaaa gtcgcaatat
     9781 ggccgatgaa gtgggtggaa taatctggtt ttcggtcgat gatgctgcca gtaccgttta
     9841 tactcctgta tatacaagca gccgtgaaat cccatctact tatgccagag gaaacggagc
     9901 aatgatggaa tggagtgatg atgcggcttt ctggattttc aaccaggtgt ctaattttgc
     9961 ttatacccgc tacaatgcca ttcatcccga aatcaaagtg aaacaggatt ctttggaaaa
    10021 tctgtatata aaacttgttc aggatataga taaaaaagcg ctcgaacttc tttcatccga
    10081 taaaactgca gccattatgt atgttactga tttttcggta aagaatgcgg atgggctggt
    10141 gcattgctgg aagaaatttt atcagtatct ttttatgaaa tatatggatg gaaatgtaaa
    10201 aacatatatt cccggtcagc agaatcccaa ggttgaatac cctggttatg gcgaaaaatg
    10261 gcttcggcat attgtgaaag ataatggcga ggagcttcag tatattggag ccccatcgca
    10321 ttaaataatg atgcttttaa aaatttgccc gaccttcctg ttttttcagg aaggtttttt
    10381 tgtgttttat ctacatattt tccgataact tatttacaat tgccgaatac ttggaccttg
    10441 tttctgttat tcatgtgaat gggtattttc gatgttggtt ttgaccggca aatttttttt
    10501 cccgcgaggt gaaaaaaacc accttataat aaaaattagt cgtatcttgc gccactattt
    10561 tcaagtagaa agttgaatta aaataaaccg accatagtat gaaaagaatc actactgttt
    10621 taggtgtctg tgtgttgctt gttttcttta gcagcagtgc actggcccag aaaaatgaag
    10681 gacagcaacg ctatcagccc agaaattcaa agggcaaaag cgagatgggt atctcgggtg
    10741 cactggaata ttacaagctt gttcgtggca ctcccgaaat cagcgatatt gtcaatgcac
    10801 ataaagctgc caatcagttg ccaaaaggca aagccatgac ctggtcagaa attggaccag
    10861 acaacattgc aggccgtaca cgggctctgt tgattgacag attcaataca ggtgttttgt
    10921 atgccggtgg tgtttccggt ggtttgtggc agtcattatc cggtggtact tcgtggcatc
    10981 tgattgctcc gttggctgag aattacgcag tttcatgcat tacccaggat atcaacggaa
    11041 tcatttattt tggtaccggt gaaggatttg gaaatggtaa tggtactgct gccggtagta
    11101 ctggttttgt tggtggtggg gtgtataaat ccaccgatgc aacaggtacc tctttttcag
    11161 ttatttccag cacggttcca acttctttaa ccagtgactg gatttttgtt tatgaaatgg
    11221 ccgccgctcc taacagtaca cgtatttatg cctgtaccga cggaggtttg tttattaccg
    11281 atgatggtgg tacaacatgg gttaatcctg taatttatcc gtcaaccact ccttatggtc
    11341 aggtctccca cgatgttgag gttgccccgg atggtagtgt ttatgctgtt gtcggagata
    11401 aagtgtttta ctctccaaac ggaaacagcg gaacttttgt cgatcgttcc attacaggaa
    11461 ccacggcttc ttctatcagc agggtcgaaa tagctgttgc tccttctgat cctaattaca
    11521 tttatgccag tgttgccaaa gttgatggct cgctgcaaaa tatttaccgt tctatcgatg
    11581 caggccagaa ctggacaatc attggccccg gtggcagcga tctttttcag gtctttggct
    11641 caaacaatca aggatactgg gataatatca tcaaggtttt ccccaacgat aaaaatcata
    11701 ttcttgttgg tggtgtcgat ttgtgggaat ggaccgatgg tggcacatgg ctgcagaaat
    11761 cactctggta tcttaacccc accagtgcat attatctgca tgccgatcat catgatattg
    11821 tttttcaccc gacaaattca agcatcctgt atcatgggtc tgatggcggt gtgagcaaat
    11881 caattgatgg tgcccagact ttttcgactc tcaaccgtcg ttttggtacg gttcagtttt
    11941 atcatttgag ctgtaatcct tttgatatgg taatgggtgg cactcaggat aatggcactt
    12001 tgattctgcc cggaaatctc acaagcaccg gtaccgccta cgaattcctt ggtggtgatg
    12061 gtggttggtc agcaatgtct tatatcgatc ccaatgtagt tgttggtact gtttactatg
    12121 gcgatatgta tcgttctgat gaattgggaa atagtcccaa agctttctac agcgaagcaa
    12181 tgaatgctcc tgaagaatta tcttcagcat gggcagggtt tgttactccc atgtatcttg
    12241 acgagcagat caatgatctg aatagccccg attcaatgta cttctttaat accgatacgg
    12301 tgtcaattcc tgctggtact tctgtagttg cttattcaaa gaaaaacaac aatttcccct
    12361 tctattatac aattgaaaat acgctggccg caggtgacag tgttctgatt caggataggg
    12421 ttcaggcgcg tttctatatt ggtcttaatg gagccgtatg gatgacaaaa ggcctgcatg
    12481 atttcagcgg tgctcctgca tggtacaagg ttgcaaatat ttccggtacc gcccagtgct
    12541 ttgccggttc caaagatgga aattatcttt atgttggaac caatatggga aatctgtatc
    12601 gcatctccaa tctttctgct gtgaaagatt cactgaccgg atatgttaca agtacacatt
    12661 ctgttgttga gtgcacccgt attgccagca ccagtcgtgc catcacttct atttctgttg
    12721 atccaagcaa tcctgaaaat attttataca ctttgggaat gtatgggaaa acggattatg
    12781 tttattattc aactaatgca ttggattcaa tacctacttt cgtgaataaa accggcaacc
    12841 tgcccaaaaa tcctgtttac gcaagtatca ttgaaatgag taactctgat ttggttattg
    12901 ttggaactga acttggtatt ttcagtacat ccgatattgg agcagcttca gtttcctggg
    12961 ttgaagataa cgaaactatc ggtagagttc ctgtgtattc tcttgttcag cagataaaga
    13021 actattacgg caccaataac tatggtgtta tttatgctgg tactcatggt cgtggtgcgt
    13081 ttaaatctac ttcttatctt ggtatcgaag atgagcagga agctacttca atggcaacca
    13141 ttggcattta tccgaatcct gctgaaacca cagctatttt gtcattcaat tcggatgaaa
    13201 aaggaagcgg tattgtaaat gtttatacca ttgatggcaa attggctgct tcgaaacagg
    13261 tgaaccttaa agccggaaac aatctggttg aactgaatgt ttctggtttg gaaagtggta
    13321 attatctcat cgaagttcgt accaacatgg cttctgtatc tggtaaactc attaaaaaat
    13381 aataagagag ccggggttcg ccccggcctc atttttaaat tgtaactttg aaaatctgcg
    13441 atatatgaaa aaatttctat ctgcattgtt catcctgatg gctgtgtcgg tatcagctca
    13501 ggaaaaaatt tatgcaccaa cactaacggc tccggctgat gcagctgtta atcagaaaat
    13561 agatgcttta ctcgactgga accctgttgc agctgcagta aaatatgaag taaatctcga
    13621 cacatcggcc agctttacca atccgcagga tgttgttgtt acttattctg cctggcagtc
    13681 atctttgctg ctctttggaa ccacatacta ctggcgagtc agagcaatcg atgccaacga
    13741 tgtaatttct tcctggtctg tggtacgtag ttttactacc tcggccgccc caagtcctct
    13801 ctggcctgct cccaattcac atgacgcgtc tctttgtcct caattgaaat ggactcttat
    13861 gtcaaacgtt accaagtacg aatgtcagtt cgattcatcg cttacctttg ccagtccctg
    13921 gttcaaatct gctcttgttg acagtacgaa ttacttccat gttttcggaa acgattatgg
    13981 cgaaaagtat tattaccggg ttcgcgcaat tcatatctat gatacatctg attggtgttc
    14041 acccgttgct ttttttaccc gtgactcggt cgctttccat cctacatcgc cgcttgatgc
    14101 cgatacggca attcatccgg ttgactctat cagagtcaga ggtatttacg gcaccaacag
    14161 ctacgaagta caattctcgg ttgattctgt tttcacagct ccacagattt tccattggga
    14221 cagtaccgca atgtggataa agttttcttc cggagcatat gataccatcg ctcgtggtcc
    14281 tatggatacc attccttttg gtacatttta ctacagggtt cgtttgatga ctgatgacga
    14341 taccaccaaa tggactgctg tccgttcact caccactgtg aaaaaggtta aaatgcttgc
    14401 gcctgctgat ggtgctactg ctgttgatcc tgctacatct ttctcttggg aagcaattcg
    14461 tggcgcagct tattatattc ttgaatatga tactgttccg gcttttacca atgtaaccaa
    14521 agttgatgtg gttggtacat catatactcc tgcagcacag cttttctcac aaaccaatta
    14581 ttactggagg gtacgttgtg ttaccaatgc tgacactaca gatttgtatg atgattttca
    14641 tttcaaaaca ttctggggtg taggcgttga ggaaaatgaa gtcgctacct ggagcgttta
    14701 tccaaatcca accagtggcg ttttttatct gaatctcgaa tcagcttctt ctgccagaat
    14761 cgaaatcctg aatcttcttg gtgatgttgt ttactctcag aatagtcttg ccaatggttc
    14821 caataccatt cacatagaca atctgaatga tggtatttac atgatgcgct tgtatgttga
    14881 tggtaaatta tatacatcgc gcgttgtaaa gaaatagaac aataatattt tttaaaaaag
    14941 ggctgttcag ttttgagcgg ccttttttta tgtatttgta ttatttgaaa tcatccatta
    15001 ccattgtgaa aaatatcata ggcggatgcc aaaggattac ttgacaaaca ggcaggtatc
    15061 acatgtttat aaaaatggca aaaatacacc ccccatgttt tgtcaggctc aacacctggc
    15121 cagcgggttg tctttttatg tttttcggga gacatagact catccggagt ctttcaaaaa
    15181 tgataacatc tattctgatt tgtcaaggcg gattaatacc aaagtgattt ataaatcggt
    15241 tcaattattt ttgggagact ccggaggagt catatatctg tagaccatat aaaacaaaac
    15301 cacgactccg gaggagtcgc atataaggag cttcgtcttc tgacagtgct tgttaatatg
    15361 cgacttctct gaggtcgttt gctcggtatt ggttttttta cagatatgcg atgcctgcct
    15421 tttcatcagg taaacctctg gcatcagcca acaaaacgtt caatgaaatt gctacaattg
    15481 actaaattat atttcctttt ggtataaaat aatttgagaa actgtatcca gaatgcaatt
    15541 attcaggatt gtccttcaga taaagcggat aaaaggtaaa agccggtatt agggtaaatt
    15601 gggtagttct ccaatgaaat tgagattttc ggtactggga tgtgttttca gtaaatcttt
    15661 ggtatttgta ttgagtttca ttggtcggct gcgacgatag gaataccggc ttgtgaaaag
    15721 gccaataccg tttgaaatat tcgaaaagct gggtttgtcc tgaatcagcg tgttggacgg
    15781 ctgattgaca tccatgtagg tgttaagatc ttcggcgccg atggtaatgg ataaaaatac
    15841 atatccggtg tatcgttgca catcatcgcg ttgttcgatt gcttgtccga gcaaatcgta
    15901 aaaagtccag ttgctgtagt tcatataaag cagttcgcca ccactggtat tgtcggattt
    15961 tacggttgca agattccagt caattgtttt ttcaatggta tcagtgggat tgctgtcgta
    16021 aatttcgtaa taatggaaag tgaatttacc ttcatatatt tttccgtttt taacggaact
    16081 gaatttcaca ttgctttgat tgtcggtata aaacgaaatg gtagtggaac tggagggtgg
    16141 tgtttcaatt aaaatattgc tcaggccggg agcttcgctt tcataaatgt taccggtaac
    16201 attatcggtt ataataagtt tgtagcgata atcagattta agccatgtgg tatcgtttgt
    16261 aaaagtgttg atggttgcca gtgcggctat attacttttg aagtcaaaac gataataggt
    16321 ttgatatccg gaaaaaaata cgccgccccc gtctttataa acattgacgg tgtcggctgt
    16381 caatggggtg ctcagaacac tttgcccgtt tgaattgacc acatccagtt tcacatccag
    16441 cagatagggg tacagggtgc tgtcgggaat ctgagcatat tccagcgcat ttccttcgcc
    16501 cagaaaagcc cggttgattt taatgtattg agtactggaa gctgcatcca ggatgccata
    16561 aacaaccatt acttcttttt tgtttccgtt gatatcaagt tcggtactgc atgaaaaaaa
    16621 agaatatgca gtggcggtca gaattaaaat tgaaaggact tttttcacag acttataatt
    16681 tgagacaatt gcaaaaataa ttaaaatgtt gcaaaatcaa tcattattga taagaatgag
    16741 ggcgttaaaa atcagtttat ggcctttttc agccaatggc tatttggctt tcttcgttga
    16801 acactgcatc agaaaaccgg agagactcgg ccaaaagatc tgtaattgtc attattttta
    16861 tttcaaggcc atgtttggcg gcgacacttt cgagttgcaa tacacagctg gtttctgtag
    16921 tgatgatata ttcagcgccg gctgcaaggg ctgttttcag ctcgtccaaa gctatttgag
    16981 cactcagttg tgaatacata accgaaaata cacctccaaa gccgcaatcc gatttatttt
    17041 ggtttgcacc caccaattgt aggccctcaa tcattttcat tagctgaagt ggttcttctc
    17101 tgagtttgta atcaattttt gcgctgcttt caacatgaag cattacttta gctttgaatt
    17161 tgcctttcca ggccgaaaca tgaagaatat tcatcagaaa atcggacaat tcaaaaactt
    17221 ttgtctgcag ctttttatat tcaagatgca atgatgtatt gtagaaaaag cggttgtaat
    17281 tgttttttac catgtgggcg catgcggccg agggcatgat aacatatctt tccgatacga
    17341 attcatgaat aaatttttca ccggcaaggc gggcctcatc ccaatgaccg gcattgtagg
    17401 caagtagccc gcaacaggtt tgttgttccg gatagtgtat ttcaactccc agatgacgca
    17461 acaccttata caggttctgt gcagtctctg gcttgaattg atcagtcaga catggtatga
    17521 agacacttac tttcatgctt gttttgcgaa aatcaaagtt acatattatt tttttcctgt
    17581 taaaagaatt tataaacggc cttgtttttg ttatatttgc tgttcaaaat aaaacgccta
    17641 tgaaaaagaa attacttttt tggtcagctc tcgctttgat tttcactttc acatcttgtg
    17701 atttattgca ggaagaaccg ccaacaaaaa cccagatgga aggtgtatgg ctggttacca
    17761 aggcctatga cctgaattca gcacaggata tcactcaaaa agtgaatttc ccgcttaccg
    17821 gctttcatct tagcagcgat ggaaccatta tatcaactgc cggtccactg atgatgtata
    17881 ttgtttatgg cgacagcaaa tttgtgaata ttgccagtga aattgaccag gtattcaatt
    17941 atgccagcct tgacttcaat ggtggtgaat tttttatagg cggtggtgtg cagacaaggt
    18001 ttactcttga aatgaaactt gaaggcctgc ccgggcaaaa agcactgaca accttgcttg
    18061 agctcatcgg tatcggaaat gattatcttg atgttgtggt ttatcacaag ttcatggatg
    18121 tgaaagtcga tttttccgaa gattatgaaa ccatgtactg ggaaatcgac aacatgacca
    18181 ctgctgttta caacacaaag gataattacg gcaattatgt tctctggcag ggatggcctg
    18241 taaacaattt ctcgaaagta cagatcactc ttcagaaaca ggtgaaagac ctcagggatc
    18301 tggttgaaga agcaagcaat taaaaatcag ttttctgatc aagcaatatt gaacccgctg
    18361 caatggcggg tttttttatt ttttctgttt atgaaaaaca aagatcagaa tgtgacctca
    18421 tacgaaaatg gtccataata cgaccaatac ggagaaatag cattgggcac aaatatgctc
    18481 ctgctgattt ctgcactttg aactccgtct ttgtaataaa accatttgaa aatactaatt
    18541 gctgaagcgt caacatgaaa atacacacac aaatccgatt gatttttaat aattctcttt
    18601 gtttgacagg ggctttgaat ttcaatgctg tcagcatcta ttatttgcca ggtgctgtca
    18661 gaaataaaca ttcggatttg accggttgga agaactttga tgatttgctc attatcagct
    18721 tctgatgcag aaatcacggg tctatcatca gacagatatt tgaattcgct taaagtggaa
    18781 tcaaaagtat tgggcgaatt tgatgaaaga ataattttat caataaaatc acctctttga
    18841 gtataatcaa attcaattgt gaaatttccg taatagtccg aatgtgtact atcaagatac
    18901 cccccacatg aagaaaccca tgactgaagg gaagcaccaa caaaggctcc gcttacaggt
    18961 agccctgtat aactgtttat tactttgcca tgaatactaa aatgataagt ctcttttttc
    19021 tcgcaaccgc agaaaatcag agctatgaca accgaaagaa gctgaatttt ccttttcata
    19081 ttgtatcttt tagtatctac cttatcagtg ttatatgtcc ccgttttgta aaatgctggt
    19141 cacccggcag agtcatctga agaatgtagt ggtaaacgcc cacaagtgct ggtgcgccat
    19201 cgttggtgcc atcccagcat tcgtaaaaat cagtggtgtt gaacatctct tcgcccagtt
    19261 cattgaagat gataaaagag taagtggtgt ggtcgatata cacaccaaag gggcagaatg
    19321 tcttattgta tcctgtcggt gaaaaagcat ttgcaatgaa cagtcttggc aattgctgaa
    19381 tttctatctg gttggataat gttgtgtcgc tgtacccaag ctgatttccg gctgactcaa
    19441 tgccttcaag ccgataggtg aacaaaccgt agctcagata aaatgatgca acatcatcaa
    19501 tatgatattc agcaccgggt gtgaaagtcg ctatcggtgc ggcatccggc acattgtcga
    19561 tcatccggta caggcgatat tcctgaactc ctgttggcca gccctcgtaa tcattccaga
    19621 aaagttcatt ggtgagatcg ctcaatggtt ctcccgaaag aaaaatggta cgtgcagtgt
    19681 cactgtacat ggcttcgttg ctgcaggaat caattacagc tacccggtag taaaccgaat
    19741 gcaaatctgc actggctgtt gcatcggtga aggtatattc gggcgtggcc gtataagact
    19801 gataatcgat cgaagtccac ggaccatctg gtgtcagact gcgttgcagc gaatatccga
    19861 tcacatacac gttgcggtca tttacccatt tcagatacac gctaccatcc gtttctactg
    19921 atgcatgccg gatgtacaca taggaaggca gctcaggaaa atcaacaaac tggcatgaat
    19981 tattggatga agaaaagaag ccattggttg aaactgcagt tacataaaag cagtatgagc
    20041 tgtcatcttc cagattcaga acgtcgtacg tggtcgtagt atttgacacc acatcgagga
    20101 cagaccaagt gccaccgttt tcagaaaaat ataagacata ttcatcaatg ccgggaaagc
    20161 cgctataggc cgtccaatcg agatttacgc tgcggctgca gggatcaacg gtgctgctca
    20221 gaaaaatggt tgaatggccc gttgtgagcg gacttgtggt tgcacaggaa tcgatggcag
    20281 caatgcagta tgattcggaa tttgcatcag cagttgatgc ggcattttcg tacgatgtgg
    20341 tgttgatgcc ccagacagta tcaatgggtg tccagacgct ggtgaaaata taaatgatat
    20401 agccctcggt atcttcggat gctgaaggct gccatccgag aatggcattt tgcgttgttg
    20461 ggttcaccga aacgctgtcg aatacaggaa tttcgggtgg tatagtattt tggtaaatgt
    20521 cgccatcaat agaagaaacc gacatgcagc cggaagcatc ggtttgttga atacgaaaat
    20581 ttatctggct gttgcagata tcaatggtgt cgataaaaga agtggtcatt gatgttccgg
    20641 cagcagtcca tatgccggcg ggaaattcgt agaaaatatt gtaattggcg gaagtaatca
    20701 aaggcggcat gtggagcgcg ttccagctga gcggagctgt tccgttcagg ggattcagag
    20761 cagtaagcat catggtctgc agagtgtcgg atggtgttga atattgcaat gccggcgcat
    20821 cggaaacagt aatcatataa taaaaaccag cattaatctg tgcgttggca ccagcatgca
    20881 accaggtgtt ttgtgtgtaa agcgaaacat ttccaaccat tatgtaaggc cctgtcaggg
    20941 tggtggaatg aaaaatctga taagaatcga aaacagcagt tggatcgggt ggaataaccc
    21001 aggaaagctc tacatcgccg tttgatttca catcgaggca tcgcagcgaa ggcgcattga
    21061 gggcggcgaa agaaatagtt ccccaaagaa tgagtacagg gaaaaatatt tgtttcagcg
    21121 gcactttcat tgattaattt ttattaccga caatcagaac gcgcatttca tcaggattca
    21181 aatatttttt tgccattcgc tgaatgtcat cgggatttgc tttggcaatt gtatcccaat
    21241 aatttctgaa ataatctttt ggattgagtt gttcggcgtg catggtcatc agtcgttcgg
    21301 tcagcgcaaa ggggccgtcc atgctgcgaa gcagacttcc gaaaatataa ttacgtgcaa
    21361 tattcaattc ttccatttga actttttcag aagataaacg tgatatttct ctgaaaattt
    21421 catcgagggc gagctgagta gtttctgatt tcacttcggt ttgaatggac agatggctgg
    21481 ctttgggacg aacagcgatt ccggatgaaa ttccataggt ataacctttt tcttcgcgta
    21541 tggatttcat tagccggctg ccgaaataac caccaagaat ggtattggta agcatgagca
    21601 agggaaagtc ggggtggctt cgaccaacaa cgggtgtgcc aagcataatg gcagactgca
    21661 cggcatcttt tatttcaata attctttgtt tttcggaaga gggttgaatg tcgaaattct
    21721 tttcatcggt cgtaatcaga tgcttttttg tttgttccat catttcagat aatgatgcaa
    21781 tgacatcggt atccatattg cctccaatat aacagcgtgc attgcctaat ggaagctggc
    21841 tgtacaattg tttcaaatca tcgcttgaaa tattgtcgaa gtccgattct ttcagaattt
    21901 ttccgtaagg atgggtttcg ccataaagca tggaaggcag tgtacgtcgg gcaatgctca
    21961 agactttttt gttgccgatg atgaattcct gtttattgtt ggcaagaatc accctgaatt
    22021 cttcttccgg aaaaacggat tcagtcatga tggaaatcaa caacggaaaa agccggacaa
    22081 gaatatgttt tactgcaaaa atactgatgg ttgtgaaatg atttgaggcc gtaatatcga
    22141 tgtatgcgcc gttgaaatca atggtttcgg caatctgaaa ggcattgtat ttttttgttc
    22201 cttccatcat cattcgcgcc atggaagatg cctgtagtgg tttttgctga gaccatgtgc
    22261 cagcgcctat cacaatgtcg agacgaacaa cttccgaatt gaagttttcg aaagaatagc
    22321 agggaatcag attgtttatt tttgtttccg agaaatcggg aaatccgctg aattggagca
    22381 atccggctga tggagcttgt tttcggttga tattttgatt gttcatataa tctgttttga
    22441 cactgggaaa aggaatttgt tatttagccg gttcataatt tagttgggaa aggttttctt
    22501 tacggaataa ttcattagct acacggcaaa tatcgtcttt agtgatggct ttgtaatgtt
    22561 ccacttcgtg attgatttgg tttgcatcgc ccatccattc atataaagca agattcattg
    22621 cattattgag acaactggtt ttagaaaatt caaaagtaga ttcgatttta ttctttactt
    22681 tttcaatttc gcggtcatgg atatttcctt tttttatttc ttcaatttct ttatctatgg
    22741 ctgggattac atcatcgaac gaataaccgt tggagagttc gccttgcaca ataaagagtc
    22801 ccggatcaac atcgccggaa atgtaggcat tcagactgga gaaatagttt ttatctttta
    22861 cgagattttg atacaacctt gatgaggcac catttgagag cagatcagaa atcatgtcac
    22921 aaactttata atcaggatga tttcgtgcgg gcatgtggta ggcctgatat agcgctttga
    22981 atggcacatc gcgggtaaga gtgagttctc tttttttgtt ttgcactggt tcctgaggta
    23041 tctgatgtgt agctgtatga ggtttatcga tatttccgaa ccatttttca gcgagacttt
    23101 tcaccacttt caattcaagc gggcctgcga caaccataat agcgttggca gggttgtacc
    23161 atttgttgta gaattcccgc acatcgctca tggtcgcatt ttcgatatgg ctgatgtctt
    23221 tcccgatggt tgcccatttg tatggatgcg tggtaaaagc cagtggtctc agatagagcc
    23281 aggcatcgcc ataaggctga ttcaggtatc gttgcttgaa ttcttcaatg acaaccttcc
    23341 gctgcacttc gagcgacttt tcggaaaagg ccagactcag catgcggtcc gattcgagcc
    23401 agaatgcagt ttcgatgttt tgagccggta aagtaatgtg atagtttgtt atatcgttat
    23461 tagtgaaagc attgttttcg ccacctactt tttcaagtgg tgtatcaaat tcaggaatat
    23521 ttatacttcc accaaacatg agatgttcga acaaatgggc aaaaccggtt ttgttttcat
    23581 cttcatcgcg ggctcctact ccatagagta tgttaatgac cgccatggga gtggttttat
    23641 cgttgtaaac caggactttt aatccattgg aaagcgtgaa tttttcaaat tgaagcattg
    23701 aaattatttt acttcaaagt taaataaata tctttgacaa aaaaaacaca tgctccggga
    23761 tgataatgtt gtgcggaaac cggctctgta tctgaaaagg ctcatcagac acaagcaatt
    23821 tgtgttgctg ctcagtatta tggttgggtt ggcggcaggt cttgctgcgg ttattctgaa
    23881 aacttctgta cattacattg aggcctatgt ccgtgatcag gccaatgtgg atgccgaaaa
    23941 tttctattat ctggttttac ctgttctggg aattctgatt acggtttttt tcacggtgta
    24001 tattgtaaag gacgatatca gtcatggagt atcaaagata ctgcgagctt tatcgaaagg
    24061 cgagtcacgg atgaagccac attttatgta ttccgggctt gtaggttgtt cggttacagc
    24121 cggaatgggt ggttctgtag ggatggaggc cccgattctg gcaacaggtg cggccatggg
    24181 atccaatatt ggcgaatggt tcgggcaatc gcaccgcacc cgaatgctgc tcatcggcgc
    24241 aggcacagct ggtgcattgg cagccatttt caaggcaccc attgccggtg ttatctttgc
    24301 aatagaagtg ttggctctcg aagtttcagc tgcatctatt atccctttgc ttatcagcgc
    24361 tgttactggt gccctgcttt ctactttcat gcttggaaaa aacattgagt tctatttttc
    24421 cattcaggaa ccgtttgaat acagaaatat tttttattat gttattctgg gtattgtctg
    24481 cggtcttgtt tcagtgtatt ttctacgcac caatagctgg attgaagggg ctgttcaaaa
    24541 aataaggaaa aaaatctggc gtattgttat aggtggaggc gctgttggat tgctcatatt
    24601 tctgtttccg ccactgtacg gcgagggtta tgcttttatg cgcatgattc tgagcggaaa
    24661 tgttcatgat ttatcaaaca attctttctt ttataacttt gatgaaaatt catggatttt
    24721 tgttggatac cttggattga tcctggtctt gaaagttgct gccatgtcgc tgacaactgg
    24781 gtctggtgga atcggcggag cttttgcacc atctttgttt atggggggga taaccggagc
    24841 agctttcgtt aaagttgtca atttgctcgg tttcacaacc atcattcaat caaattttat
    24901 tcttgttgga atggctggtt taattgctgg agttatatcg gctccattga cagccatatt
    24961 tcttattgca gaaattaccg gtgggtatgc cttgtttatt ccactgattg taacatcaag
    25021 tattgcgtat atgacaacgc ggctctggga gccctattcc atttatacgt cccgattggc
    25081 tgtgagcggt gaactggtta cacatgatcg cgataaatcg gcattaacac tgcttagagt
    25141 gaatgaagtg gttgaaactg atttcaaagt agttcatccc gatcagactt tgcgcgattt
    25201 ggtgcaagtg atttcggctt caaaccgcaa cctttatccg gttgttgatg aatactgcaa
    25261 ttatcttggt gtgattacgc ttgattctgt aaggaaactg atgtttgacc aatcgaagta
    25321 cgacactgtt ttgttgcgta atctgatgca gaaagcaaag gaatctgtat cactcaatga
    25381 taatcttgac acagccatgg gcaaattccg caaaacaggc tggtacaatc tgcctgtgct
    25441 gaatggcgag aaatatgtcg gatttgtttc gcgctcaaat attttaatga tctaccggaa
    25501 taaaatccgt gagttttctg ctgagtgata aaactcctcg aaggg
//
LOCUS       DAOPKE010000035        24712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2129_length_24712_cov_13.431358, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000035 DAOPKE010000000
VERSION     DAOPKE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_24712_cov_13.431358"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1521)
                     /locus_tag="PKY63_06265"
     CDS             complement(133..1521)
                     /locus_tag="PKY63_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00253.1"
                     /translation="MTQFLSPEKYIRQRARTLPIHECLVNDEWNQNQLATVFIVRKHV
                     NGHFTIGNYLVDLKCLGVKDTFFWFNVSADDYEDLKDDLFSKQEMSVISYELAHNIVF
                     AGENFATEIGLKSHPDFYKTTQFILEEDNDNIELIDIEVGDNGVPVFVQGPFDTPDRV
                     YEILETLNKTVGNGNYHYVDVSELDSEIDSDNDDDNDNDDNNNFEKYFAYSFNDLKNL
                     LLNFETIAVTNFGEHSLIGITTSLIYRIEGDRKIDNLFAQLSELLDFKVDVEEVHPSL
                     FGLASFDDRFNKEELTNDFVDACRNLSEKKVGKKLVAAFGKKYPENPAYNYLLAQTEF
                     AKDKNNSSSAVQKYALSCPDYPLIQLLFSVASLLNTPADDNLDFPEFYSAWFGNRKVL
                     HPIEATDYIFYLSTFVFKKENLAWGLAFIEALEESDIPVEIVHYYRTIGFLSLVIILR
                     DRLSTEDFSHLK"
     gene            1752..3914
                     /locus_tag="PKY63_06270"
     CDS             1752..3914
                     /locus_tag="PKY63_06270"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPF00254.1"
                     /translation="MKIKALFLSFVLFISISLRTLADEGMWLPLLLQSLNEKEMQSMG
                     FRLTAEDIYSINQGSMKDAIVLFGRGCTAELISDQGLILTNHHCGYGQIQSHSTVEHD
                     YLTDGFWAMSKEQELSCPGLTATILVRMEDVTSKVRAKLTDDMTEAQREDAIKDVSDK
                     IARDAVSGTHYQAQVKSFYYGNEFYLMVYEVFSDVRLVGAPPSSIGKFGGDTDNWMWP
                     RHTGDFSMFRIYANKDNKPAAYSKSNIPYKPNYVLPISLKGVKEDDFTFVFGYPGTTE
                     HFTISDGVKIITEEENPVAISIRRTRMDVMEKYMKTDPAIRIMYASKHAGVGNYWKKM
                     IGESKGIRQWNGISRKQAQENDFMETLRHDKEMFTKYGTLLENMKAAYENLRPYSLAY
                     TYFFEGAYSIEALKYAYSFTAYINECIKNPKSDLIPGAKDKLLKSVDSYYKGYHADID
                     KEIAILLMPILKNMGPRFLPEAYTNMESKWKGDWKAAMDDAFKRSFFVSKEKTLQFLN
                     MNPQKVKKAIQKDPVYQLTSGFFNSYFDKIDPGQSLYRAQIDSMYRIYVGIKKDMKTS
                     ERMYPDANSTLRIAYGKVKGFQAADGVNYKYYTTVDGILEKESPEIEDYYVSPRLKEL
                     VLAKDFGIYADADGTLHTGFIATNHTTGGNSGSPVLNANGELIGINFDRVWEGTMSDL
                     MFDPGKCRNVSLDIRYCLWVIDKYAGAGYLIEEMKIVK"
     gene            4137..5198
                     /locus_tag="PKY63_06275"
     CDS             4137..5198
                     /locus_tag="PKY63_06275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPF00255.1"
                     /translation="MQTNPNIFIDCNRAKANLKAVFEDQRFSDVTFRPHFKTHQSIGA
                     GMWFSNFGIEKITVSSLPMAEYFAAGEWDDILIAMPLNPNHIAQYNTLASKIKLHLLV
                     DNNDTLSIALSEIKNPVSFYLKADAGYGRAGWPAENTKEFLKALKLISKNGTHSFSGI
                     LSHFGNTYSARSVEEIVDINLSSITRLVVLKEYIETQTGKSCPLSIGDTPSLPQYTPE
                     MLKNISELRPGNFVYYDMMQVALGTCQINQVAAALKCDILSVYHDRNEVLVHAGAVHL
                     SKEVCPMPDGKPGFGALVRLNEGNICEVIDDCFVDRLSQEHGILRVTPAFFNEFGTGD
                     SIGILPVHSCLMVAADSRQ"
     gene            5780..6895
                     /locus_tag="PKY63_06280"
     CDS             5780..6895
                     /locus_tag="PKY63_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00256.1"
                     /translation="MKKNLIFACMIMLVSCFSCQKDIESIKPSSIQPTLMIKSIEDYK
                     PEPNKFMPLIEKVKQYSGETNNGNIKTQENDSILIQDAFWQLETAINYYYGITGAPKD
                     TIIEDSLILNLPFSIEGSEGYVMIPDVASLYSEMKEEILTYFPQHILLYGDLTANEVE
                     GNILKMVFNFTMSNPPSDLISGNGNKDLIFLDQTYNCSTCRVPVAIDPYTAIVTPFNS
                     NESYPAIFAQSGTPGTLTFTGSAREKIQNKLNGSANAIPATPGFKIVLSNYSNNNFFT
                     YNGSYNLYINPGSLFGVQSNPCEILNSVSLNIYLQKAIQMMQFASPAAPRKIICAKYS
                     WVGPTDDGIPDPYPAWSWIFICPTAEVTYVPINQGTN"
     gene            6898..8349
                     /locus_tag="PKY63_06285"
     CDS             6898..8349
                     /locus_tag="PKY63_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00257.1"
                     /translation="MKSLLFVLFLLACTLLKGQGFEKIRFGSPDTAYVGVFTDELTEG
                     KYTVLCGQYVDHIPVGGEILEYDVSGSLGKYVKLPEYTGLILEPERIIRSGINQLILA
                     GNAWNNTNDEQDLFLMTLDNSYQIQSIVIVNNPTLDDRFLNWHFFNDSIVDIACKDLD
                     SAYYNSYVLRVRLNGQIIKQTTPGYPVNPVDFYYKKSDSTYYIINSDVIFGFSADSLN
                     PLGILFDQFVYNQSHNYPVLQTGNAFGINDSVFLTMGHYMDFSGTIPIREMGISLWNN
                     SFDPVIIFHNGEDGYYNQSPSNSFSFIDPKHAFLSGYSLLLPDGIKQQTTLDEYRILV
                     FNINTETGAENWRRLFYSNYCAFPDFTSSTSDGGCLITGSRTDLNGIRTDMFLLKLDS
                     LGNCTPDVISENQKPAYEIFPNPVSTYMRVSSQGSIISFSISDLSGRSLVNEKCYGNN
                     DILIETEMLDPGIYLLKASFNDGSTAVKKFLRL"
     gene            9007..9927
                     /locus_tag="PKY63_06290"
     CDS             9007..9927
                     /locus_tag="PKY63_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPF00258.1"
                     /translation="MSIPTTRTKEDAVISINVEICTGCGLCVKACCSDMLKIENGKAA
                     ELQGKSVFNCIACGHCMAICPEGAIKISGRTMSPGDVFDIPKDASDATYEQLMTLFER
                     RRSVRKFNQKTIEPEVIEKILAAARTAPMGLPPSDVHVLILNGREKVTAFTRDFAQYL
                     KSLKYLVSGWFLAIMRPFWGKTNDEMFRNFIRPLFENYIGEMENGNNYITYDAPLAMY
                     FYGSPYSDPADPIVAATYAMHAGESLGLGTCMLGAIHPFIQRGKKAAQFRKAHGIKYK
                     SQEGLFVIFGYHDLKYKKGIKRTFADETFA"
     gene            9911..10672
                     /gene="bla"
                     /locus_tag="PKY63_06295"
     CDS             9911..10672
                     /gene="bla"
                     /locus_tag="PKY63_06295"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HPF00259.1"
                     /translation="MKLLHRKYFIVLLLLFPVCASGQHSDTIRINKDIQLIQLKDSFF
                     LHVTTDYVEGFGMVSSNGLIVIRNGNSLMIDTPMDKIKTSAILDYLRDSMYTTVTVFI
                     PGHWHNDCIGGLAELHRRNVFSIANEMTRAECIKRNLEVPKASFSKYLTWSFCGIPLE
                     CFYPGAGHSLDNIVVYFPEQKILFGGCLIKSAESTSIGNIADGDVEAWPKTLKKVQKK
                     YPDAEIIIPGHGSVGSLKLIDHTIFIINHYLNSKK"
     gene            10761..12056
                     /locus_tag="PKY63_06300"
     CDS             10761..12056
                     /locus_tag="PKY63_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00260.1"
                     /translation="MKHYIFIFIACSYLSGVAQQGKPCKDISFKTPQKATLETDNGYR
                     ADSCEYHCLCEPQSIDGEKHYYFYDHLGRDTSIWNFYQDTIINPWYLESKNTKEYTSF
                     NAIQNEKGYWYDTLGMWQYTFLRQNTFNTSQEMIHQKNYRLYESTLPHSISDFTYGPN
                     GLSQSLYFVAYPTYPATLRSKQTYFYNVSGNLSYIEYYSYNTQTSTWSPTVSYFYTYD
                     SLENLIQYSRFNYASGAPLCDYKEQYEYDGNGNMTLKTISTIEYGNPSLQITRTIETF
                     FNTFNKPDSAITSEYYMGTSFDGKLEKYYYDSLCNQIRWEYYLHWQGNSSWQLFGWED
                     CYYSQHIPTHGISEPATNLGFTVFPNPASDIIRVSSEEAEFSEITIMDLRGVELMRLN
                     AEQSNQVSANISALSKGIYFIRCTAEDGKSGIKTIVVQR"
     gene            complement(12060..12878)
                     /locus_tag="PKY63_06305"
     CDS             complement(12060..12878)
                     /locus_tag="PKY63_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPF00261.1"
                     /translation="MSFLDWFESIGHVGQAAIATTFTWGMTALGAATVFLFREINRKL
                     LDVMLGLAAGVMIAASFWSLLSPAIEMEEARGGIGWIPAVIGFLAGAVFLRLADRLLP
                     HLHSGMNKSEAEGIPVKWNRNTLLVLAITLHNIPEGLAVGVVFGSLASGIEGTTLAGA
                     IALTIGMGLQNFPEGAAVSIPMFRDGRTRMKSFMAGQFSGMVELAGGILGALAVLHMT
                     TMLPYALAFAAGAMIYVVVEELIPESQRGKHNDLATMGTMAGFALMMFLDVALG"
     gene            complement(12878..13588)
                     /locus_tag="PKY63_06310"
     CDS             complement(12878..13588)
                     /locus_tag="PKY63_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00262.1"
                     /translation="MKPELIQELFEKHGGVFMTPAPKIAKKLENIHGFLFDWDGVFNS
                     GKKSSEFPSTYSETDSLGINLLRFSYWLRYKKIPFVGLVTNQITESAFDLAKREHYNA
                     VYVNFKSKKEAFTHMNDTFNLRPTQVAFIMDDVLDLSASDVAGLSFMVGRKSSPLFMN
                     YVHEKDLVDYISYQRGDLNAVRELCELVMGLTDIFEETIQQRVAVSDEFQAFLEQRDE
                     IESKFYHKVDRKIIETVI"
     gene            complement(13631..14911)
                     /locus_tag="PKY63_06315"
     CDS             complement(13631..14911)
                     /locus_tag="PKY63_06315"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPF00263.1"
                     /translation="MQSAVRVFKFGGASVKDAAAVRNVASILKKYDNQNLVVILSAMG
                     KTTNALERVHQAYVSGGKSDALKRVSESKQFHDSILNELFKCDHAVFNAVSDLFSALE
                     NELDIAPESSFDKSYDRIVSFGERIATTILSAFLLDAGFHVTHIAAGDYIIADNTYRD
                     AYVNWEETGARISGAGQSWFAKTGIVLTQGFIGRSEEGCPITLGREGSDFSASIFAHG
                     LNAAEVIIWKDVPGIMNADPKLISSAVSLPHISYREAVELAYYGATVIHPKTIKPLEN
                     KNIPLSVKSFVDPAGTGTTIDRNGSDDKSVASYIFKFNQVLLSIFPRDFSFINEKNMS
                     EIFTMFSRNNIKVRTMQNSAVSFSVCFDFEPAKLSVLIEDLQKRFLVRYNDSVDLVTI
                     RHYSSNSADEFIAGRKVLLEQKSRSTWQLIISKA"
     gene            15022..15660
                     /gene="udk"
                     /locus_tag="PKY63_06320"
     CDS             15022..15660
                     /gene="udk"
                     /locus_tag="PKY63_06320"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPF00264.1"
                     /translation="MLVIGIAGGSGSGKTTVVKAVMQQFTKGEVCLLSQDSYYRDNGH
                     LSAEERARINFDHPSSIEFSLLCKHLDALKAGEDIQMPIYSYVTCARSKETIPVSPAR
                     VMIVEGILVLANPRLRDRLDIKVFVDADGDDRLMRIIRRDIEERGRSFLQVLEHYERF
                     VKPMHQTFIEPTKRYADIIIPQGGANKIAIDILTAKIGSKLAEYNGSLSSSQ"
     gene            15635..16273
                     /gene="aat"
                     /locus_tag="PKY63_06325"
     CDS             15635..16273
                     /gene="aat"
                     /locus_tag="PKY63_06325"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPF00265.1"
                     /translation="MAVYRLPNEIVFPDPHEAEPDGLLAVGGDYSVERLLLAYNHGIF
                     PWFQYKGDIYWYATNPRMIVFPDKYRPNHGLRRVLKKCPYRVTINTCFEEVLNQCAKI
                     KRKEKGTWINKKYKKAFIELHEKGYAISIETWLGDELVGGLYGIVAGSGFTGESMFSA
                     HPNASKVAFHQLVMLARHLKWKFIDAQQDTPHMRTLGGELISFDDFYLLLTE"
     gene            16357..17958
                     /locus_tag="PKY63_06330"
     CDS             16357..17958
                     /locus_tag="PKY63_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF00266.1"
                     /translation="MKYTIPYLFSESVKKNSECPALTFVGDKPLTYSDTAKHICNTIG
                     FLQNRDISKGDKVALWGTNSPQWAIAYLAITSMGAVVVPILPETLPADVVNILNHSES
                     KLIFLTQPLAVKLSEEKVSDSVIQINLEEHFDEAPETQTTCDFDVFDVQENDLAAIIY
                     TSGTTGRAKGVMLTHRNISHVGWHSNDIQSINNQDRFLSVLPLAHTYENLIGFLLAMF
                     FGACVYYLRKAPTASVLMPAMTEVKPTLMLTVPLIIEKVYRQKIIRTFSKNRLIRFIY
                     SIPVFRKLLNRIAGKKLYASFGGHLKFFGIGGAKLNASVEKFLREAKFPYAIGYGLTE
                     SAPLIAGANAQYTRYQSTGPAYTGISIKINNPNPETGEGEIWAKGPNVMQGYYKEPDL
                     TAEVLTTDGWLKTGDLGKLSKDGYLYILGRSKNVIIGASGKNIYPEEIEALINNYPLV
                     AESLVLERKGRLVALVQLNMEDLEEQIAELKSNIEARKEEILEDIRKYVNERVSQLSQ
                     LQSVMFQPTPFEKTATLKIKRYLYA"
     gene            18187..18735
                     /locus_tag="PKY63_06335"
     CDS             18187..18735
                     /locus_tag="PKY63_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00267.1"
                     /translation="MAAPKIIIVTENLQELKQILKSTSAFLAPRVRMLIELKRHENSG
                     ISKRDLAELIGVNHNSVQTWRTAYEKGGINLLLSHKKTGYKPSVITKEEHRIIEKKLN
                     DPTNNIRGYKELQQWIEDELEKKIVYSTLMKYCVRKFGSSIKVGRKSHINKDVEAVEG
                     FKKNSRKSASRQSKAKKYDLKK"
     gene            18702..19241
                     /locus_tag="PKY63_06340"
     CDS             18702..19241
                     /locus_tag="PKY63_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPF00268.1"
                     /translation="MESKKVRFEKVNLFFQDESRFGLFTRNGKALTAKAVKPIIPFHQ
                     VFKSLYLYGAFSPINGDGFLLELPACNADLFQLFLNEFSLQNPDELKLIVLDNGAFHK
                     AQKLAIPENIVLIFLPPYSPELNPAEKIWAKFKRDFTNRLFHTLDSLEKYLCELSSSL
                     QRSEVISITSFSYVFSCPL"
     gene            complement(19255..19557)
                     /locus_tag="PKY63_06345"
     CDS             complement(19255..19557)
                     /locus_tag="PKY63_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00269.1"
                     /translation="MNFKTRTAIIISISILLISAIAFFAFDKIGHAQNKLNTAADIIE
                     SIGNSSFMVEADVKDTIPVKTSISIPISIPVNVSLNMALDVPLSMNVPINKNLISK"
     gene            complement(19557..20045)
                     /locus_tag="PKY63_06350"
     CDS             complement(19557..20045)
                     /locus_tag="PKY63_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00270.1"
                     /translation="MKLFPHFPKMSFALIIICFLLPFVTVKCGNYEIAKMNGIHLATG
                     TQIEADSDEEPYNINPDIFIISSMILAVAGLIMAFLKFRGNTIASLIVSVLGLAALVY
                     FYFHIRKDIPSEGSMVIIISMGTGYYLTTIGFLLNSLFFGYQLSLKKEIFEAETRNIE
                     PE"
     gene            complement(20184..21185)
                     /locus_tag="PKY63_06355"
     CDS             complement(20184..21185)
                     /locus_tag="PKY63_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPF00271.1"
                     /translation="MIKIAHKIGIVGGGSWATAIIKVLQHHQKKVNWFIRRQEVIDAI
                     RNSRHNPEYLSQVELDPTKIVASNNLDEVISNSDIVIFCIPAAFLHQQLESLDKKIFQ
                     DKIIISAIKGVIPEFNSIISRYFNTVFDVPAENYGMIAGPTHAEEVVMDRLSYLTAAS
                     VNEDIGTVLTELLGSRNIKISVSSDVVGIEYASVLKNIYAVAAGISVGLGYGDNFLSV
                     LTTNAIMEMRGFLDAINHCKRDVNMTAYCGDIIVTSYSQFSRNRMFGNLIGKGYSVNY
                     SRIEMKQIAEGYYASKCMSEINEEYKVDMPILNAVYNICYNNVSPSQEMRRLAEKLK"
     gene            complement(21192..22868)
                     /locus_tag="PKY63_06360"
     CDS             complement(21192..22868)
                     /locus_tag="PKY63_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF00272.1"
                     /translation="MHRFFILSLLLLQSLFLQGQFLQNISAEEKHAMADRLYNHVGAL
                     AHDSMLGREAGTEGEAKAMQYIALEFSNTGLKPLPEKDYCIPFQFSEGYSYTSGTVYI
                     NEKPFRLNQDFFYINPLVNYTFTGKIFFAEDGLSDEKYKELNPTFNKNVVIIIDINYS
                     DSCSNSQSQSYTGLLETAVGKATRFNPESVILVSSNNENFPVTAAFNTDRNRANIPVI
                     GAGKDLSKTILKNKTANLRSECIFSKNEKTGYNVAAWINNNKPTTIVIGAHYDHLGYG
                     SAGSLSNDSAIHNGADDNASGVAVLIELAQQINKIESPQHNFLFIAFSAEEKGLLGSK
                     AFINQNHLPDSSFVAMLNFDMVGRMDKENPKLNIIGTGTAAEWDSLIEQSYAGTINIR
                     KSASGTAGSDQMSFYLKDIPVLFFITGMHEDYHKPSDDTEKINRDGMADVLLIALEIT
                     EKLNKREKLHFQKTESASGNRGKRPGVTLGVIPDHAWEGKGMRIDAVTKGKTADQAGM
                     LSGDIVIQIGESTVTDIMSYMKALSAFKKGDKTKITYLRDQIENSVEVVF"
     gene            complement(22880..24532)
                     /locus_tag="PKY63_06365"
     CDS             complement(22880..24532)
                     /locus_tag="PKY63_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPF00273.1"
                     /translation="MKRTALILSVLLSAAVLTSCGSDKKKTDENKDSTGVTMKDKVDQ
                     YAMVELTANISHLTEKEKQIIPILFEVADIMDGLYWMQTLGDKNAFLDTIKDPDTKRF
                     AEINYGPWDRLGDNKPFVNGIGEKPLGATFYPADMTKEEFEKLADKNKTSLYTVIQRN
                     EDKTLKVVWYHEMFKDQLQKASDLLLKAAALAEDAGFKKYLELRAKALITSDYFPSDM
                     AWMDMKTSNIDFVVGPIENYEDALFGYKAAFESFILIKDIEWSKKLERFAKLLPELQK
                     QLPVDEKYKKETPGADSDMNVYSAVYYAGDCNAGSKTIAINLPNDEKVQLSKGTRKLQ
                     LKNAMQAKFDKILLPIANILMDPSQRQYVKFDAFFHNVMFHEVAHGLGIKNTINGKGG
                     VREVLQDMYSSIEEGKADITGLYLVTKLREMGEITEGDIKENYVSFVAGIFRSVRFGA
                     ASAHGKANMMCFNYFMKNGAISRNDKGFYVVDFEKMPSVVESLVNKILVIQGEGDYAA
                     AKKWVETDGIIPADLQKDLDKLEENKIPVDIVFKQGKSNLGL"
BASE COUNT         7466 a         5172 c         4983 g         7091 t
ORIGIN      
        1 tgtaaatggc tgcctgtgcg aaacagcttg aaaaacattc gactccgctg gagtcatcca
       61 aatccatgaa ctattaaata tttttttggt attaccccaa aacctgaaca aatcagtgca
      121 taaaaaacgg aattacttca ggtgcgaaaa atcttcagtt gacagtcggt ctcgcagaat
      181 tataacgagt gacaaaaatc caattgttct ataataatga actatttcaa ccgggatgtc
      241 tgattcttca agagcttcga tgaaagccaa gccccatgcc agattttctt ttttgaaaac
      301 aaacgtactc agatagaaaa tataatcagt tgcctcgatt ggatgaagca ctttccgatt
      361 accgaaccat gcagaataaa attccggaaa atctaagtta tcatctgcag gagtgtttaa
      421 taatgaggca acagaaaaca acaattgaat cagcggataa tcaggacagc tgagcgcata
      481 tttctgaaca gctgacgatg aattattctt gtctttagca aactcagtct gagcaaggag
      541 ataattgtat gcgggatttt ccggatattt ttttccaaaa gctgctacca gttttttacc
      601 aacttttttc tccgaaagat tccgacaggc atcgacaaaa tcgtttgtca gttcttcctt
      661 attaaaccta tcatcaaaag aagcgagtcc aaacaacgaa gggtgcactt cctccacatc
      721 gactttaaaa tccaacaact ctgataactg cgcaaacaag ttgtcgatct ttctatcacc
      781 ttcaatccgg taaattaaac tcgtcgttat cccaatcaaa gaatgttcac caaaattcgt
      841 tacggcaatg gtttcaaaat tcagcaataa atttttcaaa tcattgaaag aataagcaaa
      901 atatttttca aaattgttgt tgtcatcatt atcgttgtca tcatcgttat ctgaatcaat
      961 ttcgctatca agctcagata catccacata atgatagttg ccgttgccaa ccgttttatt
     1021 gagtgtttca agaatttcgt atacgcgatc cggagtatcg aatggaccct gaacaaaaac
     1081 cggaactcca ttatcgccaa cttcaatatc aatcaattca atattgtcgt tatcttcttc
     1141 caaaataaac tgtgttgttt tatagaaatc agggtgcgac tttaagccaa tttccgttgc
     1201 aaagttttcg ccggcaaaaa caatattgtg agcaagttca taactaataa cagacatttc
     1261 ctgtttcgaa aacaagtcat ctttcaaatc ttcataatca tcggccgaaa cattaaacca
     1321 gaaaaaagta tctttcacgc ccaggcattt caaatcaacc aagtaattcc cgatggtgaa
     1381 atgaccgttt acatgctttc ggacaataaa aaccgttgcc agttgatttt ggttccattc
     1441 atcattcacc aaacattcat gaatggggag tgtccgtgca cgttggcgaa tgtatttttc
     1501 gggcgacaaa aattgagtca tttaaatagt attgaaaaat aatgctgcaa aaatatcaat
     1561 taaatgtcat gattcaaaag cacattatct attccaaaaa aaatatggtt atctcacttt
     1621 gaataatgat aagacttttc tatgtcctat aaataaataa ccccgaaaat cggtattcaa
     1681 agcccgtttg cagattgatc ctgcccatga cagaaaaaat acatatttta gcagcattaa
     1741 aatacctgaa tatgaaaatc aaggcattgt ttttatcgtt tgttctgttc atcagcatat
     1801 cgttacgcac actggccgac gaaggcatgt ggctgccact tttgctgcaa tcactcaatg
     1861 aaaaagagat gcagtcgatg ggctttagac tcactgccga agacatttat tccatcaatc
     1921 aaggcagcat gaaagacgcc attgtgttgt tcggacgcgg ctgcacagca gaattgatca
     1981 gcgatcaggg attgattctg accaatcatc attgcggcta cggccagatt cagagccaca
     2041 gtacagtgga acatgattac ctgacggatg gattctgggc catgagcaag gaacaagagc
     2101 tcagttgtcc gggtcttacc gcaacaatac tggtcagaat ggaagatgtt acatcgaaag
     2161 tacgggcaaa actgaccgac gacatgactg aagcacaacg tgaagacgcc ataaaagacg
     2221 tcagcgataa aatagcccgg gatgcggttt ccggcacaca ttaccaggca caggtgaaat
     2281 cattttatta tggcaatgaa ttttatctca tggtttatga agtcttcagc gatgtacggc
     2341 tggtaggtgc gccaccttca agcatcggca aattcggcgg tgacaccgac aactggatgt
     2401 ggcctcgtca taccggagac ttttcaatgt tcagaatata tgcaaacaaa gacaacaagc
     2461 cggcagcgta ctcaaaaagc aacataccat acaaacccaa ttacgtcttg cccatatcgc
     2521 tcaaaggagt aaaagaagat gatttcactt tcgtattcgg atatcccgga acaaccgagc
     2581 atttcaccat ttcagacggc gtaaaaatta ttactgaaga agaaaatcct gtagccatta
     2641 gcatacgccg aacacgaatg gacgtgatgg aaaaatacat gaaaaccgat cccgccattc
     2701 ggatcatgta tgcaagcaaa catgcaggtg tagggaatta ctggaaaaaa atgatcggcg
     2761 aaagcaaggg catcaggcag tggaacggca tttcgagaaa acaggcacag gaaaacgatt
     2821 tcatggaaac gttgcggcat gataaagaga tgtttacaaa atacggaaca ttgcttgaaa
     2881 acatgaaagc tgcttacgaa aacctgcgcc catactcact tgcatataca tatttctttg
     2941 agggagcata cagtattgaa gcattaaaat acgcatatag ctttacagca tatattaacg
     3001 aatgcatcaa aaatccaaaa tcagacctga tccctggagc aaaagacaaa ttacttaaat
     3061 cagtggacag ctattataaa ggatatcatg ctgatatcga caaagaaata gccattttac
     3121 tgatgccgat tctgaaaaat atgggacccc gatttctgcc cgaagcctac acaaatatgg
     3181 aaagcaaatg gaaaggtgat tggaaagccg ctatggatga tgctttcaag cgcagtttct
     3241 ttgtctctaa agaaaagaca ctgcaattcc tcaacatgaa tccccaaaaa gtgaaaaaag
     3301 ccatacaaaa agaccctgtt tatcaactga ccagtggatt tttcaatagt tactttgata
     3361 aaatagaccc cggccaaagc ttatacagag cgcaaataga cagcatgtac cgcatttatg
     3421 tcggcatcaa aaaagacatg aaaacatcgg aacgtatgta tcctgatgcc aacagcacct
     3481 tacgcattgc ctacggaaaa gtaaaagggt ttcaggcagc cgatggcgtg aactataagt
     3541 attatactac tgtggatggc attcttgaaa aagaaagccc tgaaatcgaa gattattatg
     3601 taagtccacg gctgaaagaa ctcgtacttg ccaaagattt tggaatttat gccgatgccg
     3661 acggaacatt gcatacggga tttattgcca ccaaccacac caccggaggc aacagcggaa
     3721 gcccggtact aaacgccaat ggcgaactca tcggcatcaa cttcgaccgc gtatgggaag
     3781 gcactatgag cgacctgatg tttgatcccg gtaaatgccg caacgtgtcg ctcgacatcc
     3841 gctattgcct gtgggtgatt gacaaatacg caggcgcagg atatctgatt gaggaaatga
     3901 agattgtgaa atagaaatat atggttgggc gtagtcttta ataaaaattg gttgggttta
     3961 gtctccagat taaacccagc agtaaaatct attaagacta agcccaacct cagagtcttc
     4021 actgtcgtct atccttcgac tcagctcagg atgacagaag catgcgttat aatgaaagga
     4081 gcaggaatca ctcaaacatc cagactgatt tcatttgaat tcttaaatat tttgacatgc
     4141 agaccaaccc aaacatattc attgattgca accgtgcgaa agccaatctg aaagctgttt
     4201 tcgaagacca acgattttca gatgttacat tcagaccaca tttcaaaact catcagagca
     4261 tcggtgccgg catgtggttc agcaattttg gcattgagaa aatcactgtt tcttcgttgc
     4321 caatggctga atattttgct gcaggcgaat gggatgacat tctgattgcc atgcctctaa
     4381 atccaaatca cattgctcaa tacaacacac tggcatcaaa aataaaactg catctgctgg
     4441 ttgataacaa tgatactctt tctattgctt tatcggaaat aaaaaatccg gtttcatttt
     4501 atctgaaagc cgatgccggt tatggccgtg caggctggcc tgccgaaaac accaaagaat
     4561 ttttaaaagc attaaagctg atttcaaaaa acggcacaca ttcgttctcc ggcattctga
     4621 gccactttgg gaatacatac agcgccagat cggttgaaga aatagttgac atcaatctct
     4681 catccattac tagactggtt gtattgaaag agtatattga aacacaaacc gggaaatcct
     4741 gcccgctcag catcggcgat acgccttcgt tgccccaata tacgcctgaa atgctgaaaa
     4801 atatttccga gttgcggccc ggaaattttg tatattatga tatgatgcag gtagcgcttg
     4861 gtacatgcca aatcaatcag gttgctgcag cgcttaaatg tgatattctg agcgtttatc
     4921 acgaccgcaa cgaagtgctg gttcatgccg gcgccgtgca tctttcgaaa gaagtctgcc
     4981 cgatgcccga cggcaaaccc ggatttggcg cgcttgtccg cttaaacgaa ggcaacatct
     5041 gcgaagtgat tgatgactgt tttgtcgatc gactttcgca ggaacatggc attctgcgcg
     5101 taacgccagc gtttttcaat gaatttggca ctggtgattc tattggcatt ttgccggtac
     5161 atagttgttt gatggtggcg gcagatagca gacaatgaca atttcaggat atttcgtagc
     5221 cgacgtctcc ttgtcagtca gataaaattt catgtcagca agctttgctg ttcaacccct
     5281 tcttgctgac gataaaaatg ttgggaggct gatgataatt gggttgtaaa taagtgtaag
     5341 agcaattcat gcatcataca acatgtgtta tttatttgtt attttttacg ggggggcgga
     5401 tttgaatatt tattaagtat atttgcctaa tatgttgaat tttatcatta tgttttatct
     5461 gttcatcaga agaacgttct tttttgctga taaaaaaatc aatatacgct tgaagtgcgt
     5521 tgcaatgccg ttggagacat ctattggcag cattaaagca aaaaaaaccg accatgggtt
     5581 tgcacccccg atggccggcc ttggttcaca tccaatcatt aaactgaatg ccgtgcaaag
     5641 atacgaaaca tagatgtaaa cccagtgaaa ttcaatgtta aaagtgaatt ttggtggcgc
     5701 gtggtaactg ccaagcggtt ggttgtactg cgaaagtggg aaaaaaaatg aatgtattgt
     5761 ataaataaat ctgttcacga tgaaaaaaaa tctgattttt gcatgtatga tcatgcttgt
     5821 ctcatgtttc tcctgtcaaa aagacattga gtcaatcaaa ccaagttcga ttcaaccaac
     5881 attaatgatt aagtcaatcg aagattataa accagaacca aataaattca tgcctttgat
     5941 tgaaaaggtt aagcaatatt ctggcgaaac aaataacggg aatattaaaa cacaagaaaa
     6001 cgactcaatt ctaattcagg atgctttctg gcaacttgaa acagcaataa actattacta
     6061 tggaattaca ggagctccca aagacacaat tattgaagat tctttgattt taaatcttcc
     6121 gttttcaatt gaggggagtg aaggttatgt aatgattcct gacgtagctt cattatactc
     6181 tgaaatgaaa gaagaaattt taacttattt ccctcaacac attcttcttt atggggattt
     6241 aactgctaat gaagttgaag gaaatattct gaaaatggta ttcaacttta ccatgtccaa
     6301 tccaccttca gatttaatat ccggaaatgg gaacaaggac ttgatctttc ttgatcaaac
     6361 atataattgt tcaacatgcc gtgttccggt tgccatcgat ccatacacag ctattgtaac
     6421 gccatttaat tcaaatgaat cctatcctgc aatttttgca caatctggga cacctggaac
     6481 acttacattt acgggatctg cgagagaaaa gatacaaaac aaactcaacg gatcggcaaa
     6541 tgcaattcct gcgactcccg gatttaaaat tgtattatca aactattcaa acaataactt
     6601 ttttacttat aatggatcct ataacttgta catcaatcca ggttcattat ttggagtaca
     6661 aagcaatcct tgcgaaatac taaattcggt tagtttaaac atatacttgc aaaaagcaat
     6721 tcaaatgatg cagtttgctt ctccagccgc tccaagaaaa ataatttgcg caaaatatag
     6781 ttgggtaggg ccaactgacg atgggatacc tgatccatac cctgcatggt cctggatttt
     6841 catttgtcca acagcggaag taacatacgt cccaattaac caaggaacaa attaatcatg
     6901 aaaagcttat tgtttgtatt attcctgtta gcctgtactt tgctgaaggg acaggggttt
     6961 gagaaaattc gttttggttc accagacact gcatatgtcg gagtatttac agatgagttg
     7021 actgaaggca aatacactgt gttgtgtggc caatatgttg atcacattcc tgtgggaggt
     7081 gaaattcttg aatacgatgt ttcgggctca cttgggaaat atgtaaaact gcccgaatat
     7141 acaggattga tacttgaacc tgaacgaata atcagatccg gcattaacca gttgattctt
     7201 gcagggaatg catggaataa tacaaacgat gagcaggatt tgtttttaat gacattggac
     7261 aactcatatc aaattcaatc tattgtcatt gtgaacaatc caacacttga tgatcgcttt
     7321 ctgaactggc attttttcaa tgacagcata gtggatattg catgtaaaga tttggacagt
     7381 gcttattaca attcatatgt tttaagggtt agactgaacg gacaaattat caaacagact
     7441 acacctggct atcctgtcaa cccggttgat ttctattaca aaaaatcaga cagcacatat
     7501 tatatcatta actccgatgt cattttcgga ttttcggcag attcgctgaa ccctttagga
     7561 attctttttg accaatttgt ttataatcag agccacaatt acccggttct tcagacagga
     7621 aacgcatttg gaataaacga cagtgtgttt ctgactatgg gacattacat ggatttttcc
     7681 ggcacaattc caataagaga aatgggcata agcttgtgga ataattcgtt tgacccggtc
     7741 atcatttttc ataatggtga agatggatat tataatcaaa gtcctagtaa cagcttctcg
     7801 tttattgatc ctaaacatgc attcctttca ggatattcat tgctactacc tgatgggata
     7861 aaacaacaaa ctactttgga cgaatatagg attctagtct ttaatataaa cacagaaacc
     7921 ggagctgaaa attggagaag attgttttac agcaattatt gcgcctttcc cgatttcact
     7981 tcttctacca gcgatggagg ctgtctgatt acagggtcac gtacggatct aaatggtatc
     8041 cgcacagaca tgtttctttt gaaacttgac agccttggga attgcacccc tgatgttatc
     8101 agtgaaaatc aaaagcctgc ctacgaaata ttccctaatc ctgtgagtac atacatgcga
     8161 gtttcatcgc aaggttcaat aatttctttt tccatatcag acttgtcagg cagatcactt
     8221 gtgaacgaaa aatgttacgg aaacaatgac atccttattg aaaccgaaat gctagatccc
     8281 ggcatatatc ttcttaaagc aagttttaat gatggaagta cagctgtgaa aaagttttta
     8341 cggttgtaat tctactcttc cgctcagacg aaagtttgtc cgctcatcaa ttcacgacag
     8401 tgatagatgc cggcagaatt ctatgtcagc aatgctgggt gtcatcaatt tcttgatgga
     8461 gtttattctg ctgtctcctt ccctgcccgc tgcgcagcag gcggggatac attttttctt
     8521 tgtttcactt cgaaaaaaca ctgctaatga catatctaca taaatattta aaaatcaaca
     8581 gaagcaaggt tgagttcccg ggcaagcctg aatcagcttc gaaaaagcag agcctgcgcg
     8641 acccgggatc tcattctata cggcagattt cttctgttga tttcgctgtc tccttcgata
     8701 cttcgcaagc tatttcacaa gaagcgctac aggagcgaaa ctcaggatga cagcaactta
     8761 aagtgtcatt ttttatttag gttgcaaaac gcattttgct ggtgcttgtc gcgtaatgtc
     8821 agcatgactg cacgcgggtc tcaggatgac agcagggcat agcatgacag cggcgaggat
     8881 agaatgtcgg cgaaactgct gctcagccaa ctcttccatg ttcttcttcg gcgaactatg
     8941 gaaggttggg gagatgtgtt tttgaattga aaaaatagta catttacgca tcaaaatttt
     9001 tgttctatgt caattccaac tacccgaaca aaagaagatg ctgtgatcag catcaacgtg
     9061 gaaatctgca ccggatgcgg gctgtgtgta aaggcctgtt gcagtgatat gctgaaaatc
     9121 gaaaacggaa aagccgctga attgcagggt aaatcggttt tcaactgcat tgcgtgcggc
     9181 cattgcatgg ctatttgtcc tgagggagcc attaaaatca gcggcagaac catgtcgccg
     9241 ggcgacgtgt tcgacattcc aaaagacgca tcggatgcga catatgagca gctcatgacc
     9301 ttgttcgaac ggcgtcgcag tgtacgaaaa ttcaaccaga aaacgattga gccggaagtg
     9361 attgaaaaaa ttctggctgc tgcccgcact gctcccatgg ggcttccacc cagcgatgtg
     9421 catgtgctaa ttctgaacgg acgcgaaaaa gtaacagctt ttaccaggga ttttgcacag
     9481 tatctgaaaa gtctgaagta tctggtgtcg ggctggtttc tggccataat gcggcctttc
     9541 tggggcaaaa ccaatgatga aatgttccgc aattttattc gcccgttgtt tgaaaattat
     9601 atcggtgaga tggagaatgg caacaattat atcacttacg atgcaccact ggccatgtat
     9661 ttttatggct cgccctactc cgatccggcc gatcccattg ttgcagccac ctatgccatg
     9721 catgccggcg aatcgctggg actcggaacc tgtatgctgg gcgccattca tccgtttatt
     9781 caacgcggta aaaaagcagc acaattccgc aaagcgcacg gcataaaata taaaagccag
     9841 gaaggacttt ttgtgatatt cggctatcac gatttgaaat ataaaaaagg catcaaacgt
     9901 acttttgcag atgaaacttt tgcatagaaa atactttata gttttgttgc tgctgtttcc
     9961 ggtttgcgct tcgggacagc attctgacac gatcagaata aacaaagaca ttcagctgat
    10021 tcaactgaaa gattcttttt ttctgcatgt caccaccgat tatgtggaag gctttggaat
    10081 ggtttcttca aatggtttga tcgttatcag aaacggtaat tcgctgatga ttgacacgcc
    10141 gatggacaaa ataaaaacat ctgcaatcct tgattatctg cgcgattcga tgtacacaac
    10201 tgtcactgtt tttattcccg gacactggca caacgactgc attggcgggc ttgcagaact
    10261 tcatcgcaga aatgtttttt caattgcaaa tgaaatgacg cgcgcagaat gcatcaaaag
    10321 aaatcttgaa gtgcctaagg cgtcgttttc gaaatacctg acatggtcat tctgcggaat
    10381 accgctcgaa tgcttttatc cgggtgcagg acatagtctt gacaatattg ttgtgtattt
    10441 tccggaacag aaaattcttt ttggcggatg tctgattaaa tcggccgaat caacttccat
    10501 tggaaatatt gcagatggcg atgtagaagc atggcccaag acattgaaaa aagtgcagaa
    10561 aaaatatccg gatgctgaaa taataattcc aggacatggc tctgtgggct cgttgaaact
    10621 gattgatcat actatcttca taatcaatca ctatttgaat agtaaaaaat aaattccatt
    10681 attttaatgt taccgataac tgatttaatg ttgacatttt aatgtttgat attaatttta
    10741 tgcaattaat ctcgaatatc atgaaacatt atatattcat tttcattgcc tgctcatatt
    10801 tatctggtgt tgcccaacag ggaaaaccct gtaaagacat tagtttcaaa acaccgcaaa
    10861 aagccacctt ggaaactgac aacggatacc gggccgactc atgtgaatat cactgtctgt
    10921 gcgagccaca gagtattgat ggtgaaaagc actattattt ttatgatcat ttgggaagag
    10981 acacttccat ctggaatttc tatcaggaca cgattattaa cccgtggtat cttgaatcaa
    11041 aaaacacaaa agaatacact tcgtttaatg caattcaaaa tgaaaaaggt tattggtatg
    11101 atacgcttgg catgtggcaa tacacttttc tgagacaaaa cactttcaat acatcgcagg
    11161 aaatgattca tcagaaaaac tatcgacttt atgagtccac tttgccacat agcatcagtg
    11221 atttcaccta tggccccaat gggttatcgc aatctctata ttttgttgct tatccaactt
    11281 atcccgcaac attgcggtcg aaacaaacat acttttacaa cgtttcaggc aatctgagtt
    11341 atattgaata ttactcctat aatacgcaga catccacatg gtctcctaca gtttcatatt
    11401 tctacacgta tgattcactg gaaaatctga ttcaatatag tcgttttaat tatgccagcg
    11461 gagcgccttt atgtgattac aaggaacaat acgaatatga tggcaatggc aatatgacat
    11521 taaagacaat ttcaacaatt gagtatggca atccatctct tcaaatcaca agaacaattg
    11581 agacattttt caacacgttt aataaacctg attctgcaat aacatctgaa tactatatgg
    11641 gtacttcatt cgacgggaag ctggaaaaat attattacga cagtctttgc aatcagatac
    11701 gatgggaata ttatttgcat tggcaaggta attcttcgtg gcaattattt ggatgggaag
    11761 attgttatta ctcgcaacat attccaacac acggcatttc agaaccagct actaatcttg
    11821 gtttcacagt atttcccaat cccgcatccg atattatacg cgtcagttca gaggaagcag
    11881 aattttcaga aatcaccatt atggatttgc gcggcgttga attgatgaga ctaaatgccg
    11941 aacagagcaa tcaggtttct gcaaacattt cagcactgtc caaaggcatt tatttcatca
    12001 gatgcacagc tgaagacgga aagagtggaa tcaaaaccat tgttgttcaa cgctgagcat
    12061 tatcccagag cgacatcgag aaacatcatg agggcgaaac cggccatggt acccatggtt
    12121 gccagatcat tatgttttcc gcgctgcgac tcgggaatga gttcctccac cactacataa
    12181 atcatggcgc cggcagcaaa agccagtgcg tatggcagca tggtggtcat gtgtaacaca
    12241 gccaatgcgc ccagaatacc gcctgcaagc tctaccatcc ccgaaaactg tccggccata
    12301 aaagatttca tccgggtccg gccatcacga aacataggaa tcgatacggc agcaccttca
    12361 gggaaattct gcaagcccat accaatggta agagcaatag ctcctgccag tgtggtacct
    12421 tcaataccag aagcaagaga gccaaaaaca acgccaacag ccagaccttc tggaatatta
    12481 tgcaatgtga tggccaaaac caacaaagta tttctgttcc acttcaccgg gatcccctca
    12541 gcttctgact tattcattcc actatgcaaa tgaggcaaca aacggtcggc aagacgaaga
    12601 aaaacagccc cggccagaaa cccgataaca gcaggaatcc agccaatgcc accacgggcc
    12661 tcttccattt caatggccgg actcaaaaga ctccagaagc tggcagcaat catcacgccg
    12721 gctgccagtc ccagcatgac atcaagaagt tttctattta tttcacgaaa taaaaaaacc
    12781 gtagccgctc ccaatgcagt cataccccag gtaaaggttg tagcaatggc tgcctgacca
    12841 acatgaccaa tagattcaaa ccaatccagg aaactcatta aataactgtt tcaatgattt
    12901 tacgatcaac tttatgatag aatttcgatt caatttcatc gcgttgttcc aaaaatgcct
    12961 gaaattcatc acttactgca acacgttgct gaatggtctc ttcaaaaata tcggtcaaac
    13021 ccatcacaag ttcacacaac tcacgaacag cgttaagatc accgcgctga tagctgatat
    13081 aatcaaccaa atccttttcg tgaacatagt tcataaaaag aggacttgac ttacgtccaa
    13141 ccataaaact cagtccggca acatcagaag cgctcagatc aagcacatcg tccatgataa
    13201 aagcaacctg tgtcggacga agattgaaag tgtcgttcat gtgggtgaat gcctccttct
    13261 ttgatttgaa attaacatac acggcattgt aatgttcgcg ttttgcaaga tcaaatgccg
    13321 actcagtaat ctgattggta accaacccga caaaaggaat ttttttgtag cgcaaccaat
    13381 aagaaaaacg aagaagatta attccaagac tgtcggtttc gctgtaggta gaaggaaatt
    13441 ccgagctctt tttaccgctg ttgaaaacac catcccaatc gaacagaaat ccgtgaatgt
    13501 tttcaagttt ttttgcgatt tttggagcag gcgtcataaa cacacctcca tgcttttcaa
    13561 acaattcctg tattaattcc ggtttcatat agatgaattt aatgcactaa gatacaaaaa
    13621 aaattctatt tcaggcctta ctgattatca actgccaggt actacgcgat ttttgttcaa
    13681 gcaacacttt acgtcctgca ataaattcat cagccgaatt gcttgaataa tgcctgattg
    13741 ttacaaggtc aaccgaatca ttataacgca ccagaaaacg tttctgaaga tcttcaatca
    13801 ataccgacaa tttagcaggt tcgaaatcaa aacaaactga aaaactcaca gcactgtttt
    13861 gcatagtgcg aaccttaata ttattgcgcg aaaacattgt aaaaatctca ctcatatttt
    13921 tctcattaat gaatgaaaaa tcacgaggaa aaattgacaa caacacctga ttaaatttaa
    13981 aaatatatga cgcaacagat ttatcgtcac tcccgtttcg gtcaatggta gtgcctgttc
    14041 ctgcaggatc gacaaatgac ttcacactca ggggaatgtt tttattttcg agcggtttga
    14101 ttgttttcgg atgaataacg gttgccccat aatacgccaa ctccacggct tcgcgatagg
    14161 aaatatgcgg caaggacact gctgatgaaa taagcttggg atcggcattc ataatgcccg
    14221 gaacatcttt ccatataata acttcggcag catttaaccc gtgcgcaaag atagatgcag
    14281 aaaaatcgga cccctctcgt ccaagcgtaa tcgggcaacc ctcttcggaa cggcctatga
    14341 atccctgcgt aagcacaatt ccggttttgg caaaccatga ttgtccggct ccggaaatcc
    14401 gcgctccggt ttcttcccaa ttgacatatg cgtcgcggta agtgttatcg gcaatgatat
    14461 aatcacctgc agcgatatgt gttacatgaa acccggcatc aagcagaaat gcactcagaa
    14521 tggttgttgc aatgcgctca ccaaaggaaa caatccgatc ataacttttg tcgaacgaag
    14581 attccggcgc aatatcaagt tcattctcca gtgcgctgaa taaatctgaa acagcattga
    14641 aaaccgcatg atcacactta aacagctcat tcaaaataga atcatgaaac tgttttgatt
    14701 ccgaaaccct tttcagcgca tcggattttc ctcctgaaac ataggcctga tgcactcttt
    14761 cgagagcgtt ggtggtttta cccatggcag aaagaatgac caccagattc tgattgtcat
    14821 attttttcag aatagaggcc acattacgaa cagcggcagc atcctttacc gaagccccgc
    14881 caaatttaaa aacacggaca gctgattgca tttgaaaaat ttttcattag cacaaaagta
    14941 atcaataatt tgcctgataa atttgaaaaa tgaacccgct aagtgaatta ttatttatct
    15001 tcgcagtcaa acaacaatat catgcttgtt atcgggattg ccggcggaag cggttcagga
    15061 aaaacaactg ttgtaaaagc agttatgcag caatttacca aaggagaggt ttgtcttctt
    15121 tcacaggatt cgtattatcg cgacaacggc cacctgagcg ccgaagaacg tgcccgcatc
    15181 aatttcgacc acccgtcatc catcgaattc agtctgctgt gtaaacacct cgatgctctc
    15241 aaagccggcg aagacattca gatgccgatc tattcctatg taacctgcgc ccgctcaaag
    15301 gaaaccattc cggtcagtcc tgcccgagta atgatagtgg aaggcattct ggtgcttgcc
    15361 aatccgcggc tgcgcgatcg tcttgatatc aaagtttttg tcgatgccga tggagatgac
    15421 cgcctgatgc gcattatccg tcgcgatatc gaagagcgcg gacgttcctt ccttcaggtg
    15481 ctggaacact acgaacggtt tgtcaaacct atgcaccaga cctttattga accaaccaaa
    15541 cgatatgccg acatcattat cccacagggc ggcgccaata aaatagcgat tgacatatta
    15601 accgcaaaaa tcgggtccaa actcgccgaa tacaatggca gtctatcgtc ttcccaatga
    15661 aattgttttt cctgatccgc acgaagcaga acccgatgga ttgctggctg taggtggcga
    15721 ttattcggtt gagcgattgt tgctggcata caatcatggc attttcccat ggtttcaata
    15781 caaaggtgat atttactggt atgccaccaa tccacgtatg attgtatttc ccgacaaata
    15841 cagacccaat catggcttac gtcgtgtgtt gaaaaaatgt ccctatcggg ttaccatcaa
    15901 cacttgtttc gaagaagtat taaatcaatg cgccaaaata aaacgtaagg aaaaaggcac
    15961 gtggattaat aaaaaataca aaaaagcatt tattgaatta catgaaaaag gatacgccat
    16021 atctattgaa acatggcttg gcgacgagct tgtgggtggc ttgtatggga ttgtggccgg
    16081 ttcgggattt accggtgaaa gcatgttttc ggcacatccg aacgcatcga aagtggcttt
    16141 tcaccagctg gtcatgcttg cccgccattt gaaatggaaa tttatcgatg cacaacagga
    16201 tacgccgcac atgcgcaccc tgggcggtga actcatttct tttgacgatt tttatttatt
    16261 actgaccgaa taattcataa cgaatatcca tttcaatctg catttttttt catttcagat
    16321 tgtcgtatct ttgcatatta aaatctgatt ttcctgatga aatacactat tccgtattta
    16381 ttttcggaat ctgtcaagaa gaattccgag tgtccggcac tcacatttgt aggagataaa
    16441 cctttaacgt attctgacac ggccaaacac atctgcaata ccattggatt tttacaaaac
    16501 agggacattt cgaaaggaga caaggtggcg ctttggggca ccaattcgcc gcaatgggcc
    16561 atcgcatatc tggccatcac aagcatgggt gctgtcgtag tgcccattct tccggaaact
    16621 ttaccagccg atgttgttaa tattctcaac cactccgaat cgaagctgat ttttctcacg
    16681 cagcccttgg ccgtaaagct atccgaagaa aaagtatctg atagtgtcat acaaatcaat
    16741 ctggaagagc attttgacga agccccggaa acccagacca cctgcgactt tgatgttttc
    16801 gatgtacaag aaaatgatct ggctgccatc atttacacat cgggaaccac cggacgagcc
    16861 aaaggtgtga tgctgaccca ccggaacata tcgcatgtcg gctggcacag caacgacatt
    16921 caatccatca acaaccagga tcggtttcta tcggtactgc cactggctca tacttacgaa
    16981 aatttgatcg gttttttgct tgccatgttt tttggtgcct gcgtatatta cctccgcaaa
    17041 gcacccacag catcagtttt gatgcctgct atgaccgaag taaaaccaac tttgatgcta
    17101 actgttccgt tgattataga aaaagtatat cgtcagaaaa tcatcagaac cttctcgaaa
    17161 aaccgcttaa tccgctttat atatagcatt cctgttttca gaaaactgtt gaacagaata
    17221 gccggtaaaa aactatatgc atcgtttggc gggcacttga aatttttcgg aatcggtggt
    17281 gccaagctga atgcatccgt agaaaaattt ctgcgtgaag caaaattccc atatgccatt
    17341 ggttatggac tcaccgaatc tgcacctctg attgcaggag ccaatgcaca atacacccgc
    17401 tatcagtcaa ccggtccggc atatacaggc atttccataa aaatcaacaa tccaaacccc
    17461 gaaaccggcg aaggcgaaat atgggcaaaa ggtcccaatg tcatgcaagg atattacaaa
    17521 gaacccgatc tcacagcgga agtactgaca actgatggct ggctcaaaac cggcgatctt
    17581 ggcaaactca gcaaagacgg atatctgtac attttaggcc gatcgaaaaa cgttattatt
    17641 ggtgccagcg ggaaaaacat ttaccccgaa gaaatcgaag cgctgatcaa caattatccg
    17701 ttggtagcag aatcgttggt tttagagcgc aaaggacgtc tggtagcgct tgtacagtta
    17761 aatatggaag accttgaaga acaaatcgcc gagctgaaat caaacattga agcccgcaaa
    17821 gaagaaattc tggaagacat caggaaatac gtaaatgaac gcgtaagcca gctttcacaa
    17881 cttcagtcgg ttatgtttca gccaacacca ttcgaaaaaa ccgcaacgct gaagatcaag
    17941 cggtatctct atgcgtgatt ttagagataa gatttgagat ttgagatttt tgaaggagag
    18001 cctgaatttt gaatgaagct tcttcttcct gacttcgcca ctgggacacc cattttttct
    18061 gaagtttttg aggaaattca tccaataaaa acaaaggaag agtgtaatac caaaaggaaa
    18121 tataatttag tccaaatgtg atttgtgtta ttttattttg tatctttgga ctattaaata
    18181 attcacatgg cagcaccaaa gatcataatt gttaccgaaa atttgcagga attgaagcag
    18241 attttgaagt ccacatcagc gtttctggct cccagagtgc gtatgctgat agaattaaaa
    18301 agacatgaaa acagtggtat ttctaaaaga gatctggctg agttgattgg cgtgaatcac
    18361 aatagcgttc agacatggag aactgcttat gagaaaggtg gcatcaactt acttttgtcg
    18421 cataaaaaaa caggttataa gccatcggtt atcaccaaag aagagcatcg aattattgag
    18481 aaaaaactga atgatccgac gaacaatatt cgtggataca aagagctgca gcagtggata
    18541 gaagacgaat tggaaaagaa aattgtttac agcacattga tgaagtattg cgttcgcaaa
    18601 tttggatcgt ccataaaagt tggacgcaaa agccacatta ataaagatgt tgaagcagta
    18661 gaaggtttta aaaaaaattc tcggaagtct gcatccaggc aatcgaaagc aaaaaagtac
    18721 gatttgaaaa agtaaatcta ttttttcagg atgaaagccg ctttgggctg tttacaagaa
    18781 atggaaaagc actaacagcc aaagctgtaa aaccaataat tccgttccat caggtattca
    18841 aatcactcta tctctatggt gctttttcac caataaatgg tgatggtttt ttgctcgaac
    18901 ttccggcttg caatgccgat ttgtttcaat tatttctcaa cgaattttca ctccaaaatc
    18961 cagacgaatt gaaattaatt gtgctggaca acggtgcatt tcataaagct caaaaactgg
    19021 cgatcccaga aaacatagtc ctcatctttc ttcccccgta ctcgccagaa ttaaatccag
    19081 ctgagaaaat ctgggccaaa ttcaaaagag atttcaccaa ccgtctgttc catacacttg
    19141 attcccttga aaaatacttg tgcgaacttt cttcttcgct gcaaagaagc gaggtgattt
    19201 caatcacatc ttttagctat gtgttttctt gtccgctttg aactaccgta taattcactt
    19261 tgatattaga tttttattga tgggtacgtt catgcttaaa ggcacatcca gagccatgtt
    19321 caatgataca ttgaccggaa tggaaatggg aatggaaatg cttgttttaa ccggaatggt
    19381 gtctttcaca tctgcttcga ccatgaagct gctgttgccg atgctttcaa tgatatcggc
    19441 tgctgtattg agtttgtttt gtgcatgccc gattttatca aaagcaaaaa aagcaatggc
    19501 tgaaatcagc agaatgctta ttgaaatgat aatggctgtg cgggttttga aattcattat
    19561 tcgggttcga tatttctggt ttcagcttcg aaaatttctt tttttaacga aagttgataa
    19621 ccaaaaaaca gcgaattgag caaaaagcca atagtggtaa gataataccc tgttcccatg
    19681 gaaatgatga taaccattga tccttcgctt ggaatatctt ttcgtatatg aaaataaaaa
    19741 tacaccagag ccgctaaacc cagaacgctg acaataagcg atgctattgt gtttcccctg
    19801 aatttcagaa aggccataat caagccggca actgcgagaa tcattgatga aataataaaa
    19861 atatcaggat taatattata gggctcttcg tcgctgtcag cttctatttg tgtgcctgtg
    19921 gccagatgga tgccgttcat tttagcaatt tcgtaattgc cacatttgac agtaacaaat
    19981 ggcagcagaa agcaaataat tatcagcgca aaagacatct ttggaaagtg tggaaatagt
    20041 ttcatgtctg tacattttct ggttcattat acaaatataa cgaattcggt aatagatttg
    20101 tttgagccga taaaaaaccc tacagagtct caatgctgca aggtttttta tatcgcacct
    20161 tttaactatg tgatttttgt ttgttatttc agtttctcgg ccagccggcg catttcctga
    20221 ctgggagaaa cattattgta gcagatattg tacacagcat tcagaatcgg catatctact
    20281 ttgtattctt cattgatttc ggacatgcat ttcgaagcat agtatccttc tgctatttgt
    20341 ttcatttcaa tgcgggagta atttacactg tatccttttc ctatcagatt gccgaacatg
    20401 cgattgcggc tgaattggct gtaggatgta acaataatgt cgccgcaata ggccgtcatg
    20461 ttcacatcgc gtttgcaatg gttgatggca tcgagaaaac cacgcatttc catgatggca
    20521 ttggtggtaa gcacactcag aaaattgtcg ccgtaaccaa gtcctacact aatgccggca
    20581 gctactgcat atatgttttt taaaacagag gcatattcaa ttccaacaac atcggaagaa
    20641 acagaaattt taatatttct cgagcccaga agttctgtca atacagtgcc gatgtcttcg
    20701 tttaccgatg ctgcagtgag gtacgaaaga cgatccatga caacttcttc ggcgtgtgtt
    20761 ggaccggcaa tcattccgta attttctgca ggcacatcga aaacagtgtt gaaataacgg
    20821 ctgataatgg aattgaattc agggatgaca cctttgatgg ccgaaataat tattttgtcc
    20881 tgaaatattt ttttatccaa tgattccagt tgctgatgca gaaatgcagc cgggatgcag
    20941 aaaatgacaa tatcggaatt agaaattact tcatccaggt tgttgctggc aacaattttt
    21001 gtaggatcaa gttcaacctg actcagatat tcgggattat gtcttgagtt gcggatagca
    21061 tctatgactt cctgccggcg gatgaaccaa ttgactttct tttggtggtg ctgcagtact
    21121 ttgataatgg ctgtagccca gcttccacct ccaacaattc ctattttatg agcaattttt
    21181 atcatttttg gtcagaacac tacttctacc gaattttcaa tttggtcgcg aaggtaagta
    21241 atttttgtct tatcaccttt tttgaatgcc gaaagtgctt tcatataact cataatgtcg
    21301 gttacggtac tttcgccgat ctgaatgaca atgtcgcctg acagcattcc tgcctgatca
    21361 gctgtttttc cttttgtaac agcatcaata cgcattccct tgccttccca tgcatgatcc
    21421 ggaattacac ccaatgttac tccaggtctt tttcctcgat tgcctgaggc cgattctgtt
    21481 ttctgaaaat gaagcttttc ccgcttgttc agcttttcgg tgatttccaa ggcaatcaac
    21541 agaacatcag ccattccgtc acgattgatc ttttctgtat catccgatgg tttgtgatag
    21601 tcttcatgca tgccggtgat gaaaaacaat accggaatat ctttcaggta aaatgacatt
    21661 tgatcggatc cggctgtacc cgatgcactt tttctgatgt ttattgttcc ggcgtatgat
    21721 tgttctatca gactatccca ttctgcagca gtacctgttc caatgatgtt taatttcgga
    21781 ttttccttgt ccattctgcc caccatgtca aaattcagca tggccacaaa cgagctgtcg
    21841 ggcaggtgat tctgatttat aaatgccttg ctgccgagca gtcctttttc ttcggcactg
    21901 aatgcaatga aaagaaaatt gtgttgtggt gattcaattt tattgatttg ctgagccagt
    21961 tctattaaaa ctgcaactcc gctggcattg tcatcggctc cgttgtgaat ggcgctgtcg
    22021 ttgctcaatg agccggcaga gccgtatccc agatggtcgt aatgcgctcc gattactatc
    22081 gtagttggtt tattgttgtt tatccaggca gcaacattgt agccggtttt ttcatttttt
    22141 gaaaagatgc attcgctgcg aaggtttgca gtcttgtttt tgaggatggt ttttgaaaga
    22201 tcttttcccg ctccgataac gggtatgttg gctctgtttc tgtcggtatt gaatgcagca
    22261 gttaccggaa aattctcatt gttgctacta acaagaatga cagattcggg attgaatcgg
    22321 gtggctttgc caactgctgt ttccaacagg cctgtgtatg actgggattg actgttgctg
    22381 cacgaatcgg aatagtttat atcaatgatg ataacaacgt ttttgttgaa ggttggattt
    22441 aactctttgt atttttcgtc actgagtcca tcctctgcaa aaaatatttt tcctgtaaaa
    22501 gtgtaattta ccagcgggtt gatatagaaa aaatcctgat tgagccggaa cggtttttca
    22561 ttgatataaa cagttccgga tgtataggaa taaccttcgg aaaattgaaa aggtatgcaa
    22621 taatcttttt ctggaagtgg ttttagtccg gtattgctga attctaaagc aatatattgc
    22681 atagcttttg cttcgccctc ggtgccggct tcacggccca gcatgctgtc atgggccagc
    22741 gctccaacat gattgtacag gcgatcagcc atcgcatgtt tttcttcagc agaaatattt
    22801 tgaagaaatt gcccctgcag aaataatgac tgcaggagca ataatgaaag aataaaaaat
    22861 cgatgcattc tgatttattt tacaggccaa gatttgattt gccctgtttg aaaacgatgt
    22921 caaccggaat tttattttct tccagtttgt caagatcttt ctgaaggtcg gctgggataa
    22981 tgccgtcagt ttcaacccat ttttttgcgg ctgcgtagtc accttcacct tgaatgacta
    23041 aaattttgtt tacaagtgat tcgacaacgg atggcatttt ttcaaagtca accacataaa
    23101 aacctttgtc attacgtgaa atggcgccgt ttttcatgaa atagttgaag cacatcatgt
    23161 tggcttttcc atgcgcgctg gctgcgccaa aacgtacgga acggaagatg cctgcaacaa
    23221 aagaaacata gttttcttta atgtctcctt cggttatttc acccatttcg cgcagtttgg
    23281 ttacaagata caatccggtg atgtctgctt ttccctcttc gattgaagaa tacatgtctt
    23341 gcagcacttc tctgactcca ccttttccat tgatggtgtt tttgattccc agaccgtgag
    23401 ctacttcgtg gaacatgaca ttgtggaaaa aggcatcgaa ttttacatac tgacgctgag
    23461 aaggatccat caggatgttg gcaatcggaa gcaaaatttt gtcgaatttg gcttgcattg
    23521 catttttcag ctgcagttta cgtgtgcctt tcgacaactg aactttttca tcatttggaa
    23581 ggttgatggc aatggttttg ctgccggcat tgcagtcacc tgcataataa acagccgaat
    23641 atacgttcat gtcgctgtcg gcgccgggtg tttccttttt atatttttcg tcaacaggta
    23701 attgcttttg tagctcaggc agcagctttg cgaaacgttc cagctttttg ctccattcga
    23761 tgtctttaat cagaatgaat gattcaaaag cagctttata gccgaaaagt gcgtcttcgt
    23821 agttttcgat gggacccaca acaaagtcga tgttcgatgt cttcatgtcc atccaggcca
    23881 tgtcgcttgg aaaataatcg gaggtgatga gcgctttggc acgtagttcg agatattttt
    23941 tgaaccctgc gtcttcggcc agagctgcgg cttttagcaa taaatccgat gctttctgca
    24001 attgatcttt aaacatttcg tgataccata caactttgag cgttttgtct tcgttgcgct
    24061 gaataacagt gtataagctg gttttgtttt tgtcggccag tttttcaaac tcttcttttg
    24121 tcatgtcggc cggatagaat gtggctccca gcggcttttc gccaattccg tttacaaatg
    24181 gtttattgtc gcccagacga tcccagggtc catagttgat ttcggcaaat cgttttgtgt
    24241 ccggatcttt gatggtgtcg agaaatgcat ttttgtcacc cagcgtttgc atccaataaa
    24301 gtccatccat gatgtcggcc acttcaaaaa gaatggggat gatttgtttt tctttttcag
    24361 tcagatgact gatattggcg gtgagttcca ccatggcata ctgatcaact ttgtctttca
    24421 tggttacacc ggttgagtct ttgttttcat ctgttttttt cttgtctgat ccgcacgaag
    24481 tcaataccgc tgcggataac aatactgaaa gtattaatgc tgttcttttc atttgaaggt
    24541 aaatttaatg ctaaggtata aaaaaatgaa atactaaatt cttttcagca gtgagcagcg
    24601 atcggtgacc agtgagcttt gcttaaaaca agaagaagct tcattcaaaa ttcaggctcc
    24661 tgacttcgac actttgaaat ttccaatttt gtaagaaatt gatatataca tt
//
LOCUS       DAOPKE010000036        24501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2160_length_24501_cov_13.053219, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000036 DAOPKE010000000
VERSION     DAOPKE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_24501_cov_13.053219"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1401)
                     /locus_tag="PKY63_06370"
     CDS             complement(94..1401)
                     /locus_tag="PKY63_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00274.1"
                     /translation="MKKALLLAAVFVLTIGSGIGQTVFYSDFETWVDANTPTNWIGSA
                     TSITYTDVDQYTTNPYAGTYSMQINNDTTTHKRVTTQPVSLTAGTVYDISFYARGSGE
                     IRTGLYDGGSSYTYNSYVILDGTTWTKIEQSIICDTTTATAEFILSVRNTSATNDHLQ
                     VDSFYVTVGTLSNVSIYDIQYTTTDASSYLGQPVNTGGIVSAVKSGAYWIQSGSGPWS
                     GVYVYDSGNSPTVGDSVTFSAVVDEYFNLTELKSVSSYVKVSSGNPVQVTNINIPDGA
                     EEMYEGVLCKVTNAECTIMPDSYNEWVVGDGMDALKVGDLIYTSVPVLGTHYDITGVM
                     DYNYSERKIQPRSAADVTIYNAIDETEISSSVYPNPASDFVTVSAEAEGILKVVDMTG
                     RVVYSSIFSQQASIDVSEMEAGIYHVMITGNDGSSATIKLIVK"
     gene            1472..2566
                     /locus_tag="PKY63_06375"
     CDS             1472..2566
                     /locus_tag="PKY63_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPF00275.1"
                     /translation="MKCKELFKRIEDSVPLSWQESYDNSGLQIGNPENEVEGVLYSLD
                     VTPFAVEKAVKEGCNLIISHHPFFFHGLKSIDLANVTGGIIETCIREKIAIYSAHTNF
                     DKHPSGVSAALANAIGLEKPEILSPENGMLKKLVTYCPEAQAESVRNALFSAGAGHIG
                     NYDSCSFNASGLGSFRAGENTNPFVGGKGKVHFEPEIRIESVFPKDKQTAVLSALFAF
                     HPYEEVAFDIFSLDNQYSECGLGIVGNLSQPLSANDFISFLKTRLNIQTVRCNTNAVA
                     QISRVAVCGGSGASLINTAVRAGAQAYITADLKYHDFQAADGRIFLIDAGHFETEIFA
                     LSALKLLVSEILPNFVPQIISPDSNWVNTV"
     gene            complement(2574..3119)
                     /locus_tag="PKY63_06380"
     CDS             complement(2574..3119)
                     /locus_tag="PKY63_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00276.1"
                     /translation="MGAITFSAGFFSCFTGAGFLAGALTATTFLVAVFLTGAAFFTGV
                     LESFIFFATVFFTGVDFFATVLLADVFFLDTVFLTGADFLAGAFFIGVLLAEAGFLGA
                     VFLASGFTETDFLGAVLAGVDFLADALTGAAFFTTLFFAGTVFLTGAAFFAGAAFLEA
                     VLEEADFFLSVVFVLLTAISI"
     gene            3109..4116
                     /locus_tag="PKY63_06385"
     CDS             3109..4116
                     /locus_tag="PKY63_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPF00277.1"
                     /translation="MAPKSMAVAAPQSRNKQNTTVKTKEPVKSVEKKEEVKKTPTVKE
                     NAKKTVVESEQKMEHPVKPSAKKTESKKESNPEPVYTPFPEGSLEKSVRLKLEALYSL
                     QVIDTQIDKIRIIRGELPLEVQDLEDEIAGMNTRLDNYNAEIAAIDKSKVEYTEEIAR
                     SKEMIKKYEQQQKNVRNNREFESLAKEIEFQDLEVQLRNKKIKEADVAREAKQKLIDE
                     LRKALDAKEANLKEKMNELSEIIAETEKEEELLLKKSEKAKAIIEERYLNAYNRIRKS
                     VRNGLAVVKIERHSCGGCFSKIPPQRQMEIRMHKKVLVCEYCGRIIVDESISDAVDKQ
                     N"
     gene            complement(4172..5644)
                     /locus_tag="PKY63_06390"
     CDS             complement(4172..5644)
                     /locus_tag="PKY63_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00278.1"
                     /translation="MKKIIIIALGLLMTLNVFAQNETDALRYSFLMNSGTARFSAMGG
                     AFSALGADFSVLSTNPAGIGVYKSSEIMLTPQISFSNSSSSYLGTTTEDSKASFSIPN
                     FGMVFSGDISSSPEKAEWKNIQFGFGVNRLADFNNSYLMRGNNATHSILDVYQNLANG
                     YGHDELSSFDTDLAWNTYLLNPGSTDYDYIKAFEGGVLQQKSITTSGGINEMVMSFGG
                     NYNDKFYLGTTIGFPFLNYSEDTYYKETDDADTIGYFRSLSVNDQLNTHGSGVNFKLG
                     MIYRITDWVRISGAVHTPTFYAMHDTYSRVMRSDLDTANYESRSPQGVYDYRLTTPMR
                     INAGIAFVIGKMGAISAEYEMVDYSQSKLRARNAEYDFFDANEAINMNYTVSNNFKIG
                     GELKLNPFAIRAGYSYYGAPYAGTFAEDYSTSNITAGFGIREKNYYIDLAFVYTMKNE
                     SFDLYNASIIESMGMYLEPVLTDLSRTSVVITMGMKFGGK"
     gene            complement(5679..7421)
                     /locus_tag="PKY63_06395"
     CDS             complement(5679..7421)
                     /locus_tag="PKY63_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00279.1"
                     /translation="MKRTIYFLVFGAFVLLFSSCTVNYYTANPYDDVYYSRTDAAANV
                     TPDNYGNTADPANNTSAYVDETQPADYPEEKSYYDDNSQQYSTSETYTDENGTNYVTN
                     NYYGDYYDYEYSSRLRRFYGGYNGFDYYDPYYTNLYWYNYDPYYYGVSVYYTYSWWYR
                     PYRYSYWGWGWHYPYYHYYGWGHNNYWGWGNPYYSGYGWGYNNGYWNGYSDGYWNGYY
                     DGMYGNNYYYYNSYDYYSSNDTYYGPRGSSIGGGSNPRPETSGNNNSNISSLPASSFG
                     ARFENSLTAENINSNGLRVNTSKFVDRGNTSLSTNNANTARPNNESRVNTTNSTLNSG
                     NNATNNGTTTVSRPENNTNTTANPNVTRPNNNTNVNSGYTRPNNSGNTNTNSTYTRPN
                     NNTQTPVNNNSNSTQTRPSNSSSTQRPVQTYTSPSYSKPQSSNEYNSPSNNNYQRPGN
                     SSYQRPSTSGNSSNNSYSRPSNSGSNSSYSRPSNSGSTYSRPSNSGSQNTYSRPSNSG
                     SSSSTSRPSSSYSRPSNSGNSSSYSRPSSSSTSRPSSSYSSGSSRSSGSSSSGSSSGR
                     SSGSSSSSHSGGRR"
     gene            7487..8959
                     /gene="proS"
                     /locus_tag="PKY63_06400"
     CDS             7487..8959
                     /gene="proS"
                     /locus_tag="PKY63_06400"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPF00280.1"
                     /translation="MGSKITPRSENYSQWYLDIVQNAGLAENSAVRGCMVIKPYGFAI
                     WERMRDELDRMFKETGHQNAYFPIFIPKSFFSKEASHVEGFAKECAVVTHYRLKNASD
                     GSGVVVDEDAKLEEELIVRPTSETIIWDTYRTWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATEKEAVEEAERMLGVYSTFAEDWMAMPVLKGLKSANERFA
                     GAVETYCIEAMMQDGKALQAGTSHFLGQNFAKAFDVKFLSKENQLEHVWATSWGVSTR
                     LMGALIMTHSDDKGLVLPPTLAPIQVVLVPIFKNEEMKPKILEYLRGIEAELKEKKIV
                     VKVDDSENYSPGYKFAEYEMKGVPVRVAIGARDVENNTVEVARRDTLTKESAGREGIA
                     DRVFALLVDIQRNLYQKALELRKNLTSTVDTWEEFVDVIENKGGFVYAHWDGTTETEE
                     KIKELTKATIRLIPLEEGEPGKCVLTGKPSVKRVVFAKAY"
     gene            8961..9731
                     /gene="mazG"
                     /locus_tag="PKY63_06405"
     CDS             8961..9731
                     /gene="mazG"
                     /locus_tag="PKY63_06405"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPF00281.1"
                     /translation="MQRKLDEFRRLLEIMDRLREECPWDRKQTNETLRYLTIEEMYEL
                     GDAVLEKNDQEIMKELGDIMLHIVFYAKIGSEKGAFDMGDVLHSINEKLIRRHPHVFG
                     EVSVNDEEDVKANWEKIKMGEGRKSVLEGVPKGLPAVVKAFRMQEKVKGVGFEWERTE
                     QVWEKVQEELSELNEEVKTGAPVEKVEDEFGDVLFALVNYARFIGVNPENALERTNKK
                     FKERFTYIEQEAARMGRSLNDMTLAEMDAIWNEAKRKK"
     gene            9826..13410
                     /locus_tag="PKY63_06410"
     CDS             9826..13410
                     /locus_tag="PKY63_06410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPF00282.1"
                     /translation="MGKFIVIVALLLSFVLMTHAQTGADIDARVLSSQMADYNLVEPY
                     FKEAYELYPSVPKGMIEAVAFTNTRFYNVPSTEEASSEGLPRYLGVMGLIFDGKDYFN
                     ENGKLVATLSGVSEMLLLKPSARDQIIAWASAFDALSHQMGISGKKVENYVPVLIALS
                     EIPWHEGDQINAFAMNSHLYSVLSFLKDPYWAEICGFSTAAFDMNKIFAPNTLKILQS
                     EKVEISSKGVMDNKGNVMDGLGLKSTLSTDYAPAIEDLTPCNFSSRNGVAVSAYVLHT
                     VQGSYAGCISWFKNCSASASAHYVMRSSDGQVTQMVLEANKAWHVRDENPYTIGTEME
                     GYVSSASWYTEAVYVSHSNLVRDVCNSGYGINPLRTMYRDTLDDGTALDYGVHVLAGS
                     SYCTKIAGHQHYPNNDHTDPGNHWCWDYFYRKLNNSTPVTTYTTASGSFFDTGGSSAA
                     YSDDERKIWTIAPTNATSVTITFSSFDVEDNYDFLYIYNGPDVFSPRIGRYNTIAPTT
                     VTATSGVMTIEFRSDCATTAAGWVATWTSAAADATPPTTLVATTGLWKTDDFSATVSD
                     ADNTGGSGVQKGYYQVLENQGTEWRANASRGYFADNFDALHSDWTQTLGTWTIASGAL
                     HQTDTTQNNTNIYASLNQELSNRTLYHFTAMIPGTSNVNKRFGFHFFSDSAALDNRGN
                     GYFVFFRLETNQLEFYEVANNVFTQQMVVTNVVTNLNQWYDYKVIYDRITGEIWVYRD
                     DVLLGTWADTTPFTSGGKYISFRTGNAELKVTELKVYRSRASSVNVLVGTQATDDIRF
                     ENPSPSVYGAKIKSICQDVANNLSAIDYHDLNVDFTPPATIVSVNDGSAVDEDISYSS
                     SSLQANWTASADPNSDVSHYMIAAGTTAGGTDVLAWSNNGTALTATLNGLTLTQGSTY
                     YVSVKAVNNAGIEGAVTSSDGITIATSTLAGFFMSSNEICQGDSIQYTNSSVGATSYL
                     WEFTGGVPATSTLANPVVHYANSGVFGITLYAYGPTDTAEVMSPAAVTVRPLANAAFS
                     VNDTVFYIPDAMALFSNASVSAVSYAWDFGDGTTSVDVNPWHLYGDTGVYTVRLVAAS
                     EYCGNDTLNKTSYITVLFPDGIRGANANQLTVYPNPAGDFFEITRIADSREPALVLMY
                     NIDGQLVYSGSFVAGQQKLRITTTGFSGGLYQLVVSGNEILRVPLMVK"
     gene            complement(13422..14135)
                     /locus_tag="PKY63_06415"
     CDS             complement(13422..14135)
                     /locus_tag="PKY63_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF00283.1"
                     /translation="MRLTLVIAPLVVLLFLFRSSSAQEQGIENLPRFDLRTVHFGFTL
                     GFNNMDFTLRRPECNGPIDSVLNITTQAQGGFQIAIVSDLRLGSNMNLRFIPGLSFGD
                     RKVHYDVFHPSTGITTNVTKTAESTFLDFPLLFKYKTNRITNFRAYVLFGGKYSLDLA
                     SQSKKKQEDEANLKLLPHDYSVDLGVGVEWYLEYFKFGIELRSSFGIPDMLKRENTIY
                     TNNIESLRSKMVWLCFTFE"
     gene            complement(14283..16316)
                     /locus_tag="PKY63_06420"
     CDS             complement(14283..16316)
                     /locus_tag="PKY63_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPF00284.1"
                     /translation="MEDLQNDKPFELPENAYRELKEGETYEPVMPASKKFPETTTWSV
                     LMGLLMAVIFSAAAAYAGLKIGQVFEAAIPISIIAIGASALTKRKNALGQNVIIQSIG
                     ASSGVIVAGAIFTIPALYILQYKYPDIEIKFIEIFLAALLGGILGILFLIPFRKYFVK
                     EQHGKLPFPEAKATTEILISGEKGGKQAGLLITSGLIGGVYDFMAAQFRLWSDTVTTR
                     IIPWGAELAENTKMVFKMSTSALVFSFGYLIGLKYALIIAVGSMLSWLVFVPLFNEIG
                     NMMAAAGGGENIFAAMSPEAIFSGYVRNIGIGAIAMAGIIGIIKSSGVIKKSFGLAFS
                     GLSGKAKTEQPERTQKDIKMTFVLMFILLTVIAVFIFLYAGIQLTLVQALVALVTLVI
                     VSFLFTTVAANAIAIVGSNPVSGMTLMTLILSSVILVGVGLEGASGMVASLIIGGVVC
                     TALSMAGGFITDLKVGYWLGSTPAKQQSFKFLGTLVSAATVGIVIYILNGAYGFVKVP
                     GVHDDPMAAPQANAMASIIEPLMQPGASVPWMLFAFGAIISLLINWLGLPSLAFALGM
                     FIPLHLNTPLIVGGLLHKFMSSGHKDPEVNKAREQRGTLISSGFIAGAALFGVVGALL
                     LFFNVDLNFLHVNQMQINGSWDWIPGCPWPEVTALIAFAALIVYFVWDSKRIK"
     gene            complement(16413..16967)
                     /locus_tag="PKY63_06425"
     CDS             complement(16413..16967)
                     /locus_tag="PKY63_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00285.1"
                     /translation="MKIITLIAFVFTALIASAQHEKVLEIRNYYKDVKSHSEYAQPDS
                     ISYYSDQIIRNSGNMQWRAIGDFHDTLTFWYSDLMNAAYNNKNQNNDSTWALALVTSS
                     TQKSILNHYQEWLFKDGKLVFYFDKMMGYDEESTWEYRYYFNNNKLIRTMSGGQIIHF
                     DDAPQDVIKSSNEILHLFELLMRR"
     gene            complement(17047..18876)
                     /locus_tag="PKY63_06430"
     CDS             complement(17047..18876)
                     /locus_tag="PKY63_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF00286.1"
                     /translation="MKSFSRMLRLINGHWKNTIYYLVFNVLTALFSLFSIAMVIPFLR
                     VLFGMENPVTDPVSFELNLKSLQHNLNYFISEMLQNYGTDKTLIYVCFSVVILFLFRN
                     IFTYLSTYFMAPVRNGIVEDLRNKMYRKILHLPMSYFTNERKGDIMSRISNDVQEVDN
                     SVMSSIQVLFKDPIFIVIYLTSLFIMNYQLTLIILAMLPAIGFLLGIISRSLRKKARL
                     GQQRLGFLTSLIDETLHGLRIIKAFTAEETVYKNTAETNRKYTRLQIRIFRKRNLASP
                     LTEFVSILVMAIILYLGAKMVISDGMAFSAEAFIAYIMLFSQLLPPAKSFSNAWSSVQ
                     KGMASLDRVDELLNADEKILDPENPVEFKGFNESIEYVNVSFSYGNDDVLHNINLKIE
                     KGKTIALVGASGAGKTTMVDLLPRFFDVTDGEIRIDQKPVKHYNVLELRSLFGIVNQE
                     PILFNDTFFNNIAFGKPNATREEVEQAARIANAHDFIMETPQGYDSSIGERGSKLSGG
                     QRQRLSIARAVLKNPPILILDEATSSLDSENERMVQDALDRLLSDRTSLVIAHRLSTI
                     VKSDQIVVLDQGRIVEQGKHEELIALNGQYKHLYDLQMFGENN"
     gene            complement(18968..20188)
                     /locus_tag="PKY63_06435"
     CDS             complement(18968..20188)
                     /locus_tag="PKY63_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPF00287.1"
                     /translation="MTENSNLNLGPAGQLMPAEERLEIKRKKSSMKIGVPLEIAFQEN
                     RVSLAPDSVGLIVSHGHEVFVQRGAGRNAHFTDEEYAEAGAILTDSAAEIYQCNIILK
                     VAPMLPSEIDLLQSRQTVVSALHFTMQTTEYFRKLMQHKTTAVAFEFIKDQSNIFPVL
                     KAMSEISGYAAIQVAAEYLSKNDIGKGKLLGGIPGIAPVEVVILGAGTVGTYAARAAL
                     GFGAEVKVFDNHIYKLRTLQQTLGQQIFTSIIHPKVLSKSLKNADIVIGALHSIRNKP
                     MIVVNENMVADMKPGSVIVDVSIDQGGCIETSTVTTHRNPVFIKHNVIHYCVPNIPSR
                     VPQTASYGLSNFFTPVIMEMGEEGGIEAFLKNNPSLRQGIYMFNGILTNHIIGQNFGL
                     PSKELDLLLASFQM"
     gene            complement(20181..20615)
                     /gene="tsaE"
                     /locus_tag="PKY63_06440"
     CDS             complement(20181..20615)
                     /gene="tsaE"
                     /locus_tag="PKY63_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPF00288.1"
                     /translation="MKKEFSRVSLKDMILVADYLASLAEQHKVFAFYGPMGAGKTTLI
                     GKTIRILLGQELDVTSPTFALVNVYESKNPVYHFDCYRIEKPEDALNAGIEEMLYSNA
                     ICLVEWPEKIESILPQNVITVTLSTPDDESNRHIEINIPDHD"
     gene            complement(20619..22175)
                     /locus_tag="PKY63_06445"
     CDS             complement(20619..22175)
                     /locus_tag="PKY63_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HPF00289.1"
                     /translation="MNTKSKILWVDDEIEILKPHILFLEQKGYELTPVSNGNDALDLL
                     KTEDFDIIFLDENMPGLSGLETLTHIKNIKSGIPVVMITKSEEETIMEEAIGSKIADY
                     LIKPVNPNQILLTLKKNLDDRRLIQEKTTGSYQREFTKLGMEISPNLNFEEWSALYKK
                     LIFWEIELQESGDESMLQILQTQKEEANKVFSKAFLRNYREWLSDKNDPPLLSHNVMQ
                     KAVFPLLGKNEKVLVVVIDNLRYDQWKTIQPAIEEHFRVISDKMYCSILPTATQYARN
                     SLFSGLMPTEIQKKFPKLWIEDDDDENSKNQFEPELLDELLKRMGVNIKHNFTKILNL
                     RAGKKYVDTFSNIASNQLNVLVYNFVDMLSHSKTNMEIIKELANDESAYRSITSSWFD
                     HSPLAEVFELAAKNHFKIVLTTDHGSIRVKNPIKIYGDRETNANIRFKYGRNLNTEGK
                     NVFELKNPADLFLPRPNVSTSYMFCISTDYFVYPNNYNYYVNFFADTFQHGGISLEEN
                     LIPLITLAPK"
     gene            22299..23522
                     /locus_tag="PKY63_06450"
     CDS             22299..23522
                     /locus_tag="PKY63_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF00290.1"
                     /translation="MALNKRKILNDPVHGFITIPDELIFGLMNHPCFQRLTRIRQLGL
                     TYLVYPGALHTRFNHAIGAMHLMDEATEILKQKGFQISPDEKQAALAAILLHDVGHGP
                     FSHALEHSLIHNVSHEDISLRLMEQLNNEMNGQLRLAISIFKGTYRKKFLHQLVSSQL
                     DVDRLDYLSRDSFFTGVSEGVIGTERILKMMTIADDDLAVEEKGIYSVERFIIARRLM
                     YWQVYFHKTVVAAEQMLIGTLKRARELSFNGTDVFAPPALAFFLKQNQDQASLDALTL
                     GHFVNIDDSDVLSSLKVWQYHSDPVLSRLSANLLNRKLFRVTIDSKPISKKNEDVLLK
                     KIADSYAISKNDARYFIGYGEIENNAYAPGIDKIWILKKDGSKMDITEASDQLSQGSI
                     SKTVVKYFLCCPKEI"
     gene            23737..24354
                     /locus_tag="PKY63_06455"
     CDS             23737..24354
                     /locus_tag="PKY63_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPF00291.1"
                     /translation="MEAINMIQNRRSVNHFDAARKLDNETIRQIINLAVNAPSANNLQ
                     PWRIIVIESEAAKEKLWNIAFKQQKVKDAAVNVLVVGNKNGWDKNNPVWDEMLQTVGG
                     NKDIVEASQKGAAMGNGSTEERRIAFAQSNSGLLAMSIMYAASALGVDSHPMNGFDYA
                     AVQKEFGLAEHEMPVMNICLGYFDQSKTLHPRRPRRGFDEIAKVL"
BASE COUNT         7000 a         4694 c         5730 g         7077 t
ORIGIN      
        1 ggaatgattg gtatggagat ttttattgaa gaaaaaaaat aaaaaaaccg ctcacggatt
       61 tccatgagcg gctttcatta ttctgaaaca aacttatttt acaatcagtt tgatggtggc
      121 tgagctgcca tcatttccgg taatcataac atggtaaata ccagcttcca tttccgaaac
      181 atctatgctg gcctgctggc tgaaaatact cgaatataca acgcgtccgg tcatgtcaac
      241 aactttcagt atgccttctg cttcagctga aacagttaca aaatcactgg cagggttggg
      301 atataccgat gacgaaattt ctgtttcatc aatggcattg taaatggtta catcggctgc
      361 cgaacggggc tggatttttc tttcactgta gttgtaatcc ataacgcctg tgatgtcata
      421 atgtgttcct aatacaggaa cagaggtgta aataaggtcg cctactttca aggcatccat
      481 tccgtcacca acaacccatt cattgtagct gtcaggcata attgtgcatt cggcattggt
      541 cactttacaa agtacgcctt cgtacatttc ttcggctccg tcaggaatgt taatgttggt
      601 tacctgaact gggttgccac tgctgacttt aacatagctg cttaccgatt tcaattcagt
      661 aagattaaaa tattcatcaa caacagcaga gaatgttaca ctgtctccaa cagttggtga
      721 attgcctgaa tcatacacat aaacaccgct ccatggacca gatccgctct gaatccagta
      781 ggctccgctt ttcacggcgc tgacaatacc accggtatta accggttgtc caagatatga
      841 gcttgcatct gttgttgtgt actgaatgtc atatatagat acattgctca gtgtgccaac
      901 tgttacataa aatgagtcaa cctgcaggtg atcattggtg gcagaggtgt tccttactga
      961 cagaatgaat tctgctgttg ctgttgtcgt gtcacaaatg atactctgtt ctattttcgt
     1021 ccatgtggtg ccgtcaagaa ttacatatga gttgtatgtg tacgaagaac caccgtcgta
     1081 aagaccggtt ctgatttctc cgctgccacg ggcatagaaa ctgatgtcgt aaacagttcc
     1141 cgcagtaaga ctgacaggtt gtgtggtaac acgtttgtga gttgttgtgt cattgtttat
     1201 ctgcatggaa taagttcctg cataagggtt tgtggtgtat tggtcaacat ctgtgtacgt
     1261 gatgcttgtg gctgatccaa tccagttggt tggagtgttt gcatcaaccc aggtttcgaa
     1321 gtcgctgtaa aacaccgttt gtccgatgcc gcttccgatt gtaagtacaa acactgcggc
     1381 taaaagtaaa gcttttttca taacagtaaa aattttttca aaggtaagga atattttttt
     1441 agtaggtttg tattctgata tttgtcatgc tatgaaatgt aaagaattgt ttaaacggat
     1501 tgaggatagc gtcccgcttt catggcagga aagctatgat aattctggcc tgcagattgg
     1561 aaatccggaa aatgaagtgg agggcgtttt atattcactt gatgtaactc cttttgcggt
     1621 cgaaaaagcc gttaaagaag gatgtaatct tattattagt caccatccat ttttttttca
     1681 tggactgaaa agcatagatt tggctaatgt tactggtggc attatcgaaa cttgcattcg
     1741 tgaaaaaatt gccatatact ctgctcatac caattttgat aagcatccat cgggtgtaag
     1801 tgctgctctg gcaaatgcaa taggcttaga gaaacctgaa atactgtctc ccgagaatgg
     1861 catgttgaaa aaactggtca cctattgtcc cgaagcacag gccgaatctg ttcgcaatgc
     1921 cttattcagt gctggtgccg gacatatcgg aaattacgat tcgtgcagct tcaatgcgtc
     1981 cggactaggg tcattccggg ctggtgaaaa cacaaatccg ttcgttggag gaaaagggaa
     2041 agttcatttc gaacccgaaa tcagaattga atcagttttt cccaaagaca aacagactgc
     2101 agtgctgtct gccctgtttg catttcaccc gtatgaagaa gttgcatttg atatattttc
     2161 actcgataac caatattccg aatgcggtct tggtattgtt ggaaatttgt cgcagccatt
     2221 gtctgccaat gattttattt ctttcttgaa aacaagatta aatattcaga ctgttcggtg
     2281 taatactaat gccgtcgctc aaatctctcg tgttgccgtt tgcggtggtt ccggggcttc
     2341 gttgataaac actgccgtta gggcaggagc tcaggcatac attactgccg acctgaaata
     2401 tcatgatttt caggccgctg atggcagaat ttttcttatt gatgccggac atttcgaaac
     2461 cgaaattttt gctttaagtg cattaaagtt acttgtttcg gaaattttgc ctaattttgt
     2521 tcctcaaatt atttccccgg actcaaactg ggttaataca gtataaatca aattcatatt
     2581 gatatggctg taagcaaaac aaaaaccacc gacaaaaaga agtctgcttc ttctaagacg
     2641 gcttctaaaa aagcagctcc tgcaaagaaa gctgctccgg ttaaaaagac tgttccggcg
     2701 aaaaaaagtg tagtgaaaaa agctgctccg gtcaaagcat ctgccaagaa atcaactccg
     2761 gctaaaacag ctcccaaaaa gtctgtctcc gtgaaaccgc ttgccaaaaa gacggctcct
     2821 aaaaaacctg cttcagccag taaaactccg ataaaaaaag cgccggccaa aaaatctgcg
     2881 cccgtcaaaa aaacggtgtc caaaaaaaat acgtcggcta ataaaacggt agcaaaaaaa
     2941 tcaacgcctg taaaaaagac agtcgcaaaa aaaataaaac tctccaaaac tcctgtaaaa
     3001 aaagctgccc ctgttaaaaa aacagcgacc aaaaaagttg tcgcagttaa agctccggcc
     3061 aaaaaacctg ctcctgtgaa acatgagaaa aaacctgcag aaaaagtgat ggctcccaaa
     3121 tcaatggccg tcgccgcacc tcaaagtcgt aacaaacaaa atacaaccgt aaaaacgaaa
     3181 gaacctgtga aaagtgtcga gaagaaagaa gaagtgaaaa aaacaccaac tgttaaggaa
     3241 aatgcaaaaa aaacagtggt tgaatctgaa cagaaaatgg aacatccggt aaaaccttcg
     3301 gcaaaaaaaa cggagagtaa aaaggaaagc aacccggaac cggtttatac acccttccct
     3361 gaaggtagtc ttgaaaaatc agtccgtttg aaactcgaag ctttatattc gcttcaggtt
     3421 attgatacgc agattgacaa aatacgcatt attcgtggtg agcttcctct cgaagttcag
     3481 gaccttgagg atgaaattgc cggtatgaac acacgcctcg ataattacaa tgctgaaatt
     3541 gctgctattg ataaatccaa ggttgaatat accgaagaaa ttgcccgtag caaggaaatg
     3601 attaaaaaat atgaacaaca acagaaaaat gttcgtaata atcgcgaatt tgaatcgctt
     3661 gccaaggaaa tcgaatttca ggatctggaa gttcagttga gaaataaaaa aatcaaagaa
     3721 gctgatgttg ctcgagaggc caaacaaaaa ttgattgatg agcttcgtaa agcccttgat
     3781 gccaaagaag caaatctgaa ggaaaaaatg aacgagcttt ctgaaattat cgcggaaacg
     3841 gagaaagaag aagaattact gctcaaaaaa tcagaaaaag caaaagcaat tattgaagaa
     3901 cgttatctga atgcttataa ccgaattcgc aaaagcgtac gcaatgggct tgctgttgta
     3961 aagattgaaa gacattcttg tggtggttgt ttcagcaaaa ttccacctca acgtcagatg
     4021 gaaatccgca tgcacaaaaa agtgctcgtt tgtgaatact gcggcagaat tatcgttgat
     4081 gaatctattt cggatgctgt tgataaacaa aattagttga aaaaaaaaga gagatgtaaa
     4141 atctctcttt tttttgtttt ttcttttgct tttatttccc gccaaatttc attcccatgg
     4201 taattacaac actggttctt gataggtctg ttagaacggg ttcaagatac atgcccattg
     4261 attcaataat ggatgcgttg taaagatcaa agctttcgtt tttcatcgtg taaacaaagg
     4321 ccaggtctat gtagtagttt ttttctcgta ttccaaaacc ggcagtgata ttgctggttg
     4381 aataatcttc agcgaatgtt cctgcatatg gcgcaccgta atacgaatat cccgctctga
     4441 tggcaaatgg attcagtttg agttcaccgc caattttaaa attgttcgaa actgtataat
     4501 tcatattaat ggcttcgttg gcatcgaaga agtcatactc agcgtttctt gcacgtagtt
     4561 tcgactggct gtaatctacc atttcatatt ctgctgaaat agctcccatt tttcctatta
     4621 caaatgcaat cccggcgttt attctcatgg gcgttgtaag acggtaatca taaactccct
     4681 gcggagatct cgattcataa ttggctgtgt caagatccga tctcattacc cggctatatg
     4741 tgtcgtgcat tgcgtaaaaa gttggagtgt gtactgcgcc ggaaatccgc acccagtctg
     4801 taatccgata aatcataccc agtttgaaat tcacccccga tccatgtgtg ttcagttggt
     4861 cattgaccga tagagatctg aaatagccaa tggtgtctgc atcgtctgtt tctttgtaat
     4921 aggtgtcttc gctgtagttc aaaaacggaa aaccaatggt tgttcccaga tagaatttgt
     4981 cattgtaatt tccaccgaaa ctcataacca tttcattaat gcctcctgaa gttgttatgc
     5041 ttttttgttg cagtactccg ccctcaaatg ctttgatata atcatagtct gtgctgccgg
     5101 gattcaacag ataggtgttc caggcaagat ctgtatcaaa agaacttaat tcatcatgtc
     5161 catacccatt ggccaggttt tgataaacat caagaattga atgtgtggca ttgtttccac
     5221 gcattagata gctgttgttg aaatctgcaa gccggtttac gccaaatccg aattgaatgt
     5281 ttttccattc tgctttttcg gggctgctgg aaatgtctcc tgagaaaacc attccaaagt
     5341 tgggtatgga aaacgatgcc ttgctgtctt cggttgttgt tcccaaatag gaagaggaag
     5401 aatttgaaaa agagatttgt ggagtgagca ttatttccga tgatttataa acaccgatac
     5461 ctgccggatt ggtacttaat actgaaaaat ctgcacctaa tgctgaaaat gctccgccca
     5521 tggccgaaaa tcgggccgtt ccactgttca tcaggaaaga gtatcttaat gcatcggttt
     5581 cattttgtgc aaatacgttg agggtcataa gaagaccaag cgctatgatg ataatttttt
     5641 tcataacatc aattatttct ggttaaacaa gaagcttatt atctacgtcc tccgctatgg
     5701 ctgctgctgc tgctgccgct gctgcgaccc gaggatgatc ccgaggatga tgatcctgaa
     5761 ctgcgtgatg atcctgatga gtagctgctg cttggacgtg acgtgctgga gctgctggga
     5821 cgcgagtaac tgctgctgtt gcccgaattg ctgggacgcg agtagctgct gcttggacgt
     5881 gacgtgctgg agctgcttcc tgaattgctg ggccttgaat aggtattctg acttccggaa
     5941 ttgcttgggc ggctgtaggt actacctgaa ttgcttggcc ttgagtatga gctgttggag
     6001 ccggagttcg aaggtcttga ataactgttg ttggaagagt taccagaggt tgatggcctc
     6061 tgataacttg aatttcctgg tctttgataa ttattgttac tcggactgtt gtactcgttg
     6121 cttgactgtg gtttgctgta actgggtgaa gtgtaggtct gaacaggacg ctgcgtagaa
     6181 gagctgtttg aaggtcttgt ttgcgtagag tttgagttgt tgttgacagg tgtttgcgtg
     6241 ttattgttcg gacgtgtata ggttgagttt gtgtttgtgt taccactgtt gttcgggcgt
     6301 gtgtaacctg agttcacatt tgtattgttg ttcggacgag taacatttgg atttgcggtt
     6361 gtgttggtat tgttttccgg gcggctgaca gtagttgtcc cattgttggt tgcgttgttg
     6421 ccactattca gggtggaatt tgttgtgttt acgcgacttt cattgttggg gcgagctgtg
     6481 tttgcattat tggtagaaag agaggtgttt ccgcggtcaa caaattttga agtgttaact
     6541 cttaatccgt ttgagtttat gttttcagcg gtgagactgt tttcgaagcg ggcaccaaaa
     6601 cttgaagcag ggagcgaaga gatgttgctg ttgttgttgc cgctggtttc cggtctgggg
     6661 ttcgatccgc cgccaatgct gcttccgcgg gggccgtagt aggtgtcgtt gcttgaatag
     6721 taatcgtagc tgttgtagta gtaatagttg ttaccataca ttccgtcata gtatccattc
     6781 cagtatccgt cgctgtaacc gttccagtat ccgttgttat atccccagcc gtagccggaa
     6841 tagtaaggat ttccccatcc ccaatagttg ttgtgtcccc agccatagta gtgatagtac
     6901 ggatagtgcc atccccatcc ccagtaagag tatctgtagg gtctgtacca ccatgagtag
     6961 gtataataaa ctgacacgcc ataataatat gggtcataat tataccaata gaggttggtg
     7021 taataagggt cgtaatagtc gaaaccatta tatccgccat agaatctacg cagacgtgat
     7081 gaatattcat aatcataata gtcaccatag taattgttgg ttacgtagtt ggtgccattt
     7141 tcatcggtgt aggtttcgct tgttgaatac tgctggctat tatcgtcgta atagcttttt
     7201 tcttcagggt agtcagcggg ttgggtttcg tcaacgtatg cagatgtgtt atttgctgga
     7261 tcagcggtgt tgccgtagtt gtcaggagtg acattggctg cggcatctgt tcttgaataa
     7321 tatacatcgt cataggggtt tgctgtatag tagtttactg tgcagctgga gaaaagcagg
     7381 acgaaagctc caaataccaa aaaatagatt gtccttttca tttctttgtc ctccgaattt
     7441 tatttagtac ttttgttttt gaaataaaac caaatatcat accaaaatgg gtagcaagat
     7501 tacgccaaga agtgaaaatt attctcagtg gtatctcgat atcgttcaaa acgctggatt
     7561 agccgagaat tcagcggtcc gaggatgcat ggtaattaag ccatatggtt ttgccatatg
     7621 ggaacgtatg cgagatgaac tggaccggat gtttaaagaa acagggcacc agaatgcgta
     7681 ttttccaatt tttataccaa aatctttttt ttcgaaagaa gcaagccatg tggagggttt
     7741 tgccaaagaa tgtgccgtag tgacacacta tcgcctgaaa aatgcatcgg atggttctgg
     7801 tgtagtggtt gacgaagacg caaagctgga ggaggaactg attgtgcgtc cgacctcgga
     7861 gacgattatc tgggatacat acagaacatg gattcagtcg taccgtgatt taccattgct
     7921 gatcaatcag tgggccaatg tggttcgctg ggaaatgcga acgagattgt ttttgcgtac
     7981 agcggagttt ttatggcagg aaggccatac ggcccatgca acagaaaaag aagctgttga
     8041 agaagctgaa cgaatgcttg gcgtttattc aacatttgct gaagattgga tggccatgcc
     8101 ggtattgaaa ggtttgaaat cggccaatga gcggtttgca ggagcagtgg aaacttattg
     8161 tattgaggca atgatgcagg atgggaaagc gttacaggca ggaacttcgc attttttagg
     8221 gcagaatttt gccaaagcat ttgatgtgaa atttctgagc aaggaaaatc agctggagca
     8281 tgtatgggct acatcctggg gtgtttcgac ccgattgatg ggagcattga ttatgaccca
     8341 tagtgatgac aaaggtctgg ttttgcctcc aactctggct ccaatacagg ttgtgcttgt
     8401 tccgattttc aaaaatgagg aaatgaagcc gaaaattctg gagtatctga gaggaattga
     8461 agcagagctg aaagagaaaa aaatcgtcgt taaggtggat gacagtgaaa attattcgcc
     8521 gggatacaaa tttgcggaat atgagatgaa aggcgtgcct gtgcgggttg caatcggcgc
     8581 cagagacgtt gaaaacaata ctgttgaagt ggccagacgt gatacgctga ccaaagagtc
     8641 agccggacgt gaaggcattg ctgatcgtgt ttttgctttg ttggttgata ttcagcgcaa
     8701 tttatatcaa aaagctctgg agttaaggaa gaatctaaca tctacagtag atacttggga
     8761 agaatttgtt gatgtgatag aaaacaaagg cggatttgtg tatgcgcatt gggatggcac
     8821 aactgaaaca gaagaaaaaa taaaagaact cacaaaagca accataagac tgattccgct
     8881 ggaagaaggt gaacccggaa aatgtgttct tacaggaaag ccatcggtga agagagtggt
     8941 gtttgcaaag gcctattgat atgcaaagaa aacttgatga attcaggcgg ctgcttgaaa
     9001 ttatggaccg gctaagggaa gaatgtccct gggaccgcaa acagaccaat gaaacgttgc
     9061 gatatctgac cattgaagag atgtatgagc ttggcgatgc cgtacttgag aagaatgatc
     9121 aggagattat gaaagagttg ggcgacatta tgctgcacat tgtgttttat gcaaaaatag
     9181 gatcggaaaa aggagcattt gatatgggcg atgtgttgca tagtatcaat gaaaagctaa
     9241 tcagacggca tccgcatgtt tttggtgaag tgagtgtgaa tgatgaagaa gatgtaaaag
     9301 ccaattggga aaaaataaaa atgggtgaag ggcgaaagag tgtgcttgaa ggagttccaa
     9361 aaggtttacc tgctgttgta aaggccttcc gaatgcagga aaaagtgaaa ggtgttggtt
     9421 ttgagtggga aaggactgag caggtgtggg aaaaagtgca ggaagaattg tctgaattga
     9481 atgaagaagt taagactggt gcacctgttg aaaaagtaga ggatgaattt ggtgatgttt
     9541 tgtttgcatt ggtgaattat gcccgtttta ttggcgtaaa tcctgaaaac gcacttgaaa
     9601 gaacaaacaa gaaattcaaa gagcgattta catatattga acaggaagct gcccgcatgg
     9661 gccgttcgct gaatgacatg actctggcag aaatggatgc catctggaac gaagccaaaa
     9721 gaaagaagta gaaaatattt cacattgatt tttgattata attactgaaa acacaaaatg
     9781 gtttcggtat aagtttttat ttttgtactt taaaatgata tgattatggg gaaatttatt
     9841 gttattgtcg cattattgct ttcttttgtt ttaatgactc atgcacagac aggtgcagac
     9901 atagatgccc gtgttttaag cagccaaatg gcagactata atcttgttga gccttatttt
     9961 aaagaagcat atgaattata tccatccgtt ccgaagggaa tgattgaagc tgtcgccttc
    10021 acaaataccc gtttttacaa tgtgccttcg acagaagaag cttcgagtga aggtttgcct
    10081 agatatctgg gagtgatggg acttattttt gacgggaagg actattttaa tgagaatggg
    10141 aagctggttg caacattgag tggggtaagc gagatgttgc tactgaagcc gtcagcacgc
    10201 gatcagatta ttgcatgggc ttcggctttt gatgcattgt cgcatcaaat gggcatatcc
    10261 gggaaaaagg ttgaaaatta tgttccggtt ttgattgccc tgagtgaaat tccctggcat
    10321 gaaggcgatc aaatcaatgc atttgccatg aattcgcatt tatattcggt tttaagtttt
    10381 ttgaaagatc catattgggc agaaatttgc ggattcagca ctgcggcatt tgatatgaat
    10441 aaaatttttg cacccaatac tttgaaaata ctgcaatcgg aaaaagtaga gatttcttca
    10501 aagggcgtta tggataataa aggaaatgtg atggatggac tgggtctgaa gagcactttg
    10561 tcaacggact atgcgcctgc cattgaggat ttgacgcctt gtaatttcag ttcaagaaat
    10621 ggtgtagccg tttcggctta tgtcttacat acagtgcagg gatcatatgc cggatgcatc
    10681 agctggttca aaaactgttc ggcttcggcc agtgcgcatt atgttatgcg cagtagcgat
    10741 gggcaggtaa cccagatggt tttggaagcc aacaaagcat ggcatgtgcg tgacgaaaat
    10801 ccatatacca tcggaacgga aatggaagga tatgtgagca gtgcatcgtg gtacaccgaa
    10861 gccgtttacg tcagtcattc caatctggtt cgcgatgtct gcaacagcgg atatggaatt
    10921 aatccgttgc gcacgatgta tcgtgacact ttggatgatg gaactgcgct tgattacggt
    10981 gttcatgtgc ttgccggttc gagttattgc acaaagattg ccgggcatca gcattatccc
    11041 aacaatgacc acaccgatcc tggaaatcat tggtgctggg attattttta cagaaaactg
    11101 aataattcga cacctgttac gacatataca acagcttcgg gaagtttttt tgataccggt
    11161 ggatcatcgg ctgcatacag cgatgatgag agaaaaatat ggactattgc accaaccaat
    11221 gccacttcgg taactataac tttttcttcg ttcgatgtcg aggataatta tgattttctg
    11281 tatatttata acggaccgga tgttttcagt ccgcgcatcg gacgatataa tacgatagcg
    11341 ccgacaacgg ttactgctac aagcggagtg atgaccattg agttcagaag tgattgcgct
    11401 accaccgcag ccggatgggt agccacatgg accagtgccg ctgcggatgc cacaccccct
    11461 accaccttgg ttgcaacaac cggattgtgg aaaactgatg atttttctgc aaccgtttct
    11521 gatgctgata acactggtgg aagcggcgtt cagaaagggt attatcaggt tcttgaaaat
    11581 cagggaacag aatggcgggc caatgcttcg cgcggatatt ttgccgataa ttttgatgca
    11641 ctgcacagtg attggacaca gacgctcggc acctggacta ttgcatccgg tgcgctgcat
    11701 cagaccgata caacgcaaaa caatacaaat atctatgctt cattgaatca ggaactatcc
    11761 aatagaacac tgtatcattt tacagccatg attcccggaa cttcgaatgt gaacaagcgt
    11821 tttggatttc attttttcag cgacagtgcg gcacttgata accgaggcaa cggatatttt
    11881 gtgtttttca gattggaaac caatcagctt gagttttatg aagtagccaa caatgtcttt
    11941 acgcagcaga tggtggttac aaatgttgta accaatctga atcaatggta tgattataaa
    12001 gttatttatg atcgcattac tggtgagata tgggtttatc gtgatgatgt attgcttgga
    12061 acatgggcag atacaacccc attcacaagc ggtggaaaat atatttcgtt ccgaaccgga
    12121 aatgcggagt tgaaagtaac agaactgaag gtttatagaa gccgggcaag ctcggtcaat
    12181 gtgctggtcg gtactcaggc aacagatgac attcgttttg aaaatccatc tccatcggta
    12241 tatggagcaa aaataaaatc gatttgtcag gatgttgcaa ataatttatc tgctatagac
    12301 tatcatgatc tgaatgttga ttttacgcct ccagcaacca ttgtaagtgt aaatgatggc
    12361 agtgctgtcg atgaagatat ttcgtacagc tcatcatcgt tgcaggccaa ctggacggct
    12421 tcggcggatc cgaattcaga cgtgtcgcat tacatgatag cagccggaac cacagcagga
    12481 ggcaccgatg tacttgcgtg gagcaataat ggaacagcgc tgacagcgac attgaacgga
    12541 ctaaccctga cacagggatc gacatattat gtttcggtaa aagcagtgaa caatgcagga
    12601 attgagggtg ccgttacaag cagtgatgga atcaccattg ccacgtcaac gttagccgga
    12661 tttttcatga gttcgaatga aatctgtcag ggcgacagta ttcaatacac caacagtagt
    12721 gttggagcta catcgtattt atgggaattt actggtggag tgccggccac atcgacattg
    12781 gccaatccag ttgtgcatta tgcgaacagt ggagttttcg gaattacgct ctacgcctat
    12841 ggtccgactg atacagctga agtaatgtca ccagctgctg ttacagtgag gcctttggca
    12901 aatgcagcat ttagtgtgaa tgacacggtt ttttatattc ctgatgcgat ggctttgttt
    12961 agcaatgcaa gtgtgagtgc agtttcctat gcatgggatt ttggagatgg aacaacgagt
    13021 gttgatgtta atccatggca tttgtatggc gataccggcg tttatacagt tcgacttgta
    13081 gctgcatcgg agtattgcgg aaacgacacc cttaacaaaa cttcgtacat cacagttctt
    13141 ttccctgatg gaatcagagg tgcaaatgca aatcagctga cagtatatcc gaatccggcc
    13201 ggtgattttt ttgaaatcac acgtattgct gatagtaggg agcctgcatt ggttttaatg
    13261 tacaacatag acgggcagct ggtttacagc gggtcatttg ttgcaggaca gcagaaattg
    13321 cgtattacca ctaccggatt ttcaggtgga ttgtatcaat tggttgtgag cgggaatgag
    13381 attttgcggg taccgttgat ggtgaaataa tcccgagttg attattcaaa tgtgaagcac
    13441 agccacacca ttttggagcg gagcgattcg atattatttg tgtatatggt gttttcacgt
    13501 ttgagcatgt ctggaattcc aaaagaagag cgcagctcaa ttccgaattt aaaatactcg
    13561 agataccatt caacacctac gcccaggtca acagaataat catgtggcag cagtttgaga
    13621 ttggcttcgt cttcctgttt cttttttgat tgagaagcga ggtctaggct gtatttgccg
    13681 ccaaaaagaa cgtatgcgcg gaaattggtg attcggtttg ttttatattt aaacagcagc
    13741 ggaaaatcga ggaatgtgga ttcggctgtt tttgtaacat tagtggtgat gccggttgat
    13801 ggatgaaaca cgtcgtaatg aactttgcgg tcaccgaatg aaaggccggg aataaatcgc
    13861 aggttcatgt tactgcccag gcgaagatca gaaacaatgg caatctgaaa tccgccctga
    13921 gcctgagttg taatattgag tacagaatcg atggggccat tgcattcagg ccggcggagt
    13981 gtaaagtcca tgttattgaa cccgagggta aaaccgaaat gcactgtcct cagatcaaag
    14041 cgcggcaggt tctcgattcc ctgttcctga gcagatgatg agcggaaaag gaaaagaaga
    14101 acaaccagtg gagcaatgac gagcgtaagg cgcataaaag aattacggtt tattgcttaa
    14161 taactgcaaa agtaagaata tattattaat atgctttttg caaaagcaat catggtgatt
    14221 caaacccgca cagaaacagc gaatactgta aaaaacgcaa aaccaactta ttggcctgaa
    14281 tactatttaa ttcttttgct gtcccagaca aaataaacaa ttaaagcagc aaaagcaatc
    14341 aatgctgtta cttcaggcca tgggcaaccc gggatccagt cccagcttcc gttgatctgc
    14401 atctggttaa catgcaggaa attgagatcg acattaaaga agagtagtaa tgccccgact
    14461 actccgaaaa gagccgcgcc cgcaataaaa ccgcttgaaa tgagcgttcc gcgctgttcg
    14521 cgggctttat tgacttcggg atctttgtgt ccgcttgaca tgaatttgtg cagaagaccg
    14581 cctacaatca gcggtgtatt gaggtgcagc ggaataaaca ttccgagtgc aaatgccagc
    14641 gaaggcagtc caagccaatt gataagaaga gaaataatgg caccgaaagc aaacagcatc
    14701 cacggtactg aggcacccgg ctgcataagc ggttcgatga tagaagccat tgcattggcc
    14761 tgtggagctg ccatgggatc atcgtgaaca cccggaactt ttacaaaccc gtaggcaccg
    14821 ttaaggatgt aaataacaat gccaaccgta gcagcagaga ccaatgtacc aagaaattta
    14881 aacgactgct gtttggcagg ggttgatccg agccagtacc cgaccttcaa gtcagtaatg
    14941 aaaccgccgg ccatggaaag ggctgtacag accacaccgc caatgatgag tgaggcaacc
    15001 atcccggaag ctccttcgag tccaacccca accagaatga ctgaagaaag aatgagcgtc
    15061 atcagtgtca taccggaaac ggggttggaa ccaacaatgg cgattgcatt ggcggcaacc
    15121 gttgtgaaca gaaatgaaac aataaccaat gtaaccagcg ctacaagcgc ttgtacgagt
    15181 gtcagctgaa tacctgcata tagaaagatg aatacagcaa tgacagtcag cagaataaac
    15241 atcagcacaa aagtcatttt aatatctttc tgtgtccgct ccggttgttc agtttttgct
    15301 tttcctgata gtccggagaa tgcaagaccg aaagattttt tgatgacgcc agacgatttg
    15361 ataatcccaa tgataccagc catagcaatg gcgccgattc cgatgttgcg tacatatccg
    15421 gaaaaaatgg cttcgggcga catggccgca aaaatatttt caccgccgcc ggcagcagcc
    15481 atcatgtttc cgatttcgtt gaaaagcggc acaaacacaa gccaagaaag catggatccg
    15541 actgcgatta tgagggcgta tttaagtcct atcagatatc cgaagctgaa cacaagtgcg
    15601 ctggtgctca ttttgaaaac catttttgta ttctcggcca actcggcgcc ccaaggaatg
    15661 atgcgggttg tgactgtgtc ggaccacaat ctgaactgag cagccataaa atcatagaca
    15721 ccaccaatga ggccgctggt gatgagcagc ccggcctgtt ttccaccttt ttcgcccgaa
    15781 atcagaattt cggttgtggc tttggcttca gggaaaggaa gttttccatg ttgttctttg
    15841 acaaaatatt tccggaaagg aatcaggaac agaataccga gaatgccacc taacagggcg
    15901 gccagaaaaa tttctatgaa tttgatttca atatcggggt atttgtattg cagaatgtat
    15961 aaagctggaa tcgtgaatat ggctccggca acaataactc cggagctggc tccgattgat
    16021 tgaataatga cattctgtcc aagtgcattt ttgcgtttgg taagagctga tgctccaatg
    16081 gcaatgattg aaatggggat tgcggcctcg aacacctgtc cgattttcag gccggcgtat
    16141 gctgcagcag cagaaaaaat aacagccatc agaagtccca tcaggactga ccatgtggtt
    16201 gtttcaggaa atttcttcga agccggcatt accggttcat aggtttcacc ttctttgagt
    16261 tcgcggtatg cgttttcggg gagttcaaat ggtttgtcgt tctgcaggtc ttccataatt
    16321 tcaagattta acagattggg agtgcaagat agttaaagtt tgaaatatga taaaacagaa
    16381 attaaaacaa cagtaagttt gctgtttgtg ttttagcgtc tcatcagcag ttcaaataaa
    16441 tgcaggattt cgttggatga tttgatgaca tcctgcgggg catcatcaaa atgaatgatc
    16501 tgtcctccgc tcatggtacg gataagtttg ttgttgttga aataatagcg gtattcccag
    16561 gttgattctt catcgtatcc catcattttg tcgaagtaaa aaacaagttt cccgtcttta
    16621 aacaaccatt cctgataatg attcagaatg cttttttgtg tcgatgaagt taccagagcg
    16681 agggcccagg tgctgtcatt gttttgattt ttgttgttgt atgctgcatt catcagatcg
    16741 ctgtaccaga aagtcagggt gtcgtgaaag tcgccaatgg cgcgccattg catgttgccg
    16801 gaatttcgga taatctgatc ggaataataa gagatggaat caggttgcgc atattcggaa
    16861 tgacttttta catctttgta ataattacgt atttctagca ctttttcatg ttgtgctgaa
    16921 gcgatcaatg cagtaaaaac aaaggcaata agagtaatta ttttcattct catattgttc
    16981 gattaaaaat ttgatttcaa agtcaaatgt acaaattttg caaatgaatt gagagagagg
    17041 ggggtgttaa ttattttccc caaacatttg caaatcgtac aaatgcttgt attggccgtt
    17101 taaagcaatc agttcttcat gttttccctg ttcaacaata cgaccctgat cgaggacaac
    17161 aatctgatcg gattttacaa tagttgagag gcggtgagca ataacgagtg atgtgcggtc
    17221 gctcagaagg cggtcaagag catcctgaac catgcgttca ttttcggagt cgagcgaaga
    17281 tgtggcttca tcgagaataa ggattggagg gtttttgagt acggcacgag caattgaaag
    17341 tcgttgtcgt tgaccaccac taagtttgct gccacgttcg ccaatggatg agtcataccc
    17401 ttgcggagtt tccataataa aatcatgagc attggcaatt cgtgcggcct gttcaacttc
    17461 ttcgcgagtg gcattgggtt tgccgaaagc aatgttgttg aaaaatgtat cattgaaaag
    17521 tatcggttcc tgattaacga tgccaaaaag actgcgtagt tcaagtacat tgtagtgttt
    17581 taccggtttc tggtcgattc ggatttcacc atccgttacg tcaaaaaagc gaggcagcaa
    17641 atcgaccatg gtggtttttc ctgcaccgga agcgcctacg agagcaatgg tttttccttt
    17701 ttcgattttg aggtttatgt tatgcagaac atcgtcgttg ccataggaaa aagaaacatt
    17761 tacatattcg atagattcat tgaatccttt gaattcaaca ggattttcag ggtcaagaat
    17821 tttttcatcg gcattgagaa gttcgtcaac gcgatcaagt gaggccatgc ctttttgcac
    17881 actagaccag gcattggaaa agcttttggc ggggggcaat aattgcgaaa aaagcataat
    17941 ataagcaatg aaagcttcgg ccgaaaatgc cattccgtcg gaaatgacca ttttagctcc
    18001 aagatagaga atgatggcca ttacaagaat agaaacaaat tcagtgagtg gagaagcgag
    18061 attgcgtttt ctgaaaattc tgatttgaag tcgtgtgtat tttcggttgg tttcagctgt
    18121 attcttataa acggtttctt ctgctgtaaa agcttttatt attcttaaac catgtaatgt
    18181 ttcatcgatg agcgaagtga gaaatccgag tcgttgctga ccgagtctgg cttttttgcg
    18241 cagcgagcgg ctgatgattc ccagcagaaa gccaatagcc gggagcatag ccagaataat
    18301 gagggtgagt tgataattca ttataaacaa gctggtgaga tatatcacga taaaaatcgg
    18361 atctttaaac aaaacctgaa ttgaagacat gacactgttg tcaacttcct gtacatcgtt
    18421 tgaaatgcgg ctcataatgt cacctttacg ttcattggta aaatacgaca taggaagatg
    18481 cagaattttt cggtacattt tgttgcgcaa atcttcaaca attccattgc gcaccggagc
    18541 cataaaataa gtggacaaat aagtaaaaat atttctgaac aagaaaagaa taaccaccga
    18601 gaagcaaacg tatatcagtg ttttgtcggt tccatagttt tgcagcattt ccgaaataaa
    18661 ataattcaga ttgtgttgca gtgatttcag gttgagttca aatgaaacag ggtctgttac
    18721 cgggttttcc atcccaaaaa gcacacgcag aaaaggaata accatggcaa tggagaagag
    18781 agaaaagagg gcagtaagaa cattgaacac cagataataa atggtgtttt tccagtgacc
    18841 gttgatcaga cggagcatcc gggagaaact tttcatgcga atggatttga attcaggaca
    18901 aagatataaa aattgtggca ggcgattttt acgcacaccg atggattttt atttcttggt
    18961 tgaagtatta catctgaaaa ctggccagca gcagatccaa ttccttgctt ggcagaccga
    19021 aattctgtcc gatgatgtga ttggtcagaa tgccattgaa catgtagatg ccttgccgca
    19081 acgatggatt atttttcaga aatgcttcta ttccgccttc ttcgcccatt tccatgatga
    19141 caggagtgaa aaaattgctg agtccgtacg aggcagtttg cggaactctt gatggaatgt
    19201 tgggtacaca ataatgaatc acattgtgtt tgatgaaaac cggattacga tgtgtggtaa
    19261 ctgttgatgt ttcaatgcac cctccctggt cgatgctgac atcgacaatg acagagccgg
    19321 gtttcatgtc ggcaaccata ttttcattaa caacaatcat gggtttgttg cgaatggaat
    19381 gcagtgctcc aataacaatg tctgcgtttt tcagtgattt tgacaatact ttcgggtgaa
    19441 taatggaagt aaaaatttgt tgtccgagtg tctgctgcag tgtacgaagt ttgtagatat
    19501 gattgtcgaa tactttaact tctgctccaa agccaagtgc tgcgcgggct gcataagtgc
    19561 ccactgttcc ggcaccaaga ataacgacct caacaggagc aataccggga atacctccaa
    19621 gcaatttacc ttttccaata tcgtttttgc tgagatattc ggctgccacc tgaatcgcgg
    19681 catagcccga aatctcgctc atggctttca gtacaggaaa aatattactc tggtctttaa
    19741 tgaattcaaa tgcaacagca gttgttttgt gttgcatcag ttttctgaaa tattcggtgg
    19801 tttgcatggt aaaatgcaaa gccgagacaa ctgtttgcct gctttgcagc aagtcaattt
    19861 cgctgggcag catgggtgct actttgagaa taatattgca ttgataaatt tcagcagcgg
    19921 aatcggtgag aatggcacca gcttcggcgt attcttcatc ggtgaaatgt gcattgcgac
    19981 cggctccacg ctgaacaaaa acttcgtggc catgcgaaac tatcagaccg acactatcgg
    20041 gagccaatga aacacggttt tcctgaaagg caatttcgag cggcactccg attttcatgc
    20101 tgctcttttt tctcttgatt tccagccgct cttcagccgg catcagttgt cctgcaggac
    20161 ccagattaag attgctgttt tcagtcatga tcgggaatat ttatttcgat gtggcggtta
    20221 gattcgtcat caggtgttga aagtgttacg gtgattacat tttgaggaag aatggattcg
    20281 attttttcgg gccattcgac cagacaaata gcattgctgt ataacatttc ttcaattcct
    20341 gcattcagcg cgtcttctgg cttctcaatg cgataacaat cgaagtggta aacagggttt
    20401 tttgattcat atacattgac caatgcaaag gttggactgg taacatccaa ttcttgtcct
    20461 aataaaatgc ggattgtttt gccgatgagt gttgtttttc cggctcccat ggggccgtaa
    20521 aatgcaaata ctttgtgctg ttcagctagc gaagccagat aatcggcaac gagtatcata
    20581 tccttcaatg aaacgcggct gaattctttt ttcatgtttt attttggcgc aagagtgatg
    20641 agtggaatca ggttttcttc gagcgaaatg ccgccgtgct gaaatgtgtc agcgaaaaaa
    20701 ttcacgtaat aattgtagtt gttgggatac acaaaataat cggttgaaat gcagaacatg
    20761 tatgatgtgc ttacatttgg acgagggaga aataagtcgg ccggattttt cagttcgaag
    20821 acatttttgc cttcagtgtt gagatttctg ccgtatttaa aacgaatatt ggcattggtt
    20881 tcccgatcgc cataaatctt tatcggattt ttcacacgta tgcttccatg atcggttgtc
    20941 agtactattt tgaaatgatt tttggcggca agttcaaaaa cttctgccag cggactgtga
    21001 tcgaaccaac tgctggtaat ggaacggtag gccgattcat catttgccag ttctttaatg
    21061 atctccatat tggtttttga gtgcgaaagc atatcaacaa aattgtacac aagcacattt
    21121 agttgatttg atgctatatt gctgaaagtg tcaacatatt ttttcccggc tctgagattc
    21181 agtattttgg tgaaattgtg ttttatattc acacccatgc gtttgagcag ttcatccagc
    21241 aactcgggtt caaactggtt ttttgagttc tcatcgtcat catcttcaat ccagagtttg
    21301 gggaattttt tttgtatttc ggtaggcatc aggcccgaaa acaatgaatt tctggcgtat
    21361 tgtgttgccg tgggcaatat actgcaatac atcttgtcgc tgattactct gaaatgttct
    21421 tctatggcgg gttgtatggt tttccactgg tcgtatcgca aattatcaat gacaacaacc
    21481 aacacttttt cattttttcc cagcaagggg aaaacagctt tttgcatgac gttgtgcgaa
    21541 agaagtggtg gatcgttttt gtcgctgagc cattcccggt aatttctgag aaaagctttt
    21601 gaaaacactt tgttggcttc ttctttctga gtctgaagaa tctggagcat gctctcatcg
    21661 ccggattcct gtaattcgat ttcccagaaa ataagttttt tatacaatgc tgaccattct
    21721 tcaaaattca ggttgggaga gatttccata cccagttttg taaattcacg ctggtatgat
    21781 cctgttgttt tttcctgtat cagacggcgg tcgtccagat tttttttcaa tgtcagcaaa
    21841 atctgatttg ggttaacggg cttaatcagg tagtctgcaa ttttgctgcc gatggcctct
    21901 tccatgatgg tttcttcttc gcttttggtg atcatcacca ccggaatgcc ggattttatg
    21961 ttttttatgt gcgtcaaagt ttccagtccg ctcaatccgg gcatgttttc atcaagaaaa
    22021 ataatgtcaa aatcttctgt ctttaacaga tccagcgcgt cgtttccgtt cgatacggga
    22081 gtgagctcat atcctttttg ttccagaaac agaatatgtg gtttcagtat ttcaatttca
    22141 tcatcaaccc agaggatttt gctttttgta ttcatgtttt tttagttttc cggttattca
    22201 ttacatttgt ctgttttttt gacagggtgg tttgtcgtaa gcaaaatcat tttcaaagat
    22261 acaaaaataa tttgcaaaaa acattgaatt ttctgacaat ggctctcaac aaacgcaaaa
    22321 tactcaacga tccggtgcat ggttttatta cgattcctga tgaattaatt ttcggattga
    22381 tgaatcatcc gtgttttcag cgtttgacgc gtatccggca gcttggactt acgtatctgg
    22441 tttatcccgg tgctttgcat acccgcttca atcatgctat tggtgccatg catctgatgg
    22501 acgaagctac cgaaatttta aaacagaaag gatttcagat ttcgccggat gaaaaacagg
    22561 cggctcttgc tgctatcttg ctccacgatg ttggtcatgg cccgttttcg catgcactcg
    22621 aacactcgct gattcataat gtgtcgcacg aagatatttc tctccgcctg atggaacaac
    22681 ttaataatga gatgaacgga cagctccggt tggcaatcag tatctttaaa ggcacgtaca
    22741 gaaaaaagtt tttacatcag ctcgtttcaa gtcagcttga tgtcgatcgg cttgattatc
    22801 tttcccgcga cagttttttt accggcgttt ctgaaggtgt tattggaaca gagcgcatac
    22861 tgaaaatgat gaccatagcc gacgatgatc ttgccgtcga agaaaaggga atttattcgg
    22921 ttgaacgttt cattattgcc cgccggctaa tgtattggca ggtgtatttt cataaaactg
    22981 ttgtcgcagc cgaacaaatg ctgataggga cattgaaacg cgcccgggaa ctatctttta
    23041 atggaaccga tgtgtttgca ccaccagcgc ttgcattttt tttaaaacaa aatcaggatc
    23101 aggcttccct cgatgcatta actcttggac attttgtcaa tatcgatgac agcgatgtgt
    23161 tgtcatcgct caaagtatgg cagtatcaca gcgatcctgt tctcagccgc ttatcggcca
    23221 atctgctcaa tcgtaaatta tttcgtgtta ccattgattc aaaacccatc agtaaaaaaa
    23281 acgaggatgt tctgctgaag aaaattgctg atagttatgc tatttcgaaa aatgatgccc
    23341 gttattttat tggttatggc gaaattgaaa ataatgcata tgctccgggt atcgataaaa
    23401 tctggattct gaaaaaagat ggcagtaaga tggatattac tgaagcttcg gatcagttga
    23461 gccagggctc gatttcaaaa accgtggtga aatatttcct gtgctgccct aaggaaatat
    23521 aaaagtgaaa agtttataaa gttcatcaag tggaaaggca aggttttgag agttaggagc
    23581 aagtgctcag cctttacctc gacctggaca cgcgtctccg cttgcaacgt ttcacgttga
    23641 cgcgtgccct ctctctcatg ataatcatca tatcgaatct gcacttttca gtcctgaacc
    23701 atcgtatctt tgtaaaaaca taaaatcaaa aattctatgg aagcaatcaa tatgatacaa
    23761 aaccgtcgct cagtgaatca tttcgatgcg gcacggaaac tggacaatga aacaatcaga
    23821 cagattatca atcttgcggt caatgcacct tcggcaaaca atcttcaacc atggagaatt
    23881 attgttattg aaagtgaagc agctaaggaa aaactctgga atatcgcatt caaacagcaa
    23941 aaagtgaagg atgctgcggt gaatgttttg gtggttggaa ataaaaacgg atgggataaa
    24001 aacaatccgg tgtgggacga aatgctgcaa actgttggag gaaacaaaga tattgttgaa
    24061 gcgtcgcaaa aaggagcagc tatgggcaac ggttctaccg aagagcgccg cattgctttt
    24121 gcgcagtcaa acagtggttt gctggctatg agtattatgt atgctgcatc ggcgcttggg
    24181 gttgattctc atccaatgaa tggctttgat tatgcagctg tgcaaaagga atttggtctg
    24241 gccgaacacg aaatgccggt catgaatatt tgtcttggct atttcgatca aagcaaaaca
    24301 cttcatccgc gccgcccccg aagagggttt gatgagattg cgaaagtact ttgaagcaca
    24361 atgctttgca agtttatcaa gtttgtaaag tggaaaggtg acgtatacgc ttgcggcatg
    24421 aggcatgcgg agaataaagc ttaagactta taccaaagtg atttataaat cggttcaatt
    24481 atttttggga gactccggag g
//
LOCUS       DAOPKE010000037        24416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2169_length_24416_cov_11.532244, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000037 DAOPKE010000000
VERSION     DAOPKE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_24416_cov_11.532244"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1502)
                     /locus_tag="PKY63_06460"
     CDS             complement(129..1502)
                     /locus_tag="PKY63_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF00292.1"
                     /translation="MKNILILLILFLLTAGRSGAQNADSVMIKNIFDNALQSEVSYLQ
                     LKGLCESAPGRLAGLPAMDTAMQYMFRLMKTMPFDSVYVQPCMVRQWLPGRDTAYYTV
                     DGKRNHPLTVDILGGSVPTPERGITAQVVIFNSMDEMKKADPSNIKGKIVYFNRPMNQ
                     THVNTFRAYGENASMRVLGADEASKLGAVAVLVRSINIATDDFPHTGIMRYKTTDPKI
                     PAFAISTKDALELDSVLMKFPETNVYLHSTCKELPEIQQGNVIGEIRGTVHPERFITV
                     GGHLDAWYNSAGAHDDGGGCMQSLEVARLFFATGYKPQHTLRVVMFIDEEMDQRGGKK
                     YAEEALLKSEKHLFALESDRGVALPTGFSIDAPEAAYNRIVSWKNLLEPYGFYLWYKG
                     GSGVDVGFLKGNCTALAGIVCDSQRYFEYHHSANDTFDKIVRREMQLGAAGMAALIYL
                     VDKYGLE"
     gene            complement(1514..2257)
                     /locus_tag="PKY63_06465"
     CDS             complement(1514..2257)
                     /locus_tag="PKY63_06465"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HPF00293.1"
                     /translation="MSLINRIFRKPELIGYSVIETDIHSHYLPGVDDGCKETEESLLL
                     LKRMYECGFRKIFCTPHIQSEYYRNSRENILPVFDKLRDAVSDAIPGLELEVAAEYLI
                     DEGFERKVKDGLIFLGNNKHVLVELSYFNPFPKFRNVLQDILMKGYTPVLAHPERYLY
                     WPVEESVFEDLHAAGVLFQVNLPSICGYYGPEVRKRAFDMIEKGFVSLAGSDIHNVHY
                     ADAVCDGLNNKKVQDILKSGVFKNSEIFG"
     gene            complement(2244..3236)
                     /locus_tag="PKY63_06470"
     CDS             complement(2244..3236)
                     /locus_tag="PKY63_06470"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF00294.1"
                     /translation="MKTGSIFSKIKGKKCIVTGGAGFIGSHITDALLSHGAARVIVID
                     NLLTGSIENIKHHFDNDAFTFAQADINDANTIAAHFIKADVVFHQAALGSVPRSVENP
                     PATDINNVHGFVNILHLCRLNKVKKVVFASSSSVYGNDQTMPKTEEKTGVPLSPYAAS
                     KVADELYGRVFADLYGMSITGLRYFNVFGPRQNPKGPYAAVIPLFIDHVLKKESVRIF
                     GDGEQSRDFTYVSNVVTANVLSAFVLDEIGFNVMNIGCGGSTSVNELFNAINEAAGGK
                     SKASHEPERPGDIRSSSASIARATKLIGYKPETTIDDGIRKTIEWFKKNKNVFD"
     gene            complement(3223..4278)
                     /gene="rfbB"
                     /locus_tag="PKY63_06475"
     CDS             complement(3223..4278)
                     /gene="rfbB"
                     /locus_tag="PKY63_06475"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPF00295.1"
                     /translation="MKKTIIITGGAGFIGSHVVRLFVNKYPDCRIVNLDKLTYAGNLE
                     NLRDIEKKENYVFVKADICDFDRINEIFNEYKPDGIVHLAAESHVDRSIINPTEFVMT
                     NVMGTVNLLNAAKNLWKDNMDGKKFHHISTDEVFGSLGETGLFTETTAYDPRSPYSAA
                     KASSDHFVMAYHHTYGLPTVITNCSNNYGPYHFPEKLIPLMINNIIHNKPLPVYGQGL
                     NVRDWLYVEDHAAAIDLVFHKGKCGETYNVGGNNEWKNIDLVYLLCDIMDMKLGREKG
                     TSIQLITYVKDRAGHDLRYAIDSSRIQKELGWEPSVTFEEGLEKTVEWYLENQEWLDR
                     ITSGDYMNYYKDMYENR"
     gene            complement(4275..5300)
                     /gene="galE"
                     /locus_tag="PKY63_06480"
     CDS             complement(4275..5300)
                     /gene="galE"
                     /locus_tag="PKY63_06480"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPF00296.1"
                     /translation="MKKILVTGGLGYIGSHTVVELINAGLDVVIADDLSNSEESVLER
                     IFITTGTGPEFHKMDVGNRNELNKLPEDIDGVIHFAAFKSVGESVEKPLEYYRNNIGS
                     LVSVLDFCRERKIQSFVFSSSCTVYGQSEQLPVTETAPFLPAWSPYGHTKQMGEEILN
                     NFVQSKPGIKVVSLRYFNPAGAHELAFIGELPRGVPNNLMPFITQTAMGLRDKLKVFG
                     GDYNTKDGTAVRDYIHVSDLAQAHVKALEYSEKMLVNNIEVFNLGSGNGFTVLEVIQS
                     FEKTSGLKLPYDIVDRRSGDIEKVWADTTKAEAVLGFRPSRTLDEITLSAWKWEQNYR
                     NKIQKKS"
     gene            complement(5302..6600)
                     /locus_tag="PKY63_06485"
     CDS             complement(5302..6600)
                     /locus_tag="PKY63_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPF00297.1"
                     /translation="MKTYEKLLNHETKLSVIGLGYVGLPIALEFAKTIDVIGFDIKPE
                     RVELMKKNIDPSDEIASEEFLNKRIVYTSKPEDLKDATFHIVAVPTPIDNYNLPDLTP
                     LVKASETVGKILKKGDTVVFESTVYPGATEEDCIPIMEKFSGLRFPQDFNVGYSPERI
                     NPGDKVHTLTNTVKIVSGNTSEVLEEVAKVYELVIKAGVHRAPSIKVAEAAKIIENTQ
                     RDINIGLMNELSIIFGRMGINTLDVLEAAGTKWNFLKFQPGLVGGHCIGVDPYYLVHK
                     AQEYKYHPQIIRAGRFVNDSMGSYVAKQCVKKLIKAGRNILDSKVLVMGVTFKENVTD
                     IRNSKVVDIINELISYGVNVDAVDPHADAKQFEHEYGLPLASGPQGKYDAVILAVMHE
                     AYMDMNEDKIASMLKDEKGVLIDVKGILRHHIKKLTYWSL"
     gene            complement(6610..7563)
                     /locus_tag="PKY63_06490"
     CDS             complement(6610..7563)
                     /locus_tag="PKY63_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF00298.1"
                     /translation="MSNPMNFALIGAAGYIAPRHMKAIRDTGNRLTALLDPYDGIGVI
                     DSYFPDADYFSEPERFDRHLDKMRRAGESKIDYVSICSPNYLHDAHIRMAMRNQADVI
                     CEKPLVLNPWNIDALAEIENETGKRINNILQLRLHESIRHLKEETEKRSNEFFDVDLT
                     YITSRGHWYHHSWKGDESKSGGVATNIGIHFFDMLTWIFGSVEQSIVHMHSDQKAAGF
                     FRLKNANVRWFLSIDENDLPEEIKGQKRTFRSIRVNGSEFEFSEGFTELHTDSYKQIL
                     AGNGFGLEDARRSIEMVYNIRNASIAPLTGEFHPILNKINQ"
     gene            complement(7563..8135)
                     /locus_tag="PKY63_06495"
     CDS             complement(7563..8135)
                     /locus_tag="PKY63_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPF00299.1"
                     /translation="MADFFAHPTSVIDENCTIGEGCKIWHFSHLMSGCVLGKGCNIGQ
                     NVVVSPEVILGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNISNPRSAIIRRDQYEQ
                     TLVKKGASIGANATIVCGNTIGEYALIGAGTVVTKDVLPYALIIGNPGKQMGWVSEYG
                     HRLIFREDGTAECPESGEQYVLKNGIVTKK"
     gene            complement(8137..9267)
                     /locus_tag="PKY63_06500"
     CDS             complement(8137..9267)
                     /locus_tag="PKY63_06500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPF00300.1"
                     /translation="MKPIRMVDLQGQYEKIKSEIDNSIMEVVSSTAFINGPAVQRFQK
                     SLEQYLGAKHVIPCANGTDALQVCMMALGLQPGDEVITSSFTFIATAEVIALLHLTPV
                     LVDVEKDTMNISIDAIRRAITPKTKAIVPVHLFGQSAPMEEIMALAKEFNLYVIEDAC
                     QSIGSDYYFNDGSIKKTGAIGHLGCTSFFPSKNLGCYGDGGAVFTNDDALAEKLRIVV
                     NHGMKIRYYHDEIGVNSRLDSIQAAVLDVKLKYLDNYIKARIAAADYYDKAFANHPKL
                     ITPFRYKKSRHVFHQYTLVGQGIDRNALIAFLQERGIPCMIYYPVPLHMQKAYLDPRY
                     KQGDFPNTEWLSQNVFSLPMHTELDEEQLEFITGSVLEFLNK"
     gene            complement(9365..10018)
                     /locus_tag="PKY63_06505"
     CDS             complement(9365..10018)
                     /locus_tag="PKY63_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00301.1"
                     /translation="MPLYNKLRVVFLALTALFITSCIELEERIKIYPDKSGDYSLKLD
                     MGALTQSGMDAFKPSEDILNFPKTVENAVKDVVGITDVKTISDTKKGLYQVSFHFKNH
                     KVFKKALLQLAGMKYGFVVPKYMKVGKHRFSKKNIGPLIAKQINKQEDSPLLQDFMGV
                     EVSSMINVKTIIETPHEVKKVKKNARAVKSDDPEIVEIKSTLKDILNGSSTGVVVKF"
     gene            complement(10002..10718)
                     /locus_tag="PKY63_06510"
     CDS             complement(10002..10718)
                     /locus_tag="PKY63_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPF00302.1"
                     /translation="MKKYMIAFAAVLVMFTACDTLNKLNLPTNVPVVLSEGDVANGLK
                     EALNVGTNNAIGMLGKADGILKNPLFMIPFPQEAIKAKEKLQQLGMETLLDNFVATMN
                     HGAENAVAKAGPIFLNAITAMTITDAINILKGADNAATEYFKSKTTASLTALFKPEIT
                     KALDAVNCTKYWSDITGTYNKIPLVTPIETDLSKYVTEKALGALFLQIAAEEKKIRTD
                     PAARINDILKKVFDPNAIVQ"
     gene            10795..11937
                     /locus_tag="PKY63_06515"
     CDS             10795..11937
                     /locus_tag="PKY63_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPF00303.1"
                     /translation="MNLPEGSLISYFSTLVKQHGGINLAQGIPGFSPPQELLDELKNI
                     AHENIHQYPPGIGNFTLLEQLLAYFSDQFPLKKENMIIMQGATEALCLIFLYVMRQTK
                     GGFTTLSFTPPYESYHQLPKQFGLPFIELDPDENKQLPLAQIKEKIESDNVRLIFLAS
                     PGNPHGIMFSKESVNALIELCQQKECFLVFDSVYRTLYFETKPYVPLSKLNPYLFIVD
                     GFSKWFSITGWRVGFLIHDEAHCSSLRSLHDYTGLCANSVLQEALSRFIAKNNFGETY
                     SGNLRKILAANYQFASKKLDAMGFNCKKPDGGYFVWAELPETFSNGFEFAVKLFDYQK
                     VAVVPGIHFSEKAEKIVRFNIARPLPELTEGLEKISCFLKKNSEKT"
     gene            complement(11947..13095)
                     /locus_tag="PKY63_06520"
     CDS             complement(11947..13095)
                     /locus_tag="PKY63_06520"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPF00304.1"
                     /translation="MRSVILFLLVISFASCNTYKVIPVHNYTKLKKEGVFYYLPETRL
                     VVDITVKETHYLKGPFSQYSAKFTGISNVITENKTEYSIVGIALNSFSVPDKSKLFYI
                     ETKRLKEKEPFLLSVNEDGCIVSFNNPSDAGFEAADKSLLVENDRASQSMNVGFEHFA
                     TGNTQVKTDTIIEKIILDTITIEKQILQHSIIEKSLEDKAKDAADYIRLISENKMNLI
                     SGYQEVDYDETTFRSMLDELNKMQQEYLTLFTGKTVEYITHYRIVIDPEKPVDNDFIF
                     SFYQTEGVAEIEDSLFTDNMYYYQIMPEERTEAVVAAVSNTKRSANAGLAYNVPEKCF
                     FIIRNGNGEVVLMEKVSVCQLGTVLQLPSSIHQVLLNPKDGSIRRVEK"
     gene            13159..14250
                     /locus_tag="PKY63_06525"
     CDS             13159..14250
                     /locus_tag="PKY63_06525"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPF00305.1"
                     /translation="MDKKSTIRIGNNKSPLIISENPVMDFKRWWQLRIGTHKTTPVII
                     LVDRTVHSAYSVLIGDLLKNLPVSVIETRQIDAGENCKQWDNAGVILKEWINFPVTKD
                     TVIICMGGGTVTDLGGFIAAIYKRGLRTVYIPTTLMAMTDASLGGKNAINAGSDKNQL
                     GTISFPQFTLVSTMWLASLPAREIQSGYAEIIKHALLDDYSFVKTILDISDTSVPPPL
                     SLLVRSIRVKMKIVTDDPYETHERLWLNLGHSFGHAYESFFYATGNPVSHGHAVAIGL
                     AEALYFSTILLGFSKKIYAEVIIWLRQQFIIDSLPEWNEIRASVLKDKKNISSGIRLV
                     LLKSPGNPLLRSMDESTCQTLHADFLKQL"
     gene            complement(14311..16455)
                     /locus_tag="PKY63_06530"
     CDS             complement(14311..16455)
                     /locus_tag="PKY63_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPF00306.1"
                     /translation="MATIGKIRKHSGLLIGIIGVALALFVLSDFMTSSGGRRSAEPLA
                     EVFGEKVNYQDFSVMVEERKAQYMMQYGQEIQFTASDNFQISNEVFDKMVENLILQEE
                     YDKLGLAVSDAELGELFTGKFVHPLIRQLFTNPETGIFDPNQVAMYVESIEERSPEEQ
                     QNWHMYEKMIIEERTKNKYTTLVNKGYYLPKAFIERDNIEKNKKFVTTYTGLRYASIS
                     DSTITVTEEEMKKYYEEHKYDFISEELVAAVDFVIFDVKPSEADHKFMKEKVDTIYSR
                     FTKTADADIVGFINSNADIDFTWDSSYLRREVLPVKADTLFGANIGTFVEPYLENNIY
                     FMHKLLDRKMIPDSLKASHILIAYQGAQNADSTVVRTKEQAKQMADSLLLAVRGKDSV
                     FFSQAAIAHSNDPSVQQNAGYFDWFQEGMMVPEFNKACVDGAIGSYTVVETIFGYHVI
                     KVIDKTKPVNKVKIATIQRTVEPSNTTSDSIYNLANVFAAESKTQEAFEKNIIDRGYT
                     KRVADKVKTTEFTLPGIAEGREVVRWAYNEDTEINSVSPVFSLDTETKYVIAVLKSRS
                     EKGQASFESVKAIVEPLAKREKKAQMLMDKMNAALNGVTSVDALAKKLSVDTDTFDVT
                     FATYSLPGFGPESEVVGNITASSKGKLSKVIKGETGVFVFTVLDVVEAAPADHKVILS
                     QKMQFFQSKANYELFKALKNKAELEDNRILYF"
     gene            complement(16515..17057)
                     /gene="lptC"
                     /locus_tag="PKY63_06535"
     CDS             complement(16515..17057)
                     /gene="lptC"
                     /locus_tag="PKY63_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPF00307.1"
                     /translation="MAAMAVTMLFSCGNSMNSIKEVTVKDTLPVEQAMDVTMYYSDSG
                     HIEACLTAPVMKRFDNKDQKGVLKLTEGLKVIFYDSLGQEETVLTARYGERLDELQRI
                     DVKYDVVIITADSEKMYTDHLIWDERANRIYSNEFIKIITPDKIIWGDGFESDERFDN
                     YRIMRVKGEIEIKDDKNTSE"
     gene            complement(17098..18531)
                     /locus_tag="PKY63_06540"
     CDS             complement(17098..18531)
                     /locus_tag="PKY63_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00308.1"
                     /translation="MKTVLNFIFVFALLFSAIHVAKAQDSITFPKSCSIKGPKYGTDS
                     VNGVKNFSLYRENYKQWKDSRYKGDAIKYTVAPWRYCFNNAPLISQNLYLDGVRIVDY
                     MIETAADSASKERYIDTLMMVYDQNIEAFGCARLYGEGYILGRKGYDLYTFRPGEIEL
                     IYYTLEKSLKIQGNESEAAVLSIFYKVTDEMVRAGLLDTSMIYENYDIAMSAAEFQLS
                     EYRKELKEKPGDSLKINKKIEIYEIAENNINSLFDPWANCEQIIAINSAKFETKKSDL
                     NWLLKLTELMEKKNCTEDPLYFNAAEELYKQSPTPMSALALGKSFLKIKNFSSAAKYL
                     SEAVAGLEDPIQKAEAYLNLADAYRNMNQYANARNAALQSASLNPNDGRPYLIIGDLY
                     YSTVSSCGGDTPVSKKAGYWAAADKYAKAKSVSTDENVISVANARYNACYGGFPNSEE
                     LFFENITVGSSYTVSCWYTETTIVRSK"
     gene            complement(18544..19851)
                     /locus_tag="PKY63_06545"
     CDS             complement(18544..19851)
                     /locus_tag="PKY63_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00309.1"
                     /translation="MKIYFIGLILLVSSLQLKAQSFVLSPYSRYGLGEMQTSTEPVSF
                     SMGGLTAAFRDRYAVNSFNPASYTILLHDQDSFPMLFNGGFKGNFSNQTTASQTANRF
                     SGALNSFSFAFKAANNWGMAFGLQPYSSVGYNLEAAFPVDSLANYTAKYEGSGGFNKF
                     WMGHAIEVANHLSIGANVSYVFGSLSQTRRIVFDTTAYYNTKTVSSRYVGDVALDAGL
                     QYEIVLNRDSLTSRKTRLVLGAAVGLPVNLNAKESLLAMRYYASGTSEYIIDTTGVSD
                     NIAGTISLPMSISGGFMIAKDQNWSFGVDFKVQDWSGYTSFGESDSLKNSLSLNAGFS
                     YSPNNSAVSKYMKRVTWYGGFHYDQTYLSLKGEELSKIGISFGCSWPLKSIYPRLQRS
                     VITTGFEFGKMGTQSQNLMEEKYIRFVLGISLKERWFEKKKYN"
     gene            complement(19848..20585)
                     /locus_tag="PKY63_06550"
     CDS             complement(19848..20585)
                     /locus_tag="PKY63_06550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPF00310.1"
                     /translation="MYLYTVNLNLTIDIGNTRCKYAVFSDEKIIESGADFFSVDYIHA
                     VLKKYPGSRKIYASVVELTEEQQDFFRIQEIINVKSCMQVPVKSEYKTPETLGEDRWA
                     AVCGAAFLSNPAVPFMVIQAGTAITFDYVDERGYYVGGAISPGISLRLRALHNFTSRL
                     PLVEPENKFELMGMSTKDSIISGVMNGCLAEINYRIDEFLYRTSGGTVFIAGGDASYF
                     DISHENHIFAVPKIVLVGLNHLLNLNP"
     gene            20641..20713
                     /locus_tag="PKY63_06555"
     tRNA            20641..20713
                     /locus_tag="PKY63_06555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20674..20676,aa:Phe,seq:gaa)
     gene            complement(20759..21514)
                     /locus_tag="PKY63_06560"
     CDS             complement(20759..21514)
                     /locus_tag="PKY63_06560"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HPF00311.1"
                     /translation="MKKFTLLLVLMAITPAMFAKAYSELTITINENADFYVVIDGRQY
                     DSYAGSVVVSRIEHGRHGMEVFRESMTLNGIMVDRIFRGSVDIMPNKRMYGTIDRFGA
                     FVILRSENMIPATGYSHGYGHNHSNGHGYGHSNHYGSSNGHYGTYSYGSNAYMPMGMS
                     PDAFGMLLSSIRREAFDETRVRMAITAVSVSGVTSAQLRELMLMLTFDSNRLKLAKTA
                     YTFVVDKGNIFMINDAFTFSSNADEFYRYIGVW"
     gene            complement(21626..23800)
                     /locus_tag="PKY63_06565"
     CDS             complement(21626..23800)
                     /locus_tag="PKY63_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00312.1"
                     /translation="MKHKILLLALIFAPILALAQAPGCPSLEAGPSPHFLTNDTTICG
                     PSNLTLTADVFHVGYTNTYTVTSIPYAPPYPFTSGTAIPINQDDYFGPVVTLPFQFCF
                     FGQSYNQLVVGANGIITFDLTKANQYCSWSFSNSLPSTSLFKNSIFGAYHDIDPSVCG
                     TFRYGIVGAYPCRSFVMNFDHICHFSCNSLQSTLQVVLYEGTNVIEVHILNKPTCSTW
                     NSGNCVIGIQNAAGTTAFVPPGRNTGPWTATNEAWRFTPNGAPAYTISWFNGSSLVGY
                     NAQETVMVAQQDTFIAIAAYEYCDGTQLIIRDTVHVYLNTQDVSITTPDSVLCLGEST
                     TLTAAGGDAYVWSDGSVSPSVTVSPTTSGYYSVTGTYMGTCEAYDSIYITVNPPPVIQ
                     LSASPAQICSGDSTLLSASGAVSWEWASMTNTLAAFMVAPTASTTYSVTGTDINGCTG
                     SADVTVVVNAIPDIQLYASPPDGCEDLFVQFSANVNPSAAFYNWSFSDGTTSNLAMPS
                     KTFTNPGKYDAKLDVISVDGCENSKSELGIVDVYELPVSNFIADTTWVSMDNPVVTFT
                     DLSTLASTYYWDFSDFSSTNNYSAEANPVHTFSGPGDYIVWQTVYSENGCSDKSYTVI
                     HVELNIAFYIPNAFSPYNNDGINDVFKPSGIGIGLDEGTFSMHIYDRWGKQVFESSDI
                     NDGWDGKVDGNLTAPGLYSYRIEVRYGDGLWHVFNGKVFVLE"
     gene            23872..24336
                     /locus_tag="PKY63_06570"
     CDS             23872..24336
                     /locus_tag="PKY63_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF00313.1"
                     /translation="MRQKIVTQKQELLQIISGSKICFVAMVDSAGKPYNLPFNFGFDE
                     DYIYLHSGSDGKKMRILAANPNVCIAFSNSEELAYQSADVACSHFMKYRSVLVWGAVE
                     FVEDPEEKMAILNKFMKHYTGRDGFKYNTPAISNVKVFRVSTKNMSGKTYGF"
BASE COUNT         7252 a         5624 c         4526 g         7014 t
ORIGIN      
        1 cttaacctta accttaacct taaccttaac cttaacctta accttaacct taaccaggct
       61 gtttatattg acctcgatct ccccggctca tagtaagaga tgacggctga ttgctgacag
      121 ctaaatgctt attccagccc atacttatca accagataaa tcaacgccgc cattcccgct
      181 gcgccaagct gcatctcacg tcgcacaatt ttatcaaacg tatcgttggc cgaatgatga
      241 tattcgaaat aacgctgcga gtcgcataca atgccggcaa gcgccgtaca atttcctttc
      301 agaaaaccta catccactcc actaccgcct ttataccaca aataaaatcc atagggttca
      361 agtaaatttt tccacgaaac aatcctattg tatgccgctt ccggagcgtc aatcgagaaa
      421 ccggtcggta aggctacacc acggtcgctc tccaatgcaa acaggtgctt ttcgcttttc
      481 aacaatgctt cttctgcgta tttttttcca cctcgctgat ccatttcttc atcaatgaac
      541 atcaccacac gcaaggtatg ctgcggcttg taaccggttg cgaaaaacag tcgagccact
      601 tcgagcgatt gcatgcaacc accgccatca tcatgcgcac cggcggaatt ataccaggca
      661 tcgagatgtc cgccaaccgt tataaaccgc tcagggtgta cagtgccgcg gatttctcca
      721 atgacatttc cctgctgaat ttccggtaat tctttacagg tgctatgcaa atatacgttg
      781 gtctcaggaa atttcatcaa cacagaatcc agctccagtg cgtctttggt gctgatggca
      841 aatgcaggaa tttttggatc cgtagtttta taacgcatga tgccggtatg tgggaaatca
      901 tcagtagcta tattgatcga acgcaccaaa accgcaacag caccaagttt tgaagcttca
      961 tcggcgccca acacacgcat agatgcattc tcgccatacg ctctgaaagt attcacatgc
     1021 gtttgattca tcggtcggtt gaaataaaca atctttcctt ttatgttgga tggatctgct
     1081 tttttcattt catccatact gttgaaaatc actacctgag cggtaatccc gcgttctggc
     1141 gtcggcaccg atccgccaag aatatcaact gttaatggat gattcctttt cccatccaca
     1201 gtgtagtacg ccgtatcgcg cccggggagc cattgacgaa ccatgcatgg ctgaacgtac
     1261 accgagtcaa aaggcatggt tttcatcagg cgaaacatat attgcatcgc agtatccatg
     1321 gcaggcaatc ctgcaagacg ccctggagcc gattcacaca atcctttcaa ttgcagataa
     1381 gagacctcgc tttgcaatgc attgtcaaaa atatttttga tcatcacgga gtcggcattc
     1441 tgtgcgccgg aacgaccagc agtaagaaga aacaaaatca ataaaatcag aatatttttc
     1501 atgtatcatt gaatcagcca aatatttccg aattcttaaa aacgccgctt ttcaggatgt
     1561 cctgtacttt tttattattc aggccatcgc agactgcatc agcataatga acattatgaa
     1621 tatcgctccc tgcaagcgaa acaaatcctt tttcaatcat atcgaaagca cgcttgcgca
     1681 cttcagggcc ataataacca cagattgaag gcaaattcac ctgaaacaaa acgccggctg
     1741 catgcagatc ttcaaacacg ctttcttcaa caggccagta aagataacgc tcaggatgtg
     1801 ccaagacagg ggtataacct ttcatcagta tatcctgcag aacatttctg aatttcggaa
     1861 aaggattgaa ataagacaac tcaacaagaa catgtttgtt attaccaaga aaaatcaatc
     1921 catctttcac tttcctttca aaaccttcat caatcagata ctcagcggca acctccaatt
     1981 caagcccggg aattgcatca gaaacggcat cgcggagttt atcaaaaacc ggaagaatat
     2041 tctcacgact gtttcggtaa tattcagact gaatatgcgg agtacaaaaa atcttacgaa
     2101 aaccgcattc atacatgcgt tttaaaagca acaacgactc ttctgtttcc ttacaaccat
     2161 catcaacacc cggaagataa tgggaatgaa tatctgtttc gattactgaa taaccgatca
     2221 gttcgggctt ccggaatatg cgattaatca aagacatttt tgtttttctt gaaccattcg
     2281 attgtcttac gaatgccatc atcgatagtc gtctcaggtt tataaccaat caacttcgtt
     2341 gccctggcaa tgctggcaga cgaagaacgt atatctcccg gacgctccgg ctcatgcgat
     2401 gcttttgact ttcctccggc agcttcattg attgcattga aaagttcatt cactgaagtt
     2461 gaaccaccac aaccgatatt catgacatta aaaccgattt catccagcac aaaagctgaa
     2521 agaacattgg ctgtaacaac attgctgaca tatgtaaaat cgcggctttg ctcaccatcg
     2581 ccaaaaatcc ttacactttc ttttttaagg acatgatcga taaaaagtgg aatcactgcg
     2641 gcatacggac ctttcggatt ctgtctcgga ccaaaaacat tgaaatacct caagcccgta
     2701 atactcatcc cataaagatc ggcaaataca cgcccataca attcatcggc tactttactg
     2761 gcagcgtagg gcgacaacgg aacacccgtt ttttcttccg tttttggcat ggtctgatca
     2821 ttcccatata ccgaagatga cgaagcaaaa acaactttct tcactttatt caaacgacac
     2881 aaatgcaata tgtttacaaa accatgcacg ttattgatgt cggttgccgg aggattttca
     2941 accgagcgcg gaacgctgcc cagagccgcc tgatgaaaca caacatctgc ttttataaaa
     3001 tgagcagcaa tcgtattcgc atcgttgatg tcggcctgcg caaatgtaaa agcatcgtta
     3061 tcgaaatgat gtttgatgtt ttcaatactt ccggtcaaca gattatcgat aacaatcact
     3121 cgtgcagctc catgagacag caacgcatca gtaatgtgcg aacctataaa tcccgcacct
     3181 ccagtaacaa tacatttctt tcctttaatc tttgaaaaaa tactacctgt tttcatacat
     3241 gtctttataa taattcatat aatctcccga cgtaattctg tcgagccatt cctgattttc
     3301 caaataccat tcaactgttt tttccaatcc ttcttcaaat gtcacagagg gctcccagcc
     3361 cagttctttc tgaattctgg aagaatcaat tgcataacgc aaatcgtgac cagctctatc
     3421 tttcacatat gttatcaact gtattgaagt tcctttttcc cgacccaatt tcatatccat
     3481 aatgtcgcaa agaagataaa ccagatcaat atttttccat tcgttattgc ctccaacatt
     3541 atacgtttca ccacattttc ctttatgaaa aacaaggtca atagcagctg catgatcttc
     3601 cacatacaac cagtcgcgca cattcaaacc ttgaccgtaa actggaagcg gcttattgtg
     3661 aataatattg ttgatcatca gagggatcag cttttctgga aaatgatacg gaccataatt
     3721 gttgctgcag ttggtaatca ctgtaggcaa gccgtatgta tgatgatagg ccatcacaaa
     3781 atgatcactc gatgccttgg cagccgaata cggactgcgc ggatcgtatg ctgtagtctc
     3841 tgtaaacaga ccggtttcac caagagaacc aaaaacttca tcagttgaaa tatgatgaaa
     3901 ttttttacca tccatattgt ctttccataa gttttttgcc gcattcaaaa gatttaccgt
     3961 gcccatcaca ttggtcatta caaactcagt tggattgatg atgctgcggt caacatgcga
     4021 ctcagccgcg agatgcacaa tcccatccgg tttatactca ttgaaaattt cattgatacg
     4081 gtcaaaatca caaatatctg cttttacaaa cacataattt tctttcttct caatatcgcg
     4141 cagattctcg agattgccgg cataagtcag tttgtcaaga ttaactattc tgcaatcagg
     4201 atatttattt acaaacaaac gaaccacatg tgatccgata aatccagcgc cgccggttat
     4261 aatgatggtt tttttcatga tttcttctgt attttatttc tgtaattctg ttcccatttc
     4321 catgctgaaa gcgtgatttc atcaagcgtt cgcgatggtc taaaaccaag cactgcttca
     4381 gcttttgttg tatcagccca tactttttca atgtcgcccg aacgacgatc aacaatgtca
     4441 taaggcaatt tcaaaccact cgttttttca aaactctgaa taacttccag caccgtaaaa
     4501 ccattcccgc tgccgagatt aaaaacttct atattgttga caagcatttt ttcgctgtat
     4561 tccaacgcct tcacatgcgc ctgtgccaga tccgaaacat gaatgtaatc acgcactgca
     4621 gtgccatctt tcgtattgta atcgcctcca aaaactttca gtttgtcgcg taatcccata
     4681 gcagtttgcg taataaaagg catcaggttg ttgggtacac cgcgcggcaa ctcgccaata
     4741 aaagcaagct catgagcccc tgccggatta aaataccgga gcgatacaac ttttattcct
     4801 ggcttcgatt gaacgaaatt gttcagaatc tcttcgccca tctgcttggt atgtccatag
     4861 ggcgaccatg ccggaaggaa aggcgctgtc tcagttacag gtagttgctc actctgccca
     4921 tatacagtgc atgatgaaga aaaaacaaag gattgtattt ttctttctct gcaaaaatca
     4981 agaacactca ccaaggatcc aatgttgttg cggtaatatt caagtggctt ttcaaccgat
     5041 tcgcccacac ttttaaaagc ggcaaaatga atcactccgt caatgtcttc aggaagttta
     5101 ttcaattcat tcctgttccc gacatccatt ttgtgaaatt ccggaccagt gcctgtagtt
     5161 ataaaaatcc gctccagaac agactcttcg ctgtttgaaa gatcgtcggc tatcacaaca
     5221 tcgagtcctg cattgattaa ttcaacaacc gtatgcgagc caatatatcc caacccgcct
     5281 gtaaccaaaa tctttttcat actacaaact ccaataggtg agctttttta tatgatgacg
     5341 caaaatgccc tttacatcaa taagaacacc tttttcatct ttcaacatcg aagcaatttt
     5401 atcttcattc atatccatgt atgcttcgtg cataacagcc aaaatcacag cgtcgtactt
     5461 gccctgcggg ccagatgcaa gcggtaatcc atattcatgt tcaaattgtt ttgcatcggc
     5521 atgaggatca acagcatcga cattcactcc atacgaaatc aattcattga tgatgtcaac
     5581 cactttgctg ttgcggatat cggtcacgtt ttccttaaat gtaacaccca tcaccagcac
     5641 tttgctatcg agaatattac gtccggcttt aataagtttt tttacacact gcttcgcaac
     5701 ataagatccc atgctgtcgt tcacaaaacg accggcgcgg ataatctgcg gatgatattt
     5761 atattcctgt gctttatgaa caagataata cggatctact ccaatgcaat ggccacccac
     5821 aagacccggc tgaaacttga gaaaattcca tttcgtgcct gcagcttcca atacatcgag
     5881 tgtattaatg cccatacggc caaaaataat ggaaagttca ttcatcaatc caatgttgat
     5941 gtcgcgctgc gtgttttcaa tgatctttgc agcttcggcc accttaatgc ttggcgcacg
     6001 gtgcactccg gctttgatta caagttcgta cacttttgca acttcttcaa gaacttcaga
     6061 tgtattacct gaaacaattt tcacggtatt ggtcagtgtg tgtaccttgt cgcccgggtt
     6121 gatgcgctct ggcgaatacc cgacattaaa atcctgcgga aatctgagac cactgaattt
     6181 ttccataatc ggaatgcagt cttcttctgt tgcaccagga taaacagttg attcaaacac
     6241 aacagtgtcg ccttttttca gaatttttcc aacagtttca ctcgctttta ctaaaggggt
     6301 cagatcgggc aaattgtaat tatcaatggg cgttggaact gcaacaatat gaaaagttgc
     6361 atccttcagg tcttctggtt tgctggtgta aacaattctt ttattcaaaa attcctccga
     6421 agcaatctcg tcgctcggat cgatgttctt cttcataagc tcaacacgct caggcttaat
     6481 gtcaaaccca atcacatcga tggtttttgc aaactcaagc gcaatcggca atccaacata
     6541 tccaagcccg ataaccgata attttgtttc gtgattaagt aatttttcgt acgttttcat
     6601 atttgttttt tattgattga ttttattgag aatggggtga aattcgccag tcaacggagc
     6661 aattgaggca ttgcggatgt tatacaccat ttcaatcgaa cgacgtgcat cctcaagtcc
     6721 gaaaccattt cctgcaagta tttgtttgta actgtctgta tgcaactctg taaatccctc
     6781 cgaaaactca aactcagagc catttacacg tattgaacga aaggttcttt tttgcccttt
     6841 gatttcttcc ggcaaatcat tttcgtcaat acttaaaaac caccgcacat tggcgttttt
     6901 caaccggaaa aaacctgccg ctttctgatc actgtgcatg tgaacaatcg attgctcaac
     6961 acttccgaaa atccaggtca gcatatcgaa aaagtgaata ccaatattgg ttgcaacacc
     7021 accactcttg ctctcatccc ctttccatga atgatgatac caatgtccgc ggcttgtgat
     7081 gtatgtcaga tcaacatcaa aaaattcatt gctccttttt tctgtttctt ctttcagatg
     7141 ccggatcgat tcgtgaagcc gcaactgcaa aatattattg atccgttttc ctgtttcatt
     7201 ttcgatttct gcaagcgcat caatattcca cggattcagc accaatggct tttcacaaat
     7261 cacatcggcc tgattacgca tggccatacg tatatgtgca tcgtgtaaat aattgggcga
     7321 acaaatgctt acataatcaa tcttactctc gcctgcacga cgcattttgt caaggtggcg
     7381 atcgaagcgc tcgggttcgc tgaaataatc agcatcggga aaataagaat caataacacc
     7441 aattccatca tatggatcaa gcaatgccgt cagtcgatta ccggtatcgc gtatggcctt
     7501 catgtgcctt ggcgcaatgt aaccggctgc cccgatcaaa gcaaaattca ttggattact
     7561 cattatttct ttgttactat accgtttttc aatacatatt gttctccgct ttcgggacat
     7621 tcagcagtac catcttctct gaaaatcaaa cgatggccat actcactcac ccatcccatc
     7681 tgctttccgg gatttccgat gatcagcgca taaggcaaaa catcttttgt aacaacagta
     7741 ccagcgccaa tcaatgcata ttcgccaatg gtgttgccac atacaatagt agcattggcc
     7801 ccaatcgaag cgcccttctt caccagcgtt tgctcatact gatcgcgacg gataatcgca
     7861 cttcgcggat tggaaatatt ggtaaacacc atgctgggtc ccagaaaaac atcatcttcg
     7921 caaatgactc ccgtgtaaat tgacacattg ttctgaacct tcacattatt accaagtata
     7981 acttctggcg aaacaacaac gttctgacca atgttacaac cctttcccag cacgcatcct
     8041 gacataaggt gcgaaaaatg ccatatcttg caaccttcac caatggtgca gttttcatca
     8101 atcacactgg ttggatgtgc aaaaaaatca gccatattat ttgtttaaaa attcaagaac
     8161 cgatccggtg ataaactcca gctgctcttc gtcaagctct gtgtgcatcg gcaacgaaaa
     8221 aacattctgc gaaagccatt ctgtatttgg aaaatcacct tgtttgtaac ggggatcaag
     8281 atatgctttc tgcatgtgca acggtaccgg ataataaatc atgcatggaa taccacgttc
     8341 ctgtaaaaaa gcaatcaatg cattacggtc aatcccttga ccaacaagcg tgtattgatg
     8401 aaagacatga cgcgattttt tataacggaa tggcgttatc aacttcggat gatttgcaaa
     8461 agctttgtca tagtaatcag ctgccgcaat acgtgctttg atatagttat caagatattt
     8521 cagtttgaca tcaagaactg cggcctgaat gctgtcgagc cgactgttga caccgatttc
     8581 atcgtgataa taacggattt tcatgccatg attaacaaca atccgcagtt tttccgccaa
     8641 tgcatcatca ttggtaaaaa cagctccacc gtcgccataa cacccaagat tttttgaagg
     8701 gaaaaaactt gtacatccaa ggtgcccgat ggctccggtt ttttttatac ttccatcatt
     8761 gaaataataa tccgaaccaa tactctggca ggcgtcttca atcacgtaga gattgaactc
     8821 cttggcaaga gccatgattt cttccatagg cgcagactga ccgaatagat gaacaggcac
     8881 aatggctttg gttttcgggg taatggcacg acgaatggca tcgatggaaa tattcatggt
     8941 gtctttctca acatctacca aaacaggcgt caaatgaagc aatgcaataa cttcagcggt
     9001 cgcaataaat gtaaatgatg atgttataac ttcgtcgccg ggctgcagac ccaaagccat
     9061 catgcatacc tgcaacgcat cggtgccatt ggcacaagga attacatgct ttgcgccaag
     9121 atactgctcc agacttttct gaaaacgctg aacagctgga ccgttgataa atgcagtact
     9181 ggaaacaacc tccatgattg agttgtcaat ttcgctcttg attttttcat actgaccctg
     9241 aaggtcaacc attctgatgg gcttcatcct ctatttttct gcaaagctaa taaaaagatg
     9301 ctttgcttta tataagtgta taaaacaaga ttgtaattga atacaaaaat gagcactgtg
     9361 tatatcaaaa cttcaccaca actcccgtac tcgatccgtt cagtatatct ttcaaagtac
     9421 ttttgatttc aacaatttcc ggatcatctg atttcactgc acgcgcattt tttttcacct
     9481 tttttacttc gtgtggcgtt tcaataattg ttttcacatt aatcattgat gatacttcaa
     9541 cccccatgaa atcctgcaat aagggactat cctcctgttt gttgatttgt tttgctatca
     9601 gaggcccgat gttttttttg ctgaatcgat gcttgccaac tttcatgtat ttgggaacaa
     9661 caaatccata tttcattcca gccagctgta gaagcgcctt tttaaacacc ttgtgatttt
     9721 tgaaatgaaa cgaaacctga tataatccct tttttgtatc tgaaatggtt ttaacatcag
     9781 taatgccaac aacatccttc actgcattct ccaccgtttt tgggaaattc agaatatctt
     9841 ctgatggctt aaatgcatcc atgccgcttt gagtcaaggc ccccatatca agcttcaatg
     9901 aataatcacc gcttttgtca ggataaattt ttatacgttc ctccaactca atgcaggaag
     9961 taataaacaa agcagtgagc gccagaaaaa ctacccgcag tttattgtac aatggcattg
    10021 ggatcaaata cttttttaag aatatcatta atacgtgctg ccggatccgt gcggattttc
    10081 ttctcttcgg ctgcaatctg caggaaaagc gcacccagtg ccttctctgt aacatatttc
    10141 gataaatcag tttctattgg tgtaaccaac ggtatcttgt tgtatgtgcc agtgatatct
    10201 gaccagtatt tggtgcaatt cactgcatcc agcgcctttg taatttcggg tttaaacaaa
    10261 gcagtaagtg atgccgttgt tttagactta aaatactccg tggcagcatt atcagcccct
    10321 ttaagaatgt ttatcgcatc ggtgatggtc attgcagtaa tcgcattcag gaatatcgga
    10381 ccagcttttg cgactgcatt ttctgcacca tgattcattg tagcaacaaa attatcaagc
    10441 agggtttcca tacctagctg ctgcaacttt tccttggctt tgatggcttc ctgcggaaat
    10501 ggaatcatga ataaaggatt tttcaaaatt ccatcagctt ttcccagcat cccaatggca
    10561 ttgttggtgc cgacattcag ggcttccttc aacccatttg caacatctcc ttcactaagt
    10621 acaacgggta cattggttgg aagattcagc ttattaagcg tgtcacaagc cgtaaacata
    10681 accaatacag cagcaaatgc aatcatgtat tttttcatgg caaaaaaatt ttcgtaaagt
    10741 tacaaaatct ctaccagatt tataacttta ctgccgctaa cataaaaatt catcatgaat
    10801 ctacccgaag gatcgctgat aagttacttc agcactctcg ttaagcaaca cggaggaatc
    10861 aatctcgcac agggtattcc gggcttcagc cctccacagg aattgctgga tgaactgaaa
    10921 aacatagcac atgaaaatat tcaccagtat ccgccgggga ttggcaattt tacactattg
    10981 gaacaattac tggcttattt ttctgaccag ttcccgctga aaaaagaaaa tatgatcatc
    11041 atgcagggcg caaccgaagc gctatgcctg atttttttat atgtcatgcg tcaaaccaaa
    11101 ggtggattca cgactctttc attcactccg ccatacgaat cctaccacca acttccaaaa
    11161 caattcggac ttccgttcat cgaactcgat cctgatgaaa acaaacaact gcctcttgct
    11221 caaataaaag aaaaaattga atcggataat gttcggctga tctttttggc ttcacccgga
    11281 aatccgcatg gaatcatgtt ttcaaaagaa agcgtaaacg cattgattga attatgtcag
    11341 caaaaagaat gctttctcgt ttttgattcg gtgtacagaa cactgtattt tgaaacaaaa
    11401 ccttacgttc cgctttcaaa actaaacccc tatcttttta tcgttgacgg attttcaaaa
    11461 tggttttcca tcaccggatg gcgtgttgga tttcttatac acgatgaagc tcattgttct
    11521 tcattacgct cattacacga ctataccggt ctctgtgcca attcagtctt acaggaagct
    11581 ctttcacgtt ttattgcaaa aaataatttt ggggaaactt attccggaaa cctgcgaaaa
    11641 atccttgccg caaattacca attcgcttca aaaaaactcg atgccatggg attcaactgc
    11701 aaaaaaccgg atggaggata ttttgtctgg gcagagcttc ccgaaacgtt ttccaatggc
    11761 tttgagtttg cagtaaaact tttcgattat caaaaagtag ccgtcgttcc aggaattcat
    11821 ttcagcgaaa aagccgaaaa aattgtccgg ttcaatatcg cacggccact cccggaacta
    11881 acagagggcc ttgaaaaaat atcatgtttc ctgaaaaaaa attccgaaaa aacttaaaac
    11941 acaagattat ttttccactc ggcgtattga tccgtctttg ggattcagta atacttgatg
    12001 tatagatgaa ggcagctgaa gaacagtccc taactgacat acacttactt tttccatcaa
    12061 aaccacttca ccgtttccat tgcggattat aaaaaaacat ttttccggga cattatatgc
    12121 caatcctgca ttagcagacc tcttagtatt tgaaacagca gcaacaacag cttcagtgcg
    12181 ctcttcaggc ataatctgat aataatacat gttgtcagta aacaaactgt cttcaatttc
    12241 tgcaacgcct tctgtctgat aaaaactgaa tataaaatca ttgtcaaccg gcttttccgg
    12301 atcaatcaca atgcgataat gcgttatgta ctcaactgtt tttccggtaa aaagtgtgag
    12361 gtattcctgt tgcattttgt tgagttcatc aagcattgaa cgaaaagtag tctcatcata
    12421 atccacttcc tgataaccag aaatgagatt catcttgttt tcagaaatca gtcttatata
    12481 atctgctgca tcttttgcct tgtcttcaag cgacttttcg ataatactat gttgcaaaat
    12541 ctgcttttca atcgttatgg tatcaagaat aatcttttca atgatagtat ctgttttcac
    12601 ttgtgtgttt cctgttgcaa aatgctcaaa cccaacattc attgattgtg aagcgcggtc
    12661 attttcaacc aacagacttt tatccgctgc ttcaaagcct gcatctgaag gattattgaa
    12721 agacacaata caaccatctt cattcaccga taataaaaag ggttccttct ctttcaaacg
    12781 ttttgtttcg atataaaaca atttggattt atcgggaact gaaaaagaat tcagtgcaat
    12841 tccaacaatt gaatattccg ttttgttttc agtaatgaca ttacttatgc cggtaaattt
    12901 tgcagaatac tgcgaaaaag gtcctttaag ataatgtgtc tcttttactg ttatatcaac
    12961 aaccaatctg gtctcaggca gataataaaa cacgccttct tttttcagtt tggtataatt
    13021 gtgaaccgga attaccttat acgtattgca tgatgcaaaa gatataacca gcaaaaacaa
    13081 aataactgac cgcatgtgat tttttttcaa aattaatgat tttcgccgaa actttaaatc
    13141 atttaccttt gcaatcaaat ggataaaaaa tcaaccatcc gcataggcaa caacaaatcg
    13201 ccattaatca tcagcgaaaa tcctgtaatg gattttaaaa gatggtggca attacgtatc
    13261 ggaacacata aaacaacacc tgtcatcatc ttggttgatc gaactgttca ctcagcttat
    13321 agcgtgctta tcggtgattt attaaaaaat ctccctgttt ctgtcattga aacacgacag
    13381 atcgatgccg gtgagaactg caaacaatgg gacaatgccg gggtcattct taaggaatgg
    13441 atcaattttc ccgtaaccaa agacactgta atcatctgca tggggggcgg cactgtaact
    13501 gatcttgggg gctttattgc ggccatttac aaacgtggtt tacgcaccgt ttatattccc
    13561 accactctta tggctatgac cgatgcttcg ctgggtggta aaaatgccat caacgcgggc
    13621 tcagacaaaa accaactggg aaccattagc ttcccgcaat ttacccttgt ttcaaccatg
    13681 tggcttgcat cactgcctgc ccgtgaaata cagtcgggtt atgctgaaat cattaaacac
    13741 gcactgctcg atgattacag ttttgtcaaa acaatacttg acatcagcga caccagtgtc
    13801 cctccacctc tgtcattatt agtcagaagc atccgtgtaa aaatgaaaat tgttaccgat
    13861 gacccatatg aaacacacga acgtctctgg ctgaatctgg gccacagttt cggacatgct
    13921 tacgaaagct ttttctacgc taccggaaat cctgtttcac acggacatgc cgttgccatc
    13981 ggacttgctg aagccctgta tttttcaaca atactgcttg gattttcaaa aaaaatatat
    14041 gctgaagtta tcatctggct caggcaacaa ttcatcattg attccctgcc cgaatggaat
    14101 gagatcagag catctgtttt aaaagacaaa aaaaacatca gctccggaat ccggcttgta
    14161 ttgcttaaat cgcccggaaa tccattgctc cgttcaatgg atgaatcgac ctgccagacc
    14221 ctgcacgccg actttctcaa acaactttaa caacaaaaaa agtcataaaa aaagtgagcc
    14281 gaaactcact tttttaatat ctgttatatg tcagaaatat aaaatccggt tgtcttcgag
    14341 ttcggctttg tttttcaacg ccttgaacaa ttcataattg gccttgctct ggaagaactg
    14401 cattttctgg gaaagaataa ctttatgatc ggccggggct gcttcaacta catcaagtac
    14461 agtgaaaaca aacacaccgg tttcgccttt gataaccttc gacaatttac cttttgaaga
    14521 tgcagtgatg tttccaacaa cttcagattc aggaccaaaa ccaggtaatg aatatgttgc
    14581 aaaagttaca tcaaaggtat ctgtgtcaac actgagtttc ttggccaagg catctaccga
    14641 agttacacca tttaaagcag cattcatttt atccataagc atctgcgctt tcttttctct
    14701 tttggccaat ggctcaacaa tggctttaac tgattcaaac gacgcctgac ctttttcaga
    14761 tcttgacttt aaaaccgcaa taacgtattt ggtttcagta tcaagcgaaa atacaggaga
    14821 aacactgttg atttcagtat cttcattata tgcccaacgc acaacttcgc gtccttcagc
    14881 aataccaggc aacgtaaact ctgtagtctt taccttgtca gcaacacgct tggtataacc
    14941 cctgtcaata atatttttct caaaagcttc ctgtgtctta ctttcagcag caaaaacatt
    15001 ggccaggttg taaatcgaat cactggtagt attactgggc tcaaccgtgc gctgaatggt
    15061 ggcaattttc accttattga ccggttttgt tttgtcaatg accttgatta catgataccc
    15121 gaaaatagtt tcaacaacgg tataagaacc aatggctcca tcaacacaag ccttgttgaa
    15181 ctcaggaacc atcatgcctt cctgaaacca gtcgaagtaa ccggcattct gttgtacaga
    15241 tgggtcgttg ctgtgcgcaa tggcggcctg tgaaaagaaa acgctgtcct ttccgcgaac
    15301 agccagcaaa agactatctg ccatttgttt tgcctgttct ttggttctaa ccacagtaga
    15361 atcggcattt tgtgcaccct gataggcaat gagaatatgt gatgccttga gtgaatcagg
    15421 aatcattttg cgatcaagca acttgtgcat gaaataaata ttattttcga gatatggttc
    15481 cacaaatgtt ccaatattgg caccaaacaa tgtatctgct ttcacaggca acacttcacg
    15541 acgcaaatat gaactatccc aggtgaaatc aatatcagca ttggaattga taaaccctac
    15601 aatatctgca tcggcagtct ttgtaaaacg tgaataaatg gtatccactt tttccttcat
    15661 gaatttatga tcggcttccg atggctttac atcgaaaata acaaaatcaa cagcagctac
    15721 gagctcttcc gaaatgaaat catatttgtg ctcttcgtag tattttttca tttcttcttc
    15781 tgtgacagtg atggttgaat cactgattga agcataacgc aaacccgtat aagtggtcac
    15841 gaattttttg tttttctcaa tgttatcacg ctcaataaat gcttttggca aataataacc
    15901 cttgttgaca agtgtcgtat atttattttt tgtcctttct tcgataatca ttttttcata
    15961 catgtgccag ttctgctgtt cctcgggcga acgctcctcg atactttcca catacatagc
    16021 cacctgattg gggtcgaaaa tgcctgtctc aggattggta aagagctgac gaatcaaagg
    16081 atgaacaaat ttaccggtaa ataactcacc cagttctgca tcgctgacag ccaatcccaa
    16141 cttgtcatat tcttcctgca aaatcaggtt ttccaccatt ttgtcaaata cttcattgct
    16201 gatctggaaa ttatcagatg ctgtaaactg gatttcctgc ccatactgca tcatatactg
    16261 agctttacgc tcttcaacca tgaccgaaaa atcctggtaa ttgacttttt cgccaaaaac
    16321 ctcagccagt ggttcagccg aacgccgtcc gccacttgat gtcataaagt cgcttaaaac
    16381 aaagagcgcc agcgcaactc caatgatccc gatgagtaat cccgaatgtt tccgtatttt
    16441 tccaatggta gccattcttc ttgtatttta ttttaaaaaa cgagtgcaaa ggtaatcatt
    16501 ttgatcaaaa agccctattc tgaggtattt ttatcgtctt ttatttcaat ttccccctta
    16561 accctcatta ttctgtaatt atcaaaccgc tcatccgatt caaatccatc gccccagata
    16621 atcttatccg gcgtgattat cttaataaac tcattggaat aaatccgatt tgcacgctca
    16681 tcccaaatca gatggtcggt gtacattttt tcagaatctg cggtaataat cacaacatca
    16741 tatttcacat caatcctctg caactcatcc aatcgctcac cataacgggc tgtgagaact
    16801 gtttcttcct gtcccagtga atcataaaaa atcactttca atccttccgt caacttcaaa
    16861 acacctttct gatccttgtt gtcaaaacgc ttcatcaccg gcgccgtcaa acacgcttcg
    16921 atatgtccgc tgtcgctgta atacatggtt acatccatgg cctgctcaac cggaagtgta
    16981 tctttaaccg tcacttcctt aatgctgttc atggaattgc cacatgaaaa aagcattgtg
    17041 accgccatag cggccacaat gctgattata aatattttat gcagaccggt caccggacta
    17101 tttacttctg actatggtcg tttcggtgta ccagcacgaa acagtatagc tggatccaac
    17161 tgtaatgttt tcgaaaaaga gttcctccga attcggaaat ccaccataac aggcattgta
    17221 tcttgcattg gcaacactga tgacgttttc atcggttgac actgacttgg ccttcgcata
    17281 tttgtcggca gctgcccaat atccggcttt tttgcttacc ggggtgtctc caccgcaact
    17341 cgaaaccgtt gaataataca aatctccgat gataagatat ggtcttccgt catttggatt
    17401 cagactggcc gactgcagag ctgcattgcg cgcattggca tactggttca tgtttcggta
    17461 tgcatcggca agattcagat aagcttctgc tttttgtatc ggatcttcaa gcccggcaac
    17521 agcttcgctc agatatttgg cggcactact gaagtttttg attttcagaa atgattttcc
    17581 aagtgccaat gccgacattg gggttggtga ctgcttgtac agctcctcgg cagcattgaa
    17641 atacaacgga tcttcggtac agtttttctt ctccatcaac tcggtaagct taagcaacca
    17701 attgagatcg gacttttttg tttcaaattt tgcgctgttg atggcaataa tctgttcgca
    17761 atttgcccat ggatcaaaca aactattaat gttgttctca gcaatttcgt atatctcaat
    17821 tttcttgttg atttttaaag aatctccggg tttctccttc aattcttttc tgtactcact
    17881 caactgaaac tcagcagccg acatggcaat gtcataattt tcataaatca tgctggtgtc
    17941 aagcaagccg gcacgaacca tttcgtcggt aaccttgtaa aatatggaca aaacagcagc
    18001 ttcgctttca tttccctgaa ttttaagcga tttttccagg gtgtaataaa tcagttctat
    18061 ttcacccgga cggaaagtgt acagatcata acctttacgg ccaagaatat acccctcacc
    18121 atataatctt gcacagccaa atgcctcaat attctggtca taaaccatca tcagggtgtc
    18181 aatgtagcgt tctttcgatg cactatcagc agctgtttca atcatataat caacaattcg
    18241 cacaccgtcg agatacaagt tctgactgat caacggagca ttgttgaaac aatatctcca
    18301 gggagcaacg gtatatttta tggcatcacc tttatatctt gaatctttcc attgtttata
    18361 attctcgcgg taaagcgaaa aattctttac gccgttaaca ctgtcagtac catatttcgg
    18421 gcctttgatg ctgcaggatt ttggaaaagt gatactgtcc tgtgcttttg caacatgaat
    18481 tgctgaaaac aacagggcaa aaacgaaaat gaaatttaaa acggttttca tggctctata
    18541 attctaattg tacttcttct tttcaaacca acgctctttg agggaaattc ctaaaacaaa
    18601 ccttatatat ttctcctcca tcagattttg actttgagtt cccattttcc caaattcaaa
    18661 gccagttgta ataactgagc gctgcaatct gggataaatg gacttcaacg gccaggaaca
    18721 gccaaaactt atgccaattt tcgatagttc ttccccttta agagaaaggt aagtctgatc
    18781 ataatgaaaa ccaccatacc aggttactct tttcatatat tttgaaacgg ccgaattatt
    18841 gggagaatat gaaaatccgg cattcagcga caaactgttt ttcaaagaat ccgattctcc
    18901 gaacgaagta taaccagacc agtcctgaac cttaaagtca actccaaacg accagttctg
    18961 atctttggca atcataaaac caccggaaat gctcatagga agacttattg tacctgcaat
    19021 attatctgaa accccggtag tatcaataat atactcactg gtaccacttg cataatagcg
    19081 catagccagc aaactttcct tggcattcag attgaccgga agaccgacgg cagcacccag
    19141 aacgagacgt gttttacgtg aggtgaggga gtcgcggttc agaacgattt catattgaag
    19201 acctgcatct aaagccacat cgcccacata gcgggatgac actgtttttg tgttgtaata
    19261 agcagtagta tcaaaaacta ttcttcttgt ctgcgacaat gaaccaaaca catatgaaac
    19321 attggcgccg atgcttaaat gattggcaac ctcaatagca tgtcccatcc agaatttatt
    19381 aaaacctccg ctgccctcat atttggccgt ataatttgcc aaggaatcaa cagggaaagc
    19441 agcttcaaga ttgtatccaa ccgaactata tggctgaagg ccaaaagcca taccccagtt
    19501 attggccgct ttaaatgcaa aagaaaagct gttgagtgca cctgaaaaac ggttagcagt
    19561 ctgtgatgcc gttgtctgat tggaaaaatt gcctttaaac ccaccattga acaacatggg
    19621 aaacgaatcc tgatcatgca gcaaaatggt gtatgaagcc gggttaaaag aatttacagc
    19681 ataacggtcg cgaaaagccg ctgtcaatcc tcccatcgaa aaagaaacag gttctgtgga
    19741 agtttgcatt tcacccagtc cataacgcga atatggcgac aaaacaaagg actgcgcctt
    19801 taattgaagt gatgaaacca gtaagatcag tccgataaag taaattttca tggattcagg
    19861 ttcagcaaat gattaagccc cacaagaaca atttttggga ctgcaaagat gtgattttca
    19921 tgcgaaatat caaaatatga ggcatctcca ccagctataa aaacagtgcc cccggaagtt
    19981 ctataaagaa attcgtcgat acgataatta atttcagcta aacaaccatt cattacgccg
    20041 ctaataattg aatctttggt tgacattccc atgagttcaa atttattctc tggttcaact
    20101 aacggcagtc tggatgtaaa attgtgcaaa gcccttaacc gtaaactgat tcccggactg
    20161 atggcgccac ccacataata cccgcgctca tcaacataat caaaagtaat ggcggtaccg
    20221 gcctgaataa ccataaaagg gacagcagga ttggataaaa atgccgcacc acaaaccgca
    20281 gcccatcggt cctcgcccaa agtttcaggt gttttgtatt ctgatttcac cggcacctgc
    20341 atgcaacttt tcacattgat aatttcttgg atgcgaaaaa aatcctgttg ctcctctgtc
    20401 agctccacaa cagaagcata aatcttccga gacccgggat attttttcaa aacagcatga
    20461 atgtaatcaa ctgaaaaaaa atctgcaccc gattcgataa ttttttcatc cgaaaaaaca
    20521 gcgtatttgc agcgggtatt acctatatct atggtcagat tcaaattcac agtgtaaaga
    20581 tacatcaaaa aaattttgtc agataaaaat atcgttatat atttgcactc ccaaaaagga
    20641 ggcgtcgtag ctcagtaggt agagcagcgg actgaaaatc cgtgtgtcac tggttcaatt
    20701 ccagtcgatg ccacaaaaaa agccggaata atccggcttt ttttattcat tcaaatgatc
    20761 accaaacacc aatgtagcga tagaattcat cagcattaga gctaaaagta aatgcatcat
    20821 tgatcataaa aatattgcct ttgtcaacca caaacgtgta agctgttttg gccagtttca
    20881 gccggtttga atcgaaggta agcatcagca tcagttcacg gagctgcgca gaagtaactc
    20941 cactaaccga caccgccgta atggccatac gtacgcgggt ttcatcaaat gcttcacggc
    21001 ggatcgacga cagcagcatt ccaaatgcat cgggcgacat acccatcggc atatacgcat
    21061 tgctgccata agaatatgtc ccatagtgac cattgctact cccataatga tttgaatggc
    21121 cgtatccatg tccgttgctg tgattatgtc cgtatccatg gctgtagccc gttgcaggga
    21181 tcatgttctc ggagcgaaga attacaaatg caccaaaacg atcgatggtt ccatacattc
    21241 tcttgttggg cataatatca acagaccccc tgaaaatacg atcaaccatg attccattca
    21301 atgtcattga ttcgcgaaac acttccattc catgacggcc atgctcgatg cgcgatacga
    21361 caacagaccc ggcataactg tcgtactgac gtccatcgat aacaacataa aaatcagcat
    21421 tttcgtttat tgtgatggtc aattccgaat aagcttttgc gaacatagcc ggcgtaattg
    21481 ccatcaggac gagtaaaaga gtaaattttt tcatggcttg ttcctcctta aattttgtga
    21541 gcaatttctc ttcttgtttt caaaggttat gccacaaaaa aaggagttga aaaaacaact
    21601 cccttcagaa aaatatttta tcaaattatt ccagaacaaa gacttttcca ttgaaaacgt
    21661 gccataatcc atcaccatat cgcacttcaa ttctatacga atacagaccc ggtgcggtta
    21721 aattcccatc aacttttcca tcccatccat cattgatatc gcttgattca aaaacctgtt
    21781 ttccccatcg gtcatagatg tgcattgaaa aagtgccttc atccaggccg attccaatgc
    21841 cggatggctt gaacacatcg ttgattccat cattattata tggtgaaaat gcattgggga
    21901 tatagaatgc aatgttcagt tcaacatgaa tgaccgtata gcttttgtcg ctgcaaccat
    21961 tttccgagta gaccgtctgc cagacaatat aatcgccggg gccggaaaaa gtgtgaaccg
    22021 gattggcctc agctgaataa ttgttggtgc ttgaaaaatc gctgaagtcc caataatagg
    22081 tggaagccaa cgtgctcaaa tcagtaaatg tcactacagg attatccatc gaaacccagg
    22141 tagtatcagc aataaaattt gaaaccggta actcgtaaac atctacaatg cccagctctg
    22201 atttagagtt ttcacaacca tcaacgctta ttacgtcaag tttcgcatca tatttcccag
    22261 gattggtaaa tgttttcgat ggcatggcca gattcgatgt cgtgccatcg ctgaaagacc
    22321 agttgtagaa agctgcggat ggattgacgt tggctgaaaa ctgcacaaac aaatcttcac
    22381 agccatcggg aggagatgca taaagctgga tatcaggaat tgcattaaca actacagtaa
    22441 catcggcaga acctgtgcaa ccattgatat ctgttcctgt aacagaataa gtagttgaag
    22501 cagttggggc aaccataaaa gcagcaagtg tattagtcat tgaagcccat tcccaggaaa
    22561 cagcaccact ggcactgagc agagttgaat caccactaca aatctgtgct ggtgaggcag
    22621 aaagctgtat tacaggtggc gggttaacag taatgtaaat tgaatcatag gcctcgcagg
    22681 ttcccatgta agttcccgta acggaatagt aaccagaagt agttggagaa acagtaacag
    22741 aaggcgaaac tgacccatca ctccacacat aggcatcgcc acccgcagca gtgagtgttg
    22801 ttgactcacc cagacacaat accgaatccg gggttgtgat agacacatcc tgagtattca
    22861 gataaacatg aacggtatcg cggattatga gttgagtacc atcgcagtat tcataggcag
    22921 cgatggcaat aaaagtgtcc tgctgggcaa ccatgactgt ttcctgcgca ttgtagccca
    22981 ccagtgacga tccgttaaac caggaaatgg tataagcggg agcaccgttc ggcgtaaagc
    23041 gccatgcttc gttggttgca gtccatggac ctgtattgcg ccccggaggg acaaaggccg
    23101 tagttccggc tgcattctga atgccgataa cgcagttacc gctgttccat gtagaacagg
    23161 tcggtttatt cagaatgtga acttcaataa cattggtgcc ttcatacaaa acaacctgca
    23221 gggtggactg aagactgttg caggaaaaat ggcagatatg atcgaaattc atcacaaaag
    23281 accggcaggg atatgctcca acaattccat agcggaatgt cccacaaaca gatggatcaa
    23341 tatcgtggta tgcaccgaaa attgagttct taaaaaggga tgtacttggg agtgagtttg
    23401 aaaagctcca tgaacaatac tgattggctt tggtcaggtc aaaagtaata atgccgtttg
    23461 ctccaacaac gagctgattg tacgactggc caaaaaaaca aaactgaaaa ggcaacgtta
    23521 ccactggtcc gaaataatca tcctgattaa tcggaatagc tgtacctgaa gtaaacggat
    23581 aaggcggagc atatggaata cttgttaccg tataggtatt tgtgtagccg acatgaaaaa
    23641 catcggctgt caaagtcagg ttactgggac cgcaaatggt tgtatcgttg gtcaaaaaat
    23701 gcggcgatgg acctgcctcg agtgaagggc aacccggagc ctgcgccaaa gcgagaatcg
    23761 gtgcaaaaat aagcgccagt aagagtattt tgtgtttcat ggtttttatc agttcgcaaa
    23821 tttagaaaaa ctttctatct ttagaccgta aaatcatcat taagtttacg tatgagacaa
    23881 aaaattgtta cacaaaagca ggaattgctt caaatcatat caggatcaaa aatctgtttt
    23941 gttgccatgg tcgattcagc tggaaaacct tacaacctgc cgtttaactt cggatttgat
    24001 gaggactaca tctacctgca cagtggcagc gacggaaaga aaatgagaat cctggcagcg
    24061 aatccaaatg tctgcattgc gttctcaaac agcgaagagc ttgcatatca aagtgcagat
    24121 gtggcctgca gccattttat gaaatacaga agtgtactgg tctggggagc agtggaattt
    24181 gtagaagacc cagaggaaaa aatggcaatt ctcaacaaat ttatgaaaca ctatacgggg
    24241 cgtgatggtt ttaaatacaa cactccggcc atcagcaatg ttaaggtatt cagggtatca
    24301 acaaaaaaca tgagcgggaa aacttatggt ttctgaattt caaacaaact gaatatacaa
    24361 ttagggtctg ctgaaaaagt ctgaggcgcg ccagatttga ggcgacaaga cgaaga
//
LOCUS       DAOPKE010000038        24087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2218_length_24087_cov_11.679802, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000038 DAOPKE010000000
VERSION     DAOPKE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_24087_cov_11.679802"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKY63_06575"
     CDS             complement(<1..362)
                     /locus_tag="PKY63_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00314.1"
                     /translation="MKIFVMIFLSLLILSCSKDDFSTGIHSNVLFGMGSCMPFIDESD
                     RDYEKYDGRVYLVNKVAADSLGKSGFILLKQGSTSLVIRNGKLSAELPAGTFVVMTEE
                     YFVNEPANTVTITDGQIT"
     gene            complement(377..1303)
                     /locus_tag="PKY63_06580"
     CDS             complement(377..1303)
                     /locus_tag="PKY63_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00315.1"
                     /translation="MLKIFNNRNFILLLSLILGFIMGDYSIHLASFSAWILALVMVFA
                     TTGFSFSDWRPIGKTIRIVGISFLLNFVVFGLLLFGAGLLFFDNELMITGILLLVISP
                     PGPSVIPFTAAMKGNISFAVTGVFGLHLISIIITPIALLLLLGNSTISTQSVLTIITT
                     VIIIPLIISRILRRTPLMSSIEKIRGHVINWGFFLIIMPIIGMSKSMIIAQPTMVLTN
                     SLLFAAVMFGGGLLFNIVSKKIRLDKPTTIAANLIFTTKSSAFAAVASFSLVPPEAGL
                     PLAMHAVFVTLYFIIFDFIINKKQKAPLIISE"
     gene            1349..2047
                     /locus_tag="PKY63_06585"
     CDS             1349..2047
                     /locus_tag="PKY63_06585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPF00316.1"
                     /translation="MELFFHPELINGIFEPGENEVRHLRVLRKKPGDIVWFTDGKGIL
                     AECVVVDAAPKKFLLEVKKIEIISRPKPLLTLAVSPLKNPDRFEWLVEKCTESGVGTI
                     VPIMCERTESSLKKSDRLQRIIQAATIQSLQYYMPELSQPMQFLEFLKAYDAKTKLIA
                     WCDDVVKTPILSALHEGEDTIILIGPEGDFTKEEFDAAMAMGYQAISLGQNRLRTETA
                     AVLAAQAFYLKNSK"
     gene            2111..4996
                     /locus_tag="PKY63_06590"
     CDS             2111..4996
                     /locus_tag="PKY63_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00317.1"
                     /translation="MALSWNEIKARAISFSKEWSDTANEDADAKPFLVDFFNVFGINR
                     KRVSTFEHRVKKLDDSDGYIDLLWKGVILIEMKSRGKNLDKAFNQAKEYLHGLKEYEL
                     PKFVLVSDFDVFRLHDLEENSVSEFHLRDLVSNVHYFGSLIGYSKKIYKEQDPANIKA
                     AELMGKLHDRLKEIGFTGHPLEVYLVRILFCLFSEDTTIFEKRQFQEYIENRTSEDGS
                     DLASHLQELFQVLNTPLEGRYTNLDEQLSEFPYVNGKLFEEILPIASFDTKLRKALLD
                     CCQIDWSRISPAIFGSMFQSVMNPVERRNLGAHYTSEKNILKLIKPLFLDELYSELES
                     CKGNRSKLLQLQKKLGSLKFLDPACGCGNFLVITYRELRLLEFDILRQLYKGQFATSL
                     EGIIELNVDQFYGIEYEEFPARIAEVALWLIDHQMNLRLSEEFGQYFVRLPLKKAACI
                     TIGNALEIDWNSLIKNQPIEIEAENTTITLNEPEAHEYTSVHIQTRNLTIIDERKSES
                     VQLYTSAGFNFILGNPPFIGKSQQNAAQKSDMERIFAGYSGTGVLDYVTAWYLKASRY
                     IQNTRTRVAFVSTNSIVQGEQVGILWNILFNQYGIKIHFAHQSFSWSNEAKGNAAVHV
                     VIIGFANYDIISKRIFTYDDIKGEYHEISTNNINPYLVEGKDITILSRSRVLCGGIPE
                     MSWGNKPVDGGNLILSNEEKNEIVNTEPHINNFIKPLICAKEFLNSEKRWCLWLVGVD
                     PSEIRKSNFLLDRVKRVKQVRESSKDAGARRLAQQPGQFRDIKTYEKYILIPAHSSEN
                     RKYIPIGFFGKNDIAHNSCLVVPNASLFHFGMLSSEIHMAWVRYVCGRLESRFRYSKD
                     IVYNNFPWPENPTETQIARIEKLAQDVLDVRTQYPKSSLAVLYDPITMPPALVKAHNE
                     LDKAVDLAYRPQPFVSDAKRMEYLFELYEKYTADLFTTKKKEKKKSK"
     gene            4993..5643
                     /locus_tag="PKY63_06595"
     CDS             4993..5643
                     /locus_tag="PKY63_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPF00318.1"
                     /translation="MKLFFAIILLSVVTISTSAQVKIALLKYSGGGDWYANPTSLPNL
                     VKFCNANMGTNIVTDIPTVDPGSSDIFNYPFIHMTGHGNVMFSEQEVENLRQYMLSGG
                     FLHIDDNYGMDVFIRPQIKKIFPEYELIEIPFSHAIYHQQYDFPNGLPKIHEHDAKPP
                     KGYGIVVDGRIVLFYTYECDLGDGWESHDVHNDSQETRTKALQMGANIIRYVFNGD"
     assembly_gap    5763..5862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6005..6442)
                     /locus_tag="PKY63_06600"
     CDS             complement(6005..6442)
                     /locus_tag="PKY63_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPF00319.1"
                     /translation="MKPILTLFFTILLCTIIQAQNTNTAPDDSIYTVVEQAPLYPGGV
                     EALMKYLAENIQYPDLARQENIQGTVYVSFVVEKNGEVSNVKILRGIGGGCDEEAMRV
                     VRNMSDWTPGYQNGEAVRVQFNLPLRFILTGPSFKLFKKKNKK"
     gene            complement(6457..6879)
                     /locus_tag="PKY63_06605"
     CDS             complement(6457..6879)
                     /locus_tag="PKY63_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPF00320.1"
                     /translation="MRFLSPLILSLLLLSAMTNIAQNEGSFYTDTLTGTHYDYYKIVE
                     VAPDFPGGTVALMKFLRKNTRYPKDAKKNNIKGTVYTSFVIDETGKVSNARILRGLGY
                     GCDAEALRIIGLMPKWIPAQTKGKPIKCEFNLPIRFGK"
     gene            complement(6904..7896)
                     /locus_tag="PKY63_06610"
     CDS             complement(6904..7896)
                     /locus_tag="PKY63_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPF00321.1"
                     /translation="MLKRICLILLTIFFAATLPAENNIEPNFFKVSNFEKVSQVEKVE
                     KNSNLFSPGDTTKPLFKKWQINTFSGVFYAGSLTGLSFLWYSDYSTGHFHFFNDMDEW
                     KGMDKLGHMTSAFHLTRVQSDLYRNAGYTRGQSARISTICSFSYMTVIEVLDGFSDGW
                     GFSMGDMCANTLGVSLFALNEMTGKENLLSVKMGFHQTSYAQYNPSLLGENLLQQPLK
                     DYNGQTYWLSMNIKECLKKESRFPAWLNVAVGYGADGMIGGDYNPAEVDGNAVPFFNR
                     YSQWYLAPDIDFSKIPVRGRGWKLLMRSLNFLKCPAPALEWNSENGLKVHFLYF"
     gene            complement(7911..8726)
                     /locus_tag="PKY63_06615"
     CDS             complement(7911..8726)
                     /locus_tag="PKY63_06615"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF00322.1"
                     /translation="MRSLLFIALLFSAMWASALDPSLTYAVTPADYGLNYEEVSIKTS
                     DNLNLKGWLFKPSEVSYKMIILSDNGEGNMADLIEVATNFVSLGYNVLTYDYRGFGQS
                     DAFNINPEFYIYTQFALDLNAAIDYVSKYHSKCRTVCLYGQGVGAALSLAIGATRCSE
                     ISKIIADSPYSTLDDVQKAIKTTTGKEVKLPLGFDKNQLEPKFALEAKGASLNGILII
                     YGKIDPVYNSDMVKKISGIRGGITKTYIVKEADHTSTFSTDKEGYFKEIKAFL"
     gene            complement(8773..9300)
                     /locus_tag="PKY63_06620"
     CDS             complement(8773..9300)
                     /locus_tag="PKY63_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00323.1"
                     /translation="MELALDLAKIVLPALMVFLTAFFVLKSYLENEQKKKELDARFEQ
                     KRVSLPIRLQAYERLALFLERINPESIVLRMNQVGMSSKELQALLLQTIRMEFEHNLS
                     QQVYISNQAWDMVKNAKEDVIRLINTAGATMQPNASAIDLSTSIFEESLKTKEGLLQK
                     ALIFLKNEGRQYLDA"
     gene            complement(9748..10743)
                     /locus_tag="PKY63_06625"
     CDS             complement(9748..10743)
                     /locus_tag="PKY63_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPF00324.1"
                     /translation="MKKLLFPVLVMLIVSCGNKPQTSLVEWSGNAQGTTFHIKWYGSP
                     EQVKKESVDSILHAFDLMASLYNDSSLICRINRGENPVLSAELITQLNKSLEISEKTD
                     GYFDITVGPLVRAWGFGRQKSTDPDSTTIDSLKQLVGYQKISISRNHLVFDSAGIQID
                     LNAIAQGYSVDLIAEFLESKGVHDYMVEIGGEVRVSGKKPDGASWVVGIERPAENEFA
                     EQEIFRKLAITDISIATSGSSRKFYVKDGVKYSHTIDPHTGYPVSHSLLSVTVITKKC
                     MDADAYATAFMVMGINKAKIILEQNKEMEAFFIYSAPDGTLKTDSTSGFGKYFVE"
     gene            10824..11480
                     /locus_tag="PKY63_06630"
     CDS             10824..11480
                     /locus_tag="PKY63_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00325.1"
                     /translation="MKKILTLFGSVVFAAMFVPMFFSSCEPEEDTEVPEPTSTFFSAV
                     IGDNNKSITPGVNGYLVEPYDTGYSDASGTHYISGIRLYQSGSGYYVSSREEFKIELV
                     NLFDSAATNRDSLFHAYLSQATLPWYNPTQVADSSKHTGMRILWRTAEGDWYATDEAA
                     QSGTMTVDTTTDVTVPGGISTHEVYLQYNCTLYEKNGSGSLVLTEGRGRFSFINSMFY
                     "
     gene            complement(11521..12264)
                     /locus_tag="PKY63_06635"
     CDS             complement(11521..12264)
                     /locus_tag="PKY63_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPF00326.1"
                     /translation="MLLKKYFVAIIISFSILPLNAQLHPVCDSASLIQHQYFSLCYNE
                     KTEQADWVFHFITPERLKNKVASRSDDFRPDKKIKTGSAELKDYKGSGYDRGHLCPAG
                     DMGFDSTAMSESFLMSNMSPQEPGFNRGIWNSLEQRIRNWGLLFDTLFIITGPIFYDT
                     AYSFIGENKVAIPDACYKVLLGKKTDKFYTIGFIIPNSEGLKNYLSYCETIDRVEELT
                     GLDFFSSLNDSTEKQIEESYSCFFINGKD"
     gene            complement(12270..12953)
                     /locus_tag="PKY63_06640"
     CDS             complement(12270..12953)
                     /locus_tag="PKY63_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HPF00327.1"
                     /translation="MNKGKFIVLEGLDGSGKSTQTTLLLDAIRNRGIDAEFIHFPRTD
                     SSSPFYGPMLKRFLKGELGSLAEVDPWFVAMLFAGDRRNEGPAIAALLNEGKWVIADR
                     YVLSNIAFQGAKINSQKEKSELAHWILNTEYNVFHLPVPDQTFFIHMPLSFCRGNVLN
                     QRSGADRDYLDGKADIHEADFSFQNEVYEMYRYMLSKPEFGLTDLQINEPVPDPAEIH
                     SMLMQLIEC"
     gene            12962..13837
                     /gene="folP"
                     /locus_tag="PKY63_06645"
     CDS             12962..13837
                     /gene="folP"
                     /locus_tag="PKY63_06645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPF00328.1"
                     /translation="MLYICEDINSMESSAKKILLNIRGSLIEFQTPAVMGIINITSDS
                     YYSASRTEDEKRAYERCGTLLDEGAAVIDVGAVSTRPGSDYPGVEEELRRLASVFPML
                     RKEFPDAIFSIDTSNAVVARQMIDLGADIINDISAGAHDSDMFALIAEKKCPYIMMHM
                     RGWPKDMQKCTKYADVVKSINDYFAQKIEKLQQMGVSDVFLDPGFGFSKTLEQNFLIL
                     KNLQTFTFHDKPLVVGISRKSMVYKALKSTPEDVLPATIALQTIALLKGASILRVHDV
                     AAAVQCVQVLGLMNE"
     gene            complement(13996..14868)
                     /locus_tag="PKY63_06650"
     CDS             complement(13996..14868)
                     /locus_tag="PKY63_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF00329.1"
                     /translation="MESKYDNILIPVDFGRRTDIQIQQAYYLARLMNSSMTLLHVINQ
                     RNSISYQDQQSSVSYVGADEDIIVKIRRLATHFSQKFSHDVDYAVLEGNVYEQIVDFA
                     AKTHARLIVMGKSGKTSPLQNSIGKYTAQVVRMANCPVLTINSIVKTSFDKILLPLDL
                     SKQIRNKIAVAVLFSRYYGSTIKLLTVITRDNFSKEKEFTEKMNKTREFLTENNVACT
                     TEIIYNFSANTSIADRIIQYSSDTESDIVMIMTQQEQDWRHTFVGSTASNIIKNSQIP
                     VISITPNDDAVRNI"
     gene            14913..15779
                     /locus_tag="PKY63_06655"
     CDS             14913..15779
                     /locus_tag="PKY63_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPF00330.1"
                     /translation="MKKIAESELILREDGAVYHLNLKPGEIADNIILVGDPGRVELIS
                     SLFDKVTVSKQNREICTRTGTYKGKKISVISTGMGPDNIDIVINELDALVNIDLKKRM
                     PKKERKSLNLIRIGTSGGIQKSIPVGSFLFSSHGIGTDGLMHFYAGSIKKRCPEIELA
                     LANQVGWPGTWAAPYVVEGSAKLIKKLSTGHPTGMTMTFGGFYGPQGRELRLGLHYPE
                     FNDLVAAFDHKGLKVTNFEMETSALYGLAKMLGHNACTVCVVIANRADKTFIKDYHPP
                     MKELIKLVLDRI"
     gene            15909..16181
                     /locus_tag="PKY63_06660"
     CDS             15909..16181
                     /locus_tag="PKY63_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPF00331.1"
                     /translation="MSKQQNVLPEKKQTFVFGRMNYILMLIGLGVMFLGYILMMGGGS
                     EDPNVFSEELFNTQRTTISPILIIAGFVIEIFAIMHRPKAKDNSEE"
     gene            16178..16960
                     /locus_tag="PKY63_06665"
     CDS             16178..16960
                     /locus_tag="PKY63_06665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPF00332.1"
                     /translation="MSWFDAIIIAIVEGLTEFLPVSSTGHMIITEALLGHKPDDFSKA
                     FIVNIQFGAILAVVVMYIRRFFQSFDFYLKLAVACIPLVIAFLLEAFIDQMLESVLVV
                     AIALFAGGIVLVFVDRWFKNPEKAEKVLSSKNAFVIGLFQIIAVIPGVSRSAATIIGG
                     LSQGLSRKQAAEFSFFLAVPTMLAASAYKLYKNHEILGSVENLQLLAIGNLVAFVVAI
                     LAIKGFIAFLNRYGFRFFGYYRIILGGVLIALILAGYNLSVV"
     gene            16961..17662
                     /gene="truB"
                     /locus_tag="PKY63_06670"
     CDS             16961..17662
                     /gene="truB"
                     /locus_tag="PKY63_06670"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPF00333.1"
                     /translation="MFDFASGEILLFDKPYTWTSFDVVHLIKNCLKKSMPEPVKVGHA
                     GTLDPLATGMLVVLTGKKTREMEIIQAQEKEYTGTFTIGATTPSYDREREVDQTFSID
                     HITEEMIVEAAKTFVGEIMQLPPDHSAVKVDGKRAYEYARKGKSVDIKPRPITISEFE
                     ITRVALPEIDFRVTCSKGTYIRSLARDFGEALNSGAYLSSLCRTRIGEYRIEDAMHPK
                     EFTALIEKMKQDGML"
     gene            complement(17776..20334)
                     /locus_tag="PKY63_06675"
     CDS             complement(17776..20334)
                     /locus_tag="PKY63_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00334.1"
                     /translation="MKILVIAVSFIFFSLSAIGQTIVNLDAATHNTTQNGCSYWFYDD
                     GTASGDYTNNQDRWITFASSDVLNTHIKASFAAFDIEPGDTLIIYDGPNTTSPVIGKY
                     NNNNPLTSPNTMVQASIANVSGDLTFRFKSNGSTVSDGWNCSIGCIPQCQEILAAVDT
                     LQMLPLPNDSNYVDICFGDIVTFAAMGAGPGVFPENDLLYHQDSSTTLFIWDFGDGTI
                     DTGRVIDHLYTSVMGYDVFLTVIDTRGCTNTQLLALRVRVSGNPIIDIKPLPDICAGD
                     TIDLVVGENATSTIVVAPTGSFQIATELYDSTTFIPDGPYCSTNCYYTPVTFSTFPPG
                     SSITSASDIASICINMEHTFAGDLAFSIICPNGTTVQMDSYDNSGGSDLGISNSTTYD
                     DCDPAQNPPGTGWTYCWSEVYPQQGTLNTLDAGTSPIPATDTINNTGYITTETPFSAL
                     VGCPLNGTWSIEICDEWGQDNGYVFWWSLTLLNQTQSGAWSYTVPIDSVSWAGPSITS
                     TSDSTAFIAPQSPGIFTYTATVWDAFGCSYDTTLNVEVLANPQPDLGADTTVCEGSVL
                     TLDAGSGNQYFWSNGAITQTINPDLSDNYAVTVTNSNGNIACYGSDTINITMIPWASV
                     NLGPDLCETESITLDAQNPGFDFLWSNGATTQTTTISSTGVYSVTVSYGGSSLCSDVD
                     SINVNVIPSPIVNLGPDQEICRHEFLDFDVSQSTPQYTYHWSTGSTSPYLHVEGLPVG
                     PAVYSVTVTGCDSKSDTVVVSVIACDLNIPNIITPNGDGINDVFEVFNLPFYPNSILM
                     VYNRWGKKIYENSNYMNDWDGENFADGTYFIILRVNYGNEEYEDHHGTLTIMRE"
     gene            complement(20346..20762)
                     /locus_tag="PKY63_06680"
     CDS             complement(20346..20762)
                     /locus_tag="PKY63_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00335.1"
                     /translation="MNKIILLIACFLLVTPAIAQNSVQADARLIEAFGNSKVQTLSNQ
                     YPDSIRYYNFFLDNAFEIWKNDVVAEYINPFETRSIILSKDNLAALNNLKAFNILTTG
                     LQWDKNETLWVKIENADFYIKLHSLSYIENKFKAGK"
     gene            complement(20871..21836)
                     /locus_tag="PKY63_06685"
     CDS             complement(20871..21836)
                     /locus_tag="PKY63_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPF00336.1"
                     /translation="MKIIGIGNALTDLMTQIESDDILRNLNLPKGSMQLVDATMSQRA
                     SDATKHFKRTLASGGSAANTIHGLARLGMKTGFIGKVGKDDLGDFFEKDMVKSGIQTH
                     LIQSATPSGLALALVSPDGERTFATYLGAACELQDIDIDADVLKNYDILHIEGYLLQN
                     YKLISHSIKTAKELGLKVSLDLASYNVVEEHRDFLNEMIDLYTDIIFANEEESKALTG
                     LEPEESAKYLSQRVDIAAVKTGPGGSFVAMNGEITAVPTQKIIPVDTTGAGDLYAAGF
                     LYALSKGKSAYECGRTGSVIASEVITTIGAKIPEEKWSTLVQLTK"
     gene            21927..22000
                     /locus_tag="PKY63_06690"
     tRNA            21927..22000
                     /locus_tag="PKY63_06690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21961..21963,aa:Asn,seq:gtt)
     gene            22152..22370
                     /locus_tag="PKY63_06695"
     CDS             22152..22370
                     /locus_tag="PKY63_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPF00337.1"
                     /translation="MFDFKKLIVYQKARDFALTTTELLANNKFDKAVNDQLRRASLSI
                     MLNIAEGSSRFSNKDRKYFMVVARENDL"
     gene            complement(22486..22881)
                     /locus_tag="PKY63_06700"
     CDS             complement(22486..22881)
                     /locus_tag="PKY63_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPF00338.1"
                     /translation="MNKILRKIFWKEEGMEFNDNPKDISGKFTLKYGNMTIGFLKYEK
                     SVWYFQYSDEFRTQELIKPIIDFPDSSKTYESDSLWPFFASRIPSINQPYQLKKIQKA
                     SVNKEDSVALLRIFGKTTINNPFQLSVNG"
     gene            complement(22874..23893)
                     /locus_tag="PKY63_06705"
     CDS             complement(22874..23893)
                     /locus_tag="PKY63_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPF00339.1"
                     /translation="MRKRKTIHPLKIIDYTGDNPKPGAIPFVKKHHYFIIKNTSIDGD
                     APKDFISRYEYGRGRKNNPDSWPRYIAKHGHKHYPTEAITEHLLNRIGEVLGLNMAKS
                     CLARLGGQIRFLSEYFLSNRCTQVLEHGADLYAGYLNDKDFVSEVENQNRSPEFFTVQ
                     FTQQTLRHFFPDHFHQLMSSFYLLLLFDAITGNNDRHYYNWGIVRDIRNSNPEFSPIY
                     DSARGLYWNTSEKDLQDKYGSPHLRMPAIKKYAEHSYPKIGWDNIGKINHFDLIKHSM
                     SQPDFPYKSAADILNNTDNTLAITNLINNEFAPLMSDFRRKLIEQTLLYRIECLKNVL
                     SLHHE"
BASE COUNT         6783 a         4691 c         5362 g         7151 t
ORIGIN      
        1 tcagtaatct gcccatctgt gatggtgacg gtattagctg gttcatttac aaaatattct
       61 tcagtcataa caacaaaagt gcctgctggt agttctgctg atagtttgcc attgcggata
      121 acaagactgg tgcttccttg cttcaacaaa ataaatcctg atttgcccaa actgtcggct
      181 gctaccttgt ttacaagata aacgcgtcca tcatattttt catagtcacg gtccgattcg
      241 tcaataaacg gcatgcaact tcccattccg aagagtacgt ttgaatgaat tccggtcgaa
      301 aaatcatctt tggaacacga caggattagc aaagacagaa atatcatcac aaatattttc
      361 attttattca ttgcatttat tccgatatga ttaacggagc tttttgtttc ttattgatga
      421 tgaaatcaaa aatgatgaaa tacaaagtta caaaaaccgc gtgcatggcc aggggtagtc
      481 cggcctcggg aggcacaagc gagaatgatg caactgcggc gaatgccgaa ctttttgttg
      541 taaatataag gttggcggca atcgtggttg gtttatcgag ccggattttt tttgacacaa
      601 tattaaatag taacccgccg ccaaacatta cagctgcaaa aagcagcgag tttgtcaaaa
      661 ccatcgtagg ttgtgctata atcatcgatt tgctcatgcc gataatgggc atgataatga
      721 gaaaaaagcc ccaattgata acatgtcccc tgattttttc aatggaagac atgagtggag
      781 ttcttcgtag aattcggctg atgatgagtg gaattataat aactgttgtg atgattgtca
      841 atacagactg agtgctgatg gtggaattac ccagtagtag cagtaaagca ataggagtga
      901 tgatgattga aatcagatga agtccgaaaa ccccggtgac tgcaaaagaa atgtttcctt
      961 tcatggccgc agtaaaagga atgacagaag gtcccggcgg gctgatgacc agcagcagaa
     1021 ttccggtaat cataagctca ttgtcgaaaa acagcaatcc tgcgccaaac agcaaaagtc
     1081 cgaaaacaac gaaattgagc agaaaggaaa ttccgacaat gcgtatggtt tttccaattg
     1141 ggcgccagtc actgaagctg aatccggtgg tggcaaaaac catcaccaga gccagaatcc
     1201 atgcggaaaa agaggccagg tgaatgctgt agtcgcccat gataaaacca agtatcagag
     1261 acaggagcag aataaaattt cggttattga atattttaag catattgttt gatgcgattg
     1321 tgtaactttg gaatgcaaaa ttacttcaat ggaattgttt tttcatccgg aactgataaa
     1381 tggcatattt gagccaggcg aaaacgaagt gcggcatctg cgtgttcttc gaaaaaagcc
     1441 gggcgatatc gtgtggttta ctgatggtaa gggaatcctc gctgaatgtg ttgttgtaga
     1501 tgccgccccg aaaaagtttt tgcttgaagt aaaaaaaatt gaaataataa gccgtccgaa
     1561 acctctgtta acattggctg tgtcgccttt gaaaaatccc gatcggtttg aatggctggt
     1621 ggaaaagtgc accgagagcg gtgttggaac aattgttccg attatgtgcg aacgtacaga
     1681 atcatcgctg aaaaaatccg atcgcctgca gcggattatt caggcggcaa ccattcagtc
     1741 gcttcaatat tacatgcccg aactttcgca gcctatgcag tttttggagt ttctgaaagc
     1801 gtacgatgca aaaacaaagc tcattgcctg gtgcgacgat gtggtcaaaa cgcccatttt
     1861 gtctgcttta cacgaaggcg aggacactat cattctcatt ggccccgaag gcgattttac
     1921 caaagaagaa tttgatgcag ccatggcaat gggttatcag gcaatatcgc tcgggcaaaa
     1981 ccgtttaaga accgaaaccg ctgccgtgct tgctgcacag gcattttatc tgaagaattc
     2041 gaagtgattg ttgaaaagta aattcaataa aaccaaactg tttaattaat ttagcaatag
     2101 aaatatttac atggcactta gctggaacga aataaaagca cgggcaatca gtttctcaaa
     2161 agaatggtct gacactgcaa acgaagatgc agatgcaaag ccgtttttgg ttgatttttt
     2221 taatgttttc ggtataaaca gaaagcgtgt ttcaacattt gaacatcggg taaaaaaatt
     2281 ggatgactct gatggataca tcgatcttct ttggaaaggc gttattctga ttgaaatgaa
     2341 gagtcgtggc aaaaatctcg acaaagcttt taatcaggcc aaggaatatt tacatggttt
     2401 gaaagagtat gaattaccga aatttgtttt ggtgtccgat tttgacgttt ttcgtctgca
     2461 cgatcttgaa gagaacagtg tttctgagtt tcatctgaga gatcttgtaa gcaatgtgca
     2521 ttattttggt tctttgatcg gatattccaa aaaaatctat aaagagcagg atccagccaa
     2581 catcaaagcg gctgagttga tgggcaagct tcatgaccgg ctcaaggaaa ttggttttac
     2641 tggccatcca cttgaagtgt atctggttag aattttattt tgtttgtttt cagaagacac
     2701 cactattttt gaaaagcgac aatttcagga atatattgag aatcgaacaa gcgaagatgg
     2761 gagcgatctg gcttcgcatc tgcaggaatt atttcaggta ttgaatacac cgcttgaagg
     2821 acgatacaca aatctcgatg agcaactctc ggaatttccg tatgtgaacg gaaagctatt
     2881 tgaagagata ttgcccattg ccagttttga cacaaaattg cgaaaggcac ttctcgattg
     2941 ttgccagata gactggagcc gtatttcgcc agccattttt ggatctatgt ttcagagtgt
     3001 catgaatccg gttgaacgtc ggaatcttgg agcacattac accagcgaaa aaaatattct
     3061 gaagcttatc aaaccattat ttctggatga attatatagc gagcttgaat cctgtaaagg
     3121 aaatcgaagc aaactattgc aattacaaaa gaagcttggg tctctaaaat tccttgatcc
     3181 agcctgcggt tgtggaaatt ttctggtcat aacgtatcgc gaattgcgac tgttggaatt
     3241 tgatattttg cgtcaattgt ataaaggaca atttgccact tcgctcgagg gaattattga
     3301 gcttaatgtt gatcagtttt acggaattga atacgaggaa tttcctgcac gaattgccga
     3361 agtggcattg tggcttattg atcatcagat gaacttgcga ctatcagaag aattcggtca
     3421 atactttgtg cggcttccat tgaagaaagc agcttgtatc acgatcggaa acgcgttgga
     3481 aattgattgg aattcattga ttaaaaatca gccaattgaa attgaagctg aaaatacgac
     3541 catcacatta aatgagccag aagctcatga atacacttcg gttcatattc agacgcgcaa
     3601 tctgaccatc atcgacgagc gaaaaagcga gtccgtccaa ctttatacat ctgctggttt
     3661 taattttata ctcggaaatc caccatttat cggtaaatct cagcaaaatg ctgcacaaaa
     3721 gtccgatatg gagagaattt tcgcagggta ttcgggtacg ggggttctcg attatgttac
     3781 ggcatggtac ttaaaagcat ccaggtatat tcaaaataca agaacaagag tagcttttgt
     3841 ttcaacaaat tccattgtcc aaggtgaaca agtagggatt ttatggaata ttctatttaa
     3901 tcaatatgga attaaaatcc attttgcaca tcaatctttt agttggagca atgaagcaaa
     3961 aggaaatgct gcggttcatg ttgtaatcat tggatttgca aattatgata taatttcaaa
     4021 acgaattttt acttatgacg atattaaagg tgaatatcat gaaatttcca caaataatat
     4081 aaatccatat ttggtggagg gtaaagatat aacgatttta tcaagaagta gagtactatg
     4141 tggtggtatt cctgaaatgt cctggggaaa taaacctgtt gatggtggaa atttaatttt
     4201 aagtaatgaa gaaaaaaatg aaatagttaa taccgaaccc catattaata atttcattaa
     4261 acctttaata tgcgctaaag aattcttaaa tagtgagaaa agatggtgtt tatggcttgt
     4321 tggtgtggac ccttcagaga ttcgaaaatc aaatttttta ttagatcgag ttaaaagagt
     4381 taaacaagtt cgcgaatcaa gtaaagacgc cggggctagg agattggctc aacaaccggg
     4441 tcaatttaga gatataaaaa catatgaaaa atatatttta ataccagctc attcatcaga
     4501 gaatagaaag tatatcccta tcggtttttt cgggaagaat gatattgcac ataatagttg
     4561 tctggttgta ccaaatgcat cattattcca ttttggaatg ctttcttctg aaatacatat
     4621 ggcatgggtg agatatgttt gcggtcgcct tgaaagtcgg ttcagatatt caaaagatat
     4681 cgtctataat aatttcccat ggccggaaaa tcctaccgag acacaaattg cccgtataga
     4741 aaaactggct caggatgttt tggatgttcg cacacaatat ccaaaaagct cgctggctgt
     4801 tttatatgat cctattacaa tgccgcctgc attggtaaaa gcgcacaacg aattggataa
     4861 agctgttgat ctggcataca ggccacagcc ctttgtcagt gatgctaagc gtatggaata
     4921 tttatttgag ctgtatgaaa aatatacagc ggacttgttt accactaaga aaaaagaaaa
     4981 gaaaaagtca aaatgaaatt attttttgcc attatattgc tctctgttgt aacgataagc
     5041 acatctgcac aggtgaaaat cgccttactc aaatacagcg gtggcggcga ctggtatgcc
     5101 aatccgacat cgttgcccaa cctggtgaaa ttctgcaatg caaatatggg aaccaatatc
     5161 gtaacagata ttccaacggt tgatcccgga agcagcgata ttttcaatta tccgtttatc
     5221 catatgacag gccacggcaa cgtgatgttc tcggagcagg aagttgaaaa cttgcgccag
     5281 tacatgcttt caggcggttt tttgcatatt gatgataatt atggaatgga tgtgtttatt
     5341 cgcccacaga tcaaaaagat ttttccggaa tatgaactga tagaaatccc gttcagtcac
     5401 gcaatttatc atcagcaata tgattttccg aacgggttgc cgaaaattca tgagcacgat
     5461 gcaaagccgc cgaagggcta tggtatcgta gtagatggcc gaattgtttt gttttatacc
     5521 tacgaatgcg atttaggcga tggctgggaa agccatgatg tacacaacga ttcgcaggaa
     5581 accagaacca aagcgctgca gatgggtgcc aatattattc gctacgtgtt taatggggat
     5641 tagtgcacaa ggaaatatgc atcagctatc aggtttttgt cttgtgtgaa ttttaatttt
     5701 tcagaatttt ttagcgattg tcaatcatcc aaaatgaacc tgatcggcag attgaattgc
     5761 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgttctcc tcccggaaat
     5881 tcgggctgtt tttccaccag attgaatacc gaaatgtctt cggattctgt atgagttgaa
     5941 tctgttgact gcgcctgcag cacaattgcg cagattgaaa atattataat caataaggcg
     6001 tttttcattt tttgtttttc tttttaaata atttaaaact tggtccagtc agaatgaagc
     6061 gtaaaggcag attgaactgc acccgaacgg cctcaccatt ctgataacct ggtgtccagt
     6121 cagacatgtt tcttacaacc cgcatggctt cttcatcaca gccaccacca attccccgga
     6181 gtatttttac gtttgaaacc tctccatttt tttcaacaac aaatgaaaca taaacggttc
     6241 cctgaatgtt ttcttgccgc gccaggtctg ggtactgaat gttttccgca aggtacttca
     6301 ttaaagcttc tactccgcct ggatataacg gagcttgttc aacaacggtg tagatgctgt
     6361 cgtctggtgc tgtgttggtg ttttgtgcct gaatgattgt gcagagcagg attgtaaaaa
     6421 acagggtcaa gattggtttc attttcaatt catagattat tttccaaaac gaattggcag
     6481 attaaattcg cattttatag gtttgccttt tgtttgagca ggtatccatt tgggcatcaa
     6541 accaattatt ctcagtgctt ctgcatcgca tccgtatccc agtcccctca gtattctggc
     6601 atttgaaact ttccctgttt catcaatgac aaatgaagta tacacagttc ctttaatgtt
     6661 gttctttttt gcatcttttg gatagcgggt gttttttctt agaaacttca ttaatgcaac
     6721 agtcccacca ggaaaatcag gagcaacttc gactatttta taataatcgt aatgcgtgcc
     6781 ggtgagagtg tctgtgtaaa atgaaccctc gttttgcgca atgttggtca tagcactcag
     6841 cagcagcaaa ctcaatatga gcggggataa aaatctcatt tcacaaattt atcaaatata
     6901 tttttaaaaa tagaggaaat gcactttcaa tccgttttcg gaattccatt ccagtgcggg
     6961 ggcggggcat ttcagaaagt tgaggctgcg cataagcagc ttccagccac ggcctcgcac
     7021 aggtatcttg ctgaaatcaa tgtccggtgc caggtaccat tgactgtatc gattgaaaaa
     7081 aggaacagca ttgccatcga cttcggctgg attgtaatct ccgccgatca ttccatcggc
     7141 accgtagccc accgccacat tgagccatgc gggaaatcgt gattcttttt taaggcattc
     7201 cttaatgttc atgctcagcc agtatgtctg gccattatag tctttaagcg gctgctggag
     7261 caggttttcg ccaagcagcg acggattgta ctgcgcgtaa ctggtttgat gaaatcccat
     7321 ttttactgac agcaggtttt cttttccggt catttcattc aaagcaaaga gcgagactcc
     7381 aagcgtgttt gcacacatgt cgcccatcga aaacccccag ccgtcggaga agccatcgag
     7441 aacttcaata acggtcatat aggaaaaaga acagatagta ctgatgcgtg ccgattgacc
     7501 gcgtgtgtag ccggcgttcc gatacagatc ggactgcaca cgggtgagat gaaaagcaga
     7561 ggtcatgtgg ccgagcttgt ccattccctt ccattcgtcc atgtcgttga aaaaatgaaa
     7621 atgcccggta gaataatcgc tgtaccacag gaagctgagg ccggtgagcg agccggcata
     7681 gaatacaccg gaaaaggtgt tgatctgcca ttttttgaag aggggctttg ttgtatcgcc
     7741 gggtgaaaat aaattagaat ttttttcaac cttttcaacc tgagaaactt tttcaaagtt
     7801 tgaaactttg aaaaagttag gttcaatatt attttcagcc ggcaaagttg cggcgaaaaa
     7861 aatggtgagt agtatgaggc aaatccgctt cagcatggtg cttcggcggt ttacaggaat
     7921 gcttttattt ccttgaaata accttctttg tcggtggaga aagtcgaagt atgatcagct
     7981 tctttcacta tataggtttt ggtgattccg ccgcggatac cgcttatttt tttcaccata
     8041 tcgctattat ataccggatc gattttgccg tagataatca gaattccatt cagactggcg
     8101 cctttggctt cgagagcgaa tttcggctcg agctgatttt tatcgaagcc aagaggaagt
     8161 tttacttctt tgccggtagt tgttttaatc gctttctgca catcgtcaag ggtggagtag
     8221 ggtgagtcgg caatgatttt tgaaatttcg ctgcagcgtg ttgctcctat ggcaagtgac
     8281 aatgcggcgc cgacaccttg tccatacaaa catactgtgc ggcattttga atgatatttg
     8341 gaaacataat cgatggctgc attcaggtcg agtgcaaact gcgtgtagat gtagaattcc
     8401 ggattgatat tgaaagcatc actctggccg aatccacgat aatcataggt gagaacattg
     8461 tagcccagcg atacaaagtt ggtggctact tcaatcagat cggccatgtt gccttcgccg
     8521 ttgtcgctga ggataatcat tttgtaggaa acttcagatg gcttgaaaag ccatcctttc
     8581 aggttcaggt tgtccgaagt tttgatggaa acttcttcgt aattgagccc gtaatcggca
     8641 ggggtaactg cataggtgag tgacgggtca agagccgatg cccacatagc gctgaaaagc
     8701 agggcgataa ataaaagact tctcatagga tatttttttt gatggaccgt ggttacaaaa
     8761 aattatgccg ctttaagcat cgagatactg acggccttcg tttttaagaa aaatcaatgc
     8821 tttctgaaga agaccttctt ttgttttaag cgattcttcg aagatggatg tactcaggtc
     8881 gatggcgctg gcattgggct gcatggttgc acctgctgtg ttgatgaggc gaatgacgtc
     8941 ttctttggcg tttttcacca tatcccaggc ctgattcgaa atatatacct gttgcgataa
     9001 gttgtgctcg aactccatgc ggatggtttg tagtagcaac gcctgcagtt cttttgaact
     9061 catgccaacc tgattcatcc gcagcacgat gctttcagga ttgatgcgtt cgagaaacaa
     9121 ggccagccgc tcataggcct gcaaacggat gggcagcgaa acgcgcttct gctcgaagcg
     9181 ggcatccagt tctttctttt tctgttcgtt ttcgagataa cttttgagaa caaagaaagc
     9241 agtgagaaaa accatgagtg ccggcagcac aattttcgct aaatcaaggg caagttccat
     9301 ttcaatttct atttaaggca aaaataaaga attatttgat gtgaaaatat ggaaatgtgg
     9361 gaatatgtaa ttaggcatta ggtgacaggc cttatttagc ttcgctctat ccgccgcggc
     9421 ggacgctggg acaaattcca catacagccc gacgtctgcc ttgccaatca gataaaattc
     9481 caggtcagca ggttgtagtg tcaaccttat catgctgacg ctaaaatgtc gggttgacgc
     9541 acaacgcaca gcgtaattca caacgtttag gctggcagtc tagctaacgg cagaaaacca
     9601 cttgtcaccg atgtcactct gttacccctg tcacccctgt aacgactgta acccgtaacg
     9661 tttgtaacgc ttgtaacgac tgtaacgatt tccgcttcca gcggaataaa ttccacgact
     9721 gtaacgtctg taacgcgtaa cgaaaaatca ttcaacaaaa tacttcccaa acccgctggt
     9781 gctgtcggtt tttagggtgc catcgggagc gctgtaaatg aaaaatgctt ccatttcctt
     9841 attctgttca agaattattt tggctttatt aatacccata accataaatg cggtggcgta
     9901 ggcatcggca tccatgcatt tttttgtaat cacggttaca ctcaaaagcg agtggctgac
     9961 cggatatccg gtatgcggat cgatggtgtg tgaatatttc acgccgtctt tcacatagaa
    10021 tttgcggctg ctgcccgatg tggcaatgct gatgtcggtt atggcgagtt tgcggaaaat
    10081 ttcctgttcg gcaaattcgt tttcggccgg gcgttcaatg cccaccaccc atgaagctcc
    10141 atcgggcttt ttgcctgaaa cgcgcacctc gcctccgatt tcgaccatgt agtcatgcac
    10201 acctttgctt tcaagaaatt cagctatgag atcaacggaa tagccctgcg cgatggcatt
    10261 gaggtcaatc tgaatgccgg ctgaatcaaa aacaagatga ttacgtgaaa ttgaaatttt
    10321 ctgatagcca accagttgct tcagcgaatc gatggtagtg gaatccggat cagtgctttt
    10381 ttggcggccg aagccccatg cgcggaccag cggcccgacc gtgatatcga agtaaccatc
    10441 tgttttttca gatatttcga gggacttatt gagttgtgta attaattctg cgcttagcac
    10501 cgggttttca cctctgttta tgcggcaaat caatgaagaa tcattgtaga gtgaggccat
    10561 caggtcaaaa gcatgcagaa tgctgtcaac cgactccttt ttcacctgtt ccggcgagcc
    10621 ataccatttt atgtgaaacg tagtcccctg cgcgtttccg gaccactcaa ccaacgatgt
    10681 ctgaggctta tttccgcaag aaacaatcaa catgaccagc accggaaata ataatttttt
    10741 cattttttct gcgaacataa cattttttat tctaatttcg tacaaagaat gtttttgttt
    10801 gtagtataaa ccaaattccg gatatgaaaa aaattctgac actttttgga tctgttgtgt
    10861 ttgcagcaat gtttgttccg atgtttttca gttcgtgtga accagaggaa gatactgaag
    10921 ttccggaacc gacatcgact tttttctctg ctgtaatcgg agacaataac aagtcaatta
    10981 ctcctggagt gaatggatat cttgtagaac cgtatgatac aggttattct gatgcaagcg
    11041 gaacacatta tatctcaggt attcgtttgt atcagtccgg ttcgggttat tatgtttcat
    11101 cgcgtgaaga atttaaaatc gagcttgtga atttatttga ttcagcagct accaatcgcg
    11161 atagtctttt tcatgcatat ttatctcagg caaccttgcc ctggtacaat cctacacagg
    11221 ttgctgatag ttcaaaacac acaggtatgc gcatattgtg gcggacagcc gaaggggatt
    11281 ggtatgcaac tgatgaggca gctcagagtg gtacaatgac agttgacaca accaccgatg
    11341 taacagttcc tggcggcatc agtacgcatg aggtttatct tcagtataat tgcacattgt
    11401 atgagaaaaa tggttcaggg tcgcttgttc ttaccgaagg tcgcggtcgt ttctcattca
    11461 tcaattcaat gttctattaa aatatttttc ttctgaaccc ggcttctggc cgggtttttt
    11521 ttagtctttt ccattaatga aaaaacagct gtaactttct tcaatctgtt tttcggtgct
    11581 atcgttcagt gatgaaaaaa aatcaagacc ggtcagttct tctacccggt ctattgtttc
    11641 gcagtatgaa agatagtttt tcaatccttc actgtttgga ataataaaac caatagtata
    11701 aaacttatct gttttttttc ccagcagcac tttatagcag gcatcaggta tagcaacttt
    11761 gttttctccg ataaatgagt atgccgtatc ataaaaaatg ggtccagtaa tgataaagag
    11821 tgtatcaaac aacagccccc agtttcgtat gcgttgttcg agtgaattcc agatgccgcg
    11881 gttgaatccg ggctcctggg gactcatatt gctcatcaga aaactctcac tcatggcggt
    11941 actgtcgaaa cccatgtccc cagcaggaca cagatggccg cggtcataac cggatccttt
    12001 gtagtctttt aattctgccg aaccggtttt tatttttttg tcgggtctga aatcatctga
    12061 gcggcttgcg accttgtttt tcaatcgttc aggtgtaatg aaatgaaata cccaatcggc
    12121 ttgctctgtt ttttcgttat aacaaagcga aaagtactga tgctgtatca atgatgctga
    12181 atcgcataca gggtgaagct gtgcgttcaa cggcagaatt gagaaagaga tgattattgc
    12241 gacaaaatat tttttcagca acatgcgatt cagcattcaa tcaattgcat cagcatggaa
    12301 tgaatttcag cggggtctgg cactggttca tttatctgta aatcagtcaa tccgaactcc
    12361 ggtttcgaaa gcatatagcg atacatttca tatacttcat tctgaaaaga aaagtcagct
    12421 tcgtgaatat ctgcttttcc gtcaagatag tcgcgatcgg ctcctgaacg ttgattcaga
    12481 acattacctc tgcagaatga gagcggcata tgaataaaaa aagtctgatc gggaaccggt
    12541 aaatgaaaca cattgtattc tgtattcagt atccagtgag ccaattcgga cttttctttc
    12601 tgagaattta ttttggctcc ctggaaagcg atattagaaa gtacataacg gtcagcgata
    12661 acccatttgc cttcgttgag cagcgcagct atggctggcc cttcgttgcg tcggtcaccg
    12721 gcaaaaagca tggctacaaa ccatggatcg acttcggcaa gagacccgag ctcgcctttg
    12781 agaaagcgct tcagcattgg tccgtagaat gggctgctgc tatcggttct tgggaaatga
    12841 ataaattccg catcgatgcc tctgttgcgg attgcgtcca gcaaaagtgt ggtctgagtc
    12901 gattttccgc taccatccag accttccaga actatgaatt ttcctttgtt catcccaaat
    12961 tatgctttac atttgcgaag atattaattc tatggaatca tcggcaaaaa aaatattatt
    13021 gaatatacgt ggcagtttga tagaatttca aacacctgca gtgatgggaa taatcaatat
    13081 cacgtccgat tcttattatt cagcaagcag aaccgaggat gaaaaacggg catatgagcg
    13141 ttgcggaact ttgcttgatg agggcgccgc tgttattgat gtcggagctg taagtaccag
    13201 accgggttct gattatccgg gtgttgaaga ggaactgcga cgactggcaa gtgtttttcc
    13261 tatgcttaga aaggaatttc cagatgccat tttttcaata gatacttcaa atgctgtagt
    13321 tgcgcgccag atgatcgatt tgggagctga tatcattaat gatatttccg ccggagcgca
    13381 tgattccgat atgtttgcgc ttatcgctga aaagaaatgt ccttacataa tgatgcatat
    13441 gcgtggctgg cccaaagaca tgcagaagtg taccaagtat gccgatgttg taaagagcat
    13501 caatgattat tttgcacaaa aaattgaaaa actgcaacag atgggcgttt ccgacgtttt
    13561 tcttgatccg ggatttggct tttcaaaaac attggaacag aattttttaa ttttaaaaaa
    13621 tctacaaaca tttacttttc atgataagcc actggttgtt ggtatcagtc ggaaatcgat
    13681 ggtgtataaa gcattgaaaa gcacccccga agatgttttg cccgcaacta ttgccttgca
    13741 aaccattgcc ttattaaaag gtgcatcaat actgcgggtg catgatgttg ctgcggcagt
    13801 gcaatgtgtg caggtgctgg gattgatgaa tgaataaaat tatttcatca tcatttgttg
    13861 atttctttgg atacattgtg ttcccggggc tgtgccccga gccgttggcg ctttttgtac
    13921 tgacagctga agatgtcagc tatgttaaat acctcaacct caacctcaac ctcaaccttg
    13981 acctcaattt tgacctcaga tatttcttac tgcgtcgtcg ttgggagtta tgctgattac
    14041 cggaatctgg ctgtttttta tgatgttcga tgcggttgat ccaacaaagg tatgtcgcca
    14101 gtcctgttcc tgctgagtca taatcatgac aatatcggat tcagtatccg acgagtattg
    14161 aatgatgcgg tctgcaatgc ttgtatttgc tgaaaagtta taaataattt cagtggtgca
    14221 agccacattg ttttctgtaa gaaattctct ggttttattc attttttcag taaactcctt
    14281 ttctttgctg aaattgtcgc gggtaatgac tgtcagcagt tttattgttg agccataata
    14341 gcggctgaaa agaactgcta ctgcaatttt atttctgatt tgttttgaaa ggtcgagggg
    14401 tagcagaatt ttatcgaatg atgtttttac gatactgttg attgtaagaa cgggacagtt
    14461 ggccattctg acaacctgcg cagtgtattt cccgatggag ttttgcaggg ggcttgtttt
    14521 tcctgatttt cccatgacaa tcaagcgggc atgggttttg gcagcaaaat cgacaatttg
    14581 ttcatagaca ttaccttcga gtacggcata atccacatcg tggctgaact tttgagaaaa
    14641 atgtgttgca agtcgtctga tttttacgat aatatcttcg tcggccccca catatgaaac
    14701 tgaggactgt tgatcctgat aggaaattga attccgctgg ttgataacat gaagcagtgt
    14761 catcgatgaa ttcatcaggc gtgcaaggta gtaggcctgc tgaatctgga tatcagtgcg
    14821 ccggccaaaa tcaaccggaa ttaaaatatt gtcgtactta ctttccattt ttgtacattt
    14881 gtatgcaatg aatggctaaa ttaagaaaaa taatgaagaa aattgccgaa tctgaattga
    14941 tccttcgcga agatggagct gtttatcatc tgaatctgaa gcccggcgaa atagccgaca
    15001 atattattct tgttggagat ccggggcgtg tagagctgat ttcatcactg tttgacaaag
    15061 taacggtgtc aaaacagaac cgtgaaatat gcacccgaac cggcacttat aaagggaaaa
    15121 agataagcgt gatcagcact ggaatggggc ctgacaatat cgatattgtc attaatgaat
    15181 tagatgcctt ggtgaacatt gatttgaaaa agcggatgcc caaaaaagaa cgaaaatcgc
    15241 tgaacctgat tcgtataggt acttccgggg gcattcagaa aagtattccg gtgggatcat
    15301 ttcttttttc aagtcatggt atcgggactg atggattaat gcatttctat gcaggaagca
    15361 tcaaaaaacg ttgccctgaa atcgaactgg cgctcgccaa tcaagtgggt tggcccggaa
    15421 cctgggctgc tccttatgtt gttgaaggca gtgctaagtt gatcaaaaag ttatcaaccg
    15481 ggcatcctac cggtatgacc atgacttttg gcggatttta tggccctcag ggtcgtgagc
    15541 tgcgactggg cctgcattat cctgaattca atgatttggt cgccgctttt gatcataagg
    15601 gattgaaagt taccaatttc gaaatggaaa cctcagcttt gtatggtctt gcaaaaatgc
    15661 tgggacacaa tgcctgcacg gtttgtgttg tgattgccaa cagagccgat aaaactttta
    15721 ttaaggatta tcatccaccg atgaaagagc tgattaagtt ggtgcttgat cgtatctgat
    15781 cctcatcaag tattaaatgc aggtgcatga ttcaagatgt tttttgcatg ttttaatgcg
    15841 ttggttctgt tgtgtaattt cggtatacag gaaaatttga ttatttttgc aattcaaata
    15901 caacaacaat gagtaaacag caaaatgtgc tcccggaaaa gaaacagacc tttgtttttg
    15961 gccgcatgaa ctatattctt atgctcatcg ggctgggagt gatgtttttg ggttatatac
    16021 tgatgatggg tggtggatcc gaagatccga atgttttcag cgaagaactt ttcaacacac
    16081 agcgaaccac cattagtcct attcttatta ttgctggatt tgtcattgag atttttgcca
    16141 ttatgcaccg cccgaaagca aaagacaata gcgaagaatg agttggtttg atgcgattat
    16201 tatcgctatt gttgagggtc tgacagaatt cctgccggtt tcatccaccg gtcatatgat
    16261 tattaccgaa gcattacttg gacataagcc cgatgatttt tcaaaggcct ttattgttaa
    16321 tattcagttt ggtgccatat tggctgtggt ggttatgtat atccgccgtt tttttcagtc
    16381 gtttgatttt tacttaaagc ttgcagttgc atgcatcccg ctggtgatcg catttttgct
    16441 cgaagcgttc attgatcaga tgctcgaaag cgtattggtc gtggccattg ccttgtttgc
    16501 cggtggtatt gtgcttgtgt ttgttgaccg ttggtttaaa aatcctgaaa aagctgaaaa
    16561 agttttatca tcgaaaaatg catttgtcat tggccttttt caaatcattg ctgtcattcc
    16621 tggtgtgagt cgttcggcag caactatcat tggcgggttg tcacagggct tgagcagaaa
    16681 acaggcagct gaattcagct tttttctggc tgttccgacc atgcttgccg cctcggcata
    16741 caaactgtat aaaaatcatg aaattcttgg ctcggtcgaa aatctgcaat tgctggccat
    16801 aggcaatctg gtggcatttg tagtagccat tcttgctatt aagggattta ttgctttttt
    16861 gaaccgatat ggtttccggt ttttcggata ttacagaatc attctcggtg gtgttctcat
    16921 agccctcatt ttggccggat ataatttaag tgtggtgtaa atgtttgatt ttgcttcggg
    16981 tgaaatactg ctttttgata agccatacac atggacttcg tttgatgttg ttcatctcat
    17041 taaaaattgt ctgaagaaat caatgcctga gccggttaaa gtgggccatg ccggcacatt
    17101 ggatccgttg gcaaccggaa tgctggtagt gcttacaggt aagaaaacac gtgaaatgga
    17161 aattattcag gcgcaggaaa aggaatatac gggtacattt accattggtg ccacaacgcc
    17221 ttcttacgac agggaaagag aggttgatca gacattttca atcgatcata ttactgaaga
    17281 aatgatcgtc gaagctgcaa agacttttgt tggtgaaatc atgcagctgc cgcctgacca
    17341 tagtgctgta aaagttgatg gaaagcgggc ttacgagtat gcccgaaaag gaaagtctgt
    17401 tgatattaaa ccaagaccta ttactatttc ggaatttgaa ataacccgtg ttgcgcttcc
    17461 cgagattgat ttcagagtta cttgcagtaa gggaacttat attcgttcgc tggcccgcga
    17521 ttttggcgag gccctgaact caggcgctta tctgagctcg ctgtgccgca cgcgtattgg
    17581 cgaataccgg attgaagacg ccatgcatcc gaaagaattc actgctttga ttgagaagat
    17641 gaagcaagac gggatgctgt agaattccgc ataaagcagc acacacaatg acaaacattg
    17701 aatggctttt ttccagacgc ctggattttg tataaaaaaa gccggcagac gccggcttat
    17761 aatgaaagag atattttatt ctctcatgat ggtcagtgtt ccatgatggt cttcgtattc
    17821 ttcgttgcca tagttgactc ttaaaataat gaagtaagtc ccatcggcaa aattttcacc
    17881 atcccagtca ttcatataat ttgaattttc gtagattttc ttaccccagc gattgtacac
    17941 cattaaaatg ctgtttgggt agaaaggaag attgaaaact tcaaaaacat cgttgattcc
    18001 atctccgttg ggtgtgatga tgttggggat attcagatcg catgcaataa cgcttacaac
    18061 gacagtatca gattttgaat cacaaccggt tacggttaca gaatacactg cgggacctac
    18121 aggaagacct tctacgtgaa gataaggaga ggtgcttccg gttgaccaat gatatgtata
    18181 ttgcggtgta gattggctta catcgaaatc gaggaattca tgtcggcaga tttcctgatc
    18241 cgggccaaga ttgacaatgg gtgatggaat gacattcacg tttattgaat ccacatcgct
    18301 gcaaagactt gagccgccat atgaaacggt aacagagtaa acccctgttg aggaaatggt
    18361 tgtggtttgc gttgtggcac cattcgacca taaaaagtca aaccccgggt tctgagcgtc
    18421 taatgtaatg ctttcggttt cgcacaggtc gggtcccaga ttgacactgg cccatggaat
    18481 catggtgata ttaatggtat ctgatccata gcaggcaata ttgccgtttg agtttgttac
    18541 ggtaacggca taattatcgg ataaatcggg gttaatggtt tgagtgatgg caccattcga
    18601 ccagaagtac tggttcccgg aaccggcatc gagagtaaga acgctgcctt cgcaaacagt
    18661 ggtatcggca ccgagatcgg gttggggatt ggcaagaact tccacattca atgttgtatc
    18721 gtatgaacag ccaaaagcat cccagactgt ggctgtatag gtgaagattc ctggactttg
    18781 aggtgcaata aatgcggtgg aatccgaggt tgaggtaatg ctggggccgg cccatgaaac
    18841 agaatcaatt ggaaccgtat atgaccaggc gccgctctgg gtctgattga gcaacgtgag
    18901 tgaccaccag aaaacatatc cgttatcttg tccccattca tcgcagattt caatgctcca
    18961 ggtgccgttg agcggacatc cgaccaatgc tgaaaacggg gtttcggtgg taatatatcc
    19021 ggtgttgttt atggtgtcag tagcgggaat tggggatgta cctgcatcaa gtgtattcaa
    19081 agtcccctgc tggggatata cttctgacca gcaataggtc cagccggttc ccggaggatt
    19141 ttgagccggg tcacagtcat cataagttgt tgagtttgaa attccaaggt ctgagccccc
    19201 actattgtca tagctgtcca tctgcacggt tgtgccgttg ggacaaataa tactgaatgc
    19261 gaggtccccg gcaaaagtgt gctccatatt gatgcagatc gatgcaatat cggatgcgct
    19321 ggtaattgaa gatccgggag gaaaagtgga gaatgtaaca ggtgtgtagt aacagttggt
    19381 tgaacaatat ggtccgtcgg gaataaacgt agtactgtcg tacagctcag tggcaatctg
    19441 aaacgaaccg gttggggcca ccacaatggt tgacgttgca ttttcgccaa caacaagatc
    19501 aatggtatcg ccggcgcaga tatcgggcaa tggttttatg tcgataatag gattgcctga
    19561 aacacggaca cgcagggcaa gcaattgggt atttgtacat ccgcgggtat cgatgacagt
    19621 cagaaatacg tcataaccca tgacagaagt atataaatgg tcaatcacac ggcctgtatc
    19681 gatagttcca tcaccaaagt cccatatgaa aagcgttgtt gacgagtcct gatggtacaa
    19741 aaggtcgttt tcagggaata ctccggggcc tgcgcccatt gcagcaaaag taacaatgtc
    19801 gccaaagcag atatcaacat aattgctgtc gtttggcaga ggaagcatct gcagggtatc
    19861 tactgctgcc agtatttcct gacattgagg gatgcagcca atggagcagt tccatccatc
    19921 ggaaaccgtg ctgccattgc ttttgaatct gaaagtaaga tcgcctgaaa cattggcaat
    19981 ggaagcctgc accatggtat tcgggctggt caggggatta ttgttattgt attttccgat
    20041 aaccggagag gttgtgtttg ggccatcgta aataatgagt gtgtcacccg gttcaatatc
    20101 gaaagcagca aagctggctt ttatatgagt gtttaaaaca tcgctgctgg caaatgtgat
    20161 ccagcgatcc tgattgtttg tgtaatcgcc ggaagcggtg ccatcatcat aaaaccaata
    20221 tgagcagcca ttttgagtgg tgttgtgggt tgcggcatcg agattgacaa tggtttgccc
    20281 gatagcggac aaactgaaaa aaatgaatga tacagcaata acaagtattt tcatgataat
    20341 atctcctatt tgccggcttt aaatttgttt tcaatataag aaagagaatg cagcttgata
    20401 tagaaatccg cattttcaat tttcacccaa agggtttcat ttttgtccca ttgcaatccg
    20461 gttgttaaaa tattgaatgc tttgagattg ttcaatgcgg ccagattgtc ttttgaaaga
    20521 ataatagatc tggtttcgaa tggattgatg tactcagcaa caacatcatt tttccatatt
    20581 tcaaatgcgt tgtcgagaaa gaaattgtaa tacctgatag agtcggggta ttgatttgac
    20641 aatgtttgta cttttgaatt tccaaaagct tcaatcaatc gggcatcagc ctgaacggaa
    20701 ttttgcgcaa tggctggtgt aaccagtaaa aaacacgcaa tcagtagaat aattttattc
    20761 atcagtagaa atttttatct atcgttagac aaatgtgcga aaaaaaagtt taacaaacgc
    20821 ctttttttta aaaaaaggac acattttttt taaaatgata gacaaaaaga tcattttgtt
    20881 agttgcacaa gagtgctcca tttttcttca gggatcttgg ctccaatggt agtgattact
    20941 tcagaagcaa tcactgagcc ggttctgccg cattcatagg cgctttttcc ttttgacagg
    21001 gcgtataaaa agccggcagc atataaatca cctgctccgg tagtgtcaac cggaattatt
    21061 ttttgtgtag gcacagctgt gatttcgccg ttcatggcaa caaatgaacc tcccgggcct
    21121 gtttttacgg ccgcaatatc tacccgctga cttaaatatt ttgccgactc ttcgggttcc
    21181 agtccggtca acgctttgct ttcctcttca ttggcaaaaa tgatatctgt gtagagatca
    21241 atcatctcat tgaggaaatc gcggtgttct tcaaccacgt tgtaactggc aagatcaagt
    21301 gatactttga gaccaagctc ttttgcagtt ttaattgaat gcgaaatcag tttgtaattc
    21361 tgcaacagat aaccctctat atgcagtatg tcatagtttt tgagtacatc agcatcaatg
    21421 tcgatatcct gaagttcgca ggcggcgccc aggtaagtgg caaaagtgcg ttcaccatcg
    21481 ggcgaaacca gtgccagtgc gagcccgcta ggcgttgcag attgaataag atgggtctgg
    21541 atcccgcttt taaccatgtc tttttcgaaa aaatcaccca gatcatcttt cccaactttt
    21601 cctataaaac ctgttttcat gcctaatcgt gcaagtccat gaatggtgtt ggccgcagag
    21661 ccgccactgg ccagagtgcg tttgaaatgc tttgttgcat cggatgcgcg ctgcgacatg
    21721 gtcgcatcaa ccaattgcat actccctttg ggaagattga ggtttcttaa tatgtcgtcg
    21781 ctttcgatct gagtcatcag gtctgtgagt gcatttccga taccaataat tttcattgtc
    21841 aaaaattttt tttgcaaatt tcggaaaaat attgccatga taaaataatt tattattttt
    21901 gcactcccaa aatatgggaa acatattcct ccctagctca gttggttaga gcatctgact
    21961 gttaatcaga gggtcgctgg ttcaagtcca gcggggggag caaaaacaga gtgagagtga
    22021 gagcgagtga acagcgagcg accactctca ttctgttttc tgatatctcg cactcaaact
    22081 cgcactctgc attaatcctt cattcatgtt ttcgcttgca aaaacatgaa tgaaatcaaa
    22141 taaaacaacc catgttcgac ttcaaaaaac tcattgtata tcagaaagca cgtgattttg
    22201 ccctaactac aactgaactg ctcgccaata acaaattcga caaggcggta aatgatcagt
    22261 tgcgtagagc gtcgctcagc atcatgctga atattgcgga gggttcatcg cgtttcagca
    22321 acaaggatcg gaaatacttt atggttgtgg cgcgggaaaa tgatttataa gtttaagatc
    22381 tgccagtgca atctgatttt gggggatcgg gttctattat tttggaatta atggcgcgga
    22441 tttgtcagaa tcaattaatc atgtgctctg acaagtaaac tagtattaac cattaacgga
    22501 cagctgaaaa ggattattga ttgtcgtctt tccgaaaatt cgcaaaagtg caacggagtc
    22561 ttctttatta acagacgctt tctgaatctt tttcagttga taaggttgat taattgatgg
    22621 aattcggcta gcgaaaaaag gccacaatga atcactctcg taagttttag atgaatcagg
    22681 gaaatcaatt ataggtttaa ttaactcttg cgtcctgaat tcatcagaat attgaaaata
    22741 ccaaacagat ttctcatact tcagaaatcc aatggtcata tttccatatt tcaatgtaaa
    22801 ttttccggaa atatcttttg gattgtcgtt aaactccatc ccctcttcct tccagaaaat
    22861 tttcctgaga attttattca tggtgcaaag ataatacatt tttcagacat tcaatgcgat
    22921 ataacaaggt ctgctcaatc agtttccttc tgaaatcact catcagagga gcaaattcat
    22981 tgttgatcag atttgtaatt gccaacgtgt tatcagtatt gttcagtatg tcagcagcac
    23041 tcttatatgg aaaatccggt tgcgacatgg aatgcttaat aagatcgaaa tgatttattt
    23101 tcccaatatt gtcccatcca atttttgggt agctatgttc agcatatttc tttattgctg
    23161 gcattcttag atgtggagat ccatatttgt cctgcaaatc cttttccgat gtattccagt
    23221 acaatccccg ggcactatca tatattggtg aaaattccgg attgctattt ctgatatctc
    23281 tgacaatacc ccaattataa tagtgtctat cgttatttcc agttattgcg tcaaataaaa
    23341 gcaataaata aaaactactc atgagttggt gaaaatgatc tgggaagaaa tgcctgaggg
    23401 tttgctgtgt aaactgcact gtaaaaaact caggagatct attttggttt tcaacctcgg
    23461 acacaaaatc tttgtcattc aggtagccgg cataaagatc ggctccatgc tcaagtactt
    23521 gcgtacatct gttggaaaga aaatactctg acagaaaacg aatttgtcct ccaagccttg
    23581 ccagacacga ttttgccata ttcaaaccca gcacttcgcc aattctattg agtaggtgtt
    23641 ctgtgatagc ttctgtcgga tagtgtttat gcccgtgttt tgctatatat cgcggccagg
    23701 aatcgggatt attctttcga ccacgaccat attcatagcg agaaatgaaa tctttcggcg
    23761 catcaccatc aattgatgtg tttttgataa tgaaataatg gtgttttttt acaaatggaa
    23821 ttgcaccggg ttttggattg tctcccgtgt agtcaataat ttttaaggga tggatggttt
    23881 tgcgttttct catactatta catcaggcaa atatactaaa tcctgaacat cttttcaatt
    23941 gatttttttg ttttctgcaa tagaattcat actactaaaa atttcgggtt ctacaattag
    24001 cgatgtgtca aaattagtgt agaggagttg tgggtgtgtg agaattttga atcagtgaaa
    24061 attaattcta aaatagtcaa ttgagcc
//
LOCUS       DAOPKE010000039        23741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2262_length_23741_cov_14.401461, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000039 DAOPKE010000000
VERSION     DAOPKE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2262_length_23741_cov_14.401461"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..386)
                     /locus_tag="PKY63_06710"
     CDS             complement(18..386)
                     /locus_tag="PKY63_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00340.1"
                     /translation="MKRLFLFFIVVLPYTIKAQTVVFDQYQYWYYRDRLQYFVYPGTE
                     KGNSLIATLRNPTSGRPYKTIKYGQTFKSMGYYLGVLATEYKLLKDNGQIADANATLS
                     ELNLALDALIRMDKCEDDIP"
     gene            complement(389..940)
                     /locus_tag="PKY63_06715"
     CDS             complement(389..940)
                     /locus_tag="PKY63_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00341.1"
                     /translation="MKTKSIKNVFTIALSRWLSGLTILFAIFTMVACGRYEEGPCISF
                     RSVKSRLEGQYIVTFFEKNGLDVLPEWKENFDWKISFANDRESEEYNFYANGKYKIDT
                     SFYYLNQHGRYELPLGKEQISFYLEIPYSSVSVITPEYGFYPFIQNETIVVDISRLTK
                     DDLWISHRSGNDFYEIHLKKIKY"
     gene            complement(960..1121)
                     /locus_tag="PKY63_06720"
     CDS             complement(960..1121)
                     /locus_tag="PKY63_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00342.1"
                     /translation="MAGNCLFVFALTLLSRTVFGTLLRKIEELTLYMLELKKENEELR
                     KMIEEKESN"
     gene            1521..2024
                     /locus_tag="PKY63_06725"
     CDS             1521..2024
                     /locus_tag="PKY63_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF00343.1"
                     /translation="MDRQEYNRCVELYSDAVYRFILKNTADSEDSADIVQEAFVRMWE
                     KSETVSFEKAKSYLFTSAYHIMIDYFRKKKRYSTGFDMEDDSHCWHPAANEKTSSFDI
                     KAILNEALDKLPPMQKSVILLRDYEGYSYEEIGEITGLNESQVKVYIYRGRIALKNFI
                     KSPDLIL"
     gene            2042..3040
                     /locus_tag="PKY63_06730"
     CDS             2042..3040
                     /locus_tag="PKY63_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00344.1"
                     /translation="MERIVDYYDGKLSQSESDALMAFLEINPELYEEFMMFGEALPET
                     AAEDTLKAPASLIEQLKNIPDEVFVLSDEMLVAYAEKDLDAYTIKKVEEAIAQNPQRK
                     RDLEILSAARYEADEAIAFPYKNKLLRREPIVFSLRRMMYYTSAAAAIFILAFFLWPA
                     VNHESGIQSDSHNIAEMNLQITNSENTAENNSEKNSGNNNNSHIQNSDIATQLADNST
                     NASKNHEYIAPLSPRPAKQLKETAQIAPGNIEENRNEYIDIYNYIEMKNAALAESNVK
                     TGTLNAWKEWGKGFLNGESSLTDTPADITLRDVATFSYNNIAQFASESLSLSQRDR"
     gene            3089..4477
                     /locus_tag="PKY63_06735"
     CDS             3089..4477
                     /locus_tag="PKY63_06735"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPF00345.1"
                     /translation="MKTLALLTLMTVAFSAAIAQQPIDSFTKINSTGDIKITIKNGDT
                     HSVTFPGTGKAPEGLIYNVTGGVMNIDATKVKESVNIIISAPLINEISLNSVTELHTE
                     GIFITPQLKLTSDGAAEYHMELQCETLSLDFKGASEAWIEGTTNTLIAQLSGASKVHA
                     YDLNTKNAEIKTDGASSVNLTVTENLKATGSGASEISYDGTPANVDLNIEGVASIKKR
                     NGEDVGSAVPDGYEIIKTDENNEDDEDDCSRDYNGHWAGVDLMMNSFVSKYGDLNTAP
                     GYDFMETDFSNSIGAQINFMEFNIPFVEREKMSMGITTGLGWSMLNYRLDKKTRLIND
                     SSILMAYADSASNTIRSKLTTSYLILPMMLEINTSSVKKENGFHFGIGAFGGVRVGSH
                     SKVVIDDRGDKDKFKSNGNFHLNPFKYGVMARVGWGPLNLFANYALSEMFEKNEGPAV
                     YPVEFGLSFDFN"
     gene            4611..6059
                     /locus_tag="PKY63_06740"
     CDS             4611..6059
                     /locus_tag="PKY63_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPF00346.1"
                     /translation="MKYFTVVVLLFAFINVTGQTLLKQSQAFAMDIPATVIIDNGQKV
                     LVGSYDTKMYLINAATGNVEKEIAEHKGFVLSLAYCPKTNTVASAGWDQRIILWDAGS
                     MTKKLDIQAHTDRITSLSFSPDGTRLVSGSEDKTICVWDVMTGGEIFKITDHKDAVTT
                     VVYSKDGNSIASGGWDKLVMIHSAHDGKLINTYKGHRSSINSVDFSYAGDMLVSGSDD
                     NSITIWRTDSSKTIAKFDFFKLPVTNVYFFPGDKYIFCADGSGELKIYNVQNRSMLTQ
                     KNIHSGAVKNIFINPSIGLMASTGSEKIMKLWNINEYLYYDCVKEKSKALQDMAKPKG
                     EFETTEMYERRMKEYDIKKSALVAECAKESELIRKAKQEELDQKTLSTYSYVYFQIQN
                     LGTYDADKQEYPFVFNGQAGMVKLSIEEAKSLKINVSNAKIKAIKRVVNGNTEYINTE
                     LIHPVSGRSIAFGRQITATEDPILGKFLQNKH"
     gene            6140..6619
                     /locus_tag="PKY63_06745"
     CDS             6140..6619
                     /locus_tag="PKY63_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPF00347.1"
                     /translation="MKTSDQYIIHFSGLAAGTHDYSWDIDGKFFEADNYLGITDASIQ
                     VTCRLEKSSRLMELDFSGKGELTLPCDRCLGPLAVKINAKRKVIVKESEAENSDSDDI
                     FFVGRQDYEFSAENWIKEMILLSLPMRNVHREGKCDTEMIEKLKKLSDDNGLKPLNE"
     gene            6633..6812
                     /gene="rpmF"
                     /locus_tag="PKY63_06750"
     CDS             6633..6812
                     /gene="rpmF"
                     /locus_tag="PKY63_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPF00348.1"
                     /translation="MANLKYRWSKTRTRNRRANFKAEAPTFAVCSNCGTPVLYHHVCP
                     ECGFYKGKLAIEKNS"
     gene            6834..7832
                     /locus_tag="PKY63_06755"
     CDS             6834..7832
                     /locus_tag="PKY63_06755"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPF00349.1"
                     /translation="MSKVRAAITGIAGAVPDYILTNEELSRMVDTSDEWIMTRIGIKE
                     RHILKEPGKGSSDLGAAAVKKLLEKTNTKPEEIELVVCATVTPDHQFPATSSIISDKV
                     GIKNAFHFDLNAGCSGFLYGLATVTAYIESGRVKKAILVGAEKMSSIVDYTDRTTCPI
                     FGDGSGAALIEATEDDFGIMDIKLESDGVGRIHLYQKAGGSVWPSSAETVANREHFIY
                     QEGQPVFKWAVSKMADTAVDVMERNGLTPETLQWLVPHQANLRIIEATGSRMKLPKEK
                     VMVNIHKYGNTTAATLPLCLLDYEKQLKKGDNLILATFGAGFTWGAIYVKWAYDTK"
     gene            7943..8110
                     /locus_tag="PKY63_06760"
     CDS             7943..8110
                     /locus_tag="PKY63_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF00350.1"
                     /translation="MAYVISEDCTACGTCIDECPVEAISAGPIYKIDPDVCTDCGSCA
                     DVCPVEAIHPE"
     gene            complement(8284..8589)
                     /locus_tag="PKY63_06765"
     CDS             complement(8284..8589)
                     /locus_tag="PKY63_06765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF00351.1"
                     /translation="MAKQEIDIRKIEDVAQRLRAIAHPVRIKIISMLEKQHEMNVTSI
                     YERLDIEQAAASHHLNILKSKGVLLSRRDGKNTYYSLKPEAVSNLLHCISVCCAKDS"
     gene            8739..9905
                     /locus_tag="PKY63_06770"
     CDS             8739..9905
                     /locus_tag="PKY63_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF00352.1"
                     /translation="MIFFPEYMLMPDSSIRSGMAVLTNPNGSIKDVFEAKENLYLNEK
                     TYHCCGLLLPGLINSHCHLELSWCRGLYPEKSGMEAFYGAMSGVHSRRPADQIILANI
                     QHAMEELYNQGVVAIADISNTPLTLSAKTNSNIYFHTFLEVFGTNENDAKYCFNDILT
                     LQKTFAENKQNCSLSAHTLITLSQTLMSLLMTRIEEEKTIHSIHFLESIEEKQYFEQM
                     RPILNIHGNNFAKSNYKSASNAACKLMPTQNRVMFVHNTFAGKENIEEILHHFSNPWF
                     CFCPASNQYITGQLPDVPMIQSLTPDILIGTDSLSSNHELNMFAEIDILLDTYATLKP
                     ETLLAAATLNGARFLGIDKQFGSIEKNKKPGLVLLENYQPGLRKFSSLTVKRLL"
     gene            complement(9909..10436)
                     /locus_tag="PKY63_06775"
     CDS             complement(9909..10436)
                     /locus_tag="PKY63_06775"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HPF00353.1"
                     /translation="MKNLFVLSFVLFVFVAAKAQVQVDPDTKKIMYREVVTMDGTKDS
                     LYNRAIGWINSFYKNPQAVTTLRDPENGVILCAHRFAMKETDKDGNVMTSNVIVEYKL
                     RIEAKEGRYRYTFDEFRKKDVSMFPLERWLDKADPQYTPKCDEYLAQVDVKIKEIIAS
                     LKKGMERKVVKTDEW"
     gene            complement(10528..12441)
                     /locus_tag="PKY63_06780"
     CDS             complement(10528..12441)
                     /locus_tag="PKY63_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPF00354.1"
                     /translation="MENRARILSVLQQYWGYKMFRPMQEDIILSVLNGKDTLALLPTG
                     GGKSITFQVPALAKDGLCIVISPLIALMKDQVENLKKRNINAYAIHSGLNQFEIETIL
                     NNCIYGEVKFLYVSPERLETEMFLLRLPKMKICLIAVDEAHCISQWGFDFRPPYRNIS
                     KIRLLIPDVPVVALTATATPAVVDDIMEQLAFREKIVFKQSFYRSNLVYNVIQEEDKT
                     GRLLQLLSEYKGTAIVYVRNRRKTKEYADFLQRNKISAHFYNAGLLPNERDKRQADWI
                     AGKVRVMVATNAFGMGIDKPDVRLVVHMDIPDCIEAYFQEAGRGGRDGNMSWAWLLWN
                     RADIIEADKNVDASFPDPQFISRVYNALGNYFQIVTGTGKLSTWNFELTDFCNRYSLP
                     SVETFNSVRILEKEGYVSLSEAIHTPSKVKMQADKNDVYRFQVENRELSPFIDLLLRM
                     YSGLFTGFVRIDEFSIARKMNADKLIVIKGLKQLDKLGLISYEPASDSPRVTFLSDRI
                     DHRDVIFSEEHYYSRKKEAVNRLESMKTFLSGTTRCRSQLLLDYFGETQSRRCGMCDI
                     CEKRSETGLSEYKFNEVLDRVKPALREAPMPYDKVLSMFGAMDSEKATKAIRWMMDNG
                     KIGMDDDGNLYWK"
     gene            complement(12505..13263)
                     /locus_tag="PKY63_06785"
     CDS             complement(12505..13263)
                     /locus_tag="PKY63_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF00355.1"
                     /translation="MKKIVLVSGATSGIGASCAHKFASAGYRVILCGRRSERLSALKQ
                     KLVSLYGENFFTLTFDIRSREQVFNAINSLPAEWENTDVLINNAGLALGLSSLQDGSI
                     DHWEQMIDTNVKGLLYLTKAVVGQMIERKSGHVINIGSIAGRQAYANGNVYCATKAAV
                     DSLTKSMRIDFLPYNIKVSQVAPGAAETEFSIVRFEGDQQRAADVYKGYTPLSADDVA
                     DAVLYLASLPPHVNVNDMLLMPTAQACAGIIHKE"
     gene            complement(13266..14576)
                     /locus_tag="PKY63_06790"
     CDS             complement(13266..14576)
                     /locus_tag="PKY63_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00356.1"
                     /translation="MIAAEILYCIVIVAIMSLLILKLRAFSLPGISRWLVLSAFYLKV
                     LAGLGLTYVYTSYYSGRNDADIFRYFDDSKVMYDAVKSLPADYFTMLSGIGDDQQYFF
                     DNYYSKMNNWYLKYQTVMYNDSHTVIRINAFFRLFSLGFFQVHNILFVILGFIGLLAL
                     YKTFIKYFSSKSLALYFAVFLIPSVVFWSSGILKESLLIFAMGLLFYSYQQLLFISFN
                     VYRVVLLLFSLLLMYFTKTYVLAVLVPVLLANTWIVLTNNRHAFAKQLVTLLVFINGA
                     IIVGNLFPDFNVFQLITNKLHDFTNLALYNNAGSMIDPIPIEPHFLSFLKYSPLAVLT
                     TLFRPFPSDVNSLIMVPVLIENIIIAATVFLAVVFHEKKLQYIRIIYLCLFFVIVLSV
                     ITGLTTPVLGALARYRVPLLPFLFIMLFLIIDFNKIARFLKIKN"
     gene            complement(14576..18253)
                     /gene="purL"
                     /locus_tag="PKY63_06795"
                     /gene_synonym="purI"
     CDS             complement(14576..18253)
                     /gene="purL"
                     /locus_tag="PKY63_06795"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPF00357.1"
                     /translation="MLTFFKQESEGLIYAVQSRTQLGEKDVEKFRWLFGSCAEISTEG
                     IKGDFIGPRREMVTPWSTNAVDITVNMGVQGIERIEVFRPKKGKMDPMLEMEYKSLTP
                     DMFNQERQPEPIKFIDHLEEYNEQQGLALSFEEVRFLKYISKKIGRKLTDSEIFGFSQ
                     VNSEHCRHKIFNGDFIIDGREMEMSLFQWIKLTTKEHPNDVISAYKDNVAFIDGPEIE
                     LFGPQSDDQPSEFVTRKVESVISLKAETHNFPTTVEPFFGASTGSGGEIRDRMAGGKG
                     CMPLVGTAVYMTSKPRLEEPVANADGVIPERRKFLYQTPEEILIKASNGASDFGNKFG
                     QPLIVGSLLTFEHQEGKTLTGFDKVIMLAGGVGYGVKTQAIKGKPAPGNLIVLMGGDN
                     YRIGMGGGAVSSVETGKYTNSIELNAVQRANPEMQKRVYNVVRALAESNDNPIVSIHD
                     HGAGGHLNCFSELVEDTGGTIFIDKLPKGDPTLSDLEIIGNESQERMGLVIREKSLPL
                     IEKIAARERAPLYVVGEVKGNHKLIFEGDDNRKPFNLKLQYLFGCTPKTVIEDRRMDQ
                     HYNEPTYELRGFNNYLNDVLEQEAVACKDWLTNKVDRSVTGKVALQQCCGEIQLPLNN
                     LGIAALDYSSKSGIASAIGHAPVAGLIDAGAGARLSVAESLTNIVWTPLKNGLQGVSL
                     SANWMWPCKNPGEDARLYDAVSALSRFCIALGINVPTGKDSLSMTQKYPDGSIVKSPG
                     TVIVSATAQTTDFTKAVKPVLATEIDSTLIYIDFTKGPFALGGSVFYQTLNSVGQQTP
                     DVADPEYFANAFNAIQVLISKNKILAGHDISAGGMITALLEMCFANPHVGLDINISTI
                     KENDIPAILFSEKPGVLIQVKDTKFVVDYLYNKNISFFPIGRVIGERVLKIIKNVQYE
                     YFEIDKLRDVWFRKSYLLDRLQTKPQLAEKRMVNYLKQPLKYEFPAFFDGTFSEVKKR
                     NVKAAIIREKGVNGDREMAYSLYMAGMSVRDVHMTDLIEGHEDLHDINMLVFVGGFSN
                     SDVLGSAKGWAAGFLFNSKAKKALDDFYARPDTLSLGVCNGCQLMVELGVFNDRKKKI
                     PHPEMHWNESHKFESGFVGVQVLPNESVMLKAFEGSKLGVWIAHGEGRFRLSGQEKDY
                     NIPMKYLYEEYPANPNGSDFNAAALSTHDGRHLAMMPHIERSVFPWQWAYYKPGITSD
                     VVSPWAHAFVAARQWIEAKSK"
     gene            18452..19027
                     /locus_tag="PKY63_06800"
     CDS             18452..19027
                     /locus_tag="PKY63_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF00358.1"
                     /translation="MNNSVEFTDQHRELIEQCRRGERNAFREVYRLYSKSMFNVALRM
                     TGMAHIAEDILQEAFTDAFSTLNKFRHESTFGAWLKRIVVFKCYDYLNSKSAGFEFLE
                     DMESFDQPDQEPDFNEEEMAREVKRIKEAVQLLPAGSRTVLTLYLFEGYDHEEIGQIL
                     NISESTSKTQYMRARLKLKELLTQSPLHNER"
     gene            19017..19649
                     /locus_tag="PKY63_06805"
     CDS             19017..19649
                     /locus_tag="PKY63_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00359.1"
                     /translation="MKDNLEKFISQNRHQFDDEEPSPGLWDRIENSVPAPARMVRMRP
                     LTYAASIAAACIIVWIVASVVMNQQSHDADKVLSENIIRPELKVAQHDTVFVPVVVNP
                     EKNTTNTNVTNASDKNLYAEITQYYDAEIAQRKAKLQSVSSGNKDIMYQVQEELAMID
                     TLNAQTQRELGNGMNIGLVMEQLVQNYRQSIDILDMMLEQVNEEYAQINE"
     gene            19675..20727
                     /locus_tag="PKY63_06810"
     CDS             19675..20727
                     /locus_tag="PKY63_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00360.1"
                     /translation="MSLCILATEAVCAQSFTVNKKFHGSYPAAANSEVSIDNKYGNVS
                     IIPSDGDSVVFDAVVEVTDKYIVEAEEQLAEINVRMGASGYYIYAATEFNGNRNDFKT
                     DWKMMAGAVFSSKKTVRIDYIVKVPSGVSLKINNSYGNLIMPDHSGILNIDLSSGNLT
                     AGILSGKTTITLNGGKSQIRALTDATIKTDMNEMQIESVSSAFFDSRGSQFTIQKAGT
                     ITVDSRRDKWFIGNATQISGSGSFSNFTISRLMKDCNMKTFYGNLTLSAVDSGFHSIN
                     LIAGSSDVSIALPPGVVPQFNCDQKKTRAILPAKLNLKTETIDEKTQQYRTTNVVENA
                     TSFVNLNVSGGSVNVY"
     gene            complement(20763..20909)
                     /locus_tag="PKY63_06815"
     CDS             complement(20763..20909)
                     /locus_tag="PKY63_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00361.1"
                     /translation="MNNALENNDASWIRDLWIGSFTGICGSALMWDNMHRCDLWPHFG
                     RLTK"
     gene            complement(20902..21498)
                     /locus_tag="PKY63_06820"
     CDS             complement(20902..21498)
                     /locus_tag="PKY63_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00362.1"
                     /translation="MKTKVLFSVLLFFLMQLCFAQRLSSSFGLLYIIQYPDGRNISNS
                     ALPNAEGKISYSFFNATHLKMNVGVGVATHNFLLKDISENQTYGYLRAEPFASLSLMG
                     CLSEESDFPIHGIYAGIRAGFFNQWNFNPSWQVGLISKPFGKVQFYAQFSKMLCYSIP
                     DNNYLLFDFTDNYYPLGNERWSITAGVQFGFGRKKKNE"
     gene            complement(21500..21982)
                     /locus_tag="PKY63_06825"
     CDS             complement(21500..21982)
                     /locus_tag="PKY63_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00363.1"
                     /translation="MKKIIFFISPLLFFLSCSKESVLPDEYQYLIGTWTLKNCIVSAD
                     FYTPHYPYSNSDTLPPDSFGFDQSIKIDDNSIVFYSENTIIQIVDNYTIVEVNTGTYG
                     CEYTISFYLQYKDEIGCKKSTSVIYIPAKDSLRIYYRLYDGSSGTYYGPSYSYNYIRE
                     "
     gene            complement(21979..>23741)
                     /locus_tag="PKY63_06830"
     CDS             complement(21979..>23741)
                     /locus_tag="PKY63_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00364.1"
                     /translation="EQICNYYNRMHIGWEVDNIVEKAEQEGLFIHFNTAIQYYFLKPD
                     RIFWDWAAYGEAGLPEECDNSLDHGYCYNKDLGLTDPKSFFLDYRAIKYYTNRLRYMI
                     ARWGYSTNISAFETINEINGTCGTKEMYYNATEQKCKDVPESGYAPYNNEAFFRQKVY
                     AWQSSMINSMTTYMGYNQHLTSVNYLTEGPNISDDHTYYLNSLDIITQQNYRSFPDIY
                     NSTVTIVNDLHDNSNASSDGWPYCDKPFIHGEYGSGTNQGYYQCDNDASWIRDLWIGS
                     FTGICGSALMWDNMHKRELWPHFGRLRAFVAGYDFDESNGDQKGWIPYHDFDEMHYEN
                     WQRKPGIVDLFYLRSPDKLNAVGVLANRTYNFYTQWDVAKCGAFVWESDSTRWQFELN
                     TPPNIIQWDDQAYNSAKTAYNINEALQVDVKSKKRYIITYIDPNNLQVVHTQEKYNLT
                     GKLRLDFPYLTGTTIRPILLVCVEPKNNKSLEQFSDSTRKYFEDISAEIQEKNSKVTA
                     LSRAMVSPNPTDQAVLITLPVSTENCRMILFSCSGMMIKSNVFSGQTFLLDVGDCDAG
                     VYILQLNYNQKVETVKLIIQ"
BASE COUNT         7508 a         5205 c         4536 g         6492 t
ORIGIN      
        1 ggtttaatgc cactatctca tggaatatca tcctcgcatt tatccatccg tattaatgca
       61 tccagagcca gatttagttc tgaaagcgtt gcatttgcat cagcaatttg tccgttatct
      121 ttcaatagtt tatattcagt tgccaaaact ccaagataat accccattga tttaaatgtc
      181 tgaccatact tgatcgtttt atatggccta cctgaagttg gatttcttaa tgtagcaatc
      241 aaggagttgc ccttctcagt gcccggatac acaaaatatt gtaaacgatc tctgtaatac
      301 caatattgat attgatcaaa aactactgtt tgagctttga tagtgtatgg caaaacaact
      361 ataaagaata aaaacaatcg cttcatgtct agtattttat tttttttaaa tggatttcat
      421 agaaatcatt cccgctcctg tgtgaaatcc ataagtcatc cttggtcaac ctggaaatat
      481 caactactat ggtttcattc tgaataaacg gataaaatcc gtattctgga gtaattaccg
      541 aaacacttga atatggtatc tccagataaa aagaaatttg ttcctttccc aaaggtaatt
      601 cgtaccgacc atgctgattt aaataataaa atgaagtgtc aattttatac tttccatttg
      661 cataaaaatt gtattcttca ctctcccgat catttgcaaa actaatcttc cagtcaaaat
      721 tctctttcca ctccggtaaa acgtctaatc cgtttttctc aaaaaatgtt acaatatatt
      781 gaccttcaag tcttgatttt acacttctga aactaataca cggtccctcc tcatatctgc
      841 cgcaagcaac catggtgaag atagcaaata atattgtcag tcccgatagc catcgggaga
      901 gagcgatagt gaaaacattt tttattgatt ttgttttcat ggctttcaaa ttttaagagt
      961 taattagatt ccttctcttc aatcatcttc ctcagctcct cgttttcctt cttcagttcg
     1021 agcatataga gggtgagttc ttcaattttg cggagcaggg tgccgaatac agtgcgtgag
     1081 agcagtgtga gagcgaaaac aaaaagacaa ttccctgcca cttccaattc tgcccaaatg
     1141 ccaggcaggt gggcgcttca gaaaattgaa tttcataatt gttttgtaat tgaaactgca
     1201 agaatatgaa ataatttttt atttgtcaaa tatttttaat tttttgtatg tattattcaa
     1261 tttataaatg atgatatcaa ccaatttata agtcgtatcg catttcatta ataatgtagc
     1321 acatgaaaat cgcagcaaaa atcaaaccct gcatacaagg aatttgttac aatgaaagcg
     1381 gggcatcatg cattgcgctc tgcaagcact aacgtcgcct ttctacttta aaaacttgat
     1441 taactttata aacttccatc tcgctgtaac atttcgtaac tttactccgt cataggcctg
     1501 taaagtaacc aaaccacctg atggaccgac aagaatataa ccgttgcgtt gagctgtatt
     1561 ccgatgcagt ttaccggttt atcctgaaaa ataccgccga cagcgaagac tcggccgata
     1621 tcgttcagga agcgtttgtc cgcatgtggg aaaaatcgga aacggtttct ttcgaaaaag
     1681 caaaatccta tctgttcact tcggcctatc acatcatgat cgattacttc agaaagaaaa
     1741 aacgctactc aaccggcttc gacatggaag acgacagcca ctgctggcat ccggcagcca
     1801 acgaaaaaac gtcatcgttc gacataaagg ccattctgaa cgaagcactc gacaaactgc
     1861 cacccatgca gaaatcggtg attctgttgc gcgactacga agggtattcc tacgaagaaa
     1921 taggcgaaat aaccggcctg aacgaaagcc aggtgaaagt gtacatttac cgcggacgca
     1981 ttgcattgaa aaatttcatc aaaagcccgg accttatatt atgatcagcc acaaaaacta
     2041 tatggaacgc attgttgatt actacgacgg caaactgtcg cagagcgaat ctgatgcact
     2101 catggctttt ctggaaataa atccggaact gtatgaagaa ttcatgatgt ttggcgaagc
     2161 tctgccggaa accgcagctg aagatacttt gaaagcaccc gcctcattga ttgagcaatt
     2221 aaaaaatatt cccgacgaag tttttgtgct gagcgatgaa atgctggtgg cttatgctga
     2281 gaaagatctt gatgcctaca ccatcaaaaa agtcgaggaa gcaatcgctc aaaacccgca
     2341 acggaagcgc gatctggaaa tcctcagcgc tgcacgctat gaggcagacg aagccattgc
     2401 gtttccgtat aaaaataaat tgctgcgcag agaacctatt gtgttttcgt tgcgcagaat
     2461 gatgtattat acctcggcag ccgcagccat tttcattctt gcatttttcc tgtggccggc
     2521 agtaaatcat gaatcgggca ttcagagcga ctctcataac attgctgaaa tgaatctgca
     2581 aatcacaaat tcagaaaata ctgcagaaaa taattctgaa aaaaattccg gcaataataa
     2641 caattcacat attcagaatt ctgatatagc aacacagttg gctgacaaca gtacaaatgc
     2701 atcaaaaaac cacgaataca ttgcaccgct atctccacgt ccggcaaagc aattgaagga
     2761 aacagcacag atagcacccg gcaacatcga agaaaacaga aacgaataca ttgatattta
     2821 caattacatt gaaatgaaaa atgcggctct ggctgaaagc aatgtaaaaa ccggaacact
     2881 caatgcatgg aaagaatggg gaaaaggatt tctgaacgga gaatcatcac tcacagatac
     2941 acctgccgat attacattac gtgatgtagc cacattcagc tacaacaata ttgctcaatt
     3001 tgcctctgaa agtctttctc tgagccaaag agacagatag ctgtaacttt cggaacaccc
     3061 tcttcgtctt attacaaaat tacacactat gaaaacttta gcactcttga cactcatgac
     3121 tgtcgcattc agtgcagcca ttgcacagca acccattgac agtttcacta aaatcaactc
     3181 cacaggtgat ataaaaatca ccatcaaaaa cggcgacacc cacagtgtaa cttttcccgg
     3241 aaccggcaaa gcacccgaag gactcattta taatgtaact ggcggcgtta tgaatattga
     3301 cgccactaaa gtaaaagagt ctgtgaatat aatcatcagc gcacccctaa ttaatgagat
     3361 ttcgctgaat tctgttacag aattacatac tgaaggcatt ttcattacac cacaactcaa
     3421 actcacttcg gatggagccg ctgaatatca catggaactg caatgtgaaa cactgagtct
     3481 tgattttaaa ggtgcctcag aagcatggat tgaagggact acaaatactt taatcgcaca
     3541 attaagcgga gcatctaaag tgcacgcata cgatctgaac accaaaaacg cagaaataaa
     3601 aacagatgga gcttcgagtg taaatctcac tgttacagaa aaccttaaag cgacaggcag
     3661 cggggcttca gaaatatcct acgacggaac tccagccaat gttgatctaa atattgaagg
     3721 agttgccagc ataaaaaaac gcaatggcga agatgtcgga tctgctgttc cggatggata
     3781 tgaaataatc aagaccgatg aaaataacga agacgatgaa gatgactgtt cgcgtgatta
     3841 caatggccat tgggctggtg tcgatctgat gatgaacagt tttgtttcga aatatggtga
     3901 cctgaatact gcacccggat atgattttat ggaaaccgac ttctccaatt caatcggagc
     3961 acaaattaac tttatggaat ttaatattcc ttttgttgaa agagaaaaaa tgagcatggg
     4021 aataaccaca ggactgggct ggagcatgct gaactaccga ctggataaaa aaacacgcct
     4081 gatcaacgac tccagcatac tcatggcata tgccgacagc gcttcaaata ccatccgcag
     4141 caaactcacc acttcatatc tgatattgcc aatgatgctt gaaataaaca catcaagcgt
     4201 taaaaaagaa aacggttttc actttggtat aggtgcattt ggaggagtca gagtcggctc
     4261 acacagcaag gtggttattg acgatcgcgg cgacaaggac aaattcaaat caaatggcaa
     4321 ctttcatctc aatccgttta aatatggagt gatggctcgt gttggttggg gaccactgaa
     4381 tttatttgca aactacgcac tctccgaaat gttcgaaaaa aatgaaggac ctgcagtcta
     4441 tcctgtcgaa ttcggccttt ctttcgactt caactaatat ccagacttga atcatgactc
     4501 gttggcctgc ctgagcgcag gccattttta gttttatcag ataaatatcg gtcaaaactt
     4561 tatttttttc tatatttgac gtctaatagt ttcagaaatc acaattttct atgaaatatt
     4621 ttacagttgt tgttctgctt tttgcattca taaacgtaac gggtcagact ttactaaaac
     4681 aaagccaggc gtttgccatg gacattccgg ctaccgttat tattgacaac gggcaaaaag
     4741 tgctggtggg atcctatgat accaaaatgt acctgattaa tgcagccaca ggcaatgttg
     4801 aaaaagaaat agccgaacac aaaggatttg tcctttcgtt ggcctattgt ccaaaaacca
     4861 ataccgtagc atcggctgga tgggatcagc ggattatttt atgggatgcc ggatcaatga
     4921 ctaaaaaact tgatattcag gcacatacag atcgcatcac ctcgctcagt ttttcacccg
     4981 acggaacgcg attggtcagt ggcagcgaag ataaaaccat ttgcgtttgg gatgtaatga
     5041 ctggtggaga aatttttaaa atcaccgatc acaaagatgc agtaacaacc gttgtttaca
     5101 gcaaagacgg caacagtatt gcttcgggtg gctgggataa actggttatg attcattcag
     5161 cccatgatgg aaaactaatc aatacataca aagggcatcg cagttccatc aactctgttg
     5221 atttttcata tgccggcgac atgctggttt ccggttccga cgataacagc attactatct
     5281 ggcgcacaga ttcttcaaaa acaattgcaa aatttgattt tttcaaacta ccagtaacca
     5341 atgtgtattt tttcccaggc gacaaatata tcttctgtgc cgatggcagc ggagaattga
     5401 aaatttataa tgttcagaac cgctccatgc tgactcaaaa aaacatacac agtggtgcgg
     5461 ttaaaaatat ttttatcaat cccagcattg gactgatggc cagcactggc tcggaaaaaa
     5521 tcatgaaact gtggaacatc aacgaatatc tttattatga ctgtgtaaaa gaaaaatcaa
     5581 aagcattgca ggacatggca aagccgaaag gagaattcga aaccacagag atgtacgagc
     5641 gcagaatgaa agaatacgac attaaaaaaa gcgcccttgt ggcagaatgc gccaaggaat
     5701 cagaacttat ccgcaaagca aaacaggaag aactggatca gaaaacactt tcaacatatt
     5761 catatgtcta ttttcaaatc cagaatctgg gcacctatga tgcagacaaa caagagtacc
     5821 catttgtttt caacggtcag gcaggcatgg tgaagttaag cattgaagag gccaagtcgc
     5881 tcaaaatcaa tgtatccaat gcaaaaatca aagccatcaa gagagttgtc aatgggaaca
     5941 ccgaatatat taacacagaa ttaatacatc cggtcagtgg cagaagcatt gccttcggac
     6001 gacagattac cgccacagaa gatccgatat tgggtaagtt tctgcaaaac aagcattagg
     6061 caaagaatac ctcgtcaaat atttaggttt tgtaatccac aaaatattca tatctttgca
     6121 gcccgtaaaa atggggttcg tgaagacgtc tgatcaatac atcattcatt tcagcggatt
     6181 agcagcagga acgcatgact actcctggga tattgatgga aagttctttg aagcagacaa
     6241 ttatttggga atcaccgatg cctccataca ggtgacatgc agattagaaa agtcaagcag
     6301 actcatggag cttgatttct caggcaaggg agaattgacc cttccatgcg atcgctgttt
     6361 gggaccactg gcagtaaaaa tcaatgccaa acgaaaagta attgtcaaag aatctgaagc
     6421 cgagaattca gacagtgacg atattttttt tgttggcaga caagattacg agttttcggc
     6481 agaaaactgg atcaaagaaa tgattttact atcgctacct atgcgcaatg tacatcgtga
     6541 aggcaaatgt gacaccgaaa tgattgaaaa gctaaaaaaa ctgtcggacg ataacggact
     6601 gaaacctctg aatgaataaa aattaaattg caatggcaaa tctcaagtat cgttggtcaa
     6661 agaccagaac ccgtaacaga agagctaatt tcaaagccga agctcccaca tttgcagtct
     6721 gttcaaactg cggaacgccg gtgctttatc atcatgtatg tcccgaatgt ggcttttaca
     6781 aagggaaact ggcaattgaa aagaactcat aattcattaa tccttcaagc atcatgagta
     6841 aagtaagagc agctatcacc gggatagccg gtgctgtgcc tgattatatt ctgacaaacg
     6901 aagagctcag ccgcatggtt gatacttcgg atgaatggat tatgacccgg atcggaatca
     6961 aagaacgaca cattctgaaa gaacccggta aaggatcgag cgatctcgga gcagctgccg
     7021 ttaaaaaatt acttgaaaaa accaacacaa aacccgaaga aatcgaactg gtcgtttgtg
     7081 caacagtaac acccgaccat cagtttccgg caacatcgag catcatcagc gataaagtcg
     7141 gcataaaaaa cgcatttcat ttcgatctga atgcaggatg cagcggattt ctttatggat
     7201 tggctacagt aactgcctac atcgaatcgg gccgtgtgaa aaaagccatc cttgtcggtg
     7261 ccgaaaaaat gtcttccatt gttgactata ccgaccgcac cacctgcccc atttttggtg
     7321 atggtagcgg cgcagcattg attgaagcaa cagaagatga ctttggcatt atggacatca
     7381 aacttgaaag cgatggagtt ggccgcattc atctctatca gaaagctggt ggtagtgtat
     7441 ggccttcatc agccgaaact gttgccaatc gtgaacactt tatttatcag gaaggtcagc
     7501 cggttttcaa atgggctgtg tccaaaatgg ccgataccgc tgtcgatgtg atggaaagaa
     7561 acggacttac ccccgaaaca cttcagtggc ttgttccgca tcaggcaaac cttcgcatta
     7621 tagaagcaac cggaagccgc atgaaacttc ccaaggaaaa agttatggtg aatatccaca
     7681 aatatgggaa taccaccgct gccactcttc cgctttgttt gctcgattac gaaaaacaac
     7741 tcaaaaaagg cgacaacctt atactggcca cattcggtgc tggtttcaca tggggcgcca
     7801 tttacgtaaa atgggcttac gacaccaaat aatattttca tcaaggcgcc atccggcgcc
     7861 ttttcttttt gttttttaat attattgtcg ttattaaaac aataatgcat atctttgcag
     7921 cctaagttaa aaaaacagag ttatggctta cgttatttca gaagattgta ctgcttgcgg
     7981 tacctgcatt gatgaatgtc cagttgaagc aatcagcgcc ggtcctattt ataaaattga
     8041 cccggatgtc tgcaccgact gtggctcttg tgctgatgtt tgtccggtgg aagctattca
     8101 cccggaataa taatccgctc acagaaaaaa tctgaaggct gcctttgtgc agcctttttt
     8161 attcttcaaa aaacaatttc ccgacacatc aaaaccaatc caaaatatac gattttgaaa
     8221 atttgcacaa tatacatcca taccaaccaa ttatgattgg taatgcctgc agaaaaatca
     8281 ttctcacgaa tctttcgcgc agcaaacact gatgcaatga agcaaattac tgactgcttc
     8341 aggcttcaga ctgtaatagg tgtttttgcc atccctgcgg gaaagcaata ccccttttga
     8401 ctttagaatg tttaaatgat gcgaagcagc cgcctgctca atatccagcc tttcataaat
     8461 gctggttaca ttcatttcgt gttgtttttc aagcattgaa ataatcttga tccggactgg
     8521 atgggctatg gcccttaatc tttgtgctac gtcctcaatt tttctgatat caatttcttg
     8581 ctttgccatc tataattatt taatattaat ggacaaatat atatattaat tttctaaata
     8641 atgattttta caaaaaaaat tgataccgac aggttatttg ccgatatttg ccttgttttg
     8701 ctaaatcagt ctatcacctt ttgtcaaaaa aatctgctat gatttttttt cctgaataca
     8761 tgctgatgcc cgattcttcg atccgaagcg gcatggctgt tcttacaaac cccaatggca
     8821 gcatcaaaga tgtttttgaa gcaaaagaaa acctctacct taacgaaaaa acatatcact
     8881 gctgcggact tttattgcca ggattgataa atagtcattg tcatcttgag cttagctggt
     8941 gcagaggttt atatcccgaa aaatcaggga tggaagcctt ctatggcgct atgagcggag
     9001 tccattcacg ccggcctgcc gatcaaataa ttctggccaa tattcaacat gccatggaag
     9061 agctgtacaa tcagggagtt gttgctatag ccgacatcag caatacacca ttaactcttt
     9121 ctgccaaaac caacagcaac atatattttc atacatttct tgaagttttt ggcaccaacg
     9181 aaaacgatgc aaaatactgt ttcaatgata tactgactct tcaaaaaaca tttgccgaaa
     9241 acaaacaaaa ctgctctctc tctgcacaca cactgataac actttcccaa acgctgatgt
     9301 cactgttgat gacacgtatc gaagaagaaa aaacaattca cagcattcat tttctcgaaa
     9361 gtattgaaga aaagcaatat tttgaacaaa tgcggccaat actgaatatt catggaaata
     9421 actttgcaaa atcaaattat aaatctgcat ccaatgcggc ctgcaagctc atgccaaccc
     9481 aaaaccgcgt catgtttgta cacaacacat ttgccgggaa agaaaatatt gaagaaattc
     9541 tacatcattt ttccaatccg tggttttgct tctgtccggc ttcaaatcaa tatatcaccg
     9601 gacaattacc tgatgtgccc atgattcaat cattaacacc agacatactg attggaacag
     9661 acagtttatc ttcgaatcac gagctgaata tgtttgccga aattgatata ttgctcgata
     9721 cttacgctac attaaaaccc gaaacactgc tcgcagccgc aacactgaac ggcgcacgat
     9781 ttcttggaat tgacaaacag ttcggcagca tagaaaaaaa taaaaaaccc ggattagtat
     9841 tgcttgaaaa ctaccaaccc gggctcagaa agttcagttc actaacagtg aaaagacttt
     9901 tataattttc accattcgtc ggttttaaca actttacgtt ccatcccttt tttcaaactg
     9961 gcaatgattt ctttaatttt tacatcaacc tgcgccagat actcatcgca tttgggcgta
    10021 tattgcgggt cggccttatc gagccagcgc tccaacggaa acatggaaac atcttttttg
    10081 cggaactcat caaatgtata acgataccga ccttctttag cttcaatacg taatttatat
    10141 tctacaataa cattcgatgt catcacatta ccatccttat cggtttcttt catggcaaaa
    10201 cggtgtgcac aaagaattac accattttca ggatctcgca gggttgttac tgcttgtggg
    10261 tttttataaa atgaattaat ccagccaata gccctgttat ataaagaatc ttttgtacca
    10321 tccatggtca ccacttcacg atacatgatt ttttttgtat ccggatctac ctgaacctga
    10381 gctttggcag caacaaaaac aaacaagaca aatgagagaa caaacagatt tttcataaaa
    10441 aaatttttgc agcgtgttac aaatataatt ccaaaaattc cgaaggacta aattaaataa
    10501 aaatatccaa tccgggaaag tcttttttta tttccaatac aaatttccgt catcatccat
    10561 gccaatcttg ccattatcca tcatccatcg gattgctttg gtggcttttt cactatccat
    10621 tgccccaaac atagacagca ccttgtcgta tggcatagga gcttctctca gagctggttt
    10681 cacgcggtcc aggacttcgt tgaatttata ctccgacaaa cctgtttcgc tgcgtttttc
    10741 gcatatatca cacataccac agcgacggga ttgtgtttcg ccaaaataat caagcaacaa
    10801 ctgactgcgg caacgagtgg ttcccgacaa aaaagtcttc atagattcga gccggttaac
    10861 agcttctttt tttctgctat aataatgctc ttccgaaaaa atcacgtccc ggtgatcaat
    10921 ccgatcactc agaaaagtaa ctctgggact gtcgctagct ggttcataag aaataagccc
    10981 aagtttatcc agctgtttca atcctttaat aacaatcaat ttatcggcat tcatcttacg
    11041 tgcaatagaa aactcatcga ttctgacaaa ccccgtaaac aggcctgaat acatgcgcag
    11101 caacaaatcg ataaaaggac tcagttcacg gttttcgacc tgaaaccggt acacatcatt
    11161 tttgtcggcc tgcattttca ccttcgaagg agtatggata gcttcgctga gcgacacgta
    11221 tccttctttt tcaagaatcc gaactgaatt gaatgtttca accgaaggca gagaataacg
    11281 gttgcaaaaa tcggtgagtt caaaattcca tgttgataat ttcccggtac ccgttacaat
    11341 ctgaaaataa tttccaagag cgttgtaaac acgcgatatg aattgaggat cagggaaaga
    11401 agcatcgaca tttttatcag cttcgatgat atcggctcta ttccacaaaa gccaggccca
    11461 cgacatattc ccatcacggc caccacggcc ggcttcctga aaataggcct cgatacaatc
    11521 aggaatatcc atatgcacca ccaatcgcac atcgggttta tcgattccca taccgaaggc
    11581 attggtagca accatcaccc tcactttccc tgcaatccag tcggcttgtc ttttatcacg
    11641 ctcattaggc aataatccgg cattgtagaa atgagcactg attttattgc gttgtaaaaa
    11701 atcggcatat tcctttgttt ttcttcgatt acgaacataa acaatggcag ttcccttata
    11761 ctcactgagt aattgaagca atcgtccggt tttatcttct tcctgaataa cattataaac
    11821 cagattgctc cgataaaacg actgtttaaa aacaattttt tcacgaaagg caagctgctc
    11881 cataatatca tcaacaacag caggagtggc ggttgcagta agtgctacca ccggaacatc
    11941 gggaataagc aaacggattt ttgaaatgtt gcggtaagga gggcggaaat caaaacccca
    12001 ctgcgaaatg caatgtgcct catcaactgc aatgagacag attttcattt tcggcaggcg
    12061 caaaagaaac atttccgttt cgagacgttc gggcgagaca tacaaaaatt tcacctcgcc
    12121 ataaatgcaa ttattgagta tagtctcgat ttcaaactga ttcaaaccac tatgaatagc
    12181 atatgcatta atatttcttt ttttcagatt ttcaacctga tccttcatca atgcaataag
    12241 cggtgaaatt acaatacaca agccatcctt tgccaatgcc ggcacctgaa aagtgatgga
    12301 ctttcctcct cctgttggaa gcaaagcaag cgtatccttg ccattgagca cagaaaggat
    12361 aatatcttcc tgcatagggc gaaacatttt gtatccccag tattgctgaa gcacggaaag
    12421 tattctggct ctgttttcca ttaaacaaat tcaaatataa aaagaaatat ataaacaaag
    12481 gaacgaaaaa gaaaaagaaa aaaatcattc cttgtggata attcccgcac aggcctgagc
    12541 tgttggcata agaagcatgt cgttcacatt cacatgtgga ggcagcgaag ctaaatacaa
    12601 gactgcatcg gccacatcat cggcagacaa aggagtataa cccttgtaaa catcggccgc
    12661 ccgttgctga tcaccctcga aacgaacaat actaaactct gtctctgccg caccaggagc
    12721 tacctgcgac accttaatat tatacggaag aaaatcaata cgcatgcttt ttgtgagaga
    12781 atcaacagct gcttttgtag cacaatacac atttccatta gcatatgcct gtctgccagc
    12841 aatagagccg atattgataa catgtccgct tttacgttca atcatttgtc cgacaactgc
    12901 ttttgtcagg taaagcaatc ccttaacatt ggtatcaatc atttgctccc aatgatcaat
    12961 actgccatcc tgcaacgagc tcagcccaag cgccagtcct gcattattga ttaaaacatc
    13021 tgtattttcc cattccgccg gtaatgaatt tatagcattg aaaacctgtt cgcggctgcg
    13081 aatatcgaaa gtaagcgtaa aaaagttttc accatacaaa gaaacaagct tttgttttaa
    13141 agctgaaagg cgctctgatc gccgtccaca aagaataacg cgataaccag cagaagcaaa
    13201 tttatgagca catgaagctc caatgccgct tgtagcgcct gaaacaagaa caattttttt
    13261 cataactaat ttttaatttt cagaaatcgt gcaattttat taaagtcaat aatcagaaac
    13321 agcataatga aaagaaatgg gagcaaagga actctgtaac gcgccagagc accaagtaca
    13381 ggtgtagtca atcctgtaat aacacttaaa acaataacaa agaaaagaca tagataaatg
    13441 atccggatat actgtagttt tttttcatga aacactacag cgagaaagac tgttgcggca
    13501 atgattatat tctcaatcaa caccggcacc ataatcaaag aattcacatc ggagggaaaa
    13561 ggccggaaaa gcgtggttaa aacagccaaa ggcgaatact tcaaaaaact cagaaaatga
    13621 ggttctatcg gaatcggatc aatcatgctt ccggcgttgt tgtacaaggc cagattggtg
    13681 aaatcatgca atttattagt gataagctga aagacattaa aatccggaaa cagattacca
    13741 acaataattg ccccattaat gaatacaagt aaggttacta attgttttgc aaaagcatga
    13801 cgattattgg tcagtacaat ccatgtattg gcaagcaaaa ccggcaccaa aacagcaagg
    13861 acatatgttt ttgtaaaata catcagcagc agactgaaca acaacaatac aacacggtaa
    13921 acattgaaag aaataaacaa aagctgctga tacgaataaa acaaaagtcc catggcaaaa
    13981 atcaaaagac tttctttcag aatgccagaa ctccagaaaa caaccgaagg aatcaaaaaa
    14041 acagcaaaat aaagagcaag agacttggaa ctgaaatact tgatgaaggt tttatacaaa
    14101 gcaagcaaac caataaaacc aagaataaca aaaagtatat tatgcacctg aaaaaaacca
    14161 agagaaaaca agcgaaaaaa agcattgatg cgaataacag tatgactatc attatacatt
    14221 actgtctgat acttcagata ccagttattc atcttgctgt agtaattgtc gaaaaaatac
    14281 tgctggtcgt caccaatacc gctcagcatg gtaaaataat cagctggcaa cgatttaaca
    14341 gcatcataca tcactttact gtcgtcgaaa tagcgaaaaa tatccgcatc atttctgccg
    14401 gaataatagg atgtataaac atacgtcaat cccaaaccgg caagcacttt aagataaaaa
    14461 gccgacaaca caagccatct gctgataccc ggcaaggaaa aagctctcaa cttaagtatc
    14521 agcagcgaca ttatcgctac aatgacaata caatatagaa tttctgcagc aatcattatt
    14581 tgctttttgc ctcaatccac tgacgtgctg caacaaaagc atgagcccat ggagaaacca
    14641 catcgcttgt tattccaggt ttataataag cccattgcca tgggaaaacc gatcgctcga
    14701 tatgcggcat catggccaga tgacgaccgt catgagtaga gagagctgca gcattaaagt
    14761 cagaaccatt tggattggcc ggatactctt catacagata tttcatcggt atattgtagt
    14821 ctttctcctg accagacaat ctgaaacgtc cctctccatg cgcaatccat acacccagct
    14881 ttgatccttc aaacgctttc agcattacac tttcgttagg cagaacctga actccaacaa
    14941 atcccgactc aaatttatgc gactcattcc agtgcatttc cggatgtgga attttctttt
    15001 ttctgtcatt gaaaacaccc aattccacca tcaactggca gccattgcaa acaccaagac
    15061 tcaatgtatc aggtcttgca taaaaatcat ccagcgcctt ttttgctttg gaattgaaaa
    15121 ggaaaccagc agcccatcct ttggcagagc caaggacatc gctgttgctg aatccgccta
    15181 caaacaccag catgttgatg tcgtgcagat cttcgtgacc ttcaatcagg tcagtcatat
    15241 gtacatcgcg tacactcata ccggccatat acagactgta agccatttca cgatcaccgt
    15301 tcacaccttt ctcacgaatg atagccgctt ttacatttcg tttttttact tcagaaaatg
    15361 taccatcaaa gaatgccgga aattcgtatt tcaacggttg cttcagataa ttcaccatac
    15421 gtttttcggc cagttgaggc ttcgtctgca aacgatccaa aagataggat tttctgaacc
    15481 aaacatcgcg aagcttatca atttcgaaat attcgtactg aacatttttg atgatcttca
    15541 gcacgcgctc gccaattaca cggccaatcg ggaagaatga aatatttttg ttgtagagat
    15601 aatcaacaac aaattttgtg tctttgacct gaatcaaaac gcccggtttt tccgaaaaaa
    15661 gaatggccgg aatgtcattt tccttaatgg tactgatatt gatatcgagc cccacatgcg
    15721 ggtttgcgaa gcacatttca agcagagccg taatcatgcc cccagcgctg atatcatgac
    15781 ctgcaagaat tttatttttg gatatcagaa cctgaattgc attgaacgca ttggcaaaat
    15841 attcaggatc ggctacatcc ggtgtctgct gtccaaccga attgagtgtc tgataaaaca
    15901 cactgccacc caaagcaaaa gggcctttgg taaaatcaat ataaatcagt gtggagtcaa
    15961 tttctgttgc aagaacaggt tttacggctt ttgtaaaatc tgttgtctgt gcagttgccg
    16021 acacaatcac tgttccgggc gatttcacaa tcgagccatc gggatatttc tgtgtcatcg
    16081 acaacgaatc ttttcctgtc ggaacgttaa ttccaagcgc aatgcagaat ctgctgagag
    16141 cgcttaccgc atcatacaaa cgggcatctt cacccggatt tttgcatggc cacatccaat
    16201 tggcactgag tgaaacaccc tgcaatccgt ttttgagcgg agtccagaca atgtttgtca
    16261 aagattcggc aacgctgagt ctggctccag ctcctgcatc gataagtcca gccacaggtg
    16321 catgaccgat tgcagaagca atgcctgatt tactgctata atcaagtgca gcaattccaa
    16381 gattattcag tggcaattga atttctccgc aacattgttg cagcgccact ttgccagtta
    16441 ccgaacgatc cactttattg gtcagccaat ccttgcaggc aacagcttcc tgttctaaaa
    16501 catcattcag ataattgttg aaaccgcgca attcatacgt tggttcgtta tagtgctgat
    16561 ccatgcggcg atcttcgatc accgttttgg gagtacatcc gaaaagatac tgcaatttca
    16621 aattaaacgg cttccgattg tcatctcctt caaaaataag cttatgattc cctttcactt
    16681 caccaacaac atataaagga gctcgttcac gtgctgcaat tttttcaata agtggaagtg
    16741 acttttcacg aatcacaagt cccatgcgct cctgcgactc attgccaata atttcaagat
    16801 cggacaaagt aggatcaccc ttcggaagct tatcaataaa aatggttccg ccggtgtctt
    16861 ctaccagttc gctgaaacaa ttcaaatgtc cgccggcacc atgatcatga atagaaacaa
    16921 tcggattgtc gtttgattct gccaaagcac gcacaacatt atacacacgc ttctgcatct
    16981 caggattggc acgttgaaca gcattgagtt caatggagtt ggtatatttt cctgtctcaa
    17041 ctgacgatac cgctcctccg cccattccaa tccggtagtt gtcaccgccc atcaaaacga
    17101 tgagatttcc gggcgcaggt tttcccttga tggcctgagt tttcacacca taaccaacac
    17161 caccggccag catgattact ttatcaaagc cggtcagcgt ctttccttcc tgatgttcga
    17221 atgtaagaag actgccaaca atcagaggct gaccaaactt gtttccgaaa tccgatgcgc
    17281 cattggatgc tttaattaat atctcttcgg gtgtttgata taaaaatttg cggcgttcgg
    17341 gaattactcc gtcggcattg gcaaccggct cttcgagccg cggtttcgat gtcatataaa
    17401 cagctgtgcc aacaagcggc atacatccct tgccaccggc catccgatcg cggatctcac
    17461 caccgctgcc ggttgatgct ccaaagaaag gttcaacggt tgtcgggaaa ttatgcgttt
    17521 cagctttgag cgaaataacc gattcaactt tgcgtgtaac aaattcagag ggctggtcat
    17581 cgctctgggg tccgaataat tcaatttccg gcccgtcgat aaaggccaca ttgtctttat
    17641 atgccgaaat tacatcattc ggatgttctt tagtggtaag tttgatccac tgaaacaagg
    17701 acatttccat ttccctgccg tcgatgataa aatcaccatt gaatatttta tggcggcaat
    17761 gctcggaatt tacctgagag aatccgaaaa tttcactgtc agtaagcttg cggccgattt
    17821 ttttgctgat gtatttcaga aatctgactt cctcgaaact cagtgccagc ccttgctgct
    17881 cattgtattc ttcgagatga tcaataaatt ttatcggctc cggctgtcgt tcctgattaa
    17941 acatatccgg agtcagactc ttatactcca tttcgagcat aggatccatt ttgccttttt
    18001 tgggtctgaa tacctcaatg cgctcaatgc cctgcactcc catattgaca gtgatatcca
    18061 cagcattggt actccatgga gtaaccattt cacggcgtgg tccgataaaa tcccctttta
    18121 ttccttccgt ggatatttca gcgcagcttc caaaaagcca cctgaatttt tccacatctt
    18181 tttcccccaa ctgagtgcgg ctctgtactg cataaatgag cccttcgctt tcctgtttaa
    18241 aaaatgttaa cattcttccg ggattaattt gcaaacgtaa ggtaaaaaat ggagattaac
    18301 aaatgttaaa attcgtcatg acagaatgct ttgaatatca ctgcaaaagg ccatgaattt
    18361 ataaaacact gataaccggg aaaaatattt tcagtatctt tccatgtttt ttgcaacctt
    18421 ttgaattcca gacatacttt agtacagaat tttgaacaac agcgtggaat ttaccgacca
    18481 gcacagagaa ctgatagaac agtgccgccg tggagagcgc aatgcttttc gcgaagtgta
    18541 ccgcctgtat tcaaaaagca tgttcaatgt agcattacgt atgacaggaa tggcccacat
    18601 agcagaagat attctacagg aagcattcac cgatgcattc agcacgctga acaaattccg
    18661 ccatgaaagc acattcgggg cctggcttaa acggattgtt gttttcaaat gctacgatta
    18721 tctgaattcg aaaagtgccg gatttgaatt tctcgaagac atggaaagct tcgatcaacc
    18781 agaccaggag ccggatttca atgaagaaga aatggcccgc gaagtgaaac gcataaaaga
    18841 agcagttcag ttattgcccg ccggaagccg cacagtgctc acgctctatc ttttcgaagg
    18901 ttatgatcac gaagaaatcg gacagatact caatatttcc gaatccactt caaagacgca
    18961 atacatgcgg gctagactga aactgaaaga attacttacg caatccccct tgcacaatga
    19021 aagataatct tgaaaaattc atatcgcaga accgtcatca attcgatgat gaggagccct
    19081 ctccgggtct gtgggaccgc attgaaaatt cagtgcctgc tccggcacgg atggtcagaa
    19141 tgcggccgct gacctatgct gcttcgattg ccgcagcatg catcattgtc tggattgttg
    19201 cttcagttgt gatgaatcaa cagtcacatg atgctgataa ggttttgtcg gaaaacataa
    19261 ttaggcctga gctgaaggtt gcacaacacg atacggtatt tgtacctgtt gttgtcaatc
    19321 ctgaaaaaaa tacaaccaat accaatgtaa caaacgcttc ggataaaaat ctgtatgcgg
    19381 aaatcacaca atactatgat gccgaaatcg cccaacgaaa agcgaaactg cagtcggtat
    19441 catcgggaaa taaagatata atgtatcagg tgcaggaaga gctggccatg attgatacac
    19501 tcaacgcaca aacacaacgt gaactcggaa acgggatgaa tataggactg gttatggaac
    19561 aactggttca gaattacaga caaagcatcg atatacttga catgatgctg gaacaggtga
    19621 acgaggaata tgcacagata aatgaataag tgtatgaaag caaaactgat tataatgtca
    19681 ttatgtattc tcgctacaga agcggtttgc gcacagtcat tcactgtaaa caaaaagttt
    19741 cacggaagct atccggcagc cgcgaattct gaagtcagta tcgacaacaa atacggcaat
    19801 gtcagcatca ttccatccga tggcgactcc gtggtatttg atgctgttgt tgaagttaca
    19861 gataaataca ttgttgaagc cgaagaacag cttgctgaaa tcaatgtcag aatgggcgct
    19921 tcaggatatt acatttatgc agcaaccgaa ttcaacggca atcgcaatga ttttaaaacc
    19981 gattggaaaa tgatggcagg agccgttttc agcagcaaaa aaaccgtacg gattgattac
    20041 attgtaaaag taccttcagg cgtttcattg aaaatcaaca acagctacgg caacctgatt
    20101 atgccggatc attccggaat tctgaacata gatcttagca gcgggaacct cacagcaggc
    20161 attctgagcg gtaaaacaac aatcacactc aacggcggca aatcgcagat ccgcgcactg
    20221 accgacgcaa ccatcaagac cgacatgaat gaaatgcaaa ttgaaagcgt atcttcggcc
    20281 tttttcgatt cgcgcgggtc acaattcact attcagaaag ccggaaccat aacagtggat
    20341 tcgcgccgcg acaaatggtt tatcggcaac gcaacacaaa tcagcggttc aggcagtttc
    20401 agcaatttca ccatcagccg gctgatgaaa gactgcaaca tgaaaacctt ttacggaaac
    20461 cttacgctat cggccgttga tagtggattt cacagcatca atctgattgc cgggagcagc
    20521 gatgtgagca ttgctctgcc tccgggtgtt gttccgcagt tcaattgtga tcagaaaaaa
    20581 acaagggcca ttctgccggc aaaactgaat ctgaaaaccg aaaccatcga tgaaaaaacg
    20641 cagcaatacc gtacaaccaa tgttgtagaa aatgcaacct cgtttgtcaa tctgaatgta
    20701 agcggcggat cggtgaatgt ttattgaaac atagtgagtg gtgatctgtg agtagttaat
    20761 ggttatttgg ttagccgccc gaaatgcggc cacagatcac accggtgcat attatcccac
    20821 ataagcgcac tgccacaaat gccggtgaag gagccaatcc agagatcgcg gatccaggag
    20881 gcgtcgttgt tttctaatgc attattcatt cttctttttc ctcccaaacc caaactgcac
    20941 gcccgctgta atgctccatc gctcgttgcc gagtggataa taattgtcgg tgaaatcaaa
    21001 cagcaaataa ttattgtcag gaattgaata acacaacatt ttgctaaatt gcgcatagaa
    21061 ctgaactttc ccgaatggtt tggaaatcag cccaacctgc cacgaaggat tgaaattcca
    21121 ttgattgaaa aacccggcac ggataccggc atatattcca tggatcggaa aatcgctctc
    21181 ttctgacaag caacccataa gcgataatga tgcaaaaggt tctgcccgta aataaccata
    21241 agtttgattt tcagaaatat ctttcagtaa aaaattgtgt gttgcaacgc cgactcccac
    21301 attcattttt aagtgcgttg cgttaaaaaa ggaatatgaa atttttcctt cggcattggg
    21361 aagtgcgctg ttgctgatgt tgcgaccgtc ggggtattga attatataca acaatccaaa
    21421 tgaagatgaa agtcgttgtg caaaacaaag ttgcatcaag aaaaaaagca aaacactaaa
    21481 taaaaccttg gttttcatat tattccctta tgtagttata tgaataactc ggaccgtaat
    21541 aagtaccaga tgaaccatca tataaacggt agtaaattcg aagagaatct ttggctggaa
    21601 tgtagattac tgatgtgctt tttttacatc cgatttcatc tttgtactga agataaaatg
    21661 atattgtata ttcacagcca tatgttcctg tattcacttc tacaatagtg taattatcaa
    21721 caatctgaat tattgtattc tctgaataaa atacaattga attatcatca atttttatgg
    21781 actgatcaaa tccaaaagaa tcagggggca aagtatcgga atttgaatat ggataatgcg
    21841 gagtgtaaaa atctgcagaa acaatacaat ttttcagtgt ccaggttcca atcaaatact
    21901 gatactcatc aggcaaaacg ctctctttac tacatgaaag aaagaagagc aatggtgata
    21961 taaaaaatat tatctttttc attggataat taattttacg gtttcaactt tttgattgta
    22021 attcagctgc agaatatata cacctgcatc gcaatctcct acatccagta aaaatgtctg
    22081 accggaaaaa acattggatt tgatcatcat tcccgagcaa gaaaacaaaa tcatcctgca
    22141 gttttccgta ctaactggta aagtaattaa tacggcttga tctgttggat ttggtgatac
    22201 cattgcacgt gacaatgctg taacttttga atttttctct tgaatctcag ctgaaatatc
    22261 ctcgaaatat tttcttgtag aatcagaaaa ttgttccaaa cttttattgt tttttggttc
    22321 cacacatact agtaaaatcg gtctaatcgt tgtgcccgtc aaatatggaa aatcaagtcg
    22381 aagtttacct gtcaaattat acttctcttg agtatgaaca acttgcaaat tatttggatc
    22441 gatataggtt attatatatc tcttctttga tttaacatct acctgcaaag cctcatttat
    22501 attatatgca gtctttgcac tattatatgc ttgatcatcc cactgtatta tatttggcgg
    22561 tgtattcaat tcaaattgcc atcttgttga gtcgctttcc cacacaaacg caccgcattt
    22621 cgccacatcc cattgtgtat aaaaattata agtgcggtta gccaatacac ctactgcatt
    22681 taatttatcg ggtgagcgca gataaaataa atcgacaata ccgggcttgc gctgccagtt
    22741 ttcgtagtgc atttcatcaa aatcatgata aggaatccac cctttctgat caccgttgct
    22801 ttcatcaaaa tcatatcctg ccacaaaagc cctgagccgt ccgaaatgcg gccacaattc
    22861 acgtttgtgc atattatccc acataagcgc actgccgcaa atgccggtga aggagccaat
    22921 ccagaggtcg cggatccagg aggcgtcgtt gtcacactga taatatccct ggtttgttcc
    22981 ggatccgtat tcaccatgaa tgaatggctt atcgcaatat ggccagccat cggaactggc
    23041 attgctatta tcatgcagat cgttcacaat ggtcacagtg ctgttatata tatccggaaa
    23101 acttctgtag ttctgttgag taatgatatc caatgaattc agataatatg tgtgatcgtc
    23161 tgaaatattc ggcccttcgg ttaaataatt cacacttgtc aggtgctgat tatatcccat
    23221 gtatgtagtc atcgaattaa tcatgctgct ttgccatgca tacacttttt gtctgaaaaa
    23281 cgcttcgtta ttataagggg cataaccaga ttccgggaca tccttgcatt tttgttcagt
    23341 agcattataa tacatctctt tagttccgca tgtgccgttt atttcattga tagtttcaaa
    23401 ggcggaaata ttcgtagaat atccccatcg ggcaatcatg tagcgcaacc tgtttgtgta
    23461 atatttaata gcccgataat ccagaaaaaa acttttaggg tctgttaaac caagatcctt
    23521 attgtaacaa taaccatgat ccaagctgtt gtcacattct tccggcaagc ctgcctcgcc
    23581 ataagcagcc cagtcccaaa aaatccggtc cggcttaaga aaataatatt gaattgccgt
    23641 gttgaaatgg ataaacaatc cctcctgctc tgctttttca acaatgttgt caacttccca
    23701 tccgatatgc atccggttgt agtaattgca aatctgttca a
//
LOCUS       DAOPKE010000040        23354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2310_length_23354_cov_10.931628, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000040 DAOPKE010000000
VERSION     DAOPKE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23354
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_23354_cov_10.931628"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKY63_06835"
     CDS             complement(<1..485)
                     /locus_tag="PKY63_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPF00365.1"
                     /translation="MTKENEIKIFEDNKVRALWDSDKEKWYFSIIDVIGILTGSDRPR
                     KYWSDLKSKLQKEGSQLSDFIGQLKMPATDGKFYLTDVADTEQLFRLIQSIPSPKAEP
                     FKLWLAQVAAERLDEMQDPELSIDRALQQYLNLGYSENWINQRLKSIEIRKELTNEWK
                     KR"
     gene            complement(593..1120)
                     /locus_tag="PKY63_06840"
     CDS             complement(593..1120)
                     /locus_tag="PKY63_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPF00366.1"
                     /translation="MKRSHAQAVIPDDLLPAPWLIVRARAKKELFLRNQLLQREFDAW
                     LPEVKRFRIRAGHKEQFMAALIPSYVFVRHLCDQCPDLGFIPGSTGLLMHDGSPALLR
                     HDDALRLQRLCALKAAPELASHYCTGQQVTISSGPLKGFTGIVSRTGSKHFLMVESGI
                     NGIMLKIDMTKNVVE"
     assembly_gap    1786..1885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2007..2080)
                     /locus_tag="PKY63_06845"
     tRNA            complement(2007..2080)
                     /locus_tag="PKY63_06845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2044..2046),aa:Arg,seq:ccg)
     gene            2229..3035
                     /locus_tag="PKY63_06850"
     CDS             2229..3035
                     /locus_tag="PKY63_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HPF00367.1"
                     /translation="MFTRLFANALSLLGFLIGFTSEVLAQYGAPVYFFTVKGSVKDIE
                     CQNPVKNAKVKLIDRNGNAEFDAVTDSLGNFIIFRESEGYYPEYQIRIEDPDGKNNGL
                     FRDIQGPLDAKKFRSTWNHNPDTMPGDIYLVEYISGNPCKNEPVAEEPNPAKEPDPEP
                     EVIAQDSAVLPQEVPIVPPVVADEYFTNINLFPNPNSGHFAVEFTAMDQAATDVYIYS
                     SDGALIYNMSQYAQKGANHFDLELEGLAPGTYYIRLHCFQNDVTKTFVVQ"
     gene            complement(3639..11621)
                     /locus_tag="PKY63_06855"
     CDS             complement(3639..11621)
                     /locus_tag="PKY63_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00368.1"
                     /translation="MAKLLVSLLLMLFITAFVHADEYYWVNGSGYWSDTSHWATTSGG
                     SIYYSDVPGPNDTVFFDAASFSSNGKIITVDMSMANCALMDWSGVAYNVEFNSGTLDT
                     LNVFGHLILSDKVAFNFSGLLRLHGLTNATTYFESQNKSLKCDVELAADTLLMTGALQ
                     LPYNRLLHTDGVFNANGYRVACRHFNTDSSRMAAVPVVAPKWFNTDSLTVSGSFALPN
                     TMFFAQTGPLFFQNSIIDTNYINIYANELICDVYMTGSKKMFLLSQFKTAGVLEFKGG
                     GKFYSQNWPLILGALRNTTSLTKTINLGSSSVLITGAGNALYLNDTRLTFDAALAQFE
                     FNYAGSDTVNIYLGRDSVYTIQDVELPVAVSRIYSSFKTESIFIHPASEVLLAHGIEL
                     VVDTLDANGDCGQYIYLSSFCSENPSGDISTCPSAIPLINAADGEITVSYFKVSYNEL
                     SGAVIHALNSFEFDNVSGWDIVEPAAANVMYWIGGTGHWNDPTHWSNSSGGFSGGCIP
                     VKGNDVVFDQNSFNSDDTVFVDELSFCHLMDWSDIDELAMLSGTGKVFVTGSLLLHDS
                     LDIVLDDGIELNSGTAATKNIRSDHAGIFSEILVNTIGSYHLLDTLQSQSEIILQRGA
                     LSINKQGLICQRIDMSGSEVRSFDYTKSEVVLMGHDTVWNSTGTNLTLVSDSGIVALA
                     WPGTDMVYVKSANSSFDSLMVHSPRTRIYGGGESVLLAVDAGVALEFEPSTIWSFDSL
                     FVNGSCSMPVMLSAFIGETGTATLNKTGYDTLTISDVVIQNLLADSTGGRQYNAVASS
                     AIGVTTGWIFSGSVPGQTYYWTGLIDKNWHIASNWTTDLVTPASCVPGPADTVVFNDV
                     QMNALNDTVVIYRNAFCRVMDWSGIVTRTPALLIEADLQISDDLIMDENLEFGYGGGF
                     SPNDLNPPTLILAPAFSQASFNPSCSRYGVNTVFAGLSLSDTLFMTDTLLTDSTVSVV
                     IKSGSFMSQQFPLTVGILKTETASSKIVDLKNSDISIKYSAVFQDASILALNADSTRI
                     VMPGNKSFISYFDGGNQRFFDFEMITLYSDSAGIFYNGQVFGADTFRIFTVHPDMHLY
                     FEAGMEQTFDSAFIASGTCQELIYIASTEEGSAAILTSLASDSIRTQCTVVRDITSSN
                     GAVAVFSTDVSNNTNWRFDAVPATTASFTVSGPTCYGDSVHFVNTSTAYSGNQGDLTF
                     EWYMQDTSLTTTVVSPVHYFSNNQKYGVTLVSTFSNGCTDSFTDSVTIYKPVINLSSS
                     DADTTICDGSLVTMTANSSNPSYHFYFFSDGIPVIQSPDSTKYRTDSISDGETIFAVV
                     DYMGCFDTSGVFTFSVDTLPLVSLNSSDADQIICLGDSVTFLASGADQFQLYLNGVAY
                     GNLGVNAAWITDTINTADEFTLYGRNVSTGCESYSADSLLFTVLPLPVVSLTSSEADT
                     TICAGDTVVIHAAGANEYVFYLNGVPISPQSTVDSVVVSSLADNDIVSVEGISLAGCR
                     NYAPGSLVFHVEVTPLLSFVSDDSDSRICDGQQITFQAGGATEYEFFIDGVSTGPLST
                     NNSFTSSFSNGQSVSVQGRLGNCYSFADSIFTIDVRPTITWSYSDSVICANESINLEA
                     EGDTVYQYFIDFVPVTPLQYDSLYLASGLADGQVITVSGTEYACTPAPLTVTVYPVPL
                     VNVACSDPDTAICDGSVVSFTASGSETYGFYVNGVATGPYSTVNVYSVDTLNNGDMIS
                     VQAYSANGCLSVAEDTFIVEVAPNPVVVVTQSDPDLTICAGDTVYFNASGADSYEFFV
                     AGTSQGSPSAVSSIALGNLFNGNEVTVNGTSGYCTSTSSDVYQYTVNPIPTITFSPVS
                     GTSVCSGDTIQILAGGAMSYQFYVDALPWGAESGNSLFQCDTLTTGQIVSVEGTLAGC
                     TGTSSQTYSVTVNDYPSLSFINDIPSGYICFGDTVTFEGNGAQHYTFYLEGMPVSTDS
                     VFVSGDIQNGQEVTLYGYNGVCGLWADTTMTVYVNYVDVDLSSDMPSTALCAGSAVQF
                     TASGADMYEFFIDGLSQGSPSATNVFTPSVINDGQVVSVVGTSSLSGCAQSAFNDVYV
                     HIIELPLISASPDVVFCEGDSVLLISSLNQNNHWFFEGTPVAAVTDSTIWAYEGGNYY
                     SVVSAGSMGSVWSCGENSLGQFGDGTTFNSIDMQQSVLSTYALEVACGAEFTLVLNDD
                     GSVKAWGRNEFGALGNGNYTDSNLPVSVGSLTDIHKIAAGNRFALALKSDGTVVSWGE
                     NTYGQLGYGNYSTSNFPFAVLNIDSVTDIAAGENHALALTENGHVWAWGLNQAGQLGD
                     STQINRNSPVMVKHLDDVVAVAAGANHSMALTADGSLWLWGNNASGQLGNGSYNSSWI
                     PVKVNLYNGIVNFDGGFAHSIACDTMGMVYTWGDNTYGQLGLSMLAESLFPVHSDVSG
                     GVVDVKAGKYSSFALRVDSNLVSWGQNQAGQLGYSGAASVFSPQVIDQTFGVGSFDAG
                     DRHLAVIPVHDQSCSASGITLSMDTVPDIDIVLNGLILSTTASGVLYQWYYNGSAIPG
                     ATGTSVTIAAFGTYYVVVTFANGCSGFSEEFDYGVGMDEYGVSQLLLYPNPSNGEFTV
                     MLSSNDQARSWRILNSLGQVVAAQNELNNAVFEVRGVALSPGVYSIQVICGDGAVRSA
                     KFTVTE"
     gene            11769..12296
                     /locus_tag="PKY63_06860"
     CDS             11769..12296
                     /locus_tag="PKY63_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00369.1"
                     /translation="MKSFILYISLFLIVINTGFGQNYTDSLKSKLYIEKDLDTLYSEI
                     VNTGAKLRLPMYFAPFSQDKHHGYVHVGTATTIMGQKMDSTAFLSVTTNLNQEVFLKQ
                     GAELIEELDMKTLEGRPAKMYLIRFTADKTKINRIMFFTGDLNSTLLLTANYPEIFSE
                     LLKDVILSSFITVQY"
     gene            12309..16112
                     /locus_tag="PKY63_06865"
     CDS             12309..16112
                     /locus_tag="PKY63_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00370.1"
                     /translation="MKRKILLICLIVTSVLPSMAQTTTVTQYANFYTNDQSLWMQGNS
                     GLLDLDIPFFDVSWNENGSIGGITTVAGYPFGAVVNAGTWGQIGSGLRIEFGTEKVDV
                     VYNTDVNLTMPADGTFEKGDEITISTALDPYENTSSITRDEYNVIFQIWMAIGFGFDI
                     GGQFCFFDCTNGTIVDIDLPVDTFNLVYLDSTGVSLLEGLWEWPFSNLPISFSDNKDI
                     VTLTLDLPSNANDSSWFSGNNMISASRPYEYASLEFNVCKFIGALNIPYVSAVFANLE
                     NEWELGPVTIGYTLMDMVFKLGLYHNQRLTFSPTVKTTLTFPGNIDYKVLKPDNNVLS
                     SGTASSITLDVGNKLRFRYPCNYDFMDIDPYYVMRNNFNNHTYDSIAFDFIFDMLEFH
                     VSMDDIEVIPEICIPIYYPCGPWYCVVCDWCYDGDICTPAVVFHGFNWGFGPLVHLQP
                     NIFNVKYNWVNSNWEMQDFNDVDGDIFRITPARFEVSVAPSDAPCFGINGGSAIATVT
                     HGKPPYRYEWSNGTVHTNVVSLTDAVSNLPAGTHYVIVTDINGCVTFCSFVIGQPEAA
                     LTIDAISTNISCNGTPSGSINISTSGGTTPYSFNWSNGATTEDITNVTAGTYILTVTD
                     SLGCTNVSSYTLTEPYPLISTASSTNPRCYGAASGFAAVSSTGGTPPYNYLWNTGDTT
                     QSISNITAGAYSVTVTDFNGCENILTYAITQPADPVSIIISTQDVFCNGESSGSVTVS
                     ASGGTAPYQYDWFRNGQSLNHTAANLGNIDVGDYRVIVTDANGCTKDTTVHISEPPAI
                     EFNVAVVNNLCFGETNGSISLSVNGGTGTYTYNWSNGASTQNIGTLPAGTYQVTITDN
                     NGCHQYAEAIITQPGAPLTATAEPTHVLCKGNNTGVATLNPQGGTAPYFFLWSNGSTN
                     QNPTGLTAGTYTVTITDNNGCIAYSGTVIEEPGDSLAATFNISDALCFGSSDGNITAM
                     ASGGTTPYYIRWDDTDYLMQNNGHKIENLYSGYYQIIVTDANGCKSAQTLFVNQPDSI
                     HSSAIEGITSCWGGNDGIIDLSASGGTAPYGFIWSNGQTSEDVSSAETGTYSVTITDD
                     HGCTKTATYHIGTMPEIIVNASVVQVSCSDNADGQITLIVSGGAGSYNYLWSNEATSY
                     QLSNLEPGSYSVTVSDLNGCTKIIEYTLPESETECINIPTSFTPNSDGKNDDWVINNI
                     DLYPGHRVQIFNRWGNLLYEQSPYTTPWDGKFNGNPLPAETYYYIIDLNNGQEAFTGT
                     VTIIR"
     gene            16122..18560
                     /locus_tag="PKY63_06870"
     CDS             16122..18560
                     /locus_tag="PKY63_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00371.1"
                     /translation="MKLILSISVFLWLTTAFADNGDPVKNEGNSPAPCFSVSLTGDPN
                     PCYGDSLGTVHLTINGGTGPFTISWSSGETGVYDISGKPAGYYDVQVIDQGTGCVAFD
                     IFNVIEPTQLTSSLTQTDVKCFGQSTGSINLTVAGGKPGYTFLWNDGASTQNRINLDS
                     GLYIVYITDANLCRDTIETIIQQPAQALSRSLTYENPLCFGDFNGSVDLTVWGGTTPY
                     IYDWNTGTFASQDLSGINAGTYNVVITDANNCISNASVTLTNPPQLTLAVTSTDNNCF
                     GELDGTVDVTVGGGTLPYHYTWANETFVLSWTDEDLDSLPNDTYYVTVTDANGCQLNN
                     SATISSPDQLTISLSSSNVTAFGGSNGSVSSTVGGGIVPYIYSWSNGATTPDLNNIPA
                     GWYILNITDSHGCSITDSVYISEPSDPLTVSLSGTLITCFGGSDGISEAIGSGGTAPY
                     TFLWSTGSTETRVTGLQAGFYSITITDYYGNTASDTIEILQPDNFVFTQTITDVTCFG
                     LGNGSIDVSISGGTAPYTYEWLNSQYVLAAITEDISGMPADQYMLQLTDTFGCTGSHT
                     VMISQPDLLVADISYTDAYCAFSSTGTASADVSGGTAPYIYLWSNSETMADISALTAG
                     TYILTVTDNNNCFFTDSVIISEPDSIAITYATTPVSCRDQHDGTISTYASGGNGNYEY
                     LWNTGDMTASLSDLLAGIYSLTVTDMMGCTGHADIEVTRIEIDCINIPTSFTPNADGI
                     NDSWVINNSELYADATYQIFNRWGQEVYSVKGIWAPWDGTWNGNPIPAETYYYFIRLT
                     PDSQTLQGTITIVR"
     gene            18574..19509
                     /locus_tag="PKY63_06875"
     CDS             18574..19509
                     /locus_tag="PKY63_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPF00372.1"
                     /translation="MKKIFLPLILMLFAQATLAQQLSLNSQYMMNRFVLNPAIAGNYN
                     HIPINFNIRKQWDGINGAPVTQSLSAHSYLGSNVGLGGYFFNDVTGPTRRTGMNVSVS
                     YQLKLNSDFSRKLSFGLSGVFFQHFVDKDKLTTNEPDDIAITNGFNNQFCPDANFGVY
                     LSQTNKYYLGFSIQNLLEVKKDLFDMNRLVDNPVERTYYANGGYTFKLNDDFVFEPSF
                     LLQFQETTPIQMDFNGRFGYKNKFWLGGSYRMNDAAVVMVGLDFDDFCIGYSYDITMS
                     DIKNYSSGSHEINLTYRIYKKGALRQNCKSTPLFN"
     gene            19525..21354
                     /locus_tag="PKY63_06880"
     CDS             19525..21354
                     /locus_tag="PKY63_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPF00373.1"
                     /translation="MTRLFFSSVLILLMSARLSAQSANASELMNKGCYAEASVLFEKT
                     VTGNNPEYSDLTNLAYCYIMMHDFVKAEAVYERILQHPKKENIQHFYYGEVLRINGKY
                     DKAKEQYDIYKTAFPDDFKADQRIRSCDSIALWNKNKTKYSTGKIFDLNTDNDEMFPW
                     MADGVLHFSSNSRFLLEKNGATSNYKDPQIFNIFVYKNNSVALKKIAAKDSSSFTAYV
                     PAYGKELLLTKKIKVTPTGEELSPSEISVDKKTFIPSGAPQGYTFSHPCLAGNGKRMY
                     FVSDMPGGFGGTDIYYSDFDGTSWSTAVNAGNNINTPGDEMFPYITAHDSLLYFSSDG
                     HPGYGNMDIFVSRISSGKWSSPVNMKAPVNSIGNDFSMVYKNYPWDGYLVSNRYPDSK
                     GGNDIFSFSFEKPTIIKPVDTTKPFVYNADPSLLYAFFETGSSEIAPEYKSIMDSLIG
                     LMKKYDYLNLSVTSFADVRGTEKLNAQLVQERMNAVSGYFSSAGISSSRIIGTPGKTA
                     SDREIPNLTFHVQIGFVAKENQEAYFEWRTYNKYKVSSFKKKNGYTYFTGNGSLNDMQ
                     ALARDINTLFNLGAYVIVSYRGIVFDDTFYAPNRRVEMKLFNK"
     gene            complement(21358..22101)
                     /locus_tag="PKY63_06885"
     CDS             complement(21358..22101)
                     /locus_tag="PKY63_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPF00374.1"
                     /translation="MKLSVIIIDDEKPARDIIASYAAQRADIEMAGECQNGFEAIKMI
                     NELRPDLIFLDIQMPKVDGFELLEVLEYRPAVIFCTAYDHFAVKAFEKSAVDYLLKPF
                     SEARFNEAVDKLILQKKQSGSAQDKVISETESLREEAGEKVSRVVARLGSNLHVIQVS
                     DIRFIESQDDYVLIHCSKGDFLKEKTMNYFEKNLPEEFLRIHRSYIVHIQCIDKVELY
                     EKDTHLVTLKTGEKLRASREGYKKLRALL"
     gene            complement(22098..23135)
                     /locus_tag="PKY63_06890"
     CDS             complement(22098..23135)
                     /locus_tag="PKY63_06890"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF00375.1"
                     /translation="MKSRSHIFFTYFSAWILITVFYAVLLWMAYNIDFRWAAADSVIS
                     TTLLFINGLALYFMLQYEDHKRFTRARSIFQMVFGYLVAVSLFYIVSDPLLQYISESN
                     EAYKSLYELSRIPRMIVAAMLLLLMLLAFRLYKNLQMLREKTEQGMRLQSLLQESELN
                     ALRWQINPHFLFNSLNSVSSLTIANPQQAREMIIKLSELLRYSLRKNDEQFSTLEQEI
                     NHIRIYTDIEKVRFGERLNVAIHAPDKCLSMKMPFLILQPLIENAVKHGVYSTEQQST
                     IQVICECSDPFLVITIENSYDPEGRTRTGTGTGLSNTAGRIRLMYNDDSLFRTSDSGN
                     VYKVTLKIPKR"
     gene            complement(23136..>23354)
                     /locus_tag="PKY63_06895"
     CDS             complement(23136..>23354)
                     /locus_tag="PKY63_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00376.1"
                     /translation="LAEGDNILEISAVFGGFKLIVPAHWNVKIETSNVFGGVVDKRIV
                     TGTVDMTRTLVVRGSAVFGGGEITSILD"
BASE COUNT         7095 a         5429 c         4762 g         5968 t
ORIGIN      
        1 cgttttttcc attcattggt gagttccttt cgtatctcaa tgcttttaag cctttgattg
       61 atccagtttt cggaatagcc cagattcaga tactgctgca atgccctgtc aatactgagc
      121 tccgggtctt gcatttcgtc cagtcgttct gctgctacct gagccagcca gagcttgaag
      181 ggttcggcct tcggcgaagg aatagattgt atcaatctga aaagctgttc cgtatcggcc
      241 acatcggtta aataaaattt gccatcagtt gcgggcattt tcagttgtcc gataaaatcg
      301 gacaactgac ttccttcctt ttgtagtttt gatttcaggt cgctccagta ttttctaggt
      361 ctatcagagc cagttaaaat tccaattaca tcaataatag aaaaatacca tttctcctta
      421 tctgaatccc atagagctct tactttattg tcttcaaaaa tttttatttc gttttctttt
      481 gtcataccgg ggctgtttaa aattatcgat tattaatgtc cgtttgtttt tgtcggcttc
      541 tgtaccactg ttattcagag tcaatcattt taatgcagat atcctgcatc cctcactcca
      601 ccacattctt cgtcatgtca attttaagca tgatgccgtt gatgccggat tcgaccatga
      661 gaaaatgttt gctgccggtg cgggatacta tgccggtaaa gcctttgagc gggccggaac
      721 tgatggtgac ctgctgaccg gtgcaataat ggctggcgag ttcgggagcg gctttgagcg
      781 cgcacaggcg ttgcaggcgg agcgcatcat cgtggcggag caaggccgga gagccatcgt
      841 gcatgagcaa accggtgctg cccggaataa aaccgaggtc gggacactga tcgcacaagt
      901 ggcgcacaaa cacatagctg ggaatgagtg cggccataaa ctgttcttta tgtccggcgc
      961 ggattctgaa ccgtttcact tcgggcagcc atgcatcgaa ttctcgctgc aggagctggt
     1021 ttcggagaaa caattctttt ttggcacgtg cacgtacaat gagccaggga gcaggcagca
     1081 aatcgtcggg aatgacggcc tgcgcatggc tccgcttcat cctggttttg cttgaagcag
     1141 gatcagcagc gggaatatgg gtgttctcgt gagggaacat gacggtgtaa atataggaat
     1201 ttttgaaata gtttagaaaa gattttttac tttattattc gttacaatcg ttacagtcgt
     1261 tacacgttac agacgttaca cggcaggcgg caggcgcaac gcacaatgcg caacgcataa
     1321 cgcacaatgc gcaacgcaca gggcacaatg catgcggcag gcggcataag gcgggcggct
     1381 tgtggtagct tcgcactgaa cgctgcggcg ggcaaaatac aaaatgagaa atacaaaaca
     1441 cgaaatgcga aatacaaaat atgaaatatg gaagttataa tctgaaagcg atcggctgac
     1501 aaactgtaaa gcatcatctt caacggtcgc tggtcgcttc acataaaacg caacacagtc
     1561 gcgacgcaga aaagttgaag atgaattcgt ttagaaacta aaaagaaact ccgaagggac
     1621 ttccgccagc gggcggataa acttctcgtc cctttcggag aagtctgaaa atacaaaaag
     1681 ctaagagtga ctccgaaggg acttccgcca gcgggcggat aaacttctcg tcccttcgga
     1741 gaagttttga tgtataagaa gctgaagtgt gactccgaag ggactnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnntccct tcggagaagt tttgatgtat aagaagctga
     1921 agtgtgactc cgaagggact tccgccagcg ggcggataaa cttctcgtcc cttcggagaa
     1981 gttttgatgt ataagaagct gaagtgtgac tccgaaggga ctcgaacccc caaccaacag
     2041 aaccggaatc tgccattcta tccaattgaa ctacggagcc gtaaagcggt gcaaagatac
     2101 gaattttaag attacaagaa actaaggcca attcaatgac ggcgaagtat aataaatata
     2161 cggaatttaa cgttttacgg aacaattatt ggttcaaatg cgccctgatt ttttaaaaac
     2221 actgcgttat gtttacacga ttatttgcca atgcattgtc tttgctgggt tttttaattg
     2281 gcttcacttc cgaagtgctt gcccagtacg gagctccggt gtatttcttc actgtaaaag
     2341 gttctgtaaa agacattgaa tgccagaatc cggtgaaaaa tgcgaaggta aaactgattg
     2401 accgcaatgg caatgctgaa tttgatgcag taactgatag tcttggcaac ttcatcattt
     2461 ttcgggaatc agaaggatat taccccgaat accagatcag aattgaagat cccgacggca
     2521 aaaacaacgg cttgtttaga gatattcaag gtcctttgga tgcaaagaaa ttcagatcga
     2581 catggaatca caaccccgac accatgccgg gcgacattta tctggtagaa tacatttcgg
     2641 gcaacccttg taaaaatgag cccgtcgctg aagaacccaa tccggccaaa gaaccggacc
     2701 ccgaaccgga agtcattgca caggacagcg ctgttttgcc gcaggaagtt cccattgttc
     2761 cgcctgttgt tgccgatgaa tatttcacca atataaatct gtttcccaat ccgaattcgg
     2821 gacattttgc cgttgagttc accgccatgg atcaggcagc aaccgatgtt tacatttact
     2881 cttcggatgg cgcgctgatt tacaacatga gtcagtatgc acaaaaaggc gccaatcatt
     2941 tcgatctgga acttgaaggc ctggctccgg gaacgtatta catcagactc cactgttttc
     3001 agaatgatgt aacgaagact tttgtggtgc agtagcacat gcggcttgcg gcatagggca
     3061 tgcggcttgc ggaatgagat tcagacgcgg aataacgctg attaactgat atcacaccag
     3121 ataaacgcgg aaaaaagatt gacacaacgc taataccagg atgaatatta aaacaggccg
     3181 attcagccgg ccttgcggtt tcttttgaca ttacattcac gcctatgagt gataaacttc
     3241 ctgattaaag gaagacaaac ctgttccgcc aaaggtggaa gcggaatgtt gtggcagctg
     3301 cattcaacaa ctgtcccgtt agggacagga tgttggtaga aaaaagaaaa atgaatccgt
     3361 cccgttaggg acgaaacggt cgccaataaa ccattatgta cctgacggca cgagcccgat
     3421 tctattatat tagctaccaa tattttgtcc cttacgggac ataaaaacag aaacaaccct
     3481 gatccgccaa agtcggaagc ggaatgttgc gatgcgttgt aagagctttt gaattacaga
     3541 acaattcatt tttgtacaac acatttaaag aaaaaagagc ttaaagaaaa tcaatcttta
     3601 agctcttttg atttctgata aaactattca gacggcaatt attctgtcac agtgaattta
     3661 gctgatctca ctgcgccatc gccgcaaatg acctgaatgc tgtaaacgcc cggtgagagc
     3721 gcaactccgc gtacttcaaa cacagcatta ttcaattcat tctgcgcagc aacaacctgt
     3781 ccgagagaat tcaatatcct ccacgaccgc gcctgatcgt ttgaagaaag cattacggta
     3841 aattcaccat tggaaggatt tggatacaac agcaattgcg atacgccata ttcatccatg
     3901 ccgacaccat aatcaaactc ttcggagaag ccggagcatc cattggcaaa agtaacaaca
     3961 acataatatg ttccgaatgc tgcgatggtt actgatgttc cggtagcacc cggaatcgcg
     4021 ctgccattgt agtaccactg atataaaaca ccacttgcag tggttgataa tattaaccca
     4081 ttcagaacaa tatcaatatc cggaactgta tccatactaa gcgtaatccc agatgcagag
     4141 caagactgat catgcaccgg aattacggcc aaatgcctgt cacccgcatc aaatgaacca
     4201 acaccaaaag tctgatctat cacctgcggc gaaaaaacac tagctgcacc actgtatcca
     4261 agctgtcctg cctgattctg gccccagctt acaagattac tgtcaaccct caatgcaaaa
     4321 gaagaatact tgcccgcctt cacatcaaca acacccccgc tgacatcgga atgcaccggg
     4381 aaaagtgatt cagccaacat tgacaatcca agctgaccat aagtattatc gccccaggta
     4441 taaaccattc ccattgtatc gcaggcgatt gaatgagcga aaccaccgtc gaaattcact
     4501 attccgttgt acagatttac ttttaccgga atccatgaag aattataact tccattaccg
     4561 agctgcccgc tggcattgtt tccccacaac cagagagagc catcagccgt aagagccata
     4621 ctgtgattgg ctcctgcagc aacagcaaca acatcgtcga gatgtttcac cataaccggg
     4681 ctattgcgat tgatttgcgt actatcgcca agctgtcccg cctgattgag accccaggcc
     4741 cagacatgac cattttcagt cagcgccagt gcatgatttt cgccggcagc aatatctgta
     4801 acactatcaa tattcaaaac tgcaaacggg aaattgctgg tggaataatt tccatagccc
     4861 aattgaccat aggtgttttc gccccatgaa accactgttc catcgctttt taaagccaat
     4921 gcgaaacgat ttcctgccgc gattttatga atgtcggtca agcttcctac agagacaggc
     4981 aaatttgaat cggtataatt cccgtttcca agtgcgccaa attcatttcg gccccacgct
     5041 ttaacagagc catcatcatt gagcaccagt gtaaattcag caccacaagc cacttctaaa
     5101 gcatacgttg ataaaacaga ctgttgcata tcaatgctat taaaagtcgt tccatcgcca
     5161 aactgcccca aactattttc accacacgac caaacactac ccatgcttcc agctgaaaca
     5221 accgaataat aattaccacc ttcgtacgcc cagatggttg aatcggtgac agctgcaaca
     5281 ggtgttcctt cgaagaacca gtgattgttt tgattgagac tgctgataag cagtactgaa
     5341 tcgccttcac agaaaacgac atcgggcgaa gccgaaatta gcggtaattc aataatatga
     5401 acataaacat cgttaaacgc agattgagca caacctgaaa gactggatgt tccgaccacc
     5461 gaaaccacct gtccatcatt gataacagaa ggggtgaata cattggtcgc agaagggcta
     5521 ccctgcgaga gaccatctat aaagaactca tacatatcgg ctccggaagc agtaaactgt
     5581 actgcagaac ctgcacacaa agccgtcgaa ggcatatcgg acgacaaatc cacgtcaacg
     5641 taatttacat agaccgtcat ggtggtatcg gcccacagac cgcatacacc attgtatccg
     5701 tacaaagtta cttcctgtcc gttctgaata tcaccggaaa caaaaacgga gtcggttgag
     5761 acgggcatcc cttccagata aaaagtataa tgttgtgcgc cattcccttc aaaagtaaca
     5821 gtgtcgccaa aacagatata accggatggg atatcattga taaaagaaag cgacggataa
     5881 tcattcactg taacagaata agtctgcgac gaagttccgg tgcatcctgc caatgttcct
     5941 tccacagaaa caatctgacc agttgttagc gtatcgcatt gaaacaatga attgccggat
     6001 tcagcacccc atggcaaggc atcaacataa aattgatacg acattgcacc accagcaaga
     6061 atctgaatgg tatcgccgga gcagaccgaa gttcctgaaa ccgggctaaa agtaatggtt
     6121 ggaatcggat tgactgtata ttggtacaca tcagaagacg tagaagtaca atagccactg
     6181 gtaccattaa cagtaacctc atttccattg aacagatttc caagggcaat agaagaaacc
     6241 gcagaaggag atccctgtga agtgccggca acaaaaaact catagctatc agctccggaa
     6301 gcattgaaat aaacagtatc tccggcacaa atagtcaggt ctggatctga ttgtgttact
     6361 acaacaacag gattgggagc cacttcaaca ataaaagtat cttcggctac agaaaggcat
     6421 ccattggctg aatacgcctg aacactaatc atatcgccat tattcaacgt gtcaaccgaa
     6481 taaacattaa cagtagaata aggccctgtt gcaacaccat ttacataaaa accatacgtt
     6541 tcggaacccg aagcagtaaa gctaactaca gagccatcac aaatagctgt atcgggatct
     6601 gaacaggcaa cattaaccaa aggaaccgga taaacagtaa cagtcaatgg cgccggagta
     6661 catgcatact ctgttccact aacagtgata acctgaccat ccgccagccc agaagccaga
     6721 tacagcgaat cgtactgcaa tggcgttaca ggaacaaaat caataaaata ctgatatacc
     6781 gtgtcgcctt ctgcttccag attaatagat tcattagcac aaataacact gtcggaatat
     6841 gaccaggtga ttgtcgggcg cacatcgatg gtgaaaatgg aatcagcaaa ggaataacaa
     6901 ttcccgaggc ggccctgcac tgacacggac tggccattgg aaaatgatga agtaaaagaa
     6961 ttatttgtgc tcaaaggacc ggttgataca ccatcaataa aaaactcata ctcggtagca
     7021 ccaccggcct gaaaagtaat ctgctgaccg tcacagatac gactgtcgct atcatcactt
     7081 acaaaagaaa gcaaaggagt aacttcaaca tgaaacacga gtgatcccgg tgcataattt
     7141 ctgcaaccgg caagagaaat gccctcaaca ctaacaatat cattatcggc aagggatgaa
     7201 acaacaacac tgtcaaccgt tgactggggg cttattggca caccattaag ataaaaaaca
     7261 tattcatttg caccggcagc atgaatgaca actgtatcac ctgcacaaat ggtcgtatcg
     7321 gcttcggatg aagtgagaga aacaaccggc agcggaagaa cagtgaacaa caaagaatct
     7381 gcactataag attcacagcc ggttgagacg tttcttccat acagagtgaa ttcatcggcc
     7441 gtatttattg tatctgttat ccaagcagca tttacaccaa gattgccata agcaacacca
     7501 ttcagatata gctgaaactg atcagcacca gacgcaagaa aagtcacact gtcgcccaaa
     7561 caaatgatct gatcagcatc gctgctgttt aatgagacaa gtggcaatgt atctactgaa
     7621 aaagtaaaaa cgccactggt atcgaaacaa cccatgtaat caaccactgc aaaaatagtt
     7681 tctccatcac tgatggaatc agttctgtat ttagttgaat caggcgattg aatcacagga
     7741 atgccatccg aaaagaaata aaaatgatag gacggattag atgaattggc ggtcattgta
     7801 acaagtgaac catcgcagat tgttgtatcc gcatcgctcg aactcagatt aattacaggt
     7861 ttgtaaatgg tgaccgaatc agtaaatgaa tctgtgcaac cattgctgaa agtagaaaca
     7921 agcgtaacac catacttctg gttattgcta aagtaatgaa caggagaaac aactgttgta
     7981 gtcaatgaag tatcctgcat ataccattca aaagtgagat caccctgatt accgctatat
     8041 gctgttgatg tattcacaaa gtgaacactg tcgccatagc atgttggacc cgaaacagta
     8101 aacgaagctg ttgtggcggg taccgcatcg aagcgccagt tggtgttgtt tgaaacatct
     8161 gtgctgaaaa cagcaacagc tccattgctt gaggttatat cgcgcacaac ggtgcactgt
     8221 gttcggatac tgtcgctggc aagactggtc agtattgccg ccgaaccttc ttcggtagaa
     8281 gcgatgtaaa taagctcctg gcatgtcccg gaagcgataa aggcactgtc aaaagtctgt
     8341 tccattcccg cttcgaaata aagatgcata tcaggatgca ctgtaaaaat gcgaaaagta
     8401 tcggctccga agacctgacc attataaaaa attccggctg aatcagaata caatgttatc
     8461 atttcaaaat caaaaaaccg ctgatttccg ccatcaaaat agctgataaa cgacttgttg
     8521 cccggcataa caattcgcgt agaatcggca ttcagagcca atattgaagc atcctgaaac
     8581 acagctgaat attttattga aatatcagaa tttttcaaat caacaatttt agacgaagct
     8641 gtttctgttt tcagaatacc cactgtcagc ggaaattgct gcgacataaa agacccggat
     8701 ttaatcacaa cagaaaccgt tgaatcggtt aaaagagtat ccgtcataaa taatgtatcg
     8761 gacagtgaca atcctgcaaa cacagtattt acaccatatc ggctgcaaga cggattaaag
     8821 ctggcctgcg aaaaagcagg cgcaagaatc aacgttggcg gattcaaatc gttaggagaa
     8881 aaaccaccac catagccaaa ttcaagattt tcatccatta tcagatcatc cgaaatctgc
     8941 aaatctgctt cgataagcaa agccggagtt cttgttacaa tgccactcca atccattaca
     9001 cgacagaaag cattgcgata aatgacaacg gtatcattca atgcattcat ctgaacatcg
     9061 ttgaaaacca cagtatctgc tggtcccgga acacaggatg cgggagtcac aagatctgta
     9121 gtccaattgg atgcaatatg ccagttttta tcaatcaaac ctgtccaata atacgtttga
     9181 ccgggaacag agccggaaaa tatccatccg gtagttacac caattgcaga agaagcgacg
     9241 gcattgtatt gacggccccc ggtagaatct gccaacaggt tttgtataac tacatccgaa
     9301 atcgtcaaag tatcatatcc tgttttgttc agtgtcgcag tgcctgtttc accaatgaaa
     9361 gccgatagca taacaggcat tgaacaggaa ccgtttacaa acaaactatc aaaagaccag
     9421 attgtggatg gttcaaattc gagcgcaaca ccagcatcga ctgcaagcag aaccgattct
     9481 ccaccgccat aaatgcgggt tctgggagaa tgaaccatta gcgaatcaaa tgagctgtta
     9541 gctgatttta catacaccat atcggtaccc ggccatgcaa gagcaacaat accggaatcg
     9601 gaaaccaacg tcaggtttgt acctgtagaa ttccaaaccg tatcatgccc catcagaaca
     9661 acttctgatt ttgtataatc gaaactgcgg acttcgctgc cagacatatc aatacgttga
     9721 caaatcaacc cttgtttgtt tattgataaa gctccacgct ggagaataat ctctgattgt
     9781 gattgcaacg tatcaagcaa atggtaggat ccgatggtat ttacaagaat ttccgaaaaa
     9841 ataccggcat gatcggatct gatatttttt gttgcggccg tacccgaatt caactcaatt
     9901 ccatcatcaa gaactatgtc caaactatca tgcagcaaaa gagatcctgt tacaaaaacc
     9961 ttacctgtac cagaaagcat agcgagttca tcaatgtcag accagtccat caaatggcaa
    10021 aatgaaagtt catcaacaaa aacagtatca tccgagttaa atgaattttg atcgaagaca
    10081 acatcattgc cctttacagg aatacaacca cccgaaaaac cacccgacga attcgaccaa
    10141 tgcgtaggat cgttccaatg gcctgtacca ccaatccaat acataacatt tgcggcagca
    10201 ggttcaacga tatcccatcc cgatacatta tcaaactcga atgaattcaa ggcatgaata
    10261 acagcgcccg acaactcatt gtacgaaacc ttgaagtatg aaacggtaat ttcaccatct
    10321 gctgcattaa taagagggat agcagaagga caagtggata tatctccgga tggattttcc
    10381 gaacaaaaag atgacagata aatatattgt ccgcaatcac cattggcatc cagagtgtca
    10441 acgacaagct ctataccatg cgccaaaagc acttccgagg ccggatgaat aaatatgctc
    10501 tctgttttaa aagaagaata aatacgagaa accgcaacgg gtaattcgac atcctgtatg
    10561 gtataaaccg aatcgcgtcc cagataaata ttcactgtat cagaaccggc ataattaaac
    10621 tcaaactgag ccaatgcggc atcaaaagtc aaacgcgtgt cattcaggta caaagcattt
    10681 cctgccccgg taattaatac tgaagaggaa ccaagattga ttgtttttgt tagagatgtt
    10741 gtattgcgca aagcgcctaa tatcaatggc cagttctgag aataaaattt tccaccgccc
    10801 ttgaattcaa ggactccggc tgttttaaac tgactcaaca aaaacatttt tttgctccca
    10861 gtcatataaa catcgcaaat caattcattg gcatatatat taatgtaatt ggtgtcaatg
    10921 atggaattct gaaaaaacaa aggaccggtc tgcgcaaaaa acatagtatt ggggagagcg
    10981 aaagaaccac ttacagtcag agaatccgta ttaaaccact tgggagcgac cacgggcacg
    11041 gcggccattc tggaagaatc ggtgttgaaa tgacggcaag caacccgata accatttgca
    11101 ttaaagaccc catcggtatg aagcaaccgg ttataaggca actgaagggc tcccgtcatc
    11161 aataaagtat cggcagcgag ttcaacatcg catttcagtg atttattctg tgattcaaaa
    11221 taagtagttg catttgtaag tccatgcaat ctcagcaaac ccgagaaatt gaacgccacc
    11281 ttatcggata aaatcaaatg accaaaaaca ttcaatgtat cgagtgttcc tgaattgaat
    11341 tcaacattat aggcaacccc tgaccaatcc atcaatgcac aattggccat agacatatca
    11401 acggttatta ttttcccgtt gctgctaaaa gacgctgcat cgaaaaacac agtgtcgttg
    11461 ggtcccggaa catcggaata atagatcgaa ccgcctgatg tagtagccca atgactggta
    11521 tcggaccaat atccgctacc gttcacccaa tagtattcat cagcatgcac aaaagctgtt
    11581 ataaacagca ttaaaagtaa agaaaccagc agtttagcca taaacctgtt tgttaatgga
    11641 agattaattc aagcgactaa aataaggaat attattatta gtcgacaaaa aataaatatt
    11701 ttttttatgt ttttgtacta gatatataaa tttgcagtat cttgcactct aaattgaaat
    11761 ttcagccgat gaaatctttt atcttgtata tcagcctgtt cctgatagtg attaacactg
    11821 gttttggaca gaattacaca gactcgctga agtcgaagct ttatattgaa aaagatctgg
    11881 acacattgta ttcagaaatt gtcaacacgg gagcaaaact tcgcctgcct atgtattttg
    11941 caccattttc acaggataag catcatggct atgttcatgt aggcacagcc acgaccatca
    12001 tgggacaaaa aatggattca acggcatttt tatcggttac caccaatctg aatcaggaag
    12061 tgtttttaaa gcaaggagcc gaattgattg aagagctcga catgaagaca cttgaagggc
    12121 gaccagcaaa aatgtattta atacggttta ctgcagacaa aacaaaaatc aaccggatta
    12181 tgttttttac aggcgactta aacagcacat tactacttac cgccaactac cctgaaatat
    12241 tttcagaact actgaaagat gttattcttt caagttttat tactgttcag tattaatccg
    12301 aaaaaaacat gaaaagaaaa atactgttga tttgtcttat tgtcacaagc gtattgcctt
    12361 ccatggcaca aacgactact gtaacacaat acgcaaattt ctataccaac gatcagagct
    12421 tatggatgca gggaaactcc ggtctgctgg atctcgacat tccctttttc gatgtttcgt
    12481 ggaatgaaaa cggatccatt ggcggaatca caaccgttgc aggataccca tttggagcgg
    12541 tagtcaatgc tggcacatgg ggccagattg gttccggcct cagaatagaa tttggcaccg
    12601 aaaaagtcga tgtggtgtat aataccgacg taaatctgac catgcccgcc gacggcacct
    12661 ttgaaaaagg cgacgaaata accatttcaa cagcacttga cccttatgaa aacaccagct
    12721 ccataacacg cgatgaatac aacgtgattt ttcagatttg gatggccatc ggatttggat
    12781 ttgacatagg aggtcaattc tgtttttttg attgcacaaa cgggaccatt gttgacatcg
    12841 acttacctgt cgatactttt aatttagtat atctcgactc aaccggggtt tctttacttg
    12901 aaggcctctg ggaatggcct ttttcaaacc ttcccatttc ttttagcgac aacaaggaca
    12961 ttgttacctt aacccttgac ctgccaagca atgccaatga ttcaagctgg ttttcgggga
    13021 acaacatgat ttcggccagc agaccttatg aatatgccag tcttgaattc aatgtttgta
    13081 aattcatcgg agcattaaac attccatacg tcagtgctgt ttttgccaat cttgaaaacg
    13141 aatgggaact cggacccgtt accatcggat atactttaat ggatatggtt ttcaaactag
    13201 ggctctatca caatcaaaga ttaacattca gcccgaccgt aaaaacaaca cttacctttc
    13261 ccggaaacat tgactacaaa gtactgaagc cggacaataa tgtgttatcc tcaggaactg
    13321 cttcatctat tacactggat gtcggaaaca aattacgatt ccgatatccc tgtaattatg
    13381 attttatgga tattgatccc tattatgtca tgcgtaataa tttcaataac cacacttatg
    13441 attccattgc tttcgatttc attttcgata tgcttgaatt ccatgtttca atggacgata
    13501 tagaagtgat tcctgaaata tgcatcccta tttattatcc ctgtggtcca tggtattgtg
    13561 tggtttgtga ctggtgttat gatggcgaca tctgtacacc ggctgttgtt tttcatggtt
    13621 tcaactgggg attcggacct ttggttcatt tgcagccaaa cattttcaat gtcaaataca
    13681 attgggtaaa cagcaactgg gaaatgcaag atttcaacga tgttgacgga gatattttcc
    13741 gcatcacacc ggccagattc gaagtcagtg ttgccccatc cgatgcgccc tgctttggca
    13801 taaacggagg ttctgccatt gccaccgtta cacatggaaa accgccttac cgctacgaat
    13861 ggtcgaacgg taccgttcat accaatgtgg tctcactcac agatgcagta tccaatctac
    13921 cggccggcac acattatgtt attgtaaccg atattaatgg atgtgtaacg ttctgctcat
    13981 ttgttatcgg acaacccgaa gctgctttaa ccatcgatgc cataagcacc aatatttcat
    14041 gcaacggcac tccaagcgga tcaatcaaca tttcgacttc tggtggaaca acgccctaca
    14101 gtttcaactg gagcaatgga gccactaccg aagacataac aaatgttacc gccggaacat
    14161 acattctcac tgtcactgac agtcttggat gcaccaatgt ttcatcctac actcttacag
    14221 aaccataccc actcatttca acagcttcat ccaccaaccc cagatgttac ggagctgcat
    14281 caggttttgc tgcagtaagc tcaacaggtg gaacacctcc ttacaattat ttatggaaca
    14341 ctggtgacac aacacaaagc atcagtaata tcactgccgg tgcttacagc gtaacggtta
    14401 cagattttaa cggctgcgaa aatattctaa cctatgctat cacccaaccc gctgatcctg
    14461 tttcaatcat catcagcact caggatgttt tctgcaacgg agaatcatcg ggatctgtta
    14521 ctgtttcagc gtcaggcggc acagcaccct atcagtacga ttggttccgt aacggtcaat
    14581 cactcaatca tacagcagcc aatcttggca atattgatgt cggcgactat cgcgtaatcg
    14641 ttaccgatgc caatggttgc acaaaagata caaccgtaca tatttccgaa ccccctgcca
    14701 ttgaattcaa tgtggctgtt gtcaacaatc tctgttttgg tgaaaccaac ggaagcatct
    14761 cgctcagtgt aaacggaggt accggcacat atacctacaa ttggtcgaac ggagcttcaa
    14821 cacaaaacat cggaacactt ccggctggca cctatcaggt aacaatcacc gacaacaatg
    14881 gctgccatca atatgctgaa gcaatcatta cacaacccgg agcgccactt acagcaacag
    14941 cagaacccac tcatgtactc tgcaaaggca acaacactgg tgttgcaaca ctgaaccctc
    15001 aaggcggaac agctccttat ttcttccttt ggtcaaatgg ctctaccaat caaaatccaa
    15061 caggacttac tgcaggaaca tacactgtta ccattaccga caataatggc tgtattgcat
    15121 atagcggaac agtcattgaa gaaccaggcg actcgctggc tgcaacattc aatatttctg
    15181 atgcattatg tttcggttca tctgacggaa acataacagc gatggcttca ggtggtacta
    15241 caccgtatta tatacgatgg gatgataccg attatctgat gcaaaataac gggcacaaaa
    15301 ttgaaaacct ctacagtggc tattatcaga ttatcgttac cgatgccaat ggttgtaaat
    15361 cggctcagac actgtttgtg aatcaacccg acagcataca ctcctcagcc attgaaggca
    15421 ttacaagttg ctggggcggt aacgacggaa tcatcgatct ctcggcatca ggaggcacgg
    15481 ccccatacgg ctttatctgg tcaaacggac aaaccagcga ggatgtcagc tcggccgaaa
    15541 caggcacata ctcagtaaca ataaccgatg atcacggatg caccaaaaca gccacctatc
    15601 atattggtac catgcccgaa atcattgtaa atgcatccgt tgttcaggtc agttgctccg
    15661 acaacgctga cggtcagatt acattaattg tttcaggcgg ggccggcagc tacaattatc
    15721 tgtggtcgaa cgaagccacc agttatcagt taagcaatct ggaacccgga agctattctg
    15781 ttaccgtaag cgatctgaac ggttgtacaa aaataattga atacacactt cctgagagtg
    15841 aaactgaatg tatcaacatt ccgacaagtt tcacacccaa cagtgatggc aaaaacgatg
    15901 actgggtcat caataatatt gatttgtatc cgggacatcg ggtgcagatt ttcaaccgtt
    15961 ggggcaatct gctctatgaa caatcgccct atacaacgcc atgggatgga aaattcaatg
    16021 gcaacccgct tccagccgag acgtattact acatcatcga cctcaacaat ggtcaggaag
    16081 catttaccgg aacagtaacc ataatccgct aatcaatgaa gatgaaatta atattgagca
    16141 tttcagtttt tttgtggctt accacagcat ttgccgacaa cggtgaccct gtgaaaaatg
    16201 aagggaattc acccgccccc tgcttttcag tttccttaac cggagatcca aatccctgct
    16261 acggggacag cttaggcacc gttcatttaa ccattaacgg aggtaccggt ccattcacca
    16321 tttcgtggtc atcaggtgaa acaggagtct atgatatctc cggaaaacca gccggatact
    16381 acgacgtaca ggtcattgac cagggtactg gttgtgttgc atttgatatt tttaatgtaa
    16441 tagaaccgac gcaacttact tcttcactga ctcagaccga tgtaaaatgc ttcggacaaa
    16501 gtacgggtag tatcaacctc actgttgccg gagggaaacc cggatacaca tttctgtgga
    16561 atgatggcgc aagcacacaa aacagaatca atctcgacag cggattatat attgtttata
    16621 tcactgatgc aaatttatgc cgtgacacga ttgaaacaat catacagcaa ccggcacaag
    16681 cactcagccg atcgcttact tatgaaaacc cgctttgctt tggcgatttt aacggatctg
    16741 ttgatcttac tgtctgggga ggaacaactc cttatattta cgactggaac acaggaactt
    16801 ttgcttcaca ggatttatcc ggaatcaatg ccggtactta caatgttgta attaccgatg
    16861 ccaacaactg tatttcaaat gcatcagtaa ccctcacaaa tccacctcaa cttacactgg
    16921 ctgttacttc aaccgacaac aactgttttg gagaacttga cggaaccgtt gatgtaacgg
    16981 ttggaggcgg aacattgcca tatcattaca cctgggcaaa tgaaaccttt gttctttcct
    17041 ggaccgacga agacctggac tctttaccca atgacaccta ttatgttact gttaccgatg
    17101 ccaatggctg ccagctaaat aattccgcaa caataagctc tccggatcaa ctgacaatat
    17161 ctctcagttc gagcaatgtt actgcatttg gtggctcaaa cggatctgtc agcagcactg
    17221 ttggaggagg cattgttcct tacatttatt cctggtcaaa cggagccacc actccggatc
    17281 tgaacaacat tccggcaggg tggtatattc taaatatcac tgacagccat ggttgttcta
    17341 ttaccgacag tgtatatatc agcgagccat ccgatcccct cactgtatcg ctcagtggaa
    17401 cacttataac ttgttttggc ggaagcgatg gaatctccga agccatagga tcgggaggaa
    17461 cagctcccta tactttttta tggtcaaccg gaagcaccga aacacgggta accgggcttc
    17521 aggcaggatt ttattccatc acaatcactg attattatgg aaacacagca tcagacacca
    17581 ttgaaattct ccaacccgat aattttgttt tcacacaaac aattacagat gtcacctgtt
    17641 tcggacttgg caatggaagc atcgatgtaa gtataagtgg aggaacagcg ccctacacct
    17701 atgaatggct gaattcgcaa tacgttcttg ctgcgattac cgaagatata tcaggaatgc
    17761 ctgctgacca atacatgttg cagctcactg atacttttgg ttgtacagga agtcatacgg
    17821 taatgatttc tcaacccgac ctgcttgtag cagatatttc atacaccgat gcctattgcg
    17881 ccttcagtag tactggaact gcatcggcag acgtttccgg aggaacagcc ccctacatct
    17941 atctttggtc gaacagtgaa actatggcag atatttcagc gctgacagcc ggaacctata
    18001 ttctgacagt aactgacaat aacaattgtt ttttcacaga cagtgtcatt atatcagaac
    18061 ctgacagcat agccatcaca tatgcaacaa caccggtttc atgccgcgat cagcacgacg
    18121 gaaccatttc gacctatgcc agcggtggca acgggaatta tgaatattta tggaacacag
    18181 gagatatgac tgcttcactg agcgatcttc ttgcaggtat ctattccctc acagttactg
    18241 atatgatggg ttgcacaggt catgccgata tagaagttac ccgcatagaa attgactgca
    18301 tcaatattcc gacatcattt acgcccaatg ctgatggaat caatgatagc tgggtgataa
    18361 acaacagcga gctatatgcc gacgcaacat atcagatttt taaccgatgg ggacaggaag
    18421 tttactccgt aaaaggaatc tgggctccat gggacggaac ctggaacgga aatccaatac
    18481 ccgcagaaac ctattattat ttcatccggc tgacccctga cagccagacg ttacagggaa
    18541 caatcaccat cgtacgctaa gaatataatt gttatgaaaa aaatatttct tcctttgatt
    18601 ttaatgctgt ttgctcaagc cactcttgca cagcaactgt cactcaacag tcagtacatg
    18661 atgaatcgtt ttgtgctgaa cccggccata gccggcaatt acaaccacat tcccattaac
    18721 ttcaacatcc gcaaacaatg ggatggaatc aacggagcac cggttacaca gtcgctttcg
    18781 gctcactcct atcttggcag caacgttgga ttaggtggtt atttcttcaa tgatgtgacc
    18841 ggcccgacaa ggcgtacagg gatgaatgtg tcggtttcct atcagcttaa actgaacagc
    18901 gacttttcgc gcaaactcag cttcggtctg tctggtgtat tctttcagca tttcgttgac
    18961 aaggataaac tcacgaccaa cgagccggat gatattgcca tcaccaatgg cttcaacaac
    19021 cagttctgcc ccgatgccaa ttttggcgtt tatctctcac aaaccaacaa gtactatctt
    19081 ggattctcca ttcagaatct gctcgaagta aagaaagatc tgtttgacat gaaccgcctg
    19141 gtcgacaatc ccgtcgaaag gacctattac gccaatggcg gatacacatt caagcttaat
    19201 gatgattttg ttttcgaacc ttcgttcctg ctgcagtttc aggaaaccac tcccattcag
    19261 atggacttca acggaagatt cggatacaaa aataaattct ggctgggtgg ctcctatcgc
    19321 atgaatgacg cagctgttgt aatggtcggc cttgatttcg acgatttctg catcggctat
    19381 tcctacgata tcaccatgag cgatatcaaa aactattcga gtggcagcca cgaaatcaac
    19441 cttacatacc gcatttacaa aaaaggtgca ttaaggcaga attgcaagag caccccacta
    19501 ttcaactaag atttttctga tcatatgacg cgtttatttt tttcttcagt actgatttta
    19561 ttgatgtcag cacgcctttc agctcaatcg gccaatgcgt ccgaactcat gaacaaaggc
    19621 tgctatgcag aagcttctgt tttgtttgaa aaaacagtga cgggaaataa tcctgaatat
    19681 tctgacctga ccaatcttgc atattgttac atcatgatgc atgattttgt caaggccgaa
    19741 gctgtctatg aaagaattct gcaacatccg aaaaaagaaa atatccagca tttttattac
    19801 ggcgaagtac tgcggataaa cggaaaatat gacaaggcaa aagaacagta cgacatctac
    19861 aaaaccgcat tcccggatga tttcaaggcc gatcagcgga ttcgctcatg cgacagcatt
    19921 gcattatgga acaaaaacaa aacgaaatac tcaactggaa aaatttttga tttgaatacc
    19981 gacaatgacg aaatgtttcc atggatggca gatggcgtgc tgcatttcag cagcaacagc
    20041 cgtttcctgc ttgaaaaaaa cggagcaacg agcaattata aagacccgca gattttcaat
    20101 attttcgttt ataaaaacaa ttccgttgct ctgaaaaaaa ttgcagcaaa ggattcaagc
    20161 agcttcacgg cctatgttcc ggcctatggc aaagaattgt tgctgacaaa aaaaataaaa
    20221 gtcacaccaa ccggtgaaga gttgagtcct tccgaaattt ccgtggataa aaaaacattc
    20281 attccctccg gcgctccgca gggatacacc ttttcgcatc catgcctggc cggcaatggc
    20341 aaacgaatgt atttcgtttc agatatgccc ggtggctttg gcggtaccga tatttattac
    20401 tccgattttg atggcacatc atggagtacc gcagtaaatg cgggaaacaa catcaacaca
    20461 cccggcgatg aaatgtttcc ttacatcact gcccacgatt cattattgta tttttcttcc
    20521 gatggtcatc cgggatacgg aaacatggac atctttgtat cgcgcatcag cagcggtaaa
    20581 tggagctcac ctgtgaatat gaaagccccc gtcaacagca tcggaaacga cttttcaatg
    20641 gtatataaaa actatccgtg ggatggttac cttgtatcaa accgttatcc tgactcgaaa
    20701 ggcggcaatg atattttcag tttcagtttt gagaaaccaa ccattatcaa accggtcgat
    20761 accacaaaac catttgtgta caatgccgat ccgagtcttc tgtacgcctt ctttgagacc
    20821 ggctcaagcg aaatcgcacc ggaatacaaa agcatcatgg acagcctgat cggactgatg
    20881 aaaaagtatg attacctgaa tctttctgtc acttcgtttg ccgatgtccg cggaaccgaa
    20941 aaactcaacg ctcagcttgt tcaggagcgc atgaatgctg tttcaggtta tttttcttct
    21001 gccggaatca gctcttcgcg aatcattggc actcccggca aaacagcatc ggaccgcgaa
    21061 ataccaaatc tcacatttca cgtgcagatt ggttttgtcg ccaaagaaaa tcaggaagcc
    21121 tattttgaat ggagaaccta caataagtac aaagtttcat cttttaaaaa gaaaaacggt
    21181 tacacctatt tcaccgggaa cggcagcctg aatgacatgc aggcgcttgc aagagacatc
    21241 aacaccctat tcaatctggg tgcatatgta attgtttcct atcgcggaat cgttttcgac
    21301 gacacctttt atgcgcccaa ccgacgcgta gaaatgaaat tattcaacaa ataaactcta
    21361 caaaagagcc cgcagtttct tgtatccttc gcggctggca cgaagcttct cgcctgtttt
    21421 cagcgtgaca agatgtgtgt ctttttcata caattcaacc ttgtcaatgc attgaatatg
    21481 gacaatgtaa ctgcggtgaa tgcgcagaaa ttcttccggc agattcttct cgaaatagtt
    21541 catggttttt tctttcagaa aatcaccttt gctgcaatga atcagcacat aatcatcctg
    21601 cgattcaatg aagcggatat cgctgacctg aatgacatgc agattgctgc caaggcgtgc
    21661 caccactctg gaaacctttt cacccgcttc ttcgcgcaat gattctgttt cggaaatcac
    21721 tttatcttgt gccgatccgc tttgtttctt ctgaagaatc agtttatcca ccgcttcgtt
    21781 gaaacgcgct tctgaaaacg gtttcagaag ataatcgacg gcgctttttt caaatgcctt
    21841 tacggcgaaa tgatcatagg ctgtgcagaa aataacagcc ggcctgtact ccagcacttc
    21901 gagcaattcg aagccatcga ctttcggcat ttgtatgtca agaaatatca gatcgggtct
    21961 tagctcatta atcattttga tggcttcgaa accattctga cattcgcctg ccatttcaat
    22021 atcagcccgc tgagcggcgt atgaagcgat gatatcgcgc gccggttttt catcatcaat
    22081 tataataaca ctgagtttca tcgtttcgga atttttaatg tcactttata aacatttcca
    22141 gaatcactcg ttctgaacaa tgaatcatcg ttatacatca gccgtatgcg accggcagta
    22201 ttgctcagac ccgtaccggt gcctgttctg gtgcgacctt caggatcata gctgttttca
    22261 attgtaatca ccagaaaagg atcgctgcat tcacagatca cctgaatggt gctttgctgc
    22321 tcggtactgt acacaccatg cttcacagca ttttcaatca aaggctgcaa aatcagaaaa
    22381 ggcattttca tgctgaggca tttgtcaggc gcatgaatgg ccacattcag ccgttcgcca
    22441 aaacgcactt tttcaatatc ggtgtaaatg cggatatgat taatctcctg ttcaagcgtt
    22501 gaaaactgtt cgtcgttttt gcgcagcgaa tatctgagga gctcactgag tttaatgatc
    22561 atttcgcgcg cctgttgcgg gtttgcaatg gtaagtgaac tgacggaatt aagactgttg
    22621 aacaaaaaat gcggattgat ctgccatcgc aaagcgttga gttcgctttc ctgcagcagc
    22681 gattgcaggc gcattccctg ctctgttttt tcgcgcagca tctgcaggtt tttgtacagt
    22741 ctgaatgcaa gcagcatgag cagcaacagc atggccgcca caatcatgcg cggaatgcgc
    22801 gacagctcat agagcgattt gtacgcttcg ttcgattcag aaatgtattg cagcaacgga
    22861 tcactaacta tataaaaaag agagacagcc accaggtatc caaataccat ctgaaaaatg
    22921 gaacgtgccc gtgtaaaccg cttgtggtct tcgtattgaa gcataaaata caaagcaagc
    22981 ccgttgataa aaagcaaggt tgtgctgatt acactatcag ctgcagccca acgaaaatcg
    23041 atattgtaag ccatccacaa caaaacagca tagaatactg taatcagtat ccatgctgaa
    23101 aaatatgtga aaaaaatatg gctccgactt ttcatttagt caagaatact ggtgatttca
    23161 cctccgccaa acaccgcact gcccctgaca acaagcgttc ttgtcatatc aactgtacca
    23221 gtcacaatcc gcttgtcaac aacaccccca aagacattcg atgtttcgat tttcacattc
    23281 cagtgagcag gcacaatcag tttaaaacca ccaaacaccg ccgaaatttc aagaatattg
    23341 tctccttcgg ccag
//
LOCUS       DAOPKE010000041        23221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2331_length_23221_cov_11.270224, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000041 DAOPKE010000000
VERSION     DAOPKE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_23221_cov_11.270224"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..320
                     /locus_tag="PKY63_06900"
     CDS             126..320
                     /locus_tag="PKY63_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00377.1"
                     /translation="MQYFTSYTGLQNVLKANAVDLNDQAMIYQVLSKHILNQLRKPFH
                     RLRYPEIKNAGDDKLQVVNR"
     gene            532..981
                     /locus_tag="PKY63_06905"
     CDS             532..981
                     /locus_tag="PKY63_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF00378.1"
                     /translation="MKIEKISENKKQFLDLLLLADEQENMIDKYLYDGDLFALYDGDL
                     KSICVVLPIDSATCELKNIATCEKYQGHGYGSALIQFISDFYKKTFSFMLVGTGETPQ
                     ILSFYERCGFEMSHRIPNFFTDNYNHPMFDGDIQLVDMIYLKKNLRK"
     gene            1096..1593
                     /locus_tag="PKY63_06910"
     CDS             1096..1593
                     /locus_tag="PKY63_06910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPF00379.1"
                     /translation="MQKKSLVVIDIQNDITKNYKEIIDNVNRSIDWAVENEIHVVYIK
                     HYNLSAGTRTFKPDTRGSELVSDMKLVSENIFEKSKGNALTSEAFTDFIRKNEISEFY
                     IAGADAIACVKSTVFNMRKENYKVTVISDCITSYDKRKIDEMLKYYEKNGSRIIKLKD
                     LLDSK"
     gene            complement(1896..2273)
                     /gene="ssrA"
                     /locus_tag="PKY63_06915"
     tmRNA           complement(1896..2273)
                     /gene="ssrA"
                     /locus_tag="PKY63_06915"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2487..3647)
                     /locus_tag="PKY63_06920"
     CDS             complement(2487..3647)
                     /locus_tag="PKY63_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPF00380.1"
                     /translation="MSSILIIDDEQPIRKTLREILEYEKFKVTEAENGIKALELIRAN
                     KFDVVLCDIKMPQMDGLEVLEKIQELSDVPVVMISGHGNIDTAVEAIKRGAFDYISKP
                     PDLNRLLVTVRNALDRHSLVTETRKLKQKISKAYEIIGESPAIMQIKEMIERVAPTDA
                     RVLITGENGTGKELVARWLHELSARAGNSFIEVNCAAIPSELIESQLFGHEKGSFTSA
                     IKQRKGDFELASGGTLFLDEIGDMSLSAQAKVLRALQENKITRVGGEKEISVDVRVVA
                     ATNKNVKDEISKSNFREDLYHRLSVIIIHVPPLRERLEDIPLLVEHFANDIAITQGKP
                     AKEFTNKAIDELKKLQWTGNIRELRNVVERLIILSGKEITDKDVKLFARPLA"
     gene            complement(3977..4681)
                     /locus_tag="PKY63_06925"
     CDS             complement(3977..4681)
                     /locus_tag="PKY63_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF00381.1"
                     /translation="MEKKKGRILLVEDDRTLSTLVRDILEINKYEVVLCSNGEEGLAT
                     FKSQKFDLMLVDIMMPKMDGITMIKEIRRYDQRVPIIVISAKDMKDDKVEGFRAGADD
                     YVVKPFSTDELLLRIDAILKRVQRAQEMLLTTTTEIIEFGNCKLDVGEQNLTIKNKTM
                     HLTRKECDLIRLLAMKRNELLLREVALKAIWGDDDYFIGRSMDVFISRIRKYLKDDNK
                     VEIVNVHGLGFKMMVK"
     gene            complement(4687..5949)
                     /locus_tag="PKY63_06930"
     CDS             complement(4687..5949)
                     /locus_tag="PKY63_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF00382.1"
                     /translation="MSAKVYKFLIYISISAMIAVIVGQLLWTHYSRTVIRQSGYTLIQ
                     LSLAKSSHDFGNRMAFIELKTGEKPAFEQMRFVIDSILRSEFESIIILENLEYSIYRG
                     NQILFKSNDGISDEDVFNSELSRGFWYEKNKPAIMVSVIAPDRIGYYLNLSNMNIWWL
                     ISMLGFATIAFISLILLRGMQKAKRDANTRVKTINNIAHEFKTPIASIKLASEMLIHE
                     DVARQPERVNRYAELIQYEVKRLQSQTEQFQNVVLLEEGQIMLRFNYLNANEVLQKLV
                     NHYVLVRGDYQDRLFFDLKAENDFVYADLAHFENVITNLIDNAFKYGGEKVKVTVSTR
                     SNEDAVFILISDDGYGIAKEHQKLIFERFYRVAPINKHDVKGHGIGLYYVKTILKQMG
                     ADISVYSSPGKGATFEIVFPFELNNQEK"
     gene            6033..6443
                     /locus_tag="PKY63_06935"
     CDS             6033..6443
                     /locus_tag="PKY63_06935"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPF00383.1"
                     /translation="MTSKVRYIGELRTVCEHFASSSSIETDAPIDNHGKGEKFSPTDL
                     VATSLASCMLTVIGLSGQTLGFSIDGATASVEKFMGTDPRRIVKIRIIIDFPGMSFSE
                     REQQIIRHAARLCPVGQSLHPDLEQEIIFNFEKK"
     gene            6447..6821
                     /locus_tag="PKY63_06940"
     CDS             6447..6821
                     /locus_tag="PKY63_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPF00384.1"
                     /translation="MLQSGLTYSLEKKVTAEDSANTYGSGMVDVLATPAMIAFMEKTC
                     LLLALEHLEPGFNTVGIAVDITHNKATPIGMNMRCIASLLEIEGKILKFHVEVFDDQG
                     KAGAGFHKRAIINVEKFMAKLK"
     gene            6818..7438
                     /locus_tag="PKY63_06945"
     CDS             6818..7438
                     /locus_tag="PKY63_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00385.1"
                     /translation="MKKIEFIILFVLASCPAAMAQYDSSAVYPDFRFNDGIYLTFDDF
                     KNNRPVYTDFEIKYRGGATYLYIMCADSSRSYDCEVKQPWGYCLNKSVFIHQGYGDNY
                     FRLQVIGALIHYFVIEMQYYDPAFNDPFNPYSTVPNRRITNRELVMEWTTGRRFEFCY
                     KNFKIFLQEKDPQLLQQLESTKKKRKMIYFFMLKYNEKHPVFIKQQ"
     gene            7440..8903
                     /locus_tag="PKY63_06950"
     CDS             7440..8903
                     /locus_tag="PKY63_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HPF00386.1"
                     /translation="MLAVFIIYIAIVIALAIYFSLKAKSSTDFVMGGRKIPGFALALS
                     ERAAGESSWLLLGLTGLALAEGFSAVWVALGCVIGILFIWWIMADRLRKSSIETGANT
                     IPSLFATRFPNHAFSIQMLSSLIIIFFFLFYIAAQFSGSGKVLFMTFGLDETSGGLVS
                     AAVIILYTMLGGFVTVVAIDVFQAILMFFSIVVIPIIAIFIIASLDISFAQTLSQAAP
                     AISSLNAGKEGTSAWLFILSGLSWALGYTGQPHLLARMMAISNKKHVNIARKVASLWT
                     VIAYIGAILLGLAGYVLLNNSYFTPAETLAISNDAEKILPTMVSCLVTPLMAGILLSG
                     TVSAMMSTAASQLMVCTFCLTEDVGPHVKRKNKLLLPLSNVWFHRLIIIIVGVVALVT
                     GMLMSDTVYGLVSYAWAGIGSSFGPALLLFLFWKKFSAAGVFASLIGGTIGAVLWKMF
                     FSEPTGISERLVSFVFAFVMAIVFSLLFPTETNKTKA"
     gene            complement(9233..9952)
                     /locus_tag="PKY63_06955"
     CDS             complement(9233..9952)
                     /locus_tag="PKY63_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF00387.1"
                     /translation="MLKVIIVDDEAKARKSLALLLEKSGSAEIVASVENAQEAIRAIA
                     TYQPDIVFLDVKMPGMNGFEMLDAIDRIVLREFDVVFLTAYDEFAIRAIKYSAFDYLL
                     KPVDETELRETLDRYISNVHPLQDYDALKATLNQNSKIKIRTNQGFEYINAIDVVFIE
                     GDGNYSKFVLDQNDERIVSKTLKDISVDLTDDFLRVHKKYFINKNYLVSINRHEHICL
                     LQKGDIQFRIPVSVRMMKHMP"
     gene            11015..12733
                     /locus_tag="PKY63_06960"
     CDS             11015..12733
                     /locus_tag="PKY63_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00388.1"
                     /translation="MNYYKYNQENPIFVSKNKAMILKKIILSILVLLITTQLFAGTGK
                     VDFIECTVDLQPSGKAVIGYCVRYTVVEGELHGFYFSGLDRLEIESFSDKNYAIDKNG
                     NRFDLSISNVGGKKYDIVLANGKGIGSGYVTYFFWFETNFSSAGYLGTTTSENGKNLV
                     YFDWAPTEWDSDATGMHHYTLKILTPFKPSFSFNVRERVYADSLIMTEKWMNEKYEID
                     FQIKEAGRLMMLVNKNTPEYHFSPRVQIYMPAAWFDTSMVREYQIRTDETSNGTQKQS
                     NDSYNYQQPQQTLNPTTLGILAAVLLLIFVLYFVLKNKGLANARENADNIGWDKLNWI
                     PPKLEMATYSIPGKICDNLNYVEIAFYLEFPIKKIFSSMLAAFEAQQYITVLKNDPLT
                     IKIIEMPTSFDNDYEELFFNALTDDGVFSQEELNAIMNKAIANIREKAWDADVDATKK
                     YYSELIQSKTDDYEKANPSQKMHVQDNTDLMYNYWTRYYHYYHPYFTYQSIMNPSRYE
                     QYFPVNTDHVVYNSIHSDIIPDINACHAACHDACHSACHSACHSACHSACHSACHSAC
                     VSGGSH"
     gene            12745..14079
                     /locus_tag="PKY63_06965"
     CDS             12745..14079
                     /locus_tag="PKY63_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF00389.1"
                     /translation="MDQRFNWVKEFIASVKPYIEVRLEDNMLIKKPNNVQKLNPSGAL
                     ILYQLLNGSSIDGLIRKIGNDEKRLNELLSFLLAVKSSLEGNLDVWTKNTAVVKAAFE
                     APFTKLPVLSELAVTGKCNLRCSFCYAGINYGCTTETTDLRVDQLKQLIYKIRHQGKV
                     PSISFTGGEPTLRAELPELIAYAKSLDMRVNLISNGFLIDEAMALGFKNAGLDSAQIS
                     LEGITQETHEKITGVGGSFENALNAVSALNAQKIRTHTNTTLSKQNMHEVDGLPRFLK
                     HHFNAERFSMNLIIPTGSALANEMHLLQYSEAGHVIRRVYNASLLENVEFMWYSPTPL
                     CIFNTITEGLGNKGCAACDGLLSVDPEGNILPCSSWNEPLGNLITQDFESIWFSKRAT
                     EIRNKKSAPEICNGCDSLAICQGACPLFYRAESNHDRELMKARISNITHTVL"
     gene            14076..15008
                     /locus_tag="PKY63_06970"
     CDS             14076..15008
                     /locus_tag="PKY63_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00390.1"
                     /translation="MKKLFFHFFPRMSNSLRVIVFISLFAAGIAIQLTIANFWIGAIL
                     IACASLILLNKGIDSRLHIERYHHNTEWKTSTRQQVEDIIKMDKKLRRWDRTGFEVTS
                     CLGSFLFLVVLVAGFFIVILGLEENDLNVIFFGGDFLILFIPHFLSGFRRIDMASRVL
                     LYADNCLKAADIAIELNKNVKTDFMTLLAENAKTKALFPKEVKLKMSLENAPDTFLGC
                     YGQLSVNKVGSKDYPYFYTVLIFKPEFDLKKKFNKLKLLNKGMLIEFTSEQGVEVLVL
                     RQVTTRTSGYSTNNKAVRGILVQTFDAYQQLMSM"
     gene            complement(15181..18102)
                     /locus_tag="PKY63_06975"
     CDS             complement(15181..18102)
                     /locus_tag="PKY63_06975"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF00391.1"
                     /translation="MKARLFIILQVIFFSGFAQQYSYRHYTIFDGLPQNQVVNVFQRS
                     DGIFLYGTKNEIGGFDGSRFRTYNDRYITKSFVEGFFEIGDQFFYYTREALVRFENKQ
                     SSLIKDFNGFGVHTVIVNAGLREIYFLSDTALCVIHPGRENFYYKNHDMGFIADMERI
                     PGSCDFLLSTGKGVYRLDCNKQLTPVFTGQGNQIVVVDNKLFVGVVRNNKLPSEFDGI
                     YCFDENQPRKIYAYGNNIPGAVMLKTKNNQLLINQNSSVWIRLDTSGHILDRDSIPDM
                     IINDIFEDCNGNLFIGTENGLLHQQSYAFRNYDWKSAMPRYVWSIFEDKDSTLVFATF
                     HGRLFQLRNNIMSEVQGYRKNMDINEVFYMGGFCNSLGQWMIPTNYRIFINDHGQYRF
                     LPLLHEGNFSTCLATFEDTIEKNVYFGTTNGLFIYNLLSGKLKHVDTKGYNILAIEKD
                     RNDDYWICTNKLVYLLKNDSILQTADHSGLPNVGVISCKKDTLGNMWYARKDGLYLYN
                     YAGHIKISEGHYFFVSLWKNKKIIAGGVEGISVIDLNMFYAKNPSALQHFDRFNGFLG
                     IECGQNGTCIDSKNHVWIPTSESVVRFDPEKTMYDSVPPTPFIVSFERSSKSLLWKDI
                     SIAWKPTNAVYHLDPGESNIRILYDGLDYVSLEKMKFRYRLIGYSEYWTETTDKEAIF
                     TNLSPGFYQFELTVANGNGIWSKDPHILKFNIKPAFYQTWGFRIGVAIFDLLAVAGIV
                     YLIMRRKQKRKNQERQVQKELVAMQVKTINAQLDPHFIFNTITAIGSEVQEKNNDKAY
                     SYFVKISQLLRNSIKDTDKIIRTIEEELKFVENYLSLQKFRFEERFNYSIQVDPLVSF
                     KQKVPKMCVQIFVENAMKHGIEQLMHGGLLRISISKKQDKTVFIVEDNGVGRAASAKN
                     GTQSTGVGLLVFSEFFEIMNRYNDNPAGFVIHDLKNENGEALGTRVELTIPDSYQYEI
                     Q"
     gene            complement(18726..20339)
                     /locus_tag="PKY63_06980"
     CDS             complement(18726..20339)
                     /locus_tag="PKY63_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00392.1"
                     /translation="MSLFKAIILFAFLPVCFSAQAQQLMLPMNTAFSADIEEAIYSPD
                     TSFHTSIKPFVEADVRKVFDLDSSRSAQLFSNPAQRKFGSWLFRKAFNEHFFQVRTDD
                     FYLDIDPVFAFEPGKDFSAIEDLRFVNTRGVQAQGSIGKKFSFFTSFYENQATFVDYI
                     DERIRNTRVVPGQGMARSFYSSGFDYGYSSGHISWAPSKFLNIQAGHGKNFYGDGYRS
                     LLLSDYAFNYPYLKLNVEAWKLKYSIMYAQFQDLKVPHGGNIGFQKKYGTFHFLNYAV
                     NRRLQLGLYEAVIWINEDSTGHRGFDVNYLNPVIFFRPVEFSLGSPDNVLIGLNASYK
                     LRKKNVLYAQFLLDEIKVHEILSGDGWWGNKSALQAGFRTYDIAGLDGLSLQSEVNFV
                     RPYTYSSRTSTLCYGHYNEALAHPLGANFIESVSILKYHYKRFYAQLKYNFIRFGADD
                     DGINYGNDIWQSYTTRPFEYGNYVGQGDKTTVNIVDASVSYLVNPATNMNVTLGLMRR
                     TFDPEIGWRESTFFYFGFRTSLNNLVYDF"
     gene            complement(20402..21538)
                     /locus_tag="PKY63_06985"
     CDS             complement(20402..21538)
                     /locus_tag="PKY63_06985"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPF00393.1"
                     /translation="MNSVEFDIYSVPLYLMLIISFSIALIATWSTIPAIVSLANAKKL
                     YEEPNERSVHKIATPTLGGLAIFAGFKFSYLVFSGQTALPERPFIAAGCLVIFLMGMK
                     DDIFVLAPIKKFIAQIFATLLVVFLGNIYISDFQGLFGINEIPYWASIVFSFFVVIAI
                     INAFNLCDGIDGLAGSLGNIAAVTFGTWFALAGDYVYAIMCFSLAGSLIGFLRYNLSN
                     GKNKIFMGDTGSLLIGFVIAIATIRFIELNRSPLIEHHVSSAPAVAIGILFIPIYDTI
                     RVMSARIMRRKSPFKADRGHLHHKLLDLGYTHPQATLMISFTSVVFIVLSFLLQHIGI
                     LQLLFVLTIIATAWYLIPVTKWARRLSAKVTGKPRSVETGKPCC"
     gene            22458..>23221
                     /locus_tag="PKY63_06990"
     CDS             22458..>23221
                     /locus_tag="PKY63_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00394.1"
                     /translation="MNNYAKIKSLVAAIRKIARNAAYSTSTILLIVISSALHAADINT
                     AGTGNWSSRSSWSQVAGTGTISYTAGSTDVTGTGTAFTTELTVGDIIYTTQPNGLSIA
                     IASITDNNHLTLVSAPYTSSGQRSYTVSRTPVASDDVHIIGGYTITVDISNAYCSSIS
                     IGNGGNKECTLAFNANSQLSVSSSVTLNTTKNNKTILLDMTNGGTLICASLAVPSTGG
                     IQKFTAGSGTIILTAGNTIPAAVFTTFNNLTLQAGTT"
BASE COUNT         6970 a         5084 c         4513 g         6654 t
ORIGIN      
        1 tgaatatatt tctgtttttg tgagtgttac gtgtttttca gcagacccta tttaattcgg
       61 gatcgtttca taaagttgaa ttgcttgctg gcgaaaacgg gctcctatgc ttcaaacacg
      121 atgtaatgca atattttact tcgtacaccg gattgcagaa tgtattgaaa gccaatgcgg
      181 ttgatctgaa tgatcaggca atgatttatc aggttttgtc caaacacatt ctgaatcagc
      241 ttcgcaaacc ttttcataga ttgagatatc ccgaaataaa aaatgccgga gatgataagc
      301 tgcaggtcgt gaaccggtag agcagaacaa aagacattca cttcaacaca tcgccaggct
      361 tatctgagcg atgtgttttt ttgtaccaac aattttgaat gcaattcatt tattcatttt
      421 cactgaattg cttaaccagg caatgattac ttttgtattg caaatgagct atgtgaccta
      481 tacgtacagc aacaacagca aaaccaacac aaaccggaaa acagcgtcat aatgaaaatc
      541 gaaaaaataa gcgaaaacaa gaaacaattt ctcgatttgt tgttgttggc tgacgagcag
      601 gaaaacatga ttgacaagta tttatacgat ggcgacttgt ttgcattgta cgatggcgat
      661 ttgaaaagca tttgcgttgt attgccgatt gacagcgcta cctgcgaatt gaaaaacatt
      721 gcaacctgcg agaaatatca gggccacggt tacggcagcg cattgattca atttatctcg
      781 gatttctata aaaaaacctt cagctttatg ctggtcggaa ccggcgaaac acctcagatc
      841 ctatcgtttt acgagcgatg tggctttgaa atgtcgcatc ggataccgaa tttttttacc
      901 gacaattaca accatccgat gttcgacggc gatattcagc tggtggatat gatttatctg
      961 aaaaagaatt tacggaaata gactcagtat ttgtgctgcg ccggcaaaac gtacatcgca
     1021 tgcaaataag caaccgtgcg atgaacaatg agatagaaaa ttgaacgacg cattaaaaca
     1081 aaacaaggaa atcccatgca gaaaaagtct ttagtggtaa ttgatattca aaatgacatt
     1141 accaaaaact acaaggaaat cattgacaat gtgaatcggt caatagattg ggccgtggaa
     1201 aatgaaattc atgtcgttta cataaagcat tataatttat ctgccggcac caggactttc
     1261 aaacctgata cccgtgggtc tgaattggtt tcggacatga aattggtttc cgaaaacatt
     1321 ttcgagaaat ccaaaggcaa cgcattgacc agcgaagctt ttactgactt tatcagaaaa
     1381 aatgaaatca gcgaatttta cattgcagga gcagacgcca ttgcctgcgt gaaatcaaca
     1441 gtatttaata tgcgcaagga aaactacaaa gtaacagtca tatccgattg tattacgagt
     1501 tatgataaaa ggaaaattga cgaaatgctg aagtattacg aaaaaaatgg cagtagaata
     1561 attaaattga aggacttatt ggattcgaaa tagggggcct gcaacccaag ccacctgctg
     1621 cttcgcatcc agtggcattt ttttgcgctt tcggctgatc gcgagcgctc gcctcaaaat
     1681 taaataatcg aattgtacca taaataaatt ccggtttgta agccgtgctc aaagtcgcaa
     1741 aaggggctgg cttcgccgag cccatttggg gggggccatc aacccaagcc acaagctgct
     1801 ttgcatccgg tggcattttt ttgcactttc ggctgatcgc aagcgctcgc ctcagcgcaa
     1861 aaaaaagcca ctttccgaaa ggaaagtggc ttgggttgtg gagctggagg ggttcgaacc
     1921 ctcgtccaaa cagcggacaa tggagctttc tacatgctta gcttctggtt ggttttcgtg
     1981 tgcaaaccgg ggagaaacgg gccctatctg caccttatcc ttttattttt tagcagacga
     2041 ccaaaggcat atcaatctgc gattccggca tttgcggtgc ctcggtccag acgcggtcgg
     2101 tcagcgcttc cggggagaca aatggggcct aaacattccg cttaggcagc cattgcgaac
     2161 gagttatcgt tgcctgttat tatagtgttg cccgtggttt ttacggagaa tacggacgtc
     2221 ctccggcatg cttacttcac cacctcaact gctgtcaaaa ccaatacagc cccagtagaa
     2281 atgctttgca aagatacaac aaagttgtga gttgtgagtg gccagttgtg agttaaattg
     2341 aggacaagca acattcaatc cgccgtggcg aaacaacggt ctgcacagtg cattgtgctg
     2401 tggaccgcct gcctcatgcg ctaacgtcac ctttaaactt tagaacttta aaaactttca
     2461 actcgtagcg cttgctgcgc ttcgcgctac gccagcggcc gtgcaaacag cttcacatcc
     2521 ttatccgtta tttcttttcc ggaaagaata atcaatcgct caacaacatt gcgcaactcg
     2581 cggatgttgc cggtccactg aagttttttc aattcatcga tggctttatt cgtgaactct
     2641 ttggcaggtt ttccctgcgt gattgcaatg tcattggcaa aatgttcaac cagcagcgga
     2701 atatcttcaa gtcgctcgcg gagcggcgga acatgaatga taattaccga cagacgatgg
     2761 tatagatctt cgcggaaatt acttttcgaa atttcgtctt tcacgttttt attggtggca
     2821 gctactacac gaacatcaac cgaaatttct ttttcgccac ctacgcgcgt gattttgttt
     2881 tcctgcagcg cgcgcaaaac ctttgcctgc gccgaaagac tcatgtcgcc gatttcatcg
     2941 agaaacaaag taccgccgct ggccagttcg aaatcgcctt tgcgttgttt gatggctgaa
     3001 gtaaatgaac ctttttcatg accgaataat tggctctcaa tcagctcgct cggaatggcc
     3061 gcacaattta cttcaataaa cgaattccct gccctggcac taagttcatg cagccagcgt
     3121 gcaaccagtt cttttccggt tccgttttcg ccagtgatta aaacgcgcgc atcggttgga
     3181 gcaacacgtt caatcatttc cttgatctgc atgatggcag gagactcacc aatgatttcg
     3241 tacgccttcg aaattttctg tttcagcttg cgcgtttcag taaccaaaga atgacggtcg
     3301 agtgcattgc gaacggtcac cagcaagcga ttgagatcgg gcggctttga aatataatca
     3361 aacgcaccac gctttatggc ttccacagct gtgtctatat tgccatgacc cgaaatcatg
     3421 accaccggaa catcgctcag ctcctgaatt ttctccagca cttcaagtcc gtccatctgc
     3481 ggcattttta tgtcgcacag caccacatcg aatttattgg ctctaatcaa ttcaagtgct
     3541 ttgatgccat tttcagcttc agtgacttta aatttttcgt attccagaat ttcgcgcagg
     3601 gttttgcgaa tcggttgttc gtcgtcgatg atcagtatag aactcatgct aatggctttg
     3661 tttgcgaaat tacaaaaaaa aattactttc tgctgacatt tcagcgtcag cagaaaaagg
     3721 cagactttaa gtcattgctg acctggaatt ttatctgact ggcaaggcag acgtcgggga
     3781 tataggccat agcacccaac agccagtccg cctgccggcg gaattccggg accgccctta
     3841 tgctcaatta aattgcccgg cacagcttga agcggtataa actcctgtca aaatgttggg
     3901 tgctcaaaaa aaaacctgcc ggctaatgtt tccaatagcc agacaggact ttattattct
     3961 gattttcaga gacgtattac ttcaccatca tcttgaagcc gagcccgtga acattcacga
     4021 tttcgacctt gttgtcatct ttcagatact tacggatacg gctgatgaaa acatccatgc
     4081 tgcggccgat gaaatagtca tcatcgcccc agatggcttt cagagccact tcgcgaagca
     4141 aaagttcgtt gcgtttcatg gccagcaaac gaatcagatc gcattccttg cgggtaagat
     4201 gcatggtttt gtttttaata gtcaggttct gttcaccaac atccagctta caattaccaa
     4261 attcaataat ttcagtggtt gttgtcagca acatttcctg agcacgctga acacgtttca
     4321 gaatagcatc gatgcggagc aacagctcgt cggtgctgaa aggttttaca acataatcgt
     4381 cggcacctgc acggaaacct tcaactttgt catctttcat gtctttggca ctgattacaa
     4441 taataggaac gcgctgatcg tagcggcgta tttccttaat catggtaata ccatccattt
     4501 tgggcatcat gatgtcaacc agcatcaaat cgaatttctg cgatttgaat gttgccagac
     4561 cttcttcgcc attggaacaa agtacaactt catacttgtt aatttcaagg atgtcgcgta
     4621 caagagtact caaagtgcgg tcatcttcga ccaggagaat tctgcctttt ttcttttcca
     4681 taatttttat ttttcttggt tgtttagttc aaacggaaat actatttcga atgtggctcc
     4741 tttgccagga ctgctgtaaa cagaaatatc agcccccatt tgtttgagaa ttgtttttac
     4801 ataataaagt ccgatgccat ggcccttaac atcgtgttta ttgataggtg cgaccctgta
     4861 gaaacgttcg aaaatcaatt tctgatgttc ttttgcaatc ccatatccat catcgctaat
     4921 caatataaaa acagcgtctt cgttggatct tgtggacaca gtaaccttta ctttttcgcc
     4981 accatattta aatgcattat caattaaatt ggtaatgaca ttttcgaaat gagccagatc
     5041 ggcataaaca aagtcgtttt cagctttcag atcaaagaaa agccgatcct gataatcacc
     5101 tctgacgagc acataatgat taaccagttt ctgcaaaacc tcattggcat tgagataatt
     5161 gaaacgaagc ataatctgac cttcttcgag cagcaccacg ttctgaaatt gctcggtttg
     5221 agactgcagg cgcttcacct catattgtat caactcggca taacgattaa cccgctccgg
     5281 ctgacgtgcc acatcctcgt gaatcagcat ttcgctcgcc agtttaatgc tggctatggg
     5341 tgttttgaat tcatgtgcaa tattgttgat ggtctttaca cgcgtgttgg catcgcgttt
     5401 ggctttctgc atgcctcgca aaagaatcaa agatataaat gcaatcgtgg caaatccaag
     5461 catggaaata agccaccata tattcatatt tgacaaattc agataataac cgatacgatc
     5521 cggcgcaatt acacttacca taatggccgg cttgtttttc tcgtaccaga atcccctgct
     5581 caattcactg ttgaagacat cttcatccga tattccgtca ttcgatttaa acaatatctg
     5641 atttccacgg tatatcgaat attcaagatt ttcaaggatt attatactct caaattcaga
     5701 ccttaaaata ctatcgatta caaaacgcat ctgttcaaaa gcaggttttt ctcctgtttt
     5761 aagctcaata aatgccatcc tgtttccaaa atcatggctc gatttggcca gcgaaagctg
     5821 tatcaacgtg taacccgact gacgaataac cgttcgcgaa taatgtgtcc ataacaattg
     5881 ccccacaatt accgctatca ttgcagaaat tgatatatat atcagaaatt tatatacttt
     5941 tgccgacata ctgagggttt caagtgttaa aattaactcg aatataaaca ccttggtgtt
     6001 aaataagtat taaatgttaa aatatttttt ttatgacctc aaaagtgcga tacataggtg
     6061 aattaagaac tgtttgtgaa cattttgcct catcatcatc cattgaaacc gatgccccca
     6121 tcgataacca cggcaaagga gaaaaatttt caccaaccga cctggtggcc acttcgcttg
     6181 catcgtgcat gctcactgtt attggtttga gcggacaaac gctgggcttt tcgatcgacg
     6241 gtgccacggc atccgtagaa aaattcatgg gcaccgatcc ccgcagaatt gtaaaaatac
     6301 gaatcattat cgattttcca ggtatgtctt tttcggaaag agagcagcaa atcattcgac
     6361 atgctgccag actctgtcct gtagggcaaa gtcttcatcc cgatctggag caggaaatta
     6421 tttttaattt tgaaaaaaaa taaacaatgc ttcaaagcgg acttacctac tctctcgaaa
     6481 aaaaagtaac agccgaagat tcggccaata cctatggttc gggcatggtt gatgtgctgg
     6541 ctacgcctgc catgattgct tttatggaaa aaacctgctt gttgctggcg ctcgaacacc
     6601 tcgaaccggg tttcaatacc gtgggtattg ctgtcgatat cacacacaac aaagccactc
     6661 ccattgggat gaatatgcgc tgcatcgcct cactgctcga aattgagggt aaaatcctca
     6721 aatttcacgt tgaagtcttt gacgatcaag gaaaagccgg tgccggattt cacaaacgtg
     6781 ccattatcaa tgtcgaaaaa ttcatggcaa aactgaaatg aaaaaaattg agtttatcat
     6841 attgtttgtc ttggcatctt gtcctgcggc aatggcgcaa tatgattctt ctgcagttta
     6901 tcccgacttt cgtttcaacg atggtattta tctcactttc gacgatttca aaaacaacag
     6961 acccgtttac accgatttcg aaatcaagta ccgggggggc gctacctatc tatatattat
     7021 gtgcgccgat tcttcccgtt cctatgattg tgaagtgaaa caaccctggg gttattgttt
     7081 aaacaaaagc gtttttatcc atcagggtta cggtgataat tatttccggc tacaggtcat
     7141 tggagctctc attcattatt ttgtcattga aatgcaatac tatgaccctg ctttcaacga
     7201 tcccttcaat ccgtattcca ctgtgcccaa cagacgtatt acaaaccgtg aactcgtgat
     7261 ggaatggacg acaggacgtc gttttgaatt ctgctacaaa aatttcaaaa tctttttaca
     7321 ggaaaaggat ccgcaattgt tgcaacaact ggaatcaaca aagaaaaaga ggaaaatgat
     7381 ttactttttc atgctgaaat acaacgaaaa acaccctgtt ttcattaaac aacaatgata
     7441 tgctggccgt tttcatcatc tacattgcga ttgtaattgc actggccatt tatttttcgc
     7501 tgaaagcaaa atcaagtact gactttgtta tgggtggccg caaaatcccc ggatttgctc
     7561 ttgctctctc cgaaagggct gccggcgaat catcgtggct actgcttgga ctcacgggat
     7621 tggctctggc tgaaggattc agcgctgtct gggttgcatt gggctgtgtc atcggcattt
     7681 tatttatctg gtggatcatg gccgaccgcc tgcgcaaatc gagcattgaa acaggcgcca
     7741 ataccatccc ttcacttttt gcaacgcgat ttcccaacca tgctttcagc attcaaatgc
     7801 tttcttcctt aatcatcatt ttcttttttc tattctatat tgcggctcaa ttcagtggtt
     7861 cgggaaaagt tctttttatg acattcggac tcgacgaaac cagtggcggg cttgtttctg
     7921 cagcggtaat cattttatac accatgctcg gcgggtttgt tactgttgtt gctatcgatg
     7981 tttttcaggc cattctgatg tttttttcca ttgttgttat tccgattata gccattttta
     8041 tcattgccag tctcgatatc agttttgcac aaacactgag ccaagctgcg cctgctattt
     8101 cgtcgctcaa tgccggcaaa gaaggaacat cggcctggct ttttatttta agtgggctca
     8161 gctgggctct gggttatacc ggtcaacccc atctgctggc ccgtatgatg gccatcagca
     8221 ataaaaaaca tgtcaacatt gctcgtaaag tggcttcgtt atggacagtt attgcataca
     8281 ttggtgcaat cttgctcggc cttgccggat atgttcttct caataactct tatttcactc
     8341 cggccgagac attggcaatc agcaacgacg ctgagaaaat actgcccacc atggtcagct
     8401 gccttgttac tcccttaatg gcaggaatcc tgctttcagg caccgtttca gctatgatgt
     8461 caactgctgc aagccaactt atggtttgca ctttttgcct tacagaagat gttggcccgc
     8521 atgtgaaaag aaaaaacaag ctattactcc cactcagcaa tgtatggttt cataggctga
     8581 ttattattat tgttggagtt gttgctcttg ttaccggaat gcttatgtca gatacagtat
     8641 atggactggt ttcatatgcc tgggctggaa taggatcaag ctttggtccc gctttgctct
     8701 tattcctttt ctggaaaaaa ttcagtgccg ccggcgtttt tgcttctctt ataggcggca
     8761 ctatcggtgc tgtattgtgg aaaatgtttt tcagcgaacc taccggtatc agcgaaagac
     8821 ttgtgtcttt tgtttttgca tttgtgatgg ccattgtttt ttcacttttg tttcctactg
     8881 aaacaaataa aactaaggcg taatctctgg ttttccaata taataaacga caatttttca
     8941 gacatcagtt cattcatttt gcaacaatca tgaatttgaa ggattattac agcttgccgg
     9001 taagcaatat aggaacaact tcggcaatac tgcgctgatt tttattgtat taaaaaaatg
     9061 atcatcctta ttaattttgg cagagccaac gctttaaaac acacaaaaaa actcctgaat
     9121 cacagtttat accaaaatga aatatataat agtctgattt cattggatga ctccggagga
     9181 gacatatgtc tgtagaaata ataatggttc aaaatgaatg attataaaat ttctatggca
     9241 tgtgtttcat cattctcacc gacacaggta ttctgaattg aatatcacct ttttgaagca
     9301 agcaaatatg ttcatgccgg ttaatggaaa caagataatt cttattgatg aaatattttt
     9361 tatgaacccg taaaaaatca tcggtcagat ccacactgat gtctttcaat gtcttactca
     9421 caatgcgctc atcattctga tcgagcacaa atttcgaata attcccatca ccttcaataa
     9481 aaacgacatc gatggcattg atatattcaa atccctgatt agtacggatt ttaatctttg
     9541 aattctgatt caatgtagct ttcagagcat cgtaatcctg caacggatgc acattggaaa
     9601 tataacggtc caaagtttct ctgagttctg tttcatcaac tggtttaagt aaataatcaa
     9661 acgctgaata ttttatggct cgtatggcaa actcatcata ggccgtgaga aaaacaacat
     9721 caaattcacg caaaacaata cgatctatgg catccagcat ttcaaatccg ttcatgccag
     9781 gcattttcac atcaaggaaa acaatatcgg gctgatatgt tgctattgcc cgtatggctt
     9841 cctgtgcatt ttcgacactg gcaacaattt cggcggatcc gcttttttct agcagcaacg
     9901 ccagactttt tcgggctttc gcttcatcat caactataat tactttcaac atgtgccaat
     9961 tcatttttta ccggaaccaa caaatataag aacaaaattt ctgaaagtgg ctaacataca
    10021 atatattcat gtattttaaa tatgtaaaaa ccatctgatg aaataacgaa aaatagccat
    10081 tcaaaaaaaa catagagcat gctgaaaaat atcaataatc atctaacgta gcatcgaaac
    10141 aaaatattag tgcattaata aaaaatgaat ttccccaata tttccggaaa caaattttaa
    10201 ccccgatagt acttttcatt gcaccaaacc tgactgatat gtgccatccg gctgacactg
    10261 atttaccggc tattcacgca cccgcctgca gtcgggcaaa taataccaaa acgattgata
    10321 aatcggtaca atccattttt ggaagactcc ggaggtctca ttgaataaac cattccgtac
    10381 ctgacggcac gagctggcgt tctttttcgt gctaccaata ttttgtccct acgcgacaat
    10441 tgcagcttag tcttctgttt cccttggggc tccgaatttc gagaaaatcg cgccgctctt
    10501 tctgagctga aataacgcca aagcttttta caaatcggta caatccattt ttggaagact
    10561 ccggaggtct cattgaataa accattccgt acctgacggc acgagctggc gttctttttc
    10621 gtgctaccaa tattttgtcc ctacgcgaca attgcagctt agtcttctgt ttcccttggg
    10681 gctccgaatt tcgagaaaat cgcgccgctc tttctgagct gaaataacgc caaagctttt
    10741 tacaaatcgg tacaatccat ttttggaaga ctccggagga gtcacatatc tgtagataaa
    10801 atcaaacaaa accacgactc cggaggagtc gaatataaag agcatcatcc tctgataaag
    10861 cccgtatata tgcgcccacg ctgagcttgt gcttgcttag ccaataaaaa aacacaatgc
    10921 tgattatgct ttaccagcat aaagaaagat gatcgacaaa aaaatatcat caaacgtcgc
    10981 atcgtgatat ttaaaattat ttgtcttcaa aaaaatgaat tattacaaat acaatcagga
    11041 aaatcctatt tttgtttcaa agaacaaagc aatgattctt aagaaaatta ttttatcaat
    11101 actggtgctt ctaataacaa cacagctatt tgcaggcacc ggtaaagtcg attttatcga
    11161 atgcacagtc gatctgcaac cctccggcaa agccgtaatt ggttattgcg tgcgctacac
    11221 tgttgttgaa ggcgaattac atggttttta cttcagtgga ctcgaccgac ttgaaattga
    11281 atccttttcc gataagaatt atgccattga caaaaacggc aaccgcttcg atctgtcaat
    11341 aagcaatgtc ggcgggaaaa aatacgatat tgtgctggcc aatggaaaag gcatcgggag
    11401 cggatatgtt acttattttt tctggtttga aactaatttt tcgtcggccg gataccttgg
    11461 aacaaccacc agcgaaaacg gaaaaaatct tgtttatttc gactgggccc ccaccgaatg
    11521 ggattccgat gccacaggca tgcatcatta cactttgaaa attctgactc ctttcaaacc
    11581 atctttttca ttcaatgtcc gcgaacgggt ttatgctgat agtttgatta tgacagagaa
    11641 atggatgaat gaaaaatacg aaatcgattt tcagataaaa gaagccggac gcctgatgat
    11701 gctggtaaac aaaaatactc ctgaatatca tttctcgcca agggttcaga tttatatgcc
    11761 ggccgcatgg ttcgatacct ccatggtaag agagtatcag attagaactg atgaaacaag
    11821 caatggcaca caaaaacaaa gcaatgatag ttacaattat cagcaacccc agcaaactct
    11881 caaccctaca acactgggaa tccttgcagc agtactgctt ttaatatttg tactgtattt
    11941 tgttctcaaa aacaaagggc ttgcaaatgc ccgcgaaaat gcagataata ttggctggga
    12001 caagctcaac tggatacccc caaaacttga aatggccacc tactccattc ccggaaaaat
    12061 ctgcgacaat ctcaattatg ttgaaattgc cttttatctt gaatttccca tcaaaaagat
    12121 tttcagttcc atgcttgctg cattcgaggc ccaacaatat ataacagttt tgaaaaacga
    12181 tcctctcact ataaaaatca tcgaaatgcc cacatcgttt gacaatgatt atgaagaatt
    12241 gttttttaat gcactgactg atgacggcgt tttttcgcag gaagaattga atgccatcat
    12301 gaacaaagcc attgcaaaca tcagagaaaa ggcctgggat gcggatgtag atgcaacaaa
    12361 aaaatactac agtgagttga ttcaatcaaa aacagatgat tatgaaaagg caaatccatc
    12421 gcaaaaaatg catgttcagg acaataccga cctgatgtat aattactgga cccgctacta
    12481 tcattactat catccatatt tcacctatca gagtattatg aatccctcgc ggtacgagca
    12541 gtatttcccg gtcaataccg atcatgtggt ttacaacagt attcattctg acattattcc
    12601 cgacatcaat gcatgtcatg ccgcatgtca cgatgcttgt cacagtgcat gccacagcgc
    12661 ctgtcattcg gcttgccaca gtgcatgtca ttcggcttgt cacagcgcat gtgtcagtgg
    12721 gggctcacat taaatctgaa aaaaatggat caacgtttca attgggtaaa agaattcatt
    12781 gcaagtgtca aaccgtatat tgaagtacgg cttgaagata atatgctgat taaaaagccc
    12841 aacaatgttc agaaactaaa tccttcaggt gcattgatac tgtaccaatt gctgaatggg
    12901 agcagcattg atggattaat ccgaaaaatc ggaaacgatg aaaaacgttt gaatgaactt
    12961 ctttcatttt tactggctgt aaaatcatcc cttgaaggaa atcttgatgt atggacaaag
    13021 aacacggccg ttgtcaaggc cgcatttgaa gcgcctttca ccaaactacc ggttttaagc
    13081 gaactggctg taacaggaaa atgcaatctg cgttgttcgt tttgctatgc cggaatcaac
    13141 tacggatgca caactgaaac aactgatctg cgtgttgatc aactgaaaca attgatatat
    13201 aaaatccggc atcagggcaa agttccttcg atcagtttta ccggaggtga accaacttta
    13261 cgggccgaac taccagagct gattgcttat gcaaaaagtt tagacatgcg tgtaaacctc
    13321 atcagcaatg ggttcctgat agatgaagct atggctctcg gctttaaaaa tgctggtctc
    13381 gactcagcac aaatcagtct ggaagggatt actcaggaaa cacatgaaaa aataaccgga
    13441 gttggaggaa gttttgaaaa tgcactgaat gctgtttcag cactgaatgc acaaaaaatc
    13501 cgcactcata caaacactac attgtcaaag caaaatatgc acgaagttga cgggcttcca
    13561 cgttttctga agcatcattt caatgccgaa cgtttcagca tgaacctgat tatacccacc
    13621 ggaagcgcgc tggcaaacga aatgcatcta ctgcaatact ccgaagcagg ccatgttatt
    13681 cgacgtgttt ataacgcttc acttttagaa aacgttgaat tcatgtggta ttcacccact
    13741 cctttatgta ttttcaatac catcaccgaa ggacttggca acaaaggttg tgctgcctgc
    13801 gatggattgc tgtcggttga tccggaaggg aatattctgc catgctcatc gtggaacgaa
    13861 cccttgggaa atctgattac acaagacttt gaatccatct ggttttcgaa gagggctact
    13921 gaaatcagaa ataaaaaatc ggcgcccgaa atctgtaacg gatgcgacag tctggctatt
    13981 tgtcagggcg catgcccttt gttctacaga gccgaaagca atcatgaccg tgagctgatg
    14041 aaagcacgga tatcaaacat aacacacact gttttatgaa aaaactattc ttccattttt
    14101 ttccgcgcat gtcaaactca cttcgggtga ttgtttttat ttcgctcttt gctgccggaa
    14161 tagctattca gctgacaatt gcaaacttct ggattggcgc cattcttatt gcttgcgctt
    14221 cgctgatttt actcaataaa ggaattgatt cccggctgca tattgaacgt taccaccaca
    14281 atacggaatg gaaaacttcg acccgtcaac aagttgaaga tattattaaa atggataaaa
    14341 aactgagacg atgggaccgt actggttttg aggtcacttc atgtctgggc agttttctct
    14401 ttcttgttgt gcttgttgct ggctttttta ttgttattct tggtctcgaa gaaaatgacc
    14461 tgaatgtcat ctttttcggt ggcgattttc tgatactttt tattccgcat tttttatccg
    14521 gtttcagacg aatcgatatg gcttcaaggg tgctcttata tgccgataac tgcttgaaag
    14581 ccgcagatat tgccattgaa ctgaataaaa atgtgaaaac ggatttcatg actctgctgg
    14641 ctgaaaatgc aaaaacaaaa gcactttttc ctaaagaagt gaaactgaaa atgtcgcttg
    14701 aaaacgcccc ggatacattt cttggttgct acggacaatt atcggtgaac aaggttggct
    14761 caaaggatta tccctatttt tatacggttt tgatttttaa accggaattt gatctgaaga
    14821 aaaaattcaa taaactgaaa ctattaaaca aaggtatgtt gatagaattc acatctgaac
    14881 aaggtgttga agtgctggtg ctgcgacagg ttacaacccg caccagtggc tattctacaa
    14941 ataataaagc tgttcgggga attcttgtgc aaacgtttga tgcttatcag caactgatgt
    15001 cgatgtgatt ttttttgatt gaatatgcct gcagcagaca gccgtcaatc agagtctgct
    15061 gaagaaatct gagatacgac atactgcaac ttagcattac acttggattt atggcggcaa
    15121 aacaaagata tatctgaaga cttcaggtca aacgccacaa agaaaatggc gcacttcaga
    15181 ttattgaatt tcatattgat agctatccgg aatcgtaagt tcgacacgag ttccaagggc
    15241 ttcaccgttt tcatttttca gatcatggat aacaaaacca gccggattgt cattgtaacg
    15301 attcatgatt tcgaaaaatt cgctgaaaac cagcaaacca acacctgttg actgtgtgcc
    15361 atttttcgcc gatgcagccc tgccaacacc attgtcttcg acaatgaaaa cagttttatc
    15421 ctgttttttg gatattgaaa ttcgaagcaa tcctccgtgc atcaactgtt caattccgtg
    15481 tttcatggca ttttctacaa aaatctgcac acacatcttt ggtacttttt gcttgaatga
    15541 aaccaaagga tcaacctgta ttgaataatt gaaccgctct tcaaacctga atttctgcag
    15601 actcagataa ttttcgacaa acttcagttc ctcttcaata gtccggataa tcttatcggt
    15661 atctttaatt gaattgcgaa gcagctgcga gattttcaca aaataagaat aggccttgtc
    15721 gttgtttttt tcctgaactt cgctcccaat ggccgtaatg gtattgaaaa taaaatgcgg
    15781 atccaattgg gcattaatcg ttttaacctg catggcaacc aactctttct gtacttgtcg
    15841 ctcctgattt ttacgttttt gcttacgacg cataataaga tatacaattc cggcaactgc
    15901 caacaaatca aatattgcaa caccaattct aaaaccccat gtttgataaa atgccggttt
    15961 gatattgaat ttcaatatat gcggatcttt gctccagatg ccgtttccat tggcaaccgt
    16021 taattcaaac tgataaaagc caggacttaa attggtaaat attgcttcct tatcagtggt
    16081 ttcggtccag tattcactgt atccgattag ccggtatctg aatttcattt tttcaagact
    16141 tacatagtcg agaccgtcat acaaaatacg gatattgctt tcgccgggat caaggtgata
    16201 tactgcattt gtcggcttcc aggcaatact gatgtctttc caaagaagac ttttagaact
    16261 tcgctcaaaa gaaacaataa aaggcgtagg cggaaccgaa tcatacatgg ttttttcagg
    16321 atcaaagcgc acaacacttt ctgaggttgg tatccataca tgatttttcg aatctataca
    16381 tgttccattt tgcccacatt caattcccaa aaaaccgttg aaacgatcaa aatgctgcag
    16441 cgctgatgga ttctttgcat aaaacatatt caggtcaatc accgaaatac cctcaacacc
    16501 tccggcaata atttttttat tcttccaaag ggaaacaaaa aaataatgac cctcactaat
    16561 tttaatgtgg cctgcataat tgtacaaata caaaccatct ttacgggcat accacatatt
    16621 gcccaacgta tcttttttgc acgatatcac acccacattg ggcaaacccg aatgatctgc
    16681 tgtttgaaga atagagtcat ttttcaataa ataaacgagc ttgttggtgc atatccaata
    16741 gtcatcgtta cggtcctttt caatagccag tatgttgtat cctttggtgt caacatgttt
    16801 caactttccg ctcaaaagat tgtatataaa caacccattg gtggtgccaa aatatacatt
    16861 cttttcaatg gtgtcttcga atgttgccaa gcatgtgctg aagttacctt catgtaataa
    16921 aggaagaaaa cggtactgtc cgtgatcgtt gatgaatatt ctgtaattgg ttggaatcat
    16981 ccactgtccg agcgaattgc agaatccacc catgtaaaag acctcgttga tatccatatt
    17041 tttacgataa ccctgcactt cagacatgat attattgcgc aactgaaaca aacgaccatg
    17101 aaaagttgca aaaacaagtg tcgagtcttt gtcttcaaaa atactccaga cataacgcgg
    17161 catcgcagac ttccaatcgt aattgcgaaa tgcatatgac tgctgatgca acaatccgtt
    17221 ttcggtgccg ataaataaat ttccattgca atcctcaaaa atatcattga tgatcatatc
    17281 cggaatggaa tcgcggtcaa gaatatgtcc gctggtatcc agtcgaatcc agacagaact
    17341 gttctgatta atcagcaatt gattgttttt ggttttcaac atcacagcgc caggaatatt
    17401 atttccataa gcatatattt ttcgtggctg attttcatca aaacaatata tcccatcaaa
    17461 ctctgaaggc aatttgttgt ttctcactac tccaacaaaa agcttattgt caaccacaac
    17521 aatctgattg ccctgccctg taaaaacagg agtcagctgc ttgttacaat ccaaacgata
    17581 aacgcctttt ccggttgata ataaaaaatc acaacttccg ggaatccgct ccatatcggc
    17641 aataaagccc atgtcgtgat ttttataata gaaattttca cgccccggat gaatgacgca
    17701 cagcgcagta tctgaaagaa aataaatttc acgcaatcct gcattgacaa taacggtatg
    17761 aaccccaaat ccattaaaat ccttgatgag agatgattgt ttgttctcaa aacgcaccaa
    17821 tgcttcgcgc gtataataaa aaaactggtc tcctatttca aaaaatccct cgacaaagga
    17881 ttttgtaata taccggtcat tatatgttct gaatctgctt ccatcaaaac caccaatctc
    17941 gttttttgtt ccgtagagaa atattccatc ggagcgctga aatacattca caacctgatt
    18001 ttggggaagt ccatcaaata tggtgtaatg acgatagctg tattgctgcg caaaacctga
    18061 aaaaaatatg acttgtaata ttataaataa tcttgctttc atgatatgca ggttcaaata
    18121 taatcaatat ctgtttactg tcgtattttt cgaatttcaa agtcagggcc aacgcattaa
    18181 aacatgcaaa aagcatcttg aatcgcagtt tattcgctca ttaaattgat tttaaaggtg
    18241 ctcccttcgt caagctcaga acgtcgtatg agctcagcgc atttcatgca cttcggttca
    18301 cagagtatcg aaaaaacaaa agccgattag aaatgacaca acgtagttgt gtaaatcaat
    18361 tacaacatac cgaattctga aagctcgctt tcaaattcac tttgctgtaa ttgatatcgg
    18421 cttttgcctg attaaattta ctctgtgcga ctcagtgtgt actccgttaa actctgtgtc
    18481 acaaaggtag atgttgcatg cttgaagtca ccattgggtg catcaatcag aagtattcgc
    18541 atgcaattaa atgcgttgac cctgatttca aagtatcgaa gtccgtacaa aacacggtat
    18601 tcagccataa aacctatgca aatacaaaac gtgagccagt gaaagaatga aattcaactc
    18661 agaactatcg attacaaact ttgaaaacct gataaaacaa tctgcatgag gcctttgcag
    18721 ctacattaaa aatcataaac cagattattc agacttgttc ggaaaccgaa atagaaaaag
    18781 gtgctttctc tccaaccaat ttccggatca aaggttcggc gcatcaatcc cagcgtaaca
    18841 ttcatattcg tggctggatt caccagatag ctgacgctgg catcaacaat attcacagtg
    18901 gttttatcgc cctgcccgac gtaattaccg tattcaaacg ggcgcgtggt atatgattgc
    18961 cagatatcgt tgccgtagtt gatgccatca tcgtcggcgc caaacctgat aaaattatat
    19021 ttcagctgcg cataaaagcg cttgtaatga tatttcagaa tacttacgct ttcaataaaa
    19081 tttgcaccca gcggatgcgc cagtgcttcg ttgtaatgtc cgtaacacaa agtgctggtg
    19141 cggcttgaat atgtgtaagg ccgtacaaaa ttcacttcgc tttgcaacga aagtccatcc
    19201 aggcctgcta tgtcatatgt cctgaatccg gcctgcagcg ccgatttgtt gccccaccag
    19261 ccatcgcctg aaagtatttc atgcaccttg atttcatcca aaagaaactg tgcatacaac
    19321 acatttttct ttcgcagttt atacgatgca ttcaatccga ttaacacgtt gtcgggcgag
    19381 ccaagcgaaa attcaacagg ccggaaaaaa ataaccggat tcagataatt cacatcgaag
    19441 ccacgatggc cggtgctgtc ttcgttgatc cagatcaccg cctcataaag accaagctgc
    19501 aggcgcctat tcacagcgta attcagaaaa tgaaacgttc cgtatttttt ctgaaaacca
    19561 atgttgccac catgcggtac tttcaaatcc tgaaactgcg catacatgat gctgtacttc
    19621 agtttccacg cttctacatt gagtttcaga tagggataat tgaatgcata atcggacaac
    19681 agcaatgaac gatacccatc gccgtaaaag ttttttccgt gacccgcctg aatattcaga
    19741 aacttagacg gcgcccagga aatatgtccg ctggaatatc cgtaatcaaa accgctgctg
    19801 taaaaactgc gcgccatgcc ctgccccggc actacacgcg tgtttcggat gcgttcatct
    19861 atataatcaa caaaggttgc ctggttttcg taaaaagaag tgaagaacga aaactttttc
    19921 ccaatgctgc cttgtgcctg cacgccgcgc gtattgacaa aacgcaggtc ttcaattgca
    19981 gaaaaatctt ttccgggttc aaacgcaaaa accggatcaa tatcgagata aaaatcatcg
    20041 gtgcggactt gaaaaaaatg ctcattgaat gccttgcgga acaaccagct gccaaatttc
    20101 cgctgtgctg gattgctgaa cagttgagcg ctgcgtgatg aatccagatc aaaaactttt
    20161 ctcacatcag cttcaacaaa tggttttatg ctggtgtgaa aagatgtatc aggtgaatag
    20221 attgcttctt cgatatctgc gctgaaagct gtgttcatgg gtaacatgag ttgctgtgcc
    20281 tgtgcagaaa aacagaccgg caaaaatgca aataatatta ttgctttgaa taagctcatc
    20341 aaaaaatatt gtcgcaaaga taatgagttc agagaaatac cgctgggctg aaatttctta
    20401 ctcagcaaca cggctttccg gtttcaacag agcgaggttt accagtaact tttgcagaaa
    20461 gccggcgagc ccattttgta acaggaataa gataccacgc cgttgcaatg atggttaaaa
    20521 caaacaacag ctgcaaaatt ccgatatgct gcaaaaggaa tgataaaaca ataaaaacga
    20581 cactggtgaa tgaaatcatt aaagtggcct gcggatgcgt gtatccgaga tcgagcagct
    20641 tgtggtggag atgaccgcga tcggctttaa aaggagactt tctgcgcata atgcgagccg
    20701 acattacccg gatggtatcg taaatcggaa taaataatat gccaatagcc acagcaggtg
    20761 cagagcttac atgatgttca ataagaggag agcggtttaa ttcaataaag cgtatcgttg
    20821 caatggctat cacaaaacca ataagcaagg agccggtatc gcccatgaaa attttgtttt
    20881 taccattgga caggttatat ctcaaaaagc caatgagaga accggcaagc gaaaagcaca
    20941 tgatggcata gacataatcg ccggccaatg caaaccacgt cccaaaagta actgctgcaa
    21001 tatttccaag cgaaccagcc agcccatcaa tgccatcaca taaattaaaa gcattgataa
    21061 tggcaatgac gacaaaaaat gaaaatacaa tactggccca ataaggaatt tcattgatgc
    21121 caaacaatcc ctgaaaatca ctgatataaa tatttccaag aaaaacaacc agcaatgtgg
    21181 caaatatctg cgcaatgaat tttttgattg gagccaacac aaatatatca tctttcatac
    21241 ccatgagaaa aatgacaaga caacccgctg ctataaacgg acgttcgggc aacgcagtct
    21301 gaccagaaaa aacaaggtat gaaaatttaa aaccggcaaa aatggccaga ccgcccaggg
    21361 tgggcgttgc aattttatgt accgatcttt cgttgggttc ctcataaagt tttttcgcat
    21421 tggccaatga cacaatggcc gggatggtgg accaggtggc aattaaggca atggaaaatg
    21481 aaataatcag catcaaataa agaggcactg aataaatgtc gaattcgact gaattcatta
    21541 atgtactata catcggctac tacttattaa aagaaattct tgttctgtaa tactttactt
    21601 acttgaaagc gtgcaaaagt aatacttttt cgtctgcaaa aaaaataatt tttccccaaa
    21661 tgtgaatgta catcagatat acacatatat tgatgattta tatttatagt tataatggca
    21721 tgtttaagag acgaaaataa ctataatcaa tatatgcagc tgctttctat acataataac
    21781 agctgaattc cgttattcga aaatgtaaaa tttcaacatg aacgaatgaa tatttcataa
    21841 ataatccgtg cattacatat tccttgattc gacctctacg aggtcgctgt tatcatattt
    21901 cctctcaccc ggcattgcat gccgggctat ttttgtttga cctctacgag gtccgcaact
    21961 caaccctttc ctttcggcca ctacgaggtc aaggccagat cctcattgcg cggcgaatga
    22021 aatttctcac ccgcaggccg tagctcctga tgcggccgcg caaacgatcc gtggagcgga
    22081 tcgaacaaaa ataaccctcg gcgcagccgg gggtaaaaga cacacactcc tctccccgac
    22141 ccgcctcggg tgggtcgaat ccacaccacc attcgacctc tgcgacgtca agattatatc
    22201 cgcattaagc ggcgaagaac cgtttttgta tgatgacccg gaaactttat gagaagaaca
    22261 aattacatat attttttgat ggaaataaca gcttcaatca ttctcaaacc cctataaatt
    22321 cacaactaat ttaattgtct acatatgata cgtatggaca tatataactg tttgtcagct
    22381 atttattaat taacattctg tacatttgct ttcttgatat cataaaagaa taattatcat
    22441 taaagtgatt tccggaaatg aataattacg caaaaataaa atctcttgtt gccgctatcc
    22501 gaaaaatagc ccggaatgct gcatacagca cgtccaccat tcttctgatt gtaatatcat
    22561 ctgcattgca cgctgcagat ataaatacag ccggaacagg caactggagc agtcgtagct
    22621 catggagtca ggtggccggc accggtacaa tatcctacac ggccggatca accgatgtaa
    22681 ccggcacagg gactgcgttt acaactgaac ttaccgttgg cgacattatt tacacaaccc
    22741 aaccaaatgg attgtccatt gcaattgcaa gcattaccga caacaatcac ctgactttgg
    22801 tctcagctcc ctataccagt tctggacagc gaagctacac agtaagccga acacccgttg
    22861 ccagcgatga tgttcatatt attggaggat atacaattac tgttgacatc tcaaatgctt
    22921 attgctcctc gatttcaata ggaaacggag gaaataaaga atgcacactg gcatttaatg
    22981 ctaattctca actgtcggta agctcttctg taactctgaa tacaacgaag aataataaaa
    23041 ccattttgct cgatatgacc aacggcggca cactcatctg tgcatcgctt gctgtgccgt
    23101 ctacaggtgg tattcaaaaa ttcactgctg gcagcggcac tataattctg accgcaggca
    23161 acaccattcc agccgcggtt tttaccacat tcaacaacct gactttgcag gccggaacca
    23221 c
//
LOCUS       DAOPKE010000042        23147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2337_length_23147_cov_10.775117, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000042 DAOPKE010000000
VERSION     DAOPKE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_23147_cov_10.775117"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PKY63_06995"
     CDS             <1..1625
                     /locus_tag="PKY63_06995"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00395.1"
                     /translation="GNVVNALKVDITLQPGESKDIVFTLGVIEKTKFDALKKERSEKY
                     HSVSAVDAAFEKMNAAWEQYFAHTSAETPDKNLNIFLKYWMPYQAITAFNVGRVASFY
                     YWGIGRGFGFRDTAQDTIAVTISNSEKAKERILLLSRQMRTDGKVYHHFHGDGEGEFT
                     QHCDDPLWYMLAVTEYIKESGDLDILENVQPFIEAGKEGTVLEHMFAVVQYAKNNLGV
                     HGLPVFGRGDWNDTLDYIGGDEGGESVWGGMFYAAMLKLFMDLLEHIGMTKEREDVSF
                     VRKKLMASIEEHCWDGEWYIRAFGGKGNKVGSKENYYGKIFLNTQIWPVLAGFKNHNR
                     LIMAMDSVKKHLDSPEGPKKCTPAWKEIDPNIGLVTRCVWGKKENGAVFCHPTSWVIQ
                     AETMLQRGNQAYEYLKKMLPDRIDSDVFVAEPYVFSQYITSNEHSEPGRASHSWQTGS
                     AAWMFRVSFDYVLGIRPTYNGLMIDPVIPSHWDTFTASRKFRGTQYNITVENPSHVEC
                     GVKEVHINGSRIDGNVLPVSETEVCNVKVIMG"
     gene            1627..2586
                     /locus_tag="PKY63_07000"
     CDS             1627..2586
                     /locus_tag="PKY63_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPF00396.1"
                     /translation="MKRHPQNPILTRENIPDIHPQLVDATSVFNPGAIYFKGKYLLML
                     RVQSRSRETFMVMAESPDGINFSVENKIVHFRGIENIKEKIYHIYDARISNIDGQYYI
                     MFAMDMDGGCQLGLGTTKDFKEFDFIGIVSDDDIRNGVLFPEKINGKFMRMDRPNKAR
                     HSNGTTSGSVIWISESDDMLHWQPVAPLIEGRFHYWDEFIGSGPPPVKTRQGWLHIYH
                     GVAGHFGSSNIYQAGVMLLDLNNPAKVLSRSWCNILEPRELYELTGQVPNVVFPCGII
                     VKEYDSDGFAKDDSEVFVYYGAADTCVGLAVSTIHELISLTQQ"
     gene            2593..4524
                     /locus_tag="PKY63_07005"
     CDS             2593..4524
                     /locus_tag="PKY63_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00397.1"
                     /translation="MNLSTIDWSIVLIFLALMIAMVLFSKNLVKSVSDFLATGRTGGR
                     YIISMFHGTAALGAITVIGALEQNYSAGFNSRWWEMPTAVILVAISVTGWVVYRFRRT
                     KALTMAQFFEMRYSRAFRIFAGFLAFLSGVFNMIIFPSVSARFFIYFCGIPEIGEVFG
                     ISLTFVAVTGAMVLIPLYFIFTGGHIAVMFTDFIQGVFVNIVFVVVVILLLVQVDWGH
                     ISEAVNSAPSGQSLINPFDADKVQDFNPWYFFIGMFGLIYTKLSWQGNSSFNIAAKSA
                     HEARMADVLTNWRLVPQWGLFLVFVPVVAYTIFNHADFNSVAASINATLTNVGTVAEQ
                     SQLRVPMALNYLLPIGLKGAFVAIMLAAAVTCHNTYMHSWGSIFVQDCIMPLRKKPFE
                     PKQHLKYLKLSILGVGITIFFLSILFQQPDKVFLFLNVSGAIFVGGSGAVIIGGLYWK
                     RGTTAAAWAAMITGALTATINIVLKSVIEDYPINGQWGWFMAMIAASVVYIVVSLLTP
                     KPKLNMDKLLHRGEFSDHAKEKTGGETPSKGWKVMAWTKEFTRGDKIIYAVTIGWTLL
                     WVLVFIAGTIYNFVVEPIENSTWMQFWKIYTYIFLGVSIIVTIWFTIGGIKNLREMIS
                     ALRHNLRDHSDDGFVKKEE"
     gene            4765..5808
                     /locus_tag="PKY63_07010"
     CDS             4765..5808
                     /locus_tag="PKY63_07010"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPF00398.1"
                     /translation="MKPLQDDLKALMQEPKSYVCYRSDGALSIDGMPDEQSWNKAEWT
                     DLFVDIEGDAKPKPAQNTRVKMLWDNDYLYILAELEETNIWAYLEKHDEVVYYDNDFE
                     IFIDPDNDAKNYFEYEVNAKGTVFDLFLPHPYRHSSFALHNWDFKGIKKAIKIDGTLN
                     DGSDKDLKWTVELAIPFTDLSFGLDSGKPNVDTPWRINFSRVEWDTKWENGKYVKATN
                     ADGKPLPEHNWVWSPVGAISMHMPERYGYLKFSEKMVGSDKETFSLSETEKLKSVLWA
                     VFYREKKYSEKKKKFSDKISDLGSDIKSLYDNKKYKLSISASDHTFEATLISSDKKMK
                     LFITQEGTVVRSL"
     gene            complement(5845..6579)
                     /locus_tag="PKY63_07015"
     CDS             complement(5845..6579)
                     /locus_tag="PKY63_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPF00399.1"
                     /translation="MKNYRLEVVVTSLESLESAIEGGADRIELCSNLFCGGTTPSAGL
                     IQAAISESSVPVHVLIRPRSGDFVYSKSEMNEICRDIEFAKQSGAAGIVCGFLLPDGN
                     IDKEITELIVELASPLSVTFHRAFDVCRDPLQGLEDIIEAGCQRLLTSGQKNTAFEGR
                     DLISQLVKQAGDRIIIMPGSGVNADNIVQLKETGAHEFHMSGSVNRDSASTYNHDKVL
                     LSDNPIRDSVIQMTSKEKVAAVKRLL"
     gene            complement(6582..9212)
                     /locus_tag="PKY63_07020"
     CDS             complement(6582..9212)
                     /locus_tag="PKY63_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPF00400.1"
                     /translation="MKSLLLFVAVWGITIGAAPLFSQVLMKDKVAYENMNKLFSDRKA
                     TFGARLPDLFAVVEKAETPELAEATKFLIAYAPLSDIANLSPEYFKSMAQLALRTRSE
                     FSWGKSIPAEVFMHFVLPFRVNNENPDEARAVFLPELKERVQGMNMYDAALEVNHWCH
                     EKVAYRSTDERTSSPLATIKTAYGRCGEESTFTVAAMRSIGIPARQVYTPRWAHTDDN
                     HAWVEVWVDGKWYFMGACEPDPELNMGWFSGPATRAIMVHTNTFGQYNGPEKTLVQKP
                     LYSKLNLLSNYAPSKQAVVKVVDAQGKPIEGIMVDFQVYNYSEFFTFASLKTGNDGRC
                     SIETAFGDLMIWVNDGKDFTWKLFPSEQESVLEIIFDPYEIQQDSSVFAFQILPPSLG
                     KTVTPTTSKKEENDRRLKEEDVIRAAYEVTFPDTVSLREQYKNDRYGEAMVNFILKSR
                     GNYSEIASFYNRADNKETAAELLSVISEKDLRDTRADILLNHLMNAERFSCNNCPEDI
                     YTKYVLSPRIGRELLSSWRSELQNSFSKKEIKSFKKNPENLMAWINSNIKLDTENNYY
                     GVTLFPGSVMKLRAADAYSRNVFFVACCRSFGIPARLEPATYIPQYYSKDKWISVFRS
                     AAAPDVEKVPVHIFNVGFTPKYYTSYTIARLIDGRFQTLDYEYDESMDVFPVNMQLEP
                     GVYRIMTGNRLPDGSVLTNWSFLHIPEKSDTVRIRLYQQESAVFPESLFQFKGDFEMK
                     YLNKDSVISFNAISNSSSNIIALIDPSTEPGKHFLTELTQSKTDFDKLNRNIFLVLPK
                     EKPAASFDMKLWPGLPDNVVWLSDLKGQFAEQLKISSGKELSYPEVLFISENAVYYHF
                     SGYHINTAAMLLNAYNRKIF"
     gene            complement(9222..11477)
                     /locus_tag="PKY63_07025"
     CDS             complement(9222..11477)
                     /locus_tag="PKY63_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPF00401.1"
                     /translation="MKKFGFFVGFFSLITVLSAQNQNVNLIPKPLSISVGSGVFNFDA
                     SVRMLAETSPGILRTSHLFVNQLFTSTGVALQFDSVRHKNIRYISVLRDDSAKWCGSE
                     GYHLVVSGNEIKIIAMTETGIFYAFQTLRQMMPVGFGYEKNIDIASIKLPCAKISDKP
                     RFSWRGMNLDCCRHFMDVEFVKRYIDLLAYHKMNVFHWHLTEDQGWRIEIKKYPELTK
                     VGAWRKEDDGSVYGGFYTQDQIREVVAYAAERYITVIPEIEMPGHSVAALAAYPQLSC
                     TGGPFEVEKRWGVFNDIYCAGNDSVFIFLQDVLTEVMDLFPSKYIHIGGDEAPKVRWE
                     ACPKCQARMKSENIKSEEELQGYFNQRIEKFLNAHGRQLIGWDEILEGGLAPSATVQS
                     WRGMEGGMEAAMQGHDAIMSPTSHCYFDYSTTSTNLEHVYSFEPVPADLPADKRHHII
                     GVECCMWTETAPQEVIDSRVFPRLCALCEVMWSDPTGRNFKEFHDRLAVHYKRLDQMN
                     VQYGYERDPIKLTTSFDKAQNAFVAVVEHGQPGLTMYYTTDGSDPDFNSVKYTVPVVM
                     KTSAEIKVAAMREGSGKTAVESRKVCWHKALGKEYTITYPFHKKYVAGGDTNMTDGLR
                     GTYSYRDNLWQGYEGIDFEAIVNLGAKTKISKVSVGSLQSTGAWIYHPLKLEVFVSAD
                     AADWKKVGEVENNVPQTDAATSVQDLMVSFKVQKVKFVKVYCSAMRQNPDWHPGAGGK
                     TWLFLDEIIVE"
     gene            complement(11608..12180)
                     /locus_tag="PKY63_07030"
     CDS             complement(11608..12180)
                     /locus_tag="PKY63_07030"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPF00402.1"
                     /translation="MKVLFIIVAVLLISCSGDEKKEETRYINYTLPLSQVLDEKNIDK
                     KDIQIVISKSKYTLYVKYNQEILKSYPVVLGPDPVNDKRMEGDGCTPEGVFGIRDKYP
                     HRNWTRFIWLDYPNKDSWKKFKRAKDEGEIPQDATIGGEVGIHGVPEGKDQWIDQHKN
                     WTLGCISLKNADIVEIYDVINDDTKIRIHK"
     gene            12255..12863
                     /locus_tag="PKY63_07035"
     CDS             12255..12863
                     /locus_tag="PKY63_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPF00403.1"
                     /translation="MQTYKHNRLPFLETIRVVDGIIEYSDYHIERMRQTCLFHYHEFG
                     LEKILDNLRLPENGILKLNIWYNQHEKEIKISEYVPTSIKKIELIECDPDFNYSFKYS
                     DRSYLNNLLKSASGADEIIIVKNGMITDTSKANIVFEKNGKFYTPDTFLLNGTMRQYL
                     LGSGKITEASIYAENIFDYEKMYFINALNPLGKTPVFNGRIL"
     gene            12962..20779
                     /locus_tag="PKY63_07040"
     CDS             12962..20779
                     /locus_tag="PKY63_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00404.1"
                     /translation="MKKLFTLIAFVFSCTILSAQSITVTSPNGGEVLPGCTIKTITWT
                     SSGTTNYYSIDYSTDNGNTWTSITSFYNTVSGTYNWTVPNTSSGICLIRIVDSNNSSI
                     FDISDAVFTVTSPLTVNAPNGGESWQAGTSKSIQFNASGTSNYYDIYYSTNAGANWTS
                     VATNTYNVSGTYNWNVPNTPSTNCLVKISDHSNTCMTDISNGLFTITAAASSVTVTSP
                     NGGETWYVGQSKNITWTASNTTNYYNIDYSTDNGTTWTSLASSYYAVTGTFPWTVPNT
                     PSTNCIVRVTDANNLPVTDQSNAVFTIAAPFITVVSPNGGESYESCYGQYINFQCGGT
                     SSYYKIEYSTNNGTSWTTINSSYYATGSNVSFLWSTIPQNSSANCLVRVTDANKATVL
                     DQSDATFTIVPNVEIIVTSPNGGESWEVGTSRIITWVAAPTSTRFNVYYSVNSGASWT
                     YINNTYSTSLSWTVPDNESTTALIKVVDYNNSCINDVSNTVFTIAPPTPVITVTSPNT
                     NVTWYQGNSNTISWTSQYVESPFVTIEYSSDNGSSWNTIAAVTENDGSYSWLIPTVVS
                     SQCLVRVSEYLNPVVFDVSNTSFNIAAPFITVTSPNGGNIFGGCDAHTITYTKGGISS
                     NVKIEYSPDNGATWVNIVSSTSASGSYNWSIVANIASTQCLIRVSDAANATVNDVSNA
                     VFTIEKNDDIFVTSPNGGESFEVGAQYTVSWAAASGHSQFSVYYSYDNGTSWSTLYSS
                     IYSTSFSWTVPNTPSANCLIKVVDYNNSCIYDVSNANFSIEPPQAIITVTSPNTAQTL
                     YVANSHTITWTHQYDDADFVGIDYSYDNGATWINVVSVTENDGSYTWASIPNTPSTQC
                     LVRVYEYNNMSLIDQSNVNFTIAPPFIVLNQPNGGEVMTGCGTYNVTWTKGGTSSYYF
                     LDYSLNNGTTWNTINSSYYSTGTNNSFSWNPVVNQSSTTCLFRVRDANNLSTGDISQA
                     VFTITPNTNIIVTAPNGGETLEIGDTYQITWVSAGTSTQFRVYYSINNGASWTTLTSS
                     TYSNYYNWTIPNAPSTQCLVRVEDYNNSCINDVSNAVFTIAEPAPNITVTYPNTAVTL
                     YYNATATISWTSEFTVSSFVNIEYSIDNGASWTTIASPTEDDGSYNWTVPLTASTQCL
                     VRVSEYGNPAVFDVSNVNFTIAPPFVTVTYPNGGQTIEGCASANITWSKGGTSSYYKI
                     EYSIDNGSNWTVINSSYYTTGGSFTWSPVADYNSTQALVRVSDANYPLATDQSNAVFT
                     LLKNTDIVITNPNGAEQLEVGTTYQINWVADASVSRFNLYYSADGGASWTYIGNTYSH
                     YYSWTIPNNISAHCLLKVVDFDNTCRNDISDAEFSITPPTPVIIVTAPNTATTLYFTK
                     TATISWTSQYLVSPFVSIEYTYDNGLTWHSIANATENDGSYSWTIPETPSTQCKVRIS
                     EYQNPVVFDMSDVNFSIAYPYIIVTAPNGGEVYEGCETATINWTRAGVSNNYKIEYSS
                     NGGVNWTIINSSYNTSGSSYSWTPLPDLTSTNCMVRISDANYPAATDMSNAAFTISKN
                     EDIIVTSPNGGEFWQVGTTKTITWVAAPTSTQFRVYYSVDGGTSYTQINTTYSTSLSW
                     TIPNIPSSSCKIKVVDYNNSCIYDESNDYFAIVPADVALTYPNGGQSLYYGSSYSITW
                     TNQYINSNFVKLEYSTNNGASWNVIASIANNTGSYSWSVPQDFSTQCLVRVSQYNDPN
                     VFDISDNVFSISPSIVLNTPNGDGGAEVWRVCTETSIVWSANGDGNYFVIEYSINNGL
                     SWNTIESNYYNTSSTISYNWTLPNLPSTQCLVRVTDKNTPVKTDVSDATFTISPAINL
                     VNPNGGESITGGTPYTINWTSAGVSNYYNIDYSSDGGTTWVNICYNQNITTNSYVWNV
                     PSLISSNCKLRVTDNVNTCKTDMSNQVFAIGTAAVNITVTTPSGGQTWAGCTTETIAW
                     TSTGTSNVYDIFYSTNGGASWILIADNYSTLAKTYDWTLPNLASANCRIKITDAGNSS
                     YYGTSAANFTITTVVANAGTDAAICAGNSLNLNASGGVSYAWLPTAGLSNAGIANPVA
                     TPSSTTTYTVYVTDINGCTQTDQITINVNPIPAAPVASSNSPVNLNGSIELTASTVNM
                     AFYSWTGPDGFTSTSQNPVIPNATATNSGTYSVIATVAGCASTAATTNVVVTGVPATV
                     EIAGNVLTETGSPVSGVTLTLSGDGTDTYVTSANGYYDFTLNNSGTYVVSPVKDNDIV
                     TNNGISTMDIVLMQRHILNVQSLPTAYSRIAADVNRSSGITTLDIVLTRSVILQTATE
                     FPNGDMWTFVNSDYVFPDVQAPWSYEMSRSYSAAAEAEDQDFIGIKLGDVNNSWNTNI
                     AKSTETELILKSGATQAQSGQTLSIPFTAQNFDQLSGLQMTIAWDTTVFDFNAATANI
                     PGLYFGENYTAGGKLTAIWSTGDMAGVSYAPNDILFTIDLIPVTDQHVITDISVCSDI
                     TSAEAYDAALIPMNIVLQNSSIELNPVITQIEDSGSGIQLNCYPNPFDDRIIIEFYNS
                     TDSKNTLQIFNNLGEIVYASTTDMQQDINRIEWDGSDMNGHKLPAGNYFLKLISDDAT
                     SSRKLVIVR"
     gene            20809..>23147
                     /locus_tag="PKY63_07045"
     CDS             20809..>23147
                     /locus_tag="PKY63_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HPF00405.1"
                     /translation="MKYFKIIFALLFLPLISQAQAVYFRLGSGSNIQGTEVVIPVTVQ
                     NFSNLISMQGTVSFNPAVISFVNTENYGVSDLSPANFGTTNAATGHISFSWNDPDLSG
                     VSLSDGDTIFCLRFLMIGSAGMSSVIEINDDITPIEVIDKFFAAKTVYTQSGAATVQA
                     GQAVSGLLLSVDETEGLSGQSVQVPVTAQDFTDLLSAQGSIAFDPAIISFTGISGYGL
                     PGMNASNFGISSAGSGIITFSWNDATLAGVSLIDGAEIFVLNFNIIGSAGQISPVQLT
                     NNPTQLEFADITYSVVTYSLSQGSVTVGTPFIPEDIFIFADSIEGAMGQYVQVPVRVS
                     DFSNIISAQGSLSFNPAIAQFDQIVSFGLADMTNANFGTTQAASGLISFSWNDPDLSG
                     ESLADSAIIFTLQFHLVGTIGQQTPLSFVNSPTPFEFVQNDFTVLNYSSAPGNIKIID
                     LYELEVASVSSTEICAGADLTIQYSAIGNYSAANVFTAFLSDASGSFALPVSIGSLTS
                     NVSGSINVTIPANTATGIHYRIRVDASDPYYVGDANATDITIHAIPNANAGSDQDICI
                     GNSVTLTATGGVSYAWNSGQNTGAITVSPALNTEYIVTVTDAFGCENSDTVNVVVHAL
                     PSANAGSDVNICFGESASLSASGGNAYAWNSGQNTADITVSPTATTQFIVSVTDIWSC
                     QAADTVVVNVFQVFANAGSDEDICSGGSTLLTATGGTSYAWSTGQNTDAITVSPVVSS
                     NYIVTVTGVNNCTDLDTVSVIVHAIPNANAGSDQDICIG"
BASE COUNT         6599 a         5393 c         4921 g         6234 t
ORIGIN      
        1 tcggaaatgt tgtcaacgca ttaaaagttg atatcacgct gcagccgggc gaatcgaagg
       61 atattgtttt cacactcggc gtgattgaaa aaacaaaatt cgatgcactg aaaaaagagc
      121 gctcggaaaa ataccattca gtttcggctg tagatgcggc tttcgaaaaa atgaatgcgg
      181 cgtgggagca gtatttcgcc catacctcgg cagaaacacc ggataaaaat ctgaacattt
      241 ttctgaaata ctggatgccc tatcaggcca tcactgcctt caacgtaggt cgtgtggcca
      301 gtttctacta ctggggcatc ggacgtggct tcggattccg cgacacagcg caggatacca
      361 ttgcggttac catttcaaat tcagaaaaag caaaggaaag aattctgctc ctttcgcgcc
      421 agatgcgcac cgacggaaaa gtgtaccatc attttcatgg cgatggtgag ggcgaattca
      481 cacagcattg cgatgatccg ctctggtaca tgcttgcagt gaccgagtac ataaaagaaa
      541 gtggtgacct cgacattctg gaaaacgtac aaccctttat tgaagccgga aaagaaggaa
      601 ctgttcttga acacatgttt gccgttgtgc agtatgcaaa aaacaatctc ggtgttcatg
      661 gcttacctgt ttttggtcgc ggagactgga acgacacact cgattatatc ggcggagatg
      721 aaggcggaga aagcgtttgg ggcggcatgt tctatgcagc catgcttaaa ttattcatgg
      781 atctgctcga acatatcggc atgacaaaag aacgcgaaga tgtttcgttt gttcgcaaaa
      841 aactgatggc ttcgatcgaa gaacactgct gggacggcga atggtatatt cgcgcctttg
      901 gcggcaaagg aaacaaagtc ggctcgaaag aaaactacta cggaaaaata tttcttaata
      961 cacagatctg gccggtactg gccggattca agaatcacaa ccggctcatc atggcgatgg
     1021 acagcgtgaa gaaacatctt gattcacccg aaggtccgaa aaaatgcacg ccggcatgga
     1081 aagaaatcga tccgaacatc ggactggtga cacgttgcgt gtggggtaaa aaagaaaacg
     1141 gcgcagtatt ttgtcacccg acatcgtggg ttatacaagc tgaaacaatg ttgcagcgcg
     1201 gaaatcaggc atatgaatat ctgaagaaaa tgttgccaga tcgcatcgac agtgatgtgt
     1261 tcgtggcaga accatacgtt ttctcgcaat acatcaccag caacgaacat tccgaacccg
     1321 gccgtgcaag ccattcctgg cagacaggtt cggccgcatg gatgtttcgc gtgagcttcg
     1381 attacgtgct tggaatccgg ccgacataca acgggttaat gattgaccct gtcattccgt
     1441 cgcactggga cacatttacg gcaagcagga aatttcgggg cacacaatat aatatcacgg
     1501 ttgaaaatcc ttcacatgtt gaatgtggtg taaaagaggt gcacatcaat ggaagcagaa
     1561 ttgatggaaa cgtgctgcca gtgagcgaaa ccgaagtttg caatgtaaaa gtaatcatgg
     1621 gataatatga aaagacatcc gcaaaatccg attctcacac gcgaaaatat tccggatatt
     1681 catccgcaac tggtggatgc aacttcggtt ttcaatccgg gtgcaatata tttcaaagga
     1741 aaatatttgc tcatgcttcg tgtacaaagc agatccagag agacttttat ggtaatggcc
     1801 gaaagtccgg atggaataaa tttttctgta gaaaataaaa tagttcattt cagaggcatt
     1861 gaaaacataa aggaaaaaat ctatcacatc tatgacgcac gcatttcaaa catcgatgga
     1921 caatattaca tcatgtttgc gatggacatg gatggtggct gccagcttgg gcttgggacc
     1981 acaaaagatt ttaaggaatt tgattttatc ggcattgttt ccgacgacga tatccgtaac
     2041 ggcgtattgt ttcctgaaaa aatcaacggg aaattcatgc gcatggacag gcccaacaag
     2101 gcgcggcaca gcaacggaac aacatcagga agcgttatct ggatatccga atcagatgat
     2161 atgctgcatt ggcaacccgt tgcaccgctg attgaaggcc gttttcacta ttgggatgaa
     2221 ttcatcggat cgggaccacc gccggtaaag acgcgccagg gatggcttca catctatcac
     2281 ggcgttgccg gacatttcgg aagctccaac atttatcagg caggtgtgat gttgcttgac
     2341 ctgaacaatc cggcaaaagt actgtcgcgc agctggtgca atatactcga accacgcgag
     2401 ctgtacgaac tcacgggcca ggtgccgaat gtggttttcc cctgcggtat aattgtaaag
     2461 gaatatgatt ccgacggttt tgcaaaagac gacagcgaag tgtttgttta ttacggtgcc
     2521 gccgatacct gtgtcgggct ggccgtttca acgattcatg aactgatttc cctcacccaa
     2581 caatagtaca gaatgaattt atcaaccata gactggtcca ttgttctcat cttccttgcg
     2641 ttgatgattg cgatggtatt gttcagtaaa aatctggtga aaagtgtttc cgatttcctc
     2701 gctacaggcc gcaccggcgg acggtatatt atttcgatgt ttcacggaac cgccgctttg
     2761 ggcgccatta ctgtgattgg tgcactcgaa caaaactaca gcgccggatt caattcgcgc
     2821 tggtgggaaa tgcctaccgc tgtaatcctg gtcgccatca gcgttacagg atgggttgtg
     2881 tatcgcttcc gtcggacaaa agctttaacg atggcgcagt ttttcgaaat gcgctacagc
     2941 agagcgtttc gcatctttgc cggatttctc gcttttctgt cgggcgtctt caatatgatt
     3001 attttccctt cggtgagtgc ccgctttttc atttatttct gcggcattcc cgaaatcggc
     3061 gaagtgtttg gaatatcgct caccttcgtt gccgtcaccg gcgctatggt gctcatcccg
     3121 ctatatttca tatttacagg cggtcacatc gcggttatgt tcactgattt tattcaaggt
     3181 gtttttgtaa acattgtttt tgttgtcgtt gtaatcctgc tgctggtgca ggttgactgg
     3241 ggtcacatat ccgaagcagt taattctgct ccatccggac aatcgctcat caatccgttc
     3301 gatgcagata aggtgcagga tttcaacccc tggtattttt tcattggtat gtttggattg
     3361 atctatacca aactgtcatg gcagggtaat tcatcgttca acatagcggc caaaagcgca
     3421 cacgaagcac ggatggccga tgtgctcacc aactggcggc tggtcccgca atggggattg
     3481 ttccttgttt ttgttccggt ggttgcctat accatattta atcatgcaga tttcaacagc
     3541 gttgcagcat cgattaatgc tacactcacg aatgtaggaa ctgttgccga acaaagtcag
     3601 ttacgtgtac caatggcgtt gaactatctt cttccgatag gactgaaagg cgcatttgtc
     3661 gccattatgc tggcagcagc cgtaacctgt cacaacactt acatgcactc atggggaagc
     3721 atttttgttc aggactgcat catgccgttg cgtaaaaaac cttttgaacc caaacagcat
     3781 ctcaaatatc tgaagctctc cattctcgga gtcggcatta ccattttctt tctgagcatt
     3841 ttatttcagc agcccgataa agtcttcttg tttttaaatg tatcgggtgc cattttcgtt
     3901 ggcggatcag gcgcagtcat cattggcgga ttgtattgga aacgcggcac tacagctgct
     3961 gcctgggctg ccatgattac cggcgcactc acggcgacca taaacatagt tctgaaatca
     4021 gtcattgaag attatccgat caatggacag tggggctggt ttatggccat gatagcggct
     4081 tcggtggtgt acatagtggt gtcattactt acgccaaaac ccaaattgaa tatggataag
     4141 ctgctgcacc gcggcgaatt ctccgatcat gcaaaagaaa aaaccggtgg cgaaacacct
     4201 tcgaaaggtt ggaaagtgat ggcctggact aaggaattta ctcggggcga taaaattatc
     4261 tatgccgtta ccatcggctg gacattgctc tgggtgctgg ttttcatagc agggaccatt
     4321 tacaattttg ttgttgagcc catcgaaaac agcacatgga tgcagttctg gaagatttac
     4381 acgtacatat tccttggcgt ttcgatcatt gtgaccatct ggttcaccat cggtggaatc
     4441 aaaaatctgc gtgaaatgat ttcggcgctg cgccacaatc tgcgcgatca cagcgatgat
     4501 gggtttgtga aaaaagaaga atgatggatt gctgtccgac agattccccg cggcaatagc
     4561 tccgttaaag tctgttctac tgtctatcct tcgactccgc tcaggatgac agatgcaggg
     4621 atcaggatga cagcagggta cagggaaaca aaaaagaaaa agaattaaat gaagttcggt
     4681 aaaatgaaaa tgaaattcaa tataatctta ctaatcaccg cggggatcct gctttcctgc
     4741 aatcattcac aagaatcaaa aaaaatgaaa ccactccagg acgatctgaa agctttgatg
     4801 caggaaccga aaagttatgt ctgttatcgc tctgacggag cattaagcat tgatggcatg
     4861 ccggatgaac aatcgtggaa caaagcggaa tggactgatt tgtttgttga cattgaaggc
     4921 gatgcaaaac caaaacccgc acagaacacc cgcgtgaaaa tgctttggga taatgactat
     4981 ctctacattc tggccgaact ggaagagaca aatatctggg catatctgga aaaacacgat
     5041 gaagttgtgt actatgacaa tgattttgaa atattcattg atccggataa tgatgcaaaa
     5101 aattatttcg aatacgaagt gaatgcaaaa ggaactgttt tcgatttatt tctcccccac
     5161 ccctaccggc attcgtcctt cgcgctccac aactgggatt tcaaaggaat caaaaaagca
     5221 ataaaaattg acggaaccct gaatgacgga tcagacaagg atttgaaatg gaccgtagaa
     5281 cttgccattc cattcaccga tttgtctttc ggccttgaca gcggcaaacc gaatgtcgat
     5341 acaccatggc gtattaattt ttcacgcgtc gaatgggata caaaatggga aaacggaaaa
     5401 tatgtgaagg ctacaaatgc tgatggaaag ccacttccgg agcacaactg ggtgtggtcg
     5461 cccgtgggcg ccatcagcat gcacatgcct gagcgctatg gctatctgaa attttcagaa
     5521 aaaatggtcg gttcagataa agaaacattc agtctttccg aaacagaaaa actgaaaagc
     5581 gtcctctggg cagtgtttta cagagaaaag aaatattctg agaaaaagaa aaaattctcg
     5641 gataagattt ctgatctcgg atccgatatc aaatcgcttt acgacaataa aaagtataaa
     5701 ctctcgattt cggcttcaga tcacacgttc gaagcaacgc tcatcagtag tgacaaaaaa
     5761 atgaagctgt ttattacgca ggaagggacg gtggtgcgaa gtttatgata cggtagtgcg
     5821 tactttataa acttcgcact acttttacaa taatcgcttc accgcagcca ctttttcttt
     5881 cgaagtcatc tgaatcacgc tgtcgcgaat tggattatcg ctcagcaaaa ctttatcatg
     5941 attataagta cttgccgaat cgcggttaac cgagccgctc atgtgaaatt catgcgcgcc
     6001 ggtttctttc agctgaacaa tattatctgc attcacaccg ctgccaggca taatgatgat
     6061 gcggtcgccg gcctgcttca ccaattgcga aatcagatca cgtccttcga atgcagtgtt
     6121 tttctgacca cttgtcagca ggcgctgaca gccggcttca ataatatctt cgagcccctg
     6181 caacggatct cggcacacat caaaagcacg gtgaaaagtc acactcagcg gtgaagcaag
     6241 ctccacgatc agctctgtaa tttctttgtc aatgtttcca tcaggcaaca gaaaaccgca
     6301 cacaatgccg gctgcaccac tttgtttggc gaattcaatg tcgcggcaaa tctcattcat
     6361 ctccgacttg ctgtaaacaa aatcaccaga ccgcgggcgg ataagaacat gcaccggcac
     6421 actgctttca ctgatggccg cctgaatcaa tccggcagaa ggggtcgtac cgccacagaa
     6481 taaattactg caaagctcaa tacggtcagc tccgccctca atggcagact caagcgattc
     6541 gagcgatgta actacaactt ccaaccgata gtttttcata actaaaatat ttttctgtta
     6601 tacgcattca acaacatagc agctgtattg atgtgatagc cgctgaaatg ataataaaca
     6661 gcgttttccg aaataaataa aacttccgga tatgataatt ctttgcccga ggaaattttc
     6721 aattgctcag caaactggcc ttttaaatcc gaaagccaga caacattgtc aggcaatccg
     6781 ggccacaatt tcatatcgaa tgaagctgct ggtttttctt ttggaagtac cagaaaaata
     6841 tttctgttca gcttgtcaaa atcagttttg ctttgtgtca actctgtcag aaagtgcttg
     6901 ccaggctcag ttgaggggtc tatcaaagca ataatgttac tggaactatt cgatattgca
     6961 ttgaaagaaa ttacagaatc tttattcaga tatttcattt caaaatcacc tttgaattga
     7021 aaaagagatt ccgggaaaac tgctgattcc tgctgataaa gtctgattcg gaccgtatca
     7081 cttttttcgg gaatatgcaa aaaactccag tttgtcagca cactcccgtc gggaagacgg
     7141 ttccctgtca taattctgta aacacctggc tccagttgca tgttaaccgg aaacacatcc
     7201 atgctttcgt catattcata atcaagtgtc tgaaaacggc catcaatcag gcgcgcaatg
     7261 gtataactgg tataatactt tggcgtaaaa ccaacattaa atatatgcac aggtaccttc
     7321 tcaacatcag gtgcagccgc acttctgaac accgatatcc atttgtcttt gctgtaatac
     7381 tgcggaatat aggttgcagg ttccaatcgc gcaggaattc cgaaactgcg gcaacaagcc
     7441 acaaaaaaca cattacgcga ataagcatcg gctgctcgca atttcataac actgcccgga
     7501 aacaaggtca caccataata attgttttcg gtatccagtt taatgttgct gtttatccac
     7561 gccattaggt tttcaggatt ttttttgaac gactttattt cctttttact gaaactgttt
     7621 tgaagttcgc tgcgccatga gctcagcagc tcgcgcccga tgcggggact cagtacatat
     7681 ttcgtataaa tatcttccgg acaattgttg cacgaaaatc tttcagcgtt catcaaatga
     7741 ttcaataaaa tatccgcccg ggtatcgcgc aaatcttttt cgctgatcac agaaagcagt
     7801 tccgcagcgg tttctttgtt gtcagcacga ttataaaacg atgcaatttc ggaataatta
     7861 ccacggcttt tcaaaataaa attcaccatc gcttcaccgt aacgatcatt tttatattgc
     7921 tcacgcaacg aaacggtatc ggggaaagtg acttcgtaag cggctctgat gacatcttcc
     7981 tctttcaaac ggcgatcgtt ttcttctttt tttgaagtgg ttggtgttac tgttttaccc
     8041 aacgaaggtg gtaaaatctg aaaagcaaac acagaactgt cctgttgaat ttcataagga
     8101 tcgaaaatga tttcgagcac cgattcctgt tcagatggaa acaatttcca tgtgaaatct
     8161 ttcccatcat tcacccaaat catcagatct ccgaaagctg tttcaatgct gcatcggcca
     8221 tcattgccgg ttttcagcga agcaaaagta aaaaactcag aatagttgta cacctgaaaa
     8281 tcaaccatta ttccctctat gggttttcct tgtgcatcca caaccttcac gacggcttgc
     8341 ttagatggtg cataattcga caataaattg agtttcgaat acaaaggttt ctgcaccaaa
     8401 gttttttcgg gaccattgta ttgtccgaat gtgtttgtat gcaccataat ggctctggta
     8461 gcgggccccg agaaccagcc catattgagt tccggatcgg gttcgcaggc gcccatgaaa
     8521 taccatttcc catcaaccca aacttcaacc catgcatgat tgtcgtcggt gtgcgcccag
     8581 cgcggcgtgt acacttgtcg tgcaggaatt ccgatgctgc gcatggctgc aaccgtaaaa
     8641 gtactttctt cgccgcagcg gccataagcg gttttgatgg ttgccagcgg cgatgatgta
     8701 cgctcatcgg ttgaacgata ggccactttt tcgtgacacc agtgattgac ttcgagtgca
     8761 gcatcgtaca tgttcatgcc ttgtacacgt tctttcaact caggcaggaa cacagcccgc
     8821 gcttcatctg gattttcatt attcacacga aaaggcaaaa caaaatgcat aaatacttct
     8881 gctgggatcg atttacccca tgaaaactcg cttctggttc gcaaagccag ttgcgccatg
     8941 gatttgaaat attcaggact taaattcgca atatcgctca aaggcgcata ggcaatcaga
     9001 aatttagtag cttcggccag ttctggtgtt tccgcttttt caacaaccgc aaacaaatcc
     9061 ggaagtcgcg ctccaaatgt agcttttcga tcactgaaca atttgttcat gttctcatac
     9121 gcaaccttat ccttcatcag cacctgcgaa aacaaaggcg cagcgccgat tgtaattccc
     9181 caaaccgcaa caaataaaag aagactcttc atatagttta tttactctac aataatttca
     9241 tccaaaaaca accatgtctt tcctcctgca cccgggtgcc agtcggggtt ttggcgcatt
     9301 gcactgcaat atacttttac aaacttcact ttctgtactt tgaaagaaac catcaaatcc
     9361 tgaacagaag tagctgcatc ggtctgtgga acattgtttt caacttcgcc cacttttttc
     9421 caatccgctg catcagccga tacaaaaact tccagtttca gcggatgata aatccatgcc
     9481 cccgtgcttt gcagcgagcc aacacttact ttgctgattt ttgtttttgc accgaggtta
     9541 acgatggctt cgaaatcaat tccttcgtag ccctgccaga ggttgtcgcg ataggagtat
     9601 gtaccacgca gtccgtcggt catgttggta tctccaccgg ccacatattt tttatggaac
     9661 ggataagtga tagtatattc tttcccaagc gctttatgcc agcaaacttt tctcgattcc
     9721 acagcagttt ttcccgaacc ttcgcgcata gccgcaacct taatctcagc gctggttttc
     9781 ataacaaccg gtacggtata ttttaccgaa ttgaaatcag gatcactgcc atcggtggta
     9841 taatacatgg tcagacccgg ctgcccgtgc tcaacaacag caacaaatgc attctgtgct
     9901 ttatcaaatg aggtggtcaa ttttatcgga tcgcgctcgt agccatattg tacattcatc
     9961 tgatcgagac gtttataatg gacggctaag cgatcatgaa attccttgaa atttcttcca
    10021 gtggggtcgg accacatgac ctcgcaaaga gcacacagac gcggaaacac acggctgtca
    10081 atcacttcct gcggtgccgt ttcggtccac atgcagcatt cgacacctat aatatgatga
    10141 cgtttgtcgg cgggcaaatc agcaggaacc ggttcaaaag aatacacatg ttcgagattt
    10201 gtgctggtgg tgctgtaatc gaaataacaa tgactggtag gcgacatgat agcatcgtgt
    10261 ccctgcatgg ccgcttccat gccgccttcc atgccccgcc acgactgcac cgttgccgac
    10321 ggggccagtc cgccttcaag aatttcgtcc cagccaatta actggcgtcc gtgtgcattc
    10381 agaaattttt caatccgctg attaaaatat ccctgaagtt cttcttccga ttttatgttt
    10441 tcggacttca tgcgcgcctg acatttcgga caggcttccc aacgcacttt cggagcttcg
    10501 tcgccaccaa tatgaatgta ttttgatgga aacaaatcca tcacttcagt aaggacatcc
    10561 tgcagaaaaa taaaaaccga atcattgcct gcgcaataaa tatcattaaa tacaccccag
    10621 cgcttttcaa cttcaaaagg tccgccggta caggataatt gcggatatgc agccagggcc
    10681 gcaacactat ggcccggcat ttcaatttcg gggatcactg taatatagcg ttcggccgca
    10741 taggcaacta cttcgcggat ctgatcctgc gtgtaaaaac cgccatagac actgccatcg
    10801 tcttctttgc gccaggcgcc cactttcgtc agctccggat atttttttat ttcaatccgc
    10861 cacccctgat cttcggtcag atgccaatga aaaacattca ttttatgata ggccagtaaa
    10921 tcgatgtagc gttttacaaa ttccacatcc ataaaatggc gacaacaatc cagattcatt
    10981 ccacgccacg aaaaccgggg tttatcagag attttcgcac atggcaattt tatcgatgca
    11041 atatcaatgt ttttctcata tccaaaacca acaggcatca tctgacgcaa agtctggaaa
    11101 gcataaaata ttcctgtctc cgtcattgca atgattttta tttcattgcc actgactacc
    11161 aaatgataac cttcggaacc gcaccactta gccgaatcat cacgtaaaac agaaatatac
    11221 ctgatgtttt tatgtcgcac cgaatcaaat tgcaaagcga caccagttga tgtaaacagc
    11281 tgatttacaa acaaatgcga tgttcgcaaa attccaggac ttgtttcggc aagcatgcga
    11341 acacttgcat caaaattaaa aactccggaa ccaacagaaa tactcaatgg cttgggaata
    11401 aggttaacat tctgattctg tgctgataaa acagttatca aagagaagaa tccaacgaag
    11461 aatccaaatt ttttcatact gggggggata tctgaattta taaacaaagt ttaaaaaaaa
    11521 atacaatatg acggccatct tttatttcat ttaccgcaat ttacaccaaa gggattgatt
    11581 ttggcaaccg gcaatttttt taaagtatta tttatggatt cttattttcg tatcatcatt
    11641 gataacatca tatatttcaa caatatctgc gtttttcaat gagatgcagc ccaacgtcca
    11701 gtttttatgc tgatctatcc attggtcctt cccttcaggc acaccatgaa tgcccacttc
    11761 gcccccaatg gtggcatctt gcggaatttc accttcatct tttgctcttt taaatttctt
    11821 ccatgagtct ttattggggt aatcgagcca gataaaacga gtccaattcc ggtgcggata
    11881 tttgtctctt ataccaaaaa ctccctccgg ggtacaacca tcgccttcca tacgcttgtc
    11941 attcaccgga tcgggcccca gcactaccgg atagcttttg aggatttcct gattatattt
    12001 tacatagaga gtgtatttgc ttttagaaat aacgatttga atatctttct tgtcaatatt
    12061 cttttcatca agaacctgac taagcggaag agtataatta atatacctgg tctcttcttt
    12121 tttctcatca cctgaacagg aaataagcaa aacagcaaca ataataaaaa gtactttcat
    12181 ctgtttcaat ctttctgcta aaataacgat taatcataca caataatatt gcataccttt
    12241 gaattaatat aaaaatgcaa acttacaaac acaatcgttt accattttta gaaaccatcc
    12301 gggtcgttga tggtataatt gaatattctg actatcatat agaaagaatg cgtcagacct
    12361 gcttatttca ttaccatgaa tttggtttgg agaaaatact tgacaatttg agacttcctg
    12421 aaaatggaat attaaaactc aatatttggt acaaccaaca cgaaaaagaa atcaaaattt
    12481 ccgaatatgt accgacttca ataaaaaaaa tcgaactgat agaatgtgat cccgatttta
    12541 attattcatt caaatattcc gaccgcagct atctgaacaa tcttcttaaa tccgcttccg
    12601 gagctgatga gataatcatt gtcaaaaatg gaatgatcac cgatacatcc aaagccaata
    12661 ttgttttcga gaaaaacgga aaattctata cacccgatac ttttttgctg aatgggacca
    12721 tgaggcaata tttactcgga tcgggaaaaa ttacagaagc ctccatctat gctgaaaata
    12781 ttttcgacta cgaaaaaatg tactttatca atgcattgaa tccgttgggg aaaacaccgg
    12841 tttttaatgg ccgcatttta tagcgtttca atgtaaaatt caaatgttta cgaaatgtaa
    12901 atgtgtgaaa tattcaacta aattgaatat ttttgttgta gcatttttct aaattcccac
    12961 catgaaaaaa ctatttacac tcattgcatt cgtgttttcg tgcacgatat tgtcagcgca
    13021 aagtatcact gttacttcac caaatggcgg cgaagtgttg cctggctgta ctattaaaac
    13081 cattacctgg acttcgtcgg gaactacgaa ttattattcg atcgattatt ccaccgacaa
    13141 cggaaacacg tggacatcga ttaccagttt ctacaataca gtgtcaggga cttacaactg
    13201 gacagtaccg aacacgtcat cgggaatatg cctcatcaga attgttgact caaacaactc
    13261 atcgattttc gatatcagcg atgctgtttt tacagtcaca tccccattga cggtaaatgc
    13321 accaaacggt ggtgaatcgt ggcaggcagg aacctcgaag agcattcagt tcaatgccag
    13381 cggcacctca aattattacg atatttatta ttcgaccaac gccggggcca actggacatc
    13441 cgttgcaacc aacacctaca atgtgtccgg tacatacaac tggaatgttc ccaacacgcc
    13501 ttcgaccaac tgtctggtga aaatcagcga tcactcaaac acctgtatga ccgacatcag
    13561 caatggattg tttaccatta cagctgctgc atcatctgtt acagtaacat cgccaaacgg
    13621 tggcgaaacc tggtatgtgg gtcagtcaaa aaatattacc tggacagctt cgaacaccac
    13681 caattattat aacatcgatt attcgaccga caatggaacc acctggacat cgcttgcaag
    13741 ctcatattac gcggtgaccg gaacttttcc gtggaccgtt ccaaacaccc cgtcaaccaa
    13801 ttgcattgtg cgtgtgaccg atgcaaacaa tctgccggtg accgatcaga gcaacgctgt
    13861 atttaccatt gcagctccat tcatcacagt tgtttcgcct aacggcggcg aaagttacga
    13921 atcgtgttac ggacaataca tcaattttca gtgtggcggc acgtcgagtt attacaaaat
    13981 agaatattca accaacaacg gaaccagctg gacaaccatc aattcatcgt attatgcaac
    14041 aggatccaat gtttcatttt tatggagcac cattccacaa aacagctctg ccaactgcct
    14101 tgttagggtt actgatgcca acaaagccac ggtgctcgat caaagcgatg ctacatttac
    14161 cattgttcct aatgtagaaa tcattgtcac cagccccaat ggaggcgaat cgtgggaagt
    14221 aggaacatcc agaataatta catgggtggc tgcaccaact tcaacacggt ttaatgttta
    14281 ttattcggta aacagcggtg caagctggac atacattaat aatacgtatt caacaagtct
    14341 cagctggact gttcccgaca atgaatctac aaccgcttta attaaagttg ttgactacaa
    14401 caacagttgt atcaatgatg taagtaatac ggttttcacc attgcgccgc cgacgccggt
    14461 catcactgta accagcccca acaccaatgt tacgtggtat cagggaaaca gcaataccat
    14521 ttcatggaca tcgcaatatg ttgaatcgcc ttttgtcacc atcgaatatt caagcgacaa
    14581 cggaagcagc tggaacacca tcgctgctgt gacagaaaac gacggatcgt attcatggtt
    14641 gattccgaca gttgtcagca gtcaatgcct cgtacgggtt tcagagtatt tgaatcctgt
    14701 ggtgtttgat gtcagcaaca catcattcaa tatagcagca ccctttatta ctgttacatc
    14761 gcccaatggc ggaaacattt tcggtggatg cgatgcgcac accatcacct acaccaaagg
    14821 cggaatcagc agcaatgtga aaatagagta ctcgcctgat aacggtgcaa cctgggtgaa
    14881 tatcgtttcg agcacttctg catcaggatc ctataactgg agtattgtgg ctaatattgc
    14941 ttcaacacag tgtctcatcc gggtatcgga tgctgctaat gccacggtaa atgatgtcag
    15001 caatgccgtt ttcacgattg agaaaaacga tgatattttt gtcacctcgc ccaacggtgg
    15061 agaatctttc gaagtcggag cacaatatac agtcagttgg gcagctgctt cagggcactc
    15121 tcagttttca gtatattatt cctacgataa cggaacaagc tggagtacac tgtattcaag
    15181 catctattca acttcgtttt cgtggaccgt tcccaatacc ccatcggcaa actgcctgat
    15241 aaaagtggtc gattataaca acagctgcat ttatgatgtt tcgaatgcca atttcagcat
    15301 cgaaccgccg caggcaataa taactgtcac cagtccaaat accgcacaaa cactgtatgt
    15361 agcaaattcg cataccatca cctggaccca tcagtacgac gatgccgatt ttgtgggaat
    15421 tgattattcg tatgataatg gtgccacctg gataaacgta gtttctgtaa ccgaaaatga
    15481 cggatcatat acatgggctt cgattcccaa tactccatca acacaatgtc tggtgcgggt
    15541 ttacgaatat aacaacatga gtctgattga tcagagcaat gtcaatttca ccattgcacc
    15601 gccctttatt gtgctgaatc agcccaatgg tggtgaagtg atgaccggtt gcggaaccta
    15661 taacgttaca tggacaaaag gcggcaccag cagttattat ttcctcgatt attcactgaa
    15721 caacggaacc acctggaata ccatcaactc aagttattat tcaacgggta ccaataattc
    15781 attttcatgg aatcccgttg tgaatcaaag ctccacgact tgtctgttcc gtgtcaggga
    15841 tgcaaacaat ctgtccaccg gcgatatcag tcaggccgta tttacgatta caccaaacac
    15901 caatatcata gttacagcgc cgaatggtgg cgaaacattg gaaattggcg acacctatca
    15961 gatcacctgg gtgtcggcgg gaacttcaac acaattccgt gtatattatt ccatcaacaa
    16021 cggagcatcg tggacgacac tgacatcgag cacttacagt aattattaca actggaccat
    16081 tcccaatgct ccatcaacac aatgtctagt gcgggtcgaa gactataata attcctgtat
    16141 caatgatgtc agcaatgctg ttttcaccat tgcagaaccc gcgcccaaca ttacggtgac
    16201 atatccaaat accgcagtga cactgtatta caatgctaca gcaaccattt cctggacgag
    16261 cgaatttacc gtttcttctt ttgtaaatat tgaatacagt atagacaatg gcgcatcgtg
    16321 gaccaccatt gcatcgccca ccgaagatga tggctcttac aactggactg ttccattaac
    16381 ggcttcaaca cagtgtttgg tcagggtcag tgaatatgga aatcctgccg tgtttgatgt
    16441 aagtaatgtg aactttacca ttgcgccacc ctttgtaaca gtgacctacc ccaacggtgg
    16501 tcaaaccatc gaaggctgtg ccagcgccaa tatcacgtgg tcgaaaggcg gcaccagcag
    16561 ttattataaa attgaatact cgattgacaa tggcagcaac tggacggtga taaattcttc
    16621 ctattataca accggaggaa gtttcacatg gagtccggtg gccgattata attcaacaca
    16681 agcattggtg cgggtttccg acgccaatta tccactggca accgatcaga gcaatgccgt
    16741 atttacattg cttaaaaaca ccgacattgt tatcaccaat cccaatgggg cagaacaact
    16801 cgaagttggc accacctacc agataaactg ggttgccgac gcatcggtat cgcggttcaa
    16861 tctgtactac tctgccgacg ggggtgcgag ctggacatac attggcaata cctattcaca
    16921 ttattattca tggaccattc ccaataatat ttctgcgcat tgtttattaa aagttgttga
    16981 tttcgacaat acatgcagaa acgatatcag tgatgccgaa ttcagcatta ctccaccaac
    17041 accagtcatc atcgtgactg ctccaaatac agccaccaca ctgtatttca caaagactgc
    17101 tactatttca tggacatcgc aataccttgt ttcgcctttt gtttccattg agtacaccta
    17161 cgataacgga ctcacctggc acagcattgc caatgccacc gaaaacgacg gaagctactc
    17221 gtggacaata ccagagactc catcgacaca atgtaaagtc agaatttcgg aatatcagaa
    17281 tccggttgtt tttgatatga gtgatgtgaa tttcagcatc gcctatcctt atattattgt
    17341 tacagcgccc aatggcggcg aagtgtacga aggctgcgaa acggcaacca ttaactggac
    17401 ccgtgcaggt gtcagcaata attataaaat tgaatactct tcgaatggcg gtgtaaactg
    17461 gacaatcatc aacagttcat ataatacatc cgggtcaagc tattcgtgga caccgctccc
    17521 cgacctgact tcgaccaatt gcatggttcg tatcagcgac gctaattatc cggcagcaac
    17581 ggatatgagc aacgccgctt ttaccatcag caaaaacgaa gatatcattg ttacatcgcc
    17641 caatggcggc gagttctggc aggttggaac caccaaaacc atcacatggg ttgcagcacc
    17701 caccagcacc caattccgcg tgtattattc ggttgatggc ggtacaagtt acacacagat
    17761 caacacaacg tacagcacat cgctgtcgtg gaccattccg aatattcctt catcgtcgtg
    17821 taaaataaaa gttgttgatt acaacaattc gtgcatttat gatgaaagca atgattattt
    17881 tgccattgtt cctgccgatg ttgcactcac ttatccaaac ggcggtcaga gcctgtatta
    17941 cggttcgagc tattcaatca catggaccaa tcaatacatc aattccaatt ttgtgaagct
    18001 tgaatactca accaacaacg gcgcttcgtg gaatgtgatt gccagtattg ccaacaacac
    18061 cgggagttat tcatggtcag taccgcagga tttcagcacc caatgcctgg ttcgcgtaag
    18121 tcaatacaac gacccgaatg tttttgatat cagcgacaat gtgttttcaa tttcaccttc
    18181 gattgttttg aatacaccga acggagacgg tggcgctgaa gtatggcgcg tttgcaccga
    18241 aacaagtata gtgtggtcgg ccaatggcga tggaaattat ttcgtcattg agtattccat
    18301 caacaacgga ttgtcctgga acaccattga atcgaattat tacaatacat cttcaaccat
    18361 cagttacaat tggacattgc cgaatctgcc ttccacacaa tgtctggtaa gggttaccga
    18421 taaaaacacg ccggtaaaaa ccgatgtaag cgatgcaaca ttcaccattt caccagccat
    18481 taatcttgta aatccgaatg gcggtgagag cataaccggc ggtacgccat ataccattaa
    18541 ctggacatcg gccggagtat cgaactatta caatattgat tattcttccg atggtggaac
    18601 cacttgggtg aacatctgtt acaatcagaa tatcaccacc aattcttatg tctggaatgt
    18661 tccgtctttg atttcatcca attgtaaact gcgcgttacc gataatgtaa atacctgcaa
    18721 aaccgacatg agcaatcagg ttttcgccat cggaacagct gcagtgaata ttactgtaac
    18781 tacaccttcg ggtggacaaa cctgggcggg atgtaccact gaaaccattg cctggaccag
    18841 caccggaaca tcaaatgtgt acgatatttt ctattctacc aacggtggcg catcatggat
    18901 tctgattgcc gataattatt ccacactggc aaaaacatac gactggacac ttccgaatct
    18961 ggcttcggca aactgcagaa taaagattac agatgccggt aacagcagct attatggcac
    19021 cagtgcagca aatttcacca taacgacagt ggtagccaat gccggtaccg atgcagctat
    19081 ttgcgcaggc aacagtctga atctgaatgc cagcggagga gtttcctatg catggcttcc
    19141 caccgcagga ttaagcaatg caggtattgc caacccggtt gcaacgccat cttcaacaac
    19201 aacatacacc gtgtatgtta ctgatataaa tggctgcaca caaaccgatc agattaccat
    19261 taatgtgaat ccgattcctg cagcaccagt cgcatcgagc aattctccgg ttaatctgaa
    19321 cggaagcatt gagcttacgg caagtacggt gaatatggct ttctacagct ggacaggccc
    19381 cgatggattc acatccacca gtcagaatcc cgttattccg aatgcaacag ctaccaacag
    19441 cggaacctac tcggtaattg ctacagtagc cggttgtgcc agtacagccg ccacaaccaa
    19501 tgtggtggta accggagttc cggccactgt tgaaattgca ggaaatgttc tgactgaaac
    19561 aggttcaccg gtctcaggtg taacattgac actcagcggt gacggtaccg acacctatgt
    19621 cacttcagcc aacggatatt atgattttac actcaacaac agtggtacat atgtggtcag
    19681 tcctgtaaaa gacaacgaca ttgttaccaa caatggcatt tcgaccatgg acattgttct
    19741 gatgcagcga cacattttaa atgtacagtc gttaccgaca gcgtacagtc gtattgccgc
    19801 cgacgtaaac cgatcctcgg gaattaccac gctcgatatt gttctgacac gctcggtcat
    19861 tctgcaaacc gccactgagt ttccgaacgg agatatgtgg acatttgtaa acagcgacta
    19921 tgtatttccc gatgttcagg cgccatggtc gtacgaaatg tcgagatcat acagcgcagc
    19981 tgctgaagcc gaagatcagg atttcatcgg tataaagctg ggcgatgtca acaattcatg
    20041 gaacaccaat attgccaaat cgaccgaaac agaactgata ctcaaatccg gcgcaacgca
    20101 ggcccagtca ggtcaaacat tatcaatacc atttaccgcg cagaactttg atcagctcag
    20161 cggattgcaa atgactatag catgggatac cactgtattt gactttaatg cagcaacagc
    20221 caatattccg ggtttgtatt tcggcgaaaa ttatactgcc ggcggaaaac tgactgccat
    20281 ctggagcacc ggcgatatgg ccggagtgag ctatgcgccc aacgatattc tctttaccat
    20341 cgatttgatt ccggtaacag atcaacatgt gattaccgac atttcggtgt gcagcgacat
    20401 taccagtgcc gaagcctacg atgcagcgct gattccgatg aatatagttt tacagaacag
    20461 cagcattgaa ttgaatccgg tcattacaca gattgaagat tccggttccg gtatacaact
    20521 caattgttat ccgaatccct ttgatgatcg cattattatt gaattctaca attcaaccga
    20581 ttccaaaaac accctgcaga ttttcaacaa tctgggcgaa attgtgtatg cgtcaacaac
    20641 ggacatgcag caagatatca accggattga atgggatgga tcggatatga acggacataa
    20701 actgcctgcc ggaaattact ttctgaagct gatttccgac gacgcaacaa gttctcgaaa
    20761 actcgtgata gtgcggtaat agcagttgaa ttctttagtt ttttcactat gaaatatttc
    20821 aaaataatat ttgcacttct ttttctgcca ttgatttcac aggcacaggc tgtttatttt
    20881 cggctggggt caggaagcaa tattcagggg actgaggtcg taattccagt cacagtgcag
    20941 aatttcagca atctgatttc aatgcagggc acggtatcct tcaatcctgc agtaataagt
    21001 tttgtaaata ccgaaaacta cggtgtgagc gatttgtctc cggccaattt cggaacaacc
    21061 aatgccgcaa cgggccacat ttctttttcg tggaacgatc cggatttatc cggagttagc
    21121 ttaagcgatg gcgataccat tttctgcctg cgctttttga tgattggatc tgcgggcatg
    21181 tcttcggtga ttgaaataaa tgatgatatc acaccaattg aagtgattga taaatttttt
    21241 gcggcaaaaa ctgtgtacac acaatccggc gctgccactg tgcaagctgg acaagccgtc
    21301 agcggattgt tgctttcggt tgacgaaacc gaagggctca gcggacaatc ggtgcaggtc
    21361 cctgtcaccg cgcaggattt caccgatctg ttgtcggcac aaggcagcat tgcttttgat
    21421 ccggccatca tcagctttac cggaatcagc ggatacggat tgcccggcat gaatgcgtcg
    21481 aatttcggta tcagctcagc aggatctggc atcatcactt ttagctggaa tgatgcaaca
    21541 ctggcgggag tcagtctcat cgacggtgcg gaaatatttg ttctgaactt taatatcatt
    21601 ggttctgccg gacaaattag tcccgtgcaa ctgacaaaca atcctacgca actcgaattt
    21661 gccgatataa cttattcagt tgtcacctac tcgctttcgc aggggtcggt tacagttgga
    21721 acgccattca ttcctgaaga tattttcata tttgcggaca gtattgaagg agccatgggt
    21781 caatatgtgc aggttccggt gcgggtttca gatttttcaa acattatttc cgcgcaggga
    21841 agcctgagtt ttaatccggc catcgcacaa ttcgatcaga ttgtttcgtt cggacttgcg
    21901 gatatgacaa atgcaaattt cggaactacg caggcagcat caggcctcat cagtttttcg
    21961 tggaacgacc ctgacctgag cggtgaaagt ctggcagata gtgccatcat ttttacactt
    22021 cagtttcatt tggtcggcac tatcggacaa cagaccccgc ttagctttgt gaactcaccc
    22081 actccttttg aatttgtgca aaatgatttc acggtactga attactcttc agcgcccgga
    22141 aatattaaaa ttattgatct gtatgagctt gaagttgcat cggtaagcag cacagagatt
    22201 tgtgcaggcg ctgatctgac gattcagtat tctgccattg gaaattattc tgcggccaat
    22261 gtgttcacag cttttctatc cgatgcttcc ggatctttcg ccttgccggt tagtataggc
    22321 tcactgacat cgaatgtttc aggaagtatt aatgtcacta ttcccgcaaa tacagcgacc
    22381 ggaatacatt acagaatacg ggtcgacgca tcggatcctt attatgttgg tgatgcaaac
    22441 gcaactgata taacaattca cgcgatcccc aatgccaacg cgggttccga tcaggacatc
    22501 tgtattggaa attcagtgac gctcactgca accggcggcg tcagctatgc ctggaacagt
    22561 ggtcaaaaca caggcgccat caccgtttca cctgcattga atactgaata tattgttact
    22621 gttaccgacg cttttgggtg cgaaaacagc gacacagtga atgttgttgt tcatgcactt
    22681 ccctctgcca atgcgggctc cgatgtaaat atctgtttcg gcgagtcggc atcgttgagc
    22741 gccagcggtg gaaatgcata tgcatggaat tctggtcaga atacagcgga tattacagtg
    22801 tcgccaacgg caacaacgca gtttattgtt tctgtaaccg acatctggag ctgtcaggca
    22861 gctgatacgg ttgtggtaaa tgtttttcag gtctttgcca atgcaggctc cgatgaagat
    22921 atctgcagcg gcggatcaac tttgctgacc gccaccggcg gaacttcgta tgcatggagc
    22981 accggccaaa acacagatgc aatcacagtg tcgccagttg taagcagcaa ttatatcgtt
    23041 actgtgaccg gtgtaaataa ttgtacagat ttagatactg tttctgtgat tgttcacgcg
    23101 atccccaatg ccaacgcggg ttccgatcag gacatctgta ttggaaa
//
LOCUS       DAOPKE010000043        22611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2420_length_22611_cov_12.417140, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000043 DAOPKE010000000
VERSION     DAOPKE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22611
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_22611_cov_12.417140"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..>3275)
                     /locus_tag="PKY63_07050"
     CDS             complement(290..>3275)
                     /locus_tag="PKY63_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPF00406.1"
                     /translation="YNNINFALIYTSVNQTDAEKYKLVFAIVNSATYNWRLEPFAVQI
                     MKNGMFAYNHIRVAASTINHYTTDYNSAEYMVLTILDECKNEAVARIVGHNRFRTIDF
                     EKEVKRDNNLMRRVIKHLRNKTDMAISILADNQIPIYFKGDQQDIISEKTIIPQSEKL
                     VVKFRFAYIDGTIQYSIQTELAGKKVKLTDKNTLILNYDPCWLIMDNKFIQVADNVDG
                     KKIQPFLTKDQVIVEAVQVEPYLKKFVRQIIATYPFELTGIPFSHFGENPVPVLRIET
                     SLQNDIMLMLRFRYDNVEFEYGSDIQSHVALQKKENNFSFDIIRRNNEFETTVVEWLQ
                     DNGLVPAYASYFLPLKVQIDDQNKSASEKMYDIINYINEYSATLQEKGFDIAQLLDKK
                     FFTGRIELMQQFTEDNDWFDLKIFVQFDEFKIPFSALRKNILENNRELVLPDGSIAIL
                     PEEWFARFRDLAMISKPEGENVRVKKSQYSFISNILEGNNRVEDFIETAGKGDFQIED
                     APKGLKKELRPYQLRGMSWFMFLYKNNFGGCLSDDMGLGKTIQVLAFLLKMREISKEF
                     RQKTQLDLFSNETTIPHAKTSLIIMPLSLVHNWENEIRTTAPELKFLNYTGLGRRFNQ
                     KQVEKYDLVLTTYGTVRNDHEKLSKFPFRFVFLDESQYIKNPESKIFAAVSKLNAEQR
                     FVLTGTPVENSLIDLWSQLQFVNPGLLGDLKFFRDRFYQPIEKRGSKSSEKKFRQIIN
                     PFILRRTKNEVAKELPELTEKIHFCPMTPEQLEIYEKRKSEVRNYIIESTKKYGTDKA
                     YMIILSGLMRMRLLANHPGITEKDYPGGSGKFDQVTESIRKVLSGGHKLLIFSQFVKH
                     LVLYASWLEDEGIPFLMLTGQTNTIQRQNMIRQFQKGYDYPIFLISLKAGGVGLNLTA
                     ADFVFLLDPWWNPAVEQQAINRTHRIGQNKKVISYKFITEGSIEEKIMALQEKKMDLF
                     NNFINRKISSNISSEDLVGLLE"
     assembly_gap    3276..3375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3376..4827
                     /locus_tag="PKY63_07055"
     CDS             <3376..4827
                     /locus_tag="PKY63_07055"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPF00407.1"
                     /translation="DADQRILGLKIYSSDNAAALTQVSVKMTGTTDINDVSAVKLYFT
                     ADSNKFFTGNGSSLLDSENSSTGAIFLSCNQHLMADTNYFWIVYDVSAAASEGHQLDA
                     VVDSVVVDGDAFSVADQSGARTILLTNTLLFSCNDAGSKHYRIPAIITAPDGSIVTAT
                     DKRWNNSADLPNDIDIIIRRSTDKGETWSAPLTIAGGDTTLGFGDAALCVDKTNGNII
                     CLMVSGPGLWGATAANPLRIMKCVSTDNGLTWSPPVDITDMIYGAGCSNILTKTWWSA
                     FVASGSFVQFSNGRLAAVITVRRTSAYSIDNYMIYSDDHGVTWNISTNQAEADGDEAK
                     LIELADSTVLMSIRNTGYRRFNTSADFGLTWGNAYSEDEITDPNCNGDILRYTCVNDG
                     YLKNRILHSIPFALNRSNVSVLLSYNEGNSWPVRKTIYSGPSAYSSLCVLPDGTIGIY
                     YEVGESEIYQMYFSRFSLDWLTDGADHFSSAIK"
     gene            4967..5407
                     /locus_tag="PKY63_07060"
     CDS             4967..5407
                     /locus_tag="PKY63_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPF00408.1"
                     /translation="MNKIIVLYDSGFGNTKQIAQAIVEGMGDSASLLHVSEFTREHLN
                     NLSFIVAGSPINGFMPTEKMQAALKTIPAGALKGIKAASFDTRMHVWIHGDAAKKISK
                     RLAAAGAEIIAVPAFFHVTGKEGPLAEGETERAIAWGRKLVESF"
     gene            complement(5442..6689)
                     /locus_tag="PKY63_07065"
     CDS             complement(5442..6689)
                     /locus_tag="PKY63_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00409.1"
                     /translation="MKETELIINFIEDKAYLCEGSPLSFEQLSFPPVAKNFKAPAHWR
                     VKVLNYRESENRIFVAVLSYKTGLAEYPPQQLRIKEQLSTISQITFKSIDTEGLLATI
                     RGNEPTKGFINEPISELYSEEMQQEILLDIQAPAQKRMLILDETFSVPINELCFRFGG
                     VAFNHTFRDYHKPIELTIENYELREEFDAVKNYFSNALKTKNIHVVAHVEIENDTITC
                     MRVQSNEIASIDKKMIDNVRFEFVRDTTKKKLNAEVDKTLFTMDEYFESLTESRLQSN
                     AFYENENELFEDLISITQTKHYKHLRFLSSQHAFHIMKLRFVLKPFSFLFLLEGERHY
                     HIIWETIDTKEATYIWHTDKDLSMLKMTLRKIEDIINVIKVQGKIAYLNANEDSFQRI
                     RHDYSELIDGFVKWKGELENYLT"
     gene            6787..7632
                     /locus_tag="PKY63_07070"
     CDS             6787..7632
                     /locus_tag="PKY63_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00410.1"
                     /translation="MSTRILLIDDDENYVAKLSADALKAGFEIVSDDNLESGIIRLRS
                     DRRIAAVILDGRCPLSASGNQPAKLSFVTHALQQIRELEDEYNRAIPFCINTNTPEEF
                     LEEYEGIARVFKKDSEHPELFAHLRDLIKALPETRVREEFGGVLTKFSNRFDDETTEL
                     LMDVLLALESADKATIIKHLGVLRLLLESLIDEVCQEKLHRPVEAYMHKEGSRTRRIL
                     DDLRRRVIPIELYDSAIQIYRICSRYGNHADPVAPGAIVLSPTRYTYQRLVYGLLELL
                     EYLLA"
     assembly_gap    7716..7815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8000..8087)
                     /locus_tag="PKY63_07075"
     tRNA            complement(8000..8087)
                     /locus_tag="PKY63_07075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8051..8053),aa:Ser,seq:tga)
     gene            8267..9730
                     /locus_tag="PKY63_07080"
     CDS             8267..9730
                     /locus_tag="PKY63_07080"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPF00411.1"
                     /translation="MKKLLLLLVIGFCTINIYAAPKKLNVYISYCTFYSPQDGPYVET
                     YLAYNGNSVNYQKNENGLLQAKLEVLILFKQNDSIKAFRKYNLHSPEMKDTTGFNLLF
                     HDEQRISVPNGDYSMEISIKDAYGTEPAIVIAEPVTIEYTNKEIQISGIELLQSAEKT
                     VTPGATSKAGFDLLPLPLNIYPTSVTKILFYTEMYNMLSILGDSSKYLFRTYIEEMQT
                     SRKIPGLLFQKRMDSKNTEAVIHEFDITSLPTGSYFLVIEIKDRENMLMAANQVYFER
                     WNDREQDMMIVDESYVAASFVSRITQIDSLKEYIRSIRPISTQMEKVFVDNNIKTADL
                     ADLQRFFLNFWMQRDEMAPEAAWIEYYKQVIKVNANFGTRIRKGYETDRGRVYLMYGE
                     PNSITSVPHEPAAYPYEIWHYYKVLNQSNRKFVFCNADLVTNDYELIHSDAIGEIYDA
                     NWKSKLLKRNFTTNSLDEQTPDFGWGSKVDDYFSTPH"
     gene            complement(9887..10681)
                     /locus_tag="PKY63_07085"
     CDS             complement(9887..10681)
                     /locus_tag="PKY63_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPF00412.1"
                     /translation="MKTLSNILFATLWFVSVASIAQSQTPKEKFSRANALYNEKKYEE
                     SIVQYNELLRSGYIKDEVYYNLGNALYRNNNAAAAIWCYEKCLLLNASHEDAAANLAF
                     VNKTALGNQEIVPENFFIMAWNWLSALMSWQWWTNFFIIVLVVFLVALILFLTSASNI
                     NRKIFFLTSVILFVVLLLALAQSISGNYNIHHSKNAIVVDEAVQLRSSPDKDGTGIAM
                     IQPGIKVHILEIKTPWLRITTPDGTQGWVPEERVVRLNAVSPLLPE"
     gene            complement(10678..12543)
                     /locus_tag="PKY63_07090"
     CDS             complement(10678..12543)
                     /locus_tag="PKY63_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPF00413.1"
                     /translation="MKTIFTLVCIIFSLTISAQIKFSASSDYSTVPVNSQVKVTYSLS
                     GGNAETFTKPSFEGFQVVGQSSYSGGGGMQIIVNGQLVQSSPGESKWIFTLVPKSTGT
                     YTIGPARVKVKGQTYSSNSISITVTNSGNAVKSQGQANNNTVQPDKNQPKQSTSGGKE
                     VFLTAVPSKSRVYEGEQITVSYRLYTRYDISQYGIEKTPSMDGFWTEELTDTKTAAKT
                     WEETIEGKRYIVGEIRKVALIAQKTGTLSIPSLELEAIVQIPNQQNYNPFSIFDQFFK
                     DPFGSSPIDPFAGFGVKTEKRKIRSNPFSITVIALPEKGKPESFNGAVGNFEVETEVD
                     RNKCFTGDAVVLRMKVNGNGNLPLIEYTEPGIPESFEWFDPDIIDDFYKTSDGISGTR
                     TFEFLLQPTVPGDFTVTPSAFSFFNPSTGQYQSVELPEYKIKVLKGNGSNVSSEKILN
                     EDIRHIHENIPFLMLSSKTFSFSVLYWIVVAVLILVAALILWYFRKRIQLKANVSEYR
                     MRMAMRQAKRRLKTAKSLLDQGKEEEYYTELSKALWLYLTDKFTIPFSELSLSNAREI
                     LLRSNVPAEISEEFLKILDECEFTRFAPAAGRMSQKELYEKSADLIVKVQTHAAK"
     gene            complement(12547..13284)
                     /locus_tag="PKY63_07095"
     CDS             complement(12547..13284)
                     /locus_tag="PKY63_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00414.1"
                     /translation="MMKILLAFTLAIITSTVVCAQNSLHNVIEGNKAYRKKEFDKAAG
                     LYRKSLEQKTEYDFQARYNLANSLYRNGDFEGARQTYSSLLNDSLDNRTQSRLYHNIG
                     NTYMQEENYEQSVIAYKNALRLNPSDEDARYNLSYALQMLKKQQQNQQNQQNQQDNQD
                     QQNKQDQQDQQNQQNQQNQDKNKDQNKDQNKNQQQKQEQEKNEISSGQADQMLKALQN
                     KEKQTQREIHAQPVKEGQGATNGYKDW"
     gene            complement(13281..14327)
                     /locus_tag="PKY63_07100"
     CDS             complement(13281..14327)
                     /locus_tag="PKY63_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF00415.1"
                     /translation="MYRFEHPQYLWWLSTLLLATGLFMLYYFARKKQLGQMFSENNLR
                     LSIPGFSKNKPLTKFILVASAAAFMIVTLANPLAGKKVDKQIQSKGSDIQFVVDISNS
                     MRCEDIKPNRLSKVKQIMLGMIDRMGSDRAGLIVFAGDAFIQLPMTIDQTAVSMFVRN
                     LDPELISRQGTHIEKALTTALISIDSSKAKYPAIVLLSDGENLEGNPEPIIGELQKRR
                     IPVHTIAIGTEKGGPIPVYNNSVRTGYKKDEGGNTVISKPDEALLKGLATQTGGLFID
                     TKNLGNAAESIMDELRKAEKTENRSMIFSDYKSLYLWLLIPAFLLLLIDFVLMERRMK
                     WQDVFKNFIERRTV"
     gene            complement(14333..15334)
                     /locus_tag="PKY63_07105"
     CDS             complement(14333..15334)
                     /locus_tag="PKY63_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF00416.1"
                     /translation="MEWINDLSFEHPWAFLLMSIIPLMIWWYIRNFRQSRHAIHASSL
                     KAIENAPKTFRERMFHLLPAIRFAAIAFIIVAIAGPQSYSFHDDYNIEGIDIMLVNDV
                     SGSMLAEDLKPNRLDAAKKVAQDFIAGRPDDRIGLVAFSGAAFTQCPLTADHAVLRQL
                     LMQIQNGIVRDGTAIGDAVGIAVDRLRYSSAISKVIILLTDGINNSGFVDPLNSASMA
                     KLYGIRLYTVGVGTTGKAPYPVKTIMGTSYEYVDVRLDEPMLQEMAAMTGGKYFRAID
                     NKSLESIYAEIDEMEKTRIKVARMSEKHDLFFIPVLIALVLLAFELLLRYTVLRIIP"
     gene            complement(15318..16256)
                     /locus_tag="PKY63_07110"
     CDS             complement(15318..16256)
                     /locus_tag="PKY63_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00417.1"
                     /translation="MRKPAALLIFILITSFSHAQNDIKVSLSRDSISVGELSVFQWQL
                     SLPTGTKVLQMPMLPDSMPSGIEVVKKKDIITEKKGSNDIYKQQLSVSAYDSGYFAIP
                     ALSALFLKDNDTVEILSDSLMLFCTTVPVDTTKAVKDIKDIYEVEGKTSFNWLWYLLA
                     GVLIAAAGVYFYFRLRKKKPMIPVEEIVWVNPTEAALTALRKIQESRSWFSVSPKDFY
                     TELVEILRKYLIYSRGIQAEEMLSSELIEAVTKAELPAESIHTLRGILNVADMAKFAR
                     FRPEPGQYEKSVSDAIKFVIDTRPVETNTDNNGMDK"
     gene            complement(16253..17131)
                     /locus_tag="PKY63_07115"
     CDS             complement(16253..17131)
                     /locus_tag="PKY63_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF00418.1"
                     /translation="MENDNFQLSKKVRRIEIKTRRLTDEVLAGSYHSAFKGRGMTFSE
                     VREYGYGDDMRSIDWNVTARFNHPFIKVFEEERELTVILLIDVSGSNNIGSTAVSKNE
                     RIAELAGTLAFSALRNNDKVGVIFFSDKIEKFIPPKKGQQHILRIIRELITFKPEGQG
                     TNITAALEYFNRVVNRRGIVFLISDFIDNGYERAFAITARKHDLVALRVIDKIESSVP
                     GSGLVLMQDSETGQLRVIDSNGATARMYNSWWNQQQQYLTQTAAKFNIDVVDFKTHED
                     FVKPLAKLFKKRGKRI"
     gene            complement(17131..18126)
                     /locus_tag="PKY63_07120"
     CDS             complement(17131..18126)
                     /locus_tag="PKY63_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF00419.1"
                     /translation="MTPTVDIHELNERIQRESAFIDLIRMELGKSIVGQKNMVDRLLI
                     ALFANGHLLLEGVPGLAKTLAIKSLASTIDAKFSRIQFTPDLLPADLIGTMIYSPKSE
                     QFNVKKGPVFSHFILADEINRSPAKVQSALLEAMQERQVTIGENTYKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMMKVLVSYPEKEEEKLIIRQNLAPQYPVPNKVASVEQIL
                     HARKLVHDIYLDEKIENYITDIVFASRFPARYGLVKYKDMINYGASPRAGINMALASK
                     AYALLNHRGYVLPEDIRAVAPDVMRHRIGLSYEAEAENITTDQIIIDILNAVQVP"
     gene            complement(18443..18910)
                     /gene="smpB"
                     /locus_tag="PKY63_07125"
     CDS             complement(18443..18910)
                     /gene="smpB"
                     /locus_tag="PKY63_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPF00420.1"
                     /translation="MKMAEKNTIQIKNKKAYFEYNILEKYTAGIVLQGTEIKSIRAGK
                     ASIAEAYCIINENEVYIRNMNISEYAYGTYNNHEPKRDRKLLLNRKEIKKLHNKIRET
                     GLTIVPLLLFISPEGYAKIEIALAKGKKMYDKRETLKEKDNRREMERKDTYKE"
     gene            complement(19080..19730)
                     /locus_tag="PKY63_07130"
     CDS             complement(19080..19730)
                     /locus_tag="PKY63_07130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPF00421.1"
                     /translation="MDPTIINDKILFRFSIFMAFLLCLPMVVTFVFEDFFSFLTGIYP
                     LTGKGLFGVLTAPFIHADWSHLAGNISALFILYLVLFNNFHPMAWVVILLSYIVPGFW
                     TWLFARAAWHVGASMMVYSLASFVFFAGIFSGHTRLMALSLFVVFMYGGIFWGIFPTL
                     PEISWEGHLSGFLLGILLAFFYRKDIRNLYPKKEYFEDEEPGGEDDNEEPNENGEQ"
     gene            19800..21383
                     /locus_tag="PKY63_07135"
     CDS             19800..21383
                     /locus_tag="PKY63_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPF00422.1"
                     /translation="MKLLFSLLLFSVTFFAFSQHDPAKTDEKFRTALQFMRFRYVDSV
                     DENHLTEVAIRKMLEELDPHSVYLSAEEIKQANEPLEGNFDGVGIQFQIIRDTINVVD
                     VIAGGPSEMVGIRAGDKIVSIDKQIATGKDVNNSFVLKHLRGPKGTQVVIAVARQGEP
                     KPIEFSVTRDKIPIYSIDAAYMITPEIGYIKLNKFAAKTMNEFAEAIKELKKNDFKSL
                     ILDLRDNSGGYLQTSIELADQFLPKDQLIVYTEGRSDKRVDYKATSRGAFEKGKLVIL
                     IDEGSASASEIVSGAMQDCDRALVIGRRSYGKGLVQRPFAFTDGSVMRLTVARYYTPS
                     GRCIQKSYEGGNDKYFDDLGERMKHGELVHPDSIKFPDSLRYKTKAGRIVYGGGGVMP
                     DVFIPFDTTKYSDYYVKLIRKNYFSTFTLDFLNANRTEMLSEYKTVEKFKEKYKISEK
                     FMKSFLDGAAAAGVEFDQSGFDQSKTIIETLLRAFIARSLYGSEAYYLIIGEIDDELQ
                     KAIECIQNPGMFEKYSIGY"
     gene            21474..22457
                     /locus_tag="PKY63_07140"
     CDS             21474..22457
                     /locus_tag="PKY63_07140"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPF00423.1"
                     /translation="MANLETSYLGLKLKSPIIAGSSGLSLKIENLVEMEKNGAGAIVL
                     KSLFEEQIRFEYAKQMSDATNLGYPEADDYIRQYTEHNELDKYLKLIADAKKAVSVPI
                     IASVNCYSDSDWVKFAEKLEKAGADAIELNIYHLPSDERINADAIEEQYAAIIDHVTA
                     NTNIPVAVKLSSYFTDLAHFVVKLSWKKPQGIVLFNRPFSPDINITKQSIVGTGVFSH
                     KDEISLPLRWIALLSDKVQTDLCSSTGVHDGEGVVKMLLVGAKAVQVTSALYQKGIAE
                     ISKMNAFLSDWMDKNNYKSIDDFRGKMSYKAHSNPAAFERVQFMKYYSGIE"
BASE COUNT         6056 a         4633 c         4819 g         6903 t
ORIGIN      
        1 aaatcgtttt tcggcttcca cgatgtagtg caaatataaa taattatttt aatcgaagct
       61 tcgcagtttt ttttaaatgc ctgaggctct ctaaacctct caaccttaat ctttgtctta
      121 acctcagctg aaagctgctc aacctcaacc tcaaccttaa ccttaacctc aaccttaacc
      181 ttaacctcaa cctcaacctc aacctcaacc tcaaccttaa ccttaacctc aaccttaacc
      241 tcaaccccaa cctcaaccta actgactact gaccactcac tactcaccac tattctagaa
      301 gtcccaccag atcttcgctg ctaatgttac tactgatttt ccggttgata aaattattga
      361 acaaatccat tttcttttct tgcaaagcca taattttttc ttcaatgctg ccttctgtga
      421 tgaatttgta tgaaattact tttttatttt gtccgatacg gtgtgtgcgg ttgatggctt
      481 gttgttccac tgccggattc caccagggat caagcagaaa aacaaaatcg gcagcggtaa
      541 gattcagtcc cacaccaccg gctttgagcg atatcagaaa aatcggatag tcgtaccctt
      601 tctgaaattg cctgatcatg ttctggcgtt gaatggtgtt tgtctggccc gtaagcatca
      661 gaaaaggaat gccctcatct tcgagccagg atgcgtataa aaccaggtgt tttacaaatt
      721 gcgaaaagat aagtaacttg tgtccaccgc tcagaacttt acgtatgctt tcagtaacct
      781 gatcaaattt gcccgatccg ccgggataat ccttctcagt aatgcccggg tggttggcca
      841 gcaatcgcat gcgcatcagt cccgacaaaa taatcatata tgccttatcg gttccgtatt
      901 tttttgtgct ttctataata taattgcgca cttccgattt ccgcttttcg taaatctcga
      961 gctgttcggg ggtcatcgga cagaaatgaa ttttttccgt gagttctgga agttcttttg
     1021 ctacttcgtt tttcgttcga cgcaaaataa aaggattgat aatctgacgg aattttttct
     1081 ccgacgattt tgagcctctt ttttctatgg gctgatagaa gcggtcgcga aaaaatttta
     1141 aatcgcccaa tagtccggga ttaacgaatt gcaactgcga ccacaaatcg atcagactgt
     1201 tttcaaccgg agttcctgtg agcacaaaac gttgttcggc attgagttta gatactgctg
     1261 caaaaatttt actctccgga ttttttatgt attgactttc atcgagaaaa acaaagcgga
     1321 aagggaattt actgagtttt tcatggtcgt tacgtaccgt gccataggtg gttagcacca
     1381 gatcatattt ttcaacctgt ttttgattga aacgtcgtcc aagcccggtg taattcagaa
     1441 atttcaattc cggcgcagtg gttctgatct cgttttccca gttgtgtacc agcgaaagcg
     1501 gcatgatgat aagcgaagtt tttgcatgtg gaattgttgt ttcattgctg aataagtcca
     1561 actgtgtttt ttgcctgaat tcttttgaaa tttctctcat tttcagcaga aaggcaagca
     1621 cctgaatggt ttttccaagt cccatatcgt cgctgaggca accaccaaaa ttgtttttat
     1681 acaaaaacat aaaccagctc attccgcgca gctgataagg gcgcaattcc tttttcaatc
     1741 ctttgggtgc atcctcaatc tgaaaatcgc ctttacccgc tgtttcaata aaatcttcca
     1801 cgcggttgtt tccttccagt atgttgctga taaaactgta ctggcttttt tttacgcgta
     1861 cattttctcc ttcgggtttg cttatcatag ccagatcacg gaacctggca aaccattctt
     1921 cgggcagtat ggctatggat ccatcgggca gcacaagttc acggttgttt tccagaatgt
     1981 ttttccgcaa ggcactgaat ggaattttaa actcatcgaa ctgaacaaat attttcaggt
     2041 caaaccagtc attgtcttct gtaaactgtt gcatcagctc gattcggcca gtgaagaatt
     2101 ttttgtcgag taactgggca atatcaaatc ctttttcttg taacgtggcc gaatactcat
     2161 ttatgtagtt gatgatatcg tacatttttt cagatgcact tttgttctga tcatcaatct
     2221 gcactttcag tggcaggaaa tacgaagcgt aggcaggtac cagtccatta tcctgcagcc
     2281 attcaactac ggttgtttca aactcattgt tgcgccgtat gatgtcgaag ctaaaattgt
     2341 tttctttttt ttgtaatgca acatgtgatt ggatgtccga tccgtattca aattcaacat
     2401 tgtcgtatct gaaacgcaac atgagcatga tgtcgttttg caacgatgtt tcgatgcgca
     2461 gaaccggaac cgggttttca ccgaaatgac tgaaaggtat tcccgtaagt tcaaatgggt
     2521 atgtggcaat tatttgccgc acaaattttt tcagataggg ctcaacctgc actgcctcta
     2581 caatcacctg atccttggtc agaaatggtt gaattttttt cccgtcaaca ttgtcggcta
     2641 cctgaataaa tttgttatcc ataataagcc agcatggatc atagttcagg attagggtgt
     2701 ttttatccgt cagctttact ttttttccgg ccagttctgt ttgaatggaa tactgtatgg
     2761 tgccatcaat gtatgcgaaa cggaatttta caactagttt ttcggattgc ggaatgattg
     2821 ttttttcgct gataatatcc tgctgatcgc ctttgaaata gatgggaatc tgattgtcag
     2881 ccagaatact gatggccatg tcggttttat tccgcaaatg cttaatgaca cgacgcataa
     2941 gattgttgtc gcgctttact tctttttcaa aatctattgt acggaatcga ttgtgcccta
     3001 caatgcgcgc cactgcttcg tttttacatt catcaagaat agtgagcacc atatattcgg
     3061 cgctgttgta atcagtggtg taatgattga tggtgctggc tgccaccctg atatgattgt
     3121 aagcaaacat gccgtttttc ataatttgca cggcaaatgg ctccagacgc cagttgtagg
     3181 tggctgagtt cacaatggca aataccaatt tgtatttttc ggcgtctgtt tgattcacgg
     3241 aagtgtaaat aagagcaaaa ttaatattat tgtaannnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnngatgc tgatcagcga attctcgggc ttaagattta ttcgtcagac
     3421 aacgcagcgg cattgactca ggtgtctgtg aaaatgactg gaactactga tatcaacgat
     3481 gtatcagctg ttaaattgta ttttaccgct gactcaaaca aattttttac cggtaatgga
     3541 agttccttgc tcgattcaga aaattcatcg accggtgcaa tttttctgag ctgcaatcaa
     3601 catctgatgg ccgataccaa ttatttctgg attgtctatg atgtgagtgc tgctgcgtcc
     3661 gaaggtcatc agctggatgc tgtagttgat tcagttgttg ttgatggtga tgctttttct
     3721 gttgcagatc agagtggtgc cagaacaatt ttgctgacca atacacttct tttcagttgt
     3781 aatgatgcgg gttcaaagca ttaccgcatt ccggccatta taacagcgcc cgacggatca
     3841 attgtaacag ccaccgataa gcgttggaat aattcggccg atttaccaaa cgacatcgat
     3901 ataattatcc gtcgcagtac ggacaaaggt gaaacatggt cggcgccgct caccattgcc
     3961 ggcggcgata caacgcttgg tttcggcgat gctgccttgt gtgtggataa aacaaatgga
     4021 aatattattt gtttgatggt ttcaggtccg ggactttggg gcgcaacagc tgcaaatccg
     4081 ttgcgtataa tgaaatgtgt cagcaccgat aatggactca catggtcacc gccggttgat
     4141 attaccgata tgatttatgg tgccggatgc agcaacattc ttacaaaaac atggtggtcg
     4201 gcttttgtag catcaggaag ttttgttcag tttagtaatg gtcgtttggc cgctgtaatt
     4261 acggtacgca gaacatcagc atactctatt gataattata tgatttattc agatgatcat
     4321 ggagttacct ggaacatcag taccaatcag gccgaagctg acggcgatga agctaaactt
     4381 atagagttgg ctgacagcac cgttttgatg agtatccgca atacaggata tcgcagattt
     4441 aacacatcgg cagattttgg attgacctgg ggaaatgctt attcagaaga tgaaataact
     4501 gatcccaatt gtaatggcga tatactgcga tacacgtgcg tgaacgatgg ctatctgaaa
     4561 aaccgcatcc tgcattcgat tccatttgct ttgaaccgat ctaacgtttc tgttctctta
     4621 agctacaatg aaggaaattc atggcccgtg cgaaaaacaa tctattcagg tccgtctgcg
     4681 tattcatcgc tttgtgtgct gcccgatggt actatcggaa tttattacga agtgggtgaa
     4741 tccgaaattt atcagatgta tttttcacgt ttctcacttg attggcttac cgacggagcc
     4801 gatcatttca gctctgcaat aaagtaattt agccttagac ccaaccttca atcgttagta
     4861 ttatataggt tgacttcaca gctttgtaaa ggtggagttt atctgactgg caatacagat
     4921 gtcgggaaat ttattcttac atttgccaaa aatgggggaa ttttaaatga ataaaatcat
     4981 tgttttatat gattcgggct ttggaaacac caaacaaata gcacaagcca ttgttgaagg
     5041 gatgggcgat tcggcttcat tgctgcatgt gtcggaattc accagagagc atttgaataa
     5101 tttgtctttc attgttgcag gcagtcctat caatggtttt atgccgaccg aaaaaatgca
     5161 ggccgcattg aaaactatac ctgccggtgc tttgaaaggt ataaaagccg catcgtttga
     5221 tacgcgcatg catgtgtgga ttcacggcga tgctgcaaag aaaatttcga aacggctggc
     5281 tgctgccggg gccgaaatca ttgctgttcc tgcttttttt catgtcaccg gaaaagaagg
     5341 tcctctcgct gaaggcgaga ctgagcgcgc tatagcgtgg ggtagaaaat tagttgaaag
     5401 tttttaaagt tgaaagtttt taaaggtgac gttggaaata ttcaggttag ataattctcc
     5461 aattcgcctt tccatttcac aaagccatca atcagttcag agtagtcgtg ccggatgcgc
     5521 tgaaagctgt cttcattggc gttgaggtag gcgattttcc cctgcacttt tattacattg
     5581 ataatgtctt caatttttcg caatgtcatt ttcagcatgc tcaaatcctt gtcggtatgc
     5641 catatgtaag tggcttcttt ggtgtcgatg gtttcccaaa taatgtgata atgccgttcg
     5701 ccttcaagca gaaacagaaa cgaaaacggc ttcagcacaa aacgaagctt catgatatga
     5761 aaggcatgct gtgatgaaag aaaccgcaaa tgtttatagt gttttgtttg tgtaatcgaa
     5821 atcagatctt cgaataattc attttcgttt tcataaaatg catttgattg aagccggctc
     5881 tcagtgagcg attcgaaata ctcatccatt gtaaataatg tcttgtcgac ttcggcattg
     5941 agttttttct ttgtggtatc gcgcacaaat tcgaagcgca cattgtcaat cattttttta
     6001 tcaatagaag caatctcgtt tgattgaaca cgcatacaag taatggtatc gttttcaatt
     6061 tcgacatgcg caaccacatg aatgtttttt gttttaagtg cgttcgaaaa ataatttttc
     6121 actgcatcga attcttcacg cagctcgtag ttttcgatgg tgagttcaat tggtttgtga
     6181 tagtcgcgga atgtgtgatt gaatgcaact ccgccgaagc gaaaacacaa ttcgttgatt
     6241 ggtactgaga aagtttcatc cagaatgagc atcctttttt gtgccggcgc ctgaatgtcc
     6301 agcagaattt cctgttgcat ctcttctgaa tacagctctg agatgggctc attaatgaat
     6361 ccttttgtcg gttcgtttcc tctaatggtt gcgagcaatc cttcggtatc gatgctttta
     6421 aaagtaattt gcgaaattgt ggaaagttgt tcttttattc gcagctgttg tggcggatat
     6481 tctgccagac ctgtcttgta tgacaataca gcaacaaaaa tcctgttttc cgattcacgg
     6541 taattaagca cttttactct ccaatgagcc ggtgctttga aatttttggc cacaggcgga
     6601 aaactaagct gctcaaaaga caaggggctc ccttcgcaca gataggcctt gtcttcaatg
     6661 aaatttatta tcagctcagt ttctttcatt cattcgggtt gtatttcgaa atacaaaagt
     6721 aatcatgaat tttgattttt tgattgaaat gaaattgtct atttttgagg aataaatatg
     6781 atgttcatga gcacccgaat cctgttaatt gacgatgatg aaaattatgt tgcaaaactc
     6841 agcgccgacg ctctgaaagc cggttttgaa attgtatctg atgataatct cgaaagcggt
     6901 ataatccggc ttcgctccga ccgccgcatt gcagctgtta ttcttgatgg ccgttgccct
     6961 ttgtctgcat cgggcaatca gccggcaaag ctgagttttg taacccatgc actacagcag
     7021 attcgcgaac ttgaagatga atataaccgt gcgattcctt tttgtataaa tacgaatacc
     7081 cctgaagaat ttctcgaaga gtatgaaggc atagcgaggg ttttcaaaaa agattccgaa
     7141 catccggaac tgtttgccca tctgcgcgat ctcattaaag cattgcccga gaccagagtc
     7201 agagaagaat ttggcggggt gcttacaaaa ttcagcaacc gcttcgatga tgaaacaacg
     7261 gaattgctga tggatgtttt gcttgcgttg gaatcagccg ataaagctac tataataaaa
     7321 catctcggag tgttgcgatt gttgctcgaa tcacttattg acgaagtttg tcaggaaaag
     7381 ctgcatcgtc cggttgaagc atatatgcat aaggaaggga gccggacacg ccgtattctg
     7441 gatgatttga ggcgacgtgt aattccgata gaactgtatg attcggcaat acagatttat
     7501 cgcatttgca gccgttatgg taatcatgct gacccggtgg ctccaggtgc catcgtgctg
     7561 agtcccacac gctacactta ccagcggctg gtgtatggat tgctggagtt gctggagtat
     7621 ttgctggcgt gaggatttac ctattcataa acactatccg gcaggccgtt ttgtccaacg
     7681 ggattatgac ttcgcttcgc tcgtcatgat cccgtnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnngggat tatgacttcg cttcgctcgt catgatcccg taatggggga
     7861 ggcttcaccc attcataaac actatccggc aggccggata gtgtttattc atttatgcct
     7921 gcgccttatg aaagctcgct ttcacggcgc cggcataaat gaaaacagcc acgcttgcgc
     7981 atggctgttt atgaatgggt ggcggagagc gagggattcg aacccccgga cccttgcggg
     8041 tcaacggttt tcaagaccgc cgcgatcgac cactctgcca gctctccgcg acaaaagtac
     8101 aaataatatt ttaatccaat gcaaccgatt ttaccccttt ttgttaattg cttgattgtc
     8161 cggccgtaaa aaattgcacc gttaacttat ctgaactgca tcattgctga ctcacttggc
     8221 attttactca attttgatta tatttgtact gccaaaaatc aaccacatga aaaagctttt
     8281 attgcttctg gttatcggtt tctgcaccat caatatttat gctgcaccaa agaaactgaa
     8341 tgtttacatt tcctattgca cgttctacag cccgcaggat ggcccctatg ttgaaacata
     8401 tctggcttat aacggcaatt cagtcaatta tcagaaaaat gaaaacggtc ttttgcaggc
     8461 aaaactcgaa gttcttatcc ttttcaagca gaatgattcc ataaaagcat ttcgcaaata
     8521 caatctgcac agtcctgaaa tgaaggacac gacgggtttc aatctgctat ttcatgatga
     8581 gcagcgcatc agtgttccga acggcgatta ctcgatggaa atttcgataa aggatgctta
     8641 tggtactgaa cccgcgattg taatagcaga gccggttacc atagaatata caaataagga
     8701 aattcagatt tcaggcattg aattgttgca gtcggccgaa aagactgtta cgcctggagc
     8761 cacatccaaa gctgggtttg acttgttgcc tttgccgctg aatatttatc ccacttcggt
     8821 aacgaagatt ttgttttata ccgaaatgta caatatgctt tcgattctgg gcgatagcag
     8881 taaatatctt ttccggactt atatcgagga aatgcagacc agtcgtaaga ttccgggact
     8941 tttgtttcag aaaaggatgg attccaaaaa tacggaagct gttattcatg aatttgatat
     9001 cacttcgttg ccaacgggtt catatttcct tgttattgaa attaaagaca gagaaaatat
     9061 gctcatggcg gccaatcagg tttattttga aagatggaat gaccgcgaac aggatatgat
     9121 gattgttgat gaaagttatg ttgcagcttc gtttgtcagc cgcattacgc agattgattc
     9181 tttgaaagaa tatattcgtt ctatcagacc tatttccact caaatggaaa aagtgtttgt
     9241 cgataacaat atcaaaaccg ctgaccttgc ggatttacag cgtttcttct taaatttctg
     9301 gatgcaacgt gatgagatgg caccagaagc agcatggatt gaatattaca agcaggtaat
     9361 taaggtgaat gctaatttcg gaacccgtat ccgaaaaggt tatgaaacgg atcgtggacg
     9421 tgtttatctg atgtatggcg agcccaattc cattacgtcc gttccgcatg aacctgctgc
     9481 atatccgtat gaaatatggc attattacaa ggttttgaat caatcaaacc gtaaatttgt
     9541 tttctgtaat gcagatcttg taaccaacga ttacgagctt attcatagtg atgccatagg
     9601 cgaaatttat gatgcaaact ggaaatcaaa attactgaag cgtaatttca ccacaaatag
     9661 tctcgatgaa cagacgcccg attttggctg gggaagcaag gttgatgact atttcagcac
     9721 tccacattga tttaagaaat cagatttgag atttttgatt ttagatctta gatgagattt
     9781 tgatgtgatt gatgatttga aatttgattt taagactggg aattttcaat cttcaaacta
     9841 ctgtaattcc ttcaaaaatc acatatctaa cgtctaatat ctaaaatcat tccgggagca
     9901 atggtgaaac agcattcagc cgcaccaccc tttcttcggg cacccagccc tgcgtgccgt
     9961 cgggagtggt gatacgcagc cacggcgttt tgatttccag tatatgtact ttaatgcccg
    10021 gttgtatcat cgcaatccct gtcccatcct tatcaggaga acttcgcaat tgcacggctt
    10081 catctacaac aatggcattt ttcgaatgat gtatgttgta atttccgctg atactctgcg
    10141 ccagcgccaa tagtaaaaca acaaataata ttacagatgt gagaaaaaat atttttctat
    10201 ttatattgga tgcggaagtc agaaacagta ttaatgcaac gagaaatact acaagaacga
    10261 ttatgaaaaa gtttgtccac cattgccacg acatcagcgc agaaagccag ttccaggcca
    10321 tgatgaaaaa attttcaggc acgatttcct gatttcccaa tgccgtttta tttacaaaag
    10381 caagattggc tgcagcatct tcgtgtgagg cattgagaag cagacatttt tcgtagcacc
    10441 agattgccgc ggctgcattg ttgttgcgat acaaagcgtt accaagatta taatacactt
    10501 catctttgat gtaaccgctc cgtaagagtt cgttgtattg aacaatagat tcttcgtatt
    10561 ttttttcgtt gtacaatgca ttggcacgtg aaaacttttc tttcggagtc tggctctgag
    10621 cgatggatgc aacagaaaca aaccacaaag ttgcgaatag aatattactc agtgttttca
    10681 ttttgctgcg tgtgtttgaa cttttacaat caggtctgct gatttttcat acaattcttt
    10741 ctgactcatg cggcccgctg ccggagcgaa tctcgtaaat tcgcattcat caagaatttt
    10801 taaaaattct tctgaaattt ctgcaggcac attgctgcga aggagaattt ctctggcgtt
    10861 tgaaagactc agttctgaaa acgggattgt aaatttatcg gttagataga gccagagcgc
    10921 ttttgataat tcagtataat actcctcttc ttttccttgg tcgagcaatg atttggcagt
    10981 tttcagacga cgctttgcct gacgcatggc cattcgcatg cggtattccg aaacatttgc
    11041 tttaagttgt atgcgtttcc ggaaatacca cagtataagt gcggccacta aaatcagcac
    11101 tgcaactacg atccagtaga gaactgaaaa gctgaaagtt tttgaactca gcatcagaaa
    11161 aggaatattc tcatgaatgt gcctaatgtc ttcgttcaat attttctcgg aactaacatt
    11221 cgatccgttg ccttttagta cttttatttt gtattccggc aattcaaccg actggtattg
    11281 ccctgtagat ggattgaaaa atgaaaatgc agatggcgtt actgtgaaat cgccgggaac
    11341 tgtgggctga agcagaaatt caaaggtcct cgtaccactg attccatcac tcgttttata
    11401 aaaatcatca ataatgtctg gatcaaacca ctcgaaactt tcgggaatac cgggctctgt
    11461 atattctatc aatggcaggt taccattgcc atttactttc attcgaagca ctactgcatc
    11521 tccggtaaaa catttattgc gatccacttc tgtttcgact tcgaaatttc cgactgcacc
    11581 attgaatgac tcaggtttcc ctttctcagg cagagcaata accgttattg aaaaaggatt
    11641 gctgcggatt tttcgtttct cggttttcac accgaagccg gcaaatggat caatgggaga
    11701 tgagccaaag ggatctttga aaaattgatc aaaaattgaa aatgggttgt agttttgttg
    11761 atttggaatt tgcactatgg cctccagttc aagcgacgga atcgagagcg tgcccgtttt
    11821 ttgcgctatc agcgccactt tgcgaatttc gcccacaata taccttttac cttcgatggt
    11881 ttcttcccat gtttttgcag cagttttagt atcagttagt tcttcggtcc aaaatccgtc
    11941 catcgatggt gttttctcaa tgccgtattg cgagatgtcg tagcgggtgt ataatctgta
    12001 agaaacagta atttgttctc cttcataaac tctcgatttg gagggaactg cagtcaggaa
    12061 aacttcctta ccgcccgatg tagattgttt tggctgattt ttatcgggct ggacggtatt
    12121 gttgtttgcc tgtccctgag attttacagc atttccgctg tttgtaacgg tgattgaaat
    12181 actgtttgag ctataggtct gtcctttcac tttcacccgg gctgggccga tggtgtacgt
    12241 tcctgtagat ttcggaacca atgtgaaaat ccatttcgac tcaccgggac tgctttgtac
    12301 gagctgtccg ttcacaataa tctgcatgcc gccaccaccg gaatacgacg actgaccaac
    12361 aacttgaaac ccttcaaacg atggtttggt aaacgtttcg gcattgcccc cagatagcga
    12421 ataagtaact tttacttgtg agttgaccgg cactgttgag taatcgctgg acgcagaaaa
    12481 ctttatctga gccgatattg tcagactgaa tattatgcaa accaaggtga aaattgtctt
    12541 catattctac cagtctttgt atccgtttgt tgcaccctgt ccttccttca caggttgagc
    12601 atgtatttct ctttgtgttt gtttttcttt attttgcaaa gctttcagca tttgatctgc
    12661 ctgaccggaa ctgatttcat ttttttcctg ttcctgtttt tgttgttgat ttttgttctg
    12721 gtctttattc tgatctttgt ttttatcctg attttgctga ttttgctgat tttgctgatc
    12781 ttgctgatct tgtttatttt gttgatcctg attatcctgc tggttctgtt ggttttgctg
    12841 attctgctgt tgttttttca gcatttgcaa tgcgtatgaa agattgtaac gggcgtcttc
    12901 atcggaagga ttgagccgga gcgcattttt ataggcaata acactttgct catagttttc
    12961 ctcctgcata tatgtgttcc cgatgttatg atagagacgg ctctgggtgc ggttatccaa
    13021 gctgtcattc agtaagctgc tgtaggtttg tctggctccc tcaaagtctc cgtttcggta
    13081 taaactattt gcaagattgt agcgggcctg aaagtcatat tctgtttttt gctccagact
    13141 tttgcgatac aaacctgccg ctttatcaaa ttcttttttt cgataggcct tgttgccttc
    13201 aattacattg tgaagcgaat tctgcgcgca gactactgtt gaggtaataa tagcaagagt
    13261 gaatgccaag agtattttca tcatacagtt ctcctttcaa tgaagttttt aaagacatcc
    13321 tgccatttca tgcggcgttc catcagcaca aaatcaatga gcaacagcag aaatgccgga
    13381 atgagcagcc aaagatataa tgatttataa tcagaaaaaa tcatcgatct gttttcggtt
    13441 ttttctgctt ttctcaattc atccataatg ctttcagctg catttccaag atttttggtg
    13501 tcaatgaaca gtcctccggt ttgtgtggcc agacctttca gcaaagcttc atccggcttc
    13561 gaaatcacag tgtttcctcc ttcatctttt ttatatcctg ttctgacaga gttgttataa
    13621 accgggatgg ggcctccttt ttcagtacca atggcaatgg tatgaacagg aattcttctt
    13681 ttctgcaatt caccgataat cggctcagga ttgccttcca gattttcacc atcactgagt
    13741 aaaacgatgg ccggatattt tgccttactg ctgtcgatgc ttatcaaagc tgttgtcagt
    13801 gctttttcga tatgtgttcc ctgccggctg atgagctccg gatcgaggtt ccggacaaac
    13861 atcgaaacgg cagtctgatc gatggtcatg ggcaattgaa taaatgcatc gccggcaaaa
    13921 acgatgagcc ctgcgcgatc cgatcccatg cggtcaatca tgccaagcat aatttgtttt
    13981 actttgctca gtcggtttgg tttgatgtct tcgcagcgca tcgaattgct gatgtcaacc
    14041 acaaactgaa tgtcgctccc ttttgattga atttgtttgt ccactttttt cccggccagt
    14101 ggattggcga gtgttacaat catgaaagcc gctgctgaag ctacaagaat gaatttcgtc
    14161 aatggtttgt tttttgaaaa cccgggaatg ctgagccgta gattattttc tgaaaacatt
    14221 tggccgagct gtttctttct tgcgaaatag tacagcataa acaaacctgt tgccagcagc
    14281 agagtgctta accaccagag atattgcgga tgttcaaatc tgtacataat gattatggta
    14341 taattcgtaa aacagtgtaa cgaagcagta attcaaaagc aagaagcaca agggcaatca
    14401 gaaccggaat aaaaaacaaa tcatgttttt ccgacattcg ggcaacttta attcttgttt
    14461 tttccatttc atcgatttcg gcataaatgg attcaagcga tttgttgtct atggctctga
    14521 aatattttcc accggtcatg gctgccattt cctgcagcat gggttcatcc agtcgcacat
    14581 ctacatattc ataggacgta cccatgatgg ttttgacagg atatggtgct ttgccagttg
    14641 taccaacacc gaccgtgtat agacggattc catacagttt ggccatggaa gcggagttga
    14701 gtggatcaac aaaaccactg ttgttgattc cgtcggtaag cagaataatg actttgctga
    14761 tggcggagct gtaacgcagg cggtcaaccg caattccaac tgcatcgccg atggcagtac
    14821 cgtcacgcac aatgccgttt tgaatctgca taagcaattg ccgtaaaact gcatgatcgg
    14881 ctgtaagcgg acattgtgtg aaagctgcac cgctgaatgc aaccaatcct atccggtcgt
    14941 cgggtcgtcc ggcaataaag tcctgagcta ctttttttgc agcatcaagc cggttgggct
    15001 tcaggtcttc tgccagcatg ctgcccgaaa catcattgac cagcataata tcgatccctt
    15061 caatgttata gtcgtcatga aaactgtacg attgcggtcc ggctatggct acaataatga
    15121 atgcaatggc tgcgaatctg atagcaggca gcaaatggaa cattctttcg cggaatgtct
    15181 tcggcgcatt ttcaatggct ttcagcgatg atgcatgaat tgcatgacgc gattgcctga
    15241 aatttctgat ataccaccag atcatcaatg gtataatcga catcagcaga aatgcccacg
    15301 gatgctcaaa agaaagatca tttatccatt ccattgttgt cggtgtttgt ttcaactggt
    15361 cttgtgtcaa ttacaaattt gatggcatcg ctcaccgatt tttcgtattg tcccggctcc
    15421 ggacggaaac gtgcaaattt ggccatatca gccacgttca gaattcctct cagcgtgtgg
    15481 atgctttcag ccggaagttc agcttttgtt actgcttcga ttaattcgga tgataacatt
    15541 tcttcggctt gaataccacg tgaatagatc agatatttcc gtaaaatctc aacaagctcg
    15601 gtgtaaaagt cttttggtga aacgctaaac catgagcgcg attcctgtat tttccgaagt
    15661 gctgtcagtg cggcttcggt gggattgacc cagacaattt cctcaaccgg aatcataggt
    15721 ttctttttac gcagacggaa ataaaaatac actcctgcag ctgcaatcaa tactcctgca
    15781 agtaggtacc agagccaatt aaatgatgtt ttgccttcga cttcgtaaat atccttgatg
    15841 tcttttacgg ctttggttgt gtcaaccgga actgttgtgc aaaaaagcat cagcgagtca
    15901 ctgagaattt ccaccgtatc attgtccttc aggaaaagcg ctgacagcgc gggaatagcg
    15961 aagtatccac tgtcgtatgc agagaccgat aattgttgtt tgtatatatc gttcgatcct
    16021 tttttttcgg tgataatatc ttttttcttc acaacttcga ttcctgacgg cattgaatca
    16081 gggagcatcg gcatttgcag aactttggtt cctgtgggta aagaaagctg ccattgaaaa
    16141 acggacaatt cgccaacgct gatggaatca cgactgagcg atactttaat gtcgttttga
    16201 gcatgtgaaa acgaagttat taaaataaaa atcagtagag ccgccggttt tctcatatcc
    16261 tttttcctct ttttttgaaa agtttggcca atggttttac gaaatcttcg tgcgttttga
    16321 aatcaaccac atcgatgttg aatttggcag cagtttgtgt aagatattgc tgttgctgat
    16381 tccaccatga attgtacatt cttgctgtgg cgccattgga gtcaattaca cgtagttggc
    16441 cggtttcgga atcctgcata agaacaagtc cggagcccgg aaccgaagat tctattttat
    16501 caattacacg cagtgccacc agatcgtgtt tgcgggcagt gatggcaaag gcgcgctcgt
    16561 agccattgtc gatgaaatcg gaaatcagaa aaacaatgcc gcgacggttt acaacacggt
    16621 tgaaatactc gagtgcagcc gtaatattgg taccttgccc ttcaggttta aaagtaatca
    16681 gttcgcgtat gatgcgtaaa atgtgttgtt gtcctttttt cggtggaatg aatttttcaa
    16741 ttttatcgct gaagaaaatc accccgactt tatcgttgtt gcgcaacgct gaaaaagcaa
    16801 gtgttccagc cagttctgca atgcgttcat tttttgaaac agcggttgac ccaatgttgt
    16861 ttgagccgct gacatcgatg agcagaatga ctgtgagttc tcgctcttct tcaaatactt
    16921 taataaatgg atgattgaac cgggcggtca cattccagtc gatgctgcgc atatcgtcgc
    16981 cgtagccgta ttcacgcact tcgctgaacg tcatgccgcg tcccttgaaa gcgctgtggt
    17041 acgaacccgc cagcacttcg tcggtaagcc gtcgtgtttt gatttcaatc cggcgtactt
    17101 ttttcgaaag ctgaaaatta tcgttttcca tcacggcacc tgaactgcgt taagaatgtc
    17161 aatgataatc tggtcagttg taatgttctc ggcttcggct tcgtagctga gtccaatgcg
    17221 gtgacgcata acatcgggtg caacggcgcg gatgtcttcg ggcagcacat agccgcggtg
    17281 attgagcagt gcataagctt ttgatgccaa cgccatattg atgccagctc gtggcgatgc
    17341 tccataattg atcatgtctt tgtattttac taagccataa cgtgcaggga agcggcttgc
    17401 aaacacaata tcggtaatat agttttcgat tttttcatcc agataaatgt cgtgcaccag
    17461 tttgcgtgca tgcagaatct gttcaacaga ggccacttta ttgggcaccg gatattgcgg
    17521 agccaggttc tgacgtataa tcagtttttc ttcttctttt tccggatagc tcaccagcac
    17581 tttcatcatg aaacggtcaa cctgtgcttc aggcagcgga taggttcctt cctgctcaat
    17641 ggggttttga gttgccatga caagaaaggg ctcttcgagt ttgtaagtat tttcgccgat
    17701 ggtgacctga cgttcctgca tagcttcgag caaagcgctc tgtaccttgg ccggagagcg
    17761 gttgatttcg tcggccagaa taaaatgaga aaagaccggc ccttttttta cattgaactg
    17821 ttctgacttc gggctgtaga tcatggttcc aatcaagtcg gcaggcagca ggtcgggggt
    17881 gaactgaatg cggctgaact tggcgtcgat ggtcgatgcc agcgatttga tggccagtgt
    17941 ttttgccagg ccgggaacac cttcgagtag tagatgcccg ttggcaaata aggcaatcag
    18001 gagacggtca accatgtttt tttgtccaac aatgcttttc cccaactcca tccggatgag
    18061 gtcgatgaat gcactttcgc gttgaatgcg ctcgttaagt tcgtgaatgt caacagtagg
    18121 cgtcattttc tgaatttttt ttgcggggtt ttacaatttt aaagccaatg cattataact
    18181 gacaaaattg cggattagta tatgtcaatg taaaaaaaat gtcagtcaaa ctgtcgtcag
    18241 attttgaaaa attgtttatt gagcaaaaat gcgggagtca gaatacattt gtcggaaata
    18301 cgacccataa aagtgaaaaa actgtcggaa atacgacccg aaaaatgtta aatcatgtcg
    18361 gaaatacgat ccaaaaaatg ttaaatcctg tcggaaatac gacccgaaga tcattcttaa
    18421 aatagataaa ccaccaaaaa acttactctt tgtacgtatc cttacgctcc atttcgcggc
    18481 ggttgtcttt ttctttaagt gtttcgcgct tgtcgtacat tttctttcct ttggccagcg
    18541 caatttcaat cttcgcatag ccctcaggcg aaatgaaaag cagcagcgga acaatcgtca
    18601 gtccggtctc gcggattttg ttgtgtagtt tttttatttc cttgcggttc agcagcagct
    18661 tgcggtcgcg cttgggctcg tgattattgt aggttccata ggcatattcc gaaatattca
    18721 tattgcggat ataaacttcg ttttcattga taatgcaata cgcttcggca atagacgcct
    18781 ttcccgcacg gatgcttttg atttcggttc cctgcaatac aattccggcc gtatattttt
    18841 cgagaatgtt gtactcaaaa tacgcctttt tgttttttat ctgtatggtg tttttttcag
    18901 ccatttttat tcgggactgc aaagataata aatagtagtg agcggtgatc ggtgaatagt
    18961 gagcgggatt gcaacttttc agcaaccagc gttggcaagc attattgtaa actcaatatt
    19021 gtccaaacat ctttcattcg cagctcactg cttaccactc actgctcact ggtcaccact
    19081 cattgctcac cattttcatt gggctcttca ttgtcatcct cgcccccggg ttcttcgtct
    19141 tcgaaatatt ctttttttgg atacagattc cggatgtctt tgcgatagaa aaatgccagt
    19201 aggattccca gcaaaaagcc gctcaggtgg ccttcccacg agatttcggg cagggtagga
    19261 aagatgcccc agaaaatgcc accgtacata aatactacaa aaagcgataa agccataagc
    19321 cgtgtgtgac cagaaaaaat accggcaaaa aacacaaatg aggcgagcga ataaaccatc
    19381 atactggcgc ccacatgcca tgcagcccgc gcaaaaagcc atgtccagaa accgggtaca
    19441 atgtagctga gtaaaatgac aacccaggcc atcggatgaa aattgttgaa cagcacaaga
    19501 tacagaatga acagagccga gatattacca gccagatgcg accagtctgc atgaataaat
    19561 ggtgctgtca gaacaccgaa taatcctttg cccgtgagtg gataaatgcc tgtcagaaat
    19621 gaaaaaaagt cttcaaaaac gaatgttaca accattggca ggcatagcaa aaaagccata
    19681 aaaatggaaa agcggaaaag tattttatcg tttatgattg tcgggtccat gaattattca
    19741 taagaagtca caaaataata taattttgta aagcaaaacc cgtttttaaa gaaaaaaaca
    19801 tgaaattact tttttcactt ctgctgtttt cagttacttt ttttgctttt tctcagcacg
    19861 atcctgctaa aaccgatgaa aaatttcgta ctgctcttca gttcatgaga ttcagatatg
    19921 ttgattctgt tgatgaaaac catttaacag aagttgctat cagaaaaatg ctcgaagagc
    19981 ttgatccgca tagtgtttat ttgtcggccg aagaaatcaa gcaggccaat gagccgttgg
    20041 aaggcaattt tgatggtgtt ggtattcagt ttcagattat ccgcgatacc atcaatgtgg
    20101 tggatgttat tgccggtggc cccagcgaaa tggttggcat ccgtgccggc gataaaattg
    20161 tatccattga taaacaaata gcaacgggaa aggatgttaa taatagtttt gtccttaaac
    20221 atttgcgtgg tccgaaaggg actcaggtgg tgattgctgt tgcgcgtcag ggtgaaccca
    20281 aaccgattga attcagcgtt acgcgcgata agattcctat ttacagtatt gatgctgctt
    20341 atatgattac accagagatc ggatacatta aactgaataa gtttgctgcc aaaacaatga
    20401 atgaatttgc tgaagctatt aaagaactga aaaaaaatga ttttaaatca ctgattcttg
    20461 atttgcgcga caacagcggt ggttatctgc agacgtcgat tgagttggcc gatcagtttt
    20521 tgccgaagga tcagttgatt gtttataccg aaggccgaag tgataaacgt gttgattaca
    20581 aggcaaccag ccgtggtgca tttgaaaaag gcaaattggt tattctgatt gatgagggta
    20641 gtgcatcggc cagcgaaatt gtatccggtg ccatgcagga ttgcgaccgt gcattggtga
    20701 ttggccgccg ttcgtatgga aaagggttgg ttcaacgccc ctttgcattc accgatggtt
    20761 ctgttatgcg tttaacggtg gctcgttatt acactcccag cggccgttgt atccagaaat
    20821 cttacgaagg aggcaatgat aaatattttg atgatctggg cgaacgaatg aaacacggcg
    20881 aattagtgca tcccgattcc attaaattcc ccgattcttt acgctataaa acaaaagccg
    20941 gccgtattgt ttatggtggc ggtggtgtga tgcccgatgt ttttattcct ttcgacacaa
    21001 ccaaatacag cgattattac gttaagctta ttcgtaaaaa ctatttcagt acgttcacat
    21061 tggattttct taatgccaac cggacagaaa tgctttcgga atataaaacg gtggagaaat
    21121 ttaaggaaaa gtataagata agcgaaaagt ttatgaaatc ttttttggat ggcgctgctg
    21181 ctgccggagt tgaatttgat cagtctggtt ttgaccagag taaaaccatt attgaaactt
    21241 tgttaagggc tttcattgca cgtagtcttt atggttcaga ggcctattat ctgattatcg
    21301 gagaaattga tgatgaattg caaaaagcaa tagaatgcat tcagaatccg ggcatgtttg
    21361 aaaaatattc cattggttat tagtcatatg acaaataaca aaatccttat gtagggaaaa
    21421 tatttttccg actaaaaact tattatattt gtcacctaaa aaacactacc atcatggcaa
    21481 atttagaaac aagctattta ggattaaaac tcaaatcgcc cattatagca ggcagttccg
    21541 gactgagtct taaaattgaa aatctggtag aaatggagaa aaatggcgct ggtgccatag
    21601 ttctcaaatc gcttttcgaa gagcagattc gttttgagta cgccaaacaa atgtctgatg
    21661 caacaaacct tggctatccc gaagctgatg attacataag acaatataca gagcacaacg
    21721 agcttgataa atatctgaaa ttgattgctg atgccaaaaa agctgtttct gtgccgatta
    21781 ttgccagtgt taattgttat tcggattcag attgggtgaa atttgccgaa aaactggaga
    21841 aggccggagc cgatgccatt gaactcaata tttatcactt gccttctgat gagcgcatta
    21901 atgccgatgc cattgaagaa cagtatgctg ccatcattga ccatgtaaca gccaatacca
    21961 atattccggt tgctgttaaa ctgagctctt attttaccga ccttgctcat tttgttgtga
    22021 aattgtcatg gaaaaagcca cagggcattg tcttgtttaa tcgccctttt tctcctgata
    22081 tcaatattac aaagcaaagt attgtcggta ccggcgtttt cagccataag gatgaaattt
    22141 cactgccgct cagatggatt gctttgttgt ccgacaaggt gcagaccgat ctgtgttcat
    22201 ccaccggcgt tcacgatggc gaaggagtgg tgaaaatgct gcttgtagga gccaaagctg
    22261 ttcaggttac ttcggcttta taccagaagg gaattgctga aatcagcaaa atgaatgcat
    22321 tcctgagcga ctggatggat aaaaataatt ataaatccat tgatgatttc cgtggtaaaa
    22381 tgtcatacaa agcacattca aatcctgccg ccttcgagcg tgtgcagttt atgaaatatt
    22441 attcggggat tgaataagat ttattaccga atttaaaaga atcattgaaa tcccggccga
    22501 ctcaccggga tttttttaat tcagcgattt cctgaagcag ctttaacaac aagcaagtgc
    22561 ttgcagtcag aaatttttat cgacaattat ttgaattata tgtgaaaaat g
//
LOCUS       DAOPKE010000044        22517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2437_length_22517_cov_12.484062, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000044 DAOPKE010000000
VERSION     DAOPKE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2437_length_22517_cov_12.484062"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKY63_07145"
     CDS             <1..474
                     /locus_tag="PKY63_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00424.1"
                     /translation="QTICNDESTTLDAGAGFTSYSWTGGATTQTLTVDGAVLGVGNHT
                     ITVTVTNSYTCSASNDIIITVEDCSGIETATLQIGNVYPNPASESISIAFAAEHNNLV
                     INLFDATGRLVSSQSLKNTTSGNVVVIPVSQLSAGVYSLQIVSEEGIQISIVVIE"
     gene            595..1311
                     /locus_tag="PKY63_07150"
     CDS             595..1311
                     /locus_tag="PKY63_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPF00425.1"
                     /translation="MNSIFLQELDPQIDTWDVILRLFLSFIAGLIIGIERGRHKQVLS
                     LRPHILIAVGSTLVMLISIYMPQVFGADKNYDPGRIAAQVVSGIGFLGAGAIIRMGVN
                     VKGLTTATTIWVASAIGLAIGAGFYLAALFAVVAILLVLIFIDHLEKNVFRESLYKVV
                     TVKFDGVEDRKEDVVAVLKKHQVRILTINTEKDFDKQQSRLYFQVHIAMHADTEKICN
                     QISDMFGPGHLKFVGFETME"
     gene            1320..2528
                     /locus_tag="PKY63_07155"
     CDS             1320..2528
                     /locus_tag="PKY63_07155"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPF00426.1"
                     /translation="MDSVREIYRIGYGPSSSHTMGPSRAAELFLKSAPQAKKYRVHLY
                     GSLALTGKGHLTDVAITGILYPSEVQFIWHTDEVLPEHPNGMIFEALDSDGKITNELK
                     IFSTGGGQISQHPGKEISRHLYPLHTMHDIMKWCDDNGTQLWEYVVQCEGESILSYLA
                     EVWQVMKSSVQSGIEHEGVLPGPLKLARKAASYHAKSQGFNPAVMRRGMVYTYALAVA
                     EENAGGGRVVTAPTCGSCGIVPAVLYLLHNYNDATEQRIIRALATAGLVGNLVKHNAS
                     ISGAMVGCQGEVGTACAMAAAAATQIFGGTITEVEYAAEMGMEHHLGLTCDPVMGLVQ
                     IPCIERNAFGAARALDASTFAILSDGRHRISFDEIVQTMKQTGLDMNADYRETSTGGL
                     AKVMKNRKKK"
     gene            2525..3808
                     /locus_tag="PKY63_07160"
     CDS             2525..3808
                     /locus_tag="PKY63_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00427.1"
                     /translation="MRKIIFAAFFFAVSTLSIAQTVVRIPIGKSSEPADAPVKLTLIA
                     RIQNYNEHAASDSDFLNKHIDSPKSVNILDSGNKFYIHSLEGCETLVFSLDSLKMLKV
                     IPHTFSQKDTALFHENTVLGYSFSYHGVVNSFSGKPVESCFTHNKKYLWVTYYRRSFD
                     LNAMQPSAVAIIDTECDSIVRVMPTGPLPKMVAVSPDGKYLAVTHWGDNTIGIIDVSS
                     SNPNDFHYVHHFVVDYRLSLKGDGNKPINRDSECGFCLRGTVFSPDGKYLLVGRMGGG
                     GIAFFDMDKMIYMGTLWGMKTNVRHLTIAGNDLLVSCTRPGFVQKAPIDSLIACMERK
                     QNNYSSWENAFVGIGVRTIDVSPDRKYIFSAINNGSKIAITDYTSMEIISTVSADSFP
                     VGLAVTEDGTKLIVTSQGRNGIGGGNSVMIFSVEY"
     gene            complement(3805..4035)
                     /locus_tag="PKY63_07165"
     CDS             complement(3805..4035)
                     /locus_tag="PKY63_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00428.1"
                     /translation="MKRFFSKYMLLLIVFVATMAMSSCSSSQFKNYKRYVKRKNSALG
                     YKSHYQKRLKRNTMPINRNYIIRNKRTSPSWR"
     regulatory      4803..5005
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5060..5803
                     /locus_tag="PKY63_07170"
     CDS             5060..5803
                     /locus_tag="PKY63_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00429.1"
                     /translation="MKHFFHSSLLWLALAMPFMSNAQFSMDDISFWVGSGSKKTVLVI
                     DFNDGANPQCYAWGYRFDGTSVTAEKMIADIDSADHALSMVVMSGFVNDITYLSHAGL
                     GGNPDYFSTFSGDGDTSNWVMNMGGSELLTDSMWFGMSYTPWDSSFNPLYKPGLPVAA
                     SPVNGFADIRIFNTIIYPNPVRDFFTINNYGNFDRMQIFDVNGKLVCEYGILEANPEY
                     SLNAVPKGFYYALFYNNNAVLHGELIIKE"
     gene            5800..6765
                     /locus_tag="PKY63_07175"
     CDS             5800..6765
                     /locus_tag="PKY63_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00430.1"
                     /translation="MKTIVFLFLIQLCFFAKAQYPPAAGQAGSTAVPHDSACFIDWAG
                     SCSIVRGFVNVSDTTVVAGSSNYASYGAESNVAGIADNVVVSLGDGGVADIFFNVPLA
                     DGSGFDFAVFENALTDLFLELAFVEVSSDGIHFFRIPSYSLTDTIVQTGGFGNTDPTK
                     IHNLAGKYRALFGTPFDIADAPDTALLRKDSITVVRLIDVVGSIDPVYASRDCEGRKI
                     NDPFPTPFESCGFDLDAVGVINNRSNTGFDEYQKIVHVYPDPASDVIYFNSSETERVF
                     IYSCSGHLMREYNGSEKSADVSGLDNGIYFLRTSEGKSSKFIVFH"
     gene            6765..8681
                     /locus_tag="PKY63_07180"
     CDS             6765..8681
                     /locus_tag="PKY63_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPF00431.1"
                     /translation="MKGLFFILFLLLGFLLNAQQQNDTIWLKPVDISAPADDSVFMIS
                     VSGKLIPADENLSIENALSRYSPVFFKTYSPGGIASISHRGFGASQTLFVWNGFALNQ
                     LTLGQPALNGMQVNENMHFGIVSGTLAASDFSGGLSSYIYVDDGFYRDSSLMRRLVIS
                     GGSFGAGSASFFSDYRIKKSRVSFELSGMNVVNNYRFHNNAEGAGVEQWPLQNRVNAS
                     YFGMTVKSNLLIPLKNGDFKFSLWAGSQFNESPAQLLSPQLSDNESQENRFVRMSGFV
                     PLLTAADAGLQIRFFFSADTFLYRNKQLSISDGTASQTLATRWNAYLRAGAGHEFKFE
                     YYPEFNRVLSENFSKPVLSTDQRLNLNYCLNRSDVVKAIIWTHFITRNFDELFPLPGL
                     SLSFMPGNSSKANWKRLKWLSVNAGIARNMRMPTMNDLFWEPGGNPYLKPEDAWMADM
                     AIVASSAFKKIKWDFKVMPFFSETFNLIRWTPDSLSSQWIASNVSESRQYGVEGVAAC
                     SYSWQQNTITGSMNVTRVFAQDMSGDKVFTLMYVPDKTMNSSVSFEHGIWSGGYEFHY
                     TGKRYSSSDNDRYMPEIFLHDVFFSVVKKIKSDQLSLSLRFNNIMNADYQYIAWYPMP
                     RRNFHVTLKWKWNE"
     gene            8674..9732
                     /locus_tag="PKY63_07185"
     CDS             8674..9732
                     /locus_tag="PKY63_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00432.1"
                     /translation="MNRWLLILLLALSLYSCTNIEFGEVNTLDNSPSSFHNGAFVINE
                     GLFNSGGGDVSWIDFSNGTISNEIFRSVNGFPPGDIPFHAALSGDNIFLTINNSAKVY
                     IINDDFEVVKIIEGIASPREICAGSGNTMFVSSLYKPYIYVIDAEQGILSDSIYTERP
                     VENLLLHAGKLWATHWSKLVSTQTNNALLVIDTTSMQLEDSVIIGVEPNSMGVDVNGF
                     IWVLCSGGFDQAEMAKIVVIDPLSHSIIRNIPFASSSDYPSALVMNAARDSLYFVNQH
                     IYKMAVSATSVPTTAWIQSSGNTFYRLAVNPTDNSLWVSDAGDYVHAGQVLHYSLSGN
                     LLQTFNAGVVPGYVVFRE"
     gene            complement(10371..11009)
                     /gene="nth"
                     /locus_tag="PKY63_07190"
     CDS             complement(10371..11009)
                     /gene="nth"
                     /locus_tag="PKY63_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPF00433.1"
                     /translation="MTRKQRLAKIIEIFQQNNPIPKTELDYKNPYELIVAVILSAQCT
                     DVRVNKITPDFFKTFPNAKTLAAATESAIYEKIRSCSYPNNKARNLKAMAEMLTGKYK
                     GKLPTDPEEMQQLPGVGRKTAHVLASVLFQQNVIAVDTHVQRVSVRLGLAPGATTPLA
                     VEKALVSIAPADVLHDLHHWLILHGRYVCKARKPLCGSCAIADFCSYPLREV"
     gene            complement(11019..11435)
                     /locus_tag="PKY63_07195"
     CDS             complement(11019..11435)
                     /locus_tag="PKY63_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPF00434.1"
                     /translation="MPKPIQLSEAASIGIHALVLISNSEHNFMNVNVIAEKTGASKNH
                     IAKVMQTLVKSGFVSSVRGPAGGFSLAMEPSDISMLDIYEAIEGKISIKTCPFDKPIC
                     PFDKCLMGGIFHKVTAELIEYLKSHKLSDMSNYHIK"
     gene            11611..12795
                     /locus_tag="PKY63_07200"
     CDS             11611..12795
                     /locus_tag="PKY63_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPF00435.1"
                     /translation="MKNVKDMGFNTQLVHAGDFEDAFGSAVIPIYQTSTFAFRNAQQG
                     ADRFAGKEKGFIYSRIGNPTIGALEDKLAVMENGFGGIATSSGMGAVSTVYLAMLSQG
                     DHMISSSAVYGPSRNIMENHFVRFGIESTYIDTSDLSLIEKSIKPNTKLLYIESPANP
                     TMTITDIKAASDLAHKHGIMVCVDNTFSSPALQNPLNLGADIVLHSLTKYINGHADIV
                     GGAVIAKTEETYKKLRAIMVGFGYNMDPHQAYMVIRGAKTLALRVERAQQSAMKVAEY
                     LESHPKIAWVKYPGLKSHPQHELAKKQSKGFGSMISFEMKGGYEAGVKLMNNVHLIML
                     AVSLGGVESLIQHPASMTHAGVSSEGKKAAFITDGLVRLSVGIEEVEDILNDLGHALE
                     MV"
     gene            12796..15075
                     /locus_tag="PKY63_07205"
     CDS             12796..15075
                     /locus_tag="PKY63_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPF00436.1"
                     /translation="MKKLIIIGLLFYCTGFVNAQKVAVVLSGGGSKGAAHIGVLRALE
                     DNQIPIDYIAGTSIGAIIGGLYASGWTTDQMEQLITSEQFLMWATGAIDEKYVYYFKQ
                     PEPDASWASFRVNADSIWSFDVPTNLVSSGQMDFIFMEIFAQANAVCKGDFDSLFVPF
                     RCIGANITDSKAEVFSGGNLVKSIRASMTYPLYFKPITIDGKMYMDGGMFNNFPADVA
                     LKDFDPDIIIGSKVAAENAVPDPDNIMSQLENIFMIETSYDLPCNSSVLIEPQIPTTN
                     VVDFSHSAEYIKLGYDAAMSKIPQIREFVIDSVSKDEMQLRRDTFNKRKPPLVFDEVE
                     VTGMNKMQRQYVLNYFSRRETVGIERTREDYYRLMTDHAIRTIYPMADYKDTSGSFTL
                     NLEVKKESNILLRFGGLVSSNPVNQAFAEVSYKYLQRNAATVRANMYAGRFYSSALLG
                     MRLDFPGHPQYALFGRIVYNQWDYFKTTSHFFEDKNPSYLIVNDYHWDCGVVVPARNK
                     GKVTLAGSAMIIDDEYFQDNYFTRTDIPDKTNFKAWSVSAEWERNTLNKKQYANKGTL
                     LHLRTGFVQGKENTVPGTTSVLSVDYSAHHSWFYMQFIYENYFSFMGRLRMGWYNDIY
                     FSNRDFFGSYTATMLFANPFQPLPEMKALFMPQYRAHQYAGAGLKSIFMIRDNFDIRG
                     EAYLCQPIRDITMGPSGIYATYGPWLRSRYFVASAGLVFHSPVGPIAITASYYDKYDE
                     PLMLSFYFGYILFNPKAVN"
     gene            15143..17092
                     /locus_tag="PKY63_07210"
     CDS             15143..17092
                     /locus_tag="PKY63_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPF00437.1"
                     /translation="MEEKKETPAENADSKLNESEVVQQEVNESTEVETNTPVAENEFA
                     HGEDSYVLEEADESPQEEEHITENHDLDYSKWDNYNLDEIKAEALNVIQQIEDLMQVG
                     QALKKLRELHNAQIEIEHQKLAELGEDDPEAHEHKTELDKKTGEFNAIWGIYQEKRKE
                     HFDNLVRQQEENFEKKLVLLDDLKKLIEEGEPLKNTFDEFKRIQEKWREIGMVPKDKS
                     NDLWLNYNHHVQMFLDKVKLNRELRDLDMKKNMEIKVELCEKAEELIFEKSLHESFKK
                     LQDLHRQWKETGPVPSDVKDQIWERFRNASEKIREIRISHYEELNKKLEANLEAKQAL
                     VDKARNIASAEINTMKEWNAATKEMNELFGLWRTIGPAPKENNDKVWTDFKGILDVFY
                     AARKEYFNAIRSELNENYNKKLNICLEAEALRESNDWKKTSNELIRLQNEWKKVGPVP
                     KVKSDVIWKRFRAACDEFFNRKKNYFENLSTVEAENLQKKMDLIKELEERTFSDDSKE
                     NLNVIHEFQRRWFEIGRVPIEKKDEIQAEWQKSIDQTLDKLKISRFESENTRYKERME
                     SVSKMSDGRDKMWSEVKKLRFNISKLEQDVQLWENNLGFFANSKNAEVLLSEFRDKIE
                     RAKTDIKVMKEKEKYLQNLMNDGKK"
     gene            17147..17219
                     /locus_tag="PKY63_07215"
     tRNA            17147..17219
                     /locus_tag="PKY63_07215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17180..17182,aa:Gln,seq:ctg)
     gene            17395..17628
                     /locus_tag="PKY63_07220"
     CDS             17395..17628
                     /locus_tag="PKY63_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00438.1"
                     /translation="MKSLMLSAAFAAMFFLVSCGDGKKEEKKTDSTEVKTEVVDTTLT
                     ETTPTVDVSETNTEAKPVNEPETNTGTPVRGNK"
     gene            17774..20566
                     /locus_tag="PKY63_07225"
     CDS             17774..20566
                     /locus_tag="PKY63_07225"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00439.1"
                     /translation="MKKIILVLLLSSAFASFAQNYITEASGIQIGKVNSFLKLSNNSL
                     LIGSDNGLFSYSQGSANAVLPASIHGVSDMAVDNSGFVWLATHNAGLVKLNSSATSIL
                     QVWNSVNSALPFALKHIEIDPANGVWIASDHAIWLMNGAQFTGFSFPTGTTLSGVKDI
                     LNYGGQTLFLMKDSICRFNGSGFSGINYDEYASLSVTGSGEIFTCNDSLIYKYNGTAF
                     ICDSALSAGCNANVFHLSKLEFFDVLKTGSEYYVFSPSYVMIAANQAPAQLYFPSSGF
                     FMPGMQWHAVFSDFPQLFIGFSDGFLITNFQYFATLSNGYQYLDLYRMRAPVLANGTL
                     FNFNSILGRNVFEAPKGTGKSPMFIAMPWMAGLDQNNQLHLAAQRYTQIGHDFFPGTL
                     KVNSANPSGNTDHARVWKVSRQEIDYHAAHWNQSGYAMPEAIMSWPGNGNALYDEAPI
                     LAPFADINQNGYYEPDQGEYPVIRGEQAIYFIYNDAAAPHTESGGEPLGLEVHGMAYV
                     MRDSSSMTGVNNTDNTIFLHYTIINRSQNDYHQMKLAMFSDIDIGYAYNDYVGCDTIL
                     DSYFIYNAPGVDSVSSGCYYCYSDVLPAMGLTFLSHKMQSFSAYNNIGTLPSYCVDPE
                     ISTHYWNVMNGLFRDGSPMTYGGNGYGGSIPVNYMFPGDPIDSTQWSDMSAGNLPFDR
                     RGVGVIEFDSLGAGQSVCLDFAFVFDDELNGTDNTINVTLLKKRIGEIRTFFFNQQLE
                     CLYYEHNYMQTQAGSYVDTLLAFVDTCVIDPYSPVDSAWINDLTTVGNTVYVDWIVTQ
                     HGDTFYFNNVPYVLQNSGTQLFVLVLRCMFDGRGSQTHLVYLNASFEASPEPGKPILE
                     IALFPNPATDYVTVVFPEGQLPEVLQIINMNGQIIDSYEVSSSSVSIPVAWLASGLYL
                     MHAVRSNESLPFFIQQ"
     gene            complement(20580..21113)
                     /locus_tag="PKY63_07230"
     CDS             complement(20580..21113)
                     /locus_tag="PKY63_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00440.1"
                     /translation="MKSIIWFILFSILASFFASGQNSEYVVKLVKKSNPDKVRYIEEG
                     RRIKIYTSDNTEYTGQLYIPSDSTLRVDAITFAADQIPKIRGNSTGLLVTKIAGGVLG
                     ATGLVVFIMGSIILIDGLNPDVWSEIFLIPFGMIIAGTGALATIAGGSVLFINGKKYD
                     LRDQWDMSIIPVSEMPQ"
     gene            21273..21731
                     /gene="msrA"
                     /locus_tag="PKY63_07235"
     CDS             21273..21731
                     /gene="msrA"
                     /locus_tag="PKY63_07235"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPF00441.1"
                     /translation="MKTIILAAGCFWSVQYKLSNVEGVLSTKAVYANGIVAEATYQQV
                     CSGTTGHAEGVLVTYNDSILTTEVLLRFFFLTHDATQLNRQGPDIGEQYRSGIFYFDE
                     EQRETALKLIEELQQLPKYAGKKITTIVEPASLYNAAEEYHQEYYRKKGF"
     gene            complement(21899..22345)
                     /locus_tag="PKY63_07240"
     CDS             complement(21899..22345)
                     /locus_tag="PKY63_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPF00442.1"
                     /translation="MKEKKYEIGNVVVRYAEESQMRYQLEERLVSLSVSVIEFCKDFP
                     GGKVGDYFTDQLTRATVSAALNYGEAQAAESRKDFVHKLKVALKELREALVCLKIINR
                     SSSFSKVKNGNKIFQETDELVSIFVASVKTSQARLNAANNKISKQK"
BASE COUNT         6264 a         4401 c         5105 g         6747 t
ORIGIN      
        1 cagaccatct gcaacgatga atcgaccacc ctcgatgccg gagctggctt tacgagctat
       61 tcctggacag gtggagctac aactcagaca ctcacagtgg atggcgctgt acttggcgtt
      121 ggcaaccata caattactgt aacggttaca aattcataca cctgttcagc cagcaacgat
      181 atcattatca ctgtcgaaga ttgctcagga atagaaacag ctacccttca gattggcaat
      241 gtgtatccga accctgccag cgaaagcatc agcattgcat ttgctgcaga acacaacaat
      301 cttgtgatca atctttttga tgcaacaggt cgccttgtaa gttcacagtc tttgaaaaac
      361 actacatccg gaaatgtagt agtgatccct gtatcacaac tgtcagccgg agtttattca
      421 ttgcagattg tttctgagga aggaattcag ataagcatag ttgttataga ataaaacaaa
      481 tattctttca aaaaaatccg ggctgaaaag cccggatttt ttttggatta aaaggttcat
      541 aacaaggcaa ggcaattcat acaaattcat taggtttgca aattaaatat gcttatgaac
      601 agtatttttc ttcaggagtt ggatccgcaa atcgacacat gggatgttat actcaggttg
      661 ttcctcagtt ttattgcagg tctcatcatc gggattgagc gcgggcggca taagcaggtg
      721 ctcagtttgc gtccgcatat tctcattgcg gttggttcga cattggtcat gttgatttct
      781 atttatatgc cgcaggtttt tggtgcggat aaaaactatg atccggggcg cattgcggca
      841 caggtggttt ctggcattgg ttttttgggt gccggagcca ttatccggat gggagtcaat
      901 gtaaaaggtc tgacaactgc aacaaccatt tgggtagctt ctgccattgg tctggctatt
      961 ggtgcaggtt tttatctggc tgcacttttt gccgtagtgg cgattttact tgttttgatt
     1021 tttattgatc atcttgaaaa aaatgttttc agggaaagtc tttataaagt ggtaacagtg
     1081 aagtttgatg gcgttgaaga ccgtaaggaa gatgttgtgg ctgtcttgaa aaagcatcag
     1141 gtgcgtattc tgactattaa tacagaaaag gattttgata agcaacaatc cagactgtat
     1201 tttcaggtgc atattgccat gcatgccgat actgaaaaaa tctgcaatca gatttctgat
     1261 atgtttggtc cgggacactt gaaatttgtt gggtttgaaa caatggaata aaaggtatta
     1321 tggattctgt cagagaaatt tatcgtattg gatatgggcc gtcaagcagt cacaccatgg
     1381 ggccgtcgcg tgctgccgaa ttatttttaa agtctgctcc ccaagccaaa aagtatcggg
     1441 tgcatttata tggaagtctt gccttgacag ggaagggaca cctgacagat gttgctataa
     1501 caggtatttt atatccatcc gaagttcagt ttatatggca tacggatgaa gttttgcctg
     1561 agcaccccaa tggaatgata ttcgaagcat tggattcaga tggtaaaata acgaatgagc
     1621 tgaaaatatt cagtacaggg ggaggacaga tttcgcagca tccgggaaaa gagatcagca
     1681 ggcatttgta tccattgcac accatgcatg atattatgaa atggtgcgat gacaatggaa
     1741 cccagctttg ggaatatgtt gtgcaatgcg aaggggaatc cattctttca tatcttgccg
     1801 aagtttggca ggtgatgaaa agcagcgttc agagcggaat tgaacacgag ggtgttttgc
     1861 ccgggccatt gaaactggcc cgtaaagcgg cgagttatca tgcaaaatcg caaggattta
     1921 atcctgccgt catgcgtcgc ggaatggttt acacctatgc attggcagtg gctgaagaaa
     1981 atgccggggg tgggagagtg gttacagcac cgacctgtgg ctcctgtggc attgttccgg
     2041 cagtacttta tttattgcat aattacaatg atgccaccga acagcgcatc atcagggcgc
     2101 tggccacagc cggactggtt ggtaatctcg tgaaacacaa tgccagtata agcggagcta
     2161 tggtgggttg tcagggcgaa gtcggaactg cctgcgccat ggccgctgct gctgcaacac
     2221 agatttttgg cgggacaatc accgaagtgg aatatgctgc cgaaatgggt atggaacatc
     2281 atcttggctt gacctgtgat cccgttatgg gtctggtaca gattccatgt attgaacgta
     2341 atgcttttgg tgcagcgcgt gcactggatg catcgacctt tgctatttta tccgatggac
     2401 gacatcgtat ttcgttcgat gaaattgtgc agacgatgaa acaaactggt cttgacatga
     2461 atgccgatta tcgcgagacc tcgaccggcg gtcttgcaaa agtgatgaaa aacagaaaga
     2521 aaaaatgaga aagataattt tcgctgcatt tttttttgca gtcagtacac tttccattgc
     2581 gcagactgta gtgcgtattc caatcggaaa atcatcggaa cctgctgatg caccggtgaa
     2641 gcttactttg attgcccgca tacagaatta caacgaacat gctgcctccg acagcgattt
     2701 tttaaataag catatcgaca gtccaaaatc tgtcaatatc cttgattcgg gaaacaagtt
     2761 ttacattcat agtctggagg gttgcgaaac gcttgtgttt agtctcgatt cattaaagat
     2821 gctaaaagtg attccgcata ctttttccca aaaagacact gctttgtttc atgaaaacac
     2881 ggtgcttggt tattcatttt catatcatgg cgttgtaaat agttttagtg gaaaacctgt
     2941 tgaatcgtgt tttacacaca ataaaaaata tctgtgggtt acctattatc gcaggtcgtt
     3001 cgacctgaat gccatgcagc catcggctgt agcaattatt gatacagaat gcgactctat
     3061 tgtcagagtc atgccgacgg ggccgcttcc gaaaatggtg gcagtatcgc ccgatggaaa
     3121 atatctggca gtaacgcatt ggggcgataa taccattggt atcattgacg tttcttcgtc
     3181 aaacccgaat gattttcatt atgtacatca ttttgtggtg gattatcgct tatcgttgaa
     3241 gggggatggc aacaaaccca ttaatcgtga ttctgaatgt gggttttgtt tgcgtggaac
     3301 cgtgttctcg cccgacggta aatacctgct tgttggccgt atgggtggag gaggaattgc
     3361 ttttttcgac atggataaaa tgatttatat gggcacattg tggggaatga aaaccaatgt
     3421 gcgtcacctt actattgccg gaaacgattt attggtatcc tgcaccagac ccggatttgt
     3481 tcagaaggcg cccatagatt cactcattgc ctgtatggaa cgcaaacaga acaactatag
     3541 ttcatgggaa aatgcatttg ttggcatcgg tgtccgcacg attgatgtat cgcctgatag
     3601 gaaatatatt ttctcggcca tcaacaatgg aagtaaaatc gccataacag attatacttc
     3661 catggaaatt atctccacgg tcagcgccga ttcatttccg gttgggcttg ctgttacaga
     3721 agatgggaca aaactgattg ttacttcgca aggaagaaac gggattggcg gaggaaattc
     3781 tgtaatgatt ttttcagtag aatactaacg ccagcttggc gaagtgcgct tgttccggat
     3841 gatgtaattc cggttgatgg gcatggtgtt tcgcttcagt cgtttttggt aatgactttt
     3901 atagccaagt gcggagtttt tccgttttac atagcgtttg tagttcttga actgacttga
     3961 gctgcaagaa ctcattgcca tcgttgctac aaaaacgatg agcagaagca tatatttact
     4021 gaagaatctt ttcattatcc atgaaaattt gcgtaaaact atactttttt ttttacatgt
     4081 gcaaatcaaa catatttttt tacgcaaaaa tgctcattta gatacagtga aacgtgaaat
     4141 tttatgaaaa attccagaat cgacgttcta tataattgat aaacagtgat ataaaatttt
     4201 aaaattagtt ttcaacagct gttgatttct tatataaatt tagttttcct gtctcatatg
     4261 ttatatttcc tgacttctga cattattgaa atttggagcg gtattaaaaa tacaactatc
     4321 agatggcaaa ctggttaaaa caaccaattt gaatattttg ccgcactgac tataaccttt
     4381 caaagttaca aatacaaaaa aaacaaaacg gattggttgt atttaggatg tcgcataaca
     4441 ttttgcggat cagaaatcga ggaagagaag ggcgggcgcc gagggcatga tgcctgaaat
     4501 ctgtctgagc tgcataattt aatcagacat tccttgcagc gagttatttc aggctgtggg
     4561 gggcagttca tcgatttccc gcaaaatgtt ccagcgacga gattttcttt tgatactttt
     4621 cttttggcgg gcaaaagaaa agtatgctca gcctgcaagg ctgcactgaa tcagctgaaa
     4681 agccagtgct tacggcaacg aatttggtaa ctgcactaaa tgtcagaagt tggggaaatt
     4741 agttttcaac agctgttgat ttctttgcat tttttcttcc ccgacgtttc gtaattttta
     4801 ttttactttg cagcgagttt ggttcatcaa agaaaaactc tttgatgcta agagggaacc
     4861 gggtgaaaat cccggacagt acccgctgct gtaagtcctg aaaaagggcg cggtaattcc
     4921 cctgccactg ttccgatacg caaggaacgg gaaggcaccg cgccggggac aagtcagaag
     4981 acctgccagg cgttttttca tgaatctttc gggcgtaagg gtcatggagc ttgtttaatt
     5041 ttttaatgtt ttttacatta tgaaacattt tttccacagt tcgctgttat ggcttgcttt
     5101 agccatgcct ttcatgtcaa atgcccaatt ctcaatggat gacatctcat tctgggttgg
     5161 ctctggatcc aaaaaaactg ttcttgtcat tgattttaac gatggcgcca atccgcaatg
     5221 ctatgcctgg ggttatcgtt tcgatggcac atcggttacc gctgaaaaaa tgattgccga
     5281 tattgattcg gccgaccatg cattgagtat ggtggttatg tcaggtttcg taaatgatat
     5341 aacttattta tctcacgccg gcttaggtgg aaatccggac tatttctcca ctttttcagg
     5401 cgatggcgat acgtccaatt gggttatgaa tatgggcggc agcgaactgc tgaccgattc
     5461 tatgtggttt ggcatgtcat acacgccatg ggattcgtct ttcaacccgc tctataagcc
     5521 aggtttgccg gttgctgcca gtccggtaaa tgggtttgcc gacatccgga ttttcaatac
     5581 cattatttat cccaatccgg ttcgtgattt ttttaccatt aataattacg ggaactttga
     5641 ccgaatgcag attttcgatg tcaacggcaa acttgtttgt gaatacggaa ttttggaggc
     5701 caatccggaa tatagtctga atgcggttcc caagggtttt tattacgcac tgttttacaa
     5761 taacaacgct gttcttcacg gcgaacttat tatcaaggaa tgaaaacaat cgttttttta
     5821 tttctgatac aactttgttt tttcgcaaaa gcgcagtatc cgcctgccgc agggcaggcg
     5881 ggctctactg ccgttccgca cgacagcgcc tgctttattg attgggctgg cagttgcagc
     5941 attgttcgcg gctttgtgaa tgtttcagat acaacagttg ttgccgggtc ttccaactat
     6001 gcctcttatg gcgctgaatc caatgttgcc ggcattgccg acaacgtggt tgtgagtctt
     6061 ggcgacggtg gtgtggctga tatttttttc aatgttccgc tggccgatgg ttccggattt
     6121 gattttgccg tatttgaaaa cgcgctcaca gacttgtttc tggaacttgc atttgtggaa
     6181 gtcagctccg atggaattca ttttttccgc attccttcgt attcgttgac cgacacaata
     6241 gtgcaaaccg gtggtttcgg aaacaccgat ccgacaaaga ttcacaacct ggcgggcaaa
     6301 tacagagcgc ttttcggaac ccctttcgat attgcggatg ctcccgatac tgctttactt
     6361 cgtaaagatt caatcaccgt ggtgcgtttg attgatgttg ttgggagtat tgaccctgta
     6421 tatgcttcgc gcgactgcga gggcagaaag atcaatgacc cgtttccgac tccttttgaa
     6481 tcatgcggtt ttgatctgga tgcggttggt gttatcaaca accgcagcaa tactggtttt
     6541 gacgagtatc agaaaatagt tcatgtttat cctgatccgg caagcgacgt gatttatttc
     6601 aattcatctg agactgagcg cgttttcatt tattcgtgca gcgggcatct gatgcgggaa
     6661 tacaatggtt cggaaaaatc ggctgatgtt tcaggattgg ataatggcat ttattttctt
     6721 agaacttctg aaggaaaatc atcaaaattc atagtatttc attaatgaaa gggttgtttt
     6781 tcatattgtt tcttttgtta ggtttcctgt tgaatgctca gcagcaaaat gatacaatat
     6841 ggctgaaacc ggttgacatt tcggccccgg ccgatgattc ggtattcatg atttccgttt
     6901 ccggcaagct gattcctgcg gatgaaaatc tctccattga aaatgctttg tcgcgctatt
     6961 cacctgtgtt tttcaaaact tattctcccg gtggtattgc aagcatttcg catcggggct
     7021 tcggggcttc gcagactttg tttgtgtgga atgggtttgc tttgaatcag ctcacgcttg
     7081 gacagccggc gttgaatggc atgcaggtga atgaaaacat gcattttgga atcgtgtccg
     7141 gaacattggc tgcgtctgat ttttccggag gtctgtcatc gtatatctat gtagatgatg
     7201 gcttttatcg cgatagcagt cttatgcgaa gacttgtcat ttcaggagga agttttggag
     7261 ccggctctgc ttctttcttt tcagactaca gaataaaaaa gtcacgtgtt tctttcgaac
     7321 tgtcaggaat gaatgtggtt aataattaca ggtttcataa caatgcagaa ggagcaggtg
     7381 ttgagcaatg gcctcttcaa aaccgcgtta acgcttcata ttttggcatg actgttaaaa
     7441 gcaatctcct gattccgttg aaaaatggcg acttcaaatt ttccttatgg gcgggttcac
     7501 aatttaatga atcgcctgca cagttgctga gcccgcagct ttctgacaat gaaagtcagg
     7561 aaaacagatt tgtcagaatg agtggatttg tccctttact taccgctgct gatgcagggt
     7621 tacagattcg cttctttttt agtgccgata cttttttata ccgaaacaaa cagttgtcca
     7681 tcagtgatgg aacagcatca caaacgctgg cgacacgctg gaatgcctat ctgcgtgcag
     7741 gtgccgggca tgaattcaag tttgagtatt atcctgaatt caacagggtg ctttcagaaa
     7801 atttttcaaa acccgttctc agtacagatc agcgtttgaa tctgaattat tgtttaaaca
     7861 gatccgatgt tgtaaaagcg attatctgga cacattttat tactcgcaat ttcgatgaat
     7921 tgttcccgtt gccgggactc tcattgagtt ttatgccagg caacagttca aaagcgaatt
     7981 ggaaaagatt gaagtggctg agtgtgaatg caggaattgc gcgcaatatg cgcatgccga
     8041 ctatgaacga tttattctgg gaacctggtg gaaaccctta tctgaagccc gaagacgcat
     8101 ggatggcgga tatggctatt gttgcatctt ctgcttttaa aaaaatcaaa tgggatttta
     8161 aagtaatgcc ttttttctct gaaactttta atctgattcg ttggactcct gattccttat
     8221 cgtctcaatg gattgcttca aatgtttctg aatcgcgtca atatggcgta gaaggcgttg
     8281 ctgcgtgttc ttattcatgg cagcaaaata caattaccgg atcgatgaat gtgacccgtg
     8341 tttttgctca ggatatgagc ggcgataaag tattcactct gatgtatgtg ccagataaaa
     8401 cgatgaattc atctgtgtct tttgaacacg gcatctggag cggaggatac gagtttcatt
     8461 ataccggaaa acgctattcc agcagcgata atgaccgata catgcccgaa atatttttgc
     8521 atgatgtgtt tttttctgtt gtgaaaaaaa taaaatcaga tcagttgtcg ctctctttgc
     8581 gctttaataa tataatgaat gccgattatc agtacatcgc ctggtatccc atgccgcgaa
     8641 gaaattttca tgtcacgctc aaatggaaat ggaatgaata ggtggctgct tatattattg
     8701 cttgctttgt cgctgtattc atgcaccaat attgaattcg gcgaagtgaa cacattggac
     8761 aattcgccct cttcttttca taatggtgcg tttgttatca acgaaggact ttttaattct
     8821 ggcggaggcg atgtatcgtg gattgatttc agcaatggca ccatcagcaa tgaaattttc
     8881 cgttcagtga atggctttcc acccggagac atcccatttc atgcggcttt gagcggtgac
     8941 aatattttcc ttactatcaa caactccgca aaagtgtata taatcaacga tgatttcgaa
     9001 gtggttaaaa taattgaagg aattgcatca cctcgcgaaa tctgcgctgg tagcggaaat
     9061 acaatgtttg tgtcttcatt gtacaagccc tacatttatg tgattgatgc ggagcaggga
     9121 attctgagcg acagtattta cactgaacga ccggtcgaaa atctgttgct gcatgccggt
     9181 aaactgtggg ctacacactg gtcgaagctg gtatcaacac aaaccaataa cgctttgctt
     9241 gtcatcgaca caacatccat gcagcttgaa gactctgtca tcattggtgt agagcctaac
     9301 agcatgggtg ttgatgtgaa tggtttcatc tgggtattgt gcagcggtgg atttgatcaa
     9361 gcagaaatgg caaagattgt tgtcattgat ccgctgtcgc attcaattat ccgaaacatt
     9421 ccatttgcat cttcgtccga ttatccttca gcattggtga tgaatgctgc gcgtgattca
     9481 ttgtactttg ttaatcagca catctataaa atggctgtca gcgctacttc ggtgcctaca
     9541 actgcatgga tacaatcttc aggaaatact ttttaccgcc tcgctgtgaa tccaacggac
     9601 aactcgcttt gggtgagcga tgccggcgat tatgtgcatg caggccaggt gctgcattat
     9661 tctttatcag gcaatttatt gcagactttt aatgccggcg tggtgccggg ctatgttgtt
     9721 tttcgggagt agggtcgctt cgcgatgttt aaacttcgcg cagcttgtag ctgtattcac
     9781 tgacctgcag cgcaggtcaa agaataatag cccggcctgc tgtgtcacgt tttacgtgaa
     9841 acggccggga aaaagatgaa gcaaaaccga cctcagagag gtcgaataaa gacgatatta
     9901 acgcaatgat ttttttgaaa ccaggggcat tagaccctgt cagggtcttg tttttgtgct
     9961 tatttacacc cggcttcgcc ggatgctatt attgtttgac atcctccgga tgtcagtatt
    10021 attttcgaga aatgatacaa tgtcttaatt gtcgggacac accaaaaggg attataattt
    10081 tgtatgaatc actatatctg atttttgcaa aaagttaaaa ggcaagtgtc gatgccagga
    10141 atttagactc cgcgcaactt gcagccaaaa cgattttcaa aatgaataaa aagtcaacct
    10201 attcgattac aaaataatta ataatgtcac ccgataagct gtcaaaagca aatattatgg
    10261 aaggaaatta cctgacatat ttaaataatt agtaaggaaa ttacctgacg aatatttaag
    10321 tcgggttatc ttgtatgttg tttttcggga gtgaaggtgc tccgtgaagt ttaaacttcg
    10381 cgaagcggat agctgcaaaa atccgcaata gcacagcttc cacacaaagg tttgcgtgcc
    10441 ttgcagacat agcgtccatg caaaatgagc cagtggtgca ggtcgtggag cacatcagcg
    10501 ggagcgatgg ataccagcgc cttttcgact gccagcgggg ttgtggctcc gggagccagt
    10561 cccagacgta cgcttactcg ttgcacatgt gtgtcaaccg caataacatt ctgttggaac
    10621 aaaacggagg caagtacatg tgctgttttg cgccccacac ccggcaattg ctgcatctct
    10681 tcgggatcgg tcggtaattt ccccttgtat tttccggtca gcatttcggc catggctttt
    10741 aggttgcggg ccttgttgtt gggataggaa caggatctta ttttttcata aatcgcagat
    10801 tcagtagccg cagccaatgt tttggcgtta gggaatgttt taaaaaaatc aggtgtaatt
    10861 ttatttacgc gcacatcggt gcattgggca ctcagaatca cagctacaat cagctcatac
    10921 gggtttttat aatcaagctc ggttttggga atcgggttgt tctgctgaaa tatttcaatg
    10981 atttttgcga gtcgctgttt gcgcgtcata acgaataact atttgatatg ataatttgac
    11041 atgtcgctca gtttatgcga tttcaggtac tcaatcagct cggctgtaac tttgtgaaaa
    11101 atgccaccca tcaggcattt gtcaaatggg cagatgggct tatcgaacgg acaggtttta
    11161 atggatattt tcccttcaat ggcttcataa atatcaagca tggaaatgtc tgatggctcc
    11221 atggcaagtg aaaacccgcc agcagggccg cgaaccgaac taacgaaacc cgatttaacc
    11281 aatgtttgca ttaccttggc tatatggttc ttactggcac cggttttttc tgcgattaca
    11341 ttaacattca taaaattatg ttcgctattc gaaatcaata ccaaggcatg aatacctatc
    11401 gaagctgctt cgctcaattg aattggtttg ggcattattt cggtatttag taaaattcgg
    11461 tacaaaaata cttaaataat ttttattctc tgacaaagac acatttttat tcagggtcaa
    11521 cgcatttgaa tgcgtctgtc ctgcaatatt attgtttttg ataccggcga tttttgggaa
    11581 accactatat ttgtaaaaaa atcagaaaaa atgaagaatg taaaagacat gggatttaac
    11641 acacagcttg ttcatgcagg tgattttgaa gatgctttcg gaagtgctgt aatccctatt
    11701 tatcagacat cgacctttgc ttttcgtaat gcgcagcagg gtgccgaccg ttttgcggga
    11761 aaagaaaaag gttttattta tagccgcatt gggaatccga ccattggtgc tcttgaagac
    11821 aaactggctg tgatggaaaa cggttttgga ggtattgcaa caagctcggg catgggtgct
    11881 gtatctactg tgtatcttgc catgttgtcg caaggcgacc atatgatcag ttcctctgca
    11941 gtgtatgggc caagccgtaa tattatggaa aatcactttg tgcgttttgg aattgaaagc
    12001 acttatatcg ataccagtga tttgagtctt attgaaaagt caatcaagcc aaacacaaaa
    12061 ttgttatata ttgagtcacc ggccaatccg accatgacaa taaccgacat caaagccgca
    12121 tcggatctgg ctcacaagca tggtatcatg gtttgtgttg ataacacttt cagcagcccg
    12181 gcacttcaga atccattaaa ccttggagct gatattgttc tccattcact tacaaaatac
    12241 attaatggac atgctgacat tgtcggtgga gccgttattg caaaaaccga agaaacctat
    12301 aaaaaactcc gggccattat ggttggattt ggttacaata tggaccctca tcaggcctat
    12361 atggttattc gtggagcaaa aacactcgca ttgcgtgttg agcgcgctca gcaaagcgcc
    12421 atgaaagtgg ccgaatatct cgaaagtcat ccgaaaattg cctgggttaa atatccgggc
    12481 ctgaagtcgc atccacagca tgaactggcg aaaaaacagt cgaaaggttt tggcagcatg
    12541 atcagctttg agatgaaagg aggatacgag gcaggtgtaa aactcatgaa caatgttcat
    12601 ctgattatgc ttgcggtttc gttgggtgga gttgaatcgc ttattcagca tcccgcttcg
    12661 atgacacatg ccggtgtttc ttctgaaggc aaaaaagccg cttttattac cgatggtctt
    12721 gttcgcttgt cggttggaat tgaagaagta gaagacattc tgaatgatct cgggcatgca
    12781 ttggaaatgg tataaatgaa aaaactgatc atcatagggc tactgttcta ttgcactggc
    12841 tttgtcaatg cacagaaagt agccgtggtg ctcagtggtg gtggctcgaa aggagcagct
    12901 catatcggag ttctacgagc actcgaagat aatcagattc ccatcgatta tatagcagga
    12961 acgtctattg gagccattat cggtggacta tacgccagcg gatggactac cgatcagatg
    13021 gaacaattga ttacatcgga gcaatttttg atgtgggcta cgggtgccat tgatgaaaaa
    13081 tatgtgtatt atttcaaaca gcccgaaccc gatgcatcct gggcttcgtt tcgtgtaaat
    13141 gccgatagca tctggagttt cgatgtaccg accaatcttg tttcatccgg gcagatggat
    13201 ttcattttta tggaaatttt tgctcaggcc aacgctgttt gtaaaggtga tttcgattct
    13261 ctctttgtgc cttttcgttg tattggagcc aatattacag attcaaaagc tgaggttttc
    13321 agtggcggaa atttggtgaa atccattcgg gcatcgatga cctatccttt gtattttaaa
    13381 cccattacca ttgatggtaa aatgtatatg gatggaggca tgttcaataa cttcccggcc
    13441 gatgtagcat taaaggattt cgatcccgat attattatcg gatccaaggt ggctgccgaa
    13501 aatgcggtac ccgatcccga caatatcatg tcgcagcttg aaaacatttt tatgatcgaa
    13561 acatcctacg atttgccctg caacagcagt gtgctgattg aaccccaaat accgacaacc
    13621 aatgttgttg atttttctca ttccgccgag tatatcaaat tgggttatga tgcggccatg
    13681 tcaaaaattc cgcaaatcag ggaatttgtt atagacagtg tttccaagga cgaaatgcaa
    13741 ttgcgccgcg atacttttaa taaacgcaag ccacctttgg tttttgatga ggttgaggtt
    13801 acaggcatga ataaaatgca gcgtcagtat gtgcttaatt atttttcgcg ccgcgagacc
    13861 gttggaattg aacgaacgcg tgaagactat taccgcttga tgacggatca tgctatccgc
    13921 actatttatc ctatggctga ttacaaggat acctccggga gttttaccct gaatctggaa
    13981 gtgaaaaaag aatcgaatat tttactgcgt tttggaggac ttgtttcttc caacccggtc
    14041 aatcaggcct ttgccgaagt gagttataaa tatcttcagc gtaatgctgc tactgtcagg
    14101 gccaatatgt atgccggccg tttctacagt tcggcattac ttggaatgcg gctcgatttt
    14161 cccggacatc cgcaatatgc cttgtttggc cggattgttt acaatcagtg ggattatttc
    14221 aaaaccactt cgcatttttt tgaagataaa aatccaagct atctgattgt taacgattat
    14281 cattgggatt gcggtgttgt tgtacctgcc cgaaacaaag gaaaagtaac actggctggc
    14341 tctgctatga ttattgatga tgaatatttc caggataatt atttcacacg aaccgacatc
    14401 cctgacaaaa caaatttcaa agcatggagc gtttcagccg aatgggagcg gaatacgctc
    14461 aataaaaaac agtatgccaa caagggaact ctgcttcatt tgcgtaccgg ttttgttcag
    14521 ggtaaggaaa atacagtgcc tggtaccacc tcggtgcttt ctgtcgatta ttcggctcat
    14581 catagctggt tttacatgca gtttatctat gaaaattatt tcagctttat ggggcgttta
    14641 cgcatgggtt ggtataatga catttatttc tcaaatcgtg atttttttgg tagttatact
    14701 gcaaccatgc tttttgccaa tccatttcag ccattgcctg aaatgaaagc actttttatg
    14761 cctcaatacc gggctcatca gtatgccgga gccggattga aaagtatttt tatgatccgc
    14821 gataattttg atatccgtgg cgaggcctat ctgtgtcagc ccattcgcga tataaccatg
    14881 ggaccgagcg gcatttatgc tacatacggg ccatggctca gaagccgtta ttttgtggct
    14941 tcggccggac ttgtttttca tagcccggta ggaccgattg ccattacagc cagttattac
    15001 gataaatacg atgagccatt gatgctgtcg ttttattttg gatatattct atttaatccg
    15061 aaagctgtta attgaaaagg tctttaattc attaagtttt ttaaatttgc agtccttatt
    15121 gtgaatattg aatttcaaag tgatggaaga aaagaaagaa accccggctg aaaatgctga
    15181 ttctaagctg aatgaaagcg aagtcgttca acaggaagtt aatgaatcta ctgaggttga
    15241 aaccaacacc ccggtagcgg aaaatgaatt cgcccatggc gaagattcgt atgtgcttga
    15301 agaagcggat gaaagtccgc aggaagaaga gcacattact gaaaatcatg atttggatta
    15361 cagcaaatgg gacaactata acctggatga aattaaggcc gaagcgctca atgtcattca
    15421 acagatcgaa gatttgatgc aggttggtca ggccttgaaa aaactccgcg aattacataa
    15481 tgctcaaatt gaaattgagc atcagaaact tgcggaactg ggcgaagatg accccgaagc
    15541 acatgaacat aaaactgaac ttgataaaaa aaccggtgaa ttcaatgcca tctggggtat
    15601 ttatcaggag aaaagaaaag aacattttga taatttagtc aggcagcagg aagaaaattt
    15661 cgagaaaaaa cttgtgctgc tcgatgatct taagaaactt attgaagaag gtgagccact
    15721 gaaaaacact tttgatgagt ttaaacgtat tcaggaaaaa tggcgcgaaa taggcatggt
    15781 gcccaaggat aagagcaatg acctatggct caattacaac catcatgttc agatgttttt
    15841 agacaaagtc aagctcaatc gcgaattgcg tgatcttgac atgaaaaaga acatggaaat
    15901 taaagttgaa ttgtgcgaaa aagctgaaga actgatattt gaaaagtcgc tgcatgaatc
    15961 atttaaaaaa ctgcaggatc tgcatcggca gtggaaggaa accggccctg tgccatcgga
    16021 tgtgaaagat cagatatggg aaagatttcg caatgcttcg gaaaaaatca gagaaatacg
    16081 tatttcgcat tacgaagaac tcaataaaaa actcgaagcc aaccttgaag ccaaacaagc
    16141 gcttgttgac aaagcaagaa atattgcttc ggccgaaatc aatacgatga aggaatggaa
    16201 tgctgccaca aaggaaatga atgagctttt tggcttgtgg cgaaccattg gaccggctcc
    16261 gaaagaaaac aatgataaag tctggaccga ttttaaagga attcttgatg tattttacgc
    16321 tgcacgtaaa gagtatttca atgccattcg cagcgaactg aatgagaact ataataaaaa
    16381 actgaatatt tgcctcgagg cagaagcact cagagaaagc aatgactgga agaaaaccag
    16441 caatgaatta atccggctgc agaatgagtg gaaaaaagtt ggccctgtgc caaaagtaaa
    16501 atcagatgta atctggaaaa gattccgcgc tgcctgcgat gagtttttca atcgcaaaaa
    16561 aaactatttc gaaaacctgt caacggttga agctgaaaac ctgcagaaaa agatggatct
    16621 gattaaagaa ctcgaagagc gtactttttc tgacgacagc aaagaaaatc tcaatgttat
    16681 tcatgaattt cagcgtcgtt ggtttgaaat tggccgggta ccgattgaga aaaaagatga
    16741 aattcaggcc gaatggcaaa agtccattga tcaaacactt gataaactta aaatcagtcg
    16801 ctttgaatca gaaaacaccc gctacaaaga gcgcatggag tctgtgtcga agatgagtga
    16861 tggccgcgat aaaatgtggt ctgaagttaa aaaactgcgc tttaatatat ctaaactgga
    16921 gcaggatgtg cagctgtggg aaaacaacct gggctttttt gccaattcca aaaatgccga
    16981 agtcctgctg agtgaattcc gcgataaaat tgagcgagct aaaactgata ttaaggtaat
    17041 gaaagaaaaa gaaaagtatc ttcagaacct gatgaatgat ggtaaaaaat aatttttaat
    17101 ttattgttgt gcgaaaaaaa acttttgtat ttttgcacac cgaaactgtc ctgtggtgta
    17161 actggcaaca cgtctgattc tggatcagaa gagtttaggt tcgaccccta acaggacaac
    17221 aaagtctgaa agccgctgat gaagcggctt tttgcatttt aatccctcga agggttcaac
    17281 accttttgag ggattgcaca attcgagggg gttgcaaaat tttttttaga aaaataaaat
    17341 atcgcagaat tttattgcgt tattcaaata ccaataacct aaaaaccaaa tatcatgaaa
    17401 agtctgatgt taagcgcagc ttttgctgcc atgttcttcc ttgtttcatg tggcgatggc
    17461 aagaaagaag aaaagaaaac cgacagtacg gaagtgaaaa ccgaagtcgt tgacacaacg
    17521 ctgaccgaaa caacgcctac agttgatgtt tcggaaacca acaccgaagc taagccggta
    17581 aatgaacccg aaaccaatac cggaactccg gtgcggggga acaagtaaaa tcgcctgtaa
    17641 ctttcaataa caaaagaggc cgctgcaaag cggcttcttt gcatttttag atgcatgatc
    17701 cgccaaccga aaactgattt attgttgact ttcatcagac ctgcctataa ttttggagga
    17761 caaattttta tttatgaaga aaattatact tgttctgtta ttgtcatccg cttttgcttc
    17821 atttgctcag aactatatta ctgaagcatc aggaatccaa atcgggaaag tgaattcttt
    17881 tctgaaatta tccaacaata gtttattaat tggctctgac aacggcctgt tcagttacag
    17941 ccaaggcagt gctaacgctg ttttgcctgc ctcgatacat ggagtcagcg acatggcagt
    18001 tgacaacagt ggttttgttt ggcttgccac acacaacgct ggtttggtga aactgaacag
    18061 cagcgccact tcaattcttc aggtgtggaa ttcagttaat tcggcgttgc cctttgcatt
    18121 gaagcacata gagattgacc ctgcaaacgg cgtttggatt gcttccgatc atgccatctg
    18181 gctgatgaat ggcgctcagt ttacaggttt ttcttttcct acggggacta ctttatcagg
    18241 agtgaaagat attctgaatt atggtggaca aacgttattc ctgatgaaag attctatttg
    18301 taggtttaat ggatctggtt tttccggcat caattacgat gagtatgcat ctttgtcagt
    18361 aacaggctct ggtgaaatat ttacatgcaa tgatagtttg atttacaaat acaacggaac
    18421 cgcattcatt tgtgattcgg ctttgagtgc aggttgtaat gccaatgtct ttcatttgtc
    18481 aaagcttgag ttttttgatg ttttaaaaac cggtagtgag tattatgttt tttctcccag
    18541 ttatgtgatg attgcagcca atcaggcacc tgctcagtta tattttcctt caagcggctt
    18601 ctttatgccg ggcatgcaat ggcatgcagt cttttccgat tttccgcaat tattcattgg
    18661 cttttcggac ggatttctta ttaccaattt tcagtatttt gcgacactga gcaatggata
    18721 ccagtatctg gatttatacc ggatgcgggc gcctgtttta gcaaacggca ccttgttcaa
    18781 cttcaattca atactgggca gaaatgtatt tgaagcaccg aaaggaaccg gcaaatcgcc
    18841 gatgtttatt gcaatgccat ggatggcggg actggatcag aacaaccagc tgcatctggc
    18901 tgcccagaga tatactcaga ttgggcatga tttctttcct ggcacactga aggtcaacag
    18961 tgccaatcct tcgggaaata ctgaccatgc gcgggtttgg aaagtgagca ggcaggaaat
    19021 cgattatcat gcagctcact ggaatcagag tggctatgcg atgccggaag ccatcatgtc
    19081 atggccgggg aatggaaacg ctttatatga tgaggcgccg atactggctc catttgccga
    19141 cattaatcaa aacggctatt acgaaccgga ccagggcgaa tatcctgtta tcagaggcga
    19201 gcaggccatc tacttcattt acaacgatgc cgcagcccca catactgagt ccggtggtga
    19261 acctttgggt cttgaagtac atggaatggc ttatgtcatg cgtgattcat catccatgac
    19321 aggggttaac aataccgaca acaccatctt tttgcattat acgattatca accggtcgca
    19381 aaatgattat catcagatga agctcgccat gttttccgat attgacatag gatacgctta
    19441 taatgattat gttggttgcg atacaattct cgacagttac tttatttaca atgctccggg
    19501 tgttgattcc gtctcgtcgg gatgctatta ttgttattcc gatgtattgc cggcgatggg
    19561 actgactttt ttaagtcata aaatgcaatc attctctgct tataataaca ttggcacatt
    19621 gccttcttac tgtgttgatc cggaaatatc cacgcattac tggaatgtta tgaacgggtt
    19681 gttcagagat ggttcaccta tgacttatgg tggaaatggc tatggcggat ccatccctgt
    19741 gaattatatg tttccgggtg atcccattga ttcaacgcaa tggtcggata tgtcggccgg
    19801 gaacctgcct tttgaccgtc gtggagtggg agtgattgag tttgattctc ttggtgccgg
    19861 tcaaagtgtg tgtcttgatt tcgcctttgt ttttgatgac gaattgaacg gaacagacaa
    19921 cacaatcaat gtgacattgc tgaaaaaacg gattggcgaa atcagaacat tcttttttaa
    19981 tcagcaactc gaatgtctct attacgaaca taattacatg caaacacagg cggggagtta
    20041 tgttgatact ctgcttgcat ttgtcgatac ttgtgtgatt gatccgtatt caccagtcga
    20101 ttcggcttgg ataaatgatt taaccactgt tggaaacacg gtgtatgtcg attggattgt
    20161 tactcagcat ggtgatacat tttatttcaa taatgtgcct tatgttcttc agaattcagg
    20221 cacgcagttg tttgtccttg tgcttcgctg catgtttgat ggccgtggaa gccagactca
    20281 ccttgtttat ctcaatgcat cgtttgaagc ttctccggag ccgggaaagc ccattctgga
    20341 gattgcattg tttcccaatc ctgccacgga ttacgttacg gttgttttcc ctgaaggtca
    20401 attgccggaa gttcttcaga tcataaacat gaatggtcag attatcgatt cctatgaagt
    20461 atcttcttcg tcggtcagca ttcctgttgc atggcttgcc tccggtcttt atctcatgca
    20521 tgctgttcgc agcaatgaat cattgccttt tttcattcag caatagtgca tagttgaaat
    20581 tattgcggca tttccgaaac tggaataatg ctcatatccc attggtcgcg aagatcgtat
    20641 ttcttgccat ttataaataa cacggagccc cctgcaatcg tagccagcgc tccggtcccg
    20701 gcaattatca ttccgaacgg gatcagaaat atttcggacc agacatcggg attcagccca
    20761 tcgatgagaa tgatgctgcc cataataaaa acgaccagac ccgttgcacc cagtacgccg
    20821 cctgcaattt ttgtgaccag cagtccggtg ctgttgcctc tgatttttgg aatctggtct
    20881 gccgcgaaag tgatagcatc aacacgtagg gtgctgtcgg atggaatata aagttgtccg
    20941 gtatattctg tattgtcgct ggtatatatt tttatccggc gaccttcttc aatgtatcga
    21001 actttgtcgg gattactttt ttttaccagt ttgacaacat attcggaatt ttgtcctgat
    21061 gcaaaaaatg aggccagaat actaaataat ataaaccaaa ttattgattt catagataaa
    21121 gtggttgggt tatgcaaatt aatacaatta ttttattgaa tgcaaatgaa taacatataa
    21181 ttacaatata gatttattga tttatgacaa atagcagttt atagctttaa agacaatgtg
    21241 ttaacccttt gtttgtgatg taattttgta ttatgaaaac aattattctt gcagccggct
    21301 gtttctggtc ggtacaatat aagcttagta atgttgaagg agtgctttca acaaaggcgg
    21361 tatatgccaa tgggatagta gctgaagcaa catatcagca ggtttgttcc ggtaccacgg
    21421 gacatgccga aggtgttctt gtgacgtaca atgattcgat tttgacaact gaagttttat
    21481 tgcgtttttt ctttcttaca cacgacgcca cgcagctcaa ccgtcagggt ccggatatcg
    21541 gcgagcaata tcgttcgggt attttctatt tcgacgaaga gcagcgtgaa actgcattaa
    21601 aacttattga ggagctgcag cagttgccga aatacgccgg caaaaagatt acaaccattg
    21661 ttgaaccggc ctcgttgtat aatgctgcgg aggaatatca tcaggagtat tatcggaaga
    21721 aaggattctg atttaagaac aaggatttga gatttctgat ttttgatcgg attatgattg
    21781 gtaaattacg aaattttgaa tagaagttcg ggaattatga actatgtttt tttcgtgatt
    21841 ctacagtcat tttatttttg attaagacca gagacgtata atttgattga atggcgaatt
    21901 atttttgttt gctgatttta ttgtttgctg cgtttagtct ggcttgcgaa gttttcacac
    21961 tggcaacaaa aattgaaacc aactcatctg tttcctggaa aatcttattg ccatttttta
    22021 cttttgaaaa cgatgatgac cggttaatga ttttaagaca taccaatgct tcacgaagct
    22081 ctttcaaagc cactttaagt ttgtgaacaa aatcttttct ggattcggct gcctgagctt
    22141 caccataatt cagcgcagct gaaactgttg ctcgggttag ttgatcagta aagtaatccc
    22201 caacttttcc acctggaaag tctttgcaaa attcaatcac agaaacagag aggcttacaa
    22261 gcctttcttc caactgatat cgcatctgtg attcctctgc atatctgaca actacatttc
    22321 caatttcata cttcttttct ttcatataaa aaagtttaag gttgaaagtt taaagacctt
    22381 agtgttcaat gaacttgtaa acaaacttcg caatgacaat tctaggaatg ttcaaaaata
    22441 acagttctta caaaaaaatc aaattgatca tccttaataa tgccagtgat cgactcaggt
    22501 agtccaacat tccatag
//
LOCUS       DAOPKE010000045        22492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2440_length_22492_cov_12.258100, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000045 DAOPKE010000000
VERSION     DAOPKE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2440_length_22492_cov_12.258100"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1018)
                     /locus_tag="PKY63_07245"
     CDS             complement(149..1018)
                     /locus_tag="PKY63_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HPF00443.1"
                     /translation="MKFSRELQLPVLPEKETEKNRNIRNAYVGIPETGILIHHGLQFV
                     PDLKKAVLTELFRGRPEISTDITKEEQAMVSALCPTNAFEKEPFCLNLGKCVFCKECQ
                     FALPDKIRFTNDYKLATNDQEMLKIYPGQKEPIRFNDELRRKEVRKLFRKSLKLRQVS
                     AGGDNSCEMELNASGNVNFDMGRYGIEFVASPRHADGMVITGPITQNMAEAIRITWEA
                     IPSPKICILAGVDAVSGGIFANSPALNRKIAEPVTDCKSAPGNNIKVDLYVPGNPCHP
                     LTFINGVLSLIGR"
     gene            complement(1031..2575)
                     /locus_tag="PKY63_07250"
     CDS             complement(1031..2575)
                     /locus_tag="PKY63_07250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPF00444.1"
                     /translation="MKESSILLNQNPAVFRLSDLPMIDYVQFWQEVSAMMKNENNHIL
                     AYFPLPDGADKWRFICAVADDINKNIQVFSHECSREISKLESLTPEINAMHVFEREIH
                     ENHGVDFPGHPWLKPVRWPHNRYDQSSCMNTYPFFSSDSIELHQVGVGPVHAGIIEPG
                     HFRFICNGEKVYHLEIHLGYQHRGVEDLFVSADSNIKRAVLSDSIAGDTAVGHSLCHA
                     QLMEELAGKTPDETLQMERAIALELERIAFHIGDTSALCADIAYQLGQVVCESLRTVV
                     INTTQYWCGNRLGKGFIRAGGTHYPMTKEVANEILKNLQYVENRYSEMAAHLFSMPGA
                     LSRFDGIGIVSKEQGLRAGLTGMAARMCGIPRDVRTTHPFQAYKKYKYKPEIFTSGDV
                     LARALMRRCEISKSIELIRTIVGDILSRIANPRQNDDEKNTAPNYNLSLAADSVCISM
                     TEGWRGEIVHVAVTNEKGEISKYKITDPSLHNWTGLALAVRNQEISDFPVCNKSFNLS
                     YCGYDL"
     gene            complement(2582..4012)
                     /locus_tag="PKY63_07255"
     CDS             complement(2582..4012)
                     /locus_tag="PKY63_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPF00445.1"
                     /translation="MELILFFSLTLFFALAIFFMRSKLFTRGASLLFSVSLIGLTIHG
                     WMHIDESPVHFFTFSHEGLLLLSALALLSLPTFYHGFRYTQNASVKHFNIYHISMMAL
                     MTFMTGAYLSDSMTMLWVFVEATTLSVAALIYHDRTPQSLEATWKYVFICSVGISIAY
                     VGILLLGFIYGRYDAANLTFDEFSLLVSQANPIYLKVAFVFVLVGFSTKMGLFPMHSV
                     TIDAHSVAPAPVSALISTTLMNVGFLAIFRVFTLFSSTEILPFMQHSLVLTGILSLLI
                     AAGYMLKAYHIKRMFAYSSLEMMGLVAIAMGSGKSGYYAAILLILLHSLVKSSIFFHM
                     GQFKNVTHSYTLKQVGNYMKSYPAGGLLLLCCVVFILAIPPSGIFISEILIFKSIIAQ
                     QHWITFVVTALLLTAVIYALLTRVLHILFSDPAEPLTEAEAPGKARPVETIPQFVLLI
                     IVAVLCFYQPDFLNEAINASIANLPK"
     gene            complement(4017..4631)
                     /locus_tag="PKY63_07260"
     CDS             complement(4017..4631)
                     /locus_tag="PKY63_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00446.1"
                     /translation="MIEFLIVAFAATLIYLSAAQRFRVFARFMGVQGVLIFGIAFLEL
                     NEMATGNLILVVSETLVFKGIVIPFMLFYIIRRTQVFKVHAQATPSFYILLLNAISLI
                     VSVIIAYTLQNPFINIVFMTVALYALLSGLIIIVTHKLIISHLIGFLIIENAVFLFTI
                     ALGNEMPALINIAMLLDIFVGVLIMGFFGLRLNPDTGELTQLKD"
     gene            complement(4628..5545)
                     /locus_tag="PKY63_07265"
     CDS             complement(4628..5545)
                     /locus_tag="PKY63_07265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPF00447.1"
                     /translation="MMTIIFILIAALFFPGIILRSKSIFAGRKGPGLFQPIKDIALLF
                     QKGNVISTTTSAVFKIAPAIGLASVLSALLVLPFGPFPSMLSFNGDIVFFAYMLALGK
                     FFLILAALDTGSAFEGMGANREALYSLLTEPAFFILIGTLAMLTGYTSFNEILVGLYN
                     PDNSYILIYSILSVIILAQIMQVENSRLPVDDPKTHLELTMIHEVMILDYSGFDKALI
                     HITTWLKFALYSTLIFDVLIPIQWNIEIQLALFALVIFFMAVYIGFIESFKARHKLGR
                     NPAFLFTISSMALLAFVVVLILTEKMILS"
     gene            complement(5548..7539)
                     /locus_tag="PKY63_07270"
     CDS             complement(5548..7539)
                     /locus_tag="PKY63_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPF00448.1"
                     /translation="MNILLLFSFILLASTVVFFLPLRLKHTWSFVIAIAAALCAGIPA
                     VEVLTGEKDFIISGFSPPGMIAPTLFIDDLSAFFILLVSLTWLTGLIYARGYLRSYFE
                     TKSAMQISLHFFSYLWLGFAMIMVAVAQDFLSFLIVWELMTLSSFVLVIFDAENRSVL
                     KAGINYLIQMHIGLLFLIAAMLIAQTVSAADEFRELGAYFSGHSNFILFLLFFTGFGI
                     KAGFVPFHSWLPKAHPAAPSHVSGVMSGVMIKMGIYGILRVLLSVENDLMYIGLFILG
                     ISLLSGILGVLQAIVQNDIKKLLAYSSIENIGIIGIGIGLGVIGLASENPVLTLLGFS
                     GALLHTLNHSLFKSMLFFAAGSVYKSTHTRNINELGGLIKKMPYTGMFFIIASIAICG
                     LPPLNGFISEYFIYSGIFSSLGNAGFYESVILILSIIGLALIGGLALFAFTKASGIIF
                     LGEARSDKAGHATEAPTSMLWPLAVVSALILAIGLVPMVFAMPVFEMIAQWFNIADSQ
                     LTSSMYIMNLQNISIVCGVFIALTTVLLVWRKLHLKNKPTEKGPTWGCGYTAPNATLQ
                     YTSTSYAEYISGIAQPVLHINKKTPVIEETDLFPVSATFETTPHDVIQEKWIDKPTGI
                     VAEWLKKMARMQTGRIEHYILYAFAFMILIFILTYFNLL"
     gene            complement(7752..8888)
                     /locus_tag="PKY63_07275"
     CDS             complement(7752..8888)
                     /locus_tag="PKY63_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPF00449.1"
                     /translation="MNFTEIRHHLHRHPELSGQEKNTAAFIIENLCQTGVTKIHYPIA
                     GHSVLAVIESRQAGPAVLFRCDTDALPIQELADHDHVSENNGVMHACGHDGHTTILLR
                     FAEMLMQRPPQSGTVLLLFQAAEETGSGARAVLRSKILDKYNISEVFALHNIPGYKTN
                     QIICRKGPFTAAVESVQISLIGKTSHASEPEKGISPAPAVADIIRYFETLNQTDKSRP
                     DYFLSTPIQIKMGADAFGTTAGEATVGYTFRAWYNTLFGQKKEEIEKGVKNTVRKTEG
                     LDVHFNWIEPFASCENNSDAVQRIMSAAEKCKFNYIIKPEPFSWGEDFGLITNEYPGA
                     LFGLGAGTNVPSLHNPDYDFPDELIESGAQMFFAIAEESMRREA"
     gene            9232..10449
                     /locus_tag="PKY63_07280"
     CDS             9232..10449
                     /locus_tag="PKY63_07280"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF00450.1"
                     /translation="MVQREKYLKNIRPFYKKPVIKVITGIRRCGKSVLLQQVRDELLA
                     SGIRSERMIAIDKELHEFDGIVNYRDLHDYVAVRSKNNSPTGYFVFIDEVQEIEHWEK
                     TINSLLAEQKYDIYITGSNARLLSSELATLLAGRYVEFQIHTLSFSEFLMLRETTSAK
                     KGNEGELFEEYLKYGGFPALHNFSWEEIASRQYLQSIYDSVILKDIIMRNQVRDVAML
                     HRILHFLADNTGNITTAKNISDYVKSLHYRIAPETVQNYIQYAVDSYILNQAKRYDVK
                     GKRLLELHEKYYLADPGLLFALTGYDPAAISQRLENVVMHEMLTRGFSVTVGKTGTYE
                     IDFICEKGNEKLYLQVCKSLYEGNASTREYRAFEGIDDHFPKYVLSMDNGFDTNRNGV
                     KWMNIKDFLLAEK"
     gene            10845..12779
                     /gene="thrS"
                     /locus_tag="PKY63_07285"
     CDS             10845..12779
                     /gene="thrS"
                     /locus_tag="PKY63_07285"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPF00451.1"
                     /translation="MVRITLPDGSVREYENAVSSMDVARSISEGLARNVLSAKVNDEI
                     WDLNRIITSDASVKLYTWDNTEGKATFWHSSAHLMAEAIEQLFPGTKFGIGPDIENGF
                     YYDMDFGANSITEADLEAIEKKMLELARTDQSLIRREVSKAEAIEHFTKKGDQYKLEL
                     INELEDGSISFYESGTFTDLCRGPHLPDTSKIKAVKLLSIAGAYWRGDEKRPMMTRIY
                     GITFPKQKELEEYLVKLEEAKKRDHRKLGKELEIFMFSQRVGSGLPIWLPKGAALRER
                     LENFLKKKQKEYGYQQVITPHIGNVELYKTSGHFQKYGKDSFQPIHTPIEGEEFMLKP
                     MNCPHHCEIYAQKPRSYKDLPVRYAEFGTVYRYEQSGELHGLTRVRSFTQDDAHLFCR
                     PDQLKREFVGVIEIVLYIFKTLGFSDYTAQISLRDPNNKEKYIGSDENWEKAESAIIE
                     ATQEQGMDTVIEIGEAAFYGPKLDFMVRDALGRKWQLGTIQVDYNLPERFDLEYIGAD
                     NQRYRPVMIHRAPFGSMERFVAVLLEHSAGKLPLWLIPDQAIVLPISEKYEAYAKKVV
                     DLVDQSEVRCLLDNRSEKIGKKIRDAELKRIPYMLIVGEKEEEQGMVSVRRQGEGDLG
                     QQTIDQFAEFIRSEIKKEIN"
     gene            12889..13353
                     /gene="infC"
                     /locus_tag="PKY63_07290"
     CDS             12889..13353
                     /gene="infC"
                     /locus_tag="PKY63_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPF00452.1"
                     /translation="MVRLVGENIETGIVSLREALAIAEEQGLDLVEISPTANPPVCKV
                     VDYRKFLYDKKKKLKEQKANATKIVVKELRLSPNTDDHDFNFKLKHAINFLQEGSKVK
                     VDVFFRGRSIVYKEQGEIILLRFANELAEYGKVESLPKLEGKRMIMIIAPKK"
     gene            13383..13577
                     /gene="rpmI"
                     /locus_tag="PKY63_07295"
     CDS             13383..13577
                     /gene="rpmI"
                     /locus_tag="PKY63_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPF00453.1"
                     /translation="MPKMKTKSAAKKRFAFTGTGKIKRKHAYKSHILTKKSTKRKRNL
                     TYFAIVDKANITSVKDMLVK"
     gene            13659..14006
                     /gene="rplT"
                     /locus_tag="PKY63_07300"
     CDS             13659..14006
                     /gene="rplT"
                     /locus_tag="PKY63_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPF00454.1"
                     /translation="MPRSVNHVASRARRKKILKRTRGYWGRSKNVWTVAKNRWEKGQQ
                     YAFRDRRAKKRSFRALWIQRINAGVRPLGLSYSQFIGLLNKSDIQLSRKTLADLALNN
                     PEAFKAVVDTLKK"
     gene            14086..15783
                     /locus_tag="PKY63_07305"
     CDS             14086..15783
                     /locus_tag="PKY63_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPF00455.1"
                     /translation="MTIQRQQKWVYAAALACLWASSEIILGSFLHNLKVPMRGTILAS
                     IAVMIMTAVGYRFKLKGIYWRAGLLTAAMKTLSPSAVLLGPMMAITIQGLLMEIVISL
                     GGTRRIMFVLGGAISITWNFAHLILGYWLYYGNDVVALGASLVQYAQKETGWQLITGW
                     GLIAAFGSVYFIAGTVAAIIGTSMKKPDSGKILHIPKKEKQTAKQVKRHDANQQPRSL
                     YWLLLLVVIVVSMLMVLSYFPLVISIFAWIAVISILIIRYRKSYRRLAKVGFWVVFLL
                     LTVLSSLLLGPTIEEGLMIGLRMNLRAILFVLVLSALSFELRNEKLAKAFNMRNKGFL
                     SILETCFETLPMVISILPPARSILKTPRESLSLFVSTINVWVDSMHFRQLEPAPAVII
                     SGEKGSGKSRLLLNRVIPELHSTKVDYAGILMQYLFDKDVHVGYTILLLPGDTEMTLV
                     GPDVNDAILSVGKYSFSRSAFEAGSKHIEQHLNSAEIIFLDESGWLETYGLGWYGILK
                     DMRLSGKPMIVVVRPDLKEDFVKFWNIDEKALLKPDSGIAEVRSALEALQENELPQA"
     gene            complement(15896..16387)
                     /locus_tag="PKY63_07310"
     CDS             complement(15896..16387)
                     /locus_tag="PKY63_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00456.1"
                     /translation="MKNIVVYLLLIILDTSCRFSDPVIGIRTNCEKGVYKGYNTVTQK
                     LSVEKTDSAHYIDTFVYYRIKKKICLDFSNKSIFAEISTREVYNSNKNNRYKFVSRKV
                     PAFMWDGWVRYYNKNKEFKQKKITRKQYRLVQCEGTGGYLKLNRDVIYNKDGTKTVTK
                     TRH"
     gene            complement(16412..17758)
                     /locus_tag="PKY63_07315"
     CDS             complement(16412..17758)
                     /locus_tag="PKY63_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPF00457.1"
                     /translation="MKNLCLLIGLMSTFAVHAQNHFIDFAGTGASSTVESVTVENLTQ
                     CASITIAGSNILNLVETVGINENHFSQSPVVSQYPNPAAENSYILYSAANAGLVTLTL
                     VNTEGKIIASRQQIVTQGTHQFSINTMPKGVYIMQLQSDNGLLSKKIISTNETAQNPV
                     ILYEGLIAEKENRSTKPLHNKSLQRNTRAIVQMQYNNGDVLKLTGKSGNYRTIMMLTP
                     TMDETVTFTFMACSDNDGNHYSVVRIGNQWWMAENLNAGTFATITTPQVAGTKFCMDI
                     NGQEDPTCPMGGLYEWNILMAGASACNGTGAAPDDDCATPVQGMCPNGWHIPSHYEWT
                     TLSRAIADNPNSFPYDMNLGVYGILEGGYLKETCSTNWWSPNLAASDKYGFGGLPGGD
                     TWDGVFEDYGQSAYFWTSSQSFSFLPWVYALNYSVSVIGRSAYVPENGFSCRCVKD"
     gene            complement(17805..18428)
                     /locus_tag="PKY63_07320"
     CDS             complement(17805..18428)
                     /locus_tag="PKY63_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00458.1"
                     /translation="MFTQFHEKKSKTKPYQNASRLLKRNDTAIAIVFALLTIAFIVIS
                     MTHETFFNWVFERHHNQWSWYIRPLFLIPFCFFAYRHSWSGISITIFCLFTSMFWFNA
                     PASVSTEVQEFLQFEKDWLYGAWDYKKLLLIFAVPVSFTALGLAFWKRSIWMGLGVVV
                     LMATGKILWSIYNAGESGKSIIIPAIVGLIICIGLIYFGFKRIEKKK"
     gene            complement(18493..19197)
                     /locus_tag="PKY63_07325"
     CDS             complement(18493..19197)
                     /locus_tag="PKY63_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00459.1"
                     /translation="MRLKQQFYQFLASGNFRSVVIDFCIVICDFFIGYIMFHYLDMND
                     EDRLLIVFGAPFIICAVAYLAGLLVGEMVIERGFFRGDENDKSESALLMNSILIGAFI
                     MVAFAASNLPMWISLTAMFLIAALWYYMHWRIMKASRKADHMPQKKYRYVAVILLFPL
                     MMCAVIFTNMLSSMITDTHDLGGNIVMVFLMMMVTWAISYVPRRFVKAVVNVKMTEGY
                     FLLFLLIDYSLKVILS"
     gene            complement(19200..19487)
                     /locus_tag="PKY63_07330"
     CDS             complement(19200..19487)
                     /locus_tag="PKY63_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00460.1"
                     /translation="MEYSLNSEFRTVIIEPNEYGLLGTTEGFRCDCSDCVGGRTNNVD
                     SSMYHITGVRILIHDTIETKTDFNNEFNWDFESAKSLGIYTAIVDSSDFEP"
     gene            complement(19647..21056)
                     /locus_tag="PKY63_07335"
     CDS             complement(19647..21056)
                     /locus_tag="PKY63_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF00461.1"
                     /translation="MTFPICIGGEFIQTAQTLDVTCSYDGSLVGTTYLAGQPQLEKAI
                     KMAEEVRAEMKEMPSWKKYDILMKSASTLRLHKDGMAKIIAGEAAKPLKLALGEVDRA
                     IQTFEVAAEESRRVAGEFMKIDWTQAGSGKEAIIKYFPAGIVSGISPFNFPLNLAIHK
                     IAPAIAAGCPIILKPSRNTPLSMLYMAQLMAESGLPKGALSVLPLDRECGNQMVTDPR
                     FAVLTFTGSPEVGWKMKTDAGKKKVVLELGGNAGVVIDKSADLNIAVPKVVAGAFAYS
                     GQVCIHTQRIFVHEEIFNSFAKAFAEATSKLRMGSPFAADTDISSMIDEANAIRVESW
                     VQDAVKDGAKILCGGKRTGASFEPTVLIDTKPEMNVCCMEIFGPVVTLEKFSDYRQAI
                     QQVNDSRFGLQAGVFTDSLDHMKYAFNTLEVGGVIINDVPTFRVDHAPYGGIKDSGLG
                     REGVKYAMMDYMEMKILVY"
     gene            complement(21236..>22492)
                     /locus_tag="PKY63_07340"
     CDS             complement(21236..>22492)
                     /locus_tag="PKY63_07340"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF00462.1"
                     /translation="YDWANSVFQLTILSAIFPIYFNSVTSHDGDSVIQVFGFDIINTV
                     AYSWSISAAYLFVALLSPFFSSMADYTGMRKSYMKFFTWVGSISCGLLYFFDPQHIEL
                     GLIAFTLATIGYGGSIVFYNSFLPVIAEHKDQDRISARGYSMGYIGGVVLLIINLAVI
                     LGQDALGIKDDSFPARIAFVTVFIWWIGFAQITFRRLPRYTFGHGKASKRVLTNGYSE
                     LRKVFRQILKSRSLTFFLFGFFFMMMGTLTVMFMAATYGEKEIGLDDTTLIATVLVIQ
                     LVGSVGAHGFAWLSGKIGNFQSLIITVFWWVFVCVAAYFITDAFGFMIVAFFIGLVMG
                     GSQALARSTYSKMLPKTTDHTSFFSFFDVMEKLATVGGTFSFGLIEAITGNMRTSVVA
                     ICIYFILSLFFFLLLYMSQKGKLRFR"
BASE COUNT         6499 a         4815 c         5007 g         6171 t
ORIGIN      
        1 tcgaacccgc gaccccgtgc gtgacaggca cgtattctaa ccaactgaac taccgaaccg
       61 tttgaacttt cccttggttt tgggactgca aatatagaaa ctttttttta attgtaacac
      121 gaccagctaa aatatttttt taattcactt accgacctat cagagacagt actccgttga
      181 tgaatgtcag cgggtgacag ggattgcccg ggacatataa gtcgacttta atattatttc
      241 ctggcgcgga tttgcaatcc gtgacaggct ctgctatttt gcggttcagc gccgggctgt
      301 ttgcaaagat gccgcccgaa acagcatcca ctccggcaag tatgcatatt ttcggggagg
      361 ggattgcttc ccaggtaatc cgtattgctt ctgccatgtt ttgcgtgatt gggccggtaa
      421 tcaccattcc atcggcgtga cgcggtgagg caacaaattc aattccatag cgtcccatat
      481 caaagttgac gttgccagaa gcattcagtt ccatttcgca cgaattgtcg ccaccggctg
      541 atacctgtcg cagcttgagc gatttacgga aaagcttgcg tacttctttg cggcgtagtt
      601 catcattaaa gcgtatgggt tctttttgtc ccggataaat cttcagcatc tcctgatcat
      661 tggttgccag tttgtagtca ttggtaaatc tgattttatc tggcaacgca aactgacatt
      721 ccttgcagaa gacgcatttg ccaaggttga gacagaatgg ctctttctca aaagcgtttg
      781 ttggacaaag cgccgaaacc atcgcctgtt cctcttttgt aatatctgtg ctgatttcag
      841 gtcgtccgcg aaacagttcg gtgagaactg cttttttcag atcaggaaca aattggagcc
      901 catgatgaat cagaataccg gtttcaggaa ttcctacata agcattcctg atatttctgt
      961 ttttttccgt ttctttttcc ggtagcaccg gaagttgcaa ttcgcgcgaa aatttcataa
     1021 tagttgttga ttataaatcg taaccacaat aactcaggtt gaaacttttg ttgcagaccg
     1081 gaaaatccga aatctcctga ttccgcacgg cgagtgccaa tccggtccaa ttgtgcagcg
     1141 aagggtcggt gattttatat tttgaaattt ctcctttttc gttggtgaca gccacatgaa
     1201 caatttcgcc gcgccatcct tctgtcatcg aaatacatac agaatccgct gccagagaca
     1261 gattgtagtt gggcgccgta tttttctcat catcattctg gcgtggattt gcaatccgtg
     1321 acagaatatc cccgacgatt gtccttatca attcaatcga cttcgatatc tcgcatctgc
     1381 gcatgagtgc gcgcgccaat acatcgccgc tggtgaatat ctcaggtttg tatttatact
     1441 ttttatatgc ctgaaatgga tgtgtggtcc gcacatcgcg cgggattccg cacatgcggg
     1501 cagccattcc cgtgagtcct gcccgtagtc cctgctcttt tgaaacgatt ccgattccat
     1561 cgaaacgcga caatgcaccg ggcatagaga aaagatgtgc agccatttcg ctatatctgt
     1621 tttcgacata ttgcaggttt ttcagaattt cattggctac ttcttttgtc atcggataat
     1681 gagttccacc ggcacggata aatcctttgc ctaagcggtt tccgcaccag tactgtgttg
     1741 tattgataac tacggtgcgc agcgattcac ataccacttg tcccaactga taagcaatat
     1801 cggcgcacaa tgccgatgta tcaccaatat gaaatgcaat tctttccagc tccaatgcaa
     1861 tggcacgctc catttgcagg gtttcatcag gtgttttacc ggccagttct tccataagct
     1921 gggcgtggca caatgaatgt ccgacagctg tatctcctgc aatgctgtcg ctgagtaccg
     1981 ctcttttgat attgctgtct gccgaaacaa aaaggtcttc aaccccgcgg tgctggtacc
     2041 ccagatgaat ttcaaggtga tagacttttt cgccgttgca gataaaacga aaatgacccg
     2101 gctcaataat gcctgcatga acaggaccta ccccaacctg gtgcaattcg atgctatcgc
     2161 tgctgaaaaa cggatatgta ttcatgcagc ttgattggtc gtaacggtta tgcggccatc
     2221 tgaccggttt cagccaggga tgccccggga aatcaacccc atgattctca tgtatttcgc
     2281 gttcaaaaac atgcatagca tttatttccg gagtcagtga ttcaagtttt gaaatttcgc
     2341 gactacattc atgcgaaaac acttgaatat ttttatttat atcatcggca acagcacaga
     2401 taaatctcca tttatcagcg ccatccggca atggaaagta ggcgagaata tggttgttct
     2461 cgtttttcat cattgcagaa acttcctgcc agaattgcac gtaatcaatc attggcaaat
     2521 ctgaaagacg aaatactgcc gggttttgat tcaataaaat agaactttcc ttcatgactg
     2581 attatttggg aagattggct atgctggcat tgatggcttc gttcagaaag tctggctgat
     2641 aaaaacaaag tactgcaaca atgatgagta aaacgaattg tggaattgtt tcaacaggtc
     2701 gggctttccc cggtgcttca gcttctgtca gtggttcggc tggatccgaa aacaggatat
     2761 gcaaaacccg ggtaagcagt gcataaatga cggctgtgag caataatgcg gtgactacaa
     2821 atgtgatcca gtgctgttgt gcaataattg atttgaaaat cagaatttcg ctgatgaaaa
     2881 ttcctgaggg tggaatggca agaataaaaa ccacgcaaca aagcaataat aatccgcctg
     2941 ccgggtagct tttcatataa ttcccaactt gtttcaatgt ataggaatga gtaacatttt
     3001 tgaattgccc catgtggaaa aagatggaac ttttcaccag tgaatgaagc agaataagga
     3061 gaatggcagc ataataacca cttttaccac tccccattgc tatagccaca agtcccatca
     3121 tttcaagact tgaataggca aacatgcgtt tgatgtgata tgctttcagc atatatccgg
     3181 cggcaatcag caacgaaaga attcctgtca gcaccagtga atgttgcatg aagggcagta
     3241 tttcggttga agaaaacaag gtgaatactc tgaaaatggc caggaatccg acgttcataa
     3301 gcgtagtcga aatgagtgca ctcaccggtg cgggtgctac tgagtgcgca tcaatggtga
     3361 cagaatgcat gggaaacaat cccatttttg tgctgaatcc gacaaggaca aatacaaagg
     3421 caacttttaa gtaaatggga ttggcctgac ttaccaacag ggagaactcg tcaaaagtga
     3481 gattggctgc atcgtagcga ccataaataa aaccaagcag aagtattccg acataggcaa
     3541 ttgaaatacc caccgaacag ataaatacat atttccacgt agcctcgagt gattgtggtg
     3601 tgcgatcgtg atatatcagt gcggcaaccg acagggttgt ggcttcaaca aatacccaca
     3661 acatggtcat tgaatcagaa agatatgcac ctgtcatgaa agtcatgaga gccatcattg
     3721 aaatatgata aatgttgaaa tgctttaccg atgcgttttg tgtgtatcgg aagccatgat
     3781 aaaatgttgg cagcgagagc agtgccagtg cactcagcag cagcaatcct tcgtggctga
     3841 aggtgaagaa atggacagga gattcatcaa tatgcatcca tccatgaatc gttaatccga
     3901 taagagaaac agagaacagt agtgaggctc cgcgtgtaaa gagtttgctg cgcatgaaaa
     3961 aaatggcgag agcaaaaaac agcgttaagg aaaaaaataa aatcagttcc atagttttaa
     4021 tcctttaatt gggtaagctc gccggtatca gggttcaggc gcagcccgaa gaatcccata
     4081 atgagaactc cgacaaaaat atcaagcaac atggcaatat tgatgagtgc cggcatttcg
     4141 tttcccaacg caatagtaaa aagaaaaacg gcattttcaa taatcaggaa tccaatcagg
     4201 tgggaaataa tcaatttgtg cgtgacaatg atgataagac cgctcaaaag cgcatacaga
     4261 gccactgtca taaaaacaat attgataaat ggattttgca atgtgtatgc aatgatgacc
     4321 gaaacaataa ggcttattgc gtttaaaagt agtatgtaaa acgacggcgt ggcttgcgca
     4381 tgtactttaa aaacctgtgt acgccgaatg atgtagaaaa gcatgaaagg gattacaatg
     4441 cccttgaata caagtgtttc ggacactaca agaatcaggt tgccggtggc catctcgttc
     4501 agttcgagaa aagcaatgcc gaaaatgagc accccttgca cacccatgaa gcgcgcaaac
     4561 acacggaacc gttgtgctgc tgaaagataa atcagtgttg cggcaaaggc aacaatcaga
     4621 aattctatca tgataaaatc attttttcag ttaatatgag tactaccaca aaggccagaa
     4681 gtgccatgga ggagatcgtg aacaaaaatg ccggatttct gccaagcttg tgccgggcct
     4741 tgaatgattc gataaaacct atatacactg ccatgaaaaa tataaccaga gcaaacaatg
     4801 ccagttgaat ttcaatgttc cattgaatgg gaatcagtac gtcaaaaatc agcgtggaat
     4861 acagcgcaaa tttcagccat gttgttatat ggatgagtgc cttgtcgaat ccggagtaat
     4921 caagaatcat tacttcgtga atcatggtga gctcaagatg cgtctttgga tcatcaacag
     4981 gcaggcggct gttttcgact tgcataatct gagccagaat aatcacactc aggatgctgt
     5041 agataagaat gtaggaattg tcaggattgt agagaccaac caatatttca ttaaatgatg
     5101 tatatccggt gagcatggcc agtgttccga tgagaataaa aaatgcaggt tcagtcagta
     5161 atgaatacag tgcttcacgg ttggctccca tcccttcaaa tgcagaaccg gtatcgagtg
     5221 ctgcaagaat cagaaaaaac tttccaagcg caagcatata ggcaaaaaac acgatgtcgc
     5281 cattgaatga cagcatagat ggaaaagggc cgaatggcaa taccagtaat gcactcagca
     5341 ccgaagccaa tccgatggcc ggggctattt taaagacggc actggtagtg gtgcttatga
     5401 cattcccttt ttggaaaagc aatgcaatat ctttgattgg ctgaaataat ccggggcctt
     5461 tgcgaccggc aaaaatactt ttggagcgca gtattattcc cgggaaaaaa agtgcagcaa
     5521 tcagaataaa tattatagtc atcatgatca gagcagatta aaataggtta atataaatat
     5581 taaaatcatg aatgcaaatg cgtaaagaat gtaatgttca atacgccctg tttgcatgcg
     5641 ggccatcttt ttgagccatt ccgcaacaat tccggttggt ttgtcaatcc atttttcctg
     5701 aataacatcg tgcggtgttg tttcaaatgt ggccgatacc ggaaacagat cagtttcttc
     5761 aatgaccggt gttttcttgt ttatatgtaa aacaggttgc gcaatccctg aaatatattc
     5821 agcatacgaa gtagatgtat attgcaaagt ggcatttggt gcagtatagc cgcatcccca
     5881 ggttggacct ttttcggttg gcttattttt gagatgcagt ttgcgccata cgagcagaac
     5941 agttgtcagc gcaataaaaa caccgcaaac aatactgatg ttctgcagat tcataatgta
     6001 catggatgat gtaagctggc tgtcagcaat attaaaccat tgcgcaatca tttcaaaaac
     6061 aggcatggcg aaaaccattg gtacaagtcc gatggcgagt atcaaagcag aaacaacagc
     6121 gagtggccac aacattgatg ttggcgcttc agttgcatgt ccggctttat cactgcgggc
     6181 ttcacccaga aaaatgatgc cggaagcttt ggtaaaggca aacagtgcca acccgccgat
     6241 tagagccagt ccgatgattg aaaggatcaa tatcacggat tcataaaaac cggcattgcc
     6301 cagacttgaa aagatacctg aataaataaa gtattcagaa atgaaaccgt tgagtggagg
     6361 caatccgcag atggctatgg atgcaataat gaagaacatt cccgtgtaag gcattttttt
     6421 tatcagtccg ccgagttcat tgatgttgcg ggtatgcgta gatttataaa cagatccggc
     6481 tgcaaaaaac aacatggatt tgaacagtga gtgattgagt gtatgcagca gcgctccgct
     6541 gaacccgagc aatgtaagta ctgggttttc gctggccagt cctataaccc caagcccgat
     6601 tcctattccg ataattccga tattttcaat gctgctgtat gccagcaatt tttttatatc
     6661 attctgaacg atggcctgca gaacgcccag aatgccgctc agcaaggaaa tgccaagaat
     6721 aaacaatcca atatacatta aatcattttc cacagacagc aatacgcgca ggattccata
     6781 aatacccatt ttaatcatta cgcccgacat cacacccgaa acgtgtgaag gtgcggccgg
     6841 atgcgctttt ggtaaccatg aatgaaaagg aacaaatccg gctttgatgc caaaacctgt
     6901 aaaaaaaagc aggaataaaa tgaaattgga gtgcccggaa aaataggcac ctaattctct
     6961 gaattcgtct gccgctgaaa ctgtctgcgc aatcaacatg gccgcaatca gaaacagcaa
     7021 tccaatgtgc atctgaatga ggtaattgat gccggctttc agcaccgaac ggttttccgc
     7081 atcaaaaatt accaatacaa acgatgacag ggtcatgagc tcccagacaa tcagaaacga
     7141 cagaaaatcc tgtgcaacag caaccatgat catggcgaaa cccagccaca gataagaaaa
     7201 gaaatgcagc gaaatttgca tggctgattt ggtttcgaaa taggaccgga gatatccccg
     7261 cgcgtaaatc agcccggtga gccatgttaa gctgaccaaa agaataaaaa atgcggagag
     7321 atcgtcgata aacagcgttg gtgctatcat tcccggaggg ctgaagccgg atataataaa
     7381 atctttttct ccggtgagta cttcaacagc gggaattcct gcacacaaag ccgccgcaat
     7441 cgcaattaca aatgaccatg tatgtttcaa tcgcagtggg agaaaaaata caacggtgct
     7501 tgctagcagg ataaaactaa acagtagtaa tatattcatg actttcgttc acgatttttg
     7561 cggcaaaatt agttatatta aaattgaccg ggtataaaat gtcttttggt tatcaacagg
     7621 ccacggtgta ttcagcattc agccttcagt ctgtctgcta tttagtccgc cgctgcggtg
     7681 gatttatatt ttcttccact ggcgcggata ttccacccgt gacagtgcta tctgtcccgc
     7741 attcgttctg cctatgcttc gcgccgcatg ctttcttctg caatggcgaa aaacatctgt
     7801 gcaccgcttt caatcagctc atccggaaaa tcatagtccg gattatgcag cgaaggcacg
     7861 ttggttccgg ctcccagtcc gaaaagcgca ccaggatatt catttgtaat cagtccgaaa
     7921 tcttcgcccc aggaaaatgg ctccggtttt ataatgtaat tgaatttaca tttttcagca
     7981 gcagacataa tgcgctgaac cgcatctgaa ttattctcgc aggatgcaaa aggttctatc
     8041 cagttgaaat gtacatccag tccttctgtt ttgcggaccg tgtttttcac tcctttttcg
     8101 atttcctctt tcttttgacc aaacaaagtg ttgtaccatg cgcggaaagt atatccgaca
     8161 gtggcttccc cggccgttgt gccgaatgca tccgcaccca tttttatttg tatcggagtg
     8221 ctcaggaaat aatccggccg gcttttatcg gtctgattca gtgtttcgaa atagcggatg
     8281 atatctgcca cggccggtgc aggactgatg cccttttcgg gttccgatgc atgactggtt
     8341 ttgccgatca gcgaaatctg cacactctct accgccgctg tgaatggccc tttgcgacat
     8401 ataatctgat tggttttata acccggaata ttgtgcagcg caaaaacttc cgaaatgttg
     8461 tatttgtcta atatttttga acgtagcacg gcgcgcgccc cgcttccggt ttcttcggct
     8521 gcctgaaaca acaacaaaac agtaccgctc tgtggcgggc gctgcatgag catttcggca
     8581 aaacggagca atatggtggt gtgtccgtcg tgaccgcagg cgtgcatcac tccgttgttt
     8641 tccgacacat gatcgtgatc ggccagttcc tgaatgggca atgcatcggt gtcgcagcga
     8701 aacagaaccg cgggtccggc ctgcctgctt tcaattacag ccagcaccga atgtccggct
     8761 atcggataat gaatctttgt aacgcccgtc tggcacaggt tttcaataat aaaagccgct
     8821 gtattttttt cctgtcccga caactcggga tgacgatgca ggtggtgacg tatttccgta
     8881 aagttcataa atgacaaatc agcttacaaa gataatatgc aagaatgcaa cagacaacaa
     8941 gaattttgaa gttcattttt caattctgct caattacagg cgatggagtt accggccgca
     9001 gaatggtcac cagacattac tgatagatga tagcattttc aggatattga gtcatgagat
     9061 tatactcaat ggtttaccaa aaagggatga agaattgtca tataccattt ccgcaaaaat
     9121 ttctatgaga gatgtttcag gtataaatga aactcgttag atgttttaag aaagattcag
     9181 atataactga ataaatatat atctttgtaa aaccaaattt gtaaaacaga catggtacaa
     9241 agagaaaaat atttaaaaaa cataaggccg ttttataaaa aacctgtaat caaggtcata
     9301 accggcattc ggcgttgtgg caaaagcgta ttgttgcagc aggtgaggga cgagttgctt
     9361 gcatccggca ttcgttctga gcgaatgata gccattgaca aagagctgca tgagtttgac
     9421 ggtattgtga attaccgtga cctgcatgat tatgtggctg tacgctcaaa gaacaacagc
     9481 ccgacaggtt attttgtttt tattgatgag gtacaggaaa ttgagcattg ggaaaaaaca
     9541 atcaattcat tgctggctga acagaagtac gatatttaca tcaccggttc aaatgcccgc
     9601 ctgctttcat cggaactggc tacattgctg gctggccgct atgttgagtt tcagattcat
     9661 actctgagtt tttctgaatt tctgatgtta agagaaacca cgagtgctaa aaagggcaat
     9721 gaaggcgaat tatttgagga gtatttgaag tacggggggt ttcccgcttt gcacaatttt
     9781 tcgtgggaag aaatagcaag caggcagtat ctccagtcca tctatgattc ggtaattttg
     9841 aaagatatta ttatgcgcaa tcaggtgcgg gatgttgcca tgctacaccg cattcttcac
     9901 tttctggctg ataacactgg gaatatcaca acggcaaaaa atattagtga ttatgtaaag
     9961 tcgttgcatt accgcattgc cccggagacc gtgcaaaatt acattcagta tgcagttgat
    10021 tcctacattc tgaatcaggc aaaacgctat gatgtaaaag gaaagcgctt acttgaactt
    10081 catgagaagt attatctggc cgatccgggc ttactgtttg cgttgaccgg ctatgatccg
    10141 gcagcaatat ctcaaaggct tgaaaatgtt gttatgcatg aaatgctaac cagaggattc
    10201 agcgtaactg tgggcaaaac aggtacgtat gaaattgatt ttatttgcga aaaaggaaac
    10261 gaaaagctat accttcaggt atgcaaatct ctttatgaag gcaatgcttc tacaagagaa
    10321 tacagagcat ttgaaggaat agacgatcat tttcctaaat atgttttatc gatggataat
    10381 gggtttgata ctaaccgcaa tggggtaaaa tggatgaata taaaagattt tttactggct
    10441 gagaaatagc aacatgggcg tagtcctgtc ccgacaactc gggatgacga tgcaggtgtt
    10501 ggcggatggt ggggtaggtc ataaaaaatg aatctgagag caaagataat atcgttatgt
    10561 atcaaaaatc caattttctt tacataggtg catggtaatg tatagaaaat tcaattttct
    10621 ttacaaagca aacccgtcat gtaaagatct tgcgattttc tttacaaagc aaacctgaaa
    10681 tgcatgtttt tttgaattta tctacaaaga agaattattg ctttccgaat tttcaaattc
    10741 tcacatcagc acattttcac atctccacat ttccacatta ttaattgttt ggtcaattca
    10801 atgattattt gtacttttgc acctcttttt gaaaactgaa aaaaatggtc cgaattactt
    10861 tacccgatgg cagtgtgcgt gaatacgaaa acgcagtgag cagcatggat gtagcccgta
    10921 gcattagcga agggcttgcc cgtaacgtgc tttcggcaaa agtgaacgat gaaatatggg
    10981 atctgaaccg cattatcact tccgatgcca gtgtgaaact ttatacatgg gacaataccg
    11041 aaggcaaagc cactttctgg cacagctcgg ctcacctgat ggctgaagcc attgaacagt
    11101 tgttcccagg cacaaaattt ggcattggtc ctgatatcga aaacggcttt tactacgaca
    11161 tggatttcgg cgccaacagc attactgaag ccgatctcga agccattgaa aagaaaatgc
    11221 tggaactggc gcgtactgat cagtccctga tccgccgcga ggtttcgaaa gccgaagcca
    11281 ttgagcattt tacgaaaaag ggtgaccaat ataaacttga actcatcaac gagctcgaag
    11341 acggctcaat ttcattttac gaaagcggta catttaccga tctttgtcgt ggaccgcatc
    11401 tgcccgacac ttcaaagatt aaggcggtga aactgctttc cattgccggc gcttactggc
    11461 gtggcgacga gaaacgtccg atgatgaccc gtatttacgg cattaccttc ccgaaacaga
    11521 aggaattgga agaatatctt gtaaaactcg aagaagccaa aaaacgtgat caccgcaagc
    11581 ttggaaaaga attagaaatt ttcatgttca gtcagcgcgt tggttcaggg cttccaatct
    11641 ggctgccgaa aggcgccgca ctgcgtgaac ggctcgagaa ctttttgaag aaaaagcaaa
    11701 aagaatacgg ctatcagcag gtgataacgc cgcatatcgg aaacgttgaa ctgtacaaga
    11761 cttcaggtca ctttcagaaa tatggtaaag attcgttcca gcccattcat acgccgattg
    11821 aaggtgaaga attcatgctg aagccaatga attgcccgca tcattgcgaa atttacgcac
    11881 agaagccacg tagttacaag gatctgccgg tgcgttatgc tgaattcggt acggtgtatc
    11941 gttatgagca aagcggcgaa ttgcacggac tgacccgtgt acgcagcttt acacaggatg
    12001 atgcgcattt gttctgtcgt cccgatcagc taaaaagaga atttgtcggg gtgattgaaa
    12061 ttgtacttta cattttcaaa acactgggtt tcagcgatta taccgctcag atttcgctgc
    12121 gggatccgaa caacaaggaa aaatatatcg gttcggacga gaactgggaa aaagccgaaa
    12181 gcgctattat agaagccact caggaacagg gcatggatac ggtcattgaa attggcgaag
    12241 ccgcatttta cggtccgaag ctcgatttca tggtacgcga tgccctcggc cgcaagtggc
    12301 agctgggaac gattcaggta gattataatt taccggaacg tttcgatctg gaatatatcg
    12361 gagccgacaa tcagcgctat cgtccggtga tgattcatcg tgccccattt ggctcaatgg
    12421 agcgttttgt ggctgtattg ctcgaacata gtgcaggcaa acttccgctt tggctcattc
    12481 cggatcaggc cattgtattg ccgatcagtg agaaatacga agcatatgca aaaaaagtcg
    12541 tagatttggt cgatcaatcc gaagttcgtt gtctgttgga taatcgttcc gagaagattg
    12601 gaaagaaaat ccgtgatgct gagctgaaaa ggattccata tatgcttatt gtcggagaga
    12661 aagaagagga gcagggtatg gtatcggttc gccgtcaggg agaaggtgat ctgggacaac
    12721 agacgattga tcagtttgct gaattcatac gcagcgaaat aaaaaaagaa attaactaat
    12781 ataggaggta aagctatagc aacgaacttt ccatttaaag gacccatccg tcggagaccc
    12841 ccgcaaaggg aggaaccgta caaaatcaac gagtttatca aagcacccat ggtgcgactg
    12901 gtaggcgaaa acattgaaac cggcattgtt tcactacgcg aagcattggc aatagctgag
    12961 gagcagggat tagacctggt cgaaatatct ccaactgcta atccaccggt ctgcaaagtt
    13021 gttgattatc gcaaatttct ttacgacaag aagaaaaagc tgaaggaaca aaaagccaat
    13081 gccactaaaa ttgttgttaa ggaattgcgt ttgagcccca atacagatga ccatgatttc
    13141 aactttaagc tgaaacatgc cattaatttt cttcaggaag ggtcaaaagt aaaagtggac
    13201 gtatttttcc gcggacgttc gattgtgtac aaggagcagg gcgaaatcat tctactgcgc
    13261 tttgccaatg aacttgctga atatgggaaa gttgaatcac tcccaaaact tgaaggtaaa
    13321 aggatgatta tgatcatcgc tcctaaaaaa taaaaatctc aacccaaata ataattacga
    13381 tcatgcctaa gatgaaaacg aaatccgctg ccaaaaaacg ctttgcgttt acaggtaccg
    13441 gtaaaatcaa gagaaagcat gcctacaaaa gtcacattct gacaaaaaaa tcgacaaaac
    13501 gcaaacgtaa tctgacttat tttgccattg tcgataaggc caacatcact tcggtgaaag
    13561 acatgcttgt gaagtaaaac aaatcgttta accttgctgt cagcatgaaa gatttcgcgt
    13621 aaggcgaaac gctgaagcaa ctaaagaaag gagaaaaaat gccacgttca gttaatcatg
    13681 ttgcgtcgcg tgcgcgcaga aaaaaaatcc tcaagcggac ccggggttat tggggtcgta
    13741 gtaaaaatgt gtggaccgta gcgaaaaatc gctgggaaaa aggtcaacaa tatgcttttc
    13801 gcgatcgcag agccaagaaa agaagttttc gtgctttgtg gattcagcgt atcaatgctg
    13861 gtgttcgtcc gctcggactt tcatactcac agtttattgg gctgcttaac aaatccgata
    13921 ttcagctcag ccgcaaaact cttgctgatc ttgcgctgaa caatccggaa gcttttaaag
    13981 cggttgttga tacattgaaa aaatagacgc aattttctaa tcaacatatt gaaaactgtc
    14041 catctcgggc agttttctta tttttgtcga aatgcaaaat attacatgac gatacaacga
    14101 caacaaaagt gggtttatgc ggctgctttg gcttgcctgt gggcttcctc cgaaattatt
    14161 ctcggtagct ttctgcataa tcttaaagtc ccgatgcggg gaaccattct ggcttccatc
    14221 gcggtaatga ttatgactgc agtcggatat cgctttaagt taaaaggcat ctactggcgt
    14281 gctggcttgc tcactgcagc catgaagaca ctgagtccca gcgcggtttt gctggggccc
    14341 atgatggcta tcaccattca ggggctttta atggaaattg tcatttcttt gggtggaaca
    14401 cgtcgtatca tgtttgttct tggaggggct atttcaatta catggaattt tgcgcatctt
    14461 attcttggtt attggctcta ttacggaaac gatgttgtag cgcttggtgc aagtcttgtt
    14521 caatatgcac agaaagaaac cggttggcag ctcattactg gctggggact cattgccgct
    14581 tttggttctg tatattttat cgctggtact gtggctgcaa ttatcggaac cagtatgaag
    14641 aaacctgatt ccggtaaaat actgcacatc ccaaagaaag aaaagcaaac agcaaaacag
    14701 gtgaaaagac atgatgcaaa tcaacaacca agatcgctct actggctgct tttgcttgtt
    14761 gtaatcgttg tttccatgtt gatggttttg tcttatttcc cgcttgtaat tagtatattt
    14821 gcgtggattg ccgttatttc cattttgatt attcgttatc ggaaatctta tcgccgtctg
    14881 gcaaaagttg gtttttgggt ggttttcctt ttacttacgg ttttgtcgtc tttgctgctt
    14941 ggccctacga ttgaagaagg tctgatgatt ggtctgcgca tgaatctgcg tgccattttg
    15001 tttgttctgg tattatcggc actgagtttt gagctgcgca acgagaaact tgccaaggca
    15061 ttcaacatgc gcaataaggg attccttagc atacttgaaa cctgttttga aactcttccg
    15121 atggttattt ccattctgcc tccggccaga tctattttaa aaactccacg cgaaagttta
    15181 tctttatttg tgagcaccat taatgtttgg gtcgattcca tgcatttccg gcagctggag
    15241 ccggcgccgg cggttatcat ttccggcgaa aagggaagcg gcaagagtag gttacttttg
    15301 aatcgggtta tacctgaatt acacagcaca aaagttgatt atgccggaat tctgatgcaa
    15361 taccttttcg ataaagatgt acatgttggt tataccattt tattgctacc gggagatact
    15421 gaaatgacgc ttgtgggacc cgatgttaat gatgccattc tgtcagtcgg caaatatagt
    15481 ttttcgcgtt ctgcattcga agcgggctcg aagcatattg aacaacatct gaattcggcc
    15541 gaaattattt ttcttgacga aagcggttgg ctcgaaacct acggtctggg ctggtatggc
    15601 attttgaaag acatgcgtct gagcggaaaa cccatgattg tggttgtgcg ccccgatctg
    15661 aaagaagact ttgtgaagtt ctggaatatc gacgaaaaag cgctcctgaa gccggattca
    15721 ggaattgccg aagtgcgcag tgcgcttgag gctttgcagg aaaatgagct gccgcaggca
    15781 tgaaatagct gcgaagttca aaaaacgctg ccggtgctgc agaggatgga gagctgtcac
    15841 acgtgaattg tggttgagat tgatttgttt ttgttcttac cttcattctt gaaaatcaat
    15901 gcctggtttt tgtaactgtt ttcgttccat ctttattata gataacgtcc ctgtttaatt
    15961 tgagataccc accagtacct tcacattgaa ccaaacgata ttgctttctc gtaatttttt
    16021 tctgcttaaa ttctttgttt ttgttatagt atctaaccca gccgtcccac ataaacgcgg
    16081 ggactttcct tgaaacaaat ttataccgat tgtttttgtt tgaattatat acttctcttg
    16141 ttgagatttc agcaaaaata cttttgttag aaaaatcgag acatattttt ttcttgattc
    16201 tataatatac aaatgtgtca atatagtgag cagagtcggt tttttcgacg ctcaactttt
    16261 gtgtaacagt gttatatcct ttatacacgc ctttctcgca gtttgttcta atgccaataa
    16321 ccggatcgct aaaccggcat gaggtgtcga gaatgattaa aagcaaataa acaacgatat
    16381 ttttcatgtt tcttgtttga caaaagaatt ttcagtcttt cacacaccgg cagctgaagc
    16441 cgttttcagg cacatacgcg gaacgcccaa tgaccgaaac gctgtagttt aatgcatata
    16501 cccatggtaa aaatgaaaac gattgggagg atgtccagaa atacgcgctt tgtccataat
    16561 cttcaaaaac gccatcccat gtgtcgccgc cgggcaatcc cccaaaaccg tatttgtccg
    16621 atgcagcaag gttgggtgac caccagtttg tcgaacaggt ctctttcaga tagccgcctt
    16681 ccagaatgcc atacacaccc agattcatat cgtatggaaa actattcgga ttgtcagcga
    16741 tggcgcggct cagcgttgtc cattcataat gcgatggaat gtgccagcca ttcggacaca
    16801 tgccctgaac gggggttgca caatcatcgt caggcgcggc tccggttccg ttgcatgcgc
    16861 ttgcgcctgc cataagaata ttccactcgt acagtccgcc catgggacaa gtcggatctt
    16921 cctgtccgtt gatatccatg cagaattttg ttcctgcaac ctgcggtgtt gtgatggtgg
    16981 caaatgttcc tgcattcagg ttttcggcca tccaccactg attgccaatg cgcacaacgc
    17041 tgtagtgatt tccgtcattg tcggagcagg ccataaaagt aaaagtgacc gtttcatcca
    17101 tcgttggtgt gagcatcatt attgttctgt aattacctga ttttcctgtc agtttgagta
    17161 cgtcaccatt attgtactgc atttgaacaa tagctctggt attgcgttgc agagatttat
    17221 tatgtaatgg cttcgtactt ctgttctctt tttcagcaat cagaccttca tatagaataa
    17281 ccggattttg ggctgtttcg tttgtactga ttattttttt agaaagcaaa ccattgtccg
    17341 attgcagctg cattatatat actcctttcg gcatagtgtt gatgctgaac tggtgtgtgc
    17401 cctgtgtgac tatttgttgt ctggaagcaa tgatttttcc ttccgtattc accagagtaa
    17461 gtgttaccaa accagcattt gctgccgaat acagtatata gctgttttct gcggcaggat
    17521 ttggatattg actcacaaca ggcgattgtg aaaaatgatt ttcattgatt ccaactgttt
    17581 caaccagatt cagaatattg ctgcctgcaa tagtaatgct ggcgcattgt gtaagatttt
    17641 caacagtaac cgattccacc gtgctgctgg cacctgtccc tgcaaaatcg atgaagtgat
    17701 tctgtgcatg aacagcaaaa gtactcatga gaccaatgag aagacaaagg tttttcattg
    17761 ttggtgtgtt ttgtttgata gtgcgttttc tgagattatt tcgtttattt tttcttctca
    17821 attcttttaa atccaaaata aatcagtcca atgcaaataa ttagtccaac gatggccgga
    17881 atgataatgg atttgcccga ttcacctgcg ttgtagatgc tccagagtat ttttcctgtg
    17941 gccattaaaa caacaacacc caatcccatc cagatgctgc gtttccagaa tgcaagcccc
    18001 agtgcggtaa atgaaaccgg tactgcaaaa atcagcagaa gctttttata gtcccatgca
    18061 ccatacagcc aatctttttc gaattgcaga aattcctgta cttcggtgct gacgctggcg
    18121 ggcgcattga accagaacat gctggtgaac aaacaaaaaa tggttatgga tattccgctc
    18181 cagctgtgcc ggtacgcaaa aaaacaaaac ggaatcagaa acaacggccg tatataccag
    18241 ctccattgat tatgatgccg ttcgaaaacc cagttgaaaa atgtttcatg cgtcatcgaa
    18301 atcacaatga atgcgatggt cagcaatgca aaaacgatgg caatggcggt gtcgtttctt
    18361 tttaataagc gacttgcgtt ttgatacggt tttgttttgc tttttttttc atggaattgt
    18421 gtaaacaaat atattaattt tcctgcaggc aaatataatt tgagtgaatc aagggaattc
    18481 agctcagttc tctcagctca gaatcacttt cagcgaataa tcaatcaaca gaaaaagcag
    18541 aaaatatcct tcagtcattt ttacattcac cacggctttc acaaagcgtc gcggcacata
    18601 gctgatggcc caggtgacca tcatcatcag aaacaccatg acaatattgc ctccgaggtc
    18661 gtgggtatct gtgatcattg acgacagcat gttggtgaaa atgacagcgc acatcataag
    18721 cgggaataaa agaatcacgg cgacatatct gtattttttc tgtggcatgt gatccgcttt
    18781 gcgactggct ttcattatcc gccagtgcat gtaataccag agtgctgcaa tcagaaacat
    18841 ggctgtgagc gaaatccaca tgggcagatt ggaagcggcg aaggcgacca ttataaatgc
    18901 accgatcaga atggagttca tcagcagggc tgattcggac ttgtcatttt catcacctct
    18961 gaaaaagcca cgttctataa ccatttcgcc gacaagcaat ccggccagat aggccaccgc
    19021 gcaaatgatg aaaggtgctc cgaacacaat cagcaagcgg tcttcgtcat tcatgtcaag
    19081 ataatgaaac atgatataac caataaaaaa atcgcaaatc acaatgcaga aatcaatcac
    19141 caccgaccta aaattgccgg aggcgagaaa ttgatagaat tgctgtttaa gtctcatggt
    19201 cagggttcaa aatcagagct gtcaacaatt gcagtgtata tccccagact ttttgcagac
    19261 tcaaaatccc aattaaattc attattgaag tctgtttttg tctcaattgt atcatgaatt
    19321 aatattcgca caccagtgat gtggtacatg gatgaatcaa cgttgtttgt tcttccgccc
    19381 acgcaatctg agcaatcaca gcgaaatcct tcggtagttc ccagcaatcc atactcattt
    19441 ggttcaatga ttacagtcct gaattcagag ttaaggctgt attccacttt caaaagttga
    19501 gtagagttgt tataaatctt aaactcatac tcaaaataac aatcacagct gcataatgca
    19561 acaatgctaa cggtacaaag ggaaaataca agttttggaa atttcatatt tcgatttctt
    19621 ttacttatat aaaattataa attatttcaa tacaccaata tcttcatctc catataatcc
    19681 atcattgcat atttcactcc ttcgcggccg aggccactgt ccttgatgcc accgtagggc
    19741 gcatgatcga cgcggaaagt cggtacgtca ttgatgatca caccgcctac ttccaatgta
    19801 ttgaaagcat atttcatgtg atcgagcgaa tcggtgaaaa cgcccgcctg tagcccgaag
    19861 cgcgagtcat tcacttgttg tatcgcttgt ctgtaatcgc tgaatttttc caatgtcact
    19921 accggaccga aaatttccat gcagcacaca ttcatttccg gtttggtgtc gatgagtact
    19981 gtaggctcaa acgaagcacc cgtgcgcttt ccgccacaga gaattttcgc accgtctttc
    20041 acggcatcct gtacccagct ctccacgcgg atggcattgg cttcgtcaat catggacgaa
    20101 atatcggtgt cggccgcaaa cggtgagccc atcctgagtt tcgatgttgc ttcggcaaag
    20161 gctttggcaa atgaattaaa aatttcttca tgcacaaaaa tgcgttgcgt gtgaatgcac
    20221 acctgccctg aataggcgaa tgcacctgcc acaactttgg gaactgctat attcagatcg
    20281 gcgcttttgt caatcacaac gccggcattg ccgccgagtt cgagcacaac ttttttctta
    20341 cctgcatcgg ttttcatttt ccagcctact tcgggcgagc cggtaaatgt cagcacggcg
    20401 aagcgcggat cggtcaccat ctgattaccg cattcgcggt cgagcggcaa caccgacagc
    20461 gcacctttcg gaagtccgct ctcagccatc agctgcgcca tatacaacat agacagcggt
    20521 gtattgcgcg atggtttgag tatgatggga caacctgctg caatggcagg cgcaatcttg
    20581 tgtatagcta agttcaatgg aaaattgaac gggctgattc cggatacaat gccagccgga
    20641 aaatatttta taatggcttc tttgccgctg ccagcctgcg tccagtcgat tttcatgaat
    20701 tcgcctgcca cgcggcggct ttcttcggcg gccacttcaa aagtctgtat ggcgcgatcg
    20761 acctcaccca aagccagctt taagggtttt gccgcttcgc ctgcaataat tttcgccatt
    20821 ccatctttgt gtaaacgcag tgttgaagcg cttttcatca gtatgtcgta ctttttccac
    20881 gatggcattt ctttcatttc tgcccggact tcttcggcca ttttaatggc tttttcgagt
    20941 tgaggctgtc cggccaggta ggttgtacca accaatgaac cgtcgtagga gcaggttaca
    21001 tccagtgttt gtgcggtctg tataaattca ccgccgatgc atatggggaa ggtcatgatg
    21061 aattgttttt gcaaatataa tattagatgt gttaatcacg tgaaacgtga caagtgtgcg
    21121 aatatgctga tgtcctaatt gggcaatatg ctgctgttat gatttggaaa tttataatca
    21181 ttgttcgaac gcttctttat tggaggattc ggataaatgc aatacaattc agaatttatc
    21241 tgaatctcaa ctttcctttc tgcgacatat agagcaacaa aaagaaaaac agactcagaa
    21301 taaaataaat acaaatggct actaccgatg tgcgcatgtt tccggtgatg gcttcaatca
    21361 atccgaagct gaacgttccg ccaaccgtgg ctagtttttc catcacatcg aagaagctga
    21421 aaaacgatgt atgatcagtt gttttcggga gcattttgga ataggtcgac cgcgccagtg
    21481 cctgcgaccc gcccataaca agaccgatga aaaaggcaac aatcataaat ccaaatgcat
    21541 cggtaatgaa gtatgccgca acacaaacaa acacccacca aaagacagta ataatcagcg
    21601 attgaaagtt cccgattttt ccggaaagcc atgcaaagcc gtgtgcgcca accgatccga
    21661 ccaactgaat aacaagtact gttgcaatga gtgttgtatc atcgagtccg atttcttttt
    21721 cgccgtaagt ggctgccata aacatgacgg tgagggttcc catcatcata aagaaaaatc
    21781 cgaaaaggaa aaaggtcaga ctgcgcgatt tcagaatctg gcggaatact ttcctgagtt
    21841 cgctgtaacc attggtcagc acgcgtttgc tggctttgcc atgtccgaaa gtgtatctgg
    21901 gtaatcgacg aaaagtgatt tgtgcaaatc cgatccacca gataaaaacg gttacaaatg
    21961 caatgcgtgc gggaaatgaa tcgtctttta tgccaagtgc atcctgacca agaatgactg
    22021 ccagattgat aatgagcaac accaccccgc caatgtatcc cattgaataa ccgcgggcgc
    22081 tgatgcggtc ctgatctttg tgttcggcaa ttaccggcag aaaggaattg taaaacacaa
    22141 tgctgccgcc ataaccaatg gtggccagtg taaatgcaat cagtccaagc tcgatgtgct
    22201 gcggatcgaa gaaatacagc agtccgcatg aaatggatcc gacccaggtg aaaaatttca
    22261 tgtacgattt tcgcatcccc gtgtaatcgg ccatggatga gaaaaacggc gacaacaatg
    22321 ctacaaataa ataggctgcc gaaatcgacc acgaatacgc aaccgtgttg atgatatcaa
    22381 aaccaaacac ctgaataacg ctgtcaccgt cgtgactggt cacactgttg aaataaatcg
    22441 gaaatatggc tgaaagaatg gtgagctgaa atactgaatt ggcccagtcg ta
//
LOCUS       DAOPKE010000046        21862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2543_length_21862_cov_13.602651, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000046 DAOPKE010000000
VERSION     DAOPKE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_21862_cov_13.602651"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKY63_07345"
     CDS             complement(<1..288)
                     /locus_tag="PKY63_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00463.1"
                     /translation="MKKLLLPLLIIIVLSSSCSKWGWVTLQYPNPPQLTLSDNIKTIA
                     IVNRSLVKQDDKNKNILETVASAEVAGSDKLASDECIKAVFDRMNGWSDISI"
     gene            570..2426
                     /locus_tag="PKY63_07350"
     CDS             570..2426
                     /locus_tag="PKY63_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPF00464.1"
                     /translation="MTAQNNRKVVNKDDVVIKFVGDSGDGMQLLGTIFSETIALAGED
                     LSTFPDFPAEIRAPRNTVAGVSGFQVHFGFKKVLTPGDQCDVLVAFNPASLKANLKNI
                     KKGGTIITDEDSFEEKVLSPLGYTTNPLFDGTLDGYNVIHPPITSLAKGSLKDFGVDN
                     KTAEKTKNMFVLGMLFNMFSLDLKATDDYLQKRFKKKPELIKINEIVIKNGYEFANTI
                     EAIPSIVVPKTQLKPGKYRNIHGNEATAWGLMAAAEKAGLQLFLGSYPITPATEILME
                     LAKHRELGVKTFQAEDEIAGICSAIGASFAGSLACTSTSGPGLSLKSEALGLAVMTEL
                     PLVVVNVQRGGPSTGLPTKSEQADLLQALYGRNGEGPVIIIAASSPADCFYTSYESAK
                     YSLEHMTPAICLTDGYIGFGSEVIEVPKMAELPTITPRKAKPRKDGDGRFLPYERDPE
                     SLAREWAVPGTEGLRHRIGGLEKSNIYGEVTTDPINHELMVKLRAEKVQRIANHLPMQ
                     EFTGPADADLLVVSWGGTKGQVQAGVEEARAQGMKIAHTHFRYISPLPKNTRDMLASF
                     KKIVVCELNMGQFAAYLRTNFPEIPYLQYNKVQGQPFTASELVENFSQILKA"
     gene            2431..3465
                     /locus_tag="PKY63_07355"
     CDS             2431..3465
                     /locus_tag="PKY63_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPF00465.1"
                     /translation="MMGVIRNSIEKSPVPLTKADFVSDQMVKWCPGCGDHSILNAVSK
                     VFPEIGYKKENYVIVSGIGCSSRFPYYVNSYGFHGIHGRAHAIASGVKIANPGLSVWI
                     PTGDGDSLAIGGNHFIHLIRRNIDVNVMLFNNQIYGLTKGQYSPTSPLGAQTKTSPYG
                     TIEHPFSVGELVIGAQGTFFARAIDTNPPMMTEIMFEAAKHDGTSIVEILQNCVIFND
                     KAHEYITSKENRDNMQLHLKHGEKMIFGKEKNKGIRLNDRSLEVVTIGENGISEEDIL
                     IHDMHNPDPGIHLMLAKLSSPIAFGVIRAAAAPTYDDLMEEQIAQVKAGSNIKCMDDL
                     FNSGETWEIK"
     gene            3585..4262
                     /locus_tag="PKY63_07360"
     CDS             3585..4262
                     /locus_tag="PKY63_07360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPF00466.1"
                     /translation="MNLLLLAITPIFILAFYLYYRDKYEKEPIGMLIKALIAGAIIVI
                     PILFVEIFITKYVDKYSGSTGAFLNAFAVAGFTEEIFKWLAVYLLFFRSRHFNERFDG
                     IVYAGIVSLGFALVENIMYVIGNDSASVGYMRAFTAVPAHMLFGVVMGYRFGLAKFLP
                     AAKSWQLFMAFFMPFLLHGTYDFLLMLEKTWALILFFPFVIFMWYFGLVRMKRHTLNS
                     AFNNTKP"
     gene            complement(4252..4851)
                     /gene="yihA"
                     /locus_tag="PKY63_07365"
     CDS             complement(4252..4851)
                     /gene="yihA"
                     /locus_tag="PKY63_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPF00467.1"
                     /translation="MIAKYISGCTTWTKCPPAHLPEFAFTGRSNVGKSSLINAMTGIN
                     KLAKISSTPGKTQIINHFLIDNNWYLADLPGYGYARISKKTREKWQPMMREYLNRREN
                     LMTTFVLIDSRLEPQKIDLEFIDWLGENSIPLALVFTKADKCSKNELKRNVDSVLKKI
                     SENWEELPAHVITSSETGAGIEDLLQFIDGVNRLWVPMA"
     gene            complement(4866..5801)
                     /locus_tag="PKY63_07370"
     CDS             complement(4866..5801)
                     /locus_tag="PKY63_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF00468.1"
                     /translation="MKPLIDKVSREEILRELSPERFVRYTNFGHNEIYIFRGDDAPNL
                     MLEIGRLREIAFREAGGGTGEPVDIDEFDKGSNAYFQLIVWNPGEQEIIGGYRYIKLK
                     DTHPDEHGYYHLATTHMFRFSEKFMDEYFSDTIELGRSFVQPQYQPSRDSRKGIFSLD
                     NLWDGLGGLIIMHPDIKYFYGKVTMYLKYDQFARDVILSFLMKYFPDRENLVWAYVPR
                     PIRTTDDVVKNILNGKTYEEDKMLLMRFVRSRGMNFPPLVNAYMNLTSSMRTFGSSLN
                     DTFGDVEEIGILVTIADIYESKKHRHLASFNKGRD"
     gene            complement(5843..6703)
                     /locus_tag="PKY63_07375"
     CDS             complement(5843..6703)
                     /locus_tag="PKY63_07375"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPF00469.1"
                     /translation="MEVQSNRIENGSLVITERTIDVKQLIREKNARLERLLPGFVIRY
                     LRRILHEKDINATLFQNRDKHGVEMVSAVLSDFGATIIVRGIENLDGENRFIVVANHP
                     LGGLDGLALIQTVGRVKPPIRFPVNDFLLYLPNMRELFIPINKVGSQSSAGVKALEEA
                     FLSNDNILYFPSGLASRKINGKIIDLEWKKTFVQKARQHQRNVIPVHVSGQNSRRFYR
                     IANWRKRLGIKVNIEMLYLADEMYKQKGQTITITFGKPLQYTMFDKSKKDTEWAEYVK
                     QIVYNLPQND"
     gene            6941..7411
                     /locus_tag="PKY63_07380"
     CDS             6941..7411
                     /locus_tag="PKY63_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00470.1"
                     /translation="MAAFIGVYDLKADSKGRFVFPASLRKQLAAEASEAFVMKRSVFY
                     KCIELFPQSTWARETEGIARLNRFVKKNNDFIRLFMSGSRIVNIDDGGRLLIPKDLMI
                     FSGISRDIVLASAIDRLEIWSKAEYEAFIAGGSADFPTLAEDVMGNAASSPEEK"
     gene            7408..8304
                     /gene="rsmH"
                     /locus_tag="PKY63_07385"
     CDS             7408..8304
                     /gene="rsmH"
                     /locus_tag="PKY63_07385"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPF00471.1"
                     /translation="MTHHESVLLQESVDYLAVKNDGRYADATFGAGGHSLLIAKQLKT
                     GHVYGFDQDEDAVNGFHGHPNITVIRSNFRFIHNFLRYYEALPVDGILADLGISSHHI
                     ETPQRGFSFRFDAPLDMRMNTASDFNAQQIVAQYSVEDLTRVFRNYGEVDQAWKIANA
                     IEKARNEKTVQTTFQLAEIISKFARAGRENSLMAQVFQALRIEVNHEMEALQEFLDQS
                     YKCLRPGGRLVIIAYHSLEDRMVKAFFKTQKSNDSFAQNIMGQKDLMWKDISRGAITP
                     SNEELTKNPRSRSARLRVAEKI"
     gene            8301..8705
                     /locus_tag="PKY63_07390"
     CDS             8301..8705
                     /locus_tag="PKY63_07390"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPF00472.1"
                     /translation="MKEKKNTTNDLREKDEPKTAAKKDQRKKRFAPGSILTGSFLEKE
                     GLIRQLPFILYIFLLLLFYIANAYYGEKTVYRIEQYKSEMIELRSEYISSKTRLMTGT
                     NIGKLSEKLKPENIFPSHTPPGKIFVQKQEDE"
     gene            8698..10827
                     /locus_tag="PKY63_07395"
     CDS             8698..10827
                     /locus_tag="PKY63_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPF00473.1"
                     /translation="MSKKETSVNKTVFSRAFLIYALSGIFTIAIIYKLIQIQFVEGEE
                     LRKKAQNLSVKYTDIKARRGNILADDGSLLATSVPRFDLFVDLSPKTIDEKLFKDSLA
                     TLSKHLSEFFKDKTAEEYKTLLKNERAQNNRYLRIARNLDFFDMKEVASFPIFNAGRY
                     RGGLIIVQNDRREMPFGFLAKRTIGYIRDNYPVGIEGAYEKELSGTDGKRLMKRVSGQ
                     NWIPVDDKADILPQDGKDVVTTIDIHLQDVAETALETHLEKHEAHHGCAVLMEVETGE
                     IKAIANLQRMPDGNYAESYNYAIGEAFEPGSSFKLMSMLVALEDGKINLDDKVNTGDG
                     KFRYGKKEVMTDSHKGGFGTISIRQAFELSSNIGISGIIVKAYGANPQKFIDGLYKLG
                     INKPLGIELKGEGIPYIKNRSDKTWSATSLPWMSIGYELRMTPMQILAIYNAVANDGA
                     LMKPMFVKELQYAGQTVKKFEPVVLNKSIAGNKTIKLAQELLEGVVLRGTATNLKNAV
                     FPIAGKTATAQIASAGGYKNNEGPEYFASFVGYFPADNPRYSCIVVVNKPKKGQYYGG
                     SVAAPVFLEIAEKIYATRAEMRKQDTSNTQIKNDPGCIYAAYNKAIDMYAELGINLKQ
                     KVEAENDDLVFINLSSDSLKYNVSNPNKSRLINLRGMAATDAIAYLESKGAVVSLSGK
                     GWVISQSPQAGTPVRQGMKVKLTLGPR"
     gene            10827..12278
                     /locus_tag="PKY63_07400"
     CDS             10827..12278
                     /locus_tag="PKY63_07400"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPF00474.1"
                     /translation="MKLIDILKNTDYRVVQGNNAIETTGMTASSREVKKGFVFFALKG
                     VKADGHQFIPDAIKAGAAAIVCLNIPEVIPENITVVVTKNNNETYAQAAANWFGNPSS
                     ELHLVGVTGTNGKTTTATLLFELFNKSGIKSGLISTIRYIVPGKEYPATHTTPDALVL
                     NRMLREMADADCEYCFMEVSSHAVVQGRIHGLKFKGGIFTNLTHDHLDYHGTFAEYLK
                     AKQLFFDHLPQSAFALYNDDDANGKVMVQHSKAKCFSYALSRPADFKTKIIHNAITGL
                     GLSINGTEAWFKLVGRFNAYNLTAIFATATLCGMQKEEILVTLSSLGEVEGRFNVIRS
                     ESGITGVVDYAHTPDALKNVLSTIEEVNEGSGDIITVAGAGGDRDKSKRPLMGQMMAR
                     YSQKVIITSDNPRTEDPDTIINEIRNGIDASEMKKVICITDRREAIKTACLLAKKGDI
                     ILVAGKGHETYQEINGVRNHFNDAEQLNEFLKI"
     gene            12283..13548
                     /gene="mraY"
                     /locus_tag="PKY63_07405"
     CDS             12283..13548
                     /gene="mraY"
                     /locus_tag="PKY63_07405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPF00475.1"
                     /translation="MLYYLFDFLDKQYDFPGAGVFQYLSFRAALAAALSLLISMVFGK
                     RIINFLQKNQIGEDIRDLGLEGQMQKKGTPTMGGIIILAAILIPTLLVAKLHNIYIII
                     ILLATVWLGTLGFIDDYIKVFRKNKAGLHGKFKILGQLGLGLIVGFVMYFNENIVIRE
                     KIDTSQHLTEADVLNKDCKKEAADFFSTEEIKSLKTTIPFVKDNEFDYGVLTSWMGDA
                     ASKWGWLIFIPIIMFIIVAVSNGANLTDGMDGLAAGTSAVVGIVLGLLAWISGNFIFA
                     DYLNVMYIPNSGELTIYISAFVGAMIGFLWYNSYPAQVFMGDTGSLTIGGIIAVFAIM
                     IRKELLIPILCFVFVIESLSVIIQTTVFKYRKKRFGYEYASAHRVFLMSPIHHHYHKK
                     GLNEVKIVNRFIIVSIFAAILTIVTLKLR"
     gene            13563..14111
                     /locus_tag="PKY63_07410"
     CDS             13563..14111
                     /locus_tag="PKY63_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPF00476.1"
                     /translation="MKIETLALQGKPNLNNTMAASVAATMFEFRKDTLKKSFQTFQKT
                     EHRLEFVVKVHGISFWNDSKATNVNSTWYALESMKTPIIWIAGGTDKGNEYDCLFELV
                     KNKVKALICLGADNSKLIETFSPYVEVIADTHSMEEAVNAAYQLGNSGDVVLLSPACA
                     SFDLFENYEDRGNQFKNAIREL"
     gene            14114..15265
                     /locus_tag="PKY63_07415"
     CDS             14114..15265
                     /locus_tag="PKY63_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPF00477.1"
                     /translation="MKFFSQHIKGDKVVWIIVLLLSLFSILSVYSSTGSLAFRYQGGN
                     TEYYLLKHVFTIITGLFIMYLIHNMKYTLFSRMAQLLFILSVPLLLYTLIKGTNVNEA
                     ARWVTVLGISFQSSDVAKLAVILFLARALALRQDKIDDLKSSFIPIIWPVGLVTILIF
                     PANLSTAVLVFGTALIIMYIGRIPVKYIAAAIGALVLVGSLAVLIIMQSPGKGRTGTW
                     ANRIESYFSDEETEGDYQALQSKIAIANGGITGKLPGNSTQRNFLPHPYSDFIYALIV
                     EEWGLFGGIFVLLLYLILLYRGIRIATRSPGSFGAFLSIGLTIMIVFQALINMGVAVN
                     LLPVTGQPLPLVSMGGTSIWFTCISLGIILSVSRNKEEEKEIANENTKA"
     gene            15246..16370
                     /gene="murG"
                     /locus_tag="PKY63_07420"
     CDS             15246..16370
                     /gene="murG"
                     /locus_tag="PKY63_07420"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPF00478.1"
                     /translation="MKTQRPKFIFSAGGTGGHIYPALATADALRDLLPEAEILFIGAE
                     NRMEMEKVPHHGYPIIGLPIQGIQRKLTLKNLAVPFKVIASLIKARKILKQSKPDVVA
                     GFGGYASGPVLRKAASMGIPTLLQEQNSFPGLTNRILSRKADSICVAYPGMDQFFCQE
                     KITLTGNPIRSEIFSQSISPEDGRKFFGLDTKKTTILAIGGSLGARTINESLCSLIPE
                     ILEKDYQVIWQTGKNYSYKSCNKFDAEKNKNIFVQEFIYEMPQAYAAADVIISRAGAI
                     AVSEIAAVAKPVIFIPSPNVTDDHQTKNAMVFVNHKAALIVKDNEARESLSKMLFTLI
                     DNHNLRSEMCENLKKLAMPDAAKSIAKKIIELREKKLNGH"
     gene            16360..17721
                     /gene="murC"
                     /locus_tag="PKY63_07425"
     CDS             16360..17721
                     /gene="murC"
                     /locus_tag="PKY63_07425"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPF00479.1"
                     /translation="MDIKKIHIAYFLGIGGIGMSALARYFNMNGVQVSGYDKTSTPLT
                     KALEAEGIAVHYAADENAIPDNADIVVYTPAVPADFAEWEKIRQLNLPVIKRSAMLAE
                     IINSMKSIAVAGTHGKTTISGMIAHSLEHIGKPFLGFVGGVLCGYNKNIFLHENAEWA
                     VAEADEYDRSFLALHPRIAIINAIDADHLDIYGQIEELHKSFSDFAAQTNSEGHVLLY
                     DRADKTKLKLPENTILYGFENTEGYSARNIHVCTNRFVFDLYLNNQLLEGAIHLPAAG
                     RHNIQNATAAFAAMHLAGCETTKIVQALESYPGVKRRLELISKNSKIIYYDDYAHHPA
                     EIDALINTIRELYPGKTITGIFQPHLFSRTRDFGKDFGQSLAKLDVPVITDIYPAREK
                     PIPGIDANWLLELIPNVHKKYIPYNQLPEIVNTCDEGILLTIGAGDIDMHISTIKNRI
                     EQS"
     gene            17718..18551
                     /locus_tag="PKY63_07430"
     CDS             17718..18551
                     /locus_tag="PKY63_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00480.1"
                     /translation="MKPIYKILILSLVIIVVLVLLKLSNDNFKKQKISQVNITINTQS
                     GPELVTEEEVRTLISQGYDSLTKKTVEEIDLVWIENITRTNPYVLTADAFINIDASLT
                     VDILQRKPILRVFNQKNESFFIDAEGKMLPLNRGASPGLIVAAGDIQERFLPSQKYQH
                     FDSVSFDQTKVLSTMHKLYLIASEISKNEFLSRLITQIYVNDNTHYELIPVIGNQNII
                     LGDVDGLKIKLKNLHHFYKKGYNTINFSKYKSFDIRFHNQVVGIKETYEINEIKKENE
                     I"
     gene            18548..19822
                     /gene="ftsA"
                     /locus_tag="PKY63_07435"
     CDS             18548..19822
                     /gene="ftsA"
                     /locus_tag="PKY63_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPF00481.1"
                     /translation="MNENQEIMVGLDIGTTKIAMIVGYQNEHGKLEVLGHGKTESLGV
                     KRGLVANIDKTVEAIKKVVEITEAKSNVNIRTVNVGIAGQHIKSLQHRGSIIRENTDN
                     EIGQEDVERLIENMYKLVMPPGEEIIHVIPQEYIVDGEQGIKDPVGMAGVQLEANFHI
                     ITGQILAARNIEKCITRADISMSDMVLEPIASSYSVLSEEEKEAGVALVDIGGGTTDI
                     AIFQDGIIRHSAVIPLGGDIVTEDVKEGCSILKSYAETLKVKHGSALANEVKDEIYIS
                     IPGLKGRPHKEISMKNLARIIQSRMEEIIEFVNYEIKNSGYDRKLIAGIVLTGGGAQL
                     KDIAQLCQFITGHETRIGYPSEHLSANVDKELLLPMYATCIGLVLNGIENSLASQSNS
                     KKTIPNQGKRERGGFIKKIFRGGMNFFDENIS"
     gene            19926..21506
                     /gene="ftsZ"
                     /locus_tag="PKY63_07440"
     CDS             19926..21506
                     /gene="ftsZ"
                     /locus_tag="PKY63_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPF00482.1"
                     /translation="MSETENSIHIQFEDTQSLPTIIKVFGVGGGGSNAVNHMFKQGIN
                     GVDFVICNTDVQSLGTSPVPVKLQIGAGLTEGRGAGNSPEIGRNAALENADEIRELLS
                     QNTKMVFITAGMGGGTGTGAAPVIAQVAKELDILTVGIVTTPFGFEGGKRKNQASQGI
                     DEMRKYVDSLIIINNDKVREHYGNLKLSEAFCKADDILTVAAKGISELITIPGYMNVD
                     FEDVKAIMKNSGIAIMGTGIAEGEDRAQKAVELALNSPLLDDNEIYGARNVLLFISSG
                     EDEISMDEVSDISDYIQNAAGSTAEVIWGTGTDLSLGNSISVTVVATNFNKHSSPVKS
                     IYEEPQVINLQPEVKQEMPLAEPETEMKVVVKPVEEKPVINLYQPPIIPATPIVDQPV
                     EKITIQLEQEQEPEQNNNPMNLFPSENETKTSPQPMIHDVLPAEKNQEEPLQTPIRPE
                     LQDERMQNLKNFNFTNPKATNLDEIEQVPAYARRGVNLAPENPSDEKIVSRFTLGENG
                     SNKPEIRENNSFLHDNVD"
BASE COUNT         6813 a         4908 c         4472 g         5669 t
ORIGIN      
        1 aatactgatg tccgaccagc cgttcatgcg gtcgaatact gccttgatgc attcatctga
       61 agccagtttg tcggagccgg ccacttcggc cgaagccacc gtctcgagga tgtttttatt
      121 cttgtcgtct tgtttcacca gagaccggtt tacaatggcg atggttttaa tattgtcgga
      181 aagtgtaagc tgaggcgggt tcggatattg cagggtgacc cagccccatt tgctgcaact
      241 gctcgataaa acaatgataa tcagcagcgg gagtaataat ttcttcataa aaatccggat
      301 ttggtggttt ttagaccgcg ccgcaaagca aaagttgtac cgagggctga tttaaacatt
      361 caggtaattg catatgattg ataaacaagt tgtaaataat cgacctaaca tcggatatta
      421 aaaaggattg actcttcaaa gcaagtgtct ttgcggttta cagaaataaa acattaaaaa
      481 tcaacattaa tattgacttt tttaagaaca tctttttcag cctgtatagg aaattcgata
      541 tacctttgca caattttaat aaccaacata tgactgcaca aaataaccga aaagtggtta
      601 acaaagatga tgttgtcatc aagtttgtcg gagactccgg cgacgggatg cagcttctcg
      661 gaaccatttt ctctgaaacc atcgctttag ctggtgaaga tttgtcaaca tttcccgatt
      721 tccctgccga aatcagggct ccgcgcaaca ctgttgccgg cgtatcagga tttcaggtac
      781 atttcggttt caaaaaagta cttactccgg gggatcaatg tgatgttcta gttgctttta
      841 atccggcatc gctgaaggcc aacctgaaaa acattaaaaa aggtggcacc atcattaccg
      901 atgaagacag cttcgaggaa aaagtactca gtccgctggg atacacaacc aacccattat
      961 ttgatggcac cctcgatgga tacaatgtta ttcacccacc catcacttcg ctggccaaag
     1021 gaagtctcaa agattttggg gttgacaaca aaacagctga aaaaaccaaa aacatgtttg
     1081 tacttggcat gctgttcaat atgttcagtc tcgatttgaa agcaaccgat gattatctgc
     1141 agaagcgctt caagaaaaaa ccggaactga ttaaaattaa tgaaatagtc attaaaaacg
     1201 gttatgaatt tgccaacacc atcgaggcaa ttccaagcat cgttgttccc aaaacacagt
     1261 tgaaacccgg caaataccgc aacattcacg gaaacgaagc caccgcctgg ggacttatgg
     1321 ctgctgccga aaaagcagga ctgcaattat tccttggctc atatcctatc actccggcta
     1381 ccgaaattct gatggaactg gccaaacacc gcgaactggg tgtaaaaact tttcaggccg
     1441 aagacgaaat tgcaggtatc tgttccgcaa tcggagccag tttcgcaggt tcactcgcct
     1501 gtacctccac ttcagggcct ggactctcac tcaaatcaga agctcttggg ctcgctgtta
     1561 tgacagaatt gccattggtc gttgtaaatg ttcagcgtgg cggtccttca accggcttgc
     1621 caaccaaaag cgaacaggcc gaccttttac aggcactcta tggccgcaac ggcgaaggcc
     1681 cggtaattat cattgccgct tcgtcaccag ccgactgctt ctatacatcg tacgaatcag
     1741 ccaaatattc acttgaacac atgacgccgg cgatttgcct caccgatggt tatatcggtt
     1801 ttggatccga agttattgaa gttcccaaaa tggctgagct tcccaccata actccacgca
     1861 aagccaaacc acgaaaagac ggtgatggca gatttttacc ttatgaacgt gatcccgaat
     1921 cactggcacg tgaatgggcc gttccgggaa ccgagggact gcgccatcgt attggcggtc
     1981 ttgaaaaatc aaatatttat ggcgaagtta ccaccgaccc gatcaatcat gaactcatgg
     2041 tgaaactacg cgcagaaaaa gttcagcgta ttgccaatca tcttccaatg caggaattca
     2101 ccggtcccgc agatgccgat ctgcttgtgg ttagctgggg tggtaccaaa ggccaggtgc
     2161 aggcaggtgt tgaagaagca agagctcagg gaatgaaaat agctcacact cacttccgct
     2221 atatcagccc gctaccgaaa aatacccgcg acatgcttgc ttcattcaaa aaaatcgtgg
     2281 tatgtgaact caatatggga cagtttgccg cttatttgcg aaccaacttc cccgaaattc
     2341 cttacctgca atacaacaaa gtgcagggtc agccatttac ggcaagtgaa ctggtcgaaa
     2401 atttcagtca aatcttaaaa gcttaatatt atgatgggcg ttatcagaaa cagcatagaa
     2461 aaatcaccgg ttcctttgac aaaagcagat tttgtcagcg accaaatggt gaaatggtgc
     2521 cccggttgtg gtgaccacag catcctcaac gcagtatcga aagttttccc ggaaatcggt
     2581 tataaaaagg aaaattacgt tattgtttcc ggaatcggtt gttcatcgcg ttttccgtat
     2641 tacgtgaatt cctatggttt tcacggcatt cacggacgtg cacatgccat tgcatcgggt
     2701 gtaaaaattg ccaatccggg cctcagcgtc tggattccaa ccggcgacgg tgactcactc
     2761 gccatcggcg gaaaccattt cattcacctc atacgacgaa acatcgacgt aaacgtgatg
     2821 ctcttcaata atcaaattta tggcctcacc aaaggacagt actcacctac aagcccgctg
     2881 ggagcacaaa ccaaaacatc gccatacgga accattgagc atcccttctc cgtaggtgaa
     2941 cttgtcatcg gtgcccaggg aactttcttt gcacgtgcca ttgacaccaa cccaccgatg
     3001 atgaccgaaa tcatgttcga agctgccaag cacgacggta cttcgattgt tgaaatcctc
     3061 caaaactgcg ttattttcaa cgacaaagca cacgaatata tcacttcgaa agaaaaccgc
     3121 gacaatatgc aactgcattt gaaacacggt gaaaaaatga ttttcggcaa agaaaaaaac
     3181 aaaggcattc gactcaatga ccgaagtctt gaagttgtta ccattggtga aaacggcatt
     3241 tcggaagaag acatcctgat tcacgacatg cacaaccccg atccgggaat tcatctcatg
     3301 ttggccaaac tgtcgagccc gattgccttt ggcgtaatcc gcgctgctgc tgctcctacc
     3361 tatgatgatt tgatggaaga gcaaattgct caagtcaaag cagggagcaa tattaaatgc
     3421 atggacgatc tgttcaacag cggcgaaacc tgggaaatta aataattatt cccaaacaca
     3481 tttcttaaaa aaccggcttt tcaagccggt ttttttattt caggtttgtc ttatcaatat
     3541 tatacttcac atttccaatc attattatct ttacacaaaa aaagatgaat ttattgctcc
     3601 tggccattac cccgattttc atccttgcat tttatcttta ttacagagac aaatacgaaa
     3661 aggaacccat cggaatgctc atcaaagctc tgattgctgg agctataatt gtcattccca
     3721 ttctgtttgt tgagatattc atcacaaaat atgtggacaa atattccggc tccaccggtg
     3781 catttctgaa tgcctttgcc gtggcaggtt tcactgagga aattttcaaa tggctggcgg
     3841 tttatctgtt gtttttccgt tcaagacatt tcaacgagcg tttcgacggt attgtgtatg
     3901 cgggcattgt atcgcttggt tttgcattgg tcgaaaacat catgtacgtg attggaaatg
     3961 actcggcatc ggttggctac atgcgcgcat tcacagccgt tccggcacac atgctgtttg
     4021 gcgttgtgat gggatatcgt ttcggacttg caaaattttt accagctgca aaatcgtggc
     4081 agctattcat ggctttcttc atgccgtttc tgcttcacgg aacctacgat tttctgctca
     4141 tgctcgaaaa aacatgggcg ctgatattgt tcttcccatt cgtcattttc atgtggtatt
     4201 tcggccttgt gcgcatgaag cgtcacacgc tcaactctgc attcaacaat actaagccat
     4261 aggaacccac agacggttga ccccatcaat aaattggagc agatcttcaa ttccggcacc
     4321 ggtttcggat gaagtaatca catgagccgg cagttcttcc cagttttcgg atattttctt
     4381 cagcacagaa tcaacattcc gcttgagttc attctttgaa catttgtcgg ctttggtaaa
     4441 aacaagtgcc agcgggatac tattctcgcc aagccaatca atgaattcca gatcgatttt
     4501 ttgcggttca agacgagaat caatcagcac aaatgtagtc atcagatttt cgcgccggtt
     4561 cagatattca cgcatcatgg gctgccattt ttcccgtgtt tttttcgaaa tacgtgcata
     4621 accataaccc ggcaaatcgg caagatacca attgttgtca atcaaaaaat gattaataat
     4681 ctgtgttttc cctggtgtag agctgatttt ggccagctta ttaattccag tcattgcatt
     4741 gataagcgac gactttccaa cattggaacg acctgtaaaa gcaaattcag gcaaatgcgc
     4801 cggcgggcat ttggtccacg tggtgcaacc tgaaatatat ttagcaatca taaaaaaaga
     4861 aggaattaat cacgcccctt attaaaagac gccagatgcc ggtgtttctt tgattcataa
     4921 atgtcggcaa ttgtaacaag aataccgatt tcctctacat caccaaaagt atcattgagc
     4981 gaagaaccaa aagtgcgcat tgaacttgta agattcatat atgcattgac caatggagga
     5041 aaattcatgc cgcgactacg cacaaaacgc atcaacaaca ttttgtcttc ttcgtatgtt
     5101 ttcccattga gaatattttt aacaacatca tcagtggtac gaataggacg tggaacatag
     5161 gcccagacaa gattttcacg atcagggaaa tatttcatca gaaacgacag aatcacatcg
     5221 cgcgcaaact ggtcgtattt cagatacatg gttaccttgc cataaaaata ttttatatca
     5281 ggatgcatga taataaggcc accaagccca tcccagagat tatcgagtga aaaaatgcct
     5341 ttgcggctgt cgcgtgatgg ctgatattgc ggctgaacaa atgaacgacc aagctcaatg
     5401 gtatcactga aatattcgtc catgaatttt tcagaaaacc gaaacatatg tgtggttgcc
     5461 agatgataat aaccatgctc atcgggatgc gtatctttta gtttgatgta gcgataaccc
     5521 ccaatgattt cctgttctcc cggattccac acaatcaact gaaaataggc attcgaaccc
     5581 ttatcaaact catcaatatc gacaggttct cccgtgccac cacctgcttc gcggaatgca
     5641 atttcacgca ggcggccaat ttcaagcatg agattaggcg catcatcacc acgaaaaata
     5701 taaatctcat tatgaccgaa atttgtataa cggacaaagc gctcaggcga tagctccctg
     5761 aggatttctt cacgtgaaac tttgtctatc agcggtttca tattaatccg taatatacca
     5821 aaaacaaaat aagggtgtaa aattaatcat tttgcgggag attgtaaaca atttgtttca
     5881 catattctgc ccattcagta tcttttttgc ttttgtcaaa catcgtatat tgaagtggtt
     5941 tgccaaaggt aatggtaatg gtttgcccct tttgtttata catttcatca gccagataaa
     6001 gcatttcaat gttgacttta atgcccaacc gtttgcgcca atttgcaatg cgataaaaac
     6061 gccttgaatt ttgcccgctt acatgaaccg gaatcacatt gcgctggtgc tggcgcgctt
     6121 tctgcacaaa tgtctttttc cattcaagat caataatttt tccattgatt tttcgtgagg
     6181 ccaaacccga cggaaaatac agaatgttat catttgacaa aaaggcctct tcaagcgctt
     6241 tcactccagc cgaagactga ctgccaactt tattgatggg gataaacaat tcacgcatat
     6301 tgggcagata caataaaaaa tcattcaccg gaaaccggat aggtggcttc acacggccaa
     6361 ctgtttgaat caatgccaaa ccatccaacc cccccaacgg atgattggct accactataa
     6421 aacgattttc accatccaaa ttctcgatgc ccctcacaat gatagtagct ccaaagtcgg
     6481 acaaaaccgc actaaccatt tcaacaccat gcttatcgcg attttgaaac aaagtggcat
     6541 taatatcttt ttcatgcaaa atccgccgca gataacgaat aacaaaaccg ggcaaaagcc
     6601 gctccaaacg agcatttttt tcgcggataa gctgttttac atcaatggtc ctttctgtta
     6661 ttaccagcga cccattttca attcggttac tctgaacttc catatctccc tttttgctgt
     6721 gcaaatgtaa gaacttccca ccaaatcagc gtttgagtga gttattcacc attttccgaa
     6781 cttccccact ttttatcatt ttataagcat ctgtctgtca atattttata atttaatcgt
     6841 aacctttttg cttcaaataa gtaaaaagtt atcaacaaag tggggaaaaa tggctttttg
     6901 tggggaagaa ttctttactt ttacacttta aaatcctgaa atggcagcgt ttatcggagt
     6961 atatgattta aaagcagatt cgaaggggcg gttcgtattt ccggcctccc tgcgaaagca
     7021 gcttgccgcc gaggcctccg aagcgtttgt catgaagaga agtgtgtttt acaagtgtat
     7081 tgagcttttc ccacaatcaa cctgggccag ggaaaccgaa ggaatagccc ggctgaaccg
     7141 ctttgtgaaa aaaaacaatg attttatccg tttgtttatg tccggctcgc gcattgtcaa
     7201 tatcgatgat ggcggccgct tgctgattcc aaaagatctt atgattttca gcggcatttc
     7261 aagggatatt gttcttgcat cggccatcga ccggctcgaa atctggtcga aagccgaata
     7321 cgaagcgttc atcgccggcg gatccgccga ctttccgact ctggcagaag atgttatggg
     7381 aaatgctgcc agcagcccgg aagaaaaatg acgcatcacg aaagtgtttt acttcaagaa
     7441 agtgttgatt acctcgccgt gaaaaacgac ggacggtacg ccgatgccac ttttggtgcc
     7501 ggcggtcatt ctttgctgat tgctaaacaa ctcaagaccg gccatgtata tggattcgat
     7561 caggatgaag atgctgtaaa tggatttcat gggcacccca acatcactgt tatccgttcc
     7621 aacttcagat tcatccacaa tttcctgcgt tattacgaag cgctgcccgt agatggcatt
     7681 ttggccgatc tcggaatatc aagccaccac atcgaaacac ctcaacgcgg attttcattc
     7741 cgctttgatg ctccgctcga catgcgcatg aacactgcat cggatttcaa tgcacaacaa
     7801 attgttgccc aatacagcgt tgaagacctt actcgcgttt tcagaaatta tggcgaagtt
     7861 gatcaggcct ggaaaatagc caacgccatt gaaaaagcac gcaacgaaaa aacagtgcag
     7921 actactttcc aactggccga aatcatcagc aaatttgcaa gagccggacg cgaaaacagt
     7981 ttgatggcac aggtgtttca ggcgctacgc atcgaagtga atcacgaaat ggaagctctc
     8041 caggagtttt tagatcaaag ctacaaatgc ctccgtcctg gtggaagatt agtgattatt
     8101 gcatatcatt cgctcgaaga ccgcatggtt aaagcatttt ttaaaacaca aaaatcaaac
     8161 gattcttttg cccagaatat tatgggacaa aaagacctga tgtggaaaga catttcgcgc
     8221 ggagctatca cgccatccaa tgaagaattg accaaaaacc cacgcagcag aagtgccaga
     8281 ctgagagttg cagaaaaaat atgaaagaaa agaaaaacac gacaaatgat ctgagggaaa
     8341 aggatgaacc caaaaccgct gcaaaaaaag atcagcgcaa aaaaaggttt gctcccggat
     8401 ccatcctgac cggtagtttt cttgaaaaag aaggccttat caggcaactc cctttcattc
     8461 tgtacatttt tctgctgttg cttttctata tcgccaatgc ttattacggc gaaaaaacag
     8521 tatatcgcat tgagcaatac aaatcggaaa tgattgaact ccgaagcgaa tatatttctt
     8581 cgaaaacccg cctgatgacc ggcaccaaca taggcaaact aagcgaaaaa ctaaaaccgg
     8641 aaaacatttt tccatcacat actcctccgg gaaaaatatt tgttcaaaaa caggaagatg
     8701 agtaaaaaag agaccagtgt aaataaaacg gttttcagca gggcatttct catttatgct
     8761 ttgtcgggga tttttaccat cgccatcatc tacaaactga ttcagattca atttgtcgaa
     8821 ggagaagagc ttcggaaaaa agctcagaat ctttccgtca aatataccga catcaaagca
     8881 cggcgcggaa acattcttgc cgatgatggc agtctccttg caacatcggt tcctcgcttc
     8941 gacctgtttg ttgatttaag tccgaaaacc attgatgaaa agcttttcaa ggacagcctt
     9001 gcaacactga gcaaacatct gagtgaattt ttcaaagaca aaacagccga agaatacaaa
     9061 acactgttga aaaatgaaag agcgcaaaac aaccgctatt tacgaattgc aagaaatctc
     9121 gactttttcg atatgaagga agtcgcctct ttcccgattt tcaatgcagg aagatacaga
     9181 ggcggactca ttatagttca aaacgaccga cgcgaaatgc cttttggttt tctggcaaaa
     9241 agaaccattg gctacatacg cgataattat ccggttggca ttgaaggagc ttacgaaaaa
     9301 gaactcagtg gaactgatgg aaaacggctt atgaaacgcg tttcaggcca aaactggatt
     9361 ccagttgatg ataaagcgga tatacttccg caggatggaa aagatgttgt aactaccatt
     9421 gacatacacc tgcaggatgt tgcagaaaca gcacttgaaa ctcatctcga aaaacacgaa
     9481 gctcatcatg gttgcgccgt tctgatggaa gttgaaaccg gcgaaatcaa agccattgcc
     9541 aatcttcaaa gaatgcccga tggaaattat gccgaatcgt acaactatgc cattggagaa
     9601 gcttttgaac ccggatccag ctttaaactc atgtcaatgc tcgtagctct tgaagacggg
     9661 aaaatcaatc tcgacgacaa agtaaatacc ggagatggaa aattccgata tgggaaaaaa
     9721 gaagtcatga ccgattcaca taaaggtggc tttggaacca tttcaatccg gcaggccttt
     9781 gaactgtcat ccaatatcgg aatatcaggc atcattgtaa aagcatacgg agcaaatcct
     9841 caaaagttta tcgacggact gtacaaactg gggattaata agccactggg cattgaactc
     9901 aaaggagaag gcattccata catcaaaaac cgcagcgata aaacatggtc tgcaaccagt
     9961 ctgccatgga tgtctatcgg ctatgaactt cgcatgactc ccatgcagat tcttgccatt
    10021 tataatgcag tggcaaacga cggagctttg atgaagccca tgtttgtcaa agagctgcaa
    10081 tatgccggac aaacggtgaa aaaatttgaa cctgtagtat tgaataaatc aattgcaggg
    10141 aacaaaacca tcaaactggc acaggaacta ctcgaaggcg ttgtcctgcg aggaacggca
    10201 accaatctta aaaacgccgt tttccctatt gccggaaaaa cagcaacagc acaaatcgct
    10261 tcggcaggag gatacaaaaa caacgaggga cctgaatatt ttgcatcatt tgtcggttat
    10321 ttcccggccg ataatcctcg ctattcatgc attgttgttg tcaacaaacc aaaaaaagga
    10381 caatattacg gtggctctgt cgctgcaccg gtatttctcg aaattgcaga aaaaatttat
    10441 gcaacacgag ccgaaatgcg caaacaagac accagcaaca cacaaataaa aaatgatcct
    10501 ggctgcattt atgcagcata caacaaggcc attgacatgt acgcagaact ggggataaac
    10561 ttaaagcaaa aggtggaagc cgaaaacgac gatttggttt tcatcaatct atcttcagat
    10621 tctctgaaat acaatgtaag caatccaaat aaatcaagac ttataaacct gcgtggaatg
    10681 gccgctacag acgccatcgc ctatctcgaa agcaaaggag cagttgtttc cctcagcggt
    10741 aaaggatggg taatatcaca atctcctcag gcaggaacac ctgttcgtca gggcatgaaa
    10801 gtaaaactaa cactgggtcc gcgatgatga aactgattga catattaaaa aatacagatt
    10861 accgtgttgt tcagggaaac aacgccattg aaacgacagg catgactgct tcgagccgcg
    10921 aagtgaaaaa aggatttgtt ttctttgcgc tcaaaggcgt gaaagccgac ggacatcaat
    10981 tcatccctga tgcaataaag gcaggtgcag cagcgatagt atgcctgaac atcccggaag
    11041 ttattccgga aaacattaca gttgttgtaa caaaaaacaa caacgaaacc tatgcgcagg
    11101 cggcggccaa ctggttcggc aatccctcat cagaactgca tcttgtaggt gttaccggca
    11161 ccaatggaaa aaccacaact gcaacattgc tgtttgaatt gttcaataaa tccggtataa
    11221 aatcgggact gatttcaacc atccgctaca ttgttcccgg caaggaatat cctgctacac
    11281 acacaacccc cgatgcgctc gtcctgaacc gcatgctgcg cgaaatggcc gatgccgact
    11341 gcgaatattg tttcatggaa gtcagctccc acgccgtagt tcagggacga atccatggtt
    11401 tgaaatttaa gggcggaatt ttcacaaacc ttacacacga tcatctcgac tatcacggca
    11461 cttttgcaga atacctcaag gcaaagcaac tctttttcga tcacctgcca caatcagcat
    11521 ttgccttata caatgatgat gacgccaacg gcaaagtcat ggtgcaacac agcaaagcga
    11581 aatgtttcag ctatgcttta agtcgcccgg ccgacttcaa aacaaaaata attcacaatg
    11641 ccatcaccgg cctaggcctc agcatcaacg gtacagaagc atggttcaaa cttgtaggac
    11701 gtttcaacgc atataacctg acagccattt ttgccacggc aactctttgc ggaatgcaaa
    11761 aagaagagat acttgtaaca ttgagttcgc tcggtgaagt cgaaggaaga ttcaacgtaa
    11821 tacgctctga atcgggcatc acaggcgtag ttgactatgc tcacaccccc gatgcattga
    11881 aaaatgtttt atctaccatc gaagaagtca atgaaggcag cggcgatatc atcactgttg
    11941 caggagctgg cggagaccgc gataaaagca agcgtccgct gatgggtcag atgatggctc
    12001 gttatagtca gaaagtgatc attaccagcg acaatccgcg caccgaggat ccggacacca
    12061 tcatcaatga aatcagaaat ggaattgatg cctctgaaat gaaaaaagtt atttgcatca
    12121 ccgacaggcg cgaagccata aaaaccgcat gcttgcttgc aaaaaaaggc gacatcattc
    12181 tggtagcagg caaagggcac gaaacgtatc aggaaataaa cggcgtgcgc aatcacttta
    12241 atgatgccga acaattgaac gaatttttaa aaatataaag atatgctcta ctacttgttc
    12301 gattttcttg acaagcaata cgattttccc ggagcaggcg tgtttcagta tctgtcattc
    12361 cgtgccgctc tggctgcggc tctctctttg ttaatctcaa tggtatttgg aaaacgcatc
    12421 atcaatttcc tgcaaaaaaa tcaaattggt gaagacatac gcgaccttgg cctcgaagga
    12481 cagatgcaga aaaaaggaac tcccaccatg ggtggtatca ttattcttgc agccatcctt
    12541 attccgactt tgctcgttgc caaattgcat aacatctaca tcattatcat tctgcttgca
    12601 actgtttggt tgggaaccct tggctttatc gatgactaca tcaaagtatt ccgaaaaaac
    12661 aaagcaggat tgcacggaaa attcaaaatt ctcggccagc taggtttggg gctgatagtc
    12721 ggttttgtga tgtatttcaa tgaaaatatt gtaatccgtg aaaaaattga cacttcgcaa
    12781 cacctcaccg aagcagatgt actgaataaa gattgcaaaa aagaagcagc cgattttttc
    12841 agcacagaag aaatcaaatc actgaaaaca accatcccgt ttgtcaaaga caatgaattc
    12901 gactatggtg ttttaacaag ctggatggga gatgcagcat caaaatgggg ctggctgatt
    12961 tttataccga ttatcatgtt cattattgta gccgtttcga atggagccaa cctgaccgat
    13021 gggatggatg gactagcggc aggaacatcg gccgttgtag gcattgtact cgggcttctg
    13081 gcgtggattt caggcaactt catttttgcc gattatctca acgtaatgta tattccaaac
    13141 tccggagagc tgaccatata catcagtgct tttgtaggag ccatgattgg atttctctgg
    13201 tacaattcat atccggctca ggttttcatg ggcgatacag gaagtctcac cattggtgga
    13261 atcattgccg tttttgccat catgatacga aaagaactgc tcatccctat tctttgtttc
    13321 gtatttgtca ttgaatctct ttccgtcatc attcagacaa ctgttttcaa ataccgtaaa
    13381 aaaagatttg gttacgaata tgcatcagca catcgggtgt ttctgatgtc acccattcat
    13441 caccattatc ataaaaaagg actgaacgaa gtaaagattg tcaatcgctt catcatcgtc
    13501 agcatatttg ctgccattct caccattgta actctgaaac tgcgctaaac aaaacatatc
    13561 acatgaaaat cgaaacactg gcattacaag gcaaacccaa tctgaacaac acaatggctg
    13621 catcagttgc ggccaccatg tttgaattca gaaaagacac actgaaaaaa agttttcaaa
    13681 cattccagaa aacagaacac agacttgaat ttgtagttaa agtacacgga atttctttct
    13741 ggaacgatag caaagcaacc aatgtaaact ctacatggta tgcgctggaa agcatgaaaa
    13801 cgcctattat atggattgct ggtggcactg acaaaggcaa tgaatatgac tgtcttttcg
    13861 agctggtaaa aaacaaggtt aaagctctga tttgtctggg cgccgacaac agcaaactga
    13921 ttgaaacatt cagcccatat gttgaagtaa ttgccgacac acactcaatg gaagaagctg
    13981 taaacgccgc atatcaactg ggaaattcgg gcgatgttgt tctgctttca cctgcctgcg
    14041 ccagtttcga tttgtttgaa aattacgaag accgtggtaa ccaattcaaa aatgccatcc
    14101 gcgaactata gaaatgaagt ttttttcgca acatataaaa ggtgacaaag ttgtatggat
    14161 tatcgtactt ctgttgagtt tattttccat actcagcgtt tacagctcca ccggatcgct
    14221 ggcattccgc taccagggcg gaaacaccga atactatctg cttaaacatg tattcacaat
    14281 tattacaggg cttttcatta tgtatcttat tcacaacatg aaatacaccc tgttttctcg
    14341 tatggcgcag ttgctgttta tattatctgt tcctcttttg ctttatacgc tgataaaagg
    14401 aaccaacgta aatgaagcag cccgatgggt aaccgttctc ggcatcagct ttcaaagttc
    14461 cgatgttgcc aaactggcgg tcattctgtt cctggctcgt gcacttgctc tccggcaaga
    14521 caaaattgac gatctcaaaa gctcctttat ccctatcatt tggccggtag gactggttac
    14581 catactgatt tttccagcca atctttcaac ggcggtgctc gttttcggaa ctgctttgat
    14641 tatcatgtat atcggaagaa ttcctgtcaa atatatagct gctgcaatcg gagccctggt
    14701 tcttgtcggc tctctggcag ttctgattat tatgcaatcg cccggaaaag gtcgaaccgg
    14761 cacatgggca aaccgcatag aatcatactt ttctgatgaa gaaaccgaag gagattatca
    14821 ggcactgcaa tcaaaaattg ccattgccaa cggtggtatt accggaaaac tgcctggaaa
    14881 ttcaacacaa agaaattttc ttcctcaccc ttattccgat tttatttatg cactgattgt
    14941 tgaagaatgg gggctgtttg gaggtatatt cgttttattg ctgtatctga ttttattgta
    15001 tcgtggaatc cgcattgcca cacgaagtcc gggctcgttc ggagcattcc tctctatcgg
    15061 actgaccatc atgattgtat ttcaggcatt aatcaatatg ggggttgccg taaacctgct
    15121 ccccgttacc ggccagccat tgcctttagt cagtatggga ggaacatcca tctggtttac
    15181 atgcatttcg ctgggcatca ttttaagtgt cagcagaaac aaagaagaag aaaaagaaat
    15241 tgccaatgaa aacacaaagg cctaaattta tcttttcagc tggtggaacc ggcgggcaca
    15301 tttatccggc actggcaacg gctgacgcgt tacgcgacct gctgcccgag gccgaaattt
    15361 tgtttatcgg cgccgaaaac aggatggaaa tggaaaaagt tccacatcat ggttatccca
    15421 tcatcggact tcccattcag ggaattcagc gcaaactgac actgaaaaat ctggcagttc
    15481 cattcaaggt tattgccagt ttaataaaag cccgcaaaat tctgaaacag tcaaaacccg
    15541 atgtagtagc aggttttggc ggatatgcaa gtggtccggt attgcgcaaa gcagccagca
    15601 tgggaattcc gacactgctt caggagcaaa attcctttcc tggactcacc aacagaatac
    15661 tatcccgtaa agctgattca atctgcgttg cataccctgg aatggatcag tttttttgcc
    15721 aagaaaaaat taccttgacg ggaaatccta tccgaagtga aattttttct caaagcattt
    15781 ctccagaaga cggccggaaa tttttcggac ttgacacaaa aaaaacaacc atacttgcta
    15841 ttggtggtag tctgggcgca agaaccatca acgaatcgct ctgctcatta atacccgaaa
    15901 ttcttgaaaa agactaccag gtgatctggc aaaccggaaa aaattattcg tataaatcct
    15961 gcaataaatt tgatgcagaa aaaaacaaaa atatctttgt tcaggaattt atttatgaaa
    16021 tgcctcaggc atatgcagct gcagacgtta ttatatcaag agccggcgcc atcgctgttt
    16081 cggaaattgc ggcagttgca aaacccgtta ttttcattcc atcgcccaat gtcaccgacg
    16141 atcatcagac aaaaaacgcc atggtctttg ttaaccacaa agcagcttta atagtaaagg
    16201 ataacgaagc acgtgaatcg ctgagtaaaa tgcttttcac acttatcgat aaccacaacc
    16261 tccgcagtga aatgtgtgaa aacctgaaaa aactggccat gcctgatgca gcaaaaagca
    16321 tcgcaaaaaa aataattgaa ctccgggaaa agaaactgaa tggacattaa aaaaatacat
    16381 attgcatatt ttcttggcat cggcggaatc gggatgagtg ctcttgcacg ttatttcaac
    16441 atgaatggcg ttcaggtttc aggttatgat aaaacatcaa cacccctcac aaaagcgctg
    16501 gaagccgaag gaattgctgt acattatgca gctgacgaaa atgcaatccc ggacaatgcc
    16561 gacattgtag tttacacacc tgcggtgcct gccgactttg cagaatggga aaaaatcaga
    16621 caactcaatt tgcctgttat taaacgctca gccatgcttg ccgaaatcat caacagtatg
    16681 aaatcaatag ctgttgcagg aacccatgga aaaaccacca tttcgggcat gattgcacat
    16741 tcactcgaac acatcggcaa acccttcctt ggatttgtgg gtggtgtttt gtgcggatac
    16801 aacaaaaata ttttcctgca cgaaaatgct gaatgggccg ttgccgaagc cgatgaatac
    16861 gaccgctctt tcctggcgct gcatccgcga attgccatca tcaacgccat cgatgccgat
    16921 catcttgata tctatggaca aatagaggaa ctgcataaat cgttttccga ttttgctgct
    16981 cagacaaatt ccgaaggcca tgttcttctg tatgacagag ccgataaaac aaaactaaaa
    17041 ctaccggaaa ataccatttt atacggtttt gaaaatacgg aaggatactc agcacgaaac
    17101 atccatgttt gcaccaaccg ctttgtattt gacttgtacc taaataacca actgcttgaa
    17161 ggggcaatcc atttaccggc agccggacga cacaatatac aaaatgcaac tgctgcattt
    17221 gctgctatgc atctggctgg ctgcgaaaca acaaaaattg ttcaggccct cgaatcttat
    17281 cccggtgtaa aacggcgact ggagctgatt tcaaagaaca gtaaaattat ttattacgac
    17341 gactatgcac atcatccggc agaaatagat gcactcataa acaccatacg cgaactttat
    17401 cccggcaaaa ccattaccgg aatttttcaa ccacatttgt tttcaagaac ccgcgatttt
    17461 ggaaaggatt tcggacaatc actggccaaa ctcgacgttc ccgttataac cgatatttat
    17521 ccggccagag aaaaaccaat tccaggcatc gatgcaaact ggcttctgga gctgattcca
    17581 aatgtccata aaaaatacat tccgtacaat cagcttccgg aaatcgtgaa cacatgcgat
    17641 gaagggattc tgctcaccat aggtgccggc gatatcgaca tgcatatttc aaccatcaaa
    17701 aaccgcatcg aacaatcatg aagccgatct acaaaatatt gattctgtcg ttagtcatca
    17761 ttgttgtgct ggtgctactc aagttgtcaa atgacaattt caaaaaacaa aaaatcagtc
    17821 aggtcaatat caccatcaac acacagagtg gaccggaact ggtaaccgaa gaagaagtgc
    17881 gaacactcat atcgcagggt tacgattcgt tgaccaaaaa aactgttgaa gaaattgatc
    17941 ttgtatggat tgaaaatata acacgaacaa atccatatgt tctgactgca gatgctttta
    18001 taaatatcga cgcaagtctg actgttgaca ttcttcaacg aaagccgatt ttgcgggttt
    18061 tcaatcaaaa aaatgaatct ttttttatcg atgccgaagg caaaatgctg ccactcaacc
    18121 gtggagcttc accgggtctc attgttgctg caggagacat tcaggagcgt tttcttccat
    18181 cacaaaagta ccagcatttc gactcagtaa gttttgatca gacaaaagtc ttgtcaacga
    18241 tgcacaagtt atacctgatt gcttctgaga tttcgaaaaa tgaatttctt tccagattga
    18301 ttacacagat atacgtcaat gataatacac actacgaact aattcccgtc atcggcaatc
    18361 agaatattat tctcggagac gtggatggtt taaaaatcaa actaaagaat ctgcatcatt
    18421 tctacaaaaa aggatacaac accattaact tttcaaaata taaaagcttc gatatacgct
    18481 tccacaatca ggttgttgga ataaaagaaa cgtacgaaat caacgagata aaaaaagaaa
    18541 acgagatatg aacgaaaatc aggaaataat ggtcggtctc gacatcggaa caacaaaaat
    18601 cgccatgatt gtcggttatc agaatgaaca cggaaaactg gaagttctgg gccatggcaa
    18661 aaccgaatca ttaggtgtaa aacgcggttt ggtagccaac attgacaaaa cagtcgaagc
    18721 cattaaaaaa gtggttgaaa ttactgaagc aaaaagcaat gtaaacatcc ggacagtgaa
    18781 tgtcggtatt gccggtcaac acatcaaaag tctgcagcac cgcggaagca ttatccgtga
    18841 aaacacagac aatgaaattg gccaggaaga tgtggaacgc ctgattgaaa acatgtataa
    18901 acttgttatg cctccgggcg aagaaatcat ccatgtcatt cctcaggaat acattgtgga
    18961 tggagaacaa ggaatcaaag accctgttgg catggctggt gtacagctcg aagctaattt
    19021 tcatatcatc accggccaga ttcttgcggc acgaaatatt gaaaaatgca ttacccgagc
    19081 cgacatttcc atgtcggata tggtgctcga acccattgcc agctcatact ccgtgctcag
    19141 cgaagaagaa aaggaagcag gtgttgcatt ggttgatatt ggtggcggaa ccactgatat
    19201 agccattttt caggatggta tcattcgtca ttctgccgtc attccacttg gcggcgacat
    19261 cgttacagaa gatgtaaaag aaggatgctc cattttaaaa agttatgctg aaacactgaa
    19321 agtgaagcat ggatcagcac ttgcgaatga agtaaaagat gaaatctata tttcaattcc
    19381 cggactgaaa ggccggcctc acaaagaaat ttcaatgaaa aatcttgcac gcattattca
    19441 atcacgcatg gaagaaatca ttgagtttgt aaattacgaa ataaaaaatt cgggctacga
    19501 ccgcaagctc attgcaggaa tagttctgac cggaggcggt gcacaactaa aagacatagc
    19561 acaactatgt cagtttatca caggacacga aacacgcatc ggatatccga gcgaacacct
    19621 gtcggccaat gtggacaaag aattactgtt acccatgtat gccacctgca tcgggcttgt
    19681 actcaacgga atcgaaaact cactcgcttc acaatccaac tcaaaaaaaa caattcccaa
    19741 ccagggaaaa agagaaagag gcggatttat taagaaaatt ttccgcggcg gaatgaactt
    19801 ttttgatgaa aacatcagtt gagtaattaa caattcacaa taatttgctg ttgataagtt
    19861 ttttgagatc aacaggaatt ataagacaaa caaaagtaat tttacccatt aaattaatca
    19921 ctaatatgtc ggaaacggaa aacagtattc atattcaatt tgaagatacc caaagtcttc
    19981 cgaccatcat caaggttttt ggtgttggag gtggtggcag caatgctgtc aatcacatgt
    20041 tcaaacaagg aatcaacgga gttgactttg taatttgcaa taccgatgtg caatcgcttg
    20101 gcaccagtcc agtgcctgta aaacttcaga tcggagccgg tcttaccgaa ggacgcggag
    20161 ccggcaattc acccgaaatc ggccgcaatg cagcgctcga aaacgctgat gaaatccgcg
    20221 agcttctttc gcaaaacaca aaaatggtct tcatcactgc aggcatgggt ggcggaaccg
    20281 gcaccggcgc agctcccgtt attgcgcagg tagccaaaga acttgatata cttactgtgg
    20341 gaattgtaac aactcctttc ggcttcgaag gcggcaaacg taaaaatcaa gccagtcagg
    20401 gcattgatga aatgcgtaaa tatgttgaca gtctcatcat catcaacaac gataaagtac
    20461 gcgaacacta tggcaatctg aaactcagtg aagctttctg caaagccgac gatattctca
    20521 ctgtagcagc caaaggtatt tcagaactca ttactattcc cggttatatg aatgttgact
    20581 ttgaagatgt caaagccatc atgaaaaaca gcggcatcgc catcatggga accggtattg
    20641 ctgaaggcga agaccgcgca cagaaagccg ttgaactggc tcttaattca ccactgctcg
    20701 acgataatga aatttatggt gcacgcaatg ttcttctgtt catctccagt ggtgaagatg
    20761 aaatctccat ggacgaggtc agtgatatct ctgattatat ccagaatgct gcgggctcca
    20821 cagccgaagt tatctggggt actggcaccg atttatcgct tggcaacagc atttctgtta
    20881 ccgttgttgc aaccaacttc aacaaacatt cttcgcctgt taaatccatt tacgaagaac
    20941 ctcaggtaat caatttacaa cctgaagtaa aacaggaaat gccattggct gaaccagaaa
    21001 cggaaatgaa agttgttgtg aaaccggttg aagaaaaacc tgttattaat ctctatcaac
    21061 caccgatcat tcctgccact ccaattgttg atcaacctgt tgaaaaaatc accatacaac
    21121 ttgaacagga acaagaacct gaacagaaca acaatcccat gaatcttttc ccttctgaga
    21181 atgaaacaaa aaccagccct cagccaatga ttcatgatgt tctgcccgcc gaaaaaaatc
    21241 aggaagaacc attgcaaaca cccatccggc ccgaacttca ggatgagcgc atgcaaaatc
    21301 tgaaaaattt caactttaca aatccgaaag caaccaatct cgacgaaatt gaacaggttc
    21361 ccgcttatgc ccgcagaggc gtaaatctgg cccctgagaa tccttcggac gagaaaatag
    21421 tcagcaggtt cactttaggt gaaaacggca gcaataagcc cgaaataaga gaaaataatt
    21481 cattcctcca cgacaatgta gattaatttt cctgacccaa ttaagaacac ttctatattt
    21541 caataaaaat gcgctcttgc ggggcgcatt tttatttact tattacgtca ggtgaaaact
    21601 gaatgcacag aataccatct ctttgattag tcataccgct ataaattatg gaacaacaaa
    21661 cataggcgga ttctacaatt gcattataga tataaaaaac actgtttcgt atagtatata
    21721 ttgaatttga tgatattgat taaatatatt tctattaaaa acataaatat taaaattaaa
    21781 tttatagatt tgccctacta aaccttataa actatgaaaa acttaatgat tgaacccgaa
    21841 tggtggtcgc gtaacgatca ga
//
LOCUS       DAOPKE010000047        21652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2577_length_21652_cov_12.418530, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000047 DAOPKE010000000
VERSION     DAOPKE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2577_length_21652_cov_12.418530"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..2198)
                     /locus_tag="PKY63_07445"
     CDS             complement(465..2198)
                     /locus_tag="PKY63_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00483.1"
                     /translation="MKTKALIIGTVICLLASVNVSAQNDTVNAYTENVVVVGSYDPRL
                     MDFPKKEFSPSFKADDVKKTDMQYSVFAQPMKIAFQTAPIKAAKMSGEPFTELYGNYI
                     RVGFGTGRTPYGELFVNTKRNKTKSFGLHMKHFSTNAKIPDYAFSGMSNNEIDLSGSR
                     IKSKNTLYGKLFFHSDVVHYYGFRPDSVRLPVDSSEWLSTDKKDIRQRYNDAGALISF
                     YSTHADSSHLNYQSDLTYHCFWNIHNATEHSVSLGGYFDKRVSWLRKVSKNQVVGLRY
                     DIEHYFQNRGFTAWNAGLIDLAPYISTNFGPVFIEISPKLVMETDSTAEAHFLPEIRL
                     ELNLLPGILSAFGGLDGNVKYHSFRSLSNENPFIHHFITSDYSKTAQVLYGGVRGNIA
                     HVFNYSIQAVNRKTENCPLFVIDDSFSYLENQMTVVYDEKVSWFRGEFNGALNIGSKW
                     KGLLMVAYNHAEAVTNDEAYNIPDFEGMLGLSYNLADKILASARVYYIGKRYARMTNN
                     DYWVESLPSPLLVTELKPAIDANLTLEYRYSKVLSAYLEFSNIASQRYMIWNHYPSYR
                     FRFLGGVTYSF"
     gene            complement(2203..5268)
                     /locus_tag="PKY63_07450"
     CDS             complement(2203..5268)
                     /locus_tag="PKY63_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00484.1"
                     /translation="MRNTRLLLMVGLLLLHMSSMGQLTREYSDPQAIYLSASDLMMKE
                     KFGAAREAFQKVLDCIPNEEDPMRVEAEFQIARCSYELLNNDARGRLHAFMSRHPESS
                     FVPLAGFYLGSIYYRDKNWKKAAKAYAGISPQHLSSEQQAEYYFKKGYAHFMSEEMLQ
                     ARSCFMQVKDGETHWAAPATYYYGHISYSEGQYEVALGAFSKLVNDETFGGVVPYYIA
                     QIYYLQNKFDELIAFVPPMLENPNVKRQAEMARMAGDAYVAKKDYKQALPYLEQYAAK
                     TSTPLTREDEYLLGYTYYVNTDYQKAAVCFSNVTSMEDSLNQNASYHLADCYLKMGNK
                     KYALNCFNDAYRLKFNTDITEDALFNYARLSYELDFHPYNGAIKALQQYLNDFPKSIR
                     AEEARELLVELLMSTGNYKDAIVVIEGIRVKNERIWTAYQKVNYYHGVELFNSRDYDG
                     AMTLFNKAITYTYDRKIRAEALFWKGETHYRKQQYDSAAVQYDMFVKYPVSSGLSYYA
                     RGYYGLGYAQMQRKNYKNAASSFELYFKNGEKDDQFFRNDACLRLADCYYILREYAQS
                     IEWYEKSLAMNVENMDYALLQKSKCEGVKGNYAAKQNTLSLLFTKYPSSRFSDDAYYE
                     SGITYEIQDQNTQALSSYEKLIADYPESSLRARAMLKKGSIYRILGQNDKAIEAFKQL
                     VSEYKGSEESKQGWMNLKAIYTDMDRIPDWIELVSSQGSSVSQMEKDSMVYLAAENKY
                     LDGDCETSSVAFGKYIEEFPAGAFIQNAHFYRAECLYGQKDFQNAQTGYEFVISNPFS
                     SFTETSLLKSARIYFNAKNYPKAVERYTELDRITQSVSNKQEAQRNIMYAQFNSELYY
                     EAIVSAQKVIDDAAVAPKDKNEASAIIARSSYQLNDLPKAQAEFSKLVKLKSSELGAE
                     ASYYLALIEYQNARYTESEKLVFDLINEFASFEYWVAKSFILLSDVYVAKGDYYQAKY
                     TLQSVIDNYKGEDLVTEARTKLDAIITLEKQKEQQLDGEGEQIIDGNNE"
     gene            5470..5994
                     /locus_tag="PKY63_07455"
     CDS             5470..5994
                     /locus_tag="PKY63_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00485.1"
                     /translation="MKQKFRQGITLQGQWNFAGFIFIVGGFVMMSYRQTFYFGLPLVV
                     LLFPVLLSIRGTDIDYEKKSFRKWTWYYGFKIGKVYSLVGYTFAELRNYYACHTMGCE
                     SISGTYTAKTFEILLGGPNKPKIIFTEIPDYASARKALDEICSQSGLELIDSYEAMLD
                     RVRNLPNTNPDRKR"
     gene            6116..7510
                     /locus_tag="PKY63_07460"
     CDS             6116..7510
                     /locus_tag="PKY63_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPF00486.1"
                     /translation="MRTKYIDLIEQTFEFPQEEFKVVENELLWNDINLMQIIEQYGTP
                     LRISFVPSISRQIQKAKRMFNVAMAKVDYQGEYFYAYCTKSSHFAHVLEEALKNNVHL
                     ETSSAFDIHIVEQLFEENKITKETFIVCNGFKRQHYIENISRLINAGFSNCIPVIDNK
                     EELLEYKIRRGIKANLGIRIAAEEEPMFEFYTSRLGVRYNDILPFYNLYIRNHPNFDL
                     KMLHFFINTGIKDSAYYWSELTKCVNIYVELKRIAPTLDTLNIGGGFPIKNSLAFDYD
                     YEYMAEEIIAQIKNICNQNKVPEPNIFTEFGSYTVGESGAVLYSILGQKQQNDREMWN
                     MIDSSFMTTLPDTWALNQRFILLAVNHWDYDYQRIFLGGLTCDSHDYYNAEAHANAIF
                     LPKIEPNDPQYIGFFHTGAYQESLGGFGGIQHCLIPAPKHILILEDPETGEYYPKLFA
                     KEQSYKSMLKILGY"
     gene            7913..8593
                     /locus_tag="PKY63_07465"
     CDS             7913..8593
                     /locus_tag="PKY63_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00487.1"
                     /translation="MKKLALIIVVALITGFAGNADAQKPFKGILTYTITYDGTWDAAT
                     LAQQPTETKMKILGKYSKSEMIAPGATVENITNGKDSSQIVLLDIPMLGEKYYMLTKK
                     DKILESLAENKAPEINYLDETKVIAGYTCNKAEYITEDEYGDKKTTVVYYSTAIGGPE
                     LNFGGQFTGLKGFPMEYVMETDEGKITFTVSSVQVKKVKLKETDFYIPTDYSELTEEK
                     AKELFGGE"
     gene            8688..11276
                     /locus_tag="PKY63_07470"
     CDS             8688..11276
                     /locus_tag="PKY63_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPF00488.1"
                     /translation="MKKNRQDKPLSNSRKESKNSARNTIKAIHKNKNTSKKEQSKSNK
                     TKSESKKKEFGNDRKSFMEFLKSKETHFGFGLFLLVFSVFLMFSFISYLKNWHSDDNV
                     VQSGLRILFDSAVTVENSMGKLGAWFGCLFIKKWFGIGSFLLLIFFINSGLLLLRVKL
                     VGFFESVLYLIGTTLWLSVFMSAIFTRPDAMIWGGLFGWQIRDWISGAIGNFGLIILL
                     LVVFILYISTLFGIKLHEIKLRKKTTENENNDNNISISGQNNETEIANTKTQIDDLSV
                     IEKNPDEMIQEPQHQDVPEFELTEKVTVFNDSDEKVETGGIDLTVPELIKNQNEKKDD
                     DVQFVIETSPVELAPVLADEPEHFGLDTLYDPLKDLSQYQMPPTTMLIDRGEGESKVG
                     REELENNKNRIVETLNNYDIKISQIKATIGPTVTMYEIVPAPGVRISRIKNLEDDIAL
                     SLAALGIRIIAPIPGKGTIGIEVPNLTRETVSLRSLLESDKFTGTKAELPFAMGKTIT
                     NEPFVADLAKLPHLLIAGATGQGKSVGLNAIICSLLYKKHPSQLKFVFVDPKKVELAL
                     YAKIEKHFLAKLPDADDAIITDVTKVIATLTSLTQLMDSRYDLLKNAGCKNIIEYNHK
                     FLERRLNPNDGHMYMPYIVLVIDEFADLIMTAGKEVELPVARIAQLARAVGIHLIVAT
                     QRPSVDVITGKIKANFPARIAFKVSSKVDSRTILDMNGADQLIGMGDMLYSHGSTMLR
                     LQCAFVDTPEIEALTDFIGEQQGYPITYELPEPIVEKDTVKGDADLKNKDKLFNDAAH
                     IVVTLQQGSTSLLQTKLTIGFARARRIIEQLEQAGIVGPPQGSKPRDVLVKDIEVLEQ
                     FLKNSE"
     gene            11301..11939
                     /locus_tag="PKY63_07475"
     CDS             11301..11939
                     /locus_tag="PKY63_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPF00489.1"
                     /translation="MKKIFLIAFVFASTIFNASAQTDTKAKAILDALSAKLDALETMK
                     FEFSYTMTNTAENINETKTGSIYIKDDKYRLYIADQLIICDGTTIWTILKEDKEVQIN
                     SVDPTNQNTPNKMLTSYNKNYKAKFIKEAPKAGIIMQTLDLTPLTAQSFYKVRLEINK
                     SKNMVYSSTIYDKNGTTYTYLVTNFIENPKVFESRFTFNKADYPDYSINDMR"
     gene            12020..13918
                     /locus_tag="PKY63_07480"
     CDS             12020..13918
                     /locus_tag="PKY63_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPF00490.1"
                     /translation="MRKYLLFAILIFAAATSFGQLLTPTVDSIPMRDGKKLAADIYLP
                     DTTGGISYPVILIQTPYNRLWYHFGLPLIGTAIDTSPFAYVILDWRCFYGSAAACVTT
                     PDRGADGYDAVEWIAAQSWSDGQVATWGPSALGRVQYMTAKKNPPHLVCAVPLVAGPQ
                     YNYLEYFPGGVARDEYIDQLDALGYGMSGSLYANPHYNLLWSYSESTNFYPDSIQIPL
                     LMIGGWYDHNTVVMLDFFDAIRTSSPAAVRDKHKLLMGPWVHGGHGAAYVGTDSAGQL
                     SYPMAAGISDSLAMRFLKFYLLGEANGYDTLSTITNFQMGVNTWQYIENWPQSGITET
                     NLYLHEDLSLQPVSPSGWFGNRGFSYDPRDPSPTVGGPTLRADMVQGPYDQSPTVESR
                     GDILVFTTPELESDATVQGYPKVVLYVKSDCRDTDFAARLTDVYPDGRSMLLVDGIRR
                     ARFREGYTTSDTIFMNDSSTVYQVEIELNPTSHTFLAGHRIRLDITSSNYPRFNNNMN
                     DGGPMYVAGDTTVAHNRVLCNGTSPSKLVLPVDVIDVGVEKNRKPELSIFPNPCSNEL
                     FVTIPDGIINNIVVVSVYDATGKKISAKSDIEGNIVKINTSSLAAGLYTIQLQSDVQC
                     FTVTFFKK"
     gene            13978..15069
                     /locus_tag="PKY63_07485"
     CDS             13978..15069
                     /locus_tag="PKY63_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00491.1"
                     /translation="MRSTIFIFLLLLAGIVDAQTGEYYFIVHPFEFDEIYMTAKAQQT
                     RLASKVKTITETTTTYKKGNPEKATLKTVFTLNQKGETIQKEEYDAKGQLMRKHSIML
                     NDSGWVEQKKIEDGKGKVLFQYDKKYDPLTGNIISYEYRKNDKLKLKYTKSYCGKNLA
                     EQIVYKKDGETVSRRFVYTYNTESKLQITHLYDGNGKLLHTWNHDCLPEGELMAARKD
                     TTTVCKMREESADGHFVNYTKSVNEKGRLTTTTTWFRAEYKADSVKVVTEGKHPSTRF
                     YRYGADGKQFESIVMNDENIITHKYFQLMSENGNQLKYENWSYSKKHGLRCTNKAISE
                     ENAQGLLTKKTYYWYGKLKSETSFSYTYF"
     gene            complement(15159..16541)
                     /gene="fumC"
                     /locus_tag="PKY63_07490"
     CDS             complement(15159..16541)
                     /gene="fumC"
                     /locus_tag="PKY63_07490"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPF00492.1"
                     /translation="MEYRKEKDSMGLVDVPADKYWGAQTQRSKDNFRIGGTLMPTEII
                     EAFAILKKAAALVNNDLGKLSADKAKLIAEVCNEISMGKLNDQFPLVVWQTGSGTQSN
                     MNVNEVIANRAHVIAGGKLTDETKTLHPNDDVNKSQSSNDTFPTAMHIAAYSMLVWHT
                     IPALEKLRDALQAKSEAWKAIVKTGRTHLMDATPLTLGQEFSGYVSQLDHGIRAIENS
                     LMHLSELPLGGTAVGTGLNATAGYDVKVAAKIAELTKLPFITAENKFESLSAHDAIVE
                     ASGALKTVAVSMMHIANNIRLLASGPRCGIGEILLPENEPGSSIMPGKVNPTQPEAVT
                     MVCAQVIGCDAAITAGGMGGHFQLNVFMPVMIYNFLFAARLIGDACHSFTDNCVAGIE
                     PNLAAIRKNLESSLMLVTALNTHIGYDNAAIIAKKAHKEGKTLREAAIESGLVTPEQF
                     DEWVDPGKMV"
     gene            complement(16890..17234)
                     /locus_tag="PKY63_07495"
     CDS             complement(16890..17234)
                     /locus_tag="PKY63_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPF00493.1"
                     /translation="MKISVYHNPRCRKSRAGLDLVKSKTDEIDIIDYLNTGISAACIK
                     KLVQQLNVPIIDIVRTQEDYYKKELKGKNFSGEEWAQIISENPKLLKRPIVVKGNKAA
                     IVESVDDLERLF"
     gene            complement(17280..17510)
                     /locus_tag="PKY63_07500"
     CDS             complement(17280..17510)
                     /locus_tag="PKY63_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00494.1"
                     /translation="MQKAYFTLTPYLVLSVDAGKTTWNIYAGVVFSNEILRHTGSTDH
                     VNPKTFDYIIDRHPAMASVVLGMEIGLKWKKK"
     gene            complement(17561..18433)
                     /locus_tag="PKY63_07505"
     CDS             complement(17561..18433)
                     /locus_tag="PKY63_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00495.1"
                     /translation="MKYVCIVILACLFASCNPYIVQKAYFVEPPVTATFDSIHQTKVN
                     LTGYYFNHTDLQIMHSFSEHHGVYATGLIGYQGWLSNDEYYGFHDKGVPNLTGGFSGG
                     YIWYSQRPKSYMECLAGYGFQINNARFYEKWISPMSLLGGPYFSQDVHSAFHRFSVQP
                     SIFSNKKNSWGFSLRAELVYCPYYYYDYEVAFYDPEHDDYPVSSDYSDRITFYNKWFS
                     VLTPVFTYQNRSGKTPYGFYIGANFPSLVKKEYNDLSHYQDYTWGYVLKKHPTSANII
                     FGFQYTLPLNLRKQ"
     gene            complement(18512..19549)
                     /locus_tag="PKY63_07510"
     CDS             complement(18512..19549)
                     /locus_tag="PKY63_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00496.1"
                     /translation="MKKILFGLCLLFFAAAVQAQVAGSTQKLDVSKWKQFRYLGEEYW
                     KDSLYIFSRNAWITIKCYDLGFLADPNMQMSLLLDSVQQHYSTKSNYYDKTQRFKLRY
                     YNPGFLVMEQLDSTKKTVYSEKRQSIIPYPELNECRIDIPGGFINIRFYNDSVLLSPM
                     LKALITDVIALKSQKDWHRASEVRATNIDYSDSTFRADKKPYIIYSRKKMDEKIVLNL
                     SSGLCLSKAPYSLSAEVKLGYIWNNFDYDIGYYLSYDWNFSFAENGTRSINSFINLSA
                     LEEGFLPRLKTMAGIDMGYLVFRQGDVFEKNTFRLGFGFKGDFWYFSPQMYFPGKFKG
                     VYPGLRLLIGI"
     gene            complement(19587..20213)
                     /locus_tag="PKY63_07515"
     CDS             complement(19587..20213)
                     /locus_tag="PKY63_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00497.1"
                     /translation="MKTSEFDNIIRQALLEEAANDDAAAEAARGRVWQKANPPKMFSL
                     TAVLFRVAAAAILVLLLTTAYFMWIQKEHSAKKMAEKKEIRNAANSLDSLVKPAKNVV
                     FQTIYLDRFLTSAIVHDTIILQKTDVVYSTSHDTIFIEKQHTSEPLIAGGNPQVNDVF
                     PIEYTPQKQVRGILFWRTKPAFPNNTATADVRYDRKKQTFTIPVISAQ"
     gene            complement(20200..20745)
                     /locus_tag="PKY63_07520"
     CDS             complement(20200..20745)
                     /locus_tag="PKY63_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF00498.1"
                     /translation="MNEDKLIRQCKSGSRSAQRELFDAHYRTSFRIAVRYLGNHHDAE
                     DVVADAFIRVFNGLHTFDVRTEGSLGRWIHTLVINEALRFLKRRRLLVLEEDMALYDD
                     YASESFDASVFDAEQIKQVLTTMPAGYRTVFNLYAIEGFSHKEIAQLIGISETTSRSQ
                     LCKARSFIIQRITNNVSHENV"
     gene            complement(20822..21541)
                     /locus_tag="PKY63_07525"
     CDS             complement(20822..21541)
                     /locus_tag="PKY63_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPF00499.1"
                     /translation="MEIFLTPEAWISLLTLTLLEIVLGVDNILFISIVSNSLPADQQR
                     PAQRIGLVVAMFLRVALLIALVYLMRQMTEPLFYVNEMGFSFREIILAAGGLFLVAKS
                     VSEIHGRMETGPVQTKKDIGKKFFNVVLQIVLLNIVFSFDSILTAIGLSNQLLIMISA
                     IVISMFVMIAFSGSISRFIAKNPTLEILALSFLILIGFMLMLEGVHIEVPKGYIYFAV
                     FFSLIVELVNMKIRKKSLAKK"
BASE COUNT         6479 a         4881 c         4241 g         6051 t
ORIGIN      
        1 ttgatgaata tatttctgtt tttgtgagtg ttacgtgttt ttcagcagac cctatgtaaa
       61 cattaaaaga cttattgatt gcaaacaaaa tgaggttttt cgaggtgccc ttaatgaatt
      121 tgtcccgatt gatattttat ttttcattta gtctcatgtt gctttgcaga gcttctaacc
      181 atggaatgct ggattgcttt gcatatctac aaacagagat gttcatacgt cccgtcaggg
      241 acggcatatt ggtagaaaaa aacagcatct ttgttcgtcc tgtaaggacg aaacagtctc
      301 attaaataaa ccattccgta cctggtaacg aagcttttta gatccgactt cgccagaatt
      361 ctccaaaata gcttcaacca acagatcata tacgatggta ttgcacccga caattttacg
      421 ccaggagttt atcccgacta aggcggggcc ttacacctaa taatttaaaa cgaatacgtc
      481 acaccgccca gaaaacggaa gcgataggat ggataatgat tccaaatcat atatcgctgc
      541 gaagcaatgt tgctgaattc aaggtaagca gacaatactt ttgaatagcg gtattccaat
      601 gtgagattgg catcaatggc tggcttgagc tctgtaacaa gcaaaggcga cggcaaagac
      661 tcaacccaat aatcattatt ggtcattcgg gcatatcttt ttccaatgta ataaacccgt
      721 gcgctggcca gaattttatc ggcaagatta taactcaatc caagcatccc ttcaaaatcg
      781 ggaatattat aagcttcgtc gttggtaaca gcttctgcat gattgtaagc caccatgagt
      841 aaacctttcc atttgcttcc gatattcaat gccccattaa attcaccacg gaaccaactc
      901 actttttcat cataaaccac agtcatctga ttttcgagat acgaaaacga atcatcaatt
      961 acaaagagag ggcaattctc agttttacga ttaacggcct gaatactgta gttgaaaaca
     1021 tgagcgatgt ttccgcgaac gccaccatag agtacctgag cagtttttga ataatcactt
     1081 gtaataaaat gatgaataaa aggattttcg ttagacagag aacggaatga atgatattta
     1141 acgttgccat caagcccccc aaaagcgctc agaataccag gaagcaaatt taattcaagt
     1201 cttatttcag gcaaaaaatg agcttctgcc gttgaatctg tctccatcac caactttggt
     1261 gatatttcaa taaacacagg accgaaattg gtgctgatat aaggcgccag atcaatcaaa
     1321 cccgcattcc atgcggtaaa tcctcgattc tgaaaataat gttcaatatc gtatctcaac
     1381 ccaacaacct gatttttact gacttttcgt aaccatgata cacgtttgtc aaaataacca
     1441 ccaagcgaaa cactgtgttc cgttgcatta tgaatattcc agaaacaatg ataagtaaga
     1501 tctgattgat agttcagatg cgacgaatcg gcatgggtgc tgtaaaatga aatgagtgca
     1561 cctgcatcgt tgtaacgttg cctgatgtct tttttatcag tcgaaagcca ctcggatgaa
     1621 tcaaccggaa gtcgcaccga atcaggcctg aagccataat aatgaaccac atcgctatga
     1681 aaaaacaact tgccgtataa tgtattcttt gattttatgc gcgatccgct caaatcaatt
     1741 tcattgttgc tcattccgga aaaagcataa tccggaattt ttgcattggt tgaaaaatgc
     1801 ttcatgtgca atccaaaact ctttgtttta ttgcgcttag tgttcacaaa cagctctcca
     1861 taaggcgtac ggcctgttcc aaatccgact cggatataat ttccgtacaa ctctgtaaaa
     1921 ggttccccac tcatttttgc tgctttgata ggcgcagtct gaaatgcaat cttcatgggc
     1981 tgcgcaaaaa ccgaatactg catatcagtc ttcttgacgt catcggcttt aaacgatgga
     2041 ctgaactctt ttttcggaaa gtccatcaat cgtgggtcat acgagccaac cacaactaca
     2101 ttttctgtat aagcgttcac agtatcgttt tgagccgaaa catttacgct tgctaaaaga
     2161 caaatgactg tcccaataat cagtgctttt gttttcattg tactattcat tatttccgtc
     2221 aatgatttgt tcaccttctc cgtcgagctg ctgctccttt tgtttttcca gcgtgatgat
     2281 agcatccagt tttgtccggg cctctgtaac aagatcttcg cccttgtaat tgtcaatcac
     2341 gctctgcaac gtatatttgg cctgatagta atcgcctttt gccacataaa catcactcag
     2401 cagaatgaac gatttggcaa cccagtattc aaacgatgca aattcgttga tcagatcaaa
     2461 caccagtttt tccgattcag tataacgtgc attctgatat tcaatcaaag ccagataata
     2521 actggcctct gcgccaagtt ccgatgattt aagttttacc agtttactga attcggcctg
     2581 tgccttcggt aaatcattca gctgatatga agagcgtgca atgatggccg atgcttcgtt
     2641 cttatctttt ggcgcaaccg cagcatcatc aatcactttc tgcgccgaca cgatggcctc
     2701 gtagtacaat tcgctgttga actgagcata catgatgttg cgctgagctt cctgcttatt
     2761 cgatactgac tgcgttatcc gatccagttc ggtatatctc tccactgctt tcggataatt
     2821 ttttgcattg aaataaatcc gcgccgattt caataatgaa gtttcagtga acgaagaaaa
     2881 aggattggat atcacaaact cataccccgt ctgtgcattc tgaaaatcct tctgcccata
     2941 caagcattca gcacggtaga aatgcgcatt ctgaataaat gctcctgctg gaaattcttc
     3001 tatatatttt ccaaatgcca ccgacgatgt ttcgcagtca ccatcgagat atttgttttc
     3061 agcggcaaga tataccatgc tgtccttttc catctgcgat acagaactgc cttgcgaact
     3121 cacaagttca atccagtcag gaatgcggtc catatcggta taaatggctt tcagattcat
     3181 ccacccttgt ttcgattctt ccgaaccttt atattcactc accaattgct tgaatgcttc
     3241 aatggctttg tcgttctgac ccagaatacg gtatatactg cctttcttca acatggcgcg
     3301 tgcacgcaat gaactctcag gataatcggc aatcagcttt tcatatgaag aaagcgcctg
     3361 tgtgttctga tcctgaattt cataggtaat acctgattca taataggcat cgtccgaaaa
     3421 ccgcgatgaa ggatattttg taaaaagaag cgacaacgtg ttctgctttg ctgcataatt
     3481 gcctttcact ccttcgcatt ttgatttctg aagcaatgca taatccatat tttcaacatt
     3541 catggccagc gatttctcat accattctat cgattgtgca tactcacgca aaatataata
     3601 gcaatcggcc agacgaagac aggcatcgtt tctgaaaaat tgatcatcct tttctccatt
     3661 tttaaaatac aactcgaaac tcgaagccgc atttttgtag tttttacgct gcatctgcgc
     3721 atatccaaga ccgtaataac ctctcgcata ataactcaat cccgatgaaa ccggatactt
     3781 cacaaacatg tcatactgta ccgcagccga atcatactgc tgctttcggt aatgtgtttc
     3841 acctttccag aacaaagctt ctgctctgat tttccggtca taggtgtacg tgattgcttt
     3901 attaaaaagt gtcattgcac catcataatc tctggaattg aacaactcaa cgccatgata
     3961 ataattcact ttctgataag ctgtccatat acgctcattc ttcaccctga tgccttcaat
     4021 tacaacaatg gcatctttat aattaccagt tgacatcagc agttccacca gcaattcgcg
     4081 cgcttcttca gctcgtatcg atttcggaaa atcattcaaa tattgctgca acgccttaat
     4141 agcgccatta taaggatgaa agtcaagttc atatgacaaa cgggcataat tgaacaacgc
     4201 atcttcagta atgtcagtat taaatttcaa acgataggca tcgttgaaac aattgagcgc
     4261 atatttttta tttcccatct tcagataaca gtcggccaaa tgatagctcg cattctgatt
     4321 gagcgaatcc tccattgaag taacatttga aaaacacaca gccgctttct gataatccgt
     4381 attcacatag tatgtgtatc ccagcaaata ctcatcttca cgtgttagcg gcgtagaagt
     4441 tttggcagca tactgttcca gatatggcag tgcctgttta taatctttct ttgccacata
     4501 ggcatcgcct gccatccttg ccatctcggc ctgtctcttc acattgggat tttcgagcat
     4561 gggaggaaca aaagctatca attcatcaaa cttattttgc aggtaataaa tctgagcaat
     4621 ataataaggc acaacgcctc cgaatgtttc gtcattaacc agcttgctga atgctcccag
     4681 cgccacttcg tactgtcctt cggaatagct gatatgtccg taataatatg tggctggagc
     4741 cgcccaatgg gtttctccat ctttcacctg cataaaacaa ctgcgcgcct gcaacatctc
     4801 ttcgctcata aaatgcgcat atcctttttt aaaataatac tcggcctgct gctccgacga
     4861 taaatgctgt ggactaatgc ctgcataagc tttggcggct ttcttccagt ttttatcgcg
     4921 ataataaatg ctgcccaggt aaaaaccggc caagggtaca aaactactct ccggatgcct
     4981 cgacataaat gcatgaagcc ggccacgggc atcgttgttc aatagctcat acgaacagcg
     5041 cgcaatctga aattcagctt caactcgcat cggatcctct tcattcggaa tacaatccag
     5101 cactttctga aatgcttccc gcgctgctcc gaatttttcc ttcatcatca ggtcactggc
     5161 agaaagataa atggcctgtg gatcactgta ttcacgtgta agctgaccca tggatgacat
     5221 gtgcaacaac aacagtccaa ccatgagtaa taatcgtgtg tttctcatat ctgtgaaaga
     5281 ttttgaacga taaaattact tcgataacaa attgcttatt tattagaggt gcaaacttat
     5341 caacaacagt tttttaatat tctgctgata ttgtttgcaa atcacaggta taagcgctgc
     5401 ttcattccga ttcaatataa ttactatttt tacaaaacgt ttgcagcaat caaatatttc
     5461 acctcagcat tgaaacagaa attcagacag ggaatcacac ttcagggaca atggaacttc
     5521 gccggtttca tttttattgt tggtggattt gtcatgatgt catacaggca aacattttac
     5581 ttcggactgc ctctggtggt tttactcttt ccggttttac tgtcaatccg tggcaccgat
     5641 atcgattatg aaaaaaaatc attccggaaa tggacgtggt attacggttt taaaattggt
     5701 aaagtatatt ctcttgttgg atatacgttt gccgagctgc gaaattatta cgcctgtcat
     5761 accatgggct gcgaaagcat ttcaggcaca tacacagcca aaacgttcga aattcttctt
     5821 ggtggcccaa acaaaccaaa aatcattttc actgaaatac ccgactatgc ttcagcgcgc
     5881 aaggcacttg atgaaatctg ttcacagtca ggactggagc tcatcgattc atacgaagcc
     5941 atgctcgatc gggtaagaaa tttaccaaat accaatcctg accggaagcg ataagccact
     6001 gtctcaattg tcttttttaa agacactcaa cttttttatc ccacaaaaaa gtaaatgtct
     6061 tacatttgca aaaaatagca aaagtaactc acccggattt cgaaaagcgc cttaaatgag
     6121 aacaaaatac attgacctga ttgaacaaac atttgaattt ccacaggaag aattcaaagt
     6181 ggttgaaaat gaactgttat ggaacgacat caacctgatg cagattattg aacaatatgg
     6241 aacaccgctg cgcatttcat ttgtacccag catcagccgc cagattcaaa aagccaagcg
     6301 tatgttcaat gtcgccatgg ccaaggttga ctatcagggt gaatacttct atgcatactg
     6361 caccaaaagc agccattttg cccatgtact cgaagaagca ctgaaaaaca atgtccatct
     6421 cgagacatca agtgcattcg atattcatat tgttgaacaa ctttttgaag agaataaaat
     6481 caccaaagaa acttttattg tctgcaacgg attcaaacgt cagcactaca tcgaaaacat
     6541 ttctcggctc atcaatgccg gattttcaaa ctgtattccg gtcatcgaca acaaggaaga
     6601 actgctcgaa tacaagatcc ggcgcggcat aaaagcaaat ctgggtatcc gcattgctgc
     6661 cgaagaagaa cccatgtttg agttctatac atctcgcctg ggcgttcgct acaacgatat
     6721 tttgcctttc tacaatctct atattcgcaa tcatccgaat ttcgatctta aaatgctgca
     6781 tttttttatc aataccggca ttaaggattc ggcctactac tggagtgaat taacaaaatg
     6841 cgtaaacatc tatgtcgagc tcaaacgcat tgctccaaca ctcgacacac tgaacatcgg
     6901 tggcggcttc cccatcaaaa attcattggc attcgattat gattatgaat acatggctga
     6961 agaaatcata gcccaaatta aaaatatctg taatcagaac aaagtgccgg aacccaatat
     7021 ttttaccgaa tttggttcat acacggttgg agaaagcggc gctgtgctct attccattct
     7081 tggacaaaag caacaaaacg accgtgaaat gtggaacatg attgacagca gttttatgac
     7141 gacactaccc gacacatggg ctctcaatca acgttttatt ctgctggctg tcaatcactg
     7201 ggactatgat tatcaacgca ttttcctggg cggactaacc tgcgacagcc acgattatta
     7261 caacgccgaa gcgcatgcca acgccatttt tcttccgaaa attgaaccca acgacccgca
     7321 atacatcgga ttctttcaca ccggcgccta tcaggaatca ctgggtgggt tcggcggaat
     7381 tcagcattgc ctcattcccg ctccaaaaca cattcttatt ctcgaagacc ccgaaactgg
     7441 cgaatattat ccaaaacttt tcgccaagga acaaagctac aaatcaatgc ttaaaattct
     7501 gggctactga taaactgcat cgcgacaaat taaggttaag gttaaggtta aggttaaggt
     7561 taaggttaag gttgaggttg aggttgaggt taaggttgag cagctttcag tcatcagtaa
     7621 tcagcattca gcagtaggtg ccgaccgaag cgtcggtgct ctcagaagaa gcttagcagt
     7681 tttaaattat ttccagaatc taatattctc acattttcac ataagcgaag cggaatttaa
     7741 tcctccgcct ccagcggagt aaactccaaa ggcggatcat ttccacatta gcatagccgc
     7801 atgcccaatg ccgcaggcac tattctacca accgccctct aataccgaca aacaaaatga
     7861 aaaaattcag attggtacaa ttttttatgt atttttgcct caaactcaaa atatgaaaaa
     7921 gttagcactg attattgtcg ttgcattgat aaccggtttt gccggaaatg cagatgcaca
     7981 gaaacctttt aaaggaattc tcacctacac cattacctat gatggaacct gggatgccgc
     8041 aacacttgcc cagcaaccaa ccgaaacaaa aatgaaaatt ctcggcaaat acagcaagtc
     8101 tgaaatgatt gcaccgggtg ctaccgttga aaacatcacc aacggaaaag actcttcaca
     8161 aattgtctta ctcgacattc ctatgcttgg agaaaaatat tacatgctca caaaaaaaga
     8221 caaaattctt gagagtcttg ccgaaaacaa agctccggaa atcaactatc tcgacgaaac
     8281 aaaagtgatt gccggctaca cctgcaacaa agccgaatac attaccgaag atgaatacgg
     8341 tgacaaaaaa acaacagtgg tttattattc aacagccatt ggtggtccgg aactcaactt
     8401 tggcggacaa tttacaggcc tcaaaggttt tccgatggaa tatgtaatgg aaaccgatga
     8461 aggtaaaatc acttttaccg tttcgagcgt tcaggtgaaa aaagtaaaac tcaaagaaac
     8521 tgatttctac atcccgactg attattcaga acttaccgaa gaaaaagcaa aagaactttt
     8581 cggaggcgaa taaaaagatt tacacaataa attgttcaaa aaaaccttct cttttcaggg
     8641 aaggtttttg tttttcaatg cgttgcttta agtatttttg cattatcatg aaaaagaaca
     8701 gacaggataa acccctttca aattcacgca aagagtcgaa aaattcggcc cgtaacacca
     8761 ttaaagccat tcataaaaat aaaaacacct caaaaaaaga acaatcaaaa tcaaataaaa
     8821 ccaaatcaga atcaaagaaa aaagaattcg gtaacgatcg taaatcattc atggaatttc
     8881 tgaaatcaaa agaaactcat ttcggatttg gtttgttttt acttgttttt agcgtgtttc
     8941 tgatgttctc ttttatctca tacctcaaaa actggcacag cgacgacaat gtagttcaat
     9001 ctggtcttcg aattttattt gactctgctg ttactgtcga aaacagcatg ggcaaactgg
     9061 gcgcctggtt cggttgcctt tttataaaaa aatggttcgg tatcggctct tttctgctgc
     9121 tcatattttt tatcaacagt ggtttgctgc tgcttcgtgt gaaacttgtc ggcttttttg
     9181 aaagtgtctt gtatctcatc ggaaccactc tgtggctgtc cgtttttatg tctgcaatct
     9241 ttacccgacc cgatgccatg atctggggag gccttttcgg ctggcaaata cgtgactgga
     9301 tcagcggtgc aataggaaat ttcggactca ttattctgtt acttgttgtt tttattcttt
     9361 atatttctac tctttttgga ataaaactgc acgaaatcaa actgcgcaaa aaaacaactg
     9421 aaaacgaaaa caacgataat aatatctcaa tttcgggtca aaacaacgaa actgaaattg
     9481 ccaatacaaa aactcagatt gatgatcttt ctgtcattga aaaaaatccg gatgaaatga
     9541 ttcaggaacc tcaacatcag gatgtcccgg aatttgaatt aacagaaaag gttaccgttt
     9601 tcaacgacag tgatgaaaaa gtcgagaccg gcggaatcga cctcaccgtg cccgagctga
     9661 taaaaaatca aaacgagaaa aaagacgatg atgtgcaatt cgtcatcgaa accagtcctg
     9721 tcgaactggc tcctgtgctc gctgatgaac cagagcattt cggtctcgac acattgtacg
     9781 atccattgaa agatctgagt cagtatcaaa tgcctcccac aaccatgctc atcgaccgtg
     9841 gcgaaggcga aagcaaagta ggacgcgaag aacttgaaaa caataaaaac cggattgttg
     9901 aaacactcaa caattacgat atcaaaatca gtcagatcaa agcaaccatc ggacctacgg
     9961 taaccatgta cgaaattgtg cctgcaccgg gtgtccgcat cagccgcata aaaaatctgg
    10021 aagatgatat tgccttaagt ctggccgcac ttggaatccg catcattgca cctattccgg
    10081 gcaaaggaac cattggcatt gaagtgccta atctgacccg tgaaacagtg tcactgcgct
    10141 ctttgcttga aagcgacaaa tttaccggaa ccaaagctga gcttcctttt gcaatgggta
    10201 agaccatcac caatgagcca tttgtggccg atcttgcgaa gcttccacac ttgctcatcg
    10261 ccggtgcaac cggacaagga aaatcggtcg gactcaacgc cattatctgc agtctgctct
    10321 acaaaaagca tccaagccag ttgaagtttg tatttgtcga tccgaaaaag gtggagctgg
    10381 ctctgtatgc gaaaatcgaa aaacattttc tggccaagct gcccgatgcc gacgacgcca
    10441 tcattaccga tgtaacaaag gttatcgcca cacttacatc tctcacacag ctcatggaca
    10501 gccgctacga tctgcttaaa aatgccggct gcaaaaacat cattgaatac aatcacaaat
    10561 ttctggaacg cagactcaat ccaaacgacg ggcatatgta catgccctac atcgttttgg
    10621 tcattgatga atttgccgac ctgattatga cagccggaaa agaagtggaa ttgccggttg
    10681 cacgaattgc tcaactcgcc cgtgctgtgg gcattcatct gattgtggca acacaacgtc
    10741 cgtcagtcga tgtgattaca ggaaaaatta aagcgaactt ccctgcacgc attgctttca
    10801 aagtttcttc gaaagttgac agtcgcacca tccttgatat gaacggagcc gatcaactga
    10861 ttgggatggg cgatatgctg tattcgcatg gcagcaccat gcttcgtttg caatgtgcct
    10921 ttgttgatac acctgaaatt gaagccctga ccgactttat cggcgaacag cagggatatc
    10981 cgattacata tgaattaccc gagcccattg tggaaaaaga cactgtaaaa ggagatgctg
    11041 atcttaaaaa caaggataaa ttatttaatg atgcagcaca tattgtagtg acactgcagc
    11101 aaggatcaac ttcactttta cagacaaaac taacgattgg cttcgccaga gcacgaagaa
    11161 tcatcgagca actggaacag gcaggcattg taggaccacc ccagggaagc aagccccgcg
    11221 atgttctggt taaagatatt gaagttttgg aacagttttt gaaaaattcg gagtaacttt
    11281 gaaccccaaa tttctcattt atgaaaaaaa tctttttgat tgcgtttgtt tttgcgtcaa
    11341 ccatttttaa tgcaagcgca caaaccgaca ccaaggcaaa agccatcctc gacgctctca
    11401 gtgccaaact cgatgccctg gaaaccatga agttcgaatt ctcttacacc atgaccaata
    11461 cggcagagaa tatcaatgaa acaaaaacag gcagcattta catcaaagac gacaaatacc
    11521 gcctctatat tgctgaccag cttatcattt gtgacggaac aaccatctgg actattctga
    11581 aagaagataa ggaagttcag ataaacagcg tagatccaac caatcagaat acgcctaaca
    11641 aaatgctgac ctcctacaac aaaaattaca aggcgaaatt tatcaaagaa gctccaaaag
    11701 ccggcattat tatgcaaacg cttgacttaa ctcctcttac agcccaatct ttttacaaag
    11761 ttcgtctcga aataaataaa tcaaaaaaca tggtgtattc ttccaccatt tacgataaaa
    11821 acggaacaac atatacctac ctcgttacca actttatcga aaaccccaaa gtatttgaat
    11881 cccgcttcac atttaataaa gctgactatc ctgattattc aatcaacgat atgcgataag
    11941 cattcaaaat attctgaaag ctcccttgag gagctttttt tatgcttcat gaaatatttt
    12001 ttacctttaa aaaaaaatca tgaggaaata cctgctcttt gcaatactca tctttgctgc
    12061 tgcaacttcc ttcggtcaat tgctgactcc aactgtggac agcatcccca tgcgtgacgg
    12121 taaaaaactg gctgccgata tttatttgcc agacaccacc ggaggcattt cttatccggt
    12181 cattctgatt cagactccct acaaccggct ctggtaccac tttggactac cactgattgg
    12241 cacagcgatc gacacaagtc cgtttgctta tgtgattctc gactggcgtt gcttttacgg
    12301 atccgcagcg gcctgcgtga ctacacctga tcgcggtgcc gacggttacg atgccgttga
    12361 atggattgca gcgcagagct ggagcgatgg tcaggtggct acctggggac cttcagcctt
    12421 gggacgtgtg caatatatga ccgcgaaaaa aaatccgcca catctggttt gcgcagtgcc
    12481 acttgtggcc ggccctcaat acaactatct ggaatatttt ccgggaggcg tagcgcgtga
    12541 tgaatacatc gatcaactgg atgctctggg gtatggcatg tcgggctctt tatatgcaaa
    12601 tccgcattat aacctgcttt ggagctattc cgaatccacc aatttttatc ccgattccat
    12661 tcagataccg ctgctgatga ttggtggctg gtacgatcac aacaccgttg taatgctgga
    12721 tttttttgat gccatacgta catcatcgcc ggcagcggta cgcgacaagc acaaactgct
    12781 aatgggaccc tgggttcatg gtggtcacgg cgctgcatat gtgggtactg attcggcagg
    12841 acaactctcc taccctatgg cggccggaat ttcggattcg ctcgccatgc gctttctgaa
    12901 attttatttg ctcggagaag caaacggata cgacacactc agcaccatta ctaattttca
    12961 aatgggggtg aacacctggc aatacatcga aaactggcca cagagcggaa ttacagagac
    13021 caatctgtat ctgcacgaag acctgtcgct tcagccggtt tctccatcag gatggttcgg
    13081 aaacagaggg ttcagttacg atccacgcga tccatcgcca acggtaggtg gtccgacatt
    13141 acgtgccgac atggttcagg gtccatacga tcagtctcca acagttgaaa gccggggcga
    13201 tattcttgtt tttactacac cggaactcga gtctgatgca acggtacaag gctatccgaa
    13261 ggtagtgctt tacgtaaaat ccgattgcag ggacactgat tttgcagcac ggctgaccga
    13321 tgtttaccca gatggccgct cgatgctgct ggtggatggc atacgcagag cccgtttcag
    13381 agaaggttat acaacgagtg acaccatttt catgaacgac agctccaccg tttatcaggt
    13441 cgaaatagaa ctcaatccga catcgcacac ttttctggca ggacatagga tccggctcga
    13501 catcacgtct tcgaactatc cacgcttcaa caataatatg aatgacggcg gcccgatgta
    13561 tgtggccggt gatacaacag tagcgcacaa ccgggttttg tgcaatggca catcaccttc
    13621 aaagcttgtt ttaccggtgg atgttattga tgttggtgtt gaaaaaaaca gaaaacctga
    13681 actttcaata tttccgaatc cttgttccaa tgagctgttt gtaacaattc cggatggaat
    13741 tataaacaat attgttgttg tttcagttta tgatgcaact ggtaaaaaaa tcagtgcaaa
    13801 atcagacatc gagggaaata ttgtgaaaat aaatacttca tcgctggcgg cgggacttta
    13861 tacaattcag ctgcaaagcg atgttcaatg ttttactgtt acttttttca aaaaataatg
    13921 aaatgatata caataagcga taaaaagact acgtttatat taacaaatta attttttatg
    13981 agaagcacca tttttatttt tctgttattg cttgccggca tcgttgatgc acaaacaggc
    14041 gagtattatt tcatcgtcca tccgtttgaa tttgatgaaa tctacatgac tgctaaagca
    14101 cagcagacaa gacttgccag caaggtaaaa accatcacgg aaacaaccac gacttataaa
    14161 aaaggaaatc ctgaaaaagc cacgctaaaa acagtgttca ccctgaacca aaaaggtgag
    14221 accattcaga aagaagaata tgacgcaaaa ggtcaactga tgcgcaaaca cagcattatg
    14281 ctcaacgaca gcggctgggt ggaacaaaag aaaattgaag atggcaaggg gaaagtattg
    14341 tttcaatacg ataaaaaata tgatccgctg accggcaata tcatatcgta tgaataccgg
    14401 aaaaacgaca aactgaaatt gaaatacacc aaatcttatt gcggaaagaa tcttgcggaa
    14461 caaattgtct ataaaaaaga cggtgaaact gtttcaaggc gcttcgtata tacttataac
    14521 actgaatcga agttgcagat tacgcatttg tatgatggaa acggaaaact gttgcacacc
    14581 tggaatcatg attgtttgcc ggaaggcgaa ctgatggctg cacgcaaaga tacaaccaca
    14641 gtttgcaaaa tgcgcgaaga atctgctgac ggccattttg tgaattatac taaatcggtc
    14701 aatgaaaagg gacgactgac taccacaacc acttggtttc gtgctgaata taaggcagat
    14761 tcagtaaagg tggtgactga agggaaacat ccttcaacac gcttttatcg ctatggcgcc
    14821 gatggaaaac aatttgaaag cattgtcatg aatgatgaaa acatcattac tcataaatat
    14881 ttccagctta tgagtgaaaa tggcaatcaa ctgaaatatg aaaactggag ttactcgaag
    14941 aaacacggac tccgatgcac caataaagct atttctgaag aaaatgcaca ggggctgctg
    15001 acaaaaaaga cctattattg gtatggaaaa ttaaagagcg aaacaagttt ctcttataca
    15061 tatttctgat ttcagaaatt tcgcaatctc tgcagaacat cagctctggc tcaattccgg
    15121 agttgctgtt tttttttatt gactcgattt tttattcttt ataccatttt cccgggatct
    15181 acccactcat caaattgttc cggtgttaca agccctgatt ctatagcggc ttcgcgcaag
    15241 gtttttcctt ccttgtgcgc tttctttgca atgatggctg cattgtcgta gccgatatgc
    15301 gtgtttaacg cggtcacaag catcagcgaa ctttcaaggt tttttctgat agctgcaaga
    15361 ttaggctcaa tgccggctac acagttatca gtaaacgaat ggcatgcatc gccgatgagt
    15421 cgtgcagcaa aaagaaaatt ataaatcatc acgggcataa agacattcag ctgaaaatgg
    15481 ccgcccatgc cgcctgcagt gatagctgca tcgcagccga tcacctgtgc acacaccatc
    15541 gtcaccgctt cgggctgagt aggattgact ttgccgggca taatggaaga accgggttcg
    15601 ttttcaggaa gtagaatttc tccgataccg catcgcgggc cgcttgccag aagcctgatg
    15661 ttattggcaa tgtgcatcat actcactgca acggtcttca atgctccact ggcttccaca
    15721 atggcatcgt gcgccgagag tgattcaaat ttattttctg cagtaatgaa aggcagtttt
    15781 gtgagctctg caatttttgc cgccaccttt acatcatatc ctgcagtcgc attcagacct
    15841 gttcctacag cggtgccgcc caaaggtaat tcactcagat gcatcaatga attttcgatg
    15901 gctcgtattc catgatcaag ctgtgacaca taacccgaaa attcctgacc aagagtaagt
    15961 ggcgtagcgt ccatcaggtg cgtacggccg gttttcacaa tggctttcca tgcttcgctt
    16021 ttggcttgca gtgcgtcgcg cagcttttca agcgccggta tggtatgcca caccagcatg
    16081 gaatacgctg caatatgcat ggccgtcggg aaagtgtcat tgctgctctg cgacttgttt
    16141 acatcgtcgt tcggatgaag cgtttttgtt tcatccgtca gctttccgcc ggcaatcaca
    16201 tgtgcacgat tagcaatgac ttcgttgaca ttcatattgc tttgtgtgcc ggatccggtc
    16261 tgccaaacca cgagcggaaa ctgatcattc agcttgccca tggatatttc gttgcagacc
    16321 tctgctataa gctttgcttt atcagctgac aattttccga ggtcattgtt taccaatgcc
    16381 gctgcttttt tcaatatggc gaatgcttca ataatctcag ttggcatcag cgttccgcca
    16441 attctgaaat tgtctttcga acgttgggtt tgtgcgcccc agtatttgtc ggccggaaca
    16501 tccacaaggc ccatgctgtc tttttctttt ctgtattcca ttttctttat cgttacagtc
    16561 gttacgcgtt acaaagttac aaacgttact gaattcaaaa ctaatctctc ttcacacatt
    16621 ttcacatcat ttcgttacac gttacagtca ttacaaattt tcacaaatca tcacctactt
    16681 cctcgtccct ggagcccgaa gcttgtcacg tttcacgtga ggcatacggg ctaataatat
    16741 ttaactccgg agtttactcc gccaaggcgg aggagtatct gatggtgctg cgcagcttct
    16801 ttcacgcctt cggcacttct caaccttgac ctcgtcctca cacaccactc actgctcact
    16861 gctcactgct caccagtcac tgctcaccgc taaaacagtc gctccagatc atcaacagac
    16921 tcaacaatcg ctgccttatt gcctttcaca acaatcggcc tcttcaggag ttttggattt
    16981 tcggaaataa tctgcgccca ttcttcgccg gagaaatttt tccctttcag ctctttttta
    17041 taataatctt cctgcgtacg aacgatgtca atgatcggca cattcagctg ttgaacgagt
    17101 tttttaatgc aggccgcact gattcctgta ttcagataat caatgatgtc aatttcatcg
    17161 gtttttgatt tcacaagatc gagtcctgct ctgcttttgc gacaacgtgg attgtgatat
    17221 acggagattt tcatgtaaaa attttaattc gtgaaaatac aaaaagaatc catgcagccc
    17281 tatttctttt tccatttcag accgatttcc ataccaagca caaccgaggc catcgcgggg
    17341 tgacgatcta tgatatagtc gaatgtttta ggattcacat gatcggtact gccggtgtgt
    17401 ctgagaattt cattgctgaa aaccacaccg gcataaatgt tccaggttgt ctttcccgca
    17461 tcaaccgaca gtactaaata cggagttagc gtgaaatatg ctttttgcac cagtggttaa
    17521 caggaagtca aaataagaat gaaaagactc cttttcattt ttattgcttt cgtaaattca
    17581 gaggaagggt gtattgaaaa ccaaaaataa tgtttgctga ggtggggtgt tttttcagaa
    17641 cataacccca ggtgtaatcc tgataatgac tcagatcatt gtattccttt ttcacaagtg
    17701 atggaaaatt tgccccgata taaaaaccgt atggtgtttt tccgcttcgg ttctgatagg
    17761 tgaaaaccgg tgtcagaact gaaaaccact tattgtaaaa tgtaatacga tctgaataat
    17821 cgctgctgac aggataatca tcatgttcag gatcataaaa tgcaacctcg taatcgtaat
    17881 aataatacgg acaataaacc agctctgccc tcagcgaaaa tccccaggaa ttctttttgt
    17941 ttgaaaatat tgagggctgc acgctgaagc gatgaaaagc cgaatgcaca tcctgcgaaa
    18001 aatacgggcc gccaagcaaa ctcatcgggc tgatccattt ttcataaaaa cgggcattat
    18061 ttatctgaaa gccatatccg gccagacatt ccatataact cttcggccgt tggctgtacc
    18121 agatataacc accgctgaat cctccggtca gattggggac acccttatca tgaaaaccat
    18181 agtactcatc atttgaaagc cagccctgat agccaatcaa gcccgttgca tacactccgt
    18241 gatgttcgct gaacgaatgc atgatctgca aatcggtgtg attgaaataa taacccgtca
    18301 aattgacttt tgtctgatga atgctatcga aagtggcggt aacaggcggc tcgacaaaat
    18361 aagccttctg cacaatatag ggattgcagg atgcgaataa acatgcaagt ataacaatac
    18421 atacatattt catggccagt gttttggcaa atataaaaca aaaaagcgtg aaaaatactt
    18481 ttacgctttt ttgtaataaa atataattgt tttatatgcc aatcagcaac ctcaaccccg
    18541 gatagacgcc tttgaatttt ccggggaaat acatctgtgg agagaaatac cagaagtcgc
    18601 ccttgaagcc aaatcccagc ctgaaagtat ttttctcaaa cacatcaccc tgccggaaaa
    18661 cgaggtagcc catatcgatg ccggccatgg ttttgagccg cggaagaaaa ccttcttcaa
    18721 gtgcacttag attaataaag ctattaatag atcgggtacc gttttcggca aaggaaaaat
    18781 tccagtcgta gctgagatag tagccgatat cgtagtcgaa attgttccaa atataaccca
    18841 gtttgacttc tgctgacaac gaatacgggg ctttagagag acataaacct gagctcaaat
    18901 taagtacaat tttctcgtcc atctttttcc tggaataaat tatataaggt tttttatcag
    18961 cgcgaaatgt gctgtctgag taatcaatat ttgttgctct gacctcagat gcacgatgcc
    19021 agtccttttg gcttttcaaa gcaatcacat ctgtgataag cgccttaagc ataggactta
    19081 aaagaaccga atcattgtag aagcggatgt ttataaatcc tccaggtata tcgattctgc
    19141 attcgttcaa ttcaggatat ggaattattg attgcctttt ctcactataa acagtttttt
    19201 tcgtagaatc gagttgttcc atgacaagaa aacccggatt ataatatctt aatttaaaac
    19261 gctgtgtttt atcatagtaa ttgctttttg tagaataatg ctgttgcaca ctatctaata
    19321 acaaactcat ttgcatgttc ggatcagcaa ggaaaccaag gtcataacat ttaattgtta
    19381 tccatgcatt acgcgaaaaa atgtataatg agtctttcca gtactcttcg ccaagatagc
    19441 gaaactgttt ccatttgctc acatccagtt tttgtgttga accggcaacc tgagcctgca
    19501 ctgcagcggc aaagaacagc aaacataatc cgaacaatat ttttttcatg acaatttgtt
    19561 tttaatggaa caagggtgat tttatgttat tgcgcgctga taaccggaat tgtaaaagtt
    19621 tgtttttttc tgtcataacg aacatcggct gtagcagtat tgtttggaaa cgctggcttg
    19681 gtgcgccaga acaatatgcc ccgcacttgt ttctgaggcg tgtattcaat tggaaaaaca
    19741 tcattgacct gcgggtttcc gcctgcaatc aagggttcag aggtatgttg cttttcaata
    19801 aatatggtgt catgcgaagt tgagtaaact acatctgtct tttgcagaat aatagtatcg
    19861 tgtacgattg cagaagtcag aaaacgatct aggtaaatgg tctgaaaaac cacattctta
    19921 gctggtttca caagcgagtc caaactgttt gcagcatttc tgatttcttt tttttcagcc
    19981 attttttttg cagaatgttc tttctgtatc cacataaaat aagcggtggt cagcagcaac
    20041 acaaggattg cagcagccgc tactcgaaac agcactgccg tgagcgaaaa cattttcggc
    20101 ggattggctt tctgccatac acgtccgcgt gcagcctctg ctgcggcatc gtcattggca
    20161 gcttcttcaa gcaaagcctg acggattata ttatcaaact cagacgtttt catgactgac
    20221 attatttgta attcgttgaa taataaaact gcgggccttg cacagttgcg agcgcgaagt
    20281 agtttcgctg ataccgatca gttgtgcaat ttctttatgc gaaaatcctt cgatggcata
    20341 cagattaaat actgtgcggt atccggcagg catggtggta agaacctgct taatctgttc
    20401 tgcatcaaaa acgcttgcat caaagctttc ggacgcataa tcatcataca gcgccatatc
    20461 ttcttcgagt accagcaatc gtcgacgttt gagaaaacgc aacgcttcgt tgatgaccaa
    20521 tgtgtgaatc catcgtccca gactgccttc ggtacgaaca tcaaatgtat gcaatccgtt
    20581 gaaaacgcgg ataaaagcat cggccacgac atcttcggca tcgtgatgat tgcccagata
    20641 ccgcactgca atgcggaatg atgtgcggta atgcgcatca aacaattcgc gctgcgccga
    20701 ccggctgccg cttttgcatt gtctgatgag tttatcttcg ttcacggatg gttgattttg
    20761 ttgcaaatgt agtaacccgg caaatggaaa aaacgctgcc tgaagaacaa aaaatgttat
    20821 actatttttt cgccagagac ttcttcctga tcttcatgtt gaccaactca acaatcagcg
    20881 agaagaaaac agcaaaataa atgtaccctt tcgggacctc gatgtggaca ccttcgagca
    20941 tcagcatgaa tccgatcaga ataaggaatg acaatgcaag aatttcgagt gttggatttt
    21001 ttgcaataaa acgcgaaatg gatccggaga aagcaatcat cacaaacatg gaaataacaa
    21061 tggccgagat catgatcaac agctggtttg acaatccaat ggctgtaaga atggaatcaa
    21121 atgaaaatac aatattcagc aacacaatct gcaacaccac attgaaaaac tttttcccga
    21181 tgtccttttt tgtttggaca ggacccgttt ccattcttcc atgaatttct gaaacgctct
    21241 tggcaacaag gaaaagacct ccggcagcga gaataatttc acgaaaactg aaccccatct
    21301 cattaacgta aaacaacggc tcggtcattt gacgcatcag atacaccagc gcaatcaata
    21361 atgcgacacg cagaaacatg gcaaccacca aaccaatacg ttgcgcaggc cgctgctgat
    21421 ctgccggaag cgagttgctt acaatcgaga taaacaatat attgtcaaca cccaaaacga
    21481 tttcaagcaa cgtaagtgtc agcaatgata tccatgcttc gggagtgagg aagatttcca
    21541 tgtatgaaga atttctacaa aaatacgaat tctttgaacc cgatttattt accgaaaact
    21601 gattttaaat tgcacataaa catctaattt ctatttttac ataaaaaagt at
//
LOCUS       DAOPKE010000048        21594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2585_length_21594_cov_9.806119, whole genome shotgun sequence.
ACCESSION   DAOPKE010000048 DAOPKE010000000
VERSION     DAOPKE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_21594_cov_9.806119"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..486
                     /locus_tag="PKY63_07530"
     CDS             70..486
                     /locus_tag="PKY63_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00500.1"
                     /translation="MAKFVKNKVLEIGDQLQILKSEKYGYGDYGLVRMDKIPSLNFQN
                     QGGFTKYFSDKAKSTQKNQTINIENNYGQVIHAGQKSIITDKTNKASTEIKSNNQEQN
                     KSWFKKIITSNWTITIIGGLFVLIVGTFLLHKFGIL"
     gene            701..1198
                     /locus_tag="PKY63_07535"
     CDS             701..1198
                     /locus_tag="PKY63_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPF00501.1"
                     /translation="MVKSGRQFKSDPRVDEISFFIAQNDIEKAKQLLSETGIDTCDGY
                     LRTALIWAAFHNNIALLNWLIEKGANINHQDRNGYSALHFVAQEKRFDCAKILIEKGA
                     DLELLDNHDNTPLWTAIFNSRGENQIVELFIKHGANPDHINNHQQTPRKLAEMIQGFD
                     LTSLE"
     gene            1289..3364
                     /locus_tag="PKY63_07540"
     CDS             1289..3364
                     /locus_tag="PKY63_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPF00502.1"
                     /translation="MRAIFVLLAVVSMTVFSVAQTVEYTYRFSDYTIQNHGDFHTIVF
                     NGTKQYGVVGEPAMPYQQVSLLLPPGTEVASVEYVLENEITIPGNYMLPPLQPDIPYS
                     IGSTGTKIKSETVYSLNESYPQKYSSTIATQYLNGYSFALGKFTPVRWNPATGSLSYF
                     SNVRIIIHYAQSDNSAKAFKNISSSENAMLRTRTLAQNNDMMDRYPEKEVLDAYQMLI
                     ITTSALRPGFTPLKNYYDSIGINTRIATTDSIYSVMTGDGIRTRIRNYIIQEYQTNSI
                     EYVLLGGDVSKIGARGFYCYVVSGSGYEDDGIPADLYYSALDGNFDEDGDGIFGEALQ
                     DSVDLLPEVAIGRFPVETADELELLVNKTIQYQKYPIISDMNKVVLLGENLLDTPLTM
                     GQDYLALLIDNHNDNGYTTSGIPSATNFIDTIYDQWNIAGGFPSYEWDMPELTTAINA
                     GSSFIYHVGHSAELYMMRMSDMELDNTTFSSLNGTDHSYGLLYSHGCMCGSFDWNDCI
                     AELSLLLENYLVACVVNSRYGWFNEGQTEGPSQHLNREFVNAIYNPSIKERRIGQAFM
                     MSKIATAPWVDLAGEFEFGAQRWVHYDNNLLGDPALRMWIDAIEVGIEASQNSEIEFY
                     PNPSDGHIVVDERFVGAQLSVFNAIGGLVYNGIINNQNIDLTLFSAGMYLLQINNGLE
                     IFGGKVLIR"
     gene            3699..5132
                     /locus_tag="PKY63_07545"
     CDS             3699..5132
                     /locus_tag="PKY63_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF00503.1"
                     /translation="MKEKKEKAPAVKTIETAARNIQSENIVLREHAEFHNCYSVKSVQ
                     DDIRFLQVVYGKPISIKPEDVTIDFVNNLKVIGKDIYFFFKLLETDMNPHNYIKSKYE
                     FIRRRIPNGDPNLFYIAWNGLIKNDSFRRLLIQTFSLSRFSDILCPQSIATPGASMTR
                     YRTKLVLADDMATTPDGKRRTTGRALEDGSGLMMNEEIVWLPNDKAIPLFPQAYVPLN
                     QTHVIMIRHGKSEHESGGDNPEFVGSGFWDTWSNNRRISGSMSNFLSDGGKKTAQELG
                     LDFKVAVDILDKAGYPMWSWSKDNPVQVFGSESENTEQTARIFLQNAGYTNMQFHAMF
                     GLNSQKYGALTFKYKNEIIDQLARIHSGGDEPTPETKTKVSKIFKNRFYHYPEGETLI
                     EADWRIAYSFIDLLNLNLGKRILLADHSGAIRVFEAVIRTLDFAEYSTIKEAQDSIMA
                     MCYQPGKNLRYDYLQRKDFPLRKRDKK"
     gene            5138..5668
                     /locus_tag="PKY63_07550"
     CDS             5138..5668
                     /locus_tag="PKY63_07550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPF00504.1"
                     /translation="MSTPHVILIAAGSTIIGIAAVLLIIKPRISYLRRRLFTDPTTGL
                     LNYEGFLNYLKRYQKKESFVLFLLDLDDFRRFNRQSYDTGDVVLKLFATRLSKKMNGI
                     AICARYRLGDEFLLVAQEKDTEKVSSLLANLNTEVSYKDDPVHFSSGIAAIECNDASC
                     FNAIRTAHDILMSKKI"
     gene            complement(5691..6731)
                     /gene="selD"
                     /locus_tag="PKY63_07555"
     CDS             complement(5691..6731)
                     /gene="selD"
                     /locus_tag="PKY63_07555"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPF00505.1"
                     /translation="MNGENKIRLTQFSHGAGCGCKISPNVLCTILESQIETTADARLL
                     VGHDSCDDAAVYDLGDGTAVISTTDFFMPIVDDPFDFGMIAATNAISDVYAMGGVPLV
                     AIAVLGWPVDKIDPEIARIVVDGGRQACKNAGILLAGGHSIDSPEPIFGLAVTGRVDI
                     KNLKQNNTAKAGCKLYLTKPLGIGILTTAEKQGKLRPEHIRTATESMIKLNSIGAEFA
                     KMPGITSMTDVTGFGLLGHLREMCEGSNVSAQIEYTKIPRFDSIEDYMIQKCFPGGTF
                     RNWESYGHHVKLENNDWKPLLCDPQTSGGLLIAVEPACCNEIEEMLKSHNMPSIPFGY
                     LTERTEMLIEVK"
     gene            complement(6715..7740)
                     /gene="mnmH"
                     /locus_tag="PKY63_07560"
     CDS             complement(6715..7740)
                     /gene="mnmH"
                     /locus_tag="PKY63_07560"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPF00506.1"
                     /translation="MIQNISIHEYLSHPAPLIDVRSPGEFTQGHIPGAVNVPLFNNDE
                     RAEIGTVYLRRSRDEAMQLGFQFANPKKQWFIDEVRKVAPDGPVVIHCWRGGMRSRLF
                     AEHLLENGFASVSVITGGYKSWRRLLNEHWKKTHQLIILGGYTGSGKTPIIKILHDLG
                     HQAIDLEGLACHKGSAFGAIGQKPQPTTEQFENELFNIWRNYDQERPVWLEDESRNIG
                     HVQLPADLFAQMRDAVTFFLDIPRSVRAEYLVQDYAAASINDLKESILKIARRLGGQN
                     VNQALQWLDEGNLYEVAYISLSYYDKAYTRGLEFHDNKKVWMIASDSIDAEKNTELIL
                     KEYERRK"
     gene            7954..9927
                     /locus_tag="PKY63_07565"
     CDS             7954..9927
                     /locus_tag="PKY63_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPF00507.1"
                     /translation="MEENKDLNEGKVEETKQENPAKENEVDVTSLVGSNIKALLVDVG
                     TSMYRTIRYPVGQFFGPVDLGLHHSTKYNSMNIGTGLLSGSIFPGSNRLIFTGFSPCW
                     TGFYISSMGGAGLVFDNLGIDLLSITGRAASPSVLILNRVHGEEIQLRVEPLEIHDIW
                     RSGRQGVYSLMDYVNEKYRDEYSDAYRILATGPAAETTDFAGVVSVPVRKGELTYVDT
                     WAGRGGFGSKMFQEFNIVAIIYGGTFIDKDFRDRSVADKWFQDRYNKKLAAKDLEATT
                     KYRFDPKFETGGTFGVNFATMNGNLMAFNYDSFFWSEEDRKKVHKDFILDHYLKQFNE
                     ETIKQKQNWTCGEPCVAVCKKMNVEYKKDYEPYQTMGPQCGIFDQRAAELLNHHADML
                     GFDAISVGGVLSWLMELLARGLLTKEDLQVTRYPIWHRERFDVVKDSMHNARLGVEIL
                     DTIVQKRGIIDFQLGPRRWGRKLARERGREILDSFLYLAFGRRGWIVPNQYWVPGVLS
                     PMPIVGKYYMYYGTDFMHPRVLGHTNTTRFQKELIMDNLGLCRFHRNWAEDFMPDIIG
                     QLYGEEIKDKFTESLKSIAYNINSRNNSTLWESWRNVELLETYLKRKHEEEGNNDPDL
                     VGWIDYFKRDRRTAAIDYWYEVHKGICESMNEF"
     gene            10748..11239
                     /locus_tag="PKY63_07570"
     CDS             10748..11239
                     /locus_tag="PKY63_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF00508.1"
                     /translation="MIDRIQQLMKTYELTAADLADTLGTERSGISHFLSGRNKPSLAF
                     ITKLLEKFPDVNPDWLLLGTGTMLRSNQNQVTPEIQAYAAPKPQDPVNQQNNTTPLPA
                     KQISVAEDEEPAEYHTKRPAEKPIQAEKKQTPSPVQIPAGTEIERIVIFYNDHSFSEY
                     KMR"
     gene            complement(11256..11678)
                     /gene="ndk"
                     /locus_tag="PKY63_07575"
     CDS             complement(11256..11678)
                     /gene="ndk"
                     /locus_tag="PKY63_07575"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPF00509.1"
                     /translation="MAGSITLAIIKPKAVANGHIGAILHTINAGGFRIRGMKMIQLTR
                     KQAEEFYAVHKKQDFFDGLIDFMTSGPIVVASISGANAVDDFRKLVGATDPKAASPDT
                     IRGKFGTNITMNAIHGSDSDDNAIRETNILFKPEELFD"
     gene            11752..12747
                     /locus_tag="PKY63_07580"
     CDS             11752..12747
                     /locus_tag="PKY63_07580"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPF00510.1"
                     /translation="MNTLDKNHICKFICTDPQNVVVVSHTSPDGDAIGSALAMQKTLT
                     AFGHHAVAILPDVYPDYYSWLPGVEDIVIAENEFDKALQLIANASLQVIVDMNALKRG
                     QIIEKALEQADAKRILIDHHILTNNIFDAAYSTPDVSSTSELVYNVIEALNPALITKD
                     VAECIYTGIVTDTANFFHGNMSPRTFDITANLMRKNIDIIKINQVLYHTFSENRIRLL
                     GFSISERLHLLSDFHAAYIYLNKEDLIRFNYSEGDSEDIVNYGLAIKGIHISAFFIEK
                     TDFIKISLRSEGDINVNAMASKYFNGGGHKNASGAHFYGSLTEAISLYEQALREL"
     gene            12744..13283
                     /locus_tag="PKY63_07585"
     CDS             12744..13283
                     /locus_tag="PKY63_07585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPF00511.1"
                     /translation="MIKSLFYFLCCFLLLASCGEKSNDNTGMLDPERYSKNLEKANNL
                     YTQSEDGQIDDFVSRNRWEMTKTGSGLRYMIYKTGQGRKVADKTVVRYHFRVNLINGS
                     LCDESAKTGPKEIWIGHADVVSGLEEGLLLLREGDKAKFIIPSHLAYGWIGDSKTIPS
                     RAVLVYDVEILQVRDYNVE"
     gene            13285..14163
                     /locus_tag="PKY63_07590"
     CDS             13285..14163
                     /locus_tag="PKY63_07590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPF00512.1"
                     /translation="MRNLFLCFLVALTAFSCSQSDYPGYDLSENGLNYRIVDDQDGLQ
                     VAVGDFINVRIQYKTMSDSVFFDSDQEIVPVWIPVNKPSFRGDIMEGLAMLSVGDSAS
                     FVVRADTFFRMTIGALSFPSFLEKDSMMYVNLRVLEVKTQSEFAQEKQILAQEVEKQF
                     AELKEKEMLDLKYYLKINNITQTPTESGLIFIPVKVGSGPRLITGQTVRCHYTGTLID
                     GQIFDSSVGKNPLEVVVGSPDLIDGFNEALTMMSVGSQATVIMPSKIGFGRSEIDSPI
                     PPYATVIFDITVVSAE"
     gene            14601..15188
                     /locus_tag="PKY63_07595"
     CDS             14601..15188
                     /locus_tag="PKY63_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00513.1"
                     /translation="MKKKLFYALFLGALTVTGLGFSSCSNSNPDSQNKTEDVNDVDVE
                     EEDLFVSKDGKFKANFPGSPTRDVQMVATEAGDIEMISFVYEKSATEASMIAYSDYPS
                     SLIDAQDPMSILKNARDGSMEGNIVESNDEMKVNGWPAIRTRAIDKTQNLHYVLEVVL
                     AKNRLYQILMVRDGSYPDDAAVEEFFDTFEITMKK"
     gene            complement(15247..15690)
                     /locus_tag="PKY63_07600"
     CDS             complement(15247..15690)
                     /locus_tag="PKY63_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00514.1"
                     /translation="MKNYSKIIMLLAISTGLFSCKCNKIVEKQQDTKQIQIIKTEFGE
                     KMATEKLSYTIDTVWVDGLTLHTILTYTGEKTDVSFAMAFNGAWLKSYPPKAYLNILP
                     SVAATEGKKQVKHHLVYDLTPLSSGNPYFEVYVTGYKQGFRIGGE"
     gene            15781..16923
                     /locus_tag="PKY63_07605"
     CDS             15781..16923
                     /locus_tag="PKY63_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF00515.1"
                     /translation="MKPHIVFLAAWYPDERDPMFGLFVHKHAVLMTKEFSVSVLHVTN
                     YSDKANGMVVYHKDGVSVHQLNIKTKNRFLRWLYFFVAGLQAYRYICKKEGQAVLNHV
                     HVLTRMGVLAVWIRFRRRIPYVITEHWSRYFPNPGTYRNVIRKWVTRFVCRKASAMSA
                     VSCGLKDAMIKHRLGVDKQWFIVNNAVNEDVFDLPTDKPNNVPIRFINVSCFEDRSKN
                     LSGLVKCVEMLVIEGLKIECVLVGSGEDFNEISSLIKSKKLEKQFIMTGQLNEMMVAG
                     QMKKCHFYVQSSHYENVPVVISEALMCGLPVVATNVGGLNEMIDDSNGYLVEHNSLTE
                     LSSAIRMMTENYSAYDRQKIRQAAMHKYSAVAVRQQMLQLYSALIK"
     gene            16920..18563
                     /locus_tag="PKY63_07610"
     CDS             16920..18563
                     /locus_tag="PKY63_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00516.1"
                     /translation="MIDKQEYISYAIMVNGQHLKRWQAEAIEILNQSGLCRCVLIISN
                     PDDTSHKKNVFGKLLNRRLLYNFLLNRYFNVGAEQMCTLPTDVPIIEIAAEQKGVSHY
                     FTEADILKIKEFRPAFIVRFGYNILRGAILEAAPYGIWSYHHGDERKFRGGPFGFHEI
                     RTKTPVSGVILQKLTSRLDAGQVLLRREYITVMHSWKEMRQRLLAANSDMVLQAVKMY
                     IINNEKIPAASTTQAPVFKAPGNMRMLWFIAALWMRRVSFHIKRLLVYESWHVESGLT
                     YNSPLVPVLPTVTNVSASNSGAFHADPFLLQTSDNLYVLSEFFSYSRKLGSITANRDE
                     QDGRLWLQKDTHLAYPYVFSYSGEHWIIPENADSGKCIAYRVDNELNIIEELVLLDLP
                     AVDPSLVFHEEKFYLFCGLKDQLPNEKLFVFWSDSLGGPYHAHAQNPVKVSPVGSRPG
                     GSMINWQGNLYRPAQVSDIYYGYKILIYKILKLSPQLFVEIEKSEINPAIFGSQYNGL
                     HTYSIYGNHYAVDLKTHRVGLAAFIFKWKQNRKNRRADA"
     gene            18556..19794
                     /locus_tag="PKY63_07615"
     CDS             18556..19794
                     /locus_tag="PKY63_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00517.1"
                     /translation="MLSNLLRTVGIRIATAVISLLIVVVSGQMLGAEVLGEISLFIVA
                     VTIINLVGGFAGGAALVYLIPRNRLLGLFVISQLWAGVSGLFTTCFLFLIGAFPQNLV
                     WHVMLLGIVGSLNQNNLSVLLANQRIKAHNLLTLLQSVLMIGFLMGFTFLYKIDTILA
                     YILSMYASYLPVLFLSWMLAGKGTQTGKVSPSQALKVLFGYGSLVQVSSILALLTYRL
                     TYYYTEAWLGFAALGLLSVAAQISEAVWILPKSIALVQFSKISNLKNDADSAHLTLFL
                     GVITSVFTFLALSVLVFLPDQVYTFIFGKDFYGVGAVVRIFAPGIFAMSLNIILSHFF
                     SGTGKIIFNLVGSAIGLLTVAICGSIFIKNGNISHAAMVSTFGYAVNFIFAWLCFWWI
                     TRRNSLSFRKGLLVFRGVKK"
     gene            complement(19848..20306)
                     /locus_tag="PKY63_07620"
     CDS             complement(19848..20306)
                     /locus_tag="PKY63_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00518.1"
                     /translation="MKQFPYLFIILLVCLPLISWSQSARFEGLTSYQINLDSNSAIVA
                     ADKIINIHSKTTGKLSIQLFLCENKYEGGSLKGIPCGEVKVSNGLKKGEKVMDFSADI
                     SFTRPLRGSFYPVVLLLENVKGENLIIDYVTFDMFTINKKSVGILTGKNN"
     gene            complement(20336..21415)
                     /locus_tag="PKY63_07625"
     CDS             complement(20336..21415)
                     /locus_tag="PKY63_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPF00519.1"
                     /translation="MTRLLASLIFVLILISSQNTFCQNNDKRSDVYFTEDISPEGVMK
                     LFTFMKDSVHGNVGIKVHFGEDGNAYYLPATLVEPLCKELNATLIETNVAYKGRRHET
                     GTHIELAKEHGFTFAPIDILDSAGTLMLPIEGGTWFDSARIGKNIEKYETIIYFTHFK
                     GHGSAGFGGCIKNVSMGMATIEGKKLMHFNNFPKTDSTKCIGCGACVADCPVGVITLN
                     PLNIDKAKCIGCGKCITSCHVAAISNPTDTDKTRRFLEGLVEYAKPVCELKTSIYFNV
                     IINVSPSCDCARNPKKPFVADIGILASFDIVAIEAAAHDLVDKAHKCDDAFLKENSVS
                     GKHQIAYAAKMSMGNIKYRLINIDK"
BASE COUNT         6258 a         4155 c         4757 g         6424 t
ORIGIN      
        1 atattttaaa ctgctcttaa tcttgctttt aggcggtttt attcatagga gttcggtatt
       61 gacagagaat tggctaaatt cgtaaaaaat aaagttcttg agattggtga ccaattacaa
      121 atattaaaat cagaaaaata tggctatgga gattatggat tagtacgaat ggataaaatt
      181 ccatctttaa attttcaaaa ccaagggggc tttacaaagt acttttctga caaggccaaa
      241 tctacccaaa aaaatcagac aataaatatt gagaataatt atgggcaagt tattcatgct
      301 ggacaaaaat caattatcac tgataaaacg aataaagcat caactgaaat taaatcgaat
      361 aatcaagaac agaataaatc gtggttcaag aaaatcatta caagtaattg gacaattaca
      421 ataatcggag gattattcgt attaatagtt ggaactttct tgttacataa atttggaata
      481 ttatgaagag tgaagctttt atctttgttt aggaaatcag tgctggttga aagttttgtg
      541 caccaaaagc cgctaatacg caaagcccga acacgataac atcattatca aattcatatt
      601 atgaacacca agcacaaaca ccatcaaact agaattagcg ctcaagaacc acaaaacaaa
      661 gtagtttcat tcaaatctat ctcagttcca aatacactac atggtcaaat caggcaggca
      721 atttaaatcg gatcctcgtg ttgacgaaat atcatttttc attgcgcaaa atgacattga
      781 aaaagccaag cagctattgt cagaaacagg cattgacacc tgtgatggct atttaagaac
      841 agctttgatc tgggcagcgt ttcacaataa cattgctctt ttaaactggc taatcgaaaa
      901 gggcgcaaat attaatcacc aggaccgaaa tgggtattca gcccttcatt ttgttgctca
      961 ggagaagcgc tttgattgtg caaaaatact tattgaaaaa ggtgctgatc ttgaactttt
     1021 agataatcac gataacacac ctctttggac agcaattttc aattcacggg gtgaaaatca
     1081 aatagttgag ttatttataa agcatggtgc taaccccgat catattaaca atcatcagca
     1141 gactccaaga aaactggctg aaatgattca gggctttgac ctgacatctc ttgaatagta
     1201 gctattatac ctgctattgt cattggattt ttctcccccc atgcaaccaa tttcccctaa
     1261 atttgcactg ataatcaaag acttttttat gcgcgcaatt tttgttctgc tggcagttgt
     1321 ttcgatgact gttttttctg ttgcccagac cgttgaatac acctatcgtt tcagcgatta
     1381 taccatacag aatcatggtg attttcacac aatcgttttc aatggaacaa agcaatacgg
     1441 cgtggtaggc gagccagcaa tgccgtatca gcaggtatcg ctgttgctgc cgccgggcac
     1501 tgaagtagct tcggttgaat atgtacttga aaacgaaatt actattcccg gaaactacat
     1561 gctgcctccg ttgcagcccg atatacctta cagcattggc agtacaggaa caaaaatcaa
     1621 atcagagaca gtgtattcgc tcaacgaatc ctatccgcaa aagtacagca gcacgattgc
     1681 aacgcaatat ctgaacggct attcgtttgc actgggcaaa ttcactccgg tgcgctggaa
     1741 ccctgcaact ggctccctgt cttatttttc aaacgtcaga attattattc attatgcaca
     1801 gtcagacaat tcagcaaaag cgtttaagaa tataagctct tctgaaaatg caatgctgcg
     1861 gactcgcaca cttgcacaga acaatgacat gatggatcgc tatccagaaa aagaagtgct
     1921 ggatgcctat caaatgctga taattactac atcggcgctg cgacccggat ttactccatt
     1981 gaaaaattat tacgacagta ttggcatcaa cacacgcata gctaccaccg acagcattta
     2041 ttctgttatg acaggtgatg ggattcgtac ccgtattcgt aattatatta ttcaggaata
     2101 tcagaccaac tccattgaat atgttttgct gggaggcgac gtaagcaaga ttggtgcccg
     2161 tgggttttac tgttatgttg tttctggttc aggatacgaa gacgatggaa ttccggccga
     2221 cttatattat tcggcactgg atggcaactt tgatgaagat ggagatggca tctttggtga
     2281 ggccttacaa gattcggttg atttgctgcc cgaagtagct attggtcgtt tcccggttga
     2341 aactgcagat gaactagaat tattggtaaa caaaaccatt caataccaaa aatatccgat
     2401 aatctccgac atgaataaag ttgttttgct gggcgaaaac ctgcttgata caccattgac
     2461 catggggcag gattatctgg cattgctgat tgataatcac aacgacaatg gatatacaac
     2521 cagtggaata ccatcggcaa caaatttcat tgataccata tatgatcaat ggaatatagc
     2581 cggtggattt ccttcttatg aatgggatat gcccgaattg acaacagcta tcaatgccgg
     2641 atcatcgttt atctatcatg tcgggcattc ggccgagttg tatatgatgc gcatgagtga
     2701 catggagtta gacaatacaa cattttcatc attaaacgga accgatcatt cctacggact
     2761 gctttattcg cacggctgca tgtgtggctc gtttgattgg aatgattgca ttgccgaatt
     2821 gtcgttgttg cttgaaaatt atctggtagc ttgtgttgta aattcgcggt atggatggtt
     2881 caacgaagga caaaccgaag gtccgtcgca gcatctgaac agggaatttg ttaatgccat
     2941 ttataatcca tccattaagg agcggcgtat tggacaggca tttatgatgt cgaaaattgc
     3001 tactgcaccc tgggttgatc ttgctggtga atttgaattc ggtgcacagc gctgggtgca
     3061 ttacgacaat aatctgttgg gcgatccggc tctgcgaatg tggattgatg ctattgaagt
     3121 aggaatcgaa gcttctcaaa acagcgaaat tgaattttat ccaaatcctt cggatggaca
     3181 tattgttgtt gacgaaaggt ttgttggcgc gcagcttagt gttttcaatg ccatcggcgg
     3241 gctggtgtac aacggaataa taaataatca gaacatagat ctgacgctgt tttcagcagg
     3301 tatgtatctg ctgcagatca acaatggtct ggagattttc ggtggaaaag tgttgattcg
     3361 ttaaaaacgg ctcaaagctc aacgtcccgc cttcagcgtg ataaactcca ggcacaatgc
     3421 ttttgaagtc aacctcttct tgctgacggt caaatgtcgg ggggcgtcca atattcccat
     3481 ttgccaacac aaatgacaat tcgttgaata atgattcgtt ttgtacaaac aaagtggaaa
     3541 aacaaaccac ttcgtaaaaa agtcaggaaa agcatttttt tcacaacgga atattagtat
     3601 ctttgtgact cttttgctgt aaatttcgca tattattgat tatcagtatt ttagaaaagt
     3661 gaaaagaatc aacaaaatga acggaaaaaa aagatgatat gaaagaaaaa aaagaaaaag
     3721 cacctgcggt aaaaacaatt gaaacagctg caagaaacat tcagagcgaa aacatagtac
     3781 ttcgcgaaca tgccgaattt cacaactgct attcggttaa gagtgtacaa gacgacattc
     3841 gctttcttca ggtagtatat ggcaaaccca tttcaattaa gcccgaagac gtaaccattg
     3901 attttgtcaa caatttgaaa gtcatcggaa aagatattta tttctttttc aaattgctgg
     3961 agaccgacat gaatccgcat aattacatta aatcaaaata tgaattcatc cggcgtcgca
     4021 ttcccaatgg cgatccgaat ctgttttata tagcgtggaa cggattgatt aaaaacgaca
     4081 gtttcaggcg tttgcttatt caaacattca gtctgagtcg attcagtgat attttatgtc
     4141 ctcaaagcat tgccactccc ggtgcatcga tgacccgtta tcgtacaaag cttgttttgg
     4201 ccgatgatat ggcaacaacg cctgatggta agcgtcgcac aaccggccgg gctcttgaag
     4261 atggcagtgg attgatgatg aacgaagaaa tagtctggtt gcccaatgat aaagccattc
     4321 ctttgtttcc gcaggcatac gttccattga atcagacaca tgtgattatg atacgtcatg
     4381 gcaagtctga acatgagtct ggcggcgaca atccggagtt tgtcggttcg ggattctggg
     4441 atacatggag caacaatcgc cgcatcagtg gttcgatgag taatttttta agcgatggag
     4501 gaaagaaaac ggcacaggag ttaggacttg attttaaggt agccgttgat attttagaca
     4561 aagcaggata tcccatgtgg tcgtggtcga aagacaatcc ggtacaggta ttcggaagtg
     4621 aatcggaaaa cacggaacaa accgcacgga tatttctgca gaatgccggc tatacaaaca
     4681 tgcagtttca cgccatgttt ggtttaaatt cgcagaaata cggagcattg acgttcaagt
     4741 ataagaatga aattattgat cagctggcac ggattcattc gggtggagat gagccaacac
     4801 cggaaacaaa gacgaaagtt tcgaaaatat tcaagaatcg tttttatcat tatcccgaag
     4861 gtgaaacact gattgaagct gattggagaa ttgcctattc ttttattgat ttattgaatc
     4921 tgaatctggg aaaacgcatt ttgctggcag atcattcggg cgccatacgc gttttcgaag
     4981 cagtgatacg tacgcttgat tttgctgaat acagcaccat caaagaagca caggacagca
     5041 tcatggctat gtgttatcag ccaggtaaaa accttcgtta cgattatctg cagcgaaaag
     5101 actttcctct gcgcaaacgc gataagaaat agaattcatg agtacaccgc acgttatatt
     5161 aattgcagca ggaagcacca ttatcggtat tgccgcagtc cttcttatca ttaaaccccg
     5221 aatcagttat ctgcggcggc gtctgtttac agatccgaca actggattgt tgaattatga
     5281 agggtttttg aattatctga aacgatatca gaaaaaagaa tcttttgttt tgtttcttct
     5341 cgatctcgat gatttcagac gttttaatcg tcagagttac gacactggcg atgttgttct
     5401 gaaattgttt gctacgcgtc tttcaaaaaa aatgaatgga attgctattt gtgcacggta
     5461 tcgtctgggt gatgaattct tgcttgtagc tcaggaaaag gataccgaaa aagtatcatc
     5521 gttgcttgcc aatcttaata ctgaagttag ttacaaggat gatcctgtgc atttttcatc
     5581 tggtattgct gctatcgaat gcaacgatgc cagttgtttc aatgccatca gaacagcgca
     5641 tgatattctg atgagtaaaa agatataaga ttgtttttct ctttgaagta ttatttcact
     5701 tctatcagca tttctgttct ttccgtcaga taaccaaatg gaatcgaagg catattgtgc
     5761 gatttcagca tttcttcaat ttcgttgcag catgcaggtt caacggcaat caacaatcct
     5821 ccgctggttt gtggatcgca cagaagtggc ttccagtcat tattttcaag ctttacatgg
     5881 tgtccgtagc tttcccagtt tctaaatgtt ccgccaggaa agcatttctg aatcatatag
     5941 tcttcgatgg aatcgaagcg tggaatttta gtgtattcaa tttgagccga cacattgctg
     6001 ccttcgcaca tctcgcgcaa atggccaagc aagccaaatc cggtaacatc ggtcatggat
     6061 gtaatgcctg gcatttttgc aaattcagcc ccgatgctgt tgagttttat catcgattct
     6121 gtggcagtac gtatatgttc cggtcgcagt tttccttgct tttcagcggt tgtcagaatt
     6181 ccgataccca agggttttgt tagatataat ttgcacccgg ctttggccgt attgttttgt
     6241 ttcagatttt taatatcgac gcgacccgtt acagccagac cgaaaatggg ttcaggacta
     6301 tcaatgctat gaccgccggc cagcagaatg ccagcatttt tgcacgcctg acgtccgcca
     6361 tccactacaa tacgggcaat ttcaggatca attttatcga ccggccagcc cagtacagca
     6421 atggcaacga gtggaactcc acccatggca tacacatcgc tgatggcatt ggtggcggca
     6481 atcattccga aatcgaatgg atcatcgaca atgggcataa aaaaatcggt ggtgctgatg
     6541 acagcagtgc catcgcccag atcataaacc gccgcgtcgt cgcaactgtc atgaccaacc
     6601 agcaggcgcg catcagcagt agtttcaatc tgactttcga gaatagtaca caatacatta
     6661 ggcgaaatct tacatccgca acccgcacca tgactgaatt gtgtgagtcg tattttattt
     6721 tctccgttca tattccttta aaattaattc tgtgtttttt tcagcatcga tgctgtcgga
     6781 tgcaatcatc caaacctttt tgttatcatg aaattcaagt ccgcgcgtat aggccttgtc
     6841 gtaatacgaa agtgaaatgt aagccacttc gtacagattt ccttcatcga gccattgcag
     6901 cgcctgattg acattttgtc cgcccagccg gcgtgcaatt tttaaaattg attccttcag
     6961 atcattgatg ctggctgcgg catagtcctg gacaagatat tccgcacgga cgcttcgagg
     7021 aatgtcaaga aaaaaagtaa ctgcatcccg catctgtgca aataaatcgg cgggcagctg
     7081 cacatgaccg atgttgcggc tttcatcttc gagccataca ggtcgctcct gatcatagtt
     7141 tcgccagata ttgaacaatt cgttttcgaa ttgctcggtt gttggctgcg gtttttgtcc
     7201 gatggcacca aatgccgatc ccttgtgaca ggccaaccct tcgagatcaa ttgcctgatg
     7261 gccgagatcg tgaagtattt taataatcgg cgtttttcca ctgccggtat aaccacccag
     7321 aataatcagc tgatgagttt ttttccagtg ctcattcagc aaacggcgcc acgacttgta
     7381 tccacctgta ataactgaaa cactggcaaa accattttcg agtagatgct ccgcaaacag
     7441 ccggctgcgc ataccgccgc gccagcagtg aatcacaaca ggaccatcgg gagccacttt
     7501 gcgcacttcg tcgataaacc attgcttttt aggattggca aactgaaatc ccaactgcat
     7561 ggcttcgtcg cgggagcggc gcaaatacac ggttccaatc tctgcccgct catcattatt
     7621 gaacagaggc acattgactg cacccggaat gtgaccctgt gtaaattcgc ctggcgagcg
     7681 cacatcgatg agtggggcag gatgagacaa atattcgtgt atggagatgt tttgaatcac
     7741 gatgtaaaag tagttgattt ctttgagacg ctggataagg ttgaggataa ggtcaaggtt
     7801 gaggtcaagg ttgaggttga ggtcaagttt gaggtcacaa tttaaggtaa ggttgtgaaa
     7861 tcatagcacg ttatgcagca attcacatat ccaaatcctt gacttagcct gatttttcac
     7921 tatctttgct atcttttgca aaaacgaaat acgatggaag aaaacaaaga tttgaacgaa
     7981 gggaaagttg aagaaacaaa acaggaaaat cccgctaaag aaaacgaagt agatgttaca
     8041 tcgcttgttg gatccaatat aaaagcattg ttggtcgatg taggcacttc gatgtaccgc
     8101 accatacgct atccggtagg acagtttttc gggccggtcg atctgggatt gcatcattcc
     8161 accaaataca attcgatgaa cattggaacc ggattattgt ccggatctat ttttccagga
     8221 tcaaaccgat tgatctttac cggcttcagt ccatgctgga ccggatttta tatttcgagt
     8281 atgggcggag ccggactggt tttcgacaat cttggaatcg atttgctcag cataacaggc
     8341 cgtgcagcat caccatcggt gctgatactt aatcgtgtac acggagaaga aattcaatta
     8401 cgtgttgagc ccctcgaaat tcacgatatc tggcgttcag gccggcaagg ggtgtattcg
     8461 ctgatggatt atgtgaacga aaaataccgc gacgaataca gcgatgccta ccgcatattg
     8521 gctacgggac cagccgccga aacaaccgat tttgccggag tggtcagcgt accggtacga
     8581 aaaggagaac tcacctatgt cgatacctgg gcagggcggg gtggatttgg ctcaaaaatg
     8641 tttcaggaat ttaatattgt agctattatt tacggaggaa cttttattga taaggatttc
     8701 cgcgatcgca gcgtagctga caaatggttt caggaccgtt acaacaaaaa gctggcagcc
     8761 aaagaccttg aagccaccac caaatatcgt ttcgatccaa aatttgagac cggaggcact
     8821 tttggagtga atttcgccac gatgaacgga aatctgatgg cgtttaatta cgactcattt
     8881 ttctggtctg aagaagaccg gaaaaaagtc cacaaagatt ttattcttga tcattatctc
     8941 aaacaattca acgaagagac catcaagcaa aagcagaact ggacctgtgg tgagccctgc
     9001 gtggcggttt gcaagaaaat gaatgttgaa tataaaaaag attacgagcc ctatcagacc
     9061 atgggaccgc aatgtggcat cttcgatcaa cgcgccgcag agcttttgaa ccaccatgcc
     9121 gatatgcttg gattcgatgc tatttcggtc ggtggagtat tgtcgtggct gatggaattg
     9181 cttgcgcgcg ggttgctcac caaagaagac ctgcaggtaa cccgttatcc gatctggcac
     9241 agagagcgat tcgacgttgt aaaagattcg atgcataatg cccggctggg tgtcgaaata
     9301 ctggatacca ttgttcaaaa acgtggcata atcgactttc agttaggccc gcgtcgttgg
     9361 ggacgcaaac tggcgcgtga acgcggccgc gaaatactcg acagttttct ttatctcgct
     9421 tttggccgtc gtggctggat tgttcccaat caatattggg ttcccggcgt gttgtcgccg
     9481 atgcctatag ttggaaaata ttatatgtac tacggaacag acttcatgca tccacgggta
     9541 cttggacata ccaacaccac ccggtttcag aaagagctga ttatggataa tctgggatta
     9601 tgccgttttc accgcaactg ggccgaagat ttcatgcctg atattatcgg acaactctat
     9661 ggcgaagaaa tcaaggataa atttaccgaa agtctgaaaa gcattgcata caacatcaac
     9721 agccgtaaca acagtacttt gtgggaatcg tggcgcaatg ttgaattgct tgaaacgtat
     9781 ctcaaacgta agcacgaaga agaaggcaac aatgatccgg atctggttgg ctggattgac
     9841 tatttcaagc gagaccgccg cacagctgcc atcgattact ggtacgaagt gcataaaggc
     9901 atttgtgagt cgatgaacga attctaataa gtggtcagca gcattttctt cagcgatgaa
     9961 tagtgattgg ataacgataa tggttaacgg ttaatggtta ttggttaatg gttaacggtt
    10021 attggttggt tcaatgataa atctcaaaag caacgcgaga agattcaaaa atcaatcgct
    10081 tgattttttt ttgaaatcaa agaaaattca aacacaaata aaaaagccga aaagcagcat
    10141 tcagtcatgt gttgagagat attttacctg aattatatcg cctcaggaat acgatgaagc
    10201 gtttttggct gaacaacggg ctaaaaaaaa tgattctgga gtaggtgaga gacctgaaaa
    10261 taattcatag ttgaatgtgt catttttcag gcagccaata attgttattg aaaaaactga
    10321 cctgcaaaaa tgtctggttt tacctgatga atagtatccg gataaaaaca acacacacac
    10381 aaatggacca gcaaaaacat ccgtaaaaat gattacaaat gtaattacaa ttgtaacata
    10441 ctgttgaatt cttttcatac aaatgtaaat aacacttcta aacatacata gtacttaaat
    10501 acatgataat taataattta aacttactta tttaaagtaa acaatcaaca acaatagtat
    10561 caaatgatat tatccgaaca aaaacgccag aaacaagcaa acagattaat aatcaagcta
    10621 ttaattgata aacactatta attctgttgt tcatgagaat tacaaatgta attacaagtg
    10681 taagcatgta tcaaatacaa atgtaagcaa ataaatgtta cctttgtaat accaaataca
    10741 aacaagcatg atcgaccgca tacaacaact gatgaaaacc tatgagctaa cggctgccga
    10801 tctggcagat acgcttggaa cagaacgctc aggaatatca cattttttat caggccgcaa
    10861 taaaccaagt ctggcattta taaccaaatt gctcgaaaaa tttccggatg ttaaccccga
    10921 ctggttattg ctgggcacag gcacgatgct ccgaagcaat caaaatcagg ttacgcctga
    10981 aatacaagca tatgcagcgc ccaagccaca agaccctgtc aatcaacaaa acaatacaac
    11041 accgcttcca gccaaacaaa taagcgtggc tgaagacgaa gaacccgcag aataccacac
    11101 aaaaaggccg gctgaaaaac caatacaagc cgaaaaaaag caaacaccgt caccagtgca
    11161 gatacctgca ggaacagaaa tagaaagaat agtcatattt tacaatgacc attcattcag
    11221 tgagtataaa atgagataaa agccggtaag acgacttagt cgaagagttc ttcgggcttg
    11281 aacaaaatat tggtttcgcg aatggcatta tcatcactat cggaaccgtg aatagcattc
    11341 atcgtaatat ttgtaccgaa tttaccacga atagtatcgg gcgaagccgc tttgggatcg
    11401 gtagcaccaa caagtttgcg aaaatcatcc acagcattgg cacctgaaat gctggcaaca
    11461 acaattggtc cggaggtcat aaaatcaatc agaccatcaa aaaaatcctg ttttttatgc
    11521 acagcataga attcttctgc ctgttttcgg gttaactgaa tcattttcat tccgcggata
    11581 cggaatccac cggcattgat agtgtggaga atggcgccga tgtgcccgtt ggcaactgct
    11641 ttgggcttaa tgattgcaag tgtaattgat cctgccatag tttaacagtt tagtgaaaca
    11701 attggtgcaa ttttttaact ttgcactttg aaccgtcaaa attaatactt tttgaataca
    11761 ctcgacaaaa atcatatttg caaattcatt tgcactgatc ctcaaaatgt tgtggtagtc
    11821 tcgcatacaa gccctgatgg cgatgccatt ggctctgcgc tggcaatgca aaagacattg
    11881 acggcctttg ggcatcatgc tgtagcgatt cttccggatg tttatcctga ttattacagt
    11941 tggctgccag gggtggagga tattgttata gctgaaaatg aatttgataa agcgcttcaa
    12001 ctcattgcca atgcctcttt gcaggttatt gttgatatga atgccctgaa acgtggacag
    12061 attatcgaaa aagcgctcga acaggccgat gcaaaacgaa ttttgattga ccatcatata
    12121 ctgacaaata atatttttga tgcggcatat tctactcctg atgtgtcttc aacttcggaa
    12181 ttggtgtata atgtgattga ggcgcttaat cctgcattga ttaccaaaga tgtggccgaa
    12241 tgtatttata cgggtattgt taccgatact gccaattttt ttcatggaaa tatgtcgccc
    12301 agaactttcg atattacagc caatctgatg cgcaagaata tcgatattat taaaatcaat
    12361 caggtgctgt atcatacttt ctccgaaaac cgtattcgct tattgggctt tagtatcagc
    12421 gagcgccttc atctgttatc agattttcat gcggcctata tctatctcaa caaagaagat
    12481 ctgattcgct ttaattattc ggagggggat agtgaggata ttgttaatta tggtttggct
    12541 attaaaggta ttcatatcag tgcatttttt atcgaaaaaa ctgactttat caaaatcagt
    12601 cttcgttcgg agggcgatat caatgtgaat gcaatggcat cgaagtattt caatggtggg
    12661 ggacataaga atgcttctgg tgcacatttc tatgggtctt tgacagaggc aatatcgttg
    12721 tatgaacaag cgttaaggga attatgatta aatcgttgtt ttattttttg tgttgttttc
    12781 ttttgctggc ttcctgtggc gagaaatcaa atgataatac gggaatgctt gatcctgagc
    12841 gctatagcaa aaatcttgaa aaagcaaata acctatatac tcagagtgaa gatggtcaga
    12901 tcgatgattt tgtttcgcgc aatcgctggg agatgaccaa aaccggtagt ggcctgcgtt
    12961 atatgattta caagacaggg cagggtcgga aagtggctga taaaacggtt gttcgttatc
    13021 atttcagggt taatctgatt aatggttctt tgtgtgatga atcagccaaa accggcccaa
    13081 aggagatatg gattggtcat gccgatgtgg tgagtggtct cgaagagggg cttttgttgc
    13141 tgcgtgaggg tgataaggcc aagtttatta ttccgtcgca tttggcttat ggttggattg
    13201 gtgattcaaa aacaattcct tcgcgtgctg tcctggttta cgatgtcgaa attctccagg
    13261 tgcgtgatta taatgttgaa taatatgaga aatctttttc tttgtttctt ggtcgctctg
    13321 acggcctttt cgtgttcgca atccgattat cctggctacg atttatctga aaatggtttg
    13381 aattacagaa ttgtggatga tcaggacggc cttcaggtgg ctgtgggcga ttttatcaat
    13441 gttagaatcc agtataaaac aatgagtgac agtgtgtttt ttgatagtga tcaggaaatt
    13501 gtccctgtct ggattcctgt taataaacca tcttttcgtg gtgatattat ggaaggtttg
    13561 gcgatgttgt cggtgggcga tagtgcttcg tttgttgtgc gtgccgacac ttttttccga
    13621 atgaccatcg gtgcgttatc attcccttct tttcttgaaa aggattcaat gatgtatgtc
    13681 aatctcaggg tccttgaagt taagactcaa agcgaatttg ctcaggaaaa acaaattctg
    13741 gctcaggagg tcgaaaagca gtttgccgaa ttgaaagaaa aagaaatgct tgatctgaaa
    13801 tattatctga aaatcaataa tattactcaa acaccaacgg agtcggggtt gatttttatt
    13861 ccggtcaaag tgggttctgg tccgcgtttg attaccggac aaacggtcag atgtcattat
    13921 accggaacat tgattgatgg acagattttc gattcgtcgg ttggaaaaaa tccactggaa
    13981 gttgtggtgg gttcgcctga tctgattgat ggttttaatg aggcattgac aatgatgtcg
    14041 gtgggtagtc aggcaacggt cataatgcca agtaaaattg gttttgggcg ttcagaaatt
    14101 gattcgccta ttccgcctta tgctacggtg atttttgata tcacggtggt gagtgctgag
    14161 tgagataaac ttctcgtagg attttataac agggccaatg cattaaaaca tgcatgaaac
    14221 ttcctgaatt tcagttacac aacgttgcac aaagtcctgc acaacgtcgc acatagtaaa
    14281 aagtgagcaa aagccgtttt aaaaagcaca gcgcagttgt gttaatcaat tatagcatat
    14341 cgaattctga aagctcgctt tcaaattcac tttgctgtaa ttgatatcgg cttttgtctg
    14401 attaaatcta ctctgtgcga ctcagtgtgt actcctttat actttgtgtt acaaaggttg
    14461 atattgcatg cttgaagtca ccatcgggtg catcaatcag aagtattcgt tagcatttaa
    14521 atgcgttgac cctggatttt aaaccctgtg aagggttgtt tgaataaaat aaattttata
    14581 tttgcagaaa acaaaccacc atgaagaaaa aattatttta tgcactgttt ttaggcgccc
    14641 tgactgttac gggtttgggt ttcagttctt gcagcaacag caatcctgat tcacaaaaca
    14701 aaaccgaaga tgtaaacgat gttgatgttg aagaagaaga tcttttcgtc agcaaagatg
    14761 gaaagtttaa ggccaatttt ccgggatcac ctacccgcga tgttcagatg gttgctacag
    14821 aagcaggcga catcgaaatg atttcgtttg tgtatgaaaa aagcgctact gaagccagca
    14881 tgattgccta cagtgattat ccaagttcgt tgattgatgc acaggatccc atgtccattc
    14941 tcaaaaatgc acgtgacgga tcgatggaag gtaatatcgt tgaatcaaac gacgaaatga
    15001 aagtaaatgg ttggcctgct atcaggacca gagccattga caagacacaa aatctgcatt
    15061 atgttcttga ggttgttctg gcaaaaaacc gtctttatca gatattgatg gtgcgcgatg
    15121 gcagttatcc cgatgatgct gctgttgaag aattttttga cacttttgag attaccatga
    15181 agaagtaaat gcattatatt cttcaaaaaa aaaaccgcaa aaacatttgc ggtttttttt
    15241 gtgcttttat tcgccaccga ttctgaatcc ttgtttgtag ccggtaacat aaacctcgaa
    15301 ataaggatta cccgacgaca acggcgttag gtcatacacc agatggtgtt ttacctgttt
    15361 tttaccttct gttgcagcca ctgagggcaa aatattcaga tatgccttgg gaggatacga
    15421 tttaagccat gcgccattaa aagccatggc aaatgaaacg tcggtttttt cacctgtgta
    15481 cgttagtata gtgtgcaggg tcagaccatc aacccaaaca gtatcaatgg tataagaaag
    15541 cttttcggtg gccatttttt ccccgaattc agttttgata atctgaattt gttttgtatc
    15601 ctgttgtttt tcaacaattt tgttgcattt acacgaaaac aagcccgttg aaatggccaa
    15661 taacattata atctttgagt agtttttcat agctgttttt tttatatgga taacaaaatt
    15721 aatacctttt cgttaaaaca gccgaaataa catttattac ctttgtctca aataacagat
    15781 ttgaaaccac atattgtctt tcttgcggcc tggtatcccg atgagcgcga tccgatgttt
    15841 ggattgtttg ttcataaaca tgcggtactg atgaccaaag aattcagcgt ttcggtcttg
    15901 catgtaacca attacagcga caaagccaac ggaatggttg tttaccataa agatggagtg
    15961 agtgttcatc aattgaatat taaaacaaaa aatcgttttt tgcgttggtt atatttcttt
    16021 gttgccgggt tgcaggccta tcgttacata tgtaaaaaag aaggacaagc ggtattgaat
    16081 catgtacatg tattaacccg tatgggtgtt ctggctgtgt ggatcagatt ccgcaggcga
    16141 ataccctatg tgataaccga acattggtcg cgctattttc caaaccccgg aacataccga
    16201 aacgttattc gtaaatgggt taccagattt gtttgtcgca aagccagcgc aatgtcggct
    16261 gttagctgcg gactgaaaga tgcgatgata aaacatcgtc ttggtgttga taaacaatgg
    16321 tttattgtca ataatgcagt taatgaagat gtttttgatt tacccaccga caagcccaac
    16381 aatgttccta taaggtttat caatgtaagc tgtttcgaag atcgttcaaa aaacctgagc
    16441 gggcttgtca aatgtgtcga aatgttggtg atcgagggac ttaaaataga atgtgttttg
    16501 gtgggcagtg gcgaagattt taatgaaatc agttctttaa tcaagtcgaa aaagcttgaa
    16561 aaacagttta ttatgacagg tcaactcaat gaaatgatgg ttgcaggtca gatgaaaaaa
    16621 tgccattttt acgttcagtc gagccactat gagaatgttc ctgttgtgat ttctgaagca
    16681 cttatgtgtg gattgcctgt tgttgctact aatgtaggtg gattgaatga aatgatagat
    16741 gatagcaacg gatatttagt tgaacacaat agtttaacag aattgtcatc cgctatccga
    16801 atgatgacag aaaattattc agcatacgac cggcaaaaaa taaggcaggc agctatgcat
    16861 aaatattcgg ctgttgcagt cagacaacaa atgttacaat tgtattcagc gctaattaaa
    16921 tgattgacaa acaagaatat ataagctatg ccatcatggt caacggtcag catctcaaac
    16981 gttggcaggc cgaagccata gaaatactca atcaatcggg attatgtcgc tgtgtgctta
    17041 tcattagcaa tccagatgat accagtcata aaaaaaatgt gtttggcaaa ttattgaatc
    17101 gtcgattatt gtataacttt ctgttgaata gatatttcaa tgtcggggct gaacaaatgt
    17161 gcactttgcc gaccgatgta cccattatag aaatagcggc agagcaaaaa ggcgtatcgc
    17221 actattttac cgaagctgat atattgaaaa tcaaagagtt tcggcctgct tttattgtaa
    17281 ggtttgggta taatattctt cggggtgcca tacttgaggc agctccttat ggcatttggt
    17341 cgtatcatca tggtgatgag cgaaaattca ggggcggacc ttttgggttt cacgaaatca
    17401 gaacaaaaac accggttagc ggcgttattc tgcaaaagct tacttcgcgc ctcgatgctg
    17461 gtcaggtttt gcttcggcgc gaatacataa ccgttatgca cagttggaaa gaaatgcgac
    17521 aacgtttgtt ggcggccaac agcgatatgg ttttgcaggc agtcaaaatg tatattatta
    17581 ataatgagaa aattccagcg gcctcgacaa cgcaggcacc tgtttttaaa gccccgggca
    17641 atatgcgtat gctgtggttt attgctgccc tttggatgcg aagggtttca tttcatatca
    17701 aacgtttatt ggtttacgaa agttggcatg ttgaaagtgg gctgacctac aattcgccat
    17761 tggtgccggt tttgcctact gttacaaatg tatcagcaag taattccgga gcgtttcatg
    17821 ctgatccgtt tttattgcaa acatctgata atctgtatgt tttgtctgaa ttcttttcgt
    17881 acagtcgaaa attgggttct attactgcca atcgtgatga acaggatggt cggctttggc
    17941 tgcaaaaaga tacgcatctt gcttatcctt acgtgtttag ttattcaggg gagcattgga
    18001 taatccctga aaatgctgat tcgggcaaat gcattgctta tcgtgttgat aatgagctta
    18061 atattataga agagcttgtg ttgcttgatt tgccggctgt cgatccttct ttggtgtttc
    18121 atgaagaaaa attttatctt ttttgtggac ttaaagatca gttacccaat gaaaaactat
    18181 ttgttttctg gtccgatagt ttaggtgggc cttaccatgc tcatgcacag aatcctgtga
    18241 aagtgagccc agttggatcg cgtccgggcg gaagcatgat aaattggcag ggaaatttgt
    18301 atcgtcctgc gcaggtttct gatatttatt atggatataa aatattaatt tacaagatat
    18361 taaaattatc accacaatta tttgttgaga tagaaaagag cgagatcaat cctgccattt
    18421 ttggcagtca atataatggt ttacacacgt actctattta tggtaatcat tatgcggttg
    18481 atctgaagac gcatcgggta ggactggctg cattcatttt caaatggaaa caaaacagaa
    18541 aaaaccggag ggccgatgct tagtaatctg cttcgtacgg ttggaatcag gattgcaacg
    18601 gctgttatta gtttgctgat tgtggttgtg tcaggacaaa tgcttggtgc tgaggtactt
    18661 ggtgaaatca gtttgtttat tgtggcggtt accattatta atttggtggg tggttttgca
    18721 ggtggagctg ctttggtata tcttattccg cgcaacagac ttttgggctt gtttgtgatt
    18781 tctcagctat gggctggagt ttcgggtttg tttacaacgt gttttctttt tctaattggt
    18841 gcatttccgc aaaatcttgt ctggcatgtt atgttgcttg gtattgtagg ttcgctgaat
    18901 cagaataatt tatcggtttt acttgccaat cagcgtataa aagctcataa tttacttaca
    18961 ttgcttcaat cggtgctgat gattggattt ttgatgggtt ttacttttct gtataagatt
    19021 gatactattc ttgcctatat tttatcaatg tatgcttcgt atttgccggt gctgtttctt
    19081 tcgtggatgc tggccggaaa aggcactcaa acagggaagg tgagtccgtc gcaggcctta
    19141 aaggtgcttt ttggttatgg aagtctggtt caggtgagtt ccattcttgc attgcttaca
    19201 taccgcctta catattatta taccgaggcc tggctgggat tcgcagctct tggacttttg
    19261 tcggttgctg cgcagatcag cgaagctgta tggatcctgc ccaaaagcat tgcattggtt
    19321 cagttcagca aaatctcgaa tttgaaaaat gatgctgact ctgctcatct gactttattt
    19381 cttggtgtta ttacttctgt ttttaccttt ttggctttgt cggttctggt gtttttaccc
    19441 gatcaggtct atactttcat ctttggaaaa gatttttatg gggttggtgc ggtggtgcgg
    19501 atttttgcac ctggcatttt tgctatgtcg ctcaatatta ttctgagtca tttttttagt
    19561 ggtactggca aaataatatt caatctggtc ggatctgcta ttggtttgct cactgttgct
    19621 atttgtggtt caatttttat taaaaatggc aatataagcc atgctgccat ggtttcaaca
    19681 tttggttatg ctgtcaactt tatatttgca tggctttgtt tctggtggat tacgcgccgc
    19741 aatagtttat ccttccgaaa gggactgctt gtttttcgcg gggtgaaaaa gtgaagcatc
    19801 ttcgaaaagg tctatggaat tgacctgcgt tctcaaaaag caatcaatta gttatttttt
    19861 cctgtaagaa tcccaaccga ttttttatta atggtaaaca tgtcaaacgt aacataatcg
    19921 ataatgagat tttcaccttt cacattttcg agcaacagca caaccggata aaatgagccc
    19981 cttaatggtc ttgtaaaaga aatgtcagcg ctgaagtcca tcactttttc accttttttc
    20041 aagccgttac ttacttttac ttctccgcac ggaattcctt tcagactgcc gccttcatat
    20101 ttgttttcac ataaaaacag ttgaatagat aacttgccgg ttgtttttga gtgaatgttg
    20161 atgattttgt cggcggctac aatggcggag ttcgagtcaa ggtttatctg atagcttgtc
    20221 agaccttcaa agcgcgcgct ctgggaccag gaaatcagag gcagacatac gagaagaata
    20281 atgaagaggt aaggaaattg tttcatgggg ggatttttta attatttgtt ttgaattact
    20341 tgtcaatatt aatgagccgg tatttgatgt tacccatact cattttggca gcataggcaa
    20401 tctgatgctt tccgctcacg gaattttctt tcagaaaagc gtcgtcgcat ttgtgggctt
    20461 tgtcaaccag atcgtgtgca gccgcttcga tggctacgat atcgaacgat gcaagtatgc
    20521 cgatatcggc aacaaatggt tttttgggat tccgcgcgca atcgcagctt ggtgatacat
    20581 tgatgattac gttgaaatag attgaagttt tcaattcaca cacaggcttg gcatattcaa
    20641 caaggccttc gagaaaacgt cgggttttgt ctgtatcagt tggatttgaa atagctgcaa
    20701 catggcatga agtgatgcat tttccgcaac ctatgcactt tgccttgtcg atatttagcg
    20761 gattgagcgt tattacaccc accggacaat cggccacaca tgcgccgcat ccgatgcact
    20821 ttgtgctgtc ggttttagga aagttgttga agtgcatgag ttttttgcct tcgatggtag
    20881 ccattcccat cgacacattt ttgatgcatc caccgaaacc cgcactgcca tggcctttga
    20941 aatgggtgaa ataaatgatg gtttcgtatt tttcaatgtt tttacctatc cgggccgagt
    21001 cgaaccaggt gccgccttcg attggcaaca taagcgttcc agccgagtcg aggatatcga
    21061 tgggtgcaaa agtgaaacca tgttctttgg ccagttcaat gtgggtgccc gtttcgtgcc
    21121 gacgtccctt gtaggcaacg ttagtttcga taagagtggc attgagttcc ttgcaaagtg
    21181 gttcaaccag cgtggccggt agataatagg cgtttccgtc ttcgccaaaa tggactttta
    21241 ttcccacatt gccatgcaca ctgtctttca tgaatgtaaa cagtttcatg actccttccg
    21301 gagaaatgtc ttcggtaaaa taaacatcgg atcgtttatc gttgttctgg caaaatgtat
    21361 tctggctgga aatgagtatc agcacaaaga tcagggaagc gagcagtctt gtcataaaag
    21421 tattttgttg taataatcag gtacaaaaat actgtaaatg caacaaaaaa gtaagtgtat
    21481 tggcgcagag tgaataaaaa tgtgtgtatt tttgtctgcc agattcagaa atagcccatt
    21541 ggtttttaaa accactttct gaaacccgca tctgcggcag tagctcagag gtag
//
LOCUS       DAOPKE010000049        21579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2588_length_21579_cov_12.961113, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000049 DAOPKE010000000
VERSION     DAOPKE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_21579_cov_12.961113"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            582..1325
                     /locus_tag="PKY63_07630"
     CDS             582..1325
                     /locus_tag="PKY63_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00520.1"
                     /translation="MKYSFLILIGSLILFSCSGGENSNEDENKSFSTTENVKPKKSPL
                     QKSIDKYKKEAKENLTPLLQKFTLVEDKVEGGGWYYHKNRKSKYQSHIEVPISTNGYF
                     YLKTNYYGSDWLFHNQIIVNIDGTSYYSSIIENHDDYSVTDVLGGGVLEILHLTDPQL
                     DAHIVSLIFTNQDEEIIVRFKGDQAEKDITLSKADKEVIKDCFLLSQYLTVTKNKKVS
                     KIEDNALKDGFYLIPNGNVPEASIKLKSE"
     gene            complement(1567..1923)
                     /locus_tag="PKY63_07635"
     CDS             complement(1567..1923)
                     /locus_tag="PKY63_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00521.1"
                     /translation="MKNKELVVVRGINSEWHGENFSNLRISFGELPQTIPSEDAYYVG
                     LYLEAPESKITHIGIVKSIDRYNNGADFNLKCIIKLSKPIDPGHPIRKHENWTLTDLG
                     LNPKEMEELRIKLMQI"
     gene            complement(2075..5527)
                     /locus_tag="PKY63_07640"
     CDS             complement(2075..5527)
                     /locus_tag="PKY63_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00522.1"
                     /translation="MRRIIGLIVLLTAFVQLTAQEKPGMKSLSLQLPVPDSIQLDSFT
                     VDPADIKIILPDGTIADTSYFRFDISKGLLYFQKAKFPATGKLKIEWKAMSVNLSKPY
                     KHKDTTLLFPELNNYYNPFDATASGSSGSWLETDGLDKSGSITRGFSVGNRQDFSLSS
                     SLNLMLNGKLSNDLEIQAAISDNNIPIQPEGNTQQIQEFDKIFIRLSKEKNIFTAGDI
                     EVQAAPQSYFLKYSRKAQGADFQTTFSPGLTKNDELFVRANGSFGKGRYTRQEINAIE
                     SNLGPYKLKGNNNETFIIVLAGSEKIYIDGNQLTRGEDNDYIIDYNTAELTFTTKVTI
                     TSKSRIVAEFEYSDKNYSRSLITSEIGYKSGKFTTGLSLYKESDLKNQTIQQDLSDEQ
                     KMIMSLVGDSIQNAYSSGVDSIGFTTERVMYAMIDTLGYDSVFVYSTNADSALYALTF
                     SYVGKNNGNYVQTGSSANGRVFKWVMPVAGVPQGEYEPVMMLITPKKTAMYAFRAGYD
                     FSKNTALHAEFAVSNKDFNLFSSINDADNSGHALTIHFRNLQKIGNDTIKKWSLTTNI
                     RTEITDRTFNAIDRFRSLEFDRDWNLDLMENRGNTYFGLADVVLQNTKADKFRLSSGF
                     FMRNPENASYQNSLQINKNWKSTSFRSAAVYLSSPTHFNKTDFVQHQNKLEQKLGNFA
                     LGAEANGESKLIHNAATDSLHSGSFDWWLAKMYTGYNDSMRSAKLFYQYREDNISDSN
                     RLVPQSYMTDIGFESSGQLAENNQLLLRATYREVIYKTGNVSNDEFLLGRLEYSGRLK
                     KRFIVLGAAYEAGSGMEAKKEFSYLEVAPGQGVYAWNDYNGNNIRELNEFEPAVFSDE
                     ANFIRVYTPTNDYIRVFSSQLSGNVAISPADILRKKKFTGKLISKFSNRSAFSFDKKT
                     SSDLASEMYLPFTSEGPDSLLVTQNATWRNVLTFKTNDSRFAINWINSANRARGLFTN
                     GIEDRQTRQDEIKISKSFLKSWITELSAISGLRDFTSEYFTSNNCSIEETGIKPSLSW
                     QPGTTFRWTMLFQYSEKNNVLSVEKAIVRKAGTELKFSKSGKGLAMLQFNMLQISFNG
                     DLNTNLGYIMLDGLQPGRNFTWNLSIQRTLGKNIQLNFIYDGRASEDNPVIHTGNVQV
                     RVFF"
     gene            5804..5877
                     /locus_tag="PKY63_07645"
     tRNA            5804..5877
                     /locus_tag="PKY63_07645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5838..5840,aa:Ala,seq:ggc)
     gene            5910..6935
                     /gene="rlmN"
                     /locus_tag="PKY63_07650"
     CDS             5910..6935
                     /gene="rlmN"
                     /locus_tag="PKY63_07650"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPF00523.1"
                     /translation="MEKNTLFGLLPEEIADIIEKYGARSFTAKQLAEWMYAKRVFSYD
                     DMSNLPKQLRENLKTDFPVILHPYSNVQVSKDGTKKYLFEFEGGRVVETAVIPDDDRL
                     TVCVSSQAGCRYGCRFCATGAIGFLGSLTAGEILNQLMAIDEYDKITNIVYMGMGEPL
                     DNWEEVYKSLEILTSEKLMNMGAGRITVSTIGIHEHFNELIEKTRVNIAISLHSPFPE
                     ERAHIMPAQNSNPVGEILDTIAHADIPRHRKLSFEYILFKGINMSKSHASKLADLAMR
                     CHAHINLIAYNAVPGLPFEAPAQKDVLAFQDELLKRGASATLRRSRGLDIAAACGMLA
                     GNKLEES"
     gene            7229..7612
                     /locus_tag="PKY63_07655"
     CDS             7229..7612
                     /locus_tag="PKY63_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00524.1"
                     /translation="MSTSILDQYLNNLKFIVLTDRETGQAIEIYFDYTLPNMHSEIYG
                     QAVSEYAAKGRRIGISGGGRITKIDNYIVLHSRSGNYGRFEEEDALRLAPLHPMLCDQ
                     GFVFLSMSGEDNAMVVVREYEATLK"
     gene            complement(7662..8321)
                     /locus_tag="PKY63_07660"
     CDS             complement(7662..8321)
                     /locus_tag="PKY63_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00525.1"
                     /translation="MCFSATASFAAGAVLTAIGAVALKKTTKPQQLPFAGIPLLFAAQ
                     QFAEGFLWLSLSNSEYAIFKEPCTYLFLFFAQIFWPTWVPFAVLKLETSEKKKKILKI
                     MLATGILVSLYFLSCMMIFPVDGVIQECHIFYTFGYPVIMTPIVSVFYAMATIGSLMF
                     SSVKGMKLFGVSVFIAYLVTGIFYLDYFVSVWCFFSAILSLIIVSVIYRLRLSVTEPI
                     L"
     gene            complement(8437..8781)
                     /locus_tag="PKY63_07665"
     CDS             complement(8437..8781)
                     /locus_tag="PKY63_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00526.1"
                     /translation="MKAKFFFIIIAFSFLYSCENNYDHNWDERIRIPNSFSPDNDAYN
                     EMWCIDADDVAKCLLIVTDQDGIEMWRTTNIYTCWNPGSSISGGLYYYYLHAEFTDGL
                     QHDYSGELYLLH"
     gene            complement(8786..9667)
                     /locus_tag="PKY63_07670"
     CDS             complement(8786..9667)
                     /locus_tag="PKY63_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00527.1"
                     /translation="MRKYICLFLLIPFLSTAQDSLRVPVLFPGEACSVFPQNPGEAFF
                     KASAGPGFSVDKPSVYINSDAEICFNPSKKSRFTYTADWNYTASYMYKTSNGGISVLY
                     DFLKNPDYSLAAGMSFQVLARKLNWESLVFPDQLTIPDTIIESNQVKPIDQLSNAVAT
                     FHGHLVFTAPHMFATARFYDLTTPNISFVSYNEYTGGMRVTAGYHNDSRNGFRTIATG
                     WRMNQLQHHIFNIESTYQFHRVCLGIQLSTNKSGGVMLMYSGKKISVGVASFAFIRPV
                     SDEFSLQQSRLVFSINL"
     gene            10029..11033
                     /locus_tag="PKY63_07675"
     CDS             10029..11033
                     /locus_tag="PKY63_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPF00528.1"
                     /translation="MKISASLYSANATDLRELVRELDAYQSDWFHIDCNDDSSVFEDI
                     REIRKYSDTPIDLHIISPEPHKFYDVIVETGVEYVTLQFETIENWHPMPDSYKGQLGI
                     AITTDTDISVFDRFANQASFILFMATTPGKSGEPFQQKNFRKIREFRNKYPDKKIHVD
                     GGINAELSFILRNMGVSAVVVGSYLLRQEFIGSAMMKLRSNTGSEYYVSDFMLKRDEI
                     PAVTKNNYSFPQLLKVIDDYKLGFANIVDENDTLLGIASMADVRKALLKYWPNLDDLK
                     VEDVMNPHPALIQSSNTVFDMLDYVNNLSFPVLFLPVTDDNGKLVGTVKFNNLIKGEL
                     "
     gene            11030..12022
                     /gene="aroF"
                     /locus_tag="PKY63_07680"
     CDS             11030..12022
                     /gene="aroF"
                     /locus_tag="PKY63_07680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPF00529.1"
                     /translation="MIIQLRKNISNPQIDTLAAAYEGVVVNYNSNVLVVTPSAIKNID
                     SKFDDIVEKNWVLDSDIQLSSRKFKTETRKISIGRYTSGSAQTLLTIGPCGVEGEEMM
                     EQTAKLIVESGLTSIRAGAFKPRTSPYSFQGMGEDGLKLLAKMREKYGLSVVTEVRDS
                     THVQLVLDYADVIQIGAKAMYDHGILRACGRSQKPILIKRGFGTTLQEFVQAAEFVLS
                     GGNPNVILCERGIRTFETKSRFTLDLCGVAYLKENTNLPVILDPSHAMGYAYGVPDLA
                     RACMAMGVDGLLIEVHPNPAVAKSDASQQLNHETFRNLFESLKPIAAAVGRTIV"
     assembly_gap    12291..12390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12391..12954
                     /locus_tag="PKY63_07685"
     CDS             <12391..12954
                     /locus_tag="PKY63_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPF00530.1"
                     /translation="NWPKPSELAQMADYMSLPFESLLCPAAEIPADFECKLLAIDIDG
                     VMTDGGMYVTSDQKEMKKFNTRDGMAMRIAARLGIEVGFISNGQMEGLIGYRAEMIHV
                     KRWYVGNEKKLPVLENWCLELGISLSQVAFIGDDINDLEVMQQVGLSACPADAVPAIK
                     AVSKIVLQTKGGHGCVREFGEMLGWTR"
     gene            13134..14081
                     /locus_tag="PKY63_07690"
     CDS             13134..14081
                     /locus_tag="PKY63_07690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPF00531.1"
                     /translation="MDSKVALFSGQATRPLAEKIAESYGVKLGNVELMRFSDGEFQVS
                     FEQTLRGSELFLIQSTMAPSENLFELLLMVDAAKRASAHHINAVIPYFGFARQDRKDK
                     PRVAIGAKMVANLLMAAGVDRVITIDLHADQIQGFFDIPVDHLFASSIFIPYLQSLNL
                     GNNIVMASPDTGGTKRANAYSKYLDCDMAICYKQRSKPNQISNMTLIGDVDGRDVVLL
                     DDIVDTAGTLCKSAELMMNAGAKSVRAMCTHGVFSGKAYENIDNSVLTELIVTDTITK
                     DHKSSKVKVLSVAELLADVIKRNLTCESISTHFNLNTLI"
     gene            14092..14703
                     /locus_tag="PKY63_07695"
     CDS             14092..14703
                     /locus_tag="PKY63_07695"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPF00532.1"
                     /translation="MRTVSMSGSLRGNVGKKDAKRLRNEGLVPCVLYGQKEQLHFFTQ
                     EKQFKDVIYTPNACFVELNIDGNKHMAILQEVQYHPVSDSIMHVDFFEFANDKPVSLT
                     IPISLTGTSPGVLKGGKLQQKVRKVKITALPEKMPEKLIIDISPLDINDAVRVKDVVA
                     ADYKINLPENNVLIIVTQTRNVVDETAATEDAAVPAAAAAPKK"
     gene            complement(14804..16573)
                     /gene="sppA"
                     /locus_tag="PKY63_07700"
     CDS             complement(14804..16573)
                     /gene="sppA"
                     /locus_tag="PKY63_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPF00533.1"
                     /translation="MKEFFKMMFASMTGCLITVLVVFVIMFAVISGLMSGGSEEVKIQ
                     DKSVLVIELNKAITERTSDNPFDNMDFGSMSSETAMGLNNILRTIKYAATDERVSGIL
                     LDLSDVNAGFATTEAIRNQLDTFKASGKFVIAYGDDFSQKAYYLATAADKIYLNPEGA
                     VDFKGIYMQTMFFKNALAKLNVDVQIIRHGKFKSAVEPFLLEKMSDENRLQLQTFADG
                     IWGHLVKEIAASRKSDTAKMNMVADSLFALNAEGALKYNIVDSLIYRDELNKLILTKS
                     VQTGDIPYISMNDYYKGIKEKLLSYNKDRIAVIYAFGEINNGKYQHGTISGKHMSKVI
                     REAAADSSIKAIVLRVNSPGGDALASDMIWREVYMANKIKPVVVSMGDYAASGGYYIS
                     CAARKIFAEPTTLTGSIGVFGMIPNMQKLFNEKLGITFDEVKTNQHSDYIGTMKPLNE
                     YDRATLQFMIEDIYSTFIKHVAEGRKMKPEDVDSIGQGRIWCGTDAMRLGLVDEMGGI
                     YDAIAYAAQAANLTEYSVEELPVQKDFLATLMEDFGSEAKVESGKIIEQELGEFAPLY
                     EAWKKATQMSGVQARLPYFLLMD"
     gene            17039..18115
                     /gene="fbaA"
                     /locus_tag="PKY63_07705"
     CDS             17039..18115
                     /gene="fbaA"
                     /locus_tag="PKY63_07705"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPF00534.1"
                     /translation="MEKLSSLLEPGFVFGEKMRVLLNHAKANNFAIPAVNVVGTDSIN
                     AVMEAAMQAKAPVMIQFSNGGGQFYAGKALPNDAYQASIAGSVSGAEHIHRVAAMYGV
                     PVIIHTDHAAKKLLPWIDGLLDAGEKFYAQHKKPLFSSHMLDLSEESLEENIEICKKY
                     LARMSKIGMTLEIELGVTGGEEDGVDNTNVDNSKLYTQPEHVAYAYEELMKISPDFTI
                     AASFGNVHGVYKPGNVKLMPVILKNSQDYIQNKFNTQEKPVNFVFHGGSGSAREEIRE
                     AISYGVIKMNIDTDTQWAMWEGVMNFYKKNEGYLQGQIGNPEGEDKPNKKFYDPRVWL
                     REGEKSMVKRLLVAFEDLNAVGTY"
     gene            18298..18978
                     /locus_tag="PKY63_07710"
     CDS             18298..18978
                     /locus_tag="PKY63_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00535.1"
                     /translation="MRRYMSGKYKTILVILFMACKGFAFAQTVLFHEYEMTASSWSLL
                     RWNVDDTTGVKNILRETVDAQGRVSSLEFLKNGKIFTDKICYLPEKVEYEYTDSTITE
                     LLYSDGALMYQNDCEMWFKTVYHLDSNNFVFEKEVFSRKDTSEMSPDKTRWLDENLPE
                     YQLEELSPESYFFVTYYYYSWAKLNGLMPVSVTYDPSAFDVYDSEIPAWNKVIEGIEK
                     LRQQAEMK"
     assembly_gap    19239..19338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19339..19593
                     /locus_tag="PKY63_07715"
     CDS             <19339..19593
                     /locus_tag="PKY63_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPF00536.1"
                     /translation="PMCIPVYAVMEDLENEYSHVKFADMLFDSPEAHVIRNAPEARGF
                     MGLPFVMYYKNGKVVKAVSSIQSEEQVREALEESFGVVVK"
     gene            19599..20537
                     /locus_tag="PKY63_07720"
     CDS             19599..20537
                     /locus_tag="PKY63_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF00537.1"
                     /translation="MNEQNKFDVIVIGGGPAGLTAGIYLSRAKLNTLILNVGVAGGQM
                     ILTHEIANYPGVESISGYKLASTMKKQAQSFGATIKTISAPPEVVDEGIFKIVRAGEN
                     TYSAHSVILAPGGAPRMLGVKGEQEYKGVGVSYCATCDGEFFTGKNIVVVGGGNSALE
                     EAVSLSRYAETVTIVHQFDHFQAFPAYVEEAKNNPKIKFIMETEIREFAGDGKVERVL
                     LHNMKDEKEEWFETEGAFIFIGYQPNTTFVKDLLPLNERGEIVVNADMETAVSGIFAA
                     GDSIAKKYRQVTTAVGEATVAALAAAARTWEIKSNH"
     gene            20549..20971
                     /gene="trxA"
                     /locus_tag="PKY63_07725"
     CDS             20549..20971
                     /gene="trxA"
                     /locus_tag="PKY63_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF00538.1"
                     /translation="MPVWLIVLISVVGLFILLIVRSYLKLKNASNDAGSEHLLHLTDV
                     DFQKKISNGVTLVDFWAGWCMPCKLLGPTISQLADDNAGEVKIAKLNVEESQRTAAKY
                     GVSSIPTVIIFKNGKEINRIVGVKPKQHYQNLIDKALK"
     gene            complement(21191..>21579)
                     /locus_tag="PKY63_07730"
     CDS             complement(21191..>21579)
                     /locus_tag="PKY63_07730"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-asparaginase 1"
                     /protein_id="HPF00539.1"
                     /translation="PRVGILKLFPGMPDAYTRPVLLQEELDVIILETFGSGNAPTTSS
                     FVKNIHEAVSSGKVLFNVTQCKGGMVEMGKYAASLPLMNLGVVSGNDITTEAAIAKAM
                     FLLGQGYKDKELATMLQKPLRGEMNQ"
BASE COUNT         6043 a         4288 c         4845 g         6203 t
ORIGIN      
        1 aaagcttaaa cttcgccttt tttgagcttt attttgagtt tggatgagct ttaaatagtg
       61 gctgtctcaa aaacattcca ccgttccggc caaaatcaac cctcgaagag ttccgaactt
      121 ttcgagggtt ttctaaacag gccggaacat ggaaccgtcg taacaatgag caaaaacaac
      181 ggttccatga catccagctc cttcgtcgct ttcggacgat ggaatgttgt ttttgccctg
      241 tcgattgtta ttttgacagc ctctaagtag ctatacaaac acctttaatt gcacaaattt
      301 tccactcaca aaacaatttt tcatatattt gcttctgcat gtcgtaaaaa acatgcgttt
      361 tattgaaaag aaagcgtaag ctattgttct tgctgtcaaa aaccgccagt tgaaagcaca
      421 aacaaggata aaaagcagac gctcacgcta cggcgtgggc tgtgcttatt tgtttgtgca
      481 ggtgcctggc ggtaccttgg cagcggatat agtttcagtc ccacgctttt ctattttatt
      541 aattgtcgct tggggattgg gtgaaaaaaa tcttacagac tatgaaatat tcatttttga
      601 ttctgattgg ctctttaatt ctcttttcct gctcgggtgg cgaaaattcc aatgaagatg
      661 agaacaaaag cttttcaacc acagaaaatg taaaaccgaa aaaaagtcct ctacaaaaat
      721 caatagataa atacaaaaag gaggcaaagg agaatttgac accactttta caaaaattca
      781 ccctcgttga ggacaaagta gaaggcgggg gttggtatta tcacaaaaac aggaagagca
      841 aatatcaatc gcatattgaa gtgccaattt caactaacgg atacttctat cttaaaacaa
      901 attattatgg atctgattgg ctatttcata atcaaataat tgtgaatatt gatgggactt
      961 cctattattc aagtatcata gaaaatcatg atgattacag tgtaactgat gtacttggtg
     1021 gtggtgtgtt ggaaatattg catcttacag atccacaact tgatgcacat atagtgtctt
     1081 tgattttcac aaatcaggat gaggaaataa ttgtaagatt taaaggtgat caggctgaaa
     1141 aagacattac tttatctaaa gctgataaag aagttataaa agactgtttt ttactttcac
     1201 agtatttaac ggttacaaaa aataaaaaag ttagcaaaat tgaagacaat gccctaaaag
     1261 atggatttta tcttattcct aatggaaatg ttccggaagc atcaatcaaa ttaaaaagcg
     1321 agtagaataa ctatttagtg cagcaatgtt aaaaatgaaa tggaggccaa aaacaattat
     1381 gcttatggga cgtatttttt aacatgaaaa gcataaatat tttaactgtg ccttggattt
     1441 tcttcgagta ggcttacctg gtagtcaaaa ctaagaggtc ggcctattca agaaattacg
     1501 cctgcaacct gtgagaaaat ttctccaact atattaccta atccaaagaa atggtatgac
     1561 aaaacactaa atttgcatca gtttgattct taattcttcc atttctttcg gatttaatcc
     1621 aaggtctgtc agagtccagt tttcatgttt cctaatcgga tgacctgggt caatgggttt
     1681 tgacaacttg attatacatt ttaaattaaa atcagcgcca ttattatatc tatcaatact
     1741 cttgacaatg ccaatatgag ttattttaga ttccggcgct tctaaatata accccacgta
     1801 atacgcatcc tctgatggta ttgtttgagg caattctcca aaactaatac gaagattgct
     1861 aaaattttcc ccatgccatt ctgagttgat cccgcgaaca acaactaatt ctttgttttt
     1921 cacattcatt ttgtttttaa tttaatgttt aagttacact taatttcaag aaactacaat
     1981 aagtttcata catacaaatt tacataaaaa ccgaaatttt caagtaacca aaataagtaa
     2041 tttttcggaa tttattgagg aagtatagaa ataatcagaa aaacacccgc acctgaacat
     2101 tgccggtgtg aatgaccgga ttgtcttcgc tggcgcgtcc atcgtaaata aaattcaact
     2161 gtatgttttt tcccaacgtc cgttgaatgc tgagattcca ggtaaagttg cggccgggct
     2221 gcagcccatc tagcatgata taacccaggt ttgtgttcag atcgccgttg aaactgatct
     2281 gaagcatgtt gaattgaagc atagcaagac ctttgcccga tttgctgaat ttcagctctg
     2341 tgcctgcttt gcggacaata gctttttcca ccgacagcac attgtttttt tcggaatact
     2401 ggaacagcat agtccagcgg aaggtggtgc ctggctgcca cgacagcgat ggtttgatcc
     2461 cggtttcttc gatgctgcaa ttgttcgatg tgaaatactc cgatgtgaaa tcgcgcagac
     2521 cgctgatggc cgacaattcc gttatccagc ttttcaaaaa cgatttgctg atttttattt
     2581 catcctgcct tgtctgccgg tcttcgatgc cattggtaaa cagtccccgt gcgcgattgg
     2641 cagaattgat ccagttgatg gcgaagcgcg aatcattggt cttgaaagtg agaacattcc
     2701 gccaggtggc gttctgagtg acaagcagcg aatccggacc ttctgaagta aatggcagat
     2761 acatctccga agccagatcg gatgaggttt ttttatcaaa tgaaaatgcg cttctgtttg
     2821 aaaattttga aatcagcttt ccggtgaatt tcttttttct gagtatatct gccggtgata
     2881 tggctacatt tccactcagc tgcgatgaaa aaaccctgat gtaatcgttg gttggcgtat
     2941 atacccgaat gaaattcgct tcatcgctga aaaccgctgg ttcaaattca ttcagctcac
     3001 ggatgttgtt tccgttgtaa tcgttccatg cataaacacc ttgcccggga gcgacttcga
     3061 gatatgaaaa ttcttttttt gcctccatgc cggaacccgc ttcgtaggcg gcccccagaa
     3121 caatgaatct ttttttcagg cgaccgctgt attcaagacg acccagcaga aattcatcat
     3181 ttgaaacatt ccctgtttta taaatcactt cgcgataggt ggcacgcaaa agcagctgat
     3241 tattttcagc aagctgtccg ctgctttcaa agccgatatc agtcatgtag ctctggggaa
     3301 ccaatcggtt gctgtcagaa atattgtctt cgcgatactg ataaaacaat ttggctgagc
     3361 gcatggaatc gttgtagccg gtgtacattt tagccagcca ccagtcgaac gatccggaat
     3421 gcagactgtc ggtggctgca ttgtgaatga gttttgactc accgttggct tcggctccca
     3481 gtgcgaagtt tccaagtttc tgctcgagct tgttttgatg ttgaacaaag tctgttttat
     3541 tgaaatgagt cggtgaagaa agataaactg ctgcagagcg gaatgaagtt gacttccagt
     3601 ttttattgat ctgcagcgaa ttctgataag aggcgttttc gggattgcgc atgaaaaacc
     3661 cgctgctgag gcggaatttg tcggcttttg tgttttgaag tacaacatcc gcaagcccaa
     3721 aataagtgtt accgcggttt tccatgaggt caagattcca gtcgcggtca aattccagac
     3781 tgcgaaaacg gtcaatggcg ttgaatgtgc ggtctgtaat ttctgtccgt atattggttg
     3841 tcagcgacca tttttttatg gtgtcgttgc caattttctg cagattgcgg aaatgaatgg
     3901 tcagtgcatg accgctgttg tctgcatcat taatcgaaga aaagagattg aaatctttgt
     3961 tgctgacggc aaattcagca tgcagtgcag tattcttgct gaaatcatat ccggcgcgga
     4021 atgcgtacat cgctgttttc ttgggcgtga tgagcatcat gactggttcg tactctccct
     4081 gaggcacacc tgcaacgggc ataacccatt tgaaaacacg gccgttggcg ctgcttccgg
     4141 tctgcacata attgccgttg tttttaccga catacgaaaa agttagcgca taaagagctg
     4201 aatcagcatt ggtcgaataa acaaaaacac tgtcatatcc aagcgtatca atcatggcat
     4261 acatgactct ttctgttgtg aaacctatac tgtcaacacc cgatgaatag gcgttttgta
     4321 tggagtcgcc gaccagcgac ataatcatct tttgctcgtc agacaggtct tgctgtattg
     4381 tttggttttt cagatctgat tctttataca aactcagtcc ggtagtaaat ttccctgatt
     4441 tgtagccaat ttcagaagta atcagtgagc gtgaataatt tttgtcggag tactcaaatt
     4501 cagccacaat gcgtgatttg gaagtaatgg ttacttttgt ggtgaacgta agttcggctg
     4561 tgttgtagtc aatgatatag tcgttgtctt cgccgcgtgt aagctgattt ccgtcgatgt
     4621 aaatcttttc gctgcctgcc agcacaataa taaaagtctc gttgttgtta cctttcagtt
     4681 tatacggacc gagattgctt tcaatcgcat tgatttcctg gcgggtgtaa cggcctttac
     4741 caaatgagcc attggcacgc acaaacaact cgtcgttttt ggtgagtccc ggcgaaaaag
     4801 ttgtctgaaa atcggcgcct tgtgctttgc ggctgtattt caggaaatag ctctgtggcg
     4861 ctgcttgcac ctcgatatcg ccagctgtaa aaatattttt ttctttcgaa agccggatga
     4921 atattttgtc gaattcctga atctgctgtg tgtttccttc tggctgaatc ggaatattgt
     4981 tgtcggaaat ggcggcttgt atttcaagat cattgctgag cttaccgttg agcatcaggt
     5041 tgagcgagga gctgagcgag aaatcctgcc ggttgcccac gctgaatccg cgggtgatgc
     5101 ttcctgattt gtcgagtccg tcggtttcga gccatgagcc ggagctcccg ctggctgttg
     5161 catcgaaagg attgtaatag ttgtttagct ccggaaacag cagcgtggtg tccttgtgtt
     5221 tataaggttt ggagaggttt acactcatgg ctttccactc gattttcaat tttccggtcg
     5281 caggaaattt tgctttttga aaataaagta atcctttgct gatgtcgaat cgaaaatacg
     5341 aggtgtcagc gatcgtacca tcgggtaata ttatttttat atcagccgga tcgaccgtaa
     5401 aggaatcgag ttgaatggaa tccggcaccg ggagctgcag cgaaagcgat ttcatgcccg
     5461 gtttttcctg agcagtcagc tgcacaaacg cagtcagaag gacaataagg ccaatgattc
     5521 gccgcatgtg ataaggaacg caaacaaaga taatctattt taagttgcca gtaatcagtg
     5581 gtcagttgtc agttgccagt ggtgagttgt gagtctggtc gagggtaagg gtcacacgtt
     5641 atagacgcgc tcccaggttg aagttaagga gttttgttat agctgacagc ttcagctgtc
     5701 agtacaaaat tagtgggaaa atgaagccgt ccggtattgt agcccaatat gaattatttt
     5761 ttttgcaata aaaaaatctt cgtatatttg caatccgaaa attggggcat tagctcattt
     5821 ggctagagcg tttgactggc agtcaagagg taacgagttc gactctcgta tgctccacga
     5881 gggaacctgg gttagccggg tttttttgta tggaaaagaa tacattattt ggattattgc
     5941 ccgaagaaat tgcggacatc attgaaaagt acggagcccg ttcattcact gcaaagcagc
     6001 ttgcagagtg gatgtatgca aagcgtgtct tcagctacga tgacatgagc aatctgccca
     6061 agcaattgcg cgaaaacctg aaaacggatt ttcctgtcat tctgcatcca tactcaaatg
     6121 tacaggtttc gaaagacgga acgaaaaaat atttgtttga atttgagggt ggccgcgtag
     6181 tcgaaaccgc cgttatcccg gatgatgacc gcttgaccgt ttgtgtgtcg tcgcaggccg
     6241 gatgtcgcta tggatgccgg ttttgcgcca ccggagccat tggttttctt gggagcctga
     6301 ctgcaggcga aattcttaat cagctcatgg ccattgatga atacgataaa attaccaaca
     6361 tcgtgtacat gggtatgggc gagccgcttg ataactggga agaagtgtac aaatcgcttg
     6421 aaattctgac ttcggagaag ttgatgaata tgggcgccgg ccgcatcact gtttcgacta
     6481 ttggtattca tgagcatttc aatgagctga ttgagaagac gcgtgtcaat attgccatca
     6541 gtctgcacag tccttttcct gaagagcgcg cacacatcat gcctgcacag aattcaaatc
     6601 cggtgggcga aattctggac acaattgctc atgctgatat cccgcgccac cgcaagcttt
     6661 cattcgagta tattcttttc aaaggcatca atatgtcgaa aagtcacgca tcaaaactgg
     6721 ctgatctggc catgcgttgc catgcgcaca ttaatctgat tgcctacaac gcagttccgg
     6781 ggctgccttt cgaagctcca gcccaaaaag atgttctggc ttttcaggat gaattgctga
     6841 aacgaggagc cagcgccaca ttacgccgct cccgtggttt agatattgcc gccgcctgcg
     6901 gaatgcttgc cgggaataaa cttgaagaat catgaacaat ccgtgcattc tgacggatca
     6961 agaggaacaa ggaaatttga aggttgaagg atgttctgta ttaagacaat tgttccgtat
     7021 tgaattcagc atagtttact gaacatcgtt cactactcac tgctgtagat acagctttgt
     7081 cattactttc ggtttcaaat gtccactagt atgtcacctt taaactttat aaactttatg
     7141 aactttacaa actaaaaaca ccattgggcg cgcgtttcct gcttgcagcg ttttacgttg
     7201 acgcgtgacc taataaaaat gtacaaaaat gtccacatca attcttgacc aatatctcaa
     7261 caacctgaaa ttcattgtcc tcaccgaccg cgaaacaggg caggcaattg aaatttattt
     7321 cgattacacg ttgcccaaca tgcacagtga aatttacggg caggctgtga gcgagtatgc
     7381 tgcaaaaggc cgccgcatcg gcatttcggg tggtggacga attacaaaaa ttgacaatta
     7441 tattgtactg cacagtcgct ccggcaatta cggccgattt gaagaagagg acgcactgcg
     7501 tcttgcaccc ttgcatccga tgctctgtga tcagggattt gtttttcttt caatgtcggg
     7561 ggaagacaat gccatggtgg tggtgaggga gtatgaggcg acattgaaat aggcacaagt
     7621 tgggtatagt atttacttat cacgcatgtg actgttatcc tttacaaaat tggttctgta
     7681 acagatagcc ggagtctgta tatcaccgac acaataatca gactcagtat tgccgaaaag
     7741 aagcaccaga ccgacacgaa gtaatcgaga tagaaaatgc cagtaacgag ataggcaata
     7801 aagacagaaa caccaaacag cttcatgcct tttacagaag aaaacatcag ggatccgatg
     7861 gtagccatgg catagaaaac gctgacgatc ggtgtcataa taacaggata tccgaaagtg
     7921 tagaagatat gacactcctg aattacgcca tctaccggaa aaatcatcat gcaggacaga
     7981 aaatacagcg acaccagaat tccggttgcc aacatgattt tcagtatttt tttctttttt
     8041 tcgctggttt caagtttcaa aacagcgaat ggcacccacg ttggccagaa aatctgtgca
     8101 aaaaacaaaa acagataagt acagggttct ttaaatatgg catattcgga atttgaaagc
     8161 gacagccaca gaaatccttc ggcaaattgc tgtgctgcaa aaagaagggg aatgcctgca
     8221 aatgggagct gctgtggttt tgtggttttc tttaacgcta ccgcgccgat ggcagtcagt
     8281 actgctcctg ctgcgaaact ggctgtggct gagaaacaca taaattaaaa gatttaaaga
     8341 agagttttaa ttctcatcaa aagtactacg aattttgatg caattttttc tcactgaata
     8401 tattaaaaag gtgtaacaaa gaattaatac ttgcaatcaa tgcaataaat ataattctcc
     8461 actgtaatca tgttgcagtc cgtcagtgaa ttccgcatgc agataatagt agtaaagccc
     8521 gcctgaaatg ctacttccgg gattccagca ggtatatatg tttgtagttc tccacatctc
     8581 aattccatcc tggtcggtca caatcagcag gcatttcgcg acatcgtcgg catcgatgca
     8641 ccacatttca ttatatgcgt cattgtcggg tgaaaaagaa tttggaatgc ggatacgctc
     8701 atcccagtta tggtcataat tattctcaca cgaataaagg aatgagaaag caatgatgat
     8761 aaagaagaat tttgctttca tgagatcaga gattaattga aaaaacaagc cgtgattgct
     8821 gtaaagaaaa ttcatctgaa acgggcctta taaaagcaaa tgatgctact ccgacagaaa
     8881 ttttctttcc ggaatacatt agcataactc cgccagattt atttgttgag agctgaattc
     8941 ccagacaaac gcggtgaaac tgataggtcg attcaatatt gaaaatgtga tgttgaagct
     9001 gattcattct ccatcctgtg gcgattgtgc ggaatccgtt ccgggaatca ttgtgatatc
     9061 ctgctgtaac cctcattccg cccgtgtatt cattgtatga aacaaagctg atattgggtg
     9121 tagtcaggtc ataaaacctt gccgtagcaa acatgtgcgg tgctgtaaaa accagatgtc
     9181 cgtgaaaggt tgcaacagca ttgcttaact gatctatcgg cttaacctga tttgactcaa
     9241 ttattgtgtc tggaattgtt agctgatcgg gaaagacaag actctcccag tttaattttc
     9301 gtgcaagcac ctgaaaggac attcctgctg ccaaagaata atccggattt ttcaaaaaat
     9361 cgtacagtac gcttattcca ccatttgaag ttttgtacat gtatgatgca gtgtaattcc
     9421 agtccgccgt gtaggtgaaa cgcgattttt ttgacggatt gaaacagatt tctgcatcgg
     9481 aattgatata aaccgatggc ttgtcaactg aaaaaccggg tccggccgat gctttaaaaa
     9541 aggcctctcc gggattctgc ggaaaaacag aacaggcttc acccggaaac aaaaccggca
     9601 cccggagcga atcctgtgcg gtcgataaaa atggaatcaa tagaaaaaga cagatgtatt
     9661 ttcgcataaa taacatttta ttgccaacaa atataaacga taatgtgatc caaatcaaaa
     9721 tatttttgta acaaacggat tttccttctt gcgttgtatc acaaattttt ctgggcgcgc
     9781 gtctcacgct tgcagccacg taaaacgcgg caagctgttt cacgttgacg cgtgaccact
     9841 ctcctctaca ggtgctgcgc gcgttttacc ccgggctccg ccatgggcta aagtattttg
     9901 cgccttcagc gcatctgatt gtacttcgta gcttttttga cggcttcgcc actgacaact
     9961 gacgactggc tactgaccac tgacaactga acactatttt tgtatctttg ctccgtattc
    10021 aataacaaat gaaaatttct gcttcgctat atagtgctaa tgcaaccgat ttgcgtgaat
    10081 tggtgcgtga actcgatgcc tatcagtccg attggtttca catcgattgc aatgatgatt
    10141 catcggtttt tgaggatatt cgcgaaatac gaaagtattc tgatacgcca atagatttgc
    10201 atatcatcag cccggagccg cataaattct atgatgtgat tgtggaaacc ggcgttgaat
    10261 atgtaaccct gcaatttgaa accattgaaa actggcatcc aatgccggat tcgtataagg
    10321 gtcagcttgg aattgccatc acaaccgata cggatatttc tgttttcgat cgttttgcca
    10381 atcaggcctc ttttattttg ttcatggcca caacgcccgg aaagagcggg gaaccgtttc
    10441 agcaaaagaa tttcagaaag atccgcgaat tccgaaacaa atatcccgac aaaaagattc
    10501 atgtggatgg cggcatcaat gctgagctat catttattct tcgcaacatg ggagtttcgg
    10561 ctgttgttgt tggttcttat ttgttgcggc aggaatttat tggttcggct atgatgaagc
    10621 ttcgcagcaa caccggaagc gaatattatg tcagcgattt catgctgaag cgcgatgaaa
    10681 tcccagctgt aacaaagaat aattattcgt ttccacaatt actgaaagtg attgatgact
    10741 ataaacttgg ttttgcaaac attgtcgatg aaaatgacac ccttttggga atcgcatcca
    10801 tggccgatgt cagaaaggcg ttgctgaaat actggcccaa ccttgatgac cttaaagtgg
    10861 aggatgtgat gaatccgcat ccggctttga ttcaaagctc aaacactgtt ttcgatatgc
    10921 ttgattatgt gaataatctt tcatttccgg tattgttttt acctgtgacc gatgacaacg
    10981 gaaaacttgt aggaacagtg aaattcaaca atctcataaa aggcgaatta tgatcattca
    11041 actcagaaaa aatatttcaa accctcagat tgatacactg gcagcggctt acgaaggtgt
    11101 cgttgtcaat tacaactcga atgttctggt ggttacacct tccgcaatca aaaacattga
    11161 ttcaaagttt gacgacatcg ttgagaaaaa ctgggtgttg gattccgaca tacagctttc
    11221 ttcaaggaaa tttaaaactg aaaccagaaa aatcagtatt ggcagatata caagcggaag
    11281 tgcgcagact ttactgacca tcggtccctg cggtgtcgaa ggtgaggaaa tgatggagca
    11341 aacggcaaag ctcattgttg agtcaggact gacaagtatt cgtgctggcg ctttcaaacc
    11401 ccgcacttcg ccttatagtt ttcagggaat gggcgaagat ggattgaagt tgttggcgaa
    11461 aatgcgtgag aaatatggat tgtcagttgt taccgaagtg cgcgacagta ctcatgttca
    11521 gcttgtgctc gattatgccg atgtaattca gattggtgcc aaggcaatgt acgatcatgg
    11581 tattctgcgc gcctgtggcc gttcacagaa gcccatatta attaagcgcg gattcggaac
    11641 aacattgcag gagtttgtac aggccgctga atttgtgttg tcgggcggaa atccgaatgt
    11701 cattttatgt gagcgaggca tcaggacatt tgaaacgaaa tcgcgattca cgctggattt
    11761 gtgtggtgtt gcgtatttga aagaaaatac gaatcttccg gtaattctcg atccgagtca
    11821 tgccatggga tatgcatacg gagttcctga cctggcgcgg gcatgcatgg ctatgggtgt
    11881 tgacggtttg ctgattgaag tgcatccaaa tcctgcggtg gcgaaatcag atgcatcgca
    11941 gcaattgaat catgaaacat tcagaaattt gtttgaatca ttgaaaccca ttgctgctgc
    12001 ggttggacgg actattgttt agtagttctt tatatctctt cgacggtgtt tatcctgtag
    12061 aatgcagggc tcagagtgac agcaaagaca aagagtaaat attgaaaagc aaaccatgaa
    12121 aacattcatc agcaatctga aaaaaataat ggagaccgaa ggtcttgatt tatctgagct
    12181 ttcggccaga tgcgggattg acaaaagtgt catgcaatct ttcctggatg ggaaaaattg
    12241 gccgaagccc tcggaattgg ctcagatggc tgattacatg agcttgcctt nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aattggccga agccctcgga attggctcag
    12421 atggctgatt acatgagctt gccttttgaa agtcttttat gtcctgctgc agaaattcct
    12481 gctgattttg aatgtaagct gcttgccatc gatatcgacg gagttatgac tgatggcgga
    12541 atgtatgtca cttccgatca gaaggaaatg aagaaattca atacccgcga cggcatggcc
    12601 atgagaattg ccgcccggct cggcatcgaa gttggcttca tcagtaatgg acagatggaa
    12661 gggctgattg gttatcgtgc tgaaatgatt cacgtgaagc gctggtacgt gggcaatgaa
    12721 aagaaacttc cagtgcttga aaactggtgt cttgagttgg gaatcagtct gtcgcaggtt
    12781 gcttttatcg gcgatgacat caatgatctt gaggtgatgc agcaggttgg tttgagtgct
    12841 tgtcctgccg atgcagtgcc tgccattaaa gctgtttcaa aaattgtttt gcagaccaag
    12901 ggtggacatg gctgcgtgag ggagttcggc gaaatgctgg gatggacgcg gtagattaag
    12961 tattcagtaa tcagtagcca gtagtcagtt tgaaatttaa aactgaccac tgactactca
    13021 ccactggcta ctattataat tttttttgta cattttccga attgaataat atttctattt
    13081 ttcaagtata tcttgggttg tattttttct tacctttgca aaaaatttga aatatggact
    13141 ccaaagtggc tctgttttct ggtcaggcaa ctcgtccttt agctgaaaaa atcgctgaat
    13201 cctacggtgt taagttggga aatgtagaat tgatgcggtt ttcagatgga gagtttcagg
    13261 tttcctttga gcagacattg cgtggatcag agttatttct gattcaatcc acgatggcgc
    13321 cgagcgaaaa tctttttgag ctgttgttga tggttgatgc agccaaacga gcttcagcgc
    13381 atcacatcaa cgctgtgata ccgtatttcg ggtttgcgcg tcaggatcgg aaagataagc
    13441 ccagagtggc aatcggtgca aaaatggttg ccaacctgtt gatggctgcc ggtgttgacc
    13501 gggtgattac gattgatttg catgctgatc aaattcaggg tttttttgac atacctgtag
    13561 atcatttatt tgcatcttca attttcattc cttatctgca aagtctgaat ctgggcaaca
    13621 acattgtgat ggcttcgccg gatacaggcg gcaccaagcg tgccaatgca tattcaaagt
    13681 atttggattg cgatatggcc atttgttata agcagcgttc gaaaccgaat caaatcagca
    13741 acatgacttt gattggtgat gtagatggaa gagacgttgt attgcttgac gatattgttg
    13801 atactgcagg aacgctttgt aagtcggctg aattgatgat gaatgccgga gcgaaaagtg
    13861 ttcgcgccat gtgtacacat ggtgttttca gcgggaaagc atatgaaaat attgacaatt
    13921 ctgttttaac agaactaata gtaaccgaca ccattaccaa ggatcacaaa tcatccaaag
    13981 taaaggtgct ttcagtggct gaattgctgg cggatgttat aaaacgaaat ctcacatgtg
    14041 agtcaatcag cactcacttt aatttaaaca ctctaatcta aaacaaacaa aatgagaaca
    14101 gtatctatga gcggttccct cagagggaac gtagggaaaa aagatgcaaa gcgtctccgt
    14161 aacgaaggcc tggtgccatg tgtgctttac ggacaaaaag aacagcttca tttctttaca
    14221 caggagaaac agttcaagga tgtgatttac actccgaatg catgttttgt tgaactcaac
    14281 attgatggaa acaaacacat ggccattctg caggaagttc agtatcatcc ggtttctgac
    14341 agtattatgc acgttgactt ttttgaattc gccaacgata aacctgtttc actgactatt
    14401 ccgattagtt taacgggaac atctcctggt gtactcaaag gaggtaagtt gcagcagaaa
    14461 gtacgtaaag tgaaaattac cgctttgcct gaaaaaatgc ccgaaaaatt gattattgat
    14521 atttctccgc tcgatatcaa cgatgcggta agggtaaaag acgttgttgc tgctgattat
    14581 aaaattaatc ttcctgaaaa caatgttctt attatagtaa ctcaaacccg aaatgttgtg
    14641 gatgaaactg ctgcaactga agatgctgct gttcctgcag ccgccgctgc tcctaaaaag
    14701 tagtttttcg gttatattga taataaaaaa ggcaggaatt tcctgccttt tttaatttta
    14761 gtaatcctcg aagtgtttca aatttttcga gcgatttatg atactaatcc atcagtaaaa
    14821 aatacggcag acgagcctga acaccgctca tttgcgttgc ttttttccaa gcttcgtaca
    14881 atggtgcaaa ttctcccagt tcctgctcaa tgatttttcc gctttcaact tttgcttcgc
    14941 tgccgaaatc ttccatgagc gttgccagga aatctttctg aacaggaagt tcttcaacgg
    15001 aatattctgt aagattggcc gcctgtgctg cataggcaat ggcatcataa atgccgccca
    15061 tttcgtcaac cagaccgaga cgcatggcgt cggtgccgca ccagatacgt ccctgcccaa
    15121 tgctgtcaac gtcttcgggt ttcattttac gtccttctgc tacatgtttg atgaacgtgg
    15181 aataaatatc ttcaatcata aactgcagcg ttgctctgtc gtattcattc agcggcttca
    15241 tggtgccgat gtaatcagaa tgctggtttg ttttcacttc atcaaaggta attccaagct
    15301 tttcgttgaa taatttctgc atgtttggaa tcatgccgaa tacgccaatg ctgcctgtga
    15361 gggtggtggg ctcggcaaaa attttccgtg ctgcgcaact gatgtaatat ccgcctgatg
    15421 ccgcgtaatc gcccatggaa acaacaacgg gttttatttt gttggccatg taaacctcgc
    15481 gccagatcat gtccgatgcc aatgcgtcgc ctcccggact gtttacacga agcacaatgg
    15541 ctttgatgct tgaatcggcc gcggcttctc ggatgacttt cgacatgtgt tttccgctga
    15601 tggttccgtg ctgatatttg ccattgttga tttcgccaaa agcataaata acagcaatgc
    15661 ggtctttgtt gtaactcagc agtttttctt tgattccttt ataataatca ttcattgaaa
    15721 tataaggaat atcgcctgtt tgaactgatt ttgtcagaat cagtttattc agttcatcgc
    15781 gataaatcag actgtcaaca atgttgtatt tcaatgcgcc ttcggcgttg agtgcaaaaa
    15841 gactgtcggc caccatattc atttttgcag tatctgattt gcggctggct gcaatttctt
    15901 ttaccaggtg accccagatg ccatcggcaa aagtctgcag ctggagccgg ttttcatcac
    15961 tcattttttc aagcaggaat ggctcgacgg cacttttgaa tttgccgtga cgaatgattt
    16021 gaacatccac attcagcttg gcaagcgcgt ttttgaagaa catggtctgc atgtaaatac
    16081 ctttgaaatc aacagcgcct tccggattga gatatatttt atcggcagca gtggccagat
    16141 aataggcctt ctgcgaaaaa tcatcaccat aggcgatgac aaatttgcct gaggctttga
    16201 aagtgtcaag ctgatttcga atggcttcgg tggtagcaaa acctgcatta acatcgctca
    16261 ggtcgagcag gattccgctt acgcgctcat cggtagctgc atatttaatg gtgcgcagaa
    16321 tgttgttgag ccccatagca gtttctgatg acatggaacc gaaatccata ttatcgaatg
    16381 gattatcaga cgtacgttct gtaatggctt tgttcagttc gatcacaaga acggatttgt
    16441 cctgaatttt cacttcttcg ctgccacccg acatgaggcc ggaaatcaca gcaaacatga
    16501 ttacaaacac aaccagcaca gtaatcagac agccggtcat ggaggcaaac atcattttga
    16561 agaattcttt cataggtttt gttttttatt tgtgtaaaag taagaatttt cttttcgtca
    16621 gcaattttca ttcgacaagc cttgaatcag tatatacaag cgcctttaaa atctacgtat
    16681 cgcgctttgg aagtagaaag tttttaaagt tcttaaagtt gaaagataac gtttgaaatt
    16741 ttcactcacc actgaccact tcaagttatg agttatgagt gataagttat tagttgattc
    16801 gcactcacaa ctggctactc acaactgacc actaagaaat gcctttcaac tttcaacttt
    16861 acaactttat aaacttcaaa gaactggcgg caattaaaaa aggtaccgga agaaatcctc
    16921 gccctccgat acctttttgc aaaataaacc aaccatgcat aagacggagg gtgttaggga
    16981 tttgttacag gccgggtgaa atattttctt ttttgtaact ttgaataaaa attacaccat
    17041 ggaaaaactg tcatctttat tggaacccgg ttttgttttc ggggagaaaa tgcgcgtatt
    17101 gctgaaccat gcaaaagcta ataatttcgc catccctgct gtgaatgttg tcggcaccga
    17161 ctcgataaac gcagtaatgg aagcagctat gcaggccaaa gcgcctgtta tgattcagtt
    17221 ctcgaatggt gggggacaat tttacgcagg caaagcactg cccaacgatg cgtatcaggc
    17281 cagcattgca ggctcggttt cgggtgctga acatattcac cgcgtggccg ctatgtatgg
    17341 tgtgccggtg attatccaca ccgaccatgc tgccaaaaaa ctgctgccat ggattgatgg
    17401 tttgcttgat gccggtgaaa aattctacgc acagcacaag aagccacttt tcagcagtca
    17461 tatgctcgat ctgagcgaag aatcgctgga ggaaaatatt gaaatctgca aaaaatatct
    17521 ggctcgtatg tcgaaaatcg gcatgacgct cgaaatcgaa cttggtgtta ccggcggaga
    17581 agaagatggc gtggataata caaatgtcga taattcaaag ctgtacacgc agcccgagca
    17641 tgttgcctat gcatacgaag aactgatgaa gatttcgccc gatttcacca tcgcagcttc
    17701 gttcggaaac gtacacggag tctataaacc aggcaacgtg aaattgatgc cggtgattct
    17761 gaaaaacagt caggattata ttcagaataa attcaacaca caggaaaaac cagtgaattt
    17821 tgttttccac ggcggtagcg gttctgcacg cgaagaaatc cgtgaggcca tttcctacgg
    17881 tgtgattaaa atgaatattg ataccgatac tcagtgggct atgtgggaag gtgtgatgaa
    17941 tttctacaaa aagaacgaag gctacctgca aggccagatc ggcaatcccg aaggcgaaga
    18001 caaaccgaat aagaaattct acgatccacg cgtttggttg cgcgaaggcg aaaagtcaat
    18061 ggtgaagcga cttttagtgg cctttgaaga tttgaatgcg gttggcactt actgagagaa
    18121 tgctttgaat tagcagtgag cagtgattgg tgagcagcga actggttaac ggttatcagt
    18181 taatggttaa cggataggtg catggacaat tcagttagaa tagcggtggc agcttttgtt
    18241 atcaggatgt gattgtctga attccgctgt ctgtctggtt ttaagtgata aaaagttatg
    18301 cgcagatata tgtctggtaa atacaaaaca atacttgtta ttctctttat ggcatgtaaa
    18361 ggttttgcat ttgcacagac tgttttattt catgaatatg agatgaccgc cagctcgtgg
    18421 tctttgctgc ggtggaatgt cgatgataca accggagtca aaaacatttt aagggagaca
    18481 gttgatgctc agggtagagt ttcatctttg gaatttctga aaaacggaaa aattttcacg
    18541 gataaaattt gttatctgcc ggagaaagtg gaatatgagt ataccgattc aaccattaca
    18601 gaattgctat attcagatgg cgccctgatg taccagaatg actgcgaaat gtggtttaag
    18661 actgtctatc atttggattc aaataacttt gtgtttgaaa aagaagtttt ttcccgaaag
    18721 gatacatctg aaatgtcacc ggataaaaca cgttggttgg atgaaaacct cccggaatat
    18781 cagcttgaag agttatctcc ggagagttat tttttcgtca cgtactacta ctattcctgg
    18841 gcaaagttga atgggctgat gcctgtttcc gttacgtatg acccgtcggc tttcgatgta
    18901 tatgactctg aaattccagc atggaataaa gtcattgagg gaattgaaaa actcaggcag
    18961 caagctgaaa tgaaatagtg tttctgttgt ctcaggttgt tgtatttcct gatgagtgcg
    19021 atagctgact tatattattt gctttttgta ttatagtgtc agacaatcca tctgtctgtc
    19081 atgataatta tcattgcagc tgacacatag tcgcgatttt tgacgtgaaa tgattgattt
    19141 gtgtcgggga atagtatttg ttgacggcaa acatctaaaa atctgcatat gaaatcttct
    19201 gttaatggaa agtggctggg taatatggct tttgaagcnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnncc tatgtgtatt cctgtgtatg ctgtgatgga agatctggaa
    19381 aacgagtaca gtcatgtgaa atttgccgac atgttgtttg attcacccga agcgcatgta
    19441 atccgcaacg ctcctgaagc gcgtggtttt atggggctac cgtttgtgat gtattataaa
    19501 aacggcaaag ttgtaaaagc ggtaagcagc attcaaagcg aagagcaggt gcgtgaagcg
    19561 ctggaagaaa gttttggagt tgttgtaaaa taattcaaat gaacgagcaa aataaattcg
    19621 atgttattgt aataggtgga ggtccggccg gattaacggc cggcatttac ctgtcccgcg
    19681 cgaaattgaa tacgctcata ctgaatgtcg gcgtggctgg cggacaaatg attctgacgc
    19741 atgaaattgc caattaccca ggtgttgaaa gtatcagcgg atacaagctg gcatcgacca
    19801 tgaagaagca ggcgcagtct tttggtgcaa caattaaaac catttctgct ccacccgaag
    19861 ttgtggatga aggcattttt aaaatcgtac gtgcaggcga aaatacatac agcgcacact
    19921 cggtgattct tgctcccggt ggtgctccac gcatgttggg tgttaaagga gaacaagaat
    19981 ataaaggtgt tggagtaagc tattgtgcca cttgcgacgg agaatttttt acgggcaaaa
    20041 acattgttgt tgttggtgga gggaactccg cactcgaaga agcagtctcg ctttcacgct
    20101 atgccgaaac ggtaactatc gtgcatcagt tcgatcattt tcaggcattt ccggcctatg
    20161 tggaagaagc gaaaaacaat ccgaaaatca aattcatcat ggaaaccgaa atccgtgaat
    20221 ttgccggtga tggcaaagtt gaacgtgtct tattgcacaa catgaaagat gaaaaagaag
    20281 aatggtttga aactgaaggt gcgtttattt tcattggcta tcaaccgaat acaacatttg
    20341 taaaagattt gctgccattg aatgaacgtg gtgaaattgt tgtaaatgca gatatggaaa
    20401 cggctgtaag cggcatattt gctgctggtg acagcattgc gaaaaaatac cgtcaggtaa
    20461 caacggcggt gggtgaagct actgtagccg ctttggctgc tgcagcacgt acctgggaaa
    20521 taaaaagtaa tcattaaata aaatttttat gcctgtctgg cttattgtac taatcagtgt
    20581 tgtaggactt ttcattttgt tgattgtccg aagttatctg aaacttaaaa acgcttccaa
    20641 tgacgctggc agcgagcatt tgctgcatct gaccgacgtt gattttcaga aaaaaatttc
    20701 aaatggagta acattggtcg atttctgggc gggatggtgc atgccatgta aactgttggg
    20761 accgaccatc agtcagcttg ccgatgacaa tgccggagaa gtaaaaattg cgaaactcaa
    20821 tgttgaagag tcgcaacgta ccgctgcgaa gtatggggtg agcagtattc cgaccgttat
    20881 tatttttaaa aacggcaaag aaatcaatcg cattgttggt gttaagccga aacaacatta
    20941 tcagaatctg attgataaag ccctgaaata attatcgcaa attcgtgcca gtggccagta
    21001 ttcagtagtc agtagccagt ttgacttcac tcacaactga taacatcctt gcttgtcccc
    21061 gtagccctga agggcatgcg ggctattaat atttatcgcc ttccggcgaa taattagtga
    21121 gctgcgcagc tttttttatg ccttcggcac tcaccactca caactgacca ctggcaactg
    21181 accacttcga tcattggttc atctcgccgc gcagcggttt ttgcagcatg gtggcaagtt
    21241 ctttatcctt gtagccctga cctaataaaa acatagcttt cgctattgct gcttccgttg
    21301 ttatatcatt tcccgaaaca acgccaaggt tcatcaaagg cagactggct gcgtatttac
    21361 ccatttctac cattcctcct ttgcattgtg tgacattgaa taatactttg ccagatgaaa
    21421 cagcttcgtg aatatttttc acaaagctgc tcgttgtggg agcattgccg gagccaaaag
    21481 tttcgagaat aataacatca agttcttcct gtagcagaac cgggcgcgtg tatgcatcgg
    21541 gcattccggg aaacagtttt agaatgccta cccgtggcg
//
LOCUS       DAOPKE010000050        20192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2821_length_20192_cov_12.484879, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000050 DAOPKE010000000
VERSION     DAOPKE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_20192_cov_12.484879"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKY63_07735"
     CDS             complement(<1..282)
                     /locus_tag="PKY63_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPF00540.1"
                     /translation="MADLKVNIGNLELQNPVLTASGTFGYGYEFEDFLDINKLGGIIV
                     KGTTLEPRQGNAYPRMAETAAGMLNAVGLQNKGVHVFIDEILPKIKHYQS"
     gene            complement(275..1078)
                     /locus_tag="PKY63_07740"
     CDS             complement(275..1078)
                     /locus_tag="PKY63_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPF00541.1"
                     /translation="MNKKSKALLPVLKNTKLNSKHFVLTLQLPADHPEIWPGQFVEVL
                     VEGSPETFLRRPISVHDVDEKNHTLDLLVKIVGAGTEKLSHLCEGDQVDIIYPLGHGF
                     SLARNNQKVLLAGGGCGVAPMLYLARKLRETGADCHVAIGGKTSTDILTADAYSELAQ
                     VAILTEDGSAGDKGMITAHPWFGADLKSFSKVYVCGPDPMMKAIGKLATDAGVYCEVS
                     LENLMACGIGSCLCCTVSTHEGNVRACMEGPVFDVTQLKDWNHNPVCHG"
     gene            complement(1071..1505)
                     /locus_tag="PKY63_07745"
     CDS             complement(1071..1505)
                     /locus_tag="PKY63_07745"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPF00542.1"
                     /translation="MDLSKIIAVSGKPGLFSLVSQTKSGMLVESLLDGTKIPVFATDR
                     VSALADISIYTTGEDMPLKDVLVAIFKNLNGEKAIDAKADKKEIIAFMDKVYPEWDRE
                     RVYPSDLKKLFMWYNILVEHNLIDDKPAEEAKDETSETPANE"
     assembly_gap    1671..1770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1771..2000)
                     /locus_tag="PKY63_07750"
     CDS             complement(<1771..2000)
                     /locus_tag="PKY63_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPF00543.1"
                     /translation="MKAFVFPGQGAQFPGMGKDLYDRFPMAKEMFDQANTILGFNITD
                     IMFGGTEEDLRQTRVTQPAIFLHSVILAKCMGA"
     gene            2218..2991
                     /locus_tag="PKY63_07755"
     CDS             2218..2991
                     /locus_tag="PKY63_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00544.1"
                     /translation="MMKPCCVLVLFILSYVVCAQSHQLGTSVSMIPLYSKLSESSGAT
                     VENAVSPYFTASIDYCFTDKNLGLHLSVFKGFALYSVEYSFTNKWVYWTGSSGSEGGR
                     IEHNTIMFKSKIPSKGIFAGLNYKVFQSDNSRHSLFLSESVRLESARYNYVIKDRQYT
                     QVINYVSDSTMTYVSYDSTNMLYSGSSYLDMNDEVSVYVCPGIQYQYKISDKFSINVS
                     AMMILGLGDNLATFKQCTIIPVGQIGFGCRYHFLVKRKN"
     gene            complement(3029..3808)
                     /locus_tag="PKY63_07760"
     CDS             complement(3029..3808)
                     /locus_tag="PKY63_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00545.1"
                     /translation="MRIIYFFICILFIIDCSAQQHSIGISPYSAFMSFKPRESSYPDK
                     SSGEPQKYMSYGIKFRYNYMENFLGLSAGMQFQYIPLDMKYNSHHEFADANHGGAYSI
                     DTYSTYIYQSTFFVTGLFAGFNVLLSADDDKNNRFMFSGIAQLNSIVGYKEFKDSVYI
                     TKHYYSYSLYPSYTESSSSTYSSAADVITLEPGNAPFLSLLTGLKYNHAFNNKFELDI
                     ELLFNFQSDYPLHLLQIEKIKPFVHFCAGINYRFSSNDQKR"
     gene            complement(4089..4472)
                     /locus_tag="PKY63_07765"
     CDS             complement(4089..4472)
                     /locus_tag="PKY63_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPF00546.1"
                     /translation="MSVIRIKTCSDSFEANIIMGKLKQAGIECFLTNEKFSTLMPHYT
                     GVMDAGVQVIIDEKDQTAALEILNETESEKVIACPHCKSLNVASGPGRKWAGKYMLLF
                     LSILFFFPFGNRKPRFYCRDCGREF"
     gene            complement(4518..4844)
                     /locus_tag="PKY63_07770"
     CDS             complement(4518..4844)
                     /locus_tag="PKY63_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00547.1"
                     /translation="MKAIIVFLTFFAFSIAAMCQHTVNPQAKEHVYYGIKLEQKEDWE
                     GALEEYTKALQIDNEYAEAYFYRGLLLINHGEEELKPYGCSDLEMAKHYGYTPASSYH
                     YSLCFQ"
     gene            4986..5516
                     /locus_tag="PKY63_07775"
     CDS             4986..5516
                     /locus_tag="PKY63_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPF00548.1"
                     /translation="MKTIVIYRSKTGFTRKYAQWIAEELQADVREYKRVTPEELHCYD
                     TIIFGGGLYVGGINGLSSFREFLPYLRDRKIIVFATGATPDRNETTQELREQNFSKEQ
                     QEYISFFYLRGGFDYSKLGLKDKFLMSLLKLKLRMTPESKRTPDETGMLAAYQNPADF
                     TKKKYMEELLELARQN"
     gene            complement(5548..6612)
                     /locus_tag="PKY63_07780"
     CDS             complement(5548..6612)
                     /locus_tag="PKY63_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00549.1"
                     /translation="MKAINLSPLFFLSIAIIISLFFGNKIQAQTYHPIVQSGSDTSLL
                     TAVDCDYFSYETFNTFILNDTIINGISYKNVKEFRHSYDFNPPYYPQYGMYDQYYIRE
                     DSASRRLFVRYLNQTEFLLYDFSLQVGDSIDLIVYPKIINNIITQTTPDWDTLRFYAT
                     AVFTVKNSVSDSLLPAILLQNSDFNDTISIIWIESIGALNNFFYCDRYLNIYEDENLP
                     YFINVSISLPPYRNSTHQKCKFVNKVNVFNINSILVSERSDEPCDYQQSISLSVEENT
                     ECFHDIVVYPNPAENELYFDSHLSDAFYFVYDSFGKQVLLGKTENSSVDISSLNPGFY
                     MVELIKNGKSFRAKFVKGQN"
     gene            complement(6628..9417)
                     /locus_tag="PKY63_07785"
     CDS             complement(6628..9417)
                     /locus_tag="PKY63_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00550.1"
                     /translation="MTTNGGSSWTDISSGLPFLPVNDLEILEGISGDDILFAATDIGV
                     YWKNGTADWQRFGTGLYNCLVDDVEINHLDNVLYAGTYGWGLWKCALPCSDETALLEI
                     STNTNYSTYTRMDRPILIKNGATLTVTNTLTMPEGTKICIERGGKLVVDGGIIKNGCK
                     SAMWGGIEVWGNSVASQLTAGAQGKVELKNGALVENATDAVIAIKKNTDGSFDWNYTG
                     GIISINNATFRNCNNSVSIYTYHNFNPSNPTVTTSNLSVIKNCIFETTDILIDNDGIP
                     YSFLSLIDVEGIGIFGCTFRNTRTDISNASDKGIGILSYDASFMVDDLCISGTSPCYN
                     YQSSIFANLDYGVKQINTNTNHIASINSAHFADNHHSAYFSGATGTVITRNSFNITRD
                     IGYGIYLDQCNGWQCEENVFTGTTSVNSFGIVVNNSGAVNNIIYNNTFSNLKYGIETL
                     DKNRGILATIGLKIKCNDFSNCSKDIYVTQYASCSPCGICKYQGANTSVSAPAGNTFS
                     HAGPSNQYSDFDNVFSSYPIIYYRHTGSTGNWIPYYFRNLTNSVTNFTYTKAAACPSY
                     LGGSGGIEKSTIVQRYDSEIDSLTNILNILVDGGNTAALSQEVSTATNSDALSLTTNL
                     LSKSPYLSDSVLAEAATNDNALNPYMLRDVLNSNVHGIKSELVQDAIINRSVPLPSNL
                     LNGIMSGLNMVSEFENLQSKIAILSDKRKQYINQQIRYWMADSTGGGNEPLIMFTQLC
                     NDWDIKAILLFKDSTAMISGLPNSEAYNSVSTLQWNLENSNRNIFSLTQNEKYLLNQY
                     SGNMDGFADMYALNMLRISGDTTYNEPLVLNSEGKSKIFPVSDPVSESYELFQVFPNP
                     AGNYIMIVLNEQSLNYNTCEISDSKGVVLRSEILPAGCTELYFDLSGIEDGVYFIGFV
                     SNNATKQLKKIVVNK"
     gene            complement(9407..10864)
                     /locus_tag="PKY63_07790"
     CDS             complement(9407..10864)
                     /locus_tag="PKY63_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00551.1"
                     /translation="MTKYIKVYRSNDKGNSWQIVFGTQNYSLTHCLTWKKKLLRDIEF
                     LPGTDDKVMISSDGYSYDDNGNLEHFQAELFFCENFQDQNPDWVDFDFRPLLTERISV
                     AVSQIDLENFYFMFSTQTDNDAQNKSDLFFGIYNANSLVQSEYIYYNSPGYQNSPFGG
                     VGYWKQEYVISDSDPNIQYIGGLTVTRANFNNYTFNNVFSQMHVDVRCSYFYQSTNGG
                     GFEDDVLFFGNDGGLTKIENDIAYNINGNGLCISQFYGVSDSETKAERVLCGAQDNGS
                     FVFDNGSLINTRVGDAYDGVFNSKDSIMLRTCGDGIGNNVNVLKSHTLGQTYKIALTT
                     SEFHLNDAPIVGNAIQSNTVYSCYHDIWKTTDWANNSLSSWSNISNFSTTMPQIANTE
                     RIQGFDVFDADENVLWVSFSNPTYSANVYNKLLKSTNGGATWTDITQNVPGYPFGYYA
                     ITHISIHPTNSSKVYITLGGDVFSIHPCKPSFCDN"
     gene            complement(10839..11681)
                     /locus_tag="PKY63_07795"
     CDS             complement(10839..11681)
                     /locus_tag="PKY63_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00552.1"
                     /translation="MKIKLLNRIFTGFLFLTLSINCHSQTDSIKVFNGWAFSANAANA
                     NYDSIKIEAENILGKNEYISSIPGSGYKDYIRWELFWRNRVQFPSYRDGGFLSNYAVA
                     REKWDSLCYINGDTFAENRTQGSLNWELVGPNGQPGTGSTNQNNGLVSAVWVDPDDIN
                     EIWIGSNTAGLWKSTDGGANWLNVNIFSQYYHGVKLITGVQYITANPNNPDEMYIATG
                     KSGYPELTYSSGLFRSTDGGNTWTVILSLSLTEIHMCSKIIVNPENGNQIAALVDDKV
                     YKSI"
     gene            complement(11850..13196)
                     /locus_tag="PKY63_07800"
     CDS             complement(11850..13196)
                     /locus_tag="PKY63_07800"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPF00553.1"
                     /translation="MTQTKDNSFYNAPERVVVADDAYEKGLEFWQKERHDTLSEDEQT
                     IYFMVDSIKKMPTFNTVYDIIDMFVSGYLNWGKFDIGPYASMLSFNEIEGARFRLGGR
                     TSEKFSKKIMPSAYVAWGTKDQKFKFGGGFLYLMNKKPRRGFQILFKHDMEQLGMSPT
                     GFREDFLLASFITRNPANKLTLVNELKAHYEHEWFTGFSNTVRFLHKDIFPVGDTVFN
                     FGEGTTREKITTSEITLEMRFAYKERYVYSDFIRTSLGTIYPIIQFKYSKGIKDLWGG
                     DYNYQKASFYFSDWFNVGNIGWSKYVIEAGKIWGRLPYPFLKMHEGNETYFFDETSFN
                     TMNYFEFVSDEWVSLYYTHRFDGFFLNRIPLLRKLKWREVVWGKGLIGNLSAANRNYS
                     EFPEGMHTLGKPYIETGAGIENIFKFLRVDGVWRLSYLDHPDIAKFQVMFGMQVFF"
     gene            complement(13193..14221)
                     /locus_tag="PKY63_07805"
                     /pseudo
     CDS             complement(13193..14221)
                     /locus_tag="PKY63_07805"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
     gene            complement(14299..14649)
                     /locus_tag="PKY63_07810"
     CDS             complement(14299..14649)
                     /locus_tag="PKY63_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPF00554.1"
                     /translation="MSKVKEFKEYRLKMNKAILEGDNKVIKRLFSVDTMAYDPGALNT
                     KTKEMLGLVSSLVLRCDDCVKYHLEKLCEIGTSDAEIHEIFGIAILVGGTIVIPHARR
                     AVEFWNELNKPDAL"
     gene            14785..16983
                     /gene="recQ"
                     /locus_tag="PKY63_07815"
     CDS             14785..16983
                     /gene="recQ"
                     /locus_tag="PKY63_07815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPF00555.1"
                     /translation="MKQIETDDIKGVLKTVFGFDKFKGEQEEIIESLLEGTDTFVIMP
                     TGGGKSLCYQLPALVLPGTAIVISPLIALMKNQVDNIRNFGTENGIAHVMNSSLNKAE
                     MALVHEDLLSGKTKLLYVAPESLTKEENVEFLQGIDISFYAVDEAHCISEWGHDFRPE
                     YRRIRPIVEAIGKNVPIIALTATATPKVQQDILKTLKLKDPKVFISSFNRPNLYYEVR
                     PKYKDVNKELLKFVKANEGKSGIVYCLSRKKTEELAEFLQVNGIKALPYHAGLEAAVR
                     RQNQDMFLMEEVDVMIATIAFGMGIDKPDIRYVVHYDMPKSLESYYQETGRSGRDGGE
                     GLCIAFYSIDDLQKFEKFSKDKGVAEQEIAQHLLLETISFAESSVCRRKQLLHYFGEN
                     YLHDNCHSCDNCLFPRDTFEAEDDVCLVLDTIEETQEQFKSKSIVDIILGSKSPSMKQ
                     LKAEKLEVFGKGGEKSERFWNSVIRQMLIMNFMAKEIENYGVLKITEKGKEFLNNPHS
                     IMLTKDRNIEEEKEEDGPSSGDGRSSGTDKALFAMLKDLRKQIAKKENLPPFVIFQDP
                     SLEDMAIQYPITLDELKNITGVGAGKAGKYGEPFLKLIAGYVEENEIIRPMDMVVKST
                     VNKSKLKVFIIQAIDRKVALEDLAVSKNLTFEELLDELERIVSSGTRIDLNYYIDENI
                     DPYHQDEIFEYFRTAETDSIDDALTELGENEYTVEEIRLMRIKFMSEIGN"
     gene            16993..17637
                     /locus_tag="PKY63_07820"
     CDS             16993..17637
                     /locus_tag="PKY63_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPF00556.1"
                     /translation="MEMNLNNQENSFVPDEKKKSCLRSPQMIFNIAAGVLILILLILQ
                     FYNPFSKENGIVVPDGGLNIAYVESDSIMTQYDLVDTLKGDLVKLNDSLDKDFTKRQE
                     GFQARVNLFQQNVQNGKIQTKDDYSRQESALAAEQEQLMNLRDSYLAQVQQKQIAMNN
                     QILDSIVSIIKQYPEEFPYDYVLDYNSGTGILYANDKLNITVKVVDRLNKEYNK"
     gene            17637..18065
                     /locus_tag="PKY63_07825"
     CDS             17637..18065
                     /locus_tag="PKY63_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00557.1"
                     /translation="MRSLGFFLFFSVIVFALISCGGDKSVRVVEKTYPDGKDNIVVYY
                     SNDKNHKKIKEEVFYQDGKLKSEGGFKDEKRNGVWRVWYQNGNIWSEGEFVDGKAEGF
                     RRVYYENGRLRYSGQYENDRMTGEWIFYDENGKKTDVKKY"
     gene            18114..18818
                     /gene="pyrH"
                     /locus_tag="PKY63_07830"
     CDS             18114..18818
                     /gene="pyrH"
                     /locus_tag="PKY63_07830"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPF00558.1"
                     /translation="MKRILLKLSGESLAGKQESGINAEMLASFADEIKSAVDKGAEVG
                     VVLGGGNIFRGLSGEKKGVSRVQGDYMGMLATVINSLALQDALKQREIKCEVLSGLEI
                     SPLCKKMSGPVAIELLKNKTVVIISGGTGNPYFTTDSAAALRAVEIDADALLKGTRVD
                     GIYTADPEKHPDAVKYDKLTFDEAYEKHLNIMDMTAFTLCKENNMPVIVFDIHGKGNL
                     EAILEGKKIGTIVKNK"
     gene            18833..19396
                     /gene="frr"
                     /locus_tag="PKY63_07835"
     CDS             18833..19396
                     /gene="frr"
                     /locus_tag="PKY63_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPF00559.1"
                     /translation="MSDESVLLLEEAKEGMTNAIAHLERELVKIRAGKASPSIFDSVK
                     IDFYGTMTPLAQVGNINIPDARTIVIQPWDRSVLGAIEKAIMAANLGFTPVNNGEIIR
                     ISIPPLTEERRKEMVKKVKVEGENAKVAVRNVRRNVVDSARKLIKDGVPEDEVKGLEK
                     DIQTATDKSIEKIDQIIAAKEKDIMTV"
     gene            complement(19724..20062)
                     /locus_tag="PKY63_07840"
     CDS             complement(19724..20062)
                     /locus_tag="PKY63_07840"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPF00560.1"
                     /translation="MKTILSLFVLLFAFTLVGKSQVVYSCSYKSDAQVKVYVADYKSD
                     ADLVVYKCSYKSDATGNDGLWFFAEYKSDADKTIYFCDYKSDADLVIFFADYKSDAGW
                     RNSSKKHLMY"
BASE COUNT         5923 a         3894 c         4156 g         6119 t
ORIGIN      
        1 cgattggtaa tgttttattt tgggcagaat ttcatcaata aaaacatgca cgcctttgtt
       61 ttgcagaccc actgcattca gcattcctgc agcggtttcg gccatgcgcg gataagcgtt
      121 gccttgccgt ggctcgagcg tagtgccttt tacaatgatg ccgccgagtt tattgatgtc
      181 cagaaaatcc tcgaattcgt agccgtagcc gaaggttccg ctggcagtga gcacaggatt
      241 ctgtagttca agattaccga tgtttacttt cagatcagcc atggcacacg ggattatggt
      301 tccagtcctt cagttgcgtt acgtcgaaaa cggggccttc catgcaggcg cgcacattgc
      361 cttcgtgtgt actgacggtg caacacagac acgatccaat gccgcatgcc atcaaatttt
      421 cgagcgacac ttcgcaatac acgccggcat cggttgcaag ctttccgatg gctttcatca
      481 tcgggtcggg cccgcagaca tatactttcg aaaatgattt aagatccgca ccaaaccatg
      541 ggtgtgctgt gatcatacct ttgtcgcctg ccgagccgtc ttcggtaaga atggctacct
      601 gagccaattc gctgtatgca tcggctgtaa gaatatcggt cgatgttttt ccgccgatgg
      661 ctacatggca gtcggctccg gtttccctga gctttcgtgc cagatacagc atgggcgcaa
      721 caccgcatcc accgccggcc agcagcactt tctgattgtt gcgtgcaagc gaaaagccgt
      781 gtccgagcgg atatataata tcgacctgat cgccttcgca cagatgcgaa agtttttctg
      841 ttcccgctcc gacaattttt acaagcagat cgagcgtgtg atttttttca tccacatcgt
      901 gcaccgagat tggtctgcgc agaaatgttt ccgggcttcc ttcaacgagg acctcgacaa
      961 attgtccggg ccatatttcc ggatgatctg cgggaagctg aagagtgagc acaaaatgtt
     1021 ttgagttcag ttttgtgttc ttcagcactg gcagcaatgc cttgcttttt ttattcattg
     1081 gcgggcgttt cggatgtttc gtccttggct tcctcagcgg gtttatcgtc gatgaggttg
     1141 tgctcgacca gaatgttgta ccacatgaaa agtttcttca ggtcgcttgg atacacgcgt
     1201 tcgcggtccc attcgggata cactttgtcc atgaatgcaa taatctcttt tttatcagct
     1261 tttgcatcga tggctttttc gccgttgaga tttttgaaaa tggctaccag aacgtctttc
     1321 agaggcatat cttcgccggt agtgtagatg ctgatgtcgg ccagcgcact tacgcgatca
     1381 gtggcaaata caggaatttt ggtgccgtcg agaagacttt caacgagcat tccacttttg
     1441 gtctgtgaga ccagagaaaa caatccgggc tttccggaca ctgcgatgat ttttgataaa
     1501 tccatattgg taagttttga tatcagatat ccgatttgag attttagatt tttgaaggga
     1561 tctttatttc atacttcttc cttcagatct aaaatcaaaa atctaatatc atatttcgta
     1621 aatcattcaa cggttccggc agcaacattc acttccggag cgattttttt nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcccatgc atttggccag aattacgctg
     1801 tggaggaaaa tggccggctg ggttacacgg gtctgacgca aatcttcttc ggttccgccg
     1861 aacatgatgt ccgtgatgtt gaaaccaaga atggtattgg cctgatcgaa catctccttt
     1921 gccattggga agcggtcata gaggtctttt cccattccgg gaaactgggc accctggccc
     1981 ggaaaaacga atgctttcat actggtgaat tttgatgcaa aaatactatt tttaatgtgt
     2041 tgatgggata atatgctaat gtgttgattt tctgatacga taattgggtt aggggtttag
     2101 gggtttaggg gttgatatga ttaggggttt agaggttgat atgtttaggg gtttagaggt
     2161 tgatggggta ttaattgtga gaatatttta tttttgtaaa ccaacaattt caatcgaatg
     2221 atgaaacctt gctgtgttct ggtattgttc attcttagtt atgtggtttg tgcacagtct
     2281 catcagttgg ggacatctgt aagcatgatt ccgctataca gtaaattatc ggaaagttcg
     2341 ggcgcaactg tcgaaaacgc tgtaagcccg tattttaccg cttcgattga ttattgtttt
     2401 actgataaaa acctggggtt gcatttgagc gtttttaagg ggtttgcctt gtattccgtt
     2461 gaatactctt tcacgaacaa atgggtttat tggacggggt cttctggctc cgagggtggg
     2521 cgcattgaac ataacacgat aatgtttaaa tcgaaaattc cgtcaaaagg catatttgcc
     2581 ggattaaatt acaaggtttt tcaaagtgat aactcccgcc atagtttgtt tctgtctgaa
     2641 tctgtcagac tcgaatctgc cagatataat tacgttataa aagacaggca atatacacaa
     2701 gtgatcaatt acgtatctga ttcgaccatg acgtatgttt catacgattc aaccaatatg
     2761 ttatattcgg ggagcagtta tttggatatg aatgatgagg tatctgttta tgtttgcccg
     2821 ggtattcagt atcagtataa aataagtgat aagttttcca ttaatgtcag tgcaatgatg
     2881 attctgggct taggcgacaa ccttgctaca ttcaagcagt gtacgatcat tcctgttggt
     2941 cagattgggt ttggttgccg ttatcatttt cttgtgaaga gaaaaaactg attagagaaa
     3001 agacattcct gattactcaa aacgggaatt accttttctg gtcatttgac gaaaaccggt
     3061 aattaattcc agcacaaaag tgtacaaaag gttttatttt ttcaatctgc agcagatgca
     3121 gagggtaatc agattgaaaa ttgaacaaca attcaatatc aagttcaaac ttattgttga
     3181 aagcatgatt gtattttaga ccagtcaata atgataaaaa aggtgcattg cctggttcca
     3241 gtgttataac gtctgcagca gatgaatatg tcgatgagct tgattcggta taagaaggat
     3301 ataaactgta tgaataatag tgttttgtta tatatacaga atctttaaat tctttgtaac
     3361 ccactatgga atttaattgt gctatacctg aaaacataaa acggttattt ttatcatcat
     3421 ctgcagacaa taacacatta aatccagcaa acagtcctgt aacgaaaaat gtactctgat
     3481 aaatatatgt actataggta tcgattgaat atgctcctcc gtgatttgca tcggcaaatt
     3541 catgatgaga attatatttc atatcgagcg gaatatattg aaattgcata cccgctgaga
     3601 gccctaaaaa attctccatg tagttgtagc gaaattttat tccatatgac atatatttct
     3661 gcggttcacc agaagattta tccggatacg aactctcccg gggtttgaat gacataaatg
     3721 cagaatatgg cgaaataccg atggaatgct gctgcgcgct gcaatcaatg atgaaaagaa
     3781 tgcaaatgaa aaaataaata attctcatta aagtagtatt catgcaaata taacttattc
     3841 ttattgaatt atgacaaaca caaagatctt tttttgttcg gattatgctt tttgcctgtt
     3901 tcgctgcctt cttccctgtc cgctgcgaag ctggcgttga aacatttgaa gtcaattgtt
     3961 gtttacaggt tgtcacttca aacactcaga atgacagcgg gcagctgtgc attgtgcgtt
     4021 aagccttgtg cgtggagttt atcacgccga aggcgggact ttgtgccgca tgccctatgc
     4081 cgcatgcctc agaactctct cccgcaatcc ctgcaataaa atctgggttt acggtttccg
     4141 aacggaaaga aaaaaagtat ggaaagaaac agcagcatgt attttccggc ccatttgcgg
     4201 ccaggaccgg atgccacgtt gagcgatttg caatgggggc aggcaatcac cttttcggat
     4261 tcagtttcat tcagtatttc aagagcagca gtctgatctt tttcatcaat tatcacctgc
     4321 acgcctgcat ccattacgcc cgtgtaatgc ggcatcaaag tggaaaattt ttcattagtc
     4381 agaaaacatt caatgccggc ctgtttcaat ttgcccatta tgatattggc ttcgaatgag
     4441 tcgctgcagg ttttgatgcg gatgactgac atgattcgat tttatttgag tattgtttta
     4501 acgaaagaat ctgaagatta ctgaaagcaa agtgaataat gataactgct ggccggggta
     4561 tatccataat gcttggccat ctccagatcg ctgcaaccat aaggttttag ttcttcctcg
     4621 ccatgattta tcagcaacaa tccgcggtaa aaataagctt cagcatattc attatcgatt
     4681 tgaagggcct ttgtgtattc ttcaagagcg ccttcccaat cttctttctg ctcaagcttg
     4741 attccgtagt aaacatgttc ttttgcctgt ggattgactg tatgctggca catagcagcg
     4801 atggaaaaag caaagaatgt cagaaaaacg ataattgctt tcatcttaca agtgtttttt
     4861 ttcaaatgta caatatttgt tttaccggac ttcagtattt cattattaaa cataaaaaag
     4921 taaaggttta ttgtgtaata acatattgaa attaaatgta tatttgcgac atgggggata
     4981 tatcagtgaa aacaattgtt atttatcgtt caaagactgg ctttacaaga aagtatgcgc
     5041 aatggattgc cgaagaactg caggctgatg tacgggagta caagcgggtt acacccgaag
     5101 aactgcattg ctacgatacc attatttttg gaggagggct ctatgtcgga ggcatcaatg
     5161 gcctctcatc tttcagagag tttttgcctt atctgcgcga ccgcaaaatc attgtgtttg
     5221 caacaggtgc cacacctgac cgcaacgaaa ccacgcagga actgcgtgaa cagaatttta
     5281 gcaaagagca gcaggaatac atcagttttt tttatctgcg cggcggattt gattattcga
     5341 aactcggatt aaaagacaaa tttttaatga gtttattaaa actgaaactg cgcatgacac
     5401 ctgaatcaaa gcggactccc gatgaaacag gcatgcttgc ggcctatcag aatccagctg
     5461 atttcacaaa gaaaaagtat atggaggaac tcctggaact ggcccggcaa aactgattaa
     5521 agtggccagt tgccagtagc cagtttatta gttctgtcct ttcacaaatt ttgcacgaaa
     5581 cgattttccg ttctttatca actcaaccat gtagaaaccc ggattcagtg aagaaatatc
     5641 tacgctgctg ttttctgttt tccctaaaag aacctgcttt ccaaatgaat cataaacaaa
     5701 ataaaatgca tctgatagat gtgagtcaaa atacaactca ttttctgcag gatttggata
     5761 aacaacaata tcatgaaaac attcagtatt ctcctccacc gaaagggaaa tgctctgctg
     5821 gtagtcgcat ggttcgtcgc ttcgttcaga aacaagtatt gaattaatat tgaagacatt
     5881 gactttattt acaaatttgc atttctgatg agttgaattt cgatatggag gcaaagaaat
     5941 agaaacatta ataaaatatg ggagattttc atcctcgtat atattcaggt agcgatcaca
     6001 atagaaaaaa ttgttcaaag caccaatgct ttcaatccag attattgata tagtatcgtt
     6061 gaaatctgag ttctgcagca gaatggcggg caataaacta tcggaaacac tgtttttaac
     6121 ggtaaagacc gcagttgcat aaaaacgcaa cgtgtcccaa tcaggtgtgg tttgggtaat
     6181 aatattgttt atgatttttg gataaactat gagatcaata ctgtcaccga cctgaagcga
     6241 aaagtcataa agaagaaatt cagtctgatt gagataccgc acaaataatc tgcgggaagc
     6301 cgaatcttca cgaatgtaat attggtcata catcccatat tgggggtaat atggaggatt
     6361 aaaatcgtat gaatgcctga actcctttac atttttatac gaaatgccat taataattgt
     6421 atcatttaaa atgaaagtat tgaaggtttc ataggaaaaa tagtcacaat ccactgccgt
     6481 cagcagagat gtgtctgatc cagattgaac tatcggatga taagtctgtg cttgtatctt
     6541 atttccgaaa aacaacgata taattattgc aatagataaa aagaaaagtg gtgacagatt
     6601 gattgctttc ataatatcct ccgttcttta tttattaacg acaattttct ttaattgttt
     6661 ggtggcattg ttggatacaa atccaatgaa atagacacca tcctcaatac ccgacaaatc
     6721 gaaatatagt tctgtgcacc ctgccggtaa aatttcggaa cgcagaacaa ctccttttga
     6781 atctgaaatt tcgcaagtgt tgtaatttag agattgctca tttagaacaa tcatgatata
     6841 gttaccagca gggtttggaa aaacctgaaa aagttcgtag ctttcagaga ctggatccga
     6901 aactgggaat attttacttt tcccctcaga gtttaaaacc agtggttcat tataggtcgt
     6961 atctcctgaa attctaagca tattcaacgc atacatatca gcaaatccat ccatatttcc
     7021 cgaatattgg ttaagcaaat acttttcgtt ttgcgttaag ctgaaaatat ttctattact
     7081 gttttctaaa ttccattgca atgttgaaac tgaattatac gcttcagaat ttggcagacc
     7141 agatatcatt gctgtactgt ctttaaaaag taagatagct tttatgtccc aatcgttgca
     7201 taactgcgtg aacatgatta atggttcatt tcctccacct gtgctgtccg ccatccagta
     7261 cctgatttgt tgattaatat attgcttcct tttatcactt agtatagcaa tctttgattg
     7321 cagattttca aattctgata ccatgtttaa acctgacatg ataccattaa gtaggtttga
     7381 tggcaatgga actgatcggt taattatggc atcctgcact aattcagatt taattccatg
     7441 aacattagaa ttaaggacat ctcttagcat atagggattt aatgcgttat cgtttgttgc
     7501 agcttcagct aaaacagagt cggataggta gggcgattta gacaatagat tggtcgtcaa
     7561 agataaggca tcgctattag ttgcagttga gacttcctga gaaagagcag ctgtatttcc
     7621 accgtcaaca agtatgttta aaatgtttgt taatgaatca atttctgaat catatctctg
     7681 cacaatagtt gatttttcta ttccaccaga accaccgagg tatgacggac aagccgctgc
     7741 ttttgtatat gtaaaatttg taacggaatt tgtcaaattt cggaaataat aaggaatcca
     7801 gtttccggtt gaacctgtat ggcgatagta aataatagga tagctggaaa acacattatc
     7861 aaaatcagaa tattgatttg atgggcctgc atgtgaaaag gtgtttcctg caggagcaga
     7921 aacacttgta ttagccccct gatacttaca aataccgcaa gggctgcatg acgcatattg
     7981 agtaacatag atatcttttg aacaattcga aaaatcattg catttgattt ttaatccgat
     8041 agtagcaagt attcctctgt ttttgtctaa agtctctatt ccatatttaa gattgctaaa
     8101 tgtattatta taaataatat tattcactgc cccagagttg ttaacaacaa taccaaaaga
     8161 atttacagag gttgtgccgg taaacacatt ttcttcacac tgccatccat tgcactgatc
     8221 aagataaata ccatatccga tatctcgtgt tatgttaaaa gaattccttg taattacagt
     8281 gccggttgcg cctgaaaaat atgctgaatg atgattgtcg gcaaaatgtg ctgaattaat
     8341 tgacgcaata tggttagtat ttgtgttgat ctgtttaacg ccgtaatcaa gatttgcaaa
     8401 aatgctgctt tgataattgt agcatggact tgttccactt atacacagat catcaaccat
     8461 aaacgaagca tcgtaagaca aaatgcctat tcctttatca ctagcatttg aaatatctgt
     8521 tcgtgtgtta cggaatgtgc atccaaaaat accaattccc tctacgtcaa taagactaag
     8581 aaaggaataa ggaatcccat cgttatcaat aagtatatct gttgtttcga aaatacaatt
     8641 tttaataacg cttaaattac tagtggtaac agttggattt gatggattaa aattatgata
     8701 tgtatatata gaaacgctat tgttacagtt cctaaaagtc gcattattaa tagaaataat
     8761 tcctcctgta taattccaat caaacgaacc atcggtgttt ttctttattg caataacggc
     8821 atcagttgca ttttcaacaa gggctccgtt tttaagttca accttcccct gcgctccggc
     8881 cgtcagctgg ctcgcaacgc tgtttcccca aacttcgatt ccgccccaca ttgcactttt
     8941 acacccattt ttaatgatcc ctccatccac taccaacttg ccaccgcgct caatgcaaat
     9001 cttcgtacct tccggcatag tcagtgtatt tgtcactgtc aacgttgccc catttttaat
     9061 aagtattggc ctatccatgc gagtgtaagt gctataatta gtatttgttg aaatttctaa
     9121 tagtgcggtt tcatcagagc atggcagagc gcatttccaa agcccccacc cgtaggtgcc
     9181 tgcatataaa acattatcca gatgattaat ttccacatca tcaacaagac aattgtaaag
     9241 accggtgccg aatctctgcc agtcggcagt tccgtttttc caatacactc ctatatcagt
     9301 tgcagcaaac agaatatcat caccactaat tccctcaaga atttcaagat cattaacagg
     9361 tagaaatggc aagccagaac taatatccgt ccatgaagaa ccaccgttag ttgtcacaaa
     9421 aactcggttt gcaggggtgg atggagaaga catccccccc caaagtaata taaacttttg
     9481 atgaatttgt tggatggatt gagatatggg taattgcata atacccaaaa ggatatccag
     9541 gtacgttttg agttatgtca gtccatgtgg ctccaccgtt tgtgctttta aggagtttat
     9601 tataaacatt ggcggagtag gtaggattac tgaatgaaac ccaaagtaca ttttcatcag
     9661 cgtcgaaaac gtcaaatcct tgaattctct ctgtgtttgc aatctggggc attgtcgttg
     9721 aaaaattact aatatttgac cagcttgaca aactattatt tgcccaatca gttgttttcc
     9781 atatatcatg gtaacacgaa tatacagtat tactctgaat tgcattccca acaattgggg
     9841 catcatttaa atggaattca ctcgttgtga gagcgatctt ataagtttgc cctaaagtat
     9901 gtgatttaag gacattaaca ttatttccta tcccatcacc acaggttctt aacatgatag
     9961 aatcctttga attaaaaacg ccatcatatg cgtctccaac tcttgtattt attaagctcc
    10021 cattgtcaaa tacaaaactc ccattatcct gtgctccgca taagactctt tcagcctttg
    10081 tttctgaatc agaaacaccg taaaattgcg aaatacataa tccatttcca ttaatattat
    10141 aggcgatgtc gttttcgatt tttgtcagtc ctccatcatt cccaaagaaa agcacatcat
    10201 cttcaaatcc tcctccatta gtactctggt aaaaataact gcatcttaca tctacatgca
    10261 tttgagaaaa aacattatta aacgtgtaat tgttgaagtt tgctcgtgtc acagttagcc
    10321 ctccaatata ctgaatattc gggtctgagt cagaaatgac atattcttgt ttccaatacc
    10381 caacacctcc aaaaggagaa ttttgatatc cagggctatt atagtagatg tattcagatt
    10441 gtacaaggga atttgcattg taaatcccga aaaataaatc ggatttattt tgtgcatcat
    10501 tatcagtttg tgtcgaaaac atgaaataaa agttttctaa gtcaatttgt gaaactgcaa
    10561 ctgaaattcg ttcagtcaaa agaggcctaa aatcaaagtc aacccaatcc ggattttgat
    10621 cctgaaagtt ctcgcaaaag aataactcgg cctgaaaatg ctccaaattg ccattatcat
    10681 cataggaata tccatcagaa gaaatcatta ctttgtcatc agtcccggga agaaactcta
    10741 tatctctcaa cagttttttc ttccatgtca gacaatgagt taaactataa ttttgtgttc
    10801 caaaaacgat ttgccaggag ttgcctttat cattacttct atatactttt atatactttg
    10861 tcatcaacca atgctgcaat ttgatttccg ttttcagggt taacaataat ttttgaacac
    10921 atgtgtatct ctgtcaaact caatgacaat atcacagtcc aagtattgcc accatctgta
    10981 ctcctgaaaa ggccagaaga ataagtcaac tcaggatatc ctgatttccc agttgcaatg
    11041 tacatttcat caggattatt tggatttgct gtgatatatt gaaccccagt aattaatttg
    11101 actccatggt aatattgaga aaatatattg acatttaacc aatttgcccc accatcagtt
    11161 gacttccata atccggctgt attacttcca atccatattt catttatatc atcaggatct
    11221 acccaaactg ctgataccaa accattgttc tgatttgtgg aaccagttcc aggctggcca
    11281 tttggaccaa caagttccca gtttaatgat ccctgagttc tgttttccgc aaaagtgtcc
    11341 ccattaatgt aacataatga atcccatttc tctcttgcaa ctgcatagtt tgataaaaat
    11401 ccaccgtcgc ggtaagacgg aaactgcacc ctgtttctcc agaataactc ccatctaata
    11461 tagtctttat acccacttcc cggaattgat gatatatact cgttctttcc aagaatattt
    11521 tctgcctcaa tcttaataga gtcgtagttc gcatttgctg catttgctga aaaagcccat
    11581 ccgttaaaaa ctttaattga atctgtttgc gaatggcaat taattgataa tgtcagaaac
    11641 aaaaatcctg taaaaatccg gtttaataat tttatcttca tgatttaaaa ttttacaaat
    11701 atatttcaat ttttctaaaa caagacaatc aacatgtaaa atctgcgaaa tttttcattt
    11761 tataaaatca acagggatga atttttctat ttctctcaat attcattcac ggacatctca
    11821 cttcagaact taccactggt ttctcaccac taaaaaaaca cctgcattcc aaacatgacc
    11881 tgaaactttg caatatcggg atgatcgaga tagctgagcc tccatacgcc atcgacccgc
    11941 aggaatttga aaatgttttc aatgccggca ccggtttcaa tatatggttt tcccaaagta
    12001 tgcatgcctt ccggaaactc gctgtagttg cggttcgcag cactcaggtt tccgatgagt
    12061 cctttgcccc agaccacttc gcgccatttg agttttcgca gcagcggaat gcggttgagg
    12121 aaaaatccgt cgaagcggtg tgtgtaatac aggctcaccc attcatcgct gacaaactcg
    12181 aaataattca tcgtgttgaa tgaagtttca tcaaagaaat aggtttcatt tccttcgtgc
    12241 atcttcagaa acgggtaggg gagccgcccc cagatttttc ctgcttcgat cacatatttt
    12301 gaccagccaa tgtttcctac attgaaccaa tcgctgaaat aaaaggaggc tttctggtaa
    12361 ttgtagtctc cgccccataa atccttgatt cctttgctgt atttgaactg aatgatcgga
    12421 tatattgtac cgagacttgt gcggataaaa tcgctataca cataacgttc cttgtatgca
    12481 aaccgcattt cgagcgtgat ttcgctggtt gtgatttttt cgcgtgtggt gccttcgcca
    12541 aaattgaaca cagtatctcc gacaggaaaa atatctttat gcagaaaacg aacggtgttt
    12601 gaaaagccgg taaaccactc gtgttcgtaa tgggctttga gttcattgac aagagtaagc
    12661 ttatttgccg gattgcgtgt gatgaaactg gccagcagaa agtcttcgcg aaaccctgtc
    12721 ggactcatgc ccagctgctc catatcgtgt ttgaacaata tctgaaaacc gcggcgtggc
    12781 ttcttgttca tgagatacag aaaacctccg ccgaatttga atttctggtc ttttgttccc
    12841 catgcaacat aagccgaagg cattattttt ttgctgaatt tttccgatgt gcggccaccc
    12901 agacggaagc gtgcaccttc tatttcattg aagctgagca tggatgcata tggtccgata
    12961 tcaaatttcc cccaatttaa atatcctgat acaaacatgt caataatgtc gtacacggta
    13021 ttaaatgttg gcattttttt tatgctgtca accataaaat aaatggtctg ctcatcttcc
    13081 gacagcgtgt cgtgacgttc cttctgccag aattcaagtc ctttttcata ggcgtcatcg
    13141 gcaacaacaa cgcgttccgg agcattgtaa aatgaattat cttttgtttg tgtcagcgaa
    13201 tagttttata tgaggttgtt ttatgtccat aaaaacccat tgtatttttc tggtcatttt
    13261 gcaatacatt gaagtcgatg acaagacttt ctttctcggg catccaaaat cccgaatcgc
    13321 tttgctgaaa tgattgttca ataatcaggt catttacaaa attgatgttg gcatcattca
    13381 ctacgcgcat gctgaattgc tttacagccc agctggaatc ggccacccag aactcgcccg
    13441 aaaaagtcaa ttcctgtttg cgccgtggtt tgaacaatat tttgtagcat catgtgtcat
    13501 cgataatagt gctgtccatg aggtaatatt tataggacgt gagcgcaata tcattgatgg
    13561 ggctcatgaa atttttatca aacagcgtaa tgaacccatc ataaatgttt atattctgat
    13621 acatgttgcc cagatactgg gcaatgcttt cgttttcgac accggaaacc tgtacggcac
    13681 tgatgtattc gcgttttgat tttggattgc cgcgaaaata gattttcgat atggattcgg
    13741 taagaaacag gggcagatat acctttccgt tcaccagcga ggtgtcggta tattcaaata
    13801 tccatccgaa tttcttcagt gcacgttgac ttttgagttc atctgaaata ttattggcgt
    13861 caatttgtac tttgttgtaa acttcgactt cgtaaaatgc tgcgttgtcg ggagaattgt
    13921 tttttttgtt gcggattatt ttttttaaca gtacttcggc tggattttcg cccgggacga
    13981 taataacttc gtttaaagtg atattatttt gcgataaagc aatgcttaat tcctgaaact
    14041 gattttcttt aacgggcagg gtttgttgtg tgtaaccgag gcaagaaaca atgatggagt
    14101 caccagacgt gctactttca attttgaaag atccttcaaa atcggtggtt gcaccgatac
    14161 tggttccctt aagggcaata ctgcaaaacg ggattggatc tcctgttgct gaatctgtca
    14221 catgaccgag aatgcggata ttttgtgcag caagattgtt gcacagaaga gcaaaaacga
    14281 taataatata tgtttttttt agagagcatc gggtttattc agctcattcc agaattctac
    14341 agcacgcctg gcatgaggga ttacaatcgt tccgcctacg agaatggcaa ttccgaatat
    14401 ttcatgaatt tctgcatcgg aggtgccgat ttcgcataat ttttcgagat gatattttac
    14461 acaatcgtcg catcgcagaa cgagggacga aacaagtccc agcatttctt tggttttggt
    14521 attcagtgcg cccggatcat aggccatggt atcgaccgaa aacaagcgtt tgataacttt
    14581 attgtcgcct tccagaattg ctttgttcat tttgaggcgg tattccttaa attcctttac
    14641 ttttgacatg gcatttgttt gattaacaaa aatagctatt ttgttaatgg agcaaatatt
    14701 tatttttgga ttgcataaat atttttatct ttactatgaa atattacttt tgcagttcga
    14761 aaacctgagg gtggattctg aataatgaaa caaattgaga ccgacgatat aaaaggtgta
    14821 ctgaaaacgg tgtttggctt tgataaattc aaaggcgagc aggaagagat tattgaaagt
    14881 ctccttgagg gcactgatac ctttgttata atgccaacag gtggtggtaa atcattgtgc
    14941 tatcaattac cggctctggt gttgcctggc actgccattg ttatttcgcc gttgattgcc
    15001 ctgatgaaga atcaggttga caacattcgt aattttggta cggaaaacgg cattgcccat
    15061 gtaatgaatt cttcgcttaa caaagctgaa atggcattgg ttcacgaaga tcttttatcc
    15121 gggaaaacaa agttgttata cgttgcacct gaatctctga ccaaggaaga aaatgttgaa
    15181 tttcttcagg gtattgatat atctttttat gctgttgatg aagctcactg catttcagag
    15241 tggggacatg atttccgtcc ggaataccgg cgtataagac caatagtgga agccatcggt
    15301 aaaaatgtgc caatcatagc cctcacggcg actgcaacac ctaaggtgca acaggatatt
    15361 ctcaaaacgc tgaagctgaa agatccaaag gtttttatat cttctttcaa ccggcccaat
    15421 ctctattacg aagtcagacc aaaatataag gatgttaaca aagaacttct gaaatttgtt
    15481 aaagccaatg aaggaaagag cgggattgtg tattgtttga gtcgtaaaaa aacagaagag
    15541 ctcgctgaat ttcttcaggt gaacggaata aaggccttgc cttatcatgc cggacttgag
    15601 gccgctgtgc gaaggcaaaa tcaggatatg tttctgatgg aagaagtgga tgtgatgatt
    15661 gcaaccattg ctttcggaat gggtatcgat aagcccgata tacgctatgt ggtgcattat
    15721 gatatgccca aaagtcttga aagttattat caggaaaccg gtcgttcagg tcgtgatggt
    15781 ggcgaaggac tttgcattgc tttttatagc attgatgatt tgcagaaatt tgaaaaattt
    15841 tcgaaagaca aaggtgttgc agaacaggaa atagcacaac atttattgct cgaaaccata
    15901 tcgtttgccg aaagttcggt ttgcaggcgt aaacaattgt tgcattattt tggtgaaaat
    15961 tatttgcatg acaattgtca tagttgcgac aattgtttat tcccgcgtga tacctttgaa
    16021 gcggaagatg atgtttgtct ggtgctcgat accattgaag aaacgcagga acaatttaaa
    16081 tcgaaaagta tcgttgatat cattcttggc agtaagtctc cttcgatgaa acaactgaaa
    16141 gctgagaaac tggaggtgtt tggaaaaggt ggcgaaaagt cggagagatt ctggaattct
    16201 gtcatcagac aaatgctgat tatgaacttc atggcaaaag aaattgaaaa ctatggagtt
    16261 ttgaaaataa cggaaaaagg aaaagaattt ttgaacaacc cacattcaat tatgttgacg
    16321 aaagatcgta acatcgaaga agaaaaagaa gaagacggcc ccagttctgg tgatggccgc
    16381 agcagtggaa ccgacaaggc gctttttgcc atgctaaagg atctgcgtaa acagattgcg
    16441 aaaaaagaaa atctgccgcc ctttgttatt tttcaggatc cctcgctcga agatatggcc
    16501 attcagtatc cgataacctt ggatgagttg aaaaacatca cgggcgttgg tgcagggaaa
    16561 gccggaaaat acggtgagcc attccttaaa cttattgccg gttatgttga agaaaatgaa
    16621 atcatcagac cgatggacat ggtggtcaaa tccactgtga acaaaagcaa gctgaaggtt
    16681 tttattattc aggccattga ccgtaaagtc gcgctcgagg atcttgctgt ttcgaaaaat
    16741 cttacttttg aagaattgct cgacgagctg gaacgcattg tctcaagcgg tacccgcatt
    16801 gacctgaatt attatatcga tgaaaatatc gatccctatc atcaggatga aatttttgaa
    16861 tatttcagaa cagctgaaac cgattcaatt gatgatgctt tgacagaact tggtgaaaat
    16921 gagtacaccg ttgaggaaat tcgtctcatg cgtattaaat tcatgtcgga aatcggcaat
    16981 taattttggt atatggaaat gaacctaaac aatcaggaaa atagctttgt gcctgatgaa
    17041 aaaaagaaaa gctgtcttcg cagtccgcag atgattttta atatcgcagc gggagtactt
    17101 attctcattc ttctgatatt gcagttttac aatccgtttt caaaagaaaa tggcattgtt
    17161 gtgccagatg gcggacttaa tattgcgtat gttgaatctg acagtatcat gacccaatat
    17221 gatcttgttg atacactcaa gggtgacctg gtgaaattga atgatagtct ggataaggat
    17281 ttcaccaaac gtcaggaagg ttttcaggca cgtgtcaact tgtttcagca gaatgttcag
    17341 aatggaaaaa ttcagaccaa agatgattat agtcgtcagg aatcggcttt ggctgctgaa
    17401 caggaacaat tgatgaactt gcgcgactcg tatctcgcac aggttcagca aaaacagatt
    17461 gctatgaaca atcagattct ggattcgatt gtgagtatta taaaacaata tcctgaggaa
    17521 tttccttatg actatgtgct cgattacaac agtggcacag gaattctgta tgccaatgac
    17581 aaattgaata ttactgtcaa ggttgttgat cggttgaata aagaatacaa caagtaatgc
    17641 gtagtctggg tttttttctg tttttttctg tcatcgtgtt tgcattgatt tcgtgcggtg
    17701 gtgacaaaag tgtccgagtg gttgaaaaaa cctatccaga tggtaaagac aatattgtag
    17761 tgtattattc gaatgataag aatcacaaaa aaataaagga agaggttttt tatcaggatg
    17821 gcaaacttaa atctgagggc ggctttaagg atgaaaagcg caatggggtt tggcgagtct
    17881 ggtatcagaa tggcaatatc tggagtgagg gtgagtttgt tgatggcaaa gccgaaggat
    17941 ttcgtcgcgt gtattacgaa aacggccgac tgcgttattc aggtcaatat gaaaatgatc
    18001 ggatgaccgg tgagtggatt ttttacgacg agaatggaaa gaaaaccgat gtcaaaaaat
    18061 attagtttta cctggaggtt tgaatacctc ctttttttta aatttgcagc atcatgaaaa
    18121 gaattttgct taaactcagt ggggaatcgc ttgccggaaa acaagaatca ggtattaatg
    18181 cggagatgct tgcatctttt gctgatgaga ttaaatcggc tgttgataaa ggggcagaag
    18241 ttggtgttgt gctgggtggt ggaaatattt ttcgtggatt gagcggcgaa aagaagggtg
    18301 tgtcgcgtgt gcagggcgat tatatgggaa tgctagccac ggtaatcaat agtcttgctt
    18361 tgcaggatgc attgaaacaa cgtgaaataa aatgcgaagt gctttcagga cttgaaatat
    18421 cgcccctctg taaaaaaatg agcggccctg ttgctattga attattgaaa aataaaacag
    18481 tggttatcat ttcgggcggc actggcaatc cctattttac tacagattct gcagctgcat
    18541 tgcgtgctgt tgaaattgat gccgatgctt tgctgaaagg cacacgtgtg gatggcatat
    18601 atacagccga tcccgagaaa catcccgacg cagtgaagta tgacaaactc acatttgatg
    18661 aggcatacga gaaacatctg aacattatgg acatgactgc tttcacgttg tgcaaagaaa
    18721 acaacatgcc ggtcatcgtt tttgatattc atggcaaagg caatctggaa gccattcttg
    18781 aaggaaagaa aattggaaca attgtaaaga ataaataaga agtaaaacag atatgagcga
    18841 cgaatccgta ttattacttg aggaagcaaa ggaaggaatg accaatgcaa ttgcgcatct
    18901 ggaacgcgaa ctagtgaaaa ttcgtgccgg aaaagcgagc ccttccattt ttgattcagt
    18961 caaaattgac ttttatggaa ccatgactcc gcttgcacag gtagggaata tcaacattcc
    19021 ggatgcaaga accattgtga ttcagccatg ggaccgcagc gtgctgggtg ccatcgaaaa
    19081 ggcaattatg gctgccaatc tgggttttac gccggtgaat aatggagaga ttattcgcat
    19141 ttcaattccg ccgctcaccg aagagcgacg taaagaaatg gtcaaaaaag taaaagtaga
    19201 aggcgaaaat gccaaagtag ctgtgcgcaa tgtccgccgc aacgtggttg atagcgcccg
    19261 taaactcatc aaagatggtg ttcccgaaga tgaagtcaaa ggtcttgaaa aagacattca
    19321 gaccgcaaca gacaagagca ttgaaaagat cgaccagatc attgcggcca aggaaaaaga
    19381 tattatgacc gtgtaggcac aacgttcaat gcacaaagcg caacgcggag tgcattattc
    19441 aaaatatttt attgataata ccggctactt gcttgtcccc ggagcccttg cgggcatgcg
    19501 ggctaccaat attttgtccc gctgggacag tctggtactg cgtagctttt tttacgcctt
    19561 cggctcaata aatttatcag agatcccagc acgaaattga gagctgatca ctgataatcc
    19621 cgtgccttgc agacgggact tcgctgacga ctgaaagctg cccggccttg attcactgct
    19681 cactgctcac tattcaccgc tattcggccg gcttttctgc gtgctagtac atcaagtgtt
    19741 tcttgctgct gttgcgccag ccggcgtcgc ttttgtagtc agcgaaaaat attacaaggt
    19801 cagcatcgct tttgtaatcg cagaaataaa tggttttatc agcatcactt ttgtattctg
    19861 caaaaaacca taatccgtcg tttccggttg catcgctttt gtagctgcat ttgtaaacaa
    19921 ccagatctgc atcgctttta taatcggcca catatacctt tacctgcgca tcgcttttgt
    19981 atgagcagga atacacgacc tgacttttgc ccaccaatgt gaatgcaaag agaagaacaa
    20041 agagagacag aattgttttc atgttttttt gtttttatca aagatagaaa aatttttggc
    20101 gcggggcaat aacaaaaaca atgccttagg gtgactttat atagggcctg ctgaaaaagt
    20161 ctgaggtgcg ccatattcga ggcgacaaga cg
//
LOCUS       DAOPKE010000051        19922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2868_length_19922_cov_12.497408, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000051 DAOPKE010000000
VERSION     DAOPKE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_19922_cov_12.497408"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..585
                     /locus_tag="PKY63_07845"
     CDS             169..585
                     /locus_tag="PKY63_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00561.1"
                     /translation="MKYFVALIFLISAFFSSCEKDEERIPYVPVNIYIDINNANYNDL
                     NAIGGYIYLTGGYKGLIVYRASYESFVAIERACPYHPNSDCGRLVVDTSGLTISDPVC
                     GSRFLITDGSIVNGPATRPALTYSTLYDGFYVRVYN"
     gene            661..1104
                     /gene="tnpA"
                     /locus_tag="PKY63_07850"
     CDS             661..1104
                     /gene="tnpA"
                     /locus_tag="PKY63_07850"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPF00562.1"
                     /translation="MPHSYNRIWIHATWSTKDRRKIIHSSFEREMYRYMYNQFKQCGC
                     AVKIINGVEDHVHCLFLLNPSKSVADVIKQVKGSTSHFINAGDFDIKDFAWQNGYGSF
                     SASESVTDYIASYIENQKHHHKRKTYDTEFTGFLAENHIDPMDNE"
     gene            1199..2347
                     /locus_tag="PKY63_07855"
     CDS             1199..2347
                     /locus_tag="PKY63_07855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF00563.1"
                     /translation="MLYDFNFNQHPLWMIVVGGALVLSLLVFLFYYLFFFRRFAFYKT
                     KHHQTDEKKLPPVSIIIAAKNEFLNIRENLPFLLNQDYPEFEIVIVDDHSQDDTWDLL
                     CAFKIHNPNLRTVRLTDSVVVCEGKKFPLSVGIKEANYPHLLLTDADCRPTSPFWIQK
                     MIEQYNNGTEIVVGYGKYYRHKTLLNLLIRFDTMRIALQYFSAALSHFPYMGVGRNLS
                     YKKELFINSNGFTNHYDLSSGDDDLFIAEHAEKNNTAICIESDAYTWSEPKHSFASWW
                     HQKRRHLTTGNRYRFKHKLYLGLYQLNLLLFPALAALLLAFQYQILAVASVVLIKWST
                     QWIIFSGTTKKLKEKKLLLISPVFETIMAVLNALLYVSNIIRKPGTWK"
     gene            2338..2943
                     /locus_tag="PKY63_07860"
     CDS             2338..2943
                     /locus_tag="PKY63_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF00564.1"
                     /translation="MEVSPNFTEKAKRDYELVQRALDHGDQKAYTELMKNYREAIYFM
                     MLKMTNDATDADDLTIEAFGKAFRNLPQYTPEFAFSTWLFKIASNNCIDYIRKKRLVV
                     TSIDQGVHNEDGGMAQNIPCEHPDPEEAIIKKQKHKALREVVEKLKPHYRRLVELRYY
                     KEYSYEEIAEELNLPLGTVKAQLFRARDLLCQILSKDQDSI"
     gene            2943..3584
                     /locus_tag="PKY63_07865"
     CDS             2943..3584
                     /locus_tag="PKY63_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF00565.1"
                     /translation="MAIQLHTFVFNGFGVNNYILEAENGDCIIVDAACSNDSEKEQLK
                     SFFSDSRRKPVMLINTHTHIDHVLGCAFVQKKFGIPWLMHSEALPTLNEAPVFASLFG
                     INNMEMSKPDNFLEDGQELVFDNNTIKILYTPGHAPGSICLYIPAERMVITGDVLFNG
                     SIGRTDLTGGNFDVLMESIHTKLLTLPDDTIVYPGHGPATTIGDERTGNPYLG"
     gene            3801..5474
                     /gene="recN"
                     /locus_tag="PKY63_07870"
     CDS             3801..5474
                     /gene="recN"
                     /locus_tag="PKY63_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPF00566.1"
                     /translation="MFLISAMLQSLHIQNYALIRQLDVDFASGFTVITGETGAGKSIL
                     LGAIGLLIGNRADHGVLLNEQQKCIIEGQFALEKNAFENFFSDNEIDFEGELIIRREI
                     SPGGKSRAFVNDTPVQLSMLKDLGDQIMNIHSQHETLLIAGNRFQMQTLDSYCGNNAL
                     LAEYGEYFKKYSDCQHRINELKEQIARRLSDADYFGFLFKELVQASLSAEEFASLEQE
                     QIMLMNAEKIGEGLFASLQILSESDDNVQITLRRVEQYLKNASQYLPELTELSERINQ
                     SLIEISDIADTLNRMSDKAEINPARLEKVNERVDEYNRLMHKHKVSDIEQLLAIKDEI
                     EAKLEDNSNLELKLESEEKMLTELDKELKKISADLDKKRRKAAPAFRDAILEILNNLG
                     MPNALFEAALENTDSYKPDGTNSIRFMFTANKGEKLQELGAVVSGGEMSRVMLAIKSM
                     TAQRNMLPTIIFDEIDSGVSGLIAAKMGNILKKLSENHQVIAITHLPQIAARGEDHIH
                     VEKNDAGEKMQTILCKLDSDARVEVIARMISDEKMSDSALEMARQLLSN"
     gene            5496..6113
                     /locus_tag="PKY63_07875"
     CDS             5496..6113
                     /locus_tag="PKY63_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00567.1"
                     /translation="MPSKKYFLDTDKKEMLDLSWKAGFHTVTVSYNGILLSTMNREEA
                     STGKAVELPDGRIVDIKLESGFFAALTAKINGRHIPGTQGDPKYQLKQVFYLMIILGI
                     LNIIIGLIFAVTDVHIDGLESIGYINVAIGLVYIALGYAVMQGSMIALVLITLILFGD
                     LILAAMYSAQSGMTAGIIMKVFFVIFVVRGFKYMKEFRAERHVQS"
     gene            complement(6212..7822)
                     /locus_tag="PKY63_07880"
     CDS             complement(6212..7822)
                     /locus_tag="PKY63_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPF00568.1"
                     /translation="MKTVLFFTMLLSAMFVLADDEKVLSTSIRNVTVFLSGAQVERTG
                     TIYLNTGNYDIVIQDLPTTIDPNTIQVSGKGNITVLSVENRINYLKSQIKSKKVLVLE
                     DSLEMLRGQINYQNAMLTVYNNEEAMILANKEIGGDDSGVDIAALKANADFYRIRLTD
                     IKTKQLEVGIKLARLNKDIARVQQQLNEYNAKQNQPSGEIIVRVIANAAGNATFVTRY
                     NVQNAGWAPMYDIRATDFGNPVKLTFKAGIYQSTGENWEKVKLTLSTANPNQSNNKPV
                     LNPWYLYFNNYSYRSQSGNNRAAVPMSVADSKAEVSKEEYDEDKNFESAVAYTSVTEY
                     PINFEYAIDLPYSVETNGKARIVQVKEYELAALYQYYAAPKLDNDAFLLARVTGWEKY
                     NLLPGESNVFFQETYVGKSYVNPLTALDTLEVSLGRDKSISIKREMVKDYTSDKFIGT
                     SRKVTKGWEITVRNNKSKEIDIVIDDQFPITTNKEIEIEKIEFSGGIVDENTGKVSWN
                     LKLKPGETKKLTIKYSVKFPKEQNIIVY"
     gene            complement(7927..10527)
                     /locus_tag="PKY63_07885"
     CDS             complement(7927..10527)
                     /locus_tag="PKY63_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00569.1"
                     /translation="MYWGGQFGAEIDLDTWFHIGSDFIISGFTTGAVDVEYPVTVEME
                     IPNDDTFCPGEEVTIESSYDVRPGWDLTTHFPTAGVIGLYLNFGFGIDLDATICVFGC
                     TTFPVIDVNVPFDTIPIIELNSQTGVFTYPCFVPTAFPPIQICHDTILPITFNNLFGI
                     GLSGSITLPYVETTDWLDVIDECNQNLYAAGDCTWVSLELDVIQFLSAIAGLIPPPTG
                     PAVQQFLGMLSGTYDLGAGFTIDYMLFSAWFTILSTMQQDFAFEPDVWNNLVLSVPVE
                     YTVTDPSSGNMVVDSGFSDNISFKACNNLNYIWPCHNVNQIDFDLSHSLTNDFTNHTW
                     DSLSFDFSIAALEFTINIPSFPIVPPADIPDLCIDVPFSDTISGNEYLKQACMPGVTI
                     PEVRAFESKLSIHIGPLFSYTWPLGYIPITWYEDTWELAGFNDTVFPGFSMATSCPGL
                     EIVSFDAEDVLCFGDSTGTLTIVVQNGATPYTYIWSTGDSTTTVSTSNSVSGLESGWY
                     YVTVFDANGCSVSDSAEIIDAYPPIEVIVSKVDVTCEGGSDGSVSLVVSGGAVPYSYT
                     WSPPQVNAPSVSNLPAGTYTITITDAVGCDTTVSTTLIELHPLPEINIVASPTEGCQP
                     LFVQFYETTPDSGQTYYWDLDEGHHFSSDKNPSLIFEQDGVFDVFIAVTSIYGCVDSL
                     RVNNMINVYRKPVAEFRPYPSAPTLADNTVLFFNESTSLYQSEWTFGDGGFSMDTYPT
                     HQYNDTGLYVVELIITTEHGCKDTASDVIYVKDVVTMYVPNSFSPDGDGINEVFKPIG
                     HGIDENFYTFMVFDRFGKMLFITTDYEQGWDGTFNGKRCALGSYTYEIFYRDTEGIKR
                     KLFGGIELIR"
     gene            complement(10499..10735)
                     /locus_tag="PKY63_07890"
     CDS             complement(10499..10735)
                     /locus_tag="PKY63_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00570.1"
                     /translation="MKKLLFTVLVSLFVLQNAKAQSYVISNDIDWATYNQNMWGPNGN
                     PWNINMNINLFDVQYDTAVTIGDIVNVLGGAVRS"
     gene            complement(10741..11274)
                     /locus_tag="PKY63_07895"
     CDS             complement(10741..11274)
                     /locus_tag="PKY63_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00571.1"
                     /translation="MKFFLMLFFSGVFVVPSMAQQDNSPEMLIDTTYHKVNERFYFDS
                     TGVSIIPPAYFMPFIQKDKTGFIHKGAGSTIQVQILDGMIYSMIAGGLTEEEFLRQNA
                     TLKEHYTLLTNDDKKAEFFIVSFFIKALDKDVEYERMMLLTGDHKRTVWVSANYPVMA
                     KPVVFSVLKECLLTVEF"
     gene            11408..12409
                     /locus_tag="PKY63_07900"
     CDS             11408..12409
                     /locus_tag="PKY63_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00572.1"
                     /translation="MKKVAISIIVFFVALQGFCQNDSIKPDPGQFFADIHTFYIYDVN
                     GQTNPRSAFGINTAIMGYKKSLSKKIQATILYDVTRTTNFIFPDTIGISSYFEGSKYT
                     AFLKMAEIGWQPKPWIELYFGQLLNEQYLTIQDKHWGLRYVNTTAQEYFRFGNPADFG
                     ARIKLRPSEKLIMSVSIMNGEGPFRYQDNESLFQYCINAEYRPQEHLIFKIYGDYQPH
                     ASGLNKDRCAISVFAGYKKGKWMAGAEYNRVENNIFLNNSDLSGMSIYSSFKTGKKTS
                     VFARADHMFQYGDVENEAYFMFGAEYRPAPAFGLTLSVKRNTWLNTLTPGIQAGIRF"
     gene            12548..17596
                     /locus_tag="PKY63_07905"
     CDS             12548..17596
                     /locus_tag="PKY63_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPF00573.1"
                     /translation="MAAILFVFAASAQTVRIPIQGETTKMKIESNNGLSFTAVLNISE
                     FTLTKVETPAGYFTVVDLPQFTKRFDDGRPAIPLFSKLIETPTRGPASISNISYDIAE
                     FDLDDLGFSDKVIPAQPSWSKNTDPSEIIFHYDQAFYGINSFSNNQLIRIVEGGEMRG
                     VNMGTVVIDPISYNPVSNKIEIKYNIRFTVNFDPIGYAQYNQRKANFFSPAHDGFYKE
                     LANYIEPATKDIITTYPIKFVIVADRMFETALQPFIQWKTEKGFTVIEAYTDQSAVGT
                     TTTSIKSYLQGLYDAGTPTDPSPTYVLFVGDVAQIPAFNGASMNSHVTDLYYACYGGS
                     TDNIPDIYYGRFSAQTVAQLTPQINKTLMYEKFTMPSANYMDTVLMIAGVDGTYAPTY
                     GNGQISYGTNNYFNAAHGIYSHTYLYPASDGTSVPSEIINIIGDGVGYANYTAHCSTT
                     GWGDPSFTTTGIASLNNANKYGLIVGNCCQSVKFEDSNCFGEAILRAENEGAVGYIGA
                     SDYSYWDGDYWWGIGNTSSITSNPTYAGSGLGAYDKAFHDNGEATSEWFISNGQMVVG
                     GNIAVQASTYSTTFKKYYWEEYHLMGDPSVMNYFSKPDPLTISYDNPAQVGNTSLVVN
                     TEAYTYVAISLNGVLLDAQYTGTNTSVTLTFPAFTSVDTALVVATKQNKIPHIGDLPI
                     EDVSVTLDAQVLSILKPTGSYACTNISETPRIVIKNRGINNLTSTTIKYKLNNGTLQT
                     ASWTGNLATNESDTFDLTTITIPAGTNTFLAYTINPNGGTDMNIANDSASITFVAEDL
                     PIVAAFTASPTAFCSAPANITLDNTSTNASSYFWDFGDGTTSTLAEPTHTYTSVGLYT
                     VSLTANGGVCGSDSYDITVMVGSQPPVINDTTACGAQSFVLNAGGSNINWYSDATGTT
                     QIGSGNTYTTPVITNTQTFYLNSASSSQYTGGKPDNSGTGGYFTNTNLHGIVFNCTSP
                     VTLKTVVLYSDDANSKTITLTDASDNVIDQATVSVISGENTVTLNIDIPVGNGLKLMG
                     PASPNLYRNGSTTGPNLYPYSVGPYIELIGSTASGYETQYYYYFYNWVVEENCTSAME
                     SVTCTILPVPVADFSATTTNTCENTTITLTDATSGSPNAWIWSITPTTYSFVGGTSAA
                     SQNPQIQFTAPGTYNVQLTATNNCGSDIESKNNLITVATPPAQPSAIAGNATPCANTT
                     GLTYNVTNVGGVSYNWTVPTGWSITSGNGTNTITVDAGVNAGTITVTPNNGCEGTPQA
                     LAVAINTAPDQPSAINGQIGPCQAGSETYFVTNVPGVTYTWSVPADWIINSGQNTSSI
                     TTTIGTQSGNITVTPSNACGNGTAQILNVTSSLMPNQPSAISGDNTPCQTSQTYTVSA
                     ESGITYTWSFPTGWNILSGQGSNSITVIPSNTAGVISVIPSNGCGAGTAQTMNATPEE
                     MAEQPSAIAGTTTPCSFTTQTYSVTNVTGITYTWTLPSGWAVNTGQGTNSITVTTGNT
                     NGTITVTPSNACGSGTPQTLDVSSAAYPLVDLGADTSICEDQILILDAGNSGSNYLWS
                     NGATSQTITIDASLLTPGNGYNYQVTVTNSSGCASTDAISVNILICSGIDDNFETVAA
                     IFPNPAQDNITVTCANSIQRIEILDTKGAVVYNQNFSGNVLNINVAALSEGLYYIRVT
                     TASGSDVLPLTIDK"
     gene            18414..19106
                     /locus_tag="PKY63_07910"
     CDS             18414..19106
                     /locus_tag="PKY63_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00574.1"
                     /translation="MKKLFQLALIMLFPFWVNSQDCPEYFPLKTGNSWELTNYNKKDK
                     IIGSNSYAVKSVTESATGYEAIVTTSAINEKGEVEGSGDLIMKCESGVFYLDMKNFLD
                     QSAMGNNPDMTVTMTANDLQYPSTMTEGSNLPDASITYQMSSGGMVVMTMTVNITERK
                     VIGKESITTPAGTFEVWKLTARYQSKTGFITTKLSTIEYISIGGGIIKSESFNDKGDL
                     MGYSILTKLSKL"
     gene            complement(19327..>19922)
                     /locus_tag="PKY63_07915"
     CDS             complement(19327..>19922)
                     /locus_tag="PKY63_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPF00575.1"
                     /translation="YSTANSMKYAKSFFGIIDLLSILPTYLGLIFTGAHSLMIIRLVR
                     VLRIFRIFKLTKQLTEGRVLLTAFRASSSKIMIFLMSVLLIVCVFGTVMYLIEGPVNA
                     SFDSIPRSIYWCIVTVTTVGYGDISPVTSTGQFLASILMICGYAILAVPTGIMTGEII
                     RSEKAATAQTCPYCTRQGHDKDAVYCKYCGKPLHERV"
BASE COUNT         6267 a         4535 c         3884 g         5236 t
ORIGIN      
        1 caacattagc actatgtatt tcagaattct catgtgtgaa aaaatgctga tgaaagaact
       61 tcaaagggcg aaaatacaac ttttttttct ttttgacctg aaatgggaaa gatttatgaa
      121 aaagtttttc actatctttg gtgtataaat aatgaacgga atcggaacat gaaatatttt
      181 gttgctttga tatttttgat ttcggctttt ttttcatcct gtgaaaaaga tgaagaaagg
      241 attccgtatg tacccgtcaa tatttacatt gacatcaaca acgccaatta caacgatctg
      301 aatgccatcg ggggttatat ttatctcacc ggcggctata aggggctgat tgtgtaccgc
      361 gcttcgtatg aatcatttgt agctattgaa agagcttgtc cttaccatcc taattcagac
      421 tgtggacggc ttgtggttga cacttccggg ctcaccattt ccgatcctgt gtgcggctca
      481 aggtttctga tcaccgacgg aagcattgtt aatggtccgg ccaccagacc ggccctgaca
      541 tacagcacct tgtacgatgg attctacgtc agggtatata attaattttt cataaccatg
      601 ggattaatcc catggttatg aaaactcaaa tgctgggaaa acaacaacaa ttaactctct
      661 atgccacatt catacaaccg catctggatc catgcgacct ggtcaacaaa agaccgcagg
      721 aaaatcattc acagctcatt tgagcgagag atgtaccgct acatgtacaa tcaattcaaa
      781 caatgtggtt gcgctgtcaa aatcattaac ggtgttgaag accatgttca ttgcctcttc
      841 ctcctgaatc caagcaaatc ggtcgctgac gtgataaagc aggtcaaagg cagtacatca
      901 cactttatca atgccggaga ttttgatatc aaggatttcg catggcaaaa cgggtatggt
      961 tccttttccg ccagcgaatc agttacagat tatattgcaa gctacattga aaatcagaaa
     1021 catcatcaca aaagaaaaac gtacgacact gaatttaccg gatttctggc ggaaaatcac
     1081 attgatccaa tggacaacga atgaccggtt ttatcatgaa agtgtgcaac agcttgttca
     1141 aaaataatcc ttaaccgaac aaattcagct gtaaatttca attacttttg tcctccgaat
     1201 gctttacgat tttaatttca atcagcatcc tttatggatg attgtggtgg ggggcgctct
     1261 ggttctttca ctactggtgt ttttatttta ctacctgttt tttttcagac gttttgcatt
     1321 ttataaaaca aagcatcatc aaacagatga aaagaaactc cctcctgttt ctattataat
     1381 tgcagccaaa aatgaatttc tgaatatccg ggaaaatctt ccattccttc ttaatcagga
     1441 ctatccggaa tttgaaattg ttattgttga tgaccattca caggacgaca catgggactt
     1501 gttgtgtgca tttaaaattc acaatccgaa tctcagaact gtccgcctga cagattctgt
     1561 ggttgtttgc gaaggaaaaa aattcccgct ttcggtagga atcaaagaag ccaattatcc
     1621 gcacctgctt ctgaccgatg ccgattgccg ccccacaagc ccgttctgga ttcagaaaat
     1681 gattgaacaa tataacaacg gaacagaaat cgttgttggt tatggaaaat actaccgcca
     1741 caaaacattg ctgaatctgt tgattcgttt cgatacgatg cgcatcgcac ttcaatattt
     1801 ttcggctgcg ttatcgcatt ttccatacat gggagtaggc cgcaacctgt catataaaaa
     1861 agaactattt atcaacagca acggattcac caatcattac gatctgagct ccggcgatga
     1921 cgatctgttt attgcagaac atgctgaaaa aaataataca gctatctgca ttgaatccga
     1981 tgcatacacc tggtcggagc ccaaacacag cttcgcatca tggtggcatc aaaagcgcag
     2041 acacctgaca acaggcaaca ggtaccgatt caaacacaaa ctgtatcttg gattatatca
     2101 attgaatctg ctgctgttcc ctgctctcgc agctctcttg ctggcatttc aatatcagat
     2161 tcttgctgtt gcatccgttg ttttaattaa atggtcaacc caatggataa ttttttcagg
     2221 aacaacaaaa aaactcaaag aaaaaaaatt attattaatt tcgcctgttt ttgaaacaat
     2281 aatggcggta ctgaatgcgc tattatatgt atcgaacatc atcagaaaac ccggcacatg
     2341 gaagtaagtc cgaattttac cgaaaaagcc aaacgggatt atgaactcgt tcagcgggca
     2401 ttggatcatg gtgatcagaa ggcctacact gaactgatga aaaattaccg tgaagccata
     2461 tacttcatga tgctgaagat gaccaatgat gccaccgatg ccgatgatct cactattgaa
     2521 gcatttggca aggccttccg caaccttcct caatacacac ctgaatttgc tttcagcacc
     2581 tggcttttta aaattgcatc caacaattgc atcgattata tccgcaaaaa acgcctggta
     2641 gttaccagta ttgatcaggg tgtgcacaac gaagacggtg gaatggctca gaacattcct
     2701 tgtgagcatc ccgatccgga agaagccatt atcaaaaaac aaaaacacaa ggcacttcgt
     2761 gaagtggttg aaaaattaaa accacattat cgcagactgg tagaactacg atattacaaa
     2821 gaatacagct acgaagaaat tgccgaagaa ctcaacctcc ccctgggaac tgtgaaagca
     2881 caactcttcc gcgcacgcga cctgttatgc cagatattat caaaagacca ggattcaatc
     2941 taatggccat acaactacac actttcgtct ttaatggttt tggcgtaaac aactacattc
     3001 tcgaagcaga gaacggtgat tgcattatcg tcgatgccgc atgctccaac gattccgaaa
     3061 aggaacaact gaaaagcttc ttcagcgaca gcaggcgcaa acctgtgatg ctgataaaca
     3121 cacatacgca tatcgatcat gtgcttggct gcgcatttgt tcagaaaaaa ttcggaattc
     3181 catggctcat gcacagcgag gcactgccaa cattaaacga agctccggtg tttgcctcac
     3241 ttttcggaat caataatatg gaaatgtcga aaccggacaa ttttctcgaa gacggccagg
     3301 aactggtttt cgacaacaac acaataaaaa ttctgtacac acccggccac gcaccaggat
     3361 ccatctgcct ctacattcct gccgaacgca tggtcattac cggcgatgta ttgttcaatg
     3421 gcagcatcgg ccgcaccgat ctcaccggag gcaacttcga tgtgctgatg gaatccattc
     3481 acacaaagct gctcaccctg cccgacgaca ccatcgtgta tcccggacac ggccccgcca
     3541 ccaccatagg tgacgagcgg actgggaatc cgtatctggg gtaagttgag actgtctcaa
     3601 aatggcctaa taaatagacc taaacttcta attgcaaacc gaccttccag agtcctcaat
     3661 gcaatggaga acctggaaga gtcggatgct gtggcctctc accaactatt ccatgttttt
     3721 cgattccctt caaactatgg aatgttggat tgatccccat tgttttgaga catcctttca
     3781 caacagaaat atttgtattt ttgtttctca tctccgccat gcttcaatcg cttcacatac
     3841 agaactacgc actaatacgt cagcttgatg tcgatttcgc atcggggttt acggtcatta
     3901 ccggagaaac cggcgccgga aaatccattc tgctcggagc gattggtctg cttattggaa
     3961 accgtgccga tcatggcgta ttactcaacg aacagcagaa atgcatcatt gaaggacaat
     4021 ttgcacttga aaaaaatgca tttgaaaatt tcttttcaga caatgaaatt gattttgaag
     4081 gagaactgat catccgccgc gaaataagtc ccggtggaaa atcgcgcgcc tttgtcaatg
     4141 acactcctgt gcagctttcg atgctgaaag atctcggtga tcagatcatg aacattcatt
     4201 cgcagcacga aacactgctg attgccggca atcgttttca aatgcagaca ctcgattcgt
     4261 actgtggcaa caacgcgctg ctggccgaat acggagaata tttcaaaaaa tacagcgatt
     4321 gccagcatcg tatcaatgag ctgaaagaac agattgcacg acgcctttcg gatgccgatt
     4381 attttggttt tcttttcaaa gagcttgtcc aggcctcgct ttcagcagaa gaatttgcat
     4441 cgttggagca ggaacaaatc atgctgatga atgccgaaaa aatcggtgaa ggacttttcg
     4501 cctcgttgca gattttatct gaaagcgatg ataacgtaca aatcacactg cgccgtgttg
     4561 agcaatattt aaaaaacgca tcgcagtatt tgcccgagct gactgaattg tcagaacgca
     4621 tcaatcagtc gctgatcgaa atttcggata ttgccgacac gctgaaccgc atgagcgata
     4681 aagcggaaat caatcctgcg cgacttgaaa aagtgaatga acgtgtggat gaatacaacc
     4741 ggctgatgca taaacacaag gtttcagaca ttgagcaatt gctcgccatc aaggatgaaa
     4801 tcgaagcaaa gctggaggac aattcaaacc ttgaactgaa gctggaatcc gaggaaaaaa
     4861 tgctgacaga actggacaag gagttgaaga aaatttctgc cgatcttgat aaaaaacgca
     4921 gaaaagccgc tcccgctttc cgcgacgcca tacttgaaat tctgaataat ctcggcatgc
     4981 cgaacgcctt gtttgaagcg gcacttgaga ataccgactc atacaaacca gatggcacaa
     5041 acagcattcg ttttatgttt acagcgaata aaggagagaa gctacaggaa cttggcgctg
     5101 tggtatccgg cggtgaaatg tcgcgcgtga tgctcgccat caaatcgatg acagcacaac
     5161 gcaacatgct tcccaccatc atttttgacg aaattgacag cggcgtttca ggactgattg
     5221 ccgccaagat gggaaacata ttaaaaaaac tttccgaaaa ccatcaggtg attgcgatca
     5281 cacatctgcc acagattgcg gcacgcggcg aagatcacat tcatgttgaa aaaaatgatg
     5341 ccggcgaaaa aatgcagaca atactttgca agcttgattc agacgcaaga gtagaggtga
     5401 ttgcccgtat gatctccgat gaaaaaatga gcgactctgc gctggaaatg gcgcggcaac
     5461 tgctttccaa ttaacaactc aaaaacaaaa atcatatgcc ttcaaaaaaa tactttctcg
     5521 acaccgacaa aaaagagatg ctggatttat cttggaaagc aggattccat actgttacgg
     5581 tatcatataa tggcattttg ctttcaacca tgaaccgcga agaagcatca acgggaaaag
     5641 cagtagagct gcccgatggc cggattgttg atatcaagct ggaaagtgga tttttcgctg
     5701 cattgacagc aaagatcaac ggccgtcata tcccgggaac acaaggtgat ccgaaatatc
     5761 aactgaaaca ggttttttat ctgatgatta ttcttggcat actcaacatc atcatcgggt
     5821 tgatttttgc cgtaacagat gtccatatcg acggactgga aagcatcggc tatattaatg
     5881 tagccatcgg acttgtatat attgcccttg gatatgctgt aatgcaaggc agcatgattg
     5941 cgctggttct gataacactc attctgtttg gcgatcttat tctggccgcc atgtattcgg
     6001 ctcagtcggg catgaccgct ggaattatca tgaaagtatt tttcgtgatt tttgttgtgc
     6061 gcggattcaa atacatgaaa gaattcagag cggagagaca tgtgcaaagt tgaaactttg
     6121 catagctgga tcgctgattg aaactttgca caggaaaaca aaaaagggct gcaaacattt
     6181 gcagcccttt tttgttgcgg ttttccgaag atcaatacac gatgatattt tgttctttcg
     6241 gaaatttcac ggagtatttg atggtgagtt ttttcgtttc acccggcttt agtttcagat
     6301 tccacgacac tttcccagtg ttttcgtcaa caatgcctcc actgaattca attttttcga
     6361 tttcaatctc tttattggtt gtaatcggga actggtcatc gattacgata tcaatttcct
     6421 tacttttatt gttgcgaaca gtaatctccc agcccttggt gaccttgcgt gaggtgccga
     6481 taaatttatc actggtgtaa tctttaacca tttcacgttt tattgaaatt gatttatcgc
     6541 gacccagcga gacctcaagt gtatcgagcg ctgtaagcgg atttacataa gactttccaa
     6601 cataggtttc ctgaaaaaac acattggatt cgcccggcaa cagattgtat ttttcccagc
     6661 cggttacacg cgccagaagg aatgcatcat tatcaagttt cggcgcagca taatactgat
     6721 acaatgcagc caactcatat tccttcacct gaacaatacg cgctttgcca tttgtttcaa
     6781 cactataagg aagatcaata gcatattcaa aattaattgg atactccgtt accgatgtgt
     6841 aagccacagc actttcgaag tttttatctt catcatattc ctcttttgaa acttcggctt
     6901 ttgaatcagc aacagacata ggaacagccg cgcgattatt tccgctctga ctcctgtagg
     6961 agtaattatt gaaataaaga taccatggat ttaaaaccgg tttattgttg ctctgatttg
     7021 gattggctgt tgacaaagta agcttcactt tttcccagtt ttcgccggtg ctctgataga
     7081 taccagcttt gaaagtgagt ttgaccggat ttccgaaatc agtagcacga atatcataca
     7141 ttggtgccca gccggcattc tgtacgttgt agcgtgttac aaaagttgca ttgccagctg
     7201 cattggcaat aaccctgact ataatttcgc ccgagggctg attctgtttt gcattatatt
     7261 cattgagctg ttgctgaaca cgcgcgatat ctttattcaa tctggcaagc ttgattccaa
     7321 cttcaagctg ctttgtttta atatcagtca gacgaattcg ataaaagtcg gcattggcct
     7381 ttaatgcggc aatatcaacg ccactgtcat ctcccccaat ttctttattg gcaagaatca
     7441 tggcctcttc attgttataa acagtcaaca tggcattctg ataattgatc tgtccacgca
     7501 acatttcaag cgaatcttca agcacaagaa ctttctttga cttgatctga gatttcagat
     7561 agttgattct gttttcgaca gaaagcacgg taatattccc tttacccgaa acctgaatgg
     7621 tattgggatc gattgtggtg ggcaaatcct gaataacgat atcgtaatta ccagtattca
     7681 gataaatggt tcctgtgcgt tctacctgag ctccgctaag aaaaacagta acattccgga
     7741 tgctggttga aaggactttt tcatcatcgg caagaacaaa catggccgat aataacattg
     7801 taaaaaacaa aacggtcttc atgggtatag agttttaatt ttaaaaaacg gcgcacaaaa
     7861 aaaaatcctg tcgcctgaaa aaaatgttca gtgcaaagag atgctacaat aagaaaaatt
     7921 cacaaattaa cgaatcaatt ctattcctcc aaaaagcttg cgcttgattc cttccgtatc
     7981 gcgataaaaa atttcgtagg tatatgaacc cagcgcacag cgctttccat taaaggtacc
     8041 atcccagccc tgctcataat cagtggtaat aaaaagcatt ttcccaaatc ggtcgaacac
     8101 catgaacgta taaaaatttt catcaattcc atgaccaatg ggcttaaaaa cctcgttgat
     8161 tccatcacca tcaggagaaa acgagttggg cacatacata gtaacaacat ccttcacata
     8221 gattacatcg ctggcagtat ccttgcatcc atgttcagta gtaataatca gttccacaac
     8281 atacaggccg gtatcattat actgatgcgt gggataagta tccatcgaga agccgccatc
     8341 accaaaagtc cattccgatt gatacagact ggtgctttca ttgaagaaaa gaaccgtatt
     8401 atcggccaat gtaggagcag aaggataagg acggaattca gccactggtt tccgataaac
     8461 attaatcata ttatttaccc tcagcgaatc aacacagcca tagattgatg ttacagcaat
     8521 gaaaacatca aacaccccat cctgttcaaa aataagcgaa ggatttttat cactgctgaa
     8581 atgatgaccc tcgtcgagat cccagtaata agtctgacca ctatcaggcg ttgtttcata
     8641 aaactgaaca aacaatggct gacagccctc ggttggtgaa gctacaatat taatttcggg
     8701 cagcggatgc aattcaataa gtgttgtact gaccgtggta tcgcaaccaa ctgcatcggt
     8761 aattgttatt gtatatgttc cggcaggcag gttagataca gagggagcat ttacctgagg
     8821 cgggctccat gtatatgaat atggcactgc acccccactg acaacaaggg aaacggatcc
     8881 atcagaacca ccctcacagg ttacgtcaac cttgctgaca atgacctcaa tgggtggata
     8941 agcatcaata atttctgctg aatcggacac agagcaacca ttggcatcga aaacagtaac
     9001 ataataccag cccgattcga gacctgaaac agaattactg gttgaaacgg ttgttgtact
     9061 atcgcctgtt gaccagatat aagtataggg agttgcaccg ttttggacaa caattgtaag
     9121 cgttcctgtg ctatcgccaa aacacaatac gtcttctgca tcaaacgaaa caatttcaag
     9181 tcccgggcaa ctggtagcca tgctgaaacc gggaaatacc gtgtcgttaa aaccggccag
     9241 ctcccaggtg tcttcgtacc aggtaatcgg aatataacca agaggccatg tgtatgaaaa
     9301 caaaggacct atatgaattg acaatttgga ttcaaaagcc cttacctcag gaatggttac
     9361 acccggcata caggcctgct ttaaatactc attaccactt attgtatccg aaaacggcac
     9421 atcgatacac aaatccggaa tatcggcagg aggcacaatg ggaaacgacg gaatattgat
     9481 ggtgaactca agagctgcaa ttgaaaaatc aaaagagaga ctatcccagg tatgattggt
     9541 aaaatcattt gtaagcgaat gagacaggtc gaaatcaatc tgattgacat tgtggcaggg
     9601 ccagatataa ttaaggttat tacatgcctt aaaactgata ttgtctgaaa atcctgaatc
     9661 gacaaccatg tttccgctgg aaggatcagt tacggtatat tcaaccggaa ccgaaagaac
     9721 caagttattc cacacatccg gttcaaaggc aaaatcctgc tgcatagtgg ataggatggt
     9781 gaaccaggca gaaaacaaca tatagtcaat ggtgaaacct gccccaagat cataagtacc
     9841 actgagcata ccaagaaact gctgaacagc cggcccggta ggtggcggaa tcaacccggc
     9901 aatagcggat aaaaactgaa taacatcgag ttccagagaa acccaggtgc agtcaccggc
     9961 ggcatacaaa ttttgattgc attcatcaat aacatcgagc caatcggttg tttcgacata
    10021 gggaagcgta atgctgccac tcagacctat tccaaaaaga ttgttgaatg taatcggcaa
    10081 aatggtatca tgacaaatct gaataggcgg aaaggccgtc ggcacaaagc aaggatatgt
    10141 aaaaaccccg gtttgggaat taagctcaat gattgggatg gtatcaaaag gaacatttac
    10201 atcaataacc ggaaaagtag tacagccaaa aacacaaata gtggcatcga gatcaattcc
    10261 aaatccaaaa ttgagataca ggccgataac gcccgctgtg ggaaaatgtg tcgtaagatc
    10321 ccagccaggc cgcacatcat aggatgattc gatggttacc tcttcgcccg ggcaaaaagt
    10381 atcatcgttt ggaatctcca tttcaacagt taccggatat tccacatcga cagcacctgt
    10441 tgtaaagccc gatataataa aatcggaacc aatatgaaac catgtatcaa gatcgatttc
    10501 agctccgaac tgcccccccc agtacattaa cgatatcacc tatggtaaca gcagtgtcat
    10561 attgtacatc aaacaaattg atgttcatat tgatgttcca cggatttcca ttgggtcccc
    10621 acatattctg attataagtg gcccaatcaa tgtcgtttga aatcacatag ctctgagctt
    10681 ttgcattctg caatacaaaa agagagacca gaaccgtgaa caacaacttt ttcatatcaa
    10741 tcagaattca accgtaagta aacattcctt taaaacacta aaaaccacag gttttgccat
    10801 taccggatag ttggcgctaa cccagaccgt tcttttatga tcgcccgtaa gaagcatcat
    10861 tcgttcatac tcaacatctt tatccagtgc tttgataaaa aatgaaacaa tgaaaaactc
    10921 tgctttttta tcatcattgg tcaacagagt ataatgttcc tttaatgttg cattttgccg
    10981 taaaaattcc tcttcggtga ggccgccggc aatcatggaa taaatcattc cgtcaagaat
    11041 ctgaacctga atggtacttc cggcaccctt atgaataaaa ccggttttgt ctttctgaat
    11101 aaaaggcata aaataagccg gaggaataat actgacacct gtactgtcga aataaaatct
    11161 ttcattcacc ttatggtaag tggtatcaat caacatttca ggtgaattgt cctgctgtgc
    11221 cattgaagga acaacaaata cacctgaaaa aaagagcatc aaaaaaaact tcaatgtaaa
    11281 aattttaagc attttccccc tttgaaatta aaatactttg acgcaaatat atggaatttg
    11341 taaaacaatg aacagaaaat acacgatgaa ttgaacttta ttatattttt gccgcaattg
    11401 aattgccatg aaaaaagttg ccatttccat tattgtattt tttgttgcat tgcagggttt
    11461 ctgtcaaaac gactcaataa agccggatcc cggacaattt tttgccgaca tccatacctt
    11521 ttacatttat gatgtcaatg gacaaacgaa cccccgtagt gcatttggaa tcaatacggc
    11581 cattatgggc tacaaaaaat ccttatcaaa aaaaatacag gccaccattt tgtatgatgt
    11641 aacgagaaca acaaatttta tttttcccga caccattggc atttcatcat actttgaagg
    11701 aagcaaatac acagcatttc taaaaatggc tgaaatcgga tggcaaccca aaccctggat
    11761 tgaactatat tttggacaat tactgaacga acagtatctt accatacagg ataaacactg
    11821 gggattgaga tatgtaaaca ccactgcaca ggaatatttc cgatttggga atcccgccga
    11881 ttttggtgca agaataaaat tgcgcccatc cgaaaaactg atcatgagcg tttcaataat
    11941 gaacggagaa ggtccattcc gttatcagga caacgaatca ttgtttcaat actgcatcaa
    12001 tgctgaatac aggccacaag aacatttaat ttttaaaata tacggcgatt atcagccaca
    12061 tgcatccggt ttgaacaaag accgctgcgc aataagcgtt tttgccggat ataaaaaagg
    12121 gaaatggatg gccggcgccg aatacaatcg tgttgaaaac aatatttttc tgaacaatag
    12181 cgacttgagt ggaatgagca tatacagctc attcaaaaca ggcaaaaaga caagcgtttt
    12241 tgcccgtgcc gatcatatgt tccaatacgg cgatgttgaa aacgaggcct atttcatgtt
    12301 tggagctgaa taccgaccag ctcctgcatt cggacttacc ctctcagtaa aacgaaacac
    12361 ctggctcaat actttaacac cgggcataca ggcaggcatc cggttttaaa aaaccagaaa
    12421 atttatcggc aaaaaagcac aaattaactg tgaattatgc ccattaacgg ccatcatgca
    12481 acatttttca agtaaaacaa tcttataatt taaaattatt aaggtgataa aacaactttt
    12541 attttcaatg gcagcaattc tatttgtttt tgctgcctca gcacaaactg tccgcattcc
    12601 tattcagggc gaaacaacaa aaatgaaaat tgagtcaaac aacggacttt catttacagc
    12661 cgttctgaac atcagtgaat ttacattaac caaagttgaa acaccagcgg gctatttcac
    12721 cgttgttgat ttgccacagt tcaccaaaag atttgacgac ggacgtccgg ctattccatt
    12781 gttcagcaaa ctaatcgaaa ctccaacaag agggccggcc agcattagca atataagcta
    12841 tgatattgcc gaatttgacc ttgatgatct tggcttcagc gacaaggtga ttccggcaca
    12901 gccctcatgg tcaaaaaaca cagacccttc tgaaataatt tttcattacg atcaggcctt
    12961 ttatggcatc aacagttttt caaacaacca actgatccgt attgttgaag gcggcgaaat
    13021 gcgtggtgta aacatgggaa ccgttgtcat tgatcccatt tcctacaatc cggtcagcaa
    13081 taaaatagaa atcaaataca atatccgctt cactgtcaat tttgacccaa tcggatatgc
    13141 acaatacaac caacgcaaag ccaatttctt cagtcccgca cacgatggat tttataaaga
    13201 attggccaac tatattgagc cggcaacaaa agatatcatc accacctatc caataaaatt
    13261 tgtcattgtt gccgaccgaa tgtttgaaac agcattgcaa ccctttattc aatggaaaac
    13321 cgaaaaaggt tttacagtca ttgaagcata caccgatcaa tcggccgttg gaaccactac
    13381 cacatccatc aagtcgtatc ttcagggatt gtatgatgcc ggtacaccaa ccgatccgtc
    13441 acccacctat gttttattcg ttggtgatgt agcacagatt ccggctttca acggggcttc
    13501 catgaactcg catgttaccg acttgtatta tgcctgttat ggcggatcaa cagataatat
    13561 tccggatatt tactacggac gcttttcagc gcagactgtg gctcaattga caccacaaat
    13621 caacaaaacc ctgatgtatg aaaagttcac catgccttct gccaattaca tggatactgt
    13681 gttgatgatt gcaggagttg atggaacata tgcaccaaca tacgggaacg ggcaaatcag
    13741 ttacggaacg aacaattatt tcaacgctgc tcacggcatt tactcacaca cctatttgta
    13801 tccagccagc gatggaacat cggttccatc tgaaatcatc aacatcatcg gagatggtgt
    13861 cggatacgca aactataccg ctcattgcag cactaccgga tggggagacc cctcatttac
    13921 aacaaccggt attgccagtc tgaacaatgc caataaatac ggactaatcg ttggaaactg
    13981 ctgtcaaagc gtcaaattcg aagacagcaa ttgttttggc gaagccattc tgcgcgctga
    14041 aaacgaagga gccgtcggat atattggcgc atcggactac agctactggg atggagatta
    14101 ttggtggggc atcggaaata catcgagcat tacatccaat ccaacctatg ctgggtcggg
    14161 actcggcgcc tatgataaag catttcatga taatggtgaa gccacttcgg aatggtttat
    14221 ctcaaacgga caaatggtag taggcggtaa tattgctgtt caggcatcga cctacagcac
    14281 aacctttaaa aagtattact gggaagaata tcacctcatg ggtgatccat cagtcatgaa
    14341 ttatttttca aagcctgatc ccctcaccat ttcctacgac aatccggcac aggttggcaa
    14401 cacttcgctt gttgtaaata ctgaagcata cacctatgtc gccatttccc tcaacggcgt
    14461 tcttctcgat gcacaatata ccggaaccaa cacctcggtt acactgactt tccctgcatt
    14521 cacttcggtt gataccgcgc tggtggttgc cactaaacaa aacaaaatac cacacatcgg
    14581 agatctcccg attgaagatg tttcggttac gctcgatgcg caggttcttt ccattctgaa
    14641 accaacagga tcctatgcct gtaccaatat ttcagaaact ccccggattg tcatcaaaaa
    14701 tcgcggcatc aacaatctga catcaactac cattaaatac aaattaaaca acgggacact
    14761 tcaaaccgcc agctggaccg gaaatctggc caccaacgag agtgacacct ttgacctgac
    14821 aaccattacc attcctgccg gaacaaacac attccttgca tataccatca atcctaacgg
    14881 cggaacggat atgaatatcg ccaatgattc agcttcaatc accttcgtag cagaagacct
    14941 tcccattgtt gccgctttta cagcatctcc caccgcattc tgttcagctc ctgcaaacat
    15001 tacactcgat aacaccagca ccaatgcttc atcgtatttc tgggactttg gcgatggaac
    15061 aacctcaacg ctggctgaac ccacacacac ctatacctca gtaggattat atactgtttc
    15121 actcactgcc aacggtggcg tatgtggctc agacagctac gacataacgg tcatggtggg
    15181 ctcacaacca cctgtcatca atgataccac agcctgtggc gctcagtcat ttgtgttaaa
    15241 tgcaggcggc agcaatatta actggtacag cgatgcaacc ggtacaaccc agatcggaag
    15301 cggaaacaca tacactactc cggtaattac aaacacgcaa acattttatc tgaattcagc
    15361 atcttcatca caatatacag gaggcaaacc cgataattcg ggtacaggtg gatattttac
    15421 caacacaaat ctacacggaa tagttttcaa ttgcacatca cccgttaccc tcaaaacagt
    15481 ggttttatat tccgacgatg caaattccaa aaccatcact ctgactgatg ccagcgataa
    15541 cgtcattgat caggccactg tttcagtcat cagcggcgaa aataccgtta cactgaatat
    15601 tgatattcct gtcggaaacg gattaaaact catgggaccg gcatcaccaa acctttacag
    15661 aaacggatca acaacaggac ccaatcttta tccctattca gtcggcccat acattgaatt
    15721 gatcggaagc accgcctcag gttatgaaac ccaatactat tattacttct acaactgggt
    15781 tgttgaagaa aattgcacaa gcgcgatgga atcagttaca tgtaccattc ttcccgttcc
    15841 tgtagccgat ttcagtgcta caacaacaaa cacatgcgaa aatactacga ttacgctgac
    15901 agatgccaca agcggttctc ccaatgcatg gatatggtca atcaccccga caacatacag
    15961 ttttgtaggt ggaaccagcg ctgccagcca gaatccacag attcaattta cagcacccgg
    16021 cacatacaat gttcaactca cagcaaccaa caactgtggg tcagatatcg aaagcaaaaa
    16081 taatctgatt acagtagcca ctccaccggc ccagcccagt gccattgccg gaaatgccac
    16141 accatgtgca aatactaccg gactcaccta caatgttacc aacgttggcg gcgtaagcta
    16201 caactggaca gtacctacag gatggagcat aacatcagga aacggcacaa ataccattac
    16261 ggttgacgcc ggtgtaaatg caggcacaat taccgtcaca ccaaacaatg gctgcgaagg
    16321 cacaccgcaa gccctggctg tagcaatcaa cacagctccc gatcaaccat cggccattaa
    16381 cggtcagatt ggtccctgcc aggcgggatc tgaaacctat ttcgttacaa atgtaccggg
    16441 agttacttac acatggagcg ttccagcaga ctggattatt aattcaggac aaaacaccag
    16501 ttccattaca acaacgattg gaacacaaag cggcaacatt acagtaacac catcaaatgc
    16561 atgcggcaac ggaacggctc agattctgaa tgttacatca tccctcatgc caaaccagcc
    16621 ctcagccatt tccggcgaca acacaccctg tcagacttcg caaacataca ccgtttcagc
    16681 cgaaagcgga attacctaca cttggtcttt cccaacagga tggaacattt tatcggggca
    16741 aggcagcaac agcataactg ttattccatc caatactgct ggtgttattt cagttattcc
    16801 ttcaaacggc tgcggcgctg gaactgctca aaccatgaat gccactcccg aagagatggc
    16861 tgaacaacca tcggcgattg caggaacaac aacgccttgc agctttacaa cacaaaccta
    16921 ttctgtaacc aatgtgacag gaattaccta tacctggaca ttgccctctg gatgggctgt
    16981 taacacaggt cagggaacaa acagcataac tgttacaaca ggaaacacca atgggactat
    17041 tacagtaaca ccttccaatg catgcggaag tggaacacca caaacacttg atgtttcatc
    17101 agccgcctat ccacttgttg atctgggcgc tgatacgtcg atttgcgaag atcagatttt
    17161 gattctcgat gccggtaatt caggatcaaa ttatctttgg tcaaatggcg ccacatcaca
    17221 aactattacc attgacgcct ctttattaac acccggaaac ggttacaatt atcaggtaac
    17281 ggttaccaac agcagcggat gtgccagtac tgatgcaatt tcggtaaata tcctcatttg
    17341 ctcgggcatt gatgacaatt ttgaaaccgt tgcagcaata tttccaaatc cggcacaaga
    17401 caacattact gttacctgtg caaactcaat tcagcgcatt gaaattcttg acacaaaagg
    17461 tgctgtcgtt tacaatcaaa acttcagtgg aaatgttttg aatataaatg tagctgcact
    17521 ttccgaagga ttatactaca tcagggttac aacagcttcc ggctcagatg tattacctct
    17581 gacaattgat aaataaaaca ctgggaaatt atttatgaag ccctgatttt ttcagggctt
    17641 tttttatgaa ccaaaacaaa caacatatca tttttgaaac tccgcaataa catcagaaaa
    17701 acaaatcaaa attctgatat tatagttctt tgcacgaaac ctgacagata tagccatccg
    17761 gttaacactg atttaccgga tatcaacaaa cgcgtctgca gtcatgctgt taacaccaat
    17821 atgattgata aatcagatca atcaattttt ggaagattcc gacggtctca ttgaattaac
    17881 cattccgtac ctgacggcac gggctggcgt tctttttcgt gctaccaata ttttgtccct
    17941 acgggacaat tgcagcttag tcttctgttc cttgggctcc gcattgcgag aaaaattcgc
    18001 gccgctcttg cagagctgaa ataacgccaa aacgattgat tcatcggcgc aattattttc
    18061 ggaagactcc ggaggagtca catatttgta gtttatgaat atacaaacca cgactccaga
    18121 ggagtcgcat ataaagagct gtgttctctg ataatgcctg tatatatgcg cttatgcaga
    18181 ggtagtgatt gttaagccat ttcgaaaaca tcatgctgac tatgctttac cggcataaag
    18241 aaagatggtc ataaagtcat tattatcata caaacttttc aataataaaa gaattcaact
    18301 tatttatctc attaataaga aaatataacg gcattttatg atataatcgc gattttttca
    18361 tctaactgta gataaattaa atttttcttt atatttgtaa caaaatttcc accatgaaaa
    18421 aacttttcca actcgcatta atcatgctat tccctttctg ggtaaattca caggattgtc
    18481 ccgaatactt tccgcttaaa accggcaaca gctgggagct gaccaactac aacaaaaaag
    18541 acaaaatcat aggttcaaat tcctatgcag ttaaatcggt aacagaatct gcaaccggat
    18601 acgaagccat tgtaacaaca tcggcgatca atgaaaaagg agaagttgag ggatctggcg
    18661 atctgattat gaaatgtgaa tcaggagtat tttatttaga tatgaaaaac tttcttgatc
    18721 aatcggcaat gggaaacaat ccagacatga cagtaacaat gaccgccaat gatcttcaat
    18781 atccctcaac aatgaccgaa ggcagcaatc tgcccgatgc cagcattacc tatcagatga
    18841 gttccggcgg catggttgtt atgacaatga cggtcaatat tacagaaaga aaagtgattg
    18901 gcaaagaaag cattaccaca cctgccggca catttgaagt atggaaactg accgccagat
    18961 atcagtcaaa aacgggcttt attactacaa aactctctac gattgaatat atatcaatcg
    19021 gtggtggcat aatcaagtca gaatccttca acgacaaagg agatctgatg ggatattcaa
    19081 tcctcacaaa gctcagcaaa ctgtaagaaa taccggattt taatatccgc ttcaaagctg
    19141 gcggggatac aattttttgc atctgaaaag cttttaaatt taagcagggc gtaacttcaa
    19201 aatgacgccc tgttttttat aattatcaga ctacgccctg ctgttgaaaa acagctgcag
    19261 aagcaacttg caataaatgg atggagaggg cattttgcaa agcaagacca catttcattg
    19321 atgtactcac acccgctcat gaagcggttt tccgcaatac ttgcaataca cggcatcttt
    19381 gtcatgtcct tgacgtgtac aataaggaca tgtttgagcc gtagcagctt tttcgcttcg
    19441 gataatttca ccggtcatga tgcctgtagg aacagccagg atcgcataac cacaaatcat
    19501 caaaatactt gcaagaaact gaccagtgct tgtaaccgga gaaatatcgc catacccaac
    19561 tgtagtaact gttacaatac accaataaat actacgtgga atgctatcaa aacttgcatt
    19621 gacaggccct tctatcaaat acatcactgt gccgaaaaca caaacaatca acagcacaga
    19681 catcaaaaaa atcattattt tgctactgct ggccctgaaa gcggtaagca aaacccggcc
    19741 ttcggttaat tgttttgtca acttaaaaat cctgaaaata cgcaaaaccc gcaccaaacg
    19801 aatgatcata agtgaatggg ccccggtaaa aatcaaaccc agataggttg gtaaaataga
    19861 taacaaatca attattccga aaaaactttt tgcatatttc atcgaattgg ctgtactata
    19921 ta
//
LOCUS       DAOPKE010000052        19870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2884_length_19870_cov_12.207671, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000052 DAOPKE010000000
VERSION     DAOPKE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19870
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2884_length_19870_cov_12.207671"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKY63_07920"
     CDS             <1..197
                     /locus_tag="PKY63_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPF00576.1"
                     /translation="LPGILRSYSVKPGDRVKNGDTVLVLEAMKMENNIFAERDGVVIE
                     LMGQIGAAVLEGDKLIALGD"
     gene            202..1365
                     /locus_tag="PKY63_07925"
     CDS             202..1365
                     /locus_tag="PKY63_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HPF00577.1"
                     /translation="MEFSEHFIFWAAFIVLYLVVFFIDMYVTSHRKKPMTVGIALRWT
                     ALWISIALAYGLAILFFFPQNADSEISTKSIMFQKFLAGYLTEYSLSIDNLFVFIMIF
                     SLMGVHEKNQPKLLKLGILLSIVLRILFILVGMGLVERFHWIIYVFGVILLYTAYKMA
                     FTSEDDQIDPKGNFLYKSASRMFPIDPDPDKGSFFSRIGGKLHITMMLLVFLVIGSTD
                     VLFAVDSIPAIIGVIKEGADGVLTYDEENFIAITSNVFAVMGLVSLFFALKGIMGLFR
                     YLKQGVSFILFFIGVKMMLPAIGFINEKTGEAIENFFKNNSWISLTVIVGTLALSILL
                     SIAIKEKEELSETEHKLGKMEADEPIQLAEIAALKEENERLKKEIEELKKTKN"
     gene            1372..2532
                     /locus_tag="PKY63_07930"
     CDS             1372..2532
                     /locus_tag="PKY63_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPF00578.1"
                     /translation="MGNLDFASLFPALSAFTWQNLVMIAVGATLIYLAIKKEYEPTLL
                     LPMGFGAILVNIPFGSALAQLDPSGKWAEGALSVFFEAGIATEVFPLLIFVAIGAMID
                     FSPLFRNPFLLLFGAAAQFGIFATIALATVCGFDLYQAASIGIIGAADGPTSIFVANK
                     FAPDLLGPISVAAYSYMALVPIIQPPVIRAITSKKERRIKMAGANNSKPVSRTTMILF
                     PIVVTVVAGIIAPASLALVGFLMFGNLIRECGVLNKLSLGAQNELANLVTILLGITIA
                     STMTAERFVRLDTLMIIGLGLFAFVFDTFGGVMFAKLMNVFMPKHMKVNPMVGACGIS
                     AFPMSARVIHNMGQKEDKTNFLLMPAISTNVGGQIGSVVAGGLILALIPLFA"
     gene            2551..2844
                     /locus_tag="PKY63_07935"
     CDS             2551..2844
                     /locus_tag="PKY63_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00579.1"
                     /translation="MTSQHNQTAPVSQNVNHTCCNHDSIQPVDSAHQHANQTTSTTVE
                     TPAPAKVKGSTDVDHNLSASFQVMGIGMLGIFIFMAIFFGMIKLMEKVFPHKG"
     gene            complement(2946..3959)
                     /locus_tag="PKY63_07940"
     CDS             complement(2946..3959)
                     /locus_tag="PKY63_07940"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPF00580.1"
                     /translation="MKEEILKCRDEVLASVVNDSNEVEAFRVKYLGKKGILNDFFARF
                     KDVPNENKKEIGQLINELKLTVAERLEAFQQQFSASVVVHSIDDATRPANFEFHGSRH
                     PLAIVRRRMITVFEKMGFSVEEGPEIEDDWHNFSALNFPPEHPARDMQDTFFIDTNPD
                     IALRTHTSSVQVRVMENTKPPIRSIFPGRVYRNEAISARAHCFFHQIEGLYVDKNVTF
                     ADLKHALLHFSREMFDASTDIRLRPSYFPFTEPSAEMDISCNLCGGSGCAFCKYTGWV
                     EILGCGMVDPNVLKNCGIDPEVYTGYAFGIGIERITNLIYGVKDLRMFSENDVRFLSQ
                     FRY"
     gene            complement(3976..4644)
                     /locus_tag="PKY63_07945"
     CDS             complement(3976..4644)
                     /locus_tag="PKY63_07945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPF00581.1"
                     /translation="MKKILTLFVLLSTVSIMTIAQDVPQGTKIQISTSLGDIKIVLYD
                     NTPQHRDNFIKLVKDGYFDGILFHRVITGFMIQGGDPDSRDAEPKQALGMGGPGYEIP
                     MEYNAANYHKRGALAAAREGDATNPQKASSGSQFYIVTGRLFGDKELDQIEKRRGFTY
                     TAEQRETYKTIGGYAPLDKEYTVFGEVYEGMDVVEAISKVARDGNDRPLENISMTVTI
                     ISAE"
     gene            5173..5523
                     /locus_tag="PKY63_07950"
     CDS             5173..5523
                     /locus_tag="PKY63_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00582.1"
                     /translation="MKRFTFILLTLGGLMAIMFTSCEKPEETKACITVKYTTDTTKVV
                     PFAHVRLEKYDVNVEKQCDEDGYCEHVFKMEAILDVHAWLDTSQTGNGTEMYGETTIR
                     LDPGKTVYKTVFIN"
     gene            complement(5750..6340)
                     /locus_tag="PKY63_07955"
     CDS             complement(5750..6340)
                     /locus_tag="PKY63_07955"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPF00583.1"
                     /translation="MFLEKNKYESHSGWIEVVCGSMFSGKTEELIRRLKRAQIAKLKV
                     EIFKPSIDVRYDETAVVSHDANSIPSTPVDNSRNIMILAQDVDVVGIDEAQFFDEGIV
                     DVCNYLAKRGIRVIVAGLDMDYLGKPFGPMPALMSIAEYVTKVHAICMKCGALAQYSH
                     RIVHNDKQVLLGEKDSYQPLCRRCFQEEMDRLSDLT"
     gene            6418..7230
                     /locus_tag="PKY63_07960"
     CDS             6418..7230
                     /locus_tag="PKY63_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00584.1"
                     /translation="MANEIIKNGIIGALESLVEMTANVTETDGPIHQLDIDLLQDQIR
                     KLYREVQLLDEANRQLLNAGMQMKPELRPIQQKKPAEMKPEVAVEKTVEPVDDIPVPV
                     IEHFDENPEEKPVEVISQTHPITENQTTNVAEMQTEEVGEIVQNPVPENKNGDLFTQT
                     LTIAEKLKKQDNSINQQMMGNNTVRNEKLTGSPIQNLKTAIGINDKFIFVNELFRGEM
                     KEYEAVLGQINDAVSEEAAMTVLHGNLQKKGTFEKTEIRTKLERFIQRRFLQ"
     gene            7243..8517
                     /locus_tag="PKY63_07965"
     CDS             7243..8517
                     /locus_tag="PKY63_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF00585.1"
                     /translation="MKKTITLLLLSLFVANAYAQDSAYARSIMIKLGSDAFSGRGYIN
                     GGIDSAANHIETEFIRLGLKPVEESYVQPFHIAVNNFTSVELSIDGKKLKPGYDFILN
                     AHSSSVSGEFKTIYIDESIRTSKKKLNKIIPKIKGKILFLKRYETKDKEALKWYDAVA
                     HQNPFKAAGIIVLDSGNFTYSVAMFDKLFNHFTAQLAPGAFPQKPAKTVSCNIKNDYI
                     QKFPVRNVAAYLQGQQYADSFIVITAHYDHIGMLGNAVFPGGNDNASGIAMMLDLARH
                     LKEQNFRPRYSIIFIALASEEAGLYGSTWFAEHPMMDLKRIRFLLNLDMVGTGSTGIT
                     VVNATKYKKEFQKLVDINNENKFIHGVKERGEACNSDHCPFYQRGVPSVFIYTTGNEY
                     KEYHNVNDKPEDVPMTAYNGLFKLLESFMYAL"
     gene            8529..9233
                     /locus_tag="PKY63_07970"
     CDS             8529..9233
                     /locus_tag="PKY63_07970"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00586.1"
                     /translation="MKTISFLLMLIAASSVAQTNKCDSLFDAEAGVDAMSSYVWRGIR
                     YVSSPAIQPCMEVSFKNVSVGAWSSWHFFSGWSEIDLYAGYENDYVMFGVMDYYSASN
                     YILDDYFNYHSGASGHMLEAYAGFPGNDKIPFSFLAATTFFGDSDDDDNPYYSTFLEV
                     SYLFTLKNFDSELRLGFTPAEGMYADKTSICEAAFRMEREIEFPQDFALKFISELTLN
                     PVTKGLYLVGGVGIIF"
     gene            9235..9909
                     /gene="rsmI"
                     /locus_tag="PKY63_07975"
     CDS             9235..9909
                     /gene="rsmI"
                     /locus_tag="PKY63_07975"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPF00587.1"
                     /translation="MAKLILVPVPIGNKGDITQRALDCLRDADVIYCEDTRNTHRLLD
                     MYGITGKKLIAYHQNNEHQMTQQVVRFLATMNMAALVTDAGTPGISDPGFMLVRACIE
                     NEIDVEVLPGATAFVPALAASGLPCDRFYFEGFLPLKKGRQKQLEWLSKMPVTIVLYE
                     APHRLIKLLGEMETYFNPDRKICVCRELSKMHEEYVRGTIAEVKQNFEQREAIKGEIV
                     LVVSGE"
     gene            complement(10038..11612)
                     /locus_tag="PKY63_07980"
     CDS             complement(10038..11612)
                     /locus_tag="PKY63_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HPF00588.1"
                     /translation="MKRNTLILAISTLLFSFFFYEQRAGINFTLFSLALVAMLALDDP
                     KRLKDPRWLIFAALSLVSGFFVGWHGTVISVMANITALSVLAVHTLLPKTSFFPAFIF
                     SVYSYLSSFVYMIIDLSERSSSGRPRYNPAYAPGNYRHTTEKAMEPDIQNNFQDKTSA
                     DKPKLETGAKVVIGVIGLAVLIVFISLYRKASPVFKEFTDNINLDFISFPWLAFTFLG
                     FLLMYGFFFIRHHKNLHENDVNAPAQLSAEKLNLENGSFLGFRMDMRTEAIIVMIFLG
                     LLNLLLLLLNVIDISFMASGGDLPTDVTYADFVHKGVGTLIVSVIFVIAIVMYFFRGA
                     LNFYEKSSSVRLLANIWLAQNLILLIATAFRNSVYVAEYDLTYKRIGVYVWLLLTAFG
                     LITTLYKVMKKSSTWYLIRTNGWAFFAAILLLGTVNWDILIAKYNTRNVNDFDYEYLS
                     RMNDNSLPAVYHALQQSKPEHVFSFDTDYHNSRFGITNSKDEVESRIRSFNAKYEKMN
                     WKSWNYDDWVTYNNLP"
     gene            complement(11716..12966)
                     /locus_tag="PKY63_07985"
     CDS             complement(11716..12966)
                     /locus_tag="PKY63_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF00589.1"
                     /translation="MDIFEKLDIDKTALGAYFKFSHGYFTFPKLTGEIGPHMKFRGKD
                     VLVWSLNNYLGLANHPEVRKADADGAAKYGMAYPMGARMMSGQTDLHEQLEQELAAFV
                     GREDAFLLNYGYQGMFSIIDNLVDRKDVIVYDAEAHACILDGMRLHIGKRFVYAHNDM
                     ASLEKQLQHAEKVVAETGGGILVITEGVYGMTADLGDLKGIVELKKKYKFRFLIDDAH
                     GFGTMGADGSGTDAHFGVRDQVDLYFGTFAKSMAGIGGFIAGPKKIIDFLRYNMRSQI
                     YAKSLPMPMVVGALKRLEMVRTQPELRENLWKIVHAIQDGFRAEGFDLGQTKSPVTPV
                     YIKGGVNEATNIIVDVREKYSIFCSGVTYPVIPKGQLLLRIIPTAVHSMEDVEYTLDS
                     FKKIKKKLQDGEYANMPIPDITKL"
     gene            13193..13888
                     /locus_tag="PKY63_07990"
     CDS             13193..13888
                     /locus_tag="PKY63_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00590.1"
                     /translation="MSAELSRMKKLARLLEAKANLKQDIYTNTLAALNMLKTESEFIV
                     KDLRNNMANRKRVIPLEFENKGEFEFELRFAGDVLVFFMHSNVFDIGSHHEQMRTHYL
                     RSDEDRRYCGIIHIYNFLADSFKYKRMNDSGVLIGRIFINKDNHFFIEGRREMNLVGI
                     SFESDRISSTKIRQILMASLLYTLNFDLASPPFEMVQEVNVMDFESAVDQMQLKTSKK
                     LGFKFQAEEGNIF"
     gene            13952..14026
                     /locus_tag="PKY63_07995"
     tRNA            13952..14026
                     /locus_tag="PKY63_07995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13987..13989,aa:Glu,seq:ttc)
     gene            14045..14296
                     /gene="rpsT"
                     /locus_tag="PKY63_08000"
     CDS             14045..14296
                     /gene="rpsT"
                     /locus_tag="PKY63_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPF00591.1"
                     /translation="MANHKSSLKRIRSNNTKRLFNRYYSKTMRNAIRSFRALTEKSDA
                     AQKLSPILSLIDKNAKRGIIHKNKAANLKSKLTKKVNAM"
     gene            14422..14610
                     /gene="tatA"
                     /locus_tag="PKY63_08005"
     CDS             14422..14610
                     /gene="tatA"
                     /locus_tag="PKY63_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPF00592.1"
                     /translation="MNIIVNEMGMLGTTEIIVIVLVVLLLFGGKKIPELMRGLGRGVR
                     EFKDASRGVNEDEPKKQD"
     gene            14582..15433
                     /gene="tatC"
                     /locus_tag="PKY63_08010"
     CDS             14582..15433
                     /gene="tatC"
                     /locus_tag="PKY63_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPF00593.1"
                     /translation="MKTNPKNKTETSTAMEDEDLSFWDHLHELRKYLIRSIIVFIVFF
                     IAAFSFRNILFDQFLFRLLNPDFITYQWFIALADFAGIAGAEKIVLDFNLINFRLAGQ
                     FTAHISISALAAAVLTLPFLVFQLWLFIKPALYDKERKIIARNAIVIVLLFLIGCAFA
                     YFIITPLSIVFLGNYQVSEKVSNTISLSSYLSVFNSTILLSGLLFELPVLLIVLSRIG
                     LINAQLLRRYRKHAVVGGLILSAIITPTTDPFTMSVVAIPLYLLYEISILAVAQKEKR
                     HGRKNQT"
     gene            15411..15890
                     /locus_tag="PKY63_08015"
     CDS             15411..15890
                     /locus_tag="PKY63_08015"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPF00594.1"
                     /translation="MAAKIKLDVLPYADISVIAVCAPMRDYRFIWHLNNHLNVHFTQD
                     RPFKWHHKKLKTDFDYAVFRCPEHSDVLFLNNRNENVQLIPNLATIDYFVVFIESTNE
                     SDINHWIKEIRSIPGITLLTTLSGKQLDEFRHILSEMEFQEMQRSIEAKKSKVKAFD"
     gene            complement(15945..17726)
                     /locus_tag="PKY63_08020"
     CDS             complement(15945..17726)
                     /locus_tag="PKY63_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00595.1"
                     /translation="MQRFFLIAAFVFISNIVSAQIPYSEKYPNGNVKVKGRMLIDSTR
                     IGIWKYFYESGELKAVGEYSKGTEIGVWKFYYPNGDLWKEEARLDGEVKAYYAFPDSL
                     EYKGMMKNGKMHGAWEYWWENGNKQSEQFYNEGIRTAQWKYWYEDGKEWKILSMENGP
                     VTIFDTTGRKFEEWYVADGKMNGMYRKYDFFGTVLEESMYKNDARDGQYKLFHGNAKP
                     AVEGQYIAGEKTGKWNYFYISGAKWKVVEATDGPCFSWYESGEKWEEWTHKNAVKDGL
                     YNAWFINGKKKEETFYKAGLRQGISYLWHDNGNKSFEGSFLNDSLNSYCRWWYADGKM
                     EMEGKLKMDKHDSIWKFYYPTGKMGSIGNFLNDLQHGEWTFFYETGEKWRQGNYENGM
                     RSGKWIAWFESGTKQREGDFVADKEDGYWVSWFENGHIENEGYFDMGVMDGDWKGNYQ
                     NGKLRYETSYKNGVKNGHDVFYLQNGNKLYDATYSMGMKNGFCTEYGETGKPVEEGFY
                     SMNIKTGKWTFYDKYGSKIREQMFRNGRPDGKIIEFFANGKVAATGSYDDTKRVGQWV
                     YFNEEGKPVYKVTFDKDGKKISEWRPE"
     gene            17861..18766
                     /locus_tag="PKY63_08025"
     CDS             17861..18766
                     /locus_tag="PKY63_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPF00596.1"
                     /translation="MSITKLEQMISALQGKPKKRLVAANATDSHTIEAVSAAADLGII
                     DPILVGDEKIIREICEKEKIDAGKFKIVHEAGEIPAAKKAVELINAGEADVLMKGSLS
                     TDKYMRAILDKEKGLLPPKAILTHVTIVEVPTYPKLLTISDVAIIPAPDLGQKVAITN
                     YVIKVAQAIGVEKPKVALIAATEQMSAGMPACVDAAIISKMAERGQIAGGIVDGPLAL
                     DVAIDKESVQIKKLASPVAGDADCMVFPNIESGNVFFKTATKLAHGELAAVVMGARCP
                     AILTSRGDSSKTKTYSIALACLIAK"
     gene            18775..19854
                     /gene="buk"
                     /locus_tag="PKY63_08030"
     CDS             18775..19854
                     /gene="buk"
                     /locus_tag="PKY63_08030"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPF00597.1"
                     /translation="MSEILVLAVNPGSTSTKIAVFDIHNKKLFQKNLKHSAEELAPFP
                     TITDQYEFRKNNILKELKDAGIETADIKVVVGRGGLVKPIPSGVYEVNEAMKADLKVG
                     VLGQHASNLGGLIADDIAKSIGNAKAFIADPVVVDELHDLARISGHPEFERISIFHAL
                     NQKAIAKAHAKAQGKDYKSLNLIVVHLGGGISIGAHRQGMVIDVNQALDGEGPFSPER
                     SGTLPVGALAKLCFSGKYTLDEVKKMITGKGGLVAYLGTNDAQEVENMVKDGDQKATL
                     IYKAMAYQISKEIGAAAAVLKGQADGILLTGGIAYDKGFVEWIREHVSFIAPVFVYPG
                     EDEMQALAFNGICFLKGEADVKVYA"
BASE COUNT         5881 a         4457 c         4071 g         5461 t
ORIGIN      
        1 cactgcccgg tattctgcgc agctatagtg taaaacccgg cgatcgtgtg aaaaatggtg
       61 ataccgtgct cgttctcgaa gccatgaaaa tggaaaacaa catatttgct gaacgcgatg
      121 gcgttgtaat cgaactcatg ggccagatcg gcgctgcagt tctcgaaggc gacaaattaa
      181 tagcattggg agactaagat catggaattc tcagaacatt tcattttctg ggcagcattt
      241 atagtgctgt acctggtggt ttttttcatt gatatgtatg ttacttcgca ccggaaaaaa
      301 ccaatgaccg taggaattgc cctgcgttgg acagcgcttt ggatatcgat tgcactggca
      361 tatggacttg ccattctgtt tttctttcca caaaatgcag actctgaaat cagcacaaaa
      421 agcataatgt ttcagaaatt cctggcagga tacctaaccg aatattcgct ttccatcgac
      481 aatttatttg tgttcatcat gattttttca ctgatgggcg tacatgaaaa gaatcagcca
      541 aagctcctga aacttggtat ccttttatca attgtactgc gtattctctt cattttagtg
      601 ggcatgggtc tggtagaaag atttcattgg attatttatg tatttggtgt tattctgctt
      661 tacactgcct acaaaatggc ctttacctcg gaagatgacc agattgatcc aaaaggaaat
      721 ttcctgtaca aatctgcttc gagaatgttt ccaattgatc cggatcccga caagggaagt
      781 ttcttttcgc gcattggtgg caaacttcat attacaatga tgttgctggt atttcttgtt
      841 atcggatcaa ccgacgtact ttttgcagtt gactctattc cggccatcat cggcgttatt
      901 aaagaaggtg ctgatggtgt tctgacctac gacgaggaaa attttatagc cattacctcc
      961 aatgttttcg cagtcatggg gcttgtatcc cttttctttg ctctgaaagg catcatgggt
     1021 ctgttccgtt atctcaaaca aggtgtaagc tttatccttt tctttattgg cgttaaaatg
     1081 atgctgccag ccattggttt tatcaacgaa aaaaccggcg aagccattga aaatttcttc
     1141 aaaaacaatt catggatatc gctgaccgtc attgttggaa ctctcgcatt atcaattctg
     1201 ctttctattg ccatcaagga aaaagaagaa ttgtctgaaa ccgagcataa acttggaaaa
     1261 atggaagcag atgagcccat tcaactggct gaaattgcag cgttgaaaga agaaaatgaa
     1321 cgtctgaaaa aagaaattga agaattgaaa aaaacaaaaa actaaaatac aatgggtaat
     1381 ctcgatttcg catcactatt tccggcgtta tcggcattca cctggcagaa tctggtcatg
     1441 attgctgtag gtgccacgct gatttatctt gccatcaaaa aagaatatga accgaccttg
     1501 ttgcttccaa tgggatttgg agctattctg gtcaatatac ctttcggttc tgcacttgca
     1561 cagctcgatc ccagtggcaa atgggccgaa ggtgcattgt cggttttctt cgaagctggc
     1621 attgccaccg aagtttttcc gctgcttatt tttgtcgcta tcggcgccat gattgacttt
     1681 tctcctttat ttcgcaatcc attcctgctt ctgtttggtg ctgctgccca atttggaatt
     1741 tttgcaacca ttgcgctggc caccgtttgc ggtttcgacc tctatcaggc cgcttcaatc
     1801 ggcatcatcg gtgctgccga cggccccact tccatctttg tggccaataa atttgcaccc
     1861 gatcttttag gacccatttc tgtggcagca tactcctaca tggctctggt tcccattatt
     1921 cagccacccg tcattcgtgc cattacctca aaaaaagaac gcagaataaa aatggccggc
     1981 gccaataaca gcaaacctgt ttcacgcacc accatgattc ttttccccat cgtagtgact
     2041 gttgttgctg gtatcattgc tcctgcatct cttgcactgg taggctttct gatgtttgga
     2101 aaccttatcc gcgagtgcgg cgttctgaac aaactttcac tcggtgcaca gaatgaactg
     2161 gccaacctgg ttaccatact tcttggtatc accatcgctt caaccatgac tgccgaacgc
     2221 tttgtccgcc tcgatacact catgattatc ggtctcggac ttttcgcttt tgtatttgat
     2281 actttcggcg gcgttatgtt tgccaaactg atgaacgttt tcatgccgaa acacatgaaa
     2341 gtaaatccaa tggttggtgc ctgcggtatc tccgctttcc cgatgtcggc acgcgttatc
     2401 cacaacatgg gacagaaaga agataaaacc aacttcctgc tcatgccagc catctccacc
     2461 aatgttggtg gtcagattgg ctctgtggtt gccggtggtt taattctggc tcttattccc
     2521 ttgtttgcat aaaaaatgga ggacaatatt atgacatcac aacataatca aacagcacct
     2581 gtatcacaga atgtaaatca tacatgctgt aatcatgact ccatccaacc ggttgacagt
     2641 gcccatcagc atgccaatca aacaacatca acaaccgttg aaactcctgc tccggcaaag
     2701 gtaaaaggaa gcaccgatgt tgatcacaac ctgtcagctt cgtttcaagt gatgggcatc
     2761 gggatgctgg ggatattcat ctttatggcc atattcttcg gaatgatcaa actaatggaa
     2821 aaagttttcc cgcataaagg gtaagctttt tcgaaaatat caaaaaaacg ctccggaaac
     2881 ggggcgtttt tttttatttt acaatccctc gaaaggttct aaaacccttc gagagattaa
     2941 tatctttagt agcggaactg cgacaaaaag cgcacatcgt tttcagaaaa catccgcagg
     3001 tccttcacac cgtatatcag gttggtgatt cgttcgatgc cgataccgaa agcatatccg
     3061 gtgtaaacct caggatcgat accgcagttt ttaagcacat tgggatcgac cataccacag
     3121 ccgagaatct cgacccagcc agtgtattta caaaacgcgc atcccgagcc gccgcagagg
     3181 ttgcaggaaa tatccatttc ggccgatggc tcggtgaaag gaaaataaga aggcctcagc
     3241 cgaatatcgg ttgatgcatc aaacatctca cgtgaaaaat gcagcaaagc atgcttcaga
     3301 tcagcaaaag tgacattttt atccacatac agaccttcga tctgatggaa gaagcaatgt
     3361 gcccgggccg aaatggcttc gttgcgataa acgcgtccgg ggaaaatgct gcggatgggt
     3421 ggtttggtgt tttccatcac ccgcacctga accgagcttg tgtgcgtacg cagcgcaatg
     3481 tcagggttgg tgtcgatgaa gaaagtatcc tgcatgtcgc gggccggatg ctccggcgga
     3541 aaattcagtg cgctgaaatt atgccagtca tcttcgattt caggaccttc ttcaaccgaa
     3601 aagcccattt tctcaaaaac agtaatcatg cgccgtctta caattgccaa aggatggcgt
     3661 gagccatgaa attcgaaatt ggcagggcgt gtagcatcat caatagaatg tacaacaaca
     3721 gaagcagaaa actgctgctg aaaagcttcg agccgctcag caacagtgag tttcagctca
     3781 ttgatcaatt ggccaatttc ctttttattc tcattcggaa catccttgaa acgggcaaaa
     3841 aaatcattca gaataccttt ttttcccaaa tattttacac ggaatgcctc aacttcgttt
     3901 gaatcattca caacactggc cagtacctcg tcgcggcact tcagaatttc ttccttcatc
     3961 gatatctccc tgaaattatt ctgcagaaat tattgttaca gtcatactga tattctcaag
     4021 cgggcggtca tttccgtcgc gggctacttt ggaaatggct tcaacaacat ccataccttc
     4081 atacacctcg ccaaatacag tgtactcttt atccagcgga gcatagccac caatggtttt
     4141 ataggtttcg cgctgctcgg cagtataagt aaaccccctt cttttttcga tctgatcgag
     4201 ttccttatcc ccaaagagtc ttccggtaac aatatagaac tggctacctg agctggcttt
     4261 ctgtggatta gtggcatcgc cttcgcgggc tgctgccaag gcaccgcgtt tgtgataatt
     4321 ggccgcattg tattccatcg gaatttcata accaggacca cccataccca atgcctgttt
     4381 cggctctgca tcgcggctat caggatctcc gccctgaatc ataaatccgg taataactct
     4441 gtggaaaagt attccatcga aatatccatc cttcaccaac ttgatgaaat tgtcgcggtg
     4501 ctgtggggta ttatcataca aaacaatttt gatatcgccc agcgatgttg aaatctggat
     4561 cttggtgccc tgcggcacat cctgagcgat agtcattatt gacacagtgc tcagtaaaac
     4621 aaaaagagtc aggatttttt tcatgttttt caaaattgat tttccaaaat tacaaatttt
     4681 cagccatatg acgattggaa taggtgtttg attcaggggc aactcattaa aacatacaaa
     4741 taaacacatg aaccgtagtt acacagagtt tctcagaggt ttcacagagt aacgaaaaac
     4801 aaaagccgat tgaaaattac acaacgcagt tgtgttaatc aattacagca tatcgaattc
     4861 tgaaagctcg ctttcaaatt cactttgctg taattgatac cggcttttga cagattaaat
     4921 ccactctgtg cgactctgtg tgtactcaat gaaactctgt gttacaaaga ttgatgttgc
     4981 atgcttgaag ttggcattgg gtgcatcaat cagaagaatt taaaggcatt taaatgcgtt
     5041 ggccccgatg tttgatttga ctttaaacaa tttggggatt ctaaagagaa atttcaggat
     5101 tgacaactgg ttgaaaaaaa atttgtaagt ttgaaacctg aaacataaaa tttcagttta
     5161 aaaacaaaaa acatgaaaag gtttacattt attctcctga cacttggcgg actgatggca
     5221 atcatgttca caagttgcga aaagcctgaa gaaaccaagg cctgcattac tgtgaaatac
     5281 acaaccgaca ccaccaaggt tgttccattc gcacatgtgc ggctggaaaa atatgatgtc
     5341 aatgtagaaa aacaatgcga cgaagacgga tactgtgaac atgttttcaa aatggaagct
     5401 attctcgatg tccatgcatg gctcgatacc agccaaaccg gtaatggaac tgaaatgtac
     5461 ggagaaacaa ccatccgtct ggatccgggt aaaacggtct ataaaacagt ctttatcaac
     5521 taaacgatat tgttcaattt caaaaggcaa cttccatttc gggggttgct tttttatttg
     5581 ctgaaattgg aacacaacat ctctgatgaa acgcccgacc ctcaagagtt cgaaatgcaa
     5641 tggagaacct ggaagagtcg gatgctgagg cctcttacca actattccat gttttacgtt
     5701 tctcttcgaa ctatggaatg ttggactgtc aaagattttc gcagattttc tacgtcagat
     5761 cgctgagccg atccatttct tcctgaaaac agcgtcggca gagcggttga tacgaatctt
     5821 tttcaccaag caaaacctgt ttgtcattat gcacaatccg gtgcgaatac tgcgccagcg
     5881 cgccgcattt catgcagatg gcatgcacct ttgtcacata ttctgcaata gacatcagtg
     5941 caggcatcgg accaaagggc ttgcccagat aatccatatc gagcccggcc acaatgacac
     6001 gaattccgcg tttggcgaga taattacaaa catcaacaat accttcgtcg aaaaactgcg
     6061 cttcgtcgat gccgacaaca tcaacatcct gagccagaat cataatgttc cgggaattat
     6121 cgacaggcgt gctggggatt gaatttgcat cgtgtgaaac aaccgccgtt tcatcgtagc
     6181 gcacatcgat ggacggttta aaaatctcaa ctttcagctt agcgatctgt gcacgcttaa
     6241 gccggcggat caattcttcg gtcttacccg aaaacatgga accacagacc acttcaatcc
     6301 atccggaatg cgattcatac ttattttttt cgagaaacat acgcggtttt tctaatcttc
     6361 gttatctttg acatcggata tttcaacaaa aatataaaat tcaaaacact tttcggcatg
     6421 gcaaacgaaa ttattaaaaa cggaattatc ggagcactcg aatcgctggt ggaaatgaca
     6481 gccaatgtaa ctgaaactga tggaccgatt catcagctcg acatcgattt gctgcaggat
     6541 cagatccgca agctctaccg cgaagtgcag ctgctcgacg aagccaaccg ccaattgctg
     6601 aatgcaggaa tgcagatgaa acctgagctt cgccccattc agcagaaaaa accagccgaa
     6661 atgaaaccgg aagttgctgt tgaaaaaact gttgaaccgg tggacgacat tccggtacct
     6721 gttattgaac attttgatga aaatccggag gaaaaacctg ttgaagtaat ttctcaaacc
     6781 catccgatta ctgaaaatca gacaacaaat gttgcagaaa tgcaaacaga agaagtcggc
     6841 gaaattgtac agaatccggt tcctgaaaat aaaaacggag acctttttac acaaacgctg
     6901 accattgccg agaaattgaa aaaacaggac aacagcatca accagcagat gatggggaac
     6961 aacactgttc gcaacgaaaa gttgaccgga agcccgatac agaatctcaa aaccgccatt
     7021 ggcatcaacg ataaattcat ttttgtgaat gaacttttca ggggtgaaat gaaagaatac
     7081 gaagcggtgc tgggacagat caatgatgca gttagcgaag aagcagccat gacggtgctg
     7141 cacgggaacc tgcaaaaaaa agggacattc gagaagaccg aaatccgcac aaaacttgaa
     7201 cgatttattc aaagacgttt tctgcaataa tcaaattctg ctatgaaaaa aacaatcaca
     7261 ctgttactgc tttcactctt tgtagccaat gcatacgctc aggattcagc atatgcccgc
     7321 agcatcatga taaaactggg ctctgacgcc ttttcaggac gaggttatat aaacggcggc
     7381 attgattccg cagccaatca tatcgaaaca gaattcatcc ggctggggct caaacccgtt
     7441 gaagaatctt atgttcagcc atttcacata gcagtcaata atttcacatc ggtggaatta
     7501 tccattgatg gtaaaaagct taaaccagga tatgatttta ttctgaatgc acacagcagc
     7561 tccgtttcag gtgaatttaa aaccatttac atcgatgaaa gcattcgcac atcaaagaaa
     7621 aaactcaata aaattattcc taaaataaaa gggaaaattc tttttctgaa gcgctatgag
     7681 acaaaagata aagaagctct gaaatggtac gatgcagttg cacaccagaa tccattcaag
     7741 gcagccggca tcattgtcct tgattctggc aattttactt acagtgtggc catgtttgat
     7801 aaacttttta atcacttcac cgcacaactg gctcctggag cgtttccaca gaaaccagcc
     7861 aaaacggtca gctgcaatat taaaaacgat tatatccaaa aatttccggt aaggaatgtt
     7921 gcggcatatc tgcaggggca acaatatgcc gatagtttta ttgtcatcac agctcattac
     7981 gatcatatcg gcatgcttgg caatgcagta tttccgggtg gcaacgacaa tgcatcgggg
     8041 atagcgatga tgcttgatct ggcgcgacat ctgaaagagc agaatttccg tccgcgctat
     8101 tcaataattt tcattgcact ggcatcggaa gaagcaggat tatatggcag cacctggttt
     8161 gcagaacatc caatgatgga tctgaaacgc atccgttttc tattgaatct tgatatggtg
     8221 ggcaccggat cgaccggaat tactgtagta aatgctacca aatacaaaaa agaatttcag
     8281 aagctcgttg atatcaacaa tgaaaacaaa ttcattcatg gtgtgaaaga gcgaggcgag
     8341 gcctgcaaca gcgatcattg cccgttttat cagcgcggtg taccttcggt cttcatttac
     8401 acaactggta atgaatacaa agaatatcac aatgtgaacg acaaacccga agatgttcca
     8461 atgacggcct acaacgggct tttcaaatta ctcgaatcgt ttatgtacgc attataaaaa
     8521 cagaaaacat gaaaacgata tcattcctgc tgatgctcat agctgcaagc tcagttgcac
     8581 aaacaaacaa atgcgactct ctcttcgatg ccgaagcagg agttgatgcc atgagctcgt
     8641 acgtttggcg cggcatccgc tatgtaagct cgcctgccat tcagccgtgc atggaagttt
     8701 ctttcaaaaa tgtttctgtt ggcgcatggt catcgtggca ttttttctct ggctggagcg
     8761 aaatcgattt gtatgcgggt tatgaaaatg actatgttat gtttggtgtt atggactatt
     8821 attctgcatc aaactatatt ctggatgatt acttcaacta tcattccggc gccagcgggc
     8881 acatgcttga agcctatgcc ggttttccgg gcaatgataa aattcctttc agttttctcg
     8941 ctgctactac tttttttggt gattccgacg atgacgataa tccatattac agcacatttc
     9001 tcgaagtatc atatttgttt accctgaaaa atttcgattc tgaattacgg cttggattca
     9061 cacctgccga aggtatgtac gccgataaaa ccagtatctg cgaagccgct ttccgcatgg
     9121 aaagagaaat tgaatttccg caggatttcg cattgaaatt tatttcagaa ctcacgttga
     9181 atccagttac caaaggatta tatctggttg gtggtgtcgg cataattttc tgatatggca
     9241 aaattaattc ttgttccggt acctatcggc aataaaggcg acataactca acgagcactc
     9301 gactgcctgc gcgatgcaga tgtaatttat tgcgaagaca cacgcaatac ccaccggctg
     9361 cttgacatgt acggcattac cgggaaaaaa ctgattgcct atcatcagaa caacgaacac
     9421 cagatgacac agcaggtggt gcggttttta gcaactatga acatggctgc actcgtaacc
     9481 gatgcgggaa cacccggcat cagcgatccg gggtttatgc tggtgcgcgc ctgcattgaa
     9541 aatgaaattg atgtggaagt tttacccgga gccactgcct ttgtacctgc gctggccgca
     9601 tcaggactgc cctgcgaccg tttttatttt gaaggatttc ttcctttaaa aaaaggacgt
     9661 cagaaacaac tggaatggct ttcgaaaatg ccggtcacca ttgtgctgta cgaagctccg
     9721 caccggctga taaaattgct tggtgaaatg gaaacatatt tcaatcctga tcgcaaaatc
     9781 tgcgtctgcc gcgaattaag caaaatgcac gaagaatatg tgcgtggcac tattgccgaa
     9841 gtaaaacaga attttgaaca acgcgaagcg attaaaggag aaattgtttt agtggtgagt
     9901 ggtgagtagt caattaggtc aagattaagg acaaggtcag gtaagacttt cgttacacgt
     9961 tacagacgtt acaatcgtta cagattttta cttacaattc ataactcata actacaaacg
    10021 ccttacgtct ggactttcta cggtaaatta ttataagtga cccagtcatc gtaattccac
    10081 gatttccagt tcattttttc atatttagca ttgaaggatc ggatgcgtga ttccacctcg
    10141 tcttttgaat tggtgattcc aaacctgctg ttgtgataat cagtgtcgaa actaaaaaca
    10201 tgttccggct ttgattgctg caaagcatga taaacagcag gaagcgagtt gtcattcatg
    10261 cgcgaaagat actcataatc aaaatcattt acatttcgtg tgttgtattt tgcaatcaga
    10321 atatcccagt ttacagtacc caaaagcaga attgctgcaa agaatgccca accgttggta
    10381 cggatgagat accatgtgct gctctttttc atcactttat acaaagtagt tatcagtccg
    10441 aaagctgtga gcaggagcca tacatagaca ccaatgcgct tataggtaag atcgtattct
    10501 gccacataaa cagaattccg gaatgcagtt gcaatcaata atatcagatt ctgagccagc
    10561 cagatattgg ccagcagccg caccgatgaa gatttttcat agaaattgag cgctccccgg
    10621 aagaaataca tcacaatggc aatcacgaaa ataacactca caataagcgt tccgacgcct
    10681 ttgtgaacaa aatcggcata agtcacatcc gtgggcagat cgccgccact ggccatgaat
    10741 gaaatgtcaa tcacattgag cagcagcagc aacagattga gcaatccgag aaagatcatc
    10801 acaatgatgg cttcagttcg catatccatg cggaagccaa gaaatgaacc attctccaga
    10861 ttgagttttt cagcggataa ctgagccggc gcatttacat cattttcatg caagtttttg
    10921 tggtggcgaa taaagaaaaa tccatacatc agcagaaaac caaggaaggt aaaagcaagc
    10981 catgggaaag aaataaaatc aagattgata ttgtcggtaa attctttaaa aacagggctg
    11041 gccttacgat agagactgat aaatacaata agtaccgcca aaccaataac gccgataacg
    11101 actttggcac cggtttccaa ttttggttta tcagcagacg tcttatcctg aaaattattt
    11161 tgaatatcag gttccatggc cttttccgta gtatgtctgt aattaccggg agcataagcc
    11221 ggattatatc tgggtctgcc cgatgatgac cgttcagaca aatcgataat catataaaca
    11281 aacgaagaca gataagaata gacagaaaaa atgaatgccg gaaaaaaaga agttttcggc
    11341 agcagagtgt gcaccgcgag aaccgaaagc gctgtaatat tagccataac cgagatgacc
    11401 gttccgtgcc agccgacaaa gaaacctgac acaagactga gtgcagcaaa aataagccag
    11461 cggggatctt tcagacgttt gggatcatcg agagccagca tggcaacaag tgccagcgag
    11521 aaaagggtga aattgatacc agcccgttgt tcatagaaaa agaaactgaa aagcagcgta
    11581 gaaatagcca gaatcagtgt gtttcgtttc ataggtggag aattttcatt taataacgca
    11641 gattagacaa aaatatgata cactggtaaa aaaaagactg ctttatgagc agtctttccg
    11701 gaaaaaaatc atcctttaaa gctttgtaat atcagggatt ggcatattgg catattcgcc
    11761 atcctgcagc tttttcttga ttttcttgaa actatcgagt gtatattcaa catcctccat
    11821 tgagtgaact gccgttggga taatacggag cagcaattgt cctttgggga taaccggata
    11881 ggttacacca gagcagaaaa tgctgtattt ttcacgaacg tcaacgatga tgttggttgc
    11941 ttcattcaca ccacctttaa tatacacagg agtaacggga gattttgtct gaccaagatc
    12001 aaaaccttcg gcacggaaac catcctgaat agcatgaaca attttccaca gattttcgcg
    12061 gagttcaggt tgcgttctga ccatttcgag acgtttcagg gcacccacta ccataggcat
    12121 cgggagtgat tttgcatata tttgtgaacg catattgtaa cgcagaaaat caatgatttt
    12181 tttcggaccg gcaataaaac caccaatacc agccatcgat tttgcgaaag ttccgaaata
    12241 gagatcaacc tgatcgcgaa caccaaaatg tgcatcggta cccgagccat cggctcccat
    12301 tgttccaaag ccatgagcgt cgtcgatcag gaagcggaat ttatattttt tcttgagttc
    12361 aacaatacct ttgaggtcgc ccaggtctgc agtcattcca tacacacctt cagtaatcac
    12421 cagaataccg ccaccggttt ctgcaacaac tttttcggca tgctgcagct gtttttcgag
    12481 actggccatg tcgttatgag catacacaaa acgttttcca atgtgcagtc gcataccatc
    12541 caggatacaa gcatgtgctt cagcatcata aacaatcaca tccttgcggt caaccaggtt
    12601 gtcaataatg ctgaacatgc cctgatagcc gtaattgagc aggaaagcat cttcacggcc
    12661 aacaaaagcg gccaattcct gttcaagctg ttcgtgcaga tcggtttgac cagacatcat
    12721 gcgggcaccc atcggataag ccataccata ttttgcagcg ccatcagcat cggctttgcg
    12781 cacttcggga tggttggcca ggcccagata attgttaaga ctccaaacca ggacatcctt
    12841 gccacggaat ttcatatgtg gcccgatttc accggtcagt ttgggaaatg taaaataccc
    12901 gtgtgaaaac ttaaaataag ctccaagcgc ggttttgtca atgtcgagtt tttcaaaaat
    12961 atccacggta tttgtgttta aaaatttgtg caaaggtaat atttctgttt taaaacaaat
    13021 aaggaaggga tccagatagt attcatggca atttaattag attataagca tgtattaaca
    13081 tactatattg caatatacaa cgacaataga gacctgagtc attcatgaat aatgactgaa
    13141 ataatactta taattgtatt tttaagtaca tttgtaaaaa caagagaaaa acatgtcggc
    13201 agaactgagc agaatgaaaa aactggcccg cttgcttgag gccaaggcga atctgaagca
    13261 ggatatttac accaatacac tggcagcact gaatatgctg aaaacagaat cggagtttat
    13321 tgttaaggat ttacgaaaca atatggccaa ccgcaagcga gtcatcccat tggaattcga
    13381 aaacaagggt gaatttgaat tcgagcttag gtttgccggc gacgttctgg tttttttcat
    13441 gcacagcaat gtatttgaca tcggatcgca tcacgaacaa atgagaacgc attacctgag
    13501 atcggacgaa gaccgccgtt actgtgggat tattcatatt tataattttc tggccgactc
    13561 attcaaatat aagcgaatga acgattcggg cgtattgatc gggcgcattt ttatcaataa
    13621 ggacaatcat ttttttatag aaggccgacg cgaaatgaat cttgttggca taagtttcga
    13681 aagcgaccgg ataagctcta ccaaaatcag gcagatactc atggcctcat tgctttacac
    13741 cttgaatttt gatctggcct cgccaccttt tgaaatggtt caggaagtca acgttatgga
    13801 ttttgaaagt gctgtggatc agatgcaact caaaacaagc aaaaaactcg gcttcaaatt
    13861 ccaggccgaa gagggaaata ttttttaatt ttttttcaaa aaatatttgt ggattcaaaa
    13921 aaatatgtaa ttttgcagtc ccaaaacaac cggcccgttc gtctagatgg cctaggacgc
    13981 aaggttttca tccttgaaat caggggttcg aatcccctac gggctacaaa aaattaaaaa
    14041 gattatggca aaccataaat cgtcattaaa gagaattcgc agcaacaaca ccaaaaggtt
    14101 gttcaatcgt tattacagca aaaccatgcg taatgccatc cgtagtttca gagcactgac
    14161 cgagaaatca gatgcagccc agaaactcag tccgattctt tctttgattg acaaaaacgc
    14221 caagcgcggc attattcaca aaaacaaagc tgccaacctg aaatcaaagc tgacaaaaaa
    14281 agtcaatgct atgtaatttt cagcaatcat actgaacccg cccctgaggc gggtttttta
    14341 ttgcaccaga cttttgaaca tcgatagccc cggctcaaaa tcaaataatt tttgtatgtt
    14401 tgtattcaaa ctcaaagcgt catgaacatt attgtaaatg aaatgggaat gttgggaaca
    14461 accgaaataa tcgttattgt tttggtggtg ctgttacttt tcggtggaaa gaaaatccct
    14521 gaactcatgc gtggtctggg gcgtggtgta cgcgaattca aagatgcatc gcgcggagtg
    14581 aatgaagacg aacccaaaaa acaagactga gacttcaact gcaatggaag atgaagacct
    14641 cagcttttgg gatcatctgc acgagctgcg aaaatatctg attcgcagca ttattgtttt
    14701 tatcgttttt ttcattgccg cattttcatt ccgcaacatc cttttcgatc agtttctgtt
    14761 ccggctgctg aaccctgatt tcatcacata tcaatggttc atagctttgg ctgattttgc
    14821 aggcatcgct ggtgcagaaa aaattgttct tgatttcaat ctgatcaact tccgactggc
    14881 cggccagttc actgcacata tttctatttc ggctctggca gccgctgttc taacactacc
    14941 cttcctggtc tttcaactct ggcttttcat aaaaccagca ctttatgaca aagaaagaaa
    15001 aatcattgca cgtaacgcca ttgttattgt attgcttttc ctgattggat gcgcatttgc
    15061 ttattttatt atcacgccgt taagcattgt ttttctggga aactaccaag taagcgaaaa
    15121 agtaagcaac accatttcac ttagctcata tctcagtgtt ttcaacagca caatactgct
    15181 gagcggcctc ttgttcgagc ttcctgtgct gctgattgtt ctgagccgga taggactgat
    15241 caatgcacag ttgctgcgcc gttaccgaaa acacgctgta gtcggaggac tgatactatc
    15301 ggccattata acaccaacca ccgatccttt caccatgtcg gttgtggcaa ttccattata
    15361 tttgctttat gaaataagta ttctggcagt agcacaaaaa gaaaaacgcc atggccgcaa
    15421 aaatcaaact tgatgtattg ccctacgctg atatcagcgt aattgctgtt tgcgcaccca
    15481 tgcgcgacta ccgctttatc tggcacctga acaaccatct gaatgtacat ttcactcagg
    15541 accggccttt caaatggcat cataaaaaac tcaaaacaga ctttgattat gccgtattcc
    15601 gctgccccga acactccgat gtcttatttc taaacaacag gaacgaaaat gtgcagctca
    15661 ttccaaatct cgcaaccatc gattatttcg tagtttttat cgaatccacc aacgaaagcg
    15721 atatcaacca ttggataaaa gaaatccgga gcatacccgg aatcactctt ctgacaacgc
    15781 tttcaggcaa acaattggat gaattcagac acattttgtc tgaaatggaa tttcaggaaa
    15841 tgcaacgcag catcgaagca aaaaaatcta aagtaaaagc ttttgattaa agggtgtgcg
    15901 aagtttggga aagctgtgcg aagtttctaa actttgcaca atccttattc agggcgccat
    15961 tcgctgattt ttttgccgtc tttatcaaac gtaaccttat aaacaggttt tccttcttcg
    16021 ttaaagtaaa cccactgccc tactcttttt gtatcatcgt agcttccggt agcagccact
    16081 tttccatttg caaaaaactc aataatttta ccatccggcc gtccgtttcg aaacatctgt
    16141 tcgcggattt tcgagccata tttgtcataa aaagtccatt taccggtctt tatattcatt
    16201 gaataaaaac cttcttctac gggttttcca gtctcgccgt attcggtaca aaaaccattt
    16261 ttcatgccca ttgaataggt ggcatcataa agtttgttgc cattctgcag ataaaaaaca
    16321 tcatgtccgt ttttcactcc gtttttataa gatgtttcat aacggagttt gccgttttgg
    16381 taattacctt tccaatcgcc atccatgaca cccatatcga aatatccttc attttcaata
    16441 tgaccgtttt cgaaccagct gacccagtaa ccatcttcct tgtcagccac aaaatcacct
    16501 tcacgctgtt tggtgcctga ctcaaaccat gcaatccatt taccgcttcg cataccgttt
    16561 tcataatttc cctgccgcca tttttcaccc gtttcataaa agaaagtcca ctcgccatgt
    16621 tgcagatcat tcagaaaatt tccgatactg cccattttcc cggttggata ataaaatttc
    16681 cagatgctgt cgtgtttatc cattttcagc ttaccttcca tttccatttt tccatcagca
    16741 taccaccatc ggcaataact gttgagtgaa tcattaagaa atgatccttc aaaactttta
    16801 tttccgttat cgtgccacaa ataactaatc ccttgacgca gtcccgcttt ataaaaagtt
    16861 tcttccttct tttttccatt aataaaccag gcgttgtaaa gaccatcttt taccgcattt
    16921 ttatgtgtcc attcttccca tttttcgccg gattcatacc atgaaaaaca aggtccatcc
    16981 gtagcttcca ccactttcca tttggctccg ctgatataaa aataattcca tttacctgtt
    17041 ttttcgccag cgatatactg accctcaact gccggttttg cgttaccatg aaacagctta
    17101 tactgaccgt cgcgagcatc gtttttatac atagattctt cgagcacggt tccaaaaaaa
    17161 tcatatttac gatacatgcc attcattttc ccatcggcaa cataccattc ttcgaatttg
    17221 cggccggtag tatcaaaaat agtcaccgga ccattttcca tgctcaggat tttccattcc
    17281 tttccatctt cataccaata cttccactga gccgttcgaa ttccttcgtt ataaaactgt
    17341 tcgctttgct tgttaccatt ttcccaccaa tattcccatg caccgtgcat ttttccgttt
    17401 ttcatcattc ccttgtattc aagcgaatcg ggaaatgcat aatacgcttt cacctcgcca
    17461 tccagtctgg cttcttcttt ccataaatcg ccattgggat aataaaattt ccatacccca
    17521 atttcggtac ctttgctgta ttcaccaacg gccttcagtt cgcccgattc atagaaatac
    17581 ttccagatac caattcttgt gctgtcaatc agcatacgtc ccttaacctt tacatttcca
    17641 ttcggatatt tttcagaata aggaatctga gcagaaacaa tatttgaaat aaaaacaaat
    17701 gctgcaatca aaaaaaacct ttgcataatg tcggcattga ttattgtaaa atgcaaactt
    17761 agtgaaaaaa ttgcgttgaa gcccgtagtt ttttttcagc ggcaaagcaa atttttatat
    17821 cttagtcgcc taaaaataaa tttaaatttc aggaggaaaa atgagcataa cgaagcttga
    17881 acaaatgatc agcgccctgc agggcaaacc caaaaaacgc cttgttgcag ccaatgcaac
    17941 tgattcacat accatcgaag cagtatcagc cgcagctgat ctcggaatca tcgatccaat
    18001 ccttgtaggt gacgagaaaa tcattcggga aatttgtgaa aaagaaaaaa ttgatgccgg
    18061 aaaatttaaa attgtacatg aagcgggcga aattccggca gcaaaaaaag ccgtagaact
    18121 tattaatgcc ggcgaagccg atgtattgat gaaaggatcg ctcagcacag acaaatacat
    18181 gcgggccatt ctcgacaaag aaaaaggttt gctccctcca aaagccatac ttactcatgt
    18241 aacgattgtg gaagtgccca cttatcctaa gcttcttaca atcagcgatg tggccatcat
    18301 ccctgctcca gacctcggac aaaaagtggc cataaccaat tatgtcatca aagtagctca
    18361 ggccattggt gtcgaaaagc ccaaagtggc tcttattgct gccaccgaac aaatgtcggc
    18421 cggaatgcct gcctgtgtcg atgccgccat catcagcaaa atggccgaac gtggacaaat
    18481 tgccggagga attgtcgatg gaccgctggc actcgatgtt gccattgaca aagaaagcgt
    18541 acagattaaa aaacttgcta gtcctgttgc cggagatgct gattgcatgg tgttccccaa
    18601 tattgaatcg gggaatgtct ttttcaagac tgcaaccaaa ctggctcatg gagaacttgc
    18661 agctgttgtt atgggagcac gctgcccggc cattctcact tcgcgtggcg acagttccaa
    18721 aacaaaaacc tactcaatcg cacttgcctg tttaattgct aaataattct ttttatgagc
    18781 gaaattctgg ttctggccgt caatcccggc tccacatcaa caaaaattgc cgtttttgac
    18841 attcacaaca aaaaactttt tcagaaaaat ctgaagcatt cagccgaaga gttggcaccc
    18901 tttccaacaa taaccgatca gtatgaattc agaaaaaaca atattctgaa agaactgaaa
    18961 gatgccggaa ttgaaacagc cgacattaag gttgttgttg ggcgcggagg tttggtaaag
    19021 ccaattccat caggagtgta cgaagtgaat gaggccatga aagccgatct gaaagttggt
    19081 gttttaggac aacatgccag caacctggga gggctcatcg ccgacgatat tgccaaaagc
    19141 atcggaaatg ccaaagcgtt tattgccgat cccgttgtag ttgacgaatt gcatgatctg
    19201 gcgcgcattt caggacatcc tgaatttgaa cgcatcagca tttttcatgc attgaatcaa
    19261 aaggccattg caaaagcaca tgcaaaggca cagggaaaag attacaaatc attgaatctg
    19321 attgttgtgc atctgggtgg cggaatcagc atcggcgctc atcgtcaggg aatggttatc
    19381 gatgtgaacc aggcgctcga cggcgaaggc cctttctcgc ccgaacgcag cggaaccctc
    19441 cctgtcggag cgctggccaa actttgcttc agcggaaaat atacgctcga tgaggtgaag
    19501 aaaatgatca ccggcaaagg cggactggtg gcgtatctcg gaaccaacga cgctcaggaa
    19561 gttgaaaaca tggtgaaaga cggcgaccag aaagcgacgc tcatatacaa agccatggcg
    19621 tatcagattt cgaaagagat cggtgccgca gctgctgtgc tcaaaggcca ggccgatggt
    19681 attctgctta ccggtggaat cgcttatgac aagggctttg tggaatggat ccgcgaacac
    19741 gtttcattca tcgctccggt gtttgtgtat cccggcgaag atgaaatgca ggcgctggcc
    19801 ttcaatggca tctgcttcct caaaggcgaa gctgatgtga aagtgtatgc ctgatacctg
    19861 caatgaaatt
//
LOCUS       DAOPKE010000053        19635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2936_length_19635_cov_12.705209, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000053 DAOPKE010000000
VERSION     DAOPKE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2936_length_19635_cov_12.705209"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /gene="fusA"
                     /locus_tag="PKY63_08035"
     CDS             complement(<1..1994)
                     /gene="fusA"
                     /locus_tag="PKY63_08035"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPF00598.1"
                     /translation="MSNISKLRNIGIAAHIDAGKTTVSERILFFTGVNRKVGETHDGA
                     ATMDFMKQEQERGITIASAAITAFWKDHQINLIDTPGHVDFTIEVERSLRVIDGMVAV
                     FCAVGGVEPQSETVWNQADRYRVPRIAFVNKMDRTGADFSAVVEQMNKYLDASALPFQ
                     LPMGVEEDFAGMVDLIHQRAYFFDGDQRVENDIPAEFTEAALAGRQRIVEVLADYDET
                     IGDLFLNEQEVSDELLMKAAREATIKLLITPVFCGAAYKNKGIQLLLDAVIDFLPSPV
                     DIGAVVGSDVDNSEKMLTRGPSSKEPFSALAFKLINDPYVGQQTFLRIFSGTLSSGMA
                     FYNSTKSKPERAGRILRIHAKDRVEVSEAGPGEIVALVGLKLTRTGDTLCDPNNKIML
                     ESIHVPPAVIDLKINPAIRKDRDKLSEALARLCNEDPSFRAHFDQETEETIVSGMGEL
                     HLEIIMDRIKEEFKVDVEVGEPAVSYREALTGEVESNYRHVKQSGGKGQFAHTVIRFE
                     PNEGKGFEFIDKIKGGAIPAEYISSVEKGLRKALDKGPLAGFPVVDVKAVLVDGGFHP
                     VDSSDMAFQLCAAMCFKQGFMKATPKLLEPIMKLEVNTPDDYIGAVTGDLNKRRGRIE
                     AMRRYRKGSQKLNGFVPLSEMFGYATTLRNLSSGRANYSM"
     gene            complement(2371..2880)
                     /locus_tag="PKY63_08040"
     CDS             complement(2371..2880)
                     /locus_tag="PKY63_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00599.1"
                     /translation="MRSKLQIFFLTAILGIAFFGCIEQREYPIEPAITFKSFATMKSV
                     AGHDSIGYLTIEFTDGDGDVGLAQSDTLPPYNPGSNFYYNFFITFFQKTGNDFQEITT
                     PYNSRIPDVNPEHIDKDLVGDISIEIDLNILSLILVSDTIKMKAYMLDRALNESNMIE
                     TPAITLDFP"
     gene            2917..3906
                     /locus_tag="PKY63_08045"
     CDS             2917..3906
                     /locus_tag="PKY63_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPF00600.1"
                     /translation="MTGLEILSKSIFSIQTSAEFEHAALALFSWHYKHNGIYRIFCNN
                     TEKTPENVCSLQQIPCLPVNLLRFNKILPDGVSAELFFETSGTTAAETGRHYIVDPGL
                     YLASAMNCFRNFYGSIEDYVVLALLPGYLERQHSSLVYMVDHWIKCSNKPESGFYLYD
                     HEKLFSTLNSLQNKKQKTLLIGVTHALVDFSEKYSLGFPKLIVMETGGMKGRRKELSR
                     SELHAVLKKSFGVPAIHSEYGMTELLSQAYSKADGIFHCPPWMQVLLRDINDPLSPPQ
                     NKKSGAINIIDLANMYSCPFLATDDGGVLHDDGSFEITGRIDFSVIRGCSTMI"
     gene            4191..4835
                     /locus_tag="PKY63_08050"
     CDS             4191..4835
                     /locus_tag="PKY63_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00601.1"
                     /translation="MDIPKTEPDTQSPVFHIPIDSISDLIRIDQNSHTDLCQTAEPPY
                     PFKGIRKTQFDTLWEKWNIDLYNINQYKNLERFFSADAAYHKLIDLKLKNPELIFASK
                     PADVLLGIDPVSANRWNYICDSLNVHNFIQYKSNLFVFALYAFIIQSNGEIVYPRIYY
                     QLPENPLDLDTETDNLKSIMQVSGTTVKKDYVVLYFKYGYTLGTPNFYFHGEKY"
     gene            4955..6964
                     /locus_tag="PKY63_08055"
     CDS             4955..6964
                     /locus_tag="PKY63_08055"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPF00602.1"
                     /translation="MTPEEFKKAILAGIPAELPAPKPYNTNANHAPKRKDILTKKEKQ
                     LAIRNALRYFPESWHATLAPEFAEELKKYGRIYMYRFMPEYEMYARPIEEYPAKSPQA
                     AAIMVMIQNNLNPAVAQHPEELVTYGGNGAVFQNWAQYLLTMQYLANMTDEQTLTMYS
                     GHPMGIYPSHKDAPRVVVTNGLVIPNYSSRDDYERMNAMGVSQYGQMTAGSFMYIGPQ
                     GIVHGTTITVLNAGRMKAAKGEGLGGMIFVSSGLGGMSGAQPKAAKIAGVVGIVAEIN
                     PAAANKRYDQGWVDEIFSDLHALTKRAVEAVKNKEALSIAYVGNIVDLWEHLYKENIF
                     IHIGSDQTSLHNPFAGGYYPAGMSFEESNIMMAEQPEMFKEKVYESLRRQAAIINKHA
                     ERGTYFFDYGNAFLLEASRAGADIMKEDGTFRYPSYVQDIMGPMCFDFGFGPFRWVCT
                     SGKPEDLEISDNIAADVLEKMAATSPNEISQQMRDNIHWIREAQKNKLVVGSQARILY
                     ADADGRIKIAEAFNNAIKEGKISAPIVLGRDHHDVSGTDSPFRETSNIYDGSSFTADM
                     AVQNFVGDGFRGATWISLHNGGGVGWGEVINGGFGMVLDGSADADRRLKSMLFWDVNN
                     GISRRNWARNPNAIFAIKRAMEANPELSVTLPNLVNDSLLDKLDL"
     gene            7244..8041
                     /locus_tag="PKY63_08060"
     CDS             7244..8041
                     /locus_tag="PKY63_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00603.1"
                     /translation="MHQFNQIASVVPLIIAGAAIIILLIRYFLLRQIHHFFLMLVFVL
                     LSFSYIYIWLFCQQEYETITWLFPLRQPVKILLIPACYFYIAATFFGRKLNCGITLLH
                     ILPSLFIFLGFIPFWFVSPEVQINYLSTDYQNITHNPIIENVQILRLLTVFIIFNAML
                     IAYTWLAIHEIKKYKKTNNVSLFSLKGSNTFSIILVFLGLLLVLFDNAYFLGITKTPV
                     SEIVFSILSSVLLMLIFIHGYMQKECITCKKNTRKNTNNFATSKTND"
     gene            complement(8042..>8266)
                     /locus_tag="PKY63_08065"
     CDS             complement(8042..>8266)
                     /locus_tag="PKY63_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00604.1"
                     /translation="ETITGLITATALVYPDKKISSVKPKSVVKRMKEVHFAASVKREN
                     ILECEIIGLPIDTFATMALGAMAEIEQELI"
     assembly_gap    8267..8366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8580..8768
                     /gene="rpmG"
                     /locus_tag="PKY63_08070"
     CDS             8580..8768
                     /gene="rpmG"
                     /locus_tag="PKY63_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPF00605.1"
                     /translation="MAKKSKEARVQVILECTEHKNSGVPGTSRYVTVKNKKNTPERME
                     LKKYNPILKKVTVHKEIK"
     gene            8777..8929
                     /locus_tag="PKY63_08075"
     CDS             8777..8929
                     /locus_tag="PKY63_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPF00606.1"
                     /translation="MAKKVVATLKTGQTRGYTKAIKMVKSEKTGAYCFKQRILPADEA
                     QTFFKK"
     gene            complement(8942..9946)
                     /gene="murB"
                     /locus_tag="PKY63_08080"
     CDS             complement(8942..9946)
                     /gene="murB"
                     /locus_tag="PKY63_08080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPF00607.1"
                     /translation="MKIIRDISLKSFNTFGIDVKAACMAEIENADDLLNLTDALLPEP
                     FFCIGQGSNLLFTSDFKGTLIRFVYAGWKVVSQNDNEVIIRANAGCIWDELVEQTLIQ
                     GYFGLENLSLIPGTVGSTPVQNIGAYGAEASQFIVSVHVFDREAVKFFDIENKDCDFG
                     YRHSIFKSKPSWMVVSVDYRLKTVPSTSTHYKAVEDYLQKNNLDGSDPMVVRDAVIAI
                     RESKLPDYKLIGNAGSFFRNPVVEKKLFEQLHKKFPEMPFYTEADERYKIPAAWLIEK
                     SGWKGFQGFNAGVHDKQPLILINLGGASGQEIKMLAEEIQTSIKNNFGIQLHPEVIYL
                     "
     gene            complement(9943..10356)
                     /locus_tag="PKY63_08085"
     CDS             complement(9943..10356)
                     /locus_tag="PKY63_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPF00608.1"
                     /translation="MNEIEHDGVVDAVDGNVLKVRILTVGACVSCQMKKVCNPSDMKE
                     KVFDVPVEDPHSFKTGDHVKLSISEGMGILAAILGYVVPVILLMAGVLGGLSYGLNEL
                     QAAGIGLGAAFVYYVILFFTRKNASKVFVFKVSRK"
     gene            complement(10449..11306)
                     /locus_tag="PKY63_08090"
     CDS             complement(10449..11306)
                     /locus_tag="PKY63_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00609.1"
                     /translation="MKWLVFAFFLLNNASLMIAQDTLVTWDDEEILGKVVKIENKIIY
                     VILVDEPHKTYRIHSGEFQRLTYENGQTIYFFEKYPDIVTKMKKASPYKNMIEAGPFG
                     IPFNHFYVGYERLFEKRNTMEVQAGIIAPMLMTGKEKLRGGHVNFAWKRIFSEPVKIS
                     GLSVRPKMQGAYAGLMLTLNAVSYSQSIDYPVDTVGFNILYDPVKTRVPVFIPTLHFV
                     FGYNKLIAPAVMMGFCGGIGGGPRMIMSSDEHIRDQASLPETAIGIQRFASGPFSLIA
                     QISIGVLFK"
     gene            complement(11311..12189)
                     /gene="folD"
                     /locus_tag="PKY63_08095"
     CDS             complement(11311..12189)
                     /gene="folD"
                     /locus_tag="PKY63_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPF00610.1"
                     /translation="MNLIDGKLISEQIKEEIKKEVAAMIDRGERPPHLAAVLVGEDPA
                     SQTYVASKEKNCTSVGITSTIYRMDETTTEKQLMDVIEFLNKDPEVDGFIVQLPLPEY
                     FDENKVIGAINPEKDVDGFHPVNIGKMVLGQDTFISATPFGIMEMLRRSQIETVGKHC
                     VVIGRSNIVGTPMSILMSRTSNPGNSTVTLCHSKTQNLKEIASTADILIVAIGKPNFV
                     TADMVKPGAVVIDVGMHRIEDASKQSGFRLTGDVDFPEVSKKASWITPVPGGVGLTTI
                     AALLMNTLKARRKAKL"
     gene            complement(12346..13680)
                     /gene="ffh"
                     /locus_tag="PKY63_08100"
     CDS             complement(12346..13680)
                     /gene="ffh"
                     /locus_tag="PKY63_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPF00611.1"
                     /translation="MFEGLSDRLDRAFKMLKGQGRITEINVAETVKEIRKALLDADVN
                     YATAKQFTDTVKEKALGQDVLKAVSPGQMMVKIVHDELVSLMGGDKVEINLKPNPSVI
                     LMSGLQGSGKTTFSAKLASYLKSKKGRQILLVACDVYRPAAIDQIKVLAEQIGVQVYT
                     EEGVTDPVRIAKNAVAHAKQYGINTVIVDTAGRLAVDEEMMKEIRNIHDAVNPAETLF
                     VVDAMTGQDAVNTAKAFNDVLNFDGVILTKLDGDTRGGAAISIKAVVNKPIKFVGIGE
                     KMDAIDVFYPQRMADRILGMGDIVSLVEKAQEQFDEDEARKLQKKLAKDQFTFDDFLK
                     QIHQVKKMGNMKDLIGMIPGIGKAMKDVEIDDNAFKGIEAIIYSMTPEERTNPSLLNG
                     SRRMRIANGSGTTVAEVNKLVKQFEDTRKMMKMVTSGGARNAMKQMKQMRRH"
     gene            13829..14368
                     /locus_tag="PKY63_08105"
     CDS             13829..14368
                     /locus_tag="PKY63_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPF00612.1"
                     /translation="MKTTIIILAAFISTISMAQTGNIHQFKVKTLEGEDFDFASLKGK
                     KIMIVNVASKCGLTPQYEDLQKLYDMYKDKNFVIIGFPANNFLNQEPGTAEEIMEFCS
                     RNYGVTFPMMEKISVKGKDMHPLYKWLTSKDLNGVLESDIQWNFQKYLIDENGNLVKM
                     IPPKQRILEQVIYDWIEGK"
     gene            complement(14444..16252)
                     /gene="typA"
                     /locus_tag="PKY63_08110"
     CDS             complement(14444..16252)
                     /gene="typA"
                     /locus_tag="PKY63_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPF00613.1"
                     /translation="MHDKVKIRNIAIIAHVDHGKTTLVDRLLHQVKLFRDNQDMGELI
                     LDSNDLERERGITILAKNVSVRYKGFKINIIDTPGHSDFGGEVERVLNMADGVLLVVD
                     AFEGPMPQTRFVLEKALALNLKPIVVINKVDKPNCNPDHAMEAVFDLMFSMGANSDQL
                     DFPTVYGSAKKGWMGPDWKNETEDVSYLLDLIIERIPAPIIPEGNLQMMITSLDYSTY
                     TGRIAVGRILRGSLKFSDEIAICKKDGSIIRSKIKELYIFEGLGKERIKTEVPAGEIC
                     AVFGPQEFEIGDTIADAENPEPLKPIHVDEPTISMLFTINNSPFYGKDGKFVTSRHVR
                     DRLYKETEKNLALRVEETASADSWLVFGRGVLHLSVLIETMRREGYELQVGQPKVIIK
                     EVDGEKCEPIEWMTVNVKSDFSGKVIDIVTQRKGDIVDIENRGDRTRLEFSIPARGLF
                     GLRNVVLTATEGEAVISHRFKGFEPWKGDFPVTRNGALIAMETGTAIAYSLNRLQDRG
                     SFFIEPFDNVYAGQVIGEQIRQDDLVVNVVVTKKLSNMRASGSDEKVMLAPPRRFSLE
                     EAMEYLADDEYLEVTPLALRLRKIILDHTERKRADK"
     gene            complement(16332..17606)
                     /locus_tag="PKY63_08115"
     CDS             complement(16332..17606)
                     /locus_tag="PKY63_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPF00614.1"
                     /translation="MKDFTAFADLFVWTSQQMALINIDWLPIEALTTIALLLVLSILA
                     LLFYFIGKFILFRIIRSIVKRTPGIYDDIFVSRKFLRRISYVIPMILLYQLIPFAIPH
                     YPGWVVTVRKLSEIVLIFFVLTSGYALLDSFYEKYLNTEKSRFKPVKGYLQVVKIVGG
                     FIASILVLSILLSETPGFLLGGLGAISAVLLLVFKDTILGFVASVQLSSNDMIMLGDW
                     ITVDKYKADGDVVEITLSSVKVKNFDNTFTYVPTYGMLTDSFQNWRGMQESPGRRIKR
                     SVNIHVSSVHFVTQEELEKYRKIELIHDYIDVKQKELAEYNKGVLCDSATIVNFRQQT
                     NIGIYRAYLLEYLKKLPFINHDEITMVRQLPPADNGIPVEVYAFTFEKEWAKYEAVMA
                     DIFDHILAATRYFDLEIFQSASGSDLRNIGHN"
     gene            complement(17606..18988)
                     /locus_tag="PKY63_08120"
     CDS             complement(17606..18988)
                     /locus_tag="PKY63_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPF00615.1"
                     /translation="MKHILFSLVLLLLPFLLSAQNISPEVLKKLQENYKASEPDKAAI
                     NALMSNDLKSLTVNAANCVKPDAYFKYRVKSAGITDQKSSGRCWLFASLNVYRPQVIS
                     KFNLADFEFSQNYLFFYDQLEKANLFLEGILETADKPLDDRRVEWLLKNAMGDGGVWG
                     GFANLVNKYGLVPAEIMPETKSSNDTRYMSSVLTTLLRQDALTLRAAFSAKKSMAELQ
                     LMKVKMLSDVYKVLAYNLGEPPTSFKYRFIDKNKVAGDYKEYTPLSFYQDAITFNASD
                     YIMFMDDPSREYYKLYEIQYDRNVQEGINWKYINIPAAEIKLMAIESIKNNEPMYFSC
                     DVGKQYDRNSSTLDTKNFDYESLYGIDFSMDKKQRIQTFESGSTHGMTLVGVDVDASE
                     KPVKWLIENSWGAVGFDGGHLIMTDEWFNEYMFRLVVNKKYVPAKIQEILKQKAIMLP
                     PWDPMFMEDE"
     gene            complement(19112..19294)
                     /locus_tag="PKY63_08125"
     CDS             complement(19112..19294)
                     /locus_tag="PKY63_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00616.1"
                     /translation="MKTKETTKKKTDKLNKLERALAEKQISYSEYEMWKTVYMNEKEF
                     VEEPRIVSRKKRRRRV"
BASE COUNT         5943 a         4503 c         3522 g         5567 t
ORIGIN      
        1 tccatcgaat agttggcacg gccgctggag aggtttcgca aagtagttgc ataaccaaac
       61 atttcggaaa gtggcacgaa tccattgagt ttttgcgaac ccttacgata acggcgcata
      121 gcttcaatac ggccacgtcg tttattgagg tcaccggtaa cagcaccaat ataatcatcc
      181 ggcgtattca cttcaagttt catgatgggc tcgagcagtt tcggagtggc tttcataaaa
      241 ccctgtttga aacacatagc agcacatagc tggaatgcca tatcgcttga atcgaccgga
      301 tgaaaaccac catcaaccag aacagctttg acatccacca ccgggaaacc agccagcgga
      361 cctttatcca gcgctttacg caatcctttt tcaaccgatg aaatatattc agcaggaata
      421 gctccgcctt tgattttatc aataaattca aaaccttttc cttcattcgg ctcgaaacga
      481 atgactgtat gcgcaaactg gcccttgcca cccgactgct tcacgtggcg ataattactc
      541 tcaacttcac cagtcaatgc ttcgcgatac gacacagccg gctctccaac ctcaacatcc
      601 actttaaatt cttctttgat ccggtccatg ataatttcaa gatgcaattc gcccatgcct
      661 gaaacgatgg tttcttcagt ttcctgatcg aaatgcgcgc ggaaagaagg gtcttcgtta
      721 cacaggcgtg ccaatgcttc gctgagttta tcgcggtctt tacggatagc cggatttatt
      781 ttcaggtcaa taacagcggg tggaacatga atgctttcaa gcattatttt attattcggg
      841 tcacagagtg tatcgccagt gcgggtaagt ttcaatccaa caagagccac aatttcgcca
      901 ggtcctgcct cggaaacttc aacacggtcc tttgcatgaa tgcgcaaaat tcgaccggca
      961 cgctcaggtt tacttttggt ggaattgtaa aaagccatac cacttgaaag cgttcccgaa
     1021 aaaattctca aaaaagtctg ctgaccaaca taaggatcat taataagttt aaatgccagc
     1081 gcagaaaacg gttctttcga cgaaggtcca cgggtaagca tcttttcgga attatcaaca
     1141 tcactaccaa caacagcgcc aatatcaaca ggcgatggaa gaaaatctat aaccgcatca
     1201 agcaataatt gaatgccttt atttttatag gcagcaccgc aaaataccgg tgtaatcaat
     1261 aattttattg tcgcttcacg ggcggctttc attaataatt catcagaaac ttcctgctca
     1321 ttcaaaaaca aatctccaat ggtttcatca taatctgcca aaacttcaac aatacgctga
     1381 cgaccagcca gagcggcttc tgtaaattcg gcaggaatat cgttttcaac acgctgatca
     1441 ccatcaaaaa aataagcgcg ctgatgaatc agatcaacca tacctgcaaa atcttcctca
     1501 acccccattg gaagctgaaa tggcaatgcc gatgcatcaa ggtatttatt catctgctca
     1561 acaactgcac taaaatcagc acctgttcga tccattttat tcacaaatgc aatgcgggga
     1621 actcgataac gatcggcctg attccatacg gtttcgcttt gtggttcgac accgccaacc
     1681 gcacaaaaaa cggctaccat tccatcaatt acacgtaatg aacgctctac ttcaatggta
     1741 aaatcaacat ggcccggagt gtctatcagg ttaatctgat gatccttcca gaaagcagtt
     1801 attgctgcag aagcaatggt aattccacgc tcctgttcct gcttcataaa atccatggtg
     1861 gctgcaccat cgtgcgtttc tccaactttt ctgttgacac ctgtaaaaaa caggattcgc
     1921 tcactgacag tagttttccc ggcatcgata tgcgctgcaa tgcctatatt cctcaatttt
     1981 gaaatgttac tcatctgaaa atgctgatta ttgttgattt ttatgctaat tgcctgtttg
     2041 ctgacaattt gagcgtgcaa aggtacgata aattatctga ctgaaaaagt ggtgtttgca
     2101 aaaaaatcga ccaaacctgt agatttaaga tttctgatca ggtgtccttc gtctctgctc
     2161 aggataaaaa tttttgattt tagattcttt cgttacaatc gttacaatcg tgaaatttat
     2221 tccgctgaaa gcgaaaatcg ttacaaggta acaagggtaa caccgcatcg agtattttcc
     2281 tttgagcact gcatgccgca tgcttttaca tcacctttaa aactttataa ccttataact
     2341 ttcaacttga gaagcaaagc gccgcaagct tcaaggaaaa tccagcgtta tcgcaggcgt
     2401 ttcaatcata ttgctttcat tcaatgcccg atcaagcata taggccttca ttttaatggt
     2461 gtcgctcacc aaaatcaatg aaagaatatt caaatcaatt tcaatactaa tgtcaccaac
     2521 cagatccttg tcaatatgtt ccggatttac atccggaata cgactgttat atggtgttgt
     2581 tatttcttga aaatcattgc cggttttctg aaaaaaagta atgaagaaat tgtaataaaa
     2641 attgctgccg ggattatatg gtggaagagt atctgactgc gcaagcccta catcgccgtc
     2701 tccatcagta aattcaatgg tcagatagcc aatgctgtca tgaccggcca cacttttcat
     2761 tgtcgcaaaa ctcttgaatg taatggctgg ttctatcgga tattcacgct gttcaataca
     2821 accgaaaaac gcaataccca aaattgctgt caggaagaat atttgtaact ttgaacgcac
     2881 tttcaaaaaa tttgcactaa ggtacgaaaa ataacgatga caggtcttga aatattatcc
     2941 aaatccattt tcagcattca gacttctgcg gaatttgaac atgccgcact cgctctcttt
     3001 tcctggcatt ataagcacaa tggcatttat agaatatttt gcaacaacac cgaaaagaca
     3061 ccggaaaatg tttgctcttt gcagcaaatt ccttgtctgc ctgtgaacct gcttcgcttt
     3121 aacaaaatac ttccggacgg tgtctccgct gaattgtttt tcgaaaccag cggtacaaca
     3181 gctgccgaaa ctggtcgcca ttatattgtt gatccaggtc tgtatctggc ctccgcaatg
     3241 aactgcttca gaaactttta tggcagtatc gaggattatg tcgtgcttgc attgcttccc
     3301 ggctatctcg aacggcagca ttcatcgctg gtgtatatgg ttgatcattg gataaaatgc
     3361 agcaataaac cggaatccgg attttacctg tatgatcacg aaaagctttt ttcaacgctg
     3421 aattcgttgc aaaataaaaa acaaaaaacg ctgctcattg gtgtcacaca cgcgttggtt
     3481 gatttttccg agaaatattc tctgggtttt ccgaaattga ttgtaatgga aaccggcggt
     3541 atgaaaggcc ggcgcaaaga actgtcgcga agtgaacttc atgcagtact gaaaaaatca
     3601 ttcggtgttc ccgccattca cagcgaatat ggcatgacgg aactgcttag tcaggcttac
     3661 tcaaaagcgg atggtatttt ccattgtccg ccctggatgc aggttttgct gcgcgatatc
     3721 aacgatccat tgtcacctcc gcaaaataaa aaaagtggag ccattaatat catcgacctt
     3781 gcaaacatgt attcatgccc ttttctggcc accgatgatg gcggagtcct gcatgatgac
     3841 gggtcatttg aaatcaccgg aagaattgat ttcagtgtaa tcaggggatg cagcactatg
     3901 atataaaata atgttagtac atttgcgtag agaaataagt taagtattaa tccatgaaat
     3961 ccggcttcaa taagaatttg attgtgtata tttggcttac tgttttgttt tccatgacgt
     4021 ctttttcaca gacctccgcg agcaaaatga tcgttgattt atatacgcaa gcttttgacc
     4081 aaattgactc aaccaatctg ccacaatatc gatacgttgt caggaaatat gattctgatc
     4141 gttattatat ctattccggg cgacagtcaa acattttgaa ctgctaccgg atggacattc
     4201 ccaaaacaga accagatacc caaagtccgg tttttcatat tcctattgac tctatttcag
     4261 atttaatcag gatcgatcaa aacagccaca ctgacttatg tcagaccgca gagcctcctt
     4321 acccttttaa aggtattaga aaaacacagt ttgacacgct ttgggaaaaa tggaatattg
     4381 atctgtacaa tattaatcag tacaagaatc ttgagagatt tttttccgca gacgctgcct
     4441 accataagct aatagatctt aaattgaaaa atcccgagtt gatatttgct tcaaaacccg
     4501 cagatgtttt attgggaatt gaccctgtca gtgccaacag atggaattac atatgtgatt
     4561 cacttaatgt gcacaatttt attcaataca aatcaaacct gtttgttttt gcattatatg
     4621 cgttcattat tcaatccaat ggtgaaattg tatatccacg tatttattat caattgcctg
     4681 aaaatccgct tgacctggac acagaaacag acaatttgaa aagcattatg caggtttccg
     4741 gcacaacggt aaaaaaagat tatgtggttc tgtattttaa atacggctat acgctcggta
     4801 caccgaattt ttattttcat ggggagaagt actgaattcc caaagaaaca tatgtaagct
     4861 aaataaatgc ctttttgaaa ctattggtaa aaagtctttt atcatttaat agttaagggt
     4921 atttttctaa ttttgcgcaa caaaacacaa ttccatgacc cccgaagaat tcaaaaaagc
     4981 aatcctggcc ggcattccgg cagaactacc tgctccgaaa ccgtataata ccaatgccaa
     5041 tcacgctccc aagcgtaaag acattcttac aaaaaaagaa aaacagttag ccatccgtaa
     5101 tgcgcttcgt tattttcccg aaagctggca cgccacgctg gctccggaat ttgccgaaga
     5161 gttgaaaaaa tatggccgca tttacatgta ccgcttcatg ccggaatatg aaatgtatgc
     5221 tcgcccgatt gaagaatatc ccgccaaatc acctcaggca gcagccatca tggttatgat
     5281 acaaaacaac ctcaatccgg cagtggccca gcatcccgaa gagctggtca cttatggcgg
     5341 caacggagca gtattccaaa actgggcgca atacctgctc accatgcagt atctggccaa
     5401 catgactgat gaacaaacac taaccatgta cagcggccat ccgatgggca tttatccaag
     5461 ccacaaagat gctccgcgtg tagttgttac caacggtctg gttattccaa actacagctc
     5521 acgcgacgac tacgaacgta tgaatgccat gggagtctct caatatggac aaatgactgc
     5581 aggatcattt atgtatatcg gtccacaggg aattgttcat ggaacaacca tcacagtact
     5641 aaacgcaggc cgcatgaaag ctgccaaagg agaaggtctg ggcggaatga ttttcgtcag
     5701 ctctggtctc ggcggcatgt cgggtgctca gccaaaagct gccaaaatcg ctggtgtagt
     5761 gggcattgta gccgaaataa atcctgctgc tgctaacaaa agatatgatc agggctgggt
     5821 tgatgaaatt ttctccgact tgcatgcact taccaaacgc gctgttgaag cggtaaaaaa
     5881 caaagaagcc ctttcaattg cctacgtcgg aaatattgtt gatctgtggg aacatctgta
     5941 taaagaaaat atttttattc acatcggatc agaccagaca tcgctccaca acccgtttgc
     6001 aggtggatac tacccggctg gcatgagctt cgaggaatcg aatatcatga tggcggagca
     6061 gcccgaaatg tttaaagaaa aagtatatga aagtctgcgg cgtcaggctg ctattatcaa
     6121 taaacatgcg gaacgcggaa cttatttctt cgattacggc aacgcatttc tgcttgaggc
     6181 ctcgcgtgcc ggtgccgaca tcatgaaaga agacggcact ttccgctacc caagctacgt
     6241 tcaggacatc atgggcccca tgtgtttcga tttcggcttc ggccctttcc gttgggtatg
     6301 tacttccggc aaaccagaag atctcgaaat ttctgacaac attgctgccg acgtacttga
     6361 aaaaatggct gccacttcgc ccaacgaaat cagtcagcag atgcgtgata acattcactg
     6421 gatccgcgaa gctcaaaaaa acaaactcgt tgtgggctcg caagcccgca ttctgtatgc
     6481 cgatgccgat ggccgcatta aaattgccga agcattcaac aacgccatca aagaaggaaa
     6541 aatttctgct ccgattgttc ttggtcgcga ccaccacgat gtatccggca ctgacagccc
     6601 gttccgggag acttcaaata tttacgacgg ttcaagtttt acagctgata tggctgttca
     6661 gaattttgtg ggtgacggat tccgcggcgc cacctggatt tcgttgcaca acggcggcgg
     6721 cgttggctgg ggcgaggtaa tcaacggcgg tttcggaatg gtacttgatg gaagtgctga
     6781 tgccgatcgc cgactaaaaa gcatgctgtt ctgggatgta aacaatggca tttcacgtcg
     6841 caactgggcc cgcaacccaa atgccatatt tgcaatcaaa cgcgctatgg aagccaatcc
     6901 tgaactctct gtcactctgc cgaatctcgt caatgatagc ctgctggaca aactggattt
     6961 ataaaaaaat ccgatagctt tcgtaaaatg cctgcagcca tgaaatacaa tggttgtctg
     7021 attaattgct gcaaaaacag caaacaatag tcattcaata gaataatggc ctattgaaca
     7081 ctattcgctc tttcagacat cactgacaaa cacattcatc aatataacgc taactgaaaa
     7141 aatacgaaat tttcatcagg tgagatttta cataaacaat ttcacacaat caaaacaaat
     7201 gagttttatc ggcagaattt aatcattaat aaagaattca gttatgcatc aatttaatca
     7261 gattgcatca gtagtgcccc tgataatagc cggcgcagcc attatcatac tattaatacg
     7321 ttatttttta ctcagacaga ttcatcattt ctttctgatg ctggtttttg ttttgctttc
     7381 attcagctac atctacatct ggcttttctg ccagcaggaa tacgaaacaa taacatggct
     7441 tttcccttta cgtcaacctg taaaaatcct gctgatacct gcctgctatt tttatatagc
     7501 tgcaacattt tttggcagaa agctgaactg cggaattact ctgttgcaca tacttccatc
     7561 cctttttatt tttcttgggt ttataccatt ttggtttgtg tcgcccgagg tgcaaataaa
     7621 ctatttatcc actgattacc aaaatattac acacaatcca atcattgaaa atgttcagat
     7681 tttaagattg ctaactgttt ttataatttt caatgctatg ctcatcgcat atacatggct
     7741 ggccattcac gaaatcaaaa aatacaaaaa aacaaacaat gtttcgctct tttctcttaa
     7801 aggcagcaac acattcagca ttattcttgt ttttctggga ttgttgcttg tattattcga
     7861 taatgcctat tttctgggga ttaccaaaac accagtttca gaaattgtgt tcagtattct
     7921 ttcgtctgta ttattaatgc tgatattcat acatggatac atgcaaaaag aatgcattac
     7981 atgtaaaaaa aacacaagaa aaaatacaaa taattttgca acaagcaaaa ccaatgactg
     8041 actaaatcag ctcctgctct atctcagcca tagcacccaa agccatcgtt gcaaaggtat
     8101 caatcggcaa tcctattatc tcgcattcga gaatattttc gcgcttcacc gatgctgcaa
     8161 aatgaacttc tttcatgcgc ttaacaaccg atttcggctt tacactgctg atttttttat
     8221 ccgggtaaac caatgctgtt gcagttatca aaccggtgat ggtttcnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnntgcc tgtcaagtaa ttcgagtgca gcagagcgtg
     8401 tcatgagatt tttttgcaaa tatacagaaa gctgtcattt tcagctttaa aaagtcatgc
     8461 atattgacaa cacgcaatca cctgaaattg tttaacttat aagaacaaaa aattattttc
     8521 cccaatcatt tggtcagttc aaataaaatt tctacttttg caacccaaaa ttcacggaaa
     8581 tggcaaagaa atcaaaagaa gcgcgcgttc aggttattct tgagtgcacc gagcacaaaa
     8641 acagcggtgt tcccggtact tcgcgctacg ttaccgttaa aaacaagaaa aatacgcccg
     8701 agcgtatgga gctgaagaaa tacaacccca ttctgaagaa agttactgtt cacaaagaaa
     8761 ttaaataagc tgagtcatgg caaagaaagt agttgcaacc ctgaaaaccg gccagacacg
     8821 tggatacacc aaggccatca aaatggtgaa atcggaaaaa accggcgcct attgcttcaa
     8881 acaaagaatt ctgcctgctg acgaagctca gacattcttt aagaagtaaa aaatattgtc
     8941 tttataaata aatgacttcg ggatgcaatt gtattccgaa gttatttttt atgctggtct
     9001 gaatctcttc agccagcatt tttatttcct gcccgctggc accacccaga ttgatcagaa
     9061 ttaatggctg cttatcgtga acaccagcat taaacccctg aaaacctttc cagccgctct
     9121 tttctatcag ccacgccgcc ggaattttat accgttcatc agcttcggta taaaaaggca
     9181 tttccggaaa ttttttatgc agctgctcaa ataacttttt ctccactacc ggattcctaa
     9241 aaaaactacc ggcattccca attagcttat aatcagggag tttactctcc cgaatggcaa
     9301 tgacagcatc gcgcacaacc atcggatcac ttccatcaag attgtttttc tggagataat
     9361 cctccactgc cttataatgc gtgctggttg agggcaccgt tttcagacgg taatccactg
     9421 aaacaaccat ccacgacggt ttagacttga agatgctgtg ccggtatccg aaatcacaat
     9481 ctttattctc tatatcaaaa aatttcaccg cttcgcgatc aaaaacatgg acgctgacaa
     9541 taaactgcga agcttcggca ccataggccc cgatattctg aactggcgta ctgcctaccg
     9601 ttccgggaat cagcgacaaa ttttccaatc caaaataacc ttgaatcagc gtttgctcga
     9661 ccagctcatc ccagatgcaa ccagcgtttg cacgaataat aacctcgtta tcattttggg
     9721 aaacaacttt ccaaccagca tagacaaagc gaatcaacgt gcctttaaaa tcgctcgtaa
     9781 aaagaagatt gcttccttga ccaatacaga aaaacggctc aggcaataaa gcatctgtca
     9841 aattcagcaa atcatctgca ttttcaattt cggccataca agcggctttc acatcgatgc
     9901 caaaagtgtt gaatgattta agagatatat ctctgattat tttcattttc gagagacttt
     9961 aaaaacaaaa accttgctgg cattttttct ggtaaaaaaa agaatcacat aatacacaaa
    10021 tgccgcaccc agccctatac ccgccgcctg caactcattc agtccatacg acaaaccacc
    10081 caaaacaccc gccatcaata atataaccgg cactacataa cccaatatgg cggcaagaat
    10141 tcccatccct tcagaaatag aaagtttaac atggtcacct gttttaaaac tgtgcggatc
    10201 ttcaaccggc acatcaaaca ctttttcttt catgtcggaa ggattgcaca cttttttcat
    10261 ctggcacgaa acacatgcgc ccacagtaag aatgcgaact ttaagaacat ttccatccac
    10321 agcatccact acgccatcgt gttcaatttc attcataatt ttcaattttt acttcgccat
    10381 tttttctttt acacctgcag caaacttaaa tttgaaactc taatcgatga atatcgctat
    10441 gaaaaagatc atttaaacaa aacaccaatc gaaatctgag ctattaaact gaaaggacca
    10501 cttgcaaatc gctgtatgcc aatcgctgtt tcaggcaatg aagcctgatc acgtatatgc
    10561 tcgtcgctgc tcataatcat tctggggcct ccgccaattc ctccgcaaaa tcccatcatt
    10621 acggcaggag caatcagttt gttataccca aaaacaaagt gcaatgttgg aataaaaaca
    10681 ggcactctgg tttttacagg atcatacaaa atattaaatc caacagtatc aacaggataa
    10741 tcaatgcttt gcgaatatga aacggcattt aatgtcagca ttagtccggc ataagcgccc
    10801 tgcatttttg gccttaccga taatccactg atttttacgg gctccgaaaa aattcgtttc
    10861 catgcaaaat tgacatgccc ccccctgagc ttttccttgc cggtcatcaa catgggtgca
    10921 ataataccag cctgcacctc catcgtattg cgcttctcaa acaaacgttc atatccgaca
    10981 taaaaatgat tgaacggaat accaaatgga ccagcttcaa tcatgttttt atacggcgaa
    11041 gctttcttca ttttggttac aatatccgga tacttctcaa aaaaataaat cgtttgcccg
    11101 ttttcataag ttagtcgctg aaattcacca gaatgaatcc tgtaggtttt atggggctca
    11161 tcaacaagaa tgacataaat gattttattt tcaattttca ctacctttcc aagaatttcc
    11221 tcatcatccc aggtcaccaa cgtatcctgg gctatcatca atgaagcatt attcaacaga
    11281 aaaaaagcaa aaaccagcca tttcatttct ttacaattta gctttgcgac gtgctttcaa
    11341 agtattcatc agcaaagcgg caatggttgt cagaccaact ccaccgggaa ccggtgtaat
    11401 ccaggatgct tttttagaca cttcaggaaa atcaacatcg ccagtcaaac ggaaaccact
    11461 ctgtttactt gcatcttcaa ttcggtgcat tccaacatca ataacaaccg cgccgggttt
    11521 gaccatatcg gcggtaacaa aattcggctt accgatggct acaattaaaa tatcagctgt
    11581 tgaagctatt tccttcaaat tctgagtctt gctgtgacaa agagtaaccg ttgaattgcc
    11641 tggattagaa gtgcgcgaca tgagtataga catcggagta ccgacaatat tgctgcggcc
    11701 aatcaccaca caatgttttc caacagtctc aatctgactt ctacgcaaca tttccatgat
    11761 accaaaagga gtggccgaaa taaaagtatc ctgtcccaat accattttac cgatattgac
    11821 aggatgaaaa ccatcaacat ctttttcagg attgatggcg ccaataacct tgttttcatc
    11881 aaaatactcc ggaagcggca actgaacaat aaatccatca acttcaggat ctttgttgag
    11941 aaattctatc acatccatca attgcttttc ggtcgttgtt tcatccatcc tgtaaatagt
    12001 cgaggtaatg cccaccgaag tgcagttttt ttccttactt gcgacatacg tctggctggc
    12061 cggatcttcc ccaaccagta ctgccgccag atgtggaggg cgttcgccgc gatcaatcat
    12121 agctgcaact tcttttttta tttcttcttt gatctgttcg ctgatcagct ttccatcaat
    12181 cagattcata tttttgtttt ttcggaaacg ttgttcgtta cacgttacag tcgttacacg
    12241 ttacatttag ctttcagttg tcagtaatca gcaatcagct ctctttagaa gctgaacaga
    12301 ttaaaccaac tcattttcaa attttcaaat tatcacatca gcacatcaat gtcttctcat
    12361 ctgcttcatc tgcttcatgg catttcgagc gccgcccgaa gtaaccatct tcatcatttt
    12421 gcgggtatcc tcaaattgtt tgaccagttt attcacctca gcaacagtag ttccggaacc
    12481 attggcaatg cgcattctac gtgatccgtt caataacgaa ggattggttc tttcttccgg
    12541 ggtcatcgaa taaataattg cttcaattcc cttgaaagca ttgtcatcaa tctccacatc
    12601 tttcatggct tttcctattc cgggaatcat gccaatcaaa tccttcatat tacccatctt
    12661 tttcacctga tgaatctgtt tcagaaaatc atcgaaagtg aactgatctt tggccagttt
    12721 cttctgcagt tttcgtgctt catcctcatc aaactgttcc tgggcttttt caacaagaga
    12781 tacgatatcc cccattccca gaattctgtc agccattcgt tgaggataga aaacatcaat
    12841 ggcatccatc ttttcaccga taccaacaaa tttaatcggc ttattgacta ccgccttgat
    12901 cgaaatggca gcaccaccgc gtgtatcacc atcgagtttt gtgagaatca caccgtcaaa
    12961 attcaacaca tcgttgaagg ccttggctgt attcaccgca tcctgcccgg tcatggcatc
    13021 cacaacaaac aacgtttctg cgggattaac cgcatcgtgg atattgcgga tctccttcat
    13081 catctcctca tccacagcca aacgaccagc agtatcaaca ataacggtgt ttattccata
    13141 ttgctttgca tgtgcaacgg cattctttgc aatacgaacc ggatctgtca ctccttcttc
    13201 ggtataaacc tgaacaccaa tctgctcagc caaaacctta atctgatcaa tggcagccgg
    13261 acggtacaca tcgcaggcca ccaacaaaat ctgtcgccct ttcttgcttt tcagatatga
    13321 agccagttta gccgagaatg ttgttttacc tgacccctgc aaacctgaca tcagaataac
    13381 cgaaggattg ggtttaagat tgatttccac tttgtcgcct cccatcaggg atacaagctc
    13441 atcgtggaca atcttaacca tcatctgacc aggagaaacc gctttgagaa catcctgccc
    13501 cagcgctttt tcttttaccg tatcggtaaa ctgtttggca gtggcgtaat ttacatcggc
    13561 atccaacaat gccttacgaa tctcctttac cgtttcggca acattgattt ctgtaatcct
    13621 gccctgccct ttcaacatct taaaagcgcg atccaaccta tcgctcagac cttcaaacat
    13681 agcttttcaa ttttgtgcaa aattagtcat ttattttcat tttttcaagc actctgaaat
    13741 gagcccttaa actgatgata attgtcatta aaccaagaaa agacaaacat tgtattttat
    13801 ctttgcatca tcaaaaaaaa tgaaaaaaat gaaaacaaca atcataatac tggcagcttt
    13861 cataagcacc ataagcatgg ctcaaaccgg aaacatccat cagttcaaag taaaaacact
    13921 tgaaggcgaa gattttgatt ttgcttcact gaaaggtaaa aaaataatga ttgtaaacgt
    13981 agccagtaaa tgcggactca ctccacaata cgaagatctt caaaaactgt atgatatgta
    14041 caaagacaaa aactttgtca ttattggatt tcctgccaac aattttttaa atcaggaacc
    14101 cggaacagca gaagagataa tggaattctg cagcaggaat tacggagtta cattcccaat
    14161 gatggaaaaa ataagcgtaa aagggaagga tatgcatcct ctgtataaat ggcttacaag
    14221 caaagatctg aacggcgttc tggaatccga tattcaatgg aactttcaga aatatctgat
    14281 agatgaaaac gggaatctgg taaaaatgat tccgcccaaa cagcggattc tcgaacaggt
    14341 tatttacgat tggattgaag gcaaataata aacctcatcc aggattttat tgaaaatcag
    14401 aataaataaa aaaggctgaa atggattcag ccttttttat ttattatttg tcagcacgct
    14461 tgcgttctgt atgatcgaga ataattttgc ggaggcgaag cgccagaggc gttacttcaa
    14521 gatactcgtc atcggccaga tattccatgg cttcttcgag cgaaaaacga cggggaggag
    14581 caagcattac tttctcatcg ctgcccgaag cgcgcatatt acttagcttt ttggtaacaa
    14641 caacatttac cacaagatcg tcctgccgga tttgttcacc aataacctga ccggcataca
    14701 cattgtcaaa aggttctata aagaatgaac cgcgatcctg caaacgatta agtgaataag
    14761 ctatagcagt acctgtttcc atggcaatca acgcgccatt gcgggttaca ggaaaatctc
    14821 ccttccaagg ttcaaatccc ttaaaccgat gtgatattac agcttcgcct tcggtagctg
    14881 tgagcacaac attacgcaaa ccgaacaacc cacgtgcagg tatcgaaaac tcaagccggg
    14941 tgcgatctcc acgattttca atatcaacaa tatctccttt ccgctgtgtg acaatatcga
    15001 ttacctttcc gctgaaatca gatttcacat tgacagtcat ccattcaatc ggttcgcatt
    15061 tttcaccatc aacttcctta atgataacct ttggctgtcc tacctgcaat tcatatccct
    15121 cgcgacgcat cgtctcaatc aatacactga gatgtaagac gcctcgtcca aaaacaagcc
    15181 aggaatcggc cgaagccgtt tcctcaacgc gaagtgccag atttttttcc gtttctttat
    15241 atagtctgtc gcgcacatga cgcgaggtta caaactttcc gtcttttccg taaaacggcg
    15301 aattgttgat ggtaaagagc atggaaatgg ttggttcatc aacatgaatg ggcttgagtg
    15361 gctcaggatt ttcagcatcg gcaatggtgt cgccaatttc aaactcctga ggcccaaaaa
    15421 cagcacatat ttcacctgcc ggaacctctg tctttatgcg ctctttgcca agaccttcaa
    15481 aaatatacaa ttccttgatt tttgatctga tgatactacc gtctttttta caaatggcaa
    15541 tttcatcact gaatttcaaa gaaccgcgca gaatgcgacc tacagcaata cgtccggtgt
    15601 aagttgaata atcaagtgaa gtaatcatca tctgcagatt tccttcagga ataatcggag
    15661 caggaatacg ttcaatgatc aaatcaagca gatagctcac gtcttcggtt tcgtttttcc
    15721 aatccggacc catccatcct ttttttgccg agccataaac agtcggaaaa tcaagctgat
    15781 cactgtttgc ccccatcgaa aacatgaggt caaaaacagc ttccattgca tgatcgggat
    15841 tacagttggg tttgtcaact ttattgataa caactattgg tttgagattc aaagcaagtg
    15901 ctttttccag cacaaagcgg gtttgaggca ttggtccttc gaaagcatct acaacaagca
    15961 aaacgccatc ggccatattg agcacgcgtt ccacttcgcc accaaaatcg gagtgacccg
    16021 gagtatcaat aatattaatt ttaaacccct tataacgaac tgaaacattt tttgccagaa
    16081 tggttatccc tctctcacgc tcaagatcat tactgtcgag aatcaactcg cccatgtcct
    16141 gattatcccg gaataatttt acctgatgta gcaatcggtc aaccagtgtg gtttttccgt
    16201 ggtcaacatg cgcaataatc gcaatatttc tgatttttac tttgtcgtgc atcctttttt
    16261 gaaataaggg cgcaaaggta cgattaattt tcgcagcaat aaagttaaat cagtaaatta
    16321 agaacttatg ttcaattatg gccaatattc ctcaaatcgg agcctgatgc agactggaaa
    16381 atctcaagat cgaaataacg tgttgccgcc agaatatgat cgaaaatatc agccatcact
    16441 gcttcgtatt tagcccattc cttttcgaaa gtaaatgcat acacttcaac cggaattccg
    16501 ttatcggcag ggggcaactg tctgaccata gtaatttcat catgattgat aaacggcaat
    16561 tttttcaggt attccagtaa atatgcccga tagatcccaa tattggtttg ctggcggaaa
    16621 tttacaatag ttgctgaatc acataaaaca cctttattgt attcagccag ctccttctgc
    16681 ttgacatcaa tgtaatcatg aatgagttcg atcttacggt atttttctaa ttcttcctgc
    16741 gtaacaaaat gaacactgct tacatgaata ttcaccgaac gtttaatccg tcggccaggg
    16801 ctttcctgca ttccacgcca gttttgaaaa gaatcggtca gcattccata cgtaggcaca
    16861 tacgtaaatg tattgtcaaa attcttgact ttgacgctcg acaacgtaat ctcaaccaca
    16921 tcgccatcgg ctttgtattt atcaacggtt atccaatcac ccagcataat catgtcgttt
    16981 gaagacaact gcacggaggc aacaaaacca agaatggtgt ctttaaaaac cagcaacagg
    17041 acggcagaaa tagcacccaa tccgcccagc aaaaaccccg gtgtctcact tagcaagatg
    17101 gataaaacaa gaatagatgc aataaaacca ccaacgattt tcactacctg caaatagccc
    17161 ttcacgggtt taaaacgcga cttctctgta ttgagatact tttcataaaa actatcgagt
    17221 agcgcataac ccgatgtcaa tacaaaaaat atcaaaacga tttcagataa ttttctaacc
    17281 gttaccaccc atcccggata atgcggaatg gcaaacggaa tcagctgata caatagtatc
    17341 attggtatta cataactgat tcgtcgaagg aatttgcggc tgacaaaaat atcatcataa
    17401 atgcctggag tgcgctttac aattgaccgg ataattctga agagaataaa cttgccgata
    17461 aaataaaaca acaaggcaag aatactcaaa accaacagca atgctatggt tgttaatgct
    17521 tcaatcggga gccagtcaat attaattaag gccatctgct gtgatgtcca tacaaataaa
    17581 tcagcaaatg cagtaaaatc tttcattatt catcctccat aaacatagga tcccaaggtg
    17641 gtaacatgat ggctttctgt ttcagaattt cctgaatttt tgcaggtaca tactttttat
    17701 taaccactag gcggaacata tattcattga accattcatc agtcataatc aaatgccccc
    17761 catcaaatcc aacggcaccc catgaattct cgatcagcca tttcacgggc ttttccgatg
    17821 catcgacatc aacaccaacc aaggtcattc catgtgtgct tccactttca aaagtctgaa
    17881 tccgttgttt tttgtccatc gaaaaatcaa tgccatacag actttcgtaa tcaaaattct
    17941 tcgtgtcaag cgtacttgaa ttccggtcat attgtttacc aacatcgcac gaaaaataca
    18001 tgggttcatt gttttttata ctttcgatgg ccattagctt gatctcagct gcaggaatat
    18061 tgatgtattt ccagttgata ccttcctgaa cattgcggtc atactgtatt tcatacaact
    18121 tgtaatactc acgcgaagga tcgtccatga acataatata atcagaagca ttgaatgtga
    18181 tagcatcctg atagaaagat aaaggagtat attccttgta atcaccggca actttattct
    18241 tgtcaataaa acgatatttg aacgatgtcg ggggttcgcc aagattatac gcaagcactt
    18301 tgtaaacatc ggaaagcatt ttcactttca tcagctgcag ttccgccatt gactttttgg
    18361 cgctgaaagc ggcgcgcaat gtcagtgcat cctgacgcag caaggtcgtc aaaaccgatg
    18421 acatgtagcg ggtatcgttg ctgctttttg tctcaggcat aatttcggca ggcaccaaac
    18481 catacttatt taccagattt gcaaatcctc cccaaacacc gccatcgccc atggcatttt
    18541 tcaacagcca ttccacacgg cgatcatcaa gcggtttatc agcagtttca agaatgcctt
    18601 caagaaaaag attggctttt tcaagctgat cgtagaaaaa aagataattc tgcgagaact
    18661 caaaatcagc cagattaaat ttactgatga cctgcggcct gtacacattc agactggcaa
    18721 aaagccaaca acggcctgaa ctcttctgat cggtaatacc agccgatttc actctgtact
    18781 tgaaatatgc atcgggtttt acgcaattgg cagcatttac tgtcaacgac ttcagatcat
    18841 tactcatcaa tgcattaata gccgccttat ccggttccga cgccttataa ttctcctgca
    18901 gtttttttaa aacttccggc gaaatattct gtgctgacaa caaaaatggc aacagcagca
    18961 aaacgagtga aaacaaaata tgtttcattg ttcaagtatt ttttgcgaaa ataaaaagaa
    19021 aatcatcatt acaaaaaaga atgacctgat tttgacaaat aaaaaccaga gtccatcatc
    19081 aaacagactc tggctaaaac ctattaaaaa cttaaacgcg tcttctcctt tttttgcgcg
    19141 aaacaatccg gggctcttca acaaattcct tttcgttcat atataccgtt ttccacattt
    19201 catattcact gtacgaaatc tgtttttcag ccagagcgcg ctcaagtttg tttaacttgt
    19261 ctgtcttctt ctttgttgtt tcttttgttt tcatgtaaac ctcttttcaa tttggggatt
    19321 caaatcctat aatgttaaat atcaaagcaa ttatcatgac aaaaatatat gtttttttat
    19381 atatccaatt ttctttatat gttttttaaa acttctaaaa cattcatatg cgtacaatgc
    19441 aatatcgatg ataaacaaaa acatagatgt atatacacat tcaacaaacc aacaaaagaa
    19501 ttttatcata ttaataattt ttgattcagt tgtcagcttt acagatacca agggttcatt
    19561 attaaatgca aaacccaaca ttaagtaact gtctaaattt tattttctaa ttctattaag
    19621 cataagctta atcat
//
LOCUS       DAOPKE010000054        19426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_2977_length_19426_cov_11.677817, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000054 DAOPKE010000000
VERSION     DAOPKE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2977_length_19426_cov_11.677817"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1375)
                     /locus_tag="PKY63_08130"
     CDS             complement(170..1375)
                     /locus_tag="PKY63_08130"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPF00617.1"
                     /translation="MEKKLNSIEEVLPDLKAGKVIIVVDDEDRENEGDFITAAETITS
                     ETVNFMARYGRGLICAPLTAERAASLKLDLMVKENTSLHETPFTVSVDLLRDGCTTGI
                     SASDRAKTIAAIANEKYLPEDFGRPGHIFPLRARSGGVLERAGHTEACVDMCKLAGMK
                     PVAALVEIMNEDGTMARLPQLYEIAEKFNLKITSVKALIEYRLKTECLVRMEEAVNLP
                     TEYGTFKLIAFTQLTNNLPHLALVKGDITTPEPVLIRVHSSCVTGDIFGSHKCDCGPQ
                     LHRSMQMVEKEGRGVVLYMNQEGRGIGLINKLKAYHLQELGRDTVEANLELGFKGDER
                     DYGIGAQILRMLGITKMKLITNNPVKRIGLESYGLEITETIPIIVEPNPYNQFYLETK
                     RNKMGHKLK"
     gene            complement(1570..>2016)
                     /locus_tag="PKY63_08135"
     CDS             complement(1570..>2016)
                     /locus_tag="PKY63_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF00618.1"
                     /translation="MPGLVETSNNLASVDTENGNIVIKNLLRSSVDSAKDALGHAVSG
                     VFELAGAEVELSGGYPGWKPNMDSAILKSCREMYQGMYGKLPEIKAIHAGLECGLLGA
                     VYPNWDMISFGPTIRYPHSPDEKVNIASVKKFFDFLVKVLENAPAK"
     assembly_gap    2017..2116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2117..2880)
                     /gene="pepD"
                     /locus_tag="PKY63_08140"
     CDS             complement(<2117..2880)
                     /gene="pepD"
                     /locus_tag="PKY63_08140"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPF00619.1"
                     /translation="MNHDIQNLEPKEVWTYFHALTQIPRPSKHEEKVREYMVNFGKSL
                     GLETIVDKVGNVIIRKPATPGMENRKGIVLQGHIDMVPQKNSDKEFDFEKDPIEAYID
                     GEWVTANGTTLGADNGMGVAAAMAVLASKTIKHGPIEALMTIDEETGMTGAFGLEAGV
                     LKADILINLDSEDEGEMYVGCAGGLDANITMKYTTIPVPAGHKAYKLSLTGLKGGHSG
                     MEIILGRGNANKIMNRFIKHAVEEHGMMVASIHGGSL"
     gene            3282..3956
                     /locus_tag="PKY63_08145"
     CDS             3282..3956
                     /locus_tag="PKY63_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPF00620.1"
                     /translation="MKIAENIQRITSQLSPQVRLVAVSKTKSVEDLREAYDAGQRIFG
                     ENYVQELCSKQPVLPADIDWHFIGHLQTNKVKFIAPFICCIQSVDSEKLLKEIDKEAH
                     KNNRVIDVLLEISIASDGSKFGFEPDEAFSFLGEGRYKIFQHVRFSGVMGMGSFSNDA
                     TITRSEYHLMKTVFDRLKANVFADCDSFCEISMGMSHDYGIAIEEGSTLVRVGTFIFG
                     ERNYNQ"
     gene            3958..5067
                     /locus_tag="PKY63_08150"
     CDS             3958..5067
                     /locus_tag="PKY63_08150"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HPF00621.1"
                     /translation="MKTFFVVVIVMLSAGCMAQTIYTSHVACGKIRYFSLVDVAEAVW
                     YQPDDSTGELHFYDQKRKQSCLVVVGINPYAEPVYTGSSDLFHEAKIDLTNNSVLRLI
                     IKEKQNSEPLILNLPATSSDKAVAVDYRCFSAVRYISYDTGEGTDSVSMTIEHLYPVP
                     AANTPAADSIRFVKAICSIYDALPVDAVYPDDMIKNFDIAYIQDYTSMYESGEAEVDE
                     VWANWDVYDEISILFQKNALIGFVSSGYEYTGGAHGLYGSVNYTYDLQSEKLISLEDL
                     FVMNFESILETAINEQLRSDLNLLPDALLSDEGFFVEVVTATPNFMLSEIGITFHYNI
                     YEIAPYAAGSIDITVPYDAIRSIIPAGGVIERMMK"
     gene            5089..5718
                     /locus_tag="PKY63_08155"
     CDS             5089..5718
                     /locus_tag="PKY63_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00622.1"
                     /translation="MVNCKIVFLIVAFAIAIPVFGQKHYTLLQTNTFQILPGNPNLHI
                     EKHLGGQVSVSLELMYTGRSWTNDGSIFFGGDFYRSNGFLIGLAPRLYFPLRKEVPNA
                     WYVSVLLRYSFAKFSDFTYYDYELYDDFDYRADITESENEIGMVIGRTFYLWKPVTAD
                     VFMGAGGGYGDRKRTFIEGHFENFRAYETYGLGKFYFGFKVGYCFGRKK"
     gene            5793..7397
                     /locus_tag="PKY63_08160"
     CDS             5793..7397
                     /locus_tag="PKY63_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HPF00623.1"
                     /translation="MNTIFKDSMSIPEEYRIGRIDQKEYLVNGEIRSWSGSCEEVLSP
                     VCFPNANGLSPAAIGSYPMLTEKESLEALESAVSAYDKGKGIWPTMSVAERIDCMKNF
                     IAEMKTKRSEVVNLLMWEIGKSLPDSEKEFDRTVEYIEGTIQSLKELDRVSSRFVYEQ
                     GIFAQIRRAPLGVVLCMGPYNYPLNETFTTLIPALIMGNTAILKPARYGILLLRPLLE
                     AFRNSFPAGVVNTIYGDGKTIITPLMQSGKIDVFAFIGSATVANIIAGNHPKPNRLKP
                     VYGLGAKNPAVILEDADIDLTVKECVSGALSFNGQRCTALKILFVHSKIAGVFLDKLS
                     KAVSALKSGLPWDPGVFLTPLPESGKIESMQAYVDDALEKGAKIINPHGAFHDATFFY
                     PAILYPVSNQMRVYHEEQFGPVIPVVPFDDISVPIRYIEDSDVGQQASIFGKDYELMA
                     KLIDPMVNQVCRVNINSQCQRGPDTFPFTGRKDSAEGTLSVSDALRVFSIRTLVAARD
                     SDLNKEILRKILQKRNSKFLSTDYLF"
     gene            complement(7411..8595)
                     /gene="hflX"
                     /locus_tag="PKY63_08165"
     CDS             complement(7411..8595)
                     /gene="hflX"
                     /locus_tag="PKY63_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPF00624.1"
                     /translation="MKSQGNITAKPPERAILVGLITYEQPEAKALEYLAELDFLARTS
                     EAIVVKHFTQRLEKPDGRTFVGKGKLDEIAHYVEEHEIDIIIFDDELTPTQLRNIEEV
                     VQCKILDRTNLILDIFAFRARTAYARTQVELAQYEYLLPRLTRMWTHLSKQRGGIGMK
                     GPGEKEIETDRRIIRKRISLLKEELKDIDKQKQTQRKQREKLIRVSLVGYTNVGKSTL
                     MNVLSKSEVFAENKLFATLDTTVRKVTYNFLAFLLSDTVGFIRKLPHHLVESFKSTLD
                     EIRESDLLLHIVDVSHPNYEEQMETVSRTLLEIGAGDKPVIIVFNKIDLYKHEENFLE
                     EETDKTPQQLLEESIVGRIQVPAIFISAKKGINIDKLKEFVFNQVKELHAGKYPGTVP
                     FY"
     assembly_gap    8695..8794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8820..9161)
                     /locus_tag="PKY63_08170"
     CDS             complement(8820..9161)
                     /locus_tag="PKY63_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00625.1"
                     /translation="MKPAKAFYPFAAWLIRLSMLLFTYVFFFETIRAFDYKSVDFYIA
                     SAFALFSVLVLVGGFLSKPAMTVISAFFLCGLSIYQLIISFSDKPDATSVVYMLGISA
                     MLVLFSVGNKK"
     gene            complement(9164..10156)
                     /gene="trpS"
                     /locus_tag="PKY63_08175"
     CDS             complement(9164..10156)
                     /gene="trpS"
                     /locus_tag="PKY63_08175"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPF00626.1"
                     /translation="MRILSGIRPTGNLHLGNYFGAVKNFVRLQEENECFFFIADYHSL
                     TTHPTPEHLNNNVKQVLAEFVACGLDPEKATLYIQSDVPETIELYLFLNMNAYLGELE
                     RVTSFKDKVRKHPNNVNAGLLTYPTLMAADILIHHADKVPVGKDQEQHLEMTRTFANR
                     FNRMYKTEYFKEPEAFNFGAELVKVPGLDGSGKMGKSEGEGNAIFLIDDEKTISKKIM
                     RAVTDSGPTEPNQTPPQAIQNLFSLMDIVTAEDTVKFFRESYANCSIRYGDLKKQLAA
                     DMNIFIEPIRERIQSVASDELFLSKVAATGREKARASASKTIQEVREIIGFKTL"
     gene            complement(10153..11937)
                     /gene="recJ"
                     /locus_tag="PKY63_08180"
     CDS             complement(10153..11937)
                     /gene="recJ"
                     /locus_tag="PKY63_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPF00627.1"
                     /translation="MLAKRWILKDQGNEELIRLLMEQVNVNRTLANLLVQRGIDSYDS
                     AKAFFRPNLENLHDPFLMKDMDKAVDRLILAIERKEKLLLYGDYDVDGTTAVATLYKF
                     LKRFSLTLDYYIPDRYTEGYGISFKGIDYASETGCTLIIAVDCGIKDQEKVLYASEKG
                     IDCIICDHHRPGDDIPDAVAVLDPKQKDCSYPFEELSGAGVGFKLMQGLAQKLGLPFD
                     ELKSLLDLVTLSIAADIVPVIGENRIISFYGLKMINSNPSAGIEAILRTAGVKRRPDS
                     YYKTDYSFTKKITISDLVFIVGPRVNAAGRIQSGKNSVDLLISENENEADRIATQIDN
                     LNIERKSLDSNATTEALTAISESLDDAGRRSTVLFNPAWQKGILGIVASRLTEHYYKP
                     TIVFTSANEEGIITGSARSIKDFDIYEAIETCSDLLIHWGGHTYAAGLSLKESDFEEF
                     RKRFDEFAQSKITDDMMIPVIEVDSYLDFNEVTKKFYQVLKQFAPFGPGNMNPVFQTN
                     RVYDNGFSKLVGSNHIKLMVTQSHYKGVGLPGIAFQLGDYFSEIESQKPFDIVYALEE
                     NEWNGNTTLQLNIKDIRIEKAKEETQEQ"
     gene            12013..12585
                     /locus_tag="PKY63_08185"
     CDS             12013..12585
                     /locus_tag="PKY63_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HPF00628.1"
                     /translation="MKLVFATNNAYKLTEARQILSDGVDVISLYDAGFDSDIEETGLT
                     LEENASLKARTVFEATGLNCFADDTGLEVDALDGRPGVFSARYAGSGHNFSANVDKLL
                     DELTNVENRKARFRTAISLILDGKEYLFEGIVNGEIMHERAGAGGFGYDPVFRPEQYQ
                     LSFAELSAEEKNTISHRGKALQKMVEFLRT"
     gene            13081..17436
                     /locus_tag="PKY63_08190"
     CDS             13081..17436
                     /locus_tag="PKY63_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00629.1"
                     /translation="MIKNFYSSNVALVFTMLILSVMGLRGQSVLTNSGGLSISGNTTI
                     TVLGSVENNSGAVVDELALTSSAVVSDDITNNGIIDGRGSITLNGDWYNNNTFTCHTG
                     IVNFSGVHQALSGSVSSTFYDLNLDGSGIKTQTIDQTTTNILDLNSVELATTSYTMYV
                     TNASAAAITRVNGFVSSTVGGALSRVTNSAASYLFPTGSSTGTVRYRPVALTPVSTAA
                     NTYEVRMANYTPTTEGFNVGTKEAIICEVNPQFFHLLNHTSGTDLTTVMVYYDNVADG
                     SWDALGNWLATPSVQWYEIAGSSTSAAAPFYVATATDQTLPAAERSVALTKHALTVDL
                     GSDVTVCAGSPVVLDAGNPGATYIWSNSATSQTISVSSSGLYAVTVTNPVNGCETVDA
                     VNITIHSLPTVAATADDPDFCIGGNTTITASGAGSYSWSGLGTNISYMVAPLVTTEYT
                     VTGMDGNGCTNSASVTVTVHPEPAIVATADYPSLCPGESTPITGSGGTSYLWDNGLGA
                     GLTHTVSPLVTTTYHVTGTDMYGCTNTAEVTVTLTTAPAVVASAADPSICVGETTTIS
                     GGGALNYIWDNGLGAGASFSVNPGITTTYQVTGSSATGCSATAEVTVTVNPNPTISAS
                     VTDATVCDGENTTISASGGDSYLWSNGLGTNASNIVNPSTTTTYTVTGSSAAGCSNLA
                     TVTVNVNALPVITALASDPTLCNGESTTISAGGGDTYIWDNSLGAGVSHTVNPATTTT
                     YEVTGVDGNGCSNIASVVVVVNDLPSITAGADYPDLCVGESATITGNGGVSYEWDNGL
                     GLGISHLISPLVTTTYQVTGTDINGCQNTASVLVNVFDLPTVVASADHLSVCSGGSVT
                     LTGSGAASFIWDQGLGAGSSFTISPSATTTYIVTGSTSSGCSNSASITITVNPLPSIT
                     ASASDAEICSGNNTTISVAGGNTYNWSNSLGSSSSYIVTPTASTTYSVTGIDINGCTG
                     TAEVVVTVNPLPVITAVLDEPVVCEGDSTNLSAGGGIDYLWDQGLGTGALHTVSPSTT
                     TVYAVTGTDLNGCSNTANVTLTINPVLDAAMNPAGPFCSNDAVETLTAVNGGGTWQGT
                     GISNPVTGEFNPALAGQGTHQIIYTTGGSCPDSDTMDIVVNEVISATINAAGPFCETG
                     SDEILTAATNGGVWSGTGITNTVNGTFSPVVAGVGSHEVVYTTSGICPDSDTVQIMVN
                     PQADASIVAAGPYCDNEAAVTMFAVEPGGIWSGTGFTNTSSGTWDPALAGAGSHNITY
                     AISGACGDTASATIVVNETPDVIVTCFGESCFGANDGFAFVDISGGTAPYFINWDNAE
                     TTDTIDMLPPGTYDVSVSDANGCTRTASGTVDVSSDSCYTPHVFIPNIFSPNGDANND
                     VYLVQGKGITNFTLRIYDRWGEKVFETTDLTSGWDGEFNGKPVEQGAYPYAVSLIFEG
                     ETTVREYNGYITIVR"
     gene            17450..18487
                     /locus_tag="PKY63_08195"
     CDS             17450..18487
                     /locus_tag="PKY63_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPF00630.1"
                     /translation="MKKQITFTFAIILISVFGIRAQDMHYSQYNEAPLLLNPALTGFY
                     DGNHRFVLNYKNQWKNLGAAYNNYDFSYDISFNKGNTTTGFLALGITAFNDVTGDVQF
                     TTSGAILNIAYQLYLNESNMLGAGIYGGYVQKSLSDENMMWVNQYDGATGFDGTIQSN
                     ETFQNMDFGFTDFGAGLNYSFSNNTRGLKSNEGFKFNVGGAVQHVTQPAYQFTGTADE
                     VVDMRINAHFRSVIQMGSSNLALVPSFYYAMQGTQKEMLPGLGFRYQMREESKYTGFI
                     KDAFMTLGGHFRTGDAAIGYLLVELNDFALGVSYDVNVSKLNKFTNSNGGFEFMIRYI
                     LKNDNSSRTLL"
     gene            18517..19395
                     /locus_tag="PKY63_08200"
     CDS             18517..19395
                     /locus_tag="PKY63_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00631.1"
                     /translation="MLLKISNLGKVVTAQLIFLVSAVYVQAQVGIATTSITPHASAML
                     EVRAANMGILIPNVALTGTTDAITVASPATSLFVYNTATVSDVTPGFYYWDGSKWVRF
                     LTGASSDDWKLAGNAGTTAGTHFLGTTDAVDMVFKTNAVEQMRILSGGNVGIGTAAPT
                     NKLHVDGLGTTTAIYGQYDANRNAYMASSSRGVSASMNTGAASGYGGYFSHTNSSSSG
                     YGIYAETNFTGTTNPSWTYGARIISNSTTQDQYGIYNTANHTGTTGYVRGFYNDVDIA
                     AANTSLIYGIRRVRFR"
BASE COUNT         5044 a         3919 c         4424 g         5839 t
ORIGIN      
        1 aaataaattt caaacaatct ctgaagcttt ttctggtatt attgcggatg atcaaaaaaa
       61 tcaatcagca tttagaaatt caaaaattcc aactatcttt ttgtaacatc ccagatcata
      121 aatccgacct caaatctaaa atcaaaaatc accgatcgga tttcgtaaat cacttgagtt
      181 tatgacccat tttattccgt ttggtttcca gataaaattg attgtaggga ttgggttcga
      241 cgataatcgg aatggtttca gtgatttcga ggccatagct ttcgaggccg atacgcttta
      301 ccggattgtt ggtaatgagt ttcattttgg tgataccaag catacgcaga atctgagcgc
      361 caatgccgta gtcgcgctca tcgcctttaa atcctaattc gagattggct tcaacagtgt
      421 cgcggccaag ttcctgtaaa tgataggcct tgagtttatt gatcaggcca attccccgtc
      481 cttcctgatt catgtataaa accacgccgc gtccttcttt ttcgaccatt tgcatggagc
      541 ggtgcagctg cggtccgcaa tcgcatttat gactaccgaa aatatctccg gttacacatg
      601 atgaatgtac tcggatgagc acgggttctg gcgttgttat gtcgcctttc accagcgcca
      661 gatgcggtag attgtttgtt agttgtgtaa atgcaatgag tttaaacgtg ccatattcgg
      721 taggcagatt cacagcttct tccatgcgta ccaggcattc ggttttgagg cgatattcaa
      781 tcaatgcttt taccgaagta attttcagat taaacttttc agcgatttcg tacagttgcg
      841 gaagtcgggc catggtgccg tcttcattca tgatttcaac cagtgctgca accggtttca
      901 tacctgccag cttacacatg tcaacgcatg cttcggtatg tccggcacgt tctaaaacgc
      961 ccccagagcg ggctctcaat gggaaaatat gtccgggccg cccgaaatct tcaggtaaat
     1021 atttttcatt ggcgatggcc gcaatggttt tggcccggtc cgatgctgaa attccagttg
     1081 tacagccgtc gcgcaataaa tcgaccgata cggtgaacgg tgtttcgtgc agtgaagtat
     1141 tttcttttac catcaggtcg agtttcaatg atgctgcgcg ttctgctgtt aatggtgcgc
     1201 aaatgagacc acggccgtag cgtgccatga aattcactgt ttcagatgtg atggtttcag
     1261 cagcagtgat aaaatcgcct tcgttttcac ggtcttcatc gtcaactact ataattactt
     1321 ttcctgcttt caggtcgggg agcacttctt ctatgctgtt taatttcttt tccatggtca
     1381 ttttttcgat gtgcaaaagt acaataattt taggcaatgg cgcggatttg caaatccgcg
     1441 ccattgcctt gtaataataa aacatggcac gatttgcaat ccgtgccatg ttttatttct
     1501 taacttaaaa gttcaaagtg tctttaggaa ctttcaactt tacgatcccg atagctatca
     1561 ggattccact tatttagcag gtgcattttc cagcactttc accaaaaagt cgaagaattt
     1621 tttcaccgaa gcgatattta ctttttcatc cgggctgtgt gggtagcgaa tagtcgggcc
     1681 aaatgaaatc atgtcccaat tgggatatac agcaccaaga agaccgcact caaggccggc
     1741 atggatggct ttgatttcag gtagttttcc gtacatgccc tgatacattt cacgacagct
     1801 tttcagaatc gcgctgtcca tgttgggttt ccaacctgga tatccaccgg aaagctcaac
     1861 ttcggcacca gctaattcaa atacacctga aactgcgtgt cctagtgcgt ctttagctga
     1921 atctacagag cttcgcagca gatttttaat aacaatgttt ccgttttcag tatccacgct
     1981 ggccaggttg ttcgatgttt ctacaagtcc gggcatnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnagcg agcccccatg aatcgaagcc accatcattc cgtgttcttc
     2161 aacagcgtgc ttgataaagc ggttcattat tttattggcg ttgccgcgac ccagaataat
     2221 ctccatgccg ctgtgtccgc ctttcagtcc tgtgagacta agtttgtatg ctttgtgtcc
     2281 tgcaggaacc ggaatagtgg tgtatttcat tgtaatgttg gcatcgagtc cgccggcgca
     2341 acctacgtac atttcgcctt catcttcgct gtcgaggttg atgagaatgt cggctttcag
     2401 cacaccagct tcgagtccga aagcgccggt cataccggtt tcttcgtcaa tggtcatcag
     2461 tgcttcaatt ggaccatgtt tgatggtttt tgaagccagt acagccatag cggcggcaac
     2521 acccattccg ttatcggcgc ccagtgtggt tccgttggct gtaacccatt caccatcgat
     2581 gtaagcttcg atcggatctt tttcaaaatc aaattccttg tcgctgtttt tctgcggaac
     2641 catgtcgata tgtccttgca gtacaatgcc tttgcggttt tccatgcccg gtgtggctgg
     2701 tttgcggata atgacattgc cgactttatc aacaatggtt tcaagtccga gcgatttgcc
     2761 gaaattcacc atgtattcgc gcactttttc ttcgtgcttc gaagggcgtg gaatctgagt
     2821 cagtgcatga aagtaggtcc atacttcttt tggttcgaga ttttggatgt cgtgattcat
     2881 tctatttagt tttaaagatt tttgctgacc caaagatagt gattttagaa tatcgggcgg
     2941 gcgttttttg atttttgatt tgtggtgctt cgtgtggttg cgcgtctaaa gccgcatgac
     3001 cgtacatttc aaaggtgctt cacgtaaaac gtgacaggtc gcgtctcctt tgtccctggg
     3061 gcttccagcc caaggctaat attattcgat cagcgtccgc cgatcgcctc aaaggaacca
     3121 actatggtat ttttggggat aaacgtttat tgctgcaaca tatccgcagc cgatccttgt
     3181 aacccgtgga cttaattttt gttttcaaca ggggctatga ctgattcaga tcttgcaata
     3241 attttttaat ctatcatgtt tcttattaca tttgcagtat tatgaaaata gcagaaaata
     3301 ttcagcgaat tacgtcgcag ttgtcaccgc aggtgcggct ggtggcagtg agcaaaacca
     3361 agtctgtaga ggatttgaga gaagcgtatg atgcaggtca gcgcattttc ggtgaaaatt
     3421 acgtgcagga actatgttca aaacagccag tattgccagc cgatattgat tggcatttta
     3481 ttggtcattt gcaaaccaat aaagtgaaat ttattgcacc ttttatatgt tgtattcaga
     3541 gcgtggacag cgaaaaacta ctgaaggaaa ttgataagga agcacacaaa aacaatcgtg
     3601 tgattgatgt actgcttgaa atttccattg cgtccgatgg ctccaaattt ggctttgagc
     3661 ccgatgaagc tttttcattt cttggcgaag gacgctataa aatatttcag catgtgcgtt
     3721 tcagcggcgt gatgggcatg ggaagtttta gcaatgacgc aacaatcacg cgatctgaat
     3781 atcatttaat gaagacggta ttcgaccgct taaaagcaaa tgttttcgct gactgcgatt
     3841 cgttttgtga aatcagtatg ggcatgagcc acgactatgg aatagctatt gaagagggta
     3901 gcacattggt acgtgtcggg acttttatat tcggggaaag aaattataac caataaaatg
     3961 aaaacattct ttgtcgttgt gattgttatg ctgtcagccg gttgcatggc tcagaccatt
     4021 tatacttcgc atgttgcctg tggcaaaata cgttattttt ctttggttga tgtggcggag
     4081 gcggtatggt atcagccaga cgacagcaca ggagaattgc atttttatga tcagaaacgc
     4141 aaacaaagct gccttgttgt ggttggaata aatccttacg ctgagccggt ttacacagga
     4201 tcatccgatt tgtttcatga agcaaaaatt gatctgacaa ataattccgt cttgcgatta
     4261 ataatcaagg aaaaacaaaa ttcggaacca ttgatattga accttccggc aacatcttcc
     4321 gataaggcgg tggctgttga ttatcgttgt ttcagcgcag tcagatatat atcttacgac
     4381 acaggagaag ggactgattc agtttcaatg accattgagc atttgtatcc tgttcctgca
     4441 gcaaatacgc ctgcggcaga ttctattcgc tttgtaaaag ctatttgttc catctatgat
     4501 gctctgcctg ttgatgctgt ttatcctgat gatatgataa agaattttga tattgcctat
     4561 attcaggatt acacatcgat gtatgagagt ggtgaagccg aagttgacga agtctgggca
     4621 aattgggacg tgtatgatga aatttcgata ctgtttcaga aaaatgcttt gattggtttt
     4681 gtttcttcag gatatgaata caccggcgga gcgcatggac tctatggatc ggtgaattac
     4741 acctatgatt tgcaaagcga aaaactgatt tctttggagg atctttttgt aatgaatttc
     4801 gaatcaatat tggaaacggc cattaatgaa cagttgcgct ctgatttgaa tctgttacct
     4861 gatgcactgt tgagcgatga gggatttttt gttgaagttg ttactgcaac accaaatttc
     4921 atgttatccg aaatcggaat cacgtttcat tacaacattt acgaaatcgc tccatatgca
     4981 gccggtagca ttgatatcac cgtgccttat gatgcaattc gctccattat tcctgctggt
     5041 ggagtgattg agaggatgat gaaatagttt taatcaaact atacaggtat ggtcaattgt
     5101 aaaattgttt ttctgattgt tgcatttgcc attgccattc cggtttttgg ccagaagcac
     5161 tacaccttgt tgcagaccaa tacctttcaa attcttccgg gcaacccgaa tctacatatc
     5221 gaaaaacatc tgggcggtca ggtttctgtg tcgctggaat tgatgtacac aggccgctcc
     5281 tggacaaacg atggatcaat tttttttgga ggcgattttt atcgttccaa tggattttta
     5341 attggcctgg ctccgcgttt gtattttccc ttgcgtaaag aagttccgaa tgcgtggtat
     5401 gtgtctgtgt tgctccgtta ttcgtttgca aaattctccg attttactta ttacgattat
     5461 gaattgtatg atgactttga ttacagagcc gatattactg aaagcgagaa cgaaatcgga
     5521 atggtaatcg gacgaacttt ctatttatgg aagccagtaa cagccgatgt tttcatgggt
     5581 gccggcggtg gttatggcga tcggaaacga acatttattg aaggtcattt tgagaacttc
     5641 agagcatatg aaacttatgg tttgggcaaa ttttattttg gatttaaagt agggtattgc
     5701 tttggaagga agaaatgatt ttcgtctaat tcatttttac gatttcaaaa agcgtttttg
     5761 aatcattatc tttgtcatga aattttttta caatgaatac aatttttaag gacagcatgt
     5821 ctattccgga agaataccgc atcggcagaa ttgaccagaa ggaatatctt gttaatggtg
     5881 aaatacgctc ctggagcgga tcctgcgaag aagtgctgtc gcctgtctgc tttccaaatg
     5941 caaatgggtt atcgccggct gctattggca gttacccgat gctgacagaa aaagaatcgc
     6001 ttgaagcact cgaaagcgct gtatctgcct acgataaagg caagggtatc tggcccacaa
     6061 tgtcggttgc ggaaagaatt gactgtatga aaaatttcat tgcagaaatg aaaacaaagc
     6121 gcagcgaagt ggtaaatctg ctgatgtggg aaattggcaa aagtttgccg gattcagaaa
     6181 aggaatttga tagaaccgtt gaatacattg agggtaccat tcaatcgctc aaagaactcg
     6241 atcgggtgtc ttcgcgcttt gtttacgagc agggtatttt cgcgcagata cgtcgtgcgc
     6301 cgctgggggt tgtactttgc atgggaccat acaattatcc gcttaatgaa acttttacaa
     6361 cattgattcc tgcgctgatc atggggaata cagccattct taagccggcc agatatggca
     6421 ttttgctgtt acgccccttg cttgaagctt tccggaattc gtttccggcc ggcgtggtga
     6481 ataccattta cggagatgga aaaaccatca ttacgccgtt gatgcaatca ggaaaaattg
     6541 atgtatttgc atttattggt tcggccacag ttgccaatat aattgccggc aatcatccaa
     6601 aaccaaaccg gctgaaaccg gtgtatggcc ttggtgcaaa gaatccggcc gtgattcttg
     6661 aagacgcaga tattgatctg actgtgaaag aatgtgtgtc gggggcattg tcatttaatg
     6721 gacaacgttg tactgcactg aagattttat ttgttcacag caagattgcc ggtgtatttc
     6781 tcgataaact atccaaagcg gtatcggcat taaaatctgg attaccctgg gaccccggtg
     6841 tatttctgac tccgctgccc gaatccggaa agattgaatc gatgcaggca tatgttgatg
     6901 atgcgcttga gaaaggtgca aaaatcatca atccacatgg agcttttcat gacgcaacat
     6961 ttttctatcc ggccatactg tatccagtta gcaaccaaat gcgcgtttat cacgaagagc
     7021 agttcgggcc tgtgattccc gtggttccat tcgatgacat ttctgttccg attcgttata
     7081 tcgaagattc ggatgtcgga cagcaggcca gtattttcgg aaaagattat gaactgatgg
     7141 caaaactgat tgatccgatg gtgaatcagg tttgccgagt caatatcaac agtcagtgtc
     7201 agcgcggacc cgacacattt ccattcaccg gcagaaaaga ttcggccgaa gggacgctgt
     7261 cggtgagcga tgctttgcgt gttttttcaa tccgtacatt ggtggcagcg cgcgacagcg
     7321 atttgaacaa agaaatactg aggaagattt tacagaaacg caattcgaaa tttctttcga
     7381 cagattattt gttttagaac tggctgcaaa tcaataaaac ggtacagtgc ccggatattt
     7441 gccggcgtgc agttctttta cctgattaaa aacgaactct ttcaatttat cgatgttgat
     7501 tccttttttt gcggaaataa aaatcgcagg tacttgaatg cggcctacaa tactctcttc
     7561 gagcagttgt tgcggcgttt tgtcggtttc ttcttcaaga aaattttctt cgtgtttgta
     7621 taaatcaatt ttattgaaga caataatcac cggcttatcg cctgcaccaa tttcaagcaa
     7681 ggttctgctc acggtttcca tttgctcttc gtagttagga tgcgatacat cgacaatgtg
     7741 cagcaacaaa tcgctttcac ggatttcatc cagcgtcgat ttgaatgact caaccagatg
     7801 gtgcggcaat ttgcggataa atccaaccgt gtcgctgagc agaaatgcga gaaaattata
     7861 cgtaactttt cgaacggttg tatcgagcgt ggcaaataat ttattttcgg caaacacttc
     7921 tgatttcgaa agtacattca tcagcgtgct tttaccaacg ttggtataac caaccagcga
     7981 cacgcggatc agcttctcgc gctgttttcg ttgtgtctgt ttttgtttgt cgatatcttt
     8041 cagctcttct ttcagcaacg aaatgcgctt acgaatgatt cggcggtcgg tttcaatttc
     8101 tttttcaccg gggcctttca tcccaatgcc accgcgctgt ttcgaaaggt gtgtccacat
     8161 gcgtgtaagg cggggcagca gatattcgta ttgtgcaagt tcaacctgag tacgtgcata
     8221 agcagttctg gcgcgaaatg caaagatgtc gagaatcaga ttggtacgat caagaatttt
     8281 acactgaact acctcttcga tgtttcgaag ttgtgtaggt gtgagttcgt catcgaaaat
     8341 gatgatatca atttcatgtt cttcgacgta atgtgcaatt tcatcaagct tgcctttgcc
     8401 tacaaacgtg cgtccatcgg gcttttcgag gcgttgggtg aaatgtttta caacaatagc
     8461 ttcggaagtg cgcgccagaa aatcgagctc agctaagtat tcaagcgctt ttgcttcggg
     8521 ttgctcatat gtgatgagtc cgaccaggat ggcgcgttca gggggtttgg cggttatgtt
     8581 gccttgtgat ttcaatgcgt agctattttg attttagatg gcaaaagtac gcattttcgt
     8641 tatataggtg gtatgacctg atgctttgca tgttaaacca cttcgtggtt ttagnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncacttc gtggttttag gggatttttt
     8821 tattttttat tacccacaga aaacaaaacc aacattgcag aaatgcccag catatataca
     8881 accgaggttg catcgggctt gtcgctgaag ctgatgatga gttgatagat ggaaagtcca
     8941 cacagaaaga aggcgctaat tacagtcatg gctggttttg agagaaagcc accgacaagc
     9001 accagcaccg aaaacaatgc aaaggccgat gcaatataga agtcaacact tttgtaatca
     9061 aatgcacgta ttgtttcaaa aaagaacaca tacgtgaaaa gcagcataga gagccggata
     9121 agccatgctg caaacggata aaacgccttt gccggtttca taattataaa gttttaaagc
     9181 caataatttc gcgaacttcc tgaattgttt ttgatgcgct cgctcttgct ttttcgcggc
     9241 ctgttgctgc tactttcgac agaaaaagtt catcggaagc tacactctga atgcgttcac
     9301 gaataggttc aataaagata ttcatatccg ctgccagttg ttttttcaga tcaccatagc
     9361 ggatagaaca gttggcatac gattcgcgga aaaatttcac agtgtcttca gctgtaacaa
     9421 tatccatgag cgaaaacaaa ttctgaatgg cctgtggcgg tgtttgattg ggctcagttg
     9481 gtccactgtc ggttacagcc cgcataattt ttttcgaaat ggttttttcg tcatcaatca
     9541 gaaaaatggc attgccttcg ccttccgatt ttcccatttt gccgctgcca tccagcccgg
     9601 gaacctttac taattcagcg ccaaaattaa atgcttcggg ctccttgaaa tattcggtct
     9661 tatacatgcg gttgaaacga ttggcaaaag tgcgggtcat ttcaagatgt tgttcctgat
     9721 cttttccgac gggaaccttg tcggcatgat gaatcagaat atcagctgcc atgagggttg
     9781 ggtaggtgag taaccctgca ttgacgttgt tgggatgctt tcgtactttg tccttgaacg
     9841 atgttacacg ttcgagttca cccagatagg cattcatatt cagaaacaaa tacaattcga
     9901 tggtttcagg aacgtcgctc tgaatgtaaa gtgtagcttt ttccggatca agaccgcagg
     9961 cgacaaattc tgccaatacc tgtttgacat tgttgttcag atgttcaggt gttggatggg
    10021 ttgtcagact atgatagtct gcgatgaaaa aaaagcattc attttcttcc tgcaggcgca
    10081 caaaattttt gactgcgccg aaatagttgc ccagatgcag gttcccggtg ggccttattc
    10141 cgcttaatat tctcattgtt cctgcgtttc ttctttggct ttttcgattc ggatatcctt
    10201 gatgttcaat tgaagggttg tgtttccgtt ccattcattt tcttcaagtg cgtaaacgat
    10261 atcgaatggt ttttgtgatt ctatttcact gaaataatct ccgagctgaa aagcaattcc
    10321 tggcagtccg acacctttgt aatgactttg tgttaccatt agttttatgt gattgctgcc
    10381 gaccagtttc gaaaaaccgt tatcatacac cctgttggtt tgaaaaacgg ggttcatgtt
    10441 tccgggtccg aatggtgcaa attgcttcag tacctgataa aatttcttgg tgacttcatt
    10501 aaagtcgagg taagagtcaa cttcaattac aggaatcatc atatcatcgg tgattttcga
    10561 ttgggcaaat tcatcaaagc gtttgcggaa ttcttcgaaa tcagattctt tgagtgataa
    10621 acctgcggca taggtatgtc ctccccagtg aataagcaga tcgctgcagg tttcaatggc
    10681 ttcgtatatg tcgaagtctt tgattgagcg cgcgcttccg gtgatgatgc cttcttcgtt
    10741 tgcactggtg aaaacgatgg tgggtttgta ataatgctcg gttaaacgcg aagcaacgat
    10801 gccgagaatt cctttttgcc atgcgggatt gaaaagaacc gttgagcgtc gtccggcatc
    10861 atcgagtgat tcggaaatag ccgttaaagc ttcagtggtt gcatttgaat cgagactttt
    10921 tctttcgatg ttcaggttgt caatctgtgt tgcaatgcga tcggcttcgt tttcattttc
    10981 gctgatgagt aaatcaactg aatttttccc gctttgaatg cggcctgcag catttacgcg
    11041 tggtccaaca atgaaaacca gatcggaaat ggttattttt tttgtgaaag aataatcggt
    11101 tttgtaatag ctgtctggtc tgcgtttgac acctgctgtg cgtaaaatgg cttctattcc
    11161 tgcactcgga tttgaattga tcattttcaa tccataaaag gaaatgattc tgttttctcc
    11221 gataacagga acaatatcag ccgctatact cagtgttaca aggtcgagta aggatttgag
    11281 ttcatcgaat ggaagaccga gtttttgtgc aagaccctgc atcagtttaa aaccaactcc
    11341 ggcgcctgaa agttcttcga aaggatacga acagtctttt tgcttgggat ccagaacagc
    11401 cacggcatca ggtatatcgt cgcccggccg gtggtggtcg catatgatgc aatcaattcc
    11461 cttttcactg gcatagagga ctttctcctg atccttgatg ccgcagtcca ccgcaataat
    11521 aagcgtgcat ccggtttcgg aggcataatc aattccttta aatgaaattc cgtacccctc
    11581 tgtatatcgg tctggtatgt aataatccag tgtcagactg aagcgtttga gaaatttgta
    11641 caaggtggca actgccgttg taccgtcaac atcataatcg ccataaagca ataatttttc
    11701 ttttcgttcg atggcaagta ttagtctgtc aaccgcttta tccatgtctt tcatcaggaa
    11761 aggatcatgt agattttcga gattgggccg aaaaaatgct ttggcagaat catatgaatc
    11821 aatgccgcgt tgaaccagca gatttgccag ggtgcgattc acatttacct gttccatcaa
    11881 caggcgtatg agttcctcat ttccctgatc ctttaatatc caccgttttg ctagcataat
    11941 tctgaatgat gtgcaaatgt aagatatttt cgctgacttt gaaaagatta tttttgcatc
    12001 ttaaatcaga ttatgaaact tgtttttgcc accaataatg cttataaact caccgaagca
    12061 cgacagattt tgtcggatgg tgttgatgta atcagtttat atgatgccgg ctttgattct
    12121 gatatcgaag aaaccggcct gacactggaa gagaatgctt ctcttaaagc cagaactgtt
    12181 tttgaagcaa ccggtttaaa ttgttttgcc gatgataccg gccttgaagt ggatgcattg
    12241 gacggaagac ccggtgtgtt ttcggcgcgt tatgccggtt ctggacacaa tttcagcgcc
    12301 aatgtggata agctcctcga tgaattgaca aacgttgaaa atcgtaaagc ccgcttcaga
    12361 acagcaatca gtctgattct cgatggaaag gaatatctgt ttgaaggaat cgttaatggc
    12421 gaaatcatgc acgagcgcgc cggcgccggt ggctttggct atgatccggt tttcagacct
    12481 gaacaatatc agttgagttt cgctgaactg agtgcagaag aaaaaaacac catcagccac
    12541 cgtggcaagg ctttacaaaa aatggtcgaa tttctccgca cttgattgtt tattgcctgc
    12601 gcacctctgt tttaatttca ttgtgcttga tggccttttc tgaaatgcat gtttttgcat
    12661 aagttaattt gcggttaatt attatcactg cttacttctc gctagtttgt taattaaact
    12721 attgcgtttg attcatatca aaacagggcg cttaattcat cattaccgca atatggaaaa
    12781 aaatattttt caataaacat tcactgtgaa tatcgataaa atatcaaata ctacacatga
    12841 aatcatgatg actttcttta ttttctctgg ctcataagtt ggatcaatag tgtctgaatt
    12901 gtttattatc agtcatttaa gcctaatgtt tcaggccatt tcaaattttt atcaaattat
    12961 ttaaaaagaa taaatatgta ttttagggag tttatcgagt gtattacatc agaaaaattt
    13021 ttaattttgt gggctttttg ttggatttta atttttgttt ttacgtaaaa ctacatattc
    13081 atgataaaga atttttactc atcaaatgtt gcattggttt ttacaatgct gattctttca
    13141 gtaatggggt taagggggca atcggttctg acaaattcgg gcggattgag tatttcgggt
    13201 aatacgacca ttacagtgtt gggaagtgtt gaaaataatt caggtgcagt tgtggacgaa
    13261 ttggcgctga catcatcagc cgttgtttca gatgatatta ccaacaatgg cattattgac
    13321 ggccgtggct ccataacgct gaatggtgat tggtacaaca ataatacttt tacctgccat
    13381 acaggtattg tgaatttcag tggagttcat caagcacttt cgggttctgt ttcaagtaca
    13441 ttctatgatc tgaatctcga tggcagtgga ataaaaactc aaacgattga tcagactaca
    13501 acgaatattc tggatttgaa cagtgttgaa ttggcaacga cttcatacac gatgtatgta
    13561 accaatgcca gtgctgcagc tattacaaga gtaaacgggt ttgtaagcag tactgttggc
    13621 ggggccttga gccgtgtgac aaattcagcg gcttcgtatt tgtttccgac tggttcttca
    13681 accggaacag ttcgttacag acctgttgcc ttgacgcctg tttctactgc tgcaaatact
    13741 tacgaagtgc gtatggccaa ttatacacca acaaccgagg ggtttaacgt tggcaccaaa
    13801 gaggccatca tctgtgaagt gaatcctcag ttttttcatt tattgaatca tacctctggg
    13861 acagatctta caacggtaat ggtctattat gacaacgtag ctgatggcag ctgggacgcc
    13921 ttgggcaatt ggcttgctac gccttctgtg caatggtacg aaattgctgg atcaagcact
    13981 tccgcagcag ctccttttta tgttgctact gcaacagatc agacccttcc ggcagctgag
    14041 cggtctgttg ctttgaccaa gcatgccctg actgttgatc ttggaagcga tgtaaccgtt
    14101 tgtgcaggaa gtcctgttgt tcttgatgcc ggaaatcccg gggctactta tatttggtcg
    14161 aattctgcca ccagtcagac tatttcggtc agttcttcag gcttatatgc tgttacagtt
    14221 accaatccgg tcaatggatg tgaaactgtt gatgctgtca atattacaat tcatagtttg
    14281 ccaacagttg ccgctacagc tgatgatccg gatttctgca tcggcggcaa cacgacgatt
    14341 actgccagcg gtgctggttc atatagttgg agcggacttg gaaccaacat ttcatatatg
    14401 gtagctcctt tggttacaac agaatatact gtaacaggaa tggatggcaa tggttgtaca
    14461 aattcagctt ctgtgacagt taccgttcat cctgaaccag cgattgtagc cacagcagat
    14521 tatccaagtt tatgtcctgg agaaagcaca ccaatcacgg gaagcggtgg cacctcttat
    14581 ctttgggata acggtctggg tgcagggtta acacatacgg tttcaccatt ggtaacaaca
    14641 acttatcatg taacaggaac ggacatgtat ggatgtacca atactgcgga agtaacggta
    14701 acactaacca cggcgccggc tgtggttgcc agcgcagccg atccgtccat ctgtgtcgga
    14761 gaaacaacaa ccatcagtgg tggtggagct ctgaattata tatgggataa cggacttggc
    14821 gccggtgctt cgtttagtgt taatccgggt ataactacaa cttatcaggt aaccggatcc
    14881 agcgccacag gatgttcagc cacagccgaa gttactgtta ctgtaaatcc caacccgacg
    14941 atttctgcat cagttacaga tgccaccgtt tgtgatggcg aaaataccac catttccgca
    15001 tcgggaggcg attcctattt atggagcaat ggtcttggaa caaatgcttc caatattgtc
    15061 aatccttcaa caacaacgac atatacggta acaggtagtt ctgcagccgg ttgttcgaat
    15121 cttgccactg ttaccgttaa tgtgaatgca ttgcctgtaa tcactgccct ggcatcggat
    15181 ccgacattgt gtaatggcga gtccactacg attagtgccg gaggcggaga tacatacatc
    15241 tgggataatt cattaggtgc aggtgtctct catacggtca atcctgcaac caccaccacc
    15301 tatgaagtta ctggagttga tggtaacggt tgttccaata ttgccagtgt tgttgtggtg
    15361 gttaatgatt taccatcgat aacggctggt gcagattatc ctgatttgtg tgttggagaa
    15421 agtgcgacca ttacgggtaa cggaggtgtt tcatatgagt gggataatgg tttgggtttg
    15481 ggaatttcgc atttgatttc acctctggta actacgacct atcaggttac cggcaccgac
    15541 attaacggct gtcagaatac agcttctgtt cttgtcaatg tttttgattt gccaacagtt
    15601 gttgcatccg ctgatcattt atcggtttgc agtgggggtt ctgtaaccct tactggtagc
    15661 ggggctgcaa gttttatatg ggatcaggga ttgggagcgg gcagcagctt tacgatcagt
    15721 ccatccgcaa ccacaaccta tattgttaca ggttcaacat catctggttg ttcaaattcc
    15781 gcaagcatta ccatcactgt aaatccgttg ccctcaatta cagcatctgc ttcggatgca
    15841 gaaatatgtt ctggcaataa tacaacgatt tcggttgctg gcggaaatac ctataattgg
    15901 agtaacagtc tgggcagcag ttcatcatat attgttactc caacagcaag tactacctat
    15961 tctgttaccg gaattgatat taatggttgt accggtacgg ccgaagtagt ggttaccgta
    16021 aatccattgc cagttatcac agcagtattg gatgaaccgg ttgtatgcga aggtgactca
    16081 acgaatttgt cagctggcgg aggaatcgat tatctttggg atcagggatt gggaaccgga
    16141 gctttacata cggtgagccc ttcaactacg acagtgtatg ctgtaacagg gaccgatctg
    16201 aatggttgtt ccaataccgc caatgtaaca ctcaccatta atccggtgtt ggatgccgcc
    16261 atgaatccgg cgggtccatt ctgctccaat gatgcggttg aaacgctgac agctgtaaat
    16321 ggtggtggta cctggcaagg tacaggtatt tcaaatccag ttacagggga gtttaatccg
    16381 gctttggcag ggcagggcac acatcagatc atttacacaa ccggaggatc atgccctgat
    16441 tcggatacga tggacattgt ggttaatgaa gtcatttctg caacaattaa tgctgccgga
    16501 cctttctgtg aaactggcag tgatgaaata ttgacagcag caacaaacgg gggtgtctgg
    16561 agcggtaccg gtattaccaa tactgtgaac ggaacttttt cgcccgttgt tgcaggtgtt
    16621 ggcagccacg aagttgtata taccacctcg ggaatctgtc ctgattcaga tacggttcag
    16681 attatggtca atccgcaggc agacgcaagt attgtagctg caggtccata ttgcgacaac
    16741 gaagctgcag taacgatgtt tgcagttgag cccggtggaa tttggagcgg aaccggattt
    16801 acgaatactt cttccggaac atgggatcct gcattagctg gtgctggaag tcacaacatt
    16861 acttatgcta tttctggtgc atgcggtgat actgcttctg caacaatcgt tgtcaatgaa
    16921 acgccggatg tgattgtaac ctgttttggt gaaagctgtt ttggcgccaa tgatggtttt
    16981 gcctttgtcg atatttccgg aggaaccgcg ccatatttta ttaactggga taatgctgaa
    17041 accactgata ctattgacat gcttccaccc gggacttatg atgtttcggt aagcgatgcc
    17101 aatggctgta cgcgcactgc cagcggaacg gttgatgttt cttctgattc ctgctacacc
    17161 ccgcatgttt ttattcccaa tatattttcg cccaatggag atgcaaacaa tgatgtgtat
    17221 cttgttcagg gtaagggtat tacgaatttt acattaagga tttatgatcg ttggggagaa
    17281 aaagtttttg aaacgacgga tctcacaagc ggctgggacg gagagtttaa tggcaaaccg
    17341 gtggaacagg gggcatatcc ctatgctgtg tcgcttattt ttgagggaga aacgacagtc
    17401 agggaataca acggttatat tacaattgta cgataaggag gacgaaatca tgaaaaagca
    17461 aatcacattt actttcgcca ttattctgat ttctgttttc ggaatcaggg cacaggacat
    17521 gcattattcg cagtataatg aagcaccgtt gttattaaat ccggcattga ccggctttta
    17581 cgatggcaat caccgttttg tgctgaatta taaaaatcaa tggaaaaatc tcggggctgc
    17641 atacaacaat tatgattttt cgtatgatat ttcgtttaat aaaggaaata caacaactgg
    17701 ttttcttgct ttgggaataa cggctttcaa cgatgttaca ggcgatgtgc agtttacaac
    17761 aagcggagcc atacttaata ttgcatatca gttatatctg aacgaaagca acatgcttgg
    17821 agccggcatt tatggcggtt acgtgcaaaa atcgctgagt gatgaaaata tgatgtgggt
    17881 gaatcaatat gatggtgcca ccggttttga cggcacgatc caaagcaatg aaacatttca
    17941 gaatatggat tttggtttta ctgatttcgg agcaggactt aattatagct tttcgaacaa
    18001 taccagaggt cttaagtcga atgaaggttt taaatttaat gttggcggag ctgttcagca
    18061 tgttacacag ccggcttatc agtttacagg aactgccgac gaagtggttg acatgagaat
    18121 caatgctcat ttccgttctg ttattcagat ggggagttcg aacctggctc tggtccccag
    18181 tttttattat gccatgcagg gaactcagaa ggaaatgctg ccaggtcttg gctttagata
    18241 tcaaatgcgt gaagaatcca aatataccgg atttatcaag gatgcattta tgacacttgg
    18301 aggacatttt cgcacgggtg atgcagccat tggttatctt ttggttgaat tgaatgactt
    18361 tgcgctggga gtctcatacg atgtgaatgt ttctaaactc aacaagttca ccaatagtaa
    18421 tggtggcttt gaatttatga ttcgttatat tctgaagaac gacaacagca gcagaacact
    18481 tttataatcc tgataattac taaaaaagtc aataatatgc ttttaaaaat ttcaaatttg
    18541 gggaaagttg taacagcaca actgattttt cttgtgtcag cagtgtatgt gcaggcacag
    18601 gttggtattg ccacaacttc cataacgccg cacgcttctg ccatgcttga agtgcgcgca
    18661 gcaaacatgg gaattttgat tcccaatgtt gctcttacag gaacgaccga cgcaattact
    18721 gtcgcttcgc ctgctaccag tctttttgtg tacaacactg caacggtcag tgatgtgact
    18781 cctggttttt attactggga tggcagcaag tgggtccgtt ttttaactgg cgccagcagc
    18841 gatgactgga aactggccgg aaatgccggc acaaccgcag gcactcattt tttgggtact
    18901 accgatgctg ttgatatggt tttcaaaacc aatgctgttg agcagatgcg cattttgagc
    18961 ggcggtaatg tcggaatcgg aactgcagcg ccgaccaata agctgcatgt tgatggactt
    19021 ggcactacaa cagccattta cgggcaatat gatgccaaca ggaatgccta tatggcatcc
    19081 tccagccgtg gagtatccgc ttcgatgaat accggtgctg catcgggata tggaggttat
    19141 ttctctcaca ccaacagctc ctcgtcgggg tatggcattt atgcagaaac caatttcaca
    19201 ggaactacca acccaagctg gacctatggt gcccgaatca tttcgaattc tacaacacag
    19261 gatcagtatg ggatttataa cactgcaaac catacaggta caacggggta tgtaaggggt
    19321 ttttataatg atgtggatat agctgcagca aataccagtc ttatatatgg tatcaggcgt
    19381 gtccggttta ggtaattcac aaatcacgac aaacagcttg cagagc
//
LOCUS       DAOPKE010000055        19115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3032_length_19115_cov_11.859129, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000055 DAOPKE010000000
VERSION     DAOPKE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3032_length_19115_cov_11.859129"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1315)
                     /locus_tag="PKY63_08205"
     CDS             complement(32..1315)
                     /locus_tag="PKY63_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00632.1"
                     /translation="MLNTLKIWDEKGIDSYHAAPVQTWPEAKKLNHYYTRLKDKNGSN
                     YYISHPPLAFIANFALLKFFGFPLNQSSLQWILIFIFLIEAVLLGWIIKQISQQKNLR
                     YLHLTILAALAFYLLNPVNLYAHSQHNFSEIWGQFFLITTLAAWTLYLQSDRIFIVRL
                     LLFLSAALLAATDWMGLTMMAALFIIYFKQFKKPHIRFGLIMVATAVIITSAAVFMQY
                     VSIGGLHALYKALGIRYLERSGFFGSQYTDMGYDVLNSETWLMLIKQVHNILSGPGYI
                     VVAMAIICFVFARKKCGPVDLTIQKVALLTALLFFIAVLSASSIHYIYTARFTPFIAL
                     AGAALFAKILEVFKKPGFVIGIFIFLMHLAAFWSTGIFHKSIPEPDTKQAQLNAAAVI
                     IKQNKIDAIPLTSDFVESDIIYLSYKSERNLVWIK"
     gene            complement(1443..1757)
                     /locus_tag="PKY63_08210"
     CDS             complement(1443..1757)
                     /locus_tag="PKY63_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPF00633.1"
                     /translation="MDTNNPIIQEIITRIKTVYDPEIPVNIWDMGMIYDISVDAELNA
                     KILMTVTAPNCPVAESLPIDVKTAVEYVSKLKNVWVELTFDPPWSPDLLSDEAKLELG
                     LL"
     gene            complement(1760..2194)
                     /locus_tag="PKY63_08215"
     CDS             complement(1760..2194)
                     /locus_tag="PKY63_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPF00634.1"
                     /translation="MKTITEKTAEMTAGFSNFEDWLDKYNYIIETGKSLPTLDESKKN
                     PSNLISGCQSQVWISASYENGAVFFEGDSDALITKGIAAILIGVLSGSTPDEIIQCDM
                     KFIDDIGLREHLSPTRANGLNGMIKQMKLYALAFKTKFEKQV"
     gene            complement(2191..3408)
                     /locus_tag="PKY63_08220"
     CDS             complement(2191..3408)
                     /locus_tag="PKY63_08220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPF00635.1"
                     /translation="MKQTLSEIRSQFPILSRQVNGKQLVYFDNAATTQHPVSVIEAES
                     RYYKELNANVHRGVHYLSTQATIAFEEARKSVQTFINAASENEIIFTRGTTESINLVA
                     SSFTTLMKPGDNVIITTMEHHSNIVPWQLACHRQGLELRVAKLTPDEILDVEHLKSLT
                     DEKTKLISVAHISNVLGVINPIKEIIKFAHAENIPVLIDAAQSVAHQCIDVQSLDCDF
                     LVFSGHKMYAPMGTGVLFGKEKWLDKMPPWQSGGEMIKNVSFEKTTFNELPFKFEAGT
                     PNVAGAIGLQAAIEFLQNTGLKNAADHEHELTQYAMQKFRDTANIRIFAPDTERSAVI
                     SFLINGVHPYDSGTLLDKLGIAIRTGTHCAEPLMKHLGITGTMRISFALYNSKEEIDY
                     FFESLNKVKSIFE"
     gene            3527..3997
                     /locus_tag="PKY63_08225"
     CDS             3527..3997
                     /locus_tag="PKY63_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00636.1"
                     /translation="MKKFLHILFAFLLLSSLSMGAFAESTGKKPGKLLKKFAARVESH
                     DVKGVLALLDGSYKQVQLIGMHKNDTTKFINELFCGYSTADNKTFICANISNIKKCSF
                     MRLGKFDSDTKSPAYASVNYRIDTFDGQKIKISLVVVRITTSTGVLFRLAGAVG"
     gene            4023..4514
                     /locus_tag="PKY63_08230"
     CDS             4023..4514
                     /locus_tag="PKY63_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00637.1"
                     /translation="MSDIKELKRVYLDKKTRISAVGQKIPVVAFLLMWAVLLGLMVYM
                     CITSYYLVFLIMGLIFALCGYLTFRSLRIMRKVYADDQFMYIRCKDVEEKVPFEQIYA
                     GSKPAIRLVSALEAVKFYYKTENGTKKVVKFVPAQVNQMYNQFIDAIAAKNINVKIKR
                     SFL"
     gene            4628..5158
                     /locus_tag="PKY63_08235"
     CDS             4628..5158
                     /locus_tag="PKY63_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00638.1"
                     /translation="MKDNTTISRLFLVIAIMAVFAFLRIFIEMPNISPVAAIALFGGT
                     LISRKGWAILFPLVILFASDAVLGFYSPFLMAFVYGSFALIAVVGLWLRKHMKVHNVV
                     LASLASSVLFFVVSNLGVWAEGLWYPMTVTGLTECFAMAIPFFRYELVGTLGFTLVFF
                     GVYQLATTRISAFKAA"
     gene            complement(5399..6109)
                     /locus_tag="PKY63_08240"
     CDS             complement(5399..6109)
                     /locus_tag="PKY63_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00639.1"
                     /translation="MKKICFVFLLMSVFGFAQNNHLNLHFNGGTSGFQLIDNGGTLID
                     IYDALIHEQENGMFYNATLGYSHSFGRFSFGADVSWNRLQEKSILHMDSLNAGSGNEY
                     VGLTQSDDYRYRYMNATAKVQFSMISHDKFNFGPYLQFGAAHIFSIRRKADFHFEAAL
                     DSYSIVEKKDGSDFRPLILNAAAGLFAQYKLAESWSMRADLNYMSFLQHWVKLDFPEK
                     SWYRYYSASGGIEVVYSF"
     gene            complement(6162..7562)
                     /locus_tag="PKY63_08245"
     CDS             complement(6162..7562)
                     /locus_tag="PKY63_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPF00640.1"
                     /translation="MKRGILFLLGMISLSVSAQQISIIKAETTANEFFSQNKLGCTIQ
                     GVFPRLDSSGDTLYYIVNAAPHHYVIVSASKYVSPVIAWSATSLFDESMPFAQILDKD
                     ISNRIGKASLLTNQHKNIIANQWNQLCDGISQSPKLQYWPDSGTTSTGGWVESHWTQT
                     APYNLMCPMDPATGTRSYAGCPAIAMGQIVNYLKTTQNTRFDDGDDYYHNYGTRKYDI
                     DDDADSLSFPTFPDLNEWLDSADILYQSGLDATASTAAAIVFACGTACTQVYSSAGSG
                     TYAISQAYAAYQRFGFTSCELHENTDSLMYATLISNMKSGWPAHLAVVDDAWSVGHNV
                     VVDGYRADGYFHINFGWGGSNDGWWLVPDPTFPYSMGVLEGIVLNIIPVSAGMKETVN
                     SENIDFYPNPANDMIYLNDASGANVQYFVFSSTGQLMQSDYTSGSISVSDLPAGIYFL
                     QLQTEDGMQVSKLVIE"
     assembly_gap    7745..7844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8090..9001)
                     /locus_tag="PKY63_08250"
     CDS             complement(8090..9001)
                     /locus_tag="PKY63_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00641.1"
                     /translation="MSTKSKTKRNFAVVFLFIVVVMTSCSTDNTGKLLQNTGSILASV
                     QRPPDTTLNIPWLDGDRKVYVIPANTFFSRGNILLLHGWNLPPLGWCEQTTLCKKLRD
                     AGFNIIIPDMGKSMYASQTYDETLPTMAEQPLRSWLTDTVIFHLRDSFKLFTQEANNF
                     IVGLSTGARGAFAVGIDIPEVFDGAVVLSGDYDQTLMSDDNLMTYFYGSYEEYPERWK
                     GEDNMLLNADKWTLPIYIGHAQQDRVVPWLQSDTLNKTLLKIGKVEPVTHFPKNFGHD
                     YTYWESESDNIIAFIRKICDREITSEK"
     gene            9091..12651
                     /gene="dnaE"
                     /locus_tag="PKY63_08255"
     CDS             9091..12651
                     /gene="dnaE"
                     /locus_tag="PKY63_08255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPF00642.1"
                     /translation="MSQFVHLHLHTIYSVLDGAANIKTLLKRAAALNMKAVAVTDHGQ
                     MFGILEFVSEAKKAGIKPIIGCETYVARNSMDQMSSKEDRSGHHLVLLAKNMQGYRNL
                     VKLVSQANLKGFFYTPRIDKDLLTKYHEGLIASSACLGGEIPQLIMNRGEDEALKSVE
                     WFKNLFGDDFYLEMMDHGLPEQKQVNEALVRLSQKTGVKLIATNDVHYVHKEDFEAHK
                     ILIRINTNKDDNDDLYYTGNEYLKSYDEMYSLFAEHPEALSNTVEIADKIEEFSIEHD
                     VMLPVYPTPKEFSSDYEYMAHLAREGVKNRYSEFTDEIKDRLDFELATIEKMGFAGYF
                     LIVQDYIRKARQMGVLVGPGRGSAAGSIVAYGLGITNIDPIKYNLLFERFLNPDRISM
                     PDIDVDFDDYGRDSVIDYVIKQYGAERVSQIITFGKLGAKSSIKDVARVMGVDLALSN
                     RITKLIPDGPKVTLDDAMRNKDFEDLYNSNPDVKKVVDIARTLDGNVRSTGVHACGVI
                     IGKDDLSDHVPMARAKDSILPLVQYDGSVVEKAGLLKMDFLGLKTLSIIKDALEIIKE
                     RHNKTIDIDSISLEDERTYKLYQKGETIGTFQFESEGMRSWLKKLKPTYIEDLIAMNA
                     LFRPGPMGFIPNFIARKHGVETVDYPHPLAEQVLKDTYGIMIYQEQIMLLSQRLANFT
                     KGKADELRKAMGKKKMDIIDRLRGEFIEGAKANGIEKALANEIYDTMAKFGEYGFNKS
                     HSAAYTVLAYQTAWLKANYPPEYMAAVLGHNLNDLKKVTFYISECRRMGIAVLGPDVN
                     ESSLKFSVNRENQIRFGLAALKNVGESAAQSIIDERISNGMYSDINDFFKRNNFRNVN
                     KRALESLAMAGSFDNMNSHRAQFFNMDADGVVFLEKMIKHATQMQEKMNSQQASLFGE
                     MEEATFPVMELPACDRWNLDDKLARELDVAGFYISGHPLDEFRNEVLHFSTYQIQEIS
                     DGLDVMKVGTDLLFSGVVKDLQERRDKNENLYSSFTLFDYSGEMSLRAFKDDYVKFKP
                     ILENGQKVFVYAKVDAGRFGRDPDSRELRLQNISKLAGYFDQKIAAVLFHVPMSDISA
                     DGTRRIEEIISAHPGNIIIKFLMLDPQNKEVVKLSNPYMKTDVEGISLLADLEFIIAY
                     DVKSTILNSVIPFRKKLIEEEPTAAVVEISEDVNADEYQE"
     gene            12728..13045
                     /gene="trxA"
                     /locus_tag="PKY63_08260"
     CDS             12728..13045
                     /gene="trxA"
                     /locus_tag="PKY63_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF00643.1"
                     /translation="MAHQFTDAEFEKDVLQSDVPVIVDFWAEWCGPCRMIGPIIEELG
                     TEYAEKAKVGKVNVDENPQITSQFGIRNIPTVLFFKGGQLVDKHVGAASKRIFEEKLQ
                     KLL"
     gene            13042..13968
                     /locus_tag="PKY63_08265"
     CDS             13042..13968
                     /locus_tag="PKY63_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF00644.1"
                     /translation="MIDWNDKRLQQFGYLGLRARQVVEGFITGLHKSPFHGFSVEFSE
                     HRQYNSGESTRFIDWKLFARSEKLFIKRFEEETNLRCRIIIDGSSSMLFPGDSSQPQE
                     LQSKYHFSVFLAAVFMIIFRHQRDAVGLSLVSKELETHTAAKSTFIHHQYLMSILQQH
                     LDKTIKEPSSETRLVPALHEIAERIHRRSMVIIFSDLFDAAHNPDALLDALQHLRHNG
                     NEVIIFHTLDKKFEEDFEFGNVPHKFIDLENRSVIKIRPAEVREMYQKNFAKLMNEIR
                     TKSPQYGVDYIEADLNLGLEGVLLPFFARRRM"
     gene            complement(13936..15267)
                     /locus_tag="PKY63_08270"
     CDS             complement(13936..15267)
                     /locus_tag="PKY63_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00645.1"
                     /translation="MKKNIIILFLLIFSGSAASAQNAFISADSLITLSEKWSNTDNKK
                     SLIFAKTALKISSLEKDTSTMAASLFNVGLAQYYLSDFDSALHSLQTSYYLYKKINHL
                     KGMSAAANNIGMVYHALSDFANAKKYYKISLRIDISIKDTAGMAFGLNNLGELYHFKQ
                     DYEKALHYYLLSEGIDQKAGNMYGVAASWLNIGGIYTETGNMAKGNEYYIKAIEYFTS
                     VNDKRNLPLVLTSLGDNYRLIGNFGQARKLILHAVNISREYQNDLGLIQAQIYYSKLL
                     YDENKPDSAEYWLFEALDKCVETNNNLMACEALMQQGIVLSDNKKYEEANLYLLNAYE
                     FAIDMQYNAALNDILQLLSDNYSALNDFENALKYQKLATAIDEPTNIQKTNTVEKTQT
                     NHADTSAKSIIIGLASGVVLILFLTIIFLYSKMKHYKKLAESHSSTGEKRQ"
     gene            complement(15591..17210)
                     /locus_tag="PKY63_08275"
     CDS             complement(15591..17210)
                     /locus_tag="PKY63_08275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF00646.1"
                     /translation="MRYFHALKLFFLWLLAAWLIASCSHKSDADFSKIILQQWYAGEI
                     NGEKWFFKFQSATNKTAEGIAFHDKNQSMLSPGKVSIGNDFLKINFENSSETIDGKWN
                     ISGFDLIFIKKKEGRQKTVFKLQPTFTLPATKKRYLNNIAKKVSRKEVKYGSVRGFYT
                     SKKVKDMNAESYPQIISDVLSELGNNLLTTELSLMLDIYEPQNDSATSRPLLLLIHGG
                     AFIVGDKRDSFQQKLAIHYAKRGYVVASINYRLGYLFIPGAYSNLERCIYRAVQDSRA
                     ALRYLVKHKNKYRIDPDHIFIAGNSAGGFIALKTAFMAESESYKSIEGNLLMLQEDLG
                     CIDCSGNYFKEKYRLKGVINMWGALTDIEMMDEFEQMPLLLIHGDSDAIVPYGHSYPF
                     SNVGEQYSAFFSNKVYGSEPIFKKASAMGFPVSLLTIPGGGHEPQFDEHNNYTQDLSL
                     ITAAIDSFLYAQLTINTFRIEKQTQNIFAITPVTNVDKVYWNITGGCITDIAAKNKVR
                     MVRFSDAEKCVLKAVVLNKNGLSQYIEINID"
     gene            complement(18371..19069)
                     /locus_tag="PKY63_08280"
     CDS             complement(18371..19069)
                     /locus_tag="PKY63_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00647.1"
                     /translation="MPLRGNSKELEQEERIKSGKPGSPCHKKNLALNVDKSGNNICAG
                     SNVYQAAKIKELDALNLPPEKYAVEYRKIVEKACICVGLGTSALLVNGLDTKLEGDGV
                     SVCPGPNLAYFSKEMTLQEMTDHIYNRSNVVSRKDRPHVFINELHMYLDVLKQKFESF
                     CNAENKLQPKSIQSFSQNMIDGIEYYSGLFKNMKGRFNELKTQIDIELESARQSIQTI
                     LKELEKLKMVLVVI"
BASE COUNT         5424 a         3693 c         4170 g         5728 t
ORIGIN      
        1 actttcaact cgcgcagcga tgtgccgcat gctattttat ccataccaga ttgcgctctg
       61 atttataact caggtaaatg atatcacttt caacaaaatc gctggtaagc ggaattgcgt
      121 caattttgtt ttgctttatg atgactgctg ctgcgttgag ttgcgcttgt tttgtgtctg
      181 gttcagggat tgatttgtga aaaatgccag ttgaccagaa tgcagcgaga tgcatcagaa
      241 aaatgaaaat gccaattacg aatcctggtt ttttgaaaac ctccagtatt tttgcaaaca
      301 gcgctgcgcc agctaaagca atgaatggtg taaaacgagc agtataaatg taatgaatgg
      361 aactggcact cagcaccgct ataaaaaaca gcaaagcagt caacagtgcg actttttgga
      421 tggttaaatc taccggacca catttttttc ttgcaaaaac aaaacatatt attgccatcg
      481 caacaacaat atatccggga cctgaaagta tgttatgaac ttgttttatc aacatgagcc
      541 atgtttcgga attcagcaca tcgtagccca tatcagtata ttgagaacca aaaaaaccgc
      601 ttcgttccag gtaacggatt ccaagcgcct tgtacagagc atggagtcct ccaatggaga
      661 catattgcat aaaaacagct gcagatgtga tgatgacagc agtagctacc ataataagcc
      721 cgaagcggat atgaggtttt ttaaattgtt tgaaatatat gatgaaaaga gctgccatca
      781 ttgtcaatcc catccagtcg gtagcagcca aaagagcagc agagagaaaa agcagcaagc
      841 gcactataaa aatccggtcc gattgcagat aaagtgtcca tgccgccaat gttgtaatca
      901 aaaaaaactg tccccatatt tcgctgaaat tgtgctggct gtgtgcgtaa agatttaccg
      961 gattgagcag gtaaaatgcc aatgctgcaa gaatggtgag atgcagatac cgaagatttt
     1021 tttgttgtga gatttgtttt ataatccaac ccagtaaaac ggcttcaatc aaaaaaataa
     1081 atatcagaat ccattgcaat gatgattgat tgaggggaaa gccaaagaat ttcaacaatg
     1141 caaaattagc aatgaaagcc aagggcggat gcgagatata ataattgctg ccgtttttgt
     1201 ctttgaggcg ggtgtagtag tggttcagtt tttttgcctc cggccaggtt tgtacaggtg
     1261 cggcatgata cgaatcaatc cctttctcat cccatatttt cagtgtattg agcatgaaaa
     1321 tatgagcagt gtttgcatga aaaaccaccg gactgatttt ttgaaacaga agccaaaagg
     1381 caagtattaa tagcccaata gccggtatta acaggcgaag tttgttcaag gttttttagg
     1441 aattacaata atccaagttc aagttttgct tcatcactga gcaaatcagg gctccagggc
     1501 ggatcgaatg tcagttcaac ccaaacattt ttcagtttcg agacatattc aacggcggtt
     1561 ttcacgtcga taggtaagga ctctgctacc ggacagttgg gtgcagttac ggtcatcagg
     1621 atttttgcat ttaattcagc atcgaccgaa atgtcgtaga tcatgcccat atcccatatg
     1681 ttgacaggga tttcagggtc atacacggtt ttgatccgtg taattatttc ctgaataatc
     1741 gggttgtttg tatccattgt tatacttgtt tttcaaattt cgttttgaat gccaatgcgt
     1801 agagtttcat ttgtttgatc attccattga ggccattggc gcgtgtgggt gagaggtgct
     1861 cgcgtagtcc gatatcgtcg atgaatttca tgtcgcattg aataatttca tcaggagttg
     1921 agccggataa aacgccaata aggatggctg caatgccttt tgtaatgagc gcatcgctgt
     1981 cgccttcaaa aaatacagca ccattttcat aggatgcact gatccaaacc tgcgattggc
     2041 agccggatat tagattggac ggattttttt tggattcatc aagggtgggc aaagatttgc
     2101 ctgtttcgat gatgtaattg tacttatcga gccagtcttc gaaatttgaa aagccggcag
     2161 tcatttccgc cgttttttcg gtaatggttt tcattcaaaa atggatttta ctttgttgag
     2221 agattcgaag aaataatcta tttcttcctt tgagttgtag agtgcaaatg aaatgcgcat
     2281 ggtgcctgtg attcctaaat gtttcatcaa tggttctgcg cagtgagtgc ctgtccggat
     2341 ggcaatcccc agcttatcga gcagtgtgcc ggagtcgtag ggatgaacgc cattgataag
     2401 aaatgatatg acagccgatc tttcggtatc tggtgcaaaa atgcggatat tggccgtgtc
     2461 gcggaatttc tgcatggcgt attgggttaa ctcatgttca tgatctgcgg catttttcaa
     2521 tccggtgttt tgcagaaatt caatagcagc ctgcaatcca atagcaccgg ccacattggg
     2581 tgttccagcc tcgaatttaa aaggaagttc gttgaaagtt gttttttcaa aactaacatt
     2641 tttaatcatc tcaccaccag actgccaggg gggcattttg tcgagccatt tttcttttcc
     2701 aaacagaaca ccggttccca tgggagcata cattttatgt cccgaaaaaa caagaaaatc
     2761 gcaatccagc gattgaacat ctatgcactg atgagctaca gactgcgctg catcaataag
     2821 taccggtatg ttttctgcgt gtgcaaattt gataatttct tttataggat tgataacgcc
     2881 caatacattg ctgatatgag caacactgat aagttttgtt ttttcgtcgg tcagcgattt
     2941 cagatgttca acatccagga tttcatccgg agtaagtttt gcgacacgca gttcgagtcc
     3001 ctgacgatga cacgccagtt gccatggaac aatattggag tggtgttcca tggtagtgat
     3061 aataacatta tcgcctggtt tcatcagtgt ggtaaaagac gaagcgacaa gattgatgct
     3121 ttcggtggtg ccacgggtga aaatgatttc attttccgaa gccgcattga taaaggtctg
     3181 tacggatttg cgggcttctt caaaagcaat ggttgcctgg gttgacagat aatgaacgcc
     3241 ccgatgtaca ttggcgttca gctccttata atatctggat tcagcttcta tgactgacac
     3301 cggatgttga gtagtggctg cattatcaaa atataccagt tgtttcccgt tgacctgccg
     3361 ggaaagaatg ggaaactgac tgcggatttc tgacagtgtt tgtttcatgg tgcaaagata
     3421 agaattttaa tggcgccctg cggttaacac attattgata tccatgttat atatcagaca
     3481 ttatcaatgt tcaaaaaaat cctttacttt gaagaaaaat tcaagaatga aaaagttttt
     3541 acacattttg tttgcattcc tgcttttaag cagtttatcg atgggagcat ttgcagaaag
     3601 caccggaaag aaacccggca aattattgaa gaaatttgca gcccgtgttg aatcacatga
     3661 tgtaaagggt gtccttgcat tattggatgg atcatacaag caggtgcagc tgattggaat
     3721 gcataaaaat gatacaacga aattcatcaa tgaacttttc tgtggctaca gtacggccga
     3781 taataaaaca tttatctgtg ccaatatcag caatattaaa aagtgctcat tcatgagatt
     3841 ggggaaattt gacagcgaca caaaaagtcc ggcctatgct tcggtaaatt accggattga
     3901 tactttcgat ggtcagaaaa tcaaaatcag tcttgtggtt gttcgtatta ctactagtac
     3961 tggtgttttg ttccgtctgg ctggtgccgt tggttgattc aaataaataa aaatgggttg
     4021 ctatgagcga tatcaaggaa ctgaaacggg tttatctcga taaaaaaaca cggatatcgg
     4081 cagtaggtca gaaaattccg gttgttgcct ttttgcttat gtgggctgta ctgcttggtt
     4141 tgatggtata tatgtgtatt acgtcgtatt atctcgtgtt tctgattatg ggacttatat
     4201 ttgccttgtg cggatacctg actttccgct ctctccgcat catgcgaaaa gtgtatgccg
     4261 atgatcaatt tatgtacatc cgatgcaaag acgttgaaga aaaagtgcct tttgaacaga
     4321 tttacgccgg aagcaagcct gccatccgtt tggtatcggc gcttgaagca gtgaagtttt
     4381 attacaaaac tgaaaacggg acgaaaaaag tggtgaagtt tgttccggct caggtcaatc
     4441 agatgtacaa tcagttcatc gatgccattg cggctaaaaa tatcaatgtg aaaatcaaac
     4501 gaagttttct ttgaacaatc ccaagccctt aatcagtgcg cttttgttga aattttgaac
     4561 agcaatcgac acatattttt ttcttcgacg taaaaaaaat tttacttttg cacaaaatca
     4621 aaggttcatg aaagacaata caacaatttc acgcttattt cttgttattg ccattatggc
     4681 ggttttcgct ttcctgcgca tatttatcga aatgcccaat atttctccgg tagcagccat
     4741 tgctcttttt ggaggaacgc tgatcagccg taaaggctgg gctattcttt tcccattggt
     4801 cattttgttt gcaagcgatg cggttcttgg cttctatagt cctttcctta tggcttttgt
     4861 gtatggttcg tttgcattga tagctgttgt aggtctctgg ctccgcaagc acatgaaggt
     4921 tcataatgtg gttcttgcat cactggcttc ttcagtactg ttttttgttg tttctaatct
     4981 tggtgtctgg gccgaaggtc tttggtaccc gatgacagtg accggattga ctgaatgctt
     5041 tgcaatggct attccgttct tccgttacga attggttgga actctcggct tcaccctggt
     5101 attctttgga gtttatcagc ttgccacaac ccgcatcagc gctttcaaag ctgcataaat
     5161 acaaagtcat ttttacgcaa aaggcagcat cggttccggt gctgcttttt ttcgtggcgt
     5221 agttggggaa atgaatgcag taaaaactgc cggtactttt gcaaatgcgc ttattgcaac
     5281 aaaaactaca actttctcaa acgatccgac cgtccatagt ccgcaatgca atggaattcg
     5341 ccgcggcgaa atggaagagt cggttgtggt gaagcgttgt ttttgttctg aggtttgctc
     5401 agaacgaata cacaacctca attccaccac tcgccgaata atagcggtac cagctctttt
     5461 caggaaaatc tagttttacc cagtgttgca gaaacgacat gtaattcaga tcagcgcgca
     5521 tgctccagct ttcggccagt ttgtattgcg caaacaatcc ggcagcagca ttcagtatca
     5581 atgggcgaaa atcgctgccg tctttttttt caacaatgga ataactgtcc aaagctgctt
     5641 cgaaatggaa atcggctttg cgacgtattg aaaatatatg cgcagcacca aactgaagat
     5701 atgggccaaa gttgaatttg tcgtgactga tcatgctgaa ttgaaccttt gccgtagcat
     5761 tcatgtagcg gtagcgataa tcgtcggatt gcgtgagacc aacatattca ttgcctgaac
     5821 cagcgtttaa tgaatccatg tgcaatatag atttttcctg cagcctgttc catgacacat
     5881 ctgcaccgaa agagaaacgc ccgaagctgt gtgaatagcc cagtgtggca ttgtaaaaca
     5941 ttccgttttc ctgttcatga atcagggcgt cgtagatgtc aatcagtgtt ccgccattat
     6001 caatcagctg aaagcccgat gtgccgccgt tgaaatgcag gttgagatga ttgttctggg
     6061 caaatccaaa aacagacatg agaagaaata cgaaacagat ttttttcatt tgtttattta
     6121 ttgaaatatt tatgtgcgtt tcgctttatc attaattacg gttattcaat taccagtttt
     6181 gaaacctgca ttccatcttc agtctgcagt tgcagaaaat aaatgccggc aggaaggtcg
     6241 ctcacagaaa tgcttccgct ggtgtaatcc gattgcatca actgtccggt tgaagaaaaa
     6301 acgaagtact gaacattggc tccggaagcg tcgttcaaat agatcatatc gttggccgga
     6361 tttggataaa aatcaatgtt ttctgagttt actgtttctt tcatacctgc gctgaccgga
     6421 ataatattaa gcacaattcc ttcgagtaca cccatggagt aggggaacgt tggatcggga
     6481 acgagccacc agccatcgtt tgatccaccc catccgaaat tgatgtgaaa ataaccgtcg
     6541 gcgcggtagc catccaccac tacattatga cctacactcc aggcatcatc caccacagcc
     6601 agatgtgccg gccagccact tttcatattg ctgatcagcg tggcatacat taatgaatcg
     6661 gtgttttcgt gcagttcgca actggtaaaa ccaaaacgct gataggcagc gtaggcctga
     6721 cttatagcgt aggtgccgga tcctgccgac gaatacacct gtgtacatgc ggttccgcag
     6781 gcaaatacaa tggctgccgc tgttgatgcg gtggcgtcga gtcctgattg atacagaata
     6841 tccgctgaat caagccattc atttaaatcc ggaaacgttg gaaaactgag cgaatccgcg
     6901 tcatcgtcga tgtcgtattt ccgggttccg tagttgtgat aataatcgtc tccatcatca
     6961 aagcgtgtat tctgggttgt tttcagatag ttgacaatct gtcccatggc tatggccgga
     7021 caaccggcat agctgcgtgt gccggttgct ggatccatcg gacacatcag attgtaaggc
     7081 gcagtctgtg tccagtgtga ttcgacccag ccgcctgtgc tggtagtgcc cgaatcgggc
     7141 cagtactgca gttttggcga ttgtgaaatt ccatcgcaga gctggttcca ttgattggca
     7201 ataatgtttt tgtgctggtt tgtcagtaac gaagctttac caattctgtt ggaaatatcc
     7261 ttgtcgagaa tttgtgcaaa cggcatggat tcatcgaata gcgatgtggc ggaccaggca
     7321 atgaccggtg atacatactt gctggccgac acaatcacat agtgatgcgg agcagcattg
     7381 acaatataat acagtgtgtc gccggacgaa tccagtcgtg gaaaaactcc ttgtatcgtg
     7441 caacccagct tgttctgcga aaagaattca ttggcggttg tttcagcttt aataattgaa
     7501 atctgctgtg ccgacactga aagcgaaatc atgcccagta aaaaaagtat cccccttttc
     7561 attgtgtttt ttgagcgcaa tgtagaaata tttcgtgtat tgagtataag tgtatcaaaa
     7621 ttttatacga acaggtggaa tgtgtatatg aaattttgtc aatgattaaa acttaaatga
     7681 ttaaatgact aaatgattaa atgactaaat gattaaatga ctaaatgatt aaatgactaa
     7741 atgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaaatg attaaatgac
     7861 taaatgatta aatgactaaa tgattaaatg actaaatgat taaatggcta aatgattaaa
     7921 tggctaaatg attaagtgaa ggattgtggt taatggttat tggttattgg tgagaccgcc
     7981 gtggcgaata gtgcgggaac aaggaccact aaagcttaaa aacttaagac ttaagatata
     8041 gtatgagaaa aatcctttca gcgttaagcc gcttcgcaag ccgcatgcct tatttttcgc
     8101 tggttatttc gcggtcgcag attttgcgaa tgaatgcaat gatattgtct gattcgcttt
     8161 cccaatatgt atagtcgtgt ccgaagtttt tgggaaaatg cgtaaccggt tcaactttac
     8221 caattttcag caaggtttta ttcagtgtgt cggattgtag ccagggcacc acgcggtctt
     8281 gctgtgcatg acctatgtag atgggtaatg tccatttgtc agcattgagc agcatattgt
     8341 cttcgccttt ccaacgctcc gggtattctt cgtagcttcc gtagaaatag gtcatcaggt
     8401 tatcatcgga catgagcgtt tgatcataat caccagaaag aacgacagcg ccatcaaata
     8461 cttccggaat atcgattcca acagcaaaag cgcctcttgc acctgttgag agaccaacaa
     8521 taaaattatt tgcttcctga gtaaataatt taaatgagtc gcgcagatga aaaataacag
     8581 tatctgtaag ccaggaacgt agaggttgtt cggccattgt tggcagtgtt tcgtcgtagg
     8641 tttgcgatgc atacatgctt ttccccatat cgggaatgat gatattaaaa ccggcatcgc
     8701 gcagtttttt gcagagtgtt gtttgttcgc accaacccaa tggcggaaga ttccatccat
     8761 gcagtagcag tatattgcct cggctgaaaa acgtgttggc ggggatcaca tatacttttc
     8821 ggtcgccgtc aagccaggga atattcaatg tagtatcagg cggacgttgg acagaagcga
     8881 ggatagagcc tgtattttgt agtaattttc ctgtattatc tgtggaacac gaagtcatga
     8941 cgacaacaat gaacaaaaaa acaacagcga aatttctttt agttttagat tttgtggaca
     9001 tgtaagaaaa gttttcaaca acactttttg aaagggtaaa aatatatatt tttatcgaaa
     9061 cgaaaatttc aaagaaaaag aaaggtattt atgtctcagt ttgtgcattt acatttgcat
     9121 accatttatt ctgttcttga tggtgcggcc aacataaaaa cgctgctcaa gcgggctgca
     9181 gcattgaaca tgaaagctgt agcagtaacc gatcacggtc agatgttcgg catacttgaa
     9241 tttgtgagcg aggcaaaaaa ggccggcatc aaacccatta tcggttgcga aacatatgtt
     9301 gcgcgcaaca gcatggatca gatgtcgtcg aaggaagacc gcagcgggca tcatttggtg
     9361 ttattggcca aaaacatgca gggctatcgc aatttggtca aattggtttc acaggccaat
     9421 ctgaaggggt ttttctatac tccccgcatt gataaggatt tgctgaccaa gtatcacgaa
     9481 gggcttattg cttcatcggc ctgtctgggg ggtgaaatac cgcagttgat catgaatcgt
     9541 ggagaggacg aagcattaaa atctgttgaa tggtttaaaa atcttttcgg tgatgacttt
     9601 tatcttgaaa tgatggatca tggactgccg gaacagaaac aagtgaatga agcattggtg
     9661 cgcctctcgc aaaaaacagg tgttaagttg attgccacca atgatgtgca ttatgtgcat
     9721 aaggaagatt ttgaagcaca taaaattctt atccggatca acaccaacaa ggatgacaat
     9781 gatgatttat attacactgg caatgagtat ctgaagtcat acgatgaaat gtattcattg
     9841 tttgctgaac atcccgaagc attgtccaat acagtcgaaa ttgcagataa aatagaagag
     9901 ttcagcattg agcatgatgt aatgctgccg gtttatccaa caccaaaaga gttcagcagt
     9961 gactatgaat acatggctca tctggcgcgt gaaggtgtga aaaacaggta ttctgaattc
    10021 accgatgaaa ttaaagaccg gcttgatttt gaattggcaa cgattgaaaa aatgggtttt
    10081 gccggttatt ttctgattgt acaggattat atccgaaaag cgcgccaaat gggcgttctg
    10141 gtggggccgg gccgtggaag tgctgcgggt tctattgttg catatggtct tggaatcaca
    10201 aatatagacc ccatcaaata caatctgctg tttgagcgat ttctcaatcc cgatcgtatc
    10261 tccatgcctg atatcgatgt tgattttgat gattatggac gcgattccgt tattgattat
    10321 gtaatcaaac aatatggtgc tgagcgggta agtcagatta tcacctttgg taaactcggc
    10381 gccaaatcat cgattaagga tgtagcacgt gtgatgggcg ttgatctcgc attgtcgaac
    10441 cgtattacaa aactgatccc agatgggccc aaggtgacac tcgacgatgc catgcgaaac
    10501 aaggattttg aagatttata caattcaaat ccggatgtga aaaaagtagt tgatattgcg
    10561 cgtacactcg acggtaatgt gcgcagcacc ggtgttcatg cctgtggtgt aataatcggg
    10621 aaagacgatt tatcggatca tgtgccgatg gcgcgtgcca aagattcgat tcttcctttg
    10681 gtgcaatatg atggcagcgt tgttgaaaag gctggcctgc tgaagatgga cttccttgga
    10741 ctgaagacac tttccatcat taaagatgcg cttgaaatta tcaaagaacg acacaataaa
    10801 accattgata ttgattccat ttcgctcgaa gatgagagaa cgtataaact ttatcagaaa
    10861 ggcgaaacca tcggtacttt tcagtttgaa tcggaaggaa tgcgctcgtg gctgaaaaag
    10921 ctcaaaccta cctatatcga ggatctgatt gccatgaatg ccttgttccg tcccggtcct
    10981 atggggttta taccgaattt tattgcacga aaacacggtg tcgaaactgt tgattatccg
    11041 catcctttgg cagagcaggt tttgaaggac acttacggca ttatgattta tcaggagcag
    11101 atcatgttgt tgtcgcagcg cttggccaat ttcaccaaag gtaaagctga tgaacttcgc
    11161 aaggccatgg gtaaaaagaa gatggatatt attgatcgtc ttcgcggtga atttatcgaa
    11221 ggtgcaaaag ccaatggtat cgaaaaggca cttgccaacg agatttatga cacaatggcg
    11281 aagtttggtg agtatggatt caacaaatcg cattcggctg catacacggt tctggcctat
    11341 cagacggcat ggctcaaggc caattatccg ccggaataca tggctgcggt attgggtcac
    11401 aacctcaacg atctgaaaaa agttactttc tatattagcg agtgccgtcg catgggcatt
    11461 gctgtgcttg gccccgacgt gaatgaaagc tcgctgaaat tttcagtcaa cagggaaaac
    11521 cagattcgtt tcggattggc tgcattgaaa aatgtgggag aatcggcagc tcagtctatc
    11581 attgatgaaa gaatttcgaa tgggatgtat tctgatatca atgatttttt caagcgaaat
    11641 aattttcgca atgtcaataa gcgtgcgctt gaatcacttg ccatggccgg aagtttcgac
    11701 aatatgaatt cgcatcgcgc acagtttttc aatatggatg cagatggtgt tgtttttctt
    11761 gaaaaaatga tcaagcatgc aacgcagatg caggaaaaaa tgaactctca acaggcgtct
    11821 ttgtttggtg aaatggaaga agctactttt cctgttatgg aattacctgc ctgtgaccga
    11881 tggaatcttg atgataagct ggctcgcgaa cttgatgtgg cgggcttcta tatcagtggt
    11941 catcctttgg atgaattcag aaacgaagtg ttgcatttca gcacgtatca gattcaggaa
    12001 atctctgatg gactcgatgt gatgaaagtg ggtaccgatc ttttattttc cggggtggtg
    12061 aaagatttgc aggagcgtcg cgataaaaat gaaaatttgt attcgtcgtt tactttattt
    12121 gattattcag gcgaaatgag tctgcgtgca tttaaggatg actatgtaaa attcaagccg
    12181 atacttgaga acggacagaa agtgtttgtt tatgcaaaag tagatgcagg gcgctttggg
    12241 cgtgatccgg attcgcgcga attgcggctg cagaatattt caaaactggc aggttatttc
    12301 gatcagaaaa tcgcagctgt gctttttcat gttcctatgt ccgatatttc ggccgatggc
    12361 acacggcgca ttgaagaaat aatcagtgcg catcctggaa atataattat caaatttctg
    12421 atgcttgatc cgcaaaacaa ggaagtggtc aaactgagca atccttacat gaaaaccgat
    12481 gtagagggca tttcattgct ggccgatctg gaatttatta ttgcttatga tgtaaaaagc
    12541 accattctta actcggtgat acccttcagg aaaaaactga ttgaagaaga accaaccgct
    12601 gctgtggttg aaatttccga agatgtcaat gctgatgaat atcaggaata gcaattaaaa
    12661 acggatttgt tttttcgtca gatttgagta attttgtatt tgttctaacc ttaaacctaa
    12721 aatatttatg gcacatcaat ttaccgatgc tgaattcgaa aaagacgttt tgcaaagcga
    12781 cgttcccgtt attgttgatt tttgggcaga atggtgcgga ccttgccgca tgattggtcc
    12841 gatcatcgaa gaacttggga cagagtatgc agaaaaagct aaagtcggca aggtcaatgt
    12901 cgatgaaaac ccacagatta cttcgcaatt tggcatcaga aatattccga ctgttttgtt
    12961 tttcaaaggc ggacaactgg ttgataaaca tgtcggagct gcttcgaaac gtatttttga
    13021 agaaaaattg cagaaactgc tttgatagac tggaatgata aacgtctgca gcagttcggc
    13081 tatctcggat tgcgtgcacg acaggttgta gaaggattta taacggggct tcacaagagc
    13141 ccctttcatg gtttttcggt tgaattttcg gaacacaggc aatacaattc aggtgaaagc
    13201 acgcgtttta ttgattggaa attatttgcc cgaagcgaaa aactttttat caagcgtttt
    13261 gaagaagaga ccaatctgcg ctgtcgcatt ataattgatg gatcgtcttc catgttgttt
    13321 cccggcgaca gcagccagcc gcaggaattg caaagcaaat atcatttttc ggtttttctg
    13381 gctgcagtgt tcatgattat tttccggcat caacgcgatg ctgtcggact ttcgctcgtt
    13441 agcaaagagc ttgaaacgca tacggctgcc aaatcaacat ttattcatca tcagtatctg
    13501 atgagtattt tgcaacaaca tcttgataaa acaatcaagg agccgtcttc tgaaactcgc
    13561 ctggtacctg ccttacatga aattgctgaa cggattcatc gtcgttcgat ggtgattatt
    13621 ttcagcgacc ttttcgatgc agcacacaat cccgatgctt tgcttgatgc tttgcagcat
    13681 ttacgacaca acggaaatga agtgattatt tttcatacgc tcgataaaaa attcgaggaa
    13741 gattttgaat tcggcaatgt gccccataag tttattgatc tcgaaaaccg ctctgtgatt
    13801 aagatacgtc cagctgaggt cagagaaatg tatcagaaga attttgcaaa actgatgaac
    13861 gaaattcgca caaagtcacc gcagtatggt gttgattata tcgaagcaga tctgaatctt
    13921 ggactcgaag gcgttttact gccttttttc gcccgtcgaa gaatgtgatt ccgccagctt
    13981 tttgtagtgt ttcatttttg aatacagaaa aataattgtc agaaacagta tcagtacaac
    14041 tcctgatgca agtccaataa taatactctt cgctgatgtg tcagcatgat ttgtttgtgt
    14101 tttttcaacg gtgtttgtct tttgaatatt tgttggctca tcaatggcag tcgccagttt
    14161 ctggtatttc aaagcatttt caaaatcatt cagtgcagaa taattgtcgc tcagtaattg
    14221 caaaatgtca ttcagtgcgg cattgtactg catgtcgatt gcaaattcat aggcattgag
    14281 caaatacagg tttgcttctt cgtatttttt gttgtccgac agcactatgc cttgttgcat
    14341 cagagcttcg catgccatca gattattgtt tgtttcaacg catttgtcaa gggcttcaaa
    14401 aagccagtat tcggcgctgt ctggtttgtt ttcatcatac agaagctttg aatagtaaat
    14461 ctgtgcctga attaatccca agtcattctg atattctctg ctgatattca cggcatgtaa
    14521 aatcagcttt cgcgcttgac cgaaattgcc gatcagccgg taattatcgc ccagacttgt
    14581 caataccagt ggaaggttgc gtttgtcgtt tactgaagtg aaatattcaa tggcctttat
    14641 gtagtattca tttcctttcg ccatattgcc ggtttcggta tatatgccgc caatgttaag
    14701 ccaggaagca gcaacaccgt acatattgcc tgctttttga tcgattcctt ctgaaagcag
    14761 ataataatgc agtgcctttt cataatcctg tttgaaatga tagagttcgc ccaggttgtt
    14821 caaaccaaaa gccatgcctg ctgtatcttt gattgaaata tcaatgcgga gactgatttt
    14881 gtaatatttt ttggcattgg caaaatcact taatgcgtga tacaccatgc ctatattgtt
    14941 ggcggctgcc gacatccctt ttaaatgatt tattttttta tacagataat agcttgtctg
    15001 taaagaatgc agcgcgctat cgaaatcact cagataatat tgcgccaagc caacattgaa
    15061 gagacttgca gccattgttg acgtgtcttt ttccagagat gaaattttca atgcggtttt
    15121 cgcaaaaatc agacttttct tattgtcggt attactccat ttttccgata aggtaatgag
    15181 tgaatcagcc gaaatgaatg cattctgtgc tgaggcagca gaaccagaaa aaatcagcag
    15241 aaataatatt ataatatttt tcttcatgaa taaacaaaaa tacaatcttt tttgaatttt
    15301 gatgatatta aacagaagcg acgtataaaa ctgccggccg atgccagagg gatcgcatat
    15361 tatcagtatt gtcagaatat caagggcctg cgaaaaaaca cgtaataccg acgaaaacta
    15421 ctcctgtcgg tattaataaa atgaaataca tgattttttt tagattatca tatattcaaa
    15481 ttgacataat tattcatttt tgtcttgtcg cctcgaatat agcgcagctc cgactttttc
    15541 agcaggcctt atatagtttt ttatttgcga ttcagcattt caaaaaaaaa tcagtctatg
    15601 ttgatttcaa tgtattgcga caaaccgttt ttattcagca caacagcttt gagtacgcat
    15661 ttttccgcat cgctgaaacg caccattcgt actttatttt tggctgcaat gtcggtaata
    15721 cagccgccag tgatgttcca atagacttta tcgacgtttg tcactggtgt tattgcaaag
    15781 atattctgtg tctgtttttc aattctgaaa gtgttaatgg tcagttgtgc gtacagaaaa
    15841 ctgtcgatgg ccgctgttat gagtgacaag tcctgcgtat agttgttgtg ttcgtcaaac
    15901 tgcggttcat ggccgccacc gggaattgta agcagtgaga cgggaaatcc catcgctgaa
    15961 gcttttttaa atatgggttc agaaccatat actttatttg agaaaaaagc tgaatattgt
    16021 tcgccaacat tgctgaaagg atagctgtgc ccgtaaggga cgatagcatc tgaatcgccg
    16081 tgaatgagca gtagcggcat ttgctcgaat tcgtccatca tttcaatatc ggtaagtgca
    16141 ccccacatat tgatgacgcc tttcaaccga tatttttctt taaaataatt tccagagcag
    16201 tcaatacatc ccaggtcttc ctgaagcatg agcaaattcc cctcaatact tttataggat
    16261 tcggattcgg ccatgaatgc cgttttcaag gcaataaaac ctccggcact gttgccggct
    16321 ataaaaatat gatcagggtc gatgcgatat ttgtttttat gtttcaccag atatcgcagt
    16381 gctgcacgtg agtcttgtac cgcacgatag atgcatcgtt cgagatttga ataagctccc
    16441 ggaataaaca aataacccag ccggtaattg atgcttgcta ccacgtaccc acgttttgca
    16501 tagtgaatgg cgagtttttg ctgaaatgaa tcacgcttgt ctccaacaat aaaagctccg
    16561 ccgtgaatca gcagcaggag cggtcttgat gtggctgagt cgttttgggg ctcatatatg
    16621 tcaagcatca gcgaaagttc tgtggttagc aaattgttgc caagctcact cagcacgtca
    16681 gaaatgattt gcggatatga ttccgcattc atgtccttga ctttttttga tgtgtaaaaa
    16741 cctcttactg agccatattt aacctctttt cgtgaaactt tttttgcgat attattcaga
    16801 tatctttttt ttgtggcggg taaagtaaaa gtgggttgta gtttaaaaac cgttttttgg
    16861 cgaccttctt ttttcttaat aaaaatgaga tcaaagcccg aaatattcca tttcccatcg
    16921 attgtttctg atgagttttc aaaattgatt ttaagaaaat cgttgccaat gcttactttt
    16981 ccagggctta gcatcgattg gtttttgtca tgaaaagcta ttccttctgc cgttttgttt
    17041 gtggcggatt gaaatttgaa aaaccatttt tcaccattta tttcgccggc ataccactgt
    17101 tgcagtatga tttttgaaaa atcagcatcg gatttatgac tgcatgatgc aatgagccac
    17161 gcagccaaaa gccaaagaaa aaacaattta agggcatgga aatatcgcat atacaaacat
    17221 aaaaattttt tctaatataa tcaatattat tggtcagggt caacgcattt aaatgcaaac
    17281 gaatacttct gattgatgca tctaatggta ccttcaagca tggaacatca accttttttt
    17341 taatgaaatt caggaagttt tatgcatgtt ttaatgcgtt gtccccgtgt tattaatgaa
    17401 tctgaaatca taataaaatt gaaactccga aatatggtca gaaaaagagt tcaaaatcat
    17461 gatagttaca ttttattgca ctctacccgt cagatgcatg cattcggttc aatcattttg
    17521 gatgactccg gatgtttcat tgaataaacc attccgtacc tgacggcacg agctggcgtt
    17581 cttttttgtt ctaccaatat tttgtcccta cgggacaata gcagcttagt ctgctgtttc
    17641 ccttctggct acgaattccg agacaaattc gcgttgcttt tgttgagctt aaataacgcc
    17701 aaagcatttt atacatcggt gccatcattt ttggaagatt ccggaggagt cgcatatctg
    17761 tagcccataa atacacaaaa ccacgactcc ggaggagtca cacataaaaa tctccgtatt
    17821 atatgcctgg catactctta taccaaaacg attgatacat tattgcaatc aattttggaa
    17881 gactccggag gagtcacata tctgtagccc ataaatacac aaaaccacga ctccggagga
    17941 gtcacacata aaaatctccg tattatatgc ctggcatact cttataccaa aacgattgat
    18001 acattattgc aatcaatttt ggaagactcc ggaggagtca catatttgta gtcaatgaat
    18061 acacaaatga cgactccagc ggagtcgcat ataaaaagct acgtcctctg acaatgcatg
    18121 tatatatgcg ccctcgcaga ggttgatggt gtttagccgt tttgaaaata taatactgat
    18181 tacgcttttc cgacataaag aaagttgatc agaaaaaata ttggtagcaa ccgaaaacaa
    18241 catgattccg tgcaggtaca ggacaatttt attcaatggg attgtttcga tgccaagttt
    18301 tcgcggcagg cacggagaga tgtaattctt cttgtaagtt aatcacataa aagattacag
    18361 aatcaaaaaa tcaaatgaca acaagaacca ttttcagttt ctcaagttct ttgagaatag
    18421 tctgaatgga ttgtcttgca ctttcaagtt caatatcaat ctgcgtttta agctcattga
    18481 agcggccttt catattcttg aaaagtccgg aataatactc gatgccatca atcatatttt
    18541 gcgaaaaaga ctgaatggat ttcggctgca gtttattctc agcattgcag aaagactcaa
    18601 atttttgttt gagaacatcg agatacatat gcagttcatt gataaaaaca tgaggccggt
    18661 cttttcttga aacaacattg gagcggttgt aaatatgatc agtcatttcc tgaagagtca
    18721 tttctttgga aaagtaagcc agatttggac caggacaaac agagacgcca tcgccttcaa
    18781 gttttgtatc caggccattc accagcagag ccgaagttcc aagtccaacg caaatgcatg
    18841 ctttttcaac aatcttgcga tattcaaccg cgtatttttc aggaggaaga ttaagagcgt
    18901 cgagttcttt tattttagcc gcctgataaa catttgatcc ggcgcagata ttgtttccgc
    18961 ttttatcgac attgagtgcc agatttttct tgtggcacgg actgccaggt tttccgcttt
    19021 tgatgcgttc ttcctgttcc agttccttgc tgtttcctct taagggcacc tctaaaaact
    19081 tgtttttttc ttagtgatgc gatgcaaata taagc
//
LOCUS       DAOPKE010000056        18678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3116_length_18678_cov_11.293078, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000056 DAOPKE010000000
VERSION     DAOPKE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_18678_cov_11.293078"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..626)
                     /locus_tag="PKY63_08285"
     CDS             complement(117..626)
                     /locus_tag="PKY63_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00648.1"
                     /translation="MKIVNKLFLILAVSALLSACGKYDDGPAVSFRSAEKRLIGLWEF
                     SSIKIDGIEYINDYYNDSTDLKISMSGSREEIYAILVEENRSSSQLSSSLVEMNEKST
                     EFSMGLPVSPVYNNVTQYFYALIPAFAEKNIYTIRRLSSSELWISTSFNNQVYDLQLN
                     KLEQYIFNP"
     gene            complement(610..1578)
                     /locus_tag="PKY63_08290"
     CDS             complement(610..1578)
                     /locus_tag="PKY63_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPF00649.1"
                     /translation="MSVTERVFDLQFIDHAAFYGVGDAHLNQIREIFPKLKIVARGDM
                     VKVLGPKGEIEEFSRRFENLLKHFDVFGDLKSNDIAQIMGVEAVFAEPMAQMPADVIV
                     YGRSGQPIKARTRNQLKMVEKIQSSDMLFAVGPAGTGKTYTAVALAVKWLKEKKVRRI
                     VLTRPAVEAGENLGFLPGDLREKLDPYLQPLYDALNDMLPQQKLLGYFEDGTIEIAPL
                     AFMRGRTLEKSFVILDEAQNATRMQMKMFLTRMGQDSKFIITGDMTQIDLPRTQASGL
                     IHAVNMVKNIPEIAVVVLDKSDVIRHPIVTKIINAYETEQPNENSK"
     gene            1638..2414
                     /locus_tag="PKY63_08295"
     CDS             1638..2414
                     /locus_tag="PKY63_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPF00650.1"
                     /translation="MNLNPVTITSDFGIRDFYAAVVNGRLLSLQPALNIVTVTHQIRQ
                     YDVFQASVIVRNMWNSFPDGTVHLVLVNGTSGNQDRQVAIKYKNHFFVGPDNGLHGLV
                     FDENPEIIVHLNSNPIEHETFPELDVCTKAAVHLAEGKDILELGSKSDNLLESMPFRP
                     TSDNNTIKGMVIYIDNYHNAITNITKKMFDKMNKKNKYIIRFGKYFTQKISPTYNFAP
                     EAELVAVFGSTGLLEIALYQTSARELLGIRVNDVVRIEFE"
     gene            2818..3549
                     /gene="gldF"
                     /locus_tag="PKY63_08300"
     CDS             2818..3549
                     /gene="gldF"
                     /locus_tag="PKY63_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPF00651.1"
                     /translation="MYALFKKEISSFLHSLIGYIVICVFLAANGLILWVFPNSFNILG
                     FGYATLDGLFILAPFVFLFLLPAISMRSFADEINTGTIELLMTKPISEMKIIAAKYLA
                     GIVILLFSLLPTLIYLNTISQLAVPKGNLDFGGIWGSYIGLLFLGASFLAIGIFASSI
                     SKNQIVSFVIAVFLTAFMYLGFEFIYDSGIFKGAELFIKSIGINEHYISMSRGVIDSR
                     DLLYFLSLIALFLMLTRLSLERRKW"
     gene            3555..5270
                     /gene="gldG"
                     /locus_tag="PKY63_08305"
     CDS             3555..5270
                     /gene="gldG"
                     /locus_tag="PKY63_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPF00652.1"
                     /translation="METKTNHGSNRKKSLIRLLAGIAILIVINLAGTLGFFRLDLTQE
                     KRYTLSDNTIDLLKKVDDIVYFRIYLEGELPASYTKLRNAIRETLDEFRAYNKANIQY
                     EFIDPTEGKDKRTLNEYYNELVEKGLQPSVDRQQSSSSSEQRIIWPGALVSYKTRETA
                     INFIQTGQQTDKEVLINESLESLEFNLIDAIRRLFVRKKPNIAFIDGHGESSAMMTED
                     ITRSLTDYYNVQRVTINQQLKSLDKFKTIIIAGPDSVFDEKDKFIIDQFVMDGGRVLW
                     LVDGTATNMDSLQSSSETVAVANEINLEDMLFKYGVRINNNLVLDMNSCPIPVKTGQI
                     GDQPQFEYFNWFYFPSISNTDGHPIVKNLNAIRFEFVSGIDTVGSGGIKKTVLLTSSQ
                     YSRIVNTPAVISLRMLTETPGREFFNKPKIPVAVLLEGNFSSVFTNRIPPEIAEDKEI
                     GFSESGKPSKMIVVSDGDIINNQFVYRNGQYFTYPLGYDRFTGINYGNKDFLLNCMNF
                     LTDESNLLDIRSRELKIRLLDKSLVTSNQTYIQWSNVLGPVLIIVAIGLILMIFRKIK
                     LKRSI"
     gene            5267..6367
                     /locus_tag="PKY63_08310"
     CDS             5267..6367
                     /locus_tag="PKY63_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPF00653.1"
                     /translation="MKKWLKKWYFWLPLLLIVAVFLTVFITNRSNTTLNPGEKNFSIT
                     DTASVTKVFLADKKNNSVLLERNDSNGWILNKKYSVQPEMIEELLYTLMYITVRNPVP
                     KTQYDAVYKQLAGFATKVEVYAKVYRIDLMGIKLFPYEKCIRTYYVGSATMDNTGSYM
                     LMEGAEMPFIMHIPGFRGFLEPRYSPREQDWRDHKVFRYNLTDIAKINVTYPKYADRS
                     FELLNPDNINFSVKNEINGKLENIDTVKVINYLNAYTDARFEEFLTNLPIQKQDSIRK
                     SAPFCIITVTDRKNETNSISLYRIKLPEGSTNISGEPIEFDPERLYGIVNGKDLVSAQ
                     YFVFGPLMRYANEFLDSDGAPTNRSGEFDTLF"
     gene            6431..7555
                     /gene="dnaN"
                     /locus_tag="PKY63_08315"
     CDS             6431..7555
                     /gene="dnaN"
                     /locus_tag="PKY63_08315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPF00654.1"
                     /translation="MNFIISSNILLKGLQNVSGLLSSNSLVPIIENFLFSTDGTKLLV
                     TATDNETRAVVELSPEKINGTGAVAIPPRVLLETLKTFPNIPITINVNEETFAVEISA
                     GDGVYRLAGYNPENYPSIAAIENAKSISMSSIVLQNAVSSTIFACGNDEIRPQLMGVF
                     FEITPDYTNFVATDAHKLVRFRRNDIKSDEAHSFIVHKKPLNLLRNLLNGFDCTVSVE
                     FNNTNVSFRFENYQVVCRLLDGKYPAYEGVIPKDNNYKLVINRNSLISTLKRVAIFAN
                     QASHQVRFDVKGQELNISAEDIDYSNEAKERLNCIYDGADIEIGFNSKFFLETLQTLA
                     CENITVTLSSPTRAALLIPEETNSAEEDVLMLIMPIMLNA"
     gene            7643..8305
                     /locus_tag="PKY63_08320"
     CDS             7643..8305
                     /locus_tag="PKY63_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00655.1"
                     /translation="MKRLNTFFAFILLSTIVMAQQDKLQETLAADTILYQIYHHLPTG
                     WTMSMDDTSITIYRIEKYTHVESDCSTMSLDSLAALPKNETALIHFLYENKWDAERLF
                     WTRESNDSLNMILGSLPQQMGISHLYDAEKSTRSNRVYTGKNKTEKEKINAYYKRRAQ
                     LVQQITAIPTFNTSLFSLRQRKQTALQRPGVCIYPFGVYKEMLGVYIVIMDYCKNPLD
                     EK"
     gene            complement(8661..8990)
                     /locus_tag="PKY63_08325"
     CDS             complement(8661..8990)
                     /locus_tag="PKY63_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00656.1"
                     /translation="MKNLFVIAALAGMFALVSCGGSKKDDEKAKQDSIRKADSLAKVA
                     EDSIQKAEELEQARLDSIAKAKEDSIANAAKQGTGTYKPKTNTTTVSTNTATVTTNTG
                     KPVRGGR"
     gene            complement(9023..9223)
                     /locus_tag="PKY63_08330"
     CDS             complement(9023..9223)
                     /locus_tag="PKY63_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00657.1"
                     /translation="MKNLFAIVALVGMFALVSCGGSKKDDDKAKQDSLKKDSIMKDSI
                     KKAEELAADTMNADSAKAEEVK"
     gene            9323..10012
                     /locus_tag="PKY63_08335"
     CDS             9323..10012
                     /locus_tag="PKY63_08335"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HPF00658.1"
                     /translation="MKKTFLKFAVAIAAVAFIGVSCTEDDITNPTITITGSAAVTIDL
                     GDTYTDAGATANDDKDGDLTSQITTTGTVDVNKVGIYEIEYTVSDEAGNTTTETRTVT
                     VQANRLAGSYNVSCVITGAGAGNYNFVEVVTGSSVNYNYIYFADFCGFPGLQVQGTVS
                     GAGITFNQTKNYDWDDDGTATDATITGSTLSAFTVTAGATPIAALSSLTYVIDYGASQ
                     MDNCTSTYTKQ"
     gene            10081..11988
                     /locus_tag="PKY63_08340"
     CDS             10081..11988
                     /locus_tag="PKY63_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPF00659.1"
                     /translation="MKQSPQLRLLIRYLIYFSGVLLLITLVRLLLWAFNTHLFNPASA
                     KTFFNVLFGGGLFDFSTFCLVHLIFIPFLLVPFAWLNKKVFRIGRYTLLLLAGFVLLA
                     PNIIDVIYYRFTLKRTTFDVFSFIGTMENEIGGLSLRFLIDFWYMFIVFIVLFVGYIF
                     LIKRTILNKKNREIAVKYKYQLPVALLFSAILIMGGRGSLDMRPLGILHASRVAGPEY
                     APLVVNSTYTIIKTYGKKDLEKKDWFSEMELPGIFNPVHYYYSETDSTQPLNVVIIIS
                     ESLSAEHCGFLNGGLPSYTPFLDSLSKHCLVLENMYANGKRSLDGIPAIISSLPPLMR
                     NPFVTSMYSGNEIEGVGSMLGDQGYQTAFFHGGFNGTMNFDGYAAAAGYDQYFGKSEY
                     PGPESDFDGRWGIFDEPFLQYMAEKCNNFKEPFTSCVFTLSAHHPYTIPDQHKGKFST
                     GPKPILETIGYADFALKKFFETAAKTNWYKNTLFIITADHTSELYSECYTSVPCNHAI
                     PMLWYYPGDSMLKGRSDVLCQQADILPSIADYLNLNDTIIAFGNSIFDSQSKRFVIML
                     NDVVYQMFTDGLQKSEFDGETNFRQSDYKCCSKTENTITTNTATYNKRLIKAIIQQFN
                     NRTISNRLKKP"
     gene            11985..12875
                     /locus_tag="PKY63_08345"
     CDS             11985..12875
                     /locus_tag="PKY63_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPF00660.1"
                     /translation="MMHRQSIIIINPIAGTRRIDGVENLIRNWIDLHQINCRIETTKY
                     RGHARELASDAVSEGFNHFIVAGGDGTLNEVASALYLQQNDVTLGIIPVGSGNGLALH
                     LRLPKKTEKALDIAFGEKTLSCDVGLFNNIPFFSVAGVGFDAMVAYRFSQMKGRGLRN
                     YIRAVMQNYPGFKPSDYCVEAEGKKITGRFLMISFANSNQFGYNTSLSPEASISDSLI
                     DICLMKKVPIPAAALLSPLLFLKKMNQTRYLKIIRTKKAQITASLPAYFHIDGDPYIL
                     EKADILIETLPAALNIHVKH"
     gene            12878..13243
                     /locus_tag="PKY63_08350"
     CDS             12878..13243
                     /locus_tag="PKY63_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPF00661.1"
                     /translation="MSKQKISGFEGLKNLVYSTSSETMKDIENNRQPQEPDTPDPLQQ
                     NLRVWIEKNHRGGKVVTIIKGFEGSDQALKELGKKLKNHCGTGGSEKDGEIILQGHQA
                     DKIMKYLADNGYKAKRAGG"
     gene            complement(13459..13791)
                     /locus_tag="PKY63_08355"
     CDS             complement(13459..13791)
                     /locus_tag="PKY63_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00662.1"
                     /translation="MKWIVLLLLVFSFALSSCKCNKEAKKSDTNVTVIMGSTIGTVSH
                     KFRATGCKTVIFVNQSEQVLIPIEGLPQDLDVDGRVITFDFNLLRMPQPEGCNIGQPA
                     QLSNVAAK"
     assembly_gap    13846..13945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14576..15556)
                     /locus_tag="PKY63_08360"
     CDS             complement(14576..15556)
                     /locus_tag="PKY63_08360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPF00663.1"
                     /translation="MFGKRAKYIIDPITLSLHSVEKSGKYLTKKLLSGLGLSVLVGIA
                     IAIAFFIFTESPQENRISRDINYYRLRLELLNSKSDKLISILNTLQSKDDRTYRTIFA
                     MDPLPEDERNPGVGGPSTVYSQFNVVENGHLLKETLSKLDLISRKIVVQSKSFQDLSV
                     MIQNKEKMLASVPSIMPVDRNRVKLSSGFGWRRNPFTRAGSQFHPGIDLAGKIGTPIY
                     ATGDGVVIDPLGSMTGYGIVIVIDHGYGFRTLYAHLSKKLVKEGEAVKRGQIIGLLGN
                     TGPSTGPHLHYEVIRNGQKVNPINYIYSGFTNEEFQQLIKEAEENSKILS"
     gene            complement(15664..16176)
                     /locus_tag="PKY63_08365"
     CDS             complement(15664..16176)
                     /locus_tag="PKY63_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPF00664.1"
                     /translation="MEFLAILISIPWTDILWISLGVILTMLGILGCFLPIIPGPPLSF
                     AALLLLQLKEEPPFSVTFLLIMAGVAIFVTVLDYVVPVAGAKKFGASKAGVWGSTIGL
                     ILGIFILPPWGIIVMPFIGALIGEFIIGKQSKQAFRAAWGTFLGFLTGVLLKLIASGV
                     MAFYFFSHIN"
     gene            16517..16894
                     /locus_tag="PKY63_08370"
     CDS             16517..16894
                     /locus_tag="PKY63_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPF00665.1"
                     /translation="MKKIVHSEKAPKAVGPYSQAVQTGNMLFVSGQIPMDAETGKIVE
                     GGITEQTHQVLKNMGHILREAGYDYSDVVKCTVLLDNMDNFAPMNEVYGQYFTENAPA
                     RAAYGVVRLPLGVMVEIECIAAK"
     gene            16907..17743
                     /gene="lgt"
                     /locus_tag="PKY63_08375"
     CDS             16907..17743
                     /gene="lgt"
                     /locus_tag="PKY63_08375"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPF00666.1"
                     /translation="MLNYIVWNVDPILFRLGSLEVRYYGLLFALGFLVGYYIIKYFFN
                     KENAPQIEIDKMALYIILGSVIGARLGHCFFYEPDIYLADPIQILFVWQGGLASHGGA
                     IGLIFGLWLYSLKSWNHSWLHILDRIAVPTALAGSFIRLGNLFNSEIYGYQTDLPWGF
                     VFVRDGQTMPHHPTQIYESLGYLLIFIVLLFMYNKRNGIINRGFLVGWAITLIFILRF
                     FVEFIKNDQVGFEQNMILNMGQLLSIPFILCGIALILISPRLGPAPEYMPDKGGSKKK
                     EK"
     gene            complement(17744..18574)
                     /gene="ispE"
                     /locus_tag="PKY63_08380"
     CDS             complement(17744..18574)
                     /gene="ispE"
                     /locus_tag="PKY63_08380"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPF00667.1"
                     /translation="MLCFPKAKINLGLQVLRKRPDGYHDIETVMVPVSLHDALEVTKT
                     APRTDIRMYGTDLFVPVEQNLVFKAWKLLADRHKIKPVIFHLAKSIPAGAGMGGGSSD
                     AAATFVLLNNFFRLELSNDVLKTYAAELGSDCAFFIDSKPALAQGRGEILKSIELDLS
                     NYAIAIVKPLKMSDGQTGISTPVAYSLVKPDVARIPVSEVISLPVSEWRNRLINDFEK
                     PILQKYPEIVGIISKLYNAGAVYAAMSGSGSACFGLFETKNPTLNFSRDFFVHIAKMQ
                     "
BASE COUNT         5593 a         4000 c         3644 g         5341 t
ORIGIN      
        1 ctcacaatca ttatcaataa atcaccgaca cgatatacgt cacgcacttt tccgttataa
       61 acagtctgcg acgtgctgaa ctgaaactga gtttttacga ggtctttcat aagtttttat
      121 ggattgaaaa tgtactgttc aagtttgttc aattgcaggt catagacctg attattgaaa
      181 gatgtactta tccaaagctc gcttgatgac aaacgacgga tggtgtaaat atttttttcg
      241 gcaaaggccg gaatcagcgc ataaaaatat tgtgtaacat tgttgtaaac aggactcacc
      301 ggcaagccca tcgaaaattc cgtacttttc tcattcattt ccacaagcga cgatgataat
      361 tgcgatgagc ttctgttttc ttcgacaaga atggcataaa tttcttcccg gctaccgctc
      421 atcgaaattt tcaaatcggt tgaatcatta taataatcgt ttatatattc aattccatca
      481 attttaatgg aagagaattc ccatagacct atcagccgtt tttcagcaga acggaaactg
      541 acagcggggc catcatcgta tttaccgcag gcagaaagta atgcagagac agcaagaatc
      601 aaaaataatt tatttactat tttcattggg ttgttcggtt tcgtaagcat taataatttt
      661 tgtaactatc ggatgacgaa tcacatcgct tttgtcaaga acgaccacag caatttcagg
      721 aatattcttc accatgttga cagcgtgaat caggccactg gcctgagttc gcggaaggtc
      781 gatctgtgtc atatccccag tgataatgaa tttggaatcc tgccccatac gagttagaaa
      841 cattttcatc tgcatacggg tggcattctg ggcctcatca agaatcacaa aacttttttc
      901 cagcgttcgc ccacgcataa atgcgagggg tgcaatttca atagtaccat cttcgaaata
      961 gcccaacagt ttctgctgcg gcaacatatc attcagtgca tcatagaggg gctgaagata
     1021 aggatcgagt ttttcgcgca gatcacccgg gagaaaaccc agattctctc cagcttcaac
     1081 agcaggtctg gtaaggacaa tgcgacgcac tttcttttct ttaagccact taacagccaa
     1141 tgctacagca gtatatgttt ttcccgttcc ggcaggaccc actgcaaaca acatatcgga
     1201 tgattgtatt ttttcaacca tctttaactg attgcgggta cgtgctttaa tgggctgacc
     1261 gctgcgacca taaacaatta catcggcagg catctgagcc attggttcgg caaaaaccgc
     1321 ttcaacgccc atgatttgtg caatgtcatt tgactttaaa tcgccaaaaa catcaaaatg
     1381 cttcaacaaa ttttcaaacc gtcgcgaaaa ctcttcgatt tcgcctttcg gaccaagaac
     1441 tttcaccata tcgcccctgg caactatttt caatttggga aatatctccc ggatttgatt
     1501 cagatgcgca tcgcctaccc cataaaaagc agcatgatcg atgaactgca ggtcaaaaac
     1561 acgttctgtt actgacattt atttcaagat tcgtgatgaa ttacaaaatt tgtaattttg
     1621 aaacaaaaat actgattttg aatttaaatc cggtaacaat tacaagcgat ttcggtatcc
     1681 gcgattttta tgcggcagtg gtcaatggac ggctcctttc gctgcagccg gcactcaata
     1741 ttgttaccgt gactcatcag atacgacagt atgatgtgtt tcaggcctca gtcattgtgc
     1801 gcaacatgtg gaacagcttt cccgatggaa cagtacatct ggtgctggta aacggaactt
     1861 cgggcaatca ggaccggcag gtcgccatta aatacaaaaa ccattttttt gtcggacccg
     1921 ataatggact acatggattg gttttcgatg aaaatcctga aattattgtt catcttaatt
     1981 caaatcccat tgagcatgaa acttttccgg agctggatgt ttgtaccaag gcagcagtgc
     2041 atctggccga aggaaaagac atactggagc tcggttcaaa atcagacaat ctgctcgaat
     2101 ccatgccgtt tcgcccgaca agcgacaata ataccattaa gggcatggta atttatattg
     2161 acaattacca caatgccatt acaaacatca ctaaaaagat gtttgacaaa atgaataaaa
     2221 aaaataagta tattattcgc ttcggaaaat actttacgca aaaaatttcc cctacttaca
     2281 attttgctcc cgaagctgaa ctggtggctg ttttcggcag taccggactg ttggaaatag
     2341 cgctttatca aacttcagcc agagaactcc tgggcattcg tgtgaatgat gttgtgcgca
     2401 ttgagtttga atagactaat tgaaaggttg aggttgaggc tgaggttgag gttgagtagc
     2461 tatcagcttc cagcgaagtc ccgtctgcag gatacgcaat catcagctgg taagctttga
     2521 gatgtttttt tgaaattcac aggccttgtt cgttgcgcgc tgtgccttgt agcattctct
     2581 ccaactcact caggtcgctt tcagcgacgc tgagaggtta aagaagtggc tttgtcgttt
     2641 cggtcgttac aaatgttaca attttccccg gaatcaaata ttctcacatg cgcgaacgcg
     2701 ccacatcggc gactgtgtca agttttacgt gaagccgcac atttgcacat ctatttcgta
     2761 ttttcttata tgcattatca atattcaatt aactttgcac cgctaaaaaa acttccgatg
     2821 tacgcattat tcaaaaagga aatcagttca tttctgcatt cgctgattgg ctatatagtc
     2881 atctgcgtct ttttggcagc caacgggcta attttgtggg tcttccccaa cagcttcaat
     2941 attcttggtt ttggttatgc cacgctcgac ggactattta ttctggcgcc ttttgtgttt
     3001 ctgtttctgc tacctgccat cagtatgcgc agctttgcag atgaaattaa cacaggcacc
     3061 atcgaattac tgatgaccaa acccatcagc gaaatgaaaa ttattgcagc caaatatctc
     3121 gcaggcatag tcattctcct tttttcactg ctcccaacac tgatttatct caacaccatt
     3181 tctcaactcg ctgttcccaa aggaaatctc gattttgggg gcatctgggg gtcatatatc
     3241 ggactgctct tccttggcgc ttcgtttctg gccatcggaa tttttgcatc atcgatatcg
     3301 aaaaaccaga ttgtctcttt tgtaattgcg gtttttctga ccgctttcat gtaccttgga
     3361 tttgaattca tttacgacag tggtattttt aaaggcgccg aactgtttat caaatcaatc
     3421 ggtatcaacg agcactatat ttcgatgagc cgcggtgtta ttgactcacg cgacctgctc
     3481 tattttctta gtctgattgc actttttctg atgttaaccc gcctttcact cgaacgtcgt
     3541 aaatggtaat aagtatggaa acgaaaacaa accacgggag caacagaaaa aaaagtctta
     3601 ttagactact ggctggaata gccatcctga ttgtgattaa tctggccgga acactgggct
     3661 tctttcgcct tgaccttact caggaaaaac gttatactct ttccgataac accatcgatc
     3721 tgctcaaaaa agtagatgat attgtatatt tccgcattta tcttgaaggg gaactgccgg
     3781 cttcatatac aaaactgcgc aatgccattc gcgaaaccct tgatgaattc agggcctaca
     3841 acaaagccaa catccaatac gaatttatcg accccaccga aggcaaggac aaacgcactt
     3901 tgaatgaata ttacaacgaa ttggtcgaaa aaggactgca gccatctgtc gatcgtcagc
     3961 aaagcagctc ttcgagcgag caaagaatca tatggccagg cgccctcgtt tcgtacaaaa
     4021 caagagaaac ggccatcaat tttattcaaa ccggacagca aaccgataag gaagtactca
     4081 ttaacgaatc gctcgaatca ctcgaattca atttgattga tgccatccgc cgattgtttg
     4141 tacgtaaaaa acccaatatt gcctttatag atggccacgg agaatcaagt gccatgatga
     4201 ccgaagacat cacccgaagt ctgaccgatt actacaacgt tcaacgagta acaatcaacc
     4261 agcagctcaa atcactcgat aaattcaaaa ccattataat tgccggacca gactcagttt
     4321 ttgatgaaaa agacaaattc atcatcgacc agtttgtgat ggatggaggg cgtgttcttt
     4381 ggctggttga cggaacagcc accaatatgg attcactgca gtcgagttct gaaacagtgg
     4441 ctgttgccaa tgaaatcaat ctggaagaca tgctgtttaa atatggtgtc cgcatcaaca
     4501 ataatctcgt gctcgacatg aatagttgtc ccattccggt aaaaaccggc cagattggcg
     4561 atcagccaca atttgaatat ttcaactggt tttacttccc cagtatttca aatactgacg
     4621 gacatccgat agtaaaaaac cttaatgcca ttcgttttga atttgtgtca ggcattgata
     4681 cagttggatc cgggggtatt aaaaagactg tgttgctcac ctcatcgcaa tacagcagaa
     4741 tagtaaacac cccggcagtt atcagtttac gcatgctgac cgaaactccc ggacgtgagt
     4801 ttttcaacaa acccaaaatt ccggtcgctg ttttacttga aggaaatttc agcagtgtgt
     4861 ttacaaaccg catcccacct gaaattgctg aagacaagga aattggattt tcggagtcag
     4921 gcaaaccatc caaaatgatt gttgtttcag atggcgatat catcaacaat cagtttgttt
     4981 accgcaacgg acaatacttc acttatccgc tcggctacga tcgtttcaca ggcatcaact
     5041 acggcaacaa ggattttctg ctcaactgca tgaattttct taccgatgaa tcgaacctgc
     5101 ttgatatacg ctcacgcgaa cttaaaatac gtctgctcga taaatctctg gtaacatcaa
     5161 atcaaaccta tattcagtgg tcgaatgtgc tggggccggt gctgattatt gtagccatcg
     5221 ggttaattct tatgatattc cgcaaaatca aacttaaaag aagcatatga aaaaatggct
     5281 gaaaaaatgg tatttctggc ttccattgct gctgattgtg gcagttttcc tgaccgtttt
     5341 cattaccaac cgttcaaaca cgacattgaa tccaggcgaa aagaattttt caatcactga
     5401 tacagcttcg gttacaaaag tttttcttgc cgacaaaaaa aacaattccg ttcttctcga
     5461 acgcaacgac agcaatggct ggatactgaa taaaaaatat tcggtgcaac ccgaaatgat
     5521 agaagaatta ttatacaccc tcatgtatat tactgtgcgg aatcccgttc ccaaaacaca
     5581 atacgatgct gtttacaagc aacttgcagg ttttgcaacc aaagttgaag tatatgcaaa
     5641 agtgtatcgg attgatttga tgggaattaa gctgtttcca tatgaaaaat gtatccgtac
     5701 atattatgtg ggctcagcca cgatggacaa cacaggatcg tacatgctga tggaaggcgc
     5761 cgaaatgcct ttcattatgc acattccagg ctttcgtggt tttctcgagc cacgctattc
     5821 accacgcgaa caagactggc gagaccataa agtgtttcgt tacaacctta cagacattgc
     5881 caaaatcaac gtaacatacc caaaatatgc cgacaggtcc tttgaattac tgaatccgga
     5941 taacatcaac ttttcggtta aaaacgaaat caacggcaaa ctggaaaata tcgatacggt
     6001 aaaagtgata aactacctaa acgcttatac tgatgcgcgt tttgaagagt ttctgacaaa
     6061 tcttccgatt caaaaacagg attctatccg aaagtctgca ccattttgca tcatcactgt
     6121 taccgaccgg aaaaatgaga ccaacagcat ttctttatat cggataaaac tgccggaggg
     6181 aagcaccaat atttcaggtg aacccattga atttgatccg gaacggctgt atggaatcgt
     6241 aaacggaaaa gatctcgttt cagcccaata ttttgtattc ggacccctga tgcgctatgc
     6301 aaacgaattt ttagactcgg atggcgcacc aacaaacaga tcaggtgaat ttgatacact
     6361 tttctgaaaa aacgtttttc tacattcgtt taattcatta attttgcacg aacaataata
     6421 ttaagctgct atgaatttca ttatttcgag caatattctt ctgaaaggac tgcaaaatgt
     6481 aagtggtctg ctttcaagca acagtctggt gcccattatt gaaaattttc tgttcagcac
     6541 cgacggaacc aaattgctgg taacagccac cgacaacgaa acccgtgccg tggtggaact
     6601 gagtcccgaa aaaatcaacg gcaccggcgc agtggccatt ccaccgcgtg ttttgcttga
     6661 gactctcaaa acttttccca atattcccat caccatcaat gtaaacgaag aaaccttcgc
     6721 cgttgaaatc agtgctggtg atggcgttta tcgtctggct ggctacaacc ctgaaaacta
     6781 tccatcaata gcagccatcg aaaatgcaaa atctatttcg atgagcagca tcgttcttca
     6841 aaatgcagtg agcagcacca tttttgcctg cggcaacgat gaaatccgtc cgcagctgat
     6901 gggtgtgttc ttcgagatca caccggacta caccaatttt gtggccaccg atgctcacaa
     6961 actcgttcgt ttccgccgca acgacatcaa atcggacgaa gcccattctt tcatcgttca
     7021 taaaaaaccg ctgaacctgc tccgaaacct gcttaatggt tttgattgca cagtttcagt
     7081 agaattcaac aataccaatg tttcattccg tttcgaaaac tatcaggttg tctgtcgcct
     7141 gctcgacggc aagtacccgg cttatgaagg tgtgattccg aaagacaaca attacaaact
     7201 ggtcatcaac cgcaattcgc tcatcagcac gttgaagcgt gtggctattt ttgcaaatca
     7261 ggcttcgcat caggttcgtt tcgatgttaa aggacaggaa ctcaatattt ctgctgaaga
     7321 cattgactat tcaaacgaag caaaagagcg tctgaactgc atttacgacg gagctgacat
     7381 tgaaatcggt ttcaattcga aattcttcct cgaaaccctt cagactctgg cctgcgaaaa
     7441 cattactgta acgctgtctt cacccaccag agccgccttg ctgattccgg aagaaaccaa
     7501 ttcggccgag gaggatgtac tcatgctcat catgcctatt atgctcaacg cataaaattg
     7561 caccttatat tctaaatgtc atcctcaggg atgacatttt ttttaaattc gcttcggcac
     7621 agtttttagt aaacctaaag tcatgaaaag attaaacaca ttttttgcat tcattctgct
     7681 cagcactata gttatggcgc agcaggataa acttcaggaa accctggcgg cggatactat
     7741 tttgtatcag atttatcatc atttgccaac tggctggacc atgagcatgg atgataccag
     7801 tattacaatt taccgaattg aaaaatatac acatgtagaa tcggactgca gcaccatgag
     7861 tctcgatagt ctggccgcat taccgaaaaa tgagacagct ctcattcatt ttctgtacga
     7921 aaacaagtgg gatgctgaaa gacttttctg gacgagagaa tcaaacgata gtctgaatat
     7981 gatacttgga tctcttcctc aacaaatggg catttcgcat ttgtatgacg ctgaaaaaag
     8041 cacaagatcg aaccgtgtct atactggaaa aaacaagact gaaaaagaaa aaatcaatgc
     8101 ctattacaaa agacgcgcac agcttgtaca acaaatcaca gccattccaa cattcaatac
     8161 cagtctgttt agtcttcgtc aacgcaaaca aacagctctt cagagacctg gtgtttgcat
     8221 ttatcctttt ggcgtatata aagaaatgct gggagtgtac attgtaataa tggattattg
     8281 caaaaatcct ttggatgaaa aataatacca aataaaaata taatacagtc aatcaaagca
     8341 atttcattga ttgtgttgct gactgatgcc cgagacctca tgattctgta aaaaaccaag
     8401 acctgccaca caagccgccg gccatgtgct gagtctgacg aagcatggca gattgtgtat
     8461 tatcaagcat tgtcataaga cacagttctt tatattcgac tcctccggag tcagccaaaa
     8521 tgattgaact gaaatacaaa tcactttggt ataattggat ttctgaataa aggagtcccc
     8581 ctttaaagaa tttgaagctt ttcaggtcaa aaaaaaaggt tattcaaatg aataaccttt
     8641 tttttcagaa ccgttaactc ttaacgacct ccgcgaaccg gtttaccagt gttggtagtt
     8701 acggtagcag tgttggttga aacagttgta gtgttggttt tcggtttgta agtaccggta
     8761 ccttgtttag cagcattggc aatgctgtct tcttttgctt tagcaattga atccaaacga
     8821 gcctgctcga gttcttcagc tttttggatg ctgtcttcag caacttttgc aagtgaatca
     8881 gctttgcgga ttgaatcctg ctttgctttt tcgtcatctt tcttgctacc accgcaagaa
     8941 accagcgcga acataccagc gagagctgcg attacaaaca gatttttcat gtttttttgg
     9001 tttagtggat aataatcaat aattacttaa cttcttcagc tttggctgaa tcagcattca
     9061 tggtgtcagc ggccagttct tcagcttttt tgatgctgtc tttcatgatg ctgtctttct
     9121 tcagtgaatc ctgttttgct ttgtcatcat ctttttttga tccaccgcat gaaacaagtg
     9181 cgaacatgcc aacgagggct acaattgcga ataagttttt cattcttttt tggttttttt
     9241 ggtttaacgg cacaaaagta atactttttt taatgatgta tcattttttt gtacttttgt
     9301 caaacaaata aagttgaaaa ctatgaaaaa aacattctta aaatttgcag ttgctattgc
     9361 agcagtagca ttcatcggtg tttcatgtac cgaagatgac atcacgaacc caactattac
     9421 catcactggc agcgcagctg ttaccattga ccttggtgac acctataccg atgcaggcgc
     9481 aaccgccaat gatgacaaag acggagatct gacttcacaa attactacta ctggtactgt
     9541 agatgtaaac aaagtaggca tttatgaaat cgaatatacc gtttctgacg aagctggcaa
     9601 cactacaacc gaaaccagaa ccgttactgt tcaggccaac cgtcttgcag gttcttacaa
     9661 tgtgagttgt gttattactg gtgcaggtgc cggaaattat aacttcgtag aagttgtaac
     9721 cggatcttca gtaaactaca actatattta ttttgctgat ttctgcggat tcccgggact
     9781 tcaggttcaa ggtactgtaa gtggcgctgg cattactttc aatcagacaa aaaattatga
     9841 ttgggatgat gatggtacag ctacagatgc tacaatcaca ggatcaacac tcagcgcttt
     9901 cactgtaacc gctggtgcta ccccaattgc agctctctca agtcttacct atgtaattga
     9961 ctatggtgca agccagatgg acaactgtac atcaacctac accaaacagt aatctgtttc
    10021 taaaaaaatt aaaaagagac tgtcctgccg gacagtctct ttttttttta tttttgaagc
    10081 atgaaacaga gcccgcagct gcgcctgcta atcagatacc ttatatactt ttcaggcgta
    10141 ctcctgctca tcactctggt gcgcctcctg ctttgggctt tcaataccca tttgtttaat
    10201 ccggcttcgg caaaaacctt ttttaatgta ctattcggtg gagggctttt tgatttcagc
    10261 actttttgtc tggtacatct gatatttatt ccttttcttc ttgtaccatt tgcatggctg
    10321 aataaaaaag tcttccggat tgggcgttac acgcttctgt tactggccgg gttcgttttg
    10381 ctggcgccta atatcatcga tgtaatctat tatcgcttca cgctgaaaag aaccactttt
    10441 gatgttttca gtttcatcgg gaccatggaa aacgaaatcg gcgggctttc cctgcgcttc
    10501 ctgattgatt tctggtacat gtttattgta tttattgtgc tgtttgttgg ctatatattt
    10561 ctgatcaaac gtacaatact gaataaaaag aaccgtgaaa ttgcggtaaa atacaaatac
    10621 caactgccgg ttgcgctttt gttttctgcc attttgatta tgggaggacg cggtagttta
    10681 gatatgcgac cgctgggaat tttacatgca tcgcgtgtgg ccggccctga atatgcgccg
    10741 cttgttgtga attcgactta taccattatt aagacatacg gtaaaaagga tctcgaaaaa
    10801 aaggactggt tcagcgaaat ggaactgccc ggaattttca atccggtaca ttattattat
    10861 tctgagacag actcaacaca gccactcaat gttgttatca ttatttccga aagtctgtcg
    10921 gccgaacact gcggttttct gaacggaggg ttaccctcat acacgccttt tctagactca
    10981 ctcagcaaac actgtctggt gttagaaaac atgtatgcca atggaaaacg aagtctggac
    11041 ggtattcccg ccatcatcag cagcttgccg ccactgatgc gcaatccttt tgtaacctcc
    11101 atgtattcgg gaaatgaaat tgaaggagtg ggcagtatgc ttggagacca gggataccag
    11161 acagcttttt ttcacggtgg atttaatggg accatgaatt ttgacggata cgccgccgcc
    11221 gccggatatg accaatactt cggcaaaagc gaatacccgg gtccggaaag cgattttgat
    11281 ggtcgctggg gcatttttga tgagcccttt ttacaatata tggcagaaaa atgcaacaac
    11341 tttaaagaac cttttacaag ctgcgtgttc acactatcag cacatcatcc ctacaccata
    11401 ccagatcagc acaaagggaa attttccaca ggcccgaaac ccatactcga aaccatcgga
    11461 tatgccgatt ttgcactcaa aaagtttttt gaaaccgccg ctaaaaccaa ctggtacaaa
    11521 aacactcttt ttatcattac agccgatcat accagcgagc tttattcgga atgttatacc
    11581 tcggttccat gcaatcacgc cataccaatg ctttggtatt atccgggcga cagcatgctc
    11641 aaaggccgat ctgatgtact ttgccagcaa gccgacattt tgccatccat tgcagactac
    11701 ctgaatctga atgataccat tattgctttt ggtaacagta ttttcgattc acaatcaaaa
    11761 cgctttgtaa ttatgcttaa tgatgtggtt tatcaaatgt tcacagatgg attgcaaaaa
    11821 tcggaattcg acggagaaac aaatttcaga caatcggatt acaaatgctg ttcaaaaaca
    11881 gagaacacta tcactacaaa cacagccaca tacaacaaaa gactgataaa agccatcata
    11941 cagcaattca acaacagaac catcagcaac cgattaaaaa agccatgatg catcggcaga
    12001 gtattatcat tattaatccc atagccggaa cacgacggat cgatggtgtc gaaaacctta
    12061 tccgaaactg gatcgatctt catcaaataa attgcaggat tgaaacgaca aaatatcggg
    12121 gacatgcccg ggagttagca tcagatgcag tttcagaagg attcaatcat tttattgttg
    12181 ccggaggcga cggaacgttg aatgaagttg cctcagccct gtacctgcaa cagaatgatg
    12241 ttactttggg aataatacca gtgggtagcg gcaacggatt agcgcttcac ttgcggcttc
    12301 cgaagaaaac cgaaaaagct ttggatattg cattcggaga aaaaacacta tcgtgcgatg
    12361 ttggtttatt taataatatt cctttcttca gcgttgccgg tgtaggtttc gatgccatgg
    12421 tcgcatacag gttttcgcaa atgaaaggac gcggacttcg gaattatatt cgtgcagtta
    12481 tgcaaaatta tccgggtttc aaaccatcgg attattgcgt tgaagccgag gggaaaaaaa
    12541 tcacaggcag atttctgatg atctcttttg ccaattcaaa tcagtttggc tacaacactt
    12601 cgctatcgcc tgaagccagt atttcagata gtttgattga tatttgcctg atgaaaaaag
    12661 ttccaattcc ggcagcagca cttctttcac ccttgctttt tttgaaaaag atgaatcaga
    12721 cccgttatct gaaaataata cggacaaaaa aagctcaaat cacagcttca ctgccagctt
    12781 attttcacat tgatggcgac ccatacatac ttgaaaaagc tgatatattg attgaaacac
    12841 tgcccgcagc attgaatatc catgtaaaac attgaatatg tccaaacaaa aaatttcagg
    12901 cttcgaagga ttgaaaaatc tggtgtacag caccagcagc gaaacgatga aagacattga
    12961 aaacaaccgt cagccgcagg aacccgatac tccggatccg ttacagcaaa atctgcgcgt
    13021 ttggattgaa aaaaatcacc ggggtggaaa agttgttact ataataaaag gatttgaagg
    13081 cagcgatcag gccttgaaag agttgggcaa aaaactgaaa aatcattgcg gtaccggtgg
    13141 cagcgagaaa gacggtgaaa tcatattgca gggtcaccag gccgacaaaa taatgaaata
    13201 tctggccgat aacggctata aagccaaacg cgccggaggc tgagcacaac gcttcgctct
    13261 gcgagttgaa agtgataaag ttgtaaagtt ataaaggtga cgtaaaaagg atgaggcatt
    13321 gctaaatgat taccgccaac cagtcaccgc tcactattta aaccatactt gctgattgct
    13381 gatatctgat tgctgaaagc tgacatgtac ttgcataaaa aaaagcctcg ctttcacagg
    13441 gctttatatt ttgatgatct atttggcagc aacatttgag agctgagcgg gctgcccaat
    13501 attacaacct tcgggctgag gcattcgcaa caaattaaaa tcgaatgtga ttacccttcc
    13561 atccacatcc aaatcctgag gtaacccttc aatgggaatc aatacctgtt cactctggtt
    13621 cacgaaaata actgttttac atccggtagc tctgaattta tgagacacag tcccaatggt
    13681 tgaacccata attactgtaa cattcgtatc gcttttcttt gcttccttat tgcatttaca
    13741 actgctgaga gcaaatgaaa aaactaaaag caataaaaca atccacttca tataatacac
    13801 tatttataat tgaattgtac aaatgtacat aattgtgcca ataatnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnataaa ataaaggact attcccggca attatttatg
    13981 accttctcat gcccctaaca acacttcttt acctaaaatg atgatattgc aaccaccaca
    14041 tatcatttat taattctgat ggcaaatcca agtgattttg ttgatgcaaa gttgaatgat
    14101 ttgttttgga aacagcgtta aataaaatac actaaatgtt gattactaat attttaaaac
    14161 atttattttt atacaaaagt gtgatttgta tttcacatct ttgtataaaa ataattttgg
    14221 tttatatgcc ttttgaaccc atctttaaaa atggaaatac cgactcattc attttcagga
    14281 aaataatcca gcaatcagca gtgcacggca acactgatga ccaaaatact tttgaccgac
    14341 tcgattaaag ggcgcttttt aaaatctcat tttattgaca atcaacatat ttgttgatga
    14401 tttcaaaaag cagacagata tgcatgcaca gaaatttcat tccgatagac atcgaaagcg
    14461 ctcaatatga caaaaatgga tttaaagaga tgtcgaaaaa tatctgaagc ttagaccaag
    14521 taccattggc ctgatataaa aaatcattgg cagcaaaata tcaatttgca aagaatcagg
    14581 aaagtatctt actgttctct tcagcttctt tgatcagttg ttgaaattct tcattggtaa
    14641 atccgctata gatataattg atgggattca ctttctgtcc gttacgaatc acttcgtaat
    14701 gtaaatgagg gccggtactg gggccggtat tgcctagcag gccaataatc tgacctcgtt
    14761 ttacagcttc gccttcttta acaagttttt ttgaaaggtg cgcatagaga gttctgaaac
    14821 cgtatccatg atcaataacg ataacaattc cgtaaccagt cattgatcca agcgggtcaa
    14881 taacaacgcc atcgccggtt gcataaatag gtgtaccaat cttaccggcc agatcaatgc
    14941 ctggatgaaa ttgcgatcct gctctggtaa aaggattgcg tcgccagcca aatccacttg
    15001 acagcttcac tctgttgcgg tcaacaggca taatgctggg tacagaagcc agcatttttt
    15061 ccttattttg aatcatgacc gacaaatcct gaaaagactt tgattgaaca acaatttttc
    15121 tacttatgag atcaagtttt gataaagttt ctttcagcaa gtggccattt tcaacaacat
    15181 tgaattgtga atacacagta gatgggccac ccactcccgg atttcgttcg tcttcaggaa
    15241 gcggatccat ggcaaaaata gttcgataag tccggtcgtc ttttgattga agagtattca
    15301 gaattgaaat cagtttatcg ctttttgaat tcagtaattc gagacgaaga cgataataat
    15361 taatatcgcg ggaaatccgg ttttcctggg ggctttcggt aaaaataaaa aaagcaatgg
    15421 caatggcaat tccaaccaaa actgaaaggc caagcccact caaaagcttt tttgtgagat
    15481 atttccccga tttttcgaca gaatgaagcg acaatgtaat cgggtcgata atgtattttg
    15541 ctcttttacc gaacataata caaaagtaca aagaatttca aaaacattga ataaatgcga
    15601 cttacagaaa catttaacag ctgtgaaatt aaaaaataca gcttaataaa accatgatgg
    15661 gtttcagtta atatgactga agaaataaaa tgccatcact cctgaagcaa tcagtttgag
    15721 caatacgccc gtcaaaaatc ccagaaaagt cccccaggcc gcacgaaatg cctgcttact
    15781 ctgtttccca atgatgaatt caccaattaa ggcgccaata aacggcatga ctatgatgcc
    15841 ccatggcggt aaaataaaaa tgccaagtat cagaccaatg gtactacccc agactccagc
    15901 cttcgaggcc ccaaactttt tagctccggc aacaggcaca acatagtcaa gcactgttac
    15961 aaatatcgca acaccagcca tgatcagaag aaatgtcaca ctaaatgggg gctcctcttt
    16021 aagctgtagc aacaacaacg ctgcaaaact caatggcggt ccgggtatta ttggtaaaaa
    16081 acaaccaagt attccaagca tggtgagtat tacacccagc gaaatccaaa gtatatcagt
    16141 ccatggaatg gatatcaata ttgcaagaaa ctccatgttt gagagtttta tgcaaaagta
    16201 aaaaaaaaaa tgatggagaa tcgctccaga atttttatac gaaagtgtga tttttatatc
    16261 acactttcgt ataattaaaa aatacatatt atattgatat acaacaactt tacatttaaa
    16321 ttatttgctt ttttggaatc taattcttac tacataatca ctgaaaatac aacatgaatc
    16381 cttgaacaga ataaaataaa aagtgaacaa attaaagaat cgcaactctc caaaataggg
    16441 taattttgaa aatacaacta tttcaaggtg cctttaatct ttcgattttt actaactttg
    16501 ctacaaattg atgtttatga agaaaattgt tcactccgaa aaagcgccca aagctgtggg
    16561 tccatacagc caggctgtac aaactggaaa tatgctcttt gtttccgggc agattcccat
    16621 ggatgcagaa accggaaaaa ttgttgaagg tggcattact gaacaaaccc atcaggtact
    16681 caaaaatatg ggtcatattc tccgcgaagc tggttacgac tacagcgacg tagttaaatg
    16741 cacagtctta ctcgataaca tggataactt cgccccgatg aatgaagtct atggacagta
    16801 ttttaccgaa aatgccccgg ctcgtgcagc ttatggtgta gtgcggcttc ctttgggtgt
    16861 tatggttgaa attgaatgta tcgctgcaaa ataaaagttg ctgtttatgt tgaattacat
    16921 tgtctggaat gttgacccca ttcttttccg cttgggatct cttgaagtgc gctattatgg
    16981 attgttattc gctctgggat ttcttgtcgg ttactacatt ataaaatatt ttttcaacaa
    17041 agaaaatgcg cctcaaatcg aaatcgacaa aatggcgctg tatatcattc ttggatctgt
    17101 tattggagcg cgtctcggac attgtttttt ctatgaaccc gatatatatc ttgctgatcc
    17161 cattcaaatt ctttttgtgt ggcagggtgg actcgccagt catggtggcg ccataggtct
    17221 tatttttgga ttatggctct attctttgaa atcctggaat cattcatggt tgcatatttt
    17281 agatcgaatt gccgtcccta cagcacttgc tggtagtttt atccgtctgg gaaacctttt
    17341 caacagcgaa atttacggct atcaaaccga tctgccttgg ggcttcgttt tcgtacgcga
    17401 cggtcaaaca atgccgcatc atccaactca gatttacgaa tcgctgggct atctcctcat
    17461 ttttattgtt ctgcttttta tgtataacaa gcgaaatgga ataataaacc gtggctttct
    17521 tgttggttgg gccattaccc tcatttttat tcttcgcttt tttgtcgagt tcattaaaaa
    17581 cgatcaggtt ggatttgaac aaaatatgat actcaatatg gggcaactcc taagcattcc
    17641 gtttatctta tgtggtattg ccctgattct gatatcaccc agacttggcc cggcacctga
    17701 atatatgccc gataaaggtg gcagtaagaa aaaagaaaaa tagttattgc attttcgcaa
    17761 tatgcacaaa gaaatcccgg ctgaaattca gtgtgggatt ttttgtttca aataaaccga
    17821 agcaagctga gccgctgccg ctcatggctg catatactgc acctgcattg tagagtttgc
    17881 tgatgattcc aacaatttca ggatattttt gcaaaattgg tttttcgaaa tcattgatta
    17941 gtctattgcg ccattcactg actggcagag aaatgacttc actcaccgga atccgcgcta
    18001 catcaggctt tacaagcgaa tacgcaacag gagtcgatat gcctgtttgt ccgtcggaca
    18061 ttttcaacgg cttgacaatt gcaattgcat aatttgataa atcgagttca atgcttttca
    18121 gtatttctcc cctaccctgt gccaatgcgg gttttgaatc aataaaaaaa gcacagtcgc
    18181 tgccgagttc ggccgcatag gttttcaaaa catcgtttga taattcaagc cggaaaaagt
    18241 tgttcagcag gacaaaagtc gcagcggcat ccgaagatcc gcctcccatg ccggctccgg
    18301 caggtatgct tttggcaaga tgaaaaataa ccggtttgat tttgtgccgg tcagccagca
    18361 atttccatgc tttaaagaca agattttgct caaccggcac aaacaggtct gttccataca
    18421 tgcggatatc cgttctggga gcggttttcg tgacttccag tgcatcgtgc agcgacacgg
    18481 gcaccatcac ggtttcaatg tcgtgatagc catcgggccg cttgcgcagc acctgcagac
    18541 cgagattgat tttggctttc ggaaaacaaa gcatgtacaa atatacaaaa atgggattga
    18601 aaaaagatgg acaaatttct caagccatga atgcaaaaaa gcagaccgaa gtctgctttt
    18661 tgtgcccgga acaggact
//
LOCUS       DAOPKE010000057        18495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3166_length_18495_cov_12.325488, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000057 DAOPKE010000000
VERSION     DAOPKE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3166_length_18495_cov_12.325488"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1527)
                     /locus_tag="PKY63_08385"
     CDS             complement(157..1527)
                     /locus_tag="PKY63_08385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPF00668.1"
                     /translation="MSENSTFNDAINYIMSALPMYQRVGKVAYKANLDNTIALLDYLD
                     NPQKKFQSIHIAGTNGKGSVSHCLASVFQESGYKTGLYTSPHLTDFRERIRINGIMIE
                     KSYVTAFIRKNKTFLDKLKPSFFEMSVGLCFQYFADRKVDVAIIETGMGGRLDSTNII
                     MPELSVITNIGFDHTAFLGNTLEKIAAEKAGIIKSGVPVVIGETQPAIKNIFINAAKK
                     NKSPIIFADAEIYAKTESHTLIPPSLTLRAAIEEDEHLIVSPLAGTYQIKNLKTVLAA
                     TITLRKTGYKLYIKNILKGITNVVQNTGLRGRWMVFDSKPVIVMDTGHNADGLSSISQ
                     MLKTLSYSTLHMVIGMVDDKDHNSMLQILPENAMYYFCKPSVPRGFDAKQLLKIAQQI
                     NIRGHAYKNVESAVNAAIKQATVNDVICIGGSTFTVADALTLKMFTPHKIKKFPPSGK
                     QIKMKL"
     gene            complement(1540..3864)
                     /locus_tag="PKY63_08390"
     CDS             complement(1540..3864)
                     /locus_tag="PKY63_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00669.1"
                     /translation="MKKIALLLFISALSISAFGQKGTIRGFVYDKGNSEPVIFTNVYL
                     KGTSYGSPTDINGYFTINQIPPGTYELMITYLGYDTLKETIVIKGNELLTKKYYLEKA
                     ARTINEVQIIGNRGDDTTQTQISKETVTPIEIKQVPTIGSPDIVQYITNIPGIITTGD
                     QGGQLYIRGGSSIQNKVLLDGMVVYNPFHSIGLFSVFETEIIRNVDVYTGGFGAEYGG
                     RISSVMDITTRDGNKKKYSGVFMANPFGANMVFEGPIVKDKGKGFNSSFVIAAKNSYL
                     SESSKIFYNYIDTAGLPFDFRDIYGKISLGTDNGSKINFFGFNFTDDVTYRSIANFAW
                     DANGGGANFMIIPGASTTIIEGVLAYSDYKLTLTDHTNLPKSSSINGFNIALTSTKFL
                     GKNSDIKFGLELIGFNTDLSFYNSARRQITQQDNTTEFAIFSKYKTIIDSSFIIEPSL
                     RFHAYPSLSESELEPRLALKYNITSKFRIKAAGGLYSQNLISASSDRDVVNLFYGFLS
                     GPENLQDQFDGKDVESRLQKAQHAIIGFEFGSFELSKDKTKQRTLTFNLEFYYKNFSQ
                     LTNMNRNKVYDDTGEYYDKPDYLKKDFIVENGNAEGVDLSAKYNSKHLYIWGVYSYGF
                     VHRYDGLIHYAPHYDRRHNANLVTSYRFGEFNKWEISFRWNLGSGFPFTQNAGFYPSL
                     DFTDGLGTDLFTSDESLGVLYDDYNAGRLPYYHRLDFSAKRTFYLTENTKLETNLSVT
                     NVYDRDNVFYFDRITNERVNQLPLMPSFGVTLRF"
     gene            complement(3894..5012)
                     /locus_tag="PKY63_08395"
     CDS             complement(3894..5012)
                     /locus_tag="PKY63_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPF00670.1"
                     /translation="MMTIKKVETRKDQKAFIMLPFELYKDNSYWIPPIIKDEYKIFSK
                     ATNPAMDFCDSCFWIAEENGKTIGRIAAIINHRYNEKVSEKLGRFSRLESINDGDVIG
                     ALLHTAEDWLREKGMTKVHGPLGFTNLDLQGLLVEGFDHLPSIASVYHHPYYQTHIEK
                     NGYEKENDWLEFRLKLEDHIPEKATRMVDLIKKRFNLKVISFKSKKEMLPYANKLFTL
                     LNSAFEELPYVSAFDEITKEFYIKKYFSFLNPEFVKVVVDEHNELRAFIVGLPSLSRA
                     MQKCNGKLFPFRFRHILHALKHPTEMDLLLTGVDPALQSQGVAAVLIFELQQVLLKYK
                     VPFVETTGIFESNNKAITTWKNYEHIQHKRRRCFVKNL"
     gene            complement(5009..6016)
                     /gene="holA"
                     /locus_tag="PKY63_08400"
     CDS             complement(5009..6016)
                     /gene="holA"
                     /locus_tag="PKY63_08400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPF00671.1"
                     /translation="MTHTDVIKEVNKKIFRPVYFLHGSEPYFIDVLSNHLGTSILDAS
                     EREFNQTVIYGRDANMSDLVSTLKCFPMMSNYQVAILKEAQDIKDKDFEQLTSYLANP
                     QPSSILVICYKKQAGKKLQNLFSKNPASVLIFESPEIKESALPDWIMKYLAQKKIVCS
                     PKTAQIIAGSLGKDLGKIANELDKVMLNIAEGGTLGMDEVEANIGISKKYNIFELQSA
                     IANNEKAKAMEIVSYFAANTRETSLLPIISILNSHFEKLFIYMQLCNSKSPADLATEM
                     GTRPYFLDQYRKSAGFFNMKKVLDVFKLLRDYDLKFKGIECVTPENELMREMIYKIVH
                     L"
     gene            complement(6013..6804)
                     /locus_tag="PKY63_08405"
     CDS             complement(6013..6804)
                     /locus_tag="PKY63_08405"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPF00672.1"
                     /translation="MKTKPEIVENWLPRYTGLSLDKFGEYILLTNFSQYVDLFAQIHN
                     VEIEGLDRSMPNATAENITIINFGMGSANAATIMDLLGAVNPKAVLFLGKCGGIKKIT
                     KVGDLILPIAAIRGEGTSNDYFPPEVPALPAFALQRVISSSVRDHHRDYWTGTVFTTN
                     RRVWEHDEEFKEYLIKVRAIAVDMETATLFSAGFFNHISTGALLLVSDEPMISEGVKT
                     EASDKKVNENYVEEHLNIGIGALQLLINNSVSVKHLLFDNDNPNR"
     gene            6901..7365
                     /locus_tag="PKY63_08410"
     CDS             6901..7365
                     /locus_tag="PKY63_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPF00673.1"
                     /translation="MNSNIALNLPLFNSRIQLVEGNEVIFDEFRKKWVRLTPEEWVRQ
                     QLLMYLHVHLGYPATAIAVEKSLTYNGMTRRPDAVISGRNGQILMVIECKKPDVTIDE
                     KTFLQAATYTSVLGNTYILITNGLSHFVCLADAASKKLDFLQEIPDYVTLNA"
     gene            complement(7452..8075)
                     /locus_tag="PKY63_08415"
     CDS             complement(7452..8075)
                     /locus_tag="PKY63_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPF00674.1"
                     /translation="MDEKHLHKHEKQTLLVVILTAVTMAVEIVFGLTTNSMALLADGI
                     HMGSHVLAIGLSWVAYILVRRVANTEKYKGKGDRILSLSGYTSGLLLLIFAFGIAWGA
                     IQRINNPVDIQFREAILVACIGLAVNILSALLLRHDHEEEKDHNLQAAYLHVIADALT
                     SVLAIGGLTAAMLWNIIWIDGACALAGAVIISRWSIKLIVQSGKSLI"
     gene            complement(8200..9606)
                     /locus_tag="PKY63_08420"
     CDS             complement(8200..9606)
                     /locus_tag="PKY63_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00675.1"
                     /translation="MIRLHFSALLFFAVCVTGHTQNDAPPAYVMLEEIVVTADDPRFA
                     PLFYSTSETGEGSLVYDLIDLVKTGKIEVGSYIVGKNKTDWNWIQQILGKREKDSWVF
                     NEIVDGKRFTGTLKKEITYSLKKVKGYRLFYITCYDEKFNLVTREFAGLTPLIPRPDD
                     SLNFKGYNVGYFSLKEPLVKAILEKKCTYSSFSSMSYLTFLRQIPVAELYPYAHFITP
                     YLADSLSDELQFKKPSASVFSYPEFVNGSACIVMKDLEKEKFQDSVFYIYANDFYNIS
                     NIYNSFQKHARSFGNIPFYKSEKDFGYTSIFDAIESTIYQCLDVYTYDSINLFTQRLD
                     SIQIFALASKIDSVEVFDPFTGEHWHEALIGNFECAVFRILELHDGSEIYPIAFCIGK
                     NIIDENWDVTGYKPILWIPFNQQFISIVDSYQAYLPGYPQQMSLWGYLLSGLYKGNII
                     SERPIDNAEWQTLREFLK"
     gene            complement(10115..13030)
                     /locus_tag="PKY63_08425"
     CDS             complement(10115..13030)
                     /locus_tag="PKY63_08425"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPF00676.1"
                     /translation="MRPIFTTLLFLITISIFAQNDFSKYVNPFIGTGGHGHTYPGAQY
                     PYGMMQLSPDTRKEGWDGCSGYHFSDSVILGFSHTHLSGTGVSDYSDLLIMPFADNAA
                     EAYPTLSKVKTASQFSHKYEDASPGYYEVLLAENDINVALTATARCGMHKYVFNRSGK
                     NKILIDLQYRDEVTYSKITFENDSTISGVRYSKAWATNQKFYFVIKLSQPVRHKFLYL
                     NDTLQTGMKTAIGTNLKAVLFLPDTTSAFLMKVGISATGIEDAQKNLNLEIPDWNIKK
                     VLNQATEAWNKELGRIEYSTNNQNDKIIFYSALYHCMIVPTVFTNVDNTYLGRDFEKH
                     TAEGFDYYTVFSLWDTYRGYHPLMSVIDRKRTRDYVLTFLEQFNQVGLLPVWEFAANE
                     TFCMIGNHSIPVISSAIMNDIIVNPDTQKLLLNAMVETANTPRGGIGVYKEFGYIPSN
                     RESESVSKTLEFAYDDWCIAQVARKLGDEQVYTEFIKRAQSYKNIFNPENGFHAPKAN
                     GTFLKPFDPKEVNFNYTEANGWQYNFYIPQDVSGYMDLLGDEKIFAAKLDSMFEADSQ
                     TTGRDQSDITGLIGQYAHGNEPSHHMAYLYAWTSQPWKTQLYCDSILQHLYFNAPDGL
                     SGNEDCGQMSAWYVLSALGFYPVNPADGNFVFGSPQHNEASIHLENGKTFRIKTVGQS
                     DKNKYIQQVTLNGIAYSKNYISYADILAGDELVFVMGPEHSSYGAKPEDRPNSEITDN
                     KIQPLPYATLLNGIVFSKKNVMSLHSATGWQIEYRILPDGEWKKYKKPLRIKKTTLVE
                     YRNAGEVETDMTQAIKKNIHLKVELFQPYNSQYTAGGPSGLVDGQRGGEDFRTGGWQG
                     YEGTDFTAVIDLGKKQMVRRITLGCLQEARSWIWMPTSVEYYYSVDGVNYIKLGSLGH
                     NVPDTDLGAYVMDFPLDFVPVEARYIKVFAKNYGVCPDWHLGKGGKAWIFVDEIIVE"
     gene            complement(13064..13234)
                     /locus_tag="PKY63_08430"
     CDS             complement(13064..13234)
                     /locus_tag="PKY63_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00677.1"
                     /translation="MNTEQGTPNFEVKSEIEKASLRIHKNSLLAAHHSLLTTHCSPLA
                     AHHSLLTKGAQP"
     gene            complement(13247..14194)
                     /locus_tag="PKY63_08435"
     CDS             complement(13247..14194)
                     /locus_tag="PKY63_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPF00678.1"
                     /translation="MSKNNLVCGIDIGGTNTTAGLVTPDGTIVLKKNIPTTTHKTIGD
                     FIDAVATLIQNMVSETGCALTGIGIGAPNGNFYNGTIEFAPNLPWKGVIRFTEQLKKY
                     FPTTNIILTNDANAAAWGEKIYGAAKGENDFIMITLGTGVGSGIVCGGNLVYGHDGFA
                     GEIGHTIVDPNGRPCGCGRKGCLETYTSASGVVKTALELLESAQQKSLLSKIPPEKIT
                     SKEIADAALKGDKLAIEVFDKTAYYLALKLSDAVAHTSPSSIYIFGGVAKAGDLLMLP
                     LKKYFNEFLLQIYKNKVELKWSALPDNDAAILGAASLVQ"
     gene            complement(14191..15621)
                     /locus_tag="PKY63_08440"
     CDS             complement(14191..15621)
                     /locus_tag="PKY63_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPF00679.1"
                     /translation="MNDISASKQKMIFALVTTLFFLWGFITCMNDILIPYLKKVFELN
                     YAQAMLVQLAFFGAYFIGSLLYFFISSAFDDPINKIGYRKGIVAGLLLSAIGCLLFYP
                     AANMHLYGFFLAALFVLGLGFTILQIAANPYVAILGSPETASSRLNLSQGFNSFGTTI
                     APIVGGYLVFHFFAQWGTPLLTNSGEQVLTEGGTTVSASSVQLPYLIFAGVFILMALV
                     FAFSHLPRIDAGNTGKESGSALKYRHLILGIIAIFFYVGSEVAIGSTIINYLNDLMGY
                     AEMEAKLFLAFYWGGAMIGRFIGAISLNDMPDKIKKILWMAVAGILGFVVIYSAGYIE
                     SGLDIQRVFWFLVLMAVNFGAFMLGRSKPARTLGIFCLFIISLLLLTVFSTGELAMWA
                     IIGIGLFNSIMWSNIFTLAIDKLGKHTSQGSSLLVMAILGGALLPPFLGIVADQIGLQ
                     MSYLILIIPYAYLAFYGFKGYKPRSI"
     gene            complement(15750..16454)
                     /locus_tag="PKY63_08445"
     CDS             complement(15750..16454)
                     /locus_tag="PKY63_08445"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF00680.1"
                     /translation="MSETNSIKSRILYEDNHIIVLQKLYGEISQGDKTGDLSLPDLLK
                     AYIKEKYHKPGEVFLGVVHRLDRPVGGVMVFARTSKALERLNESFREGKARKTYLAVT
                     CAKPAPEAAELKNNLERNEKLNKSFIVDTPSKFSKEAILRYHWLGSSDRYSLVEVELL
                     TGRHHQIRAQLAHIGCIIKGDLKYGAKRSNPDGNLSLLSWKLTFPHPTTKEILSFTAS
                     LPDVQPWIELSKYIGC"
     gene            complement(16665..17480)
                     /gene="panB"
                     /locus_tag="PKY63_08450"
     CDS             complement(16665..17480)
                     /gene="panB"
                     /locus_tag="PKY63_08450"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPF00681.1"
                     /translation="MTDFRTLPKITTHVLQEMKLKGEKIAMLTAYDFSMAKIIDKAGI
                     DIILVGDSASNVMAGNETTLPITLEQMIYHATSVVRGVDRALIVVDLPFGTYQGDSKE
                     ALQSAIRIMKEAGASAIKMEGGKEIIESVKRILSAGIPVMGHLGLTPQSIYKFGTYSV
                     RAQEDEEAKKLKDDALLLEKAGCFGIVLEKIPAKLAKEVSECLKIPTIGIGAGRSCDG
                     QVLVLHDMLGINQDFSPRFLRRYHNLFAEMTGAVTNYIHDVKTEDFPSEKEQY"
BASE COUNT         5113 a         3872 c         3758 g         5752 t
ORIGIN      
        1 ataactgtac aataggcggt tataaaatct gaaattttat agtgtcgaag tcaggcgaaa
       61 tatctccaga ttgcaacttg aaattttcag atatacagct accgcaagtc ttcggtttgc
      121 tggattcatg ttataattgc atttttttat ttgcaattat aatttcattt tgatttgttt
      181 ccctgacgga gggaattttt taattttgtg tggggtaaac attttcagtg tcaaagcgtc
      241 ggccacagta aaggtactac cgccgataca aattacatca tttactgtgg cctgttttat
      301 tgcggcattt actgctgatt caacattttt atatgcgtgg cctctgatat ttatctgttg
      361 agctattttt aatagttgtt ttgcatcaaa tcctctggga acgctgggtt tgcagaaata
      421 atacatggca ttttcaggaa gtatttgaag cattgagtta tggtctttat catcgaccat
      481 tccaataacc atatgcaggg tagagtagga aagtgtttta agcatttgcg atatactgct
      541 aagtccatct gcattatgtc cggtgtccat tacaataact ggttttgaat caaaaaccat
      601 ccagcggccg cgcagtcctg tattttgtac aacattggta attcccttaa gaatattttt
      661 tatatagagt ttatatccgg ttttccgaag agttattgta gcagcgagca ccgttttcag
      721 gtttttgatc tgataggttc cggccagggg cgatacaatc agatgttcat cttcttcaat
      781 ggctgcgcgt agtgtcagag agggtggaat cagagtatgg gattcagttt tagcatatat
      841 ttcagcatca gcaaaaatga tgggggattt gtttttttta gcagcattga taaaaatatt
      901 ttttattgcc ggctgtgttt cgcctataac aacgggtact ccggatttta taattccggc
      961 tttttcagct gcaatttttt caagagtatt tccaagaaac gccgtatgat caaaacctat
     1021 attggtaatt actgacagtt caggcataat gatgtttgta ctatcaagtc ggccacccat
     1081 accggtttca ataatagcca catcaacttt ccgatcagca aaatactgaa agcaaagtcc
     1141 gaccgacatt tcaaaaaacg agggttttag tttatcgaga aatgttttgt ttttgcgaat
     1201 aaatgcagtt acgtatgatt tttcaatcat aattccgttg atgcggatgc gttcgcgaaa
     1261 atctgtcaga tgtggtgagg tgtacaatcc ggttttataa ccggattcct gaaataccga
     1321 tgcaaggcag tgcgaaacgc ttcctttgcc attggttcct gctatatgaa tactctggaa
     1381 ttttttctgg ggattatcca ggtaatcaag caatgcgatt gtattgtcaa gattggcttt
     1441 gtatgcaact ttcccaactc gctgatacat tggcaaagca gacatgatgt aatttattgc
     1501 atcgttgaat gttgagtttt ccgacatttt aaatttgtat caaaagcgca gtgttacacc
     1561 aaaactgggc atcagtggaa gttgattaac tctttcgttt gtaatacggt cgaaatagaa
     1621 aacgttgtcg cgatcataca cattggtaac cgataaattg gtttccagtt tagtattttc
     1681 tgtcaggtaa aatgttcttt ttgcactgaa atcgagtctg tgataatacg gaagtcttcc
     1741 ggcattataa tcgtcataca aaacaccgag tgattcatct gaggtaaaaa ggtctgttcc
     1801 caggccatcg gtaaaatcaa gtgatggata gaagccggca ttttgtgtga atggaaatcc
     1861 tgagccaaga ttccagcgaa agctgatttc ccatttattg aactcaccaa aacggtatga
     1921 tgtaacaagg ttggcattgt gtcggcggtc gtagtggggt gcataatgta tcaatccatc
     1981 gtagcggtga acaaagccgt atgaatatac tccccagatg tataagtgtt tgctgttgta
     2041 ttttgccgat aaatcaaccc cttctgcatt accattctca acaataaaat cttttttcag
     2101 gtagtcgggt ttgtcataat actcgccggt gtcgtcatat actttattgc ggttcatatt
     2161 ggttagctga gaaaagtttt tgtaataaaa ttcaagattg aaggtgagag ttctttgttt
     2221 ggttttgtct tttgacaatt caaacgaacc aaattcaaat ccgattatgg catgctgtgc
     2281 tttttgcagt ctcgattcaa catcctttcc gtcaaattga tcctgcagat tttcgggccc
     2341 cgataaaaag ccgtaaaaca ggttaactac atcgcgatcc gatgaggcag aaataagatt
     2401 ttgtgaatat aatccgcccg cagcttttat tctgaattta ctggttatat tgtattttaa
     2461 tgccagtcgt ggttcaagct ctgattccga taagcttggg taggcatgaa atcgcaatga
     2521 tggttcaatg ataaagctgc tgtcgatgat ggttttatat tttgaaaaaa tggcaaattc
     2581 tgtggtgttg tcctgttgag ttatctgcct gcgtgcacta ttatagaatg aaaggtcggt
     2641 attgaatcca atcagttcga gtccgaattt aatgtcagag ttcttaccga gaaatttggt
     2701 agatgtcaga gctatattaa atccgttgat ggatgagctt ttgggaaggt tggtatgatc
     2761 tgtcagtgtc agtttatagt ctgaataggc cagcacaccc tcgatgattg ttgttgacgc
     2821 accgggtatt atcatgaagt ttgccccacc gccattggca tcccatgcga agttggctat
     2881 ggaacggtaa gtaacgtcat cggtgaaatt aaatccaaaa aaattgattt ttgatccgtt
     2941 atccgtaccc agactgattt ttccgtaaat atcccgaaaa tcaaatggca ggcctgctgt
     3001 atcgatgtag ttgtaaaata ttttcgagct ttcgctcagg tatgaattct ttgctgcaat
     3061 aacaaaagac gaattaaaac ctttgccttt gtcttttaca atcggccctt cgaataccat
     3121 atttgcaccg aaaggattgg ccataaaaac tccgctgtat tttttcttgt tcccgtcgcg
     3181 tgttgttata tccatcaccg atgatattcg ccctccgtat tctgctccga atcctccggt
     3241 gtaaacatct acattacgga tgatttctgt ttcaaatacc gaaaacaatc ctatactgtg
     3301 aaatggatta tagaccacca tgccatcaag cagcactttg ttttgtatgc tcgatccacc
     3361 gcggatatat agttgtccac cctgatctcc tgttgtgata atacccggga tattggtgat
     3421 gtattgtaca atgtcaggcg aaccaatggt gggcacctgt tttatttcga tgggtgttac
     3481 tgtttcttta ctaatctgcg tttgtgttgt atcgtctcca cgattcccaa tgatttgcac
     3541 ttcgttgatg gtccttgctg ctttttccag atagtatttc tttgtcagca gctcattgcc
     3601 tttgattaca attgtttctt tcagtgtatc gtatccaaga tacgtaatca tcaattcata
     3661 tgtccccgga ggaatctggt tgatcgtgaa atatccgttg atatctgttg gcgatccgta
     3721 gctggtcccc ttcagatata cattggtgaa aatgaccggc tcgctgttgc ctttgtcata
     3781 aacaaaacca cgtatggtcc ctttttgtcc gaatgcgctt atcgaaagtg ccgaaataaa
     3841 cagtaataat gctatctttt tcatgtgaaa tgcttggatg gtgttggttc gattcaaaga
     3901 ttttttacaa agcaacgacg tcttttgtgt tgtatgtgtt catagttttt ccatgtggtt
     3961 atggctttgt tgttgctctc aaatatgccg gtggtctcaa caaaaggaac tttgtatttc
     4021 aacaatacct gttgtagctc gaaaatgagt acggctgcaa caccttgact ctgtaatgca
     4081 ggatctacgc ctgtaagtaa taaatccatt tctgttggat gctttaatgc atgcagaata
     4141 tgacgaaaac ggaacggaaa taattttcca ttgcattttt gcatcgcacg cgaaagactg
     4201 ggcaatccaa cgataaatgc acgtaattcg ttgtgctcat ctacaaccac ttttacaaat
     4261 tcaggattta aaaaactgaa atattttttg atgtaaaact ctttggtgat ctcatcaaat
     4321 gccgaaacgt agggtaattc ttcaaatgct gaatttaata acgtaaaaag tttatttgca
     4381 tatggcagca tttccttttt gcttttaaaa cttattactt tgagattaaa tcgctttttt
     4441 atcagatcta ccatacgtgt ggccttttca ggtatatggt cttccagctt taatctgaat
     4501 tcaagccagt cgttctcttt ttcatatccg tttttttcaa tgtgcgtttg ataatacgga
     4561 tgatgataaa ccgatgcaat cgaaggcagg tgatcaaatc cttcaacaag caagccctgc
     4621 agatcgagat tggtgaatcc cagtggacca tgtacttttg tcatcccttt ttcccgcagc
     4681 cagtcttcag ctgtatgcaa taatgcgcca attacatcgc catcattgat gctttcaaga
     4741 cgagaaaaac gccccaactt ttcgcttacc ttttcattgt agcggtgatt gatgatggct
     4801 gctatccgac cgatggtctt tccgttttct tctgctatcc agaaacatga atcacagaaa
     4861 tccatggcgg gattggtggc tttgctgaat attttatatt cgtctttgat tattggggga
     4921 atccagtatg aattatcttt gtataattca aacggaagca tgataaatgc tttctgatct
     4981 ttacgggttt ccactttttt tatggtcatc atagatgtac tattttgtaa atcatttcac
     5041 gcatcagttc gttttccggc gttacacatt caatgccttt gaatttcaaa tcgtaatcgc
     5101 gcagcaattt gaaaacatca agcactttct tcatgttgaa aaatccagca gattttctgt
     5161 actgatcaag aaaatatgga cgggtgccca tttctgttgc cagatcagcc ggacttttcg
     5221 aattgcaaag ctgcatgtag ataaacagct tttcaaagtg actgttgaga attgaaatta
     5281 ttggcagcag tgatgtttca cgggtattgg cagcaaaata actaacgatt tccatggctt
     5341 tggctttctc gttgttggct atagcacttt gcaactcgaa aatattgtat tttttcgaaa
     5401 tgccaatatt tgcttccact tcatccatgc ctaatgtgcc gccttcggct atattcaaca
     5461 ttaccttgtc gagttcgttt gcaattttgc ccaggtcttt tcccaatgag cccgctataa
     5521 tttgcgcagt ctttggcgaa caaacaattt ttttctgtgc aagatatttc attatccagt
     5581 ccggtaatgc cgattctttt atttccggcg attcaaaaat gagtacagat gccggatttt
     5641 ttgaaaaaag attttgtagc tttttgcctg cctgtttttt gtaacaaatt accagaattg
     5701 agcttggttg cggatttgca agatatgatg ttaattgctc aaaatctttg tctttgatat
     5761 cctgcgcttc tttgagaatg gcaacctgat aattgctcat catcggaaag cattttaatg
     5821 ttgataccaa gtcactcatg tttgcatcgc ggccgtaaat cactgtttga ttgaattcac
     5881 gttccgatgc atcaagaatc gaggtgccca gatgattcga taaaacatca ataaaatagg
     5941 gctcagatcc atgcagaaaa tacacgggtc tgaagatttt tttattgact tcttttataa
     6001 cgtcggtatg cgtcatctgt ttggattgtc gttgtcgaat aaaaggtgtt tcaccgatac
     6061 gctgttgttt atcaaaagct gcaatgcgcc aatgcctata ttcaaatgtt cttcaacata
     6121 gttttcgttc acttttttat cgctcgcttc agttttcact ccttccgaaa tcatgggctc
     6181 gtcgcttacc agcaataagg cacctgttga aatatgattg aaaaaacctg ccgaaaataa
     6241 ggtggcggtt tccatatcta ccgcaatggc tctgactttg atgagatatt ccttaaattc
     6301 ctcgtcgtgt tcccaaacac ggcggtttgt cgtaaatact gtccctgtcc agtaatcacg
     6361 atgatgatca cgaacggaag atgatattac ccgttgcaat gcaaacgccg gcagtgcggg
     6421 aacttcaggc ggaaaataat cattcgatgt tccttcacca cgtatggctg caattggaag
     6481 aatcaaatcg ccaaccttcg tgattttttt tattccaccg cattttccaa gaaacaagac
     6541 cgcttttgga ttcacagctc caagcaaatc catgatggtg gccgcattgg cacttcccat
     6601 cccgaaattg ataatcgtaa tgttttcagc agttgcattc ggcatgctcc tgtcaagtcc
     6661 ttctatttct acattgtgta tctgcgcaaa cagatcaaca tattgactga agtttgtcaa
     6721 cagaatatat tctccgaatt tatccaatga aagcccggtg tagcgtggca accagttttc
     6781 tactatttcg ggttttgttt tcatgcgttt ctgtttttat tcaactcgtt cgctcttatt
     6841 tactaaattt gtaaacaggt ttcgaccaac aaaaataaca attttaaggt atatttttac
     6901 atgaattcaa atattgccct caatcttccg cttttcaatt cccgaataca gcttgttgag
     6961 ggcaacgagg ttatttttga cgaattccgc aaaaaatggg tgcgccttac tcccgaagaa
     7021 tgggtcagac agcagcttct gatgtatctt catgttcatc ttggctatcc cgccaccgcc
     7081 attgctgtcg aaaaatcact cacttacaat ggcatgactc gcagacccga tgctgtgatc
     7141 tcaggccgta acgggcagat tctgatggtt atcgaatgca agaaacccga tgtcaccatc
     7201 gatgaaaaaa ctttcctgca ggcggctact tatacctcgg ttttaggaaa tacttatata
     7261 ttgattacta atgggttgtc gcattttgtc tgtttggcag atgccgcctc caaaaaattg
     7321 gattttctgc aggaaattcc tgactatgtc acgctcaatg cgtgattttt cgttgaattg
     7381 aaaattagtt ttaggctgat taaaatcagt gttcggttgt aatattgata aaatattatg
     7441 ggtgttgatt tctatatcag actttttccg ctctgcacaa tcagtttgat tgaccagcgt
     7501 gaaatgatga ctgcacctgc aagagcacat gctccgtcaa tccagatgat attccaaagc
     7561 atggctgcag tcagaccgcc gatagccagt acactggtga gtgcatctgc aattacgtgt
     7621 aaatatgcgg cctgcaggtt atggtctttc tcctcttcat ggtcatggcg aagcaatagg
     7681 gcgctgagaa tattgactgc aagcccgatg caggccacca gaatcgcttc gcgaaactga
     7741 atatcaaccg ggttgttgat acgctgaatg gcaccccatg caattccgaa tgcaaaaatc
     7801 aaaagcagca gcccgctggt gtagcccgaa agcgaaagaa tacggtcgcc ttttcctttg
     7861 tatttttcag tgttggcaac cctgcgcaca agtatataag ccacccagct caacccaatg
     7921 gccagcacgt gcgatcccat atgaatacca tcggccagca gcgccattga attcgttgtc
     7981 agcccaaaca caatttccac tgccatggtt acggcagtca gaatcacaac caacagcgtc
     8041 tgcttctcgt gtttatgcag atgtttttca tccatgaatg caaatataca aaatcgctca
     8101 aagcacaatg cacaatgcac aacgcatatc tgatcgctat cgtatttcat attatcagcg
     8161 cattagcaca atatcacatc agcacattaa aaaccgtatt tatttcaaaa actctctcaa
     8221 agtctgccat tccgcattat caatgggacg ctcggaaata atattgcctt tgtacaaacc
     8281 gctgagcaga tagccccaaa gtgacatctg ctgaggatat ccgggcaggt aggcctggta
     8341 gctgtcgaca atgctgatga attgctggtt gaacggaatc cacaatattg gtttatatcc
     8401 agtaacatcc cagttttcat caataatgtt tttcccaata caaaatgcaa tcggatatat
     8461 ttcagagcca tcatgcaatt caaggattct aaaaactgcg cattcaaaat ttccaatcaa
     8521 tgcttcgtgc caatgctcac cagtaaatgg atcaaaaacc tcaactgaat caatctttga
     8581 agccaatgca aaaatttgta tgctgtctaa gcgctgtgta aaaagattga tggagtcata
     8641 tgtataaacg tccagacatt gatatattgt gctttctata gcatcgaaaa tcgatgtata
     8701 tccaaagtct ttttcacttt tataaaaagg aatattacca aaagaacggg catgtttttg
     8761 aaaagaatta tatatattgg aaatgttata aaaatcattt gcgtaaatat aaaaaaccga
     8821 gtcctgaaat ttttcttttt caagatcctt cattacaata catgccgaac cattgacaaa
     8881 ttcaggataa gaaaatacag atgcagatgg ctttttaaac tgtaattcat ccgacagtga
     8941 gtcagccaga taaggagtga taaaatgcgc gtatggatat aattccgcta caggtatctg
     9001 tctcagaaac gtaaggtatg acatggatga aaacgaacta tacgtgcatt ttttctccag
     9061 aatcgctttg accaatggtt cttttaaact gaaatatcca acattataac ctttgaaatt
     9121 cagcgagtca tctggccttg gaatcaatgg cgtcagcccg gcaaattcac gcgtgaccaa
     9181 attaaatttc tcatcataac aggtaatata aaacaaccga tatcccttaa cttttttaag
     9241 tgaataagta atttcttttt tcaaggtgcc tgtaaaacgc ttcccatcaa caatttcatt
     9301 gaaaacccaa ctgtctttct ctctttttcc aagaatttgt tgaatccaat tccagtcagt
     9361 tttatttttt ccaacaatat aactgcccac ttcaattttc ccggtcttta caaggtctat
     9421 taaatcatat acaaggcttc cttcgccggt ttccgaagtt gaataaaaca acggtgcaaa
     9481 gcgtggatcg tctgccgtga ccacaatttc ttcgagcatc acataagccg gtggagcatc
     9541 attctgagta tgacctgtta cgcaaacagc gaaaaataaa agcgctgaaa aatgtaacct
     9601 gatcatgtat tttatttttt caaatatata aactttttcg taatactgag ttgtatgcag
     9661 ctatcagctg acaactacaa atcactaaca acttcttaat tcctgcccct tacgccaaac
     9721 ctattttttc cacatccgat caccatcgga ttccaaatca tttcaagaat ctcatatttc
     9781 cacatcgccg taccggtcac agcttgccac gttccacgtg gtttcacgtg aggcgccaca
     9841 tcggcgtagc cgcacatatc cacattgtac tttgtatttc tgatttctga tttcgtattt
     9901 cacaacacct acttcctcgt ccccggagcc ctaaagggca tgcgggctat taatatttat
     9961 cgcctaccgg cgaatcatta tggaacttcg cgtgaaacgc gacaagtcgt agctttctct
    10021 gacgccttcg gcaactcaac atctcaacca ctcaaccttt caaccaaact cataactcat
    10081 cacccataac tcatcactca caattggcta ctgactactc aacaatgatt tcatcaacaa
    10141 agatccacgc ttttccgcct ttacccagat gccagtcggg acatactccg taattctttg
    10201 caaacacttt gatgtagcgc gcttcaacag gaacaaaatc aagcggaaaa tccatcacat
    10261 aagcgcctaa atcagtgtca ggaacattgt gtccgagcga tccaagtttg atataattca
    10321 ctccatctac agaataataa tattcaacag aagttggcat ccatatccag gagcgggctt
    10381 cctgaagaca gcccagcgtg atgcgacgta ccatttgttt ttttccaagg tcaatcacag
    10441 ccgtaaaatc cgtgccctca taaccttgcc agccaccggt acggaaatct tcgccaccgc
    10501 gctgcccgtc aacaagtccg ctgggtccac cggcggtgta ctgcgaattg tacggctgaa
    10561 ataattcgac tttcaggtga atgttctttt ttattgcctg tgtcatatcg gtttcaactt
    10621 cgcctgcatt gcggtattcc accaaagtcg tttttttgat gcgcaggggt tttttgtatt
    10681 ttttccattc accatcaggc agaatgcgat attcaatttg ccatcctgtt gctgaatgca
    10741 gcgacattac attctttttt gagaatacta ttccattcag caaagtggcg tagggcagcg
    10801 gttgtatttt gttatcagta atttcagagt tcggacggtc ttctggtttg gcgccatatg
    10861 agctgtgttc aggtcccata acaaaaacca gttcgtctcc tgcaagaata tctgcgtatg
    10921 aaatgtagtt tttactatac gctatcccat taagcgttac ctgctgtatg tatttgtttt
    10981 tatcgctttg tccgacagtc ttgatacgaa atgtttttcc gttttcaagg tgaatggatg
    11041 cttcgttgtg ttgcggagag ccgaatacaa agttcccatc ggcgggattg accggataaa
    11101 aaccaagggc gcttaaaacg taccatgccg acatctgccc gcagtcttcg tttccggata
    11161 acccgtccgg tgcattgaaa tataaatgtt gcagaattga atcgcaatac agttgtgttt
    11221 tccatggctg cgacgtccat gcgtacaaat aggccatgtg atgactgggt tcgtttccat
    11281 gcgcatattg tccgatcaaa cctgtaatat cgctctggtc gcgacctgtg gtttgtgagt
    11341 cggcttcgaa catcgagtcc agtttggccg caaatatttt ttcatcaccc agcaaatcca
    11401 tatatccgct cacatcttgc ggaatataaa agttgtattg ccagccgttg gcttcggtat
    11461 aattaaaatt gacttctttc ggatcaaaag gcttcagaaa agtaccattt gctttcggcg
    11521 catggaagcc gttttcagga ttgaaaatat ttttatagga ttgtgctcgc ttaatgaact
    11581 cagtgtaaac ctgctcgtca ccaagctttc gggctacctg tgcaatacac cagtcgtcat
    11641 acgcaaattc cagtgtcttc gaaacgctct ccgattcacg gtttgatgga atatatccaa
    11701 actctttata cactccgatt ccaccccttg gcgtatttgc ggtttctacc atggcattca
    11761 acaaaagttt ctgtgtgtcc ggattgacaa tgatatcatt catgatggcg gatgaaatta
    11821 ctggtatgct gtgattgcca atcatacaga aagtttcgtt ggcggcgaat tcccacacag
    11881 gcagcagacc gacctgatta aattgttcaa gaaatgtcag cacgtaatcc cgtgtgcgtt
    11941 ttcgatcgat gacgctcatc agcggatgat atccgcgata ggtgtcccag agcgagaata
    12001 cggtatagta atcaaatcct tccgctgtgt gtttttcaaa atcacgtcca agataggtat
    12061 tgtccacatt ggtaaaaaca gttggaacaa tcatgcaatg ataaagcgca gaatagaaaa
    12121 taattttatc gttttgattg tttgttgaat attcaatgcg tcccaattct ttgttccacg
    12181 cttcagttgc ctgattcagc actttcttga tattccagtc tggaatttca agattcagat
    12241 ttttttgtgc atcttcgatt ccggtcgccg atatgccaac tttcatcaga aatgcagatg
    12301 ttgtgtcggg aagaaacaaa acggctttca gattcgttcc aatagctgtt ttcatgccgg
    12361 tttgcaaggt gtcgttcaga taaagaaatt tatgacgtac tggttgtgaa agcttaatca
    12421 caaaataaaa tttctgattg gttgcccatg ctttactgta gcgcacacca ctgatagtgc
    12481 tatcgttttc gaaagtgatt ttcgaatagg ttacttcgtc gcggtattgc aaatcgatca
    12541 ggattttgtt tttcccgctg cgattgaaaa catatttgtg cattccgcaa cgggcagtgg
    12601 ctgtcagcgc cacattgata tcattttctg caagcagcac ttcataataa ccaggcgatg
    12661 catcttcgta tttgtgcgaa aattggctcg ctgtctttac ttttgaaagt gtaggatatg
    12721 cttcagctgc gttgtcagca aagggcatga tgagcagatc gctgtagtcc gaaacgccgg
    12781 tgccgctcaa atgagtgtgt gaaaaaccaa ggatgaccga atcgctgaaa tgatagcccg
    12841 aacagccgtc ccatccttct ttgcgtgtgt caggactcag ttgcatcatg ccataaggat
    12901 actgtgcacc gggataagtg tggccatggc cgccggttcc aatgaatgga ttgacatatt
    12961 tcgaaaaatc attttgcgca aaaatgctga tggtaataag aaaaagcagc gttgtgaata
    13021 ttggtctcat cagaatattt ttaggacaag gttaagaaca aggttaaggt tgagcacctt
    13081 tggtgagcag tgagtggtga gcagcgagcg gtgagcagtg agtggtgagc agcgagtggt
    13141 gagcagcgag cagtgaattt ttgtgaattc gcaaagaagc tttttctatt tctgacttca
    13201 cttcaaaatt cggtgttcct tgttcagtgt tcatgattca aagggtttat tgaacaagag
    13261 aagcggcgcc aagaatagca gcatcattat cgggcagggc cgaccatttc agttcgactt
    13321 tgtttttata aatttgcaac agaaattcat tgaaatattt tttcaaagga agcatgagca
    13381 aatcgccggc ttttgccaca cctccaaaaa tataaataga tgaagggctg gtgtgagcta
    13441 cagcatcgga tagttttaaa gcaaggtaat atgcagtttt atcaaaaact tcaatggcga
    13501 gtttatctcc tttcaatgct gcatcagcaa tttcctttga agtgattttt tctggtggaa
    13561 ttttgctgag aagcgatttt tgttgtgcgg attcaagaag ttcaagggcg gttttcacaa
    13621 cccctgatgc cgaagtatat gtttcgagac atcctttgcg gccgcatcca cagggacgtc
    13681 cgttgggatc aacaattgtg tgacctattt cgccggcaaa gccatcgtgg ccgtaaacca
    13741 gattcccgcc acatacaatg ccgctaccaa cgccggttcc aagagttatc atgatgaaat
    13801 cgttttcacc ttttgcggct ccgtaaattt tttcgcccca ggcagctgca ttggcatcat
    13861 tggtgagaat aatattggtt gtcgggaaat atttttttaa ttgttccgtg aaccggataa
    13921 cacctttcca tggcagattg ggagcgaatt cgatggttcc gttgtaaaaa tttccattgg
    13981 gggctccgat accgatgcct gtaagagcgc atccggtctc gcttaccatg ttttgtatca
    14041 atgttgctac agcatcgata aaatcaccaa ttgttttatg tgtggtagtt ggtatattct
    14101 tttttaaaac aattgttcca tcaggggtaa ccagtccggc tgtagtgttg gtgcctccaa
    14161 tatcgattcc gcaaactaaa ttatttttac tcatattgag cgtggtttat atcctttgaa
    14221 tccgtaaaaa gccaggtaag cgtaaggaat aatgagaatc agatatgaca tttgcaatcc
    14281 gatctgatcg gcaacaatac ctaaaaatgg cggcagcagc gctcctccga gaatggccat
    14341 tactagcagt gatgagccct gactggtgtg cttgcccaat ttgtcgatgg ccagcgtaaa
    14401 aatatttgac cacataatgg aattaaaaag tccgatgccg ataatggccc acatggcgag
    14461 ttctccggtg ctgaacacag tgagcagcag cagtgaaatt ataaacagac agaaaattcc
    14521 gagtgtgcgt gccggtttcg aacgtccgag catgaaagct ccgaaattaa cagccatgag
    14581 aacgaggaac caaaatacgc gctgaatatc gagtccgctt tcgatgtagc cggccgaata
    14641 aatgacaaca aatccgagaa tgccggccac agccatccat agaatttttt taattttatc
    14701 gggcatatcg ttgagcgaaa tagcaccgat gaaacgacca atcatggcgc caccccaata
    14761 aaaagcaagg aacaatttgg cttccatttc ggcataaccc atcaggtcgt tgagataatt
    14821 gataatggta cttccgatgg ctacttcgct gccgacataa aagaaaatgg caatgatgcc
    14881 taaaatcaga tgccggtatt tcagcgcgct tccgctttct tttcctgtgt tgcctgcatc
    14941 gatgcgcggt aaatgcgaga atgcaaatac cagcgccatc agaataaaaa caccggcaaa
    15001 aattaaataa ggcagctgta cactggatgc cgaaacagtt gtgccgcctt cggtgagtac
    15061 ctgctctccg ctgtttgtta gcaatggtgt tccccattgg gcaaagaaat gaaaaaccag
    15121 atatccgcct acgatgggag ctatcgtcgt tccgaaggaa ttaaatcctt gcgacagatt
    15181 caagcggctg gaagctgttt ccggacttcc gagaatggca acatatggat tggctgcaat
    15241 ctgcagaatg gtaaatccca gaccaagcac aaacaaggca gcgaggaaaa atccgtataa
    15301 atgcatgttg gcggccgggt agaaaagcaa acatccgatg gccgacagca gcaacccggc
    15361 aacgatgcct ttgcggtagc cgattttatt gattggatca tcaaatgccg atgaaataaa
    15421 gaaatacagc aacgagccaa tgaaatatgc cccaaaaaac gccaattgaa ccaacatggc
    15481 ttgcgcataa ttcaactcaa atactttttt aagatatggt atcagaatgt cgttcatgca
    15541 ggtaatgaat ccccataaaa agaaaagggt cgttaccaat gcaaaaatca ttttttgttt
    15601 cgatgcagaa atatcgttca tgcagtaaat tttgattgac aaaaatagaa tattaattgt
    15661 tgttcatgca tcagttttct caaaatgcca gatgaatatg acaaacgaca ctgattttga
    15721 taaacgggaa tgctaaattc ttcgaggtct tagcaaccaa tatattttga tagttcaatc
    15781 cagggttgca catcgggcag ggaggccgtg aacgaaagta tttctttggt agtcgggtga
    15841 gggaaggtga gtttccacga tagcagcgat aggttcccat ccggatttga acgtttggct
    15901 ccgtatttca gatcgccttt gatgatacag ccgatatggg ccagttgagc acgaatctga
    15961 tgatggcggc ctgttagtaa ttctacttca acaagtgaat aacgatcaga actacctagc
    16021 cagtgatatc gcagaatggc ttctttactg aatttcgaag gggtatcgac aataaatgat
    16081 ttattcagtt tttcgttgcg ttcaagattg tttttcagtt cggcggcttc gggtgcaggt
    16141 ttggcacatg ttactgccag ataggttttc cgtgctttgc cttcgcgaaa cgattcattt
    16201 agtcgttcaa gcgcttttga agtacgtgca aaaaccatca caccgcccac aggcctgtcc
    16261 aaccgatgca caacgcccag aaaaacttcg ccgggtttgt gatatttttc tttgatgtat
    16321 gctttcagta aatcaggcaa tgacaggtcg cccgttttgt cgccttgtga aatctcgcca
    16381 taaagcttct gcagaacaat tatatggttg tcttcataca gaatgcggga ttttatcgaa
    16441 ttggtttctg acattttttt cgttacgcgt tacgcgttac agacgttaca aggtgacaag
    16501 gtgacaaggt acattttcaa ttttttaact ttcagcattt taatccgttc gtatttcaaa
    16561 tttcgtattt ctgatttcgt attttatcgt cactcatacc tgtcccagct tgccgggttt
    16621 tacgttgttc cacgtggttt catgtgaagt cgcacatttt caaattaata ttgctctttt
    16681 tcgctcggaa aatcttcagt cttgacgtcg tgaatatagt ttgtgacagc acctgtcatt
    16741 tcagcaaaca aattgtggta gcgacgcaga aaacggggcg aaaaatcctg attgataccg
    16801 agcatatcgt gcagaaccag cacctgtcca tcgcaagagc ggccagctcc gatgccgatt
    16861 gtgggaattt taagacattc acttacttct tttgcaagtt ttgccggaat tttttctaaa
    16921 acgataccaa aacaacctgc tttttcgaga agcaaggcat cgtctttgag tttttttgct
    16981 tcttcgtctt cctgagcacg aacggaatat gtcccgaatt tatagatgga ttgcggggtg
    17041 aggcccaaat gccccatgac cggaatacct gctgataaaa tgcgttttac gctttcaata
    17101 atttctttgc cgccttccat tttgatggca ctggcaccag cttctttcat aatgcggatg
    17161 gccgattgaa gtgcttcctt cgaatctccc tgataagtcc caaatggcag gtcaacgaca
    17221 atgagtgcgc ggtcaacacc acgaacaacc gaagtggcat gataaatcat ttgctccagc
    17281 gtgatcggca gcgtggtttc atttccagcc attacgttcg atgctgagtc gcccacaaga
    17341 ataatatcaa ttcctgcttt gtctattatt ttggccattg aaaaatcata tgccgtcagc
    17401 atcgcaattt tttcaccttt cagcttcatt tcctgcagca cgtgtgtggt gattttcggt
    17461 agggttctga aatctgtcat ttcaaataaa tttggagttc aaagatagag ataataacaa
    17521 acatgtctgg tttgtaaatc agacttatat taaaatatta aaattcaaaa caaaattggt
    17581 agttatttgt gatgaaaaca cagataacta ctacaacaat tattaaaaac cggatctact
    17641 gatctctgct ggccttatct tttttcttgg ctgccttttc cagacgtttt tctttcagac
    17701 tttttgtcgg agcttttttc accgattttt ccttaagtgt tttttccttt gacatagtct
    17761 tgtatttttt agtagtacaa agataaaata taattgttca tatgtcaata agtcatacaa
    17821 taattatttt tgtgaaagca gagtcattgt acgtactttg acggataata ttacattgaa
    17881 accgttccgt accttacggc acgggcttgt tctatatctg gttctaccaa tattttgtcc
    17941 ctacgggata ataatgcagt atcatgtata tagtgccagc tcctgacctt ccaaagttta
    18001 aatgcaatgg aaaacctgta tgattcaaat gctgggttat tttctcaaat attctatgtt
    18061 ctccggatat tttctaccaa aacgattgat atatcggtgc aatcattttt ggacgactcc
    18121 ggaggagtca catatctgta gcccataaat acacaaaacc acgactccgg aggagtcaca
    18181 tataaaatgc ttcgtcttct gataatgcat gtcaatatgc gccctctctg gcatcaacca
    18241 acaaaatcat aagagcactt ctaaaaacca tttttgtcat attgagcccc gcgtttagcg
    18301 ggataaactc cgtcgaaata tcgctgcaat caattaatat tcagcttgtt aaaagcattt
    18361 caagacttat tgattgcaaa caaaatgagg ttttcagagg tacccataat gaatttgttc
    18421 cgattgacat tttatttttc atttagggcc tgctgaaaaa gtctgaggcg cgccagattt
    18481 gaggcgacaa gacga
//
LOCUS       DAOPKE010000058        17512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3401_length_17512_cov_13.258292, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000058 DAOPKE010000000
VERSION     DAOPKE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_17512_cov_13.258292"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1787
                     /locus_tag="PKY63_08455"
     CDS             426..1787
                     /locus_tag="PKY63_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPF00682.1"
                     /translation="MDPKVEQLGKGKISKLLLNYALPAIIATTASSLYNIIDRIFIGQ
                     GVGSMAISGLTLTLPIMNLAVAFGTLVGAGAAALVSIRMGQKRRADAIRILGNALTLN
                     IILGITFSVFALIYLDDILRLFGASDQTLPYAREFMQIILAANVIPHIFYGLNNVMRA
                     SGYPAKAMISILLTIAINIILAPIFIFVLDLGIRGAAMATVIAQTIGMIWVLAHFYSR
                     KPYIHFRFSGFKLRKRIVTDIFAIGMSPFAIHVAASVVSIITNLKLSEYGGDFAIGAY
                     GIIGSIGMLVVMIILGLTQGMQPIVGYNYGAGQYSRVTDVLKLTILWATAISFLGFLG
                     SMIFPGAIARAFTTDETMINTTVHGMRITMVVFFVVGFQVTVSSFFQYIGKAKIAIFL
                     SLSRQVIFLIPFLLILPLIFGLDGVWIALPTSDFIATITTAVILFYYRNKLRKQGETA
                     MAD"
     gene            2301..2750
                     /locus_tag="PKY63_08460"
     CDS             2301..2750
                     /locus_tag="PKY63_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPF00683.1"
                     /translation="MSLLEKISADIMAAMKAREMEKLEALRGVKAALLLANTAEGNKE
                     ITAEDELKILQKLVKQRKESAEIYKTQNRQDLYEVEINQAKVIETYLPAQMSEEEIKK
                     IIQDIIAQTGASSIRDMGKVMGMASKQLAGKADNSLVSRLVKEMLPA"
     gene            3017..5458
                     /locus_tag="PKY63_08465"
     CDS             3017..5458
                     /locus_tag="PKY63_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00684.1"
                     /translation="MKYKNIKEEELKNKVSQDYFWLYDCSRIIGNIDYCVCMIENQPS
                     LFDQESLLWAEAKRGSSDIYNSITQLILTIGKARTFDKHMPPPMLGAFDGEKIAFIPY
                     SDIHEIFYQNDFNWNVTPSDYNTKEFQLVHKRVKDTIDQKALLFHFEQDDKELIKFIR
                     HNFVVGKLGLTKTKIDKNNFMVIYNKWLRDVKPSIAVNWEAARKNGIIDGDFYLADLL
                     SDENNTLKDNLFVVLKATKYEFNRFKDEYGALTSSTVEFNDAQKAHAQFWNRYDRPPK
                     EEYWDYIIERRDLLVPQDIRERKGSFFTPQIWVELSQKYLTDVLGEDWQDEYYIWDCA
                     AGTGNLLNGLTNKYNIWASTLDKQDVEIIHDRIKNGTNLLEDHVFQFDFLNDDFSKLP
                     LPLQNIINDSKKRKKLVMYINPPYAEASDRATVVSKGENKTSVATEKRKYSEYNKLVG
                     TAARELFTQFFVRIHNDISGVKLASFSTLKYVNSQNFDKFRNYFKTEFQKGFVCPADS
                     FDNVKGKFPIGFIIWDLQTHKRISNIQCDVFDGDGIYVGKKSFFSVEKNKYIINWLRK
                     FYDKTNERIGYLRMQGTDMQNNQGVFFTNDPSKNDFVKHLLTNITKTNVMQMSIYLCI
                     RHCIDATWLNDRDQFLYPSNGWENDTVFQNDCFAFSLFYSQNKISGKNSINHWIPFTE
                     YEVGSREKFESDFMIQFIQGKGNPTMVQEALFDYPSPAKRNTPLEFSPEAQAVFNAGR
                     QLWKYYHKQPDCNVNASLYDIREYFQGRNEKGKMNNKSEDETYMNLISDLREPLKILA
                     KKIEPKVYEYGFLKQ"
     gene            complement(5464..5895)
                     /locus_tag="PKY63_08470"
     CDS             complement(5464..5895)
                     /locus_tag="PKY63_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF00685.1"
                     /translation="MKYCYEYPRPAVTVDAAVLVKKDDKLSILLIERKHDPYQGFYAL
                     PGGFIKKNETLEEAIARELQEETNLTLNNLQQFRAYSRPDRDPRGRTISVIFVSLLDA
                     FPAAVAGDDAAAVKWFPLNQLPELAFDHTEIVADIMKSMNK"
     gene            6201..8045
                     /locus_tag="PKY63_08475"
     CDS             6201..8045
                     /locus_tag="PKY63_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00686.1"
                     /translation="MRHFFWLFFLPVLSFAQQQTPDSSPAEWMNKNHEFSKSIFQIPE
                     KNIIMGDTLYITEDTLINGNWNFNGTLIAVDTGKILIQNATVLLLGDMLVFGNDARID
                     IINSTVNMPQQYFYQRSMIAAGNGIMNIKNSTLNFNGLSHNMSIADNGSVIMKDVTTT
                     GFRTCGLSGTGSLNIDGINQAGEFVITGSSNVAIKNAETVLLWHQFPAGSVITHSFPD
                     GDTLLSYDFNNTTPGVNGIDYNIEIDSCTDVMWGLMPEPNTDVTISNSLLRTIGLWFT
                     GSDSLNVSGLVNQSTYANQLMNISDRNLTLQACSVRTWSIYTFDTVKVNVQSCIVGEI
                     GTFGYSQLMTNTTMIDGSGGYFFAGDHSVCIGGYTTCSSAIRSQQNGIMLYVNSNVLY
                     GALEAQGTSVLIVAQSTIPQDPIHRDNGCAWFVNIEGPYEAYYGQPYSLHGSAWIDKD
                     PASPLMDFGSYQIFYSTDMGITWIAAGPEQNTEKRHEWLGDFNTALLPSDGSYLIKVT
                     LKDNQTTPVAIDAVVVLNVLPEYLSITEENDNIRVYPNPATENIFVEFPGPSDCTIKL
                     LSLDGRIILKKNTSSGNTIQMDVSGVKPGMYFIKTEGDIVSYRSIMIN"
     gene            8114..9292
                     /locus_tag="PKY63_08480"
     CDS             8114..9292
                     /locus_tag="PKY63_08480"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPF00687.1"
                     /translation="MKKIHVFILLFCTLTIAAQAQWTTMNSGTTDDLYAVRFSDEDNG
                     LVCGENGTILKTTNGGINWVIVPCSTTTYLNSIAYTSATTAYIAGYDGTVLKSTDSGN
                     SWISLNTGTFKHLTSVYFLDANTGFAAGESGEIIKTSDAGNTWTKINSGVSDYLHSIF
                     FTSPDTGYMTGNAGAIIKTTNAGTTWSALNSGTTVGIYSLYFTNALNGFAVTYDGNIL
                     KTSNAGTTWTSQSLGGFLTPLTSVYFTDENNGYAVGVVGTINHTNNGGASWVQQTSGS
                     VEVLQAVYFTNTIIGYAVGQGGTILKTINGGVTSVKDFSDEIINVYPNPASDAVYIGT
                     ENNIIYSVQMVTPNGQKVMEQNNIDEQKVQLNISDIKPGLYFLEIIGEQMYRKAIVVE
                     "
     gene            9430..10359
                     /locus_tag="PKY63_08485"
     CDS             9430..10359
                     /locus_tag="PKY63_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPF00688.1"
                     /translation="MNKKQLEAEYKNRIFSATDYIESHIDQSFTLEELSAVAAFSKFH
                     FNRIFKALIGESPFQYIQRIRLEKCAANMLSNPNDSISEIAFKWGFQDMSVFSRYFKN
                     YFGVTASEFKKQKSNIGQAESNMHQSACTNTGYFCNDKPEKQRRKTMKQNKSTEVKEF
                     PEMTVVYVRHIGPYKGNEELFSTLWDKLCTWAAARDLMQQRDLKFLTVYHDDPNITEE
                     NKLRISVCMTVPPDTKTDGEFGKMKIDAGKYVVARFDLGPGDFTEAWDWVYGSWLPTS
                     GYQPDDRPCFELYPEEPKDGRFIVDICLPVKPM"
     gene            10599..11042
                     /locus_tag="PKY63_08490"
     CDS             10599..11042
                     /locus_tag="PKY63_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HPF00689.1"
                     /translation="MKDSKARSIVKGISWRAVASLDTFVLGYIIFGNVAHASAIAGFE
                     VLTKIFLFFFHERLWNVVKAGRRPDGSVAPWRSLVKSISYRFWGSLDTTLLSYLVTGN
                     IGHSFILSGTEVVTKIGFFYLHERVWAHVKWGRIYEETDTKLCEV"
     gene            complement(11058..11621)
                     /locus_tag="PKY63_08495"
     CDS             complement(11058..11621)
                     /locus_tag="PKY63_08495"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPF00690.1"
                     /translation="MAAKNIQRCAWCIADPLYIKYHDEEWGIPVHDDYKMFEFMILET
                     FQAGLSWLTILRKRENFRKAFAGFDFEKVAKFTEKDFNRLMQDAGIIRNRLKIAAAIT
                     NAKAFIEVRKEFGTFCTYLWAFVDNKPVHSKLKLLADIPSSTPLSDAISKDLKKRGFK
                     FVGTTIVYAHLQACGLVNDHVKGCFRY"
     gene            11757..13499
                     /locus_tag="PKY63_08500"
     CDS             11757..13499
                     /locus_tag="PKY63_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPF00691.1"
                     /translation="MIKKILVCFVVALLALNTQAQDLPKGMTEEEKAMMPFYQFPQMR
                     SSITTPPTWTPRAMAEWEEIDALLITWTSYTSVLRQIVDAAQEECTVLIACDDSNAVK
                     SYLNSGGVPLTNVDYIEVPYTSVWIRDYGAHTIYQNVVGNHYLVDWEYNRPRPEDDLM
                     PEEHAAWAGLDLYSMTVSPWDFIATGGNLMVDGFGTAMSSELIVDENPSHSVAEIDTI
                     MKRFLGIDQYVLFPTLPYDGIHHIDMHMKLLDEETILVGQYPSGISDGPQIEANLQWL
                     LTNYMSCFGTPYKVIRIPMLPSEDGSTWPSSGAYYRTYTNGVFVNKTYIYPSYYTKYD
                     TTANRILTESLPGYTIVPIDCDAGGNSSPIAASGAIHCITNCIGSNDPLLISHQPLSS
                     PQTGSNSYQIDAYIKHESGIASANLYYKTSLVGSYVSVPMSSTGSDNWTGYIPVQGGG
                     TTVYYYIQAQASSGKSQVRPIVAPDGYWEFECQNSVGIEEPVAAIGMETIYPNPAAYI
                     TCIPVECGNGFTGRLSMMDATGRTVAVIHEGEFASGKNNYFFNAMAFESGLYIIQLEN
                     EESVQQQKVIVRNF"
     gene            complement(13575..>15577)
                     /locus_tag="PKY63_08505"
     CDS             complement(13575..>15577)
                     /locus_tag="PKY63_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00692.1"
                     /translation="WFQVGPFAADEMVEYRYPFIESNWIGNNNYDLAWGGNMFDVVAA
                     KDIVIDGFDLNLGTTTAASAKVYYRSGSYVGFESSNVGWILLDSVNLIQNGVDYHARL
                     DLTKSLTIAEGQTMGLYICTSVGIYYTTGNGTNETIVNDDMTCHFGNGGAFFSVIFSP
                     RVWNGRIYYKVAEEHKEDKSITSIFASNNQYNGNMFDVFAKNDIVIDSLDVNIISDGW
                     VKLYVRNGTVVGNNTSNAGWSLVDSVVVVASGQDIPTRVPLSNRILLRAGNLYGLYVC
                     SVSDMRYTGIVAGTNDVYTDSNMVLTGHYGGSNAFFCSSSRIWNGTLYYSVRNVDTVD
                     VSTQTSTSTYSSLMARGFYYVAPNDHVITGLRVPNEAAGTQYIEVVRLNNIPPSYSVT
                     TNEFVSLGRHTNVAGNAIVPVNAFIHKGDIIGVLGTRYSGTVTTMSWTANPTYPTTIL
                     GDSVTLYRMGTQTDFVNNPAQNLWTENSNWVGRVEMFVEPVTIHGGPDSVLIDVQTPA
                     PDLGADGEFCVQNTVTLDPGTFSSYDWSTSETTATIDVDTTGAPLNTPFDVWVTVTDQ
                     YGCTGSDTITITFIDCTGIDENDITLSIYPNPSDDVFYLRADNVQDEMLVQVFSLDGR
                     IVKEVVLNDTFGSIDMSNELPGVYSIRITIKDQVKEMRVVLQ"
     assembly_gap    15578..15677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15678..16438)
                     /locus_tag="PKY63_08510"
     CDS             complement(<15678..16438)
                     /locus_tag="PKY63_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00693.1"
                     /translation="MRTFLSVLMMVACVIVAAGQTIPFTESFENGLAKWNNLSTGGTV
                     EASTVDAIIGEKSLHITGNGVGEGLSLDFAASQNLSVLFYYVKVVGGDGGVVAAGNGH
                     SAINAAFYGEITGGNFVVTGSTGTTNLGPVVSNEWLRVEVKNMNFTDMTYDVYLNGVL
                     KGNGLPFRGSVSSISTLDVYNQTAGAEAWWDGIYAGVSNFDYPAFEDTKICANVVDEI
                     SVSPAVVLDSAGFYKDSTWFQVGPFAADEMVEYRYP"
     gene            complement(16569..>17512)
                     /locus_tag="PKY63_08515"
     CDS             complement(16569..>17512)
                     /locus_tag="PKY63_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00694.1"
                     /translation="IMNNSVEALKMYESPDGYFFTKRESGHPNEAAFNRRNKQYLHYY
                     KNQKFTSQDEVLGSFVGLALTIKFVDDEAIRKSARNQILRMINFMCGSRKTLWWRPLF
                     PDGTRMGNENGADGRAYAFALKTLAYKLVGKEYLKKHYPAIVRAPWCRDAYEGTELAC
                     LSGIGYSEYRILRFLMAEAMSVSGVSRVGNNVPKIIYLITDDYEWDTFYLLLHASLNN
                     IEVDGTLYDFNRLLTQLQQAPPNGTWQYGNSSYPKTPGWSAEFKWSATTAAQNGKTGE
                     SWALGNYSGIDFMLLHNLACLVIPEYNASWKNRFAKN"
BASE COUNT         5315 a         3771 c         3543 g         4783 t
ORIGIN      
        1 ttaaacaaat ggttgcggag aacttgctgc tctctctctc tctctacaaa tggccatttc
       61 cgggcgtttc aggaaattga ttttcataat tgatttgtaa ttggaactgc aataatacta
      121 aataaatttt attttgtcaa atatttttaa ttttttgtat gtattattca attttttaat
      181 gatgcaattt taatttatac agtcatataa ggattcttta ataacaaaat gacattaaag
      241 caagaataca acttccctcg aaaatatttc aataccgcaa aacattacac atttcaccgc
      301 gctccaggcc ccatgcattt agagttgcgc cttacaaaac tcaccacgga cctcccataa
      361 attatcgcga cacaactgac cactaattcc tatctttgcc cgccataaaa aaatatctgg
      421 gccggatgga tccaaaagtt gaacaattag gaaaaggaaa aatctcaaag ctgctgctca
      481 actatgcgct gccagccatt attgccacta ctgccagttc gttgtacaac atcatcgacc
      541 gcatatttat cggtcaggga gtcgggtcca tggccatttc ggggttaaca ctcacattgc
      601 cgattatgaa tcttgcggtt gcatttggaa cgctggtcgg agccggagca gctgcattgg
      661 tatccatccg tatgggacag aagcgaagag ccgatgccat caggatactg gggaatgcgc
      721 tgacactgaa tattattctc ggaatcactt tcagtgtgtt cgcactcatt tatctcgatg
      781 acattctgcg attgtttggt gcaagtgatc aaaccctgcc atacgcccgc gaattcatgc
      841 agatcatttt ggccgccaat gtcatcccgc atattttcta cggactcaac aatgtcatgc
      901 gggcttcggg ctatcctgca aaagcaatga tttccattct gctcaccatt gccatcaaca
      961 tcattcttgc accgattttc attttcgttc tggacctggg aataaggggt gcagccatgg
     1021 caacggtcat tgcacaaacc ataggcatga tctgggttct ggcgcatttc tacagcagaa
     1081 agccctatat acatttccgt ttttcgggat tcaaactcag aaagagaatt gttacagata
     1141 tatttgccat tggcatgtcg ccctttgcca ttcatgttgc ggccagtgtt gtttcaatca
     1201 ttaccaatct gaaactaagc gagtatggcg gagattttgc catcggtgca tatggaatca
     1261 ttggcagcat tggaatgttg gttgttatga ttattctggg actcacccaa ggaatgcagc
     1321 cgattgtcgg atataattac ggcgcagggc aatacagccg cgtgaccgat gttctgaaac
     1381 tgaccattct atgggcgact gcaataagtt ttcttggttt tctgggcagc atgattttcc
     1441 ccggagcgat cgcccgggcg ttcaccaccg atgaaaccat gatcaacact actgttcacg
     1501 gcatgcgaat aaccatggtt gttttctttg ttgttggatt tcaggtgact gtttcaagtt
     1561 tttttcaata catcggtaaa gcaaagattg ccattttcct gagtctttca cgtcaggtga
     1621 tttttctgat tccgtttctg ttgattttgc ccttgatttt cggactcgat ggtgtctgga
     1681 tagcgcttcc cacttccgat ttcattgcca caatcaccac cgctgtgatt ttgttttatt
     1741 acagaaacaa actgcgaaaa caaggtgaaa ctgcaatggc ggattaatac aattggaaca
     1801 ataaaagttt gtgtggaaat tcacccataa atcacatttc aagacgagct tataaaaaaa
     1861 ctctctgaca tccggaggct tgcccgacca acagaggcgg gatgtcaaac aataatagcg
     1921 tccggcgagc ttgtcatcac gtggaacgtg acaagctgtt ttacgtgaag ccggatgtga
     1981 taaagcccaa aaacaagacc ctgccagagt ctaattaata tgtgtttgaa aattattgat
     2041 gcaataaatt atccttgatt ggacctctcc gaggtcaata atattctatc tttttcacag
     2101 ccttgcaggc cgcgctatta ttgtttgacc tgcgctgcag gtcggacaaa atctgtcaca
     2161 tcagtgtctt cagcaggaga ccaggtttaa atgctggcaa aatcttcaga ctgcaagttt
     2221 cacacaaaaa caggtataac catacaattc aaattatata aattcggaat ttcgtatttt
     2281 tgtaaaaaaa caaaacaatc atgagtttac tcgaaaaaat aagtgctgat attatggccg
     2341 ccatgaaggc gcgcgaaatg gaaaaactgg aagctttgcg cggagtgaaa gctgcgttac
     2401 tattggccaa tacagccgaa ggaaataaag aaatcacagc cgaagacgaa ttgaaaattt
     2461 tgcagaagct tgtgaaacag cgcaaggaat cggcagaaat atataagact caaaaccgac
     2521 aggacctgta tgaggttgaa atcaatcagg ccaaagtgat tgaaacatat cttcctgctc
     2581 agatgagcga agaggaaatc aaaaaaatca ttcaggatat tattgctcaa accggcgcca
     2641 gcagtatcag agacatgggc aaggtaatgg ggatggcctc caaacaactg gccgggaaag
     2701 ccgataacag tctggtttcg cgattggtta aggagatgct gcccgcctga atcatcagtt
     2761 tgtaaagttt ataaagtttg taaagttgaa aggcgctttt gtagtagcat tctttaatgc
     2821 atatttaata tttgatgctg ctttcggttt tgtctgtaca atttctatcc ctgaaagtat
     2881 ttgcctcttg aaaagggcaa atgctttatt gctgcatcaa caaaagactt cggtgaattg
     2941 ttgataaaat ttcctgtaag gtctcagatt cttttgtaat tttatttggt caatagtatc
     3001 aggaattaaa aaaaacatga aatataaaaa cataaaagaa gaagaactta aaaacaaagt
     3061 ctctcaggat tatttctggc tctacgactg ttccagaata atcggaaata ttgactattg
     3121 tgtttgcatg atagaaaatc agccttcatt atttgatcag gaatcacttt tatgggctga
     3181 agcaaaaaga ggatcgtccg atatctacaa ttcaatcaca cagcttattc taaccattgg
     3241 taaagcacga acctttgata aacacatgcc accgcccatg cttggcgctt ttgacggaga
     3301 aaaaatcgcc tttattccgt acagtgatat tcatgaaatt ttctatcaga acgatttcaa
     3361 ctggaatgta actccatccg attataacac aaaagaattt cagctggttc acaaaagagt
     3421 caaagacacc atcgaccaga aagcactgtt gtttcatttt gagcaagatg ataaagagct
     3481 tattaaattc atacggcata attttgtagt tggcaaactt ggactaacaa aaaccaaaat
     3541 tgacaaaaac aatttcatgg tgatttacaa caaatggttg cgtgatgtaa aaccatccat
     3601 tgctgtaaac tgggaagcag cgcgtaaaaa cggaatcatt gatggcgact tttatctggc
     3661 cgatttatta tcagatgaaa acaatacttt aaaagacaat ctgtttgttg ttttaaaagc
     3721 taccaaatat gagttcaacc gtttcaaaga tgagtatggg gctttgacat caagtacagt
     3781 tgaattcaat gacgcacaaa aagcacacgc tcaattctgg aaccgctatg accggccgcc
     3841 caaagaagaa tactgggact atattattga acgccgggat ctgctggttc ctcaggatat
     3901 tcgtgaacga aaaggcagtt ttttcactcc gcagatatgg gtagagctgt ctcagaaata
     3961 tctgaccgac gtattagggg aagactggca ggacgaatat tacatatggg attgcgctgc
     4021 aggaacgggc aacctgttga acggactcac caacaaatac aacatctggg catcgacact
     4081 cgataaacag gatgtagaaa ttatccacga tcgtattaaa aacggcacca atttgcttga
     4141 ggatcacgta tttcagtttg attttctgaa tgacgatttt tcgaaactcc cattgccact
     4201 gcaaaacatt attaatgatt ctaaaaagcg gaaaaagctg gtgatgtata ttaatccgcc
     4261 gtatgcggag gcaagtgata gagcaacagt tgtttcaaaa ggcgaaaaca aaacttcagt
     4321 tgctacagaa aaaaggaaat attcagaata taacaaattg gtaggtacag ccgcaagaga
     4381 acttttcact cagttttttg tgagaattca taatgacatt tcaggagtca aacttgcttc
     4441 atttagcaca ttgaaatatg taaattctca aaacttcgac aaattcagaa actatttcaa
     4501 aacagaattt caaaaaggat ttgtttgccc agctgatagc tttgacaacg ttaaaggcaa
     4561 atttcctata ggttttataa tctgggattt acagacacac aaaagaattt ctaatattca
     4621 atgtgatgta tttgatggtg atggaattta tgtaggaaaa aagagttttt tcagtgtgga
     4681 aaaaaacaaa tatataataa actggttaag gaagttttac gacaaaacaa atgaaagaat
     4741 tggctattta aggatgcagg gcacagacat gcaaaataat cagggtgtat tttttaccaa
     4801 tgatccatcc aaaaatgatt ttgtaaaaca tttacttact aatataacaa agaccaatgt
     4861 aatgcagatg agtatatatt tatgtatccg gcattgtatt gatgcaacct ggctgaatga
     4921 ccgagatcag tttttatacc ccagcaatgg ctgggaaaat gacacagtat tccaaaacga
     4981 ttgttttgct ttttcgcttt tttatagtca aaataaaatt tccgggaaaa acagcattaa
     5041 ccattggatt cctttcacag aatacgaagt tggttcgcgt gaaaaatttg aaagtgattt
     5101 catgatccag tttattcagg gaaaaggtaa cccaactatg gttcaggaag cactatttga
     5161 ctacccatca cctgcaaagc gcaatacacc tttggaattt tcacccgaag cacaggcagt
     5221 gttcaatgcc ggacgtcaac tttggaaata ctatcacaaa cagcccgatt gcaatgtaaa
     5281 tgcttcgctc tacgatatcc gtgaatattt tcagggcaga aacgagaaag gtaaaatgaa
     5341 taataaaagc gaggatgaaa cctacatgaa tctaatttca gatttaagag agccactaaa
     5401 aattcttgca aagaaaattg aaccaaaagt atatgaatat ggttttttga aacaataaga
     5461 gtttcactta ttcatcgatt tcattatatc cgcaacaatc tcagtatgat caaaagccaa
     5521 ttccggcagc tgattgagcg ggaaccattt tacggcagcg gcatcgtcgc cggcaacagc
     5581 agctggaaat gcatcgagaa gcgaaacaaa tatcaccgaa atcgttcgtc cgcgcgggtc
     5641 ccggtcgggg cggctgtaag cgcggaattg ctgcaggttg tttaatgtca gatttgtttc
     5701 ttcctgaagc tcgcgcgcaa tggcttcttc gagcgtttcg ttcttcttaa taaatccgcc
     5761 aggcaatgca taaaatccct gatacggatc gtgtttgcgc tcgatgagaa gtatcgataa
     5821 tttatcatct ttctttacca gtacagctgc atcgacagtt acagccggtc gcgggtattc
     5881 ataacagtat ttcataaaac aaacatacaa aaactttttg ctatcttcgt ttttcgttta
     5941 cacacaaaat aatgaaagga aatctcttga gtctgttcta ctgttcatcc ttcgatggag
     6001 tttaccccgc tgtcgacatt ccgacgaagt ttattctgcg ggacgcaggg ctcaatgtga
     6061 cagcggcggg gctcaggatg acagatgcaa ggctcagagt gacagcaggc tgtgcttaat
     6121 aaattaaatt ttcaaagatc tccaaaaccg cataggggta aattcatttg aatgtgtaat
     6181 actgttgaaa acccaaaact atgagacact ttttctggtt attttttctt ccggttttat
     6241 cttttgcgca acagcaaacg ccggattcat caccggctga atggatgaat aaaaatcatg
     6301 aattcagcaa aagcattttt cagattcctg aaaaaaacat tattatgggt gacactttgt
     6361 acatcaccga agatacgctt ataaacggca actggaactt caacgggaca ctcattgcgg
     6421 ttgacacagg aaaaatattg atacaaaatg ccaccgtact tttattgggc gacatgcttg
     6481 tattcggcaa tgatgcccgc atcgacatca tcaactcaac ggtgaatatg ccgcagcaat
     6541 atttctatca gcgctctatg attgcggccg gaaacggtat tatgaacatt aaaaacagca
     6601 cgctcaactt caacggactg tcgcataaca tgtccattgc cgacaacgga agcgtgatta
     6661 tgaaagacgt cactactacc ggattccgca cctgtggcct gagtggaaca ggttcgctga
     6721 acattgatgg catcaatcag gcgggagaat ttgtgattac agggtcttcg aatgttgcaa
     6781 taaaaaatgc cgaaaccgtt ctgctctggc accagttccc ggctggaagc gtcatcactc
     6841 attcatttcc cgatggtgat actttgctga gttacgattt caacaacact acacccggtg
     6901 ttaacggcat tgattacaac attgaaattg attcatgcac cgacgtgatg tggggcctga
     6961 tgccggaacc caataccgat gtcaccatca gtaacagtct gctccgcacc ataggattat
     7021 ggttcaccgg cagcgattca ctcaatgtgt ccggattagt caatcagtca acctatgcca
     7081 atcagttgat gaacatcagc gaccgcaacc ttacactgca ggcctgctcg gtacgcacct
     7141 ggagcattta tacgttcgac acggtaaaag tcaatgtgca atcatgcatt gtgggcgaaa
     7201 tcggcacctt cggctattcg cagctgatga ccaacaccac gatgattgac ggcagcggcg
     7261 gatatttctt tgccggcgat cacagcgtat gcatcggagg ctacactaca tgcagttctg
     7321 cgatacgctc gcagcaaaac ggcattatgc tgtatgttaa tagcaatgta ctgtacggtg
     7381 cgctggaagc gcagggaaca agtgtgctga ttgtagctca gagtaccatt ccgcaagatc
     7441 cgatacaccg cgacaatggc tgcgcctggt ttgtaaacat cgaaggacca tacgaagcgt
     7501 attacggcca gccatactcc ttgcatggct cggcctggat tgacaaagac ccggcttcgc
     7561 cattgatgga tttcggatcg taccagattt tttacagtac cgatatgggc atcacatgga
     7621 ttgccgcagg gccggaacaa aacacggaga aacgtcacga atggctcggc gacttcaaca
     7681 cagcgttatt gccatccgat ggaagttatc tgatcaaagt cacattgaag gacaatcaga
     7741 caacaccagt tgccattgat gcggttgtgg tattgaatgt gttgcctgaa tatctgagta
     7801 ttacagaaga gaacgacaac atccgggtgt atccaaatcc cgctacagaa aacattttcg
     7861 ttgaatttcc cggaccatca gattgcacaa taaaactact ttcgcttgac ggcagaatca
     7921 ttctgaaaaa aaacacaagt tccgggaaca ccatccaaat ggatgtttct ggagttaaac
     7981 ccggaatgta ttttataaaa acggaaggag acattgtttc ataccggtca atcatgatca
     8041 actgaagtgt taaataatca ttgaattaat atattatgca tatatttgaa atcactcatc
     8101 aagcttcgct aaaatgaaaa aaattcatgt ttttattctg cttttctgca cattgactat
     8161 tgctgctcaa gcccaatgga caacaatgaa ttcaggtaca accgatgatt tatatgccgt
     8221 tcggttttcg gatgaagaca atggattggt atgcggagaa aatggaacaa ttttaaaaac
     8281 gaccaatggc ggcataaact gggtcattgt tccatgtagc accacaactt atcttaattc
     8341 aattgcatac accagtgcaa caaccgccta cattgccgga tatgatggta ctgtattaaa
     8401 atcaaccgat agtggaaatt catggataag tctgaacaca ggcacattta agcatttgac
     8461 atcggtatat ttcttagatg caaacacagg atttgcagca ggcgaaagtg gagagataat
     8521 aaaaacaagt gatgccggaa acacatggac aaaaataaac agcggagtta gcgattatct
     8581 gcattccata ttttttacaa gtcctgacac cggctacatg acagggaacg caggcgctat
     8641 cataaaaacc accaatgctg gcacgacctg gtctgcctta aacagtggaa caaccgttgg
     8701 gatttattcg ttgtatttta caaatgcctt aaatggtttt gccgttacgt acgatggcaa
     8761 catattgaaa acaagcaacg ccggtacaac atggacttca cagagcttag gtggttttct
     8821 cacaccatta acttcagtgt atttcaccga tgaaaacaat gggtatgcag ttggcgttgt
     8881 tggcaccatc aaccacacca ataatggcgg agcatcgtgg gttcaacaaa caagcggatc
     8941 cgttgaagta ctgcaggccg tatattttac aaacaccatt attggctatg ctgtaggtca
     9001 aggtggaacc attctgaaaa caatcaatgg tggcgttacc agcgtaaaag atttttctga
     9061 tgaaataatc aatgtatatc caaatcctgc atcagatgcg gtttacattg gcacagagaa
     9121 caacatcatt tattccgtcc aaatggtgac gccgaacgga caaaaagtga tggagcaaaa
     9181 caatatcgat gaacaaaaag ttcaattgaa tatttcagat ataaaaccag gtttatattt
     9241 tcttgaaata atcggtgaac agatgtatcg taaagcgatt gttgtggaat aaagaattct
     9301 gcagatgagt gattttatgc ggctttcgcg ctgcattcaa catgttttta tacagtatat
     9361 tcattttctg aaattgtgta tttttgaaaa cgatttcaaa tgttctgaat cagttttctt
     9421 tttgcaggca tgaataaaaa acaactggag gccgaatata aaaaccggat tttcagtgcg
     9481 accgattata ttgaatcgca catcgatcaa tcatttacat tagaggaatt atcggcagta
     9541 gcagcctttt caaaatttca tttcaaccgg attttcaaag cgctgatcgg cgaaagtcct
     9601 tttcaatata ttcaacgcat tcggcttgaa aaatgtgccg ccaacatgtt gtcaaatccc
     9661 aacgacagca tttccgaaat cgcgttcaaa tggggctttc aggacatgtc ggttttttcg
     9721 cgatacttta aaaattattt cggcgtaacg gcctccgaat tcaaaaaaca aaaaagcaat
     9781 atcggtcaag cggaaagcaa tatgcatcaa tccgcttgca caaacaccgg gtacttttgt
     9841 aatgacaaac ctgaaaaaca aaggaggaaa acaatgaaac aaaacaaaag taccgaggta
     9901 aaagaatttc cggaaatgac ggttgtttat gtccgtcaca tcgggcctta caaaggcaac
     9961 gaagaattat tttcaacctt gtgggacaag ctctgtacct gggctgcagc gcgcgatctg
    10021 atgcagcagc gcgatctgaa atttctgacg gtctatcacg atgatccgaa cattaccgaa
    10081 gaaaacaagc tccgcatcag cgtgtgcatg acggttccgc ccgacaccaa aaccgatggt
    10141 gaattcggca aaatgaaaat tgatgccggg aaatatgtgg ttgcccgttt tgatctggga
    10201 cccggagatt ttaccgaagc gtgggactgg gtctatggtt catggctccc aaccagcggc
    10261 tatcaacccg acgaccggcc ctgttttgaa ttgtatcccg aagaaccaaa agacggtcgc
    10321 ttcattgtgg atatatgcct gccggtgaaa ccgatgtaat ttttgcggaa aaattggcgg
    10381 ggcttagaaa aaaattttgt cattctgagc cctgctgctt ttatatcaaa aaaacttctg
    10441 aaatctataa cttctgaaat taaaaatctc aaatcttaaa atcttttatc acagagtctt
    10501 tgtgccatag tggctattgt aaacaacgcc tgatatttat cacatttctc tacaataata
    10561 gtcggaataa tagaaattgt gtatttttgc atttgaaaat gaaagacagt aaagcaagat
    10621 ccattgttaa aggcatcagt tggcgcgcag ttgcgagctt agatactttt gtgcttgggt
    10681 atatcatttt cggcaatgtt gcgcacgcct ctgccattgc gggtttcgaa gtgcttacaa
    10741 aaattttcct tttctttttt catgagcgtt tatggaatgt tgtgaaagcc ggccggcggc
    10801 ctgatggtag tgtagccccc tggcgctcgc tcgtgaaaag tatttcatac cgcttctggg
    10861 gtagtctcga caccactctg ctttcgtatc ttgtcaccgg aaacatcggc cattccttca
    10921 ttctgagcgg aacagaagtg gtaaccaaaa tcggattttt ctatctccat gagcgtgtat
    10981 gggcacatgt gaagtggggc cggatttatg aagaaacaga cacaaagttg tgcgaagttt
    11041 agaaacgttg cacaacttta atatctgaaa caccctttca catgatcatt caccagaccg
    11101 caggcctgca gatgcgcgta cacgatggtg gtgccaacaa atttgaatcc gcgctttttc
    11161 agatctttgc taatggcgtc ggagagcggt gttgaagaag ggatatcggc cagcaatttc
    11221 agttttgaat gaaccggctt attgtcaaca aaggcccaga gataggtaca gaaagttccg
    11281 aattcttttc tgacttcaat aaatgctttt gcattggtaa tggcagctgc aattttcaag
    11341 cggtttcgaa tgatgccggc atcctgcatc agccggttga aatccttttc ggtgaatttc
    11401 gccaccttct caaaatcgaa accagcaaag gctttgcgaa aattttcacg cttgcgcaga
    11461 atcgtcagcc aactcagtcc ggcctgaaaa gtttcgagaa tcatgaactc aaacattttg
    11521 taatcatcat gcaccggaat accccattct tcgtcgtgat atttgatgta gaggggatca
    11581 gcgatacacc acgcgcaacg ctgaatgttt tttgcagcca tgattttttt ctgtaaatat
    11641 aaaaaagctt acgctgtgcg aagtttaaac ttcacacagc ggagtctgtc acctttttaa
    11701 aattattttg tctgatatac cgaaacgtcc tatttttgac ccgccaaacc aaccttatga
    11761 tcaaaaaaat acttgtttgt tttgtcgtcg cgctgctggc gctgaacacc caggcacagg
    11821 atctgcccaa aggcatgacc gaagaagaaa aggccatgat gcccttttat cagtttccgc
    11881 aaatgcgcag cagcattacc actccgccaa catggacccc gcgcgccatg gccgaatggg
    11941 aagaaattga tgcgctgctg attacctgga catcctacac ttcggtgctg cgccagattg
    12001 tcgatgcagc acaggaagaa tgcaccgtac tgattgcctg cgatgactca aatgctgtaa
    12061 aaagttattt gaactcgggt ggagttccgc ttaccaacgt cgattatatc gaagtgcctt
    12121 acacatcggt ctggatccgc gattacggcg cacataccat ctatcagaat gttgtgggga
    12181 accattatct ggttgactgg gaatacaacc gcccgcgtcc cgaagatgac ctgatgcccg
    12241 aagagcacgc tgcctgggcc ggacttgatt tgtattccat gacagtatca ccctgggact
    12301 ttattgccac cggcggaaac ctgatggtgg acggctttgg caccgccatg tcgagcgaac
    12361 tgattgtaga tgaaaacccg tcgcattcgg ttgcggaaat cgacaccatc atgaaacgct
    12421 ttctcggaat cgaccagtac gtgctgtttc cgacattgcc ctacgacggc atacatcaca
    12481 ttgatatgca catgaaactg ctcgatgaag aaaccattct tgtaggtcag tatccaagcg
    12541 gcatttcaga cggaccccag attgaagcca acctgcaatg gctgctgacc aattatatga
    12601 gctgttttgg aacaccgtat aaagtgatac gaattccgat gctcccaagt gaagatggct
    12661 caacttggcc atcatctggg gcttactaca gaacatatac caacggagtg tttgttaata
    12721 aaacatatat atatccaagt tattatacaa aatacgatac tactgcaaat agaattctta
    12781 cggaatcttt accaggctat actattgtac ctattgactg cgatgcagga ggtaattctt
    12841 ctccaattgc agccagcgga gccattcact gcatcaccaa ctgcattggc agcaacgatc
    12901 cgctgctgat atcacatcaa ccgctgagca gtcctcaaac cggcagcaat tcatatcaga
    12961 ttgacgcata cattaaacat gaaagcggta ttgcctctgc caatttatat tacaaaactt
    13021 cgctggtcgg atcctatgtc agtgtgccta tgagcagcac cgggagcgac aactggacag
    13081 gatacatacc ggttcaggga ggtggaacaa ccgtatatta ttacattcag gcacaggcca
    13141 gcagcggcaa atcgcaggta agacccattg ttgcaccaga tggttattgg gaattcgaat
    13201 gtcagaacag cgttggcatc gaagaacccg ttgctgccat cggcatggaa accatttatc
    13261 cgaatcctgc tgcctacatc acctgtattc cggttgaatg cggaaacggc ttcacaggcc
    13321 gcctttcgat gatggatgct acaggccgca ccgttgctgt cattcacgaa ggcgaatttg
    13381 catctggtaa aaacaattat ttcttcaatg ccatggcatt cgaatcgggc ctgtatatta
    13441 ttcagctcga aaacgaagaa agcgtgcagc agcagaaagt gattgtaaga aatttctgat
    13501 tgctgaaaaa ttataattca aaaaagagga cccgtaaaaa cggatcctct tttaattttg
    13561 tcttcaaaac aggattattg cagcactacg cgcatttctt tcacctgatc tttaatggta
    13621 atacggattg aatacacacc cggcaattcg ttgctcatat caatactgcc aaaagtatca
    13681 ttcaaaacaa cttccttaac gatacgtcca tcaagactga aaacctgcac cagcatttca
    13741 tcctgaacat tatcagctct cagataaaat acatcgtccg aaggattcgg atatatgctg
    13801 agcgtaatgt cattttcatc aatacctgta caatcaataa aagtaatggt aatggtgtca
    13861 gaacccgtgc agccatactg atcggtaaca gtaacccata catcaaatgg tgtattgaga
    13921 ggagcacctg tagtatcaac atcaatggta gccgtggttt ctgaagttga ccaatcatat
    13981 gaggaaaatg ttccggggtc aagagttacg gtattctgaa cgcaaaattc gccatcggca
    14041 ccaaggtccg gagctggagt ctgaacatca atcagtacag aatcggggcc accatgaata
    14101 gtgaccggtt cgacaaacat ttcaacacgc ccaacccagt ttgagttttc tgtccaaagg
    14161 ttttgagcag gattgtttac aaaatcagtc tgagtaccca tccgatacaa tgtaacggaa
    14221 tctccaagta tagttgtcgg atatgttgga ttggcagtcc agctcattgt tgtaacagta
    14281 cctgaatatc gtgttcccaa tactccgata atatcacctt tgtggataaa agcattaacc
    14341 ggaacaattg cgtttcccgc aacatttgta tgccttccca atgatacaaa ttcattggtt
    14401 gtgacagaat atgatggagg aatattgttt aacctgacca cttcaatata ttgcgttcct
    14461 gcagcttcat ttggcactct tagcccggtt ataacgtgat cattcggagc cacgtaataa
    14521 aaccctctag ccattaaaga actgtaggtt gaagtgctgg tttgagtcga aacatcaact
    14581 gtgtcaacat ttcttacaga atagtacaat gtaccattcc aaattctgga actagaacaa
    14641 aaaaaggcat ttgagccgcc ataatgtcct gtcagaacca tgttactgtc ggtataaaca
    14701 tcattggttc cagcaacaat acctgtatat ctcatgtcac tgacagaaca cacatacaaa
    14761 ccataaaggt ttccggcccg aagcaaaatg cggtttgaaa gcggaacacg ggtaggaata
    14821 tcctggcctg aagcaacaac aacaacactg tcaaccaaag accagccagc attgctggta
    14881 ttatttccaa ctactgttcc gttcctaaca tataatttta cccaaccatc actaataata
    14941 ttcacatcga gactatcaat cacaatgtcg ttttttgcga aaacgtcaaa catgttaccg
    15001 ttatactgat tattactggc aaagattgaa gttatagatt tatcttcctt atgttcttcg
    15061 gccactttgt aataaatgcg gccattccat acacgcggag aaaaaatcac actaaaaaat
    15121 gcgccaccat taccaaaatg gcaggtcata tcatcattga caatagtttc atttgtaccg
    15181 ttacccgttg tataatatat accaacgctt gtgcagatat aaaggcccat tgtctgacct
    15241 tcagcaatag tcaatgattt tgtcaggtca agacgagcat gataatcgac tccgttctga
    15301 atcaggttaa ctgagtccag caaaatccat cctacatttg aagattcaaa accgacgtat
    15361 gatccagatc tgtaatacac ttttgccgat gcagcagttg ttgttcccag attgaggtcg
    15421 aaaccatcaa tcacaatgtc ttttgctgca acaacatcaa acatatttcc accccaggcc
    15481 aaatcatagt tattattgcc aatccagttg ctttcaataa acggataacg atattcaacc
    15541 atttcatcag cagcaaaagg tccaacctgg aaccatgnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnngga taacgatatt caaccatttc atcagcagca aaaggtccaa
    15721 cctggaacca tgtactgtct ttgtaaaaac ccgctgaatc aagtacaaca gccggagata
    15781 cactaatctc atccactacg ttcgcacaga ttttagtgtc ttcaaatgca ggataatcaa
    15841 agtttgaaac tcctgcataa atgccatccc accatgcttc ggcgccagca gtctgattgt
    15901 acacatcgag tgtagagata gaagaaacag atccgcggaa aggcagtcca ttgcctttta
    15961 aaacgccatt gagatacaca tcatacgtca tgtctgtgaa attcatattt ttcacttcca
    16021 cgcgcagcca ttcattactc actaccggtc ccagattggt tgttccggtg ctgccagtaa
    16081 caacaaaatt accaccggta atttcaccat aaaaagcagc attgattgca gagtggccgt
    16141 taccggcagc aacaacacca ccatctccgc caacaacttt tacataataa aacaatacgc
    16201 tcagattttg actggcagca aaatcgagac ttaaaccttc accaacaccg tttccggtga
    16261 tatgcaagga tttttcgccg atgattgcat caactgtgct tgcttcaaca gttccccccg
    16321 ttgaaagatt gttccatttt gccagcccat tttcaaaact ttccgtaaaa ggaatcgttt
    16381 ggccggcagc aacaatcaca catgcaacca tcatcaagac agataaaaat gttctcataa
    16441 atttgatttt ttgcaaatat aaaacaatta taccaatatg actattttgt cgttcaacaa
    16501 atatgttttt aacacattat gtaatcatta atacactttg tataagaaaa ttacatctca
    16561 acaacctttt aattttttgc aaatctattc ttccaggatg cattgtattc cggaatcaca
    16621 agacaggcga gattatgcaa aagcataaaa tcaatacctg aataatttcc gagcgcccag
    16681 ctttcgccgg ttttgccatt ctgggcagct gtagttgccg accacttgaa ttccgccgac
    16741 catcctggtg ttttgggata gctgctattg ccatactgcc acgttccgtt tggcggagcc
    16801 tgctgcaact gtgtcaatag tctgttaaaa tcataaagcg taccatcaac ttcaatgtta
    16861 ttcaacgaag catgcaacaa caaataaaag gtgtcccact cataatcatc cgttatcaga
    16921 taaattattt tcggaacatt gttaccgact ctcgaaacac cagatacaga cattgcttcg
    16981 gccatcagaa aacgtaaaat cctgtattcg gaataaccga taccactcaa gcaggccagc
    17041 tctgttccct catatgcatc gcggcaccag ggtgccctga caatagccgg atagtgtttt
    17101 ttcagatatt cctttccgac caatttataa gccagtgttt tcagggcaaa agcataagcg
    17161 cgtccatcgg cgccgttttc gtttcccata cgtgtgccat cgggaaacag cggccgccac
    17221 cagagcgttt tgcgactgcc acacatgaag ttgatcattc gcaaaatttg attccgtgct
    17281 gattttcgta ttgcctcgtc atcaacaaat ttaattgtta gcgccagtcc tacaaatgaa
    17341 cccaacactt catcctgtga tgtaaatttc tgatttttat aataatgcaa gtattgtttg
    17401 tttcgacggt taaatgcagc ctcattcggg tgacctgatt ctcttttggt aaaaaaatat
    17461 ccatcaggcg attcatacat tttcaatgct tcaacagaat tattcataat ca
//
LOCUS       DAOPKE010000059        17228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3498_length_17228_cov_12.526058, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000059 DAOPKE010000000
VERSION     DAOPKE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_17228_cov_12.526058"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..481)
                     /locus_tag="PKY63_08520"
     CDS             complement(14..481)
                     /locus_tag="PKY63_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00695.1"
                     /translation="MKVFFLSFFLIVFFVSCSSDQSNNQTYYYGFVNNTWNIDQSVSF
                     SFDIADSSVHYDVYGKMRMGKNFTFSSLDMSLVLLTPSGSSRFKKIHLPMRNDDGERT
                     GNLINDYYEIPFPVYDSIRFAEPGKWTMNFNHNMPVDIYEGMIGMEIFIEEKK"
     gene            complement(478..1686)
                     /locus_tag="PKY63_08525"
     CDS             complement(478..1686)
                     /locus_tag="PKY63_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPF00696.1"
                     /translation="MDNTEIISSLAAKIYPRLVAVRRQLHKYPELSGQEKNTAALICS
                     VLDEWNIPYKAGIGGYGVVALIEGKSPGACVALRADMDALSIQEQNLCDYKSMVDGVM
                     HACGHDAHVASLLGSAFILDYLKNTFDGTFKLVFQPSEETFPGGAIKMIQDGVLDYPK
                     VNAMIGQHVLPGLPTGSIGLKSGPYMASTDEIYITVTGRGGHGATPELNIDPVVIASQ
                     IIVALQQITSRAANPSIPTVLSFGRMIADGRTNVIPDSVTMEGTIRTFDENWRAEAHQ
                     KIVSIAKGIAEGFGAQCTVDIHHGYPFLINDEKTTRIARLAAVEFLGADKVSDLPLRM
                     TAEDFAYYAQNVPAVFYRLGITNPLTNAPSANLHTSGFDIDENSLITGSSCMAFIALK
                     TMSDLNESEI"
     gene            complement(1689..4856)
                     /locus_tag="PKY63_08530"
     CDS             complement(1689..4856)
                     /locus_tag="PKY63_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00697.1"
                     /translation="MSFGKNRVQYNEERIWSQFRFKDYDFVFYQDGRKITINAARYAS
                     SALKEISEKLSYKSESKIHFIVFNSLSEMKSSNIGLDNEVLYNTGGVTHVVDNKVFLY
                     FNGNYLNLEEQIRGGIARVILNQMMYGEQISANMKNSTLMSLPEWYLSGLQSYLASDW
                     NTELDEKMRDAFVSGKFSKFNHLEGADATLAGHSVWRFIAGKYGGNMIPNIIYMAKVS
                     RNVENGFLFVLGISFKNLIREWYAHNQAVYLNDVNGRDLAPDHNVPVKIKRKNHYYQA
                     KVSPDGRYLTYVMDKVNKKKLFIVDRQSEKKKKLFRLGTKMNDIPDMTYPLTAWHPTS
                     GLFAWEVENKGRRKLYLYNVEDKTREEFFIDNINKVLDMAYSPDGSLLVMSAVINGYS
                     DIFLFYTGSKSFVRITNDVYDDLQPRFIDNGRMIAFASNRISDTLVIEQETYLVDYSI
                     PVNKQQSFDIFAYDVVSKPQILRRVTNTPAVSESSPMPWNGKYFSFISDENGIRNQLI
                     GHFDSAIAYVDTSIHYRYFTSVSPMTNYASSIREQESYLRSDFVSLVFTVGKSSRIVV
                     AEKNITGDFASGSEPLNTAWADERNLIFSMYQTKPDTLGQNAKNAQKQGFVKSQTDKG
                     NVNVYNYRFTTNTNKNPKPNVVAKTDSVEKFSLPHQQNYDVEYGIDKLVSQLDFSFLN
                     TTYQPYNRSVVTFGNAGNNALFQISASDLLEDKRITGVVRSSFSLDNNEYFISYEDLF
                     KRLDRQIVFHRMTYDAANETSYVKHHLHDVNYIFKWPFSPVLALKGTLILKYDNQVYK
                     SIDDFTLAQPDVNTFWGGTRIELVFDNSRPVMTNIMYGMRYKVFAEYYQGINKSELNL
                     ITAGLDFRHYSKIHRTFIWANRLAFGTSFGSTRLLYYLGGTDNTFLPSFNYSQPVDTS
                     MNFAFQTLGTNLRGFPQNIRNGNTFAVINSELRFPVFRYLLNRPIKSQFINNFQIVGF
                     GDIGSAWLGLNPYSEQNIMVPETYYQKPITVVVKSPRNPLVGGIGAGVRSVIFGYFVR
                     FDVAWGIEELQVYSPRYVLSFSLDF"
     gene            5082..>6426
                     /locus_tag="PKY63_08535"
     CDS             5082..>6426
                     /locus_tag="PKY63_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPF00698.1"
                     /translation="MTRLAFTIPLFLMLITNSIAQNGFELSGQISNFQRPATIYLLSV
                     EGSEWDVADSMKSIKGEVLFSADTIPVTGEYYVFWEEKYFINLVINNERRIEFTADNL
                     EENLAVHIQISEENKAFYQLRAIENSIDSLSAIGDEYYEKGMHAQLQKIRNQLQLKIA
                     ELEKKIQQTDAERPGLFSVKLYKSSIPPDFNSFALSNPDHGFKTEYDFLKRHYFDNID
                     KYDSCLVNTRVIYDACSFYLRNFTDEKNTAGYIKTVDFIISSFSWNNKQFDYVLNLLL
                     NTFESAGFDDVYLHLFDTYMHGTSCDGGIPGEAERKALSIKNMKKGTFAPELTGFDKN
                     GNSVSLSDFKGKTVIVMFWESSCEHCRNAIPHVTALIKDKPDIVLLSYSIDTDENKWI
                     GGCLEEGLPEPSISDLKGYDGANAIRWNVWGTPSFFVIDTEGKIAAKPLTLSALED"
     assembly_gap    6427..6526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6630..8687)
                     /locus_tag="PKY63_08540"
     CDS             complement(6630..8687)
                     /locus_tag="PKY63_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00699.1"
                     /translation="MGRFVSFCLLLALFSFFSHAQENSISSRNGQVLPVKGTIRILLV
                     FVEVEYPAGVEKNATDTNEFWKPGAYPVWASDLFDVFPSSDPKGLVSRYYHECSFGQY
                     NVVADILLNPDNPEIPFQYKSKGDINVASILNEAWNKGFMTKAALPADSFDLWVKSAP
                     GKQKLIRTPDSPLSFDHVMVIVRNCTYPGHLAGYASSGNVSRNVLFKTDSYSVFSSRS
                     ISPFNILLHEYNHLLFGGNNLHCCGGNHSSSGPQLFLSFQGGWGMMGAANKSLLSCNA
                     WDRYKLGWKRPEKEYYISALNFSAKEVNTDFDFRNEYIVDTLIILRDFITSGDALRIR
                     LPGVPDDEYQQWLWIENHQTKSFNGSLFDVFQYEESGCTDHAVPGIYAYLQVSHDDLY
                     GKNVFGGYADFIRSLPASGMYDFVWGDTLVRNDWCLNNGLYYPFERKPALMNPLSGNA
                     VTEIVAFDNNFDGHLEEKEKRDPAIERVSGGYVNNLPYLGESEMAFVFHGNNKIAVGT
                     NPSSANALTLLNDNRSINKGMPPDNRVIYLNSVSVEILRENWPSPGDVLLRVRNGDNV
                     VSQNVRWSAPKIVLPDLPSDNQFDLIIDKRRRVIIDAGLTPTRIDSVIIVKGKTVITD
                     YTHFQMMPGTRLLLRKGARLDIRNKSVFHVSPDAVIVLEKGARIIVGRDSKLINEGKI
                     IKR"
     gene            8761..11217
                     /gene="pheT"
                     /locus_tag="PKY63_08545"
     CDS             8761..11217
                     /gene="pheT"
                     /locus_tag="PKY63_08545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPF00700.1"
                     /translation="MKISYNWLKQYLSTDLPAEIISKILTDNGLEVEGLEEHEKIKGG
                     LEGIVVGHVLECAHVEGTEHLSLTKVDVGTEQLQIVCGAPNVAVGQKVLVALPGATLY
                     KSDGTSLTIKKAKMRGIESNGMICAEDELGLGSSHAGIMVLDSIAETGLPAAKFFNIS
                     SDYVFEIGLTPNRSDAMCHTGVARDLLAALHNRCGEFSTELQMPSVNAFKADNNDLTT
                     PVEVKSPEACIRYSGLTLTNLNVTESPDWLKIKLESVGLRPINVVVDVTQYVMLELGQ
                     PLHAFDYDKISGGKVIVQCLPDDTMFTTLDGVERKLTSRDLMICDAEKGMCIAGVFGG
                     EHSGVSMETKRVFLESACFNPVSVRKTAKHHGLHTDASFRFERGTDAEITIYAIKRAA
                     LLLKELTGCNISSEISDYYPVKVEKPAVVLNIASMNAFAGSPIPTDTVAGILKDLDFV
                     ITKQTPEELTIEVPLNRTDVTRPVDVYEEIFRIYGYNNIPMPEKSNTIYPKNTGISLN
                     ELIQKVSDFLAAKGFSEIMCNSLNSMQWYEKASGYPLENIVNIQNPLSRELNVMRYSM
                     LPGMLQSAAYNINRNTANLKFFEPGKTYVKNTTDKEKPVTARFSETNMLSLLMTGSER
                     PENWKYTAETSDFFSIKSALEDLFDFLGIINSVVARTEVNHQFTFGMTYILNEKTLAA
                     FGLVKNELRTLSDVDNEIWYAELNLDLLLKKIRKTNMRYQDIPKYPAVRRDLALLIDS
                     NSTFESIQKAALGAEKKLLKKVDLFDVYVDKKLGDNKKSYAISFMFRHDEKTLTDAEV
                     DKAMDRITTEVLKVTGGVIR"
     gene            complement(11267..11623)
                     /gene="folB"
                     /locus_tag="PKY63_08550"
     CDS             complement(11267..11623)
                     /gene="folB"
                     /locus_tag="PKY63_08550"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPF00701.1"
                     /translation="MEIISIEGMEFRAPVGCFDEEKIVHPGFSVDVYISFDASQAMLS
                     DKLETTINYQAVYLRIKEIMEVHHNIIEHVASHILDTVLSEFLLAQHVKCKIHKTFPA
                     LGGRIAAVAFEAERGR"
     gene            11676..13355
                     /locus_tag="PKY63_08555"
     CDS             11676..13355
                     /locus_tag="PKY63_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPF00702.1"
                     /translation="MADEKINLPEEGKVQKNFIEEIIDSHYESGRFDHVLTRFPPEPN
                     GYLHIGHAKSICLNFGLARKYNGKCNLRFDDTNPTKEDVEYVDSIKEDVKWLGFEWAN
                     EFYASDYFQQLYDWAEKLIADGKAYVCECSQETISANRTTPTRPGIACEHRNRTPEEN
                     MQLFRRMRDGEFPDGSMVLRAKIDLNSPNMHMRDAIMYRILHAEHHRTGNKWNIYPMY
                     DYAHGQSDSIEGITHSVCTLEFEVHRPLYDWFVANLGIHAPQQIEFARLNLSYTVMSK
                     RKLLQLVNEKHVSGWDDPRMPTISGMRRRGFTAESIRLFAEKVGVAKRENVIELGLLE
                     FCVREDLNKRAERRMAVLNPVKLIITNFDDTNIDLLTAINNPEDETAGTREIPFGREL
                     YIERDDFMEEPVKGYHRLYPGNEVRLRYAYFVKCTGCKKDENGNVIEVYGEIDPASRG
                     GNSPDGRKVKGTIHWVPAKQSVACETRLYDRLFTVPAPDDAEEGKTFLDYLNPNSMKT
                     VTAYCEPALTAAKPGQYFQFERLGYFVPDKESPAGKPVFNRTVTLKDSWGK"
     assembly_gap    13366..13465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13906..15585)
                     /locus_tag="PKY63_08560"
     CDS             complement(13906..15585)
                     /locus_tag="PKY63_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00703.1"
                     /translation="MNKIFAVSIILLSLLPQLIHCQTPQWHWAKGFGGDYVDKANALA
                     TDADGNVYMAGGFYSSSITFGNTTLYNTGVSDIFIVKFDSSGQVIWANSFQGSAGSNN
                     KATALTVDSHGNVIITGVFESPSLYYNGYAVNCTCLRDFFVAKISPSSGLIWMKAAVG
                     PAFDGSSSICTDQNDNIFIAGGFDSYVLSMDDVVLDKKGNGNPCTNAFIFKLNENGEA
                     VWGTNFGEDSDDGIVIRAIKVKPDGNIVFCGNFATPRMGIGPDTIFNLDTIPPSISLD
                     AFVIEMTPDFEYSDVKQIAGTDHDNIYDMDIDNEGNIVFTGCFNSNPLIIGSDTLTYI
                     PPNTDAFTAMISSSGDFLWARSIGDDPIVADYNNCVFDSKGKIFITGQYMYDTLFIAN
                     DTLVGHSFPDFLLLKYNSEGETEYCRGAGDEGFDEAFALTIDKENNIYIAGSYTSSVL
                     VIGNDSLINIGAENCFLAKFSEYRRHDSLPDISFAVYPNPSDGFITVNPGSDFSQGYN
                     LEVTNILGQRVFYMQADDRQLQELFLPFASGVYCVTLYTQLTRKSAKIIIE"
     gene            16032..16844
                     /locus_tag="PKY63_08565"
     CDS             16032..16844
                     /locus_tag="PKY63_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00704.1"
                     /translation="MVKTIPAILSTILLLSCNFSNPENNANHYAFRDIKTCFFYNYLL
                     EKNPANLPYFENQIIQELSKCINQKILFEELIQDSTCWLVLECIDFNTSPAGIRQPGF
                     EIFLDSCGSMSHNCKSTCPEELADKYISYLDWRSQNFRRIRLTDIADKEMIISPEMGR
                     VFKSRGFLEMGIETNPENSAQIKQIRNIRTFIREVASKYYDFRDETAYKYFSKSLKDL
                     SKTEWIKTVKLSEFYSEFHFFTSDPDSLNLFLIPCDHKKLIQIPNREHGIID"
     gene            complement(16834..>17228)
                     /locus_tag="PKY63_08570"
     CDS             complement(16834..>17228)
                     /locus_tag="PKY63_08570"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPF00705.1"
                     /translation="ILVGGNLSVLYSMLGSASFPDMHGKILFLEDLDEYLYHIDRMML
                     GLKRAGVFDNLAGVVIGTMSEMHDNETPFGKDAETIIRDQLCEYSFPLLFGFPAGHTE
                     LNRAFFVGAETEISVKSGGADFLQEISQ"
BASE COUNT         5513 a         3549 c         3403 g         4563 t
ORIGIN      
        1 gtgagcggtt tttttatttt ttttcttcaa taaaaatctc cataccaatc attccctcat
       61 aaatatcgac aggcatatta tgattgaaat tcattgtcca tttgcccggt tctgcgaagc
      121 ggattgaatc atagacaggg aagggaattt cataatagtc gttgatcaga tttcccgttc
      181 tttcgccatc atcattacgc atcgggagat gaattttttt aaagcgtgaa gagccggatg
      241 gagttagcaa aaccagactc atgtcaaggg atgaaaaagt aaaatttttc cccattcgca
      301 ttttcccata aacatcataa tgaactgatg aatcagcaat atcaaatgaa aaagagactg
      361 attgatcaat attccatgta ttgttgacaa agccgtaata gtaagtctga ttatttgatt
      421 gatcagaaga acaagaaaca aaaaaaacaa tcagaaaaaa cgacaaaaaa aagactttca
      481 tatttcactt tcattcaaat ctgacattgt ttttaaggca ataaaagcca tacaagaaga
      541 accagtaatc aatgaatttt catcgatatc aaaaccagaa gtatgaagat ttgccgatgg
      601 agcatttgtg agcgggttgg taataccaag acgataaaaa acagctggaa cattttgggc
      661 ataataggca aaatcctctg cggtcatacg caaaggaaga tctgaaacct tatccgcgcc
      721 aagaaattca acagcagcca aacgggcaat acgagtcgtt ttttcatcat tgatgagaaa
      781 tggataacca tgatggatat caaccgtaca ttgagcgcca aaaccttcag caattccttt
      841 tgcaatggaa acaattttct gatgtgcttc ggcccgccaa ttttcatcga aagttctgat
      901 tgttccttcc attgtcacag aatcgggaat tacattggtg cggccatcgg caatcattct
      961 tccgaaagat aaaaccgttg gaattgaagg attggcagca cggcttgtta tctgctgcaa
     1021 ggcaacaatg atctgcgaag caataacaac cggatcaata ttcagttcag gcgtggcgcc
     1081 atgcccgccg cggccagtga cggtgatata aatttcgtca gtcgaagcca tataaggtcc
     1141 gcttttcaga ccgattgaac ccgttggcaa acccggtaaa acatgttgac caatcatagc
     1201 atttaccttt gggtagtcga gtacaccatc ctgaatcatt tttatggccc cacccggaaa
     1261 agtttcttca gatggttgaa aaaccagttt aaacgtacca tcaaaagtat tcttcaaata
     1321 atcaagaata aaagcagatc ccaaaagcga ggccacatgg gcatcgtggc cacatgcatg
     1381 cataactcca tcaaccatgc ttttataatc acataaattt tgttcctgaa tgctgagagc
     1441 atccatatcg gcgcgcaaag cgacacaagc tcccggcgat tttccttcaa taagtgcaac
     1501 gacaccatat ccgccaatac cggccttata aggtatattc cattcatcaa gaacagaaca
     1561 aatcaaggcc gcagtatttt tttcctgacc agacagttcg ggatatttat gcaactgacg
     1621 acgtacagca acaagtcggg gatagatttt agcagcaagc gatgaaataa tttcagtatt
     1681 atccataatt aaaaatcaag actaaaagac aggacatatc gagggctgta aacctgcagc
     1741 tcttcaattc cccatgcaac atcgaatcgg acaaaatagc caaatatcac agatcggaca
     1801 ccagcaccta taccaccaac caaaggattt cggggagatt ttacaacaac agtgattggt
     1861 ttttgataat atgtttctgg caccatgata ttttgttctg aatatggatt caacccaagc
     1921 caagcagaac cgatatcgcc aaagccaaca atctgaaaat tgttaataaa ctgcgacttg
     1981 attggacggt taagcagata gcggaagacc ggaaaacgca attcggaatt aataactgca
     2041 aaagtatttc cattgcggat attctgcggg aatccacgaa gatttgtgcc cagtgtctga
     2101 aaagcaaaat tcatcgaagt atctaccggc tgactataat taaatgaagg aagaaaagtg
     2161 ttatccgttc cgccaagata ataaagcaat cgggttgagc caaacgaagt accaaaagcc
     2221 agacgatttg cccagataaa ggttctatga atttttgaat aatgccggaa atcaagacct
     2281 gcggtaataa gattaagttc agatttattt atcccttgat aatattcagc aaaaacctta
     2341 taacgcatac catacataat gtttgtcatt acgggtcgcg agttatcaaa aacaagttca
     2401 atccttgttc caccccaaaa tgtattaaca tcaggttgag ccagtgtaaa atcatcaata
     2461 cttttataaa cttgattatc gtattttaag atcaaggtgc ccttcagtgc aagaacagga
     2521 gaaaagggcc atttaaagat ataattcaca tcgtgcagat gatgtttgac gtatgaagtt
     2581 tcatttgccg catcataagt catccgatga aaaacaatct gccggtcaag acgtttaaac
     2641 aaatcctcgt aactaataaa atactcattg ttatcgagcg aaaaagagga gcgaacaaca
     2701 cccgtaattc ttttatcttc gagtaaatca gaagcgctta tctgaaaaag agcattattg
     2761 ccagcattcc caaaagtaac aacagacctg ttgtatggct gataagttgt attgagaaaa
     2821 gaaaaatcga gttgagaaac aagtttgtca atgccatatt ccacatcata gttttgctga
     2881 tgcggcaaag aaaatttttc aaccgaatca gttttagcca ctacgttagg ttttggattt
     2941 ttattggtat ttgtagtaaa acgataatta tatacgttaa catttccctt atcagtctga
     3001 gacttaacaa aaccttgttt ctgagcattt ttggcatttt gacccagtgt atccggcttt
     3061 gtctgataca ttgaaaaaat cagatttcgc tcatctgccc acgcagtatt taatggttca
     3121 gacccggaag cgaaatcacc tgtaatattt ttttcagcaa caacaatgcg tgaagacttt
     3181 ccaaccgtaa aaaccagaga aacaaaatca gagcgaagat atgactcctg ttctctgatt
     3241 gaagatgcat aattggtcat cggcgaaact gatgtaaaat accggtaatg tattgaagta
     3301 tcaacatatg caatagcact gtcgaaatga ccaattaatt gatttctgat tccattttca
     3361 tcagagatga aagaaaaata ttttccattc cagggcatgg gagaagactc tgaaacagcc
     3421 ggagtatttg ttacgcggcg cagaatttgc ggttttgaaa cgacatcata ggcaaaaata
     3481 tcaaacgatt gctgcttatt gacaggaata gaataatcaa ccaaatacgt ttcctgttca
     3541 attacaagtg tatcacttat gcgatttgaa gcaaaagcaa tcatgcggcc attatcgatg
     3601 aagcgaggct gtaaatcatc ataaacatca ttagtgatac ggacaaaaga tttactaccc
     3661 gtgtaaaaaa gaaaaatgtc gctgtagcca ttaatcacag cagacatcac cagtaaggat
     3721 ccatcgggag aataggccat atcaagaacc ttattgatat tatcgataaa aaactcctca
     3781 cgggttttat cttcgacatt atataaatac aacttacgtc gtcctttatt ttcgacttcc
     3841 caggcaaaaa ggcccgaagt aggatgccat gctgttagcg gataagtcat atcagggatg
     3901 tcgttcattt ttgttccaag ccgaaacaat ttctttttct tttcggactg acgatcgaca
     3961 ataaaaagtt ttttcttgtt caccttatcc attacatagg tcagataacg tccgtcgggg
     4021 cttacttttg cctgataata atgatttttt cttttaattt taacaggcac attgtggtcc
     4081 ggagccagat cgcggccatt cacatcattc aggtaaaccg cctgattatg agcataccat
     4141 tcacggatga ggtttttaaa agaaatgcca agaacaaaca gaaacccatt ttccacattt
     4201 cgagaaacct ttgccatata aatgatgttt gggatcatat tccctccata tttgcctgca
     4261 ataaagcgcc agacagaatg accggcaaga gtggcatcgg caccttcgag gtgattgaat
     4321 ttactgaatt ttcctgacac aaaggcgtcg cgcatttttt cgtccagttc agtattccaa
     4381 tccgacgcca gataggattg aagaccgctg agataccatt ccggcaacga catcagcgtt
     4441 gagtttttca tattggcaga tatctgttcg ccatacatca tctgattcag aataactctg
     4501 gcaataccac ctctgatttg ttcctcaagg ttcagataat tgccattaaa atacagaaaa
     4561 accttattgt caacaacatg agttactccc ccggtgttat acagcacttc gttatcgaga
     4621 ccaatattgg aagatttcat ttcactgagg gagttaaaaa cgatgaaatg tatcttgctt
     4681 tcagatttat aactcagttt ttctgatatt tccttcaaag ccgaagaagc atatcgggcg
     4741 gcattaatgg taattttacg accatcctga taaaagacaa agtcataatc tttaaatcga
     4801 aattggctcc agatacgctc ttcgttatac tgaaccctgt ttttaccaaa tgacatatgg
     4861 ctcccattat aaaactgagc agaaaccttc tgaaaaggca tcaaaagggt aaaaaggaca
     4921 attatgacga aagttttgcg cataaccatt ctaccgtttt aaacaacggg attgtaaaat
     4981 tacgaaaatt taccggtttt attgcaatac agatgtcaat aaaaaataac ttttataaaa
     5041 ggcccggaat ttgattaatt ttgacctcga aaaaacagtg tatgacacgt cttgcattca
     5101 ccattccgtt atttttaatg ctcatcacca atagcattgc gcaaaacggt tttgagctaa
     5161 gcggtcaaat cagcaacttt caaaggccgg caaccatcta tcttttgtct gttgaaggat
     5221 ccgaatggga tgtggcagac agcatgaaat caataaaagg cgaggttttg ttttcggccg
     5281 acacaattcc ggttactggg gaatattatg ttttttggga agaaaaatat ttcatcaatc
     5341 tagtaattaa caatgagcgc agaatagaat tcaccgctga caatcttgag gaaaatcttg
     5401 ctgtacacat acaaatttcg gaagaaaaca aagcgtttta ccaactacgc gccatagaaa
     5461 attcgattga tagcttatcc gccattggcg atgaatacta tgaaaagggg atgcatgcac
     5521 aacttcagaa aatcagaaat caattacaac taaaaatagc tgaacttgag aaaaaaatac
     5581 aacaaactga tgctgaaagg cccggactat tctcagtcaa attatacaaa agcagcattc
     5641 cacccgattt taactctttt gcactgtcaa atccagatca tggatttaaa actgagtatg
     5701 actttttaaa acgacactac ttcgacaaca ttgacaaata cgacagttgt cttgtaaata
     5761 cgcgtgtaat atatgatgca tgcagttttt acctgagaaa ttttacagac gaaaaaaaca
     5821 cggccggtta tatcaaaact gttgatttca ttatcagttc tttttcatgg aacaacaaac
     5881 aatttgatta tgtattgaat cttttgctca acacatttga atcggcggga tttgacgatg
     5941 tttatctgca tttgtttgac acatacatgc acggaacttc ttgtgatggc ggcattcctg
     6001 gagaagccga acgaaaagca ctttccatca aaaacatgaa aaaaggcact tttgctccgg
     6061 aactgacagg gtttgacaaa aatggcaact cggtatcgct gagcgacttt aaaggaaaga
     6121 ccgtcattgt gatgttctgg gaatcatcgt gtgagcattg ccgcaacgcc attccgcatg
     6181 ttacagcact gataaaagat aaaccagaca ttgtgctgct ttcatattct attgatacag
     6241 acgaaaacaa atggataggc ggatgcctcg aagaagggct cccagagcca tctataagcg
     6301 acctcaaagg atatgacgga gccaatgcca tacgatggaa tgtttgggga acaccaagct
     6361 tttttgtcat tgacactgaa gggaaaattg cagcaaaacc gcttacattg tctgctttgg
     6421 aagatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttaa agggttaaag
     6541 ggttaaatga ttaaagggtc aaatataaat gaataaaaca ggcgaaatca gtaaaggatt
     6601 aaacccggaa taatggggtt ttacaaacat cagcgtttaa taattttgcc ttcattgatg
     6661 agctttgaat ctctaccaac aataattctg gcaccttttt ccagaacaat aacagcatcg
     6721 ggagaaacgt gaaaaaccga tttgttacgg atatcaagtc gggcgccttt gcgcagcagc
     6781 aaccgtgtac ccggcatcat ttggaaatgg gtataatcgg taattaccgt tttgcctttc
     6841 acgataatca cagaatcgat gcgggttgga gtcaatccag catcaataat aactctacgc
     6901 cttttatcaa taataaggtc aaactgatta tcggaaggca aatcgggtaa gacaattttc
     6961 ggagcactcc atcttacatt ttgcgaaacg acattatctc cattacgaac acgcagcagc
     7021 acatcgccag gagaaggcca attctctcga agaatttcaa cacttaccga attcagataa
     7081 atgacacgat tgtcaggcgg catgccctta ttgatggatc tattatcatt gagcaaagtg
     7141 agagcatttg cagatgaagg attggttccc actgcaattt tattgttacc gtgaaaaaca
     7201 aaggccattt ccgattcgcc aagatatgga agattattta cataaccgcc agagacacgt
     7261 tctatagccg gatcacgttt ctctttttct tccagatgtc catcaaaatt attatcaaaa
     7321 gccactattt cagttacggc attaccgctc aacgggttca tcagtgcggg ttttcgttca
     7381 aaaggataat acaatccatt attcagacac caatcatttc ttacaagggt gtctccccac
     7441 acaaaatcat acattccgct tgcaggcaat gaacgaataa aatcggcata accgccgaat
     7501 acatttttac catacaaatc atcatgtgaa acctgtaaat aagcataaat gccaggaaca
     7561 gcatgatcgg tacagccaga ctcttcatat tgaaacacat cgaataaact tccattaaaa
     7621 gattttgtct gatgattttc aatccacaac cattgctgat attcatcgtc agggactccc
     7681 ggaagtctaa tccggagcgc atctccagat gttataaaat cgcggagaat aattaatgta
     7741 tcaacaatgt actcatttcg gaaatcaaaa tctgtgttta cctctttagc tgaaaaattc
     7801 aatgcagaaa tataatactc tttctcaggc cgcttccagc caagcttata acgatcccat
     7861 gcattgcaag acaacagtga tttattggct gctcccatca tgccccatcc tccctgaaaa
     7921 gacaaaaaca attgcgggcc gctgcttgaa tgatttcctc cgcaacaatg aaggttattg
     7981 ccgccaaaga gcaaatgatt atattcatgc aaaagaatat tgaaaggact gatactgcgc
     8041 gaagagaata cgctatagga atccgtttta aacaagacat ttcttgatac atttcctgaa
     8101 gaagcataac cggcaagatg accaggataa gtgcaatttc ggacaatcac cattacatga
     8161 tcaaaagaaa gtggcgaatc tggtgtccgg attaactttt gcttaccagg agctgattta
     8221 acccaaagat caaaactatc ggcgggcaaa gccgctttgg tcatgaagcc cttgttccat
     8281 gcttcattca gaatagaagc aacattaata tcacctttgc ttttatactg aaacgggatc
     8341 tccggattat caggattcag cagaatatcg gcaacaacat tgtactgacc aaaggagcac
     8401 tcatgataat atcggctaac caatcctttt ggatcagacg acggaaagac atcaaacaaa
     8461 tcactggccc aaacaggata tgctccgggt ttccaaaatt catttgtatc cgttgcattt
     8521 ttttcaacac cagccggata ttcaacttca acaaacacca ataatatcct gatggttccc
     8581 ttcaccggta aaacctgacc attacgggaa ctgatactgt tttcctgagc atgagaaaaa
     8641 aaagaaaaca gcgccagcaa caaacaaaat gaaacgaaac gacccataaa aaattaatta
     8701 tgtaaatata caaattctga aaaaagggaa ttcagtatct ttgctgaaaa tattctcagg
     8761 atgaaaatct catacaactg gctgaagcaa tatctaagca ccgatctgcc agcagaaatc
     8821 atttcaaaaa ttctcaccga caacggattg gaagtagaag gactggaaga acatgaaaaa
     8881 atcaagggcg gattagaggg aattgtcgtt ggtcatgtgc ttgaatgcgc gcatgtagaa
     8941 ggcaccgagc atctttctct cacgaaagtt gatgtaggga ctgaacaatt gcagattgta
     9001 tgcggggcac cgaatgttgc tgtcggacaa aaagtgttgg ttgccttacc aggagccaca
     9061 ttgtacaaat cggacgggac atccctgacc ataaaaaaag ccaaaatgcg cggcattgaa
     9121 agcaacggaa tgatctgcgc cgaagatgaa ctcggtctgg gctcatctca tgccggaatt
     9181 atggttcttg attctattgc cgaaacagga ttgcctgctg caaaattctt taatatctct
     9241 tctgattatg tttttgaaat cggcctgacc cccaaccgaa gcgacgccat gtgtcacaca
     9301 ggagtggccc gcgatctgct ggccgcactg cataaccgat gcggcgaatt ttctacagaa
     9361 ttgcaaatgc cttcggttaa tgcttttaaa gcagacaaca atgatctgac aacacctgtt
     9421 gaagtaaaaa gtcccgaagc atgtatccga tacagcggat taacactaac caacctaaat
     9481 gttaccgaaa gtcccgattg gctgaaaata aaactggaat ctgttggact tcgtcccatc
     9541 aatgtggttg tggatgttac gcaatatgtc atgctggaac tcggacaacc gctccacgcc
     9601 tttgattacg ataaaatcag tggtggaaaa gtcattgttc aatgcttgcc cgatgatacc
     9661 atgtttacaa cattggatgg tgttgaacgc aaactaactt cacgcgatct gatgatctgc
     9721 gatgcagaaa aagggatgtg cattgccggt gtatttggag gtgaacacag tggtgtaagc
     9781 atggaaacaa aaagggtatt tcttgaaagt gcgtgtttca atcctgtgtc agtgcgtaag
     9841 actgccaagc atcacggatt gcataccgat gcttcgtttc gtttcgaacg gggaactgat
     9901 gcggaaatca ctatttacgc cattaaacgc gctgcattgc tgttgaagga actaacggga
     9961 tgcaacatca gttcagaaat ttcggattat tatccggtga aagtcgaaaa accggctgtt
    10021 gttctgaata ttgcatccat gaacgctttt gccggatcac cgattcctac cgacactgtt
    10081 gcaggtattt taaaagacct tgattttgta attacaaagc aaactccaga agagctaacg
    10141 attgaagttc cgctcaaccg caccgatgtt acaaggccag tggatgtata tgaagaaata
    10201 ttcaggattt acggctataa taatattccg atgccggaaa aatcaaatac aatttaccct
    10261 aaaaacacag gcatatcgct gaatgaatta atacaaaaag tatctgattt tctggctgca
    10321 aaaggattca gcgaaatcat gtgcaactcg cttaacagca tgcaatggta cgaaaaagca
    10381 agtggttatc cacttgaaaa tattgtaaac atccaaaatc cgctcagtcg tgaattgaat
    10441 gtgatgcgtt acagcatgtt gccggggatg cttcaatcgg cagcatacaa cattaaccgc
    10501 aatactgcta atctgaaatt tttcgaacca ggaaaaacat atgtgaaaaa tacaacagac
    10561 aaagagaaac cagttacagc tcggttttca gaaacaaaca tgctttcgtt actcatgacc
    10621 ggaagcgaac gtcctgaaaa ctggaaatac acagcagaaa catcggattt tttcagcata
    10681 aaatcggcgc tggaagatct ttttgatttt cttggcatta taaattccgt tgttgcaaga
    10741 acagaagtaa accatcagtt tacatttgga atgacataca tactcaacga aaaaacgctt
    10801 gcagccttcg gtctggtaaa aaacgagcta cgtacattat cagatgttga caatgaaatc
    10861 tggtatgcag agctaaatct tgatttgttg cttaagaaaa tccgtaaaac aaatatgcgc
    10921 tatcaggata ttccgaagta tccggcggtc cggcgcgatc tggcattact gattgacagc
    10981 aatagtactt ttgaaagtat tcagaaagcc gcactgggag ctgaaaagaa gctacttaaa
    11041 aaggtggatc tgtttgatgt ttacgtggat aaaaagctgg gtgacaataa aaaaagttac
    11101 gcaatcagct ttatgttccg tcacgatgaa aaaacgctga ctgatgcaga agttgataaa
    11161 gcgatggatc ggattacgac tgaggtgttg aaggtgaccg gcggtgtgat aagataaatg
    11221 aaataatcca tcgaagggtt ttgaaccctt cgaaagattg tgcaacttat cttccccgct
    11281 ctgcttcaaa agcaacggcc gcaatgcgac cacccaaagc cggaaaggtt ttatgaattt
    11341 tgcattttac atgttgcgcc aataaaaatt cagacaaaac agtatcaaga atatggctgg
    11401 cgacatgctc aataatgttg tggtgcactt ccataatctc tttaatgcgc agataaaccg
    11461 cctgataatt gattgttgtt tcaagtttgt cgctcaacat tgcctgtgaa gcgtcgaatg
    11521 aaatatacac atcaacagaa aagcccggat gtacgatttt ttcctcatcg aaacatccaa
    11581 ccggagcgcg gaattccata ccttcaattg aaataatctc cataaatagt ggctgataaa
    11641 aattttgctc gtaattttgt acaaaagtag aaaaaatggc cgacgaaaaa atcaatcttc
    11701 ctgaagaagg aaaagtgcag aaaaatttca ttgaagaaat cattgattcg cattacgaaa
    11761 gcggacggtt tgatcatgta ttgacccgtt ttccgccgga gcccaacgga tatctgcaca
    11821 ttggtcacgc aaaaagtatt tgtctgaatt tcgggctggc ccgcaaatac aacggcaaat
    11881 gcaatctgcg tttcgacgac accaatccga ccaaggaaga tgttgaatac gttgattcca
    11941 tcaaggaaga cgttaaatgg ctgggttttg agtgggccaa tgaattttac gccagcgatt
    12001 attttcagca attatacgac tgggccgaaa agctgattgc tgacggcaaa gcatacgttt
    12061 gtgaatgcag tcaggagacc attagtgcaa accgcaccac gcctacgcgt ccgggtattg
    12121 catgtgagca ccgcaaccgt acacctgaag agaatatgca gttgttccgc cgcatgcgcg
    12181 atggcgaatt tccggatggt tctatggttc tgcgtgccaa aatcgatctg aactcgccca
    12241 acatgcacat gcgtgatgcc atcatgtacc ggattctgca tgccgagcac catcgcaccg
    12301 gcaacaaatg gaatatttac ccgatgtatg attatgcgca cggacaaagc gattcgattg
    12361 aaggcattac gcattcagta tgcacattgg aatttgaagt acatcgcccg ttgtatgatt
    12421 ggtttgttgc caatctcggc attcatgcac cgcagcaaat tgaatttgca cgattaaatc
    12481 tgagctacac cgtcatgagc aagcgtaaat tgctgcagct ggtgaacgaa aagcatgtca
    12541 gtggatggga tgatccgcgg atgccgacca tcagcggtat gcgccgtcgc ggatttaccg
    12601 ctgaatctat tcgtttgttt gccgaaaaag taggtgttgc caaacgcgaa aacgtgattg
    12661 agcttggtct gctggaattc tgcgtacgcg aagatctgaa caaacgagcc gaacgtcgca
    12721 tggctgttct gaatccggtg aaactgatta ttacaaattt cgacgatacc aatattgatc
    12781 ttttaacggc catcaataat ccggaagacg aaacagccgg aacccgtgaa attccgtttg
    12841 gacgcgagct ctacattgag cgtgacgatt tcatggaaga gcctgtaaag ggctaccatc
    12901 gcctgtatcc gggcaatgaa gtccgtctgc gttatgccta ctttgtaaaa tgcaccggtt
    12961 gcaaaaaaga cgagaacgga aatgtgattg aggtatatgg agaaattgat ccggcgagcc
    13021 gtggcggcaa cagtcccgac ggccgcaagg tgaaagggac cattcactgg gtgcccgcca
    13081 aacaatcggt tgcctgcgaa acccggcttt acgaccggct tttcacagtt cccgcgcccg
    13141 atgacgctga agaaggaaaa acattccttg attatctgaa tccaaacagc atgaaaacag
    13201 tgacagcata ttgcgagcca gcgctgacag cggcaaagcc gggacagtat tttcagtttg
    13261 agcgattggg atattttgtt ccggataaag aaagtcctgc gggaaagccg gttttcaacc
    13321 ggacagtgac gctgaaagat agctggggga agtgatgatt taagannnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnntttga tttttgatcg gaagggcgat tgaaaaacga
    13501 actctgaaca ctgaacatag aacaaggaag ttcgaagtga gaagtaaaga ctaaactctg
    13561 aacatcgaac attcctacgc tatcggtagg aactttcagc tgaacaagga acctgtctga
    13621 cgccaggcag gcaaggaaga gaaaagaaag aaggatgttt tgatttaaga acattccgtg
    13681 ttatcaacgg aacaagagga tttttgattt ttgcctttcg acggagttta ctccgctgcc
    13741 tccttcgata cttcgcacgc tatttctcaa gaggcgctgc aggagcgaaa ctcaggatga
    13801 cagcgtgggt ctaagtgtga aaaaatttga tttttgaaat taatctgatt tgagattcga
    13861 attgcagaat ggaatgcaat acttcaaaaa ataaaaccgt atttcttatt caataatgat
    13921 tttagcagat tttctcgtca gctgggtgta gagggtcaca caataaacgc ctgatgcaaa
    13981 cggaagaaac agttcctgta gctgccggtc gtcggcctgc atataaaaaa cccgctggcc
    14041 caaaatgttt gtcacttcca ggttatagcc ctgtgaaaaa tcagagcccg ggtttacagt
    14101 aatgaagcca tcgctgggat ttggatatac tgcaaatgaa atatcaggta atgaatcgtg
    14161 tcttctgtac tcagagaatt tggccagaaa gcaattttcg gctccaatat ttatcagaga
    14221 gtcatttcca ataacaagaa cagaactggt ataagatcct gctatataaa tattgttttc
    14281 cttgtcaatg gtaagtgcaa aggcttcgtc aaatccctca tcaccagcac cgcggcaata
    14341 ttctgtttcg ccttcactgt tgtatttaag caataaaaag tcgggaaatg aatggccaac
    14401 caatgtatcg tttgcaataa aaagagtatc atacatgtat tgaccagtga taaaaatctt
    14461 tccttttgaa tcaaatacgc aattattgta atctgcaaca atgggatcat cgccgattga
    14521 tctggcccac aggaagtcgc ccgaagagct gatcatagcc gtaaacgcat ctgtattggg
    14581 tggaatgtat gtcaatgtgt cagagccgat aatcaacgga ttgctgttga aacaaccggt
    14641 gaaaacaatg ttcccttcat tgtcaatatc catgtcataa atattatcat gatcggttcc
    14701 ggcaatctgc ttcacatcac tatattcgaa gtcaggcgtc atttctatca caaatgcatc
    14761 cagagaaata cttggtggaa ttgtatccaa attgaaaatc gtatccggcc caattcccat
    14821 ccggggagtt gcgaagttgc cgcagaaaac gatgttgcca tcgggcttca ccttgattgc
    14881 tctgatgaca ataccatcgt ctgagtcttc gccgaaattg gtgccccaga ctgcttcacc
    14941 gttttcattc agtttaaaaa tgaacgcatt ggtacatggg tttccatttc cttttttgtc
    15001 cagcacaaca tcatccattg ataaaacata actgtcaaag ccgccggcaa taaaaatatt
    15061 atcattctgg tcggtgcaaa tgctgcttga gccgtcaaat gccggaccaa cagccgcttt
    15121 catccagatc aggccgctgg aaggactgat tttggcaaca aagaaatctc tcagacatgt
    15181 acagttcaca gcatagccat tataatacaa tgaaggactt tcaaaaactc ctgtaataat
    15241 gacattgcca tgtgaatcga ccgtcaatgc ggttgctttg ttgttgctgc cagccgaacc
    15301 ctgaaagctg tttgcccata tcacctgtcc tgaagaatcg aatttcacga tgaagatatc
    15361 cgacacaccg gtgttataca gagtagtatt gccaaatgtt atgcttgaac tgtaaaaccc
    15421 gcctgccatg taaacatttc catctgcatc ggttgcaagc gcattggcct tatcgacata
    15481 gtctccgcca aagccctttg cccaatgcca ctgaggcgtc tggcaatgaa tcagctgagg
    15541 aagtaaagat agcagtataa tggaaacagc aaaaatctta ttcatgataa ataaatttaa
    15601 tcaatgtaaa cagttccttt tatacaaatt taggaaaata attaaataga aaaatttctt
    15661 ttttgaattt cacattcaaa ctgcctttgc ttaataaaat tcagaatcaa ttcacaacgt
    15721 gtaaaacata tgtatgcgga gggcgcgatt cgacttcttt tgctgatttt cgctgcattt
    15781 ttgcccgttg tcattcagag tgtttgaacc aaatgcctca aaaacatccg acatcaaacg
    15841 tatcgaagaa cgacaacggg ccctgctgac tccttcgata cttcgcacgc tatgcgcaag
    15901 aggcgctgca agagcgaaac tcaggatgac agcagggttt tgttgaaaaa tcaataagcc
    15961 acataaatta ttcaacacaa acttatttac tttagctaat tcaacaaatt ataatatttt
    16021 tacaaacaat aatggtgaaa acaatccctg caatattgtc tacaatcctc ttgctgtcat
    16081 gtaacttttc caatccggaa aacaatgcaa atcactatgc attcagagat attaaaacct
    16141 gcttctttta taattattta ttagaaaaaa atccggcaaa ccttccatat ttcgaaaatc
    16201 aaattattca ggaactttca aaatgtataa accagaaaat actctttgaa gaactaattc
    16261 aagattccac atgctggctg gttctggaat gtatagactt taatacttcc cctgccggta
    16321 tccggcagcc aggatttgaa atttttcttg attcatgtgg cagcatgtct cataattgta
    16381 aaagtacctg tcccgaagaa cttgctgaca agtatatttc ataccttgac tggcgcagtc
    16441 aaaattttcg acgaatcaga ctaacagaca ttgcagacaa agaaatgatt ataagccctg
    16501 aaatgggtcg ggtattcaaa tccagaggat tccttgaaat gggaattgaa acaaacccgg
    16561 aaaattcagc acaaatcaaa caaataagaa atatcaggac ttttattcgt gaggttgctt
    16621 ccaaatacta tgatttcaga gacgaaacgg catacaagta tttctctaaa tcactcaaag
    16681 acctttcaaa aaccgaatgg ataaaaactg taaagttgtc agagttctat tctgagtttc
    16741 acttttttac gtcagatccg gattccttaa atcttttcct tattccctgt gatcacaaga
    16801 aactaataca aattcccaac agagaacacg ggattattga ctaatctctt gcaaaaaatc
    16861 tgcaccacca gatttcaccg aaatctccgt ctcagcacca acaaagaacg ctctgttcag
    16921 ttcagtatgt cccgccggaa aaccaaacag cagaggaaaa gaatattcac aaagctgatc
    16981 gcggataatg gtttccgcat cttttccaaa aggagtttcg ttatcatgca tctcgctcat
    17041 ggtgccgatc acaacgccag ccagattatc gaaaacgccg gcacgtttaa gtcccagcat
    17101 catacggtcg atgtgataca aatattcatc gagatcctcg agaaataata tttttccatg
    17161 catatccgga aatgaggcag agcctagcat tgaatacaac acactcaggt tgccacccac
    17221 aagaattc
//
LOCUS       DAOPKE010000060        17106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3536_length_17106_cov_10.834966, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000060 DAOPKE010000000
VERSION     DAOPKE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_17106_cov_10.834966"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKY63_08575"
     CDS             <1..1346
                     /locus_tag="PKY63_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00706.1"
                     /translation="PFAVTCSTGATGTVAFTSTDLFSSNTYTAQLSNASGSFASPVNI
                     GSLLTDANSGTITFTIPPGTPTGSGYMIRVISSNPVVIGSNSATFNVTLSGGPCSCYE
                     IESILVDACDEGVEGENEMFRFTVGGGDLNTSDIFIDWPNNPWKGVCQNAATDSIVAG
                     INATVTLPGQVVQPVGGVIPAGATVMFFTSTAFDFSKFDFSALNYTLYAVFQCEGNTQ
                     GHFGNHTSTGDKTLVLECGSGCHSETVTYNADSLYAGDGATVDFDVPGTPTYSDSGYC
                     ASVPIFDVLPIDLIFFKSICDGNSAVLYWSTATETNNAYFTIEAAESANADFVPVAKV
                     NGAGNSNEVLNYSVEVESSAKYFRLKQTDYDGKFTYSDVIAVDCEGSPVQLFPTLVAE
                     GENIIIIGEVSSIRVFDSMGREVVPEINENKVCCLEPGMYFFVINGKSNHKVVVQ"
     gene            complement(1921..2130)
                     /locus_tag="PKY63_08580"
     CDS             complement(1921..2130)
                     /locus_tag="PKY63_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00707.1"
                     /translation="MQINIKVKVRVEVKVKVKVKVKVKVKVKLLSAGKQPGCNFPDIL
                     SSVTTDMESSGAATAHKTKRTAVGQ"
     gene            2226..4442
                     /locus_tag="PKY63_08585"
     CDS             2226..4442
                     /locus_tag="PKY63_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00708.1"
                     /translation="MNKKFYALTCLIIASILTVHAQAPKAVTTMETFDAVSLPAGWTV
                     VDTLSDTHTWEFVTVYPVAYTLDGTPFAIVNSDAAGSVPMSEQLISPTYDLSSNNQIL
                     IEFDMYFHYFGGGGSESANAEVWDGSTWVNLMSITSDMGGWGTPQHVTIDASAHKNAN
                     FKVRFYYKGANGDYWWAIDNVKIWDPQPNDVGIASLVTPSNGFGMSAEAISVSVKNYG
                     TTAQTSIPVSYQVDGGAVVNETLNTSYTGFTTLAPGAEFTYTFTATYDFSAYQTYQIK
                     TWTGLATDTDHENDTLYTSISNAPELVQNTYDYILNGTLGIESDGDFFWVSFYTTPGK
                     FGKYSLSGTLLDTFRISSLAVGIRDLAYDPVSGHFFGGAGTPTIYELALDSVTPSLIG
                     SVSTPSGASVRYIAYDHVRDGFWVGNLNGDIFLVDRTGALLNATGIVNPIASGTLTGL
                     GNRYGLAFDDWSCPSEYLWIFSQPGTPSNVYLSQVDIYTGLPTGRSIDVTTQISFSGA
                     VPAAGGLFTHPDIVAGTVSVGGLVTGATTDADPNMIFIMDLQALHPDQPMVINSVFPA
                     PDSTNIQFGNVIELIINEPFVVNDTALIVITDGTTPVIIDSVTVSGDTLRIYHQDFSG
                     ETTYTVHFFAGALTGLCSDNTDFSWSFTTGVTGFTEAASGSVSLFPNPANNSIIVDGA
                     EGAVLKIFTLTGNCVLEQLLTEQNQQINTSNLVEGMYVIEIAAEGIRQLQKLVISR"
     gene            4907..5554
                     /locus_tag="PKY63_08590"
     CDS             4907..5554
                     /locus_tag="PKY63_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00709.1"
                     /translation="MTLKIEYGKHYHIFNRGNNKEILFYEEADFLHFLNLYSIYVLPV
                     TDTLSWCLMNNHFHFCVRIKEKEEIGFLNPEDLFKNKTSVKWKINKEQISSKYFKPTP
                     SRQFAHLFNAYSKWYNFKYSRSGSLFEKNFERSIVNNESYLVDLIVYINANPLKHRIN
                     NNAEDYVWSSAKGIINGSDILCNLPLIWNYFDDSDNFKQVINNCKWSADFWKDSE"
     gene            5957..7270
                     /locus_tag="PKY63_08595"
     CDS             5957..7270
                     /locus_tag="PKY63_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPF00710.1"
                     /translation="MGKTIIEKDIADSIKSKLGIKSAGQASIREIKKLADEIESATGV
                     KFVRMEMGVPGLPPAQAGIEAEIAALKSGVAALYPDIMGVKELKKEISLFVKNFLNIE
                     VSPEGCVPTVGSMQGGFASFMLLNRIKADRDTVLFIDPGFPVHKQQVKVLGNKFASFD
                     VYNYRGEKLREKLESMLSAGNIHSMLYSNPNNPSWICFTDTELKIIAEVAAKYDVVVI
                     EDLAYFAMDFRKDYSKPGQPPFQPTIANYYDKYILMISSSKAFSYAGQRIGMMVISDK
                     LFTSNYPDLKRFYSREEFGHAMIFGTLYSLSSGAAHTTQYGLLGILKAVNSGQYNFRD
                     DVKEYGEKAAIMKKMFTDNCFKIVYDMDENNPVADGFYFTVSYPGMSGDQLLDEFLYY
                     GISAIALSTTGSERTEGIRACVSLVHRNQFPALEERLKLFHQNHA"
     assembly_gap    7271..7370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7448..7975)
                     /locus_tag="PKY63_08600"
     CDS             complement(7448..7975)
                     /locus_tag="PKY63_08600"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HPF00711.1"
                     /translation="MSIKKPEEFLRNAVVDIAENMIIAAKTAPKGRGLERLSYLLITG
                     NELKIISHKMEELSKENKVPFLARDAGNLLKADALVLIGASYNPRAVQLCTHCGYTCD
                     KKPSEVPCAITMVDLGIAIGSAVSMAAHYHVDNRVMYSVGLAAVELGLFPKEIRSAFG
                     IPLSVSEKNIFFDRT"
     gene            complement(7987..8655)
                     /locus_tag="PKY63_08605"
     CDS             complement(7987..8655)
                     /locus_tag="PKY63_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00712.1"
                     /translation="MKIKADIGMRLPCFSLLIIIVLSIASCSSVVEEKYPDGTIKSEV
                     TMKNGRKNGPAKYYYPVGKVELSMNYQDDKLEGLYEKFTVKGIRTEMTMYSNGMKNGK
                     SEVFTEDGKILISALFINDTIHGPYKEYHPNGQIKVSGAYNHGLYHGDWEYYDATGIR
                     VGYAEFENGTGKQFALHYGSKRIKTEVNFVNNQKDGAEIWYDQNGNIDKKIMYSQGKI
                     ISTE"
     gene            8764..10782
                     /locus_tag="PKY63_08610"
     CDS             8764..10782
                     /locus_tag="PKY63_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPF00713.1"
                     /translation="MHFGAPSILWALLALAIPVIVHLFDFRRYRKVQFSDNYLLQQLL
                     EKNKRQSRLRRLIIMSLRMLFIAVLVLAFAKPYFPQSNEPLSSGRLFVSIYVDNSLSM
                     EVSDGKTDLLEKARSKAIEILNAYPPGTMFRLISNDFLGEHERFCPLDECADMLSLLD
                     FCPFPRKLSAVVSRMSDLSDMNASGAEHHFYIISDFQKSTADVALLASTDRKIFLFPV
                     DAPKTPNISVDSIWFSGPVQLENNLITVNARIRNHSDMLLEKIPVRLYVDGKQKGLGD
                     VELAASGSDVVEFTFTVNTRGSHEGYIEIDDSPISFDDRIFFAFDIASSVPVYHIAGS
                     GATKHINNLFERDSLFNFTSVREGSFDAGSMASAGLVILDEVSQISSGMASELNRLLE
                     SGGNILIIPAAGDIKNYQSGFAALGIPAFAGIDTANTRVASIEAENPLFRGVFVTPPK
                     QIQFPFVFNSYRIPKGNGRSIMTMLNGNSFMTDFTKGKGHIYLLSVPLQPTYSTFASN
                     ALIVPAFMQMAFMGRVMPGVAYSLDYSYGIDLPALVMSEEKPPVLTGFDGSSELIPGF
                     SNGTPPRIFLNSRISTPGAYKVMQGEKTFLSFGLNYPRSESNTACFSTDEIEQIVGGN
                     KKISVLATGATPAAVIADMQEGLKLWKTFIIFALIFFAIELILLRIWI"
     gene            10792..13413
                     /locus_tag="PKY63_08615"
     CDS             10792..13413
                     /locus_tag="PKY63_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPF00714.1"
                     /translation="MASEKDKKTEQGSNAQPEEKARITGLSGMYRNWFLDYASYVILE
                     RAVPNMADGLKPVQRRILHSMKELEDGRYNKVANIIGNTMKYHPHGDASIGDALVQLG
                     QKELLIDMQGNWGNILTGDSAAAPRYIEARLSKFALDVVFNPKTTKWIHSYDGRNREP
                     DFLPVKFPLLLAQGSEGIAVGLSTKILPHNFVELIDACIAVLKNETFAIYPDFPTAGL
                     VDVRNYNKGERGGKVRVRARITSPDKKSLVITEIPFSTTTSSLIDSIVNANDKGKIKV
                     RKIEDNTAANVEIVVHLAPGVSPDQTIDALYAFTDCEISISPLCCVIKDNEPRFINVE
                     ELLIDSVHHTRDLLKLELEIEMGELMEQHLYSSLEKIFIEKRIYRDIEECTTWESVLE
                     TIDKGLKPYKKKFYREITMDDIIRLTEIKIKRISKFDAFKADEYIKGLEEHIKKVQDR
                     LDNLIEYCIAYFRDIKKKYAKGRERKTEIRAFDNIEAAAVAIANQKLYVNREEGFAGF
                     GLKKDEYVTECSELDDIIAFTQEGSYIVTKVADKVFLGKNIIHIDVFRKNDERTIYNA
                     VYRDGATGTTYVKRFFVKGITRDKEYNVTQGTPHSKVLYFTANPNGEAEVISVYLVPR
                     PKLKKLVFDFDFSELAIKGRASIGNQLTRFMIRKIKLKDEGKSTLGGKKYWYDNEVMR
                     LSTDERGIYLGEFHHNDTFLFVMKSGYYRIAKPDTQMHFDEDLMHIEKFNPLRVFTVL
                     YKNADKEWYLKRFQVEQGPGEKTVEFMDEGDQIEWISTEYFPVITVFYDSKKLKRAKD
                     PEEIDVAEYAVIRGCKAKGKKISTVPLKKFSVNDPKPASDKMIRELLGLPDNQSTDPD
                     GNSETEEGLDGQITLSL"
     gene            13425..14642
                     /locus_tag="PKY63_08620"
     CDS             13425..14642
                     /locus_tag="PKY63_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPF00715.1"
                     /translation="MADNKTIQVSPKTTWIGVLDKEIVTFDVVMETKYGTTYNSYFIN
                     AEKKTVVETVKEKFWPVYKEKLSKLTALNEIEYIILNHTEPDHSGSVELLLNEAPNAV
                     VIGSGNAIRYMKDLMNRDFPHRIVKDGDVLDLGDATLKFISAPNLHWPDSMYTWYEEE
                     QLLFTCDSFGAHFAHDDMIDNKVGNYDDAFDYYFDVILKPYSKFFLKAIEKIEQLPIK
                     AILNGHGPLLLHDWKRYVDRSRSMSEAYLKNPDAKTILVAYVSAYQKTAMIAKEIARG
                     IESESNVKAHLMDIETATLGDIDAAITAASGLVLGSPTINQNILLPAYKFLAAICPIR
                     DKGKPAGAFGSYGWSGESAVLLKPMMENLKLDFCGDGVFVKFTPGNEELTLAFKYGKV
                     LAEKVLERDAASN"
     gene            14626..15447
                     /locus_tag="PKY63_08625"
     CDS             14626..15447
                     /locus_tag="PKY63_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00716.1"
                     /translation="MQLQTKLFLSVFLLSLTLLFSCQLEKKLARGFIADSTHPSLYIT
                     FSSDWEMFFIKPQGSSVSETYARRYFPYRSYFLKSNGTAGVDSIFKSSLNLSLQSNGF
                     DLYSDSTVNNFLIGSGDKFIIEFVQMYIEEKTLPVGDTLYFANNSFIQFDTVISSIDL
                     CFWLRINPVDDTLLASPLLYASFPLVDYFDGAWDYDLSSDRYIYNYNFTEFGETDIYM
                     LFENAGKKLAGYMYDYFLNQYLFKHMRRNPYAYYTWNGSFLRRAGQERFVFMKNP"
     assembly_gap    15635..15734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15893..>17106)
                     /locus_tag="PKY63_08630"
     CDS             complement(15893..>17106)
                     /locus_tag="PKY63_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPF00717.1"
                     /translation="ATLNATMDTVTLALSTNLINAVADTLYVQDVEDIAGNAMAAPQM
                     FVLMLDTVTIPKALVITEIMYNPAESGTDSTEFIEIYNNDVVTVDLMNYVLKYGSSTF
                     TFPSLVVNPGEYALVSSNAAAASNFYGVTFLQGSTGGIGNGGATVVLRSPSGLLVDTL
                     TYDDAAPWPTLADEGGYSLSLCDPDLDNSDGSNWSLGTVFFGTVNSLDVYADPGQGCV
                     TPSDTVPPVALHALLTNLSTAVVTFNEALDATSAETTSHYTGLGTVSSAVLSNDTVVT
                     LTLATALVNAQTYTLSVTGISDLASNVMTITYDFPLMLDTTTAITEMTNAAIQIFPNP
                     ASDVIHVSGTSTASHIEMYNNMGMLVLSREANGNESIIIETSSLANGSYYINIRMKDG
                     AVETRPVVIIK"
BASE COUNT         4627 a         3431 c         3827 g         5021 t
ORIGIN      
        1 ctccgtttgc agtcacctgt tcaaccggag ccacaggaac cgttgctttt acttcaacgg
       61 atttattttc atcgaacact tacacagctc agctatccaa tgcttcaggt agctttgctt
      121 caccggtaaa tatcggttcg ttgttaactg atgcaaattc tggtaccata acattcacca
      181 ttcctcccgg aacaccaacc ggctcgggat atatgatcag ggttatcagc agcaatccgg
      241 ttgttatcgg cagtaattcg gcaactttca atgtgaccct ttcaggcggg ccttgctcgt
      301 gctatgaaat tgaaagtatt ttggttgatg cctgcgatga aggagttgaa ggcgaaaatg
      361 aaatgttccg tttcacggtt ggtggagggg atctgaatac cagtgatatt tttattgact
      421 ggccaaataa tccttggaag ggggtgtgtc agaatgcggc cactgattct atcgttgccg
      481 gcatcaacgc cacggttact cttcctggtc aggttgttca gccggttggc ggggtcattc
      541 ctgcaggagc aacggtcatg tttttcacaa gtaccgcttt tgatttcagc aaattcgatt
      601 tttctgcatt gaattacaca ttgtatgcag tttttcaatg tgaaggaaat acccagggac
      661 atttcggaaa tcatacttct actggcgata aaactttggt tttggagtgt ggatctggat
      721 gtcactcgga aacggttact tataatgctg attcacttta tgcaggagat ggtgcaacag
      781 ttgattttga tgtgccgggt actccaacat attctgattc agggtattgc gcatctgttc
      841 ccatttttga tgttttacca atcgatctga tttttttcaa atcaatctgc gatggaaatt
      901 cagctgtttt atattggtcc accgctaccg aaaccaacaa cgcttatttc accatcgaag
      961 cagctgaaag tgccaatgct gattttgtgc cggtggcaaa ggtgaatggt gcagggaatt
     1021 ccaatgaagt gctgaattat tctgttgaag tagaatcttc agccaaatat ttccgcctca
     1081 aacaaaccga ttatgatggg aaattcactt attcggatgt gattgcggtt gattgcgaag
     1141 gcagtcctgt tcagcttttt ccaacgctgg tggctgaggg agagaatatc attatcattg
     1201 gagaagtaag cagcatccgg gttttcgaca gcatgggcag ggaagtggtc cctgaaatca
     1261 acgaaaacaa ggtctgttgc ctcgagccgg gcatgtattt ctttgtgatc aatggtaaat
     1321 cgaatcataa agtagttgtt cagtaatatt tatttacccc ccaattatca acaaagcgga
     1381 tctcctcaag gcggtccgtt tttgttttgg gtaaactctt tccactaaaa cacaacgact
     1441 gagttcccgg ccgaagcttg cagagtgccg ggatctcgcc gaaaaccgac acagcacctt
     1501 ggatttttct tccgcgaatt tggcagattt acgcgaagaa ttcttctttc ctcagcaggt
     1561 cttataaacg cgaacccgcc ggcatcagtc actattgaga catgatgggc ggacaaattc
     1621 gaatcagcct tcacctgtct gcaatttcaa ctgcaacagg cagtcaggaa ggcctgcgat
     1681 gaagcctgat tcagaggcgt acttagttcc tggtcccaga ttgagaaaca gctttgctga
     1741 ctgtgccggg atctcgccga aatccttcga cttgtcaggt tctacgtgaa gccctggctg
     1801 cgaccgctaa ggacttggtt gtactgggtg ggcggactgt tgggtttggg catggcagct
     1861 acttttccga cgattccata ttcgtagtaa cgggcgctaa aatgtcggga atcatcaaac
     1921 ttattgcccc acggcggtcc gttttgtttt gtgagctgtg gctgctcccg acgattccat
     1981 gtccgtagta acggacgata gaatgtcggg aaagttgcaa cctggctgtt ttcctgctga
     2041 aagcagctta accttaacct taaccttaac cttaacctta accttaacct taacctcaac
     2101 cctaacctta acctttatat ttatttgcat ttcacatcaa ttatatttat tattgcatca
     2161 tgtaaaacag aaacaacttc tcgttttgtt ttgctgtcta atgaaacaga aaagaaatct
     2221 tcactatgaa taaaaaattc tacgcactga cctgcctgat tatcgcatca atcctaactg
     2281 ttcatgctca ggcgcccaaa gctgttacta ccatggaaac cttcgatgcc gtttcgcttc
     2341 ctgcaggatg gacggttgtt gatacactca gcgacacgca tacctgggag tttgttactg
     2401 tttatccggt tgcctataca ttggacggga ccccctttgc cattgtgaac agcgatgcgg
     2461 ctggatcggt gcccatgagt gagcaattga tttcgccgac ctacgatctt tcttcgaata
     2521 atcagattct tattgagttt gacatgtatt ttcattattt tggcggaggc ggctctgaat
     2581 cagctaatgc agaggtctgg gatggaagca catgggtgaa tttaatgagc attacatcgg
     2641 atatgggcgg atggggaaca ccgcagcatg ttaccattga tgcatcagca cacaaaaatg
     2701 cgaatttcaa agtacgcttt tattataaag gtgctaatgg cgattattgg tgggctattg
     2761 acaatgtaaa aatctgggat ccacaaccca atgatgtggg aattgcatcg ctggttactc
     2821 cttccaatgg attcggaatg agcgccgaag caatttctgt ttcagtcaaa aattacggaa
     2881 caacggctca gacatcaatt cctgtttctt atcaggtgga tggtggtgcg gttgtaaatg
     2941 aaacgctcaa cacatcttac actggattta caacgctggc tccgggtgcg gaattcactt
     3001 atactttcac tgcaacctat gatttctcag catatcagac atatcaaatc aaaacgtgga
     3061 ctggccttgc aacggatact gatcatgaaa acgatactct ttacacatcc atatccaatg
     3121 ctccggagct agtgcagaac acgtatgact atatactgaa cggcacattg ggtattgaat
     3181 ccgatggaga ttttttctgg gtttcatttt atacgactcc cggaaagttc ggtaaatact
     3241 cgctttcagg cacattgctt gatacattta gaatttcttc attggctgta ggtatccgag
     3301 atctggctta tgatcctgta agcggtcact tttttggagg tgctggcacc ccaaccattt
     3361 atgagttagc tcttgattct gtgaccccat cgctcattgg ttccgttagt acgccttcag
     3421 gagcaagtgt gcgttatatt gcctatgatc atgtgcgcga tggtttttgg gtgggtaatc
     3481 tgaatggcga tattttcctg gttgaccgaa caggagcttt gttgaacgca acaggaattg
     3541 ttaacccaat tgctagtgga acactaacag gattgggaaa ccgctatgga cttgcctttg
     3601 acgattggtc atgtccttcg gaatacctgt ggattttctc gcaacccggc acgccaagca
     3661 atgtttacct gtctcaagtc gatatttata ctggccttcc gacaggacga agcattgatg
     3721 ttacaacaca aatatcattt tccggggctg ttcctgctgc aggcggactt tttacacatc
     3781 cggatattgt agcgggaact gtttctgttg gcggtctggt gactggtgca accacagatg
     3841 ctgatcccaa catgattttc atcatggatc tgcaggccct gcatcccgat cagcccatgg
     3901 ttatcaatag tgtttttccc gcacctgata gcaccaacat tcagtttgga aatgtaattg
     3961 aactcattat taatgaacca tttgtagtta atgatactgc actgattgtc attacggatg
     4021 gaaccacacc tgttattatt gacagtgtaa cagtttcagg cgatactttg cggatttatc
     4081 atcaggattt ttccggtgaa acaacttata ctgttcattt ctttgcaggt gctttaacag
     4141 gattatgcag cgataatacc gatttcagct ggtcatttac tacaggcgtt accggattca
     4201 ccgaggccgc cagtggcagt gtgtcattat tccccaatcc ggccaacaat tcaattattg
     4261 tcgatggtgc cgaaggagct gtgctgaaaa tttttacact cacaggaaac tgtgtgctgg
     4321 agcagctttt gacagagcaa aatcagcaaa taaacaccag caatcttgtt gaaggaatgt
     4381 atgtgattga gattgcagct gaaggtataa gacagttgca gaagctggta atcagcaggt
     4441 aattttaaga ttttcctcaa gttgatagtt gggtaaaaac aaatttctat agcataccga
     4501 ccttccagag ttcgcaatac aatggagaac ctggaagagt cgggacgctg aggacctctt
     4561 accaactatt ccatgttctc cgcggagttt atactgctcc gcagtgcttc gaactatgga
     4621 atgttggatt attgtggctt tttgaaaaat ttccccgacc ttccagagtc cgcaatgtta
     4681 tggagaacct ggaagagtcg ggacgctgag gacctcttac caactattcc atgttctccg
     4741 cggagtttat actgctccgc agtgcttcga actatggaat gttggattat tgtggctttt
     4801 tgaaaaattc tcccgacctt ccagagtccg cagtgtaatg gagaacctgg aagagtcggg
     4861 tttaagtaaa ttcaatatat ttgttcatag ataaaagaaa tgggaaatga ctcttaaaat
     4921 tgaatacgga aaacactatc acatcttcaa cagaggtaac aacaaagaaa ttttattcta
     4981 tgaagaggct gattttttgc attttctgaa cttatattca atttatgtac ttcctgtgac
     5041 tgacacgctt tcatggtgtc taatgaataa ccattttcat ttttgtgtac gtataaaaga
     5101 aaaagaggaa atcggttttt taaatcctga agacttgttt aagaataaaa cctctgtaaa
     5161 atggaaaatc aataaagaac aaatttcctc aaagtatttt aagccaacgc cctcccgcca
     5221 atttgctcat ctgtttaacg cttattcaaa atggtataat ttcaaatatt cacgttcagg
     5281 ctcattgttt gaaaagaatt tcgaaagaag catcgtgaat aatgaatcat acttagtgga
     5341 tctgatagta tatatcaacg caaacccatt gaagcacaga atcaataaca atgctgagga
     5401 ttacgtatgg tcatctgcaa aaggaataat taatggaagt gatattctgt gcaatttacc
     5461 attgatttgg aattattttg atgattcaga caattttaaa caggtgataa acaattgcaa
     5521 atggtctgct gatttttgga aagactcgga ataattacct tcaaaattat tgcatcatct
     5581 tccgaccttc cagagtccgc aatataatgg agaatctgga agagtcggaa gctgaggact
     5641 cttaccaact attccatgtt ctccgctttg cttcgaacta tggaatgttg gattattgtg
     5701 gctttttgaa aattctcccg accttccaga gtccgcaata taatggagaa cctggaagag
     5761 tcggaagctg ggacatctta ccaactattc catgttcttc gctatgcttc gaactatgga
     5821 atgttggatt gatttagttt gttttgagtt agtgtctaat atatatcaat ccgcaagttg
     5881 ttttgttcgt gtgagtattt atatgccata catcaaaaaa aatatttatt ttgtggcaca
     5941 ttaaatatat atcgtcatgg gcaaaaccat tattgaaaaa gacattgcag acagcatcaa
     6001 gtcgaaactt ggtataaaat cggccggaca ggcctctatt cgtgaaatca aaaagctggc
     6061 agatgagatt gaatcggcca ccggcgttaa attcgtccgc atggagatgg gcgttccggg
     6121 attgccaccg gcgcaggcgg gaattgaagc agaaattgcc gcgttgaaaa gcggtgtggc
     6181 agccttgtat cccgacatta tgggcgtgaa ggaactgaaa aaagaaatct cactcttcgt
     6241 gaagaatttt ctgaatattg aagtcagccc cgagggatgt gttcccactg tcggctctat
     6301 gcagggcggt tttgcatcgt tcatgctgct caaccggatc aaagctgacc gtgacactgt
     6361 tctgttcatc gatcccggat tccctgttca caagcagcag gtgaaggtgt tgggaaataa
     6421 atttgccagc tttgatgtat ataactatcg tggtgaaaag ctgcgcgaaa agctggaaag
     6481 catgttgtcg gcaggcaaca ttcacagtat gctctacagc aatccaaaca atccttcctg
     6541 gatttgtttt accgatactg aattgaaaat tattgcagaa gtggcagcga aatatgatgt
     6601 ggtggtgatt gaagacctgg cttattttgc catggatttc cgcaaggatt attctaaacc
     6661 cggccagccg ccttttcagc cgaccattgc gaattattac gacaaataca ttctcatgat
     6721 cagcagctcg aaagcattta gctatgccgg acagcgcatt ggcatgatgg tgatttcgga
     6781 caagctgttc acatctaatt atcccgatct gaaacgtttt tattcgcgcg aagaatttgg
     6841 tcatgccatg attttcggca ccttgtactc gcttagcagt ggtgctgccc ataccacaca
     6901 atacggactg cttggtattt tgaaagcagt gaacagcggc caatataatt tccgtgatga
     6961 tgtgaaagag tatggagaga aagcagccat catgaaaaaa atgtttaccg acaactgctt
     7021 caaaattgtg tatgacatgg acgaaaacaa cccagtcgcc gatggattct acttcaccgt
     7081 gagttatccg ggcatgtcgg gtgatcagct gctggatgaa ttcctgtatt atggaatcag
     7141 cgcaatcgca ctcagtacca ctggaagcga gcgtacagaa ggcatccgtg cttgtgtgtc
     7201 gctggtgcat cgcaatcagt ttccggctct ggaagagcgt ttgaagctgt ttcatcagaa
     7261 tcatgcataa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctcagagct
     7381 gattgctgat cactgactgc tgatcactgt aacgtgtaac gcttgtaacg cgtaacgttt
     7441 gtaacactta cgtccgatcg aagaaaatat ttttctcgga gacactgagc ggaatgccaa
     7501 atgcggagcg gatttccttt ggaaaaagtc caagctcaac cgcagccaga ccaacggaat
     7561 acattacgcg gttatcgaca tgatagtgag cggccatact caccgcgctg ccaatggcga
     7621 tgccaagatc gaccattgta atggcacagg gaacttcgga tggtttttta tcacaggtat
     7681 aaccgcaatg agtgcaaagt tgcactgcgc gtgggttgta cgatgccccg atgagtacca
     7741 gtgcatcggc tttcagtagg tttcccgcat cgcgggccag aaatggaacc ttgttttctt
     7801 ttgacaattc ttccattttg tgtgaaataa ttttcagctc gttgcctgta atcagcaagt
     7861 agctgagtcg ttccaggcct ctgcccttgg gtgctgtttt ggcagcaatt atcatgtttt
     7921 cagcaatgtc aacaacggca ttgcgcaaaa attcttcggg tttttttatg ctcatgcgga
     7981 tgatttttat tcggtggata ttatttttcc ttggctgtac atgatttttt tatcgatgtt
     8041 tccattttgg tcgtaccaga tttcggctcc gtctttttga ttgtttacga agtttacttc
     8101 tgttttgatg cgttttgacc cataatgcag cgcaaattgt tttcctgtgc cgttttcaaa
     8161 ctccgcataa ccgaccctga ttccggtggc atcgtagtat tcccaatctc cgtggtataa
     8221 tccatgattg taggcgcccg aaacttttat ctgtccgttt ggatgatatt ctttgtaagg
     8281 tccgtgaatt gtgtcgttga taaataatgc cgatatcagg attttgccgt cttcggtaaa
     8341 aacttcagat ttgccgtttt tcattccgtt tgaatacata gtcatttcag tgcgaatgcc
     8401 tttgacggta aatttttcgt acaatccttc gagtttgtcg tcctgataat tcatactcag
     8461 ttctaccttt ccgacaggat agtagtattt tgctggtcca tttttgcgtc cgtttttcat
     8521 ggttacttca gacttaatgg tgccatcggg gtatttttct tccaccactg aagagcagga
     8581 agcaattgat aatacaatga ttattaacaa tgaaaaacaa gggagacgca taccaatgtc
     8641 ggcttttatt ttcaaagata acaatcttta tccaattgtt gttcatcagc ccgcgaaggg
     8701 tttcaaacac ttcgcgggct ggagtctctg aattcgcatt aataattacc tttgcaatga
     8761 attatgcatt tcggagctcc ttccatattg tgggcattgt tggccctggc tatcccggtt
     8821 attgtccatc tgttcgattt cagacggtac agaaaagttc agttttcgga caattacctc
     8881 ttgcagcaac tgcttgaaaa aaataagcgc cagagccgtt tacgacgtct tattattatg
     8941 agtttacgta tgcttttcat tgctgttctg gtattggctt ttgcaaagcc atattttcca
     9001 caaagtaacg aacctctgtc ttccggacgt ttgtttgtta gcatttacgt tgacaacagt
     9061 ttgagtatgg aggtcagcga cggcaaaact gatctgctgg aaaaagcaag atccaaagcc
     9121 atcgaaattt tgaatgccta tccgcccgga actatgtttc gtttaatcag caatgatttt
     9181 ctgggtgagc acgaacgttt ttgtccgctt gatgaatgtg ccgatatgtt gtcgctgctt
     9241 gatttttgtc cttttcctcg caaattaagt gctgttgttt ctcgcatgtc cgatttgtct
     9301 gatatgaatg cttctggcgc agagcaccat ttttatatca tcagtgattt ccagaaatca
     9361 acagcagatg tggctttgct ggcttcaaca gatcgaaaga tttttttgtt tccggtggat
     9421 gcgcctaaaa caccgaatat ttcggttgac agtatctggt tcagtggtcc ggtgcagctt
     9481 gaaaacaatc ttatcacggt caatgcacgt attcgcaatc acagcgacat gttgcttgaa
     9541 aaaattccag tccggctcta tgtcgatggc aaacagaaag gacttggcga cgtagaactg
     9601 gctgcttcgg gctctgatgt tgttgaattt acctttaccg taaacactcg tggaagtcac
     9661 gagggatata ttgaaattga tgacagtccc atcagtttcg acgatcgcat tttctttgct
     9721 tttgacattg cttcttcggt tccggtttat catatcgccg gtagcggagc aacaaagcat
     9781 atcaataatc tgtttgagcg cgacagtctt ttcaatttca cttcagtgcg cgaagggagt
     9841 tttgatgcgg gttctatggc ctcggccggg ttggtgatcc ttgatgaagt gtcgcagatt
     9901 tcttcaggaa tggcttcaga attaaatcga ttgcttgagt cgggtggcaa tattctgatt
     9961 atacctgcag ccggcgatat caaaaattat cagtccggat ttgcggcact tggcattcct
    10021 gcatttgccg gcattgatac ggccaataca cgtgtagcca gtattgaagc tgagaatcca
    10081 ttattcagag gagtatttgt aacaccacca aaacaaattc agtttccgtt tgtattcaat
    10141 agttaccgaa tcccgaaagg aaatggaaga agtatcatga ctatgttgaa tgggaattca
    10201 tttatgaccg attttacaaa gggcaaagga cacatttatt tgttatctgt tccgcttcag
    10261 ccaacatatt ccacctttgc ctcaaacgcg cttattgttc cggcttttat gcaaatggct
    10321 tttatgggca gagtcatgcc gggggtagct tactcacttg attatagtta tggcattgat
    10381 ttaccggctt tagtcatgag cgaagagaag ccacctgtgc ttacaggttt tgatggatca
    10441 tccgagctca ttccggggtt cagcaacggg actccaccca gaatttttct taattcgcgc
    10501 atcagcactc ctggcgcata taaagttatg caaggtgaaa aaactttttt atcatttggt
    10561 ttaaattatc cgcgtagtga aagcaataca gcttgtttca gcacagatga aatcgagcag
    10621 attgtcggcg gaaataaaaa aatatcggtg cttgcaacgg gcgctacacc tgcagcggtt
    10681 attgccgaca tgcaggaagg attgaaactg tggaaaacat ttatcatatt tgctttgata
    10741 tttttcgcca ttgaattaat tttgctgaga atctggattt aatgtttaac gatggcttct
    10801 gaaaaagata aaaaaacaga acagggctcc aatgcacaac ccgaagaaaa agccaggatt
    10861 actggtttgt cgggtatgta ccgcaactgg tttcttgatt acgcttccta tgttattctg
    10921 gaacgcgctg ttcctaatat ggcagatgga ctgaaacccg ttcagcggcg gatcctgcat
    10981 tccatgaaag agctggagga cggtcgttac aacaaggtgg ccaatatcat cggcaacacc
    11041 atgaagtatc atccgcatgg cgatgcttct atcggtgatg cactggttca gcttggacaa
    11101 aaagagttgt tgattgatat gcagggaaac tggggaaata tcctcaccgg agactccgct
    11161 gccgcacccc gatatataga ggcaagactt tcaaaatttg cactggatgt agttttcaat
    11221 ccgaaaacaa ccaaatggat tcattcttac gatggccgca acagagaacc tgactttctt
    11281 cccgtgaaat ttccgctgct gctggctcag ggctccgagg gaattgcagt cggactttcg
    11341 acgaaaattc ttccgcacaa ttttgttgaa ctcatcgatg catgcattgc tgtgttgaaa
    11401 aatgaaacat ttgccattta tcccgatttt ccgactgccg gactggttga tgtcagaaat
    11461 tacaataagg gtgagcgtgg agggaaagtc cgtgttcgtg caagaatcac ttcgcccgac
    11521 aaaaaatcac tggtcattac tgaaattcct ttttcaacaa cgacttcttc attgattgat
    11581 tctattgtca atgccaatga taaaggaaag atcaaagtcc gcaaaatcga ggataatact
    11641 gcagccaatg tagaaattgt ggtgcatctg gctcccggtg tttcgcccga tcagaccatt
    11701 gatgcgttgt atgcctttac cgattgcgaa atttcaatct ctccgctttg ttgtgttatt
    11761 aaagacaatg agccgcgttt tatcaatgtt gaggaattgc tgattgattc ggttcatcat
    11821 accagagatt tgctcaaact ggagcttgaa attgagatgg gcgaattgat ggagcagcac
    11881 ctgtattcgt cgctcgaaaa aatatttatt gaaaaacgga tttatcgcga cattgaagaa
    11941 tgtacaacat gggaaagtgt tcttgaaaca attgataaag ggcttaaacc atataagaag
    12001 aagttctatc gcgagattac aatggatgac atcattcgtt tgactgaaat aaaaatcaag
    12061 cgaatttcga aattcgatgc ctttaaagct gatgaataca tcaaaggact tgaagaacat
    12121 atcaaaaagg tacaggatcg cctcgacaat ctgattgaat attgcattgc ctatttccgc
    12181 gatattaaaa agaaatatgc aaaaggtcgt gaacgtaaaa ccgagatccg tgcattcgac
    12241 aacatagaag cggcagctgt agctattgcc aatcagaagt tgtatgttaa ccgcgaagaa
    12301 ggatttgcag ggtttggcct taaaaaggat gaatatgtaa ctgaatgttc tgagcttgac
    12361 gacatcattg ctttcaccca ggaaggatcg tatatagtta ccaaagtagc cgataaggtt
    12421 tttttgggta aaaatattat tcacattgat gtgtttcgta aaaacgatga gcgcaccatt
    12481 tacaatgctg tttatcgcga cggtgcaacc ggcacgactt atgtgaagcg gtttttcgtg
    12541 aaaggaatta ccagagacaa agaatataat gtaacgcagg gaacacctca ttccaaagtg
    12601 ctttatttta cagctaaccc aaatggtgaa gcagaagtga tttcggtgta tcttgttccc
    12661 agaccaaagt tgaaaaagct ggtatttgat tttgatttca gcgaactggc tatcaaaggt
    12721 cgtgcatcta ttggcaatca gcttacacgg tttatgattc gtaaaatcaa gctcaaagat
    12781 gaagggaaat cgacattggg gggcaagaag tattggtatg acaatgaagt gatgcgtctt
    12841 tcaaccgatg aacgtggaat ttatctgggt gagtttcatc acaacgatac ctttcttttt
    12901 gttatgaaat cgggctatta ccgcattgca aaacccgata cgcagatgca ttttgatgaa
    12961 gatctgatgc atattgaaaa attcaatcct ttgcgcgtat ttactgtttt gtataaaaat
    13021 gctgacaagg aatggtatct caaacgcttt caggtcgagc agggccctgg cgaaaagact
    13081 gttgaattta tggacgaagg cgatcagatt gaatggattt cgacagaata tttccctgtg
    13141 attactgttt tttacgattc aaaaaaactc aaaagggcca aagatcccga agaaattgat
    13201 gtggctgaat atgcagttat tcgtggttgc aaagccaagg gaaagaaaat aagcacggtt
    13261 cctttgaaga aattctctgt gaacgatccg aaaccggctt cagacaaaat gattcgcgaa
    13321 ttgctcggat tacccgataa tcagagtaca gaccccgatg gaaattcaga aactgaagaa
    13381 ggactggatg gacagataac tttatcactt taaaaaataa aagtatggct gacaataaaa
    13441 caattcaggt atctcctaaa acaacatgga tcggtgtact cgacaaagag attgttacct
    13501 tcgatgttgt tatggaaacc aagtatggta ctacctacaa ctcatatttc attaatgctg
    13561 agaagaaaac tgttgttgaa accgttaagg aaaaattctg gccggtgtat aaagaaaagc
    13621 tcagtaaact aacggcgttg aatgagattg aatatatcat tcttaatcat acagaacctg
    13681 atcatagtgg ttcggttgag cttttgctga atgaagcacc caatgccgtt gtaattggca
    13741 gcgggaatgc cattcgttat atgaaagatc tgatgaaccg cgattttccg catcgtattg
    13801 tgaaagatgg tgatgtgctt gacctgggcg atgccacact gaaatttatc agtgcgccaa
    13861 atctgcactg gcccgacagt atgtacacct ggtacgaaga agaacagcta ctgtttacct
    13921 gtgattcgtt tggtgctcat tttgcacatg atgatatgat tgataacaag gttggcaact
    13981 acgacgatgc ttttgattat tatttcgatg ttattctcaa accttacagc aaatttttcc
    14041 tgaaagcaat tgaaaaaata gagcaactgc ctattaaagc cattttgaat ggtcatggac
    14101 ctttgttgtt acatgactgg aagcggtatg tagatcgttc cagatcgatg agcgaagcgt
    14161 atctgaaaaa tccggatgct aaaacaattc ttgttgccta tgtttctgct tatcagaaaa
    14221 cagcaatgat tgcaaaggaa atagcccgtg gtattgagtc tgaaagcaat gtcaaagctc
    14281 atttgatgga tattgaaact gcaactctgg gtgatattga tgcagccatt acggccgcca
    14341 gtggactcgt tctcggtagt ccgaccatta atcagaatat tcttcttcct gcgtataaat
    14401 ttctggcagc gatttgtccc atacgcgaca aaggcaagcc ggccggcgct tttggttcgt
    14461 atggatggag cggggaatcg gcggttttgc ttaaacccat gatggaaaac ctgaagttgg
    14521 atttttgcgg tgatggtgtg tttgtcaaat ttacacctgg gaatgaagag ctaactcttg
    14581 cattcaagta tggtaaagtt ttggctgaaa aagtgctgga gcgtgatgca gcttcaaact
    14641 aaactttttt tatctgtctt tttattgtct ctgacattgc tcttttcctg tcaattggag
    14701 aagaagctgg cgcgtggttt tattgcggac tcaacgcacc cgtcgctgta catcacattc
    14761 tcgtccgatt gggaaatgtt ctttattaaa cctcagggct cttctgtttc tgaaacatat
    14821 gccagacgct attttccata ccggagctat tttttaaaaa gtaatgggac tgctggggtt
    14881 gacagcattt ttaaaagttc actgaatctc tcgcttcaat caaatggatt tgatttgtat
    14941 tcggatagta ctgtcaataa tttcctgatt ggcagcggtg acaaattcat tattgaattt
    15001 gtacagatgt atattgagga aaagacactt ccggtcggcg atacattgta ttttgccaac
    15061 aatagtttta ttcagttcga taccgttatt tccagcatcg atctttgttt ctggctccga
    15121 atcaatccgg tcgatgacac tttgctagca tctccgttgc tgtatgcatc gtttccgctt
    15181 gtagattatt ttgatggagc atgggactac gacttgtctt cggatcgata tatctataat
    15241 tataatttta cagaatttgg tgaaacggat atttacatgc tgtttgaaaa cgctggaaag
    15301 aaactggccg gttacatgta tgattatttt ttaaatcagt atctttttaa acatatgcga
    15361 cgcaatccat atgcctatta cacatggaat ggcagttttc tgcgcagggc agggcaggag
    15421 cggtttgtgt ttatgaaaaa cccgtgatga tataagatat cagattgtag atttttgatt
    15481 tttgatgtta agggtgaagg aacattgaac actgaacatc gaacactgaa attagaattg
    15541 gcagctaaat gtagaaaaag cttctttgcg tattcagcca ggttcactgc tcaactgtca
    15601 ccctgagccc tgcattttgc aggataaacc ccggnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnctcaac ctgggcgcgc gtctcccgct tgcaaccacg taaaacagct
    15781 tgtctcgttt tacgtgatgg caagctgttt tacgttgacg cgtgacctta atcttgacta
    15841 ataaaaaaaa ggccatccgc atggacagcc ttttttggaa gaaataacct gattatttga
    15901 ttatcacaac aggtctggtt tcaacagcgc cgtctttcat gcggatgttg atgtagtaag
    15961 aaccattggc aagtgaagaa gtctcaataa tgattgactc gtttccattt gcttcgcggc
    16021 ttagaaccag catacccatg ttgttgtaca tttcgatgtg gctggctgtt gatgttcctg
    16081 acacatggat gacatcgctg gccgggttcg ggaaaatctg aatagcggca tttgtcattt
    16141 ctgtgatggc agttgtagtg tcaagcatta acgggaaatc ataggtgatg gtcattacat
    16201 tgctggccag atcgcttatt ccggtaacgg aaagtgtgta ggtctgtgcg tttacgagtg
    16261 cagtagccag tgtgagagta acaacggtat cattgctcag cacagctgaa gaaaccgtgc
    16321 ctaatcctgt gtaatgtgaa gtggtttcgg cagaagttgc atcgagtgct tcgttgaaag
    16381 ttacaacagc tgttgaaagg ttggtaagca acgcatggag tgcaacagga ggaacggtgt
    16441 cggaaggtgt tacacatccc tgacccggat cggcataaac gtcaagtgag ttcactgttc
    16501 cgaaaaacac tgtacccagg ctccagttgc tgccatcgct attgtcaaga tcaggatcgc
    16561 aaagcgacag actgtatccg ccttcatcgg caagggttgg ccatggggct gcgtcatcat
    16621 aagtcaaagt atcaacaagc aatcccgagg ggctgcggag aacaacggtt gcgccgccat
    16681 taccaattcc accagtactg ccctgcagga aagtaacgcc gtagaagttg ctggcagcag
    16741 cagcgttgga tgaaacaaga gcgtattcgc ctggattaac tacaagagat gggaaggtga
    16801 aagtgctgga accatattta agaacatagt tcatcagatc aacggttaca acatcattat
    16861 tgtagatttc aatgaattca gtactgtccg ttccactttc agcggggttg tacatgattt
    16921 cagtaatcac cagcgctttt ggaatagtca cggtgtcaag catcagtaca aacatttgcg
    16981 gggcagccat agcattgcca gcaatatctt cgacatcctg aacataaagc gtatcagcaa
    17041 cagcattgat aagatttgtt gaaagagcaa gagtaacagt atccatggtt gcattgagtg
    17101 ttgcgg
//
LOCUS       DAOPKE010000061        16909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3597_length_16909_cov_13.974012, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000061 DAOPKE010000000
VERSION     DAOPKE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3597_length_16909_cov_13.974012"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..3392
                     /locus_tag="PKY63_08635"
     CDS             300..3392
                     /locus_tag="PKY63_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPF00718.1"
                     /translation="MDYNHRDTEARWQKYWADHKTYRTEIVPDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFARYKKLKGFNVLHPMGYDAFGLPAEQYAIETGQHPAITTDV
                     NIKRYRSQLDKIGFSFDWDREVRTSDPAYYKWTQWTFIQLFHSWFDKSQQKARPIKDL
                     ITLFEDSGNENIPAAHSCDVIFTGSEWNVMSEEKQSDILMDYRLAYLADTMVNWCPAL
                     GTVLANDEVSEGFSVRGGHPVERKEMKQWSLRITAYADRMLEGLERIEWPENIKEIQR
                     NWIGRSEGAMLRFLLADHPDKHLEIFTTRPDTVFGVTFMSVAPEHEYVELLTKPEQKA
                     VVNAYVKHAKNRSERERMADAKTPSGVFTGSYAINPFNGKAVPVWVADYVLSGYGTGA
                     VMAVPAHDSRDYAFAKHFGLPIIEVIKGGNIEEEAFESKEGVCVNSGFIDNMIARDAI
                     KKVIAHAEEKGFGYGKINYRLRDAIFSRQRYWGEPFPIYYKNGIPHTIDESELPLQLP
                     EVDKYLPTETGEPPLARAKNWHTKAGHPYETSTMPGFAGSSAYYFRYMDPRNDENYFS
                     KDAVNYWKSVDFYVGGAEHATGHLMYARFWNKFLFDLGLTPEDEPFRKMINQGMIQGR
                     SNFVYRVKAERMAEFVLWEMIRNKQLGAEFKKGYTQGEFSFDFYSPESHIIIEVKSSH
                     DVLERLPNCAEFAEKNNCQLLMFSIVDVILHTEDVLKKIKKALSGKDVSPFEMIDEWE
                     EAPIFASKNIFGREQFSFPVHVDVNIVNNDILDIDAFRAWRPEYNNARFILEEDKYVC
                     GYEVEKMSKSKYNVQNPDDLVERYGADTLRMYEMFLGPVEQSKPWDTNGIEGVSRFIR
                     KLWKLFVGEEDNLLITDDTPTAAELKTLHKTLKKTEEDIERFSLNTCVSTFMICVNEL
                     SDLKCRKRAILEPLLIALCPFAPHISEELWNRLGNTETISRATFPTWDEKYLIEDTVK
                     YPVSFNGKMRFILEVAAGTAKEEVEKLALSAPDSQKWLEGKAPKKIIVVPGKIVNVVI
                     "
     gene            complement(3747..4736)
                     /locus_tag="PKY63_08640"
     CDS             complement(3747..4736)
                     /locus_tag="PKY63_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPF00719.1"
                     /translation="MKIYSFLFLFCSFAAFAQTTFSFDDSLRTNRIMYDISLLAHDSL
                     MGRDGGSVYEMRAGDYIIAEFIECGLIPLQGTDHFRQEFVLRKITNEKGDTIVVKSNN
                     ILGYIENNAPYTIVIGGHYDHIGYYYKDSALYINNGADDNASGAAGVMEMARYLSSGN
                     LTKYNYIMACWGSEEQGLVGSNYFCSSNLFPFEQIAFYINFDMIGRLGWKSDQLDIYG
                     LGTAMVWDSLMPETEYGKYKLKKFAGGVDASDHSCFYKNKTPYIYFTSGLPPVYHTPK
                     DETPLINPQGVELIIKYTEDIISRFDGSKPAFRKVSQKEMNKVYWYFLSQFMN"
     gene            complement(4766..5416)
                     /locus_tag="PKY63_08645"
     CDS             complement(4766..5416)
                     /locus_tag="PKY63_08645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPF00720.1"
                     /translation="MKFPYHFITVEGCIGAGKTSLSTRLAADFNGKLIVEQFEENDFL
                     PKFYKNPERYAFPLELSFLAARFKQFKDNVLNADIFYDYLISDYIFPKSLIFSRKTLQ
                     DDEYRLYKNLFQIIDSNLPRPDLVLYLHLPPEKLKENIIRRGRGYEQDISLEYLDNIQ
                     KSYFEYFHEQSSLRVVVADTSNLDFVANNDHYNFLVSLLTQEYKPGITVVPPFSSL"
     gene            complement(5426..5929)
                     /gene="folK"
                     /locus_tag="PKY63_08650"
     CDS             complement(5426..5929)
                     /gene="folK"
                     /locus_tag="PKY63_08650"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPF00721.1"
                     /translation="MNRHNIFLSLGSNEGNREWLLQQAIDMIRSYIGEVVRVSNIYEG
                     EPIGFKSEKQFLNLCLEMKAEGSPHQLLLKLEAIEVKLGRLKPSIFSEYTDRTIDLDI
                     VFFDDLVVNTEELTIPHPKMHLRRFVLEPLAELCPDFIHPVLKKTVKVLLGECPDESK
                     LTVTLKR"
     gene            complement(6030..6695)
                     /locus_tag="PKY63_08655"
     CDS             complement(6030..6695)
                     /locus_tag="PKY63_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF00722.1"
                     /translation="MRDDYLNRQIIERVLSSVRALFPMDGSGHGFDHVLRVYHMATRI
                     AEEEGCDVFIVSLAALLHDVGDHKLTADGNENHRNAIFALLNETGVTDDQTHQIIDIV
                     EKVSFKGNGVDDAPMSAEGCCVRDADRLDAMGSIGIARAFAYGALKGRPMFDDGQKPA
                     LHENFSDYKNSKSHTINHFYEKLLLLKDRMETDAGKRLAQGRHAMLEAFLSEFLLEWK
                     AQK"
     gene            complement(6688..7536)
                     /locus_tag="PKY63_08660"
     CDS             complement(6688..7536)
                     /locus_tag="PKY63_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00723.1"
                     /translation="MMKTITFLVFIAAIAIAGNAQDTVFTVRVFEKKNLVERRSYGIH
                     GNLLAKETCLSNDPLLWFREFMIYDKGKLIRSGHYINESDVTDYSYSYNDKGKLEKKR
                     ESQKGIVSGETFYKYNEKGWLVEEEAYHGKRKKIVTVYEYTPEGLVKKRLENISDSNQ
                     IMILSELYFYNTDGKMLRVVGLNGEDTISDIRYTYDKFGFKLRQDVISHGDTTLTVKW
                     KYGRNGSRPVLEQHWADGYVVRETKNFFTSKGRIKKEVIVEYRIYSGKMKPEKTIRKY
                     RYFWNA"
     gene            complement(7533..11978)
                     /locus_tag="PKY63_08665"
     CDS             complement(7533..11978)
                     /locus_tag="PKY63_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPF00724.1"
                     /translation="MSKISKKTGRILLIIYVVVFCLPAAVFALLRTPLIQQYIANRLS
                     EYLSEELHTKVEIGTVDITFPVDITLCNLYIEDQHGHPLLAAKELLVAPENLASGFSK
                     LQFEKLLLNEARIRMVRYPDEDKTNLQFILDYFAGEEKDTSTKKTESPPVSVGMLELR
                     NCSFFWQDMRKDTSAKNNIDFNNLYLTNLNLIGQNLYQCDTLTSIDLKHLAMQDRSGF
                     TLWHLSSQVSMRKNSITAQKLRFLTNSSNLDLDLVFRYDSISAFSDFVNKVSLDADFR
                     NSRVGFDDIARFVPAMNGMRSVVNLNGHAGGTIADLETGQMKLGFGKSSHMYFNLKLI
                     GLPDINNAEVHFDLVNADLKLADIDAFVLPGGNTLKLASVARSVNQANLSAQIDGKLN
                     DFSANIVAKTNSGFVKADIQSQGEFPNPSYSGNIVVENFDLKTFAGESVPVGALTASV
                     DFSGSGTSATRYFIEGGAEILSLEYNNYLYSDISVNGSVKPGIFEGQLLASDPNLSLD
                     FDGMIDFSEKIPVFDFIAQVENIDLSALHLARNDSLAGFSGFIDINVIGNHPDNMLGR
                     LVLNDFSYYEGPNEIDMNSLSLKLEKTDSVNKVIELRSDLVNGNIKGQFVFSEIDDAF
                     NKYLSNYIPAYIVPDSSAKKLSNQAFEFEFTFRDVQPVLDIFAKGIQISENTSLHGNF
                     NLEENFLNTQIASNYLKSGAISSVNPVVIAQTFGKNIYVTVQADNLTYNDSLELGNLV
                     ANTVTVNDKSDFSINWRNDNSTKAYSGDLSGNVLFRKGMPVLIQFDESDLTLNDSLYH
                     IAENGKIEIDTGYLRIHEFALYSKSQKLMIDGRISKDPYDVVQVSFENVLLDDFDDLT
                     HSSNIDLDGKVNGYVLLSNLYDVPDYRADVTVQKLSVNKNFVGDALLKSAWDPLRKAV
                     YSEASIVYTGNVGQNVPLEIKGFFNPRDKENMLDLVVKLDRFNLKLVQPYLKSFCSRV
                     EGSCDGLVLVKGNIDKPDVSGEVVFRRTSVLVDYLNIFYSFADTLKINNSEFALRDIT
                     VFDTKGNKATGNILATHDYFKNFYLDINLRPEKMQFLNTTAKDNDYFYGTAYASGVVK
                     IHGPFNQIAIEVVAKSEPGSVLYIPITSSTEVYENDFITFVSNKNDSSVVQKDVKIRD
                     SGVSLMFDIDVTPDAEVQIVFDPKIGDVMKGRGEGRLRMTIDRSGEFLMFGNLEIEKG
                     DYLFTLENVINKKFLVDRGGVITWNGDPYAGKMDLYARYSIKTSLYELVSVADESELY
                     KNKVPVSCLMHLTGDLLSPDISFDLDLPESDENTKNLVRTVIGNAEEMNRQVFALLIL
                     NSFIPTERNTFNSPISQGLGNTSIELISNQFGNWLSQISNDFDVGFSYNQGDQVTSSQ
                     VEIALSTQILNDRIVLETNLEIGGNQLGSPDNQQASNIAGDVSIEYKISEDGKIRIKA
                     FNRSNTVDVVANNAPYTQGVAIFYRKDFDKFRELWERKKKTKINK"
     gene            11985..13007
                     /gene="tsaD"
                     /locus_tag="PKY63_08670"
     CDS             11985..13007
                     /gene="tsaD"
                     /locus_tag="PKY63_08670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPF00725.1"
                     /translation="MHTYILGIESSCDDTSAAVLQNNFLLSNEIASQKVHERFGGVVP
                     ELASRAHQQNIIPVVEAAISHANIDKKQLSAVAFTRGPGLPGSLMVGVSFAKAFAWAL
                     GIPLIDVDHMHAHIMANFLRVPGETRSMPEFPFLCLIVSGGHTQIAIVRNPLEREIIG
                     NTIDDAAGETFDKAAKIMSLPYPGGPVIDKLAQSGNPSAFRFAKPRIADLNFSFSGLK
                     TSILYFLRDELKKDEQFIEKNTADLCASIQKTIIDILVDKTEKAVNGTGIRTLALAGG
                     VSANSVLRAAMRQLADKHKLDLHIPKLSYTTDNAAMIAMAGYFRYQKGLFAGLDAAPY
                     ATGVKV"
     gene            complement(13239..13844)
                     /locus_tag="PKY63_08675"
     CDS             complement(13239..13844)
                     /locus_tag="PKY63_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00726.1"
                     /translation="MNNLPRLLSWLLHPIILPALFAVLALNGDYYLNNMLRPEAVRII
                     ILVVLIFTLVIPALIFVASRYLGVIESLEMEIKQERIFAVTVIGISAWFCWWILSNYE
                     LPAYYTDFLLLIFSASAFGLIVSFFKKFSLHIFGWSVIVAAIAWYIFSWQCFSVLYLA
                     LAVVLTGVVAWSRLKLNAHTPGEIYLGFGLGLLPVVVLVIL"
     gene            complement(13894..15372)
                     /gene="rpoN"
                     /locus_tag="PKY63_08680"
     CDS             complement(13894..15372)
                     /gene="rpoN"
                     /locus_tag="PKY63_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPF00727.1"
                     /translation="MLHQRLEQKLQQKLSPQQIQLMKLLQVPAIALEQRIKQEIEENP
                     ALEDTSEDNEDDLNQDLQDEEQERDEKEDSDIEENVENDFSLDDYMDEDDIPEYKLAA
                     KNTSADDEVREVPFVSQSSFYDQLYEQLQMRDLNELQYRVGEYIIGNIDENGYLKRDS
                     ASLSNDLVFGLNINMDAEKIEEIIQVIQQLDPPGVAARDLRECLVLQLKRKDRKLESV
                     QLAIQILNECFDEFSKKHYDKIMKRLNISEDQLKDAIDEIIKLNPKPGNSGGDSSKGS
                     LHVFPDFTINNNEGELELVINSKNMPDLRVSRYYQQMLESYVKSKNQDIKTKEAIQFV
                     RQKIDSAKWFIDALRQRQNTLFVTMSAIMEFQREYFLSGDDTKLKPMILKDIADKVGL
                     DISTISRVASSKYVVTPFGTFLLKSFFSESMQTESGEEVSSREIKSILTDVIGAEDKR
                     KPLTDDRLAKILKEKGYNIARRTIAKYREQLDIPVARLRKQI"
     gene            complement(15466..16863)
                     /gene="asnS"
                     /locus_tag="PKY63_08685"
     CDS             complement(15466..16863)
                     /gene="asnS"
                     /locus_tag="PKY63_08685"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPF00728.1"
                     /translation="MKRIKIVDLLKKPAADLLGTTVTVKGWVRTRRDSKNVVFIAVND
                     GSIIHSIQVVADTTKFDEALLKDITTGCSVRIDGKLVESPAQGQPVEIHAEEIEIYGK
                     CPSDSYPLQKKGHTLEFLREIAHLRPRTNTFGAVLRIRHAMAFAIHKYFNDRGFNYLH
                     TPIITGSDAEGAGAMFRVSTLDAENPPRTPEGKIDYTQDFFGKETNLTVSGQLEGELG
                     ALALGEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDITDNMDLAEDFLKSLVSYA
                     LEHCYDDLEFLQKMYDKELIARLKGVVETPFKRLAYTEAVDILMKSGQKFEFPVSWGT
                     DLQAEHERYLVEQHFSCPVILTDYPAEIKAFYMKQNDDGKTVRAMDVLFPRIGEIIGG
                     SQREEDFEKLLNRIHELHIPEKDVWWYLETRKFGSVPHSGFGLGFERLILFVTGMANI
                     RDVIPFPRTPKNAEF"
BASE COUNT         5061 a         3825 c         3417 g         4606 t
ORIGIN      
        1 cagtgagcag tgaatagcga gcggtgagtg gctttcagcg agatcgaggt caagaacaag
       61 gttaaggttg aggttgaggt taaggttaag gttaaggtta aggttaaggt taaggttaag
      121 gttaaggtta agcaccttcg gtgatcagcg agcagtaagc ggcgatcagt gaacctggct
      181 ttgtacaaaa gaagcttttc gcgcttcaga cctcactccg acattccatg ttcaatgttc
      241 agtgttcgat gttccctata atttcctaaa tttgcacaca atttcacacg attacaaaga
      301 tggattacaa tcaccgcgac accgaggccc gctggcagaa atactgggcc gaccacaaga
      361 cttaccgtac ggaaatcgtt ccggacaagc ctaaatatta tgtgctcgac atgtttcctt
      421 acccttcggg agccggactt catgtcggac atccgctcgg atacatcgct tcggatattt
      481 tcgcgcgcta taaaaaactg aaaggtttca acgtgttgca tcccatggga tacgatgcat
      541 tcggacttcc ggccgagcag tatgccatcg aaaccggcca gcatcccgcg attacgaccg
      601 atgtcaacat caaacgctat cgcagccagc tcgataaaat cggtttctcg ttcgattggg
      661 accgcgaagt acgcacttcg gatccggcat attacaaatg gacgcaatgg acattcattc
      721 agttgtttca ttcatggttc gacaagtcac aacagaaggc acgtccaatc aaagatctga
      781 tcacgctttt cgaagacagc ggcaatgaaa atattccggc ggcgcattca tgtgatgtga
      841 ttttcactgg ttcggagtgg aatgtgatga gcgaagaaaa acaatcggac attctgatgg
      901 attatcgact ggcgtattta gccgacacca tggtgaactg gtgtcccgct ctgggaacgg
      961 ttctcgccaa cgatgaagtg agcgaaggtt tcagtgttcg tggcggacat ccggttgaac
     1021 gcaaggaaat gaaacaatgg tcgctacgca ttacggccta tgccgaccgc atgctggaag
     1081 gacttgaacg cattgagtgg cccgaaaaca tcaaagaaat acaacgcaac tggattggcc
     1141 gcagcgaagg tgccatgctg cgtttcctgc tggccgacca tccggacaaa catcttgaaa
     1201 tctttacaac ccgccccgac actgtgtttg gagtgacgtt tatgtcggta gctcccgaac
     1261 acgaatatgt tgaattgctg acaaaacccg aacaaaaagc tgttgttaat gcgtatgtaa
     1321 aacatgcgaa aaaccgcagt gagcgcgagc gcatggccga tgcaaaaacg cccagcggtg
     1381 ttttcacagg ttcatacgcc atcaatccgt tcaacggaaa agctgtccca gtgtgggttg
     1441 cagactatgt gctcagcggt tatggaaccg gcgccgtgat ggctgttccg gcacacgaca
     1501 gccgcgatta cgcatttgcc aagcatttcg gactacccat catcgaagtc atcaaaggcg
     1561 gtaacatcga agaagaagct ttcgaatcga aagaaggcgt ttgtgtgaac agcggattta
     1621 tcgacaacat gattgcccgc gacgccatta aaaaagtaat tgcacatgcc gaagaaaaag
     1681 gatttggcta cggtaaaatc aactaccgtt tgcgcgacgc catcttcagc cgccagcgct
     1741 attggggcga gccgtttcca atttattaca aaaacggaat tccacatacc attgatgagt
     1801 ctgaacttcc gctccagctg ccggaagtag ataaatatct gccgaccgaa accggcgaac
     1861 caccgctggc acgcgcgaaa aactggcata ccaaagcagg acatccttac gaaacatcga
     1921 ccatgcctgg ctttgcagga tcgagcgcct attatttccg ctacatggat ccgcgcaacg
     1981 acgaaaatta tttttcgaaa gatgccgtga attactggaa aagcgtagat ttttatgtcg
     2041 gtggtgctga acatgcaacc ggacatttga tgtatgcacg cttctggaat aaatttctgt
     2101 tcgatctcgg cctgacaccc gaagacgagc cattccgcaa aatgatcaat cagggaatga
     2161 ttcagggacg cagcaatttt gtgtatcgcg taaaagcaga gcgtatggca gaattcgtgc
     2221 tctgggaaat gatccgcaac aaacaactgg gggctgaatt taaaaaagga tacacacaag
     2281 gtgaattttc atttgacttt tacagccccg aaagccacat catcattgag gtgaaaagct
     2341 cacacgacgt tttggaacgt ttgccgaatt gcgcagaatt tgcagaaaaa aacaattgtc
     2401 agctgcttat gttcagcatt gttgatgtga ttttgcatac tgaagatgta ttgaaaaaaa
     2461 taaaaaaagc actatcagga aaagatgttt ctccgttcga aatgattgat gaatgggaag
     2521 aagcacccat ctttgcttca aagaatattt tcggccgcga acaattttca tttcccgtgc
     2581 atgtagatgt gaatattgtc aataatgaca ttcttgatat tgatgcattc cgcgcatgga
     2641 gaccagaata caacaatgca cgctttattc ttgaagaaga taaatatgtg tgtggttacg
     2701 aagtcgaaaa aatgtcgaaa tcgaaataca atgtgcagaa tccggacgat ctcgtagaga
     2761 gatacggcgc cgatacattg cgcatgtacg aaatgtttct cggtccggtt gaacagtcaa
     2821 aaccctggga taccaacgga atcgaaggcg tatcgcgctt tataagaaaa ctctggaaac
     2881 tttttgttgg cgaagaagac aatctgctta tcactgacga cactcccaca gcggctgagc
     2941 tgaaaacgct gcacaaaaca ctgaaaaaaa ccgaagaaga cattgaacgt ttttcactga
     3001 atacttgcgt cagtacattc atgatttgtg tgaacgagct tagtgatcta aaatgccgta
     3061 agcgcgccat tctcgagcca ttgctgattg cgctgtgtcc tttcgcgccc catatttcag
     3121 aagaattgtg gaacagactc ggcaacactg agaccatatc cagagctact ttcccgacat
     3181 gggacgaaaa atatctgatc gaagacaccg taaaatatcc ggtttcgttc aacggcaaaa
     3241 tgcgcttcat tcttgaagta gctgcaggaa ctgcaaagga agaagtcgag aagcttgcgc
     3301 tcagcgcacc cgacagtcag aaatggctcg aaggcaaagc cccgaaaaaa atcattgtgg
     3361 tgcccggaaa gattgtgaat gttgtgatat agcggtgagc gaagtttcaa aacttcgcac
     3421 agcacattaa acttcgctca gcacgataat atcatagaga aatataaaat agtccatgaa
     3481 tctgtatgac tccggaggag tcgtatgtct atagaaaaca ttgattatta aacacgactc
     3541 cagcggagtc gaatgttttt caagctgttt cacacaggta gccgtttaca ttcgacctct
     3601 ctgaggtcgt gactggtata tacatttctc tatagacatg cgatgcctct ggcatcggcc
     3661 atcaatctgc acttaaacac attggcgtag aatatatttc acaatggtat aaaactaggc
     3721 acagcgcttt aaacttcgca caacaactaa ttcataaact gcgacagaaa ataccaataa
     3781 actttgttca tttctttttg gctcacttta cggaaagcag gcttggagcc atcgaaacga
     3841 ctgataatat cttcggtata ttttattatc agttcaacgc cttgcgggtt aatcagcggt
     3901 gtttcatctt ttggcgtatg ataaacaggt ggtaatccgg aagtgaaata aatataaggc
     3961 gttttgtttt tataaaagca gctgtgatct gaagcatcaa caccaccggc aaattttttc
     4021 agtttatact tcccatattc tgtttctggc attaaagaat cccataccat tgcggtgcca
     4081 agcccataaa tatcgagttg gtcagatttc cagcccaggc gaccaatcat atcgaagttg
     4141 atataaaatg caatttgttc aaacggaaag agattgctgg aacaaaaata atttgatccc
     4201 acaaggccct gttcttcgga accccagcaa gccataatgt aattgtattt tgtgagattt
     4261 ccggaactca gatagcgtgc catttccatc acgccggctg ccccgctggc attatcatcg
     4321 gcaccattat ttatatacaa agcactatcc ttataataat aaccaatatg atcgtaatgt
     4381 cctccgataa caatggtata gggagcatta ttttcaatat aacccagaat attgtttgat
     4441 tttacaacaa tagtatcacc tttttcattt gtgattttgc gcaacacaaa ttcctgtctg
     4501 aaatgatcag tcccctgcag tggaatcaaa ccgcattcaa taaactcagc gatgatataa
     4561 tctcctgcac gcatttcata caccgatccc ccatcgcgcc ccatgagaga atcatgtgcc
     4621 agcaatgaaa tatcgtacat aatcctattg gtgcgcaacg aatcatcaaa agaaaaagta
     4681 gtctgcgcaa aagccgcaaa agaacagaat aaaaacagaa aagaatatat tttcataaca
     4741 aaaaattaca gaaaaattta aaaagctaca gtgatgaaaa tggcggtaca accgttattc
     4801 cgggtttata ttcctgagtg agcaatgaaa cgagaaagtt ataatgatca ttgttggcga
     4861 caaaatcgag attgctggta tcagccacaa cgacacggag cgaagattgc tcatgaaaat
     4921 actcgaaata acttttctga atattatcga gatactcgag cgaaatatcc tgttcgtaac
     4981 cacggccacg tctgataata ttttctttca gtttttcggg cggcaaatgc agatataaaa
     5041 caagatcggg gcgtggcaaa ttgctatcga tgatctgaaa aagattttta tacaatctgt
     5101 attcatcgtc ctgcagagtt tttcttgaaa atataagaga tttcgggaaa atataatctg
     5161 aaatcagata atcataaaaa atatcagcat taagcacatt gtctttaaac tgcttgaatc
     5221 tggcagccag aaacgacaat tcgagcggaa aagcatagcg ctccggattt ttataaaatt
     5281 ttggaagaaa atcattttcc tcaaattgct caacaatcaa cttaccattg aaatcggccg
     5341 ccaaccgcgt ggacagcgat gttttaccgg caccgataca accctcaaca gtaataaaat
     5401 gatatggaaa tttcatgaca acgcgttagc gtttcaaagt tacagtaagt tttgattcat
     5461 cgggacattc accaagtaaa accttgacag ttttttttaa cactgggtga ataaagtccg
     5521 gacacagctc tgcaagcggt tccaggacga agcgtcgtaa atgcatttta ggatgaggaa
     5581 tcgttaactc ctccgtgttt acaaccaaat cgtcgaaaaa aacaatgtca agatcgatgg
     5641 tccggtcggt atattcagaa aaaatgctgg gtttcaggcg tccaagttta acctcgatgg
     5701 cttcaagttt gagcaataat tgatgtggac tgccttcggc cttcatttca agacaaagat
     5761 tcaaaaactg tttttcggat ttaaaaccaa tcggttctcc ctcatagatg ttgcttacac
     5821 gaacgacttc gccaatatag gaacgaatca tatcaatggc ctgctgcagc agccattcac
     5881 gatttccttc attcgacccc aaagacaaaa atatattgtg gcgattcatg attacaaata
     5941 tagaaattac ttttctatct gcaagaaaaa caaagttaat ttgttaaaaa aggaagttct
     6001 ttacataaca tagcaaaata cgcaagtctt tatttttgag ctttccattc gagcagaaat
     6061 tcggaaagaa aagcttcgag catggcatga cgtccttggg ccagtcgttt tccggcatca
     6121 gtttccatac gatctttcaa caacagcagc ttttcataaa agtgatttat cgtatgggat
     6181 ttactgtttt tataatcact gaagttttcg tgcaatgcag gtttctggcc atcatcaaac
     6241 attggcctgc ctttaagggc tccatacgca aatgcacgtg caattccaat acttcccatg
     6301 gcatcgagcc ggtctgcgtc gcgcacgcag caaccttctg cagacatcgg agcgtcatca
     6361 accccattcc ccttaaatga aactttctca acaatatcaa taatctgatg agtctgatca
     6421 tcagtaacac ctgtttcgtt caacaatgca aaaattgcat ttctgtgatt ttcgtttccg
     6481 tctgcggtaa gtttatgatc tccaacatcg tgcaacaaag ccgccagaga tacaataaaa
     6541 acatcacaac cttcttcttc cgcaatcctt gttgccatat gataaacacg caaaacatga
     6601 tcgaagccat gtccgcttcc gtccatcggg aacaacgcac gaacactgga caacaccctt
     6661 tcaataatct gccggttcaa ataatcatca cgcattccaa aagtatctgt atttacggat
     6721 tgttttttcg ggtttcattt ttccgctgta aatgcggtat tcaacaatca cttctttttt
     6781 aattcttcct tttgaagtaa agaaattttt tgtttcccgg accacataac cgtcagccca
     6841 atgctgttcg agcacaggcc gggagccatt gcgaccatat ttccatttca cggtaagcgt
     6901 tgtatcacca tgagaaataa catcctgacg gagtttaaaa ccaaacttat cgtaagtata
     6961 acgaatatct gaaatggtgt cttcgccatt cagtcctacc accctcaaca tttttccatc
     7021 cgtattgtaa aaatataatt ccgacagaat cattatctga ttgctatcag aaatattttc
     7081 cagccgcttt ttcaccaacc cttccggggt gtattcataa acagttacaa ttttttttcg
     7141 ttttccgtga taagcttctt cttcaacaag ccatcctttt tcattgtatt tataaaacgt
     7201 ttcacccgac acaatgccct tttgtgattc ccgttttttc tcaagctttc ctttatcgtt
     7261 gtatgaatat gagtaatctg ttacatccga ctcattgata taatgtcctg aacgaataag
     7321 tttaccctta tcataaatca taaattcacg aaaccacaat aaaggatcgt tactcagaca
     7381 cgtttccttt gcaaggagat ttccatgaat accgtatgat ctgcgttcaa ccagattttt
     7441 cttttcaaaa acacgaacag taaaaaccgt atcctgtgca ttaccggcaa tagcaattgc
     7501 ggcaataaaa accagaaatg tgattgtttt catcatttat tgatttttgt ttttttcttt
     7561 ctttcccaaa gctccctgaa cttatcaaaa tcctttctgt aaaaaatggc aaccccttgt
     7621 gtgtaaggag cattgttagc aaccacatcc accgtattgg aacgattaaa ggccttgatc
     7681 cggattttac cgtcttccga aattttatat tcaatactaa catcaccagc tatgtttgat
     7741 gcttgctgat tatcgggcga tccaagctga ttcccgccaa tttcaagatt ggtttccaaa
     7801 acaatgcgat cattcagaat ctgcgtactg agcgcgattt cgacctgcga cgaagtcacc
     7861 tggtcgccct gattgtaact gaaaccaaca tcgaaatcat tgctgatctg cgacaaccag
     7921 ttgccaaact gatttgaaat aagctcaatg gatgtattgc cgagtccctg actgataggg
     7981 ctgttgaacg tgttgcgctc tgtcggaata aatgaattaa ggataagcaa tgcaaacacc
     8041 tgacgattca tttcctctgc attaccaatc acagttctga caagattttt tgtattctca
     8101 tccgattccg gcaaatcgag atcgaaggaa atatcggggc tcagcaagtc acctgtcagg
     8161 tgcatgagac aacttacagg aactttgttt ttatacagct cagattcatc ggcaacagaa
     8221 accagctcat agagcgaagt tttaatggaa tatcgggcat acagatccat ttttcctgca
     8281 tacggatcgc cgttccacgt aatcacaccg ccccgatcaa ccaaaaattt cttattgata
     8341 acattttcca gagtaaataa ataatcacct ttttcaatct caagatttcc aaacatcaga
     8401 aattccccgg aacggtcaat agtcatgcgc aaacggcctt caccacggcc tttcatcacg
     8461 tcaccgattt ttggatcgaa cacaatctgc acctctgcat cgggagttac atcaatatca
     8521 aacatcagac taaccccaga atcacgaatc ttgacatctt tctgcactac gcttgaatca
     8581 tttttgttgg aaacaaaagt gataaaatca ttttcataaa cctcggtcga tgatgtaatc
     8641 ggaatataaa gcacggagcc aggttcagat tttgccacca cttcaatggc aatctgattg
     8701 aaagggccgt gaattttcac aacacccgaa gcataggccg ttccataaaa ataatcatta
     8761 tccttggccg ttgtatttaa gaactgcatt ttttcaggcc ggagattaat gtcgagatag
     8821 aaatttttaa aataatcatg cgtggcaaga atattacctg tggccttgtt tcctttagta
     8881 tcgaacacag tgatgtcgcg caacgcaaat tcactgttat ttattttcaa tgtatcggca
     8941 aacgagtaaa atatattcag ataatcgacc agcacgctgg ttctgcggaa aacgacttcg
     9001 ccacttacat ctggcttatc aatatttcct ttaaccagaa caagaccatc gcaggatccc
     9061 tctaccctgc tgcaaaagga tttcagatat ggttgcacaa gttttaaatt aaaccgatcg
     9121 agtttaacaa caaggtcgag catgttttct ttgtcacgcg ggttgaaaaa acccttaatt
     9181 tcaagtggga cattttgtcc gacatttccg gtataaacaa tggaagcttc gctgtacaca
     9241 gcttttcgca agggatccca cgcactttta agtaaggcat cgccgacaaa attcttgttg
     9301 acactaagct tctgcaccgt aacatcagct ctgtaatcag gcacatcata taagttgctc
     9361 aaaagcacat aaccatttac tttcccatcc aaatcaatat tcgaactatg ggtcagatcg
     9421 tcaaaatcat caagcaatac attctcaaac gagacctgaa caacatcgta ggggtctttg
     9481 gaaatgcggc catctatcat caatttctgt gatttggaat acagtgcaaa ttcatgaata
     9541 cgaaggtagc ccgtatctat ttcaattttt ccattctcag caatgtgata cagcgagtca
     9601 ttcagtgtca ggtcgctttc gtcaaactga ataagcaccg gcattccttt acggaacaaa
     9661 acattaccgc tcagatcgcc ggaatatgct tttgtgctgt tatcatttct ccagttgatg
     9721 gaaaaatcag atttatcatt caccgtaact gtattggcca ccagattacc aagttcaaga
     9781 ctatcattat atgtcaggtt atcggcctga accgttacat aaatattttt cccaaaagtc
     9841 tgagcaatca caaccggatt aaccgagctg atggcgcctg atttgagata attggaagca
     9901 atctgggtgt ttaggaaatt ctcttccaga ttgaaatttc catgcaaact ggtgttttcg
     9961 cttatttgaa ttcctttcgc aaaaatatca aggacaggtt gaacgtccct gaaagtaaat
    10021 tcaaattcaa aggcctgatt gctgagtttt tttgcactac tgtcaggcac aatataagcc
    10081 gggatataat ttgaaagata cttgttgaat gcatcatcga tttcggaaaa aacaaactga
    10141 cctttaatat taccattaac cagatcactc ctgagttcga taactttatt aacagaatcg
    10201 gttttctcca gtttcaacga aagtgaattc atatcaattt cattcgggcc ctcataatag
    10261 ctgaaatcat tgagtacaag acggcccaac atattatccg gatgatttcc tatcacatta
    10321 atatcgataa aaccagaaaa accggcaaga ctatcattgc gggcaagatg aagggccgac
    10381 aaatcaatgt tttcaacctg agcaataaaa tcaaataccg gaattttttc tgaaaaatca
    10441 atcattccat caaaatcaag agacaggttt ggatcactgg caaggagctg tccttcaaaa
    10501 attccaggtt taacagaccc atttacactg atatccgaat acaaataatt gttatattca
    10561 agagaaagaa tttctgcacc gccttcaata aaataacggg ttgcactcgt tcccgatccc
    10621 gaaaaatcca ccgaagcagt cagtgcaccc actggcacag attcacctgc aaatgtcttc
    10681 agatcgaaat tttcgacaac aatattgccg ctataagaag gattaggaaa ttcgccctgt
    10741 gattgaatat cagctttaac aaatccggaa ttggttttgg ccacaatatt tgcactaaag
    10801 tcattcaact ttccatcaat ctgagcagat aaattggctt gattgacaga tcgagctaca
    10861 gaagccagtt tcaatgtgtt gcctcccgga aggacaaatg catcaatatc ggccaatttt
    10921 aaatcagcat taaccagatc gaaatgaacc tccgcattat taatatcagg cagacctatc
    10981 aactttagat tgaaatacat gtgagagctt ttaccaaagc ccagtttcat ttgcccggtt
    11041 tcgagatcgg caatggtacc accagcatga ccattaagat ttacaacgct gcgcatgcca
    11101 ttcatggccg gaacaaagcg ggcaatatca tcaaagccaa ccctcgagtt ccggaaatca
    11161 gcatccagtg acactttgtt tacaaaatcg ctgaacgctg aaatggaatc atatcggaaa
    11221 accaggtcca gatcgagatt gctgctgttt gtcagaaaac gtaatttctg cgccgtaata
    11281 ctgttcttcc gcatggaaac ctgagaagac aaatgccaca aggtaaatcc tgaacgatcc
    11341 tgcatggcca gatgcttcag gtcaatgctc gtcaatgtat cgcattgata cagattctga
    11401 ccgatcaggt taagatttgt cagatacaga ttattgaaat caatattgtt ttttgcagaa
    11461 gtgtctttgc gcatatcctg ccagaagaaa ctgcagttgc ggagttccag catgccaaca
    11521 ctgactggcg gcgattcggt ttttttcgtg cttgtatctt tttcctcacc ggcaaaataa
    11581 tccagtataa actgcaggtt cgttttatct tcatcgggat aacgcaccat gcggattctg
    11641 gcttcattca gcaaaagctt ttcaaactgc agttttgaaa aaccggatgc aaggttttcc
    11701 ggggcaacca aaagttcttt cgcggcaagc aacgggtgcc cgtgctggtc ttcgatatac
    11761 agattgcata aggtaatgtc aaccggaaaa gtaatatcaa ccgttccaat ttcaactttt
    11821 gtatgcagct cttctgacaa gtattcagaa agacggttag caatatattg ctgaatgaga
    11881 ggtgtgcgca gcaaagcaaa aacagcagcc ggcaaacaga agaccaccac atagatgatc
    11941 aacagaatac gtccggtttt tttgctaatt ttgctcattc aaaaatgcat acttacatac
    12001 ttggcataga atcatcgtgc gacgacactt cggcggcagt gctgcagaac aactttctgc
    12061 tgagtaacga gattgcatcg caaaaagtac atgaacgctt cgggggcgtt gtacccgagc
    12121 ttgcatcgcg tgcacaccag caaaacatta ttcctgtagt cgaagccgcc ataagtcatg
    12181 caaatataga caaaaaacag ctttctgcag tcgcttttac aagaggtccg ggtttgcccg
    12241 gatcgctgat ggtaggcgtg agttttgcca aggcatttgc ctgggcgctc ggaatcccgc
    12301 ttattgacgt cgatcacatg catgcacata tcatggccaa cttcctgcgg gtaccaggcg
    12361 aaacccgctc tatgcctgag tttccatttc tttgtctgat tgtttcaggt ggccataccc
    12421 agattgccat tgtacgaaat ccgcttgaga gagaaattat cggcaatacc atcgacgacg
    12481 ctgcaggaga gactttcgac aaggcagcca aaatcatgag tcttccctat cccgggggtc
    12541 cggtgattga caaactggct caatcgggca atccatccgc ttttcggttt gcaaagccac
    12601 gcatcgcgga tctgaatttc agcttttcgg gattaaaaac atccatcctg tattttttgc
    12661 gcgatgagct gaaaaaagac gaacagttta ttgaaaaaaa cacagccgat ctgtgcgcat
    12721 cgattcagaa aaccatcatt gatattctgg ttgacaaaac agaaaaagca gtgaatggaa
    12781 ccggcatcag aacgctggcg ctggccggtg gcgtatcggc caactcggta ctcagagcgg
    12841 ccatgcggca actggcggac aaacacaaac tggatcttca tattccgaaa ttatcgtaca
    12901 ccaccgacaa tgccgccatg atagccatgg ccggctattt cagatatcag aagggattgt
    12961 tcgcaggcct cgatgcagcg ccgtatgcga cgggcgtgaa ggtgtaatat tcattctgtt
    13021 ccggtagcca ttgaaagtcg ctcctgcagt gcggtataaa atgagatcct gggtcaacgt
    13081 acaacgttgc gcggcgcgca agctttgctt tttcgaagct ggttctggct tgcccgggaa
    13141 cttcaaactt cttctgttga ttctcagata ttcacagagc aagtttgttt tcaaaacaga
    13201 ctgctcaatt atatgaaaat ataagaatca aaataaattt acagaatcac caacaccacc
    13261 accggcagca gcccgagccc gaagccgaga tagatttcac caggcgtgtg tgcattcagc
    13321 ttcaggcgcg accaagcaac aacacccgtt aaaacaacgg ccagggcaag atacagaaca
    13381 ctgaaacact gccatgaaaa aatataccag gcaatggccg caactatcac actccaacca
    13441 aaaatatgca gactgaattt tttgaaaaaa gaaacaataa gtccaaaggc agaagcagaa
    13501 aaaatcagca gcagaaaatc ggtgtagtat gcaggaagtt cgtaattgga caatatccac
    13561 cagcaaaacc atgcagaaat tccaatgaca gtgactgcaa aaatgcgttc ctgttttatt
    13621 tccatttcaa gcgactcaat aacgcccaga tagcgcgatg cgacaaaaat cagagccgga
    13681 atgaccagcg taaagatcaa aacaaccaga ataattatcc tgacagcttc aggacgcagc
    13741 atattgttga ggtaataatc tccgtttagc gccaacacag caaataatgc cggaagtatg
    13801 attggatgta atagccagga gagaagtctg gggaggttat tcattttagc tgtaatatgg
    13861 aaattaaaaa atgtgcgaac gcacattttt taattaaatc tgtttacgta aacgtgcaac
    13921 cggaatatca agctgctcgc gatatttggc aattgttctc cgtgcgatgt tgtatccttt
    13981 ttctttcagg atttttgcaa gacgatcatc ggtcagtggt tttcgcttat cttcggcgcc
    14041 gatgacatcg gtgagaatac ttttaatttc gcgggacgaa acttcttcac ctgattcagt
    14101 ctgcattgac tcactgaaaa agcttttcag aaggaatgta ccaaaaggtg ttaccacata
    14161 cttactgctg gccacgcgcg agatggttga aatatcaaga ccaaccttgt cagcaatatc
    14221 tttcaggatc atgggtttca actttgtgtc atcaccactg agaaaatatt ctctctgaaa
    14281 ttccataatg gcgctcattg ttacaaagag tgtattctgg cgctggcgca atgcatcgat
    14341 gaaccatttg gcagaatcaa ttttctgacg aacaaattga atagcttctt tggttttaat
    14401 atcctgattt ttcgatttaa cgtacgattc aagcatttgc tgataataac gactaacgcg
    14461 aagatcaggc atattcttcg aattaatgac caactccagt tctccctcat tattatttat
    14521 cgtaaaatca ggaaaaacat gcagactacc tttgcttgaa tccccgcccg aattcccggg
    14581 tttaggattc agcttaatga tttcatcaat agcatctttc aactgatcct cacttatatt
    14641 gaggcgcttc atgattttgt cgtaatgttt tttcgaaaac tcatcaaaac attcattcag
    14701 aatctgaata gccaactgaa ccgattcaag tttgcgatct tttcttttta gctgcaaaac
    14761 cagacattcg cgcagatcac gtgcagcaac acctggtgga tcaagctgct gaataacctg
    14821 aataatttcc tcgatttttt cagcatccat attaatattc agaccgaaaa caagatcgtt
    14881 ggaaagtgag gccgaatcac gcttgagata accattctcg tcaatatttc caataatata
    14941 ttcgccaaca cgatactgca attcattcaa atcgcgcatc tgaagctgtt cgtaaagttg
    15001 atcataaaaa cttgactgcg aaacaaaagg gacttcgcgc acctcatcat cggcactcgt
    15061 attttttgca gccaacttgt attcaggaat atcatcctca tccatataat catcgagact
    15121 gaaatcattc tcaacatttt cctcaatatc agaatcttct ttttcatctc tttcctgctc
    15181 ttcgtcctga aggtcctgat tcagatcatc ttcattatct tccgatgtat cctcaagtgc
    15241 cggattttcc tcaatctcct gtttgatgcg ttgttcaagc gcaatggccg gaacctgcag
    15301 cagtttcatg agctgaatct gctgcggaga gagcttttgc tgaagtttct gttcgagcct
    15361 ttggtgaagc atatcaaata atttatgtct gaaaatacga aatgaaaacg gggcttgtca
    15421 agtcttatga gaattattct gcagtttttt tcaacaaatt caatattaaa actctgcatt
    15481 cttgggtgtg cgcgggaaag gaatgacatc acgaatgttt gccatccctg tcacaaacaa
    15541 aatcaaacgc tcaaaaccaa gaccaaatcc actgtgtgga acggaaccaa atttacgtgt
    15601 ttcgagatac caccatacat ctttctccgg aatatgaagc tcgtgaatac ggttcagcaa
    15661 tttttcaaaa tcctcttcac gctgacttcc acctataatt tcgccaatgc gcgggaacaa
    15721 aacatccatg gcacgaacgg tctttccgtc atcattctgc ttcatgtaaa aagctttgat
    15781 ttcggccggg taatcggtca gaatcaccgg acaactgaaa tgctgctcaa ccagatagcg
    15841 ctcgtgttcg gcctgcagat cggttcccca tgaaaccgga aattcgaatt tctgaccgct
    15901 cttcatcaga atgtcaacgg cttcggtgta tgcaagccgc ttgaacggcg tttcaacaac
    15961 acctttcaga cgggcaatca attccttgtc atacattttc tgcaggaatt ccagatcatc
    16021 ataacaatgc tcaagcgcat aactgaccag cgatttgaga aaatcctcgg ccagatccat
    16081 gttgtcggtg atatcgtaga aagccatttc cggctcaatc atccagaact cagcgaggtg
    16141 gcgcggtgta ttgctgtttt cggcacggaa tgtaggtcca aaggtgtaaa tctcacccaa
    16201 cgccagtgca ccgagctcgc cttcgagctg tccggaaacc gtcagattgg tttcttttcc
    16261 gaaaaagtcc tgtgtgtaat cgattttccc ttcaggtgta cgcggcggat tttcagcatc
    16321 gagcgtgctt acgcgaaaca tggcgccggc accttcggca tcgctgccgg tgatgattgg
    16381 cgtatgcaga taattgaatc cacggtcgtt gaaatattta tgaatggcaa aagccatggc
    16441 atggcgaata cgcagcaccg caccaaatgt gtttgtacgc ggcctcagat gcgcgatttc
    16501 gcgaagaaat tcgagcgtgt gtcctttttt ctgcagcgga taggagtcgg aaggacattt
    16561 tccgtaaatc tcaatttctt cggcatggat ttcaaccggc tgtccctgag cgggtgattc
    16621 gaccagtttt ccatcaatgc gaacgctgca tcctgtggta atatctttca gcaaagcttc
    16681 atcaaacttt gtggtgtcgg ccaccacctg aatggaatgt atgatgctgc cgtcgttcac
    16741 ggcaataaaa accacattct tgctgtcgcg gcgggtgcgc acccagcctt taacggttac
    16801 ggttgtaccc agcagatcag ctgccggttt tttcaataag tcaacaatct tgattcgttt
    16861 catcggaaaa gtatttcggg acaaatttcg gaaaaaattc gttgtaaaa
//
LOCUS       DAOPKE010000062        15553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_3987_length_15553_cov_13.029230, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000062 DAOPKE010000000
VERSION     DAOPKE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_15553_cov_13.029230"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1933)
                     /locus_tag="PKY63_08690"
     CDS             complement(248..1933)
                     /locus_tag="PKY63_08690"
                     /inference="COORDINATES: protein motif:HMM:NF017472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00729.1"
                     /translation="MKRLFAFLVVMSAVTLLFAQVPDKMSYQAVIRNSTDILVTNTQI
                     GMEINIRQGSVSGSVVYTETQTPTTNDNGLVSIEFGGGPGFNTIDWSVGPYFLETKTD
                     LAGGTNYTITGTSQLLSVPYALHAKSADTVVGGITETDPVFGASPSGSIAGSDILNWN
                     NAYAWGDHSAAGYLTSYSETDPLWMAASSGYYTKTQMQTSGQSLLHWDNVTNKPASLA
                     DYGAELDPSWAGTNDSTGNIFRTGKVSIGSSDTAALLYTYGDGTGGGNVLFVGSFKGS
                     SGGNTPASGSGTRMMWYPDKAAFRVGEVNGDIWNKDSIGNFSFAAGYTTKAKGSGSIA
                     MGYFSVAYGDFSFATGNNSSATGYNSSAIGYSVTASGDYSTALGSLTMSSGDCSTAMG
                     AMSMSSGDYSMTMGYVTSAYGNYSTALGYYSTASGNASTAMGYYSTASGSYSTAMGYS
                     ITALSANEHVFGRYNTSYSPLNVTGWDAADRLFVIGNGAGPSNLSDAMVVLKNGHIGI
                     GISTPARTLHVNSVMRLQPTAIAPASPAEGDMYMNATTHKLMVYDGSIWQACW"
     gene            complement(1936..2355)
                     /locus_tag="PKY63_08695"
     CDS             complement(1936..2355)
                     /locus_tag="PKY63_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00730.1"
                     /translation="MHESISASGGNAIGSAGSVSYTVGQIVYSTNSSTSGSVAQGIQQ
                     PYEISEVVDGYEQLSEHLYCSVYPNPTTDFLVLEIDNAAEVIYQLYDATGKFVASRIV
                     TDATTMIDMNGLVSGTYFLKILKDNQMVKTYKIIKNK"
     gene            3124..3246
                     /locus_tag="PKY63_08700"
     CDS             3124..3246
                     /locus_tag="PKY63_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00731.1"
                     /translation="MLFLFRREAVYHSMHMHDPRTISKEETWWRMNNVEKFKRR"
     gene            4116..4505
                     /locus_tag="PKY63_08705"
     CDS             4116..4505
                     /locus_tag="PKY63_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00732.1"
                     /translation="MKKWLFVLVLMISFNGLKAEGGDTIYAGVNLSEEFLLYMPNAFS
                     PNGDGHNDYFSPVGAGITLDYFEMRIYNRRGKLVYLTTDINQPWDGDITGKRYDETST
                     EVFVYHITVRTYKGIERDYYGHVVRLP"
     gene            4509..5429
                     /locus_tag="PKY63_08710"
     CDS             4509..5429
                     /locus_tag="PKY63_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPF00733.1"
                     /translation="MNRFKFLKEFLNLSKTEKAGIWLLLTILISIILLSVFLHSTKTE
                     LADSDFSDFEKQIQTWESSISSDNQQYSNVYNDLDAPAYSAAKSKLNPFPFNPNELDA
                     EGWKRLGLTDKQTKSILNYRQKGGEFRIKSDFSKMYTISKEEFAVLEPFIELPEKPQQ
                     RPDYSASTYEKKEVIRVEINSADTAALKKVRGIGPAFALRIVGYRSRLHGYISIDQLK
                     EVKGIDSLKFAEISPYMFVNPYLVRKININTATFDELKSHPYIGYNIALSLINYRKQH
                     GDYKVLSDIKKSALVTEDIYKKISPYLTAQ"
     gene            complement(5614..5979)
                     /locus_tag="PKY63_08715"
     CDS             complement(5614..5979)
                     /locus_tag="PKY63_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPF00734.1"
                     /translation="MKLTTKRKAKKTTKTPEIAEEQFDILVTEQGEYKTRFSKKYLNR
                     KSYAPLNIKHVISAIPGTIIKLFSAVNDEVAEGDVLLELEAMKMHNKILAPMSGKIKA
                     IHVKEGERVPKNTLMVEFE"
     gene            complement(5993..7537)
                     /locus_tag="PKY63_08720"
     CDS             complement(5993..7537)
                     /locus_tag="PKY63_08720"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPF00735.1"
                     /translation="MSIRSKSMDLRKRVLDARQGGGTKAIAKQVAMGKLTARERILAL
                     LDPDSFHEYDLFVEHAGRDFDMDKKHLPGDGVITGTGTVNGYPICIYAQDFTVAGGSL
                     GYMHAMKITKIMDHAMKMQVPLIGINDSGGARIQEGVNSLAGYGEIFYRNTLASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEEISMEDLGGARV
                     HAEITGNAHFFARNEFECFAQIKALLSFIPWNNTKKADRFEPMKPNKNYKITSILPTD
                     PKTPYDVREVIRALADDSDFFEIMELFAPNIVIGFARIEGDTVGFVANQPLVLAGVLD
                     VDSSDKAARFVRYCDAFNIPIITLVDLPGYLPGVDQEFAGVIRHGAKLLYAFSEAIVP
                     KITIILRKAYGGGYIAMSSRHLKADFVFAWPTSEIAVMGPEGAANIIFRSEIQAAENP
                     EEMRLKKVQEYKEKFANPYVAAAYGYVDAVINPAETRSFIAHALEVSVSKMVTRPTKK
                     HGIPPF"
     gene            7826..8290
                     /locus_tag="PKY63_08725"
     CDS             7826..8290
                     /locus_tag="PKY63_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00736.1"
                     /translation="MKHFYLFLFLMGGLIAETAEAQHFSFTPPENFVADTLNSGSTNW
                     FNKEAGSVIQITETTGTAFNTFKENFNEEQLTNNNLVIKEKKPAGPALLYICSYRTMA
                     ADGSRDVEFTRVIFFTGNANAVVMAVATIPAMAEPVLLEPIIQSFVKDLIIR"
     gene            8318..11089
                     /locus_tag="PKY63_08730"
     CDS             8318..11089
                     /locus_tag="PKY63_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00737.1"
                     /translation="MKRILPVLLFCASLFAFTHSKAQTFTVNHQIPWSTDYQNMWGPN
                     GSPFSLNFDYSLFHFMYDSTTTFGQIENILGGDFGAEFDLDMYLEMGSTFSIHGFTTG
                     WVDVDYPVDIELTFPDPGLPVPGQYMTVNSDYDVLPGWDLSSHFPQAGVVTLDLDFGM
                     SMDLSGQVCFIGCTPVNIANFDIPLDSIIIFELNSITGEVTYPCINGFLPAICHDTIL
                     PLIITNIGGIGLDLTADIPYIQTIDSLGSDKCLYAYGDDWYLELSLDIIEFLSFIANI
                     IPPPAGPAIASVLSMLNGTYDLGYGISIDYSLLSAFLTLNNTLQQDLTFCPDVWTELT
                     YPIALDYFITDPIAGDTVGTGTNDSISFMAGHDLHVKWPCFGYTEFDPGIRHFLTNDF
                     TNHTWDSMAFDFSIQAFEFTINFPSFPVAPASDLPELCVAIPQNDSAATRALCVDPVQ
                     TPEVYLDMSKLSFHIGPLVDYTIPLGYIPLTWYLNTWELDGFHDTIFPSVHLESGPFF
                     QMTILGDTTICYGDSTGTLIATGINGASPYSFQWSTGENDSHSWPTDSIFVPSGLYSV
                     TVTDGGGCTLSDDVIVVDNPQIFVSLSKTDVNCFGDSTGTILSTVSGGTPGVAPGYNW
                     LWMPGNASSQNITNIPAGFYTLVVTDSLGCTASDTITVNELHPHPPVDVMADPSVGCQ
                     PLLVQFDEINPSTGNSYNWDFGDNSTFSIAEDPLHEYDTAGTFSVTLTVTNEWDCATT
                     ETYNDLIMVYPKPAASFYATPDLVYTSEDPSMTIDFTNTSSGEIAWLWNFADSTSANN
                     TSTFENPSHSFSDEGLFNVMLIVTSDLGCMDTAWVSVEVIDDVLVFPNVITPNNDGFN
                     DVFEIVNVEKFPECKLTVFNRWGNIVYMASPYRNDWDGENSADGTYFYVFIYGKDSKE
                     YRGSLTIIR"
     gene            11126..11788
                     /locus_tag="PKY63_08735"
     CDS             11126..11788
                     /locus_tag="PKY63_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF00738.1"
                     /translation="MKNLFAFFFLALFSVTLLAQEEDLELSNRFGKADRLMIDLYNDT
                     WLGAPDSIDFKGYNPGVAINLMKDFPMGATNFSFAIGVGIGTHNMRYNATIFNDPVTF
                     ENYFVNRLDTPSQYKMVLSFIDIPAELRFRTKRDNVFRIAIGGKIGYNINNHIKHVDD
                     DFKIKTYNTEYINKLRYGVTARIGYKMFNLYVYYGLSTLFEEKHGPEMAPLSIGISLI
                     PF"
     gene            complement(11851..12954)
                     /locus_tag="PKY63_08740"
     CDS             complement(11851..12954)
                     /locus_tag="PKY63_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00739.1"
                     /translation="MKKFLIFSILLLSVNFIFAQGAWKLVGYKFVDGTSSSEKPLMGT
                     ADYMIDAIKFIGGKGSIQITKNRNDKSTGKLLAGVTYKVEWTDPADIVVVGEKMKIQY
                     TLKTISTFASWTPEAQSVYFEQGYPGNFLRNPAGENYFKKDFSAAIVSEKPIVKGTKN
                     NEQKKFIVNLGCGFKAEYVYEWLPDYTAGTSMNTTSQAVGGNQPGWYFVKYEFIDGSS
                     SSVKPLAGTVDYMVDASKFTGSKGNIQITKNRNDQSSGKLLAGTTYNVTWTDPQAYLA
                     AGGSFKMKYTLKTISTFASWTPEAQSVFFEQGYPGNFLRNPAGENYFKKDFSADIVSE
                     KPIVKGTKDKEMKKLTVNLGCGFKAVYYYEWRN"
     gene            complement(13108..14325)
                     /locus_tag="PKY63_08745"
     CDS             complement(13108..14325)
                     /locus_tag="PKY63_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPF00740.1"
                     /translation="MIDTGSTGFMLLATSLVMLMTPGLAFFYGGLATKRNILGIMIQS
                     FVSLGWTTVLWVAFGYSLCFSGGAGGIFGNFDKAFLAGVGMDSMYTNGKIPEFVFIAY
                     QMMFAIITPALITGAFANRVTFKSYMIFLTVWQILVYYPFVHMIWGGGMLAEWGVLDF
                     AGGIVVHATAGFAALASVFYVGARVDKNSTPNSIPLVAIGSGLLWFGWYGFNAGSEVA
                     VDNITSLAFLNTDIAASFATVAWVVIEWIHVGRPKFIGLLTGSIAGLATITPCAGFVP
                     LWASPIIGVAAGAVCYLAVQLKNKMKWDDALDVWGVHGIGGVIGTILLGVFASSAVNA
                     AGADGLYFGGGDFFFKEIVAVAAAAAYAFIFTYIMLILINMVVKVRVSEDDERKGLDV
                     SIHGETAYDEGAL"
     gene            complement(14347..15111)
                     /locus_tag="PKY63_08750"
     CDS             complement(14347..15111)
                     /locus_tag="PKY63_08750"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00741.1"
                     /translation="MKQKNRLAILLIFAALMPLSISAQDSSKVKFNSGADIMSRYVWR
                     GTDFGASPSIQPAISLTGFNFIFGVWGAFATNNFSQAQETDLFLTYTLKDKVDFTITD
                     YFFPNETPGAFNNYFEYNDTLTGHIFEGTIKWKGTEDLPLSLLVAANVYGADTRHADG
                     KLFYSTYVELCYSGTCKSADYDLFAGFTPNKPTDNDKLSGITRGFYGNTMGFVNLGIK
                     VKKEVKITNDFTLPLSTSFVVNPMAENVFLVFGFTL"
BASE COUNT         4467 a         3618 c         3096 g         4372 t
ORIGIN      
        1 aagttaatca cttttttcaa cttaacatgt ggtccgaaat ctggggagta ttatatagaa
       61 atcgcctgaa aattaccaga atttactcgg attcacctga aaattactcg atttttacta
      121 aaaaatgatc cggcggagga aaatgccggg aaaaataatg ttttatatcg catttgtcag
      181 gtttgagctg gcaagattga tataacaaaa actgataaaa aatctgataa cccatattca
      241 ttccggatta ccagcacgcc tgccagatag atccatcgta caccatgagt ttatgtgttg
      301 ttgcattcat gtacatatcg ccttcagcgg ggctggctgg agctattgct gtcggctgaa
      361 gtctcatcac agaatttaca tgcagtgtcc gtgccggagt gctgattcca attccaatat
      421 ggccattttt taaaacaacc atggcatcgc tgagatttga tggacctgcg ccgtttccaa
      481 ttacgaacaa acggtcggca gcatcccatc cggttacatt gagcggtgaa tagcttgtat
      541 tgtatcttcc aaacacatgt tcattggcgg acagagccgt tattgaatac cccattgccg
      601 tggagtaact tccagacgcg gtagaataat atcccattgc cgtggatgca tttccagacg
      661 cggtagaata ataccccaat gccgtggagt aattaccgta tgcacttgta acatagccca
      721 ttgtcatgga ataatcaccg gacgacattg acatagcacc cattgcagtt gaacaatcac
      781 cggacgacat tgttaaacta cccagtgcag tggagtaatc tccggaggcg gttacagaat
      841 atccaattgc agaagaatta taaccagtcg cagaggaatt attccctgtt gcaaaagaaa
      901 aatcaccgta tgctactgaa aaatatccca tagctataga gccagatccc tttgcttttg
      961 tggtgtaacc agctgcaaag gaaaaatttc caatgctgtc tttattccag atatcaccat
     1021 ttacttcgcc aactctgaat gcagctttgt cgggatacca catcatgcgc gtaccagagc
     1081 cgcttgcagg cgtattgcca cctgaagaac ccttgaatga accaacaaat aacacattgc
     1141 caccacccgt accatcgcca taggtataaa ggagagccgc agtatctgaa ctcccgatac
     1201 ttacttttcc tgttctgaaa atattacccg ttgaatcatt cgttccagcc caggatggat
     1261 cgagctctgc accataatcc gccagcgatg caggtttgtt tgttacatta tcccagtgca
     1321 gtagcgactg accgcttgtt tgcatctgcg tttttgtgta ataaccagat gatgctgcca
     1381 tccacaatgg atcggtttcg gagtaacttg tcaggtatcc agctgcagaa tgatcgcccc
     1441 aagcataagc attattccaa ttgagaatat cactgcctgc gatgctgcca gaaggcgagg
     1501 cgccgaatac cggatccgtt tctgtgattc ccccaacaac agtgtcagct gattttgcat
     1561 gcaatgcata cggaacgctc agcaattggc tggtgccggt aatggtgtag tttgtaccac
     1621 ctgccagatc tgtttttgtt tcaagaaaat atggacccac cgaccagtca atggtattaa
     1681 aacctgggcc gccaccaaat tcaatgctta ccagaccatt gtcattggtt gttggagttt
     1741 gggtttcggt atataccaca cttcctgata cagaaccctg acggatattt atctccattc
     1801 caatttgcgt attcgtaacc aaaatatcag tgctgttacg gatcactgcc tgataactca
     1861 ttttgtcagg gacttgtgca aaaagtaatg tgacagccga cattactacc agaaatgcaa
     1921 acagtctttt catgattatt tgtttttaat aattttatac gtttttacca tttggttgtc
     1981 cttcaatatt ttcaaaaaat aggtgccaga aacaagtcca ttcatgtcaa tcatggttgt
     2041 ggcatccgtc actatccggc tggcaacaaa ctttcctgtt gcatcataca actgataaat
     2101 cacttcagca gcgttatcga tttccagcac caaaaaatcc gttgttggat ttggatacac
     2161 agagcaatag aggtgttcag aaagttgttc ataaccatca acaacttccg aaatttcata
     2221 tggctgttga attccctgtg caacagagcc acttgtcgat gaattcgttg aataaactat
     2281 ctgaccaacc gtatagctta ccgaaccggc ggatccaatt gcgttaccac ctgatgccga
     2341 aatcgattca tgcatgtttt gggtgaagcc cgtcatgaaa aacaaggaca tcacaacgga
     2401 aaaaaaacat cgttttacca tgtgcagact attgaataaa aaaaaaggaa taacgccaac
     2461 aaatatatta taattattaa ttataatctg cataaaaact aatataagct tatacgaaat
     2521 tataccaaag tgatttatac atcggttcaa ttatttttgg gagactccgg aggtttcatt
     2581 gaataaacca ttccgtacct gacggcacgg gctggcgttc ttttacgtgc taccaatatt
     2641 ttgtccctac gggacaattg cagctatgtg tgctgtttcc cttgtgctcc gaattccggg
     2701 aaaatcacgc cgattttgca gatctgaaat aacgccaaaa caattgattc atcggcgcaa
     2761 ttatttttgg acgactccgg aggagtcaca tatctgtaga taagatcaaa caaagctacg
     2821 actccggagg agtcgcatat aaaatgcttc gtcttctgat aatacacgtc aatatgcgcc
     2881 ctctctgaca tcagccaaca aaacgttcaa tgaaattgct acaattgact cttttatatt
     2941 ttcatttggt attacttctc ccgctttttt tggagaaacg tccgccacat gcctttagac
     3001 gcggcgaaac gaagtcgtca ttgccgtgtt tttatgcagc caaagacagg gcacattaat
     3061 ttcccgggga caacggaaag cgtttgtaaa tgctaaaaaa gaggaaacat tgatgtcgtt
     3121 tcggtgttat ttctttttag gcgcgaagcg gtgtatcaca gcatgcacat gcacgatccg
     3181 cgcaccatca gcaaggaaga aacctggtgg agaatgaata atgtggagaa attcaaacgg
     3241 cgctgatcgt gtaattcgca gcagaaaaat gaagaggctg cggaggcaaa caccggatca
     3301 ctttttatac ctgccatcgt taggaaaaga attaacttat tgctaaattg tggtgatgaa
     3361 tttcagaatt cacatagaat tatcctacct gaggttaaca ctatttggga tcttaaccac
     3421 ttttcagaac ataacgccaa aaaccttcca aaattccccc gtcatttttc gaaatcgacc
     3481 ggaatcgcct gaaaaactcc caaaatcacc tggaaataaa caaaaaaatg acccggcgga
     3541 ggtaaatgcc gggaaaaata atgttttata tcgcatttgt caggtttgag ctgacgtacc
     3601 tgatacaaat aatgaacaag gaattcgccg cggtggaatc gcgtataagc ggagagccga
     3661 gattgttgct ttgaaggttt tccacgagat cccggcacag tgagcaaagc tgtgtctcaa
     3721 tcaatgaccg ggaactcagt ggtaaaaaga ttttggtgtc tttgtgtcct ggtggcaaaa
     3781 tcctctgcgc ctctggtccg ccttcgtcgg actaaactcc ctctgcgcga aactcatgtg
     3841 ttttctctgc tgaaagcagc ttagcctcaa cctcaacctt aacctcaacc ttaacctcaa
     3901 ccttaacctt atcctcagcc ttatcctcat cctcagcctt atcctcatcc taaaccctat
     3961 cctctgcaaa ttcccgctct ccatccgtat tttttcaaac atagcagaca aatttccaat
     4021 tttcgtcttt aacaccccta cccatttact gatattcaac agttttggtg caacctttca
     4081 gacctttgcc gtatctttgc attaaattta cagtgatgaa aaagtggtta tttgtattgg
     4141 tgctgatgat ttctttcaac ggactgaaag ccgaaggcgg cgacacgata tacgcaggcg
     4201 tgaatctttc tgaagaattt cttttataca tgcccaatgc cttttcgccc aatggcgacg
     4261 gccacaacga ttatttttcg cccgttggcg caggtattac gcttgattat tttgaaatgc
     4321 gaatatacaa tcgtcgcgga aaacttgtat atttgaccac cgatatcaac caaccctggg
     4381 acggtgacat tacaggaaaa cgttacgatg aaacctctac agaagttttt gtttatcaca
     4441 ttactgtaag aacatacaag ggcattgaac gggattatta cggccacgtt gtacgattac
     4501 cttaatacat gaaccgattc aaattcttaa aggaatttct gaatttatcc aaaaccgaaa
     4561 aagccgggat ctggcttctg ctcacaattc ttataagcat tattttgctt tccgttttcc
     4621 tccacagcac aaaaacggaa ttggctgatt ctgatttttc tgattttgaa aaacagatac
     4681 aaacatggga aagcagcatc agtagcgaca atcagcaata cagcaatgta tataacgacc
     4741 ttgatgcgcc ggcatattct gcagcaaaat caaagctaaa tccatttcca ttcaatccca
     4801 acgaacttga tgcagagggc tggaaacggc ttggcctcac cgataagcaa acaaagtcca
     4861 tattaaatta ccggcaaaaa ggcggtgaat tccgcatcaa aagcgatttt tcgaaaatgt
     4921 acaccatcag caaggaagag tttgcagtac ttgaaccatt catcgaacta cctgaaaaac
     4981 cgcagcaaag gcccgattac agcgcctcga cctatgaaaa aaaagaagtg atacgtgttg
     5041 aaatcaattc ggccgacact gctgcattga aaaaagtaag aggcatcgga cccgcttttg
     5101 cattgcgtat agttggctac agaagccggc ttcacggata tatttcgata gatcaattga
     5161 aggaagtgaa aggaatcgac tcgctgaaat ttgccgaaat ttctccgtac atgtttgtaa
     5221 acccgtatct ggtgcggaaa atcaacatca acaccgcaac ctttgatgaa ttgaaaagtc
     5281 atccctacat aggatacaac attgcgctgt cgctcatcaa ttaccgcaag cagcacggcg
     5341 attacaaggt gctcagcgat attaaaaaat cggcgctggt caccgaagac atttataaaa
     5401 aaatcagccc gtatctgacg gctcagtgat ttagacttcg ttcggcgtgg aatttatccc
     5461 gacgaaggcg ggaccatgga atgcaagcca ggtccttgtt cttgtcctcg atctaaaatc
     5521 aaaaatcacc gatcgatatt cttaaatcaa aaaaaggagg cccgtatcga tgcctccctt
     5581 ttgttttttt ccgttaaaaa agacggaacg ttcttattca aattcgacca ttaatgtgtt
     5641 ttttggaaca cgttcgccct ctttcacatg aatagctttt attttcccgc tcataggcgc
     5701 aagaatttta ttatgcattt tcatagcttc aagttccagc aaaacatcgc cttcagccac
     5761 ttcgtcgttt acagctgaaa agagctttat aatagttccg ggaatagctg aaatcacatg
     5821 tttgatattt aagggagcat atgatttccg gttgagatat ttctttgaaa accgtgtttt
     5881 gtattcgcct tgttctgtta ccagaatatc aaactgctct tcggcaattt cgggcgtttt
     5941 ggtagttttc tttgcttttc gttttgttgt taatttcata gctgcatgtt tttcagaatg
     6001 gaggaatgcc gtgtttttta gtaggacggg ttaccatttt cgaaacagaa acctcgagag
     6061 catgagcaat aaaactacgg gtttcggccg ggttgataac agcgtcaaca taaccgtaag
     6121 cagcggccac atacggattg gcaaattttt ccttgtattc ctgaactttc ttcagacgca
     6181 tttcttcagg attttcggct gcctgaattt cgctgcggaa aataatgttg gctgcccctt
     6241 caggacccat tactgcaatt tcggaggtgg gccaggcaaa cacaaaatcg gctttcaggt
     6301 ggcgcgacga catggcgatg taacctccgc catatgcttt acgcagaatg atggtaattt
     6361 tcggaacaat ggcttcgctg aatgcataca acagtttcgc tccgtgacgg atgacaccgg
     6421 caaactcctg atcaacgccc ggcagatatc ccggaaggtc aaccaatgta atgatcggaa
     6481 tattgaaagc atcgcagtaa cgaacaaaac gtgctgcttt atcgctgcta tccacatcga
     6541 gcacgccggc cagcaccagc ggctgattgg caacaaaacc aacggtatca ccttcgatgc
     6601 gtgcaaaacc aataacaata ttgggtgcaa aaagctccat aatttcgaag aaatcgctgt
     6661 catcggccag agcgcggata acttcacgca catcgtaagg tgttttcggg tcagtcggaa
     6721 ggatagatgt aatcttgtaa tttttgtttg gcttcatcgg ttcaaagcga tccgctttct
     6781 ttgtattatt ccatggaata aacgaaagca gcgcttttat ctgcgcaaaa cattcaaact
     6841 cgttacgggc aaagaaatgg gcgtttccgg taatttcggc atgaacacgg gcaccaccaa
     6901 gatcttccat tgaaatttct tcaccaagaa cggttttgat tacttcgggg ccggtaataa
     6961 acatctttga aatgttatcc accacaaaca caaaatcggt taaagccggg ctgtaaacag
     7021 cgccaccggc gcaaggaccc agaatgaccg aaatctgtgg aattacaccc gatgcgagtg
     7081 tattgcggta aaaaatctca ccataacctg ccagagagtt cacgccttcc tgaatacggg
     7141 caccaccgga atcattgata ccgataagag gcacctgcat tttcatagca tgatccataa
     7201 ttttggtaat cttcatggca tgcatgtaac ccagcgaacc gccggccact gtaaaatcct
     7261 gagcataaat acagatcgga taaccattga cggttccggt acctgtaata acaccatctc
     7321 cgggaagatg ttttttatcc atgtcaaaat ctcttccggc atgctccaca aataaatcat
     7381 attcgtgaaa tgaatccgga tccagaaggg ccagaatacg ctcacgggcc gttaattttc
     7441 ccatggctac ctgcttggct atggctttgg taccaccacc ctgacgcgca tcaagtacac
     7501 gcttccgaag gtccattgat ttcgatctga ttgacataat ttgttttttg gttttacttg
     7561 cttttcttca aaattccggc aaagtaaagc atattattgc gtaccatgtg tgaacaactc
     7621 tctgacaaat tgacagcagc caagcctcta agccaatctg gatgcactta ttaagccata
     7681 tcaacaccct cttccaatta acatttttta actttgaaaa ccccaaataa aaaaagggca
     7741 gcagaaaaaa attcgggcat tttcattttt attgtttttt tgtattacct tgcatattct
     7801 ttttacgggt taacaatatt ataggatgaa gcatttttat ctgttcttgt ttctgatggg
     7861 ggggctaatt gcagaaacag ctgaagcaca gcacttttcc tttaccccgc ctgaaaattt
     7921 tgttgcagac accctcaaca gcggttctac caactggttc aacaaagaag ccggcagtgt
     7981 tatacagatt accgaaacaa ccggaacggc cttcaacacc ttcaaggaaa atttcaatga
     8041 agagcagctt acaaacaaca atctggttat caaagaaaaa aaaccggccg gtcccgcctt
     8101 gttgtatatt tgttcatacc ggacaatggc agccgatgga agtcgcgatg ttgaattcac
     8161 tcgtgtcatt tttttcacgg gaaatgcaaa tgccgttgta atggctgtag ccaccattcc
     8221 ggccatggct gagccggttt tactggaacc aatcattcaa tcatttgtca aagatttgat
     8281 tattcgctga ttataaaaaa gtttttcaaa gggggttatg aaaagaattt taccagtctt
     8341 attgttttgt gcatcattgt ttgcttttac gcattcaaaa gcacagactt ttacggtcaa
     8401 ccaccagata ccctggagca ccgattatca gaacatgtgg ggacccaacg gaagcccatt
     8461 ttcactaaac ttcgactaca gtctgtttca ttttatgtac gacagcacca caacttttgg
     8521 acaaatcgaa aacatccttg gcggtgattt tggagctgaa ttcgatcttg atatgtacct
     8581 tgaaatgggc tctacctttt caattcatgg cttcactacc ggctgggtcg atgtggatta
     8641 ccctgtcgat atcgaactca ctttccccga ccctgggcta cccgttccgg gacaatacat
     8701 gaccgtcaat tctgactatg atgttcttcc gggctgggat ctgagctctc atttccctca
     8761 ggcaggtgtt gttacactcg atctcgattt tggcatgagc atggatttgt ccggacaggt
     8821 ttgctttatc ggatgtacac cggtcaatat tgccaacttc gatattccac tcgactccat
     8881 cattattttt gaactcaact ccattaccgg cgaagttacc tatccctgca tcaacggctt
     8941 tctgcctgcc atttgccacg acaccatact tccgcttatc atcaccaaca tcggaggcat
     9001 cggactcgat cttacggccg acatacccta tattcaaacc atcgactcgc ttggaagcga
     9061 caaatgtctg tatgcctatg gcgatgattg gtatctggag ctttcgctcg atattattga
     9121 atttctttct tttattgcca acatcattcc cccacccgcc ggaccggcca ttgcttcggt
     9181 attgtccatg ctcaatggca cctatgatct ggggtatgga atttccattg actacagttt
     9241 actttcggcc tttctcacac tcaacaatac gttgcaacag gatctgacct tctgtcccga
     9301 tgtgtggacc gaacttacct accccattgc acttgactat ttcatcacag atcccattgc
     9361 cggcgataca gtcggaacgg gaaccaatga ttccatttca ttcatggccg gacacgacct
     9421 gcatgtaaaa tggccctgtt tcggctacac tgaatttgac ccgggaatac ggcattttct
     9481 caccaatgat ttcacaaatc atacctggga ctccatggcg ttcgattttt caattcaggc
     9541 ctttgaattc accatcaatt ttccatcatt tcccgtagct cccgcttctg atcttccgga
     9601 actctgtgtt gcaataccac aaaatgacag cgctgccact cgggcgcttt gtgtcgatcc
     9661 ggtacaaaca cccgaagttt atctcgatat gagcaaactt tcattccata tcggaccact
     9721 ggtcgattac accattccgc tcggttatat tccgcttaca tggtatctga acacctggga
     9781 actcgatgga ttccacgaca ccattttccc atccgttcat cttgagtcgg gaccattttt
     9841 ccagatgacc attcttggcg acacaaccat ttgctatggc gatagtacgg gaacccttat
     9901 tgcaacaggg atcaacggtg catcgcccta ttctttccaa tggtcaaccg gcgaaaacga
     9961 ttcacacagc tggcctaccg actccatttt tgttccttct ggtctttatt ctgttacagt
    10021 aaccgatggc ggtggatgta cactaagcga cgatgtcatt gttgttgaca atccgcagat
    10081 atttgtctct ctgtccaaaa ccgatgtcaa ttgtttcgga gactcaaccg gtacaatatt
    10141 atccactgtt tcgggcggaa cacccggagt tgctcccggc tacaactggc tctggatgcc
    10201 aggtaacgca agctctcaaa acatcaccaa tattcctgca ggattctaca cccttgttgt
    10261 taccgattcg ctcggatgca cggcaagcga taccatcacg gtaaatgagc ttcacccaca
    10321 tccaccggtc gatgtcatgg ctgatccgtc cgttggttgc caacccctgc ttgtacaatt
    10381 cgacgaaatc aatccttcaa caggcaacag ttataactgg gactttggcg acaattccac
    10441 attcagcatt gccgaagatc cgctccacga atacgataca gctggcacat tcagcgtaac
    10501 gcttactgtt acgaatgagt gggattgcgc tacaacagaa acctacaacg atctgataat
    10561 ggtgtatccc aaacccgctg catctttcta tgctacaccc gatctggttt acaccagcga
    10621 agacccatcc atgaccattg attttacaaa cacaagcagt ggcgaaattg cctggttatg
    10681 gaactttgcc gactcaacct cagcaaataa tacatcaaca ttcgaaaatc catctcattc
    10741 attttctgac gaaggccttt tcaacgtaat gctgattgtt accagcgatc tgggctgtat
    10801 ggatacagca tgggtttccg ttgaagtcat tgacgacgtg cttgtttttc caaatgtgat
    10861 tacaccaaac aacgacggat tcaacgatgt gtttgaaatc gtcaatgtgg aaaaatttcc
    10921 tgaatgcaag ctgaccgttt tcaatcgttg gggcaacatc gtttatatgg catcgcccta
    10981 ccggaacgac tgggatggag aaaactcagc tgacggaaca tatttctatg tgttcattta
    11041 tggaaaagat tcgaaagagt atcgtggttc actaacgatc atcagataat ttggaaaaat
    11101 attgtacttt tgaaaaaaaa agagtatgaa aaacctgttt gcattctttt tcctggcact
    11161 tttttctgtg acattgcttg cacaggaaga agatctcgaa ctatcaaacc gctttggaaa
    11221 agccgaccgg ctaatgattg atctttataa tgatacatgg cttggagcgc ctgacagtat
    11281 tgatttcaaa ggatacaacc ctggtgtggc tataaatctg atgaaagact tcccaatggg
    11341 agccaccaat ttcagttttg ccattggagt tggaatcggc acacacaaca tgagatacaa
    11401 tgctactatt tttaacgatc ccgttacttt cgaaaattat tttgtaaacc gtctcgacac
    11461 gccatcccaa tacaaaatgg ttctttcttt tattgatatt cctgccgaac tacgcttcag
    11521 aacaaaacgt gacaatgttt tccgcattgc aataggtggt aaaatcggct acaacatcaa
    11581 caaccatatc aaacatgtgg atgatgattt caaaatcaaa acatacaaca cggaatacat
    11641 caataaactc cgttatggtg ttacagcacg catcggatat aaaatgttca atctgtatgt
    11701 gtattatggt ttatcaacat tgtttgaaga aaaacatggc ccagaaatgg cgccactatc
    11761 aataggaatt tctctgattc cattctgata aaaaatccta taaaaaaagg tctcccaaaa
    11821 agagaccttt ttttatgcag ggaatttttt ctaattacgc cattcataat aataaaccgc
    11881 tttaaaacca catcccagat taactgtcag ctttttcatc tccttatctt ttgtcccttt
    11941 cacaatgggt ttttcgctca caatatcagc actgaaatcc tttttaaaat aattttctcc
    12001 ggcaggattg cgcaagaaat tgccgggata accttgttca aaaaatacag attgagcttc
    12061 gggggtccag gatgcaaatg ttgaaatggt tttaagggtg tatttcattt taaaagaccc
    12121 tccagcagcc agatatgctt gcggatcagt ccaggtaaca ttataggttg tacccgccaa
    12181 caattttcca ctgctctggt cgttgcggtt ttttgttatc tgaatatttc ccttgctacc
    12241 ggtaaatttt gatgcatcga ccatataatc gactgtaccg gccaagggtt tcaccgacga
    12301 agagcttccg tcaataaact cgtattttac aaaataccaa ccaggttgat taccaccgac
    12361 ggcctgtgat gtagtattca tagaggttcc ggctgtgtaa tcaggtagcc attcatatac
    12421 atattctgct ttgaatccac aaccaagatt tacaataaac tttttttgtt cattgttttt
    12481 agtgcctttt acaatgggtt tttcactaac aatcgcagca ctgaaatcct ttttaaagta
    12541 attttctccg gcaggattac gcaagaaatt gccgggataa ccttgctcga aataaacgga
    12601 ttgagcttct ggagtccagg atgcaaatgt cgaaatggtt tttaatgtgt attggatttt
    12661 cattttttca ccaaccacaa caatatcagc cggatcagtc cattccacct tataagtaac
    12721 accggccaat agtttcccgg tgcttttgtc attgcggttt ttcgttatct gtatgcttcc
    12781 ttttccgcca atgaatttaa tggcatcaat catataatca gccgttccca tcaatggttt
    12841 ttcgctcgac gatgtgccat cgacaaactt gtatccaacc agtttccatg cgccctgcgc
    12901 aaagatgaag tttacggata acaacaggat tgaaaatatc agaaactttt tcatagcgtt
    12961 atattttagg gtttattgat tgcatgtatg aaacaaatat aaacaatttt ttcaattaat
    13021 tgatttcgaa caaagatcaa gataatgaaa ttcaaaaaaa acaggcggag attatccgcc
    13081 tgccaaacaa tgaataatgt ttgattttta tagagcgcct tcgtcgtaag ctgtttcgcc
    13141 atgtattgaa acatcaaggc cttttcgttc atcgtcttcg ctgaccctta ctttcacaac
    13201 catgttgatt aaaatcagca taatataggt gaaaataaaa gcgtaagcag cagcagcagc
    13261 aacagccaca atttctttaa aaaagaaatc accgccaccg aaatacaaac cgtcggcgcc
    13321 ggccgcattc accgccgatg aagcgaatac acccagcagt atggtaccta ttacaccgcc
    13381 gattccgtgt acgccccaca catcaagcgc atcgtcccat ttcattttgt ttttaagctg
    13441 aaccgccaga taacaaacgg caccggcagc gactccgata ataggcgagg cccacaacgg
    13501 aacaaatccg gcacaaggtg taatggttgc cagacccgca atggatccgg tcagtagccc
    13561 gataaatttg ggccggccca catgaatcca ttcaataact acccaggcca ccgtggcaaa
    13621 tgaagctgca atatccgtat tcagaaatgc cagcgaagta atattatcta ccgccacttc
    13681 gctgccggca ttgaatccat accagccgaa ccacagcaat ccgcttccga ttgctacaag
    13741 cggaatgctg ttgggggtgc tgtttttgtc aacgcgggca ccaacataga atacagaggc
    13801 cagcgcagca aaacctgctg tggcatgcac cacaataccg cctgcaaaat caagcacacc
    13861 ccattcagcc agcatgcctc ccccccagat catatgtaca aacgggtaat acaccaaaat
    13921 ctgccacacg gttaaaaaga tcatatatga tttgaaagta acacggtttg caaatgctcc
    13981 ggtaataagt gccggtgtga taatggcaaa catcatctga taggcaatga aaacgaattc
    14041 aggaattttc ccgtttgtgt acatcgaatc catgccgact ccggccagaa aagccttgtc
    14101 gaagtttccg aaaatgccgc ctgcgccacc gctgaaacaa agcgaatatc cgaatgcaac
    14161 ccataaaacc gttgtccagc caagcgaaac aaaactctgg atcataatac ccagaatgtt
    14221 tcttttggtg gccaatcctc cgtagaaaaa tgccagaccc ggagtcataa gcattacaag
    14281 gcttgttgca agcaacataa agccggttga acctgtgtca atcatttttt tattctccta
    14341 ttttttttaa agtgtgaatc cgaaaacaag aaatacattt tctgccatgg ggtttaccac
    14401 aaatgaggtg gaaagcggca atgtgaaatc attggtgatt ttgacctctt ttttcacctt
    14461 gattccaagg ttgacaaatc ccattgtgtt tccgtaaaac ccgcgcgtga tgccggacag
    14521 cttatcattg tcggttggct tgtttggagt gaatccggca aacagatcat aatcggccga
    14581 tttgcatgtg ccgctgtagc acaattcaac gtatgttgag taaaacaatt tgccatcggc
    14641 atgacgtgtg tcggcgccat acacattggc ggcaacgagc agtgataatg gaagatcttc
    14701 ggtgcctttc catttaatgg ttccttcaaa tatatgtccg gtcagggtat cattatattc
    14761 aaagtaattg ttgaaagcac cgggtgtttc gttcggaaag aaataatcgg tgatggtaaa
    14821 atccactttg tctttcaatg tgtaggttag aaacagatct gtttcctgcg cctgcgaaaa
    14881 attgttggtg gcaaatgcac cccaaacgcc gaaaatgaag ttgaatccgg tcagcgagat
    14941 agccggctga atactgggcg atgcgccgaa atcggttccg cgccatacat agcggctcat
    15001 gatatcggct ccgctgttga actttacttt ggaactgtcc tgtgctgaga ttgacagggg
    15061 catcaacgcg gcgaaaatca acagaatggc aagtcggttt ttttgcttca tatgctttgt
    15121 tttaattcgc tacaaagcaa taaaacccgc accaaatgca gaataccgga ttaacgaaaa
    15181 agcatgcgtg tattacggaa tcttcactag gtgaatcacg tatttttcaa aaatcagatt
    15241 tcgattattt tattcccgat ggtatattta atcagctcaa ctattcctga acatcgacac
    15301 tttaatttcg cccattctaa aaaaaaacag atattatgaa ttttgattaa ttcaggccgt
    15361 ttatgcgggt ttaccagcac tataaattgc gttttttcta aaagaatact gacatccgga
    15421 ggatgtcaaa caataatagc gtccggcggg cttgtcatca cgtggaacgt gacaagctgt
    15481 tttacgtgaa gccggatata ataccaaagt gatttataaa tcggttcaat tatttttggg
    15541 agactccgga gga
//
LOCUS       DAOPKE010000063        15243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4086_length_15243_cov_12.562418, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000063 DAOPKE010000000
VERSION     DAOPKE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_15243_cov_12.562418"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /locus_tag="PKY63_08755"
     CDS             complement(<1..2532)
                     /locus_tag="PKY63_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF00742.1"
                     /translation="MSHLNKLFFVCFLAFVLGFLRPEPLLAQNNAVVFGKVSDDRGRP
                     LELVNISIMGVPGGTSSDGAGLYELAIPANKNIIVAFSILSYSVEFDTVNLAPGQRFE
                     LNVVLSDKATLLDPIDVTAHYDMAGGIIRLDPRTSVLLPTYGGIETLVKLIGMGVSSG
                     NELSSTYSVRGGNFDENIVYANDIEIYRPFLVRSGQQEGLSFLNSSLVNSVSFSSGGF
                     EARYGDKMSSVLDVQYKRPDSLRGDAHVSLLGANVHIEGKGPKGLMTFLSGFRYKSTQ
                     YVLNAMETKGDYKPSFLDWQNYFTWQLSETSELSILGYMSSNKYNLIPETRETTYGTV
                     NQAYRLTIYFDGMESDRFTTFLGGARFQHYPNKDLLLKYIVSGYSSLEDETYDIQGQY
                     WIGLLESDMGSDQFGEVAENMGVGTFIDHARNYLDVNVFSAEHKGMLEKDKYYLQWGV
                     KTQHERVFDEIWQWNYIDSSGYSLPHVSDSVGYTGIQPYQNLAISDLLISDLQLTSMR
                     YSGYIQNGWEWNVDSIRYNIIGGVRSSYWDVNQQLIVSPRAAFSMKPNWDRNYLFRFA
                     TGMYQQPPFFRELRDMRGNLHTDVKAQQSIHFVAGFEHTDSLWNRPFRVVGEVYYKAL
                     SNMIPYVIDNVRIRYLPEYRSHGYATGIDLKMNGQFVPDAESWVSISIMQTREDIEGD
                     FYNIYLNSDGDTINSASINNVAVDSIRIEPGFIPRPADQRVNVSLFFQDYLPNNPSYK
                     MHLSLVFGTGLPFGPPNSDKYKQTLRMPPYRRVDIGFSKQLAGYQHPDKPAKVPRNLK
                     FIRNAWLSLEVFNLLNINNTVSYIWVTDVENNRYAVPNYLTKRQLN"
     gene            complement(2516..3187)
                     /locus_tag="PKY63_08760"
     CDS             complement(2516..3187)
                     /locus_tag="PKY63_08760"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF00743.1"
                     /translation="MSRILILLFFLVSSFALMAQNDESGNLVQFSGVVVSADSLKPLP
                     FTHIIIKNTHRGTVADFFGFFSFVAEKGDLIEFSSIGYKKSYYKIPDTLSGSRYSLIQ
                     MMQTDTLLLTETVIYPWPTPDQFKQAFLQTRPPEDDYDRALKNLTLAELKERAAEMPS
                     SGSMNFKNQIDLTTSRLYYAGQLPPNNLLNPLAWSQFIKAWKNGDFRRKDPKDQEYYY
                     NEPLK"
     gene            complement(3288..3524)
                     /gene="rpmB"
                     /locus_tag="PKY63_08765"
     CDS             complement(3288..3524)
                     /gene="rpmB"
                     /locus_tag="PKY63_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPF00744.1"
                     /translation="MSRVCQLTGKKRMSGNHVSHSNIKTKRRFYPNLQEKKFFLPEEN
                     QWITLKLSTSAMRTINKKGISAVMKELIANGYIK"
     gene            complement(3596..4135)
                     /locus_tag="PKY63_08770"
     CDS             complement(3596..4135)
                     /locus_tag="PKY63_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00745.1"
                     /translation="MKNRFLFLLLIALAACSGGRQQLLDEISEKEAIVTKSYEDVLDK
                     STAESLRQDYWTFADDYPDDSLAPKFLHKAAELSLAIDMTKDAVNTLDTLIERYPEYQ
                     FLPDALFFKGFIQENHMKDLAAAKKTYEVFLNRFPKHELAAQVAVTIENIGKSPEELV
                     QEFMARTNQPDSTASDSVN"
     gene            complement(4135..4704)
                     /gene="def"
                     /locus_tag="PKY63_08775"
     CDS             complement(4135..4704)
                     /gene="def"
                     /locus_tag="PKY63_08775"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPF00746.1"
                     /translation="MLLPVVAYGHPTLRKRSENISPDYPDFKKFIDDLTETMYVSDGV
                     GLAAPQVNRNIRVFVIDCNPFKERYPEADGVKGLFINAEMIEQTGESWFYNEGCLSIP
                     GIHEDVSRKKTIKIKYLDENWVEHTKTYSGIVARVIQHEYDHLEGVLFTDHLSPMRKL
                     LLKGRFNDITNGKVDVDYKMIFPGKQKKR"
     gene            complement(4708..5124)
                     /gene="ruvX"
                     /locus_tag="PKY63_08780"
     CDS             complement(4708..5124)
                     /gene="ruvX"
                     /locus_tag="PKY63_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPF00747.1"
                     /translation="MGRIIAIDYGSKRCGIAVTDELRMIASPLDTVSGTALLAFLQKY
                     CSQNKVDIIVFGKPTDMSGKPSESEKQITPFINQLKKAMPEMQIDRWDERFTSKMALQ
                     AMIAAGSKKSDRREKGNIDKVSAAIILQDYLTFKNF"
     assembly_gap    5174..5273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5523..5609)
                     /locus_tag="PKY63_08785"
     tRNA            complement(5523..5609)
                     /locus_tag="PKY63_08785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5573..5575),aa:Ser,seq:gct)
     gene            complement(5663..7537)
                     /locus_tag="PKY63_08790"
     CDS             complement(5663..7537)
                     /locus_tag="PKY63_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPF00748.1"
                     /translation="MKNVFTDRFIPRWAILLFDLCIVLFSVYISFQVRFNFKVPSHEI
                     HFMEISFLVILATRLLLFLFFGTFKNIIRYTSTTDAIRISATIILGSLLFVIGNVVSY
                     YALGRPNLVPYSIVIIELMMTAGLILGYRILLKVAFLEMRAPSRDKTWVVIFGAGESG
                     IITKRAIDRDAGTKYKVLAFFDDDIRKAGKKIEGIPIYNAEKLEETLNQNQVAHVILS
                     IQTLSPARKTEITNLCLQYKTKVLVVPPVRRWINGELSFKQIRKINIEELLERDVIQI
                     SNDKVRQDIAGKIVLITGAAGSIGSELVRQVCEFHPAMLLCVDQAETPMFHLQNELDQ
                     RRFKDYKIIIGDICDEQKMRLVFNMYKPAIVFHAAAYKHVPMMELNPAEAVRNNVIGT
                     RVLANLAMEFKTQKFVFISTDKAVNPTNVMGASKRIAEMYVQAHDKKSFTRFITTRFG
                     NVLGSNGSVIPLFRKQIERGGPVTVTDTEVTRYFMTIPEAVSLVLEAGTMGQGGEIFI
                     FDMGKSVKILDLAQKMILLSGLTLGKDIQIEFTGLRPGEKLYEELLADKENTLPTYHE
                     RIMIARVENRDAAAIESDVNVFPLLLNDNNETEIVRKMKSIVPEFQSRNSIFEQLDKK
                     "
     gene            complement(7782..8885)
                     /gene="gcvT"
                     /locus_tag="PKY63_08795"
     CDS             complement(7782..8885)
                     /gene="gcvT"
                     /locus_tag="PKY63_08795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPF00749.1"
                     /translation="MKTTAFTKFHEEMGAKMVEFAGFYMPLQFEGVSVEHETVRKGVG
                     VFDVSHMGEFWVQGPKALDFIQHMTSNDASVLVDGKVQYSCLPNGKGGIVDDLLVYRI
                     AADNYLLVVNAANIDKDWNWMAPHAEKFGLKVGVDFRNESDDYAQLAVQGPLALKAMQ
                     KLTATTVTDMEYYTVKVIEFAGVKDVIFATTGYTGSGGCEIYMKNADAAKIFKAVFEA
                     GAEFGIKPIGLAARDTLRLEMGFCLYGNDIDDTTAPIEAGLGWITKFTDTKNFIDKDY
                     NLALKNGGAKRRLVGFEMIDKGIPRHEYEICKADGTVVGHVTSGTMGPSVKIGVGMGY
                     VPLEMAKTGSEIYIKVRDKLLKAAVVKTPFYKG"
     gene            complement(9098..11212)
                     /locus_tag="PKY63_08800"
     CDS             complement(9098..11212)
                     /locus_tag="PKY63_08800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPF00750.1"
                     /translation="MKNIFSLKAMPYVVMILIAGCGGADKKNKDNPFFSEFNTQFGVP
                     PFDKIKFEHYQPALEEGMKQQMLEIDSIANSQDEATFENTIAKYDASGQMLSRVSSVF
                     YNQLEANTNDSMQNLAQEMAPKMSKHSDDIMLNEKLFKRIQTVYNNRSGLNPEEKKLT
                     EIIYLDFVRSGANLDKKGKERLREINEKLSLLELQFGDNVMADNNAFQMVIEKNEDLA
                     GLPQSVVDAASEAAKQNKMEGKWLFTVDKPSLIPFLQYSERRDLREKIYKGYFMRGDN
                     NNKNDNKQNITQIVSLRLERAKLLGYLNHASFILDRNMAKTPEKVYDLLQTVWDAALP
                     VGKAEAAEMQKMIDKEGGKFLLEPWDWWFYAEKIRKEKYDLDEEMLRPYFSLDNVREG
                     IFLLSNKLYDISFKKIDNLPVYHKDVTVYEVLDTNNQHLAVLYMDFHPRASKRGGAWC
                     TSYRDQYVRNGQNITPVVSIVMNFTAPTANSPALLNLDETLTFFHEFGHAIHGFFSNV
                     KYKRLSGYVPRDFVELPSQVMEHWARHPEMLKLYAKHYKTGEVIPDELIAKIEKSGNF
                     NQGFATLEFIAAAVLDMDYHTLENASNLDILAFEKASMDRLGLMKEILPRYRSTYFNH
                     IFAGGYSAGYYSYVWSEVLDSDAFEAFEESGDIFNKELAAKFRHEVLAPGGKRDPMEM
                     YVAFRGREPLVDALLRNRGFKK"
     gene            complement(11388..11969)
                     /locus_tag="PKY63_08805"
     CDS             complement(11388..11969)
                     /locus_tag="PKY63_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00751.1"
                     /translation="MKIQHIVLLAIIMGSMNFCKHKKEVVRETSDSIVAEEQFRDTAV
                     AVDPAEALIVDNNASNQDGRNWKKEEIASGTAFYKLRYCGGARPTPDIEKEFNQEHLL
                     VSQKIKFVNVDDKSDFVIVSTDENGCFTVVLHQGSYDYFLVYTEGSKWPPNPGCQKYY
                     ERIYGKITVGDGSWSGYKLLYSFPCDPCSPPKP"
     gene            12171..13082
                     /locus_tag="PKY63_08810"
     CDS             12171..13082
                     /locus_tag="PKY63_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPF00752.1"
                     /translation="MIEQIRLDKVKWLHILYPSDEDYNLLINEYHFHPLDIEDCKTRA
                     QRSKIDIYDDYYFLILHFPYFDKINRFIKVKEIKIFWGEDFIITIGSAHWVIRDLFEE
                     LRRQINDGQIDEDLSSSDKLLYRILESLMKETQVILQRTDTEIDLINKEMFSRNADKI
                     IEKISITRKNVILLNTTFKPQLRLFHKFESGEVKGFAEDMEDYWGNILDYYQKNWDMI
                     EDDGELLEGLSKTFDSMQANRTNEIMKMLTFISTTLLPMTFITGLYGMNVALPFGEHP
                     FAFSMIIGIMALIVIFLILYFKRKRWM"
     gene            13095..14003
                     /locus_tag="PKY63_08815"
     CDS             13095..14003
                     /locus_tag="PKY63_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF00753.1"
                     /translation="MRGNKGVSLLFMLILVLTWGSSFILMKKALVYFPSNIVAAYRMT
                     AAFSIMLIPALIYIFKMPFKTFLLLTLSGILANGLPAFLFAYAQTGIESYVAGILNST
                     TTLFTLIIGIIFFSYKARLLSIAGVVIGFAGIVGLLAFAGDKTLSFNFSYGIYILIAT
                     TSYAININFIKRWLSNVPTLSMVSVVFFITGLFSTIYLFGYTDFIQITKTTAGSGFGI
                     LYMSILGIVGSAMALLMYYHLIKIADILFVASVTYLMPVVSTLWGLGDGEVLGLAHIA
                     FILLILIGVFMVNYHDLMGKIKQRYK"
     gene            complement(14074..14415)
                     /locus_tag="PKY63_08820"
     CDS             complement(14074..14415)
                     /locus_tag="PKY63_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00754.1"
                     /translation="MKKILLVSVFFVICLAMSAQQTKHVEKIRILETGTAVLNNGQCQ
                     ISLNEEIDPANYYIVLTPVGKPAVLYIANQESKQFVVKSDVETTAEFQYMVIEKRRKE
                     VLDTNNTKSGK"
     gene            complement(14435..15037)
                     /locus_tag="PKY63_08825"
     CDS             complement(14435..15037)
                     /locus_tag="PKY63_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00755.1"
                     /translation="MYITNNLGIASAAPTEKLDVTGNIRFSGALMPGNDPGTSGKVLK
                     SNGAAVAPTWISAVTPDNIYTVESTAALEITSSTFTVIPGESITVNGLNPGDRVLIWF
                     GGNMYMDGADYNIVDVALHINGTMAVVGGFVRTAIDWDYAELSWQNYSAIARYNVTVA
                     GDYTFDVRARATLGVSESIFIGGDATQAAESVLQVFVLRN"
BASE COUNT         4528 a         3326 c         2980 g         4309 t
ORIGIN      
        1 attcagttgc cgttttgtga gataattcgg cacagcgtag cggttgtttt cgacatcggt
       61 gacccagatg tatgaaaccg tgttgttgat gttgagcaga ttgaaaacct caagactcag
      121 ccacgcattg cggatgaact tcagattgcg cggaactttt gcgggcttgt ctgggtgctg
      181 atatccggcc agttgttttg aaaaaccaat gtcaacgcgc ctgtagggcg gcatgcgcaa
      241 agtctgtttg tacttgtcgc tgttgggcgg gccaaacgga agtccggtgc cgaaaaccag
      301 actcagatgc attttgtagc tggggttgtt cggcagataa tcctggaaga aaagactgac
      361 attgacgcgc tgatcggcag gacgcgggat aaatcctggt tcgatgcgaa tgctgtcaac
      421 agcaacgttg ttgatagatg ctgagtttat tgtatcgcca tctgaattca gataaatatt
      481 gtagaagtcg ccttcaatgt cttcgcgggt ttgcatgatg ctgatgctca cccaagattc
      541 ggcatcggga acaaactgtc cgttcatttt caaatcaatc cctgttgcgt agccatgcga
      601 gcggtattcg ggcagatagc ggattcggac attgtcaatg acatagggaa tcatgttgct
      661 caaggccttg taatacactt cgccgacaac tctaaaaggc ctgttccata gcgaatcggt
      721 gtgttcaaac ccggcaacaa aatgtatgga ttgctgggct ttcacatcgg tatgaagatt
      781 gccccgcata tcacgcagct cgcggaagaa gggcggttgc tgatacattc ccgttgcgaa
      841 acggaaaagg taatttctgt cccagtttgg tttcatgctg aaagccgcac gtggactcac
      901 aatgagttgt tgattcacat cccagtaaga gctgcgaacg ccaccaataa tattgtatct
      961 aatactatcc acattccatt cccaaccatt ctgaatataa ccggaatagc gcatcgaagt
     1021 caactgaaga tcggaaatta aaagatcgga tatcgccaga ttctgatatg gctgaattcc
     1081 ggtatagccc accgaatctg agacatgagg caaagaatag ccagaagaat caatgtagtt
     1141 ccattgccaa atctcatcaa aaacccgctc atgctgagtt tttacgcccc attgcagata
     1201 gtatttatct ttttccagca ttcctttatg ttctgctgaa aacacattta catccaaata
     1261 atttctggca tggtcaataa atgtgcctac gcccatattc tctgccactt cgccaaactg
     1321 gtcgctgccc atgtccgatt ccaaaagccc gatccagtac tgtccctgga tgtcgtaagt
     1381 ttcatcttcg agcgatgaat atcctgaaac aatgtatttc aacaacagat ctttattcgg
     1441 gtaatgttga aaacgtgctc cgcccagaaa tgtagtgaaa cgatccgact ccattccgtc
     1501 aaaatatatt gtaagacgat aggcctgatt cacggtacca taggtagttt ctcgggtttc
     1561 agggatcaga ttgtatttgt ttgagctcat gtacccaaga atactgagct cagaagtttc
     1621 ggacaattgc caggtaaaat aattctgcca gtcaagaaat gagggtttat aatcgccttt
     1681 ggtttccatg gcattcaaaa catattgcgt gcttttgtaa cggaatccgg aaaggaaagt
     1741 cataaggcct ttggggcctt tgccttcaat atgcacattg gcgccaagca agctcacatg
     1801 tgcatcgccg cgcagcgaat cggggcgttt gtactgaaca tcgagtaccg acgacatttt
     1861 gtcgccatat ctggcttcaa agccgccgga tgaaaaagag accgaattca caagacttga
     1921 attgagaaag ctcaacccct cctgttgacc gctacgtaca aggaatggcc ggtaaatttc
     1981 tatatcattg gcataaacga tattctcatc aaaatttcca ccacgaacag aataagttga
     2041 agacaactca tttccagatg agacccccat accgataagc tttaccagcg tttcaatacc
     2101 gccataagtg ggcagcaaaa ccgaagtccg cggatcgagg cggataatac cgccagccat
     2161 gtcgtaatga gcggtaacat caatcggatc gagtaaagta gctttatcgg aaagaacaac
     2221 attaagttca aagcgctgtc caggagcgag attaacagta tcaaactcga cagaatagct
     2281 cagaatggaa aaagcgacta taatattttt attggcagga atagcaagtt catacaaacc
     2341 ggccccatca gacgaagtac cgccaggcac acccataatg gaaatattga ccaattcgag
     2401 cgggcgtccc ctgtcatcag aaacttttcc aaataccact gcattattct gagccagcaa
     2461 aggttcgggt ctcaaaaatc ccaacacaaa agccaggaag cacacaaaaa acaacttatt
     2521 taagtggctc attgtaataa tattcttggt ctttgggatc tttacgtctg aaatcgccat
     2581 ttttccaggc cttgataaac tgcgaccagg ccaggggatt taaaagattg tttggtggca
     2641 actggccggc ataatacaat cggctggtgg ttaaatcaat ctgattttta aaattcatgg
     2701 aacccgaaga aggcatttcc gcagctcttt ccttaagttc ggcgagggtc aggtttttca
     2761 gagcacggtc gtaatcatct tcaggtgggc gcgtttgcaa aaacgcctgc ttaaactgat
     2821 cgggtgtcgg ccatggataa atgacggttt ctgtcagcag cagcgtgtcg gtctgcatca
     2881 tctgtataag cgaataccgg ctacccgaca atgtgtcagg gattttgtaa tacgattttt
     2941 tatatccaat cgagctgaat tcaatcaggt cgcctttttc agccacaaaa gaaaagaatc
     3001 cgaaaaaatc ggccactgtt ccacgatggg tatttttaat aataatgtga gtaaaaggca
     3061 aaggtttaag actatctgcc gaaaccacaa cgccagaaaa ctgcacaaga ttgccggatt
     3121 catcattctg cgccatcaag gcaaaggatg acactaaaaa aaacaataat atcagaattc
     3181 ggctcatgca gggtaattaa cgaaatacgg gcctctttag tatgcaacaa taaccaaaac
     3241 aaaaaaagct gccgtaagca gcttcaaatg tacaaagtaa aattcattta tttgatataa
     3301 ccgtttgcaa tgagttcttt cattacagca gaaatacctt ttttattaat ggtacgcata
     3361 gcagaggtag aaagcttcag cgttatccac tgattctctt ccggaagaaa aaacttcttt
     3421 tcctggagat tgggatagaa tctacgcttg gtcttaatat tagagtgaga aacatgattt
     3481 cccgacattc tcttttttcc ggtcaattgg catacacgtg acataacgat actttttttg
     3541 ggactgcaaa agtccgactt ttttttgaaa caaccaaata attgggcaaa tatttttaat
     3601 ttactgaatc tgaggccgtt gaatcgggtt gattggtacg ggccataaat tcctgaacca
     3661 gctcttcggg cgatttcccg atattttcaa ttgttacagc cacctgcgct gctaattcat
     3721 gtttggggaa acgattgaga aaaacctcgt atgttttttt ggcagcagcc agatccttca
     3781 tgtgattttc ctgaataaac cctttgaaaa acaaggcatc gggaagaaac tgatattcag
     3841 ggtaacgctc aatcagcgtg tccagcgtat taacggcatc tttggtcatg tcaatagcca
     3901 acgacaattc tgccgcctta tgaagaaatt tcggtgccaa agagtcatcg ggataatcat
     3961 cggcaaaagt ccagtaatcc tgccgaagtg attctgcagt tgatttgtca aggacatctt
     4021 catacgactt agtcacgatg gcttcttttt cggagatttc atcaagcaat tgctgacgac
     4081 cgccagaaca agctgctaat gcgataagca ataagaagag aaatctgttt ttcattatcg
     4141 ttttttttgt tttccgggaa aaatcatttt ataatcaaca tcaacctttc cattggtaat
     4201 atcgttgaaa cgacctttaa gcaacagttt tcgcatcggg gataaatgat ctgtgaacaa
     4261 aacaccttcc aaatggtcgt attcatgctg aatgacacgc gccacaatac cagaatatgt
     4321 tttggtgtgt tccacccaat tttcatcaag atacttgatt ttaatcgttt tttttcttga
     4381 aacatcttca tgaattccgg gaatactcaa acaaccttcg ttgtaaaacc agctttcacc
     4441 ggtttgttca atcatttcgg cattaataaa aagacccttc acaccatcag cttcggggta
     4501 tctttcctta aaaggattgc aatctatgac aaaaacccgg atattgcgat ttacctgcgg
     4561 tgcagccaat ccaacaccat ccgaaacata catggtttca gtcaaatcat caataaattt
     4621 tttaaaatcc ggataatccg gagaaatgtt ttccgaacgt tttctcaaag taggatgtcc
     4681 atatgcaaca actggcaaca acatgattta aaaattttta aaagtcagat aatcctgaag
     4741 aataatggcc gcactcactt tgtcaatatt tcctttttcg cggcgatctg atttttttga
     4801 gccggcagca atcattgcct gcaatgccat tttgctggta aaacgctcat cccaccggtc
     4861 aatctgcatt tcaggcatgg cctttttaag ctgattgatg aatggagtaa tctgcttttc
     4921 gctctctgat ggctttccag acatatcagt tggtttcccg aaaacgatga tatcaacctt
     4981 gttttgagaa caatacttct gtaaaaacgc cagcagcgca gtacccgaaa cagtatcaag
     5041 cggcgatgca atcattcgta attcatccgt tacagctata ccgcaacgtt tcgaaccata
     5101 atcgatggct attattcttc ccatttgcag atgcaaaggt acgaaaatac aagataagaa
     5161 atacgaaata agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgaaata
     5281 agagatgcga aacaggaaat aggaattgat tgttgcgcta tactatatgc tcgcagcggc
     5341 tggattacgg ctgcgctgcg cttgccgtga tcccgttgag ggggggtcaa cctttcatat
     5401 acattttccg cgagcggaaa atgtatattc atttaagggt atgtgttgct gcgagcaagc
     5461 tcgcgcaaca catgccctta aatgaaaaaa gccctgcttt gcagggcttt tatgaaaggt
     5521 tgcggagagg gagggattcg aaccctcggt acccttacga gtacgtcagt ttagcaaact
     5581 ggtggtttca gccactcacc cacctctccg ggttaatact gaaaggagtg caaaggtata
     5641 aaaaagtttt tttaataaag gattattttt tatcgagctg ttcaaaaatt gagttacgac
     5701 tttgaaattc aggcactatc gatttcattt tccgcacaat ttccgtctca ttattatcat
     5761 tcaaaagcaa cgggaaaaca tttacatcgg attcgatagc tgctgcatcc cggttttcta
     5821 cacgcgctat catgattcgt tcatgataag tcggtaaagt gttttctttg tcggccagaa
     5881 gttcctcata aagtttttcg cctgggcgaa ggccagtgaa ttcaatctga atgtcctttc
     5941 ccaacgtaag cccgctcaat aatatcattt tctgagcaag atcgagaatc tttaccgatt
     6001 tacccatgtc gaaaataaaa atctcaccgc cctgccccat tgtcccggct tcaagcacca
     6061 gactaactgc ttcggggata gtcataaaat acctggtaac ttcggtatcg gtaacagtta
     6121 cagggcctcc tctttcaatc tgttttctga acagaggaat caccgaaccg ttggaaccaa
     6181 gcacatttcc aaatcgggta gtaataaaac gtgtaaagga ttttttatca tgtgcctgca
     6241 catacatttc tgcaatccgc tttgaggcac ccatcacatt ggtaggattc accgccttgt
     6301 cagtggaaat aaaaacaaat ttctgagttt taaactccat agcgagattg gccagcacac
     6361 gtgttccaat cacattgttt cttacagctt cagcaggatt taattccatc attggaacat
     6421 gcttgtaggc agctgcatga aaaacaatag ccggcttata catattaaat accagacgca
     6481 ttttttgttc atcgcagatg tccccgatga taattttata gtctttgaag cggcgctgat
     6541 caagctcatt ctgcaaatga aacatcggtg tttcggcctg atcgacacac aaaagcatag
     6601 ccggatgaaa ctcacagacc tgcctcacca actcgcttcc gatagaacca gccgcgccgg
     6661 ttatcagaac aattttaccg gcaatatcct gtctcacttt atcattcgaa atctgaataa
     6721 catcgcgttc cagcaattct tctatattaa ttttacgaat ctgtttaaaa gacaattcac
     6781 cattaatcca acgccgcacc ggaggaacaa ccagtacttt tgttttatac tgcagacaca
     6841 agttggtgat ttcagttttt cgtgccggcg aaagagtctg aatggataaa atgacatgag
     6901 ccacctgatt ctgattcagc gtttcttcaa gtttttcggc attgtatatg ggaattcctt
     6961 caattttctt tccggcttta cggatatcat catcgaaaaa agcaagcact ttgtattttg
     7021 ttccagcatc tcggtcgata gctctttttg taataatacc actttcacca gccccaaaaa
     7081 tcactaccca tgttttatct ctagatgggg cgcgcatttc gagaaatgca accttaagca
     7141 aaatgcggta tccaagaatc aacccggcag tcatcatcag ctcaataata acaatggaat
     7201 agggaaccaa attcggcctg cccaaagcat aatacgaaac aacattgcca atcacaaaaa
     7261 gcagactgcc aaggatgatg gtcgctgaaa tacggattgc atcggtagta ctggtgtatc
     7321 ggataatgtt tttaaaagtt ccgaaaaaca aaaacagcag cagacgagtc gccaatatga
     7381 ccagaaaact gatttccatg aaatgaattt catgtgacgg caccttaaaa ttgaaacgaa
     7441 cctgaaagct gatgtaaacc gaaaaaagaa ctatacaaag gtcgaacaga agtatagccc
     7501 agcgaggaat gaaacgatca gtaaaaacgt ttttcaaaac aaagatattt ctacaaattt
     7561 acaaacaatt tcaagagtgg ataatttgtt gaaaaatgct tgctgacaaa gaatttggtt
     7621 tcgacctgca tagatttatt ttatacgaac gtgtgaaaac aaaatcacac ttacgcatgc
     7681 aataaaagtt tataacagac tgataataaa caaaaaagcc ctctgaaatc cagagagctt
     7741 tgtaaaaatg aaatatcaga tttttctgtt ttatgtgatt cttaaccctt gtaaaaaggc
     7801 gttttcacaa ccgcagcttt cagcagttta tcgcggactt tgatataaat ctcagaaccg
     7861 gttttggcca tttcgagcgg aacataaccc atgccaacac caattttcac gctcgggccc
     7921 atggttccgg aagtcacatg gccaaccaca gtgccatctg ctttgcagat ttcgtactca
     7981 tgacgcggga ttcctttgtc gatcatttca aaaccaacga gacgacgttt ggcaccgcca
     8041 tttttcagag caagattata atctttgtcg atgaaatttt ttgtatccgt aaacttggtg
     8101 atccaaccca atccagcttc aatgggagcg gtagtgtcgt cgatatcatt gccatagaga
     8161 cagaatccca tttcgaggcg cagggtgtcg cgggcagcca aaccgatggg tttgatgcca
     8221 aattcagcgc cggcttcaaa aacagctttg aaaattttgg ctgcatcagc atttttcatg
     8281 taaatttcgc atccaccgct gccggtataa ccggttgtgg caaaaataac atctttaaca
     8341 ccggcaaatt caatcacttt cacggtatag tattccatgt cggtaaccgt tgtagccgtg
     8401 agtttctgca tagctttaag cgcaaggggt ccctgtacgg ccaactgagc ataatcatca
     8461 gattcatttc tgaaatctac gcccactttc agtccgaatt tttcggcatg cggagccatc
     8521 cagttccaat ctttatcgat attggcagca ttgactacga gcagataatt gtcggctgca
     8581 atgcgataaa ccagcaaatc atcaacgatt ccaccctttc catttggcag gcaggaatac
     8641 tgaactttgc catcaacaag cactgaagcg tcgttgctgg tcatatgctg aatgaaatcg
     8701 agggctttcg gtccctgaac ccagaactcg cccatatgag aaacatcaaa aacgccaaca
     8761 cctttgcgaa cagtttcgtg ttcaacacta acaccttcga attgcaaagg catgtagaag
     8821 ccggcgaatt caaccatttt ggcgcccatt tcctcgtgaa attttgtgaa tgcagttgtt
     8881 ttcatttcta ttgaattttt cagaattgtt gcaaaggtaa atgaattatt aaatccatga
     8941 tcaaaacttg atgaaaatta caacagacac attgaattga ttagcgtaac aacaacgtag
     9001 cacagccgat aatgaaaaaa gcgcccattc aggcgctttt tatgcttcta caccaataaa
     9061 cccaaaaacc tatgaaaata attttgatgc agcagtatta ttttttaaaa ccccggttac
     9121 gcaacaacgc atcaaccaat ggctcacggc cacggaatgc aacatacatt tccattggat
     9181 ctcgtttacc gcccggagcc aacacttcgt gacggaattt tgcagccagt tccttattaa
     9241 aaatatcacc tgactcttca aaagcttcga atgcgtcact atcaagcact tcactccaga
     9301 cataactata atatccggca gaataaccac cggcgaaaat atgattgaaa taggtggagc
     9361 gatatctcgg aagaatttct ttcatcaatc caagtctgtc catcgatgct ttttcaaatg
     9421 caagaatatc aagattgctg gcattttcaa gtgtatgata atccatatca agaacagccg
     9481 ccgcaataaa ttcaagcgtg gcaaaaccct gattgaaatt accgcttttt tcgattttgg
     9541 caatcagttc atccggaatc acttctcctg ttttgtagtg tttggcatac aatttcagca
     9601 tttcgggatg ccgagcccaa tgctccatta cctgcgaagg taattcaaca aaatcgcgcg
     9661 gtacataacc cgaaagacgc ttgtatttga cattgctgaa aaaaccatga atagcatggc
     9721 caaattcgtg gaaaaaagtc aatgtctcat ccagattcaa caaagccggt gaattggctg
     9781 taggtgcagt aaagttcatt acaatactaa ccaccggagt aatattctgt ccgtttctta
     9841 catactgatc acggtaggat gtacaccagg cacccccacg tttcgaagca cgcggatgaa
     9901 agtccatata caaaacggcc agatgctgat tgttggtgtc caaaacttca taaacagtta
     9961 catctttgtg atacaccggt aaattgtcaa tttttttaaa tgaaatatca tacaatttat
    10021 tactgagtag aaaaattcct tcacggacat tgtcgagact aaaatatgga cgcagcattt
    10081 cttcgtcaag atcgtatttt tctttacgta ttttttcggc ataaaaccac caatcccatg
    10141 gctccagcag gaatttgcca ccttccttat caatcatttt ctgcatttca gcagcttcgg
    10201 cttttccaac aggaagcgcg gcatcccaca cagtctgaag aagatcataa accttttccg
    10261 gagtttttgc catattacgg tcgaggataa acgaggcatg attgagataa ccaagcaatt
    10321 tggcgcgctc cagacgcaat gatacaatct gggtaatatt ttgcttattg tcgtttttat
    10381 tgttattgtc accacgcatg aaatatccct tgtagatttt ttcacgaagg tcgcgacgtt
    10441 cagaatactg aagaaaaggg atcagacttg gtttgtcaac agtaaacaac cattttccct
    10501 ccattttatt ttgctttgct gcttccgaag cggcatcaac aacactttgc ggaagaccag
    10561 caagatcttc attcttttca atcaccattt gaaaagcatt gttatccgcc atcacattat
    10621 cgccaaactg caactcaagc aaagacaatt tctcattgat ttcgcgtaaa cgctccttgc
    10681 cttttttgtc aagatttgca ccgcttctta caaaatcgag gtaaatgatt tctgtgagtt
    10741 ttttctcttc aggattcagg ccactgcgat tgttataaac tgtttgaatg cgtttgaaca
    10801 acttttcatt aagcatgatg tcgtcgctgt gtttcgacat tttcggggcc atttcctgag
    10861 ccagattttg catcgaatca ttggtgttgg cttccaactg attgtaaaac acagagctca
    10921 cacgagaaag catctgacca ctggcgtcgt attttgcaat agtattttcg aaagtagctt
    10981 cgtcttggct gtttgcaata gaatctatct caagcatctg ctgcttcatt ccctcttcga
    11041 gcgccggctg ataatgttcg aatttgattt tatcaaacgg aggtactcca aactgggtgt
    11101 tgaattccga aaagaaggga ttgtccttat tctttttatc ggcaccacca catcctgcga
    11161 tgagtatcat gactacataa ggcattgctt tcaatgagaa aatgtttttc atgtcttatt
    11221 tttctgcaaa gctacaatct taataaatat caaaacatct gataattaat aaaatatact
    11281 gacacttggt tttcacaaac cgatggcaga taaaaatatg tacgcaatgg aaattattcg
    11341 cttaaagtca aataattatg gactataatg ctgcaaagca tcacagatca gggttttgga
    11401 gggctgcagg ggtcgcaggg aaaagaatac agcaacttat aaccagacca cgaaccatct
    11461 ccaacggtta tttttccata tattctctca taatatttct gacaaccggg attgggaggc
    11521 catttgctgc cttcggtata aaccagaaaa taatcataac taccttgatg caaaacaacg
    11581 gtaaaacatc cattctcatc ggttgaaaca ataacaaaat cggatttgtc gtcaacgttc
    11641 acaaatttaa ttttctgtga aaccaataaa tgctcctgat tgaactcctt ttcaatgtca
    11701 ggtgtaggcc tggctcctcc gcaataacgc agcttataaa aagcagtgcc actggcaatc
    11761 tcttcctttt tccaattccg accatcctga ttcgaagcat tattatcaac aatcaatgct
    11821 tcagccgggt caacggccac cgcagtatcc cgaaattgct cttctgcaac aatactgtcc
    11881 gatgtttccc tgacaacttc ttttttgtgt ttgcagaaat tcattgaccc cataatgatg
    11941 gcaagcaaaa caatgtgctg gattttcatt ttctgagaat tgtaaaacat acaaacttac
    12001 acaatttttt caggcagcaa caaatacaat taactgttta aataaaagaa cggtcaatcc
    12061 gcgaaaaaaa tgcattagca ctattgctgt tttaataatt gttttacttt tgcaaaaaac
    12121 ttaaccggga tgtagcaatc cataaataaa accaggaaag gaggcggcct atgattgaac
    12181 agatcaggct tgacaaagta aagtggttac atattcttta tccatctgat gaagattaca
    12241 atctcctgat aaacgaatac cattttcacc ccctggatat tgaggactgc aaaacacggg
    12301 cgcagcgatc gaaaattgac atctacgatg attactattt tctgattctg catttcccct
    12361 atttcgataa aatcaaccgc tttatcaaag tcaaggaaat aaaaattttc tggggcgaag
    12421 attttatcat taccattgga agtgcccact gggttatcag ggacttattt gaggaactcc
    12481 gccggcaaat aaatgatggt cagattgatg aagatctgtc atcatcagat aaattactgt
    12541 atcgcattct ggaatcgctg atgaaagaaa cgcaggtcat tcttcaacga accgatacag
    12601 aaattgattt gatcaataag gaaatgttca gccgcaatgc agataagatc attgaaaaaa
    12661 tatcaattac aagaaaaaat gtaatcctgc tcaatacaac tttcaaaccg cagctacggc
    12721 tgttccacaa atttgaaagt ggcgaagtca aaggatttgc cgaagacatg gaagattact
    12781 ggggaaatat tctcgactac tatcagaaaa actgggatat gattgaagat gatggcgaac
    12841 tgctcgaagg actctcgaaa acatttgact cgatgcaggc caatcgcacc aacgaaatca
    12901 tgaaaatgct cacattcatc agcacaacat tactgccgat gacatttatc accggattat
    12961 acggaatgaa tgtggcattg ccgtttggcg agcatccctt tgcgttttca atgataatag
    13021 gcatcatggc tctgatcgtt atttttctga ttctctattt caaacgaaaa cgctggatgt
    13081 aatcaaagga atcaatgaga ggaaataaag gtgtttcact gctattcatg ttaattctgg
    13141 tgctcacctg gggttcttcg ttcattttaa tgaaaaaagc gctggtgtat ttcccaagca
    13201 acattgtcgc ggcataccga atgacagctg ctttttctat catgctaatt cctgcattga
    13261 tatacatttt caaaatgcca tttaaaactt ttcttcttct aacgttgtca gggattctgg
    13321 caaatggtct tcctgccttt ctgtttgctt atgctcaaac cggcattgaa agctatgttg
    13381 caggtatcct gaatagtacc acgacgctgt ttacattgat cataggaatc atttttttta
    13441 gctacaaagc cagattgctt agtattgccg gcgttgttat cggatttgcc ggcatcgttg
    13501 gattgctggc ctttgcaggg gataaaacac tctcgttcaa tttttcctat ggcatatata
    13561 ttctcattgc aacaacatcc tatgcaatca acatcaattt tatcaaacgc tggctcagta
    13621 atgtacccac cctttcaatg gtttcggtag tttttttcat caccggattg ttttctacta
    13681 tatatctctt tggatacacc gattttattc agataacaaa aaccacagca ggtagcggat
    13741 ttggaattct gtacatgagc attttgggaa ttgtgggatc agcaatggct ttgctgatgt
    13801 attatcattt aataaaaata gcagatattc tttttgtggc atcggtcact tatctgatgc
    13861 ccgtggtgtc aaccctctgg ggactaggcg acggcgaagt tctggggctg gcacacatcg
    13921 catttattct gctcattctt attggcgttt tcatggtgaa ttatcatgat cttatgggga
    13981 aaatcaaaca gcgatacaaa taaaaaccga accgggatac aatcacaaat acaattgcaa
    14041 tcccagtccg gttattcaat tatgaataaa aatctatttg cccgacttgg tgttgtttgt
    14101 atccaacact tctttgcgcc gtttctcaat aaccatatac tgaaattcag cagtggtttc
    14161 aacatcggat ttcacgacaa attgtttgct ttcctgattg gcaatataca acacagccgg
    14221 cttgccgaca ggagtgagaa caatataata gtttgccgga tcaatttctt cattgagtga
    14281 aatctgacat tgaccgttat taagaacagc cgttccggtt tcaagaattc tgattttttc
    14341 aacatgtttt gtttgctgtg ctgacatagc aaggcaaata acaaagaaaa cagagaccaa
    14401 taatatcttt ttcataccaa ttcaatttca gtgattaatt tctcaaaaca aagacctgaa
    14461 gaacactttc ggctgcctgg gtagcatcgc ctccgataaa aatggattct gacacaccaa
    14521 gcgttgctcg ggcacgaaca tcaaaagtat aatccccagc aacagtaaca ttatatcgtg
    14581 caatggccga ataattctgc catgaaagtt ctgcataatc ccagtcaatg gccgtacgga
    14641 caaaaccacc taccacagcc attgttccgt ttatatgcaa agccacatca acgatattgt
    14701 aatcagcgcc atccatatac atgtttccac caaaccagat caaaacgcgg tcgccgggat
    14761 tcaatccgtt aacagtgatg gattcacccg gaataactgt aaatgtggag ctggtaattt
    14821 caagagctgc cgtactttcc actgtgtaaa tattgtctgg agtcacagca ctgatccatg
    14881 taggagcaac ggctgccccg tttgatttca gtacttttcc ggaagttccg ggatcattgc
    14941 ccggcatcaa agcccctgag aagcgaatat tgccggttac atccagtttt tcggtgggag
    15001 cggctgaagc tatacccaga ttgttggtaa tatacacatt acccccggca aaatatccgg
    15061 cccagttcgt tgtaccaccg gatgcactgg cataaaggcc atagactgat gatccattat
    15121 ttgcggtgga ataaatgccg taattgacac caccattatc accacgactg actatattgt
    15181 atataccata tcaggcgtgt ccggttataa gtcaaacaaa tccagttgat taggatcgcg
    15241 tgt
//
LOCUS       DAOPKE010000064        14980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4184_length_14980_cov_13.857487, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000064 DAOPKE010000000
VERSION     DAOPKE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_14980_cov_13.857487"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PKY63_08830"
     CDS             <1..1330
                     /locus_tag="PKY63_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00756.1"
                     /translation="SVNTSYPVSVDVVASPAGAVCSGIPVTFTATAVNGGTAPVYQWY
                     VNGIATGSNLNTYESSTLSTGDVVTCEVISDMTCAMNNPATSAPVTMIVNTTLPVSIS
                     IDASPAASVCEGTEITFTATGVNGGLNPIYAWTVNGSAVGTNSPVFSSLTLTNGDIVS
                     CTMTSDEVCTSNNPAVSNSIDIVIAPYTAGGVMSSMETEVCEGESTGTIILSGNVGDI
                     LYWQRRIDGGTWTNIPGTAGFGTYSEITTAAGVWEYRAVVQSGACNVDTSALIGINVI
                     ATPVASFTYNNLDPTFEFINTSMNASSYSWSFGDGGTSTAMNPVHTYTSDNTFTVTLT
                     AYNGACQNEYTTTIDVLSVSIFELGDISLQMFPNPSDGEFDITLSGSNTGSLQMEIYD
                     VTGRLIRTEDLSSMNGGSVFHVNLGEVNAGLYNVRFIYNEQVVTKVMVVE"
     gene            1407..2105
                     /locus_tag="PKY63_08835"
     CDS             1407..2105
                     /locus_tag="PKY63_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00757.1"
                     /translation="MKKWILLSLVFLAACSTEITINTDSFNLKGKVKSITDIQSQGIY
                     DKSWNVYPDTAVSVSFISIRYFDEDGKWVSAKMELTNGTVYSTTEVLYDDDGNYAGTK
                     DLDANGSLFRETSVTEISENKIVMVTKSGSGKDVSENVSEYEDGLVMKQTTNWSDGTL
                     KITYEYKRDSEGNEEEISIHSVNTNSNPPVTDNVLKVKILKTDEYGNWIRQAYYSSTD
                     NTCTIIDRKIEYYE"
     gene            complement(2291..2986)
                     /locus_tag="PKY63_08840"
     CDS             complement(2291..2986)
                     /locus_tag="PKY63_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF00758.1"
                     /translation="MIDINKHKFFMMQILKDIYNDIELSSALGFKGGTALLFFHDLPR
                     FSVDLDFNLLDLSKEDLVYQKLRALLLLHGGIFDEAKKHFSTVIVLNYGMGERKLKVE
                     ISKQNFGDRYEIKNFLGVWMKVMTEPDMFAHKLCALTDRTATANRDIFDCWFFLNRRA
                     PVNRTIVESRMHVSLENHIQTCIELLEKKSDKGFLNGLGELMDDETKKFVKTKLRTET
                     ISLLKFYQSFPIE"
     gene            complement(2979..3542)
                     /locus_tag="PKY63_08845"
     CDS             complement(2979..3542)
                     /locus_tag="PKY63_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00759.1"
                     /translation="MDIIATLYGNPKTVFTLPETALLLNEQNFVSLSKRLNYYVKTGR
                     LLNLRKGIYAQPPYNREELACCIFAPAYISLEYILQKAGVVFQFDSRITLVSYLSRTI
                     EVDRQMYSFRKIKNQTLINTKGVLRLENGVNMATPERAFLDLLYLNDDYFFDNLKPLD
                     RQLIQDLLPAYNSKALIKKVQKQFKND"
     gene            3811..4308
                     /locus_tag="PKY63_08850"
     CDS             3811..4308
                     /locus_tag="PKY63_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00760.1"
                     /translation="MYLNKETSMFFRLIPLIIILCVGCRTTSKTDQINVIGEDNKDVV
                     ALSYLIRDYMRQTDNTNFTLSDIIQFDTLGRVTNNFSGLEVANWSNVWLGGYAVYFKF
                     SDNRNKDSIKLTESERMPWKVKTKEKIGKNDEQLSKNFDGEIHFYFPERRYNIAEIIL
                     RKHEN"
     gene            4618..4911
                     /locus_tag="PKY63_08855"
     CDS             4618..4911
                     /locus_tag="PKY63_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00761.1"
                     /translation="MMLFALKCFIFAERTKDMNIQARKLVLIEEFLRISDESFIAKLE
                     SFIKQEKKVSHERNLKPMSLNEFHEMIGQAKRDSEEGRVISHQELKKKVKSWK"
     gene            4902..5210
                     /locus_tag="PKY63_08860"
     CDS             4902..5210
                     /locus_tag="PKY63_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF00762.1"
                     /translation="MEIRIEWSELSERQLKDIFDYYAFVASPGIAKKIVSRILERVSV
                     LETNPLVGPKEELLVEYPEDFRYLVESNFKIIYWKNEDFITIASVFDCRQNPEKIKKL
                     "
     gene            5566..5865
                     /locus_tag="PKY63_08865"
     CDS             5566..5865
                     /locus_tag="PKY63_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016213099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF00763.1"
                     /translation="MSTIFGAKIRTFREEKRMPQRQIAAALEIDTATYCKIEKGDRRA
                     KREQVVLLSQILCCKCDELLKVWLADQVYEMVKNEKEAKEVLSLVQESIVAYEKR"
     gene            5852..7237
                     /gene="dcm"
                     /locus_tag="PKY63_08870"
     CDS             5852..7237
                     /gene="dcm"
                     /locus_tag="PKY63_08870"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPF00764.1"
                     /translation="MKKDNQTPTRKIKFIDLFAGIGGIRCGFEQAAKSYGFETECVFT
                     SEIKPYAVEVLKQNYPNEVINGDITKIPASEIPDFDFLFGGFPCQSFSAAGKRLGFLD
                     TRGTLFFEIERILRDKKPFGFILENVEGLINHDKVDKKANVGRTLSTILARLDELGYN
                     VEWKLLNSKNFGLAQERKRVYIVGTKTEKPSLENFVEHYSKLKSVIEEGQPIIKTKFT
                     DLLLTHFSVEQLYGKSIKDKRGGKNNIHSWDIEFKGPVSEEQKKILNTLFKERRKKKW
                     AQEFGIDWMDGMPLTLEQISSFFKHDNLKGILEDLVKKGYLKKEHPKRLVKETNLLGE
                     IKTIRKQDRSLPLGYNIVSGKLSFEVNKILCPNDIAPTLVAMDMKKLFVVDNGGLRTL
                     TLKEGLKLFGFPENYKFNISTDEGYDLLGNTVTVPIVKAVSERLLTIWKKQKTRKHET
                     NSTRSFQQVNA"
     gene            7194..7928
                     /locus_tag="PKY63_08875"
     CDS             7194..7928
                     /locus_tag="PKY63_08875"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090305 - nucleic acid phosphodiester bond
                     hydrolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HPF00765.1"
                     /translation="MKLTAQEVFNKLMLEDKILEVKGQIKFYLGNVSIVVRQRDVIGN
                     IMQEWLEGWLNQNQVLHKINTNTQMPPDFYLNPDDLTSELLEVKAFNYDASPGFDIAD
                     FNAYQSEIIEQPFMLHTKYLIFGYQMQDDGYVVIKKLWLKNVWEICRRMEKWPLNLQI
                     KDKVVHKIRPCTWYSQGRSVKFKPFESLEDFISAMEQTVYQNPKTREKSGEWLKVFLK
                     SYENKYNTKLKVPRWDDIEDNYINKK"
     gene            complement(8128..8814)
                     /locus_tag="PKY63_08880"
     CDS             complement(8128..8814)
                     /locus_tag="PKY63_08880"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF00766.1"
                     /translation="MENKIINLRMVSEVASALHELNEKMVFVGGAVISVYTDDPAADE
                     IRPTSDIDMTIKLATYTDWVNLNKRLLELKFAPNPAGHALCSFLYNQMEIDIMPSETG
                     ALGDSNRWYKPGFESLQKIEVHGQIIHVLSAPYFIATKLEAFHSRGTDYRTSHDFEDI
                     IYIIDNRTTIVKEVSDADDAVKKYLKDEFVKILSNENFPEIISVHVHPLMREERMSLI
                     IEKLQKCALS"
     gene            complement(8804..9322)
                     /locus_tag="PKY63_08885"
     CDS             complement(8804..9322)
                     /locus_tag="PKY63_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00767.1"
                     /translation="MKSSNNQMKPQDVVILLKIIAIGSDHWQQKPLADSLKMSQSEIS
                     QSASRMQYAGLLLGKKVMRLAFFDFLKYGLSYVFPQKPGPVVRGIPTAHSAPPLSNKI
                     LSDEQYVWPFASGKVRGHSIAPLYSSVPDAALNDSALYELLALVDAVRVGRTREKELA
                     LKELEKRILHGK"
     gene            complement(9503..14185)
                     /locus_tag="PKY63_08890"
     CDS             complement(9503..14185)
                     /locus_tag="PKY63_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF00768.1"
                     /translation="MNNTIIIRNARANNLKNISLEIPHGKLVVACGVSGSGKSSLIYD
                     VIGKEGQRLFYEHFLPGSYHQNIKLGRPEADQIEGLPAVLMLNQQGNASSPRSTVGTI
                     TGLWDLLRLLFARYAHSDSVTESNRSLFSFNSPAGQCPQCKGLGVEDHIDPQLLIGDD
                     TKTLREGASVLTTPNGYIIYSQVTMEELNKVCVAEGFSVDIPWKDLTDAQKNIVLNGS
                     EKIKILFGKHTLESRLKWSGITAKPREEGFYKGIVPVMEEILKRDRNPNILRFARSFT
                     CQSCSGTRLRNEAMSFEWNNKNIDDFHRMSIEEIHRTFTELTSENEGLMQISEQILKN
                     TTTLLELGAGYLQLNRESSTLSTGELNRLRLAAYTTSGIRNILYILDEPGAGLHDSEQ
                     QSMMKVLRRLVDHGNSVIVVDHHEKNIAAADYIIEIGRGAGNDGGEVLFSGNASQYFT
                     QNISDSPTQKWLHNIPELKSEKLSNPAFEIHQANKNNLKNISVSFVRNAFNVLCGVSG
                     SGKTSLLSVVHDQATQAGFSKIIHIDADPFGRSPRSNPATYTGLSDILRDLYASTASA
                     KENKLTKTHFSLAVPGGRCEDCMGAGLKQIGMHFLGNVEIPCETCGGMRFSETVLHIQ
                     IREKNMHDVLELSVAEAHEFFSAEPKVLKYTRPMLDLGLGYIKLGQSSTTLSGGEAQR
                     VKLASELVRGTSGSVLFLLDEPATGLHPHDVNNLISALRELTQKGHTVIAAEHDLRMI
                     AQADHICELGPGSGTHGGNVVFSGVPAELISNKNSKTAQALSDFAEPKPASEVSLQNQ
                     KTAFSDITLKQVHTNNLDIAEIQFHENVVNAVCGPSGSGKSSLVFDTLYAASQNALMD
                     GLSFRIRSQLKKAGNAALDEYTGLMPTIALEKKSASGNPRSTVGTYTGLYDLYRLLYS
                     RFSKNGETTCPHYSNAFSFNNEAGACPDCHGLGFITQCDAEKLVSDPSKPLIQGAIDG
                     SKPGKFYGEPDGQYVAALLAVGKKYGIDFSVPYSELSADARSIAMHGCGDESFDVEWN
                     YKRGNVSGVHKMKSKWPGFCGHVEEEYHRVHNDHRAEQILPVMSDMTCPSCGGYRIHH
                     ERLQYRIDNRHIGETTDLCASEAISWFQHIQPGTGSENIVAEILQRLNYLIESGIGYI
                     ALSRMTATLSGGEFQRLRMSALLQSQLSGVMIVMDEPSFGLSKSDCSRISQLLKNIVQ
                     RGNTLVMTDHHPEMIQLADRIKVLGPGAGKQGGRLVKEVSSTEYISEIEQTYLKVTPL
                     HSTSGEALQVTGASANNLKNIDADFSGGTLNVITGNSGSGKTSLLQYVMYDSHMAGRP
                     VKCRSISGFENFDSVVFIQQDVPSGSAASIPATWLGLYDTLKNIFAAEAARLKLPLKA
                     THFSVFSKEGRCPECGGTGVIKTSMDFRSDSETVCESCNGARFRSDILNVVVDGFSIS
                     DVLNMSISEAAEFVQKNTTAAKAASFLTIADLCTRCGVDYISPGQNLSTLSTGELQRL
                     KLIQGIASAKGKTLFLLDEPSGGLHPTDTLRLLHLFEHLVKQGHTIICATHDEMIRRA
                     AANNLILR"
     gene            complement(14182..14334)
                     /locus_tag="PKY63_08895"
     CDS             complement(14182..14334)
                     /locus_tag="PKY63_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00769.1"
                     /translation="MNSSGYYYFTPEFTPQDAEEYLVELRVKRDKSQLFICLRRFITH
                     FLIFAS"
BASE COUNT         4365 a         3016 c         3307 g         4292 t
ORIGIN      
        1 gtcggtcaat acttcttatc cggtcagtgt tgatgtggta gcatctcctg ctggtgcagt
       61 ttgttcgggt attccggtaa cttttacagc tactgctgtt aatggtggca cagctcctgt
      121 ttatcagtgg tatgtaaatg gtatcgctac cggatcaaat cttaacacgt atgaaagttc
      181 aacgctttca actggcgatg ttgttacctg cgaggtgatt tctgatatga cttgtgctat
      241 gaataatcct gcaacttcgg cacctgttac aatgattgtc aatacaactt tgcctgttag
      301 tataagcatt gatgcttctc cggcagcttc tgtctgcgag ggtactgaaa ttactttcac
      361 agcaacggga gttaatggtg gtttaaatcc gatatatgca tggactgtaa acggatctgc
      421 agtcggaaca aacagccctg ttttctcttc tttaacattg acaaatggtg acattgttag
      481 ttgtaccatg acttcggacg aagtttgcac aagcaataat ccggcagtgt ctaacagcat
      541 cgatattgtg attgctcctt atactgcagg cggagtgatg agtagtatgg aaactgaagt
      601 gtgtgaaggt gaatcaacag gaaccattat tctgagtgga aatgtcggtg atattcttta
      661 ctggcagcgt agaatcgatg gcggcacatg gaccaatatt ccgggaacag caggatttgg
      721 tacatattct gaaatcacaa cagctgcagg tgtttgggaa tacagagccg tagttcagag
      781 cggagcatgc aatgttgata cttctgcatt gattggtatc aatgtgattg caacacctgt
      841 tgcctcattt acctacaata atctggatcc aacatttgaa ttcatcaaca catccatgaa
      901 tgcttcatca tatagctggt cgttcggcga tggtggtact tcaacagcga tgaatccggt
      961 tcatacttat acttctgaca acactttcac ggttactttg acggcttata atggcgcttg
     1021 tcagaatgag tacactacca cgattgatgt tttgtcggta tcaattttcg aactgggtga
     1081 tatcagtctg cagatgttcc cgaatccttc ggacggagag tttgatatta cgctttccgg
     1141 atcgaataca ggatcactcc agatggaaat ctatgatgta acaggacgac tcatccgcac
     1201 tgaagacttg tcttcgatga atggcggcag tgtgttccat gtaaatcttg gtgaagtgaa
     1261 tgctggattg tataatgtcc ggtttattta caacgaacag gttgttacca aggtgatggt
     1321 tgttgaatag atttttaata atcagattaa aaagggacag ctcagctgtc cctttttttg
     1381 taatattgcg ttgaaaaatt aacaccatga aaaaatggat tcttctttcg cttgtttttt
     1441 tggctgcctg cagcactgaa ataaccatta ataccgactc ttttaatctg aaaggaaaag
     1501 tgaaatcaat taccgatatt cagagtcagg gcatttatga taagagctgg aatgtttatc
     1561 cggatacagc tgtatctgtt tcgtttatca gcatccgcta ttttgatgaa gatggaaaat
     1621 gggtgagtgc aaaaatggaa ttgaccaatg gaacagttta ttcaaccact gaagttttgt
     1681 acgatgatga tggaaattat gcaggaacaa aagatctcga cgccaatgga agtctgttca
     1741 gggaaaccag tgttacggag atttctgaga ataaaatagt gatggtaacc aagtccggtt
     1801 caggtaaaga tgtttcagag aatgtgagtg aatatgaaga tgggctggtg atgaagcaaa
     1861 ctactaactg gtccgatgga acattgaaaa ttacttatga atataaacgc gacagcgaag
     1921 gcaatgaaga agaaatttcc atacattctg tcaatacaaa ttcaaatccg cctgtaactg
     1981 ataatgtgct taaagtgaaa attctgaaaa ctgatgaata tggcaactgg atccgccagg
     2041 cctattacag cagcaccgac aacacctgca ccatcattga tcggaagatt gaatattatg
     2101 aataatgatc agataggtcg aggcttgagg tcaaggtgtt ttgtcatagc tgactgctcc
     2161 agctgtaagc attcagaaag gttaagcgcc gatggagtca tgtgaaggca taaaaaagct
     2221 acgaagtacg agactgttca agcggaacaa aatgttgggt gccagtcaag ctttctcaca
     2281 aagtattctt tcactcaatc ggaaacgact gatagaattt taacagcgaa atggtttcgg
     2341 ttcgcagttt tgttttgaca aactttttgg tttcatcatc catcagttcg ccaagtccgt
     2401 tgagaaaacc tttatcactt tttttctcaa gaagctcaat gcatgtctga atgtggtttt
     2461 ccagcgacac atgcattctt gattcaacaa ttgtacgatt gaccggcgcg cgtcggttca
     2521 gaaaaaacca gcagtcaaaa atatcgcggt tggctgtggc agtgcggtcg gtcaacgcac
     2581 aaagcttgtg tgcaaacatg tcgggttcag tcattacttt catccagaca cccaaaaaat
     2641 tttttatttc ataccgatcg ccgaaattct gtttcgatat ttcaaccttt agtttccttt
     2701 cgcccatgcc gtaattcaaa acgatgacgg tgctgaaatg cttctttgct tcatcgaaaa
     2761 taccaccgtg caaaaggagt aaggcccgga gtttctgata taccaggtct tcttttgata
     2821 agtccagtaa gttaaaatcc agatcaacgg aaaaccgcgg caggtcatga aaaaacagca
     2881 atgccgttcc ccctttaaaa cccaatgcag atgacaattc tatatcgttg tagatgtctt
     2941 tcagaatctg catcatgaaa aacttatgct tgtttatatc aatcattttt gaattgtttt
     3001 tgaacttttt ttattaatgc ttttgaatta taggcgggca gtaaatcctg tatcaattgc
     3061 ctgtcaagtg gcttcagatt gtcgaaaaag taatcgtcgt tcagataaag caaatcaaga
     3121 aatgcccgtt ccggcgtggc catgttaact ccgttttcca atctcaaaac gccctttgta
     3181 ttgatcaggg tctggttttt aatctttctg aatgaataca tctgacgatc cacttcaatg
     3241 gtacgcgaaa gataacttac cagcgtaatc cggctgtcga actgaaacac aacgccggcc
     3301 ttctgcaaaa tatattcaag cgaaatgtac gccggcgcaa atatgcaaca ggccagttct
     3361 tcgcggttgt atgggggttg cgcataaatt cctttgcgga gattgagcaa tcgtcctgtt
     3421 tttacataat agttcaatcg cttgctcagc gaaacaaagt tttgctcgtt cagcaacaac
     3481 gcggtttcgg gtaaagtaaa taccgttttt ggattgccat aaagtgtcgc aataatgtcc
     3541 ataatgatga aatatgaacc aaagatacga aatattcgta ttattggttc acaaaagtaa
     3601 tttaaatgtt taaaaacgag ttgtgtcaga ccaattgatt ctcccacgca tggtatcatc
     3661 ctgatagtaa tattctgaag aatcgactgc aaaaaataaa tctccttttg aagatttata
     3721 actcaaaata aattatatct ttggaaataa tatgcacctt ttggaggtgt atatgcaaac
     3781 gatttagacg gcactgtaat acgcaagcct atgtatttaa ataaagagac atctatgttt
     3841 tttcgattga ttcctttgat aataatttta tgtgttggtt gcagaactac ttcaaagact
     3901 gaccaaataa acgtaattgg tgaagacaac aaagacgtgg tagcgctttc atatctaata
     3961 agagactata tgcgtcagac tgacaacaca aacttcacac tttcagacat tatacaattt
     4021 gatacattag gtcgagtcac aaataatttt tccggattgg aggttgccaa ttggtcaaat
     4081 gtgtggctag gtggttatgc tgtatatttc aaattctcgg acaatagaaa caaagattca
     4141 attaaactca cggagtcgga aagaatgccc tggaaggtaa aaacgaaaga gaaaatcggg
     4201 aagaatgatg aacagctttc caaaaacttt gatggagaaa tacattttta ttttcctgaa
     4261 agacgttaca acattgcaga aataatattg agaaaacatg aaaactaaaa cataatgaaa
     4321 taatgtacag cgattgccta acacgggatt tgcgccagag ggaggacagc gcgaatagaa
     4381 acatttcagc aattaataaa cgttggtgct ggcatacagt ttacattttc gaaagcccgc
     4441 caacgcaaag tccgaaaact ctcagcaggc catatttcca aattcctttt ttcaccatac
     4501 caagtcgctt cgaataacaa caatcaaaat ctcattcatt atgatttttt tgtatttttg
     4561 tgggggaacg gtgggtaaaa tgatggggcg ttagggccat gctgaaaaat tgccgatatg
     4621 atgcttttcg ccttgaaatg ttttatattt gcagaaagaa ctaaagacat gaacattcaa
     4681 gcaagaaagt tggttttaat tgaggaattc cttcgaataa gcgatgaaag ctttatcgct
     4741 aaacttgaat cgtttatcaa gcaagaaaag aaagtctctc acgaaaggaa cttaaaacct
     4801 atgtccttaa acgaattcca tgaaatgatt ggccaggcga aacgtgatag cgaagaaggt
     4861 cgggttatct ctcaccaaga gctgaagaag aaagtcaaat catggaaata aggattgaat
     4921 ggtctgagtt gtcggaaaga caactcaaag acatatttga ttactacgcc tttgtggcaa
     4981 gtccaggaat tgcaaaaaaa atagtcagta gaatcctgga aagagtatct gttttagaaa
     5041 ctaacccctt agttggcccg aaagaagaac ttttagttga atatcccgaa gactttagat
     5101 acctggttga aagcaacttt aagattatct attggaaaaa tgaagatttt atcactattg
     5161 catctgtctt tgattgtaga cagaatcctg agaaaataaa gaaactatag ccaggcacca
     5221 ttaacgaaag cttcatatga ctgaggcgtg tcaggccact ggcggaaaca aagccaaaca
     5281 acgcttctgc cttcaaataa atgaagcatt attgcgcgga tttgcaagca gccttaatcc
     5341 agtccacctg gccggttgga caggattcat tttttcacca ttcccaagtc gcttcaatga
     5401 acaacaaaca aactttttat tcattatgat ttttttgtat ttttgtggga ggaggatggg
     5461 tgaaacgatg agacgttggc agcaatccaa aaaacaggat gtgaaaaaaa ttgaaaactg
     5521 aaagaaaaaa gaattatatt tgccaaataa tgtcaagaca accccatgtc aacaatcttt
     5581 ggtgctaaaa tcaggacttt tcgagaggag aaacgaatgc ctcaaagaca aattgcagca
     5641 gcattagaaa tcgatactgc tacatattgt aagattgaaa aaggagacag acgcgcaaaa
     5701 agagaacagg ttgttttgct ttctcaaata ctttgttgca aatgcgatga attactcaaa
     5761 gtttggttag cagaccaagt gtatgaaatg gttaagaatg agaaagaagc aaaagaagtg
     5821 ttaagtttag tacaagaaag tatagttgcg tatgaaaaaa gataatcaaa cacccactcg
     5881 aaaaatcaaa ttcatagatt tgtttgcagg tatcggcgga atcaggtgtg gatttgaaca
     5941 ggcggcaaaa tcatatggtt ttgaaactga gtgtgtcttt acttcagaaa ttaaacccta
     6001 tgctgttgaa gtgcttaaac aaaattatcc taatgaggta atcaatggag atataacaaa
     6061 aattccagct tcggaaatac cagattttga ttttttattt ggaggatttc cttgtcaatc
     6121 attttcagca gcgggaaaac gattaggatt tttagataca agaggaactt tgttttttga
     6181 aattgagcgc attttgagag ataaaaaacc atttggattt atattagaaa atgtcgaggg
     6241 tttaataaat cacgataaag ttgacaaaaa agccaacgtt ggtcgcacac tgtcaactat
     6301 tcttgcacgt ttagatgaac ttggatataa tgtggaatgg aagttattaa attcaaagaa
     6361 ttttggatta gctcaagaac gaaagcgtgt atatatcgtt ggaacaaaaa cagaaaagcc
     6421 ttcattagaa aactttgtgg aacactattc aaaactaaaa tctgtaattg aagaagggca
     6481 accaattatt aaaacaaaat ttacagactt gcttctaaca cattttagcg tggaacaact
     6541 atatggtaaa tcgataaaag acaagcgtgg aggaaaaaac aatattcata gttgggatat
     6601 tgaatttaaa ggacctgtat cagaggaaca aaagaaaata ctaaatactc tttttaaaga
     6661 aagaagaaag aaaaagtggg ctcaggagtt tggtattgat tggatggatg gtatgccatt
     6721 aactttagaa caaatatcaa gtttctttaa gcacgataat ttaaaaggaa tacttgaaga
     6781 tttagtcaaa aaaggatatt taaaaaaaga gcatcctaaa cggcttgtaa aagaaactaa
     6841 tttgttgggc gaaataaaga caatacgcaa acaggaccgt agtttaccat taggttataa
     6901 tattgtatcg ggaaaactga gttttgaagt aaataaaatt ctatgtccca acgatatagc
     6961 acctacactt gtggcaatgg atatgaaaaa actttttgta gttgacaatg gaggtttgag
     7021 aacattgaca ttaaaagaag gcttgaaatt atttggattt cctgaaaact ataaattcaa
     7081 tataagtact gatgaaggat atgacttact tggtaataca gtaacagtac ctatagtaaa
     7141 agctgtttct gaaaggcttt taacaatttg gaaaaaacaa aaaacaagaa aacatgaaac
     7201 taacagcaca agaagttttc aacaagttaa tgcttgaaga taaaatttta gaagtaaaag
     7261 gtcaaataaa attctattta ggaaatgtca gcattgttgt aagacagcgt gatgttatag
     7321 gaaatattat gcaggagtgg cttgaaggct ggttgaatca aaatcaagtt ttgcacaaaa
     7381 taaacaccaa tacgcaaatg ccccctgact tttatcttaa tcctgatgac ttgacttctg
     7441 aattattgga agtaaaagca ttcaattatg atgcttctcc tggctttgat atcgcagatt
     7501 ttaatgctta tcaatctgaa atcattgagc aaccatttat gttacataca aaatatctga
     7561 tttttggtta tcaaatgcaa gatgatggtt atgttgtaat caagaaatta tggttaaaga
     7621 atgtgtggga aatttgccgt agaatggaaa aatggccatt aaatttacaa ataaaggaca
     7681 aagttgtaca taaaatcaga ccttgcactt ggtattcgca aggtcgaagt gttaaattta
     7741 aaccatttga atccttagaa gattttattt ctgcaatgga acaaacagta tatcaaaatc
     7801 ctaagacacg tgaaaaaagt ggagaatggt taaaagtttt tcttaagagt tatgaaaata
     7861 agtataatac caaattaaaa gtaccacgtt gggatgatat tgaagacaac tatataaata
     7921 aaaagtaaag ggcagttgcc tgacaacgtg tttgttttat ttgagctgaa tttggtgtgg
     7981 aaagttcttt gcttttaagt attttatatt gctggaatag agcattgttt caatgcccca
     8041 aacaaaacaa acacgtagct actactgcat catcaatctg tttccagctg cgcgccggat
     8101 catctcatcg tgcgtacgag gtcggattta tgataatgca cacttttgca gtttttcaat
     8161 gataagagac attcgctctt cccgcattaa tgggtgtaca tgaacactta ttatttcagg
     8221 aaaattttca ttgctcagaa ttttgacaaa ttcgtccttc agatattttt taacagcatc
     8281 atcagcatct gatacttctt tgacaatggt agtccggtta tcaatgatat atataatatc
     8341 ttcgaaatca tgactggtcc tgtaatctgt acctctgctg tgaaatgctt caagtttagt
     8401 agcgatgaaa taaggcgcag agagtacatg aatgatttgg ccgtggacct caattttttg
     8461 caacgattca aagcctggtt tataccagcg gttgctatcg cccaaggcgc ctgtctccga
     8521 aggcatgatg tcaatttcca tttggttata cagaaagctg cagagtgcat gacctgccgg
     8581 atttggagcg aatttcagtt ccagcaatct tttgttgagg tttacccaat cagtatacgt
     8641 tgccagtttg atggtcatgt caatatcgga tgtaggcctg atttcgtcgg cagccggatc
     8701 gtcggtatag acgctaataa cagcgcctcc tacaaaaacc attttttcgt ttagttcatg
     8761 caatgcgctg gcaacttcgg acaccattct cagattgata attttatttt ccatgtagaa
     8821 ttcttttttc aagctctttc aatgccagtt ctttctcgcg ggtacggcca actctgacgg
     8881 catcaaccaa cgccagtaat tcataaaggg cgctatcatt cagtgctgcg tccggcaccg
     8941 acgaataaag tggcgctatg ctatgtcctc ttacttttcc acttgcaaaa ggccacacat
     9001 attgttcatc gcttaatatt ttattactca gaggtggggc tgaatgagca gtaggtatac
     9061 cccttacaac gggcccgggc ttttgtggaa agacataact cagcccgtat ttaagaaaat
     9121 cgaaaaaagc gagtctcatt actttttttc ccaacagcag gccggcatat tgcattcgag
     9181 atgctgactg gcttatttcc gactggctca ttttcagtga atcagccaat ggcttttgct
     9241 gccaatggtc gctgccaatg gcgataatct ttagcagaat gactacatcc tgcggcttca
     9301 tttggttgtt tgatgacttc atgtgcgtaa tattgcatat tgcgatatgc aatattacaa
     9361 caaatatcat tgataaacaa atatattaca aaatatatcg catattgcga tatgcaatat
     9421 ttgagactta aaattgtcac ggattgagtg gtttgctcat ttcaactttt ttggtgcaat
     9481 aacatacaaa gcatcatccc gcttaccgca gtatcagatt attcgcggct gcgcgccgga
     9541 tcatctcatc gtgcgtagcg caaataatgg tgtggccttg tttgacaaga tgctcaaaca
     9601 aatgcagcag cctgagcgta tcggtcggat gcaggccgcc actgggctca tcaagcagga
     9661 aaagtgtttt tcctttggcg gaggcaatgc cctgaatcag cttcagccgc tgcaattcgc
     9721 cggtgctgag cgtgctcaga ttttgtccgg gcgaaatata gtcaaccccg cagcgggtgc
     9781 ataaatccgc aattgtcaga aacgatgcgg cttttgctgc tgttgtgttt ttttgtacaa
     9841 attcagctgc ttccgaaata ctcatattca atacatcgga aatggaaaat ccatccacca
     9901 caacattcag aatgtcgctc cggaagcggg ctccgttgca gctttcgcaa acggtttcgc
     9961 tgtcgctgcg aaaatccata ctggttttta tcactccggt tccgccacat tcggggcaac
    10021 gtccttcttt tgagaacacc gagaaatggg tcgctttcaa aggtaatttc aggcgtgcag
    10081 cttctgcggc aaaaatattt ttcagtgtat catacagtcc aagccatgtg gccggaatgc
    10141 tggccgcgct gcccgatgga acatcctgct gaatgaaaac aacggaatca aagttctcaa
    10201 agccgctgat gctgcggcat ttcacaggtc gtccggccat gtgcgaatca tacatcacat
    10261 attgcagcag actcgttttt ccgctgccac tgttgccggt aattacgttt aatgtcccgc
    10321 cggaaaaatc agcgtcgatg tttttcaggt tgttggcact ggcgcctgtc acctgtaaag
    10381 cctcgccgga tgtgctgtgc agtggtgtta cttttaaata ggtttgttca atttccgaaa
    10441 tatattccgt cgaagaaact tctttcacca gcctgccgcc ttgttttcct gctccgggtc
    10501 caagcacttt gatgcgatcg gccagttgaa tcatttcggg atggtgatcg gtcatgacca
    10561 gcgtattccc gcgctgcaca atgtttttca gcagttgcga aatacgcgaa caatcgcttt
    10621 tgcttaatcc gaacgaaggt tcatccatca caatcatgac gcccgaaagt tgtgattgta
    10681 ataatgccga catacgcagc cgctgaaact cacctcccga aagggtagcg gtcatgcggc
    10741 tcagcgcaat gtagccgatt ccgctttcaa tcagataatt caatcgttgt agaatttcgg
    10801 caacaatatt ttcgcttccc gttccgggtt gtatgtgctg aaaccacgat atggcctcgc
    10861 tggcgcacag gtcggttgtt tctccgatat gtctgttgtc aattctgtat tgcaaacgtt
    10921 cgtgatgaat ccggtagccg ccgcacgaag gacaagtcat gtcgctcatg accggaagaa
    10981 tctgctctgc gcgatgatcg ttgtgtacac gatgatactc ttcttccaca tgtccgcaga
    11041 agcccggcca tttcgatttc attttatgaa caccgctcac attgccgcgt ttgtaattcc
    11101 attccacatc gaatgattca tcgccgcaac catgcatcgc tatggaacgc gcatcagcac
    11161 tgagttcgga gtaaggaact gaaaaatcga tgccgtattt ttttccaaca gcgagtaaag
    11221 cggccacata ttgtccgtcg ggttctccat aaaactttcc gggcttgctg ccgtcaatcg
    11281 cgccttgtat cagcggtttc gaagggtcgc tgactaattt ttccgcgtcg cattgggtga
    11341 taaatccaag tccgtgacaa tcgggacagg cgcctgcttc gttgttgaat gaaaaagcat
    11401 tggaataatg cggacaagtt gtttcaccgt ttttcgaaaa gcggctgtag agcaggcgat
    11461 acagatcgta caatccggtg taggtgccaa ctgtggagcg tggattgccc gatgcacttt
    11521 ttttctccag cgcgatggtc ggcatcagac ctgtgtattc atcgagtgcg gcattgcctg
    11581 cttttttcag ctgcgagcgg atgcggaacg aaagtccgtc catcagcgca ttctgcgaag
    11641 cagcatataa cgtatcgaaa accagtgatg actttccact gccacttggc ccgcagacag
    11701 cgtttacaac gttttcgtga aattgtattt ctgcaatgtc gaggttgttt gtgtgaacct
    11761 gtttcagtgt aatgtcactg aaagctgttt tctgattttg caatgaaacc tctgaagccg
    11821 gctttggttc ggcaaaatca gaaagtgctt gtgcagtttt tgaattctta tttgaaatta
    11881 attcggcggg aacgcccgaa aacactacgt ttccgccatg tgtgccgctg cctggtccga
    11941 gttcgcaaat gtgatcggcc tgtgcaatca tgcgcagatc gtgttcggca gcaatcaccg
    12001 tatgaccttt ttgagtgagt tcgcgcaggg ccgaaatcag attgttcaca tcgtgcggat
    12061 gcaggccggt agcgggttca tcgagcagaa acaagaccga tccgcttgtg ccgcggacca
    12121 gttccgaagc cagtttcacg cgttgtgcct caccacccga aagcgtggtg gacgactgtc
    12181 cgagcttgat ataacccagt cctaaatcga gcattggcct ggtatatttt aaaacttttg
    12241 gttcggctga aaagaattca tgtgcttcgg ccacggacaa ttcaagcaca tcgtgcatgt
    12301 ttttttcacg aatctgaatg tgcagcacag tctccgaaaa gcgcatgcca ccacaggttt
    12361 cgcagggtat ttccacgttg cccagaaaat gcatgccgat ttgtttcagc cctgcaccca
    12421 tgcaatcctc gcaccggccg ccgggtacag ccagcgagaa atgtgttttg gtgagtttgt
    12481 tttctttggc agaagcagtc gaagcataca aatcgcgcaa tatatctgac aatccggtgt
    12541 aggtggccgg attgctgcgc ggactgcgtc cgaacggatc ggcatcgatg tgaatgattt
    12601 tgctgaatcc ggcttgtgtt gcctgatcgt gcactacgct cagcaaactt gttttaccgc
    12661 tccccgacac gccgcaaagc acattgaatg cattgcgcac aaaggaaacg gaaatatttt
    12721 tcaaattgtt tttatttgct tgatgtatct caaacgcagg attggagagc ttttctgatt
    12781 tcagctccgg aatattgtgc agccatttct gtgtgggact gtcggaaata ttctgagtaa
    12841 agtattgcga agcgttaccc gaaaacaaca cttcaccacc gtcatttccc gcaccgcgac
    12901 ctatttctat gatgtagtcg gctgcagcaa tatttttttc atgatgatcg accacgatga
    12961 cggaattgcc atgatcgacc agacgacgca acactttcat catgctttgc tgttcgctgt
    13021 cgtgcagtcc ggctccgggt tcgtcgagga tatataaaat gttgcggata cctgaagtgg
    13081 tgtaggccgc cagccgcaga cgattgagct caccggtgga cagggtggag gattcgcggt
    13141 taagttgcag atagccggcg ccgagctcaa gcagcgtggt tgtatttttt agtatctgct
    13201 ccgaaatctg catcaatcct tcgttttcag aagtcagttc tgtaaaggtc cgatgaattt
    13261 cttcgatgct catgcggtga aaatcatcga tgtttttgtt gttccattca aacgacatgg
    13321 cttcgttgcg caggcgagtg ccactgcacg actggcaggt gaaggagcgt gcgaagcgca
    13381 ggatgttcgg attgcggtcg cgcttcagaa tttcttccat cacgggaaca atacctttgt
    13441 aaaatccctc ctcgcgcggt tttgcggtga ttccagacca cttcaggcgc gactcgaggg
    13501 tgtgcttgcc aaaaaggatt ttaatcttct cagagccgtt taaaacgata tttttctgag
    13561 cgtcggtcag gtccttccac ggaatatcga ctgaaaagcc ttcggcgaca caaaccttgt
    13621 tcagctcttc catggtgacc tgcgaataaa tgatgtagcc attcggtgtg gtcagcaccg
    13681 atgcgccttc gcgcagggtc ttggtgtcat cgccgatgag aagttgcgga tcaatatggt
    13741 cttcgacgcc caggcctttg cattgcgggc attgtcctgc tgggctgttg aatgaaaaca
    13801 gactgcggtt ggattctgta acgctgtcgc tgtgagcata tcgggcaaac agtaaacgca
    13861 gcagatccca cagccctgtg atggtgccga ctgtcgagcg cggactggag gcgttgcctt
    13921 gctgattgag catcagcacg gcgggcagac cttcgatctg atcggcttcg ggccggccca
    13981 gtttgatgtt ctgatgatag ctgcctggca ggaaatgttc gtaaaacagc cgctggcctt
    14041 cttttccaat aacatcgtat atcagcgacg acttgccgct gccgctcaca ccacacgcca
    14101 caaccagctt tccgtgcggg atttcgaggc tgatgttttt tagattattc gccctcgcgt
    14161 tgcggatgat gatggtgttg ttcatgatgc aaaaataaga aaatgtgtta tgaaacgccg
    14221 aaggcatata aaaagctgcg acttgtcgcg tttcacgcga agctccacca gatactcctc
    14281 cgcatcctgc ggagtaaact ccggagtaaa ataataatag cccgaagaat tcacgtttta
    14341 cgtaatgccc tgaatgctga gcttgcgaag ggcagggctg cgtggacgta gaagacgcga
    14401 agcacctttg agatgtatcg tcacgcggct tcagacgcac gcacattttt gggatagtat
    14461 ttcggaaatg aaacaactgg agctttcatg ttcagccgcg ccaccagctc cttcatctcc
    14521 gcttcattct tcccgatttc ggccaccagc ctgaattgtg cgcgggggcg ctgcagcatc
    14581 taacagcctg tccgcccttt ggtggaattc cgtgttcgct ctgcttcgca actttatgtc
    14641 tggtcccgct tcaagcggga taaattccac accaaaatgt tgggtgcatt acagacattt
    14701 tagcgtcaga attttttttt atatccttgt gacagcattt gttcttcaga tcaaatcgtg
    14761 atagcgacgc gacatcaaaa atctgatccg cagctatgcc gcggcgtagg acgtaaatag
    14821 ggcctgctga aaaacactta acactcacag aattagaaac atattcatca aattgggcat
    14881 tgcaaatgca agtgtattta tcgggttaga aaaataacca tgcacttgag aaaccgttgc
    14941 tgcaattttt gaagtcaaac aaacattatt tctttttttt
//
LOCUS       DAOPKE010000065        14962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4191_length_14962_cov_13.090763, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000065 DAOPKE010000000
VERSION     DAOPKE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_14962_cov_13.090763"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..>1454
                     /locus_tag="PKY63_08900"
     CDS             235..>1454
                     /locus_tag="PKY63_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF00770.1"
                     /translation="MKNRQIANFAKKISKSGLGRILVFTGARQTGKTTLVKKLFPEYS
                     YLSIEDPLTRADFTGLTAARWHALYPKAILDEIQKEPVLIESIKSVYDQFSEPRYVLL
                     GSSQLLLMEKVRESLAGRCVIIEMFPLTLPEIKADEPEKTQLSLFQKMLTTPSQKMEF
                     FPSFNLDPEMAKKQTAYSHYCTFGGYPALTDAQLSDEQKYIWLGQYVRTYLERDIRDL
                     ASFRELEPFVILQRALAVQTAQTINASTLATQVGLSSKTVQRYIQYLNISYQTISLQA
                     WSRNQTKKLTRAPKIHYLDHGVLSGILQKKGGMTGAEYESLIVAEIYKQIKTLNIQPM
                     LYHLRIQGGREIDLLIELPEGYIAFEIKMSQRVSASDASHFSGLDKILDKPLLHSFVI
                     SNDVKTQQFPNGSTA"
     assembly_gap    1455..1554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1555..2478
                     /locus_tag="PKY63_08905"
     CDS             <1555..2478
                     /locus_tag="PKY63_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPF00771.1"
                     /translation="KKVFYNRNIHIEPTNICVYNCRFCSYRANSEADSYIFPGEKIIE
                     KIATVAGDITEVHITGGVHPDWGIYFFADLLKKIKTNFPHIHIKAFTAVEVAYMCNID
                     KLEIADGLRVLKDAGLSSLPGGGAEIFDESVRAIISPEKPSGELWLNVHRAAHLNGLP
                     SNATMLFGHIESRTQRITHMAAIRTLQDETHGFNCFIPLKFRRANNRMSDVPETPVLE
                     DLKTYAIARIFLDNIPHLKAYWPAVGRDFAQISLHFGVDDLDGTINDSTTIYTRAGSS
                     ENPDMTTPELQRLITEAGFRPAERDSVYNEL"
     gene            3011..3670
                     /locus_tag="PKY63_08910"
     CDS             3011..3670
                     /locus_tag="PKY63_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00772.1"
                     /translation="MPKKQILITAATAMELDFLLARSAEKEGLPVTENRTTQFTLLVS
                     GIGSALTAFSLSKILQHKKFDAAYQLGIAGSFKNDLLVGSIVKVSSDIFADLCLSDNG
                     KPIHEATFSDFRTYPFTEGKLIPDLSAFKQIDLPGASAITVNSVTATSDKREFWLQRY
                     NPGIETMEGAAFYYACMKENIPCVQIRAISNMVSSVSGKWKTRESIENLASFFLSLYK
                     M"
     gene            3675..3914
                     /locus_tag="PKY63_08915"
     CDS             3675..3914
                     /locus_tag="PKY63_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00773.1"
                     /translation="MQTIRLKVNDKVYDKLMWLLGKFSKDEVEIISEESLFNETKTFL
                     RAELDEIDKGKAEFTGVEEAEIYLEKVIRKHENRH"
     gene            3898..4197
                     /locus_tag="PKY63_08920"
     CDS             3898..4197
                     /locus_tag="PKY63_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF00774.1"
                     /translation="MKIVIKKSFLQRLGRQVDFIANDSVKRARKFSKEILEQINKIPD
                     NPYINRKSIYFNEESIRDLIYKGYTVVYRICDDEIQVFGFVKYQELPIDGVNNTE"
     gene            4252..5088
                     /locus_tag="PKY63_08925"
     CDS             4252..5088
                     /locus_tag="PKY63_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPF00775.1"
                     /translation="MPALSLAISPCPNDTLMFDALVNSKIASGEISFNTEYHDIEQLN
                     NGLMSGKWEVAKMSAAAFPLVMKDYVMLQTGMAMGFGNGPLIVAKNAEIDLNSFKDTM
                     LIPGIHTTANLLLTKFFSNITNKNEVLFSDILPLLEAGKAAAGLIIHESRFTYPQYGL
                     HKIADMGELWFHQTKIPLPLGVIVARRSLGFELIRKLEMLLQESVIYGLQHRDDAMSY
                     IRSHAQETDDDVINHHIDLYVNQFSSEPGQDGLQAVLTLLQSLPENHSLSLHDIFSPW
                     VN"
     gene            5076..6050
                     /gene="rfaD"
                     /locus_tag="PKY63_08930"
     CDS             5076..6050
                     /gene="rfaD"
                     /locus_tag="PKY63_08930"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPF00776.1"
                     /translation="MGKLNILTGAAGFIGSVLGRQLMEYGMENIIAVDDFSKTAKANN
                     FEGRKYKDFIHRDKLGEFLEQHKTDIDAVFHIGARTDTTEFDVEIFNKLNLNYTKDLW
                     KFCAERNVPFIYASSAATYGDGTFGYKDDHNIVDKLKPLNPYGESKNDFDKWALQQTQ
                     APPFWYGLKFFNVYGPDEYHKGRMASVIFHAFNQIGETGRMKLFRSHKPEFADGMQMR
                     DFIYVKDIASIISFLFEHRPESGLYNAGTGKARAFLHLVQAVFAALELKPEIDFIDTP
                     EDIRDKYQYFTEADMSKLISKGYSKPFVSLEDGVKEYVCGYLKDHKYF"
     gene            6054..6815
                     /locus_tag="PKY63_08935"
     CDS             6054..6815
                     /locus_tag="PKY63_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00777.1"
                     /translation="MKKISLLIIALTLAFANNLTQAQDTTKKADRITNVHIQYGFHLP
                     GGDMNERYGMNQVAGISTSFKTSTNWMFDADFSYIFGGNIKIHDSLFANIANDDGNII
                     DGNGQFAELYTYERGYYITLGVSKIFPWLAANKNSGVTIGAGAGFMQHKIRVYNPDNI
                     APQVCGEYAKGYDYLTNGTALKQFVGYTFFAPKKVYSFKAGFEFVEAFTQGRRDYLFP
                     IGGPDTEKRIEFLYGFKLMWTIPFKKSTGETYYYF"
     gene            6859..8049
                     /locus_tag="PKY63_08940"
     CDS             6859..8049
                     /locus_tag="PKY63_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPF00778.1"
                     /translation="MIRLAALSGNDKAKHWLNGRKSQKHLFSENHNNNQWLWFHVSSL
                     GEFEQGRPLIEHYRSHNPEYKILLTFFSPSGFEVRKNFKEADLVLYLPSDTKCHARRL
                     LKNFNIKTAFFVKYDFWFNYLHELKKSDIPLYLVSGIFRKNQHFFKWYGRWQRRQLAA
                     FTHFFIQNDTSAELLRQYGFSNYTITGDTRFDRVVKVAKDAKSFPEIEQFVQQQPVYM
                     AGSSWEPDEKFIIQLIQKKTNLKFIIVPHEINEERIRNLLQSLPCKASLFTEKNLPDF
                     NNNRILIINTIGMLSSLYRYATIAHIGNGFGSGIHNTLEAAVYGVPVIFGPNYHKFQE
                     AKDLIECGGGFSFATDAEYHTLCDSLLNDYAACKTASEAAQQYVAAHAGATSVIVDTI
                     KLQS"
     gene            complement(8050..8541)
                     /gene="fabZ"
                     /locus_tag="PKY63_08945"
     CDS             complement(8050..8541)
                     /gene="fabZ"
                     /locus_tag="PKY63_08945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPF00779.1"
                     /translation="MELKLPEFDLNAKPLFDVVEVQRFLPHRPPFLFVDKIMLMDDKS
                     IVGVKNVTMNEPFFVGHFPGAPVMPGVIQVEAMAQVGGVLMLNTVPDPENYLTFFLTI
                     DGIKFRDKVVPGDTLVFYLELLSPIRRGICEMAGNGYVGGKLVLEATMKALIRKMTSE
                     NAK"
     gene            complement(8522..9454)
                     /locus_tag="PKY63_08950"
     CDS             complement(8522..9454)
                     /locus_tag="PKY63_08950"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPF00780.1"
                     /translation="MSRQKTIREPIEIQGKGIHTGCMTTMRFLPAAPNHGYSFCRVDL
                     PGKPIVRALAQNVFDTSRGTSIREGDAIVRTVEHVLAAVLGCDLDNIMIEINSEETPI
                     LDGSSAPFTNILQKAGIVEQDAIRIVYNVQKKVYLKDTDKDVEMWLVPDDGYRISTMV
                     DYNSKVLSSQNALLKSVSDFNAEFSKARTFVFLSEVEMLLQRKLIQGGDIKNAVVFVD
                     RMLSQEELDHLAKVFNQPKVNVLKEGVLNNIELNYPNEPARHKTLDVFGDLCLVGCRF
                     TGHVIANKPGHKTNVEFAKMIQNQIQKTYGIEIA"
     gene            complement(9483..10496)
                     /gene="lpxD"
                     /locus_tag="PKY63_08955"
     CDS             complement(9483..10496)
                     /gene="lpxD"
                     /locus_tag="PKY63_08955"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPF00781.1"
                     /translation="MEFTAAQVASYIGGKVDGDAAAKLSNFAKIEQGKAGDLCFLANM
                     AYEQYIYSTEASAVIVAEDFVPSKKVNTALIRVSNPYEAFAKLMALAESQKPRNKGIS
                     ERAFVAPGVLIPDTCLVAEFAVIQSGAVIGENVQIHPQVFVGENVKIGNDVILYPGVK
                     IMHDCEIGDRCILNPGAVIGTDGFGFAPDAQGHFSKIPQLGNVIIEEDVEVGANTTID
                     RATMGSTRVGKGTKLDNLIMVAHNCEIGQHNVFAAQAGVAGSTKIGDHNQVGGQVGFA
                     GHLKIGSYCRFAAQSGIMDNLSDHSAVMGAPAVSATRFFRIYAVFKKLDELAFRVNAL
                     EKK"
     gene            10768..14901
                     /gene="cas9"
                     /locus_tag="PKY63_08960"
     CDS             10768..14901
                     /gene="cas9"
                     /locus_tag="PKY63_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HPF00782.1"
                     /translation="MKKILGLDLGTNSIGWALVNEAETADEKSSIIKLGVRVNPLTVD
                     EQQNFEKGKSITTNADRTLKRSMRRNLQRYKLRRENLISILTEHNLISESTILSENGN
                     STTFETYRLRAKAATEEISLEQFARVLITINKKRGYKSSRKAKGTDEGELIDGMEVAK
                     KMYEENLTPGQLCLKILETGKRNLPVFYRSDLQSEFDLIWNKQKEFYTDILTNELKES
                     LKGKNEKQTWAICQNPFKIVGIKRATKGEELKKENFSWRVKAIKEKIDLEELAVVLQK
                     INGQIKSSSGYLGEISDRSKELYFTKQTVGQYQMAILAKNPNASLRNMVFYRQDYLDE
                     FEKIWETQSQYHKELTTELKKEIRDVVIFFQRRLKSQKGLISFCELENQQKSVIVDGK
                     HKTITIGRKVCPKSSPLFQEFKIWQILNNLEITNKISGEKILLEKEEKDILFLELSIK
                     EKLSKDEVLKLLFNNYKELDLNYKSVEGNKTQAALFTAYQQIIEASGHGENDFSKMKY
                     ADVIEIVEGVFTSLGFNTEILKFNSDLAVNELEKQPHFQLWHLLYSYEGDNSNTGTQK
                     LIEKISAKFGFDKEYASIISKISFQDDYGSLSTKAISKILPYLKDGDEYSKACFHAGY
                     NHSKSSLTREEIENKILKDKLDILKKNSLRNPVVEKILNQMINVVNAVVETFGKPDEI
                     RIELARELKKSAKEREDMTKAINESSAAHEKIKLLLQKEFGLSHVSKTDIIRYKLYSE
                     LEPRGFKTLYSNTYISPEKLFSKEFDIEHIIPQSRLFDDSFSNKTIECRSANIEKGNE
                     TAFDYIAAKYDDNYLQDYLSNIEVLFKNGNISKAKYNKLKMRGKDIPTDFINRDLRDT
                     QYIARKAKSILEDLVRNVISTSGSITDRLREDWQLIDVMQELNWDKYDKLCLTEIIEG
                     RDGQRIRRIKEWTKRNDHRHHAMDALTIAFTKPSYIQYLNNLNARSDKSSSIYGIEQK
                     ELFRDKLGKLRFKPPMEIDLFRTESKKHLQNTLISIKAKNKVVTSNINITKKPGPDNK
                     RKQLTPRGQLHNETVYGQIHQYVTKEEKIGAQFSVEYVQHVAKKTYRDALLMRLSENN
                     NDPKSAFTGKNSMSKNPIFIDEAHSMTVPEKVKLVFKEPVYTIRKEISADLKIEKVID
                     VKIRNILEARLKEFNNDAKKAFSNLDENPIWLNKEKGISIKRVTITGVSNAIPLHDKK
                     DHHGNIITDAQGRKQAVDFVSTSNNHHVALYKDQNGNLQEKVVSFFEATTRANQGLPI
                     IDKEYNNTEGWKFLFSMKQNEYFVFPNESSGFVPSEIDLLNPENYFLISPNLYRVQKF
                     GSLLSGFWFRHHLETNVDAMKELKGITYKVIQSANNLNNLIKVRLNHIGQIVSVGEY"
BASE COUNT         4948 a         3057 c         2847 g         4010 t
ORIGIN      
        1 tttaaagctc atccaaactc aaaataaagc tcaaaaaagg cgaagtttaa gctttattca
       61 gaaaaaaatg agcataaaac tatgaatgaa gaatattcag atcgattttc gccgtaatat
      121 ttggagttta attccaattt tcgccgtgat ttttggaatt caattccaat tttcgccgtg
      181 atttttggaa tttcgagaaa aagattgtat atttgctgtt ggaaatcttc atatatgaaa
      241 aacagacaaa ttgccaattt tgcaaagaaa atcagtaaat ccgggctcgg tagaattctt
      301 gtttttacag gagcccgaca gactggcaaa acaacgcttg tcaaaaagct gtttcctgaa
      361 tattcatatt tgtcaatcga agatccgctt accagagctg atttcacagg tcttacggca
      421 gccagatggc atgcgctcta cccgaaagcc atacttgatg agattcagaa agaaccagtt
      481 ttgattgaaa gtatcaaatc cgtctatgac cagttttccg aaccacgata tgttttactt
      541 ggttccagtc agcttctgct tatggaaaaa gtacgcgaaa gtcttgccgg acgatgtgtc
      601 attattgaaa tgttcccatt gacactgccc gaaataaaag cagatgagcc agaaaaaacg
      661 cagttgtcac tatttcagaa aatgctcacc acgcccagtc agaagatgga attttttcca
      721 tcctttaatc tggatccgga aatggcgaaa aaacaaacag cctactcaca ttattgcact
      781 ttcggtggtt atccggccct cacagacgca caattatcag atgaacaaaa atacatctgg
      841 ctcggtcaat atgtgagaac gtatcttgaa cgggatatcc gcgacctggc atctttccgc
      901 gaacttgaac cgttcgtaat tctgcagagg gcacttgctg tgcagactgc acaaacaatc
      961 aacgcttcaa cactagcaac acaagttggc ctcagctcaa aaacagttca acggtatatt
     1021 caatacctga atataagtta ccagactatt tcgctgcagg catggagccg aaatcaaaca
     1081 aaaaaactga caagagcgcc aaaaatccat tatctcgacc atggcgttct atcaggaatt
     1141 ttacaaaaga aaggcggtat gaccggtgca gaatatgaga gcctgattgt tgctgagatt
     1201 tacaaacaga ttaaaacgtt gaatattcaa ccaatgcttt atcacttgcg aattcaggga
     1261 ggaagggaaa ttgatttact gattgaactt ccagaaggtt acatcgcatt tgaaattaaa
     1321 atgtctcaaa gggtttctgc atcagatgca agccatttct caggacttga taaaattctt
     1381 gataaacctt tactacattc tttcgtaatt tcgaacgatg taaaaacaca gcaattcccc
     1441 aatgggtcta cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaag
     1561 gttttttaca accggaatat tcacatcgag ccgacaaaca tctgtgtgta taattgccgt
     1621 ttctgcagct acagagccaa ctccgaagca gacagttata ttttccctgg agaaaaaatc
     1681 attgaaaaaa tcgctacggt tgctggcgac attaccgaag tgcacatcac aggcggtgtt
     1741 caccctgact gggggattta tttctttgct gatttactga aaaaaataaa aacaaatttt
     1801 ccgcacatcc acatcaaagc gtttactgca gtcgaagttg cgtacatgtg caatattgat
     1861 aaacttgaaa ttgctgatgg cttaagagtt ttaaaagacg cgggattatc atctctcccg
     1921 ggtggcggcg cagaaatatt tgatgaatcg gtacgtgcaa tcataagtcc tgaaaagcca
     1981 tcaggggaat tatggctgaa cgtgcaccgc gccgcgcact taaacggact tccaagcaac
     2041 gccaccatgt tgttcggtca cattgaaagc cgcacacagc gcattacaca catggctgca
     2101 attcgcacat tgcaagacga aacacatgga ttcaattgtt ttattccgct gaaattccgc
     2161 cgtgccaaca atcgcatgtc cgatgtgccg gaaacacccg ttctagaaga cctgaaaaca
     2221 tacgccattg cacggatttt tctcgacaac attccgcatc tgaaagcata ctggccggca
     2281 gtggggcgcg attttgcaca gatttcgctg catttcggag tcgatgatct cgacggcacc
     2341 atcaatgatt cgaccaccat ttacacccgt gccggcagca gtgaaaatcc ggacatgacg
     2401 acgcccgaat tgcagcgcct gattaccgaa gccggtttcc ggcccgcaga gcgcgattcc
     2461 gtgtacaacg aactttgatt ttcacaatga cattccatag tccgcaacgc agtgaaggac
     2521 atggaatagt ccgcagtgaa tcggataaat ggtttcatgc ccattcgctc ttataccatt
     2581 aagaaattta gtagtccatt attttgcgta ctccggctca aaaagcatta ttgatgtttg
     2641 taatatttgt cgtggaaact atatttcaga caactctgac ttccggagga agtcgaataa
     2701 taatagcgtc cggcgctagc cggatgtaaa agaatatcca atatacggcc ctgcaggggc
     2761 cgaatacaac gagatgcgtt tttgtttttc gaaattaatc cgttggaaat ttcgttaaat
     2821 tcgacctcgc tgaggtcagc ccggttgtct tctcttaccc ggacttcgtc cgggctatta
     2881 ttgtttgacc tgcgctacag gtcacggctt ttacaagaat aaatgaaccc tttattttta
     2941 tcattagaat cccacaacag tccgaaacat tatcgcagca tttgtgtatc tttggcttga
     3001 atttttttag atgccaaaaa aacaaatcct tatcactgcg gccacagcca tggagctgga
     3061 ttttcttctc gcacgaagtg cggaaaaaga aggattgccg gttactgaaa acagaaccac
     3121 acaattcaca cttcttgtaa gcggcatcgg gtcagcgctg acagcttttt cactaagcaa
     3181 aattctgcaa cataaaaaat tcgatgccgc atatcaactg ggcattgccg gcagcttcaa
     3241 aaacgattta ctggttggct ccattgtaaa agtcagcagt gacatttttg cagacctttg
     3301 tctgagcgac aacgggaaac ccattcacga agccacattc agcgatttca gaacatatcc
     3361 gtttactgaa gggaaactta ttccggattt aagtgcattc aaacagattg atttgccggg
     3421 agccagcgcc ataacggtca attcggtgac agcaacatcc gataagcgcg aattttggct
     3481 acaacgctac aatccaggca ttgaaaccat ggaaggcgct gccttttatt atgcttgcat
     3541 gaaagaaaac attccctgtg tgcagatcag ggccatttca aatatggtaa gcagcgttag
     3601 tggcaaatgg aaaacacgtg aatcaattga aaatcttgca tcttttttcc tatctttgta
     3661 taaaatgtaa attcatgcaa acaatacgcc tcaaagtaaa cgacaaagtt tacgataagc
     3721 tgatgtggct gcttgggaag ttcagcaagg atgaagtcga aatcatttca gaagaatctc
     3781 tttttaatga aacaaaaacg tttctgcggg cagagctgga tgaaatagac aaaggcaaag
     3841 ctgaatttac aggcgtagaa gaagcagaaa tatatcttga aaaagttatc agaaagcatg
     3901 aaaatcgtca ttaagaagtc ttttcttcaa cgactgggaa ggcaagtcga ttttatcgca
     3961 aatgacagtg tcaaaagagc acgtaaattt agtaaagaaa ttcttgagca aattaataag
     4021 attcctgata atccatatat caacaggaaa tcaatatatt ttaatgaaga atcaatccgg
     4081 gatctgattt ataaaggata cacggtcgtt tatcgaattt gtgatgatga aattcaggtt
     4141 tttggatttg taaaatacca ggaactaccg attgatggag taaataatac agaataaaac
     4201 attgaaataa taaatacttg aaaaactcgt tacttaaaat ctaattatat tgtgcccgca
     4261 ctctctctcg ccatatcgcc ctgtccgaat gacactctga tgtttgacgc attggtgaat
     4321 tctaaaattg catcaggcga aatttcattc aacacggaat atcacgacat cgaacaactc
     4381 aacaatggct tgatgagtgg caaatgggaa gtggctaaaa tgagcgccgc tgcatttccg
     4441 ctggtcatga aggattatgt gatgctacag accggcatgg ccatgggttt cggcaacgga
     4501 ccgctgattg ttgccaaaaa cgctgaaatt gatttgaatt cattcaaaga caccatgctg
     4561 attcccggca ttcacaccac agcaaatctg ttgctcacga aatttttttc aaatatcacc
     4621 aacaaaaatg aagttctttt cagtgatatc ctgcccttgc tcgaagctgg aaaagccgca
     4681 gcaggactca ttattcacga aagcagattt acgtatccgc aatatggttt gcataaaatt
     4741 gccgatatgg gcgaactgtg gttccatcag acaaaaatac cattgccgct tggagttatc
     4801 gttgcaaggc gcagtctggg atttgagctg atacgaaaac tggaaatgct gctgcaggag
     4861 tctgttattt atggactgca gcaccgcgat gacgctatgt cttatatccg cagccacgca
     4921 caggaaacgg acgatgatgt catcaaccat cacatcgatc tgtatgtaaa ccagttctcc
     4981 tcagaaccag gccaggacgg gcttcaggca gtattgaccc tgttgcagtc attgcctgaa
     5041 aatcattcat tatctttgca tgatattttt tcgccatggg taaactaaac attcttaccg
     5101 gtgctgccgg tttcatcgga tctgtactcg gtcgccagtt gatggagtat ggtatggaaa
     5161 atataattgc cgttgatgac ttctcaaaaa cagccaaggc aaacaatttt gaaggcagga
     5221 aatacaaaga cttcattcat cgcgataagc tgggtgaatt tcttgagcag cataaaacag
     5281 atatcgatgc ggtttttcat atcggagccc gcacagatac tacagaattt gatgttgaga
     5341 tatttaataa actcaatctc aattacacca aggacctctg gaaattctgc gccgaaagaa
     5401 atgttccgtt catttacgcc tcatctgcag ccacatacgg agatggcacc ttcggataca
     5461 aagacgacca taacatagtt gacaagctga aaccattgaa tccatatggc gaaagcaaaa
     5521 acgattttga caagtgggca ttgcagcaaa cacaggcacc tcctttctgg tacggattga
     5581 aattcttcaa cgtgtatggt ccggacgaat atcacaaagg ccgcatggcg tcagtgattt
     5641 ttcatgcatt caatcagata ggagaaaccg gacgcatgaa acttttccgc tcgcataagc
     5701 ccgaattcgc cgatggaatg cagatgcgcg acttcattta cgtgaaagac attgcttcca
     5761 tcatttcgtt tcttttcgaa caccgccctg aaagcggctt atacaatgcc ggcactggaa
     5821 aagcacgagc gtttttacat cttgttcaag ctgtttttgc agcactggaa cttaaacccg
     5881 aaattgattt tattgacacc cccgaagaca tccgcgataa ataccagtat tttaccgaag
     5941 ccgacatgtc taagctgatc tcgaaaggat acagcaaacc ttttgtcagt cttgaagatg
     6001 gcgtaaaaga atatgtatgc ggctatctga aagaccataa atatttttga aaaatgaaaa
     6061 aaatttcatt actaattatc gcactgacac ttgctttcgc aaataatctc acccaggcac
     6121 aggatacaac aaaaaaagct gacagaatca caaacgttca tattcagtat ggatttcatt
     6181 tacctggcgg cgatatgaat gaacgatatg gcatgaatca ggttgcagga atttcaacat
     6241 cattcaaaac cagcaccaat tggatgtttg atgcagactt cagttatatt ttcggaggca
     6301 acatcaaaat tcacgacagt ctttttgcca atatagccaa cgacgatggc aatatcattg
     6361 atggcaacgg gcagtttgca gaattatata cttacgaacg tggctattat attacactgg
     6421 gcgtttcaaa aatatttccg tggcttgcag caaacaaaaa cagtggcgta accatcggtg
     6481 caggtgcagg tttcatgcag cataaaatca gggtttacaa tcccgacaac attgcaccac
     6541 aagtctgtgg agaatacgcc aagggatatg actacctgac caacggtact gcgctgaaac
     6601 aatttgttgg ctatacgttt tttgccccca aaaaagtgta cagcttcaaa gccggttttg
     6661 aatttgttga agcatttaca cagggacgcc gcgactattt attcccaatc ggtggccctg
     6721 acacagaaaa gcgtatagaa tttttatacg gattcaaatt aatgtggacc attcctttca
     6781 aaaaatcgac cggcgaaact tattattatt tctgaaatgc gttgtattta ttcaatctcc
     6841 gtccacattt atacctttgt gatccggctt gcagcactaa gcggaaacga caaggccaaa
     6901 cactggctta acgggcgaaa atcgcagaaa catctttttt ctgaaaatca caataataat
     6961 caatggcttt ggtttcatgt gtcatcactc ggcgaatttg aacaaggacg cccgctgata
     7021 gaacactacc ggagccataa tccggaatat aaaattttac tcactttttt cagcccgtct
     7081 ggatttgaag taagaaaaaa tttcaaagaa gccgatctcg tcttgtatct gccttcagat
     7141 accaaatgtc atgcacgcag actgctgaaa aatttcaata tcaaaactgc attttttgtc
     7201 aagtatgatt tctggttcaa ctatctgcat gagctgaaaa aatcagatat tcccctctat
     7261 ctcgtttccg gaatattcag aaaaaaccaa catttcttta aatggtatgg gcgctggcaa
     7321 cgcagacagc ttgcggcttt cacgcatttt tttattcaaa atgacacttc ggctgaatta
     7381 ctcaggcaat acggtttttc aaactatacc atcaccggcg acacacgttt cgaccgcgtt
     7441 gtaaaagtag caaaagatgc aaaatcattt cctgaaatag aacagtttgt tcagcaacag
     7501 ccagtatata tggcaggttc atcatgggaa cccgatgaaa aattcatcat tcagttgatt
     7561 cagaaaaaaa caaatttaaa attcattatt gttccccacg aaataaatga agaaagaatc
     7621 cgaaatctcc tgcaatcgct cccatgcaag gcatcgctgt tcaccgaaaa aaaccttccg
     7681 gatttcaaca acaatagaat tcttatcatc aacaccatcg gaatgctgag cagtctatac
     7741 cgttatgcca ccattgctca tatcggaaat ggcttcggga gcggaattca caatactctt
     7801 gaagcagcag tatatggcgt tccggttatt tttgggccca actatcataa atttcaggaa
     7861 gccaaagatc tgattgaatg cggcggcggc ttttcatttg ccaccgatgc ggaatatcat
     7921 actttgtgtg actccttact caatgattat gcggcctgta aaaccgcatc ggaagcagca
     7981 caacaatatg ttgcagcaca cgcaggagct acatcggtaa ttgttgatac aataaaacta
     8041 caaagttgat tatttcgcgt tttcggatgt cattttcctg ataagcgctt tcatcgtggc
     8101 ttccaaaaca agtttgccgc caacatatcc gttgccggcc atttcacaga ttccacgacg
     8161 tatcgggctt agaagctcaa gataaaaaac cagtgtgtca cccggaacta ctttgtcgcg
     8221 gaattttata ccatcgatgg tcagaaaaaa agtaagataa ttttccggat ctggcaccgt
     8281 attcaacatc agaacgccac ctacctgagc catggcctca acctgaataa caccaggcat
     8341 taccggtgca ccggggaaat ggcccacgaa aaagggctcg ttcatagtca catttttcac
     8401 accaacgatg ctcttatcat ccatcagcat gattttatca acaaataaaa acgggggacg
     8461 gtgaggcaaa aatctttgaa cttccactac gtcaaacaaa ggtttggcat tcaagtcaaa
     8521 ttcaggcaat ttcaattcca taggtcttct gtatttgatt ctgaatcatt ttcgcaaact
     8581 caacattggt tttatgtccc ggcttattgg caataacatg gcctgtaaaa cggcatccaa
     8641 ccaaacataa atcaccaaaa acatccaatg ttttatgacg cgccggttca ttcggataat
     8701 taagttcaat attattcaaa acaccttcct tcaacacatt cactttgggc tgattgaaaa
     8761 ccttagccaa atgatccagt tcttcctgcg acaacatgcg atcgacaaaa acaacggcat
     8821 tttttatatc tcctccctgt atgagtttgc gttgcaacaa catttcaact tcggaaagaa
     8881 aaacaaatgt tcttgctttt gaaaactctg cattaaaatc tgaaacgctc ttcagaagcg
     8941 cattttgtga tgaaagcact ttgctgttgt aatcaaccat cgttgaaata cgatagccat
     9001 catcgggcac cagccacatt tccacatcct tatctgtatc tttcagatac acttttttct
     9061 gaacattgta cacgatgcgg atagcgtcct gttcaacaat accggctttc tgcagtatat
     9121 tggtaaaggg ggctgaactg ccatcaagta tcggggtttc ttcgctgttg atttcaatca
     9181 tgatgttgtc aagatcgcaa ccaagcactg ctgccaacac atgctcaaca gtacgcacta
     9241 ttgcatcgcc ttcacggatt gatgtgccgc ggctagtatc aaaaacattc tgtgcaagcg
     9301 ctcgaactat gggtttgccg ggtaaatcca ccctgcagaa tgaatatccg tgattgggtg
     9361 ctgctggtaa aaatcgcatg gtcgtcatac atcctgtatg aattcccttc ccctggattt
     9421 caatgggttc tctgattgtc ttttgtctgc tcattgcatt ttaatcagac aaaagtaaca
     9481 atttatttct tctccaaagc attgactctg aatgcaagtt catcaagctt cttaaaaact
     9541 gcataaatcc tgaaaaaacg agtcgctgaa accgccggag ctcccattac agctgaatga
     9601 tcggaaagat tatccataat acccgattga gccgcaaaac gacaatagct gccaattttc
     9661 aaatgcccgg caaaaccaac ctgaccgcca acctgattgt gatcgccgat ttttgttgat
     9721 ccggcaactc cggcctgggc agcaaaaaca ttgtgctgac ctatctcaca attatgtgca
     9781 accataatca ggttgtcgag tttcgttcct tttcccactc gggtcgatcc catggtagct
     9841 ctgtcaatcg ttgtgttggc gccaacttca acatcttctt caataatcac attgcccaat
     9901 tgcggaattt tgctgaaatg accctgcgca tcaggtgcaa aaccaaatcc atcggtaccg
     9961 ataaccgcac cgggattgag aatacaacgg tctcctatct cgcagtcatg cataattttt
    10021 acacccggat acaaaatgac gtcattaccg attttaacat tctcacctac aaaaacctga
    10081 ggatgaatct gaacattttc accaatcaca gctccggatt gaattacagc aaattcagcc
    10141 accagacatg tgtccggaat cagtaccccc ggtgccacaa aagcacgttc cgagatacct
    10201 ttgtttctgg gcttctgcga ttcggccaaa gccatcagtt ttgcaaacgc ttcgtatgga
    10261 ttacttacac gtatcaaagc cgtattcact tttttcgaag gcacaaaatc ctccgctaca
    10321 atcactgccg aggcttctgt tgaataaata tattgttcat aagccatgtt ggccaaaaaa
    10381 caaaggtctc cggctttgcc ctgttcaatt tttgcaaaat tgctaagttt cgcggcggcg
    10441 tctccatcaa ctttcccccc gatgtaagat gcgacctgcg ctgcggtaaa ttccatcata
    10501 ttcttacaaa tagactgcta aggtaagata aaaattttat ttgggagaaa tcataacaga
    10561 tatggtctcc ggcaacatat tcaaaaatga atatatgtaa attttgtcat tatcgggatt
    10621 ttaatttttt ttatatttgc ctgaaacaaa aaaaagcgat ccaacctttc ggcggaccac
    10681 tttaaatgtt ttcacaacgg aataatcctt attgtgattt gcttagttct gcgacaaaga
    10741 taaaaagaat ttttaaaaat acaacatatg aaaaaaattt tgggattgga tttggggaca
    10801 aatagtattg ggtgggcttt ggtgaatgaa gcggagactg ctgacgaaaa gtcatccatc
    10861 ataaaacttg gcgtaagggt taatcctctc accgtggatg aacaacaaaa ttttgaaaaa
    10921 ggaaaaagta tcaccacaaa tgctgacaga acactaaaac gcagcatgag aagaaacctt
    10981 caaagataca agctaagaag agaaaacctt atttcaattc ttactgaaca caatttaatt
    11041 tctgaaagca caattctttc ggaaaatggt aattcaacga cctttgaaac atatcgtctt
    11101 cgagctaaag ccgctactga agaaatatct ttggaacaat ttgccagggt acttatcaca
    11161 ataaacaaaa aacgtggata taaaagtagt cgtaaagcta aagggacaga tgaaggagaa
    11221 cttattgacg gaatggaagt agcaaaaaag atgtatgagg aaaaccttac tcccggacaa
    11281 ctttgcctga aaattttaga aaccggtaaa agaaatcttc ctgtctttta cagatcagat
    11341 cttcaatctg aatttgattt aatttggaat aagcaaaaag aattttatac tgatatttta
    11401 acaaacgaac taaaggagtc actgaaaggc aaaaacgaaa aacaaacatg ggctatttgt
    11461 caaaaccctt tcaagattgt tggcatcaag cgggcaacaa aaggagaaga attaaaaaaa
    11521 gaaaacttct cgtggcgtgt aaaagcgata aaagaaaaaa ttgacctgga agaattagca
    11581 gttgtgctgc aaaagattaa tggtcagatt aagagttcaa gtggatactt aggagaaata
    11641 agcgatcgta gcaaagaatt gtattttaca aaacaaacag taggtcaata tcaaatggct
    11701 attttggcaa aaaaccccaa tgccagctta agaaacatgg ttttttacag acaagactac
    11761 ctggatgaat ttgaaaaaat ctgggaaacg cagtctcagt atcacaaaga acttactaca
    11821 gaattaaaaa aagaaatccg cgatgttgta atcttttttc agcgcagatt aaaaagccaa
    11881 aaaggattga tcagtttttg tgaacttgaa aaccagcaaa aaagtgtgat tgttgatggg
    11941 aagcataaaa ccattactat tggtcggaaa gtttgtccaa aatcatctcc gctgtttcag
    12001 gaatttaaaa tatggcaaat tctaaataat ttagaaataa caaacaaaat aagcggcgaa
    12061 aaaatactat tggaaaaaga agaaaaggac atcctgtttc tcgaactctc aataaaagaa
    12121 aaactaagca aagatgaagt attaaaatta ttgttcaata attataagga gcttgatcta
    12181 aactataaat cagttgaggg caacaaaacg caggcagccc tgtttaccgc atatcaacag
    12241 attattgaag caagtgggca tggagaaaat gatttctcaa aaatgaaata tgccgacgtt
    12301 attgaaattg ttgaaggagt attcacatca ctcggtttca atacagaaat attaaagttt
    12361 aattctgatc ttgctgtaaa cgagttagaa aaacagcctc attttcaatt atggcatttg
    12421 ctgtattcat acgaaggaga taattccaac acaggaacac aaaaactcat tgaaaaaatc
    12481 agtgcaaaat ttggatttga caaagagtat gcttcaataa tttcgaaaat ttccttccag
    12541 gatgattacg gaagtctgag cacaaaagcc attagtaaga tattacccta tttgaaagat
    12601 ggtgatgaat acagtaaggc gtgttttcat gccggttata atcattccaa aagctcactg
    12661 acacgcgaag aaattgaaaa taaaatactt aaagacaaac ttgacattct taagaaaaat
    12721 agcttgagaa atcctgtggt tgaaaaaatt cttaatcaga tgatcaacgt tgtcaatgct
    12781 gttgttgaaa catttggcaa gcctgacgaa atacgcattg agctggctcg tgagcttaag
    12841 aaaagtgcca aagaacgtga agatatgacc aaagccatta atgaatcatc tgcagcacac
    12901 gaaaaaatca aactattact gcaaaaagaa tttggactca gtcatgtttc aaaaaccgac
    12961 atcattcggt ataagctata cagtgaactt gaaccaagag gatttaaaac actttattca
    13021 aacacgtata tttcgcctga aaaacttttc tcaaaagaat ttgatattga gcatataatc
    13081 ccccaatcaa gacttttcga cgattcattt tccaacaaaa ccattgaatg cagaagtgcg
    13141 aatattgaga aaggaaacga aactgccttc gattatattg ctgccaaata tgatgacaac
    13201 tatcttcagg attatttatc caatattgaa gtactcttca aaaatggcaa tatctcgaaa
    13261 gcaaaataca acaagcttaa aatgcgcgga aaggacatac ccacagattt tatcaaccgc
    13321 gacctgagag atactcaata tattgccagg aaagcaaaaa gcattttgga ggatctggtc
    13381 cgcaatgtta tttcaacctc cggatcaatt acagacagac ttcgcgaaga ttggcaattg
    13441 attgatgtta tgcaggaatt aaactgggat aaatacgaca aactatgtct gaccgaaatt
    13501 attgaagggc gtgacggaca aagaatcagg cgcattaaag aatggacaaa acgcaatgat
    13561 cacaggcacc acgccatgga tgcgcttaca attgccttta caaaacctag ctatattcaa
    13621 tatttgaata atctgaatgc ccggagtgat aaatcaagtt caatatacgg aatcgaacaa
    13681 aaagaactat ttcgtgacaa gttgggaaaa ctacgtttta aaccacctat ggaaattgat
    13741 ttattccgaa ccgaatcaaa aaaacattta caaaacacac ttataagcat aaaagcaaaa
    13801 aacaaagtag ttacttcaaa tatcaatatc actaaaaaac caggacctga taataaaaga
    13861 aaacaattaa cacccagagg tcaattacac aacgaaacag tgtatggtca aattcatcag
    13921 tatgtaacaa aagaagaaaa aattggtgct cagttttcag ttgaatatgt tcaacatgtt
    13981 gcaaaaaaaa cataccgcga tgcattatta atgagacttt cagaaaacaa caacgatcct
    14041 aaatcagcat ttaccggaaa aaattcaatg agcaaaaatc cgatttttat tgatgaagcg
    14101 cattctatga ctgtgcccga aaaagtaaaa cttgtgttca aagagcccgt atacacaatc
    14161 agaaaagaaa tttctgctga tctgaaaatc gagaaagtga ttgatgtaaa aatcagaaat
    14221 attcttgaag cacggcttaa agaattcaac aatgatgcaa aaaaggcctt ttcaaatctt
    14281 gatgaaaatc cgatatggct caataaagaa aaaggaattt cgattaaacg agtaacaatt
    14341 acaggagtta gtaacgccat tcctttgcat gataaaaaag atcatcatgg aaatattatt
    14401 actgatgcac aaggaagaaa gcaagctgtg gattttgtga gcacaagtaa taaccatcat
    14461 gttgccttat acaaggacca aaacggaaat ttacaggaaa aagttgtctc atttttcgag
    14521 gcaacaacca gagccaatca gggactacca attattgaca aagaatacaa caatacggaa
    14581 ggttggaaat ttcttttcag tatgaaacaa aatgaatact ttgtttttcc taatgagagt
    14641 tcaggatttg taccatctga aatagatttg cttaatcctg agaattattt ccttattagt
    14701 cctaatttgt acagagttca aaagtttgga agtttgcttt ctggattttg gttccggcac
    14761 cacttggaaa ccaatgtaga tgcaatgaaa gaactaaagg gaataacata taaagtaatt
    14821 caaagcgcaa acaatttaaa caatttaata aaggttcgtc taaatcacat tgggcaaatt
    14881 gtttcagttg gagaatatta aaatcacaac cttttgcaaa acaatccgtt aaaagaataa
    14941 atgttctttt aatgattaaa aa
//
LOCUS       DAOPKE010000066        14788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4262_length_14788_cov_10.631643, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000066 DAOPKE010000000
VERSION     DAOPKE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4262_length_14788_cov_10.631643"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PKY63_08965"
     CDS             <1..908
                     /locus_tag="PKY63_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00783.1"
                     /translation="IMSFYNDSVPNLRNLSPEDAEHIIEMGTSDNYGWANSIYDIYAL
                     GYHYETNGVKYIGWGRLNAFYSIKHIDTSLVYFRHIDTTLVLSTSLFGAVQNFSTINP
                     SESFNGYYTSSHFSTATAYPVETVFNLQPPSNVEIDTVWVRNSSSNLWGQPHQSASGW
                     QFTPEDSVMLTYFDGFTAKTTGYVYLIGNYAPLSIGDTIHIGLSVYGHYTDTEGFIET
                     ENATDFSVFPNPASNDVTVVLQQPDSAAEIRISDINGRIFSRIQLSGGLQTILINTNE
                     YPGGLYLIQYINRNTTLTKKLIVSH"
     gene            921..2639
                     /locus_tag="PKY63_08970"
     CDS             921..2639
                     /locus_tag="PKY63_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00784.1"
                     /translation="MKKIFSTIIISLNCLVSFSQTFEWKRLSDDIVDSVYQQAVFNTN
                     DIVLKNQNYYFAYTIFPYESEWGGSSVFRAGYLYPTILSQDNQVIMADTLYLDSIFFS
                     QNIATTFIQNDTFRVIINYPVLDSATYCNNTILRKGIPAPKCKGFLMINYSLTGGFLG
                     YDSLALPPAVEHLANLSEAASNCALFIKDLLVNSQQEVFIHLGYNLRSGSAGVFPLDT
                     ASFIVKYNSDLSVSNKVIPVLLKSKDSDFPMSLKIFNENLYLLGSISDSVIVDNMLFI
                     SDTTAGYKTFAVEFDRNLYSLKSKTLPGSYYSFIVDQNNNIYLSGYYNYPLSIDGRVI
                     IPTIYADPDTFYVSDAIIMKLKPDWTIENYINILDGCGSPKMYNNGERIFMKDLRNCG
                     QLLLQNGDTIDNPVSSQTGCNNLILINNSTLIPFWDYNFPLDNTVMNIYFNSDNMLLN
                     FSAYYYYLAGFGDTDTLFLSGYYYPVVLAQISITGLGLEEMSGSEAAPLVVYPNPVSD
                     QLFVKLPENDDFIVFVFDLNGKLQIRKQLSGSENSLNVSGLNPGLYIVHAQNEKQILT
                     GKFLKE"
     gene            complement(2724..3296)
                     /locus_tag="PKY63_08975"
     CDS             complement(2724..3296)
                     /locus_tag="PKY63_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00785.1"
                     /translation="MEVYTSKFNKEKTPVLHSRWLAVSGDKTSANEAIAHFGYPKKTM
                     IRNFNEAFSGMSESIFFDFTNDPYPEKEQPYTGSAKVSISVSPEFSDPAGKELFVLLT
                     TQSIFDGLKLRNDYKNYYSRYVFLPAGTQSVTFRNMHPGTYYLYAFADMNGDKLHLKG
                     DLISSTTDNILVVTENSSASASVIIDMVIP"
     gene            complement(3342..3611)
                     /locus_tag="PKY63_08980"
     CDS             complement(3342..3611)
                     /locus_tag="PKY63_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00786.1"
                     /translation="MISTTSAGSFPEWAVDFSPVSVGQISQFSLLDSNTVNNTSFFIV
                     RHKNELKVAMRTQGCFKNSCCDTYEVIDSVNEEKGYYRFADFQGG"
     gene            3933..4580
                     /locus_tag="PKY63_08985"
     CDS             3933..4580
                     /locus_tag="PKY63_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF00787.1"
                     /translation="MKINLALLFLMIILVSCKKDPKTISISGTVTDPYQNIPVAGTKV
                     TLQANGVVEGVYNSSYVTLGIATTNAAGQFEFIIEESAYDSFRFSFLKDGYYSLQEIV
                     SAGSISPEEPYDGAFDFSSEATVKVHIKNETPFDDNDKLIARFENNPISSFDCCSSTP
                     VTYSGMSIDTTFYCKSVGGFDLKLFKSLLKNNVITYDTDTIHTIPCDTTEVELLY"
     gene            complement(4592..6892)
                     /locus_tag="PKY63_08990"
     CDS             complement(4592..6892)
                     /locus_tag="PKY63_08990"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00788.1"
                     /translation="MNSVDLSNYLSQIENTPPNTVLNYGLKINSTEPVFAYYEVASTY
                     CNCNPEIFALKGKNALGTEFYIPMQHFLDNGSAYFPTPYSSFDIIATESNTTIQINPS
                     NDVVGHVAGVPFIITLNKGETFSVTAASNSASLHLQGSYVVSDKNIAITMKDDLLNGA
                     PYGGCADLGGDQIIPVTMIGNEYIAVRGFLNPPYDKIFLLATQNSTQISVNGALFTTL
                     NAGQSVQYDIGANPAAYFQSSAPIYVTQLSGYGCEIGLSVLPSVECTGSNSVSFARSS
                     TQDLQVTLLVQSTGINNFLFNDIPGIINASSFSNVPGTNGLWQYALLSFDITQVPVGG
                     IGKISNTSDVFHLGLIHGDPTGGCRFGYFSDYARYHFSINASSDSVCVGQSATLSANS
                     LSGASYNWTGPNGFTDNNQSTILSSVSLSSEGWYNVSGNIGSCSVESDSVFLNVNPNP
                     QIIISGDSLLCFNDTFQLYASGASQYSWSGPNNFYANGQTISIQINSDSLTGVYTVVG
                     TDVNGCIDSTETQVSLFVPASITTNSNSPICIGDSIVLSVSGGNSYLWSGPNQFSTTD
                     SVILISDATILNEGVYTIESIDSNNCTTLDTLIISINPLPNVFASNNGPVISGSDAAL
                     YASGGNNYFWFHDNNIFSYSQNPVISPASESDAGYYLVVVTNEFGCSDTTSTNLEIIP
                     FLNFFNVITPNQDGHNDCFIIENGQMIHNSLIIYNRWGKVIFESVDYQNNWSPDGISD
                     GTYFYVFRYGTNLEKEYLGTITVIEK"
     gene            complement(7265..10033)
                     /locus_tag="PKY63_08995"
     CDS             complement(7265..10033)
                     /locus_tag="PKY63_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00789.1"
                     /translation="MNKTYLFLLMTVFIVQTASAQYFIWGTEPNLKWKMRSTPHLNLI
                     SPNSFKADSLFVPYFDSAIVYQTHLLQTHARKLNIVLHPNTLISNGFVAWAPARSELF
                     TVPARDFYPGFWPQQLAWHEMRHLAQYSVAHELLKKRIGWVFGEYLTVGMMGIVVPPW
                     YIEGDATASETVFLNTGRGRSPSFSTPYFAWLNARKKNPDYDRCVLGSDNHYTPDRYL
                     YGYWMVAAGQKSFGAQFWPKVYAETFMKNNFGMFPRSFKQNVPDHTPLRTFHDSTQVW
                     MLNSINAELSATPLTTTIPITESRDYRHYRPIGCDSAGNVVAFSQSIHGEPCFVITGK
                     NRRDIVIKNTNYIHDDAFGFDGRYIYYSEYRPHERWEMVDFSRTLRLDITSGKVENIS
                     VTFKELLPAVSPDGQILATCAYRNDGTYMISVTDLKSSQTLGWAEFPYIEQPLSMAVD
                     NAAKNLYIIQQTVSQRRIIKFGGTTKTAQVLLSSPDKNFSNLTLIGDHLYFNSTMNGN
                     EEIWRISVTGGNPECITNTAFGAAFPVSDGKTGLYFSEMTAHGYQVRHISQITPAEPV
                     SRWEFMYEMADSLSMQTKTLCDSINTTNAEISDAKPYSKDKHLINIHSWGPLSIDPSA
                     GTINPGIQFDSQNMLSTLAGSVFSGYNLSTATIESGVRASYYGWYPVLTLGYTNYLPF
                     VDGAVNYSYNDLNLSASLPLTYSKNAWSFHITPQLETRPWCAIDTGDGMMGYSRYAGF
                     LSLSAYKYTNNRNLYPKLGAFFGGGALWQDFDNHQGTLLAGIGGMWLPGVFRHDGFRI
                     RGGYELRDTLALPFSTMMSAPRCFNLIENPVQSFVSAEYACPLLYPDVHLGSLIFLKR
                     INGMISYDYGVTKNDLNISSVGIHLMADVHFLRLYAPVRLVFSEYYMQPANKWDFQFG
                     LSYDLYAY"
     gene            complement(10141..13089)
                     /locus_tag="PKY63_09000"
     CDS             complement(10141..13089)
                     /locus_tag="PKY63_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPF00790.1"
                     /translation="MKTIALLLTFLSIIGLASASYAQKDSNPGSGTQSSTGHFKYETV
                     PGDPLNARIYTLDNGLKVYMTVYKNAPRIQTYIAVAAGSKNDPHETTGLAHYFEHMMF
                     KGTPHFGTSDFAKESVLIARVDSLFEIYRTLTDEKERAAMYKIIDSVSFAASKYAIPN
                     EYDKLMVSIGAEGTNAYTSLEQTVYIENIPSNQLENWAWIQSDRFANPVLRGFHTELE
                     TIYEEKNMTMTSDSRKVFTALLEGVFPTHEYGTQTTIGTQEHIKNPSMKNIRMFHSQY
                     YVPNNMAIALSGDFNPDEAIKIIDKYFGSMKAGNVPPFTPAVEKPIANPVEKTVLGPD
                     AANLMIGFRFPGAGSDDALKLELLDMILSNSAAGMIDLNLTKKQKTLKAGSGCYILKD
                     YSIQLLYGTPKEGQTLQEVRDLLLQQIEAIKKGEFEDWLLPAIINDLKLKQVKSFEDN
                     DSRAHAFVDAFVTGIPWNQYITKIDRMAKFTKQDIVDFANANFKNNYVVVYKETGKDE
                     SIKKIAKNKLTKLDMNRDMESEMLKQVKASKVNEIEPVYVNFEKDITVSNLKANIPLY
                     YVQNKENETFSMYYVFDMGSNHDKKLPYAIEYLKFIGTDKLTSEELSKKFYSIGCEFN
                     VFNSEDQVYVKLSGLSDNFIEGLDLFENLLANATPNEEAWTNLINDEMKSRADAKKNM
                     QYVFSYLVGYGIYGEKNPMNDQLSEKELKAMTASEAVGIIKSLLSYKHRVLFYGNQNI
                     AELSKILNEKHLAPATLKDIPAERVYTQQATDKNIIYWAHFDTPQSQIIMLNRGEKGF
                     NASLNPTMRLFNEYFGGSMNSVIFQEMREARALAYTAMSYYQTPDKNTDHYYSLSYIA
                     TQYDKMQTAIDAFLELMNAMPESENAFNLAKDGIIKQIRTARTTRDDILWKYENYKKI
                     GVTEDLNKAIYENVPEMTIENLKKFQAMYMKDKPHTYLILGDRKSLPFKTLKKYGKVK
                     EVKIETLFGY"
     gene            complement(13109..14782)
                     /gene="dnaX"
                     /locus_tag="PKY63_09005"
     CDS             complement(13109..14782)
                     /gene="dnaX"
                     /locus_tag="PKY63_09005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPF00791.1"
                     /translation="MDSFVVSARKYRPASFRMVVGQDNIVKTLKNAIINNHLAQAFLF
                     TGPRGVGKTTCARIMAKTINCTNLSPEGEACNECASCLSFNNSASFNIHELDAASNNS
                     VEDIRNLVEQVRVPPQEGKYKIYIIDEVHMLSQAAFNAFLKTLEEPPSYAKFILATTE
                     RHKIIPTILSRCQIYNFKRITVEDIAGYLKYVAESEGVEAESDALHIIAQKADGAMRD
                     ALSLFDQMVTFSGKEISYRSVIENLNILDYDNYFQFVDFFVNGDYRGALVLLNEITSS
                     GFDGQHIISGLSSHIRNLLMARNPATVALLEAGEQMSKRYSEQAARAPEPLLTTSLDM
                     LTSAEFNYRQSNVKSLFLENLMLQLSLILTKLAEQSEAKKKITEAENSNSAKEPETPA
                     YASAPVKKSAEEERKNIPAPNKISIQQKIDELKKNSRSVHQSEPFDRKKMLECWSEYC
                     ESIKTNKINLYSILVNSSIDKTDNDHIAITVDGPMKKAEIEAERDHIIQSMRKKLSND
                     EIMLKIIMTEKEKIHNEISGLAQEKLNEIIEKYPDVSIFVETLNLNPEL"
BASE COUNT         4131 a         2917 c         3134 g         4606 t
ORIGIN      
        1 taattatgag tttttataat gatagtgttc caaatctccg gaacctgagc cccgaggatg
       61 cggaacatat tattgaaatg ggaacatccg ataattatgg ctgggcaaat agcatatatg
      121 acatttatgc tctcggatat cactatgaga caaatggcgt taagtatatc ggctggggca
      181 gattgaatgc gttttattcg ataaagcata ttgacacctc acttgtttat ttcagacata
      241 ttgacacaac ccttgttctt tccacatcgt tgtttggggc tgttcaaaat ttctcaacca
      301 ttaatccttc cgaaagtttt aatggttatt atacatcaag ccatttttcg actgcaacag
      361 cctatcccgt tgaaacggta ttcaacctgc agccaccgtc caatgttgaa attgataccg
      421 tgtgggtgcg caatagcagt tccaaccttt ggggacaacc ccaccaatca gcttctggct
      481 ggcagtttac cccggaagac agcgttatgc tgacctattt tgatggattt actgctaaaa
      541 caaccggcta tgtctatctt atcggaaatt atgctccgct gtctattggc gacaccattc
      601 acatcggtct gagtgtttac ggtcattata ccgatacgga aggatttatt gaaactgaaa
      661 acgcgacaga tttttcagtt tttcctaatc cggcaagcaa cgatgtgaca gttgttttgc
      721 agcagcccga ctcagcagct gaaatccgca tttcggacat caatggcagg attttcagca
      781 gaattcaatt gtccggcgga ttgcagacaa ttttgatcaa tacaaacgaa tatcccggcg
      841 gattatatct tatacaatac attaaccgga atactacact cacaaaaaaa ctgatcgttt
      901 cacactaata actcacagtc atgaaaaaaa tattttcaac cataatcatt tcactcaatt
      961 gtcttgtttc attcagccag acatttgaat ggaaacgact ttcggatgat attgtcgatt
     1021 ccgtttatca gcaagccgtg tttaatacaa atgacattgt tctgaaaaat cagaactact
     1081 attttgctta caccatattc ccatatgaaa gcgaatgggg tggatcaagt gtatttcgcg
     1141 cgggatatct gtatccaacc atactcagcc aggataatca ggttatcatg gccgatacac
     1201 tttatcttga ttctattttc ttcagtcaaa acattgctac aaccttcatt cagaatgaca
     1261 ctttcagagt gatcatcaat tatccggttc tcgattcagc cacatattgc aataatacaa
     1321 ttctcagaaa aggcattccg gcgccgaaat gcaaaggttt tctaatgatc aactattctc
     1381 ttacaggtgg atttttagga tatgatagtc tggctttgcc accggctgtt gaacatcttg
     1441 ccaatttaag cgaagccgct tcaaactgcg cgctattcat taaagatcta ctcgtcaaca
     1501 gtcaacagga agtttttatt catttaggtt ataaccttag aagtggatct gcaggtgtat
     1561 ttccacttga taccgccagt tttattgtga aatacaattc cgatctttcg gttagcaata
     1621 aagttattcc cgttttactt aaaagcaaag attctgattt tccgatgagt ctgaaaatat
     1681 tcaatgaaaa tttgtacctg cttggcagca taagcgattc cgtcattgtg gataatatgc
     1741 tttttatatc ggacactact gccggatata aaacttttgc tgttgaattt gatcggaatt
     1801 tgtattcttt gaaatcaaaa acacttcccg ggtcatatta tagttttatt gttgaccaga
     1861 ataataacat ttatctttca ggttattata attatccatt atctattgac ggaagagtaa
     1921 ttattcctac aatctatgcc gaccctgaca ctttttatgt ttccgatgct attattatga
     1981 aattaaaacc ggattggaca attgaaaatt acattaacat tcttgacggt tgcggatcac
     2041 cgaaaatgta caacaatggt gagcggattt tcatgaagga tctaagaaat tgcgggcaat
     2101 tacttcttca aaatggtgat acaattgata atccggtgag ctctcaaacg ggatgcaata
     2161 atcttattct cattaataat tctacgctga ttccattttg ggattacaat tttcctttgg
     2221 ataatactgt catgaacata tatttcaatt cggataatat gcttcttaat ttcagtgctt
     2281 attattatta tttagcaggg ttcggcgata cggacacatt gttcttaagt ggatattact
     2341 atccggtagt tctggcacaa atcagcatca cagggctcgg ccttgaagaa atgtccggca
     2401 gtgaggcagc gccacttgtt gtttatccga atccggtatc ggatcagcta tttgtgaaac
     2461 tacccgaaaa cgacgatttc attgttttcg tgttcgatct gaatgggaag ctacaaattc
     2521 gaaaacaact ttccggtagt gagaacagtt tgaatgtttc gggcttgaac ccgggcttgt
     2581 atatcgtcca cgcacaaaac gaaaagcaga ttcttaccgg gaaatttttg aaggaataat
     2641 tgagaacttg tttgctgtct gagaaacagc tgccgaaagg ctattttcca atgcttttgt
     2701 tttaaaaatc aatttcatag tctctaagga ataaccatgt caattatcac cgatgcgctc
     2761 gcggaactgt tttctgttac aaccagaatg ttgtccgtgg tcgagcttat cagatcacct
     2821 ttaagatgca gtttgtcgcc gttcatatcg gcaaatgcat acaaataata ggtgccgggg
     2881 tgcatgttcc ggaaggtcac agattgagtg ccggctggta gaaatacgta ccgagagtaa
     2941 taatttttat agtcatttct gagtttcaga ccgtcgaaaa tggactgcgt ggtcagtaga
     3001 acgaatagtt ccttacctgc aggatcgctg aattcagggc tgacagaaat agacactttt
     3061 gctgatcctg tgtagggttg ctctttttct gggtaggggt cgtttgtgaa atcaaagaag
     3121 atactttcgc tcatgccgct gaatgcctcg ttgaaattgc ggatcatggt tttcttcgga
     3181 tatccgaaat gtgctatagc ttcattggcg ctggttttgt cgccgcttac tgccagccat
     3241 ctggaatgca gcaccggagt tttttccttg ttgaatttgc tggtataaac ttccataatc
     3301 atgctatcgc cccggaatgt aaaagctgtt gaagcgcgtt gctaaccgcc ctgaaaatcg
     3361 gcaaaacgat aatatccttt ttcttcgttc accgaatcga taacttcgta ggtgtcgcag
     3421 cagctgtttt tgaagcagcc ctgggtgcgc attgccacct tgagttcatt tttgtggcgg
     3481 acaatgaaga agctggtgtt gtttacagta ttactgtcaa gcagtgaaaa ctgggaaatc
     3541 tgtccgaccg aaacagggct gaaatcgact gcccactcag gaaaacttcc ggctgaggtt
     3601 gtggaaatca ccggtccgtg tcaagttccg gggagacggc tcagtatgcc aaatccttta
     3661 acgggttctg tctgctgttc tgcaattttg ttaccgcgac aggctgacag agctatgctg
     3721 aaggatactg ccaataatat cagaagtatt ttttgcattt gattattttt cattagtaca
     3781 caaaagtatt gtttttccct tattctgatt attaacattg aagatataaa ttagctgaat
     3841 ccaaatgcaa tattctgact tcaggcggtt tactgttctc aaaaatagtt taacgtgtat
     3901 cttccatctg aaataatttg taccttagtg ctatgaaaat aaatctggcg ttattattcc
     3961 tgatgataat tcttgtttca tgcaagaaag accccaagac catttccatc agcggaacgg
     4021 tgaccgatcc atatcagaac attccggtgg cgggcacgaa agtcactttg caggccaatg
     4081 gcgtagttga aggtgtttac aattcgtcgt atgtaacatt gggtattgcc actaccaatg
     4141 ctgcgggtca gtttgagttt attattgaag aaagtgctta cgactcattt cgcttttcat
     4201 ttttgaaaga tggttattat tcgttgcagg aaattgtctc tgctggttct atcagccccg
     4261 aagagccgta tgatggtgca tttgattttt catccgaggc aacggtgaaa gtgcatatta
     4321 aaaatgaaac cccgtttgat gataatgata agctgattgc tcgtttcgaa aacaatccca
     4381 tcagcagttt cgattgctgc agctcaacgc cggtcacata tagcggaatg tcaatcgaca
     4441 ccacattcta ctgtaagtca gttggtggat ttgatctcaa attattcaag tcgcttctga
     4501 aaaacaatgt gattacatac gacaccgaca ccatacacac cattccgtgc gacacgacgg
     4561 aggttgagtt gttgtattga tgacaatttc cttatttttc aataacggtt attgttccta
     4621 aatattcttt ttcgaggttt gtaccatatc taaatacata aaagtatgtg ccatctgaaa
     4681 ttccgtctgg gctccagtta ttttgataat caactgattc aaaaatcact tttccccaac
     4741 gattataaat aatcaaagag ttgtggatca tttggccgtt ttcaataata aaacaatcat
     4801 tatgaccatc ctgatttggt gttattacat tgaaaaaatt caggaagggt attatctcaa
     4861 gattagttga tgtggtatct gaacagccaa attcatttgt aacaacaacc agatagtatc
     4921 ccgcatctga ttctgacgca ggtgaaatca cgggattttg actatatgaa aatatattat
     4981 tgtcgtgaaa ccaaaaataa ttatttccgc ctgaggcata caaagctgca tcggatccgc
     5041 ttatcacagg gccattgttt gaggcaaaga cattgggtaa tggattaatc gaaataatta
     5101 aagtatccag ggttgtacaa ttattagaat cgatggattc gattgtgtaa actccttcgt
     5161 ttagaattgt tgcatcagat atcagtatta cggaatcagt cgttgaaaac tgatttggtc
     5221 cagaccacag atatgagttt ccgccagata cagaaagcac aatgctatca cctatgcaaa
     5281 ttgggctatt ggaatttgta gtaattgaag ccggtacgaa taatgagacc tgtgtttccg
     5341 tgctatcaat gcatccattg acatctgttc caacaacagt atatacgcca gtaagcgaat
     5401 cggagttgat ttgaattgaa attgtctgac cattagcata gaaattattt ggaccagacc
     5461 atgagtattg agatgcacca gaggcatata attgaaaagt atcgttgaaa cagagcagcg
     5521 agtctcccga aatgataatc tgcggatttg gattgacatt aagaaaaacc gagtcagatt
     5581 caacactgca gctcccaatg ttcccagata cgttgtacca tccttcgctt gatagactaa
     5641 cagagctcag gatagtgctt tgattgttgt ctgtaaaacc gtttgggcca gtccaattgt
     5701 atgatgcccc agaaagtgag tttgcagata atgtggcgct ttgtccaaca catacagagt
     5761 cgcttgatgc attaattgaa aagtgatacc tggcatagtc tgagaaatat ccaaacctgc
     5821 atccgcccgt tggatcccca tgaatcaatc ccaaatgaaa cacatctgat gtatttgaaa
     5881 ttttaccgat ccctccaacc ggtacctgag taatatcaaa tgataataac gcatattgcc
     5941 acaaaccatt tgtgccaggt acattggaaa aagagctggc attaataata cctggaatgt
     6001 cgttaaaaag aaaattatta ataccagtgc tttgcaccaa caatgttacc tgcaaatctt
     6061 gtgtgcttga acgtgcaaaa ctgacactat ttgatccggt acattctacc gaaggcagga
     6121 cacttaaacc aatttcacag ccatatcctg aaagttgtgt aacatatatg ggtgctgaag
     6181 actggaaata tgcagctgga ttagctccga tgtcgtattg cacactttgt cctgcattta
     6241 gtgtcgtgaa aagtgctcca tttactgata tttgggttga attctgcgtg gccaacagaa
     6301 atattttatc ataaggagga ttcagaaaac ctctaacagc aatatattcg ttgccaatca
     6361 ttgtaaccgg aataatctga tcccccccta aatcggcgca accgccataa ggagctccat
     6421 ttagaagatc atccttcatt gttattgcaa tgtttttatc tgatactaca tatgatcctt
     6481 gcaggtgcag tgaagcgcta tttgaagcag ctgtaaccga gaatgtttct cctttgttta
     6541 atgtaattat aaagggaacg cctgctacat gccccaccac atcatttgat ggatttattt
     6601 gaatggttgt gttactttct gttgctatga tatcaaaaga tgaatatggt gttgggaaat
     6661 aggccgaacc attatccaga aaatgttgca tgggaatata aaactctgtc cctaatgcgt
     6721 ttttgccttt taatgcaaaa atttccggat tgcaattgca atatgtactt gccacttcat
     6781 aataagcaaa tacgggttca gttgagttta ttttcaaacc ataatttaac actgtatttg
     6841 gtggcgtgtt ttcaatctga cttaaataat tcgacaaatc aacactattc atactgttgg
     6901 ctggaattgc gaccactatt ggagcaaatg tggcattggc cggctgtgaa attgttactg
     6961 ttgcaggatt tgattcagtt gtgattctta aaaatattgg aatatcaaaa ctttgattaa
     7021 cggaaacctc tggcgcggca aaccaaaagg ctgtatcttt ttggcaaaat gagaagatgg
     7081 atatacatga taaaatacat gcagcatata catgtttcat ataaacgagg ttgataatta
     7141 atattcaaaa caattttcaa aaatagtaca attatttaat atgatacata taactcatat
     7201 gcatacaaac cataactcaa aaagggccac gcacaaatga taatttataa tccgccccta
     7261 cccatcaata cgcatacaaa tcataactca gtccgaactg gaaatcccat ttattggcgg
     7321 gctgcatgta atattccgaa aacaccaggc gtaccggcgc atacaagcgc agaaaatgta
     7381 catcggccat caggtgaatt ccgacggatg aaatattcag gtcatttttt gtaacgccgt
     7441 aatcatagct gatcatgcca ttgatgcgtt tcagaaaaat caatgaaccc aggtgtacat
     7501 cgggatagag cagggggcag gcatactctg ctgaaacgaa ggattggaca gggttttcaa
     7561 tcaggttaaa acaacggggc gccgacatca tggtgctaaa cggaagtgca agcgtatcgc
     7621 gcaattcata tccgccgcgg atgcgaaaac catcgtgccg gaaaacgccc ggaagccaca
     7681 ttccgccaat accagcaagc aatgttccct gatgattatc gaagtcctgc cacagtgccc
     7741 caccaccgaa aaacgcaccc aatttgggat acaggtttct gttgtttgtg tatttatatg
     7801 cagacaatga taaaaatccc gcatatctgg aatatcccat catgccatct cctgtgtcaa
     7861 tggcgcacca ggggcgcgtt tcgagctgcg gtgtgatatg gaaagaccag gcattttttg
     7921 aataggtcag tggaagcgaa gccgataaat tcagatcgtt gtaagaataa ttcacggctc
     7981 cgtcaacaaa aggcagataa ttggtatatc ctaaagttag aaccgggtac cagccgtaat
     8041 atgatgcccg cacaccagat tctattgttg ctgttgataa attataacca ctgaaaactg
     8101 aaccggcaag tgtgctgagc atattctgcg aatcgaactg aatgcccgga ttgatggtgc
     8161 cagctgaagg atcgatggac agcggacccc aagaatgtat attgatcaga tgtttgtctt
     8221 tggaataggg ctttgcatct gaaatttcag cgttggttgt attaatggaa tcacacaatg
     8281 tttttgtttg catgctgagc gaatcagcca tttcatacat gaattcccat cttgaaaccg
     8341 gctctgcagg cgttatttga ctgatatgac ggacttgata tccatgtgcg gtcatttcgc
     8401 tgaaatacaa tcctgtcttt ccgtccgaaa ccggaaaagc cgctccaaat gcggtgtttg
     8461 tgatgcattc aggattcccg ccggtcactg aaatacgcca gatctcttca ttgccgttca
     8521 tggttgaatt gaaatacaaa tggtcgccaa tcagtgtaag attgctgaaa tttttatcgg
     8581 gcgatgataa taaaacctga gctgtttttg ttgtaccgcc gaattttatg atccggcgct
     8641 gtgaaacggt ttgctgtatg atatatagat tttttgcggc attatcaaca gccatagaca
     8701 gtggttgttc aatatatgga aattcagccc agcccagtgt ttgcgagctt ttcagatcag
     8761 taacggaaat catgtaggtt ccgtcgttgc ggtatgcaca ggttgccagt atttgtccgt
     8821 cgggactaac agctggcagc aattctttaa atgtgacgga aatattttca acttttcccg
     8881 aagtgatgtc gaggcgcaat gttctcgaaa agtccaccat ttcccatctt tcgtgaggcc
     8941 tgtattccga ataataaata tagcggccgt caaatccgaa agcatcgtca tgtatgtagt
     9001 ttgtgttttt gattactata tcgcgccgat tttttccggt aatgacaaag cagggttcgc
     9061 catgaatcga ttgactgaaa gcaacaacat tgcctgcaga atcgcatcca atgggtctgt
     9121 aatgacgata atccctggat tcagtaattg gaattgttgt tgtcagagga gtggctgata
     9181 gttcagcatt gatagaattc agcatccaga cttgtgttga atcatggaac gtacggagag
     9241 gagtatggtc aggaacattt tgcttgaatg atcgcggaaa cattccgaaa ttatttttca
     9301 taaaagtttc agcatatact ttcggccaga attgcgcacc gaatgatttc tgacctgccg
     9361 ccaccatcca gtaaccatac agatatctgt cgggggtgta atgattgtcg gagccaagaa
     9421 cacatcggtc ataatcagga tttttttttc gtgcgttcag ccaggcaaaa tacggagttg
     9481 agaaagaagg agaacgtccc cggccggtat tcaggaatac tgtttccgaa gcggtggcat
     9541 caccttcgat ataccagggt gggacaacaa tacccatcat tccaaccgtc agatattcac
     9601 caaagaccca tccgatgcgc tttttcaata gttcatgagc cacagaatac tgagccagat
     9661 gccgcatttc gtgccatgcc agttgctgag gccagaagcc aggataaaaa tcacgtgccg
     9721 gcacagtgaa cagctcactt cgtgccggcg cccatgcaac aaacccattt gaaattagtg
     9781 tattggggtg aagaacaata ttgagttttc gggcatgagt ctgcaatagg tgtgtttggt
     9841 aaacgatggc tgaatcaaaa taagggacaa acaaagaatc cgctttaaaa gagtttggcg
     9901 aaatcaggtt cagatgcggt gttgaacgca ttttccattt gagatttggt tcagttcccc
     9961 aaatgaaata ctgtgcagat gcagtttgca caataaacac agtcatcaat aaaaacagat
    10021 aggttttatt catggggcag ataatgattc attgggtgca atattaaaaa aagccgatcg
    10081 atattcaaag ataaaaaaag gcgggcataa gagcccgcct ttatagaaaa attagttggg
    10141 ttaataacca aacagagttt caattttcac ttcttttact tttccgtatt ttttcagtgt
    10201 tttgaaagga agagatttgc ggtcaccaag aatgagataa gtgtgaggtt tatctttcat
    10261 atacatggcc tggaatttct tcagattctc gatagtcatt tcgggtacat tttcataaat
    10321 ggctttgttc agatcttcgg taacaccaat ttttttgtag ttttcatatt tccacaaaat
    10381 gtcgtcgcgt gttgtgcgcg cagttctgat ctgtttaata attccatctt ttgcaaggtt
    10441 aaatgcattt tcagattcag gcatggcatt catcagttca aggaaagcat cgatggctgt
    10501 ctgcattttg tcgtattgcg ttgcaatgta tgaaagactg tagtagtgat cggtattttt
    10561 atcaggagtc tgataatagg acatagccgt atatgccagg gcgcgggctt cgcgcatttc
    10621 ctgaaagata acggagttca tgctgcctcc gaaatattcg ttgaacagtc gcatcgtagg
    10681 attcagagat gcattgaaac ctttttcgcc acgattgagc ataataatct gtgattgagg
    10741 agtatcgaaa tgcgcccagt aaatgatatt tttatctgtc gcctgctgtg tgtacacgcg
    10801 ctctgcaggg atatctttca atgtagcggg agcaagatgt ttttcattca gtatcttgct
    10861 taattcagca atattctgat tgccataaaa aagtactcga tgtttgtagc taagcagcga
    10921 tttgatgatt ccaacagctt cgcttgctgt catggctttg agttcttttt cggaaagctg
    10981 gtcgttcatt gggtttttct cgccatagat gccatatcca actaagtagc tgaatacata
    11041 ttgcatgttt ttctttgcat cggcacgcga tttcatttcg tcgttgatca gattggtcca
    11101 tgcttcttcg ttgggggtgg cattggccag cagattttcg aataaatcaa gtccttcaat
    11161 gaaattatcg cttaatccac tgagttttac atatacctga tcttcagaat tgaaaacatt
    11221 gaattcgcag ccaatgctgt agaatttttt actcagttct tcagatgtta atttgtcggt
    11281 tccgataaat ttcaggtatt cgatggcata gggaagtttt ttatcatgat tgcttcccat
    11341 gtcaaaaaca taatacatgc tgaatgtctc attttcttta ttctgcacat aataaagagg
    11401 aatgtttgcc ttaaggttgg aaacagtaat gtctttttca aaatttacat ataccggttc
    11461 aatttcattt actttcgagg ccttgacttg tttaagcatt tcactttcca tgtcacggtt
    11521 catgtcaagt tttgtgagtt tgttttttgc aattttcttg atggattcat ccttgccggt
    11581 ttccttgtaa acaacaacat agttgttttt gaaattggca ttggcaaaat caacaatatc
    11641 ctgtttggtg aatttggcca ttcggtcaat tttcgtgatg tattgattcc atggaattcc
    11701 ggtaacaaag gcatctacaa aagcatgagc ccttgaatca ttgtcttcga acgatttcac
    11761 ctgtttcagt ttcaggtcgt tgatgatggc aggcaaaagc cagtcttcga attctccttt
    11821 tttgatggct tctatttgtt gaagcagcag atcgcgtact tcctgcagtg tttgcccttc
    11881 tttgggtgtt ccatataaaa gctgaatgga atagtctttc agaatatagc atcccgagcc
    11941 ggctttcagc gtcttttgtt ttttggtgag attcaggtca atcataccag ctgcactgtt
    12001 tgaaagaatc atgtcgagta attcaagttt gagtgcatca tcgctaccgg cacccgggaa
    12061 gcggaatcca atcatgaggt tggctgcgtc gggaccaaga actgtttttt caacagggtt
    12121 tgcaatcggc ttttcaactg ctggtgtgaa tggtggaaca ttgccggctt tcatcgaacc
    12181 aaaatattta tcaataattt tgatggcttc atccggattg aaatcgcctg aaagagcaat
    12241 agccatgttg tttggaacat aatactgcga gtggaacatg cggatgtttt tcatcgaagg
    12301 atttttgata tgttcctgag ttccgatggt tgtttgagtt ccgtattcat gagttgggaa
    12361 tactccttcg agcaaagcag tgaaaacttt gcgggaatcc gaggtcatgg tcatgttttt
    12421 ttcttcgtag attgtctcaa gctctgtatg gaagccgcga agaacaggat ttgcaaaacg
    12481 gtcggattgt atccatgccc agttttcgag ttgattggac ggaatatttt caatgtaaac
    12541 ggtttgttcg agcgaagtat aggcattggt accttcggct ccgattgaaa ccatcagttt
    12601 gtcatattca tttggaatag catattttga tgctgcaaac gaaacactgt caatgatttt
    12661 atacatggca gcgcgttctt tttcatcggt tagtgtgcga tagatttcaa acaatgaatc
    12721 cactctggca atcaaaaccg attcttttgc aaaatccgat gttccgaaat gcggagtgcc
    12781 tttaaacatc atgtgttcaa aatagtgagc cagacccgtg gtttcatgcg ggtcgttttt
    12841 gcttccggct gcaacggcaa tataggtctg aattctcggt gcatttttat acacggtcat
    12901 atatactttc aacccattgt cgagtgtata tatgcgcgca ttgagagggt cgccgggaac
    12961 ggtttcgtat ttgaaatgtc cggtgctgct ttgtgtcccg gatccgggat tggaatcctt
    13021 ttgggcataa cttgccgaag caagaccgat aatgctcaga aaggtcaata ataaggcaat
    13081 cgttttcata tttgttgttt atgttgtttt acaattcggg attcagattc agtgtctcaa
    13141 caaaaattga aacatcgggg tatttttcaa taatctcgtt taacttttcc tgtgcaagtc
    13201 cgctgatctc attgtgaatc ttttcttttt ctgtcatgat tattttaagc atgatttcgt
    13261 cgttgcttag ttttttacgc atcgattgaa tgatgtgatc gcgctcggct tcgatttctg
    13321 cttttttcat cggtccatca acagtgatgg ctatatgatc attgtcggtt ttatcgatgg
    13381 atgaattaac cagaatggaa taaagattta tcttgttggt ttttattgat tcgcaatact
    13441 ctgaccagca ttccagcatt tttttccggt caaatggttc gctttgatga acagacctcg
    13501 aatttttttt cagttcgtca attttttgct gaatactgat tttgtttggt gccggtatat
    13561 ttttacgttc ttcttcggcc gattttttta caggagctga tgcgtaggcc ggggtttctg
    13621 gttcttttgc tgaattggaa ttctcggcct cagttatttt tttttttgct tcgctctgtt
    13681 cggcaagttt ggtcagaatc agacttagtt gtaacatcag gttttcaaga aacagacttt
    13741 ttacattact ttgtctgtag ttgaattcag cactggtaag catatccagt gaagtggtca
    13801 gcaaaggttc gggagcacgg gcagcttgtt cggagtaacg ttttgacatt tgctcgccag
    13861 cttcgagcaa agcaactgtg gcaggatttc tggccatcaa cagatttcgt atgtgactgc
    13921 taagtcctga aatgatatgt tgtccgtcga aaccactcga tgtaatttca ttcaatagaa
    13981 ccaatgcgcc tcggtaatca ccgtttacaa agaaatcaac aaattggaaa taattatcgt
    14041 aatcgagaat attgaggttt tcaatcaccg agcggtaact gatttctttg ccgctgaaag
    14101 taaccatctg atcgaatagt gacagggcat cacgcatggc gccatcggct ttttgtgcga
    14161 tgatgtgaag cgcatccgat tcggcctcta cgccttcgct ttcggctaca tatttaagat
    14221 agccggcaat gtcttcaacg gtaattcgtt tgaaattata aatctgacag cgcgacagaa
    14281 tagttggaat gattttgtgc cgttcggttg ttgccagaat gaatttagcg taagaggggg
    14341 gttcttcgag tgttttcaga aatgcattga atgcagcctg cgacaacatg tgaacctcat
    14401 cgatgatgta tattttatac ttgccttcct gtggtggaac gcgaacctgt tctaccagat
    14461 tgcggatgtc ttcaacggaa ttgttggatg cggcatcgag ttcgtgtata ttgaacgatg
    14521 ctgagttatt gaacgacagg catgaagcac attcgttgca ggcttcccct tcgggcgaaa
    14581 gattggtgca gttgatggtt ttcgccataa tacgtgcaca tgtcgttttg ccaactccac
    14641 ggggacccgt gaataaaaat gcctgtgcca gatgattgtt gatgatggca ttcttcagtg
    14701 tcttgacaat attatcctgt cccaccacca ttctgaatga tgccggccgg tattttcgtg
    14761 cagaaacaac aaaagaatcc atatcaac
//
LOCUS       DAOPKE010000067        14413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4399_length_14413_cov_11.579816, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000067 DAOPKE010000000
VERSION     DAOPKE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4399_length_14413_cov_11.579816"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..186)
                     /locus_tag="PKY63_09010"
                     /pseudo
     CDS             complement(55..186)
                     /locus_tag="PKY63_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
     gene            336..1535
                     /locus_tag="PKY63_09015"
     CDS             336..1535
                     /locus_tag="PKY63_09015"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HPF00792.1"
                     /translation="MDKSTHFFGQSVFGQLISMISLQIIGKAVSKTNSDYYTKRFSTK
                     DHLISMVFGVFAKCSSLREVSGAMLGLSGKTKHFQLESIPYRSTLSDANAKRNCEVFG
                     LIYNMLLREYGQFISDSRIKDVIKKQIEIFDSTTISLFKDILACVGRKPQNGKEKGGI
                     KMHTVINVDEVVPKMVWFTSAATHDHVLLDKLKMNSNTIYVFDKGYNDYKAFEKFCLN
                     NTGFVTRIKDNAVYDVTSVDEIDDNIHSGVLEDTIIEIVVKDKNGDRKLRLRKVRFYD
                     RALNREFEFITNLFDIRPDLIAAIYKLRWQIELLFKQLKQNFPLRYFLGDNENAIKIQ
                     IYCALIVNLLMTVIQKQLKRKWAFSNLVSFCKIHLFNYIHLMRFLENPEKDWQKEQYK
                     SEQMALF"
     gene            complement(2108..2680)
                     /locus_tag="PKY63_09020"
     CDS             complement(2108..2680)
                     /locus_tag="PKY63_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPF00793.1"
                     /translation="MEYIVIVISAIFVSNIIFAQFLGVCPFLGVSKKISTAVGMGGAV
                     VFVMTLATLVTYLIQVYVLEVFSLQYLQTIVFILVIASLVQMVEIILKKMSPPLYQAL
                     GIFLPLITTNCAVLGVAIMVITKEFNLGEALVFAVSTAVGFTLALVLFAGIREQLDLM
                     GVPKGMRGVPIALVVAGILSLAFMGFANLV"
     gene            complement(2693..3280)
                     /locus_tag="PKY63_09025"
     CDS             complement(2693..3280)
                     /locus_tag="PKY63_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPF00794.1"
                     /translation="MNQWTNFTKGFIKENPVLVLLLGMCPTLGVTTSAINGIGMGLAT
                     TFVLIMSNVIISLIKNFIPSSVRIPSFIVVIASFVTVVDLSMKAYIPDLHAQLGLFIP
                     LIVVNCIVLGRAEAFASKNNVFSSFIDGLSMGLGFTFALGLLGSIREILGGGSIFNYK
                     FIPADMDGILVFVLAPGAFIALGYLIALVNSFKKK"
     gene            complement(3277..3846)
                     /locus_tag="PKY63_09030"
     CDS             complement(3277..3846)
                     /locus_tag="PKY63_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPF00795.1"
                     /translation="MAKKKNSLFNMTSTLFIISAVAAFALAGVYSLTKGPIELSEKKK
                     IETAIKEVIPEFETLVQGQVKCADSDDSLTVYYGVKGTDTTGIAVESFTKNGFSGLIT
                     MMVGFLPDGTINNTKVLTHAETPGLGTKMMEDKFKNQFMQKNPSSYKLAVKKDGGDVD
                     AITASTITSRAFCDAVSRAHQSIWKGGQE"
     gene            complement(3839..4828)
                     /locus_tag="PKY63_09035"
     CDS             complement(3839..4828)
                     /locus_tag="PKY63_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPF00796.1"
                     /translation="MNMLTISGSPHLHARGDAGKVMRLVILAMLPALLGSFYLFGLGA
                     VKVFLVSVLACVLFEYLIQRFLLKDKSTIGDGSAVVTGILLGFNVPSNLPVWILIVGA
                     LVAIGIGKMSFGGLGKNPFNPALVGRVFLLISFPVQMTSWPKPIESASQLTDVVTGPT
                     PLALLKESDGFTAAMAKPEMPDYMNMLLGNMGGSVGEVSALLLLIGAAVLLITRVISW
                     HIPVSYILGAVLFSGALWLYDPSYYADPLFHLITGGMMLGVFFMATDMVTSPMSPLGM
                     LIFGFMCGFLTILIRVFGAYPEGVSFAILIMNAFVPLLNKIKPKRFGEVVKNG"
     gene            complement(4844..6175)
                     /gene="rsxC"
                     /locus_tag="PKY63_09040"
     CDS             complement(4844..6175)
                     /gene="rsxC"
                     /locus_tag="PKY63_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPF00797.1"
                     /translation="MLKTFKMGGVHPEECKLSEHSAIETLEAPDTVYVMLNQNLGAPS
                     VPVVNKGDKVKTGQLIAKGEAFISASIHSPVSGTVEKVDSWPDASGYRKPVIIIKKDG
                     DEWNDGIDTSKDLVKEIKASKAEIIDIIKNSGIVGLGGAAFPTHVKLMVPQGKKAEYL
                     IINAVECEPYLTADHRLMLEKGEEILIGVQILMKALEVTKAIIGIENNKPDAIAHLTK
                     LSASYPGISVQGLKVKYPQGAEKQLIKAAINREVPSGKLPIEVGCVVQNVGTTFAVYE
                     AVQKNKPLIERVVTVTGKKVSKPSNFMVRIGTPVSVLIDKCGGIPEGTGKMINGGPMM
                     GKAFASTDISVTKGTSGILLMDEKEAKRVKEYNCIRCGRCTTVCPMGLEPYLLGLAGE
                     NQNWEIAENNRVMDCMECGSCQFTCPSGRPLLDSIRLGKNKVGQIIRKRNN"
     gene            complement(6194..7057)
                     /locus_tag="PKY63_09045"
     CDS             complement(6194..7057)
                     /locus_tag="PKY63_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPF00798.1"
                     /translation="MDPILLAVIVLGSVALVFAAIIFGVEKKFMVVEDPRIDLVVEKL
                     PGANCGGCGFAGCRNFAEAVVKNESLDGLFCPVGGNATSAEVAAVMGLTAAEKAPQVA
                     VVRCQGSFANSPARSDYAGISSCAYAHSLYAGPGGCPNGCLGLGDCVASCAFDAMYMD
                     KTTGLPVVIEDNCTSCGACVKACPRSIIELRNKGPKSKRIFVSCVNTEKGAVAKKNCV
                     TACIGCGKCVKACPHDAITLENNLAYINFEKCKLCRKCVAECPTGAILEINFPARKIE
                     SAPAGEDQITQ"
     gene            complement(7289..8248)
                     /gene="nusB"
                     /locus_tag="PKY63_09050"
     CDS             complement(7289..8248)
                     /gene="nusB"
                     /locus_tag="PKY63_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPF00799.1"
                     /translation="MLSRHIIRSKTLQAVYSYVVGGNTDLEKGRRALVDSVDDMRDLI
                     FIQISALLELRDIAERKIEENRHKHVPTKEDINPNLTFVQNPLLMHLRDNAHVQNAIN
                     GLRVNWADMNDIFRKIYKEVLDWSEYQNYTNKGEHDYASHRQFIVKLFKKFIAFDSNL
                     RDRYEEKNIHWGDDSVLTGLMVHAWLKSYDPEKESTTVFPALLKPSEGIGDDDDRTFM
                     IKLFDKTILNLSAYDDLIEKHLKNWDIERIIVIDMLVIKLALTEIIHFDNIPVRASMN
                     EYIELSKEFGTLKSNSFVNGLLDKIISDLKAEGKIKKSGRGLV"
     gene            complement(8417..10267)
                     /locus_tag="PKY63_09055"
     CDS             complement(8417..10267)
                     /locus_tag="PKY63_09055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPF00800.1"
                     /translation="MAEQYNEESIKSLAWNEHIRLRPGMYIGKLGNGASHDDGIYVLI
                     KEILDNAIDEFIMGYGKKVDVSIKGNEVTVRDYGRGIPLGSVVDVVSKINTGAKYDSE
                     VFKKSVGLNGVGTKAVNALASAFRIESFREGKKVTADFSGGNLIKQAKDTVGANERGT
                     RVSWTPDETIFGEYEYFNEHIERLLWNYAYLNKGLCINFNGEKYESKNGLLDLLERNI
                     DGETIYPVIHMEEGDLEFAFTHSHKNYGEEIYSFVNGQHTTQGGTHVAAFKEALVDTV
                     RKFYKKDFEASDIRGSIVAAISIRVHEPVFESQTKTKLGSQYMEPNGQTIRNYVGDII
                     RRNLDNYLHIHSETSDSLLQKILANEKERKELANIKKIAKESFKKVSLHNKKLRDCKL
                     HFNEDKDERCMQTTLFITEGDSASGSITKARDPYTQAVFSLKGKPLNTYGLSKRIVYE
                     NEEFNLLQAAINLEEGIENIRYNNVVVATDADVDGMHIRLLLLTFFLQFYPDLVRSGH
                     LYILQTPLFRVRNKQKTIYCYSDQERVSAIQELGPKPEITRFKGLGEISPDEFRQLIG
                     PKMRLENVLLKKETGLAEILKFYMGKNTPDRQSFIIENLRIEEDIPEPAV"
     gene            complement(10340..10579)
                     /locus_tag="PKY63_09060"
     CDS             complement(10340..10579)
                     /locus_tag="PKY63_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00801.1"
                     /translation="MISSHSWTYCDKPFIHGEYGSGTNQGYYMCDNDASWIRDLWIGS
                     FTGICGSALLWDNMHRRDLWPHFGRLRAYSSCFIF"
     gene            complement(10576..11217)
                     /locus_tag="PKY63_09065"
     CDS             complement(10576..11217)
                     /locus_tag="PKY63_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00802.1"
                     /translation="MKWLFLLSALIPVLSGNAQTDSVKKKEFVAELFFQMNPSSRDGF
                     SLNQNSNFSVETGAKIRVKKSMIMKFEDNLFICLAFKKNSINWHYVNEIFTASYLNFG
                     VGYSIDFRLSKVFSLSIVDELLYNHIINATETNPNNRYFIDYTDRALQNKLGLAIYWN
                     LSKNCSFGISPKIAPCIRLSPRKYGSISHMEYYVSVLDDGWPFYLGTSLMIKL"
     gene            complement(11222..11779)
                     /locus_tag="PKY63_09070"
     CDS             complement(11222..11779)
                     /locus_tag="PKY63_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00803.1"
                     /translation="MKMNCDNDASWIRDLWIGSFTGICGSALMWDNMHKRELWPHFGR
                     LRAFVAGYDFDESNGDQKGWIPYHDFDEMHYENWQRKPGIVDLFYLRSPDKLNAVGVL
                     ANRTFNFYTQWTDPLIYPNPGNGCFLLGFDPQNAALELTDENGKDIPFEIQGQTLCCN
                     APDGIYFIRFLLDGHLQIIPVIIKK"
     gene            complement(11776..12171)
                     /locus_tag="PKY63_09075"
     CDS             complement(11776..12171)
                     /locus_tag="PKY63_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00804.1"
                     /translation="MGDKTVFVGNWQWVHSIGKYPDPGGGGVISHQIFPSDINKSFSM
                     EFRENGKYLLKDGDDVIHREKAKFATWNPVDTAEIFYFSIVINPDTRLEGKIFGDTVI
                     TYQYSSPFYFPVFDNDGIYSSYTNIFVRE"
     gene            complement(12251..12715)
                     /locus_tag="PKY63_09080"
     CDS             complement(12251..12715)
                     /locus_tag="PKY63_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00805.1"
                     /translation="MKNSSRIKNSIIFIGLILGLYSCSEYDLIGDKAIFIGNWKWVYS
                     IEEVHDNGLIYYRTLTPESENTNYSMEFRENGKYKLFENDDVWYKDKSKFLDWQSIDS
                     GTCFYFWFYIDKDQQFYGKILEDSIVTSNYFPPFETDHGYSYSTYKSYFVRE"
     gene            complement(12693..>14413)
                     /locus_tag="PKY63_09085"
     CDS             complement(12693..>14413)
                     /locus_tag="PKY63_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00806.1"
                     /translation="EQICNYYNRMHIGWEVDNIVEKSEQEGLFIHFNTAIQYYFLKPD
                     HLLWDWSAYGEAGLPEECDNNSDHGYCYNSDLGLTDPKLFFSDYYAKDYYQKRLRYMI
                     ARWGYSTNISAFETINEINGTCGTKEVYYNPDDSTCNVVPGSGYSPYNQEVLFRQNVN
                     EWQSSMLNSMTAYMGHNQHLTSVNYLTDGPNISDDTTYYLNSLDIITQQNYRDFPDIY
                     KSTVDIVNDLHDNSNASSFGWTYCDKPFIHGEYGSGTNQGYYMCDNDASWIRDLWIGS
                     FTGICGSALLWDNQHKRELWPHFGRLRSFVQGIDFDNSGDSQHKGWTNENDLRGDNLA
                     EMMCLRNWEDNHKQAIGVIANRTYNYYTQAEGGECQADIPMVPYNSALNIGPVGNSAN
                     YWELLMIPKMGSFVRYNIEFYNVMTGQFIQTFEKVRSDVDGELFLEYPQLTGNAKCPI
                     VLFKIYKWKDRSMLECLDTLNRYSDESNSLMNMGNATTVDNSVVKSGIKVFPNPANDI
                     LNITYSGIQAESIVQIFSTEGRLILLLTMTGQNLQIPLDQIVPGIYIIVVQNEEDSYH
                     EKFIKN"
BASE COUNT         4344 a         3155 c         2790 g         4124 t
ORIGIN      
        1 tattttataa atatattaac atcgcaaacg tgatttattc acggttattt gccatcacag
       61 cctattcaat ttcaacagca caaaaaatcg cagcaaatgt atctgccgtt ttattcttga
      121 tggttgtttg accattctgt aggcccattc aagattatgt ttcacccacc atgcgggagc
      181 gcagcgcgcg aaaaaataaa aaaaacgccc ttccaataat tcagaagagc gctgtgtttt
      241 aaaaatccta aaacactatt gtccgataaa aaataaagtc gaagcataag cctcgacttc
      301 tttttgtagt tttggttcgc taaaactaat ctacaatgga taaaagtacg catttttttg
      361 gacaatcggt tttcggacag ctgatttcta tgatctctct tcaaattatc ggaaaagcgg
      421 tgtccaaaac gaattcggat tactatacaa aacgatttag caccaaagat catcttatta
      481 gcatggtgtt tggcgtgttc gcaaagtgta gttcgttgcg cgaggtatcc ggcgccatgc
      541 ttggtttgtc tggaaagaca aaacattttc aacttgagag tattccttat cgaagtacat
      601 tgtccgatgc caatgcaaaa aggaactgcg aagtatttgg actcatatac aacatgctgt
      661 tgcgcgaata tgggcagttt atatcggaca gccgaatcaa agatgtgata aaaaagcaga
      721 tagagatttt cgacagcacg accataagcc tttttaagga tattttggcc tgtgttggac
      781 gaaagccaca aaacgggaaa gaaaaaggcg gaatcaaaat gcatacggtc attaatgtag
      841 atgaagttgt tccaaagatg gtctggttta caagtgcagc cacgcatgat catgtgctgc
      901 tcgataagtt gaaaatgaat tcgaatacca tttatgtttt tgacaaaggg tataacgact
      961 acaaagcttt tgaaaaattt tgtttgaata acaccggctt tgttacaaga ataaaggata
     1021 atgcagtata cgatgtaaca tcggtggatg aaattgatga caatattcat tccggtgttc
     1081 ttgaggacac aattattgaa attgtggtga aagataaaaa cggagacaga aagctacgct
     1141 tgcgcaaggt taggttttat gacagagccc tcaatcgtga atttgaattt attaccaacc
     1201 tgttcgatat aaggccagat cttattgcag ccatctacaa actgcgctgg caaattgaat
     1261 tactattcaa gcaactcaag caaaattttc cgttgcgata ttttcttggc gacaatgaaa
     1321 atgcaataaa aatccagata tactgcgccc tcatagttaa tctccttatg actgttattc
     1381 aaaagcagct caaaagaaaa tgggctttct caaatcttgt cagcttttgc aaaattcatc
     1441 tgttcaacta cattcatttg atgcgatttc ttgaaaatcc agaaaaagac tggcaaaaag
     1501 agcagtataa atcagaacag atggccttgt tttaaggggg cttacttttg aaaaacaaga
     1561 aaactgaaaa gttatatcgc taattatctg cttgttaaaa atgcaagaaa attattttgt
     1621 atttttatcg gacattagtg aaatgtatat atcaatttct tacaaaattg gaaatttcaa
     1681 agtgtcgaag tcaggaaaaa gcatgttgaa ctacattgta tttcaaaata tcaaaaacca
     1741 gctgcagcga caaaggatta ctgagacatg agacattgta tttattgtac atccgcatta
     1801 atgccagaaa aagattcaga gactgtctgg aatttatttc tgtgaccaaa gcaacacgac
     1861 cttcaatagt ccgaaatgca atggaggaca tggaagagtc ggttgcagag atctctgccc
     1921 aactattcca tgttcttcgt ggattttata ctgcagggca gtactgcgaa ctatggaatg
     1981 ttggaccacc gtcttcgatt actggcatta taaaggatga tcattttgtt tttacatgct
     2041 gacacaaaaa aatccccgtc aattttcacc aacggggatt caggttgtat tggtttattt
     2101 gcaatctcta aacgaggttt gcaaagccca taaatgcaag ggataaaata ccagccacca
     2161 caagcgcaat gggaactcca cgcatgcctt tcggaacgcc catcaggtca agttgttcgc
     2221 ggataccggc aaacaaaacg agcgcaagcg tgaaaccaac tgcagtggaa acagcaaata
     2281 ccaatgcttc gccaagattg aattctttgg tgataaccat aatggcaacc ccaaggacag
     2341 cgcagttggt tgtaataagc ggcaggaaaa taccaagcgc ctgataaagc ggcggcgaca
     2401 ttttcttcag aatgatttca accatctgca ccagtgaagc aatcacaaga atgaaaacaa
     2461 tggtttgcag atattgcaaa gagaatactt caagaacata aacctgaatc aggtatgtta
     2521 ccaatgtagc aagggtcatc acaaaaacaa cagctccacc cataccaacg gcagtcgaaa
     2581 tctttttcga cacccccaga aacggacaaa ctccgaggaa ctgagcaaaa ataatgttgg
     2641 agacaaatat ggcggatata acaattacaa tgtattccat aacgctgtgg ttttatttct
     2701 ttttaaaact gttgacaagt gcaatcagat atcccagggc aatgaaagcg ccgggagcaa
     2761 gcacaaaaac aaggattccg tccatgtcgg cggggatgaa cttataatta aaaatggaac
     2821 cgccccccag gatttcgcgg atagacccca gtaaaccgag tgcaaaagta aatccgagtc
     2881 ccatactgag accatcaata aatgagctga aaacgttgtt tttagaggca aaagcttcag
     2941 cacgtccaag cacaatgcag tttacaacaa tcaaaggaat gaagaggccc aattgagcgt
     3001 gaagatcggg aatgtatgct ttcatactca gatcgaccac ggtcacgaaa gaagcaatca
     3061 caacaatgaa agaaggaatg cgcaccgaag acgggatgaa atttttaatc agtgagataa
     3121 tgacattgga catgatcagc acgaaagtgg tggccagtcc cataccaatt ccattgatgg
     3181 cagatgtcgt tacgcccagt gtgggacaca ttcccagcag aagaaccaga acaggatttt
     3241 ctttgataaa tcccttggta aaattagtcc attgattcat tcctgacctc ctttccaaat
     3301 tgactgatga gcacgactga cagcatcgca gaaagcccgg ctggtaattg ttgaagcagt
     3361 aatggcatca acgtcgcctc catccttttt aacggcaagc ttataacttg aaggattttt
     3421 ctgcataaac tgatttttaa atttatcttc catcattttt gttccgagtc cgggtgtttc
     3481 ggcatgtgtc agaactttgg tattattgat ggttccatca ggtaaaaaac cgaccatcat
     3541 ggtgattaaa cccgagaatc cgtttttggt aaaagactcg acagcaatac ctgtggtatc
     3601 tgttcctttc acaccataat aaacggtaag actatcatcg gaatcggcac atttcacttg
     3661 cccttgtacc aatgtttcaa attcaggaat aacctcttta atggctgttt cgattttctt
     3721 tttctccgat aactcaatag gaccttttgt cagtgaataa acaccggcca gagcaaaagc
     3781 cgccactgct gaaataataa acagcgtaga ggtcatgtta aacaatgaat tctttttctt
     3841 agccattttt cactacctcc ccgaatcttt tgggtttgat tttatttaaa agcggcacaa
     3901 aggcgttcat gatgagaata gcaaaggaaa ctccttcggg ataagccccg aaaacgcgaa
     3961 tcaaaatggt caggaatccg cacataaaac caaatatcaa cattccaagc ggactcatgg
     4021 gtgatgttac catatccgtt gccataaaga atacgccgag catcatacca cctgtaatga
     4081 gatgaaacaa aggatcggca taataggaag gatcatacaa ccacaaagct cctgaaaaca
     4141 atacggctcc aagaatataa gaaacgggga tatgccatga aatgacccgg gtaatcagaa
     4201 gaacagcagc acctatcaat aaaagcaagg cagaaacttc gccaactgag ccgcccatat
     4261 tgccaaggag catattcatg taatcaggca tttcgggttt ggccatggcc gctgtaaaac
     4321 catcactttc tttcaataat gcaagtggtg taggtccagt cactacatca gtcagctgtg
     4381 acgctgattc aatgggtttg ggccacgatg tcatctgaac aggaaaagaa atgagcaaaa
     4441 atacacggcc aacaagagcc ggattgaatg gattctttcc caaaccacca aacgacattt
     4501 taccaatccc gatggccacc aaagcaccaa caatcaatat ccagacaggc agattacttg
     4561 gcacattgaa acccagcaaa atgccggtaa caacagcgga gccatcacca atggtggact
     4621 tatctttaag caaaaagcgc tgaatcagat actcaaaaag cacgcaggcc agcactgaaa
     4681 ctaaaaatac tttcacggca cccagcccga acaaatagaa tgatcccaga agtgcaggca
     4741 gcatggctaa aatcaccagc cgcatgactt ttccggcatc gcctctggca tgtaagtgcg
     4801 gtgatcctga aatggtcagc atattcatat tgtgttaatc cttttaattg tttctttttc
     4861 tgataatttg tccaaccttg tttttaccca gacggatgct gtcaagtaat ggtctgcctg
     4921 atggacaggt aaactgacat gaaccacatt ccatgcaatc catcacacgg ttgttttctg
     4981 caatttccca attctgattc tctccggcaa gacccagcag atagggctcc agtcccatcg
     5041 gacaaacagt ggtgcagcgg ccgcatcgaa tacaattgta ttcttttaca cgttttgcct
     5101 ctttttcatc cattagcaga attccgctcg tacccttggt tactgatatg tcggtcgaag
     5161 caaaagcttt tcccatcata gggccaccat tgatcatttt gccggtgcct tcgggaatgc
     5221 caccgcattt gtcaatcagc actgaaacgg gggttcctat gcgaaccatg aaattggatg
     5281 gtttcgagac ctttttcccg gtaaccgtta caacgcgctc aatcaaaggc ttgtttttct
     5341 gaacggcttc atatacagcg aatgtagttc caacattctg aacaacacaa ccaacttcaa
     5401 tcggtaattt gccactcggt acttcgcgat tgatggccgc tttgatcagc tgtttctcag
     5461 ctccctgcgg atatttcacc ttgagaccct gaaccgaaat acccggatat gatgctgaca
     5521 gttttgtcaa atgagcaatg gcatcaggtt tattattctc tattccgata atggctttcg
     5581 ttacttcgag tgctttcatc agaatctgaa caccaatcag aatctcttcg cctttttcaa
     5641 gcatcagacg atggtcggcg gtgagatatg gttcacattc aacggcatta atgataaggt
     5701 attctgcttt ttttccctga ggaaccatga gttttacatg ggttggaaat gcagcgccgc
     5761 caagaccgac aatgccagag ttttttatga tatcaatgat ttcagctttc gaagctttga
     5821 tttccttcac cagatcttta gaagtgtcaa tgccatcatt ccactcatca ccatcttttt
     5881 taataataat gaccggtttg cggtatcccg aagcatcggg ccatgaatcg accttttcaa
     5941 cagtacctga gacaggagaa tgaattgaag ctgagatgaa agcttcgcct tttgcaataa
     6001 gctgtccggt tttcactttg tcgcctttat tcacaaccgg aacagatgga gctccaaggt
     6061 tctgattcag catgacatat acagtgtcgg gagcttcgag tgtttcaatg gcgctgtgct
     6121 ccgaaagttt gcattcttcg gggtgaacac cgcccatttt gaaagtcttc aacatgtttg
     6181 gcctcctgaa atattattgg gttatttgat cttcgcctgc cggagcactt tcaatctttc
     6241 gtgccggaaa attgatttcc agaatggcac cggttggaca ttcagcaaca cacttgcgac
     6301 aaagtttaca cttctcgaaa ttgatatacg ccagattatt ttcgagcgta atggcatcgt
     6361 gcggacaagc ttttacgcat ttgccacaac cgatgcatgc agtaacgcag ttctttttag
     6421 ctacagcacc tttttctgta ttcacgcagg aaacaaaaat acgcttgctt ttcggccctt
     6481 tgttgcggag ttcaatgatt gatcgagggc aagctttaac acaggcaccg caggatgtac
     6541 aattatcttc gataacaacc ggcaaccctg ttgttttatc catatacatg gcatcgaatg
     6601 cacaactggc cacacaatcg cccagtccaa gacatccatt gggacaaccg ccggggccgg
     6661 catacaaact atgtgcataa gcgcaagaac tgatgccggc ataatctgat ctggccggag
     6721 agttggcaaa actgccctgg caacggacaa ctgcaacctg aggggctttt tcagcagcag
     6781 tcaatcccat cacagctgca acttcagcac tggtggcatt tcctccaacc ggacaaaaca
     6841 acccgtcgag actttcgttt ttcacaacgg cttcggcaaa attgcggcaa cccgcaaaac
     6901 cacatccacc gcaatttgca cctggcagct tttcgacaac cagatcgatg cggggatctt
     6961 caacaaccat aaatttcttt tcgactccga aaatgatagc ggcaaaaacc aatgcaacac
     7021 tacccaaaac aattactgca agtaaaatcg gatccacttt gtttcgtttt atttgggtgc
     7081 aaatgtataa aggataatta caatgatgcg tatttgcaaa agaattggaa tacgcttgca
     7141 aatcattaac attcaagtaa ttgcaaatta aataacagaa aaaaagacaa cagaatatgt
     7201 tcgttaaaat ctgttaatca ttcatttgca tatttaattt ataatctttc taaattaaac
     7261 atcaaaaact aatgttttta tataattctt aaaccagccc tcggccgctt ttttttattt
     7321 taccttccgc tttcaaatcg ctgatgattt tgtcaagcaa tccattgaca aaactgttgc
     7381 tcttgagtgt tccaaattcc ttcgagagct caatatattc attcatggac gccctcactg
     7441 gaatattgtc aaaatgtatt atttcggtca gagccagctt aataacgagc atatcaatga
     7501 caatgattcg ctcaatatcc cagtttttga gatgcttctc aatcaaatcg tcataagcgc
     7561 tgaggttgag tatggttttg tcgaaaagtt taatcataaa tgtccggtcg tcgtcatcgc
     7621 ctatgccttc tgatggcttc aacaaagcag ggaaaacggt agttgactct ttctccgggt
     7681 catagctttt aagccaggca tgaaccatca atccggtcaa cacggaatcg tcgccccaat
     7741 gaatgttttt ttcttcgtaa cggtcacgca gattgctgtc aaaagcaata aacttcttga
     7801 ataacttcac aataaactgt ctgtgcgaag cataatcgtg ctctcccttg tttgtgtaat
     7861 tctgatattc gctccaatcc agaacttctt tgtatatttt tcgaaaaata tcgttcatat
     7921 cggcccagtt cacacgcagg ccattgatgg cattctgcac atgggcatta tcacgcagat
     7981 gcatcagaag gggattctga acaaaagtca ggtttggatt gatatcctct ttggttggaa
     8041 cgtgtttatg gcgattctcc tcaatttttc tttcagctat atcgcgtaat tcgagaagtg
     8101 cagaaatctg aataaaaatt aaatcgcgca tatcatcaac tgaatcaact aaggccctgc
     8161 gccctttttc aagatctgta ttgccaccaa ctacatagga ataaacagct tgcagggttt
     8221 tgctccggat aatgtgacgc gataacatag agaacgtttt ggttttgcgg ctgcaaagat
     8281 acacaatagt ggtgagtagt gagtagtgag tggtgagttt ttttgaataa tgaacaggac
     8341 atgcatctta ctcactgctc gctgcatccc gctcactgat caccgctcac tactcaccgc
     8401 tcactactca ctgcttctaa acggctggct caggaatatc ttcctcaatt ctcaggttct
     8461 caataatgaa actctggcga tctggtgtgt ttttgcccat gtaaaacttc agaatttcgg
     8521 caagaccggt ttccttttta agtaatacat tttccaatcg cattttaggc cctatcagct
     8581 gacgaaactc atcggggctg atttcaccaa gacctttgaa tcgtgtgatt tccggcttcg
     8641 ggccaagttc ctgaatggca ctaacccgct cctgatcgct ataacaataa atggtttttt
     8701 gtttgttgcg cacacgaaat aacggggtct gcagaatata aaggtgacct gaacgaacca
     8761 gatcgggata aaactgaaga aaaaaagtaa gcagcaacaa tcggatatgc atgccgtcaa
     8821 catcggcatc ggtggctacc accacattgt tatacctgat attttctatg ccttcttcaa
     8881 ggttgatggc ggcctgcaga agattgaact cttcgttttc atacacaatg cgcttgctca
     8941 gaccgtaggt gttaagcggt ttccccttca aactgaaaac ggcctgcgtg tatggatcgc
     9001 gcgctttggt gatggagccg ctggccgagt cgccctccgt gatgaacaaa gtggtctgca
     9061 tgcagcgttc atccttgtct tcgttgaaat gcaacttgca atcgcgcagt ttcttattgt
     9121 gaagacttac tttcttgaaa ctttccttgg caattttctt tatgtttgcc agctccttgc
     9181 gctctttttc gttggccaga attttttgga gcaatgaatc ggatgtctca ctatgaatat
     9241 gaagatagtt gtccaaattc cggcgaataa tatctccaac gtaatttctg atggtttgtc
     9301 cgttgggctc catatactga gatccaagtt ttgtcttggt ctgcgactcg aaaacgggct
     9361 cgtgtacacg aatggaaata gcagcaacaa tggatccgcg gatatcactg gcttcaaaat
     9421 cctttttata aaacttgcgg acggtatcga ccaacgcttc cttgaatgcc gcaacatgag
     9481 ttccaccctg agttgtgtgc tgtccgttca cgaaggaata aatctcttcg ccgtaatttt
     9541 tatgactatg ggtgaaagca aattccagat cgccctcttc catatgaatg accgggtaaa
     9601 tggtttcgcc gtcaatattg cgttccagca aatcgagcaa tccgtttttt gattcgtatt
     9661 tttcgccgtt gaaattgata cagagaccct tgttcagata tgcataattc cacagcagcc
     9721 gctcgatatg ctcattgaaa tattcgtatt cgccgaaaat ggtttcatcg ggggtccacg
     9781 aaacgcgggt accacgctca ttggcgccaa cagtatcctt ggcttgcttg ataagattgc
     9841 cgccgctgaa atcggctgtt accttcttgc cctcgcggaa agattcaatg cggaaggccg
     9901 atgccagcgc atttactgct ttggttccga caccgttcag accgactgac tttttaaaaa
     9961 cctcactgtc gtatttggct ccggtgttga tttttgaaac aacatccaca acgctgccca
    10021 agggaatgcc gcggccgtaa tcgcgcacgg tgacttcgtt tcccttgatg gaaacatcaa
    10081 ccttctttcc atagcccatg atgaattcat caatggcgtt gtcgagaatt tccttaatca
    10141 ggacataaat tccatcgtcg tgcgaagctc cgtttccaag cttgccgata tacatacccg
    10201 ggcggagacg aatgtgctcg ttccacgcca gtgatttaat actttcttcg ttatactgct
    10261 ctgccataat gcgttctgct caattccttg caaagttaat acaaaccgga atatgtttca
    10321 aaaaagattt ctgagggggt taaaaaataa aacaggaact atacgccctg agccgcccga
    10381 aatgcggcca cagatcacgc cggtgcatat tgtcccatag cagtgcactg ccacagatgc
    10441 cggtgaagga tccgatccag aggtcgcgga tccaggaggc gtcgttatcg cacatgtaat
    10501 agccctggtt tgtcccggat ccgtattcac catgaataaa cggtttatcg caatatgtcc
    10561 agctatggga actgatcata gttttatcat caaacttgtt cccagataaa atggccagcc
    10621 atcatccaat acagaaacgt aatactccat atgagaaatt gacccatact tacgcgggga
    10681 caagcgaata caaggagcta ttttgggact aattccaaag gaacagtttt tcgacaagtt
    10741 ccagtaaata gccaacccta atttgttttg taaagcacga tctgtataat caatgaaata
    10801 tctattgttt gggtttgttt cggttgcatt aatgatatga ttatacaata attcatcaac
    10861 aatgctcaat gaaaacactt ttgataatct gaaatcaatt gaatagccaa caccaaagtt
    10921 caaataactt gcagtaaata tttcattcac gtagtgccaa ttaatactgt tttttttgaa
    10981 agccagacaa ataaataaat tatcttcaaa tttcatgatc atgctttttt tgaccctgat
    11041 tttagctccg gtttcaacag aaaaatttga attctgattt aatgaaaagc catctctact
    11101 acttggattc atctgaaaaa acaattctgc aacgaattcc ttctttttta cagaatctgt
    11161 ttgggcattg cctgataaaa ccggaattaa tgcagagagt aaaaaaagcc atttcatgtt
    11221 tttacttttt aattattact ggaattattt gtagatggcc atccaacaaa aatcgaatga
    11281 aatatattcc atcaggtgca ttacaacata aagtttgacc ttgaatttca aaggggatat
    11341 ccttcccatt ctcatctgta agctccaatg cagcattttg tggatcaaaa ccaagcaaaa
    11401 aacaaccatt gccaggatta ggataaatta agggatcggt ccattgcgtg taaaaattaa
    11461 aagtgcggtt agccaataca cctactgcat ttaatttatc gggtgagcgc agataaaata
    11521 aatcgacaat accgggcttg cgctgccagt tttcgtagtg catttcatca aaatcatgat
    11581 aaggaatcca ccctttctga tcaccgttgc tttcatcaaa atcatatcct gccacaaaag
    11641 ccctgagccg tccgaaatgc ggccacaatt cacgtttgtg catattatcc cacataagcg
    11701 cactgccgca aatgccggtg aaggagccaa tccagaggtc gcggatccag gaggcgtcgt
    11761 tgtcgcaatt cattttcatt cacgaacaaa aatatttgta tagcttgaat atatgccatc
    11821 attatcaaaa accggaaaat aaaacggaga actatactga tatgtaatca ctgtatcacc
    11881 aaagatcttt ccttccaagc gtgtatcagg attaattaca atgctaaaat aaaatatctc
    11941 agctgtgtca accggattcc aagtggcaaa ttttgccttc tctctatgga ttacatcatc
    12001 tccatcctta agaagatatt ttccgttttc tcgaaactcc atagaaaaag atttgttgat
    12061 atcagaagga aatatctgat gtgaaattac acctccacca ccaggatcag gatatttccc
    12121 aattgagtgt acccattgcc agttaccaac aaaaactgtc ttatctccca ttaaatcata
    12181 gttttcacag ggagccaaaa ccattgctga taataggaaa aaacatgata atattttgga
    12241 gaatttgtcc ttattcacga acaaaataac ttttataagt ggaatatgaa tacccatgat
    12301 ctgtttcaaa aggcgggaaa tagttactgg tcacaataga atcctcaagg attttcccat
    12361 aaaattgctg gtctttatca atataaaacc aaaaataaaa gcaggttcca ctatcaatag
    12421 actgccaatc aaggaatttt gacttgtcct tataccaaac atcatcgttt tcaaataatt
    12481 tatatttccc attttctctg aattccattg agtaatttgt gttttcactt tccggagtca
    12541 atgtccgata ataaatgagg ccattatcat gtacttcttc aattgaataa acccatttcc
    12601 aatttccaat gaatatggct ttatcaccaa tcagatcata ttcactacag gaatacaatc
    12661 ctaatatcaa tccaataaaa atgatggaat ttttaattct tgatgaattt ttcatggtag
    12721 gaatcctcct cgttttgaac aacaataata taaataccag gtactatctg atcaagcggg
    12781 atttgcaggt tttgtccggt catggtcaat agaagtatca acctcccttc agtagaaaat
    12841 atctgaacaa ttgattctgc ttgtatgccg ctataagtaa tgttaagaat atcatttgct
    12901 ggatttggaa acacctttat tcctgacttc acaacagaat tatctacagt ggtagcattt
    12961 cccatattca tcaaactatt gctttcatcc gaatatctgt taagagtatc caagcattcc
    13021 agcatcgaac gatctttcca cttataaatc ttgaatagta caattgggca ttttgcattt
    13081 ccagttagct gtggatattc taaaaaaagt tctccatcaa catcacttct gactttttca
    13141 aatgtttgaa taaactgtcc agtcataaca ttgtaaaatt caatattata gcgtacaaac
    13201 gagcccattt tggggatcat aagtaattcc caataattag cggagtttcc gactggacca
    13261 atgtttaagg cgctgttgta aggcaccatg gggatgtcgg cttggcactc tcccccttca
    13321 gcttgagtat aataattata cgttctgttt gcaataactc cgattgcctg tttatgatta
    13381 tcttcccaat tgcgcagaca catcatttct gcaagattat cacctcttaa atcattttca
    13441 tttgtccatc ctttatgctg gctgtcaccg ctattgtcaa aatcaattcc ttggacgaaa
    13501 ctgcgcaaac gcccgaaatg tggccacagc tcacgtttat gctggttatc ccacagcaat
    13561 gcactgccac agatgccggt gaacgaacct atccacaggt cgcggatcca ggaggcgtcg
    13621 ttgtcgcaca tgtaatagcc ctggtttgtc ccggatccgt attcaccatg aataaacggt
    13681 ttatcgcaat atgtccagcc aaaggaactg gcattgctat tatcatgcag atcattcaca
    13741 atatctacag tacttttata tatatccgga aaatctctgt agttctgttg agtaatgata
    13801 tccaatgaat tcagataata tgtggtgtcg tctgaaatgt tgggcccgtc agttaaataa
    13861 ttcacactcg tcagatgctg gttatggccc atgtatgcag tcatcgaatt aagcatgctg
    13921 ctttgccact cattcacgtt ttgtctgaaa agtacttcct gattataggg ggaataacca
    13981 gatcccggca caacgttaca tgtactgtca tccggattat aataaacctc tttagttccg
    14041 catgtgccgt ttatttcatt gattgtttca aatgcggaaa tatttgtaga atatccccat
    14101 cgggcaatca tatagcgcaa tcgtttttgg taataatctt tcgcataata atcagagaaa
    14161 aataattttg ggtcagtcag tcccaaatct gaattataac aataaccatg atccgagttg
    14221 ttgtcacatt cttccggcaa gccagcttcg ccataagcag accagtccca cagcagatgg
    14281 tcaggtttaa gaaaataata ttgaattgct gtgttgaaat gaataaacag tccttcctgt
    14341 tccgatttct caacaatatt gtcaacttcc catccgatat gcatccggtt gtagtaattg
    14401 caaatctgtt caa
//
LOCUS       DAOPKE010000068        14333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4431_length_14333_cov_13.093781, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000068 DAOPKE010000000
VERSION     DAOPKE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_14333_cov_13.093781"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PKY63_09090"
     CDS             complement(<1..1032)
                     /locus_tag="PKY63_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HPF00807.1"
                     /translation="MKIEQKHIDTIRSQFAEMQNKEDLALLLSAAKNILYGKVCKPIS
                     VKALNYYANPEYCKQRYTTFTIKKKSGGVRTINAPVDGLKSKLRALNFALQCLYEPHK
                     AATGFVREKSIVDNAKCHVGHNYVFNIDLKDFFHSFDRNRVKMGFMSEPFNLNGEKEP
                     LAFLLASLCTHSFEIDGTQQIVLPQGSPTSPTITNILCGKLDHRLSGLAKRFGAKYSR
                     YADDITFSSDHNIYNKPDFRNELTRIIEENQNLKINPDKTRMQKTGYRQETTGLIVNE
                     KVNVTRRYVKQIRMYLYYWEKYGYEKAEQIFKKDYSTDKGLNKKKSSRLLNVIDGKLE
                     FLKMVKGSED"
     gene            1384..2400
                     /locus_tag="PKY63_09095"
     CDS             1384..2400
                     /locus_tag="PKY63_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF00808.1"
                     /translation="MPDTKHALIRYKILDNCFRNPGRKYFLEDLMREVDIVLQEINPE
                     KKGISRRQLLYDIEFMESTEGWAVELNKVREGHKVYYRYADMSFSINNMPLNEVEINQ
                     LKDAVSILSQFKGMPQFEWVYELIPKLQQGMDNNLNQPSVISFDNNEYLKGVEYVGIL
                     YSAIMFKHVLKIGYHPFDAPSPIEIEFHPYYLKQYNNRWFVLGLNMQQNKIWTLALDR
                     VVSTEELTRAKYLKTDENWDEFFDDIVGVTIPENGKVEEVILKFFGLSGNYIETKPIH
                     GSQKAKWLSPDCLEVRLNLMINYEFEQLVLSYGENVKVSAPEHLVEKMKMRIRGMKEL
                     YERT"
     gene            complement(2476..3096)
                     /locus_tag="PKY63_09100"
     CDS             complement(2476..3096)
                     /locus_tag="PKY63_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00809.1"
                     /translation="MEVLIVAKTHMKNGVCVGGYDITNKRNVRLLGPNAENQPFDTDL
                     NVGQVWDIAYSNRETIIPPHIEDVLIASRKFLRTESNMYSFLIKSAPIWRGDPTCIFE
                     GKVDFPRGKSCFIPNPQNLPSQSVGFWISDDDLFLTIFSDQRHYYYFNEYGDVYTIPF
                     VGFQPVQKVIKKGSIIRVSLARWWSPPGSNSDKKCYCQMSGWYDVG"
     gene            complement(3098..3577)
                     /locus_tag="PKY63_09105"
     CDS             complement(3098..3577)
                     /locus_tag="PKY63_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPF00810.1"
                     /translation="MKIYTIGVYNSTEQEFFEKLVASQIDTFCDIRQRRGVRGSKYAF
                     ANSLRLQKKLAELKIKYLYEPGLAPTSEIRSLQKEVDLKLGEQKKDRQHLGSVFVQEF
                     NNRILSNFNFTRFNSALIAEGADKVVFFCVEELPGACHRSLVANKIKSEFMYDVKHL"
     gene            complement(3584..4135)
                     /locus_tag="PKY63_09110"
     CDS             complement(3584..4135)
                     /locus_tag="PKY63_09110"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPF00811.1"
                     /translation="MEKNPLYTIGHGHRKQEEFLELLRKFEILYLIDVRSQPYSKFNP
                     GFNQSEIKIFLENNSVKYVYMGDTLGGMPKDISCYTIRGNVDYEILKTKEYFLNGIER
                     LKTAYNQNLKIAIMCSESKPTECHRTKLIGRVLSEQDISVVHIDEKGNLKDQITVINE
                     LNKGCAEIDLFGEKKNTASRKTH"
     gene            complement(4236..4508)
                     /locus_tag="PKY63_09115"
     CDS             complement(4236..4508)
                     /locus_tag="PKY63_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00812.1"
                     /translation="MFEILNFTGKYNNNQKIIHHSQRCWIFKLSYKQIDGQLDYNRIN
                     IEILNSSSREKVSLTWAELIKAAMEEFKIENNIGPDLKGRKSQEEE"
     gene            4507..4968
                     /locus_tag="PKY63_09120"
     CDS             4507..4968
                     /locus_tag="PKY63_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00813.1"
                     /translation="MKKIFEYTADFDNYDSEEPYNEVTYRLLIKHDNSQNLISQKFSF
                     SFLDLQGHSIDDFVVNSWKIRRIYSEDYFGRAIVCVNVVDPVGSILQPLKSGGFTLSL
                     DERQNINDIENKLYMLLKQVRRFSCFMNLRSRLDYVDIVSSGEDAVPYHKL"
     gene            5182..6222
                     /locus_tag="PKY63_09125"
     CDS             5182..6222
                     /locus_tag="PKY63_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00814.1"
                     /translation="MKIIYPLPNPADKEVREKLLKVLEILGFPECSGLDLTLDQYYNS
                     FHFAKDVMLDFVERHSRDAEKLKEGFGIVSELLEKYPNHGIVLKFAAHLNCLCGDREL
                     AYEQYMRLIYARRSPDAIMPMALFDFPEKSKELNYEALMAKIASSRFTYADEYFKAFV
                     AAEPNHPTIYLQCAITIIQRFNKGKQMEDAMEMLENHLRILPDDERAWQYKIVALKKQ
                     QEYDNALQTAQQAIMKFGSSSVFHNERGEIFLILQNIDSAREDLQTALELNPVYVPAI
                     VNRGILYISEQRFDDAMFEFNRAIEMNKEYATAWECRGNLHRLLGNEEEAQADLQQYH
                     IMKLGEMIKKIL"
     gene            6286..7188
                     /locus_tag="PKY63_09130"
     CDS             6286..7188
                     /locus_tag="PKY63_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF00815.1"
                     /translation="MSYQGTLHRWLLLLQKTGGSSYPSFEEIKSFLHESDFEISTRTL
                     QRDIEQIRTEFGIEVLYNRTEGGYYIDYELSPDFESTMRFFEIISTAGILSDSLRDSK
                     QALKHIIFDSQGRLRGTEHLKTILQAIRGKKMVGFKHENFGTGKSYQFKVAPYLLKEY
                     QNRWYLIGIPAGMNDFRTFGIDRMDDLKMLAESFRYDKKLNPLDDFEHIVGLTYSDRK
                     VETVVISVDPFQGKYLKALPLHASQKILIDNAKELRISLRIKPNFEFKQQLLMLGDRA
                     QVLEPAWLVKDIKKTIDAMAKKYK"
     gene            7245..8075
                     /locus_tag="PKY63_09135"
     CDS             7245..8075
                     /locus_tag="PKY63_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00816.1"
                     /translation="MINKESIKIRENMLSETYDRCFTSMVKNFCDNSDNANTKYYAFY
                     PSFGVKKNQPLDFLVYGQAVNGWHDDSAFLVSDKKSTIGVCKKSKEYSNSYFPYDNHG
                     PLDWVNVQWCKSEYDKFLRQSKAEAEFKRYYIDKYRAYSSFFWNVVYKTVSDKMNYSR
                     ETRSWADSVVWSNLYKISPHKGNPNGKERGLQIKYAIQLVKMELDELKPNYCLVLTNL
                     KWWDPFAKELRLEICSQKETKRIQQVAYYGNTKIIVTKRPFLGDSEAYSREILNSITK
                     "
     gene            8072..9061
                     /locus_tag="PKY63_09140"
     CDS             8072..9061
                     /locus_tag="PKY63_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00817.1"
                     /translation="MITNLKPHLTIDAQTVECPVAGCTHKVERQKNSFKKEPRFQCPE
                     HRIFISPSTFEYEKEEENLLWTDDSDMELFSAIKTVKRESRIARENSEDAVTWNVFRY
                     LERQNLLPALLNESFASQTTTAELMLWSFNQREYQQQKENFCGWSLLNEARLTFGETI
                     AHGSEPDIIINTDHTLVFVEAKVTSGNDTSGSGETLARHLAAPSGYTTGANRWYNTVF
                     NSPYEKVVRDQKYELLRFWLLGTWMAKQMNKRFLLANLVPADKEQSILWEFGTHIKEP
                     DAGIFTRITWEDIYNAIVKTNLSNSDTDKMLHYFRNKTLGYDSSKNLIKAFKI"
     gene            9066..9758
                     /locus_tag="PKY63_09145"
     CDS             9066..9758
                     /locus_tag="PKY63_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00818.1"
                     /translation="MKLISYYNEDLEKILTAVFGIVGIAAIFINLDIKGYGNENWLDA
                     IKDIAGLIVVLAVFIAAIRIRQKSETHYEMARNALQHLQAKHPQILMGPRYNREGYDP
                     EKGKGLEYLFVTNENRKSTLRAKLIPIQPLEEGCLYIYIQTATLADALNYGRGNVTDE
                     DVSKVKDAVKEAVNTILNAGYKGKFETLPAVKDSVIAIDFNESDMRKKQFAKAVADCT
                     EAATLKLLEFRK"
     gene            10020..12113
                     /locus_tag="PKY63_09150"
     CDS             10020..12113
                     /locus_tag="PKY63_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF00819.1"
                     /translation="MMNKKELSEKRKMLQNARVELKKEFFGIDKIIDEVCRVVESWVL
                     FPEMQERPLVINLWGMTGVGKTALIKRLSELIGKAEMFFRFDMGDNSSNWTIRETLTD
                     VLEKENSRPMILVFDEFQNASSTGKDERESRATRLVWDILDNGRFNSNTIRFSNFEKL
                     QRLIYASQFMLSKGVTVKNGFVTSGMSIFRNYSVLKSSEFYRHAEKSYKHERGNKFPF
                     VADSYLDDLLDFLWPKFKTEFEIREYLDTLNGTQTVDFLTEIFHKSITPKTFDCSKSV
                     IFVIGNLDDAYSMSGNYNPDMSADDFHKESLKITRSDIKMVLSWSFRHEQIARLGNIH
                     LVYPSLSSNAYKRIIEESLKKISRNYLNKHDMDFVFSKCIHEMIFAEGVTPAQGVRPI
                     QSTINQLIVSNIGQVINQMSKLRKKPDKAVFDVCNETVVVNFMKGKVQCGSMKYDVDL
                     ENARLRKIKHDDNQAIVAVHEAGHAVIDIVLLKVIPDRILSRTADASVNGFVDVSKKP
                     EFHSVSIIQNEIVSLFGGLAAEEIIFGHEHVTAGSGSDISQATRRATAMIRNRGMGSF
                     PGMVNLPQPILNEGYLDLDGALNNEVKSLLQESYSAALKIITENQSLLLELASILNAN
                     ACIDKEQIRQLLCTRFPHLSAQATGNSSSGFSYRHHLLRKCAAASTQNPSCSLNQMPA
                     MVMNMEKTEKEIQPE"
     gene            12145..12864
                     /locus_tag="PKY63_09155"
     CDS             12145..12864
                     /locus_tag="PKY63_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00820.1"
                     /translation="MNTFEEKYLYKIKDLFEKYKGKMRDPELNEILDKGFVSSKPNLN
                     AKILLCGINPSDNGKEEKNFHFHDLASAPYFRTLHDVVRECENKNSIDYLDLFCFRNT
                     NQYDIWQFAKDSVGLRFVVDHLILTQEIIEDIQPKVILVFNKTAATFFGYHAAPDDAN
                     PTNQKIWMGYKLEDSSMTDNMYSAQMKYITGLHEGRMNTELKETSLRGSKIFFGSYMR
                     YLKNEKKEELRKLIASLSKFE"
     gene            complement(12933..13625)
                     /locus_tag="PKY63_09160"
     CDS             complement(12933..13625)
                     /locus_tag="PKY63_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPF00821.1"
                     /translation="MKKISVIAFDADDTLWVNEPYYQETEKMFCAMMHEYATPERVSE
                     VLLQTEKQNIGLYGFGAKGFTLSMIETAMHLSDGKAGGNIVSGIISLGKALLDKPVKL
                     LGDVLTVLSDLHSRYTMVIATKGDLLDQQRKLSKSGIEKMFHHIEIMSDKTEADYQKL
                     LMKLNARPEEFLMIGNSMKSDVLPVVAIGGTAIHIPYSVTWQHEAETEPVSHPKILTA
                     KRMGEILAMLEN"
     gene            complement(13627..14127)
                     /locus_tag="PKY63_09165"
     CDS             complement(13627..14127)
                     /locus_tag="PKY63_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00822.1"
                     /translation="MSKIIFEMVEKIYHFELKDYRPDGVNIETRTPFVLLIGEWEVDF
                     YEANKPFKANSVHGNFRFMKLLQNSFLMENNYRLGFDEIDGEIDLDAAIKMDFPASEY
                     QTVYALGSCFDEDEALFLISDETLPDGVALLKYTPDADNDDPESEINPAGVGENELSK
                     SIKKKV"
BASE COUNT         4330 a         2807 c         2879 g         4317 t
ORIGIN      
        1 atcctcagac cctttcacca ttttcaaaaa ctccagtttc ccatcaatta cattcaatag
       61 acgggacgat ttctttttgt taaggccttt atcagtgctg taatctttct tgaaaatttg
      121 ttcagctttt tcatagccgt acttttccca ataatagaga tacattcgta tttgcttgac
      181 atatcttctt gtcacgttca ctttttcatt tacaatcaaa ccggtggttt cttgccggta
      241 acccgttttt tgcattctcg ttttatcagg attgatcttc agattctgat tttcctcaat
      301 gattctcgtt aactcatttc tgaaatccgg tttattataa atgttgtgat cagatgaaaa
      361 ggtgatgtca tctgcataac gggaatattt tgctccaaat cttttggcaa gaccactcag
      421 tctatgatcc aactttccac aaagaatatt ggttattgtt ggggatgttg ggctgccctg
      481 aggcaatact atttgctgtg tgccgtcaat ttcaaaagag tgtgtacaaa gacttgcaag
      541 caagaaagcc agtggttctt tttcaccatt taaattaaat ggttcggaca taaaacccat
      601 tttcaccctg tttctgtcaa atgaatgaaa aaaatctttc aaatcaatat taaaaacata
      661 attgtggccg acatggcatt ttgcattatc gacaattgat ttttctctca caaaacctgt
      721 tgcagcttta tgcggttcat aaaggcactg taatgcgaag tttaatgcgc gtagtttaga
      781 ttttaatcca tcaacaggag cattgatggt tcttacgcct cctgactttt tcttgatagt
      841 aaatgttgta taccgctgtt tgcaatactc tggattggca taataattca gcgccttaac
      901 acttattggt ttgcacactt tcccgtaaag gatgtttttt gcagcactta agagcaacgc
      961 cagatcctct ttgttttgca tttcggcaaa ctgcgatctt attgtatcaa tatgtttttg
     1021 ttctattttc atttttcagc tattatgctc tccgaaaaca cctgcctgat actctttatg
     1081 tatgagttcg aaatgatctc aatcttacat agcttacata gctttatatc atgaattgca
     1141 ttcatcgcta taaatgctac gcaggctctt gacatgtcca atggacattc atcccagcca
     1201 aaaaggcggg aaaagatgcc gatcattgtt tcattggcaa accaactact cccggcatcc
     1261 ggaatcaata tcgtaggcag gtgtttcaaa gaactgcagc aaaactatat tgtaatagtg
     1321 caatcaattt gcattgttgc gaaattttaa tatatttgct tcaaatgtaa aaaattcttc
     1381 cgtatgccag acacaaaaca tgcattaatc cggtacaaaa ttctcgacaa ttgtttcaga
     1441 aaccccggcc gcaagtactt tcttgaggat ttgatgagag aagtcgatat tgtcttgcag
     1501 gaaattaacc cggaaaagaa aggaatcagc cgtcgccagt tattatatga tatcgaattt
     1561 atggagagca ctgagggctg ggcagttgaa ctgaacaagg tcagggaggg gcataaagtg
     1621 tattatcggt atgccgacat gtcattctca atcaataata tgcctttgaa cgaagtagaa
     1681 atcaaccagc ttaaagatgc agtttcaatt ctgagccaat tcaaaggaat gccgcagttt
     1741 gagtgggttt atgagctgat accaaagcta cagcaaggga tggacaacaa tttaaatcaa
     1801 ccctcagtga tttcttttga caacaatgag tacttaaagg gagtggaata tgttggcatt
     1861 ctttattctg cgataatgtt taaacatgtt ctcaaaatcg gataccatcc atttgatgcg
     1921 ccatcgccta ttgaaatcga atttcatcca tattatctca agcaatacaa caatcgctgg
     1981 tttgttttgg ggctgaatat gcagcagaat aaaatatgga ctcttgcttt ggatcgtgtt
     2041 gtttctactg aagagttgac aagagcaaaa tatttgaaaa cagacgagaa ctgggacgaa
     2101 ttctttgatg acattgttgg cgttacaatt ccggaaaacg ggaaagttga agaagtgata
     2161 ttgaagtttt tcgggctttc cggaaattac attgaaacca aacctattca tggttctcaa
     2221 aaggcaaagt ggttatcacc ggattgtctg gaagtcagat tgaacctgat gattaattat
     2281 gaatttgagc aattggtgct gtcgtacggg gaaaatgtga aagtaagtgc tccggaacat
     2341 ttggttgaaa aaatgaaaat gaggattcga ggaatgaaag agctctatga acgcacgtaa
     2401 tcaggaatta ttttaataat agtggtacgc tggatggaat gccgcttgat taaattcaat
     2461 tatttatttc aggtcttacc ccacatcgta ccaaccagac atttgacaat aacatttttt
     2521 gtcactattt gagcccggag ggctccacca tcttgccaaa gacactctaa tgattgaacc
     2581 ttttttaatt actttctgca caggctgaaa tccaacaaaa ggtatagtat aaacatctcc
     2641 atactcgttg aaataataat aatgtctctg gtcactgaaa atagttaaaa aaagatcatc
     2701 atcagatatc caaaatccaa cactttggga tgggagattt tgcggatttg gaataaaaca
     2761 actttttcca cgaggaaaat ccacctttcc ttcaaaaata catgtaggat ctcctctcca
     2821 aatgggtgcg gattttatta aaaaagagta catattactt tctgtacgca aaaattttct
     2881 ggaagcaata agaacatctt cgatatgtgg aggaattatt gtttctctat ttgagtatgc
     2941 aatatcccaa acctgaccga cattaagatc tgtatcaaat ggctggtttt cggcgttggg
     3001 ccctaataaa cgcacattcc gtttattagt aatatcatac ccaccaacac aaacgccatt
     3061 tttcatatgt gttttagcaa caattagtac ttccatgtta taaatgtttt acgtcataca
     3121 taaactcact tttaatcttg ttggcgacta atgatctgtg gcatgcacca ggcagttctt
     3181 caacacagaa aaacacaact ttatcagcac cttcagcaat taaagcggaa ttaaaccggg
     3241 taaaattaaa gttgctcagg atgcggttat taaattcctg cacgaaaaca cttcctaaat
     3301 gttgtcggtc ttttttttgc tcgcccaatt tcaaatcaac ttccttttgc aaacttcgaa
     3361 tttcagatgt aggcgctaaa ccaggctcat acaggtattt aatttttaat tctgccagtt
     3421 ttttttgtag tcggaggctg tttgcaaaag catattttga tcctcgaacg ccgcgccgct
     3481 gacgtatatc acaaaaagta tcaatttgtg aggccactaa tttttcaaaa aactcttgtt
     3541 ctgttgagtt gtaaacgcca atagtataga ttttcatgtt ttattaatgt gtttttcttg
     3601 aagctgtatt tttcttttca ccaaataaat caatttcagc gcaacctttg tttagctcgt
     3661 ttattacggt tatttggtct ttcaaattcc ccttttcgtc tatgtgaaca acagaaatgt
     3721 cctgttcgct aagcactcgc ccaattagtt ttgtccggtg gcattctgta ggcttgcttt
     3781 cactacacat aatggcaatt tttaaattct gattatatgc tgtttttagc cgctcaattc
     3841 cgtttagaaa gtactctttt gtcttcagta tttcataatc cacatttcct ctaattgtat
     3901 agcaggaaat gtcctttggc ataccaccga gagtgtctcc catatatacg tactttactg
     3961 aattattttc aagaaatatt tttatctccg attggttaaa gccggggtta aacttagaat
     4021 atggctgaga acgcacatca atcaaataca gaatttcgaa ttttctcaaa agttcaagaa
     4081 actcctcttg tttcctgtgc ccatggccaa tagtatatag tggattcttt tccatttaat
     4141 cggcaaggta ctaaaaatta tttatttggt ggatgtggtt tgggatgtat taatgaatgt
     4201 ttttagccct gcattttttc tgctcaattt ctctcttatt cttcctcttg gctctttctg
     4261 cctttcaagt ctggtccaat gttattttct atcttaaact cctccatggc tgcttttatc
     4321 aattctgccc aagtaagact gactttttct ctgctagacg aattaagtat ttcaatgttt
     4381 attctattat aatcaagctg tccatcaatt tgtttataac tcagtttaaa aatccaacac
     4441 ctttgggagt gatggattat tttctggtta ttattatatt tgccagtaaa atttaatatt
     4501 tcgaacatga agaaaatatt tgagtataca gccgactttg acaattatga ttcagaggag
     4561 ccatataatg aagttaccta ccgcttatta attaagcatg ataattcaca gaacttaatt
     4621 tcgcagaagt tttcattttc atttttagat ttgcaaggtc actcaattga tgattttgta
     4681 gttaactcat ggaaaattcg cagaatttat tccgaagact attttggcag ggccattgtt
     4741 tgtgtaaatg ttgttgatcc agttggaagc attttgcagc cccttaagtc aggtggtttt
     4801 actttaagtt tagatgagcg acaaaacatt aatgatatag aaaacaaatt atatatgctg
     4861 ctaaagcaag tcagaaggtt ttcctgcttt atgaatttac ggtccagatt ggattatgtt
     4921 gatatagtgt catcgggaga agatgcagtg ccttatcata aattgtaaaa gttgtaagct
     4981 tttttcagtg ctgtatcgcg gtttctgaat gtgcaagagc ctgtctttag cctgaatcgg
     5041 acagaacttg ctttttgaaa tttcatgaat ttcattcttc tcaagtaata gtttctgaac
     5101 atttatcctc aaataaaata ctggcgtcgg aaattgagca cttcgtttga aacaaacact
     5161 ttatccttac ctttatcccc aatgaaaatc atctacccac tcccaaaccc cgctgacaaa
     5221 gaagtgcgcg aaaaacttct gaaagtcctg gaaatccttg ggtttccgga gtgctcgggc
     5281 ttggatctta cgttggatca gtattacaat tcatttcatt ttgcaaagga tgttatgctt
     5341 gattttgtag agcggcattc acgcgatgcg gaaaagctga aggaaggctt tgggattgtt
     5401 agtgagctgc ttgaaaaata tccgaaccat ggcattgtgc tgaaatttgc cgcgcatctg
     5461 aactgcttgt gtggtgaccg cgaacttgca tatgaacagt atatgaggct gatttatgcg
     5521 cggcgttcac ctgacgcaat aatgccaatg gcgttgtttg attttccgga aaaaagcaag
     5581 gaactaaatt atgaagccct gatggctaaa attgcttcgt cgcgcttcac ttatgccgat
     5641 gaatatttca aagcatttgt tgccgctgaa ccaaatcacc cgacaatata tttgcaatgt
     5701 gccattacga ttattcagcg gtttaataag ggaaaacaga tggaagacgc aatggagatg
     5761 cttgaaaatc atttgcggat tttgccggat gatgaacgcg catggcagta taaaattgtt
     5821 gctttgaaaa agcagcagga gtacgacaat gctttgcaga ccgcacagca ggccataatg
     5881 aagttcggca gcagcagcgt gtttcacaat gaaagaggcg aaatatttct gatccttcag
     5941 aatattgaca gcgccagaga agatctgcaa actgcgctgg aacttaatcc tgtatatgtc
     6001 ccggccattg tcaaccgcgg aatattatac atcagtgaac aacgtttcga cgatgcaatg
     6061 tttgaattca accgtgcgat agaaatgaat aaagaatatg ctaccgcatg ggaatgccgt
     6121 ggcaacctgc atcggttgct gggtaacgaa gaagaagcgc aagctgattt gcagcaatat
     6181 catattatga aactggggga gatgattaaa aaaattcttt agtaatatca acttttcacg
     6241 agattgatcg cattttgtat atttacaaaa attatttatt tatccatgtc ataccagggt
     6301 accctacaca gatggctatt attgttacag aaaacgggcg gatcatcgta tccttccttt
     6361 gaagaaataa agtcgtttct gcacgagtct gattttgaaa tttcgacccg gacgctgcag
     6421 cgcgatattg aacagatacg cactgaattt ggcattgaag ttttgtataa ccgcactgag
     6481 ggcggatatt acatagatta tgagttgtcg cccgactttg aatctaccat gcggtttttc
     6541 gaaatcatca gtacggccgg cattttatcg gacagtctgc gtgacagcaa gcaggccctg
     6601 aagcatatta tttttgattc gcaggggcgg ttgcggggaa ctgaacacct gaaaaccatt
     6661 ctgcaggcca ttcgtggcaa aaagatggtt ggttttaaac atgagaattt tggaacagga
     6721 aaatcatatc agttcaaagt tgctccctat ctgctgaaag aatatcagaa ccgctggtat
     6781 ctgatcggca ttcccgcggg catgaatgat ttcaggacct ttggcattga ccgcatggac
     6841 gacctgaaaa tgctggcgga aagttttcgt tacgataaaa aacttaatcc acttgatgat
     6901 ttcgagcaca ttgttggact gacctacagc gacaggaagg tagaaacagt ggtgatttcg
     6961 gtcgatcctt ttcaggggaa atatctgaaa gcgctgccat tgcatgcttc gcaaaagata
     7021 ttgattgata acgccaaaga gctccgcatc agccttcgga taaagcccaa cttcgaattc
     7081 aaacagcaac tgctcatgtt gggcgaccgg gcccaggtgc tggaacccgc atggcttgtc
     7141 aaagacataa agaaaaccat tgatgccatg gcaaagaaat ataaatgatt tttaactacg
     7201 ccgtgttttg gcggagagag attattaatt tgcgaaaaac cattatgatt aataaagagt
     7261 caataaaaat tcgtgagaac atgttgtcag aaacgtatga ccgatgcttt acaagtatgg
     7321 ttaaaaactt ttgtgacaac tctgacaatg caaacaccaa gtactatgct ttttacccca
     7381 gtttcggggt taaaaaaaac caacctctgg attttttggt ttacggacaa gctgtgaatg
     7441 ggtggcatga tgacagtgca tttttagtta gtgacaaaaa atccacaata ggtgtttgca
     7501 aaaaatctaa ggaatattca aattcgtatt ttccatatga taatcatggt ccacttgatt
     7561 gggtcaatgt acagtggtgc aagtcagaat acgacaaatt tttgagacag tcaaaagcag
     7621 aagctgagtt taagcgatat tacattgata aatacagggc atatagttca ttcttttgga
     7681 atgttgttta taagactgtg tctgataaaa tgaattatag cagagaaaca agatcctggg
     7741 ctgattctgt tgtgtggtct aacttatata aaatttctcc tcataaagga aatcccaatg
     7801 gaaaagaacg gggtcttcaa ataaagtatg ccattcaatt agttaaaatg gagttagatg
     7861 aattaaagcc aaattattgt ttggttctta caaatcttaa atggtgggat ccgtttgcta
     7921 aagaactccg gttagaaatt tgtagccaaa aagaaacaaa aaggatacaa caagttgcat
     7981 actatggaaa tacaaaaatc attgtaacaa aaagaccttt tttaggagat agtgaagcat
     8041 attcaagaga aatacttaac tcaattacta aatgattaca aatttaaaac ctcacctcac
     8101 catcgacgct caaactgtcg aatgtccagt tgcaggctgt acgcacaaag tggagcgaca
     8161 gaaaaactca tttaaaaaag agccgcgttt tcaatgtccg gagcaccgga tatttatttc
     8221 tccttcgacc tttgagtatg aaaaagaaga agagaatctg ctctggacag atgattcgga
     8281 catggagctg ttttctgcca tcaagaccgt caaacgcgaa agccgcattg cgcgcgaaaa
     8341 cagcgaagac gctgtaacct ggaatgtgtt ccggtatctg gaacggcaga acctgttgcc
     8401 agccttgctg aatgagagtt tcgccagtca aaccaccacg gcggagttga tgctgtggtc
     8461 gtttaatcag cgcgaatatc agcagcagaa agaaaatttc tgtggctggt cgttgctgaa
     8521 cgaagcacgc ctgacgtttg gagaaaccat tgcgcatggc tcagagcccg acattatcat
     8581 caataccgat cacacgcttg tttttgttga agccaaagta acttcgggca acgatacatc
     8641 gggcagcggc gaaactctgg cgcggcatct tgcagcacca agcggttaca ccacaggcgc
     8701 caatcgttgg tataatacgg tgttcaactc accatacgaa aaagtggtgc gcgatcagaa
     8761 atatgaattg cttcgtttct ggctcctggg cacatggatg gcgaaacaga tgaataaacg
     8821 ctttttactg gccaatctgg tcccggccga caaggaacaa agcatactct gggaattcgg
     8881 cacacatatc aaggaacccg atgccgggat tttcacacgc attacctggg aagacattta
     8941 caatgcgatt gtaaaaacaa atctatcaaa cagcgacacc gacaagatgc ttcattattt
     9001 caggaataaa acactgggat atgactcttc gaaaaattta atcaaagcat ttaaaatctg
     9061 atcatatgaa actaattagt tattacaacg aagatctcga aaaaattctg actgctgtat
     9121 tcggcattgt tggaattgct gctattttta tcaaccttga tattaaaggc tatggaaacg
     9181 aaaactggct cgacgcaatc aaagacattg caggtctgat agtggtttta gccgttttta
     9241 tagcagctat ccgaatcagg cagaaatctg aaacacatta tgaaatggct cggaatgccc
     9301 tgcagcattt acaggccaaa catccgcaaa tccttatggg acctcgctac aacagagaag
     9361 gatatgatcc tgaaaaagga aaaggacttg agtatttgtt tgtgacaaat gagaatcgga
     9421 aatcaactct tcgtgcgaag ctaataccca ttcagccgtt agaagaagga tgcctctaca
     9481 tttatataca aacagccact cttgctgatg cattgaatta tggccgcggc aatgtgacgg
     9541 atgaagacgt ttcaaaagta aaagatgccg taaaagaagc tgtcaatacc attcttaacg
     9601 ctggttataa agggaagttt gagacccttc cggcggttaa agattctgta attgcaattg
     9661 attttaatga gtctgatatg cgtaaaaagc agtttgccaa agctgttgca gattgcaccg
     9721 aagccgcaac actaaaatta cttgaattca gaaaataaaa accgaagtag ttttcaaaat
     9781 cttcttcaaa cccgacattt catcgttagt ccgaaggact tacatggaat cgtcgggttt
     9841 tctttttgca atgattccat gtcgccacct acggttgcaa gactattata ttgaaaaacc
     9901 cctgcttctt ccttcctact tccttgcctg cctgttgaag acatgttcca tgttcaatat
     9961 tcaactccgc cacgctttgg cgtagtgacc ttctaccttt gtcagaaaac gataatgcca
    10021 tgatgaataa aaaagaactt tcagaaaaaa gaaaaatgct gcaaaacgcg cgcgtggagc
    10081 tgaaaaagga attcttcgga attgataaaa ttattgatga agtctgcagg gttgttgaat
    10141 cctgggtgct gtttcccgaa atgcaggaac gaccgctggt aattaatctg tgggggatga
    10201 ccggtgttgg aaaaactgcg ctgataaaaa gactatcgga actgattgga aaggccgaaa
    10261 tgttctttcg tttcgacatg ggagacaact cttcgaactg gactatccgc gaaaccctca
    10321 ctgatgttct cgaaaaagaa aacagcagac caatgattct ggtgtttgat gaatttcaga
    10381 atgccagttc tacaggaaaa gatgagcgcg aaagccgggc aacaaggctg gtctgggata
    10441 ttcttgacaa tggacgcttc aattctaata caatacggtt ttccaatttt gagaaattgc
    10501 aacgtttgat ttatgcttcg caatttatgc tcagcaaagg agtgaccgtt aaaaacggat
    10561 ttgttacctc aggaatgtca attttcagaa actactctgt cctgaagtcc tcggaatttt
    10621 acagacatgc ggaaaaaagc tacaaacatg aaagggggaa taaatttcca tttgtcgcag
    10681 attcctatct cgatgattta ctggactttc tctggccaaa attcaaaacg gaatttgaaa
    10741 tacgtgaata tcttgacact ctgaatggca cacagactgt agattttctt acagagatct
    10801 ttcataaatc aataacccca aaaacatttg attgcagcaa atcagtcatt tttgtcatcg
    10861 gaaatcttga tgacgcctat tctatgagcg ggaattacaa ccctgatatg agcgccgatg
    10921 attttcataa ggaatcgctg aaaattacac gctctgatat aaaaatggtt ctgtcatggt
    10981 cattccgaca tgagcagatt gcgcggcttg gaaatattca tctggtttac ccatcattaa
    11041 gcagcaacgc atacaagcga atcatcgaag agtcgctgaa gaaaatcagt cgcaattatc
    11101 tgaacaaaca cgatatggat tttgttttca gtaaatgcat tcatgaaatg atttttgcag
    11161 aaggtgtcac gcctgcacag ggtgtcaggc ccattcagtc aaccattaat caacttatcg
    11221 tatcgaacat tggccaggta atcaaccaga tgtcgaagtt gcgcaagaag ccggataagg
    11281 ccgttttcga tgtctgcaat gaaaccgttg tagtgaactt catgaaaggt aaagtgcaat
    11341 gcggatcaat gaagtatgat gtagatctgg aaaatgcgcg gcttcggaaa atcaaacacg
    11401 atgataatca ggcgatagtt gccgtgcatg aggcaggcca tgcggttatt gacattgtgt
    11461 tgcttaaggt aattcctgat cgtattttgt cacgcactgc cgatgcttct gtcaatggct
    11521 ttgtcgatgt cagcaagaag ccggaatttc attccgtttc aattatccaa aatgaaattg
    11581 tttcactttt tggcggtctt gctgcagaag aaatcatttt cggtcatgag catgttacgg
    11641 ctgggtccgg ttctgatatt tcacaggcaa cacgtcgcgc cacagccatg atccgaaacc
    11701 gcggcatggg ctcttttccg ggcatggtca accttccaca gccgatactg aatgagggct
    11761 atcttgatct tgacggtgca cttaacaatg aagtaaagtc gctgttgcag gaatcgtatt
    11821 cggctgcatt gaaaatcatc actgaaaatc agtcactatt gcttgaactt gcttctatat
    11881 tgaatgccaa cgcctgtatc gacaaggaac aaatcagaca gttgttgtgt acacgctttc
    11941 cgcatctgtc tgcacaagcc accggcaata gttcttccgg tttctcatac cgccatcacc
    12001 ttctgcgcaa atgtgctgca gcgtcaacac aaaacccttc ttgttcattg aatcagatgc
    12061 ctgcgatggt gatgaatatg gagaaaactg aaaaagaaat tcagccggaa taatattttt
    12121 ggtaatttgc gaaaaaatat gactatgaac acatttgaag aaaagtatct gtacaaaatc
    12181 aaagatttat tcgaaaagta taaggggaaa atgagagatc cggagttgaa tgaaattctt
    12241 gacaaagggt ttgtatcttc taaaccaaat ttgaatgcaa agattctgtt gtgcgggata
    12301 aatccgtccg acaatggaaa agaagaaaaa aactttcatt ttcatgattt agcgagtgcg
    12361 ccttatttca gaacattaca tgatgttgta agggagtgtg aaaacaagaa ctcgattgac
    12421 tatctcgatt tattttgttt tcgaaataca aatcagtatg acatctggca atttgcaaaa
    12481 gattctgtgg gtctcagatt tgttgtcgat catttgattc ttacgcagga aatcattgaa
    12541 gacattcaac caaaggttat tctggtattt aataaaacag ctgcaacatt ttttggatat
    12601 catgcagccc cagatgatgc aaatccgact aaccaaaaga tttggatggg ttataagtta
    12661 gaagacagct caatgactga caatatgtat tcagcacaga tgaaatatat tacagggctc
    12721 catgaaggcc gcatgaatac agaattgaaa gaaacatctt tacggggttc caaaatattt
    12781 tttggctcat atatgagata tcttaaaaat gagaagaaag aggagcttcg gaaattgatt
    12841 gcgtcgttat cgaaatttga gtaaataaaa aattctgtcc cttccttgtt gaacagattg
    12901 tatttgaatt tgtcaaatgc acgcatccct catcaattct ccaacatcgc cagaatctct
    12961 cccatccgtt ttgccgtcag aattttcggg tgtgaaaccg gctctgtttc tgcttcgtgc
    13021 tgccaggtga ctgaatacgg aatgtgtatg gcggtgcctc ctatggctac aaccggcaat
    13081 acgtctgatt tcatggagtt gccaatcatc aggaactctt ctggtcttgc attcagtttc
    13141 atcaacaatt tctggtaatc tgcttcggtt ttgtcgctca tgatttcaat gtgatgaaac
    13201 attttttcaa tgcctgattt tgacagcttt cgctgctgat cgagcaaatc acctttggtg
    13261 gcaatcacca tcgtatatcg ggagtgtaaa tccgatagca cagtgagaac atctccaagc
    13321 aatttaacgg gcttgtccag caatgctttc cccaatgaaa taatgccgga aacaatattt
    13381 ccacctgcct tgccgtcgct cagatgcatt gctgtttcaa tcatcgacaa cgtgaatcct
    13441 tttgccccaa agccatacaa tcctatattc tgtttttcag tttgcaataa aacttctgaa
    13501 acccgctccg gtgttgcata ttcatgcatc attgcgcaaa acattttttc tgtttcctga
    13561 taatacggtt cgttcaccca tagcgtatca tccgcatcaa aagcaataac acttatcttt
    13621 ttcattctac actttctttt ttattgactt gcttaattca ttttcgccaa ctccggccgg
    13681 attgatttcg gactccggat catcgttgtc cgcatcaggt gtatatttca agagtgctac
    13741 cccatcagga agggtttcat ctgaaatcag aaacaaggct tcgtcttcgt caaaacatga
    13801 acccagggca tacactgttt gatactccga ggccgggaaa tccattttta ttgcggcatc
    13861 cagatctatt tctccgtcaa tctcatcaaa tccaaggcgg tagttattct ccattaaaaa
    13921 cgagttctgc agcaatttca taaaccgaaa gtttccatga acggaattgg ctttgaaagg
    13981 tttgtttgcc tcgtagaaat caacctccca ctcaccaata agcaatacaa acggagtccg
    14041 cgtttctata ttaacgccgt cgggacggta atctttcagt tcaaaatgat aaatcttttc
    14101 aaccatttca aaaatgattt tactcaaagt tagggaattt ttgattgttt caaatacgct
    14161 ggcaaattaa cggccagaag ggtggcactc tttgctccga aatagtggca ctgtttgggc
    14221 cgaaataagt ggcgccgttt gtggcggaat agtaatcata tcagatatgt ccgaaatgac
    14281 acacttattt gaacacactc ccggaaatat ttggaattac tcaaaatcac ttt
//
LOCUS       DAOPKE010000069        14300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4444_length_14300_cov_12.444015, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000069 DAOPKE010000000
VERSION     DAOPKE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_14300_cov_12.444015"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PKY63_09170"
     CDS             complement(<1..1433)
                     /locus_tag="PKY63_09170"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPF00823.1"
                     /translation="MEIKLGLYRRRTVVGTNVYLVDSPDSLPAGLFSKEEKTFMKSRI
                     RKDKAKFVYFNRLPNLDIIAFLPEKIRQESDVEKLRRLGNDVLKLSNKEKIEEISLDV
                     YLNEPALAQYFLEGMLLGNYEFVKYKTEKEPKKILKSVLIRQALVEHVHIFELMRMQK
                     AVSFARDLVNEIPSELTSVTFPKRVSDFLKEVPVKVEVLSKQKIIALKMGGLLGVNRG
                     SSDEPTFTVMEYKPKIHVNKKPYVFVGKGVMYDTGGLNLKPGASMADMKCDMSGGAAV
                     AGALYLIASMQLPVHVIGLVPATDNRPGGNAYAPGDVLTMMNGKTVEVQNTDAEGRLI
                     LADALAYAQKYDPALVVDIATLTGAAHAAIGHYAIVGMEADASGYFERLHGAAEEVFE
                     RIVEFPFWDEYAELLKSSVADMANIGGKYAGAITAGKFLEKFTDYPYIHLDIAGPAFM
                     ETAWNYWPQGGTGVGVRLFYQFIKEEA"
     gene            1467..2087
                     /locus_tag="PKY63_09175"
     CDS             1467..2087
                     /locus_tag="PKY63_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00824.1"
                     /translation="MFIRNHNLIFCQYPVLVDYNNMNISTKVIISDLCNLKHILIVKE
                     REKAMRDYRNQQNSRTPVLMQRAFRIAFPVFAGLFVNVLMLLVLFLIAGTKITMSQEF
                     KHFMLYYSHLCVVIAFLFQAFIVFPVFCKYHFKSMTEKTIGSIAVFLVAASGSFLFAF
                     IVSTPSDKAPDFVINAVFGFIFTLVYLLTNILVVEKFCINENREEE"
     gene            2163..3101
                     /locus_tag="PKY63_09180"
     CDS             2163..3101
                     /locus_tag="PKY63_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HPF00825.1"
                     /translation="MKKTVLLFAFLCFSLMQINTSTAQSAQISGLDFENWNQNYYNIM
                     FPNNYYWENLPTNVWASSNAATTIVNDFCCERTTDCHGGSFAAHLETKSIFGVPAAGN
                     LFTGEFIPDGFSSQALRGVPFTDKPESIKGYYKYTPANYNNGSATVPDTCAIYAILSY
                     WDGAQRVEIARAELYTSNVVISYTVFNLDFNYISTETPDTIAVVFASSKYGDLFEGGI
                     GSTLLIDDVELVYPVGYAEKNSFDRAWSDGDFWNFSFRTNANRTIQLFDATGRLIVSA
                     SSSEKLFSTSAQEFPHGVYLYQISEGHNMYSGKLIK"
     gene            3098..3844
                     /locus_tag="PKY63_09185"
     CDS             3098..3844
                     /locus_tag="PKY63_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF00826.1"
                     /translation="MMREFLTIFFILLLMNYSYSQNLTIGPKLGFNVGAPLPLGNIPD
                     GAKGTPLACHNLGVFFNYKISEKFSIQAELLYNRKGAQFTTPLDSMPYTDRMQHPLYP
                     DIWLDVETFFNGTAEGAFDNYYFEIPLLLSYKIGPSKWSLMTGGYYGWLAQTETHATA
                     VGYVGYDPAQREEVLDFAENTRKYDYGALIGTTFKASDRINIDIRISFGMKSVFIDEY
                     KKIDYSLNNMFAQFTASYSFIPANWFGKKQ"
     gene            3841..4932
                     /gene="mqnC"
                     /locus_tag="PKY63_09190"
     CDS             3841..4932
                     /gene="mqnC"
                     /locus_tag="PKY63_09190"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HPF00827.1"
                     /translation="MIKDLSTLLSKPISDETLSSEEAMSVYKNTPLPQLISAAYNLRM
                     KLHPENEVSWIIDRNVNISNVCITGCRFCNFSCSKNSPSAYVTSRDEYRKKINELYAL
                     GGNQLLLQGGLNPALDLQFHCDLFRWMKSEFPKLKLHALSPAEVDFMARTEKLSHEET
                     LEMLINAGLDSLPGAGAEILSDRVRKIVSPSKISADGWIEVMRVAHKLGLVTSATMMF
                     GHVETLEERIEHMIRIRELQKEKPENAPGFLSFIPWPFYGKDTALEKAGLVDKSVTPV
                     EYIRLIATARLVLNNIPNIQASWLTVGKTTASVCLYAGANDLGSIMIEENVVSSAGAS
                     YRMNADEMLQLISDSGFEPRRRNQDFTDV"
     gene            5139..6539
                     /gene="xseA"
                     /locus_tag="PKY63_09195"
     CDS             5139..6539
                     /gene="xseA"
                     /locus_tag="PKY63_09195"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPF00828.1"
                     /translation="MPAPNNTNPFLTLSEVLSQVRVVVAREFALPLWIKADMLKLNHY
                     SASGHAFPELVEKVNGKITAKCQSMIWADDLQRIATNFRQITGKQLTDGIEILFRAEI
                     RFHEQYGMRLRIIDIDAQYTLGQMAMERKNTIEKLKKEGLYFLNKQVVLSEVPERLAI
                     ISAATSRGLHDLKNIVFSAGAPFKIKMTLFTAVLQGARGAETIGLRIQQLSKRSNEFD
                     AVLIIRGGGDDTGMDCYDNYALARAVCECPLPVITGIGHSTNETVTEMVAHTNKITPT
                     DVAYFIIGKFSEQLSKLQDLTSQLSAQTLMSIATEEQILQDMAQSVSDIARSEFQQQK
                     DFLLQAGNIIQQKSFQRMASAKTILEANGADLKTLSQKILLNQQHGIEMLKSALNQHF
                     QNIVRKETSTLDILESKIKMLDPQNILRRGYSITLHQGKIVKSTKALKEGNEIETILS
                     NGRVKSIVQKNKKDTI"
     gene            6536..6754
                     /gene="xseB"
                     /locus_tag="PKY63_09200"
     CDS             6536..6754
                     /gene="xseB"
                     /locus_tag="PKY63_09200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPF00829.1"
                     /translation="MSEPKYSEAMAELEEIVNAIENDTPDPDELIVKVKRAAELIQFC
                     RKRLLNTETEINTVLAKLKEMSDGGESQ"
     gene            7174..7962
                     /locus_tag="PKY63_09205"
     CDS             7174..7962
                     /locus_tag="PKY63_09205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPF00830.1"
                     /translation="MKKDNAFKRLLHKLRIPYRLVIMNDSTFEEKFSLKLSRLNVFVV
                     FALITFLLITLTVFLIAFTSLREYIPGYGNVEEREMVYELSLKTDSLEQALIARDVYF
                     RNFFAVLTDADSNLLISNYSNYSEGGSSSNVSENTDATGLYFIPPIEGTVVRKFSTAN
                     RHYAVDLNAPKGSMISSIANGTVLFSDWTLSGGNTLIILHPGKVISVYMHTLSLFVKQ
                     GETVKAGDPVAIIGNSGENSSGPHLHFELWIDQQAVNPENYITF"
     gene            7962..9116
                     /locus_tag="PKY63_09210"
     CDS             7962..9116
                     /locus_tag="PKY63_09210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPF00831.1"
                     /translation="MKQRLAILGSTGSIGMQTLDVCRCFPEQFEVEVLTAGKQAAELV
                     KQALEFNPNMVVIADESQYHFVSDALKNTDIKVFCGSKSIADAATVESVDTVVLALVG
                     IAGLEPAYAALKAGKKLALANKEVLVVAGELIAKTAIENRAAIIPIDSEHSAIMQCLQ
                     GESSAAIEKIILTASGGPFRGFKKDQLENVTLKQALAHPVWSMGNKITIDSATMMNKG
                     FEVIEARWLFNVPAEKIDVIIHPQSIIHSMVQFIDGSVKAQMSRPDMRLPIMYALTWP
                     ERMAAPFQTGGINDIFSLTFEKADTETFRHLSLAYEAIKNGGGAPCVLNASNEVAVEL
                     FLNDKISFTGMQKMSETAMSHISAGVPGSIADYLALDNETRIYCKTLNIK"
     gene            9118..10440
                     /gene="rseP"
                     /locus_tag="PKY63_09215"
     CDS             9118..10440
                     /gene="rseP"
                     /locus_tag="PKY63_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPF00832.1"
                     /translation="MEIFIKIVQLLLSLSILVILHEFGHYITARMFKTRVEKFYLFFD
                     PWFSLFKFKKGDTEYGIGWLPLGGYVKISGMIDESMDKEQMKQPAQPWEFRSKKAWQR
                     FIIMVAGVVMNVLLAMLIYILMLSAWGEKYLPNAELKDGIAVDSLAMQTGLRTGDKVL
                     KVDDIVIEDFFDIVPNMLLENAKTLTIVRNDSEQVLDIPSDFVKKMIDSERANMIMPR
                     MPMIIDSIVPNSVNANSKIKKGDRVIGVNGKLIHYHDEFKSEVEKYKNQEITIRVLRG
                     TEDLTYPVKVNDKGQLEVMIVTDLNRFFNVATKDYTFWEAIPAGISKAGTKISEYWKQ
                     LKLMFNRDVRAYENVGGFITMGKIFPGVWDWQAFWNLTAFLSLMLAVLNILPIPALDG
                     GHVMFVLIEMITGRKPSEKVLEIAQYIGFGLLLTLLVWANLNDILRLF"
     gene            10562..>11085
                     /locus_tag="PKY63_09220"
     CDS             10562..>11085
                     /locus_tag="PKY63_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00833.1"
                     /translation="MKLKPKSIFFAKSGLMFLTVLLLNTPIVFAQYGLSLQNGSATPT
                     TGTNCGNSQSITLYRNGSTTYSGCPRFRHFLEYKVGSGSWIAVGQWSGTGSGSYPASI
                     TVSKTIFSSDLTSWNPSGQTLYFRSRQEMYYTGLSGCIGTTTTTYSNTVTINYISPAT
                     ITSQPINSSVVEGN"
     assembly_gap    11086..11185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11186..13374
                     /locus_tag="PKY63_09225"
     CDS             <11186..13374
                     /locus_tag="PKY63_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00834.1"
                     /translation="PATITSQPINSSVVEGNNTSFSVTASNATSYQWQVSTDGGSTYN
                     NITAAGSNPVYSGYNTSTLTLTGVVAANNGYKYQCIVTGCNSVTSSQATLTVTSTCLA
                     PNVQASAATFTTLTSTSGTLSWTNGNGTGRVVYINTTNSFTAPTDGSSPTANTAYGGS
                     GQQCVYNGNGNSVTITGLTIGTTYYVRIYEFDCSGILYNASTASNNPTNFSIFECNYP
                     VVQATSFTSNPYSNGMIVGWTNGDGSNRIVFINTTNSFTAPVDGNTYTANAVYSGSGQ
                     QCVYNGTGSAVDVSGLNSSTTYYFAVYEFNSSGSCYTSSPLSGNATTTSTASTYIITS
                     IDNGAGYTGTFNGNTNSTIYLCYGSSVCIQTNIPLSLGDYSKRFQTSSDAGVTDPWFT
                     WAGTTYCSPSSGVRYVRGCLWDASLGVMPGSETPWLKIVHGPESVADPTNIATTNITP
                     TSFTANWTSSATVCAGGGLGIIHRIDVATDASFSNMLTGYPKDVTSCITNGPTLCSYD
                     VTGLTTGITYYWRVKAMSYHVGTNNGDLYCGVNSNYVPTSFNLPVELTDFSTKCNVAS
                     VTLSWSTATETNNDYFTIEKSINAEDWVPVGIVDGAGNSNELINYEFEDMEPMSGTSY
                     YRLKQTDFDGKFEYFGPVTVNCDEGANGIIAYPNPAQENVLVVGSQDVAAEIYLCDLT
                     GRIITSYSCSRLSEPFSIDLQNILPGIYLIRVDSQNNSEYFRVVKE"
     gene            14078..14251
                     /locus_tag="PKY63_09230"
     CDS             14078..14251
                     /locus_tag="PKY63_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00835.1"
                     /translation="MNGTFPTMGGNMLFVLCIFLFGFLIPLIVYFIVFHKNQKQLETE
                     IGTFIQQKHGLKV"
BASE COUNT         4237 a         3085 c         2980 g         3898 t
ORIGIN      
        1 tccgcttctt cttttatgaa ctgatagaaa agccgcactc cgacgccggt tccaccctgt
       61 ggccagtagt tccaggcagt ttccataaag gcagggcctg cgatgtcgag atggatataa
      121 ggataatcgg taaacttttc aagaaatttt ccagccgtaa ttgcccctgc atatttgcca
      181 ccgatattgg ccatgtcggc cacacttgat ttgagcaatt cggcatattc atcccagaaa
      241 ggaaactcaa caatgcgttc aaaaacctct tcggcagcgc catgcaaacg ttcgaaatat
      301 cccgaagcat cggcttccat tccaacgatg gcataatgtc caatggctgc atgagcggct
      361 ccggtgagtg ttgcaatatc aacaaccaga gcaggatcat atttttgtgc ataggccagt
      421 gcgtcagcga gaatgaggcg gccttcggca tcggtattct gtacttcgac tgtttttcca
      481 ttcatcatgg tcaatacatc gccgggcgca taggcattgc ctcccgggcg gttatcagta
      541 gcagggacaa gtccaattac atgcaccggg agctgcatag aagcaatcag atacaaggca
      601 cctgcaacag cagcgccgcc agacatgtcg catttcatat cggccatgga agcacccggt
      661 ttcagattca acccacctgt atcgtacatt acgcctttac caacaaaaac atatggcttt
      721 ttgtttacat gtatttttgg cttatattcc atgacagtaa aagtcggctc atcgctgctg
      781 ccgcgattca cacccagcaa accacccatt ttaagggcaa tgattttttg tttggaaaga
      841 acttcaacct tgacaggaac ttctttcaga aaatctgaaa cccgttttgg aaaagtaaca
      901 gaagtaagtt cagagggaat ttcattgaca agatcgcgcg caaacgaaac agctttctgc
      961 atgcgcatca gttcgaaaat atgaacatgt tccaccaacg cctgacgtat taaaacagac
     1021 ttaagaattt tctttggttc tttttctgtt ttatatttca caaactcata atttccaagc
     1081 agcatccctt cgaggaaata ctgagccaag gcgggttcat taagataaac atcgagtgaa
     1141 atttcttcaa ttttttcctt attggacaat ttcagcacat catttccgag ccggcgcaat
     1201 ttctcaacat ctgattcctg tcttattttc tcgggaagaa atgcaataat gtcgagattc
     1261 ggcaaacggt taaaataaac aaacttcgct ttatccttgc ggatgcgtga tttcataaat
     1321 gttttttctt ctttcgaaaa aagtcctgca ggcaaactgt caggagaatc gacaagataa
     1381 acatttgttc caacaactgt acggcgacga taaagaccga gtttaatttc cataatcagt
     1441 attttacaca aaaatactaa ataataatgt tcatcagaaa ccacaatctt atcttttgcc
     1501 aatatcctgt tcttgtcgat tataacaata tgaatatcag tacaaaagta attattagcg
     1561 acctttgcaa ccttaaacac atactgattg tcaaagaaag agagaaggcg atgagagatt
     1621 acagaaatca acagaattca agaacaccgg ttttgatgca aagagcattc aggattgcat
     1681 ttcctgtttt tgcaggcctg tttgtcaatg tactgatgtt acttgtcctg tttctgattg
     1741 ccggcacaaa aatcaccatg tcgcaggaat tcaaacattt catgctttat tattcacacc
     1801 tttgtgtagt aatagctttt ttgtttcagg catttattgt ttttccggtt ttctgcaaat
     1861 atcatttcaa atcaatgaca gaaaaaacca tcggcagcat tgcagttttt cttgttgctg
     1921 catcagggtc gtttcttttt gcatttattg tttcaacgcc atccgacaaa gctccggatt
     1981 ttgttatcaa tgctgttttc ggattcatct ttaccttggt ttatcttctc accaacattc
     2041 tggttgtaga aaaattctgc atcaacgaaa atcgcgaaga agaataaaca gctgaatttc
     2101 aaagaaaacc aacaccattt atctgttttt ataaatgtta tttatctttg cgaaaaaaat
     2161 tcatgaagaa aaccgttctg ttatttgcat ttctttgctt ctcgctgatg caaataaata
     2221 cttcaacagc acaaagcgca cagatcagcg gactggattt tgaaaactgg aatcagaatt
     2281 attacaacat tatgttccca aataattatt attgggaaaa tctgcccacc aatgtatggg
     2341 ccagttcaaa tgctgcaaca accattgtaa acgatttttg ctgtgagcgt acgaccgact
     2401 gtcatggcgg tagtttcgca gcacatcttg aaacaaaaag tatttttggc gtacccgctg
     2461 caggaaatct ctttactggg gaatttattc cggatggatt cagctcgcag gcactacgcg
     2521 gagttccttt tacagacaag ccagagtcca tcaaaggata ttacaaatat actcccgcca
     2581 actacaacaa tggaagcgct actgttccgg acacatgcgc catttacgcc atcctgtctt
     2641 attgggacgg cgcacaaaga gtagaaattg caagagctga attgtacaca tccaatgtcg
     2701 taatttctta cacagtattt aatctcgatt tcaattatat ttcgacagaa acacccgata
     2761 ccattgctgt tgtttttgca tcgagcaaat atggcgatct gtttgaaggc ggtattggaa
     2821 gcacgcttct gattgatgat gtagagctgg tttatccggt tggctatgcc gaaaaaaatt
     2881 cgtttgaccg cgcctggtca gatggcgatt tctggaattt cagcttccgc accaatgcaa
     2941 acagaacgat tcagctgttt gatgccacag gtcgtctgat tgtttccgct tcatcttctg
     3001 aaaaattatt ttccacttca gctcaggaat ttcctcatgg agtgtatctc tatcaaataa
     3061 gcgaaggcca taacatgtat agtggaaaac ttataaaatg atgcgtgaat ttctgaccat
     3121 atttttcatt ctactgctga tgaattattc atattcacag aatctcacca ttggtccaaa
     3181 actgggattc aacgttggag caccacttcc tttgggcaac attcctgacg gcgccaaagg
     3241 tacaccactg gcctgtcata acctcggtgt gtttttcaat tataaaatct ctgaaaaatt
     3301 cagtattcag gcagaattat tatacaacag aaaaggtgca cagttcacta ctccacttga
     3361 cagcatgcca tataccgatc ggatgcaaca tcccttatat ccagacatct ggctcgatgt
     3421 tgagacattc ttcaatggta cagcagaagg cgctttcgat aattactatt ttgagattcc
     3481 attgttgttg tcttacaaaa tcggaccttc gaaatggtcg ctgatgaccg gcggatatta
     3541 tggttggctt gctcaaaccg aaacacatgc aactgctgtc ggctatgtcg gatacgatcc
     3601 ggcacaaaga gaagaagtcc ttgattttgc agaaaacaca cgcaagtacg attatggagc
     3661 actgattgga acaaccttta aagcaagtga ccgaatcaac atcgacattc gtatttcatt
     3721 tggaatgaaa tctgttttta ttgacgaata taaaaaaata gactattcgc tcaataatat
     3781 gtttgctcag tttaccgcca gttattcatt tatacctgcc aactggtttg gcaaaaaaca
     3841 atgatcaaag acctctctac ccttctctcc aaaccaattt cggacgaaac actttcttcc
     3901 gaagaagcaa tgtctgtgta taaaaatact ccattacctc aactaatttc ggctgcgtat
     3961 aatctacgca tgaagctgca tcccgagaat gaagtgagct ggattatcga ccgcaacgta
     4021 aatatatcaa atgtatgcat taccggatgt cgcttttgca acttcagttg ttcgaaaaac
     4081 agtccatcag catatgttac ctcacgcgat gaatacagaa aaaaaataaa tgaattgtat
     4141 gcgcttggag gaaatcaatt gttattgcag ggtgggctga atccagcgct ggatctgcag
     4201 tttcattgcg acctttttcg ctggatgaaa tctgaatttc caaaattaaa actacatgct
     4261 ttgagtcctg cagaagtgga ttttatggca cgcacagaaa agttatcgca tgaagaaaca
     4321 ctggaaatgc tcataaatgc aggccttgac agtcttccgg gtgcaggtgc ggaaatacta
     4381 tccgatcgcg tacgtaaaat agtttcaccg tccaaaatca gtgctgatgg ttggattgaa
     4441 gtaatgcgtg tagcacacaa acttggattg gtaacctcag ccaccatgat gtttggtcat
     4501 gttgaaacat tggaagaacg aattgagcat atgattcgta ttcgtgaact gcaaaaggaa
     4561 aaaccggaaa atgcacctgg ttttttatca ttcattccat ggccgtttta cggaaaagat
     4621 acagcattgg aaaaagctgg gctagtggat aaaagtgtca ctccggttga atacatccgt
     4681 ctgattgcca cagccagact ggttttgaac aatattccaa acatacaggc ctcgtggttg
     4741 acggttggaa aaacaacggc ctccgtatgt ctttatgcgg gagccaatga tctcggcagt
     4801 atcatgattg aagagaatgt cgtttcatcg gctggtgctt cgtaccgcat gaatgctgat
     4861 gaaatgttgc agctcatcag cgattcggga tttgagccgc gccgaaggaa tcaggatttt
     4921 acggatgtgt gaggcacaaa gcacaactat tcacaagtaa aaagttgaaa gtttataaag
     4981 tttataaagg tgccgttgga gcttacggca caacacttcc cgagccatca ggttatcaag
     5041 tttaaagttt gtaaagttta tcacagcatc tttctacttt aaaactttat gaactttatg
     5101 aactccgaat ttcctatctt tacaacaaaa atcaattcat gcctgcaccc aataacacca
     5161 atccttttct cacgctgagc gaagttctat cgcaggtgcg ggtggtggtt gcccgcgagt
     5221 ttgcgctacc gttgtggatt aaagccgata tgctgaagct caatcattat tcagcatcgg
     5281 gccacgcgtt tccggaattg gttgaaaaag tgaatggcaa aattacggca aaatgccaat
     5341 ccatgatctg ggccgatgat ctgcagcgga ttgcaaccaa ttttcgacaa ataaccggaa
     5401 aacaactaac tgatgggatt gaaattcttt tccgtgcaga aatccgtttt cacgagcaat
     5461 acggcatgcg cctccgcatc atcgatatcg atgcacaata tacattaggg caaatggcga
     5521 tggaacgcaa aaacaccatc gagaagctca aaaaagaagg attgtatttt ctgaacaaac
     5581 aagttgtttt atccgaagtt ccggaacggt tggctattat cagtgcggct accagcaggg
     5641 gattgcacga tttgaaaaac attgtttttt cggccggagc tccatttaaa atcaaaatga
     5701 ctttattcac tgcagtgctg caaggcgctc gaggtgcaga aaccattggt ctccggatac
     5761 aacaactctc caaacgcagc aacgaattcg atgctgttct gattatccgc ggcggcggcg
     5821 atgacacagg aatggattgc tacgataatt atgcattagc ccgagcagta tgcgaatgtc
     5881 ctctgccggt cattaccgga atcggccatt caaccaacga aacggttact gaaatggttg
     5941 cgcataccaa taaaataacc ccaaccgatg tagcgtattt tattatcgga aaattctcgg
     6001 agcaactgtc gaaactgcag gaccttactt cgcaactcag cgctcaaaca ctgatgtcca
     6061 tagcaacaga agaacaaatt ctgcaggata tggcacaatc tgtttctgat atagcacgaa
     6121 gcgaatttca gcaacagaaa gattttttac tgcaggccgg caacattatt caacaaaaat
     6181 catttcagag aatggcatcg gccaaaacca tacttgaagc aaacggagct gatttaaaaa
     6241 ccttatctca aaaaatactt ctcaaccagc aacacggaat cgagatgcta aaatccgcac
     6301 tgaatcagca ttttcaaaat attgtgcgca aagagacatc aacacttgac atccttgaaa
     6361 gcaaaataaa aatgctggat ccgcaaaaca ttctacgtcg cggttattcc atcacgttgc
     6421 atcagggaaa aattgtaaaa agcaccaaag ctttgaaaga aggaaatgaa attgaaacaa
     6481 ttctttccaa cggacgcgtg aaatccattg ttcaaaaaaa taaaaaagat acaatatgag
     6541 tgaaccgaag tattccgaag ccatggcgga actcgaagaa attgtaaatg ccattgaaaa
     6601 cgacacaccg gatccggatg aattgattgt aaaagtgaag cgggctgccg aactgattca
     6661 attctgccgc aaacgtttgc tgaacaccga aacggagatc aacaccgttt tggccaagct
     6721 gaaagaaatg tccgatggtg gcgaaagcca gtaaaaatta taagcatcat tttcgggcaa
     6781 ccaccgctgt aatgttgagc aacgccactg tcatcctgag cccccccaac tgtcaaattg
     6841 agccctgcct gctgtcacac tgagccctgc gttccgcagg ataaacttcg tcgaagtgtc
     6901 aaaagcagga taaactccat cgaaatgtcg atagcggggt gaaactccgt cgaaggatgg
     6961 atagcagaat agtgttgaca gcgaaaatca acagaagcag tgtctaacgt ataataggag
     7021 atcccggttg tttccgaaaa gctgtatcgc agtagcgacc gggaactaag aaggatcttc
     7081 tgttgattaa agataaagca gttttgcaat aatgtttcgg gagtacccca taaccggaaa
     7141 tttttaacga tatttgcacc tcttattaag cggatgaaaa aagacaatgc attcaaacgt
     7201 ttgcttcaca agctgcggat tccgtaccgg cttgtcatta tgaacgacag tacttttgag
     7261 gagaaattct ccttaaaact gtcgcgttta aatgtatttg ttgtttttgc actgataaca
     7321 ttcctgctga taacattaac ggttttcctg attgccttca catcactgcg agagtatatt
     7381 ccagggtatg gaaatgtaga agaacgggaa atggtctatg aattatcgct taaaactgac
     7441 agtctggaac aagcgttaat tgcccgtgat gtatatttcc ggaatttctt tgcggtactt
     7501 accgatgccg attcaaattt gttgattagc aattattcaa actattccga aggcggcagc
     7561 agcagcaatg tttcagaaaa cactgatgcc accggattgt attttattcc acccattgaa
     7621 ggcactgtag tcagaaaatt ttcaacagcc aatcgtcatt acgctgttga cctcaacgca
     7681 cccaaaggca gcatgatcag cagtatagcg aatggcactg tattattttc cgactggact
     7741 ctttcgggag gaaatacgct catcattctg catccgggga aagtgatttc ggtgtatatg
     7801 cacaccctgt cgctctttgt aaaacagggt gaaaccgtaa aagccggtga tccggttgcc
     7861 attattggca attccggcga aaactccagc gggcctcatc tgcattttga attatggatt
     7921 gatcagcaag cagttaatcc tgaaaactat ataaccttct gatgaagcaa cgattagcca
     7981 tacttggttc taccggctcc atcggaatgc agacacttga tgtttgccgt tgttttccgg
     8041 aacaatttga agttgaagta ttgaccgccg ggaagcaagc agcagaactt gtaaagcaag
     8101 cacttgaatt caatcccaat atggtagtga tagccgatga atcacaatac cattttgttt
     8161 ccgacgctct taaaaacaca gacatcaaag ttttctgcgg cagtaaatcc attgccgatg
     8221 cagctaccgt tgaatcggtt gataccgttg tgcttgcact tgttggaatc gccggactgg
     8281 aacccgccta tgcagcattg aaagccggaa agaaactggc tcttgccaac aaggaagtgc
     8341 tggttgtcgc cggcgagctc attgcaaaaa cggccattga aaaccgtgcc gccatcattc
     8401 ccatcgacag tgaacattcg gccatcatgc aatgtctgca gggagaatca tcagcagcta
     8461 ttgaaaaaat catacttact gcatccggcg ggcctttccg gggattcaaa aaagaccaat
     8521 tggaaaatgt tacgctgaaa caagcgctgg ctcatccggt ctggagcatg gggaataaaa
     8581 tcactattga ctctgcaacc atgatgaaca aaggctttga ggtaatcgaa gcgcgatggt
     8641 tgttcaatgt gccggcagaa aaaattgatg taatcattca tccacaatcc attattcatt
     8701 caatggtgca gtttattgat ggctctgtaa aagcccagat gagccgtccc gacatgcgtt
     8761 tgcccatcat gtatgcgctc acctggcccg agcgtatggc agcgcctttt cagaccggtg
     8821 gcatcaacga tattttttcg ctgacttttg aaaaagccga taccgaaact ttccgtcatt
     8881 tatctctggc gtatgaggcc ataaaaaatg gtggtggtgc gccctgcgtg ctgaatgctt
     8941 cgaacgaagt cgcggtggag cttttcctta acgataaaat ctcattcacc ggcatgcaga
     9001 aaatgtcgga aaccgcaatg agtcacattt ctgcgggtgt gcccggcagc attgccgatt
     9061 atctggcgct cgacaacgaa acacgcattt attgcaaaac tctgaacatt aaataacatg
     9121 gaaatattca tcaaaatcgt tcaacttctt ctcagtcttt caattcttgt tatcctgcac
     9181 gaattcggac attacatcac tgcacggatg ttcaaaacaa gagtggaaaa attctatctg
     9241 tttttcgatc catggttttc gcttttcaaa ttcaaaaagg gtgatacaga atatggtatc
     9301 ggctggttac ctctgggcgg ctacgttaag atttcaggga tgattgatga gtcgatggat
     9361 aaagagcaaa tgaaacaacc ggctcaacca tgggaattcc gcagcaaaaa ggcgtggcag
     9421 cgttttatca ttatggttgc cggcgttgta atgaatgttc tgctggccat gctcatctat
     9481 attctgatgt tgtcggcctg gggcgaaaaa tatcttccaa atgccgaact gaaagatggc
     9541 attgccgtgg attctctggc catgcaaacc ggactgcgca ccggagacaa ggtattgaaa
     9601 gtcgatgaca ttgttattga agatttcttc gatattgttc cgaacatgct tctcgaaaat
     9661 gccaaaacac tcaccattgt acgcaatgat tcggagcagg tattggatat tccgagcgac
     9721 tttgtgaaga aaatgattga ttcggagcgg gccaatatga ttatgccccg tatgccaatg
     9781 attatcgaca gcattgttcc caactcggtc aacgctaact caaaaataaa aaaaggcgac
     9841 cgcgtgatcg gagtgaatgg gaaactgatt cattaccacg atgagtttaa atccgaagtg
     9901 gaaaaatata agaatcagga gattaccatt cgggttctgc gcggcaccga agacctgaca
     9961 taccccgtta aggtcaatga caaaggtcag cttgaagtga tgattgtgac tgatctgaac
    10021 cgctttttca atgtcgccac caaggattat acattctggg aagccattcc ggcgggaatc
    10081 agcaaggccg gaacaaagat ttcggaatac tggaaacagc ttaaactaat gtttaaccgc
    10141 gatgtgcgcg cctatgaaaa tgtgggcgga ttcattacca tgggcaagat tttcccgggc
    10201 gtatgggact ggcaagcctt ctggaacctg acagcctttc tgtcgctcat gctggcagtg
    10261 ttaaatattc tgcccattcc ggccctcgat ggtggccatg tgatgtttgt actcattgaa
    10321 atgataaccg gccgaaaacc ttcggagaag gtccttgaaa ttgcccaata cattggcttt
    10381 gggctcctgc tgacactttt ggtctgggca aacctgaacg acattctgcg gcttttttag
    10441 cccgttgtta accgtttgta acccgccttt tttgttaaag gtattgcgcg cgggggtggg
    10501 aggggtgtat atttgtggga ttgatttgaa atatcatttc cgcaaatgaa acaactctca
    10561 aatgaaattg aaacctaaaa gcattttttt tgcaaaatca ggcttaatgt ttctcactgt
    10621 tttattgttg aatactccaa ttgtatttgc tcaatatgga ttaagtctac aaaatggaag
    10681 tgcaacacct acaactggaa cgaattgtgg aaactcacag tctataacat tgtatagaaa
    10741 tggaagtaca acttactctg gctgtcctag attcaggcat tttcttgagt acaaagtcgg
    10801 ctcagggtcg tggatagcgg tcggacaatg gtctggaact ggatccggat cctacccggc
    10861 aagcattaca gtaagtaaaa cgattttttc ttcagatttg acatcttgga atccatctgg
    10921 gcaaacatta tattttagat ccagacaaga aatgtattac acaggacttt ctggatgtat
    10981 tggaaccacc accaccacat attcaaatac tgtaacaatt aattatattt ctccggcaac
    11041 aataacttcg cagccaatca acagcagtgt tgttgaagga aacaannnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnctccg gcaacaataa cttcgcagcc aatcaacagc
    11221 agtgttgttg aaggaaacaa cacatccttc agcgtcaccg cttcaaatgc aacgagctat
    11281 caatggcagg tttcaacgga tggtggatca acatataaca atataaccgc tgccggatct
    11341 aatcccgtgt attctggtta caacacctcc acactaacct tgaccggtgt tgttgcagca
    11401 aacaacggct acaaatacca atgtatcgtt acggggtgca attctgtaac gagctcacaa
    11461 gcgacattaa cagtcacttc gacctgcctt gccccaaatg tgcaggcaag tgctgcgact
    11521 tttacaacac ttacctcaac ttcaggaaca ctcagctgga caaatgggaa tggaacagga
    11581 agagtcgttt acatcaacac aacaaattcg ttcacagcac caacagatgg cagcagtcca
    11641 acagcaaata ccgcatacgg tggatctggt cagcaatgcg tttataacgg aaacgggaat
    11701 tctgttacta taaccggatt aactatcgga acgacatatt atgtccggat ttatgaattt
    11761 gactgctccg gaatattgta taacgcatca acagcatcaa ataacccaac aaatttctca
    11821 atatttgaat gtaattatcc ggtagtacaa gcaacatcat tcacatcaaa cccttattca
    11881 aatggaatga tagttggctg gacaaacgga gatggaagta atcgtattgt ttttatcaat
    11941 acaaccaatt ctttcactgc acctgtcgat ggtaatactt atactgcaaa tgcagtgtat
    12001 agtggatctg gtcaacaatg tgtctataac ggcaccggtt cagctgttga tgtttctgga
    12061 ctaaactctt caacgaccta ttattttgcc gtttatgaat tcaatagcag tgggtcttgt
    12121 tacacaagtt caccactatc cggaaacgcg acgacaacat ccacggcttc aacatacata
    12181 attacgagta ttgataatgg tgcaggttat accggaacgt tcaacggcaa taccaattca
    12241 acaatttatt tatgctatgg atccagtgtc tgtattcaaa caaacattcc tttgtcatta
    12301 ggagattata gcaaaagatt ccagacatcg agtgatgccg gagttactga tccatggttt
    12361 acttgggcag gaacaactta ctgcagtcct tcaagcggag tcagatatgt aagaggttgt
    12421 ctctgggatg cttcattagg tgttatgccg ggttctgaaa caccgtggct taaaattgtt
    12481 catggtccgg aatcggtagc agatcctaca aatatagcaa ccacaaacat tacaccgacc
    12541 agttttactg caaactggac atcctcggca acagtttgtg caggtggtgg tcttggcatc
    12601 attcacagaa ttgatgttgc tacagatgca tcattttcta acatgctgac cggatatcca
    12661 aaagatgtta cttcgtgcat taccaatggg ccaactttat gttcgtatga cgtgaccgga
    12721 ttgacaacgg gtattacata ttactggaga gttaaagcga tgtcctatca tgtcggaaca
    12781 aacaacggag atttatattg cggagtaaac agcaattatg ttccaaccag tttcaatctc
    12841 ccagttgaat taaccgattt ctcaacaaaa tgcaatgttg cttctgtaac cctctcctgg
    12901 tccaccgcga ccgaaaccaa caatgattat tttaccatag aaaaaagcat caatgccgaa
    12961 gactgggtgc ctgtcggaat cgttgatgga gcgggcaatt ccaatgagct gatcaattat
    13021 gaattcgaag atatggagcc aatgtcgggg acatcgtact accgcttgaa acagactgat
    13081 tttgatggga aatttgaata tttcggaccg gtaactgtca attgcgatga aggtgcgaat
    13141 ggaattatcg cttatccgaa tcccgcacag gaaaatgtgt tggtggtggg cagccaggat
    13201 gttgccgccg agatttatct gtgtgatctg acaggccgca tcattacatc atacagctgc
    13261 agccggcttt cagagccatt cagcattgac ctgcagaata ttcttccggg catatatctg
    13321 attcgtgttg attctcaaaa caactcggag tatttccggg tagtgaaaga atagctcctt
    13381 cctcaacctg agtgcctcgc ggagcgagct ctctgcgttg agggagtcac atactagcca
    13441 atgcactcag gtcgattaca ccgacgcaga gagattggca atgtctattg aagcgaggca
    13501 ctcaggttgt ggaggcagca gcgccaaacc acgatcaaac caccgatcgc aataaatatt
    13561 tgttatacca ttatgaaata tattttagac ggaagtgtct tagttaaaat gatgcccgat
    13621 gcaagaggtt tacctgacaa acaggcaggc atcacatgtc tgtagaaaaa tgcatatagc
    13681 acttacgacc tcagagaggt cgaatctaaa cggcttcctg cgcgaaagag cttgaaaaac
    13741 attcgactcc gctggagtcg tgttttgtcc tttatgtttt ctacagacat gcgactcctc
    13801 cggagtcatc caaatacacg gactattgta tattaccttt ctgtattatt gcatgataaa
    13861 aaatatttca gacatattta ttccttatct ttgaccaaac tctacaccta tgaaaatcaa
    13921 aattccagaa aaccaggtca cgttttagca gctcaaagct gatgttgaaa gccagtttcc
    13981 acagtacgcc tttcatgtaa aaacaaagaa ttttcttgtt gcccgcaaaa acggaagcac
    14041 cggcgccaat attcttttga gaaaatcgaa agtcgttgtg aacggcacat ttccaactat
    14101 gggtggcaac atgctttttg tgctttgtat tttcctgttt ggatttctga tacccctgat
    14161 tgtttatttc atcgtttttc acaagaacca gaaacagctt gaaaccgaaa tcggcacatt
    14221 tattcagcag aaacatgggc tgaaagtctg aactgaatta cacaaaaaaa gaggatttgc
    14281 aatgcagatc ctcttttttt
//
LOCUS       DAOPKE010000070        14247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4461_length_14247_cov_12.295025, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000070 DAOPKE010000000
VERSION     DAOPKE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4461_length_14247_cov_12.295025"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2831)
                     /locus_tag="PKY63_09235"
     CDS             complement(<1..2831)
                     /locus_tag="PKY63_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HPF00836.1"
                     /translation="MKTHIKLLTSFLTALAFFLLQSWATAQDSEILSIHKTGPVAAAP
                     GELITYIIEYSNVGTVEAGNVVIKDYLPAANMYTYVGSNPAGTLVGNVLTWDKTNIPE
                     LELLGTGIRQITVQIRAGVPGDGITGSTDGYYMPAASNIISNYTSIQSDVTNPPILGD
                     TTYTTVNQMCSVQINDASGVIKSATNTVLYYLMQVNNNGNIYDKFDLTVVNYACIGTD
                     FDPLLSRLLDMGGNEITQTPWISPFSTYQFLIELTAPIGSNPFHFSCHDVTATSVYCP
                     TTSDVGYLETEILGSPKYPLINISKVDSKDPVERGEEFTYTIFVFNSNDKYPAEDFKL
                     YETYDPNIEFVSAVPAPDAGTNNVWSLGTLGYGLDSAETIQVTVRVVDSPICSGTITN
                     MVECSYLNQSRYPTATAITNIFGHPDLAIEKTLVTVPIPAVIGGDVFYTLKYKNAGNC
                     VADNVVLKDFFDNAHLNPVSLNGGIVSGGMIQWNLGSLNPGDSGQIVYSMMVDPTATY
                     NPGVTLVRNYASIITPVPENEYNNNNAMAVFSITLLPDLQVYKSEPDTLFTGVPYTYT
                     ITIRNNGDHTASDISLADVLPASLQFVSASDGGVFDAGKITWPLITSLAANTDVIRTV
                     TVIPLCTAIPSVINEANVSCVTLELDYSNNYVAVQSVVVDSISPVITCIPPQEVTTND
                     ACTFVVPNIDWDAIASDNCGIESLSYIISGATSGSGNSLLGVAFNTGVSTVTWTVTDK
                     NGNISTCSFNVMVSDVEPPVFVNCPDGSEFVLNTDSGFNTYTHSGFAWDATASDNCGI
                     DSLYFVMTGATIGSGSSLDGVVFNMGETAVLWTAADLSGNTIYCEFTVVVNDNQNPEY
                     SFCLTGNNQSVNTDLGADTYTQPDNSWDAIAGDNDGIASLTYVLSGATTGSGTSLAGV
                     AFNIGTTTITFIAVDSSGNEALCVFDVLVT"
     gene            3582..>5653
                     /locus_tag="PKY63_09240"
     CDS             3582..>5653
                     /locus_tag="PKY63_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00837.1"
                     /translation="MKKIYLVLISLFLAATTSQAQLMLSWANTCGGANNDYVYSCVKD
                     TSGNIYTAGYFSGTVDFDSGAGTYNISSSGIDAFVMKTDSSGTLKWVTKLGSTGTDQA
                     ISLAFDNNMHLVIGGIFNGTVDFDPGAGTATRSSNGGDDFFVLKLDTSGQFIWAKTVG
                     GNSNDWINDICVDPFGTICIGGQFKSTNSVDFDPGTGTATSSTSGTWSGFVLKFDVDG
                     NYIWHKKFAGSSNVIVWKVLSDSLKNVYIDGTLAGTADFSPDTTLSSAGGTDIFILKL
                     DSAGGFTWINKISSPANLDARGFALYKNQSFYLTGFFEGTVDFNPRGGIYNLTSAGNT
                     DVFILNMDTAGNFIWAKKIGAANADGTYGAYINDAGQLLISGGFSSTVNFDPGLTDYT
                     ITSNGSGDVFVMNTDLNGNFNWVKSFGGVNMDYSLCVLDLDSVHPVVCGSYSGTADFN
                     PGPGVYSKTSNGLGDIFVVRLEPCVTAEGYDTIQACDSYTWINGVTYTYSISGISHTL
                     PGAAASGCDSIVHLNLSIYPSYFFTEKDTICAGDELFWRGYQLTTGGLHYDNLTTIHG
                     CDSVYELLLTVIQDQEVISIENICAGDSIFWRGMYFKTTGNYYDSVTHTSSCDTVFIL
                     NLTVNPTYFSSQSAGICSGESFIWHGNPYTTAGMYYDSLVTQHGCDSVFELNLVVHPV
                     YLNQINAE"
     assembly_gap    5654..5753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5754..6317
                     /locus_tag="PKY63_09245"
     CDS             <5754..6317
                     /locus_tag="PKY63_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00838.1"
                     /translation="GIYYDSLLTQNGCDSVFELDLVVFDLPNVDIISTDSFVCVYNTP
                     LNLAAIPAGGVWNGQGLSGNNFDPAAAGIGSWIITYHFADTNGCENADTITILVDACT
                     GLENEVFSGITLYPNPTSGQLSITFPAEDDYTLSFIDALGQIILTTKISAQKTVQIDL
                     KSFAPGVYMLKIENAEGFVLKRVVLER"
     gene            complement(6830..8074)
                     /locus_tag="PKY63_09250"
     CDS             complement(6830..8074)
                     /locus_tag="PKY63_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF00839.1"
                     /translation="MTAQFQKDSQYFKFCSYGFLKNLRFFDPFIFLFFLDKGLSFFEI
                     GLLISFREVMVYVMEIPAGIIADVLGRRKVMIFAFIAYIISFSVFFFANAFWLFMLSF
                     IFYGFGDAFRQGTHKSMIIDYLRLKGWKDQKTAYYGHTRSWSQIGSAVSSLVAAVIVF
                     FTGDYQSVFIFSTVPYFLNLIMMTTYPKELDGEIVRHKGSDLKENFRQVIRDLITTFK
                     NPTVLKTVGNLSFYTGFYKAIKDYLQPLLETIAVSMPLFLMLHEKQRSAILIGIIFFC
                     IHLTTAFASRSAGRFAKRFKHVAAPLNITLLSGFLFGLIAGICYSYDWFIAAAALFVI
                     VYAVENLRKPIGISYLTDKIDPTVLSSSLSFQSLAETLIAAAIAPVIGFVADRYSIGW
                     GLIAVSAFMLIPAVLVRIKEKV"
     gene            8519..8860
                     /locus_tag="PKY63_09255"
     CDS             8519..8860
                     /locus_tag="PKY63_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00840.1"
                     /translation="MKGGIIFILILMFVAFNNVQAQTENTKVTQKYEYSFSGKATTQQ
                     LEKVESELYRIETITQVKISYKQDSFKGLLFFSRTFYSGPGELTDDFNAARIKQILSE
                     AGLIPEEFKEL"
     gene            8875..13071
                     /locus_tag="PKY63_09260"
     CDS             8875..13071
                     /locus_tag="PKY63_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00841.1"
                     /translation="MKMSFIFGMIVCIAFSFASIETQAQSDGCSAATAIPITVNCSSP
                     VSGTTAGATQTIAGCVGTADDDVWYTFTATATSHIITVTPSSGMDAVVQLFSGDCAAL
                     ISMNCMDQGFTGEAETIYATGLTIGAVYKIRIYHYYTGYGSGTFTTCITTAPPAPSND
                     NCSGASLLNVNATCVSTAGTTVSATQSQPGCAGTADDDVWYRFVATNAVQTIQVTPSG
                     TADLVLELFSGSCSVLTSLYCVDNGFSGDAETISAVGLIPGTTYYVRTYDYYSGNGGT
                     TFDICIIGTPTPTPTNDEPCDAIQLPPVTSSCDYLSFTTTGATTTTSAPTPASCAGGS
                     SPMQGGFNNIPQPKDVWFAITVPSSGIISIMAKPGYGFSDAVMALYSGTCTSLTQIAC
                     SDDNNYPLTANDFKPFLLASGLTPGTTVYLRYWAFNGNTTGDFGFCVTTPINDACSTA
                     LYICDLNGYKGTTGEAYTPDRPGNMRGNAETNNPPLYTYTAGTCQGGIFGAGGVWGTG
                     APNCDVQINNNSWVRFTAANDSAVLNVDVYDCYSGTVAGIQMQVFSAGSACADFTPVS
                     NFEEGATHIEITARGLTVGSDYYLMIDGFAGDICNYTISAESGVQFPNITAAQDPICA
                     GNSTTLFGPPGATAYHWYPGNETTQNITVNPATTMTYNLEVTGVCGYRQYLDYTLDVN
                     QLPIATASSNSPECAGSTLNLFGSGGTIYQWTGPNGFSSTSASPSINNISSAAAGTYN
                     LTVTSAAGCSSSTTTTVLVNPNPTANAGVSQTIPFGTSTTLSGSASGGSGTYLYSWSP
                     AGLLVSSNIASPTTTNLTTTTVFTLTVTDQTTGCTSTAQTTVNVSGGALTVNALASAP
                     GVCAGNGITLTALASGGSGSYTYSWSSNPSGFSSSDDNPYHIPSGTASYTVTVSDGFN
                     SATSTINVIVYPQPTATASNTGPYCAGENIQLSASGGGTYSWVGPNGFSSSLANPVIT
                     SSAAVNNGTYTVIVTSAEGCTDSETTNVIINDIPVATANNNGPYCSGETVNLSASGGL
                     SYAWSGPAGFSSSAANPAITAATPAVSGTYSVVVSGTGGCTATASTSVLINPLPSVIC
                     SNGGPYCTGETIMLSASGASDYSWAGPAGFSSNSQTPEITNAQLSHTGTYSVVGTDLN
                     GCMNAATTTVNVNQSPEINISGETEVCQDGTAVLSANGANLYIWNTGATTASITIGGI
                     ASATYVVTGTTGTCSDIDSISVTVNPNPTANAGADTTIEEGQSIQLNGSGGGTYSWSP
                     PDGLSDPDIANPICNAEDTTVYILTVTNEFGCTDTDTVIVRVDGDCGSIYVPNAFSPN
                     GDGKNDVFGVMNRCLETLNLKVYNQWGNLVFETNDPNGRWDGTFSGTMSESGIYSYWF
                     DGSLSDGTLVNGQGNFVLIR"
     gene            13082..14101
                     /locus_tag="PKY63_09265"
     CDS             13082..14101
                     /locus_tag="PKY63_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPF00842.1"
                     /translation="MKTKKTMAFIMLLLSASAISNRVSAQDPHFSFSNAFIEYQSPGL
                     IGTQDNQMSFQMIYRNQWRNVGTAFQTYGLSGNSRVSKSTNTSFSVGGFIFNQSAFSY
                     SSLLAHVGAAANVKLSEGKILSVGIGSGTRYESLNPDDLMWTDQFNGYEYDPNIQHGE
                     YFDRQQKASFDLSAGISYKFMSEAPNIDRFSKSSGTISAGFWHLNQGKRLTLSDEQAD
                     IKMVMMTDLTFAMGEDKKAAFNPWILGMLQGSAYEFTVGSDIKFFLKQSDKYSVSRAI
                     FSIGGAYRLNDAAMARIMFDYANYGIGIAYEINTSSLKEASKGFGCFEIFIKYSPMSK
                     SFSLY"
BASE COUNT         4140 a         3177 c         2989 g         3841 t
ORIGIN      
        1 gttacaagta catcaaacac gcacagggcc tcgtttccgg aactatcaac ggctataaaa
       61 gtgatggtgg ttgtgccgat gttgaatgca acacccgcca gcgaagtacc gctgcctgtt
      121 gtggcgccgc tcaatacata agtcagactg gcaattccgt cgttgtcgcc ggcgatagcg
      181 tcccagctat tatcgggctg tgtgtaggta tcagcaccca aatcagtatt gacggactga
      241 ttgtttccgg tcagacagaa gctgtattca ggattttgat tgtcattgac aacaactgta
      301 aattcacaat aaatggtatt tccactcaaa tcggcagcag tccacaaaac cgctgtttcg
      361 cccatattga aaacaacacc atcaagactt gatccacttc caatagtggc accagtcatt
      421 acaaaatata atgaatcgat tccacaatta tccgaagctg ttgcatccca ggcaaatcca
      481 ctgtgagtat atgtattaaa gccggaatcg gtattcagaa caaattcact tccatcggga
      541 caattaacaa aaacaggagg ttcaacatca ctcaccataa cgttaaaaga gcaagtactg
      601 atatttccat ttttatcagt aacggtccag gttactgtgc taacacccgt attaaaagca
      661 acaccgagca aagaattacc gctgccagaa gttgcaccac tgatgatata ggaaagagat
      721 tcaataccgc agttatcact ggcaattgca tcccaatcaa tatttggaac gacgaaagta
      781 caggcatcat ttgttgtaac ttcctgcggc ggaatacatg taattacagg cgatatactg
      841 tcaacaacaa ccgattgcac agcgacataa ttattggaat agtccagttc caaagtgaca
      901 catgaaacat tggcttcgtt aataacagaa ggaattgcag tgcacaatgg aataaccgta
      961 acggttcgga taacatcggt attggctgcc agtgacgtaa taagcggcca ggtgattttc
     1021 ccggcatcga aaactccacc atcagaagct gatacaaatt gtaaggaagc cggaagcaca
     1081 tcagccagag aaatatccga ggccgtatga tctccgttgt tacggatagt aatggtatag
     1141 gtgtaaggaa caccggtaaa caatgtatct ggttcagact tatacacctg caaatcgggc
     1201 aagagtgtaa tcgaaaaaac tgccattgca ttgttattgt tgtattcgtt ttcaggtacc
     1261 ggagtaatga ttgatgcgta attacggacc agtgtaactc cgggattata agtggccgtg
     1321 gggtcaacca tcattgaata aacaatctgg ccgctgtcgc ccggattcaa agaacccaga
     1381 ttccattgaa tcatgccgcc tgaaacgatt ccaccattga gcgaaaccgg gttcagatgt
     1441 gcattatcaa aaaagtcctt caacactaca ttatctgcca cacaatttcc tgcattttta
     1501 tatttgagtg tataaaaaac atcaccacca atcacagcag gaattggcac ggtcaccaat
     1561 gttttttcta tggcaaggtc gggatgtccg aaaatgtttg taatggctgt agctgtcgga
     1621 tatcggcttt gattgagata ggaacattca accatattgg taatggttcc cgaacaaatg
     1681 ggactatcga caacacgaac tgttacctga attgtttcgg cactgtcgag accataaccc
     1741 aaggtaccaa gcgaccatac attatttgtg cctgcatcgg gagccggcac ggcgctgaca
     1801 aattcaatat tgggatcata tgtttcatac agtttaaaat cttctgccgg atatttgtcg
     1861 ttggaattaa atacaaaaat ggtataagta aattcctctc cccgttcaac agggtctttg
     1921 gaatcaactt tagaaatatt aatcagcgga tatttcggcg aacccagaat ttctgtttca
     1981 aggtaaccta catcagaggt tgttgggcaa tacaccgaag ttgctgtcac atcgtgacag
     2041 gaaaaatgaa aaggattgga accgatgggc gccgtgagtt caatcagaaa ctgatatgta
     2101 ctgaatggcg aaatccatgg ggtttgcgta atttcatttc cacccatatc cagaagtctc
     2161 gaaagcaggg ggtcaaaatc ggttccaatg caggcataat tgacaaccgt caaatcaaac
     2221 ttatcgtaga tattgccatt gttattcacc tgcatcagat aatacaaaac agtattggta
     2281 gccgatttga taaccccgct ggcatcgttg atctgcacac tgcacatctg gttgaccgtt
     2341 gtatatgtgg tatcacccaa aattggcgga tttgtgacat cgctctgaat ggaagtataa
     2401 ttgctgataa tattacttgc agccggcata taatatccgt ccgttgatcc agtaattcca
     2461 tcgcctggaa ctccggcccg aatctgaact gtaatctgac gaatgccggt accaagcaat
     2521 tccagctcag ggatattggt tttgtcccat gtaagaacat tacccaccaa tgtgcctgca
     2581 gggttggatc ctacataagt gtacatattg gcagcaggca gataatcctt gattacaaca
     2641 ttacccgcct ccactgttcc aacattggaa tattcaatga tataggtaat aagttccccg
     2701 ggagcagcag caacagggcc tgttttatga atgctcagta tttctgaatc ctgcgcagtg
     2761 gcccaactct gcaaaagaaa aaatgccagg gcagtcagaa atgacgttaa taattttatg
     2821 tgtgttttca tgatagtgct ttttaaatta gtattcagtg actttacatt tctgctatca
     2881 gtctcccctg atatgcattt tcccgtacaa ttgaaggtcg atttttttca tggctttata
     2941 ttttaagtgt tgttattctc ttattaatca cgatatatta attagcaaac attgctgcat
     3001 gctccttaaa aaaaccacac ctgcctcctg gcaaatgtgc accatacatg attccacgtg
     3061 caaatgatga acatttattg tccgaaatct cagtgttgct attaaataca aagtgcctca
     3121 tcttcaactg attaaatgac tcttcaataa aaaaacaatt atcaccccct aaagtgactt
     3181 tttaaaagat gaacgttatc taatttacga caggcaattt acaaccgcaa gggtcaacat
     3241 atcattcata catgctatat atcatatcta caaccaattt tgtcgatatt tttgcattat
     3301 ttatctacgc tgattgtttt tgaacgctat gtaaaacgca acgctgaagc gacatgtacg
     3361 tcatttcaat ttgtcgaatg tgaaaaaagt caggaaaaat caatgtgcag cttttgagaa
     3421 ttagtttaac agaagtacat taatctcgtt aacaaaatgc tttaacgcat aaatttgccg
     3481 atttatacat tttactatat ttgcatatat attcgctttt taaacacttg aataaaacgc
     3541 ggaaaccttg acaatacacg agaatctata aatcacccca tatgaaaaaa atataccttg
     3601 ttcttatttc tctgttcctt gcggcaacca catcgcaggc acaactgatg ctttcatggg
     3661 caaatacttg tggaggagca aataacgatt atgtttacag ttgtgtcaaa gacacatcag
     3721 gaaatattta cactgccggc tattttagtg gcaccgtaga ttttgattcc ggcgccggca
     3781 cttataacat ttcctcttca ggcattgatg catttgttat gaaaacagat tcctcaggca
     3841 ctttgaaatg ggttaccaaa ctgggaagta ccggtactga tcaggctatt tcactggctt
     3901 tcgacaacaa catgcatctt gttataggtg gaattttcaa cggcaccgtt gattttgacc
     3961 ccggagccgg cacagccaca cgctcgtcga acggaggaga cgacttcttt gtccttaaac
     4021 tcgacacttc cggtcaattc atctgggcaa aaactgtcgg cggcaactcc aatgactgga
     4081 ttaatgacat ttgtgttgat ccctttggaa ccatatgcat tggaggtcag ttcaaaagca
     4141 caaacagtgt tgattttgat cctggaacgg gcacagctac atcatcaacc agcggaacat
     4201 ggtcgggttt tgttttaaaa tttgatgttg acggaaatta tatatggcat aaaaaatttg
     4261 caggcagctc caatgtgatt gtctggaaag tgctttccga ttctctcaaa aacgtctata
     4321 tcgatggaac tcttgccgga acagccgact tcagccccga tacaacattg tcctcggcag
     4381 gcggaaccga catatttatt ctcaaactcg actcggcagg cggttttaca tggataaaca
     4441 aaatcagcag ccccgccaac cttgatgcac gcggatttgc cctttacaaa aaccaaagct
     4501 tttacttgac cggattcttt gaaggcaccg ttgatttcaa cccgaggggc ggtatttata
     4561 atttgacttc tgcgggaaat accgatgtct ttatcctaaa catggataca gccggcaact
     4621 ttatctgggc gaaaaaaatt ggtgccgcaa atgctgatgg cacatacgga gcatatatta
     4681 atgatgcagg acaattactg atttcgggag gatttagctc cacggtaaac ttcgatccgg
     4741 ggctgactga ttacaccatt acttctaatg gctcgggcga tgtattcgtg atgaatacag
     4801 acttaaacgg caattttaat tgggttaaat cgtttggagg cgtcaacatg gattattctc
     4861 tttgcgtact tgatctcgat tctgtgcatc cggttgtttg cggctcctat tccggaacgg
     4921 ccgattttaa cccgggaccc ggtgtgtatt caaaaacatc aaacggactc ggcgatattt
     4981 tcgtcgtcag actcgaacca tgcgttacag cagaaggcta cgacaccatt caggcctgtg
     5041 attcatatac atggattaac ggagtcacat atacctacag tatttccgga atttcacaca
     5101 cccttccagg tgcagctgcc agcggatgtg acagcattgt acatctgaat ctttcaattt
     5161 atccctctta cttttttaca gaaaaagata ctatttgtgc cggagacgaa ttgttctgga
     5221 gaggttatca gcttacaaca ggcgggcttc attacgacaa tctcacaacc attcacggat
     5281 gtgacagcgt atatgagctt cttctcaccg ttattcagga tcaggaggta atatccattg
     5341 aaaacatttg tgccggcgac agcatcttct ggcgaggaat gtattttaaa acgacaggaa
     5401 actactacga cagcgtgacg catacaagca gctgcgatac tgttttcatt ctcaatctaa
     5461 cagtaaatcc aacatatttt tcttcgcaat ctgctggaat atgcagcggc gaaagcttca
     5521 tctggcacgg caatccatat acaacagcag gtatgtatta cgacagtctg gttactcaac
     5581 atggatgtga cagtgtgttt gagctaaacc ttgttgttca tcccgtttat cttaatcaaa
     5641 tcaatgccga aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggaattt
     5761 attacgacag cctgcttacg caaaatggct gcgacagcgt gtttgaactt gatcttgttg
     5821 ttttcgattt acccaatgtc gatattataa gcactgattc ctttgtgtgt gtttacaata
     5881 caccgttaaa tcttgctgca atacccgcag gaggtgtctg gaacggacaa ggattaagcg
     5941 gaaataattt tgatcctgca gctgccggaa tcggatcctg gataattacc tatcattttg
     6001 ctgataccaa cggatgcgaa aatgctgata caatcacaat attggttgat gcttgcaccg
     6061 gactggaaaa cgaagttttt tccggaatca ctctataccc caatcccacc tccggccagc
     6121 tctccataac attccccgca gaagatgatt acacactttc attcatcgat gcactaggac
     6181 aaatcattct gacaacaaaa atctctgcac aaaaaacagt acagattgat ctgaaatcct
     6241 ttgcgcccgg tgtgtatatg ctgaaaattg aaaacgctga gggctttgtg ctgaagcggg
     6301 ttgttttaga gcggtgacca gtgagtagtg agcagtgagt ggtgatcggg taatgattat
     6361 tggaagcttc atctaaagcg gtgatcggtg accagtgagc agtgaacttt gcttatcagc
     6421 caaggagcgt tcctctctat ttaaacttca tcgttttttg ttcagtgaga acttgtcacg
     6481 cttcggcggg atgattctcc gatccatccg tacttcaatc aaaaatctaa aatcaccgat
     6541 cggtattctt atctcgttct gtccatgatt cggcattctt atgagttcat aaagttcatc
     6601 aggtttataa agtaaaaagg cacttcataa atttcagcct atccttcaaa tttccatatt
     6661 cagtgttctt gattcgatgt ttacttcctc cttcagatca aaaatcacat atctcaaatc
     6721 ttatttctta aatcttaggt gtcagccaag tccgactgtg atgctaatcc acgaggtgtt
     6781 gatgtttcct ccgggtgctc cgcgataaaa ggaattctat tttccaattt tacaccttct
     6841 ctttaatcct tacaagcacc gccggaatca gcatgaatgc cgacaccgca atcagtcccc
     6901 agccgatgct gtagcgatcg gcgacaaatc cgatgacagg agcaattgca gctgcaatca
     6961 gggtttcagc aagagactga aaagacagcg aactgcttaa aaccgtcgga tcaattttat
     7021 cagtcagata acttatgcct attggtttcc gtaaattttc aacagcataa acaatcacaa
     7081 aaagcgctgc tgctgcgata aaccagtcgt atgaatagca gattcccgca ataagtccga
     7141 acaaaaatcc ggagagcaat gtaatgttga gtggtgctgc aacatgttta aacctcttcg
     7201 caaaccggcc cgcactgcgc gatgcaaagg cggttgtcaa atgaatgcag aagaaaataa
     7261 ttccgatcag aatggccgat cgctgctttt cgtgcagcat cagaaacaga ggcatcgaca
     7321 cggcaatggt ctcaagtaaa ggttgcagat agtctttgat tgccttatag aaacccgtgt
     7381 aaaaactgag gtttccaacg gttttcaata cagttggatt tttgaaagta gtgattaaat
     7441 cgcgtatcac ctgacgaaaa ttttctttca gatcgcttcc tttgtgacga acaatttctc
     7501 catcgagttc tttcgggtaa gtggtcatca tgatcagatt caaaaaatac ggaaccgtgc
     7561 tgaaaataaa aacactctga taatcgccgg tgaaaaacac tattactgcg gcaacgagtg
     7621 atgacacagc agagccgatc tgtgaccacg agcgcgtatg tccgtagtac gcagttttct
     7681 gatccttcca tcctttcagt cggaggtaat caatgatcat acttttatgt gttccctgcc
     7741 gaaatgcatc gccgaatccg taaaaaataa acgacagcat gaacaaccag aatgcgttgg
     7801 caaaaaagaa aacagagaac gaaatgatgt aagcaataaa agcaaaaatc attactttac
     7861 ggcggccaag cacatcggca ataataccgg caggaatttc catcacatac accatcactt
     7921 cgcgaaacga gatcagaagc ccgatttcga agaacgataa gcccttgtca agaaaaaata
     7981 aaaatataaa cggatcgaaa aaccgcagat ttttcagaaa accgtagctg cagaatttga
     8041 aatattggga gtctttttga aattgtgctg tcatgatatc aatttgaaaa tttgaaagtg
     8101 tgctaatttg aaaatttgat aatgtgccaa tttaaaaagt ggaactttgt tcatgtgaaa
     8161 atgagtgaac acaagatacc gtaaaaatac aaaaacataa acatgaaata caaactactt
     8221 taatgatgtg tgataagcca tgagtgataa atcggacaca atagattttt cccgcggtgg
     8281 gcgcgtgtct ctgcatgcaa ccacgtaaaa cagcctgtca cgttttacgt gatggcaagc
     8341 tgtttcacgt tgacgcgtga ccatgctcag cacaaaaaaa gcgcgcgatc tacatgtcaa
     8401 cgcctcaaat ctttggttaa aattaggcgt atcggacgtt attggtagaa aatatcactt
     8461 tatttaacgc ataaattgat tttgttatca gaatttgtat atttgtaaaa aagggattat
     8521 gaaaggggga ataattttta ttttaatttt aatgtttgtt gcttttaata atgttcaagc
     8581 tcaaactgaa aacacaaaag ttacgcaaaa atacgaatac agtttttcag gaaaagcaac
     8641 aacgcaacaa cttgaaaagg ttgaatcaga attatacaga attgaaacaa tcactcaggt
     8701 taaaatcagc tacaagcagg attcattcaa aggcctgttg tttttcagca gaacatttta
     8761 ttccggcccg ggtgaattga ccgacgattt caatgctgcc cggataaagc agatactttc
     8821 cgaagccggt cttatacctg aagaattcaa agaattataa aaaatatctg aatcatgaaa
     8881 atgtcgttca ttttcgggat gattgtgtgt attgcttttt catttgcatc gattgaaacg
     8941 caggcacaga gtgatggttg ctcggctgca accgccatcc caattacagt caattgctcc
     9001 agtcctgttt caggaacaac ggcaggagcc acacaaacca ttgccggttg cgtcggtacc
     9061 gccgatgacg atgtgtggta caccttcacc gcaacggcca ccagtcacat tatcactgta
     9121 acaccatctt cgggaatgga cgccgtggta cagctttttt cgggcgattg tgctgctttg
     9181 atttccatga attgtatgga ccagggcttt accggcgaag cggagactat ttatgcaaca
     9241 gggctaacca ttggtgcagt gtataaaatc cggatctatc attattacac cggatatggt
     9301 tcaggcacat ttacgacatg catcactacc gctccaccag cgccttcaaa cgacaactgc
     9361 agcggcgcat ccttgctcaa tgtaaatgca acctgtgttt caactgctgg tacaacggtt
     9421 tctgcaacgc agtcgcagcc cggttgtgcg ggcactgctg atgatgatgt ctggtatagg
     9481 tttgtagcta ccaatgctgt acaaaccatt caggttacac caagcggaac cgcagatctg
     9541 gtgctggaat tattttcggg ctcttgtagt gtgctcacca gcttgtattg cgttgataat
     9601 ggtttcagtg gcgatgccga aaccatcagt gcggttggtc tgattccagg cacaacctat
     9661 tacgtcagaa catatgacta ttattcagga aacggaggaa ccaccttcga tatctgcatc
     9721 atcggaacac caactcctac gcccaccaac gacgaaccct gtgatgccat ccaattacct
     9781 ccggttactt catcatgtga ttatctgtca ttcacaacaa ccggtgcaac aacaaccacc
     9841 tctgcgccaa ctcccgcttc atgtgcgggc ggcagcagcc ctatgcaggg tggcttcaac
     9901 aatataccgc aacccaaaga tgtatggttt gctatcacag taccatcatc aggcattatc
     9961 agcatcatgg cgaaacccgg ttatggattc agcgatgcag ttatggcgct gtattccggc
    10021 acctgcacct cgcttacgca gattgcctgt tccgatgaca ataattatcc gttaacagcc
    10081 aatgatttca aaccttttct tcttgcatcg ggactcacgc ccggaacaac ggtatatctg
    10141 cgctactggg cgtttaacgg aaacacaacc ggcgattttg gcttttgcgt tacaacccca
    10201 atcaacgatg cctgttcaac cgcgctctac atctgtgatt tgaatggata caaaggaact
    10261 accggcgagg cctacacccc cgacagacca ggaaatatgc gtggtaatgc tgaaacaaac
    10321 aatcctccgc tttatactta tacagcggga acctgtcagg gaggcatttt tggcgcaggc
    10381 ggcgtttggg gaaccggcgc ccccaactgc gatgtacaaa tcaacaataa ttcatgggtc
    10441 agatttaccg cagccaatga ttctgcagtg ctgaatgtcg atgtgtatga ttgttactcg
    10501 ggaacagttg ccggcattca aatgcaggta ttctcagccg gtagtgcctg tgcagatttt
    10561 acaccggttt cgaattttga agaaggggca acacatattg aaattacggc acgcggactt
    10621 actgtcggca gcgattatta tctaatgatt gacggctttg ccggcgacat ctgcaattac
    10681 accatcagtg ccgaatcggg agtgcagttt ccaaatataa cagcggcaca ggatcctata
    10741 tgtgctggaa attccaccac gttgtttggg cctcccggtg caaccgccta tcattggtat
    10801 ccgggcaatg aaacgactca gaatatcacg gtgaatcctg caacaaccat gacgtataat
    10861 cttgaagtaa ccggcgtttg cggttatcgt caatatcttg attacacact tgatgtaaat
    10921 caattgccca ttgcaacagc atcttcgaat agtcccgaat gtgccggatc aacattgaat
    10981 ttatttggaa gtggtggcac aatctatcaa tggacagggc caaacggttt cagttcaaca
    11041 tctgcatcac cttccatcaa caatatcagc agtgctgcag ccggtacata caatcttacc
    11101 gtgaccagtg cagccggctg cagttcttcc actactacaa ccgttttagt caatcccaat
    11161 ccaacagcca atgcaggagt ttctcaaacc attcctttcg gaacaagtac aacattaagt
    11221 ggcagtgcat cgggtggcag cggcacttat ctgtattcat ggtctcctgc aggattgctg
    11281 gtttcttcaa atattgcttc acctacaaca acaaatctga caacaaccac tgttttcacc
    11341 ctcactgtta cagatcagac caccggctgc acttcgacgg ctcagacaac agtaaatgta
    11401 agtggcggtg cattaaccgt aaatgcttta gccagtgctc caggagtttg tgccggcaat
    11461 ggcattaccc ttacggcctt ggcctcgggt ggatccggct cctacaccta ttcatggtca
    11521 tcaaacccat caggattctc atcctccgac gacaatcctt atcatattcc ttcaggaacg
    11581 gcaagttata ctgttacagt cagcgatggt ttcaattccg caacatccac catcaatgtt
    11641 attgtttatc cacaaccaac agcgacagca tcgaataccg ggccttattg cgcaggagaa
    11701 aacattcagc tttctgcttc gggaggcgga acttattcgt gggttggacc caacggattc
    11761 agcagctcgc ttgccaatcc ggttattaca tcttctgctg ctgtcaacaa tggaacatat
    11821 actgtaattg tcactagtgc cgaagggtgt actgacagcg aaacaacgaa tgtaatcatc
    11881 aatgatattc ctgttgcaac agccaataac aatggaccat attgcagtgg tgaaacggtc
    11941 aatctgtcgg cctcaggagg attatcgtat gcatggagcg gtcctgcagg tttcagttct
    12001 tctgccgcca acccggccat tactgctgca actcctgctg tttcaggcac ctactccgtt
    12061 gttgtttccg gcactggcgg atgcactgca acagcatcaa catcagtact gatcaatccg
    12121 ctgccctcag tgatttgttc taatggaggt ccctattgca caggcgaaac catcatgctt
    12181 tcggcatcag gcgcttcgga ttattcctgg gccggaccag ccggattcag cagcaattct
    12241 caaactcctg aaatcacaaa cgcgcagctt tctcatactg gcacatacag cgttgttggc
    12301 actgatctga atgggtgcat gaatgctgcc accaccacgg taaacgttaa tcaaagtccc
    12361 gaaatcaaca tcagtggtga aaccgaagta tgtcaggatg gcaccgctgt tctttctgca
    12421 aacggagcaa acctatatat atggaatacc ggcgcaacaa cagcatccat caccatcggg
    12481 ggaattgctt cagccacata tgtggttacc ggcactaccg gcacttgttc cgacattgac
    12541 agtatttctg tcacggtaaa tccaaatccc acagccaatg cgggagccga cacaacaatc
    12601 gaagagggac aaagcattca gctcaacgga agtggaggcg gaacctattc atggtctcct
    12661 cccgatggtc tttcagaccc tgacattgca aacccgatat gcaatgcaga agacacaact
    12721 gtttacatac tgactgttac caatgaattc ggctgtacag ataccgacac cgtgattgta
    12781 agagttgacg gagattgcgg aagcatctac gttccaaacg cattcagtcc gaacggtgat
    12841 ggaaaaaatg acgtctttgg cgttatgaac cgttgtcttg aaacattaaa tctgaaagta
    12901 tataatcagt ggggtaatct ggtattcgaa acaaacgacc ccaatggcag atgggatggc
    12961 acttttagcg ggacaatgtc ggaaagcggc atttacagct attggttcga tggatcacta
    13021 agcgatggca ctcttgtgaa tggtcaggga aattttgtat taatcagata aacaaagcaa
    13081 catgaaaaca aaaaaaacaa tggcatttat tatgttgctt ctgtctgctt cagccatcag
    13141 caacagggtt tctgcacaag atccgcactt tagtttcagc aatgcattta ttgaatatca
    13201 atcaccggga cttatcggaa cacaggataa tcaaatgagt tttcagatga tttaccgtaa
    13261 tcagtggaga aatgttggca ctgcatttca aacatatggt ctgtcgggaa attcacgtgt
    13321 ttcaaaatca acaaacactt ctttttctgt aggcggattc attttcaatc aatcggcctt
    13381 cagctatagc agtctgcttg cacatgttgg tgctgctgcc aatgtaaagc tgtctgaagg
    13441 caaaatatta agtgttggca tcggaagcgg aacacgctat gagtcactta atcctgacga
    13501 tttgatgtgg accgaccagt ttaatggcta cgaatatgat cccaatattc agcacggaga
    13561 atatttcgac agacaacaaa aagccagctt tgatttatcg gctggcatat cgtataaatt
    13621 tatgtccgaa gcgcccaaca tcgatcgttt ttcaaaaagc agcggaacca tttctgccgg
    13681 attctggcac ctgaaccagg gaaaacgact tacgctaagc gacgaacaag ccgacatcaa
    13741 aatggttatg atgaccgatt tgacatttgc catgggagaa gataaaaaag cagcattcaa
    13801 tccctggata ctgggaatgc tacagggctc ggcctatgaa tttactgtcg gaagcgacat
    13861 taaattcttt ctgaaacaaa gcgataaata ttctgtttcc cgtgctatct tcagcattgg
    13921 aggcgcttac agactgaatg atgccgcaat ggcgcgtatt atgtttgatt atgcaaacta
    13981 tggaatcggt attgcatacg aaatcaacac atcctcgctg aaagaggcca gcaaagggtt
    14041 tggatgcttt gaaatattca taaaatacag tccgatgtcg aagagctttt cattgtattg
    14101 atggatgaag ttatgaatga tgagttatga gttggatgtg aaaatgtggc gcggagttta
    14161 tcccgctgga agcggggcgc atgtgcgact tcgtcgatgt gagaatatgt gatactgcaa
    14221 atgttgtcga aaatgtcaag ccgaaaa
//
LOCUS       DAOPKE010000071        13755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4679_length_13755_cov_12.864599, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000071 DAOPKE010000000
VERSION     DAOPKE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4679_length_13755_cov_12.864599"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(479..1426)
                     /locus_tag="PKY63_09270"
     CDS             complement(479..1426)
                     /locus_tag="PKY63_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF00843.1"
                     /translation="MNSRGLILFLDTTHPLLPQRLEEVGFEVKLFSGTLDELKTMLPD
                     VTGLVVRSKFTLSRDVLECAPNLRFIARAGAGMENIDVAYAESRGISCLSSPEGNRDA
                     VGEQALGMLLMLMNNLRRADNEVRNSIWKREANRGFELTGKTVGIIGYGNMGSAFAQK
                     LSGFGCRLIAYDKYKTGYGSAIVEEQSMQTLFEECDVVSLHVPLTEETTYLANENFFD
                     SFRKPVWFINTSRGMVCKTSALITALEAGLVLGAALDVHEWEDFSFENFFKQELPPEF
                     TWLTQSDKVVLSPHIAGWTHESNVKHAEVLFSKIVALRL"
     gene            1885..5205
                     /gene="secA"
                     /locus_tag="PKY63_09275"
     CDS             1885..5205
                     /gene="secA"
                     /locus_tag="PKY63_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPF00844.1"
                     /translation="MATIWQKLFGNKADKDMKEVTPYVEKVHVEYEKIKKLSNDELRA
                     KTLEFRAALIAAVKDEEDQIEALKNRIETEFEMPIEEKQKIYSEIEKLEKKVYEITQE
                     KLTEILPAAFAVVKDTARRFKENEEIEVTATEFDKELAAKRNSIIIKGDKAYWKNAWT
                     AGGTTIAWDMVHYNVQLIGGYVLHSGKISEMATGEGKTLVATLPMYLNALPGKGVHVV
                     TVNDYLAKRDSEWMGVLFEFHGLVVDCIDRHEPNSAARKKAYLADITYGTNNEFGFDY
                     LRDNMARGIDEIVQRGHNYAIVDEVDSVLIDDARTPLIISGPTSKGDHQEFDELKPFV
                     VTITNAQKTLINGFLAEAKRLLSKENPSSEDEKLGGVALLKCFRGLPKNKALIKFLSE
                     PGMKTLLHKTENLYLSENMKNMHLIDDELFFVIDERQNSIELTDKGIDLLTEKTGDAK
                     FFILPDIGSELADLEKSELSDTERIEKKQLLRQDYAVKSDRIHTMNQLLKAYTLFEID
                     VEYVIIDNKIKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYF
                     RMYKKLAGMTGTAETEAGELWNIYKLDVVVIPTNRPIVRKDHHDLVYKTKREKFNAVI
                     DEIVNLKEAGRPVLVGTTSVETSELLSRMLTMKKIKHSVLNAKLHAKEADIVAMAGQS
                     GSVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSS
                     QFYVSLEDDLMRLFGSDRIAGIMDRLGLKEGEMIQHSMISKAIERAQKKVEENNFGIR
                     KRLLEYDDVMNSQREVIYNRRHNALYGDKLSIDLLNMFSDIAETIVAEGQDSRDFDQF
                     TFEVMKTFAVEPGIDEKEFFSENTSKMAEKLFTHSYGEYQQKCDMTAQRTFPIIKEIF
                     ENANRKFDDVAIPYTDGKKSFTALANIKKSYESQGKEVMRGVERSLALAIIDDAWKEH
                     LREMDDLKTSVQNAVYEQKDPLLIYKFESFELFKQMLTKISKDLLSFLTHGHVPLAED
                     RKLSEAEQARRSNNPNLKTSRESNIPGTQHPKYHDPSANNTTKEPVRVEKKVGRNDPC
                     PCGSGKKYKNCHGKDLSD"
     gene            5274..6077
                     /locus_tag="PKY63_09280"
     CDS             5274..6077
                     /locus_tag="PKY63_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HPF00845.1"
                     /translation="MTKRKKTRINRWYIVLSVLILLVFLLIFTNPGRYFISWLRYQSG
                     LQINPPEKITQIGVPLPQGFEVFGIDVSRYQNNIDWLRVADFKSGRFTVEFAFIKATE
                     GKTLRDPSFSDNWKGAQDAGIPRGAYHYFKPGVDAEDQANHFCRTVKLEKGDLPPVLD
                     IEETARGMSRNALVKSLWTWLQIVERHYGVKPIVYTGAVFYETYLSGTSIEKYPLWVA
                     HYYQNRPATFAKWEFWQFSDKANIDGISGSVDVNVFFGNNDQLIQLTRQ"
     gene            6213..8687
                     /locus_tag="PKY63_09285"
     CDS             6213..8687
                     /locus_tag="PKY63_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF00846.1"
                     /translation="MKYLIVGGVAGGATTAARLRRVDEKSEIIVFERGEYISYANCGL
                     PYYIGGTIAERENLFVQTPATFGQRFNLEVRNFSEVITIDREKKAVTVKKVNSGETYT
                     ENYDKLVLSPGAEPVKPPIPGIQSEGIFTLRNVPDTDKIKEYIETRKPRHAVVVGAGF
                     IGLEMAENLHKAGLRVTIVEMAEQVMSPLDYSMAAMVHQHLKTKNVEFYLKSSVASFA
                     QKNNRIEVKMNSSKTIETDMVILSIGVRPDTQLAKTAGLTIGASGGFVVNEFLQTNDE
                     DIYAVGDAIEFANPITKTPMITYLAGPANKQGRIAADNIVFGNKKTYKGSVNTAIAKV
                     FDITVAATGVSGKQLGKLNIPHIDSTTHSSSHAGYYPGALPMTIKISFAPDNGRLLGA
                     QIVGYEGVDKRIDLLSTAIQQGATVYDLAEIEHAYAPPFSSAKDPVNIAGMVAENILN
                     GVSKQISWREVHNESRNNIFLLDIRTNDEFQLGSIDGAVNIPLDELRDHLNEIPKDKK
                     IIAFCGVGLRAHVACRILTQSGFENVFNLSGGLKTYETASQKQSNEDIFANDYIGIDD
                     LIYQGSKEKPVAKSVAAEKIKIVDACGLQCPGPILKLKKSMDEIPEGEAIRITASDAG
                     FYKDVAAWAKVTGNEILELSQNGGEITAVVSKGKPIAPSLQTNATGTSATLVVFSDDL
                     DKALATFVIANGAAAMGKKVTLFFTFWGLNVIKRLDKPALKKDFFGKMFGMLMPKSSR
                     RLALSKFNMAGIGRSMMRKRMANKQVDALENMILTAQENGVEFVACQMSMDVMGVSAS
                     ELMDGVQIGGVATYLEKTEGNLNLFV"
     gene            complement(8704..10746)
                     /locus_tag="PKY63_09290"
     CDS             complement(8704..10746)
                     /locus_tag="PKY63_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00847.1"
                     /translation="MKIIPIIFLCVFALTAQATVYTVTNTNTSGAGSLHAAITTANTA
                     PGAPHSIVFNIPVSDPNYNAAQGVWIIHITNAARLPYILGSNISIDASTQTSNVGNTN
                     PDGPEIVLDGGGSTDFCFFAMNAANITIRGFNIRQFTYGIQVSGSTSTGWVIAGNYIG
                     TNEDASDTAGCYIGVELLNFAGYATIGGLNAADRNIISGNEHIGLRLLSSSHNTVINN
                     YIGIDRTGTFALRNYDGLSLEAFTQYNQIGGNSPAARNVISGNVAYGLPLIGYDTQHN
                     IIQGNYIGTNASGTAAVENTYGILFDDGSHYNTVGGLGSGEGNLLSGNSGYGLFLYNN
                     GTTENWCYGNLIGTDFLGTSAVPNGNGVVIDGIATRHVLDKNVISGNIQNGIAIHITG
                     TNEHHITRNFIGTDITGTLPLGNGADGIRIAEGGQYNIIGGPDSGNVIAYNGGNGITV
                     LTAADYGNTFSENLIYGNAQLGIDLYPQGVTVNDAGDSDAGANQLMNFPVLDSASFDV
                     GLGKTTLYGHLDHPLAQYCRVEFFGSSGDASGHGQANEFLGNVSPAAGGTFSFEITTV
                     LAWNYVTATATDSSGNTSEFALNLELPGPNAISEQENENIFIVYPNPASDFIFVENSA
                     GGDFQLQLINSAGQIVLSQNYFSHEAIVDVASLERGAYLVVISYGAESDVFRIQLH"
     gene            complement(10869..13643)
                     /locus_tag="PKY63_09295"
     CDS             complement(10869..13643)
                     /locus_tag="PKY63_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPF00848.1"
                     /translation="MRQFVCGVFFLLCCFGTRAANYTVINTNDNGAGSLREAITSANA
                     SVGADNIYFGIPTTDPNFDALSGTFTITLLSSLPMITTGYINIDATSQQTNQGNTNVH
                     GYEIVLAKGAGTFDYPLMLVSPGNTIKGFIIQGFNYGIMIYNSTATGNIITQNYIGTN
                     HLGTAPAAIPNTYGVGIANGASANIISNNVVSGNTIAGIAITGANNNTIKNNRIGTDS
                     SGTFAVSNNFGIAIQNASSNIIGSLVSGEFNLISGNTTAGIVIDGLPSSGNQIFGNRI
                     GTDVYGTDSLPNESGIILSYASSTTIGGSTFSHRNIISGNRGAAIVMNGTGTRNNVVK
                     GNFIGPDITGVAPLHNYSGINILSSANSNIIGGTLPAERNIISGNLEMGVYIEAADSN
                     VLVNNFFGPDSTGLDAFRIGGDTLLQSNGVEFNTVSKHNRLGGYTPAERNVVSGNRVY
                     GMVYYGNCSYNPVIGNYIGVDASGNNALPNATGICVDGGSNHNPIINNVLSGNYSYGI
                     FIVTTGTFYNTMTGNILGLNAAGTDTVPNDAGLLIGGGTKFNIIGGTTPSERNIFSGN
                     RFGGIEIADQGTSFNTIKGNYVGTDITGLQARPNLYGISIISECSQNTIDSNIISGNA
                     HYGLLFFENADSNVVTRNYIGPGADSTTALGNGTAGVVLWNGSSYNQIGGGNLGNVIA
                     YHDSCGIAVKDNNTLYNTLSENSIYDNSLMGIDIFPEGPNANDVDDVDSGPNEMMNYP
                     IIWTPEYIPWAQQLTIAGSFQMTGMADGIVVEVFEAAPDSLINCGEAKRFIGRTLCST
                     MGEWLITVPWTEAGTVITATATSISGSTSEFAPNVTVITTLDELIKNTVSVYPNPASD
                     YILFSAQGAGLKSVSVFTADGRKVFEQNEIDAEKFEWIFKNELQSGVYVVRVLTADCR
                     ILVSKFDVVR"
BASE COUNT         4034 a         3099 c         3017 g         3605 t
ORIGIN      
        1 cgtcttgtcg cctcgaatat ggcgcacctc agactttttc agcaggccct aaatacacaa
       61 tgctctgaaa gaatctctct gcgtctttgg tccgccttcg tcggactaaa ctccctctgc
      121 gagccattcc tgagcgaccc actgaatcag tcttcatcgc atgcctgcct gttgcagaca
      181 gatgaagggt gattctattc gcggaagaaa gggtgcaagg gcttcacgtg aaatcacgta
      241 aaacgtgaca agctgtgaca agtcgagggt tttccaagag atcccggcac agtcagcaaa
      301 gctgtatctc aatcagcgac cgggaactca gtagtgaaaa tattttcgtg tctttgtgtt
      361 atagtggcaa atccctctgc gtctccacga cctctgcgag aaacaattga atgctctctg
      421 cgttctctgc gtgaaacaat tgaatgctct ctgcgtctcc gcgtcctctg cgtgacactt
      481 ataacctcag cgcgacgatt ttggagaaca gcacctcagc gtgcttcaca ttcgattcgt
      541 gcgtccagcc tgcaatatgc gggctcagca cgactttgtc gctttgcgtc agccaggtga
      601 attccggtgg cagctcctgt ttgaagaaat tttcaaaact gaaatcttcc cattcgtgca
      661 catcgagtgc ggcgcccaat accaaacctg cttctagtgc ggttatcaga gctgatgtct
      721 tgcacaccat accacgcgag gtgttgatga accagaccgg tttacgaaaa gaatcaaaga
      781 aattttcatt tgccagatat gttgtttcct cggtcaacgg cacgtgcaga ctcaccacat
      841 cgcattcctc aaacaaagtc tgcatggatt gttcttctac aatcgcgctt ccatagccgg
      901 ttttgtattt atcataggca atcaagcgac aaccgaaacc gcttaatttc tgcgcgaaag
      961 cgctccccat atttccatat ccgataatac caacggtttt cccggtcagc tcaaagccgc
     1021 ggttggcctc acgtttccag atgctgttgc ggacttcgtt gtcggcgcgc cgcaggttgt
     1081 tcatcaacat aagcaacatg ccgagtgcct gttcgccaac agcatcacga ttgccttccg
     1141 gactgctgag acatgaaatg cctctgcttt cagcatatgc aacgtcaata ttttccattc
     1201 cggcaccggc acgtgcaatg aaacgcaggt tgggtgcgca ctccagaaca tcgcgcgata
     1261 aagtgaactt actccgcacc acaagcccgg tcacatcagg caacattgtc ttcagctcat
     1321 caagcgttcc ggagaaaagc ttcacctcaa atccgacctc ttccagtctt tgcggaagca
     1381 gcggatgagt agtgtcgaga aataatatca ggccgcggga attcatgatg caaagataca
     1441 aaatagtggt caggttgaat ggttaatggt tattggtcaa tggttaattg gtcgaggtgc
     1501 cgaaggcgtg aaagaagctg cgcagcacct taattattcg catccgcctc cgtcggatta
     1561 aactccaggc gataaatatt aatagcccgt atgcctcacg tgaaacgtga caagcttcgg
     1621 gctccgggaa cgagcaagta ggtgatgggt tatgtgagaa ttgatttttg gccactggca
     1681 attgatatgg tgaatttgcg ataatcaaag gctcatacaa aagtcgcatc gccgaactct
     1741 ctgcgtcgat gaaatcgacc tgagtgcatc ggcttatccg cagctcgccc ccaacgctga
     1801 gaggtcgctc cgcgacgcac tcaggttggg gagaaagaaa attcaaaaaa ttcaattact
     1861 tttgtaggaa taatttcaat caatatggca acaatctggc aaaagctatt tggtaacaag
     1921 gctgacaagg atatgaaaga agtgactccc tatgtagaaa aagtacatgt ggagtatgaa
     1981 aaaataaaga aactttccaa cgacgaactc cgcgcaaaga cgctcgaatt ccgtgcagca
     2041 ctcattgcag ctgttaaaga cgaagaagat cagattgaag ctctcaaaaa ccgcattgag
     2101 actgaatttg aaatgccaat tgaggaaaag caaaaaatct attctgaaat tgaaaaactt
     2161 gaaaaaaagg tttacgaaat cacgcaggaa aaacttaccg aaatactgcc cgctgcattt
     2221 gccgttgtaa aagatacagc ccgacgtttt aaggaaaatg aagaaattga agtaactgca
     2281 accgaatttg acaaagaact ggctgccaag cgcaacagta tcattatcaa aggcgacaaa
     2341 gcatattgga aaaacgcatg gacagccgga ggcactacaa ttgcatggga catggtacat
     2401 tacaatgttc agctcatcgg cggttatgtg ctgcattcag gaaaaatctc tgaaatggct
     2461 acaggagaag gtaaaacgct ggttgcgacc ttgccaatgt atctgaatgc ccttccggga
     2521 aaaggagtgc atgttgtaac cgtgaatgat tatctcgcca aacgagactc tgaatggatg
     2581 ggtgttttat ttgaatttca cggactggtt gtagattgta tcgatagaca tgaacccaac
     2641 agcgcagctc gcaaaaaagc atatctggcc gatattacct atggaaccaa caatgaattc
     2701 ggcttcgatt atctgcgcga taacatggct cgtggcattg atgaaatcgt acagcgcgga
     2761 cacaactacg ccatcgtcga tgaggttgac tccgttttga tcgacgatgc acgaacaccg
     2821 ctgattattt ccggcccgac cagtaaaggc gaccatcagg agtttgatga actcaagcca
     2881 tttgttgtta ctataacaaa tgcacaaaaa acgcttatca atggttttct cgctgaagca
     2941 aaacgattat tatccaaaga aaatcccagc tctgaagatg aaaaactagg aggagttgct
     3001 ttgctgaaat gctttagagg tcttccgaaa aataaagcat taatcaaatt tctcagtgaa
     3061 ccaggaatga aaaccctgct tcacaaaacc gagaatctct atttgagcga aaacatgaag
     3121 aatatgcatc ttatcgatga tgaattattc tttgtgattg atgaaagaca aaattccatt
     3181 gagcttaccg ataaaggaat tgacttgctg actgagaaaa caggcgatgc taaatttttc
     3241 atattacctg atatcggttc agaattagcc gatcttgaaa aatctgaatt atccgacact
     3301 gaacgcatcg aaaaaaagca gttgcttcgt caggattatg ctgtcaagtc agaccggatt
     3361 cacacaatga atcagttgtt gaaagcttat acactatttg aaattgatgt tgaatacgtc
     3421 atcattgata acaaaatcaa aattgttgat gagcaaaccg gccgcatcat ggaagggcgc
     3481 cgctacagcg acggactgca tcaggccatc gaagccaagg aaaatgtgaa agttgaagcc
     3541 gccacacaaa cctatgccac cattacactg cagaattatt tccgcatgta taaaaaactg
     3601 gctggtatga ccggtacggc tgaaaccgaa gcaggtgagc tgtggaatat ttataaactg
     3661 gatgttgttg ttattccgac aaaccggcct attgtgcgta aagaccacca tgatctggtt
     3721 tataaaacca aacgtgaaaa attcaatgcg gtcattgacg aaattgtcaa tctgaaagaa
     3781 gcgggacgtc cggttcttgt tgggacaacc tcagttgaaa cctcagaact gctcagtcgc
     3841 atgctgacca tgaaaaaaat aaaacacagc gttctcaatg caaaattgca tgcaaaagaa
     3901 gctgacattg tagcaatggc aggccaatcg ggcagtgtta ccattgccac aaacatggct
     3961 ggtcgtggaa cagatatcaa actcgggcct ggtgtaaaag aagcaggtgg tcttgccatc
     4021 attggcacag aacgccacga atcgcgtcgt gttgaccggc agctccgggg acgtgccggc
     4081 cgtcagggag atcctgggtc ctcgcaattt tatgtttcac ttgaagatga cctgatgcgg
     4141 cttttcggct ccgaccgcat tgctggtatc atggatcgtc ttggactgaa agaaggcgaa
     4201 atgattcagc atagcatgat ttccaaagct attgaacgcg cccagaaaaa agtggaagaa
     4261 aacaacttcg gcatccgaaa acgacttctg gaatatgatg atgtaatgaa ctcgcaacgc
     4321 gaagtcattt ataaccgtcg tcacaacgca ttgtacggag ataaactatc cattgatctg
     4381 ttgaatatgt tcagcgatat tgctgaaaca attgttgctg aaggtcagga ttcacgcgac
     4441 tttgatcagt tcacgtttga agtgatgaaa acatttgcag tagaacctgg tattgatgaa
     4501 aaagaattct tctcggaaaa cacatcgaaa atggctgaga agctttttac acattcctac
     4561 ggcgaatatc agcaaaaatg cgatatgact gcccaacgca ctttccccat tatcaaggaa
     4621 attttcgaaa atgccaaccg caaattcgac gatgtggcca tcccctatac tgatgggaaa
     4681 aaatcgttca ctgcgctggc caatatcaaa aaatcctacg aaagtcaggg caaagaggtg
     4741 atgcgtgggg ttgaaagaag tctggctctg gccatcattg atgacgcatg gaaagaacat
     4801 ctgcgagaaa tggatgatct taaaacaagt gttcagaatg cagtgtatga acaaaaagat
     4861 ccgctgctga tttacaaatt tgaatcattt gaattgttca aacaaatgct tacaaaaatc
     4921 agcaaagacc ttctgagttt tcttacccat ggccatgttc cgctggctga agacagaaaa
     4981 ttatccgaag ccgaacaagc tcgtcgcagc aataatccaa atctgaaaac cagccgggaa
     5041 tcaaacatcc cgggaacgca acaccccaaa taccatgatc caagtgcaaa caacactaca
     5101 aaagagcctg tccgcgtaga gaaaaaagta ggtcgaaacg atccatgccc ttgcggaagc
     5161 ggaaaaaaat ataaaaactg ccacggtaaa gatctatctg attaagtctt ttatcttttc
     5221 gaaaaatctg atcatcttct tcggaataca agaaattcag taatagttgc ttaatgacaa
     5281 aacgtaaaaa aacccgcatc aaccgatggt atatcgtttt gtcagtattg atattactgg
     5341 tttttcttct gatttttaca aaccctggaa gatacttcat ttcctggctg cgctaccaaa
     5401 gcgggctgca aataaatccc ccggaaaaaa taactcaaat cggcgtacct ctgcctcaag
     5461 gttttgaagt gtttggtatc gacgtttcgc gctaccagaa caatattgac tggcttcgtg
     5521 ttgctgattt caaatccgga cgatttaccg ttgaatttgc tttcatcaag gcaacagaag
     5581 gaaaaacact ccgtgatccg tcattttctg ataactggaa aggcgctcag gatgcaggaa
     5641 ttccgcgagg tgcttatcat tatttcaagc caggtgtcga tgccgaagat caagccaatc
     5701 atttttgcag aacggtaaaa ttggaaaaag gcgacctgcc tccagttctt gacattgaag
     5761 aaactgcacg cggtatgagt cggaatgcat tggtaaagtc actctggaca tggcttcaga
     5821 ttgttgaaag acattatggt gttaaaccaa tcgtttatac aggtgcggtg ttttacgaaa
     5881 catatctgag tggtaccagt attgagaaat atccactttg ggtagctcat tactatcaaa
     5941 acagaccggc aacatttgca aaatgggaat tctggcaatt cagcgacaaa gccaatatag
     6001 acggcatatc tgggtctgtt gatgtaaatg tcttttttgg caacaacgat caattgattc
     6061 aattgaccag acaatagaaa taatctgctt tcgcctgcct ttattcaagc atgttgcaag
     6121 tcatgataaa catcatagct gcaacggaaa tctgcattca gatttgcagt aattttgcat
     6181 tcagaaaatc ttacgcatta aaacacattg taatgaaata tttaattgtc ggcggagtgg
     6241 ctggtggcgc aacaactgca gcaagattgc gcagagttga tgaaaaaagt gagatcatcg
     6301 tttttgaacg cggtgaatat atttcgtacg ccaattgcgg attaccttat tatataggtg
     6361 gaacaatcgc tgagcgcgaa aatctttttg tgcagacgcc ggccaccttt ggtcagcgtt
     6421 ttaatctcga agttcgcaat ttttccgaag ttataaccat cgatcgcgaa aaaaaagcag
     6481 tgactgtaaa aaaagtcaac agcggcgaaa catatactga aaactatgac aaactggtgt
     6541 tatcacctgg ggcagaacct gtgaaaccgc ccattccggg cattcagtct gaaggcattt
     6601 ttacattgcg caatgtgccc gatactgata aaatcaaaga atacattgaa accagaaaac
     6661 ctcgtcatgc agtggttgtc ggtgccggat tcatcggact ggaaatggct gaaaaccttc
     6721 acaaagcagg acttcgtgta accattgttg aaatggccga acaggttatg tcgccgcttg
     6781 attactcaat ggccgctatg gtgcatcaac atctgaaaac aaaaaatgtt gagttttatc
     6841 tgaaatcttc ggttgcttct ttcgcccaga aaaacaaccg catcgaagta aaaatgaatt
     6901 catcaaaaac catcgaaacc gatatggtca ttttatccat aggtgttcga ccggatacgc
     6961 agctggccaa gacagcaggt ttaacaattg gggcttcggg tggctttgtt gtgaatgaat
     7021 ttttgcaaac aaatgatgaa gatatttatg cggtgggaga tgctattgaa tttgccaacc
     7081 cgattacaaa aactccaatg attacctatc tggcagggcc ggccaacaag caaggaagga
     7141 ttgccgctga taatattgtt tttggaaata aaaaaacata caagggatct gttaatactg
     7201 ctattgcaaa agtatttgac attaccgttg cagcaacagg tgtctctgga aaacagcttg
     7261 ggaagctaaa tataccacat atagattcca ctacgcattc atcatcacat gccggatatt
     7321 atcccggagc attgcccatg actataaaaa tttcctttgc ccccgataat ggtcgtctgc
     7381 ttggcgctca gattgtgggt tacgaaggag ttgacaaacg gattgatttg ctctcaacag
     7441 ccattcaaca gggagcaacc gtttatgatc tggcagaaat tgaacatgcc tatgccccgc
     7501 ccttttcttc ggcaaaagat ccggtaaaca tagccggcat ggtagccgaa aatattctga
     7561 acggagtatc aaaacagatt tcgtggcgcg aagttcacaa tgaatcgcgc aataatattt
     7621 tcctgctcga tatcagaaca aacgacgaat ttcaactggg gagtattgat ggtgcagtga
     7681 atattccact tgatgaattg cgtgatcatc tgaatgaaat tccaaaagac aaaaaaataa
     7741 ttgccttctg cggtgttgga ttgcgtgcac atgtagcatg ccggatcctg acacagagcg
     7801 gctttgagaa tgtgttcaac ctttccggag gtctgaaaac gtatgaaaca gccagtcaga
     7861 aacaaagtaa cgaagatatt tttgccaacg attatatcgg cattgatgac ttgatttatc
     7921 aggggagtaa agaaaaacct gttgcaaaaa gtgttgctgc agaaaaaatt aaaatcgttg
     7981 atgcctgtgg attgcagtgt cctggcccga ttctgaaact taaaaaatcg atggacgaaa
     8041 ttccggaagg cgaagccatt cggattaccg cttccgatgc gggtttctac aaagatgtag
     8101 ccgcatgggc caaagtgacc ggaaacgaaa ttcttgaact ttcccaaaac ggcggcgaaa
     8161 taacagcagt tgtctcgaaa ggcaaaccaa ttgcaccatc gctccagacc aacgctacag
     8221 gcaccagcgc cacactcgtt gttttcagcg acgatcttga caaagcactt gcaacttttg
     8281 tcattgccaa cggcgctgca gccatgggca aaaaagttac cttgttcttt acattctggg
     8341 gtctgaatgt tattaaacga ttggataaac cagcacttaa aaaggatttc tttggaaaaa
     8401 tgtttgggat gcttatgcca aaatcgagtc gcagactggc attgtcgaag ttcaacatgg
     8461 ccggcatcgg acgttcgatg atgcgcaaac gcatggccaa taaacaggtc gatgcacttg
     8521 aaaacatgat tctgactgct caggagaatg gtgttgaatt tgtcgcctgc cagatgtcga
     8581 tggatgtgat gggcgtttcc gcgtccgagt tgatggatgg cgtacagatt ggcggcgtgg
     8641 ctacctatct cgaaaaaacg gaaggcaatc tgaatctgtt tgtgtaaaga tcattgtccg
     8701 aaatcaatgc agctgaattc tgaaaacatc ggattcggct ccataactga ttacgacgag
     8761 atatgctcca cgctccaacg atgcaacatc aacaattgct tcgtgtgaaa aataattctg
     8821 gcttaaaaca atctgtcctg ctgagttgat cagctgtagc tgaaagtcgc caccagcgct
     8881 attttcaaca aatataaaat cgctggccgg gttcggataa acaataaata tattttcgtt
     8941 ttcctgctcc gaaatggcgt ttggtcccgg caattcaaga ttcaacgcga attccgaagt
     9001 gttgccgctg ctgtcggtag cggtggcggt cacatagttc cacgcaagta cggttgttat
     9061 ttcaaagctg aaagtgccac cagctgcggg agaaacattt ccgaggaatt cattggcttg
     9121 tccgtgaccg cttgcatcgc cgctgctgcc gaaaaattca acgcgacaat attgcgccag
     9181 cggatgatcg agatgcccgt acagtgttgt tttaccaaga cctacatcaa atgaggcgga
     9241 atcaagcact gggaaattca tcagctggtt ggcgccggca tcactgtcac cggcatcgtt
     9301 aacagtcaca ccctgcggat aaagatcaat gccaagctga gcatttccat aaataagatt
     9361 ttcggaaaaa gtattaccgt aatcagccgc agtcagcaca gtaattccat tcccgccatt
     9421 ataagcaatc acatttcctg aatcaggacc gccaataata ttgtattgtc cgccttcagc
     9481 aatgcggatc ccatcagcac cattgcccaa cggtagtgtt ccggtaatat cggttccaat
     9541 aaaattccgt gttatatgat gttcattggt gccagtaata tgaatggcaa tgccattctg
     9601 aatatttcct gatatcacat ttttgtcgag tacatgccgt gttgcaatgc catcaatcac
     9661 aacgccgttt ccgttcggca cagccgatgt acccaaaaaa tcagtaccaa tcaggtttcc
     9721 ataacaccag ttctcggtag tgccattgtt atataaaaat aatccataac ctgaatttcc
     9781 tgaaagcagg ttcccttctc cacttccgag acctccgacg gtattgtaat gcgaaccgtc
     9841 gtcgaacaaa attccgtagg tgttttcaac agccgcagtg ccagacgcgt tggtgccaat
     9901 ataattaccc tgaataatat tatgctgcgt atcatagcca atcaacggaa gtccgtaggc
     9961 aacattgccc gaaatcacat tgcgcgctgc aggcgagttt ccgccaatct gattgtattg
    10021 cgtaaatgct tcaagactca atccatcgta gtttcgcaat gcaaaagttc ctgtgcgatc
    10081 gatgccaata taattattga tcaccgtgtt gtggctcgaa ctcagcagcc gtagcccgat
    10141 gtgctcgttg ccactgatga tgttgcggtc tgcggcattt aatcctccaa tagtagcata
    10201 tcctgcaaaa ttcaataatt ctacaccaat gtaacaacca gcagtatcag aggcatcttc
    10261 gttggttccg atgtaattac ctgcaatcac ccaacccgtc gaggtgctgc ctgaaacctg
    10321 aataccataa gtaaattgcc tgatattgaa tccacggatg gttatgttgg ccgcattcat
    10381 agcgaaaaaa cagaagtcgg tactgccgcc tccatcgagc acaatttcag gtccatcagg
    10441 atttgtattg ccaacattgc tggtctgcgt cgatgcatca atgctgatgt ttgagcctag
    10501 gatgtaaggc agtcttgcag cattggtaat gtgaatgatc cagacacctt gtgccgcatt
    10561 gtaattcgga tcgctcaccg gaatattgaa cactatgctg tgaggcgcac cgggagctgt
    10621 gttcgcagtt gttattgcag catgcaaact acctgccccc gaagtgttgg tgttggtcac
    10681 cgtatatact gtggcttgtg cagtgagtgc gaaaacgcac agaaaaatga ttggaattat
    10741 tttcatttta ttctcttggt tattattccc ggccctgagc cgggatctct tctatttact
    10801 tctgagctga tggtttaatt tactatttct gttgcaagag acccctggtc aagcccgggg
    10861 actcatatct atctgacaac atcaaacttc gaaaccaata ttctgcaatc agctgtcaac
    10921 acacggacca catacactcc gctctgcaac tcgtttttaa atatccattc aaacttttcc
    10981 gcatcaattt cgttttgctc aaaaactttt ctgccatcgg ctgtaaaaac agaaaccgat
    11041 tttaacccag cgccctgagc agaaaaaaga atataatcac tggcgggatt tggataaaca
    11101 gacactgtat tttttatcaa ttcatccaac gttgttatca ctgtaacatt gggcgcaaac
    11161 tccgatgtac tgccactgat gctggttgct gttgcagtaa taacagttcc ggcttcagtc
    11221 catggaaccg tgattagcca ctcgcccatg gtgctgcaca gtgttctgcc aataaaacgt
    11281 tttgcttcac cacaattaat cagggaatcc ggagctgctt caaaaacctc aacaacaata
    11341 ccgtccgcca tacctgtcat ctgaaagctg ccggcaatgg tcaattgctg cgcccacgga
    11401 atatattccg gagtccaaat aatcggataa ttcatcattt cgttgggtcc tgaatcaaca
    11461 tcatcaacat cattcgcatt tggaccttcc ggaaaaatat cgatgcccat gagcgaatta
    11521 tcgtatatgc tattttcgga aagagtgttg tataaagtgt tgttgtcctt cacagcaata
    11581 ccgcatgaat cgtggtatgc aatcacattt cccaaattcc cgccaccgat ctgattgtac
    11641 gaagatccgt tccacagcac cacgccggct gttccgtttc caagggctgt ggtactgtcg
    11701 gcaccgggac caatgtaatt gcgcgttaca acattgctgt cggcattttc gaaaaacaac
    11761 aatccgtaat gtgcattacc ggaaataata ttcgagtcaa tggtgttctg cgaacattcc
    11821 gaaataatac tgatgccata caagttaggt ctggcctgca atcccgtgat atccgttccg
    11881 acataatttc ctttgattgt gttgaaactc gtgccctgat cggcaatttc aattccgcca
    11941 aaacggttgc ctgaaaaaat attccgttca gaaggcgtag tgcccccaat gatattaaac
    12001 tttgttccac cgccaattag caatccggca tcattaggca ctgtgtcggt tccggcggca
    12061 ttgagtccaa gaatatttcc ggtcattgtg ttataaaaag taccagttgt gacaataaaa
    12121 attccgtagc tgtaatttcc gctgagcaca ttgttgataa tcggattatg gttggaaccg
    12181 ccatccacgc agattccggt ggcattgggc aatgcgttgt ttcctgatgc atcaacgccg
    12241 atataattcc cgatcacagg attgtagctg caatttccgt aatataccat cccatacaca
    12301 cggttgcccg acaccacatt gcgctctgct ggcgtgtatc caccaagacg gttgtgtttt
    12361 gacacagtgt tgaattcaac accattgctc tgcagcaaag tgtcaccgcc gattctgaaa
    12421 gcatcgagtc cggtggaatc gggtccgaaa aaattattga caagcacatt gctgtctgca
    12481 gcttcgatgt acacccccat ttcgagattt ccggaaatga tgtttctttc agcgggcaat
    12541 gtccctccga tgatattgct gttggcactg ctcagaatat tgatgcctga atagttgtgc
    12601 agcggcgcca caccagtgat gtcgggacca ataaaatttc ctttcaccac attattgcgt
    12661 gtaccagttc cattcatgac aatggctgcg ccgcggtttc ctgaaataat attccggtga
    12721 ctaaacgttg agccgccaat ggttgtacta ctggcatacg acaaaataat tccgctttca
    12781 ttcggcaagc tgtctgttcc atacacatca gttcctatgc ggtttccaaa gatctgatta
    12841 ccactgctcg gcaaaccatc aataacaatt ccagctgtag tgtttccgga aatcaagttg
    12901 aattcgccgg acacaagact tccaatgata ttcgaagatg cattctgaat ggcaatacca
    12961 aaattgtttg acaccgcaaa agtgccacta ctgtctgttc cgatgcggtt attttttatc
    13021 gtattgttgt ttgcacctgt aatggcaata cccgcaatgg tgttacctga aacaacattg
    13081 ttgctgatga tatttgcgga tgctccgttt gcaataccaa cgccatacgt gttgggtatc
    13141 gctgccggcg cggtacccaa gtgattggtt ccaatataat tctgtgtaat aatatttccg
    13201 gttgctgttg aattatatat cattatgcca taattaaatc cctgaataat aaaacccttg
    13261 atcgtatttc caggtgaaac cagcataagc ggataatcaa atgttccagc accttttgct
    13321 aaaactattt cataaccgtg aacattggta ttcccttggt tcgtttgctg cgaagtcgca
    13381 tcaatattga tgtagccggt agtaatcata ggcagcgatg agagaagtgt tattgtgaat
    13441 gtgccggaca atgcatcaaa gttagggtca gttgtcggga taccgaaata aatattatcg
    13501 gccccaacag aagcattggc cgaagtgatg gcttcgcgca atgagccggc accgttgtca
    13561 ttggtgttga ttaccgtgta gttggctgcc cgagtaccaa aacagcataa caaaaaaaac
    13621 accccgcaca cgaattgtct cataaaaatg catttaaaca tgcaaggtaa taaaatatat
    13681 atttcatcat atctgattat ttagggtctg ctgaaaaagt ctgaggcgcg ccagatttga
    13741 ggcgacaaga cgaag
//
LOCUS       DAOPKE010000072        13445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4821_length_13445_cov_11.849365, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000072 DAOPKE010000000
VERSION     DAOPKE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_13445_cov_11.849365"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKY63_09300"
     CDS             complement(<1..619)
                     /locus_tag="PKY63_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00849.1"
                     /translation="MATHPAWALKHKKKGTELRNIRGHYYLYEVTSKWDPELKRSKKI
                     TGRMLGKITKEDGFVESDKERLRKQSLKVENLKVKEFGISNYIEKQLPIYTKLLKKHF
                     PEHWKTLIGLVYGRLVYQAPLKNMSFHFHHSYLSELHEGVDLSAKTLSKTLNEIGYQR
                     EKITDFFKEFNADNDCILIDGTDLCSDSKKIDLCELSKSKKGVYRP"
     gene            complement(828..1286)
                     /gene="tnpA"
                     /locus_tag="PKY63_09305"
     CDS             complement(828..1286)
                     /gene="tnpA"
                     /locus_tag="PKY63_09305"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPF00850.1"
                     /translation="MANTYTQLFVHFVFAVQDRTSLIKSSWKDSLYKYIIAIINEQGH
                     KPYIINGMMDHIHLLVSMNPKQSPSDLMYHVKRSSSKWINSNRLVMGKFTWQEGFGAF
                     TLDKSQIASKIDYIKNQQQHHAKKSFREEYLEFLKESEVDYDDRYIFRDV"
     gene            complement(1409..2380)
                     /locus_tag="PKY63_09310"
     CDS             complement(1409..2380)
                     /locus_tag="PKY63_09310"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HPF00851.1"
                     /translation="MRNVIIISVACLILTACSPFKKLEVGVEQAPEIRIPGSLPKVAI
                     VNMVASEASSKTYIETDFLYDGAARDGSNAICDAFLNRLGKSGKFSMQIVQYKGDDRN
                     EHREPFPMKAEQAEKFLSDLNADVLFVVEYFFADLKREVNESTNTDAEGKPVFVAKMS
                     GRVEASINIFYKDENSAIQVLKPVDYAQNIDISIAGSSKNDAKSNLGSSRTIVSNWGS
                     DIGRLQADRFVARSYDVKRDFYVGKIDEFKQAYKLLKEGNADEAIRLVTLLSGSASKQ
                     IRDQVWFNLAIIAEVQGDRNKALEYVSKSDLREAYSYKSALKALLGE"
     gene            complement(2392..4200)
                     /locus_tag="PKY63_09315"
     CDS             complement(2392..4200)
                     /locus_tag="PKY63_09315"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPF00852.1"
                     /translation="MKQTLIEKIIANHSNQKTVKPGDIVDVFIDTRAARDFGGANVVK
                     NIVDNGLKVADPAKTVFTFDCNPTGSDQKYAANQHYCRLFARDNNIKVYDIDSGIGTH
                     LAIEKGLVWPGSTFVSTDSHANIMGAIGSFGQGMGDQDIAATWAKGSVWFKVPASVKL
                     VFKGKKPDNVSAKDMVLNLLSIFGANKLLGFSVEFYGEAIDELSLDERITIASMGTEM
                     GAIIVMLTPTEKIIHEIEKLSGKKFEIISADADAEYVMTQEIDVASFKPMLALPGHPH
                     DNESVEVKKGIKIDSAFIGSCTNGRMEDMRVAAAILKGRKVAPGVVMKIVPSTNDIWN
                     QCLQEGLMKIFKEAGVLVSNAGCAGCAAGQVGQNGPDEVTLSTGNRNFAGKQGKGLVY
                     LASPAVVAASAVAGFITTPDAIPEKPAEFSNTGKFDLAKAVAKERAAKPNVVEGKVWF
                     IEKDDIDTDMIFHNRYLTITDIKEMGQYSFDNLKGYEDFAKRANPTDIVVTTKNFGAG
                     SSRQQAVDCFKSLGISCIIAESFGAIYERNAINAAMPILTYKPGQMQELGLKDGDTIS
                     VNFETGEIVNKTSGKTVKINPFYPVQLEIYQNDGLL"
     gene            complement(4373..6454)
                     /locus_tag="PKY63_09320"
     CDS             complement(4373..6454)
                     /locus_tag="PKY63_09320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPF00853.1"
                     /translation="MKKFFFVLVLPFILAACCQPCKEEAKSDVIGQQDVKIENGIMTP
                     ESMWAMGRVSDIQVSPGDSLILFGVTWYDWKQNKSNRDLYTMNPDGSNRKNITNTATG
                     EYNAVWNSKSEILFMTVNEKDEMQIYKMKSDGSARTQVSSDSGGVSGFILSPDEKNIA
                     YTKDLALPPVQDIYKDLDKANARIIDDLMFRHWDEWTTTQSHLFVAPLTDGKMGEGKD
                     LLKGEPYEVPQKPFGGLEQICWAPDGKSIVYTCRKKSGLDYSLSTNTDLYLYDLATGK
                     TKNLTEGMNGYDWNPVFSPDGKKLAWESMQRDGYESDKLRLFVLDMASGKKAEYTKSI
                     DQSAEQLRWSSDGKTIYFLSDWQGTRHIYSLNLTDTAITQISKGLCDYVAFEISGDKF
                     IAQRHSISKPDEIYSVDIKTGADKEISFINKDLLAQITMGKVEERRVKTTDGKDMLTW
                     VIYPPHFDSTKTYPVILYCSGGPQGMVGQFWSYRWNFQMMAANGYIVVAPNRRGTTGF
                     GQEWVEQISGDYGGQNMKDYLSAIDDVSKETWADENRMASVGASYGGFSVFWLAGHHE
                     GRFKAFIAHDGMFNLESQYLETEELWFVNWDLGGPYWDKNNAVAQMSYANSPHLFVDK
                     WDTPILIFHGERDYRIAYTQAMQAFTAAKVRGIPSRLVLFPEENHWVLKPQNAILWQR
                     EFKGWLDKYLK"
     gene            complement(6461..7480)
                     /locus_tag="PKY63_09325"
     CDS             complement(6461..7480)
                     /locus_tag="PKY63_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00854.1"
                     /translation="MRIKYLIFSIIALALSSAMISCGNKNDQNVQQSQDSALSPLEIL
                     NMKIEENPKADSLYQQRAELFLSMGETEKALSDVRQALQLNPEKTDYHILLGDVYLAM
                     GNIESCRKSLMKAFDMDPGNPEPSLKLAELNLFLEDYEKVFLYSNKAIDIDKYNAKAH
                     FIKGFALLEQKDTAKAIVSLQKATENDPEYYDAFIMLGHVFDLKGDPIAGNYLKTAVR
                     IRPTSAEARYNYGLWLQEQQMIEDALVQYDALLVIEPKNKNAWYNIGYINLVYLENFN
                     TAIEKFTKSIECDPVYAEAWYNRGLAYEELQDYKNARENYSQALKLKHNYENAIVALN
                     RIEGK"
     gene            complement(7477..8496)
                     /gene="recA"
                     /locus_tag="PKY63_09330"
     CDS             complement(7477..8496)
                     /gene="recA"
                     /locus_tag="PKY63_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPF00855.1"
                     /translation="MSKEKKEENPVLSEKLKALQMTIERLDKTYGKGSVMKLGDAPIE
                     ELEVISTGSVGIDLAIGVGGFPRGRVVEIYGPESSGKTTLAIHAIAEAQKKGGIAAFI
                     DAEHAFDINYAKKLGCDVENLLISQPDNGEQALEITENLIRSGAIDIIVIDSVAALTP
                     KSEIEGEMGDSKMGLHARLMSQALRKLTGTISKTRCVCIFINQLREKIGVMFGNPETT
                     TGGNALKFYASVRIDIRRISQIKDGDSILGNRVRVKVVKNKVAPPFQQAELDIIYGQG
                     FSKAGEVVDMGVDLNIIKKSGSWFSYEETKLGQGREAVKALLLDNPELMDELEKKIKE
                     KLSAQ"
     gene            complement(8504..8956)
                     /gene="bcp"
                     /locus_tag="PKY63_09335"
     CDS             complement(8504..8956)
                     /gene="bcp"
                     /locus_tag="PKY63_09335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPF00856.1"
                     /translation="MEKGMQAPDFTFTDREGKQKMLSDLRGRKVILYFYPKDSTPGCT
                     AEACDFRDNYSMWLKKGYEIIGISADSDASHVKFVEKYSLPFPLVADTEKSIIKAYGV
                     WGPKKFMGRTFDGILRTTFIIDAEGIIEEKITKVDTKASTAQILEFLK"
     gene            9324..10856
                     /locus_tag="PKY63_09340"
     CDS             9324..10856
                     /locus_tag="PKY63_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPF00857.1"
                     /translation="MKNGDFLYEPRHYNSIEIWKNVTSKQWNNAIWQQKNSIRTVEQL
                     RQVIKLTEFQASEIERTLQTMRNEGKEPMRITPYYASLMQEDPFHPKMLEGEKEKHRL
                     DPIFWQSVPTPANLLFPDTGAEGAMSEGSRSFGAAYQRYPNRIALFVAQNTSCASYCV
                     HCQRAKSLDCTNEVNKTEIDKGLFYISQNSNINEVLVTGGDALMVSRQRLQYILEELS
                     RIEHLRVIRIATRVPVVMPMAVTDDLLELINTASNRYNKGVEKYVYFMTHINHYHEIT
                     AEMAACVRRIRNHGFTIRNQTVLLNHVNDYFKTLAETFRRMFWIGVHPYYLLQCHKEK
                     GIVHFITPVQVGKIYIKHLQGWISGITMPRYAVNIEGGGGKVLLMPSGHDTLNSNESI
                     DEKISESFAIISTWDGKTIQQYESLGRAQRKEYEEAVEIMDRFIGRKKVFFPKIIIVD
                     EKGKHIETTNRTKLPLIEKMKKSELLEYSIQENDMPLTNPADIATELEENFKKSKYRK
                     QQ"
     gene            10856..12007
                     /gene="nhaA"
                     /locus_tag="PKY63_09345"
     CDS             10856..12007
                     /gene="nhaA"
                     /locus_tag="PKY63_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPF00858.1"
                     /translation="MLATRLFREFCMCEKGSGMLLIIATFLSILISNSFIGDSYIAIW
                     QIKLGMMSIEHWINDGLMAVFFLMIGLELEREIYKGELSNPRDALLPIFAAIGGMAVP
                     AAIYAFFNAGTATSGGFGIPTATDIAFALAILMMLGDRVPVSLKVFLTALAVIDDLGA
                     IIIIAIFYTNTILWPALIAALAIFVLLLIFRLFKIKHLLIYLSGGVFMWYFMLQSGVH
                     ATIAGVLLAFVIPFGKGDEISPSWKLQHAIHLPVSFGIVPLFALANTAIIINPDMGRI
                     FSEYYAIGVSAGLIIGKPLGIILFSFLSVKMKLARMPEDINWNLLLGSGFLAGIGFTM
                     SIFITILAFKDPAIISNTKFIILISSLLAGSIGFLLIRNTTRTKKKKTC"
     gene            12111..12527
                     /locus_tag="PKY63_09350"
     CDS             12111..12527
                     /locus_tag="PKY63_09350"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPF00859.1"
                     /translation="MKFTRKASANWQGSGKEGKGTLSTGSTVLNNTQYSFSSRFEEGI
                     GTNPEELIAAAHAGCFTMKLSFVLGENGFTPTSLDTKSGIVFEDGKITEINLKLTGVV
                     PGIDAGKFQELAAIAKETCPISGLLNTKINLEATLA"
     gene            complement(12695..13240)
                     /locus_tag="PKY63_09355"
     CDS             complement(12695..13240)
                     /locus_tag="PKY63_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00860.1"
                     /translation="MKNLKFFAVILFVMGMIAASCEGPEGPRGPAGTNGTDGTNGTDG
                     INGTDGINGTDGNANVMVFGFPGDTMTSSNTSLLFQLPITSTLMDSSLILPYYRDYNW
                     YQAGSMGMSGDYETRYWYRISNQTVSLNIQNPDGSDYTGADVIWDSVRIFVIPASQFY
                     AAEPDVDFANYYEVESYFGQK"
BASE COUNT         3891 a         2987 c         2558 g         4009 t
ORIGIN      
        1 atggccgata aacacctttt ttacttttac tgagttcgca aagatcaatt tttttcgaat
       61 cgctgcaaag gtcggttcca tcaattaaaa tgcagtcatt gtcggcgttg aattctttga
      121 aaaaatcagt tattttttct ctttgatatc cgatttcatt aagcgtttta ctcaatgttt
      181 ttgcagataa atcaactccc tcatgcagct ctgacaagta actgtgatgg aaatggaaac
      241 tcatgttttt tagtggagcc tgatacacca gtcgcccgta aaccagtcct atcaacgttt
      301 tccaatgttc gggaaaatgt tttttcagca gtttggtata aatcggaagc tgtttttcga
      361 tataatttga aataccaaat tccttcacct taaggttctc aactttcagc gactgcttgc
      421 gaagccgctc cttgtctgat tctacaaagc catcttcttt ggtgattttt ccaagcatac
      481 ggcctgtaat ttttttcgat cgtttcagct ccggatccca tttgctggta acttcataca
      541 aataatagtg gcctctgatg tttctgagtt cagtgccttt tttcttgtgc ttaagagccc
      601 atgcaggatg tgttgccata atattacgtg tttacgtgat tgcaaaataa caacactttt
      661 cagcaaaaac caaattattt ctgccttttt tatcaacaat tacgtgttaa atggaaaagt
      721 tacagcttaa taaaaaacaa caagaccatt ccccgtcagg ggaataatat taatagcaaa
      781 taataggcaa ttccattttc cccgtagggg ataaacattc tcaattatta aacatctctg
      841 aaaatatatc tgtcatcgta atcgacctcg ctttctttga gaaattcaag atattcttcc
      901 cgaaaagact tctttgcgtg atgctgttgt tgatttttga tataatcaat tttagaggca
      961 atttgagatt tatcaagtgt aaatgctcca aatccttcct gccaggtaaa ttttcccatg
     1021 actaacctgt tgctatttat ccattttgaa gaactgcgtt ttacatgata catcaaatca
     1081 gaaggtgatt gctttggatt catgcttaca agcaaatgaa tatgatccat cattccatta
     1141 atgatatatg gcttgtgccc ttgttcattg ataattgcaa tgatatattt gtacaatgaa
     1201 tccttccagc tgctttttat aagcgaagtt cggtcctgga ctgcaaatac aaaatgtaca
     1261 aaaagctgtg tatatgtgtt tgccatagaa tatacatgtt tatgccctac gggcaagaat
     1321 tttttttatt gttgctatta gtattaaagc cctacgggct attatcgagc attattgcgt
     1381 agaaatcaac taccagcgat tcaaacaatc attcgcccaa aagcgctttg agagctgatt
     1441 tgtaagaata ggcctcgcgc agatccgatt tgcttacata ttcaagcgcc ttgtttcggt
     1501 cgccctgcac ctctgcaatg atggccagat tgaaccagac ctgatcacgg atttgctttg
     1561 atgcactacc tgacagaaga gtcacaaggc gaattgcttc atcggcattg ccctctttca
     1621 ataatttata agcttgttta aactcatcta ttttaccaac ataaaaatct cttttaacat
     1681 cataactgcg tgcaacaaat ctgtccgcct gtaaacgccc gatatcggaa ccccaattgg
     1741 agacaatggt tcgtgatgac ccaagatttg attttgcatc gttttttgat gagccagcta
     1801 tgctgatatc aatgttctgt gcataatcta ctggcttcaa tacttgaatt gcactgtttt
     1861 catctttata aaaaatattt atggaagctt ctaccctacc cgacattttc gcaacaaaca
     1921 ccggctttcc ttcggcatca gtatttgtcg attcattcac ttcgcgcttt aaatcagcaa
     1981 aaaaatattc aacgacaaat aacacatcgg catttaaatc gcttaaaaac ttttctgcct
     2041 gctcagcttt catcggaaac ggctccctgt gctcgttacg atcatcgcct ttatattgaa
     2101 cgatttgcat ggaaaatttt ccacttttcc caagccggtt cagaaaagcg tcgcagatgg
     2161 cattggaacc gtctcgtgca gcgccatcgt ataaaaaatc tgtttcaata tatgttttac
     2221 tactcgcttc cgaggcgacc atattcacaa tagcaacctt tggcaacgag ccaggaatgc
     2281 ggatttcggg tgcctgttca acaccgactt caagtttctt gaatggagaa caggccgtca
     2341 gaatcagaca ggcaaccgaa ataataatca cattccgcat aaattcaagg tttatagtaa
     2401 tccgtcgttc tgataaatct caagttgaac aggataaaaa ggattgattt ttactgtctt
     2461 tccggacgtt ttgttgacaa tttcacctgt ctcaaaattc acgctgatgg tgtcgccgtc
     2521 tttcagtcca agctcctgca tctgacctgg tttgtacgtc agaatcggca tggcggcatt
     2581 aatggcgtta cgctcgtaaa tagctccgaa cgattcggca atgatgcatg aaataccaag
     2641 cgacttgaag caatccactg cctgctggcg cgaggagcca gctccgaagt ttttcgttgt
     2701 aacaacaatg tcggtcggat tcgcacgctt tgcgaaatct tcatatcctt tcagattgtc
     2761 gaacgaatac tgtcccattt ccttgatgtc tgtaatggtc agatagcgat tatgaaaaat
     2821 catatcggta tcaatatcat ctttttcaat aaaccagact tttccttcaa cgacatttgg
     2881 ctttgcagca cgctccttag ctacagcttt ggcgagatca aactttccgg tatttgaaaa
     2941 ttcggccggt ttttcgggaa tcgcatcagg tgtggttata aaaccagcca cagcactggc
     3001 tgcaacaaca gccggcgaag caagataaac cagccccttg ccttgtttac cagcaaaatt
     3061 acggtttccg gtggacagag tcacttcatc gggaccattc tgtccaacct gaccagcagc
     3121 acaaccggca caaccggcat ttgagaccaa tacacctgcc tccttgaaaa ttttcatcaa
     3181 accttcttgc agacactgat tccaaatatc gttggtagat ggtacaatct tcatgaccac
     3241 ccctggagca acttttcgac ctttcaaaat ggctgcagca acacgcatat cttccatgcg
     3301 tccattggta caacttccaa tgaatgccga atctatcttg attccttttt ttacttccac
     3361 agattcgttg tcgtgcgggt gtccgggcaa tgcaagcatc ggtttaaatg aagctacatc
     3421 aatttcctga gtcattacgt attcagcatc agcatcagcc gaaataattt caaatttctt
     3481 tccactcagt ttttcaattt cgtgaataat tttttcagtt ggggtcaaca ttacaatgat
     3541 tgcacccatt tctgtaccca tagatgcaat agtaatacgt tcatccaacg agagctcatc
     3601 aatggcttcg ccataaaatt caacagaaaa accaagcaat ttgttagcac caaagattga
     3661 tagaagattc aaaaccatat ccttggcaga aacattgtcg ggttttttac ctttaaaaac
     3721 aagtttgaca gatgcaggaa ctttaaacca aactgagccc ttcgcccagg ttgcagctat
     3781 gtcctgatct cccatgcctt gcccaaaaga accaatggcg cccatgatat tggcatgtga
     3841 atcggtggac acaaatgtcg aacccggcca tacaagccct ttttcaatgg caaggtgtgt
     3901 tccgataccc gagtcaatgt cataaacttt aatgttattg tcgcgtgcaa acaaacggca
     3961 ataatgctga ttggcagcgt acttctgatc ggaaccggtc gggttgcagt caaaagtaaa
     4021 aacggttttt gcgggatcgg caactttcag tccgttgtcg acaatattct ttacaacatt
     4081 ggcgccgccg aaatcgcgcg cagcgcgggt gtcaataaag acgtcaacaa tatctccggg
     4141 ttttactgtt ttctgattgc tgtggttagc aatgattttt tcaataagtg tttgtttcat
     4201 atctgtgttt tttggaagtc tattgtgcaa aaagctccat cctttgatta agtctatcct
     4261 gctgcctcct tcgatacgtc gcaagtcttt tgttgaaaaa gcgcgacaag agcgacactc
     4321 aggatgacat aaggcaggac tcaggatgac attttactgg ttcatagaaa aactatttca
     4381 agtatttgtc aagccagccc ttgaattctc gctgccataa aatggcgttc tgcggtttca
     4441 gaacccaatg gttttcttca ggaaaaagaa caagacgcga cgggattcct cggactttag
     4501 cagcagtaaa ggcctgcatg gcttgcgtgt atgcgatacg gtaatcacgt tcaccatgga
     4561 aaatcagaat cggtgtatcc catttgtcaa caaaaagatg cgggctgttg gcataggaca
     4621 tctgcgcaac cgcattgttt ttatcccagt atgggccacc caggtcccag tttacaaacc
     4681 agagttcttc cgtttcgaga tactgacttt caagattaaa catgccgtcg tgagcaataa
     4741 aagctttaaa gcgaccttcg tgatgacctg ccagccagaa aacagagaac cctccgtagc
     4801 tggctcccac tgaggccata cgattttcat cagcccaggt ttcttttgaa acatcatcga
     4861 tggccgacaa ataatctttc atgttttgtc caccgtaatc gccgctgatc tgctctaccc
     4921 actcctgccc aaagccagta gtgccgcggc gattgggagc aacaacaatg tatccgttgg
     4981 cggccatcat ctggaaattc cagcggtagc tccaaaattg tccgaccatt ccctgtgggc
     5041 cacctgagca atagagaatc accggatatg tttttgttga atcgaaatgc ggcggataaa
     5101 taacccaagt gagcatatct tttccatcag tcgtttttac ccggcgttct tcaacttttc
     5161 ccatggtaat ctgtgccagc aaatccttat tgatgaaaga aatttccttg tcggcaccgg
     5221 ttttaatatc aacagaataa atttcatccg gtttggaaat actatgtcgc tgagcaatga
     5281 atttgtcgcc ggaaatttca aaagcaacat aatcacacaa tccttttgat atttgcgtaa
     5341 tcgcagtatc agtaaggttt agagaataaa tatgacgcgt tccctgccag tcacttaaga
     5401 aataaatagt ttttccatcg ctactccaac gcaactgttc ggcgctctgg tcaatacttt
     5461 tggtatattc agccttttta cctgatgcca tatcaagaac aaaaagtctt agtttatctg
     5521 attcataacc atctcgctgc atactttccc aagccagttt tttgccatcg gggctgaata
     5581 caggattcca gtcataacca ttcattcctt cagtcagatt cttggttttt ccggtagcaa
     5641 gatcatataa ataaagatct gtattggtgg acaaagaata atcaagtccc gatttcttac
     5701 gacaagtata aacaattgac ttcccatcag gagcccagca aatctgttca agtcctccaa
     5761 aaggtttctg tggcacctca taaggttccc ctttgagcag atctttgcct tcgcccatct
     5821 tgccatccgt taatggagcg acaaacaaat gactctgagt agttgtccat tcgtcccaat
     5881 gacgaaacat aagatcatca ataatgcgtg catttgcctt gtcaaggtcc ttgtaaatat
     5941 cctgaacagg gggcaaagca aggtctttgg tgtaagcaat atttttttcg tccggggata
     6001 agatgaaacc cgaaactcca cctgaatcgc tggaaacctg cgttcttgca gaaccatctg
     6061 atttcatctt ataaatctgc atttcatctt tttcatttac cgtcatgaaa aggatttcgc
     6121 ttttgctgtt ccagacagca ttgtattctc ctgtagccgt attggtgata tttttacgat
     6181 tacttccatc aggattcatg gtataaagat cccggttact tttattttgc ttccaatcat
     6241 accaggtcac tccaaaaagg atcagactgt cgccagggct aacctggata tcggaaaccc
     6301 ggcccatggc ccacatagat tcaggagtca taattccgtt ttcgattttt acatcctgct
     6361 gaccaatgac atcgcttttt gcctcttctt tacagggctg acaacaagcc gcaagaatga
     6421 acggcaatac aagaacaaaa aagaattttt tcatggcaaa ttattttcct tcgattcggt
     6481 ttaatgcaac aatagcgttt tcgtaattgt gttttaattt caacgcctgc gaataatttt
     6541 cacgggcatt tttataatcc tgcaattctt catatgccag acctctatta taccatgctt
     6601 cggcataaac agggtcacat tcgatagact tagtaaattt ttcgatggct gtattaaaat
     6661 tctccagata aaccagatta atgtatccga tattatacca ggcatttttg ttttttggtt
     6721 caataacaag taaagcatca tattgaacca aggcatcttc aatcatttgc tgttcctgaa
     6781 gccaaagccc gtaattgtag cgcgcctcag cagatgttgg tctgatacgg acagccgttt
     6841 tcagataatt cccggcaatg ggatctcctt tcagatcaaa cacatgcccc agcattataa
     6901 aagcatcata atattccggg tcattttcag ttgccttctg cagacttaca atagcttttg
     6961 ctgtatcttt ctgttcaagc aacgcaaaac ccttgataaa atgagccttt gcattgtact
     7021 tgtcgatatc aatcgcttta ttgctgtaca aaaacacttt ttcgtaatct tccagaaaaa
     7081 ggttgagttc agccagttta agactcggtt cagggtttcc cggatccata tcaaaggcct
     7141 tcatgagaga ttttcggcag gattcgatat tccccatagc aagatacaca tcgcccaaaa
     7201 gaatatgata atctgttttt tcgggattca gctgcaatgc ctgccttaca tcgctgagcg
     7261 ctttttcggt ttcacccatc gataaaaaca attcggcccg ttgttgatac aagctgtcag
     7321 ctttggggtt ttcctcgatt ttcatattca gaatttcaag tggagacaaa gcagagtcct
     7381 gcgactgctg cacattttga tcgtttttgt ttccacacga aatcatcgcc gaggacagag
     7441 ccagagcgat gattgaaaaa atcagatatt taattctcat tgagcgctga gtttttcttt
     7501 aattttcttt tcaagttcat ccatcaattc aggattatca agcagcaagg ccttgacagc
     7561 ttcgcgtcct tgtccgagtt tcgtttcctc ataactgaac cacgaaccac tttttttgat
     7621 aatattcaga tcgacaccca tgtccacgac ttcacctgct ttcgaaaatc cctgtccata
     7681 aatgatatcc agttcggcct gctgaaatgg aggagcaacc ttatttttga caaccttaac
     7741 cctgactctg tttcccaaaa tgctgtcacc gtctttgatc tgcgaaatac ggcgaatatc
     7801 gatacgcaca gaagcataga atttaagggc atttccaccc gttgtagttt ccggatttcc
     7861 aaacataaca ccgatttttt cacggagctg attaataaaa atacacacgc agcgggtctt
     7921 tgaaatagtt cctgtaagtt tacgcagggc ctgcgacatc agacgggcat gcaaacccat
     7981 tttggagtcg cccatttcgc cttcaatttc gcttttgggt gttaaagctg ccacagagtc
     8041 aataacaata atatctatag caccgcttcg aataaggttt tcagttattt caagtgcctg
     8101 ctcgccattg tccggctgcg aaataagcag attttcgaca tcgcagccaa gctttttggc
     8161 ataattgata tcaaatgcat gttcggcatc gatgaaagcc gcaatgccac cttttttctg
     8221 cgcttcggca atggcatgaa tggccagtgt ggttttaccg gaactttcag gaccataaat
     8281 ctcaacgaca cgtccccgcg gaaaaccgcc aaccccgata gccagatcaa tgccaacact
     8341 tcccgttgaa ataacttcga gttcttcgat gggagcatcg cccaacttca tcactgatcc
     8401 ttttccatac gtcttgtcaa ggcgctcaat ggtcatctgc aacgctttca gtttctcgga
     8461 taaaaccgga ttttcttctt ttttctcttt actcatatcg tgtttatttt aaaaattcaa
     8521 gaatttgagc cgtactggct ttggtgtcaa ctttagtgat tttttcttca ataattcctt
     8581 cggcatcaat aataaatgtt gtccgcaaaa taccatcgaa ggtccgaccc ataaattttt
     8641 tcggtcccca aacaccataa gctttaataa tgctcttttc ggtatcggcc accaaaggaa
     8701 aagggagtga atatttctcc acaaacttca catgcgaagc atcactgtcg gcgctgatgc
     8761 caataatttc gtatcctttc ttcaaccaca tgctgtaatt gtcgcgaaaa tcgcaggcct
     8821 cggctgtgca gcccggggtt gaatccttcg gataaaaata cagaattact ttgcgccctc
     8881 tcagatcgct cagcattttc tgttttcctt cacggtcagt aaaggtaaaa tcgggtgcct
     8941 gcatgccttt ttccatagcg gagaaatttg gtttaaaatt actacttata atgtaatgca
     9001 caagaaattt gttttcaaca tttcgccgac aaaattattt tcttcgttta aaccaagccg
     9061 gttaataagt acttactttc gtttgtaaac ggatttaatt gattgtaacc gtttgttgtc
     9121 ggatttatat ctgattatca actaaataaa ttcatgaaaa ttacggaaac atttgataca
     9181 gtttgttcat ttccaaaaaa ttcattatct tcgtctgagc tttaacattt gtcttagtca
     9241 ttgtctgcaa acgattcttt gtcatgaatt gagacaaata gcaatgaaac aataccactt
     9301 taattcagaa cagaaaggat agtatgaaaa atggggattt cctgtacgag ccaagacatt
     9361 acaacagcat tgaaatatgg aaaaatgtaa cgtcaaaaca gtggaataat gcgatatggc
     9421 agcaaaaaaa ttcgatcaga accgttgaac aactgcgaca ggttattaaa ctgacagagt
     9481 ttcaggcatc ggaaatagaa agaactttgc aaaccatgcg caatgaaggc aaagagccca
     9541 tgcgaataac accctactat gcctcgctga tgcaagaaga tccatttcat ccaaagatgc
     9601 tggaaggaga aaaagagaaa catcggcttg acccgatttt ttggcaaagt gtaccaaccc
     9661 cggccaatct gctatttccg gatacgggcg ccgaaggagc aatgagcgag ggcagccgca
     9721 gtttcggtgc cgcatatcag cgatatccaa accggatagc actttttgta gctcaaaaca
     9781 ccagttgtgc atcttactgc gttcattgtc aaagagcaaa atcgctggat tgcaccaacg
     9841 aagtcaataa aacagaaatt gacaaaggac ttttttatat ttcgcaaaac agcaatatca
     9901 acgaagtact tgtaaccggt ggcgatgcat tgatggtaag ccgtcagcgg ctgcagtata
     9961 ttcttgaaga actcagcaga attgagcatt tgagggttat ccgcattgca accagagtgc
    10021 ctgtagtaat gcccatggct gtcactgacg atttgcttga gctgatcaac acagcatcga
    10081 accgttacaa caaaggagtt gaaaaatatg tatatttcat gacacacata aatcattacc
    10141 atgaaataac ggccgagatg gctgcatgtg tgcggcgaat cagaaatcac gggttcacta
    10201 tccggaatca gacagttctg ctcaatcatg tgaatgatta tttcaaaaca ctggcagaaa
    10261 cattcaggcg aatgttctgg atcggtgtcc atccctacta tttgcttcaa tgtcataaag
    10321 aaaaaggaat tgttcacttt atcacacctg tgcaggttgg aaaaatctac ataaagcatt
    10381 tacagggctg gatttcaggc attaccatgc ctcgttatgc tgtaaatatt gaaggaggtg
    10441 ggggcaaggt gcttctgatg ccttcgggcc atgacacgct gaacagcaat gaaagtatcg
    10501 atgagaaaat ttcggaaagt tttgccataa tcagcacctg ggatgggaaa accatccagc
    10561 aatatgaatc gctaggacgc gcccagagaa aagaatatga agaagccgtt gaaattatgg
    10621 atcgctttat tgggcgtaaa aaagtgtttt ttccaaaaat aatcattgtg gatgaaaaag
    10681 gaaaacacat tgaaacgacc aacagaacca aacttccttt aattgaaaaa atgaaaaaat
    10741 cggagttgct ggaatactcg attcaagaaa acgacatgcc attgaccaat ccggcagata
    10801 ttgccaccga gcttgaagaa aattttaaaa aatcaaaata cagaaaacaa caataatgct
    10861 tgcaacaagg ttattcagag aattttgcat gtgcgaaaaa ggcagtggta tgctgctgat
    10921 tattgcgaca tttttatcca ttttgatttc aaattcattc attggagata gttatatagc
    10981 catctggcag ataaagctgg gcatgatgag tatcgaacat tggataaacg atggactgat
    11041 ggctgtgttt tttctcatga ttggtctcga gttggagcgc gaaatataca aaggagaatt
    11101 gtctaatccg cgcgacgcct tgttgcccat atttgcagct attggtggta tggcagtacc
    11161 tgcagccatt tatgcatttt tcaatgctgg tacagcaacc agtggagggt ttgggattcc
    11221 caccgccacc gacatagcat ttgcactggc cattctgatg atgctgggcg acagggttcc
    11281 ggtctcgctc aaggtttttc tcacggcatt ggctgttatt gatgatcttg gagccatcat
    11341 tattattgcg attttctaca caaacaccat tttgtggcct gcgctgattg ctgctttagc
    11401 aatattcgtg ctcttgctca tcttcagact ttttaagatt aagcacttat tgatttatct
    11461 cagcggcgga gtattcatgt ggtatttcat gcttcaatct ggggtacatg caaccattgc
    11521 cggcgtatta cttgcttttg tgattccatt tggcaaaggc gatgaaatct caccttcatg
    11581 gaaactccag cacgccattc atctaccagt ttcatttgga attgttccac tttttgcact
    11641 tgccaacact gccatcatta tcaatcctga tatgggtcgg attttttcag agtattacgc
    11701 cattggagtc tctgccggac tgataattgg caagccgctc ggaatcattc ttttcagctt
    11761 tctatccgtt aaaatgaaat tggcacggat gcctgaagat attaactgga atttgttgct
    11821 ggggagcggt tttctggctg gaattggttt cacaatgtca attttcatca ccatcctggc
    11881 attcaaagac ccggcaatca tcagcaatac aaaattcatc atcctgatat cttctttgct
    11941 tgctggcagc attggatttc ttttaatccg taacacaact cgtaccaaaa aaaaaaaaac
    12001 atgctgacaa aagctgataa taatcatttc ggatcttaaa acccacctgc aagtattatt
    12061 tttgcataaa aagtttcaca gctataaacc acttaaaata caaacaaacc atgaaattca
    12121 cacgcaaagc aagcgccaac tggcagggat caggaaaaga aggcaaagga acactttcaa
    12181 ccggcagcac cgttttaaat aacacccaat attcattcag ctcacgattt gaagaaggca
    12241 taggaacaaa tcctgaagag ctgatagcag ctgcacatgc gggatgtttt accatgaaac
    12301 tcagctttgt tctgggcgaa aacggtttca ctcctaccag tctcgatacc aaatcgggca
    12361 ttgtgttcga agacggaaaa atcaccgaaa tcaatctgaa actcaccggt gttgtgcctg
    12421 gcatcgatgc cggaaaattt caggaactgg cagccattgc caaagaaaca tgtcccattt
    12481 caggactgct gaatacaaaa atcaatctcg aagcaacatt ggcataaaac cctatacgat
    12541 caacaataca taaccctaca atcaggcgcg gaattttctg cgcctttttt ttgccaacaa
    12601 atattttgtt caatacttga aagtcaaatt ccttaaatag gaaaaagccc gccacaacca
    12661 ttgcgacggg ctctttaaac aataaaataa ttgcttattt ctgaccgaaa tagctttcta
    12721 cttcgtaata gtttgcaaaa tcaacatccg gctcagctgc atagaactga ctggctggaa
    12781 tcacgaaaat gcgcacagaa tcccatatta catcagcgcc agtataatcg gatccatcgg
    12841 gattctgaat gttcaatgaa accgtttgat tcgatatacg ataccaataa cgggtttcat
    12901 aatcaccaga catacccatt gaacctgcct gataccaatt gtaatccctg taataaggga
    12961 gaatcaagga tgaatccatc aatgtacttg taatcggaag ctgaaacaaa agtgaagtgt
    13021 ttgatgaagt catggtatca ccaggaaatc caaagaccat cacattcgca tttccatctg
    13081 ttccattgat tccatctgtt ccattgattc catctgttcc attggttcca tctgttccat
    13141 tggttccggc agggccgcga gggccttcag gaccttcgca cgaagcagct atcattccca
    13201 ttacaaagag aataacagca aagaatttta gatttttcat aacagtcgtt tttaatttca
    13261 aatgcaaatg tacatatttt aaacttatat tcaagcattt tgtcggttat atatgattca
    13321 tttctattga atacatattt attcttactt tttaacattg ttatatagtc caataacaaa
    13381 ttgattcaca atttatgaaa taatcgcaat atttcatctg agaatttgtt acttttagta
    13441 ccaat
//
LOCUS       DAOPKE010000073        13402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4835_length_13402_cov_10.997902, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000073 DAOPKE010000000
VERSION     DAOPKE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_13402_cov_10.997902"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..337
                     /locus_tag="PKY63_09360"
     CDS             62..337
                     /locus_tag="PKY63_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF00861.1"
                     /translation="MCVLKLSDGAAKYTGDLMQELKTARLLVVEGTIYPLLNRLKDEG
                     YLEYYWEESTGGPPRKYYRCTHKGREYLSMLRQGWYEMVESVNAIIK"
     gene            346..1896
                     /locus_tag="PKY63_09365"
     CDS             346..1896
                     /locus_tag="PKY63_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPF00862.1"
                     /translation="MKKSFSVNIGYRLFNIDEDAYERLNKYLDSIKAGLDAEGADEVI
                     RDIEIRIAELLSERINVSKEVIVLADVEFVISELGEAEQIGDFSDEQKSKQQTSRNER
                     RLFRDPDDRILGGVCSGLAGFFDIDSLWVRIAFVVLFMFGGSGLLIYLILWLVVPVAE
                     TSAEKLQMKGRKVNVNNLSDRVREEFDQVRNSFRKKKGNTQTQAHTAKTGGSIVESDA
                     FQSLMYILGAIGGFFLIAFSLAILAGFALSFFADISYMVHSHDLGVSASFCDAMFFIT
                     TSRFVGWMSIVAFLLFVCIPLISMLIFGLKLIFRFRFPMKWYNRIASSLWGISFVALA
                     ALTIYHVVYYSNSVNESKTIIIPKTNKTIQLSVDGKTPDVFNSIGSADTRWYFSENNG
                     KMQLLLQTELEIEPEMYDSNIRIVAHLRNAGDLTTLAYSDWVPMVQDSSQIIIPEWLP
                     SGQGSFHISEVEVYIPVGTQISLTPETRQLLNAQNELDNVLMTYSDFVMTSDGLRPAL
                     QAVEPVVK"
     gene            1994..2761
                     /locus_tag="PKY63_09370"
     CDS             1994..2761
                     /locus_tag="PKY63_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF00863.1"
                     /translation="MPNKITFLGTGTSTGVPVLTCECEVCRSDNPMDKRLRTSAYIEC
                     NGLRLIIDCGPDFRQQLLTNNIRDFDAVLLTHGHRDHIAGLDDIRAFNYIRGKTIDMY
                     ANQDTVESVKTEFPYIFNPGDYLGAPKINLAVVDDHPFEIKGLPVIPIPVMHGDASIL
                     GYRIGNMVYITDASFIPESSMDLIKDADLLVLNALRKKKHPSHFSLDEALEHIEKLRP
                     KAAYLTHISHFLGLHTEVQPKLPAGVFLAYDGLTVEC"
     gene            2826..3233
                     /locus_tag="PKY63_09375"
     CDS             2826..3233
                     /locus_tag="PKY63_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00864.1"
                     /translation="MKKLLLPLIVIMLVFTGCYYEEGPIISLRTPENRLVNKWKYEKF
                     SLNGQDQMQHYQSSWIQFNSDNTAMFYEDTSYSYTAAWEFSDDFKTLYLNCSDDSLNT
                     WEIDYNILKLRDKELWMESDMGSITMYIELSEY"
     gene            3256..3918
                     /locus_tag="PKY63_09380"
     CDS             3256..3918
                     /locus_tag="PKY63_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00865.1"
                     /translation="MKHFASVMAMAFVAFILISCGGKESKTTDQTDTTKTEEKVASGN
                     PDQIYGVEKGMIEYKMVTMGMPTTMTQYWDEYGKKSYTQSSMEMMGVTTITHMLNDGE
                     FIYSWDATTKEGTKAKVGKSQNLNYNDMSEEMMAKYHMTKEGTEEVNGKTCDVYKMEM
                     EGTKSKTWVWKGIAIKTETMAMGMTVTTEMTKLEEGADAPEGAFEVPAGIKFNEISAP
                     AR"
     gene            4080..5993
                     /gene="dnaK"
                     /locus_tag="PKY63_09385"
     CDS             4080..5993
                     /gene="dnaK"
                     /locus_tag="PKY63_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPF00866.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGSEPIVIANSEGKRTTPSMVAFTD
                     NGERKVGDPAKRQAITNPTRTVYSIKRFMGETFDRVGKEIARVPYKVERGPNNTPRVL
                     IDDRQYTPQEISAMVLQKMKKTAEDYLGIEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LTVRRIINEPTAAALAYGLDKKNRDIKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDQRIIEWLAEEFKKDEGLDLRKDPMALQRLKEAAERAKIELSSSAQTEIN
                     LPYIMPVDGMPKHLVKTLTRARFEQLIDDLVKATIEPCRKALSDANMKNTDIDEVILV
                     GGSTRIPVIQQVVEEFFGKKPSKGVNPDEVVAVGAGIQGAVLTGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIESNTTIPTKKAETFSTAADSQTSVEIHILQGERPMANQNKSIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKSQQIRIEASSGLTDDEIKK
                     MKAEADANADADKKERERIDKINQADSMVFNTEKQLKEYGEKIPADKKSEIETALTEL
                     KEAHKAQNIEAIDAATARLNTAWQAASEEMYKNTGPQGGPEAGADPGAQKQQQNSGSD
                     EVTDVDFEEVKDK"
     gene            6095..7309
                     /locus_tag="PKY63_09390"
     CDS             6095..7309
                     /locus_tag="PKY63_09390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPF00867.1"
                     /translation="MIPFAPPRIDQKIIDAVNEVLRSGWITTGPRTREFESMLKDYAG
                     CSEVLCINSASAGLELILRWFGVGPGDEVILPAYTYAATANVVIHCGATPVFSDSSPV
                     DFNITVDEIRKNITAATKVIVPVDLAGFPCDYYAINNLVDDPDVSSLFSPDTDNQKKL
                     GRILVLSDAAHSIGATYKGKRTGALTDISVYSFHAVKNLTTAEGGAICLNMKAPFENN
                     LIKQDLMLKSLHGQNKDAQAKLIPGNWKYDIIEPGYKCNMTDIQAAIGIVELKRYDSD
                     MLARRRLIFSMYAQLLQNHPRVQLPVYKTDVAESSYHVFLLRIKGITEQQRDDLIQEI
                     FRREVAVNVHFTPLPLMTYYKNAGYNINDYPVAYDNFSREISLPVYYDLSNEQVQTVA
                     KTVIDSINSICP"
     gene            7306..7905
                     /locus_tag="PKY63_09395"
     CDS             7306..7905
                     /locus_tag="PKY63_09395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPF00868.1"
                     /translation="MIGKRLFDLFFSFFSILILLIPGLILALIIVIESRGGAFYRQVR
                     VGRNEKLFRLMKFRTMFSGSDSKGLLTVGSTDSRVTGFGRFLRKSKMDELPQMLNIFA
                     GHMSFVGPRPEVPKYVAMYSDEQKNVLRVRPGLTDYASLEYINESDLLAKQSEPERFY
                     IETVMPAKLSLNLKYIKEMSCVTDMKIIFRTLFKIGRRR"
     gene            complement(8098..9387)
                     /locus_tag="PKY63_09400"
     CDS             complement(8098..9387)
                     /locus_tag="PKY63_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF00869.1"
                     /translation="MKLRKHTGKNNHQEFEGLGTSGKGNPFTTPPGYFDGLSSDIMKT
                     VGTVTILALLRTKPLQVAIGVVTTIAVATAIYFFSSDSSKQNNNSSTVNKNYILPDVR
                     LDENAEQPISVVSAEDDNKQIVVVNSDISSERMIQYFNQLPVNQSLKKHLTDEYAMQL
                     ASYQTTMNEGPDDGKMLAEVIEINNETEYLIPILSNENSDNAERTLSAFEMLPRDTCS
                     EQSMTLRAQFPGKTTYLWNNGATTPEISVDKSGIYRVTIGLENGKKLSKAVSVKIIPK
                     PSLNNDYLITACTGSILRLSVGQKTGGYQYYWPQYKLSTPQLTVSKPGLYYAQITGCQ
                     TYVDSFFVVFTHCELGIPNMITPNGDGISETFSITNLSKYPNTELSIYDKSNKLIYKS
                     LNYQNDWDASLTPDGSYFFILKFTDGSTQEGLITVKH"
     gene            complement(9423..9971)
                     /locus_tag="PKY63_09405"
     CDS             complement(9423..9971)
                     /locus_tag="PKY63_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF00870.1"
                     /translation="MSKITDAEILNLLKAEKSRQRGFELLLNKYKEQAYWLIRRIVID
                     HDDANDVIQNTFIKVWQNLDKFREDSKIYTWIYRIATNEALSFLKSKKSHLFVSMDEM
                     RDDFGDRIESDIYFKGTDIQRKLQKAVATLPARQKLVFIMRYFEAMPYQDIANVVGVS
                     IGTLKASYHIAVKKIEEILKTN"
     gene            complement(9978..10940)
                     /locus_tag="PKY63_09410"
     CDS             complement(9978..10940)
                     /locus_tag="PKY63_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPF00871.1"
                     /translation="MKPIEVRQKKDTRSGFGDALADLGKKNEKVVALCADLTGSLKMN
                     TFAANFPERFFQTGIAEANMIGMASGLAIAGYIPYTGTFANFSTGRVYDQIRQCVAYS
                     NKNVKICASHAGITLGEDGATHQIMEDIALMRALPNMTVINTCDYSQTYMATQAIAEW
                     DGPVYLRFGRPVVPDFSPIDTPFQIGKGIVFTEGTDVTIVATGHLVWEALEASRILET
                     KGISAEIINIHTIKPLDNELLLKSISKTRCVVSAEEHVQAGGLGEAISALLSQKLPTA
                     QEYVAMPDVFGESGTPEELMVKYGLKAGNIVDAAIRALNRKTNL"
     gene            complement(10956..12341)
                     /locus_tag="PKY63_09415"
     CDS             complement(10956..12341)
                     /locus_tag="PKY63_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF00872.1"
                     /translation="MRKLSAILVFLLLAFGATAQTGTPDKEKVITTRILFLFDASQSM
                     YGRWQSDMKITIARKIMSDLLDSLRDVPNLELALRCYGHTKDYPPQDCDDTRLEVPFG
                     PDNIEKIKYKLKTINPRGTTPIAYSLEKAGGDFPPCANCRNIIILITDGLEECNGDPC
                     AVSLALQKKGIVLKPFIIGIGKSFKEQFDCVGTYFDATTEKQFSTALNIVISQALNST
                     TCQVNLLDINGNATETNVNMTFYDKLSGLPKYNFIHSLNNRGFPDTLVIDPMLNYRIQ
                     VHTIPPVFIDSLELTAGKHTIVGVSAPQGFLEVKTEGSYAQSRNSILCVVRENNKLNT
                     LNTQFLFNREKYICGLYDLEIMTLPRTYVEDVKIDQSTTTFVEIPAAGTTIFQFPASG
                     YGSLYVKRENKLEWVYNFRGSGEQEILMLQPGDYFAVFRSKYQTRSMLTIEKNFTVKP
                     AAMTKVNMSMF"
     gene            complement(12344..13189)
                     /locus_tag="PKY63_09420"
     CDS             complement(12344..13189)
                     /locus_tag="PKY63_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPF00873.1"
                     /translation="MLSVEELNKFSSQVRRDIIRMVHAVQSGHPGGSLGCTDFLVALY
                     NNVMNHNPASFSMQGTNEDVFFLSNGHISPAWYSVLARSGYFPISELGTFRKLGSRLQ
                     GHPTPAEHLPGVRIASGSLGQGLSAAIGNALGKKLSGEKTFVYTLHGDGELQEGQIWE
                     AAMFAAAKKVDNLIATIDYNGKQIDGPVDEVVSLGDLAAKWTSFGWTVLEMDGHNMQQ
                     ILNTMENAKALSGKGKPVVILMTTIMGKGVSFMEGSHKWHGSPPSDEQKNKALSELTE
                     TLGDY"
BASE COUNT         3708 a         2663 c         3080 g         3951 t
ORIGIN      
        1 gcgttcttgc aaaagtccgt attaaaccac gcaactgttc atagccgaga ccgttttgga
       61 gatgtgtgta ttgaagttgt ctgacggggc agccaaatat accggcgacc tgatgcagga
      121 attaaagacg gcccgtctgc tggtggtgga aggaaccatc tatcccttgc taaatcggtt
      181 gaaagatgag ggatacctcg agtattactg ggaggaaagt acaggcggac cgcccaggaa
      241 atattaccgc tgcacccaca agggcaggga atacctgagc atgttgcgtc agggttggta
      301 tgaaatggta gaatcagtaa atgcaatcat taaataaaaa acaaaatgaa aaaaagtttt
      361 tcggtcaaca tagggtatcg tctcttcaac attgatgaag atgcgtatga acgattgaac
      421 aaatatctcg attccatcaa agctggtctt gatgctgaag gggccgatga agtaattcgc
      481 gacattgaga tcaggatagc tgaattgctc agtgaaagaa tcaatgtttc taaagaagtg
      541 attgtgctgg cggatgttga atttgtcatc agcgaattgg gcgaagcgga gcagattgga
      601 gatttttcgg atgagcaaaa atcaaaacag cagacatcga gaaacgagcg acgtttgttc
      661 cgcgatccgg atgaccggat tttgggtggt gtttgttcag gacttgccgg atttttcgat
      721 attgactcgc tgtgggtacg cattgcattt gtggtgttat ttatgtttgg tggttcgggt
      781 ttgcttattt atctgatttt gtggctggtg gttccggtgg ccgaaaccag tgcggaaaag
      841 ctgcagatga aaggtcgcaa agtaaatgta aataatctga gtgatcgtgt tcgcgaagag
      901 tttgatcagg tgcgtaattc tttccggaag aaaaaaggaa acactcagac gcaggcgcac
      961 actgctaaaa caggcggcag cattgttgaa agcgatgcat ttcaatcgct tatgtatatt
     1021 ctcggggcca ttgggggctt ctttctgatt gctttttcgc tggccatcct ggcaggattt
     1081 gcattatcgt tttttgccga tatcagctat atggttcata gtcacgatct tggcgtttcg
     1141 gcttcttttt gcgatgcaat gtttttcatc acaacaagcc ggtttgttgg ttggatgagt
     1201 atcgttgcct ttttgttatt tgtttgtatt ccactgatat caatgctgat attcggactg
     1261 aaactgattt tccggtttcg ctttccaatg aaatggtaca accggattgc atcatcgctt
     1321 tggggaattt cgtttgttgc gcttgctgct ctgaccattt atcatgtggt gtattattca
     1381 aattctgtca atgaaagtaa aaccataatt attcccaaaa ccaacaaaac cattcagtta
     1441 tcggttgatg gaaaaacacc ggatgttttt aattcaattg gaagtgctga tacccgttgg
     1501 tatttcagcg aaaataatgg caagatgcaa ttattgttgc agactgaact tgagattgag
     1561 ccagagatgt acgacagcaa tattcgtatt gttgcccact tacgcaatgc cggtgatctg
     1621 acaacgctgg catacagcga ttgggtgcca atggtgcagg attcatctca aatcattatt
     1681 ccagaatggc tgccgtcggg gcagggcagt ttccatatca gcgaagtgga agtttatatt
     1741 ccggttggaa cacaaatttc gttaacacct gaaacaagac aattgttgaa tgctcaaaat
     1801 gaactcgaca atgttttgat gacctacagt gattttgtaa tgacatctga tggcttgcgg
     1861 ccagcattgc aggcggtgga acccgttgtg aaataatttt gaaacaacaa tgttagtaaa
     1921 atcactggta aaatcactgg cgcgatttgt aatccgtgcc agtgatttta cattttgcta
     1981 tctttgcttt gtaatgccaa acaaaatcac ttttctcgga accggcacat cgaccggtgt
     2041 accagtgctc acatgtgagt gcgaagtttg tcgctctgat aatcctatgg ataagagact
     2101 cagaacatcg gcctatattg agtgcaatgg tttgcggctg atcatcgatt gcgggcctga
     2161 tttccgtcag cagcttctga caaacaacat ccgggatttt gatgcagttc ttcttacgca
     2221 tggtcatcgc gatcatatag ccgggctcga cgacatcagg gcattcaatt atatccgcgg
     2281 taaaaccatt gacatgtatg ccaatcagga taccgtggaa tcagtcaaaa cggaatttcc
     2341 atacattttc aatcctggcg attatctggg tgcacccaaa atcaatctgg cagtggttga
     2401 tgaccatccg tttgaaataa aaggtctgcc cgtcattccg attcctgtca tgcatggcga
     2461 tgcttccatt cttggatacc gcatcggcaa tatggtgtac ataaccgatg ccagttttat
     2521 accggagtca tcgatggatt tgataaaaga tgcagatttg ctggtattga atgctttgag
     2581 aaagaaaaaa catccgtctc atttctcttt ggatgaagca ttggaacata ttgaaaagct
     2641 gaggcccaaa gcggcttatc tgacacatat cagtcatttt ttaggtttac acacagaagt
     2701 tcagccaaaa ctgccggcag gcgtttttct tgcatatgat ggtctgactg tggaatgttg
     2761 atattgtgag caaagggttt ttgtgtaatt gaattttcta ttacatttgc aaaaaattca
     2821 ggaatatgaa aaaactatta ctcccgttga ttgtaatcat gttggtattt acgggttgct
     2881 attacgaaga aggccccatt atcagtctgc gtacacccga aaaccggctg gtgaataagt
     2941 ggaaatatga aaagttttca ctgaatggtc aggatcagat gcagcattat caaagcagtt
     3001 ggattcagtt taacagtgat aataccgcta tgttttacga agacacgtca tattcatata
     3061 ccgctgcctg ggaattttcg gatgatttta aaaccttgta tctgaattgt tcagatgatt
     3121 cgctcaatac ctgggaaatt gattacaata ttctcaaatt gcgtgataaa gaattgtgga
     3181 tggaaagtga catggggtca ataacgatgt atattgagct ttctgaatac tgagaaacct
     3241 aacctaaaaa cctatatgaa acatttcgca tctgttatgg caatggcttt tgttgctttc
     3301 atcctgatct cctgcggggg aaaagaaagc aaaacaacgg atcagaccga cactactaaa
     3361 accgaagaaa aagtagcatc tggcaatccg gatcagattt atggtgttga gaaaggcatg
     3421 attgaataca aaatggttac aatgggcatg cccactacca tgacccagta ttgggatgaa
     3481 tacggaaaga aatcttacac acagagttcc atggaaatga tgggtgttac caccatcacc
     3541 catatgctga atgatggaga gtttatttat agttgggatg ctacaacaaa agaaggaacg
     3601 aaggcaaaag tcggcaagtc gcaaaatctg aattacaatg acatgtcgga ggaaatgatg
     3661 gcgaagtatc atatgaccaa agaagggaca gaagaagtga atggcaaaac atgtgatgtt
     3721 tataaaatgg aaatggaagg gaccaagtcc aaaacatggg tatggaaggg aattgccatt
     3781 aaaacagaaa ccatggctat gggaatgact gttaccacag agatgactaa attggaagag
     3841 ggcgctgatg ctcctgaagg tgcatttgaa gtaccggctg gtattaaatt caatgaaatt
     3901 tcggctccag ccagataaag agaatctttt tcgttaagct gcctttggca gcttttttta
     3961 tgccattttg acacattttt gaacagttta atgcttcaaa ctgaccaaat tgaagtattt
     4021 tatgacagaa acccctttgg tattaaagtt gagatgccgc cgacgataaa atttgaaaaa
     4081 tgggaaaaat aataggaatt gacttaggaa ccaccaattc atgtgtggca gtcatggaag
     4141 gcagtgaacc gattgtcatt gccaatagcg aaggaaagcg aacaacaccg agtatggtgg
     4201 cctttaccga caatggtgaa cgtaaggttg gtgatccggc caagcgtcag gccataacca
     4261 atccaacgcg tactgtatat tccatcaagc gttttatggg tgaaaccttt gaccgtgttg
     4321 gaaaagagat tgcacgtgtg ccatacaagg tggaacgagg ccccaataat actccgcggg
     4381 tactgatcga cgatcgtcaa tatacgccgc aggaaatttc ggccatggtg cttcagaaaa
     4441 tgaaaaaaac cgccgaagat tatctcggta tcgaagttac tgaagccgtt attactgttc
     4501 cggcctattt caatgattcc caacgtcagg caaccaaaga agccggtgaa attgccggac
     4561 tcactgtgcg ccgtatcatc aatgagccga cggctgctgc acttgcctat gggctggata
     4621 agaaaaacag agatattaaa atcgccgtat ttgatcttgg tggaggtact tttgatattt
     4681 caatacttga attgggtgac ggtgtttttg aagtaaaatc aaccaatggg gatactcatc
     4741 tgggtggaga tgattttgat caacgcatta ttgaatggtt ggccgaggag tttaaaaagg
     4801 atgaaggtct tgacctccgt aaagatccaa tggcgcttca gcgtttgaaa gaagcagctg
     4861 aaagagcaaa aatcgagctc tcgtcaagtg cgcaaaccga aatcaatctt ccgtatatca
     4921 tgccggttga cggtatgccc aaacaccttg taaagacttt aacacgtgcc agatttgaac
     4981 aacttatcga cgaccttgtt aaagctacca ttgaaccttg tcgcaaggcc ttgtctgatg
     5041 ccaatatgaa aaataccgat attgacgaag tgattctggt cggtggatca acgcgtatcc
     5101 cagttataca gcaggttgtt gaggaatttt ttggcaaaaa accttccaaa ggcgttaatc
     5161 cggatgaagt tgtggctgtt ggtgcgggaa ttcagggtgc agtgctcacc ggcgaagtga
     5221 aagatgtgct tctgctcgat gtaactcctt tgtcgcttgg aattgaaaca cttggtggcg
     5281 taatgaccaa acttattgag tccaatacca ccattcctac caaaaaagct gaaacatttt
     5341 caactgctgc cgatagccag acatcggttg agattcatat acttcagggc gagcgtccca
     5401 tggccaatca aaacaaaagc attggtcgtt tccacctcga tggaattccg cctgcaccac
     5461 gtggtgtccc gcaaatcgaa gttacttttg atatcgatgc caatggcatt ctgaatgtgt
     5521 cggctaagga taaagctacc ggcaaatcgc agcagattcg tattgaagcc agcagtggat
     5581 tgaccgatga tgaaatcaaa aagatgaaag ctgaagccga tgccaatgcc gatgccgata
     5641 aaaaggaacg tgagcgtatc gataaaatca atcaggccga tagcatggtt ttcaataccg
     5701 aaaaacaact aaaagaatac ggagaaaaaa tcccggctga taaaaaatcg gagattgaaa
     5761 cagcgcttac agaactgaaa gaggcacata aggcgcagaa tatcgaagcc attgatgctg
     5821 ctaccgccag attgaatacg gcatggcagg ccgccagcga agaaatgtac aaaaataccg
     5881 ggcctcaggg cggacccgaa gctggtgctg atccgggagc tcagaagcag caacaaaatt
     5941 ccggcagcga tgaagtgacc gatgttgatt ttgaagaagt gaaggacaaa taaacaactg
     6001 tctgaatcta tatcaatatg ccggcattgt gattgaacat gatgccggtt ttttctgtct
     6061 ttttttccta actttgtgtt ttaaagctat agcaatgatt ccttttgctc ctcccagaat
     6121 agatcagaaa attattgatg ccgtcaatga agttcttcga tcaggatgga ttaccacagg
     6181 tccacgtacc agagaatttg aatccatgtt aaaagattat gccggttgtt cagaagtatt
     6241 atgcatcaat agtgcttcgg ccggtcttga actgattttg cgatggttcg gcgttggtcc
     6301 cggtgatgaa gtgattttac ctgcatatac ttatgctgcc acagccaatg ttgttattca
     6361 ctgtggcgca acacctgttt ttagcgacag cagccctgtt gattttaata ttactgttga
     6421 tgaaataaga aaaaatatca ctgcggctac aaaagtgatt gtaccggtcg atttggccgg
     6481 atttccctgt gattattatg caatcaacaa tcttgttgat gatcctgatg tgagtagctt
     6541 gttttcacca gacacagata atcagaagaa actgggtcgg attctggttt tgtccgatgc
     6601 tgcacatagt attggtgcta cttataaagg aaaaagaaca ggcgcattga ccgatatttc
     6661 tgtttattct tttcatgccg taaaaaacct gactactgct gaaggtggtg ctatctgcct
     6721 gaatatgaaa gctccttttg aaaacaatct tatcaaacag gacttgatgc ttaaatcact
     6781 tcatggtcag aataaggatg cgcaggccaa gctgattccc ggaaactgga aatatgatat
     6841 tattgaaccg ggatacaaat gtaatatgac ggatatacag gccgccattg ggatcgttga
     6901 gctgaagcgt tatgacagtg atatgcttgc acgtcgcaga ttgattttca gcatgtatgc
     6961 ccaattgctt caaaaccatc ccagagttca gttgcctgtt tataaaacgg atgtggctga
     7021 gtcgtcatat catgtgtttt tgcttaggat taaaggcatt actgaacagc agcgcgatga
     7081 ccttatacag gaaatattcc gaagagaagt tgctgtaaat gttcatttca cgccgttgcc
     7141 tctgatgact tattataaaa atgccggtta caacatcaat gattatccgg ttgcctatga
     7201 taatttttcg cgagaaattt ctttgccggt ttattatgat ttgagcaacg aacaggtaca
     7261 gactgttgcc aaaacggtta ttgattctat caattcaatc tgtccatgat tggcaaacgt
     7321 ctttttgatt tgtttttctc tttcttttct atcctgattc tacttattcc gggattgatt
     7381 cttgcattga ttatagtgat cgaatcacgt ggaggtgcgt tttaccggca ggtgagggta
     7441 ggacgtaacg aaaaattgtt cagattgatg aaatttcgca ccatgttttc cggtagcgat
     7501 tccaagggct tgctgactgt tggttctact gatagccgcg tgaccggatt tggccggttt
     7561 ttacgtaaat caaaaatgga cgaacttcct caaatgctca atatttttgc agggcatatg
     7621 agttttgtcg ggccacgccc ggaagtgccg aaatatgttg ccatgtatag cgatgagcag
     7681 aaaaacgtgc ttcgtgtcag gccgggactt actgattatg cttcgcttga atacattaat
     7741 gaaagtgatc ttctggcgaa acaatccgag ccggaacgtt tttatataga aacggttatg
     7801 cccgctaaac taagtctcaa tctcaaatac attaaagaga tgagttgtgt gacagatatg
     7861 aaaattattt tccggacact ttttaagatt ggacggagaa ggtaattgtt ctccggcttt
     7921 tgcggatttt cattcgggct tctcctgact ttgacaatgc cttctttata tgcgccatct
     7981 ctgagattac gaggttggaa atatcaatgg cggaagtcag aaaaaaacag agccggacga
     8041 gccggctctg aaataacctg aagtaaaggg ttttatgggt ataaaaacag gttcatgtta
     8101 atgtttcact gtgataagac cctcttgagt ggagccgtca gtgaatttga gaatgaaaaa
     8161 gtaagaaccg tcaggtgtca gtgaggcatc ccagtcgttc tggtagttca gtgatttata
     8221 gatgagcttg ttgctcttgt cgtaaatgct gagttctgta tttggatatt tgctcagatt
     8281 ggtaatggaa aacgtttcgc tgataccgtc gccgttggga gtaatcatat ttgggatacc
     8341 caattcgcaa tgcgtgaata caacaaagaa actgtccaca taggtctggc atcctgtaat
     8401 ctgtgcataa tacaatcctg gcttgctgac agtaagctga ggagtagata gtttatattg
     8461 cggccagtaa tattggtatc cgccggtttt ttgtccaacc gacaaacgca gaattgagcc
     8521 ggtacaggcc gtaatcaggt agtcattgtt cagacttggt ttgggaatga tttttacaga
     8581 aacggcttta gacagttttt tcccgttttc aagtccaatg gttacgcggt aaataccgct
     8641 tttgtcaaca ctgatttcgg gcgttgttgc tccattattc cacagataag tggtttttcc
     8701 ggggaactgg gcgcgtaatg tcattgattg ttccgaacag gtgtcgcgcg gaagcatttc
     8761 aaatgcggaa agtgttcgtt cagcattgtc gctgttttca ttacttaaaa tcggaataag
     8821 atactctgtt tcattgttga tttcaattac ttcagcaagc attttgccat catccggtcc
     8881 ctcattcatg gttgtttgat atgatgctaa ttgcattgca tattcgtcag tcagatgttt
     8941 ttttaatgac tgattgacag gaagttgatt aaaatactgt atcattcttt cagaactgat
     9001 atctgaattg acaacaacaa tttgtttgtt gtcatcttca gctgaaacaa ctgaaatggg
     9061 ttgttcggca ttctcatcta acctgacatc cggaagaatg tagtttttgt ttaccgttga
     9121 agagttgtta ttttgtttgg aagaatccga cgaaaagaaa taaatagccg tagcaacggc
     9181 aatggtagta acaacgccga tagcaacctg aaggggtttg gtgcgtaata gagccagtat
     9241 ggtaacagtt cccactgttt tcattatatc cgacgacaat ccatcgaagt agcccggagg
     9301 tgtcgtaaaa ggattacctt ttcccgaagt ccccaaacct tcgaattcct gatggttgtt
     9361 tttccctgta tgtttacgca gtttcatagt tcgttcattg gactgcaata ttgatgcagc
     9421 gtttaatttg tctttaaaat ttcttcaatt ttttttaccg caatatgata cgatgctttt
     9481 aaagttccga tacttacacc tacaacattg gcaatatcct gatagggcat ggcttcgaaa
     9541 tagcgcatga taaaaaccag tttctgtcgt gctggcaatg ttgccacggc cttctgcagc
     9601 tttcgctgaa tatcggtacc cttgaaatag atatcagatt ctatgcgatc gccaaagtca
     9661 tctctcattt catccatcga aacaaacaga tggctttttt tgctttttag aaacgacagc
     9721 gcctcgttgg tggcaatacg gtatatccag gtataaatct ttgagtcttc gcggaattta
     9781 tcgaggttct gccagacttt gataaaagta ttctgaataa catcgttggc atcgtcgtga
     9841 tcaattacaa tgcggcgaat gagccagtag gcctgctctt tatatttatt cagcaagagt
     9901 tcaaaaccac gctgccgcga tttttcggct ttcagcagat tcaatatttc tgcatcggtg
     9961 attttgctca ttttatttta aaggtttgtt tttctgttta atgctctaat tgctgcatca
    10021 acgatgtttc cggctttaag tccgtatttg accattaatt cctctggtgt tccactttcg
    10081 ccaaatacat cgggcatggc tacatattcc tgagctgtgg gcagtttttg cgaaagaagg
    10141 gccgaaatgg cctcacccaa tccgccggcc tgtacgtgtt cttcggcact gacaacacaa
    10201 cgtgttttcg aaatgctttt cagcaaaagt tcattgtcga gtggtttgat ggtgtgaatg
    10261 tttattattt cagccgaaat gcctttcgtt tccagtatcc gactggcttc cagtgcttcc
    10321 cagaccaaat gtccggtggc cacaatggtt acatcggttc cttcagtaaa aacaattcct
    10381 tttccaatct gaaaaggagt gtcaatgggt gagaagtccg gtaccaccgg ccgtccgaag
    10441 cgaagataca cggggccgtc ccattcagcg atggcttgtg tggccatgta tgtctgcgaa
    10501 taatcacagg tgttgattac ggtcatattc ggcaatgcgc gcatcagggc aatatcttcc
    10561 attatctgat gtgttgctcc gtcttcaccc aatgtgatac ctgcatgcga ggcgcatatt
    10621 tttacatttt tattgctgta ggccacacat tgacgaatct ggtcatatac acgaccggtg
    10681 gaaaaattgg caaaagtgcc tgtataaggt atataacctg caatggcaag acccgaggcc
    10741 attccaatca tattagcttc ggcaatgccg gtctgaaaaa aacgctcagg gaaattggct
    10801 gcaaaggtat tcatcttcag cgatccggtc aaatcggcac aaagagccac tactttttca
    10861 ttttttttcc cgagatctgc caaggcatct ccaaatcctg aacgggtatc ttttttttgt
    10921 cttacttcaa ttggtttcat tcgtttgtct ttattttaga acatcgacat attgactttt
    10981 gtcatggctg ccggttttac tgtaaaattc ttttcgatgg tgagcatgga gcgtgtctgg
    11041 tattttgaac gaaagacagc aaaataatcg cctggttgca acatcagtat ttcttgctct
    11101 ccgcttcctc tgaaattata tacccattcc agcttatttt ctctttttac atacaaactg
    11161 ccatagccgc ttgccggaaa ctgaaaaatg gttgttcctg cagccggaat ttcaacaaag
    11221 gttgttgtac tttggtctat ttttacgtcc tcgacatagg tgcggggtaa tgtcatgatt
    11281 tccagatcat acaatccgca gatatatttt tcgcggttaa agagaaattg agtgttcagt
    11341 gtgttcagtt tgttgttttc ccttacaacg caaagaatcg aattccggct ctgagcataa
    11401 cttccttcgg ttttaacttc cagaaatccc tgcggtgccg aaacacccac aatggtatgt
    11461 ttgcccgctg tcagctcaag cgaatcaata aaaacaggtg gaatggtgtg aacctgtatt
    11521 ctgtaattca gcattggatc gataaccagt gtatcgggaa atcccctgtt gttgagcgaa
    11581 tgtatgaaat tgtattttgg caatccgctc aacttatcat aaaatgtcat gtttacattg
    11641 gtttctgtcg cattgccatt gatgtcgagc agatttacct gacaggtggt tgaattcagt
    11701 gcttgcgaaa taacgatatt caaggcagta ctgaattgtt tttcggtggt ggcatcgaaa
    11761 taggtgccaa cacaatcaaa ctgttccttg aaacttttcc cgattccgat gatgaatggt
    11821 ttcaatacaa tccctttttt ctgcaaagca agtgagacag cacagggatc gccgttacat
    11881 tcttcgagcc cgtcggtaat cagaataata atgttgcggc agttggcgca gggtggaaaa
    11941 tcacctccgg ctttttcgag cgagtaggca ataggggtcg ttccccgcgg attgatggtt
    12001 ttaagtttgt atttaatctt ttctatgtta tcgggcccga agggcacttc taagcgggta
    12061 tcatcacagt cttgcggtgg atagtccttt gtatggccat aacagcgtaa agcgagttcc
    12121 agattgggta catcgcgcag actgtcaagc agatcggaca tgatttttct ggcaatggta
    12181 attttcatgt cgctttgcca gcgtccatac atagattgac tggcatcgaa cagaaaaaga
    12241 atacgcgtgg tgattacttt ttccttgtca ggagtgcctg tttgagctgt tgcaccaaaa
    12301 gccagcagta aaaaaacaag tatggcggat agttttctca ttttcagtag tcgcccagcg
    12361 tttcagtaag ttcgcttagt gccttatttt tttgttcatc actgggtgga gagccgtgcc
    12421 atttgtgact tccttccata aacgaaaccc cttttcccat gatggtggtc atgagaatta
    12481 caaccggttt gcctttgcca ctcagtgcct tggcattttc catggtgttc aggatttgtt
    12541 gcatgttgtg tccatccatt tcaagcacag tccatccgaa ggatgtccac ttggccgcca
    12601 aatcgcccaa tgaaacaact tcgtcaacag gaccatcaat ttgttttcca ttgtaatcaa
    12661 tggtggcaat gagattgtca acttttttgg cagcagcaaa catagctgct tcccagattt
    12721 ggccttcctg cagttctccg tcgccatgaa gggtataaac aaaagttttt tcgccggata
    12781 attttttgcc gagtgcatta ccgattgcag cagagagtcc ttgtccgagc gatccggatg
    12841 caatgcgcac tccgggcaga tgttcggccg gtgtagggtg accctgcagc cgggagccca
    12901 gttttcggaa tgtcccaagt tcactgatgg ggaaatatcc ggatcgtgcc agcacactgt
    12961 accaggccgg acttatatgc ccgtttgaca gaaaaaatac atcttcgttg gttccctgca
    13021 tggaaaaaga tgccgggtta tgattcatca cattgttgta caatgcaaca aggaaatcag
    13081 tgcatcccag cgacccgccc ggatgtccgg attgaacggc atgcaccatg cggatgatgt
    13141 cgcggcgcac ctgagatgaa aatttgttca gttcttcgac agaaagcatt tttttaggtt
    13201 caaagataac tgaatattca attcgttgtg aaaaaaaatg tccacatgtt tttgtgaata
    13261 acaatatctc aaatttgcag tgaattagac tttttaatag tttttcatta tatttgcaat
    13321 taaaatatcc atcatatgaa atatctcatg gtttgtctgg gcaacatttg ccgtactcct
    13381 attgcagttg ggctgctgcg ca
//
LOCUS       DAOPKE010000074        13355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4854_length_13355_cov_12.431203, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000074 DAOPKE010000000
VERSION     DAOPKE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4854_length_13355_cov_12.431203"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..673)
                     /locus_tag="PKY63_09425"
     CDS             complement(2..673)
                     /locus_tag="PKY63_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF00874.1"
                     /translation="MIIEKYNIRLRSLNEEDLEMVRKARNSDYIRSRMIYQKIISPEE
                     QMHWYHSLNPATDIFMIIEYDEKPRGMINVKNIDYQNDLSESGVFFWDKEVLQSFVPV
                     MTSWLAAESGYLLLGGQQTLIHVLKNNKTAIDFNRKQGYEITKEMENIILMRQTRKSF
                     SQATLSERTKFLQTIKKSSQLKLCFGDHTQDDFRMNHLLNYLKILGVSAIKQSGQTFW
                     FEIDF"
     gene            complement(670..1593)
                     /locus_tag="PKY63_09430"
     CDS             complement(670..1593)
                     /locus_tag="PKY63_09430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF00875.1"
                     /translation="MMENSKISFVIPVYNNPDCLEEIVKLALKAISDEHCLAEIIFIN
                     DGSTDESWNSICQLAEKHKGIVYGLDLMRNYGQHHATLCGIQRATGDFIITLDDDLTH
                     SPQQAMQLLHFMLSENLDLAYGISAKRKSGIGRSIGRYMLYLSSSVGSKRIAGASFRA
                     MTATLAKEMNPEGDVIFIDDILTSITDRYHYKTFENFRNNKQSRYSNKLLLKMGINIS
                     LFYSGFPLRLLSMIGFAGSLISGVFGMFYLYKKLFFKVPMGYTSIIVAVLFSASAMLL
                     GMGILGKYLYRIHGNRNRVLPYHVRKTTKDA"
     gene            complement(1590..2324)
                     /locus_tag="PKY63_09435"
     CDS             complement(1590..2324)
                     /locus_tag="PKY63_09435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF00876.1"
                     /translation="MNKLKTILITGATSGIGCRIAENLNGKYQLFLSGRNETRLSEIN
                     KKLSHVLPYVTCDVSFETDVEKLASEAPQIDGLVYCGGIAPTIPVKYIRENDILKTFA
                     VNFNGAVLVVAALLKQKKINPGSSLIFISSEAVRHPFFGSTVYSASKAALESFALTLS
                     NELQSKKIRANCISPTYVESPMLDQARNTMSGDFIAGMKKMHPEAFNTADHIASMAAF
                     LLSDESAAINGQIIKAGRFNINIPGL"
     gene            complement(2317..3378)
                     /locus_tag="PKY63_09440"
     CDS             complement(2317..3378)
                     /locus_tag="PKY63_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPF00877.1"
                     /translation="MADVKINNIRIAGIAACVPENVDHIADYTLISESERAGFMKTVG
                     VHERRMVSKGVVTSDLCAQAAVHLMKKLEWDPSTINALVFVTQTSDYVIPSTSIILQH
                     RLGLPESCMAFDMNLGCSGYVVGLQVASSLINPTGIRRVIFLAGDVSSINMSYHDKST
                     YPLFGDAGSATAIEYCENAPEAIFSTGSDGKQFDALYVKAGGTRHMATAESFEYKEYP
                     GGIKRNDTQMILDGLRVFNFSITNVPPQIENILRQASASVETTDMFFLHQANRIMVET
                     IRKKLRIEPEKCPYSIDLFGNTSSASIPVTMCHSIGNSSIQQSLNVVLSGFGIGLSWA
                     SAWVSLKNALILPIVEYHE"
     gene            complement(3371..3616)
                     /locus_tag="PKY63_09445"
     CDS             complement(3371..3616)
                     /locus_tag="PKY63_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPF00878.1"
                     /translation="MELLISDFISRIESEFDDVVPGSVKPETKFRDHFDWTSVNALVL
                     FSMINVEYGVTLTAEDLQKSTTIQDLFNIISLKIANG"
     gene            complement(3662..5137)
                     /locus_tag="PKY63_09450"
     CDS             complement(3662..5137)
                     /locus_tag="PKY63_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF00879.1"
                     /translation="MSKIPLHAGLVADFIAIEFADIPQVATENTLFLKKVLRITSWLR
                     LQGVKKNSLIVSLFPPDKNWIALDMAILRCGAIHVPFHMADEISLAAEYFEQYLFIVH
                     RDIKYQTDTKANQIIVTDLSDDFFQDSDIHNSTDNKAVEVDPHHPAEIIFSYHRDCGP
                     KPFVISHDNLLKTAVAAGKELPLTPGDKYLSLLPFSKIYGRVSLLTHQLLGCIIECPQ
                     QILLPSRLIKVSKAASVAVVPSMLQYPVKVDGACSQLLKGRSLATLDELPPEAIRPVL
                     GDTLKIIICGGAMIPSDLLQKFEAASLPLMEGYGLTQTTGVFTLNTVSAHRTGSKGKA
                     LNHMSIKISDDGEILAKGPGISSGIILPDGTIEKIDDSNNWFHTGDEGYIDADGFLFL
                     TGNKKNVFKLANGYYYNPQPDEKRIADEINALSMICRDEKGNLHLLAEKITLSSEELE
                     FLKKFRTNPFSMPLTSLFQTKTLPCCRPYVVVANENWISLK"
     gene            complement(5134..7086)
                     /locus_tag="PKY63_09455"
     CDS             complement(5134..7086)
                     /locus_tag="PKY63_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00880.1"
                     /translation="MSIKTQYLVIALLSALLYANTIGNRYALDDDYVICTSENVQQGF
                     FKTVSDIAITHYSITEKSRFEYRPVVKITFAAEYFLWGNNPHLSHAINVLLFVITVLL
                     LFRLLHRLFGLRYPWLPFLTAIIFAAHPIHTEVVASLKNRDELLAFLFSIWTAWLVLK
                     YCDTKKWYFLALSFPVFLIAYFSKSSALVFVALIPLIMWFAGIKNLKYYLTGFLLIAA
                     AAWIARWAPSQFLAESHRAVYGFENPIYEHRGIFYRLGTGLVALLFYARILIIPYPLR
                     FYYGFDTIPLTHFPDLISIISLIFHIGLLFVAILYFRKKHPLSFAILFYLIAISMFSN
                     IVKPAVGIVAERYVYAASLGFCLTVAWFILKLFRYENKPVVSWININKGIRLIMLGLV
                     FIYGARTISRNSVWKDHLTLYYNDIKHCENSFKANFLLASTLHAEVNKTINDPRLGKR
                     NARYITDAEHYYNTAIGIYDRYPVIWNSRGFFRFMFKKENPQAIHDFKKALALNPNYT
                     EALYNLGFVYHQQKNYDSAIVYFERAIVSNPDYIKAYNEKGLAMLNSNDTAGFLTIQT
                     SIKSKFPESDIPYINLGNYFLGLKDTTRAFENWEMAAKRNSDNQALLLNLSNYYARQG
                     NQEKSQHYITLYRQAVQKQKNLKRKQ"
     gene            7230..7679
                     /locus_tag="PKY63_09460"
     CDS             7230..7679
                     /locus_tag="PKY63_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPF00881.1"
                     /translation="MKKFFALFVLVFFAGLFLNAQIVSYTVKTSAKCTNLTAGFTVPA
                     GKVANIKSLDVVPTFKSCNTAAPDLSSIVVSVRSTPLTSSTNRASETSSLKNKGVVVY
                     KKTLEKTGLVTESVDATTVKLLEGTYVIEVSPSAGTEATLTVELFDN"
     gene            7699..8115
                     /locus_tag="PKY63_09465"
     CDS             7699..8115
                     /locus_tag="PKY63_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00882.1"
                     /translation="MKKIFSAVLMVFVFGALVLNAQNFKYSVKASADCTNLKVEFTVP
                     TGKVAKLMSLDIVTSFATCNVSQPAPTYNYAVVYNKIGKTEKTKQSFLYKKTVTSAGA
                     VTESVPIQDVKLNPGVYVLEISRAPNLEATLEVMIK"
     gene            complement(8162..8782)
                     /locus_tag="PKY63_09470"
     CDS             complement(8162..8782)
                     /locus_tag="PKY63_09470"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPF00883.1"
                     /translation="MLLEIHPVNPQEKYIHQIVESLNQGGVVVFPTDTVYAIGCSLYQ
                     HKAVERIARIKGIRTDKAKFSILTDGMSNLSEFTKPINNHIFRLMKKVLPGPYTFILP
                     ASSKVPGIFESKKKTIGFRVPDNLILQMIISKLGHPLVSTSLHSDDDDYLEYVVNAEL
                     IYEKYENLVDYVIDGGSGGIEPSTVLDCTSDDIEIIREGKGAIDML"
     gene            complement(8783..9439)
                     /gene="fsa"
                     /locus_tag="PKY63_09475"
     CDS             complement(8783..9439)
                     /gene="fsa"
                     /locus_tag="PKY63_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPF00884.1"
                     /translation="MKFFIDTANLKEIETARNWGILDGVTTNPSLMAKEGIKGQNNIL
                     KHYVDICNIVDGDVSAEVIALDFDGMMREAEILASLHKNIVVKVPMTKDGIKAVKHLS
                     GEGIKTNCTLVFSAGQSILAAKAGATYVSPFVGRLDDISADGMALIAQMVNIFSMQEY
                     ETQILAASLRHPMHIVQCAEYGANVVTCPFKTIEQLFHHPLTEIGLAKFMEDYKNSLK
                     "
     gene            complement(9574..9672)
                     /gene="ffs"
                     /locus_tag="PKY63_09480"
     ncRNA           complement(9574..9672)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKY63_09480"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            9768..10850
                     /locus_tag="PKY63_09485"
     CDS             9768..10850
                     /locus_tag="PKY63_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00885.1"
                     /translation="MKILSSICFGILFFCMINVVAQTTFTAYDRSSATAKDAWDQYYK
                     NGTAPDVAAFDEKNPFDRSLKFLVAASMHIVADSTVKWTVASVLACRDHLDTLPFIIP
                     VELQQRLSVNCKNGREQLLDGLMQLYYLAPWQCAYFIKEKEALCRQNSNEKILAFSLL
                     NRALLYSEVLGKPERALPLIDSAIVIWTKLGDHSQLANNLKARGIVFLRMKKFREAER
                     EIKSAMALYKKSDTQYGISSCQLDLVLIFAQSGEPDSMKKYDQLCRPGFEALDTMRLF
                     VLNTNRLAAYQTANQYSIRDIAKQNAGLLFSSQFHPAMVIDYYKYSMKAFTFFGETAL
                     LKKYNSEYANMQQELLKQGYWPGLFK"
     gene            11333..11614
                     /locus_tag="PKY63_09490"
     CDS             11333..11614
                     /locus_tag="PKY63_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00886.1"
                     /translation="MNNQLIILIVLFVYLVISAYSVLWISRNKLLERAQKIRNICITL
                     LLPFWIIIVRSALKPFKYKETNDRGWIRQEHEGSVRGTWIGGMFNQSGK"
     gene            11752..12093
                     /locus_tag="PKY63_09495"
     CDS             11752..12093
                     /locus_tag="PKY63_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HPF00887.1"
                     /translation="MSSIKLFDEMQIRVVWSTTKQKWYFALSDVVIALTNTVNVKQYI
                     KYMKKRDKELAQNWYQFVTLMPVETPGGLQNMKCASKDQLLRIIQLVRSSKKQKFKIW
                     LAKVSSQKKNK"
     gene            12090..12572
                     /locus_tag="PKY63_09500"
     CDS             12090..12572
                     /locus_tag="PKY63_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00888.1"
                     /translation="MIPDRTETQFITAPMRSLKQHAVELNSCVFYGKWHWFWGYWDAF
                     SWVYGLSEEEIRDFMNRYQHARDNIQNIISVMENGRLKDKLTSEYRFFPESNAGQYVN
                     SHWYLWLFFILSPIKKIRKTHQFKRFLDDIASTSERMINYTENAAWLEMVPEKEDHGL
                     "
     gene            12676..12924
                     /locus_tag="PKY63_09505"
     CDS             12676..12924
                     /locus_tag="PKY63_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HPF00889.1"
                     /translation="MGKNTSILLGDYFEKYVNEQLRTGKFATVSEVIRAALRLMESEE
                     NKSKTLVKELKAGERSRKVKNFDRAENLKKLHAENTVR"
     gene            12921..13217
                     /locus_tag="PKY63_09510"
     CDS             12921..13217
                     /locus_tag="PKY63_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF00890.1"
                     /translation="MKYEISEKAIEDINQIWLYTFENWPQEQTDRYYNQIFDEIEFIA
                     ANFEDGRSMEHIRKGYRAGKVKSHLIFYRKSKKNVVEIIRVLHQHMDIENLMSE"
BASE COUNT         3933 a         2585 c         2900 g         3937 t
ORIGIN      
        1 ctcagaaatc aatttcgaac caaaacgttt gtcccgactg ctttatagct gaaacgccca
       61 gtattttcaa ataattcagc agatgattca tccgaaaatc atcttgtgta tggtcgccga
      121 aacatagttt caattgcgaa ctttttttga ttgtctgcaa aaattttgtt cgttccgata
      181 atgtggcctg tgaaaatgat tttctggttt gacgcatcag aataatattt tccatttcct
      241 ttgttatttc atatccctgc ttgcgattga agtcaatggc tgttttgttg ttttttaaaa
      301 catgaatcaa cgtttgttga ccacccagca gcagatatcc tgattcggct gccagccacg
      361 aagtcatgac cggaacaaaa ctttgcaaaa cttctttgtc ccaaaaaaac acacctgact
      421 ctgataaatc attttgatag tcgatatttt ttacgttaat cattccgcgc ggtttctcat
      481 catattcaat aatcataaaa atgtcagtag ccggattgag cgaatgatac caatgcattt
      541 gctcttcggg tgaaataatt ttctgatata tcatccggct acgaatatag tcgctgttgc
      601 gagctttacg caccatttca aggtcttctt cattcagcga tctgagtcgt atgttgtatt
      661 tttcaataat catgcatcct tagtagtttt gcgcacatga tagggcaaaa cacggtttct
      721 gtttccgtgg atacggtata gatatttgcc cagaatgccc atacccagca gcatggccga
      781 tgcagaaaac aacaccgcaa caataatgga tgtgtaaccc atcgggactt tgaaaaacag
      841 ttttttatac agataaaaca tgccaaaaac tccgctgata agcgatccgg caaatccgat
      901 catagaaagc aggcgcaatg gaaatcctga atagaacaac gaaatattga tccccatttt
      961 cagcagcaat ttgttgctgt agcggctttg cttattattt ctgaagtttt caaaagtttt
     1021 gtaatgatat cggtcagtta tcgatgtgag tatgtcatca ataaaaatca catcgccctc
     1081 cggattcatt tcttttgcca atgtagctgt cattgcacga aacgatgcac cggcaatgcg
     1141 tttgcttcct actgaagagc tcagataaag catatatcgt ccgatggaac gtccgatacc
     1201 cgattttctt ttagcagaaa ttccataagc caaatcaagg ttttcgctga gcataaagtg
     1261 cagaagttgc attgcctgct gcggtgaatg tgtcaggtcg tcatcgagtg tgatgataaa
     1321 atcccctgtg gcccgctgaa ttccgcaaag tgttgcgtgg tgctgtccat agttgcgcat
     1381 caggtcaaga ccataaacaa ttcctttgtg cttttcagcc agctggcaga ttgaattcca
     1441 tgactcatcg gtgctgccgt cgttgataaa gattatttct gccagacagt gttcatcaga
     1501 aatcgctttc agtgcgagtt tgactatttc ctcaaggcag tccggattgt tataaactgg
     1561 aattacaaat gaaattttgc tgttttccat cataggccgg gaatattgat attgaatctg
     1621 cctgctttga ttatttgtcc gttgatggct gctgattcat cgctgagcag aaaagcagcc
     1681 attgatgcaa tatggtctgc tgtattgaat gcttccggat gcattttttt catgccggca
     1741 ataaaatcac ccgacattgt attccttgcc tgatcaagca tgggtgattc aacgtaggtt
     1801 ggcgaaatgc agtttgctcg tatttttttt gattgcagtt cattcgacag ggtaagtgca
     1861 aatgattcaa gtgctgcttt tgaagccgaa tacacggtgc ttccgaaaaa cggatgccga
     1921 acggcttcgc tgctgatgaa aatgagtgag ctccccggat ttattttttt ttgcttcagc
     1981 aatgcggcta caaccagtac ggcgccattg aaattgactg cgaaggtttt cagaatgtcg
     2041 ttttcgcgaa tgtattttac cggaattgtt ggtgcgatgc caccgcaata caccaatccg
     2101 tcgatttgtg gtgcttctga agccagcttt tcgacatcgg tttcgaagga tacatcacaa
     2161 gtgacataag ggagcacgtg gctgagtttt ttattgattt cagataatct tgtctcgttg
     2221 cggccgctta gaaataattg atatttccca ttcagatttt ctgcaatccg gcaaccaatg
     2281 cccgatgtgg cgcctgtaat caatattgtt tttagtttat tcatgatatt cgacaatcgg
     2341 taaaatgagt gcatttttta aactgaccca tgctgaagcc caggataaac caatgccgaa
     2401 gccactcaga acaacattga gtgattgctg aatcgagctg ttcccaatgg aatgacacat
     2461 ggtaacagga atgctggcag agctggtgtt accaaacaaa tcaatggagt aagggcattt
     2521 ttcgggctca attctgagtt ttttccggat ggtttcaacc atgatgcggt tggcctgatg
     2581 aagaaaaaac atgtctgttg tttcgacaga tgccgatgct tgtcgcagga tattttcaat
     2641 ctgtggagga acatttgtaa tgctgaaatt aaaaacacgc aggccatcaa gtatcatttg
     2701 tgtgtcgttg cgtttgattc cgccaggata ttctttatat tcaaaacttt cagctgtggc
     2761 catgtggcgc gttccgcctg cttttacata taatgcatca aactgtttcc cgtcgcttcc
     2821 ggtgctgaaa atggcctctg gagcgttttc gcaatattct atggctgtag ccgatccggc
     2881 atcaccaaac agcggatatg tgcttttatc atgatacgac atattaatgg aagaaacgtc
     2941 accagccaga aaaatcacac gacggatacc tgttggattt atcagtgatg aagccacctg
     3001 cagaccgacc acatatcccg aacaacccag attcatatcg aaggccatac aactttcggg
     3061 caatccgagc cggtgttgca gtatgattga tgtacttgga attacataat ccgatgtttg
     3121 ggtaacaaat accaatgcgt ttatggttga tggatcccat tctagttttt tcatcagatg
     3181 aactgccgct tgagcacaga gatccgatgt taccacgcct ttgctcacca ttctgcgttc
     3241 atgcacccct acggttttca taaaaccggc gcgttcgctt tcgctgatga gtgtataatc
     3301 ggcaatgtga tctacatttt ccggaacaca tgcggctatg ccggctatgc ggatattatt
     3361 gatttttaca tcagccattg gctattttga gagatatgat attaaataaa tcctgaattg
     3421 tagtcgattt ctgaaggtct tcagcagtga gtgttacacc atattccaca ttgatcatgg
     3481 aaaaaagtac cagtgcattc accgatgtcc agtcaaaatg atccctgaat tttgtttcgg
     3541 gctttacaga acccggaaca acatcatcaa attcggattc aatcctcgaa ataaagtcgc
     3601 ttatgagtaa ttccatggca gatacggtaa aaaacaaaga tataaaaaaa atcagactga
     3661 tttatttcag agaaatccag ttttcatttg caacgactac ataaggtctg cagcaaggca
     3721 gcgttttcgt ctgaaatagc gatgtcaatg gcattgaaaa tggatttgtc ctgaactttt
     3781 ttaaaaattc aagttcttca gaactcagcg ttatcttttc tgccaacagg tgaagatttc
     3841 ctttttcatc cctgcaaatc atcgacaggg cattgatttc atctgcaatt cttttttcat
     3901 ccggctgtgg attgtaatag tatccgttgg ccagtttgaa aacgttcttt ttatttcctg
     3961 tcagaaataa aaatccatca gcatcaatgt agccctcatc gccggtgtga aaccaattgt
     4021 tgctgtcgtc aattttttcg atggtcccgt ctggtaatat tattccggag ctgattcctg
     4081 gcccttttgc aagaatttca ccgtcgtctg aaatttttat tgacatatgg ttaagggctt
     4141 ttcctttgct gccggtgcga tgtgcactta cagtattcag cgtaaacacg cccgtggtct
     4201 gtgtgagtcc gtagccctcc atcagaggaa gagaagccgc ttcgaatttt tgcagcaaat
     4261 cagacggaat cattgcgcca ccacaaatga ttattttaag agtgtcgccg agcacaggtc
     4321 tgattgcttc aggaggaagt tcatccagtg tcgccaggga tctgcctttc agcaattgag
     4381 aacaagctcc atccactttt acgggatact gaagcatgga aggaacaacc gctaccgatg
     4441 cagctttcga aacttttatc agccgcgatg gtaataatat ttgttgtgga cattcaatga
     4501 tgcagcccag caactgatgt gtcagtaaag aaacacgtcc gtaaattttt gaaaacggaa
     4561 gtaaagacaa atatttgtca ccaggggtca atggtagttc ttttcctgca gcaacagctg
     4621 ttttcaatag attatcatga ctgattacaa atggttttgg tccgcaatca cgatgatatg
     4681 aaaaaatgat ttcagccggg tgatgtggat cgacttctac ggccttgtta tctgtagaat
     4741 tgtgaatatc ggaatcttga aaaaaatcat ccgataaatc ggtgacaatt atttgatttg
     4801 cttttgtgtc ggtttggtat tttatatcgc gatgaacaat aaacagatac tgttcgaaat
     4861 attctgctgc caatgaaatt tcatcggcca tatgaaacgg aacatgaatg gcgccacatc
     4921 gcagaatagc catatcaaga gctatccagt ttttatctgg cggaaaaaga gaaacaatca
     4981 gcgaattttt ttttacacct tgcagtcgga gccatgaagt aatgcgcaaa acttttttga
     5041 gaaataatgt attttctgtt gcaacctggg gaatgtcagc aaattcgatt gctataaaat
     5101 cggcaaccag acctgcatgc agaggtattt tgctcattgt ttccgtttca gatttttttg
     5161 tttttgaaca gcttgccgat acaaagttat gtaatgttgc gatttttcct gattgccttg
     5221 ccgggcataa taattcgaca ggtttagtaa aagggcctga ttgtcggaat tgcgttttgc
     5281 agccatttcc cagttttcaa atgctcgtgt tgtgtcttta agaccaagaa aataattgcc
     5341 aagattaatg tagggaatat cggattccgg gaatttgctt ttgatgcttg tttgtattgt
     5401 taaaaatccg gcagtgtcat ttgagttcag catggccagc cctttttcgt tgtatgcctt
     5461 aatataatcc ggatttgaaa cgatggctct ttcaaaatac acgatggccg aatcgtagtt
     5521 tttttgttgg tggtaaacaa aaccaagatt gtacaatgct tcggtgtagt tgggatttaa
     5581 tgccagagct tttttgaaat catggattgc ttgcgggttc tcttttttaa acatgaaacg
     5641 gaaaaatcca cggctgttcc atattaccgg atagcggtcg tatattccga tggcggtatt
     5701 ataataatgt tcggcatctg taatatagcg tgcattgcgt tttccgagtc tggggtcatt
     5761 gatggttttg tttacttcgg cgtgcagggt ggatgcgagt agaaaattgg ccttaaagct
     5821 gttttcacag tgttttatgt cgttataata aagtgtcagg tggtctttcc aaacactgtt
     5881 ccggcttatg gttcgggctc cgtaaataaa taccagtcca agcatgatca gtctgattcc
     5941 tttgttgatg ttgatccatg atacaaccgg tttgttttcg tatctgaaaa gtttcagaat
     6001 aaaccatgcc acagtcagac agaaacctag cgaagcagca tagacatacc tttcggcaac
     6061 gattcctacc gcaggtttca caatattcga aaacattgag atggctatca gataaaacaa
     6121 aatggcaaac gatagaggat gtttttttct gaaataaaga atggcaacaa acaaaagtcc
     6181 gatatgaaaa atcaatgaaa tgattgatat cagatcagga aagtgggtga gcggaatagt
     6241 gtcgaaacca tagtagaatc gcagtggata agggataata agaattcgcg cataaaagag
     6301 cagcgctaca aggccggttc ccagccggta aaagattccg cgatgctcgt aaatgggatt
     6361 ttcaaatcca taaacagccc tgtgtgattc ggcaagaaac tgcgagggag cccagcgcgc
     6421 aatccatgct gctgcagcaa tgagtaaaaa gccggtcaga taatatttta aattttttat
     6481 gccggcaaac cacattatca gcggaatcag agcgacaaaa acaagggcgc ttgatttcga
     6541 aaaataggca atcagaaaaa cgggaaatga cagtgccaga aaataccatt tttttgtatc
     6601 acaatatttc aataccagcc aggccgtcca aatactgaaa aggaatgcca gcagttcatc
     6661 gcggtttttc agactggcca ccacttcggt gtgaatcgga tgtgctgcaa aaataatggc
     6721 tgtgagaaac ggaagccagg ggtatctgag gccaaacagc cggtgaagaa gtctgaacag
     6781 gagtaaaact gtaatgacaa acagcagcac gttgatggca tggctgaggt gcggattgtt
     6841 tccccagaga aagtattcgg ctgcaaatgt tatttttaca acaggccggt attcaaatct
     6901 cgacttttct gtaatggaat aatgcgttat ggcgatgtcg gatacagttt taaaaaatcc
     6961 ctgctgaaca ttttcacttg tacagattac atagtcatca tcaagagcgt atcggttgcc
     7021 tatggtgttt gcataaagca aggcactcag caaagcaatg acaaggtatt gggttttgat
     7081 actcatagcg caagattacg gcgaagttag tttttttttg gataagtagc ggtttttata
     7141 ttttttgtat ttttgttttc aagagattgc cggaattttt ctggcacagt tttttataat
     7201 ggcggcaaat ttttaaaaca ttaaaaatca tgaaaaaatt ctttgcactg tttgtgttgg
     7261 tatttttcgc aggtctgttt ttaaacgctc agattgtttc atatactgtg aaaacttcgg
     7321 ccaaatgcac taatctgaca gccggtttta ctgtccctgc cggtaaggtt gccaacatca
     7381 aaagtctcga tgttgttcca acctttaaaa gttgcaatac cgcagcgcct gatttgagtt
     7441 ccattgttgt ttcagtaaga agcacccctt tgacaagttc aaccaacaga gcttcagaaa
     7501 cttcatcatt gaaaaacaaa ggtgttgttg tttataagaa aacacttgaa aaaacaggtc
     7561 ttgtaactga gtctgttgat gctacaacgg tgaaacttct cgaaggcacc tacgttatag
     7621 aagtgtctcc cagtgcagga accgaagcga cattaaccgt agagcttttt gataattaaa
     7681 atataaaatc aaaaccccat gaaaaaaatc ttttctgctg tattaatggt atttgttttt
     7741 ggtgcacttg ttttaaatgc acagaatttc aaatactcag tcaaggcatc tgctgactgc
     7801 accaatctga aggtagaatt cactgttccg acaggcaaag tagcaaaact gatgagtctg
     7861 gacattgtta cgagttttgc aacatgcaat gtgtcacaac ctgcacctac atacaattat
     7921 gccgttgtgt acaataagat tggaaaaact gaaaaaacca aacagtcttt tttgtataaa
     7981 aaaaccgtta cctcagccgg tgctgttacc gaaagcgttc ctattcagga cgtaaaactg
     8041 aatccaggcg tttacgtgtt ggaaatcagc agagctccaa atcttgaagc gacattggaa
     8101 gtgatgatta agtaatgtat ttcgaaatgg gaagaaccgg ctccggccgg tttttttgtg
     8161 ctcagagcat atcaatggca cctttgcctt cgcggattat ttcaatatca tcgctggtac
     8221 agtcgagtac tgtggatggt tcaatgccgc cggagccgcc atcaatcaca taatcgacca
     8281 gattttcgta tttttcataa atcagctcgg cattgacaac atattccaga tagtcgtcat
     8341 cgtcgctgtg aagcgaggtt gagaccagcg gatgccctag ttttgagatg atcatttgta
     8401 aaatcagatt atcaggaaca cgaaagccaa tggttttttt cttggattca aatattccgg
     8461 ggactttgct gctggccggg agaatgaatg tatatggccc gggcagcact tttttcatta
     8521 aacggaaaat atggttgttg atgggtttgg tgaattcgct cagattgctc ataccatctg
     8581 tcaggataga aaatttagct ttatctgttc tgatgccttt gattcttgca atcctttcta
     8641 ctgccttgtg ttgatagaga ctgcagccga tggcatatac agtgtcggta ggaaaaacca
     8701 caactccgcc ctgattcagg ctttcgacaa tttgatggat atatttctcc tgcggattga
     8761 caggatgaat ttcgagtaac atttatttca gtgagttttt ataatcttcc ataaattttg
     8821 ccagacctat ttcggtaagc ggatgatgga ataattgttc aatggttttg aaagggcagg
     8881 tgactacatt ggcaccatat tctgcacatt gcacaatatg catcggatgg cgcaaactgg
     8941 cggcaagaat ctgtgtttcg tattcctgca tgctgaaaat attgaccatt tgggctatca
     9001 gagccatgcc gtcggccgaa atgtcatcga ggcgcccaac aaaaggcgat acatatgtgg
     9061 cgcccgcttt ggcagccaga atcgattgtc cggcactgaa caccaatgta caatttgttt
     9121 tgattccttc acctgaaagg tgcttcaccg ctttgattcc gtctttggtc atcggaactt
     9181 ttaccactat atttttatgc agtgatgcga gaatttcagc ttcgcgcatc attccgtcga
     9241 agtcaagcgc aatcacttcg gcgcttacgt cgccatcgac aatattgcag atatcgacat
     9301 aatgtttcag aatattgttc tgccctttga tgccttcttt ggccatcaga ctgggattag
     9361 tggtcacgcc atcgaggatg ccccagttcc gggcggtttc gatttctttt aaattcgcag
     9421 tgtcaatgaa gaacttcatg attcaatttg aaaattcggt gatttgaaaa tttgaaaatt
     9481 aaaaggatac ttttacctgt cacccttaat ttctttcatc ttttgccttt tatcttttat
     9541 cttttagtac ttgcttggtt gctaaaaata aaaaatgggt aagttacctg catcacaccg
     9601 ctacaaccac ttacccttgc ttccttccga acctggggga gttcgcggga gctggttgta
     9661 caggacttac ccactgcaaa agtaatcatt ccttttgaaa caatggtgtt ttcctgaaaa
     9721 aaatgatgtt tatttgcata aaatattttc tgtcaaaaaa ttatccgatg aaaattctgt
     9781 catcaatttg ttttggaata ttgttttttt gcatgataaa tgttgttgca caaacgactt
     9841 ttacggctta tgaccgcagt tcagccacag caaaagatgc atgggatcag tattacaaaa
     9901 atggtactgc acctgatgtt gctgcatttg atgaaaaaaa tccatttgat cgttcattga
     9961 aatttctggt ggctgccagc atgcatattg ttgccgattc taccgttaaa tggactgttg
    10021 cgtcggtgct ggcctgccgt gatcatctcg acacacttcc gtttatcatt ccggttgaat
    10081 tacagcaaag attatcggtc aattgcaaaa atggccgcga gcagctactc gatggattga
    10141 tgcaattgta ttatctggct ccctggcaat gtgcttattt tattaaagaa aaagaagcat
    10201 tatgccggca gaactcaaat gaaaaaatat tggcattctc tttactgaac cgggctcttt
    10261 tgtattccga agtgcttggt aagcccgaac gcgcattgcc actgattgat tcggccattg
    10321 tcatctggac aaagttgggt gatcattcac aactggccaa caatctcaag gcacgtggta
    10381 ttgtatttct gagaatgaaa aaattcaggg aagctgaaag agaaattaaa tccgcgatgg
    10441 cgttgtataa aaagagcgac actcagtatg ggatatcctc atgtcagctc gatctggtgt
    10501 tgattttcgc acagtcaggc gaaccggatt cgatgaagaa atacgatcag ttgtgccggc
    10561 cgggatttga agcgctggac actatgcggt tgtttgtgct gaacaccaat cgcctggctg
    10621 catatcagac agccaatcaa tacagcatcc gcgacattgc gaagcagaat gcgggcttgc
    10681 ttttcagctc acagtttcat ccggccatgg tgattgatta ttataagtat tccatgaagg
    10741 catttacgtt ttttggcgaa acggcgctgc tgaaaaaata caattcagaa tacgcaaaca
    10801 tgcagcagga gcttttgaaa cagggatact ggccggggtt gtttaaataa ggcgtaagtc
    10861 gataggcata aagatcaata catcttgagt tgaaagttcg gaaagtttaa agttataaag
    10921 gcgatgtaaa agcacagggc atggagcaag gagcatgcaa agcttcgcgc tcgcgctgta
    10981 tttgacagat ggagtacaac gcacaatgcg ttatttaaat gtgccgaagg caaaaacaaa
    11041 gctacgcagt acctatgaag gggctgcagc gcagcccaac aataatagac ttgggcagaa
    11101 agccccaggt tcaaggtgtg cgacctgtca cgtattacgt gaagcacctt tgagttgata
    11161 ggtcatgcgt cggagacgag ttgtaaacaa tgcacaattt aaaacacaaa acacaaaaac
    11221 aagagcttag agatgtccct gatgctgaat aatttcaaaa aacaattatg taaaattgat
    11281 tttatatttt tgtgaaagga atacggagca tttgaaatca gaacaaatat gtatgaataa
    11341 tcaactgata attctcatag ttttatttgt gtaccttgta atatcggcat attccgtact
    11401 ctggatttca cggaacaagc tgttggaacg ggcgcagaaa atccggaaca tttgcattac
    11461 actgttgttg ccattctgga ttatcattgt gcggtcggca ttaaagccat tcaagtacaa
    11521 agaaaccaat gaccgcggct ggatcaggca ggaacacgaa ggcagtgtac gcggaacctg
    11581 gattggaggc atgtttaatc aatccgggaa atagcattaa aaatagatgt gaaaaaatcc
    11641 aaattcaggc agagaatatg aaatatgaat ttttaaattc tccgtataaa aatgtatata
    11701 tttgtttatt tttacgccaa gaaatgattt atcgttaacc aacacgggaa tatgagcagc
    11761 ataaaattat ttgatgaaat gcaaatcaga gttgtatgga gcaccactaa acaaaaatgg
    11821 tattttgcgc tttctgatgt ggttatagct ctgacaaata ctgttaatgt gaaacagtat
    11881 atcaaatata tgaaaaagcg cgataaggag cttgcgcaga actggtatca gtttgttaca
    11941 ttgatgcctg ttgaaacacc tgggggactg caaaacatga aatgtgccag caaagatcaa
    12001 ttattacgca tcattcaatt agtcaggtcg agtaaaaaac agaaatttaa aatttggctt
    12061 gcaaaggtta gttctcagaa aaagaacaaa tgatacccga tcgaactgaa acacagttta
    12121 taacagcgcc catgcggagt ttgaagcagc atgcggtgga gttgaacagc tgtgtgtttt
    12181 atggaaaatg gcattggttc tggggctact gggatgcttt cagttgggtg tatggacttt
    12241 cggaagagga aatccgggat ttcatgaaca gatatcagca tgccagagac aacatacaga
    12301 atattatttc tgttatggaa aacggcaggt taaaagataa gctgacaagt gaatatcgtt
    12361 ttttcccgga atcgaatgcc ggacaatatg tcaatagcca ttggtatctc tggctgtttt
    12421 tcattctaag tccaataaag aaaatcagaa aaacccatca gttcaaacgc tttcttgatg
    12481 atatagcgag tacatcggag cggatgatca attacactga aaacgctgcg tggctcgaaa
    12541 tggttccgga gaaagaggat catggattgt aatgagtaat gaatgcaggg tgattccaca
    12601 aaaaactctc agaattttat tgataagtgt tgataatata ccaataattg gtatatttgc
    12661 aaaaacaacc ggaaaatggg aaaaaataca tcgatattac tgggcgatta ttttgaaaag
    12721 tatgtgaatg agcagctgcg tacgggaaaa ttcgctacag taagtgaagt gatacgagcg
    12781 gctttgcggt tgatggaaag cgaagaaaat aaatcgaaaa cattggtgaa ggaattaaag
    12841 gccggcgaaa gaagcaggaa agtaaaaaac ttcgaccggg ccgaaaacct gaaaaaactt
    12901 catgctgaaa acactgtacg atgaagtacg aaatcagcga aaaagcgatt gaagacatca
    12961 accagatttg gttgtatact tttgaaaact ggccacagga gcagaccgac agatattata
    13021 atcagatttt tgacgaaatt gaatttattg ccgccaattt cgaagatggc cgatcaatgg
    13081 aacatatccg taaagggtat cgtgcaggta aagtgaaatc gcacctgata ttttaccgga
    13141 agtcgaagaa aaatgttgtc gaaattatca gggtgctgca tcagcacatg gatattgaga
    13201 atctgatgag tgaatgatct gttaaaagta atatttaaaa gctggctaaa aaataatgcc
    13261 gcaggaaaaa acacttaatc agaaattcat aaacatcagt cagggcaaac gcatctaaat
    13321 ttcaagaagg ctaaacaaat attcatttga ccatc
//
LOCUS       DAOPKE010000075        13216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4940_length_13216_cov_11.359927, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000075 DAOPKE010000000
VERSION     DAOPKE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_13216_cov_11.359927"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1603
                     /locus_tag="PKY63_09515"
     CDS             56..1603
                     /locus_tag="PKY63_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00891.1"
                     /translation="MKKSFLFITMLFPLILQAQILGPEAFNSDILVHGTSAANTVWFA
                     PEYNPPIAYISSGGCPGGRIGYSGSWNSYWGNFVRLPQINASGLDSIKLTFDVSHSYF
                     AAHTDDWCRFYVWADGAYRKIVSAVRINGSNVLYDSGANGKGFKFTETRSCAATEIVF
                     DLSTIIDKTSILIFLEPSCGYDNSNTFYVYFDNISIVNAGTTTFSVNCTPDTAFCIDH
                     PAWILNGCTPTGGMYSGNGVSGGVFSPAMAGAGTHSIKYKANDGMTTDSCYFNITVNP
                     VPVLNITVAPNDTLCYGIPLTLTASGAQTIQWSNGVQNGIAFIPDTQPYIVIGTDANG
                     CQSDSAIDIVVNALPSVTLNLPDDSVCGYSMPVFPTYPLSGGLPAGGIYSGNDVSGSN
                     LIGDVVMGNHYSVVNYTFTNNHGCSNSATDSIYLYNCDTGFPEMQDLSVCIYPNPAAD
                     AVYIIHNNSINHLEIVSVDGKMVWSTFVNPESNTTTADISELEKGSYFLRWRSKDAAG
                     AISFVKE"
     gene            1647..3899
                     /gene="hypF"
                     /locus_tag="PKY63_09520"
     CDS             1647..3899
                     /gene="hypF"
                     /locus_tag="PKY63_09520"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPF00892.1"
                     /translation="MLTKLNTYLIHIKGLVQGVGFRPFIFRVASDFNLNGEVENRNDG
                     VFIKINCDEKTLEAFVSAVRNTAPPASSIESIETIQTEFIDFSDFQIVKSNTVSNRVT
                     EVSPDIAVCDDCLRDMKTQPNRIDYPFINCTNCGPRFTIIKDLPYDREKTTMREFIMC
                     GDCRTEYTDVRDRRFHAQPVACSVCGPEYTLHYEGKTIKGISEILNQLGKLFHEKKII
                     AIKGIGGFFLACDALDEEAVQRLRNLKNREGKPFAVMFSSLQKLKEYAYVDAVEEKSL
                     TSLKRPIVLLNSKKELAPSVCVGFGTVGAMLPYMPLHYLLFEKTKLEVIVLTSGNISD
                     EPIITDNSDALNILTKVADAVVTYNRDIYNRADDSVVTVVNDKERISRRSRGYAPTPI
                     NLKLNVDGILATGAELVNCFAIGKHDQAILSQHIGDLKNMETYAFYSESLKRYKQIFR
                     FEPQVVVSDLHPDYLSTRYATESGLAHVKVQHHHAHIASCMAEHGLNETVIGISFDGT
                     GLGDDGHIWGGEFLICDLNKYKRFSHFGYIPMPGGDKVTKEPWRMAVSYLYHFFGNEF
                     HNFKLPFLRHIAPVTLEMLCQAIDKKINTPLTSSSGRLFDAVAALINLCPVSQFHAEA
                     PMRLESVIDKSVDDSYPFAINGTMCFEPTFRAILQDMKKQTGIPVIAAKFHNMIVAAT
                     IEMASAMKKECDSNKVVLSGGTFQNKYLLGKLEHKLAKQGFEVFTHQRIPTNDGGIAL
                     GQLLIAAARR"
     gene            4179..4424
                     /locus_tag="PKY63_09525"
     CDS             4179..4424
                     /locus_tag="PKY63_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPF00893.1"
                     /translation="MCLSIPSKVISINGDMAIVSVGGTEYEASLQLLTDVKVGDYILL
                     HTGFAIQKISEEEAIETLKVFEEFDDLNEQIKPELDI"
     gene            4550..4921
                     /locus_tag="PKY63_09530"
     CDS             4550..4921
                     /locus_tag="PKY63_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00894.1"
                     /translation="MVELFESTKQNISQHINNIFKEGELSENSTIKEYLTVQNEGLRK
                     INRTQIAELFIRDVKTIGKHINNALSEELCDFQVVAKFATTAFGSENTFMKGLNYVLL
                     RIRCLFGFNIMPQFNNTLVII"
     gene            4918..5790
                     /gene="rhuM"
                     /locus_tag="PKY63_09535"
     CDS             4918..5790
                     /gene="rhuM"
                     /locus_tag="PKY63_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HPF00895.1"
                     /translation="MNNEIILYQPDEKSSRLEVRIENETVWLTQAQMVELFESTKQNI
                     SLHTNNIFKDHEIEEDSVVKYSLTTAADGKRYKTKYYNLDVIISVGYRVKSQRGTQFR
                     IWANKVLKDYLLKGHAINQRFERIENDIHFLKNKTDEFDLQIRTSLPPNEGIFFEGKV
                     FDAYVFVAGLIKSAKKSIALIDNYVDESVLTMLSKRKSGVICDIFTSHTGNQLMLDVE
                     KHNAQYSEIALHQFSMSHDRFLILDNSIVYHIGASLKDLGKKWFAFSKIGIDANEMLQ
                     KLRKDSKKVSKNKK"
     gene            5787..6866
                     /gene="hypD"
                     /locus_tag="PKY63_09540"
     CDS             5787..6866
                     /gene="hypD"
                     /locus_tag="PKY63_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPF00896.1"
                     /translation="MKYIDEYRDKELVDKLVHQIRTISKKDIVLMEVCGGHTWAIQKF
                     GIPSLMPANVRLISGPGCPVCVTDRRFLDHAIALSRIPDVIITTYGDLIRVPGSTSSL
                     AGEKSKGADVRIVYSVLDALKIAKENPTKKVVFLGVGFETTTPPSAAGLINAKKENLK
                     NFFLLSSHKIMPPAMGALIDEGVAINGYIAPGHVSVITGTKIFEALPSKYHVGCVVCG
                     FEPVDILQTIYMLIKQHEENDYKVEIQYRRAVTPEGNVKAQEAMQEVFSLRDEWWRGL
                     GTLANSGLGVNEKYAEFDAERMISVEVEPTREDKGCICGEILKGLKKPNDCKLFGTAC
                     TTSNPVGACMVSHEGACNAYFRFNK"
     gene            6866..7879
                     /gene="hypE"
                     /locus_tag="PKY63_09545"
     CDS             6866..7879
                     /gene="hypE"
                     /locus_tag="PKY63_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPF00897.1"
                     /translation="MEKNILLGHGSGGRLSHELIEKIFYKHFSNPVLGQQSDSAVVPC
                     EGEMLSFTTDSYVVTPVFFNGGNIGKLAVCGTVNDLAVSGATPKYLSSAFIIEEGLSA
                     EELETIVATMAEEARKAGVIIATGDTKVVNKGQCDKVFINTAGIGFIKSEHKSIGTGE
                     LIRPGDKILINGSIADHGMTIMAARNFGNFNTDIKSDCAALNHMIRAILDAGCKVKFM
                     RDATRGGIATVLCEMAEHNNTGVHIHEEAVVINENVRGMCELLGFDPFYVANEGKVII
                     VVDASDADKALQIMKNNEFGKESTIIGEITEEHKGKAVLKTSIGGTRIIDMLAGEQLP
                     RIC"
     gene            8011..8775
                     /locus_tag="PKY63_09550"
     CDS             8011..8775
                     /locus_tag="PKY63_09550"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HPF00898.1"
                     /translation="MKNKYLNYVSDKHLLKCIEELHHAYLKAKNNISKKTFYLNKVDT
                     IKLTFDAKFNSITEENLIQSEILRQIDKSINNSIGTFHEKVLGGIKGYESGNLSGYDI
                     KANDDTLFADIKNKHNTMNSSAAEALFQKLARYADDYKKAKCYWVQILAKSSFCELWS
                     GDINGKEYSHSRVYKISGDQFYHLLSGTPDALYQLYKVMPIAINDYLKTVDNNLEVSE
                     NSALDEIKNGINKSNRSILDQITFENYSYYLGFDKL"
     gene            complement(8751..9989)
                     /gene="dcm"
                     /locus_tag="PKY63_09555"
     CDS             complement(8751..9989)
                     /gene="dcm"
                     /locus_tag="PKY63_09555"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPF00899.1"
                     /translation="MNEYLTLSEAAELIGKSKETLRRWDREGKLSAVREPISNYRVYK
                     REQVEMLFTDFVKSEAEDIIVNQVAAQNHYTVIELFAGAGGLAVGMEKAGLKCVALNE
                     IDKWACQTLRENRPKWNVLEGDIKNFSFGEYFNKVDVVTGGFPCQAFSYAGKKLGLND
                     ARGTLFYEFARVVKEVNPPICIGENVRGLLSHENGKTLQGMISILDEIGYNVLPIQVL
                     KAVNYRVPQKRERLILVGIRKDINAKFEYPKPYKKIYTLKDALKAGELFETDVPKSKG
                     ATYPQSKKVVLDLVPPKGYWRDLPLNIQKEFMGGSFHLGGGKTGMARRIGWDEPCLTL
                     TCSPAQKQTERCHPDETRPFTVREYARIQTFPDDWDFSGSLAQQYKQIGNAVPVNLAK
                     EVGYSIVNFLNQYYSLSKPR"
     gene            complement(10369..11436)
                     /gene="hydE"
                     /locus_tag="PKY63_09560"
     CDS             complement(10369..11436)
                     /gene="hydE"
                     /locus_tag="PKY63_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPF00900.1"
                     /translation="MIDGVFSGNLDFKKIKQLLLLEDEKQIQQLLEKARATRDNTIGN
                     KVYLRGLIEYSNRCAKNCFYCGIRAGNHHAVRYQVSDDEVVEAAQYALNHRYGSIVLQ
                     SGERCDKEFTSHVSSLIQKIHHATGNNLRITLSCGEQSEEVYREWFRLGAQRYLLRIE
                     ASNPELYAKLHPADHSFENRTKCLKTLVDIGYQAGTGVMIGLPFQTIDDLANDLLFIR
                     NSGVVMVGMGPYIEHAETPLYQYKDSLLPVEKRMELALKMIAILRLMRPTINIASTTA
                     LQTLDAHGRELGLRAGANVIMPNITPVSFKQEYNLYEGKPGLHDEPDEVENTIEKLII
                     SADCEVGWSEWGDSKFYSKKS"
     gene            complement(11447..12667)
                     /gene="hydF"
                     /locus_tag="PKY63_09565"
     CDS             complement(11447..12667)
                     /gene="hydF"
                     /locus_tag="PKY63_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPF00901.1"
                     /translation="MSKGKDLKPHIGIFGRRNNGKSSFINVMTGQEVAIVSEHAGTTT
                     DPVKKSVEIFGIGPVIMVDTAGIDDTGDLGEKRIRKSLQSIKTVDCAVLLIAGNRFGS
                     HEIALINEFKNNDTPFIIVHNKSDVEALSKETIDEIARQTDAVVLDFSARTKSGFDEV
                     VEALRQSVPDKAYMYKSLIGDLIREGDYVLLVTPIDSEAPEGRMILPQQMAIRDVLDN
                     NAICIVLKETELENFFKTSCIRPALVVTDSQAFGYVKSVVPEDILLTSFSIVFSRIKG
                     DFENYIKGTRTIDELQDGDKILILESCTHHISCDDIGRVKIPMWLSKFTGKKLYFTTI
                     SGLGDLPDNCKDYKLVIQCGGCMVTRKQVLNRLKPFTDKEVSITNYGMAIAYLNGIFE
                     RALQPFCSNDCEKQ"
     gene            complement(12686..>13216)
                     /locus_tag="PKY63_09570"
     CDS             complement(12686..>13216)
                     /locus_tag="PKY63_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPF00902.1"
                     /translation="DEDFARLVAILRLAVPYTGMILTAREPVEVRNELLQFGVSQIDG
                     GTKLELGSYSDHDEHEQNLNKGQFRINDNRTLAEVISELLDQGMIPSFCTACYRLGRT
                     GEHFMEFSVPGFIKRFCTPNAILTLSEYLVDYGNEENKKKGWALIEKELAELPNADEV
                     RKRIEEIKNGKRDLYF"
BASE COUNT         3918 a         2922 c         2704 g         3672 t
ORIGIN      
        1 actgatttta aattgcacat aaacatctaa tttctatttt tacataaaaa agtatatgaa
       61 aaagagtttt cttttcataa caatgctgtt tccattaatt cttcaggcgc aaattttagg
      121 cccggaggca ttcaacagcg acatactcgt tcatgggaca tcagcggcaa acactgtatg
      181 gtttgctcct gaatacaatc caccgatagc ttacatttca agcggcggat gtccgggtgg
      241 tcgaatcggc tattccggat catggaatag ttattggggc aattttgtac gacttccaca
      301 aatcaatgca tccgggctcg atagcatcaa actgacattc gacgtttctc actcctactt
      361 tgcagctcac accgatgatt ggtgccgctt ttatgtctgg gccgacggtg catatagaaa
      421 aattgtttca gcagtcagaa ttaatggcag caatgtatta tatgattcag gtgcaaacgg
      481 caaaggtttt aaattcactg aaacacgctc ctgcgctgcc accgaaatag tcttcgacct
      541 gagcaccatc atcgataaaa cgtccattct gatttttctc gagcccagct gcggatacga
      601 caacagcaac acattttatg tttatttcga caacatttca atcgtaaatg caggtacaac
      661 gacattttcg gttaactgca ctccggatac cgcattttgc attgaccatc cggcatggat
      721 attaaacgga tgcactccta caggaggcat gtacagcgga aatggagttt caggtggtgt
      781 attcagtccg gctatggctg gtgcagggac acacagcatt aaatacaaag ccaacgacgg
      841 aatgaccacc gacagctgct atttcaatat caccgtaaat ccggttccag tgctgaatat
      901 cacagttgca cccaatgata cattgtgtta tgggatcccg ctgacgctga ctgcaagcgg
      961 agctcagact attcaatgga gcaacggcgt tcagaacggc attgcattta ttcctgatac
     1021 gcagccatac attgtgatcg gcaccgacgc aaacggctgc cagagcgatt ctgcaataga
     1081 tattgttgta aatgccttac cgtctgtaac tttaaatctg cccgatgact cagtctgcgg
     1141 ctattctatg ccggtttttc caacctatcc cttgtcaggc ggactgcctg caggcggcat
     1201 ttacagcggc aacgatgttt caggttcaaa cctgatcgga gatgttgtaa tgggtaatca
     1261 ctacagtgtg gttaactaca catttactaa taatcatggt tgttcaaact cagcaaccga
     1321 ttcaatttac ctgtacaatt gcgatactgg cttcccggaa atgcaggatt tatccgtttg
     1381 tatttatccg aatccggctg cagatgctgt ttacatcatt cataacaaca gtatcaacca
     1441 tctcgaaatt gtcagcgttg atggaaaaat ggtctggtca acttttgtga atcctgaaag
     1501 caatacaact acagctgata tttcagaact ggagaaagga agctattttt tgcgctggag
     1561 aagcaaggat gcagcgggcg ctatttcttt tgtgaaagaa taggaaactg tttcttctct
     1621 aattttaata actttgcaac ttgattatgt tgacaaagtt gaatacctat ctcatccata
     1681 tcaaaggcct tgtgcaaggc gtcggtttcc ggcccttcat atttcgtgtt gcatcagatt
     1741 ttaatttgaa cggcgaagtg gaaaaccgca acgacggcgt ttttattaaa attaattgtg
     1801 atgagaaaac gctggaagct tttgtgagtg cagttcgcaa taccgctccc ccggcctctt
     1861 ccatcgaatc gatcgaaaca atacaaaccg aatttattga tttttctgat tttcagattg
     1921 taaaaagcaa tacggtttcc aatcgtgtaa ctgaggtcag tcctgatatt gcggtgtgtg
     1981 atgattgtct gcgcgacatg aaaacacaac caaaccgcat cgactatcct ttcattaact
     2041 gcaccaactg cgggccgcgc ttcactatca taaaagatct gccctacgat cgggagaaga
     2101 ctacgatgcg tgaattcatc atgtgcggcg actgccgtac agaatacacc gatgtgcgcg
     2161 accgccgctt tcatgctcag cccgtggctt gctcggtgtg cggacctgaa tacaccttgc
     2221 attacgaagg caaaacaata aaaggaattt cagaaatcct gaatcagttg ggaaaacttt
     2281 ttcacgaaaa aaaaataata gctatcaaag ggatcggcgg cttctttctg gcttgtgatg
     2341 cgctcgacga agaagctgta cagcgactgc gaaatctgaa aaacagagaa gggaaacctt
     2401 ttgctgttat gttttcgtcg ctgcaaaaat taaaggaata tgcatacgtc gacgcagtcg
     2461 aagaaaaatc actgacatcg ctcaaacgcc cgattgtact gctgaacagc aaaaaggaac
     2521 tggctccttc ggtctgtgtc ggattcggaa cagtaggcgc gatgctgcct tacatgccac
     2581 tgcattattt gctgtttgaa aaaacaaagc ttgaagtgat tgttctcacc agcggcaata
     2641 tctccgatga accgatcatt actgataact ccgacgcgct gaatattctg accaaagtgg
     2701 ccgatgctgt ggtgacctac aaccgcgata tttacaaccg cgccgatgat tcagtggtga
     2761 ccgttgtcaa tgacaaagag cgtataagtc gccgctcgcg cggttatgcg ccgaccccca
     2821 tcaacctgaa actcaatgtc gatggcattc tggccaccgg agccgaattg gtaaactgct
     2881 ttgccatcgg caaacacgat caggccatac tgagccagca tatcggtgat ctgaaaaata
     2941 tggagaccta cgcattttac agcgaaagtc ttaaacgcta caaacagatt ttccgcttcg
     3001 agcctcaggt tgtggtgagc gatctgcatc ccgactacct gtcgacccgc tatgcaacag
     3061 aaagcggact cgcgcatgta aaagtgcagc atcatcacgc gcacattgca tcatgcatgg
     3121 ccgaacacgg actgaatgaa acggtaatcg gcatcagctt cgacggtact ggtttgggcg
     3181 acgacggcca tatctggggc ggcgagtttt tgatttgtga tttaaataaa tataaaagat
     3241 tcagccattt cggatatatt ccaatgccag gcggagacaa ggttacaaaa gaaccgtggc
     3301 gcatggcggt ctcctatctg tatcactttt ttggaaacga atttcataat ttcaaactgc
     3361 cttttctacg ccatattgca cctgtaacat tggaaatgct gtgtcaggcc atcgataaaa
     3421 aaatcaacac accccttact tcaagctccg gccgtttgtt tgatgcagtt gccgcactga
     3481 tcaatctttg tccggtgagt caattccatg ccgaagcacc catgcggctt gagtcagtca
     3541 ttgataaaag cgttgacgat tcatatccat ttgccattaa cggaacaatg tgctttgaac
     3601 ctacattcag agccattctt caggacatga aaaagcaaac aggtattccg gtcattgctg
     3661 caaaatttca caatatgatt gtagcagcca caatcgaaat ggcctcagca atgaaaaaag
     3721 aatgcgattc caataaagtt gttttgtccg gcgggacatt tcagaataaa tatctgcttg
     3781 gaaaacttga gcacaaactg gctaaacaag gatttgaagt ctttacacat cagcgcatcc
     3841 cgaccaacga cggtggcatt gcgctggggc agctgctgat tgcagcagcg aggcgctagg
     3901 taataacccg aggatgtctc aaattttcat tggcggagca aaaacaacat tccattcccg
     3961 ctgatggcgg aataaactcc cgaaagcgac gaaggagatg aaggtcatgg aaccgttgtt
     4021 tttgttattg ttacgacggt tccatgtccg ccagccatgt gctgagcctg acgaagtatg
     4081 gcggacggtg gaatgtcgta acaattagcc gttttgagac atcctcgtgg aaaacaattt
     4141 cttactacct ttgtaccttg aattaaaatg agattgatat gtgtctgagc attccctcca
     4201 aagtgatttc aatcaacggt gatatggcca ttgtttcggt cggcggcacc gaatatgaag
     4261 cttcattgca gctgttgacc gatgtgaaag tcggcgatta cattttactg cacaccggct
     4321 ttgccattca gaaaatcagc gaagaagaag caattgaaac cctcaaggtt tttgaggaat
     4381 tcgatgatct caacgaacag atcaaacccg aactggatat ctgatttcac tgtcttttct
     4441 gactctgcct gttgtgaaca atgagattgt catatttcaa cctgatgaaa agtcttcccg
     4501 gcttgaggtg cgacttgaga atgagactgt gtggcataca cagactcaga tggttgagct
     4561 gtttgaatca acaaagcaga atataagtca gcatataaac aatattttta aagaaggtga
     4621 actttctgaa aattcaacta tcaaggaata cttgacagtt caaaatgagg gattgaggaa
     4681 aataaacaga actcagattg ctgaactgtt catcagagat gtaaaaacca tcgggaaaca
     4741 tattaacaat gctctaagtg aagagttatg cgattttcaa gttgtcgcaa aatttgcgac
     4801 aactgcattt ggtagcgaaa ataccttcat gaaaggttta aattatgtcc tattaagaat
     4861 tagatgcctt tttggattta acattatgcc gcaatttaat aatacgttag ttattatatg
     4921 aacaatgaaa taattctata tcagcctgat gaaaagtcat cccgcctcga agtaaggatt
     4981 gagaatgaga ctgtatggct tactcaggca cagatggttg agctgtttga atcaacaaag
     5041 cagaatataa gtcttcacac aaacaacatc tttaaagatc atgaaataga ggaagattca
     5101 gttgtcaagt attccttgac aactgctgct gatggaaaac gttataaaac aaagtattac
     5161 aatttagatg ttattatttc agttggttat cgtgtcaaat cacagcgcgg aacccaattc
     5221 cgcatctggg caaacaaagt gctgaaggac tatctgctga aaggtcatgc catcaatcag
     5281 cgatttgaaa gaattgaaaa cgatatccat tttctgaaaa acaaaaccga cgaattcgac
     5341 ctgcagatca gaaccagcct gccccccaat gaagggattt tctttgaagg aaaagtcttt
     5401 gatgcttatg tttttgtagc aggactcatt aagtcggcaa aaaaatccat tgctctaatc
     5461 gacaactacg tggatgaaag cgtactcaca atgctctcaa aacgcaagtc cggtgtaata
     5521 tgcgacattt tcacatcaca cacaggcaat caacttatgc ttgatgttga aaagcacaat
     5581 gctcagtatt ctgaaatcgc gctgcatcag ttttccatgt cgcacgatcg ttttctgatt
     5641 ctcgacaaca gcattgtgta ccatatcggt gcatccttaa aggatcttgg aaagaaatgg
     5701 tttgcgtttt caaaaatcgg aatcgatgca aacgaaatgc tgcaaaagct ccgaaaagac
     5761 tcaaaaaaag taagtaaaaa caaaaaatga aatacatcga cgaataccgc gacaaggaac
     5821 tggtggacaa actggtgcat cagatcagga ccatcagtaa aaaagacatt gtgctgatgg
     5881 aggtctgcgg cggccacaca tgggccattc agaaattcgg catcccttcg ctgatgcccg
     5941 caaatgtacg tctgatctca ggtcccggct gtccggtctg tgttaccgac cgtcgctttc
     6001 ttgatcatgc catcgcgctg agccgcattc cggatgtcat tatcaccacc tacggtgatc
     6061 tgatccgagt tcccggctcc acatcatctc ttgcaggtga aaaaagcaaa ggcgctgatg
     6121 tccgcatcgt ttattctgtt ttggatgcac tgaaaattgc caaagaaaat cctacaaaaa
     6181 aagtggtctt cctgggtgtc ggtttcgaaa caacaacgcc tccaagcgcg gccggactca
     6241 tcaatgcaaa aaaagaaaac ctgaaaaatt tctttttgct gagttcgcat aaaatcatgc
     6301 cgcccgctat gggagcgctg attgacgaag gtgttgccat caatggttac atcgctcccg
     6361 gccatgtaag cgtaatcacc ggaacaaaaa tattcgaagc gcttccatcc aaataccacg
     6421 tgggctgcgt cgtttgcggc ttcgagccgg tcgatattct gcagaccatt tacatgctga
     6481 tcaaacagca cgaggagaat gattacaaag tcgaaattca gtaccgccgc gccgttacac
     6541 ccgaaggcaa tgtgaaagca caggaagcca tgcaggaagt tttttcgctg cgtgatgaat
     6601 ggtggcgcgg cctcggaacc cttgccaaca gtggcctcgg cgtgaatgaa aaatatgcgg
     6661 aattcgatgc agagcgcatg atttcagtag aagttgagcc aacgcgcgaa gacaaaggct
     6721 gcatctgcgg cgaaattctc aaaggcctca aaaaaccgaa cgactgcaaa ctgttcggaa
     6781 cggcctgtac aacatcaaac ccggtaggtg cctgcatggt ttcgcacgaa ggcgcctgca
     6841 atgcttactt cagattcaac aaataatgga aaaaaatatt ttactcggac acggaagtgg
     6901 cggacgtttg tcgcacgaac taatcgaaaa gattttttac aaacatttca gcaaccctgt
     6961 actcggccaa cagagcgatt ctgcagttgt tccttgcgaa ggggaaatgc tgtcgtttac
     7021 tacggattcg tatgtggtta ctccggtatt tttcaatgga ggcaacatcg gaaaacttgc
     7081 tgtttgcggc actgtgaacg atctcgcagt atccggtgca acaccgaaat atctcagctc
     7141 agcatttatt atcgaagaag gtctgtctgc tgaagaactg gaaaccattg ttgccaccat
     7201 ggctgaagaa gcccggaagg caggtgtgat catcgctaca ggcgatacca aagtggtgaa
     7261 taaaggtcaa tgcgacaaag tttttatcaa tacagccggc attggtttca tcaaatccga
     7321 acacaagtca atcggaacag gagaactgat ccgcccgggc gataaaattc tgatcaacgg
     7381 aagcattgct gatcacggta tgaccattat ggctgcacgc aatttcggca atttcaatac
     7441 cgacatcaaa tccgactgtg ccgcgttaaa tcatatgatc cgcgcgattc tcgatgccgg
     7501 ctgcaaagtg aaattcatgc gcgatgccac gcggggtggt attgcgactg ttctttgcga
     7561 aatggctgaa cacaataaca ccggagtaca cattcacgaa gaagccgttg tcattaacga
     7621 aaacgtgcgt ggtatgtgcg aactgctggg cttcgatcca ttctatgtcg ccaacgaagg
     7681 aaaagtcatc attgttgtgg atgcttcaga tgcagacaaa gctttgcaaa taatgaaaaa
     7741 caatgagttt ggaaaagaat caaccatcat tggcgaaatc accgaagaac acaaaggaaa
     7801 agctgtgttg aaaaccagca tcggcggtac ccgcatcatt gatatgcttg ccggcgaaca
     7861 gctgccgagg atctgttaaa aaggatgcct gattttttaa ttagattatt aaaatatagt
     7921 tcagaaacag gctaataaag tgcttgctac tgaaatattt ttttaaatta tttttatttt
     7981 tacatttgta ttagaaaaca tataagttgc atgaaaaaca aatatctcaa ctacgtatct
     8041 gacaaacatt tgctcaaatg tattgaagaa ttacaccatg cctatttgaa agcaaaaaac
     8101 aacatttcaa aaaagacttt ctatttgaat aaggttgata ctattaaact cacttttgat
     8161 gctaaattca acagtattac agaagaaaat cttatccagt ccgagatatt aagacaaata
     8221 gataaatcca ttaataattc aattggaact tttcatgaga aagtattagg aggcatcaaa
     8281 ggttacgaat caggaaatct aagcggttat gatattaaag caaatgacga tactcttttt
     8341 gctgatatta aaaacaaaca caatacaatg aatagtagtg ctgcggaagc actgtttcag
     8401 aagttagccc ggtacgctga tgattataaa aaagctaaat gttattgggt tcaaatattg
     8461 gcaaagagta gtttctgtga attatggagt ggtgatatta acggaaaaga atatagccat
     8521 agcagggtat ataagatttc cggagatcaa ttttatcatt tattatcagg aactcctgat
     8581 gcgctttacc aactatacaa ggtaatgcca attgcaataa atgattattt aaaaacagtt
     8641 gacaacaatt tggaagtatc tgaaaattca gcgttagatg aaataaaaaa tggcattaat
     8701 aaaagcaata gaagtatctt agaccaaatt acttttgaaa actatagtta ttaccttggc
     8761 tttgataaac tatagtattg gttcaaaaag ttgacaattg aatacccgac ttcttttgct
     8821 agatttacag gcacagcatt gccaatctgt ttatactgtt gagccaaaga acctgaaaaa
     8881 tcccaatcat caggaaaagt ttgtattctt gcatattctc taacggtgaa aggcctggtt
     8941 tcatcaggat ggcacctttc tgtttgtttt tgtgctgggc tacatgttag agtcaggcaa
     9001 ggctcgtccc agcctattct ccttgccatt ccagtttttc ctcctccaag atgaaagcta
     9061 cctcccataa actccttttg aatatttagt ggtaaatctc tccaataacc ttttggagga
     9121 actaaatcta atacgacttt tttactttgt ggatacgtgg ctccttttga ttttggtacg
     9181 tccgtttcaa atagttcacc tgcttttaga gcatctttga gagtgtaaat ctttttatat
     9241 ggtttagggt attcaaattt agcattaata tcttttctga ttccaactaa tattaaccgt
     9301 tctcttttct gaggtacacg ataatttaca gcctttaaca cctgaatagg caaaacatta
     9361 tacccaattt catccaatat agagatcatc ccttgaagtg tttttccatt ttcgtggctt
     9421 aataggccac gcacattttc accaatacaa attggaggat tcacctcttt aactactctg
     9481 gcaaattcat aaaacagagt tcctcttgca tcattaagcc caagtttttt cccggcataa
     9541 ctaaatgctt gacaaggaaa tccaccagtc acaacatcga ccttgttgaa atattcgcca
     9601 aaagagaaat tttttatatc cccttctaac acattccatt tcggtctatt ttctcttaaa
     9661 gtttgacaag cccatttatc aatctcattc aaagcgacac attttagacc agccttttcc
     9721 attccaacgg ctaacccgcc agcaccagca aacaattcta tgactgtata gtgattttgt
     9781 gcagcaactt ggttcacaat aatatcttcc gcttcactct ttacaaaatc agtgaacaac
     9841 atttcaactt gttcacgctt ataaactctg tagttactta ttggctcgcg tactgcggac
     9901 aatttccctt ctctatccca tcgacgaaga gtttctttgc ttttgccaat gagttcagca
     9961 gcttctgaaa gtgttaaata ttcattcata accaaattgt ttaaccttac acaatggtta
    10021 caaatttaca acaaaaaaaa atacaattcg gaaaaattaa aaattttcag gaaaactcta
    10081 aaaactatta cgcaaatgat tatgaattaa tttaacaact gtgttgatca gtaactttat
    10141 aaaaaacact tgaattcttc acaattagcc ggcgaacagc tgccgagaat ctgttaatac
    10201 aaaagataaa aggataaaga caaaaggaaa tacaatattc cacttactgc aaaactttta
    10261 tctttttact tttgtctttt atcttgcgca tttgtcttgc gcattagtct tgcgccactt
    10321 cggcaagctc agtgcggcgc ttttgtcttt aatcttgaaa tgtgtctttt agcttttctt
    10381 cgaataaaat ttcgaatcac cccactcgct ccagccgact tcgcaatcgg cgctgataat
    10441 gagcttttcg atggtgtttt ccacctcatc gggctcgtcg tgaaggcccg gttttccctc
    10501 gtacagattg tattcctgct tgaaagaaac cggagtaata tttggcatga ttacattggc
    10561 gcccgctctg agacccagtt cacggccatg tgcatcaaga gtctgcagcg ccgtggttga
    10621 tgcaatgttt attgtaggcc gcatcaggcg cagaattgca atcattttca gcgcaagctc
    10681 catgcgtttt tcaactggga gtaatgaatc tttgtattga tacaggggtg tttcggcatg
    10741 ctcaatgtat ggacccattc caaccatgac aactcccgaa ttccggatga acaataaatc
    10801 attggccagg tcgtcgatgg tttgaaaagg caatccgatc ataacccctg tccccgcctg
    10861 atagccaata tcgacaagcg ttttcagaca ttttgtccta ttttcaaaac tatgatcagc
    10921 aggatgaagt ttggcataca actccgggtt tgaagcttcg atgcgcaaca gataacgctg
    10981 agcacccaaa cgaaaccatt cgcgatagac ctcttcgctc tgttcgccgc aagaaagtgt
    11041 aatccgaaga ttatttccgg tggcgtgatg aattttctga atcaaagaag aaacatgcga
    11101 tgtaaactct ttatcgcaac gctcgccaga ttgcagcaca atggagccat atctgtgatt
    11161 aagcgcatac tgagcggctt ctaccacttc atcatcgctg acctgataac ggacagcatg
    11221 gtgattacca gcacgaatac cacaatagaa gcagtttttc gcacaacgat ttgagtattc
    11281 aattaaccca cgcaaataca ctttatttcc gatggtatta tcgcgtgttg cacgggcttt
    11341 ttccagcaat tgctgaatct gcttctcatc ctcgagcaga agaagttgtt tgattttttt
    11401 aaaatcaagg ttgccactaa aaacaccgtc aatcatgttt gcaaatttac tgtttttcgc
    11461 agtcattact gcagaagggc tgcagtgccc gctcgaagat accgttcaga tatgctatcg
    11521 ccatgccgta attagttatg gaaacctctt tatccgtaaa tggctttagt ctgttcagca
    11581 cctgcttgcg ggtcaccatg cagcctccgc attgtatcac cagcttgtag tccttacaat
    11641 tgtccggcaa atcaccaagg ccggatatcg tggtaaaata cagttttttt cctgtgaatt
    11701 tcgaaagcca catcggaatt ttcacacgtc cgatgtcatc gcagctgatg tgatgagtgc
    11761 aggattcaag aatcagaatc ttatcgccat cctgcaattc atcaatggtt cgggtgcctt
    11821 tgatgtagtt ttcaaaatct ccttttatcc tcgaaaacac aatactgaaa cttgtcagca
    11881 gaatgtcttc gggtacaaca ctttttacat aaccaaacgc ctgactgtct gtaacgacca
    11941 atgcaggccg gatgcaactg gttttgaaaa agttctcaag ctccgtttct ttcaaaacaa
    12001 tgcaaatggc gttgttatcc agcacatcgc ggatggccat ctgctgaggc agaatcatac
    12061 gaccttccgg agcttcgcta tcgatggggg ttaccagtaa aacataatcg ccttctctga
    12121 taagatcgcc aatgagcgat ttatacatat atgccttatc gggcacactt tgcctcaatg
    12181 cttcaacaac ttcgtcgaaa cccgattttg ttcttgcact gaaatcaagt acaacagcgt
    12241 cggtttgtct ggctatctca tcaattgttt ccttgcttaa agcttcaaca tccgatttgt
    12301 tatgtacaat aatgaaaggc gtatcgttat ttttgaattc gttgataagc gctatttcgt
    12361 gcgatccaaa tctatttcca gcaatcagca aaactgcaca atcgacagtt ttaatagact
    12421 ggagtgattt tcggatgcgc ttttcgccca gatctccggt atcgtcaata ccggcagtat
    12481 caaccataat gaccggaccg ataccaaata tttcaacgga ctttttcacc ggatcagtag
    12541 tagtaccggc atgttcagaa acaatggcaa cctcctgccc tgtcatcaca ttgatgaaag
    12601 agcttttacc gttattacgc cggccaaaaa tgccgatgtg cggcttgagg tctttacctt
    12661 tactcattgt ttcgtgtttt aacgattaga aatacaaatc gcgtttccca ttcttgattt
    12721 cttcgatgcg cttacgcact tcatcagcat tgggcaactc agccagttcc ttctcaatca
    12781 aagcccaacc cttcttttta ttctcttcat ttccataatc aaccaggtat tccgataaag
    12841 tgagtatagc atttggtgtg caaaatctct tgatgaaacc cggtaccgaa aactccataa
    12901 aatgctctcc tgttctgccc aaacgatagc acgcagtaca gaaggacggt atcattccct
    12961 gatccaataa ttcggaaata acttcggcca gtgttcggtt gtcattgatt cgaaactgtc
    13021 ccttattcaa attctgctca tgttcatcat gatccgaata actgccaagt tcaagcttgg
    13081 taccaccatc aatctgcgaa acaccaaact gcaacagctc gttacgcacc tcgaccggtt
    13141 cacgggcagt aaggatcata cctgtataag gcacagccag acgcaaaatg gcaacaagac
    13201 gggcaaaatc ctcgtc
//
LOCUS       DAOPKE010000076        13161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4966_length_13161_cov_10.638639, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000076 DAOPKE010000000
VERSION     DAOPKE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_13161_cov_10.638639"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..724)
                     /locus_tag="PKY63_09575"
     CDS             complement(2..724)
                     /locus_tag="PKY63_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF00903.1"
                     /translation="MDEIAGMPETPQKPVREVKNLVSVENAVVRHGDNLILSNVSFSI
                     GSGEFVYFLGRTGSGKSSVMKMLYADLPFEGTIGTVLGYDLNKIRTREIPLLRRKMGM
                     VFQDFQLLTDRNVRENLLFVLQATGWRDREAMDSRIDSVLAGVGLENKKDKMPHQLSG
                     GEQQKVVIARALLNDPELLLVDEPTGNLDPQSSEEVLNTLVELKKTGKAILMATHDML
                     VMEKYPSRILWFADGKVTDNKE"
     gene            complement(1329..1913)
                     /locus_tag="PKY63_09580"
     CDS             complement(1329..1913)
                     /locus_tag="PKY63_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00904.1"
                     /translation="MNRTIVCIILCAVMQLCSNPISTMKSTNSKSNDFKKCYSKNKKL
                     LLESKQTFITVMNKYEIQQVILKFNEGITVDCRWESNDGYISFFLDYNDLELLRNSTC
                     DSCSGEQTENAFQLINDTSFIETINRLKEINPVSIRIDTSGVFYGIGQAVSSNYIRNG
                     GVGGILIVNKKDYSNTNSLEKIDESSFIYEDIID"
     gene            complement(1916..3442)
                     /locus_tag="PKY63_09585"
     CDS             complement(1916..3442)
                     /locus_tag="PKY63_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPF00905.1"
                     /translation="MYGKINYIDFTTASLKNDLEFRYDPMGNRTMKIEYASTGAKYTF
                     YSRDAQGNIMATYTMTDDANDYVYLDEFDIYGSSRLGVNNIGKKMTELTWSGSSFSFT
                     TGNKQYELTNHLGNVLATISDHKLPFSSNVNDGTVDYFLADITSSQDYYPFGMIMPER
                     NWNSSSYRFGFNGKENDNEVKGTGNFISFEFRDYDSRLGRFVTIDPLSAKYPWQSPYA
                     FAANNPILYVDIMGLGPGDPPTSTKSDANSIIENASKDLTQSDFYKNVSPEKFLSDLR
                     SRVQNPTAFNQGHGTNFCWAAACLSYVWEKDPAGMSKAMINLYKTGEFVYFNGSYTTR
                     STPDDTYEAIGSSTFSNNADFNNVIDQMSTMTAASGLNCGIIHFDNDYDPGDEEDPMW
                     SGGDLWKAKMLWNKMGYDVSIHGIDAGTWFNLDKGGLVTKLLNSSDIVLYVNSDAFKN
                     GQRIGNFFGTHFIRVDLISTLSDSYDIHYWDYGQWKYRWGISYGLFNASTYGIIQIPR
                     "
     gene            complement(3675..4703)
                     /locus_tag="PKY63_09590"
     CDS             complement(3675..4703)
                     /locus_tag="PKY63_09590"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF00906.1"
                     /translation="MNPWIAVPDETKRRAYAETAEKTGLSAFAAEKDWWVVQTLSVVF
                     GMDVSAHLVFKGGTSLSKGWKIIDRFSEDIDLVLDRSFLGFDGELSKNERGKLRKETG
                     LYVDNVFYPELIGEFASKGYDNVRLELVKEKQSDLDRKISIYYPNVIEAPGYLQPAVQ
                     IEISCRSLREPFTMRSIIPHLDEVFPDAGFIQSPAIIPTVNPERTFLEKIFLLHEEFH
                     RPAEKRRVNRLSRHLYDVVKLSKTSFAEKALADRALYETIVNHRYHFTRVGEVNYNLH
                     QPQTINPIPPAELMEAWKADYNVMIEQMIYEENPPAFDEIISDLKNLTKKINSLEWKF
                     SLEFPQPK"
     gene            complement(4700..5290)
                     /locus_tag="PKY63_09595"
     CDS             complement(4700..5290)
                     /locus_tag="PKY63_09595"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HPF00907.1"
                     /translation="MILAKIKKAKRGTLFFTEDFLSFGSAKAVAKALERLVGQGSISR
                     VARGIYARLESDRILGTITPGTEVIAEAISRRDKSRIIITGAAALNALGLSTQVPINV
                     VYLTDGAARRISIGKRKIIFKKTTPRNLAAIGKISSVVIQALKDIGKDNVTEAEIKVV
                     LKHLKKEDPYRLEHDIRLAPEWIRIIMRKAIKKSKS"
     gene            complement(5983..6741)
                     /locus_tag="PKY63_09600"
     CDS             complement(5983..6741)
                     /locus_tag="PKY63_09600"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPF00908.1"
                     /translation="MKTIFHRNEVVESTNQWAMSQVNEVLNNELHIFSAAFQSAGRGQ
                     GDHTWESETGENILMSILLKNPAVKVADQFVLSQIIAVAAHNYVSAELPGAEVSIKWP
                     NDIMVDSRKIAGILIENSLKGNDILATVIGIGVNVNQIHFAGDLQAAVSLEMMDGKKR
                     DIETEINKLTGAFLTSYENFLALGAEKTFWSYDGRLFGVGTDQNFKLGDDILKCRILG
                     SEPNGQLRLKMPDGKERTFFHHEIAMCYRDDLLI"
     gene            6826..7179
                     /gene="rsfS"
                     /locus_tag="PKY63_09605"
     CDS             6826..7179
                     /gene="rsfS"
                     /locus_tag="PKY63_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPF00909.1"
                     /translation="MVKTVKDPLVSKLTKALKSKKAQNIVVLDMRETGQNVCDFFVIC
                     HGVSGVQTQGIAENAIRELRKEEGFKPIQVEGLSNAEWILVDYGNVVAHIFREDMREF
                     FRLEDLWADAKITTI"
     gene            7204..9267
                     /gene="ftsH"
                     /locus_tag="PKY63_09610"
     CDS             7204..9267
                     /gene="ftsH"
                     /locus_tag="PKY63_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPF00910.1"
                     /translation="MKDKPKDNPNQGPKKKFNFYWIYAIMAIAFFAMYFYGSEPQEPK
                     EIMWQRFENEMLAKHHIEKIVVVNKEFAEIHIKKDIVTSDSSYKDAVKGKENIGPHYT
                     MKFLSPESFQTQLAAAEDRIFAKDTVGKTPQQVLELEKTRHVNVVPEERTNWGSTILV
                     NWILPIAIVLGIWFLFMRMMNRGSGGGQIFNVGKSKAQLFDRDTQVSITFKDVAGLEE
                     AKVEVVEVVDFLKNPKKYTSLGGKIPKGVLLVGPPGTGKTLLAKAVAGEAKVPFFSIS
                     GSDFVEMFVGVGASRVRDLFRQAKEKAPCIVFIDEIDAIGRARGRANITGANDERENT
                     LNQLLTEMDGFSTNAGVIILAATNRADILDKALMRAGRFDRLIYVELPDLHEREAIFN
                     VHIKPLKLSEDVKSDFLAKQTPGFSGADIANVCNEAALIAARNGKKAIEKQDFLDAID
                     RIIAGLEKRNKIISVDEKKTIAYHEAGHASVSWLLEHAHPLVKVTIVPRGKALGAAWY
                     LPEERSITTWNQIYDEMTSALGGRASEEVNFNEVSTGALNDLEKVTKQAYASVAYFGL
                     SKKLGNISFFDSSGSQEFYFTKPYSEKTAEVIDSEVHNIVESAYQRAKDLLIANREKV
                     SQLAKLLLEKEVIFREDVEHIFGPRPWADKETTKHVAPKVIETSSEETPEEKPEDNTK
                     ADTKE"
     gene            9280..9921
                     /locus_tag="PKY63_09615"
     CDS             9280..9921
                     /locus_tag="PKY63_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPF00911.1"
                     /translation="MKQFEHIELREKVLKRIRNALINKTELNAEQLNVEIAAFKPFQQ
                     AIPFEFAKNFTDAGGFFYYSENRQELKIAINTLLNEKGYMSVYCSNDDLSSLVNTGVT
                     AVLTELGDLPATPVVITECEFLCARTGSILLSSFLSCGRRGIATNDALIVVAKIDQIL
                     PDIQDAIQAMTEKYSEEFPSQLTFVTGPSCTNAIENNFIRGAHGSKEVYVFLH"
     gene            9940..10794
                     /locus_tag="PKY63_09620"
     CDS             9940..10794
                     /locus_tag="PKY63_09620"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPF00912.1"
                     /translation="MKNFLVRSASAVVYAGLLIAGSLWWSPLLYLTLTAFLIIGLLEL
                     KKLLQTDIHRILLVIWSIIGVLFMLITVALFDRTLIDNDFDSEALTPLLFFSPFLLAF
                     IILIISTISRKPAQGLLSFFSISIVYLLIPFMFMIGIQNAVFADSQPWLLVLLAAIWI
                     NDSMAYITGSLIGKHKLIERISPGKTWEGFLGGLIFTIATLLIINQIFFDFDAISLAI
                     IALVIVMSGTIGDLLESKLKREAGVKDSGNIIPGHGGILDRIDSLLVAAPFTGIALLI
                     NEIIIHWH"
     gene            10794..11459
                     /locus_tag="PKY63_09625"
     CDS             10794..11459
                     /locus_tag="PKY63_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPF00913.1"
                     /translation="MRTHPAGKDVINWVVTILGTIALMVIMFVPFKLFYVICGVLFIL
                     AALCFWVFTFFRYPKRNIPQENPNLIYSAADGKVVVVEKIKTDRFPEGEAIQISVFMS
                     IYDVHLNYVPISGTVTSVEHRNGLHIMAIKPKASLENEQSETVILTEKGQQIITRQIA
                     GAAARRVLTFVEKDQKVKAGDELGFIRFGSRVDHIIPVGSTIKVKPGEKVTGCKSIIA
                     ELN"
     gene            11468..>13161
                     /locus_tag="PKY63_09630"
     CDS             11468..>13161
                     /locus_tag="PKY63_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00914.1"
                     /translation="MAINPAKRITRIIKNGEPEHIWQFSRVGGVNRVNIVSGEDIALL
                     DTLDQKLWTALSCPVSGLEIDDKTLDLIDSDDDGRIRVPEIITAGKWITSVLKNPDEL
                     LEDRSKLPLDSIDDSTGEGKELLASAIQILKNLGKEGQTEISVEETSDTKGIFANTKF
                     NGDGIITADSTDDESLKSLIENIITCLGSKEDRNGNPGIDTELINNFFQHCEDYNTWQ
                     LVAKTNQKEILPFDGSTAAALDAFNAVKEKVDDYFLRCRLAEYDPASAEVLNAQLTQY
                     EAISPKNLAQNMIEIASLPVAKMNGTKNLPLIDSVNPAWSEPIAIFSELVVKPVFGPK
                     KELTLVEWQSLFQKFASYQQWLADKKGAEVEQLGAETIATILDNIHKESLLELVAEDL
                     KVEAEANNIIKVDRLVRYYRDLHTLLRNYVNFSDFYQPGIKGIFQAGELYIDQRCCDL
                     CIKVSDMPKHTKMAGGSGICLLYCDCVSRTGEKMTIVAALTDGDNDDITIGRNAIFYD
                     RKGQDWDATIVNIIDNPISIRQAFWSPYKKLSKFISKQVEKMAASKEKSMEEKNMG"
BASE COUNT         3733 a         3031 c         2797 g         3600 t
ORIGIN      
        1 cctactcctt attatccgta acttttccgt cggcgaacca gagaatgcgt gatggatatt
       61 tttccattac cagcatgtcg tgggtagcca tgagaatggc tttacccgtt tttttcaatt
      121 cgaccaatgt attcaatact tcctcagacg actgtggatc gaggtttccg gtgggttcgt
      181 ccaccagcag caattcggga tcgttgagca aagcgcgggc aataaccacc ttctgttgtt
      241 cgccgcccga cagctgatgc ggcattttat cttttttgtt ttcaagacca acgcctgcaa
      301 ggacgctgtc gatgcgggaa tccatcgctt cgcggtcacg ccagccagtg gcctgcagca
      361 caaacagcag attctctctc acattccggt cggtcagcag ctgaaaatcc tgaaagacca
      421 ttcccatttt ccgcctcagc aaagggattt cgcgcgtgcg gattttattc agatcgtatc
      481 caagcacagt cccgatggta ccttcgaaag gcagatcggc atacagcatt ttcatcacac
      541 tcgatttgcc cgagccggta cggccgagaa aatagacaaa ctctcccgat ccgatgctga
      601 atgaaacatt ggaaagaata aggttgtcgc catgacgtac cacggcattc tcaacagaga
      661 caagattttt tacttccctt acgggtttct gcggtgtttc aggcattccg gcaatttcgt
      721 ccatgaccat gttcattttt gcaaatgtac tattttttca ttgacgaaaa tccggacaag
      781 gcgcaacgca caagcctaaa gcttgttggg ccgaaggcgt caagaaagct gcgaagcacc
      841 accggatact ccggaggagt taaataataa tagcgttggg cgaagcccca gggacaaggt
      901 gtgcgaagct cctttgaatt ggaaagttat taaatcgatg taaaagcatg gtgcaagggc
      961 aagacgaatc tcgtcgctcc gctcctcaaa cagacatccg gatggttctt ctccgctgcg
     1021 cttcgaaaag aatatttccg ctgaccgcat attttctgat ccgaaaacca tatatggcga
     1081 caccgtgcgt tgagagtgaa ttttctcatc ctggtatcgt cacctgatgc gacatctggt
     1141 atttgggaaa actttggaaa acggcaccgg gcgtgcgttt gcataaatgt ttgtgaaggc
     1201 gcgcgcggcg cgcacagctc cgaaagccaa cctgcaaaaa agccactctg gtgagcggct
     1261 tttgaaatat ctcgaagaga ttttaaggat ttgttttgga tggtgcagta ttgcataaac
     1321 actaacaatt agtctattat atcttcatat ataaaagatg attcatcgat tttttccaaa
     1381 gaatttgtgt ttgaatagtc ttttttgtta acgatcagta ttcccccaac ccctccatta
     1441 cgaatatagt ttgaactaac tgcttgccca attccataaa aaactccact cgtatcaatt
     1501 cgaatggaca ctggattaat ttctttaagc ctatttattg tctcaataaa acttgtatca
     1561 ttgattagct gaaatgcgtt ttcggtttgt tcaccactac aactatcgca tgtggaattt
     1621 cttaataact ctaaatcatt ataatctaaa aaaaatgaaa tgtatccatc atttgattcc
     1681 cagcgacaat caacagtaat cccttcgtta aatttaagga taacttgttg aatttcatat
     1741 ttattcatca ccgtaataaa tgtttgtttt gattctaata acaatttctt atttttagaa
     1801 taacattttt tgaaatcatt agacttacta ttagtgcttt tcattgtgga aataggattt
     1861 gaacacaact gcattactgc acacaatata atacaaacaa ttgttctatt cataattatc
     1921 ttggtatttg aattatgccg taagttgaag cattaaataa accatagcta ataccccatc
     1981 gatatttcca ttgaccataa tcccagtaat gaatatcata tgaatcactt aacgtagaga
     2041 ttagatccac tcttatgaag tgagttccga aaaaattccc aattctttgt ccatttttaa
     2101 atgcatctga atttacatat aaaacaatat cgcttgaatt gagtaatttc gttaccaacc
     2161 ctcctttgtc aagattaaac catgttccgg catcaatacc atgaatacta acatcatatc
     2221 ccattttatt ccaaagcatc tttgctttcc atagatcacc accactccac ataggatctt
     2281 cttcatctcc tggatcataa tcattgtcaa aatgaattat gccacaatta agtccactag
     2341 cagctgtcat cgtgctcatt tgatcaatca cattgttaaa gtctgcatta ttactaaaag
     2401 tagaagaacc aattgcctcg taagtatcat cgggagtgga tctcgtagta taactgccat
     2461 taaaatacac aaactcccca gttttgtata aattaatcat tgcttttgac attcctgctg
     2521 gatctttttc ccaaacatat gataaacaag cagcagccca acaaaaattt gtgccatggc
     2581 cttgattaaa agccgttgga ttttgcaccc ttgatcttaa atcacttaga aatttttcag
     2641 gcgatacatt tttgtaaaaa tcactttggg tcaaatcttt tgatgcattt tcaataattg
     2701 aattggcgtc cgacttagtt gaggtagggg gatcgcctgg acctaacccc ataatatcta
     2761 catatagaat gggattattt gcagcgaaag catatggtga ttgccacgga tattttgctg
     2821 ataatgggtc aatagtaaca aatcttccta atcttgagtc ataatcacgg aattcaaagg
     2881 atatgaaatt cccagtccct ttcacctcat tgtcgttttc tttgccattg aatccgaacc
     2941 tatacgatga cgagttccag tttctttcgg gcatgatcat gccaaaggga taataatcct
     3001 gtgagctggt gatatcggcc agaaaataat ccacagttcc gtcgtttaca ttactgctaa
     3061 acggcagttt gtggtcggat attgtggcca gcacatttcc caggtggttg gtgagttcgt
     3121 attgcttatt gccggtggtg aatgagaacg atgaaccgct ccaggtgagt tcggtcattt
     3181 tctttccgat gttgttcaca ccaagacggc tgctgccgta gatgtcaaat tcgtcgagat
     3241 acacgtaatc gttggcatcg tcggtcatgg tgtatgtggc catgatgttg ccctgcgcat
     3301 cgcgcgagta gaaggtgtat ttcgctcccg tcgaagcgta ttctattttc attgtgcggt
     3361 tgcccatggg gtcgtagcgg aactccagat cgtttttgag gctggctgtt gtaaaatcaa
     3421 tataatttat ctttccgtac acggtccagg ttatgttgta ttcacgtcac aaacgcaact
     3481 ttctcaatat tcaaaattag aaaaattttc tttaatgcgc tgctaagttc cgtacgctgc
     3541 tttgtaccga ctctgcaagg acaccaagtg gatctggcag gtcgggagtg cagggcgcac
     3601 tgggagcggc gcaggatttt ttcgaagaac gtgaataaaa catatttagc cagaatttta
     3661 tcttgaacca attactattt tggttgtgga aattccaggc tgaatttcca ttccagcgaa
     3721 ttgatctttt ttgtaagatt tttcaaatcc gaaattattt catcaaaagc cggcggattt
     3781 tcttcataaa tcatttgttc aatcatgaca ttgtagtctg ctttccatgc ttccatcaac
     3841 tctgcgggag gtatcggatt tattgtctgt ggctgatgca aattgtagtt cacttcgcct
     3901 actcttgtaa aatggtatcg gtgatttaca atggtttcat acaaagccct gtctgcaagc
     3961 gctttttcag caaaacttgt tttggataat ttcaccacat cgtacaaatg gcgactgagc
     4021 cggttaactc ttcttttttc agctggccgg tgaaattctt catgtaaaag aaaaattttc
     4081 tcaagaaaag ttctttccgg attaacggtt ggaataatgg caggagactg tataaaaccg
     4141 gcgtcaggga atacttcgtc aaggtgtgga atgatgcttc gcattgtgaa aggttcgcgt
     4201 aaagaacgac aactgatctc tatctgaaca gcaggctgca ggtagccggg tgcttcaatc
     4261 acatttggat aataaatgga gattttacga tccaggtcgc tttgcttttc ctttaccagt
     4321 tccagccgca cgttgtcata ccctttgctg gcaaactccc cgattaattc cggataaaaa
     4381 acattatcaa cgtacaatcc tgtttctttg cggagtttgc ctcgttcatt tttactgagc
     4441 tctccatcaa agccaagaaa agaccgatcc agaaccagat caatatcttc ggaaaaccgg
     4501 tcaataattt tccacccctt gctaagcgag gtgccgcctt taaacacgag gtgtgccgac
     4561 acatccatcc cgaaaacaac agacaatgtt tgcaccaccc accagtcctt ttccgcagca
     4621 aacgctgaca gaccggtttt ttctgctgtt tcagcataag cccttctctt tgtttcgtca
     4681 gggactgcta tccagggatt catgattttg atttttttat tgccttccgc attattatcc
     4741 gtatccattc aggagccaac cttatatcat gttcaagtct atagggatct tcttttttca
     4801 aatgtttcag aacaactttt atttccgctt cggtgacatt atccttcccg atgtctttaa
     4861 gagcctgaat aaccacactg ctgattttcc caattgcagc cagatttctg ggcgttgtct
     4921 ttttaaaaat aatttttcgc ttgccaatgg aaattcgtct cgctgcgccg tcagtaagat
     4981 aaacaacatt tattgggacc tgcgtggaaa ggcccaatgc attcaatgca gcagctccag
     5041 taataataat ccggcttttg tctctgcggc tgatggcttc agcaatgact tcagtgcctg
     5101 gcgttatcgt tccgagaatg cgatcgcttt ccaggcgcgc ataaattcca cgcgcaacgc
     5161 gcgaaatact cccctgcccg accaaacgct ccaatgcttt ggctacagct tttgcactac
     5221 cgaaagacag aaaatcttcg gtaaaaaaaa gcgtacccct cttcgccttt ttaatttttg
     5281 caagtatcat gtcatcagca cttaacatgg cgtttgatta ataaatatgt cgcaaatata
     5341 gtaaataatt gcgacattat taccgcagta caaagttgaa agatttcatt ttaataattc
     5401 agatttttcg catgaactga attcaaatac aacatatgaa tctcactcta ctttacagca
     5461 atctgaattt ttcgagagac gcacaccttg agtaaagata gaatattttc tcaaagtgtg
     5521 cgtaatgtta aaaatgggtc gcatttatcc gaaatggttt catgccctca gaaacatgaa
     5581 accacgtcgg aagggggttt gagtagtgtg ggtgcggaat atagcgtccc aaaaatgaaa
     5641 tatttttatt gaaatttgct ctgagcgcgc gtttagataa tactatgcaa aaccgcgcgc
     5701 tgcacccatc ctccctgacg atatgccatc gtccgcacga agaaaggata tggcatcgtc
     5761 ggcagggaat tctcaaagcc gctcccaaag ccagaagccg aaacttaccg accctgccag
     5821 gactggggcg caggctacaa gccgcatgca tagtccacag ctccgtgtca gagcaccgac
     5881 gcttcggtcg gcacctactg ctgaaagctg agtcgtaacg attgtaacga ttcccgcttc
     5941 cagcgtgata aattccacgc gtaacgcgta acgtctgtaa cgtcaaatca acaaatcatc
     6001 gcgatagcac atggcaattt cgtggtggaa aaatgtgcgt tccttgccgt cgggcatttt
     6061 cagacgcagc tgtccatttg gttctgagcc aaggatgcgg catttcagaa tatcgtcgcc
     6121 aagcttgaaa ttctgatcgg tgccaacacc aaacaggcgg ccgtcgtagg accagaaagt
     6181 tttttcggcg ccgagtgcca gaaaattctc ataactggtg agaaaagccc cggtcagttt
     6241 attgatttcg gtttcaatat cccgtttttt accatccatc atttcgagag aaacagcggc
     6301 ctgcagatcg cctgcaaaat gaatctgatt cacattcacg ccaataccaa tcacagtggc
     6361 aagaatatca tttcctttta gtgaattttc gatgagaatg ccggcaattt tgcggctatc
     6421 aaccataatg tcgttgggcc attttatgga gacttcggct ccgggcaatt cggccgaaac
     6481 ataattgtgg gctgccaccg caataatctg gctaagcaca aactgatcag ccactttcac
     6541 tgccggattt ttcaacaaga tactcataag aatattttcg ccagtttcag attcccatgt
     6601 atggtctccc tgcccgcggc cggccgactg aaacgccgca ctgaaaatat gcagctcatt
     6661 attcaaaact tcattaacct ggctcatagc ccactgattg gtggattcta caacttcgtt
     6721 tctgtgaaat atagttttca tagctttgat tttttaggag agttatcacc aatggtttgt
     6781 tataatgtca aattgagtaa ctttgtacaa atttacaaag attaaatggt aaaaactgtt
     6841 aaagacccgc ttgtatcgaa attaaccaaa gcgctcaaat caaaaaaagc gcaaaacatc
     6901 gtcgttctcg acatgcgtga aaccggacaa aatgtctgcg atttttttgt gatttgtcat
     6961 ggagtttccg gcgttcaaac gcagggtatt gccgaaaatg ccatccggga actaaggaaa
     7021 gaagagggtt tcaagcccat tcaagtggaa ggactgtcca atgccgaatg gatactggtg
     7081 gactacggca atgttgtggc acacattttc agagaagaca tgcgcgaatt tttccggctt
     7141 gaggatttat gggccgatgc aaaaatcacc acaatttaaa taacagaacc acagagcttt
     7201 aatatgaaag ataagcccaa agacaaccct aatcaaggtc cgaaaaagaa attcaatttc
     7261 tactggatat atgccataat ggcaatagca tttttcgcca tgtattttta tggatccgaa
     7321 ccacaggagc ccaaagaaat catgtggcaa cgtttcgaaa acgaaatgct ggccaaacat
     7381 cacattgaaa aaatcgtggt ggtgaacaag gaatttgccg aaatccacat taagaaagac
     7441 attgtaacat ccgacagcag ttacaaagac gcagtgaaag gcaaagaaaa catcgggcct
     7501 cactacacca tgaaattcct gtcgcccgaa agtttccaga cgcaactggc agcggcagaa
     7561 gaccgcattt ttgccaaaga tacggttgga aaaacacccc aacaagttct tgaacttgaa
     7621 aaaacacgtc atgtaaatgt tgttcccgaa gagcgcacca actggggcag caccattctg
     7681 gtgaactgga ttctgcccat tgccatcgtg cttggcatct ggtttctgtt catgcgcatg
     7741 atgaaccggg gcagcggcgg cggtcagatt ttcaatgtcg gaaaatcaaa agctcaattg
     7801 ttcgacaggg atactcaggt ctccatcaca ttcaaggatg tggccggact agaagaagcc
     7861 aaagttgaag tggtggaagt agtggatttt cttaaaaatc cgaaaaaata caccagtctt
     7921 ggtggaaaaa tacccaaagg cgtgttgctg gtaggccctc cgggcaccgg aaaaacattg
     7981 ctggccaaag cagtagccgg tgaagccaaa gttcccttct tctccatcag cggctccgat
     8041 tttgtcgaaa tgtttgtcgg tgtaggcgct tcgcgggtac gcgatctgtt ccgtcaagcc
     8101 aaagaaaaag caccctgtat tgttttcatc gatgaaattg atgccattgg gcgtgcccgt
     8161 ggccgtgcca atatcaccgg tgccaacgat gagcgcgaaa atacgctcaa tcagctgctt
     8221 accgaaatgg acggcttctc aaccaatgcc ggcgtcatca ttctggcagc taccaaccgc
     8281 gccgatattc tcgacaaagc cctcatgcgt gccggacgat tcgaccgcct gatttatgtc
     8341 gaactgcccg acctgcacga acgcgaagct attttcaatg tccacattaa accgctcaag
     8401 ctgtccgaag acgttaaatc ggattttctg gccaaacaga cccccggatt cagcggagct
     8461 gatattgcca atgtctgtaa tgaagccgcg ctgatagcag cccgtaatgg gaaaaaagcc
     8521 attgaaaaac aagacttcct cgatgccata gaccgcatta ttgccggact tgaaaaacga
     8581 aataaaatta tttcggttga tgagaaaaaa accattgcct atcacgaagc cggacatgct
     8641 tcggtaagct ggttactcga acacgcccat ccgctggtga aagtaaccat tgttccacgc
     8701 ggaaaagcac ttggagcggc atggtatctt cccgaagaac gttccatcac cacatggaat
     8761 cagatttacg atgaaatgac ctcggctttg ggtggacgtg catcggaaga ggtgaacttc
     8821 aacgaagtct ccaccggcgc actcaacgat ctggaaaagg tgaccaagca ggcctacgca
     8881 tcggtggcgt atttcggact gagcaagaaa ctcggaaaca tcagcttttt tgattcatcg
     8941 ggatcacagg aattctattt taccaaaccc tactcggaga aaactgccga agtaattgat
     9001 tccgaagtac ataacattgt tgaaagtgct taccaacgtg caaaggacct gctgattgcc
     9061 aaccgcgaaa aagtgtcaca actcgccaaa ctattacttg aaaaagaagt aattttccgc
     9121 gaagatgtgg aacatatttt cggaccacgt ccctgggccg acaaggaaac aacaaagcat
     9181 gtcgcaccaa aagtcattga aacatcttct gaagaaactc ctgaggaaaa gcctgaggat
     9241 aacaccaaag ctgatacaaa agaataacaa tctgccggca tgaagcagtt cgaacatatt
     9301 gaattacgcg aaaaggtgct caaacgcatc cgcaatgcgc tgatcaataa aaccgaactc
     9361 aatgccgaac agctgaatgt tgaaatcgcc gcattcaaac cttttcagca ggccattccg
     9421 tttgaatttg caaaaaactt caccgatgca ggcggctttt tctattattc cgaaaaccgg
     9481 caggaactca aaattgccat caacacactt ctgaatgaaa agggctatat gagcgtgtat
     9541 tgcagcaacg acgatttgtc gtcgcttgta aatacaggcg tcactgccgt tctgaccgag
     9601 ctgggagatc tgccggctac tccggtggta atcaccgaat gtgagtttct ctgcgcacgc
     9661 accggaagta ttctgctcag tagttttcta agctgcggtc gccggggcat cgccaccaac
     9721 gacgctttga ttgtggtggc aaaaatcgat cagattcttc ctgatattca ggacgccatt
     9781 caggccatga ctgaaaaata ctccgaagag tttcccagcc agctgacttt tgtgactggt
     9841 ccaagctgca ccaatgccat tgaaaataat tttatccgcg gtgcacacgg ttctaaggaa
     9901 gtttacgttt ttcttcatta atcaactaaa actccgatta tgaagaattt ccttgtccgc
     9961 tccgcttctg ctgttgttta tgcaggtctg ctcatcgccg ggagtctttg gtggtcaccg
    10021 ctgctctacc tcactctgac ggcctttcta atcatcggcc tgctggaatt gaaaaagctg
    10081 ttgcaaacgg atattcacag gattttgctt gtcatctggt ccattatcgg tgtgctgttc
    10141 atgctgataa cggttgcatt gtttgaccga acgctaattg acaatgattt tgacagtgaa
    10201 gcattgacac cgctgctttt cttttctcca tttttacttg cctttataat attaataatc
    10261 agcactatat cgcgcaaacc ggcgcaagga cttttgtcat ttttttcaat ttccattgtt
    10321 tatctgctga tccctttcat gttcatgata ggaatacaga atgcggtttt tgccgattcg
    10381 cagccctggc tgctggttct gctggctgcc atctggatca atgacagcat ggcctatatc
    10441 actggcagtc tgattggaaa acacaagctg atcgagcgca tttcacccgg caaaacctgg
    10501 gaaggatttt tgggcggact aatatttaca atcgctaccc tattgattat caatcagata
    10561 ttttttgact ttgatgccat ttcactcgca atcattgctt tggtgattgt gatgagcggc
    10621 accatcggcg atctgctcga atccaagctc aaacgcgaag ccggtgtaaa agactcgggc
    10681 aacatcattc caggccacgg cggaatcctc gaccggatcg acagcttgct ggtggcggct
    10741 ccttttactg gtatagcatt gttaatcaac gaaataatta tacattggca ctaatgagaa
    10801 cacatcctgc aggaaaagat gtaataaatt gggttgttac aattcttggc accattgccc
    10861 tgatggtcat catgtttgtg ccttttaagt tattctatgt tatttgtggt gttttgttta
    10921 ttctggctgc gctgtgtttc tgggtattca cttttttcag atatcccaag cggaatattc
    10981 cacaggaaaa tccaaacctg atttattcgg ccgccgacgg caaagtggtg gtggttgaaa
    11041 aaatcaaaac cgaccgtttt ccggaaggcg aagccattca gatttcggtg tttatgagca
    11101 tctacgatgt acatctgaac tatgttccca tcagcggaac cgttacttca gttgaacacc
    11161 gtaacgggct gcatatcatg gctataaagc caaaagcttc gctcgaaaat gagcaatccg
    11221 aaactgttat tctgaccgaa aaaggacaac agatcatcac ccgccagatt gccggagctg
    11281 ctgcccgaag ggtgctgact tttgttgaaa aagaccagaa agtgaaggcc ggcgacgagc
    11341 tcggattcat ccgttttggc agccgggtcg atcatatcat tccggtcggt tcaaccataa
    11401 aagtgaaacc gggcgaaaaa gtgaccggat gcaaaagtat tattgcagaa cttaactaat
    11461 tgtttcaatg gccataaatc cagcaaaacg aatcaccaga atcatcaaaa acggtgaacc
    11521 tgaacatatc tggcaatttt cacgtgtagg cggtgtcaac cgcgtcaata ttgtttcggg
    11581 cgaagacatt gccctgctcg ataccctcga tcagaagcta tggacggcgc tgagctgccc
    11641 ggtctctggc cttgaaatcg atgacaaaac actcgacctg attgactccg acgatgatgg
    11701 acgcatacgc gtgccggaaa taattacagc cggcaaatgg attacatctg tgctgaaaaa
    11761 tcccgatgaa ctgctcgaag accgcagcaa actaccgctc gactccatcg acgattcaac
    11821 cggcgaagga aaagaattgc tggcctcagc cattcagatt ctgaaaaacc tgggcaagga
    11881 aggtcagact gaaatcagcg ttgaagaaac ctcggatacc aagggcattt ttgccaatac
    11941 aaaattcaat ggcgacggca tcattactgc cgattcaacc gatgacgaaa gtctgaagtc
    12001 actgatcgaa aacatcatca cctgtctggg ctccaaagaa gaccgtaatg gaaatccggg
    12061 aattgacaca gagctgataa acaatttctt tcagcattgc gaggattaca acacctggca
    12121 attggtggca aaaaccaacc aaaaagaaat tcttccattt gatggcagca ctgctgcggc
    12181 actcgatgca ttcaatgccg taaaggaaaa agtggatgat tattttctgc gttgcagact
    12241 ggcggaatac gatccggcct cagccgaagt gctcaatgcc caattgactc agtacgaagc
    12301 catcagccct aaaaatctgg cgcagaacat gattgaaatt gcctctttgc ctgtagccaa
    12361 aatgaacggc acgaaaaatc ttccattaat tgatagtgtc aatcccgcat ggagtgagcc
    12421 aatagcaatt ttcagcgagc tggtggtcaa acccgttttt ggtccaaaaa aagaactgac
    12481 tcttgttgaa tggcagtcac tgtttcagaa atttgcatcg tatcaacaat ggctggctga
    12541 taaaaaaggg gctgaagttg agcaactcgg tgctgaaacc attgcaacca ttctggataa
    12601 catccataaa gaatcgcttc tggagctggt tgcagaggat ctgaaagtgg aagccgaagc
    12661 caacaacatc attaaagttg atcggttggt gcgctactac cgtgatctgc acacactttt
    12721 gcgcaattat gtcaatttca gcgattttta tcaacccggc atcaaaggaa tttttcaagc
    12781 cggcgaactt tacattgatc agcgctgctg tgacctttgc attaaagtgt ccgatatgcc
    12841 caaacacacc aaaatggccg gcggcagcgg tatctgcctg ctctactgcg attgtgtttc
    12901 gcgcaccggt gaaaaaatga ccattgtggc agcactcacc gacggcgaca acgacgatat
    12961 caccattgga cgcaacgcca ttttctacga tcgcaaaggc caggactggg atgccaccat
    13021 tgtaaatatc atcgataatc ccatcagcat ccgccaggca ttctggtcgc cctacaaaaa
    13081 gctgtcgaaa ttcatttcga aacaggttga aaaaatggcg gcatcgaaag aaaaatcgat
    13141 ggaagaaaaa aacatgggca a
//
LOCUS       DAOPKE010000077        13135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_4974_length_13135_cov_10.809021, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000077 DAOPKE010000000
VERSION     DAOPKE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_13135_cov_10.809021"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2446)
                     /locus_tag="PKY63_09635"
     CDS             complement(<1..2446)
                     /locus_tag="PKY63_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00915.1"
                     /translation="MKKLLLLFILLAFSGFAFSQNLVINPGFETVNTGALQCDMYSDA
                     EFDAAITTWTSPTSGSSDLYHTSLATSCRSCPNCTGTACIGNQTPHGGSAMVGIITHL
                     SSSSFCSPNYREYIQGTLSSALVVGQEYCVEFWVSSADDAPYATNNIGIKFYTTAMNN
                     TSLCPIGVAPDYNYPTPITNTSGWTLISYSFTATAAYRYFIIGNFYNNAGTTIVANGG
                     SESTYAYYFIDDVSVELCSSNPVVTVNSPTICSGQSATLTAAGANTYSWSTGATGASI
                     TVSPTTTTNYTVTGTSAGGTGSAVATVTVNPTPTVNVSASPTSVCPGVASTLTASGAA
                     SYSWSTVASGSSISVTPSTTTTYTVTGSSSAGCTNSSTVTVVTNTAPTVTVAGASICQ
                     GASATLTASGAAVYTWNTGATTASITVSPASTTIYTVTGTNAVGCTGSATATLTVNPI
                     PNVSVSASPASVCPGNASALTASGAATYSWSTGATGGSISVSPASTTTYSVTGTSSAN
                     CSATGSVTVTTLPGPVVSATGAVICAGNAAAISASGASNYTWSDGSTGASVSVSPSTT
                     TVYTVSGTDGSGCTGVATATVTVNPLPVITINSPAICSGATADLTATGAATYLWNTGS
                     TANPLQVTPSSTTSYTVTGSDLNNCSNTASATVIVNASPALTLSSTDDYCNLSDGTAT
                     VVAAGGLAPYSYAWNTSPAQTSDVATGLAAGQYEVTVSDANGCQKSGIVTVFALAGFT
                     LNSDSEVEHCMHGDGSATITALNAVNPLTYSWSHNASLNSATANNLAAGTYSVTASDG
                     GCSQVISVTVGSYSGPV"
     gene            complement(2443..2862)
                     /locus_tag="PKY63_09640"
     CDS             complement(2443..2862)
                     /locus_tag="PKY63_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00916.1"
                     /translation="MKTFFVSAFYAGLMLLGCQLSAQVKVHVNDCSWISVSVVDGPDG
                     LPIENYEIGPGPDGYYLFYSDETESVLIMEGELENGQRQGEWKFYNNNGTLYCRCHYV
                     NGLLNGLYELFNHDGLVSESIEMLNDLPVENHSKTKL"
     gene            complement(3059..4111)
                     /locus_tag="PKY63_09645"
     CDS             complement(3059..4111)
                     /locus_tag="PKY63_09645"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPF00917.1"
                     /translation="MKKIFISLATSFLMITAGQAQIVTNLSSDFNALVDSFVMTGVSV
                     SNVQYTGDSSSIGSFSDDNTSNLDIDHGIILTTGTLTGSPAIGSPVSNFASTINSQPG
                     DSLLDLLIPGYLTYDASVLEFDLVPVGSVLQFEFVFASEEYPEWVGSSFNDVFGFFIS
                     GPDTAGNYYNNMNVALIPGTITPVSINNVNSALNAGYFIDNELLGDSNFVFDGYTTTI
                     TVTVQVVPMSSYHLKMAIADAGDGFYDSGILLECPSLRSYMPTDIETANENTNVYPNP
                     MRPNSVLTLNVLQAGPVRICIFDATGSVISTFNENVDTGTMQVPVGHLLNKLPSGVYY
                     ASILMADQQSVIKLVR"
     gene            complement(4220..5137)
                     /locus_tag="PKY63_09650"
     CDS             complement(4220..5137)
                     /locus_tag="PKY63_09650"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPF00918.1"
                     /translation="MTFNLTVIGSGSGAPLPGRFTSAQVLNVHERFVLIDCGEGTQIN
                     LRRFGINFNRIGLILISHLHGDHYLGLPGLLNTFHLLGRQKELHIAGHRKLKEVLDFN
                     TALSGFEPSYPIYFHDIDALEPGKELDFEEYKVSTFPLTHRIPASGFSVTERPRTAKV
                     RKEFIEKYQPGYEQVRAVLRGEPFFDVNGNEVPIDEMLMKPAPPRSFAYVSDTVFDKS
                     IIPFIQGADLLYHETTYDKSLQNSAAEKMHSTSVDAATIALEANVKKLLIGHFSSRYR
                     TPDLLLDEARRIFPETVAATDGLIISLQE"
     gene            complement(5138..5887)
                     /locus_tag="PKY63_09655"
     CDS             complement(5138..5887)
                     /locus_tag="PKY63_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF00919.1"
                     /translation="MKRIFTLFAGLFLAAALNAQPCVPDAQYTTPGIFPDTLINLPPV
                     TIGVAYTGVITAVVPADTSYMGFSATIDSINVIEIVGLPSGFTWSGAPTGVGAPVSIP
                     GGSNGCIAIIGTATAGQTGVYPLKIRVIAYGEAMSMPMSLPNTVNGYKLVIGNVGFGE
                     NPYYSFGVTDMYPNPAADVANIVVTNNETSIVAVTISDMVGRTVSASNHRVNAGENTI
                     ALNVAALPEGVYFYTVAKGTQTVTRKLIISR"
     gene            complement(6182..8041)
                     /gene="rpsA"
                     /locus_tag="PKY63_09660"
     CDS             complement(6182..8041)
                     /gene="rpsA"
                     /locus_tag="PKY63_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPF00920.1"
                     /translation="MSDELNKTENTNLEEQAPALETTAATTAKVEEFNWDAIGKKDQI
                     YSAAEREKLEGLYVKTLSQITEKEVIDGTVIAKTDREIFVNIGFKSDGVIAANELRYN
                     PDLKIGDVIEVYVESQEDGTGQLILSHKKARLLKAWDRINVLYETGEVIKGEVKCRTK
                     GGLIVDVFGIEAFLPGSQIDVKPIRDYDVFVGKIMDFKIVKVNNEFKNVVVSHKALIE
                     DELEAQKAEIIAKLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRINRPEE
                     IVELDQKLNVVILDFDESKKRIALGLKQLTAHPWDSLDTELKVGDKVKGRVVVLADYG
                     AFVEIATGVEGLIHVSEMSWSQHLRSAQDFLKVGDEVEAIILSLDRDERKMSLGIKQL
                     IPDPWVNIKDRYPINSRHKATVRNFTNFGVFVELEEGVDGLIHISDLSWSKKIKHPAE
                     FTKIGDEIEVVVLDVDVENRRLSLGHKQLEENPWDVFETIFSVDSIHKGTIIEKNDKG
                     AVVSLPYGVEGYAPNRHLQKEDGSKAEMDEALDFKVIEFSKESRKIVVSHSKIFQEVK
                     EDKKREPSEKRQDDSFSKAKSNAEKETLGDLDIFANLKEKKAKGKKADGSDEE"
     gene            complement(8473..9174)
                     /locus_tag="PKY63_09665"
     CDS             complement(8473..9174)
                     /locus_tag="PKY63_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF00921.1"
                     /translation="MKAFEPNLKDLDIDNLTEFLTDFITEKRLERIKEVASKRTQHFA
                     VALENLFQGHNASAVLRTCDCFGIQNVFAIENVNKFQPNEEIALGAEKWLTINHFNEE
                     NSIEKCYTELRRQGYRIAATTPHGNPVPVDEMDVSKPLALVFGTEKEGLSEKAIELAD
                     FTVALPMYGFTESFNISVSAALFLQKVIDKSIAQKVDRFIGIKEQQELIAQWILKTVS
                     DPEGLMKKYLEIHKA"
     gene            complement(9468..10502)
                     /locus_tag="PKY63_09670"
     CDS             complement(9468..10502)
                     /locus_tag="PKY63_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HPF00922.1"
                     /translation="MLFPFDPDKNFSKNNGIFGLPKDENNAALLIIPVPWDVTTSYRE
                     GTAYAPEAILEASYQIDLFDTQLPDEWKKGIFYAPFEDWIMDLNSFERRKSKTIIDHL
                     ENSDEPLPDRLKRAQDEVNEACVQMNSYVSAKVNKCLAFGKIPALLGGEHSISFSAIA
                     EVAKKFPGLGVLQFDAHADLRQHYLDFQFSHASVMRNVLENVPEMGKLVQVGVRDLCN
                     DEAHFAASSDKIETFYNTIMTERMFEGETWKTITDEIVAALPQQVYITFDIDAFKPSL
                     CPHTGTPVPEGLTFEQAVYVVKKLVKSGRHIVGFDLVEVAPDHENQWDETVGCRLLYQ
                     LCIHALASQK"
     gene            10779..11885
                     /locus_tag="PKY63_09675"
     CDS             10779..11885
                     /locus_tag="PKY63_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPF00923.1"
                     /translation="MGLLGKIKGEFIDIIQWLDPTNDTIIHRFERYNNQIKYGAKLTV
                     RESQMAVFINEGQIADVFGPGMYTLETQNVPILSTLKGWKYGFNSPFLAEVYFINTKN
                     FTDRKWGTKNPIMMRDAEFGMVRLRAFGNYQFRVGDPAKFIKEIAGTDGAFTTDEIEG
                     QIKDVIMTRFTDYVAEAKIPVLDLASNYNELSKGIQEHIKPELDEYGLNLTKFLIENI
                     SLPPEVEAMIDKRSSMGIVGDLNQYTKFQTANAIEEAAKNPGGDAGAGMGMGMGFAMA
                     NQMSQTLNQPNQQQQQQQQAPPPPLIQYFVAVNGQQSGPFPMPTLQQMVQQGSLTKET
                     LVWTNGMANWEPAGQVQALAGIFPVTPPPMPPQM"
     gene            11960..13072
                     /locus_tag="PKY63_09680"
     CDS             11960..13072
                     /locus_tag="PKY63_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00924.1"
                     /translation="MSDLSPEFEGKHAETLETKCTNCGAVLNFAPGTNHLKCQYCGAE
                     NEIFPAEAPIEELDFEQYLQDGAMNADTVQVVTVKCSGCGASTTFQPNIASDTCAFCG
                     MPIVVNESSTATVIKPKSLLPFKITQKEAFECFLKWLRKLWFAPSALKKFVNQPDKLR
                     GMYIPFWTYDSDTTTRYTGARGTHYYVTETYTSNGKTETRQVQKTRWTPVSGTVFKFF
                     DDILVVASTSLPMKYVNKLEPWDFENLAPFDDRYLSGFRTETYQIDLKNGFELAKKFM
                     EPGIRALIRQDIGGDEQQIYSVNTQYSNIKFKHLLLPIWISAYRFKNKVYRFLVNGRT
                     GEVQGERPYSFWKIFFFTLGCIVVIAGLLWAFGVFK"
BASE COUNT         3690 a         2839 c         2920 g         3686 t
ORIGIN      
        1 ctaccgggcc gctgtatgag ccaaccgtaa cgctaattac ctgtgagcat cctccgtcgc
       61 tggctgtaac ggaataagtg ccggctgcaa gattatttgc tgtcgctgag tttaaagacg
      121 cattgtgtga ccatgaatag gtgaggggat tcaccgcatt gagtgcagtt atggtggcag
      181 aaccatcacc atgcatacaa tgctcgactt cagaatccga attcagtgta aaacccgcca
      241 gagcaaagac ggttacaatc cccgatttct gacagccatt ggcatccgat actgtaactt
      301 cgtattgacc ggctgcaagt cctgtggcta catcgcttgt ttgtgctgga gatgtgttcc
      361 aggcataaga atatggagcc agaccgcctg cagctaccac tgttgcagtt ccatccgaca
      421 aattgcaata atcatctgtc gatgaaagag tcagcgcagg agatgcattg acgataacgg
      481 tggccgaagc cgtattgctg caattgttca aatcgctgcc tgttaccgta taactggttg
      541 ttgaggaagg tgttacctgt aaaggatttg ctgtactgcc ggtattccac aaataagtag
      601 cggcaccggt agccgtcaga tcggcagtag ctcctgaaca aattgcagga ctgttgattg
      661 tgattacagg caatggattg accgttactg ttgctgttgc cacacccgta catccgcttc
      721 catcggtacc tgaaactgta taaacagttg ttgttgaagg acttacagaa acagaagccc
      781 ctgtgctgcc atcactccag gtataattgc ttgctcccga tgctgaaata gctgccgcat
      841 tgcctgcaca gatcacagct ccggtagcag atactactgg ccccggaagt gtggtaacag
      901 ttacgctgcc cgttgccgaa cagttggccg acgacgttcc agtaacactg taagtagttg
      961 ttgaagcagg cgaaacgctg atagatccac ctgttgcgcc cgtactccac gaataggttg
     1021 ccgcacccga agctgtcagc gcagatgcat tgcccgggca gaccgatgca ggcgaagcac
     1081 taactgatac attgggaata gggtttacag taagtgtggc cgtagcagat ccggtacaac
     1141 caacagcatt ggtaccggta accgtgtaaa tggttgttga tgcgggagaa actgttatcg
     1201 aagccgtagt ggcacctgta ttccatgtat aaacagccgc acccgatgcc gtaagagtcg
     1261 ccgatgcacc ctgacagatg ctggcacccg ctacagtaac agtaggagcg gtattggtaa
     1321 caacagttac cgttgaagaa tttgtacatc cggccgaact gctgcccgtt acagtataag
     1381 ttgttgttgt tgagggggta acagaaatac tgcttccact tgcaacagta ctccagctat
     1441 aagaggcggc acccgatgct gtaagcgtgg aagccactcc cggacagacc gacgtaggcg
     1501 acgcagacac attaaccgta ggtgtcggat taacagtaac cgtagccacc gccgaacccg
     1561 ttccaccagc tgaagtaccc gttaccgtat aattggtggt tgtggtcgga cttaccgtta
     1621 ttgaagctcc ggtagcaccg gtgctccagc tataggtatt ggccccagca gctgtcaatg
     1681 tagcactctg acccgaacaa atagtcggac tgttgacagt aacaaccgga ttgcttgaac
     1741 acaactcaac actgacatca tcgatgaaat aataagcata ggttgattca gaaccaccat
     1801 tggcaacaat cgttgtaccc gcattattgt aaaagttacc tataataaaa taccgataag
     1861 ccgctgttgc agtaaaagaa taagaaatca gagtccatcc ggaagtgttt gtgattggtg
     1921 ttggataatt ataatccgga gcaaccccaa taggacacag agaggtatta ttcattgctg
     1981 tggtatagaa tttaatacca atattgttgg tagcatacgg agcatcatcg gcactggaaa
     2041 cccagaattc gacacaatat tcctgaccaa caaccaaagc cgacgacaaa gtaccctgta
     2101 tatattctcg ataattggga ctgcaaaagc tggaactgga aagatgagta ataataccaa
     2161 ccatagcact tccgccatgg ggtgtctggt tgccaataca ggcggttcca gtacaattcg
     2221 gacagctcct gcatgaagta gccaatgacg tatgatacaa atccgaagac cccgaagtag
     2281 gtgatgtcca ggtagttatt gctgcatcaa attcagcatc agaatacata tcgcactgaa
     2341 gagctcccgt attgactgtt tcaaaaccag ggttgattac cagattctgg ctgaaagcaa
     2401 aaccagaaaa tgccaataaa atgaaaagaa gtaacagttt tttcatagtt ttgtttttga
     2461 atggttttca acaggcaaat cgttcagcat ttcaatgctt tcagatacaa gtccgtcgtg
     2521 attgaaaagt tcgtacaatc cattcagcaa gccattaaca taatggcagc gacaatacaa
     2581 agtaccattg ttgttgtaaa atttccattc accttgccgc tggccgtttt caagctcgcc
     2641 ttccatgatg agtacagatt ctgtttcatc agaataaaac agataataac cgtcggggcc
     2701 aggtccaatc tcataatttt caattggtag tccgtcgggg ccatctacca ccgacactga
     2761 aatccatgaa caatcgttca catgcacttt aacctgtgcc gacagctggc agccaagcaa
     2821 catcaaacca gcataaaaag ccgatacgaa aaatgttttc atttgtaacc cccttatttt
     2881 tggccgaaaa atcaatcctt ttgactacaa aaagatagat gatgtaaaat tagtgtttct
     2941 tttttttgcg ccgcatattt attgaaattt attattcatg cctggttgtg aaaattcatc
     3001 cacaaacact gatttttttt agctgattat ttatttcagc acctgaacca tgtaaagact
     3061 aacgcaccag tttaataacc gattgctgat cagccatcag aatgctggca taataaaccc
     3121 ccgacggaag cttgtttaaa agatgtccaa caggtacttg catagttccg gtatcgacat
     3181 tttcattaaa agtactgatt acagaacccg ttgcatcgaa aatgcaaata cgtaccggtc
     3241 cggcttgcag cacattcagg gttagaaccg aattcggccg catcgggttg gggtagacgt
     3301 tggtattttc attggccgtt tcgatgtcgg ttggcatata actgcgcaaa ctcggacatt
     3361 cgagtaaaat tcctgaatcg tagaagccat cgccggcatc ggctatagcc attttaagat
     3421 ggtagcttga catcggaacg acctgtaccg taacggtaat ggttgtcgtg tatccgtcaa
     3481 aaacgaaatt cgaatctcca agcaattcat tatcaataaa atatccggca tttaaggcac
     3541 tgtttacatt attgatcgaa accggagtaa ttgttccggg tatcaaagct acattcatgt
     3601 tgttgtaata atttccggca gtatcaggac cagagataaa gaaaccaaag acatcgttga
     3661 acgaagaacc gacccattcg ggatactctt cagatgcaaa aacaaactcg aactgaagta
     3721 ctgatccaac cggtaccagg tcaaattcaa gcacactggc atcgtaggtt aaatagccgg
     3781 gaatcagcag atcgagcaag ctgtcgccag gctgtgagtt aatggtagag gcaaaattac
     3841 ttacaggtga gccgattgcc ggagaaccag ttagtgtgcc tgttgtcaga ataatgccgt
     3901 ggtcaatgtc cagattggat gtattgtcat ccgaaaagga gccgatggat gaagaatcgc
     3961 cggtgtattg aacgtttgaa accgatacac cggtcatgac aaaactatcg acaagcgcat
     4021 taaaatccga actcagattg gttacaatct gcgcctgtcc ggcggttatc atcaaaaaag
     4081 acgttgcaag gctaataaat atttttttca tgatgaatta ggtaaatttg acactcagtt
     4141 aatgatgaaa cacgcttaat ctttttgaaa acatacaaag ataaacaaaa tagtaaatat
     4201 tttgttaccg gaaactgatt tattcctgca aagaaatgat cagtccatca gttgcagcaa
     4261 ccgtttcagg aaatattctc cgggcctcat ccagcaacag atccggggtg cggtagcgtg
     4321 atgaaaaatg tccgatcaaa agtttcttca cattggcttc aagcgcaata gtggcggcat
     4381 caaccgaagt ggaatgcatt ttttcggctg cggaattctg caacgattta tcataagtcg
     4441 tttcgtgata cagtaaatcg gccccctgaa taaacggaat aatacttttg tcgaaaacag
     4501 tatcagatac ataggcaaaa gagcggggtg gagccggttt cataagcatt tcatcgatgg
     4561 gaacttcatt gccattaaca tcaaaaaatg gctcgccgcg caatacggca cgaacctgct
     4621 cgtatccggg ctggtatttt tcaataaatt cttttctgac tttagctgtc cgcggacgct
     4681 ctgttacact gaatcccgat gccggaatgc ggtgtgtgag cgggaaggta cttactttgt
     4741 attcttcgaa atctaattct ttgcctggtt ccaaagcatc gatatcatga aaataaatcg
     4801 gatacgatgg ctcaaatccc gacagcgccg tattgaaatc aaggacttcc tttagtttgc
     4861 ggtggccggc aatatgaagt tccttctggc ggccgagcaa atgaaaggtg ttgagtaatc
     4921 ctggcagacc cagataatgg tcgccatgca ggtgcgaaat caaaatgagt cctatgcggt
     4981 tgaaattaat gccaaagcgc cgcaagttga tttgcgttcc ctcgccacag tcgattaaaa
     5041 caaagcgctc atgcacattg agtacctgag cgcttgtgaa tctacccggc aaaggagctc
     5101 cggatccgga tcctatgacg gtgagattaa atgtcattta tctgctgata ataagtttgc
     5161 gggttactgt ctgagtgcct ttggcaacgg tgtagaaata cactccttca ggcagggcgg
     5221 ccacattcag tgcaatagtg ttttccccgg cattcactct gtgattgctt gctgaaacgg
     5281 tacgtccaac catgtcgctg atggtaacgg cgactattga agtttcgtta tttgttacaa
     5341 caatattggc aacatcggca gcaggattgg gatacatatc ggttacaccg aaagaataat
     5401 atggattttc tccaaaacca acgtttccta taacaagttt atagccgttt actgtatttg
     5461 gtaatgacat aggcattgac atggcttcac cataagctat aacccttatt ttcagtggat
     5521 aaacacctgt ttgtccggct gtggcagttc caataatagc aatgcagcca ttcgaaccgc
     5581 ctggaattga tactggagcg ccaacaccgg ttggagcacc cgaccaggtg aaaccgcttg
     5641 gaagaccaac tatttcaatt acatttatag agtcgatggt tgcagaaaag cccatatatg
     5701 aagtatcagc agggacaact gcagtaatta cgccggtata tgcaacacca atagttaccg
     5761 gtggcagatt gatcagggtg tccggaaaaa ttccaggggt agtgtactgt gcatcgggaa
     5821 cgcagggctg agcatttaat gcagctgcaa gaaataaacc tgcaaacagg gtaaaaattc
     5881 ttttcatagt atttttgttt tttgcaaata tagcaatgat tttacattat aacaatttat
     5941 gcaactattt ttttcagact tgatttgttg ataccctttt gaataatgaa caccgaacag
     6001 ggaagtgaga atgatgaagg agtatataca ttatactgat ttcttacttc atatttcctt
     6061 gcctgcctgt tgcagacagg ttcgatattc atgattcagt gttcgtgtct cggtgccttt
     6121 gtggcaaacc aaaaaaaagc ccgcattact gcgggctctt atattgacta gaatgaaaaa
     6181 actattcttc gtcagatccg tcggcttttt tgcctttggc tttcttttct ttcaggttag
     6241 caaagatatc gagatcgccc agagtttctt tctcagcatt agattttgct tttgagaaag
     6301 aatcgtcctg tctcttttca gaaggttcgc gtttcttgtc ttcttttact tcctggaaga
     6361 ttttgctatg cgaaacaacg attttgcgtg attctttcga aaattcaatc actttgaaat
     6421 caagagcttc gtccatttca gctttgcttc cgtcttcttt ctgcaagtgg cggttaggag
     6481 catatccttc aacaccataa ggcagagaaa ctacagcacc tttatcattt ttctcaatga
     6541 tggttccttt atgaatagaa tcaacagaga aaatggtttc aaacacatcc catggatttt
     6601 cttccagttg tttgtgaccg agactcaggc ggcggttttc gacgtcaaca tcaagtacca
     6661 caacttcgat ttcatcaccg attttggtga attcggccgg atgcttgatt ttctttgacc
     6721 atgaaaggtc gctgatgtga ataagtccgt caacaccttc ttcgagttca acaaaaacac
     6781 cgaaattggt gaaattacga accgtagctt tgtgacgtga attgatcggg taacgatctt
     6841 tgatattcac ccatggatcg gggataagtt gtttgatacc gagtgacatt ttgcgttcat
     6901 cgcggtcgag gctgaggata atggcttcaa cttcgtcacc gactttcagg aaatcctgag
     6961 ctgaacgcag atgctgcgac catgacattt cggaaacgtg gatgagacct tcaacaccgg
     7021 tagcaatttc tacaaatgcg ccatagtcgg ccagaacaac cacgcggcct ttcactttgt
     7081 caccaacttt gagttcggtg tcgagtgaat cccacggatg agcagtcagc tgtttcagac
     7141 caagagcgat gcgttttttg ctttcgtcga aatcgagaat aaccacatta agtttctggt
     7201 caagctcaac gatttcttca ggacgattga tgcgacccca gctgaggtcg gtaatgtgaa
     7261 taagaccgtc aacaccaccc aggtcgatga atacaccata ggaagtgatg tttttaacag
     7321 taccttcgag cacctgacct ttttcgagct tggcaataat ttcggccttc tgagcttcga
     7381 gttcgtcttc gataagcgct ttgtgagaaa caacaacgtt tttgaattca ttattgactt
     7441 tcacaatttt gaaatccatg attttaccga caaaaacgtc gtaatcgcgg attggtttca
     7501 catcgatctg tgaacccggg agaaaagcct cgatgccaaa tacatcgaca atgagaccac
     7561 ctttggtacg gcatttaacc tcacctttga tgacttcacc ggtttcgtac agcacattga
     7621 tgcgatccca tgccttgagc aggcgggctt ttttgtgcga cagaataagc tggccagtgc
     7681 catcttcctg actttcaaca taaacttcaa taacatcacc gattttcaga tcggggttgt
     7741 aacgaagttc attggcggcg ataacaccgt cggatttgaa gccgatatta acaaagattt
     7801 cgcgatcggt tttggcaatg acggtaccat caatcacttc tttttctgta atttggctca
     7861 gggtttttac gtagagacct tccagttttt cacgctctgc agctgaataa atctggtctt
     7921 tctttccgat cgcatcccaa ttgaattcct caacttttgc ggtggttgca gccgtggttt
     7981 ccagagctgg tgcctgctcc tctaggttag tgttttctgt tttgttaagt tcgtcactca
     8041 ttaatttttc tcctttcctt gtctggttct cagacaatag ggtgtttttg taaatgggct
     8101 gcaaaagtag aaattattcc ttagaatacc aaataaatat tcagaaattc agctttcagt
     8161 tgtcagcttt cagcaggtcg ctttttaagc tgtgaccagt gagcggtgag ctggttattg
     8221 gttaacggtt aatggctaac ggttaatggt tatcggttaa tggttaacgg ttaatggtta
     8281 tcggttaacg ggaaatggtt tttggttaat ggaaaattta ttttattcaa cacgtaacct
     8341 tttaacttta agaactttaa gaacttttca ctcgcaaagc tttgtgcgct aggcatcagt
     8401 cgacaggcga taggcgtaag gctttgcgca ttgtgcgttg tgcattgcgc tttgagccac
     8461 aagccgcatg ccctatgctt tatgaatttc caggtacttt ttcataagtc cttccggatc
     8521 gctgacggtt ttcagaatcc attgagctat aagctcttgc tgttctttta tgccaataaa
     8581 acgatcgact ttctgcgcaa tggacttatc gatgactttt tgtaaaaaca aagccgcaga
     8641 aacggaaata ttaaagcttt cggtaaatcc atacatgggc agcgccacgg taaaatcggc
     8701 cagttctatt gctttttccg acaaaccttc cttttcggtc ccaaaaacca gagccagcgg
     8761 tttgctcaca tccatttcat ccacaggaac cggatttccg tgaggtgttg tggcggcaat
     8821 tctgtaaccc tgccggcgca gttcggtata gcatttttca attgaatttt cttcattgaa
     8881 atgattgata gtcaaccatt tttcggcacc caacgcaatc tcttcattag gttgaaattt
     8941 gttcacattt tcaatggcaa agacattctg aatgccaaaa caatcacagg tccggagcac
     9001 ggcactggcg ttgtgtccct gaaaaagatt ttcgagtgca accgcaaaat gctgggtgcg
     9061 tttcgaggca acttccttaa tcctttccag tcgcttttct gtaataaaat cagtcagaaa
     9121 ctctgtcagg ttgtcgatgt caaggtcttt aagattgggt tcgaatgctt tcatatgcgg
     9181 cggaaaattc tgaggttaag gttaaggttg aggttaaggt tgaggttaag gttgaggtta
     9241 aggttgaggt taaggttgag cagctttcag ctgtgagaat aagattgagt tggagttgtg
     9301 ctaatgtatt gatttgctgt tgatttgaaa atttgatgat ttgaaaattt gaaaagaaat
     9361 caattcagct tctgctcaga gcaacgacgc ttcggtcggc acatattgct gacagctgat
     9421 tgctgttagc tacttgtaac gtctgtaacg cgtaacgatt gtaacgtcta cttctgcgaa
     9481 gcaagtgcat gaatacaaag ctgatagagc aggcgacagc ccaccgtttc atcccattga
     9541 ttttcatgat caggagctac ttcaaccaaa tcgaatccca caatatgccg gccgcttttc
     9601 accagtttct ttacgacata aacggcctgt tcaaacgtaa gtccttcagg tacaggcgtt
     9661 ccggtatgag gacacaaaga tggtttgaaa gcgtcgatat cgaaagtaat ataaacctgc
     9721 tgtggcaacg ctgccacaat ttcatcggtg atggttttcc aggtttctcc ttcaaacatg
     9781 cgctctgtca ttatggtatt gtaaaaagtt tcaattttat cagaagaagc tgcaaaatga
     9841 gcttcatcat tacaaagatc gcgaacaccc acctgaacca atttgcccat ttcgggaaca
     9901 ttttccaaca cattccgcat cactgaagca tgtgaaaact gaaaatcaag ataatgctgc
     9961 cgcagatcgg catgggcatc aaactgcaga acacccagcc ccggaaattt tttcgctact
    10021 tcggcaatag cactgaaact tatgctgtgc tcgccaccaa gcaatgccgg gattttgcca
    10081 aaagccagac acttgttcac tttggccgat acataggaat tcatctggac acaggcctca
    10141 tttacctcat cctgagcgcg tttcaggcga tcggggagag gttcgtcgct gttttcaaga
    10201 tggtcgatta tggttttcga tttgcggcgt tcaaaagaat tcagatccat aatccagtct
    10261 tcgaaggggg catagaaaat gccttttttc cattcatcgg gcaattgagt atcaaataaa
    10321 tcaatctgat acgatgcctc aagaatcgct tcgggtgcgt aggcagtgcc ttcacggtat
    10381 gaagttgtaa catcccaggg tacaggaata atcagtagag ccgcattgtt ttcgtctttg
    10441 ggtaatccga aaatgccgtt gtttttactg aaatttttgt ccggatcgaa tggaaatagc
    10501 atatttgtat atattgtgct gcaaaaatac aatttaatgt gttaatgcga taatcacgtg
    10561 aaacgtgaca agtgtgttga tatgctgatg atacgatttg aaaatttgaa aatgatcgta
    10621 gtatcctttg ttacccttgt caccttgtaa cgtctgtaac gtctgtaacg attgtaacgt
    10681 ctgtaaccgt gaaagtaaaa tcgtacagca aaaaaaaatc gcaaaatcct gtcaatcgat
    10741 gtaaaaaaga aatatatttg taaaaaaaac tacgaattat gggacttctc ggaaaaatca
    10801 aaggcgaatt tatagatatc attcaatggc ttgatcctac caatgatact atcattcacc
    10861 gtttcgaacg ctacaataat cagattaaat acggtgcgaa actcaccgtg cgtgaatcgc
    10921 aaatggctgt cttcatcaac gaaggccaga ttgccgatgt gtttggacca ggtatgtaca
    10981 cgcttgaaac acagaatgtt cctattcttt ctacattgaa aggatggaaa tatggtttca
    11041 acagcccctt ccttgctgaa gtgtatttta tcaataccaa aaattttacc gaccgcaaat
    11101 ggggcaccaa aaatcccatt atgatgcgtg atgccgaatt cggcatggtt cgtctgcgtg
    11161 catttggcaa ttatcagttc agggtgggtg atccagccaa attcattaag gaaattgccg
    11221 gcacagacgg agcttttact acggatgaga ttgaagggca aatcaaagat gtgattatga
    11281 cccgctttac cgattacgtg gctgaagcca aaattcctgt gctcgatctg gcctcgaatt
    11341 ataatgaatt gtcgaaagga attcaggagc acattaaacc ggagctcgat gagtatggtc
    11401 tgaatctgac taaattttta atcgaaaata tttcgcttcc gcctgaagtt gaagccatga
    11461 tcgacaagcg ctcgagcatg ggcattgttg gtgatttgaa tcaatatacc aaatttcaga
    11521 cagccaatgc catcgaagag gcagctaaaa atccgggagg cgatgctggt gccggaatgg
    11581 gaatgggtat gggttttgcc atggccaatc agatgtcgca aacgctcaat cagccaaatc
    11641 aacaacagca acagcagcag caagctccgc cgccaccgct gattcagtat tttgttgctg
    11701 tcaacggtca gcagtcagga ccttttccaa tgccaacgct acagcaaatg gttcaacaag
    11761 gttcgctgac taaagaaact cttgtgtgga ccaatggtat ggccaattgg gaacccgctg
    11821 gtcaggtaca ggcgcttgcc ggaatatttc cggtgactcc gccacccatg ccgccacaga
    11881 tgtaacacaa catttttcaa cgtcctgctt cggcgggacg ttctttgttt tgatcttaat
    11941 ttaaaatcag aaaatcaata tgagtgatct ttctccggaa tttgaaggca aacatgccga
    12001 aacgctcgaa actaaatgca ccaattgtgg tgcggttctt aattttgctc cgggcaccaa
    12061 tcatttgaaa tgtcagtatt gtggtgctga aaatgaaatt tttcctgccg aagcacccat
    12121 cgaggagctt gattttgaac agtatcttca ggatggcgcc atgaatgctg atacggttca
    12181 ggttgttact gtcaaatgtt cgggttgtgg cgcttcaact acttttcagc caaatatagc
    12241 cagcgatacc tgtgcttttt gcggtatgcc gattgttgtt aatgagtctt cgacagctac
    12301 tgttatcaag ccaaaaagtc tgttgccttt taaaattact cagaaagaag cttttgagtg
    12361 ttttctcaaa tggttgcgaa aattgtggtt tgcgccttcg gcgctgaaaa agtttgtaaa
    12421 tcagcccgat aaactcaggg gaatgtatat tcctttctgg acttatgatt ctgataccac
    12481 aacgcgctat accggcgccc ggggaaccca ttattacgtt actgaaacat atacttcgaa
    12541 cggaaaaaca gaaacaagac aggttcaaaa aacccgctgg acgccggttt ccggaaccgt
    12601 tttcaaattt tttgatgata ttcttgtggt tgcttctact tcgctgccca tgaaatatgt
    12661 caataaactt gaaccatggg attttgagaa tctggctcca ttcgacgacc gctatctgag
    12721 cggattcaga accgagacct atcagattga tctgaaaaat ggttttgagc tggctaaaaa
    12781 atttatggaa cctggaatca gagcgttgat cagacaggac atcggcggag atgaacagca
    12841 gatttattca gtaaatacac agtacagcaa tatcaaattc aaacatttgc tactgcccat
    12901 ttggatcagc gcctatcgtt tcaaaaataa agtgtatcgc tttctggtca atggccgtac
    12961 aggcgaggtt cagggcgaac ggccctacag tttctggaag attttcttct tcacattggg
    13021 ttgcattgtc gttattgcag ggcttctgtg ggcttttggg gtgtttaaat aaatgcttta
    13081 aaccctcgaa ggcatacgcg tcaacttaaa gtaatttcaa ggtatatctg attga
//
LOCUS       DAOPKE010000078        13032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5021_length_13032_cov_12.394313, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000078 DAOPKE010000000
VERSION     DAOPKE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_13032_cov_12.394313"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1041)
                     /locus_tag="PKY63_09685"
     CDS             complement(154..1041)
                     /locus_tag="PKY63_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00925.1"
                     /translation="MKKILISLTMVFGIIVMVNGQELTSKKGFTILPETGDYVIGFNA
                     VPVVDFFLNAADIMTNAGTTAQHPGYVTGFNNVLVGKYFLEDTKAIRGKLAINTGSMT
                     TKTFFDDPFDITANPTDPTQWGELEDVNKIGGSTIILGGGLEFRRGHNRVQGFYGGEL
                     LLGFASNSIHNTWSVEMNNESMTNGYTNGDGSALNGRIIDQKSGMAITFGLRGFAGIE
                     YFFAPKISIAAEFGWGFGMTTNPRGSVTVETWDAAAVASEEETFEGTNSGSSMGFQVD
                     DGIGQTLMPSAALMMYFHF"
     gene            1298..3685
                     /locus_tag="PKY63_09690"
     CDS             1298..3685
                     /locus_tag="PKY63_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00926.1"
                     /translation="MKIFLFLLQIVLVHMALAQNGVVRGVVRDQKTNQPVPFANVVVQ
                     NSTIGSVTDFDGNFVISGLSNGFVRLQVSSVGYTTKISPEIMVTNSNETFIEIYIAEN
                     ITTLSGVTVTAATFERNIESPVALQTIGISEIEKNPGGNRDISKIVQSFPGVGQTPAQ
                     RNDLIVRGGGANENRFFIDGIEFPNLNHFATQGASGGPASIINADFIREAKFYTGSFQ
                     VNNGNAMSSVLDMNLITPSKDKAVHRFAVGASDLALSYNGPLSGNTGLLVSYRRSYLQ
                     FLFAAIGLPFLPTYNDYLIKTKTTLKNSDEITFFSLGAYDVSRLNLERNETEEQRYLL
                     SYLPETNQWNYTIGATYRHFKKNGYINYILSRNMFRNQSFKFQDNIEVDSLRIQDYSS
                     DEIENKFRFENVFFTKGWRWLTGASAEYDRYSNSTYQKFFAGGSPLTLNYNSEFDFFR
                     FAVFAQVSGKIMNNRLGVNIGLRTDANTYDDEMLNPLNQISPRLSLNYSLTERWFATV
                     SAARYSQLPAYTTLGYSDNGVLVNKKNNLSAIGVNHFIAGVEFIPNDDSRATFEAFFK
                     GYDNYPVSLRDSVSLANKGGDFGVVGDEPVVSSGKGRAYGFEFQYRQKVIDKLNMLFT
                     YTFVRSEFSGLDGSFIPSAWDARHIINVLGSRNFKRNWTLGIKWKFSGGAPYTPYDEY
                     TSSLVQAWEAQGRPYLDYDAYNTLRFKPFHQLDVRVDKEYFFNKWSLTLYMDIQNAYS
                     FNAQSQDIFIPVSDASGNYVITNPSDPAALQRYEMKRIENSSGTLLPTVGIIVEF"
     gene            3698..4435
                     /locus_tag="PKY63_09695"
     CDS             3698..4435
                     /locus_tag="PKY63_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00927.1"
                     /translation="MKTIIFISVLGLMAFTSCRNSNRHLPEPLSLITNVKGAGHTFEV
                     SFFSGKQFNHPVIAVWLETSDGSYIQTLYISKSVATGYFQHGDTKSGRWAPGPRRRPA
                     TVPYWSHKRNVQEADGLFVPTAATAIPDAYTGPTPLKDFVLKSKSDSSLHQLVRLVLE
                     INQAFDFNRSWTTTRFPNDQYYATSGQPSLVYATDVIDLNTPAEKYEMKLLGHGHYSG
                     ATGELFTDVSGHTTALEIVKQVVVKVK"
     gene            4897..6720
                     /locus_tag="PKY63_09700"
     CDS             4897..6720
                     /locus_tag="PKY63_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00928.1"
                     /translation="MRLFLYFFLMMLLPVLVTAQNHKHDTLKRDTLLTHYFSLSPDFY
                     LMHDSLEFSADVESFNGFSPLNSFACVASNGLLGSPYQTLWFPEHVHQLHIDYFDFFY
                     KPDAYLFTRSGIPVFTDTLPFSVASYSNGYKREQYFDFLHSQPLTRQWHLTLDYRLIG
                     SPGAYKNQKNSLSNFFAITNYHTKSERYHFTAGIIFNRIFQQENGGIKYQNEFVDTTV
                     YDRALTDVYLYSAQNQMRQNDYFITNEIRFGGKKDGRHRLYLQHTFNLRRERHMYYDS
                     FDPDSAYYSVWNTASTADSVNHYAFENSITMGNRYSKYLRWYADVFVSSDHVYNTGTD
                     TLLSHKVLTAGVSWNFQNGFGLLIHGSTDVFPVSGGDREIHSEFISNDSLKWRPHLRF
                     SYSMRSPRYFFFQYSGNHFSWQHDWQQSNTIMGMGGIAYKNIDLTAYFTRFSNYMYFN
                     GSAFNQGGTGNALGIKASADVKFGRFYFVGTAGLQDVIHANYLNLPPWFAKAELAMRN
                     SVFKKALTLKTGISAWINGLYYADAYNPALQIFYMQRQTKTGGFVYPTAFVRAQIKRA
                     VVFVEIINFTAGFTKVNYWQIPGYPLPDRGFRFGVTWSFLN"
     gene            6730..7824
                     /gene="ychF"
                     /locus_tag="PKY63_09705"
     CDS             6730..7824
                     /gene="ychF"
                     /locus_tag="PKY63_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPF00929.1"
                     /translation="MALKVGIVGLTNCGKTTIFNCISDAKAEVSTFAFSTTKSNLGTA
                     HVHDPRLYELDKLIKADKVVPATVELVDIPGLAKGAGQGEGIGNSFLNDVRNVDALIH
                     VVRCFDDPLLPHMDGSIDPVRDKENLDFELQVKDLEQIDRKLQKVEKAFKTGDKSVKP
                     AYDSLLKFKTQLENLGNIRSLDLNDDDEKLIRELYLLTAKPVMYVCNVDDASAATGND
                     YSVKFTEAVKGENTEVIIVAGRAEAEIAELDETDRVDFLKDLNLAEPGVRRITRAAYS
                     LLNLISFFTVGGVENRAWSVPRNTPAPQAAGAIHSDLERGFIRAEVIAYNDMIQFKTE
                     AACKEAGKMRVEGKTYVVQDGDILHIRFNV"
     gene            7987..8334
                     /locus_tag="PKY63_09710"
     CDS             7987..8334
                     /locus_tag="PKY63_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00930.1"
                     /translation="MKILRLLLPVLFLGYMSSATLFMHTHYINGVAVVHSHPYKSADD
                     GSSGHQHSNQGFLLIAVLSHISVLVAAAGIVCLAAFVFYKTRQFVTRRKALIGLIHCS
                     AFGLRAPPFSVSC"
     gene            8414..10705
                     /locus_tag="PKY63_09715"
     CDS             8414..10705
                     /locus_tag="PKY63_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF00931.1"
                     /translation="MKPLFFVFLFLFLFESVSFAQHGVTDANVVGHVLNKKTGEHMPF
                     VSIAVKGTTIGCLTDYTGHYYCINLPVGHYTIVASMMGFKRVEQDVEVLADKVIELNF
                     ELEEDLVMLNTVVVSSNRDETNRQETGNIVNVLTPRSFENTNSTCLAQGLNFQPGLRV
                     ESNCQNCGFQQVRINGLEGPYSQILIDSRPIFSALAGVYGIEQIPANMIERVEIVRGG
                     GSALFGSNAIGGTINIITREPLGNSAVFSNSTSLIYGKTADINSSFNASVVSDDMKSG
                     LVIFGSARQRSPFDYDGDGFSEIGKIDSKNVGIRWYYKTSNYSKLSLEYHNLDEFRRG
                     GNKFDLLPHESDITEQAQHRINSGGLRYDLFSKNSKRKWSIYSSAQHIKRDSYYGAQQ
                     DMNAYGSTTDNTMVGGTQFSQSFDTLLFMPAKLTMGGEFSYNALNDIMLGYNRVLKQD
                     VSVASAFFQNEWKNKKYTIIGGARFDKHNLIEKPIISPRVNVRFTPSEMFAFRVGYST
                     GFRAPQAFDEDLHITAVGGDVVLIVLDPDLQTERSQSYSASIDFYKTIGKIQLNILAE
                     GFYTHLDDVFYLDEIGTDALGNIVLERRNGPGAVVKGVNIEGKLAPAAKWQFQFGFTF
                     QNSLYDETLSWSDDSSIVPQRRMFRAPDMYGYITSTYSPVDAFKLSISGTYTGPMLVQ
                     HFAGYIENDTEFLTPGFFDAMAKISYTFKLGKTNSLEINGGVQNILNHYQNDFDQGTF
                     RDAGYMYGPSAPRTVFAGLKFSL"
     gene            10907..11137
                     /locus_tag="PKY63_09720"
     CDS             10907..11137
                     /locus_tag="PKY63_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00932.1"
                     /translation="MKGIRTKIVIGKSPLFLILLIIGYAMGITHKVLYHYDIVTYLYA
                     FNMLLVATDLIIYFRYIGDNKRNLALKKADIE"
     gene            complement(11158..11751)
                     /locus_tag="PKY63_09725"
     CDS             complement(11158..11751)
                     /locus_tag="PKY63_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00933.1"
                     /translation="MKKRTAYIFFVLLVITGILHTWFFNRTRASHGYMDLFIGVYFVV
                     GFLTFFILSIVFYPKKKFVILSVSTFVLCISLNILLKQDFVKGKPILSVQLFHASDSI
                     PEIIQNTDLSFKLDFKKNGQCVLHKRFVDYSYSYPYDYYISNDTITIDEDVVTDSDSA
                     LTTRYVIDRAKKIIIPVEKDRNKLPCLNITYDKLEQY"
     gene            complement(11800..12273)
                     /locus_tag="PKY63_09730"
     CDS             complement(11800..12273)
                     /locus_tag="PKY63_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00934.1"
                     /translation="MMPSTGMKEINAGVAAGKSVESNNETYYSFHTGFGMWKYAPRRI
                     NYEAGFNLKLNNSAMNYVTLGPYINGSVRLGYLTDLFAETNYDFSLSNQNEKSFHLRA
                     GISYPKLFYSPVGVKFFGDFNYYSFDRSMTVFPSVGIYYSIPIYNERKKTESGSE"
     gene            complement(12301..12468)
                     /locus_tag="PKY63_09735"
     CDS             complement(12301..12468)
                     /locus_tag="PKY63_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00935.1"
                     /translation="MSKKNIVYAFLIFIGLAFAVVSIMVYFTKGRSKSWIAYKMKIGA
                     LLLTITAIGDG"
     gene            complement(12602..12832)
                     /locus_tag="PKY63_09740"
     CDS             complement(12602..12832)
                     /locus_tag="PKY63_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPF00936.1"
                     /translation="MRTKRAVERDLEIIGEAANRILKRDKEFSLEHVQKIIGTRNRIA
                     HGYDKISDELVWSIIINHLPKLKEEIVLKLQE"
BASE COUNT         3565 a         2577 c         2811 g         4079 t
ORIGIN      
        1 tttcgttacg tgttacaatc gtaacaaggt agcagggtga taacaattgt tatcaaatca
       61 gcataatttc agttcaacct tagtcttgac cttatcttta taaataaaaa aagccggcgt
      121 ttgccggctt ttttgtaaaa ggtcttctta gacttagaag tggaagtaca tcatcagtgc
      181 tgcagaaggc atcagggtct ggccaatgcc atcatcaacc tggaagccca tgctgctgcc
      241 actgttggta ccttcaaatg tttcttcttc tgaagcaaca gctgcagcat cccatgtttc
      301 aacggtaact gaaccgcgag ggttggttgt cataccaaag ccccagccga attcagctgc
      361 gattgaaatt ttaggagcaa agaaatattc gataccagcg aacccacgga gaccaaaagt
      421 aatggccatg ccacttttct gatcaataat gcggccgttg agggctgatc cgtcaccatt
      481 ggtgtaacca ttggtcattg attcgttgtt catttcaaca gaccatgtgt tgtgaataga
      541 gttgctggca aatccgagga ggagttcacc accatagaat ccctgaacgc ggttgtgacc
      601 acgacggaat tccaggccac cgccaagaat gatggtggaa ccaccaattt tgtttacatc
      661 ttccagttcg ccccattgtg tcgggtcggt aggattggct gtaatgtcaa acgggtcgtc
      721 aaagaaagtt ttggttgtca ttgaaccagt gttgatggcc agttttccgc ggattgcttt
      781 ggtgtcttcg aggaagtatt ttccaaccaa tacattgtta aaaccagtta catagccagg
      841 atgctgggct gtggtgccgg cattggtcat aatgtcagct gcattcagga aaaagtcaac
      901 aacgggaacg gcattgaaac caattacata gtcaccggtt tcgggaagaa tggtaaaacc
      961 tttcttgctt gtgagttcct gtccgttaac catgacgatg attccgaaaa ccattgtcag
     1021 agaaatgagg atttttttca taacagtcga ttttagttta gactttgttc gttattaatg
     1081 ctgcaaatgt acaatattat tcccatgttt tcatcgttaa gctgtgaaat atttatcaac
     1141 caacgattct tgatatctca tggctgggtt tgttttttgt aatgaattgc aaatcagcag
     1201 caatgttaat tcgacccgat tgtacgcatg atacatatca ctatgttgca aaaagaccta
     1261 cttttgtgat tatttttgca attaaataaa aatatccatg aaaatatttc tctttttatt
     1321 acagatagta ttggttcaca tggccttggc tcagaatggc gttgtcaggg gtgttgtacg
     1381 tgaccaaaaa acaaatcagc ccgttccctt tgctaatgtt gtggtacaaa attcaaccat
     1441 tggttctgta accgatttcg atgggaattt tgtgatttcg ggattgtcca atgggtttgt
     1501 cagactgcag gtttcatctg ttggttacac aaccaaaatt tcaccggaaa ttatggtgac
     1561 aaattcaaat gagactttta ttgaaatata cattgcggaa aatataacaa cactgagcgg
     1621 tgtgacagta acggctgcca cctttgaacg taacattgaa agcccggtgg cactgcaaac
     1681 gattggtatt tcagaaattg agaaaaatcc cggtggaaat cgcgatattt caaaaattgt
     1741 gcagtctttt cctggtgtag gtcagactcc tgcacagcgt aacgacctga ttgtgagagg
     1801 tggtggcgcc aacgaaaacc gcttctttat tgacggtatt gaatttccga acctgaatca
     1861 ttttgccacc cagggcgctt caggtggtcc tgccagtatt atcaacgctg attttattcg
     1921 ggaagctaaa ttctatacag gttcttttca ggtcaataat ggcaatgcca tgagctctgt
     1981 tcttgatatg aaccttatta ctccgagtaa ggataaagct gttcatcgat ttgctgttgg
     2041 agcctcggat ctggctcttt catacaatgg gccgctgtca ggaaacaccg gactgctagt
     2101 gagttatcgt cgctcttacc tgcagttttt gtttgctgct attggattgc cttttttgcc
     2161 aacctataat gattatttga ttaaaacaaa aaccaccctt aaaaacagcg acgaaattac
     2221 atttttcagt ctgggtgcct atgatgtcag ccgcttgaat cttgaacgta atgaaaccga
     2281 agaacaaaga taccttttat cctatctacc cgaaaccaac cagtggaatt atactattgg
     2341 tgccacttac cgacatttca aaaaaaatgg ctacatcaac tatattttat ccagaaatat
     2401 gttccgtaat caatctttta aattccagga caatattgaa gttgattcgc tcagaattca
     2461 ggattattct tcggatgaaa ttgagaataa attccgtttc gaaaatgttt tcttcaccaa
     2521 ggggtggcgt tggcttactg gtgcatcagc tgaatacgac cgctattcca attcaaccta
     2581 tcagaaattt tttgctggcg gcagtcctct gactttgaat tataattcgg aatttgattt
     2641 tttcagattt gccgtatttg cccaggtgag cggtaaaatc atgaacaatc gtttgggtgt
     2701 caacattggt ttaagaacag atgcaaacac ttacgacgat gaaatgctga atcctttgaa
     2761 tcagatttca ccccgtctga gtctcaatta cagtttgact gaacgatggt ttgcaacagt
     2821 atctgccgca agatattcgc agttgccggc atacactact ttgggttata gtgataatgg
     2881 ggtgttggta aacaaaaaaa ataacctttc ggccattgga gtgaatcatt tcattgccgg
     2941 agttgaattt attccgaacg atgacagcag agccaccttt gaagcatttt ttaaagggta
     3001 cgacaattat cctgtttcgc ttcgcgactc ggtgagtctt gccaataagg gcggcgattt
     3061 cggtgttgtc ggtgatgagc cagttgtttc gtccggaaag ggcagagctt atggttttga
     3121 gtttcaatat cgtcaaaaag taattgataa actcaatatg ttgtttacat acactttcgt
     3181 acgcagcgaa ttttcgggtt tggatggcag ttttattcct tcggcctggg atgcgcgtca
     3241 cattatcaat gtgcttggca gcagaaattt caaacgaaac tggacattgg ggattaaatg
     3301 gaaattttct ggtggtgctc catatactcc atatgatgaa tacacctcgt cgctcgtgca
     3361 ggcctgggaa gcacaaggga ggccatatct ggattacgac gcctacaata cgcttcgatt
     3421 caagccattt catcaacttg atgtgagggt tgacaaggag tattttttta ataaatggag
     3481 tttgacattg tatatggata ttcaaaacgc ttatagcttt aatgcgcaat cgcaggatat
     3541 ttttattcct gtaagcgatg cttcgggaaa ttatgtaatc acaaatcctt ccgatccggc
     3601 tgcattgcaa cgctatgaaa tgaagagaat cgaaaattca tcaggcacgc tgttgccaac
     3661 tgttggaatc attgtcgaat tttaaaaagg atctttcatg aaaacaatca tatttatttc
     3721 agtacttgga ttgatggctt ttacttcatg ccgtaattcc aacagacatt tgcctgagcc
     3781 actttccttg attacaaatg tgaagggtgc cggtcatact tttgaagttt cgtttttttc
     3841 aggcaaacaa ttcaatcatc cggtgattgc tgtctggctt gaaaccagcg acggtagcta
     3901 tattcaaacg ctgtatataa gtaaatctgt agcaaccggt tactttcagc atggtgacac
     3961 caaaagcggc agatgggctc cgggtccgcg tcgcaggcct gctactgttc cttattggtc
     4021 gcataaacgt aatgtgcagg aagctgatgg actatttgta ccaaccgctg ctacggccat
     4081 tcctgatgcc tacacaggac caactccgct gaaagatttt gttctgaaat caaaaagcga
     4141 ttcgtcattg catcagttag tgcggctagt gcttgaaatc aatcaggcat tcgactttaa
     4201 ccgaagttgg actacaactc gttttccgaa cgatcaatac tatgcaactt caggacagcc
     4261 atcgcttgtt tatgcaactg atgttattga tctgaacacg ccggcagaaa aatatgaaat
     4321 gaaacttctt ggccatggac attattccgg cgctacgggc gaacttttta ccgatgttag
     4381 cggccacact acagcgctgg agattgtgaa acaggtggtc gtaaaggtaa agtaaacctc
     4441 ttctacagtg gtgaccagtg agttgtgatc ggtgagctgg gaaatggtta atggttatcg
     4501 gcaattcata ctacacttct tccttcgtcc gccgtggcgg atgtgcagat gctataaacg
     4561 tttaccccaa aaattaagat agttggttct tttgaagacg actactccgc ctccggcgga
     4621 gtaaactccc tcgtcgaaca ataatagccc gaagaattca cgttttacgt gatgccctga
     4681 atgctgagta tgcgaagctc agggcttcgg ggacagagca cgcgaagcac ctttgagatg
     4741 gaaggttacg cgtcttagac gcgcaagacg cgccgaataa atgataaaca ttaaattcga
     4801 tgttcataat tcaatcacac ttccgatcta taatcaaaaa tctcaaatcc ttgttcttaa
     4861 atcatttctt cctaactttg catcttaaac tgaatcatgc gcttattcct gtattttttt
     4921 ctcatgatgc ttctgcctgt gttagttaca gctcagaatc ataagcatga tacgttgaag
     4981 cgtgatacat tgctgacgca ttatttttct ttgtcgcccg atttttatct catgcatgat
     5041 tcgcttgagt ttagcgcgga tgttgaatct ttcaatggtt tcagtccttt aaacagtttt
     5101 gcctgtgtgg cgagcaatgg tttgctggga tcgccttatc agactctttg gttcccggag
     5161 catgttcatc aattgcatat agattatttc gattttttct ataagcccga tgcttatttg
     5221 tttaccagat ccggtatccc ggtttttacc gatacacttc ctttttcagt tgcatcttat
     5281 tccaacgggt ataaaaggga gcagtatttt gattttttac attcccaacc gttgaccaga
     5341 caatggcatc ttacgctcga ctataggttg atcggttctc ccggtgctta taaaaatcag
     5401 aaaaacagtt tgagtaattt ttttgcaata acaaattatc acacaaaatc agagaggtat
     5461 cattttactg ccggcatcat tttcaacagg atttttcagc aggaaaacgg cgggataaaa
     5521 taccaaaacg agtttgttga tacaacagtt tatgatcgtg cgctcaccga tgtgtatttg
     5581 tattcggcgc aaaatcaaat gcggcagaat gattatttta taactaatga aattcgcttc
     5641 ggcgggaaaa aggatggaag acatcggttg tatttgcaac atacattcaa tctgcgtcgt
     5701 gaacgtcata tgtattacga ctcctttgac ccggacagtg catattattc tgtttggaat
     5761 accgcttcaa cagccgatag tgtcaatcat tatgctttcg aaaattcgat aacaatgggc
     5821 aatcggtatt caaaatactt acgctggtat gccgatgtat ttgtatcatc cgatcatgtt
     5881 tacaataccg gaactgatac attgctgtcg cataaagttc ttacagcagg tgtttcatgg
     5941 aattttcaaa atggtttcgg gctgttgatt catggttcaa cagatgtttt tcctgtttcg
     6001 ggtggtgatc gcgaaataca ttctgaattt atcagtaatg attcgctgaa atggcgcccg
     6061 catttgcgat tcagctatag catgcgtagc ccccgttatt ttttctttca gtactctggc
     6121 aatcatttca gctggcagca cgactggcag cagtcgaaca ctattatggg aatgggcggt
     6181 attgcttaca aaaatatcga cctgactgct tattttacaa ggttttcaaa ttacatgtat
     6241 ttcaacggtt ccgcatttaa tcagggaggg acaggcaacg ctctgggaat aaaagcttcg
     6301 gccgatgtga aatttgggcg tttttatttc gttgggacag ccggcctgca ggatgttatt
     6361 catgctaatt atctgaattt gccgccttgg tttgccaaag cggaactggc tatgcgaaac
     6421 agcgttttca agaaagcact gactttgaaa accggtatca gtgcatggat taatggcctc
     6481 tattatgccg atgcatacaa cccggctttg cagatttttt atatgcagcg gcaaacaaaa
     6541 acaggtggat ttgtttatcc aactgccttt gtccgtgctc agatcaagcg cgctgttgta
     6601 tttgttgaga ttattaattt tactgccggt tttacaaaag taaattactg gcagattccc
     6661 ggatatccgc tccccgacag gggtttccgg tttggtgtaa cgtggagttt tctaaattaa
     6721 aagattgata tggcattgaa agttggcatt gtaggcctta cgaattgtgg taaaacaact
     6781 atttttaatt gtatctcgga tgcaaaagct gaggtgagta cctttgcatt cagcaccacc
     6841 aagagcaacc tgggaacagc tcatgtccac gatccccgtt tgtatgaact tgacaaactt
     6901 atcaaagctg ataaggttgt gccggcaacc gtcgagctgg tcgatattcc gggattggct
     6961 aagggtgcgg gacagggcga aggtatcgga aattcttttc tcaacgatgt gcgcaatgtc
     7021 gatgcactga ttcatgtggt tcgctgtttc gatgacccat tgttgcctca catggatgga
     7081 agtattgacc ctgtgcggga taaggaaaat cttgatttcg aattgcaggt gaaagacctt
     7141 gaacagattg accgtaaact gcagaaagta gaaaaagctt ttaaaaccgg cgacaaatca
     7201 gtaaaaccgg cgtacgacag tctgttgaaa tttaaaacgc agctcgaaaa tctgggcaat
     7261 atccgttcac ttgatttgaa cgatgatgat gaaaaactca ttcgtgaatt gtatttactg
     7321 actgcaaaac cggtgatgta tgtttgcaat gtggatgatg cttccgctgc aacaggcaat
     7381 gattattccg taaaatttac tgaagcagtt aaaggtgaaa acaccgaagt catcattgtt
     7441 gcaggccgtg ccgaagctga gattgctgag cttgacgaaa ccgatcgcgt tgattttctg
     7501 aaagatttga atcttgccga acctggtgtt cgccgaatta cgcgtgctgc ttactcatta
     7561 ttgaatctga tttcattttt caccgttggt ggtgtcgaaa accgcgcatg gagtgttccg
     7621 cgcaatacac ctgcaccaca agccgcaggc gccattcaca gcgatctcga acgcggcttt
     7681 atccgtgctg aagtgattgc ttacaacgat atgattcagt ttaaaaccga agctgcctgt
     7741 aaagaagctg gcaaaatgcg tgtcgaaggt aaaacctatg ttgttcagga tggagatatt
     7801 ctgcatattc gttttaatgt ttaattaaat ttgaaaaagt agataaatgg gtaatatttg
     7861 tagattgatc aataaatatt gctgcttatt cggtgttggg tgtgataaag tgttatttgt
     7921 attgattata aattaaaata tttttctact tttgtaatgt aaacctgaag aatgaaaaag
     7981 gtgtgtgtga aaatattgcg tttgttgctg ccggtcctgt ttttgggcta tatgagcagt
     8041 gcaacgttgt ttatgcatac gcactatatc aatggagttg ccgttgttca ctctcatcca
     8101 tacaaatcgg ccgatgacgg ttcttcgggt catcaacata gtaatcaggg gtttttgctg
     8161 attgctgttc tttctcatat ttctgttttg gttgctgctg ctggaattgt ttgtctggcg
     8221 gcttttgttt tttataaaac cagacagttt gttaccagaa ggaaagcttt aatcggattg
     8281 attcattgct ctgcttttgg tttgagggct ccgccctttt cggtttcatg ctaaatactg
     8341 tttcggatta atctccggac cccgcttctt atttaagttg cggtatgttt caactgttta
     8401 attgaagtat tttatgaaac cacttttttt tgttttttta tttctttttt tgtttgaatc
     8461 tgtgtctttt gcacagcatg gtgttacgga tgccaacgta gtggggcatg tgctgaataa
     8521 aaaaaccggt gaacacatgc cttttgtaag cattgctgtt aaaggaacca ccattggttg
     8581 tctgaccgat tataccggac attactattg tattaatctt cctgttgggc attataccat
     8641 cgttgcatca atgatgggat tcaaacgtgt tgagcaagat gtggaagttc ttgctgataa
     8701 agtgattgaa ctgaattttg aactcgaaga agatctggtg atgcttaaca cggtggttgt
     8761 gtcttcaaac cgcgatgaaa ccaatcgtca ggaaaccgga aatatagtga atgtcctcac
     8821 tccgcgttct ttcgaaaaca ccaacagcac atgtctggcg cagggattga attttcagcc
     8881 cggtttacgt gtggaaagca attgtcagaa ttgcggattt caacaggtgc gcatcaatgg
     8941 acttgaaggt ccttattctc agatattaat tgatagcagg cccattttca gtgcactggc
     9001 cggcgtttat ggaattgaac agattcccgc caatatgatt gaacgggtcg aaattgtgcg
     9061 tggtggcgga tctgcattgt ttggttcaaa tgccattggc ggcaccatca atattattac
     9121 acgtgaaccg cttggaaatt ctgctgtttt cagcaacagc acaagtttga tttatggaaa
     9181 gactgctgat attaattcat cgttcaatgc ttcggtggtg tccgacgata tgaagtccgg
     9241 attggtgatt tttggttctg ccagacagcg ttcgcctttt gattatgatg gtgatggttt
     9301 tagcgaaata ggtaaaattg attcaaaaaa tgttggaatc cgctggtatt ataaaaccag
     9361 caattacagc aaactctcgc tcgaatacca caatttggat gagtttcgtc ggggcggaaa
     9421 taaatttgat ttgctgcctc acgaatcgga tattaccgaa caggctcaac accgcattaa
     9481 ttcaggcggt ttgcgctatg atctgttttc aaaaaacagc aaacgcaagt ggagcattta
     9541 ttcatcggct cagcatatca agcgtgatag ttattacggt gctcagcagg atatgaatgc
     9601 atatgggtca acgaccgaca atacaatggt tgggggaaca cagttttcgc agagtttcga
     9661 tactttgctt tttatgccgg ctaagttgac aatgggtggt gaattttctt acaacgcttt
     9721 gaacgatatt atgcttgggt acaatcgggt tctgaagcag gatgtgtcgg ttgccagcgc
     9781 ttttttccaa aatgaatgga agaataaaaa atataccatt attggtggag cgcgtttcga
     9841 taaacacaac ctgattgaaa aacccatcat cagcccgcgt gtgaatgtgc gttttacacc
     9901 ttctgaaatg tttgcttttc gtgtaggtta ttcaaccgga ttccgcgcgc cgcaggcgtt
     9961 tgacgaagac ctgcatatta cggctgtggg aggcgatgtt gttttgattg ttcttgatcc
    10021 tgatctgcag accgagcgct cacaaagcta ttcagcatct atcgattttt ataaaacgat
    10081 tggtaaaatt cagctgaata ttcttgccga aggtttctac acacatcttg atgatgtatt
    10141 ctatttggat gaaatcggaa ccgatgcatt gggaaacatt gtgctggagc gccgtaacgg
    10201 ccccggcgcg gttgtcaaag gggtgaatat tgaaggaaaa ttggctcctg ccgccaaatg
    10261 gcaatttcag tttggcttca cctttcaaaa cagcttgtat gatgaaaccc tctcctggag
    10321 cgatgattca tcgattgtgc cgcaacgcag aatgttccgt gctccggata tgtatggcta
    10381 tattacttct acctattcgc cggtcgatgc tttcaaattg tcaatatccg gaacatatac
    10441 cggccctatg ctggtgcagc atttcgcggg ttacattgaa aatgataccg aatttttaac
    10501 ccccggtttt tttgatgcaa tggcaaaaat ttcttacact tttaagcttg ggaaaaccaa
    10561 ttctttggag ataaacggag gtgtacagaa tattctcaat cattatcaaa acgattttga
    10621 ccaaggtact tttcgcgatg ctggctatat gtacggccct tctgctccgc gtactgtgtt
    10681 cgccggcttg aagttttcgc tgtagctttt ttcaaatttg tggtttgttt ttcaagaccg
    10741 ttgtctggtt ttccaggtga tggtcttgtt ttttttcaga atgttttttc gaaatagttc
    10801 attaattttg cattctcata tctatttaat tatgcaagct cactcattca gtatattcga
    10861 agttttcatg ctcctttgct ttgcatgtag ctggcctatt tctattatga aaggcattcg
    10921 cacaaagata gtgattggca aaagtccgtt gtttcttatt ttgctgatta ttggttatgc
    10981 catgggcatc acccacaaag tgctctacca ctacgatatc gttacgtatt tatatgcatt
    11041 caacatgctg ctggttgcca ccgacctgat catctacttt cgctacatcg gcgacaacaa
    11101 gcgtaatctg gctttgaaga aggcggatat tgagtgagca aaatgaattt gttgaagcta
    11161 atactgctct aatttgtcgt aggtaatatt caaacatggc agtttgttcc tgtctttttc
    11221 aaccggtatg attatttttt ttgccctatc tataacatac cttgtggtca atgcactgtc
    11281 gctgtcagtc actacatctt catctattgt tatggtgtcg tttgaaatgt aatagtcgta
    11341 tggatatgaa taagaatagt ccacaaatcg tttatgcaaa acgcattgtc cgttcttttt
    11401 aaaatctaat ttaaaagaca agtctgtatt ttgtattatc tcaggaattg aatcgcttgc
    11461 atgaaaaagc tggactgaca gaatgggttt gccttttaca aaatcctgtt tcaaaagaat
    11521 gttaagcgaa atacacagaa caaatgtcga aacggaaagt atgacgaatt tctttttagg
    11581 ataaaatacg atcgaaagta taaaaaaagt taagaatccg acaacaaaat atactccaat
    11641 aaacagatcc atatagccat gtgatgctct agtcctattg aaaaaccatg tgtgtaaaat
    11701 ccctgtaatg acaagaagta caaagaaaat ataggcagtt cgttttttca tatattgaga
    11761 cattagttaa aattgaagaa taaattctct tgtaatccat cactcactcc cgctctccgt
    11821 ttttttccgc tcgttataaa tcggaataga ataataaatt ccaaccgacg gaaacacagt
    11881 catgctgcgg tcaaacgaat agtagttgaa atcgccgaag aattttacgc caaccgggct
    11941 gtagaaaagt ttcggatacg aaatgccagc cctcagatga aagcttttct cgttctgatt
    12001 gctgagcgaa aaatcataat ttgtttctgc aaaaagatcg gtcagatagc caaggcgcac
    12061 cgaaccattg atgtagggtc ccagtgttac ataattcatg gccgaattgt tcagcttcag
    12121 gttgaatccg gcttcataat taatccttct gggtgcgtat ttccacatcc cgaaacctgt
    12181 gtgaaacgaa taataagttt cattattgct ttcaacagat ttgccggcgg ccacacccgc
    12241 attgatttct ttcattccgg tgctgggcat cattctataa caagtcgcct gtcgcaccct
    12301 tcatccatcg ccgatggccg taattgtcag caacagcgcg cctattttca ttttatacgc
    12361 tatccacgat ttcgatctgc ccttcgtaaa atacaccatg atggaaacca ccgcaaaggc
    12421 aagcccgata aaaatcagaa aagcgtacac aatgtttttc ttactcatgg aatatctgat
    12481 ttaatgcggc ttttagcttt gaataatggt tgttttttta cgcagaatgc aaaaatacac
    12541 aactacattt tgcagttctg tagaacaaat ctatgaaatg ttttgtaaaa atgaagatgt
    12601 tttactcctg gagtttcaaa acaatctctt cttttaattt gggaagatga ttgataatga
    12661 tgctccagac aagctcatcg gatattttat catagccgtg ggctattcgg tttcgggttc
    12721 cgataatttt ctgtacatgt tccagagaaa attctttgtc ccgtttcaga attctgttag
    12781 cggcttcacc aattatttca agatctcgtt caacagcacg tttggtgcgg atatcacgga
    12841 catactctta aaatattttt gagcgatcag caaagtaact ttccacctct tgaatggcct
    12901 gaagaatatc gtaaagccat gttttgattt caacatccat aaatcagctg ttttgatgaa
    12961 tcaatggatt gttttaaatc aggcgtgtcc ggtttaggta attcacaaat cacgacaaac
    13021 agcttgcaga gc
//
LOCUS       DAOPKE010000079        12866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5130_length_12866_cov_9.493638, whole genome shotgun sequence.
ACCESSION   DAOPKE010000079 DAOPKE010000000
VERSION     DAOPKE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_12866_cov_9.493638"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKY63_09745"
     CDS             <1..464
                     /locus_tag="PKY63_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00937.1"
                     /translation="DSVFSFISASIGVRPLSEPCADFTGFPNAKMSPPATIPGWENAR
                     PEQSSHLHKKFLEKSRISNIPKMPAQSTVSLYMVFGSENMRSAEIIYSKRSAEVLSGC
                     LFEERSDELYPVGADRLFSRKPYQATSFSGVYAAESGNTFLEVYPELVEGQ"
     gene            complement(939..2519)
                     /locus_tag="PKY63_09750"
     CDS             complement(939..2519)
                     /locus_tag="PKY63_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF00938.1"
                     /translation="MKRLFTVLVLSFVAFASIAQWTLQNTAFTSTSRGIKFISVVDSN
                     TVWATAYDGANTANNIQEFTKTTNGGTSWTPYVVGGYTGYGLSMICAVDNNNAWIPAY
                     NASTGGGVILHTSDGGTTWNPQTTASFAAPNGFPNVVHFWNADTGFCMGDPNEGYFEL
                     YTTEDGGTTWTRVPSGNIPAPVSGEYGVTGYYSVVGSTIWFSTNKSHILKSSDYGHTW
                     SLITVPIATDNQFAVKFRSLNDGIIRMNTTPYSAYITHDGGATWNIISFYGNWYNNDY
                     CHVPGTASTWVSVGADATTPFMGISYSTDDGANWTDFTSMDTTQHLAVAFADHKTGWS
                     GSFHNYATDGIFKHYGSTFVLDTCSTFYSAFTLSEDTVFLPSTAEVIFTDASSPAATD
                     WAWDFGDGASASTQNPSHTYTSTGTYTVQLIAHHGVCVDTSTATVVVLVGSAIDEQNY
                     SLTLYPNPAADAVYINGLPSGEYLAEWISTDGRVIESVRCNETTILNTENIENGLYIL
                     RFSNESINIRKPLVIRHN"
     gene            complement(2740..3708)
                     /gene="dusB"
                     /locus_tag="PKY63_09755"
     CDS             complement(2740..3708)
                     /gene="dusB"
                     /locus_tag="PKY63_09755"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPF00939.1"
                     /translation="MNLPGLENIQTPLILAPMEGITDTVFRRICLSEGADAGISEFIS
                     SEAIIRNIEKSLRKMDAAHEEELRWVQIFGNKPESVAEAARVAEENGAHIIDLNFGCP
                     VKKVVSKGNGAALLRNLPLMQEIAAATVNAVQIPVTAKTRLGWDAQNIVVQEAALRLQ
                     DAGVKALAIHGRTRAQQYGGTADWNPIAAVKAVLQIPVIVNGDINSPEKALEAIKITG
                     SDGLMIGRAAIGNPWIFGQIRALLQGGTIPVVTIAHKTATCRRHLLESINLKGERRAV
                     IEMRKQYSQYFKGLSDFKPFKAELMAALSADEVNRIIIKIAEHYSF"
     gene            4126..5058
                     /locus_tag="PKY63_09760"
     CDS             4126..5058
                     /locus_tag="PKY63_09760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPF00940.1"
                     /translation="MQSPLKGKHPAIQLISFVGLSLAGVIAVMFIGMLAGVFIWGPAF
                     TDSFLVAGKGEDYILHMKYLQILSHLGMFIVPAFVFGWLAGGNAIRYFSLNRGMSVPV
                     FLVSIALIFLSQPLINLLAEWNSTLSLPESLSGIESWMKESEEQATALTGLFMKDTLW
                     SSMVFNLLMMAVIPALGEELVFRGVLQRLLIKWFRPAWISILFTAIIFSAFHMQFYGF
                     LPRLILGLILGYAFYATGRLWVPIVIHFINNGMVVVVTWLYELGFNQMNPDTFGNFND
                     NPLFLIISLFLFTASLVIFAKLAKKHRYEQSAAI"
     gene            5036..5497
                     /locus_tag="PKY63_09765"
     CDS             5036..5497
                     /locus_tag="PKY63_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPF00941.1"
                     /translation="MNKAQLYNRIFVQLEDLLKKSPAPVASMATVSAVLFHKLKHYSW
                     CGFYYLNSGDLIVGPYQGPVACQMLARHKGVCWAAIDTRKSQLVPDVEQFAGHIACDS
                     RTKSEVVVPLRNAYGEVVAVFDVDSHVLNSFDADDVQGIEKIMQLLEPFEI"
     gene            5494..6045
                     /locus_tag="PKY63_09770"
     CDS             5494..6045
                     /locus_tag="PKY63_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00942.1"
                     /translation="MITLVLALLAIGFLQISVTYRHALLFSKASIGKLLLVSFLLTIM
                     NNLMAWVALLFTNAIRDTMFFDSTYAAMFILAILAFKAYFNTRRSKIADNVFDITQPK
                     LLVLLGLATSFEVFIAVMGAGMIETELVPALIIVLAVSLFFSMLGFLAGRRPSQITSI
                     RVFILIASVFYLLAALSTIYFLL"
     gene            6079..6519
                     /locus_tag="PKY63_09775"
     CDS             6079..6519
                     /locus_tag="PKY63_09775"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPF00943.1"
                     /translation="MKIRISKEFSFEMAHALDGYDGLCRNVHGHSYQLTVTLTGEPES
                     DIQSPKYGMVMDFGDLKKIVQQAIVLQFDHAFVVRKGTLSHLDKNEMFSRLIEVDFQP
                     TSENLLVHFASLINSMLPSRVSLYSLRLRETGTSYAEWFASDNL"
     gene            6523..8352
                     /locus_tag="PKY63_09780"
     CDS             6523..8352
                     /locus_tag="PKY63_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPF00944.1"
                     /translation="MKTAYYLRKMRMKLHENISGIKRPAVRWSFIGILAASMIGFFLV
                     LAEVGFDTDFVLREWYNEKIEWIINFLMVMYSIQVLMVLLPGPPGITPLNIIVKIILL
                     ALLSLHFFDFIPFDISLESTSGYKLEWLFNYGLVIIIMFRSWSRIVNNIYSSNITSEK
                     LFIVSFISLIAVGTILLLLPVSTTEQISFTDALFTSTSAVCVTGLTTLDTATDFTWFG
                     KTVILFLFQMGGIGVMTFTSFFASVGRSKHSLSEGNALTSMVVAKNMRKLFTALYAVI
                     IVTFIVESFGTVLIYLQLPPDEFPDNISRFTFALFHAVSSFCNAGFSLAPNGLMNDIF
                     YHEWGIQMTMAWLIIFGGLGFFIITNYMERFALGMRNFIKHTILGHASKFEPNLININ
                     SRLVVRTTFWLLLLGTGLFLFFENDALLSGLRWDEKLYTAFFSTVTARTAGFNTLDYE
                     SIMLPSTLLIIILMWIGASPGSTGGGIKTTTFAIAFLTAFSLGRGKTTTHFHNRELSR
                     QSGRKAQVIIFFSVFAILIATMFMAWFEPGVSMQRLLFEAFSAFGTVGLSMNLTMFLS
                     EESKFILIMLMFIGRIGIITLFVAFIRKTRFLLFSYPEEELFI"
     gene            8365..9045
                     /locus_tag="PKY63_09785"
     CDS             8365..9045
                     /locus_tag="PKY63_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPF00945.1"
                     /translation="MKIMVIGLGNFGTALAIKLTRLGNEVIGIDRSRHKTESIKEFVT
                     TVVTVDITEASAVAQLPVAEMDVIVISIGENLGDSVIATALLKDNGAKRICCRAISET
                     HFSILMNMGVHHIFTPEVDAASNSVSTVLYELVHTAYVIGDDYKVCEIYVPKRFVGLK
                     VKDLTLNSHGLKLLLFRQAKSELHPQYMVKMIFEDRVKDYDEIIMKKDDLLVLFGLKK
                     DLDRFVAL"
     gene            complement(9258..11327)
                     /locus_tag="PKY63_09790"
     CDS             complement(9258..11327)
                     /locus_tag="PKY63_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00946.1"
                     /translation="MNKRILLFALMLLTAGISMAQVGISSVSITPDASAMLEVRSTNS
                     GVLVPRVTLSSTLIAAPVTTPATSLLVYNTATAGDVTPGFYYWDSSKWVRLLSGAMPA
                     DAWKLVGNAGTVATSNFIGTTDGVDFVTRTNNTERARILSAGNVLVNRTTALYATDLF
                     EAQGNATFPDAISGYTNQAAGTGVYGGNTTASGAGSGCGVYGATFQTGASGVFGDGDA
                     YTTGTIGYTEAAGFDGVSGYNVATSGAGLGSGVYGESMQTGAAGVWGSGSTFTRGVLS
                     TNNSALYATTQTQNAAAGGDALYAVNTATAGTGTGTAIYASSNQYGCASIMSVLNSTS
                     YYANSALSAINGSTYETTTPKGIGAGVTADDGIGIMGVNSAATGTATGDGVYGTTAQG
                     AGLGVYGYNSHTNGTGVMGTGNNASGAYLTDGSGGAFTGTNSGSFSKATSTTGTAVLG
                     AGNNLSAYTLTGEGSGGAFTGATIGVYGTAHNSANDTWGGYFDNSYGAYAYVGGTTTG
                     GTDIKISGSGTAATIVSRPDNTKAIMFCTEAPEILFQDYGVGKLVNGRAHIELDPILV
                     QNILVDANHPLKVFIQLEGECNGVYVTNKTQTGFDVVELSNGQSNIEFSWSIVANRAD
                     AMRDNGTYSKNADVRFPDAPLLLKKEAATESTKNTIDLSTGTDDHSKQIITKGKKRNV
                     SAKKARQ"
     gene            11641..12777
                     /locus_tag="PKY63_09795"
     CDS             11641..12777
                     /locus_tag="PKY63_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00947.1"
                     /translation="MIRFIFLLMISVLFVACGHNNEKSVPVADDSLTVAQSKLVYDTS
                     LTSVAQLMAGIKPDDTSGKAAIVQKDVWENHADSISLLFENAEKKNLVFMRQWAAAEL
                     PEGKDTAATLLYAFSGPDYLYANTFFPCAKKYILFGLEPVGRIPVLDTSKNQTLFFNN
                     IRRALKTSLSRNFFITLHMSGDLRASEYNGVTSILMLYAAYTGHKIKDISYVTVDTNG
                     HVQPCSYDSLNVCHQTAGVCLNLIDSLGRYKELTYFSFNAENSAFEKSTVKKYLESLN
                     GKTYGMLKAASYLMHYDGFMKIREVFTAKVSAVLTDDTGLSYRTLKAVFPKLQLYGKF
                     SQPVDMFNYINLRDLQLAYDSLQAKPIRFTYGYGYGQKLMIGRK"
BASE COUNT         3274 a         2597 c         2832 g         4163 t
ORIGIN      
        1 tggattccgt gttttcattc ataagcgcct ccattggagt gagaccgttg agtgagccat
       61 gtgctgattt tacaggtttt cccaacgcca aaatgtcacc tccggcgacg attccgggat
      121 gggaaaacgc gcgtccagag cagagttcgc atttgcataa aaaatttctt gagaaaagcc
      181 gtatctcaaa tattccgaaa atgcccgctc aaagtacggt gtcgctatat atggttttcg
      241 gatcagaaaa tatgcggtca gcggaaataa tctattcgaa gcgcagcgca gaagtactat
      301 ccggatgtct gtttgaggag cgtagcgacg agttatatcc ggttggagct gaccgtttat
      361 tttcccgaaa accatatcag gcgactagct tttctggagt ttatgccgct gaaagcggta
      421 atacttttct tgaagtttat cctgagcttg tcgaaggaca atgaaaaaga aaagtacgct
      481 cggcagtatg ccaagattat atgctattgc gagattggtg tgataggtgc gtttcattga
      541 atgaaaatac aaacatagca tagcttgccg taaaatctct cacagaaagg ttatttattg
      601 cgtttccatc tgcttattca atgtgagaat aagtacgata tagaaatatt cagaggttcc
      661 cataaatcaa aaaaaatata gctttgccat ctgcgactga aataattact taagcttgat
      721 attgtattta ataaaaatgt tttccttacg tttccagtat tacgtcatta tttatcgtaa
      781 aaggcataat atcaaatata tacaaaatta tgcagcgact cctccggagt catccaaatt
      841 catggattat tcaatgttta cttttggaat aaaaaaaagc aggcgcggat acctttctgc
      901 gcctgctttt aggtaagctg tattttcagc ttgaattttt agttatgacg aatcaccagc
      961 ggcttcctga tatttatgct ctcattgctg aaacgtaata tgtagagccc gttttcaata
     1021 ttttccgtat tcaggatggt tgtttcatta caccgaaccg attcaatcac gcgcccatcg
     1081 gtcgaaatcc attcggcaag atattcaccc gatggcaatc catttatata aacagcatca
     1141 gctgccggat ttggataaag agtaagggaa taattctgtt catctattgc tgagccaacg
     1201 agtacgacta cggttgctgt gcttgtatca acacagactc catgatgagc aatcagttgt
     1261 acggtgtaag tacctgtcga agtataggta tgggatggat tttgtgtaga agccgatgct
     1321 ccgtcaccaa aatcccaggc ccaatcggtt gcagcaggcg aactggcatc ggtaaaaatt
     1381 acctcagcag tagatggcag gaaaacagtg tcttctgaaa gtgtgaaggc agaatagaat
     1441 gtagagcatg tatcaagaac gaatgtgctt ccgtaatgtt tgaaaatacc atctgttgca
     1501 tagttatgaa aagatcctga ccatccggtt ttatggtctg caaaagcaac agccagatgc
     1561 tgtgtggtat ccatactggt gaaatctgtc cagtttgccc catcgtcggt tgaataggaa
     1621 attcccatga aaggcgttgt ggcatcggcg ccaacactaa cccatgttga tgctgttcct
     1681 ggtacatggc aataatcgtt gttataccag ttgccataga aactgatgat attccatgtt
     1741 gcaccgccat cgtgtgtaat gtaggccgaa taaggtgtag tattcatgcg gatgataccg
     1801 tcgttgagtg agcggaattt tacggcaaat tgattatccg ttgcaatggg aacggtaatc
     1861 aaagaccagg tatgaccata atcgcttgat ttcagaatat gagatttatt ggtactgaac
     1921 cagatggtgc ttccaacaac agaatagtag ccggtaacac catattctcc gctaaccggt
     1981 gccggaatat tacctgaagg tactcgtgtc catgtcgtac ccccatcttc agttgtatat
     2041 aattcaaaat atccttcatt cggatcgccc atgcagaatc cggtgtcggc attccagaaa
     2101 tgaacgacat tcggaaatcc gttgggtgct gcaaacgatg ctgttgtctg cggattccag
     2161 gtggttccgc cgtctgatgt atgcagtatc acacctccgc cggtgcttgc attataggcc
     2221 ggaatccatg cattgttgtt gtcaactgcg caaatcatgc tcagtccgta acccgtgtaa
     2281 ccgccaacta catagggtgt ccagcttgtt cctccgttgg ttgttttggt gaattcctga
     2341 atattgtttg ctgtgtttgc tccgtcgtaa gcagtagccc aaacagtatt ggaatcaaca
     2401 acactgatga atttaatgcc gcgcgatgtg cttgtgaacg ctgtgttttg cagagtccat
     2461 tgagcgatgg atgcaaatgc gacaaatgat aaaactaaaa cagtaaataa tcttttcata
     2521 ggtaatgttt ttttacaaat ataaatattt tataagataa gtcaataaaa ttcttcctga
     2581 aaataccact tttctatgtt tgaaagcaat ccggcaggcc ggaacaattc aggctgggcg
     2641 tagtctgaaa tataaaatcc gttgggttga gtctggagac tttacccggc aaataaattt
     2701 ataaagacta cccccggcct gagatatcag caaaaaacat taaaaggaat aatgttccgc
     2761 tattttgata ataatgcggt taacttcatc ggcactcagg gcggccatga gttcggcttt
     2821 gaatggtttg aagtcactca gtcctttgaa atattgcgaa tattgttttc tcatttcaat
     2881 cacagccctg cgttcgcctt tcaggttgat tgattcaagt aaatgacggc ggcaggtggc
     2941 tgttttgtgt gcaatcgtaa cgaccggaat agttcctcct tgcaatagag ccctgatctg
     3001 accaaaaatc catggatttc caatcgctgc acgaccaatc atgagaccat cggaaccagt
     3061 gatttttatt gcttctaacg ctttttccgg actattgatg tcgccattga caataaccgg
     3121 aatctgtaaa actgctttga cagcagcaat gggattccag tcggcggttc caccatattg
     3181 ctgtgcacgc gttcgtccat gtattgccaa tgctttgact cctgcatctt gtagtcgcag
     3241 agctgcttct tgcacaacaa tattctgtgc atcccagccc agccgggttt tggcagtaac
     3301 cggaatttgt accgcattga cagttgcagc tgcgatttcc tgcattagtg gcagattgcg
     3361 gagcagggca gcaccgtttc cttttgaaac cacttttttt acgggacaac caaaattcag
     3421 atcaatgatg tgtgcaccgt tttcttcggc aacacgagct gcttcggcta ctgattcggg
     3481 tttgttgccg aaaatttgca cccagcgcag ttcttcttca tgtgcggcat ccattttccg
     3541 aagacttttt tcgatattgc gtataatggc ttcggaggaa ataaattcgc tgattcccgc
     3601 atcagctcct tccgaaaggc aaatcctgcg aaagactgta tctgtgattc cttccatggg
     3661 agccagaatg agcggtgtct gtatgttttc gaggccggga agattcatct ttcttttttc
     3721 aatttcatac atgttgcagt cgtgcaattc atcataatga tgattaaaat gcaagtgttt
     3781 taagagtatg caaagttacg aaaatagtgt tctgatatat gtattggctg tttgtgccag
     3841 aagcgttgtt tttgttgata tagtctcttg tgaggcatcc tcatttccaa tcggcctttg
     3901 tttttttcgt tactctgtga accgaagtgc atgaaatgcg ctgagctcat gcgatgtcct
     3961 gagattgaag aagggagcac cttgctaaag tgatttaatg agtgaataaa ctgcgattca
     4021 agatgtcttt tgcatgtatt aatgcgtttg ccctgatgag tatgatatgt cttttgctgc
     4081 atgctttagt aattaatata tttgcattgt caaaacaaac agattatgca aagtccactc
     4141 aagggcaaac atccggctat tcagctcatt tcttttgtag gattatcgct tgccggcgtg
     4201 attgcagtga tgtttatcgg catgcttgcc ggggttttca tctggggtcc tgccttcacc
     4261 gattcgtttc tggttgcagg aaaaggtgaa gattatatcc tgcacatgaa atatcttcaa
     4321 atcctcagtc atttgggaat gtttatcgtt ccggcttttg tttttggctg gcttgccggt
     4381 ggtaacgcca tacgttattt ttcgctgaat agaggcatga gtgttccggt ttttctcgtt
     4441 tccattgctt tgattttttt atctcagcca ttgataaatc tgcttgctga gtggaacagc
     4501 acattatcgt tgcctgaaag tttgagtgga attgaatcat ggatgaaaga atctgaagaa
     4561 caagccaccg cattaaccgg tttatttatg aaagacaccc tttggagcag catggttttc
     4621 aatttattga tgatggctgt tattccggct ttgggcgaag agcttgtttt tcggggtgtt
     4681 ctgcagcgtt tgttgattaa atggtttcgg ccggcatgga tatccattct tttcacagcc
     4741 attattttca gtgcgtttca tatgcagttt tatggatttt tgcctcgtct gattctgggt
     4801 ctgatattgg gttacgcttt ttatgcaact ggccgtttgt gggttccaat cgttattcat
     4861 ttcatcaaca atggaatggt ggtagttgta acatggctct atgagcttgg tttcaatcaa
     4921 atgaatccgg atactttcgg taatttcaac gacaaccctc tttttcttat aatatcttta
     4981 ttccttttta ctgcatcgct tgttatcttt gcaaaacttg ccaaaaaaca cagatatgaa
     5041 caaagcgcag ctatataaca gaatttttgt tcagctcgaa gatttactga aaaaaagtcc
     5101 ggcgcctgtt gcctcgatgg ctacggtttc ggctgttttg tttcataaac tcaagcatta
     5161 ttcgtggtgt ggattttatt atttaaacag cggcgatttg attgttggtc cttatcaggg
     5221 tcctgttgca tgccagatgc ttgcgcgcca caaaggcgtt tgctgggcag ccattgatac
     5281 ccgaaagtca caactggtgc ccgatgtgga acaatttgct ggtcatatcg cttgtgattc
     5341 acgcaccaaa agcgaggtag tggttccatt gcgcaatgcc tacggcgagg tcgtcgctgt
     5401 atttgatgtt gatagtcatg tattgaattc gtttgatgcc gatgatgtgc aaggaatcga
     5461 gaaaattatg caattacttg aaccatttga aatatgataa cacttgttct ggcgcttctg
     5521 gctattggct ttttgcagat ttcggtaacg tatcgtcatg cattgctttt ttccaaagcc
     5581 agtattggta agttgctcct tgtttcattc ctgcttacga tcatgaataa tctgatggcg
     5641 tgggtagcgt tgttgtttac caatgccatc cgcgatacca tgtttttcga cagtacttat
     5701 gctgccatgt ttatccttgc aattcttgct tttaaggcct atttcaatac acgtagaagc
     5761 aaaattgctg ataacgtttt tgatattaca caaccaaagt tgcttgtttt attgggactt
     5821 gccacatcct tcgaggtttt tatcgctgta atgggtgctg gaatgattga aacagaactg
     5881 gttccggcat tgattattgt gcttgctgtt tcattgtttt tttccatgct tggatttctt
     5941 gcaggccgcc gcccaagcca gattacatct atacgggttt ttattctgat tgcttcggtg
     6001 ttttatttgc tggcagcttt gtcaaccata tactttttat tgtaaatttg tcgctttgtc
     6061 gctgaaaaat ccggctttat gaaaatacga atttccaaag aattttcctt cgaaatggct
     6121 catgcgcttg atggctatga tggcttgtgc aggaatgttc atggccattc atatcagctt
     6181 acagtgactt tgaccggaga gcctgaatcc gatattcaat cgccgaaata cgggatggtg
     6241 atggattttg gtgatttgaa aaaaattgtg cagcaagcca ttgttttgca gtttgaccat
     6301 gcttttgttg ttcgtaaagg gacattgagc catcttgaca aaaacgagat gttttcacgt
     6361 ttaatagaag ttgattttca accaaccagt gaaaatttgt tggtgcattt tgcttctttg
     6421 atcaattcaa tgctaccgtc gagggtgtct ttgtattctt tgaggttgcg cgaaacaggt
     6481 acttcctatg ctgaatggtt tgcttccgat aatctgtaat tcatgaaaac ggcctattat
     6541 ctgcgaaaaa tgcgcatgaa gctgcatgag aatatttcgg gcattaaacg gcctgcagta
     6601 cgatggtcgt ttattgggat tcttgctgca tcaatgattg gtttttttct ggtgctggct
     6661 gaggttggtt ttgatactga ttttgtttta cgcgagtggt acaacgaaaa aatcgaatgg
     6721 atcatcaatt tccttatggt gatgtattcc attcaggttt taatggtttt attgccgggg
     6781 ccgcctggta tcactccatt gaatattatt gtcaaaatca ttcttcttgc attgctgagt
     6841 ttgcattttt tcgattttat tccgttcgac atctcccttg aaagcacatc gggttacaaa
     6901 ctcgaatggc ttttcaatta cggactggtt atcatcatca tgtttcgctc ctggagccgg
     6961 attgtgaata atatttactc cagtaatata accagtgaaa agctgtttat tgtttctttt
     7021 atttctctga ttgctgttgg aacaatcttg cttttattac ctgtttctac taccgaacag
     7081 atttctttta ccgatgcttt gttcaccagc acttcagctg tttgtgtgac aggattgaca
     7141 acacttgaca ctgccactga tttcacatgg tttggcaaaa cggtcattct gttcctgttt
     7201 cagatgggtg gtatcggggt gatgactttt acctcgtttt ttgcgtcggt tggtcgctcc
     7261 aaacacagtt tatccgaagg aaatgcattg acaagcatgg tggtggccaa aaacatgcgt
     7321 aagcttttca cagctttata tgcggtaatt attgtaactt tcatagttga gtcatttgga
     7381 actgttttaa tatacctgca gcttccgccc gatgaatttc ccgataatat cagtcgtttc
     7441 acctttgcct tgtttcatgc tgtatcttca ttttgcaatg ccggcttttc tttggcaccc
     7501 aatggtttga tgaatgatat tttttaccat gaatggggca ttcagatgac gatggcctgg
     7561 cttatcattt ttggcggtct gggttttttt attattacaa actatatgga acgatttgcg
     7621 ctgggaatgc gtaatttcat caagcatacc attttgggac atgccagcaa gtttgaaccg
     7681 aatctgatta atatcaattc ccgtttggtt gtcaggacta ctttctggct gctcttgtta
     7741 ggtaccggat tgtttctgtt ttttgaaaat gatgcacttc ttagcgggct tcgttgggat
     7801 gaaaagttgt acaccgcatt tttcagtact gttacagcca gaacggcagg ttttaacacg
     7861 ctggactacg aaagcatcat gctgccttcc actttattga ttattattct tatgtggatt
     7921 ggtgcttcgc cgggctctac cggcggtgga ataaaaacta ccacttttgc gattgcattt
     7981 cttactgcct tttctttggg tcgtggaaaa acaaccactc attttcacaa tcgcgaattg
     8041 agccgtcaat cagggcgtaa agctcaagtg ataattttct tttctgtttt tgctatttta
     8101 attgcaacca tgtttatggc ttggtttgaa cctggtgttt cgatgcaacg tttgttgttt
     8161 gaggcgtttt ctgcttttgg aactgttgga ttatcgatga atctgacaat gtttctgagc
     8221 gaggaatcaa aattcattct tattatgctc atgttcatcg ggcgtatcgg cataattaca
     8281 ctgttcgttg cttttatcag gaaaaccaga ttcctgctgt ttagttaccc cgaagaagaa
     8341 ttgtttattt aaaaaattgg tgctatgaaa ataatggtta ttggtcttgg taatttcgga
     8401 actgcccttg ccatcaagct tacaagactt ggaaatgaag tgatcggcat tgaccgcagc
     8461 cgccataaaa ccgaatctat caaggagttt gtaactacgg ttgttactgt tgatattacc
     8521 gaagcgtcgg ctgtagcgca gttacctgtg gccgaaatgg atgtgattgt tatcagtatc
     8581 ggtgaaaatc tgggcgacag tgttattgct actgcattgc ttaaagataa tggcgcaaaa
     8641 cgaatctgtt gccgggccat ttccgaaaca catttcagca ttcttatgaa tatgggggtt
     8701 catcatattt ttacacctga agtcgatgct gccagcaact cggtcagtac tgttttgtat
     8761 gagctggttc ataccgccta tgtcattggc gatgattata aggtgtgcga gatttatgtc
     8821 ccaaaacgtt ttgtgggcct gaaagtgaaa gatctgacac tgaattcaca tggtttgaaa
     8881 ctgttgttgt ttcggcaggc caaaagcgaa ttacatcctc agtacatggt gaaaatgatt
     8941 ttcgaagaca gagtaaagga ttacgatgaa atcataatga aaaaagatga tttattggtt
     9001 ctctttggcc tgaaaaaaga tttggatcgc tttgttgctt tatagcttgc tgtaattttt
     9061 tcggatttca acaaaaaaag actccgtaat tcacggagtc ttttttgcga tggtatctga
     9121 aagtttttat accgtgttta catataaaat agtccaaaat gaaaatcaga ttattgcgta
     9181 atttgatttt tctaacggca taatccaaat ttgaaattgt agtacgtctt tggactatct
     9241 acaatttcaa tttggtatta ttgtcttgct tttttcgcac tgacatttct tttctttcct
     9301 ttagtgatga tttgtttgga atgatcatcg gttcctgttg acaaatcaat ggtgttcttt
     9361 gtagattcag ttgctgcttc ttttttcagc aacagtggtg catcggggaa gcgaacatcg
     9421 gcatttttag aataggtccc attgtctctc atagcatctg cacggtttgc aacaatactc
     9481 cacgaaaatt cgatattgct ttgaccattg ctgagttcca caacgtcaaa tccggtttgt
     9541 gtcttgttgg ttacataaac gccattgcat tcaccttcga gttggataaa aactttgagt
     9601 ggatgattgg cgtcaaccag aatattttgt accagaattg gatcgagttc gatatgagca
     9661 cgtccattga ccagcttgcc tacaccatag tcctgaaaaa ggatttcggg tgcttcggtg
     9721 caaaacatga tggctttggt gttgtcgggg cgagaaacta tggttgctgc tgttccgcta
     9781 ccactgattt taatatcagt tccaccggtt gttgtgcctc ctacataggc ataagcgccg
     9841 tatgaattgt cgaaatatcc accccaggta tcattggctg agttgtgggc tgtgccgtaa
     9901 actccgatag tggcacctgt aaatgcgccg ccagagccct ctccggtgag ggtatatgct
     9961 gacagattgt tgcctgctcc aagtactgca gttccggtag tactggttgc cttactgaag
    10021 gatccgctgt ttgttcctgt aaatgcacca ccagatccat cggtaagata tgcaccgctg
    10081 gcattgttgc ctgtgcccat gacacctgtt ccatttgtgt gggaattgta tccgtaaaca
    10141 cccaaacctg caccctgagc ggttgttcca taaacaccat caccagtcgc tgttccggtt
    10201 gcagctgagt taacacccat gatgccaatg ccgtcatcgg ctgtaacacc tgctccaatg
    10261 cctttgggtg ttgtggtttc gtatgttgag ccattgatgg ccgacaaagc tgagtttgca
    10321 taataggacg tgctgttcag aaccgacatg atgcttgcac atccatattg gttcgaagag
    10381 gcataaatgg cagttcctgt tccagtgccg gctgtagcgg tatttacagc ataaagcgca
    10441 tcgcctccgg cagctgcatt ttgtgtttgg gttgttgcat ataaagcact attattcgta
    10501 gaaagtactc cgcgtgtaaa agtgcttcct gatccccaga cgcctgctgc accggtttgc
    10561 atgctttctc cataaacacc tgaaccgagt ccggctccgc ttgttgcaac attgtaacca
    10621 gacacaccat cgaatcctgc ggcttcggtg tatcctatgg ttcccgtggt ataggcatcg
    10681 ccgtcaccaa aaacacccga tgcgcctgtt tgaaatgttg ctccatatac accacaacca
    10741 ctacccgcac ccgaggccgt tgtgtttcct ccataaacgc cggttcctgc cgcctgattg
    10801 gtatatccgc tgatggcatc aggaaatgtg gcattgccct gtgcctcgaa cagatcggtt
    10861 gcatacaatg ctgtggtgcg gtttacaagt acattgccag cactcagaat ccgtgccctt
    10921 tctgtattgt ttgttcttgt aacaaaatca acgccatcgg tggtgccgat aaaattgctt
    10981 gttgcaacag tccctgcatt gcccaccagt ttccatgcgt cggctggcat ggctcccgat
    11041 agcaggcgaa cccatttgct gctgtcccaa taataaaatc cgggcgttac atcacccgct
    11101 gttgcagtat tgtaaaccaa caaactggtt gcgggtgtag ttaccggagc tgcaatcaag
    11161 gttgacgaaa gcgtaacccg tggaaccaat actccgctgt ttgttgatct aacctcaagc
    11221 atggccgagg catctggtgt tatacttact gatgatatac cgacctgcgc catgcttatt
    11281 cctgctgtca gcagcattaa agcgaaaagg agaattcttt tattcatgat ttttcatttt
    11341 ttttacgcta cctgaaatta tatagcagca aaattacgaa aaacattata actgtaaaaa
    11401 aaaattgtag gtaggcaaaa aattattttt aatagtaaaa agtaaatttt atttaaaccg
    11461 aatcattata atttgttatg aattatttgt ggtcttgtat atttaaaaaa tgatctgaaa
    11521 ggatgtggaa ttatgatttt tggcagacac tttttttgct tcctgggttt gtttttttaa
    11581 aaaagcattg ttttgtcagg gtatgtatta tttttgcacc cctgatttac ttgtatgact
    11641 atgattcgtt tcattttttt attgatgatt tccgttttgt ttgtggcttg cggacacaat
    11701 aatgaaaaaa gtgttccagt tgcagatgat tctttgacag ttgcccagtc gaaattggtg
    11761 tatgatactt cacttacatc ggtggcacag ctgatggctg gcataaagcc agatgatact
    11821 tccgggaaag cggccattgt gcaaaaagac gtctgggaaa atcatgctga ttcaataagt
    11881 ttgctgtttg aaaatgccga aaaaaagaat cttgttttca tgcggcagtg ggccgctgct
    11941 gaattacccg aaggaaaaga cactgcggct acattgcttt atgccttttc tggccccgat
    12001 tatttgtatg ccaatacatt tttcccgtgt gcaaaaaaat atattttgtt tggtcttgag
    12061 cccgtagggc ggattccggt acttgacaca tcaaagaatc agacactttt tttcaataat
    12121 atccgtcggg cgctgaaaac ttcattgtcg cgtaattttt tcatcacttt gcatatgagc
    12181 ggcgacctca gggcaagtga atacaatggt gttacttcca ttctgatgct ctatgcggct
    12241 tataccggcc ataaaatcaa agatatttct tatgtcactg ttgatacaaa cggtcatgtt
    12301 cagccgtgtt catacgattc gttgaacgtt tgccatcaga cggctggtgt ttgtctgaat
    12361 ctgattgata gtcttggtcg ttacaaggaa ctcacttatt tttcattcaa tgcagagaat
    12421 tctgcctttg aaaaatctac cgtaaagaaa tatcttgaat ccctgaacgg gaaaacgtat
    12481 ggcatgctga aagccgcatc atatctcatg cattacgatg gtttcatgaa aatcagagaa
    12541 gtgtttactg caaaagtttc agcggtactc accgatgata cagggttgtc ataccggaca
    12601 ttgaaagctg tttttccgaa gctacagctg tatggaaaat tcagtcagcc ggttgacatg
    12661 ttcaactata taaacctccg tgatctgcag ctggcctatg attcattgca ggccaaaccg
    12721 atacggttta cttatggcta cggatatggc caaaaattga tgattggccg gaaataaaaa
    12781 aagccggcca tcatttctga agccggctga taatttcatt gcaggtatca ggcatacact
    12841 ttcacatcag cttcgccttt gaggaa
//
LOCUS       DAOPKE010000080        12509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5315_length_12509_cov_11.622772, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000080 DAOPKE010000000
VERSION     DAOPKE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_12509_cov_11.622772"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..836)
                     /locus_tag="PKY63_09800"
     CDS             complement(63..836)
                     /locus_tag="PKY63_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00948.1"
                     /translation="MSTFDYVISLIPFAGSTFSAIMFLWLYRSNNKTHQIRLSLFFMF
                     LALFYLMSALFYSGLQQIRPVLYVLSLPVTLSILPSFYIFLTSITGRYNKHSVRRHIH
                     YLPTIIFFWFLMLFWFEPASVQYNYLSGKLNDISDLPMLTFIRKVYQIGVYAVIHAQF
                     VLYFILFSIELRQSKRQIENNFSLNEQINLSGITYFVLFFVLLYILILFSHFLGVSIY
                     PASRIIFNILSSGIIIFLLFRGLKFSTMTDAEDNETNQD"
     gene            complement(1171..1539)
                     /locus_tag="PKY63_09805"
     CDS             complement(1171..1539)
                     /locus_tag="PKY63_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00949.1"
                     /translation="MRRTNYSIWPVMLLATITMVLMLIIKPAILTEAELRLDSPIPKN
                     PGTTNAGNSIWSSGNKINFNAFGAKNHQTATRKSTRKKLRVLLLQLPNITADNKGKRH
                     DQQFIKSSSGLCPGAYEHAL"
     gene            1893..3449
                     /locus_tag="PKY63_09810"
     CDS             1893..3449
                     /locus_tag="PKY63_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF00950.1"
                     /translation="MRLYLTAFICLLTFLGNSQNKYWVFLSDKDTEGYDPYAFFDSKA
                     IERRILNDIPVWDYSDVPVNQQYVESLSSLCDSVSYASRWFNAVCLYATDYQLDAIRR
                     LPFVIRIEHAGSGHFVTAQDLSEPDSYIRSPGLQSKQLSLMQASLFREAGITGKGVRI
                     AIFDGGFPNVDVLPCFKHVRDNNRIIKTWDFTRNKENVYGFSSHGTTVMCCIAGIDTA
                     GDPFGLATDAEFLLARTEVNAEPYSEEENWVAALEWADQNGADIVNSSLGYTFHRYFP
                     YEMDGKTSFVTRGANMAASKGILVVNAAGNEGDGKWKYIGAPADADSVLSVGGVDPYS
                     GYHINFSSFGPTADRRLKPNVCAAGQVYAYGKKGPGVHYGTSFASPLTAGFAACAMQA
                     KPGVRTYDMFLEIQKSGHLYPYYDYAHGYGIPQASYFLNTAAEESLSEIEISVSDSEV
                     IVAVAVDPTDSTENNYCYVHCSNASGVLSWYKVLTVYEGEILRMNKNDFRPGEILRVY
                     YDGKIKSLQF"
     gene            3460..4164
                     /locus_tag="PKY63_09815"
     CDS             3460..4164
                     /locus_tag="PKY63_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00951.1"
                     /translation="MKKISFSFLFLLMASVLMAQYPVLDESVTPYDTLRPETGPNGKH
                     FFNSFVGWHFAIPYDATDAAPVKPFSSSGLRVGWMWKHRISNFLSFGYLLDFNTYGFS
                     YSQSEEKTFPDSLVHESQSIGLTAIGTGLYLRFNFDTKRGNFIGHYLDAGAYADWIPG
                     RTLATKDEIDFDQIQRSRISRLHYVEKYQYGLYAAIGLNKFTIFGRYRISDVIKAPYQ
                     SAELPRLSVGIGLCLF"
     gene            complement(4206..4586)
                     /locus_tag="PKY63_09820"
     CDS             complement(4206..4586)
                     /locus_tag="PKY63_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00952.1"
                     /translation="MKLIMFFIAAFATMASLNINAQNTFKISLAGQTTDTVSRQEITA
                     NPIVAVIGVSQAAEFAVESFEFTYMNSDGDLITIATKSNRLNDGMLSALKNTSLKKIY
                     LENVLFTLNGETRPAASTRFYLKD"
     assembly_gap    5165..5264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5468..6097
                     /locus_tag="PKY63_09825"
     CDS             5468..6097
                     /locus_tag="PKY63_09825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPF00953.1"
                     /translation="MGITEKIALWATAFIASTGYVGIFVLMVMESMVLPVPSEAVMPF
                     AGFNIVDGNLTWAGVIIASTLGSIVGSLISYYIGYYGGKPFIARFGKYLLLNQHHLEL
                     SEKFFAKRGEITILIARFIPIVRHLISIPAGFGKMNIPKFILFTTIGAGIWNAFLTWV
                     GFVLRENWSEVMKYSHVIDIVVLVVLGGAALFYLYKIIKSRKTQKNSAE"
     gene            6117..6380
                     /locus_tag="PKY63_09830"
     CDS             6117..6380
                     /locus_tag="PKY63_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00954.1"
                     /translation="MKISVDISYYPLTENFKIPIKKFIAQLNSYSEISVKSNGMSTQV
                     FGEYADVMRIVTKEMEMAMELPHSIFILKMANATLDNDYNPNK"
     gene            6377..6979
                     /gene="pnuC"
                     /locus_tag="PKY63_09835"
     CDS             6377..6979
                     /gene="pnuC"
                     /locus_tag="PKY63_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPF00955.1"
                     /translation="MSEILNYIVANWFELTAAALGVIAVFLQIRINSSYWVVSILNVS
                     MYIWVYISQQLYALMILMVYYVAMSVYGFYTWRFGARYKNRNHKVKISYTKLRTWIFT
                     VITVFASFMIIAWALYQFTDSRTPILDGLVTALSFSATWMLARKKIENWLVWLVADII
                     SCYLYYSQKMYPTLVLYAFLSVMAVIGYYSWKNKMKQVNA"
     gene            6984..7505
                     /locus_tag="PKY63_09840"
     CDS             6984..7505
                     /locus_tag="PKY63_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF00956.1"
                     /translation="MRIAITGPESTGKTTLTEYLSAHFGGVAVSEFAREYLENIGRSY
                     NYDDILHIAQQQILSEDRASMKSKIVFCDTELLVTKIWCDDKFGRCHSWIIEELQKRD
                     YDLVLLCNTDMPWEPDPQREDPDRRDYLMGLYRNQVANYYVNVVEISGDGDERLKMAC
                     SVVSEFLKTKSCL"
     gene            7502..8095
                     /locus_tag="PKY63_09845"
     CDS             7502..8095
                     /locus_tag="PKY63_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00957.1"
                     /translation="MKTSFVSFFLIVAVSFLLVSCGGKNRSGSGEINDSAAIENECEK
                     ETSLSLFVDKKDLKDNRAFVTADSMFKINKQQFVFTNDSSAEIQWAGISKMASGAEDE
                     VVVHVALYAKNGKTLRPGLYKNMDFDSDYFAKVNFETDAGTIWFNWASGMPDPGAVKI
                     HQIDRNSVCGSVHLNVDKPDNNSIGVVKLNGNFYIEN"
     gene            complement(8123..8563)
                     /locus_tag="PKY63_09850"
     CDS             complement(8123..8563)
                     /locus_tag="PKY63_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00958.1"
                     /translation="MKKLCFAFLLFIPALFTNAQETTYQAVIDNFFSIYKNDGPEKAI
                     DYIFSTNPFLEPNSEQVTAIKLKLATAVAVIGDYYGFDPVSVKELGPAMTIGTYMVRY
                     KRQPLFFTFILYKPDKIWQIQTLRFDDKIEALQMNDFENKAATK"
     gene            complement(8564..9454)
                     /locus_tag="PKY63_09855"
     CDS             complement(8564..9454)
                     /locus_tag="PKY63_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF00959.1"
                     /translation="MFQYVVFIFMQTLIRVMPFWLMYGLCNVNAFLLRHVIRYRRKVV
                     QRNLHASFPEKDDKELNRIERQFYRYFADMLFEAGKGYKMSRRALAKRYKVNNPELME
                     QFYKEGKSVVLDLAHFNNWEWTTGVFGSAFQHKVFAIYKPMRNARIDAFIRRSRERRN
                     YHVVPLKNTGLMFRNMNQQPCAFIMASDQSPVQLDKAYWVEFLHQTSGFLHGPETYAR
                     KFGLAVIFTQVLKIKRGYYEINLIPITETGKDLPEGHLTARYAQLLEETIRQQPSRWL
                     WSHKRWKKTKPADKEIIKIQ"
     gene            9577..11049
                     /locus_tag="PKY63_09860"
     CDS             9577..11049
                     /locus_tag="PKY63_09860"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPF00960.1"
                     /translation="MKHVYTLFFLCTVFLSQRVFSQEMWGVVNSNYAGINSNLINPAG
                     MVDSKYWLDINIVTLDVFAENNSLYVPHKELFFLEILSPAVDFGDETPVLKDTYDKQR
                     NLHANFSTRLELPSFMLCKGDQAFGLQMSVRGAGSTRGIEYHTSKFIYEGIDFDPQQN
                     EFYELRKMKTSALSWGEIALSYSKAFTTFSSTYMAIGATLKKTMGLGGAYFNAPYANY
                     IVYDDSTLAVEELDAEFAAAMPMDYSNNEYSSDQGMFLGGGWAVDLGFVFQKKRDGNG
                     FMRVRQLCAQKWSSYKYKFGISILDLGYVKFRDNAFVYSYDDAQTYWPGFDRFDPQSV
                     DGFRNEFNYHFAHNTEADAREFTIGLPTAVSLQYEWHPVGYWFFNTSFVYPMPVFKNS
                     VIRPTQLSFTPRFEKRRFELSIPLSVYEWSRLRAGLALRFWNLTIGTDYFSSWTGWFD
                     FYGSDIYVSWKMSFAKGYCKKGQSKFHKGKRYYKDACPDF"
     assembly_gap    11203..11302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11303..12387)
                     /locus_tag="PKY63_09865"
     CDS             complement(<11303..12387)
                     /locus_tag="PKY63_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00961.1"
                     /translation="MNTCKTSLSLIAFAIIAAYLIISNFVFFPNQIISYDIYGYYLYL
                     PQTFIYNDLSIHNPAQVLSLMEKYGSSSTFYQATLLPDGTYVMKYSMGLAILYSPFFF
                     IAHTLAPALGYAADGYSFPYQFSLLLNGIIYSIAGFWFLRKVLLKYSSEKVTAIALLI
                     IVAGTNIPVHMAMYGQNAMSHNYLFALYCMALWFTIRWHETYKVKFIVFLGLACGLAI
                     LSRPTEIVILAIPALWSVDSWKALQEKFKTIFNHFGQIAVFGFIVLALGFCQLLYWKL
                     HTGHFLYYSYGSNPGEGMEFFSPFIANVLFSFRKGWFVYTPLMLLACTGFIFVYKKNR
                     KIFWSLLVYTCVNLYLVSCWSTWWYAQS"
BASE COUNT         3464 a         2355 c         2694 g         3796 t
ORIGIN      
        1 aaagccaaga ttgaagtggt agatgttgat atttctcttg tgattgatca gatacgaaag
       61 cttcaatcct gatttgtttc attgtcctca gcatcggtca ttgtgctgaa ttttaaacct
      121 ctgaataata agaatataat aattccagat gacagaatat tgaatatgat gcgagaagct
      181 ggataaatgg atacgccgag gaaatgtgaa aataatatca ggatatatag taaaacaaag
      241 aaaagtacaa aataggtaat acctgataga ttgatttgtt cgttgagtga aaaattgttt
      301 tcaatctggc gtttactttg cctcaattca atcgaaaaaa gtatgaaata taaaacaaat
      361 tgtgcgtgta taactgcata aactcctatc tgataaactt tcctgataaa ggtgagcatg
      421 ggtaaatcag agatatcgtt cagctttccg gataagtaat tatactgaac cgaagccggt
      481 tcaaaccaga acagcatgag aaaccagaaa aaaatgatgg tcggcaggta atgaatatgt
      541 ctgcggactg aatgtttatt atagcggccg gtaattgaag taaggaaaat gtagaaagag
      601 ggcagaattg acaatgttac tggcagggat aaaacataca aaaccggcct tatttgttgt
      661 aaaccactgt agaacagtgc cgacatcaga tagaacaatg ccagaaacat aaaaaacaaa
      721 ctcagccgta tctgatgtgt tttgttgttg ctgcgatata accatagaaa catgattgcc
      781 gaaaatgtgc ttccggcaaa aggaatcaga ctgattacat aatcaaatgt tgacattgga
      841 ctgttttctg accggcaaag atattattaa agaatttctc cgcgaaaaat tatatcatta
      901 tctgaggtgt atatttcatc cgcaaatttt ccgtatgctc cttttgcgac ctcctcgtga
      961 agtcatgttt atattaggta aacaataccg gcaaaatgtc tttgaaatcc aaaaccggat
     1021 gaatcattct tgttgtaatc ctgtttcgac attaaccgaa agccgttcca gcatattatt
     1081 gtgggatatt tgtttgcatt tacacctgat aactatgcaa cgggtaagga tgtaggtgac
     1141 gcacgaaaac aaaatgggct tttcctgact ttacagcgca tgttcataag cacccggaca
     1201 tagtcctgaa cttgacttga tgaattgctg atcatgcctt ttgcctttgt tgtctgcagt
     1261 aatattgggc aattgaagca gtaaaacccg tagtttttta cgtgtgcttt ttcttgttgc
     1321 tgtttgatga tttttcgccc caaatgcatt gaaatttatt ttatttccgg aactccatat
     1381 actgtttccg gcattggttg ttccggggtt tttagggatg gggctgtcta aacgtaattc
     1441 agcttctgta agaatggccg gttttattat cagcatgaga accattgtta ttgttgccaa
     1501 aagcatgacc ggccatattg aataattcgt tcgtcgcatg agaattttta tctttcgtta
     1561 gctgccatca aaattacttt taaatgtgat atattttcga tgtgatgctt ttttatgttc
     1621 atttttatgc caagggctgc agttagtcat tgtagtttga tttgtaaaaa gctgtttttc
     1681 attgcaatat attttacatt catgtgatat attttattgt ttttttgatg aacgggcgtt
     1741 tttattgacg aataagcagg actgtattct tgcaaaaaac gagcttaaag aacagttgaa
     1801 aaataactaa gttgcattta ttcagtattt tgattaagaa atccgtgttt tgtggctggt
     1861 tttttaaaaa tcagtatttt tacaaaaaaa acatgcgtct gtacttaacc gcctttattt
     1921 gtttgctgac atttttgggc aattctcaaa acaaatactg ggtttttctc agcgacaagg
     1981 acaccgaggg ctatgacccg tatgcgtttt tcgactcaaa ggccatcgaa cgccgcatcc
     2041 taaatgatat tcccgtttgg gattatagcg atgtgcctgt taatcagcaa tatgtggaat
     2101 ctttgtcttc tttgtgcgac agtgtttcct atgcatcgcg gtggttcaat gctgtttgtt
     2161 tgtatgctac agattatcag cttgatgcta ttcggcgttt gccatttgtt attcgtattg
     2221 aacatgcagg cagtggtcat tttgttacag ctcaggattt atctgaaccg gactcctata
     2281 ttcgctcacc aggacttcag agcaagcagc ttagtcttat gcaggcaagt ctgttccgcg
     2341 aggccgggat taccggaaag ggcgtccgga ttgccatttt tgatgggggg ttcccgaatg
     2401 tcgatgtcct tccctgtttt aagcatgttc gtgacaacaa ccggattatt aaaacatggg
     2461 attttacacg caataaggaa aatgtatatg gcttcagctc gcatggaact actgtgatgt
     2521 gttgtattgc cggcattgac accgccggtg atccgtttgg attggcaact gatgccgaat
     2581 ttttgctggc gcgcaccgaa gtcaatgcgg agccctattc cgaagaggaa aactgggtgg
     2641 cagctcttga atgggccgat cagaatggtg cagatattgt caacagtagt ctgggctata
     2701 cttttcaccg ttattttcct tatgaaatgg atggaaagac atcctttgta acccgtggcg
     2761 ccaatatggc agcatccaaa ggcattctgg tggtgaatgc agctggcaac gaaggtgatg
     2821 gaaagtggaa atatattggt gctccggccg atgccgacag cgttttgtcg gtaggtggcg
     2881 ttgatcctta ttctggctat cacatcaact ttagttcctt tggccccact gccgacagac
     2941 gtctgaaacc caatgtgtgt gcggctggac aggtgtatgc ctatggcaag aaaggccctg
     3001 gtgttcatta tgggacttcc tttgcctcgc cgcttactgc cggctttgcg gcctgtgcca
     3061 tgcaggcaaa accgggcgtc agaacttatg acatgtttct cgaaattcag aaatcggggc
     3121 atctttatcc atactacgat tacgcacacg gctatggtat tcctcaggca tcgtattttc
     3181 tgaataccgc agcggaggaa tccctgtccg aaattgaaat ttcagttagc gacagcgagg
     3241 tgattgttgc tgttgcagtt gatcctactg acagtacaga aaataattat tgctatgttc
     3301 attgcagtaa cgccagcgga gttttgtcat ggtacaaggt tttgacggtt tatgaaggcg
     3361 agattctgag aatgaacaaa aacgactttc gtccgggcga aatcttacgg gtgtattatg
     3421 atggcaaaat caaatctctc caattttaaa atcactatca tgaaaaaaat atccttctct
     3481 tttttgtttc ttctgatggc atctgttctt atggcacagt atccggttct tgacgaatcc
     3541 gttactccat acgatacatt gcgcccggaa actggtccga atggaaagca ttttttcaac
     3601 tcatttgtag gatggcattt tgccattcct tacgatgcta ccgatgcagc accggtcaag
     3661 cctttttcat catcaggact tcgcgttggc tggatgtgga aacaccgcat cagcaatttc
     3721 ctttcttttg gttatttact tgattttaat acctacgggt tttcgtattc tcaaagtgaa
     3781 gaaaaaactt ttcctgattc gcttgttcat gaatctcaaa gcattggact tacagccata
     3841 ggaaccggat tatatcttcg gtttaacttc gacacgaaga gaggcaattt catcggacat
     3901 tatctcgatg ccggagcata tgctgactgg attccgggac gcacgctggc aaccaaggat
     3961 gaaatagact ttgatcagat tcagcgtagc cgtatcagcc gtctgcatta tgtcgagaaa
     4021 tatcagtacg gattatacgc agccatcggg ctgaataaat tcaccatttt tggccgttac
     4081 cggataagtg atgttattaa agctccgtac caatcggctg aattgccgcg gctttcggtt
     4141 ggaatcggac tgtgtttgtt ttaaaacaat caggttttaa tgagtcaaaa gcttagtgag
     4201 tgatatcagt ctttgagata gaatcttgta ctggctgctg gtctagtttc tccgttaagt
     4261 gtgaacaaaa cgttttccag ataaattttt ttcagtgatg tgtttttaag cgcactcaac
     4321 attccatcgt tcagacggtt gctttttgtt gcaatggtaa tcagatctcc gtcggaattc
     4381 atataagtaa actcaaaaga ttcaactgca aattcagctg cttgtgaaac accgattact
     4441 gctacaatcg ggttggcagt aatctcctgc cgcgaaacgg tgtcggttgt ctgtcctgca
     4501 agagagattt tgaaagtgtt ttgtgcattg atgtttaatg atgccatagt ggcaaatgct
     4561 gcgatgaaaa acatgattag tttcataata ttgaggtatt ttgagacaaa tataaacaaa
     4621 tattttaaaa tccatgggtg tcttaaaaca aaccgcacca atccaacact ccatagttct
     4681 accgatgggt gtagtcttaa gaaatgaacg gttgggttta gtctccaaga ctaaaccctg
     4741 cacaaaatat ttaaagacta caccctgcgc agaacatgga atagttggta agaggtctca
     4801 gcatccgaca ttttggcgtc cgcttgtctt ttttttgagt gaaagagcgg acacggaatc
     4861 gtcgggttca agaggtctca gcatccgact cttccaggtc cttcattgca ttgtggactc
     4921 tggaaggtcg gttcgctttt cgaggttgtt ttttttgctg cagtaacttt tgagatagtc
     4981 cctgtcacag aatctaccgg tcaaacgaca accttttatt gtaaagcact ttatcgtcgg
     5041 caaccagctt caggtaatat accggatcgg aatacacctc agcatcgtat tcaaatgtga
     5101 gtatctgatg tccttcgacg ggaccgttgt acatttcgcg cagtaaaatt ccgtcttcgc
     5161 tgtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcagt aatgctgcaa
     5281 agatgatggt tctcatggct caggtgtttg ggtgaataat cggctcaatg aaatataaac
     5341 gtcggtgaaa atacaattat tttaatcggg agaaatattt tttttgcgtt tgatatttgt
     5401 cagatttcga catttgcttc gtctgattat tgaattgttt ctaactttga gccataatat
     5461 cagaaaaatg ggaattacag aaaagattgc gttgtgggct acggccttta ttgcttcaac
     5521 aggatacgtt ggcatttttg tattgatggt catggaaagc atggtacttc cggtgcccag
     5581 cgaggctgtg atgccgtttg ccggttttaa cattgtggat ggaaatctga cctgggctgg
     5641 cgttattatc gcatcaaccc tgggcagtat tgttggctca ctcatttcgt actatattgg
     5701 ttattatggt ggtaaaccgt ttatcgctcg ttttggtaaa tatctgctgt tgaatcagca
     5761 tcatcttgaa ttgtcggaaa aattctttgc caaacgtggt gaaataacca ttctcattgc
     5821 gcgtttcatt ccgattgtgc gtcatcttat ttcaattccg gccggattcg gtaaaatgaa
     5881 tattccgaaa ttcattttgt ttacaaccat cggagctggt atctggaatg cttttcttac
     5941 atgggttgga tttgtccttc gtgaaaactg gagcgaagtc atgaagtatt cccatgtgat
     6001 tgatattgtt gttcttgtcg tacttggcgg cgctgcattg ttttatctgt acaaaatcat
     6061 taaaagcaga aaaacacaaa agaattcagc tgaataattt ttaaacacaa aatgtaatga
     6121 aaatcagtgt cgatatttca tattatcctt tgacagagaa ttttaaaatt cccattaaaa
     6181 aatttattgc gcagttaaat tcttacagcg aaatttcagt gaaatcaaac ggcatgagca
     6241 cacaggtttt tggtgaatat gccgatgtga tgcgcattgt tacgaaggaa atggaaatgg
     6301 ccatggaatt gccgcacagc atttttattc ttaaaatggc aaacgctacg ctcgataatg
     6361 attataaccc aaataaatga gcgaaatcct gaattatatt gttgccaact ggtttgaatt
     6421 gacagccgct gcattgggag tgattgctgt ttttctgcag atacgcatca attcttcgta
     6481 ttgggtggtg agcattctca atgtatcaat gtatatctgg gtttatattt cacagcagct
     6541 ttatgctctc atgattctta tggtttatta tgtggccatg agtgtgtatg gcttttatac
     6601 atggcggttt ggagcaaggt acaaaaacag aaatcataaa gtgaaaatca gttataccaa
     6661 actgcgcacc tggattttta ctgtcatcac tgtttttgca tcttttatga ttattgcctg
     6721 ggctttatat cagtttactg attcccgcac tccgattttg gatggcctgg tgacagcatt
     6781 gagcttttcc gctacctgga tgctcgcacg aaagaaaatt gagaactggc ttgtatggct
     6841 cgttgcagat atcatttcat gctatctgta ttattcgcag aaaatgtatc ccaccctggt
     6901 gctttatgct ttcctgagtg ttatggctgt aatcggttat tattcatgga aaaataaaat
     6961 gaaacaggtt aatgcataaa aatatgcgaa tagcgataac agggccagaa agcaccggta
     7021 aaacaacgct cactgaatat ttgtctgcac attttggtgg agttgcagtt tcggaattcg
     7081 ctcgtgaata tctcgaaaat atcggacgtt catataatta cgacgatatt ctgcatattg
     7141 ctcaacagca gattctgagc gaagatagag catccatgaa aagcaaaatt gttttttgtg
     7201 ataccgaatt gctggtcacc aaaatatggt gcgatgacaa atttggtcgt tgtcattcat
     7261 ggattattga agaattgcaa aaaagagatt atgaccttgt tttactttgc aataccgata
     7321 tgccctggga gcccgatcca caacgcgaag acccggatag acgggattat ttgatgggtc
     7381 tttaccggaa tcaggttgcg aattattacg tcaatgtagt ggaaatttcc ggcgatggcg
     7441 atgaacgtct gaagatggct tgcagtgtag taagtgagtt tttgaaaacc aaatcctgtt
     7501 tatgaaaacg agttttgtta gtttttttct gattgttgcc gtttcatttt tgcttgtttc
     7561 ctgcggaggc aaaaacaggt ctggtagcgg ggaaatcaat gactctgccg caatagaaaa
     7621 cgaatgtgag aaagaaacat cgctgtcact ttttgttgac aaaaaagatt tgaaagataa
     7681 tagggcgttt gtcactgcgg acagtatgtt taaaatcaat aaacagcagt ttgttttcac
     7741 caacgactca tcggcggaaa tacaatgggc aggcatttca aaaatggctt caggagctga
     7801 agacgaggtg gttgttcatg ttgcgttgta tgcaaaaaat gggaaaaccc ttcgtcctgg
     7861 gttgtacaaa aacatggatt tcgatagtga ttattttgcg aaagtcaatt ttgaaaccga
     7921 tgccggaacc atctggttca attgggcctc cggcatgcct gatccgggtg cagtaaaaat
     7981 acatcagatc gatcgtaact cagtctgtgg ctctgttcat ctgaatgttg acaagcccga
     8041 caacaactca atcggcgttg tgaagctcaa tggaaatttt tacatcgaaa attaaaaccc
     8101 cgtcgctgtt aaatgctgtc tattactttg tagcagcttt gttttcaaaa tcattcattt
     8161 gcaatgcctc aattttatcg tcgaagcgca aagtttgtat ttgccagatt ttatcaggtt
     8221 tgtagagaat gaaagtgaaa aacaagggtt gccttttata ccggaccata taggtgccaa
     8281 tggtcatcgc tgggccaagt tctttaacag aaaccgggtc aaatccgtaa tagtcgccga
     8341 tgacggcaac agcagtggcc agtttgagtt tgatggcggt aacttgctct gaattgggtt
     8401 ccagaaaagg attggttgag aaaatgtaat cgattgcttt ttcgggccca tcatttttat
     8461 agatggagaa aaaattatca atgacggcct gatacgtggt ttcctgagca ttggtaaata
     8521 atgcaggaat gaatagcaaa aatgcaaaac aaagtttttt catttattgg attttaataa
     8581 tttccttatc ggcgggtttt gtctttttcc agcgtttgtg cgaccaaagc cagcgggatg
     8641 gctgctgcct gatcgtttcc tcaagcaatt gtgcgtagcg cgcagtaaga tgcccttccg
     8701 gcaaatcttt tcctgtttct gtaataggaa tcagattgat ttcgtagtat ccgcgcttaa
     8761 ttttcaatac ctgtgtgaaa attaccgcca gtccgaattt tcgtgcataa gtctcaggcc
     8821 cgtgcagaaa tcctgatgtc tggtgcagaa attcaaccca atatgctttg tcgagttgca
     8881 cgggcgactg atctgaagcc atgatgaaag cacatggttg ctgattcata ttccggaaca
     8941 tgagtccggt gtttttcaat ggtaccacat gatagttccg tcgttctctt gacctgcgta
     9001 taaacgcatc aatccttgca ttccgcatgg gtttgtatat ggcaaatact ttatgttgaa
     9061 atgcacttcc aaaaacgccg gtagtccatt cccagttgtt gaaatgagcc aaatcaagaa
     9121 caacactctt tccttctttg taaaattgtt ccatcagctc aggattattg accttataac
     9181 gcttcgcaag tgcgcggcgc gacattttat agcccttgcc ggcctcgaag agcatgtcgg
     9241 caaaatagcg gtaaaattgc ctttcgatac ggttgagctc cttgtcatct ttttcgggga
     9301 aagatgcatg caggttgcgt tgtactacct tgcgccggta acggattaca tgacgcaaca
     9361 gaaaagcgtt cacattgcag agcccataca tcagccagaa aggcatgacg cggatcagcg
     9421 tctgcatgaa tatgaaaaca acatactgaa acatgatgct aaagtaccaa tttttttaaa
     9481 gaaaatatta agcatgaagc aatactttga ataatttgta tattcgtgtc ttcattttct
     9541 ctatggtttt gttttctgat taagccctct atctgtatga aacatgttta cactttgttt
     9601 tttttatgta ctgttttttt gtctcagcgg gttttttcgc aggaaatgtg gggtgtggtg
     9661 aacagtaatt atgccggtat caattccaat ttaatcaatc cggcaggtat ggtggactca
     9721 aaatactggc tcgatattaa tattgtgact cttgatgtct ttgctgaaaa caatagtctg
     9781 tatgtgccgc acaaagagct tttctttttg gaaattcttt cgccggctgt cgattttggc
     9841 gatgaaactc cagttttgaa agatacttat gataagcagc ggaacctgca tgcaaatttc
     9901 agcacccggc tcgagttgcc ttcattcatg ctttgcaagg gcgatcaggc attcggcctg
     9961 cagatgtctg tgcgtggagc cggcagtacg cgcgggattg aataccatac ttcaaaattt
    10021 atttatgagg ggattgactt cgaccctcag caaaatgaat tttatgagct gcgcaaaatg
    10081 aaaacatcgg cattgtcgtg gggcgaaata gcattgagtt attcgaaagc ttttacaaca
    10141 ttttcctcaa cttatatggc tattggtgca acgctgaaaa aaacgatggg attgggtggg
    10201 gcttatttca atgcacctta tgccaattac attgtgtatg atgactcaac actggctgta
    10261 gaagaacttg atgctgaatt tgctgcggct atgcccatgg attattcaaa caacgaatac
    10321 tcttccgacc agggcatgtt tctgggcggt ggctgggcgg ttgatctcgg ttttgttttt
    10381 cagaaaaagc gcgatggtaa tggttttatg cgggtcagac agctttgcgc gcaaaagtgg
    10441 agctcctaca aatacaaatt tggcatcagc attcttgatc tcggatacgt gaaattccgc
    10501 gacaatgcct ttgtctattc atacgacgac gcgcagacat actggcccgg atttgaccgg
    10561 tttgatccac agagtgttga cggtttcaga aacgaattca attatcattt tgcgcacaac
    10621 accgaagcag atgcaagaga attcaccatt ggcctgccga cagctgtttc gttgcaatac
    10681 gagtggcatc cggtggggta ttggttcttc aatacttcat ttgtgtatcc gatgccggtg
    10741 tttaagaatt cagttatcag gcctactcag ctcagcttca caccacgatt cgaaaagcgt
    10801 aggtttgaac tcagcattcc gttatcggtt tacgagtgga gcagacttcg tgcaggtctt
    10861 gctttacgat tctggaacct gaccatcggc accgattatt tttcatcgtg gaccggctgg
    10921 ttcgattttt acggcagcga tatttatgtc agctggaaaa tgagtttcgc caaaggatac
    10981 tgcaaaaaag gtcagtcgaa attccacaaa gggaaacgct attacaaaga cgcctgcccg
    11041 gatttctgat taatcggggc taatcacgag gctcaaacac ttctacagat agctcatcga
    11101 ccaaaatggg ctcacctgtc atattccaca caaatgttcg gaatatgtca ttttttcgcc
    11161 tgacttcggg ggtgagataa tcaaaagaaa tatcattcca ttnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nngattgagc ataccaccat gttgaccaac atgataccag
    11341 atataaatta acgcaggtgt ataccagcaa tgaccagaat attttcctgt tctttttata
    11401 tacaaatatg aatcctgtgc aggcaagaag cattaaggga gtgtaaacaa accagccttt
    11461 tctgaaacta aataaaacat tggcgataaa gggtgaaaaa aactccatac cttctcccgg
    11521 attagaccca taactgtaat acaaaaagtg acctgtgtgc aatttccagt acagtagctg
    11581 gcagaagccc agcgccagca caatgaagcc gaatacagct atctgtccaa aatgattaaa
    11641 tatagttttg aatttctcct gcaatgcttt ccatgaatca acactccata aggcaggaat
    11701 ggcaagaata acaatttctg ttgggcggct aagtattgca agtccgcatg ccagcccaag
    11761 aaagacaata aatttcactt tatatgtttc atgccatcgt attgtaaacc acaatgccat
    11821 gcaataaagc gcgaacaaat aattatgcga catggcattc tgcccgtaca tggccatgtg
    11881 aacaggaata tttgtccctg caactattat cagcaaagca atcgctgtaa ctttctcgct
    11941 ggaatatttc agcaacactt ttcttagaaa ccagaatcct gcaatggaat aaattattcc
    12001 attaagcaac aaagagaatt gatatgggaa tgaatatcca tcagcagcat atcccaatgc
    12061 aggagccaga gtatgtgcaa taaaaaagaa tggtgaatac agtatggcaa gtcccattga
    12121 gtatttcatc acatacgttc cgtctggcag caatgttgcc tgatagaaag ttgagctgct
    12181 tccgtatttt tccatcagtg acagaacctg tgctggatta tgaatgctca ggtcgttata
    12241 aatgaatgtt tgaggcagat atagataata tccgtaaata tcgtagctga taatctggtt
    12301 aggaaagaaa acaaaattac tgattatgag atatgccgca attatcgcga atgcaatcaa
    12361 tgataagctg gttttgcagg tattcacaat atttattttg agcaaatgta agtaaaatac
    12421 ttttattact tttggaaaaa aatgaggcag gttatttcga ttgtcattcc ggcctacaac
    12481 gaggcaaaaa ccatccatct gattcttga
//
LOCUS       DAOPKE010000081        12237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5445_length_12237_cov_11.796749, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000081 DAOPKE010000000
VERSION     DAOPKE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_12237_cov_11.796749"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKY63_09870"
     CDS             <1..315
                     /locus_tag="PKY63_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite S-adenosylmethyltransferase"
                     /protein_id="HPF00962.1"
                     /translation="GGHFSVSDVVLSGPLPEPILKAAELYAGCVAGASLKSDYLKMLK
                     DADFVNIRVDKEKVIELSNDLLKNYMNDAEISEFRTSGSKVLSINVYGEKRKSICCCC
                     GC"
     gene            368..730
                     /locus_tag="PKY63_09875"
     CDS             368..730
                     /locus_tag="PKY63_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPF00963.1"
                     /translation="MRNDKDNLIVSKVVDFGSQILDLSQKMEDKRQYVIARQICKSGT
                     SIGANVWEAQNAESVADFIHKLKIAAKEADETAYWFAICKRNPKYEYNDELKEDLLEI
                     SKMLSKIIASSKRRLNGQ"
     gene            797..1873
                     /gene="arsB"
                     /locus_tag="PKY63_09880"
     CDS             797..1873
                     /gene="arsB"
                     /locus_tag="PKY63_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPF00964.1"
                     /translation="MKTRLKFLDRFLTLWIFLAMAIGVLSGYFFPEVADFWESLKSSP
                     ESTTNIPLAIGLILMMYPPLAKVKYEELGDVFRNVKVLSLSLVQNWVIGPLLMFALAI
                     VFIKIFPGEDYSNLPYMYGIIMIGLARCIAMVIVWNDLAKGDTQYAAGLVAFNSIFQV
                     LFFSVYAYFFIAVMPGWFGVPTNSVVENITMGQIAESVFIYLGIPFISGFLTRFILIR
                     VKDKTWYHTKFIPVISPLTLIALLFTIMVMFSLKGEYIVKIPMDVVLIAVPLLIYFVI
                     MFVGSFWMSKRIGATYRQSVTLSFTAASNNFELAIAVAIAIFGMNSGEAFAAVIGPLV
                     EVPVMIALVNLAFWYRKKWFGIQA"
     gene            complement(2040..3932)
                     /locus_tag="PKY63_09885"
     CDS             complement(2040..3932)
                     /locus_tag="PKY63_09885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPF00965.1"
                     /translation="MKTLTAFCITLLLIHFSFNSLAQHPTISTNESLAYEKGTRNHDG
                     TPGENYWQNSSDYFIQVTVDVNNKVISGRERIVYSNNSPDSLKTIVIRLYQDVFKKGI
                     NRNSIVPVHPADIHDGVRITSLKVNNDIVDVSENSRQLMRDGTLMYLILSKKMAPHSK
                     TELQIEWNFDFPQHTLIRMGTIDSTSMFVGQWYPQIAVYDDINRWDTRSYNGMAEFYS
                     DFSNFEVEITVPDQFVVWATGDPQNLKDVLQAPIYSRYTEACTSNEIHHVITEEDILN
                     KKITTQNLTWKYKATNVSDFAFGISDHYLWDVTSVVVDKSSGRRTVVETAYNKSAVNF
                     DSVISISRETIRSLSEEMPGIPYPFPYLTVYAGDFGMEYPMITNVGPDEDFGMTVYAN
                     SHEITHAYFPFLVGTNETVCGWMDEGLTVFIPENSQHEIATELNIGAYNTSAFAHYAG
                     IQDEVALITPTHYLDPGIYFYLNYAKGEQALRMLEMELGPELFKTCLLTFIDRWKYKH
                     PTALDFFNTFSNVSKRDLNWYWMAWYYQTGGIPDLAIKSLTKSGNKYTVVVENKGQLP
                     LPISLTFYSYKKAVGTISASASKWQGGQNETVLVFESKEPFTKIVLGSETIPDANPDD
                     NVYLVK"
     gene            4392..5012
                     /locus_tag="PKY63_09890"
     CDS             4392..5012
                     /locus_tag="PKY63_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00966.1"
                     /translation="MHFIDEMNARQSALYDQINAFNKENDAVGEQIDPSKMTAAYGMT
                     QDVNALMERQQRSLDNQEMNEKLAQRTMEYESQWKKLVDSLSAEMAVFNVLYTEWADN
                     CLGIPSSNCNACEAKKNQKGDEILMRYFFGSNAILIGWLNTYVGDVPVHSKQVMIEAM
                     SIQEETLGFKFPHSADLAALEEQKAIIDAILKVFEEIDLKLFPGAN"
     gene            5189..7612
                     /locus_tag="PKY63_09895"
     CDS             5189..7612
                     /locus_tag="PKY63_09895"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HPF00967.1"
                     /translation="MKFLLCILSFLIVSGIHTAVSATGIVVTGVLQDSTDQKPLAFAN
                     VALIHQSDSSFLTGTTTDENGQFELNVTDTGQYFLRVSYLGYGTQFKQLDLKNGQSRL
                     DLGRIPVAKSAQNLSEVTIVAEKPMYVYEGEKKVYNVSEDPSVQGGVANDALQNAPGV
                     YVDMEGNITLRGVSGVEIWINDKPSRISAEGLKQFLQQLPANSLERIEVITNPSAKYS
                     AEGTGGIINIVTREKIKKNLLLSFGLNASTLGNYSPWASFVASNEKLSFNIYLSHSNH
                     NWKGNNRSSGFVFNGPDTVYSFSNGSDYTYEGNWNYGHMSLSYDFNKNNSIETWFGGS
                     FSSNESSSSSYSLRETSGESFNYTSQNAGNRTGNSMNGGLSYEHRFKKEGHNFKLDSY
                     FWTWDQDGETSYSKLFDVQTWDNLKYIENGINSNLSFSGEAHYTNPLAKNRTIEAGGE
                     FGSNVQSEKSPIDTFNFSTGLYEHVPAFSNVLDQYTMAGAFYTTYADTLKWLNYKVGL
                     RYEFAGLEMTSVALTDVLKRNYGTFFPTVHLSAKTKNNDNYTLSYSRRVQYPQWELDP
                     FANRINEESVFFGNPYLDPAFTDAFEAGYAHFFKNGSSISATLYHRRTNLDITTKSEG
                     VFDTLLNRYTIYTSYINAGKNISTGGDFTATWRPKPAYRIMFNTNVYYRDFYADLGSY
                     VVDKEDLTYDGKIIFMWNYKFLRLNIMGIYRAAAASLQGSAEDTYFVNANLNADFFKR
                     KLSVRVGMQDIFNWQESNTITNTPTYYSENYSKNRSQFLTFGITFRFGKIELEQSQMP
                     PQGGVGGQM"
     gene            complement(7632..8762)
                     /locus_tag="PKY63_09900"
     CDS             complement(7632..8762)
                     /locus_tag="PKY63_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF00968.1"
                     /translation="MEAPFVFGKTARLDEFTDRETQTERLITNFNSGTNTILISPRRW
                     GKTSLVLHTARVYEKRYKSNRVVIIDLNNIRSEQEFYEKYVNQIMRAVSTKITEFTEN
                     AKTFLSRFIPRISYSPDRQNEISVGLDWEAAGQDPDEIINLPQKIAAKKGIRIIVCLD
                     EFQNIMVFDNPTAFQKKLRSHWQNHSRVTYCLYGSRRGLMTELFAHSSMPFYKFGDLM
                     FLEKIEEKLWVKFFIKRFKDTGKKINATDAALIAQKVENHPFYAQQLAQLTWLRTKKT
                     TNTQIVIQAAESLLDQLSYLFQNLADSLPSNQLCFLQAIINNETKLTSGAVISRYKLK
                     SSAHVAKIKKALIDKDLIDYHNRQYNLNDPMFKLWLKTRYFV"
     gene            9050..9442
                     /locus_tag="PKY63_09905"
     CDS             9050..9442
                     /locus_tag="PKY63_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF00969.1"
                     /translation="MLSFSLIPVNQTFILEGVRHVLPINAREAFESNLALAVDVREAM
                     EVMVSSIDCPQLLLYPIGQLNEMCAELPLNTPIIVICNHGIRSAEAVVLLQQKGFTEV
                     YNLDGGLHGWIEAGLPVAGQGGCSCGCH"
     assembly_gap    9832..9931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10011..12077
                     /locus_tag="PKY63_09910"
     CDS             10011..12077
                     /locus_tag="PKY63_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00970.1"
                     /translation="MKKVYLIAVMTLAGAFLFSACSPKNDKEKKSMSSLKKETVTTAK
                     DSLMAKFGKTDEIRITKGVDQAAEMWRKEDGADSTFIRFCIDNFTATGPDLDTLFDKY
                     NYYFEQIYGHYNMMTIELMRNIHEPRGTVMPVDEMFGSWDVTAHFAEDMFNNKTAFAV
                     LLNFPYYTLEEKNDLGKKWTARQWGMARLGDMFIARAPSDLLLKASETMVNGDSYISN
                     YNIYMGSLLNDKGEKYFSEDKVLITHWGLRDELKSNYADTSLGLAKQEMIYQVMQRII
                     DQTIPQKVINNGSVMWNPVTNKVTGNGKEVDSEPEPDKRYEVLLNNFKALKALDSYFP
                     YNENYISRKFDAEMEMAQADVENLFVSYVSSPVLKEIGLLIEQRLGRKLRPFDIWYNG
                     FTSRAGLNEAELDKTTQKLYPDNVAFKKALPGILKKLGFDAEKAEYISTYIDVDPAVG
                     SGHAWGSAMRNDVSHLRTRIAPTGMNYKGYNIAVHEFGHNVEQTISIHFVDNYMLHGV
                     PNTAFTEAIAFVFQSRDLQLLDMKNNDPLAHEYENLEIAWSVYEIMGVSLVDMNVWKW
                     MYEHPDATPKELKEQVIIIAKDIWNKYYAPVFGIKDQTILAVYSHMIDAPLYLSAYPL
                     GHLIQFQLEQHLKGKNFAAEVTRIYSLGRLTPDVWMVQATGSKLSTNPLLKAAAESVK
                     VVKATK"
BASE COUNT         3348 a         2421 c         2647 g         3721 t
ORIGIN      
        1 ggcggacatt tcagcgtgtc ggatgttgtg ctctccgggc cgctgccgga gccgattctg
       61 aaagctgctg aattatatgc tggttgcgtt gccggagcaa gtctgaaatc agattatctg
      121 aaaatgctca aagatgctga ctttgttaat atcagagtcg ataaggaaaa agtgattgaa
      181 ctgagcaatg atcttttgaa aaattacatg aacgacgctg agatttctga atttcgtact
      241 tcgggatcaa aggttttaag catcaatgtc tatggtgaaa aacgtaaaag catttgctgt
      301 tgctgcggtt gttaatttga aaattcgatg atttgaaaat ttgaaaatta agctaaacac
      361 tataattatg agaaacgaca aggataattt gatagtcagc aaagttgttg attttggatc
      421 tcagattctg gatctgtcgc aaaaaatgga agacaagcgt caatacgtaa tcgcgcgcca
      481 gatatgcaag tctggcacct caattggcgc caacgtttgg gaagcacaga atgctgagag
      541 tgtggctgat tttattcaca aattgaaaat agctgcaaag gaagctgatg aaaccgccta
      601 ctggtttgca atctgtaaaa gaaatcccaa gtatgagtat aacgacgagt tgaaagaaga
      661 tttacttgaa atcagtaaaa tgctttcaaa aattattgct tcatccaaac gcaggctaaa
      721 tggacaatag aaattttcaa atcaaggaaa gatctttcaa attagcacac tttcaaattt
      781 tcaaattctt gcacgcatga aaacaagact aaaatttctc gacagatttc tcacattatg
      841 gatctttctc gcaatggcca ttggtgtcct atccggatat ttctttcctg aagttgctga
      901 tttctgggaa tcattgaaat cgtcgccgga aagcaccact aatatcccgc tggcaatagg
      961 ccttattctc atgatgtatc caccgctggc aaaagtaaaa tacgaagagc tcggcgatgt
     1021 gttccgcaat gtgaaagtgt tatcgctttc tcttgttcag aactgggtca tcgggccctt
     1081 gcttatgttt gcgctcgcta ttgtttttat taaaattttt ccaggcgaag actattcgaa
     1141 cctgccttat atgtacggta tcattatgat tggtctcgca cgttgtatcg ccatggtcat
     1201 tgtttggaac gatcttgcaa agggcgatac gcaatatgcc gccggacttg ttgcattcaa
     1261 cagtattttt caggtgttgt ttttttctgt ttatgcctat ttctttatcg ctgtcatgcc
     1321 gggctggttc ggtgttccaa ccaacagcgt ggttgaaaat atcaccatgg gacaaattgc
     1381 cgaaagcgta ttcatctatc tcggcatacc ctttatatcc ggcttcctga cccgttttat
     1441 actgatacgc gtcaaagaca aaacctggta tcatacgaaa tttattccgg tgattagccc
     1501 gctgactctc attgcgctgc tgtttacaat catggtcatg ttctcactta aaggcgaata
     1561 cattgtaaaa attccgatgg atgtggtttt gattgcagtg ccactgctga tttattttgt
     1621 gattatgttt gtgggttcat tctggatgag taaaaggatt ggtgcgactt acagacaatc
     1681 ggtcacattg tcttttacgg cagccagcaa caatttcgag cttgcgattg ccgttgccat
     1741 tgctattttc gggatgaatt caggcgaagc ttttgcagct gtcatcggcc cgctggttga
     1801 agttccggtc atgattgcac tggtgaatct ggcgttctgg tatcggaaaa aatggttcgg
     1861 catacaggct taaagttttg cgttgagccc ttcccgacat ttttgcgtca gctttagtca
     1921 ctctgagcgg agtcgaagag ttgacaacag acatgttgtc tgttatttta tctgaccagc
     1981 aaagaagacg tcgggatttg cattgagctt tgagcgttgt gctctgcgcg ataagcgaat
     2041 catttcacca gatacacatt atcatccgga ttggcatcag gaatggtttc gctgccaaga
     2101 acaatttttg taaacggttc ctttgattca aaaaccagca cggtttcatt ctgaccacct
     2161 tgccatttgc ttgctgacgc agaaatggtt cccacagctt ttttatatga ataaaaggtc
     2221 agtgatattg gaagcggcaa ttggcctttg ttttcaacta ctactgtata tttgtttcct
     2281 gattttgtca aactttttat tgcaagatcc ggaatcccgc cggtctgata ataccaagcc
     2341 atccagtacc agttcaggtc gcgctttgat acattgctga atgtattgaa gaaatcaagc
     2401 gctgtcgggt gtttgtattt ccagcggtcg atgaaagtta gcagacatgt tttgaaaagt
     2461 tcaggcccaa gctccatttc aagcattcgt aatgcttgtt ctccttttgc gtagttcaga
     2521 taaaaataaa tgcccggatc aagataatgc gttggcgtaa taagtgccac ttcgtcctga
     2581 atgccggcat agtgcgcaaa ggcgctggta ttgtatgctc cgatgttaag ttccgtcgca
     2641 atttcgtgct gactgttttc aggaatgaac acagtcagtc cttcatccat ccagccacaa
     2701 actgtttcgt tggtgccaac cagaaacggg aaatacgcgt gcgtaatttc atgcgaattg
     2761 gcatacacag tcatcccgaa atcctcatca ggaccaacat tggtgatcat cggatattcc
     2821 attccaaagt cgccggcata caccgtcaga tatggaaaag ggtagggaat gcctggcatt
     2881 tcttctgaca acgaccggat tgtttcgcgc gatatcgaaa ttacactgtc aaaattcact
     2941 gccgatttat tgtaggcagt ttccaccacc gtgcgtctgc cactgctttt gtctactaca
     3001 acgcttgtaa catcccacag ataatgatcg ctgattccaa aagcaaaatc actcacattc
     3061 gtcgccttgt atttccatgt gagattttgc gttgtgattt ttttattcag aatatcttct
     3121 tctgtaatta catgatgaat ttcgttggat gtacatgctt ctgtatatcg gctgtaaatg
     3181 ggcgcttgaa gcacgtcttt cagattctgt ggatcacctg tagcccacac cacaaactga
     3241 tcgggaacgg ttatttcgac ttcgaaattg ctgaaatcgc tgtagaattc tgccattccg
     3301 ttatatgacc tcgtgtccca gcgattgatg tcgtcgtaca ccgcaatctg cggataccac
     3361 tggccgacaa acatgcttgt gctgtcaata gtacccatgc ggattagcgt gtgttgcgga
     3421 aaatcaaaat tccattcgat ctgaagttct gttttgctat gcggcgccat tttttttgaa
     3481 agaatcaaat acatcagcgt tccatcgcgc atcagctgcc ggctgttttc gcttacatca
     3541 actatatcgt tgttcacttt cagcgatgtg atgcgcacgc cgtcgtgtat atcggccgga
     3601 tgtacaggaa ctatcgaatt gcggtttatg ccttttttaa atacatcctg atataatctg
     3661 atgacaattg ttttcaatga atcgggactg ttgtttgaat atacaatgcg ttcgcggccg
     3721 cttattacct tgttattcac atctactgtt acttgtataa aatagtctga cgaattttgc
     3781 cagtagtttt caccaggtgt tccatcgtgg ttccgggtgc ctttttcgta tgccagtgat
     3841 tcgtttgttg aaatagtggg atgctgcgcc agtgaattaa acgagaagtg tatcagtagc
     3901 agggtgatgc aaaaagcagt aagggttttc atgtgatgtg gattttttta attaaatcag
     3961 ccaaaagtaa aaattcatca attgacacac atatattttc taccattacc cctttttatc
     4021 ggataaacgc cgcgaacatt tctttgttgt tctttcttgt ttgctgccat gctgacgagg
     4081 atatatccga aagctgccag ggaaagtgtc tggagaaagg actctgcctg catgtcgctg
     4141 caatcaactg aatttatgat ttgcaggatt attcaataaa ttattttcga ctcttgaaat
     4201 gagttttttt taatgcgttg gctctgaatt tttccggcat gatttttatt gtgcgcgtga
     4261 aattttttat ctttgaaaaa aaaccaccta tgaaaacaaa tctttttctt gtcgtcgtat
     4321 ttttgataat tgctccgtcg gtagttttcg ggcagattcc tgcatttcct gaaaaatcga
     4381 gagaggccgt catgcatttt attgatgaaa tgaacgcccg gcaatctgct ttgtatgatc
     4441 agattaatgc ctttaataaa gaaaatgatg cggtcggaga gcaaatcgat ccttcgaaaa
     4501 tgactgcagc atatggtatg actcaagatg taaatgcgct gatggaacgg cagcaacgtt
     4561 cgctggataa tcaggaaatg aatgaaaagc ttgcgcaaag aactatggag tacgaatcac
     4621 agtggaaaaa actggttgat agtttgtcgg cagaaatggc agtttttaat gtcctttata
     4681 cggaatgggc tgacaactgt ttgggcattc cttcttccaa ctgtaatgct tgtgaagcaa
     4741 aaaagaatca aaaaggagat gaaattctta tgcgctattt ttttggtagc aatgcaatac
     4801 ttatcggctg gctaaatact tatgtggggg atgtgcctgt gcattcgaag caagtgatga
     4861 ttgaagcaat gagtattcag gaagaaacac tgggtttcaa gttccctcac tcagccgatc
     4921 tggccgcact cgaagagcag aaagccatta ttgatgcaat tcttaaagtt tttgaagaaa
     4981 tagatctgaa gctctttccg ggcgccaatt gataattgtt ccaccggcgc gcgtgcccac
     5041 acattttttt agccttttga taaagcagtc catgctttct accaaagcca ctccggactg
     5101 tttggtagat aaaatattcg tataagtata gaatattata caataattta caattttgta
     5161 agtttgtatg atattaaact ccactgaaat gaaatttctg ctttgcatat taagtttttt
     5221 aattgtttca ggtatccata cagcagtatc tgctaccggt attgttgtta cgggcgtgtt
     5281 gcaagacagt accgatcaga agccgcttgc gtttgccaat gtggccttga ttcatcaaag
     5341 tgattcgtct tttcttacgg gaaccacgac tgatgaaaat ggtcagtttg aattgaatgt
     5401 cactgataca ggtcagtatt ttcttagggt atcttacctt ggttatggca ctcagtttaa
     5461 gcagcttgat ctgaaaaacg gtcagtcgcg gctggatctt ggccgaattc cggttgccaa
     5521 atcggctcag aatctttcag aagtaacaat tgttgctgaa aaacctatgt atgtttacga
     5581 aggcgaaaag aaagtgtata atgtgagtga agacccgagt gtgcagggtg gagtggccaa
     5641 tgatgccctg cagaatgccc cgggcgtgta tgttgatatg gaaggaaata tcactttgcg
     5701 tggagtttcg ggtgtcgaaa tttggattaa tgataaaccg tcacgtattt ctgcagaagg
     5761 attgaagcag tttctgcaac agttgcctgc caattctctc gaacgcatag aggttattac
     5821 aaatccatcg gccaaataca gtgccgaagg gactggtgga atcattaata ttgttacccg
     5881 cgaaaaaatt aaaaaaaatc tcttgcttag ttttggactt aatgcttcaa ctcttggtaa
     5941 ttattctccc tgggcatcgt ttgttgccag caatgaaaaa ctgagcttca atatttatct
     6001 gagccattcc aatcataact ggaaagggaa taatcgttct tctggttttg tttttaatgg
     6061 tcccgacacc gtttattctt ttagcaatgg ttccgattac acgtatgagg gcaactggaa
     6121 ttacggacat atgagtcttt cctacgattt taacaaaaac aattcgatcg aaacctggtt
     6181 tggcggctca ttcagcagca atgaaagctc atcatccagt tatagtttgc gtgaaacatc
     6241 aggcgaatct ttcaattaca cttcccaaaa cgctggcaat cgcaccggta acagtatgaa
     6301 tggcggtctt tcatacgaac atcgctttaa aaaggaaggc cataatttta aactcgattc
     6361 ctatttctgg acatgggatc aggatggcga aacttcttat tcaaaactct tcgacgtgca
     6421 gacatgggac aatctcaaat acatcgaaaa cgggatcaac tccaatttgt ctttttccgg
     6481 cgaagcacac tatactaatc cgcttgcaaa aaacagaacc atagaagcgg ggggcgaatt
     6541 cggcagcaat gtgcaaagcg aaaaatcacc cattgatacc ttcaattttt ctacaggatt
     6601 gtatgaacat gttcctgctt tttcaaatgt gcttgatcaa tacaccatgg ccggtgcttt
     6661 ttatacaacg tatgccgaca ctttgaaatg gctgaattat aaagttggac tgcgatatga
     6721 atttgcaggt cttgaaatga cttcggttgc tttaaccgat gtgttgaagc gcaattacgg
     6781 aacttttttc ccaacagtgc atttgagtgc caaaacaaaa aacaacgaca attatactct
     6841 cagctattcg cggcgtgttc aatatccgca gtgggagctc gatccatttg ccaatcgtat
     6901 taatgaagaa tcggtatttt ttggcaatcc ttacctcgat ccggctttca ccgacgcatt
     6961 cgaagcgggt tacgcccact ttttcaaaaa cggaagttca atcagcgcca ctttatatca
     7021 ccgcaggacg aaccttgata ttaccactaa atcggaagga gtgtttgata ctttgctcaa
     7081 ccgttatacc atttatacat cttatatcaa cgccgggaaa aacattagta ccgggggaga
     7141 ttttacagca acttggcgcc cgaaacccgc atacagaatc atgtttaata ccaatgtcta
     7201 ttatagggat ttttatgcag atcttggatc ctatgttgtt gacaaggaag atctgaccta
     7261 cgatggtaaa attattttca tgtggaatta taaattcctg cgactgaata ttatgggcat
     7321 ctatcgcgct gctgcagcca gtttgcaggg gtcagcggaa gatacgtact tcgtaaatgc
     7381 caatcttaat gccgatttct ttaagaggaa attgtcggtt cgcgttggta tgcaggatat
     7441 tttcaactgg caggaaagca acaccattac caacactccc acttattatt ctgaaaatta
     7501 ttcaaagaac cgaagtcagt ttctgacatt cggaattact ttcaggtttg gtaaaattga
     7561 actggaacaa agtcagatgc cgcctcaggg tggtgttggt ggccagatgt aaaaaatctt
     7621 tcttaaatca atcagacaaa gtatcttgtt ttgagccaaa gtttaaacat gggatcgttg
     7681 agattgtatt gtctgttgtg gtaatcaatc aggtctttgt caatcagtgc ttttttgatt
     7741 ttcgcaacat gggccgacga ttttagtttg tagcggctaa tgacagcacc cgacgttaat
     7801 tttgtttcgt tattgatgat ggcctgaaga aagcagagtt ggtttgaagg cagactgtcg
     7861 gccagatttt gaaacaggta actgagctga tccagcagac tctcggctgc ctgaataacg
     7921 atttgagtat tggtggtttt ttttgtcctc agccaggtca gctgtgccag ttgctgcgca
     7981 taaaacggat gattctctac cttttgtgct atcagcgccg catcagttgc attgattttt
     8041 ttaccggtgt ctttgaatct tttgatgaaa aatttcaccc atagtttctc ttcaattttt
     8101 tcgagaaaca tcaggtcgcc gaatttgtag aagggcatgg aagaatgtgc gaaaagctct
     8161 gtcatcaacc cgcggcgact cccatagaga caataggtca ctcggctgtg attttgccag
     8221 tgcgagcgca gttttttctg aaaagcagtc ggattgtcaa acaccatgat gttctgaaat
     8281 tcatcgagac agacaatgat gcggatgcct ttctttgcgg caatcttctg aggcaggttg
     8341 atgatttcgt ccggatcctg accggctgct tcccaatcga gaccgaccga tatttcgttt
     8401 tgccggtccg ggctgtagct gattcgtggg atgaagcggc tcagaaatgt ttttgcattt
     8461 tcggtgaact cagtgatttt tgttgatact gcccgcataa tctgattgac atatttctca
     8521 taaaattcct gctccgaccg gatgttattc agatcaatta tcactacacg attgcttttg
     8581 tagcgttttt catacactct tgctgtatgc agaaccagtg aggttttccc ccatcgcctg
     8641 ggcgaaatca gaatggtgtt ggtgcctgaa ttgaaattgg ttatcagcct ttcagtctga
     8701 gtttcacggt ccgtaaattc atctaaacgt gccgtttttc cgaaaacaaa tggtgcttcc
     8761 atgaaggatt gattaatttg ttttaatgaa atcatcattc acttgcaaaa gtacaataat
     8821 tatttgtagt aacaaataag ttagtaacaa ataagttaga aacaaataag ttagaaacaa
     8881 ataagttaga aacaaataag ttagtaacaa ataagttagt aataaaatag ttagtaacaa
     8941 aaggttataa ttgtttatgc gatgaaaaat ttgattaatg acctttgtgt caatacaaac
     9001 agagcatccg ctcagtaaat ccactgtatt ttgtaatttt acaacaaaaa tgctgtcctt
     9061 ttctttaata ccagtcaatc aaacctttat tcttgaagga gtccggcatg ttttgccgat
     9121 taatgcccgt gaagcttttg agtcaaacct tgcgctggca gtcgatgtcc gtgaggccat
     9181 ggaagtaatg gtttcctcta tcgattgtcc tcagctgctt ttgtatccga tcggtcagct
     9241 gaatgaaatg tgcgcagagc tgccattgaa tactccgata attgttattt gcaatcacgg
     9301 aatccgcagc gcagaggcag ttgttttatt gcagcaaaaa ggattcaccg aagtgtacaa
     9361 cctcgacggc ggtctgcatg gctggattga agccggactg ccggtggcgg gccagggcgg
     9421 ctgcagttgt gggtgtcatt gatttttgcc gttacacgtt acaatcgtta catgtagctt
     9481 tcagccgtca gtaaccagct ttcagctctg acaaggagct cataactcat cacctacttc
     9541 ctcgtccccg gagccctaaa gggcatacgg gctattaata tttatcgcct cccggcgaat
     9601 cataatggaa cttcgtagct ttctctgacg ccttcagcaa ctcatcactc ataactactt
     9661 acgcctatct ccttacgcct ctctcgtatt tttgatttcg tatttcgtct ctcgtatttt
     9721 cacatggacg aagtcccaca tttccacatc atcgcatcat cgcatcatcg ctgcagtatt
     9781 tcgtatctcg tatttctaat ttctaatttc gtatttctga ttttgtattt cnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natttcgtat ttctgatttt gtatttcgta
     9961 ttctctcatt tccacattat atattatatt tgctcaccaa acacacactc atgaaaaagg
    10021 tatatttaat tgctgtgatg acccttgccg gagcattttt gttttcggcc tgcagtccta
    10081 aaaatgataa agaaaagaaa tcgatgagca gtctgaaaaa agaaacagtg actaccgcca
    10141 aagacagtct gatggcgaaa ttcggaaaga ccgacgaaat caggattacc aagggtgtcg
    10201 atcaggcggc tgaaatgtgg cgtaaggaag acggtgccga cagcactttc attcgtttct
    10261 gcattgataa tttcacagcc accggaccgg atcttgacac actgtttgac aaatataatt
    10321 attattttga acagatttat gggcactaca atatgatgac cattgagctg atgcgtaata
    10381 tacatgagcc acgcggtaca gtgatgcctg tcgatgaaat gtttggctcg tgggatgtga
    10441 ctgctcattt tgccgaggat atgtttaata acaaaacagc ttttgccgtg ttgctgaatt
    10501 ttccgtatta caccttggag gaaaagaatg atcttggtaa gaaatggaca gcccgtcagt
    10561 ggggaatggc acggcttggc gatatgttca ttgcacgtgc tccgtctgat ctgttgctga
    10621 aggcctctga aacgatggtg aacggagaca gctatatatc aaattataat atctacatgg
    10681 gaagtttgct gaatgataaa ggcgaaaaat atttctctga agataaagtg ttgattactc
    10741 actggggctt gcgcgatgag cttaaatcga attacgcaga tacgtcttta ggccttgcaa
    10801 agcaggaaat gatctaccag gtgatgcagc ggattatcga ccagactatt cctcagaagg
    10861 tcattaacaa cggttctgtt atgtggaacc ctgttacaaa taaagtgacc ggcaatggca
    10921 aagaagtgga ttcagaacct gaaccggaca agcgttacga agtattgttg aataatttca
    10981 aggcattgaa agctctggat tcctattttc cgtacaatga aaattatatt tcgcgaaagt
    11041 tcgatgcaga gatggaaatg gctcaggccg atgttgaaaa tttgtttgtt tcctatgtgt
    11101 cttctcccgt gctgaaagaa atcggattgt tgattgaaca acgtttgggt cgaaaattgc
    11161 gtccgttcga tatttggtac aatggcttta catcacgcgc aggccttaat gaggctgagc
    11221 ttgacaaaac cacacagaaa ctgtatcccg ataatgtggc tttcaaaaaa gcactgccgg
    11281 gtattctgaa aaaacttggt ttcgatgcag aaaaagcgga atacatttcc acttacattg
    11341 atgttgaccc ggctgtaggc agtggccacg cctggggttc ggccatgcgt aatgatgtta
    11401 gccatcttcg tacccgcatt gctccaacag gaatgaatta caaaggatac aacattgcag
    11461 tgcatgaatt tggtcacaat gttgagcaaa ccatttcgat tcatttcgtt gacaattaca
    11521 tgctgcatgg tgttcccaat accgctttca cagaggcgat tgcttttgtt ttccagagcc
    11581 gtgacctgca attacttgat atgaaaaaca atgatccgct ggcgcatgaa tatgaaaatc
    11641 ttgagattgc atggtcggtg tatgaaatta tgggcgtcag tctggtggat atgaatgtat
    11701 ggaaatggat gtatgaacac cccgatgcaa ccccaaaaga attgaaggag caggtgatta
    11761 ttattgctaa ggacatttgg aacaaatatt atgcaccggt gtttggaata aaggatcaaa
    11821 ccattctggc tgtttatagc cacatgattg atgcgccgct ctatttatcg gcttatccac
    11881 tgggccacct gattcagttt cagcttgaac aacacctgaa aggaaagaat tttgcggctg
    11941 aagtgacacg tatttattca ttgggtcgtc tcactcccga tgtatggatg gttcaggcaa
    12001 ccggaagtaa attgtcaaca aatccattgc tgaaagctgc ggcggaatca gttaaagttg
    12061 tgaaagctac gaaatagcaa tttcatataa atcgatattt tttgcctccg gaattttatt
    12121 ttccggaggt tttttgtgaa aaaatattga attttcgaaa ataattttac cctgcgcttt
    12181 ttttactgtc aaagaaaggt tttaagaaaa ttctgtaccg tgaaagtgaa agcgatt
//
LOCUS       DAOPKE010000082        11948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5614_length_11948_cov_12.557639, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000082 DAOPKE010000000
VERSION     DAOPKE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_11948_cov_12.557639"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..600
                     /locus_tag="PKY63_09915"
     CDS             103..600
                     /locus_tag="PKY63_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPF00971.1"
                     /translation="MKRILFSFCLIAGLMTALYAQLYTGGYKSFNDSAFSKGDKILAP
                     KILFTLSGGSRVLPECEDSVKIIADFLNEHPEMCIEIGVHTDYRGADEFNDTISYHRA
                     GSVKEVLVGLFEIDPTRIEIKGYGKQQPLISKEEIDAVQSKEMQETMHRINRRVEVKI
                     IQIQQ"
     gene            609..1163
                     /locus_tag="PKY63_09920"
     CDS             609..1163
                     /locus_tag="PKY63_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00972.1"
                     /translation="MRIDINQKKISIGDKYQIFINGQQCYSGAKKLFRFLAKIELFDQ
                     LNQQVKLTIKKKLSLFRAKFRIIFPIGTDIQFETVSYWKLHYRCVLNKDTYEIYGHRS
                     RKYSVYKNGIQIAWWDKEVVSWFNGDNYTIVADNDCNYELIIAFCLIIDEFRSDDNKS
                     AVNVDIGHIGPQATPFNPNWQPKN"
     gene            complement(1451..2020)
                     /locus_tag="PKY63_09925"
     CDS             complement(1451..2020)
                     /locus_tag="PKY63_09925"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPF00973.1"
                     /translation="MIQEKHIWKPGNMLYPLPVVMVSCGNSEKHNIITIAWTGTLCTT
                     PPMVYISVRPERYSYAMIKEQMAFTINLVDKNLVKAADFCGVRSGKDLDKFKTMKLTP
                     VAGIQIPCPYIAESPLSMECKVTQIIELGSHHMFVAEVLNTIADERFFNTRTSKFEIE
                     RAALINYNHGGYFEHGDLVGTFGYSVKKK"
     gene            2683..3762
                     /locus_tag="PKY63_09930"
     CDS             2683..3762
                     /locus_tag="PKY63_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF00974.1"
                     /translation="MGQNAPRFKLEKLPPLREKVETIEILRQTNKSTAVLAELKGVAK
                     TIPNQAMLINAIVLQEAKDSSEIENIITTQDELYQALAINKPHISTEIKEVVNYRKAI
                     FHGFDLLKKQEFLRVNDIISIQQKLVDNTAGIRSTPGTVLKNDKTGDVVYTPPQDKAE
                     ILDLLSNFIEHFNHSGDLSPLINLAVFHYQFESIHPFYDGNGRTGRILNILYLILNKL
                     IDVPILYLSSYIIDHKPEYYRLLNQTNSSGNWQEWILFMLKAVESTSKDTITKILNIK
                     NQLDSTILKVQEKAPKIYRKELVELLFEQPYSKIEFVVHKLGVERKAASRYLRELEMI
                     GIVESKKIGRETIYVNRDLIEILKK"
     gene            4152..4406
                     /locus_tag="PKY63_09935"
     CDS             4152..4406
                     /locus_tag="PKY63_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00975.1"
                     /translation="MHTIELEKLCDPVRIFIGFSVDTAGKISNADFIPCIFENDDCSI
                     DSAYLEKLQVEFETSMPVWKPEINNDSITKTRYIIPVIFD"
     gene            complement(4477..>6501)
                     /locus_tag="PKY63_09940"
     CDS             complement(4477..>6501)
                     /locus_tag="PKY63_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00976.1"
                     /translation="VADNNNQNVQVSNASDKVSASDEEQDLIQSSFYESAAQTFMVRI
                     ASLKKKLATNPADSNQIKNEIAALTKTMQAYSAEADAYKNNAENNLKLIGKQLPEQIP
                     VYSEESVIGFHQLMYETELHKADSVRRLADATTDPAVKTKFQKQADEYTESSKRHFLM
                     ANDLYGIWNNSEYENNNVALTESNAARSQESDNARMLMIEARQLRSQAYRENDFAKQR
                     DILAQAQEKEQQSLDLQQKAMAANNIDDPEKIVSRTVSEIENTTGNDIAEITKQYPSN
                     TALLAAYEKSAVTNQDVADNNQNNNQVNNQNINQNNNQNNNQNNNVVNNNVVVDNNQL
                     SVVSSQLPADSTDRSVGDDSSTIKSDNRLYYRIQISASRVEVDTTKYFKGMNVVVDKT
                     GGWNQYMTGYFTSYNIANTDLKRVRQQGYADAFVVAYFDGKRVPVYEARRMEQGGTPA
                     VTAQAASAGNFSMTDVEGLVYSVQVGVYATTRNSTSLFGISPLIEERMDNKYYRYYAG
                     TFNNMNDAVAARNKIRAGGVPDAFVVILYKGKKITQAEANQLMADGVTFGGGLKQSGA
                     GTQPQNTQVTPTNITPASKPVFKVQIGAYSKDVPVNVVNELIALAGQGIETTRNDAGL
                     TVYTVGNFSKYSDAEAKKNELNAKGLTDAFVVAYVDGKRVSVSEARKVAE"
     assembly_gap    6502..6601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6602..9480)
                     /locus_tag="PKY63_09945"
     CDS             complement(<6602..9480)
                     /locus_tag="PKY63_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00977.1"
                     /translation="MPTVKNIFFIYLISCIFMLSGFEALSQPVGDDLQVKTEADRLFV
                     EQKYGEAFPLYSQLLSIHRKDPYYSYRFGVCLLYTDRRDPEAPLKYMEPFVGQLSGED
                     SYMIYFHLGVAYHQAYRFGEALRAFEAFKGKVPSKSPYAKEAFIRNQMCSNGITLLQN
                     LSDLVVMKKYEVGTESFFRSYDISKFGGRIGPKPEFLKTKPDRRSADPELVFISDTHQ
                     VVYYSSYGKKNMNGRDIYRVYKLPGGKFSAPEKLSSAVNTEYDENYPYLLPDGRTLYF
                     CSKGHNSMGGYDIFRTVYDTVKKDWTAPVNVDFAINSPFDDILFITDPSQRYAWFSSL
                     RNSSDGRMMVFLVRIDRKIPDYENYNPELIAAGNIDVNSAEYLETIAKLREKASLDVN
                     SNEEIVIDTAKVIAVNNNSKYNIPENPTGDQVVDIAFEHVASAEDQLDVFRNMRDASL
                     KISEQKKDQAAALSKQSSDLYEKSVNETDAKKRKDMMAQSNELSKKSMLLSEESELAK
                     QMYNQYAAAADVQAKNFDRIQDKAGNVQKLALSRQIDTSVVLLKDLIDGIDTFKINLV
                     QLDKKIRGDNEYVAELEKVVASDLAESKILEEDAKKLDKDAEVYINEAALTTDPELKE
                     EYQSEADKIRREANDKRLKAQQLKDDAKLKQNEMVDLKSKSDQRVDIVSDAVAYADSL
                     NSDSDNNNQQSDVSNQNVTDNNNQNIADNNNQNNTDVNNQNIADNNNQQATDTNQVIT
                     DNNQQSTELVTSIIQQISSSADSVQNCLDRNKRESEAVSLAFAKKKSDYDFKLSEWVK
                     LRDKPVLTQSEQMKKTELEADLTEINMQMNVLSDYHDELNKQNALLTQAESDFDKLMQ
                     ELSDKAAADTNELKKMQTRSEAILETSRPAADDTEKLLSTRTGDFRSEISKTEKERNE
                     LQPQLNIVEKKLQVLNEKLENTDNPKKRTQIENDIADTKEKQNALT"
     gene            9653..10537
                     /locus_tag="PKY63_09950"
     CDS             9653..10537
                     /locus_tag="PKY63_09950"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPF00978.1"
                     /translation="MKITIDSKSGFCAGVTSAIHGAEEELKVNQKLYCLGDLVHNSAE
                     MERLKKLGLEVITYDQFKLLKNERVLIRAHGEPPSTYEIAAQNNLTLLDYSCPVVLKL
                     QKRIKDGFDKSQKQNGQIVIYGKPGHAEVIGLNGQIDDQAIIISSPENLEGINFSKPV
                     VLFSQTTKDETGFYRTAENIRAGMISAGQNPDNNLIINNTICRLVSKRVPQLRKFAAE
                     HDIILFVSGIKSSNGQFLFSICLEVNKRCYLVPDVSYLQKEWFKQNDSVGVCGATSTP
                     MWLMEEVAEAVKKITLKK"
     gene            10756..11937
                     /locus_tag="PKY63_09955"
     CDS             10756..11937
                     /locus_tag="PKY63_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00979.1"
                     /translation="MNNFPAHTANIQTHSFHIPVMGIGYTIDTATRVAPYGIDSVASL
                     LDDGLMERLRKKLCSVHNQPYEEIAKTEDDCRAKRTTAYLNLLKFISEKRFEELINNP
                     GSVEKYFSLLPAESELNKRYKEFRQTNPSEEETGQWLKNNLRLGSIDVNIMTKLDKIN
                     YKNGERLPTERNDAHSALRGFANSDLESSIVFSAGMNPSLYGYIQQFSDFFPDEKGYI
                     RKKVTIKVSDYRSALIQGKFLAKKGVWVSEYRMESGLNCGGHAFATDGFLMGPILEEF
                     RKNREELYKTVFSILQEALASKNLPVPDSLPMRITAQGGVGTAEEHDFLLEYYQLDSI
                     GWGTPFLLVPEVTNVDDFTLQKMIDAKEKDLYLSDISPLGVQFNNLRASLKTQKPHQH
                     C"
BASE COUNT         3339 a         2585 c         2454 g         3470 t
ORIGIN      
        1 gacccgagta gaaatattcg tcagtaatat ttatcgggat aattcattat aagtatattt
       61 gtgagtgttt aaaagccaga ataaattctg aagaattatt aaatgaaaag aatcctgttc
      121 tcattctgtc ttatcgctgg tttgatgacc gcattgtatg cacaattgta cacaggaggc
      181 tataaatcat tcaatgattc tgctttttca aaaggagata aaattctggc tcctaaaatt
      241 ttgtttaccc tgagcggagg ttcacgcgtt cttcctgaat gcgaagattc ggtaaaaata
      301 attgccgatt ttctgaatga gcatcccgaa atgtgcattg aaattggtgt gcacaccgat
      361 taccgcggcg cggatgaatt caatgacacc atttcatatc atcgggctgg atcagttaaa
      421 gaagtactcg ttggtttgtt tgaaattgat ccgaccagaa ttgaaataaa aggatacggt
      481 aaacaacaac cacttatttc aaaagaagaa atcgacgctg ttcaatccaa agaaatgcag
      541 gaaaccatgc accgcatcaa ccgccgggtt gaagtaaaaa ttattcagat tcaacaataa
      601 aacactatat gagaattgac atcaaccaga aaaaaatttc gatcggagat aagtatcaga
      661 tttttataaa tggccagcag tgctattccg gagcgaaaaa gcttttcaga tttctggcca
      721 aaattgaatt gtttgatcaa ttgaatcagc aggtcaaact caccattaaa aagaaattga
      781 gcttgtttcg tgcaaaattc agaatcattt tccccatcgg tacagacatt caattcgaaa
      841 ctgtttcata ctggaaactt cattatcgct gtgttttaaa caaagacacc tatgagatat
      901 acggacatcg cagccgcaaa tattctgtgt ataaaaacgg tattcagatc gcctggtggg
      961 ataaggaagt tgtttcttgg tttaatggcg acaactacac cattgtggcc gacaacgatt
     1021 gcaactacga gctgatcatc gcattctgcc tcattattga tgagttccgc tccgacgata
     1081 ataaaagcgc ggtgaatgtc gatattggcc acatcggtcc gcaggccacg ccattcaatc
     1141 cgaactggca gccgaaaaac tgatgctgtg agtgcagctt tcagcaagtc gattcgccat
     1201 cggtgagcag cgagcggctg aaaaatacga gatacaaaat tcgaaataaa agattagaat
     1261 caggcgtccg ccaccgcgga tgagaaggag ttttggacag agagagagta aagctcagcc
     1321 atgcaccatg tcttatgcta aatgattctt gcttcttcaa cgcactcagg tcgctttgcg
     1381 acgcagagag gttgaagaaa aaccgcagaa attaatcact ttcaaatttt caaatgagca
     1441 catgagcacg tcatttcttc tttaccgaat agccaaacgt tccaaccaga tcgccatgct
     1501 cgaagtatcc gccatgattg taatttatca gagcggcacg ttctatttcg aatttagaag
     1561 ttcttgtgtt gaagaatctt tcgtcggcaa tggtgttaag cacttcggcc acgaacatgt
     1621 ggtgcgaacc aagttcgata atttgtgtca ctttgcattc catcgacagc gggctttcgg
     1681 caatatacgg acaaggaatc tgaattccgg ccacaggcgt gagtttcatg gttttaaact
     1741 tatccagatc ctttccgctc ctgactccgc aaaagtcggc tgctttcacc aggtttttat
     1801 ccaccagatt aatcgtaaac gccatttgtt cttttatcat tgcatatgaa tacctttcag
     1861 gccgcacaga gatgtacacc atcggtggag tcgtacacaa agtaccggtc catgcaatgg
     1921 taatgatatt gtgcttctcg ctgttaccgc atgaaaccat gactaccggc agggggtaca
     1981 acatgttgcc gggtttccag atatgttttt cctgaatcat ttttatgtcg tttttgcaaa
     2041 gataatcttt tgatgacttt gtcggctacg caagtcaaaa ctccagctgt gagcggtgag
     2101 tggtgagtgg tgaccataca actcatcgga gcttcgcaca ccttgtccct ggggcttcgc
     2161 ccgaggctat tcttatttaa ctcctccgga gtatccggtg gtgctgcgca gctttatttg
     2221 atgccttcgg acacaacaga ttttcttatg ttgggcgcgc gtctcctgac gcgtgaccta
     2281 ccaactcaaa ggagcttcgc gtaatacgag acatgtcgca actttctttg acgccttcgg
     2341 ccacaattac gttgtacttt gcgcattgtg cgtggaattt atcacgctga aggcggaagg
     2401 cgttacacgt tacaattgaa aaaaataagg ttcccgaccg gaacaggaat cagcactata
     2461 gagtaaatgg cttgtgcaga gccgtaatct ccttaaaacc ttcgacttgt catcacgtga
     2521 aaccacgtgg cacgtggcaa gctgtgacaa gctgttttac gagaagcatt tccatttttc
     2581 ttccgcgaat ccgcgcaacg aagaataccg tttcaaccgt gtttttaaat tttaaatgca
     2641 acttgtacca aaattcgtat atttgggtca aataaaaaat acatgggaca aaatgcaccg
     2701 cgtttcaaat tagaaaaact tcctccgttg agagagaaag ttgaaacgat tgaaatactt
     2761 cggcaaacga acaaatcaac ggcggtactg gctgaactga aaggggtcgc gaaaacaatt
     2821 cccaatcagg ccatgttaat caatgccatc gttttgcagg aagctaaaga tagttccgaa
     2881 attgagaata ttattacaac tcaggatgaa ttgtatcaag ctcttgcaat taataaaccc
     2941 catatttcta ccgaaataaa agaagttgta aattaccgca aagcaatatt ccatggtttt
     3001 gatcttttaa aaaaacaaga gtttctgaga gtgaatgata tcataagcat tcagcagaaa
     3061 ttggtggata atacagctgg aataagaagc accccgggaa ctgtactgaa aaacgataaa
     3121 accggagatg tggtttatac accaccacag gacaaagcag aaatattgga tttgttatca
     3181 aattttattg aacattttaa tcattcaggt gatttatcac ccctgattaa cctggctgtt
     3241 tttcattatc agttcgaaag catacaccct ttttatgatg gaaacgggag aaccggccgc
     3301 atcctgaata ttttgtattt gattttgaac aagttgatag atgtacctat tttatatctc
     3361 agctcataca taattgacca taaaccggaa tactaccgcc tgctcaacca aacgaattca
     3421 tcaggtaact ggcaggagtg gatactgttt atgctgaaag cggtcgaaag tacatcaaaa
     3481 gatacaatta caaaaattct gaatattaaa aatcaacttg attcaacaat tttaaaggtt
     3541 caggaaaaag ctccgaaaat atacaggaaa gaacttgtgg aattgctgtt tgaacaacct
     3601 tattcaaaga ttgaatttgt ggttcataaa cttggtgtgg aaagaaaagc cgcgtcacgg
     3661 tatttgaggg aattggaaat gattggaatt gttgagtcaa aaaaaatcgg gcgtgaaacc
     3721 atttatgtta atcgtgacct gatagaaatc ttaaaaaaat aacacacgcg ccacaaccca
     3781 tcgcgcccct cataaaacct aatctctcct gagatgtttt tgaaaaaaat gcgcgttggt
     3841 gaacaaataa aaatcatgtt tgatcgcatt tggaaaatgt ttgtattttt gaaattgata
     3901 tagcaattgg aattcatatt catccataaa gggaattgat gtctgtattt gacaattcga
     3961 taaaaacagt gtgtgaggta aagaactctt ccatgaaact ttttctgtgt gtactgataa
     4021 ttctcggatt tgcatcctgt tcattatttc ataaaaaaac aataaataat acaaaaccga
     4081 tcaatgattc agcttatatt aattatccca tgaatgccga caccaggttt tttatgtcga
     4141 tgacggaatt catgcataca atagaattag aaaaactttg cgacccggtt cgcatattta
     4201 ttggattttc agttgataca gccgggaaaa tttctaacgc cgattttatt ccatgtattt
     4261 ttgaaaatga tgattgctca attgactcag cttatttgga aaaattacaa gtggaatttg
     4321 aaacaagtat gcctgtatgg aagcctgaaa taaataatga ctcaattaca aaaaccaggt
     4381 acataattcc ggttatattt gattaaagat agatgcacac cacacaatta atctttaggg
     4441 ctgatatgta atccgaggca cggaatcttc ctctgcctat tccgccactt tccgcgcctc
     4501 actcaccgaa acccgcttcc catcaacata agcaaccaca aacgcatcag tcaaaccttt
     4561 ggcgttgagt tcgtttttct ttgcttcagc atcggaatac ttgctgaaat tgccaaccgt
     4621 gtacactgtc agacccgcat cgttcctggt ggtttcgatg ccctgcccgg ctaaggcaat
     4681 gagttcattc acgacattca ccggcacatc tttgctgtag gcgccgatct gcactttgaa
     4741 aacgggcttt gaggcgggcg taatattcgt cggcgtaacc tgtgtattct gcggctgggt
     4801 gccggctccg gactgtttca atccgccacc gaatgtcacg ccgtcggcca tcagctgatt
     4861 ggcttcggcc tgtgtgattt tctttccttt gtacagaatg accacaaacg catcgggcac
     4921 accgcctgcg cggatcttgt tgcgggccgc cacagcgtcg ttcatgttgt tgaaagtacc
     4981 ggcgtaatag cggtaatatt tattgtccat ccgctcctca atcaaaggac tgatgccgaa
     5041 aagcgaggtg ctgttgcgcg tggtggcata cacacccacc tgcacggaat acaccagacc
     5101 ttccacatcg gtcatgctga aattgcctgc tgatgcggcc tgcgctgtta cagccggcgt
     5161 tccgccctgc tccattctcc gggcttcgta caccggaacg cgcttaccat cgaaatatgc
     5221 aaccacaaag gcatcggcat aaccctgctg cctcacccgc ttcagatcag tattggcaat
     5281 gttgtacgaa gtgaaatatc cggtcatgta ctgattccat ccgccggttt tatccacaac
     5341 gacattcatg cctttgaaat atttcgtggt atccacttcc accctcgatg ccgaaatctg
     5401 aatgcggtaa tacaggcggt tgtcagattt tatagtacta ctgtcatcac caacgcttcg
     5461 gtctgtgctg tcagctggca actggctact gacaactgac aactggttat tatcgacaac
     5521 cacattattg tttacaacgt tattgttttg attgttgttc tgattattat tctgattaat
     5581 gttttgatta ttgacctgat tgttgttctg attgttatca gcaacatcct gattggtcac
     5641 agctgacttc tcgtaagcag ccagcagagc tgtatttgaa ggatactgct ttgtgatttc
     5701 agcaatatcg tttccggttg tattttcaat ttcagaaacg gtacggctga caattttttc
     5761 aggatcgtca atgttgtttg ctgccattgc tttctgctgc agatcaaggg actgctgttc
     5821 cttttcctgc gcctgcgcaa gaatgtcgcg ctgttttgca aaatcgtttt cacgataggc
     5881 ctggcttcgc agctgacgtg cttcgatcat aagcatacgc gcattgtcgc tttcctgtga
     5941 tctcgctgca ttgctttcag tcagcgccac attgttgttt tcgtattcgc tgttattcca
     6001 gatgccgtac agatcattcg ccatcaggaa gtgacgtttg gaagattcgg tatattcatc
     6061 cgcctgcttc tgaaatttgg ttttcactgc cgggtcggtg gtggcatcag ccaaacggcg
     6121 cacactgtca gctttgtgca attctgtttc gtacatcagc tgatgaaagc cgatcactga
     6181 ttcttcggaa tacaccggaa tctgctccgg cagctgtttc ccgataagct tcagattatt
     6241 ttcagcgttg tttttataag cgtcggcttc ggccgagtag gcctgcatgg ttttggtcag
     6301 cgctgcaatt tcgtttttga tctgattcga atcggctgga tttgtagcca gctttttctt
     6361 cagcgaagca atgcggacca tgaatgtctg cgctgcggac tcgtaaaacg aagactgaat
     6421 cagatcctgc tcttcgtcgc tggcagaaac cttgtccgaa gcattgctga cctggacatt
     6481 ctgattgttg ttgtcagcaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 ngtcagcgcg ttttgcttct ctttggtgtc ggctatgtca ttctcaatct gcgtgcgctt
     6661 tttcgggttg tcggtgtttt caagtttttc attcagcacc tgaagttttt tctcaacgat
     6721 gttcagttgc ggctgcagct cattcctttc tttttctgtt ttgctgattt ccgaccggaa
     6781 gtcgcctgta cgcgtcgaaa gcagcttttc agtatcatcc gcagcaggac gtgatgtctc
     6841 aagaatggct tcgcttctgg tctgcatctt tttcaactca tttgtatcgg cagctgcttt
     6901 gtccgaaagt tcctgcatga gtttgtcaaa gtcgctttca gcctgagtca gcagtgcatt
     6961 ctgtttattc agttcatcgt gatagtcgct gagcacattc atctgcatgt tgatttcggt
     7021 caggtctgct tcaagctcgg tttttttcat ttgttcgctc tgtgtgagaa ctggcttgtc
     7081 gcgcagtttt acccattccg atagtttgaa atcgtaatcg cttttctttt tggcgaatgc
     7141 aagactcaca gcttcggact cgcgcttatt gcgatccaga cagttctgca cggaatcggc
     7201 tgaagaagaa atttgctgta taatactcgt caccaactcc gttgactgct gattattatc
     7261 tgtaattacc tggttggtgt cggtagcttg ctgattgtta ttgtcggcaa tattctggtt
     7321 gtttacatca gtgttattct gattattgtt atcagcaatg ttttgattgt tgttgtccgt
     7381 tacattctga ttactgacat ctgattgctg attattgttg tccgaatcac tgttcagtga
     7441 atcagcataa gcaactgcat cgctcacgat atcgacacgc tgatcagatt tcgatttcag
     7501 atcgaccatt tcattctgct tcagcttagc atcatctttg agttgctggg ctttgagtcg
     7561 tttgtcattg gcttcccttc tgattttatc agcttcggac tgatactctt ctttcagctc
     7621 aggatcggtt gtcagagcag cttcgttgat atacacttcg gcatccttgt ctaatttttt
     7681 tgcgtcttcc tccagaattt tagattccgc cagatcagac gcaacaacct tttcaagttc
     7741 tgccacatat tcgttatcac cgcggatttt tttatcaagc tgcaccagat tgattttgaa
     7801 agtatcaatt ccatcaatca aatccttgag caaaaccacc gatgtatcga tttgtcgcga
     7861 caaagccagt ttctgtacat taccggcctt atcctgaata cggtcaaaat tctttgcctg
     7921 cacgtcggca gccgcagcat actgattata catctgtttt gccagctcgg attcctcgga
     7981 aagcaacatt gattttttcg acagttcatt agactgagcc atcatatcct tgcgcttttt
     8041 ggcatccgtt tcattcacag acttttcgta caaatcactg ctttgtttgc tgagagcggc
     8101 cgcctgatct tttttctgtt ccgaaatttt caatgatgca tcgcgcatgt tgcggaaaac
     8161 atcgagctga tcttcagcag aagcaacatg ttcaaacgca atatccacaa cctgatcacc
     8221 ggtcggattt tccggaatat tgtattttga gttattattg actgcaatga ctttcgcagt
     8281 atcaataact atttcctcat tgctgtttac atccagactt gccttttcgc gcagttttgc
     8341 aatggtttca agatattcag cactgttaac atctatgttt cctgcagcaa ttagctcagg
     8401 attataattt tcataatccg gaattttacg gtcgatacga accagaaaca ccatcatacg
     8461 accatcggaa ctgtttctta atgacgaaaa ccaggcataa cgctgactcg ggtcagtgat
     8521 aaaaagaata tcgtcaaacg gagagttgat ggcgaaatcg acattgacgg gtgcagtcca
     8581 gtcttttttg acagtatcat acactgtgcg gaaaatatca tatccaccca ttgaattatg
     8641 gcctttcgaa caaaaataga gtgtccggcc gtcgggcaac agatatggat agttttcatc
     8701 gtattccgta ttgacagcac tgctcagttt ttccggcgca gaaaattttc cacccgggag
     8761 cttatacacc ctgtaaatat cgcggccgtt catgtttttt ttgccatagc tgctataata
     8821 cactacctga tgtgtatcgc tgatgaaaac cagctctgga tcagccgaac gacggtccgg
     8881 cttggttttg agaaactctg gtttgggtcc gattcgtcca ccgaatttgg aaatatcata
     8941 cgaacgaaag aaactttcag tcccaacttc gtattttttc atcactacga ggtcgctcag
     9001 attctgcaac agcgtaatac cattggagca catctgattt cgaataaagg cctcttttgc
     9061 ataaggactt ttggacggga cttttccttt aaatgcctca aaagcgcgca gcgcctcacc
     9121 aaagcgatag gcctgatgat aagccacccc cagatgaaaa taaatcatat acgaatcttc
     9181 tcctgaaagc tgaccaacaa aaggttccat gtatttcagc ggtgcttcgg gatcgcgacg
     9241 atcggtataa agaagacaaa ccccaaagcg ataggaataa tatgggtcct ttcgatgaat
     9301 actaagcaat tgcgaataca gcggaaaagc ttctccgtat ttctgttcaa caaacaaccg
     9361 atcagcttcg gtttttacct gcaggtcgtc gcccacaggt tggctcaatg cctcaaaacc
     9421 cgacaacatg aagatgcatg aaatcagata gatgaaaaat atgtttttga cagtaggcaa
     9481 tgctttattt aatcacgcgt gaatggattc tttatacgga ttccggacag ttttgtttct
     9541 ttacaaaggt aagagatgta tttgaatccg gattttcata atcgagacct atccctcgaa
     9601 gggtttggaa gccttcgagg gatttggcga aaattcttac cttcgcaaag caatgaaaat
     9661 taccatcgac agcaaatcgg gcttttgcgc aggcgttaca tcggccattc atggcgctga
     9721 agaagaattg aaagtgaacc agaaactata ctgtctcggc gaccttgttc acaacagtgc
     9781 cgaaatggaa cgtctgaaaa aactggggct ggaagtaatc acttacgacc agttcaaact
     9841 gcttaaaaac gaacgggttc ttataagagc gcatggagag ccgccttcga catatgaaat
     9901 tgcagcacaa aacaatctta cactgctcga ttacagctgt ccggtcgttt taaaattgca
     9961 aaagaggatt aaggatggat ttgacaaatc gcagaaacaa aacggtcaga ttgttattta
    10021 cggaaaacca ggccatgctg aagtcatagg attgaacggc cagattgatg atcaggccat
    10081 catcattagt tcacccgaaa atcttgaagg aatcaatttt tccaaaccag ttgttttgtt
    10141 ttcgcaaaca acaaaagatg aaacaggatt ttatcggaca gcagaaaata tacgggcagg
    10201 catgataagc gcagggcaaa atcccgataa caacctgatc atcaacaata ccatatgccg
    10261 gcttgtttcg aaacgggtac cccagctcag aaaatttgca gccgaacacg atatcatcct
    10321 tttcgtgagt ggaatcaaaa gttccaatgg tcaatttctg ttcagcatct gccttgaagt
    10381 gaataaacgc tgttatctgg ttcccgacgt ttcgtatctt cagaaagaat ggtttaaaca
    10441 aaacgacagt gttggagttt gcggagcaac ctctaccccc atgtggctga tggaagaagt
    10501 ggctgaagct gtaaaaaaaa taacgctgaa aaaataaaaa tcaaacctga aaactgcaat
    10561 tcaagatatg acaacagccc gatcctgtca taatttcact gtattctatt tgttagaatc
    10621 ttgctttcat caacaattca gacaatttcg gaaataatgc ctatctttgc atggaatttt
    10681 aataacggca gttttgtttt cttttttatt gtctttctgt ctggcaaaca catgtagcca
    10741 aaatacattt ttctgatgaa taatttcccc gcacacacag caaacataca aacacattca
    10801 ttccatattc cggtcatggg aattggttat acaattgata cagcaacccg tgttgcacca
    10861 tatggtattg attctgtagc atcactgctc gatgatggcc tgatggaaag actgcgaaaa
    10921 aaattatgtt cggttcacaa tcagccctac gaagagatag caaaaacaga agatgattgc
    10981 agggcaaagc gaacgacagc ttatctcaac ctgctcaaat ttatttcaga aaaacgattc
    11041 gaggaattga ttaataatcc gggatcggtt gaaaaatact ttagtcttct tccggctgaa
    11101 tcagaactca acaaacgata caaggaattc cgccagacaa atccaagcga agaagaaacc
    11161 ggacaatggc tgaaaaacaa tttgcgactt ggaagcattg atgtaaacat catgaccaag
    11221 ctcgacaaga ttaattacaa aaacggcgaa cgtcttccga ccgaacgcaa cgatgcccat
    11281 tctgcattgc gcggatttgc caacagtgat ctggaatcat ccatcgtgtt ttctgctgga
    11341 atgaaccctt cactttacgg atatattcag caattcagcg atttttttcc tgatgaaaaa
    11401 ggttatatcc gaaagaaagt aaccatcaaa gtaagtgatt accgctctgc attgattcag
    11461 ggaaaatttc tggctaaaaa aggcgtttgg gtttcagaat accgcatgga atccgggtta
    11521 aattgtggcg gacatgcttt tgcaaccgat ggttttctga tgggccccat ccttgaagaa
    11581 ttcagaaaaa accgcgaaga actttacaaa actgtttttt ctattctaca ggaagctctt
    11641 gcctccaaaa acctaccggt tcctgatagc cttccaatgc gtattacagc tcagggcggc
    11701 gttgggacag ccgaagaaca cgatttttta ctcgaatact accagcttga ttccattggc
    11761 tggggcaccc catttctgct cgttcccgaa gtaaccaatg tggatgattt cacattgcag
    11821 aaaatgattg atgccaaaga aaaagattta tacctcagcg atatctcacc tttaggtgtt
    11881 caattcaata atttaagggc atctctaaaa actcaaaaac cgcatcaaca ctgttgataa
    11941 aggctgtt
//
LOCUS       DAOPKE010000083        11805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5706_length_11805_cov_12.631745, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000083 DAOPKE010000000
VERSION     DAOPKE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_11805_cov_12.631745"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKY63_09960"
     CDS             <1..447
                     /locus_tag="PKY63_09960"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPF00980.1"
                     /translation="MVCLGNICRTPIAVGLLRKKLAEIGSSSEVDSCGFEPHHIGDPA
                     DTRALKVMAEKGIDISAHRARLFHPDDFKNNDLIFAMDTFILADIKRMAKNEDDLKKV
                     DLLLNVQYPGMNCEVPDPYYGDAGGFHKTFEIIDKALDALIEKYEK"
     gene            444..1400
                     /locus_tag="PKY63_09965"
     CDS             444..1400
                     /locus_tag="PKY63_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPF00981.1"
                     /translation="MIPRQFADKSVFTVAANRIKDYIQHTPLLSCSAINHICGCQIVF
                     KCENFQKAGAFKSRGAANAVFSLIESGFNGSVATHSSGNHAQALSRVALHTGIPAHVV
                     MPENSLTSKIHATRDYKAQITFCKPTLEARESTLREVMAASGAVEIHPYDNIEIIAGQ
                     ATCAMEILEETQPDFIIAPVGGGGLLSGTALAAAHFGQCVRVIGAEPAGAADAYESFK
                     TKAFVPSVNPKTIADGLLTSLGQITFPVILAQVSDILLAGEEQIIDAMRLLWERAKIM
                     AEPSSALPLAVVKANPGIFKNKKVCIIISGGNADLDHLPWLK"
     gene            1388..2104
                     /locus_tag="PKY63_09970"
     CDS             1388..2104
                     /locus_tag="PKY63_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPF00982.1"
                     /translation="MAEIKKMYLIPVDLGHSPEGGIYAPSQLMIIHEIKHFVAENAST
                     ARKMLKKCNYPHPLSEAEIIEFNEHTRAFPETMPDVKKMLSENNDIGLMSEAGMPCVA
                     DPGHEVVRLAHDMGIQVIPMHGPSSIMQALVASGLQGQQFVFHGYLPAKTDARKARLR
                     ELQQKAMRDHYTHIFMEAPYRSNQMLHDILESFGEHFSICVAAEIGTPAEFIHTKKTG
                     QWKKLLPDLHKKRCIFCVSA"
     gene            complement(2101..2607)
                     /locus_tag="PKY63_09975"
     CDS             complement(2101..2607)
                     /locus_tag="PKY63_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPF00983.1"
                     /translation="MKIKKNKVVSLLYQLSENDQNGNVIEAVDESRPFTFLFGNGQLI
                     PGFENNVANLSVGESFGFQVLPEDGYGTYEQENIADLPISIFMRDGKVDTEILKVGNM
                     VPLQDDKGHVFHGMVKEIGLEKVNIDFNHPLAGKTLFFSGKILEVRDASEEELAHGHV
                     HGPNGHQH"
     gene            complement(2634..7175)
                     /locus_tag="PKY63_09980"
     CDS             complement(2634..7175)
                     /locus_tag="PKY63_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00984.1"
                     /translation="MKLAISLLALLLTQNIFAQLKRFSDDPNKFLAEMTSMYTILENK
                     DSRKAGELFMLEKFTPFWNSGVLVEPQKKTVITNCNLMLKKKMKAYPHFMNYMVSLMN
                     MSEAGKLPAVFDPWHQTLDKLMGKSTSNSFLNFLSASDSLFLQNYLYVSNTTKWVASN
                     STYDFTFEDEPVVFFPSLILTCYANNDSSVIYDTRGKYYPLQKKWVGEKGQIDWIRAG
                     LEPTEVYATFGHYEIELKSREYIVDTVLFYNKEFFGATPLPGRIEEKVLANRSGDRAL
                     YPQFTSFDKRLTIKEIYKNVDYEGGFSMHGPKLLGSGDEIYDAILSFKKDGTVFLVSR
                     SKSFSIRRDRVNSDRASVSIYFEGDSIYHPGLIMRYIDKKLNPEDLAKGDSVWVSVKE
                     ISFIRDKEGLRQTPFFNTYHKVDMHFEALYWKMEDPIIDFRMIKGPGSSSEALFESAN
                     YYSEGRFEKLKGINDLHPFQSLKNYSNQIGSRVFPVADYANFRRIQVEGIRGEIIDYA
                     IKGFLIYDPNTDKVFIKDKVFDYLNAKVGKADYDVIQFHSIIEAESNATFSLLNWDLR
                     IKGVSRVFLSDSQAVYIYPADQEIVVKKNRDFTFAGRVHAGMFDYYGKNFSFEYDKFM
                     LNLPIIDSMTFKVKSRKPDEYGEYPLIRVKSVIEDLSGDILIDHPNNKSGRKPFPEYP
                     IFNSKKFSFVYWDKNSDYPGSYPRENFFYRIDPFTLDSLDDFSTDGLEFSGYLSSAGI
                     FPDIAEPLKVMKDYSLGFIHNTGPAGYPAYGGKGMFTNIIDLSNKGLYGDGTLTYLSS
                     ESKSDNFLFFPDYMTAKVRDYNISEQSGANSIPPVEAHNVQQEWRPYEDRLSVTTTDQ
                     PARMYAEETEMNGTLNITPGGLTGTGKLDFRNATMTSNLFKFKNRTFDTDTCDFYLKT
                     ADYDELAFETKNYKGHVDFDEKKGEFKSNGGTSLVNFPVNEYICYMDQFDWYMDKDEI
                     ELQNNAVNVPGLDKMDIKQLADVDLAGSEFISTHPAQDSLRFRSSRAKYSLREKIIYA
                     SDVMFIKVADATIFPGDGKVTILKKAEIVPLENAQILANNTTKYHTISSAKVSIYGKN
                     SYLGEGYYDYIDEMEGMQRVFMDKVSVDSTIQTYAHGYISDSIEFTLSPHFEYYGDVS
                     LTASAEFLTFDGGVRIKHQCDTLGLQWLKFTAEINPMEIFIPVGETLTNTKGDRIYLG
                     LYLNRDSIGVYPAFFSQKYLGGDPELASSSGYLTYDHSNNEYRVSSKEKLAQPVLPGK
                     YMSLSVFKCMSRNEGKLSFGDNFGRMKMNNYGVIDHSNRDSSTMIYTVSELDFFLAPA
                     ALEIFEQSFAANPDLEPTNVRSELYTKYLGEIMNAEDAEKAESELVLYGYFKKLPEKL
                     EHTITFSDLTLKYNEKTKSYISQGKIGISTVGKIQVNKYVDGKMELSQKRSGDRLTFY
                     LDIGSDWFFFDYYNGLMSVFSSKKEYNDIVINTKDEDRELKAKDGEKAYRFYISTTSR
                     KNKFLKKINDGSEEMEEGE"
     gene            complement(7182..8015)
                     /gene="prmA"
                     /locus_tag="PKY63_09985"
     CDS             complement(7182..8015)
                     /gene="prmA"
                     /locus_tag="PKY63_09985"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPF00985.1"
                     /translation="MLQITLTGLPKDDDTRELVMSGMLELGFDSFWEDGALLHGYIAA
                     ENYDADSLNMMIENKLPNSHVRIIQAELENKNWNEEWEKNFPPTEIEGQIYVSAPFHP
                     QRPEIPYQIRIVPKMSFGTAQHATTYSMLRLMIEMDFGNKTVIDAGTGTGILAIYAEM
                     KNAASVFAYDNDDWSVENSIENIERNHCSKIEIAKGEKEILTGKSCDILLANIHKNVI
                     LGDLPFYHNALKTGGDLLLSGFYSEDLPDISDACEALGMEIEKFIVKENWVAARFTKV
                     K"
     gene            complement(8031..8789)
                     /gene="tpiA"
                     /locus_tag="PKY63_09990"
     CDS             complement(8031..8789)
                     /gene="tpiA"
                     /locus_tag="PKY63_09990"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPF00986.1"
                     /translation="MRRKLVAGNWKMNQDTAGIKTFASELKSNLVDFDGRADMLICPP
                     FVYLPLLQELFAGTAVAIGAQNVASEEKGAYTGEVSAAMLKDLALPYCIIGHSERRQY
                     YHESDELLVKKIQLLFTNGIKPVFCCGEMLPEREAGNHFNIVQSQLEGALWNLPEEIM
                     TTITIAYEPVWAIGTGVTATPAQAQEMHAFIRELIEKRFGSDVSANIRILYGGSVTPD
                     NATELFSMADIDGGLVGGASLKAESFTKLINSCQ"
     gene            complement(9022..9870)
                     /locus_tag="PKY63_09995"
     CDS             complement(9022..9870)
                     /locus_tag="PKY63_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HPF00987.1"
                     /translation="MAYNLLKGKKGIIFGALNDQSIAWKVAERAHEEGAQIVLSNTAV
                     AMRLGNVDELAEKCSSIVVPADATNAADVENLIAKSMDFFGGKFDFILHSIGMSPNVR
                     KGIPYDNINYENYLKTIDISALSFHKVIQMCRKMDAINDWGSIVALSYVAAQRTLYGY
                     NDMADAKALLESIARSFGYIYGREKKIRINTISQSPTPTTAGSGVFGFQSLLDFTDRM
                     SPLGNASADDCADYCITLFSDLTKKVTMQNLFHDGGFSSMGMSESAMTQYDKSFKDCD
                     ELKKKE"
     gene            complement(10366..11112)
                     /locus_tag="PKY63_10000"
     CDS             complement(10366..11112)
                     /locus_tag="PKY63_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF00988.1"
                     /translation="MISGKKVVVVFPAYNAEKTIELTYSEIPFDIVDDVILVDDCSRD
                     RTSEVAARLGIRHIIRHETNKGYGGNQKTCYDKALELGADIVVMLHPDYQYTPKLVSP
                     MVHIIAEGLYPVVFGSRILGRGARKGGMPLYKYIFNRILTLTENLIIGQKLSEYHTGY
                     RTFSADVLRKINYHANSDDFVFDNQMIAQIFMAGFDIAEVTCPTKYFDEASSINFRRS
                     MKYGFGVLGTAFQYRFHKWGLCKSGLFTGK"
BASE COUNT         3170 a         2564 c         2554 g         3517 t
ORIGIN      
        1 atggtttgtc tgggcaacat ttgccgtact cctattgcag ttgggctgct gcgcaaaaaa
       61 ttagctgaaa ttggtagtag ttccgaagtt gattcctgtg gatttgaacc gcatcatatt
      121 ggtgatcctg ccgatacacg tgctttgaaa gtaatggccg aaaaaggcat tgatatcagc
      181 gctcatcgtg cccgcctctt tcatcctgat gattttaaaa acaacgatct gatatttgcc
      241 atggatactt tcatacttgc cgacattaag cgtatggcca aaaatgaaga cgatctgaaa
      301 aaagtcgatt tgctgctcaa tgttcagtat ccaggcatga actgcgaagt tcctgatccc
      361 tattatggtg atgcaggcgg atttcacaag accttcgaaa taatcgacaa agcgttggat
      421 gctctcattg agaaatatga gaaatgatac cccgacagtt tgcagataaa tctgttttta
      481 ctgttgcagc caacagaatc aaggattaca ttcagcatac accactattg tcgtgttcgg
      541 caatcaatca tatctgcggt tgtcaaattg tttttaaatg cgaaaatttt cagaaggccg
      601 gtgcattcaa atcccggggt gcggccaatg ctgttttttc actgattgaa agtggcttta
      661 atggcagtgt agccacccat tcttcgggca atcatgctca ggccctgagt cgtgtcgctt
      721 tgcataccgg tattccagct catgtggtta tgcctgaaaa ttcactgacc tcaaaaattc
      781 atgctacacg cgattataag gcacaaatta ccttttgcaa accaacgctt gaagcgagag
      841 aatctacact gcgtgaagtg atggctgcat cgggtgcggt tgaaattcat ccttacgaca
      901 acattgaaat cattgctggg caggctacct gcgctatgga aattctcgaa gaaactcaac
      961 ccgatttcat tattgcacct gtgggtggcg gaggattact gagcggaaca gctctggcag
     1021 cagcgcattt tggacaatgc gtgagggtta ttggagctga gccggctggt gctgccgatg
     1081 cttatgaatc attcaaaaca aaggcattcg ttccttcggt taatccaaaa acaatagccg
     1141 atggtttact tacttcgctc ggacaaatta cctttccggt tattctggcg caggtgagtg
     1201 atattttatt ggccggagaa gagcaaatta ttgatgccat gcgcttactt tgggaaagag
     1261 ccaaaattat ggctgaacct tcttcggcat tgccgctggc tgttgtaaaa gccaatcccg
     1321 gtatttttaa aaacaagaaa gtatgtatta ttatttccgg cggtaacgcc gatcttgatc
     1381 atttgccatg gctgaaataa aaaaaatgta tctgattcct gtcgatctcg gtcattcgcc
     1441 tgaaggtggc atttatgccc cttcgcaatt aatgattatt cacgagatca agcattttgt
     1501 tgcagaaaat gcatccacag cccgtaaaat gcttaaaaaa tgcaattacc cgcatccatt
     1561 gtccgaagct gaaattattg aattcaacga gcatacacgg gcctttccgg aaacgatgcc
     1621 ggacgtgaaa aaaatgcttt cagaaaataa tgatattggg ttaatgtccg aagcggggat
     1681 gccatgtgtg gctgatccgg gtcacgaagt tgtgcgattg gcgcacgata tgggcattca
     1741 ggttattcct atgcatggac catcttcaat tatgcaggcg ctggttgcat cggggctgca
     1801 ggggcagcaa tttgtttttc acggttatct gccagctaaa accgatgcca ggaaagcgcg
     1861 gctcagggag ttgcagcaaa aagctatgag agatcattac acgcatattt ttatggaggc
     1921 gccttatcgt tccaatcaaa tgctgcatga tattctggag tcgtttggtg aacatttcag
     1981 tatttgtgtg gcagcagaaa taggtacccc ggctgaattc atacacacaa aaaaaaccgg
     2041 gcaatggaaa aaattattgc ccgatttgca taaaaagcgc tgtatctttt gtgtttcggc
     2101 ttagtgctga tggccatttg ggccatgcac atgaccatga gccaattctt cttcgctggc
     2161 atcgcgcact tcaagaattt taccactgaa aaagagtgtt tttccggcca gcggatgatt
     2221 gaaatcaata ttcacctttt cgagtccgat ttcttttacc atgccatgaa aaacatgtcc
     2281 tttatcgtct tgcagcggaa ccatatttcc gactttcaga atttcagtat ccactttccc
     2341 atcacgcata aaaatgctga ttggcaggtc ggcaatgttt tcctgttcat atgttccata
     2401 gccatcctcg ggaagaacct gaaaaccgaa tgattcaccg acactcagat tggcaacatt
     2461 gttttcgaag ccgggaatca attgtccgtt tccgaagaga aaagtaaacg gacgcgactc
     2521 atccactgct tcgatgacat taccgttctg gtcgttttct gaaagttggt acaataatga
     2581 aacaactttg ttttttttaa ttttcatttt atttgttttg gtttgtagtt tttttattct
     2641 ccttcttcca tttcttcaga accgtcattg attttcttca gaaatttatt tttacgcgag
     2701 gttgttgaaa tgtagaaccg gtaagctttt tcgccgtctt ttgcctttaa ttcacggtct
     2761 tcgtcttttg tgttgataac aatgtcattg tattcttttt tcgaagaaaa taccgacatg
     2821 agaccattgt aataatcaaa gaaaaaccag tctgagccaa tatcgagata aaatgtgagt
     2881 cggtctccgc tgcgtttttg cgagagttcc attttcccat cgacatattt gtttacctga
     2941 atttttccaa ccgttgatat tccgattttg ccctgtgaaa tatagctttt tgttttttcg
     3001 ttgtatttca gtgtcagatc cgaaaaagtg atggtatgct ccagtttttc gggcagtttt
     3061 ttaaagtaac cgtataaaac aagctctgac tctgcttttt cagcatcttc ggcattcatg
     3121 atttcgccca gatatttggt gtataattca ctccgtacat tggtgggctc cagatcggga
     3181 ttggccgcaa acgactgctc aaatatttca agagcggcag gtgccagaaa gaaatcaagt
     3241 tctgatacgg tgtagatcat ggtgctgctg tcgcggttcg aatggtcgat gacaccgtaa
     3301 ttattcattt tcatgcgccc gaaattatcg ccaaatgaaa gttttccttc gtttcgcgac
     3361 atgcatttaa aaacagacag actcatgtat ttgcctggca aaaccggttg agccagtttt
     3421 tctttcgacg aaacacgata ttcgttgttg ctgtggtcat aggtgagata gccgctcgag
     3481 cttgccagtt cgggatctcc tccgagatat ttctgagaga aaaaagccgg gtaaacacct
     3541 atagaatcac gattgagata aagtccgaga tatattctgt cgcccttggt attcgtgagc
     3601 gtttcaccaa ccggaataaa aatctccatt gggttgattt cagctgtaaa tttaagccat
     3661 tgcaatccca gtgtgtcaca ttgatgcttg attctcacac cgccatcgaa ggtcagaaat
     3721 tcagccgagg ccgtcagact tacatctcca tagtattcaa aatgagggct cagcgtgaat
     3781 tcaattgagt cgctgatata gccatgtgcg taggtttgaa tggttgaatc aaccgacact
     3841 ttgtccatga aaacacgttg catcccttcc atttcatcaa tatagtcgta ataaccttcg
     3901 cccagataag aattttttcc ataaatggag accttggcac tactgatggt gtggtatttt
     3961 gttgtgttgt ttgccagaat ctgtgcgttt tcgagcggaa caatttccgc ttttttcaga
     4021 atcgtcactt ttccgtcgcc tggaaaaata gtggcatcgg caactttgat aaacataaca
     4081 tcgctggcat aaatgatttt ttcgcgcaga ctgtatttgg cgcgcgaaga gcggaaacga
     4141 agcgaatcct gtgcaggatg cgttgagata aattcagaac ccgccaaatc tacatctgcc
     4201 agctgtttta tatccatttt atcgagtccg ggaacattta ccgcattgtt ttgcagctct
     4261 atctcatcct tgtccatata ccagtcaaac tgatccatgt agcagatata ttcattcaca
     4321 ggaaagttta ccagtgatgt accaccgttt gatttgaatt ctcccttttt ctcatcaaaa
     4381 tcaacgtgtc ccttatagtt ttttgtttca aaggccagtt cgtcgtaatc ggccgttttt
     4441 aaataaaaat cacaggtatc ggtgtcgaat gtcctgtttt tgaatttaaa aagattggat
     4501 gtcattgtgg catttctgaa atcaagtttc cctgtaccgg tgagtccgcc gggagtgata
     4561 ttcagtgttc cgttcatttc agtttcttca gcatacatgc gtgccggctg atcggtcgtg
     4621 gttaccgata accgatcctc gtatggtcgc cattcctgtt gtacattgtg cgcttcaacg
     4681 ggtggaatgc tgtttgcgcc actttgttca ctgatattgt aatccctcac ttttgctgtc
     4741 atgtaatccg ggaaaaacag gaaattatcc gatttgcttt cggatgataa atatgttaaa
     4801 gtaccatctc catacaatcc tttattcgat agatcgatta tatttgtaaa cattcctttt
     4861 cctccataag cgggataccc tgccgggcct gtgttgtgga taaatccaag tgagtaatct
     4921 ttcatcactt tgagtggctc tgcgatgtcg ggaaaaatgc cagccgacga caaatagccg
     4981 ctgaattcaa gtccgtcggt cgaaaagtca tcgagactgt caagggtgaa tggatcaatt
     5041 cggtagaaga aattttctct tggataagag cccggataat ccgagttttt atcccaataa
     5101 acaaaagaga attttttgga attgaaaatc ggatattccg gaaatggttt tcgtccggac
     5161 ttgttgtttg ggtgatcaat caggatatct ccgctgagat cttcaataac gctcttgact
     5221 cgaatcaacg ggtattcgcc atattcatcc ggtttgcggc tcttgacctt aaatgtcatg
     5281 gagtcgataa tcggcagatt gagcatgaat ttgtcgtatt cgaacgaaaa gtttttccca
     5341 taataatcaa acatccccgc gtgaacacgt ccggcaaaag taaagtcgcg attttttttc
     5401 acaacaattt cctgatcggc aggataaata taaacggctt gcgaatcgct cagaaacaca
     5461 cgcgaaaccc ctttaatccg caaatcccag ttgagcagac tgaatgtcgc attggattcg
     5521 gcttcaatga ttgaatgaaa ctgaatcaca tcgtaatcgg cttttccaac ttttgcattg
     5581 agataatcaa atactttgtc cttgataaaa accttatcgg tattggggtc ataaatcaaa
     5641 aatcctttga tggcataatc gatgatttcg ccacggatac cttcaacctg aattcttctg
     5701 aaattagcat aatcggccac cgggaatacc cttgatccta tctggttgct gtagttttta
     5761 agcgattgaa aaggatgcag atcattgata cccttgagct tttcaaaacg accttcagaa
     5821 taataatttg ctgattcaaa caaagcttcg ctcgacgaac cgggaccttt gatcattctg
     5881 aaatcaataa tggggtcttc cattttccag tacaatgctt caaaatgcat gtcaacttta
     5941 tgataggtgt tgaaaaatgg cgtttgacgc agcccttcct tatcacggat aaatgaaatt
     6001 tcttttaccg aaacccagac actgtcgcct tttgcaaggt cttctgggtt cagttttttg
     6061 tcgatataac gcatgataag ccccggatga taaatggaat cgccttcaaa gtatatcgaa
     6121 acagaggcgc ggtcgctgtt tacacgatcg cggcgtatgc tgaaactttt cgatcgcgat
     6181 accagaaata cagtgccgtc ttttttgaat gataaaatgg catcgtagat ttcatcacca
     6241 gaaccaagca atttcgggcc gtgcatcgaa aatccgcctt cataatccac atttttataa
     6301 atctctttaa tggtaagtcg tttatcaaat gatgtgaatt gaggatacaa agccctgtcg
     6361 ccgctgcgat tggccagcac tttttcttca atccgtcctg gcaatggtgt tgctccgaaa
     6421 aattccttgt tgtaaaaaag taccgtatca acaatgtatt cccggctttt taattcaatt
     6481 tcgtaatgcc caaacgttgc atatacttct gttggttcca agccggctct gatccagtca
     6541 atttgtccct tttcaccaac ccattttttt tgtagcggat agtattttcc acgcgtgtcg
     6601 tatatcacgg agctgtcgtt attggcatag caggtcagaa tcagcgatgg aaaaaatact
     6661 accggctcat cttcaaatgt aaaatcgtat gtcgaattgc tcgcaaccca ttttgtagtg
     6721 tttgaaacat ataaatagtt ttgcagaaac aacgaatccg aagccgacag aaaattcaga
     6781 aagctattgc tggtcgattt tcccatcagc ttatccaatg tctgatgcca tggatcgaag
     6841 actgcgggca gtttcccggc ttcagacata ttcatgagac tgaccatgta attcataaaa
     6901 tgaggatagg ccttcatttt tttcttcagc atcagattgc agtttgtaat gactgttttt
     6961 ttctgaggtt caacaagcac gccgctgttc cagaatggtg tgaatttttc cagcatgaat
     7021 aattcaccgg ctttgcgact gtctttgttt tccagaatgg tgtacatact ggtcatttct
     7081 gccagaaact tattcgggtc atctgaaaat cgtttcaatt gtgcaaaaat gttttgcgtc
     7141 agcagcaggg caagaagtga aatggctaat ttcataacaa gttatttaac cttggtaaaa
     7201 cgtgcggcaa cccagttttc ttttacaatg aacttttcaa tttccattcc caaagcttcg
     7261 caggcatcgg aaatatcggg cagatcttcg ctgtagaaac cgcttaaaag caaatcccca
     7321 cccgttttca gcgcgttgtg ataaaaaggc agatcgccca gaatcacatt tttatgaatg
     7381 ttggccagca atatatcaca gcttttacct gtcagaattt ctttttcgcc tttggcgatc
     7441 tcaattttgc tgcagtggtt gcgttcaata ttttcgatcg aattttctac cgaccagtca
     7501 tcattgtcgt atgcaaacac cgatgccgca tttttcattt cggcataaat ggccagaatt
     7561 cctgtgccgg taccggcatc aatcactgtt ttatttccga aatccatctc aatcatcaga
     7621 cgcagcatgg agtaggtggt ggcgtgttgc gctgtgccaa acgacatttt cggaacgatg
     7681 cggatctgat atggaatttc aggacgttgc ggatggaatg gtgctgaaac atagatttga
     7741 ccttcgattt cggtaggtgg aaaattcttc tcccattctt cgttccagtt tttattttca
     7801 agttcggctt gaatgattcg tacatggctg ttgggcagct tgttttcaat catcatgttt
     7861 aaagaatctg catcgtagtt ttctgctgca atataaccat gcaaaagtgc cccatcttcc
     7921 cagaagctgt cgaagccaag ttccagcatg ccagacatga cgagttcacg ggtgtcatca
     7981 tccttgggca gcccggtaag agttatttga agcatttgtg gattattcat ttattggcag
     8041 ctgttgatga gttttgtaaa agattctgct ttgagtgagg ccccgcctac cagtccgcca
     8101 tcgatatcgg ccatggagaa cagttctgtt gcgttgtcgg gtgtgacact tccgccgtac
     8161 agtatgcgga tgttggcaga aacatcgctt ccgaagcgct tttcgatgag ttcacggata
     8221 aaggcgtgca tttcctgcgc ctgtgccgga gtagctgtaa cgccggttcc gatggcccac
     8281 accggttcat aggctatggt aatggttgtc attatttctt ccggaagatt ccacagggcg
     8341 ccttccagct ggctttgaac tatgttgaaa tgatttcctg cttcgcgctc gggcaacatc
     8401 tcaccgcagc aaaacacagg cttgatgccg tttgtaaaca acagctgaat tttttttacc
     8461 agcagctcat cgctttcgtg ataatactga cggcgctcgg aatgaccaat gatgcagtag
     8521 ggcagtgcca gatccttcag catagcggcc gaaacttcgc cggtgtaagc gcctttttct
     8581 tcgctggcaa cattctgggc accaatggca accgctgttc cggcaaataa ttcttgcagc
     8641 aaaggcaaat aaacaaacgg aggacaaata agcatatcgg ccctgccatc aaaatcaaca
     8701 agattgcttt taagttccga agcaaaggtt tttataccgg ctgtatcctg attcattttc
     8761 cagttgccgg ctaccagttt tctgcgcata actattcttt ttttgtaagg tacgaaaata
     8821 cgaagaatag ttgaaatggc cttcattatt aaacacgcaa cttttcaaca tgtatcaaag
     8881 gcgatgcagc agaaaaaaag ttacaacatt tggaaattat accaatttga aatatatgat
     8941 aagccaaagg gaaattatat atttcataaa ttggtaaatg ccgacagaat tatttagact
     9001 acaatataat tctcgtcggt attattcctt ctttttcaat tcatcacaat ccttgaaact
     9061 cttgtcgtac tgagtcatgg cgctttcgct catacccatg ctgctgaagc cgccatcgtg
     9121 gaaaagattc tgcatggtga ctttttttgt cagatcggaa aacagagtaa tgcagtaatc
     9181 ggcgcagtcg tcggccgaag cattgcccag cggcgacatg cgatcggtaa aatcgagcag
     9241 cgactgaaag ccgaaaacgc cgctaccggc cgtagtcgga gtaggtgatt gcgatatggt
     9301 attgatgcgg atttttttct cgcgtccgta aatatatccg aagctgcgtg caatggactc
     9361 cagcagcgct ttggcatcgg ccatatcgtt gtagccatat aatgtgcgct gagctgccac
     9421 atacgacaac gccacaatgg agccccaatc gttgatggcg tccattttac ggcacatctg
     9481 aatcactttg tgaaacgaca gcgccgaaat atcaatggtt ttcagataat tttcgtagtt
     9541 gatattgtcg tatggaattc ctttgcgcac attgggcgac atgccgatgg aatgcagaat
     9601 aaaatcgaat ttacctccga aaaaatccat ggattttgca atcagatttt caacatcggc
     9661 ggcgttggtg gcatcggccg gaacaacaat gctgctgcac ttttcagcca gttcatccac
     9721 attacccagg cgcatggcca cggctgtgtt tgaaagaaca atctgtgctc cttcttcatg
     9781 tgctctctcc gccactttcc aggcaataga ctgatcgttg agtgcgccga aaatgattcc
     9841 ttttttccct ttcagaagat tgtatgccat attttttatt tttaaaaata catgcaaaga
     9901 tacggcttta caccaatctt catccgcgtc tggaaaagat aaaagataaa aggaaaaagc
     9961 tctgtcctga gcttgccgaa ggaataaaag tattcgtgat taaccgcgtt tacctgtctg
    10021 caattttaac tgcaacaggc agtcatgcag atccacgtct aacccagcgc tctcgtgttc
    10081 aactttgaaa cgccactgaa tcagtcttca aaataggtga aggctgattc tgcttggccg
    10141 cccatcacgt cacgccagtg actgatgcag gcggattcgc gccaataaga cctgctgagg
    10201 gaaaaaaatt gcttcgcgta aacctgtctg caacaggcag attcgtgaaa ttcgcgtaga
    10261 aaatagtgga cctgcagcgg cgttcacctg tctgcaattt aactgcaaca gtcagtcagg
    10321 tccgcgtcta acaatacaga gaaaattcca ccaaattgta aaagcttatt tccccgtaaa
    10381 cagcccgctt ttgcacagtc cccatttgtg aaagcgatac tgaaaggctg ttccgagcac
    10441 gccgaatccg tatttcatcg agcggcggaa attaatcgaa gaggcttcgt cgaagtattt
    10501 ggtcggacag gtgacttcgg caatatcgaa cccggccata aatatttgtg caatcatttg
    10561 attgtcgaaa acaaaatcgt cggaattggc gtggtaatta attttccgga gcacatcggc
    10621 cgaaaaggtg cggtaaccgg tatggtattc cgataatttc tggccgataa tgaggttttc
    10681 ggtaagtgtg agaatgcggt tgaaaatgta tttgtacaac ggcatgccgc ctttgcgcgc
    10741 accacgtccg agaatgcgtg aaccaaaaac aaccggatac agtccttcgg caataatatg
    10801 caccatcggc gataccagct taggggtgta ctggtaatcg ggatgcagca tcactactat
    10861 atcagcaccc agttccagcg ctttgtcgta gcaggttttc tgattgccgc catagccttt
    10921 attggtttcg tgccggataa tatgccggat acccagtctg gcagccactt ccgatgtgcg
    10981 gtcacggctg cagtcgtcca ccagaataac atcatcaaca atatcgaaag gaatttccga
    11041 ataggtgagt tctattgttt tttcggcgtt gtaggccggg aaaaccacta cgactttttt
    11101 acccgatatc atgaatcaga attaagagat gagtcaggac aaaaatataa aattatttca
    11161 tagtggattt tggcataaaa aatgtaatgc aatcagaaaa ttttcagcca tgaacgcttt
    11221 aaaagaaaaa atgatctggg tcttgtattt ttttctcatc gccggactcg tactggcagg
    11281 aacgataaca atttaaagcc ccatagcccg caagggcacc attcatgact cagtgtaaaa
    11341 gcttccggaa acggaagctt ttttttgatt ttagaaatgc gatttgagat atttgatttt
    11401 tgatgtaata agcattgctg agcgaggtca aggataaggt caaggttgag cacctgcggt
    11461 gagcggtgat cggcgagcag tgattagtga gttttgctga aaacgcaaag aagctttttt
    11521 tacattctgc catcattttt aatttcgaag ttccttgttc agtgttcaga gttcatttgg
    11581 tcaaggataa gcttctatca gttgtcagca atcagctctg agaagaagct ttggagtaca
    11641 acctctcagc gtcgcgcagc gaccatttgc gatagctatc gcaagcgttg aacttgctct
    11701 gtttctggtt gcattgtgaa atgagcgttg cgccctattc cgcaagctct atgccgactg
    11761 ccacatgcgt ttacgtcacc tttataactt tataacatta taact
//
LOCUS       DAOPKE010000084        11791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5712_length_11791_cov_12.168286, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000084 DAOPKE010000000
VERSION     DAOPKE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_11791_cov_12.168286"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            977..1249
                     /locus_tag="PKY63_10005"
     CDS             977..1249
                     /locus_tag="PKY63_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF00989.1"
                     /translation="MKNFIFNNGIGTASISFADKADNVYLLSIKDRKGKLVFNKAVVT
                     EEKNTAQIKVPTKNIKPGLYSLIIRSAEQVYKARIFLGNVCGKLRH"
     gene            complement(1775..3037)
                     /gene="nqrF"
                     /locus_tag="PKY63_10010"
     CDS             complement(1775..3037)
                     /gene="nqrF"
                     /locus_tag="PKY63_10010"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPF00990.1"
                     /translation="MILIDISMGTIIAAGVVVFLFVILLLVGMLLYARKKLMPQGKVT
                     LTINDDQKIEVMPGSSVLSTLSTNGIFLPSACGGGGTCGMCKCQIPDGGGSILPTEVG
                     FFTRKQIKEHWRLGCQVKVRNDMDIHIAPEIFGVKKWECEVISNRNVATFIKEFVLKI
                     PHDERFVFRSGGYVQVYVPGGIEVAFKDFDVEEEYRDEWDKFKMWDLKMKNPEQMYKA
                     YSVANHPALDNIIMLNVRIATPPWDRKSNSFKKVNPGICSSYVFSLKPGDKVTVSGPY
                     GEFHIKETKREMMFIGGGAGMAPMRSHIFDQFETHKTDRKATFWYGGRSLRELFYVED
                     FKRIEDLHPNFKFNIALSEPKPEDNWTGYTGFIHQVILENYLNKHPEPEEIEYYLCGP
                     PQMNDAVQAMLDSLGVPKENIAFDDFGG"
     gene            complement(3053..3670)
                     /gene="nqrE"
                     /locus_tag="PKY63_10015"
     CDS             complement(3053..3670)
                     /gene="nqrE"
                     /locus_tag="PKY63_10015"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPF00991.1"
                     /translation="MQDLINIFVRSIFIDNMVFAYFLGMCSYLAVSKTVKTSMGLGIA
                     VVFVLVITVPVNFLIDSYFLRKGAMVWISDSFAGLDLSFLSFIMFIAVIAAMVQLVEM
                     IVEKFSPALYSALGIFLPLIAVNCAILGASLFMQERELESVGESLAFGLGSGIGWLLA
                     IVAIAAIREKLSYSNVPAPLRGLGITFIITGLMGIAFMSFFGITI"
     gene            complement(3683..4339)
                     /locus_tag="PKY63_10020"
     CDS             complement(3683..4339)
                     /locus_tag="PKY63_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPF00992.1"
                     /translation="MSNGNTNRESAFSAKNLRLLTAPLSKENPITVQVLGICSALAVT
                     VKLKPAIVMALAVTIVIALSNLITSLLRKTIPPRIRIIVQLVIISLLVILVDQVLKAF
                     VYDVSKQLSVFVGLIITNCIVMGRLEAFAMANRPWQSLIDGIGNGLGYGIILIIVSFF
                     REFLGSGTVYGYQIIPESFYAFGYEDNGLMILPPMALIIVGIIIWVHRSSNKDLIDKS
                     "
     gene            complement(4332..5078)
                     /gene="nqrC"
                     /locus_tag="PKY63_10025"
     CDS             complement(4332..5078)
                     /gene="nqrC"
                     /locus_tag="PKY63_10025"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPF00993.1"
                     /translation="MFSNRYIFIYSTIMVVVVAVLLALAATLLQPFQDKNIRVERMQY
                     ILTAINVESTVDNAEELYEKHVTEELVISVDGEIMGRFKDGKMEQGSRRAFDIDLRTE
                     LKKIKDGGAVELPVFIAVNGKDTLTIIPLTGKGLWGPIWGYISLKSDFTTIFGSVFDH
                     KGETPGLGAEIALPEFQQQFVGKKILDENGQFVSVQVIKGGVKNSKIPEIHGVDAISG
                     GTITSNGASMMIESSLKMYVPFISKKISHE"
     gene            complement(5087..6265)
                     /locus_tag="PKY63_10030"
     CDS             complement(5087..6265)
                     /locus_tag="PKY63_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPF00994.1"
                     /translation="MKKGLRKFLDKIKPSFQKGKKLYFLHSTFDAFETFMFVPDKVTE
                     KGAHIRDAIDLKRTMTVVIVALLPALVFGMFNVGVQHFLLKKEFIGMSWQEVISAAGI
                     WPLFWYGFLKVLPIIIVSYVVGLGIEFMFAQIKGHEVNEGFLVTGMLIPLIVPPTTPL
                     WMLAVAVAFAVVFGKEVFGGTGMNIMNPALLARAFLFFAYPSHMSGDAPWSVAADAVS
                     GPTPLAQAASYNVDQMPSLMDSFLGFIPGSIGETSVAMVLIGALILIFTGIGSLRIML
                     SVIAGGVLMSFIMNAWGANAYMEMPPLWHLLVGGFAFGTVFMATDPVTAAQTQWGKII
                     YGLLIGMFTILLRVLNPAYPEGMMLAILMMNVFAPLIDHYVVETNIRRRLRRVKQEIK
                     "
     gene            complement(6278..7630)
                     /locus_tag="PKY63_10035"
     CDS             complement(6278..7630)
                     /locus_tag="PKY63_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPF00995.1"
                     /translation="MRKIYKIRKGLDINLKGEVKTDDIRSHVPDLFAVEPARFRLMKP
                     RLLVQEGDLVECGTPLIQNKLDERMFLTSPVTGLVKRVIRGDKRKLLSVEIEADKKES
                     KINFGKSDPSLIDREAMIAKLLKSGLWPLLRQRPYAIIANPDDTPRDIFISAFDSAPL
                     AVSDHAFIQKNSESFQVGIDVLKKLTPGKVYIGLHAEKTDELVYAPFKNVELRLFSGP
                     HPSGNVGVQIHHIAPVNKGETVWYLYPQDVVAIGKLFLTGYREITRFVSVAGSELKDT
                     YMVEMPAESSLEWLLAGNIKEGDVRVISGNALSGTHVGKQGFLGFYETMLTVIPEGKY
                     FDFFGWAAPGLNKHSASRAFPAFLTPKRKFRLDTNFHGGERAFVLSDQYDKVFPFDIL
                     PVYLLKAILAGDIDKMEKLGIYEVAEEDFALCEYVCTSKIESQQIVRDGIDLMIKEMS
                     "
     gene            7770..9128
                     /locus_tag="PKY63_10040"
     CDS             7770..9128
                     /locus_tag="PKY63_10040"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPF00996.1"
                     /translation="MKKILIYIMLLTGLFSCRTTNPVSGTVSNTDSTSAKHETINENA
                     DDYYQPSRIKYTDEVYVQSIKSVQLHSSEWVFGPPIIWLGKQNQLTLSFDELTDNKKD
                     YYFTFILCDAMWNFVDLPQMDYIDGFFEDPLTSFQFSVNTLINYVHYSVNFPSENMKP
                     KKSGNYILQVYYYENNEKKTVLTKRFFVAENLVGITSDIKMATNIELRNYQHEIDFTI
                     NKSGTEIVDPYRNLTVILQQNGRTDNLITDLKPKLVKGDELEYNYENGNIFDANNEFR
                     HFDIKSITYNTDRIGSILKKDGVFHVYLKTDFRRPFERYLSEEDINGRLLIKNDDGKD
                     SNLESEYVWVHFLLSYEAPLTEGSVFLQGAFTQWGYNEQFKLNYDYKLKGYADSLLLK
                     QGYYNYQYVYLKDVETAADAAYIEGRHAEADNDYTIYVYYREPGELFDRLIGWQTVNS
                     RK"
     gene            complement(9191..10123)
                     /locus_tag="PKY63_10045"
     CDS             complement(9191..10123)
                     /locus_tag="PKY63_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF00997.1"
                     /translation="MKLASTLVLSLFFLIANSQTADEYYEKAVNSVNSNHYSVGLRLI
                     DKAISLDADKADYYIIKAVCYHNLEKYDEALETYDAAIQLFPDSAFLYSLRGVQLQSM
                     RIFDKALANHNIAIKLAKDDSLSIMCYVNRSACYMATRNFKMAYNDLMSAYAIDSMNL
                     NVLNNLGTVCDELGKDDDVFKYLFKIIEIDSTYYGAYINVGFKYQQMGLYDKSLVYFD
                     KAIVYEDDEPLLYSNRSYSRLMTGDLKGAMQDIEKSIKMYPGNSYAYRNRALIYIAQE
                     KFEEACEDLQTALNNGFTEMYGDEVLTLQSKHCK"
     gene            complement(10637..11686)
                     /locus_tag="PKY63_10050"
     CDS             complement(10637..11686)
                     /locus_tag="PKY63_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPF00998.1"
                     /translation="MKKTIVTFVLILAAWMMQAQDFHFSMFNENPLNISPALTGAFPG
                     DHRFVMNYRNQWKSVSEPFITYSGSYDAGLFKKNAESGYLSAGLQFLSDVAGTSQFGT
                     MKVDASVAYHVTLSPSSTLSAGVSGGFCQRSMDETNLYWDNQYDGIGGFNMTVPSGET
                     SMFESFSYGDFSAGLMYSYASTETSMASNDGIKFNIGGAAYHINTPKLNFSAADVEEL
                     FAKYVVHMRGQFGIFGSNTGLVIQGLFANQGPTREIVAGMGLRFMLTEQSHYTGFMKE
                     SALTFGASYRLGDAVIPHVLFEISNFAVGFGYDVNVSGLNVASDGRGGFELFLRYINP
                     SPFRYQNKNFNKSFF"
BASE COUNT         3843 a         2697 c         2106 g         3145 t
ORIGIN      
        1 aaatgacacc gaaacgattg atatatcggg gcaatcattt tcggaagact ccggaggagt
       61 cacatatctg tagtttatga atatacaaac cacgactccg gaggagtcga atataaagag
      121 ttttgtcttc tgattatgcc agtatatacg agctatgtgt tgctagtccg ttttaaaaat
      181 acaaagcata ttacgctatt catactgtga aaaatgaccg gtataaagaa ggataatcaa
      241 aacacattgt tctctggaaa ttttatgaaa cggccgatga aaaaagaaaa tccaaaaata
      301 tggcgaagac aaaaaacatt tgctggccac gaaaaacaat accgggactt ttgtcactcg
      361 aatgcagtga aaacatcacg caaaacgatg cctgttgata tattgcgctg tattgtgctt
      421 tttgacttca aagaatcatt gcggaaatac gatagtctat cgataaaaaa tattttccgg
      481 aaaattttaa cataaaccac catttacaac aaaatccacg tttttggttt gtaaataata
      541 cctgcaatat cataaatccg acttaagcga attcttaaat tgtttacaaa gttaaatata
      601 aaaacatcaa ttataatgtc taaaaggcat atttaacaaa tataaccatt tcgcatattt
      661 acagaaaaca tgcggtaacc ataacaacaa ccccaaaaaa atgatggaaa aacaacccac
      721 tcagtttatg ggtttagcaa aacacacaac aatatttcac tttttttcgg cgcaaaaaag
      781 cattaattta gaattgttaa aatcaggtac attaaaccac tatataaaac aagatatgtg
      841 agcgggctct gttttgactt cataggcaac aaattataag gagcggcaaa gtagggcaac
      901 aatcccgaac tgtcgctccg gttttcggag ggaagttttt atacttcgat acttatttaa
      961 cctaaaaatc attactatga aaaactttat atttaataac ggaatcggca ctgcatcaat
     1021 ttcgtttgca gacaaggcag acaatgttta tctcttgtca ataaaagacc gaaaaggaaa
     1081 actggttttt aataaagctg tggttactga agaaaagaat acagcacaga taaaagttcc
     1141 gacaaaaaat ataaaaccgg gtttatactc attgattatc cgttcagcag agcaggttta
     1201 caaagcacgg atttttctcg gaaatgtctg cggcaaacta agacattgaa tttataaagc
     1261 ataaagtcat cagcaatcag ctttgaggcg gggctgagga gaaggtgcgt aacatgtggt
     1321 ctgtggcgca atgcacaacg catggagcaa gagcatggct tagtatcgct cgcgcgcaga
     1381 agtcactctg aaccccgcct gctgtcattc agagccctgc gttccgcagg ataaactccg
     1441 tcgaagagtt gacaaaagga tagactccgt ctgaatgtct atagtttggg atactccgtc
     1501 ggagaggcgc tctgaataag acatgcgtct ctgcgttctc cgggcggttt aatctgaatc
     1561 gggcttcatt gcaggcctgc ctgttgcaga ttcgcggaag aaagattgga ttagcttcga
     1621 aggttttcaa ggagatcccg gctctccaca agctgcacca ttcgataagc tggcgccagc
     1681 tccggccggg aactcagtgg tgtaatgatt tttgtgcctt cgtggtaaaa tttccctgcg
     1741 cctctgcgag ctctgcgcga aaccctctgc gggcttatcc accaaagtcg tcaaaagcaa
     1801 tattctcttt aggcactcca agactatcga gcatggcctg caccgcgtcg ttcatctgcg
     1861 gaggtccaca caaataatat tcaatttctt caggctccgg atgcttattc agataatttt
     1921 cgagaatcac ctgatgaatg aagcccgtat agccagtcca attatcctcg ggttttggct
     1981 ctgacagagc aatattgaac ttaaaattgg gatgaagatc ctcaatccgc ttaaaatctt
     2041 caacataaaa aagctcacgc aaagaacgtc caccatacca aaaagtagct tttcgatcgg
     2101 ttttatgcgt ttcgaactga tcaaagatat gagaacgcat aggtgccata ccggctccac
     2161 caccaataaa catcatttcc ctctttgttt ccttaatatg gaattcgcca taagggccgc
     2221 ttacagtaac cttatcgcca ggtttcaaag aaaacacata gctggaacaa atacccggat
     2281 taactttttt gaaactgttg gatttgcggt cccagggcgg tgttgcgatg cgcacattga
     2341 gcatgataat attatccagc gcagggtgat tggcaacaga ataggcctta tacatctgct
     2401 ccggattttt cattttcaaa tcccacatct taaacttatc ccattcatcc ctgtattcct
     2461 cttcaacatc aaaatcctta aatgccactt ctattccgcc gggcacatat acctgaacat
     2521 agcccccact gcgaaaaaca aaacgttcat catgtggaat tttcaaaaca aattctttaa
     2581 tgaatgtagc aacatttctg ttggaaataa cctcgcattc ccattttttc acaccgaaaa
     2641 tttcaggagc aatatgaata tccatatcgt tgcgcacttt cacctgacaa cccagccgcc
     2701 aatgttcctt gatttgtttt cgtgtaaaaa acccaacttc ggtcggcagg attgaacctc
     2761 cgccatcagg aatctgacat ttgcacatac cacaggttcc acctccaccg caagcagaag
     2821 gaagaaaaat accattggta ctcaaagtag ataaaacact ggaccccggc atcacctcga
     2881 ttttctggtc gtcgttgatg gtgagagtta ccttaccctg tggcatcagt tttttgcgtg
     2941 cgtaaagcaa cataccaacc aacagcagta ttacaaataa aaaaacaaca acacccgctg
     3001 ctatgattgt tcccattgat atatcgataa gtatcatacc cctatttttg aattaaattg
     3061 taatgccgaa aaaactcatg aaagcaatac ccatcagtcc tgtaatgata aaggtaatgc
     3121 ccaatccacg caaaggagcc ggaacattgg aataggaaag tttttcgcgg atggcggcaa
     3181 tggcaacaat ggccagcaac caaccaatac cagatccaag accaaaagcc agggattctc
     3241 cgactgattc aagttcgcgt tcctgcatga aaagcgatgc tcccagtatt gcgcagttta
     3301 cagcaattaa cggaagaaaa attccaagcg cactatacaa agcagggctg aatttttcca
     3361 caatcatttc caccaactgc accatagccg caataaccgc aatgaacata ataaaactca
     3421 gaaaactcag atccagtccg gcaaacgaat cgctgatcca gaccatagcg cccttgcgaa
     3481 gaaaataact atcgattaga aaattgacag gcactgtaat caccagcaca aaaacaactg
     3541 caattcctaa ccccatggat gtttttacag ttttcgatac agccagatag gagcacatgc
     3601 ctaaaaaata ggcaaacacc atattgtcga taaaaatgct cctgacaaat atatttatta
     3661 aatcctgcat agtttttcaa ttttagcttt tatcaatcaa atccttgttg ctgctgcgat
     3721 gaacccagat aattatacca acgataatca gagccatggg cggtaaaatc atcaatccgt
     3781 tatcttcata accgaaggca taaaaacttt ctggaataat ctgataacca tacacagttc
     3841 ctgaaccaag aaattcgcgg aaaaacgaaa caataatcag aataatacca tatcccaaac
     3901 cattccctat cccgtctatc aatgattgcc agggtcgatt ggccatggca aaggcctcaa
     3961 gacgccccat gacaatacaa ttggtaataa tgagccccac aaaaaccgac agttgcttgc
     4021 ttacatcata aacaaaagct ttcagcacct gatccaccaa aattaccagc aaagaaataa
     4081 tgaccagttg cacaataatg cggattcggg gcgggatggt tttgcggagc aacgaagtta
     4141 taagattcga tagtgcaatc acaatggtaa ctgccaaagc catcactatg gccggcttaa
     4201 gttttaccgt cactgcaagt gccgagcaaa taccaagcac ctgaaccgta atcggatttt
     4261 ccttactcag cggtgcagta agcaacctca gatttttggc cgagaacgca gattctctgt
     4321 tagtgtttcc gttactcatg acttattttt tttgaaataa aggggacata cattttcaaa
     4381 ctgctttcaa tcatcatcga tgcaccgtta ctggtaatgg ttcctccgga aatagcatca
     4441 acaccatgaa tttccggaat cttactattt ttcacgccac ctttgataac ctgcacactc
     4501 acaaactgtc cgttttcatc aaggattttt tttccgacaa attgctgttg aaactcaggt
     4561 aaggcaatct cagcgccaag tcctggagtt tcgcccttat ggtcgaatac tgaaccgaaa
     4621 atggtagtaa aatcgctctt taagctgatg taaccccaaa tgggccccca gagccctttc
     4681 cctgtcaatg gaattatagt cagcgtatct ttcccgttta cagcaataaa caccggaagc
     4741 tcaacggcac caccatcttt gattttttta agctcggtcc gcaaatcaat atcaaaagca
     4801 cgacgactac cctgctccat ttttccatct ttaaaacgac ccatgatttc accatcaacg
     4861 ctgataacaa gttcttcagt aacatgtttt tcatacaact cttcggcatt gtcaaccgta
     4921 ctctctacat tgattgctgt gagaatatat tgcattcttt caacacggat atttttatcc
     4981 tgaaagggct gaagcagcgt tgccgccagt gccagcaata cagccaccac aacgaccata
     5041 atggtggaat aaataaatat gtatctgttg ctaaacataa gccctcctat ttaatttcct
     5101 gttttaccct tcgcaaacga cgacggatat tggtttcaac aacataatga tcaatcaatg
     5161 gtgcaaaaac attcatcatc agaatagcca gcatcatacc ttccggataa gccggattga
     5221 ggacccgaag caaaatggta aacattccga tgagcaatcc ataaattatt tttccccact
     5281 gagtctgagc tgcagtaacc ggatcggtag ccataaacac cgttccgaaa gcaaatccac
     5341 ccaccaataa atgccacaaa ggaggcattt ccatgtaagc attggcgccc catgcattca
     5401 tgatgaaaga catcagaaca ccacccgcaa tcacactcag cataatccgc aaactgccga
     5461 tacctgtgaa gataagaatc aatgcgccaa ttaaaaccat agcaaccgac gtttcaccga
     5521 tagaacccgg aataaaacca agaaatgaat ccatcagcga aggcatctga tcgacattgt
     5581 acgatgctgc ctgagccagc ggagtcggac cactgactgc atcggcggca acactccatg
     5641 gagcatcgcc cgacatatgc gaaggatagg caaaaaacaa aaatgcgcgc gccagcaaag
     5701 ccggattcat aatgttcata cctgttcctc caaatacttc cttaccaaac actacggcaa
     5761 aagctacggc tacagccagc atccagagcg gagttgtggg aggaacaatt aatggtatca
     5821 gcattccggt taccagaaat ccttcattta cttcatgtcc tttgatttgc gcaaacataa
     5881 attcaatgcc caatccaaca acataagata caattataat gggcaacact ttaagaaaac
     5941 cataccaaaa caaaggccag atgccggctg cagaaatcac ttcctgccaa ctcatgccta
     6001 tgaattcttt tttcagcaaa aaatgctgca ccccaacatt gaacatgcca aaaaccaatg
     6061 ccggaagcag cgcaacaata acaaccgtca ttgtacgctt caggtctatg gcatcgcgga
     6121 tatgtgcgcc tttttcagta accttgtcag gcacaaacat aaatgtttca aacgcatcaa
     6181 aagtagaatg aagaaaataa agtttcttcc ctttctgaaa cgatggtttg attttgtcta
     6241 aaaattttct gagtcctttc ttcatagaaa tcatgtatta actcatttcc ttaatcatca
     6301 gatcaatgcc gtcgcgtaca atttgctgtg actcgatttt tgaagtgcaa acatattcac
     6361 ataatgcaaa atcctcttct gccacttcgt aaatgcccag cttttccatt ttgtcaatat
     6421 caccggccaa tatagctttg agcagataca ccggcaatat atcgaacgga aaaaccttat
     6481 cgtattgatc actcaaaaca aatgcgcgtt caccaccatg aaaatttgta tccagtctga
     6541 acttacgctt cggtgttaaa aaagcaggaa acgcacgaga agcactgtgt ttattcaagc
     6601 caggtgcagc ccaaccaaaa aaatcaaaat attttccttc tggaataacc gtcagcattg
     6661 tttcataaaa ccctaagaaa ccctgcttcc caacatgggt tcctgaaagc gcattgccac
     6721 tgataacacg tacatcacct tctttgatat taccggcaag caaccattcg agcgaagact
     6781 cagccggcat ttcaaccata tacgtatctt tcagttcact gccggcaaca gatacaaaac
     6841 gtgtaatttc acgatatccg gtaagaaaca atttgcctat tgccacaacg tcctgcggat
     6901 ataaatacca aaccgtctcg cctttattga caggagcaat atgatgaatc tgaacaccaa
     6961 cattaccgga aggatgcggc ccgctaaaaa gacggagctc cacatttttg aatggtgcat
     7021 ataccaattc atcggttttt tctgcatgca atccgatata gactttgcca ggagtcagct
     7081 ttttcaaaac atcaattccg acctgaaatg attctgaatt tttctgaata aaagcatgat
     7141 cggaaacagc cagcggagcc gaatcaaagg ccgaaataaa aatgtcgcgc ggggtatcat
     7201 cagggtttgc aataatggca tatggtcgct gacgaagcaa aggccaaagc cccgatttca
     7261 gcaattttgc aatcatggct tcgcgatcaa tcagcgatgg gtcgcttttg ccaaaattta
     7321 tttttgattc ttttttgtct gcttctattt ctacagaaag aagttttcgc ttgtcacccc
     7381 gaatgacccg cttcacaagc cctgtaacag gcgaagtcag aaacattctt tcatcaagtt
     7441 tgttttgaat cagtggcgtt ccacattcta ccaaatcgcc ctcctgtacc agcaatcgcg
     7501 gtttcatcag acgaaaacga gcgggctcta cggcaaaaag atccggaaca tgactgcgga
     7561 tatcatcagt ctttacttct cctttcagat taatatcaag ccctttgcgg attttataaa
     7621 tctttcgcat atgagataga aatttcagca aatttaaaaa ttaatcgtta cggaacaaca
     7681 acgcgataaa tcgcatcaac acatatttac aacatcttga tatttcacca atgcgattat
     7741 cgctatattt gcgtgtgaat tgcgaatata tgaaaaaaat actgatatat atcatgttgt
     7801 tgacaggcct tttttcctgc cgcacaacaa accctgtgtc cggcacagta agcaatactg
     7861 acagcacttc tgccaagcac gaaaccatca atgaaaatgc ggacgattat tatcagccat
     7921 cacggataaa atataccgac gaagtgtatg tgcaaagcat caaatcagtc caattgcatt
     7981 catctgaatg ggtatttggt ccgcccatca tatggctcgg aaaacaaaac cagctcacac
     8041 tgtcgtttga tgaactaacc gataacaaaa aagattatta ttttacgttt attctatgtg
     8101 atgccatgtg gaactttgtc gatttacctc aaatggacta catcgatgga ttcttcgaag
     8161 atccgcttac ttcttttcag ttttcagtca atacgctgat caattacgtg cattattctg
     8221 tcaattttcc ttccgaaaac atgaagccga aaaaaagcgg aaactatatt cttcaggttt
     8281 attattatga aaacaacgaa aagaaaactg tgctaactaa acgttttttt gttgctgaaa
     8341 accttgtagg aatcacgtcc gatataaaaa tggcaacaaa tatcgaatta cgcaattacc
     8401 agcatgaaat cgattttacc atcaacaaaa gcggaactga aattgttgat ccataccgca
     8461 atctaacggt tattctgcag caaaatggca gaacagataa tctgataaca gatctgaaac
     8521 caaaacttgt gaaaggcgat gaacttgaat acaactacga aaacggaaat atttttgatg
     8581 caaacaacga atttcgtcat tttgacatca aaagcattac atacaacacc gaccgcatcg
     8641 gaagcatcct taagaaagac ggagtatttc acgtatatct gaaaacagac ttccgccgac
     8701 ctttcgaacg atatctttct gaagaagaca tcaatggacg actgctgata aaaaatgatg
     8761 acgggaaaga cagcaacctt gaatcggaat atgtatgggt gcattttctg ctgagctacg
     8821 aagcgcctct gaccgaagga tctgtttttt tgcagggcgc ttttactcaa tggggataca
     8881 atgaacagtt taaactgaat tacgactaca agctgaaagg ttatgccgat tcactgctgc
     8941 tgaaacaagg ttattacaat taccaatatg tatatctcaa agatgtggaa accgcagccg
     9001 atgcggccta catcgaagga cgccatgcag aggccgacaa tgattacacc atctatgttt
     9061 attaccgcga acccggagaa ttgttcgatc gtttgattgg atggcaaact gtgaattcga
     9121 gaaaataatc taaaaatgat ggataagaaa taacagagga gttattcgta atcagctgaa
     9181 caatcacaaa tcacttgcag tgtttgcttt gcagagtcag gacttcgtcg ccatacattt
     9241 ccgtaaagcc attattcaac gccgtctgaa gatcctcgca ggcctcttca aatttctcct
     9301 gagcaatata aatcaaagcc ctgtttcggt aggcatatga atttccggga tacattttga
     9361 ttgatttctc aatatcctgc atggcgcctt tcaggtcgcc tgtcatcaaa cgactataac
     9421 ttctgttgct gtataaaagc ggttcgtcat cttcatacac tattgcttta tcaaaataca
     9481 caagggactt gtcatacaaa cccatctgct ggtatttgaa cccaacatta atataggcgc
     9541 cataatacgt tgaatcaatt tcaataattt tgaacagata tttgaataca tcatcatctt
     9601 taccaagctc atcacaaaca gtacccagat tattcaatac attcagattc attgaatcaa
     9661 tggcataagc actcattaaa tcattataag ccattttgaa attccgggtt gccatataac
     9721 atgcagatcg gttcacataa cacattatgc ttagcgaatc gtccttggcc agtttaatgg
     9781 caatattatg attggccagc gctttgtcaa atatcctcat cgattgcaac tgaacacctc
     9841 ttaaactgta aagaaatgcc gaatcaggaa acagctgtat ggcagcatca taagtttcca
     9901 atgcctcgtc atatttctca aggttgtgat agcaaacggc cttgataata taataatctg
     9961 ctttatccgc atcgagactg attgctttat caattaaacg caatccgacc gaataatgat
    10021 ttgaattgac cgaatttaca gctttttcat agtattcatc agctgtctga gaattagcta
    10081 tcagaaaaaa caatgataaa accaatgttg aagcaagttt cataatattc tgatttttat
    10141 tatgcaaaaa taaaatatat tttaaatagt cgttttggtt ttacacaact tattctttgt
    10201 acaaaaaatc gttgattaca agtcatgcca aatggaaata caaaatagtc aattgtagca
    10261 atttcattga acgttttgtt gggtgatgcc tgacaaaaca ggcaggcatc gcatatctgt
    10321 agaaaaacca ataccgacca cacgacctca gagaggtcgc atattaacaa gcattgtcag
    10381 aagacgaagc ttcttatatg cgactcctcc ggagtcgtgg ttttgtttta tatggtctac
    10441 agatatatga ctcctccgga gtctcccaaa aataattgaa ccgatttata aatcactttg
    10501 gtatcagata aaccgcttaa agggaattgc aacagctcat tcatcaagaa agccacaaca
    10561 acaaaaccgg ttttccactg ctgcgcatga cagacaatga aaaaccggtt taaaaaaaac
    10621 aaatgaactc gtttttttag aagaaactct tattgaaatt cttattctga taacggaatg
    10681 gactcgggtt gatgtagcga aggaacaatt cgaagccacc acgtccatcc gaagccacat
    10741 tcaaaccaga cacgtttaca tcgtatccga aaccaacagc aaaatttgaa atttcaaaca
    10801 acacatgagg aatcactgca tctcccaatc ggtacgaagc tccaaaagtc agcgcagatt
    10861 ctttcataaa gccagtataa tgcgattgct ctgtcaacat aaaacgcaga cccataccgg
    10921 caacaatttc acgtgttggt ccctgattgg caaacaaacc ttgaattacc aaaccagtat
    10981 ttgacccaaa aatgccaaac tgtccacgca tatgaaccac atatttggca aacaattctt
    11041 caacatcagc cgctgaaaaa ttcagcttcg gtgtgttgat atgataggca gcgccaccaa
    11101 tattaaattt gattccatca ttcgaggcca tggatgtttc ggtcgaagca taactataca
    11161 tcaaaccagc cgaaaaatca ccgtaactaa acgattcaaa catgctggtc tctccgcttg
    11221 gcacagtcat gttgaacccg ccaataccat catactgatt atcccaatac agatttgttt
    11281 catccatact acgctgacaa aacccgccac tgacccctgc actcaacgta ctagaaggac
    11341 tgagcgttac atgataagct accgatgcat caaccttcat ggttccaaac tgactggtgc
    11401 ctgcaacatc actcaaaaac tgcaacccag ccgacaaata accggattct gcatttttct
    11461 tgaataaacc ggcatcatat gaaccggaat aagtaataaa cggctccgat acactcttcc
    11521 attgattgcg ataattcatc acaaaacgat gatcacccgg aaaagcacct gtcaatgcag
    11581 gactaatgtt caaggggttt tcattgaaca tggaaaaatg aaaatcctgc gcctgcatca
    11641 tccatgcagc cagaatcaat acaaatgtta caattgtctt tttcatattt tgttgttttt
    11701 ctttttgcaa tttattatct ggtcaatgtt acatctcctt tttcaatctt atccgaacca
    11761 tccataaata tggcatgcag ataatacgta a
//
LOCUS       DAOPKE010000085        11638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5805_length_11638_cov_11.895019, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000085 DAOPKE010000000
VERSION     DAOPKE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_11638_cov_11.895019"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2294)
                     /locus_tag="PKY63_10055"
     CDS             complement(<1..2294)
                     /locus_tag="PKY63_10055"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPF00999.1"
                     /translation="MRLLIVAICLLFSGFAIAQTSDLGKTEPTMLENKGQVSNSDGTP
                     ANHVLFYMNTPQLDFYLTKTGISYVFKKIEIIPEEGDGRTDPKHRQEKTTVYSHRVDV
                     TAIGAKISPDKAEVEYSDDRTVSIYNGLVPEGLENMKPVRTVTLKNVYPGIDWQFVVQ
                     EENIKYNFILHEGADLNSIRLRFDGQNKLSIENDRLIINTAYGTLTEEPLVSFLSTGE
                     SVNIKQNLQENLLSFTAENLPALQNGQTLTIDPPLVWATFFGGTGSERVLVVERSLDG
                     FVYMLLEVLSTDFPVLDSASLPWYQSISGGTTDVGILKFNDGGKLVWSSYYGGSGNDK
                     PYNMFCDNNFIAIVGSTTSPVFPTQPFVSAYYQSTLKGTQDAFVVVFGKTSPVVDQRI
                     WGTLLGGYSNYDVASDCSFDNGKLYVVGHTVNDSAANWNKPNSVVQFPIVPKAGAYNQ
                     NAILGSGDKYELFIMEFGLGFLMNWSTFYGGSGNESEPSCDIDSIGRLGIAFNSTSSD
                     IPVMSTMTGSLARPYSVVTDAAFSLFDQNGQKMTHTYLGGILDDYAEDLICNKHNEWL
                     ITGSTRSTNFPVANNIGAGLYFPTLTGTYNAFLLKLDSTIALDYSTYYGSGTATGYGL
                     TIDSHDNLYLVGSTKASMFTYNPLDGSYVDSTINSTAVHDGFLLELDSNYNPKWATVI
                     GGSGDDPLYDIRVSPNDHVFVAGYSQSPNQPLQNPGGGAWFDNVLSGTREAIIMKFVP
                     CPENFDTIWAVDSVCYGETIDIYAT"
     gene            2823..3911
                     /locus_tag="PKY63_10060"
     CDS             2823..3911
                     /locus_tag="PKY63_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPF01000.1"
                     /translation="MQRYSKTQLFSLVEKIFLKAGCSSDNAKTVANMLVTAELRGIPS
                     HGLMRIKDYIGLWQKGRMNMCPDLKIVHETPSTATVDGDMAPGMLAGKFAMELAIRKA
                     EQVGSGWVAVRNSNHFGIAGFYTMMAAQHNMIGIAMTNANALVAPTFSVDRMLGTNPI
                     AFTIPGKEEPAFTADFATTPIARGKLELLEKQGKESPQGFVQDAEGKAASDPSVLRRG
                     GAILPLGGDYEHASHKGYCMGAMVDILSAILSGANFGPFVPPQVAYLDPKPGAPGAGL
                     GHFFGAMRIDGFRPLDEVKEYMDLWIRTFRQSKPAEGRDRVIIPGEPELAHEKEHLRE
                     GIPVVPQVWQELITTASSLGIAEDDLII"
     gene            4015..4593
                     /locus_tag="PKY63_10065"
     CDS             4015..4593
                     /locus_tag="PKY63_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPF01001.1"
                     /translation="MSEKEELKKENQPNAENPEQNDATLNESESSKVEGDEKQCPDAV
                     VETMESLQLKANEWQDKYTRLFADFENYKKRMRQERFDLLQTAGSELMLDILPVIDDF
                     ERGFASLATATDIEAVKQGYELIYNKLVNILKQKGLEPMESKNEVFDTDLHEAITMIE
                     SPDMKGKVVDVVQKGYKMRGKVLRYAKVVVGN"
     gene            4606..5760
                     /gene="dnaJ"
                     /locus_tag="PKY63_10070"
     CDS             4606..5760
                     /gene="dnaJ"
                     /locus_tag="PKY63_10070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPF01002.1"
                     /translation="MSKRDYYEVLGVSKNASADELKKAYRQMAIKYHPDKNPDDKTAE
                     EKFKEAAEAYEVLSNPEKRQRYDQFGHAGMNGAGGFGGGMSMDDIFSNFGDIFGDFFG
                     GGFSGFGGGFGRSRSSQRVPQGSNLRIRVKLTLEEIANGVEKKVKVTKSVPCESCNGT
                     GARAGSKPTTCTTCHGSGRVMRVTNTFLGQMQTASTCPHCNGEGTIITDRCPSCSGTG
                     LTKGEEVIAIKIPAGVENGMQLSMSGKGNAAPRGGVPGDLIILIEEVEHELFKRDGIN
                     LLYEHNISYADAVLGTQIDVPTLDGKARIKIPAGTIPGKMFRLKGKGLPALNSYGRGD
                     LIVSINIWVPQSVSKDEKTFLEQMNTKNGFQPDNSQKKKSFFDRMKEYFD"
     gene            5770..6708
                     /locus_tag="PKY63_10075"
     CDS             5770..6708
                     /locus_tag="PKY63_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF01003.1"
                     /translation="MIYFEAQEVSKNFANHRALDNVSIQVKEQSIFGLLGPNGAGKTT
                     LIRIINQIIGPDSGRLFIKGHPLDVQHTEDIGYLPEERGLYKKMKVGEQALYLAQLKG
                     ISKSDAYLRLKQWFEKFGIEDWWNKRVEELSKGMQQKVQFIITVIHAPSLLIFDEPFS
                     GFDPINAEMLKEEILQLKEKGATIIFSTHNMASVEELCDDIALINQSKVILSGNVQAI
                     KQKYSSNVYQLKLRAAQMCDVSNFSEMGIVSGFENQSDLCSFRIELNPNVTSNDLLMA
                     SMAFGHVTSFEELMPSMNDIFISKVTGNKIHKKSRK"
     gene            6705..8039
                     /locus_tag="PKY63_10080"
     CDS             6705..8039
                     /locus_tag="PKY63_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF01004.1"
                     /translation="MKKILIILRREYLSRVQKKSFIVMTILGPILMAALFVVPVYLAN
                     VSDQHKKIAVLDETGLFHDKFPNSNKVIFENVYMSYQAASENLEALGYDAVLYIPESV
                     INNPNAVKMSSEKDMGFGIVDLIEGVIKKELEAHRLSLSGIDKKILDNVNVNVKISTY
                     ILREGEEEQSFSEISYILGMLGGILIYTFIFLYGSQVMRGVIEEKTNRIVEVIVSSIK
                     PFQLMLGKIVGVALVGLTQFVLWIGLTAVIVFSLQQLNPDMFKYKEPPKVMVQDKGLS
                     QSDMAQQQEALQLSESRSNQILEGISHIQFGNILIVFVFYFLFGYLLYASMFAAIGSA
                     VDNDADTQQFMLPVTVPLILALVSLNFVLNNPDGPVSFWLSMIPFTSPIVMMARIPFN
                     PPVPLWEIGLSMAFLIAGFILTTWIAGRIYRTGILMYGKKPTVRELFKWMKY"
     gene            8416..9087
                     /locus_tag="PKY63_10085"
     CDS             8416..9087
                     /locus_tag="PKY63_10085"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPF01005.1"
                     /translation="MNIKEYHRRLSDALQAAANPVVAADAKKYMKNKSDFFGVSSPKR
                     KEILTAFLKENHFPAIHDIELFALMCWDSSQREMQYCCMEILYRMRKKLTPAHLPLFE
                     NMIQTKGWWDTVDFIAPSLAGFILKNNPELIDETISRWQHHDNMWMRRSAILHQLKYK
                     TDTDQKRLFSICKALAHEKEFFIRKAIGWALREYSKVNSDAVRSFVATTELSGLSQRE
                     ALKRI"
     gene            9203..9655
                     /locus_tag="PKY63_10090"
     CDS             9203..9655
                     /locus_tag="PKY63_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01006.1"
                     /translation="MKNEKPLIGSIMYEYQASSVAPQYHRSYVLLVSPDKIKVTVDSY
                     GTILTDTVILISINQFNEIVAEYDSLAFRPVPKKDNRGCVGGTGAILKVSDISDTLIF
                     DGYISFCGGQEFGNMEGDVKKLSEKIKSYIPDFELLLKRDWKPEETDE"
     gene            9648..10214
                     /locus_tag="PKY63_10095"
     CDS             9648..10214
                     /locus_tag="PKY63_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF01007.1"
                     /translation="MNDILENLSVFDVNDEDFEHKSYLHGRLHTQRVMAWVCVLAQKL
                     NLSEDGRLAFFAARIHDLGRLTDGREPGHGLRSADYYLPKYRIVFEKYGMRSEDYITV
                     YNAVKWHSRSDEPDKNIKNIDVIKLLKDADGLDRVRLGDDEPDIRFFRNDISKRFVSH
                     ARLLYNITEEKENMSLKEIVELAFELTN"
     gene            complement(10368..11399)
                     /locus_tag="PKY63_10100"
     CDS             complement(10368..11399)
                     /locus_tag="PKY63_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPF01008.1"
                     /translation="MKTKNLIVTLIIVTFVAFSCGGKKTYDENSKSGESEIVSEESAI
                     VKTSAQEDQKSGYAPTSTSETIEKSDAEGKKDDYAAMISSSAAQMSKFDSTHKLIRTA
                     ELKFRALDVTQTAFNIEDIVKKFGGFTASTDLSSKVTGKETTRISPDSLIETTTYHFT
                     NEMVVRVPAKNMDTVLKCLVPMIDFLDYRNIKAEDVSLAYLRKQLIKKRLDLYNIQVS
                     GLTTSGNSGDRLAAMESQLQKQIQNDEALLEKLELDDQVNYATITLHIYGRDKTRHVM
                     LPDEERIDNYKPGFGKKLMQAINSGWQVLQLFVIGLITLWPLWLAGALVWFIIARINK
                     NKKSKQLPK"
BASE COUNT         3296 a         2266 c         2721 g         3355 t
ORIGIN      
        1 gttgcataaa tatcaatggt ctcgccgtag cagacagaat ccactgccca gatggtatcg
       61 aaattttcag ggcaggggac aaatttcatg ataatggctt cgcgcgtacc actgagaaca
      121 ttgtcaaacc aggcgccacc gcccggattc tgaagtggtt gattgggtga ttgagaatag
      181 cccgcaacaa aaacgtggtc attgggcgaa acgcggatat cataaagcgg atcatcgccg
      241 gagccaccaa tcaccgttgc ccatttcgga ttgtagtttg aatcgagttc cagcaggaac
      301 ccgtcatgaa cagcagtact gttaatggtg ctatctacat atgagccatc cagaggattg
      361 tatgtgaaca tactggcttt ggtgcttcca accaaataaa ggttatcgtg tgagtcaatg
      421 gtaaggccgt atccggtggc ggtgccgctg ccataatatg tgctgtaatc cagagcgatt
      481 gttgaatcga gttttaagag gaaggcatta taggtgccgg ttaatgtcgg aaaatagagc
      541 ccagccccga tattgttggc cacagggaaa ttggtcgatc gtgtggaccc cgtgataagc
      601 cattcattat gcttgttgca gataagatct tcagcataat catcaagaat tcctcccaaa
      661 taggtatgcg tcattttctg gccgttctga tcgaacagag aaaaagcagc atcagtaacg
      721 actgaatatg gccttgccaa actccctgtc attgttgaca taaccgggat gtctgaagag
      781 gtagaattaa aagcaatgcc aagtcgtccg atagaatcga tgtcgcacga aggttcactc
      841 tcatttcctg aacctccata aaaggtactc caattcatta aaaagccaag accaaattcc
      901 ataataaaaa gttcatattt atcaccactg cccaaaatag cattttggtt atatgctcct
      961 gctttgggta caatgggaaa ctggacaacg gaatttggct tattccagtt tgctgcacta
     1021 tcgttaacag tatgcccgac aacatacaat ttaccgttat caaacgagca atcactagcc
     1081 acgtcataat tggagtagcc gccaagaaga gtaccccaaa tacgttggtc aacaaccggg
     1141 gatgttttac cgaaaacaac tacaaatgcg tcttgcgtgc ctttcaatgt tgattggtag
     1201 taggcgctta caaacggctg tgttgggaaa accggagatg ttgtgcttcc tacaatagcg
     1261 ataaagttat tgtcgcaaaa catgttgtag ggtttatcat ttccgcttcc tccataatac
     1321 gaagaccaga cgagtttgcc cccatcattg aatttcagaa tgccgacatc ggttgtgccg
     1381 cctgagatgg attggtacca tggcaatgaa gctgaatcaa gtacgggaaa atcggtagaa
     1441 agtacttcaa gcagcatgta cacaaaacca tcgagactac gctctactac cagtacacgt
     1501 tcgctacctg tcccgccaaa gaatgtagcc cacacgagcg gcgggtcaat ggtcagggtt
     1561 tgtccgttct gcagtgcagg caggttctcg gctgtaaatg aaagcaggtt ttcctggaga
     1621 ttttgtttga tattgaccga ttctcctgtg gagagaaacg aaaccagcgg ctcttcggta
     1681 agcgtcccgt atgcggtatt gatgatcagc cggtcatttt caatgctcag cttgttctgc
     1741 ccgtcaaatc tcagtctgat gctattgagg tcggctcctt catgaagaat gaaattgtat
     1801 ttgatgtttt cctcctgcac cacaaactgc cagtcgatgc cggggtacac atttttgagt
     1861 gtaacggtac ggaccggttt catattttcc agtccctccg gtacaaggcc gttgtaaatg
     1921 cttacagtgc ggtcatcgga atattccact tcggctttgt cagggctgat ttttgcgcca
     1981 atggcagtaa catccacgcg gtgactgtac actgtggttt tctcctggcg gtgtttcgga
     2041 tctgtccggc cgtcgccttc ttcaggaatg atttcaattt ttttgaaaac ataggaaatc
     2101 cctgttttgg tcagatagaa atcgagctgt ggcgtgttca tgtaaaacag cacatgattg
     2161 gccggggtgc catctgaatt tgagacttgc cctttgtttt cgagcatggt gggttccgtt
     2221 ttgcccagat cggaagtctg tgcaatggca aatccggaaa aaagcagaca aatagctaca
     2281 atcaataatc gcataatgtt catattacag cgttaagaca attatattaa gagaacgttg
     2341 cagtgcaaag ttagatattt ctgaaaatat catgggaaaa tgagtcattt gaagacaatc
     2401 agccattttt tacagacgac tgcattcgtc cataaatcgc ccacctgaat tggaatcagg
     2461 gatcgtcaaa aaacacaagc ttctttaaga acgaaggttg atgttgaatg cttgaagtca
     2521 gcattggcag catcgaccag aagtattcgc atgattttta atgcgttgag gatgtctcaa
     2581 aatagcggtt tctacaaaaa caatattcca tcgtccttaa gcgacacagg agcggaagga
     2641 tatggaatcg ttgtttttgg gtttgttacg acgattccat attccggccg gagtatcttt
     2701 tttttaattc aaaactggtc ggaacgatgg aatatcgtaa cgattttgta tttttgagac
     2761 agtctcatcc acgattaaat tcgggataag cttcaaaatc gtaattttgc aaaaaaattg
     2821 cgatgcagcg atacagcaaa acacaactgt tttcattggt tgaaaaaata tttctcaaag
     2881 ccggttgttc ttcagataat gccaaaacgg tagccaatat gttggtaacg gctgaattaa
     2941 gagggattcc cagccatgga ttgatgcgga tcaaggatta tatcggtctt tggcagaaag
     3001 ggcgcatgaa catgtgtcct gatttgaaaa tcgtgcatga aaccccttct acagccactg
     3061 ttgatggcga tatggctcca ggtatgctgg ccggaaaatt cgccatggag ctggccatcc
     3121 gaaaggcgga gcaggtgggc tcgggttggg tggctgtgcg caattcaaat cattttggca
     3181 ttgccggttt ttacaccatg atggctgcgc aacacaatat gatcgggata gccatgacaa
     3241 acgccaatgc gctggtggcg cccactttca gtgttgaccg aatgcttggt accaatccga
     3301 ttgcatttac gattccggga aaggaagaac cggctttcac tgccgatttt gcgactacac
     3361 ccattgcccg cggcaagttg gagctattgg aaaaacaggg aaaagaatcg ccgcagggat
     3421 ttgtgcagga tgctgaaggc aaggctgcaa gcgatccttc ggtgttgcgt cgaggtggtg
     3481 ccattctgcc tctgggcggc gattatgagc atgccagtca taagggatat tgtatggggg
     3541 ctatggtcga tattttatca gcaatattgt caggtgccaa ttttggtcct tttgttccgc
     3601 cacaggttgc ttacctcgac ccaaagccgg gcgctccggg agcaggactt ggtcattttt
     3661 tcggcgccat gcgcatcgat ggttttcgtc cgcttgatga agtgaaagaa tatatggatt
     3721 tgtggataag gacattccgg cagtcgaaac cggctgaagg gcgcgatcgt gtaattattc
     3781 ccggcgaacc ggaattggca catgaaaagg aacatttgcg tgagggtatt ccggttgttc
     3841 cgcaggtttg gcaggaattg atcacaacgg cttcatcgct tggtattgca gaagacgatt
     3901 tgattatctg acaaaaaggc acatctggag ccactggcaa actttttgac agccacgcgc
     3961 gcgattgaaa acgatagccc attcaatatg aaatttggaa aaaagaaaga gaaaatgagt
     4021 gaaaaggaag aactgaagaa agagaatcag ccgaatgctg aaaatccgga gcagaatgat
     4081 gcaacgttga atgaatctga atcaagcaaa gttgaaggcg acgaaaagca atgtcctgat
     4141 gcggttgttg aaactatgga aagtctgcag ctgaaggcca atgagtggca ggacaaatac
     4201 acccggcttt ttgccgattt tgagaattac aaaaaacgca tgcggcagga acgtttcgac
     4261 ttgctgcaga ctgccggttc agagcttatg ttagatattt tgcctgtcat tgatgatttc
     4321 gagcgtggtt tcgccagtct tgctacagcc accgacatcg aagcggttaa gcagggatac
     4381 gagctgattt acaacaaact tgtcaatatt ttgaagcaaa agggacttga accgatggaa
     4441 tcaaagaacg aagtttttga tacagatctg catgaagcca ttaccatgat cgaaagtcct
     4501 gatatgaagg gcaaagtggt tgatgttgtt cagaaaggat acaagatgcg gggcaaggtg
     4561 ctgcgctatg caaaagtggt tgtcggaaat taatacagaa aaagagtgag taaacgtgat
     4621 tattacgagg ttcttggtgt atcgaaaaat gcatcagccg atgagttgaa aaaagcttac
     4681 cggcagatgg ccataaaata ccatcctgac aaaaatccgg atgataaaac tgctgaagaa
     4741 aaatttaaag aagctgcaga agcgtatgaa gtgctaagca atcccgaaaa acgtcagcgt
     4801 tatgatcagt tcggccatgc cggaatgaat ggcgccggcg gctttggtgg cggcatgagt
     4861 atggacgata ttttcagcaa ttttggcgat attttcggtg attttttcgg tggtggtttc
     4921 agtggttttg gtggaggttt tggtcgcagt cgttcttcgc aacgtgtacc gcagggttcc
     4981 aatctcagaa tcagagtaaa actcaccctt gaagaaattg ccaatggtgt tgagaaaaaa
     5041 gtaaaagtaa ccaagagtgt tccttgcgaa agctgcaatg gaactggggc gcgtgctggc
     5101 agtaagccga caacctgtac cacctgtcat gggtcaggcc gcgtgatgcg ggttacaaat
     5161 actttccttg gtcaaatgca aactgcttct acttgtcccc attgcaatgg tgaaggaacc
     5221 atcattaccg atcgttgtcc gtcttgttcc ggaaccggat tgacaaaagg ggaggaagtt
     5281 attgccatta aaattcctgc cggtgtcgaa aacggaatgc agctcagtat gtcgggtaaa
     5341 ggaaatgcag ccccaagagg tggggtgcct ggtgatttga ttatccttat tgaggaagtt
     5401 gaacatgagc tttttaaacg cgatggcatc aatcttttgt acgaacataa tatttcttat
     5461 gccgatgctg tgcttggaac tcagattgat gtcccgacac ttgacggaaa agcaagaatc
     5521 aaaataccag caggcaccat tcccggtaaa atgttcagat tgaagggaaa aggtttgcct
     5581 gctctgaaca gttacgggcg tggtgatttg attgtgagta taaatatttg ggttcctcag
     5641 tctgtgtcga aggacgaaaa gacatttctc gagcagatga ataccaaaaa cggatttcag
     5701 cccgataact cacagaagaa aaaatcgttt tttgacagaa tgaaggaata ttttgattaa
     5761 ccttgcacta tgatttattt cgaagcacag gaagtctcca aaaattttgc aaatcaccgg
     5821 gcattggaca atgtctccat tcaggtgaaa gagcagagca tatttggatt gcttggtccc
     5881 aatggagcag ggaaaaccac tctgatcaga atcatcaatc agattatcgg tcctgatagt
     5941 ggacgtttgt tcattaaagg tcatccgctc gatgtgcagc ataccgaaga cattggttat
     6001 ctgcccgaag aacgcggact gtacaaaaaa atgaaagtgg gcgaacaggc attgtatctg
     6061 gctcagttga aaggaatcag taagtccgat gcttatttgc gtctgaaaca gtggttcgaa
     6121 aaatttggca tcgaagattg gtggaataag cgtgtcgaag agctttcgaa aggtatgcag
     6181 cagaaagttc agtttatcat caccgtaatt catgcgccat cattgctgat tttcgatgag
     6241 cctttcagtg gcttcgatcc catcaatgct gaaatgttga aagaggaaat tcttcagttg
     6301 aaagaaaaag gtgctactat cattttttca acgcacaaca tggcctcggt cgaagaactt
     6361 tgcgatgata ttgcgttgat aaatcagtca aaagtgattt tatctggaaa tgtgcaggca
     6421 atcaagcaaa aatacagctc caatgtgtat cagttgaaat tgcgcgctgc acaaatgtgc
     6481 gatgtatcca atttcagcga gatgggaatt gtcagtggtt ttgaaaatca aagcgattta
     6541 tgttcgttca gaattgagct caatccaaat gtcacttcca acgatttgtt aatggcctct
     6601 atggcttttg gccatgtaac ttcttttgaa gaattgatgc cttcaatgaa cgatattttt
     6661 atttctaagg taaccggtaa caagattcac aaaaaaagcc gtaaatgaaa aaaatcctga
     6721 tcattttacg aagagaatat ctttcgaggg ttcagaaaaa atcattcata gtaatgacta
     6781 ttctcggacc gatacttatg gctgcattat ttgtggttcc tgtttatctg gccaatgtgt
     6841 cggatcagca taaaaaaatt gcggtccttg atgaaacggg tttgtttcat gataaatttc
     6901 cgaattcaaa caaagtgatt ttcgaaaatg tttatatgtc ttatcaggca gccagcgaaa
     6961 atctcgaagc tcttggctat gatgccgttt tgtatattcc cgaatctgtg atcaataacc
     7021 ccaatgccgt gaaaatgagc tctgaaaaag atatgggctt tggcattgtt gatttgattg
     7081 agggcgttat taaaaaagag cttgaagctc atcgtttatc tctttcaggt attgataaaa
     7141 aaattctcga taatgtaaat gtcaatgtaa aaataagcac atatatcttg cgtgagggcg
     7201 aagaggaaca aagtttttct gaaatcagtt atattcttgg catgcttggt ggaattctga
     7261 tttacacttt tatttttctt tatggttctc aggttatgcg cggtgttatc gaagaaaaaa
     7321 caaatcgcat tgtggaggtt attgtaagca gtataaaacc ttttcagctg atgctgggaa
     7381 aaatcgtggg tgtggctttg gtgggtctta cgcaatttgt tctctggatt ggcctgacgg
     7441 ctgtcattgt ttttagtctg cagcaactaa atccggatat gttcaaatat aaggaacctc
     7501 caaaagtgat ggtacaggat aaaggccttt ctcaatcgga tatggcccag caacaggaag
     7561 ctttgcagtt gtcggaaagc agatccaatc agattcttga gggaatatca catattcagt
     7621 ttggaaatat tctgattgtt tttgtttttt attttctttt tggttatttg ctctatgcgt
     7681 ctatgtttgc ggccattggt tctgctgttg acaatgatgc cgatacacag caatttatgc
     7741 tgcctgtaac agttcctctg attttggcgc ttgtcagtct caattttgtt cttaataatc
     7801 ccgatggccc tgtttcgttt tggttgtcaa tgattccttt tacttcgccc attgttatga
     7861 tggcgcgcat tcctttcaat ccgccggtgc ctttgtggga aatcggtttg tcaatggctt
     7921 tccttattgc cggctttatt ttaacaacct ggattgccgg tcgtatctat cgtacaggaa
     7981 tccttatgta tggcaaaaaa ccgactgtca gggaattgtt caagtggatg aaatattagc
     8041 tgcagagagg atggtcacgc atcaacgtga aacgttgcaa gcggagacgc gcgcccagac
     8101 caaggttgag gacaagaact ctcctgccga ggcaggataa attcccatgg aacaatccgt
     8161 gcatccttac ggatcaagag gaagtaagaa tatcgaacgg cacatcattg catttcactt
     8221 cgtaattcaa aaatccttgc ctgcctgact gtctgttgca gttaaaccgg cagtcaggtt
     8281 cagctgcaag ttccgaccga cagcgtagga atgttcatga ttcaaggagg ttaaggtcga
     8341 gaggtttaat cccaaagccc gcaaatttcg tagctttgtt ttgcaatcat ttcaacgcaa
     8401 acgaattttt tattaatgaa tatcaaagaa tatcatcgtc gattatccga tgcattgcag
     8461 gccgcagcca atcccgttgt tgcagcagac gcaaaaaaat acatgaaaaa caagtccgat
     8521 ttcttcggtg ttagttcacc aaaacgcaag gaaatactga cggctttttt aaaggaaaat
     8581 cattttccag ccatccatga tattgaatta tttgcactta tgtgctggga ttcatcccaa
     8641 cgcgaaatgc agtactgctg catggaaata ttatatcgca tgcgtaaaaa acttactccc
     8701 gcacatttgc cgctgttcga aaatatgatc cagactaagg gttggtggga caccgttgat
     8761 tttattgcac catcgctggc gggttttatt ttgaaaaaca atccggaatt gattgacgaa
     8821 accatttcac gctggcagca tcacgacaat atgtggatgc gacgctcagc cattcttcat
     8881 caattaaaat acaaaactga taccgatcaa aaaagattgt tttccatttg caaagcattg
     8941 gcacacgaaa aggaattttt tattcgcaaa gccattggct gggctttgcg tgaatacagc
     9001 aaagtcaatt ctgatgcggt tcgtagcttt gttgcaacaa ccgaattgtc cggtctaagt
     9061 cagcgcgaag cactaaaacg gatttgattt gcctggcacg attttgttga ttataaaaac
     9121 aaattttttt aactttggaa ccatgaaata ttatttgctt ttaattccgt ttattattct
     9181 ctcgtgcaaa agcacaaaaa acatgaaaaa cgaaaaaccg cttattggtt caatcatgta
     9241 tgaataccag gcatcgtcgg tggcgcctca atatcaccga agttatgtgt tattggtatc
     9301 acccgataaa attaaagtca ccgttgacag ttatggaact attctgacgg atacagttat
     9361 tttaatttca ataaaccagt tcaacgaaat tgtggccgag tacgattcgc ttgcgtttcg
     9421 gcctgtacca aaaaaagaca acaggggctg cgtgggtgga acgggtgcca ttttaaaagt
     9481 ctctgacatc agtgatacat tgatttttga tggctatatc agtttctgtg gcgggcagga
     9541 atttggtaat atggaaggtg atgtgaaaaa attgtcagag aaaatcaagt catatattcc
     9601 cgattttgaa ttattgctga agcgtgattg gaagccggag gaaacagatg aatgatattc
     9661 tggaaaattt atcggttttt gatgtgaatg atgaagattt cgagcacaaa tcttatcttc
     9721 acggtcgttt gcatactcaa cgggtgatgg cctgggtatg cgtactggct caaaagctca
     9781 atctgtccga ggatggccga cttgcatttt ttgctgccag gattcatgat ttgggtcgat
     9841 tgactgacgg acgggaacca ggccacggac tgcgttccgc agattattat ctgccgaaat
     9901 acaggattgt ttttgaaaaa tacggtatgc gtagcgagga ttatattacg gtttataatg
     9961 ccgttaaatg gcattcccgc tctgatgaac cggataaaaa tattaaaaat atcgatgtga
    10021 taaagcttct gaaagatgct gatggtcttg atcgtgtacg acttggcgat gatgagcctg
    10081 atattcgttt tttcagaaat gatatttcaa aaagatttgt ctcgcatgcg cggctattgt
    10141 acaacattac cgaagaaaaa gaaaacatgt cgctgaagga aattgtggag ctggcgtttg
    10201 aattgacaaa ttagtttaca gtagtagtct caaaattttt tattgttacg atattccatc
    10261 gttccggcca gttaaaaatc cctcgaaggg ttcgaaaccc ttcgagggat actattttgg
    10321 ccggaatatg gaatcgtcgt gacagcttta gcagttatta caaaatatta tttcggaagc
    10381 tgtttcgatt tcttgttttt attgattcgg gcgatgatga accacaccag tgcaccggcc
    10441 agccagagcg gccacagcgt aatcagcccg ataacgaaca gttgcaatac ttgccagcca
    10501 ctgttgattg cttgcatcag ttttttgccg aatcccggtt tatagttgtc aatgcgttct
    10561 tcgtcaggca gcatcacatg ccgtgttttg tcacggccgt aaatatgcag cgttatggtt
    10621 gcataattga cctgatcatc cagttccagt ttttcaagta aagcttcatc attctgaatt
    10681 tgtttctgaa gctgtgattc catggcagca aggcgatcgc cgctattgcc gcttgtagtt
    10741 aacccgctca cctgaatatt gtagagatcg agtcgttttt ttataagctg cttgcgcaga
    10801 taggccagac tgacatcttc tgctttgatg ttgcgataat cgagaaagtc gatcatggga
    10861 accagacatt tcaaaacagt gtccatgttt tttgcaggca cgcgaacaac catttcattg
    10921 gtgaagtgat aagtggttgt ttcaatcagc gagtcggggc taattcgtgt ggtttctttt
    10981 ccggttactt tacttgataa atctgtgctt gcagtgaatc cgccgaattt ctttacaatg
    11041 tcttctatat tgaatgctgt ttgcgtaaca tccagtgctc tgaatttaag ttctgctgtg
    11101 cgaataagct tgtgggttga gtcaaattta ctcatttggg cagcagacga ggaaatcatt
    11161 gcagcataat cgtctttttt gccttcggca tccgattttt cgattgtttc ggatgttgaa
    11221 gtcggtgcgt aaccgctctt ttggtcttcc tgagctgatg tttttacaat ggcagattct
    11281 tctgaaacaa tttcagattc cccgcttttt gagttttcgt cgtaggtttt cttgccgcca
    11341 cagctgaatg ctacgaatgt cacaataatc aatgtgacaa tcaaattttt cgttttcatg
    11401 ggtttcaggt tttaaggttt tggtttattg gtactaaaag taacaaattc tcagatgaaa
    11461 tattgcgatt atttcataaa tagtgaataa tcttcttata agaccgtata actgcgttaa
    11521 acaatcagaa aaaacatgta ttagatagaa atgaattaaa tataatcgac aaaaggtttg
    11581 aatataagta actgtctaaa atttcaaaat caaaattatt attgccattt ttgttgat
//
LOCUS       DAOPKE010000086        11379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_5974_length_11379_cov_9.446485, whole genome shotgun sequence.
ACCESSION   DAOPKE010000086 DAOPKE010000000
VERSION     DAOPKE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5974_length_11379_cov_9.446485"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKY63_10105"
     CDS             <1..574
                     /locus_tag="PKY63_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01009.1"
                     /translation="YCQRKVLRKFCTVKVKAISFLMRLLPGLFILTSVIAIVGFVSMQ
                     SSEVSASRPMVIPGSADFANASIKNLLPDTPAVFSPANKLVVNGQSVMFAWHNTNAFD
                     NYELMYSWNSNFESAQSVLVKDTVYSLSFTEASTQPLYWKIRTVTADKAYSQWTAVHS
                     ITFQPVVNVWRCDHNCAQCPNPCGRRAPIR"
     gene            complement(576..1754)
                     /locus_tag="PKY63_10110"
     CDS             complement(576..1754)
                     /locus_tag="PKY63_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPF01010.1"
                     /translation="MTPLIIVVLAVLSIVRVIVTKKYKPQRFTLLSGLSAVFYFLFIA
                     GLLNTEHLDKGLFDLQVKLVFLVFPIIFFLSGSILLVENRFRKVLAAFVAGAFASTLL
                     CIGHAAYISLTTNFTFDHFIYAELSFLLHPSYYAMYLNLAIVICSLHLEKKWKVISRP
                     QKTVIASLILYLTIFILFVNSKAGIIITLITFLIIMIRLMVVKRKFVLGSVLLIILFS
                     SVAVIWTTVPYVKSRFQGFLISVSSYDRNLKNSSEGTSERILVWKNASVVALEKLPFG
                     AGTGDVNAALKECYEKNAFAEGSKRAFNAHNQYLQTTIAIGIPGLLSLLLILAILLRR
                     GIQKDQGLIFYFALIVALNFLVESMLETQAGTVFTAFFISLFGMKEISENKNNSETGL
                     "
     gene            complement(1865..3097)
                     /locus_tag="PKY63_10115"
     CDS             complement(1865..3097)
                     /locus_tag="PKY63_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF01011.1"
                     /translation="MKILLINHYAGSPAMGMEYRPYYMAREWIKAGHEVIIVAATFAH
                     VRTIQPQTNKNFQEEIIDGITYLWEKTPTYQGNGVKRILNMFSFYRKLKCHARKLALK
                     YRPDAVISSSTYPMDNYAAAKIVKFSGAKHFYEVHDLWPLSPMELGGYKARHPFIKYL
                     QRAEDFAYKHADAVISMLPKTLEYMQSRGLKPGKWYYIPNGINMEEWSCNTTVSSDVI
                     ARLSTIRDKYSCLIAYTGSIGIANALTTFIDSARLLSNEHIAFIIVGKGPEKKTLLQR
                     AEGIENVFFFDSVPKQQIPDLLSRFDLLYIGLQRQSLFRFGVSPNKLFDYMMAGKPVI
                     QAIDAGNDPVAESGCGISIEPENPQALADAIKTMMKMPFEKRQEMGNAGKTHVLLHHD
                     YSVLADRFLRILSDTINS"
     gene            complement(3168..3695)
                     /locus_tag="PKY63_10120"
     CDS             complement(3168..3695)
                     /locus_tag="PKY63_10120"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01012.1"
                     /translation="MNNNIIIIGAGGHGKVVAEAILSQGKYDLLGFCDDATPPGTIVF
                     GHYKIICNTSQIAHFDFDFFIVAIGNNETRKKIHELLKQQHQGAIVIHPFTSISAQAQ
                     IEPGSVVLPGAVVSYGAHVGENCIIGSNVHIDHETIIGSNSYIRNGSSIGSNVNVASE
                     SYTNTGECIQSFTQF"
     gene            complement(3688..4767)
                     /locus_tag="PKY63_10125"
     CDS             complement(3688..4767)
                     /locus_tag="PKY63_10125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF01013.1"
                     /translation="MYRICYLADGKSIHTRRWCEYFLSQGFEIHLITFRATEIPGVIV
                     HFVDCGNIEVSGNNKKVLLKIPQIRRILRKIKPDIVHAHYATSYGLTGALGGFHPFVV
                     TALGSDVLISPFQNKWYKFLLRFVFRKADWITAMSDPMKEIMISLGAAKNKTETVVFG
                     IDPDIFNDRNRNTPVDTFTIISTRNFEDVYNIDVLIRALNLVNQKIKNIKVHLIGAGS
                     KETQIKSLIGELHLTSIVEMHGRMPQQQIADLLRASHLFVSVSSSDGNNISLNEAMAC
                     GCLSVVSDIPANRQWIADGKNGFFTEAITAEAVAAKILEAYTSYDNLIVVANTLNQKV
                     INEKAIWKTNMQKVEQHYLKLCKHE"
     gene            complement(4789..5697)
                     /locus_tag="PKY63_10130"
     CDS             complement(4789..5697)
                     /locus_tag="PKY63_10130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF01014.1"
                     /translation="MDISIVIVNYNVRDYILKCLDTIFASPLQMSTEVIVVDNNSHDG
                     SVEAIAAAYPQVKLIINKYNDGFSAANNQAFQVAEGRYILMLNPDTELTEDSLHKLFS
                     FLENNAEFSIVAPKLTNTDGSLQQSVWRFPSVCTLFFDMFYLTMFLKKKNYADKDMHL
                     AFEVDSISGAALLFRREVIEKIGMLDETLFWIEDIDFCYRAHKAGLRTLYYPDSQVIH
                     HVSKSAKLNYNISISNQIFSKIKYFRKHKNFVSTFLLTIISLINVLLRFIVFGLLSPF
                     SKIYWRKAKAYLYTLPKVIVPPKGIK"
     gene            complement(5697..6752)
                     /locus_tag="PKY63_10135"
     CDS             complement(5697..6752)
                     /locus_tag="PKY63_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPF01015.1"
                     /translation="MKMLDWLVIVFALSNTIIGTIISQDFMLSAYSIRLTYFPVLFYF
                     VCRFLDAETLLGSYHLIFKWFVVLAVIGIVLYFVFYDTMIAMMQITSPVIPEYFMVRM
                     SSVFWTPVVFSTFMMSAVLYFSYRFVESKKWVWLACIALVSFCLMMSMSRGAIVSTAF
                     GLLVITALYRKYTTIFPWIVIAIAFLFTGYLNASPEDIFMWIFQSTSDTVGLKEGVTR
                     VDLWMNAFGNFNENPFGMGLGNAGHVAARFYDSNASNADVYSTDGWFLKTLLETGIWG
                     LFSYLLFAGAFLIVISRQLKHHKQRPVLVFIVALFLAINLQNIVSNVLDFYLFSYLFW
                     GISGLAANLIYNRSNTI"
     gene            6796..7026
                     /locus_tag="PKY63_10140"
     CDS             6796..7026
                     /locus_tag="PKY63_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01016.1"
                     /translation="MTNANISGSKEKRYSEIFTEVPVPMLSTRNVGKRVANMKNASSS
                     GKNGMFIKYRVFKLRGFSGFCGLYNGFVMQNY"
     gene            complement(7048..8142)
                     /locus_tag="PKY63_10145"
     CDS             complement(7048..8142)
                     /locus_tag="PKY63_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF01017.1"
                     /translation="MTHKPENRKKLLYIMHVSWYWIKQRPHFLALHLSNSFDTTILYP
                     KSVRYIRSKSSAPDNCKGIPQWPLSRFSFIKMLNKALFRHAVKQKLKNTDILWIGNPD
                     DFELISDLVAPDVKVVYDCMDDLCEFPGINASTLKNIISAEQQLINKADVLLASSQHL
                     KNVLQTRYQSAKPIEVVNNAIDISFLNVGKTNPVIEYPIKRDYTDILYIGTISEWFNF
                     PLMLKAMNQFASLRLILIGPKCAPIPEHEHIIHLGSKAHTDLPAYMAKCDALVMPFVI
                     NDLILAVNPVKLYEYLVAGKPVITCHYAEIDPFGSFVFPYDSDEKFLEYIEKLILGKL
                     HAQPEAERIMFLNNNTWAQRAALITSILNG"
     gene            complement(8151..9458)
                     /locus_tag="PKY63_10150"
     CDS             complement(8151..9458)
                     /locus_tag="PKY63_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPF01018.1"
                     /translation="MSKSVFWSLTGSVLSQGLMLLATIVVARILGKTEYGELGMIRST
                     VSMFAVFAGFGLGMTATKYIAEFRYSHPEKTGKIIGLTTLFAGGTGGIISIIILLSAP
                     YIATHSINAPHLVNEIRLGAVMLFFSALNGAQTGTLSGFEAFKTIARVNLVSGLLAFP
                     IQIGCTLLLGLQGSVIGFGLSFCILWILNFFAVRQESKKAGIAIRFSKAWDEWNVLLK
                     FSLPALLSGILVSPVMWACNALLVNKPGGYDEMALFDAANQWRNAILFIPAILAQIAL
                     PMLSAHVHDKPKFNRILKLNIRINVIVATAIAVLVSLLAVLIMKSYGKGFAEGSEVLI
                     VLAGSAVLVAVNNIIGQAIASKGRMWAGFSLNLIWSVILISCTAWFINRGMGAKGIAL
                     GLLISYVLHTITQSVFVHFIMRKQVPTLPPTTNAIKESDHTSA"
     gene            complement(9544..10752)
                     /gene="wecB"
                     /locus_tag="PKY63_10155"
     CDS             complement(9544..10752)
                     /gene="wecB"
                     /locus_tag="PKY63_10155"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPF01019.1"
                     /translation="MMTERPLTKKKILLVFGTRPEAIKMAPLVAEFRKHPEFFDTLVC
                     VTAQHREMLDQVLELFDIVPDHDLNIMHEGQDLTDVTSRVLIGMRDVLEQLKPDLVLV
                     HGDTTTSTTTALAAFYKQIPVGHIEAGLRTYNVYSPWPEEMNRQITGRIAFWHFAPTI
                     LARQNLLSENVPDNQIIVTGNTVIDAMLSGLSIIENRHGVYEEIISDLVAFGLPHERV
                     TQWNGLGSERKMILITGHRRENFGEGIVNICEAIRELSEQFTMVDFVYPMHLNPNVRK
                     PIHEIFGHEIKSPNNNVFFIEPLQYLPFLFLMGKCVLVLTDSGGIQEEAPGLGKPVLV
                     MRDNTERPEALQAGTVKLVGTNKKTIIDEVTTLLTQRDAYNTMAQALNPYGDGKACER
                     IVQKLIQSFE"
     gene            10893..>11379
                     /locus_tag="PKY63_10160"
     CDS             10893..>11379
                     /locus_tag="PKY63_10160"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPF01020.1"
                     /translation="MKIFVVGYMASGKTRFARRLANALGKPFIDLDQVIFDRTQRTPG
                     EWIREYGEEKFRQTELLCLLHCLEQDNFVMATGGGTPCFFDNMNRLNKEGVTIWLKTP
                     IGNIIQRLRNRKGDRPLVATIEGNIDREALEHHYAGRVPFYSKAKIEVVDVDISLVID
                     QI"
BASE COUNT         3372 a         2354 c         2397 g         3256 t
ORIGIN      
        1 ttactgtcaa agaaaggttt taagaaaatt ctgtaccgtg aaagtgaaag cgatttcttt
       61 tttaatgagg ctgttgcccg gactttttat tttgacttca gtcattgcca ttgtagggtt
      121 tgtttcaatg caatcgagcg aagtgagtgc ctccagaccg atggtaattc caggatcggc
      181 tgattttgcg aatgcttcga taaaaaattt actccctgat actccggctg tattttctcc
      241 agccaataag ctggttgtaa acggtcaatc cgtgatgttt gcctggcata ataccaacgc
      301 tttcgacaat tatgagctga tgtacagttg gaacagtaat tttgaatccg ctcaatcggt
      361 tttggtaaag gacacggttt atagtctgag ttttaccgaa gccagcaccc aaccgcttta
      421 ttggaaaata cggacagtta ctgctgataa agcttattcg caatggactg cggttcacag
      481 tatcactttt cagccggttg tcaatgtttg gcgctgcgat cataattgcg cccaatgccc
      541 gaatccatgc gggcgccgtg caccgatccg ttaattcaaa gtccggtttc ggaattgttt
      601 ttattttcag aaatttcctt cattccgaaa agtgaaataa agaaagctgt aaacacagtc
      661 ccggcctggg tttctagcat ggattccacc aggaaattca gcgcgacgat caatgcaaaa
      721 taaaatatta atccctgatc tttttgaatt cctctgcgca ataaaattgc aagaatcagc
      781 aacagactga gaagccccgg aataccgatt gctatggtgg tttgcagata ttgattgtgt
      841 gcattaaacg ctctttttga accctccgca aaagcgtttt tctcgtagca ttcttttaat
      901 gcagcattca cgtcgccggt gccagcgcca aatggcaatt tctccaatgc aactacagaa
      961 gcatttttcc aaacgagtat tctttcgctt gtaccttcac tcgaattttt taaattcctg
     1021 tcgtacgatg aaacagaaat taaaaagccc tgaaaacgtg attttacata aggaaccgtt
     1081 gtccatatca ctgcaacaga agaaaacagt ataatcaata aaacacttcc caaaacaaat
     1141 tttcttttta ccaccatcaa acgaatcatt ataatcagaa atgtaatgag ggtgatgatg
     1201 atgcctgctt ttgaattgac aaaaagaata aaaatagtca ggtaaagtat taatgaggca
     1261 atgacagttt tttgtggtct ggaaattact ttccattttt tctcaagatg cagcgagcaa
     1321 atgactattg ccagattgag atacatggca tagtaggaag gatgcaacag aaacgacagc
     1381 tccgcataaa tgaaatgatc gaatgtgaaa ttggttgtca gcgaaatgta agcagcatgt
     1441 ccaatgcaca gaagtgtgct tgcaaaagct cctgccacaa atgcggcaag tactttccga
     1501 aatctatttt caacaagcaa tattgatcct gaaagaaaaa atatgatagg aaagaccaga
     1561 aacacaagct tcacctgtaa atcaaaaagg cctttgtcaa ggtgttcggt attgagaagt
     1621 cctgcaataa aaagaaaata gaaaacagca cttaacccag aaagcagcgt gaatcgctga
     1681 ggtttgtatt ttttagtgac aataacccga acaattgaaa gcacggccaa taccacaata
     1741 atcagtggag tcactttttt gtaaaacggg atacagaaag acaaaaggat aaatagcaaa
     1801 aagattccat aatccagcga aagattttct tttgagaaga atgagaaaaa tggccggttt
     1861 gctttcatga gtttattgta tcagaaagaa ttctcaggaa acggtcagcc aaaacggagt
     1921 aatcgtgatg taaaagcaca tgggttttcc ccgcgttgcc catttcctgt cttttttcga
     1981 aaggcatttt catcatagtt tttattgcat cagcaagagc ttgaggattt tcaggttcaa
     2041 tactgatacc gcaacccgat tctgcaacag gatcatttcc cgcatcgatg gcctgaatca
     2101 ctggtttgcc ggccatcatg taatcaaaca gtttgttggg ggaaacaccg aaacgaaaca
     2161 gagactgcct ttgcaagcct atatacaaca aatcaaaacg agatagcaga tccggaattt
     2221 gctgtttggg cactgaatca aagaaaaaca cattttcaat tccttctgca cgttgcaaca
     2281 gcgttttctt ttcaggaccc ttgccgacaa taataaatgc aatatgctca ttggaaagta
     2341 atcgcgctga atcaatgaat gttgtcaggg catttgcgat tccgatactc ccggtgtatg
     2401 caataaggca tgaatatttg tctctaatgg tactcaatcg tgcaatgaca tcagatgaaa
     2461 ctgttgtgtt gcacgaccat tcttccatgt taattccgtt cgggatataa taccattttc
     2521 ccggcttcag tccccggctc tgcatatatt caagagtttt gggtagcatg gaaataacgg
     2581 catctgcgtg tttatatgcg aaatcttcgg ctctttgcag gtatttgata aatggatgac
     2641 gggctttata tccgccgagc tccatgggcg aaagcggcca caggtcatgc acttcgtaaa
     2701 aatgttttgc tcccgaaaac tttacaattt ttgccgctgc ataattatcc atcggataag
     2761 ttgatgacga aataactgca tcaggtctgt attttagcgc cagcttccgt gcatgacatt
     2821 ttaatttgcg ataaaagctg aacatgttca gaatgcgctt tacaccatta ccctgatagg
     2881 ttggtgtttt ctcccataaa taagtgattc cgtcaatgat ttcttcctga aaatttttgt
     2941 ttgtctgcgg ctgaatagta cgtacatgcg caaaagttgc tgcaacaatg atgacttcgt
     3001 gtccggcttt tatccattcg cgagccatgt aatatggtct gtattccatg cccatggccg
     3061 gagaacctgc gtagtggtta attagcagga ttttcatgct gacagccccc acgaataatg
     3121 gaaagttttc tcccctttac ataaaatata atttcgcatc atttttttta gaactgtgtg
     3181 aacgattgaa tacactcacc ggtgttggtg taactttcag aggcaacgtt tacattgctt
     3241 ccaatgctgc ttccattgcg aatatagcta ttgctcccaa taattgtttc gtggtcaata
     3301 tgcacattgg agccaataat gcagttttcg ccaacatgtg caccatatga cacaacagct
     3361 ccgggcaata caacacttcc tggctcaatt tgggcctgag ccgaaatact tgtaaacgga
     3421 tgaataacga tcgcgccttg atgttgctgt tttaaaagtt catgaatttt ttttcttgtt
     3481 tcgttatttc caatggcaac aataaaaaaa tcaaaatcaa aatgtgctat ctggctggta
     3541 ttacaaatga ttttataatg gccgaaaacg atggttccgg gaggtgttgc atcgtcgcaa
     3601 aaaccaagca gatcgtattt cccctgactg agtattgctt ctgctaccac tttgccatga
     3661 ccgccggcac ctatgatgat tatgttgtta ttcatgtttg cagagtttta aatagtgttg
     3721 ctctactttt tgcatattgg ttttccagat ggctttttcg ttgatgactt tttgatttag
     3781 cgtatttgcc acaacaatta gattgtcgta tgaagtatat gcttccagaa tttttgctgc
     3841 aacggcttca gcagttatgg cttcagtgaa aaatccattt ttgccgtcag caatccattg
     3901 gcgattggct ggtatatctg acacaacgct taaacaacca caggccatgg cttcgttgag
     3961 tgagatgttg ttgccgtccg atgaagatac actgacaaac aaatgcgacg cgcggagcaa
     4021 gtcggcaatt tgttgctgcg gcatgcgtcc atgcatttca acaatagaag tcaaatgtaa
     4081 ttccccgata agacttttga tttgagtttc tttactgcct gcaccaatca gatgaacttt
     4141 gatatttttt attttttgat ttaccagatt taacgccctg atcaaaacat caatattgta
     4201 cacatcttcg aaattccgtg tactgattat tgtaaaggtg tcaacaggcg tgtttctgtt
     4261 tctgtcatta aaaatatccg gatcgattcc gaaaacaacc gtttcagttt tgtttttggc
     4321 agcgcccagc gaaatcatga tttctttcat agggtctgac attgctgtaa tccaatccgc
     4381 ctttcgaaaa acaaagcgca gtaaaaattt gtaccatttg ttttgaaaag gacttatcaa
     4441 aacatcgctt cccaatgcag taaccacaaa aggatgaaat ccgcccaggg ctccggtgag
     4501 gccataactt gtggcgtaat gcgcatgaac aatatcgggt tttattttgc gcaggatacg
     4561 acggatttgt gggattttca gcaacacttt tttattgttg cctgaaacct cgatattacc
     4621 acaatcgaca aaatgaacga taacaccagg gatttcagtc gcgcgaaaag tgatgagatg
     4681 aatttcgaaa ccctgagaca gaaagtactc gcaccaacgt cgggtgtgaa tgcttttgcc
     4741 gtctgccaga taacaaattc tgtacatata tcaacaaatt cagaggtttt atttaatgcc
     4801 tttgggtgga acgattactt tgggaagagt gtaaaggtac gcttttgctt ttctccaata
     4861 tattttagaa aacggcgaca ataagccaaa aacaatgaac cggagcagaa cattgatcag
     4921 acttatgatc gtgagcaaga atgtactcac aaagtttttg tgttttctga agtattttat
     4981 tttgctgaaa atctgattcg aaatcgaaat attgtaattg agtttggcac tttttgacac
     5041 atggtgaatc acttgtgaat ccggataata aagtgtcctg agtccggctt tgtgagcccg
     5101 atagcaaaaa tcgatatcct ctatccaaaa aagtgtttcg tctaacatgc cgattttctc
     5161 aatcacttca cgacggaaaa gtaaagctgc acctgaaatg ctatcgactt caaatgccag
     5221 atgcatgtct ttatcggcgt agtttttttt ctttaaaaac atggtcagat agaacatgtc
     5281 gaaaaacaag gtgcaaacgc tcgggaagcg ccatacactt tgttgcagcg atccatcagt
     5341 atttgtgagt tttggggcaa cgatactgaa ttccgcattg ttttccaaaa acgaaaatag
     5401 tttatgcagg ctgtcttcag tcaactcagt gtcagggtta agcattagga tatatcgacc
     5461 ttcagccact tggaatgcct gattattggc agccgagaat ccgtcgttgt atttattgat
     5521 aatgagtttt acctgtggat aagcggcggc aatagcttcc acacttccgt catgactgtt
     5581 gttatcgacc acaataactt cagtgctcat ctgcaatggg ctggcaaaga tggtatcaag
     5641 acacttgaga atatagtccc tgacgttgta gttaactatg acgattgaga tgtccatcag
     5701 attgtatttg aacgattgta aataagattg gctgccaggc ccgaaattcc ccagaacaaa
     5761 tatgagaaca gatagaagtc gagtacattg ctcactatat tttgaagatt gattgccaaa
     5821 aacaaagcta caataaatac taaaacaggt ctttgcttgt gatgcttcaa ctggcgcgaa
     5881 ataacaatca gaaaagctcc tgcaaaaagt aaataggaaa acagccccca gattccagtc
     5941 tcgagaagtg ttttcagaaa ccatccgtcg gtcgaataca cgtcagcatt gcttgcattg
     6001 ctgtcataaa agcgtgctgc cacatgacct gcatttccaa gccccattcc aaaagggttt
     6061 tcattaaaat tgccgaaagc gttcatccat aagtctaccc gcgtgacccc ctctttaagc
     6121 cccactgtat ccgacgttga ttgaaaaatc cacatgaaaa tatcttcggg tgatgcattg
     6181 aggtatccag tgaagaggaa ggcaattgca atgacgatcc aggggaaaat ggttgtatat
     6241 ttccgataca atgctgttat gaccagaagc ccaaaagctg tggacactat tgccccgcga
     6301 ctcatcgaca tcatcaggca aaagctgacc aaggctatgc aggccagcca aacccacttt
     6361 ttcgattcta caaagcgata ggaaaaatat aacacagccg acatcataaa agtggaaaac
     6421 accacgggag tccagaaaac agatgacatc cgaaccataa aatactcagg tatcacaggg
     6481 ctggtgattt gcatcatggc tatcatggtg tcgtaaaaaa caaaatacaa gactattccg
     6541 ataactgcca gaacgacaaa ccatttgaaa attaaatgat aagatcccaa caatgtttct
     6601 gcatccagaa agcgacagac gaaataaaac aaaacaggga aataagtaag tctgatacta
     6661 taggcagaca gcatgaagtc ttgcgaaata atggtaccga taatggtatt gcttaacgca
     6721 aacacaatca ccagccaatc caacattttc atttccgtct ttttcttttt ccaaatcaga
     6781 aaaccgagaa ttgcaatgac taatgcaaat atttcaggca gtaaagaaaa aaggtattct
     6841 gaaatattta ctgaagttcc tgttccaatg ctaagcaccc gcaatgtagg aaaaagagtt
     6901 gcaaatatga aaaatgcaag cagtagtggg aaaaacggga tgtttattaa gtatcgggtc
     6961 ttcaaattgc gaggttttag cggtttctgc gggctttata acggatttgt aatgcaaaat
     7021 tactaataaa atgcagtttt tcgatggtca gccgttgaga atgctggtga tcaacgcagc
     7081 tctttgagcc caggtattgt tattcaaaaa cattatgcgc tcggcttcgg gctgggcatg
     7141 cagtttccca aggatcagtt tttcaatata ctccaggaat ttttcgtccg aatcataggg
     7201 aaacacaaat gatccgaatg gatcaatttc ggcataatgg caagtgatga ctggtttacc
     7261 ggctaccagg tattcatata acttaaccgg atttacagca agaatcaaat cattgataac
     7321 aaaaggcatc accagcgcat cgcatttagc catataggcc ggaagatcgg tatgtgcttt
     7381 tgatcctaaa tgaatgatgt gttcgtgttc gggtatggga gcgcattttg ggccaataag
     7441 aatgagtcgc aacgaggcaa attgattcat ggccttcagc atcaatggga aattaaacca
     7501 ttccgaaata gtgcctatgt acagaatatc agtgtagtct cttttaatcg gatattcaat
     7561 caccggattc gtttttccga cgttgagaaa actgatgtcg atggcattgt tcaccacctc
     7621 aataggtttc gcactttgat atcgggtttg cagaacattt tttaaatgct gagaggaagc
     7681 cagcaataca tcggctttgt tgatcagctg ttgttctgcc gaaataatgt tttttaaagt
     7741 tgaagcatta atgcccggga actcgcagag gtcgtccatg caatcataca ctacttttac
     7801 atcaggggca accaaatcgg aaatcagctc aaaatcgtcc ggattgccaa tccagagaat
     7861 atcggtgttt tttaattttt gttttacagc atgtctgaat aaagctttat tcagcatttt
     7921 gataaacgaa aatctgctga gtggccattg gggaatgcct ttacaattat caggggcact
     7981 gctttttgag cggatgtagc gaactgattt ggggtacaga attgttgtat cgaacgaatt
     8041 actcaaatgg agcgcaagaa aatgagggcg ttgtttaatc cagtaccagc tcacgtgcat
     8101 gatgtaaagc agcttctttc tattttcggg cttgtgtgtc atgagtaatt ttatgctgaa
     8161 gtgtggtcag attcttttat tgcatttgtt gtcgggggta atgtgggaac ttgtttgcgc
     8221 atgatgaaat gaacaaatac ggattgcgtg atagtatgca aaacatagga aatcagaagt
     8281 cccagagcga tgcctttagc acccattcct ctgttaataa accaggcagt gcagctaatg
     8341 agtatgacac tccagatcag attgagggaa aaacctgccc acatgcgtcc tttgctggca
     8401 atggcctgcc cgatgatatt attcacggca accaatacgg ccgaacctgc gagaacgatt
     8461 aaaacttccg aaccttctgc aaatcctttt ccgtaagatt tcataatcag aactgcgagc
     8521 aatgatacca acactgcaat agccgtggct acaataacat tgattctgat gtttaactta
     8581 agaatccggt tgaatttcgg tttgtcatga acatgggccg aaagcatggg caaagcaatc
     8641 tgagcaagaa tagcaggaat aaacaggatg gcattgcgcc attgattggc tgcatcaaac
     8701 agagccattt catcgtatcc gccaggtttg ttaaccaaca atgcattaca tgcccacatt
     8761 accggcgaaa ccagaatgcc ggaaagcaag gctggcaaac tgaatttgag taatacattc
     8821 cattcgtccc aggctttcga aaaacgaatg gcaatcccgg ctttttttga ttcctgtcga
     8881 acggcaaaga aattcaatat ccatagaata caaaagctca gaccaaatcc aatgactgag
     8941 ccttgcaggc cgagcaatag tgtacatcca atctgtatcg ggaaagcaag caagcccgaa
     9001 accaaattga ccctggcgat ggttttaaat gcttcgaaac ctgacaatgt cccggtttga
     9061 gcaccattca gggcgctgaa aaacaacatt actgcaccaa gtcggatttc gttgaccagg
     9121 tgtggtgcat tgatgctgtg cgtagcaatg tagggtgccg atagtagaat tattattgaa
     9181 ataattcctc cggtgccgcc cgcaaaaaga gtggtcagcc caatgatttt tcccgtcttt
     9241 tctggatggc tgtagcgaaa ttctgcgatg tattttgtgg cggtcatacc aagcccaaag
     9301 cctgcaaaaa cggcaaacat gctcactgtt gaacgtatca tgcctaattc tccatattca
     9361 gttttgccca gaattcttgc caccacaata gtagccaaaa gcatcagtcc ctgtgaaagt
     9421 actgaacctg tgagtgacca aaatacactt ttgctcatcc gctttccgat atcggatgct
     9481 gcaatccggt catattgcct ttttagaggc aggggaaggc acttgtatag tctggacgtt
     9541 aacttattca aagctttgta ttagtttttg aacaattcgt tcacaggctt ttccatcgcc
     9601 ataagggttc agagcttgtg ccatggtatt gtaagcgtcg cgctgtgtta atagtgtggt
     9661 gacctcatca ataatggttt ttttattggt gccaactaat tttacagtgc cagcttgcaa
     9721 cgcttccggt cgttctgtat tatcacgcat caccaaaacc ggcttgccca aacccggtgc
     9781 ctcttcctga atgccgccac tgtcagtcaa caccagtacg cattttccca tgagaaaaag
     9841 aaatggtaag tattgcaatg gctcaatgaa aaacacattg ttattcgggc ttttgatttc
     9901 gtggccgaaa atttcatgaa ttggtttacg gacattgggg ttgagatgca taggatacac
     9961 aaaatcaacc attgtaaact gttcagacaa ctctcgtatg gcttcgcaaa tatttacgat
    10021 cccctcacca aagttttcgc gtcgatgtcc tgtaatcaga atcattttgc gttctgaacc
    10081 caatccgttc cattgtgtca ctcgctcatg tggcaatcca aatgcaacaa ggtcacttat
    10141 aatttcttca tataccccat gcctgttttc aataatggaa agtcctgaaa gcattgcgtc
    10201 aattactgta tttcccgtta caataatttg attgtcaggc acattttcac tgagtaaatt
    10261 ttgccgggca aggatggttg gtgcaaaatg ccaaaatgca atccgaccag taatctgtcg
    10321 gttcatttct tcaggccagg ggctgtacac attatatgtt cgcaaaccag cttcgatatg
    10381 accaaccgga atttgcttgt aaaacgctgc cagagccgtg gttgttgatg tggttgtatc
    10441 gccgtgtacc agcaccaggt cgggtttcaa ttgttccaaa acatcgcgca ttccaatcaa
    10501 aacccgcgat gtaacatcag tcagatcttg tccctcgtgc ataatattga gatcgtggtc
    10561 tggcacaatg tcgaacagct caagaacctg atcaagcatt tcgcgatgct gggcagtcac
    10621 acaaaccaat gtatcaaaaa attcaggatg ctttctgaac tcggctacca aaggtgccat
    10681 tttgattgct tcgggtcggg taccaaaaac aagcaggatt ttttttttcg ttagaggcct
    10741 ttctgtcatc atgatttttc atcggctttg ctcaggcaaa tttattgata aaaatttaaa
    10801 gctggggttt tatacagggt caacgcattt aaatttccaa aaaaccaagg aaaacctgtt
    10861 gatgattgtt gcggaagttt tatttttgcg caatgaaaat atttgttgtc ggatacatgg
    10921 catcggggaa aacccgtttc gcaaggcggc tggcaaacgc gcttggaaag ccgtttattg
    10981 atcttgatca ggtgattttc gacaggacac agcgcacgcc gggtgagtgg atacgcgaat
    11041 atggcgaaga aaaattccgg cagactgagc tcttgtgctt gctgcattgt ttggagcagg
    11101 acaattttgt aatggcaacc ggtggcggca ccccatgctt tttcgacaac atgaaccgat
    11161 tgaataaaga aggtgtcaca atatggctca aaacgccgat tggaaatatc attcagcgac
    11221 tgagaaaccg aaagggagac aggccactgg tagcaacaat cgaaggcaat attgaccgcg
    11281 aagcgcttga gcaccattac gcaggccggg ttccttttta ttcaaaagcc aagattgaag
    11341 tggtagatgt tgatatttct cttgtgattg atcagatac
//
LOCUS       DAOPKE010000087        11321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6015_length_11321_cov_12.185691, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000087 DAOPKE010000000
VERSION     DAOPKE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6015_length_11321_cov_12.185691"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..810)
                     /locus_tag="PKY63_10165"
     CDS             complement(220..810)
                     /locus_tag="PKY63_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF01021.1"
                     /translation="MIEFLENIDRQLFLAINGLNSNAADFLFYWVSQKFTWIPFYIVL
                     LFLGIRSLKNKWWLLILFVPLLVTVTDQASVHLFKNVFLRYRPCHNTELKELVHLVNG
                     YCGGRYGFVSSHAANSFGIAAFMWLIIRQKYHYWFWILFVAYAGLIGYSRIYLGAHYP
                     SDVAAGAVLGIVSGTFFYYIMQLIFFKKSMKKFEKI"
     gene            complement(803..1801)
                     /gene="obgE"
                     /locus_tag="PKY63_10170"
     CDS             complement(803..1801)
                     /gene="obgE"
                     /locus_tag="PKY63_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPF01022.1"
                     /translation="MAESNFIDYVKIYCRSGKGGPGSAHFYRSKLTPRGGPDGGNGGR
                     GGHVIIRGNSQLWTLLHLKYTKHVHAEDGQNGSENLKTGRDGKDVYIDVPLGTVVRNS
                     ETEEVLFEITRHGEEIVLLKGGRGGKGNDFFKTPTMQAPRFAQPGEPFLESWTVFELK
                     LLADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPNLGVVPYRDDRSFVMADIP
                     GIIEGAHEGRGLGLRFLRHIERNSILLFLIPADSPDALKEYKILLNELKLYNPQLLQK
                     DKIVSVSKCDLIDDAQKKKIATKLKSHHPVFFSSATGMGIPELKDAIWNLMNKLDD"
     gene            complement(1812..2384)
                     /locus_tag="PKY63_10175"
     CDS             complement(1812..2384)
                     /locus_tag="PKY63_10175"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPF01023.1"
                     /translation="MFNMVIFGPPGCGKGTQAARIVQSHGFLHISTGDLIRREINTGT
                     AMGELYNKFISRGMLVPDQIILKELYRFALNHNKEKGIIFDGFPRTLYQAQMLDRIFD
                     KRKMKIGLVISIDVPEDELMNRVLERGKTSGRSDDNETIMNNRLMVYFSQTQPVIDYY
                     RKTKRLVSIDGNQHIDTVAAIIDKTIRNHK"
     gene            complement(2388..3560)
                     /locus_tag="PKY63_10180"
     CDS             complement(2388..3560)
                     /locus_tag="PKY63_10180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPF01024.1"
                     /translation="MINIALFGPPGCGKGTQSKKLLEKYKLTYISTGDMLREEIANDS
                     ALGRQAKEVIEKGGLVDDDIIVQIIENKIKMNPDANGYLFDGFPRTVVQAYILEGLML
                     RMNSSLTSMICLEVPREILVERLSDRASRENRSDDKSMDVINYRLKEYEDKTRPVAEF
                     YMSMHKFHSVDGNGELDIVFERIDAIIQKALDSIWFNFIVFGPPGSGKGTQAKRIAQE
                     LNLVYVSTGSMLRHEIEMNTELGRMAKEYVDSGDIVPDEIAIKLIEEQINKHPDAKGY
                     IFKGFPLTIAQAYILDGVLRKMGGKVTKVFSFQSPTLQNIKRLSKRAETQNARSYDRS
                     LDIIIHRLEVYEQRTAALYDYYSGKNLIKHIDATGDEEEVHQRLKTEIERAFKNLR"
     gene            complement(3564..4100)
                     /locus_tag="PKY63_10185"
     CDS             complement(3564..4100)
                     /locus_tag="PKY63_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPF01025.1"
                     /translation="MNSVTVHNRQFNLYLKHNDIVDAIEKLAARMNEDLKDKNPLFIV
                     VLNGAFVFASELITRFQGDCDVTFVRLASYEGTESSNSVRELMSLNVDVEDRTIVIVE
                     DIIDSGLTMLMAKDKLKAAGARDVLIATLLYKPKAFKHNYYIHYVGIEIPNDFIIGFG
                     LDYDGKGRNLRDIYKIAE"
     gene            4272..4778
                     /gene="purE"
                     /locus_tag="PKY63_10190"
     CDS             4272..4778
                     /gene="purE"
                     /locus_tag="PKY63_10190"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPF01026.1"
                     /translation="MEAKVSIIMGSMSDWPVMEKAAQFFEENMIPFEVQALSAHRTPE
                     QVEIFAKNAAKKGIKVIIAAAGMAAHLPGVIASMTSIPVIGVPIKSSFEGLDALLAIV
                     QMPPGIPVATVGVNGALNAAILAGQILSIADTDLNKRMILYKENLKKKVIQANEELKQ
                     VSFKFKTN"
     gene            4832..5656
                     /locus_tag="PKY63_10195"
     CDS             4832..5656
                     /locus_tag="PKY63_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01027.1"
                     /translation="MKKTLFVILITICFSAAQAQYHTFNASMAGMGGSGIAIDEFQAG
                     MNNQAAWGGLESINIGIGYNSRFMLKELSDRYLSFAMPLGKDKGVLGLNLNQFGYSQF
                     NITKAGIGFARRFGPDFSVGVQFDAIHAGTADEMYGSTTAFTFEGGFQYRLNNKIALG
                     TSVFNPVQVKLNETTGEQLPASISLGMTFSPDKILMLSADVVKEQYEPASLRAGFRYL
                     MGETFCLRAGFSTGPFLVSGGAGLKFNTLMIDISTAYHQVLGISPSITISYAFDKR"
     gene            5659..7746
                     /locus_tag="PKY63_10200"
     CDS             5659..7746
                     /locus_tag="PKY63_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPF01028.1"
                     /translation="MNKPLYWLLLLFFTSLPFFVQAQEPIDSTNENTIEDRIENIAEN
                     TDAEIDYSSLTETLKYFRKHPLNLNRADREELEELGLLNAIQIDNLLRHIEKNGALIS
                     LYELQSIDGFDLATIYSILPYVKIFGDNERKTWNLNEILKQSKSTLFVRYTNILQEQA
                     GYAPISDSALAESPNSRYLGSDYKLYTRYKFAYYNMLSFGITAEKDYGEEFFSGNQKQ
                     GFDYYSAHFFIRDIGPLKTLAIGDYSLQFGQGLALWSGLSYGKSAEAINIKKSGRGIS
                     PYSSVDENLFMRGFATQFNLKPFSIYAWVSRKMLDANVESGDSMNTEEFVITSLQESG
                     LHNTQSTIVDKDQISELIAGGRIETSMQRVKLGAMGYYTHYGQSIAPGDQLYEYYNFS
                     GNENLNFGLDYSWIIKNANFFGEAGISKNGGKAFLNGVMLSPDRLFTISILHRYLEPD
                     YHVFYASALQEASTLKNEHGLYLGDELKFSRKWRFNSYVDLFTFPWLRYGVDAPSSGI
                     EMLAQINFRPTKRAEFYGRFKQENKEMSVTQEHISILEPTIKRSYRINASFPISESIT
                     LKSRAEYLTFQEGDGDQRDGFLIYQDINFRKPGSRINFSARYALFDTDTYDERIYAYE
                     NDVLYGYSIPGFYSKGSRVYLLIRWTATKHLDFWARIAHTNYVNKDVVGSGLEEIQGP
                     SKTELKIQMRVTF"
     gene            7805..9790
                     /locus_tag="PKY63_10205"
     CDS             7805..9790
                     /locus_tag="PKY63_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPF01029.1"
                     /translation="MFKNHPKGLLILALANMGERFGYYTMLAILTLYIQAKFGLSSTN
                     TSLIYGTFLSLVYFLPLLGGILADKVLGYGKTILLGIVVMFSGYLLLALPSSANTAGY
                     IMMFGALFLIALGTGFFKGNLQALVGNLYESDLYKGKRDIAFSIFYMFINIGAFFAPS
                     AANSINNAILAQSNYTYDANIPKNYVSLNDEVVDKTAFCADLFSNEKDAAKMTGKDSA
                     AVISKAKKKDGVIFQEDKDKILLKLKTAGLVQLSAANQLPDSIKISSEEYRVFNSNME
                     SDKAAKEKVIAKVNSITVTDPKLGGDPKKPDAFASNFAKKYVNESLSKSYNLAFGVAC
                     ISLVLSLLIFLGFRKTWKSVDKTHKQQLKEAAKTGDSSNVVILTKAQVRERMIALFLV
                     FFVVIFFWMSFHQNGLCMTFFARDYTVSTISPTTNMLFTLLALLPFILFTYGIYLATM
                     GLFGGKRSPGMGILLSFIGLGGLAAYYFIVVKDAVGIVPITPQIFQQFNPLFIVLLTP
                     LFVALFTYLGRKGKEPSAPRKIGYGMLIAGLGFIILLVASLGLPAPSDIQGVSNVLVS
                     PVWLIGTYFTLTIAELFLSPMGLSFVAKVAPPQYKGMMQGGWLAATAIGNYLVGILGT
                     FWDKLPMYAFWGVLVVCCLLSAAFIFSIMKRLEKATN"
     gene            9818..10783
                     /locus_tag="PKY63_10210"
     CDS             9818..10783
                     /locus_tag="PKY63_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262172.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPF01030.1"
                     /translation="MAAFFLAMNNYTPIIKPFTLLSDYLRQTYGGRLQKVSVDAGFTC
                     PNRDGSAGIGGCTYCDNSAFNPSYCSPLKSIRQQVLEGIEFHAKRYRKAQQFLVYFQP
                     FSNTYATTEKLHSLYSEALAIENVAGISVSTRPDCFNRETAELLAGIADNKVVLLELG
                     IESVRDKTLQRINRGHDFECTKKAFALAAEYGLFVTGHYIIGLPGESRNEILSDVSIL
                     NNLPMNALKLHQLQIVRNTAMEKDYLIHPKDYQLFELAEYVDFIVSFAELLRPGLLID
                     RFAGEVPPTFLRAPDWGLIRYDQVLKMIEKKFVVRNTHQGANWKM"
BASE COUNT         3437 a         2416 c         2265 g         3203 t
ORIGIN      
        1 gactacagat atgtgactcc tccggagtcc tccaaaaatg attgcactga tgtatcaatc
       61 atgttgtgat attacctgcc cgacaacaga cgggttcata gatatccggt aaagccgtac
      121 aagccggatt gcatacacct gtcagattta atgcaataaa atatagctat cggaatttga
      181 acttttttta ggcattattg caaagattca aaataatcat cagatttttt cgaacttttt
      241 cattgatttt ttaaaaaaaa tcagttgcat gatgtaataa aaaaaagttc cggaaacaat
      301 ccccaataca gcaccagcag ccacatcgga aggataatgg gcgccaagat aaatacggct
      361 gtagccaatc aatccggcat aagcaacaaa caaaatccag aaccaataat ggtatttttg
      421 tctgataata agccacataa aagcagcgat tccgaaacta tttgccgcat gcgatgaaac
      481 aaaaccatac ctgcctccac aataaccatt aacaaggtga accagctctt tcagttctgt
      541 attatggcaa ggtcggtaac gaagaaatac atttttgaac aaatgaacgg atgcctgatc
      601 cgtaaccgta acaagcaaag gaacaaacaa gatgagcagc caccatttat tcttcagcga
      661 acggataccg agaaacaaca acacaatata aaaagggatc catgtaaatt tttggctgac
      721 ccaataaaat aaaaaatcag ctgcatttga attcaggcca ttaatagcaa gaaacaactg
      781 ccgatcaata ttttcaagaa actcaatcat ccaatttatt cattagattc caaatggcat
      841 ctttaagttc ggggataccc attccggtag ccgatgaaaa gaaaaccggg tgatggcttt
      901 taagttttgt ggcaattttc ttcttttgag catcatcaat caaatcgcac ttcgatactg
      961 atacaatttt gtctttctgc aataattgcg ggttgtatag ctttaattca ttgagcagaa
     1021 ttttatattc tttcagggca tccggtgaat cagccggaat gagaaacaac aaaatactgt
     1081 ttcgttcaat atgacgcaga aagcgcaagc caagccccct tccctcgtgt gccccttcaa
     1141 taattccggg aatatccgcc atcacgaatg agcggtcatc gcggtaaggg acaacaccaa
     1201 gatttgggac cagagtagta aaagggtaat ctgctatttc gggttttgca gccgatacta
     1261 cagacagcag cgttgattta ccggcatttg gaaagccaac caatccaaca tcggcaagca
     1321 gcttgagttc aaaaacagtc caactctcaa gaaagggttc acctggctga gcaaaacgcg
     1381 gagcctgcat agtaggagtt ttgaaaaaat cgttgccttt tccgccgcgg ccacctttga
     1441 gcaggacaat ctcttcgcca tgccttgtaa tttcgaaaag cacttcttct gtttcagaat
     1501 tacgaacaac agtacctaag ggaacatcga tatacacatc tttgccatcg cggccggtct
     1561 ttagattttc gctgccattc tgaccatctt cggcatgaac gtgcttggta tatttaagat
     1621 ggagtaaagt ccagagttgt gagttgccgc ggattatcac atgaccgcca cgcccaccgt
     1681 ttccgccgtc gggaccaccg cgcggagtca gttttgaacg ataaaagtgt gctgaaccag
     1741 gtccaccctt accgctacgg cagtagattt tcacataatc gataaaattg ctttctgcca
     1801 tagttttaaa tttatttgtg atttcggatg gttttatcga taatggccgc tacagtatcg
     1861 atatgctgat tgccatcaat cgaaaccaac cgttttgttt tccggtagta atcaatcacc
     1921 ggctgcgtct ggctaaaata taccatcagc ctgttattca tgattgtttc gttatcatcg
     1981 ctacgacctg aagttttacc acgttcaagc acccggttca tcaattcatc ttcaggcaca
     2041 tcgatggaaa taacaagtcc gattttcatt ttccgtttat caaaaatgcg gtcaagcatt
     2101 tgagcctgat agagtgtacg aggaaatcca tcgaaaataa tacctttttc cttattgtga
     2161 ttcagcgcaa aacgatatag ctccttaaga ataatttgat cggggacaag cattccacga
     2221 cttataaact tattgtaaag ctcacccatg gctgttcccg tattaatttc gcgacggatg
     2281 agatcgcctg tggaaatatg caaaaaccca tgactctgca caatccgtgc agcctgagtt
     2341 cctttaccac agcccggcgg gccaaagata accatgttga acatatgcta cctcaaattt
     2401 ttaaatgcac gttcaatttc agttttcaag cgctgatgca cttcttcctc atccccggta
     2461 gcatcaatat gtttaatcag atttttacct gaataataat catacaaagc tgcagtacgt
     2521 tgttcgtaaa cttcgagccg gtgaataatt atatcgagac tacgatcgta tgagcgagca
     2581 ttctgcgttt cagcgcgctt ggacagtcgt ttgatatttt ggagcgttgg agactgaaag
     2641 gagaaaacct ttgttacttt tccgcccatt ttgcgcaaca ccccgtcgag aatatatgcc
     2701 tgagcaatgg ttagcggaaa tcctttaaaa atatatcctt tggcatcggg gtgtttgttt
     2761 atttgctctt cgattaattt aatggctatt tcatcaggaa caatgtcacc actatctaca
     2821 tattctttag ccattcgacc caattccgta ttcatttcaa tttcgtggcg aagcattgat
     2881 ccggttgaga catagaccag attcagttcc tgcgcaatgc gtttggcctg agtccccttg
     2941 cccgaacccg gagggccaaa aacaataaaa ttaaaccata tactatcgag tgccttttga
     3001 ataatggcat caatccgttc aaaaacaata tccagttcac cattgccatc cacagaatgg
     3061 aacttgtgca tactcatata gaattcagca accggtctgg ttttatcttc atattccttg
     3121 agccggtaat tgataacatc catgctttta tcgtcggagc gattttcgcg gctggcacga
     3181 tctgaaagac gctctacaag gatttcgcgg ggaacttcga gacaaatcat tgatgtcagc
     3241 gaagaattca tccgaagcat caatccttcg agaatataag cctgaacaac agtacgtgga
     3301 aaaccatcaa ataaataccc attggcatca ggattcattt tgattttatt ttcaataatc
     3361 tgaacaatga tgtcatcgtc aaccagcccc cctttttcga tgacttcttt tgcctgacgg
     3421 cccagagcag agtcattggc aatttcttca cgaagcatat caccggtaga aatataggtg
     3481 agcttatact tttcaagcaa ctttttggat tgggttcctt ttccacaacc cggagggcca
     3541 aacaaagcaa tattgatcat gggttattca gcaattttgt aaatatcgcg caaattgcgg
     3601 cctttgccat cataatcgag tccaaaaccg ataataaaat cgtttggaat ttcgataccg
     3661 acataatgaa tgtaatagtt atgcttaaac gcttttggct tataaagcaa cgttgcaata
     3721 agaacatcac gagctcctgc ggccttgagt ttatctttgg ccatcagcat agtcaatccc
     3781 gagtcaataa tatcctcaac aatcacgatg gtccggtctt caacatcgac attgagactc
     3841 atgagttcgc gcacggagtt tgagctttcg gttccttcgt aactggccaa ccgaacaaaa
     3901 gtaacatcac aatcgccctg aaaacgagta atcaactccg acgcaaaaac aaatgcacca
     3961 ttgagtacaa caataaataa tggattttta tctttaagat cctcattcat tcttgctgcc
     4021 agcttttcga tggcatcaac aatatcgttg tgtttcagat acagattgaa ctgccggttg
     4081 tgtacagtta cagagttcat attttcaaat taagatgcaa atttaatgaa taaaaccgga
     4141 ttatagttgg tttttcctat cgaacgcaaa tcaatataca gcgataaaaa agcacgccct
     4201 gtaacagaat gaaaaataga gaatcatcat tacgaaatta ttattatgtt tgtaggttaa
     4261 aacaaaactg tatggaagca aaagtgagca taatcatggg cagtatgtct gattggcctg
     4321 ttatggaaaa agccgcacaa tttttcgaag aaaacatgat cccgtttgaa gtacaggcat
     4381 tatcggcaca ccgcacaccg gagcaggttg aaatatttgc aaaaaacgca gctaaaaaag
     4441 gcataaaagt gattatcgcc gcagccggca tggcagctca tttacccgga gtaattgcat
     4501 ccatgacatc cattcctgtg attggtgttc ccataaaaag ttctttcgaa ggacttgatg
     4561 ctctgctggc cattgttcaa atgcctcccg gcattcctgt ggccacggtt ggagtcaacg
     4621 gtgcactcaa tgccgctatt ctggcaggac aaatactttc cattgccgat actgacctga
     4681 ataaacgaat gattttatac aaggaaaacc tgaagaaaaa agtcattcag gccaatgaag
     4741 agctcaaaca agtcagtttt aaattcaaaa caaattgatt attcagaatt aaatatgaat
     4801 aacaagtcaa atcaaatata aaaaaagttg tatgaaaaaa acgctctttg tcattttgat
     4861 aacaatatgt ttttcggcag cacaggcgca atatcacaca ttcaatgcat ccatggcagg
     4921 gatgggcggc agtggaatcg ccatcgatga attccaggcc ggaatgaaca atcaggcggc
     4981 ctggggcgga cttgaatcaa taaatattgg tatcggatac aattccagat ttatgctcaa
     5041 agaattatca gatcgttact tgtcttttgc catgccactg ggaaaagaca aaggcgtatt
     5101 gggattgaat ctgaatcagt tcggatattc tcaatttaat attacaaaag caggaattgg
     5161 ttttgcgcgc agattcgggc ccgatttttc ggtcggcgtt caatttgacg ccatccatgc
     5221 cggaacagcc gatgaaatgt atggaagtac gaccgcattt acttttgaag gcggatttca
     5281 ataccggttg aataataaaa ttgcgttggg aacatctgta ttcaatcccg tacaagtgaa
     5341 actgaatgaa accacaggag aacaactgcc ggcatctatt agtctgggaa tgacattttc
     5401 gccagataaa atactgatgc tatcggccga tgtggttaaa gaacaatacg aacccgcatc
     5461 gctgcgggct ggattccgtt atctgatggg cgagacgttt tgtctccgcg ccgggttttc
     5521 gaccggtccg tttttggtat caggcggcgc cggcttaaaa ttcaacacac tgatgataga
     5581 tatctctacc gcttatcatc aggttttagg catctcgcca tccattacaa tttcatatgc
     5641 atttgataaa agataatgat gaataaacca ctatattggc tcctgctgtt attttttact
     5701 tccctgccat tctttgttca ggcacaggaa cctattgaca gcacaaatga aaacaccatt
     5761 gaagaccgca ttgaaaatat tgcggaaaac actgatgcgg aaatcgacta ttcaagtctg
     5821 acagagactc tcaaatactt ccgtaagcac ccgctcaatc taaaccgggc agacagagaa
     5881 gaactcgaag aactcggcct tttaaatgca atacagattg acaatctgct ccgccacatt
     5941 gagaaaaacg gcgcactgat ttcgctatat gaattacaaa gcatcgatgg atttgatctt
     6001 gcaacaatat attccatttt gccttatgtg aaaatttttg gcgacaatga acgaaaaaca
     6061 tggaatctga atgaaatcct gaaacaatca aaaagcacat tatttgtgcg atatacaaat
     6121 atacttcagg aacaggccgg ttatgcgcct atcagcgaca gtgcgctagc cgaaagtcca
     6181 aattcaagat atctcggcag cgattacaaa ctttatacgc gttataaatt tgcgtattac
     6241 aacatgctta gttttggcat cacagccgag aaagactatg gtgaggaatt tttcagcgga
     6301 aatcaaaagc aaggatttga ttattattcg gctcactttt tcatcagaga cattgggcca
     6361 ctgaaaacat tagccatagg cgattattcg ctgcaattcg gacaaggact tgctctgtgg
     6421 tcaggactat cttacgggaa atcggccgaa gcaataaaca taaaaaaaag tgggcgcggt
     6481 atctcacctt acagttcggt tgatgaaaac ctgtttatgc gcggttttgc gacccaattc
     6541 aatttaaaac cattttccat ttatgcctgg gtcagccgca aaatgctgga tgccaacgtt
     6601 gaaagtggag attcgatgaa taccgaagag tttgtaataa caagtcttca ggaatcagga
     6661 ctgcacaata cccaatcgac cattgttgac aaagatcaga tcagtgagct cattgccggc
     6721 ggccgcatag aaacatcaat gcaaagagta aaacttggag ctatgggcta ctacacacat
     6781 tatggacaat ccattgcgcc gggagatcaa ttatacgaat actacaattt ttcaggaaac
     6841 gagaatctga attttggtct tgattatagc tggattatta aaaacgcaaa ctttttcggc
     6901 gaagcaggta taagtaaaaa cggaggcaaa gcttttttaa acggagtgat gctttcaccc
     6961 gacaggttgt ttaccatatc tatacttcac cgctatctgg aacccgacta ccacgtattt
     7021 tacgcttccg ccctgcaaga agcatccacc ctcaaaaatg agcacggtct ctatcttggc
     7081 gacgagctga aattctcacg caaatggcgc ttcaacagct atgttgattt gtttacattt
     7141 ccctggctgc gctatggcgt tgatgcgcct tccagcggaa tagaaatgct ggctcagatc
     7201 aatttcaggc caaccaaaag agccgaattt tacggtcgtt tcaagcagga aaacaaagaa
     7261 atgagtgtaa cacaggagca tatttcgatt ctggagccaa ccattaagcg ctcataccgc
     7321 atcaatgctt catttccaat atctgaaagc atcacactta aatcacgtgc cgaatacctg
     7381 acatttcagg aaggcgatgg cgatcagcgg gatggctttc tgatttatca agacatcaat
     7441 ttccgcaaac caggcagccg gatcaatttc agtgcacgat acgccctgtt cgacacagac
     7501 acatacgatg aacgtatata tgcctatgaa aatgatgtat tgtatggata ttccattccc
     7561 ggattctaca gcaaaggcag ccgtgtatat ctgctgatca gatggacagc caccaaacat
     7621 cttgatttct gggcacgcat agcgcacacc aactatgtaa acaaggatgt agttggcagc
     7681 ggacttgaag agattcaggg accttcgaag actgaattaa agattcagat gcgggttaca
     7741 ttttaatgct ctttcaaaaa ttgttttatt tttgtcgaag aaaattcact aaaactctca
     7801 aattatgttc aaaaatcatc cgaaaggatt attaatcctt gccttggcaa atatgggaga
     7861 gcgatttggc tactatacca tgttagccat tttgactctt tatattcaag cgaagtttgg
     7921 tttatcatca acaaacacca gcttaattta cggaacattt ctatcgcttg tttatttcct
     7981 tcctttgctt ggaggtattc ttgctgacaa agtgttggga tacggaaaaa cgattcttct
     8041 cggaattgta gtaatgttta gtggttatct gctacttgca ttaccttctt cggccaatac
     8101 tgccggttat attatgatgt ttggagcatt gtttttaata gctcttggaa ctggcttttt
     8161 taaaggtaat ttgcaggcgt tggttggtaa tctttatgaa tcagaccttt acaaaggtaa
     8221 aagagatatt gccttctcga ttttctacat gtttattaat attggggcct tcttcgctcc
     8281 gagtgcagca aattcaataa acaatgcaat tcttgcgcaa agtaattata cctacgatgc
     8341 aaacattccg aaaaactatg tttcactgaa tgacgaagtt gttgataaaa cagctttttg
     8401 cgctgatttg ttttcaaatg agaaagatgc agcaaaaatg accggaaaag acagtgccgc
     8461 tgttatttca aaagccaaaa agaaagatgg tgtaattttc caggaagaca aagataaaat
     8521 attgcttaag ctcaaaacag ctggtttggt tcagctttca gcagcaaatc aactacccga
     8581 ttccataaaa atttcttctg aagaatacag agtgtttaac agtaacatgg aatctgataa
     8641 agcagccaaa gaaaaagtaa ttgcaaaagt gaacagtatt actgttacgg atccaaaatt
     8701 aggtggagat ccaaaaaaac cggatgcttt tgcttcaaat tttgccaaaa agtatgtgaa
     8761 cgaatctctt tcaaaatcat acaatcttgc atttggagtt gcctgcataa gcctcgtgct
     8821 ttctctattg attttcttag gcttccgcaa aacatggaag tccgttgaca agacacataa
     8881 acaacaactt aaggaagctg ccaaaaccgg tgatagttcc aatgttgtaa ttttgaccaa
     8941 agctcaggtt cgtgaaagaa tgattgcttt gttccttgta ttttttgtcg tcattttctt
     9001 ctggatgtcc ttccatcaaa acggattatg tatgacattt tttgccagag actacacagt
     9061 cagcacaatt tctccgacaa cgaatatgtt gttcacattg ctggcattac ttccattcat
     9121 actgtttacc tatggaattt atcttgccac catgggattg tttggcggaa aacgctcgcc
     9181 tggcatggga attcttcttt ctttcatagg acttggtggt cttgcagcct actactttat
     9241 tgtagtaaaa gatgctgttg gaattgtacc cattacgcca cagatattcc agcaatttaa
     9301 tccactattc attgtcttgc tcaccccgct ttttgtggca ttattcacct acctgggaag
     9361 aaaaggcaaa gaaccttcag caccgcgaaa aatcggttat ggtatgctga tcgccggcct
     9421 tggatttata attctgctag tcgcatctct tgggcttcca gctccatccg atattcaagg
     9481 cgtatccaat gttttggtat ctccggtatg gctgattggc acttatttca cattaaccat
     9541 tgcggaactc ttccttagcc cgatgggatt gtcatttgta gctaaagttg ctcctcccca
     9601 gtacaaaggt atgatgcaag gtggctggtt agctgcaaca gctattggaa attatcttgt
     9661 tggtattctg ggaactttct gggacaaatt accaatgtat gctttctggg gcgttctggt
     9721 tgtttgctgc ctgttatcag ccgctttcat tttctcaatt atgaagcgtc tggaaaaggc
     9781 aaccaattaa aattcttaca ttctaaaagg ctgctttttg gcagcctttt ttttagcaat
     9841 gaacaattat acacccataa tcaaaccctt caccctcctc tccgactacc tccgccagac
     9901 ctatggcggg cgactgcaaa aagtcagtgt cgatgcggga tttacatgcc ccaaccgcga
     9961 tggaagcgcc ggaatcgggg gctgtaccta ttgcgacaac agcgccttta atccttcgta
    10021 ttgctcgccg ctaaaaagca tccggcaaca ggtattggaa ggaattgaat tccatgcaaa
    10081 aaggtaccgc aaggcgcagc aattccttgt ttattttcag ccgttctcga atacatatgc
    10141 cactactgaa aaactccact ccctatattc agaagctctt gccattgaaa acgttgcagg
    10201 aatcagtgtc agtacccgcc ctgattgctt taatcgtgaa acggcagaat tacttgccgg
    10261 cattgccgat aataaagttg ttctgctgga actgggaatc gaaagtgtcc gcgacaaaac
    10321 actgcagcga atcaaccggg gacatgattt tgaatgcaca aaaaaagcat ttgcactcgc
    10381 cgcagaatat ggactttttg taaccggaca ttatattata ggacttcccg gcgaaagccg
    10441 caatgaaata ctgtccgatg tgtcgatatt gaataatctt ccgatgaacg cgctgaagct
    10501 tcatcagttg cagatagtgc gaaatacagc catggagaaa gattatttga ttcacccgaa
    10561 ggactaccag ctgtttgagc tcgccgaata tgtcgatttt attgtttcat ttgcagaact
    10621 gctgcgaccg ggcttgctga ttgaccgctt tgcaggcgaa gtcccaccaa catttctgcg
    10681 tgccccagac tggggattga ttcgttacga ccaggttttg aaaatgattg aaaaaaaatt
    10741 tgtggtgcgc aatacgcatc aaggcgcaaa ctggaagatg taagaattat gagtgggtga
    10801 ccttcgacgg agttaaccca ctgccgacat ttcgacggag tttatcctgc aggacgcagg
    10861 gctcaatgtg acagcggcgg ggctcaggat aaaatttttg ttttgtgatc agatttctaa
    10921 ataaaaaaag ccccttgcgg ggctcacgtt atctgttatg atttggaggg gcttattggg
    10981 ggtgaaaaag caaatttatt ttcgcgtctt ccacgaaaca aagatacatt ggttagcaaa
    11041 cagatttttt ttacttattt atcatgcagc attttgtgac cttttgcaac aaccaataaa
    11101 aaataaattg aaaagtcacg cgaacagatc ctatatatat ttgtatatca gattcttatc
    11161 attgctgatt atttgtttgt taaaacaaag gtcgaatgaa gcatccatgg gtgcaactta
    11221 tcaaatacaa ctcaaaaaaa acaaataaaa tactgtataa caagacttta atcaggaaat
    11281 acaacacaat aatcaaaaga agataactta tacctttcta a
//
LOCUS       DAOPKE010000088        11219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6077_length_11219_cov_12.360265, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000088 DAOPKE010000000
VERSION     DAOPKE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_11219_cov_12.360265"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(699..2330)
                     /gene="pckA"
                     /locus_tag="PKY63_10215"
                     /pseudo
     CDS             complement(699..2330)
                     /gene="pckA"
                     /locus_tag="PKY63_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
     gene            complement(2938..4803)
                     /locus_tag="PKY63_10220"
     CDS             complement(2938..4803)
                     /locus_tag="PKY63_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPF01031.1"
                     /translation="MMESLLISNPSKGSERFEGCELLSPARDAEHGMAAINFGADAVY
                     IGAPKFGARAAAGNSTADIETLCNYAHRFHAKVYVALNTILYDDELAEAEKLIHDIYN
                     AGADALIVQDMGILEMDLPPIALHASTQTHNYDVRRIKFLQDVGFSRVILARELSLKQ
                     IAEVRSQTNIELEAFVHGALCVSFSGQCYMSAFHGHRSGNRGECAQPCRLKYDILDDK
                     RNIIESNTHALSLRDMNRSAHLGEMMAAGVTTFKIEGRLKDMNYVKNITAHYRRKLDE
                     IIEHNEKYKISSLGRCQPNFEPDPEKSFNRGFTEYFLHGRTEKVSAHTSKSLGKRLGQ
                     VSESGWNFIKIDNCPEISNGDGLCWFDENGELKGFAVQYADNGKIFSDDPINIAKGTE
                     IYRNLDLAFNRQLAAAGSARRIPVNMIFGETDEGFELTISTDDGRYSLNKKIAIEKVP
                     ARDGQKASDTIKTQLSKTGSTFYDVASFDLKWGSPLFFPIGELNRIRREAFEEFDQML
                     LSAYVREEKQIVPNDTPYFENETDYRSNISNALSARFYKRHGVVKTEPAFELSDDHSG
                     QTLMTTKLCLRYETGCCPTYQKAQPELDPQHLAQGEKLFRLEYDCMSCVMKINEA"
     gene            complement(4800..8183)
                     /locus_tag="PKY63_10225"
     CDS             complement(4800..8183)
                     /locus_tag="PKY63_10225"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPF01032.1"
                     /translation="MQSNANELNPLENNSFKLTDLVDIGMLKELSEKFTEITGLTIGL
                     FEYPDQTILFKTGWKKVCTDFHMAYPEAAKHCLICNRKLFDRLERQGQLVIEKCGNGM
                     YDCATPIFIQDKLIACITTGQLLLEKPDNSFFRAQAKKYGFNEEKYIRAFEEVKVISE
                     KDLLLATSFLGQIATYISESGYNNLVIRTEKARLEQEFDKRQKITEALSKSEHKIRTV
                     LALAPEAFFHGDSNGQFMDVNEATIELTGYSREELLKLNMKDLFTSNVLDAEPLRYDI
                     LLTGKTLTKRRLLKHKKGNLVHIEMSSRLMPDGTYQCFAKDITESILAQEALNKSESR
                     LRMFFECSQEGLIFHDKGIVVDVNIAMLEMSGYTDEKELVGKNIIDYISPAYKEKVLN
                     NVLGEKTDKYEAEIIRRDGTIIPVEIMPRHAEFSDGRKLRITSLTDITERKKAEQMQN
                     INARRTMALLQLSEMINAPLNEITNFVLEEVISLTGSKIGYLAFLNETEDVLTMHAWS
                     KSAMEECNVSDAPREYLVDKTGLWGESVRQRKPVITNDYSADNPWKKGYPEGHVTLKR
                     HLSVPVFDNDRIVAVLGVGNKEDEYYDECIKQLTILGSAMWSHIARQRVMKELLESRE
                     QFRLITENSNDMIVAFDAESGIISYLSPGSGRIKKFDAEDVIQHTFFDFIHPDDLPSI
                     LESQKHILSGCDLPLLKHRVMMKDGSFVWYESSVKLVKNAISGKNEIVAVCRDISEKV
                     KTEQLIQEKEFAEAANKAKSEFLANVSHEIRNPLNSIVGMAKTLSRTNFNKDVQEIVE
                     AILTSSDNLLHIINDVLDLSKIEANRIELEDKDFNVNAVFGEVSSVYKSVAEGKGIKF
                     EIRKDASVPARLRGDGNRFRQMVINLTGNAVKFTDKGSVILHVSASTAEDGQTIITTE
                     VSDTGIGIKKEDFGKLFHSFTQLDSSTTKKFSGTGLGLAIVKSFAEMMGGDIKVESEF
                     GHGSRFILTIPFNAPLSDAEETNIVTDEPVQSECKVLLVEDDALNQMYLKGFLSSKGM
                     IVDSAYNGLQALEKYDAEKYDIILMDGQMPQMDGFEATRIIREKETQSGIHTPIIAIT
                     GYAISGDEERFLKVGMDSYITKPFDENRLIEVIKKLTGK"
     gene            8325..9164
                     /locus_tag="PKY63_10230"
     CDS             8325..9164
                     /locus_tag="PKY63_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPF01033.1"
                     /translation="MKWQGRQGSSNVEDKRGKSKGLLGGGIGTIIIIIVIYLLGGDPS
                     GIMQNATDPSNQTTEEYNPSAKEQELAEFSSVVLKETEDTWTKLFNEQGKSYRCPKLV
                     LFSEAVQSGCGAASANSGPFYCPADEKVYINLQFFEELGSRYGAPGDFAMAYVIAHEV
                     GHHVQLLLGINEKYESYRNRLSETEFNKMTVRLELQADYFAGVWAHYAERMNLLEEGD
                     LEEALTAASAVGDDRLQKQAQGHVNPDTFTHGTSEQRSRWFMKGFRSGDMYGGDTFAA
                     SVL"
     gene            complement(9226..11031)
                     /locus_tag="PKY63_10235"
     CDS             complement(9226..11031)
                     /locus_tag="PKY63_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPF01034.1"
                     /translation="MRSGRIPEIFKVVFILFLLGLSAELFARAGGGGGGDYGGGGGDG
                     GIIEIIIYLLMALPFPYNIIAVGVVILFAVVFKKKARQASVLNKVPTPQSLRDDLKFI
                     KFKQQNPDFNEEFFKKKVNIAFYGIQNAWMAQDVRKVRKFISDGVYQRFNVQFIMMKK
                     LQQHNQLTNINLHSITMADYRRDGQYDVLDVALHASMNDKFVSEKIPRLSSGGYESFV
                     EYWTFIKKSGFTPGDIYENPNCPQCGAALPEIEGETGICPYCKAVVNSGDFDWVLSEI
                     TQADDYTLSHSMGHKQNNLQLKIQQLHSGDPAFCVQKLEDKASNAFLQHTVATALLNP
                     ALARRFMLEDAYQRLKTDIQSYSPILFNRLYLNDVSLISSWAENEYDYLGFFVRKSFQ
                     RIEMKRNNPEEVEAIMDPVVRTMSATIVLCRKRGAQPPRGLLYAHQCPNCGGTLADTT
                     DIKCPYCGSEVNSPAFEWVVDGVYSIGEYKQRIAGNVPDELIAFGVDKADDLMDVRDY
                     AFNNALIVMAADGYFATEEELFAKALAKKFGYNPAKIQPAIELAKNKKLVLRMPDDPK
                     KHAKIIRLMDKAAMADQQMTTAEAELMSYVHSNFS"
BASE COUNT         2934 a         2673 c         2301 g         3311 t
ORIGIN      
        1 caaaaaaagc ggacatcttt cgaagcccgc ctttcccttt acttcagatt gttttataaa
       61 ctgaaagtat tcatttcgcg gataatttct tcgggatcct gcaataattc aggatgcttg
      121 tcgagatgta tcagcgccga aatcggcaac ttggctacat gaaattccgg tccgccatcg
      181 gcatccggag tcttgacaac aagcaatgaa cctgttttca gaattatgtt ttttgcagac
      241 gaagattcaa gtttaaaacg caggattgct tttccaagtt cgacattttt atcgctgtca
      301 ccaatcaaca tgctgtaaat ttcatcgatg cgctcgtgaa cctgcctcat tgacattggt
      361 tctttcgaaa tgcccatgcg ctcgctggct ctttcggctt ttttattggg attgtagaat
      421 gtaaatcctg aggcctcaag aatatcgagc aatgcttcat gcactttgtc aatgctcttt
      481 ttgtcttcgg tatcgataaa gatatttacc ggcaaatggc gcttcagctc acgcttttta
      541 tacgtcgagt tgatggaatt aaacgaaata tattcactga atgcattttg caaaatatag
      601 atctgatctt tcaaacgctg cagttctttc acgtgatttt tttcgagttt gccgattttt
      661 tccttgtaat actcatagta ttgcttcatc atggcgttct gcgggcctgc agcaaccagt
      721 cgtttacctt cgtcgatgtc ggtaaagttc tggaaattct caatgaattt atcggccagc
      781 atttgtgcct gattgtcgta ctgtgcaggg tatttccacg aattgcgcgg gttcaggatt
      841 ttcggatcaa tgtcgctcag ctgttttgga atctgtaaac cgaagaaagg catttcttcc
      901 attggtgctt catcgatgct gccatcaaga atggcatcaa taattttgcg ggtcaccggc
      961 aaatcaatgc gacgtcctgt tccatacgga ccttcgatcc agcccgtgtt tacaagatat
     1021 gcggtagctc cgtgcaaatc catctttttg gccagttcct gcgcataagt tgccggatgc
     1081 aacaacagaa acgctgctcc aaaacaggcc gagaatgttg gctgtggctc tttcacccca
     1141 cgttcggttc ctgccagctt gctggtgaat ccgctcatga aataatacat ggcctggtca
     1201 cgcgtgagtt ttgccaccgg aggcaacaca ccgaaagcat cggcggtgag aaaaataact
     1261 ttcttcggat gcgtgccacg tgaaaccgga gttacgatat tccgaatgtg ataaatcggg
     1321 tacgaaacgc gtgtgttctc ggttttggac tggtctgaaa aattgatttc acctgttttt
     1381 tcatcataaa ccacgttttc cagcaaagca tcgcgcttga tggcattgta gatatcgggt
     1441 tcgtttttct tactcagatt gatgcatttt gcatagcaac cgccttcgaa attgaagacg
     1501 ccatcatcgt cccagccatg ttcatcgtcg ccgatgagaa aacggtccgg atcggccgac
     1561 aatgtggttt ttccggtgcc gctgagtccg aagaaaatgg cggtgtcgcc gtttttgccc
     1621 atattggccg aacaatgcat ggcagccatt ccgcgcaaag gcagaaaata attcatgatg
     1681 ctgaaaaatc cttttttgat ttctccaccg taccacgagc caccaaccac cgacatgcgt
     1741 tcttttatgt tgaatgctac atacacatcg ctgttgagct tatgtttttc ccaatcggga
     1801 ttaaccgttt tgcaggcgtg cagcataacg aaatcaggct taaaattggc cagctcagtt
     1861 tcagccggac gaatgaacat gtttttcaca aaatgtgcag cccatgctac ttcagtaact
     1921 atcctgattt tcaggcgggt gttttcattg gctcccaaat aggcatcctg aacgtacaat
     1981 ttttttccat ccagctgctt ctgactggtt tcgagcaatt ctttccagac tgcttccgaa
     2041 ataggtttgt tgtcgctccc tttgcgacct tcgccggccc accagatgtt gtcggcactt
     2101 tccgattcat gaacaatata tttgtctttc ggcgaacggc cggtaaatac accggtatca
     2161 acattcaccg ctcccagatt ggtaacgaac ccccgttcaa acccttctaa ctttttgtcg
     2221 gtttcgtgct tgaataattc gtcgtaggaa agattgtaat acaattcgcc aaccgaattt
     2281 attccgtatc cggccagctc tttcttgatt tttggcaaca gtttttccat aaggcgcttt
     2341 tttgcaaaat taaaaagtca aaaccacttt attgtaaaaa atgtatcagc tttttacctg
     2401 ttttttaaaa atttgtattt ttgccacttt gacgtaggat gttttgatcg ttttctgtcg
     2461 tttcagactt cagtattcag ctagtcagca ttcagcttcc tgacatttta ccgtcagctt
     2521 gtacaggcca acaatcacac ctgctgacgt ggaatcgtca ggaattgcaa tatttagaga
     2581 aaaatcatcc aaacacccaa cgattccggg tccgcaccac tgcgccgaaa gaagactctc
     2641 ggacgccaaa atgttgggtg cgtatactac gctgggcgcg cgtctctgca tgcaacgttt
     2701 cacgttgacg cgtgacttac cgactcaaag gtgcttcgca cacattgttc ctggggcctt
     2761 tggcccaagg ctaacatatt ttgcgctgga atttactccg ccgaggcgga ggcgcatcct
     2821 atggcacttc gtagctttct tttttgcctt cggcacattg tgcgttgagc catgaaccgc
     2881 atgcccaata cagagtggga atcagagcga gagcgaagct tagccaaact gcatgcctta
     2941 cgcctcattg attttcatga cgcaactcat gcaatcgtat tcgagtctga ataatttttc
     3001 tccttgtgcc aggtgttgcg gatcgagctc cggttgcgcc ttttgatatg tcggacaaca
     3061 tccggtttca taacgtaaac acaatttcgt agtcatcaga gtttgtcctg aatgatcatc
     3121 actcagctca aacgccggct ccgttttcac aacgccatgt cgtttgtaaa accgcgctga
     3181 aagcgcgttt gaaatgttgc tgcggtaatc cgtttcgttt tcgaaataag gagtgtcgtt
     3241 tggaacaatt tgtttttctt cgcgcacata cgccgaaagc agcatctggt caaattcttc
     3301 gaatgcttca cgccggatgc ggttcaactc ccctatcgga aaaaacaacg gactccccca
     3361 cttcaaatcg aatgaagcta catcgtaaaa cgtagatccg gtcttgctta gctgcgtttt
     3421 tatggtgtct gatgctttct gtccgtcgcg cgccggaact ttttcaattg caattttttt
     3481 attcagcgaa taccttccat catcagtgct gatggtcaat tcaaaacctt cgtctgtttc
     3541 cccgaaaatc atgttcaccg gaatcctgcg cgcagagcca gctgcagcca actgccggtt
     3601 aaatgcaaga tcgaggttcc tgtatatttc tgttcctttg gctatattaa tcggatcgtc
     3661 cgaaaatatt tttccattgt cggcatactg aacagcaaag cccttcagct caccgttttc
     3721 atcaaaccag caaaggccat cgccattgga aatttcagga cagttatcta ttttaatgaa
     3781 attccagccc gactcactca cctgccccag ccgctttccg agcgactttg aagtatgtgc
     3841 cgacactttt tcggtgcgtc cgtgcagaaa atattccgtg aatccgcgat tgaaggattt
     3901 ttccggatcg ggctcaaagt taggctggca gcgccccagc gacgatatct tgtatttttc
     3961 gttgtgctca attatttcat ccagcttgcg gcgatagtgt gcagttatgt ttttcacata
     4021 attcatatcc ttcagccggc cttcgatttt gaaagtcgta acgcccgccg ccatcatctc
     4081 tccgaggtgc gcactgcggt tcatgtcgcg cagcgaaagt gcatgtgtgt tggattcaat
     4141 aatatttctt ttgtcatcaa gaatatcgta tttcaggcgg cacggctggg cgcattcgcc
     4201 ccggtttcca ctgcggtgac cgtgaaaggc gctcatatag cattgtccgc tgaaactcac
     4261 acagagcgca ccatgcacaa aagcttcgag ctcaatattt gtctgcgacc tgacttcagc
     4321 tatttgcttc agcgacaatt cgcgggcaag aatcacccgg ctgaaaccaa catcctgcag
     4381 gaattttatc cgacggacat catagttgtg tgtctgcgtg cttgcatgca acgcaatggg
     4441 tggcagatcc atttcgagaa ttcccatatc ctgcactatc agcgcatcgg caccggcatt
     4501 gtaaatatcg tgaatcaact tctcggcttc ggccagttca tcatcataca aaatggtatt
     4561 cagcgccaca tatacttttg catgaaagcg atgcgcatag ttgcacagcg tttcaatgtc
     4621 ggcggttgaa tttcctgcag ccgcgcgggc accgaatttc ggcgcaccga tatacaccgc
     4681 atctgcacca aaattaatgg cggccatacc atgttccgca tcgcgcgcgg gactgagaag
     4741 ttcacacccc tcaaagcgtt ctgaaccctt cgaggggtta cttattagca gagattccat
     4801 catttccccg tcagtttttt aattacctca atcagccggt tttcatcaaa aggttttgtg
     4861 atgtagctat ccatgccaac tttaaggaat ctttcctcat cgcctgaaat ggcgtatccg
     4921 gtgatggcaa tgatgggggt gtgaatgccg ctctgagtct ccttttcgcg aataatccgg
     4981 gtggcttcga aaccatccat ctgcggcatc tggccatcca tcagaataat atcgtatttc
     5041 tctgcgtcgt atttttcaag tgcctgcaga ccattgtagg ccgaatcgac aatcattccc
     5101 ttgctgctca gaaaaccttt cagatacatc tgattcagtg catcatcttc aaccagcaaa
     5161 actttgcatt cgctttgaac gggctcatcc gtaacgatat tcgtttcttc ggcatcgctc
     5221 aaaggcgcat tgaacggaat tgtaagaata aagcggctgc cgtgtccaaa ctcactttca
     5281 actttgatgt cgccgcccat catttcggca aaacttttta caatggccag tccaagcccg
     5341 gttcctgaga attttttggt ggtggaactg tcaagttgag taaacgaatg gaagagtttt
     5401 ccaaaatcct cttttttgat tccgattccg gtatctgaca cttcggtggt gattatagtt
     5461 tgaccatctt cggctgtcga cgccgaaaca tgcagtataa cagaaccctt gtcggtgaac
     5521 ttcactgcat ttccggtcag gttaataacc atttgtctga accggtttcc atcgccccgc
     5581 agacgggcag gaacagaagc atctttgcgt atttcgaact taatgccctt gccttcggcc
     5641 accgatttgt acacagaact gacctcaccg aaaacggcat ttacattaaa gtccttgtct
     5701 tcgagttcaa tgcggtttgc ttcgatttta gaaagatcga gaacatcgtt tataatatgc
     5761 aacagattgt cggatgaagt gagtatggct tctacaattt cctgaacatc tttgttgaaa
     5821 ttggttctgg acagcgtttt agccataccg acgatggaat tcagcggatt acggatttcg
     5881 tgactgacat tggcgagaaa ctccgatttg gctttattgg cggcttcggc aaattctttt
     5941 tcttgaataa gttgctctgt tttaactttt tcgctgatat cgcggcaaac agcaacaatc
     6001 tcatttttcc cgctaatagc atttttcaca agctttaccg atgattcata ccaaacaaac
     6061 gaaccatctt tcatcatcac acgatgtttt aacaaaggca aatcacaacc tgacaaaata
     6121 tgtttctgcg actccagaat agaaggcaaa tcatcaggat gaataaaatc aaaaaaagta
     6181 tgttgaataa catcttcagc atcaaatttc ttaattctgc cgctgccggg cgataaatat
     6241 gaaataatac cactttcagc gtcaaaagca acaatcatgt cgtttgaatt ctccgtaatc
     6301 aatctgaatt gttcacggct ttcaagcaat tccttcatca ccctttgacg cgcaatatgc
     6361 gaccacatgg ccgatccgag aatggtcagc tgttttatgc attcgtcgta gtattcatct
     6421 tccttgtttc caacgccaag cactgcaaca atcctgtcat tatcaaaaac cggaacagat
     6481 aaatgtcttt tgagtgtaac atggccttcg ggatatcctt ttttccacgg attatcagct
     6541 gaataatcat tggtgattac aggtttccgc tgacgcacgg attcgcccca gagcccggtt
     6601 ttatcgacca gatattcgcg tggagcatca cttacattgc attcctccat ggcgcttttc
     6661 gaccaggcat gcatggtgag gacatcctct gtttcattca aaaaggcaag ataaccgatt
     6721 ttacttccag tcaggctgat aacttcctca aggacaaaat tagtaatctc gttcaatgga
     6781 gcattaatca tttcactgag ctgaagcaaa gccatggtgc ggcgtgcatt tatattctgc
     6841 atctgttcag ctttcttacg ctcagtgata tcggtcagag acgttatccg aagcttgcgt
     6901 ccatcgctga attcggcatg ccttggcatg atttcaacag gaattattgt accatcgcgg
     6961 cgaattattt cagcttcgta tttatcggtt ttttcaccaa gcacattatt cagtactttt
     7021 tctttgtaag ccggcgaaat ataatcgatg atgtttttac cgacaagttc cttttcatca
     7081 gtgtaacccg acatttcgag catggcaatg tttacatcta ccacaatgcc tttatcatga
     7141 aaaatcaatc cttcctgaga gcattcgaaa aacatccgca gccggctctc gcttttattg
     7201 agagcttcct gcgccagaat gctttcggta atatccttgg caaaacactg gtaggtacca
     7261 tccggcatta agcgcgaact catttcgatg tgaaccagat tccctttttt gtgttttaat
     7321 aaacgtcgct tggtcaatgt ttttcccgtc agcaaaatat catagcgcaa aggttctgca
     7381 tccagaacat tggacgtgaa aagatctttc atgttcagct tcagtaactc ttcgcgtgaa
     7441 taaccggtta actcgatggt ggcctcgttt acatccataa actgtccatt cgaatcacca
     7501 tgaaaaaaag cttcgggcgc cagcgccaat actgttcgta ttttatgctc acttttgcta
     7561 agcgcctctg ttatcttttg tcttttgtcg aattcttgtt ccaaacgtgc tttttctgtc
     7621 cggatgacaa ggttgttata acccgattct gaaatatatg tagcaatctg ccccaaaaag
     7681 gacgttgcaa gcaataaatc tttttcactg ataactttaa cctcttcaaa agccctgata
     7741 tatttttctt cgttgaagcc atattttttt gcctgagctc tgaaaaaaga attgtcgggc
     7801 ttttccaaaa gcaattgtcc ggttgtgata caagcaatca gtttatcctg aataaatatg
     7861 ggcgtagcac aatcgtacat gccgtttccg catttttcta tcaccaactg cccttgtctt
     7921 tccaaacgat caaacagctt gcggttgcaa atcaagcaat gctttgctgc ttcgggataa
     7981 gccatgtgaa aatccgtgca aacttttttc caaccggttt taaatagaat agtttgatcc
     8041 gggtattcaa ataaacctat cgttaacccg gttatttctg taaatttttc cgataattcc
     8101 ttcaacatac caatatctac caaatcggtc agcttgaatg aattgttttc taatggattt
     8161 agttcgtttg cattggattg cataaaaata aattatgctg caaagatacc atttcacgaa
     8221 gctatatcca acaactctga ttatttttgg taataattta aaagtgtccc tgttttttca
     8281 tcaagtaaaa agatgctata tttgaacttt atatactgat ttttatgaaa tggcaaggca
     8341 gacaaggaag ctcaaatgtt gaggacaaac gcggcaaatc aaaaggtttg ctgggcggtg
     8401 gcattggcac tataataatt attattgtta tttatctgct tggtggcgat ccaagcggta
     8461 ttatgcaaaa tgcaacagat ccgtcgaatc aaactaccga agaatacaat ccatcggcaa
     8521 aagaacagga actggccgaa ttttcatcgg ttgttttaaa agaaaccgaa gatacctgga
     8581 caaaattatt taatgaacaa ggcaaatcat accggtgtcc gaaactggtt ttgttttccg
     8641 aagcagttca atcgggatgt ggcgcagcca gtgccaattc aggccctttc tactgtccgg
     8701 ccgatgaaaa agtatatatt aatctgcaat ttttcgagga actgggatcg cggtatggtg
     8761 ctcccggcga ctttgccatg gcctatgtaa ttgcgcacga agtgggccac catgttcagc
     8821 ttttgcttgg catcaatgag aaatatgaat catatcgcaa ccggctcagt gagaccgaat
     8881 tcaacaaaat gaccgttcgg ctcgaactgc aggccgatta ttttgcaggg gtatgggctc
     8941 attatgccga acgcatgaat ctgctcgaag aaggcgacct cgaagaagcg ctcactgccg
     9001 ccagtgccgt aggcgacgac cgcctgcaga aacaggccca gggtcatgta aatcccgaca
     9061 cttttacaca cggaacctcg gaacagcgca gccgctggtt tatgaaaggt ttccgctccg
     9121 gcgatatgta cggcggcgac acatttgccg caagcgtact ataacatcgc cagctatgcg
     9181 aagtatatac ttcgcatagc ggcaaacttt tcatagctgt tccagctatg aaaagtttga
     9241 atgcacatag ctcatcaact ccgcctcggc ggtagtcatc tgctgatcgg ccatagctgc
     9301 cttatccatc agacgaatga tttttgcatg tttcttcgga tcatcaggca tacgaagcac
     9361 cagttttttg ttttttgcca gttcaatagc cggttgaatt tttgcaggat tgtaaccaaa
     9421 ttttttcgcc aatgctttgg caaataattc ttcttcagtc gcaaaataac catccgctgc
     9481 catcacaatc aaggcattat tgaaggcata atcgcgcaca tccatcagat catcggcttt
     9541 gtcaacacca aatgcaatca actcatcggg cacattgccg gcaatgcgct gcttatattc
     9601 tccgatggaa taaacaccat ctacaaccca ttcaaaagca ggactgttca cttcagaacc
     9661 acaatacgga cattttatat cggttgtatc ggcaagtgtt ccaccgcaat tcggacactg
     9721 atgggcatac aacaatccgc gcggtggctg agctccgcgc tttcggcaca aaacaattgt
     9781 agcagacatt gtgcgaacca ccggatccat aatggcttcc acttcttcag gattatttcg
     9841 cttcatttca atgcgctgaa agctttttcg gacaaagaaa ccgagataat catattcgtt
     9901 ttcggcccat gatgaaatta aacttacatc gttgagataa agccggttga ataatattgg
     9961 tgaatacgat tgaatatccg tttttagccg ctgatatgca tcttcaagca taaatcgtcg
    10021 cgccaatgcc gggttgagca atgccgtggc aacagtatgt tgcagaaacg cgttactggc
    10081 cttgtcttcg agcttctgca cacaaaaagc cggatcgccc gaatgtaact gttggatttt
    10141 caattgcagg ttgttctgct tgtgccccat cgaatgactg agtgtataat catctgcttg
    10201 tgtgatttca ctcagcaccc agtcaaaatc accgctattc acaaccgctt tgcaataagg
    10261 acaaatgccg gtctcacctt caatttccgg caaagcagcg ccacactgag gacagtttgg
    10321 attttcgtaa atatcgcccg gcgtgaaacc cgattttttt ataaaagtcc agtattctac
    10381 aaacgattca taaccacccg atgacaaccg cggaattttt tcagacacaa atttatcatt
    10441 catgctggca tgcagtgcca catcaagcac atcgtactgc ccgtcgcgcc gataatcggc
    10501 catggtgatt gaatgcagat taatattcgt taactgattg tgctgttgca gttttttcat
    10561 catgatgaac tgcacattga aacgctggta aacgccatcg ctgatgaact tgcgtacttt
    10621 gcgcacatcc tgagccatcc aggcattctg aataccataa aaagcaatgt taaccttctt
    10681 tttgaagaat tcttcgttga aatcagggtt ctgctgctta aatttaatga atttcaaatc
    10741 gtccctcaaa gattgaggcg ttggaacctt attgaggacc gatgcttgtc tggctttttt
    10801 cttaaataca acagcaaaca aaattacaac tccgacagca atgatgttgt atggaaacgg
    10861 aagggccatt aaaagataaa tgatgatttc aataattcca ccatcaccac cgccaccgcc
    10921 ataatcgccg ccaccaccgc caccggcacg ggcaaagagt tcagccgata ggcctaagag
    10981 aaataagata aaaactactt tgaaaatttc tggaattctg cctgatctca tgaaatattt
    11041 ctttggtgta aatgtataaa gaaaatcatt ttcagaatca cttggggtca tggaatttcg
    11101 aatgccttat tagcattgta ttccggtgtt catatttcaa tgacaattca atcatatttt
    11161 gttatacttt tgcgcttcaa attcaaacaa aactctatgg aaaatcagaa acaagccaa
//
LOCUS       DAOPKE010000089        10985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6253_length_10985_cov_12.740165, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000089 DAOPKE010000000
VERSION     DAOPKE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_10985_cov_12.740165"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..467
                     /locus_tag="PKY63_10240"
     CDS             102..467
                     /locus_tag="PKY63_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01035.1"
                     /translation="MSDKLCLECGEPLLGRADKKFCSDQCRNTFNNRMNSDSINLVRN
                     INNQLRKNRRILTELNPEGKTKVHKDKLLSKGFSFKYSTHSYTTQKGTTYFFIYDQGY
                     LALEDGFYFLVVDKRLLDK"
     gene            473..1825
                     /locus_tag="PKY63_10245"
     CDS             473..1825
                     /locus_tag="PKY63_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPF01036.1"
                     /translation="MRIHFIAIGGAAMHQLAIALHLNGNVVSGSDDEIFDPALSNLRR
                     HHLIGDHYSWDPDSVTENIDLIILGMHARSDNPELLKALQLGLDVVSFPEFVFRSSQN
                     KKRVVIGGSHGKTTITSMIMHVLKECGRPFDYLVGSQVRGFDVMVSLNEKNDLIVIEG
                     DEYLSSCLDPRPKFHHYHPDIAVVSGIEWDHINVFPEFEQYTGAFRTFINAVPDHGSI
                     IWCANDMNVSKLMSDYQGAAERVSYSAIPHFLENNRLSVLCGLVPVPVNVFGEHNMLN
                     MSAALEVCRRIGISDESFFSAISGFQGAARRLETIYDEDGLRIFRDFAHAPSKLKATV
                     NAVKSRFADEQLVAVFELHTFSSMNRKFLPHYAGALDQANVRAVYFSPHAFKLKKLEP
                     FTEAEIREGFQNTNLSILNSPEALIGFIRQQVTKPANILLMSSGNFENANFDEIPLLN
                     "
     gene            1870..2952
                     /locus_tag="PKY63_10250"
     CDS             1870..2952
                     /locus_tag="PKY63_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPF01037.1"
                     /translation="MKNRALIITSCLMLISVLTFAQAKPTIQFQSKVYDYGKIKEADG
                     VAKGVFTFTNIGNDTLRITAVRPSCGCTASSYTKDPIPPGGTGSIEAGYNAAGRPGHF
                     NKAITVTTNDPDNATIVLSIKGEVIPKPKSKADEYPNKIGNLYFKTNHLAFQDLKENE
                     IKTDTFKLYNNGTKELTIKFNELPAFLKAEIKDPVIKPDMESTILITYDAKARADYGY
                     VFDKFFLTTDDVDQPEKLLYVSANIVQDFSWMTEKNKKKAPHILFEAESHDFGSVKVG
                     TNVEYSFKFKNVGKSTLKILKTKASCGCTATDPVKTVLKKGESSEIKVVFNTTGRKGN
                     QHKTITVITNDPDKSVIVLHIQGNVE"
     gene            complement(3012..5513)
                     /locus_tag="PKY63_10255"
     CDS             complement(3012..5513)
                     /locus_tag="PKY63_10255"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPF01038.1"
                     /translation="MKSVQFSIDEIVDICKAELLQSNAKNIRISGIITDSRHISDGKN
                     ELFIALKGVRHNGHLFLKEAYNKGIRTFIISDISADTELPADATILKTNNTLHALQSL
                     AAAHRKKFNIPVIAITGSNGKTMVKEWLYQLLSNDTDVVRSPKSYNSQVGVPLSVFNL
                     EANHRLAIFEAGISQPGEMQSLENIIQPNIGILTNIGAAHDENFIDHRQKAAEKLQLF
                     VNCNKLIYCSDDFEIEQKIISSGLNKKVEMISWGRKNDAWLQITDVIANKGGTNISAV
                     RKDETLRIFIPFTDKASVENIIHCWVYLLSENMAQNLISERIRKLSSIEMRLELKEGK
                     NRCSIINDSYNSDINSLSIAIDFLEKNKEYTRKTIILSDILESGRNSKDLSNEIAKLI
                     AKRGIDRFIGIGENLCNNSKTFSGESYFFKNTEDFLHGFDLSIFRDEMILLKGARRFG
                     FERISALIEDKAHETILETDLNALTHNINYFKSKLSSGTRMMAMVKAFAYGSGAIEIA
                     RHLEYHRFDYLAVAYADEGVALRNAGITLPIMVMSPEENNYYFLFRHQLEPEIYNFAT
                     LQKLIENIELYSSFIALPVRIHIKFDSGMHRLGFTENDAEELFSILNENADCIQIASV
                     FSHFTSSDDVTENAFTELQLKRFKEFAGNLEKSIGYKTLKHISNSAAILRLPQAHLDM
                     VRLGIGMYGIDPAGIHNNELQPVVSLRTKILQIKHIAAGETVGYSRKAKSEHARTIAT
                     LAIGYADGFHRNMGNGLWNVFINGQAAPIIGNICMDMCLADVSHINNIKEGDEAEIFG
                     RHNSLISYAETMGTIAYEALTSVSARVKRIYMQKD"
     gene            complement(5522..6547)
                     /gene="pdxA"
                     /locus_tag="PKY63_10260"
     CDS             complement(5522..6547)
                     /gene="pdxA"
                     /locus_tag="PKY63_10260"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPF01039.1"
                     /translation="MKEREHKKVVAAITHGDVNGIGYEVILKTLADSRMLDFCIPVIY
                     GNSKAASYHRKTLSISDYQLTLVRDLTQLKAGKPYIVNIINEEIKIEFGKSDPVAGQL
                     AHKALEMAITDINAGQADILVTAPINKSNIQSPTYHFPGHTEYLAEKCKTDDYMMLMI
                     SPNLRIGVVTGHVPVNKISSELSISLIEKKLAILNHSLKYDFGVSNPKIAVLGLNPHA
                     GDDALIGDEEKNIIEPAIRKAFENHINAFGPFPSDGFFGNTAFRNFDAVLAMYHDQAM
                     IPFKVMAFEDGVNFTAGLPIVRTSPDHGTGYDIAGKNMASCNSFRNALILGVEIYYNR
                     LNYNKNK"
     gene            complement(6596..7204)
                     /locus_tag="PKY63_10265"
     CDS             complement(6596..7204)
                     /locus_tag="PKY63_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPF01040.1"
                     /translation="MLKQLRYIAFLFFPALCGAQEIPDMTPPGISTAIVYTDTNQIAS
                     NTDAVEYIDLSRQKLTSIPAVVFQCPNLIYLDLKKNKLENIPAEIGQLNKLRVLNISR
                     NRLVRLPKEIGQLSALKSLILNQNEIIELPPEIGMLSNLAVLDLWGNQLDKLPKEIGK
                     LSETLQYLDMRVIFMSREAQEVIEIMLPETKIFFSQSCNCKD"
     gene            complement(7198..8136)
                     /locus_tag="PKY63_10270"
     CDS             complement(7198..8136)
                     /locus_tag="PKY63_10270"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPF01041.1"
                     /translation="MQTIYSNHNFNQPDEGTCITVGTFDGVHLGHAEILRFLKSVSKN
                     HQLASIVFTFGTHPRYVLKSDAGELKMLTTLNEKAHLISLFDIDFLYVQEFTPDFSSI
                     SAEKFISDILIGKLNMKSLVVGHDHLFGKDRSGNFALLSELSDKYGFSLIQIPAISSS
                     GFNISSTKIRNLLAASDVQMANDMLGYHYSFTGEVIKGFGIGRELGFPTANMRLSDSH
                     KILPPPGVYIIKAKHASDMYEGIINIGIRPTFEGNIVQIEAHLFDFDKHVYGELLTIE
                     FLLFLREEIKFNSREELISHIRKDRDAAFKYFGRIC"
     gene            8384..10810
                     /locus_tag="PKY63_10275"
     CDS             8384..10810
                     /locus_tag="PKY63_10275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPF01042.1"
                     /translation="MRKAFAILTALLAAPMLSLAGEADLVVPEGMKDSTILYWGFLIT
                     LAGMLFGLYQFIKVRKLRAHRSMLDVAQVIFETSKTYLLRQGRFLVILFIFIGAAVGF
                     YFGFLSEGFGLGGVLMILGWTVVGILGSYAVAWFGIRMNTLANSRMAFASLERKPLKL
                     LNIPLNAGMSIGVMLISLELTIMLIILMFVPGQYAGASFIGFAIGESLGASVLRIAGG
                     IFTKIADIGSDLMKVIFKIGEDDPRNPGTIADCVGDNAGDSVGPTADGFETYGVTGVA
                     LVSFILLAVGGPLFNMDAAATQDLQIKLLTWIFVMRVLMIITSVVSFWINGFISRARY
                     HNADDLDFEKPLTSLVWITSIISILVTFAVSYVIIGDLPHDMWITLSIIISAGTLGAA
                     LIPEFTKIFTSPKSAHVQEVVESSRRGGASLNILSGLVAGNFSAFWQGLVFVVLMAIA
                     YFASLYGLGEVMVYPSIFAFGLVAFGMLGMGPVTIAVDSYGPVTDNAQSIYELSLIEE
                     VPNVKEEIEKDFGFTPDFEKSKHYLELNDGAGNTFKATAKPVLIGTAVVGATTMIFSL
                     ILVIQESLGVNPEQILNILNPYTILGFILGGAVVFWFSGASIQAVTTGAGRAVEFIKK
                     NINLDPNAPKKADTEKSKQVVAICTNYAQKGMFNIFIVIFSFALGIAFLSSPIDGAVD
                     PNAPVAMFVSYLISIALFGLFQAVFMANAGGAWDNAKKVIEVDLKEKGTDLHAAAVVG
                     DTVGDPFKDTSSVALNPIIKFTTLFGLLAMEIAISEAFRGVSKYVGAIFIIIALIFVY
                     RSFFSMRVDK"
BASE COUNT         2939 a         2159 c         2403 g         3484 t
ORIGIN      
        1 tactttaacc attttgcgtt aaataaaaaa atatttacta cttttagggg ccgtttttta
       61 tctgaacggt cctttttttt gtttgataaa atcctatagt tatgagtgat aaattgtgtc
      121 tggaatgcgg tgagccgctg ctgggaagag ctgacaagaa gttctgcagc gatcagtgcc
      181 gaaacacttt caacaaccgg atgaatagtg actcgattaa tctggtgcga aatatcaata
      241 atcaactaag aaaaaaccgg agaattctga ctgaacttaa tccggaaggc aaaacaaaag
      301 tgcataagga taaattgctt tctaaaggat tttcctttaa atacagtact catagttata
      361 caacccagaa aggcacaacc tattttttta tttacgatca gggctatctt gcacttgagg
      421 atgggtttta ttttctggtg gttgacaagc gtttacttga taaataaatt tcatgagaat
      481 tcattttatt gctattggcg gcgctgctat gcaccagctt gccattgcgc tgcatctaaa
      541 cgggaatgtc gtttctggct ccgacgatga aatttttgat ccggctttat ccaatcttcg
      601 gcgacatcat ttaatcggag atcattattc atgggatccg gattctgtta ccgaaaatat
      661 tgacctgatt attcttggaa tgcatgccag aagtgataat cctgagcttc tgaaagcatt
      721 gcagcttgga cttgatgtcg tttcttttcc tgaattcgtt ttcagatcca gccaaaataa
      781 aaagcgtgta gtaatagggg gttcgcatgg taaaacaacc attacttcaa tgattatgca
      841 tgtattgaaa gaatgcgggc gccctttcga ttatcttgtt ggcagccagg tgcgcggctt
      901 tgatgtgatg gtttcgctta atgaaaaaaa cgatcttatt gttattgaag gcgatgagta
      961 tctttccagt tgcctcgatc cgcgaccaaa gtttcatcac tatcatcccg atattgcggt
     1021 tgtgagcggt attgaatggg atcatatcaa tgtttttcca gagtttgaac aatataccgg
     1081 tgctttcaga acttttatca atgccgttcc tgatcatggc agtataatct ggtgtgccaa
     1141 cgatatgaat gtctctaagt tgatgtcgga ttatcaggga gcagctgaac gtgtttcata
     1201 ttccgctatt ccgcattttc ttgagaataa ccggctaagt gttttatgcg gtcttgttcc
     1261 tgttccggtc aatgtttttg gtgagcataa tatgcttaat atgtcggccg cgcttgaagt
     1321 atgccggcgc attggtatca gcgatgaatc gtttttctcg gctatttctg gttttcaggg
     1381 tgctgcccgg cgattggaaa ccatttatga tgaagatggt ttgcgcattt ttcgcgattt
     1441 tgcacatgcg cccagtaaac tgaaagctac tgtaaatgcc gtgaaatcgc gttttgccga
     1501 tgaacagctt gttgcggttt tcgaattgca taccttcagc agcatgaaca gaaagttcct
     1561 tccgcattat gccggagcac ttgatcaggc caatgtcaga gcggtgtatt tttctccaca
     1621 tgcttttaag ttgaaaaaac tggaaccttt taccgaagct gaaatacgcg aaggattcca
     1681 aaatacaaac ctgtcaatcc taaacagtcc tgaagctttg attggattta tcaggcaaca
     1741 ggttacaaaa ccggcaaaca tattgctcat gagctctggc aatttcgaga atgcgaattt
     1801 tgatgaaatt cctttgctga actaacaaaa aaaattaact ttgtaaaaaa ttaaaggaga
     1861 atacgaaaaa tgaaaaatcg tgctttgatt attacatctt gtctgatgct aatatcagta
     1921 ttgacttttg ctcaggcaaa gccaaccatt cagtttcaga gtaaagtgta cgattacgga
     1981 aaaattaagg aggcggatgg tgttgctaaa ggtgttttca cttttaccaa tatcggaaat
     2041 gataccctgc ggattaccgc tgttcgcccg agttgcggct gtacggcttc gtcttatacc
     2101 aaagatccta ttcctcccgg aggaacagga agcattgaag ctggttataa tgctgccgga
     2161 cgccccggac atttcaataa ggccataacg gtaactacca acgatcccga caatgctacc
     2221 attgtccttt caatcaaggg tgaagtgatc ccgaaaccga aatcaaaagc cgacgaatat
     2281 cccaataaaa taggaaatct ttatttcaaa accaatcacc tggcttttca ggacctgaaa
     2341 gaaaatgaaa tcaagaccga tacgttcaaa ttgtacaaca acggtacaaa agaactcacc
     2401 attaaattca atgagcttcc cgctttcctg aaagccgaaa ttaaggatcc tgtcattaaa
     2461 ccggacatgg aaagcaccat cctgattact tacgacgcaa aagcccgtgc tgattacggt
     2521 tatgttttcg acaagttttt ccttaccaca gacgatgttg atcaacctga aaagctcctt
     2581 tatgtaagtg ccaatattgt tcaggatttc tcctggatga cagaaaaaaa caagaaaaaa
     2641 gcaccccata tcctttttga agccgaatcg cacgattttg gttctgttaa ggttggaacc
     2701 aatgttgagt atagttttaa atttaaaaat gttggcaaat caacgttgaa aattctgaaa
     2761 accaaggcaa gctgcggatg tacagccact gatccggtta aaaccgttct gaaaaaaggt
     2821 gaatccagcg aaattaaagt tgttttcaat actaccggaa gaaaaggaaa tcagcacaaa
     2881 accattactg ttattaccaa cgatcccgat aagtcagtca ttgtactgca tattcaggga
     2941 aatgttgaat aacacatctt ctgcaattgt aagacccgcc aatgtgcggg tttttttgtg
     3001 cttataatct ttcagtcttt ttgcatgtaa atccgcttta cacgcgccga aacagatgta
     3061 agtgcttcgt atgcaatagt ccccattgtt tcggcataag aaatcagtga attgtgccgg
     3121 ccgaaaattt ctgcttcgtc accttccttt atattgttga tatgacttac atcggccaga
     3181 cacatgtcca tgcagatatt gccaataatg ggtgcggctt gcccgttgat aaagacattc
     3241 cataacccgt tgcccatgtt gcgatgaaat ccgtcggcat aaccaatggc taaagttgct
     3301 attgtccttg catgttccga cttcgctttt cttgaatatc caacggtttc gccagcagca
     3361 atatgtttaa tctgcagaat ttttgtccgc agcgatacta ccggttgcag ctcgttgttg
     3421 tgaatacctg ccgggtctat gccatacatt ccgattccca gccggaccat atcgaggtgt
     3481 gcttgcggca atcgcaatat ggcggccgaa tttgaaatat gtttgagtgt tttatatccg
     3541 atggattttt cgagatttcc ggcaaattct ttaaaccgtt tgagttgtaa ttccgtgaaa
     3601 gcgttttctg tgacgtcgtc cgacgaagta aaatgcgaaa agaccgatgc gatctgaatg
     3661 caatcggcgt tttcgttgag tattgaaaac aattcttcag catcattttc tgtaaatccc
     3721 agccgatgca taccagagtc aaatttgata tgaatccgca ccggaagcgc gatgaatgat
     3781 gaatataatt ctatgttttc aatcagtttt tgcagggtgg caaaattata aatctcaggt
     3841 tcaagctgat gacggaaaag aaagtaataa ttgttttctt cagggctcat taccatgatt
     3901 gggagtgtga tcccagcatt gcgtaatgca acaccttcgt cggcgtaagc cactgcaaga
     3961 taatcgaagc gatgatattc gaggtgacgt gcaatttcaa tggcgccgct tccatatgca
     4021 aacgccttaa ccattgccat catgcgggtt ccgctgctta atttgctttt gaaataattg
     4081 atgttgtggg ttagtgcatt caggtctgtt tcgagaatgg tttcatgtgc tttgtcttca
     4141 atcagtgcgc tgatgcgttc aaaaccaaat ctgcgtgcgc cttttagcaa aatcatctcg
     4201 tcacggaata tgctcaggtc aaaaccatgt aaaaaatctt cggtgttttt gaaaaaatag
     4261 gattcacctg aaaatgtttt gctgttgttg caaaggtttt ctccaatacc gatgaaacgg
     4321 tctatgcctc gttttgcaat gagcttggca atttcgttgc tcagatcttt gctgttgcga
     4381 ccgctttcca gaatatcgga caggataatg gttttacggg tatattcctt gttcttttca
     4441 agaaaatcaa tggcaattga caatgaattg atgtctgaat tgtaactgtc gttaataatg
     4501 gagcagcggt tttttccttc tttcagttca agtcgcattt caatggacga cagtttcctg
     4561 atgcgttcag aaatgagatt ctgcgccatg ttttcagaca gcagatacac ccagcaatga
     4621 ataatgtttt cgacagaggc cttatcggta aaagggataa aaatccgaag tgtttcatct
     4681 tttctgactg cgctgatatt tgtaccacct ttgttcgcaa taacatctgt aatctgcagc
     4741 caggcatcat ttttacgacc ccacgaaatc atttcaactt ttttgttcag cccgctgctg
     4801 ataatctttt gctcaatttc aaagtcgtcg gagcaataaa tcagtttatt gcaattaacg
     4861 aaaagctgaa gtttttctgc cgctttctga cggtggtcaa tgaaattttc gtcatgtgca
     4921 gcgcctatat ttgtcagaat tccaatgttt ggttgaatga tgttttcaag cgattgcatt
     4981 tcgcctggtt gcgaaatacc ggcttcgaaa atagccagtc ggtggttggc ttccagattg
     5041 aaaaccgaca aaggaactcc aacctgactg ttgtaacttt tgggacttcg tactacatct
     5101 gtgtcgtttg aaagcagctg gtagagccat tcttttacca ttgtttttcc attacttccg
     5161 gtaatggcaa tgacaggaat gttgaatttt tttctatggg ccgcagccag cgactgaaga
     5221 gcatgcagcg tgttgtttgt ttttaaaata gttgcatcag ccggcagttc agtatctgca
     5281 gaaatgtctg atattataaa tgttcggatt cctttgttat aggcctcttt taaaaacaaa
     5341 tgaccattgt gccgaacacc tttcaatgcg atgaacagtt cgtttttccc atctgaaata
     5401 tgtcggctgt cggttattat tcctgaaatc cggatatttt tggcattgct ttgcaaaagt
     5461 tctgctttgc aaatgtcaac aatttcatca atggaaaatt gaacggattt catcacggaa
     5521 tttacttatt cttgttgtaa ttcagtcggt tgtagtaaat ttcaacgcca agaataagag
     5581 cattccggaa tgaattgcaa gaggccatgt ttttgccggc aatatcataa ccggttccgt
     5641 gatcgggcga ggtgcgcaca atcggtaatc cggcagtaaa attcacacca tcttcgaatg
     5701 ccattacttt aaaaggaatc atggcctggt cgtggtacat ggccaaaacg gcatcgaaat
     5761 tcctgaaagc ggtgttaccg aaaaatccat cggaaggaaa aggtccgaat gcattgatgt
     5821 gattttcgaa tgctttgcgg atggcgggct caataatgtt tttttcttca tcaccgatga
     5881 gcgcatcatc gcctgcatga ggattgaggc caagcacagc aattttggga tttgataccc
     5941 caaagtcata tttcaatgaa tgattcagaa tggcaagctt tttctcaatc agggaaatgg
     6001 ataattccga agagatttta ttcaccggaa catgtccggt aacaacacca atcctaagat
     6061 tgggacttat catcaacatc atgtaatcat ctgttttgca tttttcggcc agatattccg
     6121 tgtgcccggg aaaatgatag gttggagatt gaatgttgct tttgttgatg ggagctgtta
     6181 ccagaatatc tgcctgacca gcattaatat ctgtaatggc catttcaagc gccttgtggg
     6241 cgagttgacc tgcaacagga tcggactttc cgaattcaat ttttatttct tcgttgatga
     6301 tgtttacaat gtagggcttc ccagctttca gttgagtaag gtcgcgtacc agtgtgagct
     6361 ggtaatcgct gatcgaaagt gttttacgat gatacgaagc cgccttcgaa ttaccataaa
     6421 tgacaggaat gcagaaatca agcatgcgac tgtcggccag tgttttcagt atcacttcgt
     6481 aaccgatgcc gtttacatcg ccatgagtga tggcggcaac aacttttttg tgttcacgtt
     6541 ctttcatgtt cagaaaaata ggaaaacaaa ggtactcaaa aatcttcgga tggttttaat
     6601 ctttgcaatt gcacgactgt gagaagaaaa tttttgtttc aggcagcatt atttctatca
     6661 cttcctgcgc ttcacgcgac atgaatataa ctctcatgtc gagatattga agcgtttcgc
     6721 tgagcttacc tatttctttg ggcaatttgt cgagctggtt tccccataaa tcaagtaccg
     6781 caaggttact gagcatgcca atttcgggtg gcagctcaat gatttcgttt tgattaagaa
     6841 tcaatgattt caatgcactg agttgcccta tttctttagg gagtctgact aatcggttac
     6901 gtgaaatatt caatactctc aatttgttta attgccctat ttcagctgga atgttttcca
     6961 gtttgttttt tttcaggtcg agataaatca gattggggca ttgaaaaaca accgctggaa
     7021 tggatgtgag tttttgcctg gataaatcga tatattctac tgcatccgtg ttgctggcta
     7081 tctggttggt gtcggtgtaa acaatggctg ttgaaattcc gggcggtgtc atatcaggaa
     7141 tttcctgtgc gccgcatagg gcgggaaaaa acaaaaaagc aatatagcgt agttgtttca
     7201 gcatattctt ccgaaatact taaacgcagc atcacggtct tttcgtatat gagagataag
     7261 ttcttcacgg gagttgaatt taatttcttc gcgcagaaac aataaaaatt ctatcgtgag
     7321 caattcccca taaacatgtt tgtcgaaatc gaaaagatga gcttcgatct gaacaatatt
     7381 gccttcaaag gtaggtctga tgccgatgtt gatgatgcct tcgtacatat cggatgcatg
     7441 tttggcttta atgatataaa cacccggcgg aggcaatatt ttatggctgt cggataagcg
     7501 catatttgct gtcgggaagc caagttcacg tccgatacca aaacctttta tcacttctcc
     7561 ggtgaaggaa tagtggtacc cgagcatatc attggccatc tgaacatcgg acgctgccag
     7621 cagattgcgg attttggttg aactgatgtt gaaacccgaa gagcttattg ccggaatctg
     7681 aatcagtgag aaaccatatt tgtcgctgag ttccgacagc agggcaaagt tgccactgcg
     7741 gtcttttcca aacagatggt cgtgccctac aaccagactt ttcatgttta atttgccaat
     7801 gagaatatca gaaataaact tttcggcaga tatggacgaa aaatctggag tgaattcctg
     7861 aacatataga aaatcaatat cgaaaagact gatcagatgc gctttttcat taagtgttgt
     7921 aagcattttc aactcgccgg catcggattt caacacatat cgcggatgcg ttccaaatgt
     7981 gaaaacgatg gaagccagtt gatgattttt ggaaacagat ttcagaaagc gaagtatttc
     8041 agcatggccc agatgcacgc cgtcgaaagt accgactgtg atgcaggttc cttcgtccgg
     8101 ctgattgaaa ttgtgattgc tgtaaattgt ttgcataaat gagaatgagt gacaaagata
     8161 ctaattgaaa aatgaaattg gatttattca aaatgaaaat ttcccatgaa gataaatcag
     8221 gcagcgaaag tttttttgtc cttttatggc ttaaaaacgt cagacacagg gttttaagcg
     8281 ctgaacatat tgaatattta tgccatttgt catattatac gattatattg aataatgtag
     8341 tacttttgca aaaaatttca atcaccaaaa aaattatgtg tctatgagaa aagcgtttgc
     8401 cattcttaca gctcttttag ctgcaccaat gctttcgttg gcgggggaag ccgacctggt
     8461 cgttcctgag ggaatgaaag attcaaccat tttgtactgg ggctttctga tcaccctggc
     8521 cggtatgtta ttcggattgt atcagttcat caaagttcgt aaactgcgcg cacaccgctc
     8581 catgcttgat gttgctcagg ttatttttga aaccagcaaa acctatcttc ttcgtcaggg
     8641 acgattcctt gtcattctgt tcatttttat tggcgctgcg gttggtttct atttcggttt
     8701 tctttccgaa ggttttggat tgggtggcgt gttgatgatt cttggctgga ccgttgtcgg
     8761 tatccttggt tcatatgctg ttgcatggtt tggaatccgg atgaataccc tggccaatag
     8821 ccgtatggct tttgcttcgc ttgagcgaaa accgcttaaa cttcttaata ttccattgaa
     8881 tgccggtatg agtatcggcg ttatgcttat cagtctcgaa cttaccatca tgctcatcat
     8941 tctgatgttt gttcctggtc aatatgcggg tgccagcttt atcggttttg ctattggtga
     9001 atcgctgggt gcttcggtac tgcgtattgc tggtggtatc tttacaaaaa ttgcggatat
     9061 cggctccgat ttgatgaagg tgattttcaa aatcggcgaa gacgatcccc gtaatcccgg
     9121 taccattgcc gactgcgtgg gtgataatgc cggtgatagt gtaggcccaa ctgcagatgg
     9181 ctttgaaact tatggtgtta ctggtgttgc tcttgtctct tttattttac ttgctgttgg
     9241 agggcctctt ttcaacatgg atgccgctgc cactcaggac ttgcagatta aactgctgac
     9301 ctggattttt gttatgcgag tacttatgat tattacctct gttgtttcat tctggattaa
     9361 tggttttatc agccgcgcac gctatcacaa tgccgatgat cttgattttg aaaaaccgct
     9421 cacatcgctg gtttggatta cttcaatcat ttcaattctg gttacttttg ctgttagtta
     9481 tgtcattatt ggcgatctgc cacacgatat gtggattacc cttagtatta tcatttctgc
     9541 aggtactttg ggagccgcgc tgattcccga gtttacaaag atttttacca gtccgaaatc
     9601 ggcacacgta caggaagtcg ttgagtcttc gcgtcgtggt ggagcatcgc tcaatatcct
     9661 ttctggttta gtggctggaa atttcagtgc cttctggcag ggcctcgtat ttgttgttct
     9721 tatggccatt gcttattttg ccagtctata tggccttggt gaagttatgg tctatccgtc
     9781 catttttgct tttggtttgg ttgcgttcgg tatgcttggt atgggtcctg taaccattgc
     9841 ggttgatagc tatggacctg ttaccgataa tgcacagtca atctatgagc tttcattgat
     9901 tgaagaagtc ccaaatgtta aagaagaaat tgaaaaagat tttggtttca cacctgattt
     9961 tgaaaagtca aagcattatc ttgaattgaa tgatggggct ggcaatactt ttaaagcaac
    10021 ggccaagccg gttttaattg gtactgccgt tgtaggtgcc accactatga tcttctcgct
    10081 tattttggtt attcaggagt ctctgggtgt aaatcccgaa cagattctga atattctgaa
    10141 tccatacact attctgggat ttattctggg cggtgctgtt gttttctggt tcagcggtgc
    10201 ttctattcag gccgtaacta ccggtgccgg acgtgctgtt gagttcatta aaaagaacat
    10261 taatcttgat ccgaatgcac ctaagaaagc tgatactgaa aaatcgaagc aggtggttgc
    10321 aatctgtacc aattacgcgc agaaaggtat gttcaatatc tttattgtta tcttctcttt
    10381 tgcattgggt attgctttcc tctcctcgcc tatagatggc gctgttgatc ccaatgcacc
    10441 ggtggctatg tttgttagtt atcttatctc aattgctttg tttggattgt ttcaggcagt
    10501 atttatggcc aatgccggtg gggcctggga caatgccaag aaggtaatcg aagtggatct
    10561 gaaagaaaaa ggaactgatt tgcatgcggc ggctgttgtg ggcgacactg tgggtgatcc
    10621 gttcaaggat acttcttctg ttgcactgaa tccaattatt aagttcacca ctttgttcgg
    10681 tttgttggcc atggaaatag ccatcagtga ggcattccgt ggtgtatcga aatatgtggg
    10741 agcgattttt attatcattg cccttatctt tgtgtatcgc agctttttct cgatgcgcgt
    10801 tgataaataa gaacagctct gatattaata aaaaaaggcc ggatttgtcc ggcctttttt
    10861 tatggttttg gataattcat attcaaaaat cggatatttt aaattaaaca cctccatcgc
    10921 gtggatttgt aatcatgagt gttcgaaacc tttcaaagag catatattat tgagcttaca
    10981 tggtt
//
LOCUS       DAOPKE010000090        10877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6335_length_10877_cov_11.749492, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000090 DAOPKE010000000
VERSION     DAOPKE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_10877_cov_11.749492"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..429)
                     /locus_tag="PKY63_10280"
     CDS             complement(100..429)
                     /locus_tag="PKY63_10280"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF01043.1"
                     /translation="MNCKVFSTLRFEKELKRLVKKFPSLKSEFAELIHKLQQTPDTGT
                     SLGNNCYKIRIKIASKGKGKSGGGRVITHFYKKDNSVFLLTIYDKSEKESVTQSEVKE
                     IISGLEF"
     gene            complement(419..628)
                     /locus_tag="PKY63_10285"
     CDS             complement(419..628)
                     /locus_tag="PKY63_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01044.1"
                     /translation="MKVILEIPDKQFEFAMKVLRSLSFVKKARPMSADAAKLWEDLKE
                     SADEVKMHKEGKLKLRTASDLLNEL"
     gene            627..1115
                     /locus_tag="PKY63_10290"
     CDS             627..1115
                     /locus_tag="PKY63_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01045.1"
                     /translation="MQKIIFAFMMQIYKNTTKITHCRFHEDLHRPDQLSPFPNAWMLN
                     EEVTPQDTPQVEQLLLFITAEHSRAEIHASFGLADKRNFVDKYLSPALHAGLIEMTIP
                     GKPKHRDQKYRLTESGILLKAQLEQKKRWFSAKKHTKWDTTFRSHTTQTRQRPKFNIL
                     HA"
     gene            1433..1705
                     /locus_tag="PKY63_10295"
     CDS             1433..1705
                     /locus_tag="PKY63_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPF01046.1"
                     /translation="MKKGIHPESYRFVVFKDTSNDYSFMTRSTVATKDTITWEDGKEY
                     PLVKLEISHTSHPFFTGKMKLVDTAGRVDKFKSRYQKHYEKKGENK"
     gene            1769..2980
                     /locus_tag="PKY63_10300"
     CDS             1769..2980
                     /locus_tag="PKY63_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HPF01047.1"
                     /translation="MNIILFDSFTRMHLLPLTFTRPVAELRVGITTISEKWQRWFPDS
                     VSCLAPDYIKPVFPVNVAADNILIDGSVLPDQQIVSAIQQLKENSVLTDTDNNILAAR
                     LPELQVQSLVSFPEYLDMRVLKPQSIETVKAFKAITHPWNLFTMAGELLTDDFTHLTK
                     GRKSAPLPASNRCVNPENIFIEDGATVEFSILNTSSGPIYIAKDAEIMENSVIRGPFA
                     LGEHSVVKIGAKIYGPTIVGPHCKVGGEVNNSVFLGYSNKAHDGFLGNSVIGEWCNLG
                     ADTNNSNLKNTYEEVKLWSYVKKGFVTTGLQFCGLIMGDHSKCSINTMFNTGTVTGVS
                     CNLYGDGFHRNFIPSFSWGSTAKLTDFQFEKAMLVVERVMARRGLQLSDSERDMLHHI
                     FSRTEEWHNKQ"
     gene            3058..5481
                     /gene="lon"
                     /locus_tag="PKY63_10305"
     CDS             3058..5481
                     /gene="lon"
                     /locus_tag="PKY63_10305"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPF01048.1"
                     /translation="MKKNKKDIFRDILFSDALTSDNEFIPLMSMEDEKTIQQEDIPDE
                     LPILPLRNTVLFPGVIIPITVGREKSIQLVRSVYKAKRIIGVVAQKDAQVEDPEPIDM
                     YDIGTVAYIIKLLQMPDDTVTIIIQGRSRFQINHFVKSDPYFIAKVQSYGRPQRLTGY
                     SKLFPTLMSTVKETAMQILKEGSEVPAEARIAIENIESPWLLMHMIASNLKIQHTEKQ
                     KLLEMTDLEQLGNDLLRHLTNELKMLEIKEQIHEKVKTDIDKQQRDYFLNMQLKTIQE
                     ELGGAPHEQEIKDLQEKAAKKKWSDNVAEVFDKEMSKLKRMNPSAAEFSIQLNYLEFL
                     VELPWNEFTNDNMNLDHARVILNEDHYGLEKVKERIIEYLAVLQLKGDMKSPILCLLG
                     PPGVGKTSLGRSIARALERKYIRMSLGGLRDESELRGHRKTYIGAMPGRIIQSLKKVK
                     SSNPVFVLDEIDKVSGNTMQGDPQAALLEVLDPEQNNTFYDNYLEVDYDLSKILFIAT
                     ANNLSTIHPALRDRMEIIDLSGYTQEEKLEIAKRHLIPKQLNENGMTKSKLKFQDRVI
                     NSIISDYTRESGVRALEKTIAKVIRHYATDYAMGKKIEPLKCEHLEKILGIPTFQRNK
                     AQLKPAIGLVTGLAWTVFGGEILYVEATLSKGKGALTMTGNLGDVMKESATLAFEYLK
                     SHAKELKIDAEKLDNYNVHIHVPEGATPKDGPSAGITIFTALTSVLLNKPVKNDIAMT
                     GEITLRGKVLPVGGIKEKILAARRSGIKTIVISEENRKDLGEIEDRYLEDISFHFIKE
                     MKEVLPIVL"
     gene            complement(5965..6378)
                     /locus_tag="PKY63_10310"
     CDS             complement(5965..6378)
                     /locus_tag="PKY63_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01049.1"
                     /translation="MKYICIPKADINEGFRSASVSRSAIVCFPDVIFIIPFRVSNIID
                     SSEERFNGAEIYREAAALLFSGGFAEAYDYLATAMPEEQTYYVKMLDKFSIKVGWWFF
                     GGISLRKKGGMLKTVSVTSRAKRQEMFEMYRYYVQ"
     gene            complement(6384..9500)
                     /locus_tag="PKY63_10315"
     CDS             complement(6384..9500)
                     /locus_tag="PKY63_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF01050.1"
                     /translation="MAIKKIFFSILFCSLLGSSAFSMNVKSLELTYENVSQLTYRAHV
                     VFYLYDPSPVDSIVEIFWGDSTYSFLTPSSVTDLPGELKKVVYTGTHTYSGPATFTMF
                     VSYKVRSLNVINVPMSLITDIYVEAELVVSPFLSGQKSPVFLDKPRMNVCRASDNVMD
                     YSAYDADGDELVYSLIPCRGEFGDPIAGYSYPASSAQFFIDSQSGWLTWDSPLQKGFF
                     NYAVKVSKYRNGIFLGSIMRDVLVNVIDCQPEMPVLTIPADTCIIAGQSFNQMVTATY
                     SGVDTISLTASGEPLLLVNNPATFPQPVRNQYTVSSDFAWNVFCDHVRPEPYTMIFRA
                     ELEDSLSNCDCTYDFNNWQLTPFYSNVSVMFNNPCGSGWDGSTYLWFGSDTTAPRFLA
                     TPSMDVSAGNYMIVFDMRFSDHTGNPGTDCEGPDEPDEGIYLQYSVNGLGGPWETMAY
                     WDPSLSPGEGGHVENLIGWNNYSVVAPDASLSANTRFRWVQFEATGRYYDHWGLDNIL
                     VYKISDKLTAQDQMQLSVIAPAPENLIVSAIGDHAFLSWNKEFCTNADGYKIYRKSGA
                     SGYVASGCETGVPAWTGYSLIETTTDINDTTYTDDNAGMGLPHGNEYCYIVTAWFANG
                     AESQASNEACIVLSDDSPVITHVSVTATSVSAGSMFVDWSKPDDFDTLLYSGPYRYDI
                     YRASDFSGSSYTFLTSNDGLDDTTFVDAGLNTLDGPWHYRIDLMYASGTAVYAPFTSS
                     YPASSVFLSLTPLDKKLLLQWNFNVPWNNDSTVIYRYNNLTATFDSIGISYSDTYLDT
                     GLVNGVTYCYKVETIGSYSLPGFVAPIRNFSQEQCEQPMDVEPPCPVELSVSVNCDEV
                     ANYLVWTNPQTTCGDGDVGSYEIYFSSMTDGEFVQIAAVSGAETTTYIHNGITSVAGC
                     YAVVAVDTSGNSSGFSNIVCVDIDECDLYSLPNVFTPNGDGFNDLFVPFPYDFVEKID
                     LKIFDRWGLIMFTTQDPDINWDGKNQSTQADCSEGVYFYVCDVYELRLAGIRKRTLTG
                     TVHLYRNY"
     gene            9946..>10877
                     /locus_tag="PKY63_10320"
     CDS             9946..>10877
                     /locus_tag="PKY63_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01051.1"
                     /translation="MKIKNILLIATTLTGITINNASAQCFAEIETPAQDTTICVGESV
                     YLSAFATCTFLMSNNFNNGTAGTGWVATTGVSFTNPCNPSSDGTIYMWMGDAVPLPRT
                     LTTVPFDVTGSCEISFDMKYATQSQSSPCEGIDEYNEGVSLQYSINSGATWVDIAYFQ
                     PDGVILTSNPGTSGTCISSPYVTPFTTWANYTFPIPPAAQSSATMFRWIQIDYSSLTN
                     DHWGLDNVEIVCPQSQNIFWSNGVSNALSQTVTPTTTEYIVAIFDSLGNIATDTIMVN
                     VIPTPSASFDTESPVCINQPSTINFTGSNNPGVT"
BASE COUNT         3353 a         2499 c         2202 g         2823 t
ORIGIN      
        1 cccaacgcca gaatgtcgcc tccgacgacg attccgggat gagggaaatt aagcgcagtg
       61 ccggtttgca ttcattgtcc agcgccaatt aaatctccat tagaattcaa gacctgaaat
      121 tatttctttg acttcgcttt gagttactga ttctttctcg ctcttgtcgt aaattgtgag
      181 taagaaaaca gaattgtctt ttttgtaaaa gtgagttata actcttccgc cacctgattt
      241 tccttttcct ttggaagcta ttttaattcg gattttataa caattattac caagcgatgt
      301 tcctgtatcc ggcgtctgtt gcaatttatg aattaattct gcaaattctg atttcagcga
      361 agggaatttt ttcaccaatc gctttaattc tttttcaaat cggagtgtgg aaaaaacctt
      421 acaattcatt gagtaaatcg cttgcagtac ggagtttgag tttaccttcc ttgtgcatct
      481 ttacttcatc agccgattct ttcagatctt cccacaattt tgctgcatcg gccgacattg
      541 gccgcgcttt tttcacaaaa gataggcttc gcaacacttt cattgcaaat tcaaattgtt
      601 tgtcgggtat ttcaagaatg actttcatgc aaaaaataat atttgctttt atgatgcaaa
      661 tatacaaaaa tactacgaaa atcacacatt gcagatttca tgaggatttg cacagaccgg
      721 atcaattgtc acccttccct aatgcctgga tgctgaatga ggaggttacc ccccaagata
      781 ccccccaagt tgagcagctt ttgcttttca ttactgcaga gcattccaga gcagaaattc
      841 atgcttcatt tggtctggcg gataaacgaa atttcgttga caaatacctg tcaccagctc
      901 tgcatgcagg attaattgaa atgactatcc caggcaagcc gaaacatcgc gaccaaaaat
      961 acagactcac tgaaagtggt attcttttga aagcgcagtt agagcagaaa aaaaggtggt
     1021 tttcggcaaa aaagcacaca aaatgggaca ccacattccg ctcccacact acccaaaccc
     1081 gccagcgacc caaatttaac attttgcacg cttagaaata attttctatc tttactcaac
     1141 gttaatttcc catgcccgaa atatctttcc atacaacata ttcaaataat tgtcatttgt
     1201 ttgcgtgcgc agagaatggc ttccagagcg gcctgcagag gcgcaggtgt caccgtttcg
     1261 aattcaatgg tattgaacca taatcatagc gagaacgcag ctttgccatg ctccataccc
     1321 tgtgcattgt gcgttgagtc gcatgctaat gcatcaaatt gaaaaatatt ttctgacaat
     1381 tttgtttatt cagaaatatt ttctaatttt gcactcccaa ataaaaaagg aaatgaaaaa
     1441 aggtattcat cccgagagct acagattcgt tgtattcaaa gatacatcaa acgattattc
     1501 tttcatgacc cgttcaaccg tggccacaaa agacactatt acatgggaag acggcaagga
     1561 atatccgctc gtgaaacttg aaatctcaca cacctcgcat ccgttcttta ccggaaaaat
     1621 gaaactggtt gataccgccg gtcgtgttga taaattcaaa agccgctatc agaaacatta
     1681 cgaaaagaaa ggcgaaaaca aataaattgt ttgcttcaaa atactgtaaa agccctcgaa
     1741 acagggcttt ttttattatt tttacgccat gaatatcata ctgttcgata gcttcacgcg
     1801 tatgcatttg ctgccgctaa cgttcacccg accggtggca gaactcagag ttggcataac
     1861 caccatttcg gaaaaatggc agcgctggtt ccctgattcc gtttcgtgtc tggcacccga
     1921 ctacatcaaa ccggtttttc cggtgaatgt ggcagctgat aacatcctga ttgatggttc
     1981 tgttcttccc gatcaacaga ttgtaagcgc cattcagcaa ctgaaggaaa acagcgtcct
     2041 gaccgatacc gataacaata ttctggcggc acgcctgccc gaattgcagg tacaatcact
     2101 ggtttcattt ccggaatacc ttgatatgcg tgtacttaag ccccagtcaa tcgaaactgt
     2161 taaagctttc aaagccataa cgcatccatg gaacttattt accatggcag gcgaactgct
     2221 gaccgatgat tttacccatc tgacaaaagg ccgcaaaagc gctcccctgc ccgcttcgaa
     2281 ccgctgcgtg aatccggaaa atatatttat tgaagacggt gcgactgttg aattttccat
     2341 tctgaatacc tcctcaggcc cgatttatat tgcaaaagat gctgaaataa tggaaaacag
     2401 tgtcattcgc ggaccatttg ctttgggcga gcattcggtc gttaaaatag gcgccaaaat
     2461 atacggacca accattgtag gaccgcactg caaagtcggc ggtgaagtca acaactccgt
     2521 ttttctggga tattcaaaca aagcccacga cggatttctc ggcaattcag tcattggcga
     2581 atggtgcaac cttggcgccg acaccaataa cagcaacctg aaaaacacct acgaagaagt
     2641 caagctctgg agctatgtaa agaaaggttt tgttacaacc ggtctgcagt tttgcggtct
     2701 catcatgggc gatcacagca aatgcagtat caacactatg ttcaataccg gtacggttac
     2761 tggcgtttca tgtaatctgt atggcgatgg ttttcaccga aatttcattc cctcgttttc
     2821 gtggggcagt accgccaaac tgacagattt tcagtttgaa aaagccatgc tggttgttga
     2881 gcgcgtaatg gcacgcagag gccttcaact atctgattca gaaagagata tgttgcatca
     2941 tattttttcc agaaccgaag aatggcacaa taagcaataa ttacattctt ttggcattta
     3001 tattggcaag ggggtacact ccgcataaat cacgcggaac acagacattt tgacagcatg
     3061 aaaaaaaaca aaaaagatat tttcagagat attctcttca gcgacgcatt aacttccgat
     3121 aatgaattca ttccgctcat gtcgatggaa gatgaaaaaa ccatacagca ggaagacatc
     3181 ccagacgagt tgcccattct gcctttgcgc aatacagtac tttttcccgg agtaattatc
     3241 cccattactg tcggtcgcga aaaatcaatt caattggtcc gtagtgtcta taaggccaag
     3301 cgaattatcg gcgttgtagc tcaaaaagat gcacaggttg aggatccgga acccatagat
     3361 atgtacgaca ttggaactgt tgcctacatc atcaagctat tacagatgcc cgatgacacg
     3421 gtaacgatta tcatacaggg ccgcagccgc tttcagatca atcattttgt aaaatcggat
     3481 ccctatttta ttgccaaagt gcagtcctat ggccgtccac aacgccttac gggctattcg
     3541 aaactcttcc ccaccctcat gtcaaccgta aaagaaacgg ccatgcagat tctcaaggaa
     3601 ggttccgaag tcccggccga agcgcgcatt gccatcgaaa acatcgaaag cccctggttg
     3661 ttgatgcata tgattgcttc gaatctgaaa attcagcata ccgaaaaaca gaaactgctc
     3721 gaaatgaccg atctggagca gcttggcaat gatttactgc gtcatctgac caatgaactg
     3781 aaaatgcttg aaatcaaaga gcaaattcac gaaaaagtaa aaacagatat cgacaaacag
     3841 cagcgcgatt attttctgaa catgcagtta aaaaccattc aggaagaact gggtggtgca
     3901 ccgcatgaac aggaaataaa agatttacag gaaaaagcag ccaaaaagaa atggtcggac
     3961 aatgtcgccg aagtatttga caaggaaatg tcgaaactga agcgaatgaa cccttcggca
     4021 gcagagtttt ctattcagct caactatctt gagtttttgg ttgaattgcc ctggaacgaa
     4081 tttaccaacg acaacatgaa tcttgatcac gcccgtgtta ttctcaatga agaccactac
     4141 gggctggaga aagtgaagga aagaattatt gaatacctgg cagtgctgca actgaaaggc
     4201 gatatgaaat ctccgattct ttgtctgctt ggccctccgg gtgttggcaa aacctcactc
     4261 ggacgaagca ttgcacgagc gctggagcgc aaatatatca gaatgtcgct gggcggtctg
     4321 cgcgacgaat cggagctgcg cggacatcgt aaaacctata taggcgctat gcccggacga
     4381 atcattcaga gtctgaaaaa agtaaaatca tcgaatcccg tatttgtact tgatgaaatt
     4441 gataaagttt cgggaaatac catgcagggc gacccgcagg cagcactgct cgaagtgctc
     4501 gatccggagc aaaacaatac tttttacgat aactatctcg aagttgatta cgatttgtcg
     4561 aaaatccttt ttattgcaac ggccaacaac ctttccacca ttcatcccgc gctgcgcgac
     4621 cgtatggaaa tcattgacct gagcggttac acacaggaag aaaaactgga aattgccaaa
     4681 cgacatttga ttccgaaaca actcaacgaa aacggaatga cgaaatctaa actgaaattt
     4741 caggatagag tcatcaattc cattatatca gattataccc gcgaatccgg agttcgtgca
     4801 ctcgaaaaaa ccattgctaa agtgatacgg cattatgcta ccgactatgc catgggtaaa
     4861 aaaattgagc cgctgaaatg cgaacacctt gaaaaaattc ttggaattcc aacattccaa
     4921 cgcaacaaag cacagctaaa acccgccata ggtcttgtta caggattggc atggactgtt
     4981 ttcggtggtg aaatcctgta tgtcgaagcc actctgagta aagggaaagg cgcacttacc
     5041 atgaccggaa atctgggcga tgtaatgaaa gaatcggcca cacttgcctt tgagtacctg
     5101 aaatcgcatg caaaagaact gaaaatcgat gccgaaaagc tcgataatta taatgtgcat
     5161 atccacgttc ccgaaggcgc cacaccgaaa gacggacctt cggcgggaat taccattttc
     5221 acagcactca ccagcgtgct tttaaacaaa ccggtgaaaa acgacattgc catgaccggc
     5281 gaaattaccc tgcgcggaaa agtacttcct gtcggtggaa ttaaggaaaa aattctggct
     5341 gcacgccgca gcggaatcaa gaccattgtt atttctgaag aaaaccgcaa agacctcggc
     5401 gaaatcgaag accgctacct cgaagacatc agcttccatt ttataaaaga aatgaaagaa
     5461 gtgctgccga ttgttttgta gaatcagctt ttagtaatca gcaatcagca attgtgccgg
     5521 ccgaagcgtc ggtgctctgt gaaggagcca aatatcagag cgggaatcag agcgagagcg
     5581 aaactatttc atacgcaatg ctccatgcac tttgcgttgt gcattgtgcg ttgtgcattg
     5641 caaccgcatg ccgcctgcct ttacatcgcc tttatacact ttcaactctt ttgtccacgg
     5701 attacactga ttacgcggac ccgcctgcag cgggcaggtt aaaactccct ctgctggtct
     5761 ttggatttca gcggataaaa tgtctctcaa cctgcgactg aagccatttc cacagataat
     5821 tgtacaaaat ttttcagtca atgattatta cgcagtaata attgttgaca acagtgtaat
     5881 caggatgggg ccactgaagg taaagtgttt ccgtgcaatc tgtgtaatcc gtggacctat
     5941 tctttctgcc acttgccgca tgcgctattg cacataatac cgatacattt cgaacatctc
     6001 ctgacgcttc gcccggctgg tcaccgatac agttttcagc attccgcctt ttttacgcaa
     6061 gctgataccc ccaaaaaacc accaaccgac tttgatactg aatttatcca gcatttttac
     6121 ataataggtt tgttcttcag gcatggctgt agcaagataa tcgtatgctt cggcaaaacc
     6181 gcctgaaaat aacaatgcag cggcttctct gtaaatttca gctccattga aacgctcttc
     6241 agacgagtct ataatattgc ttacccgaaa aggaataata aaaatgacat ccggaaaaca
     6301 aacaatggca gagcgactca ctgacgcaga gcgaaatcct tcattaatat cggcctttgg
     6361 aatgcagata tatttcattc tgtctaataa tttctgtaca aatgcaccgt cccggtcaat
     6421 gtgcgttttc tgattcccgc aagacgcaat tcatacacat cacacacata gaaataaacc
     6481 ccttcgctgc aatcggcctg cgttgactga ttttttccat cccagttaat atcaggatcc
     6541 tgagttgtaa acataataag cccccaacga tcaaatatct ttaaatcgat tttttctaca
     6601 aaatcatacg gaaaaggaac aaacaaatca ttgaatccat ctccattcgg agtgaataca
     6661 ttgggcaaag aatataaatc gcattcatca atatcaacac aaacaatatt cgaaaaacca
     6721 ctgctgttgc cggatgtatc aacagccacc acagcataac aacccgctac ggaagtgata
     6781 ccgttgtgga tataagttgt ggtttcggct ccggaaactg ctgcaatctg aacaaactca
     6841 ccatcagtca tcgatgaaaa ataaatttca taactgccaa catcgccatc gccacaagta
     6901 gtttgcggat ttgtccacac aagataattg gctacttcgt cgcaattaac cgatacactc
     6961 agctcaaccg gacaaggtgg ctcaacatcc atgggttgtt cgcattgttc ctgcgaaaaa
     7021 ttgcggatag gcgcaacaaa tcccggcagt gaataggatc cgatggtttc aactttgtaa
     7081 caataggtta ctccattcac cagaccagta tcgagataag tatccgaata tgaaataccg
     7141 atggaatcga acgttgcagt cagattattg tatcggtaaa tcactgtgct gtcattgttc
     7201 cagggcacat taaaattcca ttgcagcagc aactttttat ccaacggggt cagcgaaaga
     7261 aaaaccgaag aagccggata ggaagaagtg aaaggagcat ataccgctgt gcccgaagca
     7321 tacatcaaat caatgcgata atgccacgga ccgtccaggg tgttcaaacc agcatcaaca
     7381 aatgtggtat catcgagtcc gtcattggaa gtcagaaaag tgtaggatga tcctgaaaaa
     7441 tctgaggcac ggtaaatatc gtagcggtaa ggtcctgaat ataaaagtgt gtcaaaatca
     7501 tcgggtttcg accaatcaac aaacatactt ccggccgaaa ccgaagtggc agtgacactt
     7561 acatgagtaa ttaccggtga atcatcggac aatacaatgc aagcttcgtt cgaggcctga
     7621 ctttcggcac cgttggcaaa ccatgctgta acaatgtagc agtattcatt gccatgaggc
     7681 aaacccatcc ctgcattgtc atcggtatat gttgtatcgt tgatatcggt agtggtttca
     7741 atcagactat atccggtcca agccggaacg cccgtttcgc aacccgaagc cacataacca
     7801 ctggcgccgg atttacggta tattttatag ccatctgcat tggtgcagaa ttctttgttc
     7861 cagctcagga atgcatgatc gcctatggca gaaacaatca gattttcagg cgcaggtgca
     7921 atcaccgata attgcatttg gtcctgcgct gttaatttat ccgaaatttt ataaaccaaa
     7981 atattgtcaa gaccccaatg atcgtaataa cgaccggtag cttcgaactg aacccagcga
     8041 aaacgtgtat tggccgacag cgatgcatcg ggtgccacca ccgagtaatt gttccatcca
     8101 atcaggtttt cgacatggcc accttcaccg ggcgaaagac tcggatccca gtaagccatg
     8161 gtttcccatg gaccaccaag accattaacg ctatactgaa gataaatacc ttcatcgggc
     8221 tcatcaggcc cttcacaatc tgtacccgga ttgccagtat gatcgctgaa acgcatatca
     8281 aaaacaatca tataattacc ggcactgaca tccatcgacg gcgtagccag aaaacgcggg
     8341 gccgtagtgt ctgaaccaaa ccacagataa gtcgaaccat cccatccact tccgcaagga
     8401 ttgttgaaca taacggaaac attggaataa aacggtgtca gttgccagtt gttgaaatca
     8461 taggtgcaat cgcaattgct cagactgtct tcgagttcag ctctgaaaat catggtgtac
     8521 ggctcgggtc gaacatgatc acagaacaca ttccatgcaa aatccgatga aacagtatat
     8581 tgattgcgaa ccggttgcgg aaaagtggcc ggattattca ccaacaaaag cggctcgccc
     8641 gaagctgtaa gcgaaatagt gtcaacacca ctataagtgg cagtaaccat ctgattgaat
     8701 gactgaccgg caataatgca ggtatctgcc gggatcgtca acacaggcat ttcgggctga
     8761 cagtcaatga catttaccaa cacatcccgc atgattgatc caagaaaaat tccattgcga
     8821 tatttggaaa ccttaacagc ataattgaaa aaacccttct gcagaggaga atcccacgta
     8881 agccagccac tttgagaatc aatgaaaaac tgtgctgacg aagccggata agaatagccg
     8941 gcaatgggat cgccaaattc gccacggcat ggaatcaggg aataaacaag ttcatctcca
     9001 tcagcatcat aggccgaata atccatcaca ttatccgaag ccctgcatac attcattcgg
     9061 ggcttatcga gaaaaaccgg tgatttttgc cctgaaagaa aagggcttac aacaagctcc
     9121 gcctcgacat atatatcggt aataagcgac atgggaacat tgatgacatt gagactgcgc
     9181 accttgtatg aaacaaacat ggtaaatgta gccggtccgg aataggtatg ggtgccggtg
     9241 taaaccactt tcttcagttc gccgggcaaa tcagttaccg atgagggcgt aagaaaagaa
     9301 taagtgctgt cgccccaaaa aatctcaaca atagaatcaa cggggctggg atcgtagaga
     9361 taaaaaacaa catgtgcccg gtaagtcagc tgagaaacat tttcatatgt caactcaagc
     9421 gatttgacat tcatcgaaaa cgcagaactg cctagcagag aacaaaacaa tatggagaaa
     9481 aatatttttt tgattgccat cgcttaaaaa cacagattgc aaacgtaaaa gtacaaaaaa
     9541 aattatgcat ttcattatat aaatgaacct gcatgcaaaa tatttaagaa acaaatatgg
     9601 cttttagaaa aaagttgcaa ccattttaaa taaagaacac aaaagctcaa ataaaaaaaa
     9661 ttgcgccgaa tatttaactt tttacgaatt ttatagtcaa accactgttt ttttgatgat
     9721 tacagcaaga tgttatattc tattaagtga taaacatcag ttgtttttgg aaaaagaact
     9781 gaaatttctt aaatttgcat atcgaatgcg tttgaattaa acagcgtgct attgggggat
     9841 aattttcgcg gtcttatcag acatatttag cattcgtttt aatcaaaagc aaatttggaa
     9901 caaggaactg cttttctgaa atcaagacag acttaaaaag gaccaatgaa aataaaaaat
     9961 attctgctga ttgcaacaac tctgacgggc attactatta ataatgcttc ggcgcaatgt
    10021 tttgcagaaa ttgaaactcc tgcacaggac acaacaatct gcgttggaga atcggtgtat
    10081 ttaagtgcat ttgccacctg cacattcctg atgagcaaca atttcaacaa tggaactgcc
    10141 ggcactggct gggttgcaac tacgggcgtg agttttacaa acccatgtaa cccatcatca
    10201 gatggaacca tctatatgtg gatgggcgat gccgtgccgc tgccgcgcac actaaccact
    10261 gtacctttcg atgttacagg atcctgtgaa atttcctttg atatgaaata tgcaactcaa
    10321 tcgcagtcat cgccctgcga aggaattgat gaatacaacg aaggtgtttc acttcaatat
    10381 tccatcaaca gcggagctac atgggtggat attgcatatt ttcaaccgga tggcgttata
    10441 ctcacttcca atccaggcac ttccggaacc tgcatttctt caccttatgt tactccattc
    10501 actacctggg ctaattacac ttttcccatt ccgccggctg cccaatcatc tgctacaatg
    10561 tttcgctgga ttcaaatcga ttattccagt cttacaaatg atcattgggg tcttgataat
    10621 gtagaaatcg tttgccctca atctcagaat attttctggt caaatggtgt ttccaatgct
    10681 ttatcgcaaa ctgtaacacc caccaccacc gaatacattg ttgccatttt cgactctttg
    10741 ggaaatattg caaccgatac cattatggtg aatgttatcc caactccttc ggccagcttt
    10801 gataccgaat cgccggtttg tatcaatcaa ccgtcaacca tcaatttcac gggttcaaac
    10861 aatcccggtg ttactta
//
LOCUS       DAOPKE010000091        10874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6339_length_10874_cov_12.359922, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000091 DAOPKE010000000
VERSION     DAOPKE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6339_length_10874_cov_12.359922"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PKY63_10325"
     CDS             complement(<1..1141)
                     /locus_tag="PKY63_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF01052.1"
                     /translation="MKTEEKIKQVLTKFGIGEINDGVSTGTVWIKSKGDTIISVSPID
                     NREIAKVKNATADDYEFVIKKAQEAFKVWREVPAPQRGEIVRQIGLALREAKEDLGFL
                     VSLEMGKIHQEGMGEVQEMIDICDFAVGQSRLLNGFTMHSERREHRMYDQYHPLGVVG
                     LITSFNFPVAVWAWNSMLAAVCGDVVIWKPSSKTPLCAIATQNIISDVLKRNNIPEGV
                     FNLVVAKSSVLGDNFVADKRIPLFSITGSTRVGKRAGAIIGERLGKSILELGGNNAVI
                     ISDSADLNMAIASTVFGAVGTCGQRCTSTRRIIVHKKVYNEVKYNLLSSYEQIKEKIG
                     NPLDPNVLIGPLVDDKAVDAFLHAIEEVKKEGGKVLFGGTKVEGKK"
     gene            complement(1169..2794)
                     /gene="pruA"
                     /locus_tag="PKY63_10330"
     CDS             complement(1169..2794)
                     /gene="pruA"
                     /locus_tag="PKY63_10330"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPF01053.1"
                     /translation="MNNAIFDFENPHNEKILNYAPGSNERILLTNELRELESSRIEIP
                     LIIGGKEITTGKFGKIVMPHDHEHVLATYHMATEKEAKMAVDAAMKAHEVWAETDWTV
                     RAAILLKAAELLSTKYRSLINASTMLGQSKNAYQAEIDSACETIDFLRYNAYFASKIY
                     SEQPKSGFNQLNKMEYRALEGFVFAVSPFNFTSIASNLNMSPVLMGNTTIWKPATTAI
                     FSNYYLMKVFMEAGLPDGVINFLPGSGSLIGKTVFGSKDLGGIHFTGSNNTFNSLWRS
                     VSENLNMYKSYPRIVGETGGKDFVFVHPSADQLEVAVGIIRGAFEYQGQKCSAASRAY
                     IPKSLWQGIKDLLIQMTSEIGLGDVRDFKNFMNAVIDEASFDNIMGYINFAKTSGDAK
                     IIAGGSGDKTKGFFIQPTIIETTNPKFKTMEEEIFGPVITIYVYEDKDYDSALTLCES
                     TSPYALTGAVFSRDRVAAAQASHRLKNAAGNFYINDKPTGAMVGMQPFGGARASGTND
                     KAGGNLNLMRWTSPRTIKETFAPATDFRYHFMM"
     gene            3020..4921
                     /gene="htpG"
                     /locus_tag="PKY63_10335"
     CDS             3020..4921
                     /gene="htpG"
                     /locus_tag="PKY63_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPF01054.1"
                     /translation="MQKGSINVQTGNIFPIIKKFLYSEHEIFLRELVSNAIDATTKLK
                     KLVSLGKADVELGDLTIQVALDAKKKTLKITDRGIGMTEEEVQKYINEIAFSGAEDFV
                     KKYAGKDDKEISGIIGHFGLGFYSSFMVSDKVTIDTLSYQDGAKAVKWECDGSPEYTM
                     SESKRKDRGTEITLHLSEDSIEFNEEYRILDMLKKFCRFLPYPIQFGMEKTSEPIEGE
                     KDKDGNQKYEDVEKPRIINNTDPLWKKTPTELKDEDYKNFYRELYPYSYDEPLFNIHI
                     NVDYPFNLTGILYFPKLKKNLEVQRNKIQLYSNQVFVTDNVENIVPDFLTLLHGVIDS
                     PDIPLNVSRSYLQGDPNVKKISSHITKKVADKLEELYKNSREDFEKKWDDIRVFIRYG
                     MISTESFAERAEKFALLKDTEGNYCTLEEYKEKIKEAQTDKDDKLVILYTTDKEEQHT
                     YISAAQAKGYSVLEMDSVIDIHYIDFLERKNEKISFKRVDAELTDKLIDKNVETIVSK
                     LNEDESKKVEELFKGALPADVQVFVKVEAAAEDSTPVSMMQPEFMRRMSDMSQLGGMD
                     YMAGMGRSYTLLVNSNHPVVTGLVDSSDEEKNKNVVSQLIDLALLSQGLLKGEKLSRF
                     INRSIDIIK"
     gene            4958..5623
                     /locus_tag="PKY63_10340"
     CDS             4958..5623
                     /locus_tag="PKY63_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPF01055.1"
                     /translation="MENTTQTLPGGSQPNRSNPKPKTGCLGSTMIIILLLIILLPIGI
                     WMWRAYNRMVTKDELVTKQWANVENVYQKRMDLVQNLVNTVKGMADFEKSTLTDVIEA
                     RSKASSININADNLDEETFAKFQQAQDEFSGALSRLMVVMEQYPQLQAVEGFKNLMND
                     LKGIENEILAQRNAYNEVAMDYNQYIRTFPRNLFASLFGFDKKGYFKSSAGAENAPTV
                     SFE"
     gene            5863..6087
                     /locus_tag="PKY63_10345"
     CDS             5863..6087
                     /locus_tag="PKY63_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HPF01056.1"
                     /translation="MKQIRPLQKVWKFYADGFRNMPSWGRQAWAVVIFKGIVVFIIMK
                     FVFFPNHLKKNFDTDEQRSEHVLDQLTKTK"
     gene            6091..7659
                     /locus_tag="PKY63_10350"
     CDS             6091..7659
                     /locus_tag="PKY63_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPF01057.1"
                     /translation="MDQISTLVDWSRAQFALTAMYHWIFVPLTLGMTFMIAFMETLYV
                     RSGNEEWKRITKFWAKLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLAVE
                     GIMAFFMESTFIAIMFFGWNKVSKKFHLLSTWLVAIGANLSALWILVANGWMQFPVGT
                     VFNPETARNEMVDFWAVLFSPVAVNKFLHTISNGFVVSALFIIGVSAWFILKKREIVF
                     AKKSMLIAATFGLLSSIFLALTGDGSAYQVAQKQPMKLAAMEGLYKGQKEAGLIMVGM
                     ITPGKQPGDNQADYVFKLEIPKLLSFLGYRDAKAFVPGIDDLVYGNEEQGLISAKEKI
                     ERGTIAVETLRKFKQAKQSGDSTKAAELALLFDRKTTAGQDFYNNYFRYFGYGHIEKP
                     EDIVPNVPLVFYSFHIMVALGFFFIFFFAVMLFFLMKNSVEKRKLWLHLAIWTFPLGF
                     IASQAGWIVAEVGRQPWVIQDLMPVNTAVSQVSASAVQLTFVLFAIIFTSLLIAEISI
                     MTRAIKNGPTEGGK"
     gene            7661..8800
                     /gene="cydB"
                     /locus_tag="PKY63_10355"
     CDS             7661..8800
                     /gene="cydB"
                     /locus_tag="PKY63_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPF01058.1"
                     /translation="MFETFSYLALQEYWWFIISILGAFLVFLLFVQGGQSLLYTIGKE
                     EPQTSMLVNSLGRKWEFTFTTLVTFGGAFFASFPLFYSTSFGGGYWLWMLILFTFILQ
                     AVSYEFRSKQGNVFGKKTFEWFLFLNGFIGTVSLGVAVATMFTGGNFVHDDMNFTSWS
                     NNWHGLEAFANPINVALGLAVFFLSRVLAILYFNSTINDGIIRERNKKHLLINSVLFL
                     IFFLAFVIAIWLIDGYAYNPATGEIFTEPHKYFYNNIEHALTGILFLLGVLMVLLGLF
                     RGNTCYKSCSGKALYPVGIGTFLTVLSLFCNLGYNNTVFYPSLSSMQSGLTIEKASSS
                     QFTLTVMSYVSLLVPFVLAYIIYAWHALNKKKIDVDEMNDGSHKY"
     gene            9077..9934
                     /locus_tag="PKY63_10360"
     CDS             9077..9934
                     /locus_tag="PKY63_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPF01059.1"
                     /translation="MEKNIKPSLGYLALAISILMLLSPFLIFADESNLLIITGTIVLS
                     FLSIFGFIGLFVVNPNESMVLILFGKYMGTARENGFFWVNPFYVKKKISLRARNMESE
                     SLKVNDKMGNPIIISVVMVWRIRDTYKAAFEVDNYEIFVKTQTEAAVRKLAHTYPYDT
                     FDDAQEEISLRGGGDEINAELEREISERLAIAGVEVLESRLSNLSYAPEIAGAMLQRQ
                     QATAIIAARTRIVEGAVSMVDMALNQLSKKGIVELDAERKAAMVGNLMVVLTSERAAQ
                     PIVNAGTLY"
     gene            9947..10471
                     /locus_tag="PKY63_10365"
     CDS             9947..10471
                     /locus_tag="PKY63_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01060.1"
                     /translation="MTTFSETQHTQKPLFIAIPIVLWLTFMVFAVIELYNYLHFGIPL
                     GDKGEEIPLGGFIGIMILLSLVFAFFLFMVLRLQLKISISESGIYFEMPPFKKPATFK
                     PEEIESAFVRKYYPIRQYGGWGWRGIKDKAYNVSGRWGIQIVLKNKKRILLGIQNHED
                     AARALESIGFNKQL"
     gene            10474..10662
                     /locus_tag="PKY63_10370"
     CDS             10474..10662
                     /locus_tag="PKY63_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPF01061.1"
                     /translation="MGKKAFALRIDEEVMKALEKWAADEFRSVNGQIEWILHQAMAKA
                     GRAPKQKNSEHRTRKDEG"
BASE COUNT         3100 a         2438 c         2304 g         3032 t
ORIGIN      
        1 tttttttacc ttcaaccttg gttccgccaa acagaacctt tcctccttct ttttttactt
       61 cttcgatggc atgtaaaaaa gcatccaccg ctttatcatc caccaaggga ccaatcagaa
      121 cattcggatc caacggatta ccgatttttt cctttatctg ttcgtaggaa ctcagcaaat
      181 tatacttcac ttcgttgtac actttcttgt gaacaataat gcggcgcgta gatgtacagc
      241 gctggccaca ggttccgact gcaccaaaaa ccgtcgatgc gatggccata ttcaaatcgg
      301 ctgaatcgga aataatgaca gcattgtttc cccccaattc aagaatcgat tttccgagac
      361 gttctccaat aatggctccg gctcttttac ctacgcgggt tgaacctgtg attgaaaaca
      421 acggaatgcg tttgtcggca acaaaattat cgcccaatac tgaagatttg gcaacaacca
      481 aattgaaaac accttcagga atattattgc gttttaatac atccgaaata atattttgtg
      541 ttgcaatggc acaaagcggt gtttttgagc tgggtttcca aataacaaca tcgccgcaaa
      601 ctgctgccag catagaattc catgcccaaa cagccacagg gaaattaaaa gaagtaatta
      661 aaccaacaac acccaatgga tgatactgat cgtacatgcg atgctcgcgg cgctccgaat
      721 gcatggtgaa tccattcagc aatcgtgatt gtccaaccgc gaaatcacag atatcaatca
      781 tttcctgcac ttctcccatg ccttcctgat ggatttttcc catttcgaga gaaaccagaa
      841 aacccagatc ttctttggct tcgcgcaagg caagaccaat ctgacgaacg atttcgccac
      901 gttgtggagc cggcacttcg cgccagactt taaaagcttc ctgcgccttt tttataacaa
      961 attcataatc atctgccgta gcatttttca ctttggcaat ttcgcgatta tcgatcggcg
     1021 aaacagaaat aattgtatcg cctttgctct taatccacac agtacctgta gaaacgccat
     1081 cattgatttc gccgatgcca aattttgtga gtacctgttt gattttttct tcagttttca
     1141 ttgtatgata acagttgttt tagttgtttt acatcatgaa atgataacgg aaatcggttg
     1201 ccggagcaaa ggtttccttg atggtacgcg gacttgtcca tcgcatcaga ttaaggttcc
     1261 cgccagcttt gtcgttggtt cccgaagcgc gcgcgccgcc gaatggctgc atgcccacca
     1321 tggcgccggt cggtttatcg ttgatataga aattaccggc cgcatttttc aacctatgac
     1381 tggcctgggc agcagcaacc cgatcacgcg aaaaaacagc gcccgtcaat gcatatggag
     1441 atgtgctttc acaaagcgtt aaggccgaat cataatcttt gtcctcatat acatatatgg
     1501 taattaccgg tccgaaaatt tcttcttcca tggttttgaa cttcggattg gtggtttcga
     1561 taatggttgg ttgaataaaa aaccccttgg tcttgtcgcc tgacccacca gcaatgattt
     1621 tggcatcacc cgatgttttt gcaaaattaa tgtagcccat gatattatca aacgaggcct
     1681 catcgattac tgcattcatg aaattcttaa aatcacgcac atcaccgagc cctatttccg
     1741 aagtcatctg aatcagcaaa tccttaattc cctgccacag cgattttgga atatatgcac
     1801 gcgaagctgc agagcacttt tgtccctgat attcaaaagc gccacgaata attccaaccg
     1861 ccacttcaag ctgatcggcc gatggatgca caaaaacaaa gtcttttcca ccggtttcac
     1921 caacaatacg cggatacgat ttatacatat tcagattttc gctcacagaa cgccaaagtg
     1981 aattaaaggt attgttcgat ccggtaaaat gaattccccc aaggtcttta ctgccaaaaa
     2041 cagttttacc aatcaatgat ccactgccag gcaaaaagtt gattacccca tctggcaatc
     2101 ctgcttccat aaaaaccttc atcaggtaat aattagagaa aatggctgtg gtagccggtt
     2161 tccatatagt agtattgccc atcagcaccg gcgacatatt cagattcgac gcaatcgaag
     2221 tgaaattaaa cggcgaaaca gcaaacacaa atccctccag cgcccggtat tccatcttat
     2281 tgagctgatt aaaacccgat ttgggctgct cactgtaaat ttttgatgca aaataggcgt
     2341 tgtagcgcaa aaaatcaatt gtttcacagg cagaatcgat ctcggcctga taggcatttt
     2401 tgctttgccc gagcatggtt gatgcattaa tcagagaccg gtatttggta gaaagcaatt
     2461 cggctgcttt gagcaaaatg gctgcccgaa cagtccaatc ggtttcggcc cagacctcat
     2521 gagctttcat tgcagcatca acagccattt ttgcttcctt ttctgttgcc atgtgatatg
     2581 tggccagcac atgctcatga tcatgcggca ttacaatctt tccaaacttt cccgttgtta
     2641 tctctttccc tccgataatc aagggtattt caatccggct gctttccaat tccctcagct
     2701 cattggtgag caaaatacgc tcattgctcc cgggggcata attcaaaatc ttctcattgt
     2761 gcggattctc aaaatcaaaa atggcattat tcataatgtt ttgttttagg ttaatcaatc
     2821 gatatgcatc attttacata tcgggcacaa aattacattt tttaatatac tgccaagatt
     2881 tattatatca ggtaataaac tgctaaatat catatcgcaa ttcaggattc ggatttgttt
     2941 caggagacct gacaaattga cactttgaga gcattggcac atgctttgac agagggtcgg
     3001 ctgcaaaaaa cactatacca tgcagaaagg ttcaatcaat gtacagacgg gtaatatttt
     3061 ccccatcatc aaaaaattcc tgtattcgga acacgaaatt ttcctacgcg aattagtttc
     3121 aaatgccata gatgccacta ccaagctgaa aaagcttgta tcgttgggta aagccgatgt
     3181 tgagttgggc gacttaacga ttcaggtggc gctggatgcc aaaaaaaaga ccctgaaaat
     3241 tactgatcgc ggcataggaa tgaccgaaga ggaagtacaa aaatacatca acgaaattgc
     3301 cttcagtggg gctgaggatt ttgtaaaaaa atatgccggc aaagacgata aagaaatcag
     3361 cggaattatc ggacatttcg gacttggttt ttattcctct tttatggttt cggacaaagt
     3421 taccattgac actttgagtt atcaggatgg tgcgaaagct gtgaaatggg aatgtgacgg
     3481 ctcgcccgaa tacaccatga gtgaatctaa acgcaaagac cgtggtacgg aaatcacgct
     3541 gcatttgtcc gaagattcaa ttgaattcaa cgaagaatac cggattctcg acatgctgaa
     3601 aaaattctgc cgttttctgc cctacccgat tcagttcggc atggaaaaaa cgtcggaacc
     3661 catcgaggga gaaaaagaca aagacggtaa tcagaaatac gaagatgttg aaaaacctcg
     3721 tataatcaac aataccgacc cgctctggaa aaaaacgccg acagaactga aagacgagga
     3781 ttataaaaac ttctatcgcg aattgtatcc ctactcttat gatgagccgc tgttcaacat
     3841 tcatatcaat gttgactatc ccttcaatct gaccggtatt ctgtattttc cgaaactaaa
     3901 gaaaaacctt gaggtacagc gaaacaaaat tcagctatac agcaatcagg tttttgtaac
     3961 cgataatgtc gaaaatattg ttcctgattt tctcacgctg ctgcatggtg tgattgattc
     4021 gcccgacatt ccgctcaacg taagccgcag ctatcttcaa ggcgatccga atgtgaagaa
     4081 aatcagcagt catatcacaa aaaaagtcgc cgataaactt gaggaattgt ataaaaacag
     4141 ccgcgaagat ttcgaaaaga aatgggacga catccgtgta ttcatccgct atggcatgat
     4201 ttcgaccgaa tcctttgccg aaagagctga aaaatttgcc ttgctgaaag ataccgaagg
     4261 caactattgc accctcgagg aatacaaaga gaaaatcaaa gaagcacaaa ccgacaagga
     4321 tgacaagctc gtaattctgt acacaacaga caaagaagaa caacacacct acatttcggc
     4381 agcgcaggcc aaaggctatt cggttctcga aatggattct gtcattgata ttcattacat
     4441 cgattttctg gaacgcaaaa acgagaaaat tagctttaaa cgtgtcgatg cagaactgac
     4501 agacaagttg attgataaaa atgttgaaac cattgtcagc aagctgaacg aagatgaatc
     4561 aaaaaaagtg gaagaattgt tcaaaggcgc actgccggcc gatgtgcagg tatttgtcaa
     4621 agtagaagcc gctgccgaag attcaactcc ggtaagcatg atgcagcccg aatttatgcg
     4681 tcgcatgagc gatatgtcgc aattgggcgg catggattac atggccggca tgggtcgtag
     4741 ctacaccttg ctggtcaact ccaatcatcc ggtagtgaca ggcctggtgg attcgtctga
     4801 tgaagaaaaa aacaaaaacg ttgtcagcca gctcatcgac ctcgctttat tatcgcaggg
     4861 actgttgaaa ggtgaaaaac tttcaaggtt tatcaaccgg agtattgaca ttatcaaata
     4921 aatttttaat tttacttttc aaacaactca aaaacacatg gaaaatacaa cccagacctt
     4981 gccaggcggc tcacagccaa accgcagtaa tcccaaaccc aaaacgggat gcttaggatc
     5041 gaccatgata atcattctgc tgttgattat tcttcttccc atcggtatct ggatgtggcg
     5101 cgcctacaac cgcatggtta ccaaggatga attggttaca aaacaatggg ccaatgtcga
     5161 aaatgtgtat cagaagcgga tggatcttgt gcaaaacctt gtaaacacag tgaaaggcat
     5221 ggccgatttc gaaaaatcga ctctcaccga tgttatcgag gcccgaagca aagcttcatc
     5281 tatcaatata aacgccgata atctcgatga agaaaccttt gccaaattcc agcaggccca
     5341 ggatgaattc agcggcgcac tcagtcgtct tatggttgtt atggaacagt accctcaact
     5401 gcaggcagtt gaaggcttta aaaacctgat gaacgacctt aaaggcatcg aaaatgaaat
     5461 tcttgcacaa cgaaatgcct acaacgaagt tgctatggat tacaatcaat acatccggac
     5521 gttcccgcgc aacctgtttg ccagcttatt cggatttgat aaaaagggct atttcaaatc
     5581 gtcagccgga gctgaaaatg ctccaacggt cagctttgaa taaaataatt ctgcttccgg
     5641 atcccggcca tttgcgccgg gatttttttt gccaacctct ctgcgtcgat gcaatcgacc
     5701 tgagtgcatc ggcttgagta agggcttcca acaacgcaga gaggtcgctt cacgtgaaac
     5761 gtgacaaagt cgcgacgcac tcaggttgtg aaaaaatgat aaaaatcatt atgcggtttt
     5821 taataacaaa tcaatttttt atctttacca aaatttactt ttgtgaaaca aatccggcca
     5881 ttgcaaaaag tctggaaatt ttacgccgac ggcttccgca acatgccttc gtggggacgt
     5941 caggcctggg ctgtggtcat ttttaaagga atagtggttt tcatcatcat gaaatttgtt
     6001 ttttttccca accatctgaa aaaaaacttc gacaccgatg aacaacgcag cgaacacgtt
     6061 ttggatcaac taacaaaaac aaaataaagc atggatcaaa tctctaccct ggtcgattgg
     6121 tctcgggcgc aattcgcgct tacagccatg taccactgga ttttcgtccc gctcacactg
     6181 ggtatgacct tcatgattgc cttcatggaa accttgtatg tgcgcagcgg aaacgaagag
     6241 tggaagcgga ttacaaaatt ctgggccaaa ctgttcggaa tcaattttgc catcggcgtg
     6301 gctaccggaa ttattcttga atttgaattc ggaaccaact ggagcaacta cagctggttt
     6361 gtgggcgaca tctttggcgc tcctctggcc gtcgaaggca tcatggcttt ctttatggaa
     6421 agtactttta ttgccattat gttttttggc tggaacaagg tgagcaagaa atttcacctg
     6481 ctctcaacct ggctggtagc catcggcgcc aacctatccg ctttatggat tctggtagcc
     6541 aacggctgga tgcaattccc ggtaggcaca gttttcaatc ctgaaaccgc aagaaacgaa
     6601 atggttgatt tctgggcagt gcttttcagc cctgtggctg tcaacaaatt tcttcatacc
     6661 atatcgaatg gctttgtggt ttcagcactg tttataatag gcgtcagtgc ctggtttatt
     6721 ctgaaaaaac gcgaaattgt ttttgcgaag aaaagcatgc tgattgctgc aacatttgga
     6781 ttgctttcgt ccatatttct tgctcttacc ggtgatggct cggcttatca ggtagcgcaa
     6841 aagcagccca tgaagctagc cgccatggaa ggtttgtaca aaggtcaaaa agaagccgga
     6901 ctgattatgg tcggcatgat cacacccggg aaacaacccg gagacaacca agccgactat
     6961 gtgttcaaac ttgaaattcc aaaactgttg tcttttcttg gttatcgcga tgcaaaggca
     7021 tttgtgccgg gaattgatga tcttgtttac ggaaacgaag agcaggggct gatttcagca
     7081 aaggaaaaaa ttgagcgagg caccattgct gttgaaactt tacgcaaatt taaacaggcc
     7141 aaacaatcag gagatagtac aaaagcggca gaacttgcac ttcttttcga tcgaaaaacc
     7201 actgccggcc aggattttta caataattac ttccgttatt ttggctacgg ccatattgaa
     7261 aaaccggaag atatagtacc caatgtaccg cttgtttttt acagttttca catcatggtg
     7321 gcactcggat ttttcttcat tttctttttt gcagtcatgt tgtttttcct gatgaaaaat
     7381 tccgtcgaaa agcgaaaact atggctgcat ctggcaatat ggactttccc gctgggattc
     7441 attgcatcgc aggccggctg gattgtggcc gaagttggtc gtcagccctg ggtgattcag
     7501 gatctcatgc ccgtcaatac ggccgtttcg caggtcagcg catcggcggt gcagcttact
     7561 tttgttttgt ttgccatcat attcacttcg ttgctgatcg cggaaatttc catcatgacc
     7621 cgcgccatca aaaacggacc aaccgaagga ggaaaataat atgtttgaaa cattttcata
     7681 tctggctttg caggaatact ggtggttcat catttccatt cttggtgcct tccttgtgtt
     7741 tctgcttttt gttcagggcg gacaaagtct gctctacacc ataggaaaag aagaaccgca
     7801 aacatcaatg ctggtgaatt cgctcggccg caaatgggaa ttcaccttta ccacattagt
     7861 aactttcggt ggtgcttttt tcgcatcatt tccattgttt tactccacga gttttggtgg
     7921 tggatactgg ctctggatgt tgattctgtt tacattcatc ttgcaagccg tcagttacga
     7981 attccgctcg aaacaaggca atgtattcgg taaaaaaacc ttcgaatggt ttctgtttct
     8041 gaatggattt attggaaccg tttcgctcgg agtggccgtg gctactatgt ttaccggggg
     8101 taactttgtt cacgatgata tgaatttcac ttcgtggagc aacaactggc acggcctcga
     8161 agcatttgcc aaccccatca atgtggcgct gggactggcg gtattcttcc tgtcgcgtgt
     8221 gctggccatt ctgtatttca acagcaccat caacgatggt attatccgcg aacggaacaa
     8281 aaaacacctg ctcatcaatt cggtgctttt cctgatcttt ttccttgcct ttgttattgc
     8341 catctggctc atcgacggct acgcctacaa tcctgccacc ggcgaaattt ttaccgagcc
     8401 acacaaatac ttctacaata atatagaaca tgcgctcaca ggaatccttt ttctgctggg
     8461 tgttttaatg gtcttgttag gtttgttccg cggcaacacc tgttataaaa gctgcagtgg
     8521 taaagcattg tatccggtcg gaatcgggac atttctcacc gtgctttcgc tattctgcaa
     8581 tctgggttat aacaacaccg ttttctaccc ttcgctcagc agcatgcaga gcggacttac
     8641 gattgaaaaa gcttcatcga gccagtttac gctgaccgta atgagttatg tatcgctgct
     8701 ggtgcccttt gtgctggcat acatcattta cgcatggcat gcactgaata aaaagaaaat
     8761 cgatgttgac gaaatgaacg acggatcaca caaatattaa aaacagaaaa ccatgtacat
     8821 tatttcaata ttgtggttgc tcacctggcc tttgctgatc tttgtgtcgt atcagaccat
     8881 ccgcctgttg ttgaaaaagt acggcgataa gctggagaag tcagaataag ccgctatgcg
     8941 aagtctctcc ccgctatgcg aagtatatac ttcgcatagc ttgtcaatac ttcgcacagt
     9001 aaatgttatt tagatgttaa ttatttgttt ttaaataatt tattgcaata tctttgtgat
     9061 atcaatttaa tatcaaatgg aaaagaacat caaaccgtcg cttgggtatc tggcactcgc
     9121 catcagcatt cttatgctcc tatcaccctt tctcattttt gcagatgaaa gcaatctgct
     9181 gatcatcacc ggcaccatcg tactgtcatt tctgtcgatt tttggcttca tcggcctgtt
     9241 tgtcgtcaat ccaaatgaaa gcatggttct gattcttttt ggaaaatata tgggaaccgc
     9301 ccgcgaaaac ggcttctttt gggtaaatcc tttttatgtc aagaaaaaga tttcgcttcg
     9361 tgcacgcaat atggaaagcg agtcgctgaa agtgaacgac aaaatgggaa atcctattat
     9421 tatcagtgtt gtgatggtct ggcgtatccg cgatacgtac aaagccgctt tcgaagtcga
     9481 taattacgaa atatttgtaa agacgcagac cgaagctgct gtacgcaaac tggcccacac
     9541 ctatccttat gacaccttcg acgatgcaca ggaagaaatt tcactgcgcg gcggcggtga
     9601 cgaaatcaat gccgagctgg aacgcgaaat ttcggaacgc cttgcgattg ctggtgttga
     9661 agtacttgag tcacgtcttt caaatctttc atatgcaccc gaaattgccg gcgccatgtt
     9721 gcagcgtcag caagctacag ccatcattgc agcccgtaca cgtattgtcg aaggtgccgt
     9781 aagtatggtc gatatggcgc tgaatcagct cagcaagaaa ggcatagtcg aactcgacgc
     9841 agaacgcaaa gccgccatgg tgggcaatct gatggttgta ttaacttcgg aacgcgctgc
     9901 tcaaccgatt gtaaatgcag gaactttgta ttaaaatcaa acagccatga caactttcag
     9961 cgaaacccag catacgcaaa aacctttgtt tatcgccatc cctattgttt tgtggctgac
    10021 tttcatggtg tttgcagtta ttgaattgta caattatctc catttcggaa tacctctggg
    10081 cgacaaaggc gaagaaattc cattgggggg ttttattggc attatgattc ttctgagtct
    10141 ggttttcgct ttttttctgt ttatggtatt acgcctgcaa ttaaaaattt caatcagcga
    10201 aagcggtatt tattttgaaa tgccgccctt taaaaagccg gcaactttta aacccgagga
    10261 aattgaatcg gcttttgtgc gtaaatacta ccctatccgt caatatggcg gctggggctg
    10321 gcgcggaata aaagataagg cctacaatgt gtcgggccgc tggggaattc agattgttct
    10381 aaaaaacaaa aaacggattc tgcttggcat tcaaaaccac gaagatgcag cacgcgcgct
    10441 cgaatcaatt ggcttcaaca aacaacttta gtcatgggta aaaaagcatt tgcactccgc
    10501 attgacgaag aggtgatgaa ggccctggaa aaatgggcgg ccgatgagtt ccgctccgtc
    10561 aacggacaga ttgaatggat actgcatcag gcgatggcca aggccggtcg tgctccgaaa
    10621 cagaagaact ctgaacaccg aacaaggaaa gatgaaggat gaaatgaggt ttggaggatg
    10681 aagtgaagtt tgaagaaaaa tttcatttta ccagacactc tgttcattag tcgtggtcaa
    10741 aaaaatcgta tttcgttttt ttgaaacgtc gcttatattc tcacatatga cgaagtcgtc
    10801 acatttcctc attatttcat atttcgtatt tcgtatttcg tatttcgtat ttcgtatttc
    10861 gtatttcgta tttc
//
LOCUS       DAOPKE010000092        10542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6615_length_10542_cov_13.488986, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000092 DAOPKE010000000
VERSION     DAOPKE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6615_length_10542_cov_13.488986"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..1332)
                     /locus_tag="PKY63_10375"
     CDS             complement(370..1332)
                     /locus_tag="PKY63_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPF01062.1"
                     /translation="MKKILVIGAVGQIGSELTMALRNEFGNDNVIASTRKTPPTDTVK
                     NSGPFEFFDIVDRPRMTEVCKKYNVDAIVNMAAILSAVGEQNPMMAWDINMNGLINVL
                     EVAREMNMKQVLVPSSIAVFGPGTPLDCAPQETVLKPTTMYGVTKVAGELLADYYVRK
                     YNLDVRGLRYPGIISYETLPGGGTTDYAVGIYFDAIKHGKYTCFVKEDTRLPMMYMPD
                     CLKATIDLMKADFSKLKHHSDFNVGAMSFSAGELAASIKKYIPSFEIEYKPDFRQNIA
                     DSWPNAVDDKAAREEWGWKPAFDLDAMTQDMLKNIKIKHEKGLI"
     gene            1511..2758
                     /gene="kbl"
                     /locus_tag="PKY63_10380"
     CDS             1511..2758
                     /gene="kbl"
                     /locus_tag="PKY63_10380"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPF01063.1"
                     /translation="MHKTIVEHLNKELDTIREAGLFKTERIIESEQGAEIVVKGKKVL
                     NFCANNYLGLSSHPELIKAAHEEIDHRGYGLSSVRFICGTQDIHKVLEKKVSDFLGME
                     DTILFSSCFDANGAVFEPLLGENDAIISDALNHASIIDGVRLCKAQRWAYKHSDMRDV
                     EEVDPATGKMAKGLERCLKEAREKGCRFIMIATDGSFSMDGDIAVLDKICDVAEKYDA
                     MVMVDDSHSTGFLGKTGRGTHEYRGVMDRVDIITTTFGKALGGASGGCISGPKAIIDW
                     MRNKARPYLFSNTVAPSVVGATIKVMDLLTASTELRDKLEANTKLFRTKMTEAGFDIV
                     PGDHPITPIMFGKYPDCSKLAVKFADRMLEEGIYVIAFSFPVVPKGKDRIRVQISAGH
                     STEQIEKAIAAFTKVGKELGMIK"
     gene            3220..3909
                     /locus_tag="PKY63_10385"
     CDS             3220..3909
                     /locus_tag="PKY63_10385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPF01064.1"
                     /translation="MRFVPEKSYRRRICLSLSGKTFSECQQQLGDAPFAELRLDLIEM
                     NAIEIRQLIDCGLEWIIAVRAPFVEKQNYAELFDAALTGNLRFVDFDFGIIQDPHVQA
                     LLNKSREAGFKIMFSWHDFEKTPDRDFLIKKRNEIFEADPDAIKMVCKGNNIEDAGVI
                     LFAYRNFKNITAFCLGEECCETRITAMKWGIGISYAHPDGAESTAPGQFSYSDMLAFG
                     EILDRKGEFNE"
     gene            3902..5149
                     /gene="aroA"
                     /locus_tag="PKY63_10390"
     CDS             3902..5149
                     /gene="aroA"
                     /locus_tag="PKY63_10390"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPF01065.1"
                     /translation="MNEVVHPYRIEGVLQAVASKSYLQRALAIAGLADGPTFIRNYNA
                     SADAIAAQNIITKLGSKVRGGVRMHIVPGKPDFSSDIMLDADESGLSLRMFSFIAALY
                     GRAIQVTGKKSLLERPVEPLVEALLHCGVQVKTSNGALPLTICGPLAKNKIELDGSFS
                     SHMLSGLLIAAPLLEHDTEISVRNLVSRPYVAMTLNIMRQFGVTVENNNFCQFYIKGR
                     QQYQGQEYAIEGDWSAAANFLAGAAVSGKIEMTGLNQNSLQADIKIREIIVDFGAHVN
                     LLNNFISVSKNEAKPFNADLTDCPDLFPPVVVLAAAANGKSVLTGTQRLIHKESNRFK
                     SLLTVFSRLGLKMEPHGNELHIYGTGKLTGGEVDSFNDHRIAMCTAIAGTLASSEIRI
                     SNADAVSKSYPEFFQDLRKVMIK"
     gene            5194..6390
                     /locus_tag="PKY63_10395"
     CDS             5194..6390
                     /locus_tag="PKY63_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF01066.1"
                     /translation="MGVINDNCMKGLLTLVIGLVAILSNAQNQNVTAADTLFYKPQLA
                     SLLDLVDTNRILSDVSVLSSIYSRDVRHPGNDTAAIFIADKMQQYNVSIIQQEYYFEF
                     DTLAPNVNVIGIKTGEQYPDSFIIVSAHFDSYSNSENAPGADDNASGVAGMLEILRII
                     DTVKTKYSIMFCAFNAEEWGWQGSTMFVDSAIANGLAIKFDLNIDMIGYSNPVDSHYI
                     VLCTGFSYNYLAEKYRDVAAMYVPLLSIPGNFSNAYYYSDQKSFIDNYIPALSFYENY
                     FDNNPFIHTAADTIGAGFNSLTNAKLITQANLAFIIEFAGINAYTDIEKHEMPEVKIS
                     PNPVVDGFFIKGIKSGTRWSVVDVSGRIVMQGFQNNKVVDVSTLIPGVYLMKTENSQC
                     YFIKAN"
     gene            complement(6399..7058)
                     /locus_tag="PKY63_10400"
     CDS             complement(6399..7058)
                     /locus_tag="PKY63_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPF01067.1"
                     /translation="MCGRFTANVKKKDLEEEWNLAVPPEFRPRYNIAPSQVVGVITNK
                     QPSDLSLFRWGLIPSWAKDNSMASHMINARVETLNEKPSFRKLAQTARCLIPATSWFE
                     WTQDKSRQPFAIKLKNAKLFAMAGLWDTWIDANGQKVNTFTIITTEAAENIRHIHERM
                     PLVVPKEQQHDWLTNKADAATIAAKSGQLEFDFFPVSSRVNNPKNNDESLLDEFRETL
                     L"
     gene            7137..7451
                     /locus_tag="PKY63_10405"
     CDS             7137..7451
                     /locus_tag="PKY63_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01068.1"
                     /translation="MDEKVENISVADFSKHMFWDVNKAEIDLMRDRHYVVRQVFSCGL
                     MNDWRLICRLYGIPEIGEIAKKIRDLDPKSHAFIALIAKIPEEDFLCYTTKQSTPQHW
                     NF"
     gene            7406..8026
                     /locus_tag="PKY63_10410"
     CDS             7406..8026
                     /locus_tag="PKY63_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF01069.1"
                     /translation="MLHYKTIDTPTLELLKQLMQIPALEKLRLVGGTGLALQKGHRKS
                     VDIDLFGEIEADDIELMNSLSVFNDLKTIHQTKNIKVFSITNIKTDIVNYPYPWLFPV
                     IETNGIRLADIRDVAAMKVAAITNRGTKKDFIDLYFLLKDFTVGDIVNFYLAKYNDGS
                     EFLALRSLVYFADAENDLMPEMLMPAEWESVKLKIKTEVEAFQGKA"
     gene            8207..10408
                     /locus_tag="PKY63_10415"
     CDS             8207..10408
                     /locus_tag="PKY63_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF01070.1"
                     /translation="MKNIFICLLFLFSGLYLTAQNPAELNKQFEFSADSKAQPTFNLD
                     SLITACLSDISADSIEHYMRELQGFGTRFLMAPNRRHIAEFLAGKFESFGYTDVELDS
                     FCYVSGTYPYDSLWQYNVIATLPGSGNNATQYLTGGHYDCIIYNLQPYDSTTFVPGAD
                     DNGSGTSATLEIARVIALNNIHPDATLKFVCFAAEEYGLLGSEHYTNSARLNNEDIGF
                     YVNMDMIANNIIGDSLVNLYHYENAEYVYNVVMNVCQAYTYLTPVMIEGNSPGSDSYS
                     FWENDYEATFLIESDFSSYYHSAGDTVGNCNMHYCAEIASASCGALLIGAFSPRPVQY
                     FRMVNFNNCMDALLTWLPSGDQNVAGYNIYTGIVPGVYDSVVFVTDTFLILDNLVQGN
                     TYFSAVSVVNTNGFESMLREDSMTSVYCGFDEGVLIVDGSEGGLSNPTDETIDQFYMD
                     VLYGFQTTEYAADSIGSIDLTTLGRYSSILWHINSAASATSLAQSVEDLENYLSCGGN
                     LLLTAYRPEILIGENTYSGLSVHNSGSFFARFLKVKSSQNSIYSYMSGAVPEIVLFDT
                     LHCDSSKTGGMMKFVEVLNPTPDAAGIYAYDSPYDTTHPISELKYENVGVLYDGSDFK
                     TLTLGFPLYYMNKDEVQSMLRYVFENIFGEAYTSIEENATASTLQCYPNPVSDILYVK
                     GAQGNTFTILDASGRIVRSGKTTSNVIDVSSLKSGVYTLRNESSAILFIKE"
BASE COUNT         2976 a         1993 c         2395 g         3178 t
ORIGIN      
        1 ttaaatgact aaatgattta aatgactaaa tggttaaatg attaaatgaa tgattggatg
       61 caactccgcg ttggtatatg caaacctgtc tgctgcaggt cggtccgcgt ctaaaaaaga
      121 tttcatttgt gtaaatctgc gaaaatccgc gtctaaaaaa gacaaaaaag cgccgtgctt
      181 tcgcacagcg cctttatttt ttttgaagtt ctattttgaa gtttataaaa tcatcatgtt
      241 tataaagtaa aaagctcctt cataaatttc tgcttttact tcattcttcc ttgttcgatg
      301 ttcagtattc atgatttgat gctcacttca tccttcagat caacaatcac aaatcaccga
      361 tcgatattct taaatcaatc ccttctcatg tttgattttg atatttttga gcatatcctg
      421 cgtcatggca tcgaggtcaa aagcgggttt ccagccccat tcttcgcggg cagctttgtc
      481 gtcaacagca ttgggccatg aatcggcaat gttctggcgg aagtcgggtt tatactcgat
      541 ttcgaaagag gggatatatt ttttgatgct ggctgcgagt tcgcctgcac tgaaactcat
      601 ggcgccgaca ttgaaatcgc tgtgatgttt cagtttgctg aaatcagctt tcatcaggtc
      661 gatggtggct ttcagacagt cgggcatata catcatcggc aggcgggtgt cttctttaac
      721 aaagcaggtg tatttgccat gtttgatggc gtcgaaatag ataccaacag cgtaatccgt
      781 ggttccaccg ccgggcaagg tttcgtagct gatgatacca gggtagcgaa gaccacggac
      841 atcgagattg tattttctta cataataatc ggcaagaagt tcaccggcta ccttagtaac
      901 accgtacata gtggttggtt tgagaacggt ttcctgtggt gcacaatcga gcggggtgcc
      961 agggccgaaa acagcgatcg agctgggaac gagcacctgt ttcatattca tttcgcgggc
     1021 aacttcgagc acgttaatca gaccgttcat attgatatcc caggccatca tcggattttg
     1081 ttctccgaca gctgaaagaa tggcggccat gtttacgatg gcgtccacat tgtatttttt
     1141 gcagacctcg gtcattcgcg gccggtcaac aatgtcgaaa aattcgaaag gtcccgaatt
     1201 cttaacagta tcggttggag gtgttttacg ggtacttgca attacgttgt cattgccaaa
     1261 ttcattgcgg agagccattg tcagctcaga gccaatttgt ccgacggcgc cgattaccag
     1321 gatttttttc atatttcttt ggtttgattt gttttattaa cagggagcaa aaatagttaa
     1381 aaaatcattt gaccgctgca gcggaaattg ttgtattgca agttttttta tgacataaaa
     1441 aagatgaaca tttttatgat agaattcgtg aaacttatat ctttgccatc tgaaatttaa
     1501 aaacagacca atgcataaaa caattgttga acatctgaac aaagaactcg acaccattcg
     1561 tgaagccggg ttattcaaaa ctgaacgcat tattgaaagc gaacagggag ccgaaatcgt
     1621 agtaaagggc aagaaagtgt tgaatttttg cgccaataac tatttgggac tttcatcaca
     1681 tcccgaactg attaaagctg ctcatgagga aatcgatcac cgtggttacg gactttcttc
     1741 ggttcgtttc atttgtggaa cacaggatat ccataaagtt cttgagaaaa aggtttctga
     1801 cttcctcgga atggaagaca ccattctttt ctcatcatgc ttcgatgcta atggagctgt
     1861 gttcgaaccg ctgcttggcg aaaacgacgc tatcatcagc gatgcgttga atcatgcttc
     1921 aatcattgat ggtgtacgcc tttgcaaagc tcagcgctgg gcttacaaac acagcgacat
     1981 gcgcgatgtg gaagaagtag atcctgctac cggaaaaatg gctaaaggtc tggaacgttg
     2041 tctgaaagaa gctcgtgaaa aaggttgccg cttcattatg attgctaccg atggttcttt
     2101 cagtatggac ggggacattg ctgttctgga taaaatctgt gatgtggccg aaaaatatga
     2161 tgccatggta atggtagatg acagccattc aaccggattc cttggaaaaa ccggacgtgg
     2221 aactcacgaa taccgtggtg ttatggatcg tgttgatatt attaccacga cctttggaaa
     2281 agccctcggt ggtgcttccg gcggttgcat cagtggtccg aaagccatta ttgactggat
     2341 gcgcaataaa gctcgtccgt atctgttctc aaacactgtc gctccgtcag tggttggcgc
     2401 aaccattaaa gtgatggatt tgctcacagc ttcgacagaa ttgcgcgaca aactggaagc
     2461 caataccaag ctgttccgca ctaaaatgac cgaagcaggt tttgatattg tacccggcga
     2521 tcatccgatt actccgatta tgtttggtaa atatcctgat tgttccaaac tggcagtgaa
     2581 atttgccgat cgcatgctcg aagaagggat ttatgtgatt gctttttcgt tcccggttgt
     2641 tcccaaagga aaagatcgta tccgcgttca gatttcagct ggtcacagca ctgagcagat
     2701 cgaaaaggcc attgctgctt tcaccaaggt tggcaaagag cttggaatga ttaaataatt
     2761 gttgcactgc agctaattta ttgtaagtaa agggtaaaag ccggagcaat ccggcttttt
     2821 tcttgcacct gaatgttgtc acgatggcac taagacacgg aaatcatttc acctgacttc
     2881 gccattggga cacctatgtt ttttttctga agttttttag gaaactcatc caataaaaac
     2941 aaaggaagcg tgtaaacact tattatgaat ttttactgac ccgccactgg cgggtcaaac
     3001 aataatagcc cggccattct ggccgggcaa aagatagaac aaaaaccgac ctcggagagg
     3061 tcgaataaaa cgaaaatatt gaaacaatgt ttttgtgctt ttttacgtgg ttgcagtcag
     3121 gagatgcgct cccaatgtag ctgccgcgag aattgccaat agggaataaa aatctccaat
     3181 gcttttgcag ttcaaaaata aattatgtac ttttgagtca tgagatttgt tccggaaaaa
     3241 tcatatcgcc gacgtatttg tctttcttta tccggaaaga ctttcagcga gtgtcagcag
     3301 caattgggcg atgctccgtt tgcagagttg cgtctggatt tgattgagat gaatgcaatt
     3361 gaaatcagac aactgattga ttgtggactt gagtggatta tagctgttag agccccattt
     3421 gtagaaaagc aaaattatgc agaactattt gatgccgcat tgaccggaaa tctccgcttt
     3481 gttgattttg attttggtat aattcaggat cctcatgtgc aggcattgct gaataaatca
     3541 cgggaagcgg gatttaaaat aatgttttca tggcatgatt ttgaaaagac acccgacagg
     3601 gattttctta ttaaaaaacg aaatgaaata tttgaagctg atcccgatgc aataaaaatg
     3661 gtctgcaaag gaaacaatat agaagatgcc ggcgttattt tatttgcata tcgcaatttt
     3721 aaaaatatta cagcgttttg tctgggtgaa gaatgttgcg aaacacgcat aacggccatg
     3781 aagtggggaa ttggaatttc ctacgcgcat cccgatggtg ccgaaagcac cgccccaggc
     3841 cagttttcgt attctgacat gctcgctttt ggcgaaatat tggacaggaa aggagaattc
     3901 aatgaatgag gttgtgcatc cttacaggat tgaaggcgtg ttgcaggccg tagcttcgaa
     3961 aagttatcta cagcgtgcat tggcaattgc aggtttggcc gatgggccaa cgtttatccg
     4021 aaattacaat gcctcggcag atgccattgc tgcgcaaaat attataacaa aactcggatc
     4081 aaaagtacgt ggtggagttc gcatgcatat tgtgccaggc aaacctgatt tctcatcaga
     4141 cattatgttg gatgccgatg aatcaggatt aagtcttcga atgttttcgt ttattgcagc
     4201 attgtatgga agggcaattc aagtaaccgg gaaaaaatcg cttctggagc ggcctgtaga
     4261 gccgctggtt gaagctcttc ttcattgcgg cgtgcaggtg aagacttcca atggcgcatt
     4321 accacttacg atttgcggtc ctcttgcgaa aaataaaatt gagctggatg gttcttttag
     4381 ttctcacatg ctttcaggat tgctgattgc agctccattg cttgaacatg acaccgaaat
     4441 ttctgtgcga aatctggtga gccggccata tgttgccatg actttaaata tcatgcggca
     4501 gtttggagtg acagttgaaa ataataattt ctgtcagttc tatatcaaag gccgtcagca
     4561 gtatcaggga caggaatacg ccattgaagg cgactggagt gccgctgcca attttcttgc
     4621 aggcgcagct gtgtccggaa aaattgaaat gaccgggctg aatcagaatt ctttgcaggc
     4681 ggatattaaa atccgtgaaa ttattgttga ttttggggct catgtaaatt tgctcaataa
     4741 ttttatcagt gtcagtaaaa acgaagcaaa gccattcaat gctgacctga ccgattgtcc
     4801 cgacttgttt cctccggtgg tggttctggc tgctgctgca aatggcaaat cagtgcttac
     4861 tggtactcag cgattgattc acaaggagtc gaatcgcttt aaatcgttgc tgactgtttt
     4921 ttcgagactt ggattaaaaa tggagccgca cggaaacgag cttcacatct atggtacagg
     4981 aaagctgacc ggaggcgaag ttgatagttt caacgatcat cggattgcca tgtgcacggc
     5041 cattgcaggg actctggcca gctccgaaat acgcatcagt aatgctgatg cagtcagtaa
     5101 atcgtatccg gaattttttc aggatctgag aaaagtgatg ataaaataat tgtcattact
     5161 gatcctttaa ttattgtgtg attaaaaaga ataatgggtg ttattaacga taattgtatg
     5221 aagggattgc tcacattggt tattggattg gtagctattc ttagtaatgc ccaaaaccaa
     5281 aatgtaactg ctgccgatac actgttttat aaacctcaat tggcatcttt gcttgatttg
     5341 gttgatacca acaggatttt atcagatgtt agtgttctgt cttccatata tagccgtgat
     5401 gtacgacatc cgggaaatga tacggctgca atatttattg cggataaaat gcaacaatat
     5461 aatgtttcaa ttattcaaca ggaatattat tttgaatttg atactttggc accgaatgtt
     5521 aatgtaatag gaattaaaac cggggagcaa tatccggatt catttataat tgtaagtgct
     5581 cattttgaca gctacagtaa tagtgaaaat gctccggggg ctgatgataa tgcttcgggt
     5641 gtggcaggaa tgcttgaaat actaagaatc atagataccg ttaaaacaaa gtactcaatc
     5701 atgttttgcg catttaatgc tgaagaatgg ggatggcagg gaagcacaat gtttgttgat
     5761 tctgcaattg caaatggttt ggcgataaaa ttcgacctta atattgacat gattggttac
     5821 tccaatccgg tagattcaca ttacattgtt ctttgcacgg gtttttctta taattatctt
     5881 gcagaaaaat atcgggatgt tgctgccatg tacgttccac tattatcaat tcctggtaat
     5941 ttttctaatg cctattatta tagtgatcaa aaaagcttta tagataacta tattccggca
     6001 ttatcctttt atgagaatta ttttgataat aatccattta ttcatactgc agcagataca
     6061 attggcgcag ggtttaattc attaacaaac gccaaattaa tcactcaggc aaacctggct
     6121 tttattattg aatttgccgg gataaacgcg tatacagata ttgaaaaaca cgaaatgcca
     6181 gaggtgaaaa tttcccccaa tccggtagtg gatggttttt ttattaaagg aattaaatcc
     6241 ggaacccgat ggtctgttgt tgatgtcagt ggcaggattg taatgcaggg ttttcagaac
     6301 aataaggtgg ttgatgtgtc cacattgata cccggagttt atttaatgaa aacagaaaat
     6361 tcgcaatgct attttattaa ggcaaattaa ttaagtgttt aaagcagtgt ctctctaaac
     6421 tcatcgagca agctttcatc gttatttttc ggattgttca cccgggatga aaccggaaaa
     6481 aaatcaaatt caagctgacc tgatttcgcg gcgatggtgg ctgcatcagc tttgtttgta
     6541 agccagtcgt gctgctgttc tttgggaact accagcggca tacgttcgtg tatatgacga
     6601 atgttttctg cagcttcggt agtaatgatg gtaaacgtgt tcaccttttg tccgttcgca
     6661 tcaatccagg tatcccagag tccggccatg gcaaatagtt ttgcgttttt cagctttatt
     6721 gcaaatggct gacggctttt gtcctgtgtc cattcaaacc acgatgtggc gggaataagg
     6781 cagcgggcgg tctgcgcaag tttgcggaac gatggttttt cgttcagcgt ttcaacgcgt
     6841 gcattgatca tgtgcgatgc catggaattg tctttggccc atgatggaat caggccccag
     6901 cgaaacagcg aaagatcgga tggctgtttg ttggtgatga ctcctactac ctgcgaaggt
     6961 gcaatattat agcgcggcct gaactcgggt ggtacagcca gattccactc ctcttccaga
     7021 tcttttttct ttacattggc tgtgaatcgt ccgcacatga tgtgtggttt ttgtagatac
     7081 gaagataagg aataaatgcg atttttgtat ctttgtacat attcacaatg agcgcaatgg
     7141 acgaaaaagt cgaaaatatt tcagttgcag atttctcaaa gcatatgttt tgggatgtga
     7201 ataaagcaga aattgattta atgcgcgatc gacattatgt cgtcaggcag gtgttcagtt
     7261 gcggcctgat gaatgactgg aggcttattt gccggttgta tggcattccg gaaatcggtg
     7321 aaattgcaaa aaaaatcaga gaccttgatc caaaatcgca tgcttttatt gcattgatag
     7381 ccaaaatacc tgaagaggat tttctatgct acactacaaa acaatcgaca ccccaacact
     7441 ggaacttcta aagcaactga tgcagattcc ggctctggaa aagctacgtc tggttggcgg
     7501 aacaggactg gctttgcaaa aaggtcaccg taagtctgtt gatattgatt tgtttggaga
     7561 gattgaagcc gatgatattg agttgatgaa ttcactcagt gtttttaatg atttgaagac
     7621 cattcatcaa acaaaaaaca ttaaagtttt ttccattaca aatatcaaga cagatattgt
     7681 gaattatccc tatccatggt tgtttccggt aattgaaacg aatggaatca ggttggctga
     7741 tatcagagat gttgctgcaa tgaaagtggc agccattaca aatcgcggta cgaaaaagga
     7801 ttttattgat ttatactttt tgctcaagga ttttactgtt ggtgatatag taaatttcta
     7861 tttggcgaaa tataacgatg gctctgaatt tttggcgctg agaagtcttg tttattttgc
     7921 tgatgctgaa aatgatctga tgcctgaaat gttgatgcct gcagaatggg aatccgttaa
     7981 attgaaaata aaaaccgaag ttgaagcttt tcaagggaaa gcataacaat cttttttggt
     8041 ttcagtctat tcgattaatg caattagacc aaatttgaaa taaggtttag aagtaagata
     8101 tttcaaatcg gtcaaatatt tccttttact tgtttatatt tacctttaac ttgttggttg
     8161 attcaggttc ttattttgat tcaataaatt ggcaaaaatt cgaataatga aaaacatttt
     8221 tatctgtctg ttgtttttat tttcaggatt gtatctaact gctcagaatc cggcagaact
     8281 gaataaacaa tttgagttca gcgctgattc taaagcgcaa ccgacattca atcttgattc
     8341 attgattacc gcatgtctgt cggatatcag tgccgattca attgagcatt atatgcgcga
     8401 gctgcaaggt tttggcaccc ggtttctgat ggcaccaaac cgcagacata ttgcggagtt
     8461 cctggcaggg aaatttgaat catttggtta tactgatgtt gaacttgatt ctttctgtta
     8521 tgtttcgggc acgtatccat acgattcttt gtggcagtat aatgtgatcg caactttgcc
     8581 gggctcggga aacaatgcaa cacagtatct gacaggaggt cactacgatt gtattatcta
     8641 caatttgcaa ccctacgatt cgaccacttt tgttccgggc gccgatgaca atggatcagg
     8701 aacctctgca accctcgaaa ttgcacgtgt aattgcgctc aacaatattc atccggacgc
     8761 cacattgaag tttgtttgtt ttgctgccga agaatacggc ttacttggca gcgaacatta
     8821 cacaaactca gcccggctga acaacgagga tatcgggttt tatgtgaata tggatatgat
     8881 tgcaaataat ataataggag actcgctggt caatttgtat cattatgaaa atgctgagta
     8941 tgtttacaat gttgttatga atgtttgtca ggcttatacc tatctcacgc ccgtaatgat
     9001 tgaaggaaat tcacctggct cagacagtta ttctttctgg gaaaatgatt atgaagctac
     9061 atttctgatt gaatctgatt tcagctccta ttaccattca gcgggtgata cagttggtaa
     9121 ttgcaacatg cactattgtg ccgaaattgc cagtgcttcg tgcggggcac tattgatagg
     9181 tgctttttcc ccaaggccgg tacagtactt cagaatggta aatttcaata attgcatgga
     9241 tgctttgctt acctggctgc cgtctggtga tcagaatgtt gcaggataca atatctatac
     9301 tggaattgtg ccgggtgttt atgattccgt cgtttttgtg accgacacct ttttaattct
     9361 tgataatttg gtgcagggaa acacgtactt ttcagcggtt tcagtagtaa atacgaatgg
     9421 cttcgagagc atgctacgcg aagattcgat gacttcggtc tattgcggtt ttgatgaagg
     9481 agtattgatt gttgacggct cggaaggagg tcttagcaat ccgacagatg aaacaatcga
     9541 tcagttttac atggatgttc tgtatggttt tcaaacgact gaatatgctg ccgacagcat
     9601 cggtagcatc gatctgacaa cgctgggccg gtattcttcg atactatggc atatcaattc
     9661 cgcagcgtca gcaacctcgc tggctcaatc ggtcgaagat ctggaaaact atttgtcctg
     9721 cggaggaaat ttgctgttaa cggcttacag acccgaaatt ctgatcggag aaaacacgta
     9781 cagcggattg agtgttcaca atagcggaag tttttttgcc agattcctta aagtgaaaag
     9841 ctctcaaaac agtatttatt cctatatgag cggggctgtt cccgaaatag ttctttttga
     9901 tactttgcac tgcgattcct caaaaaccgg aggaatgatg aagtttgtcg aagtattgaa
     9961 ccccacacca gacgcagccg gaatttacgc gtacgattca ccttatgaca caactcatcc
    10021 aatcagtgaa ctgaaatatg aaaacgtcgg ggtattatac gatggcagcg atttcaaaac
    10081 acttacactc ggattcccgc tctattacat gaataaagat gaagttcaat cgatgctgcg
    10141 atatgtgttt gaaaatattt tcggtgaagc atataccagt atcgaagaaa atgcgacagc
    10201 atcaacgctg caatgttatc cgaatcctgt aagcgatata ttgtatgtaa aaggcgcaca
    10261 gggaaacact ttcacgatat tggatgcgag tggccgtatt gttcggtcgg gaaaaacaac
    10321 cagcaatgta atagatgtca gttcgctgaa atctggcgtt tacactctgc ggaacgaatc
    10381 ctcagctatt ttgtttatta aggaataaaa ttgatcatat caaccccgtc caggaatacc
    10441 tgctgatgac gttgccgaat ttggtttgca ggaactggaa ggaagggagg aaatgtttgc
    10501 gttcgagata gacgaaatat tcgcgggtga gcacatgaat at
//
LOCUS       DAOPKE010000093        10471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6670_length_10471_cov_13.990303, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000093 DAOPKE010000000
VERSION     DAOPKE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6670_length_10471_cov_13.990303"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..640
                     /locus_tag="PKY63_10420"
     CDS             449..640
                     /locus_tag="PKY63_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01071.1"
                     /translation="MAAPDDNFVKQNNYNFALEEEFDYLDELSRELEEQRKTAEKTTP
                     MTIKENGFEENESSKPLKE"
     gene            complement(649..2166)
                     /locus_tag="PKY63_10425"
     CDS             complement(649..2166)
                     /locus_tag="PKY63_10425"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPF01072.1"
                     /translation="MEKSFQLCPNPLVKFLKKTPQEFSVDDIIAFVAESKIQMLNFHY
                     TGGDGRIKTLNFVVSGEEQLRQLLLSGERVDGSSLFPFMESGSSDLYVVPKFRTAFIN
                     PFSEIPTLGIFCRYFDKDGNPMEMSPENILLRAHENLVNKTGYSMHAMGELEYYVISE
                     REDLFEAVDQKGYHESVPFTKWEHLRKEAMLYIAQMGGKIKYGHSEVGNFSDEFYNYE
                     QNEIEFLPTDIEEAAEQLILGKWILRSLAYKYGVSLSFAPKITVGKAGSGLHIHMKLL
                     KDGVSVTSDENGLTDVARKAIAGIMDLSASLTAFGNTVPTSYLRLVPHQEAPTNICWG
                     DRNRSVLVRVPLGWAGAASKMTAIANPGNKEATLDTREKQTFEFRCPDGSADIYLLLA
                     GLVTAATHGLTDKDALQKANDTYVNVNIFHEEHKAVAEKLKQLPICCADSATALEKQK
                     DFYLESGVFPPRVLDSLIKKLNSYDDSGLSERLFALPDDQRIKSIGDLVQKYLNC"
     gene            complement(2360..3607)
                     /locus_tag="PKY63_10430"
     CDS             complement(2360..3607)
                     /locus_tag="PKY63_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF01073.1"
                     /translation="MLFLRLIKESFLFAISAILVNRLRTILTVLGITIGIFSVIIVFT
                     VVDSMKNQILSSIESMGTNVVFVQKWPWSFSKDFEWWEYAKRPQPVIEDLKALEKNAS
                     TIESAAIMSSAMSNVEFGDSKFESVGIGGISEAYNKVFVFDIARGRYFSPSEFNNGVA
                     VCLLGATIAENLFGVNDPIGQYVKFLNKKVKVIGVLKKEGTNNIGNSNDEVVMVPFNF
                     IRQVIDVDDEMNANSFIAVKGKKGIPNEEMVYEITGIMRARHCLKPGTRDDFSVNEPS
                     MLTQGLAGVFSIVTIAGWVIGGFSLLVGGFGIANIMFVSVRERIGVIGIQKSLGAKNY
                     FILVQFLVEAIFLSLFGGIFGLILVWLLTLIASAALNFEIILTTGNVALGLVVSGAIG
                     LIAGMIPAYSASKLDPVEAIRHN"
     gene            complement(3970..5007)
                     /locus_tag="PKY63_10435"
     CDS             complement(3970..5007)
                     /locus_tag="PKY63_10435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF01074.1"
                     /translation="MDSDEINNNLDSSELPDEERELFEHFRIVVDRGQAQLRIDKYLS
                     DRIGNKVSRNRIQSAARAECILVNGKAVKPNYKIKPADEIVVVLPQPPHELGILPEEI
                     PLTFVYEDQQLAVVEKEAGMVVHPGFNNFDHTLVNALLYHFSKGKSDQHFPLLVHRID
                     KDTSGLLLVAKEEFAQTYLAKQFFDHTIDRLYRALVWGDFDEDSGTISGYLARSTRDR
                     RMMAVYDNEEEGKWSVTHYKVIERFGFATLVECRLETGRTHQIRAHMKHIGHPLFADE
                     MYGGKTLVKGNPTTKFKQFIENCFDIMPRQALHAAVLGFIHPGNGKHILFESPLPMDF
                     AALLEKLRKKV"
     gene            complement(4989..6857)
                     /locus_tag="PKY63_10440"
     CDS             complement(4989..6857)
                     /locus_tag="PKY63_10440"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01075.1"
                     /translation="MRNIFAVLSFLMFFSALSAQEILIPSGMNQSIIQQEPLAGKNNR
                     AILTMPFLEDFSATFVYPDPNRFSDSTAFINTGNAFAPISMGVATLDGLDAYGYLYNH
                     GSSFAFAADTLTSYDIRLDSVFTGTPHATSIADSLYFSFFYQPQGLGEKPDVEDSLLL
                     EFYAANQNAWIQVWAAPGSSLNDFIATNGSPWKCVMLPIIDTAFMTDGFRFRFRNYAS
                     YADLSFPTWASNADFWNIDYIILDSERDAADSVPTDLAFGERHETLLKKYFSMPWNHF
                     LANSAAEMSPEINAPYANLSESLLNVTERLTVDDLSGTTGDYDSGLSASNLNPLTDTA
                     FYRSPIPYTFSSSVSKNAEFEVNLSVNTATIGDPVASNDTLAFYQRFYNYFAPDDGSC
                     EAGYGLTVNSARAAYMFTLNMPDTLQSVQMFFNRVVNDANQIYFYLTIWDDNGGVPGN
                     IIYQQMGVRPEFEHGFNAFHTYVLDNPLPVSGRIYVGWQQTSSEVLNLGYDLNNDRSG
                     YFFYNVDGNWYNTLYAGTPMIRIIVGDSPYQHVGMSDTETLTSVFPNPCNDCAAIQFN
                     DNQSKTVSVIDLNGRVVKRIRCDNTMSTEDIAPGYYTLMIEKNAEHPVFVKWILTK"
     gene            complement(6871..7485)
                     /locus_tag="PKY63_10445"
     CDS             complement(6871..7485)
                     /locus_tag="PKY63_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPF01076.1"
                     /translation="MPDFSGMTMEEVKEMRESRHYEFLIVDSVYDNTKTPGSVVSQLP
                     LPGSHVKKGRSVYCTIIAFTPEKTKLPNLVDFTYRQAEALLQTHGLKVGKVERVPDVG
                     QTVIKAKYKGKVISWGTELNKGEKIDLVIGKGKGGGEVYVPKLVGKSREKAHQLIHEA
                     GLNVGSEIFADPDDTLRVRVIKQSPKYEKDKEVSVGESVDLWYE"
     gene            7501..7719
                     /locus_tag="PKY63_10450"
     CDS             7501..7719
                     /locus_tag="PKY63_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01077.1"
                     /translation="MKGVKPEKKKSYPYEDKTRQHNQGEMFPKMEAFDETENFHTAIK
                     LILLRFIVKTSEFCAKIQFLLVRRAWGE"
     gene            8223..8669
                     /gene="tnpA"
                     /locus_tag="PKY63_10455"
     CDS             8223..8669
                     /gene="tnpA"
                     /locus_tag="PKY63_10455"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPF01078.1"
                     /translation="MDRFSQILYQVVFGPRRREPIMTTPNREIFHKYLGTVLRNKKCF
                     VYAINGTTDHVHLLFSLHPSVSLSDLIKDVKLSTSSFIRTKKLFPMFDGWQEGYGAFT
                     YTYSAKDNLIRYVSNQQEHHKKISFYEELKNLLIENEINFNERYLI"
     gene            complement(9691..>10471)
                     /locus_tag="PKY63_10460"
     CDS             complement(9691..>10471)
                     /locus_tag="PKY63_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPF01079.1"
                     /translation="IALLQPDIHWKSPEMNRAMFDELFSLQAAKADVFVLPEMFTTGF
                     VSDPASIAEHPDGPTFQWMKNSAARFGCAIAGSIVISENNNYYNRFFWVNPDGSHYSY
                     DKRHLFSLGNENLAYSAGKEQVIFEYKGWKIKPLICYDLRFPVWARNSYNQGEFGYDI
                     LLLTANWPASRKLHWRQLILARAIENQCYAVGVNRRGKDGNGWQYAGGSTLATPDGKW
                     LPEREDGDSDVIFVELSKTELTQYRKAFPFAQDWDGFGLTP"
BASE COUNT         3170 a         2317 c         2128 g         2856 t
ORIGIN      
        1 atatgtgact cctccggagt cgtccaaaaa ataattgcgc cgatgaatca atcgttttgt
       61 tgttatttca gttcagcaag agcggcgcga ttttctcgaa attcggagcc cgaagggaaa
      121 tagcagacat agctgcaatt gtcccgtagg ggcaaaatat tggtagcacg aaaaagaacg
      181 ccagcccgtg ccgtcaggta cggaatggta ttactcaatg aaacctcagg agtcttccaa
      241 aaatgattgg gctgaggcat caatcgtttt gatattatct acccgaatgc agtcgggtgc
      301 gtgaataacc ggtaaatcag tgtcaaccag attgcgtaca tctgtcaggt ttagcacaat
      361 gaaatgtact aacagctttt tgaatctttt tgtgatattg cggaagttca actgattttt
      421 gtattttcga taagaaatca caaaaaatat ggctgcgcca gacgacaatt ttgttaagca
      481 aaacaattac aactttgcac ttgaagagga gtttgattat cttgacgaac tcagccggga
      541 acttgaagag caacggaaaa cagcggaaaa aaccacgcca atgactataa aagaaaacgg
      601 cttcgaagaa aatgaatcat cgaagccgtt aaaagaataa taagcactct aacaattcag
      661 atatttctgt accaaatcgc cgattgactt aattctctga tcatcgggca aagcgaaaag
      721 tcgttcactc agacctgaat catcgtagct attgagtttt tttatcaaag aatcgagcac
      781 ccttggcggg aaaacgccag attcgagata aaaatctttt tgcttttcaa gggctgttgc
      841 tgaatcagcg cagcaaatgg gaagctgttt cagtttttcg gcaacagctt tatgttcttc
      901 atggaaaata ttcacattga cataggtatc atttgctttc tgcaaagcat ccttgtcggt
      961 gaggccatga gtagcagctg ttaccagtcc ggccagcaaa agataaatat cggccgatcc
     1021 atccgggcag cgaaactcga aagtttgttt ttcgcgggta tccaatgtag cttccttgtt
     1081 tcctggattt gcgatggcgg tcattttcga ggcagcacca gcccatccca gcggaacacg
     1141 tacaagcacc gaacgattgc ggtcgcccca gcaaatattg gtaggagctt cctggtgagg
     1201 caccaggcgc aaatagcttg ttggaacagt attaccaaag gcagtaagtg atgcagacaa
     1261 atccataatt ccggcaatgg ctttgcgtgc cacatcggta aggccatttt catcgctggt
     1321 aacacttaca ccatctttga gcaatttcat gtgaatatgc aaaccgcttc cggccttgcc
     1381 aaccgtaatt tttggtgcaa aactcagcga aacgccatac ttataagcaa gactgcgaag
     1441 aatccatttc cccagaatga gctgttcggc tgcttcttca atatcagtcg gcagaaattc
     1501 aatttcgttt tgctcataat tataaaattc atcactgaaa tttccgactt cggaatgtcc
     1561 atatttgatt ttaccaccca tttgagcaat ataaagcatg gcttccttgc gcagatgctc
     1621 ccatttggta aatggcaccg actcgtgata acctttctga tcgaccgctt cgaacaaatc
     1681 ttcgcgttcg ctgataacat aatactcaag ctcgcccatg gcatgcatgg aataaccggt
     1741 tttatttacc agattttcgt gggcgcgcag caaaatgttt tcggggctca tttccatcgg
     1801 atttccgtct ttgtcgaaat aacgacaaaa tattccgagt gtcggaattt cactgaaagg
     1861 atttatgaat gcagttctga atttcggtac aacataaaga tcgctggagc cgctttccat
     1921 aaaaggaaac aaagaactgc catcgacacg ttcgccggaa agcagcaatt ggcgcagctg
     1981 ttcttcgcca gatacaacaa aattcagagt ctttattctg ccatctcctc ctgtgtaatg
     2041 aaaattgagc atctgaatct tagattcagc cacaaaagcg atgatgtcgt ccactgaaaa
     2101 ttcctgggga gttttcttca ggaattttac cagtggattt gggcataact gaaaactttt
     2161 ttccatatct attgattttt atgaatgata gtcaggacaa atatagaagt tattccgtta
     2221 caggcaagaa attcctgcca tttttcatta ttctgagcaa aaatgctgga ataaagatgc
     2281 tgatgttttg tttgacaaaa tcatcggatt tgctacacta aaaaactcag atacaaatcc
     2341 aacactctaa aaaataaaat caattatgac gtatggcttc caccggatcc agctttgatg
     2401 cactgtatgc aggtatcata cccgcgatga gtccgatggc acccgaaaca acaagtccaa
     2461 gtgcaacatt gcctgtcgtt aatattattt caaaattaag agctgcagat gctatcaaag
     2521 taagcagcca aaccagaatc agcccgaaaa taccaccgaa caaactcagg aaaatagctt
     2581 ccaccaaaaa ctgcacaaga ataaaataat tttttgcgcc cagcgatttc tgaatgccaa
     2641 tcactccaat tctttcacga accgaaacaa acataatatt ggcaatacca aacccaccga
     2701 caagcaagga aaatccgcca atgacccacc cggcaatcgt aacaatacta aatacacctg
     2761 ccaatccctg agtgagcata cttggctcat tcactgaaaa atcgtcacgg gttccgggct
     2821 tcagacaatg cctggcccgc atgatacccg tgatttcata caccatttct tcattgggaa
     2881 tacctttttt gcctttcaca gcaataaagg aattcgcatt catttcatcg tcaacatcaa
     2941 tcacctggcg gataaaatta aaaggcacca tgacaacttc atcattactg ttaccgatat
     3001 tattggttcc ttctttttta agcaccccaa taactttcac ctttttattg agaaacttga
     3061 catattgtcc aatcggatca ttcactccaa agagattttc ggctatggta gcacccagca
     3121 aacacacagc aactccattg ttgaactcac tcgggctgaa ataacgtccg cgggcgatat
     3181 caaagacaaa gaccttgttg tatgcttcag atattccgcc aattccaaca gattcgaatt
     3241 tgctgtcacc aaattcaaca ttgctcatag ccgatgacat aatagcagcg ctttcaatgg
     3301 ttgaagcgtt tttctcaaga gctttcaaat cttcaataac tggctgcggt cgtttggcat
     3361 attcccacca ttcaaaatcc ttactgaaac tccatggcca tttctgcaca aaaaccacat
     3421 tggttcccat cgactcaata ctggataaaa tctgattttt cattgaatca acgaccgtga
     3481 aaacaataat taccgaaaaa atcccgatgg taataccgag tactgtaagt attgtgcgaa
     3541 gccggttcac aagaatggcc gaaatggcaa acaaaaaact ctctttgata agccgcagga
     3601 ataacatgac acaaatatat aaaataaagc atgacacaca acacgcaaaa ctcaaagctc
     3661 aaagcagatg cacaattctc aattcattgt tatgacgaca aaataaaagt ttatttcaag
     3721 gtctttcgaa aattgaaaat ttcaagttct attctcataa atctatcttg ctgctgtatt
     3781 gttttcattt ttcaaatgcg ttggtatatg aggaaattct gcaaaatcct tgcatttaat
     3841 accacagtga tttataaatc ggttcaaaat ttttgagaga ctccggagga gtcatccaat
     3901 gaaatcaatg tgcgttttaa tccgcagatg atttttcacc taattttatt cttgttaagc
     3961 attgtgccgt cagactttct tgcgaagctt ttcaagtagg gctgcaaagt ccattgggag
     4021 cggactttca aacaatatgt gtttcccgtt tcccggatga ataaaaccaa gcacagctgc
     4081 atgcaatgcc tgccgcggca taatatcaaa acaattttca ataaactgtt tgaatttggt
     4141 agttggattc cctttcacca gggttttccc accatacatt tcatcggcaa aaagcggatg
     4201 accaatatgt ttcatatgag cacggatctg atgcgtgcgg ccagtttcga ggcgacattc
     4261 aacaagagtt gcaaaaccaa atcgttcaat aaccttgtaa tgagttacag accattttcc
     4321 ttcttcttcg ttgtcgtaaa cggccatcat gcgccggtcg cgtgtacttc gggccagata
     4381 accgcttatg gttccactgt cttcatcaaa atctccccag accagtgcgc gatacagacg
     4441 atcgatggta tgatcaaaaa actgctttgc cagatatgtt tgcgcaaatt cttctttggc
     4501 caccaatagc aacccgcttg tatccttatc gatacgatgt accaacaacg gaaaatgctg
     4561 atctgatttg cctttgctga aatgatacaa caacgcattt acgagtgtat gatcaaaatt
     4621 gttgaaaccc ggatgaacaa ccattcctgc ttccttttca acaacagcca gctgttgatc
     4681 ttcataaaca aatgtcagcg gaatttcttc aggcaatatt cccagttcat gcggtggttg
     4741 cggcaaaacg acaacaattt catcagcggg tttaatcttg taatttggtt ttaccgcttt
     4801 gccattcaca aggatgcatt cagcacgggc tgcactctga atccgattgc ggcttacctt
     4861 gtttccaatt ctgtcagaca aatatttatc aatccgcagc tgagcctgac cacgatcaac
     4921 aacaatccgg aaatgctcaa acaactccct ctcttcatcg ggcagttcag atgaatccag
     4981 gttgttattt atttcgtcag aatccattta acaaataccg gatgttccgc gtttttttca
     5041 atcatcagag tataatatcc gggcgcaata tcttcggtgc tcattgtatt gtcgcacctg
     5101 atgcgcttta caacacgtcc attcaagtca atgaccgaaa ctgttttgga ctgattgtca
     5161 ttgaattgta tcgcagcaca gtcattacat ggattgggaa aaacagaagt taatgtttcc
     5221 gtatcgctca ttccaacatg ctgataaggc gaatctccaa caataattct gatcatcgga
     5281 gttcctgcat acaatgtgtt ataccaattt ccatcgacat tatagaagaa atatccgctg
     5341 cggtcattat taagatcata gccaagattg agcacctcgc tgcttgtctg ttgccatccg
     5401 acatagatgc gaccactgac cggaagcgga ttatcaagca cgtaagtatg aaaagcatta
     5461 aacccatgct caaattcagg acgcactccc atttgctgat aaataatatt accgggcaca
     5521 cctccattat catcccaaat ggtcagataa aaataaatct gattggcatc gttcacaacg
     5581 cgattaaaaa acatttgaac agactgcaaa gtatcaggca tattaagtgt aaacatataa
     5641 gcagcccttg ccgagttcac cgtcaggcca taaccagctt cgcaggaacc atcatcaggc
     5701 gcaaaataat tataaaaacg ctgataaaaa gccaatgtat cgttcgatgc aaccggatcg
     5761 ccaatggtag ccgtattgac agacagattt acttcaaatt cggcattttt cgaaacagaa
     5821 gaactgaaag tgtaaggaat cggactgcga taaaaagcag tatcggtcaa agggttcaga
     5881 ttggaagcgc tgaggcctga atcgtaatca cccgttgttc cggacaaatc atcaacagtc
     5941 agacgttctg ttacattgag cagactttca gataaattgg cataaggagc attaatttcc
     6001 ggcgacattt ctgcagcgct attggcaagg aaatgattcc agggcatgga aaaatacttt
     6061 ttcagcaatg tttcatgacg ctcgccgaaa gcaagatcgg tcggaacaga atcagcagca
     6121 tcgcgctcac tgtcgagaat tatatagtct atattccaaa aatcggcatt agaagcccat
     6181 gtcgggaatg aaagatcggc atagcttgca taatttctga atcggaatct gaaaccatcc
     6241 gtcatgaatg cagtatcgat aatcggcagc ataacacatt tccacggact tccgttggtt
     6301 gcaataaaat cattgagcga agagccagga gccgcccaga cctgaatcca ggcattctga
     6361 ttggccgcat aaaattcaag cagcagcgaa tcttccacat ccggtttttc acccaaaccc
     6421 tgcggttgat aaaagaaact gaaatacaat gaatcagcaa tgcttgttgc atgaggtgtt
     6481 ccggtaaaga cagaatcaag gcggatatcg tatgaagtca gcgtatcagc agcaaaagca
     6541 aagctggatc catgattata gagataacca taagcatcga gcccatcgag agtggctaca
     6601 cccattgaaa ttggagcaaa agcattgcca gtatttataa aagcagtact gtctgaaaac
     6661 ctattgggat caggataaac aaaagtagca gaaaaatctt ccaggaaagg catagtcaaa
     6721 atagcgcgat tattcttgcc tgcaagaggt tcctgctgaa tgatggattg attcattcct
     6781 gatgggatca gaatttcctg agcactaagg gcagaaaaaa acatcagaaa agaaagaacc
     6841 gcaaaaatat ttcgcataag attaccgatt ttattcatac cagagatcga cagattcacc
     6901 aacacttact tctttatcct tctcgtactt cggactttgc ttaatgaccc ttactcgcag
     6961 cgtatcatca ggatctgcaa agatttcaga accgacattg agaccagctt cgtgaatgag
     7021 ctgatgagct ttttcgcgtg atttcccgac aagtttcggc acataaactt caccaccacc
     7081 tttgccttta ccaataacca gatcgatttt ttcgccctta ttcaattcag ttccccaaga
     7141 gataacctta cccttgtatt tggctttaat cacagtctga ccaacatcag gcacacgttc
     7201 taccttaccg acctttaatc catgagtctg taaaagggct tccgcctgac ggtaagtaaa
     7261 atcgaccaga ttgggtaatt tggttttttc aggggtaaaa gcaatgatag tacaataaac
     7321 cgatcggcct ttctttacat gcgaacctgg caatgggagc tgactcacaa ccgatcccgg
     7381 cgtttttgtg ttgtcataaa ccgaatcaac aatcaaaaat tcataatgtc tgctttcacg
     7441 catttcttta acttcttcca tagtcattcc ggaaaaatca ggcacttcga ttgattggcc
     7501 atgaaaggtg taaagccgga gaaaaaaaag agttatccat atgaggataa aacccgccag
     7561 cataatcagg gcgaaatgtt tccaaaaatg gaagcttttg atgaaactga aaattttcat
     7621 acagcgataa aactgatttt actgagattt attgtgaaaa caagtgagtt ttgtgcaaaa
     7681 atacaatttt tattggtaag gagggcgtgg ggagaatgat cccgtgctgt gaaaacctgg
     7741 cataaattga taataaattt ccggtggatt cgaccccctc cccgggtcgg ctgttttggg
     7801 gggaccttta acccggcctg cggccgggct atttttattt gacctgcgct gcagatcggg
     7861 atggttgtat caatcaatgg ttaaaggttt atgtgccgca ggtcggagtg gtaatacgca
     7921 ttttggtcat ggagtcaagt gccataggtc acggtgtgat tacacgcatc aaaggccatt
     7981 gatatatgca cccaataagg acttccagag gcttgcccga ccaacagagg cgggaagtcg
     8041 aataaaaata gcgtccggca caagccgaat gtgaatagat aaataacaaa cggccctgca
     8101 ggggccgaat acaaataaaa acgacaacaa ataattacaa acgtttatat ccgacaataa
     8161 acgtttaata accgaataaa gcctttcgcg tattctacat ttgcatatca aatcaaaatc
     8221 atatggacag attttctcaa attctctacc aggttgtatt tgggcccaga cgcagagaac
     8281 caattatgac aactccaaac cgggaaattt ttcataaata tctcggaacc gttttgcgaa
     8341 ataaaaaatg ttttgtgtat gcaatcaacg gcacaacaga tcatgtacac cttttgtttt
     8401 cgttgcatcc atcggtttct ttatcggatc tgattaagga cgtgaagttg tcaacatcat
     8461 catttatccg cacaaaaaaa ttatttccaa tgtttgatgg atggcaggaa gggtatggtg
     8521 catttacata tacatattct gcaaaggaca atttaatcag atatgtttca aatcaacagg
     8581 agcatcataa gaaaatcagc ttttatgagg agttgaaaaa tctcctgatt gaaaatgaaa
     8641 ttaattttaa tgagaggtat ttgatttaat tggatttcaa tatacctgga ttcgaccccg
     8701 ccggggtcgg tatttattct cttttatacc cggccttttc acgtaaaacg tgacacagct
     8761 ggccgcgcta tttttgttcg acctgcgctg caggtcgggg agtcaatatg catctattat
     8821 aaaaattcca gctcctcatg tcgggattga ggccttacac aaacaactat aaattatatt
     8881 ttggcaaaac atggttgtaa acaaaatgcc ggatgaacta ttttaatcgc tgaaggccgg
     8941 ggttatgatt caccagcata gttaatgatt tcatgcatgc aatccgattg gttttttgtg
     9001 gacaggttta aaatcaaaca gatgctcacc ctgcacccaa taaggacttc cagaggaagt
     9061 cgaataataa tagcgtccgg cgcaagccgg atgtgaatag ataaataaca aacggccctg
     9121 caggggccga atacaacgaa atatgggtct cgaaaattat gaggggcaaa tcaatctgga
     9181 ttcgacccct gcagggtcgt ttccgctgtt atctcttacc cggacttcgt ccgggctatt
     9241 tttatttgac ctgcgctgca ggtcgggggg atgaattatg tgaatgtaat aataacattc
     9301 acacattacg aaacctgctc gcaatgcgga cttccggagg cttgcccgac caacagaggc
     9361 gggatgtcga ataaaaataa cgtccgccgg ctggcggatg tgaatagata aataacaaac
     9421 ggccctgcag gggccgaata caacgagatg tgtttttgtt gttgaaatta attcgtttta
     9481 attgcgtttg attcgacctc gcagaggtcg ggttggctgt tatctcttac ccggacttcg
     9541 tccgggctat ttttatttga cctgcgctgc aggtcgggga tacaaataga ttctttgatc
     9601 tgaattcaag cgctgaagat cggaaaggtg aaatatttga tcttgcaaaa aattttttgt
     9661 tcacctccga aatatcgagt tattgcggat ttacggagtc aaaccaaacc catcccaatc
     9721 ctgcgcaaag ggaaatgctt ttcggtattg agtgagctca gttttactaa gttcaacaaa
     9781 aattacatcg ctgtctccat cttcccgctc cgggagccat tttccatcgg gggtggcgag
     9841 cgtggaaccg ccggcatact gccagccatt gccatctttt ccgcggcggt tgactccaac
     9901 tgcataacat tgattttcga tggcgcgggc cagtatcagc tgccgccagt gcagtttgcg
     9961 cgaggccggc caattggcgg tcagcaataa aatgtcgtag ccaaattcgc cctgattgta
    10021 cgaattgcgc gcccagacag ggaagcgcag atcgtagcaa atcaatggtt ttattttcca
    10081 tcctttatat tcaaatatca cctgctcttt tcccgcgctg taagccagat tctcgttgcc
    10141 cagcgaaaac agatgtcgct tatcgtagga gtaatgcgat ccgtcggggt tgacccagaa
    10201 aaaacggttg taataattat tattttctga aataacaata ctacctgcaa tggcgcatcc
    10261 aaagcgggct gcagaatttt tcatccattg aaatgttgga ccatccggat gttctgcaat
    10321 cgatgccgga tcggacacaa agccggtggt gaacatttcg gggagcacga aaacatcggc
    10381 tttagcggct tgcaatgaaa ataattcatc aaacattgcc cggttcattt cggggctttt
    10441 ccaatgaatg tcgggctgca gcagggcgat t
//
LOCUS       DAOPKE010000094        10309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6810_length_10309_cov_12.771504, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000094 DAOPKE010000000
VERSION     DAOPKE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_10309_cov_12.771504"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..722)
                     /locus_tag="PKY63_10465"
     CDS             complement(12..722)
                     /locus_tag="PKY63_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF01080.1"
                     /translation="MGRAFEYRKARKFKRWGNMSRMFTRYGREITMAVKEGGPSPEYN
                     TKLRAIIQNAKSDNMPKDNIERAIKKATEKDTADYKEVSFEGYAPHGIAVYVHCATDN
                     NTRSVANIRSYFNKYNGSMGTNGSVSFMFDKKCVFIFKDTGQDIEELELELIDFGAEE
                     IFKNEDGIQVYGDFSSFGTLQKAIEEKGFEITKADYDYLPNVELKHLSPEEQADVDKL
                     IEKIEDDDDVVRVYTTMA"
     gene            864..1601
                     /locus_tag="PKY63_10470"
     CDS             864..1601
                     /locus_tag="PKY63_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01081.1"
                     /translation="MKIHKTLLVCCLCMLSGMLTHGQIQLESTLTTGQNQTKPEGFLN
                     MAICPAYELNQWTFGAGAELAVLNPQNTMLDAWKLDAGRKILIKKHEYKLYGGFYCRP
                     FSEYLRELNLVLTIKTEYGHFRGMIGNNSRIWRLSGKAIEQWPEADSAGTIMKKEYRN
                     LLYDFSVYLKKTDHPWNIGLGFSNLNGFFFNQETTPMLFSRYENKITESMQLFAECWF
                     QQAGSANLQTQYFGFFIKGGLVWKSGK"
     gene            1583..2431
                     /locus_tag="PKY63_10475"
     CDS             1583..2431
                     /locus_tag="PKY63_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF01082.1"
                     /translation="MEKWKIIIALISISLTSCFHDLDEAGAFHSKYNTNERFEMSQEW
                     NSTHPFQVILMPDSTYSVLYCADTHIGTYKEFSMVLDAAKNPSNAALVVAGDVTRGHT
                     ESYDTLETLMNTVDSVNWFLTTGNHDLYFGGWDEYFPRFGASTYYFIVSTPAGNDLFI
                     ILDSGSGSLGNLQIDWLSNILESFHPSVRHISIATHLNVFKDGLGLTSGLPMEEVNVL
                     LDLCTRYEVDYVISGHDHTRFETTLGVTDYIIMDQCHDEESNSSYLEVRYEKNHCSHY
                     FNAIAE"
     gene            2448..3689
                     /locus_tag="PKY63_10480"
     CDS             2448..3689
                     /locus_tag="PKY63_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HPF01083.1"
                     /translation="MQTAVIIFTITFLGIIFTRLPFVNIDRPSAAFFGAVAMVLFGIL
                     SPAEALESIDFNTIALLLGMMIIIAVMQLDGFFNWLASKTMGFAGNQRRLLTIIIFVT
                     GIASAFMVNDVVVLMFTPVVIAICRQSGLNPVPFLMGVIFSANIGSAMTITGNPQNML
                     IGISSGISYARFLLLIAPISILGMLVIIPVIRLMYKKDFQIKKTVISNPHTFGFKLRK
                     MRWSIPVFSMVILLFFIGKPLGLSIPVIALAGASLILLVGRIKPSHVIKKVDWVLLLF
                     FAGLFVVVKGFENSGALENLLSIFPLNNNAGGMLAVHGISFVASQIVSNVPFTVAFLP
                     FMHEANSEMLWIALASASTLAGNATIIGAVANIIVIESAAKHDVKIRFFEFLKPGIVV
                     TLLSLLISCIIIVLWMHAAAF"
     gene            3922..4329
                     /locus_tag="PKY63_10485"
     CDS             3922..4329
                     /locus_tag="PKY63_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01084.1"
                     /translation="MKTLTFLLIIALMASFNVSAAIDDTKDSTEVAGATGPVIVCTID
                     KTFNRSYATDMEMETVTCSCYEVFKAPEIKEIVSNPVYKTITVTGTEHGDQIYILNSK
                     GEILLHVEAEIDNPKIDISKLTAGTYTIGVQAY"
     gene            complement(4405..5274)
                     /locus_tag="PKY63_10490"
     CDS             complement(4405..5274)
                     /locus_tag="PKY63_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF01085.1"
                     /translation="MRFFLIYIFLVLCAENVHSQSVSEGVVIPNTSEYVYKSEINHVS
                     YRISVSLPAEYSDSVNRRFSVIYFLDGNVWLPLASSVLQSINKMESIEPIIMVGIGYE
                     VETFEYDVVQRTYDYTPTFDYSADSSMTFRYQRPIRSGGADTFLLVLEQEIIPFIDSL
                     FRTTENRTIAGHSLGGLFATYVMLEKPRLFSNYIIASPAVWWDDNSLLLQWKARKNMV
                     MPDGKIMLGIGENETKRMMNAAKQLNTLMRKQLKNRNNLRYENIPDSDHFSMIPDMIM
                     QSLLFVFPAEKQQ"
     gene            complement(5283..8222)
                     /locus_tag="PKY63_10495"
     CDS             complement(5283..8222)
                     /locus_tag="PKY63_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPF01086.1"
                     /translation="MRGILFSIAILAMLPLFAQQQVSIHQTQSEYYATHDYGDTVAFG
                     VSKCIKNTGCILNRRVFGWHPYWVGSSVSDNYQWNLLSDLCYFSYEFNASTGAPTNTH
                     SWATVDVVDSALANGTRVHLCVTMFNSTDHTTFFGSTTAQTALISNLVNAVDSRGAHG
                     INIDFEGVSSTHKAALTTFIQRIADSLHTRVPGAELSIAMPAVDWSPSEWDLPAIIPY
                     VDLFIFMGYDYYYKGSGTAGPTDPLYNFQTTYNFTLSKTITYYLNAGVPKTKLMAGLP
                     YYGFQWKTAALTVPSSADSTGEAVTYSTIRNNSSGYYSNANKGYDVNSQSPYWAFNSA
                     GQNYQCWVNDAQSMGRRFDLVNIRDIAGIGIWALAYDNGYSDFWDLIADKFSDCAVVP
                     CTDTIWDLGGPTRPHYAYEDFVYTIQPSGATGVSLSFTEFDLEADYDSLWIYDGTSTA
                     AALIGGYSGTTSPGLVTASSNALTLEFHSDGATQNAGYTAIWTCSTDNIAPTTAINAP
                     SGWQTDDFGVSFTDADNNALKNSFWQVLQFDGTDWKGNNKAGFINENYNTTVPADWIS
                     YLGCWTWDNGHLLQTDSALGNTNLAISLPQDSSHVWLYHWQLKFGAADATINRRIGAH
                     YFCSDMSQSNRENNYMAYFRLDNQRVQLYEYVSNTYNLMTDVAYPFVVDTWYDVKVMY
                     DPETGYNAAWVNDVLVSSWIDATPLKSGSGFSLRVGNSKAWYDDVKVYQSRSASEIIT
                     VGPDTLDMVQWQNPTPLTPSCRIKSIVLDEANLFSAPAGSDANIDYTKPIAPAWVGDV
                     YAPTQSDYDTLYYSGILQNNTACAVSDDPHSDVVNYFFSMGAFCGDTSVYNFVSSGDS
                     TANIGAFTNNGMYYTFVYTVNGAGLHSDTVCSDGIYVEQLTSVDEYGDKLKLFPNPVE
                     HNLNLIFPFEKNCEIRIMSSDGRLVMFAESGIADFVQLDVSALQLGMYLIEVMTEKPL
                     RSVFMKK"
     gene            8401..9507
                     /locus_tag="PKY63_10500"
     CDS             8401..9507
                     /locus_tag="PKY63_10500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF01087.1"
                     /translation="MKKILVLCPYPENCAPSQRLKYEQYFGEFRKAGYDISVSSFVNK
                     QFWKIIYLRNHWLAKAFHTLRGYLRRTADLFRMRRYDIVYVHLWVTPLGFPVYERLVK
                     YFARALVYDIDDMIFLGHSSEANHMLQNLKGKRKMSYLMKAADHVITSTPALEEFARK
                     YNHKVSDIPTTLQVDRFTAKTDYSLKNEIVLGHSGSHSTSKYLKSIEPAFEILLKSDL
                     KFKVLVTGDADFCFSNPAIPVEAKAWYAEDEASDLLRMDIGLYPLTDEPWVYGKRGGK
                     ALLYMAAGLPVIATAIGTNTETFSDGNTALLVKVNDFDDWAEKIFLLARNESLRQKLG
                     TAARKAVKTQFSVAANKDKYLAVLNEVIEKKNPS"
     gene            9660..9881
                     /locus_tag="PKY63_10505"
     CDS             9660..9881
                     /locus_tag="PKY63_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPF01088.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDFAIRSGAPMELIDNLREIE
                     DDEEVFESIEDLWPDYPTKDDFFFNEDEY"
     gene            10050..>10309
                     /locus_tag="PKY63_10510"
     CDS             10050..>10309
                     /locus_tag="PKY63_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPF01089.1"
                     /translation="MFRKEFKGLVYFSMKYVKDMDAARGIVQDAFIMLWDKRDSMDMA
                     RNPKSYLTTTIYTRSFNYLRDNKKFDKNIIEFEELVDSGSQSA"
BASE COUNT         3081 a         2229 c         2044 g         2955 t
ORIGIN      
        1 actttctacc gttaagccat ggtcgtataa acccgcacta cgtcgtcgtc gtcttcaatc
       61 ttttcaatga gcttgtcaac atcggcctgc tcttcaggag aaagatgctt cagctcaaca
      121 ttgggcagat agtcatagtc ggctttggta atttcaaaac ctttttcttc aatggctttc
      181 tgaagcgtgc caaaactgct gaaatcgcca tacacctgaa tgccgtcttc gtttttgaag
      241 atttcttcag caccaaaatc tatgagttcc agctccagtt cttcgatatc ctggccggta
      301 tctttgaaaa tgaaaacaca tttcttatcg aacataaaac ttacagagcc attggtgccc
      361 atacttccgt tgtatttgtt aaaataactg cggatattgg caacagagcg tgtgttattg
      421 tcggttgcac aatgtacata tacggcaata ccatgaggag catagccttc gaatgacact
      481 tctttgtaat cggcggtatc tttttcagtg gcttttttaa tggcgcgctc aatattatcc
      541 ttgggcatgt tgtcactctt ggcattctgg atgatggctc tgagcttggt gttatactcc
      601 gggcttggtc caccttcttt cacggccatc gtgatttcac gaccataacg ggtaaacatg
      661 cgggacatat tgccccatct tttgaatttt cttgctttac gatactcaaa tgcgcgtccc
      721 ataattctga ttttgagatg aaatgcaaaa ttaaaaaaaa gaatcccaaa atcagagaga
      781 atgcatgttt tttttcattt caaacaatct tcacgttttt ctcactgata aaagttacct
      841 atttttgtat tcaatagaaa tctatgaaaa tacataaaac actgcttgtc tgctgtcttt
      901 gtatgctttc aggcatgctt acacacggac agatacagct tgaatcgacc ctgacaacag
      961 gacagaacca gactaaacct gaaggttttc tgaatatggc catttgtccg gcgtatgagt
     1021 taaatcaatg gacatttggt gccggagccg aactggcagt actaaacccg caaaacacca
     1081 tgctggatgc ctggaaactc gatgcaggaa gaaaaattct cattaaaaaa cacgaataca
     1141 aactgtatgg cggcttttac tgccgaccct tttcggaata tttgcgcgaa ctaaatctgg
     1201 tgctgacaat taaaactgaa tatggacatt tcagaggaat gattggcaac aattcacgca
     1261 tctggcgctt aagcggaaag gcgatagaac agtggcccga agctgatagt gcaggaacaa
     1321 ttatgaaaaa agaatataga aatctgttat atgatttttc ggtttatcta aaaaaaacag
     1381 atcacccatg gaacatcgga cttggattca gcaatttaaa tggatttttt ttcaatcagg
     1441 aaaccacacc catgctgttc agccgctatg aaaataaaat taccgaatca atgcaattgt
     1501 ttgctgaatg ctggtttcag caagcgggca gcgcaaacct acaaacacaa tatttcggat
     1561 tttttataaa aggaggcctg gtatggaaaa gtggaaaata atcattgcgc tgatttcaat
     1621 ttcactcact tcgtgttttc acgatctgga tgaagctggt gcttttcact cgaaatacaa
     1681 taccaacgag cgatttgaaa tgtcgcagga atggaactcc acgcacccgt ttcaggtaat
     1741 actgatgccg gattccacat attcagtatt gtattgcgcc gacacgcata ttggaacgta
     1801 caaggaattc agcatggtac tcgatgctgc aaaaaaccct tcaaatgcgg ctttggtggt
     1861 tgccggtgat gttacacgcg gtcataccga aagttatgac actctcgaaa cccttatgaa
     1921 tactgttgat tccgtaaact ggtttctcac aaccgggaat catgatttgt attttggcgg
     1981 ctgggatgaa tactttcccc gatttggggc ttcaacctat tattttattg taagtactcc
     2041 tgccggaaat gatttattta tcattctcga cagtggcagc ggctcacttg gaaatctgca
     2101 aatagactgg ctttcaaaca ttctcgaatc cttccaccct tcggttcgtc acatcagcat
     2161 tgcaacacac ctcaatgttt tcaaagacgg actgggctta acatctgggt tgccaatgga
     2221 agaagtaaat gtgctgcttg atttatgtac gcgctacgaa gttgattatg ttatcagcgg
     2281 tcacgatcat acacgattcg aaaccacttt gggcgtcaca gattatatta ttatggatca
     2341 atgccacgat gaggaaagca actcttcgta tctggaagtg cgatatgaga aaaatcattg
     2401 cagccattat ttcaatgcca ttgcggaata aacaacgttt ctgattcatg caaacagctg
     2461 tcataatatt taccattact tttctgggaa ttattttcac ccgccttccg ttcgtaaata
     2521 tcgaccggcc atcagcggct tttttcggcg cagtcgccat ggtattattt ggaatactaa
     2581 gccctgccga agcgcttgaa tcaatcgact tcaataccat tgcacttttg ctgggcatga
     2641 tgattattat tgctgtcatg cagctggatg gattttttaa ctggttggct tcgaaaacaa
     2701 tgggcttcgc cggaaaccag cgcagactcc ttactatcat catttttgta accggaattg
     2761 catcggcatt tatggtaaac gatgtggtcg tgctaatgtt tacacctgtg gttatagcta
     2821 tttgcagaca atcaggccta aatccggttc cctttctgat gggtgttatt ttttccgcca
     2881 acatcggatc tgccatgacc attaccggca atccgcaaaa catgcttatt ggcatcagta
     2941 gcggcatttc atatgcacga tttctattgc taatagcgcc gatttccatt ttaggaatgc
     3001 tggtcatcat tcccgtaatc cggctgatgt acaaaaaaga ttttcaaata aaaaaaaccg
     3061 tcatcagcaa tccgcacact tttggattca aattacgcaa aatgcgttgg tctattccgg
     3121 ttttttcaat ggttatacta ttattcttca taggtaaacc gcttggatta tcaattcccg
     3181 tcattgcact ggcaggcgct tcgttgattt tactggtggg gcggattaaa cccagccatg
     3241 ttattaaaaa agtagattgg gttctcttgc tttttttcgc cggattgttt gtcgttgtaa
     3301 aaggatttga aaattcaggc gcactggaaa atctgttatc catttttcca ttgaataaca
     3361 atgctggagg aatgcttgct gttcacggaa ttagttttgt ggcatcacaa attgtcagta
     3421 atgtaccttt tactgtcgcc tttcttccat ttatgcatga agcaaacagt gaaatgcttt
     3481 ggattgcgtt ggcttcagct tcaacactgg caggtaatgc taccatcatt ggtgcagtag
     3541 ccaatataat agttattgaa tcggcggcaa aacatgatgt caaaatccgt ttttttgaat
     3601 ttctgaaacc cggaatagta gttactttgt taagtttact gatttcatgt attattattg
     3661 ttttatggat gcatgctgct gctttttgaa taaatgatta aaccaccttt attaaaccgc
     3721 tgaaagcata caaacagtca aaattattgc acaaaaaaac ttttttcaga caaaaggggg
     3781 ttttatgccg acttatcgga tggtgtaaca aaacaaactt ttataacgtc atattatcga
     3841 atttggtaga aaactgttgt tttcgctcat tcactaaagt aactttacta tacgaacagc
     3901 actactaaaa aactctgcgt catgaaaaca ctaacatttc tcctaattat cgctctgatg
     3961 gcttcattta atgtttctgc agctatcgac gataccaaag actcaaccga agttgccggg
     4021 gctacgggac cggtgattgt atgcacaatt gacaaaactt ttaacagatc ctacgctact
     4081 gacatggaaa tggaaacagt aacctgctct tgttacgaag tatttaaagc tcctgaaatc
     4141 aaagaaattg tttcaaaccc tgtttacaaa accattactg taaccggtac tgagcatggt
     4201 gatcagattt atatccttaa ttcaaaaggt gaaattttgc tgcacgttga agccgaaatc
     4261 gataatccca aaattgatat cagcaaactc acagcaggta cctacactat tggcgtacaa
     4321 gcttactaaa taatatattc cagaatcagg aataatcatg gttggtttat tttaccccga
     4381 cgccgtgagg tctgccgggg ttttttattg ttgtttttca gctggaaata caaacaacaa
     4441 ggattgcata atcatatcag gaatcattga aaaatgatcg ctgtccggga tgttttcata
     4501 acgaagatta tttctgtttt ttaattgttt cctcattaat gtattcaact gctttgcagc
     4561 attcatcatc cgcttcgttt cgttttctcc aattccaagc ataattttcc catccggcat
     4621 gaccatgttt tttcgtgctt tccattgaag caacaaacta ttgtcatccc accacactgc
     4681 ggggctggca atgatgtaat tgctgaaaag ccgcggtttt tcgagcatta catatgtagc
     4741 aaacaatcca cccagcgaat gtcctgcaat ggttctattt tcagtcgttc tgaaaagtga
     4801 atcaataaat ggtattattt cctgctccag caccagtaga aaagtatctg ctccgcccga
     4861 ccggatcggc ctttgatatc gaaaagtcat ggaagaatca gctgaataat caaacgtcgg
     4921 cgtgtagtca tacgtgcgct gcaccacatc gtattcaaat gtctccacct catatccaat
     4981 ccccaccatg atgattggct ctatggattc catcttattg atactttgca aaaccgagct
     5041 tgcaagaggc agccagacgt ttccgtcaag gaaataaata acagaaaaac ggcgatttac
     5101 actgtcggaa tattcagccg gcaacgaaac actgattcgg tacgaaacat gattaatctc
     5161 tgatttataa acatactccg atgtattcgg aataacaacc ccttctgaaa ccgactgaga
     5221 atgcacgttt tcagcacata aaacaagaaa gatataaatc agaaaaaacc tcatctgttg
     5281 aattattttt tcataaatac agagcgcaat ggtttttctg tcatcacttc aatcaggtac
     5341 atgcccagct gaagagcact aacatccagt tgaacaaaat cagcaattcc actttcagca
     5401 aacattacca aacgaccatc ggaactcata atcctgattt cacaattttt ttcaaaggga
     5461 aaaatcagat taagattgtg ttcaacagga ttcggaaaaa gcttcaattt atcaccatat
     5521 tcatcaactg aagtcagttg ttcaacatag attccatccg agcaaactgt gtcggaatgc
     5581 agtccggcac cattcaccgt ataaacaaaa gtataataca ttccattgtt tgtaaacgct
     5641 ccaatgttgg cggtgctgtc gcccgaagaa acaaaattgt aaacactggt atcaccgcaa
     5701 aatgcgccca tgctgaaaaa ataattcacg acatcgctgt gcggatcatc cgaaacagca
     5761 caagcagtat tattctgaag aattcctgaa taatacaatg tatcataatc ggattgtgtc
     5821 ggagcatata catcaccaac ccaggccggc gcaattggtt tagtataatc aatgttggca
     5881 tctgaacctg cgggtgcaga aaacaaattt gcttcatcaa gcactatgga ttttattctg
     5941 caagaaggag tcagcggagt tggattctgc cattggacca tatcaagtgt atccgggcca
     6001 actgttataa tctcagatgc agagcggctt tgatatactt tcacatcatc gtaccatgct
     6061 ttgctgtttc cgacacgaag cgaaaagcca ctccccgact tcaatggagt agcatcgatc
     6121 catgacgaga caagtacatc attcacccag gccgcattgt atcccgtttc cggatcatac
     6181 ataactttca catcatacca ggtgtcaacc acaaaaggat aagcaacatc tgtcatcagg
     6241 ttgtaagtat tgctgacata ctcatacaac tgaacccttt gattatcaag ccggaaatag
     6301 gccatgtaat tgttttcgcg gttgctctgc gacatatcgc tgcagaaata atgcgcaccg
     6361 atccggcgat tgattgtggc atcggcggct ccaaatttta attgccaatg atacagccac
     6421 acatgactac tgtcctgcgg aagcgaaatt gccagattgg tatttcccaa tgctgaatct
     6481 gtctgcaaca aatgtccatt atcccaggtc caacaaccaa gataagaaat ccagtctgct
     6541 gggaccgttg tattgtaatt ttcattgata aagccggcct tattgtttcc tttccaatct
     6601 gtgccatcaa attgcaacac ctgccagaaa gaattcttca acgcattgtt atctgcatcc
     6661 gtaaatgaaa caccaaaatc atcggtttgc caacccgaag gcgcattgat ggctgttgtc
     6721 ggagcaatat tgtccgtcga acaagtccag atggcagtgt aaccagcatt ctgcgtagca
     6781 ccatcgctgt gaaactctaa tgtcagtgca ttgctgctcg ccgtaaccag tccgggtgaa
     6841 gttgttccgc tgtatccacc aataagtgca gcagcagtag atgtaccgtc ataaatccat
     6901 aaagaatcat agtccgcttc caaatcaaat tcggtaaaac taagactaac accggttgcc
     6961 cctgacggct gaatggtata aacaaaatcc tcataagcat aatgtgggcg cgttggtcct
     7021 cccagatccc agatggtatc cgtgcaaggg accactgcac aatcggaaaa cttatccgca
     7081 atcaaatccc agaaatcgga ataaccatta tcatacgcaa gagcccagat gccaattcca
     7141 gcaatatccc gaatattaac caaatcaaaa cgtcttccca tcgactgcgc atcattcacc
     7201 cagcactgat aattttgtcc tgccgaatta aaagcccaat aaggagattg actgttcaca
     7261 tcatagccct tgtttgcatt tgaataataa ccagaggaat tatttctgat agtagaataa
     7321 gtcactgctt cgccagtaga atctgcagag gaaggaaccg tcaaagcagc cgttttccac
     7381 tggaagccat aataaggcaa gccagccatc agctttgttt ttgggacacc ggcattcaaa
     7441 taataggtga ttgttttaga aagcgtgaaa ttatatgtag tctgaaaatt atagagagga
     7501 tccgtcgggc ccgctgtacc actacccttg taataataat catatcccat aaaaataaat
     7561 aagtcaacat aaggaataat cgccggaaga tcccattccg aaggactcca atcgaccgca
     7621 ggcattgcaa tagacaattc tgcacccgga acacgcgtgt gcagcgaatc cgcaatacgc
     7681 tgaataaaag ttgtcagagc ggctttatgc gtactcgaca ccccttcaaa gtcgatattg
     7741 ataccatgtg caccccgcga atcaacagca tttacaagat ttgatattaa ggcagtctgc
     7801 gctgttgtgc taccaaaaaa agttgtatga tccgtagaat taaacatagt tacacacaaa
     7861 tgcacacgcg ttccgtttgc caaggcagaa tccactacgt caaccgttgc ccagctgtgc
     7921 gtatttgttg gcgcacccgt cgaagcatta aactcgtacg aaaaataaca caaatccgat
     7981 aacaaattcc attgataatt atccgaaaca gaactaccca cccaatacgg atgccagcca
     8041 aaaacgcgcc tgtttaaaat acaacccgta tttttgatac actttgaaac accaaatgca
     8101 accgtatcac cataatcatg agttgcataa tactcagatt gagtctgatg aatagatacc
     8161 tgttgctgcg caaaaagcgg caacatagcc aaaatcgcaa tagaaaataa aatccccctc
     8221 atatgaattg ttttttcaaa tgtaataaat ctccgaaaat ataaaccact gttttggtcg
     8281 tttttaccga cgaacgaaga ggcagaaaaa aaccggtggg gatgtagtca ttaaataaag
     8341 gttgcgcgaa gtttataaac ttcgcgcaac tttattaatt ttgtttgtca atacaatttt
     8401 gtgaagaaga ttctggtttt atgtccttac cccgaaaact gtgctccaag ccagcgtctg
     8461 aaatatgaac agtatttcgg cgagtttcgt aaagcaggtt acgacatttc ggtatcttct
     8521 tttgtaaaca aacagttctg gaaaatcatt tatctacgca atcattggtt ggcaaaagct
     8581 tttcatacac tgcgagggta cctcagacgc acggctgatc tcttccggat gcgtcgctac
     8641 gacattgttt atgtacatct ttgggttact ccactgggct tcccggttta tgaacggctt
     8701 gtcaaatact tcgctcgtgc tttggtgtat gatattgacg atatgatttt cctgggccac
     8761 tccagcgagg ccaatcatat gttgcaaaat ctcaagggta aaagaaaaat gtcgtatctg
     8821 atgaaggcag ctgatcacgt aataacaagc acgccggcac ttgaagaatt tgccagaaaa
     8881 tacaaccaca aagtttctga tattcctacc acattgcagg tcgatcgttt caccgcaaag
     8941 actgattatt cactgaaaaa tgaaattgtt ctgggtcaca gtggcagcca cagtacttca
     9001 aaatatctga aatcgattga acctgctttt gaaatattac ttaagtccga tttgaaattc
     9061 aaagtgcttg ttacgggaga tgctgacttt tgtttttcaa atccggccat tccggtcgaa
     9121 gccaaggcct ggtatgctga agatgaggcg agtgatttgc tacggatgga cattggcctg
     9181 tatcctctca ccgatgagcc ctgggtatat ggaaagcggg gcggaaaggc gcttttgtac
     9241 atggcggccg gattaccagt gattgcaacg gccatcggaa ccaacacgga aactttcagc
     9301 gacggcaaca cggccttgct ggtaaaggtc aatgatttcg acgattgggc ggaaaaaatc
     9361 tttttgctgg cccgcaacga atctttaagg caaaaacttg gaactgcggc cagaaaagcc
     9421 gtgaaaaccc aattctcggt tgccgccaac aaggataaat acctggcggt tttaaatgag
     9481 gttattgaaa agaaaaatcc ttcctaaagg aagttttcaa caaataataa ttttcaatcg
     9541 ttttttcaga ataaattttt atatatttgc tgactttttt agactaagta tctaagaaag
     9601 tccgaaaact tgatttttgc gttgaattat ttaatttttg caacaaaagg gaggatatta
     9661 tgtattggac tttagaactt gcttccaaac tcgaggatgc tccatggcct gccaccaaag
     9721 aggagctgat tgatttcgct attcgttcgg gagcccctat ggaattgatt gataatctta
     9781 gagaaattga ggacgacgag gaagtttttg aaagcatcga ggatctttgg ccagattacc
     9841 cgacaaaaga cgattttttc ttcaacgaag atgaatacta attgatgagc cgctgtaaga
     9901 gcggcttttt ttatgttgat aatgacagaa gaaaagatat tatcgttcat attattgaga
     9961 ttatatatat ttgaagttta aaagtcgaca ctctggaagg atcatctgca aatatcgctc
    10021 aggcactaag caaatcagct tttgaagatt tgttccgcaa ggaattcaaa gggctggtgt
    10081 atttttcgat gaagtatgtg aaagatatgg atgccgcccg tggcattgtg caggatgctt
    10141 tcatcatgct gtgggacaaa cgagacagca tggatatggc ccgcaatccg aagtcgtatc
    10201 tcacaacaac catctacacg cgctcgttca attatctgcg cgacaacaaa aaattcgaca
    10261 agaacatcat tgaatttgag gagcttgtcg attccggctc acagagcgc
//
LOCUS       DAOPKE010000095        10271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_6844_length_10271_cov_12.391347, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000095 DAOPKE010000000
VERSION     DAOPKE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_10271_cov_12.391347"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKY63_10515"
     CDS             complement(<1..266)
                     /locus_tag="PKY63_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01090.1"
                     /translation="MKKFQFRINGKIYDVELINVEGAAPVTQTAAPVAAPVAAPAATP
                     TTPVAETKAEAAPAGGNGKVTGHVLSPLPGILRSYSVKPGDRVK"
     gene            complement(273..488)
                     /locus_tag="PKY63_10520"
     CDS             complement(273..488)
                     /locus_tag="PKY63_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01091.1"
                     /translation="MKENNYLMNVSAEDKSEITDEILAIISAAVETCLSEKEKRTVQI
                     IRGSHPLSPWCSKTFLHRQTPVIVNKK"
     gene            complement(581..1732)
                     /locus_tag="PKY63_10525"
     CDS             complement(581..1732)
                     /locus_tag="PKY63_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPF01092.1"
                     /translation="MSFFEFLLQRFEQFFQYTAFTNMTIGHVIMIVVGLIFIYLAIKK
                     EFEPLLLIPIGFGMIVGNIPFYDGLKIGIYESGSVLNYLYFGVIQGVYPPLIFLGIGA
                     MTDFSALISNPKLFLIGAAAQLGIFGAYAIALALGFSPQEAGAIAIIGGADGPTAIFL
                     SSKLAPDLMGAIAVSAYSYMALVPVIQPPVMRLLTTKKERVIRMKPPRAVSKREKIIF
                     PIAGILLTCFIVPSGLPLLGMLFFGNLLKESGVTKRLADTARGPLIDIVTILIGLTVG
                     ASTQATTFLTAKSIGIFALGAASFVIATTGGVLFVKFLNLFLKEGRKINPLIGNSGVS
                     AVPDSARVSQTVGLEYDKSNHLLMHAMGPNVAGVIGSAVSAGILLSFLF"
     gene            complement(1750..2190)
                     /locus_tag="PKY63_10530"
     CDS             complement(1750..2190)
                     /locus_tag="PKY63_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPF01093.1"
                     /translation="MKQFSFKINGTKYEVEIGEIEDAHVEVNVNGSTYDVEVDRNIQP
                     VKTPKLVSKQTVPSTDISQQTMKTAPSGSVAGGGVIKSPLPGVILEVHVREGDKIILG
                     QKLVTLEAMKMENVINSDKEGTVQSIKFNKGDNVMEGDILIHIG"
     gene            complement(2202..3098)
                     /locus_tag="PKY63_10535"
     CDS             complement(2202..3098)
                     /locus_tag="PKY63_10535"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPF01094.1"
                     /translation="MNKVFQIALLLCFFQIQVINAQNQTDIRINEVMTLNCNGPVDGF
                     GHHSPWIEIFNSSYGTINIAGMYLTDDIHQPKKYRIPADSRMYMASRSYLLFYCNGFS
                     QHGVFYTNFQLDSTSCIYIFASNAQTIIDSVSFNFTETDQILARDVDGDGKWVLSKAY
                     TPNQSNVLIEPVKNAELFVKYDPLGIGMAIIAMSVVLSALALLYLLYKLISKLFKGDL
                     KASVKKKKHVSAGQIQLQTQEESELSGEINAAIALALSLYNNQLHDREETVLTIQRVN
                     KTYSPWSSKLYNQRQMPERHRK"
     gene            complement(3111..4670)
                     /locus_tag="PKY63_10540"
     CDS             complement(3111..4670)
                     /locus_tag="PKY63_10540"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPF01095.1"
                     /translation="MSIEKKISELMGLREKARQGGGLKRIDSQHAKGKLTARERISML
                     LDEGSFEEFDMFVTHNCHDFGMEDERYLSDGVVTGYGTIDGRLVYVFSQDFTVFGGSL
                     SEAYAGKICKIMDQAMKVGAPVIGINDSGGARIQEGVKSLGGYADIFQRNIMASGVVP
                     QISAIFGPCAGGAVYSPALTDFILMSKENSYMFVTGPKVVKTVTSEVVTDQELGGANI
                     HATKSGVSHFVAENEEEGMQIIRKLLSYLPSNNMEDAPFSECEDPIDRLEDSLNYIIP
                     DNPNKPYDVKDIINGFVDNGEFLEVHKHFAPNIVVGFSRLGGMSVGVVANQPCYLAGV
                     LDIDASKKGARFVRFCDAFNIPIITLVDVPGFLPGTQQEYGGIIINGAKLLYAYGEAT
                     VPKITITLRKSYGGAHDVMGSKQLRADINYAWPSAEIAVMGARGAVEIIEGRKIKEIT
                     NDAERAKFIEEKETEYRNKFASPYEAARYGFIDDIIEPRNTRFRIIRALQSLATKKQN
                     LPAKKHSNLPL"
     gene            complement(4672..5085)
                     /gene="mce"
                     /locus_tag="PKY63_10545"
     CDS             complement(4672..5085)
                     /gene="mce"
                     /locus_tag="PKY63_10545"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPF01096.1"
                     /translation="MEISHIEHIGIAVKSLTESIKYYEEVLGLKCYAIEEVADQKVKT
                     AFFMVGQTKIELLESTDPEGPIGKFIENKGEGIHHLAFAVKDIEKALTEAEEKGIRLI
                     DKTPRKGAEGLTIGFLHPKSTIGVLTEFCENKNTK"
     gene            5414..6274
                     /locus_tag="PKY63_10550"
     CDS             5414..6274
                     /locus_tag="PKY63_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF01097.1"
                     /translation="MKRQIIEIDEEKCNGCGICASGCHEGALQIINGKAVLINDLLCD
                     GLGACIGGCPEGAINITEREAEPYDEDKVMEHLHKKGKDVVIAHLKHLKDHNEHVWLH
                     KAVEWMQKNQDRLDFDMIEVVYVVHNHHKQGDSCGCSGSAHREIEHDTKNESVFAEQS
                     STLEQWPVQMHLINPLSSVFHNADLLMAADCVAFSMGNFHSKWLKGKKLAIACPKLDS
                     NKESYVEKLTSMIDDAKVNTITVMMMEVPCCHSLIRLVQLALENASRKVPVKAVVVSA
                     SGEVLSEEWV"
     gene            complement(6459..7292)
                     /locus_tag="PKY63_10555"
     CDS             complement(6459..7292)
                     /locus_tag="PKY63_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPF01098.1"
                     /translation="MYSLHNHTTYCDGKASVQEMISAACSEKLDHFGLSGHAPVPFEN
                     KWSIVSDEKVHEYCAEIDAAKNTCTSTKIWTGLEADYIPAITKPFDYWRNNFSLDYLI
                     GSIHLVENKGQLWFIDGPAEGYDIGLKEIFGDDMRAAVKSFYRQTCEMIETQKPDILG
                     HCDKVLMHNRNRRINAEDPFHLNLLKETLLLAAEKNVIVEINTRGIYRKLHTDYYPGK
                     YIFGFLKENNVRVMLSADAHMADQITGEFEHLKTDLAAAGLLDTWLPLCVSDKPVVLY
                     E"
BASE COUNT         2892 a         2099 c         2221 g         3059 t
ORIGIN      
        1 tttttcacac gatcgccggg ttttacacta tagctgcgca gaataccggg cagtggtgaa
       61 aggacatggc ctgtaacttt tccgttgcca ccggcagggg cggcttcggc ttttgtttcg
      121 gctaccggag ttgtcggagt tgctgccgga gcggcaaccg gggctgcaac cggagctgcg
      181 gtttgtgtaa caggagcggc gccctcgaca ttaatgagct ccacatcgta aatttttcca
      241 ttgattctga actgaaactt tttcatcttg tattattttt tgttaacaat tacgggtgtt
      301 tgtcgatgca aaaatgtttt tgagcaccat ggggagagtg ggtgagaacc acggataatt
      361 tgaaccgtac gtttttcttt ttctgataag caagtttcaa cagctgctga tattatagcc
      421 agaatttcat cagttatctc cgatttgtct tctgcagaca cattcatcag atagttattt
      481 tctttcatat gagtatttta tcgatttcaa aagtacaaaa aaaaataaag aaaccgttgt
      541 cgaaaaaacg gtttctttat aaaatctata tgtgtgtaaa ttagaagagg aagcttaaca
      601 gaattccagc agaaacagca cttccaatga ccccggcaac attgggtccc atggcatgca
      661 ttaataaatg atttgattta tcatattcaa gcccaacagt ttgcgataca cgggcgctgt
      721 cgggcaccgc tgaaacgcct gaatttccaa taagaggatt aatttttcta ccttctttaa
      781 gaaacaaatt cagaaatttt acaaacagaa cgccacctgt ggttgcaata acaaaagagg
      841 ctgcaccgag tgcaaaaatg ccgatggatt ttgcagtaag gaaagttgtg gcttgagtag
      901 aagctccaac agtaagaccg atcagaatgg tcacaatgtc aatcagcgga cctcttgcag
      961 tatcggccag tcgtttggtt actccacttt cctttaggag gttgccaaag aaaagcatcc
     1021 ccaatagtgg aagtccagaa ggaacaataa agcaggtgag caaaattccg gcaatcggga
     1081 aaattatttt ctcgcgttta gaaacggcac gtggtggttt catccgaata acacgttctt
     1141 tttttgtcgt cagcaaacgc attaccggcg gttgtataac cggaacaagt gccatatagc
     1201 tgtacgccga cactgcgatg gcacccatca gatccggagc cagtttagaa ctaagaaaga
     1261 tggccgttgg tccgtcggca ccaccaataa tcgcaattgc accggcttcc tgtgggctga
     1321 atcctaatgc cagagcaatt gcataggcgc caaaaatccc caattgagct gcagccccaa
     1381 ttaaaaacag cttagggttt gaaatcagcg ccgaaaagtc tgtcatagcg ccaattccaa
     1441 gaaaaatcaa aggtggatat acaccctgaa tcaccccgaa atacagataa ttcaaaacac
     1501 taccggattc ataaataccg attttcaagc catcgtagaa aggaatattt ccgacaatca
     1561 ttccaaaccc gatagggata agtaataatg gctcaaattc ttttttaata gcgagataaa
     1621 taaagatgag cccgacaaca atcataataa catgaccaat cgtcatgttt gtaaaggctg
     1681 tatattgaaa gaactgttca aacctttgta aaagaaattc aaaaaaactc atgagtaccc
     1741 tgctttatgt tagccaatat gaattagaat gtcgccttcc atcacattat cacctttatt
     1801 gaattttatg gactgaacag ttccttcttt gtcagaattg atcacgtttt ccattttcat
     1861 tgcttcaagt gtaacgagtt tttgcccgag aataatttta tctccttcac gaacgtgaac
     1921 ctcaaggatg acgcctggaa gtggtgattt aataacgcca ccacccgcaa cggagccaga
     1981 gggagctgtt ttcatggtct gctgactgat atctgttgag ggaacagttt gttttgagac
     2041 aagtttaggt gttttgactg gttgaatgtt gcgatcaact tcaacgtcgt aggtggatcc
     2101 attgacattc acttcaacat gtgcgtcttc aatctcgcca atttccacct cgtatttggt
     2161 tccgtttatt ttaaaagaaa attgtttcat ttttccgtgg tttactttct atgcctttct
     2221 ggcatttgtc tttgattata tagttttgaa ctccagggtg aatacgtctt attgacccgc
     2281 tgaatggtca gcacggtttc ttccctgtca tgcagctggt tattatacag ggataaagcc
     2341 agtgcaatag cagcgtttat ttctccgctt aattcagatt cttcctgtgt ttgaagctga
     2401 atctgacccg cacttacatg ttttttcttt ttcaccgaag ctttcagatc acctttaaac
     2461 aatttcgaaa tcagtttata caaaagatat aatagcgcaa gagccgacaa cactaccgac
     2521 atggctataa ttgccatacc aatgcccagg ggatcgtatt tgacaaacaa ttcagcattt
     2581 tttacaggtt caatcaatac attggattgg tttggtgtat atgctttcga gagaacccat
     2641 ttaccatcac catcaacgtc tcttgccaga atctgatcgg tttctgtaaa gttaaatgaa
     2701 acggaatcaa taattgtttg agcattcgat gcaaaaatgt aaatacaact ggttgagtca
     2761 agttgaaaat tggtgtaaaa aacgccgtgc tgagaaaacc cgttgcaata aaaaagcaaa
     2821 tagctacgtg aagccatata catgcgcgag tcagcaggaa ttcggtattt tttcggctga
     2881 tgaatatcat cagtcaaata cattccggca atatttatgg ttccataact actattgaat
     2941 atttcaatcc agggagaatg atgtccgaaa ccgtcaacag gaccgttgca gttcagcgtc
     3001 atcacttcgt ttatccggat atcggtttga ttttgcgcat tgataacctg aatctgaaaa
     3061 aaacacagaa gcaatgcaat ttgaaaaact ttgttcattg atcctaatcg ttagagaggt
     3121 agattactgt gtttttttgc aggcaggttt tgttttttcg tagccagtga ctgaagagcc
     3181 ctgattatgc gaaatcgggt attgcgaggt tcaataatgt catcaataaa gccataacgc
     3241 gcagcctcgt agggagatgc aaatttgttg cggtattcgg tctctttttc ttctatgaat
     3301 tttgcccgtt ctgcatcatt tgtaatttct ttaattttcc tgccttcaat aatttcaact
     3361 gcgccccggg ctcccatcac agctatttca gctgatggcc aagcgtaatt gatatctgca
     3421 cggagttgtt tggatcccat tacatcgtga gcaccaccat aggatttacg gagagtgatt
     3481 gttattttcg gtacggttgc ttcaccataa gcataaagca attttgcacc attgattatg
     3541 atgcctccat attcctgttg agttcctgga aggaatcccg gaacgtcaac caaagtaata
     3601 atcggaatat tgaaggcgtc gcagaaacgt acaaaccggg ctcctttttt tgatgcatca
     3661 atatcaagta ctcctgccaa atagcatggc tgattggcta caacgccaac agacattcca
     3721 cccaaacgtg aaaagccaac cactatattt ggtgcaaaat gtttatgtac ttcaagaaat
     3781 tccccgttgt cgacaaaacc gttaatgata tcttttacat cgtaaggttt gtttgggtta
     3841 tccggaatta tataattgag agaatcttca agtctgtcaa tgggatcttc gcactctgag
     3901 aatggtgcat cttccatatt gtttgatggg agatagctga gcagcttccg aattatttgc
     3961 attccttctt cttcgttttc agcaacaaaa tgtgaaacac cagatttggt tgcatgaata
     4021 ttggctccac cgagttcctg atcagtcaca acttcacttg ttacggtctt cactactttc
     4081 ggtccggtga caaacatgta gctgttttct ttcgacatca gaatgaagtc agttaacgca
     4141 ggtgaataaa cagcaccgcc ggcacagggg ccaaaaatgg ctgaaatttg cggaaccact
     4201 cccgatgcca taatgttgcg ctggaaaatg tcggcatagc cacccagact tttcacgcct
     4261 tcctgaatcc gggctccgcc ggagtcgttg atgccgataa ccggtgcgcc gactttcatg
     4321 gcctgatcca ttattttgca aatctttccg gcataggctt cagaaagtga cccaccgaat
     4381 acggtgaaat cctgggaaaa tacataaacc aaccggccat ctattgttcc ataacccgta
     4441 acaacaccgt ctgacaggta tcgctcgtct tccatgccaa aatcatggca attatgagtc
     4501 acaaacatgt caaattcttc aaagctacct tcatccagca gcatcgaaat gcgctcgcgg
     4561 gcagtcagtt ttcctttggc atgttgcgaa tcaatccttt tcaaccctcc tccttgacgg
     4621 gctttctcac gaagtcccat cagttcgctg attttttttt caatagacat attacttggt
     4681 atttttattt tcacaaaatt cggtgagtac gcctatggtc gatttcggat gcaggaagcc
     4741 aatggtcagc ccctcagcgc ctttacgggg cgttttgtca atcagccgga tacctttctc
     4801 ctcggcttcc gtcagcgctt tttcaatgtc ttttacagca aatgcgaggt ggtgaatgcc
     4861 ttcgcctttg ttttcaatga attttccaat cggtccttcc ggatctgtac tttccaataa
     4921 ttcaatcttg gtttgtccta ccatgaaaaa tgcggtttta actttctgat ctgccacttc
     4981 ttcgatggca taacatttca gtccaagaac ttcttcatag tactttatgc tctctgtcag
     5041 acttttaacg gcaatcccga tgtgctcgat atgagaaatt tccatgtttt tttttgcttg
     5101 caaatgtaac ggctattttc tttgataacc aaataatttt gcatttttaa acagatattt
     5161 ttttatttgc atttgcgcaa atcaataatt tggtcgttgg tgtgttaatt atttgttttg
     5221 agaaataaat ttcacatttc cagaatattt tttttgcgta gagtttgctc atttgttaat
     5281 aatatgtttg aattgtttgc aatatcttgt ctgtcagttt tttagtaatg cagttatgat
     5341 aattatcagc ttcctgattt catatttaat gtatttgtaa ttttggtacg tgaataccta
     5401 aatgaaaatc tatatgaaac gacagataat agaaatcgac gaagaaaaat gcaatggttg
     5461 cggaatctgt gccagcggct gtcacgaagg cgctttgcag attataaacg gtaaagccgt
     5521 tttgataaat gatttgctct gcgatggtct gggggcctgt attggtggct gtcctgaagg
     5581 tgccatcaat atcaccgaac gtgaagctga accatacgac gaagataaag tgatggaaca
     5641 tcttcataaa aaaggaaagg atgttgtgat tgctcatctg aaacatctta aagatcataa
     5701 tgagcatgtg tggcttcaca aagcagttga atggatgcag aaaaatcagg accggcttga
     5761 ttttgatatg atcgaagtgg tttatgttgt tcataatcat cacaagcagg gcgattcctg
     5821 tggttgttcg ggatcggctc atcgcgagat tgagcatgac acaaaaaatg aatcagtttt
     5881 tgccgaacaa tcatccacgc tggagcagtg gcctgtgcaa atgcatctta tcaatccgct
     5941 gtcttcggtt tttcataatg ccgatttgct gatggctgct gattgtgtgg ctttcagtat
     6001 gggtaatttt cacagcaaat ggctcaaagg gaaaaaactg gccattgctt gtcccaaact
     6061 tgacagcaac aaggaatcct atgtggaaaa acttacatca atgattgacg atgccaaagt
     6121 aaatactatt acggtgatga tgatggaggt tccttgttgt catagtctga ttcgtctggt
     6181 tcaactggcg cttgaaaacg catcgcgtaa agtgcctgta aaagccgttg tagtaagtgc
     6241 ttcgggcgaa gtgctcagcg aagaatgggt ttgattagag gtcagtagcc agtagccagt
     6301 gttcagttgt tatgagttat gggttatgag ttatgggtta tgagttatga gcgttttctc
     6361 agagcttatg actgatatct gaaagctgat atcactattg taacgattgt aacgcttgta
     6421 acgactgtaa cgcttgtaac gtgtaacgaa agcaaaatct attcatacaa aactactggc
     6481 ttatctgaaa cacagagagg gagccatgta tcaagtaatc cagcagctgc cagatcggtt
     6541 tttaaatgtt caaattctcc ggtgatctga tccgccatgt gcgcatcggc gctcagcatg
     6601 acgcgaacat tattttcttt cagaaaacca aaaatgtatt ttccgggata ataatcggta
     6661 tgcagcttgc gataaatgcc acgtgtgttg atttcgacaa tcacattttt ttcggcagcc
     6721 agcaatagag tttctttgag gagattgagg tgaaatgggt cttcagcatt gatgcggcgg
     6781 ttgcggttgt gcatcagaac cttgtcgcaa tgtcctaaaa tatccggttt ctgcgtttca
     6841 atcatttcgc aggtttgccg gtaaaatgat ttgactgctg cgcgcatgtc gtcgccaaaa
     6901 atttctttca gcccaatatc atagccttcg gccggaccat cgataaacca gagctggcct
     6961 ttgttttcga ccagatgaat ggagccgatc agatagtcga ggctgaagtt atttctccag
     7021 taatcaaatg gttttgtgat agcgggaatg taatcagctt cgagtccggt ccagattttc
     7081 gtactggtgc aggtattttt ggctgcatca atttcagcac aatattcatg tactttttca
     7141 tcggaaacaa tgctccattt gttttcaaaa ggtacaggcg catgacccga aagtccgaaa
     7201 tgatcaagct tttcgctgca ggcagccgat atcatttctt gcaccgacgc ttttccgtcg
     7261 caataggttg tatggttgtg gagactgtac atgatgcaaa tatattggtt ttgtcgatac
     7321 cctaaaacag gttaaggtta agattgagag gcgtagccgt cagagaagct ccggagcacc
     7381 attggatgcg cctccgcctc agcggggtaa attccggcgc aatagctgtt agtcttgggc
     7441 agaatgcctc aggtacaagg tgtgcgacct gtcacagctt gccatcacgt aaaacgtgac
     7501 aagctgtttt acgtggttgc aagccagaga cgtgcgccca gatttgcatt ttggcaaaag
     7561 gaattcggtt aagaaaattc caaaaatttt tgagaaatat atttagaaaa aaaatgtcga
     7621 aacacatgat atacatcatt ttatcaacaa acgcatgatt taatcagcat ttcagttgtg
     7681 aatatacgag tagagcaatg ctaattaaca tactttaaca ttcgacgaag caaattcttt
     7741 cacaaacgaa taaacttttt agtatcttta tatgtctaaa ctacgtgagg cgaaaaatcc
     7801 tgttaattaa tcaactacaa atttttcagg attattgcgg atcgatgctt gtaaaacgtt
     7861 ttaactacga aaaatgactg ttcaacctca aaaaactaaa aaaatgaatc tcttgaaaca
     7921 aaatatctac tggctgattt atcttgtcgt aatcggagcg ctgcttgctc ttgcaattat
     7981 cttcggataa tatagtgcat ttcacagaca gcagcggcat tgaatgagta agactctcag
     8041 catcggatag ttaatgctgt ctgtagtaga aattattttc taaacggaat cctgcggggc
     8101 ggggttccgt tttttatatt atcgcgcgga gctcgcaaag acgcagagaa attctgccac
     8161 aaggtcacca aggcacaaaa agaataatga acatggaacc tgtctgcaac aggcaggcaa
     8221 ggaattataa agtaagaagt aaattctttc attattctca cttcctcttg atccgtaagg
     8281 atgcacggat tgttcagtgt tcagagttct ttgggtgccg ggatctcctg gaaaaacctg
     8341 cgcagcccgt acactctttc ttccgcgaat gaacgcaaat cttcgcgaag aaacttgttt
     8401 ccctcagcag gtcttatgaa cgctaacccg ccagcatcag tcactatcgt gacatgatgg
     8461 gcggacaagt tcgaatcacc cttcatctgc gatgaagctc gattcagttg ggtcgcgcgg
     8521 ggaatggcgc gcagagggcg cagaggcgca gaggaattgg tcacaaaggc acaggacgat
     8581 ataagatatc agatcggtga tttttgattt ttgatctgaa gggtgaagtg ctgatttatt
     8641 tttgaagtat tgaatgctga aaatcttttg aagtaccttt ctactttaca aacctgataa
     8701 acttcccgac ttcatacgct catcaaaatt agtgcggcag caaaatttca atttattgat
     8761 aatgtgcgga ataaagattg tgatagttac attttaccgc actaacgacc tgtttttaat
     8821 aacatgaaaa agcaacaata tgaccatttt ctgtattttg acttcgggat gtcgcctaac
     8881 attttgcgga tcagaaatcg atgaaaagaa gcgtgggcgc cgaaggcatg atgcctgaaa
     8941 tatgtttgag ctggaataat ataaacagtt gttccatgca gcgagttatt tcaggctgtg
     9001 ggggggtagt tcatcgattt cccgcaaaat gttcaagcga cgagcttttc ttttgatact
     9061 tttcttttgg tgggcaaaag aaaagtattc tcagccggta ggctgtgcta aaaaaactgg
     9121 aaagcttaag cttccgggaa cgcagttgag gcaccgcact aactgtatga agtcaggaga
     9181 tatcagatcg gtgatttttg atttttgatc tgaagggtga agtgctgatt tatttttgaa
     9241 gtattgaatg ctgaaaatct tttgaagtac ctttctactt tacaaacctg ataaactttc
     9301 cactactgag ttcccagtca ttgattgtga tttagcttct gatgctgagc cgggatcttc
     9361 tggaaaacct tcacagccat tgctatcttt tgtccacgga tgacacggaa agctcggatt
     9421 aaaatacctc agcaggtctt tggatttcag cggatgaaat gcctttcagt ctgcgactga
     9481 agccatatcc acggatatgt gcatgaatct tttacagtca atgattatta cgcagtaata
     9541 attgttgacg acagtgtaat cgaggtgggg ccgctgacta aaaatctctt tcagtgtaat
     9601 ccgcgtaata tgtggactat attgtgttag ccgggcgtac tgagttcccg ggcaagccaa
     9661 acgaatattg aacaatgaac aaggattttt tgaagtatga agtgaagttt gaagtgaaga
     9721 attcgtcgac gttctcactt ccttgttcca tgttcagagt tcacatgtgc cgggatctcc
     9781 tggaaaacct tcacagccct tgctgcgcac gcgaaggaga gactccggat ccacgtgaaa
     9841 cgtggcaaac aagtccgggg acttggtagt cagggtctgg cgtgctgcga tgaagactga
     9901 ttcagtgggt cgaagagagg gtgcaggaga attgcgcgcg gagggcgcag ggacgcagag
     9961 aaatttggca tgaaggcacc gaaatttgaa gaaaactcaa agtcgaaata gcttttatat
    10021 gtatccgacc aagatcaccg atcgctgctc accgctcgct ggttaccgta gaagcttgat
    10081 cttgatctca acctcgtctt tgacctcgac cttgtccttg aagttttttt gttaaatttc
    10141 ccgccggaaa aacgcctgca tatcgcactg tgatttgatc tggtcaacat acaacgcgca
    10201 atccgcgcgg atatcgggag cgttatgcaa acaatgaata acctgtgtta aaatcgctgt
    10261 taataacttt a
//
LOCUS       DAOPKE010000096         9967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7111_length_9967_cov_9.747074, whole genome shotgun sequence.
ACCESSION   DAOPKE010000096 DAOPKE010000000
VERSION     DAOPKE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7111_length_9967_cov_9.747074"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..915
                     /locus_tag="PKY63_10560"
     CDS             373..915
                     /locus_tag="PKY63_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01099.1"
                     /translation="MRKIEKKWELYRPEGVSKTEDGKDVDKKQLFELQPDLNEVKFNN
                     HLVGLIDENVVGDQNIETQKQLAAEKKIFSRNKWVKIKYRKKAIFRIVKGTVAAGFTE
                     KHIWLSYDSRIALGIRDNKEGKSLKLLTVKKCNRFESLIWAPLHAADPILKAQYRLAT
                     FLAVLGVVISVIGLVISLNS"
     gene            1027..2037
                     /gene="pseB"
                     /locus_tag="PKY63_10565"
     CDS             1027..2037
                     /gene="pseB"
                     /locus_tag="PKY63_10565"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HPF01100.1"
                     /translation="MLNGKSILITGGTGSFGKMFVKTILENYPEVTRLVVYSRDELKQ
                     YEMAQQFPASKYPQVRFFIGDVRDYPRFKRACQGIDVIIHAAALKQVPTAEYNPDECI
                     KTNIGGAQNVIDAALGSDVKIVVALSTDKAAAPINLYGATKLVSDKLFIAANNIKGKR
                     DLRFSVVRYGNVMGSRGSVIPFFMQKAKEGVIPITHKEMTRFNISLEEGVEMVLWAIE
                     NAIGGEIFVPKIPSYTIETVAKAIAPNAKLEYIGIRAGEKIHEEMITDSDSYNTIEFD
                     RYYAILPTDANKEKFLKHYNAHEVEKGFRYNSGTNAEWETIESMREKIVKFVDPNFKP
                     IE"
     gene            2038..3189
                     /gene="pseC"
                     /locus_tag="PKY63_10570"
     CDS             2038..3189
                     /gene="pseC"
                     /locus_tag="PKY63_10570"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HPF01101.1"
                     /translation="MKNIPYGRQNITQSDIDAVSEVLRSDFLTQGPEIEKFEKAFAAY
                     IGSKYAVAVANGTAALHLCALALNVKPGEKVITTPITFAASANCVRYAGGEVVFADID
                     PTTYLLDINSVEELLKSAPKGTYKGIIPVDFAGRSVNTEAFRKLADEYGCWILEDACH
                     APGGYFVDSKSEKQHCGNGVYADLAIFSFHPVKHIAAGEGGMITTNNEELYKKVSLLR
                     THGITKDPALLHENHGGWYYEMQELGFNYRLTDFQAALGNSQLQRADEGLKRRREIAA
                     KYNTAFAGKNFIHGQSGDIDGHAYHLYIVEVDDRKGLYDYLREHKIFAQVHYIPVHMQ
                     PYYRDLGWKKGDFPHAEKYYEQCLSLPMYPTLSDEEQNFVIETILNYYK"
     gene            3186..3881
                     /gene="pseF"
                     /locus_tag="PKY63_10575"
     CDS             3186..3881
                     /gene="pseF"
                     /locus_tag="PKY63_10575"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HPF01102.1"
                     /translation="MKNLAIIPARGGSKRIPRKNIRDFFGKPIIAYSIQAALSSGLFD
                     EVMVSTDDDEIAEIARQYGAAVPFMRSENTSDDFATTSDVLIEVIESYKLQGRVFENV
                     CCIYPAAPLIKSTHLTEGYEKLINEKRNSVFPVVAFSYPVQRGLIIDSEGRTNMLHPE
                     NASVRSQDLQKVYHDAGQWYWLNTESFLADKKIFSSNSASVILSETEVQDIDTLIDWK
                     MAEFKYEYLQSLT"
     gene            3856..4857
                     /locus_tag="PKY63_10580"
     CDS             3856..4857
                     /locus_tag="PKY63_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF01103.1"
                     /translation="MNTYRVLHKQTFVSGQYSIVPIRMEDRWLIMQWRNEQIYHLRQQ
                     EPLTEDAQDAYFNNVISALYDQRMPSQVLFSFLENGSFIGYGGLVHIDHEKLTAEISF
                     IMDTALEKEFFVHHWSTFLSLIEKVAFEELGLHTIFTYAYNLRPRLYEALEACGFKLE
                     SKLPAHVEIDGVMTDVLIHSKRASYYLRSATAADVDITFKWASDKEIRKYSFSQQKIE
                     YSEHERWFTNKINDASCEYYILCSIDKCIGSIRFEMNEKNEAMISYLIDSGFHGKGFG
                     TAILKLGIQMIRWKKPHINSVYGMVKSENPASVRIFEKLGFTASEADSGTLRFDKTL"
     gene            4860..5903
                     /gene="pseI"
                     /locus_tag="PKY63_10585"
     CDS             4860..5903
                     /gene="pseI"
                     /locus_tag="PKY63_10585"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HPF01104.1"
                     /translation="MKIGNRDISEAAGVFIIAELSANHNGSFETAVETIRAAKHAGAD
                     CIKLQTYTPDTVTIDCRKPDFMIRGTIWDGKNLHDLYSEAYTPWEWHEALFRTAEEEG
                     LICFSSPFDKSAVDFLETLNAPAYKIASFEITDIPLIEYVASKGKPVIISTGIASEED
                     IALAVDTCRRAGNNDIALLKCTSSYPAPVAEANLRMMQDIAERYHCITGLSDHTMGST
                     VPIAAVALGARIIEKHFILDRSAGGPDASFSMNVEEFSDMVKAVRDAEKSLGKVDYSL
                     SEKQIQSRAFARSLYVVKDIKAGEVFTEENVRSIRPGYGMHPKYLKEVIGKVAKKDLR
                     KGTPLSIELISEK"
     gene            5893..7149
                     /locus_tag="PKY63_10590"
     CDS             5893..7149
                     /locus_tag="PKY63_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPF01105.1"
                     /translation="MKNRLQFGDFGKVIARYASAQIASNFLRLLSGFLVVSFIDPDLY
                     GQFSGIGVFLGYILLGHGGIVNGLSRELPYELGRNNDQYAKQMASSVYVLSVAICFVA
                     SLSFLGFGIKFLLQGDTTTAFIYFAYTIIGGLHLLNKQFLPTLYRTNKDFDSLSKQNI
                     SVGLGNVITVILVYFFSLYGLIARGIILALLEFYLLFKNKPYKLSFVYHLKHFRTLFK
                     TGLPIFMVGQVGPIWNTIMNNIIFSGSGALNFGLYSLSTIIQGAFGVIPHSFSQVIYP
                     RMSIMLGQGKSVSYIVRSSLKPIVFQFFVMAGISIIGVLLLPIVIPIILPKYTEGILA
                     AQWMLFVPVAQSFGALNNIYNVVKKQKWYFLSLLTGAIVGSLYIYWKVQSGNFQLEYF
                     PQGILIGTIIQQVLSLLFLSTIKNND"
     gene            7415..8485
                     /locus_tag="PKY63_10595"
     CDS             7415..8485
                     /locus_tag="PKY63_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01106.1"
                     /translation="MQTTLSDSDLINIKTLYISPHANQNVNRDVFFFSNIFIFFKIAF
                     FLICCLHAFNKKIKPLNLSLFFKLNLLFDLHLQLVKSLSLKKFVQIQSKTKLIGADYD
                     RGSDSSLFFAVSKSLNIKSFTFQHGVINPPVGYSPVNADEIWVWGGMAKMQLMELNVP
                     EVKIRITGTPIVQDIEISNEIKQSAEIRYGFKHGSNIVLALSVPNKQNDIRLVEFLYH
                     IKSEYGDENYNFCVKIHPARNYEEYKWIKDIYNIDILPHDIPYQDFINFVDVLLSHSS
                     GLAAEVLFFKKKVGILDILDESPGNGIELNKYFRVSLIQQASDFEKLINDPTTNISET
                     LFYKVGYDAKKEIQKNIEYLIA"
     gene            8571..9155
                     /locus_tag="PKY63_10600"
     CDS             8571..9155
                     /locus_tag="PKY63_10600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPF01107.1"
                     /translation="MIKKYIIYGYRLFWWSINLWRFKKLGMYSYINRALKIDGAKNIS
                     IGRKVTIQYKTWLASRPLTANNSPILEIGDGSAIGNFNHIYSTNKIIIGKKVLTADKV
                     YISDNIHGFLNPLVPIMNQPIIQKNAVIIGDGTWLGENVCVIGAKIGKNCVIGANSVV
                     TKDIPDFCIAVGAPARVIKRYNFETQEWEKTNKK"
     gene            9174..>9967
                     /locus_tag="PKY63_10605"
     CDS             9174..>9967
                     /locus_tag="PKY63_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPF01108.1"
                     /translation="MKNILITGGAGFIGSNLCEYFLDLGHTVTCLDNFSTGHHKNIEG
                     FLPNENFSLIEGDIRNLDTCHKACEGVDYILHEAALGSVPRSVNDPINTNDNNINGFL
                     NMLVAARDANVKRMVYAASSSTYGDSTELPKVEEHIGMPLSPYAVTKYVNELYAHVFH
                     NVYGLDIVGLRYFNVFGRRQDPNGAYAAVIPKFVSVLMAHESPVINGDGTVSRDFTYI
                     DNVIQMNHLALASDNPAVSGQVFNTAVGERNNLNQLVSYLKELLSA"
BASE COUNT         3078 a         1833 c         2127 g         2929 t
ORIGIN      
        1 taaaacatct cgtatttctg atctcgtatt tcgtctttcg tatttcttaa tctcctgcgc
       61 aaagcgcatg aaaaaagtta agcacgaagt gcctaaatta atctttcgcg aagcgaacca
      121 ataaaacaac tgacacagcg atgcagcgat gaggcgataa tgcgatgatg cgacaccacg
      181 cccacgcgca tcaacgccca cacgccacat cgatcttcac ttccgcgcaa agcgcataat
      241 aaaacatctc gtatttcata tttcgtctct cgtatttttc tccatcccgc tcccggacaa
      301 tgatttgaag aactatcaat attacagtaa tcaagcacac attcaaaata attattcaaa
      361 acaaaaccaa tcatgagaaa aattgaaaag aaatgggaac tttatcgtcc agaaggtgtt
      421 agtaaaactg aggatggaaa agatgtagat aagaaacaat tatttgagct gcaaccggat
      481 ttgaatgaag tgaaattcaa caaccatttg gttggtttga tagatgaaaa tgtcgtaggt
      541 gatcagaata tagaaacgca aaaacaactg gcggccgaaa agaagatatt cagcaggaac
      601 aaatgggtaa agataaaata tcgcaagaaa gcaatcttta gaattgtaaa ggggacagtt
      661 gctgctggct ttactgaaaa gcacatctgg ttgagctatg attcaagaat tgctttggga
      721 ataagagata ataaggaagg gaaatcttta aagttgttga ctgtcaaaaa atgcaaccgc
      781 ttcgaatccc tcatctgggc gccgctccac gctgccgatc caattctcaa agcacaatac
      841 cgcctcgcaa ctttcctcgc tgtactgggt gtggtgatct cggtgattgg attagttatt
      901 tctttgaaca gctaggttaa ttttaaaatg ctttgggaat tgaattcagc aataataatt
      961 acttttgcac attcaaacgc ctctctgagg aaaaccaatc gaaaaaataa ataaaaaact
     1021 gatattatgc taaatggaaa atctattcta atcacaggcg gcaccggctc tttcggaaag
     1081 atgtttgtga aaacaattct tgaaaactat cccgaagtta cacgactcgt tgtgtattca
     1141 agagatgagc ttaaacagta tgaaatggct cagcaatttc cggcttcaaa atacccacag
     1201 gtaaggttct ttattggtga tgtaagagat tatcccagat ttaaaagagc ttgccaggga
     1261 attgatgtta tcattcatgc tgctgctttg aagcaggttc caacagccga gtacaacccg
     1321 gatgaatgca ttaaaaccaa cattggaggg gctcagaatg ttattgacgc agcacttggc
     1381 tcagatgtaa aaattgttgt tgcattatca actgacaaag ctgctgcgcc catcaattta
     1441 tatggagcca caaagcttgt ttctgacaaa ttgttcatcg cagcaaataa tatcaagggc
     1501 aaacgtgatt tgcgtttctc cgtggttcgt tatggaaatg tgatggggtc tcgtggatca
     1561 gtaatcccat tctttatgca gaaagcaaaa gaaggtgtta ttccaatcac acataaggaa
     1621 atgacacgat tcaatatttc tcttgaagaa ggtgttgaaa tggttttatg ggcaattgag
     1681 aatgccattg gtggcgaaat ctttgttccg aaaattccgt cctataccat tgagactgtt
     1741 gcaaaagcca ttgcccccaa tgcaaaactt gaatatatag gtatccgtgc cggtgaaaaa
     1801 attcatgagg aaatgattac cgacagtgat tcatacaata ccattgagtt tgacagatat
     1861 tatgctattt tgcctactga tgccaacaaa gaaaaatttc tgaaacacta caatgctcac
     1921 gaagtggaaa aaggtttcag atacaattca ggtactaacg cagaatggga aaccattgaa
     1981 agcatgcgcg aaaaaattgt gaagtttgtt gatccaaatt tcaaaccgat cgaataaatg
     2041 aaaaatattc catacggacg acaaaacatc actcagtctg acattgatgc ggtatccgaa
     2101 gtgctgcgct cagactttct gacgcaaggt ccggaaattg aaaaattcga aaaggcattt
     2161 gctgcgtaca ttggatcgaa atacgctgtt gctgttgcaa atggcacagc ggcgctgcat
     2221 ttatgtgcgc ttgcattgaa tgtaaagccc ggtgaaaagg tgattaccac accgatcaca
     2281 tttgctgctt cggccaattg cgtgcgatat gcaggtggtg aagttgtgtt tgccgatatt
     2341 gacccgacaa cttatctgct cgatataaac agtgttgaag aacttctgaa atcggctcct
     2401 aaagggactt acaaaggtat cattccggtt gatttcgcag gacggtcagt caatactgaa
     2461 gcattcagaa agcttgcaga tgagtatgga tgctggattc tggaagatgc ctgtcatgcg
     2521 ccgggaggat atttcgttga ttcgaaaagt gaaaagcaac attgcggtaa tggtgtatat
     2581 gccgatctgg ccatcttttc ctttcacccg gtaaagcaca ttgctgctgg tgaaggcggc
     2641 atgattacaa cgaacaatga agagctttac aaaaaagttt cattgctgcg cacgcatgga
     2701 ataaccaaag atccggcgtt gctccatgaa aaccatggtg gatggtacta tgaaatgcag
     2761 gagcttggat tcaattaccg gcttacggat tttcaggcgg cacttggcaa cagtcagctg
     2821 cagcgcgccg atgaaggcct gaagcgccgg agagaaattg ctgcgaaata caacactgct
     2881 tttgcgggaa agaattttat tcatgggcaa tcgggcgata ttgatggcca tgcctatcat
     2941 ctttatattg ttgaagttga tgacaggaaa gggttgtatg attatctgcg cgaacataaa
     3001 atatttgccc aggtgcatta tattcccgtt catatgcagc cctattaccg cgatcttgga
     3061 tggaagaaag gcgattttcc gcatgctgaa aaatactatg agcaatgcct gagtctgccg
     3121 atgtatccga ctttgagcga tgaggagcag aattttgtta ttgaaacaat tttgaattat
     3181 tacaagtgaa aaatctggca attataccgg ccagaggcgg cagtaagcgc attccgcgaa
     3241 aaaacatccg ggattttttc ggcaaaccca ttattgcata ttccattcag gccgcattat
     3301 catcaggact ttttgatgaa gtgatggttt cgaccgatga tgatgagatt gctgaaattg
     3361 cccgccaata cggggctgca gtgcctttta tgcgttctga aaatacatcg gatgattttg
     3421 caacaacaag cgatgtgttg attgaagtca tcgaatcgta taaattgcag gggcgggttt
     3481 tcgaaaatgt ctgctgcatc tatccggctg cacccctcat caaatcaact catctgaccg
     3541 aaggttatga aaagctgatc aatgaaaagc gcaattcggt ttttccggtc gtggcatttt
     3601 cttatccggt gcagagggga ttaatcattg acagcgaagg aagaacgaac atgcttcatc
     3661 ccgaaaatgc atcggtgcgc tcgcaggatc tgcaaaaagt gtatcacgat gcagggcagt
     3721 ggtactggtt gaatacagaa agttttcttg ctgacaaaaa gatattttca tcaaattccg
     3781 cttcagtgat tttatccgaa accgaagtgc aggacattga tacgctgatt gactggaaaa
     3841 tggctgaatt caaatatgaa tacctacaga gtcttacata agcagacatt tgtttcggga
     3901 cagtattcca ttgtgcccat acgaatggaa gaccgctggc tgatcatgca atggcgcaat
     3961 gagcagatct atcatctcag gcagcaggag ccgcttacag aggatgcgca ggatgcctat
     4021 ttcaacaatg tgatttcggc attatacgat cagcggatgc catcgcaggt attgttttcg
     4081 tttcttgaaa atggctcgtt cattggctac ggagggctcg tacatataga ccatgaaaag
     4141 ctgacagcgg aaatttcctt tattatggac actgctcttg agaaggaatt ctttgtacat
     4201 cactggagta cttttctgag tctgattgag aaagttgctt ttgaggaact cgggctgcat
     4261 acaattttca cctatgccta caacctgcgt ccgcggctgt atgaagcgct tgaggcctgt
     4321 ggtttcaaac ttgaaagtaa actgccggcg catgttgaaa tcgatggtgt gatgactgat
     4381 gtactgattc attcaaaacg cgcgtcatat tatcttcgtt ccgctaccgc tgcggatgtt
     4441 gacatcacct tcaaatgggc ttcggataag gaaatccgga agtattcatt cagtcagcaa
     4501 aaaattgaat acagcgaaca cgagcgttgg tttacaaata aaataaatga tgcatcgtgt
     4561 gagtactaca ttttatgcag tattgacaag tgcatcggat cgatccgctt tgaaatgaat
     4621 gaaaaaaacg aagcgatgat cagctatctc attgactccg gatttcacgg aaagggcttt
     4681 ggaacagcta ttctgaagct tggtattcag atgatcagat ggaagaaacc acatataaat
     4741 tcagtgtacg gaatggtaaa gtctgaaaat ccggcctcgg tcaggatttt cgagaaactt
     4801 ggctttacag catcagaagc cgattccggt acattgcgtt ttgacaaaac actttgattg
     4861 tgaaaatcgg aaacagagac atatcggaag cagccggcgt ttttattatc gctgaattgt
     4921 cggccaacca caacggcagc tttgaaaccg cagtcgagac catacgtgca gctaaacatg
     4981 ccggagccga ttgcatcaag cttcagacat atacacccga taccgttacc attgattgcc
     5041 gcaaacctga cttcatgatc aggggcacca tctgggacgg aaaaaatctg cacgatctgt
     5101 attccgaagc atacacgccc tgggaatggc atgaagcgct tttcagaaca gctgaagaag
     5161 aagggctgat ctgcttttca tcgccgtttg acaagtcagc tgttgatttt ttggaaacgc
     5221 tgaatgcacc ggcctataaa attgcatcgt tcgaaatcac cgatattcct ttgattgagt
     5281 acgttgcttc taagggaaag ccggtaatca tttctaccgg aattgccagc gaagaagata
     5341 ttgcgctggc tgttgataca tgtcgcagag cggggaacaa cgatattgcg ctgctgaaat
     5401 gcacatcgag ttatccggcg cccgttgctg aagccaatct ccgaatgatg caggacatag
     5461 cggagcgcta tcattgcatc acaggattat cggatcacac catgggaagc acggttccga
     5521 ttgcggctgt ggcattgggc gcgcgcatca tcgaaaaaca tttcattctt gatcgttctg
     5581 caggcggccc cgatgcttcg ttttcgatga atgttgaaga gttttccgat atggtgaaag
     5641 cggtccgtga tgcagaaaaa tcattgggca aagtggatta ttcgctgagt gaaaaacaga
     5701 ttcagagcag ggcttttgcg cgatcgctgt atgttgtgaa agacatcaaa gccggtgagg
     5761 ttttcacaga agaaaacgtg cgcagcataa gaccgggata tggaatgcat ccgaaatatt
     5821 tgaaggaggt gattgggaaa gtggcgaaaa aggatttgag aaaaggaaca ccgttatcga
     5881 ttgagttaat cagtgaaaaa tagattgcaa tttggagatt tcgggaaagt gattgcacgc
     5941 tatgcgtctg cccagattgc ttcaaatttt cttagattac tatcgggctt tttagtcgtc
     6001 agttttattg accctgattt atatgggcaa ttctctggta tcggtgtttt tctggggtat
     6061 attttattag ggcatggtgg aattgtaaat ggtcttagcc gagaattgcc ttatgagtta
     6121 ggtcgaaata acgatcaata tgccaaacaa atggcatctt cggtgtacgt tctctctgtt
     6181 gccatctgtt ttgtcgcctc attgtcattt ttagggttcg ggataaaatt tctgcttcaa
     6241 ggcgatacta ccactgcatt tatttatttt gcctacacca ttattggtgg gctgcatctt
     6301 ctaaataagc agtttttacc aactttgtat cgaacaaaca aggattttga ttcactctca
     6361 aaacaaaaca tttctgtagg gcttggcaat gtaattacgg ttattctggt ttattttttc
     6421 agtctgtatg gtctcatcgc ccggggcatt atcttagcat tacttgagtt ttatttgtta
     6481 tttaaaaaca aaccctataa gctctccttt gtctatcatt taaagcactt tagaacttta
     6541 tttaaaacag gtctcccaat ttttatggtg gggcaggttg gcccaatttg gaatacaata
     6601 atgaataaca ttatttttag cggcagtgga gcgctcaatt ttggattata ctcattaagt
     6661 actattattc aaggcgcttt tggtgtaata ccgcactcct ttagtcaagt gatttatccg
     6721 cgtatgagca tcatgttagg acagggtaag tctgtttcat atatagttcg gtcaagttta
     6781 aaaccaattg tttttcaatt ttttgtaatg gcaggaatta gtattattgg tgtattatta
     6841 ctgcccatag ttatacccat tattttacca aaatataccg aaggaatttt agctgctcaa
     6901 tggatgctgt ttgttccggt tgcccaatcg tttggagcac ttaataacat ctacaatgta
     6961 gtaaaaaagc aaaaatggta ttttctctca ctattgaccg gcgctatagt agggtctctt
     7021 tatatttact ggaaagttca atcggggaac tttcagcttg agtactttcc gcagggcata
     7081 ttaatcggta cgataattca acaagtttta agtctgttat ttctttcaac aataaagaac
     7141 aatgactaaa aaaacaatta tacttgattc cagaaagttg cacgaagttg aatcggaact
     7201 caattcaatc aaatataagg gtctcgaagt tgggaatttg ttatcgtact attttgacag
     7261 attgtataga aatgattaca atttcaattg gttaaagaaa attgtggtta tcatttatta
     7321 ttcgataaaa cctgtctcaa aaaagcagat tttatttgat cataaaaaag aattaattta
     7381 tttccgtacc ggggatcacc ggcatcttaa aaacatgcaa actactttgt ccgactcgga
     7441 tttaataaat attaaaacct tatatatttc tccacatgcc aatcaaaatg taaatagaga
     7501 tgttttcttt ttttcgaata ttttcatttt ttttaaaata gcattttttt tgatttgctg
     7561 cttacatgct tttaataaga aaataaagcc acttaatcta tcattgtttt tcaaattaaa
     7621 tttattattt gatttgcatc ttcaattggt gaaatctttg tctttgaaga aatttgttca
     7681 aatacaatct aaaacgaaac tcatcggggc tgattatgat cgtggatcag attcatcgtt
     7741 gttttttgcg gtctcaaaat cactaaatat caagagtttt acttttcaac acggagtgat
     7801 taatcctcct gtgggatatt ctcctgtgaa cgctgatgaa atttgggttt ggggtggtat
     7861 ggctaaaatg cagttgatgg aactgaatgt tccagaagtt aaaattcgta taactggaac
     7921 accaatagtt caggatattg aaatatccaa tgaaatcaaa caatctgctg aaatcagata
     7981 tggtttcaaa catggtagta acatagtgtt agctctaagt gttccaaata agcaaaacga
     8041 cattagactg gtagagtttt tatatcacat taaaagcgaa tatggtgatg aaaattacaa
     8101 tttttgtgta aagattcatc cagcaagaaa ttatgaggaa tataaatgga ttaaagacat
     8161 ttataacatt gatattcttc ctcacgatat accatatcag gattttatta attttgtgga
     8221 tgtattattg tctcattcca gtggtcttgc tgcagaagta ctatttttca agaaaaaggt
     8281 tggcatttta gatatacttg atgaatctcc tggaaacggt attgaattaa ataaatattt
     8341 cagagtttca ttaattcagc aagcctctga ttttgaaaaa ttaataaacg atcctacaac
     8401 caatatcagt gaaactttgt tttacaaggt tggatatgat gcgaaaaagg aaattcaaaa
     8461 aaacattgaa tatttaattg cttagccaaa atgataaatc agaaagcaaa ttttcgaaac
     8521 cataatagac taattacgaa caaaagcaaa ctaaataggt caaatctcgc atgattaaaa
     8581 agtacattat ttatggatat agacttttct ggtggtctat aaacttgtgg cgatttaaga
     8641 aattaggaat gtattcatat ataaatcgag cactaaaaat tgatggtgca aaaaacattt
     8701 caattggacg gaaggtgaca attcagtata aaacatggct tgcttctaga ccattgactg
     8761 cgaataatag tccaattctg gaaattggag atggttctgc aataggtaat tttaatcata
     8821 tttattcaac aaataagatt attattggta aaaaagtatt gactgccgat aaagtttata
     8881 tatctgataa tattcacgga tttttaaatc cacttgtacc aattatgaat cagccaatta
     8941 ttcaaaaaaa tgctgttata attggtgatg gaacatggtt aggcgaaaat gtttgtgtaa
     9001 ttggagcaaa aattgggaaa aactgtgtaa ttggcgctaa ctctgttgta acaaaagaca
     9061 ttcctgactt ttgcatagct gttggagcac cagcaagagt tattaaaaga tacaattttg
     9121 aaactcaaga atgggaaaag acaaataaga agtaaaatag tcaaataatg aaaatgaaaa
     9181 acatattaat caccggtgga gccggattca taggatcaaa cctttgtgaa tattttctcg
     9241 atcttggaca cacagtcact tgtcttgaca atttttcgac aggccaccac aaaaatattg
     9301 agggattttt accgaatgaa aatttctctt tgattgaagg cgatatacgc aaccttgata
     9361 cctgccacaa agcatgcgag ggtgttgatt atattctgca tgaagctgca cttggctctg
     9421 tgcctcgttc ggtgaatgac ccgatcaata ccaatgataa caacataaat ggatttctca
     9481 acatgcttgt tgctgcccgc gatgcaaatg taaagcgcat ggtgtatgca gccagttctt
     9541 ctacctatgg cgacagtacc gagctcccaa aagttgaaga acacattgga atgccgcttt
     9601 cgccgtatgc tgttacaaaa tatgtgaatg aattatacgc gcatgtgttt cacaatgtgt
     9661 acggtttgga cattgttggt ctgcgctatt tcaatgtgtt cggacgcagg caggatccaa
     9721 acggcgctta tgctgcggta attccgaaat ttgtttccgt gctcatggca catgaaagcc
     9781 cggttataaa tggcgatggt accgtaagcc gcgatttcac ctacattgac aatgttattc
     9841 agatgaatca tctggcactt gcttctgata atcctgctgt gagcggccag gtgttcaata
     9901 cagctgttgg agaacgaaac aatctgaacc agctcgtgag ttatctcaaa gagctattat
     9961 ctgcata
//
LOCUS       DAOPKE010000097         9911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7156_length_9911_cov_18.790280, whole genome shotgun sequence.
ACCESSION   DAOPKE010000097 DAOPKE010000000
VERSION     DAOPKE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_9911_cov_18.790280"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3059
                     /locus_tag="PKY63_10610"
     CDS             <1..3059
                     /locus_tag="PKY63_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF01109.1"
                     /translation="VISVAASDANICVGENSVLTATSDIPGSSFLWSTGANTASITVS
                     PAATTTYNVTVTSPEGCVSTGEVTVNVFPNPVLTITASSGAICFGDPSVISVSSSIAA
                     TTFEWSTGETTANITVSPASTSTYFVTGTSANGCTGTASIEIIVNPLPVISVAASDAN
                     ICVGENSVLTATSDIPGSSFLWSTGANTASITVSPAATTTYNVTVTSPEGCVSTGEVT
                     VNVFPNPVLTITASSGAICFGDPSVISVSSSIAATTFEWSTGETTANITVSPASTSTY
                     FVTGTSANGCTGTASIEIIVNPLPTITTTVTDSIICDGESVTLTAFSDITGSTYQWST
                     GASTPEIIVAPNLTNTYYLTVTSPEGCVSTANSTITVNPNPIASIVPSSGAICMGSTA
                     TLTANSNIAVNSYLWNTAETNPSIIVTPATTSTYTVTLTTAEGCSGTADISVFVNPLP
                     DISVTAPAAAICIGSSLDLTANSNHPSTGYEWSTGQTTPSINVSPAASSTYSVTGTDI
                     NGCTGEAEFIVTVNPLPDINIVSAQNEICEGMSVELTATSNIPDTDFEWNTGQTSSSI
                     IVSPPASTTYEVTGTDANGCTGSNRITIQVNPNPTVTINPNSLTICPGDTATLNASSA
                     MAITYAWNTGATTTSISVWPSNTSSYSVTGTDINGCIGIAAATVVVRNAPVIVVSPYN
                     PKICYGDSVTLIASGAVLYDWTPATGLNTNVGHIVTSSPDSTTTYIVAGVDLYGCQGS
                     TNVTVTVHPIPTVDFRADETDICQATVVHFSGMASPSIQSWEWDFGDPASGASNSSSM
                     QNPMHAFFETGTYTIGLSVVTTAGCKNSIQKPSYIIVHPNPIASFARTPDITTLEYPT
                     IQFYDQSLGEKSRLWDFDDPGSGLDNNSIAKNPTHTYSASGEYWATLFVENEWGCLDS
                     VSNRIIINPTWDEYIPSAFSPNGDGNNDVFRPMGFNIDFTQYQMYIYDRWGKEIFDTD
                     NIEEGWDGRVNDHGETVQQDVYVYLIILTDISGLEHQFIGHVTVVK"
     gene            complement(3165..3728)
                     /gene="gmk"
                     /locus_tag="PKY63_10615"
     CDS             complement(3165..3728)
                     /gene="gmk"
                     /locus_tag="PKY63_10615"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPF01110.1"
                     /translation="MKKAVIFSAPSGAGKTTIVHRLIDAGLPLGFSVSATSRKPRTGE
                     INKIDYFFFSAEEFRKKVEQQNFLEWEEVYDGLFYGTLKSEVERVWNNGNAIIFDVDV
                     KGGINLKNIFGEKALSVFIQPPSFEILEERLRLRHTESEEAIQKRLDRAKYELSFAPE
                     FDVVIVNNELETAVSEALDIVSDFLKS"
     gene            complement(3732..4610)
                     /locus_tag="PKY63_10620"
     CDS             complement(3732..4610)
                     /locus_tag="PKY63_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPF01111.1"
                     /translation="MLRSMTGFGRVHTSIGNSTVAVMVRTLNNKQLDLSVKTPFRYRE
                     RENQIRDIAAKILLRGKVDINVIIEDVTNADASVNKTLAKKYFTELSVLSDHLGINKP
                     DNWMEILIRLPEVVGYGNPVVEEDEWLALFELVRKACEETDAFRMQEGAVLETDMIRH
                     LDVIEKLLPEIEQLDPERKIQVRERLVKELQQAQLDVKTDETRLEQELFYYIDKFDIN
                     EEKVRLAKHIEYFRNTLKQNDSEPAGKKLGFIAQEMGREINTLGNKSNFFDIQKRVVV
                     MKDELEKIKEQLANIL"
     gene            complement(4616..7282)
                     /gene="mutS"
                     /locus_tag="PKY63_10625"
     CDS             complement(4616..7282)
                     /gene="mutS"
                     /locus_tag="PKY63_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPF01112.1"
                     /translation="MVKKNFIFLLQRLNQVSVGSVAETPLMRQYNEIKVKYPDAILLF
                     RVGDFFETFGEDAVRASAILGITLTKRANGAASHIELAGFPHHALETYLPKLVKAGQR
                     VAVCEQLEDPKLTKKIVKRGITELVTPGVAFSDNVLDRKSNNFLASVFLHAPKTGASF
                     IDVSTGEFFVAEGSNEYIARLLQNMQPSEVLVKKTQKNEFLDVFGPFNVFAFDDWIFS
                     AQYAQDLLLRQFETENLKGFGVDTMENAVIAAGACLHYINETRQNGGSAHIRSVSRID
                     NDDYVWLDRFTIRNLELVQSYNERGRTLLSTIDRTSSPMGSRLLQRWLLLPLINKDKI
                     NDRLDAVSCFYENKELSFQVQQLLTEIADLERLCGKLAVGKINPREFLTLAYSIEKTK
                     ELCAIIPNEKDPLQKIVSRFANLDELSVLIKKYIREDAAALIVKGGVICPGINNELDE
                     LRLLSTDSKNFLSGIQQNEALRTGIASLKIGFNNVFGYYLEVTNTHKDKVPEEWIRKQ
                     TLANAERYITPELKTYEEKILGAEEKILRIEQDLYASLIQQSMPFTAALQKNASLLAI
                     IDVFLSFALIASENNYCRPVINDGLTIDIKGGRHPVIETQLPPGDKYVENDVLLNSDD
                     QQIIILTGPNMAGKSALLRQTALIVLMAQCGCYVPAASAEIGLVDKIFTRVGASDNLT
                     SGESTFMVEMNETASILNNLSLRSLILLDEIGRGTSTYDGISIAWSITEYLHNHPLCH
                     AKTLFATHYHELNELATYLPRVRNFHISVKESGNNIVFIRKLTPGGTEHSFGIHVARM
                     AGVPRNIIERAGEILVRLEKEHGSDISVGESNKTKTAAEPFQLSLIQLDDPLLENLRD
                     EILNTNVDMLTPVEALMKLNSIKKILEKNKKQ"
     gene            7323..7877
                     /locus_tag="PKY63_10630"
     CDS             7323..7877
                     /locus_tag="PKY63_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF01113.1"
                     /translation="MKKSMSELGRMSAEEFKKSEKLPLTILLDNVRSQHNIGSVFRTS
                     DAFRVSEIILCGITATPPNKEIQKTALGATDSVSWRYYENPAEAVRELKQEGFTVIAV
                     EQTHNSLLINKDHWLNEGEKIALVLGNEVFGTSDEVLELCDRTIEIPQFGTKHSLNVS
                     VCAGIVIWEICKWHLQDKKNILSK"
     gene            7983..9737
                     /locus_tag="PKY63_10635"
     CDS             7983..9737
                     /locus_tag="PKY63_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01114.1"
                     /translation="MKNLNLKIVVALVLSAALMSGCGLSKMAKKYSTVKYEAKPEMLE
                     THGGKINTAVKGVFPAKYFDKKVTLEFTPVLKWEGGSYTCKTIKIQGERVTGEGITIN
                     KVNGGTFDWSDVIPYKPEMNASELVVNVKASKGNKTKDFGEVKLADGVIYTSERVARD
                     EEMAIADHGYKKEVLITETANLYFDYNSANYSKTQKLNNSDASKEALKKAEEFIAKGW
                     KIKTIDINAWASPEGEEAYNAELSNDRSEVAKKYIDSYIEKLDKQIAKDKKAKYEEIK
                     RAYTINPKANGEDFEGFMAAIQKSTLPEKQAIINVIKSQPSKVQREQEIKNMTVIYAQ
                     VEEILEPLRRAEISVVCYEPKRTDENIAMLSTTAPDSLKAEELLFAATLTEDNQTKVN
                     IYKSGTKTYADNWKFWNNCGAILMEMGEVNDAKPYIEKANALSPNNAVVMNNMGVLAS
                     WDKDYATAKTRYEAAQSGGVSANYNLGILKIREGDYDGALQLFGSKTCKYNVALAQVL
                     KLDYTNATSNLNCCKLDNGAPFYLLAVIGARTNNSSMLYDNLKKAIAAEPAYKAQAKE
                     DREFFKFFNSTDFQNIVK"
BASE COUNT         3059 a         2328 c         1933 g         2591 t
ORIGIN      
        1 cagtcatttc tgtagctgct tcggatgcca atatctgtgt cggcgaaaac tctgtactga
       61 ccgctacaag cgacattccg ggcagctcat tcctttggag caccggcgcc aacaccgctt
      121 ccatcactgt ttcacctgcg gcaacaacca catacaatgt taccgttacc agccccgaag
      181 gatgcgtaag taccggcgaa gtaactgtaa atgtctttcc aaatccggtg ctgacaatta
      241 ctgcaagctc aggcgccatc tgctttggag acccctctgt catctctgtc tcttcgtcca
      301 ttgctgccac aacttttgaa tggagcacag gagaaacaac ggcaaacatt actgtttcac
      361 cagcaagcac aagcacctat tttgttaccg gaacatcggc caacggctgt accggaaccg
      421 caagcattga aatcatcgtc aatccgctgc cagtcatttc tgtagctgct tcggatgcca
      481 atatctgtgt cggcgaaaac tctgtactga ccgctacaag cgacattccg ggcagctcat
      541 tcctttggag caccggcgcc aacaccgctt ccatcactgt ttcacctgcg gcaacaacca
      601 catacaatgt taccgttacc agccccgaag gatgcgtaag taccggcgaa gtaactgtaa
      661 atgtctttcc aaatccggtg ctgacaatta ctgcaagctc aggcgccatc tgctttggag
      721 acccctctgt catctctgtc tcttcgtcca ttgctgccac aacttttgaa tggagcacag
      781 gagaaacaac ggcaaacatt actgtttcac cagcaagcac aagcacctat tttgttaccg
      841 gaacatcggc caacggctgt accggaaccg caagcattga aatcatcgtc aatccgctgc
      901 caacaatcac aacgacggta acagacagta taatctgcga tggagaaagt gttacattaa
      961 cagcattctc agatataacc ggatcgacat atcaatggag cacaggcgca tcaacaccag
     1021 aaattattgt agctccaaat ctgacaaaca cctattatct gactgttaca tccccagaag
     1081 gttgtgtatc gacagccaac agcaccatta cagtaaatcc taatccgatt gcaagcatag
     1141 taccatcatc aggcgctatt tgcatggggt caacagcaac actgaccgcc aacagcaaca
     1201 ttgcagtcaa cagctatctg tggaataccg ccgagacaaa tccatccatc attgttacac
     1261 cggctacaac aagcacttat acagttactt tgacaactgc agaaggatgt tcaggaacag
     1321 ccgacatttc ggtttttgta aatccgctgc cagatatttc ggttacagca ccagccgcag
     1381 cgatttgtat aggatcatcg cttgatttaa cggcaaacag caatcatcct tcaaccggtt
     1441 atgaatggag caccgggcaa acaacgccca gcatcaatgt atcaccagct gcaagcagca
     1501 cttattccgt aacaggaacc gacataaacg gatgcacagg agaagctgaa ttcattgtaa
     1561 ccgtaaatcc tttacccgac atcaacattg tttcggcaca aaacgaaatc tgtgaaggaa
     1621 tgagtgtaga actcacagcc accagcaaca ttcctgacac cgattttgaa tggaacacag
     1681 gacaaacaag cagctcaatt attgtcagtc caccagcgag tacaacgtac gaagtaaccg
     1741 gaaccgatgc caatggttgc acaggaagca atcgaattac cattcaggta aatccaaatc
     1801 caacggtaac gataaatccg aattcgctca ctatttgtcc gggcgacaca gcaaccctga
     1861 atgcctcatc tgccatggca attacctatg catggaatac cggagcaaca acaaccagca
     1921 tttcggtatg gccatcaaac acaagcagct acagtgtaac cggaaccgac atcaatggat
     1981 gcattggaat agccgcagcc acagttgttg tgcgcaatgc accggtcatt gtagtctcgc
     2041 cttacaatcc aaaaatctgt tatggagact ctgtcacact catagcatcc ggcgctgttt
     2101 tatacgactg gactccagcc acagggctca acacaaatgt cggacacata gttacatcat
     2161 caccagactc gacgaccaca tatatcgttg ccggagttga tttatacggt tgtcagggat
     2221 caaccaatgt tacagtaaca gttcatccga taccaactgt tgatttccgt gcagatgaaa
     2281 cagatatctg tcaggcaaca gtagtacatt tcagcggaat ggcttcccca tcgattcagt
     2341 catgggaatg ggattttggt gatcctgctt caggagccag caattcgtca tcgatgcaaa
     2401 atccgatgca tgcctttttt gaaaccggaa catataccat tggattaagt gttgttacaa
     2461 cagcaggatg caaaaactcc atacaaaaac caagttatat tattgtacat ccaaatccta
     2521 tagcaagttt tgcacgtacg ccagatataa caacactgga atatccaacc attcaattct
     2581 acgatcagtc tctgggtgaa aaatcacgac tctgggattt cgacgatccg ggaagtggtc
     2641 ttgacaacaa cagcattgca aaaaatccga cacacaccta cagtgcatca ggagaatact
     2701 gggccacatt gtttgtcgaa aatgaatggg gttgtctcga ttctgtaagc aatcgcatca
     2761 tcatcaatcc aacatgggac gaatacattc cttcagcctt ttcaccaaac ggcgacggca
     2821 acaatgatgt attccgtcca atgggattca acattgattt cacccaatac caaatgtata
     2881 tttacgaccg ttggggcaag gaaatctttg acacagacaa tattgaagaa ggatgggacg
     2941 gtcgggtcaa cgatcacggc gaaaccgttc aacaagatgt atatgtttat ctgattattc
     3001 ttacagacat cagtggtctt gaacatcagt ttatcggaca cgttactgtt gttaaataaa
     3061 cgttgttcaa ttacatggct caaaagccct ccggtttgga gggctttttt gttgaagcaa
     3121 aaatcataaa caaaaaaatg aggaatgaaa tataatgcaa caaatcagct tttcagaaaa
     3181 tcgctgacaa tatccaaggc ctcgctgaca gcagtttcaa gctcattatt cacaatcacc
     3241 acatcgaatt caggcgcaaa gcttaattca tattttgccc ggtcaaggcg tttttggatt
     3301 gcttcttcag actctgtatg tcttaatctg agtcgttctt ccaatatttc aaaagaaggt
     3361 ggctgaataa aaacagacaa ggccttttcg ccaaaaatat ttttcagatt tattccaccc
     3421 ttaacatcaa catcaaaaat aatggcgttg ccattattcc agacacgttc tacttcggac
     3481 ttcaatgttc cataaaaaag tccatcatat acttcctccc actctaaaaa attttgttgt
     3541 tccacttttt ttctaaattc ctctgcggaa aaaaagaaat aatcgatttt gtttatttca
     3601 ccggttcgtg gttttctgct ggttgccgag accgagaatc ctaatggcaa tcccgcatca
     3661 ataagtcgat gcacaatggt agttttacct gccccgctgg gcgccgaaaa aatcactgcc
     3721 tttttcatat atcagagaat attggccagt tgttctttga ttttctccag ttcatctttc
     3781 atcacaacca cacgtttctg aatatcaaag aaattgcttt tattacccag cgtatttatc
     3841 tcgcgcccca tttcctgagc gataaaacca agttttttcc cggccggctc cgaatcgttt
     3901 tgtttcagcg tgttgcgaaa atattcgata tgctttgcaa gacgaacctt ttcctcattg
     3961 atgtcaaatt tatcaatata ataaaacaat tcctgttcaa gtcttgtttc atcagttttc
     4021 acatcgagtt gtgcctgttg gagttctttt accaaacgct cacgaacctg tatttttcgc
     4081 tccggatcca gctgttctat ttcaggcaac aatttttcaa ttacatcgag atgacggatc
     4141 atatcagttt caagcacagc tccttcctgc atacgaaaag catctgtttc ttcgcatgcc
     4201 ttgcgaacca gctcgaacaa agcaagccat tcatcttctt caacaactgg atttccgtaa
     4261 ccaaccacct ccggaagccg gatcaaaatt tccatccaat tgtcgggttt attgatacca
     4321 agatgatcag acaacactga aagttcagta aaatactttt tagcaagagt tttattcaca
     4381 cttgcatcgg catttgttac atcctcaatg atcacattaa tgtcaacctt tccacgcaac
     4441 aaaatttttg cagcaatatc acgtatctga ttttcacgtt cacggtaccg aaacggcgtt
     4501 ttcaccgaca agtcaagttg cttattgttg agggttcgca ccataacagc aacggttgaa
     4561 ttaccaatgc ttgtatgcac cctgccaaaa ccggtcattg atctgagcat aagaattatt
     4621 gttttttatt tttttcgagt atctttttta tgctgttcag cttcatcagt gcttcgaccg
     4681 gtgtgagcat atcaacattt gtattcagaa tttcatcacg aagattttca agcaaaggat
     4741 catccaactg aatcagactg agttgaaaag gttccgctgc tgttttggtt ttatttgatt
     4801 caccaactga aatatccgag ccatgttctt tttcaagacg cacgagaatt tcccctgcgc
     4861 gttcaataat attacggggc acgcctgcca tgcgggctac atgaatgcca aaactatgtt
     4921 ctgttccacc tggagtcagt ttgcgaataa aaacaatatt gtttccgctt tccttaaccg
     4981 aaatatgaaa attacgcaca cgtggcagat aggttgccaa ttcattgagc tcatgataat
     5041 gtgttgcaaa aagcgttttg gcatgacaga gcggatgatt gtgcaaatat tccgtaattg
     5101 accaggcaat agaaatacca tcataggtcg aggttccacg gccaatttca tcgagcagga
     5161 taagactccg caaagaaaga ttattcagaa tactggctgt ttcattcatt tcaaccataa
     5221 aggtcgattc acccgaagtg agattatcag aggcccctac ccgggtaaaa attttatcca
     5281 ccaacccgat ttcggcagaa gccgccggga cataacaacc acattgagcc atcaggacaa
     5341 taagcgccgt ttgccgcaac agagccgatt ttccagccat gttgggtccg gttaaaataa
     5401 taatctgctg atcatcactg tttaataaaa catcattttc aacatattta tcacccggcg
     5461 gcaactgagt ctcaataaca ggatgacgcc cgcccttgat atcaatggtc aaaccatcat
     5521 tgatgactgg tctgcagtaa ttgttctcgg aagcaataag cgcaaaggaa agaaaaacat
     5581 cgatgatggc cagcaacgaa gcattttttt gcaatgctgc ggtaaaaggc attgactgct
     5641 gaatcagcga agcatataaa tcctgttcaa ttcttagaat tttttcctca gcccccagaa
     5701 ttttttcttc atatgtttta agttccggcg taatatatcg ttctgcattg gccagtgttt
     5761 gcttgcggat ccattcttca ggaaccttat ccttatgtgt attggtaact tctagataat
     5821 aaccaaacac attattgaag ccaattttca gcgatgctat tcctgtgcgc aacgcttcat
     5881 tctgctgaat accggaaaga aaatttttac tgtctgtgct aagcaaacga agttcatcaa
     5941 gctcattatt gatacccgga caaataacac caccctttac aatcaaagcg gcagcatctt
     6001 cgcggatata tttttttatc aagaccgaaa gttcatcaag attggcaaaa cgcgaaacga
     6061 ttttttgcag tgggtctttt tcgtttggaa taattgcaca aagttctttt gttttttcaa
     6121 tggaatatgc aagcgtaagg aattcgcgcg gattaatttt tccaacggca agtttgccac
     6181 aaagccgctc caaatcagct atttcagtca ataattgttg aacctgaaaa gacaattcct
     6241 tattttcgta aaaacaggaa actgcatcaa ggcggtcatt aattttatct ttattaatca
     6301 gagggagcaa caaccaacgc tgcaacaagc ggctacccat gggcgaagaa gtccggtcga
     6361 tagtactcag taatgtccgg ccacgctcat tgtatgactg caccagttca agatttcgga
     6421 ttgtaaaacg gtcgagccac acataatcat cattatcaat gcgtgatacc gatcggatat
     6481 gagcagagcc accgttctga cgtgtttcat tgatataatg gaggcaagct ccagctgcaa
     6541 tgacagcatt ttccatggta tcgacaccaa atcctttcag attttctgtt tcgaattgcc
     6601 ggagaagtaa atcctgtgca tattgagcag aaaatatcca atcatcgaaa gcaaacacat
     6661 tgaacggacc aaaaacatca agaaattcat ttttctgtgt ttttttaaca agcacttcag
     6721 acggttgcat gttctgaagc aagcgggcaa tatattcatt acttccttcg gccacaaaaa
     6781 attcaccggt tgaaacatca ataaaagaag ctccggtttt gggagcatgc agaaaaacgg
     6841 aagccaggaa attattactt tttcggtcga gcacattgtc ggaaaatgcg acacccggcg
     6901 taacaagttc ggtgataccc cgtttgacaa ttttttttgt cagtttaggg tcttcaagtt
     6961 gctcgcaaac agccactctc tgcccggctt taacaagttt gggcagatag gtttcgaggg
     7021 catgatgagg gaatccggcg agttcgatat gcgaagccgc gccattggct cttttggtaa
     7081 gcgtaatacc taaaatagcc gacgccctta cagcatcttc gccaaaggtt tcaaaaaagt
     7141 cgccaaccct aaacaacaga atagcatcgg ggtatttgac tttaatttca ttgtattgcc
     7201 tcatcagagg cgtttcagca acagatccta cgctcacttg attcagtctt tgcaacaaaa
     7261 atatgaaatt ctttttaacc atgtttgtat ttttgtctcc aaaacaaaga agttaacagc
     7321 ttatgaagaa gagcatgtcg gaactaggaa gaatgtcggc cgaagaattc aaaaaatcgg
     7381 aaaaactgcc cttaaccata cttttggaca atgtccgcag tcagcataat attgggtcgg
     7441 tttttcgcac atccgatgca ttcagggtat cggaaattat tttgtgtgga atcacagcaa
     7501 ctccgcccaa caaagaaatt cagaaaacgg cattgggagc gaccgacagt gtatcctggc
     7561 gttattatga aaacccggcc gaagctgtca gagagttaaa acaagagggt tttacagtaa
     7621 ttgccgttga acagacccac aacagtttgt taatcaacaa agaccattgg ctaaatgagg
     7681 gcgaaaaaat tgcacttgta ttgggcaacg aagtatttgg cacgtcggac gaagtgcttg
     7741 aattatgcga ccggaccata gagattccgc aatttggcac aaaacattca ctgaacgtat
     7801 cagtatgtgc cggcattgta atttgggaga tctgcaaatg gcatctgcag gacaaaaaga
     7861 acattttaag caaataacat tttaacatat tatgcggcaa actgaaaaaa atattatata
     7921 tttgcccaaa attcctattt ttgcagttca acaaaaaacg aaaccaataa acaaccaatc
     7981 ttatgaaaaa ccttaatctg aaaattgttg ttgctcttgt actttcagca gcgctcatgt
     8041 caggttgcgg tctcagtaaa atggccaaga aatacagcac ggtcaaatat gaggcaaaac
     8101 ctgaaatgct ggaaacccat ggcggtaaaa taaatactgc tgtaaaaggt gtattcccgg
     8161 cgaaatactt tgacaagaaa gtcaccctcg aattcacccc ggttctgaaa tgggaaggtg
     8221 gcagctacac ctgcaagacc atcaaaattc agggcgaaag agttaccggc gaaggcatta
     8281 ccatcaacaa agtaaatggt ggcacatttg actggtctga cgttattcct tacaaaccgg
     8341 aaatgaatgc ttcggaactt gtggttaatg ttaaagccag caaaggaaac aaaacaaaag
     8401 atttcggcga agttaaactg gccgatggcg ttatttacac cagcgaacgc gttgcccgcg
     8461 acgaagaaat ggccattgct gatcacggct acaaaaaaga agtcctgatt actgaaactg
     8521 ccaatcttta ttttgattac aacagcgcca attacagcaa aacccagaaa ctcaacaatt
     8581 cagatgccag caaagaagcg ctgaaaaaag ctgaagagtt tattgccaaa ggttggaaaa
     8641 tcaaaaccat cgacatcaat gcatgggcat ctcctgaagg agaagaagct tacaacgcag
     8701 aactttcaaa cgatcgttca gaagttgcca agaaatatat tgattcatac attgagaaac
     8761 ttgacaaaca gattgccaaa gacaaaaaag ccaaatacga agaaatcaaa cgtgcttaca
     8821 ccattaatcc aaaagccaat ggtgaagatt ttgaaggttt catggctgcc attcagaaat
     8881 caacccttcc tgaaaaacag gccatcatca atgtgattaa atcacagcca agcaaagttc
     8941 agcgcgaaca ggaaatcaaa aacatgaccg ttatttatgc tcaggtggaa gaaattcttg
     9001 aacctctgcg tcgtgccgaa atttcagtcg tttgctacga accaaaacgc accgatgaaa
     9061 acatcgccat gctttcaacc acagctcctg acagcttgaa agctgaagaa cttcttttcg
     9121 ctgcaactct taccgaagac aatcagacca aagtaaacat ttacaaatca ggtacaaaaa
     9181 catacgctga caactggaaa ttctggaaca actgcggtgc catcctgatg gaaatgggcg
     9241 aagtcaacga tgccaaacct tacattgaaa aagcaaacgc tctttcacca aacaacgctg
     9301 ttgttatgaa caacatgggt gttcttgctt catgggacaa agattatgca actgccaaaa
     9361 cacgttacga agctgcacaa agtggcggtg tcagcgctaa ctacaacctg ggcatcctca
     9421 aaatccgtga aggtgactat gacggagctc tccagttgtt tggcagcaaa acctgcaaat
     9481 acaatgtagc tcttgctcag gttctcaaac ttgactatac caacgctaca tctaacctca
     9541 actgctgcaa acttgacaac ggtgctcctt tctatctcct cgctgttatc ggcgcccgca
     9601 ccaacaactc aagcatgctt tatgacaatc tgaagaaagc tatcgctgcc gaacccgctt
     9661 acaaagctca ggcaaaagaa gaccgcgaat tcttcaaatt cttcaactca accgatttcc
     9721 agaatatcgt aaaataatat tcgggattcc aatactgaag gggctttcca aacggaaggc
     9781 ctcttttttt gtgccctgca taataagcca caaagacaga aagacaccat tatttagggc
     9841 ctgctgaaaa agtcttaggt gcgccatacc gtaacttcgt acggcacaag tattcgaggc
     9901 gacaagacga a
//
LOCUS       DAOPKE010000098         9748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7302_length_9748_cov_11.535954, whole genome shotgun sequence.
ACCESSION   DAOPKE010000098 DAOPKE010000000
VERSION     DAOPKE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7302_length_9748_cov_11.535954"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKY63_10640"
     CDS             complement(<1..224)
                     /locus_tag="PKY63_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01115.1"
                     /translation="MSNNNSIRLSCHVLREAGCKEAQKQDPPAGGSNPPVFEKHCFNI
                     FVLFDLSEVVFCLSFTRPFHVKRDTAGRAI"
     gene            complement(613..873)
                     /gene="rpmA"
                     /locus_tag="PKY63_10645"
     CDS             complement(613..873)
                     /gene="rpmA"
                     /locus_tag="PKY63_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPF01116.1"
                     /translation="MAHKKGVGSSSNGRESHSKRLGVKIYGGQIAKAGNILVRQRGTK
                     HNPGENVGMGKDHTLFALKDGIVEFRKTRDDKSFVTVKEQQN"
     gene            complement(1156..1461)
                     /gene="rplU"
                     /locus_tag="PKY63_10650"
                     /pseudo
     CDS             complement(1156..1461)
                     /gene="rplU"
                     /locus_tag="PKY63_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            1714..3033
                     /locus_tag="PKY63_10655"
     CDS             1714..3033
                     /locus_tag="PKY63_10655"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01117.1"
                     /translation="MKSQTISALIVLAILILFSASATAQCPATKYGLNPVWPVNWTQT
                     DRQNWYLDMAVHGQGFQSDGRTWRQLQVMMDSNTLVPFRDEIMWAKTNGGIEKYLFQF
                     KNPVSVSNQMPPVWCGNPLTDTNTTNALYSFTTAFLDTMHQVLDYFVLGSETDIYFKS
                     GPAERDSFFVMAQRISDYIDLHYPEIRFGIGVSMFCALNPDNHYWNMVLTRGDIMSVS
                     WWPLDSYYMADTSEINSADSVISVLLAKCGSKPVVISDCGVTTAMAPERDDIQSEFVR
                     NTFLYTMNVPQIEAVGYYYLADFDTTAIYYQQNLYMTYAPEFHQSIKSRGLLDSLGNP
                     KPSFDVYISMLDTVCAFVGNEEQQLNSEISIWPNPASEKLFVNGNGFSFYRIYATSGQ
                     LMAQGLLNKNGSPEIDIARLADGAYILYLISEKKSPENILFIKERQP"
     gene            3030..3770
                     /locus_tag="PKY63_10660"
     CDS             3030..3770
                     /locus_tag="PKY63_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPF01118.1"
                     /translation="MKAMIFAAGKGTRLRPITDTVPKAMVIVGGKPLLEWLLLKMAAS
                     GIRDVVVNVHYLSEQIIDFINRKPVQGLNIQISDERNLLLDTGGGLKKAAPMLQGNEP
                     ILIHNVDILSNLDFGQFEKDFLKAATPAMLAVKDRQTTRKLEFSDEGLLRGWINESTG
                     EHISVNDFQGQVRRFAFSGISVVSAEFPLLMRESGVFGFIPACLTLARNIKIKKYDAS
                     HEWLDVGKPEVLQKAEQFVYSYYNAFTK"
     gene            3767..6592
                     /locus_tag="PKY63_10665"
     CDS             3767..6592
                     /locus_tag="PKY63_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPF01119.1"
                     /translation="MSFIQETAKKIIESCGTDLRSVAIVVPGRRGGLFLKKELAMAAG
                     KTILLPQIFSIEEYIFDQSGMRKMDETTMLTLLYTVYKKHAGQSAFPIDEFLPTGKTL
                     LADFNDVDDYLLDPDEVFTFLMRVREIENWMPGEAETELQKRYLSFYQLLKPMYHLFT
                     EKCHETGMCYQGKASRILSQQDTFSDFSRIVFAGLNAVTPSLEVHINKLLLSDQALLI
                     WDADSWYLDNPNHEAGHFMRLNRNSWPKTFGETPRFFENISKKIEFIGAPLGYSQVQS
                     ALTLLDTKYRDDISNTLLVLADEGLMMPLLSSLPEEWDDRVNISGGYPLRNTLLGQFV
                     NAQLELISGTLNENGAVRTYRYEQLLEVVSNPLLRLLPDENQKREFSEVVDEISSGRL
                     RYFRWCESDKDDTLLLKRFNVLIPGVVSPCESWDDVLSRLISLTSELFEKCREVDLEH
                     EAAWLVQSMLKALSNTLKNYGDVSDVTIGSVVYFIRRHLQIAKIPLKGEPLKGIQILG
                     MLETRSLDFKNVIIVGANEGFLPAGGQGDSFLPNDVRRELKLPLQYERDAVYAYHFWR
                     LIQRAEEITLIYNTESDPLFGKEPSRFLAQIEHELKHVFPSIVVERRVLGISYDKQPQ
                     TSFNFVDVNKIQVVLNKIAESGLSFSRLYNFIACPHRFLLESIYNIQKWEDETGDIAI
                     NTMGSVFHDAIEHLTIPFLGKILVKQDFDTMSAAAETALHDAFQHLKVSHTTSGYNFL
                     VKEVLSDTLLHWLHKMKQTLPGDYYVRAVEQSLKPQISLKDGRIVTLKGVADRVEFCN
                     GIMRVMDFKTTNKRSRFIFDDGPDAGIYEQICHPENHYMTQVLFYSWLFSLTENVEAI
                     TGGVYPVLNSAGYNPDLLRSKDKKLLVLKADQLKEIGGVFFDFIEQIFDPQQTFDPQP
                     AVKTCQYCPYNGVICFANTFEITEDES"
     gene            6582..7547
                     /locus_tag="PKY63_10670"
     CDS             6582..7547
                     /locus_tag="PKY63_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01120.1"
                     /translation="MNHRKSLLIFLLFLAVSFSSFGQQPHGIFWSPELRLNPALTGVM
                     TGKWRLMEHHRSQNFNQGGTLHWNTISADFRKDIIKKSGGYGLVMDEATGNTFGFGIM
                     DDRRRSDSTGAEYIADYFSFSYRHAFKKSTFGFGIQPGYMRSEAEKKFDLNAGLLWGS
                     GTIYCWQEDQIFKNQIGIAAYNILSDWADTALMPGREVQLHAGFLIKKVEQFNFVVNG
                     LYRYNGSSDFAFGTYAIFFPIVHYAYWDRARLGLHYRSSNHLTWSAGLRFYGRGRKTF
                     TLDGTISYDMPMKFLDFESKYKNAWEIGIVITRFEKCWSTDKCGN"
     gene            7839..9092
                     /locus_tag="PKY63_10675"
     CDS             7839..9092
                     /locus_tag="PKY63_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPF01121.1"
                     /translation="MKYIERITDTELKRKLKASGAVLIRGAKACGKTESAKQFAKSSL
                     NVDRDEQVSVLIDAAPKRLLIGETPRLIDEWQVQPKLWDYVRHEVDARNKSAQFILTG
                     SANPVETVKMHSGAGRFTIVDMRTMTWQELGYSSGKISVANLLKGEKMSVLDSPTDLE
                     FIIEKIVIGGFPTLLNKRINQATDLNRAYVELLAEVDMSRVSNVNRDPVKVMSLLRSL
                     ARNTATLVEYCALERDIRENDNIGISRPTIYEYLNVLNRLMIVEDQPAWNTHIRSSYS
                     LRKTPKRHFTDVSLAVAALGADKNALLNDLNFTGFLFESLATHELRVYAQANDAKVYH
                     YRDSSGLEVDCIVQKFNGDWCAFEIKLGTGQIDDAAATLNKFASILDTKKCKPPKSLN
                     IITGTGISYTRKDGINVISLASLGR"
BASE COUNT         2747 a         1814 c         2215 g         2972 t
ORIGIN      
        1 ataatagccc ggcctgctgt gtcacgtttt acgtgaaatg gccgggtaaa agataaacaa
       61 aaaacgacct cggagaggtc gaataaaacg aaaatattga aacaatgttt ttcaaaaaca
      121 ggtggattgg atccgccagc tggcggatcc tgtttttgtg cttctttaca tccggcttca
      181 cgtaaaacgt gacaagatag ccggatgcta ttattgtttg acatcctccg gacgtcaata
      241 ttattcaata ataaaacaca atgtcttttt tgccgggaca cccatttgag caaccttaaa
      301 attgtataaa tgtatatatc aattgcttac acaattggaa atttcaaagt gccgaagtca
      361 ggagtaatga actctgaaca gcgaacaagg aagtgcgaag tttgaagtaa aatccacttt
      421 cttgttcttt tttatttttt attgatctgt cagagtgcgc gaattgaatt tgggaatttt
      481 tcctgtaatg gaggaatgat tctgatttcg tgtctttgtg ccctagtggc aatttttccg
      541 gtatctatcc gataacaaaa aaaaaccacc tccgcagccg aaggtggttc acagtaactt
      601 tattcagatt aattaattct gctgttcttt tacagtgaca aaagatttat cgtctctggt
      661 tttgcggaat tcaacaatac cgtctttcag tgcaaaaaga gtatggtctt tacccatacc
      721 gacattttcg ccgggattgt gtttggtacc gcgctgacga acgaggatat tgccggcttt
      781 ggcaatctgt ccgccgtata tttttacacc aagtcgcttg ctatgtgatt cacgaccgtt
      841 tgatgaacta ccgacacctt ttttatgagc catatctaaa gattttaagc gttaactgat
      901 tgagaattat tcgtccttct tggttttctt ggcagccggt tttttagcgg cgggcttttt
      961 ctctgcagcc ggtttctttt ctgcagcggg ttttttagcg gcagcttttt tgggagcggc
     1021 ttttttcacc ggagcttctt ctttttcagc tttgggttca gcaatagctt ttaccttttt
     1081 cgctttaaca ggagcttcga ctactttttt ctcaacagaa ggagcattgt cgccggtcag
     1141 gctgacaccg tctttcacga tcgaaagaat gttcaggcgg gtcatatatt gacggtgacc
     1201 gttcattttc tgatagcttt tacgacgttt cttgtgaaaa acaaggactt tgtcgctttt
     1261 catgtgctcg accacttcgg ccagaacatg ggcgccatca acattcggtt ttccaacctg
     1321 aacgtttttt tcgtcgtcga tcagtaatac atttttcagt tcaatctgac tgccttcttc
     1381 ggcctgcagg cgatgaacat acacttttcc ggctttgctg accttaaact gctgtccggc
     1441 tatatccaca attgcgtaca tcgtaagtga tttcagttgt taatcaatac tttttgaaac
     1501 gggagtgcaa agatataaac tttttttgaa acacaaaccc caatcggaaa aatatttatt
     1561 cagaggtaat tgcatctccg ctgaaaattt cggtttggtc tgttattctg tcttttaggc
     1621 tcattttcaa tatggtttgt tcatttcttc gttgttgcct gttttgtctg ttctgaaact
     1681 tattgctatc tttaatgact taaaatagta tttgtgaaga gtcagacaat cagcgcttta
     1741 atagttttgg ccatccttat tttgttttct gcgtcagcaa cagcgcagtg cccggctaca
     1801 aaatacgggt tgaatcccgt ctggccggtt aactggactc agaccgaccg acagaactgg
     1861 tatctcgaca tggctgtgca cgggcagggc ttccagagcg acgggcgtac ctggcgtcag
     1921 cttcaggtga tgatggacag caacacactc gttcctttca gggatgaaat tatgtgggca
     1981 aaaaccaatg gtggaattga aaaatatttg tttcaattca aaaatccggt atctgtttca
     2041 aatcagatgc cgcctgtttg gtgcggcaat ccgttgacag ataccaatac aaccaatgct
     2101 ttatatagtt ttaccactgc ttttctcgac accatgcatc aggtgctgga ttattttgtt
     2161 ttaggtagtg agaccgatat ttatttcaaa agcgggccgg ctgagcggga ttcttttttt
     2221 gttatggctc agagaatttc agattacatt gatttgcatt atcccgaaat aagatttggc
     2281 ataggagtga gtatgttttg tgcattgaat cctgacaatc attattggaa tatggtactg
     2341 acgaggggcg atattatgtc ggtttcgtgg tggccgcttg acagttatta tatggctgat
     2401 accagtgaaa taaattctgc agattctgtt ataagcgttt tgctggccaa atgcggaagt
     2461 aaacctgtgg ttatttcgga ttgcggggta acaacggcca tggcgccgga gcgcgatgat
     2521 attcagtcgg aatttgtccg aaatactttt ttatatacca tgaatgttcc gcagattgaa
     2581 gctgttgggt attattattt ggctgatttc gatacaaccg ccatttatta tcaacaaaat
     2641 ctgtatatga cttatgcgcc ggagtttcat caaagcatta aatcgcgcgg attgcttgat
     2701 tcgcttggaa atccgaaacc gtcattcgac gtttatatat caatgcttga cacagtttgt
     2761 gcttttgtcg gaaatgaaga gcagcagctg aattcagaaa tcagtatctg gccgaacccg
     2821 gcttcggaaa agctttttgt aaacgggaat ggtttcagtt tttatagaat ttatgcgact
     2881 tccggacaat tgatggctca gggcttattg aataaaaacg gaagtcctga aatcgacata
     2941 gcccgattgg cagatggtgc ctacatttta tatttgattt cagaaaaaaa atcacctgaa
     3001 aatattttat tcatcaaaga acgacaacca tgaaggccat gatatttgca gcaggaaaag
     3061 gaacgcgtct gcggccaatc acagatacgg ttccaaaggc catggtgatt gttggcggaa
     3121 aaccgcttct tgaatggctt ttgcttaaaa tggccgcaag cggaatccgc gatgtggtgg
     3181 taaatgtcca ttatctgtcg gaacagatca ttgatttcat caaccgcaaa cctgttcagg
     3241 gactgaatat tcagatttca gatgaaagga atctgttgct cgataccgga ggtggactca
     3301 aaaaagctgc gccgatgctg caaggcaatg agcccattct tattcacaat gtcgatattt
     3361 tgagcaatct cgatttcgga cagtttgaga aagattttct gaaagctgcg acgcccgcca
     3421 tgcttgctgt gaaagaccgg caaaccacaa gaaaacttga attttctgat gaaggtcttt
     3481 tgcgtggatg gataaatgaa agtaccggag aacatatcag tgtcaatgat tttcaggggc
     3541 aggtgaggcg ttttgcattt tcagggatat cagtagtatc tgccgaattt ccgcttctga
     3601 tgcgcgaaag cggtgttttt ggctttattc cggcatgttt aaccctagcc agaaacatta
     3661 aaattaaaaa atacgatgct tcacacgagt ggctcgacgt ggggaaaccc gaagtgttgc
     3721 aaaaagcaga gcagttcgta tattcatatt ataatgcatt tacaaaatga gttttattca
     3781 ggaaaccgca aaaaaaatca ttgaaagctg cggaacggat cttcgttcgg tggctattgt
     3841 tgtgccaggc agacgtggcg gactgttttt gaaaaaggaa cttgccatgg ctgcaggtaa
     3901 aactatttta ctgccgcaga tattcagcat cgaggaatac attttcgatc agagcggcat
     3961 gcgtaaaatg gacgagacca ccatgttgac tcttttgtac acggtttata aaaaacatgc
     4021 cggacaaagc gcttttccga tagatgaatt tctgccgaca ggcaaaacat tgctggccga
     4081 tttcaatgat gtggatgact atctgctgga tcccgacgag gtttttactt ttctgatgcg
     4141 tgttcgtgaa atcgaaaact ggatgccggg tgaagctgaa acagaacttc agaaaaggta
     4201 tctgagtttc tatcagttgc tcaagccgat gtatcatttg tttactgaaa aatgtcatga
     4261 aactggcatg tgctatcagg gtaaggcctc gcgtatttta tcgcagcaag atactttttc
     4321 tgatttttcg cgcattgtct ttgccggact caatgcggtg actccatcgc tcgaagtaca
     4381 tatcaacaaa ctcctgttga gcgatcaggc ccttttgatc tgggatgccg acagttggta
     4441 tctcgataat ccaaatcatg aggcagggca ttttatgcgt ctgaacagaa attcctggcc
     4501 taaaactttt ggagaaacac ctcgtttttt tgaaaatatc agtaagaaaa ttgaatttat
     4561 cggtgctcct ctgggttact cacaggtgca gtcagcactt actttgctcg ataccaaata
     4621 tcgcgatgat ataagcaata cgttattggt gctggccgat gagggattga tgatgccgtt
     4681 gctcagtagt ttgcccgaag aatgggatga ccgggtcaat atttcagggg ggtatccttt
     4741 gagaaatacg ttgctcggac aatttgtaaa tgcgcagctg gaattgattt caggaacatt
     4801 gaacgagaat ggtgcagtca gaacatatcg ctatgagcag cttctggaag tggtttccaa
     4861 tcctcttttg cggctgctgc ctgatgaaaa tcagaagaga gaattttctg aagtcgttga
     4921 tgagattagc tccggtcggc taagatattt tcgctggtgt gagagtgata aggatgatac
     4981 gcttttatta aagcgtttca atgtgctgat tccgggtgtt gtttcgcctt gtgaaagctg
     5041 ggacgatgtc ttgtcgcggc tgatcagtct gacaagcgaa ttgtttgaaa aatgccgtga
     5101 ggtcgatttg gagcatgaag ctgcatggct tgtacagtca atgttgaaag cactttcaaa
     5161 cacactgaaa aattacggcg atgtatcgga tgttaccatc gggtctgtgg tttatttcat
     5221 tcgccgccac ttgcagattg ccaagattcc attgaaaggt gagccactca aaggaattca
     5281 gattcttggt atgcttgaaa caagatcact ggattttaaa aatgtcatta ttgttggcgc
     5341 caacgaaggg tttttgccgg ccggcgggca aggtgattca tttctcccaa atgatgtccg
     5401 acgtgaatta aagcttccat tgcaatatga gcgtgatgcc gtatatgctt atcacttctg
     5461 gcgtttgata caacgggctg aagaaataac tttgatttat aatacagaaa gtgatccttt
     5521 gtttgggaaa gagcccagcc gttttctggc acagattgaa catgaattaa agcatgtttt
     5581 tccatcaatt gtggttgaaa gaagggttct tggaattagt tatgataagc agccgcagac
     5641 ttcattcaat tttgttgatg ttaataaaat tcaggttgtg ctcaataaaa ttgcagaatc
     5701 cggcttatcc ttttccagac tatataattt tatagcttgt ccgcatcgtt ttttactgga
     5761 aagtatttac aatattcaga aatgggaaga tgagaccggt gatattgcca tcaatacgat
     5821 gggcagtgtg tttcatgatg ctattgagca cctgacaatt ccttttcttg gaaaaattct
     5881 tgtgaaacag gatttcgata ccatgtcggc agcggcagaa acggctcttc atgatgcttt
     5941 tcagcatcta aaggtttctc atactaccag tggatataat tttctagtaa aggaagtttt
     6001 gtccgatact ttgcttcatt ggttgcataa aatgaaacaa acattgcccg gggactatta
     6061 tgtgagagcc gtagagcaat cgttgaaacc tcagattagt ttaaaagatg gtagaatagt
     6121 aaccttgaag ggtgttgcag accgggtgga attttgcaat gggattatgc gagtgatgga
     6181 ttttaaaact accaacaagc gaagtcgttt tatttttgat gatgggcctg atgccggcat
     6241 ttatgaacag atctgtcatc cggagaatca ttatatgact caggtattgt tttattcgtg
     6301 gcttttttcg ttgacagaaa atgttgaagc tataactgga ggtgtttatc ctgtattgaa
     6361 cagtgccggt tataatcctg atttgctcag gtcgaaggat aagaaattgc tcgttctcaa
     6421 agcagatcag ttgaaagaaa tcgggggggt attctttgat tttattgaac agatatttga
     6481 tcctcaacaa acttttgatc ctcaacctgc tgtaaaaacc tgccaatatt gtccgtataa
     6541 cggcgttatt tgttttgcaa atacatttga aatcacagaa gatgaatcat aggaaatcat
     6601 tgcttatttt cctgctgttt cttgcagtga gtttcagcag tttcggacag cagccgcatg
     6661 gcattttctg gtcacccgaa ttgaggttga atccggcttt gacgggtgta atgactggaa
     6721 aatggcgttt aatggaacat cataggagtc agaatttcaa ccaagggggc accttgcatt
     6781 ggaatactat ttcggcagat ttcagaaagg acattattaa aaaaagtggc ggctacgggc
     6841 ttgtgatgga tgaagccacc ggcaatactt ttggttttgg tatcatggat gaccggcgac
     6901 gcagcgatag taccggagct gaatacattg ctgattattt ttccttttcg taccggcatg
     6961 catttaaaaa gagcacattc ggattcggga ttcagcccgg atatatgcga agcgaagcag
     7021 aaaagaaatt tgatcttaat gcaggattgc tttggggcag tggtaccatt tactgttggc
     7081 aggaagatca gatttttaaa aatcagattg gtattgcggc ctataatatt ttgagcgatt
     7141 gggccgatac ggcattgatg cctggtcgcg aagttcagct gcatgctggt tttctcatca
     7201 agaaggttga gcagtttaac tttgttgtga atggattgta tcgttataat ggttccagtg
     7261 attttgcttt cggaacttat gcgatttttt tcccaattgt tcattatgca tattgggaca
     7321 gggctcgtct tggtttgcac tataggtcaa gcaatcatct gacctggtcg gcggggctcc
     7381 gtttttacgg aagaggaaga aaaacgttca cacttgatgg caccatcagt tatgacatgc
     7441 ccatgaaatt tcttgatttc gaaagcaaat ataaaaacgc atgggagata gggattgtga
     7501 ttacccgatt cgaaaaatgc tggagcactg ataagtgcgg aaattaggaa cagcgtgttg
     7561 atatgagccg aaggcaattg tgtagtttat gtcagcattc tgctggcgta atagctcatt
     7621 ctcaattcta attgcatttt cgcatattct gttctttgac cctgttttca gaaataaatt
     7681 tgggagcttt ttcctggtat ttccaaatta tgtaaagtaa aatggtcaaa atatgtaaag
     7741 taaaaatgta taaatatgta aagtaaaata attaagaaat gtaaaataaa atgatcaata
     7801 tatgtaaaat agtattacat ttgcattaat tatttcccat gaaatatata gaacgaataa
     7861 cggatactga gctgaaacgt aagctaaaag cttcgggcgc agtattgata cgaggtgcaa
     7921 aagcatgtgg aaaaactgaa tcagcaaagc aatttgctaa aagctcactt aatgtcgaca
     7981 gggacgaaca ggtttcggtt cttatcgacg ccgctccaaa aagactgttg attggagaaa
     8041 cgcctcgatt gattgacgaa tggcaagtac aacccaagct atgggattat gtacggcatg
     8101 aagtagatgc tagaaacaaa agtgcacaat tcatcctgac cggatctgca aaccctgtag
     8161 aaacagtaaa aatgcattcc ggggcaggtc gttttaccat tgttgatatg cgcacaatga
     8221 cgtggcagga gttgggttat tcatctggca aaataagcgt tgccaatctt ctgaagggcg
     8281 agaagatgag tgtgttggat agccctactg atcttgaatt cattattgag aaaatagtta
     8341 tcggcggatt cccgacacta ttaaacaaga gaattaatca ggcgacagac ttaaaccgtg
     8401 cttatgtgga gttgctggca gaagttgata tgagtcgggt ttccaatgtc aatcgtgatc
     8461 cggtaaaagt gatgagtttg ctgcgctctc ttgcccgaaa cacagccaca ttagttgagt
     8521 attgtgcatt ggaaagagat atccgggaaa acgacaatat tggtatttcg cgacctacaa
     8581 tatatgaata tctcaacgtt ctcaatcgtt taatgattgt ggaagaccaa cctgcatgga
     8641 atacgcatat tcgttcatct tattctttgc gaaaaacacc caagcggcat ttcactgatg
     8701 tttctttagc tgttgctgca ttgggcgctg ataaaaatgc gcttttaaac gatctgaatt
     8761 ttaccggctt cctgtttgaa tcactggcta cgcacgaatt gcgggtatat gcacaggcaa
     8821 acgatgccaa ggtctatcat taccgtgatt ccagcggttt ggaggttgat tgtattgtac
     8881 agaaatttaa tggtgactgg tgcgcatttg aaatcaaact tggtaccgga caaattgacg
     8941 acgctgctgc aactttaaat aaatttgcat caattctgga tacaaaaaaa tgtaaaccac
     9001 caaaatccct caacattatt actgggacag gaataagcta cacccgtaaa gatggtataa
     9061 acgtaatttc attggcatca ttaggccgat aataactcaa aaaattccgg gcaggaaaat
     9121 ctgcaatgca gtcttgactc cattcctaca gtagaggcaa aaaaacagtc acatttgatg
     9181 gcaccatcag ttatgacatg cccatgaaat ttcttgattt cgaaagcaaa tacaaaaatg
     9241 cctgggaaat agagattgtg attacgcgat tcgaaaaatg ctggagcact gataagtgcg
     9301 gaaattaggg ctttcgtgct gaaatgaccc gaaggcagtt gtcgtacatt gtatccttgc
     9361 ggctacatcc tgaaccagat caaataatcg gattcaggat tactttttct aagttattta
     9421 tgtaaacatc tgttggaatc agggatgaat cttttgtaca tttacatata ccaacttcgg
     9481 taatataaag cgaccatgta ttttcctgtg tgtttaaata gattttcgaa tgattatttg
     9541 ctcatgcaat agtctgctca aagctgccgg ttcaacaatg atattattga aggcggctgc
     9601 agacacaaca gcatgaagcg gaagctcccg gacacaaaat ccgggagctt ttttttgaat
     9661 atgcagatcg aatcatgatt gattcctgat atttttaaat agggtctgct gaaaaacacg
     9721 taacactcac aaaaacagaa atatattc
//
LOCUS       DAOPKE010000099         9443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7630_length_9443_cov_12.164252, whole genome shotgun sequence.
ACCESSION   DAOPKE010000099 DAOPKE010000000
VERSION     DAOPKE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7630_length_9443_cov_12.164252"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1751
                     /locus_tag="PKY63_10680"
     CDS             216..1751
                     /locus_tag="PKY63_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPF01122.1"
                     /translation="MRFSFFLLIAFLCTYAYAQQQTPKNIIILIADGGGYNQMMCTDF
                     FMYGDYPKSPLAAFPVQLAMSTYNGSLHSHGATCYDSKKFWTDFSYGMAGFTESAASA
                     TAMGTGFKTHNSMIGMNLDGNPMTNVIEIGKSKGKKTGVVTSVPFCHATPAGFSTHSK
                     SRHGFSEITGQMLNSGILDVIGGSGHPYFDNDGNAVQKPFTKYIDTASWKNLKAGKGN
                     IKWFYTESRSEIQTLADRKTAPEKLFMLAPVSGNLAEARDGHSIIPFDIPVKQSIPTL
                     AEMSLAALNTLSTDPDGFILMIEGGAIDWADHDNNMPRMIEEYMYFYNTVDSVQKWLK
                     EKGLLEETLIIVTNDHECGYLWGPGCAGSTFSLPQNNGKGNLPTFKYYSDNHSNTLVP
                     FFAKGPSSEIFYALADENDSVRGPYLTNSEIAIAVKMLWGNAVEIYESSSSKKDSLTL
                     FVTLPCADASIQWKANNTDISGANTVQFFIDPNVYTKPLSISCEVTCGQTTFTTQEYE
                     YKP"
     gene            1735..2283
                     /locus_tag="PKY63_10685"
     CDS             1735..2283
                     /locus_tag="PKY63_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01123.1"
                     /translation="MSTNLKQFCFLLFAITTLSISAQNDDIGSGLKNHLFTGGALGLT
                     FGNETNIEAAPLFGYHLSNVVSFGIGGTYQYYHSRYFNESMNIFGGRAFLRIQPLKPV
                     FLHAEYQFLTYKTNIFNAPTYQVQQIVAEGLLVGAGYREYLSDRLSTVIMLLYDLNYT
                     KYSPYSNPLFRFGIEYAIPLKD"
     gene            2781..3524
                     /locus_tag="PKY63_10690"
     CDS             2781..3524
                     /locus_tag="PKY63_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF01124.1"
                     /translation="MKTQILSLAIMLVLSIASISPVFSQAGGTIKGTVIENISGNPIP
                     FVSVFVKYGDNLIGAQSDFDGNFTIKPVPPGTYSVTFKCVGYGDKIVTDVVITQDKYT
                     YLNEVTMSAGIDIPVVVIPANKMIDPELTSLKVIDQTFIESVPNYGNINKLVAISSSD
                     IQVSDDDSEIYFRGSRSGDALYIIDGMRMINPGALCPSLAIGSMSVYTGGVPAKYGDF
                     TGGVIVIETQSYFEWLAAQESERLRNGGF"
     gene            3535..4101
                     /locus_tag="PKY63_10695"
     CDS             3535..4101
                     /locus_tag="PKY63_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF01125.1"
                     /translation="MQFLLNRTNKNLADEELVSRFAENGNPSIIGILFDRYAKLVMGV
                     CLKYLKDEDEAADTTMTIFKNLLETLGNYDVRAFKPWLYTYAKNECLMKLRKAKPVVC
                     SIDKVAFGLAGEEDDEERIDLPIEQLQSAIAALKNDQQVCIQMFFLENLSYNEISDKT
                     GYSWKEIKSHIQNGKRNLRNILISTGNE"
     gene            4094..5215
                     /locus_tag="PKY63_10700"
     CDS             4094..5215
                     /locus_tag="PKY63_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01126.1"
                     /translation="MNNKTYISPPEQEDDPFLRDAQEGWTQFPGARSRWWKKKLQFNL
                     FLFGKSWSVLPAAGKLAIGTLSSVALITVAAVTIPFNFSSNKQELAQQETTSQKDKII
                     PSEEIITNENVSQEETKTEGFTTITNEEQNSNHTIIQNNIPVAEDETEQATVYDWRDY
                     DMETDKISETEKYTESNARMHPVQAKKVNNEISGGLKNALPYGYISQYRYLDYDALNN
                     DIPQAPTSVTTGSLDAKFSNHLEKEKVEASVVLDTIPYKTFVQDAVTKIKDGKYAAAE
                     YQFKKLLVQRPTDENAIFYLGYCFFQQGNYPLALSYFEKTKKTKYMAFMSEADWYTAH
                     IYMLTGERTKAKNLLRKIERSEGEFTEEAQELLQKEFND"
     gene            5208..6434
                     /locus_tag="PKY63_10705"
     CDS             5208..6434
                     /locus_tag="PKY63_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF01127.1"
                     /translation="MIKFFLSGFLLSIGLMSTAQTISGIIEDADGKPVSGAHIYCNDD
                     DIGVVSDSKGQYIFNSKGCSLIIISHISYEQKTISATELQRNPNIVLNESAYLQKPID
                     IYSKPIVALLPDTPLFVHDYEISDERIFLSAFFQRKINQSVLLYTDLNGRIIDSETLS
                     ETAELYRDPEGAIYVTQKNYAYQLFTNNNQITFSESFESKYVTAAREHWTHSVGDSVV
                     LQYYYFKNQGVGYFIKQIPEDSARLFLEFIDESAYERMAWGPYFDGNEFDQRFEELIV
                     YKPVQIPIFFRINDIVAFNFISWKIQYFDLEGQLLSEIDMQYGEKNKIQKEIYFDSKS
                     GKYYGREIRNGLNSLIEIDISTGKMIKFFTFKGYPHIEKLCVSNGVLYFIYKDYSGDE
                     YKRLYKSPLTNMVAGR"
     gene            6666..7949
                     /locus_tag="PKY63_10710"
     CDS             6666..7949
                     /locus_tag="PKY63_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPF01128.1"
                     /translation="MALIILLLLLLNGVFAMFEIAMVSSRRARLQERASNGSNGAALA
                     LKLLKDPDKMFSAIQVGITLIGIINGALGGVSFTDDLTPHIASINGLEQYAPTISMLI
                     VIGMITYLSLVIGELVPKSLAMNNAEPIAIFLSPAMNLLRRLMYPIVWLLSISTKFIL
                     KLIGVKNRNEPPVTDEELRFMLKIGAESGVFNKEESVLINEVLRFGDKKATHVMTQRH
                     DVDFLNLNSSPAEILEMAGNTMYSKLLVIDRSIDNIAGVVSVHDILKQYIAKKTIRLA
                     DILTDPVYIPEQMLAVRMIELFQRTKNHFGVVVDEYGTMSGIVTLHDLIENIMGDLPL
                     LDDLDEPEIMQREDGSWLADGGMLWSELAEKLNVKVPTDEEELLSGINTLGGYAMVKL
                     NKIPSAGDVFHAAGFRFEIMDMDGKRVDKILISGE"
     gene            8188..8553
                     /locus_tag="PKY63_10715"
     CDS             8188..8553
                     /locus_tag="PKY63_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF01129.1"
                     /translation="MKLCWVTIHVTNMQQSKQFYCDVLGLKIKREFAPGPGFSICFLE
                     GNGADYELIEDASKLNEVSFSNSVSVGFLVDSLDEKMADLRSKGVENIIGPIAPSPND
                     RFIFIKDPDGMTVQIVEHK"
     gene            8612..9055
                     /locus_tag="PKY63_10720"
     CDS             8612..9055
                     /locus_tag="PKY63_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01130.1"
                     /translation="MRIVISLLLVLSLFFSSCDNKKANDKSSGNDIHLSKKEFYEHKM
                     KEKNFQLKMLGPCEDVPEDTPGETQDVFIDSKNITGTNAVVEFRFVDDCCMEFSGDYN
                     IQNDTLTFQYEQINDFKCDCYCLYKYRLTINKTKVPFHAIEIKQK"
     gene            9127..9426
                     /locus_tag="PKY63_10725"
     CDS             9127..9426
                     /locus_tag="PKY63_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPF01131.1"
                     /translation="MNLISGNIDKIQDLCRQHKVSRLFVFGSVLTSRFKKNSDIDLVV
                     DFAEMDRHDYADNYFDLKESLEKLFKRNVDLLEEKAIKNPYLKPGVSGYKSNKSS"
BASE COUNT         3004 a         1890 c         1972 g         2577 t
ORIGIN      
        1 tattaacaaa aatggcaata ataattttga ttttgaaatt ttagacagtt actaaccgat
       61 ttttgcaaaa cagaattgat tctacgcaat tcatgttgca aatgcaaatt tgacagatac
      121 aataaaaaac tccatattaa acaaagtaca tccgaaaaat ctgaactcgg gaatctgttt
      181 cgcaaggttt tgtaacttta cagcaaaata ttcagatgag gttttcattc ttcctgttaa
      241 ttgcatttct atgcacatat gcttacgcac agcaacaaac accaaagaac atcatcattc
      301 tcatcgccga cggtggtggt tataatcaga tgatgtgtac cgacttcttt atgtatggag
      361 actatccaaa gtcgccactg gctgcgtttc cggtgcagtt agccatgagt acgtataacg
      421 gctctttaca ttctcatggc gcaacatgct acgacagtaa aaaattctgg accgatttca
      481 gttacggaat ggccggattc acagaatctg ctgcttcggc aacagccatg ggaacagggt
      541 tcaagacaca caacagcatg attggaatga atctcgatgg gaatccaatg accaatgtga
      601 tagaaatagg aaaatcaaaa ggcaagaaaa caggagttgt aacttcggtt cctttctgtc
      661 atgccacacc agccggtttt tcgacacatt ccaaatcccg tcatggtttt tctgaaatca
      721 ctggccagat gctaaattca ggcattctgg atgtaattgg cggatcagga cacccctact
      781 tcgacaatga tggtaatgct gttcaaaagc catttacaaa atatattgat actgcatcgt
      841 ggaagaatct gaaagcagga aaaggcaaca tcaagtggtt ttacaccgaa agccgcagcg
      901 aaattcaaac acttgctgat cgaaaaactg cgcctgaaaa attattcatg ctggctccgg
      961 tatccggaaa tctggccgaa gccagagatg gccattcaat catacccttc gatattcccg
     1021 ttaaacaatc cattccaaca cttgccgaaa tgtcgctcgc agcgctgaat acattaagta
     1081 cagacccgga tggttttatt ctgatgattg aagggggtgc catcgattgg gccgaccatg
     1141 acaacaatat gccccgcatg attgaagaat acatgtattt ttacaacacc gtagattccg
     1201 ttcagaaatg gctgaaagaa aaaggattgc tggaagaaac gcttattatt gttaccaacg
     1261 accatgaatg cggctatctg tgggggccag gctgtgccgg atcaactttc agtttgccgc
     1321 aaaacaacgg aaaagggaat ttgccaactt tcaaatatta cagcgataat cactcaaaca
     1381 cactggtgcc cttctttgcc aaaggtccat cctctgaaat attttatgct ctggccgatg
     1441 aaaacgattc cgttcgtgga ccatatctca ccaattcaga aatcgccatt gcagtaaaaa
     1501 tgttgtgggg caatgctgtc gaaatctatg aaagctcttc atcaaaaaaa gacagtctga
     1561 ctctgtttgt aacactcccc tgcgctgatg ccagcattca atggaaagcc aataacacag
     1621 acatttccgg cgccaataca gtgcaatttt ttatcgaccc caacgtttat acaaaacctc
     1681 tgtcaatcag ctgcgaagtt acctgtggtc aaacaacatt cacaacacaa gaatatgagt
     1741 acaaacctta agcaattctg ttttttactt tttgcaatta ccacgctgag tatttctgcc
     1801 caaaatgatg acataggcag cggactgaaa aatcatttat tcaccggagg agctctgggt
     1861 cttacttttg gtaacgaaac caatatcgaa gcagccccgt tattcggcta tcatttgtcc
     1921 aatgtagtca gctttggaat tggcggcacc tatcagtatt atcattcgag atattttaac
     1981 gagagcatga atattttcgg aggccgtgct tttttacgca tccagccact gaagccggtt
     2041 tttctgcatg cagaatatca attcctgaca tacaaaacaa atatattcaa cgcgccaacc
     2101 tatcaggtgc agcaaattgt tgcagaagga ttgctggtgg gggccggcta ccgtgaatat
     2161 ctttccgacc gcctcagtac agtgattatg ctgctgtatg atttgaatta caccaagtac
     2221 tcaccgtatt ccaatccatt gttccgcttt ggtattgagt atgccattcc gctgaaagac
     2281 tgactttttt cgttacatgt tacgcgtgga atttattccg ctggaagcgg aaatcgttac
     2341 attttagcag tcagtagtca gctttcagca gcaggtgccg acctaagcgt cggcgctctg
     2401 agaagaagct gagcagaata tgctctttac tggcgcggat atttgatccg agacagtgaa
     2461 ctcatagcac gaattataaa tccgcgctat gagagggctc tgaaaggtcg aaagcaagtt
     2521 gctgaatagt gaacagagcg aaagccatgc ctttgcaaca ttttgcaagc gtttgtgtca
     2581 cccttcgaca tgctcataac gagtctttcc actttaaaaa cttgatgaac ttttaactcg
     2641 cgcagcgttg cgccgttagc tgcaccgttc caaccgataa ctgacgaaaa gcgacctttt
     2701 agcaaaaaaa tgctattttt acacttgaaa agtatggaaa aacttcagca ctgcatcttg
     2761 aatgacaaat taaccaagtc atgaaaacac aaattctatc attagccatt atgctcgtgc
     2821 tgagcattgc ctccataagc cctgtttttt cgcaggccgg cggcaccatt aaagggactg
     2881 tcattgaaaa tatcagtgga aatcctattc ctttcgtaag tgtattcgta aaatacggcg
     2941 acaatctgat tggtgctcaa agcgattttg acggaaactt tacaataaaa cctgttcctc
     3001 cgggaactta ttctgttaca tttaaatgtg ttggatatgg tgacaaaatt gtaaccgacg
     3061 ttgtaattac acaggataaa tacacttatt taaatgaagt tacaatgagc gctggaattg
     3121 atattcccgt cgttgtcatt ccagcaaata aaatgattga tcctgaatta accagtctga
     3181 aagttatcga ccaaactttt attgaatcgg ttccaaacta tggcaacata aacaaactgg
     3241 ttgcaatttc ttcaagcgac attcaggtaa gtgatgacga cagtgagatt tattttcgcg
     3301 gttcacgttc aggcgatgcc ctctacatca ttgatggcat gcggatgata aatccgggcg
     3361 cactttgtcc ttcgctggcc attggaagta tgtcggttta cacgggtggt gtaccagcca
     3421 aatacggaga tttcacaggc ggagtcattg tcattgaaac acaaagttat tttgaatggc
     3481 tggccgctca ggaatcagaa agattgcgaa atggaggttt ttaaaatttg atatatgcaa
     3541 tttctgttga acaggacaaa taagaattta gctgatgaag agctggtttc acgctttgcc
     3601 gaaaatggca atccatctat cattggcatc ctgttcgacc gatacgcaaa actggtcatg
     3661 ggggtatgcc ttaaatacct gaaagatgaa gatgaagcgg ccgataccac catgaccatt
     3721 tttaagaacc tgctggaaac gcttggcaat tacgatgtgc gggcattcaa accctggctc
     3781 tacacctacg ccaaaaacga atgtctgatg aaactccgca aagcaaaacc agtagtctgc
     3841 tccatcgaca aagtcgcatt tggtcttgcc ggagaagaag atgacgaaga gaggatcgac
     3901 cttccgatag agcagttgca gagcgccatc gccgccctga aaaacgacca gcaggtatgc
     3961 atacaaatgt tttttcttga aaatctttca tacaatgaaa tcagcgacaa aacgggatat
     4021 agctggaaag aaatcaagag ccatatacag aacggaaaaa gaaatttgcg aaacatatta
     4081 atcagcaccg gaaatgaata acaagactta catatcgccc ccggaacaag aggacgatcc
     4141 attcctgagg gatgcacagg aaggttggac acagttcccc ggcgcacggt cgcggtggtg
     4201 gaagaagaaa ttacagttca atctgtttct tttcggaaaa tcgtggagtg tattgccggc
     4261 tgccggcaaa cttgccattg gaacgctttc atcggttgcc ttaataaccg ttgcggccgt
     4321 aaccattcca ttcaattttt ccagcaacaa acaagaactg gcacaacagg aaaccacaag
     4381 ccagaaagat aaaataattc cctcagaaga aataattaca aacgaaaatg tgtctcagga
     4441 agaaacaaaa acagaaggat tcactaccat cacaaatgaa gaacaaaaca gcaaccatac
     4501 aataattcaa aacaatattc ctgttgctga agatgaaaca gagcaggcaa ctgtttatga
     4561 ctggcgcgac tatgatatgg aaaccgacaa gatttccgaa acagaaaaat acaccgaatc
     4621 aaatgcacgc atgcatccgg tgcaggccaa aaaagtgaac aatgaaatat caggcggact
     4681 gaaaaatgcg cttccttacg gctatatcag ccagtaccga tatcttgact atgacgcact
     4741 caataacgat ataccgcaag cccctacatc ggtaacaacc ggaagtcttg atgcaaaatt
     4801 cagtaatcat ttggaaaaag aaaaagttga agcatctgtc gttttggata ccattcctta
     4861 taaaactttc gtgcaggatg ctgttacaaa aataaaagac ggcaaatatg cagctgccga
     4921 atatcaattt aagaaactgc ttgtacaaag acccacagac gaaaacgcta ttttttatct
     4981 tgggtattgt ttctttcagc aaggtaatta tccactggca ctgagttatt ttgaaaaaac
     5041 aaaaaagaca aaatacatgg cttttatgag tgaagcagac tggtacacag cacacatcta
     5101 tatgctcact ggcgaacgta ccaaagcaaa aaacctgctg agaaaaatag aaagatcgga
     5161 gggagaattc actgaagaag cacaggaact gctgcaaaaa gaattcaatg attaaatttt
     5221 ttcttagcgg ttttctgctc agcattgggc tgatgtcaac tgctcaaacc atcagcggca
     5281 ttatcgaaga tgctgacggg aagccggttt caggagcaca tatttactgc aacgacgatg
     5341 acattggtgt agtatcggac tcaaaaggac aatacatttt taattcaaaa ggatgttctc
     5401 tgattataat cagtcacatc agctatgagc aaaaaacaat atcagcaact gaacttcaga
     5461 gaaatcccaa cattgttctc aatgaatctg catatctgca aaaacccata gacatatatt
     5521 caaaaccaat tgtggcgttg cttcccgaca cacctttatt tgttcatgat tatgaaataa
     5581 gtgacgaaag aatttttctg tctgcctttt ttcagcgaaa aataaaccaa tccgttttac
     5641 tttacacaga tctgaacgga cgtattattg actcagaaac actttctgaa actgcagaat
     5701 tataccgtga tccggaaggt gccatttatg tgacacaaaa aaactacgct tatcagttgt
     5761 tcaccaacaa caaccaaatt actttttcgg aatcattcga gtcaaagtat gtaactgctg
     5821 cacgtgagca ctggactcat tcggtcggag acagcgtggt gctgcaatat tattatttca
     5881 agaatcaggg agtcggttat ttcatcaaac aaatacctga agacagcgcg cgtttatttc
     5941 ttgagttcat tgatgaaagc gcctacgaaa gaatggcatg gggaccatat ttcgatggaa
     6001 atgaatttga tcagcgattc gaagaactca ttgtgtacaa accagtgcaa ataccaattt
     6061 tttttcgcat caatgatatt gtcgcattca actttatcag ctggaaaatc cagtacttcg
     6121 atcttgaggg ccagttgcta agcgaaattg atatgcagta tggtgaaaaa aacaaaattc
     6181 agaaagaaat ttattttgat tcgaaaagcg gaaaatatta tggacgtgag atcaggaacg
     6241 gcctcaatag tctgattgaa attgatatca gcaccggaaa aatgattaag ttttttactt
     6301 tcaaaggata tccacatata gaaaagttat gtgtttcaaa tggggttttg tatttcattt
     6361 acaaggatta cagcggcgac gaatataaac gattgtataa aagtccgctg acaaatatgg
     6421 ttgcaggcag gtaggcgtgc aatgcgcaat aagtttataa agttcataaa gttttaaagt
     6481 ggaaaggtga cgtataatta tgaggcatag ggcatgctgc tatatgcgta ttgcttctta
     6541 gttgaaagtt catttcccct gacgattcca ggtcagcaat gcctgttggt caaccactaa
     6601 tagctgacgc taaaatgtca gggatatcac ccggaattcg tatttttgta ttataaaatc
     6661 acgaaatggc attgattatt ttattattgt tactgctcaa cggggtgttt gccatgtttg
     6721 aaattgccat ggtttcatca agacgtgcac ggcttcagga gcgggcttcg aacggaagta
     6781 atggggctgc acttgcatta aaattgctga aagaccctga caaaatgttt tcagccattc
     6841 aggttggaat tacattgatt ggaattatca acggtgcact gggtggagtt tcctttaccg
     6901 acgacctcac tccacacata gcatctataa acggattgga acaatatgcc cctaccattt
     6961 ccatgcttat tgtcattgga atgataacat atttgtcgct tgttatcggc gaattagtgc
     7021 ctaaatcact ggccatgaac aatgcagaac cgatagccat ttttctgtcc ccagcgatga
     7081 atttgttacg cagattaatg tatccgattg tctggttgct ttcaatttca acaaaattca
     7141 ttttaaaact gataggggtt aaaaacagaa atgaacctcc ggtaacagat gaggaattac
     7201 ggtttatgct gaaaatcggc gctgaaagcg gcgtttttaa taaggaagaa tccgtcttga
     7261 ttaatgaagt gcttcgcttc ggggataaaa aagctacaca tgtaatgact caacgacatg
     7321 atgttgattt tctgaatttg aattcctcac ctgcagaaat tcttgaaatg gcaggaaata
     7381 caatgtattc caaactgctg gttattgacc ggagcatcga caatattgcc ggagttgtat
     7441 cagttcacga cattctcaaa cagtacattg cgaaaaaaac tatacggctt gcagatattt
     7501 taacagatcc tgtttatatt cccgagcaaa tgctggcagt gcgtatgatt gaattgtttc
     7561 agcgcaccaa aaatcacttt ggtgttgttg tggatgagta tggcaccatg tcgggcattg
     7621 taacattgca cgatttgatt gaaaacatca tgggcgatct gccgctgctt gacgatttgg
     7681 atgaacccga aatcatgcag cgcgaagatg gctcctggct tgctgatgga ggcatgctat
     7741 ggagtgagtt ggctgaaaag ctcaacgtaa aagtccctac ggacgaagaa gaacttctga
     7801 gtggaattaa tactcttggt ggctatgcga tggtgaaact caataaaatt ccatcagccg
     7861 gcgatgtgtt tcatgctgcc ggattccgct ttgaaattat ggatatggac gggaagagag
     7921 tggataaaat tcttatcagt ggtgagtagt gaatggtcag tagccagtaa tcagtggtca
     7981 gtggtcagtt agattgaggt tgaagttaag gttgagcagc tttcagctga ggttcaggat
     8041 gacagcgggc agggcgcagc gtgacagctg cggggctcag agttcaaaat caattggatg
     8101 gttttgcatg atcagaatac aataatcagt atttttgtgt tgtatcaata aaaaaacatt
     8161 attaattctt caaaaaacac aaaacctatg aaactctgct gggtaaccat tcatgtgacg
     8221 aacatgcaac aatcaaaaca attttattgc gatgttcttg gattgaaaat caagcgggaa
     8281 ttcgctccgg ggcctggttt cagcatatgc tttctggaag gtaacggcgc cgattatgag
     8341 ttgattgaag atgcctcgaa gctcaatgaa gtttcattca gcaacagcgt ttcggttggc
     8401 tttctcgtag attcgctcga cgaaaaaatg gccgatctca gaagcaaagg cgttgaaaac
     8461 atcatcgggc ctattgcgcc atcgcccaat gaccgcttca tttttatcaa agatccggat
     8521 gggatgacgg tgcagattgt ggagcataag tgaatcatgt aattgcatac agaactacat
     8581 cagataatac tgattcaaaa tcaggaataa aatgagaatt gtcatatcgt tactattggt
     8641 attgtcctta ttcttttcat catgcgacaa taaaaaagca aatgacaaga gctccggaaa
     8701 tgatattcat ttatcaaaga aagaattcta cgagcataaa atgaaagaaa aaaactttca
     8761 gttgaaaatg ctgggcccat gcgaagatgt gcccgaagac acacctggag aaactcagga
     8821 tgtttttatt gactccaaaa acattaccgg aaccaacgcg gtagttgaat tccgatttgt
     8881 tgatgattgc tgcatggaat tttcaggaga ttataatatc caaaacgaca cgcttacttt
     8941 tcaatatgag caaataaatg acttcaaatg cgattgttat tgcttgtaca aatacagact
     9001 gaccataaac aaaacaaaag taccctttca tgccattgaa ataaagcaga aataacgaaa
     9061 tgtttaatat tattttaacc caattacaaa tttgacattt gcgcttattt ttattatctt
     9121 tgtaacatga atctgatctc cggaaacatt gacaaaattc aggacttgtg ccgccagcat
     9181 aaagttagtc ggctatttgt tttcggatcg gttctgacca gccggttcaa aaaaaacagc
     9241 gatattgacc tggttgttga ttttgctgaa atggatcggc atgattatgc cgacaactat
     9301 tttgatttga aagaatcgct ggaaaaactt tttaaacgca atgtagattt acttgaagaa
     9361 aaagccatta aaaacccgta tttaaaacca ggcgtgtccg gttataagtc aaacaaatcc
     9421 agttgattag gatcgcgtgt ttg
//
LOCUS       DAOPKE010000100         9409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7667_length_9409_cov_10.210498, whole genome shotgun sequence.
ACCESSION   DAOPKE010000100 DAOPKE010000000
VERSION     DAOPKE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7667_length_9409_cov_10.210498"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1370
                     /locus_tag="PKY63_10730"
     CDS             33..1370
                     /locus_tag="PKY63_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPF01132.1"
                     /translation="MKRNVIIIGAAGRDFHNFNTFFRGNAAYNVVAFTAAQIPDIDGK
                     KYPAELAGKELYPQGIPIHSQKDLIKLIKEHNVDECVFAYSDMPYDYVMDLASEVNAA
                     GADFNLMGLKTTMLKSTKPVIATGAVRTGGGKSEASRRIVDELKALGLKVIAIRHPMP
                     YGDLVKQAVQRFATVEDLKKHECTIEEMEEYEPHVVRDTVIYSGIDYEAILRQAESEN
                     PDVILWDGGNNDFPFIKPDLMITMADAQRAGDEMYYYPGSTTLRLADVVVFNKIESAD
                     LEDLLMVRENVAKINPKAITMDACSPTFVDNPEVIRGKRVLVVEEGPTVTHGGVQNNY
                     GALTAMKYGAAELVDAKPYAVGKLKETYDNYPDLGPILPSMGYSKQQVEDLEKTIEKT
                     PCDLVLIATPIDINRIIKIKKPTVKIGYEMQEIGTPDFKQVIGDFAKKHNLKK"
     assembly_gap    1371..1470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1471..2310
                     /gene="pyrB"
                     /locus_tag="PKY63_10735"
     CDS             <1471..2310
                     /gene="pyrB"
                     /locus_tag="PKY63_10735"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPF01133.1"
                     /translation="KKFEENPNRKILEGKVVGVLFFEPSTRTRLSFEAAINRMGGRVI
                     GFSEASSTSVQKGESLKDTIQTVNQYADLIVMRHPVEGSARYASEVATCPIINAGDGA
                     NQHPTQCLLDLYTILQTQGTLDNLNMTFVGDLKYGRTVHSLSIAMTMFNTTFHLVSPE
                     ELKLPSAVKIHIKDKKLPYHQYTDLNEAIPLSDIIYMTRIQAERFSDPMEYERVKNSY
                     VLNRELLSNAKPGMKVLHPLPRVNEIALDVDDSPQAYYFKQAQNGMFVREALIAHILG
                     LNV"
     gene            2323..2787
                     /gene="pyrI"
                     /locus_tag="PKY63_10740"
     CDS             2323..2787
                     /gene="pyrI"
                     /locus_tag="PKY63_10740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPF01134.1"
                     /translation="MTKNDNHSLQVKALENGTVIDHIPQGVVQRVLNILGLEDFTEQI
                     YMGANLESQKMGRKGIIKVANHFFGQEEIDKLGLIAPTATVIEIRDYEVKSKTKVHIP
                     DEVHKMVKCFNPKCITNKEAVPTRFYVIDKEDLKLRCHYCEKITGRKNIEYK"
     gene            complement(2842..4929)
                     /locus_tag="PKY63_10745"
     CDS             complement(2842..4929)
                     /locus_tag="PKY63_10745"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPF01135.1"
                     /translation="MRKFLCSIITLFSAYAFLFASSADADLNSLILSNPRKAVELLNS
                     RIEKGLRKDDYHNVAPDLIHVASVYRMLGNFDDAMHSLLRAREIIVMHASSVSNTDSL
                     MGAYYETMGLIYIGRYMQTRGVSFLNEAITLYAKSGYYHGAATCMNWRNYAFLMINQP
                     DEAGKYLKQLFQWQQINRDPFIVACYNESEGAISELNGDYESAIKLYNIAMNNYRLCG
                     NVIGQSDMYAYICRALYTQGDYISTVKWAEKGAQFAKENGYSGGYYEILSYKIFALSK
                     SDMAMAFDLAKSQLDDISGLGLTIHKGIYLTNMISYAEKMNQYETAFKYSRQFLDVFK
                     EAYGLSSEQRIADMTMQMQTENLNLRIENLSTEKELNERIRLGQQNMIFYFVIALLVL
                     LLISVGNLKKLQFRMFLFRDYVSDLIPLPVFFLAFVGYGFLLLLLFKSPVLHSQAGFV
                     QYLHLLALSAIPSLIITLGLASLPAIWSSAPEKNRSFSRVTFSGLLLIYLLVIAYNLI
                     FGLIEFNLYEILNVIFTYTGLIVVPLFFIVIYIEKVLLRKRTQEAGFMNTKIRKSEMQ
                     LKPGVARIRIVSDKSKDVFESDSTDLLLVEGQSNYTKFYFIENELLKTSLLLMTLKQA
                     ESQLQSYKEFIRCHKSNIVNLTRVKKVRGNSHGYKLSVPPMEESVTVSKSYISEFNEA
                     FNRFSAGNAPLDD"
     gene            complement(5039..8263)
                     /locus_tag="PKY63_10750"
     CDS             complement(5039..8263)
                     /locus_tag="PKY63_10750"
                     /inference="COORDINATES: protein motif:HMM:NF026223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-repeat neuraminidase N-terminal
                     domain-containing protein"
                     /protein_id="HPF01136.1"
                     /translation="MKKILFAVVFLYACFSFSVAQVNTYTFAQGTGSPTYLSSGYTEI
                     GIGTGLDEDYHNNVPIGFYFNFNGIEYSSLTIHDNGYIVMGSYTGTTSSPISSVNNSI
                     SALGADLQGLSNATLRHQTLGSAPNRRFVAEWRHFETYGTYGGGDFSFQIILYEGTNV
                     IQFVYQTHTAFADDFQVGLRGNNSADYSNRKKDANANWTSSSAGTATSSVMLTGSTSA
                     LNPGGIYTWTPKNMIYVSSTTTQTVTTDVGLGASNQEIIGVQIVTQNTNNPLTVSSFT
                     INTNGSTAPAADISRMKLWSTGTSSTFATTNQIGNSISNPSGSIVINSGSNLPFTLVP
                     GTNYFWITYDIEASATINNYVDAECDSISVGGTPRTPTVQAPSGSRQIKDLCTSGIGT
                     GLVNIDSLPYNSGSQTTCGMVNNLKSSNTATCGDSYYLSDEDVVYVFTPAVSGCITIN
                     LTSSKTYSGLMLYHGCPINGVGGTCVSYQQSSTADKTMTAGVIAGETYYLVIDSWTLN
                     TPHCLNYTVSISTPSGGPANSNPCNAVELPVGNYCSFNSYTNECSGGSLVPNPGCAGY
                     SGGDVWFKVFVPASGNIVIDTDDDVVTDGGMAIYSGADCNSLTLLACNDDGSENGYMP
                     MISLSNQTPGAPLWIRFWEADNNNNGTFGICAYQMPDVPVCVSNPAAGDHCTDATPIC
                     NLNGYCGNTSATYDSDEPGNLYDLWVSATIENNSWLKFVAENDTVVLNVWVSNCVYGD
                     GIQMRIVGTSDCNAFAAYSNFWNPEVETDGIIMATGLTIGETYYLVIDGYAGDVCDYV
                     IGAGVWSGVLAPNAGADTVITAGACATLSATGGTGFQWSSNPVDPTLSVAEQTQQTIN
                     VCPTVQTTYTVTVTGGNPLCPSNGTDEAVVYINSGLPVDYLFYNALCSGDEVTIDWST
                     ASETNCDYFLIEKSVDGELFYGIKTMAGHGNSNDIVHYSVKDADIHSSENYYRIKQVD
                     FDGQYNYSPVMTAICKDNSNQSDLFVNYSNESMIVHFDAVDGEQYVICVYDVQGRKIH
                     EEMYVAGDDETADIELLLVKPLSGVYLVSVESLSFSGYQKCIIQ"
     gene            complement(8266..8865)
                     /locus_tag="PKY63_10755"
     CDS             complement(8266..8865)
                     /locus_tag="PKY63_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01137.1"
                     /translation="MKISLVLFVFLSLVVLNDCGAQTQGVWIRFDGADVYLPSDYIDP
                     SYSEAIRKSANPDDQEYASYLHDHLFPLPDEADYERKIEYWLLHNRKYYPQFLSTGNP
                     TLDSLRMLSAQEFWKLKNEKLLLQLSGYNNPTAYSDEEYRALCNSFPNRDDFEQSEAG
                     SLRYQSAVQEWIRIHSMQAYLAIQSVVENHNDYSKTGRE"
BASE COUNT         2939 a         2070 c         1801 g         2499 t
ORIGIN      
        1 tttgcagcaa attcaaaaca aaacgttgaa cgatgaaaag aaatgttatt atcattggag
       61 ctgctggacg agatttccac aacttcaaca cctttttcag aggaaatgct gcatacaatg
      121 tagttgcttt tacagctgca caaattcctg acattgatgg aaaaaaatat ccagcagagt
      181 tggcaggaaa agaactttac ccacaaggta ttcctattca ttcacaaaaa gatttaatca
      241 agttaatcaa agaacataat gttgatgaat gcgtttttgc atatagcgat atgccttatg
      301 attacgtaat ggacttagct tctgaagtta atgctgctgg agcagatttc aacctaatgg
      361 gattgaaaac cactatgcta aaatcaacaa aaccagtaat tgctactgga gctgttagaa
      421 ccggtggtgg caagagcgaa gcatcaagaa gaattgttga tgaactaaaa gccctaggtt
      481 taaaagttat tgctattaga cacccaatgc cttatggcga cttagtaaaa caagctgtac
      541 aacgctttgc tactgttgaa gacttaaaga aacacgaatg taccattgaa gaaatggaag
      601 aatacgaacc acacgtagta cgcgacacag ttatttactc tggtattgat tatgaagcta
      661 tccttcgtca agctgaaagc gaaaacccag acgttatttt atgggacggc ggaaacaatg
      721 acttcccatt cattaagcct gacttgatga ttacaatggc tgatgcacaa cgtgctggag
      781 acgaaatgta ttactatcct ggctctacca cactacgcct tgctgatgta gttgttttca
      841 acaaaattga aagcgctgac ttagaagacc tacttatggt aagagaaaat gtggctaaaa
      901 tcaatcctaa agccattaca atggatgctt gctctcctac ttttgtagat aatccagaag
      961 ttatccgtgg aaaacgcgta ttagttgttg aagaaggtcc taccgttact cacggcggcg
     1021 ttcaaaataa ctatggtgct ttaactgcta tgaaatatgg tgctgctgaa ttagttgacg
     1081 ctaaacctta tgctgttgga aaattaaaag aaacctatga caattatcct gacttaggtc
     1141 caatccttcc ttcaatgggt tacagcaaac aacaagtaga agacttggaa aaaactattg
     1201 aaaaaacacc atgcgattta gtacttatcg caactcctat tgacataaac cgtatcataa
     1261 aaattaaaaa acctacagtt aaaattggat acgaaatgca agaaatagga acaccagact
     1321 tcaaacaagt tattggcgac tttgctaaaa aacacaattt gaaaaaataa nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaaattcg aagaaaatcc aaatcgcaaa
     1501 atccttgaag gaaaagttgt tggcgttttg tttttcgaac cgtccacacg tacacgtctg
     1561 agttttgaag cagcaatcaa ccgcatgggc ggtcgtgtta tcggcttttc cgaagcatcc
     1621 agcaccagtg ttcaaaaagg cgaatcgctg aaagacacca tccagaccgt gaatcaatat
     1681 gccgatctga ttgtgatgcg acaccctgtc gaaggcagcg cacgctacgc cagcgaagtg
     1741 gccacctgcc ccatcatcaa tgccggtgac ggtgcaaatc agcaccccac ccaatgcctg
     1801 ctagatcttt ataccattct gcaaactcaa ggcacccttg acaatctgaa catgaccttt
     1861 gtaggcgacc tgaaatacgg acgtaccgtt catagtctga gcattgccat gaccatgttc
     1921 aacaccacat ttcacctggt ttcgcccgaa gagctcaaac tgccgagtgc tgtaaaaatt
     1981 cacatcaaag ataaaaaact tccgtaccat caatacacag atctgaacga agccattccc
     2041 ctgagcgaca ttatttacat gacacgcatt caagccgaac gtttttcaga tcccatggaa
     2101 tacgaacggg taaaaaacag ttacgtcctc aaccgcgaac tgctcagcaa tgccaaaccc
     2161 ggcatgaaag tgcttcatcc gctgcccagg gtaaacgaaa ttgcacttga tgtggatgac
     2221 agtcctcagg cctattattt taaacaggca caaaacggaa tgtttgtgcg cgaagcactg
     2281 attgcacata ttttaggact aaacgtataa ggagaaaaca ccatgactaa aaacgataat
     2341 cactcattgc aggtaaaagc ccttgaaaac ggaaccgtca ttgaccatat tccacagggc
     2401 gttgtacagc gcgttctgaa cattctcgga ctcgaagatt tcaccgaaca gatttacatg
     2461 ggagccaatc tcgaaagtca gaaaatgggc cgcaaaggca tcattaaagt ggccaatcat
     2521 tttttcggtc aggaagaaat tgacaagctg ggtctgattg caccaaccgc cacggtaatt
     2581 gaaatccgcg actacgaagt aaaaagcaaa accaaagtgc atattcccga cgaagtgcat
     2641 aagatggtga aatgtttcaa tcctaaatgc atcaccaaca aagaagcggt tcctacgcgc
     2701 ttctacgtaa ttgacaagga agatctgaaa ctccgctgcc actattgcga aaaaatcacc
     2761 ggacgaaaga atattgagta taaataagaa ctctccactc ttccgaaagg ctttgattct
     2821 tttgattcga agcctttttt tttaatcatc caaaggcgca ttgccggccg aaaagcgatt
     2881 gaacgcctca ttaaattcac tgatgtaact tttcgaaacc gttactgatt cttccatcgg
     2941 aggtactgaa agcttgtatc catgagagtt tccacgcact ttcttcacgc gggtcagatt
     3001 cacaatattg gatttatgac atcggataaa ctccttataa ctctgcagct ggctctcagc
     3061 ctgctttaac gtcataagca acaacgaggt cttcaataat tcattttcaa taaaataaaa
     3121 cttcgtgtag ttgctctgtc cttccacgag caacaaatcg gttgaatcac tttcaaacac
     3181 atctttcgat ttatctgaaa cgatacggat tcgtgcaaca cctggtttca gctgcatttc
     3241 gcttttacgg attttcgtat tcataaaacc ggcttcctgc gtacgtttgc gcaacaatac
     3301 cttttcgatg taaatgacaa taaaaaataa cggaacaaca atgagtcctg tataagtaaa
     3361 aatgacattc agtatttcat acagattaaa ctcaatcaaa ccaaaaatga gattgtatgc
     3421 gatcaccagc aaatagatca acaataatcc gctaaaagtc acccttgaaa agcttcggtt
     3481 tttctcaggc gccgacgacc agatggccgg cagcgaagcc aggcccagcg ttattatcag
     3541 cgaaggaatt gccgacagcg ccaacagatg cagatactga acgaatcctg cctgactatg
     3601 taaaaccgga cttttgaaca acagcagcaa caaaaaacca taacccacaa aagccagaaa
     3661 gaaaaccggc agtggaatca gatcgctcac gtaatcacgg aaaagaaaca tccggaattg
     3721 cagctttttt aaattcccga cagaaatcag aagcagaaca agcaatgcaa taacaaaata
     3781 aaaaatcata ttctgctgac ccagacgaat acgctcgttg agttcctttt ccgtgctcag
     3841 attttcaatg cgtaagttca ggttttctgt ctgcatctgc atagtcatgt ctgcaattcg
     3901 ctgttcgctc gataatccat atgcttcttt aaaaacatcg agaaactgac gtgagtattt
     3961 aaatgcggtt tcatactgat tcattttttc agcgtagctg atcatgttgg tcagataaat
     4021 ccctttgtga attgtaagtc ctaatccgga aatatcatcc aactgacttt ttgccaaatc
     4081 gaatgccatc gccatgtcac ttttcgaaag cgcaaagatt ttgtatgata aaatctcata
     4141 ataacctcca gaataaccat tctctttcgc aaactgtgcg cccttttcag cccattttac
     4201 ggttgatatg taatcaccct gagtgtataa tgcacgacag atgtaggcat acatgtcgga
     4261 ctgaccgata acatttccac acaggcgata attattcatc gcaatattat aaagcttaat
     4321 ggctgattca taatcgccat tcagttcaga aatggctcct tcagattcat tataacatgc
     4381 cacaataaat ggatcgcgat ttatctgctg ccactgaaac aattgcttta aatatttacc
     4441 agcctcatcc ggctgattaa tcatcagaaa agcataattg cgccaattca tgcaggtagc
     4501 agctccatga tagtacccgg atttggcgta caatgtaatg gcttcgttca aaaaactgac
     4561 gccgcgtgtc tgcatataac gaccaatgta gatcaatccc atcgtttcgt aataggcccc
     4621 catgagtgag tcggtatttg aaacagagga agcgtgcatc acaattattt cgcgggcacg
     4681 cagtaagcta tgcatggcat cgtcgaaatt gccaagcatg cgataaacag aagcaacatg
     4741 aatcagatca ggagcaacat tgtgataatc atccttccgc aatccttttt caatgcgcga
     4801 attcagcagc tcaaccgctt ttctgggatt cgataatatc agtgaattta aatctgcatc
     4861 agctgatgat gcaaacaaaa aagcatatgc ggaaaataaa gtaatgattg aacataaaaa
     4921 ctttctcata gctctctggt tctaattcag cataaaatta cagttttttt ataaaacaaa
     4981 accccgcaat cagatcgcag ggctttgagg tttgaaacgg ggggaataaa taacaacatt
     5041 attgtattat gcatttctgg tatccggaaa aagacaaact ctccacactg acaagataca
     5101 ctcctgataa tggtttaact aataataatt ctatatctgc cgtctcatca tcacctgcca
     5161 catacatttc ttcatgtatt tttctgccct gaacatcata aacacaaata acatactgct
     5221 ctccatctac cgcatcgaaa tgaacaatca ttgattcatt gctgtaatta acaaataagt
     5281 cagactgatt actattgtct ttgcaaattg cggtcataac aggtgaataa ttatactgcc
     5341 catcaaaatc aacttgttta atacggtagt aattttcaga agaatgaata tctgcatctt
     5401 tcactgaata atgaacaatg tcattggaat ttccatggcc cgccattgtt ttgattccat
     5461 aaaacaattc accgtcaact gatttttcaa tcaaaaaata atcacaattt gtttcagaag
     5521 ctgtactcca atcaattgtc acttcgtctc ctgaacaaag agcattataa aataaataat
     5581 caacaggcag tccgctgttg atataaacta cggcttcgtc tgttccattc gaagggcaca
     5641 gaggatttcc accggtgacc gtaacggtat aggttgtttg cactgtgggg cacacattaa
     5701 tggtttgctg cgtctgttca gctactgaaa gtgttgggtc aacgggattc gaagaccatt
     5761 ggaaaccggt tccacctgta gccgacaatg ttgcacaggc cccggctgtt atcacagtat
     5821 cagcaccggc attgggagcc agcacacctg accagactcc ggcgccaata acataatcgc
     5881 atacatcgcc ggcataacca tcaatcacca gatagtaggt ctcgccaata gtcagaccgg
     5941 ttgccatgat aattccatcc gtttccacct caggattcca gaaatttgaa tacgctgcaa
     6001 acgcgttgca gtcagatgtt ccaacgatac gcatctggat cccgtcgcca taaacgcaat
     6061 tcgaaaccca tacatttaaa acaacggtat cattttcagc aacaaatttc aaccatgaat
     6121 tgttctcaat ggtagcactc acccacaaat cgtatagatt acccggctcg tcactatcat
     6181 aggttgcact tgtatttccg caatatccgt ttaaattaca aatgggtgtg gcatctgtgc
     6241 aatggtctcc ggctgcggga ttgctaacac ataccggcac atcgggcatt tgatatgcgc
     6301 aaataccaaa tgttccgtta ttgttgttat cagcttccca gaaacgaatc cacaagggag
     6361 ctccgggagt ttggttgctc aatgaaatca tcggcatata accgttttca ctgccatcat
     6421 cgttgcaggc aagcaatgta agtgaattgc aatcggcacc actatatatt gccattccgc
     6481 catcggtcac aacatcatca tccgtatcaa tgacaatatt tccggaagca ggcacaaata
     6541 ccttaaacca tacatcgcca ccggaatatc ctgcacagcc cggatttggc accaatgaac
     6601 caccggaaca ttcatttgta taactattga atgagcagta atttccgaca ggcaattcaa
     6661 ctgcattgca tgggttgctg ttggccggac ctcctgaagg agttgagata gatactgtat
     6721 aattcaaaca atgtggggta ttcaatgtcc acgaatcaat caccaggtaa taggtttcac
     6781 cggcaataac accagcagtc atagttttat cggcagtact gctctgctga tacgaaacac
     6841 aggtgccacc aacaccattg attgggcagc catgatacaa catcagtccg ctgtatgttt
     6901 tacttgaagt gagatttatt gtgatgcagc ccgaaacagc aggagtaaaa acataaacaa
     6961 catcctcgtc gctcagatag tacgaatcgc cacaggtagc tgtattcgaa gatttgagat
     7021 tattcaccat gccgcaggtg gtctgtgatc cgctgttata tggcaaagaa tcaatattca
     7081 ccagcccggt accaatgcct gatgtacaca aatccttgat ctgcctgctc cccgaaggcg
     7141 cctgaaccgt aggcgtacgc ggcgttccgc caaccgaaat gctgtcgcat tcagcatcta
     7201 cataattatt tattgttgcc gatgcttcaa tatcataagt aatccagaaa taatttgttc
     7261 cgggaaccag cgtgaaagga agattagacc cgctgttgat tacaattgag ccagagggat
     7321 tgctgatgct gtttccaatt tgattggtgg tagcaaatgt tgaagaagtg ccggtgctcc
     7381 atagcttcat ccggctgata tcagctgccg gagccgtgct tccattggta ttgattgtaa
     7441 aagacgatac agtgagtggg ttgtttgtgt tctgagtcac aatctgaaca ccaataatct
     7501 cctgattaga tgcgcccaaa cctacatctg tagtaacagt ctgcgtggtt gtagatgata
     7561 catagatcat gttttttggg gtccatgtgt aaataccacc cggatttagt gctgaagtac
     7621 ttcctgtcag catcacgctg cttgtagcag taccagcaga agaagaagtc cagttggcat
     7681 tggcatcttt ttttctgttc gaataatctg ccgaattgtt tcctcgcaaa ccaacctgaa
     7741 aatcatcggc aaaagccgta tgggtttgat aaacaaactg aattacattg gtaccttcat
     7801 aaagaataat ctggaaagaa aaatcaccgc caccatatgt tccgtaggtt tcgaaatgac
     7861 gccactcggc cacaaaacgg cgattgggag cagagcccaa ggtctgatgg cgtaatgtcg
     7921 cattcgataa gccctgcaaa tcagctccca aagccgatat tgaattattg acacttgata
     7981 ttggtgaaga agttgttcct gtatagctcc ccataacaat gtatccatta tcatgtatgg
     8041 tcagcgaaga gtattctata ccattaaaat tgaagtagaa accaatgggg acattgttgt
     8101 gatagtcttc atcaaggcct gttccaatgc cgatttcggt gtaacccgat gacaagtagg
     8161 tcggtgagcc ggtaccctgc gcgaaggtat aagtattaac ctgagcaaca gaaaaagaaa
     8221 aacatgcata taaaaaaaca acagcaaaca aaatcttttt catagctatt ccctccccgt
     8281 tttagaataa tcattatggt tttctacaac tgactgaatg gccagatagg cctgcatgga
     8341 atgaatgcgg atccattcct gcaccgctga ctgataacgc agacttccag cttcgctttg
     8401 ctcaaaatca tcacggttcg gaaaagaatt gcacagcgcc cgatattctt catcggaata
     8461 tgctgtcgga ttattatatc ctgacagctg aagaaggagt ttttcgtttt tcagtttcca
     8521 gaattcctgt gctgatagca ttctcaagga atccaaggtt ggattgcccg ttgacaaaaa
     8581 ctgcggataa tactttctgt tatgcaacag ccaatactca atctttcttt cataatcagc
     8641 ttcatccggt agcggaaata aatggtcatg caggtacgat gcatattcct ggtcatccgg
     8701 atttgctgat tttctaatcg cttcggaata cgaagggtcg atataatccg aaggaagata
     8761 cacatcggcg ccatcaaatc gtatccagac accctgggtt tgcgccccac aatcattcag
     8821 cacaaccaat gataaaaaaa caaaaagaac tagtgaaatt ttcatacggt cgattgtttt
     8881 tggttactca tgccatcgtt ttgcctgcat gatccattat tttccatgca cacagacttt
     8941 gaacaaaaat acagcatcga ccgaccttta taaaatttag tacactcaat aacaacactc
     9001 tacgcgaaat aataaaacta ttgacgaatg aacagatttt atgtacaagc aatcagacgg
     9061 aaggttttta ttttatgcac ggcatcatat tcaattctgt ttttcaaagg caatatcagg
     9121 attaatatat tttatgaaga aaaaacactc catttattgc aaaaaaaatc ccgtgacttt
     9181 tgtctccggg attttgtact tatctgcagc tttacttggg ggagtatatt gggggggcag
     9241 ataagtcagg aattgcaaac aggtttcgct atctgaaatc gcaaatcctg agtaaatgta
     9301 gttttgataa tccgacagac aaaatccgtt ttaccaattt gcagaattta ttacgaatgc
     9361 ccagttttgc atgatgaaag gggctgactc tggataaaat accaggaac
//
LOCUS       DAOPKE010000101         9375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7719_length_9375_cov_8.565987, whole genome shotgun sequence.
ACCESSION   DAOPKE010000101 DAOPKE010000000
VERSION     DAOPKE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_9375_cov_8.565987"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2717)
                     /locus_tag="PKY63_10760"
     CDS             complement(<1..2717)
                     /locus_tag="PKY63_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPF01138.1"
                     /translation="MKKLLLMWIALLAFGVSFGQSITNYAFSYSFTGSLEDMSSGTTN
                     LMTGNNDDEASALTDIGFTFYFMGTPYTQFSVNSNGQLRLGSPIISSYSITSFSAGVP
                     ILAPMAGDNEVNNGIVYKVIGTAPNRILVVQWNEFAASYYTDASDGDMQVLLYEGSGK
                     IDFIYGEIYNSSSSAVTRSIFISSSNTASTAGYVTVGATPTFTPAASPTTNSFAASVL
                     IANLGSTTQGNRTVYTWTPPTTVTAPTNLTFTNVGASKMTLNWTDSPDETFYIISNSL
                     DGITYTPVTNLPANAVSYQANGLTPSTLYYWKVNATVESGLLSTDLTGSQSTLAQVPL
                     HGIYSINNLFPTTAVLLHDGTDNFNNFTDAINYLNDNGISASVTFAVAAGQTFAEDCP
                     AITASGTSVNKITFIKAGAGANPIISPTGTTATGEAGIAIVGGDYITFNAIDIAIASG
                     SNVEYGYYIANASATDGAQYNAIANCKVTLNRANTAARGISQYTPISATNASGANSYN
                     IYHKNIIENCYHGIQLTGTSSTYPDINCVIDSNIVGAATANDIGGGSSAANGIRANYQ
                     QNITIFGNIVRNVTASSSVYGIYAQYLLGTDNNIGNNIVYSINMTGTGTTTYVYGLRT
                     DINSGAMANIFNNMVSDLNHGITTASSNVVVRGIGVGLTGTGTSNIVHNSVLISEDAA
                     ASSAALYLGSSGTHNVLNNVFANTSATDATSKRYCIYRTTGATLYSDYNDLFVDAAGT
                     NNYAGYFTADRNTLADWQTASSQDAHSISVDPTFNGLTDLHTTNPALNGTAMLIPEIT
                     EDIDGEARDTYFPDMGADENLTPVVFTCTTPTPGNTIASSTALCYGDGVLLTLENATA
                     GTGVTYQWQSSTDGITYTNITGATAESYGTIQTAATYFQCVVTCHEGPVS"
     gene            complement(3034..3882)
                     /locus_tag="PKY63_10765"
     CDS             complement(3034..3882)
                     /locus_tag="PKY63_10765"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPF01139.1"
                     /translation="MKKLFQICIIAAIAIFTSCDVIEPPYTQTNNNPVVTDTVVRKVL
                     LEDYTGHQCPNCPGAAIIAHELMELYPGKISLITIHAGLFANLASPNYMTDFRCTTGN
                     DLNTYFGVDLIGNPNGTINRKEYSGSRIIGPNDWASKIAELLMLAPEADINTTHTFNT
                     TTSTIDLTAKVDVLADFSNPIMISAYLTEDSIVDYQKNNDPAVGTTPEIADYVHMHVL
                     RGSMNGTWGDTLCSGGATVGTQLSKTLSYTVSNASWNKDHMNIVVFIYDAATLEIIQT
                     EEVKLK"
     gene            complement(3896..5536)
                     /locus_tag="PKY63_10770"
     CDS             complement(3896..5536)
                     /locus_tag="PKY63_10770"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPF01140.1"
                     /translation="MKKFFAAAFILSSIAGAAQNVLQNAQIHGDFQTDFQYYLPDSSI
                     GADTVPEKLLSNGYLNLLFTSGNFSAGVRYENYNNVMQGFDPRYKGSGIPYRFASYSH
                     KDFEITAGNYYEQFGSGLLLRSYEERSLGIDNSLDGIRIRYKPYKGIYLKGAIGQQRF
                     FFDKGEGIVRGLDGEINLNEFNNKWAASKLKVTVGGSFVSKYQADRDPIYNLPQNVGG
                     WAVRTTLIYGNWMLNGEYAHKVNDPSTVNKMIYKPGEALFTSLSYSKKGFGITLSAKR
                     IDNMNFRSDRSATGNVLTMNYLPALTKPHSYTLLAFYPYASQPNGEMAFQGQLNYKIK
                     KGTKLGGHYGTDVAVNYSRANAIDMQKINDSTALMQSGTLGYTSDFFAIGDELYFEDI
                     NIEISHKFSKKVKGIFTWAYLTYNSDIIEGHTDGTFYSHIGVADVTYKITPTKSLRME
                     VQHLMTKQDEKNWANAMIEFTLAPKWFFMAGDSYNYGNPADDKKLHYYTTGFGYTKNS
                     SRIAITYGKQREGIVCVGGVCRNVPASYGFLVTITSNF"
     gene            complement(5554..6060)
                     /locus_tag="PKY63_10775"
     CDS             complement(5554..6060)
                     /locus_tag="PKY63_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPF01141.1"
                     /translation="MKSILVIIAFFAVIAMTAQNNTLPSVDLKKPDGTIVSSDSILNQ
                     GKPIIISFWATWCKPCVKELTTFAEVYEEWQEETGVKLYAISIDDSRTFPNVASFVNG
                     RDWPFDVFCDPNFDFKRAMNVNLVPHTFIIDKDRNIVWQHTSFTEGGELELIELIRKL
                     NNGEKISE"
     gene            complement(6303..6629)
                     /locus_tag="PKY63_10780"
     CDS             complement(6303..6629)
                     /locus_tag="PKY63_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF01142.1"
                     /translation="MENAFEHSKATTIAAAIDYTPGGIVSKIISKNTGGSLTLFSFDA
                     GQALSEHAAPFDAVVQVIEGTGEYIIGGVPHTVKAGELIIMPANIPHAVKAPEAFKML
                     LFMIKG"
     gene            complement(6706..7023)
                     /locus_tag="PKY63_10785"
     CDS             complement(6706..7023)
                     /locus_tag="PKY63_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF01143.1"
                     /translation="MKSTHYSTAGAKENPHGVDVKMLYNDPTAQALMISLQPGQALKP
                     HTTPVDVFFFVFEGTATVHIGEETESFTAGTLIESPKDIVHFLSNQSDNIVRVLVVKA
                     PKP"
     gene            complement(7079..8728)
                     /gene="hcp"
                     /locus_tag="PKY63_10790"
                     /gene_synonym="priS"
     CDS             complement(7079..8728)
                     /gene="hcp"
                     /locus_tag="PKY63_10790"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPF01144.1"
                     /translation="MSMFCFQCQETAKGTGCTIAGVCGKKEPVSAMQDLLIYNVKGLS
                     WWMDQANQLQITVDAKTDRMVLNAIFATITNANFDRKAIIALINETMAAKKKIRAIVE
                     PKLNGIQIPGAAQWEATSEEEFDKKALLIGILAETHEDIRSLKELIMYGIKGMAAYAE
                     HAWNLNHTDKDIMSFTSNALAQLLRTDISADELVALTMKTGEYCVKTMALLDKANTST
                     YGNPEITQVNIGTRNNPGILISGHDLRDLEDLLEQTKGTGVDVYTHSEMLPAHYYPKF
                     KQYENFVGNYGSSWWKQVDDFETFNGPFLFTTNCIVPPRANASYADRIYTTGASGLEG
                     AIHIADRPEGGRKDFSVIIEHAKKCQPPTQIESGSIVGGFAHAQVLALADKVVDAVKS
                     GAIRKFVVMAGCDGRMKSREYYTEFAEKLPNDTVILTAGCAKYRYNKLQLGDIGGIPR
                     VLDAGQCNDSYSLAVIALKLKEVFGLDDVNKLPIVYNIAWYEQKAVAVLLALLSLGVK
                     NIHLGPTLPAFLSPNVAKVLVENFGIGGITTVDEDLALMIG"
     gene            8941..>9375
                     /locus_tag="PKY63_10795"
     CDS             8941..>9375
                     /locus_tag="PKY63_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF01145.1"
                     /translation="MIRFCNMMIVRIVILTVFLLIFTVARAQIVSGVVKDGSGLPLPG
                     ATVMLKNTYTGVVSDENGHWQLKVQPGKNVLIISMLGYVTIEKELVVPRSGTSFEAIL
                     QNSVTQLNEALIVADTRDLAKEIMQKGISKNSKTASTLLIKAV"
BASE COUNT         2474 a         1863 c         2188 g         2850 t
ORIGIN      
        1 gaaactggac cttcgtggca ggtaacaacg cattggaaat aagttgctgc tgtttgaatt
       61 gttccatagg attcagcggt ggctccggta atattggtat aagtaatacc atcggttgag
      121 ctctgccact gataggtaac accagttcct gcagtagcat tttcaagtgt cagtaatacg
      181 ccatctccat aacacaaagc agttgaagaa gctattgtgt tgcccggagt cggagttgta
      241 caggtaaata caaccggagt caggttttca tctgctccca tatcagggaa gtatgtatca
      301 cgtgcttcac catcaatgtc ctcagtaatt tcgggaataa gcatggctgt tccgttcaat
      361 gccggattag tcgtatgcaa atcggtcaga ccgttgaaag taggatcaac agaaattgaa
      421 tgggcatcct gcgatgaggc tgtctgccag tctgcaaggg tattgcggtc agcggtaaag
      481 taccctgcat agttatttgt gcctgcagca tcaacaaaca ggtcgttata atctgaatat
      541 aatgttgctc ctgtggttct gtatatgcag taacgctttg aggttgcatc tgtggctgat
      601 gtattggcaa aaacattatt cagaacattg tgagttccac tgcttcccag atacagggcc
      661 gcggatgaag ccgcagcatc ctcggagatc agcacagaat tgtgtactat gttgctggtt
      721 ccggttccgg tcaagccgac accaatacct cttacaacaa catttgaaga cgcagtggtt
      781 attccatggt tcaggtcaga gaccatattg ttgaatatat tggccatggc acctgaattg
      841 atatcggttc ttaatccata aacataggtt gttgtccctg ttcctgtcat gttgattgag
      901 taaacgatat tgtttccgat attgttgtcg gttccaagca ggtattgagc gtaaatacca
      961 taaacggaag aactggcagt tacatttctg acaatatttc caaaaatggt tatattctgc
     1021 tggtagttgg ctctgatacc gttggcagca gagcttccgc ctccaatgtc attggcggtg
     1081 gcagcaccta caatgtttga atcaatgaca cagttaatgt caggatatgt tgaggaagtt
     1141 cctgtaagtt gaattccatg gtaacaattt tctataatat ttttatggta aatattgtag
     1201 gagtttgcac cgctggcatt tgtggctgaa atcggggtgt attgcgaaat accgcgtgct
     1261 gctgtatttg cacggttcag tgtgactttg cagtttgcaa ttgcattata ctgggcaccg
     1321 tcggttgctg atgcattggc gatataatat ccatattcaa cgtttgatcc acttgcaata
     1381 gcaatatcaa tagcattgaa cgtgatatag tcaccaccga caatggcaat accggcttct
     1441 ccggttgcag ttgttccggt cggacttata attggatttg cacctgcgcc tgccttaata
     1501 aaagtaattt tattgaccga agttccgctt gctgtaatag cagggcagtc ttcggcaaaa
     1561 gtctgtcccg cagctactgc aaaagttaca gatgcggaaa tgccattatc gttcagatag
     1621 tttatggcat ctgtgaagtt attgaagttg tctgtgccat cgtgaagcaa cacagctgtt
     1681 gttggaaaaa gattattgat ggaatatata ccgtgcaggg gtacctgagc taaggtagat
     1741 tgtgaaccag tcagatcagt acttaaaagg cctgattcca cagttgcatt gaccttccag
     1801 taataaaggg ttgagggagt cagcccgttt gcctgatagg atacggcgtt tgcaggtaaa
     1861 ttggtaacag gagtatatgt tataccatcg agtgagtttg aaatgatata gaatgtttca
     1921 tctggagaat cagtccagtt taaggtcatt tttgaagctc cgacatttgt gaatgtcaga
     1981 tttgttggag ctgttaccgt agtgggaggt gtccaggtgt aaactgttct gttgccttgt
     2041 gttgttgagc caagattagc aataagaaca ctggctgcaa aagaattggt tgttggtgaa
     2101 gcggcaggtg tgaaagtagg agttgctcca acagtcacat agccagcagt agatgcagta
     2161 tttgaagaag aaataaaaat agatctggtt acagcagagg aacttgaatt gtaaatttcg
     2221 ccgtaaataa aatctatttt tccactgcct tcatacagca gaacttgcat atcaccatct
     2281 gaggcatcag tgtaataaga cgctgcaaat tcattccatt gaaccaccag aatcctattg
     2341 ggagcggtgc caattacttt atagacgatg ccattgttca cttcattatc tccggccatc
     2401 ggagctaaaa tgggtacacc agccgaaaag gatgttatac tgtagctgct gataattggt
     2461 gatccaaggc gtaactgtcc attggagttg acactgaact gcgtataggg tgtccccata
     2521 aaataaaatg tgaagccaat gtctgtcaat gccgaggctt catcatcatt atttcccgtc
     2581 atgagattcg tggttccgct tgacatgtcc tcaagcgagc ccgtgaaaga gtaactgaac
     2641 gcgtagttcg tgatactctg cccaaacgac actccaaatg ccaatagggc aatccacatt
     2701 aatagtagtt ttttcatatt taaagatttg gttaaaaatt cgccattacc ccccaagggt
     2761 tttgagcgga tggcaaagtt aatgaattta gttttggttt cacatttgtt cacataaaaa
     2821 tcaggacaat cattgttaaa ttactttgat tacttcaggt ttaacatgta ttattgtata
     2881 aatagtctat tttaatttta tgtatatcga aaaatctgga ttttttttta acagattgtt
     2941 gaatacttga aattttattg aatttcttta tccgcaaaca aaaaagcgca tcaaatcatt
     3001 tgatttaatg cgcttttcat ttcagaagct tatttatttc agcttaactt cctctgtctg
     3061 aattatctcc agtgttgctg catcataaat aaaaacgaca atgttcatgt gatctttgtt
     3121 ccacgaagca tttgatacag tgtaggaaag tgttttgcta agctgtgtgc caacagtggc
     3181 tccgcccgaa caaagtgtgt cgccccatgt tccgttcatc gatccgcgga gtacgtgcat
     3241 gtgaacataa tctgcaattt ccggggtagt gcctacagcc ggatcattgt ttttctgata
     3301 atccacaatg ctgtcttcgg tcagataggc cgaaatcatg atgggattgg aaaagtcggc
     3361 caaaacatca acctttgcag tcaggtcaat ggttgaagtt gttgtattga atgtgtgggt
     3421 cgtattgatg tcggcttcgg gtgccagcat cagcagttct gcaattttcg atgcccaatc
     3481 gtttggaccg ataattctcg agccgctgta ttccttgcgg ttgatggtgc cgtttggatt
     3541 tccaatcaga tcaacaccga aatatgtatt gaggtcattg ccagttgtgc atctgaaatc
     3601 ggtcatgtaa tttggtgatg ccagattggc aaaaagtcct gcatgtatgg taatgagcga
     3661 tatttttccg ggatacagtt ccattagttc gtgggctatt atggctgctc ccggacagtt
     3721 tggacattga tggccggtgt agtcttcaag cagaactttc cgaaccacgg tgtctgtaac
     3781 cacaggattg ttattcgttt gtgtataggg gggctcaata acatcacagg aagtgaaaat
     3841 tgctattgcg gcaatgatgc agatttgaaa caactttttc atcacagatc aaaaattaaa
     3901 agttactggt aatggtaaca aggaaaccat aggaggcggg cacattgcgg cagacaccgc
     3961 caacacagac aattccttcg cgctgtttcc cataagtaat ggcgatgcgg ctgctgtttt
     4021 tggtataacc gaagccggtt gtgtagtaat gcagcttttt atcatcagca ggatttccgt
     4081 aattatagct gtcaccggcc atgaagaacc attttggcgc gagggtgaat tcaatcatgg
     4141 cattggccca gtttttttca tcctgtttgg tcatcagatg ttgcacctcc atgcggagcg
     4201 attttgttgg tgtgatttta taggtgacat cggcaactcc aatatggctg tagaaggtgc
     4261 catcggtatg accttcaatt atatcgctgt tgtaggtgag ataggcccag gtgaaaattc
     4321 ccttgacttt tttgctgaat ttgtggctga tttcaatatt gatatcttca aaatacaatt
     4381 catcgccaat ggcaaaaaag tcgcttgtat agccaagagt ccctgattgc atcagtgccg
     4441 tagagtcgtt tattttttgc atatcaatgg cattggctct tgaataattt actgcgacgt
     4501 cggttccgta atggccgccg agtttagtac ctttttttat tttgtagttg agttgtccct
     4561 gaaaagccat ttcaccgttg ggttgagagg catagggata gaatgccagc agggtgtatg
     4621 agtgaggttt ggttaatgcg ggcagataat tcatcgtgag tacattgcct gttgctgagc
     4681 ggtctgagcg aaaattcatg ttgtctattc ttttcgcact caaggtaata ccaaaacctt
     4741 tttttgaata actgagtgat gtaaaaaggg cttcacctgg tttgtaaatc attttattta
     4801 ccgtggaggg gtcattgact ttatgggcat attcaccgtt gagcatccag ttgccataaa
     4861 tcagtgtagt gcggacagcc catcctccaa cgttttgggg gagattgtaa atcgggtcgc
     4921 ggtcggcctg atatttactc acaaaactcc cgccgacagt tactttcagt ttcgatgccg
     4981 cccatttatt gttgaattca ttcagattga tttcgccatc aaggccgcga acaatgcctt
     5041 cgcctttgtc gaagaaaaag cgttgctgtc cgatagcgcc tttcaggtaa atacctttgt
     5101 aaggtttgta tctgatccgg attccgtcaa gcgaattatc aattccaaga cttcgttctt
     5161 cgtaactgcg aagcagaagt cccgatccga attgctcgta gtaatttcct gcggtaattt
     5221 caaaatcttt gtgcgaataa gatgcaaaac gatatgggat accactgcct ttgtagcgcg
     5281 ggtcgaatcc ttgcatcaca ttgttgtaat tttcatatct tactccggca gaaaaattcc
     5341 ctgatgtaaa aagcagattg aggtatccgt tgctcagcag cttttcagga acagtatcgg
     5401 caccaatcga ggagtcaggc aaataatatt gaaagtcggt ctgaaagtcg ccgtgaattt
     5461 gtgcattctg caatacattc tgagcagcac ctgctattga actcagtata aatgctgctg
     5521 caaaaaattt cttcatgtgt ttcgccataa actttattcg ctgatttttt cgccattgtt
     5581 gagtttgcgg atcagttcaa tcagttccag ctcgccgcct tcggtaaacg aagtgtgctg
     5641 ccagacaatg tttctgtctt tgtcaatgat aaaagtatgc ggtaccagat tgacattcat
     5701 ggcgcgcttg aaatcgaagt tcggatcgca gaaaacatcg aaaggccagt cgcgcccatt
     5761 gacaaacgag gccacattgg gaaatgttct ggagtcgtca atgctgatgg catagagttt
     5821 tactcctgtt tcttcctgcc attcttcata aacttcagca aaggtggtga gttcttttac
     5881 gcagggcttg caccaggtgg cccagaagct gataatgatg ggtttgcctt gatttaagat
     5941 agaatcagat gatactatcg tgccgtctgg tttttttaaa tcgaccgatg gcaaagtgtt
     6001 gttctgtgcg gtcatggcta tgacagcaaa aaaagcaatg attacgagaa ttgatttcat
     6061 tgaagtggtt ttggtgtaag cgcttctcaa atttcttacc aaaaaaaggt ttttgattga
     6121 atagtgcaaa tgtaattgaa tattttattg aaaaaaatac atatgttcat aagcatgttg
     6181 tcagaactgt tgtttttaat tgaaaatgaa acagatacgg tgtgaaatat aaaaatctgc
     6241 agccgtgttt tacattaacg gaggcagatt ttatttgaac ggacaaacat tttgaggaaa
     6301 aactatccct taatcataaa tagcagcatt ttgaatgctt caggtgcttt aactgcatgt
     6361 ggaatattgg ccggcatgat gataagctcg ccggctttga cggtatgagg tacaccgccg
     6421 ataatgtatt cgccggttcc ttcaatcacc tgaaccactg catcgaaagg ggctgcatgt
     6481 tcgctcagtg cctgtccggc atcaaatgaa aaaagagtca gtgatccgcc ggtgtttttc
     6541 gatatgattt tactaacgat tccaccaggc gtataatcaa tggcggcagc tattgttgta
     6601 gcttttgagt gttcaaatgc gttttccatg gctgtttatt ttgggggtgt tttttgttgc
     6661 gttttatttc ataagatgga aggatgctgc ctttatgcac attgcttatg gctttggcgc
     6721 ttttacaacc aaaacacgaa caatattgtc cgattgattc gataagaaat gcacaatgtc
     6781 ttttggactt tcaatgagcg ttcctgctgt aaaggattct gtttcttctc cgatatgcac
     6841 agtggctgtt ccttcaaata caaagaagaa tacatcgacc ggggtggtgt gtggtttcag
     6901 cgcctgaccg ggctgcagac taatcatgag cgcctgagcg gtcggatcgt tatacaacat
     6961 ttttacatcg acaccgtgcg ggttttcttt tgctccggca gttgaataat gggtgctttt
     7021 catatagaat gatattaaag aaaagggaca ttcagcatgt cccttttctg aattttaatt
     7081 aaccaatcat caaggcaagg tcttcatcta cagtggtaat gccaccgatc ccgaagttct
     7141 caacgagcac tttggctacg tttggcgaca gaaatgccgg gagcgttggt ccgaggtgga
     7201 tgtttttcac gccgagtgaa agcagcgcca ataaaacggc aacagctttc tgctcgtacc
     7261 atgcaatgtt atacacaata ggcaatttgt tcacatcgtc gagaccgaac acttctttca
     7321 gtttgagtgc gattacggcg agtgaatagc tgtcgttgca ctgaccggca tcgagtacgc
     7381 gtggaatgcc gccaatgtcg cctaattgaa gtttgttgta gcggtatttg gcacaacctg
     7441 ctgtaagaat aactgtgtcg tttggcagtt tttcggcaaa ttcggtgtag tattcgcgcg
     7501 atttcatacg tccgtcgcaa ccggccatca ccacaaattt gcggatggcg ccggatttca
     7561 ctgcatcgac cactttatcg gccaaagcca gtacctgagc atgagcaaat ccaccaacga
     7621 tgctgccgct ttcgatttgt gtcggtggct gacatttttt ggcatgttca attatgacgg
     7681 aaaagtcttt tcggccacct tcgggacgat cggcaatatg aatggcccct tcgagacccg
     7741 atgcgccggt ggtataaatg cggtcggcgt agcttgcgtt ggcgcgtgga ggaacaatgc
     7801 agttggtggt aaagaggaat ggtccgttga aggtttcaaa atcatcgacc tgtttccacc
     7861 acgaagaacc gtagtttccg acaaaatttt cgtattgttt gaatttggga tagtaatgag
     7921 cgggtagcat ttcgctgtga gtgtaaacat caactccggt acctttggtt tgttcaagca
     7981 aatcttcgag gtcgcgcagg tcgtggccac tgataagaat tcccggattg ttgcgcgttc
     8041 caatgtttac ctgagtgatt tcaggattgc cataggtgct ggtgtttgct ttgtcgagca
     8101 aagccatggt ttttacgcag tattcaccgg ttttcatggt cagggctacc agttcgtcag
     8161 cactgatatc tgttcgcaga agctgagcaa gagcattcga agtgaagctc ataatgtctt
     8221 tgtcggtgtg gttcagattc catgcgtgct cggcataggc agccatgcct ttgattccat
     8281 acataatcag ctctttgagt gagcggatgt cttcgtgggt ttcggccaga atgccaatca
     8341 aaagtgcttt tttgtcaaat tcttcttcgc tggttgcttc ccattgggct gctcccggaa
     8401 tctgtatccc gttgagtttg ggctcgacaa tggcgcggat tttcttcttg gccgccatgg
     8461 tttcattaat caaagcaata atggctttgc ggtcgaaatt tgcattggtg atggtggcga
     8521 atatggcatt cagcaccatg cggtcggttt ttgcatctac tgtaatctga agttgattgg
     8581 cctgatccat ccaccagcta agccctttta cattatatat taaaaggtcc tgcatggcag
     8641 aaacgggctc tttctttccg catacaccgg caatggtgca gccggttcct ttggcggttt
     8701 cctgacactg aaaacaaaac atgctcatag tttgtttggg ttttaaatta gcaattaggt
     8761 aaaacaatac gcaaatgtat aaatgaattt aacgccaata atacattaac aataaattaa
     8821 cgactgattg tatcttcttc attgaaaatc aggcattttt caacatcaaa tatacgaaat
     8881 acggatgcta tttgtaacta aatgcatgta aaattcgtca aaggaaacat aaaaccacca
     8941 atgattcgtt tttgcaacat gatgattgtc cggattgtaa tactgaccgt gtttttattg
     9001 atttttacgg ttgccagagc tcagattgta agcggggttg taaaggatgg atcggggctt
     9061 ccattgccag gagccacagt aatgttgaaa aacacctata ccggtgttgt ttcggatgaa
     9121 aacggacatt ggcaactaaa agttcagccg ggtaaaaacg tgttgattat ttcaatgctt
     9181 ggatatgtta ccattgaaaa agagcttgtg gtacctcgtt ccggaaccag tttcgaagct
     9241 attctccaga attcggtgac tcagttgaac gaagcgctta ttgtggccga cacgcgcgat
     9301 ctggctaaag aaattatgca aaagggcatc tctaaaaact caaaaaccgc atcaacactg
     9361 ttgataaagg ctgtt
//
LOCUS       DAOPKE010000102         9318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7789_length_9318_cov_12.343841, whole genome shotgun sequence.
ACCESSION   DAOPKE010000102 DAOPKE010000000
VERSION     DAOPKE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7789_length_9318_cov_12.343841"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKY63_10800"
     CDS             complement(<1..1213)
                     /locus_tag="PKY63_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01146.1"
                     /translation="MKKIIILLLCVASYTQVCPQKLIQKKTYWDIWETQLSLVWYETG
                     DGIQHGEQKAYYQDGTLMSYKKYNMGTCVHSTYYDAAGNITRVGNWDNDGNIEGEQKE
                     YEYSEGETFLQRSIFCKDKEVISYSSYYGKDRPKIQYEKNKFLRTYDNNNVKTSDLSL
                     INGEYNGFLTAYEGEYYFEKSVLVKYWNCTHNGMQHIDFVPDNKVEGTFLVATKYSGI
                     ANKTAIKICRYKNLEKTKPLLKKINISFPDGNFETEPYLYEIEEKFNNNMQITSTLLD
                     TNVECDNVSSLKDKCLEKYLKYSNGQKKFSGDTTFYESGKIKSLGNKLEYYESGIIKR
                     NGDSLYFENGKMQTDGINTWFENGNPKRKLLTDRTEFYYESGKIKQMVLIDFSKYIDY
                     YENGNTSKIWEK"
     gene            complement(1331..2194)
                     /locus_tag="PKY63_10805"
     CDS             complement(1331..2194)
                     /locus_tag="PKY63_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01147.1"
                     /translation="MKISKKTIWIISVIAAIVIISGISKLCSSSNNNGEIEISNFKVK
                     PISTEIEGGLKGYLSIVDGTYEGTSPDFGQCFIKIKIKVLKTWSEVDKSKADDIDDYS
                     ADLKLTVLDENGIPISGFEEYTYSSSSDNDSELHNALKKGTGDLILNFASLMYDSEEL
                     AELFTKAAKFQVSGDMNQYRFKAAPGEESTDNQSESSGANSSTVSKSDCESFINGYEK
                     FASNYLAFSKKYKKDPNDPTLISDAAEMNAETLKWSSKLESYGGCDDPSYIQRLAAVQ
                     ATLAKAAANLY"
     gene            complement(2323..2568)
                     /locus_tag="PKY63_10810"
     CDS             complement(2323..2568)
                     /locus_tag="PKY63_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01148.1"
                     /translation="MKKFKSNLVMFLIFVAYFAIVILILVKTFPDSKIALIIGIPISF
                     IYAIVCFFNKKTRSVMTIWWGILSLLSCAWWIYLMSK"
     gene            2734..3000
                     /locus_tag="PKY63_10815"
     CDS             2734..3000
                     /locus_tag="PKY63_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF01149.1"
                     /translation="MKRKNKRKLIFDAAGLAAFGSRLKYLRAEAGFTQAQLAFEAGVS
                     LSQIARIETARINPTLSTVFTISRALDIPLSVMFDFDLPDNKRT"
     gene            3104..3655
                     /locus_tag="PKY63_10820"
     CDS             3104..3655
                     /locus_tag="PKY63_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01150.1"
                     /translation="MLSAIIKKEVEDTFGKEIRYPADCEALSAHISDKTGQSISITTL
                     KRMFGFVNGTHEPRLYTLDVLAQYLGYPNWDVYYEKFIHIENSEFFTAEQIEIDALKP
                     DAVIEIIYDPDRIIRLVYQGNFRFNVEHSTNSKLLRGDQLKIMSIVKHYPLIIASVIR
                     DGKDLGRFVAGKSSGITGICIKS"
     gene            3660..4970
                     /locus_tag="PKY63_10825"
     CDS             3660..4970
                     /locus_tag="PKY63_10825"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPF01151.1"
                     /translation="MPEIENSGFDNSGENFLNAGNFSAAELLPIQSNGSVVYKIRIKD
                     RWLLLKRISLEFRNNPLYIAALEKEFNLGFSLDHPNIVKYLNKGSDKDGLYILAEFID
                     GITLRSLIQKNANGIGDKKLIEKIVRQLLDALDYLHSNNILHLDLKPENILITYKGNN
                     VKLIDFGMSVSDSYISISSGTRKYCSPEQLSNPECATSGNDFFSLGLVILEMFTGSTD
                     LSGVGKVPRRYRKIVRKCLDSKKENRFQSAQDILRKLNERKTKMTIFSVVSLALVATI
                     AVLILFFITNRNHIRNEKTINTPLINANNPSLVKNEQTELNSNVIYLDSNYKTDKSSE
                     LMKVVSTPLPVDDSIRMCRLGINLFSEFLKRVEAYDKNPGLRSRKLVLIEIKGECIED
                     FNDSVTIALSRYSIGSPLHSRLLDLFTKHELQSEDMIDHYVYGN"
     gene            complement(5222..5737)
                     /gene="mreD"
                     /locus_tag="PKY63_10830"
     CDS             complement(5222..5737)
                     /gene="mreD"
                     /locus_tag="PKY63_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPF01152.1"
                     /translation="MNNKLILTNIFRFLVVILLQVTIFNYTNLFGYINPFIYLYFVLL
                     IPFETPMWIALILAFLTGFTQDLFVSTGGLHAAATTAIAYLRPVLLRTIVSKREYESG
                     LQPGVSDLGWKWFLTYAGVSILIHSLLVFYLDVFRFSHFFTTFKHALFQALATLFFIA
                     LFEFVVNIRKK"
     gene            complement(5727..6566)
                     /gene="mreC"
                     /locus_tag="PKY63_10835"
     CDS             complement(5727..6566)
                     /gene="mreC"
                     /locus_tag="PKY63_10835"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPF01153.1"
                     /translation="MRNLLDFIKSIRILLLFLFLETMALLFTFNESAFHGAASFQFFT
                     GINNRIMAVRSDVVSFFNLRDENRKLVEENMRLREQLKESYIISDQRVFTVNDTLYRQ
                     EFEYLTASVINYTISYQDNYITLDKGSQQGIEEGMGVYSPAGVVGIVDHVSKNYCLVR
                     SVLHSEVTVSTTIKDKGVTGTCVWDGLNYAYGVLKDIPVHVKLKQGDTLLTSNYSSIF
                     PAGIPVGYVTEFSTDATRAFYNVRYRIAADFGNLSSVYIIRNLIKGELEELEKLKEDK
                     NEQ"
     gene            complement(6580..7602)
                     /locus_tag="PKY63_10840"
     CDS             complement(6580..7602)
                     /locus_tag="PKY63_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPF01154.1"
                     /translation="MGLFNFMTKEIAMDLGTANTVIIHNDKVVVDEPSIVAIDRTTNK
                     IMAVGKRAMMMHGKTHENIKTIRPLRDGVIADFMIAEHMMRELIKMINTRKPLFPPSL
                     RMVISIPSGITEVEERAVRDSAEQAGAKEVRLIHEPMAAAIGIGLDVLEPSGNMIIDI
                     GGGTSEIAVIALGGIVSNKSIRIAGDDFNADIEEYMRKAHNLSIGERTAERVKIEVGA
                     AMAEIENVPPDFPVHGRHLLSGIPREVMVSHAEIAQALDKTISKIEAAILNALENTPP
                     ELSADILNTGIYMAGGGSLLRGLDKRIEQKTKLKVHVAEDPLRAVARGAGIALKNFDK
                     FPFLIK"
     gene            complement(7620..9140)
                     /gene="purH"
                     /locus_tag="PKY63_10845"
     CDS             complement(7620..9140)
                     /gene="purH"
                     /locus_tag="PKY63_10845"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPF01155.1"
                     /translation="MSSGKKISSALISVYDKTGLEPIIHRLNELGVTLYSTGGTADFI
                     QKLGVKVIDISELTGYPPVFHGRVKTLHPAVFGGILYRRDNSEDLAQAKEFNIRAIDL
                     VVVDLYPFEETLKKTSEEAQLIEKIDIGGISLIRAGAKNFNDVLIVPSVEYYGDLLDI
                     LQSGETTREQRRLFATRSFEVSSHYDSQIFTWMNGNHEVDAMKLSLRNGSQLRYGENP
                     AQKARFYGNMDEYLTKLNGKELSYNNLQDIGGALDLLGEFDDCACVIVKHTNACGVAE
                     AGTVHEAWTKALSCDPVSAFGGIVAFNKKLDAETCAKLHEFFFEVLIAPDFDEAGLAQ
                     LTSKKNRIIVRLNKLPQATSMIKQLSFGCLWQETDSLKENLQQWELKAGNAPAQAVID
                     NMLFGMKVVKHLKSNAIVLVKDRLLIGSGTGQTNRVDAVKQSVEKAIKFGFDVKDSTL
                     CSDAFFPFSDSAEIAYNAGVTAIAEPGGSIRDEETVKYCTEKNITLMFTGNRHFKH"
BASE COUNT         2611 a         1872 c         1938 g         2897 t
ORIGIN      
        1 ttttttccca aatttttgaa gtgtttccgt tttcataata atcaatatat ttactaaaat
       61 caatcagtac catctgtttg atttttccag actcataata gaattctgtt cgatcagtga
      121 gtagctttct tttcgggttc ccattttcaa accaggtgtt tattccatcg gtttgcattt
      181 taccgttttc aaaatataat gagtctccat ttcgtttaat aatacctgat tcatagtact
      241 ctaatttatt tccgagcgat ttgattttcc cggattcgta aaaagttgta tcgccactaa
      301 atttcttttg accattgctg tattttaaat acttttcaag acacttatcc tttaatgaag
      361 aaacattatc gcattcaaca tttgtatcta aaagagtgga tgtaatttgc atattattat
      421 taaacttttc ttcaatttca tataaataag gctctgtttc aaaattccca tcaggaaatg
      481 aaatgttgat tttctttaac aaaggttttg ttttctccaa attcttgtat ctgcatattt
      541 taatggctgt tttatttgcg atacctgaat atttcgtagc aaccaaaaat gttccttcaa
      601 ctttattgtc aggaacaaaa tcaatgtgct gcattccgtt atgtgtacag ttccagtatt
      661 tcaccaaaac actcttttca aaataatatt ctccttcgta agctgtaaga aaaccgttgt
      721 attccccgtt tattaatgac aagtcgctgg ttttcacatt attattgtcg taggtgcgta
      781 aaaacttatt cttttcatat tgaatctttg gcctatcctt gccataatat gatgagtagc
      841 ttattacctc tttatcttta cagaatatac ttctttgcag aaaagtctca ccttcactat
      901 actcatactc tttctgttct ccttcaatat ttccatcatt gtcccaattt ccaactcgtg
      961 taatattccc ggctgcatca taatatgtac tatgaacgca cgttcccata ttatattttt
     1021 tgtaagacat tagagtcccg tcttggtaat aggccttttg ctcaccatgt tgaatgccat
     1081 caccggtttc ataccagact aaacttagtt gtgtctccca tatatcccaa tatgttttct
     1141 tttgaattaa tttctgagga cacacttgcg tatatgaagc aacgcaaaga agcagaatga
     1201 ttatcttttt catattgtga ttgtttttgt tgttttaaga agtaaaaaga gtggggattt
     1261 cttaataaac gcccactctt tctctaaaat gcatacagct taaaccgcat aattacaatg
     1321 gtggttttta tcagtacaaa tttgcggcag cttttgcaag tgttgcctga acagctgcga
     1381 ggcgctgaat gtatgaaggg tcatcgcatc caccataact ttctaacttc gatgaccatt
     1441 ttagtgtttc agcattcatt tctgcagcat cgctaatcag tgtcggatcg tttgggtctt
     1501 tcttgtattt ctttgaaaaa gccaggtaat tgcttgcaaa tttctcataa ccatttataa
     1561 atgattcgca atcactcttg cttacagtcg aactatttgc accgctgctt tcactttgat
     1621 tatctgtcga ttcttcccct ggggctgctt tgaatctgta ctgattcatg tctccggaaa
     1681 cctgaaattt agcagctttt gtaaataatt ccgccaattc ttcgctgtca tacataagac
     1741 tggcaaaatt taggattaag tcaccagttc ctttttttaa tgcgttatga agttcactgt
     1801 cattatctga gctgctgctg tatgtatatt cttcaaatcc cgaaattgga atgccgtttt
     1861 catctaaaac ggttaacttc aggtctgctg aataatcatc aatatcgtca gctttcgatt
     1921 tatcaacttc actccatgtt tttaaaactt ttattttaat tttgatgaag cattgaccaa
     1981 agtctggtga ggtcccttcg taagtgccat caacaattga caggtatcct ttaagtccac
     2041 cttcaatttc tgtgcttata ggctttactt tgaagtttga tatttcaatt tcgccattgt
     2101 tattagagct gctgcataat ttagaaattc ctgatataat tacaattgct gcaattacac
     2161 taataatcca aatcgttttt tttgatattt tcatgaggta aaatttttaa ttcctactca
     2221 tttggctttt cggtatacgc cccgtttttg aatggactac ggaatccata tgtttaattt
     2281 gtatcagttg ctgtattatt gttattgcgg acacctgata tcttattttg acatcaaata
     2341 tatccaccat gcgcaagaga gtaaactcaa aataccccac caaatagtca ttacggaacg
     2401 tgttttttta ttgaaaaaac acacaattgc gtaaataaat gaaattggaa tgccaattat
     2461 taaagctatt ttgctatcag ggaatgtttt gactaagatt aaaataacta tggcaaaata
     2521 ggccacaaaa attaaaaaca taaccaggtt acttttgaat tttttcatga tgttaaattt
     2581 tattcctact cataaggctt ttcggtatcc gcctccggtt tttctggtac ccggactacc
     2641 tttttgattg tttctccagc aaatataaaa gaaattttct aaaaaaagca catgtgctat
     2701 aattattttt ccataacacg gtaattttca tccgtgaaac ggaaaaacaa acggaaactt
     2761 atttttgatg ctgccggctt ggctgcgttt ggatcgcgat tgaaatatct gagagctgaa
     2821 gccgggttca cgcaggcgca actggctttt gaggccggtg tttcgctgag ccagattgcc
     2881 cgtatcgaaa ctgcgcgcat caatccgaca ctcagcacag tattcaccat ctcacgggct
     2941 ttggatattc ctttgtctgt catgttcgat tttgatttac cggataacaa aagaacataa
     3001 aattcattaa gactatatcg ttcaaattgt tcaattagtt cactttgttc attttagaat
     3061 ctaatgaaat ttccaatata tttgcgtttc ctatacaacg cacatgcttt cagctattat
     3121 taaaaaggaa gttgaagata cattcggtaa ggaaatccgt tacccggccg attgtgaggc
     3181 actatctgct catatctcag acaaaaccgg tcagagcatt agcattacca cactgaagcg
     3241 catgtttggc tttgtgaatg gcacacatga accaaggcta tacacgctcg atgtattggc
     3301 gcaatattta ggatatccga actgggatgt gtattatgaa aaattcattc atattgaaaa
     3361 ttccgaattt tttactgcag agcaaataga aattgacgct ctgaagccgg atgcagtcat
     3421 tgaaatcatt tatgacccgg atcggattat acgattggtt tatcagggaa attttcggtt
     3481 caatgttgag cattcaacca acagcaaact tttaagagga gatcagttga aaattatgag
     3541 cattgtaaaa cattatcctc tgatcattgc gtcggtcatc agagatggaa aagatctcgg
     3601 aaggtttgtt gccggtaaat caagtggcat aactggcatt tgtattaaaa gttgatgata
     3661 tgccggaaat agagaactct ggtttcgata attccgggga gaattttctc aatgcaggaa
     3721 atttctctgc tgcagaatta ttacccattc agagcaatgg cagcgtagtg tacaagatcc
     3781 ggataaagga cagatggctg cttttgaaaa gaatttctct ggaattccga aacaacccat
     3841 tgtatattgc tgcacttgaa aaggaattca atcttggatt ttcgctcgat catccgaata
     3901 ttgtaaaata tctgaacaaa ggcagtgata aagatggttt gtatatactg gcagaattca
     3961 ttgatggtat tacgttacgc agccttattc agaaaaatgc aaatggaatt ggtgataaaa
     4021 aactcattga aaaaattgta cggcaattgt tagatgcgct tgattacctg cattccaaca
     4081 atattcttca tctggattta aaacccgaaa acattctcat tacctataaa gggaacaatg
     4141 taaaactgat tgattttgga atgtcggttt ccgattcgta catttcaatt tcgtcgggaa
     4201 cgaggaaata ttgttcacca gagcaattga gcaatccgga atgtgcaact tccggcaatg
     4261 attttttctc attgggtttg gttattcttg aaatgttcac cggaagcact gatttatcgg
     4321 gagtcggcaa agttccacgc aggtatcgta aaattgtccg caaatgtctt gattcaaaaa
     4381 aggaaaacag atttcaatcg gctcaggata ttcttcggaa actgaatgag agaaaaacga
     4441 aaatgacaat cttttcagtt gtgagtttgg ctttggtagc gacaattgct gttcttattc
     4501 tcttttttat aactaataga aatcacatca gaaatgaaaa aaccatcaat acgccattga
     4561 taaacgcaaa taatccatcg ttggtaaaaa atgaacaaac tgaattaaat tcaaatgtca
     4621 tttatttgga tagtaattat aaaactgaca aatcatcaga attgatgaaa gtggtatcaa
     4681 ctcctcttcc tgtagatgat tcaataagaa tgtgcaggct gggcatcaat ttgttctctg
     4741 agtttttgaa acgggtggaa gcatacgaca agaatccggg cttaagaagc cggaaacttg
     4801 tattgataga aattaaagga gagtgtattg aagattttaa tgacagtgtt acaatcgctt
     4861 tatcccgtta tagtattgga tccccactgc attcaaggct attggattta ttcacgaaac
     4921 atgaacttca gagtgaggat atgattgatc attacgttta tggaaattaa gggatgaaat
     4981 tgttgttgtt tttaaattca attttttagc gtatatatgt tgtaaaagac ataggctgta
     5041 cacaataaat caacagaaga aaagtctgtt ctcttaggag agatcccggt ttggcagtga
     5101 atgctgaaac tcaacaagtg accgggaact caacactttt ttttctgttt cccaaaagta
     5161 tatacaaagg tgtgttaccc tcaatatttt ttagaacgac aaaatatgtt tcagaatgtc
     5221 atcacttctt cctaatattc acaacaaact caaaaagtgc gatgaagaac aatgttgcga
     5281 gcgcctgaaa caatgcgtgt ttgaaggtgg tgaagaaatg actgaagcga aatacatcga
     5341 gataaaaaac aaggagactg tgaatgagaa tcgatacacc tgcgtaggtg agaaaccact
     5401 tccaaccgag gtcgctcaca ccgggctgca gacctgattc gtactctctt ttggaaacaa
     5461 tggtgcggag cagaaccggt ctcagataag caatagccgt tgtggcagcg gcatgcagac
     5521 cgccggtact gacaaacagg tcctgtgtaa aacccgtgag aaaagcgaga atcaatgcga
     5581 tccacatggg tgtttcaaat ggaatcagca gaacaaaata aagatatata aatggattga
     5641 tgtaaccgaa aagattcgtg taattgaata ttgtcacctg aagcagaatg actaccagaa
     5701 agcggaagat attggtaaga atcagcttat tgttcattct tatcctcctt cagtttttcg
     5761 agttcttcca gctcgccttt gatcaggttg cgaatgatgt ataccgaaga caggttgcca
     5821 aaatcagcag caatgcgata ccggacattg taaaaagcgc gtgtggcatc ggtgctgaat
     5881 tcagtgacat aacctaccgg tattcccgca gggaaaatac tgctgtaatt gcttgtaagc
     5941 agcgtgtcgc cctgttttag tttcacatga acgggaatat ctttaagcac accataggcg
     6001 taattcaagc catcccagac gcaggttccg gtaacgcctt tgtccttgat cgttgtactg
     6061 actgtcactt cggaatgcag aacagatcgg acaaggcaat aattttttga aacatgatcg
     6121 acaataccaa ctacaccggc aggcgagtaa acgcccatgc cttcttcaat tccctgttgc
     6181 gatcctttgt ccagcgtgat gtagttgtcc tgataactga tggtataatt gattacactc
     6241 gcggtgaggt attcaaattc ctgccggtac agcgtgtcat ttacagtaaa aacgcgctga
     6301 tctgaaatga tgtaggactc tttgagttgt tcgcgcagtc tcatgttttc ttcaaccagt
     6361 ttacggtttt catcacgcag attgaagaat gagaccacat cggagcgaac ggccataatg
     6421 cggttgttga ttcccgtgaa aaactgaaac gaagcggctc cgtgaaaagc actttcattg
     6481 aaagtgaaga gcagcgccat tgtttcgaga aacaggaaca gcaacagaat gcgaatactc
     6541 tttatgaagt ccagaagatt tcgcataaag cgctttcgct tatttaatca ggaacggaaa
     6601 tttatcgaag tttttgagag cgataccggc accgcgtgca acagcgcgca gcggatcttc
     6661 ggccacatgt accttcagtt ttgttttctg ttcaattcgt ttgtcgagac cacgcaggag
     6721 cgagccacca ccagccatgt aaataccggt gttgagaatg tcggcagaaa gttcaggcgg
     6781 tgtattttcg agggcgttca gaattgccgc ttcaatctta ctgatggtct tgtcaagtgc
     6841 ctgtgcaatt tcggcgtgcg agaccataac ttcgcgcgga atacctgaca gcaggtgacg
     6901 tccgtgaaca gggaagtcgg gcgggacatt ttcaatctca gccatggctg cacccacttc
     6961 aatctttacg cgctctgcgg ttctttcacc gatgctgagg ttgtgtgcct tccgcatgta
     7021 ttcttcgatg tcggcattga aatcatcacc ggcaatgcgg atgcttttgt tgctcacaat
     7081 tccgccaagc gcgataacag cgatttcgct ggtaccgcca ccgatgtcaa ttatcatgtt
     7141 ccctgatggc tcaagaacat cgagtccgat accgatggca gctgccatgg gctcgtgaat
     7201 cagacgtact tctttagctc cggcctgttc ggcagagtca cgtacggcgc gttcttccac
     7261 ttctgtgatg ccgctcggga tactgataac cattcgcaat gaaggcggga acagcggttt
     7321 gcgtgtattg atcatcttga tcaactcgcg catcatgtgt tcggcaatca tgaaatctgc
     7381 aataacaccg tcgcgcagcg ggcggatggt tttaatgttt tcgtgtgttt ttccgtgcat
     7441 catcatggcc cgttttccca cggccatgat cttgttggtg gtacggtcaa tagcaacaat
     7501 tgacggttca tcgaccacta ccttgtcatt gtggataatg acggtgttgg ccgttccgag
     7561 gtccattgct atttctttcg tcatgaaatt gaaaagtccc atagtcctgt taaggtattt
     7621 taatgtttga aatgtcggtt accggtaaac atcagagtga tgtttttttc ggtgcaatat
     7681 tttacagttt cttcatcgcg gattgagcct cctggttcgg caatggcagt tacacctgca
     7741 ttgtatgcga tttcagcgct gtcgctgaaa ggaaagaatg cgtcggaaca cagtgtgctg
     7801 tctttaacgt caaagccgaa tttaatggct ttttcgaccg attgttttac ggcatccacg
     7861 cggttggtct ggcctgtgcc gctgccgata agcaaacggt ctttcaccag cacgatggca
     7921 tttgatttca gatgcttcac cactttcatt ccgaagagca tgttgtcaat cacagcctgg
     7981 gctggcgcat tcccagcttt cagctcccat tgctgcaggt tttctttcag gctgtcggtt
     8041 tcctgccaga ggcagccgaa cgaaagttgt ttgatcatcg atgttgcctg gggtaattta
     8101 ttcaggcgga caattatacg gtttttcttc gatgtaagtt gcgccagacc ggcttcgtcg
     8161 aaatcggggg caatcagcac ttcgaagaaa aattcatgca gcttcgcgca ggtctctgcg
     8221 tcaagttttt tattgaaagc tacaatgcca ccaaatgcag aaacaggatc gcagctgagg
     8281 gctttggtcc aggcttcgtg tacagtgccg gcttcggcaa caccgcaggc attggtgtgt
     8341 ttcacaatca cacaggcgca gtcgtcgaat tcgcccaaaa gatcaagcgc tccgccaatg
     8401 tcctgcagat tgttgtatga aagttctttt ccgttcagtt ttgtgagata ttcatccata
     8461 ttgccgtaaa aacgggcttt ctgtgcggga ttttcaccgt agcggagttg cgatccgttg
     8521 cggagactga gtttcatagc gtccacttcg tggttgccgt tcatccaggt gaaaatctgt
     8581 gagtcgtagt gcgacgacac ttcgaaggag cgtgtggcaa agagacggcg ctgttcgcgt
     8641 gtggtttcgc cgctttgcag gatatcgagc agatcgccgt aatattcaac tgaaggcacg
     8701 atcaaaacat cgttgaaatt tttcgcaccg gcgcggataa ggctgatgcc gccaatgtcg
     8761 attttctcaa tcagctgggc ttcttcgctg gtctttttta gggtttcttc gaagggatat
     8821 aaatctacca cgacgagatc aatcgcgcgg atgttgaatt cctttgcctg agccagatct
     8881 tcgctgttgt cgcggcgata tagaatacct ccgaacacgg cagggtgcag ggttttcacg
     8941 cggccatgaa atacgggcgg ataaccggtg agttcggaaa tatcgatgac tttaacgccc
     9001 agtttctgga tgaaatcggc ggtgccgccg gtggagtaca gggttacgcc gagttcgttc
     9061 agtcggtgaa taatgggttc cagtcccgtt ttgtcgtaaa cggaaatcag ggctgagctt
     9121 atttttttgc cggatgacat gaaatcagtg agttagtgac tgtaaaatca gggttgcaaa
     9181 ggtacggtaa aataccttat aaaaaagaaa tttttggtgc agcttttata tccggcaatg
     9241 gcaaaggaaa tttgccacaa tggcacgaag acacaaaaag aattttgaac aaggaacatg
     9301 gaaatttgaa gtaagaag
//
LOCUS       DAOPKE010000103         9316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7791_length_9316_cov_10.576072, whole genome shotgun sequence.
ACCESSION   DAOPKE010000103 DAOPKE010000000
VERSION     DAOPKE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7791_length_9316_cov_10.576072"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKY63_10850"
     CDS             complement(<1..1277)
                     /locus_tag="PKY63_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01156.1"
                     /translation="MRIIFTIVIVFIFSVTSVISQSLSGQASESYKGALADSSRMANL
                     SWAEMMSDQSINFYAVRDAFNQFWAGRTPGKGKGYKPFRRWEAMMAPRVYPTGQRLVT
                     DAEIMKRFKEFEQQYSAQRNSGKSLTGTWSALGSNGAPSGSPSNQPGGTGRVNCIAFN
                     PLNTNIMYIGAPSAGLWRSNNGGSSWNPVNDTFAVVGVSSVVINHIDTSVMYIATGDG
                     DAGDTYTVGIMKSTNSGQSWHYTSFHPSVQSGYLIRKLVMHPTNPDIMWAATNGGILK
                     TTDGWASYTVASGIGGTFDIELKPGDPSTLYASTYTAFYKSTDGGTTFTAVTLPTPPA
                     SGFLRIAIAVSAAAPNNVYVVCGRNDASPYTNYAGFGGFYTSTNSGGSFTETFNETSS
                     NHNLLGWSPTFSDQGGQSWYDLDITVNPGNANE"
     gene            1687..2322
                     /gene="recR"
                     /locus_tag="PKY63_10855"
     CDS             1687..2322
                     /gene="recR"
                     /locus_tag="PKY63_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPF01157.1"
                     /translation="MFFEQLPSKYLENAVSELSKLPGVGRKTALRLALHLLRQDKSQS
                     NALGNSLIELREKITYCSVCHNISDTETCPVCANPSRDRSLICVVEDIRDVMAVERTS
                     QFNGVYHVLGGLISPIDGIRPQDIFLDSLQNRVQQNPIKEIILALSATVEGDTTSFYI
                     YRQLQHMNITFSTISRGISIGDELEYIDEVTLGRSIQARVPYQQQLVSEKS"
     gene            2333..3595
                     /locus_tag="PKY63_10860"
     CDS             2333..3595
                     /locus_tag="PKY63_10860"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPF01158.1"
                     /translation="MKINSRYLLFIFMLSCIAITIGCVSYVKMSSENMAPAYEEDFQP
                     LCQSAYIVHQSDSLSELILKLDPDGFLYKREKPSEPFMADATIRIVLFDSYSDNRWKD
                     STGISIRFEKDSIQGLFQSKIPLQLNTGQNTIAYVAIRDNNKDSRQTLMLLIDKTGRF
                     GSQWFVPDEATTGIPSAFTINAGSDITFAYHGDSPDKMKCYYFNDSFPLPSPPYALEN
                     IVPQKLDPDSVFNLLFQTDIGGFCIKTKKTGLYFFSADPSMAEGFTVVCTAKGFPDIN
                     THAAMLKPIRYISTQKEFRNIAEANNTVAAIEGFWLKIGKQEERTRNLIRKYYSRVMN
                     ANRLFTSYTEGWQTDRGMIYIVFGPPNIAYRSDENESWIYGEENNFFSITFTFEKIKN
                     KFSSNDYSLQRAPVYKDNWFRAVDIWRQ"
     gene            3597..4343
                     /gene="rlmB"
                     /locus_tag="PKY63_10865"
     CDS             3597..4343
                     /gene="rlmB"
                     /locus_tag="PKY63_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPF01159.1"
                     /translation="MKETDFIFGIHPVREAIEKETPIDHILIATGSDRKPGISEILSD
                     ARQQHIQIKRVPVEKLMRVTRGNHQGVIAFIAPVHYYKLEDLVMNAFEKGITPLFLIL
                     DNITDVRNFGAMARSCDAAGVTGIVIPETKSVSVTADAVRASAGALLNVPVVKTKNMN
                     GALKYMQQSGIRLFAASEKAVKLYSEADFNVPAGIILGSEEDGISAPILKISDEWIRI
                     PMAGQVASLNVSVSAGIIVFEAVRQRGLVI"
     gene            4382..4858
                     /gene="coaD"
                     /locus_tag="PKY63_10870"
     CDS             4382..4858
                     /gene="coaD"
                     /locus_tag="PKY63_10870"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPF01160.1"
                     /translation="MKRIAVFPGSFDPITTGHECIVRRAALLFDELIVAVGVNADKKS
                     YFPVEKRIEWLKTTFMDLPNVHVDSYHGLTVEYCISKNAEYILRGLRTAADFEFERSV
                     GQVNKMLHADIENIFLLSRPEHTPVNSSIVRDIHRNGGNIQPFVPAIVAKQIMEWQ"
     gene            4849..6357
                     /locus_tag="PKY63_10875"
     CDS             4849..6357
                     /locus_tag="PKY63_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPF01161.1"
                     /translation="MAIRGRSLRYFPKDTLMSFRIIVVVLSLLYAVNLSAAKTIIKGY
                     APGAEKLCIRLYIYDDYISNRESLLANTVIDSSGHFQFSLNVYENQVISGFFRIMDFT
                     SSELYIPANKNYDIEFDAFDYKDPNRIHVPLLSTIQLQYHFRNIDSTDINRQISAFNN
                     EYSAFIIESSGIIRNTSSYSLKRPPKHKVDSFVNTVTAKYLQINNVFFKNYCNYSLAL
                     LQLNMQSKSRKYLFDTYIYNKPILYDNVAYMDFFSNFFSDFIFGTSRKIQPYDVVLNI
                     NLKPNLNGLTDSLGKDSMLRNEQLREAVMLLNMRDWYSSKTIKQDSLMKVLNLYAEKT
                     KFDIQARIARNLVFMLTHLNAGNKAPAMNFKSLEGNVFNNDSLKNKYTFILFFTTWSK
                     PCLSELLVTNDLYQTWKDSIRFVGVSMDREPLKLFYFLEDNKFNFPIYHFGGDWVMAE
                     NLGLAAYPHGMFIDKNGNLIDYCTPLPSRGLIELFQKNAVKKVISHMPDVGQ"
     gene            complement(6591..7202)
                     /locus_tag="PKY63_10880"
     CDS             complement(6591..7202)
                     /locus_tag="PKY63_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF01162.1"
                     /translation="MYKVFIKDKVINFVETGSFSAQPEEIFIDPSFRDIQTLLNDIDS
                     DEQHTDFYFASEDAEKLFISFYTRMRIITAAGGTVVNENNEVLFIFRRGRWDLPKGKI
                     DNEETEQQAALREVTEETGLQNIQLGQRLSSSYHVYTIGREWILKETHWFIMTGDVTE
                     PLMPQADEGITEIKWVAKNSLTKMSQLVYRSLRGVVQDVAGIV"
     assembly_gap    7317..7416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7441..8493)
                     /locus_tag="PKY63_10885"
     CDS             complement(7441..8493)
                     /locus_tag="PKY63_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF01163.1"
                     /translation="MNPIFSKLLPGLLPLIVYFIADEFFGTVIGIAVALGFGIIQLFY
                     IRFRQKKWDRFILFDTALLIALGAVSLLLEDDKLIYWKFVFMDLMLIGFILFSLFSKK
                     NLFLLMSKRYVGDIEMNDNQTRAFNTNLWAFLIIAVLHAGLTSFSILFLENSATLFIG
                     GTLLYILLGVWILFVFIHQRLKRIKVMEEFVPLVDEEGKIIGRAARSDVHNGSFLMHP
                     VVHLHVIREDGAFLLQLRPKDKQIQPGKWDTAVGGHVAWGEPIEKALQRETEEEIGLK
                     KFKPLFAGKYVWKSEVEKELVFVFVYRATIDDKFNHTAEVADLRWWTKQELKRNLKKG
                     VFTPNFEYDYSMLMKE"
     gene            8583..>9316
                     /locus_tag="PKY63_10890"
     CDS             8583..>9316
                     /locus_tag="PKY63_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPF01164.1"
                     /translation="MKNGKTIGLALSGGAAKGFAHLGVLQAMEETGYKPDFISGTSAG
                     AIAAAFYADGYKPHEILKILCSYKLSDYFKPGWAGAGLMKSSGFYRILYENLFTENIE
                     ALPIPIWICITNLNTGLAEYHNHGNLARKVLASSSIPIFFRPVVMNNQLMVDGGVCDN
                     LPIKPIRQKADFTIAVNVNPIAPKIIKPGIKNIAERVFHLSINSRLQDMSIQPDLLIE
                     PKGASGFGYFNLKHAEKHFEIGYESA"
BASE COUNT         2846 a         1988 c         1782 g         2600 t
ORIGIN      
        1 atttcattgg catttcccgg attgacagta atatcaaggt cataccacga ctggccaccc
       61 tgatcgctga aagtaggaga ccatcccaac agattgtggt ttgatgaagt ttcattgaaa
      121 gtttcagtaa aagatccgcc gctgttggtt gatgtataaa aaccgccaaa accagcataa
      181 ttggtgtaag gcgaagcatc gttacgacca cagacaacat aaacattgtt tggagcagca
      241 gccgacactg caatggcaat tcgaagaaat ccgcttgcag gaggcgttgg caatgtcaca
      301 gcagtgaaag ttgttccgcc atcagtcgat ttgtaaaatg cagtatatgt cgaagcatac
      361 aaagtgctgg gatcacctgg tttgagttca atgtcaaaag ttcccccaat acccgaagca
      421 actgtatatg aagcccagcc atcggtggtt ttcaatattc caccattagt ggctgcccac
      481 attatatccg gatttgtcgg atgcattaca agcttacgga tcaggtatcc gctttgtacc
      541 gagggatgaa aagaagtgta atgccagctc tgaccgctat tggtcgattt cataatcccc
      601 acagtataag tatcaccagc atcaccgtca ccagtagcaa tatacataac agacgtatca
      661 atgtgattga tgactacact cgacactcca accacagcaa aagtgtcatt caccggattc
      721 caactcgagc cgccattatt actgcgccat aaacctgccg aaggagcacc gatatacatt
      781 atattggtat tcaatggatt aaacgcgatg cagttaacac gacccgtccc gccgggctga
      841 ttactcggag atccggaagg agcaccgttg gaacccaatg cagaccatgt accggtcaaa
      901 cttttacctg aatttctttg agcagaatac tgctgctcaa attccttgaa acgcttcatt
      961 atttcagcat cagtcaccaa acgctgtccg gtaggataaa ccctcggggc catcatagct
     1021 tcccaccgtc gaaacggttt ataacccttg ccttttcctg gagtgcggcc agcccagaat
     1081 tgattgaaag catctcggac tgcataaaaa ttaatgcttt gatccgacat catttcagcc
     1141 cagcttaaat tggccattcg ggaagaatca gcaagcgccc ccttgtaaga ttcggatgcc
     1201 tgacctgaca gtgattgaga aattacactg gtaactgaaa atataaaaac aatgacaatg
     1261 gtgaaaataa ttctcatatg gggggaaatt agtgttattt gacttcgttg aaatgcaaaa
     1321 ataaaacgaa ctacaagaat aatgaaaaaa agaaacaaaa aagccgtaaa tataaccaac
     1381 acaacctgag tgtaaaacca ataaaatgac tgatgcagat gaaaaaaaag tggagttttt
     1441 ctgaggaaaa ttttaaaata caacattgat tttcaaatac ttaaaatttt attttttata
     1501 caaagatgtg atttgcaaaa tcacatcttt gtataaaatt cccaacctat aaatggcata
     1561 caatactcct cttttcccca aaaactcaaa cccatcaaaa aacattttca ttcataccca
     1621 gttcattgca aaaccagtat tgttcatttc gctgcatttt tgtactttta caaaaaaaaa
     1681 taccgcatgt ttttcgagca attgccttca aaataccttg aaaatgcagt gtcagaatta
     1741 tcaaaattgc cgggcgttgg tcgcaaaaca gcgttgcgat tggctttaca cttactgaga
     1801 caagataaat cgcagtcaaa tgccttgggc aacagtctga tcgaattgcg tgaaaagatc
     1861 acctattgtt ctgtttgtca taacatcagc gacactgaaa cctgtccggt atgtgccaat
     1921 ccgtcgcgcg accgatcgct tatctgcgtg gttgaagaca ttcgcgacgt catggctgtc
     1981 gaaagaactt cgcaatttaa cggcgtttat catgtacttg gcggattaat atcaccaatc
     2041 gacggaatcc ggccgcagga tatttttctt gattctttac agaatcgggt tcaacagaat
     2101 cccatcaagg aaatcattct ggctttatcg gccacagtag aaggcgacac gacctctttt
     2161 tacatttatc gtcagttgca gcacatgaac atcaccttca gcactatttc aagaggtata
     2221 tccattggag atgaacttga atacattgat gaagtaacgc ttgggcgatc gattcaggca
     2281 cgtgttcctt atcagcagca acttgtttct gaaaaaagct gaaattgttt taatgaaaat
     2341 caacagccgg tatttattgt tcatttttat gctttcctgt atcgcaataa cgataggatg
     2401 cgtatcatat gtgaaaatgt cgagcgaaaa catggcgcct gcttatgagg aagattttca
     2461 gccactgtgt caatccgcct atattgttca tcagagcgac agtttatcag aattgatttt
     2521 aaaacttgat ccggacggat ttttatacaa gcgcgaaaaa ccatcggagc catttatggc
     2581 agatgcaaca atacgcattg tcctttttga ttcctacagc gataatcgat ggaaagacag
     2641 taccggaatc agcattcgtt ttgaaaagga cagcattcaa gggctttttc aaagtaaaat
     2701 tccgttgcaa ctcaatacgg gtcaaaatac cattgcatac gttgctattc gcgacaacaa
     2761 caaagacagc cggcaaaccc tgatgttatt gattgacaaa acaggccgat ttggttctca
     2821 gtggtttgta cctgacgagg ccacaacagg tattccatct gcatttacaa taaatgccgg
     2881 ttcagatatt acctttgctt accatgggga ttcgccggat aaaatgaaat gttattattt
     2941 caatgattct ttccctcttc cttcaccgcc atatgcactt gaaaacattg tgcctcaaaa
     3001 acttgacccg gactcagttt tcaatctctt atttcagact gacattggtg ggttttgcat
     3061 taaaacaaaa aaaacgggac tatatttctt ttcggccgat ccatcgatgg ctgaaggatt
     3121 tactgttgta tgcactgcca aaggatttcc ggatatcaat acacatgcag ccatgctgaa
     3181 gcccatccga tacatttcaa cacagaaaga attcagaaac attgccgaag caaataatac
     3241 cgtagcagcc attgaagggt tctggcttaa aataggaaag caggaagagc ggacccgcaa
     3301 tcttatccga aaatattatt cacgggtaat gaatgccaac cggcttttta ccagctacac
     3361 cgaaggatgg caaaccgacc gcggaatgat ttacatcgtt tttggtccgc ccaatattgc
     3421 ttatcgttct gatgaaaatg aatcatggat ttatggagaa gaaaataatt ttttttccat
     3481 cactttcact tttgaaaaaa taaaaaacaa attcagtagt aacgactaca gtctgcaaag
     3541 ggcaccggtt tacaaagaca actggtttcg tgcagttgat atctggagac aataatatga
     3601 aagaaacaga ttttattttc ggcattcatc ccgtacggga ggccattgaa aaagaaacgc
     3661 ccatcgatca cattctgata gcaacaggat cggatcgcaa accgggcatt tctgaaatac
     3721 taagcgatgc acgtcagcaa cacattcaga taaagcgtgt gcctgttgaa aagcttatgc
     3781 gtgttacccg tggcaatcat cagggcgtca ttgcttttat tgctccggtg cattactaca
     3841 aactcgaaga ccttgtaatg aatgcttttg aaaaaggcat cactccattg tttctgatat
     3901 tggataatat caccgatgtc cgtaattttg gtgccatggc acgcagttgc gatgcagcag
     3961 gcgttacagg aattgtcatt cccgaaacaa aaagcgtttc cgttactgcc gatgccgttc
     4021 gggcatcagc cggagctttg ctcaatgtgc ctgttgtaaa aacaaaaaac atgaatggcg
     4081 ctttaaaata tatgcagcaa agcggaatac gcttatttgc ggccagcgaa aaagccgtga
     4141 aactatacag cgaagctgat ttcaatgtgc ccgccggcat tattcttggc agcgaagaag
     4201 atggaatttc agctccgatt cttaaaattt ccgatgaatg gatccgtatt cccatggccg
     4261 gacaggtggc atcgctcaat gtttcggtca gcgccggaat tattgttttc gaagcggttc
     4321 gtcaacgtgg tcttgtaatt tgatctattg ttttttatat ttgaattaaa ttttttctat
     4381 tatgaaaaga attgctgttt ttcccggatc cttcgacccg ataaccacag ggcacgaatg
     4441 tatcgttcgc cgtgctgcac tactcttcga tgaactcatc gtggccgtag gcgtcaatgc
     4501 cgataaaaaa tcctatttcc cggtggaaaa aagaatcgaa tggttgaaaa caacctttat
     4561 ggatctgccc aatgtgcatg ttgattctta tcacggactc actgttgaat actgcatcag
     4621 caaaaatgct gaatacattc tacgcggttt gcgcaccgcc gccgatttcg aattcgaacg
     4681 cagcgtaggt caggtaaaca aaatgctgca tgccgacata gaaaatatat tcctgctttc
     4741 ccgccccgaa catacaccgg tcaattcatc cattgtgcgc gatattcatc gcaacggtgg
     4801 caatattcaa ccttttgttc ctgccattgt tgcaaaacaa ataatggaat ggcaataaga
     4861 ggccgatcgc tgcgttattt tccgaaagac acgcttatga gtttcagaat cattgttgtt
     4921 gttttgtcat tactctatgc tgtcaatctg tctgcagcaa aaacaatcat taaaggctat
     4981 gctccgggtg cagaaaaact ctgtattcgc ctgtatatct acgatgatta tatcagcaat
     5041 cgggaatctt tgctggccaa tacagttata gacagctccg gccattttca gttttcgctc
     5101 aatgtctatg aaaaccaggt gatcagcgga ttctttagaa taatggattt tacaagcagc
     5161 gaattgtaca ttccagccaa taaaaattat gatattgaat ttgatgcttt tgattacaaa
     5221 gacccaaatc gcattcatgt accattactt tctacaatcc agttgcagta tcatttccgc
     5281 aatattgatt ccacagatat caaccgccag atttctgcat tcaataatga atattctgct
     5341 tttataattg aaagttccgg aatcatcaga aacacttctt cttattcact aaaacgacca
     5401 ccaaaacaca aagttgatag ttttgtaaac accgttactg ctaaatacct tcaaatcaat
     5461 aatgtttttt ttaaaaatta ctgcaattat tcattggcct tgctgcaatt gaatatgcaa
     5521 tcgaaaagca gaaaatattt attcgacaca tatatatata ataaacccat tttatatgat
     5581 aatgtggcct atatggactt tttcagcaat ttcttttctg attttatttt tggaacatcc
     5641 agaaaaatac aaccctacga tgttgtctta aacatcaatc tgaagcccaa tctaaacggg
     5701 ttgacagatt cgctgggaaa agactcaatg ctgcgcaacg aacaactacg cgaagctgtc
     5761 atgctgctca atatgcgcga ttggtattca tcgaaaacca tcaaacagga tagtctgatg
     5821 aaggttctga acctgtatgc agagaaaaca aaatttgata ttcaggcgcg catcgcacga
     5881 aaccttgttt tcatgcttac acatctcaat gctggaaata aagctccagc aatgaatttc
     5941 aaatcacttg agggcaatgt tttcaataac gattctttaa aaaataaata tacttttata
     6001 ttgtttttta caacctggag caagccatgt ctttctgaat tgttggtaac caatgatctt
     6061 tatcaaacgt ggaaagacag tatccgtttt gttggtgtaa gcatggaccg tgagccattg
     6121 aaattatttt attttctgga agacaacaaa ttcaatttcc ccatatatca ttttggcgga
     6181 gactgggtaa tggccgaaaa ccttgggctg gctgcatatc cgcatggaat gttcattgac
     6241 aaaaacggaa atctgattga ttattgcact cctttaccca gtcgtggttt gatcgaattg
     6301 tttcagaaaa atgccgtaaa aaaagtcatc agtcatatgc cggatgttgg gcaataaggt
     6361 caaagtcaag atcgaggaca aggtcaaggt ttcagtggtg agcagcgagc ggtgatcggt
     6421 gatcggtgaa ttttgctgat tattcaatgt atcgctcttc tttccttttt cagctattgc
     6481 tccgaccgta aacgccagag agatagaaaa atctaaatcg ttgaattttt tatcagcaaa
     6541 agaaggtcac tgctcactat tcaccgctca ctgggatgaa ggaatctcat ttatacaatc
     6601 ccggcaacat cctgcaccac tccgcgcagt gagcgataaa caagttgcga catttttgtc
     6661 agactatttt ttgcgaccca tttaatttca gtaattcctt catcggcttg cggcatcaac
     6721 ggttcagtca catcgcctgt cataataaac cagtgtgttt ctttcaatat ccattccctc
     6781 ccaatggtgt aaacatggta tgatgaagag agtcgttgtc ctaattgaat attttgcaat
     6841 ccggtttctt cagtaacttc acgcaaggca gcctgttgtt ctgtttcttc attgtcgatt
     6901 tttccttttg gcaaatccca gcgcccgcga cggaaaataa aaagcacttc gttgttttcg
     6961 ttgacaacag tgccaccggc tgcggttatt atgcgcatgc gtgtgtaaaa ggaaatgaaa
     7021 agtttttccg catcttccga agcaaaataa aaatccgtgt gttgttcatc actgtcaatg
     7081 tcattgagca aagtctggat atcccggaac gaaggatcaa taaaaatttc ttcaggctga
     7141 gccgaaaagc tgcctgtttc aacgaaatta ataactttgt ccttgataaa aactttatac
     7201 attcgtgcta tgatttacac acaggaaaca gccagagaaa ctgcccgttt gcttctgcaa
     7261 ataaacgcaa ttaaattgaa tcccgcaaat ccgtttacct gggcaagcgg atggaannnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgga gagacattac aatacataac
     7441 ctattctttc atcagcatcg aatagtcata ttcaaaattc ggcgtaaaga cacctttctt
     7501 taaattacgc tttagttcct gctttgtcca ccaacgcaaa tcggccactt cggcggtatg
     7561 attgaatttg tcatctatcg tagcccgata gacgaaaaca aaaaccaact ccttctctac
     7621 ttcgcttttc cagacatatt ttccggcaaa caaaggtttg aatttcttca atccgatttc
     7681 ttcttccgtc tcacgctgca gcgctttttc aatgggctca ccccatgcaa catgccctcc
     7741 aacagcagta tcccatttac ccggctgaat ctgcttatcc ttaggacgca actgaagcag
     7801 aaatgcacca tcttcacgaa taacatgcaa atgcactacc ggatgcatta aaaaagaacc
     7861 attatgaaca tcgctgcggg ctgctcgtcc gatgattttt ccttcttcat caaccaacgg
     7921 aacaaattct tccataacct ttatacgttt caaacgttga tgaataaata caaataaaat
     7981 ccacacacca agcaatatat aaagcaatgt tccgccaata aataatgttg ctgaattttc
     8041 aagaaacaat attgagaacg acgttaatcc ggcatgcaaa acagcaataa tgaggaaagc
     8101 ccacagattc gtgttgaagg ccctcgtctg gttgtcgttc atttcaatgt caccgacata
     8161 tcgtttcgac atgagcagaa agagattctt ttttgagaac agcgaaaaca gaatgaatcc
     8221 aatgagcatc agatccatga atacaaactt ccaataaatc agtttatcat cctccagaag
     8281 caatgatact gcgccaagtg caatgagtaa ggccgtgtcg aataaaatga aacgatccca
     8341 ttttttctgc cggaagcgga tataaaaaag ctgtatgatg ccaaacccca aagcaactgc
     8401 aatgcctatg actgtcccga aaaattcatc agcaataaaa tatacaatga gtgggagaag
     8461 cccggggagc agttttgaaa atatcggatt catttctgga aattgaaatt tcaactgtaa
     8521 aaatacatat ttaaaaggaa ttgctacatt tgatttttcg attcagaaaa catcttacgg
     8581 atatgaaaaa cggaaaaaca attggtcttg cattaagtgg aggagcagcc aagggctttg
     8641 cccatctggg agtgttacag gccatggaag aaaccggata taaaccggat ttcatatcag
     8701 gtaccagcgc cggcgccata gcagccgcat tttatgctga tggatacaaa ccacacgaaa
     8761 tactgaaaat tctgtgctcc tacaaattgt ccgattactt caaacccggc tgggctggcg
     8821 caggactcat gaagtcaagc ggtttctacc gcattttgta tgaaaatctt tttactgaaa
     8881 acattgaggc attacctatt ccaatttgga tttgcattac caatctgaat actgggcttg
     8941 ccgaatatca caaccatgga aaccttgcac gtaaagtgct ggcatcttct tccattccca
     9001 tttttttcag gccagttgta atgaacaatc aactgatggt cgatggtggt gtgtgtgaca
     9061 atctgccaat aaaaccaatc aggcaaaaag ctgattttac aattgcggta aacgtaaatc
     9121 ctatcgctcc aaaaataatc aaaccaggaa tcaaaaacat tgctgaacgg gtatttcatt
     9181 tgtcgataaa cagccgtctc caggacatgt cgattcaacc cgatctgttg attgaaccca
     9241 aaggagcatc tggctttgga tattttaatc tgaaacatgc tgaaaaacat tttgaaatcg
     9301 ggtatgaaag tgcttt
//
LOCUS       DAOPKE010000104         9259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_7861_length_9259_cov_11.006193, whole genome shotgun sequence.
ACCESSION   DAOPKE010000104 DAOPKE010000000
VERSION     DAOPKE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7861_length_9259_cov_11.006193"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1236)
                     /locus_tag="PKY63_10895"
     CDS             complement(106..1236)
                     /locus_tag="PKY63_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01165.1"
                     /translation="MATLKVLIVDDEPGIRSGISRILKNFSVDFPFIDEAFTFESIEA
                     GTGEEALDILNAGPIDIVLLDNKLPGIHGIEVLEHINQKQIDTTVMMITSYASLDLAI
                     KATNNGAYNFVPKPFTPQELKTAMEGITKHVYLSRMTKQLNTEGKQIRFQFLSVLSHE
                     LKSPINAVEGYLRIMQDKQVGSNLEDYETMIDRSLERIRGMRSLIMDMLDLTKLENGK
                     KKREITKVDLVQIARLSIDTMTPMAIQKNVKVFIDSDEPSVYLNADSSEIEIIFNNLV
                     SNAVKYNVQDGSVTILIKSEGNKIKVSVADTGIGISEEDITKLFQDFSRIKNERTKGI
                     SGSGLGLSIMKKMVEEYYHGQVSVISEPDKGSTFIVEIPQNS"
     gene            complement(1249..1644)
                     /locus_tag="PKY63_10900"
     CDS             complement(1249..1644)
                     /locus_tag="PKY63_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF01166.1"
                     /translation="METKKKTVLIVDDDIDSMTQLTMHMTKMGFNVISADNQKEGEEL
                     INANKPDLAIFDLMMDNKDSGFILSYKIKKKYPDVPVIIATAVTAETGMLFGLNTEEE
                     KKWIKADLYLEKGLRYDQLEIEVGKLLNL"
     gene            complement(1658..3646)
                     /locus_tag="PKY63_10905"
     CDS             complement(1658..3646)
                     /locus_tag="PKY63_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPF01167.1"
                     /translation="MGNLINNELVYTVKDRCRVCYTCVRECPVKAIKIINGQAEVISS
                     RCIGCGNCTKVCSQGAKVFVDTTQGVYSLLSSNNKVAALVAPSFAAEFSDIADYRLLV
                     GMLRQMGFEYVVEVGFGADLVGKEYKKVMDDHEAEKTISSDCPAIVYYVRHYFPELTP
                     YLAKIVSPMVAMDRIMKKKHGDDLKTVFIGPCIAKKAESAEVDHVITFKELRDMFEKL
                     NLNPQTTTSSDFDPPKAGRAAIFPVTRGKLHSINKSDNICDENIIIASGRVHFREAIK
                     EFAEDLNRSQHLELLCCEGCIMGPGMTNNNRIFTRTMNLRNYVRDKLTNFDKEAWKRE
                     YEAAKDIDYSQHFEPADRRIPTPSDEEIQKALMAMGKMTPADHLNCGACGYDTCVEHA
                     IAIVEGLAETEMCLPFTIEKLHDSINNLNLSNEMLASAQQSLKQSEKLANMGQLSAGI
                     AHELNNPLGIITMYSSILMDEAPTESPVRKDLELIVEQADRCKKIVGGLLNFARKNQV
                     NLVETNVVTFTHHSIDSIILPTNIKIDFQSNLTNPFAWIDNDQWMQVLTNIEKNAIEA
                     MPDGGTLKIEINEAGDEIEFSIGDTGVGISKENLDKIFTPFFTTKPIGKGTGLGLPLI
                     YGIVKMHNGQINIETNDDPSQGQTGTCFKIKIPRQKKL"
     gene            complement(3686..5410)
                     /locus_tag="PKY63_10910"
     CDS             complement(3686..5410)
                     /locus_tag="PKY63_10910"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPF01168.1"
                     /translation="MSLTIEINGNKYEAAQGETILTVLNRNGIKVPTLCHMKDFVPTG
                     ACRMCVVEMEGNGKLVSSCSFPVADGMKIRTHSPRVVEARKSIVELLLSNHPDDCLYC
                     IRNGNCELQSLSKEYHVVDRRIRGVKNNHKLDHSSLSITRDPDKCILCGRCVRVCEET
                     MGVSCIEFINRGSKTVIGATMNKGLNTSSCVNCGRCVMVCPTGALSERNQIAEVQSML
                     HQTEKTVIVQYAPSISVSLAEEFGMKPGQDINGILNAALRKMGFKFVFDTTFGADLTI
                     MEESAELIDRIVKGKTLPMFTSCCPAWIKFAEEFAPEYLENLSSCKSPQQMTGAIIKN
                     YFAEQVNLKPQDILSVSIMPCTAKKFEAQRDTMGREGYNDVDLVLTTRELIELIHLYG
                     IDMHQIEPQLTDSPLGVRSTAGKIFGASGGVMEAALRTAYKKLTGNELTNFKINAVRG
                     MKGRKEAKIKINDLELGVAVVSGLANARTLIDEIRNGRNDLHFIEVMTCPGGCIAGGG
                     QRIGIDESVIEARMQSLYEIDDKESLKVSHKNPEIIELYDNFLGEPLGHKSHDLLHTH
                     YIKREVLL"
     gene            complement(5425..7344)
                     /locus_tag="PKY63_10915"
     CDS             complement(5425..7344)
                     /locus_tag="PKY63_10915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPF01169.1"
                     /translation="MNNSIKKYLENLAASNAKGISVALPEKETDSKKTIVFIGAGTCG
                     LGAGAAKTKAAIEEYLKNNNIDGEIIEVGCIGFCAVEPIMDIKLPGKNRISFSKVTAD
                     KVNGVFEQVMSGNIDQQNVLGQFITADLKPWDNIRNIQEHPFFVKQKRLVLKNCGVID
                     PVSMDEYIMNGGYAGLIKALKTMSGEDVIDTIEKSGLRGRGGGGFPTGTKWRLAHKTP
                     ADQKYMICNADEGDPGAFMDRAVIEGDPHRLLEGLAIGAYAIGATVAYIYIRAEYPLA
                     IERLKIAIKQAEDYGLLGHNIMGSGFDLKIKIKMGAGAFVCGEETALMHSIEGYRGMP
                     SPRPPFPAESGLFGKPSCINNVETFANVSEIILKGADWFSSTGTEKSKGTKVFALSGN
                     VKNTGLVEIPMGTTVREIIFDIAGGIAFDKKFKAVQIGGPSGGCITEENLDIEIDYQN
                     LIAVGAMMGSGGLVVMDENTCMVDIAKFFMDFIQRESCGKCIPCREGTKRMLEILQSI
                     TSKPAPGDNNAALERFQGVLQLEKLAKVIKDTSLCGLGQSAPNPVLSTLRYFRDEYEA
                     HIFDRVCPAGKCTDLRKFRIIVDKCTGCTACTKKCPTGAIIGTKKNPHFIQEDKCIGC
                     GACYETCKFEAIEMY"
     gene            complement(7350..7811)
                     /gene="nuoE"
                     /locus_tag="PKY63_10920"
     CDS             complement(7350..7811)
                     /gene="nuoE"
                     /locus_tag="PKY63_10920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPF01170.1"
                     /translation="METTIDKILANYENCGRDSLIPLLQEIQEKEGYLSEEAIVKTGN
                     KLKVPTSKIYGVATFYNQFRFEPVGRYHIQVCRGTACHVLGSATVLQELESILKIKAG
                     QTTRDGLFSIEVVACIGACGLAPVICINGEFFAKVTTEKIKEIIETYRAKE"
     gene            complement(7847..8341)
                     /locus_tag="PKY63_10925"
     CDS             complement(7847..8341)
                     /locus_tag="PKY63_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF01171.1"
                     /translation="METKKILIVDDDIDVITIIETILKKEGYTVISANDKKEGMKKIR
                     EEKPDLAILDVMMTTHYEGFELAKEITDDAELSKMPVLMQTSIDILTTTKPDVQAMAR
                     EFRKNPGFKDLHVILVKDINSGKAGVDYLSEDGRSIWFPVDGFVRKPVDAKKVVPEIK
                     RILG"
     gene            complement(8364..8756)
                     /locus_tag="PKY63_10930"
     CDS             complement(8364..8756)
                     /locus_tag="PKY63_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF01172.1"
                     /translation="MTRVIIVDNEPTFRKGLKAILTNIGNVEVVGEASNGEEFLKLID
                     ETSADIVFMDVKMPVLDGVEATRKARMSHPEMHIIGFSSYENEDYVNRMVEAGACGYL
                     TKSGDNYDLLSQIINNPEAGIFPGAKKF"
BASE COUNT         2452 a         2121 c         1838 g         2848 t
ORIGIN      
        1 agatatccct gaatttcagc tgcatcaata aatggctgca tcggaacatc gggcatgttg
       61 tatttttccg gagtgaattt cattgtgttt ctcctttttt atacctcagg aattctgagg
      121 aatttctacg ataaatgtac ttcctttatc aggttcgcta ataacactaa cctgaccatg
      181 ataatattct tcaaccattt ttttcatgat tgacaaccca agtccgctgc cactgatccc
      241 tttggttctc tcatttttaa tacggctgaa atcctgaaag agctttgtta tgtcttcttc
      301 acttattcca atgcctgtat cagccacaga aactttaatt ttgtttcctt ccgattttat
      361 cagaatagtg acagaaccat cctgcacatt gtattttact gcatttgaaa ccagattgtt
      421 gaaaatgatt tcaatttcag aactgtcagc gtttaaatat acactgggtt catcgctgtc
      481 gataaaaact ttaacgtttt tctgaatagc cattggggtc atggtgtcga ttgacagacg
      541 tgcaatctgt accagatcga ctttagtaat ctcacgtttt ttctttccat tttccagttt
      601 ggtcaggtcg agcatatcca tgatgagcga acgcattcct ctgattcgct ccagcgaacg
      661 gtcaatcatg gtttcataat cttccagatt gctgcctacc tgtttgtcct gcataatacg
      721 aagataacct tccactgcat tgattggaga cttcagctcg tgagataaca ccgacaggaa
      781 ctgaaaacgg atttgtttgc cttcggtatt gagctgcttg gtcatacgcg aaagataaac
      841 atgcttggtt ataccttcca tagccgtttt cagctcctgt ggtgtgaagg gtttgggtac
      901 aaaattgtat gcgccattgt tggtggcttt tatcgcaagg tcgagcgatg catacgaagt
      961 tatcatcata accgttgtgt cgatttgctt ctgattaata tgctccagga cttcaattcc
     1021 atgaattccc ggcagtttat tatccagaag aacaatgtcg atgggtccgg cattgagaat
     1081 atccaaagct tcttcgccgg ttcctgcttc aatcgactca aatgtgaacg cttcgtcaat
     1141 gaaagggaag tccaccgaga aatttttcag aatcctcgaa attccggagc ggatgcccgg
     1201 ttcatcgtcc acaatcaata ctttaagcgt tgccatggta tattgttttt aaaggtttaa
     1261 gagcttacca acttcaattt cgagctgatc gtagcgtaat cccttttcaa gatacagatc
     1321 ggctttgatc catttctttt cttcttcggt attaagtccg aacaacattc ctgtttcagc
     1381 ggtaactgct gttgcaatga taacaggaac atcgggatat ttctttttaa ttttatagct
     1441 gagaataaaa ccactgtcct tattgtccat catgaggtca aaaatggcca ggtcaggttt
     1501 gttggcatta atcagctctt cgccttcttt ctgattgtcg gccgaaatga cattgaatcc
     1561 catttttgtc atgtgcatgg tcagctgggt catgctgtca atatcgtcat ccacaatcaa
     1621 cactgttttc ttcttggttt ccataactat ctttttttta caattttttc tgtcttggaa
     1681 ttttgatttt aaaacacgtt cctgtttgtc cctgtgaagg atcatcattg gtctcgatgt
     1741 tgatttgtcc gttgtgcatt ttcacaattc cgtaaatcaa aggcaatccg agacctgtac
     1801 ccttgcctat cggttttgtt gtgaaaaacg gggtgaaaat tttatcaagg ttttccttcg
     1861 aaatgccaac acctgtatca ccaatactga actcaatttc atcaccggct tcgttgatct
     1921 caattttcaa cgtaccacca tccggcatgg cttcaatggc attcttctca atgttggtga
     1981 gcacctgcat ccactgatca ttgtcaatcc atgcaaaagg gttcgtgaga ttcgactgaa
     2041 aatctatctt gatatttgtg ggcaaaatga ttgaatcaat gctgtgatgc gtgaatgtca
     2101 ccacatttgt ttcaacaaga ttgacctgat tttttcttgc gaaattcagc aacccaccta
     2161 caattttttt acaacgatcg gcctgttcaa caatgagctc caggtccttg cggacagggc
     2221 tttcagttgg agcttcatcc atcagaatgc tggaatacat agtgataatt cctagcggat
     2281 tattcagttc gtgtgcaatg ccagccgaaa gctgacccat attggcaagc ttttctgatt
     2341 gtttcagcga ctgctgagcc gaagcaagca tttcgtttga caaattcaaa ttattaattg
     2401 agtcgtgcag tttttcgatg gtaaaaggca aacacatctc agtttcggcc aaaccttcca
     2461 caatggcaat ggcatgttca acacaagtgt catagccaca agcaccacag ttaagatgat
     2521 cggcgggagt cattttcccc atggccatca aagctttctg aatttcttca tccgaagggg
     2581 tcggaatacg ccggtctgcc ggttcaaaat gctgcgaata atcaatatcc tttgccgcct
     2641 cgtattcgcg tttccatgct tccttatcaa aattagtgag tttatctctc acataattgc
     2701 gcaaattcat tgtgcgcgta aaaatccggt tgttattcgt cattccgggt cccataatac
     2761 aaccttcgca acaaagaagt tcgagatgct gcgaacggtt cagatcttcc gcaaattcct
     2821 tgatagcttc gcggaaatga acacgtcccg aagcaataat gatattctca tcacagatat
     2881 tatcggactt attgatggaa tgaagttttc cgcgtgttac cgggaaaatg gctgccctac
     2941 ccgctttggg cggatcgaag tctgaagacg ttgtggtttg cggattcaga ttgagttttt
     3001 caaacatatc gcgcaattcc ttaaacgtaa taacatgatc cacctcggct gattccgctt
     3061 ttttcgcaat gcagggccca ataaagactg ttttcagatc atcgccatgt ttctttttca
     3121 tgatgcgatc catggctacc atgggcgata caatctttgc gagatagggt gtgagctcag
     3181 gaaaataatg acggacataa tacacaatgg caggacaatc ggacgaaatg gttttttcgg
     3241 cctcgtggtc atccattact tttttgtatt ccttccccac cagatcggct ccaaatccca
     3301 cttcgaccac atactcaaaa cccatctgac gcaacatacc aaccaataaa cgataatcgg
     3361 caatatcgct aaactctgct gcaaagctgg gggcaacaag agcagcaacc ttattattgc
     3421 tgctcagcaa tgaataaaca ccctgggttg tatcaacaaa aacctttgca ccctgactgc
     3481 agacttttgt gcaatttcca catccaatgc aacgcgaaga aattacctcg gcctgaccat
     3541 tgatgatctt aatcgccttg accgggcatt ctctgacaca ggtataacaa accctgcacc
     3601 ggtctttgac cgtgtacaca agctcgttgt taatcagatt gcccattttc tttcctttta
     3661 tgatattagc tgaactgtcc accgattata ataacacctc gcgtttgata tagtgtgtat
     3721 gtaacaaatc atgactcttg tgacccagcg gttcgccaag gaaattatca tagagttcaa
     3781 tgatttcggg atttttatgc gacactttca acgattcctt gtcatcaatt tcgtagagtg
     3841 attgcatacg cgcctctatc accgattcat caatacctat gcgctgtccg ccaccggcaa
     3901 tacaaccacc gggacaagtc attacttcga taaaatgaag atcgtttcgt ccatttctaa
     3961 tttcatcaat cagggttctg gcattggcaa gaccgctaac aacagccacc cccagttcca
     4021 gatcattaat ctttattttt gcttctttgc gacctttcat gccacgaact gcattaattt
     4081 taaaattggt cagctcatta ccagtcaact ttttatatgc tgtacgcaaa gctgcttcca
     4141 tcacaccacc cgatgcgccg aagattttac cagccgtact gcgcactcca agcggagaat
     4201 cagtaagttg aggttcaatc tgatgcatgt caataccata gagatgaata agctcaatca
     4261 attcacgggt agtaagcacc aaatcaacat cattataccc ttcgcgtccc atggtatcgc
     4321 gctgtgcctc aaattttttg gcagtgcaag gcataatcga aacagaaaga atatcctgtg
     4381 gtttgaggtt gacctgttct gcaaaataat ttttaataat tgcaccagtc atttgctgcg
     4441 gtgatttgca acttgaaaga ttttcgagat attcaggtgc aaattcttcg gcaaacttta
     4501 tccatgccgg gcagcaactg gtaaacatgg gcagtgtttt gccttttaca atgcgatcga
     4561 tcaattcggc cgattcttcc ataatagtaa ggtcggcgcc aaatgttgtg tcaaacacaa
     4621 atttaaagcc cattttacgc aatgcagcat tcaaaatacc attgatatcc tgtccgggtt
     4681 tcatgccgaa ttcttcggcc agcgaaaccg aaatagaagg agcatattga acgattaccg
     4741 ttttttctgt ctgatgcagc atggactgaa cctccgcaat ctgattccgt tcagacaatg
     4801 caccagtagg acaaaccatc acgcaacgtc cgcaattgac acagctggaa gtgttgagtc
     4861 ctttgttcat tgtggcacca atcacggttt tacttccgcg gttaatgaat tcgatgcagg
     4921 aaacacccat ggtttcttca caaacacgta cacaacgacc acaaagaata catttgtcag
     4981 gatcgcgggt aatactcaga ctggaatgat ccagtttatg attgtttttc actccacgga
     5041 tacggcggtc aaccacatga tattcttttg aaagactctg caactcacaa tttccgttgc
     5101 ggatacaata caaacagtcg tccggatggt ttgaaagcaa cagctcaaca atggatttgc
     5161 gtgcttcaac tacgcgtgga gaatgggtgc ggattttcat tccgtcggca acaggaaagg
     5221 agcatgaact taccagtttt ccgttacctt ccatttcaac cacacacatg cggcatgcac
     5281 cggttggaac aaaatctttc atgtgacaca gcgttggtac tttaatacca ttgcggttga
     5341 gaactgtcag aatggtttcg ccctgagcag cttcgtattt atttccgttt atttcgattg
     5401 tcagactcat agttcaatgg ttttttagta catttcaatt gcttcgaatt tgcaggtttc
     5461 atagcaggca ccgcatccga tacatttgtc ttcctgaata aaatgcggat tctttttcgt
     5521 tccgataatg gcgccggtgg ggcatttttt tgtacaagcc gtgcaaccgg tacatttatc
     5581 cacaataatt ctgaatttac gcaaatccgt acatttccca gcaggacaaa cacggtcgaa
     5641 aatatgtgct tcgtactcat cacggaaata acggagtgtg cttaagaccg gatttggagc
     5701 cgattgacca agaccacaca atgaggtgtc cttgataact tttgccagtt tttcgagttg
     5761 caacacaccc tgaaaacgct caagggctgc attgttgtca ccgggagcag gtttagaagt
     5821 aatgctttgc agaatctcga gcatgcgctt ggttccttcg cggcatggga tacattttcc
     5881 gcagctttcg cgctgaatga aatccatgaa gaatttggca atgtcaacca tacatgtgtt
     5941 ttcatccatc accaccagac cgcccgatcc catcatggct ccaacagcaa tcagattctg
     6001 ataatcaatc tctatatcaa gattttcttc agtgatacaa ccacctgaag gaccaccaat
     6061 ctgcacagct ttgaattttt tatcaaaggc aattccaccc gcaatgtcga aaataatttc
     6121 gcgaaccgtt gttcccatcg gaatttcgac aagtccagta tttttcacat tacccgaaag
     6181 ggcaaatact tttgtaccct tgctcttttc ggtaccggta gaactgaacc aatcggcgcc
     6241 tttgagaata atttcggaaa cattggcaaa agtttcgaca ttattgatac aactgggttt
     6301 cccaaacaaa ccactttcgg ccggaaacgg cggacgaggg ctgggcatac cgcggtaacc
     6361 ttcgatactg tgcatcagag cggtttcttc accgcaaaca aatgcaccgg cacccatttt
     6421 aatttttatt ttgaggtcga atcccgaacc catgatattg tgtccgagca gaccataatc
     6481 ttcagcttgt ttaatggcaa ttttcaaacg ttcaatggcc agcgggtact cggcgcggat
     6541 ataaatatat gcaaccgttg caccaatagc ataagcgcca atagccagcc cttcgagcaa
     6601 gcgatgtgga tcaccttcaa taacggcgcg atccataaaa gcaccagggt cgccttcatc
     6661 ggcattgcaa atcatatatt tctgatctgc aggagttttg tgagccagtc tccatttggt
     6721 tcctgtagga aatccaccgc cgccgcgtcc acgaaggcca cttttttcaa tggtatcaat
     6781 cacatcttcg ccactcatgg ttttgagcgc tttgataagg ccggcataac caccattcat
     6841 gatgtattca tccatgctga ccgggtcgat gacgccacaa ttcttaagaa ccagacgttt
     6901 ttgttttaca aagaaaggat gttcctgaat atttctgata ttatcccagg gtttgagatc
     6961 ggcagtaata aactgcccca aaacattttg ctgatctata tttccggaca ttacctgttc
     7021 gaaaacacca ttgactttat cagcagtaac tttagaaaaa gagattctgt ttttgcccgg
     7081 gagtttaata tccataatcg gttctacagc gcagaaaccg atgcaaccga cttcgatgat
     7141 ttcgccgtcg atattgttgt ttttcagata ttcttcaata gcagcttttg ttttggctgc
     7201 accagcacca aggccacagg taccggcacc aataaaaaca atggtttttt tgctgtcggt
     7261 ttctttttct ggtaatgcta ccgaaattcc ctttgcattg cttgcggcaa ggttctccag
     7321 atattttttg attgagttat tcatgtctgt tattcttttg cgcggtaagt ttcaatgatt
     7381 tccttaatct tttccgttgt caccttggca aagaattcgc cgttgatgca aattacggga
     7441 gcaagaccgc aagcgccaat acacgcaact acttcaatgc tgaaaagtcc gtcgcgcgtg
     7501 gtttgtccgg ccttgatttt caaaatcgat tccagttcct gcagtactgt ggcagacccc
     7561 agtacatggc aagccgtacc acgacaaacc tgaatgtggt atctgcccac cggttcaaac
     7621 ctgaactggt tgtaaaatgt agcaacacca tagatcttgc tggttggtac ctttaattta
     7681 tttcctgttt taacaatggc ttcttcagaa agatatcctt ccttttcctg aatttcctgt
     7741 aacagcggaa tgagtgaatc gcgtccgcag ttttcgtagt ttgccagaat tttgtcaatt
     7801 gtcgtttcca tatccatgtg tgttgctaca ttatttggtt tagtgattag ccgagaatac
     7861 gtttgatttc cggaacaact tttttcgcat caaccggttt tcttacaaat ccgtcaaccg
     7921 ggaaccagat tgatcttccg tcttcagaca gatagtcaac accagctttt cccgaattga
     7981 tgtctttcac aagaatcaca tgcagatcct tgaaaccggg gtttttgcgg aattcgcgtg
     8041 ccatggcctg cacgtcgggc ttggtggtgg tcagaatgtc aatcgatgtc tgcatcagaa
     8101 ccggcatttt gctcagttcg gcatcgtcgg tgatttcttt ggccagttca aagccttcgt
     8161 aatgtgtggt catcatgaca tcaagaatgg cgagatcggg tttttcttcc ctgatttttt
     8221 tcatgccttc tttcttgtca ttggctgaaa tgacggtgta accttctttt ttaagaatgg
     8281 tctcgatgat ggtgattaca tcgatgtcat cgtctacgat taaaattttc ttggtttcca
     8341 taactgtttt tttttgaggt tagttaaaac ttcttggcgc cgggaaaaat tccggcttcg
     8401 gggttattta ttatttgaga aagcaaatca tagttatcgc ccgatttggt cagatatccg
     8461 catgctccgg cttcaaccat tctgttcaca taatcctcgt tttcatagga tgagaatccg
     8521 atgatgtgca tctccggatg actcattcgc gccttgcgtg tggcctcaac tccgtcgagt
     8581 accggcattt ttacatccat gaaaacaatg tcggcactgg tttcatcgat cagcttcaga
     8641 aactcttctc cgtttgaagc ttcgcccacc acttccacat tacctatatt ggtaaggatt
     8701 gcttttaatc ctttgcggaa ggttggttca ttatcgacta ttattacccg tgtcattgtt
     8761 gtttgtattg acgtaacaaa attagtttga gttcggggtc tggaaaagag taaaacccgc
     8821 atctgaacaa tcaggtaaaa ttacctgtat caaaacaata cactcgtact aaaaatatgt
     8881 tttcatagtc gaattaaatg ttttttaaga caaatgaaaa acaagctgat tcaacaataa
     8941 ttataaaatg aaccacaaat ggccagattt catagaaata aactcaaaaa tattttcatt
     9001 acaggtattt ttacggggta gaaaaacgga ctttttgcgg agaattccgg gaatgattgg
     9061 agcacaacgc atggagctta aaagcttaag gtttaatgct taaagactga acaaattctt
     9121 ttgctttttt tactttaaac tttaagttgt gttttacttt cactctgttt ttcgatcatt
     9181 gaaagccgct cccatagcca gaagccggaa ctaaccgact ctgccaggac acagtcgtga
     9241 ctctggcagg tcgggggtg
//
LOCUS       DAOPKE010000105         9048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8119_length_9048_cov_15.337707, whole genome shotgun sequence.
ACCESSION   DAOPKE010000105 DAOPKE010000000
VERSION     DAOPKE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8119_length_9048_cov_15.337707"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1055)
                     /gene="dapA"
                     /locus_tag="PKY63_10935"
     CDS             complement(150..1055)
                     /gene="dapA"
                     /locus_tag="PKY63_10935"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPF01173.1"
                     /translation="MKFSVQGCGVALVTPFDEQGAIDFQSMIKLVDHVVDGGVDYIVL
                     FGTTGESPTVSLQEKQEALQLVSKHLNGKVPIVIGFGNNNTARLIEEMNLFDFTGASA
                     ILSVLPYYNKPNQDGMYMHYKMIAEHAPLPVVLYNVPGRTVVNMEPATTIRIANDCPN
                     VMAIKEATDNMDQVMDLLYEKPASLEVVAGDDALALPIISLGACGVISVIANAYPKEF
                     SHMVHATMKGNMDEARRYHNLLLPIMHTLLKMGNPSGIKAVLHIKGLIQHVFRLPVHP
                     VNENKFAEIASVVKQTEKNCRQATS"
     gene            complement(1052..1600)
                     /locus_tag="PKY63_10940"
     CDS             complement(1052..1600)
                     /locus_tag="PKY63_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01174.1"
                     /translation="MLKKFRDNKFQKALSRRKADLKRHVINKSFEDSVSFLLLFDCTT
                     EEKYNEFTRIQSSLQLQKKKVHAIGFHGLPALPSWCNKSLHVAFIGKNDMSSSGIPSD
                     ELVDEFLNVPFDTLIDLTYCMHPVLHWIIVLSQAQLKIGAGEKKIQQIFDIAIEILPD
                     HTQEELFSMALHFQSMFQKKQI"
     gene            complement(1641..4877)
                     /locus_tag="PKY63_10945"
     CDS             complement(1641..4877)
                     /locus_tag="PKY63_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPF01175.1"
                     /translation="MKYTVLIAIMIMMTHTAKSQQEARLLRFPAVSANSIVFTYAGDL
                     YSVGKTGGVARRITSGDGYEMFPRFSPDGKWIAFTGQYDGNTEVYLIPADGGIPKRLT
                     HTATLGRDDISDRMGPNNIVMGWTPDGKKIIYRSRGTSFNDFIGKLYAVSVDGGLSEE
                     LPLSTGGFCSYNSDGSKLAFNRVFREFRTWKYYQGGMADDIRIFDSKTKSIEKITDTQ
                     AQDIMPMWIGNDIYFISDRDRTMNLFCYNTESKTTTKVTTFTEYDVKFPSHNNGEIVF
                     ENGGYIYLFNATTKKSGKVKILISNDMAYSRNFLADASKNIQAVSPAPGGERLVVSAR
                     GEVFSVPAKSGVTRNLTKKSSSHDRNAVWSPDGKTIAFISDRSGEFEIWTVAQDGSSE
                     PVQITKKADTYYFSIEWSPDSKKLLFSDKKLRLRYVDVETKKVNEVASNGVWEYTDFA
                     WSPDSKWIVFTEPQKSGMNQIMLFELATGKKTPVTDTWYESYNAQFSRDGKYLVFISD
                     RDFNPIYSHTEWNTAYTNMARVYLVPLDAKTPNPLAPKNDEVVFEAEKKNEPKPAEGA
                     AVSVVVTTEGIQERIVSLPIEASNYYGVEMTGDDVYYMKRGTGDDKTTLVLYSIDDSK
                     ETTIGEFDGFQITGNGKKMMLRKAKNYYMVDLPKAELKPEKNISFDQLKTTVNRQEEW
                     AQIYFEVYRQMRDFFYVENMHGVDWNAMRDKYAALLPYVNNRHDLNYIIGELIGELNV
                     GHAYISGGDCPMPKRVNTGLLGAVITTDPSGFFKVTSILKGANWSKELRSPLTEHGVN
                     VKEGEYIIAIDGVKTNTVKDMYALLAGKADVQVEMLVNSKPSADGARKVIIVPLTSEA
                     SLYYYNWVQHNVELVNKATNGEVGYIHVPDMSVEGLNEFVKYFYPQLNKKALIIDDRG
                     NGGGNVSPMIMERLMRIPTRANMARNVIVPGQTPTKLMLGPKVLLLNQYSASDGDLFP
                     YAFKKHQLGKTIGMRSWGGVVGIRGSLPFVDGTVLNKPEFASYSIEESKWIIEGYGVE
                     PDIIVDNDSYLEYTGTDTQLLKAIEIIKEELKKYTPIPEIPEAPDKSK"
     gene            complement(5046..6323)
                     /locus_tag="PKY63_10950"
     CDS             complement(5046..6323)
                     /locus_tag="PKY63_10950"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HPF01176.1"
                     /translation="MSKKDILDLAMKTNFMKRKPRKIDPCNFLAMVCTLSVLDTPSFN
                     SIAASYSVNYSILASKQAISKKIKDECLDFFKSILALIIQTRANIDEAGTTPSFLNKY
                     KRVLVQDSTIIKLPERLFTSFSGVNNAYKPVCNARIQGVYDLISGQFLSFSIDPYSKN
                     DLLAAPELEIRKDDLTIRDRGYLCQDEIQRHSDAEADCIYRYKTRTTLIDPQTGQEID
                     IYKLLKKKGYIDMKVCLNNDSRTIIRLIAAPVNEAVANRRRAKAKKETKGHNPSKKVL
                     ELMSWTIFITTIPAEMASFKKIFDIYSLRWRIEIIFKAWKSHAAFGKVHNVSEIQLRI
                     LLMARLIMVVVFTHYVYYPYASIIRSKFKREISLLKLFRFITNNLERVELLLFSECSK
                     NRNVAETNLSKYCTYDKRARLNFNQIYLEITLS"
     gene            6435..6632
                     /locus_tag="PKY63_10955"
     CDS             6435..6632
                     /locus_tag="PKY63_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01177.1"
                     /translation="MGYKVKIQRVERGSTKSFYVNFPAAVAEASNIEKGEEMEWCIHD
                     RNTFILRRIDKAKSLISKKKQ"
     gene            complement(6671..7906)
                     /locus_tag="PKY63_10960"
     CDS             complement(6671..7906)
                     /locus_tag="PKY63_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPF01178.1"
                     /translation="MARKQWIPWMAFRFWRSKKSFSIINIISGISFWGIAVGTAALII
                     VLSAFNGLEGMISTMFNNFHSELRIEPVSGKYFQNNRIDTSRIALLSGVEHVSYVVED
                     ICMIRYGDRQQVVYIKGVEPGKGFEQKFKPLMLNGSSNLGTDSVPYALVGAGIFYSLG
                     ININDFTTPLTFYSPKRTSAASADISSSFRSRSAIAGGMFSVQQEFDEKYIVVPLSLA
                     RDLLEYETISTAVEISISNESLIPGIEDELQDMAGDEFNVKNRMEQEEALYKIMKSEK
                     LIVFLILALILLITSFTIISTLTLEILSKQKDLSTLYALGAGVQQIRRVIISHGFIIC
                     LTGMMVGFLISIIVIVLQQNFGLVRFAGGDTFISDIYPVKIIWTDFVYVFFTVLAIAA
                     FISRIPVRRINAGWFSFRY"
     gene            complement(7909..8247)
                     /gene="rbfA"
                     /locus_tag="PKY63_10965"
     CDS             complement(7909..8247)
                     /gene="rbfA"
                     /locus_tag="PKY63_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPF01179.1"
                     /translation="MDSIRQQKAARLIQKELAEIFLLDGKNMFGNAMITVTIVRLSPD
                     MSIARVYLSLFGVKNKESLFQDIQIYTSEIRKLLGQRIGKQVRIIPELKFFIDDSLDY
                     MEKIDNALKS"
     gene            complement(8606..8929)
                     /locus_tag="PKY63_10970"
     CDS             complement(8606..8929)
                     /locus_tag="PKY63_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01180.1"
                     /translation="MLLLNIFMKYFLTIVAIALAVLFVWIMSRLSKNRNDFFDSVDND
                     FPEMKNLSLFSYKQHSKTNSEFGNLYEKHKTIRNKILSKWFLIIIVTMVVIILAGIIS
                     SHYSS"
BASE COUNT         2507 a         1857 c         1870 g         2814 t
ORIGIN      
        1 aatgaaaaaa tgatttcgcc ccggtcctgt aaaatttgtg ttgcttgttg ttgttgagtc
       61 tgtgcaaaaa tgaaaacaga tagcaataac agtaaaattg aaaaaaccaa tttcatggga
      121 gtgatgtttt ttctaagaca ttgaaattgc tatgaagttg cctgcctgca gtttttttct
      181 gtttgtttca caacgcttgc aatttcagca aatttgtttt cattgacggg gtgcaccgga
      241 agtctgaaaa catgttgaat gagtcctttt atatggagaa cggctttgat accgcttggg
      301 ttccccattt tgagcagtgt gtgcatgatg ggcagcagga ggttgtgata tctgcgcgct
      361 tcatccatat tgcctttcat ggttgcatga accatatgtg aaaattcttt tggataagca
      421 ttggcaataa ccgagataac tccacaggca cccagtgaaa tgatgggaag agccaacgca
      481 tcgtctccag cgacaacttc gagtgatgct ggtttttcat acagcagatc catcacctga
      541 tccatattgt ctgttgcttc tttgatggcc attacattgg gacaatcatt ggcaatgcgt
      601 atggtggttg ccggctccat attgacgacg gtacgaccgg gaacattgta taaaacaacg
      661 ggcaggggag catgttcagc aatcatttta taatgcatgt acataccgtc ctgattgggt
      721 ttattgtagt atggcaaaac agaaagtata gccgaagccc cggtaaaatc aaagagattc
      781 atttcttcaa tcagtctggc cgtattgtta tttccaaagc caattacaat gggcacttta
      841 ccgttcagat gctttgatac cagttgaagt gcttcctgtt tttcctgcag cgaaactgtc
      901 ggagattcgc ccgtagtgcc aaaaagcaca atataatcca cgccgccgtc aaccacatgg
      961 tcaacgagtt taatcatgct ttgaaaatct attgcgcctt gttcatcaaa aggagtgacc
     1021 agtgccactc cgcaaccttg tactgaaaat ttcatatttg ttttttttga aacatactct
     1081 gaaaatgcag agccattgaa aataattcct cctgtgtatg atctggcaaa atttcaatgg
     1141 caatatcaaa tatttgctgg atttttttct cgcctgcacc aattttcagt tgcgcctgcg
     1201 acaaaacaat aatccagtgt aaaaccggat gcatgcaata ggtgagatca atcagagtat
     1261 caaacggaac attcagaaat tcatcaacca actcatcgga tggaattccg gatgaactca
     1321 tatcattttt cccaatgaaa gcaacatgca atgatttgtt gcaccaggaa ggcagagccg
     1381 gtaagccatg aaaacctata gcatgaacct tttttttctg aagctgcagc gatgattgta
     1441 tccgggtgaa ttcgttgtat ttttcttctg tggtacaatc aaacaacaat aaaaaagaca
     1501 ctgagtcttc gaagctcttg ttaattacat gtcgcttcaa atcagctttg cgccggctca
     1561 gtgctttttg gaatttattg tcccggaatt ttttaagcat cgccatgttg tttttgcttt
     1621 ataacgaaaa agctgcttac ttattttgat ttatcaggtg cttccggaat ctccgggatt
     1681 ggagtgtatt ttttgagctc ttccttgata atttcaatgg ctttcagcag ttgagtatca
     1741 gttccggtat attcaagata agaatcattg tcaacaatga tatctggttc tactccatac
     1801 ccttcaataa tccatttgct ttcttcgatg gaataactgg caaattcggg tttattcaga
     1861 actgttccgt caacaaatgg caacgatccg cggataccga caacaccgcc ccaactgcgc
     1921 attccaatgg ttttaccaag ctgatgtttt ttgaatgcat aggggaaaag gtcgccgtcc
     1981 gatgctgaat actgattgag cagaagcact ttgggcccca gcatgagttt tgtcggagtc
     2041 tgaccgggaa cgattacatt gcgcgccatg ttggctcttg taggaatccg catcaagcgt
     2101 tccataatca tgggtgaaac attgccgcct ccgtttccgc ggtcatcaat aatcagcgct
     2161 tttttattca gttgcggata aaaatatttt acaaattcat tcaatccttc aacactcata
     2221 tccggaacat gaatgtatcc tacttcaccg ttggtggctt tgttaacaag ctctacattg
     2281 tgctgaaccc agttgtaata atacaacgac gcctcagagg taagcggaac aataatcact
     2341 tttcgggctc cgtcggctga tggtttgctg ttgaccagca tctctacctg cacatcggct
     2401 tttccggcca gtaatgcata catgtctttt actgtatttg tttttacacc atcaatggca
     2461 ataatgtatt cgccttcttt cacattgaca ccatgctctg tgagcgggct gcgcagctcc
     2521 ttgctccagt tggcgccctt cagaatggat gtaactttga aaaatcctga tgggtctgtt
     2581 gtaatcacag cacccagaag tcctgtattg acgcgctttg gcataggaca gtcgcctccg
     2641 ctgatgtatg catgtcctac attcagttcg ccaataagct caccaataat ataattgagg
     2701 tcgtggcgat tgtttacata gggcagtaat gctgcgtatt tgtcccgcat agcattccaa
     2761 tcgactccgt gcatgttttc aacataaaag aaatcacgca tctgacgata gacttcaaaa
     2821 taaatctgag cccattcttc ctgacggttc actgtggttt tcaactgatc gaatgaaatg
     2881 tttttttccg gcttcagttc tgctttcggg agatcaacca tgtaataatt tttggctttc
     2941 cgaagcatca ttttcttgcc gttgcctgta atctgaaatc catcaaattc gccaatggtt
     3001 gtttctttgg agtcatcaat gctgtaaaga acaagcgttg ttttatcatc accggtgccg
     3061 cgcttcatat aataaacatc atcgccggtc atttcaacac cgtaataatt ggatgcttca
     3121 atgggcaaag aaacaatgcg ctcctgaatg ccttcagttg taacgacaac actgacagca
     3181 gcaccctcag cgggcttcgg ctcgtttttc ttttcagctt caaaaacaac ttcgtcattt
     3241 tttggtgcca gcgggttggg tgtttttgca tcgagaggaa caagataaac acgtgccata
     3301 ttggtatatg ctgtgttcca ttcagtatga ctgtaaatgg gattgaaatc cctgtcgctg
     3361 atgaaaacaa gatatttccc gtcgcggctg aattgtgcat tgtacgattc ataccaggtg
     3421 tctgttactg gggttttctt tcctgtagcc agctcgaaga gcatgatttg attcatgccg
     3481 gatttttgtg gctctgtaaa aacgatccat ttgctatcgg gactccatgc aaaatcagtg
     3541 tattcccaaa cgccgttgga tgcgacttcg ttaacctttt ttgtttctac atcaacatag
     3601 cgcagacgca gttttttgtc actgaataaa agttttttgc tgtcgggcga ccactcgatt
     3661 gaaaaataat aggtgtcggc cttttttgtt atttgcactg gttcagaact cccatcctgt
     3721 gcaacagtcc agatttcgaa ctcaccgctg cgatcgctga taaaggcgat ggtttttcca
     3781 tcaggcgacc agactgcatt gcggtcgtgt gaagatgatt tttttgtcag attacgggta
     3841 acaccacttt tggccggaac agaaaaaact tcgcctcggg cgctcactac aagccgttct
     3901 ccgccgggtg caggactcac tgcctgaatg ttttttgatg catcggccag aaaattacgg
     3961 ctgtaggcca tatcgttaga aatcagaatt tttacttttc ccgatttttt tgttgttgca
     4021 ttaaaaaggt agatgtagcc accgttttca aaaacaattt cgccattgtt gtgcgaagga
     4081 aattttacat cgtattcggt aaaagttgta acctttgttg tagtcttgct ttcagtattg
     4141 taacagaaaa gattcatggt gcggtcgcga tccgaaataa aataaatatc attaccgatc
     4201 cacatgggca tgatgtcctg cgcctgtgta tctgtgattt tttcaatgga ttttgtttta
     4261 gaatcaaaaa tacgaatatc atcggccatt ccaccctgat aatatttcca ggtgcggaat
     4321 tcacggaaaa cgcgattgaa tgcgagtttg cttccatcgc tgttgtaact gcagaagccc
     4381 ccggtagaaa gtggcagctc ttcagaaagt ccgccatcaa cagaaacagc atataatttt
     4441 ccgatgaaat cattgaatga tgttccgcgt gagcggtata tgattttctt tccatcgggg
     4501 gtccatccca ttacaatatt gttgggaccc atgcggtccg aaatgtcgtc tctgccaagc
     4561 gttgcagtat gtgtgagtct ttttgggatg ccaccatcgg ccggaatcag atatacttcg
     4621 gtgttgccgt cgtattgtcc ggtaaaagca atccactttc cgtcaggtga aaaacgcgga
     4681 aacatttcat atccgtcacc ggatgtgatg cggcgtgcaa cgccaccggt tttcccaaca
     4741 gaatacaaat caccggcata ggtgaaaaca atggaatttg cgctgactgc agggaaacgc
     4801 agaaggcgtg cttcctgctg gcttttggct gtgtgtgtca tcatgatcat gatagcgata
     4861 agaacagtgt atttcatttt ctgcaattat tttttgaaag ttcaaaagta cggatttttg
     4921 ccgagaatga acagcatcag aggctgatta ttatcagctg ccttctgggc gattgcatcc
     4981 ttttccctgc agctattctg gtttacaagc cctcgaaggg ttttgaaccc ttcgaggcat
     5041 acgcgtcaac ttaaagtaat ttcaaggtat atctgattga agttcaatcg tgcacgttta
     5101 tcatatgtgc agtattttga tagattcgtt tctgcgacgt tgcgattttt gctgcactca
     5161 ctaaataata atagttcaac gcgttcaaga ttattagtaa tgaagcgaaa taatttgagc
     5221 aagctgattt ctcgtttgaa tttagacctt attatacttg catatggata ataaacgtaa
     5281 tgggtaaata caacaaccat tattaatctt gccataagta atatgcgcaa ctgaatttca
     5341 gaaacattgt gtactttacc gaatgcagcg tgacttttcc aggccttgaa gatgatttca
     5401 attctccagc gaagagaata tatatcaaaa atttttttga agcttgccat ttctgctgga
     5461 atcgtagtga tgaaaattgt ccaggacatt agctcaagca ctttttttga agggttatga
     5521 ccttttgtct ctttctttgc cttagccctt cgtctgtttg ctactgcttc gttcacgggg
     5581 gctgcgatta gtctgattat tgttcggctg tcgttgttca aacacacttt catatcaata
     5641 tatcctttct ttttcagcag tttgtaaata tcaatttctt gtcctgtttg agggtctatc
     5701 aatgttgtcc ttgttttgta tctgtaaata cagtcggctt ctgcatccga atgccgttgt
     5761 atttcatcct gacataaata tccacgatct cttattgtca gatcatcttt gcgtatctcc
     5821 aattccggag ccgccaacaa atcatttttc gaataaggat ctattgaaaa tgagagaaat
     5881 tggccggaga taagatcata tacgccttgt atgcgggcgt tgcagactgg tttgtatgca
     5941 ttatttacac ctgaaaatga agtaaacagc cgttcgggaa gtttgattat agtactatcc
     6001 tgaactaaaa cacgtttata tttatttaaa aaggaaggcg tagtgccagc ttcatctatg
     6061 ttcgcccttg tttggattat cagagcaaga attgatttga aaaaatcaag acattcatct
     6121 tttattttct tgctaatggc ttgtttactt gccagaattg agtagttgac tgaatagctc
     6181 gcagcaatac tgttaaagct tggagtatca agcactgaaa gggtgcagac cattgccaga
     6241 aagttgcatg gatcaatttt ccggggcttt cgcttcatga aatttgtttt cattgccaga
     6301 tcaagaatgt cctttttaga aattccttca acatcaatta tgtcgaaaaa acttttttga
     6361 gatttttttt catgatatca gatttttgtt tacttttgca atagtataaa tactattaat
     6421 aaaaaccaaa caaaatgggg tacaaagtca aaattcaaag agttgaaaga gggagcacaa
     6481 aatccttcta tgtaaacttt cctgccgcgg ttgcagaagc ttcaaacatt gaaaagggag
     6541 aagaaatgga atggtgtatt cacgacagga atactttcat tctaagaaga attgacaaag
     6601 ccaaatcgct tatcagcaaa aagaaacaat aattaagttg acgcgtatgc cttcgagggc
     6661 ttaaacattt ttaatacctg aagctgaacc atccggcatt gattcgcctg acgggaattc
     6721 gtgaaataaa cgctgctatg gcaagcacag taaaaaatac ataaacaaaa tcggtccata
     6781 tgattttgac cggataaata tcgctgatga aagtatcacc tccggcaaac cgcaccagac
     6841 caaagttttg ctgaagtaca ataacaataa tgctgattaa aaacccaacc atcattcctg
     6901 taagacaaat aataaaacca tgcgaaataa tgacccgtct gatttgttga acaccggcgc
     6961 ccaaagcata aagtgtcgag aggtcttttt gtttgctcag gatttcaaga gttagtgttg
     7021 aaatgatggt gaatgatgtg atgagcagaa tgagtgcgag aatcagaaat acaatcagct
     7081 tctcgctttt catgattttg tatagtgcct cttcctgctc cattctgttt ttcacgttga
     7141 attcgtcacc ggccatatcc tgcagctcat cctcaatacc gggaatcaga ctttcattgc
     7201 tgatgctgat ttccacggca gtggaaatgg tttcgtattc cagcaaatcc cgggcaagcg
     7261 atagaggaac gacaatgtat ttttcatcaa attcctgttg aaccgaaaac atgcctccgg
     7321 caatggcgct tctggagcgg aacgacgatg atatgtcggc cgacgctgcc gatgttcttt
     7381 tcgggctgta gaatgtgagc ggcgttgtga aatcattgat attgatacca agggaataaa
     7441 atattcctgc accaacaagc gcatagggca cactgtctgt gccaaggttg ctgctgccgt
     7501 tcagcatgag cggtttgaat ttctgttcga aaccttttcc gggttccact cctttgatat
     7561 ataccacttg ctgccggtcg ccgtaacgaa tcatgcagat atcttccacc acataactga
     7621 catgctcaac accgctcagc aaggcaatcc gggaagtatc aatcctgttg ttctgaaaat
     7681 attttccgct gaccggctca atgcgcagtt cgctgtgaaa gttattgaac attgtgctga
     7741 tcatgccttc cagtccgttg aacgcagaaa gcactataat cagcgctgct gttccaacag
     7801 caatgcccca gaatgaaatg cctgagatga tgttgatgat gctgaacgat tttttcgatc
     7861 gccagaaacg aaatgccatc cagggtatcc attgttttcg ggccacagct atgattttag
     7921 cgcgttgtca attttttcca tgtaatccag actgtcgtcg atgaaaaact ttagttcagg
     7981 aataatgcgt acctgtttgc caatgcgctg tccaagaagt ttacgaattt cagatgtata
     8041 aatctggata tcctgaaaca atgattcttt gtttttaaca ccgaagagac tcagatacac
     8101 tctggcaata ctcatatccg ggcttagccg cactatggtg actgtaatca tggcgttgcc
     8161 gaacatgttt tttccgtcca gcaaaaatat ttcagccagt tccttttgaa tcagccgggc
     8221 tgctttttgt tgtctgatgc tatccatgat gcaaaaatac gatttttaat ttgctgatgt
     8281 actaatgtga aaatgtgggt ctttgtccat gtgggaatat gagcgacgtt attattggct
     8341 gatgtgctaa tttgataatt ggctgatttg ctaatgattt gaaattttga tgatttattc
     8401 acccattaaa ttattttcaa aggagttcat cacgtgaaac agcttgccac gttccacgtg
     8461 gtgacaagtc gagctcacga tttcatcgtt cggttgaaaa tttgaaaatg taagatttag
     8521 aatgtgggaa tttagatacc tataaaataa cacgaataag aagctttgtt ttggatagat
     8581 aagtgattca aagttccatg taatattaag agctgtagtg agagctaata atgccggcaa
     8641 gaataattac aaccattgtt acaataatta tcaaaaacca ttttgaaagt attttatttc
     8701 ggatagtctt gtgcttttcg taaagatttc cgaattcaga attggttttg ctgtgttgtt
     8761 tgtaagaaaa aagactgaga tttttcattt ccggaaaatc attatcaacg ctatcaaaaa
     8821 aatcatttcg atttttactc aatctgctca ttatccatac aaataagaca gcaagcgcta
     8881 ttgcaacaat agtcagaaaa tacttcatga aaatattcag taacagcatt tgtagggact
     8941 aaagattttc tgtatccata acctcccgat actccttaat aagattatca aggaacttct
     9001 ctgaagaaaa gtcgagtgcg cgttttcggg cttcctgagc cagttgtg
//
LOCUS       DAOPKE010000106         8952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8230_length_8952_cov_10.639991, whole genome shotgun sequence.
ACCESSION   DAOPKE010000106 DAOPKE010000000
VERSION     DAOPKE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_8952_cov_10.639991"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..808)
                     /locus_tag="PKY63_10975"
     CDS             complement(53..808)
                     /locus_tag="PKY63_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPF01181.1"
                     /translation="MNTFRVFDTLVAMRKEPSHRSEMVNELLYGEDIIITNRHNGWLQ
                     IKCAEYDYEGFIPESVQLDEISIKTNEPYRFVFTASSMIRSKDGIKLDLPRGSRVRNE
                     KTERGCFETPDKINSISEIVKTAGFYLGNPYRWGGRTPFGIDCSGYTQMVFMLNRLII
                     PRDANQQVLLGDEVAFVSEAQPGDLAFFQNENGEVIHVGILLSNAEIIHASGHVRIDN
                     IDHHGIFNKELNQYTHTLKVIKRMSVDALPSCM"
     gene            complement(786..1454)
                     /locus_tag="PKY63_10980"
     CDS             complement(786..1454)
                     /locus_tag="PKY63_10980"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPF01182.1"
                     /translation="MQFCIFAIMSNQSKKILIPSLLAPPAKLFALIHSCDEVIIDIHE
                     TYPKQTLRNHYHIGGPNDVRPLVVPVHKPKGHRSTSYEVEIDYSENWPLYHKKTLTTA
                     YSKSPFFLFYADAIFAEFEKKHQFLIDLNKALRNIFWKWLFIDTPITMADGFGDYNHC
                     TDLRHAFKKSTDWTLQRPYYQPFDTKYGFRNSLSVLDIIFNLGPETPEFLKTENTDEY
                     FQSF"
     gene            complement(1776..2156)
                     /locus_tag="PKY63_10985"
     CDS             complement(1776..2156)
                     /locus_tag="PKY63_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HPF01183.1"
                     /translation="MVAEITMKLAWSEFIWSDKIWIIIGAVFMILGLLGCFVKKIPGT
                     PFGLAGLLMLQLMNEPPFYTFQILILSAITLVVISVDYVAPAIAEKQFGKTKPGIIIS
                     NIVKFAVVGYMAYLFVMAIMQAHA"
     gene            complement(2301..2915)
                     /locus_tag="PKY63_10990"
     CDS             complement(2301..2915)
                     /locus_tag="PKY63_10990"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HPF01184.1"
                     /translation="MVKYLSTCILFFGLIIVSSPTNAQEENDKQPLINIKTNPFASML
                     SMIPLSIETFITHNLSASANLYIVSSNNGASETYFEQSGFGISPEIRYYFTDNTVGGQ
                     KNRVYAATSFSYEEGTNTTLDRYDDPIPGWTHALGGALMVGNQWFFRNRFTVDFFLGP
                     AYMSYVKNENYDANLSKGGFFLGMLGSKASGTRIKFGFNIGIAF"
     gene            complement(2909..3784)
                     /locus_tag="PKY63_10995"
     CDS             complement(2909..3784)
                     /locus_tag="PKY63_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HPF01185.1"
                     /translation="MKTALVTGIALLIFASLSAQTTIPNAGFEDWTAEGSYENPNSWD
                     SPNDVTSSLGIVVVEKEEVYVHSGNYAAMITVKSSIAGNMPGVLTLGDFSFDLLSMTA
                     TIEGGIPFTGKPEKISGWYQYDPNNNDICLIGAFLLQDNGGTWDTIATAGFESSATVQ
                     TWTQFEADFDYRNTNNPTHLNIILMPTDRNNPQSGSTIYIDDLELSYTQSILEKNQNL
                     VKSYSDYDANMVKISFPEVSKASVLLMDINGKKLKEIQISGNEASLEMKGISNGIYVL
                     QIADEFNIYSTKIIW"
     gene            complement(3789..4142)
                     /gene="trxA"
                     /locus_tag="PKY63_11000"
     CDS             complement(3789..4142)
                     /gene="trxA"
                     /locus_tag="PKY63_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF01186.1"
                     /translation="MEHLTNETFKEKVFNYEVNKEWKYEGQLPCIIDFYADWCGPCKM
                     VAPILYELSKEYEGKINIYKVNTEEQQELAAAFGIQSIPSILFCPMKEQPQMAMGALP
                     KPTFVQAINDILLKQ"
     gene            complement(4155..4685)
                     /gene="trxA"
                     /locus_tag="PKY63_11005"
     CDS             complement(4155..4685)
                     /gene="trxA"
                     /locus_tag="PKY63_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF01187.1"
                     /translation="MKILHFASVAFLALVVISCSGKKESKNNESVNSSTEQTTEETTT
                     EKPASGFTTEGSGQVVYLNTQNFADYIFDFRNEKEWKFKSNTPCVVDFYADWCKPCKM
                     VAPIMEELAAEYKGNIQFYKVNTDFEQELANAFGIQSIPSIMMCPKNGKPVMTAGALP
                     KSEYVRMIQEIILNAN"
     gene            complement(4787..6892)
                     /locus_tag="PKY63_11010"
     CDS             complement(4787..6892)
                     /locus_tag="PKY63_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPF01188.1"
                     /translation="MKNTVLFMALLLFSTLLSAQGIEYPQTHRDEVTDDYFGTQVADP
                     YRWLEDENSEETKSWVEKQNAVTNQYLNDIAFRDQIRERLQKLWNYEKSGIPFKKGPW
                     VFCFRNTGLQNQSILYIRNGYEGTERLLLDPNKLSEDGTTALSGYSISKDYKYMAFST
                     SKGGSDWNDIQVMEIKSGKMLSDVVKWTKFSGIAWYKDGFFYSAYDVKDESKALSTKN
                     EFHKVYYHKLGTSQAKDILVYENKEYPLRNYGIYLDEDEKYMFLSESESTSGNALWVS
                     PARTTDIKWTKIADGFEFDYNVIDVIDEKLYVLTNDGAPNNRLISIDPTDPAVSTWRD
                     IIPENENVLQSVSHINGKLVVNYLEHASDRLYIFTTNGKKEKMIGLPGIGTIGGPSGN
                     RDESVFFYSYTSYTMPNSIFRYDMETGNNEKYWSPKVDFNPEEFITKQVFFESKDGTK
                     IPMFLTYRYDTKMDGERPVLLYGYGGFNISLTPGFRLTAIPFLEAGGIYAVVNLRGGG
                     EYGKKWHEAGTKHQKQNVFDDFIAAAEYLIENSYTNSSKIAIQGGSNGGLLVGACMTQ
                     RPELFKVALPAVGVMDMLRFQKFTIGWAWTGDYGSSDNKEDFEYIYKYSPLHNINKGV
                     NYPATMVTTADHDDRVVPAHSYKFISELQYKHEGDNPVLIRIETMAGHGAGKPTSKQI
                     DEAADILSFMMHNLGMKLK"
     gene            6943..7845
                     /locus_tag="PKY63_11015"
     CDS             6943..7845
                     /locus_tag="PKY63_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01189.1"
                     /translation="MNRKIKSLRVTRHQQYLDRFDSDELKELVFLAEEQLFDEAGRVI
                     TIVKYTQSGELEEKVVKTYQGNMVTTEFFIDENELSEKSVAETDDNGRMTKETMYYAD
                     GTQSVSEFEFDGDKPVKKLTMDIDEYEREISGEHIWTYDSAGNLLREEEIEYGELSFY
                     REMKYDEKNHMTELTTFHAADGRPSHEKMEWADNEVSHVIKTDIYGNKTESFYKYNAQ
                     GDAEFIRFRSREYSSETVVKYDESNNPVHELETDDKGEILYEVVREYDPETRNLTRVE
                     NFINRQGRGTNVHYVLEYEYTFYP"
     gene            8257..8910
                     /locus_tag="PKY63_11020"
     CDS             8257..8910
                     /locus_tag="PKY63_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01190.1"
                     /translation="MKNLCLSAIVLLFWGLTLSMAQPSVNPEQTNYTAQSGFVANLGQ
                     SEMLQDHLFSWKHNNVFAYFMKDRIVFLTQEIRHEENPQSAEAKAKGDENRAKRLAAK
                     TYVSRFDLVFENALSAVEIQGEDENSVQMDFYYAHCPEGLLKVPSFNNIRYKNIWQNI
                     DLVFTFDGTSLKYFFEVAPGANIHDIVLRWDGVENLELNDKGELQFNLGAFTFYGHL"
BASE COUNT         2506 a         1715 c         1853 g         2878 t
ORIGIN      
        1 tttgatgaat atatttctgt ttttgtgagt gttacgtgtt tttcagcaga ccctacatac
       61 aggatggcag tgcatcaaca gacatgcgtt tgatgacttt cagcgtgtga gtgtattggt
      121 tcagctcttt attgaaaatg ccatgatgat cgatattgtc gatgcgtaca tggccggatg
      181 catgaatgat ttctgcgtta ctgagcaata ttccgacatg aataacctct ccgttttcgt
      241 tttgaaaaaa agccagatca cccggttgag cttcagaaac aaaagccact tcatcgccca
      301 gcagcacctg ttggttggca tcgcggggga taatcaaacg attcagcata aagaccattt
      361 gagtgtaacc agagcaatct attccaaatg gagtgcggcc tccccagcga taaggatttc
      421 caaggtaaaa accggcagtt ttcacaattt cactaatgga atttatttta tccggagttt
      481 cgaaacaacc gcgttctgtc ttttcgttgc gtacccggct tccacgtgga agatcaagtt
      541 tgatcccatc tttggaccgg atcatggaag aagctgtgaa aacaaaacga tatggttcat
      601 ttgtttttat tgaaatttca tcaagttgta ccgattcagg aatgaaccct tcataatcat
      661 attcagcaca tttaatttgc aaccagccat tgtggcggtt ggtaatgata atatcttcgc
      721 catataataa ttcattcacc atttcgctgc ggtgtgatgg ttctttgcgc atggcaacca
      781 aagtatcaaa aactctgaaa gtattcatct gtgttttctg tttttaaaaa ttccggtgtt
      841 tcggggccta aattaaaaat aatatcgagg actgaaagag aatttctgaa tccgtatttt
      901 gtatcgaatg gttggtaata ggggcgctgc aacgtccaat cagtgctttt tttgaatgca
      961 tggcgcaaat ctgtacaatg attatagtcg ccaaatccat cggccattgt tataggtgtg
     1021 tcaatgaaca accatttcca aaaaatattt ctcagtgctt tattgagatc aatcagaaat
     1081 tggtgttttt tttcaaattc tgcaaaaata gcatcggcat aaaacaaaaa aaacggactt
     1141 tttgaatatg cagttgtcag tgtttttttg tgatagagtg gccagttttc gctgtaatca
     1201 atttcgactt cataggatgt gctgcggtgt cctttgggtt tgtgaaccgg cactacaagt
     1261 ggcctcacat cgttcggacc tccgatatga taatgattcc gaagtgtttg tttcggatat
     1321 gtttcgtgaa tatcaataat cacttcgtcg catgaatgaa tgagtgcaaa taattttgca
     1381 ggcggtgcca gcagcgatgg aatcaatatt ttttttgatt gattgctcat gatggcaaag
     1441 atacaaaatt gcatcagcta tcagtcgtca gcaatcagca ataggtgacg accgaagcgt
     1501 tggtgctctg agaaagagct atgcgggatt tctttatctt ataatgtgca ttgtgtctta
     1561 tgccgcattc ctcatgctcg aacgtcgcct ttaaaacttg atgaactttc atctaaaaaa
     1621 ggagcttcac gtaaaacgtg acgtggtcgc acaccttgtc cctggggcct ttggcccaag
     1681 gctaacatgt ttagcgccgt cagcgcatcc gatggtgccg cgcagctttc ctttacgcct
     1741 tcggccacaa ttacgttatg cgttgtgcta caaaatcagg cgtgcgcctg catgattgcc
     1801 atgacaaaca ggtaagccat gtatccgaca acagcgaatt tcacaatatt actgatgatg
     1861 atgcctggtt ttgtttttcc gaattgtttt tctgcaatgg ctggtgcaac ataatcaaca
     1921 ctgatgacta cgagtgtaat ggccgacaaa atcagaatct gaaaagtata gaatgggggt
     1981 tcattcatga gttgcagcat cagcagtcca gccagtccga atggtgttcc gggaattttt
     2041 tttacgaaac atccaagcaa tcccagaatc ataaaaaccg ctccgataat aatccagatt
     2101 ttatcactcc agatgaattc gctccaggcg agtttcatcg taatttcagc caccattttc
     2161 tatcaatttg gtgacaaaga tagaaaagat ttttctgtaa atatgtaatt ttaagaaagt
     2221 tgcgaatatg acacaattca attgcttagt cgtttttttg tggccttacg atagtaaaat
     2281 tcaatcagat taaatcattt tcagaaagca attccaatgt tgaatccgaa tttgattcgg
     2341 gtaccgcttg cttttgagcc gagcattcca aggaaaaatc cgcctttgga aaggttggca
     2401 tcatagtttt cgtttttcac atagctcatg taggcaggtc ccagaaaaaa atccacggta
     2461 aaacggtttc tgaaaaacca ctgatttcct accatcaatg caccacctaa ggcatgagtc
     2521 catccgggaa tcggatcatc gtagcggtcg agtgtggtat tggttccttc ttcgtaacta
     2581 aaagaagttg ctgcatatac cctgtttttt tgtccaccca cagtattgtc ggtgaaataa
     2641 tacctgattt cgggtgaaat accaaaacca ctttgttcaa aatatgtttc gctggctcca
     2701 ttgttcgaac tgacaatata tagattggca gaagcactca gattatgtgt tataaatgtt
     2761 tcaatgctca gcgggatcat gcttaacatg gatgcaaacg gatttgtttt aatgtttatc
     2821 agcggctgtt tatcattttc ttcctgagca ttggtaggcg aagagactat gatcagtccg
     2881 aaaaaaagaa tgcatgttga tagatatttt accatatgat tttagttgaa tatatattga
     2941 attcatcagc aatttgtaat acataaattc cattggaaat gcctttcatt tcaagacttg
     3001 cctcattacc tgaaatttga atttctttta atttttttcc attgatatcc atcagaagga
     3061 cactggcctt tgagacttcc gggaaagaga ttttaaccat atttgcatca taatccgaat
     3121 atgattttac caggttttga tttttttcaa gtatggattg agtatatgat aattcaaggt
     3181 catcaatgta aatggtgctt cctgactgcg gattattgcg gtctgttggc atcaggataa
     3241 tattcaaatg agttgggtta ttggtgttgc gataatcaaa atccgcttca aactgtgtcc
     3301 aagtctgaac agttgcagag ctttcaaaac ctgcagtggc tatcgtgtcc caggttcctc
     3361 cattgtcttg cagcagaaag gcaccaatca ggcaaatgtc attgttgttg gggtcatatt
     3421 gataccatcc actgattttt tctggttttc cggtaaaagg aattccgcct tcaatggtgg
     3481 ccgtcatgct tagcaggtcg aaactgaaat cgcccaatgt aagcactcca ggcatattac
     3541 ctgcaatcga tgatttaact gtaatcatag ctgcataatt gccactatga acatacactt
     3601 cttctttttc aactacaact atccccagag aggaggttac gtcatttgga ctgtcccatg
     3661 aattggggtt ttcgtatgat ccctccgctg tccagtcttc gaaaccggca ttgggaattg
     3721 ttgtttgagc agataacgat gcaaaaatca ataatgcaat gccggtaacg agagctgttt
     3781 tcatattttt attgttttaa aaggatgtcg ttgatggcct gaacaaaggt tggctttgga
     3841 agcgctccca tagccatttg tggctgttct ttcatcgggc agaacaatat gcttggaatg
     3901 ctttgaatgc caaaagcggc tgccagttcc tgttgttctt cggtgttcac tttgtaaatg
     3961 tttatttttc cttcatattc tttggaaagt tcgtatagta tgggcgccac cattttgcaa
     4021 ggaccacacc aatcggcata aaagtcaatg atgcatggaa gctgcccttc atatttccac
     4081 tctttgttta cttcgtaatt gaagactttt tctttgaacg tttcgttggt aagatgttcc
     4141 atattcgttt tggtttaatt tgcattgaga attatttcct gaatcatgcg cacatactcg
     4201 cttttgggta aggccccggc tgtcatgacg ggtttcccgt ttttggggca catcataatg
     4261 cttgggatac tttgaattcc gaaggcattg gccagttcct gctcaaagtc tgtatttact
     4321 ttgtagaact ggatgttgcc tttgtattct gctgcgagtt cttccatgat tggagctacc
     4381 attttacatg gtttgcacca gtcggcataa aaatcaacca cacagggcgt gttgctcttg
     4441 aatttccatt ctttttcatt gcggaaatca aaaatgtaat cagcaaaatt ctgggtattc
     4501 agataaacta cctgtccgct tccttcggta gtaaaacccg atgcaggttt ttccgtagtt
     4561 gtttcttcag tagtttgttc tgttgatgaa ttaacggatt cattgttttt gctttccttt
     4621 tttcctgaac aggaaataac gacaagagct aaaaaggcga ctgacgcaaa gtgtaaaatt
     4681 ttcatgctgt ttattttatg caaagttacc aacgattgct tttggttttt atgttgaaat
     4741 gatgatataa atcatccggt gattttttat tgtaaacaga tttttgttat ttcaatttca
     4801 tcccaagatt atgcatcatg aatgaaagaa tatctgctgc ttcgtctatc tgcttcgatg
     4861 ttggtttacc ggctccgtgt ccggccattg tttcaatccg gatcagtaca ggattgtctc
     4921 cctcgtgttt gtattgcagt tctgaaatga atttgtaaga atgagccgga actacgcgat
     4981 cgtcgtgatc ggccgttgta accatggttg ccggataatt cactccttta tttatattgt
     5041 gcagcgggct gtatttgtaa atatattcaa agtcttcttt gttgtcactt gagccatagt
     5101 cgcctgtcca tgcccatcca atggtgaatt tttgaaaacg cagcatatcc atcactccta
     5161 cagcgggcaa agctacttta aataattcag gacgttgagt catgcaggcg ccaaccagca
     5221 gacctccgtt ggatccccct tgtatggcta tttttgatga attggtgtaa ctgttttcaa
     5281 tcaggtattc tgcggctgca ataaaatcgt caaacacatt ttgtttctga tgttttgtgc
     5341 ctgcttcatg ccattttttt ccgtattcac ctccaccacg cagattcact actgcataaa
     5401 taccacctgc ttcgagaaac ggaatagcgg tgagacggaa acccggggtc agactgatat
     5461 tgaatccacc atatccatac agcaatacag gtcgctctcc gtccatttta gtatcatacc
     5521 gataagtgag aaacatcgga atttttgttc catccttgct ttcgaagaat acttgttttg
     5581 tgatgaattc ttcaggattg aaatcgactt taggactcca gtatttttcg ttgttgccgg
     5641 tttccatatc atatctgaaa atgctgttgg gcatggtgta gcttgtgtag gaataaaaaa
     5701 atacggattc gtcgcggttt cccgatgggc ctccaatggt gccgattccg ggtaatccga
     5761 tcattttttc ttttttcccg tttgtggtaa aaatatagag acggtcagat gcatgttcga
     5821 gataattcac aaccagtttc ccattgatat gtgaaacaga ctgaagtaca ttttcatttt
     5881 caggaataat atcgcgccaa gtgctgactg cagggtctgt cgggtcaatt gaaatcagcc
     5941 ggttattcgg cgcgccgtcg tttgtgagta catataattt ttcatctata acatcaataa
     6001 cgttgtaatc gaattcaaat ccatcggcta tctttgtcca tttgatatca gtggttctcg
     6061 caggactcac ccacagagcg tttccactgg tgctttcact ctcgctcaga aacatatatt
     6121 tttcatcttc atcgagataa atgccgtaat tacgaagcgg atattctttg ttttcataaa
     6181 ccaaaatgtc tttggcctgc gatgttccga gtttgtgata atagactttg tgaaattcat
     6241 tttttgttga aagggctttg ctttcgtctt taacatcata agccgaataa aagaagccgt
     6301 ctttatacca ggcaatgcct gaaaattttg tccattttac tacatcagaa agcattttgc
     6361 cgcttttgat ttccataacc tgaatgtcgt tccagtcgct gcctcccttc gatgttgaaa
     6421 aggccatgta tttgtaatct tttgatatcg aataaccact taaagcggtt gttccatctt
     6481 ctgatagttt gtttgggtca agcaaaagcc gttcagttcc ttcatatccg ttgcggatgt
     6541 acaaaatgct ctggttttgc aatcctgtat ttcggaagca gaacacccag ggaccttttt
     6601 taaaaggaat gcccgatttt tcatagttcc agagtttttg taatcgctct ctgatctgat
     6661 cgcgaaatgc aatatcattc agatattggt ttgtaacggc attttgtttt tcgacccagg
     6721 atttggtttc ttctgaattt tcatcctcaa gccagcgata gggatctgct acctgtgtgc
     6781 cgaaataatc atcagttact tcatcccgat gcgtttgggg atattcgatt ccctgcgctg
     6841 aaagcagtgt tgaaaaaaga aggagtgcca taaaaagaac agtatttttc atatcttgca
     6901 tttgattact tttgtattca aagataatta aaaaattatt tcatgaacag aaagattaaa
     6961 tcgcttcgcg taacccgcca tcagcaatat cttgatcgtt tcgattcgga tgagctgaaa
     7021 gagcttgttt ttttggccga agaacagctt tttgatgaag ctggccgggt tattacgatt
     7081 gtcaaatata cgcagtccgg cgagttggaa gaaaaagttg tgaaaaccta tcagggaaat
     7141 atggttacta cagagttttt tattgatgaa aatgagttga gcgaaaaatc agttgctgaa
     7201 accgacgaca acggacgaat gactaaggaa accatgtatt atgccgatgg cacacagtct
     7261 gtgagcgaat ttgaatttga cggcgataag ccagtgaaaa aactgaccat ggatattgat
     7321 gaatatgagc gtgaaatcag tggagaacat atctggacat acgactccgc cggcaatctg
     7381 cttcgcgaag aagaaattga atatggcgaa ctctcttttt atcgcgaaat gaaatatgac
     7441 gaaaaaaatc atatgacgga gcttactaca tttcatgcag cagacggccg gccctcgcac
     7501 gaaaagatgg aatgggcgga caacgaagtt tcgcatgtaa ttaaaaccga tatatacgga
     7561 aataaaaccg agtcttttta caaatacaat gctcagggtg atgcagaatt catacgtttc
     7621 agatcacggg aatattcttc cgaaacagtg gtgaaatatg acgagagcaa taatccggta
     7681 catgagcttg aaaccgatga taagggtgaa attttgtatg aagtagtcag ggaatacgat
     7741 cccgaaacaa ggaatctgac acgtgttgaa aacttcatca atcgtcaggg acgtggaacc
     7801 aatgtacact atgtgctgga atatgaatac acattctatc cgtaaaaagt agaacccgcg
     7861 ttattatcgt ttttttctga tttcagatcc ttagctttca ttattatgat atggattgaa
     7921 attttgtgtc tgccaaaata gctgtaatta catgctgttc aaataaatga ttttattcaa
     7981 acatgcatca aatcatttat tacaaaaagc agcatattca aaatccgcat tgtgtgtgaa
     8041 aaaatcattt tcatataaaa aaaatcacga tttatgttaa aatctcagat aaatgtatta
     8101 aattggccgt ctaattaata acaaaaatca ggggttatag attgaatttg tatgtgggga
     8161 ttctgaagta ttgactatga gtgcttgctg agttcatctg caaatccgga gattttgttg
     8221 ttaagtgttt aatgttaatc acttaagttt attgatatga aaaatctctg tttgtcggca
     8281 attgtattac ttttttgggg tctgacactg tcaatggcgc agccgtctgt gaatccggag
     8341 caaacaaatt atactgctca atcaggattt gtggcaaatc ttggtcaaag tgaaatgttg
     8401 caggatcatt tgttttcgtg gaagcacaac aatgtctttg cgtatttcat gaaagacaga
     8461 attgtttttc tgacacagga aatcaggcac gaagagaacc cccagtctgc agaagccaaa
     8521 gcaaaaggtg atgaaaatag ggcgaaacgt ctggctgcaa aaacatatgt gagccggttt
     8581 gaccttgttt ttgaaaatgc cttgtcagca gtcgaaattc aaggtgaaga tgaaaattct
     8641 gttcagatgg atttttatta tgctcattgt cctgaagggt tattaaaggt tccgtcattt
     8701 aataatatca gatacaaaaa catctggcag aacattgatt tggtttttac gtttgatgga
     8761 acttctttga aatatttctt tgaagtagct cccggagcga atatccatga tattgtcctt
     8821 cgctgggatg gtgttgaaaa ccttgagttg aatgataaag gagagcttca attcaatctg
     8881 ggtgcattta cattttatgg gcatctctaa aaactcaaaa accgcatcaa cactgttgat
     8941 aaaggctgtt ta
//
LOCUS       DAOPKE010000107         8882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8323_length_8882_cov_10.675314, whole genome shotgun sequence.
ACCESSION   DAOPKE010000107 DAOPKE010000000
VERSION     DAOPKE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8323_length_8882_cov_10.675314"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKY63_11025"
     CDS             <1..809
                     /locus_tag="PKY63_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01191.1"
                     /translation="AYEIDPDRREIYPDLIVYYETNGKYADKEKVLIHQQEIAPSSPG
                     MMAWNYNALATLGKNAIVLTGGDNDTYQKWTLQAVYNIRPDVRVINTSLIMIESYRNR
                     LFAETGIAPFTTAVDSSNYQTYTQLIVEHICRNSGTRPVYICNSVPEEHYASLKDSLY
                     LEGIVYKYSPERYDNIAVIRRNYEQIMLLDYIVAPISADVSRAIVVNSNLNYISAFIQ
                     LYDHYRLCGELDKAVQLASILEKIVTASDNAEYRKYVEDYLKENNDAGRE"
     gene            793..1383
                     /locus_tag="PKY63_11030"
     CDS             793..1383
                     /locus_tag="PKY63_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF01192.1"
                     /translation="MLAESRHAATLVGSDNELLERVAGADHAAFTELYNRWAGKIRYF
                     FLRLYNYDYDTADDRTQDTFLKVLENAQKFDHDQRFSPWLYAIACNLYKNDVRRRKLD
                     QHFREAAIAETTVEMPSAERKVEIQSSVKLVHQYLEKLDPEVKSIFLLRHAGELTIPE
                     IARVTGCPEGTVKSRLFYAMKKMSENLQPHTINEWI"
     gene            1380..1997
                     /locus_tag="PKY63_11035"
     CDS             1380..1997
                     /locus_tag="PKY63_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01193.1"
                     /translation="MKSADLFEDFEKLLLSKEYEQLNEAERNIIFMYCSGPQEYSAMR
                     LLMQQSQTMAAESNTMTPPSGGAEQVWARSVMQNNINEKPHGFNRTISVWWAAGLAAA
                     LILSWLIRFPAFTQKEVPMLIPDVTTRLITEVKTDTVIKEVLVYIHPETQADTCIPES
                     SNDVVSYGGMVYVQPVSSVDENKIQRQGTNASEMGDLAKMQVTVY"
     gene            complement(2017..2691)
                     /locus_tag="PKY63_11040"
     CDS             complement(2017..2691)
                     /locus_tag="PKY63_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01194.1"
                     /translation="MHFILDYRASALVENLRTYGEITLFDAGNIVYPAICGHPDIFMA
                     HINNQWILSPNAPSDITALFNELQIPFIQGNKKVGFRYPDTALYNVFCDDEIAVVSKH
                     TDESILSQLKSADIISVKQGYIACNLARIGSEFITSDAGIEKELKEKNKIIHFVKPDK
                     ITLSGVPNGFIGGTLGQSEDKVFFTGSRGSSYARLLAEIANRQNKEIIFLGKSEAVDA
                     GGIVIV"
     gene            complement(2675..3676)
                     /locus_tag="PKY63_11045"
     CDS             complement(2675..3676)
                     /locus_tag="PKY63_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF01195.1"
                     /translation="MKSHSIIPVFLPELGCPKQCVFCNQKHISGQHSVPDTDEIKNII
                     EQHLSTTKERNIITELAFFGGSFTALPADKQEQYLKIVQPYITNKTIRNIRLSTRPDA
                     ITDENLQLLNQYGVSIIELGVQSLDDEVLRLSGRGYTSAVVYEAAEKIIKHGFTLGMQ
                     MMIGLPGSTPEKDLLTAKEIIRCGAKGTRIYPLLVFRDTDLEQLYTNNEYTPLTVEEA
                     IEQSADLLELFLQAGVQVYKIGLHPSGFLDNGTLLAGPWIPAFRQHVQARVWQRKFEK
                     QMSSDHSIKGFVVHPSDINSAVGPNRCNANYFKEKGVSLLFRTDALINKGDFYAFYS"
     gene            complement(3820..5901)
                     /locus_tag="PKY63_11050"
     CDS             complement(3820..5901)
                     /locus_tag="PKY63_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01196.1"
                     /translation="MGLISGYPLWLIIVCIALGAVYAIVLYTGKKSKKWRPGIRYLLF
                     SLRFVVVTLLAFFLLEPLIRLNVKVSNDPVLIVASDNSKSMIASADSDFVKNKLPVMI
                     DDWLDGMENNLEIARYHFSDSLHAGPKADYNGNLTDIGNVFYEIRNRYINKNVAGIIL
                     ISDGIFNAGINPLAAAEQIQTPVFTVATGDTSLHTDAAITSLTGNRDVVFNNYFPVEF
                     SVSALQAAGQNIQVNVTHNEKNIFNSRLDVSGNQFSRVFNIRGFADKKGLNKIVVSIS
                     VLNGEKNITNNSRVFYFNVADEKKKVLIVASAPHPDIRAIRTSLESNLNYSVNAVTGE
                     ANAGDISDYDLLILHQIPTANTRNSPLIEKAKTARIPVWMITGSKTNYTSLNTLNAGI
                     NVRLRSTAANEVLGVINENFALFSIPQQETDFIKTMPPLTAAFGDYNVAPGTDVLMYQ
                     RIGNVITSQPLLCMSQVQGQRWAFLLGEGVWRWKLYDFQRNRSHELFDSFMSRIAQFL
                     CQNSDRSRFRVIYEPTHSETEPITFSAELFNQNFELINSEPIDLVLRDSSGKNFACSF
                     LPDGSGYSLNCGRLRPGEYSFTAQVKSKQYPYSQSGRITVDSYNPELANTQADFNLMK
                     LISHQTGGIMVSPENLNDISDFLKKNNLVQSTYYRENKYIDLLEWKWILFIILALVTT
                     EWVIRKREGYY"
     gene            complement(5908..6654)
                     /gene="fabG"
                     /locus_tag="PKY63_11055"
     CDS             complement(5908..6654)
                     /gene="fabG"
                     /locus_tag="PKY63_11055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPF01197.1"
                     /translation="MKLLEGKTAIITGAARGIGKAIAQTYAENGANVIITDLAVTEET
                     QQFVEELKKMGVKAKAFASNASVFADCEQLINDVTAEFPVIDILVNNAGITRDNLLMR
                     MTEQDWDLVLTVNLKSVFNMTKAIQKVMLKQRFGSIINMSSVVGIEGNAGQGNYAASK
                     AGIIGFTKSIAQELGSRNIHCNAIAPGFIETEMTAKLPDDVRNKWINDIPLKRIGTTK
                     DVANVAVFLGSELADYVSGQVISVCGAMQQ"
     gene            complement(7124..8800)
                     /locus_tag="PKY63_11060"
     CDS             complement(7124..8800)
                     /locus_tag="PKY63_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF01198.1"
                     /translation="MTNIIIPDSVMPFDFNGYMQMTIRNNFNLKALSDYDGDTWTFGQ
                     IGTEIAMLHEGFKAIGIKEGDKIALIGKNSANWCKIYLATITYGAVIVPILPDFRPAD
                     MHTIVNHSDSVLLFAEESVFKTLDLNEMPAIPMALRVRDFSVLNASDQTKADRFEQII
                     EQFKISHPDGIQPENVNYRHQTPDELIVISYTSGTTGFSKGVMLPQRSLLGNIEFARK
                     FMPMDPGERILSFLPLAHAFGCLVEFVFPFTLGCHITFLTKTPAPSTITEAFGKVKPN
                     LIPSVPLVIEKIYNGKIRPVISKPAMKILLAIPGIRRILLNKIKAKLTKTLGGEFREL
                     VIGGASFNPEAEKFFRQIRLPFTVGYGMTECGPLISYRGWRTTVFQGCGKIVEQLTLK
                     IASPDPENISGEIMVKGGNVMLGYYKNPEATKAMIDDDGWLHTGDIGTVDKNGNIFIR
                     GRMKNMILSASGQNIYPEEIEAVLANFRLFLEVLIVERKNKPVALIVPDADYMKKHGI
                     TEEQLPHKIKEIIKEANHHLASYMRVADFEIMKEEFEKTPKKSIKRFKYQ"
BASE COUNT         2398 a         1878 c         2024 g         2582 t
ORIGIN      
        1 aagcatatga aattgatccg gatcgccggg agatttatcc ggatttgatt gtgtattacg
       61 aaacgaacgg gaaatatgcg gacaaggaaa aagtattgat acatcaacag gaaattgcac
      121 cctcatcgcc tggaatgatg gcgtggaatt acaatgcgct ggcgacactc ggaaaaaacg
      181 caattgtgct gaccggtggc gacaacgata cttatcagaa atggactttg caggcggttt
      241 acaatatcag acccgatgta cgtgtgatca ataccagtct gatcatgata gaatcgtacc
      301 gcaacaggct ttttgcagaa acgggtattg caccttttac aacagccgtt gactcatcca
      361 attaccagac ttacacgcag ctgattgttg aacatatatg ccgcaacagt ggcacaaggc
      421 cggtatacat ctgtaattcg gttccggagg aacactatgc atcattgaag gatagtttat
      481 atctggaggg cattgtgtat aaatacagtc ctgaacggta cgacaatatt gcagtaatcc
      541 gaaggaacta tgaacagatc atgttgctgg attatattgt tgctccgatc tcggccgatg
      601 tttccagggc aatcgttgtc aattcaaatc tgaattatat ttctgcattc attcagctgt
      661 acgaccatta caggttgtgt ggtgaactgg ataaagctgt gcagctggca tccatccttg
      721 aaaagattgt gactgcatcg gacaacgctg agtatcggaa atatgtggaa gattatctga
      781 aagaaaataa cgatgctggc agagagtagg catgcggcaa ctttggttgg ttccgacaac
      841 gagctcctgg agcgggttgc cggagccgat catgctgcat ttaccgaact gtataaccga
      901 tgggcgggaa aaatccggta tttttttctt cgcttgtaca actatgatta tgatacagcc
      961 gatgatcgca cgcaggatac gtttctcaaa gtgcttgaaa atgcgcaaaa gtttgatcac
     1021 gatcaacgct tttctccctg gctgtatgcc attgcatgta atctgtacaa aaacgatgta
     1081 cgacgtcgga aacttgatca gcatttccgc gaagctgcta ttgctgaaac aacagtagaa
     1141 atgccgtctg cagaacgtaa ggtggaaatt cagtcttcag taaaactggt tcatcaatat
     1201 ctcgaaaaac tcgaccccga agtgaaatca atatttcttc tccgacatgc gggagagctc
     1261 actattcctg aaatcgcgcg tgtgactgga tgtccggaag gaaccgtaaa gtcgcgtttg
     1321 ttttatgcca tgaaaaaaat gtcggaaaac ctgcaaccac acacaataaa cgaatggata
     1381 tgaaaagtgc tgatttgttt gaggattttg aaaaactgct attaagcaaa gaatacgagc
     1441 agctgaatga agctgaaaga aatattattt tcatgtactg ctccggtccg caggaataca
     1501 gtgccatgcg tttattgatg cagcaaagcc aaacaatggc agcagagagc aataccatga
     1561 ctccgccatc cggtggcgca gaacaggttt gggcacgctc tgtcatgcaa aataacatca
     1621 acgaaaaacc gcatgggttc aatcgtacaa tatctgtgtg gtgggctgca ggactggccg
     1681 ctgcgctgat tctatcgtgg ctgatcaggt tcccggcttt cactcagaaa gaggttccaa
     1741 tgcttatccc ggatgttact actcgtttga tcactgaggt aaaaaccgac acggtgatca
     1801 aagaagttct ggtatatatt catcccgaaa cacaggctga cacttgtata cctgaatcgt
     1861 caaacgacgt tgtttcttat ggcggtatgg tgtatgtgca accggtcagt tcggtcgatg
     1921 agaacaaaat tcagcggcag ggaaccaatg cgagcgaaat gggcgatctg gctaaaatgc
     1981 aggtaaccgt gtactgatgc ttttttgaag cattgattat acaatcacaa ttccgcctgc
     2041 atcaacagcc tcagatttac caagaaaaat aatttccttg ttttgtcgat tggcgatttc
     2101 ggccagcagc cttgcatatg agctgccccg cgagccggtg aaaaacactt tgtcttcgga
     2161 ctgtccgagt gtgccgccga tgaatccgtt cgggactcct gaaagcgtga ttttgtcggg
     2221 ctttacaaaa tgaatgattt tgtttttttc ttttaactct ttttctattc cggcatcgga
     2281 tgttataaac tcgctcccta tgcgggccag attgcaggca atgtatcctt gttttacaga
     2341 aataatatct gcagatttta actgcgacag aattgattca tcggtatgtt tgctcacgac
     2401 tgcgatttca tcgtcgcaga aaacgttgta caatgcggtg tcgggataac ggaatccgac
     2461 ttttttattt ccctgaatga acgggatctg caattcattg aatagtgcag tgatgtcgga
     2521 aggcgcattg ggagacagaa tccattgatt gttgatatgc gccataaata tatccggatg
     2581 tccgcaaatg gccggataaa caatattgcc tgcatcgaaa agcgtgattt cgccgtatgt
     2641 ccggagattt tcaacgaggg ctgatgcccg gtaatcaaga ataaaatgca taaaaatctc
     2701 ctttgtttat gagcgcgtct gtccgaaaaa gtaaagatac tcctttttcc ttaaaataat
     2761 tggcattgca ccgattggga cccacagccg aattgatgtc ggacggatgc acaacaaatc
     2821 ctttgatgga atgatccgat gacatctgtt tttcgaattt ccgctgccac acgcgtgcct
     2881 gcacgtgttg ccggaaagcc ggaatccacg ggccggccag caatgtgccg ttgtcgagaa
     2941 atccggaagg atgcagtccg attttatata cctgaacgcc ggcttgcaga aagagttcca
     3001 gcaaatcggc cgattgttca atggcttctt cgacagttag tggcgtatat tcattgtttg
     3061 tgtaaagctg ctccagatct gtgtcgcgga agacgagcag aggataaatc cgggttcctt
     3121 ttgcaccaca gcggataatt tcttttgctg tcaaaagatc tttttcaggc gtgctgccgg
     3181 gcaatccgat catcatttgc ataccaagtg tgaagccatg tttaataatt ttctccgcag
     3241 cttcatatac aacagcagag gtatagccgc gtcccgaaag gcgcaatact tcatcgtcga
     3301 gcgactgaac acccagctca ataatactga ctccgtattg atttagcaat tgaagattct
     3361 catctgtaat ggcatcgggg cgtgtgctga ggcggatgtt gcggatggtt ttattggtga
     3421 tatatggttg aacaattttc agatattgct cttgtttatc tgctggcaaa gcggtaaaag
     3481 atccaccgaa aaacgccagc tcagtgatga tgttgcgctc ttttgtagtt gaaagatgtt
     3541 gttcaataat attttttatt tcatctgtat ccggcaccga atgctgtccg ctgatgtgtt
     3601 tttgattgca gaaaacgcat tgcttaggac agccaagttc cggtagaaaa acaggtatga
     3661 ttgagtgaga tttcattaat tatcggtgag cggtgattag tgagcggtga tttcagtctg
     3721 actgtgtccc agagcgattt tcctcattca gacttcactt ctttattctc acttccttgt
     3781 tcggcttgtt ccgtcagaag gcacggaatg ttcataattc tagtaatacc cttcacgttt
     3841 tctgataacc cattcagtag ttacaagtgc cagaataata aaaagaatcc acttccattc
     3901 aagcagatcg atgtatttgt tttcgcggta ataggttgat tgaaccagat tgtttttttt
     3961 gagaaaatcg gaaatgtcat taagattctc cggactcacc ataatgccac cggtttgatg
     4021 agaaatcagt ttcatcaggt tgaaatcggc ttgtgtattg gccagttcag ggttgtagct
     4081 gtcaactgtt attcgtccgc tttgtgagta tggatattgt ttgcttttca cctgagctgt
     4141 aaatgagtat tcgcctgggc gcagtcgtcc gcagttgagc gaataaccag agccatctgg
     4201 caggaaggag caggcaaagt tttttccgct tgagtcgcgt aaaaccaaat caatgggttc
     4261 actgttaatc agttcaaaat tctgattaaa aagttcagcg ctgaatgtga tgggttcagt
     4321 ttcggaatgt gttggctcgt aaatcactcg aaaccggctt cggtcgctgt tctgacaaag
     4381 gaactgagca atgcgcgaca taaaagaatc aaacaattcg tgcgatctgt ttctttgaaa
     4441 atcatacaat ttccagcgcc agactccttc tccaagcagg aatgcccatc gttgtccctg
     4501 aacctgactc atgcacagca gcggttgtga tgtaatgaca ttgccgatac gttggtacat
     4561 taaaacatca gtgcctggag caacattgta atcgccgaag gccgctgtca gcggaggcat
     4621 tgtttttatg aaatcagttt cttgttgtgg gattgagaat aatgcgaaat tttcattaat
     4681 aactccaagc acttcattgg cggctgtact acgcaatctg acattaattc cggcatttaa
     4741 tgtgtttaaa gaagtgtagt ttgtctttga tccggtaatc atccaaaccg gaatccgtgc
     4801 ggtctttgct ttttcaatca aaggactgtt gcgggtattt gctgttggaa tctgatgcag
     4861 aatcagtaaa tcataatctg aaatgtcgcc tgcattggct tcgcctgtaa cagcattgac
     4921 cgaatagttc agatttgatt caagtgatgt tcggatggcc ctgatgtcgg gatgtggagc
     4981 cgatgcaaca ataagcactt tcttcttttc gtccgcaaca ttaaagtaaa aaacacgtga
     5041 attgttggtg atatttttct caccatttaa aacagagatg gaaacaacaa ttttgttcaa
     5101 tcctttttta tcggcaaatc cgcggatatt gaaaaccctc gaaaactgat tgcctgaaac
     5161 atccagtctt gaattgaaaa tatttttttc attgtgcgtt acgttcacct gtatgttctg
     5221 tccggcggct tgcagagccg atactgagaa ttcaaccggg aagtaattat tgaaaacaac
     5281 atcacggttt ccggtaagcg aagtgatggc tgcatcggta tgtaaacttg tatctcctgt
     5341 tgcgactgtg aaaaccgggg tctggatttg ctctgcagcg gccagaggat taattccggc
     5401 attgaaaatc ccgtcgctga tgagtataat acctgccaca tttttattga tgtagcgatt
     5461 gcggatttcg taaaacacat taccaatatc agtcagattt ccgttgtaat cggctttagg
     5521 tccggcatgg agcgaatcgc tgaaatgata gcgggcgatt tcgagattgt tttccattcc
     5581 gtcaagccaa tcatcgatca tgactggtaa tttgtttttt acaaaatcag aatcagccga
     5641 agcaatcatc gattttgaat tgtcggaagc tacaatcagc acgggatcat tgcttacctt
     5701 cacattgagg cggatgagcg gttcaagcag aaaaaatgca agcagagtta caacgacaaa
     5761 acggagagaa aataagagat atctgattcc cggacgccat ttttttgatt tttttcctgt
     5821 atataacact atagcgtaaa ccgcacccag cgctatacac acaatgatta accacagcgg
     5881 atatccggaa atcagaccca taatgaatta ttgttgcata gcaccgcaaa cgctgatgac
     5941 ctgaccggaa acatagtcgg ccagttcaga gcccagaaat acagccacat tggccacatc
     6001 tttcgtggtg ccgattcttt tcagcggaat atcattgatc catttgttac ggacatcatc
     6061 cggcagtttg gcagtcattt ctgtttcgat gaaaccagga gcgatggcgt tgcagtgaat
     6121 attgcgtgaa ccgagttcct gcgctatact tttggtgaaa ccaatgatac cagctttcga
     6181 agcagcatag tttccctgtc cggcattgcc ttcgattcca acaacagagc tcatgttgat
     6241 gattgagcca aaacgctgtt tcagcatgac tttctgaatg gctttggtca tattgaaaac
     6301 tgatttcagg ttgacggtga gcacgagatc ccagtcctgc tctgtcattc gcatgagcag
     6361 attgtcgcgg gtgatgcctg cattgttcac tagaatgtca atgaccggga attcggcagt
     6421 gacatcgttg atcagctgtt cgcagtctgc aaacactgat gcgttgctgg caaaagcttt
     6481 tgctttgaca cccatttttt taagttcttc aacaaactgc tgtgtttctt cggtcacagc
     6541 caggtcggtt ataattacgt ttgcaccgtt ttcggcatag gtctgagcaa tggcttttcc
     6601 gattcctctt gcagcacctg tgatgatggc ggttttgcct tccagaagtt tcattttctt
     6661 tgtttttgaa tttgaggatg caaaagtaca attaaatgtg ctaatatgct aatgcgttga
     6721 agcacaacgc acaatgcaca atgcgcaacg atttgctagt ggaaagtcct taagtagaaa
     6781 agtgacttta atgcatgcgg catgcggtag cttcgctccc acacggctca ctcgctctgt
     6841 atttgacggg cggcacaaag ctcaaagcgc aacgcacaat gaataattaa gctgcgcact
     6901 cgctctttct catccacaga ggagccattc acatctgtta gcttttatct cgtatttttt
     6961 gcggcatcta atattcacac atcgcggtac ctgtcacagc ttgtcgcgtt ttacgtgatt
     7021 tcacgtgagg cgccacatcg gcgaagccgc acatgcgcga ctgtgtcacg ttttacgtga
     7081 agcgccacat atgtttgtat ttctgatctc gtatctcgta ttttcactga tatttaaacc
     7141 gcttaatgct tttcttcggt gttttttcga attcttcttt catgatttcg aagtcggcaa
     7201 cccgcatata ggatgccagg tgatgatttg cttctttgat aatctctttt attttgtgag
     7261 gtaattgctc ttcagtaatt ccatgttttt tcatgtaatc agcatccggg acaatgagcg
     7321 ctacaggctt gttttttctt tccacaataa gtacttcgag aaacaaacga aaatttgcaa
     7381 gtacggcttc aatctcttca ggataaatat tctgtccgga tgcgctcaga atcatgtttt
     7441 tcatgcggcc gcggataaaa atatttccgt ttttgtcaac tgtgcctata tcgcctgtgt
     7501 gcagccatcc gtcatcatcg atcatggctt tggttgcttc aggattttta taatatccca
     7561 gcattacatt gccacctttc accataattt caccgctgat attttcagga tccggcgagg
     7621 cgatctttag cgtaagttgc tccacaattt taccgcagcc ctgaaataca gttgttctcc
     7681 agccacgata tgaaatcagt ggaccacatt cagtcatgcc atagccaacc gtaaacggta
     7741 accgtatttg gcggaaaaat ttctctgcct ccggattgaa agatgctcct ccaatgacca
     7801 attcgcgaaa ttccccacct aatgttttgg taagttttgc tttgatttta ttgagtagaa
     7861 tgcgacgaat tccgggtatg gccagcaata ttttcatggc cggtttcgaa attacagggc
     7921 ggatttttcc attatagatt ttttcaatga ccagtggaac agaaggaatc aggttgggtt
     7981 tcacttttcc aaaggcctca gttattgttg atggagctgg agtttttgtc agaaaggtga
     8041 tatgacaccc cagcgtaaaa gggaaaacga attcgaccag acagccgaaa gcatgggcca
     8101 gaggcaggaa agacagaatg cgttcgccgg gatccattgg cataaattta cgtgcgaatt
     8161 cgatgttacc cagcaaactg cgttgcggca gcatcacacc tttagaaaaa cctgtcgttc
     8221 cggatgtata actgattaca attaattcat ccggtgtctg gtggcgataa tttacattct
     8281 caggctgaat accgtcagga tgcgatattt tgaattgttc tataatttgt tcaaagcgat
     8341 ctgcttttgt ctgatctgag gcattgagga cagagaagtc ccgaacgcga agagccatag
     8401 gaatggccgg catttcattc agatccagcg ttttaaagac agattcttcg gcaaacagca
     8461 aaaccgaatc gctgtggttc acaatggtgt gcatgtcggc aggtctgaaa tcgggcagaa
     8521 tgggtacgat gacagctcca taggtaatgg tcgccagata aattttacac cagtttgccg
     8581 aattttttcc aatcagggca attttatcac cttcttttat gccgatggcc ttaaagcctt
     8641 catgtagcat ggctatttca gttccgatct ggccaaaggt ccaggtatca ccatcgtaat
     8701 ccgaaagtgc tttcaggttg aaattatttc tgatggtcat ttgcatgtag ccgttgaaat
     8761 caaacggcat aacagaatca ggtataatta tgttagtcat atttggagga aataagattc
     8821 aaatgtataa atagggcctg ctgaaaaagt cacctttgtc agatttgaat aatctgagga
     8881 cg
//
LOCUS       DAOPKE010000108         8809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8418_length_8809_cov_11.443226, whole genome shotgun sequence.
ACCESSION   DAOPKE010000108 DAOPKE010000000
VERSION     DAOPKE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_8809_cov_11.443226"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..795
                     /locus_tag="PKY63_11065"
     CDS             205..795
                     /locus_tag="PKY63_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01199.1"
                     /translation="MKTTILFILFSLAGIVAFSQGSSLYAEITKTDITLTTNFSMETV
                     SVFGIHLGMTVEEVMATWAGNDDLYYYEDKNHGTKDYRIYVYDTDESGKKKNCILYLI
                     WTENRSELNRITVFNDFETYLQGNTKKLLSTEAVDSNSDFYKKYLGAPDSSAVTLDLP
                     SIGYKHTTFYYPHKFLQVTLKQDGDKASVVFAIYVL"
     gene            852..1271
                     /locus_tag="PKY63_11070"
     CDS             852..1271
                     /locus_tag="PKY63_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HPF01200.1"
                     /translation="MKVTSAQINEIAQMLEGGMFVYINPETFEMEFIPDLDDFGDSEL
                     WEELHNKVTEEWNSLVIEPMETWESFKIMENFIYELPDKKFQQNLIEILNRKRPFAHF
                     KAAIDNSDYRQQWFDFKEKMYEEYVKQQLEAEHIEFE"
     gene            1388..2047
                     /locus_tag="PKY63_11075"
     CDS             1388..2047
                     /locus_tag="PKY63_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPF01201.1"
                     /translation="MFRLYIKIILLRLSALLLPVALVQPVLSQNIRGEFISGFNLAQV
                     DGDEVVGYRKIGFNGGVGAALPLGRNWFVSVETLFNQKGAYKKFPPNNNNPGLPYYNL
                     RLNYAEIPLLIHYNDKDQVTAGLGVSYGRLVSMRETEHGVLIDWPTSTGPYSSEDFNI
                     LVDFRFRAFWKVHFNFRYAYSISKIRTRYYDNGASTWERDQFNNLLSFRLVFVFNEKL
                     K"
     gene            2220..4139
                     /locus_tag="PKY63_11080"
     CDS             2220..4139
                     /locus_tag="PKY63_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01202.1"
                     /translation="MRKVLLLGAALLVSAGIMAQSAAERTLVGASNKYKQVQKTYRIE
                     KAGQQTSVPVTSPAKNLNTKATQGITKTYMGTSANCFSVLLPEQAGLAANEETGIIMM
                     THRANGVDYNSGFILSSFSTDGGFSFDDSTVLIWENTGGYIARYPGGVIYNPTGNTTP
                     ANAYAVAAGPVLVSTWDGGFYASETFGGTNFAYDMTLHADTTGGAQPYNFLPRMHMQA
                     RGNKVFLYGDANTDDGTYYTSIHTTVNIGTFNTTTNSFDWVLSSHTPDYIIDGLGNPD
                     GNIYPGLVMDDAGSIGYLIYIGRNADASDNLTYQPIIYKTTDGGINWIEQPAFNWATC
                     PALQDFATDYSPVGRPLFSYVKDAVIDADGYLHFTNYVHGAYSDNVDSLGYYAVYTGM
                     QGIVFDTYQTSTGWASMAVDTIYASDPDEATTLIDPGTADYVAWDDRFQMSRTADGSK
                     IVYAWMDTDPSLSEVNIYPDIMVKMYDVATGTMGPKVNVTAGTDYDANNYWLYLADIT
                     FDKGTYYQVNLSTSTLNSNSSGTVDHEYVTGVYLDETGNLIAGVNDLPLDAAIAMYPN
                     PTSGDLNISFNDLAAGNYSVVVYNTIGGVVLSQNIDVNGAVVRSINMNELPTGIYMVQ
                     VSNENGSTTKKVIKN"
     gene            complement(4231..4467)
                     /locus_tag="PKY63_11085"
     CDS             complement(4231..4467)
                     /locus_tag="PKY63_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01203.1"
                     /translation="MVKIFDLESDQFIVDLTDEEFSALEEALEEDSIGDVDYYLHITD
                     VEFMEEEGAEKGLITKLKLMLEGREDREIRFQRI"
     gene            4542..5150
                     /locus_tag="PKY63_11090"
     CDS             4542..5150
                     /locus_tag="PKY63_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPF01204.1"
                     /translation="MKKNLPPFSVVIPAAGYSDRMGMPKLLLPFGHHTFCEEIFMRYH
                     LAGARKIVFVINQPLLSPCLKLFPNHTNKPDFIINKTPLMGRYKSIMIGLSCIPDNLP
                     CFLQNSDNPLVNESVLIKLLSESNPDYTAIPVFKNRNGHPVLIGTNIIKNLLKCTMPD
                     ANLRIELQKYNTKKIEVSTADILLNINTPEDYKEIFGRNISK"
     gene            5155..6468
                     /locus_tag="PKY63_11095"
     CDS             5155..6468
                     /locus_tag="PKY63_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01205.1"
                     /translation="MKTKTFIQYSMFIAILHLMACNSQECRFCNEKQQGAIYQNIRSE
                     GDTSVFYNIYVPAGTGHNKLPLLVLLDPHGNTAPALKQYTALADQYKVILAGFNASEN
                     EMPFGTIWNAFNPWIHELYKKAPIDSTLVFIAGFSGGARVATQIEGQMNNIIATALCG
                     AGPANISTWARKTAPVMMFTGTGDFNFMEINNLYSDFNIKRQRSLQLFAGTHQWPPAN
                     AFEDFFVLIKHLSDPDFVASEMYFLNNAKTHLKNQRPDMAFYSASNAAFAIGNENSEH
                     LRHFADSLKRNFPHDFSEKIIQCSKTETILQKRISSMFQNADTSEYFFLVDSINNQIA
                     TDSTTLNADMMRRTKAWCGIAAYSFASQAHRMNVPFLFSILRMYEYTEPGNPDMLILM
                     SAWYAKNKECMKARIYLNQAKRHGFNNQKKLDSMKEFDHCEDIRN"
     gene            6527..6859
                     /locus_tag="PKY63_11100"
     CDS             6527..6859
                     /locus_tag="PKY63_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF01206.1"
                     /translation="MAENDSSKLFYSIGEITERYKIRPSALHFWEKQFPELKPRRNKK
                     GNRFYTEEDIVLLDMIYYLTKIKGYTLKGARERIKADRKNIERNAAVSATLLRVKEFL
                     SELKDGLK"
     gene            complement(6961..7497)
                     /locus_tag="PKY63_11105"
     CDS             complement(6961..7497)
                     /locus_tag="PKY63_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01207.1"
                     /translation="MKKTLFLSAAFLLMSLLGIAQSTFKISSNGKPIVEIVVPTASWS
                     VTTTDSLFSIIPIDEGQTSRLITMIWASKNPHSETAIDDIANEAFDVVEALLEDITWN
                     EELSEFDNNGISFIAQDGQGYYANEDGSKDEMTTTVMLLMPDDVNVIALVYFGTPEAY
                     DKWSKSLLEIILSIIPAE"
     gene            7797..8618
                     /locus_tag="PKY63_11110"
     CDS             7797..8618
                     /locus_tag="PKY63_11110"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPF01208.1"
                     /translation="MKKVIIILFFGIISSTTFAQFDILFNFQNPTLLNPASVGSDSAM
                     NVFSVYQNNSNQFGNQYHSEKAGFTAPVPKLHGGFGLVASHEWSPSFNGSITNLTGIY
                     SYHKKTKSNLSFSFALMPELRINDFDDYSFEKRCQFNVSLAAGLRWKKFTFGASGYNI
                     INRNFPDNGFKLFAAYNQFQPGKLLDDKIHFSPMLSAFTFGGDVYLVASGTLRYKFMQ
                     VGADISSFIYLKSRPDFFFHAGVNIGNFSLLYRYCPDWYRTTINEISLGYRFGKK"
BASE COUNT         2707 a         1890 c         1665 g         2547 t
ORIGIN      
        1 ttgcgaagct agtcccgccg ggctacgaat ttggctctgc cccattttac gaatttgaca
       61 tttctgattt atcagaaatt caaatgagta aaaccagaat caaccttcca tctaacactt
      121 ttgatactat cgtgacttga tggacaatca aatgcaatca gctctcattt ccaaaaatat
      181 tctaattttg aaaaaacaat tcatatgaaa acaaccatcc tgttcatcct gttttcgctg
      241 gccggcatcg ttgcattcag ccagggaagc tcattgtatg ctgagattac caagaccgac
      301 ataacgctga caaccaactt cagcatggaa acggtcagtg ttttcggaat tcatctcggg
      361 atgactgtgg aagaagtcat ggctacttgg gcgggaaatg atgaccttta ttattatgag
      421 gataaaaatc acggcacaaa agattacagg atatacgttt atgacacaga tgaatccggt
      481 aaaaagaaaa actgcattct gtatctcatc tggaccgaaa accgcagcga gttaaaccgg
      541 attactgttt tcaatgattt tgaaacttac cttcaaggca ataccaaaaa acttctcagc
      601 acagaagccg ttgacagtaa ctcggatttt tataaaaaat atcttggggc tccggatagc
      661 tcagctgtga cactcgatct gccttcgatc ggttataagc atacaacttt ttattatcct
      721 cataaatttc tgcaggtcac actaaaacaa gacggagata aagcatcagt cgtgtttgca
      781 atctatgtcc tatagtgtac ctctaaaaac ctcattttgt atgaaatcaa tctttaacca
      841 attaaacacc catgaaagtc acttcagcac aaatcaacga aattgcccaa atgcttgagg
      901 gagggatgtt tgtttatatc aaccctgaaa catttgaaat ggaattcatt cccgatttag
      961 acgatttcgg agattcggag ctctgggaag agttgcacaa taaagtgaca gaagaatgga
     1021 acagccttgt gattgagccc atggaaacct gggaatcatt taagattatg gagaatttta
     1081 tttacgaatt acccgataaa aaatttcaac aaaacctgat tgaaattcta aaccgtaaaa
     1141 gaccattcgc tcatttcaaa gccgcaatcg ataactccga ctatcgtcag caatggtttg
     1201 attttaaaga aaaaatgtat gaggaatatg tcaagcaaca attggaagca gaacatattg
     1261 aatttgaata atcagtaatt gtttctttca caacaggaat ttgtactttt gacactgaaa
     1321 agataaaggc atacaataaa aaatgtgttt tttctttttt atcaaaattt caaatcactt
     1381 tcgctgaatg ttcagattat acattaaaat catattgctt cgtttgtcgg cattactcct
     1441 gccggtggca ctggtgcaac cagtcttaag ccaaaatatc cggggcgaat ttatttccgg
     1501 tttcaatctg gctcaggttg atggtgatga agtggttgga tatcgcaaga ttggcttcaa
     1561 tggcggagtt ggcgctgccc tgcctcttgg cagaaactgg tttgtgtcgg ttgagacctt
     1621 atttaatcaa aaaggagcat ataaaaaatt tcctccaaac aataacaatc cagggctgcc
     1681 atactacaat ctgcgactga attatgcaga gattccgcta ttgattcatt acaacgacaa
     1741 agatcaggtt acggccggat tgggcgtttc ctatggccgt ctggtatcga tgcgcgaaac
     1801 cgaacatggc gttctgattg attggccaac cagcacaggc ccttattctt cagaagattt
     1861 caacatcctc gttgatttcc gttttcgtgc tttctggaaa gttcatttta attttcgtta
     1921 cgcatattcc atatcaaaaa tcagaacacg atactatgat aatggcgcca gcacatggga
     1981 acgagatcaa ttcaacaatc ttctcagctt tcggctggta tttgttttca atgaaaaact
     2041 gaaataaagg cagctcagca ataaaacatc ttcttcggca aaagagtttg ttttctgaac
     2101 aaatttttaa atttatacga cagatgaggg ttttatgcaa ctccaatcat tttttttgtc
     2161 tttatataaa atatgttgta tatttgcagc caaatgtcaa accaaaatcc aaatcaatca
     2221 tgagaaaagt tctattgtta ggtgcagctt tgcttgtttc ggcaggcatc atggcacaaa
     2281 gcgccgcaga aaggacactt gttggagctt cgaataagta caaacaagtg cagaaaacgt
     2341 acagaattga aaaggccgga cagcagacct ctgttccagt aaccagcccg gcaaaaaacc
     2401 ttaacaccaa agccactcaa ggcattacca aaacgtacat gggcacctcg gcaaactgct
     2461 tcagcgtatt gcttcctgaa caggctggcc ttgctgcaaa cgaagaaact ggaataatta
     2521 tgatgaccca ccgtgcaaac ggcgttgatt acaactctgg tttcatcctc tcatccttct
     2581 caactgatgg tggtttctca tttgatgatt caaccgtttt gatctgggaa aataccggtg
     2641 gatatattgc ccgttatccg ggtggtgtta tttacaatcc aacaggaaat accactcctg
     2701 ccaatgctta tgcagttgct gccggtcctg tcctcgtaag cacctgggat ggtggtttct
     2761 atgcttctga aacatttggt ggaaccaatt ttgcctatga tatgacattg catgctgaca
     2821 ccactggtgg tgcacagcca tacaactttc tgccacgtat gcacatgcag gcacgtggaa
     2881 acaaagtatt cctctatggc gatgccaata ctgatgatgg tacatattat accagcattc
     2941 atacaactgt caatatcggt actttcaata ccacaaccaa cagtttcgac tgggtgcttt
     3001 caagccatac ccctgattac ataatagatg gtttgggcaa ccccgacgga aacatttatc
     3061 ctggtttggt tatggacgat gcaggaagca ttggatacct gatttatatc ggtcgtaatg
     3121 ccgacgccag cgacaatctt acttatcagc caatcattta caaaaccact gatggtggta
     3181 tcaactggat tgagcaacca gcattcaact gggctacctg cccggctctg caggattttg
     3241 ctactgatta ctctcctgtt ggtcgtcctt tattcagcta tgtaaaagat gctgttatcg
     3301 atgctgacgg ttatctgcat tttacaaact atgtacatgg tgcttacagc gataatgttg
     3361 actcacttgg atactatgct gtttacactg gtatgcaggg tattgttttc gatacctatc
     3421 agacatcaac cggatgggct tcaatggctg ttgataccat ctacgccagc gatcccgacg
     3481 aagctacaac cctgattgat ccgggtacag ctgactatgt tgcatgggac gatcgcttcc
     3541 agatgagccg cactgctgac ggatccaaaa ttgtttatgc atggatggat actgacccaa
     3601 gtctttctga agtaaacatt tatcctgaca tcatggtgaa aatgtatgac gttgccacag
     3661 gaaccatggg accaaaagta aacgtaacag ccggaaccga ctacgatgcc aacaactatt
     3721 ggctctatct tgccgacatc actttcgaca aaggaactta ctatcaggtc aatctgtcaa
     3781 catcgacact caactctaac agcagtggca ctgttgatca cgaatatgtt actggcgttt
     3841 atcttgatga aacaggaaac ctcatcgctg gtgtaaacga tcttcctttg gatgctgcta
     3901 ttgccatgta tcccaacccg acctcaggcg atctgaatat ctcattcaac gacctggctg
     3961 ccggaaatta cagtgttgtt gtttataaca ccattggtgg tgttgttctc agccagaaca
     4021 ttgatgtaaa cggtgcagtt gttcgttcaa tcaacatgaa cgaattacca accggaattt
     4081 acatggttca ggtttcaaac gaaaacggtt caacaaccaa aaaagttatc aagaactaat
     4141 cgttctgatc aatacaaaaa aagctgtccg cattgcgggc agcttttttt attcgaagtt
     4201 gtgctaagtt tgtgaacttc gcacaacttt ttaaattcgc tgaaaacgga tttcgcggtc
     4261 ttcgcgtcct tcaagcatca atttcagctt tgtaatcaat cctttttcag ctccttcttc
     4321 ttccataaat tcaacatctg ttatgtgcag ataataatcc acatcaccga ttgaatcttc
     4381 ttccaacgcc tcttcaaggg ccgaaaattc ttcgtcagtc aaatcaacga taaactgatc
     4441 ggactctaaa tcaaatattt ttaccatttt gtttgtgttt gaataacaaa cataaaaact
     4501 ttttcaatac cttttgcatt tcaatgtatt tttgtattga aatgaaaaaa aatctgcctc
     4561 ctttttctgt tgtaatacct gctgccggtt acagcgaccg gatgggaatg cccaaattgc
     4621 tccttccatt tggccatcat actttctgcg aagaaatttt tatgcgctat caccttgccg
     4681 gagcccgaaa aattgttttt gtcatcaatc agccattgtt gtctccatgt ttaaaactat
     4741 tcccgaatca cacaaacaaa cccgacttta ttataaataa aacgccatta atgggacgct
     4801 ataaatcaat aatgataggg ctttcgtgca tacccgataa cctgccgtgc tttttgcaga
     4861 attctgataa ccccttggtg aatgaatctg tcttgataaa actattaagc gaatcaaacc
     4921 ctgattacac tgcaataccc gtttttaaaa acagaaacgg ccatcctgtt ctgataggaa
     4981 ccaacattat taaaaatctt ctcaaatgca caatgccaga tgcaaatctt cgtattgaac
     5041 tgcaaaaata caacacgaaa aaaatagaag tttctactgc agatattctg ttgaatataa
     5101 acacacctga agactacaaa gagatttttg ggagaaatat ttcaaaatga ccatatgaaa
     5161 acaaagactt tcattcagta ttcaatgttt atcgccatac ttcatcttat ggcatgcaac
     5221 agtcaggaat gccggttctg caacgaaaag caacaaggcg ccatttatca gaatattcgt
     5281 tccgaaggcg atacttcggt tttctacaac atctatgtgc ctgcaggaac aggccacaat
     5341 aaactaccgc tgctggtatt gctcgaccca cacggtaaca cagctcccgc tttgaaacaa
     5401 tatacagcac tggccgacca atacaaggtt atccttgccg gtttcaacgc ctcggaaaat
     5461 gaaatgccat tcggaacaat atggaatgct ttcaacccgt ggattcatga gctttataag
     5521 aaagcgccca tcgacagcac actggttttc attgccggtt tttcgggagg cgcaagagta
     5581 gcaacacaga ttgaagggca aatgaacaac ataatagcaa cagccctttg cggtgcaggt
     5641 ccggccaaca taagcacctg ggctcgcaaa accgctccgg ttatgatgtt cacaggtacc
     5701 ggcgatttta atttcatgga aataaataat ctgtattcgg attttaatat caaacgtcaa
     5761 cgttcattgc aactttttgc gggcactcac caatggcctc cggccaatgc tttcgaggat
     5821 ttttttgtct tgatcaaaca tctgtccgat ccggattttg tcgccagtga aatgtatttt
     5881 ttaaacaatg ctaaaacaca tctcaaaaac cagcgccccg atatggcgtt ctattcggcc
     5941 tcaaatgcag cttttgccat aggtaatgaa aattctgaac atttgagaca ttttgctgat
     6001 agtcttaaac gaaattttcc acacgacttt tcggaaaaaa taatacaatg ctctaaaact
     6061 gaaacaatat tacaaaagcg catcagttct atgtttcaga atgctgacac ttcagaatac
     6121 ttttttctgg tcgattcaat aaacaatcag attgcaacag actccacaac tctcaacgcc
     6181 gacatgatgc gcagaacgaa ggcctggtgc ggaattgcag catattcatt tgcaagtcag
     6241 gcccacagaa tgaatgtccc gtttctgttc agcattctta gaatgtatga atacacagaa
     6301 cccggcaacc ccgacatgct cattctgatg tcggcctggt atgccaaaaa caaggaatgc
     6361 atgaaagcac gcatttatct caatcaggcc aagagacatg gattcaacaa tcagaagaaa
     6421 ctggattcta tgaaagaatt tgaccattgc gaagacatcc ggaattagac aattcacaaa
     6481 tgaattaggg tctgctgagg ggttattaaa atttgtattt ttgtgcatgg ccgaaaacga
     6541 tagcagcaag ttgttttatt ccatcgggga aattaccgaa cgatacaaga tacgtccgtc
     6601 ggcactgcat ttctgggaaa aacaatttcc ggaactcaaa ccccgcagaa ataaaaaagg
     6661 aaatcgcttt tataccgaag aagatattgt attacttgac atgatatatt atctcacaaa
     6721 aataaagggc tacacactca agggtgcacg tgaacgcatt aaagccgacc gcaaaaacat
     6781 tgaacgcaat gcagctgttt cagccacttt gctgcgggtg aaagagtttc tgtctgagtt
     6841 gaaagatgga ttgaaataag acgttaaaaa ttttgccagt tgcatcttaa aagtaaatcg
     6901 cacaaaatgc aactcggagc caggcatttt ttaataaaac agtcattacg gccaaacagt
     6961 ttattcagca ggaataatcg aaagaatgat ttccaacaag cttttactcc atttgtcata
     7021 ggcttcggga gttccaaaat agaccagtgc aatgacatta acatcatctg gcatgagaag
     7081 cataactgtt gttgtcattt cgtcttttga tccgtcttca tttgcatagt atccctgacc
     7141 atcctgtgca ataaatgata tgccattgtt gtcaaattct gacaattcct cattccatgt
     7201 gatgtcctcc agcagagctt caacaacatc aaaagcctca ttggcaatat catcaatagc
     7261 ggtttccgaa tgggggtttt tagaagccca aatcatggta atcaatctgg atgtttgacc
     7321 ctcatctatc ggaataatgc taaatagact atcagtggtt gtaacactcc atgaagcagt
     7381 tggaactaca atttcgacaa ttggttttcc attgctggaa atcttaaatg tgctttgggc
     7441 aatacccaat aacgacatga gtaggaatgc tgctgacagg aacaacgttt tcttcatggc
     7501 cgaaaggttt tgatgaataa ttctattgat ttgttgtgaa tcaaagataa tctaatttgc
     7561 attcaatcag aatatttatg tctgttctat tcaaaaaatc actgaaatga aaaattctga
     7621 aatgattttg ccagttataa ataaaggcac atatagccaa atttgtaatc aaatcaggtg
     7681 atcaattaca acttcaattc tacttttcac ggatcataag tctgaaccat ggaaaatatt
     7741 aacgaaacct tgtaacaatt cagagcgtat aaacgtttga gtaaaaacaa tgaactatga
     7801 aaaaagtcat tattattctt ttttttggaa taatcagttc cacaacattt gcccaattcg
     7861 atattctgtt taattttcag aatccgacac tactcaatcc ggcttcggtg ggaagtgata
     7921 gtgccatgaa tgtattttct gtctatcaaa ataactccaa tcaattcgga aatcaatatc
     7981 attcggaaaa agcaggtttt acagcgcccg ttccaaagct gcacggaggc tttggcttag
     8041 tggcatcaca cgaatggtct ccatcattca atggctcaat aacaaatctg accggcattt
     8101 actcatatca taaaaaaacg aaaagcaatt taagcttctc attcgcactc atgccagagt
     8161 taagaataaa tgatttcgac gattattcat ttgaaaaaag atgtcagttc aatgtttccc
     8221 tggccgccgg cctccgatgg aaaaaattca cttttggcgc aagtggatat aatatcatta
     8281 atagaaattt tccagacaat ggctttaagc tatttgccgc ttacaatcaa tttcagccag
     8341 gaaaactgct cgatgataaa attcatttct cacccatgtt gtctgccttt acatttggag
     8401 gagacgttta tctggtggct tcgggtacat tacgttataa gtttatgcag gtcggggccg
     8461 acatcagctc ttttatctat ttgaaatcaa gaccggattt tttctttcat gcaggcgtca
     8521 acataggaaa tttcagcttg ttgtaccgtt attgtcctga ctggtaccgg acgacaatca
     8581 acgagatttc gctgggctac cggtttggaa agaaataacg aagcctcaca aatccgtgac
     8641 agtaacttct ttcggcctca atttcctgta taccccgcct cgcttttcca gtcggagtag
     8701 tatatcacca gattagcttc acttttccat tcggtgaaat acactttcca gtttgcttcg
     8761 cttttccatt cagtaaaata ccagatgccg gaatcgggca tggcctcac
//
LOCUS       DAOPKE010000109         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8494_length_8758_cov_14.057681, whole genome shotgun sequence.
ACCESSION   DAOPKE010000109 DAOPKE010000000
VERSION     DAOPKE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8494_length_8758_cov_14.057681"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2243)
                     /locus_tag="PKY63_11115"
     CDS             complement(156..2243)
                     /locus_tag="PKY63_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01209.1"
                     /translation="MAKKTQTTSKFAPKAIPDGQSQLKLQTIFIVILGLALYSWTLTF
                     DYALDDTLMITGNKYTQQGFDGIPDIFSTDAFEGFFGGKGQVAGGRYRPFTHFIFAVE
                     VELFGFSPFVGHLFNVVTYIALLLVLFSFLRRLFPALSGTKEKFLSVPFVITALFAAH
                     PLHTEAVANIKGCDEIVTMLSAIGAMVLLYDYITKPKIWRLIAAFVLYFIALLSKENA
                     ITWIAVFPFILWIFSDAKKKDYFTTMSVLLIPAALFMFIRAQVVGGILETEIAPELLN
                     NPFIYSTKMQEIATVVFTWLIYFRLILFPHPLTHDYYPKQIAITDFSNPVVWITIAIV
                     IFSIIIFFRGLKKKSPVSIGILIFWATFSIASNLLFNIGTFMNERFMFVPLLGIAIIA
                     GCYFGKYYPRVKWLGIAFWILIGLYSAKTISRSLVWKNNKTLFLTDVKTSSKSAKVNV
                     SAAEVLLQEADAEKNMPKRQALANEALTYLNRAQKIHPVYFGVYDLRGKANYMVGQFK
                     ESLEDYKTCVSLDNSKKTIFNNIYLVGLGAIQNKNFTVAIDAFRFLVPYEPDSARNWL
                     QMAIVYDQLGMMNECFRCIDSALTVDSLYAPAWNKGGELFGRVRNDFATSEKYLLKSY
                     SIDPNNASVLENLGVLYGIMKQYQQSVHFLLKAHTKSPENKQILINLGTSYSGLGKKD
                     SAEYYLRMGGVMQ"
     gene            complement(2245..3528)
                     /locus_tag="PKY63_11120"
     CDS             complement(2245..3528)
                     /locus_tag="PKY63_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPF01210.1"
                     /translation="MKLLPVVEKYFNIHSFFLISSPMAKQWIFLFFVLHFFVVSAQQS
                     NNSFETVTDASVINGSERTEAYIHLLKNKQVAMVVNHTSMIDQRHIVDSLLASGINIS
                     TVFAPEHGFRGDMADGAHIHSYKDSLTGIDVVSLFGNSFKPSKKELENTDVVVFDIQD
                     VGVRFYTYISTMHYIMESCAENNIPLIILDRPNPNGHYIDGPMLDTDYRSFVGMHALP
                     IVHGLTIGELALMINGEKWLKDSMQCNLTVIPCLQWDHKKLYQLPIKPSPNLVSMESV
                     YLYPSLGLFEGTIISVGRGTMRAFEIIGHPLIRDTAFSFIPKPIAGMSEKPPLEGKIC
                     YGYDLKSFAGDYLKYRSEVFLTWIVELYCELDNDSHAGNFFNNKTFDRLAGGNKLRKQ
                     IANGLSVEEIKKSWQQDLEKYKQARKKYLLYEDFE"
     gene            3597..3815
                     /locus_tag="PKY63_11125"
     CDS             3597..3815
                     /locus_tag="PKY63_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HPF01211.1"
                     /translation="MKVYKIKITESLSRVVEIEAGSSEDAVAKVNGLYKNAVITLDSS
                     DYTEVNICELTDAELLEKLSGKNVKSLN"
     gene            3885..5108
                     /locus_tag="PKY63_11130"
     CDS             3885..5108
                     /locus_tag="PKY63_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF01212.1"
                     /translation="MNHSGFIARRLTAGKSKSVTGPVIRVSVFSIALSISVMLIAFAI
                     VRGFQHEVREKIIGFGAHIQIRPFDTNESLDQSPVQTGREDIDALHSIDGVKTVFPFV
                     EKGGLIKTDSNLHGVVMKGVNADFDFSFLQKNIVEGRLPDFSDTMSNDVLISQKVASM
                     LGLHTGSSLRVYFIIQDELQPRGRKFNVCGIFNTGMAEFDKIYSVCDMRHIQKLNRWG
                     DSLVSGYEILLNDFDDLPQVMTDVNNTIYYDLDASDIVTRQAQFFDWLALLDSNVFII
                     LVLMTVVAIINIISIILILILERIPFIGLFKALGAGNRMIRDVFVHVSVRLLLRGIII
                     GNVFGLGLILLQYFTHIFPLNEEMYYLGHIPVLLKADYIVGVNIGVFAISFLAVSLPA
                     LIVRRINPSAALQHK"
     gene            5121..6455
                     /locus_tag="PKY63_11135"
     CDS             5121..6455
                     /locus_tag="PKY63_11135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF01213.1"
                     /translation="MQLFVTIPAINELEFIPGVLADLAKQEYCDFTLLVCVNQPDAWW
                     HDESKLDVCNNNVATLNYVKSFCELHNIRLILQDKCSRGNGWTERQGGVGWARKTLMD
                     AVEAIADDDDVIVSLDADTRVETNYLAEIAKQFAAFQKASAMALPYFHRLQGDDDHKK
                     SMLRYEIYLRCYAINLLRIKHPFAYTPIGSAMAARVSAYRKIRGLTATSSGEDFYFMM
                     KLRKTGIIIPFCDTRVYPSSRKSHRVIFGTGPTVALGAPGQVERYPIYHPFLFNKIED
                     ACRAFGEIFITANLKAVERALMVFPDLEHEKLLRNHPQLIRFIHACYMKADAFAIYKF
                     VRSNQPADVSDMESVSALLKALQLPLDCLSFNEVSDTVHISTPLNVTECRDQDDNSGH
                     LHYNVLHNLSVEQLKSLRKLLMETEEKMLKKKYNDYFALHNDLKHPIWKYMS"
     gene            6437..7366
                     /gene="miaA"
                     /locus_tag="PKY63_11140"
     CDS             6437..7366
                     /gene="miaA"
                     /locus_tag="PKY63_11140"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPF01214.1"
                     /translation="MEIHELMVILGPTATGKTALAVKAARALNAEIVSADSRQIYRGL
                     DIGTGKDLSEYSQGGTAVPYHLIDFLNPGDSYNTFQFKADATEVIREIGSRNKLPLVC
                     GGTGLYIESLLLDYHFSSANRQEDFRREAEHMDNSVLARQIEVLGGTLSNEDKANRHR
                     LIRKLEVLLFSEEKKEMRESLKIEKLHVFGIDLPREIIRKRISDRLIFRLENGMVEEV
                     QALMDAGVSPKWLDALGLEYRFVSRYLKKQMSYQEMFSQLETAIHQFAKRQSTWFRRM
                     EKRGIKINWIDGQADDMEKMEFIMETVDFNCHG"
     gene            complement(7439..8248)
                     /locus_tag="PKY63_11145"
     CDS             complement(7439..8248)
                     /locus_tag="PKY63_11145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPF01215.1"
                     /translation="MRKFFNTIHTTIANIQTERENGSKASLRPFVVLFVSCCSLIVIE
                     YIGKSPGHYLLQQTSRWFGLNGFASFLEKHLEHSTNYQLYNLAYWAAIIFICYFIVPV
                     LVIKLGFRERLRDYGLQWGTFLKDSWIYLIMLGIMFPLVYFASTNHSFQARYPFYTPA
                     SGESLWPNFWIWQMIYFCQFFALEFFFRGFLVHGLKKYVGVYSIIIMTVPYCMIHFGK
                     PMGETFAAIFAGLALGMMSLKSRSIALGVFLHYSVAITMDMAALWQEGYFK"
     gene            complement(8333..>8758)
                     /locus_tag="PKY63_11150"
     CDS             complement(8333..>8758)
                     /locus_tag="PKY63_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPF01216.1"
                     /translation="AEIQQTSDFTYRLFDWGRTDKDGNARELHIENGLSALNYDDVST
                     GKTDPVRLGNTMDTLVNCSEFVTNRITLKAAQTVRREYITLDSFVILLCVEGNAVYEW
                     ENKEEPLRMGECILIPAIIDDLLISSKGGAEILEVFIQN"
BASE COUNT         2483 a         1739 c         1930 g         2606 t
ORIGIN      
        1 tgttttgtca ggcatcagcc aacaaaacgt tcaatgaaat tgctacaatt gactaaatta
       61 tattttcttt tggtattact tttttttgat ttcgatctcg accttgtttt tttgtgtatt
      121 ctgcaccgta agcctcatgc cgcatgcttt atgttttatt gcatcactcc ccccattctc
      181 agataatact ctgcgctgtc ttttttacca agcccgctat acgaggtgcc caggttgatc
      241 agaatttgtt tgttttccgg actttttgtg tgggccttga gcaaaaagtg tactgattgt
      301 tgatactgtt tcatgatacc ataaagtacc ccaagatttt caagtactga tgcattgttg
      361 gggtcaatgg aatatgactt gagtaaatat ttttcagagg ttgcaaaatc attccggact
      421 ctgccaaata gctctccgcc tttgttccac gccggtgcat acaaactgtc cactgttaat
      481 gccgaatcga tgcaacggaa acactcattc atcataccga gttgatcata tacaatggcc
      541 atctggagcc agtttcgggc cgagtcgggt tcatagggaa caaggaaacg gaaagcatcg
      601 atggccacag tgaaattttt gttttgaatg gctccaagac caaccagata aatgttgttg
      661 aaaatggttt tttttgaatt atcaagacta acacatgttt tgtaatcttc aagtgattct
      721 ttgaattggc ctaccatgta atttgctttt cctctgagat catacacccc aaaatagacc
      781 ggatgaattt tctgtgcccg gttcaggtat gtaagagctt cgtttgccaa tgcctgccgt
      841 ttgggcatgt ttttttcagc atcagcttcc tgcaaaagaa cctcggctgc agaaacattg
      901 acttttgcac ttttcgacga cgtttttaca tcggttagaa atagtgtttt gttgtttttc
      961 cacaccagac tgcgcgaaat tgttttcgcg gaatacaggc cgatgagtat ccaaaatgcg
     1021 attccgagcc atttcacacg tggataatac ttgccgaaat aacaccctgc aatgatggct
     1081 atccccagca gcggaacaaa cataaaacgt tcgttcatga atgtgccaat gttgaaaagc
     1141 agattggatg cgatggagaa cgttgcccag aaaatcagta ttccaatgga aacaggtgat
     1201 ttctttttta atccgcggaa gaaaatgatg atggaaaata tgactatggc gatggttatc
     1261 catacaaccg gattgctgaa atcggtgatg gctatttgct ttggataata atcgtgcgta
     1321 agcggatgtg gaaacagaat caatctgaaa tatatcagcc aggtgaaaac aaccgttgca
     1381 atctcctgca tttttgtaga ataaataaaa ggattattca gtaattccgg agctatttct
     1441 gtttcaagga ttccaccaac cacctgtgcc cgaatgaaca tgaatagcgc tgcaggaata
     1501 agaagcacgc tcattgtagt aaagtaatct ttctttttcg catcgctgaa aatccacaat
     1561 ataaaaggga acacggcaat ccatgtgatg gcattttctt ttgaaaggag tgcaataaaa
     1621 taaaggacaa atgctgcaat cagtcgccag atcttcggtt tggtaatgta gtcgtacagt
     1681 aataccatgg ctcctattgc actgagcatg gtgacaatct catcgcatcc ctttatgttt
     1741 gctacggctt cggtgtgcag cggatgcgcg gcaaacaagg ccgttatgac aaatggcact
     1801 gataaaaact tttcttttgt tccggacaga gcggggaaca ggcgtcgcag aaaagaaaac
     1861 aaaacaagaa gcaaggcaat gtatgtaaca acattgaaga ggtgaccaac gaaaggacta
     1921 aagccaaaca attcaacttc tacagcaaaa ataaaatgcg tgaatgggcg gtagcgtcct
     1981 cctgctacct gtcctttgcc tccgaaaaat ccttcgaaag catcggtgga aaagatgtca
     2041 ggtattccat caaaaccttg ctgcgtgtac ttgttgccgg tgatcatcaa tgtatcatcc
     2101 agcgcgtaat cgaacgtgag cgtccaactg tacagtgcca gccccagaat gacaatgaaa
     2161 attgtttgca acttcaactg gctttggcca tcaggaatgg cttttggtgc aaatttcgat
     2221 gttgtctgtg ttttctttgc cattttattc aaagtcttcg tacaacaaat attttttacg
     2281 tgcctgtttg tatttttcaa gatcctgttg ccaggatttt tttatttctt cgacagataa
     2341 tccgtttgca atctgcttcc ggagcttgtt tccgccagcc agccggtcaa aggttttgtt
     2401 gttgaaaaaa tttcctgcat gcgaatcatt gtcaagttcg caatacaact ccacaatcca
     2461 ggttagaaaa acttcgctgc ggtattttaa gtagtctccc gcaaacgatt tcaggtcata
     2521 gccatagcag attttgcctt caagtggagg tttttccgac atgcctgcta tcggttttgg
     2581 aataaatgaa aatgcggtgt ctctgatcag cggatgtccg ataatttcaa atgccctcat
     2641 ggttccacga ccaacactga tgatggttcc ctcaaataat ccaagcgaag gatataaata
     2701 cactgattcc atggaaacaa gattgggact gggttttatg gggagctgat ataatttttt
     2761 atgatcccat tgcaagcagg gaataacggt cagattgcat tgcatgctgt ctttaagcca
     2821 tttttctcca ttgatcatca acgccagttc tccaatggta agaccgtgaa caatgggtag
     2881 tgcgtgcatg cccacaaagc ttctgtagtc tgtatcaagc attggcccat cgatatagtg
     2941 cccatttgga ttggggcggt caaggataat gagcggaata ttgttttcgg cacacgattc
     3001 catgatgtaa tgcatggttg aaatgtatgt gtaaaaacgc accccgacat cctgaatgtc
     3061 gaaaacaacg acatccgtgt tttcaagttc tttttttgag ggtttgaatg aatttccgaa
     3121 aagagataca acatcaattc cggtaagact atccttgtag ctgtgaatat gagctccgtc
     3181 agccatatcg cctcggaagc catgttcagg agcgaaaaca gtgcttatat taattcctga
     3241 tgccagcagt gaatcaacga tgtggcgttg atcaatcatc gaagtatggt taacaaccat
     3301 ggcaacctgt ttgttcttaa gcaggtgaat ataggcctcg gtgcgctcgg aaccgtttat
     3361 tacgcttgca tctgttacag tctcaaatga attattactt tgttgtgcac tgacgacaaa
     3421 gaaatgcaat acaaaaaaca gaaaaatcca ttgtttagcc ataggagacg aaattagaaa
     3481 aaaagaatga atattgaaat atttttcaac aaccggcaga agtttcaatt aaaattctat
     3541 ttttgcgacg ttgctgttta aaaacttgtt ttgtaatcaa tgataaatta cctaaaatga
     3601 aagtatataa aattaaaatt actgaatctc tttcccgtgt tgtggaaatt gaagccggct
     3661 cgtcggaaga tgcggttgca aaagtgaatg gtttatataa aaatgccgtt ataacgcttg
     3721 attcatctga ttatacagag gtgaatattt gcgagctgac tgatgctgaa ttattggaaa
     3781 agctgtcggg gaaaaatgtg aaatcgctga attaatccct accctgacat tttttacgat
     3841 gcatttttgt gcgattttta ttacatttgc acaaacagaa ctgcttgaat cattcaggtt
     3901 ttatagcccg tcgtcttaca gccggaaaat cgaaaagtgt taccggtcct gttattcgtg
     3961 tatccgtttt tagtatcgcg ctgagtattt cggtaatgct gattgctttt gccatcgtgc
     4021 gcggatttca acacgaagta cgtgagaaaa ttattggttt tggcgctcat atacagatac
     4081 ggccgtttga caccaacgaa tcactggacc aatctccggt gcaaacaggt cgcgaagata
     4141 ttgatgcact gcattccatt gatggggtga aaactgtttt tccgtttgtt gaaaaaggtg
     4201 gattgataaa aacagacagc aacctgcatg gtgttgttat gaaaggcgtg aatgctgatt
     4261 ttgatttctc ttttttgcag aaaaatatag tcgaaggtcg ccttccggat ttttcagata
     4321 ccatgtcgaa tgacgtgctt atttctcaaa aagtggcttc tatgcttggt ttgcatacgg
     4381 gaagctcttt gcgtgtgtat tttatcattc aggatgagtt gcaaccgaga ggaaggaaat
     4441 tcaatgtgtg cggaatattt aatacaggga tggctgagtt tgataagatt tattcggtat
     4501 gtgatatgcg gcatattcag aaattgaaca gatggggcga ttcgctggtg agcggctatg
     4561 aaatactgct caatgatttc gacgaccttc cgcaggtaat gaccgatgtg aataatacaa
     4621 tttattatga tctcgatgct tcggatattg ttacaaggca ggcgcagttt ttcgattggc
     4681 ttgcattgct cgattccaac gtgtttatca ttctggttct gatgactgtt gtagccatta
     4741 tcaatattat ttcaatcatc ctgattctga ttctcgagcg gattcctttc attggcttgt
     4801 ttaaagcact gggagccgga aaccgtatga tacgcgatgt gtttgtgcat gtttctgttc
     4861 gacttctttt gcgtggaatt attatcggaa atgtgtttgg attggggctg atattattgc
     4921 aatatttcac tcatattttt ccgcttaatg aagaaatgta ttatctcgga catattcctg
     4981 ttttgctgaa ggccgattat attgttggtg tcaatatcgg cgtgtttgct atttcttttt
     5041 tggcagtttc ccttccggct ttgattgttc gccgcatcaa tccttcagct gctttgcagc
     5101 ataaataaag atcggaaatc atgcagcttt ttgtaaccat tcctgccatc aatgaactgg
     5161 agtttattcc cggagttctt gctgatcttg caaagcagga gtattgtgat tttacattgc
     5221 tggtttgtgt caatcagccc gatgcatggt ggcacgatga atcaaaactg gatgtatgca
     5281 acaacaatgt ggcaaccctg aactatgtaa aatcgttttg tgaattgcat aatattcgtc
     5341 tgattcttca ggataaatgt tcgcgtggaa acggttggac tgaacggcag ggaggtgtgg
     5401 gttgggctcg caaaacactg atggatgctg tggaagcaat cgccgatgat gatgacgtga
     5461 ttgtttcgct tgatgctgat acacgcgtcg aaacaaatta tctggcggaa attgcaaaac
     5521 aatttgcagc ttttcaaaaa gcttcggcaa tggctttgcc ttattttcac cggcttcagg
     5581 gcgatgatga tcataaaaaa tcaatgctgc ggtatgaaat ttatctgcgt tgttatgcaa
     5641 taaatctgct tcgcattaaa catccttttg cttatactcc catcggaagc gcaatggcag
     5701 cgcgcgtttc ggcataccga aaaatacgcg ggcttacagc aacatcttct ggtgaagatt
     5761 tttatttcat gatgaaactt cgcaaaaccg gcatcattat tccgttttgc gatacacgtg
     5821 tttatccttc gtcgagaaaa tcacaccggg tgatttttgg caccggccct acagttgctc
     5881 tgggcgcgcc cgggcaggtg gagcgatatc cgatatacca tccgttttta tttaataaaa
     5941 ttgaagatgc ctgtcgtgcg ttcggtgaaa tctttattac agcaaatctc aaagccgttg
     6001 aacgcgcgct gatggttttt cctgacctcg aacatgaaaa gcttctgaga aatcatcctc
     6061 agcttattcg ctttattcat gcctgctaca tgaaggccga tgcgtttgct atatacaaat
     6121 ttgtacgttc gaatcagcca gccgatgtga gtgatatgga gagtgtttca gcgttgttga
     6181 aagcacttca gttgccattg gattgtctat ccttcaatga ggtttctgat actgttcaca
     6241 tttcgactcc gctcaatgtg acagaatgca gggatcagga tgacaattca ggccatctgc
     6301 attataatgt attacataat ctgagcgttg aacaattgaa atctctgcgt aaacttctga
     6361 tggaaacaga ggaaaaaatg ctgaagaaaa aatataatga ctactttgca ttgcataacg
     6421 acttaaaaca ccccatatgg aaatacatga gctgatggtt attcttgggc ctactgcaac
     6481 gggaaagact gcgttggccg taaaagctgc gcgtgcgttg aacgcagaaa ttgttagtgc
     6541 cgatagccgt cagatttatc gcggccttga tatcggaact ggcaaagatt tgtcggaata
     6601 cagccaaggc ggtactgcag ttccatatca tctgatcgat tttctcaatc ccggagattc
     6661 atacaatact tttcagttta aagccgatgc aaccgaagta atcagagaaa tcggaagccg
     6721 caacaaattg cctttggtat gtggagggac tggtttgtat attgaatcgt tgttgcttga
     6781 ctatcatttc agttcagcca atcgtcagga agatttccgc cgcgaagctg aacatatgga
     6841 caatagcgtt ttggctcgac agatcgaagt tttgggcgga actctttcaa atgaagataa
     6901 agccaatcgt catcgactga tacgaaagct tgaagtgttg ctgttttcgg aagagaaaaa
     6961 agaaatgcgc gagtcgttga aaattgaaaa gctgcatgtt tttggaattg atttgccgcg
     7021 cgaaattatc cgtaagcgaa tttcggatcg tctgattttt cgtcttgaaa acggcatggt
     7081 cgaagaagta caagcattaa tggatgctgg tgtatcgccg aaatggctcg atgcacttgg
     7141 ccttgaatac cgctttgtga gccgttatct gaaaaagcaa atgagctatc aggaaatgtt
     7201 ttctcaactt gaaacagcca tacatcagtt tgcaaaacga caaagcactt ggtttcgccg
     7261 tatggaaaaa cgagggatta aaatcaactg gatcgatggt caggccgatg atatggaaaa
     7321 gatggaattc atcatggaga ctgtggattt taactgccac ggctaatttt aaaatgcccg
     7381 atattagttg ccagttgtca gtggtaaaca gtcagtgaaa cttttgactc cccactcatt
     7441 acttaaaata cccttcctgc cacaaagcag ccatgtccat ggtaatggca acgctgtaat
     7501 gcagaaaaac accgagcgcg atgctgcggc tttttaagct catcatgccg agggcaagtc
     7561 ctgcaaaaat ggctgcaaat gtttctccca tgggctttcc gaaatgaatc atgcagtaag
     7621 gaacggtcat gattattatt gaatatacgc ccacatattt ttttaatccg tgtaccaaaa
     7681 aaccacgaaa gaaaaattca agcgcaaaaa actgacagaa ataaatcatt tgccagatcc
     7741 agaaattcgg ccacaaggat tcgccggaag ctggtgtgta gaaaggatag cgggcctgaa
     7801 agctgtggtt ggtactggca aaataaacca gcggaaacat aatgcccagc atgatcagat
     7861 aaatccacga atctttcaga aatgttcccc attgcaatcc gtaatcgcgc agtcgctcac
     7921 ggaaacctaa tttaataacc agtaccggca cgatgaaata gcagatgaag ataatggctg
     7981 cccaatacgc gagattatac aactgataat ttgtggaatg ttcaagatgt ttttccagaa
     8041 atgatgcaaa tccgttcaat ccaaaccatc gcgaagtctg ctgcagcaga taatgtccgg
     8101 gacttttgcc gatgtattca atcacaatca gtgaacaaca ggatacaaac aaaacaacga
     8161 agggtcgaag gcttgccttg cttccatttt cgcgctctgt ctggatgttg gcgatggtgg
     8221 tgtgtatggt attgaaaaat tttcgcacgc agttttacat tgaaagattg caaaaataca
     8281 atttatgcat tatcaatgct tgctttggcg aaaaatagtt atggagataa aactaattct
     8341 gaataaacac ttccagaatt tcagcgccgc ctttggatga aatcagcaga tcatcaatta
     8401 ttgcgggaat cagtatgcat tcgcccatgc gaaggggttc ttctttgttt tcccattcat
     8461 aaacagcatt gccttctacg cagagtaaaa tgacaaacga atcgagggta atatattcgc
     8521 ggcgcactgt ctgtgcggct ttcagtgtaa tgcgattggt aacaaattct gaacaattta
     8581 ctaaggtgtc cattgtattt cccaatcgaa ccgggtcggt ttttccggta gagacatcgt
     8641 cgtaattcag cgcactgaga ccattttcga tatgcaactc gcgagcattg ccatctttgt
     8701 cggtgcgacc ccaatcaaac agccgatatg taaagtcgct tgtctgctgg atttcagc
//
LOCUS       DAOPKE010000110         8705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_8561_length_8705_cov_11.810405, whole genome shotgun sequence.
ACCESSION   DAOPKE010000110 DAOPKE010000000
VERSION     DAOPKE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8561_length_8705_cov_11.810405"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(479..1246)
                     /locus_tag="PKY63_11155"
     CDS             complement(479..1246)
                     /locus_tag="PKY63_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPF01217.1"
                     /translation="MFFILSKILAFLFSPITWILALIALSLFLRKPKLKKRFLIAALA
                     TGLFFTNSFIAKEFMRMWEPEPLSVYSIQKKYDAVIVLGGGIVTTNAQNKTLTFRNNP
                     DRIMQALWLYKNDKADKILISGGSGSMQYPDMLESVLLRDYLIDIGIPAEDILIDSLS
                     NNTRENAVNTAKILNDSIPGGEFLLMTSASHMRRSIGCFRNVNLKVDPFPVDLTAGDR
                     SWYWMNLLLPKTDALDIWDELLHEIIGYLTYRIVGYI"
     gene            complement(1254..2045)
                     /locus_tag="PKY63_11160"
     CDS             complement(1254..2045)
                     /locus_tag="PKY63_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPF01218.1"
                     /translation="MNNQIDSKIADWLNEKADFYNNPSFVETDPVSIPHLFSKKEDIE
                     ISALFSALLAWGNRKAIISGAKQLMQRMDNVPFQFVTGHTQKDLKKLQGFVYRTFNSF
                     DAITLCEGLKSIYLHEGGMQSVFSKPILRNKNSNAFDAIVHFRNILLSYPHEVRFEKH
                     ISSPERGSAAKRLNMFLRWMVRRDDRGVDFGLWTDTFPMSRLLIPLDVHSARVASETG
                     MLSRLQTDAKAVIEVTENCRLLDPDDPARFDFALFGTGINEKHYF"
     gene            complement(2197..2571)
                     /locus_tag="PKY63_11165"
     CDS             complement(2197..2571)
                     /locus_tag="PKY63_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPF01219.1"
                     /translation="MKEIIYKDESFSIVGAAMEVHKTLGNGFLEAVYHEALMIEFEKR
                     GIPYKHEEPLFIKYKEVQLKKSYVADFVCYEKIIVELKALSQLVSDHESVVLNYLKAS
                     GFKLGMLINFGASSLEHKRLVK"
     gene            complement(2989..3540)
                     /locus_tag="PKY63_11170"
     CDS             complement(2989..3540)
                     /locus_tag="PKY63_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPF01220.1"
                     /translation="MTEEMIIAGFGGQGVLSMGKILAYSGMMQGLEVSWMPSYGPEMR
                     GGTANVTVIISDNRISSPILNQFDTAIILNQQSLDKFESSVKPGGVLIFDPNGIVNPP
                     TRKDVQIYEILATKKASDLGLSKIFNMIVLGGFLKVKPVVTDQFIEAGLKKSLPTRHH
                     NLIPDNVKAVGIGREIIVPYTVK"
     gene            complement(3558..4358)
                     /locus_tag="PKY63_11175"
     CDS             complement(3558..4358)
                     /locus_tag="PKY63_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF01221.1"
                     /translation="MADVTLDVWKDIIKPENKVFSKTPLLTDKVFSYCPGCGHGTAHR
                     VVAEVIEEMGIQKDTIGIAPVGCSVLAYEFLNIDMQQAAHGRAPALATALKRMWPEKY
                     IFTYQGDGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLPGMKSSTS
                     PYGREVEMMGNPLRMTELVAQLPGTYFVARHAVHKPGNVRKFKKAVRQAFENQKNNKG
                     LSFIEVVSNCNSGWKMTPVAANEWMEQNMFPFYPLGDIKVDGQMVTKK"
     gene            complement(4363..5442)
                     /locus_tag="PKY63_11180"
     CDS             complement(4363..5442)
                     /locus_tag="PKY63_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPF01222.1"
                     /translation="MGELKLMKGNEAVAEAAIRAGCDGYFGYPITPQSEVIEYLMAEK
                     PYERTGMVVLQAESEVASINMLYGGAGSGKRVMTSSSSPGISLMAEGISYMAGAELPC
                     LLVNVVRGGPGLGTIQPSQCDYFQTVKGGGHGDYRMFVLAPASVQEMSDFVKIGFEMA
                     FKYRTPAMLLSDGVIGQMMEKVELFDPIPRRSEAEIIKECPWATTGKTPDRERNIITS
                     LDLDSAKQEEHVIKLGKKYESMKQDVMFENIMTDDAEYLFVAYGSSARICQKAVDLAR
                     AEGIKVGLHRPITLYPFPEAELNRLASKVKGMLAVEMSLGQLVEDVRLAVNGKIKVDH
                     YGRIGGMIHTPTQVVDALKKQIIGG"
     gene            complement(5460..5684)
                     /locus_tag="PKY63_11185"
     CDS             complement(5460..5684)
                     /locus_tag="PKY63_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF01223.1"
                     /translation="MAKVKGAIVIDRDRCKGCGVCVPVCPNSVIRLGDKVNIKGYFYA
                     EPYQDACIGCMNCATVCPDAVITVYSKKVE"
     gene            complement(6033..7310)
                     /gene="metK"
                     /locus_tag="PKY63_11190"
     CDS             complement(6033..7310)
                     /gene="metK"
                     /locus_tag="PKY63_11190"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPF01224.1"
                     /translation="MGILFTSESVSEGHPDKVADQISDALLDEFLRHDPKSRVACETL
                     VTTGLVVLAGEVTTTGWIDQQETARHIIQKIGYTKPEYSFDSNSCGIISSIHGQSPDI
                     NQGVDREKEEEQGAGDQGMMFGYACDEMDNYMPMPVELAHIFVQKLADIRKEGKLMTY
                     LRPDSKSQITIEYDDNRVAQRIDTIVISTQHDENVSLDQIRSDVQSILIPRVIESKDT
                     PERVKNLFKNDFTLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGRGAHGGGAFSGKD
                     PSKVDRSAAYAARHIAKNLVAAGVAKEVLVQVAYAIGVAQPVGFNVNTFGTAKVKMTD
                     AEISNIVRGLFDLRPYAILKEYDLCNPIYTPTAAYGHFGRSTYEQEVEVSELHTGAVK
                     KQTPQGEKWMKKITFFAWEKLNRVDDIKKAFGL"
     gene            complement(7839..8567)
                     /locus_tag="PKY63_11195"
     CDS             complement(7839..8567)
                     /locus_tag="PKY63_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01225.1"
                     /translation="MKILLSIICSLFFVAYINAQGKSEGTQCYDMQTGWFAGVAGGQL
                     LPYTDVTSYDGFHFTEQYNEVSGGYAIRFGKHWAPAFSSNLEVFDGRLDGTKMFYTND
                     YPTDQAFTCNFWTLSADFQAHLFKLTNITNRSCKFLPFDIYGRLGAGATFYRAQQRIA
                     TTGVVVSEVGMDKREMVFTYIFGFGSEINILPMLSIYGEYSWYNTPTDLLDAREGITN
                     SDDAFGSFMGGIKFYFGGKNCSIF"
BASE COUNT         2520 a         1885 c         1817 g         2483 t
ORIGIN      
        1 aagctcatgt tggatatatt atagatggat ttccatataa aaacttgtag aacctttttt
       61 aataatggaa gctatccgca gatgcaaagc aatccagcat tccatggtta gaagctctgc
      121 aaagcaacat gagactaaat gaaaaaaata aaatgtcgat cggaacaaat tcattatgat
      181 tttgttgctt gatgccagag aggttgccta ttgaaatact ttgtcagaat atacaactct
      241 ttatatgcga ctcctccgga gtcgtcgctt tgtgcattta tggactacag atatgtgact
      301 cctccggagt cttccaaaaa tgattgcaac aatgcagcaa ttgctttggt ataaaatttc
      361 cggagaacat ggaatagttg atgcgaggtc tcagtatccg actcttccag gtttttcatt
      421 gctttacaaa ctctggaagg tcgggagcaa cagttttttt acggggcttc aaatgttttt
      481 aaatataccc aacaatccgg taggtcagat atccgataat ttcgtgcagc agttcatccc
      541 atatatcgag cgcgtcggtt ttgggaagca gcagattcat ccaataccag ctgcggtcgc
      601 cggcggtaag atcgaccgga aaaggatcga ctttcagatt gacattgcga aagcaaccaa
      661 tgctgcgccg catgtgcgat gcagatgtca tcaataaaaa ctcaccgccg gggatggagt
      721 cgtttaaaat cttcgcagta ttgacagcgt tttcgcgggt attgtttgac aaactgtcaa
      781 tcagaatatc ttcagcggga ataccgatat caatcagata atcgcgcagt aaaacacttt
      841 caagcatatc gggatattgc atggaaccgg agccgcccga aattaaaatt ttatcggcct
      901 tgtcgttttt atacagccat aatgcctgca taatgcggtc gggattattg cggaatgtta
      961 atgttttgtt ttgcgcattg gtagtaacaa tgccacctcc aagcacaatg accgcatcgt
     1021 attttttttg gatggaatat accgacaacg gctccggttc ccacatgcgc ataaactcct
     1081 tggcaataaa agaattggta aaaaacaaac ccgttgccag tgcagcaatg agaaatcgct
     1141 ttttcagttt tggtttgcgc agaaacagcg aaagcgcaat cagtgccagt atccaggtga
     1201 ttggactgaa cagaaaagct aatattttgc tcagaataaa aaacatattg ctgtcaaaaa
     1261 tagtgttttt cgttgatacc agttccgaac aatgcaaaat caaaacgggc ggggtcatct
     1321 ggatcaagca gccggcagtt ttcggtcact tcaatcacag ctttggcatc ggtctgaagc
     1381 cggctcagca tgccggtttc gctcgctacg cgggctgaat gtacatcgag aggaatgagc
     1441 aatcgactca ttggaaaagt atcggtccat aatccaaaat ccacccctct gtcgtcgcgc
     1501 ctgaccatcc agcgcaggaa catattgagc cgcttggccg cgctgccccg ctccggcgaa
     1561 ctgatgtgct tttcgaaacg cacttcgtgc ggataactca acaaaatatt tctaaaatgc
     1621 acgatggcat cgaaagcatt tgagtttttg tttctcagaa ttggttttga aaacacgctc
     1681 tgcatgccgc cttcgtgcag gtaaatgctt ttgagccctt cgcaaagtgt gattgcatca
     1741 aacgaattga aagtgcgata tacaaagccc tgcagctttt tcagatcttt ttgggtgtgt
     1801 cctgtcacaa attgaaatgg cacattatcc atgcgctgca taagttgttt tgcaccggag
     1861 ataatcgctt tgcggtttcc ccacgcaagc agcgcggaaa ataaagctga aatttcgata
     1921 tcttcttttt tcgaaaacaa atgcggaatt gacacagggt ctgtttcaac aaacgaaggg
     1981 ttgttgtaaa agtctgcttt ttcgttgagc cagtctgcaa ttttcgagtc tatctgatta
     2041 ttcattacaa cgagtcaata tatgtccacg aattacacaa attgcacgaa aatacttttt
     2101 tatttgaacg aatttacacg aagaataaaa aatctttgca ggcaaagatt ttttattggc
     2161 ttaagaaatt aatttgctca atgtggttgt tgaaatttat ttgactagtc ttttgtgttc
     2221 aagacttgac gctccaaaat taatcagcat tccaagttta aatcctgaag ctttcaagta
     2281 attcagcact acactttcgt gatccgaaac aagttgagac aaggccttta attcaactat
     2341 tattttttcg tagcaaacaa aatcagcaac gtaggatttt tttaattgta cttctttgta
     2401 tttgatgaat aaaggttctt catgcttgta aggaattcct cgtttttcaa attcaatcat
     2461 caaagcttca tggtaaacag cttcaagaaa accattgcca agtgttttat gaacttccat
     2521 tgcagcgcca acaatgctga atgattcgtc tttgtatatt atttctttca tgttttttta
     2581 ttccaaagat aaaatattac aaaacaactg actctccggc ccttcgtgaa atgatgtatt
     2641 tgcgactggc aaatgcatca ttttttcgta ataatcctga gatgtaaaga gcttctatga
     2701 gacagtggca ggcgcttaga ctttcatgta attcgtggat aaaaaaagac accagtattc
     2761 ttccctgtgt cagccttgcc agtcagataa aatccaggtc agcatatctt ttgtcaacct
     2821 tagctggctg aagtttaccc cgccgttcat gcttcgacgg agtttatcct gcgaaacgca
     2881 gggctcagca tgacagcgag cggggctaaa atgtcgggaa tgcgaaaata aaaaaagccc
     2941 cacctttcgg aggagctttt tgcaagtagt aatgaagttg ttttgttatt atttcactgt
     3001 gtaaggcaca atgatttcgc gtccgatacc aaccgctttc acattgtcgg gaatgagatt
     3061 gtggtggcgt gttggcaagg atttttttag acctgcttcg ataaactgat ctgttacaac
     3121 cggcttcact ttaaggaaac cacccaaaac aatcatattg aaaattttgg acaatccaag
     3181 atcggaagct tttttggttg caaggatttc ataaatctgc acatcttttc ttgtaggtgg
     3241 attaacgatg ccattgggat caaaaatcag aacaccacca ggtttcaccg agctttcgaa
     3301 tttatcgagt gattgctggt tgagaataat ggcagtatcg aactggttaa ggatcggcga
     3361 gctgatgcgg ttgtcgctga taataacggt aacgtttgcc gtaccaccgc gcatttcggg
     3421 accataagaa ggcatccaac tgacttcgag gccttgcatc attccggaat aagccaggat
     3481 tttacccata gagagaacgc cctgtccgcc gaaacctgct ataatcattt cttcagtcat
     3541 agtttgctgt tttaggatta ctttttagtg accatttgtc catcaacttt gatatcgccc
     3601 agcggataga aggggaacat attctgttcc atccattcat ttgccgcaac tggagtcatt
     3661 ttccagccag agttgcaatt tgaaacgact tcgatgaaag aaaggccttt gttgtttttc
     3721 tgattttcga aagcctgacg aacagctttt ttaaatttgc gcacattgcc aggtttatga
     3781 actgcatgtc tggctacaaa ataagttcca gggagctgag cgaccaattc ggtcattctc
     3841 aaagggtttc ccatcatttc aacttcgcgg ccataaggac tggtacttga tttcataccc
     3901 ggcaaagtgg ttggagccat ctgaccgccg gtcataccat aaataccatt gttgataaaa
     3961 ataataacaa tattttcacc ccggttgcag gcatgaatgg tttcggccgt accaatagcg
     4021 gcaaggtcac catcgccctg ataagtgaaa atatattttt cgggccacat gcgtttaagt
     4081 gctgtggcaa gggccggagc tcggccgtga gctgcctgct gcatatcgat atttagaaat
     4141 tcgtaggcaa gtacagaaca accaacagga gcaataccaa tggtgtcttt ctgaattccc
     4201 atttcctcga taacctcggc aactacacga tgcgcagtac cgtggccgca accaggacaa
     4261 taagagaaaa ccttatcggt taacaaagga gtttttgaaa aaaccttgtt ttcgggtttg
     4321 attatgtctt tccagacgtc taaagttaca tctgccatgg tattatcctc cgattatttg
     4381 ttttttaaga gcatcgacaa cctgagtcgg agtatgaatc ataccaccga tgcggccata
     4441 atgatcaact ttaattttgc cattgactgc cagtcgaaca tcttcgacca gctggccaag
     4501 actcatttct actgccagca ttccctttac tttcgatgca agccgattga gttcggcttc
     4561 agggaaagga tacagggtaa tgggacgatg aagcccaacc ttaatgcctt cggcacgggc
     4621 aagatcaact gctttctgac agatacgggc actggaacca taagcaacaa aaagatattc
     4681 ggcatcatcg gtcatgatgt tttcgaacat aacatcctgt ttcatgcttt cgtatttctt
     4741 gccaagcttg attacatgtt cctcctgttt ggcagaatcg aggtcgagcg aagtgatgat
     4801 gttccgttcg cgatcaggag ttttaccagt ggtagcccag ggacattctt tgataatttc
     4861 agcttcggaa cgacgcggaa tcgggtcgaa gagttcgact ttttccatca tctggccgat
     4921 aacaccgtcg ctgagcaaca tggccggtgt gcgatatttg aaagccattt cgaaaccgat
     4981 tttgacaaaa tcggacattt cctgaacgct ggcaggagcg agaacaaaca tgcgataatc
     5041 gccatgaccg ccacctttaa ctgtttggaa atagtcgcac tgactgggct ggatggtacc
     5101 aagaccgggg ccgccacgaa ccacatttac caaaagacag ggcaattcgg cgcccgccat
     5161 ataagaaata ccttcagcca taagactgat accaggactg gaagaagaag tcatcacccg
     5221 ctttcctgaa ccggcgccac catacaacat attgatggaa gcaacttcgc tttcagcttg
     5281 cagaacaacc attccggtgc gttcataagg tttttcagcc ataagatatt ctatcacctc
     5341 agactgaggg gtaatcggat aaccgaaata tccgtcgcag ccagcacgaa tcgcggcttc
     5401 agcaacggcc tcatttccct tcattaattt taattcaccc attgtttatg gtcttttaat
     5461 tattcaactt tttttgagta aacagtgata actgcatcgg gacaaactgt tgcacagttc
     5521 atacagccaa tacaggcatc ctggtaaggc tcagcataaa aataaccctt gatgtttact
     5581 ttatccccca gccttataac ggaattaggg caaaccggaa cgcaaacgcc acagcctttg
     5641 caccgatccc tgtcaattac aatagcacct ttaacttttg ccataacatg tttttttgaa
     5701 agattcttgg cgaaattata cattttttat ttatcagcac aattttcaac gttatttaaa
     5761 agaccttcgc aaatccgcat ctgatgcaga acagaggaat catgtaaaat tttttaaaaa
     5821 ataatctttt aataatggct ttttgaataa acaaataatt cattcgacta catttttcat
     5881 tttattcaag aaaaagaccc ccgaaaaata actttccgga ggtctgatat aatttaacag
     5941 tcgtcagttc tgactcacaa aagcttctgt agcagcatga aaaccgacga aactcaaatc
     6001 aacaaaaatg ggtttttgga gaaacccatc catcaaagac caaaggcctt ttttatgtcg
     6061 tcaacacgat ttaatttttc ccaggcaaaa aaggtaattt ttttcatcca tttttcaccc
     6121 tgtggtgttt gtttttttac cgcgcctgta tgaagttcag acacctcaac ttcctgttca
     6181 taggtcgaac ggccgaaatg tccgtaagca gctgttggag tataaatcgg attacacaaa
     6241 tcgtattcct tcagaatggc ataagggcga agatcgaaaa gaccacgcac gatgttgctg
     6301 atttccgcat cggtcatttt cactttagct gtaccaaatg tattgacatt gaagcccacc
     6361 ggttgggcaa cgccaatagc ataggccacc tgaaccaaaa cttctttggc aactccagcc
     6421 gccacaagat tttttgcaat atgccgtgct gcataagctg ccgaacggtc aactttagat
     6481 gggtcttttc ctgagaatgc gccgccacca tgagcgcccc gtccgccata ggtatcaaca
     6541 ataatctttc ggccggtaag accggtatcg ccgtgtgggc caccaattac aaattttccg
     6601 gtaggattga cgtgcagggt gaaatcattt ttaaaaagat ttttcacgcg ttcaggtgta
     6661 tccttagatt caataacacg tgggattaaa atgctttgaa catcacttct gatttgatca
     6721 agcgatacat tttcatcgtg ctgtgttgaa ataacaatcg tatcgatgcg ctgcgcaacg
     6781 cgattgtcgt catattcaat agtaatctgc gatttggaat cagggcgcag ataagtcatc
     6841 aatttgcctt ccttgcggat atcagcaagt ttctgaacaa aaatatgagc cagctcgacg
     6901 ggcatgggca tataattatc catttcgtcg caggcatagc caaacatcat tccctgatct
     6961 ccggcaccct gttcttcttc tttttcgcgg tcaactccct gattgatatc gggtgactgt
     7021 ccatgaatgg acgaaatgat gccacaagaa ttggaatcga agctgtattc gggcttggta
     7081 tagccgattt tctgaataat atgtcgggcg gtttcctgct ggtcaatcca gccggtagtt
     7141 gttacttcac ccgccaggac aacaagtcct gtggttacga gtgtttcaca ggcaacacgc
     7201 gattttggat cgtggcgcaa aaattcatcg agcaatgcat ccgaaatctg atcagctact
     7261 ttgtccggat gtccttcgga aacggattcc gatgtaaaca aaattcccat ttctttcgtt
     7321 ttgaagatta ataaatcagt taaaaaaagt gaatgggttt gattgcgtgc gcctaagctc
     7381 tacaatgttt tagcattttt tagcgtggtt gcaagcaatc aaatctttcc acagcgcaaa
     7441 agtacaattt tttttgaaaa tggaatatgt gtgggctgat aaatatcagt ggaggggaat
     7501 acaatgtggc tcttcgacgg agtctatcct tctatcgact cttcgacgga gttttccccg
     7561 ctgccccctt cgatactttt ctttgcttcg cttcgaaaaa ctcaggatga cagcggcggg
     7621 gttcagagtg acttttaagc gagagctgag gatgaaaaga caaaagataa aagtgtatta
     7681 gctttcagca gtctgctatc agccatcagt agtagcccaa cgcagcggag cggagcctca
     7741 tggcctattc cacatgccct gtgcgttgtg ctcctgaggc atatttccaa agcacaaaaa
     7801 aagccgcaca aggcggctcg gaaaatatca gtttggattt aaaaaatact gcaatttttt
     7861 ccaccaaaat aaaatttaat accacccatg aaagaaccaa aagcatcatc ggaattggta
     7921 ataccttcgc gtgcatcgag caaatcggtg ggcgtattgt accatgaata ttcgccatag
     7981 attgaaagca tcggcagtat atttatttca gatccaaatc caaaaatata ggtgaatacc
     8041 atttcgcgtt tatccattcc gacttcactg acaacaacac cagtagttgc aatgcgctgc
     8101 tgagcgcgat agaatgtggc acctgctcca agacgtccgt agatatcaaa ggggagaaat
     8161 ttacatgatc ggtttgtaat gtttgtaagt ttgaacaaat gcgcctgaaa atcagctgaa
     8221 agcgtccaga aattgcaggt aaatgcctga tcggttggat aatcattggt atagaacatt
     8281 tttgttccat cgaggcgacc atcgaaaacc tcaaggtttg agctgaatgc gggagcccag
     8341 tgttttccga agcgaatggc gtaaccgcca ctgacttcgt tatattgttc agtaaaatga
     8401 aaaccatcgt aagaggttac atcggtataa ggcaacaact gaccgccggc aacaccagca
     8461 aaccatcctg tttgcatgtc atagcattgt gttccttcgg atttaccttg tgcattaatg
     8521 tatgcaacaa aaaataaact gcagataata gataacagaa ttttcatgat gttggatttt
     8581 aatgcacaaa tgtaaaaata aaaatcaata tcattcactt ttgcgcataa actctcatat
     8641 ttatatatat ggaatatgaa aagaaatgta aaataatgat gggggaaaaa gaagtttcgc
     8701 cacaa
//
LOCUS       DAOPKE010000111         8274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9175_length_8274_cov_11.003772, whole genome shotgun sequence.
ACCESSION   DAOPKE010000111 DAOPKE010000000
VERSION     DAOPKE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_8274_cov_11.003772"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1578)
                     /gene="mnmE"
                     /locus_tag="PKY63_11200"
     CDS             complement(187..1578)
                     /gene="mnmE"
                     /locus_tag="PKY63_11200"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPF01226.1"
                     /translation="MIPNLSETICAIATPPGTGAIAVVRLNGPKSHSILESIFSPAKK
                     NGSFPELRHSYYGEIVDGNEVIDDAIVVFYKNPGSYTGEDAAEISCHGSLFIQNRLLQ
                     LLISKGARMAEPGEFSLRAFVNGKMDLLQAEAVDDIIRSRTGAAHKLAVKQMRGEYSK
                     KLSELRKQLVDLTALMELEIDFSEEDVEFADRNRLRETCAELVNEVSLLADSFKAGNV
                     LKHGIPVAIVGEPNVGKSTLLNKILREERSIVSDIPGTTRDFIEDTLTVGNNTFRFID
                     TAGLRTTTDSIESAGVERTLVKASEASVILLLFDAADCEEDRVQSGIEELKSRIEDFD
                     DKKLILVINKIDLLVNAPRHLRELLEYDVVFISAKRDENIGELMEKLTNAVSVSENTP
                     DIVLTNVRHYEAFSVIGSLLQQAIEHIDSGMPQDLTSSFLHQALHHIGLITGEVSNED
                     VLDSIFRNFCIGK"
     gene            complement(1758..2906)
                     /locus_tag="PKY63_11205"
     CDS             complement(1758..2906)
                     /locus_tag="PKY63_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF01227.1"
                     /translation="MEALILKSKMGFNLRPIDNRRMTNKASDIDFFDDSGNINSRVLY
                     YRLFESIAFVKQSYTRGDAHEKIAERLKNSSYGPNVEILFHMNMSKHAFTPDPVEENQ
                     IGQEVIYKVGEGIIIHLLDQSYALLYPSLMSEEEVKHLLHIIEGDVAERERKKLHKFS
                     MITNQYGFDLEYFEIKHYEIDLLKQYNDDFMAVDAKINAFLNEPKSSGIVLLHGVYGS
                     GKTTYIRHLINSTEKNFIYVPLNMVGSLNEPEFLPFISQYHDSILIIEDCENLIQSRK
                     NNGSSSSLVNLLNMGDGLLGDALRLKIICTFNADLKEIDEALLRKGRLVVRYFFDKLC
                     AAKSVALQWAIDSKPIVEESMTLADIYNTDENDLVGDAHKKSIGFKKN"
     gene            3556..5505
                     /locus_tag="PKY63_11210"
     CDS             3556..5505
                     /locus_tag="PKY63_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPF01228.1"
                     /translation="MGYIQFDKKQLINLEYVLSKEILRTNRAGAYASTTIIGCNMRKY
                     HGLLVCPQPAIDNELHVLLSCIDESIIQHDSEFNLSLHRYPGSWSPKGHKYLVDFDSE
                     PTVALFYQVGGVLLKKEMLFGVDDRILIRYTLLDAHSPTKLRLRPFMAYRQRHNLSKA
                     NEFVDRKYSVINGGIKTRMYKGYSYLHFQFSRDAGYVHVPDWYYNFEYPKDLERGYDG
                     HEDLFTPGYFEVDIVKGTPLIISVGTSEMDPKNLSRTFTRELNKRTPRSSYWNCLNNS
                     ANQFIVTRDKKTEIIAGYPWFGRWGRDTLISLPGLTLTRDMDLTFKSAIDSLIMDMNG
                     PLLPNVGSGKDAAFNSIDAPLWLFWTLQQYVLMAAASEKNIWDDYGPTMKIVLNGLRN
                     GKVNGIYMKPNGLLYALIPGKALTWMDAIVDGKPVTQRGGLAVEINALWYNAIMFALQ
                     LAEKCGDDSFVLNWKAVAEKIPVSFKDTFWSKEKGYLADFVDDDYKDWSVRPNMVFVT
                     SLPFSPVSEKIRQLIIDKIRQELLTPRGLRTLSPIDSKYKGNYEGTIRDRDLAYHQGT
                     VWPWLLSHFVEGFLKIYGKDGVEQVRPLYDNMEPTLFEHGVGTISEVYSGDPPHRPGG
                     AISQAWSVSEMMRIKWLIDKYSGES"
     gene            5502..6779
                     /locus_tag="PKY63_11215"
     CDS             5502..6779
                     /locus_tag="PKY63_11215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF01229.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACFGLTRSLARMHTDVLFVVPRAF
                     GDEDTSMVKLVNASDVEVDLEKSEYSEFWKRIQYMSVASDIIPYVGPVEYSHTHVKKL
                     KKEFHSNSIFSHRYTFSGSYGKDLMYEVSRYALIASGIAASNNFDIIHAHDWLTYPAG
                     IAAKQVSGKPLVIHVHATEFDRSGEHVNQRVFDIEREGMMQADRVITVSNLTRNIVIE
                     RYGINPDKVFTVYNGADAFQAPAIERIEKPFPEKVVTFLGRITFQKGPDYFVEAAKKV
                     LEKDENVRFVMAGSGDMLPRIVRMVSKHRMGHKFHFTGFLKGNDVDKMFSYSDVYVMP
                     SVSEPFGISPLEAMRSSVPVVISRQSGVAEVLRHALKVDFWDVDAMADSIYGLLNYPA
                     LSRMFSRFGRNEVEDLKWDNAAASVLSVYQHLV"
     gene            6790..8037
                     /locus_tag="PKY63_11220"
                     /pseudo
     CDS             6790..8037
                     /locus_tag="PKY63_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
BASE COUNT         2234 a         1752 c         1921 g         2367 t
ORIGIN      
        1 aggttttcca ggagatcccg gcacctcatg aacactgaat cttgaacctg tctgactgcc
       61 tgttgcagtt aaactggcca ggcaggcaag gaagtgagaa tattgaatga atttacttct
      121 cacttcatat ttccttgttc tttgttcaat attcagtttt gctgccttcg tgcctttgtg
      181 gccttactac ttgccgatgc agaaattccg gaaaatcgaa tccagcacat cttcgttcga
      241 cacttcgccg gtgatgagac caatgtgatg cagcgcctga tgcagaaaac tcgaagtcag
      301 atcctgcggc atacccgaat cgatgtgttc gatggcttgc tgcagtaagg atccgattac
      361 gctgaatgcc tcataatgcc ttacatttgt gagcacaatg tccggcgtgt tttccgaaac
      421 actgactgca ttggtcagct tttccatcag ctcgccgatg ttttcatcgc gttttgctga
      481 aataaaaacc acatcgtatt caagcagctc gcgcaaatgc cttggcgcat tcacaagcaa
      541 atcaatttta ttgatgacaa gaatcaattt cttatcatca aaatcttcga tgcgactttt
      601 caattcttca atgccgctct gaacgcggtc ttcttcacag tcggcggcat cgaaaagcaa
      661 aagaataacc gatgcttccg atgcttttac caatgtgcgc tcaacgccgg ccgattcgat
      721 agagtcagtt gtagtgcgca atccggcggt atcaataaag cggaaagtat tatttccaac
      781 cgtcaaagta tcttcaataa aatcgcgcgt ggtacccgga atgtcgctca caatcgagcg
      841 ttcttcgcgc aggattttat tcagtaaggt tgatttcccg acattcggtt caccgacaat
      901 ggccacagga atgccgtgct tcaatacatt gccggctttg aatgagtctg ccagcaaaga
      961 gacctcattc acaagttcag cgcaggtttc gcgcaggcgg ttgcggtcgg caaattcaac
     1021 atcttcttcc gaaaagtcga tttcgagctc catcagcgcg gtcaaatcaa ccagttgttt
     1081 gcgcagttcg ctgagctttt tcgaatactc gccgcgcatt tgcttcacgg ccagtttatg
     1141 tgcagcgccc gtacggctgc ggatgatgtc atcaaccgct tcggcctgaa gcagatccat
     1201 tttgccattc acaaaagcgc gcaaagaaaa ttcgccgggt tcggccatgc gtgcgccttt
     1261 gctgatgagc aactgcagca gccggttctg aataaataac gagccatgac atgaaatctc
     1321 agctgcatct tcgccggtgt aggagcccgg atttttgtaa aacaccacaa tggcgtcgtc
     1381 gatcacttca tttccgtcaa cgatttcgcc gtaatagctg tggcgcaatt cgggaaacga
     1441 accgtttttt ttcgcaggac tgaaaatgct ttccaggatt gaatgcgact ttggaccgtt
     1501 caaccggaca acagcaatgg cgccggttcc gggtggtgta gcgatggcgc agatggtttc
     1561 tgataaatta ggtatcatag agcaaagata agaaatagtc gtcaggcata agtcgttagg
     1621 cgtaagtccg ccgcggcaga ttagacgggg cggggaagag gcactgttac ggatgacata
     1681 tccgcgccag tgcgatgggc tgtgtggctc agcgcaataa ccggcagacg ttaaaatgta
     1741 ggaaaactac ccaacaatca attcttcttg aaaccaatac tttttttatg ggcgtcgcca
     1801 accagatcgt tttcatcggt gttgtaaata tcggccagtg tcatggattc ttcaacaatc
     1861 ggcttgctat caatggccca ttgcaaggca acagattttg cagcacatag cttatcgaag
     1921 aaatagcgca caaccagtct tccttttcgc agcaatgctt catcaatttc tttcagatcg
     1981 gcgttgaatg tgcagataat tttcaggcgg agtgcatcac ccagcaagcc atcgcccata
     2041 ttgagcaggt ttaccagcga gctggacgaa ccattgtttt ttcttgattg aatcaggttt
     2101 tcacaatctt caataatcag aatactgtcg tggtattgtg aaataaaagg cagaaattca
     2161 ggttcattaa gcgatccgac catattcagc ggaacataaa taaaattctt ttcagtactg
     2221 ttaatcagat gtctgatgta ggtcgttttt ccgcttccat aaacaccatg cagcaataca
     2281 atcccagacg attttggttc attcaggaac gcattgattt ttgcatcaac agccataaag
     2341 tcatcgttgt attgtttcaa aagatcgatt tcgtaatgtt taatttcgaa atattcgaga
     2401 tcaaaaccat attgattggt aatcatgctg aatttatgaa gctttttgcg ctcacgttcg
     2461 gcaacatcgc cttcaataat atgcaacaaa tgttttactt cttcttcact cattaaagat
     2521 gggtagagta gcgcatatga ttgatcgagc aaatgaatga tgattccttc accgacttta
     2581 taaatgactt cctgtcctat ttggttttct tcaaccggat ctggagtaaa ggcatgttta
     2641 ctcatgttca tgtgaaacaa tatttcaaca tttggtccgt atgaagaatt cttcagacgt
     2701 tcggcaattt tttcatgtgc atcacctcgg gtatagcttt gttttacaaa tgcaatactt
     2761 tcaaacaacc ggtaatacag tacacgacta ttgatgtttc ccgagtcatc aaaaaaatca
     2821 atgtctgatg ccttgtttgt cattctccgg ttgtcaatcg gacgtaaatt aaaacccatt
     2881 tttgatttca aaattaatgc ttccatgtgt ttctttgttg cttgttgata actatttctt
     2941 gttcataacc ctgctcatgg atgcagaatt gaaattaata cataaaatct tctataaatg
     3001 gagtaaccgc gggatcgttg ggtgctttgg cctctttcgc cccgacgtct gcctttccag
     3061 tcagataaaa ttccaggtca gcaggtcaat tgccaatcca cccttgctga cgctaaaatg
     3121 ttggggaggg cggtgtccaa cgcttagccc cgctgtcatt ctgagtgttt gaagaagtga
     3181 ctttgaaaac aacagacttc aaatgtatcc ccgccagctg cgcagcgggc agggaagaac
     3241 ggcagcgggt tttaacaagt cgcacttcga tacgatgaag gcccctggac cataatgctg
     3301 aatgtttcat caatcagtgt gacagatgtt ttttgggcgc gcatctcctg acctatacgt
     3361 caacaggttt tgcgagggac actcaaatgt caggaagcat cgcaattttc attgacactg
     3421 cactcgcatt ccaggcaact ttaatattaa tattatatga ttttatgagg aataatctaa
     3481 ctcacattga tattctgaaa gaaattcact aattttgcaa actaatttaa aaatttaaag
     3541 tacaaactgc aagatatggg gtacatacaa ttcgacaaaa agcagctaat taaccttgaa
     3601 tatgttctca gcaaggaaat tctgcgtacc aacagggcgg gggcttatgc atcaacaaca
     3661 attattggtt gcaatatgcg taaataccac ggtcttcttg tttgtccgca gccggctatc
     3721 gacaatgaat tgcatgtatt gctttcatgc attgacgaaa gcattattca acacgacagt
     3781 gaattcaacc tttcgctgca tcgctatcct ggctcatggt cgcccaaagg acataaatat
     3841 cttgtcgatt ttgattccga accaaccgtc gctcttttct atcaggtagg tggagtgctg
     3901 ttgaaaaaag aaatgctttt tggtgtcgat gaccgcattt tgattcgtta tacgctgctc
     3961 gatgcgcata gcccgaccaa acttcggctg agacccttta tggcttatcg ccagcgacat
     4021 aatctgagta aggccaatga atttgttgat cggaaatatt ctgtcatcaa tggtggtatc
     4081 aaaacccgca tgtataaagg atacagctat cttcattttc agttttcacg cgacgccgga
     4141 tatgttcatg tgcccgactg gtactataat ttcgaatatc cgaaagatct tgaaagagga
     4201 tatgatgggc acgaagatct ttttactcct ggctatttcg aggttgatat tgtcaaggga
     4261 actcccttga ttatttcagt tggtacatcg gaaatggatc caaaaaatct gtcaaggact
     4321 tttacgcgtg agttgaacaa gcgcacacca cgcagtagct attggaattg tttgaataat
     4381 tcggctaatc agtttattgt tacccgcgat aaaaaaacag aaatcattgc tggctatcct
     4441 tggtttggac gctgggggcg cgatacgttg atttctcttc caggactcac tctcacgcgt
     4501 gatatggatc tgactttcaa atcggccatt gattcgctta ttatggatat gaatgggcct
     4561 ttgctgccca acgttggttc aggaaaagat gctgcattca attccatcga tgctccattg
     4621 tggcttttct ggacattgca gcaatatgtg ctgatggctg ctgccagcga aaaaaatatc
     4681 tgggatgatt atggtccaac tatgaaaatt gtccttaacg ggcttcgtaa cgggaaagtg
     4741 aacggcattt acatgaaacc caatggtttg ctttatgctt tgattcccgg aaaagcgctt
     4801 acatggatgg acgccatcgt tgacggtaag ccggttacac agcggggtgg actggctgtc
     4861 gaaatcaatg cgctctggta caatgccatc atgtttgctt tgcaactggc tgagaagtgt
     4921 ggcgatgata gttttgtcct taactggaaa gctgttgcag aaaaaattcc ggtttctttc
     4981 aaagatactt tctggtcgaa agaaaaagga tatctggccg attttgtcga tgacgattac
     5041 aaggactgga gtgtacgtcc taatatggtg tttgttactt ctctgccttt ttcgccggta
     5101 agtgaaaaaa tcagacagct tatcattgat aaaatccgtc aggaattgct tactccacgc
     5161 ggtttgcgga cattaagtcc gattgattca aagtataaag gcaattatga aggtaccatc
     5221 cgagatcgcg atctggccta tcatcaggga accgtctggc catggttgct tagtcatttt
     5281 gttgaaggtt ttttgaaaat ttatggaaaa gatggtgtgg agcaggtaag accattgtat
     5341 gataatatgg agcccacatt gtttgaacat ggagtaggta ctatcagtga agtttatagt
     5401 ggtgatcctc cgcatcgtcc gggtggagcc atttcgcagg cctggagtgt ttcggaaatg
     5461 atgcgcatca aatggttgat tgataaatac agcggagaat catgagagtt ctgatgtttg
     5521 gatgggaatt tcctccgcat attaccggag gtcttgggac tgcctgtttc gggctaaccc
     5581 ggtcgcttgc gcgtatgcat accgatgtgc tttttgtggt gccgcgtgct tttggcgacg
     5641 aggataccag catggtgaaa cttgtgaatg cttcggatgt tgaagtggat ctcgaaaaat
     5701 cggaatacag cgaattctgg aaacgcattc agtacatgtc ggtcgcatct gacattatac
     5761 catatgtcgg gccggttgaa tattcacaca cacacgtcaa aaaactcaaa aaggaatttc
     5821 acagcaacag cattttttcg caccgataca cattcagcgg ctcctacgga aaggacctga
     5881 tgtacgaagt gtcgcgctat gctttgattg caagcggtat tgctgcgtca aacaatttcg
     5941 atattattca tgcacacgat tggcttactt atcctgccgg tattgctgcc aaacaggtga
     6001 gtggaaagcc gctggtcatt cacgttcatg caactgaatt cgacaggtcg ggtgagcatg
     6061 tcaatcaacg agttttcgat atagaacgtg agggaatgat gcaggccgat cgcgtgatca
     6121 ctgtttcaaa tctgacacgc aatattgtga ttgagcgcta tgggattaat cccgacaagg
     6181 tgtttacggt gtacaacgga gccgatgctt ttcaggcacc tgcgattgaa cgtattgaaa
     6241 aaccatttcc tgaaaaggtg gttacatttc tggggcgtat cacttttcag aaaggtcctg
     6301 attattttgt tgaagcagcc aaaaaagttc tcgaaaaaga tgaaaatgta cgctttgtca
     6361 tggcgggttc gggcgatatg ctaccgcgta ttgtgcgcat ggtttcaaaa cacaggatgg
     6421 gtcataaatt ccattttacc ggatttttga aaggcaacga tgtcgataaa atgttttcgt
     6481 acagcgatgt atatgtgatg ccaagtgtat cggaaccttt cggcatttcg ccgctcgaag
     6541 ctatgcgcag cagtgtacct gtggtcattt cgcggcaatc gggtgtggcc gaagtgctgc
     6601 gacatgcatt gaaagtcgat ttctgggatg ttgatgctat ggccgacagc atttacgggt
     6661 tgttgaatta tccggcgctt tcgcgtatgt tttcacgttt tggccgcaac gaagttgagg
     6721 atttgaaatg ggacaatgct gcggcatcgg tccttagtgt ttatcagcat ttagtatagg
     6781 aggcgtttta tgagatcaat ctgtttctat tttcaggtac atcagccgtt ccgtttgcgg
     6841 acgtatcggt tttttgaaat cggtaataat caccattatt ttgatgatta tcagaatcga
     6901 tatatcctgc gccgtgtggc tgataaatgc taccttccca tgaataaact cctgcttgat
     6961 ctgattaagg aattcggaca acaatttaaa gtgtcgtttt ccatttcggg tgttgcgctt
     7021 gagcagtttt cgaaatacac acctgaggtg attgacagct acagagcgct tgcagaaaca
     7081 ggttgcgtgg agtttattgc cgaaacctac agccattcgc tggctgcttt gcgcaatgaa
     7141 caggaattca gggctcaggt aaaacggcaa cagcaattgc tgaatgatct gatcggatat
     7201 cagagcacga ctttccgaaa taccgagttg atttattcaa acgaaattgg cgacatggtt
     7261 tatcgtatgg gctacgatgt catgctcacc gaaggcgcca aacatgtgct gggctggaaa
     7321 agtccgaact acatgtattg caacgcgcag aatccgcgac tgaaattgtt gctgaaaaac
     7381 ttccgtctga gcgatgatat cgcttttcgt ttctcgcagc aaagctggga aggatggccg
     7441 ctgaccgctg aaaaatttgt tgactggctc aatcaggtag atccgcgcga agaagtggtg
     7501 aatcttttta tggattacga aacttttggt gaacatcagt ggcctgaaac cggaatattc
     7561 gattttatgc gggctctgcc gggaactgtt tttgcgaaaa gtaatttttc atttaatact
     7621 ccatcagaac tcggacatat gatgcagccc gttgcgccgg ttcatgtgcc gtttccgatt
     7681 tcgtgggctg atgaagaacg tgacctcacg gcttggctcg gcaacgattt gcaggatgat
     7741 gctttcgata ctttgtacag tttgtcagaa aaaatggagt acgtgatgtc gtattccaaa
     7801 agtattgaca ccatgactga agccggcgtc gctgctcaca aagtgagtgt tgatgagttg
     7861 aataaattgc acgatgattg gttgaaactg cagaccagcg accattttta ttatatgtgc
     7921 accaagtggt ttgccgatgg tgatgtgcat aaatacttca atccgtatgg cactccgtac
     7981 gatgcgtata tcaattttat gaatgtgctg agcgatttta tgttgcgcct gagtcagcat
     8041 tcgtttttga atagtgtgcc agaaccggct ctggtagccg aacagccaaa gtcggataca
     8101 aagaaggtgg tcaaactcac tgttggaaaa aagacgactg caaaaaaatc cactaccaaa
     8161 aaagcaacag agaagaagag cactacaaag aaaacagcgg agaagaaaac aacaaaagca
     8221 aaagcagcaa gaactaaaat cacagtgaag aaaactgctg cgaagaaaaa gccg
//
LOCUS       DAOPKE010000112         8203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9297_length_8203_cov_12.246195, whole genome shotgun sequence.
ACCESSION   DAOPKE010000112 DAOPKE010000000
VERSION     DAOPKE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9297_length_8203_cov_12.246195"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..711
                     /locus_tag="PKY63_11225"
     CDS             187..711
                     /locus_tag="PKY63_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01230.1"
                     /translation="MEVLAHYLRLAFIAAIVMLLIAGIMYLAIKQKSKKKNDKTEVAG
                     RLHFYKMIVIASAVFIPLYLIAYVVYLKDVPVLKYTTDAQFESAYLKNFNNNTLNDST
                     RNLFYDQSMDFLKNRHHDKIFFDDFVFDKTDSIELSFILYYIEHPGLNDSTKLEIRNR
                     VQTVQDVEKYLISK"
     gene            817..1173
                     /locus_tag="PKY63_11230"
     CDS             817..1173
                     /locus_tag="PKY63_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01231.1"
                     /translation="MTITGFDFYKSDCITEGAKLNNPAILDTLTDFLNKFPELCVRLD
                     FHAPCCCKSEEYYLDQTSLIAIMIKDILVSNGIDEDRLSTLGAGTSNPLFNCICGECP
                     EYMMILNRRLKVVIIQ"
     gene            1213..2022
                     /locus_tag="PKY63_11235"
     CDS             1213..2022
                     /locus_tag="PKY63_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01232.1"
                     /translation="MKKIALLTLLLMPLALSAQKKYSKEEIVEITDSILIEGNLLYQY
                     ERAAWMTTDLARADKQLSRLFKSYLIYKDEDTIKGLILNNSNLVIATYLFFEDFTKEY
                     SSDFTKRDLTPFENEIFIARFEILQKITEGKYPVTCPEGYSLNMILIPFNGGYKLYFI
                     TGTSKNGVIPFGNDYVFFAGSDYTIHDWRKFHSRLIPVEGGSGENKISSSVHSHLKNE
                     PFITATDICTFKLYAGYVGLKSFQVYSPALSLYFRYSLESDSIETSKKMFD"
     gene            2063..2407
                     /locus_tag="PKY63_11240"
     CDS             2063..2407
                     /locus_tag="PKY63_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01233.1"
                     /translation="MFSISFDDIIRFPKMPTADMLEQWKNNVIENAMLLIIRLTLWFR
                     AQISKVRPGAAIVRNIAPARTNSEVWNCYDVAVQTGLQQSPVRCRNPQSDNWWNISPA
                     TPPFVVLMNIRL"
     gene            2534..3283
                     /locus_tag="PKY63_11245"
     CDS             2534..3283
                     /locus_tag="PKY63_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPF01234.1"
                     /translation="MKNYFEKQFELRYFELNKFGEAQATTILTLLEETAADHCWSIDH
                     SLYDLKYQNIGWVLLSGAIVMERYPLYKEKIIIRTWLSHYTPIRGLRENIIFDSRRNI
                     IGRSTGLWVFFDLNRRRPARIFDDIRDKWSSEEEKSTSIDITETIDPIVSGVYQKQFH
                     VNRFDIDTYQHVNNIRYLQWMSESIPDDIADHYFMHTLNGRFLREAQQDDTLISYTNP
                     LSESGHFEHSIRSANEGHFCAAAKTIWKKKS"
     gene            3935..4945
                     /locus_tag="PKY63_11250"
     CDS             3935..4945
                     /locus_tag="PKY63_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF01235.1"
                     /translation="MFSFKNDYAEGCHPELLKMLIDTNMSQQEGYGDDDFSGLARTRI
                     LQQLQCDADIHFVSGGTQANTVVLAAALKPFESVIAAESAHINVHEAGAIEATGHKIE
                     YASTPDGKLTPELIAPLLIKAEDHHMVKPRLVYISQSTELGTVYKLAEIEALSAFCKQ
                     NNLLLYADGARLGAALTSPAADFTLADMARLFDAFYIGGTKNGALMGEAIVLLNPDLK
                     RDFLFHLKQRGALLSKGRLAGCQFAALFSNNLFFDLARHSNNMALEMAAAIAKAGYPF
                     FVQPESNQIFPILPMTLIEKLSKEFGFYIWRKTDDQHAIIRLVTSWATPQSEVERFIE
                     NL"
     gene            4946..5746
                     /locus_tag="PKY63_11255"
     CDS             4946..5746
                     /locus_tag="PKY63_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01236.1"
                     /translation="MNRFYSYSLIAIVLFSANGLKVHAQYPGYFKLKTINGTVYNTDN
                     TPIDVEFISRYKLKITNCTDPDVDTLLRTLTLTRVSRDYLTHLITTSAKVVINVSDNI
                     GIAFSEGKYRFIAGICGPDKDQSHTLIPDLTSSLCPRKTFRHPVNVYAQSTITLFKGS
                     ISYVHTGVPELAEQNVQIFDLDSNRCIKTFSMDSVVVEKIMYPDMLYRNTRELYFFGG
                     THEVCHSTAENIAVNDSDLCEAMAFDMEKKLFKKRKAINRKKIIYPTK"
     gene            5945..7045
                     /locus_tag="PKY63_11260"
     CDS             5945..7045
                     /locus_tag="PKY63_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPF01237.1"
                     /translation="MLTNVHTKSLDELYRTPIVQQTAFWSVVKQKLGEKTLAVHYSSR
                     ASDLFGPVSYEKYINSDLLVIIKRVMPGYSMAYVPYGPELEPSAENQGAFLEELAECM
                     RSFLPKNCINIRFDLCWESYWSDGESLSEPSINAQELRFNINTNKWNFRKSMFNNLPS
                     STMFVDLKPDTRNIIDLMKPKTRYNIGLAERKNVVVKQMGIESLPLWYQLYEQTAIRN
                     NIFLNDIKYFEAVLTAQTNDARSPAEVILLMAEYDSMPLAAMFLIISGNRGSYLYGAS
                     SSEHRNKMATYALQWAAMNISKKHGCTEYDMFGVSPNPDPDHPLNGLYKFKSGFGGEL
                     FHRLGCWDYPLCNDQYQILKSIELNQMGFHLN"
     gene            7140..7598
                     /locus_tag="PKY63_11265"
     CDS             7140..7598
                     /locus_tag="PKY63_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01238.1"
                     /translation="MKHYSLFLISLMLFYLSACTQKGSQQNDDSNQLSYSIKRTDFVM
                     GTHKNFEWILQNDSVKVIEYPMSSDPDSMVCYKALSERQRIKLQSIMKTIDLTQMQDR
                     YSDDLVLGEGHSVYDISINGSSKHIYVYYVDVPELIKIDEFLYDVTADCK"
     gene            7944..>8203
                     /locus_tag="PKY63_11270"
     CDS             7944..>8203
                     /locus_tag="PKY63_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF01239.1"
                     /translation="MEGIDANGYEAATPIQEQAIPAILSGRDLIACAQTGTGKTAAFL
                     LPLTNNIITSEQNHRIKCMVIEPTRELAIQVDQQMQGFSYFT"
BASE COUNT         2435 a         1791 c         1666 g         2311 t
ORIGIN      
        1 gacagcttgt ccgcccatca attcacgaca gtgattgatg ccggtggaat tccacgtcag
       61 caatatttgt aatgtcattc tttgcaagct gacggtagaa tgtcagggat gggattatta
      121 aaaaaagtcg cacgaagtat ccctacttcg cacaacttca ctaattttac tgtgtaattc
      181 aaaatcatgg aagtcttagc tcattatctg cgtctcgcgt tcattgctgc catcgttatg
      241 ctgctcattg ccggcattat gtatcttgcc atcaaacaaa aaagcaagaa aaagaatgat
      301 aaaacggaag tggccggccg tctgcatttt tataaaatga ttgtcattgc ttctgctgtt
      361 ttcattccat tgtatctgat tgcctatgtg gtttatctca aagatgttcc tgtcctgaaa
      421 tacactaccg atgcgcagtt tgaatcggct tacctgaaaa atttcaacaa caatacattg
      481 aatgacagca cccggaatct gttctacgat cagtcaatgg attttcttaa aaatcgtcat
      541 cacgataaaa tatttttcga tgactttgtt tttgataaaa ctgattccat tgagctgagt
      601 tttattctct attacatcga acatcccggg ctgaatgaca gcacaaagct ggaaatcaga
      661 aacagggtgc aaactgttca ggatgttgaa aaatacttaa tcagcaaata aatgctgcga
      721 ttaaccgcat taatactaat gtttcagctt gtatgccaac aaaggactta cgctcagaat
      781 tcatccgaat ctttaagtaa taccgatcta atcatcatga ccattacagg ttttgatttt
      841 tataaatctg attgcataac agaaggagca aaattgaata atcctgcaat attagatacc
      901 ttgacagact ttctgaataa atttccggaa ttgtgtgtac gccttgattt ccatgcccca
      961 tgctgctgca aaagtgaaga gtattatttg gatcagacaa gtttaatcgc aattatgatt
     1021 aaagatattt tggtttcaaa tggtattgat gaggaccgat tatccacatt gggtgctggt
     1081 acctcaaacc ctttgttcaa ctgcatttgc ggagaatgcc cggagtatat gatgatattg
     1141 aaccgtcgcc tgaaagttgt gattattcaa taattcaata tatttgcttt taatttaata
     1201 tatattgctg ctatgaaaaa gattgcattg ctgactttgc tcttgatgcc acttgcacta
     1261 agtgcacaga aaaaatattc caaagaagaa attgtcgaaa tcacggattc aattctgatt
     1321 gaaggaaatc ttctttatca atacgaaaga gctgcatgga tgacaactga tctggctcgg
     1381 gctgacaaac agttgtcgag attattcaaa tcatatctga tttataagga cgaagacact
     1441 attaaaggtc ttattctcaa taactcaaac cttgtaattg caacttattt gttttttgaa
     1501 gattttacaa aagaatacag ttcggatttt acaaaacgcg atttgactcc gttcgaaaat
     1561 gaaatattta ttgctcgctt tgagatactc cagaaaatca ctgaaggaaa ataccctgta
     1621 acctgccctg aaggctattc tctaaatatg attttaatac cattcaatgg tgggtataaa
     1681 ttgtatttta tcactggtac ctcaaaaaat ggggtcatcc catttggaaa cgactatgtt
     1741 ttttttgccg gatctgatta taccattcat gactggagaa aatttcacag cagactaata
     1801 cctgttgaag gtggatctgg tgaaaataaa atttcaagtt ccgttcacag tcatctgaaa
     1861 aatgaacctt ttataacagc tacagatatt tgtacgttca agttatatgc aggttatgtg
     1921 ggattaaaat catttcaggt ttattctcca gcattatcgc tttatttcag gtattcactt
     1981 gaatctgatt cgattgaaac ttccaaaaaa atgtttgatt gaaataaata tctgaaatat
     2041 aaattcaaca tatctttgcc ccatgttttc aatcagcttc gatgatatca tccgttttcc
     2101 gaaaatgccc acggccgaca tgctggagca atggaaaaac aacgtgattg aaaatgcgat
     2161 gctgctgata atccgcctga cactgtggtt cagggcgcag atttcgaaag tacgaccggg
     2221 cgccgccatt gtgcgcaaca ttgctccggc ccgcaccaac agcgaagtct ggaattgcta
     2281 cgatgtcgcc gttcagaccg gtctgcagca atcgcccgtg cgatgccgga atccgcaatc
     2341 ggacaactgg tggaacattt cgccggctac cccgcccttt gtggtgctga tgaacatcag
     2401 actctgaaaa atttttcatt tccttccggc agacagctta ctgttttgcc gttgcgactg
     2461 tttatcataa gtcgctgaaa ccagggattg atgcatgttg tcaatctgtt gtgcaacaat
     2521 aaaaaaatga aaaatgaaaa attatttcga gaaacaattc gaactccgtt atttcgaact
     2581 caataaattc ggcgaagccc aggccacaac cattctgact ttgctcgaag aaactgcagc
     2641 cgaccattgc tggtcaattg atcacagtct gtacgatctg aaatatcaaa acataggctg
     2701 ggtgctgctt tcaggtgcca tcgtcatgga gcggtatccg ctttataaag aaaaaataat
     2761 catccgcacc tggctgtcgc attacacgcc catccgcgga ttgcgcgaga acatcatttt
     2821 cgacagccgc cgcaacatca tcggtcgctc aacgggattg tgggttttct tcgatctcaa
     2881 ccgccgccgc ccggcgcgta tattcgacga tatccgcgat aaatggtcga gcgaagaaga
     2941 aaaaagcacc agtatcgata tcaccgaaac cattgatccg atcgtgtccg gcgtgtatca
     3001 gaagcaattt catgtaaacc gcttcgatat cgatacctat cagcatgtaa acaacatccg
     3061 ctatctgcag tggatgagcg aatcgattcc ggatgacatt gccgaccatt atttcatgca
     3121 taccctcaac ggccgcttcc tgcgcgaggc ccagcaggac gacacattga tttcctatac
     3181 caatccgctg agtgaatctg gtcatttcga acattccatc cgctctgcaa acgaaggcca
     3241 tttctgcgct gcagccaaaa ccatctggaa gaagaagagc taacggttgg gcgtagtcta
     3301 tatgaaattt ctagttgagg ctgtctcaaa aagtcttcag cgacaaaaac aacattccat
     3361 cgtctggaag cgacgcagga gctggaggac atggaaccgt tgtttttgct gttgttacga
     3421 cggttccatg ttccggccgt acgtcatccc tcgaagaatt cgaaactctt cgaggagttt
     3481 taaactggcc ggaacggtgg aatgtcgtaa caacccgctg ttttgataca tcctcggcag
     3541 tataaataat taagtctacg tccgacctca aaatgatcga tcacatcata tttttttcta
     3601 atttcgcttt aattgaactt cgttgctcct ctgaaggctc agccactgac aactgaatac
     3661 tgactactga aagctgacta ctcaccattg aaggaacttc gtatttcatt ttacggcttc
     3721 gccactcaac cttaacctca acctttttga atcatgaata acgaacaagg atttttgaat
     3781 tacgaagtga attgtgcaga ttgctccttt acgtatccgg tcaggttcac tgctcaccaa
     3841 tcactgttca ctgctcaccg aaggtgctta acctcaacct tgacctcacc actgacaatt
     3901 gaccactgat tactcacaac tgaccactga tattatgttt tcattcaaaa acgattacgc
     3961 tgaaggctgc caccccgaac tgctgaaaat gctcatcgac accaacatgt cgcaacagga
     4021 gggctatggc gacgacgatt tttccggact cgcccgcaca cgcattctgc aacaactgca
     4081 atgcgatgcc gacattcatt tcgtatcggg cggaacacag gccaacactg ttgtactggc
     4141 cgctgcgctg aaaccattcg aatcggtcat cgcagccgaa agtgcccaca tcaacgtgca
     4201 tgaagccggc gccatagaag ccaccggaca taagattgaa tacgcttcaa cgcccgatgg
     4261 aaagctcact ccggagctga ttgcaccgct gctcatcaag gccgaagatc atcacatggt
     4321 gaaaccacgt ctggtatata tttcacaatc gaccgagctg ggaaccgtgt ataaactggc
     4381 cgaaatagaa gctttatcgg cattctgcaa acaaaacaat ctgctgctgt atgccgacgg
     4441 cgcccgtttg ggtgcggctt tgacatcacc ggcagccgat ttcacgctgg ccgacatggc
     4501 gcggctgttc gatgcgtttt atatcggcgg caccaaaaac ggtgcactca tgggcgaagc
     4561 cattgttttg ctcaaccctg atctgaagcg cgatttcctg tttcatctca aacaacgcgg
     4621 tgcattactc tcaaaaggtc gcctggccgg atgtcagttt gcagctttat tcagcaataa
     4681 tctgtttttt gatctggccc gacattcaaa caacatggcg ctggaaatgg ctgctgctat
     4741 tgctaaagca ggttatccgt tttttgtaca gcccgaatcg aatcagattt ttccgattct
     4801 gccaatgaca ctgatcgaaa aattatcgaa agaattcggc ttttacatct ggcgtaaaac
     4861 cgatgaccaa cacgccatca tccgcctggt tacttcgtgg gcaacaccgc aaagcgaggt
     4921 ggagaggttt attgaaaatc tttaaatgaa caggttttat tcatactctc tgattgcaat
     4981 tgtgcttttc tcggcaaatg gcctgaaagt acatgctcag tatcccggct atttcaagct
     5041 gaaaacaata aatggtactg tttacaatac tgataatacc ccgattgatg ttgaatttat
     5101 ttcgcggtat aagcttaaaa tcaccaattg taccgatccc gatgttgaca cattactacg
     5161 gactctcact ttaacccgtg tgagcaggga ttacctgact catttaatta caacatctgc
     5221 aaaagttgtg attaatgtat ccgacaatat tggcattgcg ttctctgaag gaaaatacag
     5281 attcattgcc ggaatatgtg gacctgacaa agaccaaagc catacgttaa ttccggatct
     5341 gacaagcagt ttatgcccga gaaaaacgtt caggcatccg gtaaatgtgt atgcacaaag
     5401 tactataact ctgtttaaag gatctatctc gtatgtgcat actggcgttc ctgaactggc
     5461 tgaacaaaat gtgcaaattt tcgatcttga ttcaaacagg tgtataaaaa cattttccat
     5521 ggattcagtt gtcgttgaaa aaataatgta tcctgatatg ctttatcgga acacccggga
     5581 attatatttc tttggcggaa cacatgaagt ttgtcattct actgcagaga atatagcagt
     5641 caatgatagt gacctttgcg aagcgatggc atttgatatg gaaaaaaaac tattcaaaaa
     5701 acgaaaggcc attaatcgta aaaaaatcat atatccaaca aaataatcta tcctgatttc
     5761 ctattcattt tcgaatctca aatattcgaa agaaataaag aatctcctga ttcattcaat
     5821 cttcgctacc ggaaagcgat gttgagaaat gagtgccaca atgctgctga acaaatccga
     5881 atttttttat ccggtttatt ttctgtaact tagcccggaa attggaaaat gagggtgaat
     5941 catcatgctg acgaatgtcc ataccaaaag cctggatgaa ttgtacagaa cgcctattgt
     6001 acaacagact gcattctggt cggtcgtaaa acaaaagctt ggcgaaaaaa cactggcagt
     6061 gcattacagc tcgcgtgcat cggatttatt tggtcctgtt tcatatgaaa aatatattaa
     6121 ctccgattta ctggtcatca taaaacgggt catgcccggc tattccatgg cctatgttcc
     6181 gtatggtccc gaacttgaac ccagcgctga aaatcagggt gctttcctcg aagaactggc
     6241 cgaatgtatg cggtcgtttc ttccgaaaaa ctgcatcaac attcgttttg atttatgctg
     6301 ggaatcgtac tggagcgacg gtgagagctt gtcagagcct tcaattaatg ctcaggagct
     6361 gcggttcaac atcaatacca acaagtggaa tttcagaaaa tcaatgttca ataatctgcc
     6421 gtcaagcacc atgtttgttg atctgaagcc ggatacaaga aatattattg atttaatgaa
     6481 acccaaaact cggtataata tcggtctggc agagcgaaaa aatgtggtgg taaaacaaat
     6541 gggaatcgaa tcattgccgc tgtggtacca attgtacgag cagacggcaa tacgaaataa
     6601 tatttttctg aacgacatta aatattttga agctgtgttg actgctcaga ccaacgatgc
     6661 ccgttcgccc gcagaagtta tcctgctgat ggccgaatac gactcaatgc cgctggcagc
     6721 tatgttttta atcatcagcg gtaaccgtgg atcttattta tacggggctt cgtcatcgga
     6781 acatcgcaat aaaatggcaa cttatgcttt gcaatgggca gccatgaata tttcaaaaaa
     6841 gcatggctgc accgaatacg acatgtttgg cgtttctcca aatcccgatc ctgatcatcc
     6901 gctgaatggc ttgtataaat tcaaatcggg ctttggcggc gaactgtttc acaggcttgg
     6961 ttgctgggat tatccgctgt gtaatgacca atatcaaatt ctgaaatcca ttgaactgaa
     7021 tcaaatgggc tttcatctga attaatgaat gactacgggt gcgccacaaa aaaacaatta
     7081 tatttgatct ttgtgaatat ttcactttaa ggcatcctgt gaattctaat ttttaattca
     7141 tgaaacacta tagtctgttc ctgatttcat taatgttgtt ctacctgtca gcctgcacgc
     7201 aaaaaggaag ccagcagaat gacgattcaa atcaattatc ctacagcatt aagcgcaccg
     7261 attttgtcat gggtacacac aaaaattttg agtggattct gcaaaacgac agtgtgaaag
     7321 tgattgagta tccgatgagt tccgaccctg acagtatggt gtgctacaag gcgctgtcgg
     7381 agcggcagag aatcaagttg caaagcataa tgaaaaccat tgatctgaca cagatgcagg
     7441 atagatacag cgacgatctg gtacttggcg aaggacattc ggtgtacgat atttcaatca
     7501 acggatcctc gaaacacatc tatgtttatt atgttgatgt accagagctt attaaaattg
     7561 atgaattttt gtatgatgta actgctgatt gcaaataaaa attgtcatac tcttgctgtc
     7621 acactgagcc ctgcgttccg cgggataaac tccgtcgaaa tgtcggaagc gaaaatcaaa
     7681 aaaaaaagtc tgcagtataa gatgagatcc cagctctaca caagttttat gatgcccggg
     7741 aactgaattt tagttttggc cgggaacgca aaaccacagt ttttgttgat tttcaataat
     7801 cataaagaac gtttcatcaa cagcactcca tggcgctgat atgatatcca tgacattgaa
     7861 ataatcatca tcatttttat tcaatatctt tgcaaaaaac ataagcattg aattttacag
     7921 aatttagttt tacgccgcag ctgatggaag gcatcgatgc caacggatat gaagcggcaa
     7981 cacccataca ggaacaggcc ataccagcca ttttaagcgg acgtgatctg attgcgtgcg
     8041 cacagaccgg caccggaaaa acagccgcat ttctgcttcc gctgacgaat aacatcatta
     8101 cttcggaaca aaaccaccgc atcaaatgca tggtgattga acctacacgc gagctggcca
     8161 tacaggtcga tcagcagatg cagggctttt cgtatttcac cag
//
LOCUS       DAOPKE010000113         8176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9329_length_8176_cov_10.796946, whole genome shotgun sequence.
ACCESSION   DAOPKE010000113 DAOPKE010000000
VERSION     DAOPKE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9329_length_8176_cov_10.796946"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..3985
                     /locus_tag="PKY63_11275"
     CDS             83..3985
                     /locus_tag="PKY63_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01240.1"
                     /translation="MKNVIFTFVSMLMLVALIQTNKVQAQTPTLTYPAPLSEYSPGQA
                     IDFDVDLYSNGLIDQLCAVRYYIYKDDMTTPLATVTPYGTISYTVRGQGTNYVTNAIT
                     DGQGFIEVEALNDDLQAFTLGIFDNYCVDRDRPINIDMNFTTPGSYRFTVEIVSCSNN
                     GVSTGTTFTDINCGGLVHTDSVSDTCMTPVILNTYSIDFKVCGSAQITRLSGPTACIA
                     GEEIEIVYSLGNFDDAIDATQIPTWLTYAIDNINHTLTLSGTAPATNGNQTYNFVITT
                     QSTINPASCATANITENITVTAPQVVYSSVNSSYNTGDNITTSALVYSNGLVDELCGV
                     RYAIYKDNSTTPIDSVNDYGTVTYTVRGQGATMVTNPIKAGQGYIEVYALSDTLQAFT
                     LGIFDNFCLDRDRPIQIDMNFTIPGNYRFEAEIVSCGNAGISAGTTFTDINCGGTLHT
                     DSVAEECLNPVTLAQEEVETTICGEAEIAFVSGLTNYTPGDSVEFIYSLGDFDDAIDA
                     SQIPAWLTYSIDNATHTLTLEGVAPASDGVETSNFTIETASTINPASCPTASVVQNII
                     ITAPSVTYTPINVMYHTSENISTSALVYSNGMVDELCGVRYAIYKDNSTTPIDSVNDY
                     GTVTYTVRGQGTTLITTPLKTGEGYIEVIALNDTLEAFTLGIFDNFCINRNRPIDIDM
                     NFTVPGNYRFEAEIISCGNAGISAGTTFTDINCGGTLHTDSVAEECLNPIVLTEGEVE
                     TTICGEAAINYVSGDLNYFPGQPVEIIYSVGNFDDGIDNTNLPTWLGFIADTSNNTIT
                     LTGIAPAYDALTPSYSFALNTTSSTNPATCPTATTNLSIVVNEGPEIEFTSINAVYNV
                     GDSIYTTASIYSNGYVDELAAVEYDIYHNATLVNQVSDYGYMDYNVRVSGTNYDTTAI
                     ETGSGFITMSPVSDTLSAFTLGIFDNTCVNRNRPVNLFLTFDVPGEYVFNTELVSCAN
                     QGTATGTSFVATNCDNLIHYDSVAEDCLGPEMLFAETIGFTVCGESSIAYESGSDIYC
                     AGTEIDLVYNVGDFDDGLNTTNLPSWMTYSVNATNHTISLHGTVPEYDQLNPTYTITL
                     STTSSENPAACPNASMVQTITVKESYRDTITEVICEGDSVVVGTTAYYTEGIYPNTLT
                     AVNGCDSVVVLDLTVNPTVYSEFTVVTDSASYTWNSVVYDESGDYTQTLTSNITGCDS
                     IVTLHLTITIGIDELNNTILSVYPNPTDNFVYVETVAKTGSQCMIEVHDVYGRVISST
                     EITENKTMIDLSNLASGVYFLKVMDGEKITGTAFVTKQ"
     gene            4055..5905
                     /locus_tag="PKY63_11280"
     CDS             4055..5905
                     /locus_tag="PKY63_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01241.1"
                     /translation="MKKIYTILVLSFLIIPFFNIKSQAQIPTVQYTPLAPYYYTHNTI
                     YDTARVYSNGLIDQLCAIHYSIYKDDTLTPITSVDDYGSIEYTVRSYSTNYLTQSITN
                     GTGYLSVRPLFTTYNAFTLGIFDNYCVSRNRPVMLEMVFDEPGYYKFYCEIQSCSNSG
                     SSLGTSFTANGAPGCGTTSHSDYAATSCDNATPLFGEALYLTICEQGSIAYSSGNTTY
                     CPGEAISLVYTLGTFNDLADTTGLPSWLTPAVDVLAQTLTLTGTAPIYDPANPTCTFP
                     VSSKISVNPNGCPADFVSQTITIIDAPTPVIAGDNFICEGSSIELLSDLKGTWTSTDG
                     SIVTIDQTAPDSAVTITGLSDGVINIFCEATSGGCTVNSQPFSIIVHPVIKDTITTTI
                     CSNDQYLIGAVAYNTAGTFTDTLISSNGCDSIVTLHLSVNPVSDSALTETICQNNPFS
                     FGGEFINISGIYKDTLVNALGCDSIVTLNLTVNDTSFSEFWITDTSYTWNTIAYTESG
                     DYEQTLLNAYGCDSVVTLHLTVVTGIENNELNAEIYPNPAEDYIHIILNSKINNTQLK
                     LFNSEGILIKSYNMAGNKFSIELSSLAPGLYFLTISDSKGHSATYRFSKQ"
     gene            5945..7105
                     /locus_tag="PKY63_11285"
     CDS             5945..7105
                     /locus_tag="PKY63_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HPF01242.1"
                     /translation="MKPETTKERISSLDILRGFALLGIVVVNALGFNASFFNFGGYYG
                     ALPDPEQQEFYNVFISLTADKFIFLFSFLFGYGIFMQYKKFQDHNQPFGNFFSRRMMV
                     LAMFGIAHVVLLWAGDILLPYAIAGLIVFLLRKVPAVLQLMLAIFFYFFVAFWLAAST
                     YIPLPDSMTSLCTECIEQAGIIYSSGNYYEILQLRLHEYADFRNINLFYYLPKIIGLT
                     LFGFIASKNLLHKKVATRKATWSVVLALIAATAVFFYLKYETIFNIEGPFATAAYMAG
                     YELMNLLVASTYLLLILLIASFSTPARLLKPIALMGRMSLTNYLMQSLILGIIFYGWG
                     FGFFGQTDVMMVVAIALSVYLFQILLNIIYFRFFETGPLEKIWRKWSYGKLL"
     gene            7102..7848
                     /locus_tag="PKY63_11290"
     CDS             7102..7848
                     /locus_tag="PKY63_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01243.1"
                     /translation="MTFLIFEMKNTNIHFPFVLLLLVLISAGCSKEKRSNEAALKMQE
                     FVIDISQYARGIDPDFIVIPQNGPELAFKDAEPENELNQEYLAAIDGMGLEEIFYFET
                     YTPDEYRLDMLRKILPLKKIMVSEFVSSASLIPDALLKNYNESFICFVRTSDNYYYTK
                     IPDTIPFENSNNISTLADAQNYLYIINSENYSSKQDFLSAIDATNFDVILMDLFLEET
                     AFTATEINELKNKGQWRSAPGDCVYEYRLS"
     gene            7829..8080
                     /locus_tag="PKY63_11295"
     CDS             7829..8080
                     /locus_tag="PKY63_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPF01244.1"
                     /translation="MNIGSAENYRYYWQDDWKLHNPNWIKKKYEGYDDEFWVEFWNED
                     WQKIIFGNNDSYTKKIIDAGFDGAYLDNVEAYYFLYYKD"
BASE COUNT         2428 a         1860 c         1592 g         2296 t
ORIGIN      
        1 ccgcttatat ttgcatcgca tcactaagaa aaaaacaagt ttttagaggt gcccataata
       61 accaaaacac aaaaaacaaa aaatgaaaaa tgtaattttt acattcgttt caatgttgat
      121 gttagtcgcc ttaatacaaa caaacaaagt gcaggcccaa acaccaactc tcacctaccc
      181 cgctccatta tcggagtact cccccggaca agcaatcgat ttcgatgttg acttgtattc
      241 caatggtctg attgaccaat tatgcgcagt gcgttattac atttacaaag atgacatgac
      301 aacccccctt gcaaccgtga ccccctacgg aaccatcagc tataccgtcc gaggtcaggg
      361 aaccaattat gtaaccaatg ccatcactga tggacaaggt tttattgaag tagaagcatt
      421 gaatgatgat ttgcaggcat tcacccttgg tattttcgac aactactgcg tcgatcgtga
      481 tcgccccatc aatattgata tgaattttac gactcccggc agctaccgtt tcactgttga
      541 aatcgtttca tgttcaaata acggagtaag caccggaacg acatttacag atataaactg
      601 cggcggactt gtgcacacag attcagtttc agatacctgt atgacacccg ttatactcaa
      661 cacctacagc atcgatttca aagtctgtgg ttcagctcag ataacacgat tgagcggtcc
      721 taccgcatgc attgctggcg aagaaattga aatcgtttat tcacttggta atttcgacga
      781 cgccatcgat gcaacccaga ttccgacatg gctgacctat gcgattgata atatcaatca
      841 cacccttaca ctgagcggca cagctccagc aaccaatggc aatcagacat acaactttgt
      901 cattacaact cagtcaacca ttaatccagc ttcatgtgca acggccaata taactgaaaa
      961 cataacagtt acagccccgc aggttgttta ttcatctgtc aattcatctt ataatacggg
     1021 tgacaatatt accaccagcg ccttggttta ttccaatggc ctggtcgatg aactttgtgg
     1081 cgtgcgttat gctatttata aagacaactc aacaacaccc atcgattcag taaatgacta
     1141 cgggaccgtg acctacactg ttcgcggaca gggtgccaca atggttacaa accccatcaa
     1201 agccggacag ggatatattg aagtatatgc actgagcgac acactgcagg catttacgct
     1261 tggcattttt gacaacttct gccttgaccg tgatcgccca attcagattg acatgaactt
     1321 tactattccc ggcaactatc gtttcgaagc ggaaatcgtt tcatgcggca acgccggaat
     1381 cagcgccgga acaacattta cagacattaa ttgtggtggc accctgcaca cagattctgt
     1441 tgcagaagaa tgtctgaatc cggtaacact cgctcaggaa gaagttgaaa ccaccatttg
     1501 cggcgaagca gaaattgcct ttgtcagcgg actgaccaat tacacccctg gcgactcagt
     1561 agaattcatt tattcactgg gcgatttcga tgatgccatt gatgcatcac aaataccggc
     1621 atggcttacc tattccatcg acaacgccac tcatacactt actctggaag gcgttgcacc
     1681 agcttcggat ggcgttgaaa caagcaattt cacaatcgaa actgcatcaa ccatcaatcc
     1741 agccagctgc ccgacagcat ccgttgttca gaatattatc attacagctc cttcagtaac
     1801 ttacactcca attaatgtaa tgtatcacac atccgaaaat atttcaacct cggcactggt
     1861 atattcaaat ggaatggttg atgaactctg tggtgttcgt tatgctattt acaaagacaa
     1921 ctcaacaaca cccatcgatt cggtcaatga ttacggaaca gttacctaca ctgttcgggg
     1981 acaaggtact acattgatta caactccgct taaaaccggt gagggttaca ttgaagttat
     2041 tgcactcaat gatactttag aggcttttac gcttggaatt ttcgacaatt tctgcatcaa
     2101 ccgcaatcgt cctattgaca ttgacatgaa cttcaccgtt cccggcaact atcgtttcga
     2161 agctgagatc atttcatgcg gcaacgccgg aatcagcgcc ggaacaacat ttacagacat
     2221 taattgtggt ggcaccctgc acacagattc tgttgcagaa gaatgtctga atccaattgt
     2281 acttaccgaa ggtgaagttg aaacaaccat ttgcggcgaa gcagccatta actatgtaag
     2341 cggagatctg aattatttcc cgggacagcc ggttgagatt atttacagcg ttggcaattt
     2401 cgatgacgga attgataata ctaatctgcc cacatggctt ggctttattg ctgatacatc
     2461 aaacaacacc atcacattaa caggaattgc accggcatat gacgctctaa caccttcata
     2521 ttcctttgca ctgaatacaa catcaagcac taacccggct acttgcccga cagctacaac
     2581 caatctcagc attgttgtca acgaaggtcc ggagattgaa ttcacaagca tcaatgctgt
     2641 ttacaatgtt ggtgacagca tttatactac agcaagcatt tattccaatg gatatgttga
     2701 tgaactggca gccgtagaat atgacattta ccacaatgca acgctggtta atcaggtcag
     2761 cgattatggc tatatggatt acaatgttcg tgtcagcggt accaactatg atacaacggc
     2821 tattgaaaca ggttcgggat ttattacaat gtctccggta agtgacactt taagtgcctt
     2881 tacacttgga atttttgata acacctgtgt taaccggaat cgtcctgtaa acttattcct
     2941 gacatttgac gtgcccggtg aatatgtttt caacactgaa ctagtgtcat gtgccaatca
     3001 gggaacagcc acaggaacat cgtttgtagc tacaaactgc gacaatctga ttcattatga
     3061 ttcagtagca gaagactgtc tcggacctga aatgttgttt gccgaaacca ttggttttac
     3121 tgtttgcgga gaatcatcca ttgcttacga aagcggatcc gacatatatt gcgccggaac
     3181 tgaaatcgac cttgtgtaca atgttggcga cttcgacgat ggactcaata caacaaacct
     3241 tccttcatgg atgacctatt cggtcaacgc taccaatcat actatttcac ttcatggaac
     3301 tgttcctgaa tacgatcagc tcaatccaac ctataccata acactaagca caacttcaag
     3361 tgaaaatccg gctgcatgcc caaatgccag tatggttcaa accattacag taaaagaaag
     3421 ctatcgtgat accatcaccg aagttatctg cgaaggcgac agtgttgttg ttggaacaac
     3481 agcatattat actgaaggaa tttaccccaa cacattaacc gccgtgaatg gttgcgacag
     3541 cgttgtggtt cttgatctga cagtaaatcc aacagtttat agtgaattca ccgttgttac
     3601 tgacagtgct tcatacacat ggaattctgt tgtttatgac gaatcaggtg attacactca
     3661 aacactgacc tcaaacatta ccggatgtga cagtattgta actttacatc tcaccatcac
     3721 cattggaatt gatgagttga acaataccat cctttcagta tatccaaatc cgactgataa
     3781 ctttgtttat gtggaaacag ttgctaaaac cggcagccag tgcatgattg aagtacacga
     3841 tgtctatggc cgcgtaatca gttcaactga aattacagaa aacaaaacaa tgattgatct
     3901 ctctaacctc gcttcaggtg tatatttcct gaaagtaatg gatggagaaa aaattacagg
     3961 tactgcattt gtaacaaaac aataatgcag aaaaattttc agggggtacg gcattcatgc
     4021 gtactccctg attttttcat taaaaaaaag aagcatgaaa aaaatatata ccatacttgt
     4081 cctttcattc cttatcattc cgttttttaa tataaaaagc caggcacaaa ttcccacagt
     4141 acaatacact cctctggcgc cgtattatta cacccacaac actatttatg atacagcacg
     4201 ggtatattca aacgggctga ttgaccagct atgtgctatc cactattcca tctacaaaga
     4261 tgacacactc acacccatca catcagttga tgactatgga agcattgaat acactgttcg
     4321 aagctatagt acaaactatc tgactcagtc aatcacaaat ggcaccgggt atctgagtgt
     4381 aagaccgctg tttacgacct ataatgcttt cacactcggc atttttgata attactgcgt
     4441 ctcacgtaac cggcctgtga tgctggaaat ggtatttgac gagccgggat attataaatt
     4501 ttattgcgaa attcagtcct gttcaaactc cggaagctcg cttggaactt cctttacagc
     4561 aaatggggcg ccgggttgcg gaaccacaag ccactccgat tatgcggcta caagctgcga
     4621 caatgcaact ccgttatttg gcgaagcatt gtatctcacc atttgcgaac aaggctccat
     4681 cgcttattcc agcggcaata ccacgtattg tcccggagag gcaatttcac ttgtatatac
     4741 tcttggcaca ttcaatgatc tggctgatac aacaggtctt ccatcctggc tgacacctgc
     4801 cgtggatgta ttggctcaaa ccctcacgct gacaggcaca gcgccaatct acgatccagc
     4861 gaatccaacc tgtacttttc ccgtgtcgtc aaaaatatcc gtgaacccaa atggatgtcc
     4921 ggcggacttc gtttctcaga ccattaccat catcgacgcc ccgactccag tcatcgcagg
     4981 agacaacttt atttgtgaag gaagctccat cgaactgctt tccgatctga aaggaacatg
     5041 gaccagcacc gatggcagca ttgtaaccat tgaccagaca gcacccgatt cggctgttac
     5101 catcaccgga ttatccgatg gtgtaatcaa tattttttgc gaagcaacaa gcggaggatg
     5161 cactgtaaac agccagccat tttctattat tgtacatccg gtaataaaag atacgattac
     5221 aacaacaatc tgttcaaatg accagtatct gataggtgca gttgcttaca acacagccgg
     5281 aacctttact gacacactga tatcatcaaa cggttgcgac agtattgtaa ccttgcattt
     5341 atctgtcaat ccggtaagcg attccgcatt aaccgaaaca atctgccaaa acaatccgtt
     5401 ttcatttggt ggagagttca tcaacatcag cggtatttat aaagacacgc ttgtaaatgc
     5461 acttggttgc gacagtattg ttacgctgaa tctgactgta aacgacacct cgttttccga
     5521 attctggatt accgatacaa gctacacctg gaatacgata gcatatactg aaagcggaga
     5581 ttatgaacaa acacttctca atgcatatgg ctgcgacagt gttgttacgc tgcatctgac
     5641 ggttgtaacc ggaattgaaa ataatgaatt aaatgctgaa atatacccaa acccagccga
     5701 agactatatt catattattt tgaattcaaa aatcaacaac acgcaattga agttatttaa
     5761 ttcggaagga attctgatta aatcctataa tatggcaggc aataaattca gtatcgaact
     5821 ttcatctttg gctcctggct tgtatttcct gactataagc gactccaaag gacattctgc
     5881 aacgtatcgt ttcagtaaac aatagtcatt tatatttttc gtagatttgt tttttgcgaa
     5941 aaatatgaaa cctgaaacaa caaaggaaag aatcagcagt cttgatattc tgcgcggatt
     6001 tgccttgctt ggaattgtag ttgttaatgc actcgggttc aatgcatcct tcttcaattt
     6061 cggcggatac tacggggctt tacctgatcc ggaacagcag gaattctaca atgtgttcat
     6121 cagtctcacc gccgacaaat ttattttcct gttcagcttc ctgttcggat atgggatatt
     6181 catgcaatac aaaaaatttc aggaccacaa tcaaccgttc ggcaacttct tttcacgtcg
     6241 catgatggtt cttgcaatgt ttggaattgc gcatgtagtg ctgctttggg caggagacat
     6301 tctgttgcct tatgcgatag caggattgat tgtttttctg ctgcgaaaag tcccagccgt
     6361 tttgcaactg atgctggcca ttttctttta tttctttgtg gcattctggt tagctgcatc
     6421 aacttatatc cctcttcccg actccatgac atcgctctgc actgagtgta tcgagcaagc
     6481 cggaattatt tattccagcg gaaattatta tgaaattctt cagctgcgat tgcatgaata
     6541 tgcagatttt cgcaacatca atctgtttta ttatctgccg aaaatcatcg gtctcacctt
     6601 gtttggattt attgcctcaa aaaatttact tcataaaaaa gttgcaacac gcaaagccac
     6661 atggagtgtg gttcttgccc tcatcgcagc cacggcggtt ttcttttatc tgaaatacga
     6721 aaccattttt aacatcgaag gacctttcgc tacagctgct tacatggccg gttatgagct
     6781 gatgaattta ttagtcgctt cgacttattt actattaatt ctgcttattg catcgttcag
     6841 cacgccggca cggctactga aaccaattgc gctgatggga cgtatgtcgc tcaccaatta
     6901 cctgatgcaa tcgctcattc tcggaatcat tttctacggc tggggatttg gattcttcgg
     6961 tcaaaccgac gtgatgatgg ttgttgcaat cgcgctctca gtctatcttt tccagattct
     7021 cctgaatatt atttatttcc gtttcttcga aaccggaccg ctggaaaaga tttggagaaa
     7081 gtggtcgtat gggaaactct tatgacattt ttgattttcg aaatgaaaaa cactaatata
     7141 cattttccgt ttgttttatt gcttctggtg ctgatttcag ccggatgctc caaggaaaaa
     7201 agatcaaacg aagccgcatt gaaaatgcag gaatttgtta ttgacatctc tcaatatgcg
     7261 cgtggcattg atcctgattt tattgtcatt ccgcaaaacg gaccagaact ggctttcaaa
     7321 gatgctgaac ccgaaaatga attgaaccag gaatatctcg cggccatcga tggaatgggc
     7381 ttggaggaaa tattttattt cgaaacctat acgcccgatg aatacaggct ggatatgctg
     7441 aggaaaatat tgccattgaa aaaaatcatg gtatcggaat ttgtaagctc cgccagtctg
     7501 attcctgatg cactgctaaa gaattataat gaaagcttta tttgttttgt gcgaaccagc
     7561 gataattatt actacacaaa aattccggat acgattccgt ttgaaaacag caacaacata
     7621 agcaccctcg ccgatgcgca gaattatctg tacatcataa acagcgaaaa ttattcatcg
     7681 aagcaggatt ttttgagcgc catcgatgcc accaatttcg atgtcattct gatggattta
     7741 tttttggaag aaacagcttt tacagccact gaaatcaatg aactgaaaaa caaaggccaa
     7801 tggcgctcag cgcctggtga ttgcgtatat gaatataggc tcagctgaaa actaccgcta
     7861 ctactggcag gacgactgga aactgcataa tccgaactgg atcaaaaaaa aatacgaagg
     7921 atatgatgac gaattctggg tggaattctg gaatgaggac tggcaaaaaa ttattttcgg
     7981 caacaatgat tcatacacca aaaaaatcat cgatgccggc tttgatggcg cctatctgga
     8041 taatgtggag gcgtattatt ttttgtatta caaggattaa gaaataaata gggcctgctg
     8101 aaaaacactt aacactcaca gaattagaaa catattcatc aaattgggca ttgcaaatgc
     8161 aagtgtattt atcggg
//
LOCUS       DAOPKE010000114         8111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9410_length_8111_cov_10.315045, whole genome shotgun sequence.
ACCESSION   DAOPKE010000114 DAOPKE010000000
VERSION     DAOPKE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9410_length_8111_cov_10.315045"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..684
                     /gene="rpe"
                     /locus_tag="PKY63_11300"
     CDS             34..684
                     /gene="rpe"
                     /locus_tag="PKY63_11300"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPF01245.1"
                     /translation="MTLKIAPSILSADFLNLGKEIEMLNRSEADWIHVDVMDGVFVPN
                     ISFGFPVIEAVAAIAKKPLDVHLMITQPERYIEQFVKAGAQYLSIHYEGAIHLNRTIN
                     QIKELGAKAGVVLNPHTSVDVLKDIIADLDYVLLMSVNPGYGGQKFIPATLKKIEAMN
                     LMRRSRNQHCLIEVDGGVNEKNAQDIIEAGADVLVAGNAVFKSSDPEQAIKGLKNR"
     gene            complement(712..1158)
                     /locus_tag="PKY63_11305"
     CDS             complement(712..1158)
                     /locus_tag="PKY63_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01246.1"
                     /translation="MYETFYTGDSGVQYFIKPLNYSTDDKSVLLRIDFAFRYLDSNDC
                     PVITNISLINKSAFIMADSLSFSGIDNAVFKGNTPKLLFKEVRSKSYITRYSSEVRLN
                     KLTDFFKNGKSSVFLNSGANHFVFHPVKKTKRHIEKLNRNVFPCFE"
     gene            complement(1422..1703)
                     /locus_tag="PKY63_11310"
     CDS             complement(1422..1703)
                     /locus_tag="PKY63_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HPF01247.1"
                     /translation="MKRIRIFAVSIAVMLMMASCAVTLPVNATSNPVGSKVGTATATG
                     YLGILFFNQDASIKAAAKNGGITKISTVDMKQTSLLGIIVTYETIVTGE"
     gene            complement(2409..2780)
                     /locus_tag="PKY63_11315"
     CDS             complement(2409..2780)
                     /locus_tag="PKY63_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPF01248.1"
                     /translation="MKTDNKPTLVLGASPNPSRYSYLATLRLSEHGHTVYPLGVRDGK
                     IGDFDILNSKQNGVKVHTVTLYLGEKHQKEWEDYILSLNPERIIFNPGAENQGLFVKA
                     SQKGIHCLEACTLVMLASNTF"
     gene            3060..5654
                     /gene="clpB"
                     /locus_tag="PKY63_11320"
     CDS             3060..5654
                     /gene="clpB"
                     /locus_tag="PKY63_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPF01249.1"
                     /translation="MNLESFTIKSQEIIASAFQKAFENKNQAIEDVHILSTLLQQDEQ
                     VTPYLLKKLGANAVVMKQLSDKEIESRPKVDGGQPYLAPSGLKTIQQAMSKQKELSDE
                     FVSLEHILLGLVESDGVAERILKDAGVTEKDLKAAISDLRKGSKVTSAGAESQYNALN
                     RYARNLNDLALKGKLDPVIGRDEEIRRLLQILTRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIIKGDIPENLKDKVIFSLDMGALIAGAKFQGEFEERLKSVIKEVIDSDGQIILFID
                     EIHTLVGAGANQSAMDAANILKPALARGELRAVGATTLNEYQKYFEKDKALERRFQVV
                     MVDEPSPADSVSILRGLKERYETHHQVRIMDEAILAAVELSNRYITDRFLPDKAIDLI
                     DEAASKLRLEINSVPEELDELERRIRQLEIEREAIKREKDEQKLDQLNKEIGQLSDVR
                     NVLTAQWREEKEVVDKIQQTKKSIEEYKFEAEQAERSGDYGKVAELRYGKIKEAETLL
                     HELEDSIKNKKSGHALINEEVDAEQIAEIVSRWTGIPLNRMLQSEKEKLLHLEEELHK
                     RVIGQNAAIEAVSDAIRRSRSGLSDEKRPIGSFIFLGTTGVGKTELARALAEYMFNDE
                     NSMVRIDMSEYQERHSVSRLIGAPPGYVGYDESGQLTEAVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDEGRLTDNKGRTANFRNTIIIMTSNLGAEIIRDNYEDLKPGSESQVFR
                     KTREEVFDLLRHTLRPEFLNRVDEIIMFKPLLPEEIKEVVKLQLKQLIEMLESQDIKL
                     EYTEFALDELANEGYDPQLGARPIKRLIQRKIINELSKQLLAGNISKEHGVKLDVIEG
                     RYVFLQMQ"
     gene            5780..6262
                     /locus_tag="PKY63_11325"
     CDS             5780..6262
                     /locus_tag="PKY63_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01250.1"
                     /translation="MKKLFVLFIFIAGIGLQSNAQVNPHAIGLRLSGGDDLVGGEVSY
                     QHGLGDANRLEFDLGWSGNDHHNRMYITGIYHWDWNLTGGLNWYVGPGAGIGLYHYDY
                     DDDYVNIAVGGQLGLEYDFNKNGAPILLSIDIRPMWDFLGDDHAGLGWGAALGIRYTF
                     "
     gene            6335..6826
                     /locus_tag="PKY63_11330"
     CDS             6335..6826
                     /locus_tag="PKY63_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPF01251.1"
                     /translation="MFNSLFSCRSIPQGVDAVQNFNADKYLGQWYEIARFDFRFERNM
                     NNTTANYSKNADGTIKVVNRGYNYVTNEWSEAIGKAKFIKTPDIAMLKVSFFGPFYGG
                     YNVIALDAEYKYALISGQNKNYLWILSREKSIPENIRTEYVNKAQNLGYDTSKLIWVK
                     QDA"
     gene            6990..7910
                     /locus_tag="PKY63_11335"
     CDS             6990..7910
                     /locus_tag="PKY63_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01252.1"
                     /translation="MRSFGVFLLMFLLSSLYPALSQENSEIFIGATSEEYSIRADVEL
                     EFVEKVFCESTKDTLFRKLDFSSPLFIQFNKADLENFLKHPEVFADHVDPFISTTYVY
                     SDHLGNNEIVPGLMKTHDESFADNQTESWTRDVSGERGTSLFIGFSILNFKESLEKTE
                     KPTFTLLFKLDPCTSCIEKNVSGKAVFKSIYNPTITVTEGLSLDAEFSHEYLFSPALA
                     IAVTDAGLTETTGEEREQFEENFKKQFYATAPKIDAEQLIRFLLHPSGTLEIPFEGFI
                     YSNAVCRQNNTFKGVIKVFGNQVYRFMPAE"
BASE COUNT         2560 a         1632 c         1653 g         2266 t
ORIGIN      
        1 catattgctt tttattatct ttgtaaaaaa aatatgacgc tcaagattgc accatcgatt
       61 ctttccgccg attttctgaa tctcggaaaa gaaattgaaa tgctcaaccg cagcgaagcc
      121 gactggattc acgtagatgt gatggacggc gtttttgtac ccaatatttc tttcggtttt
      181 ccggtgattg aagcggtggc ggccattgca aaaaaacctc tcgatgtgca tctgatgatc
      241 acacaaccgg aacgatatat tgaacaattt gtaaaagcag gcgcacaata tctgagcatt
      301 cattatgaag gcgccattca tctgaaccgc accattaatc agattaaaga actgggtgcc
      361 aaagccggcg tggtactcaa tccgcataca agtgtcgatg tgcttaaaga tatcattgct
      421 gatcttgatt acgttttgct catgtcggtc aatcccggct acggcggaca aaaattcatt
      481 cctgccacac tcaaaaaaat cgaagcaatg aatttaatgc gtcgctccag aaatcagcat
      541 tgtctgattg aagttgatgg tggtgtgaac gaaaaaaatg cgcaggatat catcgaggcc
      601 ggcgccgatg tgttggttgc aggcaatgcc gtttttaaat cgtccgatcc ggaacaagcc
      661 attaaaggcc tgaaaaaccg ataaagagat acatatcttt aaacaatagt tttattcaaa
      721 acatgggaaa acattccggt ttagcttttc aatatgtcgt tttgttttct taacaggatg
      781 aaatacaaaa tggttagcgc cagaattgag aaaaacagaa gactttccgt ttttgaaaaa
      841 gtctgtcaac ttatttaatc tcacttctga agaatatctg gtgatataac ttttagatct
      901 cacttcttta aaaagcaact tgggggtatt tcccttaaac acggcattat caataccaga
      961 aaagctcaaa ctatcagcca tgataaatgc tgatttgtta atcaaactaa tgttcgtaat
     1021 tacagggcaa tcattactgt ccaaataacg aaatgcaaaa tcaatgcgga gtaaaactga
     1081 tttatcatcc gtagaataat tcaacggctt tataaaatat tgaacaccac tgtcgcccgt
     1141 gtaaaaagtc tcatacaaat ttttaccgga tttttgcgct ccgggtctga tggaaataca
     1201 cgatgaaaaa aagagaatca tcaaaacaaa cccaatagca atgctgcttt tattggattt
     1261 tgcattaatg ataaaatacc tattcataaa atacctgatt tgagtaattt acacaaaaat
     1321 gccaaaaata tttcttatta atcatattca acaaatttat taaaaaataa atactgtcaa
     1381 atgccatttc aaagaaatat ttctggcatc tgcaaacgga attattcgcc agttacaatt
     1441 gtttcataag taacgataat acctaacaga ctggtctgtt tcatgtcaac tgttgaaatt
     1501 tttgtaattc cgccattctt ggctgcagct ttaatactgg catcctgatt gaaaaacaag
     1561 attccaagat atccggttgc tgttgcagtt ccaacttttg aacctaccgg attactggtt
     1621 gcattcacag gaagagttac ggcacaacta gccatcatta acattacagc aattgaaact
     1681 gcgaaaattc taattctttt catcatggtt taatttaaat aatccctact ctataggctt
     1741 ttcgggtaac gccattttaa aaattgttat aacatgcaaa tataattatt tcacaattaa
     1801 aattggctta aaacacatcc tgctgaaatt ttcttaaata ttatatgttc atttttctta
     1861 atgacagtat tcacccatgt caatcatatc agaatgatcc tcatgttttt aaagcggctt
     1921 tggccaatgt gattttcaca aactgcattt tttcgaacat ggatccgccg caaatgaagt
     1981 agaatgttgt ttttgcacac gatttccttt tgagacaacc tccgataaaa gccgaagcgt
     2041 ttttctggaa ttattgcaaa agctcaataa tatttattga ctgtctaaaa atgtactttg
     2101 ttacgacatt ccaccgttca ggcatacaat ttagctgata aaaagctatg acgcctgaac
     2161 atggaaccgt cgtaacaaat tcaaaaacaa cggttccatg tcctttcgct ccttcgtcgc
     2221 ttcagggagt ttatcccgcc gcggcgggag tggaatgttg tttttgtcac ataaataaat
     2281 tttagacagt ttcttaaaat gatttcatat aaaaacaaca atgttattgg tgttttaaat
     2341 ttctacattg ctgtatttat atgtgttgca tattttccac caatcacagc aatcaatctc
     2401 aaaaagaact aaaaagtatt actggccagc atcaccaaag tgcaggcctc gagacaatga
     2461 attcctttct gactggcttt gacaaacaat ccttggtttt cggctccggg attaaaaata
     2521 atacgctccg gattcaatga aagaatataa tcttcccatt ctttctgatg tttttcgcca
     2581 agatacaagg ttactgtatg aacttttaca ccattttgtt ttgaattcaa tatgtcaaag
     2641 tctccgattt tcccatcacg aacccccaac ggataaactg tatgcccatg ctctgaaagt
     2701 cgtaaggtgg ccagatatga ataccttgac ggattcgggc ttgccccgag aacgagtgta
     2761 ggtttattat cggttttcat aacagtttct gtttttattt tctgcaatac gaatatttga
     2821 tgctattatt ctgacaaggt ttaatatttg ttttaagcaa actattgcaa attcatactg
     2881 ctgattatct gcatattaga aagatttcta cacgatcaat accttaacat taacaaatat
     2941 tcaggtttta cataacaggg cattatgaca taaatacctc attaaaatca gtcaaattgt
     3001 cattatatcg actgttttgc atgatggaga gaaatttgat atttcctgaa aaaaaaatta
     3061 tgaatctcga atcatttacc ataaaaagtc aggaaatcat tgcttcggcc tttcaaaaag
     3121 cttttgaaaa caaaaaccag gcaattgaag acgtgcacat attaagtacg ttactacaac
     3181 aggatgaaca agttacgcca tatctcttaa aaaagctggg tgccaacgct gttgttatga
     3241 aacaactatc tgataaggaa atagaatcgc gcccaaaagt cgatggcggt cagccctatc
     3301 tggctccttc aggcctgaaa accattcagc aagccatgtc caagcagaag gaactgagcg
     3361 acgaatttgt cagtctcgaa catattctgc ttggactggt tgaaagcgat ggcgttgcag
     3421 agcgcattct taaagatgca ggtgtgaccg aaaaagatct caaagcagcc atttccgatt
     3481 tgcgcaaagg aagtaaagtt accagcgccg gcgctgaaag tcagtacaac gcattgaacc
     3541 gctatgcgcg caatctgaac gatctggctc tgaaaggcaa attggacccg gttatcggtc
     3601 gcgatgaaga aatacgccga ttgctgcaga ttcttacacg acgcaccaaa aacaatccta
     3661 tcctgattgg cgaaccaggg gttggaaaaa cagccattgc tgaaggtctg gcacacagaa
     3721 tcattaaggg cgatatacct gaaaacctga aagacaaggt gatattttca ctcgacatgg
     3781 gagcacttat agccggagcc aagtttcagg gcgagttcga agaacgacta aaaagtgtaa
     3841 tcaaggaagt gattgacagc gacggacaaa tcattttgtt tattgacgaa attcatacgc
     3901 tggttggagc cggtgccaat caaagcgcca tggatgcggc caacattctg aaacccgctt
     3961 tggcgcgcgg tgaacttcgt gccgttggag caactacatt gaatgaatac cagaaatatt
     4021 tcgaaaaaga taaagcgctc gaacgccgtt ttcaggttgt gatggttgat gaaccctctc
     4081 ctgctgattc ggtcagtatt ttacgcggat tgaaagaacg ctacgagacc catcatcagg
     4141 tgcgtatcat ggatgaggca attcttgctg cagttgagtt atcgaaccga tacatcaccg
     4201 accgcttcct gcctgataaa gccatagacc tgattgatga agctgccagt aaactacgtc
     4261 tggaaattaa ctccgttccc gaagaactcg atgagctgga acgcaggatc cggcagctcg
     4321 aaattgaacg tgaagccatc aaacgagaaa aagacgaaca gaaattagat cagctgaata
     4381 aggaaatcgg ccagctgagc gacgttcgca acgtgctcac cgcacaatgg cgcgaagaaa
     4441 aagaagttgt tgataaaatc cagcagacaa aaaaatcgat tgaggaatat aaattcgaag
     4501 ctgagcaggc cgaacgctcc ggcgattacg gcaaggttgc cgagctgcgt tatggcaaaa
     4561 ttaaagaagc tgaaacactg cttcatgagt tggaagattc cattaaaaac aaaaaatcag
     4621 gacatgcact catcaacgaa gaagtggatg cagagcagat tgcagaaatt gtttcacgat
     4681 ggacgggcat tcctttgaac cggatgctgc aaagcgaaaa ggaaaaattg ctgcaccttg
     4741 aagaagagtt gcacaaacgg gtgataggtc agaatgcagc catagaagcc gtcagtgatg
     4801 ccatccgccg tagccgcagc ggactcagcg atgaaaaaag accgatcggt tcatttattt
     4861 ttcttggcac tactggtgtt gggaaaacag aactggcgcg ggctttagcc gagtacatgt
     4921 tcaacgacga aaacagcatg gtgcgtatcg acatgagcga atatcaggag cgacacagcg
     4981 tttcgcgact cattggcgcg cctccgggtt atgtaggcta tgacgaaagc ggccagctga
     5041 ccgaagctgt gcgtcgtaag ccatacagtg tcgttctgct tgatgaaatc gaaaaagccc
     5101 atccggatgt attcaatatt ttgcttcagg tgctcgatga aggacgtctc actgacaata
     5161 agggacgcac cgccaatttc agaaatacca tcatcatcat gacaagtaat ctcggtgctg
     5221 aaataatccg cgataattac gaggatctga agccgggaag cgaatcgcag gtgttcagaa
     5281 aaacgcgcga ggaagtattt gatttgctca ggcacacatt acgtcccgaa tttctgaacc
     5341 gcgtggatga aatcatcatg ttcaagccat tgttgccgga agaaattaaa gaggttgtga
     5401 agttacagct gaaacagctc attgagatgc ttgaatctca ggatataaaa cttgaataca
     5461 cagaatttgc actcgatgaa cttgcaaacg aaggctacga cccgcagttg ggcgcacgtc
     5521 cgatcaagcg gctgattcag cgtaaaatca tcaacgagct ttcgaaacag ttgctggcag
     5581 gcaatatttc aaaagagcat ggagtaaagc tggatgtgat tgagggccgc tatgtgtttc
     5641 tgcaaatgca atgataatca tcagccggaa atgttgcaat ctggttgcag gaattgtaac
     5701 tttgtattca ggcattaaag tttgtctttg atgaatgaac ataatgccaa atgattttta
     5761 ccattaaaaa atttcagata tgaaaaagct ttttgtttta tttattttca ttgcaggcat
     5821 tggtttgcaa tcgaatgcac aggtaaaccc gcatgcaatt ggtcttcgcc tcagcggtgg
     5881 agatgacctg gttggtggtg aagtttccta tcagcacggt ctgggcgatg ccaaccggct
     5941 tgagttcgat cttggctgga gcgggaatga tcaccacaac cgcatgtata ttacaggaat
     6001 ttaccactgg gactggaatc tgacaggcgg tctcaactgg tatgttggtc ctggagccgg
     6061 cattggattg tatcattacg actatgatga tgattacgtc aatatcgctg ttggcggtca
     6121 attgggattg gaatacgatt tcaataaaaa cggcgcaccg attctgttga gcatcgatat
     6181 ccgccccatg tgggattttc tcggtgatga ccacgccggt ttaggatggg gtgctgcact
     6241 tggaatccgc tatactttct gattattttt ttgacaaaca gatcccgaaa tgagaaactc
     6301 tttcagattt ttcttattaa taatttcaat tacaatgttc aattctcttt tttcctgccg
     6361 atcgatcccg caaggagtcg atgctgtaca aaatttcaat gccgataaat atctcggtca
     6421 atggtacgaa atcgctcgtt ttgattttcg cttcgaacga aacatgaaca atacaacagc
     6481 gaattacagc aaaaatgcgg atggaaccat caaggtcgta aaccgtggat acaattacgt
     6541 tacaaacgaa tggagcgaag ccatcggcaa agcaaagttt ataaaaacgc cagatattgc
     6601 tatgctcaag gtttctttct tcggtccttt ctatggtggc tacaacgtga ttgcacttga
     6661 tgcggaatac aaatatgcat tgatttcggg acaaaacaaa aactatctgt ggattctttc
     6721 acgtgagaaa tccattccgg aaaacattcg caccgaatac gtaaacaagg ctcagaattt
     6781 gggctacgac acatcgaagc tgatttgggt gaagcaagat gcttgatcac cggatcttta
     6841 aaaaaaaata attccgacac aaaaaaataa gcgaaaccca caacttatat tctacttttg
     6901 caggctgtct gaaaacagcc tgcaacttca atttcagaca gactttgatt tcaagtggca
     6961 tataatacgt atattcgtaa aaaaatcaca tgagatcttt tggtgtattt ttattgatgt
     7021 ttttgctatc ctcactttat ccggcattat cacaggaaaa ttccgaaatt tttattggcg
     7081 ctaccagcga ggaatattct attcgcgccg atgtagaact ggaatttgtt gaaaaagtat
     7141 tttgtgagtc cacaaaagat actcttttca gaaagcttga tttttcatca ccgcttttca
     7201 ttcagtttaa caaggccgat ttagagaatt ttttaaaaca tccggaggta tttgctgatc
     7261 atgtcgatcc gttcatcagc acaacctatg tatactcaga ccatctgggc aacaatgaaa
     7321 ttgtgccggg attaatgaaa acgcacgacg agtcatttgc cgacaatcag actgaaagct
     7381 ggacacgtga tgtttcagga gaaagaggaa catcattgtt tattggattc tctattctga
     7441 attttaaaga atcattggaa aaaactgaaa aaccgacttt cacactcctt ttcaagctgg
     7501 atccttgtac aagttgtatc gaaaaaaatg tttcaggaaa agcagttttt aaatcaatct
     7561 acaaccccac aatcactgta acagaaggcc tgtcgttaga tgctgaattt tcgcacgaat
     7621 atcttttcag tccggctctg gcaatagctg ttacagatgc cggcctgact gaaacaaccg
     7681 gtgaagaaag agaacaattc gaagaaaatt tcaaaaaaca attttacgcc actgcaccaa
     7741 aaattgatgc agaacagctt atccgctttt tgttacaccc atccggaaca cttgaaattc
     7801 cttttgaggg atttatttac agcaatgctg tttgtcgtca gaacaacaca ttcaagggtg
     7861 taattaaggt tttcggaaat caagtatacc gcttcatgcc tgcagaataa agagatccca
     7921 aaaaagattt tcatttttcg aaacaaaatg aaaaaagaaa ttttactttt acacgatata
     7981 agtatgaatc atgaaccgtg taaaaagcgc agccatagtt gtcggtgatc cgaaaatcat
     8041 ccggggctgg gtgatatacg actgggccaa ttcagtattt cagctcacca ttctttcagc
     8101 catatttccg a
//
LOCUS       DAOPKE010000115         8070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9470_length_8070_cov_11.288584, whole genome shotgun sequence.
ACCESSION   DAOPKE010000115 DAOPKE010000000
VERSION     DAOPKE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_8070_cov_11.288584"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKY63_11340"
     CDS             complement(<1..193)
                     /locus_tag="PKY63_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01253.1"
                     /translation="MTIQEYISAVNKLYKAGIATEHSYRGDLKILLESLVSGITVTNE
                     PSHIACGAPDYIITRKNIPI"
     gene            960..2792
                     /locus_tag="PKY63_11345"
     CDS             960..2792
                     /locus_tag="PKY63_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPF01254.1"
                     /translation="MKKQIYSILIVALLIISSTPILAQTAFKSMGMVSGTIQNDTVWA
                     SDTIHVTTNLNIPDSVTLTINPGTVVLLDDVCINAYGRIIALGAPGDSIIFKAADTTG
                     FSAGTMAGWNGITFDITQSTDTSRFSHCVFEYFSGTGDQSAIYTNGYQHLVVENSRFS
                     YNYSGDDASCLHLAYGTSAVVRHNEFFGNESYYSCLHVGCGSSGDISNPLIIENYFHH
                     NLGQDEGSSLKLSTHTTAHVINNVFVFNESYNGGAIVISGYGSPKLIGNLIVANHATN
                     NGGAIAVKYETSPDFYNNTIAFNTADNDGGAFSISCDATNITYQNNIIWGNSADEGDQ
                     FYYNSGGTTHEFLNNIIDGGLDSMYLSSGAFAGLFMNNSTEDPLFTDPMVGDFTINCA
                     SPALNAGSIPGIPMPLTDLNGAPRLNGPAYDLGAFEYLSAAIIVSNPQNVSIAAGNNI
                     TFAVSATGATAYQWQVSEDLGTTWVNVTNDATYTGAQTNTLGVFTDYPMNGNMYRCQL
                     EGACSGVYIFSDPAFLFVNYNVGIDETDAIIGVFPNPAKDVVNVANAEGKIFSMIDLN
                     GREVFKVQISQNVESLNISSLESGIYIIRITDESSVQNLRFVKE"
     gene            complement(2883..>3289)
                     /locus_tag="PKY63_11350"
     CDS             complement(2883..>3289)
                     /locus_tag="PKY63_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01255.1"
                     /translation="NLRVIANLYVRDGKFNEAIQLLKTVADVTVIDPADYNSIGEIFG
                     RNLNQLDSAELYFEKSLERYSEYAPALENMGLVQGMRGNYQQSLEYFLQAYPLDSSNA
                     DLLHNLSRTYRNLGDILKAEEMQVRADHLSNQ"
     assembly_gap    3290..3389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3390..4360)
                     /locus_tag="PKY63_11355"
     CDS             complement(<3390..4360)
                     /locus_tag="PKY63_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01256.1"
                     /translation="MKKNSAALKKPLQKHISSDSKPFRQQSFSQNYRLQALVIFLFAF
                     ALYANTLGHQYALDDDLMIRDNKFTKQGFSGIGKILTTDALAGVFGEQNLLQGGRYRP
                     LSQVFFAVEYELFGLNPFIGHLINVFLYGLLCVLLFHVLRKIFKNYTSSKWYFGLPFL
                     VTALFAVHPLHTEVVANIKGRDEIMSLIGSLGALWFALKYCENRKPLNLLWIAVIFIF
                     ALLSKENAATFIAVIPLTMYFFSDPKRKDYLVITAVLFSAFAVYALIRYNALGFFMNS
                     YEETELFNNPFAEASISQKYATIVYTWLRYLGLIVFAYPLTHDYYPY"
     gene            complement(4502..5254)
                     /locus_tag="PKY63_11360"
     CDS             complement(4502..5254)
                     /locus_tag="PKY63_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01257.1"
                     /translation="MKKTLIFSLLIVLGTGVFAQVHGFLGSRFFVSANFSLTPDLKKN
                     PLNNSHDNVFTIRTPISGDVNFVAGNGVSLGARATFTNVTGTFYRARYNDYFYYSGIA
                     NYKTQIFSLYLEGHSKNAYSVIDNYFRFGVCLASLKNSEYLYTDVESNYSVPVSNDFP
                     DSVQSLALNQSTAMSGFYYEFGNRVPLSDHLLFYYSLSGYLFPIRNVWYKDDVYPNIT
                     IRESNTYIRDLGKRRVGNTNMLNLNFGLVWAF"
     gene            complement(5251..7440)
                     /locus_tag="PKY63_11365"
     CDS             complement(5251..7440)
                     /locus_tag="PKY63_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPF01258.1"
                     /translation="MLRTVLFFVSWLTALLLTAQDFNQYQPIRCSGELPAEFYSDEFL
                     VTTADVGSIDPNASYKDIKSKKEFIRYSDFYLRYLLRSGDLMFGDPLTNYVNKVADIL
                     LNSNPELREKFRFYVSRYPEVNAACLPNGIVIVNIGLLAQLENEAQLAFVLAHEIVHY
                     VKKHGIDSYVEQNRIRRSVGEYRNVRKSDRLFAMLQYQKDLEFMADELGFRDYFSASG
                     YKLSAAESVCDVLLYSDFPVDEIPVSRAMFEDDWFHIPDKCWLDTVALISAIEDFDDE
                     KLTHPNIKKRRERLKNLLSEFSDNGNYFILPQKDFQNMREIARYEMVQLYLTDRKYVD
                     ALYLIHVLQQNHPESVYLNSAKAKAYYGLMRLKNEKSKSTAIRASTKIKGESQRMFHM
                     FKNLSNKELVTVCAREIWKAHLLNPADTGILDMASEIMIDLLNNCKIEDNYFRFKAPV
                     PVDSSAQMTTLDSSASKYDKIKQNQKKALQQDNFTYAFLSFADDKEFRRLYNDAEGIT
                     LVEEEGDYKKRKKSYEYNDSLFVGTSKVLFFDPGYLRITESKNSGERFLAVSKYEKQY
                     LAMVQDASKMMGVDLTVLDVSTMNEADEDRYNSFQLIQSWINELPEDDEEGEDFISAN
                     QDKLNQLAMEEGTRYIAFSGIVSMRSKHAFNLLSYYLLIFPPAYPLMIAYYLSPQYFT
                     LYYFYLYDITTGRRVYSESSLVKSRDYKYTVKQMVYSSMQRIKTRQK"
BASE COUNT         2523 a         1681 c         1560 g         2206 t
ORIGIN      
        1 caatcggaat attttttcgg gttataatgt aatccggcgc accgcaggca atatgagatg
       61 gttcattggt gaccgtaatt ccggaaacaa ggctttccaa aagaattttc aaatcacctc
      121 tgtagctgtg ttcggtagcg atgccggctt tatagagttt gtttacggct gaaatgtatt
      181 cttgaatggt catgaaatat gcattaattc cccaaaacta atgattttct gttaatattg
      241 attgctatgt gtcgaaaata aaaatcgaaa acccggcaga agtgcattcg gtgccgggtt
      301 ttacaagcgg gccactgccc cgcaaaacga gttctcagaa gtaaaaaagc gaaatcacag
      361 gataaatatt tcagtttaat ctgctgttga caatctttgc agaaaaggca tgcaatcata
      421 aatactattt ttcagcaaaa aactttttga ttcacataat ctcaacagct acttagggtc
      481 tgctgaaaaa gtctgaggtg cgccataccg taacttcgta cggcacaagt attcgaagcg
      541 acaagacgaa gatgtatagt aatacttcga atcgcaggca acgaagaaga tggcgctcat
      601 cagactaata ccgtgcatat gaaataagca ttgaaacggc acaagtaaac actcacagca
      661 atagttgagg ttgttttgaa atgatgggca aaaaatactt gatgattgta tgatttcaaa
      721 aattacagca gcggattctc aaatgcagtg ctaattctct aaccgaataa aaacacttgc
      781 atttgcaacg tccaatatga tgaatatgtt tcagatatta tgagtgttac gtgtttttca
      841 gcaggaaata gttaaaaaac aacagcttat actataaatt atttacacca ataataatat
      901 tttaaagtta agcattgaaa taagccatgg aatcactatc tttgcgaaaa acaaatccta
      961 tgaaaaaaca aatctactct attctgatcg ttgcattact gattattagc agtacaccga
     1021 tccttgcaca aactgcattc aaatccatgg gcatggtgtc cggcaccatt caaaacgaca
     1081 ctgtatgggc aagtgataca atacatgtaa ccaccaacct gaacataccg gacagcgtta
     1141 cattaaccat taaccctgga acagttgttc tgttagacga tgtatgtatt aatgcctacg
     1201 gcaggatcat agccctggga gcccccggcg attcaatcat ttttaaagct gccgacacca
     1261 ccgggttttc ggcaggcacc atggctggat ggaatggaat cacgtttgat ataactcaat
     1321 cgaccgacac ttcccgtttt tcgcattgtg tttttgaata tttttcaggt actggcgatc
     1381 aatcagcaat ttacacaaac ggatatcagc acctcgttgt cgaaaacagt cgtttttcat
     1441 acaattattc aggcgacgat gcatcctgtc tgcacctggc ctatggtacc agtgcagtcg
     1501 taagacacaa tgagtttttc ggaaatgaat catattattc atgtcttcat gttggttgtg
     1561 gtagttcagg tgacatcagc aatcctttga ttattgaaaa ttatttccac cacaacctcg
     1621 gacaggacga aggttcaagt ttgaagctct cgacacatac aacggcacat gtgataaaca
     1681 atgtctttgt gtttaatgaa agttacaatg gcggagccat agttatcagc ggatacggca
     1741 gcccgaaact tatcgggaac ctgattgttg caaatcacgc caccaacaat ggaggtgcca
     1801 ttgcagtgaa atatgagacc agccctgatt tctacaacaa cacaattgct tttaatacag
     1861 ccgacaatga tggaggtgct ttttccatca gttgcgatgc caccaatatt acttatcaga
     1921 acaacattat ctggggaaat tcagcagatg agggcgatca gttttattac aattcaggtg
     1981 gaacaacaca tgaattcctg aacaatatta ttgatggcgg acttgacagc atgtatttaa
     2041 gttcaggtgc tttcgccgga ttattcatga ataattcaac cgaggatcct cttttcacag
     2101 acccaatggt tggagatttc actatcaatt gcgcatcacc agccctgaac gccggctcaa
     2161 ttccaggcat tccaatgcca ctgaccgacc tgaacggggc tccccgcctc aatggaccgg
     2221 cctatgatct tggtgctttt gagtatctct cagcagcaat aattgtctct aacccgcaaa
     2281 acgtatcaat cgctgcagga aacaatatta cattcgccgt ttcagcaacc ggagcaacag
     2341 cttaccaatg gcaggtcagc gaagatcttg gaaccacctg ggtgaatgtt accaacgacg
     2401 ccacttatac cggagctcag acaaatacac tgggtgtttt caccgattat ccgatgaacg
     2461 gaaacatgta tcgctgtcag cttgaaggtg cttgtagcgg tgtttatatt ttcagcgatc
     2521 ctgcatttct gtttgttaat tacaatgtcg gtattgatga aacagatgcc attatcggcg
     2581 ttttcccgaa tccggcaaaa gatgttgtca atgttgcaaa tgcagaaggc aaaatattct
     2641 ccatgattga tctgaacgga cgtgaagttt tcaaagttca aatcagccaa aatgttgaat
     2701 ccttgaatat ttcttcactg gaatcaggaa tttatattat tcgcattacc gatgagagca
     2761 gtgttcagaa cctgcgcttt gtaaaagaat aatttcttga ttttttgtac gaaaaattaa
     2821 aacggggatt catcagagtc cccgttttgt tttgcaattc aattacccat tatttctcaa
     2881 tttcattgat tgctgaggtg atctgccctg acctgcattt cttctgcttt caaaatatct
     2941 ccaaggttcc ggtaggttct gctcagatta tgcaacaaat cagcgttgct tgaatcgagt
     3001 ggataggcct gtaaaaaata ttccagcgat tgctgataat tgccacgcat tccctgcacc
     3061 aatcccatgt tttcgagtgc cggcgcatat tcagaatatc tttcaagcga cttttcaaaa
     3121 tacaattcag cactgtcaag ctgattcaga ttgcggccga aaatttctcc gattgaattg
     3181 taatcggcag gatcgataac cgtaacatcc gcaacggtct tcaatagctg aatagcttca
     3241 ttgaattttc catcccttac ataaagattg gcaattacgc gcaaattggn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gataaggata ataatcatgc gtaagtggat
     3421 aagcaaaaac aatcagtccg agatagcgca accacgtata aacaattgtt gcatatttct
     3481 gcgaaatact ggcttcagca aaaggattat tgaatagttc ggtttcttcg taactattca
     3541 taaaaaagcc cagcgcgttg taacgtatca gcgcataaac ggcaaatgct gaaaaaagaa
     3601 cagccgtaat tacaagataa tctttgcgtt ttggatcgct gaaaaagtac atggtgagcg
     3661 gaattacagc aataaaagtt gctgcatttt cttttgagag caaagcaaaa ataaaaatga
     3721 ctgcaatcca caaaagattc aacggctttc tgttttcgca gtatttaagc gcaaaccaga
     3781 gtgctccgag cgaaccgatg agactcatga tttcgtcgcg gcccttgatg ttggcaacca
     3841 cttcggtgtg cagcggatga acagcaaaca gcgctgtcac cagaaacggc agaccaaagt
     3901 accattttga cgatgtgtaa tttttgaaaa tttttcgcag tacatgaaac agcaaaacgc
     3961 acagcaggcc atatagaaat acattaataa gatgaccaat aaaaggattc aacccgaaca
     4021 attcatactc cactgcgaaa aaaacttgcg aaagcggcct gtagcgccca ccctgcaata
     4081 aattctgttc gccgaaaaca ccagccagcg catccgtagt aagaattttt ccgatgcccg
     4141 aaaatccctg tttggtgaat ttgttatcgc gtatcatcag gtcatcgtcg agcgcatact
     4201 ggtgtccgag cgtattggca tacaaagcaa aagcaaacag aaaaatcaca agcgcctgta
     4261 agcgataatt ctgtgaaaaa gattgttgac ggaatggctt gctatcagat gaaatatgct
     4321 tttgaagcgg ttttttcaaa gctgcagaat tttttttcat atcagcagga attttatgtg
     4381 ctgagagagc atcatttatt gaaaataaat gaacgacaca aagataaaat aaatgcaata
     4441 agtcattaaa aaacgaccgg ctaaaaaaaa gacaggaaaa atgtcgccgc aaacaacaac
     4501 gttaaaacgc ccacaccaag ccaaaattca gattcagcat attggtgttg ccgactcgtc
     4561 tttttcccag gtcgcgaata taggtattgg attcccgaat agtaatattc ggataaacat
     4621 catctttata ccaaacattt ctgattggga aaagatagcc ggataaacta tagtagaaca
     4681 gtaaatgatc ggataaaggc accctgtttc cgaattcata ataaaatccg ctcatagctg
     4741 ttgactggtt taatgcaagc gactgcaccg aatcggggaa atcatttgaa acaggtacgg
     4801 agtagttgga ttccacgtct gtataaagat attctgaatt tttaagagag gccagacaaa
     4861 caccaaagcg gaaataatta tcgattacag aataagcatt ttttgaatga ccttcaagat
     4921 ataaactgaa gatttgtgtt ttataattgg cgattcctga ataataaaaa taatcgttat
     4981 aacgggcgcg gtagaaagtc ccggtaacat tggtaaatgt cgccctggca cctaaagaga
     5041 caccgtttcc ggcaacgaaa ttcacgtctc cggaaatggg agtgcgaata gtaaaaacat
     5101 tatcgtgcga attattcaaa ggattttttt tcaaatcagg agtcagtgag aaattggcac
     5161 tgacaaaaaa acggcttccg agaaaaccat gcacctgagc aaagacgcct gttcccaaaa
     5221 ctatcagtaa agaaaaaatc aatgtctttt tcatttttgt ctcgttttga ttcgttgcat
     5281 agaagaataa accatttgtt ttaccgtgta tttataatcg cggcttttaa cgagcgaaga
     5341 ctcgctgtac acgcgtcttc cggttgtaat atcgtacaaa tagaaataat acaacgtgaa
     5401 atactgaggc gaaagataat aagcaatcat aagtggataa gcaggcggga aaatgagcaa
     5461 ataataagaa agcaaattaa atgcatgctt actgcgcatc gaaacgatac cactgaaagc
     5521 aatataacgg gtgccttctt ccatggccag ttgattcagt ttgtcctgat tggcactgat
     5581 aaaatcttcg ccttcttcat catcttcagg cagttcattt atccaggact gaattagctg
     5641 aaaactgttg tagcggtctt catcggcttc attcatggtg ctgacatcga gcacagtcag
     5701 atctacgccc atcattttcg aagcatcctg caccatggca agatattgtt tttcgtattt
     5761 cgaaacagcg agaaaacgtt cgccactatt tttactttca gtaatgcgca gataaccggg
     5821 atcgaaaaac aaaaccttcg acgttccaac aaacaaagaa tcattatact cgtaactttt
     5881 ctttcgtttt ttatagtccc cctcttcctc caccaatgtt attccctcag catcattata
     5941 cagccgtctg aattccttat catcggcaaa agacagaaaa gcataggtaa aattatcctg
     6001 ctgcagtgct tttttctgat tttgcttgat cttatcatac ttcgatgcag aagaatccag
     6061 cgtcgtcatt tgcgcgcttg aatcgaccgg aacaggagct ttaaatctga aataattgtc
     6121 ttcaatcttg cagttgttca acaaatcaat cattatttca gaagccatat ccaaaatgcc
     6181 agtatcagca ggattcaaca gatgtgcttt ccagatttcg cgtgcgcaga ctgtcaccag
     6241 ttctttattc gacaaattct tgaacatatg aaacatgcgc tgactttcac ctttgatttt
     6301 ggttgaagcc cgtatggccg tcgatttact tttctcattt ttcaaacgca tcagaccata
     6361 ataagctttg gcttttgcac tgttgagata tacgctttca ggatgattct gctgcagaac
     6421 atgaatcagg tataaggcat ccacgtattt gcgatcggtc agatataact gaaccatttc
     6481 gtagcgcgca atttcccgca tgttttgaaa atccttctgt ggtaaaataa aataattccc
     6541 attgtctgag aattcagaaa gcagattttt caaccgctcc cgacgttttt taatattcgg
     6601 atgagttaat ttctcatcat caaaatcttc aatggcagaa ataagggcta cggtatcaag
     6661 ccagcattta tctggaatat ggaaccaatc gtcttcgaac atggcacgcg acaccgggat
     6721 ttcatcaaca ggaaaatcgc tgtacagcaa tacatcgcaa accgattcgg cggcagataa
     6781 tttgtaacca ctggctgaaa aataatcacg gaaccccaat tcatcggcca taaattccaa
     6841 atctttctga tactgaagca tggcaaaaag ccggtccgat tttctgacat tgcggtattc
     6901 gcctacactg cgccggatgc ggttttgctc aacatacgaa tcaataccat gtttctttac
     6961 ataatgaaca atttcgtggg caagcacaaa ggcaagctgg gcctcgtttt caagctgagc
     7021 cagcaggccg atattcacaa tgacaatacc gttaggcaaa caggcagcat tgacttcggg
     7081 atatcttgaa acatagaatc tgaatttttc gcgcaactca ggattgctat tcaataaaat
     7141 atcagcgact ttgttcacat agtttgtgag cggatcacca aacatcagat cgccactacg
     7201 aagcagatag cgtaaataaa aatccgaata tctgataaat tcttttttac ttttaatatc
     7261 cttatagctt gcatttggat cgatgctgcc gacatcggcc gttgtcacca agaattcatc
     7321 gctgtaaaac tccgcaggca attcgccact gcagcgaatg ggctgatatt ggttgaagtc
     7381 ctgggccgtc agaagcaatg ctgtcaacca cgaaacaaaa aataaaacag tccgtaacat
     7441 ttattactca ttttggacaa atataaatga aatatataca cttgcatctt taactaacag
     7501 aagtaaacaa gtgtttcggc ttcaacatat tactactgga cgattaaaca tccacttcag
     7561 taacagatat atcagccccg cgttttttta aactgtctga aattcatttc agtgacaaaa
     7621 cgatattcca cgtccgccgc tgcggaataa taccaaatgg aaataaacat ttcggaagac
     7681 tccggaggag tcacatctct gtagaaaaca attcaaaaat ctacgacccg ccagctggcg
     7741 ggtcgaataa aaaaagcttc gacctctgaa atacatgtct gcatgcgatc caccagtcat
     7801 gtgctgaacc gaagcctgcc ctcctgactt cgccactggg acaaccattt ttttctgaag
     7861 tttttgagga aattcatcca ataaaaacaa aggaagagtg taaatactta ttatgaattt
     7921 ttcctgaccc gccagctggc gggtcaaaca ataatagccc ggcctgctgt gtcacgtttt
     7981 acgtgaaatg gccgggtaaa agatagaaca aaaaacgacc tcggagaggt cgaataaaac
     8041 gaaaatattg aaacaatgtg ttttaaaaac
//
LOCUS       DAOPKE010000116         8047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9501_length_8047_cov_12.060435, whole genome shotgun sequence.
ACCESSION   DAOPKE010000116 DAOPKE010000000
VERSION     DAOPKE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9501_length_8047_cov_12.060435"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKY63_11370"
     CDS             complement(<1..430)
                     /locus_tag="PKY63_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPF01259.1"
                     /translation="MILLIDPDKFDEEKTSLFFSHHSGLMDMIFVGGSLLSANRTSFV
                     IAALKKLTQIPVVLFPGNSMQLSANADAILFLSLISGRNAEYLIGQHVQAAPLIYNSE
                     LEVIPTSYMLIDGGNRTSVQYISQSMPIPADKTDIAVATAL"
     gene            535..1059
                     /locus_tag="PKY63_11375"
     CDS             535..1059
                     /locus_tag="PKY63_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01260.1"
                     /translation="MYKVLMIIAVLLVSANVNAQMNQITFDTTAQKDILIGECNREGM
                     LHPLFAEYYNTEYGAYSPVPETLMLLSAFGHDYKITIVMGSWCGDSHEQVPRFLKTAD
                     LAGFPEENIRIICVNKKKTAPGFEEELIPLNIEKVPTFIFNSMAADGREIGRIIETPL
                     ESLETDWLKILENL"
     gene            1097..2194
                     /gene="meaB"
                     /locus_tag="PKY63_11380"
     CDS             1097..2194
                     /gene="meaB"
                     /locus_tag="PKY63_11380"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPF01261.1"
                     /translation="MNKDKKPFESALKVQQGIEQPSQTNEEAIRKMAGKKSKIPSFDE
                     LHEGILSGNRVLLSRAITLIESSKHDHQALAQALIEKCLPHSGNSIRIGITGVPGVGK
                     STFIESFGNHLTAIGKKVAVLAIDPSSQISKGSILGDKTRMEKLSSNPDAFIRPSPSA
                     GTLGGVAQKTRESIVLCEAAGFDIIIVETVGVGQSETAARSMVDFFLLLQLPGAGDEL
                     QGIKRGIMEMADAIFVNKAEGDYLNKAKIAAAEIKNALHLMPAHQSGWITQVGLCAAL
                     SGKGIGEVWDTILDYISLAKNTGYFDKFRHEQEVNRYHQAIMEMLQSDFLGSSDVRHL
                     NTDLEKKVFASALSPYNAAKQIVSKFYETLK"
     gene            2312..2959
                     /locus_tag="PKY63_11385"
     CDS             2312..2959
                     /locus_tag="PKY63_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01262.1"
                     /translation="MLRLFKKNIRFEFDVNIDTRPFGSDLSLAQISIIKKYHRKQKRT
                     IEKYASEVANKCKHDFRDNIKKIKTSCLELCVLYHHFFTNEFEKIKADAAVSRLYFST
                     IYHNFSDLEERLHELDYANMADFIRVKKNYYKEVHDRGSRLELSHYYYHTFAKFPLMQ
                     PENYKTLPPFFDYIDSWLNYIFFLDFTARIRMEAEEMSKAMAVLRPAHVFSHAHA"
     gene            complement(3027..3785)
                     /gene="rsmA"
                     /locus_tag="PKY63_11390"
     CDS             complement(3027..3785)
                     /gene="rsmA"
                     /locus_tag="PKY63_11390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPF01263.1"
                     /translation="MLPVKPKKSLGQHFLNDQNIARKIAGSLQFANENALVVEIGPGS
                     GFMTQFLVDMPYRLLLIETDRESVEYLNQRFPQLSDSIIHRDVLKVHFSEYSEKLISV
                     IGNMPYNISGPLLFMALDQREMLNEWIGMLQKEVADRIVAKPSTKAYGILSVLLQAYF
                     DAEMLFSVPPSVFIPPPKVNSAVIRLVPKKTDIPQCDYSQLKMLVKTAFNQRRKTMRN
                     SLKNFFSADILTNPVFNKRPEELTWREFEELLQL"
     gene            complement(3789..5597)
                     /locus_tag="PKY63_11395"
     CDS             complement(3789..5597)
                     /locus_tag="PKY63_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01264.1"
                     /translation="MIFLFISLFVSFSAFTQETEKELLAREYMSSGEYEKAVVLYEEI
                     YSKNTSTYIYDSYLECLLKTENWKDAEKLTQKQKRNNPSVPRFHVDEGYVQLLQENTK
                     HAEKIFNEAIQWAVSTERLTSDLAAAFQVRELDNWAIQTLLAGKKIFPSSGPLRIQLA
                     ETYLRAEKYPQMVDEYFSLLDNPMYGITEIQKQMQSLLMEDRDGNISEIFRNKLLERV
                     QKDPSSLDFNRLLIWYYEQNDDFDRAFIQAKAFDRRTKNDGDLTYDVAQLCVSNKKFS
                     VAEEALAFIVSLGSEKLLFSDANDLLLEVKYLKLTANPLSTAEEYSLLADELKTALNT
                     SVSRRNRYSMLMRLAYIEAYYISQPDAALSHLNEIINASGYQPVEYADAKLLQGDVML
                     LKGEVWEASLLYSQVEKAYKHDTIGFYAKFRNARLYYFLAEFDYASAQLEILRGATSK
                     LIANDAMELFLVIQENVDYDSSYVPLEKFAKADMLTFCLQYDDALLVLDTILKSFPAH
                     PVLDDAVYKRAEIYMALKQYDKAASELKVILSAYYFDILADNALFMLGNIYESYIPDK
                     EQAMKYYLQLVTDFPGSILVNDARQRYRALRGDNLN"
     gene            complement(5603..6151)
                     /locus_tag="PKY63_11400"
     CDS             complement(5603..6151)
                     /locus_tag="PKY63_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01265.1"
                     /translation="MKNVIVVSLVVLLFFVSCRPAYIRRGVKEISVERERLNEAKQKF
                     EVLDTAQVHGIYLSYIGKIDSINKYFKDQYSEGAWKLMTEFGQLKKPLKTYFEQYVSV
                     EKDFFFSLEQLADLEYDLKSKNISKELFLVYIQQEKDAIGQLIMKSNLISDNAVRNVN
                     HYNEISPKIDSLVIVFKAGLNK"
     gene            complement(6161..7432)
                     /gene="serS"
                     /locus_tag="PKY63_11405"
     CDS             complement(6161..7432)
                     /gene="serS"
                     /locus_tag="PKY63_11405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPF01266.1"
                     /translation="MLTIQLIRQETEYVKERLAVKNFKNIALVNEIIVLDDNRRKLQL
                     ERDDAQNEMNSRSREIGIAIKEKDMAKAESLKNETAELKEKIRILNQQHDDVVSELNN
                     KLVLLPNLPHASVKPGHSAEDNEIIHSEGTIPELGSDAVPHWDLTSKYKLIDFELGVK
                     ITGAGFPVYTGLGARFQRALIYYFLTEAIKAGYTEIQPPLMVNEASGYGTGQLPDKDG
                     QMYYANEDNLYLIPTAEVPVTNIYRDVILKAEDLPVKNVAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQISTPEKSYEVLEEMRAHVASLVRGLGLPFRVLRLCGGDISFTSA
                     LTYDFEVFSAAQQKWLEVSSVSNFESFQSNRMKLRYKDTEGKTQLAHTLNGSALALPR
                     IVASLLENNQTPDGIRVPEVLVSFMGTDIIK"
     gene            7660..>8047
                     /locus_tag="PKY63_11410"
     CDS             7660..>8047
                     /locus_tag="PKY63_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF01267.1"
                     /translation="MIRRTFSSIGEYILLMLRVFKRPDRLEQFRNQVFIEFKLLGIES
                     LGIIAIISIFMGAVLAMQTAYSIDSPLIPRYTIGYITRSSIVLEFSATIMSLIMAGKV
                     GSRIASELGTMRVTEQIDALDVMGINS"
BASE COUNT         2351 a         1831 c         1621 g         2244 t
ORIGIN      
        1 ccagcgcagt cgccacggca atatcggttt tatcggccgg aatcggcatg gattgagaga
       61 tgtattgtac cgatgtgcgg ttgccgccat caatcagcat atacgatgtt ggaatgactt
      121 ccagttcact gttataaatc agcggtgccg cctgcacatg ctgaccaatg agatattcag
      181 catttcggcc cgagatgagc gacagaaata aaatggcatc ggcattagcc gaaagctgca
      241 tcgaatttcc gggaaacaaa accaccggaa tctgtgtaag tttcttcagt gcagcaatca
      301 caaacgacgt tctgttggcc gaaagcaaac taccgccaac aaaaatcata tccatcaacc
      361 cactgtgatg actgaaaaac aggctggttt tttcttcgtc gaatttgtcc ggatcgatta
      421 aaagtatcac ccgtttctcc ggtctcagaa ataagtcgta tatttgatta tgcttttgca
      481 tgacgtgtat tgaatagcca aagataacaa acctttttct aacaccagca accgatgtac
      541 aaagtcctga tgattatcgc tgtactgctc gtttcggcca atgtaaatgc gcaaatgaac
      601 cagataacct ttgataccac cgctcaaaaa gacattctga ttggtgaatg caaccgcgaa
      661 ggaatgctgc acccgctttt tgcggaatat tacaacaccg aatacggcgc ctattcccct
      721 gttccggaaa cactcatgct gttatcggct tttggacacg attacaaaat taccatcgtc
      781 atgggaagct ggtgcggcga cagtcacgaa caggtgccac gcttcctgaa aacagctgat
      841 ttggccggat ttcctgaaga aaacattcgg ataatctgtg tcaacaagaa aaaaacggcc
      901 ccgggttttg aagaagaact gattccgctg aatattgaaa aagtgccgac ttttattttt
      961 aattccatgg ctgcggatgg gcgcgaaatc ggtcgcatca tcgaaacacc gcttgaatcg
     1021 ctcgaaacag actggttgaa aatattggaa aacttataat gtttttttcg cggcacgctg
     1081 ctaattttgt ccggttatga ataaagataa aaagccattt gaatcggcac tgaaagttca
     1141 gcaaggcatt gagcagccgt cacaaaccaa cgaagaagcg attcgcaaaa tggccggcaa
     1201 aaaatcaaaa attccatcgt tcgatgaatt acacgaagga attctatccg gcaacagggt
     1261 tttgctcagt cgtgcaataa cccttattga aagctcaaaa cacgatcatc aggcactggc
     1321 tcaggcactg attgagaaat gtctgcccca ctcgggaaac agcatccgca ttggtattac
     1381 cggtgtgccg ggtgttggaa aaagtacttt cattgaatcc tttggaaatc acctgaccgc
     1441 catcgggaaa aaggtggctg tactggccat tgatccgtct agtcagattt cgaaaggcag
     1501 cattctgggc gataaaacaa ggatggaaaa actttcatcc aacccggatg catttatcag
     1561 gccctcgcca tcagcaggaa cgctgggcgg cgtggcacaa aaaacacgcg aatcgattgt
     1621 tctgtgcgag gcagccggat tcgatattat cattgttgaa acggtcggcg ttggtcaaag
     1681 cgagacagct gcccgttcca tggtcgattt ctttttattg ctgcagttgc cgggcgccgg
     1741 cgacgaacta cagggcatca aacgcggcat tatggaaatg gccgatgcca tctttgttaa
     1801 taaagctgag ggcgattatc tgaataaagc aaaaattgcc gcagctgaaa tcaaaaacgc
     1861 acttcatctg atgcctgccc atcagtcggg atggatcacg caggtcggac tctgcgcagc
     1921 actatctgga aaaggcattg gcgaagtatg ggacaccatt ctcgattata tttcgctcgc
     1981 aaaaaacacc gggtactttg ataaattccg ccacgagcag gaagtcaacc ggtatcacca
     2041 ggccattatg gaaatgcttc agtcagactt tcttggctcg tccgatgtcc gacatctgaa
     2101 tacagacctc gaaaagaaag ttttcgcatc tgcattatca ccttacaatg cagccaagca
     2161 aattgtttcg aaattctacg aaacactgaa gtaaaaccac acttgttaaa ctttctacga
     2221 aaacgcagtt ttaatcactt ttattttgcg tttcatcgat atttttatta tatttataaa
     2281 ttgatagaaa taattaaaaa atacagttgc catgctccgt ttattcaaaa aaaacatccg
     2341 gtttgagttt gatgtaaaca ttgacaccag accatttggg tcagatcttt cactggctca
     2401 gataagtatt ataaaaaaat atcaccgcaa acaaaaacgc actattgaaa aatatgcttc
     2461 tgaagtcgca aataaatgca aacatgactt cagagacaat ataaaaaaga tcaaaacatc
     2521 gtgtcttgag ctctgtgtac tctatcatca tttttttaca aatgaatttg aaaagattaa
     2581 agcagacgca gccgtatcgc gcttgtattt ttcaacaatc tatcacaact tcagcgatct
     2641 ggaagaaaga ctccatgaat tggattatgc aaacatggcc gatttcatca gggttaaaaa
     2701 aaactattac aaagaagtac atgaccgcgg atcacgtctc gaactatcac attattacta
     2761 tcacacattt gcgaaatttc cactgatgca gccagaaaat tacaaaacgc ttcctccgtt
     2821 tttcgactac attgattcat ggctcaatta cattttcttc cttgatttta ccgcccgtat
     2881 tagaatggaa gcagaagaaa tgtcgaaagc aatggctgta ctgcggcccg cacatgtatt
     2941 ctctcatgct catgcatgaa gctatcagag aacaaacagg tgtttgatgt tttttgactg
     3001 tcgcggaaat tttccgcgac agttttttat aactgcagca actcctcaaa ctctctccag
     3061 gtgagctctt cggggcgttt attaaaaacc ggatttgtaa gaatgtcggc agaaaaaaag
     3121 tttttcaagg aatttcgcat cgtcttgcgc ctctgattga aagccgtttt tacaagcatt
     3181 ttcaattgtg aataatcgca ttgcggaata tctgttttct ttggcaccag tctgatgaca
     3241 gcagaattta ctttgggtgg cggtataaaa accgatggag gaacactaaa caacatttct
     3301 gcatcgaaat aggcctgcaa caacacactc aaaataccat acgctttggt agatggcttt
     3361 gcaacaatgc ggtctgccac ttctttctga agcattccaa tccactcatt cagcatctca
     3421 cgctgatcga gtgccatgaa cagcaatggc ccggaaatat tatagggcat atttccaatg
     3481 acactgatta atttttcgga gtattctgaa aaatgcactt tcaaaacatc acggtggata
     3541 atactgtccg aaagctgtgg aaaccgctga ttcaaatact caacactctc acgatctgtt
     3601 tcaattaaaa gcaatctgta tggcatatca accagaaact gtgtcataaa accgctgccc
     3661 gggccgattt cgacaacaag cgcattctca tttgcaaatt gcagactgcc ggcaattttg
     3721 cgtgcaatgt tctgatcgtt cagaaaatgc tgtcctaatg atttttttgg tttgactggc
     3781 agcatgaatc aattcagatt gtcgccgcga agcgctctgt agcgctgtcg ggcgtcgttt
     3841 accaaaatgc ttcccggaaa atcggtaacc aactgcaagt aatatttcat agcttgttcc
     3901 ttgtcgggaa tataactttc atatatattg ccaagcatga aaagcgcatt atcagcgaga
     3961 atatcgaaat aataggcgct cagaataact ttcagttccg acgctgcttt atcgtattgc
     4021 ttcaaagcca tataaatttc agcacgctta taaacagcat catcaagtac cggatgtgcc
     4081 ggaaaacttt tcagaatggt gtccagaacc agcaaagcat catcatactg aagacaaaat
     4141 gtaagcatat ctgctttggc aaatttctca agcggaacat aagaactgtc ataatccaca
     4201 ttttcctgaa tgaccagaaa caactccatg gcatcgttgg caatcagttt tgaagtggca
     4261 ccacgcaata tttcaagctg tgccgaggcg taatcaaact cagccagaaa ataatacaaa
     4321 cgcgcattgc ggaactttgc ataaaaaccg atggtatcat gtttatacgc tttttcaacc
     4381 tgagaataca acagagaggc ctcccagact tcgcctttca gcaacatcac atctccctgc
     4441 agcaatttgg catcggcgta ttccacgggc tgataaccag aagcattgat tatttcattc
     4501 aggtgcgaca aagcagcatc gggttgcgaa atataatagg cctcaatata tgcaagtcgc
     4561 atcagcatgc tgtaacgatt gcggcgactt accgaagtat tcagggctgt cttcaactca
     4621 tcagccagca gactgtattc ttctgctgtt gacaaaggat tggccgttaa tttgagatat
     4681 ttcacttcaa gcaaaagatc attggcatca gaaaacaaaa gtttttcaga acctaatgaa
     4741 acgatgaatg caagggcttc ttcggcaaca ctgaattttt tattcgaaac acaaagctga
     4801 gcaacatcgt aggtgagatc gccatcattt tttgtacgtc tgtcaaaggc ctttgcctga
     4861 ataaatgcgc gatcaaaatc atcattctgt tcgtaatacc agatgagcaa acgattaaaa
     4921 tccaaactgg aaggatcttt ctgaacgcgc tccagaagct tgttgcggaa tatttcagaa
     4981 atatttccgt cacggtcttc cattaacaaa gactgcattt gtttctgaat ttccgtaatt
     5041 ccatacattg gattatcaag caaagaaaaa tattcatcca ccatttgcgg atacttttct
     5101 gcacgcagat atgtttcggc caattgtatt cgcaaagggc ccgaagatgg gaatattttt
     5161 ttaccggcaa gcaatgtctg tatagcccaa ttgtcaagtt cacgcacctg aaaagccgca
     5221 gccagatcac ttgtaagtcg ttctgttgaa acagcccact gaatggcttc gttaaatatc
     5281 ttttcagcat gctttgtatt ctcctgaagc aactgcacat aaccttcatc cacatgaaag
     5341 cgtggaaccg acggattgtt gcgcttctgc ttctgggtca gcttttcagc atctttccag
     5401 ttttcagttt tcaacaaaca ttcaagataa ctgtcgtaaa tataagttga tgtatttttg
     5461 gaatatattt cctcatacaa aacaacggcc ttttcatatt cgcctgaact catgtattcg
     5521 cgtgcaagca attctttttc ggtttcctgg gtaaaagcag aaaacgacac aaacagcgat
     5581 atgaaaagaa atatcatcct cattacttat tcagtccggc cttgaaaaca atcaccagac
     5641 tatcgatttt cggactgatt tcgttataat gattaacatt acgaacagca ttatctgata
     5701 tcagattact tttcattatc aactgtccga tggcgtcctt ttcctgttga atatatacaa
     5761 gaaacaactc tttactgatg ttctttgatt tcagatcgta ttcaaggtcg gccaactgct
     5821 ccaatgaaaa gaaaaaatcc ttttcaaccg aaacatattg ctcgaaatac gtcttgagcg
     5881 gttttttcag ctggccaaac tcagtcatga gcttccatgc gccttcggaa tattgatcct
     5941 tgaaatattt gtttattgaa tcaattttcc cgatgtatga caaataaatt ccgtgaacct
     6001 gagctgtatc aagtacttcg aatttctgct ttgcttcgtt caatcgctca cgctccacac
     6061 tgatttcttt cacacctctc cggatatagg caggacgaca ggaaacaaaa aacagaagaa
     6121 caaccaacga aacaacaata acatttttca tgacaacatt ttatttaatg atatccgttc
     6181 ccataaagga aacaaggact tccggaaccc tgattccatc aggagtttga ttattttcga
     6241 gtaatgaggc aacaatgcgg ggcaatgcca gcgcgcttcc gttcaatgta tgagccaatt
     6301 gcgttttacc ttccgtatct ttgtaacgca acttcatgcg gtttgactga aatgattcaa
     6361 aattggaaac cgaacttact tcaagccatt tctgttgtgc cgcgctgaat acttcaaagt
     6421 cataggtcaa tgccgatgta aaactgatgt cgcctccgca caggcgcaaa accctgaacg
     6481 ggagcccaag tccacgcacc agcgatgcaa cgtgtgcacg catctcttca agcacttcat
     6541 acgacttttc gggtgtcgat atctgaacga tttcaacttt atcgaactga tgcaaacggt
     6601 tcagtccgcg cacgtctttg ccgtatgagc cggcctcacg acgaaaacag gcactatagg
     6661 ccacattctt caccggcaga tcttctgctt tcagaatcac atcgcggtaa atattggtta
     6721 ccggcacttc ggcggtagga ataaggtaca gattatcttc attggcataa tacatctggc
     6781 cgtccttatc gggaagctgt ccggtgccat atcccgaagc ttcattaacc atcaatggtg
     6841 gctgaatttc ggtatatcca gctttgatgg cctccgtgag aaaatagtaa atcaacgccc
     6901 gctgaaaacg cgcacccaat ccggtgtaca caggaaaacc cgctccggtg attttaacgc
     6961 ccagttcaaa atcaatcagc ttgtatttcg atgtcagatc ccagtgcgga acagcatctg
     7021 atccaagttc aggaatagtg ccttcgctgt gaataatttc attgtcttcg gccgaatgtc
     7081 cgggctttac cgaagcatgc ggcagattgg gcagcaacac cagtttattg ttcaattcgc
     7141 ttacaacatc atcatgctgt tgattcaaaa ttctgatctt ttccttcagc tcagcggttt
     7201 catttttcaa tgattcggct ttggccatgt ctttttcttt gatggcaata ccgatttccc
     7261 tcgaacggct gttcatctca ttctgtgcgt cgtcgcgctc cagctgcagc tttcggcggt
     7321 tatcatcaag cacaatgatt tcgttaacca aagcaatatt tttgaaattc ttcaccgcaa
     7381 ggcgttcctt cacgtattcc gtttcctgtc ttatcagttg tattgttagc atgaaagtat
     7441 tttttgcaaa aataccaatt taattcgtct cttgaatagt ggagttataa gttattgaat
     7501 ttgggatgta attagggctg aggttaagcg tcacgcgtcc ggagacgcac ggcgacgttg
     7561 ttggatttag gggctgaagg cacattaaga ctgccctgca tatattttca agaccattct
     7621 caaaatctga tagttaattt tttttcctaa attcgccaca tgattcggag aacattttca
     7681 agcattggcg aatatatact tctgatgctt cgggttttta aacggcccga tcgtctggag
     7741 caattccgca atcaggtttt catcgaattc aaactattgg gcatcgaatc gctgggcatc
     7801 attgccatta tcagcatatt catgggcgcc gtattagcca tgcaaacggc gtacagcatc
     7861 gacagcccgc tgattccgcg ctataccatt ggctacatca cgcgttcctc catagttctc
     7921 gaattttcgg ccaccatcat gagtctcatc atggctggaa aagttggctc aagaattgcc
     7981 agtgaattag gaaccatgcg ggttacagag caaatcgacg cgcttgatgt catgggaatc
     8041 aattccg
//
LOCUS       DAOPKE010000117         8007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9569_length_8007_cov_13.651157, whole genome shotgun sequence.
ACCESSION   DAOPKE010000117 DAOPKE010000000
VERSION     DAOPKE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9569_length_8007_cov_13.651157"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="PKY63_11415"
     CDS             complement(<1..1827)
                     /locus_tag="PKY63_11415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF01268.1"
                     /translation="MEFNETNLSQPLLLAVEELGFKTLTPIQEKTIKLILENDHDMIG
                     LAETGTGKTAAFGLPLLEKTDVAEKAVQTIILSPTRELCMQIAKDLENYAKFMNGVHI
                     TAVYGGASIDTQITKLKRGSHIIVGTPGRTVDLIKRRALKLNNVRYLVLDEADEMLNM
                     GFKEDLDFILEQTPAEKQTLLFSATMPREIRNIAETYMNSPEEVSVGKRNMGAENVSH
                     QYYVTHAKDRYVALKRIVDINPKIYGIIFCRTRTETKEVADKLIQDGYNADALHGDLS
                     QAQRDFVMNRFRIRHLQLLIATDVAARGLDVDNLTHVINYNLPDELEAYIHRSGRTGR
                     AGRKGVSISLLHTRETRKVRDLERMVGKQFERKMVPSGFDVCEKQLFNFIDRVENIEV
                     DEKQIEQYLAPIYKKLDWLQREDLIKHFVSVEFNRFLEYYKNAPDLNANVDMSRDGKF
                     GRDSRDGRDRRSSRDDRDGRDRRDSRNDRDSWDDKPKRDRSMAYSRFHINVGEKEGLS
                     VPRMIGLINDYCRRRDIPIGKIDVLRKFSFFEVDAKFESHVLSSFAHADFNGTPLEVT
                     LSKPDTHGSKEKSKPAFGGKKKSFGDDKSWKKDKKPSAGKRRR"
     gene            2057..2767
                     /locus_tag="PKY63_11420"
     CDS             2057..2767
                     /locus_tag="PKY63_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF01269.1"
                     /translation="MENLITHSGYNITQLLSGHSNSWLVQKNDLSILVDSGKTKSHSL
                     LMQSLNTALHHQNGLHYLMLTHTHYDHCQSAAAIKNLYHCNIIVSENAVPSITAGYTH
                     LPRGTNYVTHVLAKAGHALMKKKFMYAPFNADITISNDYVFPAPDADIRIITTPGHSA
                     DSISLIVDNEVAIVGDAMFGIFRNQVFPPFADDIPEMIRSWGKLLDTGCTTFLPGHGR
                     AISRELLEKEYGIFSKTV"
     gene            complement(2938..3486)
                     /locus_tag="PKY63_11425"
     CDS             complement(2938..3486)
                     /locus_tag="PKY63_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPF01270.1"
                     /translation="MTSNETISGTSILIDNDFVNCTFKNCSFAGQDLGKFNFESCEFI
                     GCDLTMCKMENTAYSEVVFNDCKLQGVEFGKCSKYVFSVTFEKCVLNYAVFLKNNLKK
                     MWFMHCSIREAFFAECDLTQALFDNCDFDMTSFERCNLSGCDFRTSSNYIIFPSQNKL
                     KKAKFAYPGVLGLLADLDIVVE"
     gene            3855..4922
                     /gene="rsgA"
                     /locus_tag="PKY63_11430"
     CDS             3855..4922
                     /gene="rsgA"
                     /locus_tag="PKY63_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPF01271.1"
                     /translation="MKPQDYGYSEKHEQFRKENNLTEFSVARVIAEHKERYIIVSDQG
                     ECEAEITGNMRFSAGGRENFPAVGDWVAATIYDSGMALIHKIFPRSSVIQRQAIGAAG
                     DIQIIAANVDFAFLVQGADRDFNINRIERYLTICYNSRIEPIVVITKIDLFEENHISE
                     LLNSIRQRLPGILVLAISSETQQGYDELNSFFLKGKTYCMLGSSGAGKSTLLNHLIGA
                     ETMKTRSLSDSTSKGRHTTTHREMFVLPNGALMIDNPGMKEVGIANASAGLEATFDEI
                     VQLAKQCRFKDCTHTSENGCAVRAAVDSGDIDPASLENFLKLEKEKEHYESTVAERKQ
                     KDRAFGKMLKNYKKGKYKDNF"
     gene            4952..5245
                     /locus_tag="PKY63_11435"
     CDS             4952..5245
                     /locus_tag="PKY63_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPF01272.1"
                     /translation="MTSTISICGLDCNTCPEYGKQCQGCVASQGKIFWAVHYNMDKCP
                     LHKCCTDEHNYAHCGQCDQLPCKTYFDMRDPSYTEEQHLEGIKTRVKTLRKLN"
     gene            complement(5247..5858)
                     /locus_tag="PKY63_11440"
     CDS             complement(5247..5858)
                     /locus_tag="PKY63_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPF01273.1"
                     /translation="MKLFSLIFALVITGAVLVSCYDHGDHPADFSYEEVNLIASGSAT
                     EKMRVLNYFIYEDSLILRNNSGNVNEFGSFYLKMLKDRMYKTVTNPANPGVGLAAPQV
                     GVNKRVIWVKRYDKTGAPFECYLNSYITAYSDTVKLRSDGCLSIPGVSGSSWRAIWVD
                     VVYDTPDGIRCSERISHEYTAHIFQHEIDHLNGFVFLDRAELP"
     gene            complement(5860..6492)
                     /locus_tag="PKY63_11445"
     CDS             complement(5860..6492)
                     /locus_tag="PKY63_11445"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPF01274.1"
                     /translation="MFSRDFIAKLFQNHPLPGSIAHAEMMPEGRSMTMFSESEPFSHS
                     AVLLLLFNHLNSTRIVFIKRAEYDGVHSGQIAFPGGRSEPEDVNFWQTAVRETLEETG
                     IHFPVSYIGQLSDLYVPLSRFLIHPFVGFVDKKPKYMPDLHEVSAVFDADVLWLANPE
                     SRGVFHYKSGHLTGTAPCFRADNHVIWGATAMILNEFLLLLQKNACCKLK"
     gene            6586..7044
                     /locus_tag="PKY63_11450"
     CDS             6586..7044
                     /locus_tag="PKY63_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HPF01275.1"
                     /translation="MKKIFSVLAAAVIAFAFTSCGGGADTPEKVAEKFLQHIAKSEFK
                     DAKELATGDAVKTIETLESFASMSEMGGEKPEAKEVKIENMKCDTKEDAAACTYKQDG
                     KDGNIDLKKVDGAWKVSSFPKETTGGDETEEVDMESDTTATDDVTEEVAE"
     gene            7073..7720
                     /locus_tag="PKY63_11455"
     CDS             7073..7720
                     /locus_tag="PKY63_11455"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPF01276.1"
                     /translation="MKKGLLTILIFIAAFVLFLSTYDLRSEQKQQYESYDLTPDEYAM
                     LKDGDLILRHGYGLVSDAIANSMNEENHVSHVAVLVKSDTAKNGFRIVHCVSQSLSPW
                     DGVQEQTFPRFIHDSQRNSIMVVRFKGANDSTRHKISEKALDYLNQRVTFDHAFDIAD
                     SSTFYCSELPYMIFKNEFGVDIFKDKMNEQQDHMKFSNFWNPDNFEVIINHNTKR"
BASE COUNT         2382 a         1810 c         1659 g         2156 t
ORIGIN      
        1 gcggcgtctt ttaccagcac ttggtttttt atcttttttc cacgacttgt cgtcgccaaa
       61 ggattttttc tttcctccga aagcaggctt ggatttttcc tttgagccat gtgtgtcggg
      121 tttcgaaagt gtcacttcaa gcggtgttcc attaaaatct gcatgagcaa acgagcttaa
      181 cacatggctt tcaaacttgg catctacctc gaaaaaagaa aatttgcgca ggacgtcaat
      241 tttgccaatc gggatatcac ggcgacggca ataatcgttg ataaggccaa tcatgcgcgg
      301 aacggatagt ccttcttttt ctcccacatt gatatggaaa cggctgtatg ccatcgagcg
      361 gtcgcgtttt ggtttgtcgt cccacgaatc tctgtcatta cgactatctc tgcgatcgcg
      421 gccatctctg tcgtcgcggc tgcttctgcg atcacgtccg tcgcggctat cgcgtccgaa
      481 ttttccgtct cttgacatgt caacattggc attcagatcc ggtgcgtttt tgtaatattc
      541 aaggaaacgg ttgaattcaa cagatacaaa atgtttgatc agatcttcgc gctgaagcca
      601 gtcaagcttt ttgtaaatcg gcgccagata ttgctcaatc tgtttttcat cgacttcaat
      661 attttcgaca cgatcaataa aattgaacag ctgtttttcg cagacatcaa atccactcgg
      721 aaccattttg cgttcaaact gcttcccgac catacgctca agatctctca ccttgcgggt
      781 ttcgcgcgta tgaagcaacg aaatcgaaac ccccttacgt cccgcccgac cggttcttcc
      841 acttcgatgg atgtaagctt caagctcgtc cggaagattg taattgataa catgtgtcag
      901 attatccaca tccagaccac gggccgcaac atcagtagca atgagcaact gcagatgacg
      961 gatgcggaaa cggttcataa caaaatcgcg ctgtgcctgc gacagatcgc catgtaatgc
     1021 atcggcattg tagccatcct gaatgagttt gtctgcgact tctttcgttt cagtgcgtgt
     1081 gcggcaaaaa atgattccgt aaattttcgg attaatgtca acaatacgct ttagcgcaac
     1141 atatctgtct tttgcgtgtg tcacataata ctgatgcgat acattttcgg cacccatatt
     1201 tcttttcccc accgaaactt cctctggact attcatgtag gtttcagcaa tattgcggat
     1261 ttcgcgcggc atggttgctg agaaaagcaa tgtttgtttt tcagctggtg tttgttcaag
     1321 aataaaatca aggtcttcct tgaagcccat attcagcatt tcatcagctt cgtcaagtac
     1381 gagatagcga acattattca atttcaacgc acgacgttta atcaggtcaa cggttctgcc
     1441 aggtgttccc acaatgatat gagaacctcg tttgagcttt gtaatctggg tatcaatgct
     1501 ggctccacca tacacagcgg taatatgaac accgttcata aacttcgcat agttctcaag
     1561 gtctttcgca atctgcatac aaagctcacg ggtcgggctc agaatgatgg tctggactgc
     1621 tttttcagca acatcggttt tttcaagcaa aggcaatccg aacgcagcag tttttccggt
     1681 tccggtttca gccagcccaa tcatatcgtg gtcgttttca agaataagct taatggtttt
     1741 ttcctgaatg ggagtcagtg ttttaaaacc taattcctct accgctaaca gcaagggctg
     1801 actcaggttt gtttcgttaa attccataca attatgttag ataagtccct ctgtattgcg
     1861 ctcttcggga ccaacttcta tgtaatccgc tgtaaaacaa ttcaatttcg ggggtgcaaa
     1921 ggtacgcaaa atattcatac gctaagagat tgatgtgtga aataacagta ggcagataat
     1981 gctttccgca tgcaatcaat gattgaatga aatatattcc ggttttatcc tttttacttt
     2041 actttgcatt taattcttgg aaaatttgat cacgcacagc ggatacaaca tcacgcaatt
     2101 gttgtctggc catagcaatt catggcttgt tcaaaaaaac gacctgtcca tactggtcga
     2161 ttccggtaaa acaaaatcac attcactgct gatgcaaagt ctgaacaccg cactgcatca
     2221 tcaaaacggc ttgcactatc taatgctgac gcatacgcat tacgatcatt gtcagtcagc
     2281 agcggcaata aaaaacttat atcattgcaa catcattgtc tctgaaaatg ctgtcccatc
     2341 catcactgca ggatatactc acctgccgcg cggcaccaat tatgtcaccc atgttttagc
     2401 aaaagccgga catgcattga tgaaaaagaa attcatgtat gcacctttca atgccgatat
     2461 taccatcagc aacgactatg tattccccgc tccggatgcg gatatcagga taataaccac
     2521 accgggtcac tcagccgatt ccatcagtct gattgtggat aatgaagtgg ccattgtggg
     2581 cgatgcgatg tttggaattt ttcgcaatca ggtgttccca ccctttgccg acgacattcc
     2641 ggagatgatc cgcagctggg gcaaactgct cgataccggc tgcaccacat tccttccggg
     2701 ccacggccgc gccatcagca gagagctatt ggaaaaagaa tacgggattt tttctaaaac
     2761 agtctaatat gtctagcatt gctgggcatg cgtctcctga atcatgaaca aagaacaagg
     2821 aatgatgaat gaagaagtaa gtacaaagtg aagtatcctt catacttttc acttccttgt
     2881 tccttgttca gagttcggtg ttcccttcat acttctcaca tccttgttcc ttgttcatta
     2941 ttcaacaaca atatccagat ccgccagcag tcccagcact ccgggatagg cgaatttcgc
     3001 ttttttcagt ttattctgcg aaggaaaaat aatgtaattc gatgatgtcc ggaagtcgca
     3061 acccgacagg ttgcagcgct cgaagctggt catatcgaaa tcacaattat caaacaaagc
     3121 ctgcgtcaga tcacattctg caaaaaaagc ttcgcgaatg gaacaatgca tgaaccacat
     3181 ttttttcaga ttgtttttca gaaaaaccgc ataattcagc acacactttt cgaaagtcac
     3241 cgaaaaaaca tatttcgaac atttaccgaa ctcaactccc tgcagcttgc aatcattgaa
     3301 aacaacttcg gaatacgccg tgttttccat tttgcacatg gtcagatcgc agccgataaa
     3361 ttcgcaggac tcaaaattga atttgcccag atcctgacct gcgaagctgc aattcttaaa
     3421 agtacagttc acaaaatcat tgtcgatcag aatggatgtg ccggagatag tttcgttgga
     3481 ggtcattcga aattttttgg tgtacaaact tacttgaaat aatctgaata tgaaatattg
     3541 atatgcgagt ctgtgcaaag aaattgtatc aggtttcagt ccgcagcggc ggatcagcaa
     3601 tcagctctga gaaggagctg tgaagtattg tttatatacc taaggatgtc ttaaaaaaat
     3661 cgttacgata ttccatcgtc cgccggatgg cggatatgga atcgtcgtaa ctgctacaaa
     3721 aacaacgatt ccatatcctc tcgctccttc gtcgctttcg gacgatggaa tattgttttt
     3781 gcataaaatc tcattttaaa acttcattat tattgccatt tatccgtaca tttgttctta
     3841 acaaaaatgt gattatgaaa ccgcaggatt acggatacag cgaaaaacac gagcaattca
     3901 gaaaagagaa caacctgact gaattcagcg tggcacgcgt gattgcggaa cacaaggagc
     3961 gttacattat tgtgtcggat cagggcgaat gcgaagctga aatcacaggc aatatgcgct
     4021 tttcggccgg cggccgcgaa aactttcctg cggtcggcga ttgggttgca gctaccattt
     4081 acgattccgg aatggcgctg attcataaaa tattcccacg ctcttcggtc attcaacgtc
     4141 aggcgattgg cgctgccggc gatattcaga ttattgctgc caatgtcgat tttgcttttc
     4201 tggtgcaggg tgccgatcgc gatttcaaca tcaaccggat tgagcgttat cttactatct
     4261 gctataactc gcgtatagag ccaattgtag tgattacaaa aatcgatttg tttgaagaaa
     4321 atcatatttc ggaattgctg aacagtatcc gtcaacgatt gcctggaatt ctggtattgg
     4381 ctatcagcag cgaaacacag cagggctacg atgagctgaa ttcgtttttt ctgaaaggca
     4441 aaacctattg catgctcggc tcctcgggtg ccggaaaatc aacattactc aatcatctta
     4501 ttggcgctga aacaatgaaa acccgctcgc tgagcgacag tacaagcaaa ggccgccaca
     4561 caaccacgca ccgcgaaatg tttgtgctgc ctaacggtgc gctgatgatt gacaatccag
     4621 gcatgaaaga agttggtatt gccaacgcgt cggccggact ggaagccact tttgatgaaa
     4681 ttgtccaatt ggctaagcaa tgcaggttca aggattgcac ccacactagc gagaatggat
     4741 gcgccgttcg tgctgctgtt gacagcggcg acatcgatcc ggcttcgctg gagaattttc
     4801 tgaaactcga aaaagaaaaa gagcattacg aatcaaccgt agcagagcgg aaacagaaag
     4861 acagagcttt cggcaaaatg ctgaaaaatt ataaaaaggg aaaatacaag gacaattttt
     4921 aattaccttt acttttcaaa acccattcat catgacctcc accatcagca tctgcggact
     4981 cgattgcaac acctgtcccg agtacggaaa acaatgtcag ggctgtgttg ccagtcaggg
     5041 aaaaattttc tgggctgttc attacaacat ggataaatgt ccgctgcaca aatgctgcac
     5101 agatgaacac aactacgctc actgcggtca gtgcgatcag cttccgtgca aaacctattt
     5161 cgatatgcgc gatccttcat ataccgaaga acagcatctg gagggcatta aaacacgagt
     5221 gaaaacacta agaaaattga actaatttac ggcagctccg cccgatccag aaaaacaaat
     5281 ccattcagat gatcaatttc gtgttgaaaa atatgcgcgg tgtattcgtg tgaaatcctc
     5341 tcgctgcagc gtattccatc tggagtgtca taaacaacat caacccaaat ggcacgccat
     5401 gatgagccgg aaactccggg aatactgaga cagccgtcag accttaattt tacggtatcg
     5461 gaataagcag taatgtatga attcaaataa cattcgaaag gagctccggt tttgtcgtat
     5521 cgttttaccc agataacacg tttattcact cccacctgcg gagcagcaag tccaacaccc
     5581 ggattggccg gattggttac ggtcttgtac atgcgatcct tcaacatttt cagataaaat
     5641 gatccgaatt catttacatt tccactatta ttacgcagaa tcagagagtc ctcgtaaatg
     5701 aaataattaa gtacccgcat cttttcggta gcagagcccg aagcaatcaa atttacttct
     5761 tcataagaaa agtcggcagg gtgatctcca tggtcgtaac acgaaaccaa aacagcacca
     5821 gtaattacaa gtgcgaaaat caaagaaaat aatttcatcc tattttaatt tacaacaagc
     5881 gttcttctgc aaaagtaata aaaattcatt gagaatcatg gcagtagctc cccagataac
     5941 atgattatcc gcacgaaaac aaggcgccgt ccctgtcaaa tgacctgatt tataatgaaa
     6001 aacgcctctg gattcaggat tggccagcca gagcacatct gcatcaaaga ctgcactaac
     6061 ctcgtgtaaa tcaggcatat actttggttt tttatcaaca aaacccacaa aaggatgaat
     6121 aagaaatcgt gaaagcggca catagagatc ggataattga ccgatataag aaacaggaaa
     6181 gtgaatcccg gtttcctcca gtgtttcccg cacggcagtc tgccagaaat taacatcttc
     6241 gggctcagat ctgcctcccg gaaaagctat ttgcccgctg tgaacaccgt catattcggc
     6301 tcttttaata aaaacgatac gggttgaatt caaatgattg aacaacagca gaagaacagc
     6361 actgtgggaa aagggctctg attcggaaaa cattgtcata gagcgacctt caggcatcat
     6421 ttcggcatga gcaatactgc cgggtaaggg atgattttgg aaaagtttag caataaaatc
     6481 acgcgaaaac atgcataaaa gtaaaatatt ttttgttaca tcgggaaaaa tatatacttt
     6541 tgcgatgaaa accaaatttc aattactaac caaaaccaaa aaacaatgaa aaagattttc
     6601 agcgttctcg cagcagcagt tattgctttc gcattcacaa gttgtggtgg tggtgccgac
     6661 actcctgaaa aagtggccga aaaattcctc cagcacattg ctaaaagcga attcaaagat
     6721 gcaaaagaac ttgctaccgg cgatgcagta aaaacaatcg aaactctcga aagctttgct
     6781 tcaatgagcg aaatgggcgg cgaaaaaccc gaagctaaag aagtaaaaat cgaaaacatg
     6841 aaatgcgaca ccaaagaaga tgcagctgct tgtacctaca aacaagacgg taaagatggc
     6901 aacatcgatc tgaaaaaagt tgacggcgca tggaaagttt caagcttccc gaaagaaacc
     6961 accggtggcg atgaaaccga agaagttgac atggaaagcg acacaactgc tactgatgat
     7021 gtaactgaag aagttgctga ataagtttta gcttatctta catatagcag caatgaagaa
     7081 gggacttcta acaatcctga tattcattgc tgcttttgtt ttgtttctgt caacctatga
     7141 tttacggagc gaacaaaagc agcaatatga atcctacgac ctgacacctg acgaatacgc
     7201 gatgctgaaa gacggggatt tgattttacg tcatggctat ggactcgtca gcgatgcaat
     7261 tgccaattca atgaacgaag aaaatcatgt ttcgcatgtg gccgtacttg tgaaaagcga
     7321 taccgccaaa aacggtttcc gcatcgttca ttgtgtttct caatcattgt cgccctggga
     7381 tggagtccag gagcagactt tcccgcgatt tatccacgac agccagcgca attccattat
     7441 ggtggtgcgt tttaaaggtg ccaacgactc cacaagacac aaaatcagcg aaaaggcgct
     7501 tgattatctc aatcagcgag tcacgtttga ccatgctttc gatattgcag actcatcaac
     7561 attctattgt tcggaactgc cttacatgat tttcaaaaac gaattcggcg tcgatatttt
     7621 taaggacaaa atgaatgagc agcaggatca catgaaattc tccaatttct ggaatccaga
     7681 caatttcgag gtcatcatta atcacaatac caagcggtaa tcatttccga aggaattttc
     7741 cttcgacgga tctgcaagtt tctgactggg aatacaacat ccaacgtccc atagggacga
     7801 aatgttttta tgcattggct attgcgtacc tgacagcatg aactcgcctt ttttcttgct
     7861 accaatatgt ttacaacgaa cataacaaga tacattatac caaaataaaa tatataacag
     7921 tctgatttca atggatgact ccggaggagt ctcatgtctg tagaaatgat ataaataaac
     7981 tacgactcca gcggagtcga atgtatt
//
LOCUS       DAOPKE010000118         7995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9593_length_7995_cov_15.560579, whole genome shotgun sequence.
ACCESSION   DAOPKE010000118 DAOPKE010000000
VERSION     DAOPKE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9593_length_7995_cov_15.560579"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..370)
                     /locus_tag="PKY63_11460"
     CDS             complement(95..370)
                     /locus_tag="PKY63_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01277.1"
                     /translation="MKKIAHPTFWRPQNFYSAMGARLLTRDNALPRSLFTDTEFRQLF
                     DNLLGALDYFSPTSALQSDKIPRQQCCNISLFWLTVECRGNVAGVSL"
     gene            476..2692
                     /locus_tag="PKY63_11465"
     CDS             476..2692
                     /locus_tag="PKY63_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HPF01278.1"
                     /translation="MNWKFWKKIRLKRWHKIVGIIILLFIILLAFLPAIAKWYITTHG
                     EDLIGRKIDMSGLHINYFRCSVDIEEFVLYEKNGSDKFVSFDELYVNFAPWHLFSNEY
                     AFTEITLANPYVSIIYSEDGFNFDDLTESSDTTETEEPEDTTSDPTKYLIRNLNLIGG
                     YIRYEDKTTNSVTEVKQLGITVPEIAWNNSKSELGIDFILGEKGEVSLGGNVNQAAGT
                     YKVQAKTKNIDISPFAGYLKDYMAVSSMTGLLYTDVTVNGEMAEPMNVVVSGEAGIKD
                     FALNDMDGNHFFSAADMYIKMDSLDIGKENYCINTVRVESPKLTAVLDKTGTNFDRVL
                     EPYFSDTLDVAEDTTVTDTSTTEMHYSIEHLEINDCAVAFSDLTLNRPFRFDITNLDF
                     KMEDYSDLATAVKMDFSMMLNNTGSFKGNAVVDMVTMESMTFDGVIANLDMVSLSPYS
                     EYFIARPITKGTFNYNVKLTMNPTYLNNQNSLKIVNLETGKKTKDTTAYKAPIGLALY
                     ILKDRNNVIGFDLPVTGNPSDPSFKLGKIIWKTLEEFLIKTATAPFKAIGNLFGANPE
                     SIRQVPFEIMQDSLLNDQKNNLDKIAEIIIKKPELIFSFSQSTNPDEEMKLLAIHEAK
                     LQFVRSQSGDGADPLKIQEEVNKLRDNDPEFRQWLGMTELMGAAQVPGFCMQKVGTER
                     ASSLLNELMLKRENLLRSYFAEKGVPAYSIQFKMADLRNLPEDLKKPMFTVEITMN"
     gene            2703..3134
                     /locus_tag="PKY63_11470"
     CDS             2703..3134
                     /locus_tag="PKY63_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPF01279.1"
                     /translation="MRIWSVHPEYLDVKGLVAVWRETLLARHVLLGLTKGYTNHPQLD
                     RFKAAADPVNSIDYYLSVVYDEALKRGYHFNKTKFNKVSKPKKISVTNGQLQYETKHL
                     LNKLKLRDPAKYKLLQHEKQMKPHPLFDVIEGGIEKWEIVL"
     gene            3299..4630
                     /locus_tag="PKY63_11475"
     CDS             3299..4630
                     /locus_tag="PKY63_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01280.1"
                     /translation="MSQDLSVNNKKHKWLKWLIFLMFSAIVLFLSFNRHSKSGQFNYH
                     SEIWADKAGYYVYLPALFNYGFNASAFPDSIAAKTGDGFRIDTANNKVITKYSCGVAI
                     LQMPFFLAAGIFDDLSDPGRIPGFTKAHHAAINIAAVFYLLLGFLFLGRYLENRFHKP
                     LVWLVVFVLFTATNLYYYSVDETGMSHVYSFFLFSLFLFLLQKTSYLQKAGAGILLIF
                     GIVAGLIVLIRPTGLLFFFAAFLLEIQNVHEFKFRIKSLFQPKIMLWFIAGVVLIFLP
                     QLLYLQYTFGSPFHYTYESEGFYWTNPQLLKTWFSTNNGLFLYTPFFLIIVIACIYAA
                     FKRKADGLKMLLLFVIISYVFSSWWMWYFGCAFGARSYVEYLAIFSIPLTFLFLKIHR
                     SGIVAIISFYLAVVSCIVLNFMMTYSWDGCYYGSGDWDWDAWIGTVISKLR"
     gene            5258..6244
                     /locus_tag="PKY63_11480"
     CDS             5258..6244
                     /locus_tag="PKY63_11480"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPF01281.1"
                     /translation="MKLCIVGITGLVGSELIQVLNESPLHVDEFICAASERSKGKEVE
                     IRGKKNTVVTPAMACVMKPDIAIFSAGSGVSKEWAPRFAANGCFVIDNSSQWRMYDEI
                     PLVVPEINADTITRETKIIANPNCSTIQLVLALSRLHAAFGIKRIVVSTYQSVSGTGM
                     YAVEQLMNERAGKQGNMVYPHPIDMNCLPHGGSFLPNGYTTEEEKLVNETRKILRAPQ
                     IQVSATVVRVPVTGGHSESVNVEFERPYEIADIYKLLSETPGVTVQDNPAENLYPMPL
                     YAKGKNDTFVGRIRRDDSIANGLNLWVVADNLRKGAATNAVQIAEWLGGKML"
     gene            complement(6279..6878)
                     /locus_tag="PKY63_11485"
     CDS             complement(6279..6878)
                     /locus_tag="PKY63_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF01282.1"
                     /translation="MENKYQYIDPDYAYTNKNGVLRNLANIEDERILLAYESLKVSKR
                     VEELFENPITIVDADSLLIIHYYLFQDVYEWAGKVRTVNISKNGKPFFDKERFNIAFQ
                     YINTLISNYRAIRKSMKQELSQSLAEILDHVNYLHPFREGNGRTQREFIRLLALEKNM
                     ILNLNPPDNKSVYDRYMLGTINSDVKTLTELILEQIMNQ"
     gene            complement(6868..7089)
                     /locus_tag="PKY63_11490"
     CDS             complement(6868..7089)
                     /locus_tag="PKY63_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HPF01283.1"
                     /translation="MYKTLDIDRKNLTLMGVKFSDLKTLESTANAIGTNMFEGFEPTR
                     ELIQLYVDWKNGCISETDFLAKLYAIYGK"
     gene            complement(7410..7916)
                     /locus_tag="PKY63_11495"
     CDS             complement(7410..7916)
                     /locus_tag="PKY63_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01284.1"
                     /translation="MNSKRLTYQERAERLAKTIDIAEKVIAVSKSLGEKTRTHLLKWG
                     REIKHMALNPEPQFKKVASLKYLENDFLIYWNEADGEDIEKFWIELYKNGIDFERKDN
                     FQSVLKRKRIKDIHEYDSVVDNVVAAKQIGQIDKNQVKELNQLIGEFEQRQTKKLNAN
                     KKINASRL"
BASE COUNT         2211 a         1762 c         1701 g         2321 t
ORIGIN      
        1 ccggcgcgga gaccgggaac ttcattagac ttttttgttg attttcagaa ttttacgcac
       61 ttgtatcatt agcagtgtcg ctaatgaaga gcttttacag agaaacgcca gcgacgtttc
      121 cccgacattc taccgtcagc cagaacaagc tgatgttgca acattgctga cgtggaattt
      181 tatctgactg caaggcagac gtcggggaga aataatccaa agcgcccaaa agattgtcaa
      241 acaactggcg gaattccgtg tccgtaaata agcttctggg taaagcattg tcacgcgtca
      301 ggagacgcgc gcccattgct gagtagaaat tctgcggacg ccaaaatgtt gggtgcgcga
      361 tttttttcaa tgtttgaaaa tttccgacct ttacaaaccg tagaagaaat tcttctcgct
      421 gaaatatttg tattttagct ttttgagagt ttgatcattt tatataaaaa atattatgaa
      481 ctggaaattc tggaaaaaaa tccgattaaa aagatggcat aaaatcgttg gtattattat
      541 tctgcttttc attattctgc tggcatttct gccagctatt gccaaatggt acatcaccac
      601 acacggcgaa gacctgatcg gacgtaaaat cgacatgagc ggacttcata tcaactattt
      661 ccggtgcagc gttgatatcg aagaatttgt cctgtacgaa aaaaacggca gtgacaaatt
      721 tgtaagtttc gatgagctct acgtcaattt cgcaccatgg catctgttca gcaatgaata
      781 tgcttttacc gaaatcactt tggccaatcc atatgtttca atcatctatt cggaagatgg
      841 ttttaatttc gatgacctga ccgaatcgag cgacaccaca gaaacagaag aaccagaaga
      901 caccacttcg gatccgacaa aatatctcat ccgcaatctg aatctcattg gtggctatat
      961 ccggtacgaa gacaaaacaa ccaacagcgt taccgaagtt aagcagctcg gaatcacggt
     1021 tcctgaaatt gcatggaaca acagcaaatc ggaactgggc atcgatttca ttcttggcga
     1081 aaaaggcgaa gtatcgctgg gaggcaatgt taatcaggcc gccggcactt acaaagttca
     1141 ggcaaaaaca aaaaatatcg acatttctcc atttgcgggc tatctgaaag actacatggc
     1201 ggtgagctcc atgaccggac ttctctacac cgatgtgact gtcaacggcg aaatggccga
     1261 gcccatgaat gtggttgttt caggcgaagc aggaataaag gactttgccc tgaatgacat
     1321 ggatggcaat catttctttt ctgccgccga catgtacatc aaaatggatt cgctcgacat
     1381 cggcaaagaa aattattgta tcaataccgt tcgtgttgaa tcaccaaaac ttaccgcagt
     1441 tcttgataag acaggaacca atttcgaccg cgtgctcgaa ccctattttt ccgacacgct
     1501 tgatgtggcc gaagatacaa cagtgactga cacttcgaca acagaaatgc attactccat
     1561 cgaacatctc gaaatcaatg actgcgcggt tgccttttct gacctgacgc ttaatcgtcc
     1621 tttccgtttt gatatcacca atcttgattt caaaatggaa gattacagtg atctggcaac
     1681 cgctgtgaaa atggatttca gcatgatgct caacaatacc ggttcgttca agggcaatgc
     1741 agttgttgat atggtcacta tggaatccat gacattcgac ggcgtcattg caaacctcga
     1801 tatggtaagt ctgtcgccct attccgaata tttcattgca agaccgatta cgaaaggaac
     1861 gttcaattac aacgtgaaac tcaccatgaa tccgacgtat ctgaacaatc agaattcgct
     1921 gaaaattgtt aatctggaaa caggaaagaa aacaaaagac accaccgcat acaaagcccc
     1981 gatcggtctt gcactctaca ttctgaaaga ccgcaacaac gttatcggat ttgatttgcc
     2041 ggtcaccgga aatccatcag atccatcatt caagcttgga aaaatcatct ggaaaacact
     2101 cgaggaattt ttgatcaaaa ccgccacagc gccattcaag gccatcggaa atctctttgg
     2161 tgccaatccc gaaagcatcc gccaggttcc gtttgaaatc atgcaggaca gtttgctgaa
     2221 tgatcagaaa aacaatctgg ataaaattgc tgagatcatt atcaaaaaac cggaactcat
     2281 tttctcgttt tcacaatcta ccaatcccga tgaggaaatg aaactgcttg ccattcacga
     2341 agcgaaactg cagtttgttc gcagccagtc gggcgatggt gccgatccgc tcaaaattca
     2401 ggaagaagta aataaactgc gcgacaatga tccggaattc cgccagtggc tcggaatgac
     2461 cgaactcatg ggcgccgcac aggttcccgg tttctgcatg cagaaggtag gaactgaacg
     2521 tgcatcctct ttgctgaacg aattgatgct gaagcgtgaa aacctcttgc ggagctattt
     2581 tgcagagaaa ggcgttccgg catattccat acaattcaaa atggccgacc tccgcaatct
     2641 gcccgaagat ctgaaaaaac caatgtttac ggttgagata accatgaatt aaaacatctc
     2701 caatgcgcat ctggtcagta catcccgaat atctcgacgt caaaggcctc gtggctgtgt
     2761 ggcgcgagac gctgctggcg cggcatgtgc tgctgggtct taccaaagga tacaccaatc
     2821 atccgcagct cgaccgtttc aaagccgcag cagatccggt aaacagcatc gactattatt
     2881 tgtctgtggt ctatgatgaa gcgctgaaac gtggttatca tttcaacaaa acaaaattca
     2941 acaaggtcag caagccgaaa aaaatcagcg ttaccaacgg acagctgcaa tacgaaacga
     3001 aacatctgct gaacaagctc aaactgcgcg atccggccaa atacaagctg ctgcaacacg
     3061 aaaaacaaat gaagccgcac ccgctgtttg atgtgattga gggcgggatt gagaaatggg
     3121 aaatcgtttt gtagtggtca gtagccagtg gtcagtggtc agttatgagt tataagttat
     3181 gagttatgag ttatgagggc ttagcgtttt ctgagaactg agggcggcat gagctcctga
     3241 ggaatttttc aattcaaatt gcaaacagac ttttgatata tttgcagcaa acaaatgtat
     3301 gtcgcaggat ttatccgtca acaataaaaa gcacaaatgg ctgaaatggc tgattttcct
     3361 gatgttttca gctatcgttc tgtttctttc ctttaaccgg cacagtaagt cgggacaatt
     3421 caattatcac agcgaaatct gggctgataa agccggctat tatgtgtatc tgcctgcttt
     3481 gttcaattac ggattcaatg cttcagcttt tcccgattca atcgcagcaa agaccggaga
     3541 cggattccgc atcgacacgg caaacaataa agtgattacg aaatattcat gcggtgtagc
     3601 catactgcaa atgccgtttt ttctggcggc cggtattttc gatgacttat cggatccggg
     3661 ccggattccg ggatttacga aggcacacca tgctgccata aacattgccg ccgtctttta
     3721 tctgttgctg gggttccttt ttcttggccg ctatcttgaa aaccgttttc acaagccact
     3781 tgtatggttg gtggtgtttg ttttgtttac tgcaacaaat ctctattatt actcggttga
     3841 tgaaaccggt atgtcgcatg tttactcgtt ttttctgttc agtctttttc tttttctgct
     3901 tcagaaaacg tcttatttac aaaaggccgg tgcaggaatt ctattgatat tcgggattgt
     3961 ggccggtttg attgtactga taagacctac aggattgttg ttcttttttg ccgcatttct
     4021 gctggagatt caaaatgtac atgagttcaa attccgcatc aaatcactat ttcagcccaa
     4081 aattatgctc tggttcattg ccggagtcgt gctgattttt ctgccacagt tactttatct
     4141 gcaatacaca tttggttcgc cttttcatta tacctacgaa agcgaaggct tttactggac
     4201 aaatccgcaa ttacttaaaa cctggttttc gaccaacaac ggattgtttt tatacacgcc
     4261 gttttttctg attatcgtga ttgcatgtat atatgcggca ttcaaacgca aggccgatgg
     4321 actgaagatg cttttgctgt ttgtcattat ttcttacgta ttttcttcgt ggtggatgtg
     4381 gtatttcggc tgtgcctttg gcgcacggag ctatgtggaa tatctggcca tattcagtat
     4441 tcctctcact tttctttttc tgaaaatcca cagatcaggc attgttgcaa tcatttcatt
     4501 ttatttggcg gtagtgtcgt gtattgtcct gaatttcatg atgacctaca gctgggatgg
     4561 ttgctattat ggatccggtg actgggactg ggatgcatgg attggaactg ttatttcaaa
     4621 attacgctga ccaaaaaccg cttcagaagc tgcaagtgca gatagaagct ttgcctacag
     4681 cagtgaacgg tgagtggtga gcggtgaacg aaatttcact ggttactaac ggggtctgca
     4741 agtcttaaac gctctcaggt cgctttacgt gaaatcacgt aaaacgagac aagctgtgac
     4801 acaggcgcga cgctgagaga ttaaagcagc aaaagttcac tgctcgtacc gacgctgcgg
     4861 tcggcacttg cagctggtca cagctcacta cttactccag actccttctc aaagctgacg
     4921 gctgatccgc cgcggcggac cgacagctga tagcttgaga attatgagtg aaaattttac
     4981 tgtctactga ctccgccgcg gcggatcaaa actggctact atcttatgtc ttgcttgctc
     5041 gtccccgcag ccctgcgctt cgcaagctca gctttcaggg catcgggcta ttaatattgg
     5101 actacgtccg tagtcccttt caaaggaacc aactatcgta atttttgggg taaacgtttg
     5161 tagccacgtc ataaaactgg ccattaacta ctcacaactg actacttctt cgtacttttg
     5221 caaacgattt tgcgttattg aaaaaaatga ttgaaaaatg aaactctgta ttgtcggtat
     5281 taccggtctg gtgggaagcg agctgattca ggtgctgaac gaaagcccgc tgcatgtgga
     5341 tgaatttatc tgcgctgctt cggaacgttc caaaggaaag gaagttgaaa tccgtgggaa
     5401 aaaaaataca gttgtaactc ctgccatggc ttgtgtcatg aagcccgata tcgccatatt
     5461 ttctgcgggc tcgggtgtat ccaaagaatg ggcgccacgc tttgctgcca acggatgttt
     5521 tgtgattgac aatagttcgc agtggcgcat gtatgacgaa atcccgctgg tggtgcccga
     5581 aatcaatgcc gacaccatta cacgtgaaac aaaaatcatt gccaatccaa attgctccac
     5641 cattcagctg gtgcttgctc tgagccgtct gcatgcggct ttcggcatca aacgcattgt
     5701 ggtttcgacg tatcagagtg taagcggaac gggtatgtat gccgttgaac aactcatgaa
     5761 cgaacgcgcc ggaaagcaag gcaacatggt ttatccgcat ccgatcgaca tgaactgtct
     5821 gccgcacggc ggatcgtttc tgcccaatgg ctataccacc gaagaagaaa aattagtgaa
     5881 cgaaacacgt aaaattctgc gtgccccgca gattcaggta tcggccaccg tggtgcgtgt
     5941 gcctgtaacc ggcggccaca gcgaatcggt gaatgttgag tttgaaagac cctatgaaat
     6001 cgccgatatt tacaagctgc tgagcgaaac tcccggcgtt acggtgcagg acaatcccgc
     6061 cgaaaacctg tatccgatgc cgctctacgc caaaggaaag aacgacacct ttgtaggccg
     6121 catccgccgc gacgacagca ttgccaacgg actcaacctg tgggttgtcg ccgataacct
     6181 ccgcaaaggc gccgccacca atgcggtgca gattgctgag tggttgggtg gaaaaatgct
     6241 gtgaatttgt aaaaaccttt gcttgctgtt ggctggttct attggttcat tatctgttct
     6301 aaaatcaatt ctgtcaatgt ttttacatca ctatttatcg taccaagcat ataccggtca
     6361 tatacgcttt tattgtcggg tgggttgaga tttaaaatca tgtttttttc taatgccagc
     6421 aatcttataa attctctttg tgttcgccca ttaccttctc tgaaaggatg taaataattt
     6481 acatgatcca atatttcggc taatgattga gacagctctt gtttcattga ttttcgaatg
     6541 gctctgtaat ttgaaataag ggtgtttatg tattgaaaag caatattaaa gcgttctttg
     6601 tcaaaaaaag gttttccgtt tttactgatg ttaaccgttc tgacttttcc agcccattca
     6661 tatacatcct gaaacaaata ataatgaatg atcagcaatg aatcagcgtc tacaattgtg
     6721 attggatttt caaataattc ctcaactctt ttagaaacct ttaagctctc atacgccagc
     6781 aatattcttt catcttcaat attggccaaa ttacgaagca ccccgttttt attggtgtat
     6841 gcgtaatccg ggtcaatgta ctgatattta ttttccataa atggcatata gcttcgccag
     6901 aaagtctgtt tctgaaatac agccattttt ccaatctaca tacaactgta tcaattccct
     6961 ggttggttca aaaccctcaa acatatttgt gccaatagca tttgccgtac tttccaatgt
     7021 ttttaggtca gaaaatttca cacccattaa tgtcaggttt tttcggtcta tgtctaatgt
     7081 tttatacatt gttgttcaat tcagaaacaa ggcaaatata cgggtttttc agcacttttt
     7141 caagcttttg aagcactttt tcagcgaaac aggcacaaaa tccatgttgt caacacaaat
     7201 gtattactta caaaaacacc aatggaaata ttttattatg cgattgaaaa ttttttatgt
     7261 ggtgcaaatc gctttgtggg tggtggaaaa gtgccaaaat gggggtattt cgtgcaacgg
     7321 tttgaagata cgcagggcgg cttcgtccgt ggtgttgtaa agaaacgaaa gatatctctg
     7381 tccaccgtaa agcccctacc gtatgggtat taaagccgac ttgcgtttat ttttttgttg
     7441 gcgttaagtt tttttgtctg tcgttgctca aactccccga ttaattgatt caattcttta
     7501 acttgattct tatcaatttg tccgatttgc ttagcagcaa caacattgtc aactacgctg
     7561 tcgtattcgt gaatgtcttt aattctcttt cttttcaaaa cggattgaaa attgtctttt
     7621 cgttcaaagt caatcccatt cttatataat tcaatccaga acttctcgat atcttctcca
     7681 tctgcctcat tccaatagat taagaagtca ttctctagat atttaagact ggctactttc
     7741 ttaaattgag gttccgggtt aagagccata tgtttaattt ctcggcccca ttttaataag
     7801 tgagttctgg ttttctcgcc taatgacttt gagacagcaa tcaccttttc ggcaatgtcg
     7861 atcgtctttg caagccgttc agctctttct tgatatgtca gtctttttga attcattttt
     7921 acatacacgc caacatcttg cacccaccac acaaatatag cacttacaaa aacaccattg
     7981 aaaagatttt attat
//
LOCUS       DAOPKE010000119         7924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_9710_length_7924_cov_10.672512, whole genome shotgun sequence.
ACCESSION   DAOPKE010000119 DAOPKE010000000
VERSION     DAOPKE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9710_length_7924_cov_10.672512"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..311)
                     /locus_tag="PKY63_11500"
     CDS             complement(84..311)
                     /locus_tag="PKY63_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01285.1"
                     /translation="MKIYSAYRKTSATERNTLKNEIVVNWQRVDDKKKARNKRESREE
                     PARDSWASLVARRIQVMSEKLSGFFYPEVVE"
     gene            469..1602
                     /locus_tag="PKY63_11505"
     CDS             469..1602
                     /locus_tag="PKY63_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01286.1"
                     /translation="MKFSFSSGPLSLILDQLSGLYPTATQFIALENNGLASFNVRFYN
                     SDSQELSDGGSRNDIPDLNKLFGLRNNHFVWQNENDLPFSWTQVRQSNNVDMFRELER
                     NVLVIRVNDARWAGFILVYFSKNKKHFGFLDSESSFSHENREIIGQTAVNAIQLCLKN
                     AVRDSQVLDSIQKSMQALTGNRKMEDRGNQNQVKLLENMIVEMAVEYLHNLGREQKIT
                     IALSPDARDKIQSFRGSPASLRSMLHQASVLAVNLGAGNPVIIESWHLISGAAEYEIE
                     TSKSVPVIQHRHMKIYQWLDRVEEAVKTVISGHENPTGAIVGKAMQPAVSAPAISDML
                     NKQKQKILVLLNEFPDRWPQTRNHFKPLQNLLVRKDFDESKSA"
     gene            complement(1604..2758)
                     /locus_tag="PKY63_11510"
     CDS             complement(1604..2758)
                     /locus_tag="PKY63_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01287.1"
                     /translation="MLNRQYTETIESRIINNPVVAILGPRQCGKTTLAKLIMANHPDS
                     VYIDLEKPSDLSKLDNAEWFFSANRGKLICIDEIQRKPELFALLRSIVDETNENGQFL
                     VLGSASRDLIRQSSESLAGRISYFTLSPLLVNEIAKDTSIEKYLTRGGFPRSFLASSD
                     EYSFQWRKDFITTFIERDILQFKGVVPAAIRRLMTMIAHVNGQTINYSSIGNSLGISN
                     VALKNNIDLLSGSFILNIVPQVNRNTGKRLVKAPKIYFSDPGIVEALLNLRNFNSIVS
                     HPAFGSLWETLVLMNLSGNFPDAEISFYRTSNGAELDFIVMDNDTTVCIECKTNSNPV
                     LTKGNFISIEDLKPVKTFVVSPVTNSYEYAENIIVTPLSALPGLIREQLS"
     gene            complement(2935..3150)
                     /locus_tag="PKY63_11515"
     CDS             complement(2935..3150)
                     /locus_tag="PKY63_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01288.1"
                     /translation="MARKKKRYRSIKLGISTQKAEKFAQFCASKGMTPNLYLRKVIDV
                     NLAGFKGYEKKEQKNPRQLGIFDFIEP"
     gene            complement(3831..5108)
                     /locus_tag="PKY63_11520"
     CDS             complement(3831..5108)
                     /locus_tag="PKY63_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01289.1"
                     /translation="MAECKEFADYLVTECGIKISDFTLARLMGVVVSKSSPSKYTLDS
                     LSEYCGYTDWHSFVNSSHEKIIEGRNNILSPSDNSFSGVSSSELMLLQSCLENKAYVP
                     VIEYLYKMAPVIIENIAKTGDFALVRLLAGAIGKSEDARNALIPAIARENYLRNLYFN
                     NMVDIEGIVVYYGDMVEKYYLKNIRPTSDSFMENDIWAWCMIMYKHLYAQNLKEFLKT
                     GYMLFKRYNADEITNESIPNYYPVARFHACQIMYKFFSSPCTPNSWYEKKLQFILKEV
                     DSIPEARQVIFIPQIAEALYVAGQKELIFAFTPYFDKFIRNYFADGTFTFESDSLHKL
                     LFYLHLALDESGTFEKNSVQFKNFIGKEIECPIMPDGFHLADYMTLVVHSMYEYKPEA
                     SRDFLEHARKIAVKLKNKHFEKIAQRFIARSGE"
     gene            5404..7671
                     /locus_tag="PKY63_11525"
     CDS             5404..7671
                     /locus_tag="PKY63_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01290.1"
                     /translation="MKVFYLLVFLVLAFGYLCAQDTIIQLKPVEISTNKDEITRLAPH
                     YELINLEEIQLQTMSKEIPAILSQLPGIHYVPGRGIGDTELRIRGFDVSNIQLLINDI
                     PESYVDEGWTYWSNFTGIEDFTQSVFVTKGVSNTHPFSKAGGTISIHLLEASAARFTR
                     FSSSYSQLSGNKINFTHSSGLLKNGFAWAVGGTYRFGSSYKDETWFRGGNYFFNASKK
                     LNTKHTLQLTVFGAPQQHGQASNYLPDSVLQDKELSYNPSWGYLNGDPKQSNINFYHR
                     TLVQLKHLAEFSKKNHLKSILNFSNGSGGATVNFGNWLPGIEQIDFDAAYSANQNNYD
                     STFGGNNALYFLGDYVHKEASFSLYSELNHHFSDSARIDAGILAYSSLFIKDNGRIND
                     LLGADFQVYPGETSVRHEGDDIWYKNKASRHGAGVFATWVKSADNTDFMLSGSFIRST
                     GSYTELFNVPDSAIERTDMKWFNSYNLKGSVQHYLTKNLAVYTTAGFQHRRPPVLFIG
                     KTVIDNVIPENFTTVEAGMRRYFESPFQGQIHGSVYYTARRNQNFVNYYTDGLSGEMF
                     TFSVEGLNSRYMGAEIEFEARWSEKFMGSVAVSYNNVMWLNNIQSTVTDLSGAPIDTI
                     VLNAKGIFDGNQPQLAANISGLYTIRSHYQIFAEALYNGLNYSWFNVLNYTGSNEVKQ
                     SWQIPDYVIFDLGIAYKTKLRNKVDMRLSATIHNVFDNHFIAEGIDGANHDKNSSYVL
                     YGHGRCLTAGVVLEF"
BASE COUNT         2320 a         1666 c         1549 g         2389 t
ORIGIN      
        1 agtgaaaaat taaatttttc ccaattattt ggccattaac acagaatctc gaaggttttt
       61 gtatcccttc gggggatttt tgtttactcc accacctccg gatagaaaaa tccagagagc
      121 ttttcgctca ttacctgtat gcgccgggcc accaagcttg cccatgaatc gcgcgccggc
      181 tcttccctgc tctctcgctt attacgggcc ttttttttgt cgtcaacgcg ctgccagttg
      241 acaacaattt cattctttaa agtatttcgc tccgtagcgg atgtttttct gtatgcactg
      301 tatattttca tggctgaatt ttttctgtaa agatatgatg cgtaaaatac tgaaaacaaa
      361 catattaagc attttaagtg cttaataaat taattaatgc gcttaataag cacttaattt
      421 tatttgcttt tgtaaaataa agtgcttaat tttgtggtta atattcttat gaaattcagc
      481 ttttccagcg gtcccttgtc cttgattttg gatcagctgt caggtcttta cccgacagct
      541 actcagttta ttgcgctgga aaacaatgga ttagcatcct tcaatgtcag gttttacaac
      601 agcgattcgc aggaactgag cgatggtggc tcccgaaacg atattcctga tctgaacaaa
      661 ctcttcggac tgcgcaacaa ccacttcgtg tggcagaacg aaaatgacct gcccttttca
      721 tggacacaag tacgccagag caacaatgtg gatatgtttc gcgagctcga acggaacgta
      781 ctggtcattc gggtgaatga tgcccgctgg gctggtttta ttctggtcta tttcagcaag
      841 aacaaaaaac actttggctt tctcgattca gagtcgagct tcagtcatga aaaccgcgaa
      901 atcatcgggc agaccgctgt caatgccatt cagctctgtc tgaaaaatgc cgtacgcgac
      961 agccaggtgc tcgacagtat tcagaaaagc atgcaggcac tcactggtaa ccgcaaaatg
     1021 gaagaccgtg gtaatcagaa tcaggtgaaa ctgcttgaaa acatgattgt tgaaatggcc
     1081 gttgagtatc tgcacaacct cggtcgtgaa cagaaaatca ccattgccct cagtcctgat
     1141 gccagggata aaatacaatc tttccgcgga agtccggcca gtctgcgatc aatgctacat
     1201 caggcaagtg ttctggcggt caatctgggc gccggcaatc cggtcatcat tgaatcgtgg
     1261 catctgatat cgggcgctgc cgaatacgaa atagaaacat caaaatcggt tccggtgatt
     1321 caacaccggc atatgaaaat ttatcagtgg ctcgaccgag tcgaagaagc cgtgaaaaca
     1381 gtgatttcag gacacgaaaa tccaaccgga gccatcgtgg gcaaagccat gcagccggct
     1441 gtgtcggccc ccgccatcag cgatatgctg aataagcaaa agcaaaaaat tctggtgttg
     1501 cttaatgaat tccccgaccg ctggccgcaa acgcgtaatc atttcaaacc gcttcaaaat
     1561 ctgctggtcc gtaaggattt tgatgagagt aagagcgcgt aaatcagctc aattgctctc
     1621 taataagtcc tggtaatgca gaaagcgggg ttactataat gttttcagca tattcatagg
     1681 aattggtcac cggtgataca acaaaagttt ttacaggttt taggtcttca atacttataa
     1741 aattcccctt tgtcaataca ggattagaat ttgttttgca ctcaatacaa acagtagtat
     1801 cgttatccat gacaataaaa tccaattcag caccattcga tgttctgtaa aaactgattt
     1861 cagcatctgg aaaattcccg gacagattca tgagtaccag tgtttcccaa agtgacccaa
     1921 atgcagggtg cgaaactatt gaattgaaat tccggagatt tagcaaagct tccacaatcc
     1981 caggatcgct aaaataaatt ttaggtgctt taaccaacct tttacctgtg ttgcggttga
     2041 cctgtggcac tatattcaaa ataaacgatc cggataataa atcgatgttg tttttaagtg
     2101 ctacattgct tattcccagt gaatttccaa ttgatgaata atttattgtt tgcccgttta
     2161 cgtgtgcaat cattgtcatc aaccgcctga ttgcagcagg aacaactcct ttgaattgta
     2221 aaatatccct ttcaataaaa gtagtgatga agtcttttcg ccattgaaac gaatattcgt
     2281 cgcttgacgc cagaaagctc ctgggaaatc cacctcgggt cagatatttt tcaatgcttg
     2341 tatcctttgc gatttcattt acaagtaatg gtgacagcgt aaaataggaa attcttccgg
     2401 caaggctttc agagctttgt ctgattaaat cacgtgaagc tgatcctaaa acaagaaact
     2461 gtccattttc atttgtttca tcaacaatgc tccggagcaa tgcaaacagt tcaggcttcc
     2521 gctgtatttc atcaatgcag attagttttc ctctatttgc actaaaaaac cattcagcat
     2581 tgtcaagttt tgacaaatcc gaaggttttt ccagatctat gtaaacagaa tccggatgat
     2641 ttgccattat cagctttgca agagtggttt taccacattg gcgcggccca agaatagcaa
     2701 caacaggatt gttgattatt ctgctttcta ttgtttcagt gtattgtcta tttaacattt
     2761 ttaatatttt accatgcaaa tttaatatct aaatttagaa ttgcatggta aattaacatt
     2821 tttaacaatt actgttgagc tgagaacgga agtttttcaa aaacaagcaa cattctattc
     2881 aaatcagata atgctgatat gaatgcttta atcttctaaa ataaataaca gattttatgg
     2941 ctcaatgaaa tcaaaaatcc caagctgtct tggattcttt tgttctttct tttcgtagcc
     3001 cttgaagccc gccagattca cgtcgattac ctttcgcaaa tacaagttgg gcgtcatgcc
     3061 tttagatgcg caaaattgtg caaatttctc ggctttctga gtcgaaatgc caagtttgat
     3121 ggagcggtat ctttttttct tacgtgccaa aaccgttgta tttgccgcaa aagtaaagaa
     3181 aaattcataa atcctccatt taaacgaggt tgtgaatcgg ggcaacgcat ttaaatgcct
     3241 gtaaataatc ccgattgatg cacccagcgc caacttcaag catgcaatat caacctttgt
     3301 gacacagagt ttaacggagt acacactgag gcgcacagag tagatttatt ctgtcaaaag
     3361 ccgatatcaa tttaatgagc gaataaacac tgtgatacct ctgagaaact ctgtataact
     3421 acggttcatg tggttttttg catgttttaa tgcgttgacc ctgaggtcgt gaacaaggac
     3481 gtgtggcaat cagccgtcag ttggccttgg tgatttcgtg gcaaatttcg ctgcggctct
     3541 gcgccctctg agcgacccac tgaatccgca ttaatcatgc gaaaattgtt cgtgaaaatt
     3601 agtgtaattc gtggatgaaa aagaggattt gctgctgcag ttttccagga gttcccggca
     3661 cacaagaaca ctgaataatg aatactgaat aaaatacaag gaagtttgaa tattgaacga
     3721 tacttcagtt caaatttaac ttcttccttc gatattccat gctcggtgtt cagtgttcaa
     3781 tattcatcag gtgccttggt gttatagtgg caaattatct ctgcgagcca ttattccccg
     3841 ctccgcgcaa taaaacgctg cgcaattttc tcgaaatgct tattctttaa tttcacagca
     3901 attttacggg cgtgttccaa aaaatcgcga ctcgcctctg gcttatactc atacattgaa
     3961 tgaacaacaa gagtcatata atcagccagg tgaaaaccat caggcataat cgggcattca
     4021 atttcttttc ctataaaatt tttaaactga acactgtttt tttcaaatgt tcctgattca
     4081 tccagcgcca gatgcaagta aaacaatagt ttatgtaaac tatccgattc aaacgtaaac
     4141 gtaccatcag caaagtaatt acgaataaat ttatcgaaat atggtgtaaa agcaaatata
     4201 agttctttct gtcctgcaac atacaaagct tcagctattt gaggaatgaa aataacctgt
     4261 ctcgcttcag gaattgaatc aacttctttg agaataaatt gcagtttttt ttcataccac
     4321 gaattgggcg tgcagggcga tgaaaagaat ttgtacataa tctgacaggc atgaaaacgt
     4381 gctacaggat aataatttgg gatgctctca ttggtgattt catcggcatt gtagcgcttg
     4441 aaaagcatat aacccgtctt taaaaattcc ttcaggtttt gtgcatacaa atgtttgtac
     4501 ataatcatac accaggccca gatatcattt tccataaaac tgtcgctggt cggcctgatg
     4561 tttttcagat aatatttttc aaccatatcg ccgtagtaaa caacaatgcc ttctatatca
     4621 accatgttgt tgaaatacag attacgcaag tagttttcgc gagcaatagc tgggatgagt
     4681 gcgtttcgcg cgtcttccga tttaccgatg gcaccggcaa gcaaacggac aagagcaaaa
     4741 tcaccggttt ttgcgatatt ttcaataata accggtgcca ttttatacag gtattcaata
     4801 actggtacat atgctttgtt ttcaagacag ctctgcagca gcattaactc actactgctt
     4861 acacccgaaa acgaattgtc cgacggactt aaaatattat ttcgcccttc aattattttt
     4921 tcatgcgatg aattcacaaa tgaatgccaa tcggtgtatc cacaatattc cgacaatgaa
     4981 tcaagggtgt atttagaggg tgaacttttt gaaaccacaa cacccatcaa acgcgccaat
     5041 gtgaaatccg aaattttgat tccgcattcc gttacaaggt aatccgcaaa ctctttgcat
     5101 tcggccatgt tttccaatcg cctgcccgat tttaaccgga tgcaatcaag taaccttaat
     5161 aaggttattt ctggaatatt tttaatccct acaggcatgt aaaaaaactt ttaagcaaat
     5221 gtacatttta atgcctgaat tattacaatt tggtgtgttt ttttgttaat catgagctgt
     5281 atattcaatt tgttagtttt aatcaaggca atttcgcata catataatac taacaaatac
     5341 gttatcaaaa aacatattgt taataacaat gattaatgta cttttgcatt gttaaataaa
     5401 tatatgaaag ttttttacct gcttgtgttt ttagttttag cttttggtta tttatgtgca
     5461 caggatacca tcattcagct gaaacccgtt gaaatctcaa ccaataaaga tgaaattaca
     5521 cgtctggcac cgcattatga gctcattaat ctggaagaaa ttcagttgca gaccatgtct
     5581 aaagaaatac cggccatact gtcgcaattg ccaggaattc actatgttcc aggtagaggc
     5641 atcggtgata cggaattgcg gattcgcgga tttgatgtca gtaatattca attgctgatc
     5701 aatgatattc ccgaatcgta tgtcgatgaa ggctggacat actggtccaa ttttaccgga
     5761 atcgaagatt ttacacagag cgtttttgtc accaaagggg tcagtaacac gcatccattc
     5821 agcaaagcgg gcggaacaat ttctattcat ctgctcgaag catcagctgc cagatttacg
     5881 cgcttttctt caagctacag ccagttatcg ggcaataaaa tcaatttcac tcacagcagc
     5941 ggattgctca aaaatggctt tgcatgggct gttggtggca cctatcgttt tggttcttcc
     6001 tataaagacg aaacctggtt ccgtggagga aattatttct tcaatgcttc aaaaaaactg
     6061 aataccaaac atacattaca gctgactgtt tttggtgcac cacagcaaca tggtcaggcc
     6121 tccaattatt taccggattc tgttttacag gataaagaac tttcctacaa cccttcgtgg
     6181 ggctatctga atggtgatcc gaaacaaagc aatattaatt tttatcaccg cacattggtg
     6241 caactgaaac atctggccga attcagcaaa aaaaatcatc tgaaatcgat tctcaatttc
     6301 tcaaatggct cgggcggcgc cactgtaaac tttggaaatt ggttgccagg aattgaacag
     6361 attgatttcg atgccgccta ttctgccaat cagaataatt acgattcaac attcggaggc
     6421 aataatgcac tgtattttct gggcgattat gttcataagg aagcaagctt ttcgttgtat
     6481 tccgaattga atcatcactt ttccgattca gcccgcatcg atgcaggcat tctcgcctat
     6541 tcatcgcttt ttattaaaga taacgggcgc atcaacgatt tgcttggcgc cgattttcag
     6601 gtctatccgg gcgaaacctc tgtgcgccat gaaggcgatg acatctggta taaaaataag
     6661 gccagccgac atggagccgg cgtttttgct acgtgggtaa aatcagcaga caatacagat
     6721 ttcatgcttt cgggctcatt tatccgctct acaggcagct ataccgaatt attcaatgtg
     6781 cccgattctg ccatcgagcg gactgatatg aagtggttca acagctacaa tctaaaggga
     6841 tcggtgcagc attatctgac aaaaaatctg gcagtttata ctaccgcagg gtttcagcat
     6901 cgccggcctc cggtcttgtt tatcgggaaa accgttattg ataatgtaat tcctgaaaac
     6961 ttcactactg tcgaagcggg catgcgccga tatttcgaaa gcccgttcca gggccagatt
     7021 catggcagtg tgtattatac ggcgcgccgc aatcagaatt ttgtaaatta ttacaccgac
     7081 ggcctcagtg gtgaaatgtt tactttctca gttgaaggac tcaacagccg ctacatgggc
     7141 gccgaaattg aattcgaagc caggtggtcc gaaaaattca tgggttccgt agctgtatca
     7201 tacaataatg tgatgtggct taacaatatt caaagcaccg taactgacct ctccggcgct
     7261 ccaatcgata ccattgtgct gaacgcgaaa ggaattttcg atggtaatca acctcaatta
     7321 gctgccaata tttcgggact gtacaccatc agatcgcatt accagatttt tgcagaagca
     7381 ctttataacg gtctcaatta cagctggttc aatgttctaa attatactgg aagcaacgaa
     7441 gtaaaacaaa gctggcagat tccggattat gtaatttttg atctgggcat cgcatacaaa
     7501 accaagcttc gaaacaaagt cgatatgcgc ctttcggcta ctattcacaa tgtgttcgac
     7561 aatcatttta ttgccgaagg tatcgatgga gccaaccacg acaagaattc atcctatgtt
     7621 ctgtacggcc atggacgctg tctgacagct ggtgttgtgc ttgagtttta attttgaatc
     7681 tctctctgta agtaaaaagt atataaagtg gaatgttcct aaaggcgcat taagtaatca
     7741 tttaaaggca cctttctact tgaaaacttt ctactttatg actaatattc actggcgcgg
     7801 atttgtaatc gtgacagtgc tatcacctgt ttttttgcaa tccttcaatc cgtgacagta
     7861 ctctcttcag tctttttatt ataagtatta tactataagc ttcctgttat atatctaatc
     7921 aaac
//
LOCUS       DAOPKE010000120         7723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_10088_length_7723_cov_13.095331, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000120 DAOPKE010000000
VERSION     DAOPKE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10088_length_7723_cov_13.095331"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKY63_11530"
     CDS             <1..193
                     /locus_tag="PKY63_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01291.1"
                     /translation="VKVKVKVKVKVKVEVEVELLSAETARPRLRLSGLQQFSVSSETR
                     LGSKHATCALAPLCTMPYA"
     gene            complement(198..2411)
                     /locus_tag="PKY63_11535"
     CDS             complement(198..2411)
                     /locus_tag="PKY63_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01292.1"
                     /translation="MAKSKVESWFLNAVQDNKKIQVHCRLKPVITDNYVSLGQLTALR
                     FLEWVCEHPGGVIALPTGKTPEFFIKWTQFYLANWSRELDKGILSEIGFKSGVNPDMK
                     SLEFFQLDEFFPIDPTHERSFSHFINTFYIKGFGLDRRKAHLINTSDMPDRGNMNLCE
                     LFSDGEIDLSLRTRQVKSEKELHKKRAIQYIDQMCAEYEESIRNAGGIGFFLGGIGPD
                     GHIAFNIRGSRHDSVTRLDHINYETQAAAASDLGGIENVRRKAVVTMGLQTITANPDC
                     TAVIIAAGASKSKVVADAILNEPSPDFPATCLHKLPNARMFITRSAASGLIEEKFPAS
                     AISVSAIKDKIQRGLDLPKGKVIMHTAPHHDDIELAYFPAIHHLVRSDKNENHFVYCT
                     SGFTAVTNIYLASVLEATAAFLSSSDIQDKASEIKLFDASSKNYDITGYLNGIAQQQK
                     EIQLLHIACRMVRNVASHLNSRSWEKIKKFISSLAGIIQSLEPGSREPEEIHLAKGWV
                     REFEAELAWAHFGLPDSYTHHMRLKFYSADIFPEYPNYNEDVLPIFNLMKKIKPDVIT
                     LAIDPEGSGPDTHYKSLMAISAAIDEYVKQTGRSDIRIWGYRNVWSHFKLGEVNTIIP
                     VSLNSLAVLHNMFNTCFLSQRSASFPSYAFDGSFSELAQNIWVHQFNELRTILGDKFF
                     YESPSRMLNRAYGAIYMSDMSYQEFTEYLVPLRRLLESKSLLEKKRNEIRDRHKA"
     gene            complement(2557..3729)
                     /locus_tag="PKY63_11540"
     CDS             complement(2557..3729)
                     /locus_tag="PKY63_11540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPF01293.1"
                     /translation="MSESATLAMTKKSREMKATGIDVINLSIGEPDFFVPDFIKAAAK
                     KAVDDNFSFYPPVAGYPQLRQAISNKLKRDNGLEYTPEQIVVSTGAKQAICNVFLACL
                     DPGDEVIVPAPYWVSYTELIKLAGGVPVYIPTSIEDDFKVTPEQVEKMITPKTKAFIF
                     CAPNNPSGMLYTLDELKALAAMFGKHPDILIISDEIYELINFDGKTYSIGIFPEVKDR
                     TVTINGLSKAFAMPGWRIGYSAAPLDISKAIEKVQGQVTSAANAIAQIAGVTALQVDP
                     AQCEDLQKMKKAFLSRRDYVVKRLGDIPGFKPNVPMGAFYVFPDVQQLYGKSDGTSVI
                     NNDSDLCIYILNKAHVALVPGSAFGDPNCIRFSYATSMENLTKALDWIEKAIKELK"
     gene            4660..6585
                     /locus_tag="PKY63_11545"
     CDS             4660..6585
                     /locus_tag="PKY63_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPF01294.1"
                     /translation="MFSINELLVEFPGRVLFDRIGFVVNARDRIGLVGRNGAGKSTLL
                     KILAGLDSGYSGHLGFPKDKTVGYLVQERSFTSAKTVMEEAETAFEAVHKLMAESERL
                     TKELIERTDYESDSYLALSEKLHEITERLHLNGGSNYQEDIEKVLKGLGFAREEMFRP
                     MAEFSGGWQMRVELAKLLLKRPDLLLLDEPTNHLDIESIRWLEGFLQNYPGAIMLVSH
                     DRVLLDNVTTRTIEIQQGKLYDYPANYSDYVELRQERIEQQSNELKNQQREIAQIEDF
                     IERFRYKATKAKQVQSRIKRLEKFEDITIDDTDHSRMAFRFAPAKESGKIVVEAQQVT
                     KSYGEKLVIKPTDFIIARNERIAFVGQNGQGKSTLVKMIMSTIDFGGELKLGHNVQIG
                     YFAQNQTEMLDRNKTIFETLEDVADNETRPKLRNILGSFLFSGEDVDKKVMVLSGGEK
                     TRLALAKMLLLPINFLVLDEPTNHLDMAAKDVLKNALLHFNGTLIVVSHDRDFLSGLT
                     TRIFEFRDHQIRQLDYDIHELMEIRDQEQAAALKIQRNSSGEKEISASKQQYLDSKEK
                     DRQKRKIERQISEAETSISEIESAMLIIENQLNSPGQISNSQDYSHLATQYTNLKKQL
                     EQTMAEWESLNSQLENI"
     gene            6931..7623
                     /locus_tag="PKY63_11550"
     CDS             6931..7623
                     /locus_tag="PKY63_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HPF01295.1"
                     /translation="MKITLFTLGALLCIIGVNAQSFTMNNGSVTTCNGYFYDSGGSAG
                     EYYSNSDLIYTVKNPQGQGELSVEFIEFALGDGDWLSVYEGQVDESSLIETYDKNNLM
                     KTKLTTMKGSFVFVFHSDAAGSGPGWKAVFACPGNPQVEPVLDNGSIAILIYSVRGLK
                     SEDDAILLDKHLKTLGLVIKTSFNIEKENAWITADQELTYKIKDALLDTKNLLGYEIA
                     VDYQTSIQAKTK"
BASE COUNT         2080 a         1542 c         1945 g         2156 t
ORIGIN      
        1 ggttaaggtt aaggttaagg ttaaggttaa ggttaaggtt gaggttgagg ttgagctgct
       61 ttcagcagaa acagcaaggc caaggttgag attaagcggc ttgcagcagt tttctgtcag
      121 ctcggagacg agactgggtt caaagcatgc gacatgtgcg cttgctccgc tttgcacaat
      181 gccttacgcc taatgcctta cgccttatgc ctatctcgta tttcgtttcg ttttttttcc
      241 agcaacgatt tcgattcaag caatctgcga agcggaacaa gatattcagt gaattcctga
      301 taactcatgt cactcatgta aatggctcca taagcgcggt tgagcatgcg gctcggactt
      361 tcgtaaaaga atttatcgcc gagaatggtg cgcagttcat tgaattggtg cacccagata
      421 ttttgtgcaa gttcgctgaa agatccatcg aatgcatagc tgggaaacga ggcggagcgc
      481 tggctgagga agcatgtgtt gaacatgttg tgcagtaccg ccagtgagtt gagcgaaacc
      541 ggaatgatgg tgttcacttc gcccagcttg aaatgcgacc acacgttgcg gtagccccag
      601 atgcggatat cgctgcgtcc ggtttgtttt acatactcgt caatggcagc agaaatggcc
      661 atcagcgatt tgtaatgtgt gtcggggccg ctgccttcgg ggtcgatggc gagtgtaatc
      721 acatccggtt ttattttttt catcagattg aaaatcggca gcacatcttc gttgtagtta
      781 ggatactccg gaaaaatatc ggctgagtaa aatttcaatc gcatgtgatg cgtgtaggaa
      841 tcgggcaggc cgaaatgcgc ccaggcaagt tcggcctcga attcgcgcac ccagcctttg
      901 gcgagatgta tttcttccgg ttcacggctg ccgggttcga gcgattgaat gattccggcg
      961 agcgaactga tgaatttttt gattttctcc caactgcgcg aattcaggtg cgaagccacg
     1021 ttgcgaacca tgcgacaggc aatatgcagt agctggattt ctttctgttg ttgcgcaata
     1081 ccattcagat agccagtgat atcgtagttt ttggatgagg catcgaataa tttaatttct
     1141 gatgctttgt cctgaatgtc gctactactg aggaatgcag ccgttgcttc gagaaccgat
     1201 gccagataaa tattggttac ggcggtgaag ccactggtgc aatacacgaa atgattttcg
     1261 tttttgtcgc tgcgcacgag atgatgaatg gccgggaaat aggccagttc aatgtcatcg
     1321 tgatgcggtg cggtgtgcat gatcactttt ccttttggaa ggtcaagccc gcgctggatt
     1381 ttgtctttga tggcgcttac cgaaattgcg gatgcaggga atttttcttc aatcagtccc
     1441 gatgcggcgc tgcgtgtgat gaacatgcgt gcattgggaa gtttatgcag acatgttgca
     1501 ggaaaatccg gcgagggttc attgagaatg gcgtcggcaa cgacttttga ttttgaagca
     1561 cctgcggcaa taatcacagc cgtgcaatcg ggattggccg ttattgtttg caaacccatg
     1621 gtaacaactg ctttgcgacg cacgttttcg attccaccca gatcggaagc agcggcagcc
     1681 tgcgtttcgt agttgatgtg atcgaggcgt gtcactgaat cgtggcggct gccgcggata
     1741 ttgaatgcaa tatgtccgtc aggaccaatg ccacccagaa aaaaaccgat gccgcctgcg
     1801 ttgcggatag attcttcgta ttcggcacac atctgatcga tgtattgaat ggctcttttt
     1861 ttatgcagtt ctttttccga tttcacttgt ctggtacgca gactcagatc aatctctcca
     1921 tctgaaaaaa gttcgcacag attcatgttg ccacggtcag gcatgtcgga ggtgttgatc
     1981 agatgcgctt tgcggcggtc gaggccaaag cctttgatat aaaaagtatt gatgaaatgc
     2041 gagaaagagc gttcatgcgt cgggtcgatg ggaaaaaatt catcgagctg aaaaaattcc
     2101 agcgatttca tgtctgggtt aacccctgat ttgaagccga tttcggaaag aatgccttta
     2161 tcaagttcac gactccaatt ggcaagataa aactgagtcc acttgataaa aaattccgga
     2221 gtctttccgg taggcaaagc aattacaccg cccggatgtt cgcataccca ctcaagaaat
     2281 cgcagcgcag tgagttgtcc gagtgaaaca taattgtcgg taatgacagg tttcaggcga
     2341 caatgcacct gaattttctt gttatcctga acagcattca gaaaccagga ttcaacttta
     2401 cttttggcca taggacacca tttttataag tcaatccccc cggaaataac agtgcgagtg
     2461 tgagagcgag aatgagagaa agaattttga aaatgctgct gtcaattctt cgactccgct
     2521 cagaatgaca gcagcattat ttgtgcagca ttgattctat ttcaattctt tgatggcttt
     2581 ttcaatccag tcaagcgctt tggtcaggtt ttccatgctg gtggcgtagc tgaagcggat
     2641 gcagtttgga tcaccgaaag cggagcccgg aaccagtgcg acatgagctt tgttgagaat
     2701 gtagatgcaa agatcgctgt cgttgttgat gactgaggtt ccatccgatt ttccatagag
     2761 ttgttgtacg tcggggaata catagaaagc gcccatggga acattgggtt tgaatcccgg
     2821 aatatcgccc agacgtttta ccacgtaatc gcgacgagag aggaaagctt ttttcatttt
     2881 ttgcagatcc tcgcactgag cgggatcgac ctgaagtgcc gtaactccgg caatctgtgc
     2941 gatggcattt gctgcggaag ttacctgccc ctgtactttt tcgatggctt ttgagatatc
     3001 gagtggtgca gcgctgtaac caatacgcca gcccggcatg gcaaatgctt ttgaaagtcc
     3061 gttaatagtc accgtgcggt ctttcacttc tgggaaaatg ccgatgctgt atgtttttcc
     3121 atcgaaattg atgagttcat atatttcatc agagatgatg agaatgtcgg gatgtttacc
     3181 gaacatggca gccagcgcct tgagttcatc aagggtgtag agcatgcccg aaggattgtt
     3241 gggggcgcag aaaatgaacg cttttgtttt tggcgtaatc attttttcga cctgctccgg
     3301 agtgacttta aaatcatctt cgatgcttgt cggaatatag acaggcacgc caccggcgag
     3361 tttgataagt tcggtatagc tcacccagta cggagcggga acgattactt cgtcgcccgg
     3421 gtcgaggcag gcaagaaata cattacagat ggcttgtttt gcaccggtag acacaacaat
     3481 ttgttcaggg gtgtattcaa gtccgttatc gcgtttcagt ttgtttgaaa tggcctgacg
     3541 cagctgcgga taaccggcta cgggcgggta gaatgagaaa ttgtcatcga ctgctttttt
     3601 ggcagcagct ttgataaaat caggaacaaa aaagtctggt tcaccgatgg aaagattaat
     3661 tacatcgatt ccggttgctt tcatttcgcg gctttttttg gtcatggcca gcgtagccga
     3721 ttcgctcatt ttggttatac gctcagagag aaatgtattt ttcacgacta ttattttttg
     3781 atgttgcagc aaagatagta attttagtat tcagtattca gtggtcagtt ttgtctttcg
     3841 ttacacgtta caaacgttac acaggtgaca gggtaacaag gtgacagggt aacaaaagtt
     3901 gcatttcagc tgtcggctct gagaaggagc ttaggacgtg gcatgcggca tgcggtgcac
     3961 aacacgcaac gcctgatgca caacgcacaa caactaatgt cttccgcctt acgcctaaca
     4021 ccttctgcct caccgcaggt gcttaaccct caacctcctt gaatcatccc gccgaagcgg
     4081 gacaagttcc caacgaacaa ggaattacga aggaagaagt gaagtttgaa gtaagtataa
     4141 taaagtaccg ttcgatatta ttacttcctt gttccatgtt cagagttctc gatcttgtcc
     4201 ttaacctcga tcttgttctt gtccttgtcc tcaaccttct tgaatcatcc cgccgaagcg
     4261 ggacaagttc ccaacgaaca aggaattacg aaggaagaag tgaagtttga agtaagtata
     4321 ataaagtacc gttcgatatt attacttcct tgttccatgt tcagagttct cgatcttgtc
     4381 cttaacctcg atcttgtcct tgtccttgac ctcaaccttc ttgaatcatg aataacgaac
     4441 aaggattttt gaattaagaa gtgaagtgtg aagtaaaata caatgatgta tcgttcgata
     4501 ttcttacttc cttgttcctt gttcagagtt cttgacctta acatcgactt tctcagcctc
     4561 gtctcagagc tgacggctga caactgatag ctgaaggctt tttgtccccg acctcgttgt
     4621 gagtctttcc ggttcattgt ttatttataa ttttgcgtca tgttttcaat taatgaattg
     4681 ctggtagagt ttccggggcg cgttttgttc gatcgcatcg ggtttgtggt gaatgcgcgc
     4741 gaccgcatag ggcttgtagg gcgcaatgga gcagggaagt caacgttgct gaaaattctg
     4801 gctggccttg attcaggtta cagcgggcat cttggatttc cgaaagataa gacagttggc
     4861 tatctggttc aggaacgcag tttcacatcg gcaaagaccg taatggaaga agctgaaacc
     4921 gctttcgaag ccgttcataa gctgatggca gaatcggagc gattgacaaa agaacttatc
     4981 gagcgcactg attatgaatc ggactcgtat ctggctttat cggaaaaact gcatgaaatc
     5041 actgaaagac tgcatctgaa cggcggcagc aattatcagg aagacattga gaaagtatta
     5101 aagggcctgg gattcgcacg tgaagagatg ttcaggccaa tggccgaatt cagcggcggc
     5161 tggcagatgc gtgttgaatt ggcaaagcta ctgttgaagc gccccgatct attgttgctt
     5221 gatgagccca ccaaccatct ggatattgag agcatacgat ggcttgaagg atttcttcag
     5281 aattatccgg gtgcaattat gctggtatcg cacgaccgcg tattgctcga caatgtgact
     5341 acgcgcacca ttgagataca acagggaaaa ttgtatgatt atccggcgaa ttattctgat
     5401 tatgtagaac tgcgccagga gcggattgaa cagcagagca atgagctgaa aaatcagcag
     5461 cgcgaaattg cgcagattga agattttatt gaacgtttca gatataaggc caccaaagca
     5521 aagcaggtcc aatcgagaat aaaacgtctg gagaaatttg aagatatcac catcgatgat
     5581 accgatcatt cgcgcatggc tttccgcttt gcacctgcta aggagagcgg caaaattgtg
     5641 gtcgaagcac agcaggtaac taagtcttat ggcgaaaaac tagtcatcaa acccaccgat
     5701 ttcatcattg cacgtaacga gcgtattgcg tttgttggac agaacgggca ggggaaatcg
     5761 acgctggtga aaatgatcat gagtacgatt gatttcggtg gcgagctgaa actgggacac
     5821 aatgtgcaga taggatattt tgcgcagaac cagaccgaga tgcttgaccg caacaagaca
     5881 atattcgaga cactcgaaga tgttgccgat aatgagaccc gccctaaact gcggaatatt
     5941 ctcggtagct ttttgttcag cggtgaagat gtggataaaa aagtgatggt gttgtcgggc
     6001 ggcgaaaaaa cgcggttggc gcttgcaaaa atgttgttgt tgcccatcaa tttccttgtg
     6061 ttggatgagc cgaccaatca tttggatatg gctgccaaag acgttttgaa gaatgctctg
     6121 ctgcatttca atgggacgtt gattgtggtt tcgcacgacc gtgatttttt gtcgggattg
     6181 accacgcgta tttttgaatt ccgcgaccat cagatccggc agctggatta tgacattcat
     6241 gagttgatgg aaatccgtga tcaggagcag gctgcggcgc taaagattca acgaaattca
     6301 tccggagaaa aggaaatcag tgcatcgaag cagcagtatc tggatagcaa ggaaaaagat
     6361 cgtcaaaagc gaaaaataga aagacaaatt tccgaagccg aaacgagcat cagcgaaatc
     6421 gagtcagcta tgttaatcat tgaaaatcag ttaaatagcc ctgggcagat ttctaacagc
     6481 caggattaca gtcatttagc tacccaatac accaatctta agaagcagtt ggagcaaacc
     6541 atggcggagt gggaaagttt aaacagccag ttggaaaata tttaagtggt tgattaacat
     6601 tgttatatcg gttaaaacaa tcttttctat gggcgaaaac cgtcaggtta tcaaccaatg
     6661 ttgataattc ggctaaatat tttcaggaca acattaaact ttttacaatt ttaattgtct
     6721 aaggggtaaa ttttccaaaa ctgaatgatt tatttacctg gcgaaagtga gaaagaagga
     6781 tttgatacaa ggtttttatt gtctttttgt cttttgtaca ggtaaagagt ttggttgcgg
     6841 gaaaaatgga aagccatatc tgaatagtct cgggggggat tatcggtgtg gttttccgcg
     6901 ttttaacagg ggaacaaata aaaaatacat atgaaaatta cactttttac gttgggagct
     6961 ttgttatgca ttataggggt gaatgcgcag tcgttcacga tgaataatgg ttcggtcact
     7021 acctgtaatg gatattttta tgacagtggc ggtagtgccg gggagtatta ctccaattcg
     7081 gatttgattt atacggtaaa aaatcctcaa gggcagggcg agctctctgt tgagtttatt
     7141 gagtttgcct taggcgatgg tgattggctt tcggtgtatg aaggtcaggt agatgaatca
     7201 tcgctgattg aaacatacga taagaataat ttgatgaaaa caaagctgac aacaatgaaa
     7261 ggaagcttcg tttttgtttt tcattcggat gctgctggaa gtggtccggg atggaaggca
     7321 gtgtttgcgt gcccgggtaa tcctcaggtg gagccggtgc tggataatgg ttctattgct
     7381 attctgattt attctgtcag agggctaaaa tcggaggatg atgctatcct acttgataag
     7441 cacctgaaaa cgcttggtct ggtaataaaa acaagcttca atattgaaaa ggaaaatgca
     7501 tggattacgg cagaccaaga gctgacctat aaaatcaagg atgcgttgct cgatacaaaa
     7561 aacctgttgg gatatgaaat tgcggtggat tatcagacca gcattcaggc gaaaacaaag
     7621 taattggttt tatcaatcga aaaattagag tcatgacaaa taaagggtta aaattagttc
     7681 agaacatttt caaagtaagc ctcatattca ttttttcttt aat
//
LOCUS       DAOPKE010000121         7580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_10357_length_7580_cov_11.889967, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000121 DAOPKE010000000
VERSION     DAOPKE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10357_length_7580_cov_11.889967"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1483)
                     /locus_tag="PKY63_11555"
     CDS             complement(86..1483)
                     /locus_tag="PKY63_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPF01296.1"
                     /translation="MITFEIQTAAYLFASILFILSLGGLSSQESAKRGVFFGIAGMFI
                     AVLATVFGQQSEGHIYILGAILIASVIGILVARKVEMTAMPQLVAILHSFVGMAAVLV
                     GFGSFLDPDTAKLQGSEFTIHLVEIFIGVFIGAITFSGSVVAWGKLQGTIRSKALVFK
                     GRHLLNATMVAASVALGVLFVMQHETNGMMWLLVMTGIALIIGFMLVMGIGGADMPVV
                     VSMLNSYSGWAAAAAGFMLGNDLLIVTGALVGSSGAILSIIMCRAMNRSFISVILGGF
                     GQTSHSAAAMEGEVTSVNHNEAARILSEASSVVIVPGYGMAVAKAQYPVHELSDLLTE
                     RGAKVRFGIHPVAGRLPGHMNVLLAEARVPYDIVLEMDEINPDLPSTDVVMIIGANDI
                     VNPGAQDDAGSPIYGMPVIEAWKAKRVIVLKRSMATGYSGVENALFYKENTDMLYGDA
                     RESVEKLNTAIRELH"
     gene            complement(1488..3020)
                     /locus_tag="PKY63_11560"
     CDS             complement(1488..3020)
                     /locus_tag="PKY63_11560"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPF01297.1"
                     /translation="MIIGILKERQAGEMRVAITPKTVQRLVKKGFIIQVESEAGILAG
                     FNDNDYTQAGAQIIAGADQLLSVADIIMKVQPPVEAEIEKMKAGAVTLSYLKPAQNED
                     LMKVLASHNINAIAMDAIPRISRAQKMDVLSSMANIAGYRAVIEAAGYYGRFLNGQIT
                     AAGKVEPAKVLVIGAGVAGLAALGAANSMGAIVRAFDTRKEVAEQIQSMGAQFLTVNI
                     DEDGATSTGYSKQMSQAFIDAEMELFRQQASEVDIIITTAQIPGKPAPKLILKEHVAL
                     MKPGSVIVDLAASTGGNCELTKDGEVYTTENGVIIAGKTDPLPGQASQLYSSNLCNLL
                     DDMGDAASFKIDMKDDIIKRAMVTYNGSVNWPPEPLPVSATPAQKITAAAKPPTEPQS
                     SARRKTISSAIWLAVIGGLLYLLGQYAPPVFMGHFTVFVLAVFVGWQVIWNVTHALHT
                     PLMSVTNAISGIIIIGGLLQMSNEINSTVSIIAFVAILIASINIVGGFLVTHRMLNMF
                     KK"
     gene            3342..7577
                     /gene="glgP"
                     /locus_tag="PKY63_11565"
     CDS             3342..7577
                     /gene="glgP"
                     /locus_tag="PKY63_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPF01298.1"
                     /translation="MNMQQMEKPDFLFEVSWEVCNKVGGINTVIATKALRISERIETG
                     YIMIGPDIHSGQLPAEVFTEDNSIHPQWVSKARMEGFHFRIGRWHVPGNPIAILLDFS
                     GLYSKKDIVFKELWEKYTLDSLYGGWDYVEPTLFGYEAGRLIEHFYNYNVTSEDRIAA
                     HFHEWMTGAGILYLKDRVPQAGRVFTTHATVMGRSVAGMNLPLYSKMEEFNPEGMARE
                     LNVLSKFSLEKLSAQHADVFTTVSEITGKECTHFLSKKPDLITPNGFEDTMVPHEEEF
                     NNRRAASRKLLKSLVRSVIGKDVADNTLYVINSGRYEFLNKGIDLFIEALADLKIHHK
                     TDRDVVAIIAVPANNAGVRQDVKQCMMEDEITQAPGNLTHYLFNEDNDAIMQAIHNNG
                     LQSDVESNVNVVFIPSYLDGNDGLLNFNYYDFLIGCDLSVFPSYYEPWGYTPLEAVAF
                     HIPTVTTTLAGFGVWMQNAGKVNGGVKVIERDDFNADFVVSEISRYIQEWALCSREEF
                     QLHRESALHASQEALWENLSKHYFDAYHRAIQHSVNRREHFSKKTLPQEHVLEKTGVA
                     EPNWRKIYVKPELPERLSKLMRLSKNIWWTWSHSAIRLFESINPQLWERSGYNPLAML
                     ELMSLKEINELATNPEFLLRLDMVFREFEEYMDLPLDEERPSVAYFCMEYGFHASLKL
                     YSGGLGVLAGDYLKQASDSGIRMLAVGLLFRQGYFNQTFSAGGEQISEYIPQKFSNLP
                     MLPVRDVKGDWVKISVSFPGRLVVAKVWEVHVGRVRLFLLDTDIDDNNEQDRQITHKL
                     YGGDWENRLKQEILLGIGGVKMLEYLGLTFDIYHCNEGHAAFSILERLKDYVQNNVMS
                     FQQALEIVRAQTLFTTHTPVPAGHDAFSEDMMRMYFAHFPEKLNLSWKEFIALGKQNP
                     DNADEKYSMSILAMSGSQEVNGVSRIHGRVTREMFTNLYQGFFSEELYVNYVTNGVHL
                     PTWCSQEMQNLAKKYGFDPLKSDQADPANWEFVHQIPDDELWNMRKSLKGKLLDMLRN
                     KLARDMTHRQESPRHIINVLEHFDDNALYIGFARRFATYKRAHLLFKNLDQLRQLVNN
                     KTRPIRFVYAGKAHPADKAGQDYIKYIIEISRMPEFAGKIIFVENYDMEIARLLVAGV
                     DVWLNTPTRPLEASGTSGIKACLNGVLNLSVLDGWWAEGYVPKGGWALKEVRTYVDQS
                     LQDELDSETLYNLLEEEILPLYFKREQNDIPAEWLAFIRKNFTAIAPNFTMKRMLDEY
                     YSKFYVPQLQRSRDLRKDNYAAARALSSWKRRVMRNWDQIIATEIMVHDSTVSPLLLG
                     QDFVASVTLNLGELRAEDLALDVIFGQKEKDHVQNIIFKTEMKVKSFGNGQATYEVVI
                     PNPRSGVFDYAIRLRPSNPLLPHLQDFNLVKWL"
BASE COUNT         2070 a         1634 c         1771 g         2105 t
ORIGIN      
        1 aaaaaaaccg aagggcctga ttcatcaatc aagcccttcg aaagtattga aatattattg
       61 aatgtgataa cggatcaact caagttcaat gcagctcgcg gatcgctgta ttcagttttt
      121 ccacactctc ccgtgcatca ccgtagagca tatcggtatt ttccttgtaa aaaagcgcgt
      181 tttcgactcc cgaatagccg gtggccattg atcgtttgag aacaattacg cgtttggctt
      241 tccatgcttc tataacgggc attccgtaaa tcggacttcc tgcatcgtcc tgtgcgcccg
      301 gatttacaat gtcgttggcg ccgataatca tcactacatc agtcgatggc agatcgggat
      361 tgatttcatc catttcgagc acaatgtcat aaggtactct ggcttcggcc agcaacacat
      421 tcatatgtcc cggcagccgg cctgccaccg ggtgaatacc gaaacgcacc tttgcaccgc
      481 gctcagtgag caaatccgat aattcatgca ctggatactg tgctttggct actgccatgc
      541 catagccagg aacaatcaca actgaagatg cttcgctcag aatgcgtgca gcttcgttat
      601 gattcaccga ggtcacttct ccttccatgg cggctgcgga atgcgaggtt tgcccaaatc
      661 cgcccagaat caccgatata aatgaacggt tcatggcgcg gcacataatt attgaaagaa
      721 tggcacccga actgcccacc aacgcaccgg tgacaatcag caaatcgtta cccagcatga
      781 agcccgctgc tgctgcagcc cagcccgaat aggagttgag cattgatacc accaccggca
      841 tgtcggcgcc accaattccc ataaccagca tgaatccgat tatgagggca atgccagtca
      901 taaccagcaa ccacatcatt ccattggttt cgtgctgcat gacaaataaa acaccgagtg
      961 caaccgaagc tgctaccatc gtggcattca acagatgacg tcctttaaaa accagcgctt
     1021 tgctacgtat ggttccctgc agttttcccc aggcgacaac cgatcctgaa aatgtgatgg
     1081 ctccaatgaa aacgccaata aaaatttcaa ccagatgaat ggtgaattcc gatccttgca
     1141 gctttgccgt atccggatca agaaaagatc cgaagccaac cagtacggca gccattccta
     1201 caaaactgtg taaaatggca accagttggg gcatggccgt catttcgact tttcgggcta
     1261 ccagaattcc gataaccgaa gcaatcagaa tggctccaag aatgtagata tgtccttcgg
     1321 actgttgtcc gaaaacggtc gccaatacag cgataaacat acctgcaatg ccaaagaaca
     1381 caccgcgttt ggccgattcc tgcgaagaca atccgcccag actgagaata aacagaattg
     1441 acgcaaataa ataagctgct gtttgtattt cgaaagtaat catgatttta ttttttaaac
     1501 atgttcaaca tccggtgagt aaccagaaat ccgccaacaa tattgatgct tgcaatgaga
     1561 attgctacaa aagcgatgat tgaaacagtg ctgtttattt cattgctcat ctgcagtaat
     1621 ccgccaataa tgatgatgcc gctgatggca ttggtaaccg acatcaacgg ggtatgcagg
     1681 gcgtgagtta cattccatat tacctgccag ccgacaaaaa cggccagtac aaacacagtg
     1741 aaatgtccca taaacactgg cggtgcatat tgtccgagca gatacagcag tccaccgatc
     1801 actgccagcc aaatggctga tgaaatggtt ttgcggcggg cactgctttg tggctctgtt
     1861 ggcggttttg ctgctgctgt tattttttga gcaggcgttg ctgaaactgg aagtggctct
     1921 ggcggccagt ttacagaacc attatatgtt accatagccc gcttgatgat atcgtctttc
     1981 atatcgattt taaaactggc ggcatcgccc atatcatcga gcagattgca caaattgctg
     2041 ctgtagagct gacttgcctg accgggcagg ggatcggtct tgccggcaat gatgacaccg
     2101 ttttcggttg tgtacacttc gccatctttt gtcaactcac agtttccgcc ggtcgatgct
     2161 gccagatcaa caatgaccga tccgggtttc atcagagcca catgttcttt aagaatcagt
     2221 ttgggagccg gtttgccggg aatctgtgca gtagtgataa taatatcgac ttcggacgcc
     2281 tgttgacgaa agagttccat ttcagcatca ataaaggcct gactcatttg cttgctgtaa
     2341 cctgtcgatg tggcaccgtc ttcatcaata ttgacggtta gaaactgagc tcccatcgat
     2401 tgaatctgtt cggcaacttc tttgcgggta tcgaaagcgc gcacaatggc acccattgaa
     2461 ttggctgctc caagtgccgc aaggccagcc acaccggcac caatcaccag cactttagct
     2521 ggttccactt tgccggcagc ggtaatctgt ccgttcaaaa agcgaccata atatccggct
     2581 gcttcaatca cagcgcgata accggcaata ttcgccatcg acgaaagtac gtccattttc
     2641 tgagcacgcg aaatgcgcgg aatggcatcc atggcaatgg cgttgatgtt gtgactggcc
     2701 aatactttca tcaaatcttc attctgagcg ggttttagat aacttagtgt tactgcaccg
     2761 gctttcattt tttcaatttc agcttcaacc ggaggctgaa ctttcatgat gatatcggcc
     2821 actgaaagta attgatcggc tcctgcaata atctgggcac cggcctgtgt ataatcattg
     2881 tcattgaaac ctgccagaat gccggcttcg gattcaacct gaatgatgaa tccttttttc
     2941 acaagtcgct gtacggtttt gggcgtaata gcaacgcgca tttcgccggc ctgtctttct
     3001 tttaaaattc ctattatcat agcaaaaaaa tgatgattga gtttgtcaaa attatacatt
     3061 tttgatgcgc acaaatctga aaatttttat tcaaaacctg atatgaatca ttttatttca
     3121 ttttgaaata ccaatagaag ctatgctcgt tgatatgaac ccgtctgtct aaatcattca
     3181 ttaaggcgcg ggcaattccc atttcgttta tcaatacttt tcaaccaaca cgaatagtta
     3241 tccacattta acgcgttgtt ttatgacagt gtattatttt tgtctgggtg gggcaaaatt
     3301 gtttcacatg atttgaattt ttcgtaataa aacagcggca tatgaacatg caacagatgg
     3361 aaaaaccgga ctttcttttt gaagtaagct gggaagtatg taacaaagtt ggtggcatca
     3421 acacagtgat agctacaaaa gcgcttcgta tcagcgagcg tattgaaacc ggctacatca
     3481 tgataggccc tgacattcat tcgggtcagc ttcctgccga ggttttcacc gaagacaaca
     3541 gcatacaccc gcaatgggta agcaaagcgc gcatggaagg attccatttt cgtattggcc
     3601 gctggcatgt gcccggaaat cccattgcaa ttctgctcga cttttccgga ttgtattcaa
     3661 aaaaagatat tgtctttaaa gaactgtggg aaaaatatac acttgattcg ttatatggtg
     3721 gctgggatta tgttgagcca acattgttcg gttacgaagc cggacgattg attgagcatt
     3781 tctacaatta caatgtaaca tccgaagacc gcattgcagc tcattttcat gaatggatga
     3841 cgggtgccgg gattctttat ctcaaggatc gtgttccgca ggcaggacgt gtttttacaa
     3901 ctcatgctac tgtgatgggt cgctccgttg caggtatgaa tcttccactt tatagtaaaa
     3961 tggaagaatt caatccggag ggaatggcac gtgaactgaa tgtgttatcg aaattcagtc
     4021 tcgaaaaact cagtgctcaa catgccgatg tttttacgac tgtttcggaa ataaccggaa
     4081 aagaatgcac gcattttttg tcgaagaaac cagatttgat tacccccaat ggcttcgaag
     4141 acaccatggt tccgcatgaa gaagaattca acaatcgccg tgcggcttca cgaaaattgt
     4201 tgaaaagtct tgtcaggagt gtgatcggaa aagatgtggc cgacaacact ttatatgtca
     4261 tcaattctgg tcgctatgaa tttctcaata aaggaattga tttgtttatt gaagcgctgg
     4321 cagatctgaa aatccatcat aaaactgatc gtgatgtggt tgccattatt gcggtaccgg
     4381 ccaataatgc aggtgtgcgt caggatgtga agcaatgcat gatggaagat gaaatcactc
     4441 aggcacccgg aaatctcact cattatttat tcaacgaaga caacgatgct attatgcagg
     4501 ccattcacaa taatggctta cagagcgatg ttgagagcaa tgtaaatgtg gttttcattc
     4561 ctagttatct tgatggaaat gacggtttgc taaatttcaa ctattacgat tttttgattg
     4621 gttgcgatct ttcagtattc ccttcgtatt acgagccatg gggttatact ccgttggaag
     4681 ctgtggcttt ccatattccg acagttacaa caacactggc cggttttggc gtctggatgc
     4741 agaatgcagg caaagtaaac ggaggtgtga aagtgattga acgcgatgat tttaatgccg
     4801 attttgtggt tagtgagatt tcgcgttata ttcaggaatg ggctttatgt tcgcgcgagg
     4861 aatttcagct tcatcgcgag agtgctttgc atgcatcgca ggaagcttta tgggaaaatc
     4921 tgtcgaagca ttatttcgat gcttatcacc gggctattca gcattcggtt aatcgacgcg
     4981 aacatttcag caaaaagacc ctgcctcagg aacatgtgtt ggaaaaaaca ggtgtagctg
     5041 aaccaaactg gcgaaaaata tatgtgaaac cggaattgcc tgagcggttg agtaagctta
     5101 tgcgtttgag caaaaatatt tggtggacct ggagccattc tgctatccgg cttttcgaat
     5161 ccatcaatcc gcagttgtgg gaacgcagtg gctataatcc actggccatg cttgaactca
     5221 tgtcgttgaa agaaatcaat gaattggcca ccaatcccga atttttgctg cggcttgaca
     5281 tggttttccg tgaatttgag gaatatatgg atctgccact cgacgaggaa cgtccttctg
     5341 tggcgtattt ttgtatggaa tatggttttc atgcatcgct gaaattatac tccggtggct
     5401 tgggagtact tgcgggtgat tatctgaagc aagcatcaga cagcggaata cgaatgttgg
     5461 cggtgggtct tcttttccgt cagggttatt tcaatcagac attttcggcg ggaggggaac
     5521 agatatcgga gtatattccg caaaaattca gcaatctgcc gatgcttccg gtacgtgatg
     5581 taaaaggtga ttgggtgaaa atttcggtga gctttccggg tcgattggtg gtcgcaaaag
     5641 tttgggaagt ccatgtgggg cgggttcggc tgtttttact tgataccgac attgatgaca
     5701 acaatgaaca ggatcgtcag ataacccata aactttatgg tggagattgg gaaaatcgtt
     5761 tgaagcagga aattctgctg ggcattggtg gtgtgaaaat gctggaatat ctgggactga
     5821 cttttgatat ttatcattgc aatgaaggac atgcggcttt ttcaatattg gagcggctga
     5881 aggattatgt acagaacaat gttatgtcgt ttcagcaggc acttgaaatt gtccgcgctc
     5941 agacattgtt tacgactcat actcctgttc cggctggtca tgatgctttc agcgaagata
     6001 tgatgcgaat gtattttgcc cactttcccg aaaagctgaa tttatcatgg aaagaattca
     6061 tcgcacttgg aaaacaaaat cctgataatg ctgatgaaaa atacagcatg agtattcttg
     6121 ccatgagcgg gtcgcaggaa gtgaatggtg taagtcgtat tcatggccgt gttactcgtg
     6181 aaatgtttac taatctgtat cagggatttt tctccgaaga gttgtatgtc aattatgtaa
     6241 caaatggagt tcatcttcca acttggtgca gtcaggaaat gcagaatctt gcaaagaaat
     6301 atggattcga tccgcttaaa tcggatcagg ccgatccggc caactgggaa ttcgttcatc
     6361 agattccgga tgatgaattg tggaatatgc ggaaatcgct gaaaggcaaa ttgctcgata
     6421 tgctgcgcaa taaactggcc cgcgatatga cgcatcggca ggaatcgccg cgacatatta
     6481 tcaatgtgct ggaacatttc gatgataatg ctttgtatat tggctttgcg cgccgttttg
     6541 cgacatacaa acgtgctcat ttgctgttta aaaatcttga tcaattgcga caacttgtca
     6601 acaataaaac acggccgatt cgttttgttt atgccggaaa ggcgcatcca gccgataaag
     6661 cggggcagga ttatattaaa tacattatcg aaatttcgcg catgccggag tttgccggaa
     6721 aaattatttt cgtcgaaaac tacgatatgg aaattgcacg acttctggtt gccggtgtcg
     6781 atgtatggct caatactccg acccggccgc tcgaagccag tggtaccagc ggtatcaagg
     6841 cctgtctgaa cggagtgttg aatctgagtg tacttgatgg ctggtgggcc gagggttatg
     6901 ttccgaaagg cggatgggct ctcaaggaag tccgcacata cgtcgatcag tctctgcagg
     6961 atgaacttga ttcagaaaca ttatataatc tgcttgaaga agaaatactt cctttatatt
     7021 tcaaacgcga acaaaacgat attccggccg aatggcttgc ttttatccgc aaaaatttca
     7081 cagccattgc cccgaatttc accatgaagc gtatgctgga tgaatattat tcgaaattct
     7141 atgttccgca attgcaacga agcagggatt tgagaaaaga taattatgca gcggcccgtg
     7201 ccttgtcttc atggaaacga cgcgtgatgc gaaactggga tcagattatt gctactgaaa
     7261 ttatggtgca cgacagtact gtgagtccgc tgttgctggg acaggatttt gttgcttcgg
     7321 tgaccttgaa tcttggtgaa ttgcgggccg aagacctggc tttggatgtg atttttggac
     7381 agaaagaaaa agaccatgtt caaaatataa ttttcaaaac cgaaatgaaa gtaaagagtt
     7441 ttggcaatgg acaggcaacg tacgaagttg ttattccaaa tccgcgttca ggcgtatttg
     7501 attatgctat ccgtctgcgt ccatcgaatc cactgttgcc acatctgcag gattttaatc
     7561 tggtgaagtg gctgtaaaaa
//
LOCUS       DAOPKE010000122         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_10384_length_7565_cov_13.952064, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000122 DAOPKE010000000
VERSION     DAOPKE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10384_length_7565_cov_13.952064"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1184
                     /locus_tag="PKY63_11570"
     CDS             42..1184
                     /locus_tag="PKY63_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPF01299.1"
                     /translation="MAWLKKWYVWLPFLILIFALVYFFSDILIYILLAFIISLMGNPL
                     VRFLDSLRFRKLRIPHSISALISLVTILFVLVSLVLLIIPVIINQAEYLSQVDINGIL
                     TSMQEPINDLEESLRKYGFLAADGSIESSLNSYMNEFLKSVNVQDFAGSLLGMVGSLS
                     IGVFSVIFLSFFFMRDDQLFYKGLKAFTPRDSHDEIARILFYSKKMLSRYFIGLFTEL
                     FCVITLISIGMYIIGLENAIFIGVVAGVFNIIPYLGPIIGGAIGCLVGLTTLSPELFA
                     DQSGMLLIEMLVVFISTNLIDEFVLQPLIFSRSVKAHPIEIFIIILMAGTIAGPVGMI
                     IAIPSYTLLRIIAIEFFSHWDFVQNITGNLNKELQKSRGEEPPANE"
     gene            1177..2157
                     /locus_tag="PKY63_11575"
     CDS             1177..2157
                     /locus_tag="PKY63_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF01300.1"
                     /translation="MNESLTILKSLSLGRLFNLFLTIISETLSRLFRKPVLLNQPWAA
                     SIEPSAVCQLSCPECPVGMKEISRKNNFLSNDHFAVILNGISKKTFWLNLYFQGEPLL
                     NKNIAEKIKMARSRKMFVVLSTNALALDKNMAMQLCNAGLSKIIISLDGADEKTYLKY
                     RSGGDFQKVLDAISYLHDARKKNKSPLIEVQMLVFSYNENQKETLLEMALNLGADKVV
                     FKSPQFYNTENASKNLPSEKKYQRYKKNPEGKIQLKTKYKKFCKRLWNTIVINSDGDV
                     VACCYDKFSSYKMGNAIEKPVVGIWSSTAFMQFRSDFLKGKRKDICRNCE"
     gene            complement(2290..4218)
                     /locus_tag="PKY63_11580"
     CDS             complement(2290..4218)
                     /locus_tag="PKY63_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPF01301.1"
                     /translation="MKTILIIVSCLLLSASVIAQTPMLQGKVIDKETREPIPFAHVYW
                     CDGKTGALTDLEGDFLIADSAGCDSLIVSYPGYFKHIIYVKSDIENVVIELSADEIYL
                     SDVIVHSYDEISVTCDYESVAKMSDYKSVSHRKPKLARGDKAEGSADMPVSWTEVAGG
                     AVPSSGISAGQLTAGEVNDFTKWKLWEDINTTDLASYNKKWTLYPENRFVVQVTNKKK
                     LPVHNATAVLMDETGNVVWSARTDNTGKAELWAGMFDGKADKKYSINVIYENKTYPLK
                     KVDSFQNGINFIELPVNCSEEKNVDIAFMIDATGSMGDEIAYLQSELSDVVERVKDSL
                     PGNSIRLAIVFYRDMGDAYVVIQKDFTSNIPEAIKYLRSNGAGGGGDYPEAVDSALHI
                     SVNNLAWSDNAVTRILFPVLDAPPHETQAIITSIQNSVSRAAAKGIRITPLACSGVNK
                     STEYLLRSMALATNGTYVFLTDHSGIGNPHIAPTTDEYKVEKLNDALVRIIVSFSESP
                     DCDKPITENMNVPDTAVMNMPAEKDSTQINDSIVSMGDDSLKIDTVLPLQPEISWRYY
                     PNPTAGPVTVEIVNLPEAKEGFLYLTDLSGKLLARYEVKESNVIEIDISMYPTGTYYL
                     TFFYGDESKLCAPVVLMH"
     gene            complement(4290..>4871)
                     /locus_tag="PKY63_11585"
     CDS             complement(4290..>4871)
                     /locus_tag="PKY63_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPF01302.1"
                     /translation="ILRIAKPILRIAMKGFKFKISDQTKPYFLTMTLVGWVNLFGAEK
                     YRNIVLDALKFCQACKGLRIYAWVIMPSHIHMIADSKNGTLSKTIGEFKRHTSKHIFK
                     TLQEEDEFDSSEFISFFSDISTLHSRNNDYQVWKQDNHPVELFSKEFIYNKIDYIHNN
                     PVKSGLTSRVEDYPFSSARNYAGLTGILDVEIA"
     assembly_gap    4872..4971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4972..6629)
                     /gene="ade"
                     /locus_tag="PKY63_11590"
     CDS             complement(<4972..6629)
                     /gene="ade"
                     /locus_tag="PKY63_11590"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPF01303.1"
                     /translation="MKISGQIVDVRTRRIFAGEVVVENGKISAINELDTAPEQFIMPG
                     FIDAHVHIESSMLAPSRFAELAVKQGTVATVSDPHEIANVLGVEGVKFMIENGKTVPF
                     HFFFGAPSCVPATPFETAGFSINSTETAELMSNPDIWYLGEMMNFPGVVFDDSEVHAK
                     IKAALDAGKPVDGHAPLLSGDHLKKYAAAGISTDHECTNIEEAEEKIKLGMKILIREG
                     SAARDFDMLFPLIDKYPSDVMLCTDDCHPDELMRGHINVKVKMAIERGVDLFNVLQAA
                     CINPIEHYKLPVGTLKAGDNADFIVVGNWREMDVLQVYIKGEKVFDNGSVLIESKPFI
                     SINNFARTDVMPSDFAVEAKGNNIKVIVAKDGELLTESEVVSAKIENGCIVPDVENDI
                     LKMTVVNRYNNSKPAVAFIKGIGIKKGALACSIAHDSHNLIAVGTCDEAIALALNKII
                     ESKGGLAAVDSVSSEHLALPLAGLMSDRKGEEVAALYHALNQKASEMGSPLRAPFMTL
                     AFMALLVIPKLKLSDKGLFDGTTFAFTPLCEVYTSHSETNTSHS"
BASE COUNT         2212 a         1748 c         1360 g         2145 t
ORIGIN      
        1 tacgatttca tagataatcg ctacctttac aaaaaaaatt tatggcatgg cttaagaaat
       61 ggtatgtctg gcttcccttt ttgattctga tttttgcact ggtctatttc ttttcagata
      121 ttctgattta tatccttctg gcttttatca tttctctgat gggaaatccc ctggtccggt
      181 ttctcgatag tctcaggttt cgcaaactca gaattcccca ctcaatcagc gctttgattt
      241 ctctggttac aattctgttt gttctggtat ctcttgtact tctgattatt cctgtaatta
      301 tcaatcaggc ggagtatttg tcgcaagttg atatcaacgg aattctcaca agcatgcagg
      361 aacccatcaa cgacctggag gaaagccttc gtaaatacgg attccttgca gcagatggca
      421 gcattgaatc atcgctgaat tcatatatga acgaatttct gaagtctgtc aatgtacagg
      481 atttcgccgg ttcactgctt ggaatggttg gctcactaag catcggcgtg ttttctgtca
      541 ttttcctgtc attctttttc atgcgcgatg accagctttt ttataaagga cttaaagcct
      601 tcactccacg cgattcgcat gatgaaatag cccgcatact tttctacagc aagaaaatgc
      661 tttcgcgtta tttcattggt ctgttcaccg aacttttctg tgtgataact cttatttcca
      721 tcggcatgta catcattgga ctcgaaaatg ccattttcat aggtgttgtt gctggagtat
      781 tcaatatcat cccttacctt ggtcctatca tcgggggcgc catcggctgc ctggtcggtc
      841 tcacaacact ttcgcccgaa ctctttgccg accaatcagg catgctcctt attgaaatgc
      901 tcgtcgtatt catttctacc aacctgattg atgaatttgt tctgcagcca ctcattttct
      961 cgcgaagcgt aaaggcacac cccatcgaaa tttttataat catcctgatg gcaggaacca
     1021 tagcagggcc agtcgggatg atcattgcca tcccttccta tacactgctg cgcattattg
     1081 ccatcgaatt tttcagccat tgggattttg tccagaacat caccggaaat ctcaataaag
     1141 aactgcaaaa atccagaggt gaagaaccgc cggcaaatga atgaatcttt gacaatatta
     1201 aaatccctgt cgcttggcag attgttcaat ctttttctga ccatcatttc agaaacgctc
     1261 agccgccttt tccgaaaacc ggtattgctc aatcaaccat gggctgcatc aattgaacca
     1321 tcggccgtat gccagctttc atgccctgaa tgtccggttg gtatgaaaga aatttccaga
     1381 aaaaacaatt tcctttcaaa cgatcatttc gctgtcatct taaacggtat cagcaaaaaa
     1441 acattctggc tcaatctcta ttttcaggga gaacctttgc tcaataaaaa cattgctgaa
     1501 aaaataaaaa tggccagatc gcgcaagatg tttgttgttt tgagcacaaa tgctctggca
     1561 cttgacaaaa acatggccat gcaattatgt aatgccggtt tatcaaaaat catcatttca
     1621 cttgatggcg ccgatgaaaa aacatatctg aaataccgct ccggaggcga ctttcaaaaa
     1681 gtactcgatg ccatttccta tttacacgat gcaagaaaaa aaaataaatc tccgctgatt
     1741 gaagtgcaaa tgcttgtttt ttcctacaac gaaaatcaaa aagaaaccct tctggaaatg
     1801 gctctgaatc ttggcgccga caaagtcgtt tttaaatctc ctcaattcta caatactgaa
     1861 aatgcatcaa aaaatcttcc ttccgaaaaa aaataccaac gctacaaaaa aaatccggaa
     1921 ggaaaaattc aactgaaaac aaaatacaaa aaattctgca aacggctttg gaacaccatt
     1981 gtcattaatt ccgacggtga tgtggttgca tgttgttacg ataaattttc ttcctacaaa
     2041 atgggcaatg caatagaaaa accagtcgtc ggaatatgga gtagcacagc attcatgcag
     2101 ttccgcagcg atttccttaa aggaaaaaga aaagacattt gtagaaactg cgaataaata
     2161 aaccctcgaa gacagcatag ctcttggctt gcatagggtt tattccactc tcacttcgct
     2221 aacatcggat ttggtttcca aatagcaagt tattctattt tgcaaacata cgagggttta
     2281 agcttcctct taatgcatca gcaccaccgg cgcacacagc ttgctttcat cgccatagaa
     2341 aaaggtaaga taataggtcc cggttggata catgctgata tcaatctcaa taacattact
     2401 ttccttcact tcgtatcgcg ccaacagctt acccgagaga tcggtcagat acaaaaaccc
     2461 ttccttggct tcaggcagat tgacaatttc caccgttaca ggacccgctg tgggattggg
     2521 ataatatcgc caactgattt ccggctgcaa tggaagaacg gtgtcgattt tcaacgaatc
     2581 atcacccatt gatacaattg aatcattgat ttgagtgctg tccttctcag ctggcatgtt
     2641 cattaccgcg gtatcgggta cattcatgtt ttccgttatt ggtttatcac aatcaggcga
     2701 ttcgctgaat gaaacaatga tacgcaccaa tgcatcgttg agtttttcaa ctttgtattc
     2761 atcggtagtt ggcgctatat gcgggttccc aataccactg tgatcggtca gaaaaacata
     2821 tgtaccatta gtagcaagcg ccatagaacg aagcagatat tcggtacttt tattcactcc
     2881 gctgcatgca agcggcgtga ttcgtattcc tttggctgca gcacgcgaaa cagaattctg
     2941 aatgcttgtg atgattgcct gcgtttcgtg tggtggtgca tcaagcacag gaaaaagaat
     3001 acgtgttaca gcattgtcac tccaggcaag attatttaca ctgatatgta atgccgaatc
     3061 aacggcctcg ggataatcac caccaccacc ggctccgttg gatcgcaaat actttatggc
     3121 ctccggaatg ttgcttgtaa aatctttctg aatgacaaca tatgcatcgc ccatgtcgcg
     3181 gtaaaataca atggcgagac gaatactgtt cccgggcagt gagtccttca cccgctccac
     3241 cacatcgctg agctccgact gcagataagc aatttcatcc cccatcgaac ctgtggcatc
     3301 aatcatgaaa gcaatgtcca catttttttc ttcggaacaa ttcaccggaa gttcaataaa
     3361 attaatcccg ttttgaaacg aatccacttt ctttaaagga tatgttttat tctcataaat
     3421 gacgttgatt gaatattttt tatctgcttt cccatcaaac atacctgccc atagctcggc
     3481 ttttccggta ttatcagtcc tggcagacca cacaacattg ccagtttcat ccatcaagac
     3541 cgcagttgca ttatgcacag gaagtttttt cttattggtt acctgcacca caaaacggtt
     3601 ttcgggatat aatgtccatt tcttattata acttgccaaa tctgttgtat tgatatcttc
     3661 ccacaatttc catttcgtaa aatcattcac ttcacccgca gtaagctgcc ccgcagaaat
     3721 ccctgatgat ggtacagccc cacctgcaac ctctgtccat gaaaccggca tatcagcaga
     3781 accttcggcc ttatcgcctc ttgcaagctt tggcttccta tgactcacag atttataatc
     3841 agacatttta gccacacttt cgtaatcaca tgttactgat atttcatcat aagaatgcac
     3901 aatgacatcc gacaagtaaa tttcatcagc cgataactca attactacat tttcaatatc
     3961 tgatttcaca tatattatat gtttgaaata gcccggatat gatactatca acgaatcgca
     4021 tcccgcagaa tcagcaatca gaaaatctcc ttcaagatca gtcaaagcac ctgttttacc
     4081 atcgcaccaa tatacatggg caaaggggat gggctcacgg gtttctttat caataacttt
     4141 cccctgcagc atgggagttt gggcaatcac gcttgccgac agcagtaagc atgaaacaat
     4201 gatgagtata gttttcatat tttttcgggt ttaatgggta taggatgcac cagtcgcgaa
     4261 agttcacaaa aatgaataag cacctgcaac tatgctattt ctacatccaa aatcccggtt
     4321 aaacccgcat aatttcttgc acttgaaaaa ggataatctt cgacacggct tgtcaggccg
     4381 cttttcacgg gattattatg tatgtaatct attttattat aaatgaattc ttttgaaaac
     4441 aattcaaccg gatgattatc ctgtttccat acctgataat cattgttgcg ggaatgtagt
     4501 gttgagatat cactgaaaaa gctgataaac tcacttgaat caaattcatc ctcttcctgt
     4561 agtgtcttga aaatatgctt gcttgtatgt cgcttgaact caccgatggt tttactcagt
     4621 gtcccattct tactatcagc aatcatatga atatgactgg gcataattac ccatgcataa
     4681 atccgcaacc ccttgcaagc ttgacaaaac ttcaaagcat cgagcacaat attcctgtac
     4741 ttttcagcac caaacaaatt cacccagcca accaaagtca ttgtcaaaaa atacggcttc
     4801 gtctgatcag aaatcttgaa tttaaagccc ttcatcgcta tgcgaagtat tggtttcgct
     4861 atgcgaagta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgctatgc
     4981 gaagtattgg tttcgctatg cgaagtatat acttcgcata gcggagtaaa tgcaaaagtc
     5041 gtcccgtcga aaagtccctt gtcactcagt ttcagctttg gaatcacgag aagtgccata
     5101 aacgccagcg tcataaaagg cgcgcgcagc ggcgatccca tctccgatgc tttttgattc
     5161 aacgcatgat acaatgcagc cacctcttcg cctttgcggt ccgacatgag cccggctaat
     5221 ggcaaagcca gatgttccga cgaaacagaa tccaccgcag ccaatccacc cttgctttca
     5281 attattttat tcaaagccaa tgcaatggct tcgtcgcatg taccaacagc aatcagattg
     5341 tgactatcat gtgcaatcga acaagccagc gctccctttt ttatacctat tcctttgata
     5401 aacgcgacag ctggtttgct attattgtaa cgattgacga cagtcatttt caaaatatca
     5461 ttttcaacat cgggaacaat acaaccattt tcaattttgg cactcacaac ttcgctctcg
     5521 gtgagcaatt cgccatcttt ggcaacaatg actttaatat tattcccttt tgcttcaaca
     5581 gcaaaatccg aaggcatcac atctgtgcga gcgaaattgt tgattgagat aaatggtttt
     5641 gattcaatca acacagatcc attatcaaaa actttttcac ctttgatgta aacctgtaac
     5701 acatccatct cacgccagtt accaacaaca atgaaatcgg cattatcacc tgctttcagc
     5761 gtcccgaccg gcaacttgta atgctctatc ggattaatgc aggccgcctg caaaacattg
     5821 aataaatcaa caccccgctc aatcgccatt tttactttca cattgatgtg accacgcatc
     5881 aactcatccg gatgacaatc atcggtgcac aacatcacat cggatggata cttatctatc
     5941 agaggaaaaa gcatatcaaa atcgcgtgca gcactgcctt cgcgaatcag tattttcatc
     6001 ccgagtttga ttttttcttc cgcttcttca atattggtgc attcatgatc ggtgctaata
     6061 ccagcagctg catatttttt taaatgatcg cccgaaagca aaggagcatg tccgtccacc
     6121 ggctttcctg catccagtgc ggccttgatc tttgcatgca cttcggaatc atcaaaaaca
     6181 acacccggga aattcatcat ttcgcccagg taccaaatgt ccggattgct cattaattca
     6241 gcggtttcag ttgagttgat tgaaaatccg gcggtctcaa aaggggttgc aggcacacac
     6301 gaaggtgctc cgaaaaagaa atgaaacgga acagttttcc cattctcaat cataaatttc
     6361 accccctcca caccaagcac attggctatt tcatgcggat ctgaaacagt tgctacggtg
     6421 ccttgtttca ctgccagctc tgcaaaacgc gaaggagcca gcatggagct ttcgatatgc
     6481 acatgcgcat cgataaagcc aggcattata aattgttcgg gagcagtatc caactcattt
     6541 atagcagaaa ttttaccatt ttcaacaaca acttcgcctg caaaaatgcg acgagtacga
     6601 acatcaacga tctgaccgga aattttcata acaacatatt tgttattaca aatatagtgt
     6661 ttatttaaaa tcggcactat actttcaact cattataaca cacaaaagaa tgagataaac
     6721 tcattggaac attacatcgt caaaatcata tttaataaat atcattcagg caaaatcttc
     6781 aaatttactg catgaaaaac gcaacgcctt caaaagaaaa gaaatcgctc atattcgata
     6841 tgcgcaaagc gaaacatcat cgcaggcgca aattaccaaa tcaactcaag acaaaagaga
     6901 ccaactaata ttctcacata tgcggagcgg aatttaatcc tccgcctcca gcggagtaaa
     6961 ctccaaaggc ggatcatgga cgaagtccca catttccaca tccgttcaag ataaaaaata
     7021 aaagtaatac caaaatgaaa tatataacag tctgatttca atggatgact ccggaggagt
     7081 cgcatgtctg tagaaatgat ataaataaac tacgactcca gcggagtcga atgtatttat
     7141 accaaatgga aatatataat agtcaattgt agcaatttca ttgaacgttt tgttggctga
     7201 tgcctgatgt ttacctgaca aaacaggcag gcatcgcata tctgtagaaa aaccaatacc
     7261 gaccacaaga tccgccggct ggcggatcgc atattaacaa gcattgtcag aaaacgaagc
     7321 tccttatatg cgactcctcc ggagtcgtgg ttttgtgtga tcctggtcta cagatatgtg
     7381 actcctccgg agtctcccaa aattggattg taccgatttg taaaaagctt tggtattaaa
     7441 ccacatctga cagggagctg tttacatggg atcagccagc catatgctga gctgacgaag
     7501 catggcggat cagccatcaa tctgcactta gacacattgg aataaaatat atttcacgat
     7561 ggtat
//
LOCUS       DAOPKE010000123         7277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_10894_length_7277_cov_13.558156, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000123 DAOPKE010000000
VERSION     DAOPKE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_7277_cov_13.558156"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PKY63_11595"
     CDS             complement(<1..1664)
                     /locus_tag="PKY63_11595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPF01304.1"
                     /translation="MRLILILAFIGFCFTALAQVSSNAHPPMTNPILLTSNFAEYRKN
                     HFHSGIDVSVRNRSNMNVFSIWDGYVSRVRYNSASYGRAVYITHPNGYSSVYGHLDSF
                     APFIDSLVLEYQYANKVFETDIQIPAGKVPVKKGQILGIAGNSGYSFGAHLHFEIRDA
                     ASDDPVNPLKFIPVDDLMEPQFRQLCIYRLQPGGFMPATATYHKTIKKGNIFTTTTEI
                     IVPDTFFIGLDVQDYQSYSYYRLLPQQIEISIDDSTVWKINFDRFAFDQSGACRGVFD
                     HTAGLKYNKQIVTTYTGNHLRQRFFKQYSNDGLFVLPDEKPHKLTIKATDTEFNTSVF
                     STTIKKGKTLPQTTGKYIVRTRYFHDFSTEHLTIKCDTGTFYSDLALDEPLLTEYNSG
                     QDMYWKIGFNDIPLIKPIHVLFDPIDNNDKILLAKMSGKNIDKVWKPALNQDNKMTAD
                     IDRCGIFKLITDTLAPVISKTNVPSTGKITWQKFITLVVTDNSGDIDEFNAYINGEWT
                     LMTYDLKSDTFTIPLNTAQRAVLKTLRVEFIDLAGNKTEREYRFVK"
     gene            complement(1720..2163)
                     /locus_tag="PKY63_11600"
     CDS             complement(1720..2163)
                     /locus_tag="PKY63_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01305.1"
                     /translation="MKKLLFPLMILLIAMGSCTTSSQLTGNEDEYELLKKGYNRISVK
                     DYKGGIQIFTKVAEMNPDNAEAIAYRGLCKYHLKDFEGAIRDYNKALEIQPGYAEVYD
                     LRGIAKGELGDKEGACADWKKAFELGLKGAFDLIKEFCWDEQIEN"
     assembly_gap    2400..2499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2795..3829
                     /gene="porQ"
                     /locus_tag="PKY63_11605"
     CDS             2795..3829
                     /gene="porQ"
                     /locus_tag="PKY63_11605"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPF01306.1"
                     /translation="MKHFILLISLFMPLGLMAQNGVNKVFSFLELSNSSRLTALGSNM
                     ASIHDNDVSLVLSNPSYICDSMHSTLALNYTNYFSDINYGFVTYSHTFEKAGSFAASV
                     QYLNYGNFQETDIYGNALGNFNAYDMALTMGWGRKLHDNFSIGANAKFIYSDLYKAVS
                     SGVAVDVAGSYFNDDKYLSATLVVRNIGRQLNTYFQGVSEPLPFDVQLGVSKRLLHMP
                     LRFSLLAHHLHRWDIRYDNPADAEYDPLTGELIEERKLSAFGDKLMRHFVIGAELVPG
                     KSFSFRIGYNYQRRKELTVDSRLSTVGLSWGFGLRIKKFYFSYARSAYHLAGSPNVIT
                     LSTKIDDFLK"
     gene            complement(3914..4696)
                     /locus_tag="PKY63_11610"
     CDS             complement(3914..4696)
                     /locus_tag="PKY63_11610"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPF01307.1"
                     /translation="MLYIKIRFSTFLQTSEHPEAGQGTHIRLHRHEADIHKILENELY
                     YYKTLSTENKRIFIRRVIKFNMSTVFYPQAGMKLGPRHVILISATFVQITFGLEKSLL
                     GYLNNIKIFPAHYFDPRTKRQYKGEVDVNGMICLSWEDFEIGLRDSNDGRNVGLHELA
                     HAFAIEIIEKDTNYHHLLMKLKPVFLRAKFEINNPYHRHGLLREYGYTNMHEYFAVST
                     ELFFEKPDYLANSGSPLYSELCMVFNQDPYKQYLAYETQKMA"
     gene            complement(4754..5533)
                     /gene="kdsB"
                     /locus_tag="PKY63_11615"
     CDS             complement(4754..5533)
                     /gene="kdsB"
                     /locus_tag="PKY63_11615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPF01308.1"
                     /translation="MKFIGIIPARYASLRFPGKPLAIIAGKPMVQWVYENASKADSLS
                     QVFVATDDNRIFQKVEFFGGKAIMTASNHPSGTDRCFEAAILSGADANDDDTVIINIQ
                     GDEPFIDPVIIDQLAAAFKDPQVNIATLVRPFDSDEALFSENCMKVVVNTANNALYFS
                     RSIIPFIRNPRRDKFLFEQFPFRQHIGVYAYRIKTLSQLIKLQPSRLEQAESLEQLRW
                     LENAYSIRIIETRYRGHSVDVPSDIEILKEHFPDIFRNSLI"
     gene            complement(5535..6149)
                     /locus_tag="PKY63_11620"
     CDS             complement(5535..6149)
                     /locus_tag="PKY63_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPF01309.1"
                     /translation="MHIAIAGNIGSGKTTLTGLLAKHYKYAPQYEDVDTNPYLTSFYE
                     DMKRWSFNLQIYFLNSRFRQIVELHRQAKDVIQDRTIYEDACIFAPNLHDMGLMSSRD
                     FATYQSIFELMVSFIKAPDLLIYLRADVPTLVSQIQKRGRDYEASIRLDYLKSLNERY
                     ESWINEYKDGKLLIIDVEKVKFHEKKEDLGVIIEKINAELFGLF"
     gene            complement(6218..7018)
                     /locus_tag="PKY63_11625"
     CDS             complement(6218..7018)
                     /locus_tag="PKY63_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01310.1"
                     /translation="MKHFYFSILFLHMCMLTYSQSPHPVREYKLSEQAAAFSVDPMGS
                     VYVVFENGSLLKFDTTGTPVAIPGRKSKSSFWQIDASDPYKIIVFNRDLQIADIYSSD
                     FAKINNIDFSMLETGDISLMCSSYDNAFWTLSALNMDLVRLSEQLTITSKTNAGLLLD
                     FKNFTPTILQESGTNILLAQKGGSAYIFDRFGNLRMHFNEKADYWSLDGDVLYYVLND
                     TLHAYQTQLHKEAIIETGCEKINGIAVSSQWMAILNEKKITLFRKSNN"
     gene            7189..7272
                     /locus_tag="PKY63_11630"
     tRNA            7189..7272
                     /locus_tag="PKY63_11630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7223..7225,aa:Leu,seq:caa)
BASE COUNT         1934 a         1383 c         1577 g         2283 t
ORIGIN      
        1 tatttcacaa accgatactc tcgctctgtt ttgttaccag ccagatcaat aaattctaca
       61 cggagtgttt tcaagacagc tctttgtgcg gtattcagtg gtatcgtaaa cgtgtccgat
      121 ttcaaatcat aagtcatcaa tgtccattct ccattgatat aggcgttgaa ttcatcaata
      181 tcgccactgt tatcagtaac cacaagtgta atgaattttt gccatgtgat tttccctgtg
      241 gagggaacgt tggttttcga aatcactgga gccaatgtgt ctgtgatgag tttgaatatg
      301 ccacatcggt caatgtcggc agtcatcttg ttgtcttgat tcagagctgg tttccagact
      361 ttgtcaatgt ttttccccga catttttgcc agcaggattt tatcgttatt gtcaatcgga
      421 tcaaacaaaa catgaatggg ttttatcaga ggaatgtcgt tgaatccgat tttccagtac
      481 atgtcctggc ctgaattgta ttccgtcagg aggggttcat ccagtgcgag atcgctataa
      541 aaagttcccg tatcgcattt gatggtgaga tgttcggttg aaaaatcatg aaaataccgg
      601 gttcgtacaa tatatttgcc tgttgtttgt ggcagtgttt ttcctttttt gatggttgtg
      661 ctgaataccg acgtgttgaa ttcagtatcg gtggctttga tggtgagttt gtgaggtttt
      721 tcatcgggca gcacaaataa tccatcattt gaatattgtt taaaaaatcg ttggcgtaaa
      781 tggttgcctg tataagttgt aacgatttgt ttattgtatt ttagcccggc tgtatgatca
      841 aaaacacctc tgcatgcgcc cgattgatca aaagcaaagc ggtcgaagtt gattttccat
      901 acagtggagt catcgatgga gatttctatt tgttgtggca atagccggta gtaagagtat
      961 gattgataat cctgcacatc gagaccaata aaaaatgtat cgggaactat aatttccgtt
     1021 gttgttgtaa aaatatttcc ttttttgatg gttttatggt aggtggctgt tgcaggcatg
     1081 aaaccgccgg gttgcaatcg atatatgcat agttgccgga actggggttc cattaaatca
     1141 tcaacaggaa tgaattttag tgggtttacg gggtcatcac ttgctgcatc acggatttcg
     1201 aaatgcaggt gggctccgaa cgaatagccg ctgttgcctg caattccgag tatctgtcct
     1261 ttttttaccg ggactttgcc tgccggaatc tgaatatcgg tttcaaaaac tttgttggca
     1321 tactgatatt ccagtacaag ggaatcgatg aatggggcaa aggaatcaag gtgtccatat
     1381 actgatgaat aaccattggg gtgggtgata taaacagctc ttccatacga ggctgaattg
     1441 tatcgcacgc gtgacacata gccgtcccag atggaaaaaa cattcatgtt gctgcggttg
     1501 cgtacactca cgtctatgcc tgagtgaaaa tggtttttgc ggtattctgc aaaattgctg
     1561 gtaagcagta tcggattggt catgggcgga tgtgcattgc tgctcacctg tgccagtgct
     1621 gtaaaacaaa acccaataaa agcaagaatt agtattaatc gcataaaaaa agctcccttt
     1681 gggagctaaa attaataaat tcggttgaaa taaccaggat taattttcga tttgttcgtc
     1741 ccagcaaaat tctttgatca ggtcaaatgc gcctttaagg cccagttcaa aggccttctt
     1801 ccaatcggca caggcgcctt ccttgtcacc gagttcgcct ttggcaatgc cgcggaggtc
     1861 atatacttcg gcatatcctg gttgaatttc caatgctttg ttgtaatcgc ggatagctcc
     1921 ttcgaaatct ttcagatggt atttgcaaag tccgcgatag gcaatagctt ctgcattatc
     1981 gggattcatt tcggcaactt ttgtgaaaat ctgaatgccc cctttatagt ctttgacaga
     2041 tatgcggttg tatccttttt tcagtaattc gtactcatcc tcgtttccgg tgagctgact
     2101 gctggttgta cagcttccca tggcaatgag caaaatcatc agtggaaaga gtaacttttt
     2161 catttttctc agattggtaa tgtccccgtt tgtacgatga taacaatatt cggtttcaaa
     2221 attacgaaaa tttctgaata tgaaatattt aaaatgttat taatatcaaa atcggacaat
     2281 tataaggctg cgaattccaa tttggtcggt gaaatattga acaagatata gatcgaccac
     2341 catgaatcat gaacatggaa caaggaattt tgaaggaaga agtaatctcc ttaaatcatn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aacatggaac aaggaatttt
     2521 gaaggaagaa gtaatctcct taaatcatga acatagaacc tgtctgcaat tttaactgca
     2581 acaggcagtc aggcagtcaa ggaattatga agtaagttta aagtatcctt caacgttctc
     2641 acatccttgt tcgttatttc ttgatcaata ttccttcata attctcacat cctcttgatc
     2701 cgtaaggatg cacggattgt tccttgttca atgttcagac ggtctcaata aaataatttc
     2761 tatctttact cgttgttaga cacaaaatca ttccgtgaaa catttcattc tgttgatatc
     2821 attgtttatg ccactgggtc tcatggctca aaatggcgtt aataaagtgt tttcgtttct
     2881 cgaattatca aatagctccc gcctcactgc cctgggctct aatatggcca gtattcacga
     2941 taatgatgtc agtcttgttt tgtcaaatcc ttcgtatatc tgtgattcaa tgcattcaac
     3001 gctcgcattg aattatacca attatttttc cgatatcaat tatggttttg taacttattc
     3061 tcatactttc gaaaaagcag gcagctttgc tgcttcagtt caatatctga attatggaaa
     3121 ttttcaggaa acagatattt atggaaatgc gctggggaat ttcaatgcgt atgatatggc
     3181 tctgactatg ggttggggtc gcaaactgca tgataatttt tcaatcggag ccaatgctaa
     3241 attcatttat tccgatttgt acaaagcggt ttcttcgggt gtggcagtcg atgttgccgg
     3301 tagttatttc aatgatgata aatatctgag cgcaaccctg gttgtacgaa atattggccg
     3361 acagctgaat acatattttc agggggtttc cgaaccattg ccattcgatg tgcagctggg
     3421 tgtttcaaaa cgtcttttgc atatgccgct tcgcttttca ttgttggctc atcatcttca
     3481 tcgctgggat atcagatatg ataatccggc cgatgccgag tatgatccat tgaccggtga
     3541 attgattgaa gaaagaaagt tgagcgcttt tggcgataaa ctgatgcggc attttgttat
     3601 cggtgctgaa cttgttcctg gaaaatcttt ttcattccgc atcggataca attaccagcg
     3661 aagaaaagag cttacggtcg attcacggtt gtcaaccgtg gggttgagct ggggtttcgg
     3721 actccgcatc aaaaagtttt atttttccta tgcgcgttct gcttatcatc tggcgggttc
     3781 tcccaatgtg attaccctgt ccacaaaaat tgatgatttt ctgaaatagg acgttacaag
     3841 cgttatgcgt tacaggcgta acggaaaagc catcagctct gaaaagaagc tgagaagttt
     3901 catgctgcat gccttatgcc attttttgtg tctcatacgc cagatattgt ttatacggat
     3961 cctgattgaa taccatgcaa agttcgctat agagcggtga tccggagttt gcaagataat
     4021 caggtttttc gaaaaacaat tcagttgaaa cggcaaaata ttcgtgcatg tttgtatagc
     4081 catattcacg cagcaatcca tgccggtgat atgggttgtt gatttcaaat tttgctctta
     4141 aaaaaacagg cttcagcttc atgagaagat gatgatagtt tgtgtctttt tcaatgattt
     4201 caattgcaaa tgcgtgtgcc agctcatgca atccaacatt gcgcccatca tttgaatcgc
     4261 gcaaaccaat ctcaaagtct tcccatgaca ggcatatcat gccgtttaca tctacttcac
     4321 ccttgtattg acgtttggtg cgtgggtcga aatagtgggc cggaaagatt tttatgttgt
     4381 tcagatatcc gagcaggctt ttctcaagac caaaagtgat ttgtacaaat gttgctgaaa
     4441 tcaatattac atgtcgtggc ccgagtttca tgcctgcctg cggataaaaa acggtactca
     4501 tgttgaattt tattacacgc cgtataaaga tgcgtttgtt ttcggtggac aacgtcttgt
     4561 aataatacaa ttcattttcc agaattttgt gaatgtctgc ttcgtgtcta tgtagccgta
     4621 tatgtgttcc ctggccggct tcgggatgtt ccgatgtctg tagaaatgta gagaagcgta
     4681 ttttgatata cagcatccct gcgaaaatta ttataaaaac gattattgtt cctgtttctt
     4741 ccattctgta atcctatatc aatgaatttc tgaagatgtc agggaaatgc tctttcagaa
     4801 tttcgatatc ggagggaaca tcaacagaat ggccacggta gcgggtttca attattctta
     4861 tggaataagc attttccagc caacgcaatt gttcaagcga ttcggcctgt tcaagccggg
     4921 atggctgcag tttgattagt tgagaaagtg ttttgatgcg ataggcataa acgcctatgt
     4981 gttgccggaa aggaaactgt tcgaacagaa atttgtcgcg gcgtggattc cggataaaag
     5041 gaatgatgga gcgactgaag tataacgcgt tgttggctgt gtttacaaca actttcatac
     5101 agttttcgga gaaaagggct tcgtcggaat caaaggggcg gacaagggtt gcaatattaa
     5161 cttgcgggtc tttgaatgct gctgccagct gatcgatgat tacaggatcg ataaatggct
     5221 catctccctg aatattgatg attacagtgt cgtcgtcgtt ggcatcggcg cccgaaagaa
     5281 ttgcagcttc aaaacagcgg tcggtgccgc tcggatgatt ggatgcagtc ataatggctt
     5341 tgcctccgaa aaattcaacc ttctgaaaaa tccggttgtc atcagtggca acaaatacct
     5401 gcgacagtga atcagctttt gatgcatttt cgtaaaccca ctgtaccatt ggttttcctg
     5461 caataatagc aagtggcttt ccgggaaacc gaagcgaagc gtagcgtgca gggatgattc
     5521 cgatgaattt catgttagaa aagtccgaat aattcagcat ttattttttc gatgataacg
     5581 cccagatctt ccttcttttc atggaatttg actttctcca cgtcaataat cagaagtttg
     5641 ccgtctttgt attcattgat ccaactttcg tagcgttcgt tcagactttt gaggtaatcg
     5701 agtcgtatcg aggcctcgta atcccgtccg cgtttctgaa tctggctcac caatgttgga
     5761 acatcggctc gcagatagat aagcaggtcg ggtgctttga tgaaggatac cattaattcg
     5821 aaaatggatt gatatgttgc aaaatcacgc gacgacatta aacccatatc gtgcaggttg
     5881 ggcgcaaaaa tgcaggcgtc ttcatagatg gtgcggtcct gaatcacatc tttggcctgt
     5941 cggtgcaatt ccacaatttg cctgaaacgg ctgttgagaa aatatatctg cagattgaac
     6001 gaccaccgtt tcatgtcttc ataaaaactg gtgagatatg gattggtgtc aacatcctcg
     6061 tattggggag catatttgta atgtttggcc agcagccctg tcagggtggt cttgccgctt
     6121 ccgatgtttc ctgcgattgc tatatgcata aatggtgttt tttttatcag ggtgctaatt
     6181 tcagattttt attttaaaaa agaaaaatta aatcacttta attgttactt tttctgaaca
     6241 atgtgatttt tttttcattg agaattgcca tccattgtga tgaaacagca attccattga
     6301 ttttttcaca accggtttca atgattgctt ctttgtgcaa ttgtgtttga tatgcatgca
     6361 gtgtgtcgtt gagtacataa tacaacacat ctccatcgag cgaccagtaa tcggcttttt
     6421 cattaaaatg cattctgagg tttccaaacc gatcaaagat gtaagcagaa cctccttttt
     6481 gagccagtaa aatgttggtg cccgactcct gaagaatggt tggcgtgaaa tttttgaaat
     6541 caagcagtag ccctgcattg gttttcgatg taatggtaag ttgctccgaa agacgtacca
     6601 gatccatgtt cagcgctgaa agcgtccaga atgcgttgtc gtacgaactg cacatcaatg
     6661 aaatgtcgcc ggtttcaagc atggaaaaat cgatattgtt tattttggca aagtccgagc
     6721 tataaatgtc tgctatctgc aggtcgcggt tgaatacaat aattttgtat ggatccgaag
     6781 catcaatctg ccagaaactg cttttgcttt tccggccggg aatggctaca ggagtcccgg
     6841 tggtgtcaaa tttcaacaga ctgccgtttt cgaaaacaac atatacagag cccatcggat
     6901 cgacagaaaa agcagcagct tgttcggaga gtttgtattc gcgcactgga tgcggacttt
     6961 gtgaataagt caacatgcac atgtgtaaaa acaatatgct gaaataaaaa tgtttcattg
     7021 attgaatgag tcaaagataa tattttttaa gtcaaaaaat gaattgacta ttgtattgtt
     7081 ttttttgtat ttttgcaccc cgcgtcaacg acatcagaca aacgcaaata ctttttacag
     7141 ttcaattttg catttcgtat ttcgtatttc tgatctcgta tttcacttgc ccgagtggcg
     7201 gaattggtag acgcgtcggt ctcaaaaacc gatgaggtaa cactcgtgcc ggttcgatcc
     7261 cggcctcggg tacaaaa
//
LOCUS       DAOPKE010000124         7169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_11091_length_7169_cov_10.880377, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000124 DAOPKE010000000
VERSION     DAOPKE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11091_length_7169_cov_10.880377"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..260)
                     /locus_tag="PKY63_11635"
     CDS             complement(60..260)
                     /locus_tag="PKY63_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01311.1"
                     /translation="MKKLLLITGLALALASCGGKSDKAMTPEEENQFATEEVKAIDSS
                     VTSVVDSVDAKEAKVNELLKGI"
     gene            complement(287..952)
                     /locus_tag="PKY63_11640"
     CDS             complement(287..952)
                     /locus_tag="PKY63_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01312.1"
                     /translation="MKKLLLITGLALALASCGGKSDKVENQVHDNNEKTNNKIDLISE
                     IEKIVNSANSLNAKLDQEETNVYDILGKCDKNIHEEKNAAHPLGMQLYYPERVSETSS
                     KVDIKQMTPIKRGYDIYGNKYMSDEEIEKEYGKYYKLDEAQWLANQQKERVRALIEKC
                     NQAKENNQNLFQGNKISKNEFDKNVDLINSAKKTSSNLNEQITQLQTQIDELKELTRT
                     STN"
     gene            complement(1035..2555)
                     /locus_tag="PKY63_11645"
     CDS             complement(1035..2555)
                     /locus_tag="PKY63_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPF01313.1"
                     /translation="MIKVLDIKTIREADQYTIEHEPVSSLDLMERAGGLCFEWIKEKF
                     PDTKQHFIVLAGSGNNGGDGLVIARMLHDSGYTVDVSLIYLSDKLSPDCKRNLDRLKK
                     KLKVNVVEKAEDIPALPAESIVIDALLGSGINRAPEGIVLNVIERINTSANTVISIDL
                     PSGLFADASSHNHSKKVVQADFTLTIGAPKLALFMPENGIYSGEWHLVRIGLHEEFMQ
                     QAPSKYFYLQPADFYGIISPRKTFSHKGTYGHALIMAGSKGKTGAAVLAALACLRSGC
                     GLTTVHCPSDSLKIIQTTAPEAMATFDKNTDCISELPDLSPFSAIAFGPGCGQNKATA
                     TALKLLIQESKVPLLIDADGLNILAANKTWLGFLPENTILTPHPGEFNRLFGESKNDF
                     ERLQKAIGSAAKYKCIIVLKGAYTAIISPSGQVFFNCSGNPGMAKGGSGDVLTGLIAG
                     FIARGIPALNAALTGVYLHGLAGDLAADSLTQEAMNAGDLIGFLPKAWAQILQNQE"
     gene            complement(2559..2903)
                     /locus_tag="PKY63_11650"
     CDS             complement(2559..2903)
                     /locus_tag="PKY63_11650"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HPF01314.1"
                     /translation="MDNQTPDNSPFLKPEIPNASSAELIGILSIVFTFFFTLAGLVLG
                     IVAIVQARKAEETFNANPGMFNPYSLNKVKTGKTCGIIGVVLSGLIIVAAIILVVVLI
                     SVFAHYGTHSCH"
     gene            complement(2907..4187)
                     /locus_tag="PKY63_11655"
     CDS             complement(2907..4187)
                     /locus_tag="PKY63_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPF01315.1"
                     /translation="MTQNHSSKSQVFALLIVGILFFIFGFVTWLNGTLIPFLKIACEL
                     STQQAFFVATAFYFAYFVMAIPVSFLLRTNGFVNSMSMGLIIMMFGTLLFIPAAYSRE
                     YWIFLVALFIQGTGLTLLQTAVNPYVTLLGPVESAAKRISIMGVANKLAGAFSGIILG
                     SILLAKSDTEIKTDLNLLDSAQKALYLDDMAQKVVGPYLLMAGVLLLLAMMVRYARLP
                     EISGDEPDAKFSAKGIPTYAWMGVIAIFFYVGAEVVVGDSVILYGRYLNPDPAFINIF
                     GYEINILNPSYFTSYVMTGMIIGYFIGIALIPKVISQQKALTLFSISALLFTTLALLS
                     SGIGSLFFIALLGISNAVMWPAIWPLAIEKTGKKTPIISALLIMGIIGGAIMSPVFGK
                     LADLFNMHQAYWLLIPCYLFVLFFATIGHKINKH"
     gene            4358..>7169
                     /locus_tag="PKY63_11660"
     CDS             4358..>7169
                     /locus_tag="PKY63_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPF01316.1"
                     /translation="MKKIHFLFSFLMMIIWVSPVFSQISQGGTPRSFSIQLDSKQAAQ
                     VPVIQMIDVDVDSLRAEDAINDPLKDRPWRFGENLFVDIDVKSDGHVTLMPNGDKIYR
                     VSIYSPEAVSLNLTFDRYRLPAGADLFIYNGDQSEVLGAFNEQNNQDDGIFASALVTG
                     DLITIEYFEPASVAFPGELHLFRVTHGYRGIKDFTKAFGQSGYCNVNVACPEGVGWEN
                     EINAVCMLVSGGSGFCTGSLVNNTANDGTPYILTADHCYSDPTSWVFYFNWQSATCTN
                     PGSSPSYDQISGAQLRARNTPSDFCLVEMNSTPPSSYGVYYAGWNRATDATISGTSYG
                     IHHPSGDIKKISWTTIPLTTTSYGQTTSPGDGSHFRVTSWADGTTTEGGSSGSALFDS
                     NHRIVGQLHGGSAACGNTLSDWYGRFSVSWTGGGSNTTRLSNWLDPMGTAPTTLDGYD
                     PNTPAFALDAQVSAIQQPTGTTCGSISITPTVTLRNAGSTTMTSATVYYRIDGGTPVS
                     QTWSGSLATNSATTITFPATTSTYGTHTIESWSASPNGGTDANTANDTATASFELSSG
                     IYSVPFVELFNDGVPPTCWTSYAGTNGLGTVETWGTTTDYQYEGTGAAIIYYENVTGG
                     LAEDWLVTPQIQLQNNSTLTFYHRQGYTDNYNSTYSVKVSTTSQTSHASFANVQTWDE
                     TGLTTAYTKKTIDLSAYDGQNVYIAFVFSNDNGDAWLIDSLKVTGTCTTPVAAFAASS
                     TSTCTNSAITLNDGSTGTPTSWAWSISPATFNYVGGTNATSQNPQVEFTVAGSYTIQL
                     TATNACGSDVENKTNYVTVIAPPAQPSAIAGNANPCAGVTGLTYSVTNVGGVTYNWSV
                     PTGWTITGGAGTNAITVTAGTTAGTISVTPSNGCDGIPQTLAVSVNTVPAQPSAINGQ
                     IGPCQSASETYSVTNVAGVTYTW"
BASE COUNT         1928 a         1580 c         1566 g         2095 t
ORIGIN      
        1 atcattgcaa atgcgatggc aagcataaat gaaaatagtt ttttcatgat gttttctttt
       61 taaattcctt taagtaattc gttcactttt gcttctttgg cgtcaacgct gtcaacaacg
      121 ctggtcactg atgaatcaat ggctttgact tcttcagtag caaactgatt ttcctcctca
      181 ggagtcatgg ccttgtcgct tttgccaccg catgatgcga gtgctaaagc aagccctgtg
      241 attaaaagta gttttttcat gtctggttta ttttatttgg gttgatttaa ttggtggaag
      301 tccttgttaa ctctttcaat tcatcaattt gtgtttgaag ctgggtgatt tgttcattta
      361 aattgcttga cgttttcttt gctgaattaa tcaaatcaac atttttgtca aattcatttt
      421 ttgatatttt attaccttga aacaaattct gattattttc ttttgcctga ttacactttt
      481 caatcaatgc ccgcactctt tccttttgtt gatttgccaa ccattgagct tcatcaagtt
      541 tgtagtattt gccatattcc ttttcaattt cttcatcact catatactta ttgccataaa
      601 tatcatagcc acgcttaatt ggagtcattt gtttgatatc tacttttgag cttgtttctg
      661 aaactctttc cgggtaatat aattgcattc caagtggatg tgctgcattt ttttcttcgt
      721 gtatattctt gtcacattta ccaagaatat cataaacatt tgtttcctct tgatctaatt
      781 tggcattcag tgaattagct gaattaacta ttttttcgat ttcactaatt agatcaattt
      841 tattatttgt tttttcatta ttatcatgga cctgattctc tactttgtcg cttttgccac
      901 cgcatgatgc gagtgctaaa gcaagccctg tgattaaaag tagttttttc attctggttt
      961 attttttatt ttggtctgca aagttacggc ggtttttaag aaaccggatt gattttctgg
     1021 aatttaaccg tttattattc ctgattctga agaatctgag cccaggcttt tggcagaaac
     1081 ccgatcaggt ctcctgcatt catggcttcc tgtgtcaaag aatcggcagc cagatcgccg
     1141 gccagaccat gcagatacac ccctgtcaaa gccgcattca gagcgggaat tccgcgggcg
     1201 ataaatcccg cgataagacc ggtcagtaca tcgccgctgc cgcctttggc cattccggga
     1261 ttgccgctgc aattgaaaaa cacctgacct gaaggagaga taatggctgt atatgcgcct
     1321 ttcagaacaa tgatgcattt atattttgct gccgatccaa tggctttctg caatcgctca
     1381 aaatcatttt tactttcacc aaacagtctg ttgaattctc ccggatgcgg cgtcagtatt
     1441 gtgttttcgg gcaaaaagcc aagccatgtt ttattggccg ccagtatgtt caatccgtct
     1501 gcatcgatga gcaacggcac tttcgattcc tgtatcaata atttgagggc tgttgcagtg
     1561 gccttattct gcccgcatcc ggggccgaaa gcaatggctg agaatggaga aagatccgga
     1621 agttccgaaa tgcaatccgt atttttatca aaggttgcca tggcttcagg agctgttgtc
     1681 tgaattattt tcaacgaatc cgacggacaa tgaacagtcg ttagcccgca gccggagcgc
     1741 aggcaggcca gtgcagccag aacggcggcg cctgtttttc ctttactgcc ggccatgatc
     1801 agggcatggc cataggtgcc tttgtgcgaa aaagtttttc tcggagaaat aatgccgtaa
     1861 aaatcggctg gctgcagata gaagtatttt gatggcgcct gttgcatgaa ttcctcgtgc
     1921 agtccgatgc gaactaagtg ccattcgccg ctgtaaatgc cattttcagg catgaacaga
     1981 gccagttttg gtgcgccgat tgttagtgta aaatctgcct gtacaacttt ttttgaatga
     2041 ttgtgggagc tggcatcagc aaataatccc gatggcagat caattgaaat tactgtgttt
     2101 gctgatgtat tgattctttc gatcacattc agcacaattc cttccggcgc ccgattgatg
     2161 ccggaaccca gcagcgcatc gattacgatg ctttctgccg gaagtgcagg gatatcttct
     2221 gctttctcaa caacgttgac tttcagtttc ttttttagtc tatccagatt tcgcttgcaa
     2281 tcagggctaa gtttatccga cagataaata agactgacat cgacagtata tccgctgtca
     2341 tgcagcatgc gggctattac cagtccgtcg ccgccattat tccctgatcc ggccaaaacg
     2401 ataaagtgtt gttttgtatc aggaaatttc tcttttatcc attcaaaaca taatccccct
     2461 gcgcgttcca tcaggtcgag cgaagaaact ggctcatgct caattgtgta ttggtcagct
     2521 tcgcggatgg tttttatatc aagtacttta atcatggatt aatggcagga atgagttccg
     2581 taatgcgcaa aaacagaaat caaaaccaca actaaaatta tcgcggcaac aatgataagt
     2641 ccgcttagca caactccaat aatgccacag gtcttgcccg ttttgacttt gttcagcgaa
     2701 tacgggttga acatgccggg attggcattg aacgtttctt cggctttgcg ggcctgaaca
     2761 atagccacaa ttccaagaac cagtcccgcc agggtaaaga aaaaagtgaa tacgatagac
     2821 agaatgccta tgagttcagc cgatgaggca ttgggtattt caggctttaa aaaaggggaa
     2881 ttgtcgggtg tttgattgtc catttttcag tgtttattga ttttgtgacc gatggttgcg
     2941 aagaaaagca cgaacaaata acatggaatc agcagccagt atgcctgatg catattgaat
     3001 aaatcggcaa gtttgccaaa aacgggactc atgattgctc cgccaatgat acccattatg
     3061 agtaatgcgg aaataatggg tgtttttttt ccggtttttt caatcgccag aggccagatg
     3121 gcaggccaca ttacagcgtt tgatattcca agcagtgcaa taaaaaacaa cgatccgatt
     3181 ccgctgctca gaagtgccag cgtggtgaat aacagcgcac ttattgaaaa aagcgtcagg
     3241 gctttttgtt ggctgattac ctttggaatg agagctattc caataaaata cccgataatc
     3301 atacctgtca tcacatagga ggtgaagtaa cttggattga gaatgttgat ttcgtagccg
     3361 aaaatattta taaaagccgg atccggattg agatagcggc catataaaat aacactgtca
     3421 ccaaccacaa cttccgctcc gacatagaaa aatatagcaa taacgcccat ccaggcataa
     3481 gtgggtattc ctttggccga aaattttgca tcgggctcat cgcctgaaat ttccggcagg
     3541 cgggcatagc gaaccatcat agcgagcagg agcagaacgc cggccatcag aagatagggc
     3601 ccaacaactt tttgcgccat gtcatccaga tagagcgctt tttgtgctga atcaagcaga
     3661 ttcagatccg ttttgatttc agtatcggat tttgcaagca ggatagaacc cagaattatt
     3721 ccgctgaaag cacctgccag tttgttggca acccccatta tgcttattcg tttcgcagca
     3781 ctttcaaccg gaccaagcag ggttacatat ggattgaccg ctgtttgcag caaggtgagt
     3841 cctgtaccct gaataaaaag ggcaacaagg aatatccaat attcacgcga atatgcggcc
     3901 gggataaaca ataatgttcc gaacatcatg ataatcaaac ccatcgacat cgaattcaca
     3961 aagccattgg tccggagaag aaatgaaacc ggaatggcca ttacaaagta ggcaaaataa
     4021 aaggcggtcg cgacaaaaaa agcttgctgg gtgcttagtt cacaggcaat ctttaaaaag
     4081 gggattaatg tgccgttcaa ccaggtaaca aagccaaaaa taaaaaacag gatgcctaca
     4141 ataagcagcg caaaaacctg acttttggat gaatgatttt gtgtcattga aagggggatt
     4201 tttttacaaa gatgcaaaat attcccattt tgctgactag ttttgttcac atcgtagggg
     4261 aaaagaattt ttgtgaataa aatactgtga aatgtgagtt aaaaaattga gagagtgtag
     4321 taaagttgta ttttagcgac tcaaaaaccc ccaaaaaatg aaaaaaatcc actttttatt
     4381 ttcatttctg atgatgatta tatgggtttc tcccgtattc tcacagatca gtcagggcgg
     4441 aacgccccga tcgttttcga ttcagcttga cagcaagcaa gctgcacagg tcccggtaat
     4501 tcagatgatt gatgttgatg tggactcact ccgggccgag gatgccatca atgatccctt
     4561 aaaggatcgc ccttggcgct ttggcgaaaa tttatttgtt gacattgatg taaaatcaga
     4621 cgggcatgtg acactgatgc ccaatggcga caagatttac cgcgtttcca tttattcacc
     4681 ggaagcggtt tcacttaatc tgacttttga ccgttacaga ttgccggcag gagctgattt
     4741 gtttatttac aatggtgacc agtctgaagt tttgggcgca tttaatgaac aaaacaatca
     4801 ggatgatggt atttttgcct cggcattggt aacaggtgat ctgattacca ttgaatattt
     4861 tgaaccggct tcagttgctt tccctggcga attgcatttg ttccgtgtta cccacggata
     4921 tcgtggcatt aaggatttta ccaaagcatt tggacagtct ggttattgca acgtcaatgt
     4981 ggcttgtcct gaaggcgttg gttgggaaaa cgaaatcaat gctgtttgca tgcttgtttc
     5041 cggtggttcc ggattttgca cgggatcact ggtcaacaat acggcaaatg atggcacccc
     5101 ttatattctg acagcagacc attgttattc agatcccaca agctgggtgt tttatttcaa
     5161 ctggcaaagt gcaacctgca caaatcctgg ttcatctcct tcttacgatc agatatcggg
     5221 agcgcaactc agagccagaa atactcccag cgatttttgt cttgttgaaa tgaattcaac
     5281 tccgccttcg tcatatggtg tgtattatgc cggatggaac cgggctactg atgcaactat
     5341 ttcaggaacg tcttatggta ttcaccatcc cagtggcgac atcaagaaaa tttcatggac
     5401 aaccattcca ttgactacaa cttcgtatgg acaaactacc agccctggtg acggctcaca
     5461 tttcagggta acctcatggg cggatggaac aactactgaa ggtggatctt ccggttctgc
     5521 tttgtttgat tcgaatcatc ggattgtagg gcaacttcat ggtggatctg ccgcctgtgg
     5581 taacacatta agcgactggt acggtcgttt cagtgtttca tggaccggag gtggctcaaa
     5641 tactacccgt ttgagcaact ggcttgatcc aatgggaact gctcccacta cgcttgatgg
     5701 atatgatcca aatactcctg cttttgcgct tgatgcgcag gtgtctgcta ttcagcaacc
     5761 gaccggtacc acttgtggat cgatcagtat aacacccact gttactttgc gcaatgccgg
     5821 ctcaaccaca atgacctctg ctaccgttta ctacaggatt gatggaggaa caccggtgtc
     5881 gcagacatgg agcggctcac ttgcaacaaa ttcagctacg acaatcactt ttccggctac
     5941 aacatcaaca tatggcactc atacaatcga gtcctggtct gcttctccaa atggtggcac
     6001 tgatgccaat acggccaatg acactgcaac ggcttcgttt gaattaagtt cgggcattta
     6061 ttcggtaccc tttgttgaac ttttcaatga tggtgtacca cctacatgct ggacctcata
     6121 tgctggaacc aacggacttg gcactgtgga aacctggggc acaacaacag actatcaata
     6181 tgaaggaacc ggagctgcaa taatttatta tgaaaatgtc acgggtggcc ttgctgaaga
     6241 ttggcttgtt acacctcaga ttcaactgca gaataattcg acgcttacat tttatcacag
     6301 acagggttat acagataatt acaattcaac ctattccgtg aaggtatcca caacttctca
     6361 aacttcccat gcttctttcg ccaacgtcca gacttgggat gagacagggc taacaacggc
     6421 ctataccaag aaaacaatag atctgagtgc ttatgatggt cagaatgttt atatagcttt
     6481 tgttttttca aatgataatg gtgacgcctg gcttattgac agtttaaagg taactggtac
     6541 ctgtacaact ccggttgcag cttttgccgc atcgagcacc agtacatgta ccaactcggc
     6601 gattaccttg aacgatggtt caactggtac ccccacatca tgggcgtggt caatctctcc
     6661 ggctactttc aattatgttg gcggaacgaa tgcgacaagt cagaatcctc aggttgagtt
     6721 tacagttgcc ggatcatata ccattcagct cactgcaacc aatgcctgcg ggtcagatgt
     6781 tgaaaacaaa accaactatg ttaccgttat tgcacctccg gcacagccca gcgccattgc
     6841 cggcaatgct aatccttgtg caggtgtaac gggtctgaca tacagtgtta ccaatgttgg
     6901 cggtgttacc tataactggt ctgtgccaac cggttggaca atcactggtg gtgcagggac
     6961 taacgctatt acggtaacag ccggaaccac agccggaacc atttcagtaa ccccaagcaa
     7021 cggatgcgac ggcattccgc aaacgctggc tgtatcagtc aacacggttc ctgctcagcc
     7081 atcggcaatc aatggacaga tcggaccatg ccagtcagcc agtgaaacat attctgtaac
     7141 caatgttgcc ggggtaactt atacctgga
//
LOCUS       DAOPKE010000125         7023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_11418_length_7023_cov_10.153559, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000125 DAOPKE010000000
VERSION     DAOPKE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11418_length_7023_cov_10.153559"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKY63_11665"
     CDS             complement(<1..1022)
                     /locus_tag="PKY63_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPF01317.1"
                     /translation="MKNYANTSDKHLHIVSFDIPYPANYGGAIDVYYKLKALHDAGVK
                     VILHNFQYGGRKRDSALNALCNKVYYYQRNNNKTGILSSKPFIVVSRSSDELLKRLAA
                     DQHPVLFEGIHTCLYLSHPSLSHKKRFVRTHNIEHEYYNGLAQAESDPLKVLYFQSES
                     RKLKRFEKNLSQATGILTISKNDELHFSQINPNICTVSAFHPSEKINIRHGLGSFALY
                     HGSLDVSENNRAALYLVNEVFSDLDYPLVIAGNKPSKELRAAVKKHNHIELKDNLGTD
                     DFSQLVAEAQINILPTFQATGIKLKLLLALHEGRHLLVNTPMVENTGIEDLCHIADSP
                     AEMKTM"
     gene            complement(994..2109)
                     /locus_tag="PKY63_11670"
     CDS             complement(994..2109)
                     /locus_tag="PKY63_11670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF01318.1"
                     /translation="MRIISAVINDLVTDQRVHRSCMLMHDMGYDVTLIGRRLPDSLPM
                     PERPYRCIRMRLPFRTGPAFYAVFNIHLFFRLLFSRFDAVWANDLDTLWACRLASKLK
                     RKKLIFDSHEYFTGVPELEGRPRVQKFWKRIERRIVPKLPFMITVNDSIAELFRNEYN
                     IPVLVVRNVPLRNHLRSSKSRAELGLPDDKKILLVQGRGINIDRGVEEIVLAMKHIEG
                     ALLLIIGGGDVFPVIKRMIAEENLSDKIMTLPPMKREDLMQYTMAADIGLTLDKDTNI
                     NYRYSLPNKIFDYIQAGLAVMCTNLVEVAKIVNTYEVGAVTQSSEPSALATTLKMMIS
                     DEARLNEWKKNASKVSELLCWENESKELNEKLREYLG"
     gene            2401..3216
                     /locus_tag="PKY63_11675"
     CDS             2401..3216
                     /locus_tag="PKY63_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPF01319.1"
                     /translation="MNTAESIRQHAYNIRINIIRSLEAAGSGHLGGSLGLADVFSVLY
                     FHTMKHRPQEPEWVERDRLILSIGHVAPVLYAALAEAGYFHAEELLTLRKLGSRLQGH
                     PGRDHGLPGIELSAGSLGQGLGVAVGTALGLRQQQNNARVFCILGDGELQEGSVWEAA
                     MSAAHYKLTNLMAIVDRNGVQIDGPTEKVMSLEPLKQKWEAYGWHVVETDGHDIEELI
                     DAFANPHLKRPTAIIVQTVMGRGIPEIENDYRWHGKAPDAEQAKRFVDSLRNV"
     gene            complement(3632..5872)
                     /locus_tag="PKY63_11680"
     CDS             complement(3632..5872)
                     /locus_tag="PKY63_11680"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPF01320.1"
                     /translation="MKAYAVMAAGFVLLITLFQSASAQKDSLMVTQDYITMKYGFIDE
                     NGTIIIPAIYNTAYDFSEGYAYVETDEKTGFIDKTGTIKILVKFDAYRYVTDGLIFGE
                     NENGSGCIDISGNIVVPFIYEYCMPMTEGMILYGDEGKYGLMNKEGKMITSARFPYIS
                     PFSESMAYFTENEKIGYINTAGQVLIQPKYYDAGHFREGLGAVMLNGKWGFIDKNGKE
                     IIPIQFDAVSEFSEGRAAFTDDELAGFIDKKGNKITQPRYKMYSLFENGIALVCSDLS
                     WGVIGLNGEEIIPEHYTEIVRAGKTHLIVYDETKLKYGLFNKTGKEILPVIYDWIELR
                     SDDIFYVSLNNKTGLVDGNGKEIIQVVNESVSPFYGDICIVYFDDQPFIANKKGETII
                     PAGKYQSLYYYGRNLIQVSNGEHIGFIDCLGNEIIPVKYDFCGSFTESEVTFFNDGEK
                     YSLINYKGEVIDVEYDEINDFYGGLASVSLNQKYGYINTKGEEVIPLRYDYADAFSYN
                     ITRVQYNGKYGLINQKDSIIVPFIYDEMEPQYSPYEDSYNYNPVWYKVRKENRYGFID
                     LDGKVIIPVEYSYIADFNEMQIALATKNNKTGVINAENIVMIPLIYDEIGIFRNGAAK
                     ANKEGKWGIIDSANNVILDFKYDFVNNLSESMAAVENDGKWGFCNQHGYEVCKIKYEA
                     VENFNEGYAAVKYNGKWGFIDKDGKEVVPCIYTSVTNFNGGFCIAVAGKKTFSIEAGM
                     FTRTKM"
     gene            6052..6603
                     /locus_tag="PKY63_11685"
     CDS             6052..6603
                     /locus_tag="PKY63_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01321.1"
                     /translation="MKKTEKKPVKKPATKKEAPKKEKDLKKKTAATKAKEPVKPKTAV
                     KDKAAVKTKTKAKVEVKDKTKVKDKTKVEVKTKEQSSKTNKKNPLEEQLRPGRNKPCS
                     SKSITPSKDAGKKVRSSKEKVEVKMKDEDKIKDIVEKKVEEPETEEYVSKEMTNEVIE
                     EKTPKAPKKKKKKGAENQLALDF"
BASE COUNT         1984 a         1527 c         1475 g         2037 t
ORIGIN      
        1 atcatggttt tcatttcggc gggactatcg gcaatatggc acagatcctc aattccggtg
       61 ttttcaacca tgggtgtatt caccagcaga tgacgtcctt cgtgcaatgc cagtaatagt
      121 tttaatttaa tgccggttgc ctgaaacgta ggcagaatgt tgatctgcgc ctcggccacc
      181 agttgcgaaa aatcgtcggt gcccagatta tctttcagct caatatgatt gtgctttttc
      241 acggcggcac gcagttcctt gctcggttta tttccggcaa ttaccagcgg ataatcgagg
      301 tcactgaaca cttcattcac cagatacagc gctgcgcggt tgttttcgct cacatcgaga
      361 ctgccgtgat acagcgcaaa agatcccagt ccatgacgga tgttgatttt ctcagatgga
      421 tgaaacgcgc ttaccgtgca gatgttcggg ttgatctgcg aaaaatgcag ctcatcgttt
      481 ttcgatatag tgagaatacc ggtggcctgc gacagatttt tttcgaagcg tttcagtttg
      541 cgtgattcgc tttgaaaata caacactttg agcggatccg attcggcctg tgcaagcccg
      601 ttgtagtatt cgtgctcaat gttgtgtgtg cgcacaaagc gctttttgtg cgataaagac
      661 ggatgcgaaa gatacagaca ggtatgtatt ccttcgaaca aaaccggatg ctgatcagct
      721 gcaagccgct tcaacagctc gtccgaagac cgggaaacta caatgaacgg tttagatgat
      781 aatattccgg ttttattgtt gttgcgctga tagtaataca ccttgttgca aagtgcgttc
      841 agagctgaat cgcgcttacg tccaccgtat tgaaaattgt gcaggataac tttcacaccg
      901 gcatcgtgca gcgctttaag tttataatac acatcaatgg caccgccgta attggcaggg
      961 tagggaatat cgaaggaaac gatatggaga tgtttatccg aggtattcgc gtaatttttc
     1021 attcagttct ttggattcat tttcccagca cagaagctca gatactttcg acgcattttt
     1081 cttccattcg tttaaacggg cctcgtccga aatcatcatt tttaatgttg tggccagggc
     1141 tgatggctct gaactttgtg tcacagcgcc gacttcgtat gtattgacaa tttttgccac
     1201 ttcaaccaaa tttgtgcaca tcaccgccag acctgcctgt atgtaatcga atattttatt
     1261 tggaagactg taccgataat taatgtttgt atccttatcg agtgtgagtc cgatgtcagc
     1321 agccatggta tattgcatca ggtcttcgcg tttcatgggc ggaagcgtca ttattttatc
     1381 agaaagattt tcttcagcaa tcattctttt gattacaggg aatacatcgc ccccaccgat
     1441 aatgagtagc agtgcgcctt cgatgtgttt catggcaagc actatttctt cgacgccccg
     1501 gtcgatgttg ataccacgtc cctgtacaag cagaattttt ttatcatccg gaaggccaag
     1561 ctctgcgcgt gatttcgaag atcgcagatg attgcgcagc ggcacattgc gcaccaccag
     1621 caccggaata ttatattcgt ttctgaacaa ttcggcaatg gaatcattta ccgtaatcat
     1681 aaacggaagc ttaggcacaa tgcgcctttc gatgcgcttc cagaatttct gcacccgcgg
     1741 tcttccttcg agttctggca cgccggtgaa gtattcatga ctatcgaaaa tcagtttttt
     1801 ccgtttcagt ttcgaagcca gacggcaggc ccagagcgtg tctaggtcgt tggcccatac
     1861 tgcgtcgaaa cgtgaaaaaa gcaggcgaaa aaacaaatga atgttgaata ccgcgtaaaa
     1921 cgcaggcccg gtgcggaaag gcagtcgcat acggatgcat cggtatggtc tttcgggcat
     1981 gggcagactg tcgggcaggc gcctgccgat gagtgtgaca tcatatccca tgtcgtgcat
     2041 cagcatgcag gagcgatgta ctcgttggtc ggttaccaga tcgtttatga cagccgaaat
     2101 gattctcatt gcccacaaaa ttagccaaaa tatccaaact ataatcgggg ctgtctcaaa
     2161 acaagcatag caaaacgaca ttccatagta caacaagctt ttttatattt tagcttttga
     2221 tgcagaacat ggaatagtcg agcaaaaaca atgcttccgg cacaaccgac tcttccatgt
     2281 attccattgc attccagact atggaaggtc ggctcgcatg aaaaagttgt tgtttttgcc
     2341 ggaacagatt cttttgagac agactcggat atttattatt tttgcaacca atcattttgt
     2401 atgaacaccg cagaatcaat ccgtcaacac gcatacaaca tacgaatcaa catcatccgg
     2461 tcgctcgaag cagccggcag cggccatctc ggaggatcgc tcggactcgc cgatgtcttt
     2521 tctgttttgt attttcacac tatgaagcac aggccgcagg agccagaatg ggttgagcgc
     2581 gaccgtctga ttctcagtat cgggcatgtg gcgccggttt tgtatgcggc tctggctgaa
     2641 gccggatatt ttcatgcaga agaattactc acgttgcgca agctcgggag ccgcctgcag
     2701 ggtcatcccg gacgcgatca cggattaccc ggaatcgaat tgtcggctgg ttcgcttgga
     2761 caaggtttgg gtgtggccgt cggaacagcg ctcgggctgc ggcaacagca gaacaatgcc
     2821 cgtgtatttt gcattctcgg tgacggtgaa ttgcaggagg gcagtgtttg ggaagcagcc
     2881 atgagtgcgg cacattataa gctcaccaac ctgatggcca ttgttgatcg caacggagta
     2941 cagattgacg gcccgactga gaaagttatg tcgctcgaac cattgaaaca aaaatgggaa
     3001 gcatacggct ggcacgttgt tgaaaccgat ggacatgata ttgaagagct cattgatgca
     3061 tttgcaaatc cgcatctaaa gaggcctaca gcaattattg tccaaacagt gatgggcaga
     3121 ggtattcctg aaatcgaaaa cgattaccgc tggcacggca aagctccaga tgctgagcag
     3181 gcaaagcgtt ttgtcgattc actccgcaat gtgtgaagca caaaccaaaa cgctttgcaa
     3241 gttgaaagtt tgtaaagtta tcaagttcat aaaggtgacg tttgcgcatg gggtatagaa
     3301 gggctgattt ttctccgttc cttgttatat ctttggcgat ggggcgttta tgcgtttatg
     3361 cgatttttga tttgtcacat catcgccata tagcattatc gccaaacaaa gtgcaatctc
     3421 cttctcagag caccgaatct tcggtcggta cattctgctg attactgaca actgaaagct
     3481 gatcctacat tttccgaaat aatctgaaag agttggtaaa acaaagctca ctactcatca
     3541 ctcactactc actactcacc gctcaccgct cactgctcac cacttaccgc tcactgctta
     3601 aaacagactt gctgaaagct gacaacagtt atcacatttt cgttcttgta aacattcccg
     3661 cttcgatgct aaaagtcttt tttcctgcaa cagcaatgca gaatccgcca ttgaaatttg
     3721 ttacagaagt ataaatgcag ggcaccactt cttttccgtc cttgtcaatg aaaccccact
     3781 tgccattgta tttcacagca gcatagccct cattgaaatt ctccacggcc tcgtatttta
     3841 ttttgcatac ttcgtaacca tgctgattgc agaaacccca tttaccgtcg ttttccactg
     3901 ctgccattga ttcagacaga ttatttacaa agtcatattt aaaatcaaga atcacgttat
     3961 tggcagaatc aataataccc cacttccctt ctttattggc ttttgcagcg ccattacgga
     4021 aaatcccgat ttcatcgtaa atcaaaggaa tcattactat attttcggca ttaatcacac
     4081 ctgttttatt attttttgtt gccagcgcaa tctgcatttc attgaaatct gcaatataag
     4141 agtattcaac cggaataatc actttgccat cgaggtcaat aaaaccatat ctgttttctt
     4201 tacgcacttt gtaccaaacc ggattatagt tgtatgaatc ttcgtatggt gaatattggg
     4261 gttccatttc gtcgtaaata aagggtacaa tgatactgtc tttttgattg ataagcccat
     4321 atttgccatt atactgaacc cttgtgatgt tgtatgaaaa cgcatcggca taatcgtatc
     4381 tgaggggtat cacttcttcg ccttttgtat tgatatagcc atatttttga tttagcgata
     4441 ccgatgccag tcctccataa aaatcattga tttcatcata ttccacatcg attacttcgc
     4501 ctttgtaatt gatgagtgaa tatttttcgc catcattgaa aaaagtcacc tcactttcag
     4561 taaaagaccc gcaaaaatca tatttaaccg gaataatttc atttcccaga caatcaataa
     4621 aaccaatatg ttcaccattg ctgacctgaa tcaggttgcg cccataataa tataacgact
     4681 gatattttcc ggcaggtata atggtttctc cttttttatt cgctatgaat ggctgatcat
     4741 caaaatatac gatgcatata tcgccataaa acgggctcac agactcgttt acaacttgta
     4801 ttatttcttt gccgttccca tcaaccaacc ctgttttatt gtttagtgaa acgtagaaaa
     4861 tatcgtcgct gcgaagttct atccaatcgt aaattacagg taaaatttcc tttcctgttt
     4921 tgttgaaaag gccatatttc agcttcgttt catcatatac aatcagatgt gttttcccgg
     4981 cacggacaat ttcggtatag tgctccggaa tgatttcttc tccgttaagt ccgataacac
     5041 cccatgaaag atcggaacag acaagcgcaa ttccgttttc aaataatgaa tacatcttat
     5101 atcggggctg tgtaatttta ttcccttttt tgtcaataaa acccgccaat tcatcatcag
     5161 tgaaagcagc acggccttca ctgaattcag aaacagcatc aaattgtatt ggaattattt
     5221 ctttgccgtt tttgtctata aaaccccatt tcccgttaag catcacagcg cccaaccctt
     5281 cacgaaaatg gcctgcatcg tagtattttg gctgaataag cacttgtccg gctgtattta
     5341 tatacccgat tttttcattt tctgtgaaat aggccatgct ttccgaaaat gggctgatgt
     5401 atggaaatcg tgcactggta atcattttcc cttctttgtt catcaatcca tatttgcctt
     5461 cgtctccgta cagtatcatc ccttctgtca ttggcatgca gtattcgtat ataaatggaa
     5521 caacgatatt cccgcttatg tctatacatc ccgatccgtt ttcattttca ccaaatatca
     5581 aaccatctgt tacataacgg tatgcatcaa attttactaa tatttttata gtgcctgttt
     5641 tgtcaatgaa tcccgttttt tcatcggtct caacataggc atacccttcg ctgaaatcat
     5701 atgctgtatt gtagatggca ggaatgataa tggttccgtt ttcatcaata aatccgtatt
     5761 tcattgtgat gtaatcctga gtaaccatca aactgtcttt ttgtgcagat gctgattgaa
     5821 acagtgttat caataaaaca aaaccggcgg ccatgactgc gtatgctttc attgtatttt
     5881 ttttcaaatg taagaatgtt tcatcgttgt caggaaaata aaattggtaa tattgttgaa
     5941 aaatcctgta aatgctttta atccattatg gatgctgctc taacgttttg tgattatgaa
     6001 agtacatttc gcggattttt gtatttttga ttgcccaaaa atctttatcc gatgaaaaag
     6061 actgagaaga aaccagttaa gaaaccagca acaaagaaag aagctccaaa gaaggaaaag
     6121 gatttaaaga aaaaaactgc tgcaacaaaa gcaaaggagc cggtgaagcc gaaaactgct
     6181 gttaaggata aggctgctgt taagactaag actaaggcta aggttgaggt taaggataag
     6241 actaaggtta aggataagac taaggttgag gttaagacta aggagcaaag ctcaaaaaca
     6301 aataaaaaaa atcctttgga agaacagctc cgcccggggc ggaataaacc ctgcagttca
     6361 aaatcaataa ctccgagcaa ggatgcaggt aagaaagtac gaagctctaa ggaaaaggtc
     6421 gaggtcaaga tgaaggatga ggataaaata aaggatatag tcgagaagaa ggttgaggag
     6481 ccggaaacag aagaatatgt ttccaaagaa atgacgaatg aagtaattga agaaaagaca
     6541 cccaaagctc cgaaaaagaa aaagaaaaaa ggcgccgaga atcagttggc actggatttc
     6601 tgatgatatg atttttgcca cgaagacacg aattattcag caaagtcaac tggcgcacac
     6661 ttcactggtg cggatatgtt atccgtgaca gtgataacaa cagacaccgt tattagtcag
     6721 cgtaaacgta ttaaagacgc gagctgatcc tctttgcttg aataatccgt ctgcaacgca
     6781 gtcgcaatgc gcgggtaggg aaatgcggat tttttgatga cccgatcaat tcaaaatcat
     6841 caggcaaaat acttttcgcg gaatgcagtt gtttgcttca tgaaacgcac ctatcacacc
     6901 aatctcgcga ttgcatacaa ttttggcata tatagcgaca ccgtactttg agcgggcatt
     6961 ttcagaatag gttctgcaag tttttatgtg aaacatgaag cttgaggatt aatcaaccaa
     7021 cga
//
LOCUS       DAOPKE010000126         6963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_11554_length_6963_cov_15.297047, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000126 DAOPKE010000000
VERSION     DAOPKE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11554_length_6963_cov_15.297047"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..428
                     /locus_tag="PKY63_11690"
     CDS             60..428
                     /locus_tag="PKY63_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01322.1"
                     /translation="MYQFIDNNASGSNIYYRVSQTDFDGRSTLLGTTKAECGSDVSVL
                     KVVSVDHKNAGVTLNFSTGKSGMHQILLCDIHGKIIEQTSMVCEPGFNQADFCKKTDP
                     GIYFFRILNSAETVSYKCKL"
     gene            604..3318
                     /locus_tag="PKY63_11695"
     CDS             604..3318
                     /locus_tag="PKY63_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01323.1"
                     /translation="MKKIIRFFKNWDRRILLLAGGGALVVILIVLLIVLKPFSSGKIY
                     QANDVIPDNAFAVLRINNPGEIIHNSSYKNTLSNISFMPAAEAFVYGLMQADTVLSVS
                     ESSKNLLSESQLYISFHPVGKNKTQCLFVLQMPEKTNAKLLYEELNNSGKVVFSEKSI
                     DGNICYTIGKKLFNLFPCNNLLIGSLSTQLLEQSIAASEKKSGISNNEEFVSAQKTAS
                     SDCEINIFVNTPMLYRSLGQLVSGNAYVNLAFLQTVSSWGNLDVKLQDDVMVFSGLFP
                     LSKANASFMSLFDEPANGATQDALSMLPANTAAAFSFGFGHFEKFFSAYAKRLSEDKQ
                     NVYNNEEEIAHLNQQAKTESIEKCFYQHISGQLCVFSTSTTAGREPQVYCAFPVSDGE
                     EVKDCLLDYAADGEWDTAQFRNATLICIPVEYGAFSLFGPLFEPISETCIAVTEHFVF
                     IGNSPEHVKPVLNDVLSGRTLGASEYKQNLTNHIASSWNIFSFLNTSASSYNLETRWL
                     SDTSLIASLNDSHKNKKAGIISMTAARQSDGIVVSGTWFFGKSDSSSSASWATTLDAN
                     ISGRPVILKDFKTGEFWIAVADVYDNVYLLNSDGVIVWKKEVGDAVNDDFQLLWSGNK
                     EKRSMAFSTSDGIYIIGLDGEIRSSFPVSVRAGVASNLLVADYDNNQKYRLIFGDSKG
                     VLNNYTTDGKSTEGWLKPDLKNQHQSAVFFSPLGGSDYIVVASPGGTVQVFDRKGKSV
                     VDIDEIQDNKGFCPLQGGRDVKFWTAFSSDGALISVNHHGDINPLTEKSYGENVLFEA
                     SKNQVLAISAGILYVFNAEGRETSTIKISESAVDYINVISVEDNHYLIFHNSDNMIGV
                     VSQESGFKDFLYKSEASAFACIVVNDKILMITSNEKTIVFEIIAAEKD"
     gene            3576..5171
                     /locus_tag="PKY63_11700"
     CDS             3576..5171
                     /locus_tag="PKY63_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01324.1"
                     /translation="MKFKSLFAAGFLVFVSMFLASCVKEEFDMDTVTATNWNPNVAAP
                     LINSSLSLWDVMNDYDSTDLLVVDSNQFVYLVYEDTVYSESAENLIPVANQTMNYSDN
                     LTLGTMTGDYVFDYDYNFDFALGGGMELDSMVLKAGTLSMAMNSDLSYPATIEMTIPG
                     STLNGAPFAVTFDYSGSGSNTTAPLSGGKFVFDNSVVNNRLAVHFKVTVHANGSANNA
                     TYANFNMNLDGMAFRFLYGYLGQFNLSLNNDTIGIRVYNNNLTGTVNWEDPRLYINVF
                     NGWGLPIRTTVDYMEAIRSNDPPASVIISGAGIPNPWDINYPTAVGSVSTSSFFLDKN
                     NSNIDAALNITPQRILALVSGVTNPNGNVVKNFVEDVSRMAVEARLELPFYGTAEGFV
                     LQDTFDISFGEDLSHVDWIMFKLYTNNMFPVDGTVQIYFCDSLNNRLDSLLNPVDQAL
                     LSAIPGPAPEYEVVSPVAKTVSQTIGGSRIGNLENTKKIVVTAWLDTYNGGTQLVKIY
                     SYYKLDVRLSAQAQLKFNSSEID"
     gene            5186..6646
                     /locus_tag="PKY63_11705"
     CDS             5186..6646
                     /locus_tag="PKY63_11705"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPF01325.1"
                     /translation="MKKTIIAIFVFASGLLSVFPLMAQNDMIIQGMRIIPQSYYSNPA
                     FIPQCRWHIGMPGLSSLYIGGGHNGFNAKNVISVNSLDSVELDIESMIGSLKNHNYVS
                     LEANEELLSFGFKIKDKHYINFAATERAFARISYPKDLIEFAFYGNGALLDQTLDIGN
                     FRLTENHYREFAFGYSYVYDKNWTFGARAKVLFGMSNVNTRRTDITLHTEGEFFDITA
                     TSDILVNTNEIDNMSDTAIDEEKYMMDYLFLTKNKGLGFDIGATYKLDDKITFGLSVL
                     DLGFISWNSNPMNLSSKNSDGSFTFDGVDVSEFMEMGADSFMQNLADTLIDIFEVDTT
                     YDKYKTSLNTRIHASAFYKVTEKDHLSALLRMHFYDRKVHPSLSLAYMRKFGDIWHLT
                     GTYSITNRNYVNFGIGMSLKLGPMQLYVLTDNIFGPLIWNRYTWLEEEEDDFGATYYE
                     TEKLTIPRNWKYMNVHFGLNFVFGCKPPKDLVPILE"
BASE COUNT         1909 a         1277 c         1530 g         2247 t
ORIGIN      
        1 aaaatatttt aaactgctct taatcttgct tttaggcggt tttattcata ggagtaatca
       61 tgtatcagtt cattgataat aatgcctccg gcagtaatat ttactatcgt gtttcgcaga
      121 ctgattttga cggtcgcagc accttgcttg gtacaactaa agcagaatgt ggcagtgatg
      181 tcagtgttct taaagttgtt tcggttgatc acaaaaatgc cggggtaaca ttgaatttca
      241 gcacgggaaa atccggaatg catcagatat tgttgtgtga tattcacgga aaaattattg
      301 agcaaacttc gatggtatgt gagcccggat tcaatcaggc cgatttctgc aaaaaaacag
      361 atccgggaat ttatttcttt cgaattttga attcagccga aaccgtttca tataagtgca
      421 aactgtaacc cccccttata tacagtcaac cggagagtcg aaaggcctcc ggttttttat
      481 aaaatgaatt atttttttgt gtttttaatt tatagggcga tacttaaata attaacaaag
      541 tgttgttgat ggattgattt tgtttaggaa caatgcatcg cagccattcg ttagatttgc
      601 agtatgaaga aaattatccg gtttttcaaa aattgggatc ggcgcattct cctgcttgca
      661 ggtggaggtg cgcttgttgt tattctaatc gttttgctga ttgttttaaa gccattttct
      721 tccgggaaaa tctatcaggc gaatgatgtt attcccgaca atgcttttgc tgttttgcgc
      781 atcaacaatc cgggagaaat aattcataac agcagctata aaaatacact gagcaatatt
      841 tcattcatgc ctgcggccga agcttttgta tatggtttga tgcaggccga tactgtttta
      901 tcggttagcg aaagcagtaa aaatctgctt tcggaatcac aattgtatat ttcgtttcat
      961 cctgtcggaa aaaataaaac acagtgtttg tttgtgcttc agatgcctga aaaaacgaat
     1021 gcaaaactgt tgtacgaaga attgaataat agtggaaaag tggttttttc tgaaaaatca
     1081 attgatggga atatctgtta tacgattggt aaaaagttgt tcaatctttt tccatgcaat
     1141 aatttattga taggatcgct ttcgacgcaa ttgcttgaac aatccattgc agcatcggaa
     1201 aaaaaatcgg ggatttccaa taatgaagaa tttgtttcag ctcaaaaaac agcttcatct
     1261 gattgcgaaa ttaatatttt tgtaaacaca cccatgctct acaggtcgct tgggcagctt
     1321 gtttcgggaa acgcgtatgt aaatctcgcg tttttgcaaa cagtttcttc gtggggaaat
     1381 cttgatgtaa aattgcaaga tgatgtaatg gtgttttccg gattgtttcc actgagcaaa
     1441 gcaaatgcat cgtttatgtc gttgtttgat gagccagcca atggtgctac gcaagatgca
     1501 ttatcgatgt tgccggccaa tactgctgca gcattttcgt ttggattcgg ccattttgaa
     1561 aagttttttt ctgcgtatgc aaagagactt tctgaagata aacaaaacgt ttacaataat
     1621 gaagaagaaa tcgcacatct gaatcagcaa gcgaaaacag aatctatcga aaaatgtttt
     1681 tatcagcaca tcagtggtca gctttgcgtt ttttctacgt ccacaacagc tggtcgtgaa
     1741 cctcaggttt attgcgcttt tccggtaagc gacggggaag aagtgaaaga ttgtttgctt
     1801 gattatgctg cagatggaga gtgggatact gcgcaatttc gtaatgcaac actgatttgc
     1861 attccagtgg aatatggagc atttagtctc tttgggcctt tatttgagcc gatttcggag
     1921 acatgtattg ctgttacaga acattttgtt tttattggaa attctccgga acatgtaaag
     1981 ccggttctga atgatgtgct ttcggggcga acgttggggg cttctgaata taagcaaaac
     2041 ctgaccaatc atatcgcttc ttcgtggaat attttttcgt ttctgaatac cagcgcgtca
     2101 tcctataacc ttgaaactcg ctggctgagt gatacctcat taattgcatc cttgaatgat
     2161 tcgcataaaa acaaaaaagc cggtattatt tcaatgacgg ccgcacgtca gtcagatggt
     2221 attgtggtaa gtggtacctg gtttttcgga aagtcggatt cctcttcttc agcttcatgg
     2281 gcaacaacac ttgatgcaaa tatttcggga agacctgtga ttctgaagga ttttaaaacc
     2341 ggtgaattct ggattgcggt tgccgatgtg tatgataatg tttatttgct gaattcggat
     2401 ggtgtgatag tctggaaaaa agaagtgggt gatgcggtca atgacgattt ccagctgttg
     2461 tggtcgggca ataaggaaaa aagaagtatg gctttttcaa catcggatgg tatttatatt
     2521 attggccttg acggggaaat acggtcatca tttcctgttt ctgtcagagc gggtgttgca
     2581 tcaaacctgc ttgtggccga ttatgataat aatcagaaat atcgcttgat ttttggcgat
     2641 agtaaaggtg ttttaaataa ttatacaacg gatggaaaat caacggaggg ctggttgaaa
     2701 cctgatttaa aaaatcagca ccagtcggct gtgtttttca gtccgcttgg tggaagcgat
     2761 tatattgttg ttgcaagtcc cggaggtact gtacaggttt ttgaccgtaa gggaaaatcg
     2821 gtggttgata ttgatgaaat tcaggacaac aaaggatttt gtcctttgca gggtggacga
     2881 gatgtgaagt tctggactgc tttttcatcc gatggtgcac ttatcagcgt gaatcaccat
     2941 ggcgacatta atccgttaac ggaaaaatcc tatggagaga atgtgttgtt tgaagcaagt
     3001 aaaaatcagg ttcttgccat atctgccggc atattgtatg tttttaatgc tgaaggcagg
     3061 gaaacatcaa caataaaaat ttcggaatca gcggttgatt atatcaatgt gatttctgta
     3121 gaagacaacc attatttgat atttcataat tcagacaata tgattggtgt tgtttcgcag
     3181 gaatccggat ttaaggattt tttgtataaa tccgaggcgt ctgcttttgc ctgcattgtt
     3241 gttaatgaca agattctgat gatcacatcc aatgaaaaaa ccattgtgtt tgaaataatc
     3301 gcagcagaaa aggactgatg attttggaat ttgtcaggag tcgcgactct ttttttagct
     3361 cccgggactt catcccgggc taatctctgt cgcgccatcg gcgcttattg tacttcgcag
     3421 cttctatgac ggcttcgccg cttaacctca actttaacct tgaccttcga cctgattttt
     3481 tcctcttgac atctgccaat aaaatagcgt atatttggaa ctcttttttt gcaacgaaat
     3541 atgtttccat ttgtctaatt acaaaacctc ataccatgaa atttaaatca ctgtttgcgg
     3601 ctggatttct ggtctttgtg tccatgtttc tggcctcctg tgttaaggag gagtttgata
     3661 tggatactgt tactgcaact aattggaatc ccaatgttgc ggctccactt atcaacagca
     3721 gtttgtctct ttgggatgtg atgaatgatt atgattcaac ggacttgctg gtggttgatt
     3781 ccaatcagtt tgtgtatctg gtttacgaag acacggtata tagtgagtcg gccgaaaatc
     3841 tgatacctgt tgccaatcag acaatgaatt acagtgataa tcttactctg ggaacaatga
     3901 ccggggacta tgtttttgat tatgactata attttgattt tgctctgggc ggcggaatgg
     3961 agcttgattc aatggttttg aaagccggaa cactttccat ggcaatgaat tccgatttgt
     4021 catatccggc caccattgaa atgacgatac ctggttcaac actgaatggc gccccttttg
     4081 ccgttacgtt tgattatagc ggatccggct ccaatactac ggctccgttg agtggaggta
     4141 aatttgtttt tgacaattcc gtagttaata atcgtctggc agtgcatttt aaggtaacag
     4201 ttcatgccaa tggcagcgcc aacaatgcga cttatgccaa tttcaacatg aatcttgacg
     4261 gaatggcttt ccggtttttg tatggttatc ttggtcagtt caatctgagt ttgaataatg
     4321 ataccatcgg aatccgtgtt tataataata atttgacggg gactgtcaat tgggaagatc
     4381 cgcgtttata catcaatgtt tttaatggat ggggactgcc cattcgtaca acggtagatt
     4441 atatggaagc catccgctcc aacgatccac cggcctcggt gattatttcg ggtgccggaa
     4501 ttccgaatcc atgggatatt aattatccga cggcggttgg cagtgtttca acttcttctt
     4561 tctttctcga taaaaacaat tcaaatatcg atgcagcact caatatcacg ccgcaacgaa
     4621 ttctggcttt ggtttcgggt gtcaccaatc cgaatggcaa tgtggtgaaa aattttgtgg
     4681 aggatgttag cagaatggct gtggaagcca gactagaact gcctttttat ggtactgcgg
     4741 agggttttgt attgcaggat acttttgata tcagtttcgg cgaagacttg agtcatgttg
     4801 attggatcat gtttaaactc tatacaaaca atatgtttcc ggtagatgga acggttcaga
     4861 tttatttttg tgattcgctc aataaccggc tcgattcttt gctgaatcct gtcgatcagg
     4921 cattgctttc tgcgattccg ggaccagcgc ctgaatatga agtggtttca ccggtggcaa
     4981 aaactgtatc gcaaaccatt ggtggttctc gcatcgggaa tcttgaaaac acgaagaaaa
     5041 ttgttgtaac tgcctggctc gacacgtata atggcgggac tcagctggtg aaaatttatt
     5101 cgtattataa actcgatgta cgattgagcg cacaggcgca gcttaaattc aacagttccg
     5161 aaattgatta atgaaggagg ccaacatgaa aaaaacaatt attgctattt tcgtttttgc
     5221 atcagggctg ttgagtgtct ttccgttgat ggctcaaaat gatatgatta ttcagggaat
     5281 gcgtatcatt ccgcaatcat actattccaa tccggccttt attcctcaat gcagatggca
     5341 tatcggaatg ccggggcttt cttctttgta tatcggagga ggacacaatg gtttcaatgc
     5401 aaaaaatgtc atttccgtca attcgcttga ttctgttgaa ctcgatatcg aatcgatgat
     5461 cggcagtttg aaaaatcata attatgtatc tctcgaagca aacgaggaat tattgtcgtt
     5521 tggttttaaa atcaaagata agcactatat taattttgca gcaaccgaac gtgctttcgc
     5581 acgaatatca tatccgaagg acttgattga atttgctttc tacgggaatg gggccttgct
     5641 ggatcagaca ctcgatattg gaaatttccg gttgactgaa aatcattatc gcgaatttgc
     5701 ttttggttat tcctatgtgt atgataagaa ctggactttc ggtgcacgtg caaaagtttt
     5761 atttggtatg tccaatgtaa acacgcgtcg tactgatatt actttgcata ccgaaggtga
     5821 gtttttcgac attactgcaa cttcagatat tcttgtaaac accaacgaaa tcgacaatat
     5881 gtccgatacc gctatagatg aagaaaaata catgatggat tatctttttc ttacaaaaaa
     5941 caaaggtctt ggttttgata ttggagcgac ctataaactg gatgataaga ttacatttgg
     6001 acttagtgtg cttgatcttg gatttataag ctggaacagc aatccaatga atctttcgag
     6061 taaaaactct gatggcagtt ttacttttga cggggttgat gtttctgagt ttatggaaat
     6121 gggcgctgat tcttttatgc agaatcttgc cgacaccttg attgatattt ttgaagttga
     6181 tacaacttac gacaaatata aaacgtcgct caacactcgt attcatgcca gtgcctttta
     6241 taaggtaact gaaaaagatc atctttcagc attgcttcgt atgcatttct acgacagaaa
     6301 agtgcatcct tcattgagtc ttgcctatat gcgcaagttt ggtgatatct ggcatttgac
     6361 aggaacgtat tcaataacca accgcaatta tgttaatttt ggaatcggta tgtcgttgaa
     6421 attaggacca atgcaattgt atgtgttaac cgacaatatt ttcggaccgt taatctggaa
     6481 ccgttataca tggcttgaag aggaagaaga cgattttggt gcaacgtatt atgaaactga
     6541 aaaacttact attccgcgca actggaaata catgaatgtt catttcggat tgaattttgt
     6601 ctttggctgc aagccgccca aggacctcgt tccgattctt gaataattct tttgaattaa
     6661 aatttccgca tcatttaaat ccgtcttcat gaataactgg ggacggattt ttaaattttt
     6721 tgtgattggg gcttcacccc gggctaacat ctgtcgcgcc gttggcgctt tttgtgcttc
     6781 gctcttcttg tccctgggac tggcgcccga ggcaaaaata tattgcgcct tcagcgcatc
     6841 cgaaggtgct tcgcatcttt ctttgacgcc ttcggccact caccactcac cactcataac
     6901 tactaacacc tacttcctcg tccccgaagc cctaaagggc atacgggcta ttaatattta
     6961 act
//
LOCUS       DAOPKE010000127         6770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_11954_length_6770_cov_12.587342, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000127 DAOPKE010000000
VERSION     DAOPKE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_6770_cov_12.587342"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1213
                     /locus_tag="PKY63_11710"
     CDS             380..1213
                     /locus_tag="PKY63_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF01326.1"
                     /translation="MTEQFLTFNNKKLAYTIHGSGPAVMLIHGYTESKDIWFPMAEKL
                     GKKYMVIMPDLPGHGNSDVMENLTMEIMAKQMDVLADKLQLERFTMIGHSMGGYMTAC
                     YASLNERRLNGFGFFHSSARGDTAEGKSNRDRVMEIIEKDHGDTFINMFVPSLFYEAN
                     RDRLESEIKTLQQRALKISKKALIECQKAMAARMGHIDLIADSNLPILYIIGKQDSRS
                     DYNLILAQAALAKRAFVLSLDSCGHMGYLEKFDDCLAAVEGFLMACLQTNDSMTAIGG
                     R"
     gene            complement(1233..1616)
                     /locus_tag="PKY63_11715"
     CDS             complement(1233..1616)
                     /locus_tag="PKY63_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF01327.1"
                     /translation="MLRHIGIHIHDEKDVKDFYHEILGFQELNRFDLSEKTAHNVFGT
                     QHHIEVVRMKQFELMIELMICPEEKSDGMSHMALEYWKAESVAEKAKEKGYTVIEFQK
                     DNDHTGRYIKDKAGNIFEIKDINFG"
     gene            complement(1676..3811)
                     /locus_tag="PKY63_11720"
     CDS             complement(1676..3811)
                     /locus_tag="PKY63_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPF01328.1"
                     /translation="MKVYQTGDIRNIALVGGAKSGKTTLAECLLLEGGVINRRGTVED
                     KNTVSDYREIELERQNSVFSTVLYAEYSGKKINIIDTPGFDDFVGDVLAALTVSDLAV
                     MLINAQNGIEVTTEISWRNLTRMNKPVMFVMNQLDHEKANFDEVLRELQSQFGNKVIP
                     VQYPVSTGVGFNSVVDVLTKKLYKYPANGGKPEVLDIPESEKDKADAMRTALIEAAAE
                     SDEALMEKFFENGDLSEEELVNGLAASIIQRNVFPLLCTSSKHNIGAGRIMEFVAQFG
                     PSPLQSVPMKSKSGKEYHYKAADPFCGFVFKTSIEQHLGEISFFKIAAGEISEGSDVI
                     NGTNSTKERVTQIFAVAGKNREKLEKAVAGDIVATVKLKNTATNETLNNPKNSDEEIV
                     SIQYPDPKYRTAIKAQNAADDEKLGAVLNELKKIDPTYLVEYSKELKQIIVSCQGEMH
                     MNAAKWHMETIEKVMIELYPPKIPYRETITKAAKAMYRHKKQSGGAGQFGEVHMMIEP
                     WREGMSWTTEFPVRGSDEHPLPWGGKLVFNNCIVGGAIDARFMPAILKGIMEKIEEGP
                     LTGSYARDIVVYVYDGKMHPVDSNELSFKLAGRQAFREAFKNAGPKILEPIYDVEVMV
                     PADRMGDVMTDLQGRRAVVMGMDSEGNYQKIKARVPLAEMNRYSTALSSLTSGKATYT
                     MKMAEYQQVPTDVQTALLKAYEEQEKDEE"
     gene            complement(3950..4498)
                     /locus_tag="PKY63_11725"
     CDS             complement(3950..4498)
                     /locus_tag="PKY63_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HPF01329.1"
                     /translation="MKTRYIALLRGINVGGKNVIPMKDLKALFEQCGFCDIETYIQSG
                     NVMFSCGVSDSSKIRKKIEINLKKYSGADIPVVLLTAEQLSFVVANAPEWFGAEPEKY
                     RYDVVFLDSEIEAEALFSKISLREGVDRAAAGNGVLYFSKLIEKATQSRLSKIVSLPE
                     YRLMTVRNWNTTRKILELVHSK"
     gene            complement(4749..5606)
                     /locus_tag="PKY63_11730"
     CDS             complement(4749..5606)
                     /locus_tag="PKY63_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01330.1"
                     /translation="MKNVIVYSIIALLSIQLMAQNPDKAHDLVSQGVQLHDKGDYNAA
                     IVKYDEALRADKDNLMAYSEKAYSLSALKKYEDCITCCKKAITAHPNDSGLNMIYVSY
                     GSALDHLGEPDKAVAMYDEGLTLLPDFYLLHYNKGITLIGQDKSDEALLSFQKAVVCN
                     PEHASSHNAIARVMHYKNKRIPALLAYCRFFTVEPTGPRAEDNLNRTKDILSLGVKKT
                     GKNSISISIDGSSLGDTTADGKPNENSFSTVDLIMSMSAALAISENKKQTDAEKFAGN
                     LNTAIASME"
     gene            5733..6629
                     /locus_tag="PKY63_11735"
     CDS             5733..6629
                     /locus_tag="PKY63_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HPF01331.1"
                     /translation="MKFSGQNILGFMDQFPDEITCVAYLADVKWADKFVCSKCGHDKF
                     TVRKKNLARDCNRCHHVESATAGTLFHRVRFGLRKAFAIVFEMSTTTKSLSSSIVAKR
                     YSISRTTAWTFMHKVRIAMKSSMANPLSGTVQVDEFVFGGMETLKQGRSHDAKRKKIV
                     AAIETSTTGGVKRVYFQKIPDFSSKSLRKIFDDHISAQAKVNTDHWTGYTPIAKDFNI
                     DQKYSDNGKGMKQMHIIIHQVKAWLRCTFSWVHEGHIEKYLDEFSFRINRSIHRQNIF
                     HTLIQRMILAKPSPYQLIKIST"
BASE COUNT         1795 a         1433 c         1502 g         2040 t
ORIGIN      
        1 tgtaaagttg aaaggtgacg taaatgcttg tagcatgcgg cgcgcaatgc atagtgcatg
       61 gagtaaactt cgctctcgca ctgaagtcac tctgagcgga gtcgaagagt cattttgtat
      121 ttgacgtgcg gcactatgcg taaagcaaaa tcgccacagc tccttcttgg agcaccgacg
      181 cttcggccgg cacatattgc tgaaagctta ttatcccgtg ccttgcggac gggacttcgc
      241 tgattgatga atgctgcttg tccttgacct catcgttaat cttgaacttc gtacttttgt
      301 aacgggtaac gcttgtaagg cgtaacgact gtaacgtccg taacgattat tttcattact
      361 ttgaataaac gctttcaaca tgacagaaca atttctgacc ttcaacaata aaaaactggc
      421 atacaccatt catggctccg gacctgctgt gatgcttatt cacggatata ctgaatcaaa
      481 ggatatctgg tttccgatgg cagagaaact gggaaaaaaa tatatggtaa tcatgcccga
      541 tttgcctggc catggcaata gcgatgtgat ggaaaatctt acaatggaaa tcatggcaaa
      601 gcaaatggat gtgttggccg ataagcttca actggagcgg tttaccatga ttggtcacag
      661 tatgggtgga tatatgaccg cctgttatgc ttcactgaat gagcgtcgtt tgaatgggtt
      721 tggttttttt cattccagtg cccggggcga cactgcagaa ggaaaatcca acagagaccg
      781 ggtgatggaa attattgaaa aagatcatgg ggatactttt atcaatatgt ttgttccttc
      841 tttattttat gaagcaaatc gcgatagact tgagtccgag attaaaactt tgcaacaacg
      901 tgcattaaaa ataagtaaaa aggcgctgat tgaatgtcag aaagccatgg ctgcccgtat
      961 gggacatatt gatctgattg ctgattccaa tcttccaatt ttatatatta tcgggaagca
     1021 ggacagtcgg tccgattaca atcttatatt agctcaggca gcgcttgcaa agcgggcttt
     1081 tgtattgtcg ctggatagtt gtgggcatat gggctatctc gaaaaattcg atgattgtct
     1141 tgctgccgtt gaaggttttt tgatggcgtg tttacagacc aacgattcca tgaccgccat
     1201 aggcggacga tgaaatgttg gtcaatatct gatcaaccga aattaatgtc tttgatttcg
     1261 aaaatatttc ccgctttgtc tttgatgtag cggcctgtat ggtcgttgtc tttctgaaat
     1321 tcaataacag tgtatccttt ttcttttgct ttttcggcaa cactttctgc tttccagtat
     1381 tcaagcgcca tgtggctcat gccatccgat ttttcttcgg ggcaaatcat cagctcaatc
     1441 atcagctcga attgtttcat gcgaacaact tcaatgtgat gttgagtgcc aaaaacattg
     1501 tgagctgttt tttcgctcaa atcgaaacgg ttcaactcct gaaatcccag aatttcgtga
     1561 taaaaatcct tgacgtcttt ttcgtcatga atatgaattc cgatatgacg caacatgcga
     1621 ttgttattta gaaaaacacc ctgtcgtgac agggtgtttt tggtatttat aaaaactatt
     1681 cttcgtcttt ttcctgttct tcgtatgctt tgagcagggc tgtctgaaca tcggtcggta
     1741 cctgttggta ttcggccatt ttcatggtgt aagtggcttt acccgaagtg agtgaactca
     1801 acgctgttga atagcggttc atttcggcca gcggaacgcg tgctttgatc ttctggtagt
     1861 ttccttcact gtccatcccc attacaacgg cgcgacgtcc ctgaaggtca gtcattacat
     1921 cacccatgcg gtcggcggga accatgactt caacatcata aatcggctcg agaattttcg
     1981 gaccggcatt tttgaaagct tcacggaaag cctgacgacc agccagtttg aatgataatt
     2041 cgttggagtc aaccgggtgc attttgccgt catacacata aacaacaatg tcgcgtgcat
     2101 atgaaccggt gagcggacct tcttcgattt tttccatgat accttttaga atggccggca
     2161 tgaaacgtgc atcgatggct ccaccaacga tacagttatt gaaaaccagc tttccacccc
     2221 atggcagcgg atgttcatcg cttccacgta cagggaattc tgtggtccag ctcatgcctt
     2281 cgcgccacgg ttcaatcatc atgtggactt cgccgaactg gccggcacca cctgattgtt
     2341 ttttatgacg atacatggct ttagctgctt tggtgatggt ttcgcgataa ggaatctttg
     2401 gtggatagag ctcaatcatc actttctcga tggtttccat gtgccatttg gctgcattca
     2461 tgtgcatttc gccctggcag ctgacaataa tctgtttgag ttcctttgag tattcaacga
     2521 ggtatgtcgg gtcgattttt ttcagttcgt tcagaacagc accgagtttt tcatcatcgg
     2581 cagcgttttg tgctttgatg gcagtgcggt atttgggatc cggatactga atcgaaacaa
     2641 tctcttcatc agaatttttc ggattgttca gtgtttcgtt ggttgccgta tttttcagtt
     2701 taacagtagc cacgatatct ccggccacag ctttttcgag tttctcgcgg tttttaccgg
     2761 caacggcaaa aatctgagta acacgttcct tagtgctgtt ggttccgttg ataacatccg
     2821 aaccttctga aatctcaccg gcagcgattt tgaagaaact gatttcgcca aggtgctgtt
     2881 cgatgctggt tttgaaaacg aatccgcaga aaggatcggc agctttgtaa tgatattctt
     2941 ttccgctttt tgatttcatc ggaacgctct ggagcggtga aggaccaaac tgggcaacga
     3001 attccattat gcggccagcg cctatgttgt gtttagagct ggtgcagagg agtgggaaaa
     3061 cattgcgctg aatgatactg gcggcaagtc cattgacgag ttcttcctcg ctcaggtcac
     3121 cgttttcgaa gaatttttcc atcagagctt cgtctgattc agcggcggct tcaatcaatg
     3181 ccgtgcgcat ggcgtcggct ttgtcttttt cactttcggg aatatccagt acttcgggtt
     3241 ttcctccgtt agcaggatat ttatacagtt ttttggtcag cacatcaacc acagagttga
     3301 atccgacgcc agttgaaact ggatactgaa ccggaatcac cttgttgccg aactggctct
     3361 ggagctcgcg gaggacttcg tcgaagtttg ctttttcgtg atcaagctgg ttcatgacga
     3421 acatcactgg cttgttcatt cgggtgaggt tgcgccagct gatttcagtg gtcacttcaa
     3481 tgccgttctg cgcgttgatg agcatcacgg cgaggtcgct cacggtgaga gctgccaaca
     3541 cgtcgcccac gaagtcgtcg aaacccggag tgtcgattat gttgattttc tttccgctgt
     3601 attcggcata caacacggtt gagaaaacag aattctgcct ttcgagctca atttcacggt
     3661 aatccgaaac agtgtttttg tcttcaacag tgccgcggcg gttaataaca ccaccttcaa
     3721 gcagtaaaca ctcggccaga gtcgtttttc cagatttggc accgccaacc agcgcgatgt
     3781 tgcggatatc tcctgtctga tacactttca tatgttgttc tgatttaaat ttttaaagag
     3841 ttactccaaa aaggagcaca aaaatatgaa tttattcgga ttggcaacac cggaatgcgt
     3901 aaaaatgaag aaattaagag gaaattgccg gttttaattc cggatattat catttggaat
     3961 gaacgagctc aagaattttt cgtgttgtat tccagtttcg caccgtcatc agtcggtatt
     4021 cgggcaacga aactattttt gaaagccggc tctgagtggc cttttcaatt agttttgaga
     4081 aatataaaac accgttgccg gctgcagcac gatcgacccc ttcgcgaaga gaaattttcg
     4141 aaaacaatgc ttcagcctca atttcagaat ccagaaaaac cacatcgtat ctgtactttt
     4201 ccggttcagc cccgaaccat tccggagcgt ttgcaaccac gaagctgagt tgttcggcag
     4261 ttagcaaaac aaccggaatg tcggcacccg aatatttttt caggttgatt tcaattttct
     4321 ttctgatttt tgaggaatcg gaaacaccgc atgaaaacat cacattaccg ctctggatat
     4381 atgtttcaat atcacaaaat ccacattgtt caaacaatgc tttcagatct ttcatcggaa
     4441 tcacattctt tccgccgaca ttgatgccgc ggaggagagc gatgtagcgg gttttcatac
     4501 ggtcggcaaa tcagcgcaaa gcgcattatg ttgtagcaga attattccga ataccatttg
     4561 tatgattcgg accaggccag aaagtcctga atttctctgg cgtgcgattt aatccatttg
     4621 gtcacatgtt tttcaccgga ggaggcgaac accaaatagg acaatgtctc aatatatttc
     4681 cggttcttca tttcgatgaa ataggggaca taataatccc agtaaaaacc gtaattatcg
     4741 gcttttgttt actccatgga tgctatagca gtatttaagt tgccggcaaa tttttcagcg
     4801 tcggtttgct ttttattttc actgatggca agtgcagccg acattgacat aataagatca
     4861 acggttgaaa agctgttttc gtttggtttg ccatctgccg ttgtatcgcc aagagaactg
     4921 ccatcgatac tgatggaaat ggaatttttt cctgtttttt ttacgcctaa actcagaatg
     4981 tcttttgtcc tgttcagatt atcttcagcc cggggaccag taggctcgac ggtgaagaag
     5041 cggcaataag ccagcaatgc aggaatgcgt ttgtttttat aatgcatcac gcgtgcaatg
     5101 gcgttgtgag aacttgcgtg ttcggggtta catacaacag ctttctgaaa actcaacagt
     5161 gcctcatcgc ttttatcctg tccgatcagc gtgataccct tgttgtaatg aagcagatag
     5221 aaatcaggca acagcgtcaa tccttcatca tacatggcaa ctgctttatc cggctctccc
     5281 agatgatcca gtgcgctgcc ataacttaca tagatcatat taagtcctga gtcattggga
     5341 tgcgcagtta tggccttctt gcagcaggta atgcaatctt cgtatttttt gagtgcactg
     5401 agtgaatacg ctttttcgga atatgccatc agattgtcct tgtcggctct cagtgcttcg
     5461 tcatacttaa caatggcggc attataatct cctttatcat gtaactgaac tccctgcgat
     5521 accagatcgt gtgctttgtc ggggttctgg gccatcagct gaatgctcag caaggcaatt
     5581 atagaataaa cgataacatt tttcatgatg acaaaaaatt tggttggtaa gcaaatatat
     5641 aaatatttgt ttacagctgg taaaaattta attaaggtgt ccaaataatt tcttatatta
     5701 tggtcgttta aaatgtctaa atattaatca atatgaaatt ttccggacag aatattttag
     5761 gattcatgga tcagtttcct gatgaaatca catgtgtagc atatctggca gatgtgaaat
     5821 gggctgacaa atttgtttgt tcaaaatgcg gacacgacaa attcacagta agaaagaaga
     5881 atcttgcaag agactgcaac aggtgtcacc atgttgaaag tgcgacagcc ggtacacttt
     5941 ttcacagggt tcggtttggc ctcagaaaag cgtttgcaat tgtttttgaa atgtcaacca
     6001 ccaccaaaag cctttcatca tcaatagttg caaagcgcta cagcataagc agaacaactg
     6061 cgtggacatt tatgcataag gttagaattg caatgaaaag cagcatggcc aatccacttt
     6121 caggaactgt gcaggttgat gaatttgttt ttggtggcat ggaaactcta aagcagggca
     6181 gaagccatga tgcgaagcgg aaaaagattg ttgcagccat tgaaacatca actaccggag
     6241 gagtgaaacg cgtatatttt cagaaaatcc ctgatttttc ctcaaaatca cttcgtaaaa
     6301 tttttgacga tcatatttct gcgcaagcca aagtgaatac tgaccactgg acaggctaca
     6361 cgccaattgc gaaagatttc aatattgatc agaaatactc ggataatggg aaaggtatga
     6421 agcaaatgca catcattatt catcaggtga aggcctggtt aaggtgcact ttttcctggg
     6481 tacatgaagg gcatattgaa aaatatctgg atgaattcag tttcagaata aatcgctcca
     6541 ttcacaggca aaacattttt cacaccctga ttcaaaggat gattctggca aaaccatctc
     6601 cttatcagtt gattaaaata agtacttaat tggacacctc aaaaatttaa ttttatttcg
     6661 tagtgaaaaa acgcgggtat atattaaaag tcggcctgac cggatttttt caacatcatc
     6721 tctccatcaa aaacaccata tgttttgtgg ttgacccatt cgcccaggtt
//
LOCUS       DAOPKE010000128         6734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12038_length_6734_cov_13.288217, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000128 DAOPKE010000000
VERSION     DAOPKE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12038_length_6734_cov_13.288217"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKY63_11740"
     CDS             complement(<1..251)
                     /locus_tag="PKY63_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HPF01332.1"
                     /translation="MYKVDITTINNLSQKAKASDRRRMNLNIHKTEEDVMQRFLNAME
                     PGTYIRPHKHEMPHKRELFAVVRGEFLMLEFDDTGNVTDS"
     gene            337..984
                     /locus_tag="PKY63_11745"
     CDS             337..984
                     /locus_tag="PKY63_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01333.1"
                     /translation="MDEIEFRLLKRIAAGESQTLDFKFAVNDSRKIARSMSAFANTDG
                     GSLLIGVKDNGRLAGMRTEEEIYMAEAAASMFCVPEILFNSILYKIQGKEILEIVIPA
                     LSDVLTLAPDENEKYLAYLRFRDANFVAGSIYERVWNKRRFKLNTHLRMDEYHRAVLE
                     LMERKGAVSLEQVVHDLGLKKGFAASVIERLILLDAADFCLNEKGMHYCLKDSIQ"
     gene            1124..1378
                     /locus_tag="PKY63_11750"
     CDS             1124..1378
                     /locus_tag="PKY63_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01334.1"
                     /translation="MLTTILLVILGLYLFSQIMVRWVMPWLLRRYIMRMQERMNPDAG
                     QKTSRKDATEVHIPRKQKSKSSRTYNNAEYVDYEDVDDGK"
     gene            complement(1882..2559)
                     /gene="lipB"
                     /locus_tag="PKY63_11755"
     CDS             complement(1882..2559)
                     /gene="lipB"
                     /locus_tag="PKY63_11755"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPF01335.1"
                     /translation="MQIDYTDIGHMEYQAAWDFQQARMDELIEMRKNPDYSSNPFAGW
                     FLAVEHPHVYTLGKSGDEQNLLLNYIQLQAKNAQFIHTNRGGDITYHGPGQMVGYPII
                     DLDKLNLSVRRYIYLMEEAIILALAEFGIKTERLEGATGVWIDSLTPGKARKICAIGV
                     RVSRFVTMHGFALNINSDLSYFKHINPCGFTDKDVTSMEKELGAPQDMEKVKRLVYEK
                     FVRLIQA"
     gene            complement(2593..2665)
                     /locus_tag="PKY63_11760"
     tRNA            complement(2593..2665)
                     /locus_tag="PKY63_11760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Thr,seq:cgt)
     gene            complement(2675..2747)
                     /locus_tag="PKY63_11765"
     tRNA            complement(2675..2747)
                     /locus_tag="PKY63_11765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2712..2714),aa:Pro,seq:cgg)
     gene            3158..3796
                     /locus_tag="PKY63_11770"
     CDS             3158..3796
                     /locus_tag="PKY63_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPF01336.1"
                     /translation="MIFVNSISFHTFIRIFCMEKVELEISGISYSYSQSGAYTLILSE
                     SGGRRKLPIIIGQFEAQSIAIEMEKMKPTRPLTHDLFRSLAQSFNIKLNEVFIHKFEE
                     GVFHSMLIFEKENQIHSLDSRTSDAVALAIRFNCPIYTSEEILNEVGVIFDEQAQDSI
                     EEESEEKDTPVSSSSGLASLTLDELEAMLTEAIDNEDYEFASQIRDEINRRK"
     gene            3815..5077
                     /locus_tag="PKY63_11775"
     CDS             3815..5077
                     /locus_tag="PKY63_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPF01337.1"
                     /translation="MGLKLRLTVMNFLQFYIWGSWLITIGNFWFGTKQWSGAEFGIIF
                     STMGIASLFMPALMGIVADRWLNAEKLYGILHLLSGAALFYLPQIQDPTGFFWGILVA
                     MTFYMPTIALTNSVSYTALKNNGFDVVKAFPPIRVFGTIGFIAAMWVVNLTGNKASEN
                     QFYIAAAISVLLGLYSFTLPKCPPLGKKQENRTFIQMLGLDAFELFKSTKMALFFIFS
                     MFLGAALQLTNAYGDVFLGEFKKIPEYADSFVVRYSTIIMSISQMSETLFILAIPFFM
                     KKFGIKKVMLISMVAWVLRFALFGLGNPTDGLWMIILSCIIYGMAFDFFNISGSLFVE
                     TTTKPAIRSSAQGLFMMMTNGFGAIMGSTISGYVIQQYYTDANGVRDWRNIWLLFAAY
                     ALVVAILFAIFFRHKHNPDDPKLQNIAH"
     gene            5098..5940
                     /locus_tag="PKY63_11780"
     CDS             5098..5940
                     /locus_tag="PKY63_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPF01338.1"
                     /translation="MKLKKIRQKTQKAITVVFFLLFGFYFSLIVVAQTGTKFSGVASY
                     YGIPFHGRQTASGEIFSMDSMTAAHKQLAFGTLVRVTNRSNGKSCVVKINDRGPFIAG
                     RMIDLSKAAADTLDFIAQGLVTVDCEIIGTATINRPVLASTSVKNDKAKPDKGAAKEV
                     RLQDSTATKTAAANTTRYIPREAVKYVAASPDSLTFYSVQLGSFGMQQNAEALLKKVE
                     GKFPDSRMMQAEVYGKIWFRVLCGKYPTIDEATKLKNSLIKTYPQSFLVKFEHAEQNT
                     ETTE"
     gene            5940..6734
                     /locus_tag="PKY63_11785"
     CDS             5940..6734
                     /locus_tag="PKY63_11785"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPF01339.1"
                     /translation="MKQYHDLMRHVLDNGVKKPDRTGTGTVSVFGYQMRFNLQDGFPL
                     LTTKKLHTKSILHELLWFLKGDTNIQYLKENGVSIWDEWADENGNLGPVYGSQWRSWK
                     GSEGQTIDQISELIDQIKKTPDSRRLIVSAWNVTDVPKMKLPPCHLLFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYAFLLHMVAQATDLQVGDFVHTFGDAHLYLNHIEQAQL
                     QLSRDCRPLPKLEINPEVKNIFDFKYEDFSITGYDPHPHIKAVVAV"
BASE COUNT         1878 a         1393 c         1531 g         1932 t
ORIGIN      
        1 gaatctgtca cgtttcctgt atcatcgaat tcaagcatca gaaactctcc gcgaacaacg
       61 gcgaaaagtt cgcgtttgtg aggcatctca tgtttgtgtg gtcgaatgta ggtgccgggt
      121 tccatggcgt tcaaaaaacg ctgcatgaca tcttcttcgg ttttatggat attcagattc
      181 atgcgtctgc gatccgatgc tttggctttt tgcgacagat tattgatggt tgtgatatcg
      241 actttgtaca taactttaat tttgaatgga taaaggtaca aaaaaaattg aaataataaa
      301 cagaaccatt aaatttgtat cgttgtgcat atatgtatgg atgaaattga atttcgttta
      361 ttgaagcgga ttgcagctgg tgaaagccaa acgcttgatt ttaaatttgc ggttaacgac
      421 agccgcaaga ttgcccggag catgtcggct tttgccaata ccgatggggg gtcattgctt
      481 attggtgtaa aggacaatgg ccgccttgcc ggaatgcgta ccgaagagga gatttatatg
      541 gccgaggcgg ctgccagtat gttttgtgtc cctgaaattt tattcaatag tattttatat
      601 aaaatacaag gtaaggaaat tctcgaaatt gttatccccg ctttgtccga cgtgcttact
      661 ctggctccgg atgaaaatga aaagtatctg gcttatcttc ggtttcgcga tgcaaatttt
      721 gtagccggca gtatttatga gcgtgtctgg aataaacgac gttttaaact gaatacgcac
      781 ttgagaatgg atgaatatca cagggctgtg cttgagctta tggaacgcaa aggcgctgtt
      841 tctttagaac aggttgttca tgatctcggc ttgaaaaaag gttttgctgc ttctgtcatt
      901 gaacgactca ttttgcttga tgccgctgat ttttgtctca atgaaaaagg aatgcattat
      961 tgtctgaaag atagtattca gtagtcagtg gccagtagtc agtatgattt actcacaact
     1021 gactactcac aactgactac tgattttctt ccattcaccg gtcgcagccc tcaattcacc
     1081 gttaaccatt aattgataac ggctaatttt gtacttttgc accatgctta caacaattct
     1141 tctggtcata ttaggattgt atctgttttc gcagataatg gtgcgctggg tcatgccttg
     1201 gttgttgcgc agatatatta tgcgcatgca ggaacgcatg aatcccgatg ccgggcaaaa
     1261 aacatcacga aaagacgcaa cggaagttca tatcccacga aagcaaaaat cgaaatcaag
     1321 ccgtacctac aacaatgccg agtacgttga ttacgaagat gtggacgacg ggaaataaat
     1381 tggtttttcg gttctaaaag gtcatgtggt gtattaatta ttaccgaatg ttaaaaatga
     1441 gctttttacc atgagcgtga catccggagg atgtcgaata ataatagcgt ccggcgacct
     1501 tgtcatcacg tggaacgtga caagctgttt cacgtgaagc cggatgtaga aaggcacaaa
     1561 aacaggatcc gccagctggc ggatccaatc caccttcatt atatttttct tattgcaata
     1621 aatttgtatt gatttgacct ctctgaggtc ggtttctaaa tcatttttac ccggccattt
     1681 cacgtaaaac gtgacacagc aggccgggct attgttgttt gacctgcgct gcaggtcggg
     1741 caaaatattc agacttcgtg gttcacacaa aaacatgtag aaccataaaa acaaattatg
     1801 tatcacatat tttgtgtgac acattttgcg tcatgaaaaa taatttgcga aaaaattctt
     1861 gcgatggctt tgagttaatg atcaggcctg aatcagcctg acaaacttct catagaccag
     1921 ccgtttcacc ttttccatat cctgcggggc gccgagttct ttttccatcg aggtcacatc
     1981 tttgtcggta aagccgcatg gattgatgtg tttgaaatag gacaggtctg aattgatgtt
     2041 gagtgcaaag ccatgcatgg tcacaaagcg gcttacacga acaccgatgg cgcatatttt
     2101 acgcgctttg cctggggtga gcgaatcaat ccacacgccg gtggcgcctt cgagtcgctc
     2161 tgttttgatg ccaaattcag ccaatgcaag tatgattgct tcttccatta aataaatgta
     2221 gcggcgaacc gaaagattca gcttgtcgag atctatgatc ggatagccca ccatctggcc
     2281 gggaccgtga taggtgatgt cgccgccgcg attggtgtgg atgaattgag catttttggc
     2341 ctgaagctga atgtagttga gcagcagatt ctgctcgtcg ccgctcttgc caagggtata
     2401 tacatgcgga tgctcaacag cgagaaacca acccgcaaaa gggtttgatg aataatccgg
     2461 atttttccgc atttcaatca gttcatccat gcgggcttgc tgaaagtccc atgccgcctg
     2521 atattccatg tgcccaatgt cggtgtagtc gatttgcata aaatgaaaaa agctgcctgt
     2581 gcaacttcat tgagccacta atgggactcg aacccacgac ctgctcatta cgaatgagct
     2641 gctctaccaa ctgagctaaa gtggcgagaa aaagtcggga tgactggact cgaaccagcg
     2701 gcctcttcgt cccgaacgaa gcacgctacc aactgcgcta catcccgatg ggggtgcaaa
     2761 ggtacaatat tttttgaata atgaacatgg aataatgaac caggaagtaa gaagttaatt
     2821 gcaaggatgt tatacttttt cgttacgcgt tacatacttt acaatcgtta cagggtgaca
     2881 aggtgaggaa tagccttcag taatcagctc tgagaaggaa ctgggtgttt tatgtggcga
     2941 tgatgcgata cggcgatgat gcgacaaatc gaatttcgca taattgcaaa atcgcattaa
     3001 cgccaaacgc agtgatagaa cgaaaccaag ccatgctcta tgcctcttgc gctatgcgtt
     3061 gtgtattgcg cgttgtgccg taagcccttt gccctatgcc gcatgcctca tgccggttta
     3121 ttaaagctaa caaaggccgg ttaacaaact gctgcaaatg atatttgtta attcaataag
     3181 ttttcatact tttatccgga ttttctgtat ggaaaaagtt gaactcgaaa tcagcggcat
     3241 cagttacagc tactcacaga gtggtgccta cacgcttata ctgtccgaat cgggcggcag
     3301 gcgtaagttg cccattatta tcgggcagtt cgaagcgcag agcattgcta ttgaaatgga
     3361 gaagatgaaa cccacacgtc cgcttacgca cgatctgttt cgtagtctgg cacagtcttt
     3421 caatattaaa ctcaatgagg tctttatcca taaattcgaa gaaggcgttt ttcacagcat
     3481 gcttattttt gagaaagaaa atcagattca ttctctcgat tcgcgcacaa gtgatgctgt
     3541 tgcactcgca attcgtttca attgcccgat ttatacaagt gaagaaattc tgaacgaagt
     3601 cggtgtcatt tttgatgaac aggcacagga ttcgattgaa gaggaatctg aagaaaagga
     3661 cactcctgta agttcttcat caggccttgc ttcccttaca ctcgatgagc tcgaagccat
     3721 gctgaccgaa gccatcgata acgaggatta tgagtttgca tcgcaaatcc gcgatgaaat
     3781 caaccgtaga aaataatcac aaaatacttc tgttatggga ctgaaactta gactaacagt
     3841 catgaatttt ctccagtttt acatctgggg atcgtggctc attaccatcg gcaacttttg
     3901 gtttggcacc aaacaatggt ccggtgctga atttggaatt attttttcaa ccatgggcat
     3961 cgcatctttg tttatgccgg ctttaatggg cattgtggcc gaccgctggc tgaatgctga
     4021 aaaattgtat ggaattctgc atttgttgag tggtgccgct ttgttttatc tgccacagat
     4081 tcaggatccg accggatttt tctggggaat tctggtcgcc atgacatttt acatgccaac
     4141 cattgcactc accaactctg tttcatacac agcgcttaaa aacaatggat tcgatgtagt
     4201 gaaagctttt ccacctattc gggtttttgg taccattggt ttcattgcag ctatgtgggt
     4261 ggtaaacctc acaggcaata aggcctcgga aaatcaattt tatattgccg cagccatttc
     4321 tgttttactt ggactgtatt cattcacact gcctaaatgt ccgccgcttg gcaaaaaaca
     4381 agaaaacaga acttttattc agatgctcgg cctcgatgca tttgagctgt tcaaatctac
     4441 caaaatggcg ttgttcttca tcttttcaat gttcctggga gctgctctgc aattaacaaa
     4501 tgcttacggc gatgtttttc tgggcgagtt taaaaaaatt ccggaatacg ccgattcatt
     4561 tgttgtgcgc tattcaacaa ttattatgtc catttcccag atgtccgaaa cactctttat
     4621 tctggccatt ccgttcttta tgaaaaaatt cggaatcaag aaagtgatgc tcatcagtat
     4681 ggttgcatgg gtgcttcgtt ttgctttgtt cggtcttggt aatccaaccg atggtttgtg
     4741 gatgattatt ctgagctgca ttatttatgg aatggctttc gattttttca atatttcagg
     4801 atcgcttttt gttgaaacaa ccaccaagcc tgcaattcgt tctagcgcac aaggattgtt
     4861 catgatgatg accaacggat ttggagccat catgggcagc acaataagcg gatatgtcat
     4921 tcagcaatac tataccgatg ccaacggagt gcgcgactgg agaaatatct ggttgctttt
     4981 tgctgcatat gctcttgttg tagccatttt atttgcaata ttcttcaggc ataagcataa
     5041 tcccgatgac ccaaaattgc agaatatcgc tcattaaaat ttaacggaga gtaggttatg
     5101 aaactaaaaa aaatacgaca gaaaacgcaa aaggcaatca ctgtcgtgtt ttttcttttg
     5161 ttcggatttt acttttcgtt aatcgttgta gcccagacag gaacgaaatt ttcaggagtt
     5221 gcttcttatt atggtatccc ctttcacgga aggcaaactg ctagcggaga aatattttca
     5281 atggattcga tgaccgcagc acacaagcaa ttggcttttg gaacattggt cagggtgacc
     5341 aatcgcagca atggaaaatc atgcgtggtg aaaatcaatg accgcgggcc tttcattgca
     5401 ggtcgcatga ttgatctgag taaggcagct gccgatacgc ttgatttcat tgcgcaggga
     5461 cttgttaccg ttgattgcga aattattgga acggcaacga tcaacaggcc tgtactggct
     5521 tccacttcag taaaaaacga taaagctaaa ccagataaag gagctgccaa agaagtcaga
     5581 ttgcaggata gcactgctac gaaaacggca gcagcaaata caacgaggta cattccgcgc
     5641 gaagctgtga aatacgtggc tgcaagtccc gattcgctta cattctattc agttcagctt
     5701 ggaagttttg gcatgcagca aaatgcagaa gcgttgttga aaaaagtgga agggaaattt
     5761 ccggattcac gaatgatgca ggccgaagtg tatggaaaaa tctggttccg tgtactttgc
     5821 ggcaaatacc cgaccattga tgaagctaca aaattgaaaa actcgttaat caaaacgtac
     5881 ccgcagagtt ttttggttaa atttgaacac gcggaacaaa atacagaaac aacggagtaa
     5941 tgaaacaata ccatgattta atgcggcatg tgcttgataa cggtgtgaaa aaacccgacc
     6001 gcaccggaac cggcactgtg agcgtatttg gatatcagat gcggttcaat ctgcaggacg
     6061 gatttccgct gttgactaca aaaaaactgc acaccaaaag tatattgcac gaattgttgt
     6121 ggttcctgaa aggcgataca aacattcagt atctgaaaga aaacggtgta tcgatttggg
     6181 atgaatgggc cgacgaaaac ggaaatcttg gacctgtata tggctcgcaa tggcgcagct
     6241 ggaaaggcag cgaaggacaa accatcgatc agatttcgga gttgattgat cagattaaaa
     6301 agacccccga ttcacgcaga ttgattgtga gtgcatggaa tgtgaccgat gtcccgaaaa
     6361 tgaaattgcc accgtgtcat ttgctttttc agttttatgt ggccaatgga aaactgtcgt
     6421 gtcagctcta tcagcgcagt gctgatattt ttcttggtgt cccctttaat atcgcttcct
     6481 atgctttttt gctgcacatg gtggcacagg caactgatct gcaggtaggc gattttgttc
     6541 acaccttcgg cgatgcacat ttgtatctga atcacatcga gcaggcacaa ctgcagctaa
     6601 gccgcgattg caggccgctt ccgaaacttg aaatcaatcc ggaagtaaaa aacatttttg
     6661 atttcaaata cgaagatttt tccattaccg gctacgaccc gcatccacac atcaaagcgg
     6721 tggtagctgt ttaa
//
LOCUS       DAOPKE010000129         6724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12058_length_6724_cov_12.479832, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000129 DAOPKE010000000
VERSION     DAOPKE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12058_length_6724_cov_12.479832"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..538
                     /locus_tag="PKY63_11790"
     CDS             134..538
                     /locus_tag="PKY63_11790"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPF01340.1"
                     /translation="MENRDFDSNKFLKFWSDSSDEDYETMLALFESKRYSWALFVGHL
                     MIEKLLKALFVQVNHDYPPFIHNLLLLAEKCKLDLNDEQKIFFITVTAFNINARYDDY
                     KMSFQKKCTPEFTSIWIEKIKTNRLWIKEQIK"
     gene            514..828
                     /locus_tag="PKY63_11795"
     CDS             514..828
                     /locus_tag="PKY63_11795"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPF01341.1"
                     /translation="MDKRTDKIIRQYLSTVSVQTPGFVTAYLFGSYAKGNQHADSDID
                     IALIIENLQDSDKFDIQVQLMLLASKFDLRIEPHPLSLNDLESDNPFVYEIKKTGIEL
                     RK"
     gene            890..1480
                     /locus_tag="PKY63_11800"
     CDS             890..1480
                     /locus_tag="PKY63_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01342.1"
                     /translation="MGLSFHYSGSIASPELLPELMDEVRDIAKVHHWDVHEFEREFPE
                     GAYGKEEFNQDIYGLVFSPPECEPVWICFLSNGRISSPMHLQFWGDAKEPPESDYLYM
                     ISVKTQYAGIEIHQFIIDLFRYLNKKYFSSFKLQDEGEYWETNDIDVLKANFNKYTSL
                     MNSFTTALEIIPKEYGENLEEYFLRLMKMIQDRGKI"
     gene            1542..1928
                     /locus_tag="PKY63_11805"
     CDS             1542..1928
                     /locus_tag="PKY63_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPF01343.1"
                     /translation="MDEKVKYWVDISDYDLETADAMLQSKRYLYVGFMCHQTIEKIFK
                     AYYTALKSEVAPYSHSLSFLAKSGDFYEQFSEEQKDFLDQIEPLNIEARYPSHKERLL
                     KSLTETKCIDIITKTKELQQWIKAKL"
     gene            1907..2209
                     /locus_tag="PKY63_11810"
     CDS             1907..2209
                     /locus_tag="PKY63_11810"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPF01344.1"
                     /translation="MDKSEAINIVRSYGLLLRKYFPLEKIYLYGSYAKNTYRADSDID
                     VAIVVKHIDGDYFSVTPLLWKLRRQIDDRIEPILIERENDKGDFLSEIQKNGIEVE"
     gene            2633..3709
                     /gene="serC"
                     /locus_tag="PKY63_11815"
     CDS             2633..3709
                     /gene="serC"
                     /locus_tag="PKY63_11815"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPF01345.1"
                     /translation="MKKHNFNAGPAILPRIAIENTAKAVLDLNGIGMSLLEISHRSKD
                     FQAVMDNAVALMKEVMEIPDNYHVLFLGGGASTQFCMIPYNFLKTKAAYLETGVWAKK
                     ALKEAKGLGEVITVASSADKNFSYIPKGWTVPADADYFHITTNNTIYGTEIREDFDCN
                     IPLIADMSSDMCSRPVDVKKYAMIYGGAQKNIGPAGATFVIIKDEFLQQITDKRYLPT
                     MLDYRTHVKDGSMFNTPPCLPIFTIMETMKWLKELGGLKVMEKMNIEKADILYNAIDS
                     SKMFVGTAEKDSRSLMNICFVMNEQYKDKEEAFLEFAKTKGLIGLKGHRSVGGFRAST
                     YNALPKESIQYLVECMQEFEKQNA"
     gene            3879..4802
                     /locus_tag="PKY63_11820"
     CDS             3879..4802
                     /locus_tag="PKY63_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF01346.1"
                     /translation="MTKVLIATDKPFAASAVNGIRNVVETAGFELKLLEKYTDKKQLL
                     DAVADVDAMIVRSDIVDAEVIEHAKNLKIVVRAGAGYDNLDLASCTAKNIVAMNTPGQ
                     NSNAVAELAMGMMLMIVRNKYDGTSGTEVKDKTLGIHAYGYVGKNLARIAKGFNMKIM
                     AFDPFVDKSVMEIDGIIPAVSVEELYKNCQFVSLHIPANDKTKKSINKALVSLMPKGG
                     VLVNTARKEVINEEELLQLFEERPDLRYVSDIAPDNAAAFVEKYAPRVFFTPKKLGAQ
                     TSEANNNAGIAAAEQIVKFIKNGDKTFQVNK"
     gene            4831..5289
                     /locus_tag="PKY63_11825"
     CDS             4831..5289
                     /locus_tag="PKY63_11825"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPF01347.1"
                     /translation="MENKVYNFFRFEDLRVYHKALEYSRWVDEQMKMVESGAVAEYYA
                     KSFSKSAMHIPINIAEGSGRNKSQFVYYLKLAKSCVRECVVFTTVGLNENYLSQEKYE
                     ESRSVLIEISKMVGALIFSLQKGRPKDENDEFEEHNDENSSMTSDEEFDL"
     gene            5311..6558
                     /locus_tag="PKY63_11830"
     CDS             5311..6558
                     /locus_tag="PKY63_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPF01348.1"
                     /translation="MATLKAFKGLRPPKNIAHQVASRPYDVLNSKEARIEAEGNEYSL
                     LHIIKPEIDLAEGIDEHDESVYNKAQENLSKYRKNGWLVQDEKEYLYIYAQTMNGKTQ
                     YGIVGCAGVDDYLNNVIKKHELTRKDKEEDRMKHVRITNANMEPVFFSYPAHAELDNM
                     IMDYAKSNPAEYDFTAADGVGHHFWVIRDEAMIAKIIGIFKTFPAVYVADGHHRTAAA
                     ALVGKEKRGLNPGYNGSEEFNYFLAVHFPDNQLTIIDYNRVVKDLNGMNAAQLLEKLN
                     ENFDVELKGTDIYKPDTLHNFSMYLEGKWYSLTAKHGTYNDNDPIGVLDVTILSELVL
                     DKLFDIKDLRTDKRIDFVGGIRGLGELVKRVDSGEMKVAFALYPVTMKQLIDIADTNN
                     IMPPKTTWFEPKLRSGLVVHSLE"
BASE COUNT         2174 a         1249 c         1440 g         1861 t
ORIGIN      
        1 aatacatgaa taaaacaacg caagttacta acatttaaat gtttacaaca tttatgcttg
       61 ggaaattaat ttagatgcgt ttgccctgaa acatcagttg aatcaatgca aaattatttt
      121 gtatctttgg gtaatggaaa acagggattt tgacagtaat aaatttctaa agttttggtc
      181 tgacagttcg gacgaagatt atgaaacaat gcttgcttta tttgaaagca aaagatatag
      241 ctgggcatta tttgttggcc atttgatgat tgaaaagttg ttaaaagctt tattcgtgca
      301 ggtaaatcac gattaccctc cttttattca taatttatta ttactcgctg aaaaatgcaa
      361 attggattta aacgatgaac agaaaatatt tttcattaca gtgaccgcat ttaatatcaa
      421 tgctcgatat gatgattaca aaatgagttt tcaaaaaaaa tgcacacctg aatttacttc
      481 gatttggatt gagaaaatta agaccaacag attatggata aaagaacaga taaaataatc
      541 agacaatatt tatcaacagt atcggttcag actcctggat ttgtaactgc atatctattt
      601 ggttcctatg caaaaggaaa tcagcatgct gacagcgaca tcgatattgc attaatcatt
      661 gaaaaccttc aggactctga caaatttgat attcaggtac aattgatgtt gcttgcatct
      721 aaatttgatt tacggattga gccgcatcct ttgtcattaa atgaccttga atctgacaat
      781 ccatttgttt atgaaatcaa gaaaaccgga attgagttgc ggaaataatt ccgaaaattc
      841 aaatgtcgat aatatttttg ttattgataa tcaataagcc gatacagcca tgggactaag
      901 ttttcattac agcggcagca ttgccagtcc ggaattgttg ccggaactaa tggatgaagt
      961 gcgcgatatt gcaaaagtgc atcactggga tgttcatgag tttgagcgcg aatttccgga
     1021 aggcgcttac gggaaagagg agtttaatca ggatatttac ggtcttgttt tctcgccgcc
     1081 tgaatgcgaa ccggtatgga tttgctttct gtcgaacggc cgcatcagca gcccgatgca
     1141 ccttcagttt tggggcgacg ccaaggagcc gcctgaaagc gattatttat acatgatttc
     1201 agtgaaaacg caatatgccg ggatcgaaat tcatcagttt atcattgatt tgttcagata
     1261 tctgaataaa aagtattttt catcttttaa attgcaggat gaaggtgagt actgggagac
     1321 aaatgacatt gatgttctga aggcaaattt caataaatac acttcgctga tgaacagctt
     1381 tacaactgcg ctggagatta tcccaaaaga atacggcgaa aatctggaag agtattttct
     1441 tcgattgatg aagatgattc aggatcgcgg taaaatttga tattgctgaa tatgatgtaa
     1501 atatgtctgt ttttttgtat ctttggaata tatttctgac aatggatgaa aaagtaaaat
     1561 attgggttga catatctgat tatgatttgg aaactgcaga tgcaatgctt caaagcaaac
     1621 gctacctcta tgtgggattt atgtgccatc agactattga aaaaatattc aaagcctatt
     1681 atactgcact gaaatctgag gttgcacctt attcgcatag tttatctttt ctggctaaaa
     1741 gcggagattt ttacgaacaa ttttccgagg aacaaaaaga ttttctcgat cagattgagc
     1801 cattgaatat tgaggctcgt tacccatcgc ataaagagcg gttattgaaa agtttgacgg
     1861 aaacaaaatg cattgatatt attaccaaga cgaaggaatt acagcaatgg ataaaagcga
     1921 agctataaat attgtaagaa gctacggtct tttgctcagg aagtattttc ctcttgagaa
     1981 gatttatttg tatggctcgt atgcaaagaa cacgtacagg gcagactctg atattgatgt
     2041 tgctattgtc gtcaaacata ttgatggaga ctatttttcc gtgacacctt tactttggaa
     2101 actaagacgt caaattgatg atagaataga acccatactg attgaaagag aaaacgataa
     2161 aggcgatttc ctgagtgaaa ttcagaaaaa tggaattgaa gtagagtagt attttccgct
     2221 cctgcagatt tttaaattca aggctgggcg tagtctttag agaaaatttc gttgggttta
     2281 gtcttgagat taaacccagc aaataatttt aatacagact acgcccgatg tatataaaga
     2341 gcgctgcagg agcgaaattc gattagtata gtaacataaa ataagcggcg cattgtaaga
     2401 cattagttca caagatatca ttcgctcaca caacagcatt gcgactcaaa aaagtcagct
     2461 cacttaattg acgaaaccgc agtaaaaaat tgatttattt caatatcgat gcaagtggct
     2521 gaacaataga atcatagagc tttgtttaga atggttaaat tttagggtta aagtcctgca
     2581 gatgaaatgt attctatatc tttgctgcga ataaaactaa attcaatttg atatgaaaaa
     2641 gcataatttc aacgccggcc cggctatttt gccccggatt gctatcgaaa acaccgcaaa
     2701 agccgttctt gatctgaacg gaattggcat gtcgctgctc gaaatcagcc accgcagcaa
     2761 ggattttcag gctgtgatgg acaatgccgt tgctctgatg aaagaagtga tggaaattcc
     2821 cgacaattat catgtgctct tccttggtgg tggtgccagt acacagttct gtatgattcc
     2881 ttacaatttt ctgaagacaa aagccgcgta tctcgaaaca ggcgtttggg caaaaaaagc
     2941 gctgaaagaa gccaaaggtc ttggtgaggt aattaccgtt gccagctctg ctgacaaaaa
     3001 cttcagctat attcccaaag gatggacggt tcctgccgat gctgactatt tccacatcac
     3061 aaccaacaat accatttacg gtactgaaat ccgtgaagat tttgactgca atattccgct
     3121 gattgctgat atgtcatctg acatgtgcag tcgcccggtt gatgtgaaga aatacgccat
     3181 gatttacggt ggtgcacaga aaaacatcgg acctgccgga gcaacatttg tcattatcaa
     3241 agacgaattc ctgcagcaga ttaccgacaa gcgttacctg ccgaccatgc ttgattatcg
     3301 cacacacgtg aaagatggaa gcatgttcaa cactcctcct tgtcttccga ttttcaccat
     3361 tatggaaacc atgaaatggc tgaaagaatt aggtggtttg aaagtgatgg aaaaaatgaa
     3421 tatcgaaaaa gccgacattc tgtacaatgc cattgatagc agcaaaatgt ttgtcggtac
     3481 tgctgaaaaa gacagccgtt cactcatgaa catctgcttt gttatgaacg aacagtacaa
     3541 agacaaagaa gaagctttcc tcgagtttgc aaaaacaaaa ggtctgattg gtcttaaggg
     3601 acatcgctct gttggcggat tccgcgcatc gacctacaat gcacttccaa aagaaagtat
     3661 tcagtatttg gttgaatgta tgcaggaatt cgaaaaacaa aacgcataat taaataaaaa
     3721 aagtcccgca ggagatattg attcaatgga atgaagagct tctgcgggat tttgctttca
     3781 tcagaatttt cgatttttca gaataaagtt tgaataaaac aggtgtcggc aaactttatt
     3841 taattaattt tgagacacct gaacaaaaaa taaaaatcat gacaaaagta ttgatagcta
     3901 ctgataaacc ttttgcagca tctgcagtaa acggaattcg caatgtagta gaaaccgccg
     3961 ggtttgaact taagttgctc gaaaaatata cggataaaaa acaattgctc gatgctgtgg
     4021 ccgatgtgga cgccatgatt gtgcgtagtg atattgttga tgcagaagtg atcgaacatg
     4081 caaaaaatct gaaaattgta gtacgtgctg gtgctggtta cgacaatctc gatctggctt
     4141 catgcacagc caaaaacatt gtggcaatga atacgcccgg acagaacagt aatgctgttg
     4201 ccgagctcgc catgggcatg atgcttatga ttgtccgtaa caaatatgat ggaaccagtg
     4261 gtaccgaagt aaaagacaaa actctcggaa ttcacgctta tggctatgtt ggaaagaatc
     4321 tggcccgtat tgccaaagga ttcaacatga aaattatggc attcgatcca ttcgttgaca
     4381 agagtgttat ggaaatagac ggtattattc cggctgtcag tgttgaagag ctgtacaaaa
     4441 actgtcagtt cgtttctctg catattcctg ccaacgacaa aacaaaaaaa tcaatcaaca
     4501 aagcccttgt ttctttgatg cctaaaggtg gtgtgcttgt aaatacagcc cgtaaggaag
     4561 tcatcaacga ggaagaactg cttcagttat tcgaagagcg tcctgatttg cgctatgtga
     4621 gcgatattgc acccgataat gctgcagctt ttgttgaaaa atatgcacca cgtgtgtttt
     4681 tcactccgaa aaaactgggt gcgcagactt ctgaagctaa taacaacgca ggaattgctg
     4741 ctgccgaaca gattgtcaag ttcatcaaga acggcgacaa aacattccag gtaaataaat
     4801 agataatcca tatcaaatcc ccccacagtt atggaaaaca aagtgtataa ttttttccgt
     4861 ttcgaagacc ttcgggtgta tcacaaggcg cttgaatact cacgttgggt tgatgaacag
     4921 atgaagatgg ttgagtctgg tgctgttgct gaatactatg caaaaagctt ttcgaaatca
     4981 gctatgcata ttccgatcaa cattgctgaa ggaagcggac gaaacaaaag tcagttcgtt
     5041 tattatttaa aactggccaa aagctgtgtg agggaatgtg ttgtttttac tactgtcgga
     5101 ctcaatgaaa attatctgag tcaggaaaaa tatgaagaat cgcgctctgt gctgattgag
     5161 atatcgaaaa tggtgggagc gctcattttt tcgcttcaaa aaggaagacc aaaagatgaa
     5221 aatgatgaat tcgaagaaca caacgacgaa aattcatcaa tgacatcgga tgaagaattt
     5281 gatttataaa ctgaaataaa actctgaaga atggctacat taaaggcgtt taaaggactc
     5341 cgccctccaa aaaacattgc acatcaggtt gcatcacgtc cgtacgacgt gctcaattca
     5401 aaagaagccc gcatcgaagc agaagggaat gagtattcgc tgctgcacat catcaagccc
     5461 gaaatcgatc tggctgaagg tattgatgaa cacgatgagt cggtgtacaa caaagcccag
     5521 gaaaacctga gtaaataccg taaaaacggc tggctcgtgc aggacgaaaa agagtatctc
     5581 tacatttatg cacagactat gaatggcaaa actcagtacg gtattgtcgg ttgtgcaggc
     5641 gttgatgatt atctcaacaa cgtgatcaaa aaacacgaac tcacacgaaa agataaagaa
     5701 gaagaccgta tgaaacatgt gcgcatcacc aatgccaaca tggaacccgt gttcttttcc
     5761 tatccggctc atgctgaact cgacaatatg attatggatt atgcaaaatc aaatcctgct
     5821 gaatacgatt tcactgctgc agacggtgta ggacaccatt tctgggtaat ccgcgatgaa
     5881 gctatgattg caaaaattat tggcattttc aaaacatttc cagctgtata tgtagccgat
     5941 ggtcatcacc gtactgcagc cgccgcattg gttggtaaag aaaaacgcgg actgaatcca
     6001 ggatacaacg gaagcgaaga attcaattat tttcttgccg tgcatttccc cgacaatcag
     6061 ctcaccatca tcgattacaa ccgcgtggtg aaagatctga acggaatgaa tgctgctcag
     6121 ttgcttgaaa agctgaatga gaatttcgat gttgaactta aaggaaccga catttacaaa
     6181 cctgacacac tgcacaattt cagcatgtat ctcgaaggta aatggtattc tctgactgcc
     6241 aaacacggca cctacaacga caatgatccg attggcgtgc tcgatgtaac cattctctca
     6301 gaattggtgc tcgacaaact ttttgacatc aaagatctgc gcactgacaa acgcatcgac
     6361 tttgtcggcg gtatccgcgg tctgggtgag cttgtgaagc gcgttgacag tggtgaaatg
     6421 aaagtagctt ttgcactgta tcctgtcacc atgaagcagt tgatcgacat tgccgacacc
     6481 aacaacatca tgcctccgaa aaccacgtgg ttcgaaccga agctccgcag cgggcttgtg
     6541 gtgcactcgc tggagtagta tcggaaatta attttcaaaa aaagcatcgc gcggatttgt
     6601 gattcgtgcg atgtttttat ttgcttttat tagagtaaga aaagagcaat cggtcacgcg
     6661 tcaacgtaaa acgctgcagg cgggagacgc gcgcccagta agggtagttc gtaaagtaga
     6721 aagt
//
LOCUS       DAOPKE010000130         6712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12099_length_6712_cov_15.238846, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000130 DAOPKE010000000
VERSION     DAOPKE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12099_length_6712_cov_15.238846"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1561)
                     /locus_tag="PKY63_11835"
     CDS             complement(197..1561)
                     /locus_tag="PKY63_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPF01349.1"
                     /translation="MEYAAIIQRQRDFFHSNVTKDIRFRAEQLRKLKSLLQANEKRLC
                     EAIYADFRKGEFETYATELGILYNDIGESIKKMRRWSRTKRVLTNIPNMPARSFIMPE
                     PYGVCLVIGAWNYPYQLSFAPAIAALAAGNTVILKPSELSAHTSALIAELINTSFDPG
                     LFHVIEGGVPETTALLKEKFDKIFFTGSVPVGNIVYQAAAKNLTPVTLELGGKSPAFV
                     FADTNLDVTARRLVWGKFLNAGQTCIAPDYVLVENKIKEKFLHAIKAQIDEFRYSFEN
                     KNHVQIINERNFDRLVNLIDHSKVIYGGEHDRDERYISPTILNDLDFSHPVMDDEIFG
                     PILPVIGFDDLDKMIVEVKNRPKPLACYVFTKSKTLRKKILHEISFGGGAINDTIMHI
                     SNPRLPFGGVGHSGTGSYHGEAGFRTFSHYKSVMYKPFRFEPNLKYPPYTEGKMKWLK
                     RFLR"
     gene            complement(1815..3965)
                     /gene="scpA"
                     /locus_tag="PKY63_11840"
     CDS             complement(1815..3965)
                     /gene="scpA"
                     /locus_tag="PKY63_11840"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPF01350.1"
                     /translation="MRPDFKNIKLADFKGNTPYSAWEKENGIAPEIDTPEKIKIKPSF
                     GEDDLANMEHLSYGAGLPPFLRGPYSTMYVMRPWTVRQYAGFSTAEESNAFYRRNMAA
                     GQKGLSVAFDLATHRGYDSDNERVLGDVGKAGVAIDSVEDMKILFDQIPLGEVSVSMT
                     MNGAVLPVMAFYIVTAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPVPSMKIIADI
                     FEYTSKLMPKFNSISISGYHMQEAGATADIEMAYTLADGLEYLRTGIKAGIPVDKFAP
                     RLSFFWAIGMNHFMEIAKMRAARMLWAKIVSKFEPKSAKSLALRTHSQTSGWSLTEQD
                     PFNNVARTAVEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIFIQEETDICR
                     AIDPWAGSYYVEFLTKQLAESAWKLIEEVEELGGMAKAIETGIPKMRIEEAAARKQAR
                     IDSKRDTIIGLNKYRLEKEDPIETLEVDNTAVRESQIARLKEIRLKRDNAAVAAALEA
                     ITKACETGQGNLLELAVDAARKRATLGEISDAMEKVFGRYSAVIRSISGVYSSESQSN
                     ESFKKATEMAAEFAALEGRRPRILIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQ
                     TPAEAARMAVENDVHILGVSSLAAGHKTLVPQVIEELKKLGREDIMVIVGGVIPPQDY
                     DFLFKAGALAVFGPGTVISDAAIKMLEIMIAARK"
     gene            complement(3979..5829)
                     /locus_tag="PKY63_11845"
     CDS             complement(3979..5829)
                     /locus_tag="PKY63_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPF01351.1"
                     /translation="MSDNKLFSEFPPITKEAWEQLINEDLKGADYEKKLVWKPLDGFS
                     VQPYYMAEDVKSAMPANHPGQAPFIRGYHASGNTWKIIQQIDTTNTAEANSFACNALN
                     RGADGVSFRLDFSDKQEEMTALLKDIDLTKSAMHFHSHHSYSILAELLKVEAASKGVQ
                     NSNLSGSFNFDSFGYYLLKGSYYNSHEDNMNELKCLIENVGKCLPGYKVMNVNGQHFA
                     NAGASAVQELGFAIASAHEYLQEMLNKGLKAEDVLSKMKITLSVGPSYFLEIAKFRAA
                     RWLWASVASQYSSNPELQKITIHGISSLYNKTLYDLYNNMLRNTTEAMSAALGGADEI
                     TILPHDFILGKESEFGDRIARNVQLLLKEESHFADVADPAAGSYYIESLTQSVAEYAW
                     KQFQMIEENGGFRKAMESGLIKSEIEKTVAKREKDIATRKMVYVGVNNYPNTNEQFAE
                     AAAFIKAPNAEGNALNMQRGAWSFEQIRMRTDKHVAAGNKRPRVTMLQFGNIAMRNAR
                     AIFATNFFGMAGYEITTILNDDSIQDGVKKALDEKPGIIVLCSSDEEYPTLGVEYLKV
                     LRNTNIPLVLAGSPGDNEAMYREAGVHSFVHMRTAALEALENYQTVLSVQ"
BASE COUNT         1660 a         1611 c         1541 g         1900 t
ORIGIN      
        1 ttgaagaagt cttgtttttg gacatcctct ttgtttttct attttctaaa gacatctgac
       61 tcctctggag tctgccatga aatttcatct aaataatatt tgaaacttca ttatttcagt
      121 tcaaacgcat ccatataatt ctttacaata aacatatcgc catcgcggaa ttcaagctcg
      181 agggagatgt cttcgtttat ctcagaaacc gtttcagcca tttcattttg ccttcggtgt
      241 agggcggata tttcaggttg ggttcaaaac ggaatggctt atacataacg cttttgtaat
      301 gcgaaaaagt acggaagccg gcttcgccgt gatagctccc ggtgccgctg tggcccacac
      361 cgccgaatgg cagccgtgga ttcgagatgt gcatgatggt gtcgttgatg gcgccaccgc
      421 caaacgaaat ttcgtgtaat atttttttgc gaagcgtttt gctttttgtg aaaacatagc
      481 aggcaagcgg ctttggacgg ttcttgactt caacaatcat tttgtccaga tcatcaaaac
      541 caatcaccgg aagaataggg ccgaagattt catcgtccat caccggatgc gagaagtcaa
      601 ggtcgttgag aatggtcggg ctgatgtagc gctcatcgcg gtcatgctcg cctccgtata
      661 ttacttttga atgatcaatc agattcacca gccggtcgaa atttcgctca ttgatgatct
      721 gcacatggtt tttattttcg aacgaataac ggaattcatc gatttgtgcc ttgatggcgt
      781 gcagaaattt ttcctttatt ttattctcaa ccagcacata atcaggcgca atgcaggttt
      841 gaccggcatt gagaaatttt ccccatacca gacgtctggc ggtgacatcg agattggtat
      901 cggcaaatac aaaagcagga ctttttccac ccagctccag cgtgaccggt gtcaggtttt
      961 tggcagcagc ctgatacacg atgttgccaa caggaacgct gccggtgaaa aatatcttgt
     1021 caaacttttc cttcagcaag gccgttgttt ccggcactcc gccttcgatt acgtgaaaca
     1081 ggcccggatc gaaacttgtg ttgatcagct ccgcaattaa tgccgaagtg tgtgctgata
     1141 attcgcttgg tttcaggata acggtgtttc cggcagccag tgcggcaatg gcaggtgcaa
     1201 acgaaagctg atacggataa ttccatgcgc cgataacaag acaaacacca taaggctcgg
     1261 gcatgatgaa cgatcgtgcc ggcatgtttg gaatgttggt caaaacgcgt tttgtgcggc
     1321 tccagcggcg cattttcttt atagattcgc caatgtcgtt gtataaaatt ccaagttcgg
     1381 tggcgtaggt ttcaaattct ccttttctga aatcggcgta aatggcttcg cagagccgtt
     1441 tttcattggc ctgcagaagt gatttcagtt tgcgcaattg ctccgccctg aagcggatat
     1501 ctttggtgac attcgaatga aagaagtcgc gttgccgttg aatgattgct gcatattcca
     1561 tgatttcaaa gatagaaaaa atttagtctc cccgacattt taccgtcagc gaaaagggat
     1621 ttacaatacg tcattgctga cgtggaattt tatctgactg gcaaggcaga cgtcggggag
     1681 tttgtgcccg gagcttcaga actggtgcac agtcacgcca tgcaccatgc tctgtgcttt
     1741 gtactgcatg ccttatgcac aaaagcacca tcatctttac agacaatgat gctttcacaa
     1801 aaattgaaat gcatttattt tctagcggca atcataatct cgagcatctt gatggctgca
     1861 tcggagatga cggttcccgg gccaaacaca gccaaagcgc cggctttgaa caggaagtca
     1921 taatcctgcg gcggaataac tccacccaca atcaccatga tgtcctcgcg tccaagcttc
     1981 ttcagttctt cgatcacctg cggcaccagc gttttgtggc cggcggcaag actcgataca
     2041 ccgagaatat ggacatcgtt ctccaccgcc atacgagcgg cttcagccgg agtctgaaac
     2101 agcggaccaa tgtcaacatc gaatccgata tcggcatagc cggtggctac aaccttggct
     2161 ccgcggtcat gtccgtcctg tcccatcttt gcaatgagga tacgcgggcg gcggccttcg
     2221 agtgcggcaa attcagcggc catctctgta gctttcttga agctttcgtt tgattgactt
     2281 tcggatgaat acacgcctga tatacttctt atgacagcgg aataacggcc gaaaactttc
     2341 tccatggcgt cgctgatttc gcccagcgtt gcacgcttgc gggcggcatc aacagccagt
     2401 tccagcagat ttccctgtcc ggtttcacat gcttttgtta ttgcttcgag cgctgctgca
     2461 acagcggcgt tgtcgcgctt caacctgatt tctttcagtc ttgcaatctg cgattccctt
     2521 acagcagtgt tgtccacttc gagggtttcg atcggatctt ctttttcaag acgatatttg
     2581 ttcagaccaa tgatggtgtc gcgcttgctg tcaatgcggg cctgcttgcg ggctgcagct
     2641 tcttcgatcc tcattttcgg aataccggtt tcaatggctt tggccatgcc gccaagttct
     2701 tccacttctt cgatgagttt ccatgcgctt tccgccagtt gtttggtcag aaattccaca
     2761 taataggaac cagcccatgg atcaatagca cggcagatat cggtttcttc ctgaatgaaa
     2821 atctgtgtgt tgcgagcaat gcgtgccgag aaatcggtgg gcaatgcaat ggcttcatca
     2881 agcgcattgg tatgcagact ctgtgtgtgt ccgagagcag cagccattgc ttcaacagcc
     2941 gtacgggcca cattattgaa cggatcctgc tcggtcagcg accagcccga tgtctgcgag
     3001 tgtgtacgca atgccagcga tttggcgctt ttcggttcaa acttgctgac aatcttggcc
     3061 cacaacatac gtgcagcgcg catcttggcg atttccataa aatggttcat gccaatcgcc
     3121 cagaaaaacg acagacgcgg tgcaaacttg tcaaccggaa tgccggcttt gataccggtt
     3181 ctcagatatt caagtccgtc ggccagcgta tatgccattt cgatatcggc cgtagcaccg
     3241 gcttcctgca tgtgatatcc actgattgaa atgctgttga acttgggcat cagcttcgac
     3301 gtgtattcga agatatcagc aatgattttc atcgacggca ccggcggata gatgtaggtg
     3361 ttacgcacca tgaattcctt cagaatgtcg ttctgaatgg ttccggccag ctgttcgggt
     3421 ttcacaccct gttcttcggc ggttacaatg tagaaagcca tcacgggcag tacagcgccg
     3481 ttcatggtca tcgacaccga cacttcgcca agcggaatct gatcgaagag aattttcata
     3541 tcctccaccg aatcgatggc cacccctgct tttccaacat cacccagtac gcgctcgtta
     3601 tcgctgtcgt agccacgatg tgttgccagg tcgaatgcca cagaaagacc tttctgtcca
     3661 gccgccatat tgcggcgata aaaggcattt gattcttcgg cggtgctgaa gccagcgtac
     3721 tgacgcacag tccagggacg catcacatac atggtgctgt acggtccgcg aagaaacggc
     3781 ggaaggccgg caccatagga caaatgctcc atattggcca ggtcatcttc gccgaaggaa
     3841 ggttttattt tgattttttc cggcgtatca atttccgggg caatcccatt ttccttttcc
     3901 catgcggaat agggagtatt gcctttaaaa tctgcgagtt ttatattttt aaaatccggt
     3961 ctcattttca ctcctttttt attgaaccga taaaaccgtc tgataatttt caagcgcttc
     4021 gagagcggca gtgcgcatgt gcacaaagct gtgaacaccg gcttcgcggt acatggcttc
     4081 gttgtcaccc gggctaccag ccagcaccaa tggaatattg gtattgcgaa gtactttcag
     4141 atattcaaca cccagtgtcg gatattcctc atcgctgctg caaagcacaa tgattcccgg
     4201 tttttcatcc agcgcttttt ttactccatc ctgaattgaa tcatcattca gaattgtagt
     4261 gatttcatag ccggccatac caaagaaatt ggtggcgaaa atcgcacggg cattgcgcat
     4321 ggcgatgttg ccaaactgca gcatggttac acgcggacgc ttgttaccgg cggccacatg
     4381 tttgtcggtg cgcatacgaa tctgttcgaa gctccaggcg ccgcgttgca tgttcaatgc
     4441 atttccttcg gcatttggag ccttaatgaa agcagctgcc tctgcgaatt gctcgtttgt
     4501 attcggataa ttgttgacac ccacatatac catcttgcgc gtagcaatat ctttttcgcg
     4561 tttggctacg gtcttttcga tttcagattt gatgagtccg ctttccattg ctttgcggaa
     4621 tccaccgttt tcctcaatca tctggaattg cttccatgca tattcagcca cgctctgtgt
     4681 cagtgattcg atataatatg aaccagcagc aggatcggcc acatcggcaa aatgcgattc
     4741 ttctttcagc aacaactgta cgttgcgtgc aatgcggtcg ccgaattcgc tttccttacc
     4801 gagaataaaa tcatgcggca gaatggtgat ttcgtcggct ccgcccagcg cggcactcat
     4861 ggcctcggtg gtattgcgca gcatattgtt gtacaaatca tacagcgttt tgttgtacag
     4921 cgatgaaatg ccatggatgg ttattttctg taattccgga ttgctgctat actgcgaagc
     4981 aacggatgcc cacaaccagc gtgcagcgcg gaatttagct atttcgagaa aatatgacgg
     5041 accgaccgaa agggtgattt tcattttcga caatacgtct tcggctttca gtcccttatt
     5101 cagcatttcc tgcagatatt catgtgccga agcaatggca aagccgagtt cctgcacagc
     5161 agaagccccg gcatttgcaa aatgctgacc atttacattc atcaccttgt atcccggaag
     5221 gcattttcct acattttcaa tcaggcattt gagctcattc atattatcct cgtggctgtt
     5281 gtaatacgag cccttgagca gataataacc aaacgagtcg aaattaaaac taccgctgag
     5341 gttactgttt tgaacgcctt ttgaagcagc ttcaactttc aggagttcag ccagaatgct
     5401 gtagctgtga tgtgaatgaa aatgcatggc agattttgtc agatcgatgt ctttgagcag
     5461 cgcagtcatt tcttcctgct tgtcgctgaa atcaagtctg aacgagacac cgtcggcgcc
     5521 acggttgagc gcgttgcagg caaagctgtt ggcttcggcc gtgttggtgg tgtcaatctg
     5581 ctgaatgatt ttccaggtat tgccgctggc atgatagccg cggataaagg gagcctgacc
     5641 gggatggttg gcgggcatgg cgctttttac gtcttcagcc atataatacg gctgcacgga
     5701 aaaaccgtcg agaggtttcc acaccagctt tttctcataa tcagcaccct tgagatcttc
     5761 gttaatcagc tgttcccacg cctctttggt aatcggtgga aactcgctga acaatttgtt
     5821 gtcactcatg tttttgctgt tttttcgcgc tgcaaaggta attgttttta tatatcagaa
     5881 tatgttatct gctcaaatat gctattatca acaccgctga accacaaaaa tcagccgaaa
     5941 atcatagtta cacagagttc cacggagtta aaagaggaaa agcaaaagcc gattcatata
     6001 aacacaacga gttgtgtttc aataattaca gcaaattcaa tatgcaagct tacttgcaat
     6061 gcttaatttg ctgtaattaa taacggcttt tgcctgaacg aatcaaactc tgcgtgcctc
     6121 tgtgcgtact cctatgcaac atctggaatc ccaggaagaa tcgccttttc agcgatacag
     6181 gcaaaaaaat ccgaaaagta taacaattat ttataaaaat ccgaaacgag attgattata
     6241 tttagtgatt gttttttgcc tttttgcgta ttaagtctgc tcttttatct aagtaaattt
     6301 tttaaggaca aatcgcttga tccattttcc ccactgaaaa acgtttacga tggaaaagaa
     6361 aaaaacaaaa aaattcccac tgtcatcaaa aacaaagcta ctgtaaaagg atggcttgaa
     6421 aatcttgaga tcagcacagg ggtttttatc caaaaggata gccagctttt gatgtgtgaa
     6481 agcaattatc tgacgatttt cagactgtgt gcgaaagttg ctgttttgaa aaatcacacg
     6541 tcgtagtgtt gcggcaggtt gtccccgaca ttctatcctc accgtaaaag ctgctgtcat
     6601 cctgagacca ccagcaaaac gcgttttgca acctaaatga aaaatgacac tttaaattgc
     6661 tgtcatcctg agtttcgctc ccgtagcgtt tcttgcgaaa tagcttgcga ag
//
LOCUS       DAOPKE010000131         6675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12209_length_6675_cov_11.621752, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000131 DAOPKE010000000
VERSION     DAOPKE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12209_length_6675_cov_11.621752"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..1044)
                     /locus_tag="PKY63_11850"
     CDS             complement(397..1044)
                     /locus_tag="PKY63_11850"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HPF01352.1"
                     /translation="MREKRKIPIKSIPKFAILVDGECEYWYIQMLKRNEKVRHANIEP
                     QIPQRKKLSELYQKVIELALHYDKVFWLIDLDVVIKESKEKKKGNRRPLDELKQYVET
                     IAKNQENVTIIINNPCLEFWFLLHFEDTVLYYDNCSKVIRKLKNQPLVSDYNKSCNYF
                     TNKNNDIYKRLKPLLKTAIVHSKKAKAFDFKNPCMGLSEMAQLFEIPELKSILKQ"
     gene            complement(1044..2339)
                     /locus_tag="PKY63_11855"
     CDS             complement(1044..2339)
                     /locus_tag="PKY63_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01353.1"
                     /translation="MIIEFSVQNFGPIKEKQVLSFEPEKSSHLEDYYLIKAAPGLKLL
                     KLGLIYGPNASGKTTILKALDFLRNLVLEPAEKKTNTLNFEPFLLDAKTPAQHTQLSI
                     SFIQKNVRYHYVVEFNKKSIVREQLDFYDPKKANVFVRETDIKNQFSTITFGSKLKGM
                     AASEKALTANTLWNNTVLGGFLKTNIECRELQDATDWFKNYLKPIVLPKTNLEDYVTS
                     LIDRAEVNKNHVLQILKNADFNISDIIIDKQKAAVPEGLWEYFVEKGSVSKERIIELK
                     QKETITILNLAFEHTVKNKKYRLPDNAESRGTMRYYGFAGLMSMLINSSKAIPIDELE
                     ASLHPDLFVHFLLTFLMNSKKSQIIATTHNREILNNKDIFRNDTIWFTDKSEDSATKL
                     YSLADFDSSVIRDTSNVYNAYKIGKLGAVPDLGDFQLSE"
     gene            complement(2738..5167)
                     /locus_tag="PKY63_11860"
     CDS             complement(2738..5167)
                     /locus_tag="PKY63_11860"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPF01354.1"
                     /translation="MSQESQETMKQFASEEEEKTIDFRMLFYRILRQWYIVALCVLIG
                     LGSAWFYLRKAPRLYQGSTTVLVQDKQNKLMDAQKLMGLDIGQASTTVENEQQILLSK
                     NMSQEAVKHIDWQVSYFISGRIRTNTIYAVRPYVVVIDTLHPQVAGASFDIKFKDDHS
                     FVLTSNQNGGRLYDYRVNDYYLRQGNYIDLPSIQYEKEHRFGDTITTSNFRFVIMPGN
                     AIPSTYARGDLAFRLNNLQAYSDNLNKGLSIEPTSKTSSVLRITSQYYQPEILKDYLN
                     ALTAVYIESGLNNKNSVAVKTIEFIDSELESIAEELSNAESDLESYRKENQIMSLDNE
                     AAAIFDQMREIDKQKAELLLKNKYYNYLRDYVEMDRDLESIVVPSSMGIEDAVTASLI
                     QELTRLYNERSALVKSAGEKNPAVQKINAQIEQTQQNILENTKNIIQLNNMSISEMER
                     RSGSVNARVSLLPTNQRNLLGFEREFNLYNELYVFLLQRRAEAKITAATNMPDNSVLD
                     VCEKVVQIKPKNSMVYLIAFVMSLLVALGIIFLPGWLKNTFEYPREIEELSTFPSAGS
                     IMHYEGNDELVFRSAPRSVVAESFRNLRTNINSMSKNKSGFSIVITSDLPAAGKTFIS
                     VNLGQAFAVAGKKTLLIGADMRKSRLFELIALPKSPGLSEYLTGNASIDDILRVCTEN
                     SNMTVITAGKVPFNPAELLESPEMDSLLAHVKNVYDIIIIDTPPMAAVTDANILVKNS
                     DALAVVVRLRKTRKNILRDLLKELRMRKLNNTVLVINDVPRQKRSGYGYGYGYGYGYG
                     YGYGYIEDDKA"
     gene            complement(5278..5667)
                     /locus_tag="PKY63_11865"
     CDS             complement(5278..5667)
                     /locus_tag="PKY63_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPF01355.1"
                     /translation="MKVEDETMNINRKFIFYLAVAGLVVMNFLPINGSKDDPPLFIFG
                     LRHDLWLHIIAFVVLTFLAAWAFRLSFRKQGLMCLFVPLSFIALAAGLEYAQMLVPNR
                     NVAWDDFYFSSIGAGLGSVTMWTNRRG"
     gene            complement(5745..6512)
                     /locus_tag="PKY63_11870"
     CDS             complement(5745..6512)
                     /locus_tag="PKY63_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPF01356.1"
                     /translation="MKKYTHLFLISAALLMAGCVPQKKILLYQGVDGSTVAKTQTPPQ
                     YHVQRGDVLYIDVKSVDDKATSFINGPITAQTASQQMLSEQTLYFKGYEVGSNGAIQL
                     PFIDSICVAGLTMGEIAKLLVDSLSGYIFDPVVSVKLSNFRVAVNGEVSTPGSFAFYS
                     PTVTIFDVLALARPTDFANRKEVVITRKTGSDSLLVDRIDLTNKDVLSSPYYYMQPGD
                     QLYFEPLKAKQYGFTNFPYSLVLSIISTSLVVYTLFK"
BASE COUNT         1893 a         1475 c         1323 g         1984 t
ORIGIN      
        1 gcggaataaa ttccacgact gtaacgattg taacgtgtaa cgaaaggaaa tacgaaatac
       61 gagatatgat gtggaaatgt gcggctccgc cgatgtggtg ccttcacgta aaacgtgaca
      121 agtcggcgta tgtgggaata tgagagacgt tatgagatac gaaatacgag atacgaaatg
      181 ccaatttgaa aatttgaaaa ttcggcaatt tgaaaatacg aaatacgggt taggcgatag
      241 aaaatacata tgtggcgctc aagtgaatcg tgacaagttc gcgcatgcgg ttttttttgc
      301 gcttcgcttg aaagatttat gtgagcacgg agtttatccc gctggaagcg gggtgctgag
      361 cgggggcggt ttaacataaa ttaatttaaa ttcgcatcac tgtttaagga tgctctttag
      421 ttccggaatt tcgaacagct gtgccatttc tgacaggccc atacaagggt ttttgaaatc
      481 aaatgccttt gctttttttg aatgcacgat ggctgttttc agcaatggtt tcaacctttt
      541 ataaatatcg ttgtttttgt ttgtgaaata attacacgat ttattgtaat cggaaacaag
      601 cggttgattt ttcaattttc ggattacttt agagcaattg tcataataca ggacagtgtc
      661 ttcgaaatgt agcaggaacc agaattcaag gcaaggattg tttattatga tggttacatt
      721 ttcctggttt ttagcaatgg tttcaacata ctgttttaat tcatcaagtg gccgtctgtt
      781 gccttttttc ttttcttttg attctttaat aaccacatca agatcgataa gccagaacac
      841 cttgtcgtaa tgcagcgcca gttcaatgac cttttggtat agctcactca atttttttcg
      901 ctgtggaatc tgcggttcaa tatttgcgtg ccgtactttc tcatttcttt tgagcatctg
      961 aatgtaccaa tactcgcatt cgccatcgac gagaatagca aatttgggaa ttgacttaat
     1021 ggggatcttt cgtttttctc tcattattct gaaagttgaa aatctcccag gtcgggcaca
     1081 gcaccaagtt ttccgatttt ataagcatta tagacattgc tggtgtcgcg gataacggag
     1141 ctatcgaaat cggccagcga atagagcttt gtagcagaat cttctgattt atcggtgaac
     1201 cagatggtat cgtttctgaa aatatctttg ttgttcaata tttcgcgatt gtgcgtagtt
     1261 gcaatgattt gcgatttttt tgaattcatc agaaaagtga gcaaaaaatg aacaaacaaa
     1321 tcgggatgca atgatgcttc cagctcatcg atggggattg ctttggagct attaatcagc
     1381 atactcataa gacctgcaaa accgtaataa cgcattgtac cacgcgattc ggcattatcg
     1441 ggcaaacggt attttttatt tttcacagta tgctcaaatg ccagattaag aattgttata
     1501 gtttctttct gctttaattc aataattctc tcttttgaaa cgctcccttt ttccacaaaa
     1561 tactcccaaa ggccttccgg aacagcagct ttttgcttat caataataat atcagaaata
     1621 ttaaagtctg catttttcaa aatctgcagc acatggttct tattaacctc tgcccgatca
     1681 atgaggcttg ttacataatc ttccagattt gtttttggaa gaactatggg ttttaaataa
     1741 tttttaaacc agtcggtggc atcttgtagt tctctgcact ctatgtttgt tttcagaaaa
     1801 ccgccaagca cggtgttgtt ccagagggta ttggctgtca gcgctttttc actggcagcc
     1861 atgcctttga gtttacttcc aaatgtaatt gtgctgaatt gatttttaat atcggtttcc
     1921 ctcacaaata cattggcctt tttgggatca taaaaatcaa gttgttctct tacaattgat
     1981 tttttattaa actcaactac gtaatgataa cgaacatttt tctgaataaa agaaatagac
     2041 agctgagtat gctgagcggg tgttttagca tcgagcagaa acggctcaaa attcagcgta
     2101 ttcgtttttt tctctgctgg ctccaaaacc agattgcgaa gaaaatcgag cgctttgaga
     2161 atggtggttt tgccagatgc attgggcccg tatatcaatc caagtttgag cagtttcaat
     2221 cctggcgccg ccttgataag gtaataatct tcgagatgag aagacttttc cggctcaaac
     2281 gataagacct gcttttcctt aatcggacca aaattctgaa cactgaattc gataatcatt
     2341 tcgcttgaat tttgtaatga atctgcaaat ataaacaaat aaaatcaatt ttcaacactt
     2401 tacaatcgta ttttgtaatt catttacaat ttagacacat aaaaacacac tgaagactat
     2461 tttatcaagc acactccgtt cgtggccttt gttgaaaaaa tttgagaggt gggatttaat
     2521 ttttttgagc atgggagaga ttaggcgtta ggcattaggc gagaggcgtt atattatgcg
     2581 ctcacgcgaa atcacgtaaa acagcttgtc acgttttacg tgatgacaag ctgtgacaag
     2641 ttcgcgcggg agaagaaaga tcgatgtggc gcgtgggcgt tgatgcgcgt gggcgtagtt
     2701 tcgcatcatc gcattatcgc attatcgcat catcgcatca tgccttatcg tcctcaatat
     2761 agccgtagcc atagccgtag ccataaccgt agccatagcc atatccatat cccgaccgct
     2821 tctggcgcgg aacatcgtta atcacaagca cggtattatt gagttttcgc atgcgtaact
     2881 ctttcagcaa atcacgtaaa atattttttc tggtcttgcg aagccgcacc acaacagcca
     2941 aagcatcgct attcttcact aaaatattcg catcagttac ggcagccatt ggcggagtat
     3001 cgataataat aatatcatat acatttttca catgcgccag taaagaatcc atttcaggac
     3061 tttcaagcag ctcggccgga ttgaaaggaa ccttccccgc ggtaatcacg gtcatattgc
     3121 tattctcagt gcaaacccgc agaatatcat cgattgaagc atttcctgtg agatattcac
     3181 tcaggcccgg acttttcggc aatgcaataa gctcaaacaa acgcgacttg cgcatatcgg
     3241 cgccgataag caaggttttc tttcctgcaa cagcaaaggc ctggcccaaa ttgaccgaga
     3301 taaaagtctt tccggccgcc ggcaaatccg atgtgataac aatggaaaat ccacttttat
     3361 tttttgacat cgaattgata tttgtacgca ggttcctgaa actttcagcc acgactgagc
     3421 gcggtgcact gcgaaaaacc aactcatcat ttccttcata atgcataatg gaacccgcac
     3481 tcggaaacgt tgacaattcc tctatttcac gcggatactc aaacgtattt ttcagccatc
     3541 caggcaggaa aataatgcca agagccacca gcaacgacat tacaaaagca atcagataga
     3601 ccatgctgtt ttttggtttt atctgaacca ctttttcaca cacatcaagc accgaattgt
     3661 cgggcatatt tgtggccgcc gttattttgg cttcagctct gcgttgcaga aggaatacat
     3721 acaattcgtt atacagattg aattcacgct caaatccaag cagattgcgc tggttggtcg
     3781 gcaacagaga aacacgggca ttcacgcttc ctgagcgcct ttccatttcc gaaatgctca
     3841 tgttgttgag ctgaatgatg ttttttgtat tctctaaaat attctgctga gtctgttcaa
     3901 tctgtgcatt gattttctgt accgcaggat ttttttcgcc ggcacttttt accaacgccg
     3961 aacgctcgtt gtagagccgt gtgagctcct gaatcaacga agccgttacc gcatcttcaa
     4021 tacccatgct gcttggaaca acaatgcttt ccagatcacg atccatttca acataatcgc
     4081 gcagataatt gtagtatttg tttttcaaca gcaattccgc tttctgctta tcaatttcgc
     4141 gcatctgatc aaaaatggca gctgcttcat tatcgagact cataatctga ttttccttac
     4201 ggtatgattc caggtccgac tcggcattgc tcagctcttc ggcaatcgat tccagctcgg
     4261 aatcaataaa ttcaatggtt ttgacagcaa cgctgttttt attatttaat cccgattcaa
     4321 tatatacagc agtcaatgca tttaaataat ctttcaggat ttccggttga taatattgag
     4381 atgtaattcg caatactgaa gaagttttcg aagtgggttc aatggaaaga cccttgttca
     4441 gattgtcgga atatgcctga agattattaa gccggaaagc aaggtcacct ctggcatatg
     4501 tagatggaat ggcatttccg ggcatgatta caaaacgaaa atttgaagtg gttatggtat
     4561 ctccaaagcg atgttctttt tcatattgaa tacttggcag gtcgatgtaa ttgccctgtc
     4621 gcaggtaata atcgttgacc cgatagtcat agagccgccc gccattttga tttgatgtca
     4681 gaacaaatga atggtcgtct ttgaatttga tatcgaaaga agcaccggcc acctgcggat
     4741 gaagagtgtc aattacaaca acataaggcc tcacagcata gatggtgttg gtgcggatac
     4801 ggccagatat gaaataactc acttgccagt caatatgctt cacagcttcc tgcgacatat
     4861 tctttgaaag caatatctgt tgctcatttt caacggttgt tgaggcctga ccgatatcga
     4921 gacccataag tttctgagca tccatgagct tattctgctt atcctgcacc aatacagtgg
     4981 ttgaaccctg ataaagccgc ggcgccttgc gcaggtaaaa ccaggcagat ccaagtccaa
     5041 taagcacaca aagcgctaca atataccatt gccgcaaaat cctgtaaaac aacatacgga
     5101 aatcaatggt cttctcttct tcttccgaag caaattgctt catcgtttcc tgactttcct
     5161 gagacatatc ttgattttta aaaattctta atctttattt ctctgccgca ggcagcttaa
     5221 cttttgcctt aacctggctg ttgaccttgt cctcgacctt attaacagtg catcccctca
     5281 gccccgccga ttcgtccaca tcgtcacact ccccaaccct gctccaatcg acgaaaaata
     5341 aaaatcgtcc cacgctacat tgcggtttgg cacaagcatt tgtgcgtatt caagacccgc
     5401 cgccaatgcg ataaatgaca gcggcacaaa taaacacatc agtccttgtt tgcgaaacga
     5461 cagacggaaa gcccatgctg caagaaaggt cagcacaaca aatgcaataa tatgcagcca
     5521 caaatcgtgt cgaagcccaa aaataaataa aggcggatcg tctttagaac cgttgatcgg
     5581 caaaaaattc atcactacca agccagcaac tgcaagataa aatatgaatt tgcgattaat
     5641 attcattgtc tcatcctcaa ccttcatatt cctgccgcag gcagctcatc ctcggcctta
     5701 acctcatcct taacctcagc cttaacctca gccttaactt taacctattt aaacagcgta
     5761 taaaccacca gacttgtcga aataatcgac agtaccagac tatacgggaa attcgtaaaa
     5821 ccgtactgtt tcgctttcag cggctcgaaa tacaactggt cgcccggctg catgtaataa
     5881 tatggggaac tcaatacatc tttattcgtc aaatcaatcc gatcaaccaa aagactatca
     5941 ctgccagtct ttcgtgtaat cactacttct ttgcgattgg caaaatctgt tggtcgggcc
     6001 agagccagaa catcaaagat cgtcaccgtt ggtgaataaa atgcaaatga gcccggtgtc
     6061 gagacttcac cattgactgc cacacggaaa ttggacaatt tgaccgatac caccggatca
     6121 aaaatatacc cgctcagact gtccaccagc aactttgcaa tttcgcccat ggtcaatcct
     6181 gccacgcaga tgctatcgat aaaaggtaac tgaatggcgc cattggaacc cacttcatat
     6241 cctttgaaat acagcgtttg ttccgaaagc atctgttgac tggcagtctg cgccgtaatc
     6301 ggcccgttta taaacgaagt agctttgtca tccaccgatt ttacatcaat atacaacaca
     6361 tcgccgcgct gtacatgata ttgaggtgga gtctgtgttt tggcaaccgt cgatccatct
     6421 acaccctgat atagcaggat ttttttctgt ggaacgcaac cggccatcaa cagtgcagcc
     6481 gaaatcagaa aaagatgtgt gtattttttc attgaaaata atttacttgt gtctgtactc
     6541 aacgaagtct attctctcag gttaagatta agattaaggt taagtggctt tgcctcagct
     6601 gaaagctgca aagcctttgt ctcaacctta gcctggctgt ttcccctgct gcaagcagct
     6661 caacctcaac ctcaa
//
LOCUS       DAOPKE010000132         6560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12507_length_6560_cov_10.980630, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000132 DAOPKE010000000
VERSION     DAOPKE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12507_length_6560_cov_10.980630"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..607)
                     /locus_tag="PKY63_11875"
     CDS             complement(128..607)
                     /locus_tag="PKY63_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01357.1"
                     /translation="MKKTFLFFWVFIANAMVLYGSDYSLKRVEMYLVPKNKSYRIPIS
                     IDNIKKRHEIFVVRYNVELLSSKIFDFNAFKSQLENYPLTQDTSCLSPRILVDLIFRN
                     KIFFKKIAIYFSPEGPFYFNGNWREMNAQLFYYLFIGFDEYLISTDAHENARKLLRE"
     gene            1080..3977
                     /locus_tag="PKY63_11880"
     CDS             1080..3977
                     /locus_tag="PKY63_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPF01358.1"
                     /translation="MRITALLFIIILFSHSAASQNQNAEQWADSVIRTMTLREKIGQL
                     MMIAVYSNQNEKYAASIDDTIATYQPGAIVFFQGAPQKEVILMNRWQKKAKIPMLVAM
                     DAEWGPAMRLDSIMPLPRLMALAATNDTTLIYRYGQAIGKQCKRLGIHINFAPVVDVN
                     NNPLNPVINYRSFGENQHKVALYASVFCRGMQSQGVMAVAKHFPGHGNTSQDSHKTLP
                     DVTDNINVIDSVHLVPFAKMIDAGIWGIMVAHVFVPALDTTSNIPTTLNQTVVTGLLK
                     NEMGYKGVVITDALGMQGVLKHHKPGQIEVQALIAGNDVLLMPEQIKTAIDSIVAAVD
                     IGRIDSVRIDESVRKILITKYNERLTRYIDIDEKNITDDINAADWYELKRDVFNNNAT
                     LVFNHGIDIPLRNIHKRIAVISFRADRENIFHEELMRHVCADAYFVPENATEKDIEAI
                     TDTIKNYDLAIVAVQNMAYWNKNNYGFNSFVMPAVETICRQTQTILCLFGNPYAYHKW
                     SADVEPKALIVTYERDTLAEIACAQSLFGIQNFKGKLPVSISAKYPENTGITTETGND
                     RLMEMTPAKLGLGNVLLDSVDAIIKKAIDEKVFPGCQVLAAVNGKVFYNKAFGYHDYT
                     KKTPVKRTDIYDLASVSKIFGTTLAIMKLYEEKRFRLDDLLQQHLPLAKNTAAGQLRI
                     DRLLTHSSGLPAWLPLYMATVKNGKPDTNYFRTKADSLFGIRVSDSLFMRCDYIDTML
                     NIILHSKINPSHHYKYSDLGFMLLKEMAEHLSSDSLNRYVDENFYQPLGLSWMTYNPL
                     TRFDKSRIPPTEEDTSFRDVKVHGYVHDQAAAMLGGVSGHAGVFGNAWDAAVILQMLI
                     EKGHYAGTKYFEASTVEKFTSTYFDKNRRGLGFDKPSGIHNGNVCDEASPESFGHSGF
                     TGTFVWADPRNGLIYVFLSNRTYPDAENKKIVEQNIRTRIHSLFYQTIDKP"
     gene            3987..6095
                     /locus_tag="PKY63_11885"
     CDS             3987..6095
                     /locus_tag="PKY63_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01359.1"
                     /translation="MSDKKQKTNKLQKSVLQKPVVVKNNANTGKKKWLPYLVLALMTI
                     FVYSLSFNTEFIYNWDDAGYVTGNAYIKSLNFESIQNIFSEIYYMSNYHPLTTLSYAI
                     DWAIAGDNPVWYHVINLLFHLLNTMLVFVVIKRFFHVKSDWVPFFVAMIFAVHPMHVE
                     SVAWISERKDVMYTFFFLLALLKYHDYKNTTNNKWKHYLLLILFFLMSLMSKSAAVVF
                     PVALFAFDWYERRKLNLMLIAEKIPMLALSLIFGIIALKSQKEAMQDLAPLLSLTERL
                     QIVNHSFLTYIYKFFLPVNLSGFYPYPIKDNNALPDIYSIAPLITAGLLGVFIWLGRK
                     SRELIFGVLFFIINLALVLQLKPVGGAVLAERYTYVPYIGLALPFAFWLGQKADKNKL
                     LQGIILIIGITFSIVSFNRVPVWKSGDALFSNVLEQFPRNPYAWDNRGGLYWDIYASK
                     KFKDNPAKKKIFTDKAYHDYTMSLQLDASYIQPWSHRGILLFNTGRYEEALNDFNVAL
                     KLKPDYIDALLGRANTLSTLQRYEEAIPDYNAYLQQHPDSAQAYVWRATAWHHLQQYD
                     KALSDCEAAIQLNPKNAEAWYWKGLCFYNTGKNEEAISGFAKAIELKPDYQEVFVWQG
                     MAYANLKQYNKAIEFYSKAIQLKPGDAVALVNRSVAYYNLNDFAHALADLDTAGKLGY
                     PLNKDYYLAVKNGDKRFQDK"
     gene            complement(6155..>6560)
                     /locus_tag="PKY63_11890"
     CDS             complement(6155..>6560)
                     /locus_tag="PKY63_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01360.1"
                     /translation="YLFWNLEPEGKQKTKLQLSRRDKILVARKRTPARAVRYGMVYSM
                     RHPESSENDCPDISIVSVSFQLSKSCAYLFWNLEPEGKQKTKLQLPRRDKILVEQKKT
                     PARAVRYGMVYSMRHPESSKIYEPMACICRIL"
BASE COUNT         1998 a         1392 c         1248 g         1922 t
ORIGIN      
        1 tgatagaaat actgcagctt ttcatcctca tcactaccat tgtaacatgg ttcgagcttt
       61 tcattaatgg ctatatcgat agtctgaata tatttattta atcgcacgac catatattgt
      121 ttttagatca ttctcttaaa agctttcttg cattttcatg agcatccgtc gatattaaat
      181 actcatcaaa tccaataaat aaataataaa atagctgggc gttcatttct ctccaattac
      241 cattaaaata aaaaggtcct tccggtgaaa aataaattgc aattttttta aaaaatattt
      301 tatttctaaa aatcagatca actaatattc tgggcgatag gcaacttgtg tcttgagtta
      361 atggataatt ctccaattgg cttttgaaag cgttaaaatc aaatatttta ctacttagta
      421 attcaacatt atatctcaca acgaatattt cgtgtctttt tttaatatta tcaatggaaa
      481 tggggattct atacgatttg ttttttggaa ctaagtacat ctctaccctt tttaaagaat
      541 agtcggagcc atatagaacc attgcatttg caataaatac ccaaaaaaac aaaaaggttt
      601 ttttcatgat tgtactcctt attcttctgt gtcaaaaaca ttgtctgctt taaacaaaat
      661 aataacatca agataatctg ttctttttgt ttcgctgttt cctttgtaat atgttctcaa
      721 agcgttaatc acttctcttt ctttattccc atgtgcataa ttttcctctt gattagaatt
      781 tttatgttcg cggaattcaa gttacaaatg caactttttg tttttcaaaa ttaataataa
      841 ttactgatgt ggagtcaaat atcctaaacg ctttctggga cggtgattta gcttgtattg
      901 aatttagaat atgtcttcat cagcaatgtc ctaaatttca ttcaaatggt aaatgccggc
      961 aaaattattt atgctataat aaaattctcg ttggtaatac tcccctgaaa tgaaaaataa
     1021 tcttgtaagt ctcgcaaaat catgttttct ttgtaataca atttttttca gataaagcta
     1081 tgcgtatcac ggccttactt ttcatcatta ttttattcag tcactcagct gcatctcaaa
     1141 atcaaaacgc tgagcaatgg gctgattctg tcattcggac aatgactctg cgcgaaaaaa
     1201 tcggtcagct gatgatgatt gctgtgtatt ccaaccaaaa tgaaaaatat gctgcatcaa
     1261 tcgatgacac cattgcaact tatcaacccg gagccattgt gttttttcag ggagcgcctc
     1321 aaaaagaagt aatactgatg aatcgctggc agaaaaaagc aaaaattccg atgcttgttg
     1381 ctatggatgc cgaatggggt cctgccatgc gactcgacag tatcatgcct ttaccccgtc
     1441 tgatggctct ggctgccacc aacgacacaa cactcattta ccgctacggg caagccatcg
     1501 gtaaacaatg caaacgcctc ggcattcata tcaattttgc cccggtggtc gatgtaaaca
     1561 acaatcccct gaacccggtc atcaattatc ggtcgtttgg agaaaaccag cataaagtag
     1621 cattgtatgc atcggtattt tgtcgcggaa tgcagtcgca aggtgtaatg gccgttgcca
     1681 aacattttcc cggccatggc aatacatcac aggattcaca caaaacacta cccgatgtaa
     1741 ccgataatat caacgtcata gattctgttc atctcgtgcc ttttgccaaa atgatagatg
     1801 ccggaatatg gggaattatg gtggctcacg tttttgtgcc ggcactcgac accaccagca
     1861 acattcctac aacactgaat caaacagtag ttacaggact tttgaaaaat gaaatgggct
     1921 acaaaggtgt tgtaattacc gatgcgctgg gcatgcaggg agtattgaaa caccataaac
     1981 ccggtcagat tgaagtgcag gcactgatag ccggaaacga tgtattactt atgccggagc
     2041 agataaaaac agccatcgac tctattgtgg cagcagttga catcggccgc atcgactctg
     2101 tcagaattga tgaaagcgtt agaaaaattt taatcacaaa atacaatgaa agactcaccc
     2161 gttatattga tattgatgaa aaaaatatta ctgatgacat caatgcagcg gactggtatg
     2221 aattgaaaag agatgttttc aacaataatg caacactggt ttttaatcat ggaatagata
     2281 ttccgcttcg caatatccat aaaagaattg cggtgatttc gtttcgtgcc gaccgcgaaa
     2341 atatctttca cgaagaactg atgcgacatg tttgcgccga cgcatatttt gttccggaaa
     2401 atgccacaga aaaagacatt gaagcaatta cagatacaat aaaaaattat gatctggcca
     2461 ttgttgcggt gcaaaatatg gcttattgga acaaaaacaa ctatggcttc aattcattcg
     2521 tcatgccggc agtagaaacc atctgccgtc aaacacaaac cattctgtgc cttttcggaa
     2581 atccttatgc ctatcataaa tggtcggccg atgtagaacc aaaagcactt attgttacat
     2641 acgaaagaga tacactggct gaaattgcct gcgcccaaag tctttttgga attcagaatt
     2701 tcaagggaaa actccctgtt tcaatatcag caaaatatcc ggaaaatacc ggcattacaa
     2761 cagaaacagg caacgaccgt ttgatggaaa tgacacctgc gaaactggga ctcggcaacg
     2821 ttctgctcga ttcggtagat gctattatca aaaaggccat tgatgaaaaa gtttttcccg
     2881 gctgtcaggt tttggctgca gtgaacggaa aagtatttta caacaaagca tttggttatc
     2941 acgattacac aaaaaaaaca ccggtaaaac gaaccgatat ctatgatctg gcctcagttt
     3001 caaaaatatt cggaacaaca ctggccatca tgaaattata cgaagaaaaa cgtttccggc
     3061 ttgatgattt gctgcaacaa catctgccct tagcaaaaaa cacagcggca ggacagttgc
     3121 gtatagaccg cttgctaact cattcttcag gacttccggc ttggcttcct ctgtatatgg
     3181 caactgtaaa aaacggaaaa cccgatacaa actacttcag aacaaaagcc gattcacttt
     3241 ttggaattcg tgtaagcgac tcacttttca tgcggtgcga ttacatcgac acgatgctga
     3301 acatcattct gcattcaaaa ataaatcctt cgcatcatta caaatacagc gaccttggat
     3361 ttatgttgct gaaagaaatg gcagagcatc tgagctccga ttcgctcaat agatatgttg
     3421 atgaaaattt ttatcaaccc ctggggttgt catggatgac atacaatccc ttgactcgat
     3481 tcgataaaag ccgcatcccg cctacagaag aagacacttc attcagagat gtaaaagttc
     3541 atggctatgt gcatgatcag gcagcagcca tgctgggcgg tgtttcggga catgcaggtg
     3601 ttttcggcaa tgcctgggat gccgctgtca ttctgcaaat gctgattgaa aaaggtcatt
     3661 acgccggtac aaaatatttt gaagcatcaa cagttgaaaa attcacttca acctattttg
     3721 ataaaaacag acgaggtctt ggtttcgaca aaccatcagg cattcacaat ggcaatgtct
     3781 gcgatgaagc ttcgcctgaa agctttggtc acagcgggtt tacaggaact tttgtgtggg
     3841 ccgatccgcg taacggactc atatatgttt ttttgtcgaa ccgcacatat cctgacgcag
     3901 aaaacaaaaa aattgttgag cagaatatcc gcacaaggat tcattctttg ttttatcaga
     3961 ccattgataa accttaattg ctttctatga gcgataaaaa acaaaaaaca aacaagcttc
     4021 agaaaagtgt tctacaaaaa ccggtggttg taaaaaacaa tgccaatacc ggaaaaaaga
     4081 aatggctccc ctacctcgtt ctggcactca tgaccatttt tgtgtattca ctttctttca
     4141 acactgagtt tatctataat tgggacgatg caggttatgt aaccggaaat gcctacatca
     4201 aatccctgaa tttcgaaagc atacagaata ttttcagcga aatttattac atgagcaact
     4261 atcacccgct cacaaccctc tcctacgcta tcgattgggc tattgccggt gacaatcccg
     4321 tttggtatca cgtaataaac ctgctattcc atttgttgaa taccatgctg gtttttgttg
     4381 tgataaaaag attttttcat gtaaagtcag actgggtgcc gtttttcgta gctatgattt
     4441 ttgcggtcca cccgatgcat gtggaaagtg ttgcatggat ttcggaacgc aaagatgtaa
     4501 tgtacacttt cttctttctg ctcgcattgc tgaaatatca tgattacaaa aacacaacaa
     4561 acaacaaatg gaagcactac ctgctgttga ttttattttt cctcatgtcg ctcatgtcga
     4621 aatcagcagc ggttgtcttt cctgttgcgc tattcgcatt cgactggtat gagcgccgca
     4681 aactaaatct gatgctaatt gcggaaaaaa tcccaatgct cgctttatca ttgatttttg
     4741 gcattatcgc tttgaaaagt cagaaggaag ccatgcagga cctggctccg ctgctttcac
     4801 tcaccgaacg cctgcaaata gtcaaccatt catttctgac atacatttat aaatttttcc
     4861 tgcccgtcaa tttatccgga ttttatccct atccgattaa agacaacaat gcattaccag
     4921 acatttacag cattgcacca ttgattacag caggattact tggtgtgttt atctggcttg
     4981 gacgaaaaag ccgcgaactt atttttggtg ttcttttctt tataatcaac ctcgcattgg
     5041 tattacaact aaaaccggtt ggtggtgcgg ttctcgcaga gcgatatacc tatgttccct
     5101 atatagggct tgctcttcct tttgcattct ggctcggaca aaaagctgat aaaaacaagc
     5161 ttttacaagg aataatcctt attatcggaa taacattttc aattgtttca ttcaatcgtg
     5221 tgccggtctg gaaaagtggc gatgcattgt tcagcaatgt gctggagcag tttccgcgca
     5281 atccgtatgc atgggacaat cgcggcggat tgtattggga tatctatgct tcgaaaaaat
     5341 ttaaggataa tccggcaaaa aagaaaatat ttaccgacaa ggcctatcac gattacacca
     5401 tgtcgctcca gctcgatgcc agctatatac agccatggtc gcaccgcgga attctgctgt
     5461 tcaataccgg ccgctacgaa gaagctctga atgatttcaa tgttgcatta aagctgaaac
     5521 cagattatat tgatgcttta cttgggcggg caaatacttt gagtacactg caaagatacg
     5581 aagaagccat tcctgattac aatgcttatt tgcagcaaca tcccgactcg gcccaggcat
     5641 acgtgtggcg cgccaccgca tggcatcatc tgcagcaata cgataaagct ctgtctgact
     5701 gcgaagcagc cattcaatta aatccaaaaa atgctgaagc atggtattgg aaaggattgt
     5761 gtttttacaa taccggaaaa aacgaagaag ccatttcggg ttttgcaaaa gccatcgaac
     5821 ttaaacctga ttatcaggaa gtgttcgtgt ggcagggaat ggcctatgcc aatctgaaac
     5881 aatacaataa ggcaattgag ttttacagca aagcaatcca actcaaaccc ggcgatgccg
     5941 ttgcacttgt aaaccgctca gtggcctatt acaatctaaa cgattttgca cacgccctgg
     6001 ccgatctcga tacagccggc aaactcggct atccgctcaa taaggattat tatttagcgg
     6061 tgaaaaacgg tgataaaaga tttcaggata aataatttga ttctctgtaa taatttcaga
     6121 aaaaaagttc agaattctca tagtacattt catttcacaa aatccggcag atgcatgcca
     6181 tcggttcata tattttggaa gactccggat gtctcattga ataaaccatt ccgtacctga
     6241 cggcacgggc tggcgttttt ttttgttcta ccaatatttt gtccctacgg ggcaattgca
     6301 gcttagtctt ctgtttccct tcgggctcca aattccagaa caaatacgcg cagctcttgc
     6361 taagctgaaa tgacaccgaa acgattgata tatcggggca atcattttcg gaagactccg
     6421 gatgtctcat tgaataaacc attccgtacc tgacggcacg ggccggcgtt ctttttcgtg
     6481 ctaccaatat tttgtcccta cgggacaatt gcagcttagt cttctgtttc ccttcgggct
     6541 ccaaattcca gaacaaatac
//
LOCUS       DAOPKE010000133         6544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12545_length_6544_cov_10.868084, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000133 DAOPKE010000000
VERSION     DAOPKE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_6544_cov_10.868084"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2147)
                     /locus_tag="PKY63_11895"
     CDS             complement(<1..2147)
                     /locus_tag="PKY63_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPF01361.1"
                     /translation="MERILQQLSSGFHIPLQNPVLIFSLILAIILLAPVLLRKLNIPS
                     IIGLIISGAVIGPFGLNILEKNSAVELFSTIGLLYIMFIAGLELDFSEFRTNKFKNFL
                     FGLFTFFTPIIVGFPIMFYVLDYDLLPAMLIASMFSTRTLVAYPIVSKLGISKNKAVA
                     VSVGGTIIADTGVLIALTMLLGAEHGGTGKDFWIQTILSIFLFAIIMFAAVPRIAKWF
                     FNKLESEKYSHYIFVLAVVFFSAFLAEVAGMEPIIGAFAAGLSLNKLIPATSSLMNRI
                     EFIGNSLFIPFFLISVGMLVNVRLLFEGYEALLIAAILAVVSLSGKWMAALATQIIFG
                     FSRAQRSLVFGLSSAHAAAVLAISLVGNKAGLIDEVILNGIIILILITCIFSSFVTER
                     AARKVVLEMDNAPITPENGKNVHKEHILIPVTKIRSMEKMIDFVALLKDHKSLHPITM
                     LAIVPNDEDSEGKIIKAQNDLEKCVKHASATETKVNIAVTIDQHFLSGVARKSREIMA
                     ETIVLPWPEEGDLIDKKSEFRLDNLLEQINKSLFICHLNKPASAYKRIIVVAPPYTEK
                     ESDFGFLMGKIIKLAGELKTEMVFFSNGVTRGAIANALIVLKNQVSNELRPFDNWDDF
                     LILSRFIREDDIMVVIGARKGSFSYNQQLDSIPAKLEKYFSRNTRIIFYPKQISSRSI
                     IDSYGDITHEPLSIGLEAVQKIGKEIGNIFKKE"
     gene            2629..3441
                     /locus_tag="PKY63_11900"
     CDS             2629..3441
                     /locus_tag="PKY63_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HPF01362.1"
                     /translation="MNPFFANNLKYLRKRRNLTQDELAIALDMKRSTLSGYENQVAEP
                     SLDDIVRFASFFHISIDSLLTTDFSIMSEYQIHQMELGFDPYVRGSNLRVLATTVDSG
                     NNENIELVEEKAKAGYKSGFADPEYIKVLPTFSLPFLSASKKYRTFQISGDSMLPIPD
                     KAFVTGEFIQNWNLIRDRQAYIVLTLDDGIVFKVVENRIKSEGKLVMYSLNPMYQPYE
                     LNVAEIKEVWKFVHYISPELPDPVLPRNELQTTVAEMKRDLDSIKRQLGSKR"
     gene            3455..4150
                     /locus_tag="PKY63_11905"
     CDS             3455..4150
                     /locus_tag="PKY63_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPF01363.1"
                     /translation="MIIEQLFSLLNEQKPFVSYELHRGKIFSAIQDIEGGIGIAASPD
                     FPEDKDQADDYLNKVIMQCRINARINQPNRHFSQVDFVDYAGLSGQEKIVMAGFIRPV
                     YEQLLDKGISCHVFDLKKEAPELLPIGEMDDYLRDADVLISTGTVFSNGSIDNMSAKL
                     KSNARLYVIGPSAPLAPVLFDWIPALQGIFGSVVKTKEILPKINSGAGTRQLHNELQK
                     VALLRNSTSQDKS"
     gene            complement(4128..4547)
                     /locus_tag="PKY63_11910"
     CDS             complement(4128..4547)
                     /locus_tag="PKY63_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPF01364.1"
                     /translation="MRTIFVIIFLSAVFISQAQINPTKDTNKYSDPQNMDVVYTSEAK
                     CTMGEDQMYKTIYSGITYSEEAKKANIQDEVLISFDVNFDNKLQDFTIVHGVGYGIDE
                     QIIAVIKTLKFEAAVMNGIKVRQNVMLTIPIRTYPEM"
     gene            4607..5965
                     /locus_tag="PKY63_11915"
     CDS             4607..5965
                     /locus_tag="PKY63_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPF01365.1"
                     /translation="MKLKNVTYYQTIVVLFVCFFFLSGGIGAQNYNDSLLDGSWAGNA
                     DKVQRAILNKADLNYTLLDGATALHYACGGNHLAVVKLLVSGGAAINLPDKKGRIPLH
                     VVAENGNDSIGEFLIVNGALLYAKNSEGYTPLMVAVSRGYFIFSDLCLFYGSDVNIRP
                     DDSSSVCHLCVHNGNPHVLQLLIENGADINLCDADGKTPLAWAVLYNDTACTGILLNA
                     GATLQTACSDLSVNTLLSEAILKGCDATLPYLLKNPSVRGKTDLRRLRDDVIRIDHRQ
                     MRLAFRNDSIPLSWRPVFQGMLFKPELFVNFRDHFTGFSMGTMEMKSKIGLEMGVATR
                     LWKKRVLFDYEPTGDLLQLQEKRGFVYFGQYKAIRVLQKSFSGLQLEPGIQQTYSWAY
                     FDGMSTRPWRGWTVSPAFDVVWFGRNWNVSLGARFYDFNNSLSSFYFSLSGGWVIPFK
                     NR"
     gene            5967..6380
                     /locus_tag="PKY63_11920"
     CDS             5967..6380
                     /locus_tag="PKY63_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01366.1"
                     /translation="MKQFRYLIMVLPLLFVLSCVDDDYDDCDMQDCVSIYPETGTLTV
                     KVTLNSMNSLVPIRIYAGHYDDGELIREDTLYSERQNYVYEPGIYYSVAATYKTESGV
                     ITVVDGDKIEVITNTECDTTCYTVNDATVNCKLHY"
BASE COUNT         1846 a         1329 c         1419 g         1950 t
ORIGIN      
        1 tcttctttct taaaaatatt cccaatctct ttcccaatct tctgaacagc ttccaaccca
       61 atgctgagcg gctcgtgcgt gatatcacca tagctgtcga tgatgctgcg gctgctgatt
      121 tgtttgggat aaaaaataat ccttgtgttg cgtgagaaat atttttcaag cttcgcagga
      181 atgctgtcca attgctgatt gtatgagaag gatcctttgc gggcgccaat aacaaccata
      241 atatcgtctt cgcggatgaa acgcgaaagg atcagaaagt catcccagtt atcgaatggt
      301 ctgagttcgt ttgagacctg attttttaat acaattaagg catttgcaat ggctcctcgt
      361 gtaacaccat ttgagaaaaa aaccatttcg gtttttaatt cgcctgcaag cttaatgatt
      421 tttcccatga gaaagccaaa atcgctttcc ttttcagtgt atggcggcgc aacaacaatt
      481 attcgtttat aagcagaagc aggtttattg agatgacaga tgaaaagcga tttatttatc
      541 tgttcaagca ggttgtcaag tctgaattca gattttttat caatcaaatc gccttcctca
      601 ggccagggca gaacaatagt ttcagccatt atttcacgcg atttgcgtgc aaccccgctc
      661 agaaagtgct gatcgatagt gacggcaatg ttcacctttg tttcagtagc ggatgcatgc
      721 ttgacacatt tttccagatc attctgggct ttaatgattt tgccttctga atcttcatca
      781 ttgggtacaa tggccagcat ggtgattggg tgcagcgatt tgtggtcttt gagcagagca
      841 acgaaatcaa tcattttttc catcgatctg atcttggtca ccggaatgag aatgtgttcc
      901 ttgtgaacat ttttcccgtt ttcgggagta attggtgcat tgtccatttc aagaaccact
      961 tttctcgcag cccgctctgt tacaaatgat gagaatatac aggtgataag aatcagaatg
     1021 atgatcccgt tcaatatcac ttcatcaatc agccctgctt tgtttccaac gagtgatatg
     1081 gcaagcactg cagcggcatg tgcactgcta aggccaaata caagactgcg ttgtgcccgg
     1141 ctgaatccaa aaatgatctg ggtcgcaagt gctgccatcc attttccgct gagcgaaaca
     1201 acggccagta tagctgcaat gagcagtgcc tcatatcctt caaacaatag tctgacatta
     1261 accagcatcc ctactgatat cagaaaaaac ggaatgaata gtgaattgcc aataaattca
     1321 atcctgttca tcagacttga tgtggccgga atcagtttgt taagcgataa tcctgctgca
     1381 aatgcaccga tgatgggctc cattccggct acttctgcca gaaatgccga aaagaatact
     1441 actgccagta caaatatgta atgtgagtat ttttcacttt ccagtttgtt gaaaaaccat
     1501 ttggctatgc ggggaacggc tgcaaacata atgatggcaa acagaaaaat ggataaaatg
     1561 gtctgtatcc agaaatcctt gcctgttcct ccgtgttctg cgccaagtag catagtgagc
     1621 gctatcagaa ctccggtgtc cgcaataatt gttccgccaa ccgataccgc aacggctttg
     1681 tttttcgaaa tccccaactt gcttacaatt ggataggcaa ccagtgtccg ggtcgaaaac
     1741 atgctcgcaa tcagcatggc aggtagtaag tcataatcca gaacataaaa cattattgga
     1801 aaaccgacaa taatcggtgt gaaaaaagta aataaaccaa ataaaaagtt cttgaattta
     1861 tttgtcctga attcactgaa atcgagctcc aatcctgcaa taaacatgat atacaataag
     1921 ccgatggtcg agaacaattc aacagccgaa ttcttttcaa ggatgtttaa tccgaaaggg
     1981 ccaatgacag ctccggaaat gattagtcca atgatgctcg gtatgtttag ttttcgtagc
     2041 aataccggcg ccagcagaat aatggccaga atcaacgaaa atatcagcac cggattctgg
     2101 agcggaatgt gaaaacctga agatagctgc tgtaaaattc tttccatgtt cagatgtccg
     2161 gcggtgagtt attgcattta tacgcaaatt atgattgaaa gtatacattt aaatacctga
     2221 gtttagaaaa atttcaatat tacatggatt tcatctctgg tttttcttgg tttactatgc
     2281 tggatgcggc agtgattatc attgagctca tcctgagacc acaagagatg tgcacatctc
     2341 taccaaaatg aaaacttcag tttaatttgc tgtcatcctg agacccgcgt gcagtcctcc
     2401 tgacattacg cgacaggcac cagcaaaacg cgttttgcaa cctaaataaa aaatgacaaa
     2461 aaaatactgt cacgctgagc cctgcctgct gtcacactga gccctgcgtc ccgcaggata
     2521 aactccgtcg aagtgtcaac agcaggttaa actccgtcga agcgtcagca acaaaaataa
     2581 tttttgacta ttagaaaata atcactatat ttgccgaaaa tcacatcaat gaatccattt
     2641 tttgccaaca atctcaagta tttgcgcaag cgacgtaacc tgactcagga tgaactggcc
     2701 attgcacttg atatgaaaag atcgacgctg agtggttacg aaaatcaagt ggcagagccg
     2761 agtcttgacg atattgtccg ctttgcctcc ttttttcata tctctatcga cagcttgctg
     2821 acaacagatt tttctattat gagtgaatat cagattcatc agatggaact tggatttgac
     2881 ccctatgtgc gcggcagcaa tctgcgcgta ttggctacca ctgttgattc aggcaataac
     2941 gaaaatattg aattggtcga ggaaaaggcc aaagccggct ataaatcagg gtttgccgac
     3001 cccgaataca ttaaagtttt gcctacattt tcgctcccgt ttctgagcgc cagcaaaaaa
     3061 taccgtactt ttcagatcag tggcgacagt atgctgccca ttcctgacaa agcgtttgtt
     3121 accggcgaat tcattcagaa ttggaatctt atccgcgacc gtcaggccta cattgtcctg
     3181 acactcgatg acggcatcgt gttcaaagtc gtcgaaaacc gcataaaatc cgaaggcaag
     3241 ttggtgatgt attcgctcaa tcccatgtat cagccctatg aattgaatgt agctgaaata
     3301 aaagaagtct ggaaatttgt tcattacatc agcccggaac tgccagatcc ggttttacct
     3361 cgcaatgagc ttcagaccac agttgcagaa atgaaacgcg accttgatag tatcaaacgt
     3421 caattaggat caaaacgcta aaaacagaaa taaaatgatt attgaacaac tgttcagtct
     3481 gcttaatgaa caaaagccct ttgtttctta tgagctgcat agagggaaaa ttttttcagc
     3541 tattcaggat atcgaaggtg gaattggtat tgcagcttct cccgattttc ccgaagataa
     3601 agaccaagct gatgattacc tgaataaggt gattatgcaa tgtcgcatca atgcaagaat
     3661 aaatcaacct aacaggcatt tcagtcaggt ggattttgtt gattatgccg ggctttccgg
     3721 acaggaaaaa attgtgatgg cgggcttcat tcgtcctgtt tatgagcagt tgctcgataa
     3781 aggcatttct tgtcatgttt ttgatttgaa aaaagaagcg cccgaattac tgcctatcgg
     3841 cgaaatggat gattatttgc gggatgcaga tgtgttaata agtactggaa ccgttttttc
     3901 aaatggcagc attgataata tgtcggctaa gcttaaaagc aatgcacggc tatatgttat
     3961 cgggccttca gctcctttgg ctccggtgtt gtttgattgg attccggctt tacagggcat
     4021 ttttggcagc gtggtaaaaa caaaagaaat actcccgaaa atcaattcgg gagctggtac
     4081 ccgccaatta cacaatgaat tgcagaaagt agccctgttg cgaaactcta catctcagga
     4141 taagtcctga taggaatggt cagcattaca ttttgacgta ccttgatgcc attcataacg
     4201 gcagcttcga acttcagtgt tttaatgacg gcaataatct gctcatcaat gccgtatcca
     4261 actccgtgta caatggtgaa atcctgaagc ttgttatcga aattcacatc aaaactaatc
     4321 agcacttcgt cttgaatgtt ggcttttttg gcttcttcgc tgtaagtaat tccactgtaa
     4381 atggttttat acatctggtc ttcgcccatg gtgcatttag cttctgatgt ataaaccaca
     4441 tccatatttt gcggatcgga atacttgttt gtgtctttgg tgggattgat ttgcgcctgt
     4501 gaaataaata cggcgcttaa aaaaataatt acaaaaattg ttctcatggt tgggggattt
     4561 tattattttt ggaactataa aacaactact ctgccaattt ttacggatga aacttaaaaa
     4621 cgttacttat tatcagacta ttgttgttct ttttgtgtgt tttttctttc tgtctggggg
     4681 aattggagca caaaactaca atgattcact gctcgatggt tcatgggctg gcaatgccga
     4741 taaggtgcag cgggctatat tgaataaggc cgatctcaat tacaccttgc tggatggtgc
     4801 tacggcactg cattatgcct gcggcggcaa tcatctggct gtcgtaaaat tgctggtctc
     4861 gggaggtgca gccattaatc tgcctgataa aaaagggcga attccattac atgtggttgc
     4921 cgaaaatggg aatgacagca tcggtgaatt tttaattgta aacggagctt tattgtatgc
     4981 aaaaaattcc gagggctata ctcctttgat ggtcgctgtt tcacgcggtt attttatctt
     5041 ttccgatcta tgcctctttt atggaagcga tgtcaatata cgccctgatg attcttcatc
     5101 agtatgccat ttgtgtgtgc ataatggcaa tccgcatgtt ttgcaattgc ttattgaaaa
     5161 tggggccgac attaatcttt gtgatgccga tggaaaaacg cctcttgcct gggcggtgct
     5221 ttacaacgat accgcttgta ccggaatttt gttgaatgcc ggtgccacat tgcagactgc
     5281 ctgctccgac ttaagtgtca acaccctgtt aagtgaggct attctcaaag gatgtgatgc
     5341 tactcttcct tatttgttga aaaatccgtc tgtcagaggg aaaaccgatt tacgccgttt
     5401 gcgcgatgat gttatacgga tcgatcacag acagatgaga ctggctttca gaaacgacag
     5461 tattccactt agctggcgcc ctgtttttca gggaatgctt tttaaacccg aattattcgt
     5521 caacttcagg gatcatttta ctgggtttag tatggggact atggaaatga agtcaaaaat
     5581 cggcttggaa atgggtgtgg ccactcgttt gtggaagaaa agagttctgt tcgattatga
     5641 gccgaccggg gatttgctgc aattgcagga aaagaggggc tttgtttatt ttggtcaata
     5701 caaggccatt cgtgttttgc agaaaagttt ttccggattg cagttggagc cgggtattca
     5761 gcaaacatac agctgggctt atttcgatgg catgtcaacc cgcccctggc gcggatggac
     5821 tgtttctcct gcatttgatg tagtttggtt cggccgcaac tggaatgttt ctctgggtgc
     5881 ccgattctat gatttcaata attctttatc atctttttat ttttcgttga gtggaggatg
     5941 ggtgataccg tttaaaaacc gataaaatga aacaattcag atatttgata atggtactgc
     6001 cattattgtt tgttctttcc tgcgtcgatg acgattacga tgattgtgat atgcaggatt
     6061 gtgtttccat atatccggaa accggaacgc tgactgtgaa agtgacattg aattcgatga
     6121 attctttggt accaatccgt atttatgccg ggcattatga tgacggggaa ttgattcgtg
     6181 aagacacatt atattctgag cggcagaatt atgtgtatga gcccggaatc tattattcag
     6241 ttgccgcaac ttataagacc gaaagcggtg ttatcacagt ggtcgatggt gataaaatcg
     6301 aagtgattac caatacagaa tgcgacacaa cctgctatac agtcaatgat gccactgtca
     6361 actgcaagct gcattattga aagattgcaa gtggttttcg agtgacaaat attgactttg
     6421 caaattgcta tgatttgatt ctcaacataa atctatgaca ttcggggtat tgcctgactc
     6481 aaagatgagg aataatacca tcgtgaaata tattttattc caatgtgtct aagtgcagat
     6541 tgat
//
LOCUS       DAOPKE010000134         6518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12599_length_6518_cov_11.543091, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000134 DAOPKE010000000
VERSION     DAOPKE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12599_length_6518_cov_11.543091"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PKY63_11925"
     CDS             <1..767
                     /locus_tag="PKY63_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF01367.1"
                     /translation="SWVPSGPNIEPQRADQFSVGYFDRLLKDGFLFSAEAYYKLMRNQ
                     LDYVSHPNLLLNPLLEAEVRNGKINAYGVELMARKNSGALTGWISYTYSRSLVNTPEL
                     NEGRTYPAMWDRPHNISVHVSWTNGHRWKLTANWIYLTGMPVTTPVGYYTYMNHLVPV
                     YDKIHNDRLPDYHRLDISVTLALAKASRKWQHYLEFGIYNLYGRHNPIAITFNKIVDD
                     NGNFVLPSDFSDPALINPVTTAVAGFIPSLTYRFRL"
     gene            764..>1580
                     /locus_tag="PKY63_11930"
     CDS             764..>1580
                     /locus_tag="PKY63_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPF01368.1"
                     /translation="MKNLFLIILSVLIFASCEREAEWPTDQGTLPDIVVEAMLTNEIK
                     HHEVILSLPVSSANENPVMVSNALIDIINNSIVYSFSEDPANPGHYFSDSAFAGIPGQ
                     TYELYFKSSAGRVWAATDKMPSANIFSPLSITLINTEDSLYKISFVCQNYDPSEYAMY
                     EISLDWSGVSGYDTVENKAVLYYYTLGSLDMGEVLQTDLESVIFPKGTLIKERRYSIS
                     ESYAKWLREILIETQWNNGIIVSQPGNANSNIQSFSDSRFVAAGFFSACSVVEW"
     assembly_gap    1581..1680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1701..5051
                     /locus_tag="PKY63_11935"
     CDS             1701..5051
                     /locus_tag="PKY63_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF01369.1"
                     /translation="MKNLFILLSFSFYLLSSATAQENRPHDAYWAEEQGYFSNLHPTD
                     YGIVASSNNCNEIYLINNGELKTLVSAPGCGRYMQLSPDGKTIGFKYISENGMQSPTL
                     FDITTGKTTFLAEPSRLCGQPSFSANGDIVISDTTGFIVYRKNGISEKFATGTYANYT
                     AVSPDGIFVVYNDTNDQLQMFNTQTKEVVAISPEGCALPEFSSSGKYISFVSSLQRIS
                     IYSTEQNRIVTTLYGTATEWHPSQDMLAYNFTESENYTITRAEIKMYNAETGTESLIT
                     GGSENLFFDPAFDKSGNIYFHNQIDLEIFSFQNQGAHCIYSHNGKLPMQFFEMQKSTL
                     SIITVPGTVPYVHQVYDTPTWHSGYWSCAPTTSAMAFGYYNRLPPWPTTVNHGMSWDP
                     HVNNYGSYVADRYRFNEWFYEEIALDQADQTSYGGYGYMWTGSYGPNSRMEDYITQHY
                     ITSNQYWTTSCTWAQTVSEIDNGYVHPICNYLTASGHLTLCIGYYDTQHSLLFNDPYG
                     DRNDATYCDYDGAGVSYDWPTYNNGLANLGGSYSYVAWTVAARTTEPVYNDTIIDNNY
                     YNHGFYVNNSTLGSTQRYFRDYNVGYNGHTWYTIGMTDPDICFTTWTPNISDTAKFIV
                     SAFIPPKGSSTTNAIYQINRLGTDTAIHINQGANKNTWVQLGTFIMCPGEATVRLGDY
                     TGTDGDTIAFDAIKWTRYPNPVAQFSADQTTICQGENVVLTSTSQYSESLIWSCSGGT
                     LTNQTGNQATFSFNTPGTFSVQLITVNFNGRDTLTYNSYINVQPNAVAQFTASADTVD
                     MSSPFVTFTNTSSGADSYVWNLGDGTNTNITNPSHTYSSPGTYIVSLEAYSSSCADDT
                     TILTIVVVNASAVAQFSADQTTICEGDTVVFSSASQNATSLTWNYPGATLINQTGNQC
                     TVVYNNAGTYNAGLIAVSSYGNDTLEYLNYIVVSPNAEAQFIASNDTVPVSNPLIVFT
                     NQSQNANSYLWHFGDGSTSNDVNPYHTYAATFASYNVILEAISAGCADDSATLTIFVI
                     DPTGMNENEKNVFHFYPNPVEDIIYIQSEIADNAVFKIYDTSGQLVMEGDFKNSKSII
                     QVSKLSKGVYSLEILDTNRNFSCFFVKK"
     gene            5048..5827
                     /locus_tag="PKY63_11940"
     CDS             5048..5827
                     /locus_tag="PKY63_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPF01370.1"
                     /translation="MMLIDTHTHLYLPQFDEDLATVAQRAREAGVKKLLIPDIDKDHT
                     EKVKQIVEQFPNLMYGMTGIHPTSVNADYKDQIAHFEKEIENGFPWIAVGEIGTDLYW
                     DKTYFKEQEIVFRHMMETAARLHLPVSIHQRNSMKETLNIMSEFNGKVNGVLHCFSGE
                     KDDAKVAIDLGYKLGISGVITFKNSNLSDIVTYTGLEHLVIETDAPFLAPMPFRGKRN
                     EPAFLKYVVTLLSQTLGVDEAAIAEATTRNALQVFQKIQMS"
     gene            5824..>6518
                     /locus_tag="PKY63_11945"
     CDS             5824..>6518
                     /locus_tag="PKY63_11945"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HPF01371.1"
                     /translation="MNSKKSVLLIYTGGTIGMIRRESDGSLIPLELNNMYHYLPVLES
                     LPVKIDLVSMENIIDSSDMNPEFWKDLVEIIESNYLKYDGFVILHGSDTMAYTASVLS
                     FMLENLNKPVILTGSQLPLGMVRSDGRENLINAIEVAASYDEDTALVPEVAICFENRI
                     FRGNRTSKINAENFNAFISGNYPMLGEIGVKIKFNNAFIAKPSFKNLKVHKELSPRVG
                     ILKLFPGMPDAYTR"
BASE COUNT         1869 a         1507 c         1320 g         1722 t
ORIGIN      
        1 aatcatgggt gccatccggg cccaacattg agccacagcg cgccgatcag ttttctgtcg
       61 gttatttcga tcgtttgctt aaagatggat ttttgttttc agctgaagca tattacaagc
      121 tgatgcgcaa tcagctcgat tatgtgagtc atcccaatct gctgctcaat ccattactgg
      181 aagccgaagt gcgcaatgga aaaataaacg cttacggagt tgaactgatg gcgcgtaaga
      241 acagcggggc actcaccggc tggatcagct acacttattc gcgatcgctg gtgaatacac
      301 cggaactgaa cgaaggccgc acctatcctg ccatgtggga ccgcccgcat aatatttcag
      361 tgcatgtttc atggaccaat ggtcatcggt ggaaactgac tgccaactgg atttatctga
      421 ccggaatgcc tgtcacaacg ccggttggat actataccta catgaaccat ctggtgccgg
      481 tttacgataa aatccacaat gatcgtctgc cagattatca ccgcttggat atttccgtaa
      541 cacttgcact ggccaaagct tcgcgcaaat ggcaacacta tctcgaattc ggtatttata
      601 atctttacgg ccgtcacaat ccgattgcca tcacatttaa taaaattgtg gacgacaacg
      661 gcaactttgt tctgccatcg gatttcagcg atcctgcttt gatcaatccg gttaccacgg
      721 ctgtagcggg ctttattcct tcattaacgt acaggttcag actatgaaaa atctatttct
      781 gatcatattg tcagtcctga tatttgcttc gtgcgaacgc gaagcagagt ggccaacaga
      841 ccagggaaca ttgccggata ttgttgtgga agccatgctg accaatgaaa taaagcatca
      901 tgaggtgatt ctcagtctgc ctgtttcatc cgctaatgaa aatccagtaa tggtctcaaa
      961 cgcgcttatc gacatcatta acaacagcat cgtttactct ttttcagaag acccggcaaa
     1021 cccgggacac tatttttcag attcggcttt tgcaggtatt cccggccaaa cttacgagct
     1081 gtatttcaaa tcttccgcag ggagagtttg ggctgcaact gataaaatgc cgtcggcaaa
     1141 tatattctcc cccttgtcaa tcactttaat caacaccgaa gatagtcttt ataaaatttc
     1201 attcgtttgt cagaattatg atccatcgga atacgctatg tacgagatat cgctcgactg
     1261 gagcggcgtt tcgggctatg acactgttga aaataaagct gttttatatt actacacact
     1321 cggatcactc gatatgggcg aagtcctgca aaccgatctt gaatctgtaa tatttccgaa
     1381 aggcacactg ataaaggaac gcagatattc tatttctgaa tcatacgcaa aatggctgcg
     1441 tgaaattctg attgaaacac aatggaataa cggaattatt gtttcccagc cgggcaatgc
     1501 aaacagcaac attcaatcat tcagcgattc cagatttgtt gcggctggtt ttttcagcgc
     1561 ctgcagtgtg gtggaatgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 aacaaaaatc tcagggggtt atgaaaaatc tttttatcct tttatctttt tccttttatc
     1741 ttttgtcttc agcaactgca caggaaaacc gtccgcatga cgcatactgg gccgaagagc
     1801 aaggatactt ttccaatctt catcccactg attacggaat tgttgccagt tcaaacaact
     1861 gcaatgaaat ttatctgatc aacaacggcg aactgaaaac gctggtttct gcgcctggat
     1921 gcggccgcta catgcaactg agcccagatg ggaaaacgat cggattcaaa tacatttctg
     1981 aaaacggcat gcagagcccg acactttttg atattacaac cggcaaaaca acttttcttg
     2041 cagagccctc acgattatgc ggccagccct cgttttcagc caatggcgat attgtaatat
     2101 ccgacactac aggtttcatt gtttatagaa aaaatggtat ttctgaaaaa tttgcaacag
     2161 gcacatacgc taattatact gctgtttcgc cagatggaat ttttgttgtg tacaatgaca
     2221 ccaatgatca gcttcagatg ttcaacacgc aaactaaaga ggttgtcgct ataagccccg
     2281 aaggatgcgc gcttccggag ttttcatctt ccggaaaata tatttctttt gtttcttcgt
     2341 tgcagcgtat ttccatttac agcaccgaac aaaaccgcat tgtgacaact ttgtatggca
     2401 ctgcaaccga atggcatcca tcgcaggata tgcttgccta caattttacc gaatcggaaa
     2461 actacaccat tacgcgtgcg gaaataaaaa tgtataatgc tgaaacaggc actgaatcac
     2521 tgattacagg tggttctgaa aatttgtttt tcgatccggc cttcgataaa tcaggaaata
     2581 tatattttca taatcagatt gatcttgaaa ttttttcatt tcaaaaccaa ggtgcacact
     2641 gcatttacag tcataatgga aagctgccta tgcaattttt cgaaatgcaa aaaagtacgc
     2701 tatcaattat cactgttccc ggtactgtcc cttatgtgca tcaggtctat gatacaccta
     2761 catggcattc gggctactgg agctgtgcac cgacaacatc ggccatggca tttggctatt
     2821 acaaccggct gccgccatgg cctactactg tgaatcatgg catgtcatgg gatccgcatg
     2881 taaacaatta cggttcttat gttgccgatc gctatcgctt caatgaatgg ttctacgaag
     2941 aaatcgcgct ggatcaggca gaccagacat cctacggcgg atacggctac atgtggaccg
     3001 gctcttatgg cccaaacagc cgcatggaag attacatcac tcaacattac ataacatcga
     3061 atcagtactg gaccaccagc tgcacctggg ctcagactgt ttcagaaatc gacaacggat
     3121 atgtccatcc tatatgcaat tatctcactg catcaggaca tctcactttg tgcatcggat
     3181 attacgacac gcaacattct ttgttgttta atgatcctta cggagaccgc aacgacgcca
     3241 cctactgtga ttatgacggc gccggcgttt catacgactg gccaacatac aacaatggac
     3301 tggccaatct cggcggctcc tacagctacg tagcgtggac cgttgccgca cgaacaacgg
     3361 agccggtgta caatgacacc atcatcgaca acaattatta caatcacggt ttttatgtga
     3421 acaacagcac gcttggcagc acacagcgct attttcgcga ttacaatgtc ggttacaacg
     3481 gccatacgtg gtacaccatc ggaatgacgg atcctgacat ctgcttcact acatggacac
     3541 caaacatcag tgatactgca aaattcattg tcagtgcctt tattccaccg aagggatcca
     3601 gcactacaaa cgctatttat caaattaacc gtctcggtac tgatactgct atacacatca
     3661 atcagggagc aaataaaaac acctgggttc aacttggaac cttcatcatg tgtccgggag
     3721 aagcaactgt gcggcttggc gattatacag gcaccgacgg agacaccatc gcattcgatg
     3781 caattaagtg gacccgttat ccaaatcccg ttgcacagtt ttcggcagat caaacaacaa
     3841 tctgtcaagg tgaaaatgtt gtgttgactt caacaagtca gtacagtgaa agtctcatct
     3901 ggagctgctc aggaggaaca cttacaaatc aaaccgggaa tcaggccaca ttcagtttta
     3961 atacgcctgg taccttttcg gtacaactaa ttacggtaaa tttcaatggt cgtgatacac
     4021 tcacctataa ttcctatatc aacgtacaac ccaatgctgt tgcgcaattt acagcatctg
     4081 ccgatacagt tgacatgtca tcaccatttg tgactttcac caacacttca tcaggagccg
     4141 attcatatgt atggaacctg ggcgacggga ccaacaccaa cataacaaat ccatctcaca
     4201 catactcttc tcctggaaca tacatcgttt ctttggaagc ttactcatca agttgtgccg
     4261 atgatacaac aatcttaacc attgtagtcg tcaatgcctc tgctgttgcg caattcagcg
     4321 cagaccaaac aacaatctgc gaaggagata ctgttgtatt cagctctgca agccagaatg
     4381 caacttcgct cacatggaat tatcccggtg caacattaat aaatcaaacc ggcaaccaat
     4441 gtactgttgt ttacaataat gccggaacat acaatgcagg gctgattgca gtgagttcat
     4501 acggaaacga cacgcttgag tatttaaact acatagttgt atcacccaat gctgaagcgc
     4561 agttcatagc atcaaacgac accgtgccgg tttcaaatcc gctgattgtt ttcaccaatc
     4621 aatcgcaaaa cgccaattca tatctctggc acttcggcga cggatccacc agcaacgacg
     4681 ttaatccata tcatacctat gcagcaactt ttgcttcata caacgtaata ctggaagcaa
     4741 tttccgccgg ctgtgccgat gattctgcaa cactgaccat ttttgttatt gacccgactg
     4801 gcatgaatga aaacgaaaaa aatgttttcc atttttatcc aaatcccgtt gaagatatca
     4861 tttatattca atcggaaata gctgataatg ccgttttcaa aatatatgac acttcaggcc
     4921 aacttgtgat ggaaggtgat tttaaaaatt caaaatcgat tattcaagta tcaaaacttt
     4981 ctaaaggtgt ttattctctt gaaattttag acaccaatcg gaatttttcg tgtttctttg
     5041 taaaaaaatg atgttgattg acacccatac tcacctctac cttccgcagt ttgatgaaga
     5101 tctggcaact gtcgcacaac gcgcacgcga agccggtgtt aagaaattgc tgattcctga
     5161 tattgacaaa gaccacacgg agaaagttaa acaaattgtt gaacagtttc cgaatctgat
     5221 gtatggcatg accggcattc atccgaccag tgtgaatgcg gattacaagg accagattgc
     5281 gcattttgaa aaggaaattg aaaacggttt tccatggatt gctgtgggcg aaatcggtac
     5341 agatctgtac tgggacaaaa catatttcaa agagcaggaa atcgttttcc gccacatgat
     5401 ggaaaccgct gcccgactgc atttacccgt ttccatacat cagcgtaatt cgatgaagga
     5461 aacgctgaac atcatgtctg aattcaatgg aaaagtgaat ggcgttctgc attgtttcag
     5521 cggagaaaag gacgatgcaa aagtggccat tgatcttgga tacaagcttg gaatcagcgg
     5581 cgtaatcact tttaaaaaca gcaacctgag cgatattgtg acctacacgg gacttgagca
     5641 tcttgtaatt gaaaccgacg cgccttttct ggcacccatg cccttccgcg gaaaaagaaa
     5701 cgagcctgca tttctgaaat atgttgttac tttgttgtcg caaacgctcg gtgttgatga
     5761 agccgccatt gcagaagcca caacccgcaa cgctttgcag gtttttcaaa agatccagat
     5821 gtcatgaaca gtaaaaaatc agtactcctc atatacaccg gcggcaccat tgggatgatc
     5881 cgccgcgaat cagacggatc gctcatcccg cttgagctca acaatatgta tcactatctg
     5941 ccggtactcg aaagtcttcc ggtgaaaata gatctggtga gcatggagaa catcatcgat
     6001 tcatcggata tgaacccgga attttggaaa gacctggtgg aaattattga aagcaattat
     6061 ctcaaatacg atggatttgt cattctgcat ggatcggaca ccatggcata tacagcatcg
     6121 gtactgagtt ttatgctgga gaatttaaac aaaccggtaa ttctgaccgg ctctcaactg
     6181 ccgcttggta tggtccgaag cgatggccgc gaaaatctca tcaatgccat tgaagtggca
     6241 gcatcctacg acgaagatac cgcgctggtt cccgaggtag ccatttgttt cgaaaaccgg
     6301 attttccgag gaaatcgaac atctaaaata aatgcggaaa acttcaacgc ctttatatcc
     6361 ggcaattatc caatgttggg cgaaatcggc gtaaaaataa aattcaataa tgcttttatt
     6421 gcaaagccaa gttttaaaaa tctgaaagta cacaaagagc tttcgccacg ggtaggcatt
     6481 ctaaaactgt ttcccggaat gcccgatgca tacacgcg
//
LOCUS       DAOPKE010000135         6397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_12955_length_6397_cov_15.108325, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000135 DAOPKE010000000
VERSION     DAOPKE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12955_length_6397_cov_15.108325"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1697)
                     /locus_tag="PKY63_11950"
     CDS             complement(402..1697)
                     /locus_tag="PKY63_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF01372.1"
                     /translation="MPISHPRKTPKLLYALDDGSYYDHPYLETVGRSGNNIVRLKPSD
                     FIPLPEGSEIFLLQGHSAIGFNSRKNEMEVLHNKTAISSFIAPAHTQTYLAASKQRKN
                     AVVLPLYAYSAVGFLDGKMWATAVRVDPDIRQDCEKFDQNLVERNVVEVRKKFPHNRL
                     LEHIAHCATVYYCPAARNFFLYRWEGPLPTSPSCNSRCIGCISWQPKSSGITSPQNRI
                     TFIPTPEEIAELAVFHMSHAPDPIMSFGQGCEGEPLQVWETILESIKIIRSKTKDGII
                     NINTNGSNPHAVEKLINAGLDSIRISMNSAQEKLYNLYHNPTNYSFSDLAESCKVARR
                     AGKWVSLNYFVFPGITDTFAETDALCRFLDINNPNMIQWRNFNIDPDLYGNIAKEFTG
                     EVRGVAALIDLIKNRYPNMYNGYFNPGVEIQKKYLSISS"
     gene            complement(1704..2072)
                     /locus_tag="PKY63_11955"
     CDS             complement(1704..2072)
                     /locus_tag="PKY63_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPF01373.1"
                     /translation="MAIQKFEDIIAWQKGQDFAYEIYLKFNDLRDFGFRDKIRRAAVS
                     ISNNIAEGFDRRTDADFARFIHIAIGSCSEVKSMLYLAARLNYIEKEKSQLLLEQANE
                     ISKISRGLVKSLLRSSGPNA"
     gene            complement(2160..2762)
                     /locus_tag="PKY63_11960"
     CDS             complement(2160..2762)
                     /locus_tag="PKY63_11960"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPF01374.1"
                     /translation="MFSGIVESMGEITKVTREGSNIHFTVKSDLTKELKIDQSMSHDG
                     VCLTVVKVDKKNKEYVVTAIDETLNKTCLRNWKKGYQVNLERSMKADGRFDGHIVQGH
                     VDQTATCIKVETFDGSWKYHFEYDPKLNNFTVTKGSISVNGVSLTVVDSKPGKFSVAI
                     IPYTFDNTNFHNFKKGTVVNIEFDVLGKYMQRLMEQYKQA"
     gene            complement(2786..4180)
                     /locus_tag="PKY63_11965"
     CDS             complement(2786..4180)
                     /locus_tag="PKY63_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPF01375.1"
                     /translation="MELFEKQVYVSHRERLLKSGLKGIALFIGNTESPMDYPANGYHF
                     RQDSTFSYFFGLDIPDMVGIIDFDEGKHILFANDVDIEDIIWMGPQPAVKDLAAKVGI
                     VETYPFAELESYLVKVKNAGRAIHFTPPYRAESKILLNTMLGIPFTEMKTKASIDLIK
                     AVVALRAIKEQYEIEELERAAKIGYDMHMIAFCNARPGVVEQEVCGLMEGVAYQYGKG
                     PSFPIILSVRGETLHNHNHSNVMQAGQLLLSDAGAQSNKYYTSDYTRTMPVSGKFSEQ
                     QKMIYQIVLDANNHALSVTKPEVTYQSVHLSVSRVIAEGLKNAGLMKGNVDEAVKAGA
                     HAMFFPHGLGHMMGMNVHDMEDLGENYVGYDDEVSRIDQFGTAYLRMGRRLKAGHVMT
                     DEPGIYFIPELIDQWKAEKKFEEFINYAEVEKFKTFGGIRLEDDILVTEGGSRFIGKR
                     LPITIAEVEDAMKK"
     assembly_gap    4246..4345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4355..5590
                     /gene="pepT"
                     /locus_tag="PKY63_11970"
     CDS             4355..5590
                     /gene="pepT"
                     /locus_tag="PKY63_11970"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPF01376.1"
                     /translation="MKQTLLNRFLRYVALDTQSDPNSDTVPSTNKQLTLANALATELK
                     TFGVTDVSLDSKGYVMASIPSNIDRDITPIGFIAHMDTSPDMPGNDVKPQIIESYDGG
                     DIVLNAEKNIHLSPEEFPDLLRYKGQTLITTDGTTLLGADDKAGIAEIMTAIEYLMEH
                     PVIKHGKICIAFTPDEEIGRGVDHFDVKKFGATFAYTLDGSGPGELEYENFNAASATI
                     KIKGNNIHPGYAKNKMLNASLLANEFISMLPVNERPEFTEHYDGFFHLTGLTSTVEEA
                     EISYIIRDHDKAIFERRKKQMQDIVDYLNGKYKKETFLLDIKDQYYNMREMIEPVFHV
                     VEIAMQAMRNVGVKPDVKPIRGGTDGSRLSYMGLPCPNLFAGGHNFHGKHEFVAVESM
                     VKAVDTILNIIAIVSAKEA"
BASE COUNT         1750 a         1417 c         1345 g         1785 t
ORIGIN      
        1 catgtttgga tacctgtttt tgatcagatc aatcagagcg gccactccgc gcactgtatt
       61 tctgaattgc actgacgtcc ggaggatgtc aaacaataat agcgtccggc gaagccggat
      121 gtaaaaatgc acaaatataa gaccctgaca gggtctaatg cccctgattt aaaaaaatca
      181 ttgcgttaac atcgttttta ttcgacctct ccgaggtcgg ttttttttat tttttacccg
      241 gccttttcac gtaaaacgtg acacagcagg ccgggctatt attgtttgac caccgctgca
      301 ggtcggcgat gttcaaaaaa gcatcagaat gatcaattta ttccattgaa tttgtttaca
      361 caaatatttt agaataaaaa tcagatgtcc acatgaagaa gtcaggaaga aattgataaa
      421 tattttttct gaatctcaac acccggatta aaatatccgt tgtacatgtt tggatacctg
      481 tttttgatca gatcaatcag agcggccact ccgcgcactt caccggtgaa ttctttagca
      541 atatttccat acaaatccgg atcgatgttg aaattgcgcc actgaatcat attcggattg
      601 ttgatatcca gaaaacggca cagcgcatcg gtttcagcaa aagtatctgt aatgccggga
      661 aaaacaaaat aattcagcga aacccacttc cctgctctgc gggccacttt acaactttca
      721 gccagatcgg aaaatgaata attggttgga ttgtgataca ggttatacaa cttttcctgc
      781 gcactgttca tactgatccg aatgctgtcg agccctgcat tgatgagctt ttcaacagca
      841 tgcggattgc taccgttggt attgatatta atgattccgt cttttgtttt tgaacggata
      901 attttaatgg attcaagaat ggtttcccaa acctgcaagg gctcaccttc gcatccctgg
      961 ccgaaactca tgattggatc cggcgcatga gacatgtgaa aaacagccag ctctgcaatt
     1021 tcttcgggtg tcgggataaa agtaattctg ttttgtggtg aagtaatccc cgatgatttt
     1081 ggctgccacg aaatacaacc aatgcatcgc gaattgcagg atggacttgt tggtaaagga
     1141 ccctcccacc gatagagaaa aaaattgcgg gcagccggac aataatatac cgttgcgcaa
     1201 tgagctatgt gctccagaag acgattgtga gggaattttt ttcttacctc aacaacattt
     1261 ctttcaacca ggttctggtc aaacttttcg caatcctgtc ggatatcggg atcgacacgc
     1321 acagcagttg cccacatttt cccatcgagg aaaccaacgg cagagtaggc gtagagcggt
     1381 aatacaaccg cattcttcct ttgcttcgac gctgcaagat aagtttgtgt atgtgccgga
     1441 gcgataaaag agctgattgc cgttttgttg tgcaacacct ccatctcatt tttacggctg
     1501 ttgaagccga tagcagaatg tccctgcaat aaaaatattt cagaaccttc gggcagcgga
     1561 ataaaatcag atggcttcag acgaacaata ttgttgccgc tgcgacctac tgtttcgaga
     1621 tagggatgat cgtaatagct cccgtcatcg agggcgtaga gaagctttgg agtttttcgt
     1681 ggatgggaaa taggcataag gcgtcaggcg ttaggaccgc tacttcttag tagtgatttt
     1741 actagtccac gtgaaatctt tgatatttca ttggcttgtt caagaagtag ctgagatttt
     1801 tctttttcaa tataatttag tcttgcagca agatataaca ttgattttac ctcactgcaa
     1861 gagccaattg ctatatgtat gaatctggcg aaatcagcat cagttcttcg atcaaagccc
     1921 tctgcaatat tgttggaaat cgaaactgca gccctgcgta ttttgtccct gaatccaaaa
     1981 tccctcaaat cgttgaactt cagataaatt tcataagcaa agtcctggcc tttctgccac
     2041 gcgattatgt cttcaaattt ttgaattgcc attttatttc tgttttcaat taacgatctc
     2101 ttatgccata aactttcgcc taaaacctta cgcctttctt atgacttacg cctatcgact
     2161 tacgcctgtt tatactgctc catcaggcgc tgcatgtatt ttccaagcac atcgaattcg
     2221 atgttcacca cggttccttt tttgaaattg tggaagttgg tattgtcgaa agtgtacgga
     2281 ataatagcaa cagaaaattt gccgggcttg ctgtcaacca ctgtcagact cacgccattc
     2341 acgctgattg aacctttggt cacggtgaaa ttattcagct tcgggtcgta ttcgaaatga
     2401 tatttccagc tgccgtcaaa ggtttcaacc ttgatgcagg tagctgtctg atcgacatgt
     2461 ccctgcacaa tatgtccgtc aaagcggcca tcggctttca tgctgcgttc gagattcacc
     2521 tgatagccct ttttccagtt gcgcaaacag gttttattga gtgtttcatc aatggcagtt
     2581 acaacatatt ctttgttttt cttgtcgact ttcaccaccg taagacaaac accgtcgtgg
     2641 ctcatgctct gatcaatctt caattctttg gtcagatccg attttacggt gaaatgtatg
     2701 ttacttcctt cgcgggttac ttttgtgatt tcgcccatgg attctacgat tccgctaaac
     2761 atagtgtgtc tgtttttttg tttgattatt ttttcattgc atcttcaact tccgcgatgg
     2821 tgatcgggag ccgttttccg ataaaacgag atccgccctc agtgaccaaa atatcgtctt
     2881 cgagccggat tccgccaaag gttttgaatt tctccacttc agcatagttg atgaattcct
     2941 caaatttctt ttcagctttc cactgatcga tgagttccgg aatgaaataa atcccaggtt
     3001 catcggtcat cacatgacct gccttgaggc ggcgtcccat gcggagatac gcggttccaa
     3061 actgatcgat gcggcttact tcgtcgtcgt aaccgacata attttcgccc agatcttcca
     3121 tatcgtgcac attcatgccc atcatatggc caagtccatg cgggaaaaac atggcatgag
     3181 cacccgcttt aacggcctca tccacattgc ctttcataag tccggcattt ttcagacctt
     3241 cggcaatcac acggctcacc gaaagatgca cactctgata agtcacttcg ggttttgtta
     3301 ccgagagagc atggttgttg gcatcgagca caatctgata aatcattttc tgctgctcgc
     3361 tgaattttcc gctcaccggc atagtgcggg tataatcaga tgtataatac ttattgcttt
     3421 gtgcaccggc atcgctcaaa agcagctgtc cggcctgcat cacatttgag tgattgtggt
     3481 tatgtaaagt ttcgccacga accgacaaaa taatcggaaa cgaaggacct ttgccgtatt
     3541 gataagcaac accttccatc agtccgcaga cttcctgttc aacaacgccg gggcgggcat
     3601 tgcaaaaagc aatcatatgc atgtcataac caatttttgc agccctttcc agttcttcaa
     3661 tttcgtactg ttctttaata gcgcgcaatg caacaactgc tttaatcaga tcgatacttg
     3721 ccttggtttt catttctgta aaaggaattc cgagcatggt attaagcagt atcttgcttt
     3781 cggcgcgata aggtggagta aaatgaatgg cacgacccgc attttttact ttaaccagat
     3841 aagattcgag ttcagcaaaa ggatatgttt ccacaatgcc aacctttgcg gcaagatctt
     3901 tcactgcggg ctgtggcccc atccagatga tgtcttcgat atcgacatcg ttggcaaaaa
     3961 ggatatgttt cccttcatca aaatcaatga taccaaccat atccggaata tcaaggccaa
     4021 agaaatagga aaaagtgctg tcctgacgaa aatgatatcc attggcagga taatccattg
     4081 gggattctgt atttccgata aacagtgcaa tgcctttaag cccgcttttc aagagacgtt
     4141 cacgatgtga aacataaacc tgtttttcaa agagttccat attgttgtgt tttttaatga
     4201 caataatttt ctcaaaagta atatttgttt gggaaatgag gaaggnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnncctgt aattatgaaa cagactttac tcaatcggtt
     4381 tttacgttat gttgcacttg acacgcaaag tgatccaaac agtgatacgg ttccaagcac
     4441 caacaaacaa cttacgcttg ccaatgcttt ggccaccgaa ctcaaaacct ttggagtgac
     4501 tgatgtatcg ctcgattcaa aagggtatgt tatggcttca ataccatcca atatcgaccg
     4561 agacattaca cccataggat ttatcgctca tatggacaca tcgcccgaca tgcccggcaa
     4621 cgatgttaag ccacagatta tagagagcta tgatggaggc gatattgtgc tgaatgcaga
     4681 aaaaaacatt catttgagtc ctgaggaatt tcccgatttg ctacgttata aaggacaaac
     4741 actcataaca accgatggaa caacgctcct tggcgccgat gacaaggccg gcattgccga
     4801 aatcatgacg gccatagaat atctgatgga acatccggtc atcaaacacg gaaaaatctg
     4861 tattgcattc acccctgacg aagaaatcgg tcgtggcgtt gatcattttg atgtaaaaaa
     4921 attcggagcc acctttgcct acactcttga tggaagcggt cccggtgaat tggaatacga
     4981 aaatttcaat gcagcttcag ccacaataaa aattaagggg aacaacattc atccgggcta
     5041 tgcaaaaaat aaaatgctca atgcatcatt gcttgccaac gaattcatct caatgcttcc
     5101 ggttaatgaa agacctgaat ttactgaaca ttacgatgga ttttttcatc tgaccggact
     5161 gacatcaacg gtagaagaag ctgaaatttc ctatatcata cgcgaccacg acaaggccat
     5221 ttttgagcgc cgcaaaaaac agatgcagga cattgttgac tacctgaacg gtaagtacaa
     5281 aaaagaaact tttctcctcg acattaaaga tcagtattac aacatgcgtg aaatgatcga
     5341 accggttttt catgttgttg aaattgcgat gcaggccatg cgaaatgtgg gtgtaaaacc
     5401 cgatgtaaaa cccatccgtg gcggtaccga cggaagccgg ctcagctaca tgggtttgcc
     5461 ttgtccgaac ctcttcgccg gcggccataa ttttcacgga aaacatgaat ttgttgctgt
     5521 agaatccatg gttaaagcag tagataccat tctcaatatt attgccattg tttctgcgaa
     5581 agaagcataa ttagcccgca gagaatggct cacagagaga gcaaaggcgc agagagattt
     5641 tacttaagca tcgcgcagag ggagtttagt ccgacgaagg cggaccagag gcgcagagga
     5701 atttgccacg aaggcacaac ttgtcgcgtt ttacgtgaag acacaaaaaa catacacctc
     5761 tgagttcccg gtcactaact gcgacttagc tttgctcact gagccgggat ctcctggaaa
     5821 accaacgcag ccattgctga atctacattt caggagaccc cctgacggta ccgtcagggg
     5881 gtcttgctgg tgtaggccgg gcggtcgcga tgaatactga ttcagtgggt cgcgcaaaga
     5941 atggctcgcg gagggagttt agtccgacga aggcggacca aagacgcaga gagattttac
     6001 ttaagcatcg cgcgaagagc gcagggacgc agagagattt gcatcaacgg ccgcgtttga
     6061 cattgggcgc gcgtctgcgc gtgcagcgtt tcacgttgac gcatgacctt gcatctcaaa
     6121 agagtttatg atcatcctta ataatgccag taatcgatta gggttgtcca acattccata
     6181 gttcgaagta ctgccctgca gtataaaatc cacgaagaac atggaaaagt tggatagaga
     6241 tctctgcaac tgactcttcc atgtcctcca ttgtatttcg gactattgaa ggtcgtgttg
     6301 ctttttactc agaaataaat ttcagattgt ctctgaagct ttatctggta tcattgcgga
     6361 tgtacaaaaa agagtttcac gtaaaatcac gtagaac
//
LOCUS       DAOPKE010000136         5975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_14278_length_5975_cov_10.329730, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000136 DAOPKE010000000
VERSION     DAOPKE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_5975_cov_10.329730"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1973
                     /locus_tag="PKY63_11975"
     CDS             <1..>1973
                     /locus_tag="PKY63_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF01377.1"
                     /translation="SMNIRGVFPENEKMNYMSGRALHPVSVHLAAKVQAEFDGMLDIS
                     FSAGADAFNVVDLLLCGLGPITMSSDLLKPGGYGRLQQYVKNIQSAVGHSNAGSLQSF
                     ICPPGKMRAVKLKDYTSETLENPAYRKAMREPSIKTARPLGLFDCIHAPCVNTCPDNQ
                     NIPQYLYHVSKGDFQKAFEVIMKTNPFPSVTGAVCDHACQTKCTRINYDENLAIREVK
                     RWVAEKQLDESFIQSRPKNGMKAAVIGAGPAGMSCAYFLLMAGFEVDVFEEKHFAGGM
                     AADVIPAFRLKNDAIEKDIKRIEKLGVKVHYGTRINKAIFDQFVNERRFVFIATGTPE
                     SKKLGVKGEDSMGVIDPLVFLSDLKKGNAVLPGRRVLVIGGGNTAMDVARAVKRMQGG
                     NGSVIIVYRRRMADMPAEAEEILAARAENIEFACLKAPVEIVVAGGKVGGLKVTTMRT
                     TGIGSDGREKVEAIPNSEEILIADAIFPAIGQNAALDFIDQSLLKKNSDNFSTLLDRV
                     FIGGDVRKGPASIIAAIADGRKAAEQMIAEAGIVDLANDLDSRGSNVNELMIRKAKRE
                     KSQLDEEVPGLPSKVIKNNQEAMIEAARCLLCDEVCNICVTVCPNLANQFYETKPFKF
                     TLGDYCFEIKQKIQTYNIGEFCNECGNCRTFCPT"
     assembly_gap    1974..2073
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2447..5368)
                     /locus_tag="PKY63_11980"
     CDS             complement(2447..5368)
                     /locus_tag="PKY63_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPF01378.1"
                     /translation="MKSILLFGAAVIFSASLNAQITQKAPVNPAYIQYIQSGSPASQY
                     GYLPPIIDLQAKQYTAPSKGPLVLPATFDLRTLGYVTSVKNQGGCGSCWTFATVASTE
                     SYWLRQGQGTFDLSEDNINNCHDFDWTPCNGGNAMISAAYVSRGGGMMTEASDPYNDV
                     AGTCPTTINPIKYVSDVRYVPDNTAIIKQILYDYGAVYTSYYSDDVYLNTSNDTYYYS
                     GTDYANHAVTLVGWDDTKVTDGGTGAWIVKNSWGTSWGENGFFYISYNDSRINSEAAY
                     FPGFRNFSNGDEVLTYAKYGEINDIGLGSTIGYGLINYSVSTNSIIKAVGTYIASGNS
                     TVSMEIYSDFDGTNLSNLIDTISSKSCSLPGYYTFDLPNPVVLMSGENIYIKVKYSTP
                     GYSYPIPVEEVYSGFASNVTLETGKCWVSSNGTTWNAAGSGTSREYDLCINLYTSAAG
                     IVTTAFSADVTSTCTGVVSFSDESINSPTNWLWTFGDGQTSTVQNPVHYYTSNGTFTV
                     KLKSWNIFGTDSLIRTSYVTVGLPAAPTATDASICSGQTATLTATGSGTLEWYDAQYG
                     GNLVFTGSSFTTPALSNSTDYFVQSAHIPGTSVYGGNQNSTSGGSNFTASSIHHLNFD
                     AAEDCRLISVEVNASTTGNRTITLKNSAGTVLQTATVNIATTGVSRITLNFEIPAGSG
                     YQLVGPAYPNLYRNNLNVSYPYEVSPISITGSSAGSEYYYFFYDWEVQPYCSSARTTA
                     SAVVSGPDNALNISGNYSICDGDSLTLTAASADAYYWYPNGETTQSITVNTAGNYYAL
                     ITGSSCDVYTDTVAVSLTSMPTVDFIWTINMNIAEFTSVSTHATNYYWEFGDGATSSQ
                     ANPAHYYGSGGIYTVNHYAWNACDTVMVSHQVSISTELEEHSSGFSMWPNPAGDLIQI
                     SVESEIPSYIRIINMQGQELKRIIPTEKETVIQLSGISSGVYILECAGSVVMRMRFVV
                     E"
BASE COUNT         1593 a         1235 c         1354 g         1693 t
ORIGIN      
        1 agcatgaata tcagaggtgt ttttccggaa aatgagaaaa tgaattacat gagtggtcgg
       61 gcattacatc ctgtatcggt tcatttagct gctaaagtac aggccgaatt tgatgggatg
      121 cttgatattt cattttcggc cggagccgat gcttttaatg tcgttgattt gctgctttgt
      181 ggtcttggcc ccataaccat gagctccgat ttattgaaac ccggcggcta tggccggctt
      241 cagcaatatg tgaaaaatat tcagtcagct gtgggtcatt ccaatgccgg gagtttgcag
      301 tcttttattt gtccgcctgg taaaatgcgg gcagttaaac ttaaagatta tacatccgaa
      361 acactcgaaa atccggcata tcgtaaagca atgcgcgaac cttcgattaa aacggccagg
      421 ccattgggtt tgtttgattg cattcatgct ccatgcgtga acacctgtcc cgataatcag
      481 aatattccac agtatttata tcatgtctcg aaaggtgatt ttcagaaagc gtttgaagtt
      541 ataatgaaaa ccaacccgtt tccttcggtg acgggagccg tgtgtgatca tgcctgtcag
      601 acaaaatgca cgcgtatcaa ctatgatgaa aatctggcta tacgcgaagt aaagcgttgg
      661 gttgccgaaa agcagctgga tgaaagcttt attcagtcgc gcccgaaaaa tggcatgaag
      721 gccgctgtta ttggtgccgg acctgctgga atgagttgcg cttatttttt attgatggct
      781 ggattcgaag ttgatgtatt cgaagaaaaa cattttgccg gtggtatggc ggctgatgtg
      841 attccggcat ttcgtctgaa gaatgatgcg attgaaaaag acattaagcg cattgaaaaa
      901 cttggggtaa aggtgcacta tggtaccaga ataaataaag cgattttcga tcagtttgtc
      961 aacgaacgtc gttttgtttt cattgctacc ggaacacctg agtctaagaa attgggagtc
     1021 aagggtgaag attcaatggg cgttatcgat ccgcttgtgt ttttatcaga tttgaaaaaa
     1081 ggtaatgctg ttttaccggg acgccgggtg cttgttattg gcggcggaaa tacagccatg
     1141 gatgtggccc gcgccgtgaa gcgtatgcaa ggcggaaacg gaagtgttat cattgtttat
     1201 cgccgccgaa tggccgatat gccagccgaa gcagaagaaa tactggctgc ccgtgccgaa
     1261 aacattgaat ttgcctgcct gaaagctccg gtggaaattg ttgtcgcagg tggaaaagtc
     1321 ggtggactta aagtgactac catgcgcaca accggcattg gatcggatgg acgcgaaaaa
     1381 gttgaagcta ttcccaatag cgaagaaatt ctgattgccg atgccatttt ccctgccatc
     1441 ggacaaaatg ctgctcttga ttttattgat cagtcattgt tgaaaaagaa cagcgacaat
     1501 ttctctacat tattggatcg ggtgtttatt ggcggcgatg ttcgtaaggg accagccagt
     1561 attattgccg ccattgccga tggacgtaaa gctgcagaac aaatgattgc cgaggccggt
     1621 attgttgacc ttgccaatga tcttgacagc cgtggttcca atgtcaatga gctgatgata
     1681 cgaaaggcaa aacgcgaaaa atcacagctt gatgaagaag tgcccgggtt gccttctaaa
     1741 gttattaaaa ataatcagga agccatgata gaggccgcgc gctgtctttt atgtgatgaa
     1801 gtttgtaata tctgtgtaac tgtttgtcca aatcttgcta atcagtttta tgagactaaa
     1861 ccattcaaat ttactttggg tgattattgt tttgagataa agcaaaaaat tcaaacttat
     1921 aatataggcg aattctgtaa cgaatgcggc aattgccgga ctttctgtcc gacnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattccat agttcggaga aaatcggaga
     2101 acatggaata gttggtctga gtgcggtaaa aacaatgaga aatatttggc gatgatgaga
     2161 attagaaatg tgaaaatgtg cggctcacgt gagacgtgac aggttcgccg atgtggaaat
     2221 gtgaaaacac gagatttttt cgcattattg cattatcgca ttaacgctat attagagcga
     2281 gagcgcaaaa aaatcatgct ccatgcacca tgctttgtgc ttttagcgta atgcattgtg
     2341 attgattgaa tccgactctt ccatgacctt catttcattt cggactctgg aaggtcggtt
     2401 cgtttgaaga agttattcta ttttgcaaac atacgagggt ttgtggctac tccaccacaa
     2461 accgcattct cataactacg cttccggcgc actccagaat atacactccg ctcgatattc
     2521 cgcttaattg tatgactgtt tccttttcag ttgggattat tcttttcagt tcttgtccct
     2581 gcatattgat gatgcggata tatgacggaa tttcgctttc aacgctgatc tgaattaaat
     2641 ctccggcagg attcggccac atactgaagc cggaagaatg ctcttccaac tcggtgctga
     2701 tactgacctg atgactcacc atcacagtat cacatgcatt ccatgcataa tgattaacgg
     2761 tgtaaatgcc acctgaaccg tagtagtggg cggggttggc ctgagatgag gttgctccat
     2821 cgccgaattc ccagtaataa tttgtagcat gggtacttac acttgtaaat tcagctatat
     2881 tcatatttat tgtccagatg aagtcaacgg taggcattga cgtaagcgaa actgcaacgg
     2941 tatcggtata tacatcgcag cttgagccgg taatcagcgc ataataattt cctgcagtat
     3001 tcactgtgat tgattgtgtg gtttcgccat ttggatacca gtaataagca tcggcactgg
     3061 ctgcagtcaa tgtaagtgaa tcaccgtcac agattgaata attgccactg atgttcaatg
     3121 cattgtcagg gccactgact actgcactgg cggtagtgcg ggcacttgag caatagggct
     3181 gtacttccca gtcgtagaag aaataataat attccgaacc ggcactgcta cctgtgattg
     3241 aaatcggaga tacttcgtat gggtatgaaa catttaaatt gtttctgtac agatttgggt
     3301 aagctggtcc aaccaactga tatccgcttc cggctggtat ttcaaaattc agtgtaatgc
     3361 ggcttacgcc tgttgttgct atatttactg ttgcagtttg aaggactgtt cctgcgctgt
     3421 tttttaatgt tattgttctg tttcctgttg tgctggcatt cacttcaacc gaaatcaacc
     3481 ggcaatcttc agctgcgtca aaattcaaat gatgaatgga tgatgccgtg aaattgctgc
     3541 caccggaggt ggaattctga ttgcctccat aaaccgatgt tcctggtata tgggctgact
     3601 gaacaaaata gtcggtgcta ttgctgagtg ctggtgttgt aaaactgctt ccggtaaata
     3661 ccagattgcc cccgtattgg gcatcgtacc attccagtgt tccgcttcca gtggcagtca
     3721 atgttgcagt ttgtccgctg cagatagatg cgtcggtggc agtaggtgct gccggaagtc
     3781 ccactgttac atacgaagtc cggattaaac tatcagttcc gaaaatattc cacgatttca
     3841 attttacggt gaatgttccg ttgcttgtat aataatgcac cggattctgc actgtcgatg
     3901 tctgtccgtc gccgaaggtc cagagccagt ttgtgggcga attgatgctt tcatcgctaa
     3961 acgaaacaac acctgtgcat gtagaagtta catcggcaga aaaagccgtg gttacaatgc
     4021 ccgcagccga agtataaaga ttaatgcaaa gatcgtattc gcgcgatgtg ccgctgccgg
     4081 ctgcattcca ggttgttccg tttgaactta cccagcattt tcctgtttca agagttacat
     4141 ttgatgcaaa accactgtaa acctcctcaa ccggaatcgg ataactatag ccgggtgtcg
     4201 agtattttac tttgatgtag atattttctc ctgacataag aacaaccggg tttggcaggt
     4261 cgaatgtgta gtatccgggc agactgcacg atttgctgct gatcgtatca ataagattac
     4321 tcaggttggt tccatcaaaa tcggaataaa tttccatact gaccgttgaa tttcccgatg
     4381 caatgtaggt ccctacggct ttaatgatcg agtttgttga tacagaatag tttatcaaac
     4441 catatccgat agtgctgcca aggccaatgt catttatttc gccgtattta gcgtaagtga
     4501 gtacttcatc accattggaa aaattacgaa atccggggaa ataggcggcc tcggaattta
     4561 ttctcgagtc gttgtaactg atatagaaaa aaccgttttc gccccaagaa gtgccccagc
     4621 tgttttttac aatccatgcg ccggtacctc catcggtaac ttttgtatca tcccagccca
     4681 ctagggttac tgcatgattg gcatagtcgg ttcctgaata ataataggtg tcgttcgaag
     4741 tattcagata gacgtcgtcg ctgtaatatg aggtataaac tgcaccgtag tcatataaaa
     4801 tttgtttgat gatggctgta ttgtctggca catagcgcac atcgctgaca tatttaatgg
     4861 gattgatggt agtcggacat gttccggcaa catcgttata cgggtctgac gcttcggtca
     4921 tcatgccgcc gccgcgcgaa acataggcgg ccgaaatcat ggcgttacct ccgttgcatg
     4981 gtgtccagtc gaaatcgtga caattattga tgttgtcttc tgataaatca aatgtgcctt
     5041 gtccctggcg cagccagtac gattcggtcg atgctactgt ggcaaaggtc cagcaggaac
     5101 cgcatccacc ctgatttttc accgatgtca cataaccaag tgtacgcaaa tcaaaagtag
     5161 ccggtaagac caacggtcct ttggatggag ccgtgtattg ttttgcctga agatcaatga
     5221 tcggcggcag atatccgtat tgcgatgccg gactgccgga ctgaatgtat tggatataag
     5281 ccggattcac cggagctttc tgtgttatct gagcgttgag cgaagctgaa aaaatcacag
     5341 cagctccaaa aagtaatatt gatttcataa atcggtgctt ggttatttaa agacagcgaa
     5401 agcaaatata cgttgctttg aaatataaaa aggatgaggt caaggacgaa aagttgtcag
     5461 cattcagtta tcagccatca gctctgagac tgggccgaga agattgagga caaaggcaag
     5521 aactctgaac aaggaacaag gaagtgagaa tgccgaacgg ttcttcatta tattttcatt
     5581 caaactacac ttcaaacttc taacatcctt gttccttgtt cattattcaa tgaggtaagg
     5641 aactctgatc attccgacgc tttcggtcgg aactgtcagc tgaacaatac gtgcatcctt
     5701 acggatcaag aggaagtgag aatatcgaac ggaactacag tatattctta ttcaaacctc
     5761 atttcttaat tcacttcaaa cttcgtacat ccttgttcct tgttcatgat ttaacgtttt
     5821 cgtgaaatag ccgcttatcc tcaaccttat ccttatcctc aacctgaacc tcaaccttaa
     5881 cctcaacctt aacctcaacc ttaacctcaa ccttaacctc aacctcaacc ttaacctcaa
     5941 ccttaacctc aaccttaacc tcaaccttaa cctca
//
LOCUS       DAOPKE010000137         5972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_14286_length_5972_cov_13.178807, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000137 DAOPKE010000000
VERSION     DAOPKE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14286_length_5972_cov_13.178807"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKY63_11985"
     CDS             <1..567
                     /locus_tag="PKY63_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF01379.1"
                     /translation="INVLINNAGMSMRAIFEDLDLKVIEQLMNVNFWGTVFCTREAIP
                     FLLKSKGSVVGVSSIAGFQGLPARTGYSSSKFAMHGFLNTLRVETLNQGLHVMIACPG
                     FTESNIRNTALTADGSPQGSTPLDEKHLMSADDVAGRIIRGIEKRKRTLVMTTQGKLV
                     VLLGKFFPKWVDKMTYKTMKKEKNSPLK"
     gene            586..1017
                     /locus_tag="PKY63_11990"
     CDS             586..1017
                     /locus_tag="PKY63_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01380.1"
                     /translation="MKTEKIKILPMEGCSLIRFGASENDVINAIGEPDERENIDDEDY
                     NSEIWYYDDKGVTLFLDEMEDEGLLVSGIELEDECELEGVSIMGKSIDEIKAIADKHK
                     FGEADVEKEEWGEFRLSYEDKLLDFYFDENKKLISVSLGME"
     gene            complement(1068..1982)
                     /gene="xerD"
                     /locus_tag="PKY63_11995"
     CDS             complement(1068..1982)
                     /gene="xerD"
                     /locus_tag="PKY63_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPF01381.1"
                     /translation="MKTRNIQDPVIKSFSTFLMLEKALSEHSIEAYIHDVGLFMRWNE
                     QIATNKPLRDIHKNDIHSFVQWLSDISLTPRSQARIISGIKSFFAFCLMDGLTDNNPT
                     ELIELPRLGKHLPTVLSLDEIEKMIKCIDLSKADGHRNKAIIETLYACGLRVSELVNL
                     SFTDLFFDDEFIRVIGKGNKERLVPIGHSAMNALKLYIEKSRVFFPVVRNHEHFIFLN
                     QRGKQLTRASVFNLVKVLAIATGIKKNISPHTFRHSFATHLIENGADLRAVQELLGHS
                     SITTTEIYTHISREFLRDVVLKHHPMYR"
     gene            complement(1969..3981)
                     /gene="uvrB"
                     /locus_tag="PKY63_12000"
     CDS             complement(1969..3981)
                     /gene="uvrB"
                     /locus_tag="PKY63_12000"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPF01382.1"
                     /translation="MKKFELISDFVPTGDQPEAIRQLTNGIKDGELFQTLLGVTGSGK
                     TFTVANVIANTNRPTLVLSHNKTLAAQLYSEFRQFFPDNAVEYFVSYYDYYQPEAYLP
                     ISDTYIEKDLSINEEIEKFRLSATSSLLSGRRDVIVVSSVSCIYGIGNPQDFTTHVIE
                     LEVNEKKTAKFVVNALVDALYSRNDIELKRGTFRLKGDTLDVYPAYADTAYRIIFFDK
                     EIESIASFDPVTGRSIEKMEMLRIYPASIFMTTKDKLSSALNEIYVDLGAQCDFLRQI
                     GKDIEAKRLEDRVNYDLEMMKELGYCSGIENYSRYFDSRKPGSRPFCLIDYFPDDFLL
                     VVDESHATIPQVRGMYGGDRSRKQVLVDFGFRLPSAMDNRPLKFDEFEALLNQTIFVS
                     ATPADYEFMKCEGAYVEQVIRPTGIPDPEVLVRPSNNQVDDLLNEIKLQIEKDERTLV
                     TTLTKRMAEELTKYLFELNIPCKYIHSDVETFERVEILQELRRGDIKVVVGVNLLREG
                     LDLPEVTLVAILDADKEGFLRNERSLIQTAGRAARNIKSRVILYAMKMTTSMQKMIEE
                     TERRRNKQLEYNKANNITPVSVHKAIGDSFEGARKIEMKYYVEPEENRLAADPLVPYM
                     SKDALKKRIDAVKKEMEKAAKELDFITAAHHRDEMLALQKMLHENA"
     gene            complement(3978..5102)
                     /locus_tag="PKY63_12005"
     CDS             complement(3978..5102)
                     /locus_tag="PKY63_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPF01383.1"
                     /translation="MAKTESKRGGFMERILWIAVVSFSVLLLISIISPLCNPDRFWVP
                     AFFGLAYPYIALLTVISAIVMALFNFKKAIFPLLIFLIGLGPCKHTMNVSGQKNNFSE
                     EETDIEILSQNVHLMGIYDTSGIITIDSLLNVITNESPDIACFQEFYLREKYTGKNCE
                     PFMQAGQFSDYTTSLYADSTSDTYLSLITFTRFPVINQGVVRTPDHSERNIFSLWTDL
                     KINEDTIRIYNIHLQSIGFTENEESLFDEKEEENGELERKSKSTARKLKRAFVARSQQ
                     VRILATHIDSCRHPVFLAGDFNDTPVSYAYRKLRGELNDAFTDSGTRGMGKTYNGRFP
                     SFRIDYIFYPKEFEAAEFKVIGRRYSDHFPISCRFKKKAE"
     gene            complement(5175..5849)
                     /gene="trmD"
                     /locus_tag="PKY63_12010"
     CDS             complement(5175..5849)
                     /gene="trmD"
                     /locus_tag="PKY63_12010"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPF01384.1"
                     /translation="MRIDILTLFPEMFTGPFTESIVKRAVVKGLIEIHIHNIRDFSKD
                     KHQRVDDYQFGGGAGMVMMAEPVIDAIESLTSVRQYDDIIFMTPDGEKFSQPLANQLS
                     MCENIILLCGHYKGLDERVREHFITREISIGDYVLSGGELAACVVADAIIRLIPGALN
                     DETSALSDSFQDGLLSAPVYTRPAEFRGWKVPDILLSGHEKNIHEWRQQQSLERTKKR
                     RPDLMD"
BASE COUNT         1695 a         1264 c         1253 g         1760 t
ORIGIN      
        1 atcaatgtat taatcaacaa tgccggtatg agtatgcgcg ccattttcga agatcttgat
       61 ttgaaagtga tagagcagct tatgaatgtc aatttctggg gaactgtttt ttgtacacgc
      121 gaagccattc cttttttatt gaaatcgaaa ggcagcgtgg tgggagtctc atccattgca
      181 ggttttcagg ggttgcccgc acgtaccgga tattcatcat cgaaatttgc catgcatgga
      241 tttctgaaca cattgcgtgt tgaaacactg aatcagggtt tacatgtgat gattgcctgc
      301 cccggattta cggagtcaaa tatccgaaac accgctctca cagccgatgg cagcccgcag
      361 ggctcgactc cgcttgacga aaaacatctg atgagtgccg atgatgttgc aggcagaatt
      421 attcgcggta ttgaaaaaag aaaacgtact cttgtgatga caacacaagg aaaactcgtg
      481 gtattgcttg gaaaattttt cccgaagtgg gttgacaaaa tgacctacaa aacaatgaag
      541 aaagaaaaga attcacctct gaaataaacc aaaaaaaata caaccatgaa aactgaaaaa
      601 atcaagattc ttccgatgga aggatgcagc ctgataaggt ttggcgcttc tgaaaacgat
      661 gtaatcaatg ccattggcga acccgatgag cgcgaaaaca tcgatgacga agattacaac
      721 agcgaaatct ggtattacga cgacaaaggt gtgacgctgt ttctcgatga aatggaagac
      781 gaagggctgc tcgtttcagg tatcgaactt gaagatgagt gtgaacttga aggcgtttcc
      841 atcatgggaa aatccattga tgaaataaag gcaattgccg ataaacacaa attcggtgaa
      901 gctgatgttg aaaaagagga gtggggcgaa ttccgccttt catacgaaga caaactgctt
      961 gatttctact tcgatgagaa caaaaagctc atcagtgtga gtctgggtat ggaataaaaa
     1021 gtagtgcaaa gtttactgcg ctgtgcgaag ttatccaact tcgcacacta cctatacatc
     1081 ggatgatgtt tcaataccac gtcgcgcaga aattcgcggc tgatgtgggt gtaaatttca
     1141 gtggttgtaa tgctgctgtg accgagcaat tcctgtacgg cgcgcagatc ggcgccgttt
     1201 tcaattagat gtgtcgcaaa agaatgccgg aaagtgtgcg ggctgatatt cttttttatt
     1261 ccggtagcga tggccaatac tttcaccaga ttgaaaactg atgcgcgtgt aagctgcttc
     1321 ccgcgctgat tcagaaaaat gaaatgttcg tgattccgta caaccggaaa aaatacgcga
     1381 cttttttcta tataaagctt taatgcattc attgctgaat gtccgatggg aacaagtctt
     1441 tccttatttc cttttccgat tacacgaatg aattcatcat cgaaaaataa atcagtaaaa
     1501 gataaattta caagctcaga tactcgtaaa ccgcatgcat acaatgtttc aataatggct
     1561 ttgtttctgt gtccgtctgc ttttgataaa tcgatgcact ttatcatttt ctcaatttcg
     1621 tcgagtgaaa gtactgttgg caagtgtttt cccaaacgtg gaagttcaat cagctccgtt
     1681 ggattattat ctgtaaggcc gtccataagg cagaatgcaa aaaatgattt aatgccggat
     1741 atgatacggg cctgcgatcg tggtgtgagc gaaatatcgc tcagccattg aacaaaagaa
     1801 tgaatatcgt ttttatggat gtcgcgcaat ggcttatttg ttgctatttg ctcattccag
     1861 cgcataaaca atccaacgtc atgaatgtaa gcttcaatcg aatgttccga caatgctttt
     1921 tcaagcatca gaaaagttga aaaacttttg ataacaggat cctgaatatt acgcgttttc
     1981 atgcagcatt ttttgtaaag ccagcatttc gtcgcggtga tgtgcggcag taataaaatc
     2041 cagttctttc gcagcttttt ccatttcttt ttttactgca tctattcttt ttttcaacgc
     2101 atctttgctc atatatggca ccagcggatc tgcagccagt cggttttctt cgggctctac
     2161 atagtatttc atttcaattt tgcgggcacc ttcaaaacta tcaccaatgg ctttatgtac
     2221 cgaaaccggc gttatattgt tggctttatt gtattcaagc tgtttgttcc tgcgtcgctc
     2281 agtttcttca atcattttct gcatcgaagt agtcattttc atggcatata aaatcacccg
     2341 gcttttgata ttgcgcgctg cacggcctgc tgtttgaatc agactccttt catttctcag
     2401 aaatccttct ttgtctgcat caagaatagc taccagagtg acttccggca aatcaagtcc
     2461 ttcccgaagt agattgacac caacgaccac ttttatgtct ccacgtcgca attcctgaag
     2521 aatttccacc ctttcaaatg tttccacatc actgtgaatg tatttgcatg ggatgttcaa
     2581 ttcaaacaga tattttgtca actcttctgc catgcgcttg gtgagcgttg taaccagagt
     2641 gcgctcatct ttttcaatct gaagttttat ttcattcagt agatcatcta cctgattgtt
     2701 cgatggacgt accaacactt caggatctgg aataccagtc gggcggatta cctgttccac
     2761 ataagcgcct tcgcatttca taaattcata atcagccggt gtggccgaca caaatatcgt
     2821 ttgattgagc aaagcctcaa attcatcaaa ctttagcgga cggttgtcca tcgccgatgg
     2881 caggcgaaat ccgaaatcaa caagtacctg cttgcgtgag cggtcaccgc catacatgcc
     2941 gcgcacctgc ggaatagttg catgactttc atcgacgacc agcagaaaat catctggaaa
     3001 ataatcaatc agacaaaaag gtctggatcc gggtttgcgg ctatcaaaat atcttgaata
     3061 gttttcaatg cctgaacaat agcccaattc cttcatcatt tccaggtcat aattcacgcg
     3121 gtcttccagt ctttttgctt caatgtcttt tcctatttgt ctcagaaaat cacactgagc
     3181 gccaagatca acatatattt cattcagagc cgaggatagt ttgtctttgg ttgtcataaa
     3241 tatgctggcc ggataaatcc gcagcatctc cattttttca atactgcgcc cagtaaccgg
     3301 atcaaacgat gcgatgcttt caatttcctt gtcaaaaaaa atgatgcggt atgccgtgtc
     3361 cgcatacgca ggataaacat ccagggtgtc cccttttaat cggaatgtac cgcgtttcaa
     3421 ctcaatgtcg ttgcgcgagt acaatgcatc tacaagcgca tttacaacaa attttgccgt
     3481 tttcttttca ttgacttcaa gctcaataac atgtgttgta aaatcctgtg ggttgccaat
     3541 accataaatg cacgataccg atgaaacaac gattacatca cgtcgtccgc tcagcaacga
     3601 tgaagttgca ctgagtctga atttttcaat ttcttcgttg atggataaat ccttttcaat
     3661 atacgtatcg ctgatgggaa gatatgcttc gggctgatag taatcgtagt acgatacaaa
     3721 atattcaaca gcgttatccg gaaaaaactg tctgaactct gaataaagtt gtgcagccag
     3781 tgttttattg tggctgagca ccaatgttgg tcggtttgta ttggcaatca cattggcaac
     3841 ggtaaaagtc ttaccagagc cggtaacgcc caacaatgtc tgaaacagct ccccgtcttt
     3901 gattccattg gtcagctgac ggatggcttc aggttggtca cctgttggaa caaaatcact
     3961 tatgagttca aattttttca ttccgctttt tttttgaaac gacaactgat tggaaaatga
     4021 tcactgtagc gtcgtccgat caccttaaat tcagctgctt caaattcttt cggataaaaa
     4081 atataatcaa tccgaaacga tggaaaacga ccgttgtatg ttttccccat tcctctggtg
     4141 ccgctgtcag taaaggcgtc gttcagctct ccgcgcagtt tccggtatgc ataggaaaca
     4201 ggtgtatcat tgaaatcacc agcaagaaat accggatgcc gacatgaatc tatatgggtc
     4261 gcaagaattc tgacttgctg tgaacgggct acaaatgccc gtttcaattt gcgtgcagtc
     4321 gatttcgatt ttcgctcaag ctctccgttt tcttcttctt tttcatcaaa cagactttct
     4381 tcgttttctg taaatccgat cgattgaagg tgaatattat atattctgat tgtatcttca
     4441 tttattttta aatcagtcca cagagaaaag atatttcttt cggagtgatc tggagttctt
     4501 actacacctt gattaatgac aggaaatcgg gtgaaagtaa ttaaactcag atatgtatcg
     4561 ctggttgaat cagcatacaa cgaagtggtg taatcactga actggcctgc ctgcataaat
     4621 ggttcacagt tttttccagt gtatttttca cgaagataaa actcctgaaa acatgctata
     4681 tctggacttt catttgtaat tacattcagc agcgagtcaa tggtaataat cccgcttgtg
     4741 tcgtaaatgc ccatcagatg cacattttga gataaaattt caatatctgt ttcttcttct
     4801 gaaaagttgt ttttctgacc actgacattc attgtgtgtt tgcagggacc caatccaatt
     4861 aaaaaaatca ataagggaaa aatagctttt ttgaaattga ataatgccat aacaatcgct
     4921 gaaattactg ttagcagtgc gatgtaaggg taggcgagtc caaaaaaagc agggacccag
     4981 aaccggtcag gattgcaaag tggcgatatt attgaaatca ataataaaac agaaaaagaa
     5041 acaacagcaa tccagagaat gcgttccata aatccacctc gtttgctttc tgtctttgcc
     5101 ataataagtt caatacaaaa ttagtattat ttggtaaccc cttgaagggt ttcaaacctt
     5161 tcgaggggtt tatatcaatc catcagatcc ggccgccgct tttttgttcg ttcgagtgat
     5221 tgctgctgac gccattcatg gatgtttttt tcgtgtcccg ataaaagaat gtcgggtact
     5281 ttccatccgc ggaattcggc cgggcgcgta taaacgggtg ctgagagcag tccatcctgg
     5341 aatgaatcac tgagggctga ggtctcatcg ttgagtgcgc caggaatcag gcggatgatg
     5401 gcatcggcca ccacacatgc agccaattcg ccgccgctga gtacatagtc gccaattgaa
     5461 atttcgcgcg tgatgaaatg ttcgcgaacc cgctcgtcga gccctttgta gtgaccacaa
     5521 agcagaataa tgttttcgca catactcagc tggttggcca atggctgtga aaatttttca
     5581 ccgtccggcg tcataaaaat gatgtcatcg tattgtctga ccgaagtaag tgattcaata
     5641 gcatcaatga cgggttcggc catcatgacc attccggcac cgccaccaaa ctgataatca
     5701 tcgacccgtt gatgtttgtc tttcgaaaaa tcgcggatat tatgaatgtg aatttctata
     5761 agacctttga caacagctcg ctttacaatc gactcggtaa atgggccggt gaacatttcg
     5821 ggaaacaatg tgagaatatc aatgcgcata taggtttaaa agacaaaggt aggaatattt
     5881 gcacaacgtc gcgccttcgt tggaataaac ttcaggtata acgcatgcgg cgagctgctt
     5941 gcggaacaac gtcttcagat gaatgcttaa ta
//
LOCUS       DAOPKE010000138         5816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_14821_length_5816_cov_11.384482, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000138 DAOPKE010000000
VERSION     DAOPKE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_5816_cov_11.384482"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..517
                     /locus_tag="PKY63_12015"
     CDS             104..517
                     /locus_tag="PKY63_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01385.1"
                     /translation="MKKVNFVFYFLAIIAMFFSSCSKDASIREVKLCNNPSGDQCVED
                     MTDFFGVETDTIHLFASLTDAPENVKVEVTWFYQAVDKDKEEIMRGSFDGNESSTIHA
                     MLYLNTPGWPSGEYSVNIKIADSESEPVEKKFKIE"
     gene            520..930
                     /locus_tag="PKY63_12020"
     CDS             520..930
                     /locus_tag="PKY63_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01386.1"
                     /translation="MKRQILLIAVLALFATSCNMEPSIDHLRVCNNPDYDITCPSDHS
                     VLDAEETDKIYVTATMSHVPQNTAMTITWFYLEGGETEIDDVTLKTDEDMLDTPVYSY
                     LSAPYDGWPLGKYKVTIDLDMDNFTAVEKEFSIE"
     gene            1037..2128
                     /gene="aroC"
                     /locus_tag="PKY63_12025"
     CDS             1037..2128
                     /gene="aroC"
                     /locus_tag="PKY63_12025"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPF01387.1"
                     /translation="MPSGNTFGKLLSITLFGESHGPATGAVIDGFPAGIKINHDLIDM
                     MLKQRAPSAHFETKRREDDSVEFLSGVQKGVTTGAPVAFFIRNSDARSADYDYLKDVF
                     RPGHADYVGFKKYGNLPQPGGGRASGRETVARVVAGAFALDFLRKQKINIISYVSAIG
                     AARMKNSGQWFNEKAVFDAALHCPDKSAEAAMQKVIDDVAAARDSIGGLITTVVKGLP
                     AGVGEPVFGKLNAVLSQAVMSIPGAKGVDFGEGFSGIHLRGSQYNDAMQAEKGVTRFE
                     SNHSGGIQAGISNGNLMVMNTVFRPASSIGIHQNTVDIEGNEKTIIVGGRHDACFVPR
                     AAIVVTSMTALAVMDLWLQFKSFERKFGI"
     gene            2125..4158
                     /locus_tag="PKY63_12030"
     CDS             2125..4158
                     /locus_tag="PKY63_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPF01388.1"
                     /translation="MKYLKFTFLFFLFFSVSVIHAQDEEPCGKVSKKVAKQFDEAMSN
                     YAQARKMAAKLPASADRYYLEANRLLKEVIEVDAEFAPAYYYLGCINVFKKENNLTAA
                     EKYFKKAIEFCPDGMHDAYFQLGKIYFGLDKYDQAELNLKKYLDKPEEITDDSIQIEA
                     AEMYDWAKTAMELLSKPVSFDPEIVPGISSKFDEYLVIISPDNEKAFYTRRIEVKDMS
                     SWASEMELKEKFFVSNRKPQLGKPLSEIPFDSGQPMPDPFNMKLNEGGATVTIDNKEL
                     IYTICHMPERTDPNQYINCDLYYTRFEFGGWSDIIPIDAVNTSDHWESQPSISSDGKT
                     LYFISDRPGGIGGYDIYTSTRDESGSWSAPQNMGKIVNSEGNEKSPFIHTDSQTLYFS
                     SEGRPGMGGYDIYYARMDDNGTWQKPVNIGYPINSKYDDVGFFVSTDGKYGYFGSNNM
                     SEGLGGWDFYSFELYEEARPEKVLFVKGTVKNEQDDKPVEAKIEIKNLTTKEIREIPV
                     DHETGEYVAAIKFESDFMLTVKKPDFVYESTYISKEDSSFAEPTSVDVDLEPVQLGKA
                     YRMKDIYYESNSADLTAESKKVLDEFIEFLNENPNIKVKIQGHTDNIGGDAFNLTLSE
                     NRAHSVYEYLVSRGVSSSRLSYKGFGETQPVDTNDTEAGRAMNRRTEFLVTGM"
     gene            4400..4786
                     /locus_tag="PKY63_12035"
     CDS             4400..4786
                     /locus_tag="PKY63_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01389.1"
                     /translation="MEKDLLRLVLPEKLLDHFTITEVKEVFDNATKKMCYHILLEEHN
                     KIPEGYNEKEYESKGFYPAKTVQDFPIRGKAVFLIITRRRWRHKERKEEVIHSDYTFI
                     AEGSRITKELSDFLKYTGRYARGHDK"
     gene            4821..5738
                     /locus_tag="PKY63_12040"
     CDS             4821..5738
                     /locus_tag="PKY63_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPF01390.1"
                     /translation="MRRQYKKHGSGFQEWDQKTHATQYMIFPENIGEYISIDEVSLSQ
                     GELYTFVTNKNGHGRNGSLIASIKGTLSKDIISVLEKLPLEKREMVKEVTLDMAKNME
                     SSARQMFPKSKLVTDRFHVVKLVIDALQHVRINLRWTELDKENNAIAEAKKLNQKYEP
                     VVFENEDTPKQILARSRYILTRKPTDWTDSQKQRAAILFREYPLLKTAYEHTMEFRSI
                     YECKIEKYAKKRFLDWIEKTHSLLIKEFYTVANTVKINFDNILNFFINRSTNAFAESF
                     NSKIKLFRANQRGVVDTSFFLFRLHKLFA"
BASE COUNT         1789 a         1020 c         1382 g         1625 t
ORIGIN      
        1 ccttcgaggg ctgataactt ccttcgaggg ctgtttaaac aatatttttg tccggttttg
       61 tgtttataat aaatatttaa ttaggtttgt aaaaaatcaa aatatgaaaa aagtcaactt
      121 tgttttttac tttttggcaa ttattgcaat gtttttttct tcatgcagca aggacgcttc
      181 catcagggag gtgaagctgt gcaacaatcc ttcgggagat cagtgtgttg aagacatgac
      241 agattttttt ggtgttgaaa ccgacaccat tcacttgttt gcttcattga ccgatgcgcc
      301 ggaaaatgtg aaagtcgaag tcacctggtt ttatcaggcc gttgataagg acaaagaaga
      361 aatcatgcga ggcagttttg atggcaacga aagttcgacc atacatgcca tgctttatct
      421 gaatactccg ggatggcctt cgggagagta ttcggtgaat attaaaattg cagacagtga
      481 atcggaacct gttgaaaaga aattcaaaat cgaataaata tgaaaagaca aattttactg
      541 attgccgtct tggcattatt tgccacatca tgtaacatgg agcccagcat tgatcatttg
      601 cgtgtttgca acaatccgga ttatgatatc acatgtcctt cggaccacag tgtgctcgat
      661 gctgaagaaa cggacaagat ctatgttaca gccactatga gtcatgttcc tcaaaatact
      721 gcaatgacca ttacatggtt ttatcttgaa ggtggcgaaa ccgagattga tgatgttact
      781 ttgaaaacgg acgaagacat gctcgacacg ccggtgtatt cttacctgtc agccccgtat
      841 gatggatggc cacttggtaa atacaaagtg acaatcgatc tggatatgga taatttcact
      901 gctgttgaaa aggaattttc cattgaataa tctgtattct gttttacgag aattattcat
      961 atttagtaac aatcggctgt tgatactcgt aaaccggctg ttggatttac aacgacatat
     1021 ttagtagttt tgtatcatgc cgtcaggaaa tacatttggg aaattgctca gcattacact
     1081 ctttggtgag tcgcacggac ccgcaacggg agcggttatc gatggttttc ctgccgggat
     1141 aaaaatcaat catgatttga ttgacatgat gcttaaacaa cgcgctccct cagctcactt
     1201 cgaaacaaaa cgcagggaag atgattctgt tgagtttctg agtggagtac agaaaggtgt
     1261 aacaacagga gcgcctgtgg cttttttcat ccggaattca gatgcccgtt cagcagatta
     1321 tgattatctg aaagatgttt ttcggccggg tcatgctgat tatgtaggtt ttaaaaagta
     1381 tggaaatctt ccgcagccag gtggcgggcg tgcttcgggc cgtgaaacag tagcccgtgt
     1441 tgttgcaggc gcttttgctc ttgacttttt gcgaaagcag aaaataaata tcattagtta
     1501 tgtttcggct ataggagcgg cccgcatgaa aaacagcggt cagtggttta acgagaaagc
     1561 tgtgtttgat gctgcattgc attgtcctga caaaagtgct gaagcagcca tgcagaaagt
     1621 aattgatgat gtagcagctg caagggattc aattggaggt ctgattacaa ctgtggtaaa
     1681 agggcttccg gcaggagtgg gtgaaccggt tttcggtaag ctgaacgcag tgttgagtca
     1741 ggcggttatg agcattcccg gggcaaaggg tgttgatttt ggtgaggggt tttccggcat
     1801 tcatttgcgc ggaagtcaat acaatgatgc gatgcaagcg gaaaaagggg taacacgttt
     1861 tgaatcgaat cacagcggag gtattcaggc cgggatttcc aacggcaatc tgatggtgat
     1921 gaatacggtg ttcagaccgg cttcttcgat aggtattcat cagaatacag tggacattga
     1981 aggcaatgaa aaaacaatta ttgttggtgg acggcatgat gcttgctttg tgccgcgcgc
     2041 cgcaattgtg gttacatcga tgacggcgct tgctgtcatg gatttatggc tgcagtttaa
     2101 atcgtttgaa cggaagtttg gaatatgaaa tatctgaaat ttactttttt gttttttctg
     2161 tttttttctg tttctgtgat tcatgcacag gatgaagaac cttgtgggaa ggtgagtaaa
     2221 aaggttgcaa agcaattcga tgaagccatg tcgaattatg cgcaggcccg gaaaatggcg
     2281 gctaaactgc cggccagtgc cgatcgctac tatcttgaag ccaatcgttt attgaaagaa
     2341 gtaattgaag tggatgctga atttgcccct gcatattatt atctgggttg catcaatgtt
     2401 tttaaaaaag aaaataatct gactgcggct gaaaaatatt tcaaaaaagc gatagagttt
     2461 tgtcccgatg gtatgcacga cgcttatttc cagcttggaa aaatttattt tggactcgat
     2521 aaatacgatc aggccgaact taatctgaaa aaataccttg ataagccgga agaaataacg
     2581 gacgattcaa tacagattga agctgcagaa atgtatgatt gggctaaaac ggccatggaa
     2641 ctgctttcta aaccagtgtc attcgacccg gaaattgttc ctggtatcag cagtaagttt
     2701 gatgagtatc ttgtcattat cagtcccgat aatgaaaagg cattttatac gcgccggatt
     2761 gaagtaaaag atatgagttc ctgggcaagt gaaatggaac ttaaagagaa attttttgtc
     2821 agtaaccgga aaccacaatt ggggaagccc ttatcggaaa ttccattcga ttccgggcag
     2881 ccaatgccgg atccgtttaa tatgaaactg aatgaaggcg gtgctaccgt gaccattgat
     2941 aacaaggaac tgatatatac aatttgccat atgcctgagc ggacagatcc gaaccaatat
     3001 atcaattgtg acctttatta tacccgtttt gagtttggtg gttggagcga tattattcct
     3061 atcgatgcgg taaatacgtc tgatcattgg gaaagtcaac cttcgatttc aagcgatgga
     3121 aagactttgt actttatcag cgaccgtcct ggtggtattg gcgggtatga tatttatacc
     3181 agcactcgcg atgaaagtgg ctcgtggagc gctccgcaga atatgggtaa gattgtgaat
     3241 tccgaaggaa atgaaaaaag tccttttatt cacaccgaca gccaaacttt gtatttcagc
     3301 tctgaaggcc ggccaggtat gggtggctac gacatttatt atgcacgcat ggatgataat
     3361 ggaacctggc aaaaaccagt aaacatagga tatcccatca attcaaaata cgatgatgtc
     3421 ggattttttg tcagcaccga tgggaaatat ggatacttcg gttcaaacaa tatgagtgaa
     3481 ggacttggcg gttgggattt ttattcgttt gagttatacg aagaggcgcg cccggagaag
     3541 gttttgtttg ttaaaggaac cgtgaaaaat gaacaggatg ataagccggt ggaagcgaaa
     3601 attgaaatca agaatcttac cactaaagaa atcagggaaa ttcctgttga tcatgaaaca
     3661 ggcgaatatg ttgctgccat taagtttgaa tcggatttta tgctcacagt gaaaaagccg
     3721 gattttgttt atgaaagcac ttatatttca aaagaagatt cttcgtttgc cgagccgacc
     3781 agcgtagatg tcgatctgga gccggttcag ctgggtaagg cctacaggat gaaagatatt
     3841 tattatgaaa gcaattctgc tgatcttaca gctgaatcga aaaaggtgct ggatgaattt
     3901 attgaatttc tgaatgaaaa cccgaatatt aaagtcaaga ttcagggtca tactgacaat
     3961 atcggaggtg atgcatttaa tcttactctt tcagaaaacc gtgctcacag tgtgtatgag
     4021 tatcttgtat cgagaggtgt ttcttcgtcg agacttagct acaaaggttt tggcgaaaca
     4081 caaccggttg atacgaatga taccgaagcc ggtcgcgcca tgaaccgccg tacggagttt
     4141 cttgttactg gcatgtaagg ttgctgatag tgtcgcgcag agggagttta gtccgacgaa
     4201 ggcgaacctg agacgcagag agattttaaa aattcatacc gtagaattcg tagaggtgca
     4261 gagagtactt tcttacgcct aacgcctgat gcctcacgcc tttagaaact ttacaaacta
     4321 catagggtct gctgaaaaag tctggggcaa attaaaaact tggggagaaa gtgtattttt
     4381 gggttcaaaa atacattgaa tggaaaagga tttactgcgg ttggttttac cagagaaatt
     4441 gctggatcat ttcactataa cagaagtgaa agaagtgttt gataatgcca caaaaaagat
     4501 gtgttatcac attttattgg aggagcacaa taagataccg gaaggatata atgaaaagga
     4561 atacgaatcg aaaggatttt atccggcgaa aacggtacag gatttcccga tacggggcaa
     4621 ggccgtgttt ctgataataa cgcgacgcag atggcggcac aaagagcgaa aggaagaagt
     4681 gatacacagc gattatacgt ttatagcaga gggctcgagg attacaaaag agttgtctga
     4741 ttttttaaaa tatacaggta gatacgcgcg aggacacgat aagtaatatc agcagctatt
     4801 ttggagtaaa tgctgatact ttgcgcaggc aatacaagaa gcacggtagc ggttttcagg
     4861 aatgggatca gaaaactcat gctactcaat acatgatatt tccagagaac attggagaat
     4921 atataagcat tgatgaggtc tcgctatcgc aaggagagct ttacaccttt gttacaaata
     4981 aaaacggaca tggcagaaat gggtcactta tagccagcat caaaggaact ctttccaaag
     5041 atatcatcag tgtattagaa aagctgcctt tagaaaaacg tgagatggtg aaggaagtga
     5101 cgctggatat ggcaaaaaat atggaatcat cagcaaggca aatgtttcca aaaagcaaat
     5161 tggttacaga tcgctttcat gttgtaaaat tagtcattga tgcattgcag catgttcgta
     5221 taaatcttag atggactgag ttggacaagg aaaacaatgc aatagctgag gcaaaaaaac
     5281 ttaaccaaaa atacgaaccg gtagtgtttg aaaatgagga cacgccaaaa caaattctgg
     5341 ctagaagcag gtatattctg accagaaaac ctacggactg gaccgactct caaaaacaaa
     5401 gggctgcaat tcttttcaga gaatatccgc tcttgaaaac agcctatgag cacaccatgg
     5461 agtttaggtc catatatgaa tgtaaaattg aaaaatatgc aaagaaaagg ttccttgact
     5521 ggattgaaaa aacccattct ctgctgatta aagagttcta tacggttgca aataccgtca
     5581 agattaattt cgacaacatc ttaaacttct ttatcaatag aagcacaaat gcttttgcag
     5641 agtctttcaa ttccaaaatc aaattattca gagctaacca aagaggtgtt gttgatacaa
     5701 gttttttcct cttcagattg cacaaattat ttgcttagtc cccaaatatt ttatgtgtcc
     5761 caaaagtctg aggcgcgcca gatttgaggc gacaagacga agatgtatag taatac
//
LOCUS       DAOPKE010000139         5529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_15873_length_5529_cov_11.836865, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000139 DAOPKE010000000
VERSION     DAOPKE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15873_length_5529_cov_11.836865"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(828..901)
                     /locus_tag="PKY63_12045"
     tRNA            complement(828..901)
                     /locus_tag="PKY63_12045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(865..867),aa:Pro,seq:ggg)
     gene            1104..3467
                     /locus_tag="PKY63_12050"
     CDS             1104..3467
                     /locus_tag="PKY63_12050"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPF01391.1"
                     /translation="MRPTILLLLLLFVLNVHGQKFTISGHVKDSATGESLIGASVYLK
                     ELMKGGTTNTYGFYSITADAGNYTLVSSFIGYKEYRKNISLNKNIVMDIELSSQMITT
                     KEVVITGDRPDENLQNTEMGTFDIPVEKIKSIPVLFGEVDVLKTITLTPGVQSGGEGN
                     SAFYVRGGGPDQNLVLLDEAVVYNASHLFGFFSVFNADALQHIELTKSGMPANYGGRL
                     ASVLDISMKDGNMKKYQVDGGLGLISSRLTVQGPIKKDTSSFIISGRRTYADLVMRPF
                     IRKTSDFKHTGYYFYDLNAKINYKISDKDRIFASAYFGRDLFNMDIENDDLKMNMYWG
                     NGTVTARWNHLFSQKLFSNASFIFSDYRFEFGTTMDMYDISLLSGVNDYNARLDFTYL
                     PASSHVLKFGADYIFHVFTPNNASARSGDVDLMVGDAVKLYSHEASVYINDEFSVGKK
                     LKFNVGLRGSFFAHVGPFDRYIKDSITSQVIDTIKYGNGELVKPYWHLEPRASVRYTL
                     NSKSSLKASFTQNYQYIHLASPTTVSMPTDIWFPSTEIVKPQFGTQYSLGYYRNLKDN
                     LIETSVEVYYKLMKNQIEYAEGALIEDNINNNTDNNFVFGTGQAYGIEFFINKKFGST
                     TGWIGYTLARSTRQFDDINNGDVYPAKYDRRHDISFVLTHQITEQITISGVWVYATGN
                     SATMPVSRYIIGGNVVSEYGPRNGFRMPAYHRADLSLTWYPKSKKKTRKIEESWNFSI
                     YNLYNRKNPYFIYFDVEGDILAGSASVTAKQVSIFSILPSITWNFKF"
     gene            3471..4352
                     /locus_tag="PKY63_12055"
     CDS             3471..4352
                     /locus_tag="PKY63_12055"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPF01392.1"
                     /translation="MKKFLFVIIALSFFASCEKELDLDLPQPDAKLVVEGWVESGEYP
                     VVIVTRNSSYFAPIDTNFLMDSLFITDALVIVSDGFQNDTLQPMLDVGAYQNGAWPIF
                     YYKGSKILGAEHGLYSLYIEAEGEVITGQAEIPSLVAFDSLWWEAEAGTGDSLGYIHA
                     MVTDNGAEKNYYRIFSKRLGRDYNFIPIMESIYDDIYFNGLTFEYEIMRGELNYEDEE
                     LFEDPEFGNYTKGDTVVVKLSAINKAHYDFWRTLEEDVMAGGNPFTNPVTIRHTVDGA
                     LGVFGGYGSVCDTIVIQ"
     gene            4366..5316
                     /gene="trxB"
                     /locus_tag="PKY63_12060"
     CDS             4366..5316
                     /gene="trxB"
                     /locus_tag="PKY63_12060"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPF01393.1"
                     /translation="MNDREKRKCVIIGSGPAGYTAAIYTSRADLKPLLYEGMQPGGQL
                     TITTEVENFPGYPQGVTGPVLMEDLKKQAERFGTEIRFGFIDKVDFSQSPHILTDDSG
                     KEIEAETVIIATGASARWLGMPSEQEYNGYGVSACATCDGFFYKGKTVAVIGGGDTAT
                     EEAAYLSNLCSKVYLIHRRDELRASKAMQQKVFNTPNIEMVWNAIPKEIVGETKGFAK
                     AVNGIIVSDSKTGEDRTIPVDGVFIAIGHTPNTDIFKGQVELHDNGYIKTIPGTTQTN
                     IPGVFAAGDVQDLHYRQAITAAGSGCMAAIEAERYLNMKE"
BASE COUNT         1576 a         1164 c         1219 g         1570 t
ORIGIN      
        1 gcagaaatat cgcttgcagc aacgtttttt gattttttca gcagacccta aataaatcaa
       61 ttaccataaa taggatagaa atattaaaca gaccaaggga ttttgatcat agatgaatgc
      121 agaaattacg tagaaatttg ttggtattta tgtgttttgg gtgagataga gcctgtgaaa
      181 acaacagaga tagtctcaaa acagcttatt gttacgacat tccaccgttc cggcaaacat
      241 ctgagttgaa aaatgatatt gctgccggat catggaatcg tcgtaacaat agcaaaaaca
      301 acgattccat atcctttcgc tcctttgtcg ctatcggacg atggaatatt gtttttgaag
      361 aagacgttgt tttgagacat tctcttgtgt aacaaaaaca ggttgccatt gctgacaacc
      421 tgtctgtcgg agttccgctt ccagcggact aaactccccg actcgaattt aacgttggaa
      481 tcaatggctt ccccagccaa gggagtttat tccgccggag gcggaactaa cccactgcgc
      541 tactattctg gtcttctaaa aatctgatta caccacggct tttgattttt ctttccattg
      601 ccacagcttc agttctcgtt gcaaaatcag ttttaaaaac gcaagtccaa tcatcagcaa
      661 aacaggttga tttgcatttt aagcgcagat gttccctgaa tctgttttca aaattgccgc
      721 tttgcccgat gtaatattgc cgggcttttt cagaatacaa aatgtatgtc gtaaacatgt
      781 taatttaatt gaacaaaaac aggttgccat tgctgacaac ctgtctgtcg gagcggtccg
      841 actcgaacgg acgaccactt ggcccccagc caagtacgct aaccaactgc gctacgcccc
      901 gattttggag tgcaaagata caaaaatttt gattctgtaa tttttttaat cggacagcag
      961 gcaattttgt gaaagtcagc tatcataaat ttaaaaacaa atcttttttt tcgacaaaat
     1021 gtaacttttt caggtaataa tatgtctatt tgtcagttat tttgcatggc acaattaatg
     1081 actacgctta ccaacttttt tgtatgagac caactatttt attgctactc ttgttgtttg
     1141 tactaaatgt acatggacag aaattcacta tcagcgggca cgtaaaggat tcggctaccg
     1201 gtgaatcact catcggagcc agcgtttatc tgaaggagct gatgaaaggc ggaaccacca
     1261 atacttatgg cttttattcc atcacggccg atgctggtaa ttacacattg gtaagctctt
     1321 ttattggata taaagaatat agaaaaaaca tttcgctcaa taaaaatatc gtcatggata
     1381 ttgagctttc ttcgcagatg attactacaa aagaagttgt tattaccggc gaccgtcccg
     1441 acgaaaatct gcagaatact gaaatgggaa cttttgatat tcctgttgaa aaaattaaat
     1501 caatccctgt tttatttggt gaagtggatg tgttgaaaac aatcactctt acacctggcg
     1561 tgcaatcggg tggtgaaggc aacagcgcat tttatgtcag gggtggaggt cccgatcaga
     1621 atctcgtttt acttgacgaa gcggttgtgt ataatgccag tcatcttttt gggtttttca
     1681 gcgttttcaa tgccgatgca ctgcagcata tcgaactgac aaaatcgggg atgccagcca
     1741 attatggcgg acgtctggct tcggtgcttg atatcagtat gaaagacggc aacatgaaaa
     1801 aatatcaggt cgatggtggt cttgggctta tttcgtcgcg tcttaccgta caaggtccca
     1861 tcaaaaaaga cacatcgtcc tttattatca gcggacgtag aacctatgcc gatttggtca
     1921 tgcgcccttt tatccgtaaa accagtgatt ttaaacacac cgggtattat ttctacgacc
     1981 tcaatgcaaa gattaattat aagatcagcg ataaagaccg catttttgcc agtgcctatt
     2041 ttggccgcga ccttttcaat atggacatcg aaaatgatga tctgaaaatg aatatgtatt
     2101 ggggcaatgg cacggtaacc gcaagatgga atcatctgtt cagccagaaa ctcttttcaa
     2161 acgcttcttt tatttttagt gattaccgct tcgaattcgg tactaccatg gacatgtatg
     2221 atatcagtct tttgtcgggc gtcaatgact acaatgcgcg gcttgatttt acgtatttgc
     2281 cggcctcgag tcatgttttg aaattcggtg ctgactatat ctttcatgta tttactccca
     2341 acaatgcttc ggcccgctcg ggcgatgtgg atctgatggt tggtgatgct gttaaactgt
     2401 attcgcacga agcttcagtt tacattaacg atgaattcag tgtgggcaaa aaattgaaat
     2461 tcaatgttgg tctccgtgga agcttttttg cgcacgtcgg accctttgac cgctatatta
     2521 aagactccat caccagtcag gttatcgaca ccatcaagta tggtaatggg gaattagtaa
     2581 aaccctactg gcatctcgaa ccacgcgcaa gtgtccgcta cacacttaat tcaaaaagct
     2641 ctttaaaggc atccttcact caaaactatc aatacattca tctggcttcg ccaaccactg
     2701 tgagcatgcc gaccgacata tggttcccaa gtactgaaat tgtaaaacca caatttggca
     2761 cacagtattc gcttggttat tacagaaatc tcaaggataa tctgattgaa acgagtgttg
     2821 aagtatatta caaactcatg aaaaatcaga ttgaatatgc cgaaggagct ctgattgaag
     2881 acaacataaa caataacact gataataatt ttgttttcgg aaccggacag gcgtatggta
     2941 ttgagttttt catcaataaa aaattcggca gcacaacagg atggattgga tacacgcttg
     3001 cccgcagcac cagacagttt gatgacatca acaatgggga tgtctatccg gccaaatacg
     3061 atcgacggca cgatatatca tttgtattga cgcatcagat tacagagcag atcaccattt
     3121 cgggtgtgtg ggtctatgct accggaaata gcgcaactat gccagtttca cgttatatta
     3181 tcggtggaaa tgtcgttagt gaatatggcc ccagaaacgg tttccgcatg ccggcctatc
     3241 atcgtgccga cctgagtctg acctggtatc cgaaaagcaa aaagaaaaca cgtaagattg
     3301 aagaaagctg gaatttttcg atttataatt tatacaatcg caaaaatcct tatttcattt
     3361 acttcgatgt tgagggcgat atcctggctg gaagcgcatc tgttactgca aagcaggttt
     3421 ccattttctc cattcttcca tcaattacat ggaattttaa gttctgagtt atgaaaaagt
     3481 ttttgtttgt tatcattgcc ctttcctttt ttgcatcgtg cgaaaaggaa cttgatttag
     3541 atctgccgca gcctgatgca aagctggttg tggaaggatg ggttgaaagc ggagagtatc
     3601 cggtagttat cgtaacccgc aatagcagct attttgcgcc catcgatacg aattttttaa
     3661 tggactcatt gtttatcacg gacgcgcttg ttattgtttc tgatggtttt caaaacgata
     3721 ctttgcagcc gatgcttgat gtgggcgcct atcaaaatgg tgcctggccg attttttatt
     3781 ataaaggatc aaaaattctt ggcgctgagc atggattgta ttctctgtat attgaagccg
     3841 aaggcgaagt aatcaccggg caggcagaga ttccgtcact ggttgcattc gacagtttat
     3901 ggtgggaagc tgaagccgga accggcgact cgctcggtta tattcatgcc atggtgacgg
     3961 ataacggtgc cgagaaaaac tattaccgca ttttttcaaa gcgccttggc cgtgactaca
     4021 attttatccc tatcatggag agtatttacg atgatattta tttcaacgga cttacgtttg
     4081 aatacgaaat catgcgcgga gagctgaatt acgaggatga agaattgttt gaagatcctg
     4141 aatttggcaa ttatacaaaa ggtgacactg tagttgtgaa actttctgcc atcaacaaag
     4201 ctcattacga tttctggcga acactcgaag aagatgtaat ggccggcgga aatcccttta
     4261 caaacccggt gaccatccgg cacaccgttg atggtgcgtt gggcgtattt ggcggctacg
     4321 gcagtgtttg tgacacaatt gtaattcaat aaaacgcaaa atataatgaa cgacagagaa
     4381 aaaagaaaat gtgtgataat cggctccggc cctgcaggat acactgcagc catttatacc
     4441 tccagagctg atctgaaacc gcttttatac gaaggcatgc agccgggcgg acaattgaca
     4501 attacaaccg aagtcgagaa tttcccgggc tatccgcaag gtgtgaccgg acctgtgctg
     4561 atggaagacc tgaaaaaaca ggcagagcga tttggcactg aaatacgttt tggatttatt
     4621 gacaaggtcg atttttcgca aagtcctcat atccttaccg atgacagcgg aaaagaaatt
     4681 gaagccgaaa cggtgattat tgctaccggc gcttcggcgc gctggcttgg tatgccgagc
     4741 gagcaggaat acaacggcta tggcgtatcg gcctgtgcta cctgcgacgg atttttctac
     4801 aaagggaaaa ccgttgccgt catcggagga ggggataccg ccaccgaaga agctgcctac
     4861 ttatcgaacc tttgttccaa agtatatctg attcatcgcc gcgatgagct gcgcgcctcg
     4921 aaagccatgc agcaaaaggt gttcaatacg ccgaatatcg aaatggtctg gaatgccatt
     4981 ccgaaagaaa ttgttggtga gaccaaaggc tttgcaaaag cggtcaacgg cattatcgtt
     5041 tccgattcca aaaccggcga agaccgcacc attcctgtag atggcgtatt cattgccatc
     5101 gggcacaccc ccaacaccga tattttcaaa ggtcaggttg aattgcacga caacggatat
     5161 atcaaaacca ttcctggcac cacgcagacc aatattccgg gcgtttttgc agccggcgat
     5221 gttcaggatc tgcactaccg ccaggccatt actgctgccg gttccggctg catggccgcc
     5281 atcgaggccg aacggtatct gaatatgaaa gagtaatgaa ctctgaacag cgaacaagga
     5341 agtgcgaagt ttgaagtaaa atccactttc ttgttctttt ttatttttcc cgacttcgcc
     5401 actgggacac ccatgttttt tctgaaattt ttgaggaggg gtattcaatg agacaaatag
     5461 aagcgagtaa atgtttatta tgaatttttc ctgacccgcc agctggcggg tcaaacaata
     5521 atagcccgg
//
LOCUS       DAOPKE010000140         5489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_16028_length_5489_cov_12.654398, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000140 DAOPKE010000000
VERSION     DAOPKE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16028_length_5489_cov_12.654398"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1409)
                     /locus_tag="PKY63_12065"
     CDS             complement(195..1409)
                     /locus_tag="PKY63_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01394.1"
                     /translation="MIKKSTLAEVVEQQHLLQLNTDIGYRRDLLSEININLNDFALII
                     SGIRRCGKSTLLKQLMEKTKKKYFFLNFDTARLYTFASDDFALLDELIAEKDYKILFF
                     DEIQVVDGWELFVRQKLDEGYRVVLTGSNATLLSKELGTKLTGRHITKELFPFSYGEF
                     CAFKTLKTGAASFSDYLNSGGFPAFLKEQNIDLHSALLDDIIYRDVAVRHNIKDVHSL
                     KHLVLFLASNVGNLISATKLKQLIGIKSTATILEYLSFFEQSYLIQLVPKFSYSYKVQ
                     VANPRKVYFIDSGLQNAVTGSFNNDNGRKLENVVFWELRRLQKELFYYNENGKECDFV
                     VCRNKAVEQVMQVCYELNTDNERREIDGLIDAMDFFKTAEGLILTYAQEDVISHGNKR
                     IKVIPAFRYFGS"
     gene            complement(1819..2727)
                     /locus_tag="PKY63_12070"
     CDS             complement(1819..2727)
                     /locus_tag="PKY63_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01395.1"
                     /translation="MKTDFEKLGLITHSKLTRATQLLFGTHHTAIHIGRFKAEDTIID
                     DLIIRSPLILAVDEAMNFIKRNISISYEFTGELKRTEHWQYPLQAIRELLLNAVVHKD
                     YTNPTDVIVKIFDDRIEISNPGRLINGLTLADLGTDHYQPRHRNKLLAEIFYLTGDIE
                     KYGTGFIRLRKLLSDSSDINFQILDINGFIKVILSTSKSEGRTPIGTPIGTPIGGAIG
                     GQIGGAIGGAIGGAIGGAILTERQLEIVALMKENNQITYRKIAKVLKINDSAVLKHIK
                     ALKLNGTIERIGGTRGYWLIKTIRSD"
     gene            complement(2770..3252)
                     /locus_tag="PKY63_12075"
     CDS             complement(2770..3252)
                     /locus_tag="PKY63_12075"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPF01396.1"
                     /translation="METIDKIKDLIFQGESEILELKSSFTKDVILTIVAFSNTKGGTI
                     IIGCNDKREITGVDLSDESIQKWTNEIKQNTEPAVFPSFEVFKVDGKSIVTIIVDEFP
                     IKPVSYRNRFYCRRQNSNHLLSTDEIVELRFISLNYSFDSFPVPDKIDQLDKGALAYF
                     "
     gene            complement(3472..4410)
                     /locus_tag="PKY63_12080"
     CDS             complement(3472..4410)
                     /locus_tag="PKY63_12080"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF01397.1"
                     /translation="MNHYNNQVGLLLRLLPFIAREQIFALHGGSAINLFHFEMTRLSV
                     DIDLTYIPFSDRDNDLSQIQLGLERIKMRLLNAIPGINVREPLQNQNEYKLFCSLNGA
                     EVKIEVNTINRGVFQPVEMRLLCSAAQNAFSTFCEMPVVSVGQLFGGKIVAALDRQHP
                     RDLFDVNNILKTFGWSKEISDGFVFCLLSSKRPIHELLNPSFIDQHSCFQSQFSGMTN
                     ETFSYEMFETVRTKLIETILSNISPADSDFIISFADAKPNWEFKNYSRFPGIQWKLQN
                     LQKLKSQNPDKFQSQHTELKRILFSSLNSPDIQQLC"
     gene            complement(4407..5174)
                     /locus_tag="PKY63_12085"
     CDS             complement(4407..5174)
                     /locus_tag="PKY63_12085"
                     /inference="COORDINATES: protein motif:HMM:NF022898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HPF01398.1"
                     /translation="MSTNNETKINRLLQMIPTGVVLASSWLTQQGYSRDLQQRYIKGG
                     WLSPIGRGAFIRKGDEVDVFGALYCIQTQLKKNMHIGGRSALGLQGLSHYVEIYQKEY
                     ILFAHRGTRVPQWFLNNVWNSKPQLIFTEILPPESGLIAYNTGSFPILISSPARALLE
                     CLYLAPARFDLLEAYQIMEGLASLQPKPVQDLLEQCSSVKTVRLFLYLAEKTAHPWFR
                     HIDASKLKLGNGKRSIVENGIYVPKYKITIPSDIATL"
BASE COUNT         1651 a         1093 c         1081 g         1664 t
ORIGIN      
        1 gaaatcagaa atacgagata tgaaatacga gatgttttat tattcgcttc gcgaagggag
       61 atttatgtgg gcgcgttgcg ctgaacttta agaaatacga aatacgaaat atgaaatgcg
      121 agagaggcga caggcgttag gtcccgcctt cgtcgggata aactccaggc gttaggcatg
      181 cggcatgctt gtccttagct gccaaaatac cgaaatgccg gaataacctt aatacgtttg
      241 ttgccatgag atatcacatc ttcctgagca tacgtcaaaa taaggccttc agcggtttta
      301 aaaaaatcca ttgcatcaat aaggccatcg atttcacgtc tttcattgtc tgtattaagt
      361 tcatagcaaa cctgcattac ttgttcaaca gctttgttgc ggcaaactac aaaatcacat
      421 tcttttccat tttcgttata ataaaaaagt tctttctgca gccgtcttaa ttcccaaaaa
      481 acaacatttt cgagttttcg tccattgtca ttattaaacg atcctgttac tgcattttgc
      541 aaaccactgt cgataaaata caccttgcgg ggatttgcaa cctgaacttt gtacgaatac
      601 gagaatttgg gaaccaactg aatcaaatac gattgctcaa aaaaactgag atattcgagg
      661 atagtggccg tactttttat tccaatcaac tgtttgagtt ttgtggccga aatcagattt
      721 ccgacattcg atgccagaaa taaaaccaaa tgtttaagcg aatgaacatc ctttatgtta
      781 tgacggacag ccacatcgcg gtaaataata tcgtcgagca atgctgagtg cagatctata
      841 ttctgttctt taagaaaggc cggaaaaccg ccggaattta aataatcgga aaacgaggcg
      901 gccccggttt tcagcgtttt aaatgcacag aattcaccgt atgaaaaagg gaaaagctct
      961 tttgttatat gacgtccggt cagtttggtc cctaattctt tgctcagcaa tgttgcgttt
     1021 gacccggtaa gaaccacccg atagccttca tccaatttct gtctgacaaa taattcccaa
     1081 ccatcgacca cctgaatttc gtcgaaaaac aaaattttgt aatccttttc ggcaatgagt
     1141 tcatccaaca atgcgaagtc atcagaagca aatgtgtata atctggctgt gtcaaaattc
     1201 aggaaaaaat attttttctt tgttttctcc atcaactgct taagcaaagt gctttttccg
     1261 cagcgtctga taccagaaat aatcaatgcg aaatcgttca gattgatatt tatctcagaa
     1321 agcaaatccc ggcgataacc tatatccgta tttaactgca gcaaatgctg ttgctcaaca
     1381 acttcagcca gtgtcgattt cttaatcatt ggggtgaatt attaccaaca caaagataag
     1441 tgtttattct gaatgaacaa tagtgtttat ttgcaatgaa caatttatgt aaggaaatta
     1501 acgctaaaat aagaatttga aacacgagtt cagaaatacg aaatatgaaa tacgaaatac
     1561 gaaatacgag atgcgaaata tgaaatacga gatacgaaat acgagatgcg aaactctttt
     1621 agcggtgaac ggtttgaagt ggtcagttgt gagtggtcag ttgtgagtaa aatctaactg
     1681 gctactggct actggagaga ggtttcattg gttcccttcg ggaaggattg atgtgggcgc
     1741 ggagtttatc ccgctggaag cggggtgctg agcgggggcg gtttaacata aatgttggat
     1801 attttagcag gcaaggtatt aatcgctcct gatggtttta ataagccagt agccgcgggt
     1861 tcctccaatt cgttcgattg tgccattcag cttcagtgct ttgatatgtt ttagcacagc
     1921 cgaatcgttg attttcagca ccttggcaat tttcctatat gtaatttgat tgttttcttt
     1981 cattaatgca acaatttcca actggcgttc tgtaaggatt gcaccaccta ttgcaccacc
     2041 tattgcacca ccaattgcac cacctatttg accacctatt gcaccaccaa ttggggtacc
     2101 aattggggta ccgattgggg tacgaccttc actttttgaa gtggatagaa taacctttat
     2161 gaagccgttg atatccagta tctgaaaatt aatatcagaa gaatcgctca acaattttcg
     2221 caaacggatg aaaccggtgc cgtatttttc aatatcgccg gtaaggtaga aaatctcagc
     2281 cagcagctta tttctgtgtc ttggttggta atggtctgtt cccaaatctg caagagtcaa
     2341 tccatttatc aaccttccgg gattgctgat ttcaatacgg tcatcaaaaa tctttacaat
     2401 aacatctgtt ggattagtgt agtctttgtg cactacggca ttcagtaata attccctaat
     2461 ggcttgcaaa ggatattgcc agtgttcagt tcttttcaat tcaccggtga actcatagct
     2521 gatcgagata tttcttttta taaaattcat ggcctcatcc accgctaata tcagcggact
     2581 acggataatc aaatcatcaa tgatcgtgtc ctcggcttta aaccggccaa tgtgtatggc
     2641 tgtatgatga gtgccaaaca ataattgggt tgcccttgtc agttttgaat gagtgattaa
     2701 tccgagtttt tcaaaatcag ttttcaagtc agcacccgga ttatagcgtc cggtttgaga
     2761 aacaattttt taaaaataag ccagcgcgcc tttatctaac tgatcaattt tatcgggtac
     2821 aggaaatgag tcaaaggaat aatttagact aataaaccgc aattcaacaa tctcgtcggt
     2881 cgacaataaa tgattggaat tttgtctgcg acaataaaaa cgatttctgt aagacaccgg
     2941 ctttatggga aattcgtcaa caataattgt cacaattgat tttccgtcca ctttaaacac
     3001 ttcaaatgat ggaaataccg ccggttcggt gttttgctta atttcgtttg tccatttctg
     3061 aatgctttca tcagacaggt caactccggt aatttcccgt ttatcgttac aaccaataat
     3121 gatagtacca ccttttgtat tcgaaaatgc aacaatagtc aatatcacat ctttggtgaa
     3181 agatgatttt aattccaata tttcagattc gccctgaaat attaaatctt ttattttgtc
     3241 tattgtttcc aatcagagaa atttataatt gggctgcgct aagttatgag ctgtaataaa
     3301 tacgaaagac gaaagacgaa atttgaaatg cgaacgaatg tggagatgtg aaaatgtgtg
     3361 gcgctcacgt gaaatcacgt aaaacgtgac aagctgtgac aagttcgcgc gggagattta
     3421 ttatggcgcg gagttcatcc cgctgaaagc ggggcacgac acgcgggcgg tttaacataa
     3481 ttgttggata tcaggactgt ttaatgaaga aaataatatt cgtttcaatt cagtatgctg
     3541 actctgaaac ttatccgggt tttgactttt tagcttttgc aaattttgca gcttccactg
     3601 aattccggga aaccggctgt aatttttgaa ttcccaattc ggtttcgcat cagcaaagct
     3661 tattataaaa tctgaatcgg ctggagaaat atttgacaaa atggtttcaa tcaacttcgt
     3721 cctaacggtt tcaaacatct cgtaagaaaa agtttcattg gtcatgccgc tgaactggct
     3781 ttgaaaacag gaatgttgat caataaaaga aggattcagt aattcatgaa ttggcctttt
     3841 gctacttaat aaacaaaaca caaatccatc cgaaatttcc ttactccatc cgaaagtttt
     3901 cagaatatta tttacatcaa ataaatcgcg cggatgctgt cggtcgaggg ctgccactat
     3961 ttttccacca aacagctgtc cgacagaaac caccggcatt tcacagaaag tactgaatgc
     4021 attttgagca gcactgcata aaagcctcat ttcgacaggt tgaaacacac ctctgttaat
     4081 tgtgttcacc tcaattttca cttccgcacc attcaaggaa caaaacaatt tgtattcgtt
     4141 ttgattttgt aatggctcac gtacattaat tccgggaatc gcattcagca ggcgcatttt
     4201 tatacgctcc aaaccaagtt gaatttgaga caaatcattg tctcgatccg aaaacggaat
     4261 ataagtcaag tcaatatcaa ctgataaccg ggtcatttca aagtgaaaaa gattgatggc
     4321 agacccaccg tgtaatgcaa aaatctgttc acgtgcaatg aaaggcagta aacgcagcag
     4381 taatccaacc tgattattat aatggttcac aacgtagcaa tatcggacgg aatggttatt
     4441 ttatatttgg gcacatagat gccgttttca acaatacttc ttttgccatt tcccagcttc
     4501 agtttggatg catcaatatg tctgaaccag ggatgagcag ttttttcggc gagatataaa
     4561 aacaatcgta ctgttttcac cgaggagcat tgttcgagta aatcctgtac tggtttcggt
     4621 tgcaacgatg caagtccttc cataatctga taagcttcaa gtaaatcaaa gcgggcgggc
     4681 gcaagataca ggcactcaag aagagccctt gcaggcgaag aaatcaaaat aggaaatgag
     4741 ccggtgttgt aggcaatcag ccctgattcc gggggcagta tttcggtaaa tatcagttgc
     4801 ggtttgctgt tccacacgtt attcaaaaac cattgcggca cccgggttcc ccggtgggca
     4861 aataggatat attccttctg atagatttcg acatagtgcg acaatccttg taatcccaat
     4921 gcactccgcc ctcctatgtg catatttttt ttgagttgcg tctgtataca atacaatgct
     4981 ccaaacacat caacctcatc gcccttgcga ataaatgcgc ctctgccaat ggggcttaac
     5041 caaccgcctt tgatgtatcg ctgctggagg tctctggaat atccctgctg tgttagccat
     5101 gaactcgcca ggacaactcc cgtgggaatc atttgtaaca aacggtttat ttttgtttca
     5161 ttattagtac tcatagtaca aatatacgaa catatttaaa ctaaatgata ttgaatgccg
     5221 ctttttaata aaaggaaggt aaatacctga gacaaatatg ttccgatgaa tcaaaatatt
     5281 ttgacttttt tgaacgtctt gactaattta aagtttacca cgaattggta tcatgagccg
     5341 gattttgtcg atgaaagtct tatacggatt cgtttctaaa ccccttataa atgtgtgggt
     5401 taaaattgcc attgtgcaaa tttcagactt ttcgttgtgc aaatttcaga cttttttatg
     5461 gttgtaacca caattattca aaattgttt
//
LOCUS       DAOPKE010000141         5445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_16205_length_5445_cov_7.236920, whole genome shotgun sequence.
ACCESSION   DAOPKE010000141 DAOPKE010000000
VERSION     DAOPKE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16205_length_5445_cov_7.236920"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PKY63_12090"
     CDS             <1..1247
                     /locus_tag="PKY63_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF01399.1"
                     /translation="NQPSTINFTGSNNPGVTYTYDFNGGTVISGSGSGPYEVSWPAAG
                     TYTVTLTTSEGSCTNTETQTVTVNSNITVSISPAAPSVCPDSSITLTASGGATYSWAA
                     DPTLNSDSTALVTATPTTTTTYTVTGTSIEGCTGSASVTVDIYPEPTISVTPVPSNGC
                     IPHVVDFSASVSPAATAYSWDFGDPASGSQNNSTSDSPIHVFSNAGQYDISLSVISVH
                     GCPGTATFPSMITAYDVPVAAFLTDSTTLNLMNPVVNFTDMSTGALNWYWDFGEPSSY
                     SNFSNLTNPTHTYGSTGEFIVWMVVDNGYCSDSTYTIINVVQDIAFYMPNAFSPYNED
                     GVNDIFAPSGIGAGMGDNTFAMYIYDRWGKLVFSSKDFFSGWDGRYDGEFVQPGVYTY
                     FVEVQYADKLWRKFWGKVTLVK"
     gene            complement(1292..3175)
                     /locus_tag="PKY63_12095"
     CDS             complement(1292..3175)
                     /locus_tag="PKY63_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01400.1"
                     /translation="MNIFSPRRFSRFLLVIAIFSPSLLFSQQSSDHVRKEKTEIRNLL
                     DSASMYAENDPAISEPMAIAAYQLAESIGDTLSMSEALRLYAVGSQFLHGRDTTEHVF
                     YQSMELAGQSFSCQRAKSILWYADFLRKSDGAEKALSLLKPNAGKLLSCGMMYEFMYN
                     IYVIGLCYEKLGQDTNAVFVFNNLLKTAKYQNDYFFQFKAYNSLLIVFLKLNKNPEYL
                     FQAMMLSAEKSKNPDFQAVGWNNIAKFYIKSGDTTKAEYCFDKSIIKSLETRNDENIA
                     HISLNISMHYIRRGNFPKAIRLFRMAEERKQYIIQDNWVGVYYGIRARIHMHHGNLSA
                     AEEDITIAMRYFESVNNYPMLIEFNSIAAEIFGALNKFDQAYYYQSQVLKYQQLFDSK
                     KDNSVENNLYYKVLLDLEKAKAKTIVLENENLVKQRRISYLLTSIFVILLVLVVLFIY
                     FLSSRYKRRILNSEQQKETLLAARIRLTAELNTKNKEIASFVLNQAMVNETAGDVITQ
                     LRQLGSKSPRETQKKLYELASVLSTSQNKNIWKEFDHYFSLVNPDFFSRLTQAHSGLT
                     SRDLRYCALISLQLSSKEMALITGMTLQSIHVLRSRLRQKLSVDRDEDLGVYLSQFSE
                     AAV"
     gene            3796..>5445
                     /locus_tag="PKY63_12100"
     CDS             3796..>5445
                     /locus_tag="PKY63_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01401.1"
                     /translation="MKSKLLMIIWGVLISTFSLQAQTTVLSEDFETLPLDLTSSGSGN
                     WERTTLLAAGGTYSDTATAVLSGHSYLTTPSFSTLGNYMVRLEFDQICKIELFDGGYI
                     EYSLDGGTTWIVVTSTYYQGSGSMASNKFTANSYTSTWVASDFDTIPNNTWWKHETFE
                     LGSLVGNQANVMLRFRLSDLNNNGANTHYGWALDNVLITMALSESTPPSITQLPTIWQ
                     DTLYQTGPFEVKAKINDLSGIDTAYVVYTINGGANNYVGMNMLNVDTFAATIPAQAYN
                     TRIDYFVVAVDGSIAANADSSAAKWFYTKKAAPVVIIGTGTSDYGYTPTYGSYNYGWS
                     KLLYTASEIGTSGIIDSLSFKPGNSPANFLMTDQKVFIASIPDSVFADGAMPDTSVMT
                     AVFEGSVTWNGNNFHGIKLTTPFYYNGTDHLLIYWLNRDGSNATGYPEFTYTSTSPDY
                     QAAYKYSNTYNLVFPTSTGSLSTTYRSNIKITFALSTAVHDVAAVEIAEPLITPNPAI
                     GTPYDVKVKIINIGSDTLTSATLNWELDGIVQTPFVWNGTLM"
BASE COUNT         1603 a         1255 c         1024 g         1563 t
ORIGIN      
        1 tcaatcaacc gtcaaccatc aatttcacgg gttcaaacaa tcccggtgtt acttatactt
       61 atgattttaa cggtggaaca gtgatttccg gctccggatc aggtccctac gaggtttcgt
      121 ggcctgctgc cggcacttat actgttacac ttacaacttc agaaggatcc tgcaccaaca
      181 ccgaaaccca aactgtaacc gtcaattcaa acatcactgt atcgatttcg ccggctgcac
      241 cttcggtgtg ccccgacagc agcataactc tcacagcttc aggtggagct acctattcat
      301 gggcagcaga ccccaccctc aactcagaca gtacagcttt ggttaccgcc actccaacca
      361 ctacaacaac ctataccgtt accggaacca gcattgaagg gtgtaccggc tctgccagtg
      421 taaccgtcga tatttatccc gaacccacca tttctgttac tccggttcct tccaatggtt
      481 gtattccgca tgtggttgat ttcagcgcat ccgtttcgcc agctgccact gcctatagct
      541 gggactttgg agacccagct tcaggatctc agaacaacag cacttccgat tctcccattc
      601 atgtgttttc gaatgcagga caatatgata tttccctcag tgttatatcg gttcacggat
      661 gccccggcac agccactttc ccatctatga taaccgctta cgatgtgcct gttgcagcat
      721 ttcttacaga ctcaacaaca ctgaacctga tgaaccctgt agtcaacttt accgatatgt
      781 caaccggtgc gctcaactgg tactgggatt ttggcgaacc ctcctcttat tcgaatttct
      841 cgaatctgac aaatccaacc catacctatg gatcaaccgg cgaattcatt gtctggatgg
      901 tagtagataa tggctactgt tctgattcaa cctacaccat tatcaatgtt gttcaggata
      961 tagctttcta catgcccaat gccttttcgc catacaacga agacggggtc aacgacattt
     1021 ttgctccatc agggattggt gcgggaatgg gtgataatac ctttgccatg tatatttacg
     1081 atcgttgggg gaaactggtg tttagctcaa aagacttttt ctcgggatgg gatggaagat
     1141 acgatggcga attcgttcag ccgggtgttt acacctattt tgtcgaagta caatatgccg
     1201 acaaactctg gcgaaaattc tggggcaaag taacattagt gaaataagca aaagatattt
     1261 tcaaaagact gttctccgga acggtctttt tttatacagc cgcctctgaa aactgagaca
     1321 gataaacacc cagatcttca tcgcggtcaa cagacaattt ctgacgcaaa cgcgaacgca
     1381 aaacatgaat gctttgcaaa gtcatgcctg ttatcaaagc catttccttt gaactaagct
     1441 gcagactaat caacgcgcaa taccgcagat cgcgcgaagt gagcccgctg tgcgcctgag
     1501 ttaatcttga gaaaaaatcg ggattcacca gcgaaaaata gtgatcgaat tctttccaga
     1561 tatttttgtt ttgagaagtt gacaacaccg atgccagctc atacaatttt ttctgtgtct
     1621 cacgagggct tttactgcca agctgccgca actgagttat cacatcgcca gccgtttcat
     1681 tcaccatggc ctgattcaat acaaaactgg ctatttcctt gttttttgta ttcaactcag
     1741 ctgtaagtct tatacgggcg gcaagcaatg tttctttctg ctgttctgaa ttcagaatac
     1801 gacgtttgta acggctcgac aaaaaataga taaacaaaac aaccaaaacc agcagtataa
     1861 caaatatgga tgtcagcaga taagaaatac gccgctgctt aacgaggttt tcgttttcaa
     1921 gtacaatggt tttagctttg gctttttcga gatcaagcaa aactttataa tataaattgt
     1981 tttcaactga attgtctttt tttgaatcaa acaactgctg atatttcaaa acctgtgatt
     2041 gataataata ggcctgatcg aatttattca gcgctccgaa aatttcagct gcaatggaat
     2101 tgaattcaat cagcatcgga taattattca ccgattcgaa ataacgcatg gcaattgtaa
     2161 tatcttcttc ggcagcagac agattgccat ggtgcatatg aatacgggcc cggattccat
     2221 aataaacacc aacccaatta tcctgaataa tatattgctt gcgctcctcc gccatgcgaa
     2281 acaatcgaat cgcttttggg aaattgcctc gccggatata atgcatactg atattcagac
     2341 taatatgtgc aatattctcg tcatttctgg tctccagcga tttgatgatt gatttgtcaa
     2401 aacaatattc cgcctttgtg gtatcgccac ttttaatgta aaacttggca atattattcc
     2461 acccgacggc ctgaaaatca gggtttttgc ttttttcggc cgaaagcatc atggcctgaa
     2521 aaagatattc agggtttttg ttgagcttga gaaaaacaat cagcaaactg ttataggcct
     2581 tgaactgaaa gaaataatca ttctgatatt ttgctgtttt caataaatta ttgaacacga
     2641 aaaccgcgtt ggtatcctgt cccagttttt cataacacaa tccgataaca tatatattgt
     2701 acatgaattc atacatcata ccacacgata aaagctttcc tgcatttggc ttaagcagtg
     2761 aaagagcttt ctctgcacca tcacttttac gtaaaaaatc ggcataccac aaaatagatt
     2821 ttgcgcgttg acacgaaaaa gactgacctg ccagctccat tgattgataa aaaacatgct
     2881 ccgtcgtatc ccgcccatgc aaaaactgac ttccaactgc ataaagccgc aatgcctccg
     2941 acatggacaa cgtatcaccg atgctttcag ccagctgata agcagctatt gccatgggct
     3001 cggaaatagc aggatcattc tcggcataca tagatgcaga atcaagcaaa ttcctgattt
     3061 cagtcttttc ttttctgaca tgatctgatg actgctgcga aaacaataaa gacggggaaa
     3121 atatggctat gaccaataaa aaccttgaaa accttcgggg gctgaaaatg ttcatacgca
     3181 aaattaatat tttttagacc tttcacaggt gccgaaagat aaaaacaatc aggttttttt
     3241 tcaaaaaaaa caataacatg cattcattaa ttcaatcaat aatgcataaa agaaatccat
     3301 tcagcttaca ttaaacagaa tcaaaacaat atttcagatt cttgcttgcc attcggtttt
     3361 gcatgcatga actctcctga tgtccgattt ttcagaaatc aacacttcgg attctttcag
     3421 attgatctca atcaaggttt acatttactg caacgtcatt tttaaaccct cataaaatta
     3481 ttgtttatcg atattataca ccattttatg taattcataa tagtatttcc ccctaatgac
     3541 atatattcac atttaagaac aatgtattta gcgcttaatt atattttttt ttaaatatat
     3601 aaaattgtat atctctcgca acaaatcagc gtatttaact gatttactta tttttaacag
     3661 tgtcgttaag tatccgataa gcataaaagg tgttaatttc ttaaagacaa tgcaaaggca
     3721 taaaaaaaag gggcttttta atcgtaaatt cgtaagtctt ttttcgtttt gaaaaataag
     3781 aaaaacaaca agcatatgaa atcaaaatta ctgatgatta tttggggggt gctgataagt
     3841 actttctcat tgcaagcaca gacgacagtc ttatctgaag acttcgaaac cctgccgctg
     3901 gatttgacca gctccggcag cggcaactgg gagcgaacaa cgctgctggc tgccggcgga
     3961 acctacagcg ataccgctac agcagtgctt tcaggacaca gttacctgac aacaccctcg
     4021 ttttcgacac tcggaaatta catggtacgc ctcgaattcg accagatttg taaaatagag
     4081 cttttcgacg gcggatatat cgagtattcg ctcgatggcg gcaccacctg gattgttgtt
     4141 accagcacct actatcaggg tagtggcagc atggccagca ataaattcac cgcaaactcc
     4201 tatacctcaa cctgggtggc atctgatttc gatactattc caaacaacac ctggtggaaa
     4261 cacgaaactt ttgaactggg gagtctggtg ggcaatcagg ccaacgtaat gctgcgtttc
     4321 cgtttatcgg accttaacaa taacggagca aatactcatt atggctgggc actcgacaat
     4381 gtgcttatta ccatggcttt atccgaaagc acgcctcctt ctatcactca attgccaacc
     4441 atctggcaag atactcttta tcagaccggc ccgtttgaag tgaaagcaaa aatcaacgac
     4501 ctgtccggta ttgatacggc ctatgtggtt tacactataa acggcggcgc caataactac
     4561 gtgggcatga atatgctgaa tgttgacacc tttgcagcca ccattccagc tcaggcctat
     4621 aatacacgca tcgattattt tgttgttgct gttgacggct ccattgctgc caacgctgac
     4681 tcttctgcgg ccaaatggtt ttatacgaag aaagctgcac ctgtggttat tatcggaacc
     4741 ggaacaagcg attacggcta tactccaacc tatggctctt acaattatgg atggagcaaa
     4801 ttattatata cagcaagcga aattggtact tccggaatta ttgactcatt gtcttttaaa
     4861 ccgggaaatt ctcctgccaa tttcttaatg actgatcaga aagtttttat tgccagtatc
     4921 cccgattctg tatttgcgga cggcgctatg ccagacacat cggtaatgac ggctgtcttt
     4981 gaaggttcag tgacctggaa tggaaataat ttccatggaa ttaaactaac tacacctttt
     5041 tattataatg gaactgatca cctgctgatt tattggctga accgcgatgg atctaatgct
     5101 acaggttatc ctgaatttac atacacatct acttcacccg attatcaggc cgcatataaa
     5161 tacagcaata cctacaacct tgttttccca acatcaacag gttcgttgtc aactacatac
     5221 cgctccaaca ttaaaatcac ttttgcctta agcactgcag tgcatgatgt ggcagcggtt
     5281 gaaatagcag aaccgttaat cactccgaat cctgccatag gaacacctta tgatgttaaa
     5341 gtgaaaatta taaacatcgg atctgacaca ctcacaagcg ccaccctcaa ctgggaactt
     5401 gatggcattg ttcaaacccc gtttgtatgg aacggcacac tgatg
//
LOCUS       DAOPKE010000142         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_16331_length_5413_cov_11.560470, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000142 DAOPKE010000000
VERSION     DAOPKE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_5413_cov_11.560470"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKY63_12105"
     CDS             complement(<1..1520)
                     /locus_tag="PKY63_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01402.1"
                     /translation="MKKTFITSIAVFLSFFALAQSEKDPQASNNSFLNHQSTSCPLKA
                     QWDIQMSFNASDSIGGSTGMAAIALVNSEIWASKWGSDTLIRFDIDGTMIEKFIIAGL
                     TGTRSITTDGTYLYIGNNTNTIYRVDPVTATLAPPHITSASVSTSRFLTYDATLNSGA
                     GGFWTGNFNTDIDAISMSGAVLTTIPAATHTLSGMYGAAVDNYSTGGPFLWVFHQDGA
                     NLSQVSAINLTTGLPTAFTYDMFPPLQTLYGITSGLAGGCFITNQLIPGEISFMGMLQ
                     GTPDNIAFAIELDPTTIVDDIIALSIKPIHGYTQIPESQVFAETFEATFRTEGLNNPD
                     TVFGDVNYYFNGNLLSSEQVQVTNLTFSATDTLVSSAFTPPNGKGTYMIELILSGNAA
                     FTDGNASNDTIRFNIEVTDSVYARDNGVSTGTPYNVSSSDWAYAASMFELFQTDTIEA
                     IWIQLETPVNGDTTFGVIYSMLGGMPSTFVATSDTTIIDSTINAYMLTFSSPVILPA"
     gene            1963..3189
                     /locus_tag="PKY63_12110"
     CDS             1963..3189
                     /locus_tag="PKY63_12110"
                     /inference="COORDINATES: protein motif:HMM:NF026954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPF01403.1"
                     /translation="MKKNVVVSCGNSGPKVRSDCRIEMQITDQGGIQIELKSKVKVLY
                     GNDIIRVTKEVAGFYGIENCQISIEDSGAVTPVLAARLEAAIRQLVDTDKEYLLPVIP
                     QNHYETEKERNRISRLYLPGNSPAMFLNAGIHHPDGLILDLEDAVAPDKKFEAAFMVR
                     NALRNINFYGAEKMVRINQIPRGLDDLKFIVNHGVNLILVPKCESAEQIHMVNDKIAE
                     IATDKNRNIWLMPIIESADGVLRAKEIASAAPNVVALAIGLEDFTADMGVQRTREGHE
                     SLMARNLLTLACKSAGVQAIDSVFSDVDDVEGLQNTIRVSKSLGFVGMGCIHPRQIRP
                     IHELYGPDEAEITKAKKIVLAFDDATAKGLGVVSLGSKMIDPPVVKRAIHTIDMAVKL
                     GKLAADWKEQMLTENA"
     gene            3192..4754
                     /gene="citF"
                     /locus_tag="PKY63_12115"
     CDS             3192..4754
                     /gene="citF"
                     /locus_tag="PKY63_12115"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPF01404.1"
                     /translation="MKKCDNFVVNAIGRKVPTIVNGKEVVPFKGLRQHRPTGRKYAPP
                     IPTCIDYPDDGNKVVSSLAEALKKCGLKDGMTISTHHHLRDGDLVSNTIFDIAHDMGV
                     KDLVWLPSASFPCNDPLIKYLEDGTINRIEGSMNGPLGKFTTKGGMTGCAVLRSHGGR
                     VQAIQDGEVHIDIAVVAAPVADPFGNANALSGKSACGILGYSLADYMYADKVIIVTDN
                     LIPFPCMPMQVQGNYVDYVVVVDQIGIPEKIVSGTTQVTRSPDRLLLAEWTAQFCDEA
                     GLLRDGCGMQSGAGGTSLAVGIYMEQILKSRGEKARFAFGGSTKYMVQMLENGSLDYI
                     LDAQAFDLEAVRSARENPNHIDLSVFQAYNYHSKGNYTSMTDIMILGATEIDMNFNGN
                     VVTHSDGLLLHGIGGWQNCLHAKTVIIPIPLFRDRIPVLVDEVTTICGPGEMIDVIVT
                     ERGIAINPKRTDLIEKMKNSKLPIKTIQELKAEAEAICGKPERPKFTDEAVAVIKWVD
                     GTVIDTVWKLAE"
BASE COUNT         1479 a         1209 c         1310 g         1415 t
ORIGIN      
        1 gcaggaagaa taaccgggct gctgaaagtt aacatgtatg cattgattgt actgtcaatg
       61 atggttgtgt cgcttgtcgc aacaaatgtt gaaggcatac cacccaacat ggaataaata
      121 acgccaaagg ttgtgtctcc attaacaggc gtttcgagtt gaatccagat ggcttcaatg
      181 gtgtcagtct gaaaaagttc aaacatggaa gccgcatatg cccaatcaga agaagaaaca
      241 ttatatggtg ttccggtaga gactccgttg tcgcgggcat acacagaatc tgtaacctca
      301 atgttgaatc tgatcgtatc gttcgatgca tttccgtcgg taaaagctgc atttcctgac
      361 agaatcaatt caatcatgta agtgcctttc ccattgggag gggtgaacgc ggatgaaact
      421 aaagtatcgg ttgcactgaa agtaagattt gtcacctgca cttgctcgct gctgagaagg
      481 ttgccgttga aataataatt aacatcgccg aaaacggtgt caggattatt cagtccttca
      541 gtcctgaaag ttgcttcaaa ggtttccgca aatacctgtg attccggaat ctgagtataa
      601 ccatgaattg gctttattga taaagcaata atatcatcga ctattgttgt gggatcaagt
      661 tcaattgcaa aagcaatatt gtcaggtgtt ccttgcagca tgcccataaa cgaaatttct
      721 ccgggtataa gttggtttgt aataaagcaa ccaccggcca gacctgaagt aataccatac
      781 aacgtttgaa gcggaggaaa catatcatag gtgaacgctg ttggcaagcc cgttgtcaga
      841 tttatggctg atacctgact taaattggct ccatcctgat gaaaaaccca gagaaaggga
      901 ccaccggtcg aataattgtc aacagcagca ccatacatac cgctgagcgt atgcgttgca
      961 gcaggaattg tagtaagcac agcccctgac atgctgatgg catcaatatc tgtattgaag
     1021 tttcctgtcc agaatccgcc ggctccactg ttgagagttg catcataggt cagaaaacgg
     1081 cttgtgctga ccgatgcact ggtaatgtgc ggaggtgcaa gagttgctgt aaccggatct
     1141 acacgataaa tcgtattggt attgttcccg atatacagat aggtgccatc agtagtgatg
     1201 ctgcgggttc ctgttagtcc ggcaataata aatttttcaa tcattgtgcc gtcaatatca
     1261 aagcgaatca gtgtgtcgct gccccatttg gatgcccata tttcactgtt aaccaatgcg
     1321 atggctgcca ttccggttga cccaccgata gagtctgagg cattgaatga catttgtata
     1381 tcccactgtg ctttgagagg acaggaggtt gattggtgat tcagaaacga attattgcta
     1441 gcctgaggat ccttttcact ttgtgccaaa gcaaagaaag atagaaaaac tgcaattgat
     1501 gtaatgaaag tttttttcat ggtattttgg atttgattgc aaaagtatta aaaaaatatt
     1561 aattcgattt ttcatggctt aataaatgat atttgtgttc gtataaggcg attttaatga
     1621 ctgttataga tatttatatt ctgtgcgagt gtttaggttg gtttaaattg tttaaatatt
     1681 tataaacgct gtcgcgtttt ttcaattacc gggactgaaa ctaaattgaa ttttgtgacg
     1741 cccctctgga atcgtcggaa agattgggaa tcccgtaatc aacgatccgg ggaccgcacc
     1801 attcaacaag aaaatcaccc gcgggagttt atcctgttgc ggcaggacca aaatgttgga
     1861 tactcttcga actatcgaaa gagttatttc tcaattatgg agttttgagc ggcacattcg
     1921 tattttttgt agttttgacc ccacaattca acactactca atatgaaaaa aaatgtggtt
     1981 gtctcttgcg ggaactcagg ccccaaagtc cgctctgact gccggattga aatgcagatt
     2041 accgaccagg ggggcattca gattgaactg aaaagcaagg tcaaagtgct gtatggcaac
     2101 gatattatcc gtgtgaccaa agaagtcgcc ggtttttatg gaattgaaaa ctgtcagatt
     2161 tccatcgagg attcgggcgc agtcactccg gtgctggcag cacgccttga agcggccatc
     2221 cggcagttgg ttgataccga caaggagtat ctcctgccgg tgatcccgca aaatcactat
     2281 gagactgaaa aagaacgcaa ccggatttcg cgtctgtatc tgccgggcaa ttcgccggcg
     2341 atgtttctga atgccggcat tcatcatccc gatgggctga tcctcgacct cgaagatgcg
     2401 gttgcgcccg ataaaaaatt cgaagcggca ttcatggtgc gcaatgcact gcgcaacatc
     2461 aatttctatg gtgctgaaaa aatggtgcgt atcaatcaga ttccgcgcgg tctcgacgat
     2521 ctgaaattca ttgtcaatca tggtgtgaat ctcattcttg ttccgaagtg cgagagtgca
     2581 gaacagattc acatggtgaa tgataaaatt gcagaaatcg ccaccgacaa aaaccgcaac
     2641 atctggctga tgccgattat cgaaagtgcc gatggcgttt tgcgtgcaaa agaaattgca
     2701 tctgctgctc ccaatgtggt ggctctggcc atcggactcg aagatttcac cgccgacatg
     2761 ggcgtgcagc gcacccgcga aggccacgaa tcgctcatgg cgcgcaacct gctcaccctg
     2821 gcctgtaaat cggccggcgt gcaggccatc gactcggtgt tcagcgatgt tgacgatgtt
     2881 gaaggattgc agaacaccat cagagtatcg aaatcgcttg gttttgtagg tatgggctgc
     2941 attcacccgc gccagatcag gccgattcac gaactgtatg gccccgatga agctgaaatc
     3001 accaaagcga aaaagatcgt gcttgcattc gacgatgcca ctgccaaagg tcttggcgta
     3061 gtttcgcttg gcagtaaaat gattgatccg ccggtggtaa aacgagccat tcacaccatc
     3121 gacatggcag tgaaactcgg caaactggct gcagactgga aagaacaaat gttaaccgaa
     3181 aacgcttagt catgaaaaag tgcgataatt tcgtagtaaa tgctatagga cgcaaagtcc
     3241 ccaccattgt aaacggtaag gaagtggtgc ctttcaaagg tctgcgtcag caccgtccaa
     3301 cgggacgcaa gtatgcgccg ccgattccga cctgtattga ttatcccgac gatggaaaca
     3361 aagtcgtttc atcactggcc gaagcattga aaaaatgcgg tctgaaagac ggcatgacca
     3421 tcagtaccca tcaccatctg cgcgatggag atctggtgag caataccatt ttcgacattg
     3481 cacacgacat gggtgtaaaa gaccttgtat ggctgccttc agcctcattt ccatgtaacg
     3541 atcctttgat caaatatctc gaagacggca ccatcaaccg cattgagggc tcgatgaacg
     3601 gcccgctcgg gaaattcaca accaaaggcg gcatgaccgg ttgcgctgtg cttcgttcgc
     3661 atggcggccg cgttcaggcc attcaggatg gcgaagtaca catcgatatt gctgttgttg
     3721 cagctccggt agccgatccg ttcggcaatg ccaatgccct cagcggcaag tctgcatgcg
     3781 gtattttggg atactcgctc gctgattaca tgtatgccga caaagtcatt attgtcaccg
     3841 acaacctgat tccgttccca tgtatgccta tgcaggtgca gggaaattat gttgactatg
     3901 ttgtggtggt tgaccagatc ggtattcccg aaaaaattgt ttccggaacc acgcaggtta
     3961 cccgcagccc ggatcgtttg ctgctggccg aatggacagc acagttctgc gacgaagccg
     4021 gtttgttgcg cgatggttgc ggcatgcagt cgggtgctgg cggaacatcg ctcgcagtgg
     4081 gcatttacat ggagcagata ttaaaatcgc gtggcgaaaa agcccgcttt gcgtttggcg
     4141 gaagtaccaa atacatggtg cagatgctcg aaaacggctc gctcgattat attctcgatg
     4201 cgcaggcatt cgatctcgaa gctgtgcgct cggcacgcga aaacccgaat cacattgacc
     4261 tttctgtatt tcaggcctac aattatcata gtaaaggtaa ttacacctcg atgaccgaca
     4321 ttatgattct cggtgcaact gaaattgata tgaatttcaa cggcaacgtg gttacgcact
     4381 ccgatggatt gctgctgcac ggtattggtg gctggcagaa ctgtctgcac gcgaagaccg
     4441 tcatcattcc cattccgctt ttccgcgacc gcattccggt gctggttgac gaagtgacca
     4501 ccatctgcgg ccccggcgaa atgatcgatg taatcgtgac tgagcgcggc attgccatca
     4561 atccgaaacg caccgacctc atcgaaaaga tgaagaacag caagcttccg atcaagacca
     4621 ttcaggaatt aaaagctgaa gctgaggcca tctgcggaaa accggagcgt ccgaaattca
     4681 ccgacgaagc tgtcgccgtt atcaaatggg tcgatgggac tgttattgat acggtctgga
     4741 agctggcgga atagaatatt gaacactgaa caaggaagtg tgaaggaaga agtaagtttg
     4801 aagtagatat ccttcgaaat tcttacttcc ttgcctgcct gttgcagaca ggttcgctgt
     4861 acagagttca tttgcgccgg gatatcctgg aaaacctttg cagccactca aatctcttcg
     4921 cagcaaatga catccggagg atgtcaaata ttaatagtgt ccgccggctg gcggatgatt
     4981 catagcaaaa caaaatccga ccctgatggg gtcgaatgat ttctatatgt tatacggatt
     5041 tgtttattgt gttcattttt gaacatttca taaaggatga atttcgggga ttatatgtcc
     5101 cgaatatatg gatatacggt gattatcatc cctaaaaaaa agggagcaaa aacgtgctcc
     5161 cttttcatta attcttctat tcttctggcg cggatttgta accgaagcgt tggaaacgct
     5221 gagctcatga acacctttaa aggttgtata atgagtgaat aatccgtgac agaaattatt
     5281 taatcaccga caacctcctc gtcagcatca catccttgta ttcgagtgta tatacataaa
     5341 tgccctgacc aagattgctg agatcgacat tcaccgaatt ctttccgctc tgaccatcaa
     5401 tagttgtagt ctg
//
LOCUS       DAOPKE010000143         5276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_16941_length_5276_cov_12.341314, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000143 DAOPKE010000000
VERSION     DAOPKE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16941_length_5276_cov_12.341314"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..85)
                     /locus_tag="PKY63_12120"
     tRNA            complement(3..85)
                     /locus_tag="PKY63_12120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:taa)
     gene            369..2477
                     /locus_tag="PKY63_12125"
     CDS             369..2477
                     /locus_tag="PKY63_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HPF01405.1"
                     /translation="MPFSKVFIRWFVCFFVFFFPLFLQAQTLEFDLRTKKVKQQDSLS
                     LQIRTYETIIDEMQRQPSSYESLASIMTQLGDLYSANEDYPKAIYLYMRSIIMRQMGL
                     ASGNQNKSSVAWQVIEVGNSFYRLHDYQLAENAYRIAAQYFSKTSDKLGLITSLNNIG
                     LCRLNLNKPGAALPLFRKTLAFSRQINDVPRIFSSSIYISISLKELHRFDDAIAVLNS
                     LTDYELDRDHLHLEDYRKLQMGETYILFGDTLKGIQIYEQLIEEAHDESAIYYKAITL
                     SRLGVFYDEMGDKQKAMEYTARSYELLKDRHHLTLLTSVNQLLYRLYKSSGDISKALF
                     HFEAYHEGIMQLNNREIENFVNEYNKKMERIAISQELKTMRVNSDRILLEKSNQQSLS
                     VFLVIIALLLLVMLYTAKGFDVRVQMLVDHVRSYSRQEKVYLLLLLGVYFVAFYYFFV
                     PVENAARLHNLSSFQRVLPGLIAYGVTFLLVLVFYSPFSFQKKVKNWYVYSLYFFSFA
                     FLSVLFFEFIHFNIRGFGGFNFLISLSLIVLASFIVPLYLFILIVEKLIIRHVESISE
                     LLNQHIGQMKLNPGPDKKMVTLQSEKTSGRLTFEISELMSVEAQGNYSMFVLLQNNLV
                     NRKILHITMKAIEESLAEYEFIVRCHKSYMINIQHIEKVTGNSRGYLVHFNVDADPVP
                     VSRNFQKNVMEVIRKFKDGF"
     gene            2626..5238
                     /locus_tag="PKY63_12130"
     CDS             2626..5238
                     /locus_tag="PKY63_12130"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01406.1"
                     /translation="MNVRVSKTKWTSIAIKIVTAMMLFCLLMAGVRSAQAAVSITAPT
                     LNISTASFPSAYFTLGDIVIDEGANGDFSFAAQNTNYSFTIHAPANFEFLATSGIISA
                     IAGDITSCSMNLSATQIVVTYSSDGSNVTQRSNDDDIITISGLQVRAITGPATQTATK
                     SASSEIINGLNNGTVLANLSSSLLSGCTHTITLTDTYGDGWNGGTVDVTVNGVTVLSG
                     ITLAAGAGPENHTFNADNGDVIVITRTDDGTYPAEMRVEITGGSANTLLAQQEPVAAG
                     TTVMGCCAPTVPNLANYVSPLNAAVNQSVCGVILDWDAPASNGCNAVSSYDVYFGTSA
                     MPPLVGNTTNTDYATGVLSSSTVYYWKIIPKNGVGSAIGASTYSFTTGATICAACMHT
                     IRLTDTYGDGWNGGTITVTVNGTPVLTNISLAGLSGPEDFTFNAASGDIINVTRTADG
                     SFPSEMQVEILSGGGTILLATTNPLVAPGTDITGCCPLVVPGCASVPVPVNGESLVNP
                     CSVDLAWTAPAASGCEGASSYDIYFGTTASPAFLINISGTSFVFPYALSDNTTYYWKI
                     VPKNSLGSATGCSTWSFSTSSSPNPAYCLYGSAINYPGGGTNCAQMTDELNAQNGCVW
                     NRGTISFASAFDYTINMYFGNNTGGADGCAFVFQNSPQGISTCGNNGSQLGAGGIPNS
                     LIIEFDTYDNDGTESNDLAVDHVAIEIDGDLPDDPANLPANAAPLCGPVQADPLDAYI
                     DDGLMHALRVTWDPATKDLCVYVDGSQRLCCNHDFINSVFGGNPNVYWGFTGATGGYT
                     NQQYFCPISIPLPVEMMDIKADCNYNKPILTWYTASETNNHFFTIERSEDGEKFDTIS
                     RVNGAGNTNEVSMQ"
BASE COUNT         1428 a         1016 c         1153 g         1679 t
ORIGIN      
        1 tgtaccccgg gcgggacttg aacccgcacg acccgaaagt cacaggattt taagtccggc
       61 gtgtctacca ttccaccacc ggggcctaaa agcgcctgca aaggtaataa gatttgtaat
      121 atttcctctt ttttattctc tttgtttaaa ctgttttggt ggatgtttgt tttgatttta
      181 aatgaatttc atggttcttt gataaccaga caatattttt tgacaaaagg gaatggttgt
      241 ttttgcgcat ggaaaaggcc ttacaaaatc attaacaaac acaatgatgt ctattcatag
      301 aattcttaat tctgtaagga tggtttttat atatctttgc gcaaacacca tttataaatt
      361 atttttttat gcctttttca aaggttttta tacgctggtt tgtctgtttt tttgttttct
      421 tttttcctct ttttcttcag gcacaaacgc tggagtttga tttaagaaca aagaaggtaa
      481 aacaacagga tagtttgtcg ttgcaaatca gaacgtatga gaccattatt gatgaaatgc
      541 agcgccagcc atctagctac gaatcgttgg ccagcattat gactcagctt ggagatctgt
      601 attcggccaa cgaggattat cccaaagcca tttatctcta catgcgatcc atcataatgc
      661 ggcagatggg acttgcatca ggcaatcaga ataaatcttc ggttgcgtgg caggtgattg
      721 aagtaggcaa ttcgttttat cgtcttcatg attatcagtt agccgaaaat gcgtatagaa
      781 tagctgccca gtatttcagc aaaacatcgg ataaactcgg tcttataaca tcgttgaata
      841 atattggttt atgccgtttg aatctgaata agcctggtgc cgccctgcct ttgttcagaa
      901 aaacgcttgc cttttcccgc cagattaatg acgttccccg tattttttct tccagtatct
      961 atatttccat ttctctgaaa gaacttcatc ggtttgatga tgccattgct gttttaaata
     1021 gtttaaccga ttatgagctt gacagggatc atctgcatct cgaagattat agaaaacttc
     1081 agatgggaga aacctatatt ttatttggcg ataccttaaa aggaatacaa atatatgagc
     1141 agctgattga agaggcacat gatgaatcgg ctatttatta caaagccatc acccttagcc
     1201 gtttgggtgt tttttatgat gaaatggggg acaaacaaaa agcgatggaa tatactgcca
     1261 gaagctatga attgcttaag gaccggcatc atctgacatt gttaaccagt gtcaatcagt
     1321 tgttgtatcg tttgtacaag tcttccggcg atatttcaaa agcgttgttt catttcgagg
     1381 cctatcacga aggaatcatg cagctgaaca accgcgagat tgagaatttt gtgaatgagt
     1441 acaacaaaaa aatggagcgg atcgccatta gtcaggaatt gaaaaccatg cgggtaaaca
     1501 gcgatcggat tcttctggag aagtcaaatc agcaaagttt atcggttttt ttggtcatta
     1561 ttgcgttatt acttcttgtt atgctttata cagccaaggg ttttgatgta cgtgtgcaga
     1621 tgcttgttga tcatgtacga tcctattcaa ggcaggaaaa agtgtattta ttgttgttgc
     1681 tgggtgtcta ttttgttgct ttttattatt tctttgttcc ggttgaaaac gctgccagat
     1741 tgcacaatct gagcagtttt cagagggttt tgcctggttt gattgcttat ggggttacat
     1801 ttttgctggt tttagtgttt tattcgccgt tttcttttca gaaaaaagtg aaaaactggt
     1861 atgtttattc tctctatttc ttttcttttg cttttttgag tgtgttgttt tttgaattta
     1921 ttcatttcaa tatcagaggg tttggaggat tcaactttct aatttcgctt tctcttattg
     1981 ttctggcttc gttcattgtt ccgttgtatc tttttattct tattgttgag aaactgatta
     2041 tcagacatgt tgaatccatc tctgagttat tgaatcagca tatcgggcaa atgaaactga
     2101 atccgggtcc ggataagaaa atggtaaccc tgcaaagcga aaaaacaagt ggccgcctta
     2161 cttttgaaat atctgaactt atgtcggttg aagcacaggg taattattca atgtttgttc
     2221 ttttgcaaaa taatcttgtt aaccgtaaaa tattgcatat aacgatgaag gctattgaag
     2281 aaagtttggc cgaatatgaa tttattgttc gttgtcataa gtcatatatg ataaacattc
     2341 agcatataga aaaagttacc ggaaactcac gtggatatct tgttcatttt aatgttgatg
     2401 cagatccggt tccggtttcc aggaattttc agaaaaatgt tatggaggtc attcgtaagt
     2461 ttaaagatgg cttttgaaaa gataaaatgt ccttcttgtc aaaaccataa cccatttgtc
     2521 attttttgat gtatcttgta aaatacaatc gaggggtatt aatcgattaa aatatatttg
     2581 caatcgacaa attaatgtcg aaccgtaaat ttaagcaccc ccactatgaa tgtacgtgta
     2641 tcgaaaacca aatggaccag cattgccatt aaaatagtta cagcaatgat gttgttctgc
     2701 ctgttgatgg ctggagtcag gagcgcacag gcagctgtga gtattaccgc tcccacactc
     2761 aatatttcaa cggcatcatt tccttctgcc tattttacgc tgggcgatat tgtgattgat
     2821 gaaggagcta atggtgattt tagttttgcg gcacaaaaca caaattattc tttcacaata
     2881 catgctcctg ccaattttga atttcttgct acttcgggta taatttcggc cattgctgga
     2941 gatataacgt catgttcaat gaatctttca gcaacacaaa ttgttgtaac atatagtagt
     3001 gatggatcaa atgtaactca gagatccaat gatgatgaca ttattacaat ttccggtctt
     3061 caggtgcggg caattacagg gccggcaact caaacggcaa caaaatcagc ttcttcggaa
     3121 ataattaacg gccttaacaa tggaacagtc ttggccaatt tatcatcgtc attattatcc
     3181 ggctgtaccc ataccattac cctaactgat acctatggtg atggctggaa cggtggcact
     3241 gtggatgtta ctgtgaacgg cgtaacagtt ttatcaggta ttacactggc tgccggcgct
     3301 ggtccggaaa accatacctt taatgccgac aatggcgatg tgattgtgat tacaagaacg
     3361 gatgacggga cttaccctgc agaaatgcgg gttgaaatta cgggcggatc agccaacacc
     3421 ttgcttgcac agcaggagcc ggtggcagca ggaacaactg ttatgggctg ctgtgcacca
     3481 actgttccca atctggccaa ttatgtcagt ccgctaaatg ctgctgtaaa ccagagtgta
     3541 tgtggggtta ttttggattg ggatgcgccg gccagtaatg gatgcaatgc tgtttcatcg
     3601 tacgatgtat atttcggaac ctcggctatg ccgcccctgg ttggaaacac aaccaatacc
     3661 gattatgcca caggagtatt aagttcttcc acagtatatt actggaaaat tattccgaaa
     3721 aatggcgttg gaagcgcgat tggtgcatca acatattctt ttacaaccgg agcgacaata
     3781 tgtgcggcct gtatgcatac catccgattg accgacactt acggagacgg ctggaatggc
     3841 ggaactatta ctgttactgt taacggaacc ccggttttga caaatatttc gctggcagga
     3901 ttgagcggtc ccgaagactt tacttttaat gctgcttcag gcgacataat taatgttacc
     3961 cgaacggcag acggttcttt tccttccgaa atgcaggttg aaatattgag tggaggcgga
     4021 acaatcttac tggccacaac caatcctttg gttgctcccg gcactgatat tacgggttgt
     4081 tgtcctttgg tagttccagg ttgtgcttcg gttccggttc cggttaatgg cgagtccctt
     4141 gtgaatccct gttctgttga tctggcctgg acagcgccag cggcttcggg ttgtgaaggt
     4201 gcatcttctt acgatatata ttttggaact actgccagtc cggccttttt aataaatatc
     4261 agtggaacct cttttgtatt tccttatgct ctttccgata ataccaccta ttattggaaa
     4321 attgttccga agaacagtct tggcagtgcc accggttgtt caacctggag tttttccacc
     4381 agcagttcgc caaaccccgc atattgcctc tatggcagcg ccatcaatta tccgggagga
     4441 ggaacaaact gtgcgcaaat gactgacgaa ctcaatgctc agaatggatg tgtctggaat
     4501 cgaggaacca tttcttttgc atcagctttt gactatacaa ttaatatgta ttttggaaat
     4561 aataccggtg gtgctgatgg ttgtgccttt gtatttcaga attcgcccca gggtatttcg
     4621 acctgtggaa acaatggatc tcagttgggt gcaggtggaa ttcccaattc cctgattatt
     4681 gaatttgata cttatgataa tgacggaacg gaaagcaatg atcttgcggt tgaccatgtg
     4741 gccattgaaa ttgatggaga tttgcccgat gatcctgcaa atctgcccgc gaatgccgcc
     4801 ccattatgcg gtcctgtaca ggcagatccg ctggatgcat atattgatga tggtcttatg
     4861 catgctttgc gtgtcacctg ggatccggcc accaaggatt tgtgtgtata tgttgacgga
     4921 agtcagcgac tgtgctgcaa tcacgatttc atcaactcgg tgtttggcgg aaaccctaat
     4981 gtttactggg gatttacggg tgctaccggt ggttacacca atcagcagta tttctgcccc
     5041 atcagtatcc ctctgccggt tgaaatgatg gatattaaag cagattgcaa ttataataaa
     5101 ccaatactta cgtggtacac tgccagtgag acaaacaatc attttttcac cattgaacgg
     5161 tcagaagacg gagaaaaatt cgataccatt tcacgggtaa atggagcggg aaatacgaat
     5221 gaagtatcca tgcaataaaa atggtctttg agtaagttgt taagagtaga ctaact
//
LOCUS       DAOPKE010000144         5246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_17088_length_5246_cov_10.716818, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000144 DAOPKE010000000
VERSION     DAOPKE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17088_length_5246_cov_10.716818"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKY63_12135"
     CDS             complement(<1..890)
                     /locus_tag="PKY63_12135"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPF01407.1"
                     /translation="MKAFINRLAVILILTVSGFNSFGQLTVTSGLTASQYVDFLVGSG
                     ITYSNAVYTGDPAAIGKFVTGATPTNLGLSSGVIMSSGYVNGTGTEAIGSAAINFVST
                     PFTNTYTDANLQSLIPSYTVEDATKIEFDFIPVSDTIKFRYVFGSEEYLEWVSSSFND
                     VFGFFISGPNPAGGAYSSNNIARIPGTALPVTIDNINTGSYSQYYVDNEGLGGTTIVF
                     DGFTTVLTAWALVTPCVQYHIKLAVGDAGDQSYDSGVFLEANSFSSPTLNVSTTYVNE
                     TTIGSFAMEAGCNDVTTCFS"
     gene            complement(896..2548)
                     /locus_tag="PKY63_12140"
     CDS             complement(896..2548)
                     /locus_tag="PKY63_12140"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPF01408.1"
                     /translation="MFHRPIIAFFCFLLAFFGLRAQISVDYSLTPTQLVQNVLLGGGV
                     SVSNVIFTGGTEMRGTFEGLSNLGIDTGLILATGDIAISIGPNDESSASDGGNVSGDS
                     QLDALVGSTTYDAAVLEFDFVPSSDTIRFFYVFGSEEYPEYVCSDFNDVFAFFLSGPN
                     PLGGNYVNENIAKIPGTTIPVAINSINPGVTGAYGTAGGCTSLAYSSLYTDNTSGTTI
                     QYDGFTHVLEASANVVACSNYHIKIAIADVTDGAYDSGVFLKAKSFSSPAVNISAVGS
                     SFDSTMVEGCGYATYTFTRGGDLSNPFTINYLIEGDAINGIDYTDLAGNPIPSSVTFA
                     AGQNSVDLNIDPVNDALSEFTEQIKLSVPQVISCVDDTLSATIYIINVDPMTIELTGD
                     TLICVEEGEFTTLNVDFTGGYGPFDFAWDNGLGNSNQVIVAPEHPTTYTVSILDSCGN
                     ESAVKSITIHNECEIRLTNVVTPNSDGFNDFVHFANLEMYPNSELLIFNRWGKIVYKN
                     DCYNNDWSPTDFSEGTYYYVLKKTAGIEHPAKEYHGTLTILK"
     gene            2737..4098
                     /locus_tag="PKY63_12145"
     CDS             2737..4098
                     /locus_tag="PKY63_12145"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPF01409.1"
                     /translation="MNEIRNYIENNKERFLNELFDLIRIPSISSESAHKNDMVRAAEY
                     WKKAILDAGADFAEVCPTTGNPVVYGEKIIDPSYQTVIVYAHYDVMPVDPLSEWKTDP
                     FEPVIIDEKIYARGADDDKGQGFMHAKAFEYLMKNNLLKCNVRFMIEGEEEIGSPAMV
                     EWCKNNKDRLKGDIILVSDTGMIAADIPSITVGLRGLTYLQIELTGPNRDLHSGLFGG
                     AVANPINMLSKMIASLTDEKGRITIPGFYDDVDVVSEAERTALNKAPFSIDNYKTAID
                     VAELFGEEGYTTIERTGIRPTLDMCGIWGGYTGEGSKTVLPSKAYAKLSSRIVPHQDH
                     HKVAELIKAHLEKIAPPSTKVKVEVLHGGQPYVMPIEGKAYQAADAALTEIFGKKPVP
                     TRSGGSIPIIATFEEILGVKSLLMGFGLESDAIHSPNENYRVENFLNGIEAIVRFYLN
                     FQK"
     gene            4182..4832
                     /gene="upp"
                     /locus_tag="PKY63_12150"
     CDS             4182..4832
                     /gene="upp"
                     /locus_tag="PKY63_12150"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPF01410.1"
                     /translation="MVHHLGKENSIVNQFISELRDAEIQQDSLRFRKNLERLGEIFAY
                     EISKKIPYIPRPVTTQLGEVDVPVVAKWPVLATILRAGLPFHQGFLNIFDQSESAFIS
                     AFRKHHKDGRFTIQVEYVSCPDLDGKTLVIADPMLATGSSLVLSAKELFQFGMPEEIH
                     VATVIASTQGIDYVRQHLPHRKLHIWAAAVDEELTAHAYIVPGLGDAGDLAYGRKD"
     gene            4842..>5246
                     /locus_tag="PKY63_12155"
     CDS             4842..>5246
                     /locus_tag="PKY63_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01411.1"
                     /translation="MPCLLLLTHRFYEYAYVVVISTYRFLFAGPVAVASGFDYLEISL
                     SLALGGFIGFVVFYFFSDRVIYLYRMLLQKNQKPPKSGWMKRYRKQLQLRRYGIPLLM
                     VFGPSVLSIPLTAFIVRRWFRKTKCIIVYFCIS"
BASE COUNT         1473 a         1011 c         1231 g         1531 t
ORIGIN      
        1 aatgaaaaac aggtcgtcac atcattgcag ccggcttcca tggcgaagct tccgatggtt
       61 gtttcgttca cataggttgt cgaaacattc agcgtaggac ttgaaaagct gttggcttcg
      121 agaaaaacac cggagtcgta agactgatcg ccggcatcgc cgacagccag tttgatatgg
      181 tattgaacac agggagtaac gagtgcccat gcagtaagca cagttgtgaa tccatcaaat
      241 acgatggtgg tgccgcctaa accttcgtta tctacataat actgactgta ggaacccgtg
      301 tttatattgt cgatggtaac aggcagtgct gttccgggta tacgggcaat attattactt
      361 gaataggcgc ctcctgcagg attgggacct gaaataaaaa atccgaaaac atcgttgaag
      421 ctgctgctga cccattcgag atattcctcc gatccaaata catagcggaa tttgatggtg
      481 tcggaaaccg gaataaaatc gaattctatt ttggtggcat cttcaacagt gtaggacgga
      541 ataagtgatt gcaggttggc atctgtatac gtatttgtaa acggtgtgct tacaaaattg
      601 atagccgcac taccaattgc ctctgttcct gtgccgttca catagccgga tgacatgatt
      661 accccggagg aaagacctaa attggttggt gttgctccgg tcacaaactt accgattgct
      721 gctgggtcgc ccgtgtatac agcattgctg taggtgattc cagatccaac cagaaaatcg
      781 acatactgcg aagctgtaag cccggacgtg acggtaagtt gtccgaaact gttaaaaccc
      841 gaaactgtta aaatcagaat tacagccagc cggttaatga aagctttcat ttcgtttatt
      901 ttaggatggt cagagtgcca tgatattctt ttgcaggatg ttcgatacct gccgtttttt
      961 tcagaacata gtagtatgta ccttcagaaa agtcagttgg agaccagtcg ttgttgtagc
     1021 agtcgttttt ataaacgatt ttgccccaac ggttgaaaat cagcaattcg gaattcggat
     1081 acatttcgag attggcaaag tgtacaaaat cgttgaaccc atctgaattg ggtgtaacta
     1141 catttgtgag gcggatttcg cattcgttgt ggatggtgat tgattttacg gctgattcgt
     1201 tgccgcatga atcgagaatg gaaacagtat atgttgtggg atgttccgga gcaacaataa
     1261 cctgattgct gttgccaagt ccgttgtccc aggcaaaatc gaagggtcca taaccaccgg
     1321 tgaaatcaac gttcagggtg gtgaattcgc cttcttcaac gcaaatcagt gtgtcgccgg
     1381 tcagctcaat ggtcattggg tcaacattaa tgatgtaaat ggtggcagaa agagtgtcat
     1441 ctacacatga gattacctga gggacggaaa gtttaatttg ttcggtaaat tctgatagcg
     1501 cgtcgttaac agggtcaata ttcagatcaa cgctgttctg gcctgcagca aatgtaaccg
     1561 agctgggaat gggatttccg gcaaggtcgg tataatcaat cccgttgatg gcatcgcctt
     1621 caatcagata attgatggtg aatgggtttg acaggtcacc acctcttgtg aaagtataag
     1681 ttgcgtaacc acaaccttcc accatggttg agtcgaaaga actcccgacg gcagagatat
     1741 ttacggcggg agaactgaaa ctctttgctt taagaaaaac cccggaatca tatgccccat
     1801 cggttacatc ggctatggca attttgatat gatagttgga acaggcaacg acgttggccg
     1861 aggcctccaa aacgtgcgta aatccatcgt attgaatggt tgtacctgat gtgttatcgg
     1921 tataaagcga agaatatgcg agcgaagtgc atccgcctgc tgtgccataa gccccggtta
     1981 cccccggatt gatggagttg atggcgacag gaatggttgt tccgggaatt tttgcaatat
     2041 tttcatttac ataattgccg cccagtgggt ttggtccgga cagaaaaaat gcaaaaacat
     2101 cgttgaagtc agaacaaaca tattcggggt attcttcact tccgaaaaca tagaaaaacc
     2161 ggatggtgtc cgatgatggc acaaaatcga attcaagaac tgctgcatca taggtggtgc
     2221 ttccaaccaa tgcgtctagt tgggaatcgc ctgaaacatt gccgccatcg ctggcgcttg
     2281 attcgtcgtt ggggccaatg gaaatggcaa tatctcctgt ggccagaatc aatccggtgt
     2341 caatccccag attggataat ccttcgaatg tcccacgcat ttctgttcca ccagtaaaaa
     2401 ttacattgga aaccgaaaca cctcctccaa gtaaaacatt ctgtaccagt tgagtagggg
     2461 ttaaagaata atcaacggaa atctgagccc ttagtccgaa aaaagcaagt aaaaaacaga
     2521 aaaaagcaat gattggtcta tgaaacatgg cggaaataat tgtaattttt acaaagatat
     2581 tatttatatc taaatgattg catctaaaat gcttttttta tttgattcgc gtaccatttt
     2641 gtcaaaaaat ctaattattg taatcaagta tcagaatgct tttatcggtt catttcaatc
     2701 atataaattg ttatctttgt ttcaaaatcc aaaacaatga acgaaatacg caattacatt
     2761 gagaacaata aggaacgctt tctgaatgaa ctgtttgacc ttatacgtat tccctccatt
     2821 tctagtgaat cggcacataa aaacgatatg gtgcgggctg ctgaatattg gaaaaaggcc
     2881 attttagatg ccggagccga ttttgccgaa gtatgtccca caacaggcaa tccggtggtt
     2941 tatggagaaa aaatcattga cccatcttat caaacagtta tcgtttatgc acattatgac
     3001 gtaatgccgg ttgatccgct ttcggaatgg aaaaccgacc cttttgagcc ggtgattatt
     3061 gatgaaaaaa tttatgcgcg tggggctgat gatgacaagg gtcagggttt tatgcatgcc
     3121 aaggcatttg aatatctgat gaaaaacaat ctgctcaaat gcaatgtgag atttatgatt
     3181 gagggtgaag aggaaattgg cagtccggct atggttgaat ggtgtaaaaa taataaagac
     3241 cggcttaagg gtgatatcat ccttgtttca gatacaggaa tgattgctgc tgatattccg
     3301 agtattacgg tcggactcag aggtcttact tatcttcaga ttgagcttac cggtccgaac
     3361 cgggatcttc attccggcct ttttggtggt gctgtggcca atcccatcaa tatgctttcg
     3421 aaaatgatag cttctttgac cgatgaaaag ggccgaataa ccattccggg tttttatgat
     3481 gatgttgatg ttgtttctga agctgagcgt accgcgttga ataaagcccc gttcagtatt
     3541 gataattaca agactgccat tgatgtagca gaattgtttg gcgaggaggg ttacacaacc
     3601 attgagagaa ctggtattcg cccgactcta gatatgtgtg gcatttgggg cggatatacg
     3661 ggtgaaggat cgaaaacggt cttgccttca aaagcatatg caaaactttc ttcacgcatt
     3721 gtaccccatc aggatcatca taaagttgct gagttgatta aggcacatct tgaaaaaatt
     3781 gccccgccgt caaccaaagt gaaagtggag gtattgcatg gtggacagcc ctatgttatg
     3841 ccgattgaag gtaaagctta tcaggcggcc gatgcagccc tgacggaaat attcgggaaa
     3901 aaacctgtgc caacccgcag tggaggcagt attccaatta ttgcaacctt tgaggaaatt
     3961 cttggtgtaa aatcactttt gatgggattt ggacttgaat ccgatgctat tcattcgcca
     4021 aatgaaaatt acagggtcga aaattttctg aatggtattg aggccatcgt tcgtttttac
     4081 ctgaattttc agaagtaaaa cagatgattt ttgccggatt cttttttgtg taaaagaaaa
     4141 gtaaaaaata agtattttag cccctttaac aaatttgtca tatggtacat catctgggta
     4201 aggaaaattc aattgtgaac caattcatca gtgagttgcg cgatgctgaa attcagcagg
     4261 acagtttgcg tttcagaaaa aacctggagc gactcggtga aatttttgca tatgaaataa
     4321 gcaaaaaaat cccgtatata cctcgtcctg ttacaacgca gctgggtgaa gttgatgtgc
     4381 ctgttgtggc aaaatggcca gtgttggcca ccattctgcg tgccggtttg ccgtttcatc
     4441 agggttttct gaatattttt gatcagtccg aaagtgcttt tatttctgct tttcgcaaac
     4501 atcacaagga tggtcgcttc accattcagg ttgaatatgt cagttgtcct gatctggatg
     4561 ggaaaactct ggtaattgcc gatccgatgc tggctacagg ttcttcgctt gttttatccg
     4621 ccaaggagtt gtttcagttt ggaatgccgg aagaaataca tgttgcaaca gtaattgcca
     4681 gcactcaggg aattgattat gtacgtcagc atttgccgca tcgcaaatta catatctggg
     4741 ctgctgctgt agatgaggag cttacagctc atgcctatat tgttcctggt ttgggtgatg
     4801 caggcgatct tgcctatgga cgtaaggatt aaactgataa tatgccttgt ttattgcttt
     4861 tgacacacag gttttatgaa tatgcatatg ttgttgtcat cagcacttac aggtttttgt
     4921 ttgcagggcc ggttgcagta gccagtgggt ttgattatct ggaaatatca ctcagtcttg
     4981 cgttgggtgg ttttattggt tttgtggttt tttatttttt ctctgacagg gttatttatc
     5041 tttacagaat gttgttgcag aaaaatcaga aaccacccaa aagcggatgg atgaaaagat
     5101 atcggaaaca gctgcaattg cgcaggtatg gcattccctt actgatggtt ttcgggcctt
     5161 cagtgttatc aattcctctt actgcattta ttgtccgtcg ttggttcaga aaaacaaaat
     5221 gtataattgt ttacttttgt atatct
//
LOCUS       DAOPKE010000145         4952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_18457_length_4952_cov_10.207270, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000145 DAOPKE010000000
VERSION     DAOPKE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18457_length_4952_cov_10.207270"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKY63_12160"
     CDS             <1..309
                     /locus_tag="PKY63_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPF01412.1"
                     /translation="QSMPIPADKTDIAVATALAGKFLGHKLIYLEAGSGALNAVPADI
                     VKAVSSKTNLPVIVGGGIRDEKTALDLFSAGANSIVIGSLFETNPDAVLRIAQQIRNH
                     "
     gene            328..2460
                     /locus_tag="PKY63_12165"
     CDS             328..2460
                     /locus_tag="PKY63_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPF01413.1"
                     /translation="MKRIFLFLLLPLLAFNVAVSQKQALTLDQIFTDRSLYPGYVSGL
                     QPHFSQNWFSYISDDDELKKVDAKGKEVVLLTLAELNTALSSAGAAEVKYFPEYSWVN
                     DSKIRIIEDNKWVEVDVTSKKAEVKNSWNKEAENIDLSPEKYVAAYTIENNLFISVAG
                     AEKAITSEKDKGVVCGQTVHRNEFGISKGIFWSPKGNKMAFYRMDETMVTDYPLVNIN
                     SRIAEVENIKYPMAGMTSHQVTLGVYDVVSGKTIFVKTGEPKEQYLTNISWSPDEKYV
                     FIGVLNRDQNHLQWNKYDAVTGEFIKTMFEEKSDRYVEPQTPLVFLPGSDDKFIWQSQ
                     RDGYNHLYLYNTEGQLLKQLTKGPWMVTEFGGFDTKAQKIWFTATKESPLENHAYIVN
                     IKDGKITKVTSEAGAHSIWLTRDFSLMCDYYSATDMGASQKIKTTAGKEIKSLLKNEN
                     PLKDYAVPTPEFITLKAEDGTPLYARLLKPVGFDPAKKYPVIVYVYGGPHAQLVSNSW
                     TGGAGMFLYYLASKGYVVFTLDNRGSANRGLNFESAIFRNCGTVEVADQMVGVEWLRS
                     QPWVDQTRMGVHGWSYGGFLTISMMLKQPGVFKAAVAGGPVIDWKYYEVMYGERYMDT
                     PESNPQGYKNAALTNYADKLDGRLLIIQGYQDNTVVPQHCLSFLDASIKAGKLVDFFM
                     YPNHEHNVRGKDRLHLYKMIDLYFEDHL"
     gene            2679..3950
                     /locus_tag="PKY63_12170"
     CDS             2679..3950
                     /locus_tag="PKY63_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01414.1"
                     /translation="MIKNIILQQQEEKHFLMNQPYIERIDDAEKSKLLKTKLIKVITG
                     PRRAGKSVMALQLLRKTNFAYLNFDDDLLLRHFTENTVLQALHQIYPGFDYLLLDEIQ
                     NLNDWELWVNKLYRRGVNLVITGSNSRLLSNELASGLTGRFVQLPVLPFGFAEIIRVS
                     EMLPAVRPQATLSESGKLMVILEKYLFNGGFPEVIMNQDILKNYLSSLFDSILLKDIL
                     KRFRIRQTQQLYDLSHYMLSNYTNCFTYSQVRESLGFNSVATVQKFTGYLEEPYLFQH
                     LTRYNTKVRHQQKAARKIYIIDNGFIKARSFELSPNYGRLLENLVFIELLRRNYLPEL
                     TLFYYRTRNDREVDFVLRNGNKIEQLIQVCYSMDQPKTIKRELNALVEASQELKCSKL
                     LVITWDRQDVIEQKNLSVDLVPAYKWFTEDV"
     gene            complement(4158..>4952)
                     /locus_tag="PKY63_12175"
     CDS             complement(4158..>4952)
                     /locus_tag="PKY63_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01415.1"
                     /translation="SKYIDYYENGNTSKIWEKTTFNNNPCEITRTYDGNGNIKNLIYQ
                     DQSDTLTYSSEQDFEKTQIRNQLQSAYNDFTQTVLKELKSSLEMKGSIDFNRNVKGEI
                     SFNLSIKPLDKSIDPSITDKFSSCISKTITNKSVILPVYNNAYSFQEQISIQVVDGKE
                     KVFVKSGKSLLSYGEIETESNNPKKVNTVCKNEIARKKIITDYSSKKAGVYKINYRTI
                     TINDIPSQIYLTEEKYKIHTVGGNILRYTGYTIALGVSLLLALAAQ"
BASE COUNT         1478 a          935 c         1095 g         1444 t
ORIGIN      
        1 caatccatgc cgattccggc cgataaaacc gatattgccg tggcgactgc gctggccggg
       61 aaatttcttg gacataaact gatttatctc gaagccggct cgggcgcact gaatgcggtt
      121 cctgctgata tcgtcaaagc agtttcatca aaaacgaact tacctgttat cgttggcggc
      181 ggaatcagag atgaaaaaac agctttggat ctattcagtg ccggagcgaa ctccattgtc
      241 atcggaagtc ttttcgaaac caatcctgat gcggttttac gcattgccca acaaatcaga
      301 aatcactaac aaacgttata gttttctatg aaaagaatat ttctttttct actgttgcca
      361 ttgctggctt ttaatgtcgc tgtttctcaa aaacaggcat tgacacttga tcagattttc
      421 actgaccgca gtttgtatcc cggctatgtg tcgggtctgc agccacattt ttcgcagaac
      481 tggttttcgt acatcagcga tgacgatgaa ttgaaaaaag tggatgctaa aggaaaagaa
      541 gtggtgttgc tgacgttggc agaattgaat accgcattgt catcggcagg agctgctgaa
      601 gtgaaatatt ttcctgaata ttcgtgggta aacgattcga aaatccgcat tattgaggac
      661 aataaatggg tggaagtgga tgtaacttcg aaaaaagcag aagtgaaaaa ttcatggaac
      721 aaagaagcgg aaaatattga cctgagcccg gagaaatacg tggctgcata taccattgaa
      781 aacaatttgt ttatttcggt tgcgggcgct gaaaaggcca tcaccagcga gaaagacaaa
      841 ggtgttgttt gcggacagac ggttcaccgc aatgaattcg gaatatcgaa aggaattttc
      901 tggtcaccga aaggaaacaa aatggcgttt taccgtatgg acgaaaccat ggtgaccgat
      961 tatccgctgg tgaacatcaa ttcacggatt gctgaagttg agaatattaa atatcccatg
     1021 gccggaatga ccagtcacca ggtgacgctt ggtgtttatg atgttgtaag tggtaaaacc
     1081 atctttgtaa aaaccggaga accgaaggaa cagtatctca ccaacatcag ctggagccct
     1141 gatgaaaagt atgttttcat tggtgtgctc aaccgtgatc agaatcacct gcagtggaac
     1201 aaatacgatg ctgtcaccgg tgaattcatc aaaacgatgt ttgaagaaaa aagcgaccgc
     1261 tatgttgaac ctcagactcc gcttgttttt ttgccgggat cggatgataa attcatctgg
     1321 cagagtcagc gcgacggata caatcatttg tatctgtaca ataccgaagg acagctactg
     1381 aagcagctta ccaaaggtcc gtggatggtc actgaatttg gtggctttga tacaaaagcg
     1441 cagaaaatct ggttcacggc aaccaaagaa tcaccgcttg aaaatcatgc ttacattgtg
     1501 aacataaagg atgggaaaat cacgaaagtc acatctgaag ccggagcaca cagtatctgg
     1561 ctgacaaggg atttttcgtt aatgtgcgat tattacagcg caacggacat gggcgcttcg
     1621 cagaaaatta aaacaacagc agggaaagaa atcaaatcgt tgctgaagaa cgaaaaccct
     1681 cttaaagact acgctgtacc gacacctgaa ttcattacac taaaagccga agacggaaca
     1741 ccgctctacg cgcggctttt gaagcctgtt ggttttgatc cggcgaaaaa atatccggtc
     1801 attgtgtatg tatacggagg tccgcacgca cagctggttt ccaacagctg gaccggtgga
     1861 gcaggcatgt ttttgtatta ccttgcatcg aagggatatg ttgtgttcac gctcgacaac
     1921 cgtggttcag ccaatcgcgg attgaatttc gaaagcgcca ttttccgcaa ttgcggcacg
     1981 gtagaagtgg ccgatcagat ggtaggtgtt gagtggctca ggagtcagcc ctgggtcgat
     2041 caaacccgca tgggcgttca tggctggagc tatggcggat tcctgaccat cagcatgatg
     2101 ctgaaacaac cgggcgtttt caaagctgcg gtggcgggcg gtccggttat cgactggaaa
     2161 tattacgaag tgatgtacgg cgaacgctat atggatacac ccgaatcaaa cccgcaaggg
     2221 tacaagaatg cagcgttgac aaactatgcc gataaacttg acggccgcct gctgatcatt
     2281 cagggatatc aggataatac ggtggttccg cagcattgtc tttcattctt agatgcatcc
     2341 atcaaagccg gcaagctggt cgatttcttc atgtatccca atcacgagca caatgtgcgc
     2401 ggcaaagacc ggctgcattt gtataagatg attgatttgt attttgagga tcatttgtag
     2461 aaatacgaaa tcagaaatac gagatacgaa atgcgaaagg atgtggcgcc ttcgtcggta
     2521 taactgacta tcttttgact ccactttttc gcagaaaact gatgatttta tcagacagca
     2581 cttttcgatg acatcagaca gtatggattt gcttttatgt tcaaaaaaat tgctgtaatg
     2641 aataaaaaag actatatttg ttcattaaaa ctcattttat gatcaaaaat atcattcttc
     2701 agcagcagga agagaaacat tttttgatga atcagccata tatcgagcgt attgatgatg
     2761 ctgagaagtc aaaattgtta aagacaaagc ttatcaaagt aatcacggga cccaggaggg
     2821 caggaaagtc ggtaatggct ctgcaacttt tacgaaagac aaattttgct tacctgaatt
     2881 ttgatgatga tttattgctg agacacttta cagaaaacac cgtgttgcag gcattgcatc
     2941 agatatatcc gggatttgac tatttattgc tcgatgaaat acaaaatctc aatgactggg
     3001 aattatgggt taataaattg tatcgtcgtg gcgttaattt ggtcataacg gggtcaaatt
     3061 ccagattgct ttcaaatgag ctggcatcag gtctgacagg ccgttttgtg cagttgcccg
     3121 ttttgccgtt tggatttgcg gaaatcatcc gtgtcagtga aatgttgccc gcagtcaggc
     3181 ctcaggcaac tctgtcagag agtggaaaat tgatggtaat actagaaaaa taccttttta
     3241 atgggggttt tcccgaagta ataatgaatc aggatatatt gaaaaattat ctctcatctt
     3301 tatttgattc catattgctc aaggatattc tgaaacgctt taggattcgc caaacacagc
     3361 aattgtatga tttgtcgcat tatatgttat cgaactacac aaattgtttc acttatagtc
     3421 aggttaggga atcgcttgga ttcaacagtg ttgcaaccgt tcaaaaattt accggttatc
     3481 ttgaagagcc ctatcttttt cagcatctca cccgatacaa taccaaagtc agacatcagc
     3541 agaaagctgc acgaaaaata tatataatag ataatggatt tataaaagcg aggtcatttg
     3601 aattgtcgcc caattatggc cgattgcttg aaaatcttgt ttttattgaa ttattgcgtc
     3661 ggaattattt gcctgaatta actttgtttt attatcgaac ccggaatgac cgggaagttg
     3721 attttgtgct tcgtaacggc aataagatag agcaactgat tcaggtttgt tattcgatgg
     3781 atcagccaaa aacaatcaag cgagagctga atgctcttgt cgaagcatct caggaattaa
     3841 aatgcagcaa attgttggta attacctggg atcggcagga tgtgatagaa caaaaaaacc
     3901 tgagtgtaga tttagtgccg gcatataagt ggttcacaga agatgtgtga gtcaccccag
     3961 acaaaagatg tgctgctcaa tcctcccctt cgtcgggatg aactccgcaa acggggcatg
     4021 ggctcttcat gtatcccaat cacgaacata atgtgcgcgg caaagaccgg ctgcatttgt
     4081 ataaaatgat tgatttgtat tttgaggatc atttgtagaa aaataaaggc cggaacaaat
     4141 tgaccggcct ttatttttta ttgtgcagca agggcaagca acaatgaaac cccaagagct
     4201 attgtatagc cagtataacg aagaatattt ccgccaacag tatgaatttt gtatttttcc
     4261 tcggtcaaat atatttgaga aggaatgtcg ttgattgtga tggtgcgata atttattttg
     4321 taaacgccag cttttttaga agaataatca gtaataattt tttttctggc gatttcattt
     4381 ttacaaaccg tgtttacttt ttttggatta tttgattcag tttcgatttc accataggat
     4441 aaaagagact tacctgattt tacaaacact ttctctttac cgtcaacaac ctgaattgaa
     4501 atttgctcct gaaaggaata cgcgttattg taaacaggta aaataaccga tttattggta
     4561 atggttttac tgatgcatga tgaaaactta tctgtaattg aaggatcaat acttttatct
     4621 aacggtttta tagaaagatt aaaactgatt tctcctttca catttctgtt aaaatctata
     4681 gaccccttca tttccagtga agatttaagc tcctttaata ctgtttgtgt gaaatcatta
     4741 taagcacttt gcagttgatt tctgatttga gttttttcaa aatcctgttc actgctatat
     4801 gtcaatgtgt ctgactgatc ctgataaatg agatttttaa tatttccgtt tccgtcatac
     4861 gttcttgtta tttcacacgg gttattattg aatgtggttt tttcccaaat ttttgaagtg
     4921 tttccgtttt cataataatc aatatattta ct
//
LOCUS       DAOPKE010000146         4791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_19276_length_4791_cov_13.218539, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000146 DAOPKE010000000
VERSION     DAOPKE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19276_length_4791_cov_13.218539"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKY63_12180"
     CDS             <1..234
                     /locus_tag="PKY63_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01416.1"
                     /translation="PFSSFLIPGIVLFVVNGVLNISAGILTIRNHAAAARFVMLQGLL
                     LCGWIVVQILMVRDINALHIVMFSIGVGLIGLG"
     gene            268..678
                     /locus_tag="PKY63_12185"
     CDS             268..678
                     /locus_tag="PKY63_12185"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HPF01417.1"
                     /translation="MENKELRWKQRYQNFEMAFSKLQEAMQQNELNELERNGLIQRFE
                     FTIDLSWKVLKDYLEEKGFVFKPSPKDTIREAQRAGLIDFGQALIDGIDIRNDLSHDY
                     SGNKFEQSETILRDTVFPALNQLNQFFILEDATK"
     gene            691..1032
                     /locus_tag="PKY63_12190"
     CDS             691..1032
                     /locus_tag="PKY63_12190"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPF01418.1"
                     /translation="MENTAELFGLSPRDIQTIHDVFSKYTDIETVRIFGSRATGSFKP
                     GSDIDLAIMNSVPSRIMLNLINDFEESSLPFKIDLIDFSSLTNPALTEHIQKAGIVFY
                     NRPSSKTENPG"
     gene            1110..1934
                     /locus_tag="PKY63_12195"
     CDS             1110..1934
                     /locus_tag="PKY63_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPF01419.1"
                     /translation="MITYRVFIRTLVLLFLLISGLNAGKISAQNTSAVTIPESRFYPA
                     LHRYSSIPADSSVVVHSFETTPFITLGEYKEFMKSVSGSEELPDSLMPDTSICIPMKY
                     KKYLSGKKYEKYPALGISWKNAIEYCIWRTKKENPDSGIVFIYRLPYKTEWIQMAQYA
                     DSALKKFEINRHYAEWIMASFDESALSFPGNLNNAYTFEYLYLAGKSDPQVLKRMCIY
                     GKSFLSNHQKNLYGYSDQGYRDVGFRMVKEYVNVPGRKPSPVVLNDKIMQLYGVKH"
     gene            1945..2934
                     /locus_tag="PKY63_12200"
     CDS             1945..2934
                     /locus_tag="PKY63_12200"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01420.1"
                     /translation="MKTIRLFIVTSAFCAASLLAPAQLTKQEYSDSQYSAQYNVSNGL
                     LHGKYVSYYANGIKRAQGKFSDNNRSGKWTVYDSTGKKQVVRKYQSLYSYKRIFPKPY
                     HKGPAKLLSEPVYAASNYSGAANTYFKLEKRHVVYAKRSWQFIDAGKNKMLFSTDTLM
                     ACLKTALKNDSVTVYSNSDDEFRTPLSGAETLKILNGNTNIAGFLIKEDEIFDNQRFL
                     TENRIIGLCPLVKDSNGQYRAIFWIYMPQFNKSLAHVKMQQPGLPRDIQTLEDVFFHR
                     FFHSSWVFSSSPYDRNLEEKLLLIDDNAQYTDKFIIGQIETEHDFWIQFFGND"
     gene            3048..4604
                     /locus_tag="PKY63_12205"
     CDS             3048..4604
                     /locus_tag="PKY63_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01421.1"
                     /translation="MTNLLTSNKSLGFSKGFILLILILVLLADRAISLYYFGFIYTDI
                     DQLILWNGAVDYSHGIFHEPFFYGQPYNYMLESVIAVPLLHLNVPVYQALPAATSLIA
                     TLPFIGLALFLHKKGKFFWACLCLVLPVLLPLEYNFLTSISRGFVQAHLFIPLLFIPL
                     FNPENKKNITLLFIASSICFIANQSSILLIFPIFMYVASFHYKSIYFYRNALWVFPFF
                     VLDFIFKFYYKIHPEKVLHNISGVRLDGKKFIESISSSNTFENLLPFTTAGGFYYPLM
                     FMILAIIAYKKGAKKELLFIFSAIFIILISFAVPKVQDPYPLENAGIFFSSSRFYLLI
                     PLLLIISAFMVLRNYKVKSYVSFILIAICSFSVFIKNMPIQETIDKTVKETSFPIAKN
                     QDLVSRAVNLKTIISENNVDLIVFGMSYGWNWNNLFDSYALYPLVIDRMHQTKKPIAV
                     NITGDRRSWQYENSESSNRILLIDTYIEEQLLKTLDYDTIGNSYILIRNNKTNTSNLF
                     EKLNCKFGNS"
BASE COUNT         1450 a         1044 c          882 g         1415 t
ORIGIN      
        1 ccgttcagct cattcctcat tcccggcatt gttttgttcg tggtaaatgg cgttctgaat
       61 atttcagccg ggattctgac cattcgcaat cacgcagcag cagcgcgttt cgtgatgttg
      121 caaggcctgc tgctctgcgg ctggattgtg gtgcagatcc tcatggtgcg cgacatcaat
      181 gcattgcata tcgtcatgtt cagcatcggg gttggtctga ttgggctggg atagttgcga
      241 accagagaaa aattgtaaat ttgttttatg gaaaacaaag aactcagatg gaaacagcgc
      301 tatcagaatt ttgaaatggc tttttcaaaa ttacaggaag caatgcagca aaacgaactg
      361 aatgaattgg agcgaaacgg cctgatccaa cggtttgagt ttaccatcga cctttcctgg
      421 aaagttttaa aagattatct cgaagaaaaa ggttttgttt ttaaaccttc gcccaaagac
      481 accatccggg aagcacagcg cgccggactg attgatttcg gacaggctct gattgacggg
      541 attgatatac gcaacgattt gtcgcacgat tacagcggaa ataaatttga acaatccgaa
      601 accattttgc gtgacacggt gtttcccgca ctgaatcaac tgaatcagtt tttcattctt
      661 gaagatgcca caaaataata tctcatagtc atggaaaaca ctgcagaatt attcggcttg
      721 tcgcccagag atattcaaac aatccatgat gttttttcga aatacaccga cattgaaact
      781 gtccggattt tcggcagccg cgccacaggc tctttcaagc ccgggagcga cattgatctt
      841 gccattatga attctgtgcc atcacgaata atgcttaatc taattaacga ttttgaagaa
      901 agttccctgc cctttaaaat cgatctgatt gacttcagtt ctttaaccaa tcccgcatta
      961 acagagcata ttcagaaggc tggcattgtg ttttacaacc gaccttcatc aaaaacagaa
     1021 aatcccggct gaaaatttat tccgatcatt accagacaat attgttcgtc cctcgtcgtt
     1081 tttattacca aataataaca gaacttaaaa tgataacgta ccgagtattt atacggactt
     1141 tggttttgct gttcttactg atatccggcc tgaatgccgg caaaatttct gcgcaaaaca
     1201 cttcagctgt cactattccc gaaagccgct tctacccagc gctgcatcga tacagttcta
     1261 ttcctgccga ttcgtcagtc gttgtacatt ctttcgaaac aacacccttt attacgcttg
     1321 gcgaatataa agaattcatg aaatccgttt ccggaagcga agaattaccg gattcgctca
     1381 tgcccgatac cagcatctgc attccgatga aatacaaaaa atacttgtcc ggaaaaaaat
     1441 atgagaagta tccggccctg ggaatttcat ggaaaaacgc gattgaatac tgtatctgga
     1501 gaacaaaaaa ggaaaatccc gacagcggaa ttgtttttat ctaccggctt ccatataaaa
     1561 ctgaatggat acaaatggct cagtatgccg actcagcact gaaaaaattt gaaatcaacc
     1621 gtcattatgc cgaatggatt atggcgtcgt ttgatgaatc cgccctctct ttcccgggaa
     1681 atctcaacaa cgcttacact ttcgaatatt tgtatctggc aggcaaatct gatccacagg
     1741 ttttaaagcg gatgtgcatc tatggcaaat catttctctc caaccaccaa aaaaacctgt
     1801 atggatattc cgatcagggc tatcgcgacg tggggttccg catggtgaag gaatatgtaa
     1861 acgtgcctgg acgaaagcca tcgcctgtag tgctgaatga caaaataatg caattgtacg
     1921 gagttaaaca ttaatcatca accaatgaaa acaatcagat tatttatagt tacatccgca
     1981 ttttgtgctg catcgttatt ggccccggca cagctcacaa agcaggaata cagtgactca
     2041 caatactcag ctcagtataa tgtcagcaat ggattgctgc atggaaaata tgtttcgtat
     2101 tatgccaacg gcatcaaacg ggcgcaggga aaattttccg ataacaaccg ctcaggcaaa
     2161 tggactgtgt atgatagcac cggcaaaaaa caagtggtcc gtaaatatca gtccctgtat
     2221 agctacaaac gaattttccc aaaaccatat cacaaaggtc ctgcaaagct gctttcagag
     2281 ccggtttacg ctgccagcaa ttattccggc gcagcaaata catactttaa attagaaaaa
     2341 agacatgtcg tttatgcaaa gcgttcatgg cagtttattg atgcaggaaa aaacaaaatg
     2401 cttttcagca ccgacacgct gatggcctgt ctgaaaaccg cactgaaaaa cgattccgtt
     2461 actgtctatt ccaacagcga cgatgaattc cggacaccgc tttcaggtgc tgagacgttg
     2521 aagattctga acggaaacac caacattgca ggcttcctga ttaaagagga tgagatattc
     2581 gacaaccagc gatttcttac agaaaaccgc atcattggcc tgtgcccgct ggttaaagac
     2641 agcaacggtc aatacagggc gatcttctgg atttacatgc cgcagtttaa taagtcactc
     2701 gcacacgtaa aaatgcagca acccggtctg ccacgcgaca ttcagacact cgaagatgtt
     2761 ttcttccatc gcttctttca ttcatcatgg gtgttttcaa gcagtccata cgaccgcaat
     2821 ttagaagaaa aactgctgct gattgatgac aatgcgcagt ataccgacaa attcatcatc
     2881 ggtcagattg aaacggaaca cgatttctgg atacagtttt tcggaaatga ctaatagtat
     2941 tctggaaata aaacaacaga ctacacctta ctaaacctat gcagactgaa taataatcaa
     3001 agagctgggt gctcagataa tttagtatat tgcactttga tttttttatg acaaacctat
     3061 taacaagcaa taaaagcctt ggtttttcaa aaggatttat cctgctgatc ctgatcctgg
     3121 ttcttcttgc agacagagcc atatcccttt actattttgg attcatctat actgatattg
     3181 accagctgat tttgtggaac ggagccgttg attattcaca cggcatcttt catgaaccgt
     3241 ttttttacgg acaaccctat aactatatgc tcgaatctgt aatagcagta cctcttctac
     3301 acttgaatgt tcccgtttat caggcgctcc cggcggccac cagcctcatc gcgaccctgc
     3361 cttttattgg tcttgctctt tttcttcata agaaagggaa atttttctgg gcctgtttat
     3421 gtctggttct gcctgtactt ctgcctttgg aatacaattt tctgacatcg atatcaagag
     3481 ggtttgtaca ggcacattta tttatcccgc ttcttttcat tcccttattc aatccggaaa
     3541 acaaaaaaaa tataacactg ttgtttatag catcatccat ttgtttcatt gcaaatcaaa
     3601 gctcaatatt actgattttt ccaatattca tgtatgtcgc ttcattccat tataaaagca
     3661 tttacttcta tcggaatgcg ctttgggtat ttcctttctt tgtccttgat tttattttta
     3721 agttttacta caaaattcat cctgaaaaag tattgcataa tatcagcgga gtcagattgg
     3781 atggcaaaaa atttatcgaa agcatcagta gctcaaatac atttgagaac ctgctcccat
     3841 tcaccactgc aggcggattc tactatcccc tgatgtttat gattctggca ataatagcat
     3901 acaaaaaagg agcgaaaaaa gaattactgt ttattttttc cgccattttt attattctta
     3961 tttcttttgc agtcccaaaa gttcaggatc catatcctct cgaaaatgcc ggcatctttt
     4021 tctcatcaag ccgattctat ctgctgatcc cgcttcttct aattatatcg gcctttatgg
     4081 ttcttagaaa ttataaagta aaatcctatg tctccttcat tctgattgca atctgttctt
     4141 tttctgtatt tataaaaaac atgcccattc aggaaactat tgacaaaaca gtaaaagaaa
     4201 caagttttcc cattgcgaaa aatcaggatc ttgtttccag agccgtcaat ttaaaaacaa
     4261 taatttctga aaacaatgtt gatttaattg tattcggaat gtcatacggg tggaattgga
     4321 acaacctgtt cgacagctat gcattgtatc cattggtaat tgacaggatg catcaaacaa
     4381 aaaaaccaat agcggtaaac atcactggcg accggcgatc ctggcaatat gaaaattcag
     4441 aatccagcaa ccgcattttg ctgatcgata cttacattga agaacaactt cttaaaaccc
     4501 ttgattatga tactataggc aacagttata tattaatcag aaataataaa acaaatacaa
     4561 gcaacctatt tgaaaaatta aattgcaaat tcggaaattc ctgaatcgtt ttattggaaa
     4621 taaataattg cgaaaattgc attctacagt gtaaaagaaa aatctattat tcacattgct
     4681 tttgattctc cattcacatc tgatcgcttg aaatcatctc tttcataatt tcaatagccg
     4741 cccccaccga ttgtatctta tccactttca ttatcaggcg ctcgttgtgc t
//
LOCUS       DAOPKE010000147         4763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_19462_length_4763_cov_10.403356, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000147 DAOPKE010000000
VERSION     DAOPKE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19462_length_4763_cov_10.403356"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PKY63_12210"
     CDS             <1..161
                     /locus_tag="PKY63_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPF01422.1"
                     /translation="KVDGLKLEFGHEWVHLRKSNTEPIIRIYTESRTEADADALAKKF
                     VELIERNA"
     gene            321..497
                     /locus_tag="PKY63_12215"
     CDS             321..497
                     /locus_tag="PKY63_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01423.1"
                     /translation="MKKKRTKKKKPNLLLDYVKTVKKLNREAMLESGAKPIERVVKSK
                     KVYTRKKKHRGGEE"
     gene            871..>2008
                     /locus_tag="PKY63_12220"
     CDS             871..>2008
                     /locus_tag="PKY63_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01424.1"
                     /translation="MKKNLFNTGKLPWWLIGVSMMMGGGIITEPQMISAALAEGNLSG
                     MWLMWSTLFGGAVGLSFFAHLWRRVPVKTENEFLLFRFSGRGSRILHAFRAVYVGGFL
                     VTFILAFTYIGLSNFMITAFGLTKAEAILIITCIVIVAAFFNSLGERIRLDFVFLIIF
                     AALFIVVMSALISDAGGMVHVGNTIANAGLDFSLFPETGTQAFNAFLVFVLLQWWMAS
                     ICDMPDMSGQKMMSARSPKDVVRSNVLPGIIAVIIGLFQFMLPFIAYADGYFASGLKP
                     ELAYISVFTNHLGFPLLIAAALMFLIAFLSVILNNLNWGGSLLIQNFYRYHVRPKATD
                     EQLSRAGLTTMIATAVAAGVVAYFSDTLFGFARYLFTIAAGVGPV"
     assembly_gap    2009..2108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2109..2681
                     /locus_tag="PKY63_12225"
     CDS             <2109..2681
                     /locus_tag="PKY63_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01425.1"
                     /translation="LFGFARYLFTIAAGVGPVFILRWYWWRINAWSQFSAMVAALVLP
                     NLFELLMYISTDFNAAIMQACAALELDQYSFTIIFLSAIVTCIWLSVTFLTKPDDEET
                     IKRFAEQVHPGGFWPAKYKKGRVFSGARLLSWILYAGRGITIYLAYWSFVNADYWLAA
                     MWTVVFLGLLFVVYRVLNSVNERAEEVESL"
     gene            complement(2833..4611)
                     /locus_tag="PKY63_12230"
     CDS             complement(2833..4611)
                     /locus_tag="PKY63_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPF01426.1"
                     /translation="MELTRQQNDILNATGNIRINAVAGSGKTTTVIEYARRIPKNKTI
                     LYLAFNKSVKMEAQKRFNECGLQNVRIETAHSLAFSHVVASSHYKVKANGYQTSELVQ
                     LLQIRSIGDALTEFIVANHVARFMSYYCNSEKKSLKELDYISVITDNKARDFVKKHYR
                     LIEAKTALFLNLMNDAKIEVTHDFYLKKFQLSHPVFRYDYILFDEGQDASAAMLDVFL
                     RQKAVKVIVGDTNQQIYGWRYAVNSLEKVDFQNYYLSESFRFGNDIALLATEVLKWKN
                     HMIDFNPVAIRGMGKQTTVKTRAVLARTNIGLLLKAIEILRGPKRINHIYFEGNFNSY
                     TYADEGASLYDVLNLHLGKIHLVRDPMIARMCCMEDLEDYIEKTEDKQLSMMVDIVKK
                     YGDEIPAILREIKAKHLPDQLRHKADVIFSTVHRCKGMEYDSVELVDDFYTEDKFKSC
                     TIPEKEEFRRELMLSEINLLYVAVTRARCELHIPESLIPSGFPVSKNIHVIAKKTKTD
                     AISVDDWRKITYRKSFPQTQDEAIAWVRARENHHAAYRSWTRDMDDELRQMFNNGDTL
                     SDIAKQTGRSKGAIMARMRLLELLDD"
BASE COUNT         1190 a          953 c         1094 g         1426 t
ORIGIN      
        1 ataaagttga tggactgaag ctggaattcg gacacgagtg ggtgcatctg cgcaagtcga
       61 acaccgagcc gatcatccgc atctacaccg aaagccgcac cgaagctgat gccgatgcat
      121 tggcaaaaaa gtttgttgag ctgattgaga ggaatgcata gagtgctgtt caacgctgag
      181 aggtcctcct tcggcggacg cactcaggtt gaaccagagc tgatatcgaa acatctcatt
      241 ccatcgtcag ccacatcaac tttctgtgcg atcggtgcag acatggaatc gtgatgtttt
      301 cgccatcaaa gacaaaaaaa atgaaaaaga aacgcaccaa aaagaagaaa ccgaatttgc
      361 tgcttgatta cgtgaagacc gtgaaaaagc tcaaccgcga agccatgctc gaatcaggcg
      421 caaagcccat tgagcgggta gtgaagagca agaaggtata tacaagaaag aagaagcaca
      481 gagggggaga ggagtaggtt aaatagaaat attttttcct ttaattaaaa aaacctgttg
      541 ctttttcggt ttttgcagca ttgtcttccg gaatatttta cacgatttac atgttttaag
      601 cgcaaaacag tatctttata ttattgttat acagttaatt gtatttgcaa taaaatgcaa
      661 ttgtatatat atgttagttg ctatatattt gtatcgttta aatacatgac cctatagaca
      721 gaatatatac tatagtttat tcctatacac aagcgattct gatttccccc tgcatctgcc
      781 cgttgaaagc gggcagaaag ccggggagat cagtattttt ttatagaatg caataaaaga
      841 taaatactac ttttgcgagg ctgctgttat atgaagaaaa accttttcaa taccggaaaa
      901 ctgccctggt ggctcattgg agtgtcgatg atgatgggcg gcggtattat tactgaacca
      961 cagatgattt ctgctgcact tgccgaaggc aatctgtcgg gtatgtggct catgtggtcg
     1021 acgctattcg gcggtgctgt cgggttgtcg ttttttgcgc atttgtggcg cagagtaccg
     1081 gtgaaaaccg agaacgaatt tttactgttt cgcttttcgg gccgcggatc tcggattctg
     1141 catgccttca gagccgttta tgtaggcgga tttctggtta cttttattct tgcgtttacg
     1201 tacattggtt tgtcgaattt tatgattacc gcatttggcc ttacaaaagc cgaagcgatt
     1261 cttatcataa catgcattgt gattgttgca gcattcttca acagtttggg tgaacgcatc
     1321 cgtctcgact ttgtttttct gatcatattt gctgcgttgt ttattgttgt aatgtcagca
     1381 ttaatcagcg atgctggcgg tatggtgcat gtcggaaaca ctattgccaa cgcaggactt
     1441 gatttttcgt tgttcccgga aacaggcaca caggcattca atgcatttct tgtatttgta
     1501 ttgctgcaat ggtggatggc ttcgatatgt gatatgcccg atatgagcgg gcagaaaatg
     1561 atgtcggcac gcagcccgaa agatgttgta cgaagcaatg ttttgcccgg aattattgca
     1621 gttattattg gattgtttca gtttatgctg ccgttcattg cctatgccga cggatatttc
     1681 gcaagtggct tgaagccaga actggcctac atttcggtgt tcacaaatca tcttggtttt
     1741 ccattgctga ttgcagcggc actgatgttt ctgattgcat ttctgtccgt gattctcaat
     1801 aatctgaact ggggcggatc gctgctgatt cagaatttct accgctatca tgtgcggccc
     1861 aaagccaccg acgaacagtt gagcagagcc gggttgacaa ctatgattgc aactgctgta
     1921 gccgcaggag tggtggcgta tttttccgac accttatttg gatttgcgcg ctatcttttt
     1981 accattgcag cgggagtcgg accggtttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnntt atttggattt gcgcgctatc tttttaccat tgcagcggga gtcggaccgg
     2161 tttttatatt gcgctggtac tggtggcgca tcaatgcgtg gtcgcagttt tcggccatgg
     2221 tggctgcgct ggttctgccc aatctgtttg aattactcat gtacatttca acggatttca
     2281 atgctgcgat tatgcaggcc tgtgctgcct tggagctcga tcaatattca tttacaataa
     2341 tatttttgtc ggcaatagta acatgtatct ggctttcagt cacttttttg accaagcccg
     2401 acgatgagga aacgataaaa cgatttgcgg aacaggttca tccgggcggc ttctggcctg
     2461 cgaaatacaa aaaaggtcgt gttttttccg gcgcccgatt attgtcctgg atattgtatg
     2521 caggccgtgg catcacgatt tatttagcgt actggagttt tgtaaatgct gattactggc
     2581 tggctgccat gtggacagtg gtctttttgg gattgctgtt tgttgtgtac cgggtgctga
     2641 attcggtgaa tgagcgggct gaagaagttg aaagtttata aagtttcaag tttataaaga
     2701 tgacgttaga gcaggcggca tgcggtttaa ggcacaatgt tctatgcacg acgcctaacg
     2761 cacaacgcat ggagcatggc ttcgcttcgc tctggctttc agaataaaac tttttgcagc
     2821 ggggcaggga agtcagtcat caagtagttc aagcaatcgc atccgagcca tgatggcgcc
     2881 tttggagcgt ccggtttgtt ttgctatgtc agaaagtgtg tcgccgttgt tgaacatttg
     2941 tctaagttca tcatccatgt cgcgtgtcca ggagcggtac gcagcatgat gattctcgcg
     3001 tgcacgcacc catgcgatag cttcatcctg ggtttgtgga aaggatttgc ggtatgtaat
     3061 tttgcgccag tcatccactg aaatcgcatc ggtcttggtt ttctttgcaa tgacgtgaat
     3121 gttttttgat acagggaagc cagaagggat gagcgattca gggatatgga gctcgcagcg
     3181 tgcacgtgtg acagccacat ataacagatt gatttcggag agcatcagct cccggcgaaa
     3241 ctcctctttc tcagggattg tgcaggattt gaacttgtct tcggtgtaaa aatcatccac
     3301 cagttcaacg ctgtcgtatt ccattccttt gcagcgatgc actgttgaaa atatcacatc
     3361 ggctttgtgc cgcagctgat caggcaaatg tttcgctttg atttcgcgca ggattgccgg
     3421 aatttcatcc ccgtattttt tcacaatgtc caccatcatg gacaattgtt tatcctcggt
     3481 tttttcgata taatcttcca ggtcttccat acaacacata cgtgcaatca ttggatcgcg
     3541 caccagatgg atttttccaa gatgcaaatt aagcacatcg tacaatgaag caccttcatc
     3601 ggcatatgtg tagctgttga aatttccttc aaaataaata tgattgatcc gtttggggcc
     3661 acgcagaatt tcgatggctt tcagaagcag gccaatgttg gttcgcgcca gtactgctct
     3721 ggtttttaca gtagtttgtt ttcccatacc gcgtattgcc accggattaa aatcaatcat
     3781 gtgatttttc catttcagca cttcggttgc caacaatgcg atatcgtttc caaagcgaaa
     3841 actttcggat aaatagtaat tctgaaagtc caccttttca agtgagttca ccgcatagcg
     3901 ccagccgtag atctgctgat tcgtatcacc gacaatgact ttcactgctt tctgcctcaa
     3961 aaaaacatcg agcatggcgg ccgacgcatc ctgaccttca tcaaacagaa tataatcata
     4021 tctgaacacc ggatgcgaaa gttgaaattt cttcagatag aaatcgtgcg taacctctat
     4081 tttcgcgtca ttcatcaaat tgaggaacag cgcggttttt gcttcaatta aacgataatg
     4141 ttttttgacg aaatcacgtg ctttgttgtc ggtaatgacg gaaatgtagt ccaactcttt
     4201 caatgatttt ttttcgctgt tgcaataata tgacatgaat cgcgcgacat gatttgcaac
     4261 aataaattca gtgagagcat caccgatgga tctgatctga agcagctgga caagctcgga
     4321 tgtctgatag ccattagctt tcactttgta gtggctggaa gcaacaacat ggctgaatgc
     4381 cagtgagtga gcagtttcga tgcgcacatt ctgcagaccg cattcattaa agcgtttctg
     4441 tgcctccatt ttcaccgatt tgttgaaggc cagatacaga atggttttgt ttttcggaat
     4501 gcgacgggcg tattcgatga cggtggtggt ttttccagag ccagccacag cattaattct
     4561 gatattgccg gtggcattca gtatatcgtt ttgttgcctc gttaattcca taatgttcaa
     4621 attctcctga caaaggtaaa aaatgtattt ttaaaaaact gcataaatac ttagctattt
     4681 tcacttttga ttaataatca gtcatatagt ttgattagat atataacagg aagcttatag
     4741 tataatactt ataataaaaa gac
//
LOCUS       DAOPKE010000148         4569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_20662_length_4569_cov_12.151086, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000148 DAOPKE010000000
VERSION     DAOPKE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20662_length_4569_cov_12.151086"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PKY63_12235"
     CDS             complement(<1..2008)
                     /locus_tag="PKY63_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01427.1"
                     /translation="MRSTLKKMSAMMLAFLMVVTMASVLAVSAELKTGARTLGLQEGT
                     TLGTSANYATLGTSMDGAFFADAIAVWGWNGITSYAPLSNQNGIDIFVGEGNFFDNSL
                     DGGFSLDSIKNIYIDLNAWSNPTDSSKWAKYGTKTITIGFNAWTDDDGNLVWAKVPAS
                     GADGSKMLQFFTFQVDAGVAQIQLFDTDTIENIENGGDNGIWNVYFQECTKTYTETNG
                     TATTADDFVIRGQVLIGRGYVRNFDAETALWLDLFHGTHEYTKLFGYDFSLEGQKSYN
                     TSGTNASVDFCLTAGSVDVTQWDVVSSQYVTTTKSLAGTSFDINLLKELLTTVSSPML
                     TDSIAAESKGFFWGTDTSIYNAFAYMFFLNMRYNSLGFNANTADPRIGTSTLVPSDAK
                     VYYEFGNLQGYLRLTWTTSNAVSGLQTTTASGGELGGQVETMWPVSDYLDGRLVANQT
                     QTLTASELEAGKVLLWEYMTYTSNYQPFGDSFSYPDNQNDYAAFIYETSRGTQSDWNF
                     DTKIVALAADMNLVDAGGKVDLQLVPTIGGADLVAGLSETDKAGKSLRDYGYTLNYYV
                     DYTTDFMTQMTIDIHDKTLNDITTAYGTANFVVSIAKGAIVSAYSCTITQYDETFVIP
                     LQDGTFTLMRIKVKTNYDDAVSPLADSDITDFNYSRLTFVEQIKL"
     gene            2257..2901
                     /locus_tag="PKY63_12240"
     CDS             2257..2901
                     /locus_tag="PKY63_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPF01428.1"
                     /translation="MNYNRFIVSLSFYRERQMKINKNKKLYSVFETYLITIQALRTMP
                     QLQRAKKKGDLDDSFIERIMLAVTEVNGCAICSYAHTKIALEAGLTDVEIKNMLSGLS
                     ADVPENEIPAVLFSQHYADTRGYPSKDTWQRIVEIYGLSAAKGILGATRAIMMGNAYG
                     IAWSSFFNRLRGKPDPRSGFLYEISMIFFGFLFFPFAIIHALLLNLFKVSLIHF"
     gene            complement(2977..3252)
                     /locus_tag="PKY63_12245"
     CDS             complement(2977..3252)
                     /locus_tag="PKY63_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HPF01429.1"
                     /translation="MYEDKTLICKDCGAEFVFTAGEQEFYATKGFVNEPQRCKSCRDN
                     RKNAARGEREMYDCVCAECGAPCKVPFKPRDDRPVYCSDCFAARKGN"
     gene            complement(3502..4206)
                     /locus_tag="PKY63_12250"
     CDS             complement(3502..4206)
                     /locus_tag="PKY63_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01430.1"
                     /translation="MKKFIMTTLTMLLIACFGCSYVSTPVISEESQPNSSVASEVSDE
                     TSSVGSDLNLPSCEFCLTTEYTFEVNVMTTSDWQSYEDSAGSETKTFALTFPENWIRD
                     ATDAPTFHDQYTDIKIFESIAAVKLPAGFDFAGSFIKENFEDSMSDTTVIGDVTIGTL
                     ALSNGERTYAQVVQSVVPEGGGEIQIDRWYPYFCVIVDGDYAYCVQFYSLDDPTSDDM
                     NAGLFKEIMNTFRPIS"
     gene            complement(4208..>4569)
                     /gene="dapB"
                     /locus_tag="PKY63_12255"
     CDS             complement(4208..>4569)
                     /gene="dapB"
                     /locus_tag="PKY63_12255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPF01431.1"
                     /translation="DIEIVEAHHNKKVDAPSGTALMLAKSVSEGLKETPVYTYDRHLQ
                     RKPRDHEEIGIHSIRGGTIIGEHSVIFAGEDEVLTFSHSAGSRKMFAAGAVNAALFLI
                     HKKPGMYDMNDLIGGKI"
BASE COUNT         1228 a         1052 c         1011 g         1278 t
ORIGIN      
        1 acagcttgat ctgctcaaca aatgtcagac ggctgtagtt gaaatcggtg atgtcgctgt
       61 cggccaacgg gctgactgcg tcgtcatagt ttgtcttgac cttgatgcgc atcagtgtga
      121 atgtgccatc ctgcagcggg atcacgaagg tctcgtcata ctgggtgatg gtgcagctgt
      181 atgcgctgac aatggcgccc tttgcgatgc tgacgacgaa attcgctgtg ccgtaagcgg
      241 tggtgatgtc attcagggtc ttgtcatgga tgtcgattgt catctgggtc atgaagtctg
      301 tggtgtaatc gacatagtag ttcagcgtgt aaccgtaatc gcgcagcgat ttgcccgctt
      361 tatcggtttc gctcaggccc gcaaccagat cggcgccgcc gatggtcgga accagctgga
      421 ggtcaacttt accgcctgcg tcaaccaagt tcatgtctgc tgccagagca acgatcttgg
      481 tgtcgaagtt ccagtcggac tgtgtgccac ggctggtctc gtagataaag gcagcatagt
      541 cattctggtt gtcgggataa gagaaagaat ctccgaaggg ctggtagttg gatgtatatg
      601 tcatatactc ccagagcaga accttaccgg cttcaagctc tgatgcggtc aaagtttggg
      661 tttggttggc aaccaagcgt ccgtcgagat aatccgatac cggccacatc gtttcaacct
      721 gaccgccaag ttcaccgccg gaggcagtgg tggtctgaag accggaaacc gcattggaag
      781 ttgtccaggt caaacgcaga tatccctgaa gattcccaaa ctcataataa actttagcat
      841 cggacggaac caatgttgaa gtaccgattc tcggatcagc ggtatttgca ttgaaaccca
      901 gcgagttgta tctcatatta aggaagaaca tatacgcaaa tgcgttgtat atacttgtat
      961 ctgttcccca gaagaacccc tttgactcag cagcaattga atctgtcaac attggacttg
     1021 aaaccgttgt cagtaattct tttaataaat tgatatcaaa agaggtgcct gccaagctct
     1081 ttgtggttgt tacatattga gatgaaacaa catcccactg agtaacatca acgctgccgg
     1141 ctgtcaaaca aaaatcaaca gaggcattgg tgccgctggt attatagctc ttttgtccct
     1201 caagactaaa atcatatccg aacagttttg tatattcatg tgtgccatgg aataagtcaa
     1261 gccaaagtgc tgtctctgca tcgaaattgc gaacataacc gcgtccgatc agaacctgac
     1321 ctctgatcac gaaatcgtca gctgtcgtgg ctgtgccgtt tgtttctgtg taggtcttgg
     1381 tgcactcctg gaaatacaca ttccagatac cgttatcgcc gccgttttca atgttctcga
     1441 ttgtatcggt atcaaacaat tgaatctgag caacgccggc atccacctgg aaggtgaaga
     1501 actgaagcat tttgcttccg tcagctcccg aagccggaac cttggcccaa accagattac
     1561 cgtcatcgtc agtccatgca ttgaagccga tggtgatggt ctttgtaccg tatttggccc
     1621 atttggatga atctgtggga ttggaccatg cattcagatc aatatagata tttttaattg
     1681 aatcgaggct aaagccgcca tcgagagaat tatcaaaaaa attcccttct ccgacaaaaa
     1741 tatcaatacc gttctggttg ctcagaggcg cataagatgt gatgccgttc cagccccaca
     1801 ccgcaattgc atccgcaaag aatgcgccgt ccattgatgt ccccaatgtt gcatagttcg
     1861 cggatgtacc aagagtcgtg ccttcctgta aaccgagtgt tctcgcgccg gttttaagtt
     1921 cggcggagac tgcaagcacg gaagccattg tcacgaccat cagaaatgcg agcatcattg
     1981 cagacatctt cttgagggtt gatctcatgt ttgttttttc ctcctaaaat ttttatgccg
     2041 ctgcagatgt gatttgaaag ggtgcagcgg cttccccatt tctcagggaa ttatacataa
     2101 aattttaaca aatgtcaatt gaaatcttat atttttactg ttttttgttg tattaatttc
     2161 gtctttctat ataaaattca aatctcctct tttagagttt ttttaacact ttttccttgt
     2221 ttttaatgta acagagtcac aaaaataatg cattttatga attataatag atttatagtt
     2281 tccttatctt tttatcggga gcgtcaaatg aagatcaata aaaacaaaaa attatactca
     2341 gtttttgaaa catatctgat tacgattcag gcattgcgca caatgccgca actgcaacgc
     2401 gccaaaaaga agggtgattt agacgattca ttcatcgaac gcatcatgct tgcagtgact
     2461 gaggtgaacg gctgcgccat ttgttcctat gcgcatacaa aaattgcgct ggaagccggt
     2521 ttgaccgatg ttgaaattaa aaacatgctt tcgggcctca gcgctgacgt cccggaaaat
     2581 gaaattccgg cagttctgtt ttcacagcat tatgccgata caagaggata tccctcaaaa
     2641 gatacctggc agagaattgt tgagatttac ggcctttctg ccgcaaaagg aatattgggc
     2701 gcaacacgag ccattatgat gggaaatgca tacggaatcg cctggagttc ctttttcaac
     2761 cgtctgagag gaaagcccga tccgagaagc ggatttctct atgaaataag catgatcttt
     2821 ttcggttttt tattctttcc tttcgcaatt atccatgccc ttctgcttaa cttatttaaa
     2881 gtgtcgctta ttcattttta aacgaatcat aaattttgta caatgcaaaa ggccccgctt
     2941 atagcggagc cttgaatgct gattcaggta attagattag ttaccttttc tcgcggcaaa
     3001 gcaatcactg caatacacgg gacgatcgtc acgcggcttg aaggggactt tgcacggagc
     3061 gccacactct gcgcagacgc aatcatacat ctcgcgctca cctctggcgg cattcttgcg
     3121 gttgtcacgg caggacttgc atctctgcgg ttcgtttaca aagcctttgg ttgcataaaa
     3181 ttcctgctca ccagctgtga acacgaactc agcgccacaa tctttgcaaa taagggtttt
     3241 gtcttcgtac atggattttc ctcgctttaa ataatttgcc ctgcggatac aacagcattg
     3301 acacccgtgt gaaacacacc aagggaacac attcgcattc gatcagtggc ataaaaacat
     3361 agtatcacaa aactctcacc ttgtcaacaa ccgagtgaag ttaacctgtt ttccgttgtt
     3421 aaaaacgcac atttttattg aaaaatgtgg cagtttttat gaattgtgca taaattgtca
     3481 aaaaatattc gtgcaacttt ttcaagaaat tggtctgaaa gtattcatga tttctttgaa
     3541 taatccggca ttcatatcat ccgatgtggg atcatccaag ctgtaaaact gcacgcaata
     3601 ggcataatcg ccgtctacaa tcacacaaaa atacggatac caacgatcga tttgaatctc
     3661 tcccccgcct tcgggaacga cgctttgaac aacttgtgca taagtccttt caccgtttga
     3721 aagcgccaat gttccgattg tcacatcgcc gataaccgtg gtgtcggaca tggaatcttc
     3781 aaaattttct ttgataaaac tgccggcaaa atcaaatccg gcggggagtt ttacagcggc
     3841 gatggattcg aatatcttaa tgtcggtata ttgatcatgg aatgtgggcg catcagttgc
     3901 atcacgaatc cagttttccg gaaaggtcaa tgcaaaggtc tttgtctcgg aaccagcgga
     3961 atcctcatag gactgccagt cggaagttgt catcacattg acttcaaatg tatactccgt
     4021 ggttaaacaa aactcacagg acggtaaatt gagatccgaa cccaccgaag acgtctcatc
     4081 ggacacttca gaggcaacgg atgaattcgg ttgagattcc tctgaaatca caggggtgga
     4141 aacataggag catccaaagc aagcaatcaa gagcatcgtt aatgtggtca taatgaattt
     4201 tttcatatta aatcttccct ccgattaaat cgttcatatc atacattcca ggctttttgt
     4261 gtatcaaaaa caaagcggca ttgactgcgc cggccgcgaa catcttacgg ctgccggcac
     4321 tgtgagaaaa agtgagaacc tcatcctctc cggcaaaaat gaccgagtgc tcgccgatga
     4381 ttgtgccgcc gcgaatgctg tggatgccaa tctcctcatg gtctcggggc tttcgctgta
     4441 aatgccgatc ataagtatag acgggcgttt ccttcaatcc ctcactcacg gatttggcca
     4501 gcatcagcgc agtaccgctg ggcgcgtcca cctttttatt atgatgcgct tcaacgatct
     4561 caatatcaa
//
LOCUS       DAOPKE010000149         4347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22093_length_4347_cov_12.525163, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000149 DAOPKE010000000
VERSION     DAOPKE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22093_length_4347_cov_12.525163"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..692)
                     /locus_tag="PKY63_12260"
     CDS             complement(24..692)
                     /locus_tag="PKY63_12260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPF01432.1"
                     /translation="MSTLNDYIEKFCLLNSTRTSPVLSAVYRDTWVNVMYPQMITNEL
                     QAHFLNMISLLVKPTQILEIGTFTGYTTICLASGLTEDGKIITIEKNAELETRIRKNL
                     ESAKLNDKTELLLGDATEILQSDFSNNIASFDIIYIDADKEHYPTYLNLAYPLLKPGG
                     IMLADNVFWGGKMFDENSKHTKESLGVKKFLSEAATLHWQSHTIMPLGDGLFFGIMPK
                     GNLQ"
     gene            complement(689..1909)
                     /locus_tag="PKY63_12265"
     CDS             complement(689..1909)
                     /locus_tag="PKY63_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF01433.1"
                     /translation="MAELQILKTGLPLIYIHRNQKIGHIVIHFNRGSSSDPEKLQGAV
                     HLLEHMLFKGTTTRNYFDLICGIEHFGADLNAFTTKENMIIHVSYPDQYMDDICLILA
                     DIVNNSIFDEKELKKEKQVVCDEIKSYRDTPEEFIYDEWERLMLSGNGLSHPILGSAA
                     SVNRISLDDLKKTYKSIRSDYQISVVSSRTKDHVKKIISKHFKTKVKSTRSVNLTKII
                     REEKPKIKILNDDVSQAHVILGRIGPHYTHDDQLPAYLLSSFLGGSAMSSVLNLELRE
                     KRGISYTIESSLQSYRDKGLFFTYFTCDKQRTGKALQVIAAIFENYRINGLSKKEFDT
                     LIRMTKSQYTMFFEPALNEAMYHSKFYQYYNQIVQIPDLMRELGKIKLEAVNTVAAKL
                     LDFEKFSRIIIEKK"
     assembly_gap    1972..2071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2072..2841)
                     /locus_tag="PKY63_12270"
     CDS             complement(<2072..2841)
                     /locus_tag="PKY63_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01434.1"
                     /translation="MMRFLYIQLLCVLLSSCSVRLMDKHVLQCASETSGLAVSEDTSQ
                     LYTIEDSGNGPHIYEISKDGKYLGRWDFMHRSNVDWEAMADDTNNYFIGDIGNNLRNR
                     DTLTIYQKLVASCPFDDMAKTRFICDHPLAPFDFEAMFFSNDSMYLITKNHREKYTYL
                     FRVEVNFELNNITPIDSARIRGQITGADYDAENNLLIMTGYRWYIPFVLIKKDATPST
                     ALCKPLKRKTFWLRPAVQTEAVVIVNPAEIYVSAEGNPL"
     gene            complement(2838..3584)
                     /locus_tag="PKY63_12275"
     CDS             complement(2838..3584)
                     /locus_tag="PKY63_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF01435.1"
                     /translation="MPTATDIKIFRSLKDKSSREEHGFFVAETPKVVSDLIAGGLSVK
                     VIYSLHDVLSDWQKQFPHLKIEGVSNKELERLSFLKQPHEVCAAFNIPEKKKFVQQAV
                     TLILDNISDPGNMGTIMRTAHWFGIKNIICTKNSVDIYNPKVVQSTMGSLARVHVHYC
                     SKEEIASMLEKNTTICGAFMDGENIFTTQINAPVAIIIGNEAHGVQSLGSLVQRRISI
                     PAHNADDHAESLNASVAAAIIMSMVSKFSE"
     gene            complement(3621..4220)
                     /locus_tag="PKY63_12280"
     CDS             complement(3621..4220)
                     /locus_tag="PKY63_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01436.1"
                     /translation="MKRFLSALVLIGLSLATLHAQTDEPKRQKGVRSSSNSFYINVLG
                     PSIPGSINYERIWTKNGVVNIGTKIGGFFISLPKVDELTIANGTAECTFLFGRSAHMF
                     EMGIGWAGHYGNYFSEDGGKTKHYAVPTSTFSMHYRYQKPKGGVFFHVGFTGSSILMF
                     GSTDLVELAAGNAAIYGFKGLTGEKPSFSLFSIGIGASF"
BASE COUNT         1136 a          862 c          928 g         1321 t
ORIGIN      
        1 ctggagtcat ccaaatcaat ggactattgt aaattccctt ttggcattat tccaaaaaac
       61 aacccgtcac caagcggcat aatggtgtgg ctttgccagt gcagggtagc ggcttcgctg
      121 agaaattttt tcacacccag agattctttg gtatgttttg aattctcatc aaacatcttt
      181 ccgccccaga aaacattatc ggccagcata atgccacctg gcttcagaag cggataggcg
      241 agattcagat atgttggata atgttccttg tcggcatcga tgtagataat atcaaatgat
      301 gctatattat tggaaaaatc gctttgaaga atttctgtgg catcgcccaa aagcaattct
      361 gttttgtcat tcagcttagc cgattcaaga ttcttgcgga ttctggtttc cagttcggcg
      421 tttttctcaa tcgtgattat ctttccatct tcagttaaac ccgaagccag acaaatggtc
      481 gtatagcccg taaatgttcc tatttcgaga atttgtgttg gtttaaccaa caacgatatc
      541 atattgagaa aatgagcctg cagttcgttg gtaatcattt gcggatacat cacatttacc
      601 caggtgtcgc ggtaaactgc gcttagcaca gggcttgtcc gggtgctatt gagcaaacag
      661 aatttttcga tataatcatt gagcgtgctc attttttttc tatgatgatt cggctgaatt
      721 tttcaaaatc cagaagtttg gcagctacgg tgtttacggc ttcgagtttg attttcccga
      781 gttcgcgcat cagatcggga atttgtacaa tctggttgta atattgataa aacttggaat
      841 gatacatggc ttcgttcagt gccggttcaa aaaacatggt gtactgactt ttggtcattc
      901 gaatcagggt atcgaattct tttttcgaca aaccattgat tctatagttt tcaaaaatgg
      961 cggcaatcac ctgcaaagct ttacctgtgc gttgtttgtc gcatgtgaag tatgtaaaga
     1021 aaagcccttt gtcgcgataa ctctgcagcg aggattcaat ggtatatgaa attcctcttt
     1081 tttcgcgcaa ttcgagattt aaaacggaac tcattgcgct gccaccaaga aatgatgaaa
     1141 gcagatatgc cggcaactga tcatcgtgtg tatagtgcgg acctatccga cccagaataa
     1201 catgtgcctg actaacgtcg tcgttgagga ttttgatttt gggtttttct tcacggataa
     1261 tttttgtgag attgacagat cgggtacttt ttacttttgt tttgaaatgt tttgaaataa
     1321 tcttttttac atgatctttt gtacggctac tgactacaga aatctgatag tcgcttcgta
     1381 ttgatttgta tgtttttttt agatcatcaa gcgaaatgcg gtttacagag gccgctgatc
     1441 cgagaatcgg atggctgagc ccattgcctg aaagcatcag ccgttcccac tcatcgtaaa
     1501 tgaattcttc gggtgtgtcg cggtatgatt ttatttcatc acatacaacc tgtttttctt
     1561 ttttcagttc tttttcatcg aaaatggaat tattgacgat atcggcaaga attaaacaga
     1621 tgtcatccat gtactgatcg ggataggaga catgaataat catgttttct tttgtagtga
     1681 aggcattcag gtcggcgccg aaatgttcaa ttccacaaat caaatcaaaa tagttacggg
     1741 ttgtagtgcc tttaaacagc atatgctcca ataaatgaac agcaccctgt agcttttccg
     1801 ggtctgatga tgagccccgg ttgaaatgaa taacaatatg cccgattttt tgattccggt
     1861 gaatgtatat caggggtaat cccgttttta aaatctgtag ttcagccatt tatttaaagg
     1921 catttcgaaa ttgtttgcaa atgtacatgt ttgtattgaa cagcatgcat tnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcgggttt ccttcggctg aaacatatat
     2101 ttccgccggg tttacaatca ctacagcttc ggtttgaaca gcaggccgca accagaatgt
     2161 ttttcgcttc aatggcttgc agagcgcggt tgaaggtgtt gcatcttttt taatcagcac
     2221 gaacggaatg taccagcggt agccggtcat tatcagcaaa ttgttttcag catcataatc
     2281 agctcctgta atctggcccc ggatgcgggc tgagtcgatg ggggtgatgt tgttcagttc
     2341 aaaattgact tcaaccctaa aaaggtacgt gtatttttct ctgtgatttt ttgtaatcag
     2401 atacatgctg tcgtttgaaa aaaacatggc ttcgaaatca aaaggagcca gcggatggtc
     2461 gcatataaaa cgagtctttg ccatatcatc gaaaggacag gaagcaacca gtttttgata
     2521 tatagtcagc gtgtcgcgat tgcgaagatt gttgccgata tcgccaatga aatagttgtt
     2581 tgtgtcatct gccattgctt cccaatcaac gtttgaacga tgcataaaat cccatcgtcc
     2641 aaggtattta ccgtctttgc ttatttcata aatatgcggg ccattcccac tgtcttcaat
     2701 ggtgtacaat tgacttgtat cctcagaaac tgccagaccg gatgtttcgc tggcacattg
     2761 caaaacatgt ttatccatga gccggacgga gcatgaagac aacagaacgc aaaggagctg
     2821 aatgtacaga aaccgcatca ttcactgaat ttggacacca ttgacataat gatggctgct
     2881 gcaactgatg cattgagcga ctccgcgtga tcatcagcgt tatgggcagg tatcgaaata
     2941 cggcgctgca ccagcgatcc gagactttga acaccatgcg cttcgttgcc gatgataatg
     3001 gcaacaggag catttatctg cgttgtaaag atattttctc catccataaa agctccgcag
     3061 atggtggtgt ttttttcaag catggatgca atttcttcct ttgaacaata atgcacatgt
     3121 actctggcca ggcttcccat ggtgctttgt accacttttg gattatagat atcgacactg
     3181 tttttggtgc agataatgtt ttttattccg aaccaatgcg ctgtacgcat gatggtgccc
     3241 atgtttccgg ggtcgctgat gttgtcgagt attaaagtaa ctgcttgctg aacgaatttt
     3301 ttcttttccg gaatattgaa cgctgcgcac acttcgtgtg gttgtttcag gaatgaaagc
     3361 cgctccagtt ctttgtttga aacgccttcg attttcagat gcggaaattg tttctgccag
     3421 tccgataaaa catcatgcag cgaataaatg actttgacag ataatcctcc tgcaatcaga
     3481 tctgaaacta cttttggtgt ttcagcaaca aaaaatccat gctcttcacg cgaagatttg
     3541 tcttttaggg aacgaaatat tttgatatct gtagcagtgg gcatgatgca aagatagtga
     3601 aattcaattt ttcgaaaaaa tcagaaagat gccccaatgc caatcgaaaa cagactgaat
     3661 gaaggtttct ctccggtcag tcctttgaat ccgtaaatag cggcatttcc tgcagccaat
     3721 tctaccaggt cggttgagcc gaacataaga atggatgatc ctgtgaaccc gacatgaaag
     3781 aacacgccac ctttgggttt ctgataacga taatgcatgc tgaatgtact ggtgggaacg
     3841 gcgtagtgtt ttgtttttcc tccatcttcc gaaaagtaat ttccatagtg tccggcccag
     3901 ccaatgccca tttcgaacat gtgcgccgaa cgtccgaaca gaaaagtaca ctctgcggtg
     3961 ccgttggcaa tggtcagttc atcaactttc ggaagactga taaaaaaacc gccgatttta
     4021 gtgccgatat tcactactcc gtttttagtc cagatgcgtt catagtttat tgatcccgga
     4081 atgctgggcc caagtacgtt gatgtaaaaa ctgttactgc tcgagcgaac ccctttctgg
     4141 cgctttggtt cgtcggtttg agcatgaaga gtggcaagtg atagaccaat caaaacaagt
     4201 gcggataaaa atcttttcat gggggttggt ttggaaaagc aaatatagca cttgggtagc
     4261 gcaaatacaa attcattttt tttcatggaa accattccgt acctcacggt acgggattgt
     4321 gttgttctgt tttctaccaa tatgttg
//
LOCUS       DAOPKE010000150         4332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22195_length_4332_cov_10.651157, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000150 DAOPKE010000000
VERSION     DAOPKE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22195_length_4332_cov_10.651157"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="PKY63_12285"
     CDS             <1..1523
                     /locus_tag="PKY63_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPF01437.1"
                     /translation="GSFAMEAGCNDVTTCFSLSSTPATNFTVNYTILGTATNGVDYPT
                     IPSSLTIPAGSDSVCITISPTMDNLAEGVETIMLVLQNQISCNTVSDTVEIMILDYEH
                     VSTLMSPDTSICGDTVNIWALGTLGVPPYNYAWNNGLPSTTSHLVSPTTTTTYVVTVT
                     DYCNHSTTNNVTITVGGSFVDAGPDQTICEGQSATLNAASDNSIVWNTGDTTLSITVS
                     PPHDSTYVIIAGSGDCMSSDTVTVFVLPQPDISASVFPDRICEGKSAQLTATGADMFL
                     WESNPFDATLNQTPGGLNNMLDITPSSTTTYNVTGTNVGGCFDTVTVKITVVPAPVAS
                     FFTQPPYASSFNPTIQFFDNSQGNPVLWRWLLSDGSTYDIPEFTHTYPMDTWGEFPVL
                     MYVENADGCSDSITQMITIKPDYTLYIPNAISPNGDGINDRFHISGLNIPIKDFSIRI
                     YDRWGALMFSSSVPSFEWDGYVDGKVVPDAIYNYRLIFSDPTGNVHIRNGSITIIY"
     gene            1892..2902
                     /gene="gap"
                     /locus_tag="PKY63_12290"
     CDS             1892..2902
                     /gene="gap"
                     /locus_tag="PKY63_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPF01438.1"
                     /translation="MKKKIAINGFGRIGRLTFQALLKNNDVDVVAINDLTNAKTLAHL
                     LKYDSVHGRFEGSVEADDNSITVNGEKYIIYAEKEPANLPWKDLGVDIVVECTGRFRK
                     RADLEKHLTAGARRVALSVPADKSDDVDLTVVIGVNDNLLNKDHVLVSNASCTTNCLA
                     PVAKVLNDTFGIEHGLMNTIHSYTNDQIILDAPHKDLRRARAAAVSIIPTSTGAAKAI
                     GLVIPELTGKLDGMSMRVPTPDGSVVDLTVVLKKTVTAAEINSAMKQAADGPMKGVLE
                     YTSDPIVSADIVDNSHSSIFDSLLTQVISGNMVKIVSWYDNEFGYSQRLADLAVKLSK
                     LD"
     gene            2905..3093
                     /locus_tag="PKY63_12295"
     CDS             2905..3093
                     /locus_tag="PKY63_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01439.1"
                     /translation="MSLLIVIGICIALVGLAFAGIAIKMFLKKGGEFKKQCSVKDPQT
                     GKSLGCSCGGHDKTCHNS"
     gene            3119..3751
                     /gene="folE"
                     /locus_tag="PKY63_12300"
     CDS             3119..3751
                     /gene="folE"
                     /locus_tag="PKY63_12300"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPF01440.1"
                     /translation="MKKEKRIDNYPDDNYYRIDHYDEENLNKLKVHYNEVLKLIGEDP
                     QREGLIKTPERVAKSMQFLTHGYHANPEQILRSAMFREDYREMVIVKDIEVYSLCEHH
                     MLPFFGKAHVAYIPNGYIVGLSKIPRIVDAFSRRLQIQERLTTQIKDCLQDVLQPLGV
                     AVVIEAHHLCMMMRGIQKQNSITTTSDFTGVFKNDKTRAEFMTLISAQLH"
     gene            3773..>4332
                     /locus_tag="PKY63_12305"
     CDS             3773..>4332
                     /locus_tag="PKY63_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPF01441.1"
                     /translation="MEHNTNYSDPQVFTKEEAGVRNSFVAKVFSWMFAALLITAVVSF
                     LFGSQESLIRLLINESGMTILGWIVMLAPLGLVILMSFGYNKMSATTMSIVFILYSAL
                     MGASLGFIFIAYQLGTIGLAFIVTSGMFAIMAIAGYTTKMDLTKFGNILMIALVAIII
                     GSVVNMFMRNDTMDFIITIVGVLVFTG"
BASE COUNT         1246 a         1018 c          864 g         1204 t
ORIGIN      
        1 tcggaagctt cgccatggaa gccggctgca atgatgtgac gacctgtttt tcattatcca
       61 gcacccctgc aacaaatttt acagtcaact ataccattct gggcaccgca accaatggtg
      121 ttgattatcc caccattcct tcttcactca caattccagc aggatccgac tcggtttgta
      181 ttaccatcag tccaaccatg gacaatctgg ctgaaggcgt agaaacaatc atgctggtat
      241 tacaaaacca aatcagctgc aacaccgttt ccgatacagt tgaaatcatg attcttgatt
      301 acgaacacgt ttcgacactc atgtcaccag acaccagtat atgcggagac actgttaata
      361 tctgggcttt aggcacactt ggcgttcccc catataatta tgcatggaat aacggactgc
      421 catcaaccac cagtcatctg gtttcaccaa ccactaccac tacctatgtt gttactgtta
      481 ccgattactg caatcatagt acaacaaaca atgttaccat tactgtcggc ggctcctttg
      541 tcgatgcagg ccccgaccaa accatctgcg aagggcaaag cgccacactc aatgccgcct
      601 ctgacaattc cattgtttgg aacaccggcg acacaacact gagtattaca gtttctcccc
      661 ctcatgactc cacctatgtt attatagccg gttcaggtga ttgtatgagc agcgataccg
      721 ttactgtttt tgtattgcct caaccagaca tttccgcatc ggtttttccc gacaggatat
      781 gcgaaggaaa atcggctcag ctgacagcaa ctggcgccga catgttcctt tgggaaagca
      841 atccttttga tgcaaccctc aatcaaacac ccggcggact gaataatatg ctggatatta
      901 ctccatcatc aacaaccaca tacaatgtta ccggaaccaa tgttggcggc tgttttgata
      961 ctgttacggt gaaaatcaca gttgttcctg ctccagtagc atcattcttt acacaaccac
     1021 cctatgcttc atcattcaat ccaaccatac agtttttcga caattcacag ggcaaccccg
     1081 ttctctggcg ttggcttctg agtgatggct caacttacga cattcctgaa ttcacccata
     1141 catatcccat ggacacctgg ggagaatttc ctgttttaat gtatgttgaa aatgctgatg
     1201 gatgcagtga ttcaataaca caaatgatta ccatcaaacc agattacacc ctgtatattc
     1261 ccaacgccat ttcacccaac ggagacggca ttaacgaccg ttttcacatc agcggactta
     1321 atattcccat taaggatttt tcaatacgta tttatgaccg ctggggtgct ctgatgtttt
     1381 cttcctccgt tccttctttc gaatgggatg gctatgttga cggcaaagtt gttcctgatg
     1441 ctatttacaa ttacagactc attttcagcg acccaaccgg gaatgtacac attagaaacg
     1501 gcagcatcac gattatttac tgaaaaacac agtcaaactg attggtattc ttctattcgg
     1561 aaatgccctt taatgttttg aatcaagtga tttttgtgag gtaaatctct caaattcaat
     1621 aatcatgtaa cttgtgtcat tatcaggttc aaaatatcac actaattcat tttcactcaa
     1681 ctcgcacagc tgagacaaac aagttatcct ggcggcacca tcggagagtc gcctgaaatg
     1741 cagagtcagt aagggcttca cgtaaaatac aacaaagtgg ttgctgtagc aatatcacgg
     1801 taccgaagca tgttctcata tccaaaaaac aacagaacag gttttgcgaa tgcagttatt
     1861 ttgtactttt gcactgataa aaacaattgc tatgaaaaag aaaattgcca taaatggttt
     1921 tggcagaatc gggcgcctta cttttcaggc cctgcttaaa aacaacgatg ttgacgtggt
     1981 tgccattaat gacctcacca atgcaaaaac gctcgctcac ctgctcaaat acgattctgt
     2041 tcacggacgt ttcgaaggaa gtgttgaagc tgatgacaac agcattaccg ttaatgggga
     2101 aaaatatatt atttatgctg aaaaagaacc ggccaatctt ccctggaaag accttggtgt
     2161 tgatatcgtg gttgaatgca ctggtcgttt ccgtaaaaga gccgatcttg agaaacacct
     2221 gacagcaggt gcccgacgcg tagctctgag cgttcctgct gacaaaagcg acgatgtgga
     2281 tctgactgtt gttatcggcg taaacgacaa cctgctgaac aaggatcatg ttcttgtgag
     2341 caatgcaagt tgtactacca attgcctggc gcctgtggca aaagtgctga acgacacttt
     2401 tggaattgag catggcctga tgaacaccat tcactcatac accaacgacc agattattct
     2461 cgatgcgcca cataaagatc tgcgccgtgc gcgggctgca gctgtatcca ttataccaac
     2521 gagcaccgga gctgccaaag ccatcggcct ggtaattcct gagcttaccg gcaaactcga
     2581 cggcatgtcg atgcgcgttc cgacacccga tggatccgtt gttgatctca ctgttgtttt
     2641 gaaaaaaacc gttactgctg ctgaaatcaa cagcgccatg aaacaagcag cagacggccc
     2701 catgaaagga gttctcgaat atacatcgga tcccattgta agtgccgata ttgttgacaa
     2761 ctcacattct tccatttttg attcactgct cacacaggtt atcagtggaa atatggtaaa
     2821 aattgtttca tggtacgaca acgaattcgg ctattcgcaa cgtttggccg atctggctgt
     2881 taaactcagt aaactcgatt aattatgtca ttgttgattg tcattggaat ttgtattgct
     2941 ctggttggac tggcctttgc cggtattgcc atcaaaatgt ttctgaaaaa aggcggcgaa
     3001 ttcaaaaagc aatgctccgt gaaagaccct caaacgggta aatcgctcgg atgcagctgt
     3061 ggcggccatg ataaaacatg tcataattcc taatgaatat cacgttataa aaaccaaaat
     3121 gaaaaaagaa aagcggatag ataattaccc cgatgacaat tactatcgca ttgatcatta
     3181 cgacgaagaa aatctgaaca aactcaaagt tcattacaac gaagtgctga aactcattgg
     3241 cgaagatcca caacgtgaag gccttataaa aactccggaa cgcgtggcca aatccatgca
     3301 atttctcaca cacggctatc atgcaaaccc tgaacagata ctgcgttcag ccatgtttcg
     3361 cgaagattac cgcgaaatgg tcattgtcaa agatattgaa gtgtattccc tttgtgaaca
     3421 tcatatgctg ccgttctttg gaaaagcaca tgtggcctat attcccaatg gatatattgt
     3481 tggactgagt aaaattccac gtattgtcga tgctttttca agacggctcc aaattcaaga
     3541 aagacttaca acccagataa aagattgtct gcaggatgtt ttacaacccc tgggtgttgc
     3601 cgttgtcatc gaagctcatc atttatgcat gatgatgcgt ggcattcaaa aacaaaacag
     3661 cattacgact acttcagatt ttacaggtgt tttcaaaaat gataaaaccc gtgcagaatt
     3721 catgactctc atcagcgcac aacttcatta agcaacaaaa aaaacaacaa atatggaaca
     3781 taacaccaat tattccgatc cgcaggtatt taccaaggaa gaggcaggcg tacgcaacag
     3841 ttttgtcgca aaagtgtttt cctggatgtt tgccgcactt ctcattactg ccgtagtttc
     3901 atttcttttc ggctcacagg aatctttaat ccgtttgctg atcaatgaat ccggaatgac
     3961 catccttgga tggattgtca tgcttgctcc tctcggactt gttatactga tgtcatttgg
     4021 ctacaacaaa atgtctgcaa caaccatgtc cattgttttt attctgtatt cagcattgat
     4081 gggagccagt ctgggattca ttttcattgc atatcaactt ggcaccatcg gacttgcttt
     4141 tattgttacc tcaggcatgt ttgccattat ggccattgcg ggttacacaa caaagatgga
     4201 tctgacgaaa ttcggaaaca ttctgatgat tgcgctggta gccattatca taggaagcgt
     4261 ggttaacatg ttcatgcgga acgatactat ggattttatc atcaccattg tgggcgtgct
     4321 tgttttcacc gg
//
LOCUS       DAOPKE010000151         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22589_length_4279_cov_15.276515, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000151 DAOPKE010000000
VERSION     DAOPKE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22589_length_4279_cov_15.276515"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..546)
                     /locus_tag="PKY63_12310"
     CDS             complement(121..546)
                     /locus_tag="PKY63_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01442.1"
                     /translation="MKSKLYNLPDCKCKNAHPVLLRVIVNKSFTRLDFGYQATSLYTR
                     GGWVTIAPDTYLRTSERSPGLKMIRAENIPVGPEKHFFESTTDRMYFSLIFEPLPAGT
                     TKFDLIEKLPDTPTLFNFFGIKLKAEDAIELLEEQLNNE"
     gene            complement(536..1561)
                     /locus_tag="PKY63_12315"
     CDS             complement(536..1561)
                     /locus_tag="PKY63_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF01443.1"
                     /translation="MRNKEAYSRYKIIDSCLSNRFRPYPTMQDLLETCEDKLDKRFSV
                     STIQKDIQAMKFDQSLGFNAPIEFSRKHNGYYYSEQNYSISGVPLTEEELSNLKSVAD
                     MLSSFTGKRISETYDQAVNKIMTSLREEDLNQRNRPKLIETDPHNTQRGFENFEKLFY
                     AVEHRIPISFVHYSYDKRRFNAVIAHPVLLKEFQSRWYLMAWSEQHKGLMTFGFDRIH
                     EPLLLKRKYIKTDQEEIDRHTKSIYGVFPFEGESVQKIRFRTDSLFTDYLMASPLHPS
                     QQLLEMYEHGSALFSVELIPSMELLNFFLEHSTRIHLVGPATMRKRMLKALQEAVNRY
                     KMFEYEK"
     gene            1987..2736
                     /locus_tag="PKY63_12320"
     CDS             1987..2736
                     /locus_tag="PKY63_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01444.1"
                     /translation="MMKIKTYKPLKPEETVTFRRINFFGVTRKFNKEALALHPEMLPV
                     LRISDLTGCEHGFIMIQLSYSDLFAQATAQSYPEYEETYYTVFKNGELDSKNAIPLYP
                     ETEGKHLISISIGLCSRTEEATLTLTVNCEEESDLKQVMKKQSDLPGSTIKFHVMQDW
                     SDDLSWCPSQCIKKIEYQGRHFILLLYWRHQDPWTAEITECHPDGTFTPLDDETATYP
                     LNIGYWKDSELRFAKNSAEVGMVEWLREECC"
     gene            2879..3397
                     /locus_tag="PKY63_12325"
     CDS             2879..3397
                     /locus_tag="PKY63_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01445.1"
                     /translation="MKSATIQMKPQDVVILLKIIAIRSDNWQQKPLADSLKMSQSEIS
                     QAASRMQYAGILLGKKVMRLAFFDFLKYGLSYVFPQKPGPIVRGMPTAHSALPLNKKI
                     ISEEQFVWPYAAGKVRGHGIVPLYSSVPDAAMNDRALYELLALVDAVRVGRTREKDLA
                     LKELEKRIVNGK"
     gene            3387..4073
                     /locus_tag="PKY63_12330"
     CDS             3387..4073
                     /locus_tag="PKY63_12330"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPF01446.1"
                     /translation="MENKTINLGMVSDVAKALQELNEKMVFVGGAVISVYTDDPAADE
                     IRPTSDIDMTIKLATYSEWVNLEKRLLELKFAPNPEGHALCSFLYNKIEIDIMPSEPG
                     ALGDSNRWYEPGFESLQEIKIHGQTIRVLSAPYFIATKLEAFHSRGNDYRTSHDFEDV
                     IYIIDNRTTIVNEVADADDAVKQYLKDEFTKILSNKNFPEIISAHIHPLMLEERMSIV
                     IDKLKNMCNY"
BASE COUNT         1242 a          977 c          911 g         1149 t
ORIGIN      
        1 gagatcccgg gtcacatgaa ctctgaacag cgaacctgtc tgcaacaggc aggcaaggaa
       61 gtgagaatta cgaaggaaat ctacttcaaa cttacttctt ccttcatatt tccttgttct
      121 ttattcatta ttcaactgtt cttccagcaa ttcaatcgca tcctccgctt tcagtttgat
      181 accaaagaaa ttgaataaag tcggggtgtc cggcaatttt tcaatcagat caaactttgt
      241 tgtacctgcg ggcagcggct caaatatcaa actgaaatac atccggtctg ttgtactttc
      301 aaagaagtgc ttctccggac caaccggaat gttttcggct cgtatcattt tcaatccagg
      361 acttctttcg cttgttctta aataagtgtc cggtgcaatg gtcacccatc cgccgcgggt
      421 gtataaacta gttgcctgat atccgaaatc aagccgggtg aagctcttgt tcacaataac
      481 ccgcaacaaa actgggtgtg cgtttttgca tttgcaatcg ggcaaattat ataatttact
      541 tttcatactc aaacatttta taacggttta cagcttcctg caacgctttg agcattcgtt
      601 tgcgcatggt ggccgggcca accaaatgta tgcgcgtgct gtgttcaaga aagaaattca
      661 gcaactccat gctgggaatc agctctacac taaacaaagc cgatccatgc tcatacatct
      721 ccagtaactg ttgcgacgga tgcagcgggc ttgccatcag ataatcggta aacagcgaat
      781 cggtacggaa acgtattttc tgaaccgatt ctccttcaaa cggaaacact ccgtaaatac
      841 tttttgtgtg ccggtcaatc tcctcctgat ctgttttaat gtatttgcgc ttcagcagca
      901 agggctcatg aatacggtca aagccgaatg tcatcagacc tttgtgttgc tccgaccagg
      961 ccatcagata ccacctcgat tgaaattcct tcagcaggac cggatgtgca atcaccgcat
     1021 tgaatcggcg tttgtcatag gaataatgaa caaaactgat tggaatccgg tgctcaacag
     1081 cataaaatag tttttcgaaa ttttcaaagc cgcgctgggt attgtgcgga tcggtttcaa
     1141 tcagtttcgg gcggttccgc tgattcagat cttcctctcg caacgaagtc atgattttgt
     1201 tcacagcttg atcataagtc tcgctgattc gtttgcccgt aaacgacgaa agcatatcgg
     1261 ccaccgattt cagattgctc agttcttctt cggtaagtgg aacgccgctg atgctgtagt
     1321 tttgctccga atagtaatac ccgttgtgct tgcggctgaa ctcaatcggc gcattgaacc
     1381 caagcgactg atcaaacttc atcgcctgaa tgtctttctg aatggtggaa actgaaaacc
     1441 gcttgtcgag tttgtcctca caggtttcca gtaaatcctg catggtcgga tagggccgga
     1501 aacgattgct taggcaactg tcaatgattt tataccggct gtaggcctct ttgtttctca
     1561 tggaatcaga tatttcaata cttgcgaaaa cgataaatta ctgcaattta caataaaaaa
     1621 ccaaaccgca aacacattgc gttttgatca tggattggcc acaagggcac gaagacacaa
     1681 aatattttta cactaccgag ttcccggtca cagattgcga tacagcttct gccaccgtgc
     1741 cgggatctcc tcgaaaacct tcgaagccct tgctgaatat acatttcagg agaccccgga
     1801 tctggcgtcc ggggacttgg atggtgttag acgaacggac aaatgcgaat cccctttcgc
     1861 ctgcggcaaa agctgattca gagggtgcat gttccgtaac tcataactta tcactcataa
     1921 ctcataactg aaaatgcaaa ccgcaaactc cctgcgtttt gccttcgcat atttgcagaa
     1981 aagcacatga tgaaaattaa aacatacaag ccgctgaaac ccgaagagac tgtcaccttt
     2041 cgccggataa atttctttgg cgttacccgc aaattcaata aagaggcgct tgccttacat
     2101 cctgaaatgt tgccagtgct tcgaatttca gacctgaccg gttgcgaaca tggcttcata
     2161 atgatacagc tctcctattc agatcttttc gcgcaagcta ctgcacagag ttatccggaa
     2221 tatgaagaaa catattacac cgtattcaaa aatggtgagc ttgattcaaa aaatgcaatt
     2281 cctttatatc cggaaacaga aggcaaacac cttatttcaa tctccatcgg cctttgctcc
     2341 cggactgaag aagcaacact aacgctaact gtaaactgcg aagaggaaag tgatctaaag
     2401 caagtgatga agaaacaaag tgacctgcct ggttcaacca ttaaattcca tgtcatgcag
     2461 gactggagcg acgatctctc ctggtgccct tctcaatgca ttaagaaaat tgaataccag
     2521 ggccggcatt ttatcctgct cctctactgg cgccaccagg atccctggac cgccgaaatt
     2581 accgaatgcc accccgacgg aacattcacc ccactagatg atgaaaccgc cacatacccg
     2641 cttaatatcg gatattggaa agactctgaa ctgcgctttg caaagaattc tgcggaagtg
     2701 gggatggtgg agtggttgag ggaggaatgc tgttaaagaa gagtcgtttt ggagtggagc
     2761 ctatagctct gtatgaaata tcgcatatcg cgatatgcga taaattattt aaaaacattt
     2821 gtttatcaat gatttttgtt gtaatattgc atatcgcaat atgcaatatt taaaaaacat
     2881 gaaatcagca actatacaaa tgaagccgca ggatgttgtc attctgctaa agattatcgc
     2941 tatacgcagc gacaattggc agcaaaaacc attggccgat tcattaaaga tgagtcagtc
     3001 ggaaataagc caggcagcat cgcgtatgca atatgccggg atactgttgg gcaaaaaggt
     3061 gatgcggctt gcttttttcg attttctgaa atatgggctc agctatgtgt ttccgcaaaa
     3121 gcccggaccc attgtaaggg gcatgcctac tgcgcactcg gcactaccgc tgaataaaaa
     3181 aattataagc gaagaacaat ttgtatggcc ttatgcggct ggaaaagtaa gaggacacgg
     3241 cattgttcca ctctattcgt cggtgcccga tgcagctatg aatgatagag cactgtatga
     3301 gctgctggcg ttggtcgatg cggtcagagt aggccgcaca cgcgaaaaag atctggcgct
     3361 gaaagaactc gaaaaaagaa ttgtgaatgg aaaataaaac aatcaatctc gggatggtgt
     3421 ccgatgtcgc caaagccttg caggaactga atgagaaaat ggtttttgtg ggtggcgctg
     3481 taataagtgt ttacaccgac gaccctgctg ccgacgagat tcgtcccaca tccgatatag
     3541 atatgaccat taagctggca acctattccg agtgggttaa tcttgaaaaa aggttgctgg
     3601 aactgaagtt tgcacctaat ccggaaggac atgcgctttg cagtttttta tacaacaaga
     3661 tagaaattga catcatgcca tcggagccag gtgcattggg cgattccaat cgctggtatg
     3721 aaccgggctt tgagtcattg caggaaatta aaatccatgg tcaaaccatt cgtgtgctct
     3781 cagcacccta ttttattgcc accaaacttg aagcttttca cagcagaggc aatgattaca
     3841 ggaccagtca cgattttgaa gatgtaatct atatcattga caaccgcacc actattgtta
     3901 atgaagtggc agatgccgat gatgcagtta aacaatatct caaagacgaa ttcacaaaaa
     3961 ttctgagcaa taaaaatttc cccgagatta ttagtgctca tattcatccg ttgatgctgg
     4021 aagagcggat gtcaattgtg atagataaat taaaaaatat gtgcaattat taatatcaat
     4081 agcccaaatt cagtagaaca ttttatcatt taccaactcg ccgcagcgga ccgaatgcca
     4141 ccccgatgac acattcactc cactgaccac agacaattca caactgacta cttttacgtc
     4201 gcctttcaac tttaaaaact ttataaactt tccactttac tatcggacat tggaaagact
     4261 ccgaattgcg ctttgcaaa
//
LOCUS       DAOPKE010000152         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22635_length_4273_cov_16.059507, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000152 DAOPKE010000000
VERSION     DAOPKE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22635_length_4273_cov_16.059507"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1406)
                     /locus_tag="PKY63_12335"
     CDS             complement(42..1406)
                     /locus_tag="PKY63_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPF01447.1"
                     /translation="MNSMNKLLFSFLLFFFAGFYINTQAQDKPDMPVKIYVLKIFDEI
                     GPSSWRTTQNGLSEARELNADIVIVHLNTYGGLVDAADSIRTALLNFPKPVWVFIDNN
                     AASAGALISIACDSIYMRKGANIGAATVVTETSEAAPDKYQSYMCSMMRSTAQAQGRD
                     PMIAEAMVDPDVYIEGIVDSGKVLTFTAEEAMANGYCEAQAENIEEIIERNGIDEYDI
                     VRQEISAMDRFIGFLINPAISGLLIMLIIGGIYFEMQSPGIGFALLVAVLAALLYFAP
                     LYIEGLANNWEIIIFVIGIGLLAVEIFVIPGFGVAGVSGIILIVGGLTLSLVGNIGLS
                     MPDGDYAPVARSFALVSFSILLALVGSFYLSSKLVKVKVGGSTLGLSDELRSADGYSA
                     SESTYNLLIGKNGLALTILRPSGKIEVDGEQYDATAQIGYIEKGEKVRIVAYENMQFV
                     VRKV"
     gene            complement(1390..2142)
                     /locus_tag="PKY63_12340"
     CDS             complement(1390..2142)
                     /locus_tag="PKY63_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01448.1"
                     /translation="MKHIILLLFVFFCLFSSLLAQNPYVEQSFPWLNLQVVKDQKIKS
                     IMLATVSAENASVVNQVLLSFDPSGRLVKEETQSGFRHSCEMYYDHHGLLKKVISQYG
                     TSQDSVVYSYDKQNNAFVSQFLSREESGTWSVTETRYVYSGENKLLEIQTSEGSVKVL
                     QPAGKNNLALVSTEKITHTGFEIFSSVGDISKTYVYDASGHLLSYSFTNALYGMTEES
                     VYSYDQHDRLVMITWSQEGLTSKTFVTYEFYE"
     gene            complement(2161..2522)
                     /gene="rnpB"
                     /locus_tag="PKY63_12345"
     ncRNA           complement(2161..2522)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKY63_12345"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2680..3837
                     /locus_tag="PKY63_12350"
     CDS             2680..3837
                     /locus_tag="PKY63_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01449.1"
                     /translation="MKKILLLLLVAAFAASCTSSKKYLERQQFDYAIQKSSKKLMKNP
                     DKEKQLDVLNRAWKAANQRDMERINYLKTTGQPEIWDEIFQIYNRMKWRQDIVHNLPQ
                     PVLSSIGYVYVDYTNETVNAQRNAAEFYYQKAMTLMESGDRFSARQAYDHLLQTKRFF
                     AAYKDVDQQILRAQAMGTANVLFISRNNSGQMLPEGFLDEILKVSMGDIEGRFVRFFN
                     TFDTSRQYHYRTVLNLTKVDVSPESAREFFYEESKEVQDGWTYVLDSNGNVMKDSLGN
                     DIKIPKMIEISCQVHETQLHKQAIVGGYLDIYGADNTLIKSDPITAEVFFDYAFATAN
                     GDKSILKEETKRKLSNPPTGFPNTFQMIWDASSIVKNIAKDILVRNSYIFQ"
BASE COUNT         1372 a          982 c          804 g         1115 t
ORIGIN      
        1 tttaatcagg aaatacaaca caataatcaa aagaagataa cttatacctt tctaacaaca
       61 aactgcatgt tttcgtaggc aacaattcga actttttcgc ctttttcaat ataaccaatc
      121 tgagcagtgg catcatattg ttcgccatcg acttcaattt ttccggaagg tctgagaatg
      181 gtcaatgcca gcccattttt accaatcagt aagttataag tactttcgga agcagaataa
      241 ccatccgccg agcgcaattc gtcgcttaag ccaagagtag aacctccaac tttaacttta
      301 accagctttg aactaagata aaaagagcca acaagtgcca acagaataga aaatgaaacc
      361 aaggcaaagg agcgggcaac gggagcataa tcaccatcgg gcatactcag gccaatattt
      421 cctacaagcg aaagtgtaag acctccaaca ataagaataa ttcctgaaac gccggccact
      481 ccaaaaccgg gaataacaaa tatttccaca gccagaagcc ctataccaat gacaaaaatg
      541 atgatttccc agttattggc cagcccctca atataaaggg gtgcaaaata aagcagggcg
      601 gcaaggacag ccaccaaaag agcaaatcca attccgggag actgcatttc gaaataaatg
      661 ccaccaatta tcaacataat cagcaatccg ctgatagccg ggttgattaa aaaaccaata
      721 aatctgtcca tagccgaaat ttcctgacga acaatgtcat attcatcaat tccgtttctt
      781 tcaattattt cctcaatatt ttcggcctga gcctcgcaat aaccattagc catggcttct
      841 tcggcggtaa aggtcagtac ttttcctgaa tcaacaatac cttcaatata aacatccgga
      901 tcgaccattg cctccgctat catcggatca cgaccctgcg cctgggcagt gctgcgcatc
      961 attgaacaca tatagctctg gtatttatct ggcgccgcct cagatgtttc tgtaacaact
     1021 gtagccgctc caatattggc tcctttgcgc atataaatgc tgtcgcaggc aatggagatc
     1081 aaagctccag ccgaagcagc attgttatca ataaaaaccc agaccggctt tggaaaattt
     1141 aacaaagctg tgcgtatgct atcggcggca tcgaccaaac caccataagt attcaaatgc
     1201 acaataacaa tatcggcatt cagttcccgg gcttcgctca atccattttg tgttgtccgc
     1261 catgaggatg ggcctatttc atcaaatatc ttcagaacat aaattttcac cggcatatcc
     1321 ggcttgtctt gcgcctgagt attgatataa aaccctgcga aaaaaaagag cagaaacgaa
     1381 aacaatagtt tattcataga attcataagt gacaaaagtt ttactggtca gaccttcctg
     1441 agaccaggta atcatgacca aacggtcatg ctgatcgtag ctgtaaacag attcttcagt
     1501 cattccatac agcgcatttg taaaggaata agagagtaaa tgaccagaag catcataaac
     1561 ataagttttg gaaatatcgc caacggaaga aaagatttca aaacctgtgt gagtgatttt
     1621 ttcagttgaa accaaagcaa ggttgttttt gccggcaggc tgcaatactt tcaccgaacc
     1681 ctcagatgtt tgaatttcta aaagtttatt ttccccggaa taaacataac gggtttcagt
     1741 aaccgaccag gtacctgact cttcacggct gagaaattga gacacaaaag cattattttg
     1801 tttgtcgtat gagtaaacaa cggaatcctg cgaagtccca tattgactaa taaccttctt
     1861 taataatcca tgatgatcat aatacatttc acacgaatgc ctgaacccgg actgagtttc
     1921 ttcttttacc aaacgccccg atggatcaaa agacaaaagg acctgattga caaccgaagc
     1981 attttcggcg ctgactgttg ccaacataat acttttaatt ttctgatctt tcacaacctg
     2041 cagattgagc catggaaaac tttgctcaac ataaggattc tgagccaaca gcgaggaaaa
     2101 aagacagaaa aaaacaaaca aaagaagaat gatatgtttc atgcgaatca gatttcaaaa
     2161 aaaacaagct gtcccataag ccggattctg taatccaccg aagcggactc tcatcattta
     2221 tctgcggtac atgtcgccat gaacctttag cagcctaccc cccggcatgg gcgagcaacc
     2281 ctgagccggt atatgtggcc ttgcacccca tgaggtttac ctgctgcgga gtcgcccccg
     2341 acagctgtga gctcttgcct cacaatttca cccttaccgc agcataaagc tgtggcggta
     2401 tcatttctgt tgcactggct gtcccgccga aacgggcctt cccgttagga agcatggtgc
     2461 tctgtggtgt ccggactttc ctccctttaa aaaaatcaaa gagcgatgag agaaacagct
     2521 tgatacaaaa atacaccgtt ttttcgagaa aaatacaatt gacattcatg aatttagcag
     2581 ttatatgcaa attatcttta tatctgtggc gcatttttcg tattattgtg gcaaaataat
     2641 tgaaaggcca ttggccagaa cccactaaaa cctgcagcca tgaaaaagat acttttacta
     2701 ctgttagttg ctgcatttgc tgcatcctgc acaagctcga aaaaatacct cgaacggcag
     2761 caatttgatt atgcgataca gaaaagttcc aaaaaactca tgaaaaaccc ggacaaagag
     2821 aaacaactcg atgttcttaa ccgggcgtgg aaagccgcca accagcgcga catggaacgc
     2881 atcaattacc ttaaaaccac cggacaacct gaaatctggg acgaaatatt ccaaatctat
     2941 aaccgcatga aatggcgtca ggacatcgtg cacaatctcc cacaacccgt cttgtcttcc
     3001 atcggttatg tatatgttga ttataccaat gaaaccgtaa atgcccaaag gaatgccgcc
     3061 gaattttatt atcagaaagc catgacgctg atggaaagcg gcgaccgttt ttcagcacga
     3121 caggcatacg atcatctgtt acagaccaaa cgtttctttg ccgcatacaa agatgttgac
     3181 cagcagattc ttcgtgctca ggccatggga accgccaatg ttctgttcat ttcgcgcaac
     3241 aattccgggc agatgctccc cgaaggtttt cttgatgaaa ttctcaaagt ttcaatgggc
     3301 gatatcgaag gccggtttgt tcgcttcttc aacacctttg atacttcacg tcagtatcat
     3361 tacagaacag tattgaacct gactaaagtt gatgtatcgc ccgaaagtgc ccgtgaattt
     3421 ttctacgaag agtcgaagga agtgcaggat ggttggactt atgtgctcga cagcaatgga
     3481 aatgttatga aagacagtct gggcaatgac atcaaaatac cgaaaatgat tgaaatttca
     3541 tgtcaggtac atgaaacgca actacacaaa caagccattg ttggtggata tcttgacatt
     3601 tacggagctg acaatacact cataaaatct gatcccatca ccgcagaagt tttctttgat
     3661 tatgcttttg caacagccaa cggagacaaa tcaattctca aagaagaaac caaacgcaaa
     3721 ctcagcaatc ctccaactgg ttttcccaac actttccaaa tgatctggga tgcttcgtct
     3781 attgtaaaaa acattgcaaa agacatattg gtgcgaaatt cctatatttt tcaatagtta
     3841 ttcttttcaa aaaaaagctg tcgtaacccg acagcttttt ttattttaat catcacccgt
     3901 aaaacataat caacatagta tttccaaaac ggcctggcaa cgacaacctc agcgaagtcg
     3961 catattcagg cattatcaga ggacgcagct ctttatatgc gactcctccg gagtcgttgt
     4021 tttgtgtatt tagggtctgc tgaaaaacac gtaacactca caaaaacaga aatatattca
     4081 tcaaattcag caatgcaaat gcaagtgttt tcatcaggtc aatgaaataa ccttgccctt
     4141 gagaaactgt tgatgaaatt tttgaagttg aaaaaacatt attgaatggt ggctcatcag
     4201 ttcaataaaa ccacaaatat tgctgtgagt gtttgcttat gccgtttcaa tgcttatttc
     4261 atatgcacgg tat
//
LOCUS       DAOPKE010000153         4270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22667_length_4270_cov_14.829181, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000153 DAOPKE010000000
VERSION     DAOPKE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22667_length_4270_cov_14.829181"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3323)
                     /gene="mfd"
                     /locus_tag="PKY63_12355"
     CDS             complement(<1..3323)
                     /gene="mfd"
                     /locus_tag="PKY63_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPF01450.1"
                     /translation="MKLSEAIHPLVAPVSETGCFKLLAEKISGGHSRVISLEGLAGSA
                     APLLCAFNSVVQNRSQIIIAANREDAMSFYDDMENLLSQKDMPPGDKSVAFFPTLYRK
                     EHAPAETDSRHLLMRAEALRSLHGNKPTCLITWPAALCTRVPNAGFMQQNSLTLNAGE
                     KIDLDFIEEVLTGYDFRQVDFVCEPGEFALRGGICDVFSFADENPFRITFSGNTAESI
                     RSFDPASQLTISNHDTIQILPDLSRMQNDHDLVPVFDLFPEDTSIWINDPSAAQRFRE
                     ECVRLENSGAIPAEKILDADGWSGSIEKFIQVYTCPTPLSVNDSDRIVFSFSPQPPVN
                     RKFDWFVEEVFSRLSNGWSISIIAENAQQIERLYAILKDIESQKRISITDKISILKFS
                     LGEGFSDNLSKVAIYTDHQLFNRYRRSSLHDRFSRPERLSINELVNLQPGDYVVHINH
                     GIGRYAGLEKVEVNGQIQEAIRLLYKDDDELMVSIHALHRISKYIGKDGSPPSIDRIG
                     SASWTTRKNKAKKKVKDIARELIKLYAKRRAAKGYAFSPDTYLQHELEASFIYEDTPD
                     QATATEAIKADLEADFPMDRLICGDVGFGKTELAVRAAFKAVADSKQVALLVPTTILA
                     YQHYCTFKERLDELPARVEYMNRFRSTKEQKETLKKLKEGQVDILIGTHRILSKDVEF
                     KNLGLLIVDEEQKFGVGAKEKLRNLKENVDTLTLTATPIPRTLQFSLMGARDMSVLRT
                     PPPNRYPVQTEVMTFDPEVVRDAVRFEIQRGGQVFFVHNRIQNIQDIANLVRSLVPEA
                     RVDIGHGQMPGDEMEKVMLRFVAGEIDVFISTTIVESGLDVPNANTMILNDAQNFGLS
                     DMHQLRGRVGRSNKKAFCYLFTPPEVILSDESRKRLRAIAEFSDLGSGFQIAMRDLDI
                     RGAGNILGAEQSGFISEIGYEMYQKILDEAIAELKDDEMPDAAGKDQRAPRRYVHDVV
                     IETDASILIPDKYVPSVSERLLLYKELNEIENEESLSTFRSMLEDRFGAIPKETAELI
                     SAVRLKWLAKNMAIEKLILKKGLLTLYFISRKESPFFNSATFQYIIDFTVKNPRRCLM
                     KEHNERLIMKVDKIQSVGAA"
     gene            complement(3733..3987)
                     /locus_tag="PKY63_12360"
     CDS             complement(3733..3987)
                     /locus_tag="PKY63_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPF01451.1"
                     /translation="MNIYVGNLNYKVNGDRLKSVFSEFGEVESAVVITDKFTGKSKGF
                     GFVTMTNDDDAQRAIAELNGKDVDGRQMVVNQARPREENR"
BASE COUNT         1173 a         1026 c          897 g         1174 t
ORIGIN      
        1 gccgccccca ccgattgtat cttatccact ttcattatca ggcgctcgtt gtgctctttc
       61 attaaacagc gacgcggatt tttcaccgtg aaatcaataa tgtactgaaa tgtggcggaa
      121 ttgaaaaatg gtgattcctt gcgcgaaatg aaatataaag tgagcaatcc ttttttcaga
      181 atcagttttt cgatcgccat gttttttgcc agccacttca aacggaccgc cgaaatgagt
      241 tcagccgttt ctttgggaat ggctccaaat ctgtcttcga gcatgcttcg aaaagttgac
      301 agagattcct cgttttcaat ttcattgagt tccttataca gtaaaagccg ctcagaaacg
      361 gaaggaacat atttatccgg aatgagaata cttgcatcgg tttcaatcac cacatcatgc
      421 acatatcttc gaggagcgcg ctggtccttg cctgcagcat cgggcatctc atcgtctttt
      481 aattcggcaa tggcttcatc aagaattttc tggtacattt catagccaat ctctgaaata
      541 aaaccgcttt gctctgcacc cagaatattg ccggcgccgc gaatatcaag gtcgcgcatc
      601 gcaatctgga agccggatcc aagatccgaa aattccgcga tagctcgtag gcgtttgcgc
      661 gattcatcac tcagaatcac ttcgggcggc gtgaataaat aacagaatgc ttttttattt
      721 gaacggccca cgcgtccgcg taactggtgc atgtcgctca gaccgaagtt ctgtgcatcg
      781 ttcagaatca tggtgtttgc attcggtaca tcaagaccgg attccacgat ggtagtggaa
      841 atgaaaacat ctatttcacc ggctacaaaa cgcagcatca ctttttccat ctcgtcgccg
      901 ggcatctgtc cgtgaccaat atccacgcgg gcttcgggaa ccaaggagcg aacaaggttt
      961 gcaatatcct gaatattctg aatccggttg tgaacaaaaa acacctgccc tccgcgctga
     1021 atctcaaacc gaacagcatc gcgaacaact tccggatcaa aggtcatcac ctctgtctgc
     1081 accggatagc ggttgggtgg cggagttcgc aacacactca tgtcgcgcgc acccatgagc
     1141 gaaaactgca gggtgcgcgg aatcggcgtc gcagtcagcg ttagcgtatc gacattttct
     1201 ttcaggttgc gcagtttctc tttggcgccc accccgaatt tctgctcctc atcgacgatg
     1261 agcaaaccca gatttttgaa ttcaacatcc ttgctcagaa ttctgtgcgt gccaatgagt
     1321 atgtcaacct ggccttcctt cagctttttc agcgtttctt tctgttcttt cgttgaacga
     1381 aatcggttca tgtattcaac gcgtgccggc aattcatcca gtcgttcttt aaacgtgcaa
     1441 taatgctgat aggccagaat ggttgtcgga acaagcaatg ccacctgttt actgtcggcc
     1501 actgctttga atgcagcccg cacggcaagc tctgttttgc cgaatccgac atcaccgcaa
     1561 atcaaccgat ccatcggaaa atcagcttcc agatcggctt taatggcttc ggtggctgtt
     1621 gcctgatcgg gagtgtcttc atagatgaac gaagcttcaa gttcgtgctg cagataagta
     1681 tcgggcgaaa aagcataccc ttttgccgca cgacgcttgg catagagctt aatcaactcg
     1741 cgcgcaatat ccttaacttt tttctttgct ttgttttttc tggtagtcca tgaagcgctg
     1801 cctattctgt caatcgaagg cggagagcca tctttaccaa tgtacttcga aatacggtgc
     1861 aaggcatgaa tgctcaccat cagctcatcg tcgtctttat acaataaacg gatggcttcc
     1921 tgaatctgcc cgttgacctc caccttctcc agtcccgcat agcgtccgat gccgtgattg
     1981 atatgcacca cataatcgcc aggttgcagg ttgacaagct cgttgatgga aagtcgctcc
     2041 ggacggctga aacgatcatg cagactgctg cggcgatatc tgttgaacag ctggtgatca
     2101 gtataaatcg ccaccttgct cagattgtcc gaaaaccctt cacccagcga aaatttcaga
     2161 atactgattt tgtcggtgat actgatgcgt ttctgcgatt caatatcttt caaaattgca
     2221 tacagacgct caatctgctg cgcgttttca gcaattatac ttattgacca cccattcgac
     2281 agacgtgaaa atacttcttc aacaaaccaa tcaaattttc ggttgactgg tggctgaggc
     2341 gaaaatgaaa aaactattct atcggaatca ttgactgaaa gcggcgttgg acaggtgtac
     2401 acctgaatga atttttcaat tgaaccagac cagccatccg catcaagaat tttttcggca
     2461 ggaatagcac cgctgttttc aagccgcaca cattcttctc taaagcgctg tgctgccgaa
     2521 ggatcattga tccagataga agtatcttcg ggaaacaaat caaaaaccgg caccagatca
     2581 tgatcgtttt gcatacgact caaatcgggc agaatctgta ttgtatcatg attcgaaata
     2641 gtaagctgcg aagcgggatc gaaagacctg atgctttcgg ccgtattgcc ggaaaaggta
     2701 attctgaacg gattttcatc agcaaaagaa aacacatcgc atataccacc acgcaaagca
     2761 aactcaccag gttcgcacac aaaatcaacc tggcgaaaat catagccggt taacacttct
     2821 tcaataaaat ccagatcaat tttttcgccg gcattcagag tcaatgaatt ttgctgcatg
     2881 aatccggcgt tgggcactcg cgtgcaaaga gctgccggcc aggtaatgag gcaggtcggt
     2941 ttattgccgt gcaacgaccg gagcgcttcg gcccgcatga gcaaatggcg cgaatccgtt
     3001 tcggccggtg catgttcttt gcgatagagt gttggaaaaa atgcaaccga tttgtcgccg
     3061 ggtggcatat ctttttgcga caacagattt tccatatcat cgtaaaacga catagcatct
     3121 tcgcggttag ccgcaataat aatctgagag cgattctgaa caaccgaatt aaacgcacag
     3181 agcaatggtg cagcagagcc cgccaatcct tcaagtgaaa tgacacggct atgcccacct
     3241 gatatttttt ctgcaagtag tttaaagcat ccggtctctg ataccggtgc caccagcggg
     3301 tgaatggctt cgctcagttt catgctgcaa aggtaattct ttcacacgtt tatgaaaaga
     3361 aatccatcgg gtataattac gcagtctcag tagaatattt ctcaaaaaat aaggagcagc
     3421 aatccaacat tccttagttc ggagagaatc gcagaacatg gaatagttgg taagaggttt
     3481 cagcatccga ctcttccatg acctccattg cattgcggac tatggaaggt cggtgtgctc
     3541 tttgtttcac caaaataatg ggagcatgta attggcatat ttgcaggcag cactgtaagc
     3601 gcaaaccaga gatgtcatcc gtggacactt tcagccaata caaatccacc cctattgctg
     3661 gtcaataagg caaaaagaaa tatactgccg gactaatccg gcagtattaa agagcaaaat
     3721 tttctgggaa aactagcggt tttcttctct tggtcttgcc tggttcacaa ccatctgacg
     3781 accgtcaacg tctttgccgt tcaattcagc aattgcacgc tgtgcatcat catcgttggt
     3841 catggtaaca aaaccaaaac ctttgctttt tccggtgaat ttgtcagtga taacaactgc
     3901 agattcaact tctccgaatt ctgaaaatac agatttcaga cgatctccgt taactttgta
     3961 attcaggttt cctacgtaaa tgttcataat gaaaaatgtt aaagacattt tgttgttttc
     4021 tggttctttt tacccgattt tcaacaaaac taccacaaat gtaaatgttt ttttcatttg
     4081 aaacactaaa tcagaaaaat taacaaattt atttattcat ccgagcgaca aatttaatcg
     4141 ccgcgtatag tccactcatc tgtatttctg acacttaagt aaaataattt tccattttta
     4201 caaaaaacat taaaatacga tttcatagat aatcgctacc tttacaaaaa aaatttatgg
     4261 catggcttaa
//
LOCUS       DAOPKE010000154         4239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_22882_length_4239_cov_12.532983, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000154 DAOPKE010000000
VERSION     DAOPKE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22882_length_4239_cov_12.532983"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1215)
                     /locus_tag="PKY63_12365"
     CDS             complement(85..1215)
                     /locus_tag="PKY63_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF01452.1"
                     /translation="MKVSAFKSGKWVNQYQYKSFLPETINHAWTIDDDRMNLLLSQAA
                     MHLGELNAFSSLIPDVDIFIKMHVQKEAMNSSRIEGTRTNIEESLNKEENIDPEKRND
                     WQEVRNYVNAMNAFVMSLNKLPLSGRLMKQTHKVLMKGVRGEAKNPGEFRSSQNWIGG
                     RSIKDAVFVPPHPHDLPDLISDLETFMNNNRLSVPELIKAGIAHYQFETIHPFLDGNG
                     RVGRLLITLQLVSHGLLQKPTLYLSDYFEKNRSEYYSRLSKVRTDNDLNGWLMFFLEG
                     VRQTSADSAQTFRKIIELRKKTEKKLMKPKKASKSAFALHDFMYRIPVFEAQDVADEL
                     GVNISTAYRMTEQFMKFGILEESTGFKRNRVFVFRPYIDLFR"
     gene            complement(1366..3162)
                     /gene="uvrC"
                     /locus_tag="PKY63_12370"
     CDS             complement(1366..3162)
                     /gene="uvrC"
                     /locus_tag="PKY63_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPF01453.1"
                     /translation="MSDFSENIKQKLATLPSQPGVYQYFDAEDTIIYVGKARNLKKRV
                     SSYFHKEHDSAKLKLLVRKIARIETMVVRSEFDALLLENNLIKQYQPRYNVLLKDDKT
                     YPWICVTDEEFPRIFSTRSKDFRGEYFGPYPSGRMVKALTDIIRRIFTLRTCRLSLTE
                     EGIAKNRFKSCLELQLGNCLAPCIGQQSRAEYIDMTIQARNILKGNLNPVIQTLKTQM
                     NEYSGLLEFEKAHKIKDKLEALTSFQARSGVTFEKLGDVDVFSVVSDEDYGYVNFLRL
                     LNGNVVQGHTYALKKKMDETDVDLLMISLGEFLSLNGNFAPLVLLPFETEIEIPKVKF
                     EVPKISDKKQLLELSLSNAKQFRIEQGKKRDMIDPERHSKRIMQTMMNDLHLKKLPEH
                     IECFDNSNFQGTDAVASCVVFRKAKPAKSEYRHFNIKSVSGPDDFASMTEVVTRRLKR
                     LLEEGSDLPDLLIVDGGKGQLSAAVEALDALNLRHVVPVIGIAKRLEEIYFPGDTLPI
                     YIDKKSESLRLIQRMRDEAHRFGITHHRNKRSKGLLKTELTGIPGIGKTLAEKLLKHF
                     GSVEALKSASEEEIQKVVGVKKAEGILKWRNS"
BASE COUNT         1213 a          922 c          839 g         1265 t
ORIGIN      
        1 tcgtttcatc ggaaaagtat ttcgggacaa atttcggaaa aaattcgttg taaaacagga
       61 acaaaacaga aatcagcaac cagattaacg gaataagtcg atataaggcc tgaaaacaaa
      121 taccctgttt cgcttaaaac ctgtggattc ctcaagtatt ccgaacttca taaactgctc
      181 cgtcatgcga tatgcggtag agatgttaac gcccagttca tcggctacat cctgcgcttc
      241 gaacaccgga atgcggtaca taaaatcatg tagcgcaaag gcagatttag aggccttttt
      301 cggtttcatc agtttctttt cggtcttttt gcgcagctca ataatttttc tgaacgtctg
      361 cgccgaatca gccgatgttt gccggacacc ttccagaaaa aacattaacc aaccgttcaa
      421 atcgttatca gtcctcactt tcgacaaccg ggaataatac tcgctccggt ttttttcgaa
      481 ataatcagaa agatataagg ttggcttttg caataatcca tgactgacaa gctgcaaggt
      541 aatcagtaag cgacctacac gcccgtttcc atcgaggaat gggtggatgg tttcaaactg
      601 ataatgagca atacctgcct taatcaattc cgggacagac agtctgttgt tgttcataaa
      661 agtttcaaga tcactgatca ggtcgggcaa atcatgcgga tgcggtggaa caaaaacggc
      721 atcttttata ctccggcctc caatccagtt ttgcgatgat ctgaattcgc ctggattttt
      781 tgcctcacca cgaacacctt tcatcagtac tttatgtgtc tgcttcatca atcgaccact
      841 gagcggcagt ttattcaatg acataacaaa cgcattcatg gcgttgacat aatttcgaac
      901 ctcctgccag tcgttgcgct tttcaggatc aatgttttct tctttattca acgattcctc
      961 aatgttggta cgtgttccct caatgcgact gctgttcatg gcttccttct gaacatgcat
     1021 tttgataaaa atgtccacat ccggaatcag cgaagaaaag gcattcaact cacccaaatg
     1081 cattgcagcc tgactcaata acagattcat tctgtcatca tcgatggtcc acgcatgatt
     1141 gatggtctca ggcaaaaagc ttttgtactg atactgattc acccatttac cggacttgaa
     1201 tgcactgact ttcattttgc aaatgtgatt atgaatgcaa atataatatt ttttattcgc
     1261 ttttttgtgc ttttttgcaa atatggcact ttttattcgc aaaatattcg caagctcaaa
     1321 ccgccgccgc gacattcgaa aatcttttaa aagaacttat tgtaatcaac tattccgcca
     1381 tttcaatatc ccctccgcct tcttcacgcc aacgaccttt tgtatttcct cttcagaagc
     1441 cgatttcagt gcttcgaccg aaccgaaatg cttcaataat ttctcagcaa gtgttttgcc
     1501 gattccaggg attccggtca gttcggtttt cagcaatcct ttgctgcgct tattgcgatg
     1561 atgagtaatt ccgaatcggt gcgcctcgtc gcgcatgcgc tgaatcaacc gaagtgactc
     1621 acttttctta tcaatataaa tgggcagcgt atcgcccgga aaataaattt cctcaagacg
     1681 ttttgcaatg ccgatgacag gaacaacgtg gcgcagattc agcgcatcca aagcttcgac
     1741 ggctgcgctc agctgaccct tgccaccatc aacaatcaac aaatcaggca ggtcggagcc
     1801 ttcttccagt aagcgtttca ggcgacgggt taccacttcg gtcatgctgg caaaatcgtc
     1861 ggggccgctc acagacttaa tattgaaatg gcggtattcg cttttggccg gcttcgcctt
     1921 gcggaaaacc acacaggaag ccaccgcatc ggttccctga aaattggaat tatcgaaaca
     1981 ttcaatatgc tccggaagtt ttttcagatg aaggtcattc atcattgtct gcatgatgcg
     2041 ctttgaatgc cgctccggat caatcatgtc gcgttttttc ccttgttcaa tacgaaattg
     2101 tttagcatta gacaatgaca attcgagcag ctgtttttta tcgctgattt tcggaacctc
     2161 gaatttcacc ttcggaatct caatctcagt ctcgaaaggc agcaacacca acggagcaaa
     2221 atttccgttg agcgaaagaa attcacccag actgatcata agaagatcca cgtcggtttc
     2281 atccattttt ttcttcagtg cataggtgtg gccctgtacg acattaccat tgagcaggcg
     2341 cagaaaattc acgtatccgt agtcttcatc cgaaacaacg gaaaatacat ccacatcgcc
     2401 caacttctcg aaagtcacac ccgatcgggc ctggaatgat gtcaatgctt caagtttatc
     2461 tttgatttta tgcgcctttt caaattcaag caacccggaa tattcattca tttgagtttt
     2521 taaagtctga ataaccggat tcagattgcc tttgagaata ttgcgcgcct gaatagtcat
     2581 atcgatgtac tcagcccggc tttgctggcc gatgcaggga gcaagacaat tgcccagctg
     2641 cagttcaaga caggatttga agcgattttt tgcaataccc tcctcagtca gtgacagtct
     2701 gcaggtgcgc aaagtaaaaa tcctgcggat gatatcagtc aacgctttca ccatgcgccc
     2761 ggatggatag ggtccaaaat actcacctct gaaatctttt gagcgcgtgg aaaatatccg
     2821 agggaattcc tcgtcggtga cgcagatcca gggatatgtt ttatcgtctt tcagcaatac
     2881 attgtagcgc ggctgatatt gcttgatgag attattctcg agcagcagcg cgtcgaattc
     2941 gcttcggacc accatggttt cgatacgcgc aatcttgcgc acgagcagtt tcagtttggc
     3001 actgtcatgc tccttgtgaa aataggacga gacgcgcttt ttcagattcc gtgccttacc
     3061 gacatagatg atggtgtctt cggcatcgaa atactgatac acacctggct gcgatggcag
     3121 cgtagcgagt ttttgtttta tattttctga aaaatcggac aaggaaataa tttgagttca
     3181 aacatgcaaa aatacgaaat cagaaatacg aaatcagaaa tacgagatac gaaatcagaa
     3241 atacgaatca aggcgatatg cgttagtggt tatgagttac gaattatgag ttataagtag
     3301 attaataact ataccctaaa cagatagatt caatgactaa aattcttgat tggaaaatat
     3361 ttaaattatt tttgtgttct tattcaaaca gaaaaaatgt ctggattctt cggttttttt
     3421 attctgatta tcacaatgat actggatttt tggctctact atgacaaaag agtcaaaaaa
     3481 cttaatttca taattcttgc cggatggctt gtttcatttg ggttcgtttt tcctttgatt
     3541 gcagctcaaa ttgaggaatt atcagtcatc aaggaaaacg aaataattga cagttatgaa
     3601 atgctttata cgattctata ctttccgata tattggggtt tgggaattat agaactgatt
     3661 gcattacttc tactctttcg aagcaaaaaa gctataaaaa aacatgatat gaagcttaga
     3721 attaagagat tccggcattt tcccaaaaag acgaatttcg aaaaacctta ttggaaggtg
     3781 tgatttgaat tgttctgtat tctgcagaat gaattccaga attaggttct aagagttttt
     3841 tcttgagtac ttcaaaatgt ttgcagtatg tctgctatta caaaggtaga gcctgttccc
     3901 ggtcaaaata tgagatatag tttctgcttc cgtgccgtga tttgccatga aaatcatcga
     3961 ggccattgct gaatgtacat ttcaggagac cccctgacgg taccgtccag gggacttggt
     4021 actctaagcc gggcaaactg aagcgaaaac aaatttgtaa atgttatgtg catgataatc
     4081 atacaaacca ttttaatttt cgaaattgag aaatttgccc cataaaatcc tgtattacct
     4141 ctttttcttt taaaggaatt ctaataaaaa taataaattt gccctttatt cgttatcaga
     4201 ccaaaatacc ggcatgagga aaagccattt attgatact
//
LOCUS       DAOPKE010000155         4214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_23062_length_4214_cov_12.147872, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000155 DAOPKE010000000
VERSION     DAOPKE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23062_length_4214_cov_12.147872"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(515..2995)
                     /locus_tag="PKY63_12375"
     CDS             complement(515..2995)
                     /locus_tag="PKY63_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF01454.1"
                     /translation="MNSQEIQSNKELEMAFEFVYRTNRNVYLTGRAGTGKTTFLNRLR
                     NECPKRMVIVAPTGVAAINAGGVTIHSMFQLPFAPFFPESAGLQNNPLNAIRYRKQKI
                     RLLKSIDLLVIDEISMVRADILDAVDSLLRRYRHSNQVFGGVQVLMIGDVHQLPPVVK
                     EDEWKMLRLYYRNMYFFSSKVFASASPVVIELQQIFRQSDLDFILLLEKIRNNGMDAN
                     TMQLLNQRYNKHFNPSAHPGYIILTTHNSQSATINDKRLGELSGEEHNFEAEVKGDFP
                     EYNFPTFKTLVLKAGAQVMFVKNDLAPEKRYYNGKIGKIVHIEDDNIVVQCPEDDDTI
                     DVGMVSWSNIRFTLNEATKEIEEEEIGSFTQYPLKLAWAITIHKSQGLTFDKVVVDAA
                     QAFAHGQVYVALSRCRTLEGLVLSSAIGYGSVITDNNVVDFNRRSEELVPDDRELEVS
                     KGQFRNEILAHLFDFSEMLNRMIHLRRLALDAGESVSSALVPDTEHTIEFLSKEIIDV
                     ASKFRRQLDALFSLAGTEDGEKHLKDRVLSAAAYFKDKLATIFDYTAYTDFDSDNMSE
                     QQELKELAEDLQKMAVIARACFDALLKDFDVFSLLRIRSDADLDFRFVSRRERSVRKD
                     SKLSSVIEYPELYNILKAWRDDLADNSNMERYRVMPNKALEEMANMLPTTLGELKKVK
                     GLGKKKIEFFGTELLKIIAKYCIEHDLKMVEFPTDEPAPKASKKKKGASAEVSLEMLR
                     DGYSVAKIALERNLTEGTVASHLLKYIESGEITVLQLLPREKLDRIATALVDYKFTSL
                     KEAKDYLGSDIEFHEIRWVMVAMQKELD"
     gene            3098..3412
                     /locus_tag="PKY63_12380"
     CDS             3098..3412
                     /locus_tag="PKY63_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01455.1"
                     /translation="MTTEEKKYAEIGLSIENAVQSQMFGKPCFKINGKAFVCFFQNEM
                     IFKLSDEAHSEALKLSKSHLFDPSGKGRPMKQWVQVPGGHMDKWPYFAIVALEYSSLS
                     KQ"
     gene            3483..4046
                     /locus_tag="PKY63_12385"
     CDS             3483..4046
                     /locus_tag="PKY63_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPF01456.1"
                     /translation="MELPEHLIQQTRLSQENVNVAVTLFERHDWPKGHALFNEGEICD
                     RIFYIEKGFVRFSHISESGKEITTWFFPDYSFLTSINSFYNTGPSDVSCVLNEDSVVY
                     TITYSQFHQLLNDYPELGKFCFAIVFELARQMVEYISSSKFLTAEDRYNELIKRHPLI
                     LQKAPLGQIASFLGITQETLSRIRAKK"
BASE COUNT         1140 a          993 c          899 g         1182 t
ORIGIN      
        1 atttctgatg ccagcggaag aattgtactt gccaaaaacc tcatcagaag ccagaatcca
       61 gagattgatc tttcagcact cgatgacggg atttattttg ttcgtgttga aatgctgaat
      121 ggtacttcga ttcagaagat tgcgaagact tcagaataat gtgctactca tgaagcatgc
      181 ggcacagagt gcagtgtaca acacacaagg tgtggcgaat agtgcattgc gcacccaaca
      241 ttttagcgtt cgcaaggcaa atgcataaaa tgggagcgga cctggaatcg ttgggtgcaa
      301 gcagccttct tcccgacgat tccacgtcag caatgcctgg ttgtcgtagc agaaggtaaa
      361 atgtcgggag gccaattccg acgcttcggt cggcgcattc tgccaattac tgaatgcttt
      421 gatgtaacgt ttgtaacgcc acgcttccag cgtgataaac tccacgacag caatgtctgc
      481 atcaaatcaa aaatctaaaa tctgctatga taaatcaatc aagctctttc tgcatcgcta
      541 ccatcaccca acgaatttca tgaaactcaa tgtcgcttcc aaggtagtct ttggcttcct
      601 ttaaactcgt gaatttatag tctaccagcg cggttgcaat gcgatcaagt ttttcgcggg
      661 gaagcaactg cagcaccgtt atctcgccgc tttcaatgta tttcagtaaa tgacttgcga
      721 cggtcccttc agtgagattg cgttccagtg cgatcttcgc aacactgtaa ccatcgcgca
      781 gcatttcgag agatacttcg gccgaagcac ctttcttttt cttcgatgct tttggagccg
      841 gctcgtctgt tgggaattca accattttca aatcatgctc aatgcaatac ttcgcaatta
      901 ttttcaataa ttcagttccg aaaaattcga ttttcttttt gccgaggcct tttactttct
      961 tcagttctcc aagcgttgtg ggcagcatgt tggccatttc ttcaagcgcc ttgttaggca
     1021 tcacccggta gcgctccatg ttcgagttat cggccagatc gtctctccac gctttgagga
     1081 tgttgtacaa ttcaggatat tctatgacgg agctcaactt tgaatctttt cgcacactgc
     1141 gctctctgcg cgaaacaaat cgaaaatcaa gatcggcatc ggaacgaata cgcagcagcg
     1201 aaaaaacatc aaagtccttc aaaagcgcat cgaaacaggc acgggcaatg accgccattt
     1261 tctgcaaatc ttcagccagc tccttcagtt cctgctgctc gctcatgttg tcggaatcaa
     1321 aatctgtata agctgtataa tcaaaaatcg tcgccagctt atccttgaaa tatgctgctg
     1381 ccgacagcac gcggtctttc aaatgcttct ccccatcttc cgttccggcc aatgagaaca
     1441 aagcatcaag ctgtctccgg aattttgatg caacatcaat aatttccttg ctcaggaatt
     1501 caatagtatg ctccgtgtcg ggaaccaatg cagatgacac actctctccc gcatccagag
     1561 ccaaacgacg aaggtgaatc atccggttca gcatctcact gaaatcaaaa agatgcgcca
     1621 gtatttcatt cctgaattga cccttcgata cttcaagttc cctgtcatcc ggtaccagtt
     1681 cctcgctccg gcgattgaag tcaaccacat tgttatcggt tatcacgctt ccatagccaa
     1741 tggccgaact cagcaccaat ccttcaagtg tacggcagcg gctgagcgcc acatacacct
     1801 gtccatgcgc aaatgcctgc gcagcatcga ccacgacctt gtcaaatgtc aggccctggc
     1861 tcttatgtat ggttatcgcc caggcgagct tgagcggata ctgagtgaag gagccgattt
     1921 cctcttcttc aatttccttt gttgcctcgt tcaatgtgaa acggatgttg ctccatgaca
     1981 ccattccaac atcaatggta tcatcatctt cgggacattg cacaacgata ttatcgtctt
     2041 cgatgtgaac aatctttccg atttttccat tgtaataacg cttctccggc gccagatcat
     2101 ttttgacaaa catcacctgt gcacccgcct tcagaacaag tgttttgaat gtcggaaagt
     2161 tgtattcggg aaagtcgcct ttcacttcgg cttcgaaatt gtgttcttcg ccgctcagct
     2221 ccccgagccg tttgtcgttg atggtagctg attgtgaatt atgtgtggtc agaataatat
     2281 aacccgggtg ggccgatgga ttgaaatgct tgttatagcg ctgattcagc aactgcatgg
     2341 tgttggcatc cattccgttg ttacggattt tttcaagcag cagaataaaa tcaaggtcgc
     2401 tctgacgaaa aatctgttgc agctcaatca ctacaggact tgcactagca aaaaccttgc
     2461 tgctgaaaaa atacatgttg cggtaataca accgcagcat tttccattcg tcttccttca
     2521 caacaggcgg caactgatgc acgtctccga tcatgagtac ctgcactccg ccaaacacct
     2581 ggttggaatg ccggtagcgg cgcagcagtg aatcaaccgc atcaagaata tcagcgcgca
     2641 ccatacttat ttcatcaatt accagcagat cgatgctttt cagcaatctg atcttttgtt
     2701 tgcgataacg gatggcgttg agcggattat tctgaagtcc tgccgattca ggaaaaaacg
     2761 gagcaaacgg cagttgaaac atactgtgaa tggtcactcc gccggcattg atggcggcaa
     2821 ctcctgtcgg agccacaatc accatccgct tgggacattc attccgcaaa cgattcagaa
     2881 aagttgtttt accggttccg gcacgaccag tgagatagac attgcggttt gtgcggtaca
     2941 caaactcaaa cgccatctca agctctttgt tggactgtat ttcctgcgaa ttcacaggtc
     3001 aaagttaagg aaaatccata tgcaaagtag tgcgaagttt ataaacttcg cactacttta
     3061 taatttttca ttacttttga aacagaagca atcaaatatg accaccgaag aaaagaaata
     3121 cgccgaaatc ggcctcagca ttgaaaatgc agtgcagagt caaatgtttg gaaagccctg
     3181 tttcaaaata aatggaaaag catttgtgtg cttttttcaa aacgagatga tcttcaaact
     3241 aagtgatgaa gcgcattccg aagcattgaa actttccaaa tctcaccttt tcgatccctc
     3301 gggcaaaggc agacccatga aacaatgggt gcaggtgccg ggcgggcata tggataaatg
     3361 gccatatttt gccattgttg ctttggaata ttccagcttg tcgaaacaat aaattcgcat
     3421 ccactttgtg tatttttgta caataaaagt agcaatcggc tgttgtgatt aaaaattaaa
     3481 tcatggaact tccggaacat ctgattcagc aaaccagact ttcacaggaa aatgtgaatg
     3541 ttgctgtgac tttgtttgaa cggcatgact ggcctaaagg gcatgcgctg ttcaatgaag
     3601 gagaaatatg cgacagaata ttttacatag aaaaaggatt tgtacggttt tctcatattt
     3661 ctgaatccgg caaggaaatc acaacgtggt ttttcccgga ttattcgttt ctgacatcaa
     3721 taaattcttt ttacaacact ggcccatctg atgtaagctg tgttcttaat gaggattctg
     3781 tagtttacac aatcacttat tctcaattcc atcaattgct aaacgattat cctgaattgg
     3841 gcaaattttg tttcgccatc gtatttgaac tggcgcgtca aatggtggaa tacatcagca
     3901 gcagtaagtt tctgactgca gaagaccgct ataatgagct gataaagcgc catccgctca
     3961 ttctgcaaaa agccccgctt ggacagatcg cctcatttct tggcatcacg caggaaaccc
     4021 tgagccgcat cagggcgaag aagtaggggc tgctgtaaga ttgagattaa ggtcaaggtt
     4081 gagctatggc aagctgtttt ttgtgagcta cgaaaatatt ttctttcttt ttctttgatt
     4141 aataaaacaa gcagctttac cttaccgctt tatggcattg agtgtatagg gattgtcttg
     4201 gatggtgcag tgca
//
LOCUS       DAOPKE010000156         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_23425_length_4173_cov_12.837300, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000156 DAOPKE010000000
VERSION     DAOPKE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23425_length_4173_cov_12.837300"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..1863)
                     /locus_tag="PKY63_12390"
     CDS             complement(361..1863)
                     /locus_tag="PKY63_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF01457.1"
                     /translation="MENILPIHLQEVIFSSSDSQLSKQISGLEKAGKLRNIAPRIYTP
                     NFTDSPEKIIRRNLFTILGKLYPGALLSHRSALEFQPTSAGHIFLTYTYTKKIKLPGI
                     TLRFMEGPAPMDGDNRLSGELYASQTERALLENLQISRQVGPESKTLTLPEIEEKLET
                     VIRVKSETGLNELRDRARVVAEKLEMKREFEKLNQLISALLTTHPSKILSSPVALARA
                     FGNPYDPARLTIFEKLFIELKQKEFADQTEKNISNQSFRNFAFFEAYFSNYIEGTEFE
                     LKDARQIIESETPMPARDEDSHDILGTYKLVSNKTEMSIIPASPDELLEILSYRHKVL
                     LNARTSMNPGQFKDKNNRAGDTFFVDHTLVKGTLIKGFDFYQALTHPFAKAVYMLFLV
                     SEVHPFADGNGRIARVMMNAEFVRNEQTKIIIPTVYRDDYMIALRRLSRQQDPSAYIR
                     MMQRAWTFSATISGDQIDRMEKHLEASNAFKEHDKAKLKIISQGNTDLGK"
     gene            complement(2029..3339)
                     /locus_tag="PKY63_12395"
     CDS             complement(2029..3339)
                     /locus_tag="PKY63_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPF01458.1"
                     /translation="MVDYAFVKIWNTLAGAVTWDSKRNLASFQFDKKFLNNNWDLSPL
                     MMPIHNGDRIYNFPELLPSKDSTEDPFKGLPGLLADSLPDKYGNLLIDRWLAQNGRAP
                     QSMNPVEKLCFIGSRGMGALEFEPAQIQNSKYTFSLEIKSLVDIAQKMLSDREHFEAR
                     LDADEQQAMEQILKIGTSAGGARPKALIAFNEHNHEVRSGQTRVPQGFSHWLLKLDGV
                     SGIQFGNTHGWGRIEYAYHLMAAACGIEMTECRLLEENGRAHFMTKRFDREGSETKHH
                     ILTLCGINHFDYGNIFSYGYEQVFQTMRMLKLPYSQAEQMFRRMVFNVLATNCDDHTK
                     NFSFRLKKDGVWELAPAYDVCYAYDPSNLWVNQQSLSINGKHREINKNDLMTIARANN
                     IKKGEQIIDEINNIVKNWSHFAVEAKVNIRMRKEIESNLHVLND"
     gene            complement(3333..3686)
                     /locus_tag="PKY63_12400"
     CDS             complement(3333..3686)
                     /locus_tag="PKY63_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF01459.1"
                     /translation="MMEFTNTKWIAMSDKAIIASIGDFIRQKRLQVNKTQLQLATDAG
                     LNRSTVVQIERGESVTLSSLIQILRVLDLLHLLNIFTTDDTISPLEYAKMKEQKRLRA
                     TKKNKASGLNEETEW"
BASE COUNT         1118 a          882 c          822 g         1351 t
ORIGIN      
        1 aatgaaaaaa tgatttcgcc ccggtcctgt aaaatttgtg ttgcttgttg ttgtttgact
       61 tcggatacgt tttgcgcatg cgttacggct gcaaaaaagg aaattaaaag acatagaaaa
      121 ataaacctgt actccataat tttaaaattt aataatcaga caaatataaa aaacaattgg
      181 ttgcgaatat ttgtattatt tttataaata caccaacgct tttatatcgt gtctgatgtg
      241 gggtatattg acgatgaaac ggatgatttt acaggtttga ggaatgaagc aacagctttt
      301 gcatttattt gttccccatc ggtaattgat gcgaaatcaa tatgaatcga tgtgagctgc
      361 ttatttcccc aaatcagtat ttccctgaga aataattttc agctttgctt tatcatgttc
      421 tttaaatgca ttgcttgcct cgaggtgttt ttccatacgg tcaatttgat ctccggaaat
      481 ggttgcactg aacgtccatg ctctttgcat catgcgtatg tatgccgaag gatcttgctg
      541 gcgcgacaga cgcctcaatg caatcatata atcatctctg taaacggtag ggataatgat
      601 ttttgtttgc tcgtttcgca caaattcggc gttcatcatt acccgtgcaa tacgcccgtt
      661 gccatccgca aaggggtgaa cttcgctcac taaaaacagc atgtaaacag cttttgcaaa
      721 aggatgcgtt aacgcctgat agaaatcaaa acccttaatc aatgtacctt ttaccagcgt
      781 gtggtcaaca aaaaaagtat caccggcgcg gttgttctta tctttaaact gtcccggatt
      841 catcgatgtg cgcgcattca gcaatacttt gtgccggtat gaaagtattt ccagcagctc
      901 atcggggctt gccggaataa tgctcatttc agttttattg ctcacaagtt tgtatgtgcc
      961 cagtatatcg tgcgaatcct catcacgggc gggcattggc gtttcgcttt ctataatttg
     1021 ccgtgcatcc ttaagctcaa attcagttcc ttcaatgtaa ttggaaaaat acgcttcaaa
     1081 aaaagcgaag ttccggaacg attggttgct gatgtttttt tcagtctgat cggcaaactc
     1141 tttctgcttg agttcaataa ataatttttc aaaaatggtc aatctggccg gatcgtaagg
     1201 attgccaaat gcccttgcca aagctaccgg cgatgatagt atttttgaag gatgtgtggt
     1261 aagcaatgca ctgatgagct ggtttaattt ctcaaactct cttttcattt ccagcttttc
     1321 agctactact cttgctctgt ctctcagctc atttaatccg gtttcgcttt tcacccggat
     1381 gacagtttcc agtttttctt caatttcggg cagggtgagt gttttagatt cagggcctac
     1441 ctgacggctt atctgtaagt tttcgagtaa tgccctttcg gtttgcgaag catataattc
     1501 acccgaaagc cgattgtcac catccatggg ggcaggccct tccataaacc gcagcgttat
     1561 gcccgggagt tttatttttt ttgtataagt ataggtgaga aaaatatggc ctgccgatgt
     1621 gggctgaaat tcaagggccg atctgtggct gagcagagct ccgggataca attttcctaa
     1681 tatggtaaac agatttcgcc ggatgatttt ttcggggctg tcggtaaaat tcggcgtata
     1741 aatcctgggc gcaatatttc ttagctttcc ggccttttcc agcccggaaa tttgtttgct
     1801 taattgcgaa tcgctggaag aaaaaataac ttcctggaga tgtatgggta aaatattttc
     1861 cattatacca gcttatttct gcaaatatat caaatatttt ccactaaaac caccttttta
     1921 gaaaataatt tccattataa atgcaaatac agataaataa tttccactat aaatcatttt
     1981 atgggaaaat attttccagt aaaaggcaga ttatactttg caaagaagtc aatcattcag
     2041 tacatgcaga ttcgattcta tctctttacg catacgaata ttgacttttg cctccactgc
     2101 aaaatggctc cagttcttga caatattatt tatttcatct atgatctgct ctcctttttt
     2161 gatgttattt gcccgggcaa ttgtcataag atcattttta ttgatttccc ggtgcttgcc
     2221 attgatgctt aatgattgct ggtttaccca caaattggaa ggatcgtatg cataacacac
     2281 atcataggcc ggagctagtt cccacacacc gtcctttttc aggcgaaatg aaaaattctt
     2341 tgtatgatca tcgcaattgg ttgccagtac attaaaaacc attcttcgga acatttgttc
     2401 agcctgagag tagggaagtt taagcatgcg catggtctgg aacacctgtt catatccata
     2461 actgaagata tttccataat caaaatgatt gattccacac aatgtcagaa tatgatgttt
     2521 tgtttcactg ccttcgcggt caaaacgttt ggtcataaag tgagcgcggc cattttcttc
     2581 gagcaatctg cattcggtca tttcaatgcc acatgcagca gccatcagat gatatgcata
     2641 ttcgatgcga ccccagccat gtgtattgcc aaactgaatg ccgctcactc catccagttt
     2701 cagcaaccaa tgcgaaaagc cctgtggaac tctggtttgt cccgacctga cttcatggtt
     2761 gtgttcatta aaagcaatca atgcctttgg tctcgctcct ccagccgaag taccgatttt
     2821 aagaatctgc tccatggctt gttgctcgtc cgcatctaat cgggcttcaa aatgttcacg
     2881 atccgagagc atcttttggg caatatcgac cagacttttt atctctaagg agaaagtata
     2941 tttgctgttt tgaatctgag ctggttcaaa ctccaatgcg cccattccgc gtgagccaat
     3001 gaaacaaagt ttctccacag ggttcatgct ttgcggagct cttccgtttt gcgccagcca
     3061 tcggtcgatt aataaattcc cgtatttatc gggcaaagaa tcggcaagta aaccgggcaa
     3121 tcctttaaat ggatcttcgg tgctgtcttt cgatggcaat agttcaggga aattataaat
     3181 gcggtctccg ttgtgtatgg gcatcattaa tggcgacaaa tcccagttgt tgttcagaaa
     3241 ttttttgtcg aactgaaagc tggccagatt tcttttactg tcccaggtta cagcacctgc
     3301 caatgtattc catattttta caaaagcata atctaccatt ctgtttcttc atttaatccg
     3361 gacgccttat tctttttcgt ggcccgtaac cgtttctgct ctttcatttt tgcatactcc
     3421 agcggactta tagtgtcgtc ggtggtaaaa atattcagca gatgtaataa gtcaagaacc
     3481 cgcaaaatct gtatcagcga agatagcgtt accgattctc cgcgctcaat ctgtacaact
     3541 gtcgatcggt ttaatccggc atctgtagcc aactgcaatt gcgttttatt cacttgcagc
     3601 cgcttttgcc tgataaagtc acctatcgaa gcaataattg ctttatcgct catggcaatc
     3661 cattttgtgt tggtaaattc catcattatg ggttgtattt gtacttaacg ctggtaattt
     3721 ccaacaaata tacaaaataa attcgaataa gatgcaaaac acattaagaa aagatgacaa
     3781 aaaccatcat taagacgaaa aaagagcctt aatgttggaa aaaagtgact attccggtcc
     3841 aatcggcgcc accgatttcg aagcactgtc ctgtcaagtt acacgaaagt tcattagatg
     3901 cagaaatgct gaaataccca ctatctcggc aataaaatat accgcctgtt atgcactggc
     3961 ggttcttgtc tgtccgtagg ttcctgtcaa attgcacttt catattaaac ttctcattcg
     4021 tttcattttc ctttactgtc tgtgttgccc gtgttttggc gtgttttaaa tttttttctt
     4081 tctaaagcgt gggagaaaaa ttttgaaatt cttctcttgg gctggaacag acacactctt
     4141 tgctaagttt tgttttgtgg gtcggctggc gcg
//
LOCUS       DAOPKE010000157         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_23986_length_4101_cov_10.602323, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000157 DAOPKE010000000
VERSION     DAOPKE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23986_length_4101_cov_10.602323"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..2668)
                     /locus_tag="PKY63_12405"
     CDS             complement(284..2668)
                     /locus_tag="PKY63_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPF01460.1"
                     /translation="MKTTSKIVSKIKGTNVPPDFARYIRRMWRTFFVSAIVLYLFFIG
                     VSFGILGPIPSFEQLENPSNNLASEIYSSDQQLLGKFYVENRTNVEYQQLSPFLVQAL
                     IATEDIRFEKHSGVDARGIIRVIVKTIAGGNRSSGGGSTITQQLAKNLFPRDVNMSKI
                     ELVKTKFKEWVTAIRLERNYTKEEIIAMYLNTVDFGSQSFGIKSAAKTYFDKTPDSLS
                     IQESAMLVGMLQAPSKYNPVRNPKNAMKRRFVVLGQMAKYGYISMDQLDSLKDLPLST
                     GKYKLQDHKSGMATYFREYLRKIMNESEPERNDFPNDEEFQIANNQWDNNPLYGWCNK
                     NTKPDGTKYNLYTDGLKIYTTIDSRMQLYAEQAVSEHLGKTLQPQFNREVKHARYAPF
                     TSLHSKEEVDKLLELSKRRSDRYIQLKNDGASKEQIDKVFNEKVPMRLFSWKGEFDTV
                     MTPMDSIRYYKWFLHAGFMAVEPQTGYVKAYVGGINYKYFKFDHVCLSRRQVGSTFKP
                     FVYSVAMADGQFSPCTKIPMIPVTIDLGDGKTWSPRNSGKVKEGEMVTLRYALAGSIN
                     WASAYLIKRSSPVAVIRLARKMGVTSPIPPVYAICLGVAELTVYEMVGAQTTYVNKGI
                     YTEPIFVTRIEDKDGNVLSDFVPRRQEAMSEEAAYLMIELMKGVVDGGTSGRIRSVYN
                     IKHPIAGKTGTTDDNSDGWFMGLTPELVAGCWVGAEDMQVHFISTGLGQGANMALPIW
                     AIFMQKVYADKTINLSTEDFDKPSKPLSVEIDCKKYAEEHPEENVVKHGLNPFQ"
     gene            complement(2671..3138)
                     /locus_tag="PKY63_12410"
     CDS             complement(2671..3138)
                     /locus_tag="PKY63_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPF01461.1"
                     /translation="MKRYLFIFSLLSLFFLFGCDDSLYNKMKEIPDARWDMNYPAKFD
                     VEVSDTAALYDFYVLIRHNTDYTHSNLYTFVTTTMPGDSITRDTIEFILAEPDGRWIG
                     EGSGYLRTNEVLISRKFAFPRAGLYSFEFQQAMRDTLLSGITDFGICISPTNL"
     gene            complement(3138..>4101)
                     /gene="ricT"
                     /locus_tag="PKY63_12415"
     CDS             complement(3138..>4101)
                     /gene="ricT"
                     /locus_tag="PKY63_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPF01462.1"
                     /translation="NMGTALQFDCVEVRFKNNHKDFFRLSQGLTVTAGDVVAVEASPG
                     HDIGVISMAGPEVLRQMKRKKANPDDPQMKKVYRKAKPADIEKWYQAIEREFSTMRRT
                     LSIIRELRLNMKLNDVEFQGDNTKAIFYYTAEDRVDFRELIKIMADEFKIRIEMRQIG
                     ARQESGKLGGIGTCGRELCCSKFIHQFQSVTTNAARIQQLSLNPQKLAGLCGKLKCCL
                     NFETAVYQEILKSFPDHNIALKTKKGLATHVKSDIFKNLMWYSYTNSPANLMAVPVEN
                     VAKVQKMNKNNQIPDNLEDFAISLENKMEDDRVVGKDDISKLAD"
BASE COUNT         1173 a          947 c          781 g         1200 t
ORIGIN      
        1 tcttcgtctt gtcgcctcaa atctggcgcg cctcagactt tttcagcagg ccctatttat
       61 gggctacaga tatatgactc ctccggagtc ttccaaaaat gattggattg atgaataatt
      121 gttattgtgg tattacctgt ccgactgcag gcgggtccgt gaatatttgg caaatctgtt
      181 tcagacaaat gtcatacatc tgtcgggttt tgtgcaatga aatgtactat caaaattttg
      241 aactgttttt ctgaaattat tgcagaggtt cagaatttta attctactga aacggattta
      301 atccgtgttt tacaacattt tcttcggggt gttcctccgc atatttctta caatcaattt
      361 cgacagaaag aggtttggat ggtttatcga aatcttcggt tgaaagattg atggttttat
      421 cggcatacac cttctgcata aaaatagccc agataggcaa agccatattg gctccctgtc
      481 ccagtcctgt acttataaag tgcacctgca tgtcttcagc tccaacccag cagccagcta
      541 ccaactcagg agtgagaccc ataaaccatc cgtcgctgtt atcatcggtg gtaccggttt
      601 ttccggcaat gggatgctta atgttatata ctgaacgtat gcgccctgag gtaccaccat
      661 caaccactcc tttcatcagc tcaatcatca ggtaggccgc ctcttcgctc atggcttcct
      721 gacgacgggg tacaaaatcc gaaagcacat ttccatcctt atcctcaatg cgggtaacaa
      781 aaatcggctc ggtatagatt cctttattta cataggttgt ctgagcaccc accatttcat
      841 aaacagtgag ttcggcaaca ccaagacaaa tggcataaac cggcggaatg ggactggtaa
      901 cgcccatttt gcgtgccaga cgaattacag ctacaggcga agaacgttta atcagataag
      961 ccgaagccca gtttattgat ccagccaaag cataacgcaa agttaccatt tcgccctcct
     1021 tcactttgcc ggagtttctt ggcgaccacg tttttccatc gcccaaatca atggttaccg
     1081 gaatcatcgg aatttttgtg cacggcgaaa actgtccatc agccatggca acagaataga
     1141 caaatggctt aaaggtagaa cccacctgac gcctgctcag acaaacatga tcaaatttaa
     1201 aatacttata attgatgccg ccaacatatg cttttacata tccagtctga ggttcaaccg
     1261 ccataaaacc agcgtgtaaa aaccatttgt aataacgaat agaatccatt ggagtcatta
     1321 cagtatcaaa ctcacccttc caactaaaaa gacgcatggg aactttttca ttgaaaacct
     1381 tgtctatttg ctctttcgaa gccccatcat ttttcaactg aatataacga tcggaacgtc
     1441 gtttggacaa ttcgagcagc ttatcaactt cttccttgct gtgtaagctg gtaaacggag
     1501 catatcgtgc atgtttcacc tctctgttaa actgcggctg caaggtcttt cccaggtgtt
     1561 cactaacagc ttgctctgca taaagctgca tgcgcgaatc aatggtggta tatattttca
     1621 atccatcggt atagagatta tattttgtac catcgggctt ggtgttttta ttacaccacc
     1681 catacaaagg attgttatcc cactgattat ttgcaatctg aaactcttcg tcatttggaa
     1741 aatcatttcg ttctggctca gattcgttca tgattttacg cagatattca cgaaaataag
     1801 ttgccattcc tgacttatga tcctgcagct tatacttacc cgtactcaaa ggcagatcct
     1861 tcaatgaatc gagctgatcc atggaaatat agccatattt agccatttga cccagaacca
     1921 caaaccggcg tttcattgca tttttcggat tacgcacagg attgtatttg gacggggcct
     1981 gtaacatacc cacaagcatg gcactttcct gaatactgag agaatcaggc gttttatcaa
     2041 aataggtttt agctgcagat ttaataccaa aagactgact gccaaaatcg accgtattga
     2101 gatacatggc aatgatttcc tccttggtat aattccgttc aagtctgata gctgttaccc
     2161 actccttaaa tttggtcttt accagttcaa ttttcgacat gtttacatcg cgtgggaaga
     2221 ggttttttgc cagttgctgg gtaattgtac taccaccacc actgctacga ttgccacctg
     2281 caatggtttt tacaataacc ctgatgatgc cacgcgcatc aactccggaa tgtttttcga
     2341 atcgaatatc ttccgttgca attagtgcct gaaccaaaaa gggcgaaagc tgctgatatt
     2401 cgacatttgt gcggttttca acataaaact ttcccaaaag ctgctgatcg ctggaatata
     2461 tttcagatgc aaggttgttg cttggatttt caagctgctc gaaagatggt atcggaccaa
     2521 gaattccgaa agaaacgcca ataaaaaaca aatacaaaac aattgctgaa acaaaaaaag
     2581 tacgccacat tcttcggata taccttgcaa aatcaggtgg cacatttgtt cctttgattt
     2641 tagacactat tttgctcgtt gttttcatat ttataaattg gtcggagaaa tacagatacc
     2701 aaaatccgtt ataccagaca gaagagtgtc gcgcatggct tgctgaaact cgaatgaata
     2761 caaaccggct ctgggaaaag caaatttgcg gctgatcagc acctcgtttg ttctcagata
     2821 tccactgccc tcaccaatcc aacgaccatc gggctcggca agaataaatt caatggtatc
     2881 acgtgtaata ctgtcgccag gcattgtagt ggttacgaat gtataaaggt tgctgtgcgt
     2941 ataatccgta ttatgcctga tcaacacata aaaatcatac aaagctgcgg tatcagaaac
     3001 ctcaacatca aatttggccg gataattcat atcccagcgg gcatcgggta tttctttcat
     3061 tttgttgtac aaggaatcat cacaaccaaa caaaaaaaac aaactaagca gcgaaaaaat
     3121 gaacagatac ctcttcatca gtcagccagt tttgaaatat catccttccc aacaacgcga
     3181 tcatcttcca ttttattttc aagactaatg gcaaaatctt caagattgtc gggaatctga
     3241 ttgtttttat tcattttctg aacctttgca acattctcaa caggcaccgc cataagattg
     3301 gccggcgaat tggtgtatga ataccacatg aggtttttga aaatatcact tttaacatgt
     3361 gtagccaggc cttttttcgt cttcaaagca atattatgat ccggaaacga tttcaggatt
     3421 tcctgataaa cagcagtttc aaaattcaga cagcatttaa gtttaccgca taagccggcc
     3481 agtttttgcg gattaagcga taattgctga atccgggcag cgtttgttgt tactgattga
     3541 aactgatgaa taaatttgct gcaacacaat tcgcggccac aggtgccaat accgcccaac
     3601 tttcctgatt cctgacgggc acctatctgg cgcatttcaa tgcgtatttt gaattcatcg
     3661 gccataattt ttataagctc ccgaaaatcg acccggtctt cagctgtata ataaaaaatg
     3721 gctttggtat tatcaccctg aaattcaaca tcattgagct tcatattcaa acgcagttcc
     3781 ctgataatgg agagtgtccg gcgcatcgta gaaaattcgc gctctatggc ctgataccat
     3841 ttttcgatat cggccggctt ggctttccga taaaccttct tcatctgagg gtcatcggga
     3901 ttggctttct tgcgtttcat ctgacgcaaa acctcaggcc ctgccattga aatgacacca
     3961 atatcatgac ccggagatgc ttcaacggca accacatccc ctgctgttac agtcagtccc
     4021 tgactcagac ggaaaaagtc cttatgatta tttttaaatc tgacctcaac acaatcaaac
     4081 tgcaaggcag ttcccatgtt t
//
LOCUS       DAOPKE010000158         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_23994_length_4100_cov_13.143634, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000158 DAOPKE010000000
VERSION     DAOPKE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23994_length_4100_cov_13.143634"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKY63_12420"
     CDS             complement(<1..569)
                     /locus_tag="PKY63_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPF01463.1"
                     /translation="MKIPFLKTGDTIALAAPSRKVVSLDFAPLENHLVEIGFKVVHAG
                     NLCLADNQFAGTDAERISAMNQLFSNPEVKAVIAVRGGYGAARIVDQLKWNALLQHPK
                     WLCGFSDFTVLLNHQFACNDLPALHSDMAVHFGQNGYEDNFSSLLDALMGKSISISVS
                     KHPFNRQGNAKGILVGGNLSVLYSMLGSAS"
     gene            complement(572..1144)
                     /locus_tag="PKY63_12425"
     CDS             complement(572..1144)
                     /locus_tag="PKY63_12425"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPF01464.1"
                     /translation="MIIENVVVSDELKDVFFRCPLDLCHGACCVEGDAGAPLTDEEIS
                     IIEDLTETILPLMDEDGKNVMRNIGSFDYDMSGKMVTPLKPNEECIYLIWENGHTVCV
                     FEKLFNEGKIDFQKPISCHLYPIRVVEDGAFEKLLLHRWRICEDAYIVAKKEGVRLLE
                     FVKVPLIRKYGVQWYNRLMIKCGFDPHKTE"
     gene            complement(1148..3763)
                     /locus_tag="PKY63_12430"
     CDS             complement(1148..3763)
                     /locus_tag="PKY63_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPF01465.1"
                     /translation="MRWIFLFLMFLSAAAHAQMSDDFSDGNFTSNPVWSGDAANYIIS
                     SSTAIPSSMHPGLQSTNTVADTTYLSTPFNVTLADSMEWVFWAKMSFNPSVNNFGRVY
                     LTSNSATLSSDLSGYYVALSHNSVDRLHLVRQDGSTHTVLITGNVANFNKTTNEVRVK
                     VKRDNSGNWTLMSDTLGGDNFAIEGVVNDVTYNSSTDFGIFTQYTVSNAGKFYFDDFY
                     AGPVQVDTVPPTVLKVEFIGPTSIDVYFSEFVSATTASTISNYIIDIGIGSPLVATPD
                     ASDATLVHLILANSVLDGIMYNLTVSGVEDLSGNPMLTQIIPFAHYTPHAFDVVINEI
                     MADPSPVVLLPEFEYVELYNTTSLPVNLENFVLRIGTTDKILGDVTIDPHGFVILCDD
                     GAQSLFESYGNTFSFTTFSVSNTGVNITLLDPLGSVISFAEFTEDWYNNSFKAEGGWS
                     VEQIDPLNPCLGKYNWTSSNDNKGGTPGAINSVNAVNSDVVNPELLRASLENDSTVHL
                     WFSEPMDSSTVLNPFNYTISNGLSVFGVPTGFAPQYQSVILTLDQPVQYGIIYTVTIT
                     DTLTDCVGNPLAVSSSVRFAIADSLEVGDVVINEVLYDPVVGVDDYVELINRSNKIID
                     LRFVTLATLDDSLLIDDQAYIAPNGFLLFPGEYVAFTETVDEVINYYNPIAPNQILEI
                     SDMPAYSNEDGIVILATAAGATLDMLEYNVNMQDPILESVDGVSLERIDFDRPAADST
                     NWHSAASTVNYGTPGYKNSQYSKYEGGSNITVSPETFSPDNDGYNDVLNIAWQFDHTG
                     IICNMKIFDSNGRFIKNIMQNAPIGPEGVISWDGKDENGETAPIGIYLIIADIFASDG
                     YTDKIKTVFVLAGKF"
BASE COUNT         1297 a          896 c          817 g         1090 t
ORIGIN      
        1 gaggcagagc ctagcattga atacaacaca ctcaggttgc cacccacaag aattcccttt
       61 gcatttccct gtctgttgaa cggatgcttt gaaacgctga tggatattga ctttcccatt
      121 agcgcatcaa gtaaggaaga aaaattgtct tcatatccgt tttgaccgaa atgaaccgcc
      181 atatcggaat gcagcgccgg aaggtcatta caggcaaact gatgattgag taatacagta
      241 aaatcgctga agccacacaa ccatttcgga tgctgcaaca atgcattcca tttgagctga
      301 tcaacaatgc gggcagcacc atatccacca cgcacagcaa taacagcttt aacttcagga
      361 ttggaaaaca actgattcat tgctgaaata cgctccgcat cagtaccagc aaactgatta
      421 tcagccagac aaagatttcc agcatgaacg actttaaaac caatttcgac aagatgattt
      481 tcaagcggag caaaatcaag cgatacaact tttcgtgaag gagctgcaag tgcaatggtg
      541 tcgccggttt tgagaaacgg tattttcatt tctattcagt tttatgtgga tcgaatccac
      601 attttatcat cagacggttg taccattgaa cgccgtattt tcggatcagc ggaactttta
      661 caaattccag aagccgcacg ccttctttct ttgcaacgat gtaagcatct tcacaaatac
      721 gccaacgatg gagaagaagt ttttcgaatg caccgtcctc gaccacacgg atcggatata
      781 aatgacatga aatgggtttc tgaaaatcga ttttcccttc attgaacagt ttctcgaaaa
      841 cgcagactgt atgcccattt tcccagatca gatagatgca ttcttcgttc ggtttaagcg
      901 gtgtaaccat ttttccggac atgtcataat cgaacgaacc aatgttacgc atcacatttt
      961 tcccatcctc atccattaaa ggaaggatag tttcggttag atcttcaata atactgattt
     1021 cttcatcggt cagcggagca ccggcatcac cttccacgca gcaagcgcca tgacacaaat
     1081 cgagaggaca gcgaaaaaag acatctttca actcgtccga taccacaaca ttttcaataa
     1141 tcatcgctta gaattttcca gccagcacaa agaccgtttt gattttatcg gtataaccat
     1201 ccgatgcaaa aatatcagca ataataagat aaattccaat cggagccgtt tctccatttt
     1261 catctttgcc atcccacgaa attacacctt cagggccaat tggagcattc tgcatgatgt
     1321 tttttataaa gcgtccattg ctgtcaaaaa ttttcatatt gcatataatg ccagtatgat
     1381 cgaattgcca tgcgatgttc agcacatcgt tgtagccatc gttgtcaggt gaaaaagttt
     1441 ccggagacac tgtaatattg ctcccgccct cgtatttcga gtactgactg tttttatatc
     1501 caggtgttcc gtaattcact gttgatgcag cgctgtgcca attggttgaa tcagcagctg
     1561 ggcgatcgaa gtcgatgcgc tcgagtgaaa caccatcgac cgattcgagt attggatcct
     1621 gcatatttac attgtattca agcatgtcaa gtgttgcacc ggcagctgta gccaaaataa
     1681 caatcccgtc ttcgtttgag taagcaggca tatcggatat ttcaagaatc tgattgggtg
     1741 caatcggatt ataataattg atcacctcat cgactgtttc agtgaaagca acgtactcgc
     1801 ccggaaaaag caggaagcca ttgggtgcaa tataggcctg atcgtcaatc aacaatgaat
     1861 catccagagt ggcgagtgta acaaaacgca aatcaataat tttattacta cggttgatta
     1921 attcaacata atcatcaaca ccgaccacgg gatcatataa aacttcgttg ataacaacat
     1981 caccaacttc gagtgaatcg gcaatggcaa atcgcacgct actgctgact gcaagcggat
     2041 ttccgacaca atccgttaaa gtatcggtaa ttgtaaccgt ataaataatt ccatattgaa
     2101 ccggttgatc aagagtcaga ataacggatt gatattgcgg tgcaaaccct gttggcacgc
     2161 caaatacaga caagccatta ctgatggtat aattgaacgg attgagaaca gtactgctat
     2221 ccatcggttc gctgaaccaa aggtgcacgg tactgtcgtt ttcgagtgac gcacgtaata
     2281 attccggatt aacaacatcg ctgttgaccg cattcacgct attaatagcg ccaggcgttc
     2341 cacctttatt atcgtttgat gatgtccaat tatattttcc cagacaagga ttcagcggat
     2401 caatctgttc gaccgaccaa ccaccttcgg ctttaaatga attgttatac cagtcttcgg
     2461 tgaattcggc aaaagaaatc acggaaccga gcggatcaag caatgtaata ttcacaccgg
     2521 tatttgaaac ggaaaaagta gtaaatgaaa acgtatttcc ataactctca aacaatgatt
     2581 gcgcgccgtc atcgcaaaga ataacaaacc catgcggatc gatggtaaca tcgcccagta
     2641 ttttgtcggt agtacctatg cgtaaaacaa aattttcaag attaactggc aaactggttg
     2701 tgttgtacaa ttcaacgtat tcaaattccg gaagcaaaac aacgggagaa ggatcggcca
     2761 taatttcatt gataaccaca tcaaacgcat gaggagtgta gtgagcaaac ggaataatct
     2821 gtgttaacat cggatttccg ctaagatcct caactcctga aacagtaaga ttatacataa
     2881 tcccatccaa aacagaatta gcgagaatca aatgcacgag cgtagcatcg gaagcgtcgg
     2941 gagtggccac caatggcgat cctattccga tatcaatgat ataattcgaa atggtggaag
     3001 ctgtcgtggc acttacaaac tcactaaaat aaacatcaat actggttggg ccaataaact
     3061 caactttcag taccgtagga ggtactgtat caacctgcac cggacctgca taaaaatcat
     3121 cgaaatagaa tttgccagca tttgagacag tatattgagt aaaaattcca aaatcagtgg
     3181 acgaattata tgtaacatca ttcacaacac cttcgatggc aaaattatca ccccccaaag
     3241 tatcactcat caaagtccag ttaccgctgt tatcgcgttt gacttttacc cggacttcgt
     3301 tggtggtttt attgaaattg gcaacattac cagtaatcag cacagtgtgt gtacttccat
     3361 cctgccgcac cagatgcagt cgatcgacgc tattatgcga caatgcgaca taataacccg
     3421 acaaatcaga agaaagagtg gccgaattag acgtcagata tacacgtcca aaattattta
     3481 cactcggatt aaaactcatc ttggcccaga aaacccactc catgctatcg gcaagtgtaa
     3541 cattgaaggg agttgacaga tatgttgtat cagctactgt attcgtcgat tgcaaaccag
     3601 gatgcattga agatggaatc gccgttgaag acgaaatgat ataattggcc gcatcgccgc
     3661 tccatacggg atttgaagta aaattaccgt cggaaaaatc atcactcatc tgggcatgtg
     3721 cagctgcaga gagaaacatc agaaataaaa atatccagcg cataataatg aattatctgg
     3781 taaagatagg aataaaaatg agacgatgat tcgaaatttg aaatttcagc tttctttaag
     3841 gagtaggata aatgatacgg gaaaactgaa ggatttggaa ttgtcatcat tctattcaag
     3901 ttttatcctg tttttatacc attgtgaaat atatcttatg ctgatgtgtt tatgcgaaaa
     3961 ttgatagccg atacctgaca aacaggcagg catcgcatgt ctgtagatat tgatcaaaca
     4021 atagtacgac ctcagagagg ttgaatgtaa atggctgcct gtgcgaaaca gcttgaaaaa
     4081 cattcgactc cgctggagtc
//
LOCUS       DAOPKE010000159         4055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_24369_length_4055_cov_10.894250, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000159 DAOPKE010000000
VERSION     DAOPKE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24369_length_4055_cov_10.894250"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKY63_12435"
     CDS             <1..690
                     /locus_tag="PKY63_12435"
                     /inference="COORDINATES: protein motif:HMM:NF023607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPF01466.1"
                     /translation="ARIDASRTLLYETSRYVDVYKGYAHKALHHTLTADERNEMKHYN
                     RWSDVMTPLTKLFATEYSNMVAYDAIQVLGGSGYMKDYPVERYYRDARITNIYEGTSQ
                     LQVVAAIKGVINGTFQNWMEGRAKVKVKSEYQYLIDILAKMQDQFVKTVDKVKEINHA
                     ELLDFHARRLVEMAGHIIAGYLLVHDANMDDMFAKSADIYVRHGRSHNIAAAHYIMHT
                     DQEDLSNYSSN"
     gene            1299..2009
                     /locus_tag="PKY63_12440"
     CDS             1299..2009
                     /locus_tag="PKY63_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01467.1"
                     /translation="MINRLFVSLFLTALICCGVSEVIVAQKNNTETLHWWIEGGINNS
                     FKEAYYPATDFSQPLSNANCYVGLPFSIETRSLTSGQISGGFSFPVNKFVNLNAGIEM
                     VSIRDEIITDRDSVRHNMDSLFSFFIEKNNTIYFPLSLGASAEFRRMNIEARFAFPLL
                     NINHKNGFDKSSERSISNSTAVWNMHFPAIIIRGKLGYHLSEKVELFCMYSRNFSKEI
                     AYDNHYSLGIQYSFIQNK"
     gene            2012..2716
                     /locus_tag="PKY63_12445"
     CDS             2012..2716
                     /locus_tag="PKY63_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01468.1"
                     /translation="MKQSILIIALIIIFNLPKFAFAQRDSSATGINIKVNPFQWYYDD
                     IRVFAEYPLSKNNSLQLRLGYRDYRINPYDQKYNDRDQIYRDPVTDQYDTALYSAVGF
                     SLGLAYNHYDRNNWYIQPSFLFRHTSFSFGWDDNWNNWVLQWLTYDTTSEGADISDFI
                     KNIYALEFRYGKVFIFNRFILDLFTGAGVRLRYGRIIPGSGYGITHDQVFYHSTEYEV
                     TPTLHLGLNIGWIIKK"
     gene            complement(3023..>4055)
                     /locus_tag="PKY63_12450"
     CDS             complement(3023..>4055)
                     /locus_tag="PKY63_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPF01469.1"
                     /translation="NKMVYYISFEDGPMNKMLYSVSFDGVKKKITSTLGSHSVTFSSD
                     FAYYIDNWSDANTAPKYSICNNKGKEQLVLEDNADLNKKMEEYGFSKEEFFSFKTSEN
                     VELNGWMIKPANMKKGKKYPVFMYMYGGPGVNTVENSFGYFDFAWFQMLAQKGYIVVS
                     VDNRGTGGRGEEFKKCTYMQLGKHEVADQIEAAKYLGTLDYVDKSRIGVFGWSFGGYL
                     STLCMTKGNEYFKTGIAVAPVTNWRYYDNIYTERFMRTPQENAEGYDSNSPINFVKKL
                     KGKYLLVHGTTDDNVHVQNSMDLVSALVNANREFDMFFYPNKNHGIYGGYTRYHLYNK
                     MTNFILENL"
BASE COUNT         1158 a          908 c          827 g         1162 t
ORIGIN      
        1 gcacgcatcg atgcctcacg aacattgctg tatgaaacaa gccgctatgt ggatgtttac
       61 aaaggatatg ctcacaaagc gctccatcac acacttacag ccgacgagcg caacgaaatg
      121 aaacattaca atcgctggtc cgatgtcatg acaccgctca ccaagctgtt tgcaaccgaa
      181 tacagcaaca tggtagccta cgatgccatt caggttttag gcggatcggg atacatgaaa
      241 gactatccgg ttgagcgcta ctaccgcgat gcacgcatca ccaacattta cgaaggaact
      301 tcgcaattgc aggttgttgc cgccatcaaa ggtgttatca atggtacttt ccagaactgg
      361 atggaaggac gcgcaaaagt gaaagtgaaa tccgaatacc agtatctgat tgatattctt
      421 gcaaagatgc aggatcagtt tgtaaagact gttgataagg taaaagaaat caatcatgcg
      481 gaactgcttg atttccatgc acgtcgtctg gtggaaatgg ccggtcacat cattgccgga
      541 tatctgctgg tacacgatgc caacatggat gatatgtttg cgaaatcagc agatatttat
      601 gtacgccacg gccgctcgca caacattgca gcagcgcatt acatcatgca caccgatcag
      661 gaagacctga gcaactacag cagcaactaa ttctgattaa catatgaaag gccggacttg
      721 ttccggcttt tttattgtgc cacgaaggct caaagtcaca aagcaaggag cgcaaggcct
      781 tctccaacct ctctgcgtcg ctgaaagcga cctgagtgcg ttggagaact gtcatggcgc
      841 agatatgcca tccttcacat ggctgcctgc agcaaaaaac aactctccct gacgattcca
      901 cgtcagcaat gtctgtttgt tatccgcaac aagctgacgg tagaatgtca ggggatgcat
      961 ttgctgtcat cctgagactc gctcctgtag cacttttaca gaaatagacc tgcgatgtat
     1021 ccctgtccac agcgcagctg gtggagaagg atagacagct gagcccgctg tcgttcttcc
     1081 ctgcccgctg tgccgcaggc ggtgatactt ttgaagcttc ttcaacaaaa caaagtctct
     1141 ggttcaaaaa ctttgaatga cagcgggaag acagcgaccg ggatcttgat tctacttcca
     1201 ttgattttca aatcaaacta aatcaaaaca atttttatca gaaattgtcg ttacatttga
     1261 ttgtttgatg tgctgaatta attatttaat attgtattat gatcaaccgg ttatttgttt
     1321 cattatttct tacagccctg atatgctgcg gtgtttcaga ggttattgtt gcgcagaaaa
     1381 acaatactga aacacttcat tggtggattg aaggtgggat caataactca ttcaaagagg
     1441 catattatcc ggccaccgat ttcagtcagc ctttatccaa cgccaattgc tatgtaggat
     1501 taccattcag catagaaacc cgctcattaa cgtcaggaca gattagcggt ggattttcat
     1561 ttccggtaaa caagtttgtg aatctgaatg ccggaataga aatggtttct attcgtgacg
     1621 aaataattac agacagagac agcgtgcgcc acaacatgga ttccttgttc tcatttttca
     1681 ttgaaaaaaa caatacaatt tattttcctc tgtctttggg agcaagcgct gaattcagga
     1741 gaatgaatat cgaagcgcgg tttgcatttc cactgctgaa tatcaatcat aaaaacggat
     1801 ttgataaaag ctcggaacgc tcaatatcca attcaactgc tgtctggaat atgcatttcc
     1861 ctgcaatcat tatacgcgga aaactgggat atcacctatc tgaaaaagtt gaattattct
     1921 gcatgtattc cagaaacttt tcaaaagaaa ttgcatacga taatcattat tcattgggca
     1981 tccaatattc gtttattcaa aataaataat catgaaacaa tcaatactga tcattgcatt
     2041 aataatcatc ttcaacttgc caaagttcgc attcgcgcag cgggacagct ctgcaaccgg
     2101 aatcaatatc aaagtcaatc cctttcagtg gtattacgat gatatacgtg tatttgcaga
     2161 gtatccgctc agtaaaaaca attcgcttca attgcggctt ggttacaggg attacaggat
     2221 taatccatat gatcagaaat acaatgaccg cgatcagatt taccgcgatc cggtaacgga
     2281 tcaatatgac acggccttgt attctgctgt gggattttca ctgggactgg catataatca
     2341 ttacgacaga aacaactggt acatacagcc atcctttcta ttccgccaca catctttctc
     2401 ttttggttgg gatgataact ggaataactg ggtattacaa tggttgacct acgatacaac
     2461 aagtgaaggt gcagatatca gtgattttat caaaaatatt tacgcgcttg aattcagata
     2521 cggaaaagta tttattttca accggtttat tcttgatctg tttaccggag caggagtcag
     2581 actgcgctat ggcaggataa taccaggctc cggctatgga attacgcacg atcaggtttt
     2641 ttatcacagt actgaatatg aagtaacccc aaccctgcac ctggggctga atatcgggtg
     2701 gattatcaaa aaataatgct gcgtttttgt gatcgcactt cttaaaagcg gagaaaatca
     2761 acaaaaaaag tctgcagtat aaaaagagat cccggctctg aggccgggaa cttcattaaa
     2821 cttttttgtt gattttttaa aatccgtata aatcaaactt cagctcaaaa atcaaaaatc
     2881 aaaaatcctt gcctgcctgg ctggctgttg cagttaaacc ggcagacagg ttcttaaatc
     2941 agaacttcct tcttacttca cacttccttg ttccttcaca catccttctt acttcacact
     3001 tccttgttca gtgttcaata ttctacagat tttcaagaat aaaattcgtc atcttattgt
     3061 acagatgata cctcgtatat ccgccataga tgccgtgatt cttgttcgga tagaaaaaca
     3121 tatcgaactc gcggttggca ttcaccagtg cggaaaccag atccatgctg ttctgcacat
     3181 gcacgttgtc gtcggtggtg ccgtgcacaa gcaaatattt tcctttcagt ttcttcacaa
     3241 aattgatggg tgaattgctg tcgtaccctt cggcattttc ctgcggagtg cgcatgaaac
     3301 gctcggtgta gatgttgtcg tagtagcgcc agttggtcac cggagcaaca gcaattcctg
     3361 ttttaaaata ttcatttcct ttggtcatac agagtgttga aagatatccg ccgaaactcc
     3421 agccgaacac gccaatgcgg ctcttgtcaa cgtaatcgag tgtgcccaga tattttgctg
     3481 cttcaatctg atcagccact tcatgcttcc caagctgcat gtatgtgcat tttttaaact
     3541 cctcgccacg accaccggtg ccgcggttgt cgacggagac aacgatgtag cccttttgtg
     3601 ccagcatctg aaaccaagca aaatcgaaat aaccaaaact gttttcgaca gtattcacgc
     3661 cggggccacc atacatgtac ataaacaccg gatatttttt cccttttttc atgttggccg
     3721 gcttgatcat ccagccgttc agttctacgt tttcagaggt tttgaaagag aaaaattctt
     3781 ctttcgaaaa accatattcc tccatttttt tattcaggtc agcgttgtcc tcgagcacca
     3841 gctgctcttt tcctttattg ttgcagatgg aatatttcgg ggcagtattg gcatcggacc
     3901 aattatcgat gtagtatgca aagtcgctgc tgaaggttac gctgtgtgat ccaagcgtcg
     3961 atgtgatttt ctttttcaca ccatcaaacg aaactgaata cagcatttta ttcatcggtc
     4021 cgtcttcgaa cgaaatgtaa tacaccattt tattt
//
LOCUS       DAOPKE010000160         4037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_24513_length_4037_cov_13.798343, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000160 DAOPKE010000000
VERSION     DAOPKE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24513_length_4037_cov_13.798343"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKY63_12455"
     CDS             <1..387
                     /locus_tag="PKY63_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPF01470.1"
                     /translation="QKEINYLNNYISLHELKFGPDFIRFNNDLKNTNEIVAPMLFIPL
                     VENAIKHGNKKVASPGITIDLYEKNDVLCFVVRNYISRHEVNKDAAGGIGMVNLKRRL
                     ELLYPQRHSFSTEIENGIFTAKICLL"
     gene            384..1127
                     /locus_tag="PKY63_12460"
     CDS             384..1127
                     /locus_tag="PKY63_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPF01471.1"
                     /translation="MKLKCIVVDDEPLAVEKMSAYIARVPFLELQASFTSGMDALQYL
                     KDNSTDLLFLDIQMDDLTGIQLLEVLKNKPQVVFTTAYSDYALKGYELEVSDYLLKPI
                     SFERFLQSANRAYDKMSDKTKSEVKNEQLAANNPPSPDFMFVKTEYRMEKVKFNDIHY
                     VEGMKDYLRIVCPEKRIMTLTNFKNMLDMLPEEAFCRIHKSYIINLSKIQSIEKSHVV
                     VLNERLPIGDSFRKSFFELLEKLRLINNK"
     gene            1213..3426
                     /locus_tag="PKY63_12465"
     CDS             1213..3426
                     /locus_tag="PKY63_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPF01472.1"
                     /translation="MKRLITILALVGLMFKGAYAVAPPDEGMWLVMFIGKYNYEEMKR
                     LGLKLTAEQLYSINNASVKDAIVGLGNTGMAPEGYFCTGEIVSDKGLVLTNHHCGYDA
                     IQTHSTLEHDYLTNGFWAKSMDQELPVKDMTMSFLVRMDDVTAQVLEKVTDDMSDDDR
                     QSAIDEASAKIAKEASEKGKYDATVKSMFDNNEFYLFVYETYSDIRLVGAPPSSIGKF
                     GGDTDNWMWPRHTGDFSMFRIYTSPEGKPATYNEKNIPLKPKHYLPVSIKGVQKDDFS
                     MIFGFPGTTNRYLTSHGVDVQLKQSNPAVVKIRDKKLEILRGYMDADPKIKIQYASKY
                     AQTANYWKYFIGQSKGLVRWDVYNEKKAIEDEFQKWVNADPSREKKYGKCLSDIDAGY
                     KDYVKYNLSMQYLNEAVFQGPDYIYFSFGAFQLYGALKQQADADKNEKAKYDGVIKNQ
                     AAAFEEKLADFYKDYNKQVDKDQFIALLEMYYNDIPEEQHPSIFKTVKGKKFKGSFKA
                     WGEYVYANSIFVDEAKLRAFLKAPSYKVISADPGFAVTLSMVDAIRSLYGDLSVVEEK
                     INNGSRLFVDGLRKMNPSRKYYPDANSTLRMTYGTIQDYYPIDAVHYDFRTTLDGVME
                     KEDSTKDEFVVEKRLKELWAAKDFGPYAENGELYVCFTSNNDITGGNSGSPVINGNGE
                     LIGIAFDGNWESMSGDIKYEASVQRTISVDIRYVLFVIDKYAGATNLIEEMKIVK"
     gene            3842..4015
                     /locus_tag="PKY63_12470"
     CDS             3842..4015
                     /locus_tag="PKY63_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01473.1"
                     /translation="MKHVCFTVESKWMSTANNEKVVLSNVVRRDIFLAEEEKDPTMLP
                     WQFGCQRQMDGDH"
BASE COUNT         1265 a          735 c          843 g         1194 t
ORIGIN      
        1 cagaaagaaa tcaattatct gaacaattat atttcattgc atgaattgaa gtttggaccc
       61 gattttatca gattcaacaa tgatttgaaa aacaccaatg agattgttgc gcccatgttg
      121 tttattccat tggtcgaaaa tgccatcaag catggaaata aaaaggttgc ttcgcccgga
      181 ataaccattg acttgtatga aaagaatgat gtcctttgtt ttgtggttag aaattatata
      241 agccggcacg aagtgaacaa ggatgctgca ggcggaatcg gcatggttaa tctcaaacga
      301 cgtctggaac ttctttatcc gcaacgtcac tcattttcaa cagaaattga aaacggaata
      361 tttactgcaa aaatctgtct gttatgaaac tcaaatgcat tgtagttgat gatgaaccgc
      421 tcgctgtaga aaaaatgagc gcttatattg cacgtgttcc ttttcttgag ttgcaggcat
      481 cttttaccag tggcatggat gctttgcagt atcttaagga taactcaaca gacttattgt
      541 ttctcgatat tcagatggat gatcttacgg gaattcagtt gctcgaagtg ttgaaaaaca
      601 aacctcaggt ggtttttaca acagcataca gtgactacgc tttgaagggt tatgaacttg
      661 aggtaagtga ttatctgctg aaacccattt cgtttgaacg ttttctgcaa tcggccaaca
      721 gggcttatga taaaatgtcg gataaaacga aaagtgaagt caaaaacgaa cagctggctg
      781 caaacaaccc gccgtcaccg gattttatgt ttgtgaaaac agaatatcgt atggaaaaag
      841 tgaagttcaa cgatattcat tatgtagaag gcatgaagga ttatctgcgt attgtttgcc
      901 ctgaaaaacg gattatgact ttgaccaatt tcaaaaatat gcttgatatg cttcccgaag
      961 aagcattctg ccgtatccac aaatcataca tcatcaatct ttcaaaaatt caaagcattg
     1021 aaaaaagtca cgttgttgtg ctcaacgagc ggcttccgat aggcgacagt tttcgaaagt
     1081 ctttttttga attgctcgaa aaactgcgat taatcaacaa caaataacct gaaaaccttt
     1141 ttcgtacggt tgaattgcga aaaatgaata tttttgtcat cccgaattta aactgaaaaa
     1201 caacaataac aaatgaaacg actgattaca attctggccc tggtgggtct gatgtttaag
     1261 ggagcttacg ctgtagctcc acccgatgag ggaatgtggc tggtgatgtt catcggcaaa
     1321 tacaattatg aagaaatgaa acgccttggt ttgaaactga cggctgagca gctttacagt
     1381 atcaacaatg ctagtgtcaa agatgcaatc gttggtcttg gaaacacagg aatggctcct
     1441 gaaggatatt tctgtactgg cgaaatcgtt tctgataaag gtctggtgct taccaatcac
     1501 cattgcggct atgatgccat tcagacacac agtacactgg aacacgatta tctcaccaat
     1561 ggattctggg ccaaaagcat ggatcaggaa ttgccggtga aagatatgac catgtctttt
     1621 ctcgttcgta tggatgatgt tacggctcag gtgcttgaaa aagttactga tgatatgagc
     1681 gatgacgatc gtcagagtgc tattgatgag gccagtgcta aaattgcaaa ggaagcttct
     1741 gaaaaaggta aatacgatgc aacagtaaaa agcatgttcg acaacaatga gttttatctc
     1801 tttgtttatg aaacatacag cgatatccgc ctggttggcg ctccgccttc aagtatcgga
     1861 aaatttggtg gcgacaccga caactggatg tggcctcgtc ataccggcga cttcagtatg
     1921 ttcagaattt atacttcgcc cgaaggtaaa cctgctacct acaatgaaaa gaatattccg
     1981 ctcaaaccaa aacattattt gcctgtaagc attaaaggtg ttcagaagga tgatttttca
     2041 atgatttttg gttttcccgg aacgacaaat cgctatctta cttctcatgg ggtcgatgtt
     2101 cagcttaaac aaagcaaccc ggccgtggtg aaaatccgtg ataaaaaact tgaaattctt
     2161 cgcggttata tggatgctga tccaaaaatc aaaatccagt atgcttcaaa atatgctcaa
     2221 acagccaatt actggaagta ttttattggt cagagcaaag ggcttgtgcg ttgggatgtt
     2281 tacaatgaaa agaaagccat cgaagatgaa tttcagaaat gggtcaatgc tgatccttcc
     2341 cgtgagaaaa aatatggaaa atgtctttcc gatatcgacg ccggatacaa ggattatgtg
     2401 aaatacaacc tttctatgca gtatctcaac gaagctgtat ttcagggtcc cgattatatt
     2461 tatttttctt ttggtgcatt tcagctctat ggtgctttga aacaacaagc cgatgccgac
     2521 aagaatgaaa aggcaaaata tgatggtgtt attaaaaatc aggcagctgc ttttgaagaa
     2581 aaactggccg atttttataa ggattacaac aaacaggttg ataaagacca gtttattgct
     2641 ttgctcgaaa tgtattacaa tgatattcca gaagaacagc acccttccat ttttaaaaca
     2701 gtgaaaggaa aaaaattcaa aggtagtttt aaggcctggg gtgaatatgt ctatgcaaac
     2761 agtatttttg ttgatgaagc aaaattacgc gctttcctca aagctccgtc ttataaggtg
     2821 atttcggctg accctggttt tgctgtaact ctcagcatgg tcgatgctat ccgttctttg
     2881 tatggcgatc tttctgttgt ggaagaaaaa atcaataatg gttcccgctt gtttgttgat
     2941 ggattgcgta agatgaatcc ttcccgcaag tactatcccg acgccaatag cacgcttcgt
     3001 atgacttacg gaaccattca ggattattat cccatcgacg ctgttcatta tgatttccga
     3061 acaacccttg atggtgttat ggaaaaagaa gattctacaa aagatgaatt tgtcgttgaa
     3121 aaacgattaa aagaattgtg ggctgccaaa gattttggtc cttatgccga aaatggtgaa
     3181 ttgtatgttt gtttcacttc aaacaatgat attaccggtg gtaattccgg aagtcctgtg
     3241 attaacggaa acggcgaact gattggaatt gctttcgacg gaaactggga aagcatgagc
     3301 ggcgacatta agtatgaagc ttctgttcaa cgtactattt ctgttgatat ccgatatgta
     3361 ctttttgtca ttgacaagta tgccggtgca actaatctta tcgaagaaat gaagattgtg
     3421 aaataatttt cgcagccatt tttcaaaaaa gccggaagtg attccggctt tttttgtggg
     3481 ttttttcatc gtaaaacaaa acctgcaatg atattttata ccaaagtgat ttatgaatca
     3541 gtatagtcat ttttggaaga ctccggagga gtcacatatc tgtagaaaat ctaattataa
     3601 aacacgactc cagcggagtc gaatgttttt caagctgttt cgcgcaagaa gccgtttaca
     3661 ttcgacctct ctgaggtcgt tgtgtgtata tgcgcttttc tacagacatg cgatgcctct
     3721 ggcatcagcc atcaattttt gctaaaacaa acagacctaa aatatatttc acgatggtat
     3781 tagctccatc cgtgatccat gaaggaaaat tttccattga aatatttcat aactttacgc
     3841 tatgaagcat gtctgtttca cagttgaatc aaaatggatg tcaacagcaa ataatgaaaa
     3901 agtggtatta tcaaatgtgg tcagacgaga tatattcctt gcagaagaag aaaaagatcc
     3961 tactatgtta ccatggcaat ttggatgcca acgtcaaatg gatggcgacc attgattaat
     4021 tttaactact ataatta
//
LOCUS       DAOPKE010000161         4036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_24522_length_4036_cov_13.249184, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000161 DAOPKE010000000
VERSION     DAOPKE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24522_length_4036_cov_13.249184"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..2624
                     /locus_tag="PKY63_12475"
     CDS             30..2624
                     /locus_tag="PKY63_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF01474.1"
                     /translation="MRKSHLLILTLLLIAASACSTKKNTAIRRGYHNLTSHFNAYFNG
                     NEALKEGVMNLSVAHIDNYTQIISVFPEGTDKDVMGITPQMDRAIEKAYKVIAKHSME
                     FKGVEYVKWIDDSYLMIGKASFYKHDYEKASSTLDYVTKKYNKNPERFDAYLWLARTM
                     IAKNKLSKTSSYLSIVEIAQNEGSLLKRTKKMLPLVQADYNIRSANYETAIENLKKAI
                     ALNKTKKTKVRLMFVLAQVYQRLERNSEALAMYQQVLKKNPKYEIAFHARIFAAQCYD
                     AGSGSSDAIVKELEKMLKDSKNDDYRDEIYFALANIAFKDNKEQQGIDYLLLSAKYST
                     VNFYQKATTYLKLGQLYYDKRDYKKSQVFYDTCMTVLPKDYPGYDKIKSRHAVLNELV
                     FNLTTIETEDSLQRLANMSEANRNAAIDKLIQEYIKEEERKKREERERMAAAQNSNLP
                     NVANANSNWYFYNAQTVAFGKTEFRKKWGERELAPLWRLENKQVIDFGFEENGTDTVA
                     LDDSAIAATNPRDRSYYMKDIPLTPERIEASNDKIEKAYFDLGRIYKTELEEFNDAVK
                     AHETLLSRFDSTNYKLETYYMLYSSNKSLKNEQRADFYKNLILSKFPDSEFAKILSDP
                     DYWLKVAAQKNVGEQYYEDTYLTYQSGNHQKVIQRCDSALNVFKEVSLLGRFDFLKAV
                     SLGKIYGNDTLKARMNKISAHYTGDIKQRADDILAYLGGNTNVETTDNNTTTATEVPK
                     ELYKPSDNEVIHIFVCLIDAKTTNLNKLKAAFSDFNGTYFGSENLSVSSLYLTDSRMM
                     ISVSHFKSKTTGMTYLRFTKNDKNLTDLILSSNPVTFIISTENYPVFYKSKDEMKYLE
                     FFNRYYVE"
     gene            2645..3010
                     /locus_tag="PKY63_12480"
     CDS             2645..3010
                     /locus_tag="PKY63_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPF01475.1"
                     /translation="MAKTITSEPVTPTINIIATGTVIKGDIKANGDIRVDGQVVGNLI
                     VQGRIVVGGSGQVEGEIHCQNGDFSGEVKGKVFVSELLTIRSTASINGDIQTQKISIE
                     PGAVFTGNCSMQEQQPEIQ"
     gene            3017..3241
                     /locus_tag="PKY63_12485"
     CDS             3017..3241
                     /locus_tag="PKY63_12485"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPF01476.1"
                     /translation="MTKDKEDLKKKGNDFARFSGMAFEMLVIIGGMTWGAVWLDKQTG
                     TEIPWFTIILSPLSVIIAVLMIIKDLNRNK"
     gene            3241..3618
                     /locus_tag="PKY63_12490"
     CDS             3241..3618
                     /locus_tag="PKY63_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01477.1"
                     /translation="MNQLIWRPFFIFLSIINILVWSIAAIFPELYGISWPWSLGLLAV
                     LNALSFLVFQRSLKQDSKASALIFLALTTGKMLLGMIYILVLVMGFGIDSLSDSLFFV
                     ILYLLFLALEVVVFTKNVKQDKR"
BASE COUNT         1332 a          844 c          835 g         1025 t
ORIGIN      
        1 tttattcgtt atcagaccaa aataccggca tgaggaaaag ccatttattg atactaacac
       61 tgcttctgat cgcagcttcg gcgtgttcga ccaagaaaaa cacggccatt cggcgcggtt
      121 atcataatct cacatcgcat ttcaacgcct atttcaacgg caacgaagcc ctgaaagaag
      181 gggttatgaa tctttcggtt gcacatattg acaactacac acagattatt tccgtttttc
      241 cggaaggaac cgataaggat gtaatgggga ttacgcctca gatggaccga gccatcgaaa
      301 aggcctataa ggtgattgcc aaacacagca tggaattcaa gggcgttgaa tatgtgaaat
      361 ggattgacga cagctatctg atgattggca aagccagttt ttacaaacac gactatgaaa
      421 aggccagcag cacgctggat tatgttacca agaaatacaa taaaaatcct gaacgtttcg
      481 atgcctattt gtggctggcc agaacaatga ttgcaaaaaa caagctgagc aaaaccagtt
      541 cctatttatc aattgttgaa attgcacaaa acgaaggcag tttacttaag cggacaaaaa
      601 aaatgttacc attggttcag gccgattaca acattcgttc agcaaactat gaaaccgcca
      661 ttgaaaatct gaaaaaagcc attgccctca acaaaacaaa gaaaacaaaa gtacgtctga
      721 tgttcgtatt ggcgcaggtt tatcaacgtc ttgagagaaa cagcgaagcg ctggccatgt
      781 atcagcaggt gttaaaaaag aatccgaaat atgagattgc ctttcatgcg cgaatatttg
      841 cggcccaatg ctacgatgcc ggaagtggaa gcagtgatgc cattgtcaag gaactcgaaa
      901 aaatgctgaa agacagtaaa aatgacgatt acagagatga gatttatttc gcactggcaa
      961 acatcgcttt caaagacaat aaagaacaac aagggataga ctacctgctg ctttcggcca
     1021 aatacagcac ggtaaatttt tatcaaaaag ccacaaccta cctcaagctt ggtcaattat
     1081 attacgacaa acgcgattac aaaaaatcac aggtattcta tgatacatgt atgactgttt
     1141 tacccaaaga ttatccagga tacgacaaaa tcaaaagccg ccatgccgta ttaaacgagc
     1201 ttgtgtttaa tctcacaacc atcgaaaccg aagactcatt gcagcgactg gccaacatgt
     1261 cggaagccaa ccgaaatgca gccatcgaca aacttattca ggaatacatc aaggaagaag
     1321 aacgcaaaaa acgtgaagaa cgtgaacgca tggccgctgc gcaaaacagc aacctgccaa
     1381 acgttgccaa cgcaaactca aactggtatt tttacaatgc acaaacagtt gctttcggaa
     1441 aaacagagtt cagaaaaaaa tggggtgaac gcgaactggc gccactctgg cgattggaaa
     1501 acaagcaggt tatagacttt ggttttgaag aaaacggtac tgataccgtt gcattggacg
     1561 actctgctat agccgcaaca aatccgcgcg acagaagtta ctacatgaag gatattccac
     1621 taacccctga aagaattgag gcgtcgaatg ataaaattga aaaagcatat ttcgatcttg
     1681 gaagaattta caaaacagaa ctggaagaat tcaatgacgc tgtaaaagca catgaaacgc
     1741 tgctttcaag atttgactcc accaactaca agctcgaaac atattatatg ctttattctt
     1801 caaacaaaag tctgaaaaat gagcaaagag ctgactttta taaaaacctg attctttcaa
     1861 agtttcctga cagcgaattt gcaaaaatcc tttctgatcc ggattactgg ctcaaagtgg
     1921 cagctcaaaa aaatgtcggc gaacaatatt acgaagacac ttatctgaca tatcagtcag
     1981 ggaatcatca gaaagttatt caacgctgcg attcagccct gaatgtgttt aaagaagttt
     2041 cactccttgg caggttcgat tttttaaagg cggtgagtct tggaaaaatc tatggaaacg
     2101 acaccctgaa ggcacgaatg aataaaatat cagcacatta taccggcgac atcaagcagc
     2161 gggccgatga tattctcgcc tacctcggcg ggaataccaa tgttgaaaca acggataata
     2221 atactacaac tgcaacggaa gtcccaaaag agctctacaa gccatcggac aatgaggtca
     2281 ttcacatttt tgtatgtctt attgatgcaa aaacaacaaa tttaaacaaa cttaaagcgg
     2341 cattttcaga cttcaatgga acatatttcg gctcggaaaa cctaagtgta agcagtttgt
     2401 atttgactga cagccgcatg atgatttcag taagtcattt caaatccaaa accaccggaa
     2461 tgacttatct tcgttttacg aaaaatgata aaaatctgac agatttaatt ctcagcagca
     2521 atccggtcac cttcattatc agcaccgaga attatccggt gttttacaaa tcaaaagacg
     2581 aaatgaaata tctggaattc ttcaacagat actacgttga atagcaaaac agaaaggaaa
     2641 taatatggcg aaaacaatta caagcgaacc ggtaacacca accatcaaca ttattgcaac
     2701 cggaacagta attaaaggtg acatcaaagc aaacggcgac attcgcgtag atgggcaggt
     2761 tgttggaaat cttattgttc agggacgaat tgttgttgga ggttctgggc aggtggaagg
     2821 agaaattcac tgtcagaacg gcgatttcag cggcgaagtg aaaggaaagg tttttgtttc
     2881 tgaattgctg accatacgtt ctaccgcttc tatcaacgga gacattcaaa ctcagaaaat
     2941 cagcatcgaa ccgggcgctg tgtttaccgg aaattgcagc atgcaggaac aacaaccgga
     3001 aattcaataa tttttaatga ccaaagacaa agaagacctg aagaaaaagg gcaacgactt
     3061 cgcccggttt tcgggcatgg ctttcgaaat gctggtgatt atcggcggaa tgacctgggg
     3121 cgccgtatgg ctcgacaaac agaccggaac cgaaatccca tggtttacaa ttattctttc
     3181 ccccctatcc gtaattattg ccgttttgat gattataaag gatttaaacc gaaacaaata
     3241 atgaatcagc tgatctggag accatttttc attttcctta gtattattaa tatattggtc
     3301 tggagtattg cggccatttt tccggaatta tacggaattt cctggccatg gtctttggga
     3361 ttgctggctg tactcaatgc cttgtcattc ctcgtattcc agagatcgct gaagcaggac
     3421 tccaaagcct ccgcactcat atttctggca ctcaccaccg gtaaaatgct actgggaatg
     3481 atttacatcc tcgtcctcgt gatgggattt ggcattgact cgctcagcga ctctttgttt
     3541 tttgtgattt tgtatctgct gtttctggca ttggaggtgg tggtttttac aaaaaatgtg
     3601 aagcaagata aaagataata gctctgcact gatcctgtcg aagtggaaaa agacaaaagt
     3661 ttataagggc tggtgaatag tcgggatcat ctataaagcc tttaaagctt ctccactttt
     3721 tcttccacga attttacgaa ttacacgaat gaagacacca gcgtcgcaac ctctgatttt
     3781 agcgaataaa ttaccagtca tctgcgatga aaggtgattt cacgaattgg atttcgtacg
     3841 tcacgccacc tttccacttt aagaacctga tgatcattca actactaagt tcccggccgg
     3901 agctgaaagc agcatgtgaa tagttcaaag cttgtgaaga gccgggatcc cctcaataac
     3961 cttctacttg tcacagcttg ccgggtttta cgcggttcta cgtgaagcca ttgctgaatc
     4021 aatgattgat ttactc
//
LOCUS       DAOPKE010000162         3949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_25256_length_3949_cov_12.994093, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000162 DAOPKE010000000
VERSION     DAOPKE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25256_length_3949_cov_12.994093"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1888
                     /locus_tag="PKY63_12495"
     CDS             350..1888
                     /locus_tag="PKY63_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF01478.1"
                     /translation="MSKKRFSVHAGIFHGRTAPEQGVIVGYAAIIDTLGLEIPMPSIL
                     SLICTTRKQYSTGEWSVFSSRAAFDDTLYRHLVFALKYEGLNLLFFKKLFAKLSTKEI
                     LGVLEEEPTGQYSRKIWFLYEWQMNKELPIDDLAIKNYVPLVDDSLQFALYTGSKSPR
                     HRIINNLPGTRDFCPLIRKTAKLDNYIKENISGHNSDYLKKIHKDILQRTSAFLLLKD
                     SKASFSIEGESPKSSRAARWGRIIGQAGNNELTKEELIRLQQAVIENARFVKLGFRKQ
                     GGFVGEHDRVSGEPVPDHISAKWQDVEQLIDGLIASNDLLLKNEMDAVLTAAVIAFGF
                     VFIHPFVDGNGRIHRYLIHHVLMKKQFSAQGLVFPVSASILNHIDDYRKALESYSLPL
                     LDFIEWKETSDHNVEVLNDTIDYYRYFDATKLAEFLYDCVNDTIQNIIPAELKYIQQY
                     DEFKRYIENRFEMPDKLIATLVRFLEQNKGKLSQRARKKEFEALTATEIREIEKSFDD
                     IFGL"
     gene            complement(2062..3600)
                     /locus_tag="PKY63_12500"
     CDS             complement(2062..3600)
                     /locus_tag="PKY63_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPF01479.1"
                     /translation="MKLQEIIKLPEGRRLEFKQKLPASADLTKTIVAFANDAGGDVYI
                     GVQNNPRKLTGIPEDELLKTEEKIASIIHDNCCPVIVPDITFPVENGCYLIRVQIHRG
                     SDFPYHLKSKGIHGGTYIRIGSTNRLADDSIIEELQRRKRNISFDSEPLHGNAISDFD
                     LSSFEKQFKELSGENLTQATLKKLLLIKESRGKQLPTNALALFADYESRKDLFPYSKV
                     ECARFKGVSTETKMDDKTITDQIGLQPAEALKFVERHVDKESVIDGAYTKSRWAFPMK
                     AIREVLRNAVAHRDYSLTGKDIKVAVYDDMVEILSPGTLLPSINFDDLEARQSDIRNK
                     VIAPVFKHLGIIDQWGNGLKIISQELKLYPEIEFKWFETGLQFQVQFIKKDNQPAIQQ
                     NSVFDSIGREIAVIFGLSWDQAGTRSEPGRDQAGTKLALSWHQVENLLVFSQTSRSVK
                     EFMTLLNWKSRTKFRAKYITPLLGMGLLQMTVPNKPSSPNQQYFLTQKGHGFLEFLKK
                     SLKM"
BASE COUNT         1138 a          834 c          771 g         1206 t
ORIGIN      
        1 attttcggaa agtattctgg gaagtgaatt ggattttttg ctgatgtaac ctcctgaaat
       61 cgcaatgctt tacttgaaac aatctctgcc atgcctttcg cgacattagc ctgacccact
      121 tgaaaatctc ttttcaggcc gtcatatttt gcttttttca aaattctaag cctgctctac
      181 ctgtttttcc atgggaatac cgatttcgcc attttcctga attattatac ctgacattcc
      241 attgtattca taattattac ctgcttttcc acaattattc atttttttac ccgtttttcc
      301 cacattttac ctaaaaatcc ctatatttgc aaaattcaaa tcttgcacca tgagtaaaaa
      361 gaggttttct gtacatgcag gcatatttca cggccggaca gcaccggagc aaggggtaat
      421 agtaggctat gccgcaataa tagataccct tggtctggaa ataccgatgc cttcgatatt
      481 atcgctgata tgcaccacga gaaaacaata cagcaccgga gaatggagcg ttttttcttc
      541 gcgtgcagcc ttcgacgaca cattataccg gcaccttgtg tttgccctta aatacgaagg
      601 actaaatctg ttgttcttca aaaaactctt tgcgaaactc agcaccaaag agatattagg
      661 tgttcttgaa gaggaaccga cggggcaata ctcaagaaaa atatggtttc tatatgaatg
      721 gcagatgaac aaagagcttc ccatcgatga tctggcgata aaaaactacg tgcctcttgt
      781 tgacgattcg ctccagtttg ccttatatac tggctcaaaa tcgccaagac acagaatcat
      841 caacaatctg ccggggacca gggatttctg ccctttgatc cggaaaacag caaaactgga
      901 caattatata aaagaaaata tttctggtca caattcagac tatctgaaaa aaattcacaa
      961 agatattctt caacgaacct ccgcattttt acttctcaag gattccaaag cgtcattcag
     1021 tattgaaggt gaaagcccaa aaagcagccg tgcggcacga tggggaagaa ttatcgggca
     1081 ggcgggcaac aatgaactga caaaagagga attgatccgg ctgcaacagg ctgttattga
     1141 aaatgcacgc tttgtcaagc ttggtttccg aaaacagggc ggctttgtgg gagaacatga
     1201 ccgtgtttcc ggagagcctg tcccggatca catttcagct aaatggcagg atgtggaaca
     1261 attaattgac gggctgatag cgtcaaatga cttgctgcta aagaacgaaa tggatgcagt
     1321 gctgactgcg gcggtaattg cattcggatt tgtattcata catccgtttg ttgatggaaa
     1381 tggacgtatc catcgttatc tgattcatca tgtattgatg aaaaaacaat tttccgcaca
     1441 ggggcttgta tttcctgtct cggcttccat tctgaatcac attgatgatt accgtaaagc
     1501 attggaaagc tattctttac ccttgctcga ttttattgaa tggaaagaaa ccagcgacca
     1561 caatgttgaa gttttgaatg atacaataga ttactacaga tattttgatg caacaaaact
     1621 tgcggaattt ctttatgatt gtgtgaacga cacaattcag aacatcattc ccgccgaact
     1681 gaaatatata cagcaatatg atgaatttaa aagatacatc gaaaatagat tcgaaatgcc
     1741 cgataaactc attgcaacac tggtccggtt tcttgaacag aacaaaggca aactatcaca
     1801 aagagcaagg aaaaaagaat ttgaagcttt gacggcaact gaaatcagag aaattgaaaa
     1861 atcatttgat gatatttttg gattgtaggg ttgattgaat gcgtgtctga atcaggcatt
     1921 caagactctg ttcggattta cggacttttg cttttcttaa aattctcagc ctgttcaaaa
     1981 aaccattttt cgtcctggca aagttgaatt atttcagaac caaatttcat ttcaacatta
     2041 cacgcatttg ctgaattcaa attacatttt caacgacttt ttaagaaact caagaaaacc
     2101 gtgtcccttt tgcgttaaga aatattgttg atttgggctg ctgggtttat tagggacagt
     2161 catctgtaaa aggcccattc ccaataaagg cgtgatgtat tttgccctga atttagttct
     2221 gcttttccag ttcagcagag tcatgaattc ctttacactt cttgatgttt gagaaaaaac
     2281 cagcaggttc tcaacctggt gccagcttag tgccagctta gtgccagctt ggtcccgacc
     2341 tggttccgac ctggtcccgg cctggtccca gcttagtccg aatatgacgg ctatttctct
     2401 tccaattgaa tcaaaaactg agttttgttg aatggctggc tggttgtctt ttttaataaa
     2461 ttgaacctga aattgcagcc cggtttcaaa ccatttaaat tcaatttccg gatacaattt
     2521 taattcctgc gagataatct tcaatccatt tccccattgg tcaataattc caagatgttt
     2581 aaaaacaggc gcaattactt tattccggat atcgctctga cgagcctcta aatcatcaaa
     2641 attgatggaa ggcagcaatg ttccgggact taaaatctca accatgtcgt cgtaaacagc
     2701 aactttaata tctttgcctg tcaggctgta atctctgtga gcaactgcat tacgaagtac
     2761 ttcgcggata gctttcattg gaaaagccca acggcttttt gtatatgcac catcaatgac
     2821 actttcttta tcgacatgcc gttcaacaaa tttcaatgct tcggccggtt gcagcccaat
     2881 ttgatcggtg atggttttat catccatttt agtttcggtg ctaacgcctt taaagcgcgc
     2941 acattccact tttgagtatg ggaagaggtc tttcctgctt tcataatcag cgaaaagggc
     3001 cagcgcattt gttggtaatt gtttcccccg gctttctttt atcagtaata gtttctttaa
     3061 agttgcctgc gtgaggtttt cgccggaaag ttccttaaat tgtttttcaa atgagcttaa
     3121 atcaaaatct gaaatggcat taccatgcag tggctcactg tcaaaagaaa tatttcgctt
     3181 acgtcgctgc aattcttcaa tgattgaatc atcggccagg cgattggttg aaccgatccg
     3241 gatatacgtt ccaccatgaa tgcctttaga tttcagatga tatgggaaat cgctgccacg
     3301 gtgtatttgc acacgaatta aataacaacc attttcaaca ggaaaagtga tatccggaac
     3361 tataacagga cagcagttgt catgaataat ggatgcaatt ttttcttcag ttttgagtag
     3421 ctcatcttca ggaatacctg tcaactttct gggattgttt tgaaccccga tatatacatc
     3481 acccccggca tcatttgcaa aagcaacaat ggtttttgtc agatcggcgc tggccggtag
     3541 tttctgctta aactccagcc gacggccctc gggtaatttt attatttcct gcaatttcat
     3601 taagatggag tcaagtataa taataattgc tgatttaatt taaattgcta atgtacaaat
     3661 tttaataacg gcatgcgatt gacgtgtgac tttccaactc acgggtgctt cgtctttaga
     3721 aaagcacatt tcattttcct cacaacatca atcccaaagt ggtcattttc gaccacttta
     3781 aaattcagat tttcaaaatt tcatccactt cccccatcac cctctccgtc tcattcagcg
     3841 ccacaataac acgctgatag tgcagaatat tatcgaaaga aagggtgcgg cctttgcggt
     3901 ctttgagcca tttctgggcg ggttggtagc cgccgatgta gaagttcca
//
LOCUS       DAOPKE010000163         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_27073_length_3757_cov_14.784711, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000163 DAOPKE010000000
VERSION     DAOPKE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27073_length_3757_cov_14.784711"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1300
                     /locus_tag="PKY63_12505"
     CDS             449..1300
                     /locus_tag="PKY63_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01480.1"
                     /translation="MIKILIALLFGLFLSNAVSAQYYMLGEDAANQRWYCIDTKHFHL
                     VFPESFLNRAQTLANQSEQYYRAITGTINHSNERIPVLIHTGSVVANAYSLWTPERTE
                     YFMTPSQNNYAHNWLLQLIIHEDRHMVQMDKLNQGLTKTFSYLIGDQAVAITLGLFMP
                     MWYMEGDAVTTETAFSESGRGRIPSFEMKMKAQLLTEGLYNFEKASLGSYKNYVPDQY
                     YFGYFFIAQSRKHYGPQLWDNAIRNSARNWWQISPLSHSFKQQTGYNKKQLYQLFFQD
                     LKDQWLL"
     gene            1331..3352
                     /locus_tag="PKY63_12510"
     CDS             1331..3352
                     /locus_tag="PKY63_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01481.1"
                     /translation="MVPLPESKVQTHYKFPEFINDSTIIAEKSGMGDYARFVTINIKT
                     GEEKTICKAGFYASEELPATEVIPVLSKNSPGAWTTDNLSLIQNTFVWAEKKWHPRWE
                     HVSYSVIMKHDLTTGKTKQLTHKSKYFSPALSANGKQITAVEFLPDGSCNIVILAAAN
                     GNLIDTYPLEPGAFAMNPEFSPDARQVVCITQKPDLKGIEILNLETKKWSTVLAPSAA
                     DISNPTWKNDGIYFNAPFSGTDNVYHLNQSTGAVSQVTFDTFGAYNISFSPSEERFIF
                     SSYQNKGFFLKTAQTNENNAQEMRLINKMILNPDSSYVLQEKAYMPDTLWKDSMYEIK
                     KYRRGFQLFDLHSWMPVSFNTDRYNLYPGITFSSQNLLSTSVLTGGFEMISDKYYART
                     FADWTYSGWFPQITVHASMGANEGIGNISEFEDHYIWHIKTAGLKISLPIKTTVRDFY
                     QKYRISAGLQTHELSPYNALVLPHSLPSGTTQSVDAELRISNRQKTVLRDLMPRWGQD
                     VSVHYQRSFGGDINLGRQWNADAKIFFPGLMKHHGFRIDAAYMEKTPGEYSFTDDFLN
                     PRGYRNIFSTHSQKIGLNYKFPLWYPDMNVGSLLYFKRLKLNLFYDYGKYKFQNSRHI
                     MNSADCEWTCDTHVFRFFIPLDIGFRYAHLPRTQENTFEFLLGINFSGF"
BASE COUNT         1126 a          857 c          767 g         1007 t
ORIGIN      
        1 cttaaatcag agcgagagcg aagatatctc tcttaatttc aaccagatta aagatttctt
       61 cagaaaaatt ctgtagcttt acgaagtgaa aacgatgcgg aaaatagaat gctttgggct
      121 gcgggctgag atgtccgctg tctccttcgg tcgggattgt cttgtgggac gccgggacct
      181 gtgggacagc agcggagctg ctgcattcgg catccggtct ggttttcggc ttgggagtga
      241 gccattttca cgctgcggac atgtggccat attcacatac atttatgtca aatcgcaccc
      301 ggcgatgtca gccccgcacg agctctctgc atcgcaactg cgaagcgctg cgatctgtcg
      361 cgctgaccgt cgtgagcgtc ggacttgaaa atgcaatagc tgtttcctat aaaaaacact
      421 aattttgtct atctttttga atccctgaat gataaaaata cttattgctt tactttttgg
      481 attatttctg tccaacgctg tcagtgccca gtattacatg cttggcgaag atgccgccaa
      541 tcaaagatgg tattgcatcg acacaaaaca tttccatctg gtttttcccg aatcatttct
      601 aaaccgcgca caaacgctgg caaatcaaag cgaacaatat tacagagcca tcaccggaac
      661 catcaatcac tccaatgaaa gaattcccgt tcttattcac acagggtctg tagttgccaa
      721 cgcgtacagt ttatggacac cggagcgcac cgaatatttc atgacaccat cgcaaaacaa
      781 ctacgcccac aactggctcc ttcaacttat catacacgaa gaccggcaca tggttcagat
      841 ggacaaactc aatcagggac tcacaaagac attcagttat ctgatcggcg accaggccgt
      901 agcaattacc ttgggtttat tcatgccaat gtggtacatg gaaggagatg ccgttactac
      961 cgaaacagct ttcagcgaga gcggacgcgg acgcattcct tcgtttgaga tgaaaatgaa
     1021 agcgcaactg ctgaccgaag gactttacaa ttttgaaaaa gcaagcttgg gttcatacaa
     1081 aaattatgtt ccggatcagt attatttcgg atactttttc attgctcaaa gccgcaaaca
     1141 ttatggaccg cagctctggg acaatgccat acgcaacagt gcacgcaact ggtggcagat
     1201 aagtccactt tcgcattcat tcaaacaaca gacaggatac aataaaaaac aattgtatca
     1261 gctgtttttt caggatctga aagatcaatg gctcttatag gcagcagcga atccgactgc
     1321 aaaatatgca atggttcccc tgcctgaaag caaggtacag acacattaca aatttcctga
     1381 attcatcaac gattcaacaa tcattgccga aaaaagcggt atgggtgatt atgcacgctt
     1441 tgtgacaatc aacatcaaaa ccggcgaaga aaagaccatt tgcaaagccg ggttttatgc
     1501 ttcagaagag ctgccggcaa ccgaagtcat tccggtactt tcgaaaaaca gtcccggtgc
     1561 atggaccacc gataacctga gtctgataca aaacactttt gtctgggccg aaaagaaatg
     1621 gcatccgcga tgggagcatg tgagctattc ggtgattatg aaacatgatc tcacaaccgg
     1681 taaaaccaag cagctcacac ataaatcaaa atatttcagc cctgctcttt cagccaacgg
     1741 gaaacagatt acagcagttg aatttctacc agatggatcg tgtaacattg tcattcttgc
     1801 tgctgcgaac ggaaatctga ttgacactta tccgcttgaa cccggtgcat tcgctatgaa
     1861 tccagagttt tcacctgacg ctagacaagt tgtttgcatc acacaaaaac ccgatctgaa
     1921 aggaattgaa attctcaatc tcgaaacaaa aaaatggagc actgttctgg ctccgtctgc
     1981 tgctgatatt tcaaatccaa cctggaaaaa cgatggcatt tatttcaatg cgcctttcag
     2041 tggaaccgac aacgtgtacc acctgaatca atctacagga gcggtttcac aagtcacttt
     2101 cgacaccttc ggggcatata atatttcatt cagtccttcc gaagaacgct ttattttttc
     2161 ttcttatcag aataaaggtt ttttcttgaa aacagctcaa acaaatgaaa ataatgctca
     2221 ggaaatgaga ctgatcaata aaatgatttt aaatccagac agttcatatg ttcttcagga
     2281 aaaagcatat atgcccgaca ctttgtggaa ggactcgatg tatgaaataa aaaaataccg
     2341 gcgcggattt caattattcg acctgcactc atggatgcca gtatcattca ataccgacag
     2401 atacaatctt taccccggaa ttactttttc gtcacaaaat cttttatcca cttcagtact
     2461 cacaggtggt tttgaaatga tcagtgacaa atactatgcg cgcacatttg ccgactggac
     2521 atactccgga tggtttccac agattactgt tcatgcatcg atgggagcca acgaaggcat
     2581 aggaaatata agtgaatttg aagatcatta tatctggcat atcaaaacag caggactgaa
     2641 aataagttta cccataaaaa caacagtacg cgacttttat cagaaataca gaatcagtgc
     2701 agggttacaa acacacgaat tgtctccgta taacgctctt gtgcttcccc actcacttcc
     2761 cagcggcaca acacaatcag tggatgcaga actaagaatt tcaaacagac aaaaaacagt
     2821 acttcgcgat ctgatgccac gatggggcca ggatgtttca gttcactatc agcgctcatt
     2881 tggcggtgat atcaatcttg ggcggcaatg gaatgctgat gcaaaaatat ttttcccagg
     2941 attgatgaaa caccatggat tcaggattga tgcggcttac atggaaaaaa ctcctggaga
     3001 atattcattc accgatgact ttttgaatcc gcgcggatac agaaatattt tttcaacaca
     3061 tagccaaaaa ataggactga attataaatt tccgttgtgg tatcccgata tgaatgttgg
     3121 gtcgttgctg tatttcaaac ggctcaaact gaatctgttt tatgattacg gtaagtataa
     3181 atttcaaaac tcaagacaca tcatgaactc tgcggattgt gagtggacct gcgatacgca
     3241 tgtgttccgt tttttcattc cgcttgacat cggatttcgc tatgcccatc tgcctcgcac
     3301 gcaggaaaac acctttgagt ttttgctggg aatcaatttt tcgggatttt gaattatgcc
     3361 actgagacac caagactcta tgatttacga ttcccgattg aagatttttg atttttgatc
     3421 tgaagtcaga acacagtgta gtgtctttct actttttgaa catgatacac ttttaactgc
     3481 aaagttcccg gtctcagatt gcgatgtagc ttctgctact gtaccgggat ctcctggaaa
     3541 accttcgagg ccctaacaca ctctcttccg cgaatgacgc tagtctccgc gaagaaactt
     3601 gtttccctca gcaggtctta tgaacgtaaa ttcgaatccg ccttcatctg cgatgaagac
     3661 tgattcaata tgtcgcgcag agagcgcagg gacgcagaga aattcgccac taagacacga
     3721 aagcacaaca tctttcttct gcgaatgaat ctaatta
//
LOCUS       DAOPKE010000164         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_28320_length_3637_cov_10.723897, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000164 DAOPKE010000000
VERSION     DAOPKE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28320_length_3637_cov_10.723897"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKY63_12515"
     CDS             complement(<1..529)
                     /locus_tag="PKY63_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01482.1"
                     /translation="MEVPHFREYIQGKFSKPLQSGCKYLFGFHVVYTESSIFASKLGI
                     GFTQDSVYYNTMDCLMSQGFYPDLETDVFITDTANWTKVEWIYTATGGERFFTIGNFW
                     CDTIESFQFTDSSGVLSNGLSGYFFDDFFMYELTDTMSIHNVYIPNVFSPDGNGQNDV
                     FRIRGAAISEIKTFLI"
     gene            complement(879..>3637)
                     /locus_tag="PKY63_12520"
     CDS             complement(879..>3637)
                     /locus_tag="PKY63_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPF01483.1"
                     /translation="LGSSVCFTELSNCEEYWHWNFGDGATSSQQNPCHDYAQAGNYLV
                     TLEVAPGIIVSHTITVYSPVQPIITGDESACESSSHYQVTPAGAFSQIGWAVNSPSGW
                     VSTVPPDEYNHSWTNGTGGVVYVETTDNNGCVAHGEMMVFDCCANPETTISFFNETLT
                     SPQTYWHEVISVNGTLTIDANGTTSFYDCHFYMGVNAKIVITSNDIVNIGYHTKNAPQ
                     TIIEACDSLMWDGIYINDNTAKLYVYYSKIYDAKNAVVSLHGGVFEIKTSLFDKNYKH
                     LDIRTYNGTHQGSITESVLTCSGTLLPQYPFIQGATKTRYAVSISRASNVTIGDPQSI
                     SLQNTIEKCDVGIYCLNSGVKVYNNKFQNISVPVNLPISVKKGFGIFAEKAVNYISPV
                     SSLTVGGSGNLPGVGNFRRNVFIDVTNGVYTYQYSNIAINNNTFEIQNPTTAESKALM
                     IYDYAHFAGTKSITNNTISNYRTGVFLNDVESMSVRDNDISDFQISQTGGYSYGIRML
                     GGNDVLIYGNTIENSSIYANTEGIRVSTSPLSTVQCNTIKNMGKSIHLQGTMMPFTLR
                     QNRMENGIDALVFTNATIGTQGTSNNPQGNTWYGSFSNSQTLMNQSYGSNSQFYIKNG
                     NHTVPAINLIVYPGDVFSPIFTQSNTWPGCVQVIPAAANPSSLYSIANNQIGYAQFEQ
                     ENKWMARKQVLLAMRKDSISGDPFLSQFSLSQQNTGTAISLLVEDTLKVDPATAEILN
                     SLMPETNFNETATKLVNNVFFEKEADTTMLPADLNISSLSADSLRWLAQQCPFEFGKA
                     VYQARMLLSLIDTTEYMNLCEAGSAGFSDKSVQSFSTDSFNISVSPNPFANELKIEFE
                     NPEECTGFVCLMDLTGKVIATYELPEKASQFVIQTDEIAAGIYYLKVWNDNNLLKYSK
                     IVKY"
BASE COUNT         1085 a          757 c          715 g         1080 t
ORIGIN      
        1 aaatgagaaa tgtttttatt tctgatatag cggcacccct gatgcggaac acatcgtttt
       61 gcccattgcc atccggcgaa aaaacattgg gaatatacac attatgtatg ctcatggtat
      121 ctgtaagttc gtacatgaaa aaatcatcaa aaaaataacc tgacagacca ttgctaagaa
      181 cacctgaaga atctgtaaac tgaaaacttt ctattgtatc gcaccagaaa tttccgatgg
      241 taaaaaaccg ttcacctccg gttgccgtgt atatccattc cactttggtc cagtttgccg
      301 tatctgtaat aaatacgtcc gtttccaaat cgggataaaa cccttgcgac atcaaacaat
      361 ccatggtgtt gtaatatact gaatcttgtg tgaaacctat gcctaatttt gacgcaaata
      421 tacttgactc cgtatataca acatgaaatc cgaataagta tttacaacca gattgtaatg
      481 gtttcgagaa ttttccctga atatattctc tgaaatgcgg aacttccata aactcatatt
      541 tacaacaggc tatatggcaa taggcttctc ctgtaagagg atattgataa ccaattccgg
      601 atgtcggaga agtgataaca agaggaggat ctaattcgct gtgtagataa tccggagatc
      661 cattcgtcgg atttgaccaa tcagtcaccc tgcatccata aattacagaa ggcaattcat
      721 cattataaaa gtagattact ccacaaagtg aatctctaca ataaccctcg aaagatgtat
      781 ttaacaccag gttctgagaa gaaatcccac tcggttggaa aagcataaat ataaacatca
      841 ggagtgcaaa aatccgcacc cctgatgtaa tatcacaatc agtatttgac aatttttgag
      901 tatttaagga ggttgttgtc attccacact ttcagataat agattcctgc ggcaatctca
      961 tcggtctgaa ttacaaactg tgatgctttt tcagggagtt cataagttgc aattactttt
     1021 cccgtcaaat ccatcagaca tacaaaaccg gtacactctt ccggattttc gaattcaatt
     1081 ttcagctcat ttgcaaaagg atttggcgaa acgctgatat tgaaagaatc agttgaaaat
     1141 gactgcacag acttgtcgga aaatccggcc gaacctgctt cgcataaatt catgtattca
     1201 gttgtatcta tcaacgagag aagcatgcgg gcctgataaa cggctttgcc gaattcaaag
     1261 gggcattgct gggcgagcca ccgcagactg tcagccgaca aggacgaaat attcagatcg
     1321 gcggggagca tggttgtgtc agcttctttt tcgaaaaaga cattatttac aagttttgta
     1381 gcggtctcgt tgaaattggt ttcgggcatc agcgaattca atatttcggc agttgcagga
     1441 tccactttca gcgtatcttc aaccaacaac gaaatggcgg tgcctgtgtt ttgctgactc
     1501 agagagaact ggctgagaaa aggatctccg gaaatcgaat ccttcctcat ggccagcaac
     1561 acctgcttgc gagccatcca tttgttttcc tgttcgaatt gagcatatcc gatctgattg
     1621 ttggcaatac tatacaacga tgaggggttt gctgctgcag ggataacctg tacacaacct
     1681 ggccaggtgt ttgactgagt aaaaattggt gaaaacacat caccaggata tacaattaaa
     1741 ttaatagcag gaacagtgtg gtttccattt ttaatataga attgcgaatt actcccataa
     1801 ctctgattca ttaatgtttg agagtttgag aaactcccat accatgtatt tccctgcggg
     1861 ttattggatg ttccctgggt tcctatggtt gcattggtaa acacaagagc atcaataccg
     1921 ttttccatac ggttttgcct caaagtgaac ggcatcatcg tgccttgcag gtgaatggat
     1981 tttcccatat tctttatcgt gttgcattgc actgtggaaa gcggagaagt gcttacacga
     2041 atgccttcgg tgtttgcata gatggaggaa ttttcaatag tgtttccata aatgagcaca
     2101 tcgtttccgc ccagcatccg aattccgtat gagtatccgc cggtctgtga tatctgaaaa
     2161 tcgctgatat cattgtcgcg aacactcata ctttcaacat cgttcagaaa aacaccggtc
     2221 cggtagttgg aaatagtatt gtttgtgatg ctttttgttc cggcaaaatg agcataatca
     2281 tatatcatca aagctttgct ttcggccgtt gtcgggtttt gaatttcgaa cgtattattg
     2341 ttgatagcaa tatttgaata ctgatatgtg tacacgccat ttgttacatc aataaacaca
     2401 ttcctcctga agttacctac tcccggcagg ttgccactac ctccaacagt aagggaagaa
     2461 acaggcgaaa tataattcac agctttttcc gcaaagatac caaacccttt tttaaccgag
     2521 attggtaagt ttacaggcac tgaaatattc tgaaatttat tgttgtaaac ttttactcca
     2581 ctattcaggc aatatatgcc gacatcgcat ttctcaatag tgttttgcaa tgaaatgctt
     2641 tgcggatctc cgatcgtcac attcgaagca cgggaaattg aaactgcata tctggttttg
     2701 gttgccccct gaataaatgg atactgtggc aataatgtac ctgaacaagt caaaacgctt
     2761 tctgtaattg aaccctgatg tgtgccgtta tacgttctga tgtcaagatg cttgtaattc
     2821 ttgtcaaaaa gagatgtttt aatttcaaat actcccccat gcaatgaaac aacagcattt
     2881 ttcgcgtcgt aaattttact gtaatataca tacagcttgg ctgtattatc attaatataa
     2941 attccatccc acattaatga atcacaggct tcgataatag tttgcggagc atttttcgtg
     3001 tgatatccaa tgttaacaat gtcgtttgaa gtaattacaa tttttgcatt tacacccata
     3061 tagaaatgac aatcgtaaaa ggaagtagtc ccattagcat caatggttaa agtgccgttt
     3121 actgaaatca cttcgtgcca atatgtttgc ggtgaagtga gggtctcgtt gaaaaacgaa
     3181 atagttgttt cgggatttgc acaacaatca aacaccatca tctcaccatg tgcaacacaa
     3241 ccattattat ccgttgtttc aacatacaca acaccacctg ttccatttgt ccatgaatga
     3301 ttgtattcgt caggcggaac cgttgatacc cagccggaag gtgaattcac cgcccagccg
     3361 atttgcgaaa atgcaccggc cggagtaacc tggtaatgac tgcttgattc acatgctgat
     3421 tcatcacctg taattattgg ctgaacaggt gaataaacag ttattgtatg agagactata
     3481 atccctggtg caacttcgag cgttaccaga taatttcctg cctgagcata atcgtgacag
     3541 gggttttgtt gtgaactggt ggcgccatca ccaaaattcc aatgccagta ttcttcacaa
     3601 tttgacaatt ctgtaaagca aactgaactg ccgagac
//
LOCUS       DAOPKE010000165         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_28753_length_3600_cov_10.486037, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000165 DAOPKE010000000
VERSION     DAOPKE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28753_length_3600_cov_10.486037"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKY63_12525"
     CDS             <1..332
                     /locus_tag="PKY63_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF01484.1"
                     /translation="LTPQTKELVNKDTLALMKPTAVLINTARGGVVNEQDLADALNDG
                     KIAAAGLDVLTVEPMKADNPLLKAKNCVITPHTAWASKEARQRLIGMVAGNIKAFMDE
                     KPINVVS"
     gene            590..1078
                     /locus_tag="PKY63_12530"
     CDS             590..1078
                     /locus_tag="PKY63_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01485.1"
                     /translation="MAKGPLILKISGIVMFIEALIGSSIALFSGLSLISMLNKRSEMR
                     EQLLSPLGYLSFAIFGLVLLTSIALFVCAMKSFHTYNHYLVPGGNDNYLVPSRYVLIL
                     GALTFLSGFAVTYTPNGNRSYAVLIMIASTGWVFALLNLILVKKDREERAKEEAQPVR
                     RF"
     gene            1263..2507
                     /locus_tag="PKY63_12535"
     CDS             1263..2507
                     /locus_tag="PKY63_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01486.1"
                     /translation="MKKKNVLVLSVLFVFASSFLMSCGNKVNVVKDIPSGFVKLNIAE
                     DNLDVGGLHYTIGESQISFLDADEICTYQTELKDTQFIVKHENEYYVNEKKYSELVAM
                     AKGVTVQRNTQYSNGDTIELRGIGLIPYTVEFFDVLSYSDGKIKLCRIRFIMGPNIPE
                     NKQSQIFSYIETDKGTIIKDYEYTAGIFEVLIPVEEKIKIIVIKSPDYPGCIRKIING
                     SNIKVVQTKPEGYIQLDKATDNHGNAIDYMVFENGITFFDADRIGIYGAVIYDQSFLV
                     IYEDEWYVNEKKYLEIVKISDAVSEKRNKIYSIGDDVELRGCDVNYKDGYSIKINEVK
                     ADTDGSIYTIQFSISPYVTEYKRKEIFDHVETDRGTIINEFTFIGDAEVQVTVPVGEK
                     INMIVVKSPDYDHAIRKINVSY"
     gene            2538..3479
                     /locus_tag="PKY63_12540"
     CDS             2538..3479
                     /locus_tag="PKY63_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01487.1"
                     /translation="MKPIKVFLRIILVVLAFFLLSCERSVAEVVERIPPGFVKLSTAG
                     NNLYNGGLHYTIDESQISFFDADEICIYQSELTNDDFIVVYENEYYIYQGKYLELIDK
                     STDAKVVETIPDGFIQLNVGLRSLKNKLHYVLGETEILFLSADEICIFQTRLNNNKYI
                     VLYENEFYFNEQKLAELINQATLIDQQREMIYSGSVNLPARGTPYTIMLFDVIESIGT
                     EGNTLCNLEFASSVSGDCVEGYGTDIMKQIFDHVETNKGTIIDEFTIDNIRGDIQVEI
                     PAGEELSIIAVRSPDYPGFVRKISSSSRGKSLLTWPN"
BASE COUNT         1147 a          599 c          842 g         1012 t
ORIGIN      
        1 cgttgactcc gcagaccaaa gagctggtca acaaggatac gctcgcgttg atgaagccga
       61 ccgccgtgct tatcaacact gcacgcggcg gcgttgtgaa cgagcaggat ctggctgatg
      121 cgctcaatga cggaaaaatc gcggcggcgg gtcttgacgt gctgaccgtt gagccgatga
      181 aagccgacaa tccgctgctc aaagctaaaa actgtgtgat cacgccgcac accgcatggg
      241 cttccaagga ggcgcgccag cggttaatcg gcatggtcgc gggcaatatc aaggcgttta
      301 tggacgagaa acccatcaat gtcgtcagtt gaaagtgtaa aatgtaagtg gtaaagtgag
      361 cagttggtag tgaaaagtgg acagcgacat atccggagtc cgttgtactg agttcaataa
      421 atgaattatg agacagcaaa agaagcgtcg ctttgatagc ggcgcttttt atttctgcaa
      481 cagcatattg acgcgtgaaa aaatcgggta tatgatataa atagaaagat attgagtgaa
      541 ttcaaacagc ggtgttttca taagatgcag tgcggtgggg gtgaagatca tggcaaaagg
      601 gccgttgatt ttaaagattt cggggattgt catgtttatc gaagcgctca ttggatcatc
      661 cattgcgctg ttctcgggac tgtcgctgat ttctatgctg aacaagcgta gcgagatgag
      721 agagcagctg ctgagccctt tgggctacct gagttttgcc atattcgggt tggtgctgct
      781 cacttcaatc gcgttattcg tttgtgcgat gaaaagcttt catacatata atcattatct
      841 ggttcccggc ggcaacgata actatttggt acccagccgg tatgtgctga tcttgggtgc
      901 gctcacgttt ctgtcgggtt ttgccgtgac atatacgccc aacggcaata ggagttatgc
      961 cgttttgata atgattgcct ctaccgggtg ggtgtttgcg ctgctcaatt tgatccttgt
     1021 taaaaaggat cgggaagagc gcgcgaagga agaagcacag ccggtaagga ggttttagag
     1081 taagtgaaca gttggcagtt taaagtgaaa agtgaatttt cgtaaatgca aagcgtcgta
     1141 gtaacggcgc tttactttta tccgaggaat atattgacat ttcagcgata aagggttatg
     1201 ataaaaaagc gggtatagaa atgcaaaatg tattggatta tatataattt atggagaaca
     1261 acgtgaaaaa gaaaaacgtg cttgtgttga gtgtcctttt cgtatttgct tcttctttcc
     1321 tgatgtcttg cggaaataaa gtaaatgttg tgaaagacat accgtccgga tttgtaaaat
     1381 taaatatcgc agaagacaat cttgatgtcg gcggtctgca ttacacaatc ggcgaatcac
     1441 agatttcgtt tttggatgcc gacgagattt gcacctatca aacggaattg aaagacactc
     1501 aattcattgt aaaacacgaa aatgaatatt atgtcaatga aaaaaagtat tcggaattag
     1561 ttgctatggc aaaaggggtc actgtgcaac gaaacacgca atacagcaat ggagatacca
     1621 tagagcttcg cgggattgga cttattccat atacggtgga atttttcgat gtgctgtcat
     1681 acagtgatgg taaaataaaa ttatgccgta tccgattcat aatgggccca aacatccctg
     1741 aaaataaaca gtcgcaaatt ttcagctaca ttgaaactga caaaggaaca attattaagg
     1801 attatgaata tactgcggga atatttgaag tgttgattcc tgtagaagaa aaaataaaaa
     1861 tcattgttat aaagagccct gattatccgg ggtgtatccg aaaaataatt aatggcagta
     1921 atattaaagt ggttcaaaca aaaccggaag ggtatattca attagacaaa gcgacggata
     1981 atcatggaaa tgccatagat tatatggtct ttgaaaatgg gattaccttt ttcgatgctg
     2041 atagaatagg tatatatgga gcggttattt atgatcaaag ttttcttgtg atatacgagg
     2101 atgaatggta tgtaaatgaa aaaaaatatt tagaaattgt aaaaatatca gatgctgtct
     2161 cggaaaagcg aaacaaaatt tactccatag gagatgatgt tgaattacga ggatgtgatg
     2221 tcaactataa agatggatat agtattaaga ttaacgaagt aaaagctgac actgatggat
     2281 ctatatacac aattcaattt tcgatcagcc cctatgtaac cgaatacaaa cgaaaggaaa
     2341 tctttgatca tgttgaaact gatcggggaa caattatcaa tgagtttaca tttatcggcg
     2401 atgcagaagt tcaggtaacc gttccggtcg gtgaaaaaat taatatgatc gttgtaaaga
     2461 gtccggatta tgatcatgcc attcgaaaaa taaacgtctc atattaaaat cagcataaaa
     2521 agcatcagga gggcacaatg aaacccataa aagttttttt acggatcatc cttgtcgtgc
     2581 tcgctttttt cttgttatcg tgtgagagaa gtgttgcaga ggtggttgaa agaataccgc
     2641 cgggatttgt gaagttgagc actgcgggaa acaatcttta taacggcggt ctgcattata
     2701 caatcgatga atcacagatc tcattttttg atgccgacga gatttgtatt tatcaatcag
     2761 agttgaccaa tgatgatttt attgttgtgt atgaaaatga atactatatt tatcaaggca
     2821 aatatttaga attgattgat aagtcgaccg atgccaaagt ggttgaaaca ataccggatg
     2881 gatttattca attaaacgta ggattacgca gccttaaaaa caagctgcat tatgtgctgg
     2941 gggaaacgga gattttattt ctatctgccg atgagatttg catcttccaa acaaggttaa
     3001 ataataataa atatatcgtc ttatacgaaa atgaatttta tttcaatgag caaaaacttg
     3061 cagagttgat caatcaagcg acattgatcg atcaacaacg cgaaatgata tacagcggat
     3121 cggtgaatct gcccgcaagg ggaactcctt atacaataat gctatttgat gtaattgaaa
     3181 gcatcggtac agaaggcaat accttatgta atcttgagtt tgcaagcagt gtttcgggag
     3241 attgtgttga aggctacgga acggatatta tgaaacagat atttgaccat gttgaaacga
     3301 ataaagggac aatcattgat gaatttacga ttgataatat cagaggagat attcaggtgg
     3361 aaattccggc aggtgaggaa ctcagcatca ttgcagtaag aagccccgat tatcccggat
     3421 ttgtccggaa aataagcagc tcatcaaggg gtaaatcttt attaacatgg ccaaattaac
     3481 attttgatcg gatgcgcttt ctagtactat atagattctc ctataaaaat aaagtgacat
     3541 tccgacgcga tgattcttcg ggaatgtcac tgttcactgt aaactgttaa cttttcacta
//
LOCUS       DAOPKE010000166         3437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_30728_length_3437_cov_8.988468, whole genome shotgun sequence.
ACCESSION   DAOPKE010000166 DAOPKE010000000
VERSION     DAOPKE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30728_length_3437_cov_8.988468"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKY63_12545"
     CDS             complement(<1..192)
                     /locus_tag="PKY63_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01488.1"
                     /translation="MKIDNCHIPNFVARGLCVHQKDNVSEMNFSSGCCKVSAETAFNG
                     IKNMGTALQFDCVEVRFKNN"
     gene            complement(1080..2228)
                     /locus_tag="PKY63_12550"
     CDS             complement(1080..2228)
                     /locus_tag="PKY63_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HPF01489.1"
                     /translation="MLFRDIPGNTKVINKLITSAEEQRIAHAQMFIGPPGSGKLMMAL
                     AYAQFLNCTDRKYYNDADGLKADSCGECSSCRRFASMEHPDLHFLFPVVKADKRELSR
                     DYMADWRTFVKNKAGIVNLNSWYKHIQVENKQGLISVDDINDIIGRLQYKNHEAKVKV
                     IIVWMAEKIQYNAAPKILKTLEEPSDNTLFILIAENHDLILPTILSRVQTTRIEAPTS
                     QQSYSFLREHYSDRPENDIFDAIYNSEGNLEKAYDYLQDSDEMKDVSDFFRKWMRTCF
                     ALNGKKIFTMSGEFAEMGREKQKHILAESARKLTNTFQLGADLKKIKFYDEEDEKFFS
                     KFATYVHKDNLDFFCNSIDEAIYHVERNGNPKLIFTDLSFDIGRMFRL"
     gene            2540..>3437
                     /locus_tag="PKY63_12555"
     CDS             2540..>3437
                     /locus_tag="PKY63_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPF01490.1"
                     /translation="MRKLFLLSWLFIFVFATTTIAQDEGYKFTDTKTVKATSVKDQYR
                     SGTCWSFSALGFYEAELLRLGKGEFDLSEAFVIRHAYIDKAERYLRFHGALNFGGGGA
                     FHDVTNVMKTYGMVPESAYPGLNYGTEKFDHSELDAVLKGYMDAIVKNEDAVPTTAWK
                     NGFIAILDAYLGPEPTEFEYNGKKYTPKSFQDMLGLNFDDYVEVTSFSHHPFYQEFIL
                     EVPDNWSHDMSWNIAVNELVEIIEYSINNGYTVAWASDVSEKGFSWTNGVAVVPDAKR
                     DDLSGTEREKWEKLTAKEKAAQL"
BASE COUNT          992 a          711 c          778 g          956 t
ORIGIN      
        1 attattttta aatctgacct caacacaatc aaactgcaag gcagttccca tgtttttaat
       61 gccgttgaaa gccgtttcag cagacacttt gcagcaaccc gatgaaaaat tcatctcgct
      121 tacattgtcc ttctgatgaa cgcaaagccc acgggcaacg aaattcggaa tatgacagtt
      181 atctatcttc ataatataag ggtgcaaatg tacaatataa tgtggaaata cgaaatacga
      241 gacacgaaat cagaaatacg aaatgatgtg gaaatgtggg acttcacgtg aaacgtgaca
      301 cggtcgtcca tgtgtcgctc acgtgaaacg tgacaagttc gcgaatgtgg gaatattaga
      361 tgatcacttt ttctttatac ttttatcttt atacttttat cttttgtctt caggggatgt
      421 gggaatgtgg gaatacgaga tacgaaatac gaaatacaag atacgaaatc agaaatacga
      481 gatacaaaat acgaatgaat gtgaagatgt gtcgcttcac gtaaaacgtg acacagtcgc
      541 tcatgtgaca ccttcacgtg aaatcacgta aaacgtgaca agctgtgata aatcggtgta
      601 tgtgtaaata tgagattctg gaaaaaatgt gggaatttgg gaatatagat ggtcactttt
      661 gtctttttcc acttcgacaa gctcagtgca gcgcttttat cttttgtctt gaacggatgt
      721 ggaaatgtgg gaatacgtaa taagagatac gaaatcagaa atacgagata tgaaatactg
      781 cagcgatgat gcgatgatgc gataatgcga tgatgtggaa atgtgcgcct ccggcgatgt
      841 gcaaatgtgg aaatacgaaa tacgagagag gcgtaagtca taaggagtaa ggtgtaaggc
      901 gataggcatt gagctttgcg ttgtgcgtgg agtatatcac gccgaaggcg ggacgttgtg
      961 ccacgaaccg catgccgcct gccacaagcc aaatacagag cgagaatcag agcaccgacg
     1021 cttcggtcgg cacatattgc tgatcgctga tgactgacgg ctgaaagctg tgaagtaaat
     1081 caaagccgga acatgcggcc gatatcaaag ctcaaatcgg taaaaattaa tttcggattt
     1141 ccgttgcgtt caacatgata aatggcttcg tcaatgctat tgcagaagaa atcaagattg
     1201 tccttgtgaa catacgtcgc aaatttgctg aaaaactttt catcctcctc gtcgtagaac
     1261 ttgatttttt tcaggtcggc accgagttga aaagtatttg tcagtttgcg ggcgctttca
     1321 gcaagaatat gcttttgttt ttcgcggccc atttcggcaa attcgcccga catagtaaaa
     1381 attttcttac cattcaatgc aaagcatgtc ctcatccatt tgcggaaaaa atcagaaaca
     1441 tccttcattt catcgctgtc ctgcaggtaa tcatatgctt tttcaaggtt cccctcactg
     1501 ttgtaaatgg catcgaaaat gtcattctca gggcgatcgg agtaatgttc acgcaaaaaa
     1561 ctataactct gctgtgaggt cggggcttcg attctggttg tctgtacccg tgaaagaatg
     1621 gtcggcagaa tcaggtcgtg attttctgca atcagaataa acaaagtgtt gtctgatggc
     1681 tcctccagtg tcttcagaat tttcggggcg gcattgtact gaattttttc agccatccag
     1741 acaatgatca ctttcacctt tgcctcgtga tttttgtact gcagccggcc gatgatatca
     1801 tttatgtcgt caaccgaaat taggccctgt ttgttttcaa cctgaatatg cttgtaccat
     1861 gaattcagat tgacaatgcc cgccttgttt ttgacgaaag tgcgccaatc ggccatgtag
     1921 tcgcggctca gctcacgttt atctgccttc accaccggaa acaggaaatg cagatcgggg
     1981 tgctccatcg aagcgaagcg ccggcacgac gaacattcac cgcagctgtc ggctttgagg
     2041 ccatcggcat cgttgtaata ctttcggtcg gtgcaattca aaaactgggc ataagccaga
     2101 gccatcatca gcttgccact ccctggcggg ccaatgaaca tctgcgcatg ggctatacgc
     2161 tgttcttcag ctgatgtaat cagcttgttg atgacttttg tgttgcctgg aatatctctg
     2221 aaaagcatgc gaaaattttc aacgaaaata cggcaaaaaa aattattgtc agtaaaaaat
     2281 gcaatccata aaatatttga cacaatactg ccgttataat ttgcagagtg agttccaaaa
     2341 actgttcttt tcaaaaacct tttgtttgga tagctctcat ggtgggtaat gtgatgcccg
     2401 gggccaatac tccgggcatt tttcatttcc ggtttggtcg ggataatcgg gcgataagta
     2461 tatagttaaa gctgatatgc ggttttaaca ttggtcaccc gataaaaaaa actatttttg
     2521 tatcaataaa tgatatgtca tgagaaaact atttctattg agctggttgt tcatcttcgt
     2581 ttttgcaaca accaccattg cccaggatga agggtataaa ttcaccgaca ccaaaaccgt
     2641 gaaagcaaca tccgtcaagg accagtaccg ctcgggcacc tgctggagtt tttcagcact
     2701 gggtttttac gaagcagaac tgctgcggct tggcaaagga gagtttgatc tttccgaagc
     2761 ttttgtaatc cgtcatgcct atattgacaa agccgagcgc tatcttcgtt ttcatggcgc
     2821 gctgaatttc ggcggtggtg gcgcttttca cgatgtcacc aatgttatga aaacctatgg
     2881 aatggttccc gaatcagcat acccgggact gaattacgga accgagaaat tcgatcacag
     2941 cgaactggat gccgtactca aaggttatat ggatgccatc gtcaaaaacg aagatgctgt
     3001 accaaccact gcatggaaaa acggcttcat cgctattctt gatgcatacc tcggaccaga
     3061 gccaaccgaa tttgaataca atgggaaaaa atatacaccg aaatcatttc aggatatgct
     3121 tggactgaat ttcgatgact atgttgaagt tacatcattc tctcatcatc ctttctatca
     3181 ggaattcatt ctcgaagttc ccgataactg gagccatgat atgtcatgga atattgccgt
     3241 caatgagctt gttgaaatca tcgagtattc gatcaacaat ggttacactg ttgcctgggc
     3301 cagcgatgtt tcggaaaaag gtttcagctg gacaaatggt gtggctgttg ttcccgatgc
     3361 aaaacgtgat gacctctccg gtactgaacg tgaaaaatgg gaaaaactca ctgccaaaga
     3421 aaaagctgct cagttgt
//
LOCUS       DAOPKE010000167         3435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_30760_length_3435_cov_13.118639, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000167 DAOPKE010000000
VERSION     DAOPKE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30760_length_3435_cov_13.118639"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKY63_12560"
     CDS             complement(<1..979)
                     /locus_tag="PKY63_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01491.1"
                     /translation="MKKITLLCIAVLMTVFTAIGQTIVLNEDFESAPYELSSSGTVSW
                     GINSRLHASGSFSDSCTVGLSSTSYLTTNSFSTVGNTSVLLEFKHICKIESGDAGQVE
                     YSIDGGTTWTLITNAYYLGTGSFTTNKFNEGSYVADWQFGVGTAVPQNSWWKTEQFDL
                     GTLIGNQANVMIRFKLNDANNNGANQRTGWYLDDVKVTVAPSELVPPTITLVPVIYQD
                     TVYTTGPFEIKAAISDASGIDTATLTYSINGGSNIILPMTVLLADTFHATIPAQAYNT
                     RIDYSISATDASLAANTGTTTGNWFFIKKPAPVAIIGTGTSTTSYLPAYG"
     gene            1101..3341
                     /locus_tag="PKY63_12565"
     CDS             1101..3341
                     /locus_tag="PKY63_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPF01492.1"
                     /translation="MEFGLTEIEKKELLKRYRALLKVCKPITDSKDKRLIRKAFNLAA
                     EAHKGMRRRSGEPYIFHPMEVAVISAGEIGLGTTSIICALLHDVVEDTDYTLEDISGL
                     FGPKVASIIDGLTKISGLVDNSDFGPSIQAENFRKILLTMAEDVRVILIKLADRLHNM
                     RTLEHMPPEKRLKIASETAFLFAPLAHRLGLYTIKSEMEDLVLKYTEPDVYESISQKL
                     IETEEERRKFIHQFIKPVEALLKKEDLNFNIIGRTKSINSIWKKMLKKNIPFEEVYDI
                     FAIRIIIDSDDVHKEKIDCWKAYSLVTQVYTPNPERLRDWISVPKANGYEALHTTVMS
                     DAGKWVEIQIRSFRMNEIAEKGYAAHWKYKENKDGMENSLDDWLKKIRELLQRPEANA
                     LDFLDDFKLTLFSDEIYVFTRTGELRNLPKNSTILDFAYSIHTDLGNRCIGAKVNHKL
                     VPLSHVLNSGDQVEVLTSDKQSPKEEWLQSAVTAKAKSQIKMAVKEERRKAIDRGRDI
                     VLRMFGQVKLVSEEPVLLEFAKYTGVQSVSELFFEASHGKIDIKMVKAFATVHDRSGW
                     LQYLRMPFVRGKSGIKPGTERSRFIRKSKGVELNVDSKISYTIADCCNPIPGDEVVGF
                     VVSENTLEIHRANCANAVRISARYGDRIKKVQWNDSKLITFKTAIQVSGIDKIGILRD
                     ISRILSEENNLNISSIFLESKGETFEGRIILFVNDTEHLKNLIAQLKKVDGVQRVFRL
                     RLSENQ"
BASE COUNT          989 a          696 c          810 g          940 t
ORIGIN      
        1 aaccgtaggc cggcaggtat gaggttgttg aagtcccggt accgataatg gctacgggtg
       61 ctggtttctt gataaaaaac caattgcctg tggttgttcc tgtgtttgca gccagtgagg
      121 catcggtggc cgaaatggaa tagtcaatgc gggtattgta tgcctgagcc ggaatggtgg
      181 catggaacgt atcggccagt aaaacggtca tcgggagaat tatattggag ccgccgttga
      241 ttgaataagt aagggttgcg gtatcaattc ccgaagcatc gctaatggca gctttgattt
      301 caaacgggcc agttgtgtat accgtatcct gataaatgac cgggaccagg gtgatggtcg
      361 gcggaaccag ttcggacggt gcaacagtaa ctttcacatc atccagatac cagccggttc
      421 gctggttggc gccgttgtta ttggcatcgt tgagtttgaa gcggatcata acattggcct
      481 gattgcctat caatgtcccc agatcaaact gttcggtttt ccaccaggag ttttgtggaa
      541 cggctgtacc tacaccaaac tgccagtcgg caacatagga tccttcgttg aatttgttgg
      601 tggtaaatga accagtacct agataatagg cattggttat cagggtccaa gtagttccac
      661 cgtcgataga atattctacc tgacctgcat ctccgctttc aatcttgcag atatgcttga
      721 attcaagtaa aacgctggtg ttaccaacag tcgaaaacga gttggtcgtc aggtaactgg
      781 tgctagataa gccgacagtg caggaatcac tgaatgagcc cgacgcgtgc agccgtgaat
      841 tgattcccca ggaaacagtc cctgatgaag agagttcata cggtgcagac tcaaagtctt
      901 cattgagaac aatggtctgg ccaatggcag taaatactgt catcagcact gcaatacaga
      961 gcaatgtaat ttttttcata attgggttgt ttacagatag cgttgcaaat ataatcaatc
     1021 tttataaaaa taataatgat ttatcaattt ttcagattat ttgcactctt tcttttaatt
     1081 cttatttttg caataaaaaa atggagtttg gccttacgga gatagaaaag aaagagttgc
     1141 tgaagcgtta ccgtgcattg ctgaaggtgt gcaaacccat tacagactca aaagacaaac
     1201 gattaatacg caaagcgttc aatctggctg ccgaagctca caaaggcatg cgacggcgta
     1261 gtggagaacc ctatattttt catccgatgg aagtggccgt tatttctgcc ggtgaaattg
     1321 gtctgggtac aacttctatc atttgcgccc tgcttcacga cgttgtggag gatacagatt
     1381 acacactcga agatatcagc gggttgtttg gaccaaaagt agcttcgatt atagatggac
     1441 tgacaaaaat ttccggattg gttgataatt ccgattttgg cccctctatt caggccgaaa
     1501 attttcggaa aattttgctc accatggccg aagatgttcg tgtcatttta atcaaactgg
     1561 ccgatcgttt acataatatg cggacattgg agcatatgcc tcctgaaaaa agactcaaga
     1621 ttgcttctga aacagctttt ctttttgccc cgctggcaca tcgtctgggt ctttatacca
     1681 tcaaaagcga gatggaagat ctcgtcctga aatatacgga gcccgatgtg tatgaatcca
     1741 tttctcaaaa actcattgaa acagaggaag aacgaagaaa gtttatccat cagtttatta
     1801 aaccggtcga agcgctgctg aaaaaagaag accttaattt taatatcatt gggcgtacaa
     1861 aaagcatcaa ttccatctgg aagaaaatgc ttaaaaagaa tattccgttc gaggaggttt
     1921 atgatatttt tgctatccga attattattg attcggacga tgtacataag gaaaaaattg
     1981 attgctggaa agcttattca cttgtaactc aggtttatac acctaatccg gagcgcctgc
     2041 gagattggat ttcggtgccc aaagccaatg gctacgaggc cttgcataca acagttatga
     2101 gcgatgccgg caagtgggtc gaaattcaaa tccgttcttt ccggatgaat gaaattgctg
     2161 aaaagggcta tgccgcccat tggaaataca aggaaaacaa agatggcatg gagaattcgc
     2221 tcgacgattg gttgaagaag attcgcgaat tgctccagcg tccagaggcc aatgcgcttg
     2281 attttcttga tgatttcaaa ctgactcttt tctccgatga aatatatgta ttcacacgaa
     2341 ccggtgaact gcgcaatttg ccgaagaact caacgattct tgattttgct tattctattc
     2401 atactgatct cggaaaccgg tgtattggcg caaaagtcaa ccacaagctt gttccattga
     2461 gtcatgttct caacagtggc gatcaggttg aagtgcttac cagcgacaaa cagtcgccga
     2521 aagaagaatg gctgcaaagt gccgttacgg ccaaagccaa atcgcaaata aaaatggctg
     2581 taaaggaaga acgccgcaaa gccattgacc gtggacgtga cattgttttg cgtatgtttg
     2641 gccaggttaa actggtgagt gaggagcctg ttttgctcga atttgccaaa tatacagggg
     2701 tgcagagcgt gagtgaattg tttttcgagg cctcacatgg gaaaattgac ataaaaatgg
     2761 tcaaggcctt tgccacagtt cacgatcgtt ctggctggct tcagtatctg cgcatgcctt
     2821 ttgtcagagg aaaatcgggc ataaaacccg gtacggagcg aagccgcttt attcgcaaat
     2881 caaaaggcgt tgagcttaac gttgactcca aaatctctta caccattgcc gattgctgca
     2941 atccaatacc gggcgacgaa gtggttggat ttgttgtctc ggaaaacacc ctcgaaattc
     3001 atcgggcaaa ttgcgcaaat gctgtgcgaa tcagcgccag atatggcgac cgcattaaaa
     3061 aagtacagtg gaacgattca aaactcatta cttttaaaac cgccattcag gtgtcgggaa
     3121 ttgataaaat cggaatattg agagatataa gccgcatcct ttccgaagaa aataatctga
     3181 atatcagctc catttttctt gaaagcaaag gcgaaacctt tgagggacgc attattcttt
     3241 ttgtcaacga taccgaacat ctcaaaaatc ttattgccca acttaaaaag gttgatggcg
     3301 ttcagcgtgt attccgattg cgtttgtctg aaaatcaatg atgttttgtt cattgaaatt
     3361 tattgggata gcatcttcct atttagggcc tgctgaaaaa gtctgaggcg cgccagattt
     3421 gaggcgacaa gacga
//
LOCUS       DAOPKE010000168         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_32127_length_3330_cov_12.982595, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000168 DAOPKE010000000
VERSION     DAOPKE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32127_length_3330_cov_12.982595"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKY63_12570"
     CDS             complement(<1..510)
                     /locus_tag="PKY63_12570"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPF01493.1"
                     /translation="MKALAVAIAQKLYYYYPELTIISSRHVGGGVISSAMEINTSDGH
                     FFVKFNPNNRFPGMFESEKKGLELLASAHTQLIIPEPILCDIDNGGTSFLLMQFLESD
                     IPEKHFWRNFGFRLALLHQNTSKQFGLKFNNYIGSLRQINTFHSNWTDFFIEERLQIQ
                     LNHCINNGYL"
     gene            581..1441
                     /locus_tag="PKY63_12575"
     CDS             581..1441
                     /locus_tag="PKY63_12575"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPF01494.1"
                     /translation="MKSLFLLFALLLSSFLMAQNIEKSNTKKHASNSAPADPKGYLIW
                     ENFEDLLMPPAFWQLQQGPTPETWDTATFDPSWGSAYVHCMYDESLAGVQNEYLITNV
                     MNMSTFANVSLNFYFQFSKYWGIAPYDNYDLIVLASTDSAQTFTDTLWTELSTDTSAW
                     NSFDWVFASVDLSAYIGEEKFALAFVYSGYDGAEAAIDMVSVETVGGMNENTISFNVY
                     PNPANEMLMIETFEKGLLSVCDLQGRSVMQKNIEGHEIMNVSTLPTGTYFVVFRTEMS
                     TKYIPLIITR"
     gene            1583..>3330
                     /locus_tag="PKY63_12580"
     CDS             1583..>3330
                     /locus_tag="PKY63_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF01495.1"
                     /translation="MKTIVFFVLMMPLFLLAQIRTNAVSQHKMLSAKNDNETGWRKIN
                     GIHYFPMLAKVNGSYSAEELPEGVLQGSRAGNIVSLYIRYDLLDAKINIKGLLYAEWA
                     RPVVPMLDRALPDARVDSVHQGINLPQPFTGKDVLIGITDWGFDYTNPNFYDTALQHT
                     RIEAAWDQWKTSGPAPSGFLYGTEFEGETELLSAQCDTSNVYGYAYHGSHVAGIAGGS
                     GAGTPYRGVAFEAGYLFTTFLVDAAAVLDAYSWMHQYAVNAGKRLVINQSWGLYYIGN
                     PDGTSLISEAIDSLSALGVVFVSSAGNNGDADFHLLYNASASDTLKTHVKFDSYSYYP
                     QMFGQCLSLWGTPGNSFEARIQVLNSSGVLLAETPFYLTDTLDFYVDDTLFVGADTVL
                     FNVLADSANLLNQRPFMQIRVASRNTANKVILQIHADSGQVHAWNLIELNNGVGNWGS
                     DFAAPYAGYTAGDPYYGIGEPACAHSAIAVAAHNSEYHHPTTGALLGGNIAYFSSYGP
                     LMDGTIKPEICAPGYNICSSVSSFTTTSFSNVVTSVSFGGRTYKFVRLSGTSMSGPLV
                     TGVVALMLDADSSLTV"
BASE COUNT          903 a          607 c          739 g         1081 t
ORIGIN      
        1 taaatatcca ttgtttatac aatgattcaa ttgaatttgt aatctttctt caatgaaaaa
       61 atctgtccag ttagaatgaa atgtattgat ttgccgcaat gatccgatat aattattgaa
      121 tttcagacca aattgcttcg atgtgttttg atgcaacagt gccagacgaa aaccaaaatt
      181 ccgccagaaa tgtttttctg gtatgtcgga ttcaagaaat tgcatcagca gaaaactggt
      241 tcctccattg tcgatatcac ataaaatggg ttcaggaatg attaattgag tatgagctga
      301 tgccagaagt tccaagccct ttttttcgga ttcaaacatg cctggaaaac gattattggg
      361 gttgaatttg acaaaaaaat gaccatctga tgtgttgatt tccatggccg aactgatgac
      421 tcctccgcca acatgccgcg aagaaataat tgttagctcc ggataataat aatataattt
      481 ttgcgctatt gcaactgcaa gtgctttcat atagcggatt ataaaatata tgaaatcaca
      541 aatataatta acttaccttt gcattgcaaa taatatcttt atgaagtctc tttttttact
      601 ttttgcattg ttgctttctt cgtttctgat ggctcagaat attgaaaagt caaatacgaa
      661 aaaacatgca tcaaattctg ctcctgctga tcccaaggga tatctgatct gggaaaattt
      721 tgaagatctg ttgatgcctc cggctttttg gcagctgcaa caaggaccta ctcccgaaac
      781 ctgggatact gcaacatttg atccttcctg gggaagcgct tatgttcatt gcatgtatga
      841 tgaatctctt gctggtgtgc aaaatgaata tctgatcacc aatgtgatga atatgagcac
      901 ttttgcaaat gtttcgctca atttctattt tcagttttcg aaatactggg gtatagcgcc
      961 ttatgataac tatgatttga ttgtgcttgc cagcaccgat agtgcacaga cttttaccga
     1021 taccctttgg acggaattaa gtaccgatac ttcagcatgg aatagttttg actgggtttt
     1081 tgcttctgtc gatctttcgg cttatattgg tgaagaaaaa tttgcgcttg cgtttgtgta
     1141 tagtggatat gatggcgctg aagctgcaat tgatatggtt tctgtcgaaa cggttggcgg
     1201 catgaatgaa aacactattt ctttcaatgt ttatccgaat ccggcaaatg aaatgctgat
     1261 gattgaaaca tttgaaaaag gtcttttatc ggtttgtgat ttgcagggta ggtcggtgat
     1321 gcagaaaaac attgaaggtc atgaaataat gaatgtttca acgcttccga caggaacata
     1381 ttttgtggtt ttcagaacgg aaatgtcaac aaaatatatt cccctgatca taacacgtta
     1441 gaaaatgttt ctgaatattc aatcagcccg cgtttgacgg gcttttttgt tttaaatggc
     1501 cgtcatgtcc aatttgggtt ttttaaggcg gaggacaatg gcggcttttt aaaaatatgt
     1561 acttttgaac aaatatcttt atatgaaaac cattgtattt tttgttttaa tgatgccttt
     1621 gtttcttttg gcacagatac gaaccaatgc tgtttcacaa cacaaaatgc tttcagccaa
     1681 aaatgacaat gaaaccggct ggcgtaaaat caatggcata cattattttc caatgctggc
     1741 aaaagttaat ggtagttatt ccgcagagga gctcccggaa ggggttttac aaggatcgag
     1801 agctggaaat attgtttcat tgtatattcg ttatgatttg cttgatgcaa aaataaatat
     1861 taaaggtttg ctttatgccg aatgggcccg tccggttgtt ccaatgctcg acagagcttt
     1921 gcccgatgcc cgggtcgata gtgtgcatca gggaatcaat ctgccacaac cgtttaccgg
     1981 aaaagatgta ttgattggta ttacggactg gggttttgat tataccaatc cgaattttta
     2041 cgatacggct ttgcagcata cacgtatcga agcggcctgg gatcaatgga aaacgtcggg
     2101 acctgcacca tcaggttttt tgtatgggac ggaatttgag ggagaaacag aattgctttc
     2161 tgctcaatgc gatacatcga atgtgtatgg atatgcatat catggaagtc atgtagccgg
     2221 cattgctggc ggtagcggag ctggaacacc ctatcggggc gttgcatttg aggccggtta
     2281 tttattcact acttttctgg tggatgcggc agctgttttg gatgcataca gttggatgca
     2341 tcaatatgca gtcaatgcag gaaaacgtct tgtaatcaat caaagctggg gtttgtatta
     2401 tataggaaat cctgatggga catctttgat cagtgaggcc attgattctc tttcggcttt
     2461 gggtgttgtt tttgtatcaa gtgccggaaa taatggtgat gctgattttc atttactata
     2521 taacgcgtct gcatcggata cattaaaaac tcatgtgaaa tttgacagtt attcctatta
     2581 tccgcaaatg tttggccaat gcctgtcttt gtggggaact cccggaaaca gtttcgaagc
     2641 acgtattcag gtattgaatt cttcgggtgt cttgttggct gaaactcctt tttatctgac
     2701 cgatacactt gatttttatg ttgatgatac tttgtttgtc ggagctgata cggtgttgtt
     2761 taatgttctt gccgattcgg ccaatctttt gaatcagcgt ccgtttatgc aaatcagggt
     2821 agccagccgc aatacagcca ataaggttat tcttcagatt catgctgata gtggtcaggt
     2881 tcatgcctgg aatctgattg aattaaacaa tggagtaggg aactggggtt ctgattttgc
     2941 ggctccctat gcgggttaca cggcaggtga tccatattac ggtataggtg aaccggcttg
     3001 tgcacattcc gcaatcgctg tggcagcgca taattccgag tatcatcatc caactacagg
     3061 tgcattgctt ggtggaaata ttgcctattt ttcgagctat ggtccgctga tggacggcac
     3121 catcaaacct gaaatctgtg ctccaggtta caatatctgt tcttctgttt catcttttac
     3181 aacaacgtca ttcagcaacg tggtaacatc tgtttcattt ggaggaagaa catacaaatt
     3241 tgtccgcctg agcggaacat ccatgtcggg accactggta acaggtgttg tggctttaat
     3301 gcttgatgcc gattcttctc tgacggtgaa
//
LOCUS       DAOPKE010000169         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_32432_length_3306_cov_15.620424, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000169 DAOPKE010000000
VERSION     DAOPKE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32432_length_3306_cov_15.620424"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKY63_12585"
     CDS             complement(<1..1036)
                     /locus_tag="PKY63_12585"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01496.1"
                     /translation="MNITRAITRKIQDKLTSGKVLVILGARRTGKTSLIREIIKQTDI
                     PYLMLNGEDFRVAAQLENKGIEEYRQIVGNKKLLIIDEAQKIKNIGKILKLMIDEIEG
                     LTILATGSSAFDISQKLGEPLTGRNISYNLFPIAQCELTETENKIETASNLEQRLIFG
                     TYPELLNLPDFVSKQEYLSELVNSYLLKDILELDSIKNASKILNILRLLAFQTGSEVS
                     FSEIAQKVALSRNTVERYIDLLEKTFIIFKVGGYSRNLRKEVTKNSKYYFVDNGIRNA
                     VISNFNTPELRNDIGQLWENYIISERVKYLSYNKTPHNSFFWRTYDQQEIDWIEERDG
                     KLFAYEIKYSR"
     assembly_gap    1680..1779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1780..2750
                     /locus_tag="PKY63_12590"
     CDS             <1780..2750
                     /locus_tag="PKY63_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPF01497.1"
                     /translation="KNGFALSPFFLPLKAGAMTARREPFHGNFGVFADSLPDGWGNLL
                     LDRVLRKYGLDPALLTTIQRLSLVGNNGMGALTYEPAFVVPEPQTLDDLNVIAAEVEM
                     IMNDQAYSETVEELYFQAGSSAGARPKVLIMYEGEHWLVKFASSMDPYNVGEIEFEYS
                     QRARRAGIEMPETRLFEGKYFGVKRFDREGDERFHMISAAGLLHADFRLPSLDYTELI
                     KATRALTGSVVEVEKMYRLMAFNVLIGNNDDHARNFSFIYKKGEWKCSPAYDLLPSVG
                     MNGEHCTAVAGNGRPNENDMLRVAAICSIENMRAKEIIEEVESSLL"
BASE COUNT          869 a          661 c          678 g          998 t
ORIGIN      
        1 acctgctgta tttgatttcg tatgcaaaca gcttcccatc tctctcttcg atccagtcaa
       61 tctcttgttg atcatacgtt ctccagaaaa aactgttgtg tggagtttta ttataggata
      121 aatatttaac gcgttcggaa ataatgtagt tttcccataa ttgtccaata tcattgcgaa
      181 gctcaggtgt gttgaaattt gagattacgg catttcggat tccattgtca acaaaatagt
      241 atttcgaatt ttttgtaact tcctttcgca gattacggct gtacccgccg actttgaaaa
      301 tgataaatgt tttttcaagc aaatcaatat atctttcaac ggtatttctg ctcaatgcca
      361 ctttctgcgc tatttcagaa aacgaaactt cactcccggt ctgaaatgca agcaacctga
      421 gaatattcaa tatcttcgat gcatttttaa tgctatcaag ctcaagtatg tcttttaata
      481 aatatgaatt gacaagttca gaaagatatt cctgcttcga aacaaaatcc ggcagattca
      541 gcaattcagg ataggtcccg aatattagtc gttgctccag attcgatgcc gtttcaattt
      601 tgttttctgt ttcagtcaac tcacattgcg ctattggaaa cagattgtaa ctgatgttcc
      661 ttcctgttaa tggttcgcct agtttctgcg aaatgtcaaa tgcagatgat ccggttgcaa
      721 gtatcgtcaa accttctatt tcgtcaatca tcagcttcag gatttttcca atgtttttta
      781 ttttttgcgc ctcatcaatg atcagtagtt ttttattgcc aactatttgt cggtattctt
      841 caattccttt gttttcaagt tgcgcggcaa cccggaaatc ttctccgttc agcatcagat
      901 atggaatatc ggtttgtttg atgatttctc tgatcaggct ggttttccct gtgcgtctgg
      961 cccccagaat caccaacact tttccggatg tcagtttgtc ctgaattttc ctcgtgattg
     1021 ctcgtgtaat attcattttc ctgttttaac tgcacaaata tacgaaaaaa cattgaatga
     1081 aatgaatgaa attagctaaa tacgttcaat ggctataaaa acacccttcc actaatacca
     1141 acttgaaata tacgatagac cataaagata tcatgtattt cacacattgg taaacgcagg
     1201 cgagaattat ttggacaaca atataattct cgccggtctt taagccttaa gcgttgctaa
     1261 attcagttgc ttaaagctta aaaatttaag gcttaagggt cagtgattca acgttgtctt
     1321 taagcattaa gcattaagca ttaaacatta agctgcgaag ctttgtgcgt tgtgccttgt
     1381 gcgtggagtt tatcacgctg caagcgtgac gttgagccgc atgcccggag tttatcccag
     1441 cgtccgccgc ggcggagcgg gaccgcccgc caaatacagc cttcgagcga ttatgatcaa
     1501 acctttaaat gaataaggtt tattttgctt tactgaaaaa aatgattatc tttgtttgtt
     1561 gtaaaaggta aaatattatc aattaagctg aaaaaacaaa ttaaagacta aaatattggt
     1621 cgaaatatcg gaaatattaa accgtaacaa tccaaacagc attgccatgg aactggcaan
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaagaatggt tttgcgcttt
     1801 caccattttt tctcccgctg aaggccggcg ccatgactgc ccggcgtgag ccattccatg
     1861 gtaacttcgg cgttttcgcc gacagtcttc cggatggatg gggaaatctt ttgttggatc
     1921 gtgtattgcg aaaatacgga ctggatccgg cattgctcac gaccattcag cggctttcgc
     1981 ttgttggaaa taacggcatg ggtgcattga cctatgaacc cgcatttgta gtacctgagc
     2041 cgcagacatt ggatgatctc aacgtaattg ctgctgaagt tgagatgata atgaatgacc
     2101 aggcatatag cgaaactgtc gaagagttgt attttcaggc aggttcatct gctggtgcca
     2161 gacccaaggt gctgattatg tacgaaggtg agcactggct ggtgaaattc gcttcatcaa
     2221 tggatccgta caatgttggt gaaattgaat tcgaatactc gcagcgcgca cgcagagccg
     2281 gaattgaaat gcctgaaacc cggctttttg aaggaaagta ttttggcgtg aaacgtttcg
     2341 accgtgaagg tgatgagagg tttcatatga tatcggctgc gggattgctg catgccgatt
     2401 tccggttgcc gtcgctggat tataccgagt tgattaaagc taccagagcg ctgaccggga
     2461 gtgttgtaga ggtcgagaaa atgtaccggc ttatggcttt caatgtcctg attggaaata
     2521 atgatgatca cgcgcgcaac ttttctttta tttacaaaaa aggtgaatgg aaatgctcac
     2581 ctgcatatga tttacttcct tcggttggaa tgaatggcga acactgcaca gctgttgcgg
     2641 gcaacggacg tcccaatgaa aacgatatgc tgcgggttgc agctatctgt agtattgaga
     2701 atatgcgggc caaagagatt attgaggaag ttgaaagttc tttgttgtga cgcacaaagt
     2761 gtatcgctgg ttaatacgca aatagcataa tacggaatta gggtctgctg aaaaacacgt
     2821 aacactcaca atattagaaa catatttatc acattggact ttgcaaatgc aagtgttttt
     2881 gtccggttag cgaaataacc ttgcattaga gaatctgctg ctgcagtttt gaaatcatac
     2941 aaatcatttt ttatttttcg cccatcattt caaaataact tcaactatta ctgtgagtgt
     3001 ttgttagcct gaggcgcgcc atcttcttcg ttgccatcga ctcgaagtat tactatacat
     3061 cttcgtcttg tcgcctcgaa tacttgtgcc gtacgaagtt acggtatagc gcacctcaga
     3121 ctttttcagc aggcccgaat tacacaaaac cttaagcttt atgcattaag ccttaagctt
     3181 taagcattaa gccttatgct gcctgcgttg agctctgcgc attgtacgtc tgaaagtccc
     3241 gaccataagc gccgggatgc gccctgcgcg ttgcgcctca tgcgttgtgc cctgtgcgtt
     3301 gtgccc
//
LOCUS       DAOPKE010000170         3293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_32602_length_3293_cov_11.330142, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000170 DAOPKE010000000
VERSION     DAOPKE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32602_length_3293_cov_11.330142"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..449
                     /locus_tag="PKY63_12595"
     CDS             174..449
                     /locus_tag="PKY63_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPF01498.1"
                     /translation="MGKGDMKSKRGKIHRGSYGKKRPRHAEKLAPVAKELEEKKKTAK
                     PKEKAVKPVEAVAEEPKAKKAPAKAKAKKEATDQPDLFDAGDAAKTE"
     gene            617..>1538
                     /locus_tag="PKY63_12600"
     CDS             617..>1538
                     /locus_tag="PKY63_12600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF01499.1"
                     /translation="MICPFCETAKPDVAYTASGFHFFRCPACHSLFRADSDVAIDHQY
                     DEDYYGDSPKEKFWLKPVVWLLNAERGSRAKFISKKIPKDSSVLDIGCGNGALLHKIH
                     QLSGAAVTGVEMDNVAARRARDRGCITVYSDSFEKAPLTEGSFDAVSMIHSFEHIPQP
                     APTLTKAVALLKPGGTFYMAIPNINSIQYSIFRKHWLHLDPEFHLHFVSRQQLLKLAS
                     GTGLKHIKTRHFNPVQNIPSFILSAMNLVTSRRDVLFNMLRHKKKLRNPFNILLFIFL
                     MLFAAILLPFAIIEECISCLTGCGATVDYVF"
     assembly_gap    1539..1638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1746..1991
                     /locus_tag="PKY63_12605"
     CDS             1746..1991
                     /locus_tag="PKY63_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF01500.1"
                     /translation="MENLSLSDFIKNRRKKLKLTQSQIAAKAGVGLRFIRELEQNKTT
                     LRMDKVNQVLALFGMELGPQPINRKNLSDEESRSQNA"
     gene            1963..2286
                     /locus_tag="PKY63_12610"
     CDS             1963..2286
                     /locus_tag="PKY63_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPF01501.1"
                     /translation="MRKAEVRMHDIPAGILTEDDSGYTFTYLPLYLSKPEFPAISLTL
                     PKTDIPYKSNVLFPFFDGLIPEGWLLDIAQKNWKINQRDRMGLLMVCCLDCIGAVSIV
                     PLIEE"
     gene            2288..3241
                     /locus_tag="PKY63_12615"
     CDS             2288..3241
                     /locus_tag="PKY63_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPF01502.1"
                     /translation="MKRCLYCYKELGDEVGDYHFACSKVYFGKPVAPEFSYVEKDLPE
                     LGLQVIRSHYAVTGVQPKISLSLQKGENKDAPMRLTIVGFDSKYILKPQTDLYPHLPE
                     VEDLSMHLAAIAGISTVPHTLIRTKSNNLAYITTRIDRLKKARIHMEDMCQLTERLTE
                     HKYHGSHEQIGKTISKYSQNPGLDLVNFYEQVLFSFLIGNADMHLKNFSLLADPDLGY
                     TLAPAYDLVSTALVLPSDTEDLALTLNGKKKKLRLSDFMKAMTSNGIPEKAQRNIVDK
                     MEAAVPEIIQFIDYGFLPKTMKELFQKIISDRAHRLYFKKV"
BASE COUNT          958 a          670 c          690 g          875 t
ORIGIN      
        1 ttaacctcaa ccttaacctc aaccttaacc tcaaccttaa cctcaacctt aacctcaacc
       61 tcaaccttaa ccttttttgg gctagctaca gaatgtcagg gaagcaccta tagatgaaat
      121 aaagttttgc atattaaaaa tattcccgta cgtttgtaaa aaaatataca gttatgggaa
      181 aaggagatat gaaatcaaaa cgcggtaaaa ttcaccgtgg cagctatggt aaaaaacgtc
      241 cgcgtcatgc agaaaagctt gctccggttg caaaagaact cgaagaaaag aaaaaaactg
      301 caaagccgaa agaaaaagcg gttaagccag tagaggcagt tgccgaagaa cccaaagcaa
      361 aaaaagcgcc ggccaaagcc aaagcgaaaa aagaagctac cgatcagccc gatttgtttg
      421 atgccggcga tgctgcgaaa acagaatagc taacgcaaaa gttacgataa ttaatctgtt
      481 gactctttga cggcctaatc ctgctgtctc cttcgatact tttcttcgct tcgcttcaaa
      541 aaactcagga tgacagcagg actcggatga aagtaaattc attatgacaa aagtggggtc
      601 aactatttac ccaaatatga tttgtccttt ttgcgaaact gcaaagccgg atgttgctta
      661 taccgcttcc ggctttcact ttttcagatg tcccgcatgt cattcgcttt tccgcgccga
      721 cagcgatgtt gcgattgatc accaatacga cgaagactat tatggtgaca gcccgaaaga
      781 aaaattctgg ctgaaacctg tcgtttggct gctcaatgca gagcggggca gtcgcgcaaa
      841 atttatttca aaaaaaatcc cgaaagattc atctgtgctc gacattggct gcggcaatgg
      901 tgcgctgctg cataaaattc atcagttgtc aggtgcagca gttaccggtg tcgaaatgga
      961 caatgtcgcg gcacgccggg cgcgtgaccg tggctgtatc accgtgtatt ccgattcatt
     1021 cgaaaaggcg cccctaaccg aaggcagttt cgatgcggtt tccatgattc attcgtttga
     1081 gcatattcca caacccgcgc ccacactgac aaaagcagtt gccttactca aacccggtgg
     1141 cactttttac atggccattc caaacatcaa ttccattcaa tattctatat tccggaaaca
     1201 ctggctgcat ctcgatccgg agtttcatct gcattttgtt tcgcgtcagc aattgctgaa
     1261 acttgcatct ggaaccggtt taaagcacat taaaacccgc cacttcaatc cggtgcaaaa
     1321 tattccgagc tttattctta gcgccatgaa tcttgtaacc agccgtcgtg atgtgctttt
     1381 caacatgctg cgccacaaaa agaaattacg caatccattc aacattttat tgtttatttt
     1441 tctgatgcta ttcgccgcga ttttgctgcc ctttgccatc attgaagaat gcatttcgtg
     1501 cctcaccggc tgcggcgcca cggtggatta tgtgtttgnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnaa tattacaccc gatagggtac aataatgtta attattggcg
     1681 aaatattacc cgttcgggta taaatttcct gttttgttgt atatttgtac cctaacgggt
     1741 ataaaatgga gaatctttca ttatcggatt ttataaagaa cagaagaaaa aagctgaagc
     1801 tcacgcaaag tcagatagct gcaaaagcgg gtgtaggttt gcgctttata agggaattgg
     1861 agcaaaacaa aaccactttg cggatggaca aagtaaatca ggttctggcg ctttttggaa
     1921 tggagctggg accacaacca attaaccgaa aaaatttaag cgatgaggaa agcagaagtc
     1981 agaatgcatg atataccggc cggaatcctg actgaagatg acagtgggta cacttttact
     2041 tatttgccat tgtatctttc aaaaccagaa tttccggcaa taagtctcac attaccgaaa
     2101 acagatattc catacaaaag caatgtatta ttcccgtttt ttgatgggtt gattccggaa
     2161 gggtggttgc ttgatattgc tcagaaaaac tggaaaatta atcaacgtga ccgcatgggg
     2221 ctgttgatgg tttgttgttt agattgtatt ggagctgtaa gtattgttcc tttaatcgaa
     2281 gaatgaaatg aagcgttgtc tgtattgtta taaagaactt ggcgacgaag tgggtgatta
     2341 tcactttgca tgcagcaagg tatatttcgg caagcctgta gcacctgagt tttcttatgt
     2401 agaaaaggat ttaccggagc tgggtttgca ggtcatacga agtcattatg ccgtgactgg
     2461 cgtacaaccg aaaatatcgc tgagcttaca aaaaggagag aacaaagatg caccaatgcg
     2521 tcttactata gtaggctttg atagtaaata tattttgaag ccccaaaccg atttatatcc
     2581 gcacttgccg gaagtggagg atttatcaat gcatctggct gcaatagccg gaatttccac
     2641 agtgcctcat acgttaatcc gaacgaagtc caacaacctg gcgtacataa caacacggat
     2701 tgaccggctg aagaaagccc ggatacacat ggaagatatg tgtcaattga ctgagcgtct
     2761 gaccgagcac aaatatcacg gatcgcatga gcagattgga aaaacgatct caaagtattc
     2821 ccagaaccct ggtctcgatc ttgtgaattt ttatgagcag gtgctttttt catttctgat
     2881 tggaaatgca gatatgcatc tcaagaattt ctcattgttg gctgatcctg atcttggata
     2941 tacgctggct ccggcttatg atcttgtttc tacggcattg gtattaccgt ctgataccga
     3001 agatttagcg cttactttaa acgggaaaaa gaaaaagtta cggctaagtg atttcatgaa
     3061 agcaatgacg agtaatggta tcccagaaaa agcacagcga aacattgttg ataaaatgga
     3121 ggctgctgtt cctgaaataa ttcagtttat tgactatggt ttccttccga aaacgatgaa
     3181 agaattgttt cagaaaatta tctcagaccg ggcacacaga ctgtatttca aaaaagtctg
     3241 atgcatcatc ggcgtagttt caatagcaat cggggttcgt atttgttcgc cca
//
LOCUS       DAOPKE010000171         3200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_33890_length_3200_cov_17.478855, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000171 DAOPKE010000000
VERSION     DAOPKE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33890_length_3200_cov_17.478855"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..1088)
                     /locus_tag="PKY63_12620"
     CDS             complement(429..1088)
                     /locus_tag="PKY63_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF01503.1"
                     /translation="MTDKIKIMLVDDHDIVRDGVNAIIAGTTDIEVVAEAGSVAEALN
                     ILEQCVPDVLILDISLPDKSGIDAIPLVHQIRSSLPVLILSMHNNEEYIIRAIKAGAR
                     GYLPKNIPARELTDAIRRIFTGEEYFGEATSVVLRKALLRKLEKEEKADPASEPLSQR
                     ENEVLQLFCAGLSNQEMADKLFISIRTVETHKTHIMQKLGLKTTVELIKYGIRNKIIE
                     I"
     gene            complement(1095..2894)
                     /locus_tag="PKY63_12625"
     CDS             complement(1095..2894)
                     /locus_tag="PKY63_12625"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPF01504.1"
                     /translation="MKTNLRDRFVLFLSLTGIIIIAVTGLLTYRIARESVTDRTFAQL
                     KTIRNARKFQLEQYFRDRSAEVLQLGRSQELNNVFSELNNKSGQLHDGDSIGISSPGS
                     YVFSAVFDRPGHHELILVSDKSSGFIISPDSHKQTAAIYFSQKQNAGFLKAAELSIRE
                     KDVIITDYDTATGLPLIHVFAPLQLDSNISGAVGISISADALNSILLEQNDQNGLGYS
                     GEVYVTGNDYLMRSQSRFQEQSVMKTLVKTNPTADVFASNEGQMLGNDYRGIPVLSSY
                     SRLQIPGLDWAILAEIDKREALLPLENLRNTLLVVSVAIVFVLVFVSFWLSNFFTKPI
                     LRLRNAVSEIGKGRLGIKVEHQSSDEIGELADAFNEMSQKLSDQHNEILAREKQLDEE
                     RKQRFESFIEGQEKERQRLSRELHDGIGQIFVAARFQLESMGQYENIQNLAPYVASQK
                     LVNQGITELRKISKGLVPSELSEFGLSSALRSLCTQMEESAGIRIQQAITDVDLTLSE
                     LNATHLYRILQEALTNAVKHSNASEIIVEFVFENNELSLQIQDNGCGFGPEEPMRGKG
                     LANIRERAALLDAKLNINSQQNCGTRIELTYQV"
BASE COUNT          802 a          737 c          699 g          962 t
ORIGIN      
        1 tgtagaccat ataaaacaaa accacgactc cggaggagtc gcatataagg agctttgtct
       61 tctgacaatg cttgtttaaa tgcaatccgc cagccggcgg atcgtgtggt cggtattggt
      121 ttttctacag atatgcgatg cctgcctgtt tgtcaggcat cagccaacaa aacgttcaat
      181 gaaattgcta caattgacta ttttatattt ccatttggta taaaaagtac aaaaacagga
      241 tccgccagct ggcggatcca atccacctgt ttttgaaaaa cattgtttca atattttcgt
      301 tttattcgac ctctccgagg tcgttcttgt tctacctttt acccggccat actggccggg
      361 ctattattat ttgacccgcg ctgcaggtcg gttaatacca aagtgtcctg atggcgaaat
      421 caggaaaatc agatttcgat tattttattc cggatgccat attttatcag ctccaccgtt
      481 gttttaagcc cgagcttctg cataatatgc gttttgtgtg tttcaacagt gcggatgctt
      541 atgaacagtt tgtcggccat ttcctgattc gaaagacctg cacaaaacag ctgcaacact
      601 tcgttttcgc gctgcgacaa tggttctgat gccgggtccg ctttttcttc tttttccagt
      661 ttgcgcaaca gcgctttgcg cagcaccacc gacgtcgctt cgccaaaata ttcttcacca
      721 gtgaaaatgc ggcgtatggc atcggtgagt tcccgtgccg gaatattttt cggcagatat
      781 cctcttgcac cggcctttat tgctcggata atatattctt cgttattgtg catggacaga
      841 atcagcaccg gcaacgaaga cctgatctgg tgaaccaaag gaatggcatc aatgcccgac
      901 ttgtcgggaa gcgaaatgtc gagaatcaga acatccggca cgcattgttc cagaatgttc
      961 aatgcttctg caacagaacc ggcttctgca acaacttcaa tgtcggttgt tcctgcaatg
     1021 attgcattca cgccatcacg tacaatgtcg tgatcatcca caagcattat ttttattttg
     1081 tctgtcatgt ttttttatac ttgataagtc agttctattc tggttccgca attctgctgt
     1141 gaattgatat taagttttgc atccagcaat gcagcccttt cccgtatatt ggcgagtccc
     1201 tttccacgca tgggttcttc cgggccaaat ccacagccat tgtcctgtat ctgcaacgaa
     1261 agctcattgt tttcgaaaac aaattcaaca ataatttctg aagcattgct gtgtttaaca
     1321 gcattggtca gtgcttcctg cagtattctg tacagatggg ttgcattcag ttcgcttagc
     1381 gttaaatcaa catcggtaat ggcctgttga attcgtattc cggcactctc ttccatttgt
     1441 gtgcagagac tgcgtaaggc gctgctgagg ccaaattctg aaagttcaga aggaacaagt
     1501 ccttttgata ttttccgcaa ttcagtgatt ccctgattga ctaatttttg cgaagccacg
     1561 tatggagcca gattctgaat gttttcatat tggcccatgc tttcaagctg aaaacgtgcg
     1621 gccacaaaaa tctgtccgat gccatcgtgc agttctctcg aaagtcgctg ccgttctttc
     1681 tcctggcctt caataaagga ttcaaaacgt tgttttcgtt cttcgtcgag ttgtttttcg
     1741 cgcgccagaa tttcattgtg ctgatcgctg agtttctgcg acatttcgtt gaaggcgtcg
     1801 gccagttcgc caatttcatc actgctctga tgttcaacct taatgcccag gcgccctttg
     1861 ccgatttcgg aaactgcgtt gcgcagccgg agaatgggtt ttgtaaaaaa gtttgaaagc
     1921 cagaaggaga caaaaacaag aacaaataca atggcgacac ttacaaccaa caatgtgttt
     1981 cgcagatttt ccaatggaag cagggcttcg cgtttatcaa tttcagccag aatggcccag
     2041 tccagcccgg gtatctgcag ccggctgtag gagctgagaa ccggaattcc gcgataatca
     2101 ttgccaagca tttgtccttc gttgctggca aaaacatcgg cagtggggtt tgttttcacc
     2161 aatgtcttca tgacggattg ctcctgaaaa cgtgactgac tccgcatcag ataatcgtta
     2221 ccggtcacat aaacttcgcc gctgtagcca aggccgttct gatcattctg ttccagcaga
     2281 atactgttca gtgcatcggc cgaaatgctg attccaacag cgccggaaat atttgaatcc
     2341 agctgcagcg gtgcaaaaac atgaatcaaa ggaagtcctg ttgcggtatc ataatctgta
     2401 ataatgacat ccttttcccg aatggacaat tctgccgcct tcaaaaaacc ggcgttttgt
     2461 ttttgcgaaa aatatatggc tgctgtttgt ttgtgactgt caggtgaaat tatgaagccg
     2521 cttgatttgt ctgaaacaag tatcagctca tgatgtccgg gacggtcgaa cacggctgaa
     2581 aacacatagc ttccgggtga tgaaattccg atggaatcac cgtcgtgcaa ttgacctgat
     2641 ttattgttca gctctgaaaa aacattgttc agttcctgtg aacggcctaa ctgcaacacc
     2701 tctgcactgc ggtcgcgaaa gtattgttca agctgaaatt tgcgggcatt gcggattgtt
     2761 ttaagctgcg caaatgtccg gtctgttacc gattcccgcg caatgcgata agtgagcagt
     2821 ccggtcacgg caatgatgat aattcccgtc agagacagaa ataaaacaaa gcggtcgcgg
     2881 aggttggttt tcattttgaa tcaggtggta caaatatacg ggttctgtat ttgcctgcaa
     2941 catccatctt tatattggga atttctctaa attgatacgc gttacgatat tccatcgttc
     3001 cggccaaaat taaccctcga agagttccgg actcttcgag ggttttataa acaggccgga
     3061 atatggaatc gtcgtaacag attcaaaaac aacgattcca tatcctttcg ctcctacgtc
     3121 gctttcggac gatggaatat tgtttttgaa gaagtcttgt ttttggacat cctctttgtt
     3181 tttctatttt ctaaagacat
//
LOCUS       DAOPKE010000172         2944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_38137_length_2944_cov_8.627207, whole genome shotgun sequence.
ACCESSION   DAOPKE010000172 DAOPKE010000000
VERSION     DAOPKE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38137_length_2944_cov_8.627207"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(550..623)
                     /locus_tag="PKY63_12630"
     tRNA            complement(550..623)
                     /locus_tag="PKY63_12630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(587..589),aa:Met,seq:cat)
     gene            693..1439
                     /locus_tag="PKY63_12635"
     CDS             693..1439
                     /locus_tag="PKY63_12635"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPF01505.1"
                     /translation="MRTIHTMLIIMACFCASGQVLNIEQQRIITDTIGWSGTVGFSSS
                     LTKNSSTLLSVNMKDHVQYKTDTALYLMFLDYGLTKSKESDFDNAGTLHFRYNYKIKP
                     YLTFEAFTQGQFNKLLNVQFRWLSGCGPRFKVIGKKHFRVYAAALYMYEFEEVESPRL
                     FHRDHRLSNYISATWKFKSSAIVTNTLYYQPLISSFADYRIFNQFDLNFKITKNLSFT
                     TSWRYFFDSNPPENVVHSTQFLSNEITYQF"
     gene            complement(1448..2902)
                     /locus_tag="PKY63_12640"
     CDS             complement(1448..2902)
                     /locus_tag="PKY63_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPF01506.1"
                     /translation="MKTFEEIRKNIVGDNAILKTPYGEFPLIYADWIASGRMYKPIEE
                     RISDVIGPWVGNTHTETSATGTLMTHAYHHARDIIKNHVNAGPGDVFLPVGFGMTAAI
                     NKLQRILGLKYCGKINSSNDCLDTSIRPVVLISHMEHHSNHTSWYETAADVVVVPPGN
                     DMLIDLNHLETALKKYQGRRIIGSFSAGSNVTGIETPIHEMAALVHKYGGICFADYAA
                     TAPYSDINMHPGPEGQDLDGVFFSPHKFLGGPGSSGVLIFNGKLYSNKAPDNPGGGTV
                     EWTNPWGEYRYIDDIEVREDGGTPGFLQTMRTALSIKLKEQMGTKNIHAAEDMLLKRA
                     FAAFDQMDGIHVLAPEHRKRIGVISFYHEKMHYNLIVKLLNDRFGIQVRGGCACAGTY
                     GHYLLDVSYEKSQHITDMINHGDLSLKPGWVRLSLHQTMTIQDIDYICNALNEVIANH
                     ESWGKDYTYDSHKNDYIHKSGKSTFTDVTKWFEL"
BASE COUNT          783 a          677 c          620 g          864 t
ORIGIN      
        1 atatatgagt tatagcgaat ggctgatctt cgctctcgct ctgattctcg ttctggttcc
       61 ctgcatcgag tgtgagaacg ggagcgaagt aaaaaacaga actccaaacc tcgctctcgc
      121 tctgacttaa gtgtgagtgc gagtgaacag aaaaaaaaca gccctttggg ctgttttttt
      181 ttgtagcggg gacgagagtt gaactcgtgt ccgccagctg gcggatatga atccgtcgct
      241 catttaatgc cagccttctg ctcttttatt aaatcccaga taaaatctct tcctttggcc
      301 gatttgagct gcttctctct atccattgca gaccttttat cctgatattc ctctgtgtaa
      361 aataataacc acggcctata cctaattgtc cagcctttat ttgcaaggac attatgagat
      421 ttaagtctgg ctgttacatc tgaagaatac ccaatgtaaa tcttgtcaaa ttcagccgaa
      481 tataaaacat aaactgtata catcttgaaa acatctacaa aaaaaacagc ccaaaagggc
      541 tgttttttgt agcggggacg agagttgaac tcgtgaccta cgggttatga atccgtcgct
      601 ctaaccacct gagctacccc gccatttggg agtgcaaaag taataaatca ttctatttcg
      661 gacaaaaata tttactttga acaaaaaatt ttatgagaac aatacacaca atgcttatca
      721 taatggcgtg tttttgcgca tcaggacagg ttttgaacat tgagcagcaa cgtattatca
      781 ccgatactat cggctggtcc gggaccgttg ggttttcgtc gtcgttaaca aaaaacagct
      841 caaccctgct ttcggtcaac atgaaagatc atgttcagta taagaccgat actgcgttat
      901 atctcatgtt tctcgattac ggacttacca aaagcaaaga atcggacttc gataatgccg
      961 gaaccctgca ctttcgttat aactataaaa taaagccata tttgacgttt gaagcattta
     1021 ctcaaggtca gttcaacaaa ctgctcaatg tgcaattcag atggctcagc ggctgcggac
     1081 cgcgttttaa ggtcatcggg aaaaagcatt ttcgggtgta tgctgccgcg ttatatatgt
     1141 atgaatttga agaagttgaa tctccccgcc tgtttcatcg tgatcatcgc ttaagtaact
     1201 atatttcagc tacctggaag tttaaatcca gcgccatcgt caccaacacc ttgtattatc
     1261 agccattaat aagttccttt gccgattatc gtatattcaa tcagttcgac ctaaacttta
     1321 aaatcaccaa aaatctgtcg tttactactt cgtggcgcta cttcttcgac tccaatccac
     1381 ccgaaaatgt ggtgcacagc acccaatttc taagtaacga aatcacctat caattttaat
     1441 tgattgctta cagctcaaac catttagtta catccgtaaa cgtacttttt ccgcttttgt
     1501 gaatgtagtc attcttgtgt gaatcgtagg tgtagtcttt accccatgat tcgtgatttg
     1561 ctataacttc gttcagtgcg ttacaaatgt aatcaatatc ctgaatggtc attgtctggt
     1621 gaagcgacag tctcacccac cccggtttga gcgaaagatc gccatggttg atcatgtcgg
     1681 taatgtgttg cgatttctca taactcacat cgagcagata gtgtccgtaa gtaccggcac
     1741 aagcacaccc acccctgacc tgaatgccaa accggtcatt tagcagctta acaatcagat
     1801 tgtaatgcat tttttcgtga taaaaagaaa tcaccccgat tcttttccga tgttcgggtg
     1861 caagaacatg aattccatcc atctggtcaa aggccgcaaa agcacgtttt agcagcatat
     1921 cttcggctgc atgtatattt ttcgtcccca tctgctcttt cagtttgatg gagagtgcag
     1981 tgcgcatggt ttgcagaaag ccgggtgtac cgccgtcttc gcgcacttct atatcgtcga
     2041 tgtaacggta ttcgccccat ggattggtcc attccacggt accgccgccc ggattgtctg
     2101 gggctttgtt gctgtagagt tttccattga aaatcaatac cccgctcgat ccaggaccgc
     2161 caagaaattt atgtggacta aagaaaacac cgtcaagatc ctgaccttcg ggacccggat
     2221 gcatgttgat atcgctgtag ggcgctgtgg ctgcataatc ggcaaaacat attccaccgt
     2281 atttgtgaac caatgctgcc atttcgtgaa taggtgtttc gattccggtt acattcgatc
     2341 cggccgaaaa cgaaccaatg atgcgtcgtc cctgatattt ttttaatgct gtttccagat
     2401 gattcaaatc aatcaacatg tcgttgccgg ggggcactac cactacatct gctgcggttt
     2461 cgtaccaact ggtatggttg gaatgatgct ccatatgact gataagtacg accggacgaa
     2521 tgctagtgtc gaggcaatca ttgctgctgt ttattttacc gcagtatttc agccccagaa
     2581 tgcgctgaag tttgtttatg gcagcggtca ttccaaaccc cacgggcaga aaaacatcgc
     2641 caggacctgc atttacatgg ttttttataa tgtcgcgggc atgatgatag gcatgggtca
     2701 tcagcgtgcc ggtagcgctg gtttcggtat gggtattccc tacccaaggc ccgataacgt
     2761 ctgaaatccg ctcttcgatg ggcttgtaca tgcgtccgga ggcaatccag tctgcataaa
     2821 tgagtggaaa ttctccgtag ggggttttca gaatggcatt gtcgccaacg atatttttgc
     2881 ggatttcttc aaatgtcttc atcgggggaa tagatttcag cgcaaaagta aaaaatactt
     2941 ccta
//
LOCUS       DAOPKE010000173         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_38310_length_2935_cov_15.607986, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000173 DAOPKE010000000
VERSION     DAOPKE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38310_length_2935_cov_15.607986"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..964)
                     /gene="tsf"
                     /locus_tag="PKY63_12645"
     CDS             complement(131..964)
                     /gene="tsf"
                     /locus_tag="PKY63_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPF01507.1"
                     /translation="MANISAQDVYKLRDLTGAGVMDCKNALVESEGDFEKAIEILRKK
                     GQKLAVKRADREANEGFVIAATDETNTFGAVIMMNCETDFVAKNDDFVSATKSFIDAA
                     IAFKPKNLDALNALQIDGRSIADLTTDLVGKIGEKITLTHYECIEAPCVVVYNHYNHR
                     ISTIAGLTESGEKALEAGKDVTMQIASMKPIAVDKDDVSAEIIAKEIEIGKEQARQEG
                     KPEEMLEKIAQGKLNKFYKESTLLNQDYIKDGSMTVAQYLKSVSANLNVTKFYRLALG
                     E"
     gene            complement(978..1844)
                     /gene="rpsB"
                     /locus_tag="PKY63_12650"
     CDS             complement(978..1844)
                     /gene="rpsB"
                     /locus_tag="PKY63_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPF01508.1"
                     /translation="MANVNFDQLLEAGCHFGHLKRKWNPNMAPYIFMEQNGIHIIDLY
                     KTIAKMDEAGAALKQIAKSGRKILFVGTKKQAKEIVAESAKNASMPYVVERWPGGMLT
                     NFATIRKAVKKMAYIDKMLNDPSFTNISKRERLQIKREREKLEKNLGSIAELNKLPSA
                     LFIVDIAKEHIAVDEARILGIPTFAIVDTNSDPKKVDFPIPGNDDAGKSISLILQEVV
                     SHINEGLEERKMTKDAEVSEEAEEEYDKDGNLIEPALDEEDGSDKPKRKRVLSKAPAK
                     GGHRKTNIEVKD"
     gene            complement(1953..2339)
                     /gene="rpsI"
                     /locus_tag="PKY63_12655"
     CDS             complement(1953..2339)
                     /gene="rpsI"
                     /locus_tag="PKY63_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPF01509.1"
                     /translation="MSETLTLGRRKTAVARLRLTPGNGTMTVNGRDYKNYFPLETLQY
                     IIDQPFIITGTTGKYDVKALLDGGGYKGQAEALRLAISRALVELNEEYRPALKEQGLM
                     RRDPRMVERKKPGRPKARKRFQFSKR"
     gene            complement(2345..2800)
                     /gene="rplM"
                     /locus_tag="PKY63_12660"
     CDS             complement(2345..2800)
                     /gene="rplM"
                     /locus_tag="PKY63_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPF01510.1"
                     /translation="MDTLSYKTKSINKANANKEWVIVDATDQSLGRFAAMVAFMLRGK
                     HKPSFTPNSDCGDNVIVINAEKVKLTGNKLTDKEYVRHTGYPGGQRFTNPADLLKRKP
                     EAVVEMAVKGMLPKTKLGAELFRNLYVYAGTEHKHEGQQPKSVNINEIK"
BASE COUNT          674 a          608 c          650 g         1003 t
ORIGIN      
        1 agcatccagc tcatgcggtc atcgacaagt gcaaatattt ttttcaggaa gacattggtt
       61 tttgcggagt caaatataaa caaaaaagcc acccggagag gatggctttt caataatgtg
      121 tctgatgcga ttattcgccc aatgcaaggc ggtagaattt ggtcacattc aggttggcac
      181 ttactgattt caggtactga gcaacagtca tgctgccgtc tttgatataa tcctgattta
      241 gcagagtact ttccttatag aatttgttga gtttgccttg ggcaattttc tcaagcattt
      301 cttcgggttt accttcctgg cgtgcttgtt ctttgccaat ttcgatttcc tttgcaatga
      361 tttcggccga aacatcgtcc ttgtcaacag cgatgggttt catcgaggca atttgcatag
      421 ttacatcttt gccggcttca agcgcttttt cgccactttc ggtcaggccg gcgatagtgc
      481 tgatgcggtg attgtaatga ttatagacta caacgcaagg agcttcgatg cattcgtagt
      541 gggtaagggt aattttctcg ccgatttttc caacgaggtc agtggtgagg tcagcaatgc
      601 tacggccgtc aatctgaagt gcattgagtg catccagatt tttgggttta aaagcgatcg
      661 ctgcatcaat gaatgatttg gtggctgaaa cgaagtcgtc gtttttggca acgaaatcgg
      721 tttcacaatt catcataatc actgcaccga aagtgttggt ttcgtcggtt gctgcaataa
      781 caaaaccttc gtttgcttcg cggtcagcac gttttacggc cagtttttgt ccttttttac
      841 gaagaatttc aatggctttt tcgaaatcgc cttctgattc aacgagggcg tttttacaat
      901 ccatcacacc ggctccggtc aaatcgcgga gtttatatac atcttgtgca ctaatatttg
      961 ccatggttct gaaattttta gtcttttact tcaatattgg ttttgcggtg accaccttta
     1021 gcgggggcct tgctcaatac gcgtttgcgt tttggtttgt cggatccgtc ttcttcgtcc
     1081 agagcgggtt ctataaggtt tccatccttg tcgtattctt cttccgcttc ttcggatact
     1141 tcagcatctt tggtcatttt tctttcttcg agaccttcgt tgatgtggct aacaacttcc
     1201 tgaagaatca gtgaaattga tttgccggcg tcgtcgtttc cgggaatcgg aaaatcaacc
     1261 tttttgggat ctgaatttgt atcaacgatt gcaaaagtcg ggatgcccag aatccgggct
     1321 tcgtcaacag caatgtgttc tttggcaata tcaacaatga aaagtgctga aggaagtttg
     1381 ttcagttcgg cgattgaacc aaggttcttt tcaagttttt cacgttcacg tttaatttga
     1441 agacgttcac gttttgaaat attggtgaaa gaagggtcgt tgagcatttt gtcaatatag
     1501 gccatttttt tcacagcttt gcgaatggtg gcgaagttgg tgagcatgcc gcccggccag
     1561 cgttctacca cataaggcat cgatgcgttt ttggctgatt cagccacgat ttcttttgcc
     1621 tgtttttttg ttcctacaaa gagaattttt cgtcctgatt ttgcaatttg tttcagagct
     1681 gcaccggctt catccatttt ggcaatggtt ttatacaagt cgatgatgtg aattccattt
     1741 tgctccatga aaatataagg agccatgttg ggattccatt tgcgtttgag gtgaccgaag
     1801 tgacatccgg cctccagaag ttgatcaaaa tttacgtttg ccatttaaat tgtgttattt
     1861 gtttacattc ctttcgtttg aaaaagcaat tgctgagtag cgttttcaac ggtctcagca
     1921 atttggatac taaacgcgat gtcttacaac gactatcgtt tagagaactg gaatctcttc
     1981 cttgcttttg gacgacctgg ttttttacgt tcaaccatac gtgggtcgcg acgcatcaga
     2041 ccttgttctt ttaaggccgg acgatattct tcgttcagtt ctaccagtgc gcgtgagatg
     2101 gcgagacgca gcgcctcggc ctgtccttta tatccaccgc catcaagcaa tgcttttaca
     2161 tcatattttc cggtagtgcc ggtgatgatg aatggctggt caataatata ttgaagcgtt
     2221 tcgagcggga aataattttt ataatccctg ccattgacag tcattgttcc atttccgggt
     2281 gtcagtctca gacgggcaac ggctgttttt cttcttccta aggtcagtgt ttcagacatg
     2341 tcctttattt gatttcgtta atattcacag atttgggctg ctggccttcg tgtttgtgtt
     2401 cggtaccagc atatacatac aggtttctga acaactcagc gcctaatttt gtttttggaa
     2461 gcatcccttt tacagccatt tctacaacgg cttcaggttt gcgtttcagc aggtcggcag
     2521 gattggtgaa acgctgtccg cccggatatc ctgtgtggcg aacatattcc ttatctgtca
     2581 gcttgttgcc ggtcagtttc actttttctg cattgatgac aatcacattg tccccacagt
     2641 ccgaattcgg tgtgaaactt ggtttgtgtt ttcctctgag catgaatgcc accatggcgg
     2701 caaaacgtcc caaagattgg tctgttgcat caacaataac ccactcttta ttagcgttgg
     2761 ccttgttgat tgatttcgtt ttataactga gcgtgtccat tacaattgtt ttagagagaa
     2821 ataagcccat ttttcaaatc gggctgcaaa ggtagaaata tttttttatt aaccaatacc
     2881 gtttgataaa aaaagtttga aaatagttga tttttactgt tttaatattt tttta
//
LOCUS       DAOPKE010000174         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_38686_length_2914_cov_12.297657, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000174 DAOPKE010000000
VERSION     DAOPKE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38686_length_2914_cov_12.297657"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKY63_12665"
     CDS             <1..364
                     /locus_tag="PKY63_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPF01511.1"
                     /translation="KNILQLSEVSLLRSTFFNIFAFRMDNYHNQYTLGEVLQQLIDKF
                     RLRNRMNSEALQAAWPEIAGALVARHTKSVQLDGPVLYIEVDEPALRNELLYMQSDII
                     KAVNERLHNDAVEKLVIR"
     gene            376..822
                     /locus_tag="PKY63_12670"
     CDS             376..822
                     /locus_tag="PKY63_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF01512.1"
                     /translation="MNIQLRPATEADFPEILGMIKELALFEKAPEKVTNSVEQMQHEK
                     DFFHCIMAENEDGTIVGMALYFFAYYTWVGKSLYLDDLYIKPQYRGNGIGTMLLKEIM
                     NISKEEKCTRIRWQVLDWNTPAIDLYKKMGMTLDGEWINCDWINEK"
     gene            complement(937..2145)
                     /locus_tag="PKY63_12675"
     CDS             complement(937..2145)
                     /locus_tag="PKY63_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01513.1"
                     /translation="MPRYILRPDYIEKVRPYISKPLIKILTGQRRVGKSFIMLQIADV
                     IRKEIKKANIIFVNCETEGFLHLKTYKDLLAFAQGRLIKGQKNFLFIDEVQEIEQFQL
                     ALRSLLAENKCDIYCTGSNAHMLSGELATHLAGRYIEISVHALSFSEFLRFHKLKPEY
                     GSVMKYLTYGGMPYLTNVELTGDLPFEYLRSVYATIMLKDVVARENIRNITFLENLIR
                     YLADNVGSLFSAANISKFLKSQHIAISPQLTLNYLRALTNAYIIHKVQRAEVNGLKIF
                     EIGEKYYFEDTGLCNAITGHHSRNDLHKTLENAVFLHLIQSGYAVQVGKLDQQEIDFV
                     ANRNGLQIYVQVCLQLTDKEVVNREFGNLMQIPDNYPKFVVTLNDSIIGNDYKGIKQI
                     NLLDFLQMNL"
BASE COUNT          925 a          563 c          601 g          825 t
ORIGIN      
        1 gaaaaatatt ttacaattga gcgaagtatc cctacttcgc tcaacatttt ttaatatctt
       61 cgcattccgc atggacaact accacaatca atacacactc ggtgaggttt tgcaacagct
      121 gattgataaa ttcaggctgc gcaaccgcat gaacagcgaa gcgctgcaag cggcctggcc
      181 cgaaattgcc ggagcactgg tggcacgtca caccaaatcg gtgcagctcg atgggcctgt
      241 gttgtatatc gaagtcgatg agcccgcgct gcgcaacgaa ttgctgtaca tgcaaagcga
      301 tatcattaaa gccgtgaatg aacgactcca taacgatgcg gtagaaaaat tagtcatcag
      361 ataatttcaa caagaatgaa tatacagctg cgtccggcca ctgaagccga ctttccggaa
      421 atactcggca tgataaaaga gcttgctttg tttgaaaaag caccggaaaa agtgaccaac
      481 tccgtcgaac agatgcaaca cgagaaagat tttttccact gtatcatggc cgaaaacgaa
      541 gacggaacca tcgtcggcat ggcgctctat ttctttgctt actacacatg ggtagggaag
      601 tcgctctatc tggatgactt gtatatcaag ccgcagtatc gcggaaatgg cattggcact
      661 atgcttttga aagagatcat gaatatttcg aaagaagaaa aatgcacccg tatccgctgg
      721 caggtcctcg actggaacac accggccatt gacctttaca aaaaaatggg catgaccctc
      781 gacggcgagt ggattaattg tgattggatc aatgagaagt agccagttgt gtcccgatag
      841 caattgggaa gtcagtagcc agtctgccgc ggctaagtga gtaattttta atgataaagg
      901 aatacagaat gcaagaatag aatcttgctg acacaattac aaattcattt gaagaaaatc
      961 cagcagattt atttgtttta ttcctttgta atcatttccg attatagagt cgttcagagt
     1021 aacaacaaat ttcggataat tgtcaggtat ttgcatcaga ttaccgaatt cgcgattgac
     1081 aacttcttta tcggtaagtt gcagacacac ctgaacataa atctgaagtc cgttacggtt
     1141 ggcaacgaaa tcgatttcct gttgatcaag ttttccaacc tgcacagcat atcccgattg
     1201 aatcaagtgc aaaaacacag cgttttcaag tgttttatgt aaatcgtttc tggagtgatg
     1261 gccagtaata gcattacata atccggtatc ttcgaaataa tacttttcac ctatttcaaa
     1321 tattttcaat ccatttactt cggctcgttg aaccttatga ataatgtaag catttgtcag
     1381 agcccgaaga taattcagtg tcagttgcgg agatatagca atgtgctgcg acttcagaaa
     1441 tttactaatg tttgcagcag aaaaaaggct tcctacattg tcggccagat aacggatcag
     1501 gttttccaga aatgttatat tccgtatgtt ttcacgggca accacatctt tcaacataat
     1561 ggttgcatat acactgcgaa gatattcaaa cggcaggtct ccagtcagct caacatttgt
     1621 aagataaggc attccaccat aggtgaggta tttcatcaca ctgccatact caggttttaa
     1681 tttatgaaaa cgaagaaact cactaaaact gagcgcatga acagaaatct caatatatct
     1741 tccagctaaa tgggtggcca gttctcccga taacatgtgc gcattgcttc cggtacaata
     1801 aatatcgcat ttgttttcag caagcaaact ccgcaaggcc agctgaaatt gctcgatctc
     1861 ctgtacttca tcaatgaaaa gaaaattctt ttgtcctttt atcaggcgtc cctgtgcaaa
     1921 tgccaacaaa tctttataag tctttaaatg aaggaaccct tcagtctcgc aattgacaaa
     1981 aataatattg gcctttttaa tttctttgcg gataacatcg gctatttgca gcataataaa
     2041 gcttttgccg actcttcgtt gccccgtaag aatctttatc agaggctttg agatatacgg
     2101 acggactttt tcaatataat cgggacgtag aatgtatctg ggcataatag tttcatttat
     2161 acatgaatga ttttacaaat atacgaaata tttcatgtat aacgtaaata taattcacaa
     2221 attaatattc cggaaactac aaagttgggt atgaccctcg atggcgagtg gattaattat
     2281 gactggatca atgaatagca gtgagcggtg agtaatgagc agtgaactta gttgggtgtg
     2341 gtcagtggtg agtggttaat ggttaacggt taatggtagt gtcagttgtg agtagccagt
     2401 agccagtttt atgatgttgt tacaaacgtt tatcccaaaa attatcattg tcgattcctt
     2461 tgaaaacgac tacggatgta gtcgaataat aatagcccga tgccctgatt gctgagttca
     2521 cgaagttcag ggctacggga acaagaagag cgaagcacct ttgaaaaacg agatgtagaa
     2581 tagaaaccca aaaaaagtat cccccctgtt taatgatttg tagaaaaaat catataaatt
     2641 tgcatattag caagaccaac aactatcaga cctggtctat gtgatttggt ggaattggtc
     2701 ggatgatgtg aggcctataa acaagttgtc agtaacctaa tagtaagtga tgtacacatc
     2761 tgaacaaata gaattattcg acaaggtctt caattattat gttgactttg aaattacaaa
     2821 aaaggaaagg aacaatccta ctgaaacttt gttttctaaa aagtatccat gcaataaaaa
     2881 atggtctttg agtaagttgt taagagtaga ctaa
//
LOCUS       DAOPKE010000175         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_39583_length_2867_cov_14.232930, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000175 DAOPKE010000000
VERSION     DAOPKE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39583_length_2867_cov_14.232930"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKY63_12680"
     CDS             <1..371
                     /locus_tag="PKY63_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01514.1"
                     /translation="AAGQNELSQNNNPVGTTGNTNPINVQINQQEQVNYENNIYNTNN
                     DVQQKSEGGIQIQAGSRSRGTSSPSVSYSSSGSGKAKHSFVKDISKSIKAFQYKHSGK
                     KKFRRHGGKHSRSSILRCFN"
     gene            677..1966
                     /locus_tag="PKY63_12685"
     CDS             677..1966
                     /locus_tag="PKY63_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF01515.1"
                     /translation="MSVFKTLRSFPRNFYTVIVMEFFERGSYYGVMSVLSVYLALGVN
                     EGGLGFSKESIGVIKSTVTPLLYFLPILSGALAARFGYKKILSLAFVLMSSGYALSST
                     VTSYPMIFLCLLLMAVGAGLFKPLIAGTIAKNTDESNSSLGFGMYYWSINLGAFIFPL
                     ILIPWMKSFGWSYIFIMAAVGTGWLFFLNLLTFREDKKKEKQKTIGETMLEMVMVVAN
                     VRFILLIIVYSLFWVMYFQMYDSVLWYVTEEVDMSAVNTAMNGFLSGFIENPQWKFDA
                     EHVTVVNAGVIILLQLFVSAILKKTKPLPTMMVGILFGSIGMAVLAFSTEGWIFLLGC
                     VIFTLGEMTAHPKFISYVGLIAPADKKALYQGYAFLYGVIGSGVGGILGANLYVYYVE
                     QNNNPSALWLTFSGIGILSVVLLFAYNRLFGRAKKTQ"
BASE COUNT          773 a          652 c          651 g          791 t
ORIGIN      
        1 tggcagcggg ccagaacgag ctgagccaga acaacaatcc ggtgggcaca accggtaata
       61 cgaatcccat aaatgttcag atcaatcaac aggagcaggt taattacgaa aataacatct
      121 acaataccaa caacgacgtt cagcaaaaga gcgaaggcgg aattcagatt caggccggat
      181 cgcgcagcag agggacctct tcgccatcgg tgtcgtattc ttcatcagga tcaggaaagg
      241 caaaacacag ttttgtaaaa gacatttcta agagcatcaa agcgtttcaa tacaaacaca
      301 gcggaaaaaa gaaattccgc cgccacggtg gcaaacacag ccgcagcagt attctcagat
      361 gttttaatta gcatagttga ggatgtctca aaaacaaact aagcacaacg acattccata
      421 gttcagcaag ctttgccggc aatttagctt ctggcgagga acatggaata gtcgcataag
      481 atacagtctt gtgcacgaca caaaaacaac gctttaggca ctaacgactc ttccatgtct
      541 ttcatttcat tccagactat ggaaggtcgg ttcgcttgag gaagttgttg tttttgttgt
      601 tacattccat tttgagacag tctcaacttg aattttttgg tcagctgtgc gaagatttct
      661 atcttcgcac ccacctatgt cagtattcaa aacacttcgc tcctttccgc gcaatttcta
      721 caccgtcatt gtgatggaat ttttcgaacg cggctcttat tacggcgtca tgtcggtgtt
      781 gtcggtatat ctcgcacttg gtgtaaacga aggcgggctg ggcttttcga aagaaagcat
      841 cggcgtaatt aaaagcacgg tcactcccct gctctatttc ctgcccattc tttcgggcgc
      901 gctggcagct cgtttcggat ataaaaaaat tctctcactg gcgtttgtac tcatgagttc
      961 gggctacgca ttatcgagta cggtcacatc gtatccgatg atttttttat gtctgctact
     1021 gatggctgtc ggagccggat tattcaaacc actgattgca ggaacgattg cgaagaacac
     1081 cgatgaaagc aattcgtcgc tgggctttgg aatgtattac tggtccatca atctgggcgc
     1141 attcatcttt ccgctgattc tcattccatg gatgaaatca tttggatgga gctacatttt
     1201 catcatggcg gccgttggca ccggatggtt atttttcctg aatctgctta cgttccgtga
     1261 agacaaaaaa aaggaaaagc aaaaaaccat tggcgagaca atgctcgaaa tggtcatggt
     1321 agttgccaat gtccgcttca ttttactcat catcgtttac tcgctgttct gggtcatgta
     1381 ttttcagatg tacgactctg ttctgtggta tgtaaccgaa gaagtggaca tgagtgccgt
     1441 aaacacagcg atgaacggat tcctatccgg ctttattgaa aacccgcagt ggaagttcga
     1501 tgcagagcat gtgacggtag tgaatgccgg tgtgattatc ttgctgcaat tgtttgtctc
     1561 agctatatta aaaaaaacaa aaccgctacc caccatgatg gtcggcattt tattcgggtc
     1621 gatcggaatg gcagtactgg ccttttctac cgaaggatgg atatttttat tgggttgtgt
     1681 gattttcaca ttgggtgaaa tgactgcgca tccgaaattt atttcatatg tcggactaat
     1741 tgcgccagct gataaaaagg cattgtatca gggctatgca tttctgtacg gcgtgattgg
     1801 aagcggtgtt ggaggcatac tgggtgcaaa tctttatgtc tattatgtag agcagaacaa
     1861 caatccatcg gcgctgtggc tcacattcag tggcatcgga atattgtctg ttgttttatt
     1921 gtttgcatac aaccgcctgt ttgggcgcgc aaagaaaacg caatagtttc gcgcaacgaa
     1981 atttgccacg aggacaccac gacaccatga tttacgattc ccgattgaag cctttcgtct
     2041 tcgctcagga taaaaatttc tgattcttga tctgaagtca gaaaataatg aagtaccttt
     2101 ctacccgata gttatcgggt ttattaacat gataaacttt taactactga gttcccggag
     2161 agaatcttac aacggcgcct ttatgacttg ttaactttca actttacaaa ctcagcggtg
     2221 ccgggatctc ctggaaaacc ttcgcagcat aagcctcttt tgtccgcaga ttaagcagat
     2281 gcacgcagat acctcagcag atctaattga cgcagattat aatcatcctt catctgcgat
     2341 gaagactgat tcagaggagc gttcttatcc acgacattta agccccgcca aatgcggggt
     2401 aaactccagc aagaaaaggt tgacgccaca gcttactgac ctggaatttc atctgactgg
     2461 caaggcaaac gtcggggacc tgtcctatag caccaaacga ttccgtgtcc gctccctttc
     2521 ttgaaaaaag tccggcggac gccaaaatgt tggctgcgct tcccgacatt ttagcgtcag
     2581 ccagctatgg ttgacattag acctgctgac ctggaatcgt cgtggagaaa agctttagca
     2641 cccaacagcc tgtccgccct ctggcggaat tccgggtccg ctcccattcg caaatcgctg
     2701 tctgtcggac gccaaaatgt tgggtgccgt ctatgaagac ggattcatta gtgtcgctga
     2761 gaaagcagat tagccatgcg ctatgcttta tgccttttac atcgccttta tgaacttgat
     2821 aactcctatg aataaaaccg cctaaaagca agattaagag cagttta
//
LOCUS       DAOPKE010000176         2703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_43043_length_2703_cov_14.694864, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000176 DAOPKE010000000
VERSION     DAOPKE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43043_length_2703_cov_14.694864"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PKY63_12690"
     CDS             <1..407
                     /locus_tag="PKY63_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPF01516.1"
                     /translation="CRTKLRYGAPQTFSAGPKYLATVLRNKKCLVYAINGTADHVHIL
                     FTLHPSVSLSDLIKDLKLSTSSFIRTKKLFPMFDGWQEGYGAFTYTYSAKENLIRYVS
                     NQQEHHKKISFYEELKNLLIENEIDFDEGYLI"
     gene            667..1137
                     /locus_tag="PKY63_12695"
     CDS             667..1137
                     /locus_tag="PKY63_12695"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HPF01517.1"
                     /translation="MRKAFYPLLMILVLTGCNVAKHPSKAIKKLAFLQGNWQYEDPGV
                     QMIEKWETAPDAMHGTSLLILKGDTVFQENIVLKDIDGKIYYKSTMGKYILEDLKTLA
                     LTRCSSRRAVFGSMNQINSVYICYSYKRGKLFVEMRDIVDGKVIHDKYGLVKIN"
     gene            1137..1793
                     /locus_tag="PKY63_12700"
     CDS             1137..1793
                     /locus_tag="PKY63_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF01518.1"
                     /translation="MSIEIKEICKSFGSLQVLKGINLSIQKNEIISILGASGAGKTTL
                     LHIMGTLDKPDSGQILYDGTDVFSLGDRKLNNFRNKHIGFVFQFHHLLPEFTALENVC
                     IPAFIGRMDKAEATAKAESLLVTLGLKDRMTHKPSALSGGEQQRVAVARALINDPDVI
                     LADEPSGNLDTDHARELHELFLKLRNEMNQTFVIITHNPDLAQLSDRQIHMRDGYLEQ
                     "
     gene            1774..2646
                     /locus_tag="PKY63_12705"
     CDS             1774..2646
                     /locus_tag="PKY63_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPF01519.1"
                     /translation="MVISNNKKIFIAGGRGFIGQTLCRELESSGFSVVILSRSEYQSN
                     PTKLAEKIDGSFALINLAGATINKRWTENYKTEILESRIQSTTTIAQAIDLCKQPPER
                     FISTSAVGIYDSFHIHTEQSTSFADGFLSEVCMKWEASTTIFDRTTKRTIIRLGVVLG
                     MNGGMLKKLKPLFKLYLGGRMGSGNQLFPFIHIQDVAGAIIHILSMQSPAEIYNLVSP
                     KIITNKQFTRAFAKALNRPAIWTVPAFALKIILGNRSALILDGQKVIPEKLQQECYVF
                     RFPDVKDAEFWENT"
BASE COUNT          854 a          559 c          554 g          736 t
ORIGIN      
        1 tgtgccgtac gaagttacgg tatggcgcac ctcagacttt ttcagcaggc cctaaatatc
       61 tcgctacggt attgcgaaat aaaaaatgtt tagtgtatgc aatcaacggt acagcagatc
      121 atgtgcacat attatttacg ttgcatccat cggtctcttt atcggacttg ataaaggatc
      181 taaaattgtc aacatcgtca tttatccgca caaaaaaatt gttccccatg tttgatggat
      241 ggcaggaagg gtatggtgca tttacttata catattctgc aaaagaaaat ctaatcagat
      301 atgtttcaaa tcaacaagaa caccataaga aaattagctt ttatgaggag ctgaaaaatc
      361 tcctgattga aaatgaaatt gattttgatg aggggtattt gatttaatct gacttcattt
      421 aatctggatt cgaccccttc agggtcggct ttgtcgtttg catataaccc gacctacggt
      481 cgggctatta ttatttgacc tgcgctgcag gtcgggtttt ttacatgcaa ttaattaatc
      541 tattgctttt gtctataaca aatttgaaaa acaaaatcag gtgccctggt agcgaagcca
      601 ggtgagggtc actgaacgga atttatccga ttcaatactt ttttgtaagt ttgaaaaaaa
      661 attgcgatga gaaaagcgtt ttatccgctt ctgatgattc ttgtacttac tggctgcaac
      721 gtagccaaac atccttcaaa agccataaaa aaactggcgt ttttacaagg gaactggcaa
      781 tacgaagacc cgggagtaca aatgattgaa aaatgggaaa cagctcctga tgccatgcat
      841 ggaaccagtc ttctgattct gaaaggcgac actgtgtttc aggaaaacat tgtattgaaa
      901 gatattgacg gaaagatata ttacaaatcc accatgggga aatatattct tgaagatctg
      961 aaaactttgg cgctcacccg gtgctcttcg cgcagagccg tatttggcag tatgaatcag
     1021 atcaactccg tgtacatatg ctacagttat aaacgtggaa aactgtttgt tgaaatgcgc
     1081 gacattgttg atggcaaagt aattcacgac aaatacggac tcgtaaaaat caactgatgt
     1141 caattgaaat caaagaaata tgcaaatcat tcggatccct gcaagtgctg aaaggcatta
     1201 atctgagcat tcagaaaaac gaaatcattt caattctggg tgcatcggga gccggaaaaa
     1261 caacgctgct gcatattatg gggacactgg ataagcccga cagcggtcag attttatatg
     1321 acggaaccga cgtgttctcg cttggagatc gaaaactcaa taatttcaga aacaaacaca
     1381 tcggattcgt ttttcagttt catcatttac taccggaatt cacagcgctg gaaaacgttt
     1441 gcatccctgc attcataggc agaatggaca aagccgaagc aacggcaaaa gccgaaagtc
     1501 ttctcgttac actcggactc aaagaccgaa tgacacataa accatctgca ttatctggcg
     1561 gagaacaaca aagagttgct gttgcgcgtg cgcttatcaa tgatcccgac gtcattctgg
     1621 ccgacgaacc cagtggcaat cttgacaccg atcacgcacg tgaattacat gaattgtttc
     1681 tgaaactgcg caacgaaatg aatcagactt ttgtgattat cactcacaac ccagatttgg
     1741 cgcagctttc cgaccgacag atacatatgc gtgatggtta tctcgaacaa taaaaaaata
     1801 tttattgcgg gtggtcgcgg atttatcggc cagacactgt gcagagaact ggaatcatcc
     1861 ggattttcag ttgttattct ttcacgttca gaatatcaga gcaatccaac aaaacttgca
     1921 gaaaaaattg atgggagttt tgccctgatc aatctggctg gtgccaccat caataaacgc
     1981 tggaccgaaa actacaaaac agaaattctt gaaagccgca ttcaatcaac cacaactatt
     2041 gctcaggcaa tagacttgtg taaacagcca ccagagcgat ttatcagcac atcggctgtt
     2101 ggcatctatg actcttttca tattcatacc gaacaaagca catcctttgc cgatggcttc
     2161 ctgtcagaag tttgcatgaa atgggaagca tcaacgacaa tttttgatag aactacaaaa
     2221 cgcaccatca taagattggg agtcgtgctt ggaatgaacg gaggaatgct gaaaaagctg
     2281 aaaccattat tcaaactata tcttggagga cgcatgggaa gcggaaatca gttattccca
     2341 ttcattcaca ttcaagatgt ggccggcgca atcatccata ttctctcaat gcaatcgcct
     2401 gccgaaatat acaatctggt ttcacccaaa atcattacca ataagcaatt cacaagagcg
     2461 tttgctaaag cattaaaccg gccagcaata tggactgttc cagcatttgc gctaaaaata
     2521 atattaggaa acagatcagc acttatcctt gacgggcaga aagtaattcc tgaaaagctt
     2581 caacaagagt gctatgtatt cagatttcca gatgtaaaag atgctgaatt ctgggagaac
     2641 acttagtagc ggcgtctttt accagcactt ggttttttat cttttttcca cgacttgtcg
     2701 tcg
//
LOCUS       DAOPKE010000177         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_48726_length_2480_cov_9.314639, whole genome shotgun sequence.
ACCESSION   DAOPKE010000177 DAOPKE010000000
VERSION     DAOPKE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48726_length_2480_cov_9.314639"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2258
                     /locus_tag="PKY63_12710"
     CDS             <1..2258
                     /locus_tag="PKY63_12710"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01520.1"
                     /translation="STCSGFFYDNGGMSGNYSDNQNDTITFCSAGGGYITVDFTTCDL
                     VAGDHLYVYYGDVASGTPNTTNPTTGLIVSTCPCITLVFISDAAGNKSGWAATIGCTT
                     PSTIANDDIVYSTEPTLDGTLLSGQTNLGADADIFSGCFDAGNTVWYGFSLSGGNNTI
                     STTISDASFTDVQLLLLHSYNCSDGSAVIIGSECTTTGNTVTWTNLTAAYYWLGIASP
                     DAGDFSISFTESYQDFCGDYFCGPGESCLTCPFDCGICPEAEGGPYYHPITGMQNTKL
                     GQCLVSTNVGNYYDNGGPGMTYASDINNIFRTFCPSSPHYTIEATVENMSLEYAGVNC
                     KDQLLITNGPSPGSALLWSGCGNSSTSAIRTTDGVYNGGVFKSTHSSGCLTFSLITDA
                     SNAGFWDGWTITLKEVTYASGPELNYNNDCERAIPLCDDFTVSSQMYGPGLSAESCAS
                     CILTESFSEWYKVKVSHGGTIEFEITPLGNSDMDFAIYRADSCGALTTPVRCSHALYQ
                     PPGKTGLSKASGDMSEDVNGDQWVSELAIETGATYYILINECNKLNPNAYTLDFKLSD
                     GASFDCSIVLPVEFLDFNASVKTASIELTWKTASETNNDYFTIERSLDGENFIPLAFI
                     DGNGNSNEIVRYFYTDESPFQGLAYYRIKQTDFDGKSDYSQVLPIDFMDQKLPWLNVY
                     PSPATDFIHVSTDETSINQPYNICNALGAIVETGICQKTEFNISIADFEEGAYYFSIN
                     NQQQMYFIVSQ"
BASE COUNT          699 a          628 c          520 g          633 t
ORIGIN      
        1 taagcacatg cagcggcttt ttttatgata acggaggcat gtccgggaat tactccgaca
       61 atcagaatga caccataacg ttctgttcgg caggcggcgg ctatatcacg gttgatttca
      121 caacctgcga tctggtggcc ggagaccatt tatatgtgta ttacggcgat gtggcttcgg
      181 gcacaccaaa cacaacaaat cccacaaccg gactcattgt ttcaacctgt ccctgcataa
      241 cgctggtatt tatttcagat gcagccggca ataaatccgg atgggccgcc accattggat
      301 gtaccacacc ttcaactatt gccaacgacg atattgttta ttccacagaa ccaacgcttg
      361 acggcacttt attatcggga caaaccaacc ttggagccga tgcagatatc ttttccggat
      421 gtttcgatgc cggaaatacc gtgtggtacg gattttcact ttcaggaggc aataatacca
      481 tcagcactac aatttccgat gcaagtttca ccgatgtaca attgttgctt ctgcacagct
      541 acaactgctc cgatggctct gccgttataa tcggaagtga atgcacaaca accggcaata
      601 ctgtaacctg gaccaatctt acagccgcat actactggct tggaatagca tcgcctgatg
      661 caggggactt cagcatcagc tttaccgaaa gttatcagga tttctgcggc gactatttct
      721 gtggcccagg cgaatcgtgc ctgacctgtc cgttcgactg cggaatctgt cctgaagccg
      781 aaggcggacc ttattaccac cccatcaccg gaatgcagaa taccaaactg gggcaatgtc
      841 tggtttcgac caatgtcgga aactactacg ataacggtgg acctggaatg acctatgctt
      901 cagatataaa caatattttt cgcacctttt gcccatcatc tccacactac accattgaag
      961 ccactgtaga aaacatgagt cttgaatacg ccggagtgaa ttgcaaagat caattactga
     1021 taaccaacgg gccttcgccc ggcagcgccc tactctggag cggttgcgga aacagctcaa
     1081 catcggccat acgtactacc gatggtgttt acaacggagg tgtttttaaa tcgactcatt
     1141 ccagcggatg tctgacattt tcacttatta ccgacgcttc caatgctggt ttctgggatg
     1201 gatggacaat aaccctgaaa gaagtgactt atgcatcagg accagaacta aactacaaca
     1261 atgattgcga aagagccatt ccgctgtgcg acgattttac cgtgtcttcg cagatgtacg
     1321 gacccggcct ttctgccgaa tcctgtgcaa gctgcattct gacagaaagc ttctcggaat
     1381 ggtacaaagt gaaagtcagc catggtggca ccattgaatt tgaaatcaca ccacttggca
     1441 acagcgacat ggattttgca atctaccgcg cagattcatg cggcgccctg accacccctg
     1501 ttcgatgctc gcatgcactg tatcagccgc cgggaaaaac aggattatca aaagcatccg
     1561 gcgacatgtc agaagatgta aacggtgatc agtgggtatc ggaacttgcc attgaaaccg
     1621 gtgccaccta ctacatcctc atcaacgaat gcaacaagtt gaatccgaat gcctatactc
     1681 tcgacttcaa actttcggat ggtgcttcgt ttgactgttc cattgtactt cccgttgagt
     1741 ttctcgattt caatgcttcg gttaaaaccg catcgattga attgacctgg aaaaccgcct
     1801 cagaaaccaa caacgactac tttaccattg aacggtcgct ggacggagaa aatttcatac
     1861 cactggcatt tattgacgga aacggcaaca gcaatgagat agtgcgctat ttctacaccg
     1921 acgaaagccc atttcagggt ctggcctatt acagaatcaa acaaaccgat tttgacggaa
     1981 agagtgatta cagccaggtt ttgccgattg actttatgga tcaaaaactt ccttggctca
     2041 atgtatatcc aagtcctgct accgatttta ttcatgtctc taccgacgag acatccataa
     2101 accaaccata caacatctgc aacgccctag gagccattgt tgaaaccggc atttgccaaa
     2161 aaacagaatt caatatttca atagccgatt ttgaagaagg tgcctattat ttttcaataa
     2221 acaaccaaca acaaatgtac ttcattgtat cacaataaac ctcctcgtaa atgtaataga
     2281 aaaccccgtc agcgcttgtt gttacggggt ttttgttatt gcagaaaacc tgttcaaacc
     2341 ggtcccgaaa atcaaaaaaa ctaatgaaat ttcaccccat aaaaaaaggc ctcaaacaca
     2401 tggtctgaag ccgattttcg tttttgtgag tcttggcagg ttcgaactgc cgacctcttg
     2461 cctgtcaagc aagcgctcta
//
LOCUS       DAOPKE010000178         2469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_49032_length_2469_cov_8.904308, whole genome shotgun sequence.
ACCESSION   DAOPKE010000178 DAOPKE010000000
VERSION     DAOPKE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49032_length_2469_cov_8.904308"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKY63_12715"
     CDS             complement(<1..218)
                     /locus_tag="PKY63_12715"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPF01521.1"
                     /translation="MKAILFSIMMMAAFGLSRAQIVYVTEWKSEADKVVYVSDWKSEA
                     DMIVYVSDWKSEAMPDSGIWYFTEWKSEA"
     gene            complement(317..802)
                     /locus_tag="PKY63_12720"
     CDS             complement(317..802)
                     /locus_tag="PKY63_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF01522.1"
                     /translation="MYEVRKKEGGKVLFGGTKVEGKKNLSGHYVVPALVEAENHYKIV
                     QEETFAPILYLIKYDGDIENAIEINNAVPQGLSSSLFTLNMREAEKFLSDAGSDCGIA
                     NVNAGTSGAEIGGAFGGEKDTGVGRESGSDSWKQYMRRQTNMINYSNELPLAQGIKFE
                     F"
     gene            complement(803..2302)
                     /locus_tag="PKY63_12725"
     CDS             complement(803..2302)
                     /locus_tag="PKY63_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPF01523.1"
                     /translation="MLKIRKVRTKSGSIAFQVVQYVGHRVKIEKHIGSAQNEEQCKAL
                     HSLANEWIEKHIAQTTLFPAERQKTLFVENAKCIAVTHHFAYHFFAKCVDACGLSQLP
                     QLLIDLAIMRLIEPSSKLQAISLLARYFGKQYSQRIYQTIPKFIEHKTAIEKQAFKTV
                     RDTFEEQFYYVLYDVTTLYFESFKADELRIQGFSKDNKSQQPQIVIGLLVTQSGFPLS
                     YNVFPGNTFEGKTMLPVIQNFIDEHPNTKPIVVADAAMLDEDRLAELRNKKISYIVGA
                     RLANANIKLVKHIYTALNTDNGATARMQTQHGDLICDFSTKRYKKELNELNKLIQKAE
                     DLVAKQSLGNKAKFVKRVSKEKIELNTALIEKRKLLLGIKGYCTDLSEDQLSNNDVID
                     RYHQLWHVEQSFRMSKFDLQARPIYHRNEDAVRSHVLICFVALMAEKYLELKTNTSLR
                     KIRILVWDLTESHIQDSITKEVFKFSSPTSEIMNTSLAPLINEWNLLPH"
BASE COUNT          698 a          518 c          441 g          812 t
ORIGIN      
        1 gcttcgcttt tccattcagt aaaataccag atgccggaat cgggcatggc ctcactcttc
       61 caatcactga cataaacgat catgtcggcc tcacttttcc aatcgctcac atagactact
      121 ttatcggctt cgcttttcca ctccgtaaca tacacaatct gcgcgcgcga caggccaaat
      181 gcagccatca tcattattga gaataatatt gctttcatga ttcagtattt ttgaagctga
      241 ttacaccaat tattataccg catgaaaaaa aagcacgacc tgtttgatcg tgcttaacag
      301 agttttggaa cttatatcag aattcgaatt taattccctg agccagcgga agttcgttgc
      361 tgtaattaat catattcgtc tgacggcgca tgtattgttt ccagctgtca gagccagatt
      421 cacgacccac gccggtatct ttttcgccac caaaagcccc accgatttca gcaccggatg
      481 taccagcatt gacattggca attccgcaat cggaaccggc atctgagaga aatttttcag
      541 cttcgcgcat gttcaatgta aaaagcgaag acgacaatcc ctgtggtaca gcgttgttaa
      601 tttcaatagc gttttcgata tcaccatcat attttatcag atataaaatc ggtgcaaaag
      661 tttcttcctg cacaatttta taatgattct cagcttcaac caaggccgga acgacatagt
      721 ggccactcag atttttttta ccttcaacct tggttccgcc aaacagaacc tttcctcctt
      781 cttttttcct gacttcatac aattagtgcg gtagaagatt ccattcatta atcaaaggtg
      841 ctaaggaggt attcataatt tccgaagtcg gagatgaaaa tttgaaaact tccttggtta
      901 tggagtcttg aatatgtgat tcagtcaggt cccacacaag tattctgatt tttcttagag
      961 atgtatttgt ttttaactca aggtattttt cagccatcaa tgcaacaaaa caaataagta
     1021 catgagatcg cacggcatct tcgttgcgat gataaattgg tcgagcctgc agatcgaatt
     1081 tgctcattct gaaagattgc tctacatgcc acaactgatg atatctgtca attacatcat
     1141 tgtttgacaa ctgatcttca gacaaatctg tgcaatagcc ttttattccc aacaataatt
     1201 ttcttttctc aatcaatgct gtgttgagct ctattttttc ttttgatacg cgttttacaa
     1261 actttgcttt attgcccaaa gactgtttag ctacaagatc ttctgctttt tgaattagct
     1321 tgtttaattc attcaattct tttttatatc gtttcgttga gaagtcgcaa atgaggtcgc
     1381 cgtgttgtgt ttgcatacgt gctgttgccc cattgtcagt atttaaagct gtgtaaatat
     1441 gttttaccaa cttgatattt gcgtttgcca atcgggctcc gacaatgtat gaaatcttct
     1501 tgttgcgtag ttctgctaat ctatcttcat ccagcattgc cgcatctgca acaacaatag
     1561 gctttgtatt tgggtgctca tcaataaaat tttgaatgac aggaagcatt gttttacctt
     1621 caaatgtatt gcctggaaac acattgtatg atagcggaaa accggattga gtaacaagca
     1681 atccaattac tatctgtggt tgctgagatt tattgtcttt gctgaacccc tgtatcctta
     1741 attcatcagc tttaaatgat tcaaaataca aagtagtgac atcgtaaagc acatagtaaa
     1801 attgctcttc aaatgtatcc ctgacggtct taaatgcttg cttttcaatg gcagttttgt
     1861 gctcaataaa tttgggaata gtttgataaa tgcgctgaga atattgtttc ccaaaatagc
     1921 gcgccagcaa acttattgct tgaagtttcg atgatggctc tattaatctc ataatcgcca
     1981 aatcaatcaa caattgaggc agttgagaca acccgcaagc gtctacacat tttgcgaaga
     2041 aatgatacgc aaaatggtgt gtgacagcaa tgcatttggc attttcaaca aatagcgttt
     2101 tttgtctttc agcaggaaac aaagttgttt gagcaatgtg tttttcaatc cattcgttcg
     2161 ctaaagaatg caaggcttta cactgttctt cattttgagc gctgccaatg tgtttttcaa
     2221 ttttaacccg atgtcccacg tattgaacaa cctgaaatgc aatagatcca gattttgttc
     2281 ttactttcct aattttgagc atagtaaaaa agcgttagtg cggtaaagca acaaaaatac
     2341 gatctttatt caacttatta tcaacaagtt aaaattttac caccgcacta atttcgatag
     2401 gcgtatgaag tcagggccgg aacgacatag tggccactca gatttttttt accttcaacc
     2461 ttggttccg
//
LOCUS       DAOPKE010000179         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_49700_length_2446_cov_12.494772, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000179 DAOPKE010000000
VERSION     DAOPKE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49700_length_2446_cov_12.494772"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>2446)
                     /locus_tag="PKY63_12730"
     CDS             complement(93..>2446)
                     /locus_tag="PKY63_12730"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPF01524.1"
                     /translation="AYICIASLRKKQVFRGAQKVREKRRLYLGSLDKYKCRIDRKLSL
                     MRDEPRQMYDSIRQANADSLNEASGSDMFKTPRQRRAERKKARIERRTIRKMRKEHAD
                     TLKAETDSVRIQHIGDLNEFILTEFYDQNMSRQLVDAENTYELADVDDYIYISIGYEE
                     EGMEIDHIQFVYSSPYMIFNSAEDWKFNFYDAQLRKEMLCQQPLVSPLSPMGPTLYKY
                     DYAGLHYDDSAKIFKIKVTPVFPNDALFAGFIYIRDSVFCLDEIDLTINPRALTMVNS
                     FRIHQKYVLMDSVHSVPTETTLEYGIKEGRVNLVTTGTSLFSEYIFDSDSTVFPKHTL
                     EMVRYEDEALERDSLWWTINRRIQLTNQEIIYSDYIDSLRNVFSGEEFTRRLDSAYNH
                     IDIWSFLIKGIRHRNRERQTEFYIGALPTQVNFFGIGGYRHNLNGSFSKRFKNDILLD
                     TDADVNYGFSNRDLRGRVGLGLTYIPKKFVRTYVRFGDYYDMINNNPSITSAFSRSNY
                     ARTKMFAVSQRMEIINGLFGELTFEHSDQQAISDMNMDNWSNQIFGEINEPIDFDRYI
                     KSEIKLNFVYRIRQRYMFKGNRKILLGSKWPDLTFTWRKGIPGLLNSEVNYDYLEFGA
                     GDYVKLNRYGTSNWDFLIGSYVNKKNLRILENKFFRGSDAFFFSNPLVSFQLLGPTLS
                     TNSAFVRGNYIHHFEGIFDKVPLLGRLKLSVAAGGGFLLMDENNFRHGEFFAGLEKPI
                     RIRRELFRLGVYAVTSDNSVTAADFTLKFGISFYNSFRKKWDY"
BASE COUNT          694 a          559 c          491 g          702 t
ORIGIN      
        1 ccggacaaaa atattgttta aacagccctc gaaggaagtt atcagccctc gaagggttcc
       61 aaacccttcg agggcttggc ttagggcttg gctcaataat cccatttctt ccgaaatgaa
      121 ttgtaaaaac ttattccgaa ttttaaagta aaatcagcag ctgtaactga attatccgat
      181 gttacagcat aaacgcccag ccgaaagagt tcgcggcgga tgcggattgg tttttcaagc
      241 ccggcaaaaa attccccgtg ccggaaattg ttctcatcca tcagcagaaa tccgccgcca
      301 gctgcaacag atagtttcaa acgcccaagc agaggtactt tgtcgaaaat tccttcaaaa
      361 tggtgaatgt aattccctct tacaaaagcg gagttggtgc ttaaggtcgg ccccagcaac
      421 tgaaaagaca ctagcggatt gctgaagaaa aaggcatcgc tgcccctgaa aaacttgttt
      481 tcaagtatcc gtagattttt tttattgaca tatgatccta taagaaaatc ccagttggat
      541 gttccatagc ggttcagctt tacataatcc cctgcgccga attcgagata atcataattg
      601 acctcgctgt tcagcaaacc cggaatccct ttgcgccatg taaaagtgag atctggccat
      661 ttactgccca gaagaatttt tcggttaccc ttaaacatat atcgttggcg aatacgataa
      721 acaaaattca gtttgatctc gcttttgata taacggtcaa aatcaatcgg ctcattgatt
      781 tcaccaaaaa tctgattcga ccagttatcc atattcatgt cgctgatggc ctgctggtca
      841 gaatgttcaa aagtgagctc accgaaaagt ccattgataa tttccattcg ctgcgaaaca
      901 gcaaacattt ttgtccgtgc ataattactg cggctgaatg cactggtaat ggacggattg
      961 ttattgatca tgtcatagta atcaccaaag cgaacgtaag tgcggacaaa tttttttggg
     1021 atgtaagtaa gtcccaaccc tactctgcct ctcaaatcac gatttgaaaa tccataattg
     1081 acatcggcat cggtatcaag caatatatca tttttaaaac gtttgctgaa actgccattc
     1141 aggttgtgac gataaccacc aattccgaaa aaattaacct gcgttggcaa agccccaatg
     1201 taaaactcag tctgtcgctc acggtttctg tgacgaatgc ctttaataag aaaactccag
     1261 atgtcaatgt gattataggc agagtcgagt ctgcgggtaa attcttcgcc ggaaaacaca
     1321 tttcgcagac tgtcgatgta gtcggaataa atgatttcct gattggtgag ttgtatgcgt
     1381 ctgtttatgg tccaccacaa cgaatcgcgc tccagggctt catcttcgta acgtaccatt
     1441 tcaagagtat gctttggaaa aacagtggag tccgaatcaa atatatactc cgaaaatagc
     1501 gatgtgcccg tcgtaaccaa atttacccgc ccttctttaa tcccatattc gagcgtcgtt
     1561 tcggtaggta ctgaatgtac cgaatccatc aaaacatact tctggtgaat cctgaatgag
     1621 ttcaccatgg ttaatgcccg tggattaatc gtcagatcta tttcatcaag gcaaaacaca
     1681 ctgtcgcgga tatatatgaa acccgcaaat aatgcatcgt ttggaaatac aggtgttacc
     1741 ttgattttaa atattttggc agaatcatca tagtgcaatc cggcataatc atatttatac
     1801 aacgtcgggc ccataggtga aagcggcgat acaaggggct gctggcacag catttctttt
     1861 cgcagctgcg catcgtaaaa gttaaatttc caatcttcgg cagaattgaa aatcatgtat
     1921 gggcttgaat acacaaactg aatgtggtca atttccatcc cttcctcctc atagccgatg
     1981 cttatataaa tgtaatcatc aacatcagcc agttcatagg tgttttcagc atcaacaagc
     2041 tgtcgcgaca tattctgatc ataaaactcc gtcaaaataa attcgttcag atcgccgata
     2101 tgctgaatgc gcacactgtc tgtttctgct ttcagtgtat cggcatgttc tttgcgcatt
     2161 ttccttattg tcctgcgctc gatacgggct ttttttcgct ccgcacgtct ttgcctgggt
     2221 gtcttaaaca tatcgctgcc ggatgcctca ttcaaactgt cagcattggc ctgccgaata
     2281 ctgtcataca tttgccgggg ctcatcacgc atcagcgata acttgcggtc gatgcggcat
     2341 ttgtatttgt cgagactccc aagatacaaa cggcgctttt ctcgcacctt ttgggcacct
     2401 ctaaaaactt gtttttttct tagtgatgcg atgcaaatat aagcgg
//
LOCUS       DAOPKE010000180         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_50141_length_2431_cov_12.144781, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000180 DAOPKE010000000
VERSION     DAOPKE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50141_length_2431_cov_12.144781"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1003
                     /locus_tag="PKY63_12735"
     CDS             89..1003
                     /locus_tag="PKY63_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPF01525.1"
                     /translation="MNTQSKLQKKSFTIVEQACHEVRGFRNLLQELDDKVRLSGHSAS
                     TLQNYSRKLAALSLHFGKLPQNISDKEVNKYLAQLARQSKTPSLSDFKFTVFGLRYCY
                     RLLGMTDHVVTLPQIKHTNKLPVVLNYSECRALFAAPELLKHRVMLSLIYSAGLRARE
                     LSKLKLADIDSERMMIHIRQSKYNKDRYIPLSPLILDGLRKYCRACNPVEYLFNGNQS
                     GEPLSVRGMQWVLKETVKKCKLQKGITLHTLRHSYATHLLEYGMDIVTIKELLGHERI
                     ETTMVYLHVAKPNRTNLFSPFDRLYNKD"
     gene            1000..2157
                     /locus_tag="PKY63_12740"
     CDS             1000..2157
                     /locus_tag="PKY63_12740"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HPF01526.1"
                     /translation="MKPAFELAQIIDRFGNEFVQQHTPNAYVQRTLRAIQQCRTAALG
                     GHVDQCDTCGHIRISYNSCRNRHCPKCQNTQREAWIEDRKADLLPVPYFHVVFTVPDS
                     LNALFLHRAQILYNILFRAAWETIAQFAFSSLNAETGMIAVLHTWGQNLSLHPHLHCI
                     VPGGGVDHKNQWKQVRMSANGKVFLFPVKNMSMVFRGKFLRQLQKVLPQEKSFIAEMY
                     KNNWVVYAKEPFAGPQQVVEYIGRYTHKVAISNHRLLSVDEKNISFRYLDYRDGKNKI
                     MQLDGAEFLRRFCMHILPKNFVRIRHYGILSSTGRKRFSELRIAFGLLPVSKRTKKDW
                     KQICREQLNYDPDICPHCQKGILKMIDSWSPGRPPPAPLSQIKTHQKNFQN"
BASE COUNT          710 a          505 c          518 g          698 t
ORIGIN      
        1 tgttgcacta aatcccgccc ctttcggagc gggataatca ttagtgtgtg tttttcgtat
       61 aaaggttttt tccacaaaat cttttattat gaacacacag tcaaaattac aaaaaaaatc
      121 gttcaccatc gttgaacaag catgtcatga agttcgtggt tttcgtaatc ttctacagga
      181 acttgatgat aaagtacggc tttcagggca ttcggcaagt acattacaga actattcacg
      241 gaaactggcg gcactgagcc ttcattttgg caagctacct cagaacatca gcgacaagga
      301 ggtaaataaa tatctggcac aattggcccg gcaaagcaag actccatcgc tgagtgattt
      361 taaatttact gtttttggtc ttagatattg ttatcgattg cttggaatga ctgatcatgt
      421 ggtcacattg cctcaaatca agcacacaaa taagttgccg gtggttctta attattccga
      481 atgccgtgca ctgtttgcag cacctgaact actgaagcat cgggtaatgt tatcgttgat
      541 atattcagct gggttgcggg cccgcgagct gagtaaactg aaattggcag acatcgattc
      601 agaacgaatg atgatacaca taaggcaaag caagtataac aaggatcgat acataccgct
      661 ttctccgctg attttggatg gtttgagaaa atactgtcgt gcatgtaatc cggtagaata
      721 tcttttcaat ggcaatcaga gtggtgaacc gttgagtgtt cgcgggatgc aatgggtgct
      781 gaaagagact gtcaaaaaat gcaaactaca gaaaggcata acgttgcaca cattgcgtca
      841 tagctatgct acacatttgc ttgagtatgg aatggatatc gtaaccatta aagaattact
      901 cgggcatgag cgaatcgaaa caacgatggt atatctgcat gtagctaaac ccaatcgtac
      961 aaacctgttc agcccgttcg acaggttgta taacaaagat tgaaaccggc ttttgagctg
     1021 gctcagatta ttgaccggtt tggaaacgag tttgttcagc agcacacgcc caatgcttat
     1081 gtgcaacgca ccttgcgagc catacagcaa tgtcgcactg cggctttggg tggtcatgtt
     1141 gaccaatgcg atacgtgcgg gcatattcgc atcagttaca acagttgccg caaccgtcat
     1201 tgtccaaaat gccagaatac acaacgcgaa gcgtggatag aagatcgcaa ggctgatttg
     1261 ttgcctgttc catattttca tgtggtattt accgtgccgg atagtcttaa tgcattgttt
     1321 ctgcacagag ctcagattct gtataatatt ttgtttcgtg ccgcatggga aaccattgct
     1381 cagtttgctt tcagcagcct caatgccgaa accggaatga ttgcagtttt gcatacatgg
     1441 ggacaaaatc tgagtctcca tccgcatttg cactgtattg tgccgggtgg cggtgttgac
     1501 cacaaaaacc aatggaaaca ggttcgcatg tcggccaatg gaaaagtttt tctctttccg
     1561 gtaaaaaaca tgtcgatggt ttttagaggt aagtttctgc gccagttgca gaaggttctt
     1621 ccgcaggaaa aatcatttat tgctgaaatg tacaaaaaca attgggtggt gtatgccaaa
     1681 gaaccttttg ccggaccgca gcaggtagtc gaatatatag gtcggtacac acataaggtt
     1741 gccattagta atcatcggct tctttcagtt gatgaaaaaa atatttcttt cagatatctt
     1801 gattaccgcg atgggaaaaa caaaatcatg caacttgatg gcgcagaatt tttacgacga
     1861 ttttgcatgc atatattgcc aaaaaacttt gtcagaatca ggcattacgg cattttgtct
     1921 tctaccggac gaaaacgttt ttctgagctt cgaatcgctt ttggtctgct cccagtctcg
     1981 aaaagaacaa aaaaagattg gaaacaaatc tgccgtgaac aattaaatta tgaccccgac
     2041 atttgtccgc attgtcaaaa aggaatactg aagatgattg actcctggtc gccaggtagg
     2101 ccgcctccag ctcctctttc tcaaataaaa acacatcaaa aaaattttca aaattaatca
     2161 atgtcaaata tgaccctggc cgcgcattgc tatattattc aaaacaaacg actgaatgca
     2221 gcttgtaacc tgcaactctc cttatatttt tgttcatgtt ttctctttct cagtaaaaaa
     2281 acaatgaggt cttcagatct caatattgca ttttctaaat aaatttccat aagtaactca
     2341 gtgtgctcaa tcattgtcgc aaggcggcat ttcgtgcaac atggcttcat gtttggcttc
     2401 gtccctcagc cacacgaagc cttagttatt a
//
LOCUS       DAOPKE010000181         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_50513_length_2419_cov_13.634518, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000181 DAOPKE010000000
VERSION     DAOPKE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50513_length_2419_cov_13.634518"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKY63_12745"
     CDS             <1..414
                     /locus_tag="PKY63_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF01527.1"
                     /translation="TMRVTEQIDALDVMGINSANFLIFPKIVAFVLIFPVLVIISMFL
                     GVMGGYMVAVLTDLFSVETYMLGVRSFFIPFEVFYSLIKSLFFAFIITSVSSYFGYTV
                     NGGSLEVGRASTKAVVQSSIMIILFNVILTQILLT"
     gene            411..1163
                     /locus_tag="PKY63_12750"
     CDS             411..1163
                     /locus_tag="PKY63_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF01528.1"
                     /translation="MIEVRNINKSFEGKQVLKNISVKLEPGINNLIIGQSGSGKTVLL
                     KCLLGLHKVDNGEIFFDERNFTVMNEYDQKKIRQEIGTVFQGGALFDSMTVEENVMFP
                     LNMFTNQTRQQKKERVNFCLRRVDLQHSNKLMPSEISGGMKKRVAIARAIVMNPKYLF
                     CDEPNSGLDPKTSILIDQLINELTREFNMTTVINTHDMNSVIEIGDNVAFIYKGNLWW
                     NGSNKDILHTDNKELNDFIFATQLTRHLKGKL"
     gene            1160..1687
                     /locus_tag="PKY63_12755"
     CDS             1160..1687
                     /locus_tag="PKY63_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPF01529.1"
                     /translation="MNLIDIVLLIPIGWGIYKGFSKGLIHELAQIAALVLGVLAGLYL
                     SKWIGGFIAGIFGTSEEHTQLIAFLVAFLGTLVLVFLVARIAENAVKNMSLGIVNRII
                     GAAFASIKFILILSLIINFINSTDKHGLFLKKETREGSLLYKPVGAVAPLVMPFIMQV
                     MSETEDETTVSKPED"
     gene            complement(1807..2031)
                     /locus_tag="PKY63_12760"
     CDS             complement(1807..2031)
                     /locus_tag="PKY63_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01530.1"
                     /translation="MKEKVLKPIIFFVVLISSINLSGQDYEHITGLGTSFTDAAQKYW
                     YYRDRLKYFVMPGTEPGQSIVFTHRNDNYP"
     gene            complement(2034..>2419)
                     /locus_tag="PKY63_12765"
     CDS             complement(2034..>2419)
                     /locus_tag="PKY63_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01531.1"
                     /translation="RVTGFEKNGVSVLEEWRSQYDWRLTFFGKESDHYFTATNCNCYI
                     DTADVTIRTADGKWMFGNDNYTRLNFAFGFHYGTDKTCIGMGMYPLISGYGLHFDVLK
                     LSNSKLWLNTTDSLDNDYLLKFEKE"
BASE COUNT          698 a          534 c          479 g          708 t
ORIGIN      
        1 accatgcggg ttacagagca aatcgacgcg cttgatgtca tgggaatcaa ttccgcgaat
       61 tttctgattt tcccaaaaat tgtggctttt gtgcttattt tcccggtact ggttatcatc
      121 tccatgtttc ttggggttat gggcggatac atggttgcag ttctcaccga cctcttcagc
      181 gttgaaacat atatgctcgg agtacgatcg ttctttattc cgttcgaagt gttctactcg
      241 ctcatcaagt cattattctt tgccttcatc attacttccg taagttcgta tttcggatat
      301 acagtcaacg gcggctcgct cgaagtcggc cgtgcaagca ccaaagcagt ggttcagagc
      361 agcatcatga ttatcctctt caacgtcatt ctcacccaaa tcctgctcac atgatcgagg
      421 tacgcaacat caataaaagc tttgagggga agcaggtgct gaaaaatatt tcagtaaaac
      481 ttgaacccgg gattaacaat ctgatcattg gtcaaagcgg atccggaaag actgttcttc
      541 tgaaatgcct gctgggactg cacaaagttg ataacggaga gatttttttc gacgagcgga
      601 attttacggt gatgaatgaa tacgatcaga aaaaaatccg tcaggaaata ggaacggttt
      661 ttcagggcgg cgctctgttc gattccatga cagttgaaga aaatgtaatg ttcccgctca
      721 atatgttcac caaccaaaca cggcagcaaa aaaaggaacg ggtgaatttc tgcctccgca
      781 gagtggatct gcagcattca aacaagctga tgccttcgga aatcagcggt ggtatgaaaa
      841 agcgtgtggc cattgcacga gccattgtca tgaatccgaa atatctcttc tgcgacgaac
      901 ccaactctgg tctcgatcca aaaacatcca tcctgattga ccagctgatc aatgaactca
      961 cccgcgaatt caatatgacc accgtcatca atacacatga catgaattcg gtgattgaaa
     1021 tcggcgacaa tgtggctttc atttacaagg gaaacctttg gtggaacggc tccaacaaag
     1081 acattcttca tactgacaac aaagagctga acgattttat ttttgcaacc cagttaaccc
     1141 gtcacttaaa aggaaaattg tgaatctgat tgatattgta ctgctgatcc ccattggctg
     1201 gggcatttac aaaggttttt cgaaaggatt gattcatgaa ctggcacaga ttgccgcact
     1261 tgttctgggt gtgcttgcag gactttatct gagtaagtgg attggtggtt tcattgccgg
     1321 tattttcgga accagcgaag aacacaccca gttgattgcc tttctggtgg cctttctcgg
     1381 aacactcgtg ctggtgtttc tggtggcccg cattgccgaa aacgccgtaa aaaacatgag
     1441 ccttggaatc gtgaaccgca ttatcggtgc agctttcgca agcatcaagt ttattcttat
     1501 cctcagcctg ataatcaatt ttatcaactc aaccgacaaa cacggacttt ttctgaaaaa
     1561 agagacccgc gaaggaagtc tgctctacaa gcccgttggc gctgttgctc cgctggtaat
     1621 gccctttatc atgcaggtga tgtcagaaac cgaagatgaa actacagttt ctaaaccgga
     1681 agattgaaga ggaataattt taacaatctg atgttttcgg aaaacaacaa cttcttcaaa
     1741 aacaatattc catcgtcctg aatcgacgaa ggcgcagaaa gatataaaat cgttgttttt
     1801 gaaacattac ggatagttgt catttctatg cgtgaataca atactttgcc cgggctctgt
     1861 tcccggcatt acaaagtatt taagtctgtc tctgtaatac cagtattttt gagctgcatc
     1921 tgtgaaactc gtccctaatc ctgttatatg ttcataatct tgtcctgaaa ggttaatgct
     1981 actaataaga acaacaaaga agatgatcgg tttcaatact ttttctttca tatttattcc
     2041 ttttcaaatt tcaacaaata atcgttatcc agcgagtctg ttgtatttag ccataatttt
     2101 gagttagata actttaatac atcaaaatgt aacccatatc cagaaattaa tggatacatt
     2161 cccatcccta tgcaagtttt atctgttcca taatggaacc caaatgcaaa gtttaaacgc
     2221 gtataattgt catttccaaa catccattta ccgtcagctg tccgtatagt cacatcagca
     2281 gtatcaatat aacaattaca atttgttgct gtaaaataat gatcactttc tttgccaaaa
     2341 aacgtcaatc tccaatcgta ttgcgatcgc cattcttcca aaacactgac accgtttttc
     2401 tcaaaaccgg ttactctcc
//
LOCUS       DAOPKE010000182         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_50851_length_2407_cov_15.648810, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000182 DAOPKE010000000
VERSION     DAOPKE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50851_length_2407_cov_15.648810"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKY63_12770"
     CDS             complement(<1..448)
                     /locus_tag="PKY63_12770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPF01532.1"
                     /translation="MSNNENILITEAKKTLRRQMRDVKAAITTEEKKNKSTLIFEYIE
                     KQPWFINAKFVMCYWSLPDEVLTHDFCRKWMNKKIILLPRMTGPDIIPVMFDGTLAKE
                     PILGVEEPQGPEFSQIDMINIIIVPGVAFDASGNRMGRGKGYYDKFL"
     gene            complement(455..>2407)
                     /gene="dnaG"
                     /locus_tag="PKY63_12775"
     CDS             complement(455..>2407)
                     /gene="dnaG"
                     /locus_tag="PKY63_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPF01533.1"
                     /translation="RREDILRIMDAVRIEEVIGEYLSLKKRGSNLIGLCPFHNEKTPS
                     FNVSPSLNIFKCFGCGKAGNAVNFLMEYEHYTYPEALRKLAAKFGVEIVEEEVDDDYK
                     EKKTEEDQAYFVHEFAHKWFEKQLWETKEGMSVGLSYFHERGLSDAIIKKFGLGYSPN
                     NYTAFADHARNEGFDKETLAKNGLINGESGNDRFRDRIIFPIFSISGRVIAFGGRTLR
                     TDAKIAKYVNSPETPIYHKSNVLYGIHLAKNSIISQNECYLVEGYMDVIAMVAAGINN
                     VVASSGTSLTGDQIRMIRRYSSNVCILYDGDMAGIKASFRAIDMLLAEGLDVRVVLFP
                     DKEDPDSYSRKYSAKALMDFLENNKKSFVLFKAEIMFADAGNDPLLRAGAMKDIVHSI
                     AEIPDGISRSLLVTECAKLFNIEEKTLMFEMNKMLRAKAKKLLRPEDESGVEFEEESP
                     EKQQAAIGTDRQEEAEKNILRMILLFGDKTMRFQVKNPELKITEIVEKTVAHYVFEEL
                     EADSMKLSIPKLLRIYDICKIQAQTDGISNASRVLNMLDPLLTNTTADIMNTGYSLSP
                     NWEKKHGIYIQHAENDNSILISEVDSAILEFKHLMITREMDAIRKKLQEENDEENVIL
                     LVGQFQLYKTIDVEIEKRIRQRVINK"
BASE COUNT          559 a          552 c          513 g          783 t
ORIGIN      
        1 gtagaaattt gtcataatat cctttccctc ttcccatgcg gtttccgctt gcatcgaatg
       61 cgacgccggg aacgatgatg atatttatca tgtcaatctg actgaattcc ggcccttgtg
      121 gttcttcaac acccagaatg ggttcttttg ccagggttcc gtcgaacata accggaataa
      181 tgtctggtcc ggtcatccgc ggaagcagaa tgattttttt attcatccat ttccgacaaa
      241 aatcgtgtgt tagaacttca tcgggcaagc tccagtaaca catcacaaat ttcgcattga
      301 tgaaccaggg ttgcttttcg atgtattcaa aaattaatgt cgatttgttc tttttttctt
      361 cagtagtgat tgctgctttc acatccctca tctggcgccg gagagttttt tttgcttcgg
      421 taatcaatat gttttcattg ttactcattg cgcactattt gttgatgacc ctctgccgga
      481 ttcgtttttc aatttcaaca tcaatggttt tatatagttg aaactgaccc accagcagta
      541 ttacattttc ttcgtcattt tcttcctgca gtttttttct gatagcatcc atttcgcgtg
      601 taatcatcag atgcttgaat tcgagaatgg cgctgtcaac ttcgctgatg agaatactgt
      661 tgtcgttttc ggcatgttga atataaattc cgtgtttttt ctcccagttt ggactgagtg
      721 aatatcccgt gttcataata tcggcagttg tattggtcag gagtgggtcc agcatgttta
      781 aaacacgact tgcattgctt attccatcag tttgtgcctg aattttgcag atatcataaa
      841 tacgcaggag ttttgggatt gacagtttca ttgaatcggc ctcgagttct tcaaaaacat
      901 aatgagcaac cgttttttca acaatttctg tgattttcaa ctcgggattt ttcacctgaa
      961 atcgcatggt tttatcgccg aaaagaagaa tcattcgcag aatgtttttt tcggcttctt
     1021 cctgcctgtc ggtgcctatg gctgcttgtt gtttttcggg ggactcttcc tcaaattcaa
     1081 cgcctgactc atcttcgggt cgcagcagtt tttttgcttt agcgcgaagc attttgttca
     1141 tttcgaacat cagcgttttc tcttcaatgt tgaagagttt tgcacattcg gttaccagca
     1201 gtgaacgcga tattccatca gggatttcag caatgctgtg tacaatatct ttcatcgctc
     1261 cggcgcgtag cagcggatca ttgccggcat cggcaaacat gatttcggct ttgaacaaca
     1321 caaatgattt tttgttgttc tccagaaaat ccatcagcgc tttggcggaa tattttcgcg
     1381 aataggagtc cggatcttct ttatctggga ataataccac gcgcacatcc agcccttcgg
     1441 ccagcagcat atcaatggcg cggaacgaag ctttgatgcc ggccatatcg ccatcgtaca
     1501 aaatgcacac attgcttgaa tagcggcgta tcatgcggat ttggtcgccg gtaagactgg
     1561 tgccgctcga tgccaccaca ttgttgattc cggctgcaac cattgcaatc acatccatgt
     1621 agccctctac cagataacat tcattctggc tgatgatgct gtttttggct aaatgaattc
     1681 cgtaaaggac attgcttttg tgatatattg gtgtttccgg cgagttgaca tatttggcaa
     1741 tttttgcatc ggtgcgcagg gtgcgtcctc caaaggcaat tacacgcccg ctgatgctga
     1801 aaataggaaa tatgatgcga tccctgaaac ggtcattgcc gctttcgccg ttgatgagcc
     1861 cgtttttggc cagtgtttcc ttgtcaaatc cttcgttgcg ggcatgatcg gcaaaagctg
     1921 tataattgtt tggagaataa ccgagaccga atttttttat gatggcatcg ctcagcccgc
     1981 gttcgtgaaa ataactcagc ccgaccgaca ttccttcttt cgtctcccag agctgctttt
     2041 cgaaccattt atgtgcaaat tcatgcacaa aataggcctg atcttcttcg gtcttttttt
     2101 ccttgtaatc gtcgtcaact tcttcttcaa cgatttccac tccaaatttg gcagccagct
     2161 tgcgaagcgc ttcgggatac gtataatgct cgtactccat cagaaaattc actgcgttgc
     2221 cggctttgcc gcagccgaaa catttgaaaa tattcagaga gggcgataca ttgaacgaag
     2281 gtgttttttc gttgtggaac ggacataatc cgatcagatt ggaaccgcgc ttctttagcg
     2341 acaggtactc tccaatgact tcttcgatgc gcacggcatc cataatgcgt aatatgtctt
     2401 cgcgacg
//
LOCUS       DAOPKE010000183         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_51143_length_2398_cov_10.593683, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000183 DAOPKE010000000
VERSION     DAOPKE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51143_length_2398_cov_10.593683"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1166)
                     /locus_tag="PKY63_12780"
     CDS             complement(69..1166)
                     /locus_tag="PKY63_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPF01534.1"
                     /translation="MRLKSLKLNQFKNYEEVSLNFTGNVHCFYGNNGAGKTNILDAIH
                     YLSFTKSYFGGSDQNCIRFGYEFFAINGEFEMPSGVSEKVSVSVKSGGEKKVKRNDKE
                     YQRFSEHIGAFPVVMITPGDIDLINNAAEVRRRFFDAVISQFDKTYLDDVIRYNKVLM
                     QRNKALKEMGERGRGDFNDLEVWNAQLVNYGQRIHEMRGKYQSDMRVFFDQMHGRLTG
                     NGDNAEIIYESMLNERDMSDILRDSFRNDFGAGFTTQGIHRDDYIFMVNENPVRRFGS
                     QGQQKSFLLSLKMAQYLITVDKTGMTPILLFDDIFDKLDPERVRLLIGMVCHEPFGQV
                     FITDTHKMRMEDIIGHEAQIQLFEVKDGGVV"
     gene            complement(1163..>2398)
                     /locus_tag="PKY63_12785"
     CDS             complement(1163..>2398)
                     /locus_tag="PKY63_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPF01535.1"
                     /translation="ILTIGDELLIGQVIDTNSAWLAMRLNELGFTIKARFSVGDNMSE
                     IRDAIYYATELAPVVITTGGLGPTSDDRTREVLCDMFECEMQENEEVLNGIKDMFAKR
                     GWPLTDVNRRQALVPDRAKVLFNKMGTAPGLYFEDGSTHIFVMPGVPFEMKEMFASAV
                     VPHLKKVSGHQAYVHETRVFSGIGESWLSDRIKPWEEKLPKNVSLAYLPAPGIIRLRL
                     SHLADNEIEGRALLTQLFADVMPLVNDYFVSNIDESIAATLLREMTESGKTICSAESC
                     TGGYIAHQITSIPGSSAAFKGSVVAYSNEIKEKILNVSAATLLQFGAVSNQTVCEMAV
                     NARKILNTDYAMAVSGIAGPDGGTAEKPVGLVWIAVASDIGVESQELKLGGPRDVIIA
                     RSAVSAMFMCLKEKRKDKK"
BASE COUNT          646 a          616 c          471 g          665 t
ORIGIN      
        1 ctactcttaa caacttactc aaagaccatt ttttattgca tggatacttt caacttatag
       61 cgctcgcgct ataccacgcc gccgtctttc acttcaaaga gctgtatctg tgcttcgtgg
      121 ccaataatgt cttccattcg catcttgtgc gtgtcagtta taaacacctg cccaaacggc
      181 tcatgacaca ccattccgat gagcaaccga acccgctccg gatcaagctt atcaaatata
      241 tcatcgaaca ataaaatcgg cgtcatgcct gttttgtcaa cggtaatgag gtattgcgcc
      301 atcttgagcg acagcagaaa acttttttgc tgaccctgac ttccgaagcg gcgcaccgga
      361 ttttcattca ccataaaaat ataatcgtcg cggtgaatgc cctgtgtggt aaagccggct
      421 ccgaaatcgt tgcggaagct gtcgcgcaga atatcactca tgtcgcgctc gttgagcatc
      481 gattcataaa taatttcagc gttgtcgccg ttgccggtca aacgcccgtg catctgatcg
      541 aaaaacacac gcatgtcgct ctgatacttg ccgcgcattt cgtgaatacg ctgaccgtaa
      601 ttcaccaatt gtgcattcca gacttccaga tcattgaaat cgccccgacc acgttcgccc
      661 atttctttca atgctttgtt acgctgcatc agcactttat tgtagcgtat cacatcatca
      721 aggtaagttt tatcgaattg cgaaatcact gcatcgaaga aacgacgacg gacttcagca
      781 gcattgttga tcaggtcaat atcgcccgga gtaatcataa ccaccggaaa agcgccgatg
      841 tgttccgaaa agcgctggta ttccttatcg ttgcgtttta cttttttttc gccacccgat
      901 ttcaccgaca ccgacacttt ttccgaaaca ccagaaggca tttcgaattc accattgata
      961 gcaaagaact catagccaaa gcggatacag ttctgatcgc tgccaccaaa ataacttttt
     1021 gtgaaactca gataatgaat ggcatcgaga atattggttt tccctgcccc attgtttcca
     1081 tagaagcaat gcacgtttcc ggtaaagttc agagaaacct cttcgtagtt tttaaactga
     1141 tttaatttca gtgattttaa tctcatttct tgtcctttcg tttttctttc aaacacataa
     1201 acatggccga gaccgcactg cgggcaataa tcacatcgcg cggaccaccc agtttcaatt
     1261 cctgcgattc aactccaatg tccgaagcta cagcaatcca aaccaggcct acgggttttt
     1321 cagcagtacc gccatctggt ccggcaatgc cgctgacagc catagcataa tcggtattca
     1381 gaatctttcg ggcattaacg gccatttcgc aaaccgtttg attactcaca gcaccaaatt
     1441 gtagtaatgt agctgcgctg acatttaaaa ttttttcttt tatttcgttg gaatatgcta
     1501 ccacactgcc tttgaatgca gccgagcttc cgggtatcga agtaatttga tgagctatat
     1561 agccaccagt gcaactttcg gcagaacaaa ttgtttttcc ggattctgtc atttcacgca
     1621 gcaaagttgc agcaatggat tcatcaatat tagatacaaa ataatcattc accagcggca
     1681 tgacatcagc aaataattgt gtcagcaatg ccctgccctc tatctcgtta tcggccaggt
     1741 gactcagccg cagacgaatg ataccaggtg caggtaaata tgcaagactc acatttttgg
     1801 gcaatttttc ttcccatggt ttaatgcgat cgctcagcca cgattcacca ataccgctga
     1861 atacacgggt ttcatgaaca taggcctgat gtccggacac ttttttcaaa tgcggtacaa
     1921 cagccgatgc aaacatttcc ttcatttcga aaggaacacc cggcataaca aaaatatggg
     1981 ttgagccatc ttcaaaatac agaccaggag cggttcccat cttattaaac aatactttgg
     2041 cgcgatcggg aaccaatgcc tgccggcggt tcacatcggt caacggccag ccacgttttg
     2101 caaacatatc tttaatgcca ttcaacactt cttcattttc ctgcatttcg cattcgaaca
     2161 tatcgcacaa aacttcgcgt gtacggtcgt ccgatgttgg accaaggccg ccggtagtaa
     2221 taacaaccgg agcaagctct gttgcataat aaatggcatc gcggatttcg gacatattat
     2281 cgccaacgct gaagcgtgct tttatggtaa atccaagttc gttcagccgc atggcaagcc
     2341 atgccgagtt ggtatcgatg acctgaccaa tcagtaattc atcgcctatt gtgagtat
//
LOCUS       DAOPKE010000184         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_53733_length_2317_cov_14.854111, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000184 DAOPKE010000000
VERSION     DAOPKE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53733_length_2317_cov_14.854111"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..2054
                     /gene="rho"
                     /locus_tag="PKY63_12790"
     CDS             186..2054
                     /gene="rho"
                     /locus_tag="PKY63_12790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPF01536.1"
                     /translation="MQEKPDLNKMLLPELREYAATLNIKGISTMRKPELLRQINEKLS
                     GIIPTEEKSSSVVELFSNDAIISDEKPFVSDNHETEQKRKRIEQSEKQESVVPPAIVI
                     NPVISEKSDEDSSLKPAPEADKQQDGTFPVHNKFRKDFKNGGDFTFKKKKPWQREGFV
                     PVNKNTNQPSNNQQQNNQSKQYPKDDILLPVEPFEEVHDEQLAMQENNLEQNEKQVAA
                     NVQPSVNERPVHQHNQQQHPQQRQEKPAPFFELDGVVTAEGVLELVPEGFGFLRSSDY
                     NYLNSPDDVYVSQNQVKMFGLKTGDTVECTIRPPKDGEKYFTSVKIVNVNGLEADKIR
                     DRIPFDFLTPLFPIKKFKLTGHPEETLTSRVIDLFSPIGKGQRGLIVAQPKTGKTVIL
                     KELANAIAYNHPEAYLIILLVDERPEEVTDMQRSVRAEVIASTFDEPADKHVRIANIV
                     LEKAKRMVECGHDVVILLDSITRLARAYNTVAPASGKVLSGGVESNALQKPKRFFGAA
                     RQIENGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMELQLDRKLSNKRVYPAIDIVA
                     SSTRRDDLLVAKDVLQRVWIMRTHLADMNPLEAMEFLQERMKFTKTNEEFLMTMNS"
BASE COUNT          723 a          447 c          526 g          621 t
ORIGIN      
        1 catttgttca gcgtatagat aataaaaagt tcattcctga aaaatcgaaa aatacgtgaa
       61 aatattatca tctttttcaa aaccgttgca tagttgaaat tatgttgtat atttgctgca
      121 tcaaaatgga tttacacatc ctcctgttat tttagtatct cccaaattta cttaacacct
      181 cctacatgca ggaaaagcca gatcttaaca agatgctgtt gcccgaatta cgtgagtatg
      241 cagctacatt gaatattaaa ggtatatcaa ctatgcgcaa gccggaattg ctgcgccaga
      301 taaatgaaaa attatcagga attattccta cggaagaaaa atcttcttcc gtggtggaat
      361 tgttttctaa tgatgccatc atttcagatg agaaaccatt tgtttcagat aatcatgaaa
      421 ctgaacaaaa aagaaaaaga attgagcaaa gcgaaaagca agaatctgtt gtgccgccgg
      481 caatagttat taatcctgtt atatcggaaa aatccgatga agacagctct ttgaagccgg
      541 ctccggaagc cgataaacag caggacggaa cctttccggt gcacaataaa ttccgtaaag
      601 attttaaaaa tggtggtgat tttactttta aaaagaaaaa accgtggcag cgtgaaggat
      661 ttgtgccggt taataaaaat accaatcagc cgtcgaataa tcaacaacaa aacaaccagt
      721 cgaaacaata ccccaaagac gatatacttt tgccggttga gccattcgaa gaagtgcatg
      781 atgagcagct tgccatgcag gagaataatc tggaacaaaa tgaaaagcag gttgcagcca
      841 atgtgcagcc atcagtgaat gaacgtccgg ttcatcagca caaccagcag caacatccac
      901 agcaacgaca ggaaaaaccg gctccttttt tcgaacttga tggagtggtt acggcagaag
      961 gagtgctgga gctggttcca gaaggttttg ggtttttacg ttcatcggat tataattatt
     1021 tgaattctcc ggacgatgta tatgttagcc agaatcaggt aaaaatgttt gggctgaaaa
     1081 caggtgacac agtagagtgt accattcgtc cgccgaagga tggtgaaaaa tattttactt
     1141 ctgtaaaaat tgtaaatgtc aatggtttgg aggcggataa aatccgcgac cggattcctt
     1201 ttgatttttt gacaccgttg tttcctatta aaaaattcaa gcttacagga catcctgaag
     1261 aaactctgac aagccgtgtt attgatttgt tttcacccat tggaaaagga cagcgtgggt
     1321 taattgttgc gcaaccaaag accggtaaaa cggttatact gaaagagctt gccaatgcca
     1381 ttgcatacaa tcatcctgaa gcatatctga ttattttgct ggtggatgag cgtcccgaag
     1441 aagtgaccga tatgcagcgt agcgtccgag ctgaagttat tgcatcaacc tttgatgaac
     1501 ccgcagataa acatgtccgt atcgcaaata ttgttttgga aaaagcaaaa cggatggtgg
     1561 aatgtggtca tgatgtagtg attctgctcg actccatcac acgtctggcg cgcgcttaca
     1621 atacagtggc cccggcttcc ggaaaagttt tgtcgggtgg tgttgaatca aatgctttgc
     1681 aaaaaccaaa acgtttcttt ggagctgccc gtcagatcga aaacggcgga tcgctcacca
     1741 tcattgctac ggcgctgatt gacaccgggt cgaaaatgga tgaagtgatt tttgaagaat
     1801 tcaagggaac cggtaatatg gaattgcagc tcgatcgtaa attgtcgaat aagcgggtgt
     1861 atcctgccat agatattgtt gcatccagta cgcgacgtga tgatctgttg gtagcaaagg
     1921 atgtactgca gcgtgtttgg attatgcgta ctcacctggc cgacatgaat ccgctcgaag
     1981 caatggagtt tctgcaggaa cgcatgaaat ttaccaaaac caacgaagag tttctgatga
     2041 cgatgaactc gtagaaatac gagattagag atacgaaagg atgttccgct tacagcggaa
     2101 taaattccaa tgtgcgtctt cggcgtgaaa cgcgacacca gtcggcgatg tgacgccttc
     2161 acgtgaaacc acgtaaaacg tggcaagctg tgataaatcg gcgatgtgtg aatattagat
     2221 tgtcattttt gtcccttcga caggctcagg acagagcttt ttataccatc gtgaaatata
     2281 ttttattcca atgtgtctaa gtgcagattg atggctg
//
LOCUS       DAOPKE010000185         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_53935_length_2311_cov_10.653369, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000185 DAOPKE010000000
VERSION     DAOPKE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53935_length_2311_cov_10.653369"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..2024)
                     /gene="argS"
                     /locus_tag="PKY63_12795"
     CDS             complement(246..2024)
                     /gene="argS"
                     /locus_tag="PKY63_12795"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPF01537.1"
                     /translation="MFTQLLAQATSDIASQLWNQQVAPAFIQIEKTPSHFEGDVTIVV
                     FPFVKMARLKPEDTAQAIGAALSEKISGIDRFNVVKGFLNITFTPAFWLSQMSEVAPV
                     AEYSRPAGVEPKTIVLEYSSPNTNKPLHLGHIRNNLLGWSLAEVYKAAGHNVIKANLI
                     NDRGIHICKTMVAYNMWGNNETPESAGVKGDHLIGKYYVLFDKANKEEAAELGIEKPE
                     DGDTPLMKDARAMLRDWEKGEPHVMALWNKLNSWVIDGFNDTYARLGVDFDKVYRESE
                     TYLSGKDIVQKGLAEGHLQQDPDGSVWIDLTDEGLDRKILLRSDGTTVYMTQDIGTAV
                     ARHNDFGADKMVYVVGNEQIYHFDVLKKTLKRLGYDWADNILHYSYGMVELPEGKMKS
                     REGTVVDADDLLDEMKQTAADISKEAGKAQDLPAVEQDKLHEMIGQGALKYFILRVDP
                     KKNMLFNPSESIDFNGNTGPFIQYTHARICSVLNKGREAGIAVDNLKWSADADLKPQE
                     KELLYQLLTLPQMTEKAATNESPAVVANYSFELAQMFNSMYQEISILREPDETLRNNR
                     LLLAKRTAETLKFAMGLLGVGMPEKM"
BASE COUNT          576 a          578 c          492 g          665 t
ORIGIN      
        1 aaaaaatcat ccaccgcatt ttcaaaatcg atgtcatcag catcgaaaca gcgataatga
       61 gcgatccggt gtatccgatc agctcggcaa tggtcattcc tgcaattaaa atctctgtgt
      121 ccatttatat tatgtcttat gaaacctgaa gccctttttt ttgttgtcag tagccagtag
      181 ccagtgaaaa ctctcactca taacttatca ctcataactc acaactgact actgaccact
      241 gcaaatcaca ttttctccgg cattcctacg cccagcaatc ccatggcgaa tttcagcgtt
      301 tcggctgtgc gctttgcaag caacaaacga ttgttgcgca gtgtttcatc gggctcgcgc
      361 agaatcgaaa tttcctgata catactgttg aacatctgcg ccagctcaaa gctgtaattg
      421 gccaccacag ccggactttc gtttgtcgcc gccttttcag tcatctgcgg aagcgtgagc
      481 aactgataca acaattcttt ttcctgaggc tttaaatccg catcagcaga ccatttcaaa
      541 ttgtcaacag caattcctgc ttcgcggcct ttgttcagaa cggagcaaat ccgtgcatgc
      601 gtgtactgaa tgaagggccc tgtgttgccg ttaaaatcaa tgctttcgga aggattgaac
      661 agcatgttct ttttcggatc gacacgcaga ataaaatatt tcaatgcgcc ctgtccgatc
      721 atttcatgca gcttgtcctg ttctacagca ggcagatcct gggctttacc ggcttctttc
      781 gaaatgtcgg cagcggtttg tttcatttca tcaagcagat catcggcatc aaccacagtc
      841 ccctcgcgcg atttcatctt tccttccgga agttccacca tgccgtagct gtagtgcagt
      901 atattgtcag cccagtcgta gcctaatctt ttgagtgttt ttttcagaac atcgaagtga
      961 tagatctgtt cattgccgac aacatatacc attttatcag caccgaaatc gttgtggcga
     1021 gctacagcag tgccgatgtc ctgagtcata tatactgtgg tgccatcgct gcgcaaaaga
     1081 attttccggt cgagtccttc atctgtcaga tcgatccaca ccgagccatc gggatcctgc
     1141 tgcagatgtc cttccgcaag ccctttctgc acgatatcct ttccgctcag ataggtttcg
     1201 gattcacggt acaccttatc gaaatcgacg cccagtcttg cataggtatc gttgaacccg
     1261 tcaataaccc agctgttgag tttgttccac aacgccatta catgtggttc acccttttcc
     1321 cagtcacgca gcatggctcg cgcatccttc atcagaggcg tatctccgtc ttcaggtttc
     1381 tcaatgccca gttcggcagc ttcttccttg ttggccttat cgaacagcac gtaatatttt
     1441 ccgatcaggt gatcgccttt tacgccggcc gattcaggtg tttcattgtt gccccacatg
     1501 ttgtaggcaa ccatggtttt gcagatatgt attccgcgat cgttgatgag attggctttg
     1561 atcacattat gcccggcggc cttatatact tcggccagcg accagcccag cagattgtta
     1621 cggatatggc ccaggtgcag cggtttattg gtattgggcg agctgtattc aagcacaatg
     1681 gtcttgggct caacacctgc aggtcggctg tattcagcca ccggagcgac ctccgacatt
     1741 tgagacaacc aaaaagccgg tgtaaaagtt atattgagaa atcctttcac gacattgaag
     1801 cggtcgatgc cggaaatctt ttccgacaaa gcagcgccga tagcctgagc ggtgtcttcg
     1861 ggtttcaggc gggccatttt cacgaaagga aatacaacaa tggtcacatc gccttcgaaa
     1921 tgcgacggtg ttttttctat ctgtatgaac gcaggcgcca cctgctgatt ccagagttgc
     1981 gatgcaatat ccgatgtggc ctgcgccaga agctgagtga acattcggtt tttgtttgca
     2041 aatttacaac ttttaagtct ttagcttttt aaagttagaa tccggaccga atttccccaa
     2101 gacagacaca aaatccggtt tcgtcgggat gcgaaaaaga ttaccagttc ggggtaaagc
     2161 tataagcaag tcgcccacac gcttatagtt gttcttaaca aattgcttat aaaatattcg
     2221 ttttaaaact tttgtagttc tgtttaaaaa tgtaaatttg cagcaaattc aaaacaaaac
     2281 gttgaacgat gaaaagaaat gttattatca t
//
LOCUS       DAOPKE010000186         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_55453_length_2267_cov_12.844937, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000186 DAOPKE010000000
VERSION     DAOPKE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55453_length_2267_cov_12.844937"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(859..2118)
                     /locus_tag="PKY63_12800"
     CDS             complement(859..2118)
                     /locus_tag="PKY63_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF01538.1"
                     /translation="MEKSRLKEIMLDQKESFNRRSALTERDIDLRKAISTKQIVIVSG
                     IRRCGKSTLLYFIKEKMKLKESEYCYFNFDDERIVADVSIFEDLYNLHLELYGIEPVL
                     FLDEIQNILHWERFVNRMYERGNKIFISGSNANLQLSDVAASLTGRNKQIEMFPFSFV
                     EYLTFMGHTYDLSGMTTKARSLLNLDFNHFLSMGGFPLVLKEDDLELINAYFSDILYR
                     DIVARYRLSQVSEIKEIGLYFASNVSKVFSYSTLQQISGVKSLSSVKDYLFYYEQAHL
                     FHYVRKFDYSLKKQVMNPRKVYVADQAFAHRLGFNFTANTGRILENIIYLELLRRGKE
                     VFYYSGKNECDFLVRDGLKITEAIQVVHQANSSNIKREVDGLREAMETFKIPKGLIIT
                     SDSSHSAFPDIKGVRIIDAPHWLLENK"
BASE COUNT          602 a          546 c          444 g          675 t
ORIGIN      
        1 cttgataaac ttttaactac caaattcccg gtccccgatt ttgaataatg aacaatccgt
       61 gcattctgac ggatcaagag gaacaaggaa attaaaagta cgaagtaagt ttgatttata
      121 aactccttca acattctcac ttccttgcct gcctggccgg cagacaggtt cagtgttcag
      181 agttctttgg gtgccgggat ctcccggaaa accgcctcag cttttccact ttcgctgtcc
      241 acgaattatt cactcattat ataattttta tcggtgttca tgagctcagt gcctttcagg
      301 cacttcggtt acacgaattc cacgaattat caacccctct gctggcacct ctgattttag
      361 cgaataacta cgctttcatc tgcgatgata gcgtagttca cgaatgaata agctgaaact
      421 gattgcttcc tatttttttt ggcttggtgc ctttgtggca aatcccccac cttgggcgcg
      481 cgtctccgcg tgcaacgttt cacgttgacg cgtgaccttc catttcaaag gagcttcacg
      541 taaaacgtga caggtcgcac accttgtccc tggtgcaggc gcccaaggct aacatattta
      601 gcgctggaat ttactccgcc gaggcggagg cgcatcggat ggtgctgcgc agcttttttt
      661 tatgccttcg gctacaatag gcgtcaggcg ggcgcagaca ttgtgcgttg cgccctgacg
      721 acctctctgc atcggggcgc ggagcgacgc gatctgagtg cgtcggcatt tgtgcgttat
      781 gcgttgagcc gcccgcacat tatcgccgta tcgcatcatc gccatgttgc cgcatgccct
      841 atgccgtgtg acttacagct atttgttttc caaaagccaa tgaggtgcat caatgatccg
      901 aacacctttt atgtcaggaa atgcgctgtg actgctatca gatgttataa tcaaaccttt
      961 gggaatttta aacgtttcca tcgcttctct taacccgtcc acttcacgct taatattact
     1021 gctgttggcc tgatgaacaa cctggattgc ttcggttatc ttcagaccat cccgcacaag
     1081 aaaatcacat tcgtttttac ctgaataata aaatacttcc ttacccctgc gcagcaattc
     1141 aaggtatatt atattttcaa gaatccggcc cgtattggcc gtaaaattaa acccaagccg
     1201 gtgcgcaaag gcctgatcgg ccacatatac ttttcgcgga ttcatcacct gctttttcaa
     1261 agagtaatca aatttcctca catagtgaaa caaatgagcc tgttcataat aaaaaagata
     1321 atcctttacc gaactcaggc tttttacacc ggaaatctgc tgcagcgttg agtacgaaaa
     1381 tacttttgat acgttggatg caaaatacaa cccgatctct tttatctcac taacctgcga
     1441 aagccggtat cgcgctacta tatcacgata taaaatatcg ctgaaatatg cgtttatcaa
     1501 ctccagatca tcttctttca acaccagcgg gaaacctccc atggagagaa aatgattgaa
     1561 atcaagattc agtaacgatc tggctttagt cgtcataccc gacaaatcat atgtatgacc
     1621 cataaaagtc aggtattcga caaatgaaaa cggaaacatt tctatttgct tgtttcgacc
     1681 ggtcagactt gccgctacat cggacaactg cagatttgca ttggagccgc tgatgaaaat
     1741 tttatttccg cgctcataca tccggtttac aaaacgctcc cagtgaagaa tgttctgaat
     1801 ttcgtccaga aacaacacgg gctctatgcc ataaagctcc aggtgcagat tatagaggtc
     1861 ttcgaatatt gaaacatctg caacaatgcg ctcatcatcg aaattgaaat aacagtattc
     1921 ggattctttc aatttcatct tttccttgat gaaatataaa agagtcgatt ttccacatcg
     1981 acgtatgcct gacactatca caatctgctt ggttgagatt gcctttcgca ggtcgatgtc
     2041 gcgctctgtc agagcggaac gccgattgaa gctttccttc tgatcgagca tgatttcttt
     2101 taaacggctc ttttccatag atattttcat tcgttatgaa cgaacaaata taatacattt
     2161 tattcgttta caacgaaata ttttgaatta tttttcgaag tttcgccaca aggacacgaa
     2221 gactctatga tttacgattt acgactcatt atttttgatt tttgatt
//
LOCUS       DAOPKE010000187         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_57546_length_2207_cov_11.220260, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000187 DAOPKE010000000
VERSION     DAOPKE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57546_length_2207_cov_11.220260"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKY63_12805"
     CDS             complement(<1..961)
                     /locus_tag="PKY63_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01539.1"
                     /translation="MSKEFHVIPFEQLLQMSLQQIDNGHFMGIPAGTFAGKPDPVIGI
                     RRFGQQLDTPIGVAAGPHSQLAQNIIAAWLCGARYIELKTVQTLDELNVSKPCIDMQD
                     EGYNCEWSQELKIHESFDQYLNAWIIIHLLNHKLHGQSQSGAGTIFNMSVGYNMEGIL
                     KDNVQWFFRKMQNCKAELDEKLEIVRKYYPDIDKINIPEQLSDNITLSTMHGCPPDEI
                     EKIGLYLIGNKKLHTVIKLNPTLLGPDMLRWILNGKLGFSTVVPDEAFAHDLKYQDAL
                     SIIRNLQVAAETSGVFFGLKLTNTLESMNIRGVFPENEKMNYMS"
     gene            1307..2116
                     /locus_tag="PKY63_12810"
     CDS             1307..2116
                     /locus_tag="PKY63_12810"
                     /inference="COORDINATES: protein motif:HMM:NF027222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HPF01540.1"
                     /translation="MPTYLFLFFIICLPSGIMAGNLLENNKDLQQIENKYNLNLIADS
                     FAGGDYAKNWAFWITPTDADSILFAEFMDVFAYEWNKYPLSWIKVNGLRGIAFVKNLT
                     VTGQERFAMPDPYDETLYYDIDYLRYGEDYVSEMIHHEFWHMIEEQHFGTMYYRSTTW
                     QSFNRSGFQYGNGGSDAYTDGEYTDGEHTKLGFVTNYAMYGEEEDRAETYCWFFTNRT
                     WTLLNKWISTDRLLKKKFNWMLNFMESKVPEMNFEYFEAINKAKVSLPLNK"
BASE COUNT          682 a          437 c          426 g          662 t
ORIGIN      
        1 cactcatgta attcattttc tcattttccg gaaaaacacc tctgatattc atgctctcca
       61 acgtattagt cagcttcaag ccaaagaaaa cgcccgaggt ttcagcagca acttgtaagt
      121 ttctgattat tgacaaagca tcctggtatt taaggtcatg tgcaaaagct tcgtcaggaa
      181 ctactgtgct gaagcccagt ttcccattca aaatccaacg cagcatatcg ggccccagca
      241 atgtgggatt gagttttatg actgtatgca gttttttatt cccaatcaga tataaaccga
      301 ttttttctat ttcgtcgggc gggcagccat gcatggtact taaggtaatg ttgtcggaaa
      361 gctgttccgg gatgttgatt ttatcaatat cggggtaata ttttctgaca atttcaagtt
      421 tttcgtcaag ctcagctttg caattttgca ttttccggaa aaaccattga acgttgtctt
      481 tcagaatgcc ttccatgttg tagccaacac tcatattgaa aatagtaccg gcaccagact
      541 gagattgccc atgcaattta tgattcaaca agtgaattat aatccaggca ttcagatact
      601 gatcgaacga ttcgtgaatt ttcaattcct gcgaccattc acaattatat ccttcgtcct
      661 gcatgtcgat acagggcttc gagacattca actcgtccaa tgtctgaaca gttttcaact
      721 caatgtatct tgcgccgcaa agccaggcag caataatatt ctgtgccagc tgcgaatgcg
      781 gacccgcggc caccccgatg ggagtatcga gttgttgtcc gaatcgcctg attccaataa
      841 ccggatctgg cttcccggca aaagtgccag caggaattcc cataaaatga ccgttatcaa
      901 tttgttgcaa cgacatttga agcaactgtt caaaaggaat aacatgaaat tctttcgaca
      961 tggttaaaat ttaattgccg aagatacgaa actcgtacgt gcaaatgcaa atctgataaa
     1021 attcaaactg attttaagag aattttaata tggaattctg ctgtgttcaa aaaaaatgaa
     1081 ttgaaaaaca actctgattt ttatacgaag atgtgatttt aaaaatcaca cttttgcata
     1141 aaataaatga ggttaatcat ttattaatca gatatttaaa taacagtaat tacaaataga
     1201 atcaaaatac catatcaggt tttattaatt cataacagcc attgtattgt tatctgtaat
     1261 tttgtgcaca aattttgtaa atctgatttt tttcaaattt gtcagtatgc caacctatct
     1321 gtttctgttt tttattattt gtttgccttc tggaataatg gcaggcaatc tgcttgaaaa
     1381 caacaaagac ctgcaacaaa tcgaaaataa atacaatctg aatctcattg ccgatagttt
     1441 tgcgggagga gactatgcaa aaaactgggc cttctggatt acgccaaccg atgcagattc
     1501 catcctgttt gctgaattta tggacgtatt tgcctatgaa tggaataaat atccgctttc
     1561 gtggattaaa gtaaacggac ttcgaggaat tgcctttgtg aaaaatctga cagtaaccgg
     1621 acaggaacgt tttgccatgc cggatcccta tgatgaaacc ttgtactatg acattgatta
     1681 tttaagatac ggagaagatt atgtgagtga aatgattcat cacgaattct ggcatatgat
     1741 tgaagaacag catttcggga caatgtatta ccggagcaca acctggcaat cctttaaccg
     1801 atcgggtttt caatatggca atggcggcag cgatgcttat accgatggtg aatataccga
     1861 tggtgagcat accaaacttg gctttgtgac caattatgcc atgtatggcg aagaagagga
     1921 ccgggccgaa acttattgct ggttttttac aaaccgaaca tggacactgc ttaataagtg
     1981 gatttcaaca gaccgccttc ttaaaaagaa attcaactgg atgctgaatt ttatggaaag
     2041 taaagtaccg gaaatgaatt ttgaatattt cgaagccatc aacaaagcca aagtttcttt
     2101 gccgctaaat aagtaggaat cgttatgtaa gtctccgcaa catttcacca aagccagact
     2161 ggaagccgca tttggttaca tcaatcacga cgcactcaga tcgcggc
//
LOCUS       DAOPKE010000188         2051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_63824_length_2051_cov_14.058617, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000188 DAOPKE010000000
VERSION     DAOPKE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63824_length_2051_cov_14.058617"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..480)
                     /gene="hypA"
                     /locus_tag="PKY63_12815"
     CDS             complement(142..480)
                     /gene="hypA"
                     /locus_tag="PKY63_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPF01541.1"
                     /translation="MHELSIAASIVDYATDFANEHQAKSISRIEIEVGQLSGVITDSL
                     EFAMEEAVKDSVLEKAEVVLTEVPGRSRCNRCQTEFDNPDWYTPCPNCMAMDAEIIGG
                     KELLITKISI"
     gene            578..1876
                     /locus_tag="PKY63_12820"
     CDS             578..1876
                     /locus_tag="PKY63_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPF01542.1"
                     /translation="MIYNIFIIATAVVFVLTIVFILNMLSVTRSRKSWVLISIAMLFL
                     IALQMIELYGQYFQIKDSFLQTIYYLLDFLVAILISVGVLQIGNLLKRLKNAEVERRV
                     SDRRFQILFDNSGDEIFLADFDGKFLEVNEEALKKLGYTREELMQKNFRDIKTEKYVP
                     IVDKNIAIILKNGHHVYETEHQAKDGTVIFLEMSDRVIDYMGRKAILSMARDITERKE
                     IERKIAAAIIETEERERKRFAADLHDGLAPLLSTIKLYTDLLKKGNFNKISPAETLQS
                     VDELIDKAIVSAREISNNIMPSILQDFGLPAATKDFVNYINNTNSVKIQLDTSQYKLT
                     GSRIEETVLFQAIKELVNNSLKHSQANNIEIFIESHDNQVNLLYKDDGVGFDVDEKLK
                     QPTGLGLNNIINKVKTINGLCLIKSAPGEGMNVLITVNVK"
BASE COUNT          617 a          408 c          446 g          580 t
ORIGIN      
        1 tttattaaca aaaatggcaa taataatttt gattttgaaa ttttagacag ttactcaatt
       61 ttggatgacc attgtttcag aattccggac ctgttcattt agaattgttt attcatgtat
      121 ttcaaaaaca acattttttg atcagatact gatttttgta atcagcaact cttttccacc
      181 aatgatttcg gcatccatgg ccatgcaatt ggggcagggg gtgtaccagt cgggattgtc
      241 gaattcggtc tggcaacgat tgcagcgtga gcggcccggg acctcggtca gtaccacttc
      301 ggctttctca agcacagagt cttttactgc ttcttccatg gcaaattcga gactatcggt
      361 aataactcct gacagttgac cgacttcaat ttcaattctg gaaatgcttt tcgcctgatg
      421 ttcgttggca aaatcggtgg catagtccac aatactggcg gcaatagata attcatgcat
      481 aatggataca aaattagagc aataaattga atggcaaaaa tatcttagcg atttcatcat
      541 tttccggata tcactatctt tgcgtcagaa gaatgctatg atttacaata ttttcatcat
      601 tgccacggcg gtcgtttttg ttctgaccat tgtatttatc ctgaacatgc tgtcggtcac
      661 ccgaagccgc aaatcgtggg tgctcatcag catagccatg ctgtttctga ttgcgctgca
      721 gatgattgaa ttgtatggtc agtattttca gatcaaagat tcatttttac agaccattta
      781 ttatctgctc gatttcctcg tagccattct catttcagtt ggggtgttac aaatagggaa
      841 tctgctgaaa agactcaaaa acgcagaggt agagcgaaga gtatcggacc gtcgctttca
      901 gattctattt gataattcgg gcgatgaaat ttttctggcc gatttcgatg gaaaattcct
      961 tgaggtgaac gaagaggcgt tgaagaagct tggctacacc cgcgaagagc ttatgcagaa
     1021 aaacttccgc gacattaaaa cagaaaagta tgttccgatt gttgataaaa acattgccat
     1081 tattctgaaa aacggacatc atgtgtacga aacagagcac caggcaaagg acggcaccgt
     1141 gatttttctg gaaatgagcg accgtgtgat tgattatatg gggcgtaaag ctattttgag
     1201 catggcacgc gacataaccg aacgcaagga aattgaacga aagattgcag ctgccattat
     1261 cgaaaccgaa gaacgcgaac gcaagcgttt tgctgctgat ctgcacgatg ggctggcgcc
     1321 gctgctttcg accattaagc tttataccga tctgcttaag aaaggaaatt tcaataaaat
     1381 cagtccggcc gaaacgctgc agtcggttga tgaactgatt gataaagcca tcgtttcggc
     1441 ccgagaaata tcgaacaaca tcatgcccag cattctgcag gacttcggac tgccggctgc
     1501 caccaaagat tttgtcaact acatcaacaa caccaactct gtaaaaattc aacttgatac
     1561 ttcgcagtac aaactcaccg gatccaggat tgaggaaacc gtattgtttc aggcaatcaa
     1621 ggaacttgtg aataattcgc tcaaacattc gcaggccaat aatattgaga ttttcataga
     1681 aagtcacgac aatcaggtga atcttttgta taaagacgac ggtgttggat ttgatgttga
     1741 tgaaaagctg aaacaaccca ctggtcttgg gctgaacaac atcatcaata aagtaaaaac
     1801 aataaacggg ttgtgcctta taaaaagcgc tcccggtgag ggtatgaatg ttctgattac
     1861 ggtaaatgta aaatagtcac catggatatt atccggttaa taatagctga tgatcatgaa
     1921 attttccgca aaggactgcg gattattctc aacgagctcg atgaagtgaa agtcgttgct
     1981 gaagcgcaaa atggcaacga agtgtttgaa attctgaaac atcagaaagc cgatctgatt
     2041 ctgatggaca t
//
LOCUS       DAOPKE010000189         1975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_67514_length_1975_cov_14.968750, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000189 DAOPKE010000000
VERSION     DAOPKE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67514_length_1975_cov_14.968750"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKY63_12825"
     CDS             <1..310
                     /locus_tag="PKY63_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01543.1"
                     /translation="QRQNVASGDDSGMGKLTINARCRHIWFSDQKICGQRKYSFRSAA
                     EKNHPDVCLRSGATSYIRLELTVYFPGNHIRRLAFLEFMPLKAVVLFLKFILSEVEGQ
                     "
     gene            434..670
                     /locus_tag="PKY63_12830"
     CDS             434..670
                     /locus_tag="PKY63_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01544.1"
                     /translation="MDSKIEIYKTTNGAEVAVQLEKDTVWLNRQQLTVLFNRDVKAIG
                     KHINNIFNEEELDPVSIVAKFAIVQEEGCFITEE"
     gene            709..1662
                     /locus_tag="PKY63_12835"
     CDS             709..1662
                     /locus_tag="PKY63_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HPF01545.1"
                     /translation="MSNEIEIFKSSDNKIELKVKFDSDTVWLSQEQLIVLFERDQSVI
                     SRHINNIFKEKELDRKSNMQKMHIAFSDKPVSFYSLDVIISVGYRVKSLRGTQFRQWA
                     TARLKDYLVQGYAINEKRLQQKQQEVEFLKTGLRIVSRAIEQAADEQEQEVFHQFARG
                     LALLDDYDHEALDKKGHTLKETVYPGFDDYMELIRLMYSDFKSSVFAKPKDESFHSSI
                     NQIKQSFSGTDLYPSIEEKAANLLYLITKNHSFVDGNKRIAAACFLLFLKRNHELFNS
                     KGEPIISNETLATLTLYIANSRSEEDDVVKRLIISVLNRNQ"
BASE COUNT          620 a          388 c          439 g          528 t
ORIGIN      
        1 ccaacgccag aatgtcgcct ccggcgacga ttccgggatg gggaaactca cgattaatgc
       61 acggtgtcgc catatatggt tttcggatca gaaaatatgc ggtcagcgga aatattcttt
      121 tcgaagcgca gcggagaaga accatccgga tgtctgtttg aggagcggag cgacgagtta
      181 tattcggttg gagctgaccg tttattttcc cggaaaccat atccggcgac tagcttttct
      241 ggagtttatg ccgctgaaag cggtagtact tttcttgaag tttatcctga gcgaagtcga
      301 aggacaatga aaaagaaaag tatgttcctg agataatttt ctatctttac tcaacgttgg
      361 attttcatgg gcggaagttt tcagccgaca tgtcccaaaa tggattgtgc ttcggaatta
      421 aaaatagtgg cgaatggatt ctaaaattga aatttataaa acaactaatg gagctgaagt
      481 tgctgtgcaa ttggaaaaag acacggtgtg gcttaatcgt caacagctga cagtcctgtt
      541 taatcgcgat gtaaaggcca ttggcaagca cataaataat atttttaacg aagaagagtt
      601 ggatccggtt tcaattgtcg caaaatttgc gatagttcaa gaagaaggtt gttttattac
      661 tgaagagtaa ttgaaaaagt gctgtgttta cgtctctaaa aattgaacat gagtaatgaa
      721 atagaaatat tcaaaagcag tgacaataaa atcgaattga aggttaagtt tgattcggac
      781 accgtttggc tttctcagga gcagcttata gtattgtttg aaagagatca atccgtgatt
      841 tcccgacaca tcaataatat tttcaaagaa aaggaattgg atagaaaaag caatatgcaa
      901 aaaatgcata ttgctttttc agataaaccg gtttcttttt atagtcttga tgttatcatt
      961 tcggttgggt atcgcgtaaa atcgctacga ggcacccagt tccgccagtg ggccactgcc
     1021 cggttaaagg attatctggt gcagggctat gccataaacg aaaaacgtct gcaacagaag
     1081 caacaggaag tggaatttct gaaaaccggt ttgcgcattg tgagccgcgc catagaacag
     1141 gccgccgatg agcaggaaca ggaggttttc caccagtttg ccagagggct ggccctgctc
     1201 gacgactacg atcacgaagc actggataaa aaaggacata ccctgaaaga aactgtttat
     1261 ccgggatttg atgattatat ggaactcatc cggctcatgt actccgattt taaatcttca
     1321 gtatttgcaa agcccaaaga tgaaagtttt catagttcaa tcaatcagat aaagcagagt
     1381 ttcagcggaa cggatttata cccaagtatc gaggaaaaag cagcaaacct gctatacctg
     1441 attacaaaaa accactcatt tgttgacggc aacaaacgca tcgccgccgc atgctttctg
     1501 ctttttctga aacgtaacca tgaattgttc aacagcaaag gtgaaccaat catttccaac
     1561 gaaacgcttg caacactcac gctgtatatt gccaacagcc ggtctgaaga agacgacgta
     1621 gtaaaacgct taattattag tgtcctgaac agaaatcaat aagctcaaca aatttaattg
     1681 ccatttgtga cttgaattca gcaatttcat acttaagata atttttcatg cccgaaacat
     1741 ctttccatac aacatattcc gacagacaat atctgctttc acaagcgaag aatggctctg
     1801 aaagcgggct gcggaagcgc gggtgtcacc gtttcggatt caaccacgcc gcagcgtggc
     1861 aagttcctca agagaatcag agtgagtgcg tagttgtcac ttgatcaaac gttgcctttt
     1921 aacttgatga ctcctatgaa taaaaccgcc taaaagcaag attaagagca gttta
//
LOCUS       DAOPKE010000190         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_70897_length_1909_cov_16.609493, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000190 DAOPKE010000000
VERSION     DAOPKE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70897_length_1909_cov_16.609493"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1164
                     /locus_tag="PKY63_12840"
     CDS             253..1164
                     /locus_tag="PKY63_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPF01546.1"
                     /translation="MKVSLKKYIWLSIAAAVATIALKTLAWQLTGSVGLLSDALESLV
                     NLVAAIIALVAITIAQRPADERHAFGHSKAEYFSSAIEGGLIILAAASIIVSAVPRLF
                     NPVEIENTGVGLLVSAGASLINLVVAVILIRAGKKHRSIVLEADGKHLMTDVWTSVGV
                     IGGIILVVLTGWLILDPIIAIAVAINIVISGAMLIKRSAQGLMDVSISDEDFIKVDKK
                     LGEYRKKGIEFHSLMTRQAGQRIFISVHVLVPGQWSVQKGHDLAEEIEKELIGLFKQP
                     VYVITHLEPIEDPCSLDDMELDRTFLK"
BASE COUNT          513 a          359 c          443 g          594 t
ORIGIN      
        1 ttgaatccga aacggtagtc ggccgaattt gaatttcttc ccggcattgg ggagccgaat
       61 ggatagtagt cggtgaaagc aatattaatg gcgaattgaa tgtgtggcat cgtttaattg
      121 atgtcagcta agataaatgt ttttgtaata tactttgttt ttttgtttaa aatccggtaa
      181 taacttttta ttttatttta ctgcagcaaa attctttctt tttatttttg gctattgaat
      241 aatctgtgct gaatgaaagt atcgctcaaa aaatacattt ggctttcaat cgcggctgca
      301 gttgcaacga ttgcgttgaa aacactggcc tggcaactga ccggatctgt ggggcttttg
      361 tccgacgctt tggaatcttt ggtaaatctt gttgcagcca ttattgctct ggttgctata
      421 accattgctc agcgtcctgc tgatgagcgt catgcttttg gtcacagcaa agcggaatat
      481 ttcagcagcg ccatcgaggg tggattgatt atacttgcgg cggccagcat tattgtttct
      541 gcggttccgc gactttttaa tcctgttgaa attgaaaata caggtgtggg tttgctggtt
      601 tcagcaggcg cttctttgat taatcttgtt gttgctgtta ttttaatccg tgccggaaaa
      661 aaacaccgct ccattgttct ggaagccgac ggcaaacatt tgatgactga cgtttggaca
      721 tcggttggtg taattggcgg aatcattttg gttgtgctca ccggatggct gatattggat
      781 ccgattattg ccattgccgt tgcaataaac atcgttattt ccggtgctat gctcattaag
      841 cgctctgcac aagggcttat ggatgtttca atcagtgatg aggattttat caaagtcgat
      901 aaaaaattag gcgagtatcg gaaaaaagga attgaatttc attcgttgat gacacggcag
      961 gcaggtcagc ggatttttat ttccgtacac gtgcttgtgc ccggccaatg gtctgttcag
     1021 aaaggacacg atctggcgga agaaatcgaa aaggaattga ttggtctgtt caaacagcct
     1081 gtgtatgtaa tcacgcatct ggagccgatt gaagatccat gctcgcttga tgacatggag
     1141 ctcgacagaa cgtttttgaa ataacgtttt acttttgcgc agatggataa aaattgtatt
     1201 gattaatgac ccctcctgaa aaaaaacgtt gccccagatg ttattgcgaa tttttttgca
     1261 atcacgaaaa cattgctctg tgtgattgct caaaaattaa aatgactgtg gccgagcgtg
     1321 aaaaaataag caaactatat tcggattgtc tttgtgtgaa atgtctcgaa gaactgctgc
     1381 tggagctccg taagcggatg ctttgaaact ggtaaacaca tccgatatgc gtgattggtt
     1441 atagacgcgg aatgacgcag aataaattta ttgtatgacc tgaaatagct tcatctctgt
     1501 gatctggcca ctgaggtact gaataccggg tccacggatt acactgaaaa cacttattcc
     1561 tcagcggcca tatcttgatg acacggacgt caacaattat tacgcagtaa taattgttga
     1621 ccacgaaaca tttgttcaac tatctgtgga aatggcttca gtcgcagact gcaagacttt
     1681 ttatccgcta aaatccaaag acctgctggg ggcattttaa tccgtgtaat ccgtgtcatc
     1741 tgtggaaaaa agcaagtgtc tctttacaac cactgaatca tgattcatcg cagattaaga
     1801 ctgattctac ttgtccgccc atcatgtcac ggtagtgact gatgctggcg ggttagtgtc
     1861 aatcgaaggg ccatacagag aaattaaaaa cgctttctgc gataattag
//
LOCUS       DAOPKE010000191         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_72470_length_1880_cov_17.641644, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000191 DAOPKE010000000
VERSION     DAOPKE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72470_length_1880_cov_17.641644"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1289)
                     /locus_tag="PKY63_12845"
     CDS             complement(153..1289)
                     /locus_tag="PKY63_12845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPF01547.1"
                     /translation="MENFIWNIPTQMHFGTDVCLKIRTILPIFGHKVMLLRGGESTVK
                     NGIRAKMLTELNSAGVEWIEFSGIKSNPEIEETRAAVEMGRGFRPDVILAVGGGSVID
                     TAKAVAAAIPVDNDPWDLYTGKLKPKTAIPVIAVLTLAATGTEMNPFSVMQNRAEGIK
                     TSFMSRLVAPKYSFLDPAFTVSVPRTYTAYGISDLVAHAMEAWFGEGDASLADRFVVA
                     IIQEAIEYGPRLLKEVDNVDMRARIMFAATCALNELTMFGRKSGDWGVHGLGHVFSLL
                     YGIPHGATLSAIFPAWMKHVATRDSARIAEFGKAVFGVSGVDQTIDRTVQMFRSFLSP
                     TNLGELKIEDYDEERVVDAMIRSEASGYHCFLGEEDFRAILGYM"
     gene            1342..>1880
                     /locus_tag="PKY63_12850"
     CDS             1342..>1880
                     /locus_tag="PKY63_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01548.1"
                     /translation="MIQGSGYVRRLNKKDADAVFAIRQEAYQSSAQFKVLNYSYLEWN
                     QVDDIAIVLGVFNEKDELMSSLRGEFIRTRSEAEEKLHYSIPKHLDLFPGILLGRGAT
                     KRTYRNYGFNSLLRYYYLGLAIDFPFKSTFASVFEKAPRIGLLRDMNYRFTIPSDAQD
                     NDIHVLTREYFVYLERKHFL"
BASE COUNT          502 a          511 c          367 g          500 t
ORIGIN      
        1 acacgctctt catcgtaatc atcaattttc aattcgctga gattggtcgg actcaatatt
       61 tcttttaact attctacaga tatatgactc cttcggagtc ctccgcaatt atgtgaaaaa
      121 ccgagaattt accggatatc attcattaca ctttacatat atccaagaat cgccctgaaa
      181 tcctcttcgc ccaaaaaaca atggtaaccg ctcgcctccg accggatcat tgcatcaaca
      241 acgcgctcct cgtcgtaatc ttcgattttc aattctccga ggttggtcgg actcaggaaa
      301 ctgcggaaca tctgcacggt ccgatcaatc gtttgatcaa cccccgaaac accaaatact
      361 gctttcccaa actctgctat gcgagctgaa tcacgagtgg caacatgctt catccatgca
      421 ggaaaaatgg ctgaaagcgt ggctccgtgc ggaattccgt acagcaacga aaaaacatgg
      481 ccaagtccgt gcacccccca gtcgccactt tttcgtccaa acatagtaag ttcgttcagc
      541 gcacaggtgg ccgcaaacat aatgcgggcg cgcatatcca cattgtccac ttccttcagc
      601 agccgaggtc cgtactcaat tgcttcctgt attatggcta ccacaaaacg atcggccaga
      661 ctggcatctc cttcaccaaa ccacgcctcc atggcatgag ccaccaaatc cgaaatacca
      721 taagccgtgt atgtgcgcgg gacacttacc gtaaaagccg gatcaagaaa actgtatttc
      781 ggcgccacca gccggctcat aaacgatgtc tttattcctt cagcacggtt ttgcatcaca
      841 ctgaacggat tcatttctgt accggttgca gccaatgtaa gaacagctat tacaggaatc
      901 gcggtctttg gtttcagctt tcccgtatat aaatcccatg ggtcattatc aacaggaatg
      961 gcagctgcca ctgcctttgc agtatcaata acactacctc cacctacagc caaaatcaca
     1021 tcgggccgga aaccgcggcc catctcaact gcagcgcgcg tttcttcgat ttccggattg
     1081 gatttaatcc ccgaaaactc aatccattca accccggcac tgttcagttc agtcaacatc
     1141 tttgcacgga tgccattttt caccgtcgat tcgcctccgc gcagcagcat cactttgtgt
     1201 ccgaatatgg gcagaattgt cctgattttc agacatacat cggtgccgaa atgcatttgt
     1261 gttggtatat tccagatgaa attttccatg ttttttttaa caaagttagg aatttgacga
     1321 aatcaatatt atttttagca catgattcaa ggcagcggtt acgtacgaag actgaataaa
     1381 aaagatgcgg atgcggtttt tgccatccgg caggaggcct atcaatcatc agcacaattc
     1441 aaagtactaa actattcata tcttgaatgg aatcaggtgg acgacattgc cattgtgctg
     1501 ggcgttttca acgaaaaaga tgaactgatg tcgtcgttgc gtggtgaatt tatccgcacc
     1561 cgctccgaag ccgaagaaaa gctgcattat tccatcccaa aacatctcga tctgtttccc
     1621 ggaatactgc tcggccgtgg tgccaccaaa cgcacctacc gcaactatgg cttcaattca
     1681 ttgctgcgct attattatct cggtctcgcc attgattttc catttaaatc aaccttcgca
     1741 tcggtattcg aaaaagcgcc ccgcatcggg cttctgcgtg atatgaatta tcgcttcact
     1801 attccgtcag atgcgcagga caacgatatt catgtgctca cccgcgaata tttcgtctat
     1861 ctcgaacgca aacatttcct
//
LOCUS       DAOPKE010000192         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_76111_length_1817_cov_14.043133, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000192 DAOPKE010000000
VERSION     DAOPKE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76111_length_1817_cov_14.043133"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKY63_12855"
     CDS             complement(<1..350)
                     /locus_tag="PKY63_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPF01549.1"
                     /translation="MAYFRLFRVVNLLIMALIMYFFRFFLFESALGFENIASPLSELQ
                     FGMLVGMFVLLAAGGYALNDYYDIGMDEINNPEKTVLRKKLPLSAGQNWFFILTAAGL
                     VIGFILAFMLKATSL"
     gene            complement(496..1017)
                     /locus_tag="PKY63_12860"
     CDS             complement(496..1017)
                     /locus_tag="PKY63_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPF01550.1"
                     /translation="MKNYREKLKNIRAFVFDYDGVLTNGNVYLTDSGMQLRQGNVRDG
                     YALQLARKKGFHIAVITGSDDRGIERRLNFLNIEQVYLGVSDKKKVFENYLETYQLLP
                     SEILFMGDDLPDYPIMKMAGIPCCPADASEEIKQVSEYISPLQGGRGCVRDVIEQVLK
                     IKGMWMDDDAFHW"
     gene            complement(1001..>1817)
                     /locus_tag="PKY63_12865"
     CDS             complement(1001..>1817)
                     /locus_tag="PKY63_12865"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPF01551.1"
                     /translation="MTKIAYFCCTMSALKNHIVVIGAGNVGTHLALLFKENGAEVTVA
                     CRNSASAAHLSEKGISVVSISEMTIDAALYLLCLKDSVVPEVAAVLPDLRGIVAHTSG
                     SLGQEPLSNCSNKGVFYPFQSFRKEIPLTVKDFPILIEASDEATESVLLHFANMISSS
                     AMVAPGNVRAGIHAGGVFVSNFTNLMYNAGWQVAQEKGFDANKMLRPLIEETALRLKY
                     KHPSELQTGPAIRNDRETVSKHLEILKENPELFTLYKELTNVLLKKYGHEELS"
BASE COUNT          548 a          440 c          319 g          510 t
ORIGIN      
        1 agcgaagttg ctttcagcat aaaagccaaa ataaaaccga taaccagccc tgcggctgta
       61 agaatgaaaa accagttctg tccggccgac aatggaagct tttttcgcaa taccgtcttt
      121 tccggattat tgatttcatc cataccaata tcgtaataat cattcagtgc ataaccacca
      181 gctgccagca aaacaaacat gcccaccagc attccgaact gaagctcgct taagggagat
      241 gctatatttt caaaacccag cgctgattcg aaaagaaaaa aacggaagaa atacatgatc
      301 agcgccatga taagcagatt cacaactctg aaaagacgaa aataggccat tgcaatttat
      361 ttttactaat aactttttta tcactgtttt attttacttc tctccccctg tcatcctgag
      421 tgtccttcat ctgctgtcat tctgagtgaa ttttttcgca gtgaaacgaa gaaaaaatta
      481 gtatcgaagg atgacctacc aatgaaacgc atcatcatcc atccacattc ccttgatttt
      541 aagcacctgc tcaatcacat ctctaacaca ccctcttcca ccctgcaagg gagaaatata
      601 ttcgctcacc tgcttgattt cttcactggc atcagccgga caacaaggga tcccagccat
      661 tttcataatt ggataatccg gaagatcatc acccataaac aaaatttcag atggcaaaag
      721 ctgataagtt tccaaataat tttcaaaaac tttttttttg tccgagacac ccagatacac
      781 ctgttctata ttcagaaaat tcaaacgtcg ttcaatacct cgatcatctg aaccagtaat
      841 aacagcaata tgaaaacctt tttttcttgc cagctgcaat gcatatccgt cgcgcacatt
      901 gccctgtcgg agttgcattc cggaatcagt cagatacaca tttccgttgg ttaatacccc
      961 atcgtaatca aacacaaatg ccctgatatt ttttaatttt tcacgataat tcttcatgtc
     1021 cgtatttttt cagcaacaca tttgtgagtt ctttgtaaag tgtgaacaat tccggatttt
     1081 ccttcaaaat ttccagatgc ttcgagactg tttcacggtc attgcgaatg gctggccccg
     1141 tctgcaattc tgatggatgc ttatatttca gccgcaatgc tgtttcttct atcaatgggc
     1201 gaagcatttt attggcatca aatccttttt cttgtgctac ctgccaaccg gcattataca
     1261 tcagattcgt aaaattcgat acaaaaacac cacccgcatg aatgcccgct ctgacattgc
     1321 caggggccac catcgccgat gaggaaatca tgttggcaaa atgcagcaaa actgactctg
     1381 tggcctcatc cgatgcttcg ataagaatgg gaaaatcctt cacggtaagt ggaatctctt
     1441 tgcgaaagga ttgaaacgga taaaaaacgc ctttgtttga acagtttgaa agcggctcct
     1501 gacctaaact tccggatgta tgtgccacaa tgcctcttaa atcaggcagc actgctgcga
     1561 cctcaggaac aacggaatct ttcaggcaga gcagatataa agcggcatcg atcgtcattt
     1621 cggatattga aacaacggaa ataccttttt cggaaagatg cgcagcagat gcactgttgc
     1681 gacaggctac cgtcacttcc gctccattct ccttaaaaag caatgccaga tgtgttccca
     1741 cgtttccggc acctataacg acaatatgat ttttcaaagc actcatagtg caacaaaagt
     1801 aagcaatttt ggtcatt
//
LOCUS       DAOPKE010000193         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_78461_length_1780_cov_16.271304, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000193 DAOPKE010000000
VERSION     DAOPKE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78461_length_1780_cov_16.271304"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKY63_12870"
     CDS             complement(<1..501)
                     /locus_tag="PKY63_12870"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPF01552.1"
                     /translation="MCYLKNAEYKSNYIIRFTFDKKRVREIDFEPFLLFMKDHPMIAP
                     YLDLKKFRNFSWDASTIEWNDFEMCFSAESLFQDDWSAEGVVKSRIEMFRNAFSKAII
                     DLEQYVRYFPTKHDKIGFTDWELDDLLVVFPKITKKKFYKAMGVYSAMEIDGEPVLYR
                     HDVLLAI"
     gene            complement(489..929)
                     /locus_tag="PKY63_12875"
     CDS             complement(489..929)
                     /locus_tag="PKY63_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01553.1"
                     /translation="MENLLRIKREFLKPNDILVFNQGNNRVVTRWFDHTSFLIEENQH
                     LLICLDHSLGDDEGIIRMDLIYVDTIVKPTLHGYPEYSLSYTIRLPRGGFQKNSEVEI
                     PFENNYGLRQLQRFVFSHHAAKRGMKIYTSVDLATKKQIAPCVI"
     gene            complement(939..1355)
                     /locus_tag="PKY63_12880"
     CDS             complement(939..1355)
                     /locus_tag="PKY63_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01554.1"
                     /translation="MEQLEQLERLTRTGRLDKTIRKMLLKTFPEEAKRTTLFCEYHAN
                     PTYVNGGWCRINKTTFLVNAATGARIQLDTAYNIPFAPDYKMMGSVNAVLNFVLFFPP
                     VPASWTTFHLIEECPDNPFVVKNIERNNKGVYNVTL"
BASE COUNT          474 a          390 c          379 g          537 t
ORIGIN      
        1 gatggccagc aatacatcat ggcggtacaa caccggctct ccgtctattt ccattgcaga
       61 gtaaactccc attgccttgt agaatttctt ttttgttatt ttcggaaaaa caaccagtaa
      121 atcatcgagc tcccagtccg taaatccaat cttgtcatgt tttgttggaa agtacctgac
      181 atattgctcc agatcaatga ttgctttact gaaagcattc cggaacattt caatccttga
      241 tttcacaacg ccttcggctg accagtcatc ctggaaaaga ctttcggctg aaaagcacat
      301 ttcgaaatcg ttccactcaa ttgttgaagc atcccaggaa aagtttctga attttttcaa
      361 atccagatat ggcgcaatca tcggatgatc tttcatgaac aatagaaacg gctcaaaatc
      421 aatttccctt acccgctttt tatcaaaggt gaagcgaata atgtaattgc ttttgtattc
      481 cgcgttcttt aaataacaca tggcgctatc tgttttttag tagccaaatc aacacttgta
      541 tagattttca tccctcgttt tgccgcatga tgcgaaaaca cgaatcgctg aagctgtctc
      601 aatccgtagt tgttttcgaa cggaatttcc acttcgctgt ttttctgaaa tccgcccctg
      661 gggaggcgga tagtgtaact caagctgtac tccggataac catggagggt cggttttaca
      721 atcgtatcaa cataaatcag gtccattctg ataattccct cgtcgtctcc cagtgaatga
      781 tccagacaaa tcagcagatg ctggttctcc tctatcagaa aggaggtgtg gtcaaaccat
      841 cgagtgacaa ctctgttgtt tccctggttg aaaacaagga tgtcattggg tttcagaaac
      901 tcgcgtttaa ttctaagcag attttccatg gggttgaatt ataaagtgac gttgtaaact
      961 cctttgttgt ttcgttcaat atttttcacg acaaatggat tgtcgggaca ttcttcaatt
     1021 aagtgaaatg tggtccagga tgccggaacg ggcgggaaaa agagaacgaa attcagtacc
     1081 gcgtttaccg atcccatcat tttataatcc ggcgcaaagg gaatgttata tgccgtgtcc
     1141 agttgaattc ttgcacccgt tgcggcatta accagaaatg ttgttttatt gatccggcac
     1201 cagccgccat ttacgtaggt cggatttgca tgatattcgc aaaacaaggt ggtgcgtttt
     1261 gcctcttcgg gaaaggtttt cagaagcatc ttccggattg ttttgtcaag acgtccggtg
     1321 cgggtaagtc tttctaattg ttcaagctgt tccatgactg agtttttcac aaagaaaaaa
     1381 cacctgaacg caatcagtct gcgttttgct tttcgcgagg aagggattca acagagtcta
     1441 caaactttgc cctctttttg gtccacggat tacactgatt gcacggaaat acttttgctt
     1501 cagcggcccc atcaggatta cactgttgtc aataattatt actgcgcaat aattatcgac
     1561 ggcaaaatgc ttttgtgtat ttccgtggaa atggcttcag tcgaagactg aatgacattt
     1621 catccgctga aatccaaaga cctgctgagg ggctgttaat ctgtggaatc tgtgtaatca
     1681 gtggacaagc gaagcttctg gttcagtgct tcgaaggttt ccgatgagat cccgggtcac
     1741 atgaactctg aacagcgaac ctgtctgcaa caggcaggca
//
LOCUS       DAOPKE010000194         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_84206_length_1697_cov_11.092570, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000194 DAOPKE010000000
VERSION     DAOPKE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84206_length_1697_cov_11.092570"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKY63_12885"
     CDS             <1..573
                     /locus_tag="PKY63_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01555.1"
                     /translation="IAVSMLMTSILKMRWMIFSGNVVFIVYGVMIGAYPIILLNGFNM
                     VVNAYFIWQAYKLQGNYAVLPSNMTSPMMERFLVYYADDIRKYFPAFQRFEKEDIVFL
                     IVKGTAVISVAGFRKNNDGSFNVVVDYVAKTHRNMKPGKLLFERENIFSLLNTDTIYA
                     QSYHLRHTVYLHRMNFKPTETKDVFVLQKH"
     gene            complement(616..>1697)
                     /locus_tag="PKY63_12890"
     CDS             complement(616..>1697)
                     /locus_tag="PKY63_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPF01556.1"
                     /translation="ALTPEDKAFRMNRPQTPQPPYPYICKDVTFVNKKAKINLAGTLT
                     IPDTTKVWPVVVLVSGSGPQDRNEELLKHQPFLIIADYFTRNGIAVLRYDDRGVGKSE
                     GKFNEATTADLSSDAGSAVSFLKTLPYIDKKAIGMVGHSEGGVIVLMQASQNKSLRFV
                     ISMAGVSIPCLQLLVMQNDKIMEGYGAEEELRDFFYTFNTALYNLVLTEKNNDSLRSK
                     ALKLFNESSTSLSEEQKNEYNLNETYVSAMLMQLTTPWFRYFLSIIPSNYIKKIKTNV
                     LAINGSKDVQVPAEANIEAFNTYFVQQRGIFKETHVFPGLNHVFQPCTTGMTTEYAMI
                     ETTIDPPVLEYMTTWIKKVLATGKK"
BASE COUNT          426 a          329 c          386 g          556 t
ORIGIN      
        1 atcgctgttt cgatgctgat gacatcgatt ttgaaaatgc ggtggatgat tttttcgggc
       61 aatgtggttt ttattgttta tggtgttatg attggcgcct atccaattat attgctgaat
      121 ggttttaata tggtggtgaa cgcttatttc atctggcagg cctacaagtt gcaggggaat
      181 tacgcagtat tgccatcaaa catgacttcg ccgatgatgg agcgttttct ggtgtattat
      241 gccgatgata tccgtaagta ttttccagct tttcagcgtt ttgaaaaaga ggatattgtt
      301 tttttgattg ttaaaggcac tgctgtaatc agtgtggccg gattcagaaa aaacaatgat
      361 ggaagtttta atgttgttgt ggattatgtt gcaaaaacgc accgtaacat gaaacctggc
      421 aaactgcttt ttgagcgcga aaatattttt tcgttgctga ataccgatac catctatgcg
      481 cagtcgtatc atttgcgaca taccgtttat ctgcacagga tgaatttcaa accaaccgaa
      541 accaaggatg tgtttgtgct gcaaaagcat taaacaatcc ctcgaagagt ttaaaaccct
      601 ttgcgggatt ttgtcctatt tcttcccggt tgcaagcact ttttttatcc atgtcgtcat
      661 atattccaga accggtggat cgatagttgt ttcaatcatt gcatattcag ttgtcattcc
      721 tgtggtgcat ggctggaata catggttcag ccccggaaat acgtgggttt ccttgaaaat
      781 gccacgttgt tgtacaaaat aggtattgaa tgcctctatg ttggcttcag cagggacctg
      841 aacatctttc gatccattga tggccagtac attggttttg atttttttga tgtaattcga
      901 tggaatgatg gaaagaaaat atctgaacca gggtgttgta agctgcatca gcattgcact
      961 tacgtaagtt tcattcagat tatactcgtt tttttgttct tctgaaagcg aagttgatga
     1021 ttcgttaaat aattttagtg ctttgcttcg cagactgtca ttattttttt ctgtcagcac
     1081 gagattgtag agggcggtat taaaagtgta gaaaaaatcg cgtagttctt cttctgcgcc
     1141 gtatccttcc atgattttgt cgttttgcat tacaagcaat tgcagacagg gaatggatac
     1201 tcctgccatc gatatcacaa agcgcagcga cttgttttgc gaggcctgca tcagaacaat
     1261 gacgccacct tcgctgtgac cgaccatgcc aatggctttt ttatcgatgt aaggcaatgt
     1321 tttcagaaag gatactgcag aacccgcatc ggagctcaaa tcagcagtcg ttgcttcatt
     1381 gaatttccct tcgctttttc caacaccacg gtcatcgtaa cgtaaaacag caatgccatt
     1441 tcttgtgaaa taatcagcaa taatgagaaa tggctgatgt ttcagcaatt cttcgttcct
     1501 gtcctgcggg ccgcttcctg aaaccagcac cacaacaggc catactttcg ttgtgtctgg
     1561 aatggtcaat gtgccggcca gattgatttt cgcttttttg ttgacgaaag ttacgtcctt
     1621 acagatgtaa ggatagggtg gttgcggcgt ttgcggacgg ttcatgcgga aagccttgtc
     1681 ttcgggtgtc agagcca
//
LOCUS       DAOPKE010000195         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3259
            NODE_100620_length_1502_cov_14.288874, whole genome shotgun
            sequence.
ACCESSION   DAOPKE010000195 DAOPKE010000000
VERSION     DAOPKE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864994
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:07:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,547
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100620_length_1502_cov_14.288874"
                     /isolate="AMR_MDS_3259"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..764
                     /locus_tag="PKY63_12895"
     CDS             51..764
                     /locus_tag="PKY63_12895"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPF01557.1"
                     /translation="MKTKFFPLLLIAMIFVSQSCFHPYRIEGNCNVVTVNRSIGEFTG
                     VDSRGSLDVRLINDSVCFVEIEGESNLIPYVDTDISNGILQIEVQGWRNLDPNYPLII
                     YVHAPSINHVELSGSGSIVSDSLSASHMDIRLSGSGDIHLKLQASSVDAKISGSGDIN
                     LEGAAALADFRISGSGDIYAYSFPVNECMADISGSGSMYVNVSSLLDVVISGSGNVHY
                     YGNPAVNVHVFGSGQVIHH"
     gene            774..1271
                     /locus_tag="PKY63_12900"
     CDS             774..1271
                     /locus_tag="PKY63_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01558.1"
                     /translation="MKRILLLFFSIGLSLNVFSQSFDMEVGLRSGVSSGFSLRVYTGY
                     SDWAEGLLLGRDGGLQLTVLKGKSIPYLIGHHIPLTLQTAFGGHIGNVRSDYRSGEYY
                     YNRRCLPVAGVDFFLAATYQFPRFPLSLSLDYKPFAELNPDRFLHINLWDFGFTVRYQ
                     FTSKK"
     gene            1276..>1502
                     /locus_tag="PKY63_12905"
     CDS             1276..>1502
                     /locus_tag="PKY63_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF01559.1"
                     /translation="MKKVLISFSAILLLTLVSTAQNDSIRSSEIKTLFGNRDHSNGWY
                     VGLGGGYSQIDGHNAFTTSFRSAWIINHSFA"
BASE COUNT          357 a          295 c          338 g          512 t
ORIGIN      
        1 ttttgcaacc cttcattttt tcaaaagaca ttaggtaaat gtctgtcaaa atgaaaacaa
       61 aattttttcc tttactgttg attgcgatga tttttgtgtc gcaatcctgc tttcatccat
      121 acaggattga aggcaactgc aatgtggtta ctgtgaatcg ttctattggc gaatttacag
      181 gcgtcgatag ccgtggttca ctcgacgtga ggttgataaa tgattccgtt tgctttgtcg
      241 agattgaagg cgaatccaat ctgattcctt atgttgatac cgatatcagc aatggaattc
      301 tgcaaattga agttcagggc tggcgaaatc ttgacccaaa ttatcctttg attatttatg
      361 tgcatgctcc ttctatcaat catgtggagc tttcgggtag cggaagcatt gtctcggact
      421 cgctttcagc ttcgcatatg gatattcgcc tgtcgggttc cggcgatatt catctgaaac
      481 ttcaagccag ttcggtcgat gcaaaaattt cgggctccgg cgatattaat cttgagggtg
      541 ccgctgcttt agccgatttc agaatttcgg gttccggcga tatctatgca tacagttttc
      601 ctgtgaatga atgcatggcc gatatatccg gctcaggtag catgtatgtc aatgtttctt
      661 cattgctcga tgttgttatc agtggcagtg ggaatgtaca ttattatggc aatccggcgg
      721 tcaatgtaca tgtgtttggc agtggacagg ttattcacca ttgatgtttg attatgaaaa
      781 gaatattatt gttgtttttt agcattggcc tgtcactgaa tgttttttca caaagctttg
      841 atatggaagt gggcttgcgt tcaggggtca gttcgggttt ttcgttacgc gtttatactg
      901 gttacagcga ttgggctgaa ggacttttgc tgggccgtga cggtggatta caactgacag
      961 tcttgaaagg gaaaagtatt ccttatctta ttggtcatca cattccgctt actttgcaga
     1021 ctgcatttgg cggtcatatc ggaaatgtac gctctgatta tcgctcaggt gaatattatt
     1081 acaatcgccg gtgcttaccg gttgctggtg tcgatttttt tcttgccgca acctatcagt
     1141 tccctcgttt tccacttagt ctttcgctcg attataagcc atttgcagag ttaaatcccg
     1201 atcgtttttt gcatataaat ctctgggatt ttggttttac agttcgttat cagttcactt
     1261 caaaaaaata aaaaaatgaa aaaagttctc atatcatttt cagctattct gttgttgact
     1321 ttggtttcaa cagcgcaaaa cgacagcatc cggtcttctg aaatcaaaac cctttttggc
     1381 aaccgcgacc acagcaatgg ctggtatgtg ggcttgggtg gcggttattc acaaattgac
     1441 gggcacaatg cttttacaac ctcattcagg tcggcctgga ttattaatca cagctttgcc
     1501 at
//
